LOCUS       DAZPEB010000001        81870 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZPEB010000001 DAZPEB010000000
VERSION     DAZPEB010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 81870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 81870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..81870
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.001"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            40..582
                     /locus_tag="ACHURJ_000001"
     CDS             40..582
                     /locus_tag="ACHURJ_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1279109.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTSVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
     gene            662..1336
                     /locus_tag="ACHURJ_000002"
     CDS             662..1336
                     /locus_tag="ACHURJ_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFR1279110.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWHLTIIDDHGN
                     YISDKI"
     gene            1352..3832
                     /locus_tag="ACHURJ_000003"
     CDS             1352..3832
                     /locus_tag="ACHURJ_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFR1279111.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYL
                     SQYYSDYKASGNNKSTYVRFNSGLNLLGWQLHSDASFSKTNNNPGGWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFRGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSEFVQVGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYTLAASQAYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVIYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLMFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            3848..4882
                     /locus_tag="ACHURJ_000004"
     CDS             3848..4882
                     /locus_tag="ACHURJ_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HFR1279112.1"
                     /translation="MEIRIMLFILMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     MATYEHNFNDSSCVSTNTITPMSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTNGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLEGIPVSQLPATGN
                     KATINSKKGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLTNSNTILKGAEDNGVGFIL
                     ESNGSPVTLLNITDNSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALE
                     ATALINVKYD"
     gene            complement(4964..5302)
                     /gene="rcnB"
                     /locus_tag="ACHURJ_000005"
     CDS             complement(4964..5302)
                     /gene="rcnB"
                     /locus_tag="ACHURJ_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFR1279113.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(5521..6345)
                     /gene="rcnA"
                     /locus_tag="ACHURJ_000006"
     CDS             complement(5521..6345)
                     /gene="rcnA"
                     /locus_tag="ACHURJ_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFR1279114.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIISTA
                     FWMFWRTWRGERNWLENMHGHDYEHHHHDHEHHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            6466..6738
                     /gene="rcnR"
                     /locus_tag="ACHURJ_000007"
     CDS             6466..6738
                     /gene="rcnR"
                     /locus_tag="ACHURJ_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFR1279115.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      6830..6926
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6961..7749
                     /gene="thiM"
                     /locus_tag="ACHURJ_000008"
     CDS             6961..7749
                     /gene="thiM"
                     /locus_tag="ACHURJ_000008"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFR1279116.1"
                     /translation="MQVDLLGSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEMDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            7746..8546
                     /gene="thiD"
                     /locus_tag="ACHURJ_000009"
     CDS             7746..8546
                     /gene="thiD"
                     /locus_tag="ACHURJ_000009"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFR1279117.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            8611..9429
                     /locus_tag="ACHURJ_000010"
     CDS             8611..9429
                     /locus_tag="ACHURJ_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFR1279118.1"
                     /translation="MQLRITSRKKLTSLLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQVRLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQAFVDGIKETP"
     gene            9481..10227
                     /locus_tag="ACHURJ_000011"
     CDS             9481..10227
                     /locus_tag="ACHURJ_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR1279119.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(10201..11166)
                     /locus_tag="ACHURJ_000012"
     CDS             complement(10201..11166)
                     /locus_tag="ACHURJ_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFR1279120.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(11163..12167)
                     /locus_tag="ACHURJ_000013"
     CDS             complement(11163..12167)
                     /locus_tag="ACHURJ_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFR1279121.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRTTTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            complement(12164..13441)
                     /locus_tag="ACHURJ_000014"
     CDS             complement(12164..13441)
                     /locus_tag="ACHURJ_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFR1279122.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMSTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            13698..14750
                     /gene="fbaB"
                     /locus_tag="ACHURJ_000015"
     CDS             13698..14750
                     /gene="fbaB"
                     /locus_tag="ACHURJ_000015"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFR1279123.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVVSTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            15058..15912
                     /gene="gatY"
                     /locus_tag="ACHURJ_000016"
     CDS             15058..15912
                     /gene="gatY"
                     /locus_tag="ACHURJ_000016"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFR1279124.1"
                     /translation="MHVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATEPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            15941..17143
                     /gene="gatZ"
                     /locus_tag="ACHURJ_000017"
     CDS             15941..17143
                     /gene="gatZ"
                     /locus_tag="ACHURJ_000017"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFR1279125.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKEYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIVRGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQPLAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPH"
     gene            17213..17665
                     /gene="gatA"
                     /locus_tag="ACHURJ_000018"
     CDS             17213..17665
                     /gene="gatA"
                     /locus_tag="ACHURJ_000018"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFR1279126.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVEVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            17696..17980
                     /gene="gatB"
                     /locus_tag="ACHURJ_000019"
     CDS             17696..17980
                     /gene="gatB"
                     /locus_tag="ACHURJ_000019"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFR1279127.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            17984..19339
                     /locus_tag="ACHURJ_000020"
     CDS             17984..19339
                     /locus_tag="ACHURJ_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFR1279128.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSVSLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFITWRRARGFIKQEKAVLA
                     E"
     gene            19387..20427
                     /gene="gatD"
                     /locus_tag="ACHURJ_000021"
     CDS             19387..20427
                     /gene="gatD"
                     /locus_tag="ACHURJ_000021"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFR1279129.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGSMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            20533..21306
                     /locus_tag="ACHURJ_000022"
     CDS             20533..21306
                     /locus_tag="ACHURJ_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR1279130.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSVSGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(21388..22286)
                     /gene="yegS"
                     /locus_tag="ACHURJ_000023"
                     /pseudo
     CDS             complement(21388..22286)
                     /gene="yegS"
                     /locus_tag="ACHURJ_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
     gene            22700..23017
                     /gene="yegR"
                     /locus_tag="ACHURJ_000024"
     CDS             22700..23017
                     /gene="yegR"
                     /locus_tag="ACHURJ_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFR1279131.1"
                     /translation="MKKIAAISLISIFIMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARSRISGAENIVKNDATHFCVTQGKKMQIVELKTEGVGLHGVARLTFK
                     CGE"
     gene            complement(23347..24708)
                     /gene="yegQ"
                     /locus_tag="ACHURJ_000025"
     CDS             complement(23347..24708)
                     /gene="yegQ"
                     /locus_tag="ACHURJ_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFR1279132.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREYFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGVAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(24850..25008)
                     /locus_tag="ACHURJ_000026"
     CDS             complement(24850..25008)
                     /locus_tag="ACHURJ_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFR1279133.1"
                     /translation="MTNVPADERNQNETIREALRLLREKLAEGLRSGEAQPWEKDLFL
                     HKVKAGNK"
     gene            complement(25029..26429)
                     /locus_tag="ACHURJ_000027"
     CDS             complement(25029..26429)
                     /locus_tag="ACHURJ_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000911981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HFR1279134.1"
                     /translation="MLKGLRTLFSRRVLFNVEMNNSVDCELLLIQQQLQALIAEPGNY
                     LAHDCDLNDTLVLCQLTHHHNGYIREKAVQYLGHKEDVAAIEELLLAANDWVKEVRLA
                     ATIALRHLMKDKNAAAFAVNLPMIRNLLQCQRYDHRAFVDEILRYLLTENNRPQLTYW
                     LTCPDKKLSRAVLQTLIEYGYFNDESSLLSILDKPDEGLRMLATTHWLRQQLPLSEAV
                     LTRLLKDRWPRIRQATLFSLTDRAIEMPPALHSTLLLDNNMLIRLRAKNMLHEVMDVP
                     QFWRHVVTSAEYTPSQRRAALYGLDSIHDPNILKLAEWGLSQNVFPLRLAAMHILAKA
                     NPRCGVKETILTTLANPDAAGLRFMVNICVWCRVPLTFEEIRQLQENAPSVKHACAYC
                     RLYHNLNKWDGLILLLQSQHKLTEEFAGKQLAIWQRNFNLSGIQPNALQRQQLQALFT
                     RNPELHNRLWGYIPFK"
     gene            complement(26552..26884)
                     /locus_tag="ACHURJ_000028"
     CDS             complement(26552..26884)
                     /locus_tag="ACHURJ_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFR1279135.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            complement(27075..27797)
                     /gene="baeR"
                     /locus_tag="ACHURJ_000029"
     CDS             complement(27075..27797)
                     /gene="baeR"
                     /locus_tag="ACHURJ_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFR1279136.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            complement(27794..29197)
                     /gene="baeS"
                     /locus_tag="ACHURJ_000030"
     CDS             complement(27794..29197)
                     /gene="baeS"
                     /locus_tag="ACHURJ_000030"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFR1279137.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDEQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            complement(29194..30610)
                     /locus_tag="ACHURJ_000031"
                     /pseudo
     CDS             complement(29194..30610)
                     /locus_tag="ACHURJ_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(30611..33688)
                     /gene="mdtC"
                     /locus_tag="ACHURJ_000032"
     CDS             complement(30611..33688)
                     /gene="mdtC"
                     /locus_tag="ACHURJ_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFR1279138.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGKLYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVEPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            complement(33689..36811)
                     /gene="mdtB"
                     /locus_tag="ACHURJ_000033"
     CDS             complement(33689..36811)
                     /gene="mdtB"
                     /locus_tag="ACHURJ_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFR1279139.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLIFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYSAFGQRLISTIYTQANQYRVVLEHNTEITPGLAALDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            complement(36811..38058)
                     /gene="mdtA"
                     /locus_tag="ACHURJ_000034"
     CDS             complement(36811..38058)
                     /gene="mdtA"
                     /locus_tag="ACHURJ_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFR1279140.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSRSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTIIAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGMIKVKARFNNQNDVLFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            38337..38393
                     /locus_tag="ACHURJ_000035"
     CDS             38337..38393
                     /locus_tag="ACHURJ_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR1279141.1"
                     /translation="MMKLLIIVVLLVISWPAY"
     gene            38665..38721
                     /locus_tag="ACHURJ_000036"
     CDS             38665..38721
                     /locus_tag="ACHURJ_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR1279142.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            38994..39053
                     /gene="ibsA"
                     /locus_tag="ACHURJ_000037"
     CDS             38994..39053
                     /gene="ibsA"
                     /locus_tag="ACHURJ_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HFR1279143.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            39274..39933
                     /locus_tag="ACHURJ_000038"
     CDS             39274..39933
                     /locus_tag="ACHURJ_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFR1279144.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            39930..40691
                     /gene="pphC"
                     /locus_tag="ACHURJ_000039"
     CDS             39930..40691
                     /gene="pphC"
                     /locus_tag="ACHURJ_000039"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HFR1279145.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVVWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAFMVQKVQDGELGLNDVLATNMVLTIRQRLFAEAEA
                     KALAVRDFACTFLGLISSPDGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTGRAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASVTQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            40688..42610
                     /gene="yegI"
                     /locus_tag="ACHURJ_000040"
     CDS             40688..42610
                     /gene="yegI"
                     /locus_tag="ACHURJ_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HFR1279146.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDIEEFVDSVAKIYHTP
                     PPASKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQSYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINVNGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDSLRQQLKKCPVSAMHVYPAHLTDCPWCTLDNQGVIY
                     FVDLGEEVITTSGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILIEIALSALSLLLCGLQAEPRYIILVPVLSAIWIIGSLTSKAYKAEIQQRREAFNRA
                     KMDYEHLVSQIQQLGGLEGFIAKRAMLEKMKDEILGLPEEEKRALAALYDTARERQKQ
                     KFLEGFFASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWKASC
                     DRRFVFRPNEAVTPADRQAVMAAKRHRLESALTVGATELQRFRLYAPARTMPLMEPLR
                     QAAEKLAQAQADLSRC"
     gene            complement(42622..43974)
                     /gene="yegD"
                     /locus_tag="ACHURJ_000041"
     CDS             complement(42622..43974)
                     /gene="yegD"
                     /locus_tag="ACHURJ_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR1279147.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEESKIALSNAAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGISIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            44108..44965
                     /gene="alkA"
                     /locus_tag="ACHURJ_000042"
     CDS             44108..44965
                     /gene="alkA"
                     /locus_tag="ACHURJ_000042"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFR1279148.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADDYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDGTDEL"
     gene            complement(45004..48301)
                     /locus_tag="ACHURJ_000043"
                     /pseudo
     CDS             complement(45004..48301)
                     /locus_tag="ACHURJ_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            48619..49260
                     /gene="udk"
                     /locus_tag="ACHURJ_000044"
     CDS             48619..49260
                     /gene="udk"
                     /locus_tag="ACHURJ_000044"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFR1279149.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHNLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            49352..49933
                     /gene="dcd"
                     /locus_tag="ACHURJ_000045"
     CDS             49352..49933
                     /gene="dcd"
                     /locus_tag="ACHURJ_000045"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFR1279150.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            49955..51808
                     /gene="asmA"
                     /locus_tag="ACHURJ_000046"
     CDS             49955..51808
                     /gene="asmA"
                     /locus_tag="ACHURJ_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFR1279151.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRNYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEVVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIHGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVVTQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALSGAGTLNLAEQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(52082..53665)
                     /locus_tag="ACHURJ_000047"
     CDS             complement(52082..53665)
                     /locus_tag="ACHURJ_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFR1279152.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGELEYEV"
     gene            complement(53866..54015)
                     /locus_tag="ACHURJ_000048"
     CDS             complement(53866..54015)
                     /locus_tag="ACHURJ_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1279153.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            54324..55463
                     /gene="wza"
                     /locus_tag="ACHURJ_000049"
     CDS             54324..55463
                     /gene="wza"
                     /locus_tag="ACHURJ_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFR1279154.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKTYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQVLMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            55469..55912
                     /gene="wzb"
                     /locus_tag="ACHURJ_000050"
     CDS             55469..55912
                     /gene="wzb"
                     /locus_tag="ACHURJ_000050"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFR1279155.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISIAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            55915..58077
                     /gene="wzc"
                     /locus_tag="ACHURJ_000051"
     CDS             55915..58077
                     /gene="wzc"
                     /locus_tag="ACHURJ_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFR1279156.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKELIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAMGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            58255..59094
                     /gene="wcaA"
                     /locus_tag="ACHURJ_000052"
     CDS             58255..59094
                     /gene="wcaA"
                     /locus_tag="ACHURJ_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFR1279157.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            59097..59585
                     /gene="wcaB"
                     /locus_tag="ACHURJ_000053"
     CDS             59097..59585
                     /gene="wcaB"
                     /locus_tag="ACHURJ_000053"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFR1279158.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            59582..60799
                     /gene="wcaC"
                     /locus_tag="ACHURJ_000054"
     CDS             59582..60799
                     /gene="wcaC"
                     /locus_tag="ACHURJ_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFR1279159.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTVVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEDVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            60774..61991
                     /gene="wcaD"
                     /locus_tag="ACHURJ_000055"
     CDS             60774..61991
                     /gene="wcaD"
                     /locus_tag="ACHURJ_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFR1279160.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAVVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            62002..62748
                     /gene="wcaE"
                     /locus_tag="ACHURJ_000056"
     CDS             62002..62748
                     /gene="wcaE"
                     /locus_tag="ACHURJ_000056"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFR1279161.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGITMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKV"
     gene            62764..63312
                     /gene="wcaF"
                     /locus_tag="ACHURJ_000057"
     CDS             62764..63312
                     /gene="wcaF"
                     /locus_tag="ACHURJ_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFR1279162.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKT
                     LPANVVCRGNPAVVIRERVETE"
     gene            63339..64460
                     /gene="gmd"
                     /locus_tag="ACHURJ_000058"
     CDS             63339..64460
                     /gene="gmd"
                     /locus_tag="ACHURJ_000058"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFR1279163.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            64463..65431
                     /gene="fcl"
                     /locus_tag="ACHURJ_000059"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             64463..65431
                     /gene="fcl"
                     /locus_tag="ACHURJ_000059"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFR1279164.1"
                     /translation="MSRQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            65431..65910
                     /gene="gmm"
                     /locus_tag="ACHURJ_000060"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             65431..65910
                     /gene="gmm"
                     /locus_tag="ACHURJ_000060"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFR1279165.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLMPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            65907..67130
                     /gene="wcaI"
                     /locus_tag="ACHURJ_000061"
     CDS             65907..67130
                     /gene="wcaI"
                     /locus_tag="ACHURJ_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFR1279166.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLDLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            67133..68569
                     /gene="cpsB"
                     /locus_tag="ACHURJ_000062"
     CDS             67133..68569
                     /gene="cpsB"
                     /locus_tag="ACHURJ_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFR1279167.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEQAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            68762..70132
                     /gene="cpsG"
                     /locus_tag="ACHURJ_000063"
                     /gene_synonym="manB"
     CDS             68762..70132
                     /gene="cpsG"
                     /locus_tag="ACHURJ_000063"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFR1279168.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALARGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALSVDRTDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            70187..71581
                     /gene="wcaJ"
                     /locus_tag="ACHURJ_000064"
     CDS             70187..71581
                     /gene="wcaJ"
                     /locus_tag="ACHURJ_000064"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFR1279169.1"
                     /translation="MTNLKKRERAKTNASLISMAQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            71583..73061
                     /gene="wzxC"
                     /locus_tag="ACHURJ_000065"
     CDS             71583..73061
                     /gene="wzxC"
                     /locus_tag="ACHURJ_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFR1279170.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGIVVCVAVFLLSDAI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIIAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            73229..74509
                     /gene="wcaK"
                     /locus_tag="ACHURJ_000066"
     CDS             73229..74509
                     /gene="wcaK"
                     /locus_tag="ACHURJ_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFR1279171.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVENGVDTAWLVDHHIEDF
                     TASYAVQHWLDVAAQQKAVAITLRELAPFDKRLGTTQEAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFSTPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLNEAVSRERQTGMRMVQSVLERIGEAK"
     gene            74506..75726
                     /gene="wcaL"
                     /locus_tag="ACHURJ_000067"
     CDS             74506..75726
                     /gene="wcaL"
                     /locus_tag="ACHURJ_000067"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFR1279172.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPQGKVAKLRYRASQTLRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFHADVFIAHFGPAGVTAAKLRELDVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISELWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELTTVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            75737..77131
                     /gene="wcaM"
                     /locus_tag="ACHURJ_000068"
     CDS             75737..77131
                     /gene="wcaM"
                     /locus_tag="ACHURJ_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFR1279173.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPGGLVCDNINTGIFIPAGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLTMSGFGPVTQIYIGGKNKQVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSCVALTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERGE"
     gene            77306..78199
                     /gene="galF"
                     /locus_tag="ACHURJ_000069"
     CDS             77306..78199
                     /gene="galF"
                     /locus_tag="ACHURJ_000069"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFR1279174.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            78571..79656
                     /gene="rfbB"
                     /locus_tag="ACHURJ_000070"
     CDS             78571..79656
                     /gene="rfbB"
                     /locus_tag="ACHURJ_000070"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFR1279175.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDGDKKNSFRFHHISTDEVYGDLPHPDEVNNKEQLPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYIVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKISRELGWKPQETFESGIRKTVEWY
                     LANAKWVENVKSGAYQSWIEQNYEGRQ"
     gene            79656..80555
                     /gene="rfbD"
                     /locus_tag="ACHURJ_000071"
     CDS             79656..80555
                     /gene="rfbD"
                     /locus_tag="ACHURJ_000071"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFR1279176.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPEFAQLLNATSVESIAKAANEVGAWVIHY
                     STDYVFPGTGEIPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGMYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLNAVPATAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            80613..81497
                     /gene="rfbA"
                     /locus_tag="ACHURJ_000072"
     CDS             80613..81497
                     /gene="rfbA"
                     /locus_tag="ACHURJ_000072"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFR1279177.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPTPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVDKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDSEQVKILAEPL
                     KKNAYGQYLLNIINNKIS"
     gene            81540..>81870
                     /locus_tag="ACHURJ_000073"
     CDS             81540..>81870
                     /locus_tag="ACHURJ_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFR1279178.1"
                     /translation="MKLVTSLNKNIFYLAILQGSYFILPLLTFPYLVRVLGPYSFGLL
                     GFCQATMQYLVMVTDYGFNWSATQDVSKSKNDKEKLTTIFWNVFFSKIILALSAIVIF
                     FGITFFIK"
BASE COUNT        20514 a        20094 c        21486 g        19776 t
ORIGIN      
        1 tcttaacaac caaaaataaa ttttattaag gaacatatta tgaaacgttc aattattgct
       61 gccgctgtct tttcttcttt ttttatgagc gctggagtat ttgctgcaga cgttgatacc
      121 ggaacattaa ctattaaggg gaatattgca gaatctccgt gtaaattcga agcgggtggt
      181 gattcagtaa gtattaatat gccgactgta ccaaccagtg tctttgaagg taaagctaaa
      241 tattctacct atgatgatgc agtcggtgta accagcagca tgttaaaaat tagctgcccg
      301 aaagaagttg ctggtgtaaa actctcgttg attaccaacg ataaaataac cggtaacgat
      361 aaggcgatag ccagtagcaa cgataccgtg ggttactatc tctatttagg tgataacagc
      421 gatgtcctgg atgtttctgc accttttaac attgagagtt ataaaacagc ggaaggtcaa
      481 tatgctattc cgtttaaagc aaaatacctg aaactgacag ataactcagt gcaatcaggt
      541 gatgtgttat cttctctggt tatgcgtgtg gcgcaggatt aataaataaa attgtaagtt
      601 tctccaggta ataaacatgg ccgctatccc atggcggcct tttaatttaa gaggcattaa
      661 aatgaaagga ttattatctt tactcatttt ttctatggtc cttcctgcac atgccggaat
      721 tgttatctac gggacgcgca ttatttaccc ggcagaaaat aaagaagtga tggtgcagtt
      781 gatgaaccag ggaaaccgtt cttcgctgct gcaggcgtgg attgatgatg gcgatacgtc
      841 attaccacca gaaaaaattc aggttccttt catgttaacg ccaccagtgg caaaaatagg
      901 ggcaaattcc gggcagcaag taaaaatcaa aattatgccg aataaactgc ccactaataa
      961 agaaagcatt ttttatctga atgttctgga tattccacca aatagtccag agcaagaagg
     1021 taagaatgca ctgaagtttg cgatgcaaaa cagaattaag ttgttttacc ggccagcggg
     1081 tattgctccg gtaaataaag cgacatttaa aaaattgctg gtaaatcgca gtggcaatgg
     1141 tttggtgata aaaaatgact cagctaattg ggtgacgatt tcggatgtca aagctaataa
     1201 tgtcaaagtc aattatgaaa ctattatgat tgccccctta gaaagtcaga gtgttaatgt
     1261 caaaagtaat aatgcaaata actggcatct gaccattatc gatgaccatg gcaactatat
     1321 tagtgacaaa atttaattgc aggagctgcc tatgttgaga atgaccccac ttgcatcagc
     1381 aatcgtagcg ttattgctcg gcattgaagc ttatgcagct gaagaaacct ttgataccca
     1441 ttttatgata ggtggaatga aagaccagca ggttgcaaat attcgtcttg atgataatca
     1501 acccttaccg gggcagtatg acatcgatat ttatgtcaat aagcaatggc gcgggaaata
     1561 tgagattatt gttaaagaca acccgcaaga aacatgttta tcaagagaag ttatcaagcg
     1621 gttaggcatt aatagcgata acttcgccag cggtaagcaa tgtttaacat ttgagcaact
     1681 tgttcagggt gggagctata cctgggatat cggggttttt cgtctcgatt tcagtgtccc
     1741 gcaggcctgg gtggaagaac tggaaagtgg ctatgttcca ccggaaaact gggagcgggg
     1801 tattaatgcg ttttatacct cttattatct gagtcagtat tacagcgact ataaagcgtc
     1861 gggtaataac aagagtacat atgtacgttt taacagcggg ttaaatttac tggggtggca
     1921 actgcattct gatgccagtt tcagtaaaac aaataacaat ccaggggggt ggaaaagcaa
     1981 taccctgtat ctggaacgtg gatttgccca acttctcggc acgcttcgcg tgggtgatat
     2041 gtacacatca agcgatattt ttgattctgt tcgcttcaga ggtgtgcggt tgtttcgtga
     2101 tatgcagatg ttgcctaact cgaaacaaaa ttttacgcca cgggtgcagg ggattgctca
     2161 gagtaacgcg ctggtaacta ttgaacagaa tggttttgtc gtttatcaga aagaggttcc
     2221 tcctggcccg ttcgcgatta cagatttgca gttggccggt ggtggagcag atcttgatgt
     2281 cagcgtgaaa gaggcggacg gttcggtaac cacctatctg gtgccttatg cagcggtgcc
     2341 aaatatgctg caacccggcg tgtcgaaata tgattttgcg gcgggtcgta gccatattga
     2401 aggggcgagc aaacaaagtg aatttgtcca ggtgggttat cagtatggtt ttaataattt
     2461 attgacgctg tatggcggct cgatggtcgc gaataattat tacgcgttta ctttggggac
     2521 tggctggaat acacgcattg gtgccatttc cgtcgatgcc actaagtcgc atagtaaaca
     2581 agacaacggc gatgtgtttg acgggcaaag ttatcaaatt gcctacaaca aatttgtgag
     2641 ccaaacgtcg acgcgttttg gtcttgcggc ctggcgttat tcatcgcgtg attaccggac
     2701 atttaatgat cacgtttggg caaacaataa agataattat cgccgtgatg aaaacgatgt
     2761 ctatgacatt gccgattatt accagaacga ttttggccgt aaaaatagct tttctgccaa
     2821 tatgagccag tcattgccag aaggctgggg ttctgtgtcc ttaagtacgt tatggcgaga
     2881 ttactgggga cgtagcggca gcagtaagga ttatcagttg agttattcca ataactggcg
     2941 gcggataagc tataccctcg cggcaagcca ggcttatgac gagaatcatc atgaagagaa
     3001 acgatttaat atttttatat cgattccctt tgattggggt gatgacgtta cgacgcctcg
     3061 tcggcaaata tatatgtcta actcaacgac gtttgatgat caggggtttg cctcaaataa
     3121 tacgggatta tcaggaacag tagggagtcg ggatcagttc aattatggtg tcaacctgag
     3181 tcatcaacat cagggaaatg aaacgacagc tggggcgaat ttgacctgga acgcgccggt
     3241 tgcgacagtg aatggcagtt atagtcagtc gagtacttat cgacaggctg gagccagtgt
     3301 ttcagggggc attgtcgcct ggtcgggtgg cgttaatctg gcgaaccgtc tttccgaaac
     3361 gtttgctgtg atgaatgcgc caggaattaa agatgcttat gtcaatgggc aaaaatatcg
     3421 cacaacaaac cgtaatggag tggtgatata cgacggaatg acaccttatc gggaaaatca
     3481 cctgatgctg gatgtgtcgc aaagcgatag cgaagcagaa ttacgtggca accggaaaat
     3541 tgccgcccct tatcgcggcg cggttgtact ggttaatttt gataccgatc agcgcaagcc
     3601 ctggtttata aaagcgttaa gagcggatgg gcaaccatta atgtttggtt atgaagtcaa
     3661 tgatatccat ggtcataata ttggcgttgt cggtcaggga agccagttat ttattcgcac
     3721 caatgaagta ccgccatcgg ttaatgtagc aattgataag caacaaggac tttcatgcac
     3781 aatcaccttc ggtaaagaga ttgatgaaag tagaaattat atttgccagt aatagtcagg
     3841 tggttctatg gaaattcgca taatgctatt tatattaatg atgatggtta tgcctgtgag
     3901 ctatgcggca tgttatagtg agttatctgt tcagcacaac ttggttgttc agggggattt
     3961 tgcacttact caaacacaaa tggcgacata tgagcataat tttaatgatt cgtcatgcgt
     4021 gagtacaaat actatcaccc ctatgagccc gtcggatatt attgttggac tttataacga
     4081 taccattaaa ttaaatttac attttgaatg gaccaataaa aataacatca cgttgtcaaa
     4141 taatcagacc agtttcacca atggttattc ggtaacggtg acacctgcgg ccagtaatgc
     4201 aaaagtgaat gtttctgcgg ggggcggcgg ttcagtgatg attaatggtg ttgcgacatt
     4261 atccagtgct tcatcatcga cacgcgggag tgccgcagta caatttctac tgtgtttatt
     4321 aggtggcaag tcatgggatg catgtgtaaa tagctataga aatgcattgg cacaaaatgc
     4381 aggtgtctat tcctttaatc tgaccttgtc atacaacccg ataaccacaa cctgcaaacc
     4441 ggacgattta ttaattactt tagagggtat tcccgtttca caattaccag ccacaggtaa
     4501 caaagcaaca ataaatagta aaaaagggga tattattctg cgttgtaaaa atttattagg
     4561 tcaacaaaat caaacatcac ggaaaatgca ggtgtattta tcaagttctg acttgttaac
     4621 caacagcaac acaatactga aaggtgcgga agataatggc gtaggattta ttcttgaaag
     4681 taatggttcg ccagtcacac ttttaaatat cactgataac agcaaaggat acacaaattt
     4741 aaaagaaatt gcggcaaagt caaaacttac agatacaacg gtttcaattc cgataacagc
     4801 cagttactac gtctacgata caaacaaagt taaatctggc gcattggagg caaccgcatt
     4861 aatcaacgtg aaatacgact aataacaagc aagacgagca agtggctaat aataaaaaat
     4921 aacaaggtgt ctgcatttcc ccctcatgat gagggggatt tttttagcga tgataaaaaa
     4981 tctcaccgtc gtaggcttta atgattttac cgtcggtgtc gctgatcaac acgtacgcgc
     5041 cacccatata ggtccagtgc gtcccggcat caggcgcggg caggttacgc aactgccact
     5101 gcttaatgtt gtactcatcg gtacggtaca tttctggtac ggtgtcccct ggtttgaaat
     5161 gcttaaagtc agcaatgaaa ctacttaact catacttaga aattcccgag gtattggtcg
     5221 aacccgcggt ggctggtgcg gcccaggcgg cactggtaac cagcaaaagc gcacccagca
     5281 acatcttatt tttaatggtc ataaactctc cactttgggg atctgtaata tcagtcgcat
     5341 atctttcctg ccatacaata taactgacaa gcgatatttt gttagtgatc ccatgatttc
     5401 gtaaaaagtg taaatgaaag gatgccctaa aagcatcctt agcgagcaga gagtcagcgg
     5461 aaatagctga tttaagaatc atcctgggct gaagataaca tgcaagccta aaggattgag
     5521 ttatcgcatt atgcccatga agccatgtac gcccatatac acaccgacta agccaatcaa
     5581 cagactggaa aaataggggg cgcgtttagc gagagtgtta aatccgctcc agcgttttgc
     5641 gacctgctga acgctgattg ctgcgccaac gcctacggtg acaagcgtta acgccaggcc
     5701 aatgctgaaa ctgacgacca gtgttgcgcc cagtgtcagg gctttcaact gaatgcatat
     5761 caacagcacg gtaattgctg ccgggcaggg gataaggcca ccggttaagc caaataacaa
     5821 aatttgccag ttggtgacct ctctaccatc aaagcgtcgt ttaatgtcat tggcatgggc
     5881 tcgtgcatgg gcatcctgat actcgccatg ttcgtgatgg tgatgatgtc catggtcgtg
     5941 gtgatgttcg tgatcgtgat gatgatgctc ataatcatgc ccgtgcatat tctccagcca
     6001 gttgcgttcg ccgcgccagg tacgccagaa catccagaac gcggtgctaa taatgatcac
     6061 tgcggaaatc agctggagcc acggttctgc tgattgagca gtaaagcgct tgctgatcac
     6121 catcccgcca aaggcaatta accagaccac tgcggtatgc gaaatagttg ctgccagtcc
     6181 gagcatcacc gcttgtttaa tggtgccttt gatggcgatg ataaacgccg ccatcatcgt
     6241 ttttgagtgc cctggttcca ggccatgaag cgcaccaagt aagatggcgc tggggatgaa
     6301 gaaccaggcg tttccttgct gaagaagagt tgtaaattcg gtcatgataa taattcttag
     6361 tattaattcg gcaatctgat tctactcccc cccagtacct gatactaccc cccagtagat
     6421 taatagtgct atgatttttc atattcttgt taaccaggtg ttgccatgtc tcatacaatc
     6481 cgtgataaac agaaactgaa agcgcgtgcc agtaagattc agggccaggt cgtggcgctc
     6541 aagaaaatgc tcgacgagcc gcacgaatgc gctgcagttt tacaacagat tgctgctatc
     6601 cgtggcgcgg taaacggtct gatgcgggaa gtgattaaag gtcatctgac ggaacacatc
     6661 gttcaccagg gggatgagct aaaacgtgaa gaagatctgg atgtcgttct gaaggtgctg
     6721 gattcatata tcaaataatt tattaacgcg attgtaaaac tgccgttttt cctcgtttac
     6781 aacgcgtgcg ctggacatta ccatcctcct ctgcgattta tcatcgcaac caaacgactc
     6841 ggggtgccct tctgcgtgaa ggctgagaaa tacccgtatc acctgatctg gataatgcca
     6901 gcgtagggaa gtcacggacc accaggtcat tgcttcttca cgttatggca ggagcaaact
     6961 atgcaagtcg acctgctggg ttcagcgcaa tctgcgcacg cgttacacct ttttcaccaa
     7021 cattcccctc ttgtgcactg catgaccaat gatgtggtgc aaacctttac cgccaatacc
     7081 ttgctggcgc tcggtgcatc gccagcgatg gttatcgaaa ccgaagaggc cagtcagttt
     7141 gcggctatcg ccagtgcctt gttgattaac gttggcacac tgacgcagcc acgcgctcag
     7201 gcgatgcgtg ctgccgttga gcaagcaaaa agctctcaaa caccctggac gcttgatcca
     7261 gtagcggtgg gtgcgctcga ttatcgccgc catttttgtc atgaactttt atcttttaaa
     7321 ccggcagcga tacgtggtaa tgcttcggaa atcatggcat tagctggcat tgctaatggc
     7381 ggacggggag tggataccac tgacgccgca gctaacgcga tacccgctgc acaaacactg
     7441 gcacgggaaa ctggcgcaat cgtcgtggtc actggcgaga tggattatgt taccgatgga
     7501 catcgtatca ttggtattca cggtggtgat ccgttaatga ccaaagtggt aggaactggc
     7561 tgtgcattat cggcggttgt cgctgcctgc tgtgcgttac caggcgatac gctggaaaat
     7621 gtcgcatctg cctgtcactg gatgaaacaa gccggagaac gcgcagtcgc cagaagcgag
     7681 gggccaggca gttttgttcc acatttcctt gatgcgctct ggcaattgac gcaggaggtg
     7741 caggcatgaa acgaattaac gctctgacga ttgccggtac tgatccgagt ggtggtgcgg
     7801 ggattcaggc cgatcttaaa accttctcgg cacttggcgc ttatggttgc tcagttatta
     7861 ctgcactggt ggcgcaaaat acccgtggcg tacagtcggt gtatcgcatt gagcctgatt
     7921 ttgtcgccgc ccagctcgat tcggtgttca gcgatgtgcg aatcgatacc actaaaatcg
     7981 gtatgctggc ggaaaccgat attgttgaag cggtggcaga acggttgcaa cgttatcaga
     8041 tccaaaacgt ggtactcgac accgttatgc tggcaaaaag cggcgacccg ctgctttcac
     8101 cttcggcggt tgctacgctg cgcagtcgat tattgccaca ggtttcatta ataacgccaa
     8161 acttgcccga agctgccgcc ttgctcgacg cgccacacgc gcgcaccgaa caggaaatgc
     8221 tggaacaagg gcgatcgctg ttggcgatgg gctgtggcgc agtgctaatg aaaggtggtc
     8281 atctggatga tgagcaaagc ccggactggc tgtttacccg cgagggtgaa caacggttta
     8341 ccgcaccgcg cattatgacc aaaaacaccc acggcactgg ttgtacactc tctgcggcgt
     8401 tggctgcact acgcccgcgc catacaaact gggctgacac cgtacaggag gcaaaaagct
     8461 ggctttcatc ggcgttagcc caggccgaca cgctggaagt tggtcacggt attggtccgg
     8521 ttcaccactt ccacgcctgg tggtgagcac cgtactatag ctgctttggt tattgtcagc
     8581 ctttggcact gacattcagg gatgaaacca atgcagctaa gaattaccag tcgaaaaaaa
     8641 ctcacctctc tattatgcgc tcttgggcta atttccatcg ttgcaattta tccgcgtcag
     8701 acggtgaatt ttttctattc gacagcagtt cagattacag actacatcca cttctacggt
     8761 tatcgcccgg ttaaatcttt cgctattcgc attcctgcca gttacaccat tcacggaata
     8821 gatgtttcgc gctggcagga gcggatcgac tggcagcggg tggcaaaaat gcgcgacaac
     8881 ggtatccgct tacagtttgc ttttattaag gcgacggaag gcgaaaagct ggtggacccc
     8941 tatttttcgc gtaactggca actaagccgc gaaaatggcc tgctgcgcgg ggcatatcat
     9001 tatttctccc cgtcggtagc agcaccggtt caggtgcgat tatttctgca aacggtagat
     9061 ttctcacaag gcgatttccc tgcggtactg gacgtagaag agcgaggaaa attatcggca
     9121 aaagaattac gcaagagggt aagtcagtgg ctaaaaatgg tcgaaaaaag tacggggaaa
     9181 aagccgatta tttactcagg agccgttttt tatcacacga atctggcggg ctatttcaat
     9241 gaatatccgt ggtgggtggc tcactattat caacgtcgtc cggacaatga cggcatggcc
     9301 tggcgcttct ggcagcattc cgaccgtgga caggtagatg gcatcaatgg tccggtggat
     9361 tttaatgtat ttaatggcac ggtggaggag ttgcaggcat tcgttgatgg gattaaagaa
     9421 acgccttaaa taagaagcag tgatatactc ccaccagact ccccggcagg aaaaagacaa
     9481 atggaacaag cgcataccca gctgattgcc caactgaacg agcgtatttt agcggcggat
     9541 aacacgccgc tttatattaa gtttgccgaa acggtaaaaa atgccgtgcg cagcggggtg
     9601 ctggagcatg gcaatatttt gcccggtgag cgtgatttaa gtcagttaac cggcgtttcg
     9661 cgcattacgg tgcgcaaggc gatgcaggcg ctggaagaag agggtgtggt gacgcgttcg
     9721 cgcggttacg gcacgcagat caacaacatc ttcgaatact cgttgaaaga agcgcgaggt
     9781 ttttctcagc aggtggtatt gcgcgggaaa aagcccgata cgctatgggt taacaagcgt
     9841 gtcgtgaaat gtcccgaaga agtcgcgcag cagctggcgg tcgaagcagg aagtgatgtc
     9901 tttttgctta agcgtattcg ctatgtcgat gaagaagcgg tatcgattga ggaatcgtgg
     9961 gttccggcgc atttaattca tgatgttgat gccatcggga tttcacttta tgactatttc
    10021 cgcagccagc acatttaccc acaacgtacg cgttcccgcg ttagcgcccg gatgccggat
    10081 gccgagtttc agtcacatat tcagttagat agcaaaatac cggtgctggt gatcaagcaa
    10141 gtggcgcttg accaacagca acggcctatt gagtacagca ttagccactg tcgcagcgat
    10201 ctatacgttt ttgtgtgcga ggagtagttc ctcgcgcgtt ggcgcacaat caccgccccg
    10261 atgcccgaca acccacgacg ccactgcatt gcccagtaat acggcatccg ccagtggcag
    10321 accagaggcc agcccggcaa gtacgccacc ggcatgactg tcgcccgccc caatggtgtc
    10381 gacaacttgc gttggaaatg ccggaatgca gccagaagcg tcgttgctga aataccatgc
    10441 gccttcttta tcgagacgaa cgatcaacgg cgcggcaaat ttctcctgcc attgcttgcc
    10501 aagtgttgtt atctctgcgg ataaagcaaa acgttcggcg gcaatctcag cctcttgacg
    10561 attgagcgac actaaaggtc gacaggccat gatccgcgcc agtaatgcat ccgggatatc
    10621 gccaatacgt gggccaaaat cgataaacgg cgtcacgtct tgcagctctt ccagccattc
    10681 cactaacaat tcgccgcagg gcgaggccag ttgataaccg gaaaaataga gcaggctgcc
    10741 aggcgcaacg gttaatcgcg ccagccactg gcgattccac tgattttcca caccgctgaa
    10801 tgacataaaa gtgcgttcgc catccggctc aaccagcgcc agacaccagc cgttatcacc
    10861 ttcggcgtta tcgatcaggc tgattaagcc ctcttttgcc atccggttgc gaataatctc
    10921 cgcccacacg ccctgaccga gcggcaaggc attacccgct tcgatgccga ggcgctttaa
    10981 cgccacggca atattcagtg cgcagccgcc aacgttaacg ctctgctgtt tcagttcgat
    11041 atcgcaccca cgccagggga gggcataagc gtcggcgatc acgtcaatga ccgccgcgcc
    11101 gacgaccatc accggctgac gcgtggttaa ttcaggcagc agcgtgtgta atcgagcgcc
    11161 gctcataccg cctcccgttg ttgacgatat ttcgccagcg ctgtggcata gcggttgaaa
    11221 tcaagctgat ttaccgcatc cagttccgcc tttaacgcag gatcgatagc gttaacgcca
    11281 tgcaacgcgc cgcaaattgc cgtcgccata gcaccgatgg tgtctgtgtc gccgccaagg
    11341 ttagcgcaca ggacggcgca gcgattcggg tcggtttgtg ccagttcaac cagcgcaatg
    11401 gcgcacggaa cggactcaat agtgctggta cctgcgccaa cgacctggta aagctgttcg
    11461 ctggcggatt cggtgccgtc ggcattgcgc acaattttca gcgcaatctc cagccgcgcc
    11521 gccagtgagg cgctgaacgt ggtggtgcgt ttttgctggg catgtcgcgc aattgaaggc
    11581 agtgaatcaa caatcgctga ccagctttct ccgtcaatgg cacgagaaat cgcccatgcg
    11641 atgactaccg cgcctgcaac cgccagatcg gatttatggg tcgggctgga cgccagcgct
    11701 acatcatcaa taaaggaatc aacatcacgc gccggaagca aacaacctaa tggcgagacg
    11761 cgcatcgctg cgccgttggt cacgccgttg ttttccagtt cagcaacggg tttaccgtcg
    11821 cgaatggcgt taagcgcaat cttcgaggtc ggacctagta cgtttttgtt aaaggcgtcg
    11881 aaacgcagcg cccagtcgag aatattacgc ccaatcagat ccggatcgat cttgccttca
    11941 cgttccagta acgcatccgc cagacacagc gccatcgagg tatcgtcggt gaattcggcg
    12001 cggttaaaat aacaggccgc gttattctcc tttggtccag gaagaaaacg gtcaatccag
    12061 ccaaagtgtg ctttaacgcg gctgcgtggc caaagctcgg agggcatccc catcgcatcc
    12121 cctaacgcct gcccataaag agcaccgaga atacgttctg ttttcattta acttcccctt
    12181 gtgtcaacgc aatatcgcga tcatcgacct tgatagccgt aatttcgttg tcggattcgc
    12241 ggaaaaaaat catgaacagc acggcgataa tggcaatcat caccgcgcca aaagtccaca
    12301 tcccggacca gttgaaagtc agtccgttta ccggttcctg ataagcgaac atcttttcca
    12361 tcatcacacc gccaagacga tagccgagca aactgccgaa gccctggcag cagagcgtga
    12421 tcagcccctg cgcggcggta cgcatatgca cgggggcttt tttatcgaca tagatgtaag
    12481 cggtaacgta gtaaaaatcg taacttacgc cgtgaagcaa aataccgagg aacagtaacg
    12541 cgtaggtgaa atattcatcc gcactaccgt aaataaagaa gccatagcgg atcgcagcgg
    12601 tgaccagacc aagcaataat acctttttga taccaaagcg tttagtgaaa aacggtaatg
    12661 ccagcataaa gaatatttca gagaactggc cgagcgtcat ccagccggtg gcatttttca
    12721 tgccgacttc ggtcagataa ccgttggcaa agatgtaata gaacgccagt ggcatcgcaa
    12781 acaggaatga acagaagaaa aagacgagga agtttttatc gcgcagcagg atcagcgcat
    12841 ccaggccgag catgacttta atatccattt tgccggtgct ttttggtggc gtgtcgggca
    12901 ggaaaaacgc aaacacaccg agcagagcag aacttccggc ggtaatcagc agcgggatgt
    12961 tagtcggtga gatatcggca taccccagta tttgcggcaa gaaaccacat gccagaccgg
    13021 aggcaatcca gccgatagtg ctcatcacac gaatgcgcgg gaaatcacgc tccacatccg
    13081 gcacgttggc aaaagcgatg ctgttagtca gcgcaatggt cggcatatag gttagagagt
    13141 aagccagcag taacggaaag aacccggcaa aagtggtctg ttgcgcagcg aaatacatca
    13201 gcagcgcgcc tgcgaacatc aataccgcca gcactttttg cgccgagaaa aagcggtcag
    13261 tgatggagcc aaccagaatt ggcgacagga tcgccgcaat ggcggtacag gcatacgacc
    13321 agccaatttc tccggcacta aaaccgcttt tacttaacca caaccacaat ggcacaaacc
    13381 acgcgcccca gataaaccat tcaacaaaca tcatgaacga cagctttgct gttgttttca
    13441 tcttttaatt ccttcatgac aggtaatgta aggtacgcga tgacaatacc acttaataat
    13501 accttttaaa tacctttacg gtgaaaactt gaccgcgcta acattttttc tgatgaatcg
    13561 agccaacaga aaacgctgaa aaaacatcca aaagatggaa aaactcgccc gtaacgacca
    13621 ggattatgat tgcccgcaaa aggcgggcca ggacaatcca tagccgatat ccaatcggaa
    13681 tttacgggag catagtaatg acagatattg cgcagttgct tggcaaagac gccgacaacc
    13741 ttttacagca ccgttgtatg acaattcctt ctgaccagct ttatctcccc ggacatgact
    13801 acgtagaccg cgtaatgatt gacaataatc gcccgccagc ggtgttacgt aatatgcaga
    13861 cgttgtacaa caccgggcgt ctggctggca caggatatct ttctattctg ccggttgacc
    13921 agggcgttga gcactctgcc ggagcttcat ttgctgctaa cccgctctac tttgacccga
    13981 aaaacattgt tgaactggcg atcgaagcgg gctgtaactg tgtggtatca acttacggcg
    14041 tgttggcgtc ggtatcgcgg cgctatgcgc atcgcattcc atttctcgtc aaacttaatc
    14101 acaacgagac gctaagttac ccgaatacct acgatcaaac gctgtatgcc agcgtggagc
    14161 aggctttcaa catgggcgcg gtggcggttg gtgcgactat ctattttggc tcggaagagt
    14221 cacgtcgcca gattgaagaa atttctgcgg cttttgaacg tgcgcatgag ctgggcatgg
    14281 tgaccgtgct gtgggcctat ttgcgtaact ccgcctttaa gaaagatggc gttgattacc
    14341 atgtttccgc cgacctgacc ggtcaggcaa accatctggc ggcaaccatc ggcgcagata
    14401 tcgtcaaaca aaaaatggcg gaaaataacg gcggctataa agcgattaat tacggttata
    14461 ccgacgatcg cgtgtacagc aagttaacca gcgaaaaccc gattgatctg gtgcgttatc
    14521 agttagctaa ctgctatatg ggccgggctg ggttgataaa ctccggcggt gctgcaggcg
    14581 gtgaaactga cctcagtgat gcagtgcgta ctgcggttat caacaaacgc gctggcggaa
    14641 tggggctgat tcttggacgt aaggcgttca agaaatcgat ggctgacggc gtgaaactga
    14701 ttaacgccgt gcaggacgtt tatctcgata gcaaaattac tattgcctga tacgccttat
    14761 acgaacaaca ccaccccgta ggacggataa ggcgtttaca ctgcatccgg caacctatgc
    14821 ctgatgcgac gcggaagcgt cttatcatgc ctacatagca ctgccacgta tgtttacacc
    14881 gcatccggca taaaaacacg cgcactttgc tacggcttcc ctatcgggag gccgtttttt
    14941 tgcctttcac tcctcgaata attttcatat tgtcgttttt gtgatcgtta tctcgatatt
    15001 taaaaacaaa taatttcatt atattttgaa aacgaaaaca aacgacagga tatgaaaatg
    15061 cacgtggtat cgacaaagca gatgctgaac aacgcacagc gcggcggtta tgcggttccg
    15121 gcattcaata ttcacaatct cgaaacgatg caagtggtgg tggaaaccgc tgccaacctg
    15181 catgcgccgg tcatcatcgc cggaacgcct ggcacattta ctcatgctgg tacagaaaat
    15241 ctgttggcgc tggtcagcgc gatggcgaag caatatcacc atccactggc aattcatctc
    15301 gaccatcaca cgaaatttga cgatatcgct cagaaggttc gttctggcgt gcgctcagtc
    15361 atgattgacg cctcgcattt gccttttgcg caaaatattt cacgggtcaa agaggtggtg
    15421 gatttttgcc atcgctttga tgtcagcgtc gaagcggagc tggggcaact tggcggccag
    15481 gaagatgatg tgcaagtcaa tgaagccgat gctttttata ccaaccccgc tcaggcgcgt
    15541 gaatttgccg aggcaaccgg aattgattcc ctggcggtcg ccatcggcac ggctcatggg
    15601 atgtatgcca gcgcaccggc gcttgatttt tctagactgg agaacattcg ccagtgggtg
    15661 aacttaccgc tggtgctgca tggcgcgtca gggttatcga ctaaggatat tcagcaaacc
    15721 atcaaactgg ggatatgcaa aatcaacgtt gcaacggagc tgaaaaatgc cttctcgcag
    15781 gcgttaaaaa attacctgac cgagcaccct gaagcgaccg agccccggga ttatttgcag
    15841 tcggctaaat ccgcaatgcg cgatgtggtg agcaaagtga ttgccgattg tggctgcgag
    15901 ggcagggcat aacgcacctg ccatttaata aggaaaaaac atgaaaacgt taattgcccg
    15961 gcataaagct ggtgaacata tcggcatatg ttcagtctgt tctgcccatc cgttggttat
    16021 cgaagcggcg ctggcatttg atcgcaacag cacgcgcaaa gtgctgattg aagcaacgtc
    16081 aaaccaggtc aatcaatttg gcggttatac cggaatgaca ccggcagact ttcgcgaatt
    16141 tgtttttacg attgccgata aagttgggtt tgcacgcgaa cgcattattc tcggcggcga
    16201 tcatctgggg ccaaactgct ggcagcaaga aaatgcggat gcggcgatgg aaaaatccgt
    16261 cgagctggta aaggaatatg ttcgtgccgg cttcagtaaa attcatcttg atgcgtcaat
    16321 gtcctgcgcg ggggatccca taccgttagc accagaaacg gttgcggaac gagctgctgt
    16381 gctttgcttt gctgcggaaa gtgtggcgac agattgccag cgtgagcaac tgagctatgt
    16441 cattggcacc gaagttccgg ttccgggcgg tgaggccagc gccattcagt cagtacacat
    16501 cacccatgtt gaagatgccg ccaatacttt acgtacgcat caaaaggcct ttattgtccg
    16561 tgggctgaca gaggcgttaa cacgcgtgat tgccatcgtg gtgcagccgg gcgtggaatt
    16621 tgatcacagc aatattatcc attatcagcc gcaggaagcg cagccgctgg cgcaatggat
    16681 agaaaacacc cgaatggttt atgaagcaca ttctaccgat taccagaccc ggacggctta
    16741 ttgggaatta gtccgcgatc actttgcaat attgaaagtc ggtcccgcat taacctttgc
    16801 tttacgcgag gcgatatttg cactggcaca aattgagcag gaacttatcg cccctgaaaa
    16861 tcgcagcggt tgcctggcgg taattgaaga agtgatgctc gacgaaccgc aatactggaa
    16921 aaaatattat cgtacgggtt ttaacgattc attactggat attcgttaca gcctgtcgga
    16981 tcgtattcgt tattactggc cgcatagtcg gattaaaaat agcgtcgaaa cgatgatggt
    17041 gaatcttgaa ggcgtggaca tcccactggg catgattagt cagtatcttc ccaaacaatt
    17101 tgaacgcatt cagtccgggg aattatcagc aataccgcat tagctgatta tggataaaat
    17161 ttatgatgtt ttgcgcgcct atcgctacgg ctgtgcggaa taaggacggt atatgactaa
    17221 cctgtttgtt cgtagcggaa tttcttttgt cgatcgtagc gaagttttaa cccatatcgg
    17281 taatgagatg ctcgccaaag gtgtggttca tgatacctgg ccacaggcat taattgccag
    17341 agaagcagaa ttccctaccg ggataatgct tgagcagcac gccattgcaa taccgcattg
    17401 tgaggcgatt catgctaagt cgtcagccat ttatctgtta aggccaacaa ataaagttca
    17461 ttttcagcaa gcggatgatg ataacgacgt ggaggtatcg ttggttattg cgttgattgt
    17521 ggaaaatccg cagcagcaat tgaaactttt acgctgttta tttggcaagt tacaacagcc
    17581 cgatatcgtc gagacactaa tcactcttcc tgaaacccag ttaaaggaat acttcacaaa
    17641 gtatgtttta gattcagacg aataaatccc tctgtaacaa taaaaaggac tatttatgaa
    17701 acgcaagatt attgtcgctt gcggaggcgc ggttgcgacc tctacgatgg cggcggaaga
    17761 aattaaagaa ttgtgtcaga atcataatat tcctgttgaa ttaatccagt gtcgggttaa
    17821 tgaaatagaa acctatatgg atggcgtgca tttgatatgc actactgcca gggtggatcg
    17881 tagttttggc gatattccgt tagttcacgg catgcctttt gtttctggtg tcggtatcga
    17941 agcattacaa aataaaattc tgactatctt acaggggtga cctatgtttt cagaagtcat
    18001 gcgttatatt ctcgacctcg gccctacggt gatgctgccg attgtcatca ttattttttc
    18061 taaaatatta ggcatgaagg caggcgattg ctttaaagcg ggtcttcata tagggattgg
    18121 ctttgttggc attggccttg tgattggctt aatgctggat tccattggtc cggcggcgaa
    18181 agcgatggcg gaaaatttcg acctgaatct gcatgtggtc gatgtcggct ggccgggctc
    18241 ttcaccaatg acctgggcgt cgcaaattgc gctggtggcg attccgattg ctattctggt
    18301 taacgtggcg atgttactga cccgtatgac gcgggtggta aatgttgata tctggaatat
    18361 ctggcatatg actttcaccg gcgcgttgct gcatctggca accggttcat ggatgatagg
    18421 gatggcgggt gtggtaattc acgcggcgtt tgtttataag ctcggcgact ggtttgcccg
    18481 cgatacccga aatttctttg agctggaagg tattgctatt ccgcacggta cgtcggcgta
    18541 tatggggccg attgcggtgc tggtcgatgc tatcatcgag aaaatcccag gcgttaaccg
    18601 aattaaattt agcgccgatg atattcagcg caaatttggc ccttttggcg aacctgtcac
    18661 cgtgggtttt gtgatggggc tgattatcgg catcctcgcg ggttacgatg tcaaaggtgt
    18721 attgcagctg gcggtaaaaa cggcggcagt gatgctgcta atgccacggg tgattaaacc
    18781 catcatggat ggtttaacgc ccatcgctaa gcaggcccgt agtcgtttac aggcgaagtt
    18841 cggcggtcag gagttcctga ttggcctgga tcccgcatta ctgctggggc atacggcggt
    18901 ggtatcggta agcctgattt ttatcccgct caccatttta attgctgttt gtgttccggg
    18961 taatcaggta ctgccgtttg gcgatcttgc caccatcggc ttctttgtgg cgatggcagt
    19021 cgccgtgcat cgtggaaatc tgttccgcac cttaatctcg ggtgtcatca tcatgagcat
    19081 cactctgtgg atcgcgacgc aaactattgg tttgcacacc caactggcgg ctaatgctgg
    19141 ggcgttaaaa gccgggggta tggtggcttc aatggatcag ggcggttctc ccattacctg
    19201 gttactgatt caggttttct ccccgcaaaa tattcccggt ttcattatta tcggcgcaat
    19261 ttatctgacc ggtattttca ttacctggcg tagagcgcgt ggttttatta aacaagagaa
    19321 agccgttctc gcagaataat ttttaactga gggggagttt atccccctta acaatcagga
    19381 gtttttatga aatcagtggt gaatgatact gatggtatcg tgcgcgttgc agaaagcgtc
    19441 attcctgaaa ttaaacatca ggatgaggtg cgggtaaaaa ttgccagctc gggcttatgt
    19501 ggttccgatt tacccaggat atttaaaaat ggtgcacatt attatccaat aacgttaggc
    19561 catgaattta gcggctatat tgatgcggtg ggatccggtg ttgatgattt acaccctggc
    19621 gatgcggttg cctgtgtgcc gttattaccc tgttttactt gtccagagtg tctgaaaggg
    19681 ttttattccc agtgcgcaaa atatgatttt attggctcgc ggcgtgatgg tggatttgct
    19741 gaatatattg tcgttaagcg aaaaaatgtc tttgctctac ccacggatat gcctattgag
    19801 gatggggctt ttattgagcc gattaccgtt ggtctgcatg cttttcattt agcgcaaggt
    19861 tgtgagaata aaaacgttat tattattggt gccggaacca ttggcctgct ggctattcag
    19921 tgcgctgtcg cgctgggagc aaagagtgtg acggctatcg acattagctc agaaaaactg
    19981 gcactggcaa aatctttcgg ttcgatgcaa acatttaaca gtagcgaaat gagcgcgccg
    20041 caaatgcaga gcgttttacg cgaactgcgt tttaatcagc ttatcctcga gacggctggc
    20101 gtaccgcaaa ctgtcgaact ggcggtagag attgccggtc ctcatgccca actggcgctg
    20161 gtgggcacgt tgcatcagga tctgcattta acatcggcaa cgtttggcaa aatattgcgt
    20221 aaagagctga cggttatcgg cagttggatg aactattcca gcccttggcc ggggcaggag
    20281 tgggaaacgg cgagccggtt gctgacagaa cgtaagttaa gcctggagcc attaatcgct
    20341 caccgtggaa gctttgaaag cttcacccag gcggtgcgtg acatcgctcg taatgccatg
    20401 ccgggcaaag tgttgctcat tccatgaaac cgcgggccag cgtgatgctg gcccggtatt
    20461 gtgcaaaaca gatcattcac caatggtccc ccttcgttta cactagccac aattgaatat
    20521 gggtaaatga cgatgaattc attcgagcga aggaataaga tcatccaatt agtgaatgaa
    20581 cagggaaccg tgcttgttca ggatctggcg ggagtatttg ctgcctcgga agcgacaatc
    20641 cgtgccgatt tgcgctttct cgaacaaaaa ggcgtggtta cgcgctttca tggcggtgcg
    20701 gcgaaaataa tgtccggtaa tagtgaaacc gagacccagg aagtcgggtt taaagagcga
    20761 tttcagctcg ccagcgcgcc aaaaaacaga atagcgcagg cggcagtcaa aatgatccat
    20821 gaagggatga ccgttattct cgacagcgga agtaccacaa tgcttatcgc cgaaggatta
    20881 atgaccgcta aaaatatcac ggtgattacc aacagtctcc cggcagcgtt tgccctttcc
    20941 gaaaataaag acattactct ggtcgtctgc ggtggcacgg tccgccataa aacgcgatct
    21001 atgcatggat ctattgctga acgttccctg caggatatta atgccgattt aatgtttgtc
    21061 ggtgctgatg gtattgatgc ggttaatggt attacgacct ttaatgaggg ttattccgtt
    21121 agcggtgcga tggtcacagc cgctaataaa gtgattgccg ttctcgattc atcgaaattt
    21181 aaccgtcgcg gttttaatca ggtactgcca atcgaaaaaa ttgacattat tattaccgat
    21241 gatgctgtat cagaggtgga taaactggca ttgcagaaga cacgggtaaa attaattacg
    21301 gtgtagtatt gttctgtatg ccggataagg tatttacacc gcatccggca tttcggattc
    21361 tccctggttc aagagagaat agtttgatta acgcaataat ggacaatctg gtggtaatcg
    21421 acaacgcaac gccgccggaa gtatttcaat atgaaagttt tgtccgctca acggttcgcc
    21481 atcaagatta aaagtgattt cgtgtggggc ttgtatatca aaccacgacg aagcgccttc
    21541 gataatactc gggttatctt cgtcagattt taaggttgat acgagagccg gaagaatttc
    21601 atcgccggta aaaatgcgca gttgcagcaa gccatcgtta attaacgcgt tcggacacaa
    21661 ttgctgaccg ccaccggcct gacgcccgtt accaatacca ataccagggc gtcaccttgc
    21721 cagtgaaagt tttcaccgcg gatttcacaa cggtccggtt gcagagtatc catgcgcatt
    21781 aagccatgaa tgatgtaaga gacgccaccc agcgcggctt ttaatttttc cggcgtttct
    21841 gtggtaatac gcgtcccaaa tccgcctgtc gccatattaa taaaacaggt ttgtttgttg
    21901 acctgcgcca tatctatcgc aatggcgtta ccggcaattg ccagtttcag cgccttatcc
    21961 agtgcctcag gaatccctac actggtggca aaatcattgg cggttcctaa tggcaaaatt
    22021 cccagcgcgg gtatgccatc cccctcacac tgaatcaacg ccgtagaaac ttcattaatg
    22081 gtgccatcgc caccaccggc aattaccgtt gcgacgccca acttccgggc ctcctctaca
    22141 aatcgtgcgg catcaccttt ctcccaggtg acccgcacat ggatcgtcat tccttcctca
    22201 cgcaacagca taattgcttc gcgcaagggt agattgtcag tacttttgcc attaagaatc
    22261 agtaagctgg cgggaaattc tgccatgatc gtatgtgcct ttatgattgg tctgtagata
    22321 gtgtagagca gaaaagaaaa ggtggagtca gaacaggatg aaagtcggag gataaaaagt
    22381 cagttcaagt atggaggatt cagtggttac caccaggagc ttgcaatggg aacagcgaat
    22441 attaacggta gccaccgata aatttcaaaa aagagcatat gcctaatatt caaccaaaca
    22501 gtggcatctt aaatatacta ttccaaatat attaaagccc catggagtta cctggaaagg
    22561 gccttcatgt ctgtaagtcc tacttatgta ggaaatgttg tacagaacat ttattataat
    22621 cctattcaaa tataatgatc agctattatt atatttaaac actagagagt gtcgttggta
    22681 tttaatgggg aaaggtgaga tgaaaaagat agctgctata tcattaatta gtatttttat
    22741 tatgtctggt tgtgctgtgc ataatgatga gacaagtatc ggtaaatttg gtcttgcata
    22801 taaaagtaat attcagcgta aactcgataa ccaatactac accgaagccg aagcttcttt
    22861 agccaggagt agaatatctg gtgcagaaaa tatagtaaaa aatgatgcaa ctcatttctg
    22921 tgttactcag ggcaaaaaaa tgcaaatagt tgagctgaag acagaaggtg taggattaca
    22981 tggtgtcgct cgtctgacat tcaaatgtgg agagtgagaa tattttttgg taagcatcaa
    23041 acatgcgcgt tctggttgtg cttagccgga acctgtgcga gcacgatgcc gttacgtgaa
    23101 aggcatcgtg ctatgaaggg agattctatc gatgtggtca atggaagacg gtgaccaggg
    23161 atagggctta tgcataaaaa ataagcccgt gtaagggaga ttacacaggc taaggaggtg
    23221 gttcctggta cagctagcat tttatgggtt atgtttttca gcgaaacgga tgataacctt
    23281 aataaatgca gctgtatgtg atcggtttct aagaattttc catccgggaa aaataatcga
    23341 aattaatcac ttaccgtgtg ggttacgcgt ggtttccccg gagaaattac gcatcagcag
    23401 cgcgtaattt agttcaagat cctgcgggac cgggagccac acagtataac catcgcctgg
    23461 tgcgaccggc atcgctacac ctttggcgtt ttccatgtgc tcaagggtaa agttaatgtt
    23521 gccttgcggc gtcatcagct caaggctgtc gccaacggag aatttatttt tcaccgctac
    23581 cgctgcgagg tcccccttgc gctcaccggt aaactcacca acaaactgct ggcggtcaga
    23641 aactgaataa ccgtattcgt agttctgata atcgtcgtga gtatgacgac gcaggaaacc
    23701 ttcggtatag ccacgatgcg ccagaccttc cagagtttcc agcaggctgg tatcgaacgg
    23761 tttgcccgca gcggcatcat cgatagcttt acgataaacc tgtgcggtgc gtgcgcaata
    23821 gtagaaagat ttagtacggc cttcgatttt cagcgaatgc acgcccattt tggtcaggcg
    23881 ttctacatgg gcgatggcgc gcagatcttt cgagttcatg atgtaagtgc cgtgctcatc
    23941 ttcaaacgcg gtcatatact cgcccggacg ctgggcttct tcgatcataa acactttgtc
    24001 ggttggcgcg ccgataccta gcgtcggctc aacattttgc accgggatcg gctcgtactt
    24061 gtgtacgatg ttgccaacgt catcttcttt cccttcctgg acgttgtact cccagcggca
    24121 ggcgttggtg caggtaccct ggttcggatc gcgcttgttg atatagccag agagcaggca
    24181 gcgaccggag taggccatgc acagcgcacc gtgaacgaag atctcgatct ccatatccgg
    24241 cacctgattg cggatctctt caatctcttc cagcgacagt tcgcgagaga ggatcacgcg
    24301 ggtcaggccc atttgctgcc agaatttcac cgtcgcccag tttacggcgt tagcctgcac
    24361 cgagaggtgg atcggcattt cagggaagta ctcacgcacc agcataatca gccctggatc
    24421 ggacataatc agcgcatccg gccccatttc caccaccggt ttcaggtcac ggataaaggt
    24481 tttcagcttg gcgttgtgcg gtgcaatgtt gaccacgaca taaaactttt tccccagcgc
    24541 gtgggcttca ttgatgccga gctgaagatt ttcgtggttg aattcgttgt tgcgcacacg
    24601 cagggagtaa cgcggctggc ccgcataaac agcatctgcg ccataagcga aagcgtaacg
    24661 catatttttc agcgttcccg ccggggaaag gagttccggt ttaaacataa ttctctcgtt
    24721 ctgatgacag gtcagatccg cttcacctga tgaagcggtt aggggagtgc ccccacttta
    24781 agggcgggca ttgtagcgca atcggcgggg ttggctccag tgcaaatgtg agggaaaaga
    24841 ctgggggatt tatttgttac cggccttcac tttatgtaaa aacagatcct tttcccacgg
    24901 ttgggcttcg ccactgcgta gtccctcggc aagtttttct cgtagcaagc gtaaagcttc
    24961 acgtattgtt tcgttttggt tccgctcgtc agcaggtaca tttgtcatcg taagtctata
    25021 attcgaagtt atttaaaagg aatgtaaccc cataatctgt tgtgaagttc cggattgcgg
    25081 gtaaaaagcg cctgaagttg ctgacgctga agagcattcg gttgaatgcc cgaaagattg
    25141 aaattgcgtt gccatatcgc gagctgttta cctgcaaatt cttcagttag cttgtgttgc
    25201 gattgaagaa gaagaattaa accgtcccat ttgttcaagt tatgatacag ccgacaatat
    25261 gcgcaagcat gtttaactga cggcgcgttt tcctgcaact gacgtatttc ctcgaacgtg
    25321 agcggaacgc gacaccatac acagatgtta accataaatc gcaacccggc ggcgtccggg
    25381 ttagccagcg tggtcagaat agtctctttg acgccacaac gaggattagc ttttgcaaga
    25441 atgtgcatag cggctaaacg cagtgggaat acattctgtg ataatcccca ttctgccagt
    25501 tttaaaatat tgggatcatg aatggaatcc agcccgtata aagcggcccg tcgctgtgag
    25561 ggagtatatt ccgcagaagt gacaacatga cgccagaatt gcggaacatc catgacttcg
    25621 tgaagcatat ttttagcgcg taggcggatc aacatattgt tatccagcag taatgtgctg
    25681 tgcaacgccg gaggcatttc tattgctcgg tcagtgaggc taaataacgt tgcttgtcga
    25741 atacgcggcc agcggtcttt taacagacga gttaataccg cttcgcttaa tggtaattgt
    25801 tgacgcagcc agtgggtggt cgccagcatt cgcaaaccct catcgggttt atcgagtatt
    25861 gaaagcagtg aagattcatc attaaagtaa ccgtattcaa tcagcgtttg taataccgcg
    25921 cgggagagct ttttatcagg gcaagtaagc caatacgtta attgtgggcg gttattttct
    25981 gtcaataaat atctaagtat ctcgtctaca aatgcacggt gatcgtaacg ctggcattgg
    26041 agaaggttac gtatcatggg tagattaacg gcaaatgccg ctgcattctt atccttcatt
    26101 aaatgacgta atgctatggt tgcagcaagg cgaacctctt ttacccagtc attagctgca
    26161 agtaataatt cttctatagc cgcaacatcc tctttgtgcc ccagatactg cacggccttt
    26221 tctcgtatat atccattgtg atgatgggta agctggcaaa gaaccagagt atcgttgaga
    26281 tcacaatcat gtgctaaata attgcctggt tcggctatca atgcctggag ttgttgctgg
    26341 atgagaagta attcacagtc aacagaatta ttcatttcga cgttaaacaa aaccctgcgt
    26401 gaaaataatg tacgcaatcc tttgagcata attttcctta tcagttatga ggagaaaact
    26461 catttcatgg ttaataattt cttgagggat agtaactctg tgatgagaga aaataaagcc
    26521 ggacgccaac aacccacgcc cggcatcgta attacgtatt gtctttcacc gtctggcttg
    26581 tgccattggc tttaacggag gcaattccgg tttcacgaga ttgcgcggtg gcgtacatct
    26641 ggctggagcc gataatttga tgattggcgg ctttcagatt gaaatagaat ttgccgttac
    26701 ttgccgtttt tttctcatag cgttcttctt gcgggctgtt gctacgcact gacgcgatgc
    26761 ccttttccgc agaggctttt gatgtataca gctcgctggt gaggatagtc tcaccattgc
    26821 ccgcttttag cacaaaccgg aactgattat cactgctctt actgagttca aaccaaccag
    26881 ccataatgac tccttgattg aaattgcgta tcgaaaacag catctaaagg tgctgtttac
    26941 cgattaaagt atgagcgatt ttctgatgcc tgccagggcg gagagataat gtcagtttgt
    27001 tgaccatgcg tgggataatg ttgccggatc tggtctgaac gccatctccg gctaacaaag
    27061 tcatgtcgct aaaactaaac gatgcggcag gcgtcggctt cccagcggta accaacgccg
    27121 taaacggcgc ggataaatga ctgttcggcg tcgagagatt ccagcttgcg gcgcaggttt
    27181 ttaatgtggc tgtcaatggt gcggtcggtt actacgcggt agtcgtcata aagatgattg
    27241 agcaattgct cgcgggagaa cacttttcct ggttcatgag agagcgtttt caacagacga
    27301 aattccgcag gtgtcaggtc aagcatttta ccgcgccatg aagcctgaaa acgaccttcg
    27361 tcgataatca acgggctttc ggcatcctgt tgctgcaatt cgcgctgcgg tttgcaacgg
    27421 cgcaaaatgg ttttgacgcg cgctaccact tcccgtgggc tgtacggctt gcagatgtag
    27481 tcatctgcgc caatctccag tccgagcagg cggtcgatct cttcgatttt tgccgtcacc
    27541 atcacgatag gaatgtcaga aaaacgacga atttcccggc acagcgtcaa gccatcggtg
    27601 ccagggagca tcagatccaa caggatcaga tccggtggtg tctggcgcac atacggcagt
    27661 acctgatcgc cgtggctgat aagcgtcggc gcatagctcg cagcacgcag ataatcaatg
    27721 agcaactgcc ccagcttcgg ttcatcttcc acgatcaaaa tacgtggtgt gttttcgtcg
    27781 attggtaact cggtcatact tctctctgta aatcccgttc cagcggtaac tctactgtaa
    27841 tgcttacccc gccaaaaggc gaatgggcag caataatgcg gccattatgt gcttcaacaa
    27901 tgttcaggca aatcgccagc cccagcccgg aaccaccgct ggcgcggttg cgggaacctt
    27961 cggtgcgata aaaacgttca aataattttt gtagctgctc gtcactgaca cctggcgcac
    28021 tgtcggcaaa ggtcaggcgc accgttttgt cgtgctgctc ggcagagatt ttcaggctgc
    28081 cgccgctgtc agtatagcgc aggctgtttt caagtaaatt attgaataac tgcattaaac
    28141 ggtcgcgatc gccaaatacg gtaatactgt ctggcaggga aaattgcagt ttcaggccac
    28201 gactggcgaa tcgttcgcga aatgcgccgc ccgccacttc cagcagtggg atcaaatcta
    28261 ccggtgcttt ttgataggcg agagcgcctt catcagacat cgacaactga tggagatcgt
    28321 caaccagttt ggtcagtgta ccgacctccg cctgtaaaga cgccaccgtc tccggcgtga
    28381 atttacgcac gccatcctga atggcttcca gttcaccgcg cagcaccgct aatggcgtac
    28441 gcagttcgtg agaaatatcg gccataaaat cgcgccgcat ttgctggttt ttctccagtg
    28501 tgctggcaag ctggttgaag tcttgcgcca gtttgcccag ttcatcttca ctggtgggcg
    28561 ttacgcgggt agtgaaatcg cccgccgcca gtttgtgcgt gccatcgaca agtcgtttta
    28621 ccggtgccag taaaccgcgc gccagcagaa aagtggcaag tgccgcgagt aacgttgcca
    28681 gggcgacaat caaccagctg gtttgccgct gttgtttatc gaaattgata tcagtattgc
    28741 gcgttaaccg ctcaacgggg gaggcgatca ccgcgccaac ttccgcaccg ttgaccagaa
    28801 tgggtcgccg tgtaccgtca ggtggaatcg gcgctcgcgg accaaccagc actttgttgt
    28861 tttgatcaac cacccagaac tgggtacgcc agccgtgcgg tggcataccc gggccgggtt
    28921 tatcttccga attatcgtgt tcaaatgaac gcaggatctg aaagacaaag cgatcattgt
    28981 tgcgcaggaa gcgccagttg ccatgctgcg catactgctc gccaagcgca tcacttaaca
    29041 gttgtaatcg ctgttcatta ccatgcttga tgtaatcaat aaagccacgc tcaaaactga
    29101 tacgcaccgc ccagtgcata ctgatcagca agacaatgca ggtggcgaaa atcgccagaa
    29161 acagtttgcc ggtaataccg ggtcgccaga acttcattgc gcgctccttt ttcgccgcga
    29221 aatagctaca ttttgatgcg tatcgttcgg cactctggca aagatgaacg ccggaagggc
    29281 gatgatcaac gccatgctaa gccaggtgta cataaagacg gtttgtgtgg tgccgctgtc
    29341 gacgctgaca tgctgtgaac caaaaagtcc cagcaacagc ccggcgatag tgacgccgat
    29401 actcatcgac aattgcataa tcatcgacag caggctgttg ccgctgctcg ccagattgtc
    29461 cgggagatct ttcagcgtca gggtgttcat ggaggagaaa cgcgtcgagt tgaccatccc
    29521 ttgtaaaaac aggacgaacg gcaaaacgta gtaccagccc agcagggcgg tagtcataaa
    29581 caacagggtg accagcgaca gacccagcgt ggtcgctacc agtacccgac gataaccaaa
    29641 gcgattcacc acctgtacca caattcgctt cattcccatg ctgccaagca ccatcgggat
    29701 catcatcagt ccggcatgaa acggcgagaa accgaggcca atttgcagga aaaccggtgt
    29761 cataaagggc aacatgccac tgccaatacg tccggcaaag ctccccgcca ggcccagcga
    29821 aaaggtacga gtacggaaca gtttcagact gaacagggca cggttgttat ttctggcgtg
    29881 cagcagataa agcaccagtg ccaccacgcc aactgcgacc aggccagcaa tcgccagcgg
    29941 cgataaacct gtacctttac tgccgtccag cgccaggggt taataccgcc atgccaaccg
    30001 ccagcaataa aaatccggag agatcaaagc gccacgtctg catggtgtag ttcggcatta
    30061 acagcaatgt ggcgatcgca ccgataatcc ccaccggaat gttgatcaaa aagatccagt
    30121 gccacgacgc gtactccacc agcagaccgc cgagcgccgg accgagcagc ggaccgacct
    30181 gaccgggtaa cgtgacaaag gtcatcgcag ccatatattg ctcgcgcggt acgattttca
    30241 tcaccgtcaa tctgccgacc ggcaccatca tcgcgccgcc aacgccctgt aacgcgcgtg
    30301 ccagcaacag ttcgttcagc gtgccggaaa gcgcgcaaaa cagtgaaccg agagtaaaca
    30361 gcacgatggc ggtaaagaaa atattgcgca cgccgacttt gtccgccagc cagccgctgg
    30421 cgggcagcat caccgccacg gtcagcacat aagagacaat gaccatgtgc atatgcaacg
    30481 gactttcccc gaggctttgc gccattgagg gaagggcggt gtttacgatg gtggtgtcca
    30541 gcgactgcat aaagaagccg aaagccacaa tccacaattg ccaacgggtg ctgtcgggaa
    30601 gatctgtcat ttactcggtt accgtttgtt taggtttacg cgaaaaacgc agccgcagac
    30661 ggtcgaaaaa gagatacacc accggcgtgg tatacagcgt aaggagctgg ctcattacca
    30721 gtccgccgac aatggtgatc cccaggggtt gccgcagctc cgagccgtcg ccgcccgaca
    30781 ataccagcgg cagcgcacca aacagcgccg ccagggtggt catcataatc gggcgaaaac
    30841 gcagcagaca ggcctggaaa atagcttcct gcggcgtcag gttaccgtgc cgttgggctt
    30901 caagcgcaaa atcgaccatc ataatggcgt ttttcttcac gatgccgatt aatagcatga
    30961 tccctatcag ggcgattagg ctgaacgggg cattgaacag ctccagcgcc aacagcgctc
    31021 caacgcccgc cgagggcagg gtggagagaa tcgtcagcgg atgtacgtaa ctctcgtaaa
    31081 ggatacccag cacgatatac accgtggcga tggcggcgat aatcaggatc acctgcgagt
    31141 tcatcgtctc ctggaacacc tgcgccgtgc cggcaaaact gccgcgcacc gtcgaaggca
    31201 ccccaagctg ggtcattgcg cgatcgatcg ccgcactggc gtccgagagc gattttccgg
    31261 tcggcaggtt aaacgaaatg gtcgaggccg ccgataatcc ctgatgattc accgatagtg
    31321 gggcattcgc cggttgccat ttagcgaaat acgacagcgg gatcgctttg ccttcgttat
    31381 tgataacgaa cattttttcc agcgcactga tgtcctgggt atagcgcggc tccacttcca
    31441 tcaccacttt atactggttc atcggctggt aaatggtcga gatttgccgc tgaccgaagg
    31501 cgttatttaa cagactgttg gcggcttgta cgtcgattcc cagccgtgcc atggtgtcgc
    31561 ggtcgtaaac cagattcatc tccgcgccgt tatcctgctg atcggagttc acgtccgcca
    31621 gttccggcaa cgtcgccagt tttttgcgga ttttcggctc ccattctcgc agtgccgcca
    31681 ggtcgtcgga taacaacgtg tactggtagc tggcgttcga ctgacgccca ccaacgcgaa
    31741 tatcctgtac cgccatcagg aacagattcg cccccggttc tttcgccagt tttacgcgca
    31801 gacggtcgat aatttgctgc gccgtttcgc tgcgttcgtc gcgtggcttg agggtgataa
    31861 acatcatccc gctgttcact cgcgaaccgc ctgtaaagcc ggtgacatta tccactgccg
    31921 gatcgtcacg gataattttc atgaaatcct gcaacttacc gcgcatcgcc tgaaacgaaa
    31981 tactctgatc cgcctgaatc ccgcccatca acacgccagt gtcctgctcc gggaagaagg
    32041 ttttcgggat cgagatatac agccagatat tcagcgcaat ggtgccaagc agcaccacgc
    32101 ccaccagacg ggtatgattg agcacccatt tcagtgactt gccgtagcct tgttgcaggg
    32161 ctaccaacat gcgaccaaaa ccacgcagtc gcttttgctc gcgcggcttg ctggctttca
    32221 gcatccagcc acacatcatt ggcgttaatg tcagagaaac cagcaacgat ataccaatgg
    32281 cgacagaaag cgtcacggca aattcgcgta acagtcggcc cggcaatccg cccatcaaca
    32341 gcagcggcag gaacaccgcc accagtgaca gactcatcga cagcaccgta aaaccgactt
    32401 cgcgagtacc ttgcagtgcg gcttgcaacg gtttcattcc cgcttccaga tgacgggcaa
    32461 tgttttccag caccacgatg gcgtcatcca ccacgaaacc agtagcgatg gtgagcgcca
    32521 ttaacgaaag attattgaga ctgaaaccgc acaggtacat cgccgcaaac gtaccaatca
    32581 gcgaaaccgg caccgcaacg gcgggaataa tagttgcgcg acccgagcgc aggaataaaa
    32641 acaccaccag aatcaccagc gccaccgaga taatcagcgt ttgctcgact tcttccagcg
    32701 aggcgcgaat ggtgggggag cgatcctgag caatttgcag atcaatcgcc gccggaatgg
    32761 tttcctgcaa ctccggtaat tttgcccgga tgctgtcaac cgtctggata atattggctt
    32821 ccggcagctt gcggatcatc agcaaaatag ccggtttggc attggtcatc ccggcgttac
    32881 gtacatcctg tacagagtcg gtcactgtcg ccacatcgcc cagacgaacc gcgccgccgt
    32941 tgttgtaatg aataatcagc ggctgatatt cggcggcggt ttttagctca tcattggtct
    33001 ggatctgcca gcggtgagtg ccatcttcca gcgccccctg cggtttacgc acgttggcat
    33061 tgctgatggc agtgcgtacg tcgtccagcg agacgccctg attaaacagc gcctgcggat
    33121 tcagcccgac gcgtacggcg ggcagtgagc tgccgccgac atcgacatca ccaacaccgt
    33181 cgatttgcga aatcgtcgga gccagctgcg tcgaggcgaa atcgtacagt ttaccctgcg
    33241 aataagtatc ggatgtcagc gtgaggatca taattggcgc atccgacggg ttagctttgc
    33301 gataggtcgg acggctgggc atcccgctgg gcagcaaact ttgtgcagcg ttgatcgccg
    33361 cctgcacatc gcgcgccgcg ccgttgatat cccggtcaaa atcaaactgc aaaataatac
    33421 gcgtgctgcc gagcgaactg ctggaggtca tttcactgac tccggcaatg cgcccaagtg
    33481 agcgctccag cggcgtggca acggaagacg ccattgtttc tggtgacgca ccgggcagcg
    33541 aggcgctgac cataatcacc ggaaaatcga cctgcggcag cggggcgacc ggcagcatac
    33601 ggaagcccag tatgccgcac agggtaatgg caaccgacag taaaatcgtc gccaccggac
    33661 ggtaaatgaa gagggcaaaa aacttcactt acgcttcctc ttcatggcga gcaaagcgac
    33721 ttttggtcca caatgccagg cggtcgaaca gcagataaat caccggcgtg gtgaacagcg
    33781 tcagcacctg gctgacaatc agaccgccga ccatgccgat acctaacgga cgacgcagtt
    33841 ccgcgccgac cccggtactc aacatcagcg gcagcgcgcc aagcagagcc gccagggtgg
    33901 tcatcaggat cggacgaaaa cgcaacagac aagcctggta gattgcatcg cgcggcgaca
    33961 tgccttgctc gcgctcagca gccagcgcga agtcgatcat catgatggcg ttcttcttca
    34021 caataccgat cagcaaaata atgccgataa tcgcaatcac atccagttcg ctaccggcaa
    34081 tcattaatgc cagcagcgcg ccgacccctg cggtgggtag cgtcgagaga atggtgatcg
    34141 ggtgaataaa gctctcgtac agaatgccga gcacgatata catcgccacc accgccgcga
    34201 caatcagcca gacagtgctg cccagcgccg actggaaggc gagggtgctg ccctggaatt
    34261 gcgtggtgat atccaccggc agattcagcg tcttttcggt gtccataatc gcctgcaccg
    34321 catcgcccag cgaatagtta tccggcacgt taaaggagat ggtcgttacc gggaactgat
    34381 ccagatggtt gatggaaagc ggcgcaaaac gctgttcaac tttggcaatt gagcttaacg
    34441 gcaccacgcc gccgtcgcta ctggtcaggc gaatggtatc cagcgccgcg aggcccgggg
    34501 taatttcggt gttgtgctcc agcactacgc gatactggtt agcctgagta taaatagtgg
    34561 aaatcagtcg ctgaccaaac gcactgtaca gggcattatc gacatccgcc atgctgatcc
    34621 ccagacggct ggcgctgtcg cgatcaacat tgacatacgc caccagccct ttgtcctgcc
    34681 agtcgctgga gacatcagaa agctgtggca gttgctggag tttttccatc aactgtggca
    34741 cccaggtact gagcgcatcc agtgaggtgg cctgcaaggt aaactggtac tgggtgcggc
    34801 tgacctgagt atcaatagtc agatcctgcg ttggttgcag gaagagatcg acgcccggca
    34861 ctttatctac cgccgtttgc agacgggcga tgactttttg cacccgatca tcacgttcat
    34921 ccaacggttt gaggttgatt tgtaaacgtg cactattcag cgacgggtta gtgccatcaa
    34981 cgccaacaaa tgaggtcagg ctttgcactg ccggatcctg caaaatcacg tccgcgacct
    35041 ggcgttgtcg ctgggccata ttggcaaagg agctggattg cggtgcctgc aaagtgccct
    35101 gaataatgcc attgtcctgt accgggaaga aacctttcgg aatgaacacc cacagcagca
    35161 cgctaagcag cagcgtgctg agtgccacgc ttaaggtcag ccacggatga ttcagcactt
    35221 tcgccagtcc acgaccatag gcggcgatta tcctgtcgaa cattttttcc gaggcacggg
    35281 agaagcggtt ctgtttacgc aacgactcct ggctgagcat ccgcgcgcac atcatcggtg
    35341 tcagggtcag cgacaccacc gctgagatca aaatcgctac cgccagggta atagcaaatt
    35401 cgcggaacag tcgcccgacg atatcgccca taaacagcag tgggatcaac accgcaatca
    35461 gtgagaagat cagcgagata atggtaaagc cgatttcacc tgcgcccttg agcgccgccg
    35521 ccaacggttt ttcgcctttt tcgatatagc gggaaatgtt ttcgatcacc acgatggcgt
    35581 catcgaccac gaatccggtg gcgatagtta acgccatcag tgtcaggtta ttgattgaaa
    35641 aatcgagaaa caccataacc gcgaaagtgc cgattaacga cagcggtaca gcaacaccgg
    35701 gaatgatggt cgccggaata ttgcgcaaaa acaggtagat aatcatgact accagcgcga
    35761 tagccatcat caattcaaac tgagtatcat cgacggatgc gcggatattg gtggtgcgat
    35821 cggaaagcac tgtcaccttc accgatttcg gcagactctc agtgagctgt ggcagcatct
    35881 gccgaatgct gtcggcggtg gagataatgt tagcaccggg ctggcgctga acattcatca
    35941 caatggcctg ttctttgttc gcccacgcgc cgagccagct gttttctgca ccttgctcta
    36001 cagttgcgac atcgcccaga cgaattggcg cgccgttctg gtaggcgatg attagctggc
    36061 gatactcttc ggcggattgc atctggtcgt tcgcggaaag cgtgaccgca cgggaagggc
    36121 cgtcgaggct accttttgcc gagttaacgt tagcgccggt aatggcggtg cgcacggttt
    36181 cgctggtcag gccgagggcg gcaatcgcct gagcgttaag tttgacgcga acagccggac
    36241 gctgaccgcc ggaaagcgtc accaggccga cgccggaaat ctgcgagatt ttctgcgcga
    36301 cgcgggtttc caccatatct tccacttgcg tcatcggcat ggcggttgag gtgacggcga
    36361 gcgtcatgat cggcggatct gccgggttca ctttgctgta aaccggcggg ttaggcagat
    36421 cgctcggcaa caagttggtc gcagcgttaa tcgcggcctg cacttcctgc tcggcgacat
    36481 cgagcggtaa tgttagctgg aactgcaaag tgataactga cgcaccgccg gaactttgcg
    36541 acgacatctg tttcaggcca gacatctgcc cgaactggcg ttcgagcggc gcggtaacgg
    36601 cagaggtcat gacatccggg ctggcacctg ggtagagcgt gaccacctga atggtcggat
    36661 agtccacttc cggcagcgcc gaaacgggca gggcgcgata accgataatc ccggcgagta
    36721 agatcgccac catcagcagc gtggtggcga caggacgcat aataaacagg cgcgacgggc
    36781 cgcctgtgct gctcgggggt aacacctgca tcaggagcgt gctccttttt tcgcgtattc
    36841 gcggctggtg gctttctctt ccggagtggt ggcgctctgg gcttccacca cttccacttt
    36901 cgccccttcg gtcaggcgat caatgccgtc tgtcaccacg cgatcgcccg cagaaatacc
    36961 tgcacggatc accactttct gactgtcctg aatgcccggc gtcaccagat gtttgctgac
    37021 cttgttttcg ctattcagca cccagacaaa atggccttca ttgcccattt gcagggcggc
    37081 tgttgggatc actacggcgt tttgttcggt gtcgactaac atgcgcgcgt taacaaactg
    37141 attgggaaac agcacatcat tctgattatt aaagcgtgct ttcactttaa tcataccggt
    37201 tgtggcatcg atttggttat cgaggcttaa cagcgtgcct tcacttaatt tcttcgaatt
    37261 ggtgcgatcc caggcttcta ccaccagcgg ttttccggct ttttgcgcct gcactacagt
    37321 agcgatatcg ctttccggca gggtaaagac taaatcgata ggatgcgtct gggtgatcac
    37381 cacaattccg gtggtatcac cactggagat ttggttacca acatcaacct gcttgagacc
    37441 aacgcgacca tcgactggtg cggtgatgcg gctccagtcg agttgcaact gcgcgctggc
    37501 gacgcttgct tcatcagcct taatggtgcc ttcggtttca ctgaccagcg cctgttgggc
    37561 atccagctcc tgacgggaga cgagattggt ttttgccagt tgctgataac gcgccagatc
    37621 acggcgggcg ttagcaagcg tggctttatc ttttgccagt tggccctggg tttgtgctaa
    37681 tgcaactttg aactggctgg ggtcaatttc tgccagtaaa tcgcctgctt tgacctgctg
    37741 gccttcctgg aaatgtaacg ccatcagttg accgtccacg cggctgcgca ccgtaacagt
    37801 attagcggcg ataatggtgc caagcccggt gaggtaacgc ggaactgcct gttctacggc
    37861 ggtcgccgcc tgaaccgggg ctaatgggcc ggaacgcata ccacggcgac cacccgctgg
    37921 cgattgctgc gcttgtttcg tcgcccctgg ggctgcactc cgggagtcat tgcggccttg
    37981 ccagaaccag aatgcggcga tggcggcgat aaccaccacg attacgatta cccaacggga
    38041 tttataactg cctttcatcg ttaagagttt ctcttcctga aacgtttcgc ggaatgatac
    38101 tagtgtagcc agccaataag ggagaaaaat ggaggaatta tgaaacactg tcgggaattg
    38161 tctgaatgac gggcacattt gcgagcacgc atccagtaat aacacaggaa actattttat
    38221 ctacgcgtta gggatagact gcttgcatgg cgaaaggagg taagccgccg atttcagcgg
    38281 gacgctgaaa cgggaaagcc cctcccgagg aaggggccat aaataaggaa agggtcatga
    38341 tgaagctact catcatcgtg gtgctcttag tcattagctg gccagcttac taagactacc
    38401 agggcggggg aaaccccgct ctaccctcac tcctgaaagt atgccttcac gataagattg
    38461 tcaatctgca ggctttgcag tctgcgatcc tgccagcaaa tattctttgc gagacgttac
    38521 gcaataatca cactggaaac tattttatcc gcaagtcagc gatagactgc atacagggcg
    38581 aaaggaggta agccgacgat ttcagcggga cgctgaaacg ggaaagcccc tcccgaggaa
    38641 ggggccttaa ataaggaaag ggtcatgatg aagctactca tcatcgtggt gctcttagtc
    38701 ataagcttcc ccgcttacta agactaccag ggcgggggaa accccgctct accctcactc
    38761 ctgaaagtat gccttcacga taagattgtc aatccgtagg ctttgtagtc tgcgatcctg
    38821 ccagcaaata ttctttgcga gtcgttacgc aataatcaca gaggaaacta ttttattcac
    38881 gcgttagcga tagactgcat tcagggcgaa aggaggtaag ccgatgattt cagcgggacg
    38941 ctgaaacggg aaagcctctc ccggagaaga gggcttttaa taaggaaagg gttatgatga
    39001 agcacgtcat catactggtg atactcttag tgattagctt ccaggcttac taagaacacc
    39061 agggggaggg ggaaacctct ccctaaccct cactcctgaa attatgtcac tccgccctta
    39121 gtgtcaatcg acggctctcc gttcttgcga ggcgaatagc aaaattttct gcgccgacgg
    39181 gcctaattct gaaagtttct tcgtttgctc ttatttcatt attgcaatcg gtatgatacc
    39241 ggacctcgat aattctctaa gaaaggattt cttatgagcg aacaaatcac gtttgccacc
    39301 agcgattttg ccagtaaccc ggaaccacgt tgcccctgca ttttgctgct ggatgtttct
    39361 ggctcgatga gcggcagacc tattaatgaa cttaacgccg gattggttac ctttcgtgat
    39421 gaactgcttg ctgatccgct ggctttaaaa agagtggaac tcggcattgt gacgttcggc
    39481 ccggtgcatg tggaacaacc ctttaccagc gccgctaact ttttcccgcc catcctgttt
    39541 gcccaggggg atacgcctat gggggccgcc attaccaaag ccctagatat ggttgaagag
    39601 aggaaacgtg agtaccgcgc aaatggtatt tcgtattatc gcccgtggat tttcctgatc
    39661 accgatggcg ctccaaccga tgagtggcag gctgccgcca acaaagtgtt tcagggggaa
    39721 gaagataaga agtttgcctt cttttccatc ggtgttcagg gggcggatat gaagactctg
    39781 gcacaaatca gcgttcgtca gcctttgcca ctgcagggat tacaattccg tgaactgttt
    39841 agctggttat ccagttcgct gcgttcggtt tcccgctcca cgccgggaac ggaagttgtg
    39901 ctggaagcgc caaaaggctg gacgtcagtg tgagctggcg tctggtttat gcatccgctg
    39961 ttggaacatc gcacattagt gctgatttgc cttgtcagga tgcctgccag atgcaggttg
    40021 tctggttaaa cgaccagcaa ccattactgg tgatgttcct tgctgatggc gcaggtagcg
    40081 tttcgcaggg aggagaaggc gcgacgctcg ccgtcaatga agcgatggcg tttatggtgc
    40141 aaaaagtaca ggacggtgaa ttggggctta atgatgtcct cgccacaaat atggtactga
    40201 ctattcgtca acgacttttt gccgaggcag aagccaaagc attagcagtt cgcgattttg
    40261 cctgcacgtt tctgggattg atttcatcgc ctgacggcac cttgattatg cagattggcg
    40321 atggcggcgt ggtggtagat ttgggtcacg gtctgcaatt gcctctcacg ccgatggtgg
    40381 gtgaatatgc caacatgacg cacttcatta ccgacgaaga tgccgtttcc agactggaaa
    40441 ctttcaccag cactgggcgt gcgcataaag ttgcagcatt tacggatggt attcagcggc
    40501 tggcgttaaa tatgctggat aattctcctc atgtgccttt ctttaccccg tttttcaatg
    40561 gactggcgtc agtaacgcag gaacaactcg atttattacc tgaattgtta aagcagtttt
    40621 tgtccagccc ggcggtaaac gagcgtacgg atgatgataa aacgttggca ctggcgctgt
    40681 ggttaccatg aaacccactt tatatactgc tactggtgag tgcgttacgc caggccgtga
    40741 actgggcaaa ggcggtgaag gcgcggttta tgatatcgag gagtttgtcg atagcgtcgc
    40801 caagatttat cacacgccgc cacccgcctc aaaacaggac aaacttgcct ttatggctgc
    40861 gacagctgac gcgcagttgt tgaattatgt cgcctggccg caggcaacgc ttcacggtgg
    40921 acgaggcgga aaagttatcg gttttatgat gccaaaagtt tctggtaaag aaccgattca
    40981 tatgatctat agcccggcac atcgtcgcca gagttaccct cattgtgcgt gggattttct
    41041 actctatgtt gcgcgcaata ttgcttcatc ttttgctacg gttcacgagc acgggcacgt
    41101 cgtgggtgac gtaaaccaga acagttttat ggtaggtcgc gacagcaaag tggtactgat
    41161 tgatagcgac tcctttcaga ttaacgtcaa tggcacactg catttatgcg aagtcggcgt
    41221 gtcgcatttt acgccgccag agctacaaac cttgtcgtca tttgtcggct ttgaacgcac
    41281 agaaaatcac gataattttg gccttgcgtt gctgattttt cacgtcttgt ttggtggccg
    41341 gcatccttat tctggtgtgc cgcttatctc tgatgcgggt aatgcgctgg agacggatat
    41401 tacccatttc cgttatgcct acgcgtcaga taatcagcga cgtggtttaa aaccgccgcc
    41461 acgatcgatt ccgctatcga tgttaccgag tgatgttgaa gccatgtttc agcaggcatt
    41521 cacggaaagt ggcgtggcaa ccgggcgtcc gacggctaaa gcgtgggtag cggcacttga
    41581 ttctctgcgc caacagttaa agaaatgtcc cgtttcggca atgcatgttt atcccgctca
    41641 tttgaccgac tgcccgtggt gtacgctgga taatcaaggc gttatctatt ttgttgatct
    41701 cggtgaagag gtcattacca ccagcggtga ttttgtgctg gcgaaagtct gggcgatggt
    41761 gatggcgtca gtagcaccgc cagcattgca attgccatta cccgatcatt tccaaccgac
    41821 tggcaggccg cttcctttag gcctgttacg gcgcgaatac atcattctga ttgagatcgc
    41881 actgtcagcg ttatcgctgt tgctttgcgg ccttcaggca gaaccgcgtt atattatttt
    41941 ggttcctgtg ctgtcggcta tctggattat tggcagtctg acaagcaaag cgtacaaagc
    42001 agaaatccag caacgccgtg aggcatttaa tcgcgcgaaa atggactatg agcatttagt
    42061 cagccagatc caacagttgg gcgggctgga aggttttatc gccaaacggg cgatgctcga
    42121 aaaaatgaag gacgaaattc tcgggttacc ggaagaggag aaacgcgctc tggcagcact
    42181 ttatgacacc gcaagggaac ggcagaagca gaagtttctg gagggatttt ttgcctctat
    42241 tcccggcgtt ggccctgcgc gtaaagcggc gttacggtct tttggtattg aaacagcagc
    42301 ggatgttacc cgtcgtggcg ttaagcaagt taaagggttt ggcgaccatc tgacccaggc
    42361 ggttatcgac tggaaagcga gctgtgaccg ccgttttgtg ttcaggccga acgaagcggt
    42421 aacgccagca gacagacagg cggtaatggc cgccaaacga catcggctgg aatcggcgtt
    42481 gactgtcggc gcgacagagt tgcagcgatt ccgcctttat gctccagcac ggaccatgcc
    42541 gttgatggaa ccgttacgtc aggcggcaga aaaactggct caggcgcagg cagatttaag
    42601 ccgctgctga gccattttct tttaacgaaa taccacttcc gcccagcgtg ccagcccggc
    42661 ggtgacggag ccaaagtcat cgccgcctgc aatcgaaatg cccggcaact gttctgccag
    42721 agccttttta atcagcggcg aacgggcgct accgccggtc aggtatataa cgtccggttt
    42781 ttcctgggcg ttatccagcg ccagttgcac ctgttccaga atccgcgcca gtggctggct
    42841 gagggcgctt tccagccctt gctggctaat cagcgtagcc agttcatcgc tgataaacgg
    42901 cagtgaggcg cgggtttccg cagcgtttga aagggcgatt ttgctctctt ctgcgctgcg
    42961 taccaggcga tagcttaaac gctgacgcca gactttctgt aacagggcca ctttttccgg
    43021 ttcgcgggca tcgcgtacca gatcgttaag cagacgaccg ttggcactac tgtagaaatc
    43081 actctgcgca ggtacgtcgt tgatggcaac cgcattccac cacggcagga tcggcagggc
    43141 gatgcctttt tcggtttcgc cacccatacc cagcagtggc atcaggtttt taaacgccag
    43201 ggcgatatcc agatcgttac cgccaatacg acaaccactg tgacccagca ggctggcttc
    43261 acgatcgaga cgcgaacgcc actgcggtcc catcagcagc aatgaacagt cagtcgtacc
    43321 accaccgata tccaccacca gcacccgttt ttcttcctgc aaggtggctt cgtaatccag
    43381 cccagccgcg accggctcat actggaatac cacgtccctg aatccggcac gcttcgccgc
    43441 gcgttccaga atcccttgcg cctgggcgtt tgcttcatcg ccgcccagcc cctggaagtt
    43501 gatcgggcga ccaatcaccg cctgagtaat cgcttctggc agctgcgcct gcgcctgctg
    43561 gcgaatgtgc aacatcattg cgcagaccag atcctcaaac agcgctacct gctgcggttt
    43621 taagccgctg gcaccgagga acgattttgg tgatttcaca aaccacactt cttctggatc
    43681 atcaatgtac tgtgccagtg aggaaagacc gaactgcacg cttttcgctg taacatcgat
    43741 atcttcttcg cggttataac gaatcgcccg tcgtaacaac gcctgcgttt catcgtcgtc
    43801 tgccggaacg tcatgatggc ggtacagcca ttcgcttacc gcttcacgcg ttggcgcgca
    43861 aagcattgaa ggcagcagcg tgctgtcgtt ttccattttt agcaagtgcg gtttaccgtc
    43921 acgcatgacc gccactgaac agtttgctgt accgtaatca aaaccaataa acacgaaata
    43981 atccccatgc cggtgaagaa ggggcgtgac tttagcgaaa tgttgccgtc gcgacaaccg
    44041 gaatatgaaa gcaaagcgta gcgtctgaat aacgtttatg ctgaaagcgg atgaataagg
    44101 agatgcgatg tataccctga actggcagcc gccgtatgac tggtcgtgga tgttgggatt
    44161 tctcgccgcc cgtgcagtga gtggtgtgga aacggtcgct gacgattatt atgcccgtag
    44221 tctggcggtg ggcgaatatc gcggcgtggt gactgctatt ccggatatag cccgccatac
    44281 tctgcacata aatttaagtg caggtttaga acccgttgcc gctgagtgtc tggcgaaaat
    44341 gagccgcctg tttgatctgc aatgtaaccc gcagattgtt aacggtgcgt tgggcaagtt
    44401 aggcgcggcg cggcccggat tgcgtttacc cggctgtatt gatgcttttg agcagggtgt
    44461 gcgggcgatt ttaggccaac tggtgagcgt ggcgatggcg gcaaaattga ctgccaaagt
    44521 tgtacagctt tatggcgaac ggctggatga ttttccggag tatatctgct tccccacgcc
    44581 tcagcggctg gcagcagccg acccgcaggc attaaaagcg ttaggcatgc cgttgaaacg
    44641 ggcagaggca ctgattcatc tggcaaatgc ggcgctggag ggcagcttac caatgacaat
    44701 tccgggcgat gtggagcagg cgatgaaaac gctgcaaact tttccgggta tcgggcgctg
    44761 gacggcgaat tatttcgctt tgcgtggctg gcaggcgaaa gatgtttttc tgccggatga
    44821 ttatctgatt aaacagcgat ttccggggat gacaccggca caaatccgcc gttatgccga
    44881 gcgctggaag ccttggcgtt cttatgcgct gttgcatatc tggtatacgg aaggctggca
    44941 accagacgga acggacgaat tgtaggcctg atacgtcgca tcaggcaatg ggcaaacgct
    45001 ctctcagtta atcgcgaaat aactgctatt caccagcaaa tccagcggtt gggcatcggc
    45061 aatcacatca ccataaatca gatcgacgcc gatgccagaa agcgtatcca tcactaaggg
    45121 taaaacgact ggcccggcga tggttttcat cccgagtcgc tgagcgtgcc cctgaataat
    45181 cgtaatcagc atctcatcca tcaaattacc ctgcacgttg gcgcataact caccatcaag
    45241 tagcaggtaa tctgccatat tcgctttcag cgagttgaag atttgcagat cgcggcccac
    45301 ctggctgagc actatccgac atcccaccag ccgcagtttt tgcacgcttt ctgcgtgatc
    45361 taaaatcgct tcagccggaa taatcagatg taataaccgt ggtggtagag ggctatttcc
    45421 cagctgctca agcagatcat tcaccagcgt ggcgctactc aaaccggcaa cggaaagggg
    45481 gagggcgatg cttatgcctt tactggcaac cgcttttgcg gcctgctgga aaaattcgtg
    45541 gaatacccgt cggtcaagag catggctaag tgccggatcg ctgaagctac gacgaaattt
    45601 ttgttcatca ataatctcgc cttcgcaact ccagagctta agtgaaatca gccacaaatt
    45661 acgcgcttcc gggatccgcg gcgaagcgac accgtgggcg atcatcatca actgattctc
    45721 tttaatcatc cgccactgtt catcaagcga catcgccgcc cgttcgctat gtgcggcagc
    45781 ttgctgtggt tcgtaaaccg tcacccggcc ccgtccacca tttttggagg cataacaggc
    45841 gatatcagcc tgcgacatca cttctgccgc ctgatgattg ttgtcatcaa tcaaggtaat
    45901 cccggcactg gcacctaccc gatgcacacg gccttcccat ataaagtgat aatcattcac
    45961 ggcactgata atgcgtgtag cgataaaacg cgcactttcg atattacagt ctggcaacag
    46021 tagaccaaat tcatcaccac cgagtcgcgc cagcacgtcg ctggagcgca gcatactcag
    46081 catcaacgac gccagttcgc gcagtaaagc gtcacccgcc gcatgcccgg cgctgtcatt
    46141 caccgcttta aagcgatcaa gatcgataaa caccagggca tgtcgctgat gtgtactgtt
    46201 taccgtttgc agcaggatac gcagttgctt ctcaaaactg gcgcgattgg cgagatgcgt
    46261 cagtgcatca tgggaggcgc tgtagctcag ctggcgcagc attttgcgtg attcggtgac
    46321 gtcctgaatc accagtaccg agcctatatt gctgccgtcc agagtactta acggcgtaat
    46381 actgtaatgc acgtcgtaac tgccgccgtt ccggcagtgc aacaccacat cttgttcaat
    46441 cgcggaacgt gaggtgtcgg cactgtaaat gttctccatt aatggtccgt tgtcgccaaa
    46501 agtaatatgc aacaccgtca ggagcggaac acctaatgct tcttcctgcg tccagccgct
    46561 catcttctcc gccactggat tcataaaggt aattttcatc gccatatcaa tacagaccac
    46621 ggcttcaccg atggaatcca gcgtaatgtg caggcgctct ttttcctgaa acaatgcctc
    46681 gttaagctgt ttaacctcgg tcatatccat attaatgccg agcaggcgtt cgacttcgcc
    46741 ttctttattc agtacccggt tggcgagggc gcggatatgg cgaataccgt cttttacggt
    46801 aatgcgaaat tccagcttaa agggcgagcg tgattgcaac gaatcacgaa tcactttttc
    46861 ggcatgctgg cgatcttccg gcagcacgca ctcgtaccac acctgccagt tcggtttgat
    46921 atgcggagga atttcataca gctcgaaaaa tattcggctt cagctcccac tcccagatgc
    46981 caatcccgcc cgcttcgtta gccagcgtga tgcgctccat cagttgctga ttcacctgtt
    47041 cggtgcgttt tagctcgtta atgtcttcaa tctgagcgat aaaatagagc ggcgtgccat
    47101 ccgtgtggcg caccagtgag acggcaagca acgcccagac aacatcgcca ttacggttgt
    47161 agtagcgttt ttccattgaa taggtgttta tttcaccgct tatcagcttt tcaacctgtt
    47221 ggagatcttt attgagatcc tccggccagg tcagttgctg aaaggtgagt ccgcgcagct
    47281 cttcctgact gtacccgaga aactggcaga gcgctttgtt ggtttgcagc cattgtccct
    47341 cggtgcccac taacgccata ccgatagcgg aatattccat cgcgttccga aagcgggttt
    47401 cgctttcgga aatgtgtttg cgttccgcac gaaaggcata catcaccatg gtcatgatgt
    47461 tggcgggcag caggatcagc aaaaacagta gccacggcat atggctcatc aggtacgtac
    47521 gcggcgtagc aagcagggag ggatccgcgg ccatcatcag cgacaccatc atcaccgtgg
    47581 taaggaagat caaaaaggct tccattcgtg gcaggcgcac ggcgctccac atcaacagca
    47641 caataatgaa agtaaaaggc cacggcaggt acaacataga aagccagctt aacgtcagcg
    47701 tgatggctaa tgtgagcagt gactcaaaaa gtaaccgtgg gttgcgatgg cgcagcagat
    47761 agtgtggctt aaataacaat cccagcggca ccagtaccag agcgccgatg gattctgaca
    47821 gtacccatat caaaaatgcc ctgagaggat cgtctccggg cgtcagcagg acaaccagaa
    47881 caccccctaa cagaggcgga acaatggcgc tgccgaatgc cagacgcagc cagtcagcca
    47941 gattttgcaa ggggttatac cacggcagca atttacgtag cagcactgcc ccgaccacgg
    48001 cttcaacaat attgatggtc gtccaggtca tgttcagcga gctggtggaa aaaagcagga
    48061 tggatgcggc gatatttccc agcgagcagc tcagcgcaat tcccggccac atgcgcccgg
    48121 catggcgata aaacgccacc atcatgatgg acgtcgggaa ccacagtggg gcgagttggg
    48181 tgccaaactg cgaaagctca agcgagaaaa gggtaaagat aaacgagact aaacctaaac
    48241 tgaccaggtg aagcagtggg tggggcaggg caattaatac atgctgtgat tgtttgctca
    48301 ttaccgcttt atccgcaaga ccaggtcgcc agtattcgtt tctgtgttta tgctagtaca
    48361 aaagttttac attttatatg tgaattgctc ataaagtgac gtaaaggcgg ataatttgcg
    48421 caactgcgta taacattttt taccttacat aaaactgatc aacgtaattt gccaccggga
    48481 attctttgtc aacaattaac tctgagcgcg cggcaacgct attcgactgg tatcaggcgg
    48541 atgaaatccc tataattgcc gcgtttggcg cttcgtcgcc cccttcctaa catccaggtt
    48601 aatcaggtcg ctaaatatat gactgatcag tctcatcagt gcgtcattat cggtatcgct
    48661 ggcgcatcgg cttccggcaa gagtcttatt gccagtaccc tttatcgtga attacgtgag
    48721 caagtcggtg atgaacacat cggcgtaatt cccgaagact gctattacaa agatcaaagc
    48781 catctgtcga tggaagaacg cgttaagacc aactacgacc atcccagcgc gatggatcac
    48841 aacctgctgc ttgagcattt acaagcgttg aaacgcggct cggcaattga cctgccggtt
    48901 tacagctatg ttgaacatac gcgtatgaaa gaaacggtga cggttgagcc gaagaaggtc
    48961 atcattctcg aaggcatttt gttgctgacg gatgcgcgtt tgcgtgacga acttaacttc
    49021 tccattttcg ttgatacccc gctggatatc tgcctgatgc gccgcatcaa gcgtgacgtt
    49081 aacgagcgtg ggcgttcaat ggattccgtg atggcgcaat atcaaaaaac cgtgcgcccg
    49141 atgttcctgc aattcattga gccttctaaa caatatgcgg acattatcgt gccgcgcggc
    49201 gggaaaaacc gcatcgcgat cgatatattg aaagcgaaaa taagtcagtt ctttgaataa
    49261 gcttgataaa ttgtgtaccg ttcagtgata acctggtatg cccttgacgt aattggcgtt
    49321 aagggagtga tgcgcgaaag gagaaaatgc catgcgtctg tgtgaccgag atattgaagc
    49381 ctggcttgat gaaggccgtt tgtcgatcaa cccacgtccg ccagtggagc gtattaacgg
    49441 cgcgacggtg gatgtacgcc tgggcaataa atttcgtacc ttccgtggtc acacggcagc
    49501 gtttatcgat ctgagcggtc ccaaagatga agtgagcgcc gcgcttgacc gcgtgatgag
    49561 cgatgagatc gttctcgacg agggcgaggc gttctatctt cacccaggag aactggcgct
    49621 ggcggtgacg ctggagtcgg tgacgctgcc agccgatctg gtgggctggc tggacgggcg
    49681 ttcctctttg gcgcgtctgg ggctgatggt gcacgtcacc gcgcaccgta tcgatccggg
    49741 ctggtctggt tgcattgtgc tggagttcta caactccggt aagctgccgc tggcgctgcg
    49801 tccgggcatg ttaattggtg cgctgagctt tgagccgctt tccggcccgg cggcgcgacc
    49861 ttacaaccgc cgtgaagatg cgaaatatcg caaccagcag ggcgcggtag ccagccgaat
    49921 cgataaagac taattcaggc ccattgagga taccatgaga cgatttctga cgacgctgat
    49981 gatactcctg gtcgtgctgg tggccgggtt atctgcgtta gtgttgctgg tgaatccgaa
    50041 tgatttccgc aactatatgg tcaagcaagt tgctgcacgt agcggttatc aattgcagct
    50101 cgacgggcca ctgcgttggc acgtctggcc gcagcttagt atcctctccg ggcgaatgtc
    50161 tctcaccgcc cagggcgcga gccagccact ggttcgcgcc gacaacatgc gtctggacgt
    50221 ggcgctttta ccactactga gtcatcaact gagcgttaag caggtgatgc taaaaggggc
    50281 agtgatccaa ctgacgccgc agacggaagt ggtgcgcagt gaagacgctc cggttgcacc
    50341 gcgcgacaat accttgccgg atctgtcaga cgatcgcgga tggtcgtttg atatatccag
    50401 tcttaaggtg gcggacagcg tgctggtgtt ccagcatgaa gatgacgagc aggtgacaat
    50461 ccgcaatatt cgcctgcaaa tggaacaaga tccccaacat cgtggttcat ttgagttctc
    50521 cgggcgggtt aatcgcgatc agcgcgatct cacgatatcc cttaacggta cggtagatgc
    50581 ttctgattat ccgcatgatt taacggcggc tattgaacaa attaactggc agttgcaggg
    50641 tgcagattta ccaaaacaag gtattcatgg gcaggggagt ttccaggccc agtggcagga
    50701 gtcacataaa cgcctttcat ttaaccaaat tagtttgacc gccaatgaca gtacgctgag
    50761 cgggcaagca caggtcacgc tgacagagaa accggaatgg cagctgagac tgcaattccc
    50821 gcaactgaat cttgacaacc tcatcccgct taatgaaaca gcgaatggtg aaaacggtgc
    50881 cgcgcagcag gggcagagcc aatcaacgtt gccgcgcccg gtcatttctt cgcgtattga
    50941 tgaaccggca tatcagggac tgcaaggctt tacggctgat attttgttgc aggccagtaa
    51001 cgtgcgctgg cgcggaatga attttacaga tgttgtcacg caaatgacca acaagtcggg
    51061 tttgctggaa attactcaac tgcagggcaa gcttaacggt ggacaggttt cactgccggg
    51121 cacgctggac gcgacatcaa taaatccgcg gataaacttc cagccacggc tggaaaacgt
    51181 tgagattggc accattctga aggcgtttaa ctatccgatt tcgttgaccg gaaagatgtc
    51241 actggctggt gatttctccg gtgctgacat agatgccgac gcattccgcc acaactggca
    51301 aggacaggca catgtcgaaa tgaccgacac gcgcatggaa gggatgaact tccagcagat
    51361 gattcagcaa gcggtagaac gtaatggtgg tgatgtgaag gccgctgaaa acttcgataa
    51421 cgtaacgcgt cttgaccgct ttaccaccga tttgacgttg aaggatggcg tcgtgacgtt
    51481 aaacgacatg caaggtcaat cgcctgtgct ggcgctttcc ggggctggta cgttgaatct
    51541 ggcggagcaa acctgcgaca cccagtttga tattcgggtc gtgggtggct ggaacgggga
    51601 aagcaaactg attgatttcc ttaaagaaac gccagtaccg ctgcgggttt atggcaactg
    51661 gcagcaactc aattacagtc tgcaagtgga tcagttactg cgcaaacatc tacaggacga
    51721 agcgaaacgt cgtctgaatg actgggccga gcggaataaa gattcccgca atggcaaaga
    51781 tgtgaagaag ttgctggaga agatgtaggt tacctggtga cctgtcctgt aggcctgata
    51841 agacgcgtaa agcgtcgcat caggcgttcg tggcggcatc attatattct gaatatgtaa
    51901 gatcgaaggc tgttccgttc acttgatgtt cctcgctact atggaacgct caagccggtg
    51961 aacgtgccag gggggctcaa tcgccaccac cctggcaaac cgggctcccg gcggtaaatc
    52021 gccgctggcg cggttccctc atcttattcc tttcggctat tgggtaaaat gaccgatacc
    52081 atcacacctc gtactccaac tcgccatcct tatgcagcgg aataatctgc accttctgca
    52141 cgcgatggct ttccacctgc aacgttttaa gcaaataatc ccccacctga acttcttcgc
    52201 caggttttgg aatacgctgt aaatattcca tcaacagccc ggcaatggtg tgatattcac
    52261 gtttttcatc cagcggcagc ggcacatatt gcaccagatc ttccagcggc atatgaccat
    52321 tcgccgtcca ggaaccgtcc gcattcttct gaatatcatg gcgggcgtcg atctcttcca
    52381 cttcgttcgg taagttaccg gcaatggttt cagtgacgtc acttaatgtc acaatccctt
    52441 ccaccgagcc aaactcatcc accacaaaag caaagtgtgt gcgggcatta cggaactgct
    52501 ccagggcagg taacaacggc aaggtttccg ggaacactag cggctggcga atcaacaccc
    52561 gcaggttgag cggttcgcca cgcagtgact gttgtagcag gtcgataacg tgaacaacac
    52621 cgagcaaatc ttctgcgtca tcaccgtcgg taaccaccag ccgcgtatgc tgatttcgct
    52681 ccagcaactg gcggatctcg tcttccggcg cgttgagatc gatatgctca atatcgtggc
    52741 gcgacgtcat aatgctgctg acagtacgct ggttaagatt aagtacccgc tcaatcatcc
    52801 gccgttcctg cggattaaag atctgctggt taccatgatc caccagcatg gacgcggttt
    52861 cggcgtccag ttccgcatct tctttttgcc cgctaagcag gcgcattacc gcttcggtag
    52921 tccgctggcg tagcgtctgg ttagcggaaa gaaaacgtcg ccggttaaag atagccaact
    52981 gattgagcgc ctcgatcatt accgagaaac caatggcagc gtacaggtag cctttcggaa
    53041 tgacgaagcc gaaaccttct gccaccaggc taaagccaat catcaacagg aagcttaagc
    53101 agagaataac gatggtcggg tgactgttaa caaattgcgt taacggcttg ctggccatca
    53161 acatcaggct gatagcgata accacggcgg ccatcatgac cagtaaatgg tcgaccatcc
    53221 cgacggcggt aatcactgag tcgagtgaga agatggcgtc cagtaccaca atttgtgtca
    53281 ccacccccca gaacttcgca cctttgcgtt gtgtgggatt attgctgtct ttcccttcca
    53341 gccgttcgtt cagctccatc gtggctttga acaacaggaa gaaaccacca aacagcatga
    53401 ttaagtcgcg ggcgctaaag gtaaacgagc ggaagctgaa cagcggttgc gtcagggtga
    53461 ccagccagga gattgacgcc agcagtaaca ggcgcattaa catcgccagc aacagcccgg
    53521 taacccgtgc gcggtcgcgc tgcttcggcg gtagtttttc ggcgaggatg gcaataaaga
    53581 ccaggttatc aatgccgagg accagttcga tcacaatcag cgtgattaac ccggcccaga
    53641 tagacggatc ggcaatccat tccatagtaa gtacaaaacc tattcgttgt atgacaaatg
    53701 tcacaattcg atcatggata aatcggggca aaaatgcaat gttgcagcac tatttatctc
    53761 atattatttg agggtaataa cctcacatta tccctgaatt aaaagtggta ataataaaac
    53821 attatttaag aaatatcgca tgcggaggcg caatattaaa tattgtcaac ctaaagaaac
    53881 tcctaaaaac catattgaat gacacttaat ataattctta aaaatagcca attgccgaat
    53941 tgttaccttg cctgctattc cgttagctgt aacacttcct cctgcattat tggagagcca
    54001 atattcaatt accataatgt caccttatat tcggaagtta atttccgcaa taaccctttc
    54061 tcatcaacga ttgtatgcag agagggttta aaaaattaag ctaaatcagt gtattggtgg
    54121 ctaaaaagcc aggggcggta gcgtgtctgg atgcctgaaa gaccagtctg aattattctg
    54181 tcaatagcct gcggatgaaa tcaatttttt taggactgat gccagttaaa tttgtattca
    54241 attaactgca aggtaaatgt gcatggcgac attgctatta atagtgcaca ggataattac
    54301 tctgccaaag tgataaataa acaatgatga aatccaaaat gaaattgatg ccattattgg
    54361 tgtcagtaac cttgataagc ggttgcacag tacttccggg cagcaatatg tcgacgatgg
    54421 gcaaagacgt catcaaacag caggacgctg atttcgatct cgacaaaatg gtgaatgttt
    54481 atccgctgac cccgcgcctg attgaccaat tacgcccacg cccgaatgta gcgcgcccca
    54541 atatgacgct ggaaagcgag atcgcgaatt accagtatcg cgtcgggccg ggggacgttc
    54601 ttaatgtcac cgtctgggat cacccggaac tcaccacgcc agctggtcag taccgcagct
    54661 ccagcgacac cggcaactgg gtacagcctg acggcactat gttttacccg tatatcggca
    54721 aggttcacgt agtcgggaaa acgctcgctg aaatccgcag tgatattacc gggcgcttag
    54781 cgacgtacat cgctgacccg caggtggacg ttaatatcgc cgccttccgc tcacaaaaga
    54841 cctatatctc aggtcaggtg aataaatccg gtcaacaggc gatcaccaac gtaccactga
    54901 ccattctcga cgccatcaac gccgcaggtg gcctgaccga caccgctgac tggcgcaacg
    54961 tggtgctaac acacaatggt cgtgaagaac gcatttcttt gcaggtgctg atgcaaaacg
    55021 gcgacctcaa tcagaaccga ctgctttatc ccggcgatat tctctacgtg ccgcgtaatg
    55081 atgatctgaa agtatttgtg atgggtgaag tgaagaaaca gagcaccctg aaaatggact
    55141 ttagcggcat gaccctgact gaagccctgg gtaatgctga aggtatcgac atgaccacct
    55201 ccaacgccag cggcatcttt gtcattcgtc cgctgaaagg cgagggcggg cgtaacggca
    55261 agattgccaa tatctaccag ctggatatgt ccgatgccac gtcgctggtg atggcgacag
    55321 aattccgcct gcaaccttat gacgtggtgt atgtcaccac cgccccggtt tcccgctgga
    55381 accgtctgat caatcagttg ctgccaacta ttagcggtgt ccgttacatg acggatacag
    55441 ccagcgacat tcataactgg taatcgtcat gtttaacaac atcttagttg tctgtgtcgg
    55501 caatatttgc cgctccccga cggcggaacg cttactgcaa cgttatcacc cggagctgaa
    55561 agtggagtcc gctggactcg gcgcgctggt cggtaagggc gctgacccta ctgctatcag
    55621 catcgccgca gaacatcaac tgtctctgga aggtcactgt gcacggcaaa tcagccgccg
    55681 tctgtgtcgc aactacgacc tgattttgac catggaaaag cgccatatcg aacgcttatg
    55741 cgagatggca cctgagatgc gcggcaaagt gatgctgttt ggtcactggg ataacgaatg
    55801 tgaaatcccc gatccgtatc gcaaaagccg ggaaacgttt gcagcggtgt acacattact
    55861 tgaacggtct gcccgccagt gggcgcaggc attgaacgca gagcaggtat aagaatgaca
    55921 gaaaaagtaa aacaacatgc cgctccggta acgggcagtg atgaaatcga tattggtcgc
    55981 ctggtcggca ccgtcattga agcgcgctgg tgggtgattg gcatcaccgc tgtatttgcc
    56041 ctctgtgccg tggtttacac cttcttcgcc acgccgattt atagtgccga cgcactggta
    56101 caaatcgagc aaaacagcgg caattcgtta gtgcaggaca ttggttcggc attagccaac
    56161 aaaccgcctg catcggacgc cgagatccag ttgattcgtt cgcgtctggt gcttggtaaa
    56221 acggtggacg atctcgacct cgatattgcg gtgagcaaaa acacgttccc gattttcggt
    56281 gcgggctggg atcgtttgat gggacgtcag aacgagacgg tgaaagtgac cacctttaac
    56341 cgcccgaaag agatggcgga tcaggtgttt acgcttaatg tgctggacga taaaaactac
    56401 accctgagca gtgatggcgg ctttagcgcc cgtggacaag cgggccagat gctgaaaaaa
    56461 gaaggcgtca cgctgatggt tgaagccatt cacgcccgcc cgggcagtga gtttaccgtc
    56521 accaaatact ccacgctggg gatgatcaat caactgcaaa acagcctgac ggtaacggag
    56581 aacggcaaag acgcaggcgt actgagcctg acttataccg gtgaagatcg cgaacagatc
    56641 cgcgacattc ttaacagcat cgcccgtaac tatcaggaac aaaatattga gcgcaaatcg
    56701 gcggaagcgt cgaaaagcct cgctttcctc gcgcaacagt taccggaagt acgtagccgc
    56761 cttgatgttg ccgaaaacaa actgaatgcc ttccgtcagg ataaagattc tgttgatctg
    56821 ccgctggaag cgaaagcggt gctcgattcg atggtgaaca tcgacgccca gttgaacgaa
    56881 ctgaccttta aagaggcgga aatctccaag ctgtacacca aagttcaccc ggcgtaccgc
    56941 acgctgctgg agaaacgtca ggcgctggaa gacgaaaaag ccaaacttaa cggtcgcgta
    57001 acggcgatgc cgaaaaccca gcaggagatt gtccgtctga cccgcgatgt cgagtctggt
    57061 cagcaggtct atatgcaact gctgaataaa gagcaggagc tgaaaatcac cgaggccagc
    57121 accgtcggcg atgtgcgcat tgttgacccg gcaatcaccc agccaggtgt actgaaaccg
    57181 aagaaagagc tgattatcct tggggcgatt atcctcggcc tgatgctttc tatcgtgggt
    57241 gtgctgctgc gctcgttgtt taatcgcggc atcgaaagcc cgcaggtgct ggaagaacac
    57301 ggtatcagcg tctatgccag catcccgctt tcggaatggc agaaagcgcg cgatagcgtc
    57361 aaaaccatca aagggattaa acgctataaa cagagccagc tactggcgat ggggaatcca
    57421 accgatctgg cgattgaagc catccgcagt ctgcgtacca gtttgcactt cgcgatgatg
    57481 caggcacaga acaatgtgtt gatgatgacc ggggttagcc cgtcaatcgg taaaaccttt
    57541 gtctgcgcca acctggcggc ggtgatcagc cagaccaata aacgggtgtt gttgatcgac
    57601 tgcgatatgc gcaaaggcta cacccacgag ctgctgggca ctaataacgt taatggcctg
    57661 tcggaaattc tgattggtca gggcgatatt actaccgctg ctaaaccgac ctctattgcc
    57721 aaatttgacc tgatcccgcg cggtcaggtg ccgccaaatc cttctgaact gttgatgagc
    57781 gaacgctttg ccgaactggt gaactgggcg agtaaaaact atgacctggt gttgattgat
    57841 acgccgccga ttctggcagt gaccgatgcg gcaattgttg gtcgtcatgt tggaaccacg
    57901 ttaatggtgg cgcgttatgc ggtcaacaca ttgaaagaag tggaaaccag tctgagccgc
    57961 tttgagcaaa atggtattcc ggtgaaaggg gtgattctga actccatctt ccgccgcgcc
    58021 agcgcgtatc aggattatgg ctattacgaa tacgaatata agtcggatgc gaaataacga
    58081 catccgctgt cggatgcgat gctgacgcat cttatccgac ctaccttcac gccgcatccg
    58141 gcaagcaaac cagctccgta ggccggataa ggcgctcacg ccgcatccgg caacagaacc
    58201 cgtaaacaag cttataaacc ggcagaacat ctcgacactc cgggagaaca acctatgaaa
    58261 gacaatccgc tgatttcaat ctatatgccg acctggaacc gccaacaact ggcgattcgg
    58321 gcgataaaat cggtcctgcg ccaggactac agcaactggg agatgatcat cgtggatgat
    58381 tgctccacct cctgggaaca actgcaacag tacgtcactg ccctcaacga tccgcgtatc
    58441 acctacattc acaacgacat taacagcggg gcgtgcgcgg tacgtaacca ggcgattatg
    58501 ctggcgcaag gggaatacat caccgggatt gatgacgatg acgaatggac acccaaccgt
    58561 ctgagcgtct tcctcgccca taaacagcaa ctggtcactc acgccttttt gtacgctaac
    58621 gactacgttt gccagggcga agtctattcc caaccagcaa gtttgccgct atatcccaaa
    58681 tcgccgtatt cacgccgcct gttctataaa cgcaatatca ttggcaatca ggtctttact
    58741 tgggcatggc gtttcaaaga gtgtttgttc gataccgaac tgaaagcagc acaggattac
    58801 gacatcttcc tgcggatggt ggtggagtac ggcgaaccgt ggaaagtgga agaagcgacg
    58861 cagatcctgc atatcaatca cggtgaaatg cagatcacct cgtcaccgaa aaaattctcc
    58921 gggtacttcc acttttaccg aaagcacaaa gataaatttg accgtgcaag caaaaaatat
    58981 cagctgttta ccctctacca gatccgtaat aaacgcatga cctggcgcac cttgttgacg
    59041 ctgctgtcgg tgcgtaacgg caaacgtctg gctgatggta ttcgggggcg ctaaagatgc
    59101 tggaagatct gcgcgccaac agctggagtt tacgcccgtg ctgcatggtt cttgcctatc
    59161 gtgttgctca tttttgttcg gtgtggcgca aaaagaacgt cctcaacaat ctgtgggcgg
    59221 caccgctgct ggtgctgtat cgcattatca ccgaatgctt tttcggttat gaaatccagg
    59281 cagccgcaac cattggccgc cgctttacta tccatcacgg ttacgccgta gtgatcaata
    59341 aaaacgtggt tgcgggggat gattttacca ttcgccacgg cgtcactatc ggcaatcgtg
    59401 gtgccgataa catggcatgt ccacacattg gcaacggcgt cgaactcggt gccaacgtca
    59461 ttattcttgg tgatatcacg cttggtaaca acgtcaccgt gggcgcgggc agcgtggtgc
    59521 tcgactctgt cccggacaac gcgctggtgg tgggagaaaa agcgcgagtg aaggtaatta
    59581 aatgaatatt ctgcaattta atgtgcgact ggcggaaggc ggagcggcag gtgtggcgtt
    59641 agatctccac cagcgtgcgc tgcaacaggg gctggcgtca cattttgtct acggctacgg
    59701 caaaggcggc aaagagagcg tcagccatca aaactatccg caggtaatca aacatacgcc
    59761 gcggatgacc gcgatggcga acattgcact gtttcgtctg tttaatcgcg atctgtttgg
    59821 caatttcaat gagttatatc gcaccattac tcgcacaccg ggtccggtgg tcctgcattt
    59881 tcatgtgctg cacagctact ggctaaatct taagagcgtg gtgcgctttt gcgaaaaagt
    59941 gaaaaaccat aaaccggacg tcactgtggt ctggacgctg cacgatcact ggagtgtaac
    60001 cgggcgctgc gcctttaccg acggttgcga aggctggaaa acgggttgcc agaaatgccc
    60061 gaccttaaat aactatccgc cggtgaagat tgatcgcgcg caccagctgg tggcgggcaa
    60121 acgccagtta tttcgtgaga tgctggcgct gggctgtcag tttatttccc ccagccagca
    60181 tgtggctgat gctttcaata gcctgtacgg tccagggcgt tgccggatta tcaataatgg
    60241 cattgatatg gcaaccgaag cgattctggc ggatctgcct ccggtgcgcg aaacccaggg
    60301 caagccgaaa atcgcggtgg tggcgcatga cctgcgttac gacggcaaaa ctaaccagca
    60361 actggtgcgt gagatgatgg cgctgggcga caaaatcgaa ctgcatacct ttggtaagtt
    60421 ctcgccgttc accgctggca acgtggttaa tcacggcttt gaaactgaca agcgcaagtt
    60481 gatgagcgcg ctcaatcaga tggatgcgct ggtgttcagt tctcgtgtcg ataactaccc
    60541 gctgattttg tgtgaggcgc tatcgattgg tgtgccggtg atcgccaccc atagcgatgc
    60601 ggcgcgggaa gtgctgcaaa aatccggagg aaaaactgtc agcgaagaag acgtgctgca
    60661 actggttcag ttaagcaaac cggaaatcgc gcaggcgata tttggtacca cgctggctga
    60721 gttcagccaa cgcagccgcg ccgcctacag tggacaacag atgctggagg agtatgtcaa
    60781 cttctatcag aatctgtagc tacctgctgc tgccgctgat ctacctgctg gttaacgtca
    60841 aaatcgccca gcttggtgaa agtttcccca tcactatcgt cactttttta cctgtcttgt
    60901 tactgctgtt tttagaacgc atcagcgtta aaaaattgat gattgcctta gggattggtg
    60961 cgggactcac ggcgttcaac tatctgtttg gtcagtcgtt ggatgccagt aaatacgtca
    61021 catcaaccat gctgtttgtc tatattgtga tcattattgg catggtgtgg agtattcgct
    61081 ttaaaacgat ttcgccacac aaccatcgca agatattacg tttcttttat ctggtggtcg
    61141 ggctggtggt ggcgctggcg gtggtggaga tggcgcaaat tatcctcacc ggtggcagca
    61201 gtattatgga gtcgatttcg aaatatctga tttacagcaa cagctatgta ctgaatttca
    61261 ttaaattcgg cggcaagcgc acgacagcac tttatttcga accggcattt ttcgctctgg
    61321 cattaatctc aatttggctc agcatcaaac agtttggtat caaaacgcct aaaaccgatg
    61381 ctatgattct cgcagggata atattatccg gatcgttttc aggggttatg acctttatcc
    61441 tgttttattt gctggagtgg gcatttcaat atctgaataa agaggcgatt aagaaaaagt
    61501 taccgttggc attgatttct ctggctgtat tcctggttgg tgtggtaatc gcgtttcctt
    61561 atatttccac ccgtctgggc gatttaggta cggaaggatc gtcatcgtat tatcgtattg
    61621 tcggtccgct ggtgatggtc ggttattctt tgacccatat tgacggtgta gtcagatttg
    61681 gctcacttta tgaatacgtc gcatcattcg gaatatttaa cggtgcggat gtcggaaaaa
    61741 ccatagacaa tggtttgtat ctgctgatta tttatttttc ctggttcgca gtgtttttat
    61801 cgctgtggta catggggaaa gtgataaaaa tgatgatcaa cgcttttggt gataaccgca
    61861 attttcgcgt gcaattatat ctttttactc cggtatcgct gttttttacc ggttcgatat
    61921 ttagcccgga atatgcattt ttaatcgtct gtccgtttat tttgcgaaaa gcgttaaata
    61981 ttacgaggta agaataagaa catgttgctt agcataatca ctgtcgcgtt tcgtaacctc
    62041 gaagggatag tcaaaacaca tgcctcgctg gcgcatctgg cgcaggcgga agatatcagc
    62101 ttcgaatgga ttgttgtcga tggcggttcc aatgacggca cccgtgagta tctggaaaat
    62161 ctcaatggta tctataacct acgctttgtc agcgagccag ataacggtat ctacgacgcc
    62221 atgaacaaag gcattacgat ggcacaaggc aagttcgcgt tgtttctcaa ttcgggcgat
    62281 atttttcatc aggatgccgc aaattttgtc cgtaagttaa aaatgcaaaa agataacatg
    62341 atgatcaccg gcgatgcgct gctggatttt ggcgacgggc ataaaattaa acgtagcgcc
    62401 aaaccgggct ggtatattta tcacagcctg cccgccagtc atcaggcgat atttttccct
    62461 gtatccggct tgaaaaaatg gcgttatgac ctggaatata aagtttcttc cgactacgcg
    62521 ctggcagcca aaatgtataa agccggttat gcatttaaaa aactcaatgg cctggtgtct
    62581 gaattttcca tgggtggggt atctaccacc aataatatgg aattgtgtgc tgacgcgaaa
    62641 aaagtccaac gacaaatatt acatgtacct ggcttttggg ctgaattatc ctggcattta
    62701 cgccaacgta ctacctcaaa gacgaaagcc ttatataaca aagtctgaat ataaggagaa
    62761 ccaatgcaag atttaagcgg tttctcggtg ccgaaagggt tccggggcgg caacgctatt
    62821 aaagtgcaat tatggtgggc agtacaggca acaatatttg cctggtcgcc acaagtattg
    62881 tatcgctggc gggcattttt attacgttta ttcggcgcaa aaataggaaa aaacgtagtt
    62941 attcgtccgt cagtaaaaat tacctatccg tggaaattaa ccttaggtga ttacgcgtgg
    63001 gtcggcgatg acgtcaattt atataccctc ggtgaaataa ccattggcgc acattcggtg
    63061 atatcgcaaa aaagttattt atgcaccggt agccacgacc atgcaagtca acatttcacc
    63121 attaatgcta cgcctattgt gattggcgag aaatgctggc tggcaaccga tgtttttgtt
    63181 gcccctggcg tcacgattgg cgacggcacc gtcgtgggtg cacgaagcag tgtttttaaa
    63241 acgcttccgg caaatgtggt ttgccggggg aatcccgcag tggtgatacg cgaacgcgtt
    63301 gaaactgaat aaattcaaaa aatacagagg aataagacat gtcaaaagtc gctctcatca
    63361 ccggtgtaac cggacaagac ggttcttacc tggcagagtt tctgctggaa aaaggttacg
    63421 aggtgcatgg tattaagcgt cgtgcatcgt cattcaacac cgagcgcgtg gatcacattt
    63481 atcaggatcc gcacacctgc aacccgaaat tccatctgca ttatggcgac ctgagtgata
    63541 cctccaacct gacacgcatt ttgcgtgaag tgcagccgga tgaagtttac aacctgggcg
    63601 caatgagcca cgttgcggtt tcttttgagt caccggaata taccgcagac gtcgatgcga
    63661 tgggtacgct gcgcctgctc gaggcgatcc gcttcctcgg tctggaaaag aaaacccgtt
    63721 tctatcaggc ttccacctct gaactgtatg gtctggtgca ggaaattccg cagaaagaaa
    63781 ccacgccgtt ctacccacgc tctccgtatg ctgtcgccaa actgtacgcc tactggatca
    63841 ccgttaacta ccgcgaatcc tacggcatgt acgcctgtaa cggtattctc ttcaaccatg
    63901 aatccccgcg ccgcggcgaa accttcgtta cccgcaaaat cacccgcgca atcgccaata
    63961 tcgcccaggg gctggagtcg tgcctgtacc tcggcaatat ggattccctg cgtgactggg
    64021 gccatgccaa agactacgta aaaatgcagt ggatgatgct gcaacaggaa cagccggaag
    64081 atttcgttat cgcgaccggc gttcagtact ccgtgcgtca gttcgtggaa atggcggcag
    64141 cacagctggg catcaaactg cgctttgaag gcacgggcgt tgaagagaag ggcattgtgg
    64201 tttccgtcac cgggcatgac gcgccgggcg ttaaaccggg tgatgtgatt atcgccgttg
    64261 atccgcgtta cttccgtccg gctgaagttg aaacgctgct cggcgacccg accaaagcgc
    64321 acgaaaaact gggctggaaa ccggaaatca ccctcagaga gatggtgtct gaaatggtgg
    64381 ctaatgacct cgaagcggcg aaaaaacact ctctgctgaa atctcacggc tacgacgtgg
    64441 cgatcgcgct ggagtcataa gcatgagtag acaacgcatt tttatcgctg gccatcgcgg
    64501 aatggtcggt tctgccatca ggcggcagct cgaacagcgc ggtgatgtgg aactggtatt
    64561 acgcacccgc gacgagctga acctgctgga cagccgcgcg gtgcatgatt tctttgccag
    64621 cgaacgcatt gaccaggtct atctggcggc ggcgaaagtg ggcggcattg ttgccaacaa
    64681 cacctatccg gcggatttca tctaccagaa catgatgatt gagagcaaca tcattcacgc
    64741 cgcgcatcag aacgacgtga acaaactgct gtttcttgga tcgtcctgta tctacccgaa
    64801 actggcaaaa cagccgatgg cagaaagcga gttattgcag ggcacgctgg agccgaccaa
    64861 cgagccttac gccattgcca aaatcgccgg gatcaaactg tgcgaatcat acaaccgcca
    64921 gtacggacgc gattaccgct cagtcatgcc gaccaacctg tatggcccgc atgacaactt
    64981 ccacccgagt aactcgcatg tgatcccagc attgctgcgt cgcttccacg aggcgacggc
    65041 acagaatgcg ccggacgtgg tggtatgggg cagcggtaca ccgatgcgtg aattcctgca
    65101 cgtcgatgac atggcggcgg cgagcattca tgtcatggag ctggcgcatg aagtctggct
    65161 ggagaacacc cagccgatgc tgtcgcacat taacgttggt acgggcgttg actgcactat
    65221 ccgcgagctg gcgcaaacca tcgccaaagt ggtgggttac aaaggtcggg tggtttttga
    65281 tgccagcaaa ccggatggta cgccgcgcaa actgctggat gtgacgcgcc tgcatcagct
    65341 tggctggtat cacgaaatct cactggaagc ggggcttgcc agcacttacc agtggttcct
    65401 tgagaatcaa gaccgctttc ggggggaatg atgtttttac gtcaggaaga ctttgccact
    65461 gtagtgcgct ccacaccgct tgtctctctc gactttattg tcgagaacag tcgcggcgag
    65521 tttctgcttg gcaaaagaac caaccgcccg gcgcagggtt actggtttgt gccgggaggg
    65581 cgcgtgcaga aagacgaaac gctggaagcc gcatttgagc ggctgacgat ggcggaactg
    65641 gggctgcgtc tgccgataac agcaggccag ttttacggtg tctggcagca cttttatgac
    65701 gataacttct ccggcacgga tttcaccact cactatgtgg tgctcggttt tcgcttcaga
    65761 gtagcggaag aagagctgtt aatgccggat gagcagcatg acgattaccg ctggctgacg
    65821 ccggacgcgc tgctcgccag taacgatgtt cacgctaaca gccgcgccta ttttctcgct
    65881 gagaagcgtg ccggagtacc cggattatga aaatactggt ctacggcatt aactactcgc
    65941 cggagttaac cggcatcggc aaatacaccg gcgagatggt ggaatggctg gcggcacaag
    66001 gtcatgaggt gcgggtcatt accgcaccgc cttactaccc gcaatggcag gtgggtgaga
    66061 actattccgc ctggcgctac aaacgagaag agggggccgc cacggtgtgg cgctgcccgc
    66121 tgtacgtgcc aaaacagccg agcaccctga aacgcttgtt gcatctcggc agttttgccg
    66181 tcagcagttt ctttccactg atggcgcaac gtcgctggaa gccggatcgc attatcggcg
    66241 tagtgccaac gctgttttgc acgccgggaa tgcgcctgct ggcgaagctc tctggtgcgc
    66301 gtaccgtgct gcatattcag gattacgaag tggacgccat gctggggctg ggccttgccg
    66361 gaaaaggcaa aggcggcaaa gtggcacagc tggcaacggc gttcgaacgt agcggactgc
    66421 ataacgtcga taacgtctcc acgatttcgc gttcgatgat gaataaagcc atcgaaaaag
    66481 gcgtggcggc ggaaaacgtc atcttcttcc ccaactggtc ggaaattgcc cgttttcagc
    66541 atgttgcaga tgccgatgtt gacgcccttc gtaaccagct tgacctgccg gataacaaaa
    66601 aaatcattct ttactccggc aatattggtg aaaagcaggg gctggaaaac gttattgaag
    66661 ctgccgatcg tctgcgcgat gaaccgctga tttttgccat tgtcgggcag ggcggcggca
    66721 aagcgcggct ggaaaaaatg gcgcagcagc gtggactgcg caacatgcaa tttttcccgc
    66781 tgcaatcgta tgacgcttta cccgcactgc tgaagatggg cgattgccat ctggtggtgc
    66841 aaaaacgcgg cgcggcagat gccgtattgc cgtcgaaact gaccaatatt ctggcggtag
    66901 gcggtaacgc ggtgattacc gcagaggccc acacagaact ggggcagctt tgcgaaacct
    66961 ttccgggcat tgcggtttgc gtagaaccgg aatcggtcga ggcgctggtg gcggggatcc
    67021 gtcaggcgct cctgctgccc aaacacaaca cggtggcacg tgaatatgcc gaacgcacac
    67081 tcgataaaga gaacgtgtta cgtcaattta taaatgatat tcggggataa ttatggcgca
    67141 gtcgaaactc tatccagttg tgatggcagg tggctccggt agccgcttat ggccgctttc
    67201 ccgcgtactt taccccaagc agtttttatg cctgaaaggc gatctcacca tgctgcaaac
    67261 caccatctgc cgcctgaacg gcgtggagtg cgaaagcccg gtggtgattt gcaatgagca
    67321 gcaccgcttt attgtcgcgg aacagctgcg tcaactgaac aaactcaccg agaacattat
    67381 tctcgaaccg gcagggcgta acactgcacc tgccattgca ctggcggcgc tggcggcaaa
    67441 acgtcatagc ccggagagcg acccgttaat gctggtattg gcggcggatc atgtgattgc
    67501 cgatgaagac gcgttccgtg ccgccgtgcg taatgccatg ccgtatgccg aagcgggcaa
    67561 gctggtgacc ttcggcattg tgccggatct acctgaaacc ggatatggct atattcgtcg
    67621 cggtgaagta tctgcgggtg agcaggatac ggtggccttt gaagtggcgc agtttgtcga
    67681 aaaaccgaat ctggaaaccg cccaggccta tgtggcaagc ggcgaatatt actggaacag
    67741 cggcatgttc ctgttccgcg ccggacgcta tctcgaagaa ctgaaaaaat atcgcccgga
    67801 tattctcgac gcctgtgaac aagcgatgag cgccgtcgat ccggatctcg attttattcg
    67861 cgtggatgaa gaagcgtttc tcgcctgccc ggaagagtcg gtggattacg cggtcatgga
    67921 acgtacggca gatgccgttg tggtgccgat ggatgcgggc tggagcgatg tcggttcctg
    67981 gtcttcatta tgggagatca gcgcccacac cgccgagggc aacgtttgcc acggtgatgt
    68041 gattaatcac aaaactgaaa acagctatgt gtacgccgaa tctggcctgg tcaccaccgt
    68101 cggggtgaaa gatctggtag tggtgcagac caaagatgcg gtgctgattg ccgaccgtaa
    68161 cgcggtgcag gatgtgaaaa aagtggtcga gcagatcaaa gccgatggtc gccatgagca
    68221 tcgggtgcat cgcgaagtgt atcgtccgtg gggcaaatat gactctatcg acgcgggcga
    68281 ccgctaccag gtgaaacgca tcaccgtgaa accgggcgag ggcttgtcgg tacagatgca
    68341 ccatcaccgc gcggaacact gggtagtggt cgcgggaacg gcaaaagtca ccattgatgg
    68401 tgatatcaaa ctgcttggtg aaaacgagtc catttatatt ccgctggggg cgacgcactg
    68461 cctggaaaac ccggggaaaa ttccgctcga tttaattgaa gtgcgctccg gctcttatct
    68521 cgaagaggat gatgtggtgc gcttcgcgga tcgctacgga cgggtgtaaa cgtcgcatca
    68581 ggagctgttt gtcggatgcg gcgtgaacgc cttatccgac ctacggttcg ggtttgtagt
    68641 cctgataaga cgcggcagcg tcgcatcagg caatgaatgc gaaaccgcgg tgtaaataac
    68701 gacaaaaata aaattggccg cttcggtcag ggccaactat tgcctgaaaa agggtaacga
    68761 tatgaaaaaa ttaacctgct ttaaagccta tgatattcgc ggaaaactag gcgaagaact
    68821 gaatgaagat atcgcctggc gcattggtcg cgcctatggc gaatttctca aaccgaaaac
    68881 cattgtgtta ggcggtgatg tccgcctcac cagcgaaacc ttaaaactgg cgctggcgag
    68941 aggtttacag gatgcgggcg tcgatgtgct ggatattggc atgtccggca ccgaagagat
    69001 ctatttcgcc acgttccatc tcggcgtgga tggcggcatt gaagttaccg ccagccataa
    69061 cccgatggat tataacggca tgaaactggt gcgcgaaggg gctcgcccga tcagcggtga
    69121 taccggactg cgcgacgtcc agcgtctggc tgaagccaac gactttcctc ccgtcgatga
    69181 aaccaaacgc ggccgctatc agcaaatcaa cctgcgtgac gcttacgttg atcacctgtt
    69241 cggttatatc aatgtcaaaa acctcacgcc gctcaagctg gtgattaact ccgggaatgg
    69301 cgcagcgggt ccggtggtag acgccattga agcccgcttt aaagccctcg gcgcgcccgt
    69361 ggaattaatc aaagtacaca acacaccgga cggcaatttc cccaacggta ttcctaaccc
    69421 gctgctgccg gaatgtcgcg acgacacccg caatgcggtc atcaaacacg gcgcggatat
    69481 gggcattgcc tttgatggcg attttgaccg ctgttttctg tttgacgaaa aagggcagtt
    69541 tatcgagggc tactacattg tcggcctgct ggcagaagcg ttcctcgaaa aaaatcccgg
    69601 cgcgaagatc atccatgatc cgcgtctctc ctggaacact attgatgtgg tgactgccgc
    69661 aggcggcacc ccggtaatgt cgaaaaccgg acacgccttt attaaagaac ggatgcgcaa
    69721 ggaagacgcc atctacggtg gcgaaatgag cgctcaccat tacttccgtg atttcgctta
    69781 ctgcgacagc ggcatgatcc cgtggctgct ggtagccgaa ctggtgtgcc tgaaaggaaa
    69841 aacgctgggc gaactggtgc gcgaccggat ggcggcgttt ccggcaagcg gtgagatcaa
    69901 cagcaaactg gcgcaacccg ttgaggcgat taatcgcgtc gaacagcatt ttagccgtga
    69961 ggcgctgtcg gtggatcgta ccgatggcat cagcatgacc tttgccgact ggcgctttaa
    70021 cctgcgcacc tccaacaccg aaccggtggt gcggttgaat gtggaatcgc gcggtgatgt
    70081 gccgctgatg gaagcgcgaa cgcgaactct gctgacgttg ctgaatgagt aatgtcggat
    70141 cttcccttac cccactgcgg gtaaggggct aataacagga acaacgatga caaatctaaa
    70201 aaagcgcgag cgagcgaaaa ccaatgcatc gttaatctct atggcgcaac gcttttcaga
    70261 tatcaccatc atgtttgccg gactatggct ggtttgcgaa gtgagcggac tgtcattcct
    70321 ctacatgcac ctgttggtgg cgttgattac gctggtggtg ttccagatgc tgggcggcat
    70381 caccgatttt tatcgctcat ggcgcggtgt tcgggcagcg acagaatttg ccctgttgct
    70441 acaaaactgg accttaagcg tgattttcag cgccggactg gtggcgttca acaatgattt
    70501 cgacacgcaa ctgaaaatct ggctggcgtg gtatgggctg accagtatcg gactggtggt
    70561 ttgccgttca tgtattcgca ttggggcggg ctggctgcgt aatcatggct ataacaagcg
    70621 tatggtcgcc gtagcgggag atttagccgc cggacaaatg ctgatggaga gcttccgtaa
    70681 tcagccgtgg ttagggtttg aagtggtggg ggtttaccac gacccgaaac cgggcggcgt
    70741 ttctaacgac tgggcgggca atctgcaaca gctggtcgag gatgcgaaag cgggcaaaat
    70801 tcataacgtc tatatcgcga tgcagatgtg cgacggcgcg cgagtgaaaa aactggtcca
    70861 tcaactggcg gataccacct gttcggtgct gctgatcccc gatgtcttta ccttcaacat
    70921 tctccattca cgtctcgaag agatgaacgg cgttccggtg gtgccgcttt acgacacgcc
    70981 gctttccggg gttaaccgcc tgctcaaacg tgcggaagac attgtgctgg cgacgcttat
    71041 tctgttgctg atctccccgg tgctgtgctg tattgcgctg gcggtgaaac tcagttcacc
    71101 agggccggtt attttccgcc agactcgcta tggcatggat ggcaagccga tcaaagtgtg
    71161 gaagtttcgt tccatgaaag tgatggagaa cgacaaagtg gtgacccagg cgacgcagaa
    71221 cgatccgcgc gtcaccaaag tggggaactt tctgcgccgc acctcgctgg atgaattgcc
    71281 gcagtttatc aatgtgctga ccggggggat gtcgattgtc ggtccacgtc cacacgcggt
    71341 ggcgcataac gaacagtatc gccagctcat tgaaggctac atgctgcgcc ataaggtgaa
    71401 accgggcatt accggctggg cgcagattaa tggctggcgt ggcgaaaccg acacgctgga
    71461 gaaaatggaa aaacgcgtcg agttcgacct tgagtacatc cgcgaatgga gcgtctggtt
    71521 cgatatcaaa atcgttttcc tgacggtatt caagggcttc gttaacaaag cggcatattg
    71581 atatgagctt acgtgaaaag accatcagcg gcgcaaagtg gtcggcgatt gctacggtga
    71641 tcatcatcgg cctcgggctg gtgcagatga ccgtgctggc gcggattatc gacaaccacc
    71701 agttcggttt gcttaccgtg tcgctggtga tcatcgcgct ggcagatacg ctttctgact
    71761 tcggtatcgc taactcgatt attcagcgaa aagaaatcag ccacctggaa ctcaccacgc
    71821 tgtactggct gaacgtcggg ctggggatcg tggtgtgtgt ggcggtgttt ttgttgagtg
    71881 atgccattgg tgacgtgctg aataacccgg acctggcacc gttgattaaa acattgtcgc
    71941 tggcgtttgt ggtgatcccc cacgggcaac agttccgcgc gttgatgcaa aaggaactgg
    72001 agttcaacaa aatcggcatg atcgaaacca gcgcggtgct ggcgggcttc acttttacgg
    72061 tggttagcgc ccatttctgg ccgctggcga tgaccgcgat cctcggttat ctggtcaata
    72121 gtgcggtaag aacgctgctg tttggctact ttggtcgaaa aatttatcgc cccggtctgc
    72181 atttctcgct ggcgtcagtt gcgccgaact tacgttttgg tgcctggctg acggcggaca
    72241 gcatcatcaa ctatctcaac accaaccttt caacgctggt gctggcgcgt attcttggcg
    72301 cgggcgtggc agggggatac aacctggcgt acaacgtggc cgttgtgccg ccgatgaagc
    72361 tgaacccaat catcacccgt gtgttgtttc cggcgtttgc caaaattcag gacgacaccg
    72421 aaaagctgcg cgtgaacttc tacaagctgc tgtcggtggt ggggattatc aactttccgg
    72481 cgctgctcgg gctgatggtg gtgtcgaata acttcgtacc gctggtattc ggcgaaaagt
    72541 ggaacagcat tattccggtg ctgcaattgc tgtgtattgt gggtctgctg cgctccgtgg
    72601 gtaacccgat tggttcgctg ctgatggcga aagcgcgggt cgatatcagc tttaaattca
    72661 acgtattcaa aacatttctg tttattccgg cgattgttat tggtgggcag atggcgggcg
    72721 cgatcggcgt cacgcttggc ttcctgctgg tgcaaattat caacaccatt ctgagttact
    72781 tcgtgatgat taaaccggtt cttggttcca gttatcgcca gtacatcctg agtttgtggc
    72841 tgccgtttta tctctcgttg ccgacgctgg tggtcagtta tgcgctgggc attgtgctga
    72901 aagggcaact ggcgctgggg atgctgctgg cggtgcaaat tatcgcaggc gtgctggcgt
    72961 ttgtggtgat gatcgtgttg tcgcgccatc cgctggtggt ggaagtgaag cgtcagtttt
    73021 gtcgcagcga aaaaatgaaa atgcttttgc gggccgggtg aatggttgtt ttcggttccg
    73081 gcaatagttg ccggatgcgg cgtgaacgcc ttatccggcc tacggctctg tgctcgggac
    73141 ttgtaggcct gataagacgc gccaggcaaa aagcggcaac gaacacaaaa aacacagcct
    73201 cacttggcat caaatttatg aggaaaaaat gaaattactg attctgggca accacacttg
    73261 cggcaatcgc ggcgacagcg ccatcttgcg cggcttactt gatgccatca acatcctcaa
    73321 tccacacgcc gaagtggacg taatgagccg ctatccggtc agttcttcct ggctgctcaa
    73381 ccgtccggta atgggcgatc cgctgttcct gcaaatgaaa caacacaaca gcgcggcggg
    73441 cgttgtcggg cgcgtaaaaa aagtcctccg tcgccgctac cagcaccagg tattgctctc
    73501 acgcgtcacc gacaccggca agctgcgtaa tatcgccatt gcccagggat tcactgactt
    73561 tgtgcgcctg ctgtcaggtt acgatgccat tatccaggtc ggcggatcgt tttttgtcga
    73621 tctctacggc gtgccgcagt ttgaacatgc gctttgcacg tttatggcga aaaagccgct
    73681 gtttatgatt ggtcacagcg tcggcccgtt ccaggatgag caatttaacc aactggcgaa
    73741 ctacgtattc ggtcactgcg acgcgctgat cctgcgcgaa tcggtcagcc tggatctaat
    73801 gaaacgcagc aatatcacta ccgcaaaagt tgaaaatggc gtcgataccg cgtggctggt
    73861 cgatcaccac attgaagact tcaccgccag ctatgctgtt caacactggc tggatgttgc
    73921 cgcacaacag aaagcggtgg caattaccct gcgcgaactg gcaccgttcg acaaacgtct
    73981 cggcaccact caagaagcgt atgaaaaagc ctttgccggg gtggtcaatc gcattctcga
    74041 cgaaggctat caggtgattg cgctctccac ctgtacgggt atcgacagct ataacaaaga
    74101 cgaccgcatg gtggcgctca acctgcgtca gcacatcagc gatcctgccc gttatcacgt
    74161 ggtgatggat gagctcaacg atctggaaat gggcaaaatc ctcggtgcct gtgaactcac
    74221 cgtaggtacg cgcctgcact ctgccattat ctcgatgaat ttctccactc cggcaattgc
    74281 catcaactac gaacacaaat ccgccgggat tatgcaacaa ctggggctgc cagagatggc
    74341 gattgatatt cgccatttac tggacggcag cctgcaagcg atggttgcgg ataccttagg
    74401 ccaacttccg gcgctgaacg cgcgacttaa cgaagccgtc agtcgtgagc gtcagacggg
    74461 aatgcggatg gtgcagtctg tgcttgagcg catcggggag gcgaaatgaa ggtcggcttc
    74521 ttcttattga aatttccgct gtcgtcggaa accttcgttc tcaaccaaat cactgcgttt
    74581 attgatatgg gctttgaggt ggagattgtc gcgctgcaaa aaggcgatac gcaaaacacc
    74641 cacgcggcat ggacgaaata taaccttgcc gccagaactc gctggttaca ggacgaacca
    74701 caaggcaaag tggcgaaact gcgctaccgc gccagccaga ccttacgcgg cattcatcgt
    74761 aaaaatacct ggcaggcgct caacctcaaa cgctatggtg ctgagtcgcg gaacctgatt
    74821 ttgtctgcca tttgcggcca ggtcgcaaca ccgtttcatg ctgatgtttt tatcgctcat
    74881 tttggccctg cgggggtaac cgcagcaaaa ctacgcgaac tggatgtcat tcgcggcaaa
    74941 attgccacta tcttccacgg tattgatatc tccagtcggg aagtgctcaa ccattacact
    75001 cccgaatatc aacaactgtt tcgtcgtggc gatttgatgc taccgataag cgaactgtgg
    75061 gccggaaggc tgcaaaaaat gggctgcccg agggaaaaaa ttgccgtatc gcgcatgggt
    75121 gtagacatga cgcgctttag cccgcgtccg gtaaaagcgc ctgcaacgcc gctggaaatc
    75181 atatctgtcg cacgcttaac cgagaaaaaa ggcctgcatg tggcgatcga agcctgccgg
    75241 cagttgaaag agcagggcgt ggcatttcgt tatcgcatcc tcggcattgg cccgtgggaa
    75301 cgacgcctgc gcacgctcat cgaacaatat caactggagg atgtggtaga gatgccgggc
    75361 tttaaaccaa gccacgaagt gaaggcgatg ctcgacgatg cggatgtctt tctgttgcca
    75421 tcggtaacgg gtgctgacgg cgatatggaa ggcattccgg tggcgctgat ggaagcgatg
    75481 gcggtcggca ttccggtggt ttctactctg catagcggaa taccggaact ggtggaggcc
    75541 gataaatccg gctggctggt gcctgagaac gatgctcgcg cactggcgca acgactggcg
    75601 gcgtttagcc aactggacac cgacgaactt actacggttg tcaaacgtgc gcgcgaaaaa
    75661 gtcgaacacg attttaacca gcaggtgatt aatcgagaac tcgccagctt gttgcaggct
    75721 ttatagagga gaacgcatgc catttaaaaa actctcccga cgcaccttcc tgacggcaag
    75781 ctcggcgctt gccttcctcc ataccccttt cgctcgcgca cttcccgccc gacaaagcgt
    75841 taacattaac gactacaacc ctcacgactg gatcgcctca tttaaacaag ccttcagcga
    75901 agggcaaacg gtcgtcgtgc ctggcggatt ggtttgcgac aatatcaaca ccggcatctt
    75961 cattcctgca ggtaaaacgt tacacatcct tggaagcctg cgcggcaacg gcagagggcg
    76021 atttgtttta caggacggca gccaggtgac aggggaggag ggcggcagta tgcataacat
    76081 caccctggat gtgcgtggtt ccgactgcac catcaaaggg ctgactatga gcggctttgg
    76141 cccggtaacg cagatttata tcggcggcaa aaacaaacag gtcatgcgca acctgaccat
    76201 cgataacctg accgttagcc acgctaatta cgccatctta cgccagggat ttcataacca
    76261 gattatcggt gccaacatca ccaattgtaa gttcagcgac ttacagggcg atgccattga
    76321 atggaacgtg gcgattaacg acagtgatat tttgatatct gaccacgtta ttgagcgcat
    76381 caactgtacc aacggcaaaa tcaactgggg catcggcata ggtcttgcgg gaagtaccta
    76441 cgataataat tatccggaag accaggcagt gaaaaacttt gtcgtggcga atatcacggg
    76501 atcggattgt cggcaactga tccatgttga aaatggtaaa cattttgtta ttcgtaatat
    76561 caaagcccgc aatatcacgc cggatttcag taagaaagca ggtattgata acgcgacagt
    76621 tgctatttac ggttgtgaca atttcgtgat tgataatatt gaaatgatta atagcgccgg
    76681 gatgttaatc ggctatgggg taattaaagg caaatatctc tcgataccgc aaaatttccg
    76741 agtgaataat attcaactgg ataacaccca tcttgcttat aaattgcgcg gcatccaaat
    76801 ctccgccggg aatgctgtct cctgtgtggc gctgactaac attgagatga agcgtgcgtc
    76861 gctggagtta cacaacaaac cacaacacct ttttatgcgt aatattaatg tgatgcagga
    76921 atcctctgtt ggaccagcat tgagcatgaa cttcgacatg cgcaaagacg ttcgcggcgt
    76981 ctttatggcg aaaaaagaaa cactactatc tcttgcaaat gttcatgcgg tgaatgaaag
    77041 agggcaaagc tccgtcgata tcgacaggat taatcaccat attgttaatg tggaaaagat
    77101 taactttaga ttgccggaac ggggggagta gatttgcgac cattcctgga aaaatggagc
    77161 catacttagg aacaatgcta ctgcaatcca caacgaagcg gcgtaacatc acaagtaatt
    77221 cagtaatcaa ttcagggtaa ttgatgctgg cgaaaaaaat cgaacaagct ataattcagc
    77281 aaccatttta caggtggatg aaataatgac gaatttaaaa gcagttattc ctgtagcggg
    77341 tcttgggatg catatgttgc ctgccactaa ggcgattccc aaagagatgc taccgatcgt
    77401 cgacaagcca atgattcagt acattgttga cgagattgtg gctgcaggga tcaaagaaat
    77461 cctcctggta actcacgcgt ccaagaacgc ggtcgaaaac cacttcgaca cctcttatga
    77521 attagaatct ctccttgaac agcgcgtaaa gcgtcaactg cttgcggaag tgcagtccat
    77581 ctgtccgccg ggcgtgacca ttatgaacgt gcgtcagggc gaacctttag gtttgggcca
    77641 ctccatttta tgtgcacgac ctgccattgg tgacaatcca tttgtcgtgg tgctgccaga
    77701 cgttgtgatc gacgacgcca gcgccgaccc gctgcgttac aaccttgctg ccatgattgc
    77761 acgtttcaat gaaacgggcc gcagtcaggt gctggcaaaa cgtatgccgg gtgacctctc
    77821 tgaatactcg gtcatccaga ccaaagaacc actggatcgc gaaggtaaag tcagccgcat
    77881 cgtcgaattt atcgaaaaac cggatcagcc gcagacgctg gactcagaca tcatggccgt
    77941 tggtcgctat gtgctttctg ccgatatttg gccggaactg gaacgtactc agcctggtgc
    78001 atggggacgt attcagctga ctgatgccat tgctgaactg gcgaaaaaac agtccgttga
    78061 tgccatgctg atgacaggtg acagctacga ctgcggtaaa aaaatgggct atatgcaagc
    78121 gttcgtgaag tatggactac gcaacctgaa agaaggggcg aagttccgta aagggattga
    78181 gaagttgtta agcgaataat gaaaatctga ccggatgtaa cggttgataa gaaaattata
    78241 acggcagtga agattcgtgg cgaaagtaat ttgttgcgaa tattcctgcc gttgttttat
    78301 ataaacaatc aggataacaa cgagttagca ataggatttt agtcaaagtt ttccaggatt
    78361 ttccttgttt ccagagcgga ttggtaagac aattagcgtt tgaatttttc gggtttagcg
    78421 cgagtgggta acgctcgtca catcgtagac atgcatgcag tgctctggta gctgtaaagc
    78481 caggggcggt agcgtgcatt aatacctcta ttaatcaaac tgagagccgc ttatttcaca
    78541 gcatgctctg aagtaatatg gaataataaa gtgaagatac ttgttactgg tggcgcagga
    78601 tttattggtt ctgctgtagt tcgtcacatt ataaataata cgcaggatag tgttgttaat
    78661 gtcgataaat taacgtacgc cggaaacctg gaatcacttg ctgatgtttc tgactctgaa
    78721 cgctatgttt ttgaacatgc tgatatttgc gatgctgctg caatggcgcg gatttttgct
    78781 cagcatcagc cggatgcagt gatgcacctg gctgctgaaa gccatgtgga tcgttcaatt
    78841 acaggccctg cggcatttat tgaaaccaat attgttggta cttatgtcct tttggaagcg
    78901 gctcgcaatt actggtctgc tcttgatggc gacaagaaaa atagcttccg ttttcatcat
    78961 atttctactg acgaagtcta tggtgatttg cctcatcctg acgaagtaaa taataaagaa
    79021 caattacccc tctttactga gacgacagct tacgcgccta gtagtcctta ttccgcatca
    79081 aaagcatcca gcgatcattt agtccgtgcg tggaaacgta cctatggttt accgaccatt
    79141 gtgactaact gttcgaataa ctacggtcct tatcactttc cggaaaaatt gattccacta
    79201 gtaattctta atgctctgga aggtaaggca ttacctattt atggcaaagg ggatcaaatt
    79261 cgtgactggc tgtatgttga agatcatgcg cgtgcgttat atatcgtcgt aaccgaaggt
    79321 aaagcgggtg aaacttataa cattggtgga cacaacgaaa agaaaaacat cgatgtagtg
    79381 ctcactattt gtgatttgtt ggatgagatt gtaccgaaag agaaatctta ccgtgagcaa
    79441 attacttatg ttgccgatcg cccgggacac gatcgccgtt atgcgattga tgcagagaag
    79501 attagccgcg aattgggctg gaaaccgcag gaaacgtttg agagcgggat tcgtaaaaca
    79561 gtggaatggt acctggctaa tgcaaaatgg gtcgagaatg tgaaaagtgg tgcttatcaa
    79621 tcgtggattg aacagaacta tgagggccgc cagtaatgaa tattctcctt ttcggcaaaa
    79681 cagggcaggt aggttgggaa ctacagcgtg ctctcgcacc tttgggtaat ttgattgctc
    79741 ttgatgttca ctccactgat tattgtggtg attttagtaa tcctgaaggt gtggctgaaa
    79801 ccgtcaaaaa aattcgccct gatgtaattg ttaatgctgc ggctcatacc gcagtagata
    79861 aggctgagtc agaacccgaa tttgcacaat tactcaatgc gaccagcgtt gaatcaattg
    79921 ccaaagcggc taatgaagtt ggggcctggg taattcatta ctcaactgac tacgtatttc
    79981 cgggaaccgg tgaaatacca tggctggaga cggatgcaac cgcaccgcta aatgtttacg
    80041 gtgaaaccaa gttagccgga gaaaaagcgt tacaggaaca ttgcgcgaag catcttattt
    80101 tccgtaccag ctgggtatac gcaggtaaag gaaataactt cgccaaaacg atgttgcgtc
    80161 tggcaaaaga gcgcgaagaa ctggctgtga ttaatgatca atttggtgcg ccaacaggtg
    80221 ctgagctgct ggcagattgt acggcacatg ctattcgtgt ggcactgaat aaaccagaag
    80281 tcgcaggcat gtaccatctg gtagccagtg gtaccacaac ctggcacgat tatgctgcgc
    80341 tggtttttga agaggcgcgc aaagcaggta ttccccttgc actcaacaag ctcaacgcag
    80401 taccagcaac agcctatcct acaccagctc gtcgtccaca taactctcgc cttaatacag
    80461 aaaaatttca gcagaacttt gcgcttgttt tgcctgactg gcaggttggc gtgaaacgaa
    80521 tgctcaacga attatttacg actacagcaa tttaatagtt tttgcatctt gttcgtgatg
    80581 atggagcaag atgaattaaa aggaatgatg aaatgaaaac gcgtaaaggt attattttag
    80641 cgggtggttc tggtacacgt ctttatcctg tgactatggc tgtcagtaaa cagctattac
    80701 ctatttatga taagccgatg atctattacc cgctctctac actgatgttg gcgggtattc
    80761 gcgatattct gattattagt acgccacagg atactcctcg ttttcaacaa ctgctgggtg
    80821 acggtagcca gtgggggcta aatcttcagt acaaagtgca accgactcca gatgggcttg
    80881 cgcaggcgtt tattatcggt gaagagttta tcggtggtga tgattgtgct ttggttcttg
    80941 gtgataatat cttctacggc cacgatctgc cgaagttaat ggataccgct gttgacaaag
    81001 aaagtggtgc aacggtattt gcctatcacg ttaatgatcc tgaacgctac ggtgtcgttg
    81061 agtttgataa aaacggtacg gcaataagcc tggaagaaaa accgctacaa ccaaaaagta
    81121 attatgcggt aaccgggctt tatttctatg ataacgacgt tgtcgaaatg gcgaaaaacc
    81181 ttaagccttc tgcccgtggt gaactggaaa ttaccgatat taaccgtatt tatatggaac
    81241 aggggcgttt atccgttgcc atgatggggc gtggttatgc gtggctggac acggggacac
    81301 atcaaagcct gattgaggca agcaacttca ttgctacaat tgaagaacgt caggggttga
    81361 aagtttcctg cccggaagaa attgcttacc gtaaagggtt tattgattct gagcaggtga
    81421 aaatattagc tgaaccactg aaaaaaaatg cttatggtca gtatctgcta aatatcataa
    81481 ataataaaat atcataatat taactagttg cagctatata aataggctcg gagattgcat
    81541 tgaagcttgt tacctctctt aataaaaata tcttctattt agctatattg caaggaagtt
    81601 attttatact gccattgtta acatttcctt atcttgttag agtattagga ccatactctt
    81661 ttggtctatt agggttttgt caagcaacaa tgcaatatct tgtgatggtg actgattatg
    81721 ggtttaattg gtcagcaaca caagatgttt cgaaaagtaa gaatgacaag gaaaaattaa
    81781 caacaatatt ttggaatgtc ttctttagta agattatact tgcactttcg gcaattgtta
    81841 tatttttcgg aataacattt tttattaagg
//
LOCUS       DAZPEB010000002        77378 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZPEB010000002 DAZPEB010000000
VERSION     DAZPEB010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 77378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77378
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.002"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..1624
                     /locus_tag="ACHURJ_000074"
     CDS             <1..1624
                     /locus_tag="ACHURJ_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR1279179.1"
                     /translation="NNGSQVNVTDSSLVSDTIGLTNGSSLNIGANGVVATDHLTVDSY
                     STVNLTESTGWNNYSNLYTNTITVTNGGVLDVNVDQFDTEAFRTDKLELTSGNIADHN
                     GNVISGVFDINSSDYVLNADLVNDRTWDTSKSNYGYGIVAMNSDGHLTINGNGDVDNG
                     TELDNSSVDNVVAATGNYKVRIDNATGAGTIADYKDKELIRVNDANTNATFSAANKAD
                     LGAYTYQAEQRGNTVVLQQMELTDYANMALSIPSANTNIWNLEQDTVGTRLTNSRHGL
                     ADNGGAWVSYFGGNFNGDNGTINYDQDVNGIMVGVDTKIDGNNAKWIVGAAAGFAKGD
                     MNDRSGQVDQDSQTAYIYSSAHFANNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNS
                     DAWGFGLKAGYDFKLGDAGYETPYGSVSGLFQSGDDYQLSNDMKVDGQSYDSMRYELG
                     VDAGYTFTYSEDQALTPYFKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSF
                     TKNFSAYTDANYLGGGDVDQDWSANVGVKYTW"
     gene            1712..2335
                     /gene="iprA"
                     /locus_tag="ACHURJ_000075"
     CDS             1712..2335
                     /gene="iprA"
                     /locus_tag="ACHURJ_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFR1279180.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNSEDT
                     FFILEGVISLRREENVLIGITQASYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINCLPSEY"
     gene            complement(2336..3493)
                     /gene="ampH"
                     /locus_tag="ACHURJ_000076"
     CDS             complement(2336..3493)
                     /gene="ampH"
                     /locus_tag="ACHURJ_000076"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFR1279181.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            3845..5065
                     /gene="sbmA"
                     /locus_tag="ACHURJ_000077"
     CDS             3845..5065
                     /gene="sbmA"
                     /locus_tag="ACHURJ_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFR1279182.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLVA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            5078..6172
                     /gene="yaiW"
                     /locus_tag="ACHURJ_000078"
     CDS             5078..6172
                     /gene="yaiW"
                     /locus_tag="ACHURJ_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFR1279183.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(6231..6539)
                     /locus_tag="ACHURJ_000079"
     CDS             complement(6231..6539)
                     /locus_tag="ACHURJ_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFR1279184.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            6799..7011
                     /locus_tag="ACHURJ_000080"
     CDS             6799..7011
                     /locus_tag="ACHURJ_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFR1279185.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            complement(7035..8129)
                     /gene="ddlA"
                     /locus_tag="ACHURJ_000081"
     CDS             complement(7035..8129)
                     /gene="ddlA"
                     /locus_tag="ACHURJ_000081"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFR1279186.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPSKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLRLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            8207..8364
                     /locus_tag="ACHURJ_000082"
                     /pseudo
     CDS             8207..8364
                     /locus_tag="ACHURJ_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8591..8851
                     /gene="iraP"
                     /locus_tag="ACHURJ_000083"
     CDS             8591..8851
                     /gene="iraP"
                     /locus_tag="ACHURJ_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFR1279187.1"
                     /translation="MKNLIAELLLKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIEQVEGALYEVKPDASIPDDDTELLRNYVKKLLKHPRQ"
     gene            8952..10367
                     /gene="phoA"
                     /locus_tag="ACHURJ_000084"
     CDS             8952..10367
                     /gene="phoA"
                     /locus_tag="ACHURJ_000084"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFR1279188.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNAVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            10486..10806
                     /gene="psiF"
                     /locus_tag="ACHURJ_000085"
     CDS             10486..10806
                     /gene="psiF"
                     /locus_tag="ACHURJ_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFR1279189.1"
                     /translation="MKITLLVTLFFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            10908..12023
                     /gene="adrA"
                     /locus_tag="ACHURJ_000086"
                     /gene_synonym="dgcC"
     CDS             10908..12023
                     /gene="adrA"
                     /locus_tag="ACHURJ_000086"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFR1279190.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(12040..12849)
                     /gene="proC"
                     /locus_tag="ACHURJ_000087"
     CDS             complement(12040..12849)
                     /gene="proC"
                     /locus_tag="ACHURJ_000087"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFR1279191.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPNKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            12969..13427
                     /locus_tag="ACHURJ_000088"
     CDS             12969..13427
                     /locus_tag="ACHURJ_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFR1279192.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            13610..14134
                     /gene="aroL"
                     /locus_tag="ACHURJ_000089"
     CDS             13610..14134
                     /gene="aroL"
                     /locus_tag="ACHURJ_000089"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFR1279193.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            14184..14375
                     /gene="yaiA"
                     /locus_tag="ACHURJ_000090"
     CDS             14184..14375
                     /gene="yaiA"
                     /locus_tag="ACHURJ_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFR1279194.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            14633..15310
                     /locus_tag="ACHURJ_000091"
     CDS             14633..15310
                     /locus_tag="ACHURJ_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFR1279195.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            15382..15666
                     /gene="ppnP"
                     /locus_tag="ACHURJ_000092"
     CDS             15382..15666
                     /gene="ppnP"
                     /locus_tag="ACHURJ_000092"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFR1279196.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            15814..16155
                     /locus_tag="ACHURJ_000093"
                     /pseudo
     CDS             15814..16155
                     /locus_tag="ACHURJ_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            16152..16235
                     /locus_tag="ACHURJ_000094"
     CDS             16152..16235
                     /locus_tag="ACHURJ_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1279197.1"
                     /translation="MTQRPWSKLQHKTHNIAALKIIVRRSE"
     gene            complement(16313..17224)
                     /gene="rdgC"
                     /locus_tag="ACHURJ_000095"
     CDS             complement(16313..17224)
                     /gene="rdgC"
                     /locus_tag="ACHURJ_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFR1279198.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            17349..18257
                     /gene="mak"
                     /locus_tag="ACHURJ_000096"
     CDS             17349..18257
                     /gene="mak"
                     /locus_tag="ACHURJ_000096"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFR1279199.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHISGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFAMDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            complement(18400..19584)
                     /gene="araJ"
                     /locus_tag="ACHURJ_000097"
     CDS             complement(18400..19584)
                     /gene="araJ"
                     /locus_tag="ACHURJ_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFR1279200.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMFFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADTPVLAKP
                     LG"
     gene            complement(19710..22847)
                     /gene="sbcC"
                     /locus_tag="ACHURJ_000098"
     CDS             complement(19710..22847)
                     /gene="sbcC"
                     /locus_tag="ACHURJ_000098"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFR1279201.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NGSLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVKAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHKRQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTTLQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALSDLVSHKT
                     RIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVKKI
                     NGLGYSKLESTFAVK"
     gene            complement(22844..24046)
                     /gene="sbcD"
                     /locus_tag="ACHURJ_000099"
     CDS             complement(22844..24046)
                     /gene="sbcD"
                     /locus_tag="ACHURJ_000099"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFR1279202.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALAESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            24236..24925
                     /gene="phoB"
                     /locus_tag="ACHURJ_000100"
     CDS             24236..24925
                     /gene="phoB"
                     /locus_tag="ACHURJ_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFR1279203.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            24983..26278
                     /gene="phoR"
                     /locus_tag="ACHURJ_000101"
     CDS             24983..26278
                     /gene="phoR"
                     /locus_tag="ACHURJ_000101"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFR1279204.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            26685..28004
                     /gene="brnQ"
                     /locus_tag="ACHURJ_000102"
     CDS             26685..28004
                     /gene="brnQ"
                     /locus_tag="ACHURJ_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFR1279205.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            28080..29453
                     /gene="proY"
                     /locus_tag="ACHURJ_000103"
     CDS             28080..29453
                     /gene="proY"
                     /locus_tag="ACHURJ_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFR1279206.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            29609..31331
                     /gene="malZ"
                     /locus_tag="ACHURJ_000104"
                     /pseudo
     CDS             29609..31331
                     /gene="malZ"
                     /locus_tag="ACHURJ_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
     gene            complement(31336..31917)
                     /gene="acpH"
                     /locus_tag="ACHURJ_000105"
     CDS             complement(31336..31917)
                     /gene="acpH"
                     /locus_tag="ACHURJ_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFR1279207.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRRETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFI
                     CYARKQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLNAHYTALGTRFWQFYPRMMAQASHKAL"
     gene            32010..33080
                     /gene="queA"
                     /locus_tag="ACHURJ_000106"
     CDS             32010..33080
                     /gene="queA"
                     /locus_tag="ACHURJ_000106"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFR1279208.1"
                     /translation="MRVTDFFFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDDRSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            33136..34263
                     /gene="tgt"
                     /locus_tag="ACHURJ_000107"
     CDS             33136..34263
                     /gene="tgt"
                     /locus_tag="ACHURJ_000107"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFR1279209.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            34286..34618
                     /gene="yajC"
                     /locus_tag="ACHURJ_000108"
     CDS             34286..34618
                     /gene="yajC"
                     /locus_tag="ACHURJ_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFR1279210.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            34646..36493
                     /gene="secD"
                     /locus_tag="ACHURJ_000109"
     CDS             34646..36493
                     /gene="secD"
                     /locus_tag="ACHURJ_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFR1279211.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            36504..37475
                     /gene="secF"
                     /locus_tag="ACHURJ_000110"
     CDS             36504..37475
                     /gene="secF"
                     /locus_tag="ACHURJ_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFR1279212.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLTQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            37604..37951
                     /gene="yajD"
                     /locus_tag="ACHURJ_000111"
     CDS             37604..37951
                     /gene="yajD"
                     /locus_tag="ACHURJ_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFR1279213.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(38128..39012)
                     /gene="tsx"
                     /locus_tag="ACHURJ_000112"
     CDS             complement(38128..39012)
                     /gene="tsx"
                     /locus_tag="ACHURJ_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFR1279214.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLNFGPFKEWYFANNYIYDMGRNKNGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(39311..39850)
                     /locus_tag="ACHURJ_000113"
     CDS             complement(39311..39850)
                     /locus_tag="ACHURJ_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFR1279215.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGGYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            40001..40450
                     /gene="nrdR"
                     /locus_tag="ACHURJ_000114"
     CDS             40001..40450
                     /gene="nrdR"
                     /locus_tag="ACHURJ_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFR1279216.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            40454..41557
                     /gene="ribD"
                     /locus_tag="ACHURJ_000115"
     CDS             40454..41557
                     /gene="ribD"
                     /locus_tag="ACHURJ_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFR1279217.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVASMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHHIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWGEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            41646..42116
                     /gene="ribE"
                     /locus_tag="ACHURJ_000116"
     CDS             41646..42116
                     /gene="ribE"
                     /locus_tag="ACHURJ_000116"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFR1279218.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            42136..42555
                     /gene="nusB"
                     /locus_tag="ACHURJ_000117"
     CDS             42136..42555
                     /gene="nusB"
                     /locus_tag="ACHURJ_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFR1279219.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            42633..43610
                     /gene="thiL"
                     /locus_tag="ACHURJ_000118"
     CDS             42633..43610
                     /gene="thiL"
                     /locus_tag="ACHURJ_000118"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFR1279220.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGVRIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDHFATP"
     gene            43588..44106
                     /gene="pgpA"
                     /locus_tag="ACHURJ_000119"
     CDS             43588..44106
                     /gene="pgpA"
                     /locus_tag="ACHURJ_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFR1279221.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(44160..45134)
                     /gene="yajO"
                     /locus_tag="ACHURJ_000120"
     CDS             complement(44160..45134)
                     /gene="yajO"
                     /locus_tag="ACHURJ_000120"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFR1279222.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLFYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            complement(45189..47051)
                     /gene="dxs"
                     /locus_tag="ACHURJ_000121"
     CDS             complement(45189..47051)
                     /gene="dxs"
                     /locus_tag="ACHURJ_000121"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFR1279223.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            complement(47076..47975)
                     /gene="ispA"
                     /locus_tag="ACHURJ_000122"
     CDS             complement(47076..47975)
                     /gene="ispA"
                     /locus_tag="ACHURJ_000122"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFR1279224.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSPDTSALEALADYIIQRNK"
     gene            complement(47975..48217)
                     /gene="xseB"
                     /locus_tag="ACHURJ_000123"
     CDS             complement(47975..48217)
                     /gene="xseB"
                     /locus_tag="ACHURJ_000123"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFR1279225.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            48423..49871
                     /gene="thiI"
                     /locus_tag="ACHURJ_000124"
     CDS             48423..49871
                     /gene="thiI"
                     /locus_tag="ACHURJ_000124"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFR1279226.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIALQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            complement(49925..50515)
                     /gene="yajL"
                     /locus_tag="ACHURJ_000125"
     CDS             complement(49925..50515)
                     /gene="yajL"
                     /locus_tag="ACHURJ_000125"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFR1279227.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLQVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(50478..51389)
                     /gene="panE"
                     /locus_tag="ACHURJ_000126"
     CDS             complement(50478..51389)
                     /gene="panE"
                     /locus_tag="ACHURJ_000126"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFR1279228.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSANLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            51557..52048
                     /gene="yajQ"
                     /locus_tag="ACHURJ_000127"
     CDS             51557..52048
                     /gene="yajQ"
                     /locus_tag="ACHURJ_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFR1279229.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            complement(52176..53540)
                     /locus_tag="ACHURJ_000128"
     CDS             complement(52176..53540)
                     /locus_tag="ACHURJ_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1279230.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPA
                     YVSSLRIEIPANIAANEALKVRLLETEGIKEVLIAEEEHSAYVKIDSKVTNRFDVEQA
                     IRQA"
     gene            complement(53689..54579)
                     /gene="cyoE"
                     /locus_tag="ACHURJ_000129"
     CDS             complement(53689..54579)
                     /gene="cyoE"
                     /locus_tag="ACHURJ_000129"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFR1279231.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(54591..54920)
                     /locus_tag="ACHURJ_000130"
     CDS             complement(54591..54920)
                     /locus_tag="ACHURJ_000130"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFR1279232.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(54920..55534)
                     /locus_tag="ACHURJ_000131"
     CDS             complement(54920..55534)
                     /locus_tag="ACHURJ_000131"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFR1279233.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTCLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(55524..57515)
                     /gene="cyoB"
                     /locus_tag="ACHURJ_000132"
     CDS             complement(55524..57515)
                     /gene="cyoB"
                     /locus_tag="ACHURJ_000132"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFR1279234.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWANSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(57537..58484)
                     /gene="cyoA"
                     /locus_tag="ACHURJ_000133"
     CDS             complement(57537..58484)
                     /gene="cyoA"
                     /locus_tag="ACHURJ_000133"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFR1279235.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            complement(58945..60420)
                     /gene="ampG"
                     /locus_tag="ACHURJ_000134"
     CDS             complement(58945..60420)
                     /gene="ampG"
                     /locus_tag="ACHURJ_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFR1279236.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(60464..61042)
                     /locus_tag="ACHURJ_000135"
     CDS             complement(60464..61042)
                     /locus_tag="ACHURJ_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR1279237.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLAASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKITKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            61347..61664
                     /gene="bolA"
                     /locus_tag="ACHURJ_000136"
     CDS             61347..61664
                     /gene="bolA"
                     /locus_tag="ACHURJ_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFR1279238.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            62008..63306
                     /gene="tig"
                     /locus_tag="ACHURJ_000137"
     CDS             62008..63306
                     /gene="tig"
                     /locus_tag="ACHURJ_000137"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFR1279239.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDSAVEA
                     EDRVTIDFTGSVDSEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            63551..64174
                     /gene="clpP"
                     /locus_tag="ACHURJ_000138"
     CDS             63551..64174
                     /gene="clpP"
                     /locus_tag="ACHURJ_000138"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFR1279240.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            64300..65574
                     /gene="clpX"
                     /locus_tag="ACHURJ_000139"
     CDS             64300..65574
                     /gene="clpX"
                     /locus_tag="ACHURJ_000139"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFR1279241.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            65762..68116
                     /gene="lon"
                     /locus_tag="ACHURJ_000140"
     CDS             65762..68116
                     /gene="lon"
                     /locus_tag="ACHURJ_000140"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFR1279242.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENCVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            68307..68597
                     /gene="hupB"
                     /locus_tag="ACHURJ_000141"
     CDS             68307..68597
                     /gene="hupB"
                     /locus_tag="ACHURJ_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFR1279243.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            68789..70660
                     /gene="ppiD"
                     /locus_tag="ACHURJ_000142"
     CDS             68789..70660
                     /gene="ppiD"
                     /locus_tag="ACHURJ_000142"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFR1279244.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            70811..71182
                     /locus_tag="ACHURJ_000143"
     CDS             70811..71182
                     /locus_tag="ACHURJ_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFR1279245.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            71276..71674
                     /gene="fadM"
                     /locus_tag="ACHURJ_000144"
     CDS             71276..71674
                     /gene="fadM"
                     /locus_tag="ACHURJ_000144"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFR1279246.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(71726..72421)
                     /gene="queC"
                     /locus_tag="ACHURJ_000145"
     CDS             complement(71726..72421)
                     /gene="queC"
                     /locus_tag="ACHURJ_000145"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFR1279247.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(72486..74186)
                     /locus_tag="ACHURJ_000146"
     CDS             complement(72486..74186)
                     /locus_tag="ACHURJ_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFR1279248.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            74286..75068
                     /gene="cof"
                     /locus_tag="ACHURJ_000147"
     CDS             74286..75068
                     /gene="cof"
                     /locus_tag="ACHURJ_000147"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFR1279249.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTH"
     gene            75257..75715
                     /gene="decR"
                     /locus_tag="ACHURJ_000148"
     CDS             75257..75715
                     /gene="decR"
                     /locus_tag="ACHURJ_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFR1279250.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            75745..>77378
                     /locus_tag="ACHURJ_000149"
     CDS             75745..>77378
                     /locus_tag="ACHURJ_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFR1279251.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYFLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASENVMNAS"
BASE COUNT        18894 a        19172 c        20574 g        18738 t
ORIGIN      
        1 aaataatggc tctcaggtaa atgttactga ttcctctctg gtatcagata ccattggttt
       61 aaccaacggt tcttcactga atattggtgc aaatggcgtg gttgctacag accacctgac
      121 tgttgatagc tacagtaccg ttaatttaac cgaatctacc ggttggaata attattctaa
      181 tctgtatacc aatactatca ctgtgactaa cggtggtgtg ctggatgtga atgttgatca
      241 gttcgatact gaagctttcc gtactgataa actggaactg accagcggca acattgctga
      301 ccataatggc aacgtaattt ctggtgtgtt cgacatcaac agcagcgatt acgttctgaa
      361 cgctgatctg gtgaacgacc gtacgtggga tacttccaag tctaactacg gttacggtat
      421 tgttgctatg aactctgatg gtcacctgac tatcaatggt aacggcgacg tagacaacgg
      481 tactgaactg gataacagct ctgttgataa cgttgttgct gcaaccggta actacaaagt
      541 tcgtatcgac aacgcaactg gcgctggcac tatcgctgat tacaaagata aagaacttat
      601 tcgggtaaac gacgctaaca ccaacgcgac cttctctgct gctaacaaag ctgacctggg
      661 tgcatacacc tatcaggctg aacagcgcgg taacaccgtt gttctgcaac agatggagct
      721 gaccgactac gctaacatgg cgctgagcat cccgtctgcg aacaccaata tctggaacct
      781 ggaacaagac accgttggta ctcgtctgac caactctcgt catggcctgg ctgataacgg
      841 cggcgcatgg gtaagctact tcggtggtaa cttcaacggc gacaacggca ccatcaacta
      901 tgatcaggat gttaacggca tcatggtcgg tgttgatacc aaaattgacg gtaacaacgc
      961 taagtggatc gtcggtgcgg ctgcaggctt cgctaaaggt gacatgaatg accgttctgg
     1021 tcaggtggat caagacagcc agactgccta catctactct tctgctcact tcgcgaacaa
     1081 cgtctttgtt gatggtagct tgagctactc tcacttcaac aacgacctgt ctgcaaccat
     1141 gagcaacggt acttacgttg acggtagcac caactctgac gcgtggggct tcggtttgaa
     1201 agccggttac gacttcaaac tgggtgatgc tggttacgag actccttacg gcagcgtttc
     1261 tggtctgttc cagtctggtg atgactacca gctgagcaac gacatgaaag ttgacggtca
     1321 gtcttacgac agcatgcgtt atgaactggg tgtagatgca ggttatacct tcacctacag
     1381 cgaagatcag gctctgactc cgtacttcaa actggcttac gtctacgacg actcaaacaa
     1441 cgataacgat gtgaacggtg attccatcga taacggtact gaagggtctg cggtacgtgt
     1501 tggtctgggt actcagttca gcttcaccaa gaacttcagc gcctataccg atgctaacta
     1561 cctcggtggt ggtgacgtag atcaagactg gtccgcgaac gtgggtgtta aatatacttg
     1621 gtaatattct tcactccgaa gaaattctgg taatttaatc taaataatgc ccgtcaagga
     1681 tttgacgggc attactgcaa aggacgcgca aatgttatct gtagttaaac ctcttcagga
     1741 atttggtaag ctcgataaat gtttgtccag atacggtacg cgcttcgagt ttaataatga
     1801 aaagcaagtt atattttcca gtgatgtcaa tagcgaagat actttcttta ttttagaggg
     1861 cgttatctct ctgcgtagag aagaaaacgt acttatcggt attacccagg cttcttatat
     1921 tatggggctg gctgatggtt taatgaaaaa cgatatacca tacaaattaa tatcagaagg
     1981 aaattgtacg ggatatcatc taccagccaa acaaaccatt acgcttattg aacaaaatca
     2041 actctggcga gacgcttttt actggttagc ctggcaaaat agaattctgg aattacgcga
     2101 cgtgcagctc attgggcata attcctacga acaaatccgc gcaacattat tatcaatgat
     2161 tgactggaat gaagaattgc gatcacgtat tggtgtgatg aattatatcc atcaacgtac
     2221 acgcatatcg cgttctgtcg tcgcagaagt tctcgctgct ttgcgtaaag gcggctatat
     2281 cgaaatgaat aaaggcaaac tggtcgctat caactgtttg ccttcagagt attaatcagg
     2341 atgcagggat aaccaacggt ttattcccgc ttagctcggt caccagatca ttgatgccat
     2401 cgctcatatt tttaaagcgc gtcaacggcg aacgagtgac caccacaaac gcgccaatat
     2461 tcttctgcgg gatcatcgcc atataggtaa tgaaaccgcc accaccgcct gtcttctgaa
     2521 taatccccgg acgaccctct ttcggggcca tatataccca gcctaaacca agcgcatccg
     2581 ctttgccggg gacatccatg ccaatcactt tggtaaactg cgcgcgctga taaatcaacg
     2641 tctgcatgcg atctgcctgg ttactgcgct gataaaaatc agatgataaa tactgctgca
     2701 tccagcgcat catatcgcca ggcgtggaat agacgccgcc gctgccaatt gccgccagcg
     2761 tgttattgca cggactggca ccgcgctcag caaccattaa acggcggcac tgatccggtg
     2821 agggggtgta ggtggtgtct ttcatcccca atggacgggt aatctgctct tcaaacaact
     2881 gggtataagg cttgccggac gcattcgcca gtgcatctgc cagcaggtcg aacgcaaggt
     2941 tagagtacgc cgcctgactg cctggcgcgg ctttcagctt cgccgtagaa aggtatttcc
     3001 agcgttgctc gcgcgttggc cagacaaaca ccggacgatg tgccgcgcca ccgggctgtt
     3061 cacggggcag ggcgctggta tgggttgcca gattcactag ggtaatgggc gtcccattgt
     3121 aggttggcac gcgtgcgcct ggcggagcat atttacttag cggatcgttt aacttcacgg
     3181 tcccctggtc gagcaatttc accagcattt cactggtcat tagcttggtg agggaagcaa
     3241 tacgcacgac cgaatccagc tgcgggcgaa cgttattacc aggtcgcgtt tcgccataac
     3301 tgcgaaagac gcgctggtta ccgtcgataa caaccagcgc catccccgtg gcaccgctgc
     3361 cgtaaaaaat atgatcggca taacgatcga caatatcaga ggcaaactcc ggttcagtga
     3421 tcggctgcgc cgcatggacg gaggtcaatg acgccgcaca cagcacggca gaaaaaagca
     3481 gactacgttt caacggtggt gtccatagat tgaatgaagg taactgagcg tatttatact
     3541 actcattgat ggattgcaaa gggcgaatta gtgcaataat agtgttaaaa acgtaacggc
     3601 gcgtaaacac gatctttttc ctgctcacca atgcctgcat ttgtgatcct gcgcgtgtta
     3661 atccgcttat gtaaacaatc tttggtttgt gaaatccgtc gaaacaattc ttatggtcag
     3721 gctggcgaac taagcgcctt gctatgggtc acaatgggcg gtttcatttg gctgacgctt
     3781 tgtagtggtc atgcggttaa tacacagcag ttgttaaaac gataagaagt tagcaggagt
     3841 gcatatgttt aagtcttttt tcccaaagcc gggagcgttt tttctctcgg cctttgtttg
     3901 ggcattgatt gccgttatct tctggcaagc cggtggggga gactgggtgg cgcgtatcac
     3961 cggcgcttcc gggcagatcc cgattagcgc cgcgcgtttc tggtcgttgg atttcctgat
     4021 tttttacgct tactacattg tttgcgtagg actttttgca tttttctggt ttatctacag
     4081 cccgcaccgt tggcaatact ggtcaatact cggtactgca ctgatcatct tcgtcacctg
     4141 gtttttggtg gaagtcggag tcgccgtcaa cgcctggtat gcaccgttct atgatctgat
     4201 tcaaaccgcg ctaagttcgc cgcataaagt cactatcgaa caattttacc gcgaagtggg
     4261 cgtctttctg gggattgcgc tgatcgcggt ggtgatcagt gtactgaaca acttctttgt
     4321 cagtcactat gtgttccgct ggcgtacggc gatgaacgaa tattacatgg cgaactggca
     4381 acaactgcgt catatcgaag gtgccgcaca gcgtgtgcag gaagacacca tgcgttttgc
     4441 ttcaacgctg gagaatatgg gcgtcagttt tatcaacgcc atcatgacgt tggtcgcctt
     4501 cctgccggtg ctggtaacgc tctccgcgca tgtgccggag ctgccgatta tcgggcacat
     4561 tccgtatggt ctggtgattg ccgctatcgt ctggtcgctg atggggaccg gattgctggc
     4621 agtggtaggg atcaaactgc cggggctgga gtttaaaaac cagcgagtag aggccgccta
     4681 ccgtaaagag ctggtttatg gtgaagacga tgccacgcgc gcgacgccgc ctacggtacg
     4741 cgagctgttt agcgccgtac ggaaaaacta ttttcgcctc tattttcact atatgtattt
     4801 caacatcgcc cgcattctct atttgcaggt cgataacgtt ttcggtttgt tcttgctgtt
     4861 tccgtcaatt gttgccggta cgattacgct cggcctgatg acgcagatta ccaacgtttt
     4921 tggtcaggtt cgcggtgctt tccagtacct gattaactca tggaccacac tggttgagtt
     4981 gatgtctatc tacaaacgtc tgcgcagctt tgaacatgag ctggatggtg acaaaattca
     5041 ggaagtaacc cataccttga gctaaaaagg agaagtaatg tcgcgcgtca atcatctatc
     5101 ttcgctgtca ttgttggcgg ttctggtttt agcggggtgt agtagtcagg ctccgcaacc
     5161 gttgaaaaag ggcgaaaaag cgatagatgt agcgagcgtc gtgcggcaga agatgcctgc
     5221 gagcgtgaaa gaccgcgatg cctgggcgaa agatctcgcc accacctttg aaagtcaggg
     5281 gctggcaccg acgctggaaa acgtctgctc ggtgctggcg gtggcgcagc aggagtcaaa
     5341 ttatcaggca gatccggccg ttccgggctt aagcaagatc gcctggcaag agattgaccg
     5401 tcgtgctgaa cggatgcaca ttcctgcttt tctggttcat accgcgctaa aaatcaaatc
     5461 gccaaacggc aaaagttata gcgaacggtt agattcggtg cgtacagaaa agcaattgag
     5521 cgcgattttt gacgatctga tcagcatggt accaatgggg cagacgctgt ttggttcgct
     5581 taatccggtg cgcaccggtg ggccgatgca ggtcagcatt gcttttgccg aacagcatac
     5641 caaagggtat ccgtggaaaa tggacggtac agttcgtcag gaagtcttca gtcgccgcgg
     5701 cgggttgtgg tttggtactt accatttact gaattatccc gccagctata gcgcaccgat
     5761 ataccgcttt gctgatttta acgctggctg gtacgccagc cgtaatgccg cgtttcagaa
     5821 cgcggtcagt aaggccagcg gcgtgaagct ggcactggac ggtgatttaa ttcgttacga
     5881 cagtaaagaa cccgggaaaa ccgaactggc aacgcgcaaa ctggcgggaa aactggggat
     5941 gagcgacagc gaaattcgcc gtcagttaga gaaaggtgac agcttctctt ttgaggagac
     6001 ggcgctgtac aagaaagttt atcaacttgc cgaagcgaaa acgggtaaat cactaccccg
     6061 cgaaatgttg cctggcattc aactggaaag cccgaaaatc acccgcaacc tgactacggc
     6121 ctggtttgcg aagcgcgtag acgaacggcg ggcgcgttgt atgaaacagt gatcaaacag
     6181 gaatgtcatg ccagataagg cgttttaggc cgtatctggc aacgtaaatc ttactggcgg
     6241 cgatgtcgcc agtgcagcca tagcgccacc actgcaaaaa tgaggcagcc tactaaaaac
     6301 gggatcagcc cgaaaatggt gccgacacct aaaccaattt ccacccgtgg gcgacctgtt
     6361 tcctggacct gcattaaacg ctcatagacg ccgggcgcat gcactaacag gttcagcaat
     6421 tgcgccccag cccaaaagca aaacagcaca aacaacgcat aggcaatgtt gccaggcgtt
     6481 gaagaggcat tgcgatattt tccgctaaac agcgattttg acagggtgaa atcagccata
     6541 aaaacctccg gaagtcatct gcttctcttc tggtgtgata ccagcggctt atggcaagtc
     6601 tgacaggtca tttagattgt caatatcaga atcatggtaa attgatgttg ggaatattcc
     6661 cgaagcgtga atcttcgttg tgagtcacaa aatggcaacc ttaagttaaa tttattaaca
     6721 attacgaaaa tgttgtctac accacagacg gttgttacag aatatgcaag gatgttgttt
     6781 ttcgttaacg gagctgccat gaatctgcct gtaaaaatcc gccgtgactg gcactactat
     6841 gcgttcgcca ttggccttat attcattctt aatggcgtgg tggggttact gggatttgaa
     6901 gcaaaaggtt ggcagaccta tgccgtcggt ctggtgacgt gggtgattag tttctggctg
     6961 gcggggttga ttattcgtcg tcgcgatgaa gaaactgaaa acgcccaata agaatattat
     7021 cgggcgttaa aatattacat tgtggttttc aatgcgttat ccgcagcgtg acgctccagc
     7081 gccagctcaa tcaaacgtgt gatcagatcg gtgtaaccca gaccgctggc ttgccacagc
     7141 ttcggataca tactgatgtt ggtgaagcca ggcagtgtgt tgatctcgtt gatcaccact
     7201 tcgttctctg gggttaaaaa cacgtctaca cgcgccatgc ctgcgcatcc caacgtttga
     7261 taagcctgaa cggcaatcgc ccggatctta tcgttgattt ctggcgcaat ggctgccgga
     7321 actaccactt tcgcgccatc ttcgtcaatg tacttggtgt cgtaggcata gaagtcgctg
     7381 gtgagtacga tctcgccaca ggtgctggct tgcgggttgt cgttgcccag aactgcgcat
     7441 tcgatctcac gacctttgat cccttgctca acgatcactt tatgatcgaa ttcgaacgcc
     7501 agatcgacgg caattgcgta ctgttcttca ctggttactt tgctgacacc aacagaagag
     7561 ccctgattag ccggttttac aaacagtggt aacctcagtt tagactccac ttcggcaaaa
     7621 ctaatgttgt gacgattggc gcgcgtcagg gtaataaatg gcgcaatgtt cagcccggca
     7681 tcgcgcagca gacgtttggt aacatcttta tccatacagg ctgctgaagc cagaacatca
     7741 gaacctacaa acggtaaatt ggcgacccgc agcattccct gcaaggaacc atcttcgccc
     7801 agcgtaccgt ggacaatcgg gaaaatgaca tccaccgtcg gtaacggctg accgttttgc
     7861 gcgtcgataa gctgatgctc atgtttactt ggcacctgcg caaggctagt cgccgaaggg
     7921 cgcaacgcaa tatgggcagg atcgtctgca tttagcagat aattgctggc atcgctgacg
     7981 tgccattgcc cttgtttatc aatgcccagc agcacaacgt cgaagcgact tttatcaatg
     8041 gcatcgacaa tgttttttgc cgattgcaga gacacttcat gttccgctga tttaccacca
     8101 aaaacgattc ctacccgcag tttttccatc ttaaaaacct atcccgtcta acacaaagtg
     8161 catacattac cacgacaaaa cgggggattc gcggccttct gacagaatgt tgcaatcttc
     8221 tgctgacaaa gcgtgcaacg taatggtgaa gaaagtgcgt tattacaaag atgtgcgcga
     8281 gatcacaaaa atgatgaacg ggaagctaat ttattcctgg cttaaatggc catgcggtga
     8341 gttttttctc ttagttgtaa gtaaacgcag agaatatatt tcataacttt tgtttataat
     8401 aaaggttgat aattaaaggc ctgttctttg tgtggttaat tatttaaata tgagaaacgt
     8461 ttcgctggta atcaaacaaa gaacatttgc gcaaagtatt tcctttgtca taaaaataat
     8521 acttccagac actatgaagt tgtgaaacat aatgttaact tctccatacg ttggataagg
     8581 aaatacagac atgaaaaatc tcattgctga gttgttatta aagcttgccc aaaaagaaga
     8641 agagtcgaaa gaactgtgcg cgcaggtaga agctttggag attatcgtca ctgcaatgct
     8701 tcgcaatatg gcacaaaatg accaacagcg cttgattgaa caggtggagg gggctcttta
     8761 cgaggtaaag cctgatgcca gcattcctga cgacgatacg gagctgctgc gcaattatgt
     8821 aaagaagtta ttgaagcatc ctcgtcagta aaaagttaat cttttcaaca gctgtcataa
     8881 agttgtcacg gccgagactt atagtcgctt tgtttttatt ttttaatgta tttgtacatg
     8941 gagaaaataa agtgaaacaa agcactattg cactggcact cttaccgtta ctgtttaccc
     9001 ctgtgacaaa agcccggaca ccagaaatgc ctgttctgga aaaccgggct gctcagggcg
     9061 atattactgc acccggcggt gctcgccgtt taacgggtga tcagactgcc gctctgcgtg
     9121 attctcttag cgataaacct gcaaaaaata ttattttgct gattggcgat gggatggggg
     9181 attcggaaat tacagccgca cgtaattatg ccgaaggtgc gggcggcttt tttaaaggta
     9241 tcgatgcctt accgcttacc gggcaataca ctcactatgc gctgaataaa aaaaccggca
     9301 aaccggacta cgtcaccgat tcggctgcat cagcaaccgc ctggtcaacc ggtgtcaaaa
     9361 cctataacgg cgcgctgggc gtcgatattc acgaaaaaga tcacccaacg attctggaaa
     9421 tggcaaaagc cgcaggtctg gcgaccggta acgtttctac cgcagagttg caggatgcca
     9481 cgcccgctgc gctggtggca catgtgactt cgcgcaaatg ctacggtccg agcgcgacca
     9541 gtgaaaaatg tccgggtaac gctctggaaa aaggcggaaa aggatcgatt accgaacagc
     9601 tgcttaatgc tcgtgccgac gttacgcttg gcggtggcgc aaaaaccttt gctgaaacgg
     9661 caaccgccgg tgaatggcag ggaaaaacgc tgcgtgaaca ggcacaggcg cgtggttatc
     9721 agttggtcag cgatgccgcc tcgctaaatg cggtgacgga agcgaatcag caaaaacctc
     9781 tgcttggact gtttgctgac ggcaatatgc cagtgcgctg gctaggaccg aaagcaacgt
     9841 accacggcaa tatcgataag cccgcagtca cctgtacgcc taatccgcaa cgtaatgaca
     9901 gtgtaccaac cctggcgcag atgaccgaca aagccattga attgttgagt aaaaatgaga
     9961 aaggattttt cctgcaagtt gaaggtgcgt caatcgataa acaggatcac gctgcgaatc
    10021 cttgtgggca aattggcgag acggtcgatc tcgatgaagc cgtacaacgg gcgctggaat
    10081 tcgctaaaaa ggatggcaac acgctggtca tagtcaccgc tgatcacgcc cacgccagcc
    10141 agattgtcgc gccggatacc aaagctccgg gcctcaccca ggcgctaaat accaaagatg
    10201 gcgcagtgat ggtgatgagc tacgggaact ccgaagagga ttcacaagaa cataccggca
    10261 gtcagttgcg tattgcggcg tatggcccgc atgccgccaa tgtcgttgga ctgaccgacc
    10321 agaccgatct cttctacacc atgaaagccg ccctggggct gaaataaaac cgcgcccggc
    10381 agtgaatttt cgctgccggg tggttttttt gctgttagca accagactta atggcaaatc
    10441 tcgggcgcat acgctcatgg ttaaaacatg aagagggatg gtgctatgaa aattacatta
    10501 ctggttacct tgtttttcgg tctggttttt ttaaccacag tcggcgctgc cgagagaacg
    10561 ttaaccccac aacaacagcg tatgacctcc tgtaatcagc aggcgacggc gcaggcgttg
    10621 aaaggggatg ctcgtaagac ctacatgagt gattgcctga agaacagcaa gtctgcgcct
    10681 ggtgaaaaaa gtttgacgcc acagcagcaa aagatgcgcg aatgtaataa tcaagcaacg
    10741 caacaatctc tgaaaggtga tgatcgtaat aagtttatga gtgcctgcct caagaaagcc
    10801 gcctgatacc tgatagtgct aacgggtgag ctacgaaagt ggctcacccg aaatttcata
    10861 cttctgcctt tagccccgtc tctataattt gggaaaattg tttctgaatg ttcccaaaaa
    10921 taatgaatga tgaaaacttt ttcaaaaaag cggcggcgca tggggaggaa cctcctttaa
    10981 ctcctcaaaa cgaacatcag cggtccgggc tgcgcttcgc ccgtcgcgtc agactacccc
    11041 gtgcggttgg cctggctggc atgttcttac cgattgcttc aacgctggtt tcgcacccgc
    11101 cgccgggctg gtggtggcta gtgttggtcg gctgggcgtt cgtctggccg catttagcct
    11161 ggcagatagc gagcagggcc gtcgatccgc ttagccggga aatttacaac ttaaaaaccg
    11221 atgcagtatt agcgggaatg tgggtaggcg taatgggcgt aaacgtgctg ccttccaccg
    11281 ctatgttgat gattatgtgt ctgaatttga tgggggcagg cggcccccgt ctgtttgtcg
    11341 cgggtctggt gttgatggtg gtttcctgcc ttgtcaccct cgagcttacg ggcattaccg
    11401 tgtcgttcaa tagtgcgccg ctggaatggt ggctctccct tcccattatt gtcgtttatc
    11461 ctctgctgtt tggctgggtc agctaccaga cggcaaccaa actggcggaa cataaacgca
    11521 ggttgcaggt catgagtacc cgcgacggca tgacgggcgt gtataaccga cgtcattggg
    11581 aaactatgtt acgcaatgaa tttgataact gtcggcggca taatcgcgat gcaacgttac
    11641 tgattatcga tatcgaccat ttcaagagca tcaacgatac ctggggtcat gatgtgggcg
    11701 atgaagcgat tgtggcgctt acccgacagt tacaaattac cctgcgcggt agcgatgtga
    11761 ttggtcggtt tggcggcgat gagtttgcag taatcatgtc cggtacgcca gctgagagcg
    11821 ccattaccgc catgttacgg gtgcgtgaag ggctaaacac gttacgcttg ccgaatacgc
    11881 cacaggtaac tttacggatt agtgtggggg ttgcgccgct gaacccacaa atgagtcact
    11941 atcgtgagtg gttgaaatcg gcagatttgg cgctttacaa agcaaagaaa gccggacgta
    12001 accgcaccga agtggcggcc tgacgtcagg cgaaagtcat caggatttgc tgagtttttc
    12061 tgatttttcc atacacttcg tcatcgcttc gatcactgcg gcacggaagc ctttctcttc
    12121 cagtacgcgt accgcttcaa tggtggtgcc tcccggtgag cagaccatat ctttcagtgc
    12181 ccccggatgt tctcccgttt ccagcaccat ttttgcggaa cccattaccg cctgagcggc
    12241 aaatttatac gcctgggcgc gtggcatccc gcccagcacg gcggcgtcgg ccatcgcttc
    12301 gataaacata aatacgtagg ctggcgaaga accgctcaca ccgactaccg ggtggatcat
    12361 cggctcagca attacttccg cttcgccaaa gcagcggaaa atattcagca catcagcggt
    12421 atcttctggg gttaccagcg cgtttggcgt tacggaggtc atcccggcat taaccagcgc
    12481 gggagtgttc ggcatggcgc ggataatttt ccggtcatgg cccagcgcgc gggcaagctg
    12541 gtcgagcgtg acacctgcag caatagaaac gaccagagag tctttattca ggctggaggt
    12601 gatttcgcta agcactttaa tcatgatgcc aggtttaacg gcagcaaaaa tgatgtcggc
    12661 gatttgcgcc acttcttgcg ccgattctgc ggcgttgatg ccgaactggt catgcagggc
    12721 ggcgacttta ttcggggagg gggtgtatac ccagatttgc cctggaagca cctgaccgct
    12781 ggcaatcaga ccgccgagaa tggcttttcc catattgccg cagccaataa aaccgatttt
    12841 cttttccatt gcctcactcc tgccgtgaaa ttcattgttt tgataatcgc tggcagaagc
    12901 ataaacagaa ctatgccgga aggcaaaagc gcgacacaat agaggattac ccaacaaagg
    12961 atgactttat gacaatttgg gtggatgctg acgcgtgtcc caatgtaatt aaagagattt
    13021 tgtatcgcgc ggcggaacgt atgcagatgc cgctggtact ggtagcaaac cagagtttac
    13081 gcgtgccgcc atcgcgattt attcgtacgc tgcgcgtcgc ggcaggtttc gacgttgccg
    13141 ataacgaaat tgtccggcag tgtgaagcgg gcgatttggt gatcaccgca gatatacctt
    13201 tggctgctga agccatcgag aaaggcgcag cggcgcttaa tccgcgcggc gaacgttaca
    13261 cgccagcgac cattcgtgag cgcctgacga tgcgcgattt tatggatacc ttacgtgcca
    13321 gtgggatcca gaccggcgga ccagatagcc tttcacaacg tgaccgccag gcctttgccg
    13381 cggagctgga gaagtggtgg ctggaagtgc aacgtagtcg tggctaaatg taatttatta
    13441 tttacacttc attcttgaat atttattggt atagtaaggg atgtattgag attttcactt
    13501 taagtggaat tttttcttta caatcgaaat tgtactagtt tgatggaatg atcgctattc
    13561 tcatgacacc ggctttcgcc gcattgcgac ctattgggga aaacccacga tgacacaacc
    13621 tctttttctg atcgggcctc ggggctgtgg taaaacaacg gtcggaatgg cccttgccga
    13681 ttcgcttaac cgtcggtttg tcgataccga tcagtggttg caatcacagc tcaatatgac
    13741 ggtcgcggag atcgtcgaaa gggaagagtg ggcgggattt cgcgccagag aaacggcggc
    13801 gctggaagcg gtaactgcgc catccacagt tatcgctaca ggcggcggca ttattctgac
    13861 ggaatttaat cgtcacttca tgcaaaataa cgggatcgtg gtttatttgt gtgcgccagt
    13921 atcagtcctg gttaaccgac tgcaagctgc accggaagaa gatttacggc caaccttaac
    13981 gggaaaaccg ctgagcgaag aagttcagga agtgctggaa gaacgcgatg cgctatatcg
    14041 cgaagttgcg catattatca tcgacgcaac aaacgaaccc agccaggtga tttctgaaat
    14101 tcgtagcgcc ctggcacaga cgatcaattg ttgattttag agcgcctata cttaacgttc
    14161 attccgtgaa ataaggaaga acgatgccaa cgaaaccgcc ttatcctcgt gaagcatata
    14221 tagtgacgat tgaaaaagga aagccaggac agacggtaac ctggtaccaa ctcagagccg
    14281 atcatcctaa accagactcg ttgatcagtg aacatccgac cgctcaggaa gcgatggatg
    14341 cgaaaaaacg ctatgaggac cctgacaaag agtgaccgca tcagactgct cggaagggat
    14401 tctgagtgcc actacaaggg atctgcgtca catttttcat aattcatgtt tttctaataa
    14461 ttagaatatt aaacaataac aatccattac tggaatcatt tggaatcttt acattatgcc
    14521 gtgcacgtct gctgctacgc tttttgtcat ttgtagcaca agtaagtgtc agcagtggtg
    14581 cttcacactt gcccggtaat taacgacgaa agaaaagtaa ggtggatgaa caatgagtgc
    14641 gtcgttggcg atcctgacca tcggcattgt acctatgcag gaggttttgc cgctcctgac
    14701 ggaatacatt gacgaagata atatttccca tcatagcctg ctggggaagt taagtcgtga
    14761 agaagtgatg gcggagtacg cgccagaagc aggcgaagac accattctca cattattaaa
    14821 tgacaaccag ctggcccatg tttcgcgtcg taaagtggag cgagacctgc aaggtgtggt
    14881 tgaagtgctc gataatcagg gttatgacgt cattttatta atgagtacag caaacattag
    14941 tagtatgact gcgcgtaata cgatctttct tgagccgtcg cgaatattgc ctccactggt
    15001 ttcctctatt gttgaagatc atcaggtggg ggttatcgtt ccggttgagg agatgctgcc
    15061 cgttcaggcg caaaaatggc aaattttgca gaaatcgccg gtattttcat tgggtaaccc
    15121 cattcatgat tcagaacaaa aaatcattga tgcagggaaa gaattactgg caaaaggggc
    15181 tgatgtcatc atgctggatt gtttaggatt tcaccaacgt catcgcgatt tactgcaaaa
    15241 acagctcgat gttcctgtct tgctgtctaa cgtattgatt gcacggctgg ctgcggaatt
    15301 actggtgtaa ttttgcgtga cagccagcgc ctctggcccc tatagtgaag tagatgttca
    15361 actaccagac agggccagtt tatgcttcaa agtaatgagt acttttccgg caaagtgaaa
    15421 tcaatcggct tttccagcag cagcactggt cgcgccagcg tgggtgttat ggttgaaggc
    15481 gaatacacct tcagcaccgc tgagccggaa gagatgacgg taatcagtgg cgcgctgaat
    15541 gtgttactgc ctgatgcgac cgactggcag gtgtatgaag ccggttcggt gtttaatgtt
    15601 cccggtcaca gtgagtttca tctgcaagtt gccgaaccca cctcttatct gtgccgctat
    15661 ctgtaattcc tcgccttccc cttgaacggg agggcatttt tctgaaatat cctctcttta
    15721 acccataata atatttcctt tgctgcgatt ttttcaattt ccgatatatt cataattcat
    15781 caaggttgat ataaatatca gtgaagatct ccagatattg ttgcggaact ggctacgata
    15841 aaagataaat cagatgatga atggtggcgt gcattgctgc aaagtcgcaa tgatcatttg
    15901 cgccaaacgg cattacgcaa cgcacatacc ccggcgtcgt tgttaacgac attgaccgag
    15961 tctcggcatc ggtcgctagc tatgaataat ccacagctcg ctgccgatgt gaaaacgacg
    16021 tggttaaaag aggatccatc attactctta tttgtcgaac aacccgatct ttcgctgtta
    16081 cgggatttag tgaaaaccgg ggcaatgcgg aaaattcgca gtgaagcgcg tcactggctt
    16141 gaggaaaaac aatgacacaa cgtccatgga gcaagttaca gcataaaacg cataatattg
    16201 ctgcgctaaa aattattgtc cgccgtagcg aataattata tccctggtgt ggcttcgtac
    16261 gccggataag acgcggcagg cgtcgcatcc ggcattaaag gaaaatcagc aattaacgtt
    16321 gtgcttcgcc acctaatcct tcaatcaggt tttgaattaa cgctgccagt tcaccagtca
    16381 tcaggatgaa atcggcatca aaacgctggg cgaaatcttc acggtcgata tcttcgtttt
    16441 gatcgcgcag ctcgtcgcaa aacttcagac gcttgagcga accatcgtcg cacatcacaa
    16501 actgaatgcg ctgctgccag tcgagcgcca gtttagtcac cacttttccg gcttcgatgt
    16561 gattggtgat ctcttcgctg gtcagatctt gtttcttcgc gcggatcacg ccgccatctt
    16621 ccagcaacga tttcagctcg gcttcatcaa gcagctggaa gccctgtgcc gcgctaccgg
    16681 agcgaaccca ttcggtcagc gtcagttcaa tcgggttttc catgctcaat ggtaccacag
    16741 gtaacgaccc caggctttta cgcagcagtg ccagcgtatc ttccgctttt ttggcactgg
    16801 cgcagtccac cataatcaaa ccgttaaccg tgtcgatcca catcattgtc tggctaaaac
    16861 ggctgaaagc acgcggcaac agagagtgca gcacttcgtc tttcagcgaa tctttttcgg
    16921 ttttcttcag cttacgcgcc tgttccgctt ccagtttggc gattttcgct tccagcgcct
    16981 gcttaatcac cggagacggg aggatttttt cttctttgcg cgcgcagata acaatttgac
    17041 cattggcaac gtgcgttaac gcatcgctgt gcgatcccat cggaggaacc cagcccatct
    17101 tcgccatgtc ctggctgccg catggggtaa atgccatcga ggctagctgt ttttccatct
    17161 cttctgcacg cagcgaaatc tcgcggctaa gacggtaaac cattaaattt ttgaaccaca
    17221 gcatgataat ttccacggcc ttgttgttaa atttagcggg catgataacg aattgtcggc
    17281 ggccttgcat tgccaatccg gttgtccgtc tctacgctat tgatattgaa aaaaataagg
    17341 agagtaccgt gcgtataggt atcgatttag gcggcaccaa aactgaagtg attgcactgg
    17401 gcgatgcagg ggagcagttg taccgccatc gtctgcccac gccgcgtgat gattaccggc
    17461 agactattga aacgatcgcc acgttggttg atatggcgga gcaggcgacg gggcagcgcg
    17521 gaacggtagg tatgggcatt cctggctcaa tttcgcctta caccggtgtg gtgaagaatg
    17581 ccaattcaac ctggctcaac ggtcagccat tcgataaaga cttaagcgcg aggttgcagc
    17641 gggaagtgcg gctggcaaat gacgctaact gtctggcggt ttcagaagca gtagatggcg
    17701 cggcagcggg agcgcagacg gtatttgccg tgattatcgg cacgggatgc ggcgcgggcg
    17761 tggcattcaa tgggcgggcg catatcagcg gcaatggcac ggcaggtgag tggggacaca
    17821 atccgctacc gtggatggac gaagacgaac tgcgttatcg cgaggaagtc ccttgttatt
    17881 gcggtaaaca aggttgtatt gaaaccttta tttcgggcac gggattcgcg atggattatc
    17941 gtcgtttgag cggacatgcg ctgaaaggca gtgaaattat ccgcctggtt gaagaaagcg
    18001 atccggtagc ggaactggca ttgcgtcgct acgagctgcg gctggcaaaa tcgctggcac
    18061 atgtcgtgaa tattctcgat ccggatgtga ttgtcctggg gggggggatg agcaatgtag
    18121 accgtttata tcaaacggtt ccgcagttga ttaaacaatt tgtcttcggc ggcgaatgtg
    18181 aaacgccggt gcgtaaggcg aagcacggtg attccagcgg cgtacgcggc gctgcgtggt
    18241 tatggccaca agagtaaaaa acgtaggcaa ttggcgcatc atgcctgatg cgacgcttgc
    18301 cgcgtcttat caggcctgca aaatgtgcca gaaccgcgta gggcggataa ggcgttcacg
    18361 ccgcatccgg caataagtaa tgaacaccga gactataacc taccccagtg gtttcgccag
    18421 caccggagta tccgccgctt gctggcgctt atagcgacca tacagcagca acgacgacat
    18481 cgcagcaaac gaaagcaggg cggcaggcag cgccacgtaa ttatatgcca gccccagcgt
    18541 cagcatcata ccaccgcaat atgcgccgac ggcgctaccg aggttaaacg ctatttgccc
    18601 acctgcggca cctaataact ctccgccttt ggcgttttgt agtaacaata tttgtagcgg
    18661 tgctgaaagg gcaaataatc ccgcgcaaca aataaaagca aaaataagcg acgttgtttt
    18721 catgccgccg cagaaaaaga acatcagcag tgccagtaca attataaagt cagtcactgc
    18781 tgcaatgcgc agtggtgaat aacgtcctga aatcctgcca cttagcatat ttcccagcac
    18841 catccctagc ccaactaaca tcataataaa ggtcatcgcc gtttccgaaa aaccggaaat
    18901 aaacatcatg tatggcttta cgtagctgaa ccaggcaaac acacctgcgt tgccaaacat
    18961 ggtggcggcg aaaattaacc acggggccgg gctgcgcaaa aagtgaaatt gttcgcgcag
    19021 ctttcctttc gcctcgtcgc gaatatccgg cacccaaaaa tagaccgatg ccatcaccgc
    19081 aatattaaaa acagcgatca ataaaaaggt gtaacgccag ctaaattcct gacttaaata
    19141 cgttcccagc ggaatgccca gcaaattggc gactgtcatc ccggaaacca tccccgccac
    19201 ggcggcggtg acttttccgg gtttgataat ttttgataac acgatcgcgc cgacgccaaa
    19261 aaatgcgcca tgcggaaagc cggataccag ccgaccaatg gcgagcatca ggtaagacga
    19321 agagagcgtg aacatggcgt tgccaatgac gcacaacgcc accagaaaca acaagatatg
    19381 tttgagtgag tagcggctgg aaaagagtgc gatgattggc gcaccgacca ccactcccag
    19441 tgcataatac gagatcatat gcccggcagc aggaatcgaa attcctacgt tatgcgccag
    19501 ctccgtgagc acgcccataa tgccaaattc ggccatcccc aaaccaaacg tgcccagagc
    19561 caaagataaa atgacttttt tcataccacc atcacaacca ccagaacggc taccaatcac
    19621 cgtagccaat aaagtgatag cttgcaggat aacgaccagc aaggccaaga gataattcgc
    19681 ccctctgtat tcattatcct gctgaatagt tatttcactg caaacgtact ttccagtttg
    19741 ctgtagccca ggccgttgat ctttttcact ttgatctgca ccggaatacg ctctttcatc
    19801 gcttctacgt ggctaatcac accgatggtt ttgccactgg cgttcagggc atccagcgca
    19861 tcaagggcgg tatccagcgt ttcgctatcc agcgtgccaa aaccttcatc aaggaacagc
    19921 gagtcaatac gtgttttatg gctgaccaga tccgaaagcg ccagcgccag actaacgagg
    19981 aaactttcgc cgccggaaag ggtacgggta tcgcgtaccg catctgcctg ccaggtatca
    20041 acaacctcga cttccagcgc ctcgctggct ttgcgctgta acagatagcg cccgtgcagc
    20101 cgggtaagtt gctgattagc gagatggact aaattatcca gcgtcagccc ctgggcaaac
    20161 ttgcggaatt tatcgccctc tttggaacct attagcgaat tcagatatcc ccagtcctca
    20221 acctgctgcg tcatttgagc aatttgctgc attaaggttt gttgttgctg acggttatct
    20281 gcatcctgct tcagctgctg gcgaatctcg ccttgactcg tggtgttttc acgcaacttt
    20341 tggtgagttt gcgctaactc ttgctgaatc tgctccaccg tcacagtgag agccaacccg
    20401 tcaggtcggt gttgttgatg ctgtgccagc gtttctgctg tctgagtgac cagcgtttgc
    20461 gcctgacggc gctggttttc cagattctgc ttgagctgtt ccagctgcgt tagtgtttgt
    20521 tcatccatta gcgccgcaag gaacgcctgc tgatcgtcaa agacgctggc ctgtagcgcg
    20581 gtgtcaaact gcgcctgggc tttttgcaga ctttgcgccg ccagaacatc ctgttgctgt
    20641 aacgtctgct gctggctgtg taatgcgaga cattgttcat gaacctgccg ccagttatcc
    20701 agcgccacag tttcttcgct gtgcgggaga tcatcacttt gcggcaacgt ttccagaatc
    20761 ggcgtcagct gctgaatacg gttttgcagc gtggttaatt cgttctggcg ttgctgccag
    20821 ctctgcgctt cttgctgacg tgtcgccaac cagctctctt cttcatcttc ctgtggcaat
    20881 gtcagtgcat aacccgccaa tgccgttaag agctgctgct ggcgttgttc aatttgctgt
    20941 tgatactgga taatttgctg attatgcgcg gcaatctgcc cttgtaattc atgtcgttgg
    21001 ctgagtaacc gcagctggcg tttgtgctca tcttgtgcat ccagccacgg ttgaatatcg
    21061 tcctgtggct gcaaggtgat attgaggctg gccgtgacgg cttgccattg ttgagtaagt
    21121 gcttgctcat cttgtcggag gctttgcgct tcgttttcat cgcgctgaag ctgcttcgtt
    21181 aatgcatcca gttgcccgcg cagcgccgca ccttcttcgc cgagcttttt aacttcgttt
    21241 tccagcgcca gtaatcgaga ctgattaaca ccaggctcca gcgcctgata cgccttgacc
    21301 gccgggtggc tggtggaacc acaaagtgga caaggctgac ccgcctgtaa ctgtgcacgc
    21361 tgggcttcca gcgttttgat gcgcgcttcc tgctcgcaaa tggttttcac atcggcaagt
    21421 tgctgcgtct tttctttata acgctggcgc atttcgttaa gtgcgacatt acgttgcgtc
    21481 tgttccagag atccattctg gatagtgacc attaactgcg ccagacgttt ttgttgggga
    21541 acaatctgcc catgcagcgc gaccaggcgc tgacgcagtg ggcgttgctc agcatgttgc
    21601 gccagggcgg tagcaacttc atcggcggtt aacgtcaacg tgatcgccgc aagcgcatta
    21661 agtttttgct cagcatgggt taactgttgc tgccattgcc gcagatgctc gcgatcgctg
    21721 gtttgttggg agaactgcgc acgccaaccc gccagttcgt tgttccactg acggaagcgg
    21781 tcgtgttcct gtaaccaggt attcaggctt tgttgctgct gctgtaattc tgctgactgc
    21841 ttcgccgcgt ggtggcgaat gctcgcgcga agcgccattg tgctctgtaa gcgagtattt
    21901 acttcttcaa tctgctggcg agtatgcgcc agcgccgcgc tgtgttctgc gatgcgttcc
    21961 cagtgcggac gaagatttcg tgccggttgt gccagactga gcgccgccag ttgaggttgc
    22021 gctttttctt cttcggctaa cgcctgttgc aaggcctgct gacggcggct ggcttcttgc
    22081 tgcaattcgt ccagacgcgt taaccagttt agtgattgtt gttcttgctg ctgcgcggta
    22141 agtaactgtt tttcttcgtc agtaagtacc tgcaaactcg ctgtcagcga ttgcacttgt
    22201 tccggcgtga gcaacgcgac gccgctggcc tgcgcttgca gcttctccag ctctgtgcgg
    22261 gccgatttgt gctgctcaaa aaccatcgcc gagatttgcc cgtagatttc agtgccggtt
    22321 aactcctcga gcaattccgc gcgttctttg ggtttggcat tcaggaaggc agcaaattgc
    22381 ccctgcgaaa gcagcatcga acgggtgaag cgcccgtaat ccagcccggt taacgtcgct
    22441 gtcagttcca gcttatcttt cactttgtcg gcgagaattt tgccgtcggc gcagcgcgcc
    22501 agctctacgc gtggcacctg caaattaccg tcgggttggt tacgcgcccg attctggctc
    22561 cagaatgcac ggtacgcttc acctttcact tcaaactcca cctccgccag acattcggcg
    22621 gtatcgcggg tcatgagatc attttgcgat tgtgaaacgt tagagagacg cggagtttcg
    22681 tgatacagcg ccagacaaat ggcgtccagc agggtggttt tccccgcacc ggttgggccg
    22741 gtaatggcaa acagcccgtt gctggcgaac ggctcgcggg tgaaatcaat cttccattcg
    22801 ccttttaatg agttcaggtt tttcaggcgc aggctgagaa ttttcatgct tcgtgttctc
    22861 cggcgagggt atgcaacgtc gtggtgaaaa gatgctgcag acgttgctgc tgcgattcat
    22921 ccagttcttc cagtgccaga cggcgattga acacctcttc gacgctgagt tcgctgaggg
    22981 tttcacgctg ttggctggct aacacacgct cgcgctgttc acgactccga cgtaccagca
    23041 atacttcgac aggcaatgat tcggctaatg cctggatttt gcgctgaata tcatgcagat
    23101 actcatcagt agtgatttcg atatccagcc agacaggtgg ctcctgcgat acatcgcgcc
    23161 actgttccag ctgtgcggta atcgacgcca gatcgccttt cagcaccgcc atgggttgag
    23221 ttaccggtac gttcaggttt tccacgctct ctaatttgcc gtttgaaaat gtcaccagat
    23281 ggacatattt actcttaccg cattcatcaa aactcagtgg aatgggggaa ccgcaatagc
    23341 gaacatgttc catgccgcca ataatctgtg cgcggtgaat atgcccgagc gcgatgtagt
    23401 cggctggtgg aaagttttgt gccggaaacg cgtccagcgt gccaatataa atgtcacgca
    23461 cggcgtcact tttactggca cccacggtcg ttaaatgtcc cgtggcgatg atgggcagag
    23521 gctgatcgcc gcgcagtttg caggcatcgg catagtgttg ttggtaataa tcggtaatcg
    23581 ctgccagtaa atgctgctgt ttttcaatac cgttaagccc cgcctggctg gaaataatgt
    23641 cacgcgggcg taaaaacgga atggggcaca gcactgcgcc tggcgtcccg tcgcgacgag
    23701 gcaagatttg cggcgcatgt ccggcgctgg cgaccacggt tgtattgagg aacgccatga
    23761 tatcgcgcga ttcattcagc gtggcgaccg agtcatggtt tcctgccagt accaccagat
    23821 gacagccagt ttgctgtaaa ttgacaacaa aacggttgta taacgtgcgg gcgtaactgg
    23881 gcggcgagcc ggtatcgaaa acatcaccag caacaataat cgcatccacc tgatgggttt
    23941 gtgctgtctc cagcagccag tcaagaaaag cctgatgttc agcttcgcgg cttttactgt
    24001 agaagttctg gccgagatgc cagtctgagg tgtgaaggat gcgcataacg gttccctggc
    24061 gaaaaagcat ggtcgcgatt atacccaaac agatgtgcca tttgcttttt tctgcgccac
    24121 ggaaatcaat aacctgaaga tatgtgcgac gagcttttca taaatctgtc ataaatctga
    24181 cgcataatga cgtcgcatta atgatcgcaa cctatttatt acaacagggc aaatcatggc
    24241 gagacgtatt ctggtcgtag aagatgaagc tccaattcgc gaaatggtct gcttcgtgct
    24301 cgaacaaaat ggctttcagc cggtcgaagc ggaagattat gacagtgctg tgaatcaact
    24361 gaatgaaccc tggccggatt taattctcct tgactggatg ttacctggcg gctccggtat
    24421 ccagttcatc aaacacctca agcgcgagtc gatgacccgg gatattccag tggtgatgtt
    24481 gaccgccaga ggggaagaag aagatcgcgt gcgcggcctt gaaaccggcg cggatgacta
    24541 tatcaccaag ccgttttcgc cgaaggagct ggtggcgcga atcaaagcgg taatgcgccg
    24601 tatttcgcca atggcggtgg aagaggtgat tgagatgcag ggattaagtc tcgacccgac
    24661 atctcaccga gtgatggcgg gcgaagagcc gctggagatg gggccgacag aatttaaact
    24721 gctgcacttt tttatgacgc atcctgagcg cgtgtacagc cgcgagcagc tgttaaacca
    24781 cgtctgggga actaacgtgt atgtggaaga ccgcacggtc gatgtccaca ttcgtcgcct
    24841 gcgtaaagca ctggagcccg gcgggcatga ccgcatggtg cagaccgtgc gcggtacagg
    24901 atatcgtttt tcaacccgct tttaacgcct tgctcatcgg acgcagagca gggcttatga
    24961 tttcttaact ggagtatctt acgtgctgga acggctgtcg tggaaaaggc tggtgctgga
    25021 gctgctactt tgttgcctcc cggctttcat cctgggtgca ttttttggtt acctgccctg
    25081 gtttttgctg gcatcggtaa caggactgct tatctggcat ttctggaatt tattgcgcct
    25141 ttcatggtgg ttgtgggtgg atcgcagtat gaccccgcca ccggggcgtg gtagctggga
    25201 accgctacta tacggcttac accagatgca gctgcgaaat aaaaaacgcc gccgtgaact
    25261 gggcaatctg attaaacgct ttcgtagcgg cgcggagtcg ctgcctgacg cggtggtgct
    25321 gaccacggaa gagggcggta ttttctggtg taacggtctg gcgcaacaaa ttcttggttt
    25381 gcgctggccg gaagataacg ggcagaacat ccttaaccta ctgcgttacc cggagtttac
    25441 gcaatatctg aaaacgcgtg atttttctcg cccgctcaat ctggtgctca acaccgggcg
    25501 gcatctggaa attcgcgtca tgccttatac ccacaaacag ttgctgatgg tggcgcgtga
    25561 tgtcacgcaa atgcatcaac tggaaggggc gcggcgtaac ttttttgcca acgtgagcca
    25621 tgagttacgt acgccattga ccgtgttaca gggttacctg gagatgatgg atgagcagcc
    25681 gctggaaggc gcggtacgcg aaaaagcgtt gcacaccatg cgcgagcaga cccagcggat
    25741 ggaagggctg gtgaagcagt tgctaacgct gtcgaaaatt gaagccgcgc cgacgcaatt
    25801 gctcaatgaa aaggttgatg tgccgatgat gctgcgcgtt gttgagcgcg aggctcagac
    25861 tctgagtcag aaaaaacaga catttacctt tgagatagat aacggcctca aagtgtctgg
    25921 caacgaagat cagctacgca gtgcgatttc gaacctggtc tataacgccg tgaatcatac
    25981 tccagaaggc acgcatatca ccgtacgctg gcagcgagtg ccgcacggtg ccgaatttag
    26041 cgttgaagat aacggaccgg gcattgcacc ggagcatatt ccgcgcctga ccgagcgttt
    26101 ttatcgcgtt gataaagcgc gttcccggca aaccggcggt agcggattag ggttagcgat
    26161 cgtgaaacat gctgtgaatc atcacgaaag tcgcctgaat attgagagta cagtaggaaa
    26221 aggaacacgt ttcagttttg ttatcccgga acgtttaatt gccaaaaaca gcgattaatc
    26281 cgcctttgtc atcttttatt gccataagcc agtcgatgct ggcttatttt ctttgcagtc
    26341 aaaatacggg cgttagattt tacaacggtt ggtgattttt tgttcgcatg attagccatg
    26401 tctttttcac ggaaatagtg ttttatactg gttggtgatt tcttatcgct atatacctct
    26461 ggtttttaga tccctccttg ctttaaaacg ttataagcgt ttaaattgcg cttcaggtgc
    26521 tgtcatactg actgcattaa cgcggtaaat cgaaaaacta ttcttcgccg cgccaggttg
    26581 ggagtatttc ccgctaaaat tgtttaaata taccgctgta tcatccccag ggattggcac
    26641 aaaaatttaa cgttacaaca ccacatccac aggcagtatg atttatgacc catcaattaa
    26701 gatcgcgcga tatcatcgct ctgggcttta tgacatttgc gttgttcgtc ggcgcaggta
    26761 acattatttt ccctccaatg gtcggcttgc aggcaggcga acacgtctgg actgcggcat
    26821 tcggcttcct cattactgcc gttggcctgc cggtattaac ggtagtggcg ctggcaaaag
    26881 ttggcggcgg tgttgacagc ctcagcacgc caatcggtaa agtcgctggc gtgctgctgg
    26941 caacggtttg ttacttggcg gtggggccgc ttttcgctac gccgcgtaca gctaccgttt
    27001 cctttgaagt ggggattgcg ccgctgacgg gtgattccgc gctgccgctg tttatctaca
    27061 gcttggtcta tttcgctatc gttattctgg tttcgctcta tccgggcaag ctgctggata
    27121 ccgtgggcaa cttccttgcg ccgctgaaaa ttatcgcgct ggtcatcctg tctgttgccg
    27181 ctattgtctg gccggcgggt tctatcagca cggcgactga ggcttatcaa aacgctgcgt
    27241 tttctaacgg cttcgttaac ggctatctga ccatggatac gctgggcgca atggtgtttg
    27301 gtatcgttat tgttaacgcg gcgcgttctc gtggcgttac cgaagcgcgt ctgctgaccc
    27361 gttataccgt ctgggctggc ctgatggcgg gtgttggtct gactctgctg tacctggcgc
    27421 tgttccgtct gggttcagac agcgcgtcgc tggtcgatca gtctgcaaac ggcgctgcta
    27481 ttctgcatgc ttacgttcag cacacatttg gcggcggcgg tagcttcctg ctggcggcgt
    27541 taatcttcat cgcctgcctg gtaacggcag ttggcctgac ctgtgcttgt gcagaattct
    27601 ttgcccagta cgtaccgctc tcttatcgta cgctggtgtt tatcctcggc ggcttctcga
    27661 tggtggtgtc taacctcggc ttaagccagc tgatccagat ctccgtaccg gtgctgaccg
    27721 ctatttatcc gccgtgtatc gcactggttg tattaagttt tacacgctca tggtggcata
    27781 attcgtcccg cgtgattgcc ccgccgatgt ttatcagcct gctttttggt attctcgacg
    27841 ggatcaaagc atctgcattc agcgatatct taccgtcctg ggcgcagcgt ttaccgctgg
    27901 ccgaacaagg cctggcgtgg ttaatgccaa cagtggtgat ggtggttctg gccattatct
    27961 gggatcgcgc ggcaggtcgt caggtgacct ccagcgctca ctaaatcact gaacatttgt
    28021 tttaaccacg gggctgcgat gccccgtggt tttttattgt gttgatgggt taggaattga
    28081 tggaaagtaa gaacaagcta aagcgtgggc taagtacccg ccacatacgc tttatggcac
    28141 tgggttcagc aattggcacc gggctgtttt acggttcggc agacgccatc aaaatggccg
    28201 gtccgagcgt gttgttggcc tatattatcg gtggtatcgc ggcgtatatc attatgcgtg
    28261 cgctggggga aatgtcggta cataacccgg ccgccagctc tttctcgcgt tatgcgcagg
    28321 aaaacctcgg cccgctggca ggttacatta ccggctggac ctactgcttt gaaatcctaa
    28381 ttgtcgccat cgccgatgtg accgcttttg gtatctatat gggtgtctgg ttcccgacgg
    28441 tgccgcactg gatttgggta ctgagcgtgg tgctgatcat ttgcgccgta aacctgatga
    28501 gcgtgaaggt attcggtgag ctggaattct ggttctcgtt ctttaaagtc gccaccatca
    28561 tcatcatgat tgtcgccggt ttcggcatca tcatctgggg gattggcaac ggcgggcaac
    28621 cgaccggtat tcataacctg tggagcaacg gcggcttctt cagtaacggc tggcttggca
    28681 tggtaatgtc gttgcaaatg gtgatgtttg cttacggtgg gatcgaaatt atcgggatta
    28741 ccgccggtga agcgaaagat cctgagaaat cgattccgcg tgcgattaac tccgtgccga
    28801 tgcgtattct ggtgttctac gtcggtacgc tgttcgtcat tatgtctatc tacccgtgga
    28861 atcaggttgg cactgccggt agcccgttcg tgctgacgtt ccagcatatg ggcattacct
    28921 ttgccgccag cattcttaac tttgttgtgc tgaccgcttc gctgtcggca attaacagtg
    28981 acgtatttgg cgtaggccgt atgctccacg gtatggcaga gcagggcagc gcaccgaaaa
    29041 ttttcagcaa aacgtcgcgt cgcggtattc cgtgggttac ggtgctggtg atgactaccg
    29101 cgctgctgtt tgcggtgtat ctgaactaca tcatgccgga aaacgtcttc ctggtgatcg
    29161 cttcgctggc aaccttcgcc acggtatggg tgtggattat gatcctgctg tcgcaaatcg
    29221 ccttccgtcg ccgtttgccg ccagaagaag ttaaggcgct gaaatttaaa gtgccgggtg
    29281 gggtagcaac gaccatcggc gggctgattt tcctgctctt tattatcggg ttgattggtt
    29341 atcacccgga tacgcgtatc tcgctgtatg tcggtttcgc gtggattgtt gtgctgttga
    29401 ttggctggat gtttaagcgt cgccacgatc gtcagctggc tgaaaaccag taatccttgc
    29461 gttctgtaag ccggttcagg aggaaatcct gatccggcta ttccgaaagt tatccgcccc
    29521 cgtcctcctc ccccaaatat ccttcagatg atgagtgatc ctgcattagg ctatggcaag
    29581 gtgatcagat tttcatcaca ggggaattat gatgttaaat gcatggcacc tgccggtgcc
    29641 cccatttgtt aaacaaagca aagatcaact gctcattaca ctgtggctga cgggcgaaga
    29701 cccaccgcag cgcattatgc tgcgtacaga acacgataac gaagaaatgt cagtaccaat
    29761 gcataagcag cgcagtcagc cgcagccagg cgtcaccgca gggcttcagc cgaatgccgc
    29821 cggcacgact ggagcagttt gccgtcgatg taccggatat cggcccacaa tgggctgcgg
    29881 atcagatttt ttatcagatc ttccctgatc gttttgcgcg tagtcttcct cgtgaagctg
    29941 aacaggatca tgtctattac catcatgcag ccggacaaga gatcatcttg cgtgactggg
    30001 atgaaccggt cacggcgcag gcgggcggat caacgttcta tggcggcgat ctggacggga
    30061 taagcgaaaa actgccgtat ctgaaaaagc ttggcgtgac agcgctgtat ctcaatccgg
    30121 tgtttaaagc tcccagcgta catcaatacg ataccgagga ttatcgccat gtcgatccgc
    30181 agtttggcgg tgatggggcg ttgctgcgtt tgcgacacaa tacgcagcag ctgggaatgc
    30241 ggctggtgct ggacggcgtg tttaaccaca gtggcgattc ccatgcctgg tttgacaggc
    30301 acaatcgtgg cacgggggga gcttgttaca accctgaatc gccctggcgc gactggtact
    30361 cgtttagtga tgatggcacg gcgctcgact ggcttggcta tgccagcttg ccgaagctgg
    30421 attatcagtc ggaaagtctg gtgaatgaaa tttatcgcgg ggaagacagt attgtccgcc
    30481 attggctgac agcgccgtgg agtatggacg gctggcggct ggatgtggtg catatgctgg
    30541 gggaggcggg tggggcgcgc aataatatgc agcacgttgc tgggatcacc gaagcggcga
    30601 aagaaaccca gccggaagcg tatattgtcg gcgaacattt tggcgatgca cggcaatggt
    30661 tacaggccga tgtggaagat gccgccatga actatcgtgg cttcacattc ccgttgtggg
    30721 gatttcttgc caataccgat atctcttacg atccgcagca aattgatgcc caaacctgta
    30781 tggcctggat ggataattac cgcgcagggc tttctcatca acaacaatta cgtatgttta
    30841 atcagctcga cagccacgat actgcgcgat ttaaaacgct gctcggtcgg gatattgcgc
    30901 gcctgccgct ggcggtggtc tggctgttca cctggcctgg tgtaccgtgc atttattacg
    30961 gtgatgaagt gggactggat ggtaaaaacg atccgttttg ccgtaaaccg ttcccctggc
    31021 aggtggaaaa gcaggatacg gcgttattcg cgctgtaccg gcgaatgatt gtgctgcgta
    31081 agaaaagtca ggcgctgcgt cgtggcggct gtcaggtgct gtatgcggaa gataacgtag
    31141 tggtatttgt ccgcgtgctg aatcagcaac gtgtactggt ggcaatcaac cgtggcgagg
    31201 cctgtgaagt ggtgctaccc gcgtcaccgt ttctcaatgc cgtgcaatgg caatgcaaag
    31261 aagggcatgg gcaactgact gacgggattc tggctttgcc tgccatttcg gctacggtat
    31321 ggatgaacta acgttttata acgccttgtg tgacgcctgc gccatcatcc gcggataaaa
    31381 ctgccagaag cgggttccga gggcggtata atgggcgttt aaatcgtacc aggagtcacg
    31441 cagggcatcc agacgtgggc gacggcttgc catgccgttt aacacgttct ggatgaaatc
    31501 catatcgcga tagcgcacca gccactgctc tgaccacaag taattgttta gattgataaa
    31561 acgtggcggt gagtccggca aaatcgtcat cacttgcttg cgggcataac agataaattc
    31621 ctgtagcgga aagtccggcg acagctgcga ccagtggcgg gaaagaaagt gatcccacat
    31681 gacatccagc gtaataggcg caacgcggcg cgtttcacga cgaaaccact cccgtgcttc
    31741 gcggacttcc ggcagattgt cagtcaatac gtcgatacgt cgatgcatat gaatgccagc
    31801 cacgacgtcg ggcggaaaac tttcttcagg atttccgcgt acgaaatcag ccagtaaatt
    31861 gccggaaagc gagctttccg cgagatgggc taaatgcagg tgagctaaaa aattcatcga
    31921 ttatattcta tccaaagggg ggtaaaggtt gcagggagag cgccccggca ctagactacc
    31981 cgcctcttat tttagtctga gtcagtgtca tgcgcgttac cgatttcttc tttgaattgc
    32041 ccgaatccct gattgcccac taccccatgc ctgaacgcag tagctgtcgt ttactgtcgc
    32101 tggacgggcc tacgggcgcg ctgacgcacg gtactttcac cgatttactc gataagctca
    32161 accccggcga tcttctggtt tttaataata ctcgcgtgat cccggcgcgc ctgtttgggc
    32221 gtaaagccag cggcggcaag attgaagtgc tggttgagcg gatgctcgac gacaaacgca
    32281 ttcttgcgca tattcgcgcc tcgaaagcgc caaaacctgg cgcagaactg ctgctgggcg
    32341 atgacgaaag tattaacgca acaatgaccg cgcgccacgg cgcactgttt gaagtcgaat
    32401 tcaatgatga tcgttcggta ctggatatcc tcaacagtat cgggcatatg ccgctgccgc
    32461 cgtatatcga ccgtccggac gaagacgctg accgcgaact ttatcaaacc gtttatagcg
    32521 aaaaaccggg cgcggttgca gccccgaccg ctggtctgca ttttgacgag cctttgctgg
    32581 aaaaattgcg cgccaaaggc gtggagatgg cgtttgtgac gttgcacgtt ggtgcgggca
    32641 ccttccagcc ggtgcgcgtc gacaccattg aagatcacat catgcactcg gaatacgctg
    32701 aagtaccgca ggatgtggta gacgcggtac tggcggcgaa agcgcgcggt aaccgggtga
    32761 ttgcggttgg caccacttca gtacgttcgc tggaaagcgc ggctcaggca gcgaaaaacg
    32821 atctcattga accgttcttc gacgataccc aaatctttat ctatccgggc ttccagtaca
    32881 aagtggtcga tgcgctggtg acgaacttcc acttgccaga gtcgacgctg attatgctgg
    32941 tttcggcctt tgccggttat caacacacca tgaacgccta taaagcagcg gtagaagaga
    33001 aatatcgctt ttttagttac ggtgatgcga tgtttattac gtacaatccg caggcaatta
    33061 atgagcgcgt cggggagtaa ttccgcggcg ctggtttaaa acgttggact gtttttctga
    33121 cgtagtggag aaaaaatgaa atttgaactg gacaccaccg acggtcgcgc acgccgtggc
    33181 cgcctggtct ttgatcgtgg cgtagtggaa acgccttgtt ttatgcctgt tggcacctac
    33241 ggcaccgtaa aagggatgac gccggaagaa gttgaagcca ctggcgcgca aattatcctc
    33301 ggcaacacct tccacctgtg gctgcgcccg ggccaggaaa tcatgaaact gcacggcgat
    33361 ctgcacgatt ttatgcagtg gaaggggccg atcctcaccg actccggggg cttccaggtc
    33421 ttcagccttg gcgatattcg taaaatcacc gaacagggcg tgcacttccg taacccgatc
    33481 aacggcgatc caattttcct cgatcctgaa aaatcaatgg agattcagta cgatcttggt
    33541 tcggatatcg tcatgatctt tgatgagtgt acgccgtatc ctgctgactg ggattacgca
    33601 aaacgctcca tggagatgtc tctgcgttgg gcgaagcgta gccgtgagcg ttttgacagt
    33661 ctcggaaaca aaaatgcgct gtttggtatc attcagggca gcgtttacga agatttacgt
    33721 gatatttctg ttaaaggtct ggtagatatc ggttttgatg gctacgctgt cggcggtctg
    33781 gctgtgggtg agccgaaagc agatatgcac cgcattctgg agcatgtatg cccgcaaatt
    33841 ccggcagaca aaccgcgtta cctgatgggc gttggtaaac cagaagacct ggttgaaggc
    33901 gtacgtcgtg gtatcgatat gtttgactgc gtaatgccaa cccgcaacgc ccgaaatggt
    33961 catttgttcg tgaccgatgg cgtggtgaaa atccgcaatg cgaagtataa gagcgatact
    34021 ggcccactcg atcctgagtg tgattgctac acctgtcgca attattcacg cgcttacttg
    34081 catcatcttg accgttgcaa cgaaatatta ggcgcgcgac tcaacaccat tcataacctt
    34141 cgttactacc agcgtttgat ggcgggttta cgcaaggcta ttgaagaggg taaattagag
    34201 agcttcgtaa ctgattttta ccagcgtcag gggcgagaag taccaccttt gaacgttgat
    34261 taatattaat aatgagggaa atttaatgag cttttttatt tctgatgcgg tagcggcaac
    34321 gggtgcaccg gcgcaaggta gcccgatgtc tttgattttg atgctggtgg tattcggtct
    34381 gattttctat ttcatgatcc tgcgtccaca gcagaagcgc accaaagaac acaaaaagct
    34441 gatggactcc attgccaaag gtgatgaagt tctgacgaac ggtggcctgg ttggtcgcgt
    34501 aaccaaagta gcggaaaacg gctacattgc tatcgcgctg aatgacacca ctgaagtagt
    34561 tattaaacgt gacttcgtag ctgccgtcct gccgaaaggc accatgaagg cgctgtaatt
    34621 aaaatttttc cctaagggaa ttgccgtgtt aaaccgttat cctttgtgga agtacgtcat
    34681 gctgatcgtg gtgattgtca tcggtctgct gtatgcgctt cccaacctgt ttggtgagga
    34741 tccggctgtt cagatcactg gtgcgcgcgg agtcgccgcc agtgagcaaa cgctgatcca
    34801 ggtccagaaa acgttacaag aagaaaaaat aactgctaag tctgtggcac tggaagaggg
    34861 cgctattctt gcgcgcttcg actccactga cacccagctg cgcgctcgtg aagcattaat
    34921 gggcgttatg ggtgacaaat acgtcgtggc gcttaacctt gccccggcaa cgccgcgctg
    34981 gctggcagct attcacgctg agccgatgaa gctcggcctt gacctgcgtg gcggcgttca
    35041 cttcctgatg gaagtggata tggacaccgc gcttggcaaa ctccaggaac aaaatatcga
    35101 tagcctgcgc agcgatctgc gtgaaaaggg catcccgtat accaccgttc gtaaagaaaa
    35161 caactacggc ctgagcatta ctttccgcga tgctaaagct cgtgatgaag ccattgcgta
    35221 tctgagcaag cgccatccgg acctggtgat tagtagccag ggcagcaacc agctgcgtgc
    35281 ggtaatgagc gatgctcgtc tgagtgaagc gcgtgaatat gcggtgcagc agaacatcaa
    35341 tatcctgcgt aaccgtgtaa accaacttgg cgtagcggag ccggtggttc agcgtcaggg
    35401 tgctgaccgt atcgttgttg aactgccagg tattcaggac actgcgcgtg cgaaagagat
    35461 tctgggtgcg acggcaacgc tggaattccg tctggtaaac accaacgttg accaggccgc
    35521 tgcggcatcc ggtcgcgtac cgggcgactc tgaagtgaaa cagacccgcg aaggtcagcc
    35581 agttgtgctg tacaaacgcg taattctgac cggtgaccat atcaccgact ccacttccag
    35641 ccaggacgaa tacaaccaac cgcaggttaa catctcgctc gatagcgctg gtggtaacat
    35701 catgtctaac ttcactaagg acaacatcgg caaaccgatg gcaaccctgt ttgtggagta
    35761 caaagacagc ggtaagaaag atgcaaatgg tcgtgcggtt ctggtgaaac aggaagaggt
    35821 gattaacatc gccaacatcc agtctcgtct gggtaacagc ttccgtatca ccggcatcaa
    35881 caacccgaac gaagcccgtc agctgtcact gctgctgcgt gccggtgcgt tgatcgcgcc
    35941 gattcagatt gttgaagaac gtaccattgg cccaaccctg ggtatgcaga acattgaaca
    36001 ggggctggag gcttgccttg ccggtctgct ggtttctatt ctgttcatga tcatcttcta
    36061 taagaagttt ggtctgattg cgaccagtgc tctgattgcc aacttgatct taatcgtcgg
    36121 cattatgtcg ctgttgccag gcgcaacgct gagtatgcca ggtatcgcgg gtatcgtctt
    36181 aacccttgcg gtggcggtcg atgcgaacgt actgatcaac gaacgtatta aagaagagtt
    36241 gagcaacgga cgtactgttc aacaggcaat tgatgaaggt tatcgtggcg cattcagttc
    36301 tatcttcgat gcgaacatca ccacgctgat taaagtcatc atcctgtacg cagtgggtac
    36361 cggggcaatt aaagggttcg cgattactac cggtatcggt gtggcgacgt cgatgtttac
    36421 cgcgattgtc ggtacgcgtg ccatcgtaaa cctgctatat ggcggcaagc gcgtcaagaa
    36481 gctgtcaatc tgaggagtgc gatgtggcac aggaatatac tgttgaacaa ctaaaccacg
    36541 gccgtaaagt ctatgacttt atgcgctggg actactgggc tttcggcatc tctggtctgc
    36601 tgttaatcgc cgctatcgtt attatgggcg tgcgcggctt taactggggg ctggatttca
    36661 ccggtggtac ggttatcgaa attacgctcg aaaaaccggc tgaaattgac gtaatgcgtg
    36721 atgcattgca aaaagccggt tttgaagagc caatgctgca aaactttggt agcagccacg
    36781 acatcatggt ccgtatgccg cctgctgaag gcgaaaccgg cggtcaggtg ctgggcagcc
    36841 aggttctgaa ggtgattaac gaatccacca atcagaatgc agcagtgaag cgtattgagt
    36901 tcgtcggtcc gagcgtgggg gcagacctta cgcaaaccgg tgcgatggcg ttgatggcgg
    36961 cgctgctgtc tatccttgtg tacgtaggtt ttcgctttga gtggcgactg gcggcagggg
    37021 ttgttattgc gctggcgcac gacgttatca ttacgctggg tattttgtcg ttattccata
    37081 tcgagattga cctgaccatt gtggcatcgt tgatgtcggt tatcggttac tcgcttaacg
    37141 acagtatcgt ggtatcggac cgtattcgtg aaaacttccg caagatccgt cgcggtacgc
    37201 cttacgaaat ctttaacgtg tccttgaccc agacgctgca ccgtaccttg atcacatccg
    37261 gtactacctt gatggttatc ctgatgctgt acctcttcgg tggtccggta ctggaaggct
    37321 tctcgctgac catgcttatc ggtgtttcta tcggtactgc atcttccatc tatgtggcat
    37381 ctgcgttggc tctgaaactg ggtatgaagc gcgaacacat gttgcagcag aaagtggaaa
    37441 aagaaggggc ggatcagccg tcaattctgc cgtaatcaag ttcccgttga tgttgaaaat
    37501 cccggtcaga agatcgggat tttttttgat gtatggagat tgcgaaaatc cccgcatctt
    37561 gggaaactgc gcgtaaccct acatttcatc caggtaactc ttcatggcta tcatcccaaa
    37621 aaactatgcg cggttagaaa gcggctatcg cgaaaaagca ttaaaaatat atccgtgggt
    37681 ctgcggtcgc tgttcccgcg agtttgttta ttccaacctg cgtgaactta ccgttcacca
    37741 cattgatcac gaccatacca ataacccgga agatggcagt aactgggaat tgctttgtct
    37801 ctattgccac gatcatgagc attcgaaata taccgaagcg gatcagtatg gtacgaccgt
    37861 tatcgcaggg gaagatgcgc agaaagatgt cggtgaagcg aagtacaacc cattcgctga
    37921 cctgaaagcg atgatgaaca agaagaagtg attaaaacgt aaaattgcct gatgcgctac
    37981 gcttatcagg cctacgttat ttcagcaata tattgaattt acgtgctttt gtgggccgga
    38041 caaagcgttt acgccgcatc cggcatgaac aaagctcacg ttgttaacaa tcagaaatgc
    38101 cgggaataaa tcccggcatt ttcataatca gaagttgtaa cctactacca ggtaaccacc
    38161 ccagccggta gagcgaacgt tgaagttgcc gttgccgaag ttcagttctg catcgtcgtt
    38221 ccactgacca ccgtcgtgcc agtaacgagc tacgacagag tagtgccagt gatcgtagtt
    38281 cagagccaga atatggctgg aagcgataga gttattagta cgggtcttaa taccgttgat
    38341 tgcgttaccg ctgtcatccc ctaaatcgga accccagtcg aagttggtga agccgatgta
    38401 gctcagctga ccgccccaca gatcggtaat cggcacaaag tatttaattt tgaaacggta
    38461 accgtcccac tcgttttcgt tcgctgcgcc atagttctgc cactggtatt tcgcatagac
    38521 gttcatggac aggctcatcg gcagaccagt atcgatgtcg gtacccagac ccatgtacca
    38581 ggtgctctgg cgaccatttt tattacgacc catgtcgtaa atgtagttgt tcgcgaagta
    38641 ccactctttg aacggaccga agttaaggtc agtattggtc agcttgtcga tggagaaacg
    38701 tggttcgatt tccataaaca gcggagaacc gtggttccag atacctttag catcggagtt
    38761 accgccgaag aataccggcg catccgcata accatagaag tcgaaccagt cttttttagc
    38821 gaatgcttcg tactcaaggt aggtatcgtt gcggatctgc ggtccgaaac gggtgtgata
    38881 gcttccgaca acgttaacgc tctggtgcca ccagtcggaa agatactgcg gtttgtcgtt
    38941 ttcagctgcg ttgacagtaa aagacgaaga gagcgccagt accgcaccgg ctgccagtaa
    39001 tgtttttttc atatgtatgc cactgtttga aaatcccttg cgggagtgaa aaaggcgcaa
    39061 attgcgtttc taaatatttc gtatacagag gagcctatta taaaaatcat tgctcacaaa
    39121 aatatgtttc gtttcacagt tctatcattt acgtaatcga ttgcgttcac gtttacacac
    39181 attcgggcgg ggattgtact gactttcgcc cttgttgcaa acttttacta tcaatccagg
    39241 tgatgtaagc agagtaacaa aatgacgggg aaagatgttg cgggacgcac aaaatcgtcc
    39301 cgaaatacgt ttactgcata acaggttgaa tgtcgtggat acgtacaaag cctaactgat
    39361 ccacagaaat cccctttaat tgtaacggaa tatcaacatc gctgggggcg aggacgctgg
    39421 caggggcatt aatcaattga ttctgcacat tgatttcctg aaagttgtct attgttccct
    39481 gaatttgccc atattcaaca gttccgctga atgcgggcaa gggatcattc gactcaccct
    39541 gaatacgtag tgtaagggtg gtgccatctg tatcaggcgt aatattcacc agcgacatac
    39601 gtaaagtacc gatctggctt tccagtcttg ccggtgtttt cgctccaggc agaaggtaac
    39661 ccccactgct ggattttgca ttcagcctgt tttgctgagt gattttgact gtttcttgat
    39721 tgagttgcgt catctcctgg ttcagcgtgc tgacgctgtg tttcatctgt cgcacttcac
    39781 tttgctgcac acaggcgcta aggctgaaca ggcttcccag caggagcagt cgaaaaacgt
    39841 tagtattcat aggtcgtccc tttctcgtcg caattagcct aatggtagag ggtaaaagca
    39901 gcgaaagcat cgatccttta tctcaaaagc gttgcgcctt tgttgtatcg tcagttcagg
    39961 gtaaaataga tttccgttaa ccacctggtc aggacgccgt atgcattgcc cattctgttt
    40021 cgccgtggac actaaggtaa ttgactctcg tctcgtgggc gagggttcat ccgtacgccg
    40081 ccgtcggcag tgtctggtgt gtaatgaacg tttcaccacc tttgaagtgg cggagctggt
    40141 tatgccgcgt gttgtaaaaa gcaacgacgt gcgtgaaccg tttaatgaag agaaattgcg
    40201 tagcggaatg ctgcgggcgc tggaaaaacg tccggtgagt tccgatgacg ttgaaatggc
    40261 aatcaatcat attaaatcgc agctgcgcgc caccggtgag cgcgaagtac cgagcaagat
    40321 gattggcaat ctggtgatgg agcaattgaa aaagctcgat aaagtcgcct atatccgttt
    40381 tgcctctgtc taccgcagtt tcgaagatat caaagaattt ggcgaagaga tcgcgcgcct
    40441 ggaggactaa gccgtgcagg acgagtatta catggcgcgg gcgctaaagc tggcgcaacg
    40501 aggacgtttt accacccacc ccaacccaaa tgtcgggtgc gtcattgtca aagatggcga
    40561 aattgtcggt gaaggttacc atcaacgagc gggtgaacca catgccgaag tacacgcgtt
    40621 gcgtatggcg ggtgaaaaag ccaaaggtgc aacggcgtat gtgacactcg aaccttgcag
    40681 ccatcacggc cgtacgccgc cttgctgtga tgctttaatt gccgcaggtg tggcgcgcgt
    40741 ggttgcctcg atgcaagatc ctaacccgca ggtcgctggg cgtggacttt accgtctgca
    40801 acaggctggc attgacgtca gccacggcct gatgatgagt gaagccgagc aattgaataa
    40861 aggctttctc aagcggatgc gcaccggctt tccttatatt cagttaaaac ttggcgcatc
    40921 gcttgatggt cgcacggcga tggcgagcgg cgaaagccag tggatcactt cgccccaggc
    40981 gcggcgtgat gtacaacgac tgcgcgcgca aagtcatgcc attttaacca gcagcgccac
    41041 ggtgctggcg gatgatcctg ccttaacggt gcgttggtcc gaactggatg aacaaactca
    41101 ggcgctctac ccgcaacaaa atctccgcca gccggtacgt attgtgattg atagccaaaa
    41161 tcgcgtgacg ccggaacatc acattgtgca gcagcccggc gaaacctggt tcgcgcgtac
    41221 gcaggaagat tctcgtgagt ggccggaaac ggtgcgtacc ttgctgattc cagagcataa
    41281 aggtcatctg gatctggttg tactgatgat gcaactgggt aaacagcaga ttaacagcat
    41341 ctggggggaa gcggggccaa cgctcgctgg cgcattgctg caggcgggtt tagtcgatga
    41401 gctgattgtc tatatcgcac ctaaactatt aggcagcgac gcccgtggat tatgcaccct
    41461 gccagggctt gagaaattag ccgacgcccc ccaatttaaa ttcaaagaga tacgtcatgt
    41521 aggcccggat gtttgcctgc atttagtggg tgcatgatct cccggctcga aagggaagca
    41581 gcgcacgaaa tattatgcta aaatccgccc ccctgcgggg ccatactcga acccgaagga
    41641 agaaaatgaa cattattgaa gctaacgttg ctaccccgga cgctcgcgtc gccatcacca
    41701 tcgcgcgttt caacaacttt atcaatgaca gcctgctgga aggtgcaatt gacgcactga
    41761 aacgtatcgg tcaggtaaaa gatgaaaaca ttaccgttgt ttgggtgcct ggtgcctatg
    41821 agctgccgct ggcggcgggt gcactggcta aaaccggtaa atacgacgcg gtgattgcgc
    41881 tgggtacggt tattcgtggt ggcactgccc attttgaata tgtcgctggt ggtgcaagca
    41941 acggcctggc gcatgttgcc caggacagcg aaattccggt tgcttttggg gttctgacca
    42001 ctgaaagcat tgaacaagcg atcgaacgtg ctggcaccaa agctggcaac aaaggtgcag
    42061 aagctgcact gaccgcgctt gaaatgatta atgtattgaa agccatcaag gcctgaaatt
    42121 agtaagggga aatccgtgaa acctgctgct cgtcgccgcg ctcgtgagtg tgccgtccag
    42181 gcgctctact cctggcagtt gtcccagaac gacatcgctg atgttgaata ccagttcctg
    42241 gctgaacagg atgtaaaaga cgttgacgtc ctgtacttcc gtgagctgct ggccggggtg
    42301 gcgactaata ccgcatacct cgacggactg atgaagccat acctgtcccg tctgctggaa
    42361 gaactgggac aggtagaaaa agcagtactg cgcattgcgc tgtacgaact gtctaaacgt
    42421 agcgatgtgc catacaaagt ggccattaac gaagcgatcg aactggcgaa atcgttcggc
    42481 gcagaagaca gccataagtt cgtcaacggt gtactcgata aagcagcacc tgtgattcgc
    42541 cctaacaaaa agtaatatcc aggccggtag attcacggaa gaccgttcca tgatcgccgg
    42601 ccttttcttt tttacctgct gaggcataac gtatggcatg tggcgagttc tccctgattg
    42661 cccgttattt tgaccgtgta agaagttctc gtcttgatgt cgaactgggc atcggcgacg
    42721 attgcgcact tctcaatatc cccgagaaac agaccctggc gatcagcact gatacgctgg
    42781 tggcgggcaa ccacttcctc cctgatatcg atcctgctga tctggcgtat aaagcactgg
    42841 cggtgaacct aagcgatctg gcagcgatgg gggccgatcc ggcctggctg acgctggcat
    42901 taaccttgcc ggacgtagac gaagcgtggc ttgagtcctt cagcgacagt ttgtttgatc
    42961 ttctcaatta ttacgatatg caactcattg gcggcgatac cacgcgtggg ccattatcaa
    43021 tgacgttggg tatccacggc tttgttccga tgggacgagc cttaacgcgc tctggggcga
    43081 aaccgggtga ctggatctat gtgaccggta caccgggcga tagcgccgcc gggctggcga
    43141 ttttgcaaaa ccgtttgcag gttgccgatg ctaaagatgc ggactacttg atcaaacgtc
    43201 atctccgtcc atcgccgcgt attttacagg ggcaggcact gcgcgatctg gcaaattcag
    43261 ctatcgatct ctctgacggt ctgatttccg atctcgggca tatcgtgaaa gccagcgact
    43321 gcggcgtacg tattgacctg gcattgctgc cgttttctga tgcgctttct cgccatgttg
    43381 aaccggaaca ggcgctgcgc tgggcgctct ctggcggtga agattacgag ttgtgtttca
    43441 cggtgccgga actgaaccgt ggagcgctgg atgtggctct cggacacctg ggcgtaccgt
    43501 ttacctgtat cgggcaaatg accgccgata tcgaagggct ttgttttatt cgtgacggtg
    43561 aacctgttac gttagactgg aaaggatatg accattttgc cacgccataa agatgtcgcg
    43621 aaaagtcgcc tgaagatgag taatccgtgg catctacttg ctgtcggatt cggaagtgga
    43681 ttaagcccga tcgttcctgg gacgatgggc tcgctggcag cgattccgtt ctggtatctg
    43741 atgacctttt tgccctggca gctctactcg ctggtggtga tgctggggat ctgtatcggc
    43801 gtctatcttt gtcatcagac cgcaaaagat atgggcgtgc acgatcacgg cagcattgtc
    43861 tgggacgaat ttattggtat gtggatcacg ctcatggcgc tgccgaccaa tgactggcag
    43921 tgggttgccg ccgggtttgt gattttccgt attctggata tgtggaaacc gtggccgatc
    43981 cgctggtttg atcgcaatgt gcatggcggc atggggatca tgatagatga tattgtcgcc
    44041 ggggtgatct ccgctggcat cctgtacttt attggtcatc actggccgct gggcattctg
    44101 tcgtagttgt gcaccgatgc ctgatgtgac gcttgttgcg tctcatcagg cctggactct
    44161 tatttaaatc ctactaccgg atgcggttta tacggcgttt ccagttcggc aatctgttcc
    44221 ggcttcaaag tgatatccac cgcgttcaat agctcatcaa gctgttcttc ccgcgatgta
    44281 ccgataatcg gtgcggcaat gcccggttta ctcaacaacc aagccagcgc aacttgtgct
    44341 cgtgttgcac cgagttcttc actgacgccc gttaaccgct ctgcgatctg cgcgtcattt
    44401 tcatcgcttt ctttatagag atttttcccc acctcatcag acaccagtcg tgcggtagtt
    44461 tctccccacg gacgcgtcag tcgcccccgc gccagcgggc tccacggaat caccgccacg
    44521 ccctcctgat aaaacagtgg cagcatctcg cgctcttctt cgcgataaat cagattgtag
    44581 tgatcctgca tactgacaaa ctgcgcccag ccgtgctgtt tttggagttc cagtgcctga
    44641 gcaaactgcg aagcgtgcat tgatgacgcg ccgatataac gcgctttccc ggcttttacc
    44701 acgtcgttca gggcttccag cgtctcttcg atcggcgtgt tgtaatccca gcgatgaatt
    44761 tgcaggatat cgacataatc catgccgaga cgtcgcaggc tgtcgtcgat agagcgcaaa
    44821 atttgtgcac gggataatcc ttccggtaaa tcaccaacgc gatggaacac tttggtggca
    44881 acgaccacgt cttcacgacg ggcgaaatcc cgcagtgcgc gaccaacgat ctcttcgctg
    44941 ctaccatcgg aatagctatt ggcggtatca aagaaattta tgccgccttc cagtgcgcgt
    45001 ttaattatgg gacggctgct ctcttccggc agtgtccatg cgtgattacc acgatctggc
    45061 tcgccaaagg tcatacagcc gaggcaaagt cgggaaacgc gaaggtcggt ttttcctaag
    45121 gggttgtatt gcatgctgcc actcctgcca tgaataattt cttaagcata gcaggagtgg
    45181 agtagggatt atgccagcca ggccttgatt ttggcttcca taccggcggc atcgaggccg
    45241 agttcggcgc gcatttcttc ctgcgttcct tgcggaataa agaagtcagg caggccaatg
    45301 ttcagcacgg ggactggttt acgatgggcc attagcactt cgttcactcc gctgcctgca
    45361 ccgcccataa tggcgttttc ttctacggta accagtgctt catgactggc ggccatttcc
    45421 agaattaacg cttcatcaag cggtttcaca aaacgcatat cgaccagcgt ggcgttcagc
    45481 gattcggcga ctttcgccgc ttctggcatc agcgtaccaa agttaaggat cgccagtttc
    45541 tcgccacgac gcttcacaat gcctttgcca attggtagtt tttccagcgg cgtcagttcc
    45601 acgccgaccg cgttgccacg cgggtagcgc accgctgacg ggccatcgtt atagtgatag
    45661 ccggtataga gcatctggcg acattcgttt tcatcgctcg gggtcataat gaccatttcc
    45721 ggtatgcagc gcaggtaaga gagatcaaaa gcaccctgat gggtttgacc gtcagcacca
    45781 acaatgcccg cgcggtcgat ggcgaacagg accggaagct tttgaattgc cacgtcatgc
    45841 agcacctgat cataggcgcg ttgcaggaaa gtggagtaga tcgcgacaat gggtttgtac
    45901 ccaccaatcg ccagaccggc ggcaaaggtc actgcgtgtt gctcggcaat tgccacgtcg
    45961 aagtagcgat ccgggaattt acgagaaaac tcgaccatgc cggaaccttc acgcatcgcc
    46021 ggagtaatcg ccatcagctt gttatctttc gccgccgttt cgcacaacca gtcgccaaag
    46081 atttttgaat agctcggtaa accgccgcta cttttcggca aacaaccgct ggagggatca
    46141 aatttaggca cggcgtggaa agtgattggg tctttttctg ccggttcata accacgacct
    46201 tttttggtca tgatatgcag gaactgcggg cctttcaggt cacgcatgtt cttcagcgtg
    46261 gtgataagcc ccagcacatc gtgaccgtca accgggccga tgtagttaaa gcccagctct
    46321 tcaaacaacg tgccagggac taccatgcct ttaatatgtt cttcggtacg tttgagcagc
    46381 tctttaatgg gtggcacgcc agagaaaact tttttcccgc cttcgcgcag tgaagagtaa
    46441 agcttaccgg aaagcagctg cgccagatgg ttgttgagcg cgccgacatt ttcggaaatc
    46501 gacatttcgt tgtcgttgag gaccaccagc atatcaggac ggatatcgcc cgcgtgattc
    46561 atcgcttcaa acgccatgcc tgcggtaatc gcgccatcgc cgattacaca gacggtacgg
    46621 cgatttttgc cttctttctc ggcagcaacc gcaataccaa ttccggcact gatggaggtt
    46681 gatgaatgcc cgacgcttaa tacgtcatat tcgctttcgc cgcgccacgg gaacgggtgc
    46741 aggccgcctt tctgacggat ggtgccgatt ttgtcgcggc gtccggtcaa aattttatgc
    46801 ggataagcct gatgccccac atcccaaatc aattggtcaa acggggtgtt gtagacatag
    46861 tgcagcgcca cggtcagttc gaccgtgccc agcccggagg cgaagtgccc gctggaacgg
    46921 ctcacgctgt cgagtaaata gcggcgcagt tcgtcgcaga gtttcggtaa actctctttc
    46981 ggcaacagtc gtaactcctg ggtggagtcg accagtgcca gggtcgggta tttggcaata
    47041 tcaaaactca tcaggggcct attaatactt attgtttatt tattacgctg gatgatgtag
    47101 tccgctagcg cttccagtgc cgaggtatcg ggtgactgtt cagccagttg tttcagcgac
    47161 tgacgggcat cgtcgatcag atcccgggct ttcttccggg cttgctcaag acccagaagt
    47221 gcagggtagg tacttttacc aagttgctgg tcggcaccct ggcgttttcc caacgttgca
    47281 gtatctccta ccacatccag gatgtcatcc tgaacctgga aggcaaggcc gatgctctct
    47341 gcgtacttgt cgagtactgg cagagcacga cgccctttat ctccggcgct taatgcacca
    47401 agacgaacgg cggcgcgaat caatgcgccg gttttatgac gatgaatacg ctcaagcgcg
    47461 tccagaggta cgtgtttgcc ttccgcgtct aaatctaatg cctgaccacc gcacattccg
    47521 gcaataccgc tggcgctcgc cagttcagaa atcatcgaaa ttctgtcgcg atccgacact
    47581 tccggcatat cagcatcgct taaaatcgag aacgccagcg tttgtaaagc gtcgccagcg
    47641 agaatcgcgt ttgcttcgcc aaacttcaca tggcaggtcg gcaaaccgcg acgcagatcg
    47701 tcatcatcca tcgccggtaa atcatcatga attaatgagt aagcgtggat acactctacg
    47761 gcggcagcgg gtgcgtccag cgtgtttgtg ctaacgccaa acatatgacc ggtggcataa
    47821 accaggaagg gtcgcagacg cttaccacct aataatgcac catactgcat ggtttcgacc
    47881 acgggagtgt tctgaaaggg cagtggggcg ataaaacggc tcagcgcctg gttggcctgc
    47941 ttaacgcagg cttcgagttg ctgcggaaag tccattactc attgtccggt gtaaaagggg
    48001 ttagagaggc gtcttcattg tcagacagca gaatttgtac gcgctgttcg gcttgttgta
    48061 atttggcctg cccctgacgt gccagctgca cgccgcgttc gaactcgttc agcgcctctt
    48121 ccagcggcag gtcgccactt tccagacggg ttacaatctg ttccagctcg ctcagcgcct
    48181 tttcaaagct ggcgggagcc tcatttttct tcggcataat gaatgtctga ctctcaatat
    48241 ttttcgcccc gtcatggtaa cggactcagg gcaaatagca aataacgcgc aatggtaagg
    48301 tgatgtgcgc agcaaagcga tgttagtggt atacttccgc gcctggatgc agccgcaggt
    48361 gtgggctgct gtatttttcc ctatacaagt cgcttaaggc ttgccaacga accattgccg
    48421 ccatgaagtt tatcattaaa ttgttcccgg aaatcaccat caaaagccaa tctgtgcgct
    48481 tgcgctttat aaaaatcctt accgggaaca ttcgtaacgt tttaaagcac tatgatgaga
    48541 cgctcgctgt cgtccgccac tgggataaca tcgaagttcg tgcaaaagat gaaaaccagc
    48601 gtctggctat tcgcgacgcc ctgacccgta ttccgggtat ccaccatatt ctcgaagtcg
    48661 aagacgtgcc gtttaccgac atgcacgata tttttgagaa agcgttggtt cagtatcgcg
    48721 atcagctgga aggcaaaacc ttctgcgtac gcgtgaagcg ccgtggcaaa catgatttta
    48781 gctcgattga tgtggaacgt tacgtcggcg gcggtttaaa tcagcatatt gagtccgcgc
    48841 gcgtgaagct gaccaatccg gaagtgactg tccatctgga agtggaagac gatcgtctcc
    48901 tgctgattaa aggccgctac gaaggtattg gcggtttccc gatcggcacc caggaagatg
    48961 tgctgtcgct catttccggt ggtttcgact ccggtgtttc cagttatatg ttgatgcgtc
    49021 gcggctgccg cgtgcattac tgtttcttta accttggcgg cgcggcgcat gaaattggcg
    49081 tgcgtcaggt ggcgcattat ctgtggaacc gttttggcag ctcccaccgc gtgcgttttg
    49141 tcgctattaa tttcgaaccg gtcgtcgggg aaattctcga gaaaatcgac gacggtcaga
    49201 tgggcgttat cctcaaacgt atgatggtgc gtgccgcgtc taaagtggct gaacgttacg
    49261 gcgtacaggc gctggtcacc ggcgaagcgc tcggccaggt gtccagtcag acgctgacca
    49321 acctgcgcct gattgataac gtctccgata cgctgatcct gcgtccgctg atttcttacg
    49381 acaaagagca catcatcaac ctggctcgcc agattggcac cgaagacttt gctcgcacga
    49441 tgccggaata ttgcggtgtt atctccaaaa gcccgacggt gaaagcggtt aagtcgaaga
    49501 ttgaagcgga agaagagaaa ttcgacttca gcattctcga taaagtggtt gaagaagcga
    49561 ataacgttga tatccgcgaa atcgccctgc agaccgagca ggaagtggtg gaagtggaaa
    49621 ccgtcaatgg cttcggcccg aacgacgtga tcctcgatat ccgttctatc gatgaacagg
    49681 aagataagcc actgaaagtc gaagggattg atgtagtttc tctgccgttc tataaactga
    49741 gcaccaagtt tggcgatctc gaccagaaca gaacctggct actgtggtgt gagcgcgggg
    49801 tgatgagccg tctgcaggcg ctctatctgc gcgagcaggg ctttaacaat gtgaaggtat
    49861 atcgcccgta atttgtggtt gtaacgtcgc atctggtcag atgcgacgct taccgcatcc
    49921 gacactactc gtaataatta taaatccctg ccgccatcac cagttgtgat gccacttcat
    49981 gggctttctc gcgcccaacc tgcaggtcga taattttcag accaaagtcg atagctgtcc
    50041 ccggcccctg gctggtcagc aattttaccc gtgcatccca gacgacgcgc ttgtcctgcc
    50101 attgttcagc gggaattttg tctttcagcg tcgggaagcc ggtcatatta ccaatcggga
    50161 agatatcgtg cggcaccagc acggtggctg gcgcggcgca aatagccgcg acgatacgcc
    50221 cggaacggtg gaactgttta acggtttcaa ccagcagagt gctatcgcga aaacactctg
    50281 cgcctttaat gccaccaggc agtacgatca cgtcatattc gccatcagcc acctcgacca
    50341 gcggcgcatc cgccagcagc ttcacgccgc gcgagcaggt aattgccagg ttgccatcgc
    50401 tggctacgct ggcagtggtg actttgatac cgccgcgaac caagaggtcg atcgtggtga
    50461 cggcttcagt ctcttcacta ccaggggcga ggcaaaccag tgccgatgcg ctcatattca
    50521 ctctcctttc tttttaccat ttcaaacagg cgggtgtttt ccggtacggc aatcccatgc
    50581 gcgcgggcgc ggcgtaagag aaaaccattg atatagtcga tttcggtgtg gcgcagcgcg
    50641 cggatatcct gcaacatcga cgagatattt tccgctgtgg catcaatcac ctgcatcacg
    50701 taatcacgca aatcttccgc tgaagtatga tgcccttcgc gttcgatcac tgccgcgact
    50761 tcttcgcata tttgcataat ttcttgcgga tgatgacgta attcaccgtt cgggcaattc
    50821 cagatggcgg tcagtggatt aatcacgcag ttgactgcca gcttgcgcca cagctcggcg
    50881 cgaatattgt tatgccaggc aacgtcaggc aacacggttt gtaaaatatc cgccagataa
    50941 ctgtaatcgc catcctgttg ccgtgccgga ccaatatgcg tgataccgtt tgccacatga
    51001 ataatgacat tgccgtcgcg gcgggcggca tgggtggtgg tgcccatcag taatggctgc
    51061 tgaatatttt gcaactcttc gatggtgccc atgccgttgt gaattaacag tattggcgtg
    51121 gttacaggca gtgtggacgc gaggcttttg acggcatcgg aaacctgcca tgctttcagc
    51181 gtcaccagga gcagatcgct ggtggcgaga aaatcgggat cgttggcggt cagcgattcg
    51241 ttaaatatcg aaccatctgt ctcaaccaga ttcgcactac aataaggttg cggtacgcgc
    51301 agccagccct gaacttcatg accctgtttg caaagtgctg taagccataa ttgccctaag
    51361 gcaccgcatc ccaatacggt aattttcatt gttcctcctc acccgcaacc actccgggtg
    51421 ttcaataagg ctatccctta attgtgcatg ctgttgcgac tatgcacaat taagggatac
    51481 gtcctggtgc aggactgtcg gttatttaac tttgcgggta ttatgcttcg catcaaaaat
    51541 gaagggagag gaaaagatgc catctttcga tattgtctct gaagttgatc ttcaggaagc
    51601 acgtaacgcg gtcgataacg cgagccgcga agtggagtcc cgttttgact tccgtaacgt
    51661 tgaagcctca tttgagctga acgacgccag caaaaccatc aaagtgttga gcgagtccga
    51721 tttccaggtc aatcagttgc tggatattct gcgtgccaag ctgctgaagc gcggcattga
    51781 aggcagttca ctggatgtac cggaaaatat cgttcatagc ggtaaaacct ggtttgtgga
    51841 agcgaaactg aagcagggca ttgagagcgc gacccagaaa aaaatcgtca agatgatcaa
    51901 agacagcaaa ctgaaagtgc aggcgcaaat tcagggcgat gagatccgcg tgactggcaa
    51961 atctcgtgat gatttgcagg ctgtcatggc gatggtgcgc ggtggcgatc tgggtcagcc
    52021 gttccagttc aaaaacttcc gcgattaatc gcgacaacct gcgctttgtt catgtcagat
    52081 gcggcatgaa cgcctggttc ggcctacaaa ggcttgtaaa tcaggagctt agtaggcctg
    52141 atgagcgtag cgcagcaggc aatttcgcct cgcttttatg cctggcgaat tgcctgttca
    52201 acatcaaagc gattcgtcac tttgctgtcg attttcacat aagctgaatg ttcttcttct
    52261 gcaatcaaca cttctttgat gccttcagtt tctagcaaac gcacttttaa cgcctcgttt
    52321 gcggcaatgt tcgccgggat ttcaatgcgc aaactgctga catacgccgg ttctttcatg
    52381 gtactggcga ctgccagcca cactgcggcc agcattgcgc cagcgagaaa tacgccctga
    52441 ccgtcaaaca tgccgtcaat ccagccgccc agtgaaccgc caatcgccac gccaagaaac
    52501 tggctggtgg agtaaacacc catcgctgta cctttgtaac ctgctggcga ctctttactg
    52561 ataagtgaag gcagaagggc ttccatcaaa ttaaacgcta caaagaaaag ctgcacgccg
    52621 accaccagtt gccagaactg cgtttgcgcg ttccacaaca caatttccgc aaccacgatc
    52681 aatccgacgc agaagacaaa gacttgcttc attttgcgct taacttcagc gtagataatg
    52741 aaaggcacga ccgagccaaa ggcgattagc attgtcgcca gatagacctt ccagtgttca
    52801 gccgccggga accctgcatc agccagttgt ccgggcaggg caacaaacgt cgacatcagc
    52861 aaaatatgca gacacataat gccaaagttg agtttcagca gccgcggttc cgccagcact
    52921 ttactgaaac tgcctttcac cattccggac tcacgattaa gtacgtgagt gctactgttg
    52981 ggcacaaccc aaatggtcaa cgcaatgccg gtcgttgcca gaatagcgat catccagaac
    53041 agcgcgtgca gcccaagttt gtgagtgatg atcgggccaa gcaccatcgc aatggcaaag
    53101 gtaatgccaa agctcacgcc gataaacgcc attgctttgg tgcggttttg ttcgcgcgtg
    53161 agatcggaaa gcagcgccat aacggcagcg gcaatcgcac cggagccttg tagcgcccgg
    53221 cctagaataa ttccccagat ggagtcagag agcgcagcga taacgctacc ggcggcaaac
    53281 accgccagcc caccgacaat taatggtttg cgaccaatac ggtcggaaag caggccaaac
    53341 ggaatctgaa aaacggcctg agtcagacca taaataccaa tggcaatacc gattaatgct
    53401 tcgctggcac cttgcagagc catgccatac gtggtcagaa ccggcagaac cataaacatg
    53461 cccagcatgc gcaacgagaa tacggtccct aaaccccagg tcgcgcgcct ctcacctggc
    53521 gtcattttat aatcgttcat taccacctct gttttaaatt cgcgactagt gtaaagcggc
    53581 aaacgaatgg ggtaaacatg cggatttttt agtaaatatt tcgagaggga tatgtttcta
    53641 atgctaagaa aaaaggtgcc gtagcacctt tttaatagag aggttttgtt accacacagc
    53701 agccagcagc gtatgcgagt ccggtaccat aaaatcaacg gacatcatca ccgagagggc
    53761 agtgatggcg atgatagaga agccgaacag cttgcgcgcc cagattctgt catcagcaac
    53821 tttataaccg cgcagagcca tacctaacca ccagacgcta accgccgcgg cgaccaccag
    53881 atatttatat ccagcgtaac cgccaagaga gagcatcagc gtggcaacgg caaaggcgat
    53941 gatatacagc gtgatgtgat tcttcgccac cgaaatgcct tttaccactg gcaataccgg
    54001 aatgtttgcc gcctggtaat ccttaaagcg gaaaatggcg atggcatagg agtgaggcat
    54061 ctgccacagg ctgaagatag ccagcaggat cgctgcgccg ctatcgaact cgccggttac
    54121 tgcacagtag ccaatcaccg gcggcgcagc gccggagagc gaaccaatca atgtgccgta
    54181 gactgagtgg cgtttcatgt acaggctata gacgccgaca taaaccacaa agcccatcac
    54241 ccccagccag caggccagcg gattcgcgcc aaaccacagc agcataaagc cagcaatacc
    54301 cagcaaggtg gcgtacacca gcgagacagc aggagagatc aggcctttaa ccagcacccg
    54361 attcttcgtc ctttccatct ttctgtcgat atccctgtcg atgtagttgt taaacacaca
    54421 acccgacgcc acaaccagtg acaccccaac cagcgtgtag ataaacaggg gataatcaat
    54481 gctgcccttt gaggccagca ggaatccccc aatcaccgag atcaggttgc caaagatgat
    54541 gcctggtttc gttacttgca ggtattgctt aaacatcata accgccgctc ttagtgcatc
    54601 atcatgttgt agttgaggtt ccacataatc cagatggagc ctacaaccag gatagcgatg
    54661 attagcacgg tgaagacaaa cgccgtcatg ttccagcctt catctgattt ggtattcatg
    54721 tgcaggaagc acaccagatg caccagaacc tgtaccactg ccattgccag gattgttccc
    54781 agaattacgg ccggagaggc agcccctgtc atcaccatcc agaacggaat caccgtcaga
    54841 atgatcgaca ggataaagcc tgtcatgtag gtttttacgc tgccatggga cgcgccgctg
    54901 tgatcggtag aatgactcat tacatcgccc ccatcagata aacaacagtg aacacacaga
    54961 tccaaaccac atccaggaag tgccagaaca ggctcaggca catgatgcgg gtacggttag
    55021 tgctggtcag gccgcgacga gcgatttgta ccatcagcac cgccatccag ataagaccgg
    55081 aagtgacgtg cagaccgtgc gtgccgacca gcgcaaagaa cgctgacagg aagccgctgc
    55141 gatccggacc catgccgtta acaatcaggt gatggaattc gtagatttcc atcccgataa
    55201 atccggcacc aaacaaacag gtcaacgcca gccaggagat aacctggctt ttgttgtttt
    55261 tgtacatggc gatagccgcc atgccgtagg tgatggagct gaacaacagc aagaaagttt
    55321 caaccagaac gaacggcagt tcgaaaatgt ccttacctgt cgggccgcct gcggtgccgt
    55381 tcaccagaac ggcataggta gcaaacaaga ttgagaacag aatgcagtcg ctcatcaggt
    55441 agatccaaaa tccgaagatt ttggttccgc ctgcatcgtg gtgcccgtgt tcgtgcgcgt
    55501 gggcagtcgc gtgcgtcaaa gtatcagttg ccatttttca gccctgcctt agtaatctca
    55561 tcgaaatgct ggttttccag tttttcgatt tctgccaccg gcacgtagta atccacgtcc
    55621 tcgtcgaagc ttttcacgat ccaggtgatg atcatgcctg cgaagccaac aatcgccagc
    55681 caccagatat gccagatcat ggcgaaaccg aagatggtgg agaaagctgc aatgacgata
    55741 cctgcaccgc tgtttttcgg catatgaatt tcttcatagt ggtcaggctt tttatacgct
    55801 tcgcctttct ctttcatttc ccagaatgca tcacgctcgt gaacgtgcgg cacaacggca
    55861 aagttataga acggaggcgg ggaagagttt gcccactcca gcgtacggcc accccacggg
    55921 tcgccagtca ggtcacggtt ctggtcgcgg tcgcgaatag aaacgtacat ctgaataacg
    55981 aggcagagaa tacccagcgc aatcagtact gcaccgctag ctgcaatcat cagcatggtg
    56041 tggaactgcg ggtcaatctg ctggctcaaa cgacgggtca tgcccatgaa gcccagtgca
    56101 tacagcggca taaaggcaac gaagaagccg atgatccaga accagaacgc gcgtttaccc
    56161 caggtttcat tcagtttgaa accgaacgct ttaggccacc agtaggtcat ccctgcgaag
    56221 cagccgaaga ccacgccgcc gatgataacg ttatggaagt gcgcaatcag gaacaggctg
    56281 ttatgcagaa cgaagtccgc gcccggtact gccagcagca cgccagtcat cccgcccacc
    56341 gagaaagtga cgataaaacc gatggtccac agcatcgcag aatggaacac gatacggccc
    56401 tgatacatgg tgaacagcca gttgaagatc ttcaccccgg tcgggatggc gataatcatt
    56461 gtggtgatac caaagaaggc gtttacgttc gcgcccgcac ccatcgtaaa gaagtggtgc
    56521 agccaaacga tgaacgacag cacggtgata cagacggttg cccataccag cgaggtataa
    56581 ccaaacagac gtttacgcga gaaggttgcc gcaatttcgg agaacacacc gaaaacaggc
    56641 aggatcagga tgtaaacttc cgggtggccc caggcccaaa tcaggttgat gtacatcatc
    56701 atgttgccac ccatatcgtt ggtaaagaaa tgggtgccca gatagcgatc cagggtcaac
    56761 aacgcgacgg taaccgtcag aattgggaag gaagcaataa tcagtacgtt cgcgcacagt
    56821 gatgcccagg taaatactgg catcttgaac atggtcatgc ccggtgcgcg catcttcaga
    56881 atggtaacga agaagttgat accggtaagc gtcgtaccta taccggatag ctggagactc
    56941 catatccagt aatcaacacc gactcccgga ctgtactcta ttcccgatag cggtggatag
    57001 gccagccagc cggtctgcgc aaattcgccc acgccgagag aaacgttaac cagaatcaca
    57061 ccaacaacag taaaccagaa gcttaagttg ttgaggaacg ggaacgcaac gtcacgcgcg
    57121 ccgatctgca gcggaaccac caggttcatc agaccgataa cgaaaggcat cgccacgaag
    57181 aagatcataa tcacgccgtg cgcggtgaag atctgatcgt agtggtgagg tggcaggaag
    57241 cccgcttcgc ccgccgaggc aagagcctgc tggctacgca tcataatggc gtcagcaaaa
    57301 ccacgcagca acatcacaat cgccacgatg atatacatga taccgaggcg tttatggtcg
    57361 acggaggtca gccactcttt ccacaggtag gtccacttac cgaagtaagt gatcaggcca
    57421 acgagcgcca gacctcccaa aataatgcca gcgatcgtaa ccatgacgat aggttcatgg
    57481 aacgggactg catcaagtga taattttccg aacatcttta ttcttcctca acccctttaa
    57541 tgggcggatt ccgcgtggct catgtccatg ccttccatac cttcgtgtgc gctgtgctca
    57601 ccttctggct gggtcatgtc catgctctta ccgtgagcca taaacttgtt aatcacatca
    57661 gcaaacaagt ctggtttcac gttggagaaa tattccacct ggttgtattc gctaggcgcg
    57721 gccagttttt cgaacgcagc catgtcagac atggtgttcg gcgactgctt cgcttttgcg
    57781 acccactggt cgaattcggc gcgatccggt gttgcaatag ctttgaactt catgcctgag
    57841 aagcccgggc cgctgtagct ggcggagata ccgtcataag taccgggttc gttggcgatc
    57901 agatgcaggc gagtctgcat accagccatg gcataaatct ggctacccag acgcggaatg
    57961 aagaaggagt tcatcacgga gttggaggtc actttgaagt acaccggagt gttcgccggg
    58021 aaagcgattt cattcacggt agcaatgccc tgttccgggt agatgaagaa ccatttccag
    58081 tccatggaaa ccacttcgat ggtaatgggc ttctcgtcgt gtgccagcgg cttgctaggc
    58141 tcaagagcgt gagtggtttt ccaggtcagt accgcaagga agatgatgat taagataggt
    58201 accgtccaga ccacagcttc cactttattg gagtgtgacc agttcgggct gtacttagca
    58261 tctttattgc tcgcacgata cttccaggcg aaaccaacag ccatcaagat tgcgggaata
    58321 acgacaatca acatcaggcc aaatgccgtc agtatcagtg aacgttgctc cagaccaatc
    58381 tgtcctttgg gatctaacag cgcagaatta cagccactga gcaatacagt gcctgcaaat
    58441 aatgacaacc atcccaaact tttattgtat ttcctgagtc tcatttaacg acctcaattc
    58501 cacgggatct ggtggcgttt aaagtgtgtg ggcattttat gggaaggtta cattactgta
    58561 aacatgatta aattcctgtt acttggattt gactgtcagg tcacatatgt tgcaaaacat
    58621 atcaagttga ttttaagaag ctgttgcaaa agggcgttat aacgaaaggg gagtgcagaa
    58681 ggagtttgga tgggcaattg gtataaccaa tctggaataa aataattgtt taacaaataa
    58741 ttatcattat ggtgacaatt gggtgggggg aaaataaaga ctcgttagct gaatcgtgta
    58801 agaaaaaata gaattcataa attgcgtcaa taatttccaa acgctaagcc gcacaaaaga
    58861 acaaatatta ttaagcgata ctgatgttta tttagccgta ataattacgg cgagtgatta
    58921 ctacagctaa ataatattta cagattacgt cagatgcgtt tttcgtagag ccagataatc
    58981 cagcaaacca ccaagcacga cgccagaaag ggcgactaaa accccgactt ccagcagagc
    59041 aggcaggaaa gagaagtgcg tcaaatccag cgcgtccatc gtcaacagca gtaaccacac
    59101 ggccaacagg ctgacgcccg ccgccagtgt ccacatggca aaggcataac ctgccggata
    59161 ttcggtacgg gagataaagt tgtcatttac tcgtgtatat tcaagcgtct ggcggcaaac
    59221 cagcagcaaa ataagccccg gtacggcagc ggcgacggag aatagataga atgtcgacca
    59281 gccgtgtgct tcaacaaacc aacccgccac ggggccgaca taaactcgtc ctacagcgga
    59341 aagcgctgag agcagggcaa attgagtagc ggaaaatgac ttattacaca gcgtcattaa
    59401 cagcgcgaca aaggctgatg tgcccatccc gccacagagg ttttcgaaaa agacggctgc
    59461 gcccatgctg tagagatgct tatcagtaat cgacagcagc cagtaaccag cgttagacgc
    59521 accttgtaaa atgccgaaaa tcagcagcgc ccggaacagc gacaggcgct gcatcaaaat
    59581 cccaccgtac aatgcgccca caatggtcgc cagtaagcca agcgttttgt taaccacgcc
    59641 tacttcaccc gcatcaaacc cgacgccgcg aatcaaaaac gtggttgtca ggctcatggc
    59701 gaatgcgtcg cccagcttat acagcacgat aagaagcaaa ataagccagg cattattgcg
    59761 accaaagaaa tctcgcagag gtgcaacaac cgcttgttcc agcgtttttg gcacaggaat
    59821 ggtgtcggtc ggttctggtg caagcaacgt cgcgataata caagggatca acagcgccgc
    59881 catcaaccag tacatgccct gccagcccag ccatttatct gccagccaca gggctaagcc
    59941 gccggaaacc agcatcccta aacggtaacc cagcacgctg attgccgcgc ccgcaccacg
    60001 ttcttctgcc gggagcacat cggttttcca cgcatcgaag acaatatcct gggaggccga
    60061 gcaaaaagcg atcaccaccg ccagtgccgc catccagcgg agttgggtgc caggttcgag
    60121 aaaacccatc gccgcaatgg cgactaataa caggatttgc gtggcgagca gccaaccgcg
    60181 ccgccgccca aaaaatgggg gcgtgtagcg gtccatcagc ggtgaccaga ggaatttaaa
    60241 aacgtaagcc tggcctacca gagagaagaa accaatggtt ttgagatcaa tattctcgac
    60301 cgtcatccag gcctgtaagg taccggatgt cagggcgagc ggtagcccgg aagcaaaacc
    60361 caggatcagc aatatggctg aacgcggctg ttgaaaaata cgtaaatatt gactggacat
    60421 gggcgtctac aggcccggtt tgcaccgggc cagagggcag aaattaacgc gcgttctgct
    60481 tgatgaattc gtgaatgctg gtgtcctgag acatatcagc gatggtatcg gtcagcacac
    60541 tgttaaccgc atcggcgata tttttgttgg aggcctggaa cgcaccttca acgttgtagc
    60601 tggcacgata gtttttggtt attttgttgc cattctgcgc ggtagcgatg atggcgatat
    60661 ccgctttggt agcgatgttg tagcgcacgt tgccctggga cacgtcagca tacagttggc
    60721 taacgatgat ttgcagatta accgggccat tcggaccaac catgtaacca cgcgcggtca
    60781 tctgtttttc cagcacttct tgcagcagga aacgcagatc gcgggaggcg gccagggtaa
    60841 cgatttgatt atcgcgggtg acttttgcca gcgcctgatc ggtacgctga tcggcaccat
    60901 taatgcttac ggtgacgccc atcaggcttg gatcctgctg tggcagtgta atcgtcgggg
    60961 aaacttcaat agttgttggc ggttttgcgc atcctgcaag cataaacaga gcaactaacg
    61021 ggaagaggat ttttttgaac atgttcgggc tctcagagac tcttaagcgt gtttggtaaa
    61081 aattcccgcc atcataacat tgccaacggc gaggggaagt gggtaaggca tgtaaattca
    61141 tcatgttgac gaaataatca cccctggtaa aagaaacgct gatgcgaggc ctgtgtttca
    61201 atctttaaat cagtaaactt catacgcttg acggaaaaac caggacgaaa cctaaatatt
    61261 tgttgttaag ctgcaatgga aacggtaaaa gcggctagta tttaaaggga tggatgacat
    61321 ctcagcgttg tcggaggaga tatttcatga tgatacgtga gcggatagaa gaaaaattaa
    61381 gggcggcgtt ccaacccgta ttcctcgaag tagtggatga aagctatcgt cacaatgtcc
    61441 cagccggctc tgaaagccat tttaaagttg tgctggtcag cgatcgtttt acgggtgaac
    61501 gttttctgaa tcgtcatcga atgatttaca gtactttagc ggaggaactc tctactaccg
    61561 ttcatgcgct ggctctgcat acttacacta ttaaggagtg ggaagggttg caggacaccg
    61621 tctttgcctc tcctccctgt cgtggagcag gaagcatcgc gtaaaaacgc atttgcaact
    61681 gtcggcgctt tttcagtatg ttgctaaaga ttttatgaaa aacggcctgc gggccgtttt
    61741 gttttgtctg gattttgcgc tttttgccca gcattcagac gaaaattgcc cgggaattgt
    61801 gaaaaaatac gcgacagcgc gcaataaccg ttctcgactc ataaaagtga tgccgctata
    61861 atgccgcgtc ctatttgaat gctttcggga tgattctggt aacagggaat gtgattgatt
    61921 ataagaacat cccggttccg cgaagccaac aacctgtgct tgcggggtaa gagttgaccg
    61981 agcactgtga ttttttgagg taacaagatg caagtttcag ttgaaaccac tcaaggcctt
    62041 ggccgccgtg taacgattac tatcgctgct gacagcatcg agaccgctgt taaaagcgag
    62101 ctggtcaacg ttgcgaaaaa agtacgtatt gacggcttcc gcaaaggcaa agtgccaatg
    62161 aatatcgttg ctcagcgtta tggcgcgtct gtacgccagg acgttctggg tgacctgatg
    62221 agccgtaact tcattgacgc catcattaaa gaaaaaatca atccggctgg cgcaccgact
    62281 tatgttccgg gcgaatacaa gctgggtgaa gacttcactt actctgtaga gtttgaagtt
    62341 tatccggaag ttgaactgca gggtctggaa gcgatcgaag ttgaaaaacc gatcgttgaa
    62401 gtgaccgacg ctgacgttga cggcatgctg gatactctgc gtaaacagca ggcgacctgg
    62461 aaagaaaaag acagcgctgt tgaagcagaa gaccgcgtaa ccatcgactt caccggttct
    62521 gtagacagcg aagagttcga aggcggtaaa gcgtctgatt tcgtactggc gatgggccag
    62581 ggtcgtatga tcccgggctt tgaagacggt atcaaaggcc acaaagctgg cgaagagttc
    62641 accatcgacg tgaccttccc ggaagaatac cacgcagaaa acctgaaagg taaagcagcg
    62701 aaattcgcta tcaacctgaa gaaagttgaa gagcgtgaac tgccggaact gactgcagaa
    62761 ttcatcaaac gtttcggcgt tgaagatggt tccgtagaag gtctgcgcgc tgaagtgcgt
    62821 aaaaacatgg agcgcgagct gaagagcgcc atccgtaacc gcgttaagtc tcaggcgatc
    62881 gaaggtctgg taaaagctaa cgacatcgac gtaccggctg cgctgatcga cagcgaaatc
    62941 gacgttctgc gtcgccaggc tgcacagcgt ttcggtggca acgaaaaaca agctctggaa
    63001 ctgccgcgcg aactgttcga agaacaggct aaacgccgcg tagttgttgg cctgctgctg
    63061 ggcgaagtta tccgcaccaa cgagctgaaa gctgacgaag agcgcgtgaa aggcctgatc
    63121 gaagagatgg cttctgcgta cgaagatccg aaagaagtta tcgagttcta cagcaaaaac
    63181 aaagaactga tggacaacat gcgcaatgtt gctctggaag aacaggctgt tgaagctgta
    63241 ctggcgaaag cgaaagtgac tgaaaaagaa accactttca acgagctgat gaaccagcag
    63301 gcgtaattta cgcagcataa cgcgctaaat tcgcacaaag gcccgtcacc gccaggtggt
    63361 gggctttttt tgtcatgaat tttgcatgga accgtgcgaa aagcctcttt cggtgttagc
    63421 gtaacaacaa aagattgtta tgcttgaaat atggtgatgc cgtacccata acacagggac
    63481 tagctgataa tccgtccata aggttacaat cggtacagca ggttttttca attttatcca
    63541 ggagacggaa atgtcataca gcggcgaacg agataacttt gcaccccata tggcgctggt
    63601 gccgatggtc attgaacaga cctcacgcgg tgagcgctct tttgatatct attctcgtct
    63661 acttaaggaa cgcgtcattt ttctgactgg ccaggttgaa gaccacatgg ctaacctgat
    63721 tgtggcgcag atgctgttcc tggaagcgga aaacccagaa aaagatatct atctgtacat
    63781 taactcccca ggcggggtga tcactgccgg gatgtctatc tatgacacca tgcagtttat
    63841 caagcctgat gtcagcacca tctgtatggg ccaggcggcc tcgatgggcg ctttcttgct
    63901 gaccgcaggg gcaaaaggta aacgtttttg cctgccgaat tcgcgcgtga tgattcacca
    63961 accgttgggc ggctaccagg gccaggcgac cgatatcgaa attcatgccc gtgaaattct
    64021 gaaagttaaa gggcgcatga atgaacttat ggcgcttcat acgggtcaat cattagaaca
    64081 gattgaacgt gataccgagc gcgatcgctt cctttccgcc cctgaagcgg tggaatacgg
    64141 tctggtcgat tcgattctga cccatcgtaa ttgatgccag aggcgcaact gtgccgctat
    64201 acttatccag ggcggcacaa cgctgtaagc ggcttgcgcc tgagaatggc atttgcgtcg
    64261 tcgtgtgcgg cacaaagaac aaagaagagg ttttgaccca tgacagataa acgcaaagat
    64321 ggctcaggca aattgctgta ttgctctttt tgcggcaaaa gccagcatga agtgcgcaag
    64381 ctgattgccg gtccatccgt gtatatctgc gacgaatgtg ttgatttatg taacgacatc
    64441 attcgcgaag agattaaaga agttgcaccg catcgtgaac gcagtgcgct accgacgccg
    64501 catgaaattc gcaaccacct ggacgattac gttatcggcc aggaacaggc gaaaaaagtg
    64561 ctggcggtcg cggtatacaa ccattacaaa cgtctgcgca acggcgatac cagcaatggt
    64621 gtcgagttgg gcaaaagtaa cattctgctg atcggtccga ccggttccgg taaaacgctg
    64681 ctggctgaaa cgctggcgcg cctgctggat gttccgttca ccatggccga cgcgactaca
    64741 ctgaccgaag ccggttatgt gggtgaagac gttgaaaaca tcattcagaa gctgttgcag
    64801 aaatgcgact acgatgtcca gaaagcacag cgtggtattg tctacatcga tgaaatcgac
    64861 aagatttctc gtaagtcgga caacccgtct attacccgtg acgtttccgg tgaaggcgta
    64921 cagcaggcac tgttgaaact gatcgaaggt actgttgctg ccgttccacc gcagggcggg
    64981 cgtaagcatc cgcagcagga atttttgcag gttgatacct ccaagatcct gtttatttgt
    65041 ggcggtgcgt ttgccggtct ggataaagtg atttcccacc gtgtagaaac cggctccggc
    65101 attggttttg gcgcgacggt aaaagcgaag tccgacaaag caagcgaagg cgagctgctg
    65161 gcgcaggttg aaccggaaga tctgatcaag tttggtctta tccctgagtt tattggtcgt
    65221 ctgccggttg tcgcaacgtt gaatgaactg agcgaagaag ctctgattca gatcctcaaa
    65281 gagccgaaaa acgccctgac caagcagtat caggcgctgt ttaatctgga aggcgtggat
    65341 ctggaattcc gtgacgaggc gctggatgct atcgctaaga aagcgatggc gcgtaaaacc
    65401 ggtgcccgtg gcctgcgttc catcgtagaa gccgcactgc tcgataccat gtacgatctg
    65461 ccgtccatgg aagatgtcga aaaagtggtt atcgacgagt cggtaattga tggtcaaagc
    65521 aaaccgttgc tgatttatgg taagccggaa gcgcaacagg catctggtga ataattaacc
    65581 attcccatac aattagttaa ccaaaaaggg gggattttat ctccccttta atttttcctc
    65641 tattctcggc gttgaatgtg ggggaaacat ccccatatac tgacgtacat gttaatagat
    65701 ggcgtgaagc acagtcgtgt catctgatta cctggcggaa attaaactaa gagagagctc
    65761 tatgaatcct gagcgttctg aacgcattga aatccccgta ttgccgctgc gcgatgtggt
    65821 ggtttatccg cacatggtca ttcccttatt tgtcgggcgg gaaaaatcta tccgttgtct
    65881 ggaagcggcg atggaccatg ataaaaaaat tatgctggtc gcgcagaaag aagcttcaac
    65941 ggatgagccg ggtgtaaacg atcttttcac cgtcgggacc gtggcctcta tattgcagat
    66001 gctgaaactg cctgacggca ccgtcaaagt gctggtcgag gggttacagc gcgcgcgtat
    66061 ttctgcgctc tctgacaatg gcgaacactt ttctgcgaag gcggagtatc tggagtcgcc
    66121 gaccattgat gagcgggaac aggaagtgct ggtgcgtact gcaatcagcc agttcgaagg
    66181 ctacatcaag ctgaacaaaa aaatcccacc agaagtgctg acgtcgctga atagcatcga
    66241 cgatccggcg cgtctggcgg ataccattgc tgcacatatg ccactgaaac tggctgacaa
    66301 acagtccgtt ctggagatgt ccgacgttaa cgaacgtctg gaatatctga tggcaatgat
    66361 ggaatcggaa atcgatctgc tgcaggttga gaaacgcatt cgcaaccgcg ttaaaaagca
    66421 gatggagaaa tcccagcgtg agtactatct gaacgagcaa atgaaagcta ttcagaaaga
    66481 actcggtgaa atggacgacg cgccggacga aaacgaagcc ctgaagcgca aaatcgacgc
    66541 ggcgaagatg ccgaaagagg caaaagagaa agcggaagca gagttgcaga agctgaaaat
    66601 gatgtctccg atgtcggcag aagcgaccgt agtgcgtggt tatatcgact ggatggtaca
    66661 ggtaccgtgg aatgcgcgca gcaaggtcaa aaaagacctg cgtcaggcgc aggaaatcct
    66721 tgataccgac cattatggtc tggagcgcgt gaaagatcgc atccttgagt accttgcggt
    66781 tcaaagccgt gtcaacaaaa tcaagggacc gatcctctgc ctggtagggc cgccgggggt
    66841 aggtaaaacc tctcttggtc agtccattgc caaagccacc gggcgtaaat atgtccgtat
    66901 ggcgctgggc ggcgtgcgtg atgaagcgga aatccgtggt caccgccgta cttacatcgg
    66961 ttctatgccg ggtaaactga tccagaaaat ggcgaaagtg ggcgtgaaaa acccgctgtt
    67021 cctgctcgat gagatcgaca aaatgtcttc tgacatgcga ggcgatccgg cctctgcact
    67081 gcttgaagtg ctggatccag agcagaacgt agcgttcagc gaccactacc tggaagtgga
    67141 ttacgatctc agcgacgtga tgtttgtcgc gacgtcgaac tccatgaaca ttccggcacc
    67201 gctgctggat cgtatggaag tgattcgcct ctccggttat accgaagatg aaaaactgaa
    67261 catcgccaaa cgtcacctgc tgccgaagca gattgaacgt aatgcactga aaaaaggtga
    67321 gctgaccgtc gacgatagcg ccattatcgg cattattcgt tactacaccc gtgaggcggg
    67381 cgtgcgtggt ctggagcgtg aaatctccaa actgtgccgc aaagcggtta agcagttact
    67441 gctcgataag tcattaaaac atatcgaaat taacggcgat aacctgcatg actacctcgg
    67501 tgttcagcgt ttcgactatg gtcgcgctga taacgaaaac tgtgtcggtc aggtaaccgg
    67561 tctggcgtgg acggaagtgg gcggtgactt gctgaccatt gaaaccgcat gtgttccggg
    67621 taaaggcaaa ctgacctata ccggttcgct cggcgaagtg atgcaggagt ctattcaggc
    67681 ggcgttaacg gtggttcgtg cgcgtgcgga aaaactgggg atcaaccctg atttttacga
    67741 aaaacgtgac atccacgtcc acgtaccgga aggtgcgacg ccgaaagatg gtccgagtgc
    67801 cggtattgct atgtgcaccg cgctggtttc ttgcctgacc ggtaacccgg ttcgtgccga
    67861 tgtggcaatg accggtgaga tcactctgcg tggtcaggta ctgccgatcg gtggtttgaa
    67921 agaaaaactc ctggcagcgc atcgcggcgg gattaaaaca gtgctaattc cgttcgaaaa
    67981 taaacgcgat ctggaagaga ttcctgacaa cgtaattgcc gatctggaca ttcatcctgt
    68041 gaagcgcatt gaggaagttc tgactctggc gctgcaaaat gaaccgtctg gcatgcaggt
    68101 tgtgactgca aaatagtgac ctcgcgcaaa atgcactaat aaaaacaggg ctggcaggct
    68161 aattcgggct tgccagcctt tttttgtctc gctaagttag atggcggatc gggcttgccc
    68221 ttattaaggg gtgttgtaag gggatggctg gcctgatata actgctgcgc gttcgtacct
    68281 tgaaggattc aagtgcgata taaattataa agaggaagag aagagtgaat aaatctcaat
    68341 tgatcgacaa gattgctgca ggggctgata tctctaaagc tgcggctggc cgtgcgttag
    68401 atgctattat tgcttccgta actgaatctc tgaaagaagg ggatgatgta gcactggtag
    68461 gttttggtac ttttgccgtt aaagagcgtg ctgcccgtac tggccgcaac ccgcagaccg
    68521 gtaaagagat caccatcgct gctgctaaag taccgagctt ccgtgcaggt aaagcactga
    68581 aagacgcggt aaactaagcg ttgtccccgg tggggatgtg acgaagttca agggcgcatc
    68641 tactgatgtg ccttttttat ttgtattcgg tgactttctg cgtcttgtgg gctgacaatt
    68701 gcccccgttt cttgtcacaa taggcctttg cgcgcatcga tacgttgcgt gaggtacaca
    68761 gtcatctaca gcggagtgtt gttacaccat gatggacagc ttacgcacgg ctgcaaacag
    68821 tctcgtgctc aagattattt tcggtatcat tatcgtgtcg ttcatattga ccggcgtgag
    68881 tggttacctg attggcggag gcaataacta cgccgcaaaa gtgaatgacc aggaaatcag
    68941 ccgtgggcag ttcgagaacg cctttaacag cgagcgtaat cgcatgcagc aacagctggg
    69001 cgatcaatac tccgagctgg cagcgaatga aggctatatg aaaaccctgc gtcaacaggt
    69061 gctgaatcgt ctgatcgacg aggcgctgtt ggatcagtac gctcgtgagc tgaaactggg
    69121 tatcagtgat gagcaggtta aacaggcgat tttcgcgacc ccagccttcc aggttgacgg
    69181 caaatttgat aacagccgct ataacggtat cctcaaccag atggggatga ccgccgatca
    69241 gtacgcccag gcgctgcgta accagctcac tacccaacag ctgattaacg gtgttgccgg
    69301 taccgatttt atgctgaaag gtgaaaccga cgagctggcg gcactggtcg cgcaacaacg
    69361 cgtggtgcgt gaggcgacta tcgatgttaa cgcgctggcg gcgaagcagc ctgtgaccga
    69421 acaggagatt gccagctact acgaacaaaa caaaaacaat ttcatgacgc cggaacaatt
    69481 ccgcgtgagt tacatcaagc tggatgccgc aacgatgcag caaccggtta gcgatgcgga
    69541 tatccagagc tactacgatc agcatcagga tcaattcacc cagccgcagc gtacccgcta
    69601 cagcatcatc cagaccaaaa ctgaagatga agcgaaagcg gtacttgatg agctgaataa
    69661 aggcggtgat tttgctgcat tagccaaaga aaaatctgcc gatattatct ctgcccgtaa
    69721 cggcggcgat atgggttggt tagaagatgc cactatcccc gacgaactga aaaatgctgg
    69781 tctgaaagaa aaaggccaac tgtctggtgt catcaaatct tcggtcggtt tcctgattgt
    69841 acgtctggac gacattcagc cagcgaaagt gaaatcgtta gacgaagtac gtgacgacat
    69901 tgcggcgaaa gtgaaacacg aaaaagccct cgatgcgtac tatgcgctgc agcagaaagt
    69961 gagcgatgcg gcaagcaacg acaccgagtc tctggccggt gcagagcaag ctgccggcgt
    70021 taaagccact cagacgggtt ggttcagcaa agataacctg ccggaagagt tgaacttcaa
    70081 gccggttgcc gacgctatct ttaacggcgg tctggtaggt gaaaacggcg cgccgggcat
    70141 caactctgac atcatcaccg tagacggcga ccgtgcattc gtgctgcgca tcagcgagca
    70201 caaaccggaa gcggtgaaac cgttggcaga tgttcaggaa caagttaagg cactggttca
    70261 gcacaacaaa gctgaacaac aggcgaaagt ggatgctgag aaactgctgg ttgatttgaa
    70321 agccggcaaa ggtgcggaag ctatgcaggc tgccggtctg aaatttggcg agccgaaaac
    70381 cttaagccgt tccggtcgtg acccgattag ccaggcggcg tttgcactgc cactgccagc
    70441 gaaagacaaa ccgagctacg gtatggcgac cgatatgcaa ggtaatgtgg ttctgctggc
    70501 gctggatgaa gtgaaacaag gttcaatgcc ggaagatcag aaaaaagcga tggtgcaggg
    70561 tatcacccag aacaacgcac aaatcgtctt tgaagctctg atgagtaacc tgcgtaaaga
    70621 ggcgaaaatc aaaattggcg atgcgctgga acagcaataa tcctgaagcc gcctcgcaaa
    70681 aaaatgcgtt gcagctgtaa caagaaaagg tcgctttcgc ggccttttcc atttctgaac
    70741 attgccattt gtttactgtt ttcactgccg ttaaggtgat tccactgtta acaaacaagg
    70801 agaaaacagt atgaaacacg gaattaaagc actgctcatt accctgtccc tggcctgtgc
    70861 cggaatgtct catagcgcgc tggcggcagc ttctgtggcg aaaccgacgg cggtagaaac
    70921 caaagcggaa gctcctgcag cacaaagtaa agcagcagta ccggcgaaag ccagtgacga
    70981 agaaggcacc cgggtcagca ttaataatgc cagcgcggaa gagctagccc gcgcgatgaa
    71041 tggcgttggc ctgaagaaag cgcaggcgat tgtcagttat cgcgaagagt acggtccgtt
    71101 taaaacagtg gaggatctaa agcaggtgcc ggggatgggc aattcgctgg tggaacgtaa
    71161 tctggcggta ttaaccctgt aattaatttg catagtggca attttttgcc agactgaaga
    71221 ggtcatacca gttatgacct ctgtacttat aacaacaacg taaggttatt gcgctatgca
    71281 aacacaaata aaagttcgtg gatatcatct cgacgtttac cagcacgtca acaacgcccg
    71341 ctaccttgaa tttctcgaag aagcccgctg ggatgggttg gaaaatagcg acagttttca
    71401 gtggatgacg gcccataaca tcgccttcgt cgtggtcaat atcaatatta actatcgtcg
    71461 cccagcggta ttaagtgacc tgttaactat taccagtcag ttgcagcaat taaacggtaa
    71521 aagcggcatc ttaagccagg tcattacact ggagccggaa gggcaggtgg tagcggatgc
    71581 gcttattacg tttgtttgta ttgatcttaa aacgcagaaa gcattagctc tggaagggga
    71641 attgcgcgaa aagctggagc agatggttaa gtaaacgttt tgtggtgccg gatgctcaag
    71701 ccgcatccgg cgacacccgg aataattacc tcaacccggt tttctgcttc atcgctgcca
    71761 tcaccgtcgg tttatcggcc agataatgat tcaaaccgtt ggcgcgtaaa ttacatgccg
    71821 cacaatgacc gcaaccgtcg cctttaatgc cgttatagca ggtcaacgtt tcgttacgga
    71881 ctaaatccag tttgccgtaa taatctgcca gcgcccaggt ttccgcttta tcaatccaca
    71941 tcagcggcgt ttcaaaacga atatctttcg ccatgcccaa actgacggca tggtttagtg
    72001 ctttcacaaa ctcatcgcgg caatccgggt agccggagaa atccgtttca cagacgccgg
    72061 taattacggc ttctgctttt acctgatacg catatattgc cgccagcgtc aggaacaaaa
    72121 tattacgccc tgggacaaac gtattcggga tgccatcggc ttcaggttca taatcaggca
    72181 ccggaatgct gtcacgtgtc aggctgctga ccgccagctc gttgagcagc gttacatcaa
    72241 gaaccttatg cgcgcgtgct cccagtttca gcgccagttc gcgtgccacg tcgatttctg
    72301 cgcgatgtcg ctgaccgtaa tcgaacgtca cgcaatggac ttcatcatat tgttgtaatg
    72361 cctgcaccag acaggtggtg gaatcctggc ctccactgaa cacaacgaca gcacgtttca
    72421 tagataatcc tgcttgaaca ataaaagcgt tatggtaacg cctgcgatta acccggacca
    72481 gctattcatt gcgacggcgc gggaagccag gcttcggtaa attcaaacca gccgcgcggt
    72541 gtcagtcgca caccgttcac gccgggaggg gcactaatgc gatagtgata gttgaacagc
    72601 ggcgtcagcg tcgcgtctgc cattaactgg ctaaaaaccg ctttcagggc attaaatcgg
    72661 ttttcctcat caggcattac ttgcaccgcg tccagcgtcg attgcagatg tgcatatgct
    72721 ggagcgtcga aaacatgtgg ccatagtgga tcgcaacgta gccattgctc cagagtatat
    72781 tccggtgctt cgccaattaa tctgtcgccc atcatgaggt ctgcgtgtgc cagtagggtc
    72841 gtgtcgtccc agttttttgc gttatgaaaa ataattgtga gttcacagcc ttccgctgcc
    72901 agtgtcgcct gtaggcgttc tgccatggta tgaagttcta tcggtaggtg ataaaccagc
    72961 gtcaaggttt tcggtagttt gacttcatcc ggtacttgcc agtgcggaat agtccagcct
    73021 ggcagtaatg catgactggc ggtgatcagg ttttctccga cttctaacgt ttgtaataaa
    73081 ccggattgat gaataatgga gatcactttt cgcgcctgcc agagggagag tcggggactt
    73141 ttgcgcaacg ttaaatagca aaaacctaaa ctgatgccac tactgacctg gctgacccgt
    73201 tgcagctcct ccggtttgcc gatggtgatt tgcacgggat gccgacaact ggttcccaaa
    73261 tctttttcga aaagcggcgg agttatccag tactcaaccg ctttaagcag cggatgacgc
    73321 aaatggtaat aatcatggct ctccaggcgc accagctccg ctgtgaattg tgttaagcga
    73381 aaaggaccag tgccgatcag tgggaattgc ggatgcgcca gatggctgca atagctcgcc
    73441 agccggtgcg caagccagta atcggggcga tgtaaaaaga aggtcagaca ctgcggatgg
    73501 gtgacttcaa tacgcttcac gctaataaat aattgatcca gtgctggcag ttgtaacagc
    73561 atcaataatc gctggtgtaa gtgtgaggct tttactgcat cgccgttatg ccagtgtagg
    73621 gttgaacgaa gataaaagtc ccagcgtaac ccgtcagtag aggtttccca gtgatgcgct
    73681 aaatcgccaa tcgggcgctg agtattatta tcgaagcggg tcaggccgga aaatatctgc
    73741 ccggcgagat gctgctcggc acggccgggc aaaaagcctg gttgtagcgg ttcgagcggg
    73801 cgatagtagg gaatacgcaa cgtgggtgta tcgttttgcc attgtccgcc cataaacggc
    73861 tgtaacagag tgcgtagctc acctggggcc agttgcgcca gctccagcac atcttgctgc
    73921 tttccggttt ccagtgcctg ttccatcatc gcattgcgta gcgattccgg cgtgaccaga
    73981 aagcgtaatt gtccgcgctt tccgcgtcct gactgcgcct gccactccag ccatcccgcc
    74041 tcctgtgcct gacgcaacag cgtacgaaca tggcgttcgc tgcaaaaaca gcgttcggcc
    74101 agttcgctga cggtgacggt ttgcggcttt ccggctgaag gttgccacag acgttgatac
    74161 tggttaagac ggttgagcaa tcgcatataa acccggaaca atattattta actattcact
    74221 attacttccg tatatatcag gtgatactca atctccatta accgtgtcac agagtggaga
    74281 aagaaatggc tcgtctggca gcatttgata tggatggcac tttattgatg cctgaccatc
    74341 atttaggtga gaaaaccctc tctactttgg cgcgactgcg tgaacgcgac attaccctca
    74401 cttttgccac ggggcgtcat gcgctggaga tgcagcatat tctcggggcg ctatcgctgg
    74461 atgcatattt gattaccggc aacggaacgc gcgtgcattc tctggaaggt gaacttttac
    74521 atcgtgatga tttacctgcg gatgtcgcgg agctggtgct gtatcagcaa tgggataccc
    74581 gagccagcat gcatatcttc aatgacgacg gttggtttac cgggaaagag atccctgcgt
    74641 tgttgcaggc atttgtctat agcggttttc gttatcagat aatcgatgtc aaaaaaatgc
    74701 cactcggcag cgtcaccaag atctgcttct gtggcgatca cgacgatctt acacgcttgc
    74761 agatccagct atacgaagca ttaggcgagc gtgcacattt gtgtttttcc gccacggatt
    74821 gcctcgaagt gctgccggtg ggctgcaata aaggcgctgc attgacggtg ctgacccaac
    74881 atctaggttt atcgttgcgc gattgcatgg cctttggtga tgcgatgaac gatcgcgaaa
    74941 tgttaggcag cgtcggtagc gggtttatta tgggcaatgc gatgccgcaa ctgcgcgcgg
    75001 agctcccgca tttaccggtg attggacatt gccgaaatca ggctgtctct cactatttga
    75061 cgcactgact ggactatcca catctacctt attcccccga ataatgagat cccttccagc
    75121 accgggcaat tgcccggttt tttttgcgtt gaatttgtca ttttgtgccg tggtgtttaa
    75181 accgcacaga ataaattgtc gtgatttcac ctttaaaata gaattaaaag agaaaaaaat
    75241 tctctgtgga agggctatgt tagataaaat tgaccgtaag ctgctggcct tactgcagca
    75301 ggattgcacc ctctctttgc aggcactggc tgaagccgtt aatctgacaa ccaccccttg
    75361 ctggaagcgc ctgaaacggc tggaggacga cggtatcctt atcggcaaag tcgccctgct
    75421 ggatccggaa aaaataggcc tcggcctgac cgcctttgtg ctgataaaaa cgcaacatca
    75481 cagtagcgaa tggtattgcc gctttgtcac ggtggttacc gaaatgccag aagtgctggg
    75541 gttctggcgc atggctggtg aatacgatta tctgatgcgc gtccaggttg ccgacatgaa
    75601 acgctacgac gagttttata agcgtctggt aaacagcgtg ccggggctgt cggacgtcac
    75661 ttccagcttc gcgatggaac agattaaata caccacttct ttacccatcg aataaatatc
    75721 cagaatcagg tcaggacaca acgcgtgcga ttatttgctc aattaagctg gtatttccgt
    75781 cgggaatggc gtcgctatct cggggctgtc gccttgcttg tcattatcgc gatgctgcaa
    75841 ctggttccgc caaaagtggt tggtattgtt gtcgatggcg tgacagaaca acactttact
    75901 accgggcaga tcctgatgtg gatcgccacc atggtgctga ttgccgttgt ggtttatttc
    75961 ctgcgttacg tctggcgggt attgctgttt ggtgcgtctt atcaactggc tgttgaactg
    76021 cgtgaagatt attaccgtca gctaagccgg cagcatcctg agttttacct gcgtcatcgc
    76081 acgggtgacc tcatggctcg tgcgaccaat gacgtcgatc gcgtcgtgtt tgcagccgga
    76141 gaaggggtgc tgacgctggt ggattcactg gtgatgggct gcgctgtgtt gattatgatg
    76201 tctacgcaaa ttagctggca gttgacctta tttgccctgt tgccgatgcc agtgatggcg
    76261 atcatgatca agcgcaacgg cgatgccttg catgaacgct ttaagctggc acaggcggcg
    76321 ttttctagtc ttaatgaccg cacccaggaa agcctcacca gtatccgcat gatcaaagcc
    76381 tttggtctgg aagatcgcca gtcggcgtta tttgccgcgg atgccgaaga taccggcaaa
    76441 aaaaacatgc gggtggcgcg tattgatgct cgtttcgacc cgaccatcta tatcgcgatt
    76501 ggtatggcga acttgctggc gattggcggc ggtagttgga tggtggtgca gggcagttta
    76561 acgctgggcc agctcaccag ttttatgatg tatttaggtc tgatgatttg gccaatgctg
    76621 gcgctggcat ggatgtttaa cattgtggaa cgtggtagtg ctgcatacag ccgtattcgc
    76681 gcgatgctgg cggaagcgcc ggtggtgaac gatggtagcg aacccgtgcc ggaagggcgt
    76741 ggcgaactgg atgtaaatat tcaccagttc acgtatccgc agactgacca tcctgcgctg
    76801 gaaaacgtca atttcgccct gaaacccggt cagatgctgg gtatctgcgg gccgactggt
    76861 tccggcaaaa gtaccctgtt gtcgctcatt cagcgtcatt tcgacgtcag cgagggggat
    76921 attcgctttc atgatattcc tctgacgaag ttacaactcg atagctggcg tagccgcctg
    76981 gcggtagtta gccagacacc gttccttttt tccgacaccg tagcgaacaa catcgcgttg
    77041 ggttgcccga atgccaccca gcaagagatt gagcatgtcg cgcggttagc cagcgtacat
    77101 gacgatattt tgcgtctacc gcaaggttac gatacagaag tgggcgagcg cggcgtgatg
    77161 ctttccggtg ggcaaaaaca gcgtatctcc attgctcgag cgttattagt caacgcggaa
    77221 atcctcatcc ttgatgatgc gctttcggcg gtggacggac gcacagagca ccagatcctg
    77281 cataacctgc gtcagtgggg acagggaaga acggtaatca tcagtgccca tcgcctttcg
    77341 gcactgacgg aagccagtga aaatgtaatg aacgcatc
//
LOCUS       DAZPEB010000003        76634 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZPEB010000003 DAZPEB010000000
VERSION     DAZPEB010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 76634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76634
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.003"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            414..1616
                     /gene="proV"
                     /locus_tag="ACHURJ_000150"
     CDS             414..1616
                     /gene="proV"
                     /locus_tag="ACHURJ_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFR1279252.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            1609..2673
                     /gene="proW"
                     /locus_tag="ACHURJ_000151"
     CDS             1609..2673
                     /gene="proW"
                     /locus_tag="ACHURJ_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFR1279253.1"
                     /translation="MADQNNPWDTTPAADSAVQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPSIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            2731..3723
                     /gene="proX"
                     /locus_tag="ACHURJ_000152"
     CDS             2731..3723
                     /gene="proX"
                     /locus_tag="ACHURJ_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFR1279254.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTLLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKEPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            4015..5199
                     /locus_tag="ACHURJ_000153"
     CDS             4015..5199
                     /locus_tag="ACHURJ_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1279255.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            5323..6060
                     /gene="ygaZ"
                     /locus_tag="ACHURJ_000154"
     CDS             5323..6060
                     /gene="ygaZ"
                     /locus_tag="ACHURJ_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFR1279256.1"
                     /translation="MESPTPQPALGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            6050..6385
                     /gene="ygaH"
                     /locus_tag="ACHURJ_000155"
     CDS             6050..6385
                     /gene="ygaH"
                     /locus_tag="ACHURJ_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFR1279257.1"
                     /translation="MSYEVLLLGLLVGVANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            6476..7006
                     /gene="emrR"
                     /locus_tag="ACHURJ_000156"
     CDS             6476..7006
                     /gene="emrR"
                     /locus_tag="ACHURJ_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFR1279258.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            7133..8305
                     /gene="emrA"
                     /locus_tag="ACHURJ_000157"
     CDS             7133..8305
                     /gene="emrA"
                     /locus_tag="ACHURJ_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFR1279259.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            8322..9860
                     /gene="emrB"
                     /locus_tag="ACHURJ_000158"
     CDS             8322..9860
                     /gene="emrB"
                     /locus_tag="ACHURJ_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFR1279260.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWECGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(9924..10439)
                     /gene="luxS"
                     /locus_tag="ACHURJ_000159"
     CDS             complement(9924..10439)
                     /gene="luxS"
                     /locus_tag="ACHURJ_000159"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFR1279261.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGSHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(10589..12145)
                     /gene="gshA"
                     /locus_tag="ACHURJ_000160"
     CDS             complement(10589..12145)
                     /gene="gshA"
                     /locus_tag="ACHURJ_000160"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFR1279262.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTQATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCCDISGTDAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGKAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(12218..12646)
                     /locus_tag="ACHURJ_000161"
     CDS             complement(12218..12646)
                     /locus_tag="ACHURJ_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFR1279263.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISYPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(12643..13198)
                     /gene="yqaB"
                     /locus_tag="ACHURJ_000162"
                     /pseudo
     CDS             complement(12643..13198)
                     /gene="yqaB"
                     /locus_tag="ACHURJ_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
     gene            complement(13346..13422)
                     /locus_tag="ACHURJ_000163"
     tRNA            complement(13346..13422)
                     /locus_tag="ACHURJ_000163"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13386..13388),aa:Arg,seq:acg)
     gene            complement(13621..13697)
                     /locus_tag="ACHURJ_000164"
     tRNA            complement(13621..13697)
                     /locus_tag="ACHURJ_000164"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13661..13663),aa:Arg,seq:acg)
     gene            complement(13895..13971)
                     /locus_tag="ACHURJ_000165"
     tRNA            complement(13895..13971)
                     /locus_tag="ACHURJ_000165"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13935..13937),aa:Arg,seq:acg)
     gene            complement(13975..14067)
                     /locus_tag="ACHURJ_000166"
     tRNA            complement(13975..14067)
                     /locus_tag="ACHURJ_000166"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14031..14033),aa:Ser,seq:gct)
     gene            complement(14383..14568)
                     /gene="csrA"
                     /locus_tag="ACHURJ_000167"
     CDS             complement(14383..14568)
                     /gene="csrA"
                     /locus_tag="ACHURJ_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFR1279264.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(14803..17433)
                     /gene="alaS"
                     /locus_tag="ACHURJ_000168"
     CDS             complement(14803..17433)
                     /gene="alaS"
                     /locus_tag="ACHURJ_000168"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFR1279265.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKDANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(17561..18061)
                     /gene="recX"
                     /locus_tag="ACHURJ_000169"
     CDS             complement(17561..18061)
                     /gene="recX"
                     /locus_tag="ACHURJ_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFR1279266.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYDEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(18304..19365)
                     /gene="recA"
                     /locus_tag="ACHURJ_000170"
     CDS             complement(18304..19365)
                     /gene="recA"
                     /locus_tag="ACHURJ_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFR1279267.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(19445..19942)
                     /gene="pncC"
                     /locus_tag="ACHURJ_000171"
     CDS             complement(19445..19942)
                     /gene="pncC"
                     /locus_tag="ACHURJ_000171"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFR1279268.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMVIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(20087..21172)
                     /gene="mltB"
                     /locus_tag="ACHURJ_000172"
     CDS             complement(20087..21172)
                     /gene="mltB"
                     /locus_tag="ACHURJ_000172"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFR1279269.1"
                     /translation="MFKRRYVTLFPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            21428..21991
                     /gene="srlA"
                     /locus_tag="ACHURJ_000173"
                     /gene_synonym="gutA"
     CDS             21428..21991
                     /gene="srlA"
                     /locus_tag="ACHURJ_000173"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFR1279270.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSQGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            21988..22947
                     /gene="srlE"
                     /locus_tag="ACHURJ_000174"
                     /gene_synonym="gutE"
     CDS             21988..22947
                     /gene="srlE"
                     /locus_tag="ACHURJ_000174"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFR1279271.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            22958..23329
                     /gene="srlB"
                     /locus_tag="ACHURJ_000175"
                     /gene_synonym="gutB"
     CDS             22958..23329
                     /gene="srlB"
                     /locus_tag="ACHURJ_000175"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFR1279272.1"
                     /translation="MTVIYKTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            23333..24112
                     /gene="srlD"
                     /locus_tag="ACHURJ_000176"
     CDS             23333..24112
                     /gene="srlD"
                     /locus_tag="ACHURJ_000176"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFR1279273.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLVAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGESMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLVEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            24217..24575
                     /gene="gutM"
                     /locus_tag="ACHURJ_000177"
                     /pseudo
     CDS             24217..24575
                     /gene="gutM"
                     /locus_tag="ACHURJ_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
     gene            24642..25415
                     /gene="srlR"
                     /locus_tag="ACHURJ_000178"
                     /gene_synonym="gutR"
     CDS             24642..25415
                     /gene="srlR"
                     /locus_tag="ACHURJ_000178"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFR1279274.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKLASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            25408..26373
                     /gene="gutQ"
                     /locus_tag="ACHURJ_000179"
     CDS             25408..26373
                     /gene="gutQ"
                     /locus_tag="ACHURJ_000179"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFR1279275.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(26370..27884)
                     /gene="norR"
                     /locus_tag="ACHURJ_000180"
     CDS             complement(26370..27884)
                     /gene="norR"
                     /locus_tag="ACHURJ_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFR1279276.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKGVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGMKD"
     gene            28071..29510
                     /gene="norV"
                     /locus_tag="ACHURJ_000181"
     CDS             28071..29510
                     /gene="norV"
                     /locus_tag="ACHURJ_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFR1279277.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNKVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFEELASEAK"
     gene            29507..30640
                     /gene="norW"
                     /locus_tag="ACHURJ_000182"
                     /pseudo
     CDS             29507..30640
                     /gene="norW"
                     /locus_tag="ACHURJ_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
     gene            complement(30768..33020)
                     /gene="hypF"
                     /locus_tag="ACHURJ_000183"
     CDS             complement(30768..33020)
                     /gene="hypF"
                     /locus_tag="ACHURJ_000183"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFR1279278.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLREDPETFLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPKYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLAAFWQQWLNWQAQVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(33173..33700)
                     /gene="hydN"
                     /locus_tag="ACHURJ_000184"
     CDS             complement(33173..33700)
                     /gene="hydN"
                     /locus_tag="ACHURJ_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFR1279279.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(33849..34859)
                     /gene="ascG"
                     /locus_tag="ACHURJ_000185"
     CDS             complement(33849..34859)
                     /gene="ascG"
                     /locus_tag="ACHURJ_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFR1279280.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCNAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIHRDSLIAL
                     SR"
     gene            35119..36576
                     /gene="ascF"
                     /locus_tag="ACHURJ_000186"
     CDS             35119..36576
                     /gene="ascF"
                     /locus_tag="ACHURJ_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HFR1279281.1"
                     /translation="MAKNYAALARSEIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTPRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASADIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYSIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAQARKHQSVQPTVAKEVSLN"
     gene            36585..38009
                     /gene="ascB"
                     /locus_tag="ACHURJ_000187"
     CDS             36585..38009
                     /gene="ascB"
                     /locus_tag="ACHURJ_000187"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFR1279282.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LAAKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKALGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(38168..38638)
                     /gene="hycI"
                     /locus_tag="ACHURJ_000188"
     CDS             complement(38168..38638)
                     /gene="hycI"
                     /locus_tag="ACHURJ_000188"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFR1279283.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(38631..39041)
                     /gene="hycH"
                     /locus_tag="ACHURJ_000189"
     CDS             complement(38631..39041)
                     /gene="hycH"
                     /locus_tag="ACHURJ_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFR1279284.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(39038..39805)
                     /gene="hycG"
                     /locus_tag="ACHURJ_000190"
     CDS             complement(39038..39805)
                     /gene="hycG"
                     /locus_tag="ACHURJ_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFR1279285.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGELDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEVRIR"
     gene            complement(39805..40347)
                     /gene="hycF"
                     /locus_tag="ACHURJ_000191"
     CDS             complement(39805..40347)
                     /gene="hycF"
                     /locus_tag="ACHURJ_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFR1279286.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(40357..42066)
                     /gene="hycE"
                     /locus_tag="ACHURJ_000192"
     CDS             complement(40357..42066)
                     /gene="hycE"
                     /locus_tag="ACHURJ_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFR1279287.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(42084..43007)
                     /gene="hycD"
                     /locus_tag="ACHURJ_000193"
     CDS             complement(42084..43007)
                     /gene="hycD"
                     /locus_tag="ACHURJ_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFR1279288.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(43010..44641)
                     /gene="hycC"
                     /locus_tag="ACHURJ_000194"
     CDS             complement(43010..44641)
                     /gene="hycC"
                     /locus_tag="ACHURJ_000194"
                     /inference="COORDINATES: protein motif:HMM:NF005958.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFR1279289.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKEGKLWFALGRLGTLLLAI
                     ACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWVPQAHANA
                     SAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIVLLVLGMITAFVGGLYALMEHN
                     IQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSLFKSVLFL
                     GAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEWVIYQSFF
                     KLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAENATCAPLL
                     MSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLIACPLLPF
                     IIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKLRKWLNPV
                     SLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            44853..45203
                     /gene="hypA"
                     /locus_tag="ACHURJ_000195"
     CDS             44853..45203
                     /gene="hypA"
                     /locus_tag="ACHURJ_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFR1279290.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEVECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            45207..46079
                     /gene="hypB"
                     /locus_tag="ACHURJ_000196"
     CDS             45207..46079
                     /gene="hypB"
                     /locus_tag="ACHURJ_000196"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFR1279291.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            46070..46342
                     /gene="hypC"
                     /locus_tag="ACHURJ_000197"
     CDS             46070..46342
                     /gene="hypC"
                     /locus_tag="ACHURJ_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFR1279292.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVITEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            46342..47463
                     /gene="hypD"
                     /locus_tag="ACHURJ_000198"
     CDS             46342..47463
                     /gene="hypD"
                     /locus_tag="ACHURJ_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFR1279293.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            47460..48470
                     /gene="hypE"
                     /locus_tag="ACHURJ_000199"
     CDS             47460..48470
                     /gene="hypE"
                     /locus_tag="ACHURJ_000199"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFR1279294.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPCYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            48544..50622
                     /gene="flhA"
                     /locus_tag="ACHURJ_000200"
     CDS             48544..50622
                     /gene="flhA"
                     /locus_tag="ACHURJ_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HFR1279295.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRRILSRPDTLHCSYEEFC
                     ETWPQLVAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(50659..51012)
                     /locus_tag="ACHURJ_000201"
     CDS             complement(50659..51012)
                     /locus_tag="ACHURJ_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFR1279296.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(51089..51223)
                     /locus_tag="ACHURJ_000202"
     CDS             complement(51089..51223)
                     /locus_tag="ACHURJ_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1279297.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            51299..53860
                     /gene="mutS"
                     /locus_tag="ACHURJ_000203"
     CDS             51299..53860
                     /gene="mutS"
                     /locus_tag="ACHURJ_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFR1279298.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAGLQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     DVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            53966..54622
                     /gene="pphB"
                     /locus_tag="ACHURJ_000204"
     CDS             53966..54622
                     /gene="pphB"
                     /locus_tag="ACHURJ_000204"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFR1279299.1"
                     /translation="MPSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFCPETD
                     LLISVGDNIDRGPESLDVLRLLNQPWFISVKGNHEAMELDAFATGDGNMWLASGGDWF
                     FDLNDSEQQEAIDLLLKFHHLPHIIEITNDIIKYVIAHADYPGDEYLFGKEIAESELL
                     WPVYRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIR
                     "
     gene            complement(54673..55440)
                     /gene="ygbI"
                     /locus_tag="ACHURJ_000205"
     CDS             complement(54673..55440)
                     /gene="ygbI"
                     /locus_tag="ACHURJ_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFR1279300.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            55636..56544
                     /gene="ltnD"
                     /locus_tag="ACHURJ_000206"
     CDS             55636..56544
                     /gene="ltnD"
                     /locus_tag="ACHURJ_000206"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HFR1279301.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGDAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            56541..57802
                     /gene="otnK"
                     /locus_tag="ACHURJ_000207"
                     /pseudo
     CDS             56541..57802
                     /gene="otnK"
                     /locus_tag="ACHURJ_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
     gene            57799..58436
                     /locus_tag="ACHURJ_000208"
                     /pseudo
     CDS             57799..58436
                     /locus_tag="ACHURJ_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
     gene            58441..59217
                     /locus_tag="ACHURJ_000209"
     CDS             58441..59217
                     /locus_tag="ACHURJ_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFR1279302.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAMEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFVPHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            59306..60670
                     /locus_tag="ACHURJ_000210"
     CDS             59306..60670
                     /locus_tag="ACHURJ_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HFR1279303.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVSFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVATAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMTGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(60764..61756)
                     /gene="rpoS"
                     /locus_tag="ACHURJ_000211"
     CDS             complement(60764..61756)
                     /gene="rpoS"
                     /locus_tag="ACHURJ_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFR1279304.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGVTQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(61819..62865)
                     /gene="nlpD"
                     /locus_tag="ACHURJ_000212"
     CDS             complement(61819..62865)
                     /gene="nlpD"
                     /locus_tag="ACHURJ_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFR1279305.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGSYSGSTYTVKKGDTLFY
                     IAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITGGNAITQADAAEQGVVI
                     KTAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPAATTVTAPVTVPTASTTEPTVS
                     STSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQAIIATADGRVVYAGNAL
                     RGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATMGSTGTSSTRLHFEIRY
                     KGKSVNPLRYLPQR"
     gene            complement(63050..63676)
                     /gene="pcm"
                     /locus_tag="ACHURJ_000213"
     CDS             complement(63050..63676)
                     /gene="pcm"
                     /locus_tag="ACHURJ_000213"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFR1279306.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(63670..64431)
                     /gene="surE"
                     /locus_tag="ACHURJ_000214"
     CDS             complement(63670..64431)
                     /gene="surE"
                     /locus_tag="ACHURJ_000214"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFR1279307.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(64412..65461)
                     /gene="truD"
                     /locus_tag="ACHURJ_000215"
     CDS             complement(64412..65461)
                     /gene="truD"
                     /locus_tag="ACHURJ_000215"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFR1279308.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQHRVNDKELMITAALPGSGEWGTQRKALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(65458..65937)
                     /gene="ispF"
                     /locus_tag="ACHURJ_000216"
     CDS             complement(65458..65937)
                     /gene="ispF"
                     /locus_tag="ACHURJ_000216"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFR1279309.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(65937..66647)
                     /gene="ispD"
                     /locus_tag="ACHURJ_000217"
     CDS             complement(65937..66647)
                     /gene="ispD"
                     /locus_tag="ACHURJ_000217"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFR1279310.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(66666..66977)
                     /gene="ftsB"
                     /locus_tag="ACHURJ_000218"
     CDS             complement(66666..66977)
                     /gene="ftsB"
                     /locus_tag="ACHURJ_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFR1279311.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(67171..67494)
                     /locus_tag="ACHURJ_000219"
     CDS             complement(67171..67494)
                     /locus_tag="ACHURJ_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFR1279312.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(67544..68149)
                     /gene="cysC"
                     /locus_tag="ACHURJ_000220"
     CDS             complement(67544..68149)
                     /gene="cysC"
                     /locus_tag="ACHURJ_000220"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFR1279313.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(68149..69576)
                     /gene="cysN"
                     /locus_tag="ACHURJ_000221"
     CDS             complement(68149..69576)
                     /gene="cysN"
                     /locus_tag="ACHURJ_000221"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFR1279314.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTHIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMSWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKALPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     PSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYPVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPKNEAAVASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(69578..70486)
                     /gene="cysD"
                     /locus_tag="ACHURJ_000222"
     CDS             complement(69578..70486)
                     /gene="cysD"
                     /locus_tag="ACHURJ_000222"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFR1279315.1"
                     /translation="MAQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            70738..71775
                     /gene="iap"
                     /locus_tag="ACHURJ_000223"
     CDS             70738..71775
                     /gene="iap"
                     /locus_tag="ACHURJ_000223"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFR1279316.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAKRLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            complement(71982..72275)
                     /gene="cas2e"
                     /locus_tag="ACHURJ_000224"
     CDS             complement(71982..72275)
                     /gene="cas2e"
                     /locus_tag="ACHURJ_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HFR1279317.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(72275..73195)
                     /gene="cas1e"
                     /locus_tag="ACHURJ_000225"
     CDS             complement(72275..73195)
                     /gene="cas1e"
                     /locus_tag="ACHURJ_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HFR1279318.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLQVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPPPARRSVEQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRG"
     gene            complement(73192..73842)
                     /gene="cas6e"
                     /locus_tag="ACHURJ_000226"
     CDS             complement(73192..73842)
                     /gene="cas6e"
                     /locus_tag="ACHURJ_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFR1279319.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPSGKERQ
                     FLYRREELQGAFRFFVLSQERPAESDTFTIECRSFAPELCTGQQLCFNLRANPTICKA
                     GKRHDLLMEAKRQVRGQAEGSDVWLHQQQAALDWLAAQGERSGFTLLDISVDAYRQQQ
                     LRRENSQQLIQFSSVDYTGMLTVTDPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA"
     gene            complement(73824..74570)
                     /gene="cas5e"
                     /locus_tag="ACHURJ_000227"
     CDS             complement(73824..74570)
                     /gene="cas5e"
                     /locus_tag="ACHURJ_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HFR1279320.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSLSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            complement(74581..75659)
                     /gene="cas7e"
                     /locus_tag="ACHURJ_000228"
                     /pseudo
     CDS             complement(74581..75659)
                     /gene="cas7e"
                     /locus_tag="ACHURJ_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
     gene            complement(75674..76242)
                     /gene="casB"
                     /locus_tag="ACHURJ_000229"
                     /gene_synonym="cse2"
                     /pseudo
     CDS             complement(75674..76242)
                     /gene="casB"
                     /locus_tag="ACHURJ_000229"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029315.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
     gene            complement(76239..>76634)
                     /gene="casA"
                     /locus_tag="ACHURJ_000230"
                     /gene_synonym="cse1"
     CDS             complement(76239..>76634)
                     /gene="casA"
                     /locus_tag="ACHURJ_000230"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HFR1279321.1"
                     /translation="LIPKLRLAAQAASHILSLLHRALKEAWFSEKKTTKLDFGFVDID
                     FWNKTQHRFLRLVRKIEEGQDPDELLSKWQKEMWLFARQDFDDRVFTNPYEPVDLERV
                     MTARKKYFTTSAEKQNANAAREKKQEAAE"
BASE COUNT        18032 a        20779 c        19666 g        18157 t
ORIGIN      
        1 gctcctctta tgtgatgggg aaagcggttg aaacagaaga tgaagactgg aatttctgag
       61 ggtgttattt tcaaaaatat cactacccgc agcagggaaa taattcccgc caaatagctt
      121 tttatcacgc aaatattctg tagtgatcta cactgagact ctgttgcatt attcgcctga
      181 aactacaata ttcaggcgtt ttttcgctat ctatgacaaa aaatatcaac ttttctcgat
      241 ttgctctcag cccttatatc acgggaaatt ccggcgattt gctcgcatca atattcatgc
      301 cacatttgcc ctcaggggtt gcctcagatt ctcagtatgt tagggtagaa aaaagtgact
      361 atttccattg ggtaatatat cgacataaac aaataaagga atctttctat tgcatggcaa
      421 ttaaattaga aattaaaaat ctttataaaa tatttggcga gcatccacag cgagcgttca
      481 aatatatcga acaaggactt tcgaaagaac aaattctgga aaaaactggg ctatcgcttg
      541 gcgtaaaaga cgccagtctg gccattgaag aaggcgagat atttgtcatc atgggattat
      601 ccggctcggg taaatccaca atggtacgcc ttctcaatcg cctgattgaa cccacccgcg
      661 ggcaagtgct gattgatggt gtggatattg ccaaaatatc cgatgccgaa ctccgtgagg
      721 tgcgcagaaa aaagattgcg atggtcttcc agtcctttgc cttaatgccg catatgaccg
      781 tgctggacaa tactgcgttc ggtatggaat tggccggaat taatgccgaa gaacgccggg
      841 aaaaagccct tgatgcactg cgtcaggtcg ggctggaaaa ttatgcccac agctacccgg
      901 atgaactctc tggcgggatg cgtcaacgtg tgggattagc ccgcgcgtta gcgattaatc
      961 cggatatatt attaatggac gaagccttct cggcgctcga tccattaatt cgcaccgaga
     1021 tgcaggatga gctggtaaaa ttacaggcga aacatcagcg caccattgtc tttatttccc
     1081 acgatctcga tgaagctatg cgtattggcg accgaattgc cattatgcaa aatggtgaag
     1141 tggtacaggt cggcacaccg gatgaaattc tcaataatcc ggcgaatgat tatgtccgta
     1201 ccttcttccg tggcgttgat attagtcagg tattcagtgc gaaagatatt gcccgccgga
     1261 caccgaatgg cttaattcgt aaaacccctg gcttcggccc acgttcggca ctgaaattat
     1321 tgcaggatga agatcgcgaa tatggctacg ttatcgaacg cggtaataag tttgtcggcg
     1381 cagtttccat cgattcgctt aaaaccgcgt taacgcagca gcaaggtctt gatgcggcgc
     1441 tgattgatgc gccgttagca gtcgatgcac aaacgcctct tagcgagttg ctctctcatg
     1501 tcggacaggc accctgtgcg gtacccgtgg tcgacgagga ccaacagtat gtcggcatca
     1561 tttcgaaagg aatgctgctg cgcgctttag atcgtgaggg ggtaaataat ggctgatcaa
     1621 aataatccgt gggataccac gccagcggcg gacagtgccg tgcaatccgc agacgcctgg
     1681 ggtacaccgg cgactgcacc gactgacggc ggtggtgctg actggctgac cagtacgcct
     1741 gcgccaaacg tcgagcattt taatattctc gatccgttcc ataaaacgct gatcccgctc
     1801 gacagttggg tcactgaagg gatcgactgg gttgttaccc atttccgtcc cgtcttccag
     1861 ggcgtgcgcg ttccggttga ttatatcctc aacggtttcc agcaattgct gctgggtatg
     1921 cccgcaccgg tggcgattat cgttttcgct ctcatcgcct ggcagatttc cggggtcgga
     1981 atgggcgtgg cgacgctggt ttcgctgatt gccatcggcg caatcggtgc ctggtcgcag
     2041 gccatggtta ccctggcgct ggtgttaacc gccctgctgt tctgtatcgt cattggtttg
     2101 ccgctgggga tctggctggc gagaagtccg cgagcggcga aaattattcg tccactgctt
     2161 gatgccatgc agaccacgcc agcgtttgtt tatctggtgc caatcgtcat gctatttggt
     2221 atcggtaacg tgccgggcgt ggtggtgacg atcatctttg ctctgccgcc gattatccgt
     2281 ctgaccattc tggggattaa ccaggttccg gcggatctga ttgaagcctc gcgctcattc
     2341 ggtgccagcc cgcgccagat gctgttcaaa gttcagttac cgctggcgat gccgagcatt
     2401 atggcgggcg ttaaccagac gctgatgctg gccctttcta tggtggtcat tgcctcgatg
     2461 attgccgtcg gcgggctggg tcagatggta cttcgcggta tcggtcgtct ggatatgggg
     2521 cttgccaccg ttggcggcgt cgggattgtg atcctcgcca ttatcctcga tcgcctgacg
     2581 caggccgttg ggcgcgactc acgcagtcgc ggcaaccgtc gctggtacac cactggccct
     2641 gttggtctgc tgacccgccc attcattaag taactctgca cttgcccggt gacgccgggc
     2701 attatcaccc tgccaaaaaa aggaataaca atgcgacata gcgtactttt tgcgacagcg
     2761 tttgccacgc ttatctctac acaaactttt gctgccgatc tgccgggcaa aggtattact
     2821 gttaatccag ttcaaagcac catcaccgaa gaaaccttcc agacgctgct ggtcagccgt
     2881 gcgctggaga aattaggcta taccgtcaac aaacccagcg aagtagatta caacgttggc
     2941 tacaccttgc ttgcttccgg cgatgcaacc ttcaccgccg tgaactggac gccactgcat
     3001 gacaacatgt acgaagctgc cggtggcgat aagaaatttt atcgtgaagg ggtatttgtt
     3061 aacggcgcgg cacagggtta cctgatcgat aagaaaaccg ccgaccagta caaaatcacc
     3121 aacatcgcac aactgaaaga gccgaagatc gccaaactgt tcgataccaa cggcgacggt
     3181 aaagcggatt taaccggttg taatcctggc tggggctgcg aaggtgcgat caaccaccag
     3241 cttgccgcgt atgaactgac caacaccgtg acgcataatc aggggaacta cgcagcgatg
     3301 atggccgaca ccatcagtcg ctacaaagag ggcaaaccgg tgttttatta cacctggacg
     3361 ccgtactggg tgagtaatga gctgaagcca gggaaagatg tggtctggtt gcaggtgccg
     3421 ttctccgcac tgccgggcga taaaaatgcc gataccaaac tgccgaatgg cgcgaattac
     3481 ggcttcccgg tcagcaccat gcatatcgtt gccaacaaag cctggaccga gaaaaatccg
     3541 gcggcagcca aactgtttgc cattatgcag ttgccagtgg ccgatattaa cgcccagaac
     3601 gccattatgc atgacggcaa agcctcagaa ggcgatattc agggccacgt tgatggctgg
     3661 atcaaagccc accagcagca gttcgatggc tgggtgaatg aggcgctggc agcgcagaag
     3721 taatttttat tcaggcggat aaggcgtttt tgccttatcc gccgctctgt acacaatgcc
     3781 tgatgcgacg ctgacgcgtc ttatcatgcc tacaaaatac actcattccg tatggcggat
     3841 aaggcgtttt cgccgcatcc gccgttctgt gcacaatgcc tgatgcgacg ctggcgcgtc
     3901 ttatcatgcc tacaaaccac atcgcacatt tcaacaagct atctttcatc ccatattcat
     3961 caacatccgc tattattgat ttccagctta atcatcacct gatgaacaaa aataatgact
     4021 aaacctaatc atgagcttag cccggcgctg atcgtgctga tgtctatcgc caccggtctg
     4081 gcggtagcca gtaactatta cgcccagcca ttgctcgaca ccatcgcgcg taacttttcc
     4141 ctttccgcca gttcggcagg ctttattgtt accgccgcgc aattgggcta tgcagcaggt
     4201 ctgctgtttc ttgttcccct cggtgatatg tttgaacgcc gccgcctgat tgtctcgatg
     4261 accttactgg cggcaggcgg catgttaatc actgccagca gtcagtcgct ggcgatgatg
     4321 atcctcggta cggcattaac cggtttattc tctgtcgtgg cacaaattct ggttccgctg
     4381 gcagcgacgc tggcttcacc ggacaaacgc ggcaaagtgg ttggcactat tatgagcggt
     4441 ctgctgttgg ggatcttact cgcacggacc gttgccggat tgctggcgaa tctcggcggc
     4501 tggcgcaccg tcttttgggt tgcctcggtg ttaatggcac tgatggcgct ggcattatgg
     4561 cgtggtctgc cacaaatgaa atcagaaacc cacctcaact acccacagtt gttaggttcc
     4621 gttttcagca tgtttatcag cgataagatc ctgcgcaccc gcgcattgct gggctgcctg
     4681 acctttgcca acttcagcat tctctggacc tcaatggcct ttttgcttgc cgctccacct
     4741 tttaactaca gcgatggtgt cattggtctg tttggacttg cgggagctgc cggagcgttg
     4801 ggcgctcgtc cggcgggcgg ttttgccgat aagggtaaat cgcaccacac cacaactttc
     4861 ggtctgctgc tgctattact ttcatggctg gcgatctggt ttggtcacac ttccgtactg
     4921 gcgctgatta tcggcatcct ggtgctggat ctcaccgtac agggcgtgca tatcactaac
     4981 cagacggtaa tttatcgaat acaccctgat gcgcgtaatc gcctgaccgc aggttacatg
     5041 accagctact ttattggcgg tgccgccggt tcgctaattt cagcctcagc ctggcaacat
     5101 ggcggttggg ctggcgtttg tctggctggc gcgacgattg ccctggttaa cttactggtc
     5161 tggtggcgag gttttcatcg tcaggaagcc gcaaattaag caatcagcaa ccgttttcgt
     5221 ggctttacac ttataagggt gttaagaagc ccatcagtct gataaggtta agatattcat
     5281 tcagtctatt tataatatta acaatcgtta agcgtacact ctatggaaag ccctactcca
     5341 cagcctgctc ttggttcggc gaccttcatg gaaggatgca aagacagttt accgattgtt
     5401 attagttata ttccggtggc ctttgcgttc ggtctgaatg cgacccgtct gggattctct
     5461 cctctcgaaa gcgttttttt ctcctgcatc atttatgcag gcgcgagcca gttcgtcatt
     5521 accgcgatgc tggcagccgg gagtagtttg tgggttgctg cactgaccgt catggcaatg
     5581 gatgttcgcc atgtgttgta tggcccgtca ctgcgtagcc gtattattca gcgtctgcaa
     5641 aaatcgaaaa ccgccctgtg ggcatttggc ctgacggatg aggtttttgc cgccgcaacc
     5701 gcaaaactgg tacgcaataa tcgccgctgg agcgagaact ggatgatcgg cattgccttc
     5761 agttcatggt catcgtgggt atttggtacg gtaatagggg cattctccgg cagcggcttg
     5821 ctgcaaggtt atcccgccgt tgaagctgca ttaggtttta tgcttccggc actctttatg
     5881 agtttcctgc tcgcctcttt ccagcgcaaa caatctcttt gcgttaccgc agcgttagtt
     5941 ggtgcccttg caggcgtaac gctattttct attcccgtcg ccattctggc aggcattgtc
     6001 tgtggctgcc tcactgcgtt aatccaggca ttctggcaag gagcgcccga tgagctatga
     6061 ggttctgctg cttgggttac tggttggcgt ggcgaattat tgcttccgct atttgccgct
     6121 gcgcctgcgt gtgggtaatg cccgcccaac caaacgtggc gcggtaggta ttttgctcga
     6181 caccattggc atcgcctcga tatgcgctct gctggttgtc tctaccgcac cagaagtgat
     6241 gcacgataca cgccgtttcg tgcccacgct ggtcggcttc gcggtactgg gtgccagttt
     6301 ctataaaaca cgcagcatta tcatcccaac actgcttagt gcgctggcct atgggctcgc
     6361 ctggaaagtg atggcgatta tataacccac aagaatcatt tttctaaaac aatacattta
     6421 ctttatttgt tactgtcgtt actatatcgg ctgaaattaa tgaggtcata cccaaatgga
     6481 tagttcgttt acgcccattg aacaaatgct aaaatttcgc gccagccgcc acgaagattt
     6541 tccttatcag gagatccttc tgactcgtct ttgcatgcac atgcaaagca agctgctgga
     6601 gaaccgcaat aaaatgctga aggctcaggg gattaacgag acgttgttta tggcgttgat
     6661 tacgctggag tctcaggaaa accacagtat tcagccttct gaattaagtt gtgctcttgg
     6721 atcatcccgt accaacgcga cgcgtattgc cgatgaactg gaaaaacgcg gttggatcga
     6781 acgtcgtgaa agcgataacg atcgccgctg cctgcatctg caattaacgg aaaaaggtca
     6841 cgagtttttg cgcgaggttt taccaccgca gcataactgc ctgcatcaac tctggtccgc
     6901 gctcagcaca acagaaaaag atcagctcga gcaaatcacc cgcaaattgc tctcccgtct
     6961 cgaccagatg gaacaagacg gtgtggttct cgaagcgatg agctaacgcg tcatctcgct
     7021 caaaaatcca gatttataaa agaaaaaatg accggccagc atcgcaacat gctggccttt
     7081 ttggcaagca ggtcggctca gccgatgagt taagaagatc gtggagaaca atatgagcgc
     7141 aaatgcggag actcaaaccc cgcagcaacc ggtaaagaag agcggcaaac gtaagcgtct
     7201 gctcctcctt ctcaccttgc tctttataat tattgccgta gcgataggga tttattggtt
     7261 tttggtactg cgtcatttcg aagaaaccga tgacgcatac gtggcaggga atcagattca
     7321 aattatgtct caggtgtctg gcagcgtgac gaaagtctgg gccgataaca ccgattttgt
     7381 aaaagaaggc gatgttctgg tcactctcga cccgacagat gctcgccagg cgtttgaaaa
     7441 agccaaaact gcactggctt ccagcgttcg ccaaacccac cagctgatga ttaacagcaa
     7501 acagttacag gcgaatattg aggtgcaaaa aatcgccctc gcgaaagcac aaagcgacta
     7561 caaccgccgt gtgccgctgg gcaatgccaa cctgattggt cgcgaagagc tgcaacacgc
     7621 ccgcgacgcc gtcaccagtg cccaggcgca actggacgtc gctattcaac aatacaatgc
     7681 caatcaggcg atgattctgg ggactaaact ggaagatcag cctgccgtgc aacaggctgc
     7741 caccgaagta cgtaacgcct ggctggcgct ggagcgtacc cgtattgtca gtccgatgac
     7801 cggttatgtc tcccgccgcg cggtacagcc tggggcgcaa attagcccaa cgacgccgct
     7861 gatggcggtc gttccagcca ccaatatgtg ggtggatgcc aactttaaag agacgcagat
     7921 tgccaatatg cgtatcggtc agccggtcac tatcaccacg gatatttacg gcgatgatgt
     7981 gaaatacacc ggtaaagtgg ttggtctgga tatgggcaca ggtagcgcat tctcactgct
     8041 tccggcgcaa aatgcgaccg gtaactggat caaagtcgtt cagcgtctac ctgtgcgtat
     8101 cgaactggac cagaaacagc tggagcaata cccgctgcgc attggtttgt ccacgctggt
     8161 gagcgtcaat accactaacc gtgacggtca ggtactggca aataaagtac gttcaacacc
     8221 ggtcgcggta agtaccgcgc gtgaaatcag cctggcacct gtcaataaac tgatcgacga
     8281 tatcgtaaaa gctaacgctg gctaatccag aggtgcgtgt gatgcaacag caaaaaccgc
     8341 tggaaggcgc gcaactggtc attatgacga ttgcgctgtc actggcgaca ttcatgcagg
     8401 tgctggactc caccattgct aacgtggcga tccccactat cgccgggaat ctgggctcat
     8461 cgctcagcca gggaacgtgg gtaatcactt ctttcggggt ggcgaatgcc atctcgatcc
     8521 cgcttaccgg ctggctggca aagcgcgtcg gggaagtgaa actgttcctt tggtccacca
     8581 tcgcctttgc tattgcgtcg tgggagtgtg gtgtctccag cagcctgaat atgctgatct
     8641 tcttccgtgt gattcagggg attgtcgctg ggccgttgat cccactttcg caaagtcttt
     8701 tgctgaataa ttacccgcca gccaaacgct caatcgcgct ggcgttgtgg tcgatgacgg
     8761 tgattgtcgc gccaatttgc ggcccaatcc tcggcggtta tatcagcgat aattaccact
     8821 ggggctggat attcttcatc aacgtgccaa ttggcgtggc ggtggtgttg atgacactgc
     8881 aaaccctgcg cggacgcgaa acccgcaccg aacggcggcg gattgatgcc gtggggctgg
     8941 cactgctggt tattggtatc ggtagcctgc agattatgct cgaccgcggt aaagagctgg
     9001 actggttttc atcacaggaa attatcattc ttaccgtggt ggcggtggtg gctatctgct
     9061 tcctgattgt ctgggagctg accgacgata acccgatagt cgatctgtcg ttgtttaagt
     9121 cgcgcaactt caccatcggc tgcttgtgta tcagcctcgc gtatatgctc tacttcggcg
     9181 cgattgttct gctgccgcag ttgttgcagg aggtctacgg ttacacggcg acctgggcag
     9241 gtttggcctc tgcgccggta gggattattc cggtgatcct gtcgccgatt atcggccgct
     9301 tcgcgcataa actggatatg cggcggctgg taaccttcag ctttattatg tatgccgtct
     9361 gcttctactg gcgtgcctat acctttgaac caggtatgga ttttggcgcg tcggcctggc
     9421 cgcagtttat ccaggggttt gcggtggcct gcttctttat gccgctgacc accattacgc
     9481 tgtctggttt gccaccggaa cgactggcgg cggcatcgag cctctctaac tttacgcgaa
     9541 cgctggcggg gtctatcggc acgtcgataa ccacgaccat gtggaccaac cgcgagtcga
     9601 tgcaccatgc gcagttgact gagtcggtaa acccgttcaa cccgaatgcc caggcgatgt
     9661 acagtcaact ggaagggctt gggatgacgc aacagcaggc atcaggctgg attgcccagc
     9721 agatcactaa tcaggggctg attatttccg ccaatgagat cttctggatg tcagccggga
     9781 tattcctcgt cctgctgggg ctggtgtggt ttgctaaacc gccctttggc gcaggtggcg
     9841 gcggaggtgg tgcgcactaa gtacaactaa gccagttcat ttgaactggc ttttttcaat
     9901 taattgtgaa gatagtttac tgactaaatg tgcagttcct gcaacttctc tttcggcagt
     9961 gccagttctt cgttgctgtt gatgcgtacg tcacgttcca gaatgctacg cgcaatatcc
    10021 tgcgcttcct gcaacgagtg catctggtaa gtgccacact ggtagacgtt cagctccggg
    10081 atctgattct gatcctgcac tttcagcacg tcttccattg ccgctttcca ggcatcagca
    10141 acacgctgct catctggcgt accaatcaga ctcatataaa aaccggtgcg gcagcccatt
    10201 ggcgagatat cgataatctc tacaccatta ccgttaagat ggttacgcat aaaaccagca
    10261 aacaggtgct ccagagtatg gctccctctt tctggcatca cttctttgtt cggcacgcag
    10321 aagcgcagat cgaacacggt gattgcgtcg ccatgcgggg tgttcattgt tttcgccacc
    10381 cgaactgcag gcgcttccat ccgggtatga tcgactgtga agctatctaa caacggcatt
    10441 tagccacctc cggtaatttt tttaaaaatt ttctgaactc tttcttccca ggcgagtctg
    10501 agtatatgaa agacgcgcat ttgttatcat catccctgaa ttcagagatg aaattttggc
    10561 cactcacgag tggccttttt cttttctgtc aggcgtgttt ttccagccac accgcaaacg
    10621 gttcggtatc agcggtttcc atttcctgct gacggcgttc agatgcctcg cgctcggcta
    10681 caaaatcctc ttcgcgcaga atttccaacg gctcttcacg cagcagatta cggtaggctt
    10741 ctgcaaacgc tttgcctgtt ccgccaatac cagtatcaat catagacctt aagatacggg
    10801 cagagaaagt cagatcggga ttatcgaagc aggcgaccag ttcatcacac actttctgat
    10861 acgctttgcc gccgttaatg ctatccaacg tttgcgcgac gcgtttcaga tcgcggaaca
    10921 gatctttacc cacctgcggt aacgggaact gcgcggtttc gcagccgata cccagcgtca
    10981 ggcccggttt gcgaccttcg aggatcaccc ggttccagtt aacgcgtgta caggcaagtt
    11041 cgctactgct catttccggt gcatcagcca gcgcacacca gaccataaac aggtcgagga
    11101 atcgcacctg ctgttcatct acaccaatcg gcgagaacgg gttgatgtcc agcgaacgca
    11161 cttcaatata ttcaatgccg ccacgtaaca gcgcatcaga aggtgactcg ccgctgcggg
    11221 taacgcgttt tggacgaatc ggcgcataca gttcgttttc aatctgcagc acgttgctat
    11281 tgatttgcag cctcttaccg tctttctcaa taccaatctt cgcgtactct tccgatggcg
    11341 ttttgattgc ctgtttaagg cccgctacgt actcgtaaag atcgttgaag gtaataccaa
    11401 gattgctttg cgatttattg gtatagccga gatcgctcaa acgaagagag gtcgcatacg
    11461 gcaggtaata cataccgcac tcggttttct caaacggcag cgacgttggt tttccttgca
    11521 ggaaagaaga acaaatcgcc ggagatgcac caaacagata aggaatgacc caaccgaaac
    11581 gatagtaatt gcggataacg cggaaatagc ccgcagaaat tttctctttg gcatcagtgc
    11641 ccgagatatc acagcacttc gcttgccaga atgccattgg caaagagaaa ttgtagtgca
    11701 cgccggaaat ggtttgcatc agcgcgccgt agcgattttt cagcccttca cgatacagcg
    11761 ttttaaagcg tccggtgtta gaagtgccgt actgtgccag ttcgatgtcc tgaccttctg
    11821 cgatgtagca tggcatactt aacggccaca tccgctcatc gcccatattg cgcgccgtat
    11881 aacgatgcag atcgcgcata aaggtcaaca tatgttcaat atcaccatcc actggtgtaa
    11941 tgaattccag caatgcttcc gcaaaatcgg tagtaatcca tttgtgcgtc agtgcggaac
    12001 ctaatgcttc aggatgacct gttgttgcct gtgtgccatc agcattaaca cgcaaagttt
    12061 cgcgctccag cccacgctgt atccccttta acgcctgagg atgtttttcc agccaggcca
    12121 gcgcctgtga tacgtccggg atcaaattga cctcccgcct gtcaaaatcg ttttaattag
    12181 cataactgta atggtgacca tatgtgcagg cctacaatta gtgccaccac atcatgccct
    12241 gaacggtagc tgctgcaact gcaacatagc gtaaagcttt accaaggcac aaaaaaaaga
    12301 ttaccggtcc ccacgagatg cgcatccatc ccgctaacag acacagtaaa tcgccaacca
    12361 cgggcatcca gcttaataat agcgtgactg caccatagcg tttcagccag ccagtagctt
    12421 tctcttgcca gcgcgatgtt ttacgcaatg gaaagaaacg cccaaggata acgttagtta
    12481 accctccaag gctattaccc attgttgctg ttaaaactaa aacccaggga taactgatcc
    12541 cggaaagcaa cattgccacc agcacgactt cggagttgcc gggtaatagt gtagcgctga
    12601 gaaaactact ggcaaacaac gagaaaagcg ataacgcttc actcacagca agcgaacatc
    12661 cacggcgtcc atgcctgctg cacgggccgc ctgaataccg aaatcggcat cttcaaagac
    12721 cacacactgc gtcggttgca cgcccatacg ctgcgcgcac aacaaaaatg tgtctggcgc
    12781 gggtttatgg tgtttgacgt gatcagcagc gacgacggcg tcaaaataac ggcgtaatcc
    12841 caggtgcgcc agcaatgcct cagcgatggc gctttcactc cccgttccta cagccattgg
    12901 gcgacgacca tgccaacttt tcaccacttc aacaagagga agcggctcga cactatccag
    12961 cagcatactt cttactgctt ctgttttttc acgcgctaac gcatgcgggg cgagatcggc
    13021 ctgattcagc tcaataattg cctgagcaat acgccaggtg ggcgatccat taagcgcaat
    13081 cattgcctga acatcgtact gaagaccgta gtgccctaat acttcgcgcc acgctttacg
    13141 gtgcgtaggc tccgtatcca ggaatatcaa aaattaaacc tgcataacgc tcgtacatgg
    13201 tcttctcgca agtcgaaaaa tatgacgcta ctttagcgta attgcttgat tttgtcgctg
    13261 atggcgaagg agaagaaaat tcaggaaatg gataaagtag ttatttagaa atgagatatt
    13321 tttgaaggaa atttttggga gaagatggtg catccgggag gattcgaacc tccgaccgct
    13381 cggttcgtag ccgagtactc tatccagctg agctacggat gcatcgggaa aatttatttt
    13441 actgctgata ttgatatcgc tactaaagcc atatcaagta agagatggtg catccgggag
    13501 gattactcgc cttgcggctc gcccttcggg ccgttgctaa agcaacgtta tcatccctgg
    13561 tgcctgcggt taattccgct acctcttcga taccttcatt gctgaagatt tcggagaata
    13621 tggtgcatcc gggaggattc gaacctccga ccgctcggtt cgtagccgag tactctatcc
    13681 agctgagcta cggatgcatc gggaaactta ctttactgca gattttttga taccgctact
    13741 aaagccgtat caagtaagag atggtgcatt cgggaggatt actcgccttg cggctcgccc
    13801 ttctggccgt tgctaaagca acgttatcct ccctggtgcc tgcggttata ccgccacctc
    13861 ttccatacct tcatcgctga aagtacgtag aatatggtgc atccgggagg attcgaacct
    13921 ccgaccgctc ggttcgtagc cgagtactct atccagctga gctacggatg caaatggcgg
    13981 tgaggcgggg attcgaaccc cggatgcagc ttttgaccgc atactccctt agcaggggag
    14041 cgccttcagc ctctcggcca cctcaccaca cgcctcttac gagtgcttcg aagaacttgt
    14101 ttatcgctca tcgtcgctgc gtggcgcaca tattactttc tgagacttat aagtcaaaca
    14161 atttttccca cacttttatc gtttgcacac ttcacgctca attagtctgt aaaaacgtca
    14221 aaaagagtgt tttatctaca gaaaaacgga ggtctggcag atagcagtaa tgcaaaaaaa
    14281 tggagactta agttgaatga acgggagtaa agcgaaaaga ctatagagtg aaggagaaat
    14341 tttgagggtg cgtctcaccg ataaagatga gacgcggaaa gattagtaac tggactgctg
    14401 ggatttttca gcctggatac gctggtagat ctcttcacgg tgaacagaaa cttccttcgg
    14461 ggcatttacg ccaatacgta cctggttgcc cttcacccct aaaactgtca cggtgacctc
    14521 atccccaatc atgagggtct caccaactcg acgagtcaga atcagcattc tttgctcctt
    14581 gaaagattaa aagagtcggg tctctctgta tcccggcatt atccatcata taacgccaaa
    14641 aagtaagcaa tgacaaacac attacatgta agcagtcatg gcattacatt ctgttaaacc
    14701 taagtttagc cgatatacac aacttcaacc tgactttatc gttgtcgata gcgttaatgc
    14761 gaatgccgtg aagcgagtcc acggcattgc ctgacgctta tattattgca atttcgcgct
    14821 gacccagcct ttcacactgg ctaacgctgc aggtaaggcc gcagcatccg taccaccggc
    14881 ttgcgccatg tcaggacgtc caccaccctt gccgcccacc tgctgagcga ccataccaat
    14941 cagttcccct gctttcacac gatctgtgac gtccttagat acgcctgcaa tcagagaaac
    15001 cttaccttcg gctaccgttg ccagcacgat aattgtcgac cccagctgat tttttaaatc
    15061 gtcaaccatg gtacgcaaca ttttcggctc aacaccgcta agctcgctaa ccaacagctt
    15121 aacaccatta acatcaattg ccttactgga aagatttgcg ctctcctgtg cggcagcttg
    15181 ttctttaagc tgttgtagtt ctttttctaa ctgacgcgta cgttccagta ctgagcgcac
    15241 tttatccgcc agattattgc tatcgccttt cagcagatgc gcgacttcgc ttaagcgatc
    15301 gctgtctgca tgaacggtgg tgatagcgcc ttctccggtt accgcttcga tacgacgaac
    15361 gcctgcagca gtacccgatt cagagatgat gcggaacaga ccaatatcac cagtgcggct
    15421 ggcgtgagta ccgccacaca gctcggtgga gaaatcgccc atgctcagca cgcgtacacg
    15481 ctcatcatac ttctcgccga acagcgccat cgcacctttc gctttcgccg cttcgagatc
    15541 catgatgttg gtttcgatcg gcaaattgcg acgaatctgt gcgttcacca ggtcttcgac
    15601 cgcacgaatc tcttccggtt tcatcgcttc gttgtgtgag aagtcgaagc gcagcacctt
    15661 gtcgttaacc agtgaacctt tctgcgatac atgagtcccc agaacctggc gcagcgcagc
    15721 gtgcatcagg tgcgttgcgg agtgattcag acgaatacgg gcgcgacgag cctcatcaac
    15781 atcagcctgc acagcgtcgc ccactttcag agaacccgca gcaagtttac cgatgtgacc
    15841 aatcgcctgg ccgtatttct gcgtatcttc caccgcgaag gagaagttag cgtctttcag
    15901 ttcgccttta tcaccaacct gaccgccgga ttccgcatag aatggtgttt gatctagcac
    15961 gaccacagct tcctggcctg cattgatggc atcaaccgct ttaccatcaa caaacagcgc
    16021 ggtcactttg ccgttcagtt ccagatggtc atagccttta aattcagatg cactatcaac
    16081 acggatcatt gcgttgtaat cggcaccaaa gccgctggct tcgcgcgcgc gacgacgctg
    16141 ctcttccatt gctgcttcaa aaccagcttc gtcaactttg atgttgcgct cacgacaaac
    16201 atcagccgtc aggtcaaccg ggaagccata ggtgtcgtac agacggaaag ccgtttcacc
    16261 atccagcgta tcaccagaaa gttttgccag ctcttcatcc agcaacgcca gaccgcgctc
    16321 cagagtacgg gcaaactgct cttcttcagt cttcagcacc tgctcaacct gcgcctgctg
    16381 gcgtttcagg tcttcacccg cagagcccat aacgtcgatc agcggaccaa ccagtttgta
    16441 gaagaaggtt tctttcgcgc cgagcatatt gccgtgacgc actgcgcgac gaatgatacg
    16501 acgcagtaca taaccacggt tttcattgga cggcattacg ccatccgcga tcaggaacgc
    16561 acaagaacga atgtggtcag cgattacgcg cagcgattta ttgctcagat cggtcgcgcc
    16621 agtgactttc gctaccgcct ggatcaacgt gcggaacagg tcgatgtcat agttagagtt
    16681 aacgtgttgc agcaccgcag caatacgctc cagacccata ccggtatcta cagacggctt
    16741 cggcagcggt tccatcgtgc catctgcctg gcggttgaac tgcatgaaga cgatgttcca
    16801 gatctcaatg tagcggtcgc cgtcttcttc cggacttccc ggagggccac cccaaatgtg
    16861 gtcgccgtga tcgtagaaga tttcggtgca cgggccgcac ggaccagtgt cacccatctg
    16921 ccagaagttg tcagatgcgt atggcgcacc tttgttatcg ccgatgcgaa taatacgttc
    16981 gcgcgggatc ccgacttctt tttcccagat ttcgtaggct tcgtcgtcgc tttcatagac
    17041 ggtaacccac agacgctctt tcggcagggc aaaccatttt tcgctggtca gcagttccca
    17101 tgcaaactga atggcatcgt gtttgaaata gtcgccgaag ctgaagttgc ccagcatttc
    17161 gaagaaggta tggtgacgcg cggtgtaacc gacgttttcc aggtcgttgt gtttaccacc
    17221 cgcacgcacg cagcgttggg aagtggtagc gcgggaataa ttacgcttgt cgagcccaag
    17281 gaacacatcc ttgaactgat tcatcccggc gttggtaaac aacaaagttg ggtcgttatg
    17341 gggtaccagg gagctgctgg caactacctg atgtccctta ctatggaaaa agtcgagaaa
    17401 cgcctgacgg atctcagcgg tgctcttgct cataattatc ctgaaatcaa gctaacgaaa
    17461 tatcgccacc agctccagcg tgtcttaacc gccgggctgg taactgaaaa gtgggaataa
    17521 gataagtttt cttgactggg aagtaaaata ccgtatgcgt tcagtcggca aaatttcgcc
    17581 aaatatcctg gatatcttcc atcagatagc cacgatagag cagaaaacgc tggatcttaa
    17641 ctttttctga aaagacagtt ggcaaaggtt catcatattt tcgcgtcgcc tgatcgcgcg
    17701 ccagtgcgca ccagtcgatg tcacattcac gcatcgcttt ttctgtcgct tcgcgggaaa
    17761 tacctttctg attcagttcc tgacgaatac gtgcaggtcc ataacctttg cggctacggc
    17821 tggcgataaa gcgcgcaaca aatcggctgt catcgagata gccatgttca tggcaccatg
    17881 caataacgcg ctcgtaatct tctgccgtag catcaatctc ttctgggcca tttttgccca
    17941 taatcggtgc cgcgagttta cgtcgcagtt cttgctcact gtgatcgcgc accgccagaa
    18001 tgcgtaccgc acgatccaac aggcgagcat atgccgggcg acgggacgtt gattctgtca
    18061 tggcatatcc ttacaactta aaaaagcaaa agggccgcag atgcgaccca ggttttgctg
    18121 ataaacagat ttaatagtca atgagtgtgc agaatgttcc cgcaggccca attcgttaga
    18181 ggatcgaggt gagagtaatt tttcacgcca gtgctggcct gttcccctca ccctaaccct
    18241 ctccccaaag gggcgagggg accgtccgtg cacacattat ctattatcat caataacgaa
    18301 cgattaaaaa tcttcgttag tttctgctac gccttcgctg tcatctacag agaaatccgg
    18361 cgttgagttc gggttgctca gcagcaactc acgtactttc ttctcgatct ctttcgcggt
    18421 ttccgggtta tctttcagcc aggcagttgc attcgcttta ccctgaccga tcttctcacc
    18481 tttgtagctg taccacgcgc ctgctttctc gatcagcttc tcttttacgc ccaggtcaac
    18541 cagttcgccg tagaagttga taccttcgcc gtagaggatc tgaaattcag cctgtttaaa
    18601 cggtgcagcg attttgttct tcaccacttt cacgcgggtt tcgctaccca ccacgttttc
    18661 gccctctttc accgcgccga tacgacggat gtcgagacga acagaggcgt agaatttcag
    18721 cgcgttacca ccggtagtgg tttccgggtt accgaacatc acaccaattt tcatacggat
    18781 ctggttgatg aagatcagca gcgtgttgga ctgcttcagg ttacccgcca gcttacgcat
    18841 cgcctggctc atcatacgtg ccgcaaggcc catgtgagag tcgccgattt cgccttcgat
    18901 ttccgctttc ggcgtcagtg ccgccacgga gtcaacgacg ataacgtcta ctgcaccaga
    18961 acgcgccagg gcgtcacaga tttccagtgc ctgctcgccg gtgtccggct gggagcacag
    19021 caggttgtcg atatcgacgc ccagtttacg tgcgtagatt gggtccagcg cgtgttcagc
    19081 atcgataaac gcacaggttt taccttcacg ctgcgctgcg gcgatcacct gcagcgtcag
    19141 cgtggtttta ccggaagatt ccggtccgta gatttcgacg atacggccca tcggcagacc
    19201 acctgcccca agcgcgatat ccagtgaaag cgaaccggta gagatggttt ccacatccat
    19261 ggaacggtct tcacccaggc gcatgatgga gcctttacca aattgtttct caatctggcc
    19321 cagtgctgcc gccaacgctt tctgtttgtt ttcgtcgata gccattttta ctcctgtcat
    19381 gccgggtaat accggatagt caatatgttc tgttgaagta attatactgt atgttcatac
    19441 agtatcaagt gttttgtaga aattgttgcc acaaggtctg caatgcatac gcagtagcct
    19501 gacgacgcac cgcatcacgg tcgccgctga agcattcccg ccgggtaatg ccttcaccgc
    19561 gggcagtggc aaaagcaaac cagacggtgc cgacaggctt ctcttcacta ccgccatccg
    19621 gcccggcgat accactaata gacacggcat aatcagcacg agccgctttc agtgcgccta
    19681 tcaccatttc caccacgacg ggttcactca ccgcgccatg ctgcgccagc gtctcttcgc
    19741 gtacgccgat catctgcgct ttggcttcgt tactgtaggt gacaaatccg cgttcaaacc
    19801 aggcggagct accggcaata tcggtaatca ctttcgctac ccaaccaccg gtacaagact
    19861 cggcagttgt tacggttgcg ccacgggctt tcagcgcctg cccaacctgt tcacttaact
    19921 gcatcagttc actgtcagtc atcacaacct ctgtcagtca aaaatttatg ccggacaaga
    19981 tagcactttc gtcaggaaga tggtgttggc gttgcaattt tacgaggagg attcagaaaa
    20041 aagctgatta gccagaggga agctcacgcc cccctcttgc aaatagttac tgcacgcgcg
    20101 ccagcgccac ggcttgtcct aactgccaga ccgccattgc gtaatgggtg ctgtggttat
    20161 aacgggtaat ggtgtagaag ttcggcagac cgtaccagta ctggtaaccg gtaccgacat
    20221 ccagacgcag caggctggct tgttgatggt tgcccagcgg ctgctgtggc gttaaacctg
    20281 ccgtggcaag ctgcgaaatg ctgtacctgg ttttgaagcc atttggcaag cctggagcct
    20341 gaccgtttgc cattaccgcg acctgatcgc ctttcaccca gccgtgcgct ttgaaatagt
    20401 tcgccacgct accgatcgca tcaaccggat cccacaggtt gatatgcccg tcgccgctga
    20461 aatctaccgc atattgtttg taagacgacg gcataaactg tccgtagccc atcgccccgg
    20521 caaaggaacc tttcagattg agcggatcgt cctgctcgtc gcgcgccatc agcaggaagg
    20581 tttccagctc gccagagaaa tactccgcgc ggcgtgggta gttaaatgac agcgttgcca
    20641 gcgcatcgag gatgcgagtt ttccccatca cgcgccccca gcgggtttca acgccgataa
    20701 tcccgacgat aatttccggc ggtacgccat acacctgcca cgcgcgattc aacgcatctt
    20761 catactgatt ccagaaaacc acaccgttct gcacgttgtc cggcgtaata aattttttgc
    20821 gataacgcag ccatgcgccg ttcggacctg atggcggttt caccgatgtg gttggtgcct
    20881 ggttatccat cagccgcagt accgaatcca gacgcttcgc ctgggagaga atttcctgca
    20941 actgctgacg atcgaaaccg tgtttgttca ccattttgtc gatgaactgc tgggcattcg
    21001 ggttattagc gaaatcgccg cccatctgca tcacattgtg ctgcggctca agcaggaagc
    21061 cgccagacgg cgttccggtg gtcgtttcag tctcagtagg ttttggcttg ctgctacagg
    21121 cggcaagcaa cacaaaaagg ggaaacaatg ttacataacg acgcttgaac atgaggggtc
    21181 catttaacag attcaaccag gggcaagtat ggtaaagcat cacgccccgc acaaggaagc
    21241 ggtagtcact gcccgatacg gactttacat aactcaactc attcccctcg ctatcctttt
    21301 actcaaactt ccaaattaaa atatttatct ttcattttta gattaaaata acacttttaa
    21361 atctttcaat ctgattagat taggttgccg tttggtaata aaacaataaa tcctgaagga
    21421 gagaacaatg atagaaacca ttactcatgg tgcagagtgg tttatcgggc tgttccaaaa
    21481 gggcggagag gtgtttaccg ggatggtgac cggcattctt ccgctgttga ttagcctgct
    21541 ggttatcatg aacgcactga ttaattttat cggtcagcat cgtattgaac gttttgctca
    21601 acgttgcgcc ggtaaccctg tttcccgtta cctgctgcta ccgtgcattg gcacgtttgt
    21661 cttttgcaat ccgatgacct taagccaggg tcgctttatg ccggaaaagt acaaacccag
    21721 ctactacgcg gcggcctctt atagctgcca ctcaatgaat ggcctctttc cccatatcaa
    21781 ccctggcgaa ctgtttgttt atcttggcat tgccagcggt ctgacaacgc tgaacctgcc
    21841 acttggccca ctggcggtga gctatctgct ggttggtctg gtcaccaact tctttcgcgg
    21901 ctgggtgacc gatctgacca ccgccatttt tgagaaaaag atgggcattc aacttgaaca
    21961 aaaagttcac ctggcaggag caacatcatg acgcgtattc ggatcgaaaa aggaacgggt
    22021 ggctggggcg gcccgcttga gctggaagcc acgccgggca aaaaaatcgt ctatatcacc
    22081 gccggtaccc ggcctgcgat tgttgacaaa ctggcacaac ttactggctg gcaggctatt
    22141 gacggattta aagaaggtga acccgcggag gcggaaattg gtgtcgcggt aatcgactgt
    22201 ggcggcacat tacgctgcgg catctatccg aaacgacgta ttcccaccat taatatccac
    22261 tcgacgggca agtccggtcc gctggcgcag tacattgtgg aagatattta tgtctctggc
    22321 gtaaaagaag aaaacatcac tgtagtgggt gatgcgacac cacaaccctc ttccgtgggc
    22381 cgtgactatg acaccagtaa gaaaatcacc gaacaaagcg atggtttact ggcgaaggtg
    22441 ggaatgggca tggggtccgc cgttgcggtg ctgtttcaat ctggtcgtga caccatcgac
    22501 actgtattaa aaaccattct gccgtttatg gcattcgtct cggcgctcat tggcatcatt
    22561 atggcttctg gccttggtga ctggattgcc cacggtcttg ctccgctggc gagccatcca
    22621 ctgggtctgg tcatgctggc gctcatctgc tccttcccac tgctttcacc tttcctcggc
    22681 ccaggcgcag ttatcgcaca ggttatcggc gtattgattg gcgtgcagat tggtctcggc
    22741 aatattccgc cgcatctggc tttaccggca ctgtttgcca tcaacgcgca ggcggcctgc
    22801 gacttcatcc cggtcggttt gtcgctggcg gaagcccgtc aggacacggt tcgcgtcggt
    22861 gtcccttctg tactggtgag ccgcttttta accggcgcac cgactgtact gatcgcctgg
    22921 tttgtctccg gttttatcta tcaatagagg ctgaaacatg accgttattt ataagaccac
    22981 catcacccgt atcggcgcga gtgccactga cgccctcagc gaccagatgc tcatcacctt
    23041 tcgtgaaggc gcgccagcgg acctcgaaga gtattgcttc attcattgcc acggcgagtt
    23101 gaaaggtgca ctccatcccg gtttgcaatt tacgctcggg cagcatcgct atccggtgac
    23161 cgctgttggc agcgtggcgg aagacaacct tcgcgaactg ggtcatgtca ccctgcgctt
    23221 cgatggttta aacgaagcgg aatttccggg cactgtccat gtggcaggcc ctgtacccga
    23281 cgatatcgcg ccgggatcgg ttttgaagtt tgaatctgtt aaggagtaaa aaatgaatca
    23341 ggttgccgtt gtcatcggtg gtgggcaaac cttaggcgcg ttcctgtgcc acggtctggt
    23401 tgccgagggg tatcgcgtcg cggttgtcga tattcagagc gacaaagccg caaatgtggc
    23461 acaagaaatt aacgccgaat atggtgaaag tatggcgtac ggttttggtg ctgacgccac
    23521 tagcgagcaa agcgttctgg cgctctctcg tggggtagat gaaatctttg gtcgcgtgga
    23581 tttgctggtc tatagcgccg gaatagcaaa agcagccttt atcagcgact tccagctcgg
    23641 cgattttgac cgttcgctac aagtgaatct ggtgggttat ttcctgtgtg cccgtgaatt
    23701 ttcgcgtttg atgatccgcg acgggattca gggtcgcatt attcagatca actcgaaatc
    23761 cggcaaagtg ggcagcaaac acaactctgg ctacagcgca gcgaaatttg gtggcgtcgg
    23821 gctgactcaa tcactggcgc tggatctggt ggagtacggc attacggtgc attcactgat
    23881 gctcggtaac ctgctgaaat cgccgatgtt ccagtcactg ttgccacaat acgcgaccaa
    23941 gctgggtatc aaaccggatc aagtcgagca gtattacatt gacaaagtac cgctcaaacg
    24001 cggctgcgat tatcaagatg tgctgaatat gctgctgttc tacgccagtc ctaaggcgtc
    24061 gtactgcacc ggacagtcga tcaatgtcac cggcggtcag gtgatgttct gatcaacagc
    24121 ggagatccat taaggatctc cgtgagacta tagaatgcct gatgcgctac gctcatcagg
    24181 catacaggac ttccgccact acattaagga aaagttatgg tatccgcact catcaccgtc
    24241 gccgttatcg cctggtgtgc gcaactggcc ttaggcggct ggcaaatttc tcgttttaac
    24301 cgtgccttcg acacactatg ccagcaaggg cgggttggcg tgggtcgttc cagcgggcgc
    24361 tttaaaccgc gggtcgtggt cgccatcggc tggacgatca gcagcgcatc gtcgacacct
    24421 tgtttatgaa aggactgacc gtcttcgccc gaccgcaaaa aattcccgca attaccggta
    24481 tgcatgcggg tgatttacag cccgatgtga tctttcccca tgatccacta tcacagaatg
    24541 ctctatcatt ggcgcttaaa ctgaaacgtg gataatttcg ttgtgaatgt tacttgcttg
    24601 cgaagttatc attttgaaac ctaaatcagg taatcacgcc catgaaacct cgtcagcgtc
    24661 aggccgccat tctggagtat ctgcaaaagc agggtaaatg ctcggttgaa gaattggcgc
    24721 aatactttga caccacaggc acaaccattc gcaaagatct ggtcattctg gaacatgccg
    24781 gaaccgtcat tcgtacttat ggcggagtgg tgttgaataa agaggaatcc gatccgccta
    24841 tcgatcataa aacactcatc aacacccaca agaaagagct gattgcagaa gctgccgtta
    24901 gttttatcca tgatggcgat tcgatcattc ttgatgctgg cagtaccgtt ttgcagatgg
    24961 ttcccctgct ctcgcgcttt aataacatca cggtgatgac caacagcctg catatcgtca
    25021 atgcgctatc cgaactggat aacgaacaaa ctatcctgat gccaggcgga acgtttcgta
    25081 aaaaattggc ctcatttcac ggacagctgg cagagaatgc cttcgaacat ttcagtttcg
    25141 ataaattgtt tatgggcacc gacggcatcg atctcaatgc gggcgtaacc accttcaacg
    25201 aggtttatac cgtcagtaag gcaatgtgca atgccgcgcg cgaagtgatt ttgatggcgg
    25261 actcatcaaa gtttggccgt aaaagcccca acgtagtttg cagtcttgaa agcgtcgata
    25321 agctgattac cgacgcaggt atcgatccgg cgtttcgtca ggcgctggaa gagaaaggga
    25381 tcgatgtgat cataaccgga gagagcaatg agtgaagcac tactgaacgc gggacgtcag
    25441 acgttaatgc tggagttgca ggaagcaagc cgtttaccgg aacgtctggg cgatgatttt
    25501 gttcgcgccg ccaatatcat cctgcactgc gaaggcaaag tggtggtttc gggaattggc
    25561 aaatcgggcc acattggtaa gaaaatcgcc gcaacacttg ccagtaccgg cacaccggct
    25621 ttttttgtcc atccggcaga agcactgcac ggcgatctgg ggatgattga aagccgcgat
    25681 gtgatgctgt ttatctctta ctccggtggc gcgaaggaac tggatctgat tattccgcgt
    25741 ctggaagata aatctatcgc gctgctggcg atgaccggca aaccgacgtc accgctgggc
    25801 ctggcggcaa aagcggtgct ggatatctcc gtagaacgcg aagcctgccc gatgcacctt
    25861 gcgccgacct ccagcaccgt caataccctg atgatgggtg acgcgctggc gatggcggtc
    25921 atgcaggcgc gcggatttaa tgaagaagat tttgcccgct cccacccagc cggggcactg
    25981 ggcgctcgct tgctgaataa agtgcatcat ctgatgcgcc gtgacgatgc catcccacag
    26041 gtggcgttaa ccgccagcgt gatggatgcg atgctggaac tcagccgcac cggtctgggg
    26101 ctggtggcgg tatgtgacgc tcaacaacag gtacaaggcg tctttaccga cggcgattta
    26161 cgtcgctggc tggtaggcgg cggcgcactc accacgccag ttaatgaagc gatgacgaca
    26221 ggcggcacca cgttacaggc gcaaagtcgc gccatcgacg ccaaagagat cctgatgaag
    26281 cgcaaaatca ctgccgcacc ggtggtggat gaaaacggca aactcaccgg cgcaattaac
    26341 ctgcaggatt tctatcaggc cgggattatt taatccttca ttcccagacg tttcgccagc
    26401 cgatgcaggt tggcgacgtc ggtttccagc atccgcgcgc aggcagccca gttatgatga
    26461 ttttgtgcca gtgcctggcg aatagtttca cgctggaacg cttctgtcgc ttcacgcagg
    26521 ttttgcttaa caacgggcac cgccgccgct tctggcggcg gcaacgtcac ctcaggaaaa
    26581 gcaaaatgtt gcgcctcaag aatcacttca tcgccgttgc gggtggctct cgccagcact
    26641 accgcccgat gaatagcatg ttccagttcg cgcacgttcc ccggaaaacg atagtgttgc
    26701 agtaaatttc gcgctccggc acttaatacc acgcgggaga gccccagccg caaacgacac
    26761 tgttcgcaga aataccccgc cagcagaatg acatcatcgc cccgctcacg cagcggcggc
    26821 accgaaagtg gaaacacgct caggcgatga aacaagtcag cgcgaaatcg ccctgccagc
    26881 acctcttcgc gtaagtcgcg gttagtcgcc gccagcacgc gcacatcgac ccgcaaacta
    26941 cggtcatcgc caacgcgctg aatatcgcca tactgcaaca ccctcagcag cttggcctgc
    27001 aatgccaacg acaactcgcc gatctcatcc agaaacagag tgccgttatc cgccatttca
    27061 aacttcccgc tgcggttact gatagcgcca gtaaacgctc ctttcacatg cccgaacaac
    27121 tcactttccg ccacactttc cggcagtgca gcacagttga gatagaccag cggattcacc
    27181 gcccgtggcg aggcttcatg aatcgctttc gccaccagct ccttaccggt tcccgtctca
    27241 ccgctgatca ggacgttgag atcggacgcc gccacaatct caatctcttt tttcagttgc
    27301 gtcatgccag gggacaagcc aatcatctgc gtctgtttca ccgcttcaaa cggcgtggca
    27361 tcgcctggca tcatattctg gctttccagt tgttcaatca gcaacgcatt gcttaacgct
    27421 cccgccgcca gcgcagcaat cagccgtagc tcttcgtcgc tgaaaacatc gaactgatcg
    27481 ggctgcatcc cgtcgagcgt cagtgcgccg atcaggtttt gcccggcaaa caatggcaga
    27541 ccaacgcagg cgtgaacctt cagactctcc tgcccaggaa tcaaaccgtc atagggatcg
    27601 ggcaattcgc tgtctgcggg aaagcgcacc acatccccgg cgcgggcaat cgcttccagc
    27661 cgtggatgcc cttccagcgc aaagcgtcta ccgagtacac cctttgccag accgtcgatg
    27721 gcaagcggaa taaactgccg cgaatcgtaa cgtagcaacg cagacgcatc gcactccagc
    27781 acctgacgta gcgtggtgat caggcgctga aaacgatcct gatgaccaat cccacgctgc
    27841 aattcgatgg cgatattcgc cagcacatca acggaaaaac tcatctttgc ctcactgtca
    27901 atttgactat agatattgtc atatcgacca tttgattgat agtcattttg actactcatt
    27961 aatgagcata attttattta tagagtaaaa acaatcagat aaaaaactgg cacgtaatct
    28021 gcaattagca agacatcttt ttagaacacg ctgaataaat tgaggttgct atgtctattg
    28081 tggtgaaaaa taacattcat tgggttggtc aacgtgactg ggaagtgcgt gattttcacg
    28141 gtacggaata taaaacgctg cgcggcagca gttacaacag ctacctcatc cgcgaagaaa
    28201 aaaacgtgct gatcgacacc gtcgaccata aattcagccg cgaatttgtg cagaacctgc
    28261 gtaatgaaat cgatctggcg gatatcgatt acatcgtgat taaccatgca gaagaggacc
    28321 acgctggggc gctgaccgaa ctgatggcgc aaattcccga tacgccgatt tactgtactg
    28381 ccaacgctat cgactcgata aatggtcatc accatcatcc ggagtggaat tttaatgtgg
    28441 tgaaaactgg tgacacgctg gatatcggca acggcaaaca gctcattttt gtcgaaacgc
    28501 caatgctgca ctggccggac agcatgatga cttacctgac aggcgacgcg gtgctgttca
    28561 gtaacgatgc tttcggtcag cactactgcg acgagcatct gttcaacgat gaagtggatc
    28621 agacggagct tttcgagcag tgccagcgtt actacgccaa tatcctgacg ccgttcagcc
    28681 gcctggtaac accgaaaatt accgagatcc tgggctttaa cttaccagtc gatatgatag
    28741 cgacctctca cggcgtggta tggcgcgata acccgacgca aattgtcgag ctgtacctga
    28801 aatgggcggc tgattatcag gaagacagaa tcaccatttt ctacgacacc atgtcgaata
    28861 acacccgcat gatggctgac gctatcgccc aggggattgc ggaaaccgac ccacgcgtgg
    28921 cggtgaaaat tttcaacgtc gcccgaagcg ataaaaacga aatcctgacc aatgtcttcc
    28981 gctcaaaagg cgtgctggtc ggcacttcga cgatgaataa cgtgatgatg ccgaaaatcg
    29041 ccgggctggt ggaggagatg actggtttac gcttccgtaa caaacgcgcc agtgctttcg
    29101 gctctcacgg ctggagcggc ggtgcggtgg atcgtctttc cacgcgcctg caggatgcgg
    29161 gtttcgaaat gtcgcttagc ctgaaagcga aatggcgacc agaccaggac gctctggagt
    29221 tatgccgtga acacggtcgc gaaatcgccc gtcagtgggc gctcgcgccg ctgccgcaga
    29281 gcacggtgaa taaggtagtc aaagaagaaa cctctgccac cacgacggct gacctcggcc
    29341 cacggatgca gtgcagcgtc tgccagtgga tttacgatcc ggcaaaaggc gagccaatgc
    29401 aggacgttgc gccaggaacg ccgtggagtg aagtcccgga taacttcctc tgcccggaat
    29461 gctccctcgg caaagacgtc tttgaagaac tggcatcgga ggcaaaatga gtaacggcat
    29521 tgtgatcatc ggttcgggct tcgccgcccg ccaactggtg aaaaatattc gcaaacagga
    29581 cgccactatt ccattaaccc tgattgccgc cgacagcatg gatgagtaca acaaacctga
    29641 cctcagccat gttatcagtc aggggcaacg tgccgatgac cttacccgcc agacggcggg
    29701 tgaatttgcc gagcagttta atctgcacct gtttccacaa acctgggtga cggatatcga
    29761 tgccgaagcc cgtgtggtga aaagccagaa taatcagtgg caatacgaca agctagtact
    29821 ggcaaccggt gccagtgcct ttgtcccgcc tgtgcctggg cgtgagttaa tgctgacgtt
    29881 aaatagtcag caagagtatc gcgcctgtga aacgcaactg cgggatgccc gacgcgtgtt
    29941 gattgttggc ggtggtttga ttggtagcga actggcgatg gatttttgtc gtgcaggcaa
    30001 agcggtcacg ctaatcgaca acgctgccag tattctggcg tcgttaatgc caccggaagt
    30061 aagcagccgc ttgcagcatc ggttgacgga gatgggcgtt catctgctgt tgaaatctca
    30121 gttacagggg ctggaaaaaa cggattctgg cattctggca acgctggacc accagcgtag
    30181 tatcgaagtg gatgcggtaa ttgccgccac cggactgcgc ccggaaacag ccctggcacg
    30241 acgcgccggg ctgacgatta atcgcggcgt ttgcgtcgat agttatctgc aaaccagtaa
    30301 tgacgatatt tatgcgctgg gcgattacgc ggaaatcaac ggtcaggtac tgccattcct
    30361 gcagccgatt caacttagcg cgatggtgct ggcaaaaaat cttctcggca ataacacgcc
    30421 gctgaaacta ccgacgatgc tggtgaaaat caaaacgccg gaattaccgc tgcatctggc
    30481 aggcgaaacc cagcgtcagg atttacgctg gcaaattaat accgaacgct agggaatggt
    30541 ggctcgcggt gttgacgatg ctgaccagct tcgcgccttt gtggtcagtg aggatcggat
    30601 gaaagaggca tttggattgt tgaaaacatt gccgatgtag gtgggctact gtgcctaaaa
    30661 tgtcggatgc gacgctagcg cgtcttatcc gacctacggg gacgcatgtg taggccggat
    30721 aaggcgttta cgccgcatcc ggcaatggtg tccaaatgca acacgtttta tccgttctgg
    30781 acttcacccg ctaaccaacg cgccgcagca ataaccccct gccccagaga caaaccgcca
    30841 tcacccgccg gtaaactctg tggaaagagc aatgtgaaat cagcgagata atgcgccaga
    30901 cgtgcacgca gcaaacggtt atgaataacc ccgccgctaa ataccagcgt agtgatacca
    30961 cgcatcgtgg cctgctcacg catcaacgcg gcaaaaccct gcgccagcgc atcatgaaac
    31021 gcccacgcgc gttgattaac ctgtgcctgc cagttcagcc actgctgcca gaaagcggcg
    31081 agatccagtt gattgtccac cagcggcatc gtcaccggat gcgtcactcc gtggcatgag
    31141 gctgcgagcg cctccagagc acaagccgct tcaccttcat aacttaacgt ggctggcgca
    31201 cagcccagtg ccgccgccac tgcatcgaac aaccgcccac acgatgacgc cagcggcgcg
    31261 ttaattccac gctcaatggc ccgcgccagc acgctccagt tttgctgttg cacacttgct
    31321 gtttcagggt aattctgcca ctccggcaca aagcgcaggc actgcgccag caggtttcgc
    31381 cacggctgct tcgctgccaa atcgccaccc ggaagcgcca ctgcaggcaa gccgcccagg
    31441 tgctcacatt cgcgatagtt cacccgcagg cactcgccgc cccacaaagc gccgttctcc
    31501 cccataccga taccgtcgag cgtcaaagca atgacatcac cgccatccag cggccactga
    31561 tgctctgcca gacacgccgc tgcatgggca tgatgatgca gtaccgtttg cgtcggcaga
    31621 ttcatttcac gcgcccactg gctggagaca tagcccggat gcgcgtcatg cacaacgtat
    31681 ttcggggtaa aatcgtagat gttttgcatc aggcgtaacg cttcgcgcca ctgcatctgg
    31741 atgccatcgt cacttaaatc gcccagatgc tgactcaaca ccgcttgttc accgcgcacc
    31801 aggcagaagg tatttttcag atccgctccg agacacagca caggcggaac atttttaaag
    31861 cccggaggca aagccagcgc atccggcaca tacccccgcg aacggcgcag catttcgccg
    31921 ctttcgcgca ccaccgaatc atccatccgc tgcacgatgt cgcggttatg tatcaagaat
    31981 ccgtcggcaa tgccctgcaa atccgccagc gcctgttcgt tgctgatagc tggtggttta
    32041 ccgctcaggt tgccggaggt catcaccagc gggcattgca gttcctgtaa cagcaaatgc
    32101 tggagcgggt tcgcaggcaa cattaccccg acttcgttaa ggtcaggggc gatatcatca
    32161 caaagctcag gaacgtattt tttatccacc agcacaatcg gcgcggcggg cgtggtaagc
    32221 aactggcgcg cagcgtctgg taaaccgtca gccactggca acatgaccgc cagcggtttc
    32281 gccgggcgat gtttgcgcgc ccgaagtgtc gccaccgcgt tactgttacg tgcatcgcag
    32341 gcaagatgaa atccgccaat ccctttgatg gcgacaatgt tgcccatttt taactgtgcg
    32401 atagccgcct gtaatgccgc ctcttgttct gcatgttcac catgacttac ccattcaaga
    32461 tgcgggccac actccgggca ggccaccggc tgggcgtgga agcgacgatc gagcgggtcg
    32521 cggtactctt tgtcacaagc cggacacaac ggaaacgccg ccatcacggt aaacgggcgg
    32581 tcgtaaggca tggcgcgaat aatggtgaaa cgcgggccgc agtgggtaca gttgataaac
    32641 ggataacgat aacgccgttc gcctggggta ttcatttcgg caaggcaagc agggcaagta
    32701 gcggcatccg ggacaatttg cgtattcatg gcacctcccg cgctctggcg gatggtgaac
    32761 tcagtgggca gttgtgacca gatgtacggc tcacgctcga cgctatcaat acgcgccagc
    32821 ggcgggcagt gctgatgcaa ttgaacaaga aacgtttccg ggtcttcccg cagccggact
    32881 tctacgccat cgccgtcatt acagacatcg ccgtgaagat ttaattgctg tgccagctgc
    32941 cagacaaacg gacgaaaacc gacgccctgc actttgccac gaatacgcag ttggacaccg
    33001 caagatgtgt tttttgccat tgagttattc ccgccatcat gaattgcgta acccgcccag
    33061 ccggacacga cagcgtcgca tccggcagtc acaggtcggc gataccgcct gctccgtatt
    33121 ctacgaatat ttccgggaat tcctttgatg ccagaacagt tctgtaagat ttttagaaca
    33181 tcagcgccgt acggcggcgt ttttctgcac tcagttgttc aagtttatta cgatcgacac
    33241 aaatcagcgc atgagtcggg caagccgcca tacacgccgg gccgtcttca cgatggttgc
    33301 acaggtcaca tttattggct tcggctttgt cagcccgtac attcagcccc gcgccgctgt
    33361 tgcgaatcac cggacgtacc accacttcca tcgcgccata cgggcaagcc acaacgcagg
    33421 ttttgcaacc aatgcaacgt tcctgcatca catgaacaaa ccctttatca cggctgatag
    33481 caccattcgg gcagacgtta gcgcacggtg catcttcaca ctgacggcaa actgtcgccg
    33541 tggaaatgtt cacaccttta atgacatgga tacgcggtaa aaatgtttcc ggggtcagcg
    33601 atgcacagtc ctgattttcc tgatgagaaa ccacgcacgc cacttcacag gtacggcaac
    33661 caatacattt actcgcgtca gcaatgatga aacggttcat caaattctcc agcaatgaca
    33721 gttaatgcgc cgatacattc acaaatcatg ccagttttta atttactgtt atttaaggaa
    33781 attaatttct gtaatgcagg aaaaaagatg tcatcgacac tagtgacgat gacatgtgat
    33841 gacaatgttt atcgcgaaag agcaatgagt gagtcgcggt ggatcagttt tccgctgaag
    33901 gttttcggcg gtgagaaatc cccgccatcg agcataaaaa tcagccgtcc aataatttcc
    33961 tgaatcatct cagtcaccgg aatttttacg ctggagagcg ccggaacgat atagggggca
    34021 attgcgatat catcgaatcc gataactgac acctgctctg gcaccgctac gccgcgctcg
    34081 tgcaacgctt tcatcgcacc tatcgccata tcgtcgttac tggcaactaa cgcgctaaat
    34141 ttagccccac gttcgagcaa cgtttctacc ccttcggccc cgctggcggg cgtccattta
    34201 ccgttagcga taagtttttc attgagcgca ataccatgct gcgccagcgc gtctttatac
    34261 ccagcaagac gttcaatgct ggtgggggaa tccatcgagc cggtaaggaa agcaatctcc
    34321 tgatgtccgg cgtttatcaa ctctgccacg gcattaaagc tggtctgttt atgatcgcac
    34381 cagacgctat ggctgctgtt tttgcgcagg cggcgattaa gcaccattat cggctgactg
    34441 tgcgcgtcaa tgatgtcatc gatctcatcc acgcttaaaa aacgcgggta aatcataatg
    34501 gcgttgcagc gcaaatccag cagatactga atcgcctggc gctcttcttc ggcgctgtgt
    34561 ttaccatctg ccaatagcaa ctgccgccct ttctcttccg ccattcgcgc ggcatgaaag
    34621 agtaattcac taaaataaat gccgtggtaa agcgtgttgg tcactaccag ccccagcgtc
    34681 tgagtactct tcgccgacag attgcgcgcc agcaagtttg ggcggtaacc gctctcttct
    34741 accgcctgaa acacgcgatc tttagtctcc tggctgacgt agccattacc tgaaagcacg
    34801 cgggaaacgg tcgcttttga aaccccggcg cgcttcgcca cttccagcat cgtcgtcatc
    34861 attttcatcc ctttacacgc aatcaacgca gtgtactgca ccgtttgccg attgtccttg
    34921 cgcaatcggc gggaaaaata ttcaggtgac cggtttcaca aatataaaaa atgaataatt
    34981 aactctcttg cttatttagt gacaactatt catgattttg tgaaaccggt ttcctaattc
    35041 cgtttcagca tcggcatttt tccgttacgt cgactgataa caactacatc taccctactg
    35101 ataacaggat aaaatccgat ggccaaaaat tatgcggcgc tggcacgctc ggagatagcg
    35161 gcactgggcg gcgttgataa catctcggcg gtcacgcact gtatgacgcg tttgcgcttt
    35221 gttatcaaag atgaccagct tatcgacagc ccgacgttaa aaaccatccc cggcgtgctc
    35281 ggcgtggtac gtagtgacaa ccagtgtcag gtgattatcg gcaataccgt ttcacaagcc
    35341 tttcaggaag tcgtcagcct gctgccggga gatatgcagc ccgcacagcc cgtgggtaaa
    35401 cccaaactca cgccgcgtcg cattggtgcg gggatcctcg atgcgctgat cggcaccatg
    35461 tcaccgctga tcccggcgat tatcggcgga tcgatggtca aactgctggc gatgatcctc
    35521 gagatgagcg gcgtgctgac aaaaggatcg ccgaccttaa ccattctgaa tgtgattggt
    35581 gacggtgctt tcttcttcct gccgctgatg gtcgcggcat ctgccgacat caaatttaaa
    35641 accaatatgt cgctggcgat tgccatcgcg ggtgtgctgg tacatccgag ttttattgaa
    35701 ctgatggcga aagcggccca gggtgaacat gtcgaatttg ccctgattcc ggtcaccgcg
    35761 gtgaaataca cctacacagt gatcccggcg ctggtgatga cctggtgcct gtcatatatc
    35821 gaacgctggg tggatcgcat tacaccggcg gtgaccaaaa acttcctcaa gccaatgctg
    35881 attgtgttga ttgccgcacc gctggcaatc ctgctgattg gcccgattgg tatctggatc
    35941 ggtagcgcca tttcggcgct ggtttacagc attcatggtt atctgggctg gctttcagtc
    36001 gccattatgg gcgcgctgtg gcctctgctg gtaatgaccg ggatgcaccg cgtctttacg
    36061 ccaaccatca ttcagaccat tgccgaaacc ggcaaagaag ggatggtcat gccgtcagag
    36121 atcggcgcta acctgtcgct gggcggttca tcactggcgg tggcgtggaa aacgaaaaac
    36181 ccggaactgc gccagacggc gctggctgcg gcggcatcag ccattatggc ggggatttcc
    36241 gaaccggcgt tatacggcgt ggcgatccgc ctgaaacgtc cgcttatcgc cagtcttatc
    36301 agcggtttta tttgcggcgc ggttgccggt atggcggggc ttgccagcca ctcaatggca
    36361 gcgccggggc tatttaccag cgtgcaattc ttcgatccgg cgaatccaat gagcatcgtc
    36421 tgggtgttcg cggtcatggc gctggcggtg gtgctgtcgt ttatcctcac actgttgctc
    36481 ggctttgagg atattcctgt tgaggaagcg gctgcccagg cgcgaaagca tcagagcgta
    36541 caaccgaccg tcgccaaaga agtaagtctt aattgaggat gaaaatgtca gtatttccag
    36601 aaggtttttt atggggcggc gcgcttgccg ccaaccagtc tgaaggtgcg ttccgtgaag
    36661 gcggaaaagg gctgaccacc gtcgatatga tcccacacgg cgagcatcga atggcggtga
    36721 aactggggct ggaaaaacgt tttcagttgc gcgatgacga gttttatccc agccatgagg
    36781 cgacggattt ttatcatcgt tataaagaag atatcgccct gatggcagag atgggattca
    36841 aggttttccg tacctcaatt gcctggagcc gtctctttcc gcagggcgat gaaatcacgc
    36901 ccaatcagca gggcattgct ttttatcgtt ctgtctttga agagtgtaaa aagtacggta
    36961 tcgaaccgct ggtcacgttg tgccacttcg atgtgccgat gcatctggtc accgaatatg
    37021 gctcctggcg taaccgcaag ctggtggagt ttttcagccg ctacgccaga acctgctttg
    37081 aagcatttga tggtctggtg aaatactggc taaccttcaa tgaaatcaac attatgttgc
    37141 atagcccgtt ctccggcgcg ggtctggtgt ttgaagaagg tgaaaatcag gatcaggtga
    37201 aatatcaggc cgcgcatcac cagctggttg ccagtgcgct agccgccaaa atcgcccatg
    37261 aggttaaccc gcaaaatcag gtgggctgta tgctggcggg cggcaacttc tacccttaca
    37321 gctgcaaacc ggaagatgtc tgggcggcgc tggagaaaga tcgggaaaac ctgtttttta
    37381 tcgatgtgca ggcgcgcggc gcgtatccgg cttactctgc ccgcgtattt cgcgaaaaag
    37441 gggtaaccat agataaagcg ctgggcgatg atgagatcct gaaaaacacc gtcgattttg
    37501 tctctttcag ctattacgcc tcgcgctgcg cctcggcgga gatgaacgcc aacaacagca
    37561 gtgcggcgaa cgtggtgaaa tcgctgcgta acccgtatct gcaagtgagc gactggggct
    37621 ggggaattga tccactcggt ctgcgtatca ccatgaatat gatgtacgat cgttatcaga
    37681 agccgctgtt tctggtggaa aacggcctcg gcgcaaaaga tgaacttgct gccaatggcg
    37741 agattaacga tgactatcgc atcagctatt tacgcgaaca tatccgcgca atgggcgaag
    37801 cgattgcaga tggcattccg ctgatgggct acaccacatg gggctgtatt gatttagttt
    37861 ccgcctctac gggtgaaatg agcaaacgct acggttttgt ctacgtagac cgtgacgacg
    37921 caggcaacgg cacgctgacg cgcacacgta agaaatcgtt ctggtggtat aaaaaagtga
    37981 ttgccagtaa tggggaagat ttagagtaag taacagtgcc ggatgcggcg tgaacgcctt
    38041 atccggccta catgtcgcac gaaccaggaa ggcccgataa gcgcagcgca tcgggcaatt
    38101 tagcatttgg catcagagct ggttcacccc atcaagaaca tccctgtcct gattccttaa
    38161 tgaaaaacta ctcttcttcc accgctaact gcgcgaagcc gccattccct tcccagcctt
    38221 ccagtcgttg ataaacggtt tctaccgcat ctttaatcgg ctgggtcatc gggtagtaaa
    38281 agccgacgat atccggctgt atgccgagga aaatcacttc gccaatatct tctttcaact
    38341 ggtcgataag atagttcaga ggcatgttat gggtagtcat cataaacatc tcggcgatat
    38401 catccgggtc gatgatgcgg atctcgccgg ggtttagccc catatccgtg gcgtcgacaa
    38461 tcagtaaccg cgtagggcgc agttcacgga tggcgacgat gtcgttttcc ggtgcactac
    38521 cgccgtcaat caccacccag ttacctttcg gcgcggcggc gcacttttcc gccagcagcg
    38581 gacctgcgcc atcatcgccc atcatgctat tgccaacaca gagtaaaacg tcagtcacgc
    38641 aatctcctca ccatcaaata gatggcgttt tcctgatgaa tatcatgcag catacttaac
    38701 agcattgtac tccattcctg ctgctgcgaa gtttgcgctg cccgggcttt atcgaatgcg
    38761 ttggccagca tcggcacatg gttaatgtcg atgacgatct caccgtactt cggtacgcct
    38821 tccattttgc ggcgggcttc actgcccgcc tccagagtgg caatccatgc cagatattca
    38881 tcccacgggc aggtgagcgc cgcttccagg caatcgataa ccccaaggtg gtgaccaatc
    38941 gccaggctgt aatagaccac ctgctgcgcc tcggcgggcg tggcatcgtt ctcatcaata
    39001 aatttacggc tcagttgact gaacaccacc ttttcactca tcggatacgc acctcttcta
    39061 caacatgatt cagatggctg acaatctcgt tcagacgcgg atcgttttcc gcttccagcc
    39121 agcgtgcaac ctgttcttcg ccctgcccta actgtgtcag gaagtcatcg gcaatctgac
    39181 gaccgtaacg ataacccgcc aggcggcgtg cttcgcgatc cactttcacg cgcagcggct
    39241 gcaccatatc gccatgcagg atctccgccg gttgttcatc cagttcaccc ggcccacggg
    39301 cgtgaatttt ctgctccagc aggccgagcg ccattgcaaa gccgtacagc gtggcggcag
    39361 gcgttggcgg gcagccaggg atataaacat ccacagggac aattttatcc gtaccgcccc
    39421 acacgcagta gagatcgtgg aagatcccgc cgctgttacc gcaggcaccg taggagatac
    39481 aaattttcgg gtccggcgcg gactgccacg cacgcagcgc aggggatcgc attgcacggg
    39541 tgaccgcgcc ggtaaacagt aaaatatccg catgacgcgg tgaaggaacg actttaatgc
    39601 cgaagcgttc tgcatcaaac agcggcgata aagtggcgaa aatttcgatt tcgcagccgt
    39661 tgcagccgcc gcagtccacg cggtaaacat aggcagaacg tttgattttt ttcagtaacg
    39721 acgccttcat gctggcgatg gattcatcca ccgtcatcgg gaccggaatg ccgttggcgt
    39781 cacgggggcc taataaattg ctcatcagat ggcctctttc atatggcgag tcagttcaat
    39841 acggtcggac ggcaccaggc atttctggcg cttacattcc gggcaagtct caaagctttc
    39901 gcggtggttt tccgcgcggc tgtcgccgtt gtgcttaagc agcgcaatgg cgtagtcgat
    39961 ctctttctgg acggcgaaag gacgattgca gacgcgacag ttgcacagcg cgaagcgaga
    40021 ctgttgcagg aagtcttctt tcttccacac cgccagttcg tactcttgcg acagcttaat
    40081 cgccgccgtc gggcaaactt cttcgcagcg tccacagaag atgcagcgcc cgagattaaa
    40141 ctcccaggcc agctctcctg tggcgaggtc agtttcaacc gttaaggcgt ttgacgggca
    40201 ggcattgacg caggccgcgc agccgatgca ctgctgcggg ttctgctctg gcttaccacg
    40261 gaagttttta tcaaccgcaa tcggctccag cggataagac gaggtcgccg taccggtttt
    40321 gataactttt ttgataaagg taaacatggc gattccttat ttcagcggcg agtttttacg
    40381 ctcaatgctg tagcgctcga gttctttgta cggcaccact ttgctcttct tcttacgtac
    40441 atcgaccaca gtcatgcggt cggtacagga gtagcaaggg tccaggctac cgataatcag
    40501 cggcgcatcg gaaacagtgt tgccacgcag catgtagcgc agggtcggcc agttcgcgta
    40561 ggtcgcggca cggcagcgcc agcggtacag cttctggttg tcgccagtca tgctccagtg
    40621 gatatcatca ccgcgcggcg cttcggcaaa gcccagcgcg aaacggtgcg gaatgtaggt
    40681 aaagccttcc accatcagcg ggccacccgg caggttatcc agaccgtagt cgatcatgtt
    40741 cagcgcggta tagacttcgt tgatacgcac tttcagacgc gaaataacgt cgcagccctg
    40801 ctcgctgtgg acttccattg gcagcaggcc ataaccaaca aacgggtgat cagcgcgggt
    40861 atcgcgagcg tgtccgctgg cgcggaccat cgggccaacg ttactgaaat cgcgggcaat
    40921 ttccgggtcc agacgaccaa tgccgacggt gcgctgttcc atgttcggtg tgctcagcag
    40981 cacatccacc agctcctgca cttcacgacg catctgttgc gccagctggc gggtctggat
    41041 catgtcatct ttcaacagat cgcgacgaat cccgccgatc aggttcaggc cgtaggtttt
    41101 acgcgcaccg gtgaggatct cggccatttt catggaggtt tcacgcacgc ggaagaattg
    41161 cataaagccg gagtcgaagc cggtaaagtg gcaggcgagg ccgaggttga gcagatgcga
    41221 gtgcaggcgt tccacctcca gcagaatggc gcggatcatc tgcgcacgtt ctggcacctg
    41281 aatacccatc gcgttttcca ccgacgtggt gtaggcggtg ctgtgggcga agccgcagat
    41341 cccgcacaca cggtcagaga ggaaggtcac ttcgttataa cccatgcggg tttccgccag
    41401 tttttccata ccgcgatgga catagaacaa gcggtagtct gcgtcgataa tgttttcgcc
    41461 atcgacgaac agacggaagt ggcccggttc gtcagaagtg acgtgcagcg gaccaatcgg
    41521 cacgacgttg tttttcttgt cgcccagttc gttgatgaac tcgtaggttt cagcatcggt
    41581 ggtcggtgcc ggacgctgac gataatccat gctgtcttta cgcagcggat aaagttcatc
    41641 cggccagtca tccggcagca ccagacggcg ttcatccggc agaccaaccg gaatcaaacc
    41701 gtacatatcg cgcacttcgc gctcgcccca caccgccgcc ggaacgcgcg gcgtcacgga
    41761 cggatactcc ggtttgttgg catcaacttc gacgcgaacc gttacccagc acttagtccc
    41821 cttctccatc gacagcacgt aataaacggc gtaatgacca ttcagtttgc gttcgtcgtt
    41881 accaaaaagc accgacagcc agcccccctg cttgtagtag agaaactcca ccacttccgg
    41941 cagatagttc acctttacgg tgacagtcag ctgatctttg gtctgccagg cgtggtccag
    42001 cacgacgccc ggaaatgcct cattcagcgc ggcgagataa tgttgaccta atttttcttc
    42061 agacatgctc aaactctctt taatcacgcc gccagcaagg agacgaacgc taaaaatgca
    42121 aagccaaacc cagcccaggt aatgcgcgga gtaatatcaa gacgcagacg ggccatgctg
    42181 ttttcgaaca gcgcgataac caggacgccg accaccagtt ttacgatcgc aatcaccagc
    42241 gccagcagca gtccaccggc ggtgaaggtt tccatttgtc cccacggaat aaacaccccg
    42301 acgaacatct gcaacaccac cagctgtttc aggctgatac cccatttcat gacgccaaag
    42361 ccgctgccgc tatactcaga gagcggacct tcctgcaact cctgctcggc ttccgccagg
    42421 tcgaacggca gtttgcccat ttcgataaaa gtggcgaacg cacaggcgca gagcgccagc
    42481 accagcggga tgctttggct cagcggccag tgataaacgg tgtcggtaat gttgctgatg
    42541 ttggtggaac cggcaacctg tgcggcaacc cacagaccaa gcagcaacat cggttcgacc
    42601 agcacgccaa gcatcgcttc acggctcgca ccgatagcgg taaacgggct accggtatcc
    42661 agaccggaaa tggcaaagaa gaaacgcgcg atggcgaaga gatacagtaa ggtgatcaaa
    42721 tcgcccagtt gcggcagcgg agaaccgacg gtcaccaccg gcagcgcagt agcgatagtc
    42781 agcatgacgc ccaccatcac atacggcgtc aggcggaaca cccagccaga ggcatccggg
    42841 ccaacgctct ggcggcccag cagtttgata atgtcgcgat actcctgcaa cacgcccggc
    42901 ccgcgacggt tatgcagacg ggcgcgcgcc acgcgggtaa taccggagag cagcggcgca
    42961 acggcaaata acaccagcgc ctgaattaac ggatataaaa cactcattct caggctcctc
    43021 gtgaaacaat aatcaccacc agtaccgcca gttcaaccag cgccatccgg cggaacagca
    43081 acgcactacc ttcgcactgc cagcccggca ccagcgacac cggattcagc catttgcgca
    43141 gtttcagcac tggcgcaaac gcctgtttaa ccggcatggc aaaaccgtga gcggtaatca
    43201 ccattgattt ttcatggtcg tagccgcaca cccaggccgc accgcgggaa cgtgacggca
    43261 ggcggtcgcc tttgcaaatc gccataatga tgaatggcag cagcgggcag gcaatcagca
    43321 gcaatgtgat cattggttga gaaacggtgg tgttagcagg ctccagaggc agaggtacgg
    43381 cagcagagag catcggcagc agccacggcg cagcaacacc gccaattacg cagcaaatcg
    43441 ccagtgccac tacgcttacg ctcatcagga gcggcgcaca ggtggcgttt tcggcttctt
    43501 tggtacgcgg cgcgccgagg aacgtgacgc catagacttt cgccatacac atcaccgcca
    43561 gcgcaccggt aattgccaga cccacagcga gcagcggccc cagcagacgg gcaacaaacg
    43621 cgccactatt gctcagtttg aaaaaggatt gatagataac ccattccccg gcaaacccgt
    43681 tcagcggcgg cagcgcagcc attgccatca acccgactaa catggcgatg gagataaccg
    43741 gcattttctt gccaatacca ccgagttttt cgatatcgcg atgaccggta cggaaccaga
    43801 cgctccccgc cccgaggaac agtacgcttt tgaacaggct atggttaagc agatggtaca
    43861 gaccaccgac caggccaaga gcaatcagcg ccggttgttc gagcgcgata cccgttacgc
    43921 cagcgcccag ccccagcagg ataatgccga tattttccag ggtgtggtaa gccagcaggc
    43981 gctggatgtt gtgctccatc agcgcataca gaccaccgac aaacgcggtg atcatgccga
    44041 gcaccagcag cacgatcccc caccacagcg gtgcattacc gcccagcagt gacagggtta
    44101 aaatgcccag caggccaatt ttcatgacta ccgtagaaaa caacgcggca gctggcgcag
    44161 aggcgttagc atgtgcctgc ggcacccagc cgtgcagcgg aataatcccg gcgagcaggc
    44221 caaagccaat cacaccgagc agccagatat cggaaccgag cggcagctgt tgcatacgca
    44281 tatccagcag gcgcagatcc agcgtgccat aacgctgcca cagcagccag caagcaatcg
    44341 ccagcagcag agtgccaaga cgccccagcg caaaccacag tttgccctct tttgtttcga
    44401 cgtaatactc gatggtgtgg ctgttaaagc catccgccag ggtgacgtaa atacgaaaat
    44461 gttcaaaaag gacgaaacag agttctttgt ccggcgcgca gctcatggcg tgatgcaggc
    44521 gcgctttcga taacgtgatc ccctgaacca gcgaattgca gtagatgtgc cactggtcct
    44581 gtaggcgacg atgccgctgt gcgatgtaat cggctttctc gcttatttcc caaatagtca
    44641 ttgtcaggtt acccgtttaa cagagatgcc agctttaagc attttctgtg ccaattttta
    44701 atttattgtt attaaaacga tttttaagct aaagatgaat ttcgtcgccg tgtcgacgtg
    44761 tcatttcgac atcatcgaca ttattcaccg cagggataat caacactggc acaattattg
    44821 cttgtagctg gcaatagtta atgggaggcg atatgcacga aataaccctc tgccaacggg
    44881 cactggaatt gatcgaacag caggccgcaa aacacggcgc aaaacgcgta actggggtct
    44941 ggctcaaaat tggcgcattt tcttgtgtcg aaaccagctc tcttgccttt tgttttgatc
    45001 tggtttgccg cggcagcgtg gcggaaggtt gtaaactgca cctcgaagaa caagaggtcg
    45061 aatgctggtg tgaaacatgc caacagtatg tgacgctatt gacccagcgc gtccgccgct
    45121 gtccacagtg tcatggtgac atgctacaga ttgtggcaga cgacggttta cagattcggc
    45181 ggatagaaat agaccaggag tgagcgatgt gtacaacatg cggttgcggt gaaggcaacc
    45241 tgtatatcga gggtgatgaa cataaccctc attccgcgtt tcgtagcgcg ccatttgccc
    45301 cggcggcacg cccgaagatg aaaatcaccg gcattaaagc gcctgaattt acccccagcc
    45361 agactgaaga aggcgacctg cattacggtc atggcgaagc gggcactcac gcaccgggca
    45421 tgagccagcg tcggatgctg gaagtcgaaa ttgacgtgct ggacaaaaac aaccgtctgg
    45481 ctgaacgcaa ccgcgcgcgc tttgctgccc gcaagcaact ggtgctcaac ctggtttcca
    45541 gcccaggttc cggtaaaacc accctgctga cggaaaccct aatgcgcctg aaagacagcg
    45601 ttccgtgcgc ggttattgaa ggtgaccagc aaaccgtgaa cgatgccgca cgcattcgcg
    45661 ctaccggcac accagcgatt caggtgaaca caggtaaagg ctgccatctt gacgcacaga
    45721 tgattgccga cgccgcaccg cgtctgccac tggacgataa cggtattctg tttatcgaaa
    45781 acgttggcaa cctcgtatgc ccggccagct tcgatctcgg tgaaaaacac aaagtggcgg
    45841 tgctttccgt taccgaaggt gaagacaaac cgctgaaata tccgcatatg tttgccgccg
    45901 cctcgctgat gctgctcaac aaagttgacc tgttgccgta tctcaacttt gacgttgaga
    45961 agtgcatcgc ctgcgcccgc gaagtcaatc cagaaattga aatcatcctt atttccgcca
    46021 ccagcggtga agggatggac cagtggctga actggctgga gacacagcga tgtgcatagg
    46081 cgttcccggc cagatccgca ccattgacgg caaccaggca aaagtcgacg tctgcggcat
    46141 tcagcgcgat gtcgatttaa cgttagtcgg cagctgcgat gaaaacggtc agccgcgcgt
    46201 gggccagtgg gtactggtac acgttggctt tgccatgagc gtaattactg aagccgaagc
    46261 acgcgacact ctcgacgcct tacaaaacat gtttgacgtt gagccggatg tcggcgcgct
    46321 gttgtatggc gaggaaaaat aatgcgtttt gttgatgaat atcgcgcgcc ggaacaggtg
    46381 atgcagttaa ttgagcatct gcgcgaacgt gcttcacatc tctcttacac cgccgaacgc
    46441 cctctgcgga ttatggaagt gtgcggtggt catacccacg ctatttttaa attcggcctc
    46501 gaccagttac tgcctgaaaa cgttgagttt atccacggtc cgggttgccc tgtgtgcgta
    46561 ctgccgatgg gtagaatcga cacctgcgtg gagattgcca gccatccgga agtcatcttc
    46621 tgtacctttg gcgatgccat gcgcgtacca gggaaacagg gatcgctgtt gcaggcaaaa
    46681 gcacgcggtg ccgatgtgcg catcgtttac tcgccgatgg atgcgttgaa actggcgcag
    46741 gagaatccaa cccgcaaagt ggtgttcttc ggcttaggtt ttgaaaccac catgccgacc
    46801 accgccatca ctctgcaaca ggcgaaagcg cgtgatgtgc agaattttta cttcttctgc
    46861 cagcacatta cgcttatccc gacgctgcgc agtttgctgg aacagccgga taacggtatc
    46921 gacgcgttcc tcgcgccggg ccacgttagt atggttatcg gcactgatgc ctataatttt
    46981 atcgccagcg attttcatcg tccgctggtg gtcgctggtt tcgaacccct tgatctactg
    47041 caaggcgtgg tgatgctggt ggagcagaaa atagcggccc acagcaaggt agagaatcag
    47101 tatcgtcggg tggtaccgga tgccggtaac ctgctggcgc aacaggcgat tgccgatgtg
    47161 ttctgtgtca acggcgacag cgaatggcgc ggcttaggcg tgattgaatc ttctggcgtg
    47221 cacctgacgc cggattatca acgattcgat gccgaagcac atttccgccc ggcaccgcag
    47281 caggtctgcg atgacccgcg cgcgcgttgt ggtgaggtat taacgggcaa atgtaagccg
    47341 catcaatgcc cgctgtttgg taacacctgt aatcctcaaa ccgcatttgg tgcactgatg
    47401 gtctcctccg aaggagcgtg cgccgcgtgg tatcagtatc gtcagcagga gagtgaagcg
    47461 tgaataatat ccaactcgcc cacggcagcg gtggccaggc gatgcagcaa ttaatcaaca
    47521 gcctgtttat ggaagccttt gccaacccgt ggctggcaga gcaggaagac caggcacgtc
    47581 ttgatctggc gcagctggta gcagaaggcg atcgtctggc gttctccacc gacagttacg
    47641 tcattgaccc gctgttcttt cctggcggta atatcggcaa gctggcgatt tgcggcaccg
    47701 cgaatgacgt tgcggtcagt ggcgctattc cgtgctatct ctcctgtggc tttatcctcg
    47761 aagaaggatt gccgatggag acactgaaag ccgtagtgac cagcatggca gaaaccgccc
    47821 gcacggcagg cattgccatc gttactggcg atactaaagt ggtgcagcgc ggcgcggcag
    47881 ataaactgtt tatcaacacc gctggcatgg gcgcaattcc ggcgaatatt cactggggcg
    47941 cacagacgct aaccgcaggc gatgtattgc tggttagcgg tacactcggc gaccacgggg
    48001 cgactatcct taacctgcgt gaacaactgg ggctggatgg tgaactggtc agcgactgcg
    48061 cggtgctgac gccgcttatt cagacgctgc gtgacattcc cggcgtgaaa gcgctgcgtg
    48121 atgccacccg tggtggtgta aacgcggtgg ttcatgagtt cgcggcagcc tgcggttgtg
    48181 gtattgaaat ttctgaatca gcactgccgg ttaaacctgc tgtgcgcggc gtttgcgaac
    48241 tgctgggact ggacgccctt aactttgcca acgaaggcaa actagtaata gctgttgaac
    48301 gcaacgcagc agagcaagtg ctggcagcgt tacattccca tccactgggg aaagacgcgg
    48361 cgctaattgg tgaagtggtg gaacgtaaag gtgttcgtct tgccggtctg tatggtgtga
    48421 aacgaaccct cgatttacca cacgccgaac cgcttccgcg tatatgctaa taaaattcta
    48481 aatctcctat agttagtcaa tgaccttttg caccgctttg cggtgctttc ctggaagaac
    48541 aaaatgtcat atacaccgat gagtgatctc ggacagcaag ggttgttcga catcactcgg
    48601 acactattgc agcagcccga tctggcctcg ctgtgtgagg ctctttcgca actggtaaag
    48661 cgttctgcgc tcgccgacaa cgcggctatt gtgttgtggc aagcgcagac tcaacgtgcg
    48721 tcttattacg catcgcgtga aaaagacacc cccattaaat atgaagacga aactgttctg
    48781 gcacacggtc cggtacgccg cattttgtcg cgccctgata cgctgcattg cagttacgaa
    48841 gaattttgtg aaacctggcc gcagctggtc gctggtgggc tataccccaa atttggtcac
    48901 tattgcctga tgccactggc ggcggaaggg catatttttg gtggctgtga atttattcgt
    48961 tatgacgatc gtccctggag cgaaaaagag ttcaatcgtc tgcaaacatt tacgcagatc
    49021 gtttctgtcg tcaccgaaca aatccagagc cgcgtcgtca acaatgtcga ctatgagttg
    49081 ttatgccggg agcgcgataa cttccgcatc ctggttgcca ttaccaacgc ggtgctttcc
    49141 cgcctggata tggacgaact ggtcagcgaa gtcgccaaag aaatccatta ctatttcgac
    49201 attgacgata tcagtatcgt cttacgcagc caccgtaaaa acaaactcaa catctactcc
    49261 actcactatc ttgataaaca acatcccgcc cacgaacaga gcgaagtcga tgaagccgga
    49321 accctcaccg aacgcgtgtt caaaagtaaa gagatgctgc tgatcaatct ccacgagcgg
    49381 gacgatttag ccccctatga acgcatgttg ttcgacacct ggggcaacca gattcaaacc
    49441 ttgtgcctgt taccgctgat gtctggcgac accatgctgg gcgtgctgaa actggcgcaa
    49501 tgcgaagaga aagtgtttac cactaccaat ctgaatttgc tgcgccagat tgccgaacgt
    49561 gtggcaatcg ctgtcgataa cgccctcgcc tatcaggaaa tccatcgtct gaaagaacgg
    49621 ctggttgatg aaaacctcgc cctgaccgag cagctcaaca atgttgatag tgaatttggc
    49681 gagattattg gccgcagcga agccatgtac agcgtgctta aacaagttga aatggtggcg
    49741 caaagtgaca gtaccgtgct gatcctcggt gaaactggca cgggtaaaga gctgattgcc
    49801 cgcgcgatcc ataatctcag tgggcgtaat aatcgccgca tggtcaaaat gaactgcgcg
    49861 gcgatgcctg ccggattgct ggaaagcgat ctgtttggtc atgagcgtgg ggcttttacc
    49921 ggtgccagcg cccagcgcat tggtcgtttt gaactggcgg ataaaagctc cctgttcctc
    49981 gacgaagtgg gcgatatgcc actggagtta cagccgaagt tgctgcgtgt attgcaggaa
    50041 caggagtttg aacgtctcgg cagcaacaaa atcattcaga cggacgtacg tttaattgcc
    50101 gcgactaacc gcgatctgaa aaaaatggtc gccgaccgag agttccgtag cgatctctat
    50161 taccgcctga acgtattccc gattcacctg ccgccactac gcgagcgtcc ggaagatatt
    50221 ccgctgctgg cgaaagcctt taccttcaaa attgcccgtc gtctggggcg caatatcgac
    50281 agcattccag ccgagacgct gcgcaccttg agcaacatgg agtggccggg taacgtacgc
    50341 gaactggaaa acgtcattga gcgcgcagta ttgctaacac gcggcaacgt gctgcagctg
    50401 tcattgccag atattgcttt accggagcct gaaacgccgc ctgccgcaac ggttgtcgct
    50461 caggagggcg aagatgaata tcagttgatt gttcgcgtgc tgaaagaaac caacggcgtg
    50521 gttgccgggc ctaaaggcgc tgcgcaacgt ctggggctga aacgcacgac cctgctgtca
    50581 cggatgaagc ggctgggaat tgataaatcg gcattgattt aactgcaaat tgccggacag
    50641 atctgcctgt ccggcatact attcacgagg ttttttcgga cgatattttt ccggcagttc
    50701 tggcaccgga cgcttgtcat cgatgagatg acgcacggtt aagatcggat gacgccacag
    50761 cattctcggc ccggcccaac gcataatctg tttcatctct tcacgctttg caggctggta
    50821 acagtgcacc ggacactgct tacaggctgg tttctcttcg ccgaacacac atttatccag
    50881 ccgcttttgc gcgtaaacaa acaacgcctc gtaatgctcc ggctccgctg acgcctgcgg
    50941 gcatttcgct tgataaagat cgatcatttt tttaatcgtc agtttttcac gagagatacg
    51001 cttgccggac atgctgcctc catactctta aggtgtattt atattacaac ttaatttgaa
    51061 aggggactat ggctctgaag gaggaggttt attcaaccga cgcaattttg cgtacttgct
    51121 tcataagcat cacgcaaaag ctgcaaaaca gcatctttcc cggaaccagc atcaagaact
    51181 cgccgttcgc ttcttcccct gaaatgatta actccggtat catgtgcgcc ttatgtgatt
    51241 acaacgaaaa taaaaaccat cacaccccat ttaatatcag ggaaccggac ataaccccat
    51301 gagtgcaata gaaaatttcg acgcccatac gcccatgatg cagcagtatc tcaggctgaa
    51361 agcccagcat cccgagatcc tgctgtttta ccggatgggt gatttttatg aactgtttta
    51421 tgacgacgca aaacgcgcgt cgcaactgct ggatatttca ctgaccaaac gcggtgcttc
    51481 ggcgggagag ccgatcccga tggcggggat tccctaccat gcggtggaaa actatctcgc
    51541 caaactggtg aatcagggag agtccgttgc catctgcgaa caaattggcg atccggcgac
    51601 cagcaaaggt ccggttgagc gcaaagttgt gcgtatcgtt acgccaggca ccatcagcga
    51661 tgaagccctg ttacaggagc gtcaggacaa cctgctggcg gctatctggc aggacagcaa
    51721 aggtttcggc tacgcgacgc tggatatcag ctccgggcgt tttcgcctga gcgaaccggc
    51781 cgaccgcgaa acgatggcgg cagagctgca acgcactaat ccggcggaac tgctgtatgc
    51841 agaagatttc gccgagatgt cgctgattga aggccgtcgc ggcctgcgcc gtcgcccgct
    51901 gtgggagttt gaaatcgaca ccgcgcgcca gcagttgaat ctgcaatttg gcacccgcga
    51961 tctggtcggt tttggcgtcg agaacgcgcc gcgcggactt tgtgctgccg gttgtctgtt
    52021 gcagtatgcg aaagataccc aacgcacgac cctgccgcat attcgttcta tcaccatgga
    52081 acgtgagcag gacagcatca ttatggatgc cgcgacacgt cgtaacctgg aaattactca
    52141 gaacctggcg ggcggtgcgg aaaatacgct ggcttctgtg ctcgactgca ccgtcacgcc
    52201 gatgggtagt cgtatgctga aacgctggct gcatatgcca gtgcgcgata cccgcgtgtt
    52261 gcttgagcgc cagcaaacta ttggcgcatt gcaggatttc accgccgggc tacagccggt
    52321 actgcgtcag gtcggcgacc ttgaacgtat tctggcacgt ctggctttac gaactgctcg
    52381 cccacgcgat ctggcccgta tgcgccacgc tttccagcaa ctgccggagc tgcgtgcaca
    52441 gttagaaact gtcgatagtg caccggtaca ggcgctacgt gagaagatgg gcgagtttgc
    52501 cgagctgcgc gatctgctgg agcgagcaat catcgacaca ccgccggtgc tggtacgcga
    52561 cggtggtgtt atcgcatcgg gctataacga agagctggat gagtggcgcg cgctggctga
    52621 cggcgcgacc gattatctgg agcgtctgga agtccgcgag cgtgaacgta ccggcctgga
    52681 cacgctgaaa gttggcttta atgcggtgca cggctactac attcaaatca gccgtgggca
    52741 aagccatctg gcacccatca actacatgcg tcgccagacg ctgaaaaacg ccgagcgcta
    52801 catcattcca gagctaaaag agtacgaaga taaagttctc acctcaaaag gcaaagcact
    52861 ggcactggaa aaacagcttt atgaagagct gttcgacctg ctgttgccgc atctggaagc
    52921 gttgcaacag agcgcgagcg cgctggcgga actcgacgtg ctggttaacc tggcggaacg
    52981 ggcctatacc ctgaactaca cctgcccgac cttcattgat aaaccgggca ttcgcattac
    53041 cgaaggtcgc catccggtag ttgaacaagt actgaatgag ccatttatcg ctaacccgct
    53101 gaatctgtcg ccgcagcgcc gtatgttgat tattaccggc ccgaacatgg gcggtaaaag
    53161 tacctatatg cgccagaccg cactgattgc gctgatggcc tacattggca gctatgtacc
    53221 ggcacaaaaa gtcgagattg gacctatcga tcgcatcttt acccgcgtag gcgcggcaga
    53281 tgacctggct tccgggcgct caacctttat ggtggagatg actgaaaccg ccaatatttt
    53341 acataacgcc accgaataca gtctggtgtt aatggatgag atcgggcgtg gaacgtccac
    53401 ctacgatggt ctgtcgctgg cgtgggcgtg cgcagaaaat ctggcgaata agattaaggc
    53461 attgacgctg tttgccaccc actatttcga gctgacccag ctaccggaga aaatggaaga
    53521 cgtcgccaac gtgcatctcg atgcgctgga gcacggagac accattgcct ttatgcatag
    53581 cgtgcaggat ggcgcggcga gcaaaagcta cggcctggcg gttgcagctc tggcaggcgt
    53641 gccaaaagag gtgattaagc gcgcacggca aaaactgcgt gagctggaaa gcatttcgcc
    53701 gaacgccgcc gctacgcaag tggatggtac gcaaatgtct ttgctgtcag taccggaaga
    53761 aacctcgcca gcggtcgaag ctctggaaaa cctggacccg gattcactga ctccgcgtca
    53821 ggcgctggaa tggatttatc gcttgaagag cctggtgtaa taacaattcc cgatagtctt
    53881 ttgctatcgg gaatattaac gacaactgac gaataaaata aaaaccccct gtataatagg
    53941 aaagcttatt ttacagggta aaaccatgcc atctacacgc tatcaaaaaa ttaatgccca
    54001 tcactatcgc catatatggg tcgttggtga tatacatggt gaatatcaat tattacaatc
    54061 ccgcttacat caactctctt tttgccctga aaccgactta cttatttctg tcggcgataa
    54121 tattgatcgt gggccggaga gtcttgacgt cctgcgcctg ctaaaccagc cctggtttat
    54181 ctccgttaaa ggcaaccacg aagcaatgga gctggatgcg ttcgcgaccg gcgatggcaa
    54241 tatgtggctt gccagcggtg gtgactggtt tttcgattta aatgattcag agcaacaaga
    54301 ggcaatagat ctgttgctga aattccatca ccttccacat attattgaaa tcactaacga
    54361 cataataaaa tatgtcatcg cacatgcaga ttatccgggt gatgaatatc tctttggcaa
    54421 agaaatagcg gagagcgaat tactctggcc tgtttatcgt gtgcagaaat cgcttaatgg
    54481 cgagttacaa caaataaacg gcgctgatta ttttatattt ggacatatga tgtttgataa
    54541 cattcagacg ttcgctaacc agatttatat tgataccgga tcgccgaaaa gtgggcggct
    54601 gtcattttat aaaataaggt agtctcatgc ttcttctgtg aagcatgagt aaccagatgt
    54661 tattgcaggc cattattcat ttttcgctac cagcaaagag agatcctgct tcgccagcgc
    54721 gcgactggca ctctccggca aaccgtcgtc tgtaataatc tgatcaaact cgcttaatgg
    54781 taacgccagc catgtcgcca cctgaccata tttcgtcgca tcacagacca aaactcgctg
    54841 gcggctggca ctagcaatcg cccgtttcac cgtgacttta tcttccgctg gcgtagaaat
    54901 cccccgcaca ctccatgacg atgcagaaat aaaagcctga tcaatcatca ggctgcgcag
    54961 catggtcgca gcggcttccc cgacacagga acggttttcc cgacacactg caccgccagt
    55021 gtgaataatt gtgcaattac tgttgtcgag caagtagttc gcaataacga aatcgtttgt
    55081 gaccacagtc agtgactcca tgtgaatcag atgctgtgct atcgctaacg tggtcgttcc
    55141 tgcatccaga tagatacaac ttcccggctg aacaagactt gccgccagct tgccaatagc
    55201 cgctttttgc gtcattgcta gcgcagtttt tacctgatga gaaggttcat gcgccacgcg
    55261 tcccggagac tggacgcctc cggacaccag cacaacggct ccctgctgct ccagtttttg
    55321 taaatcccga cgaatggtca tatgtgatac attcattctg tccgttagtt cagcaatact
    55381 gacaatgcct ttttcagcta ccatctcaag gatgatttgg cgacgctcta cgggtatcaa
    55441 cttttgctcc ttcctttgtc ctgctgacat tctacgctat ttgcctgcga aacgtgcgcg
    55501 gcgcaactaa cgcttagttc acataaaata acacacaatg ttaatttatg tgaatcggct
    55561 caccatatcg ttatcttcca gcgcttatat tcacaatatc aaacaaaata tcacttaaat
    55621 taacaaggag agcagatgaa aacgggatct gagtttcatg tcggtatcgt tggcttaggg
    55681 tcaatgggaa tgggagcagc actgtcatgt gtccgcgcag gtctttctac ctggggcgca
    55741 gacctgaaca gcaatgcctg cgctacgttg aaagaggcag gtgcttgcgg ggtttctgat
    55801 aacgccgcga cgtttgccga aaaactggac gctctgctgg tgctggtggt caatgcggcc
    55861 caggttaaac aggtgctgtt tggtgaaaca ggcgttgcac aacatctgaa acccggtacg
    55921 gcagtaatgg tttcttccac tatcgctagt gctgatgcgc aagaaattgc taccgctctg
    55981 gctggattcg atctggaaat gctggatgcg ccagtttctg gtgatgcagt aaaagccgct
    56041 aacggtgaaa tgactgtcat ggcctccggt agcgatattg cctttgaacg actggcaccc
    56101 gtgctggaag ccgttgccgg aaaagtttat cgcataggtg cagaaccggg actaggttcg
    56161 accgtaaaaa ttattcacca gttgttagcg ggcgtacata ttgctgccgg agccgaagcg
    56221 atggcacttg cagcccgtgc ggggatcccg ctggatgtga tgtatgacgt cgtgaccaat
    56281 gccgccggaa attcctggat gttcgaaaac cggatgcgtc atgtggtgga tggcgattac
    56341 accccgcatt cagccgtcga tatttttgtt aaggatcttg gtctggttgc cgatacagcc
    56401 aaagccctgc acttcccgct gccattggcc tcaacagcat tgaatatgtt caccagcgcc
    56461 agtaacgcag gttacgggaa agaagacgat agcgcagtta tcaagatttt ctctggcatc
    56521 actctaccgg gagcgaaatc atgatcaaga ttggcgttat cgccgatgat tttaccggcg
    56581 cgacggatat cgccagtttt ctggtggaaa acggtctacc aacggtacaa attaacggtg
    56641 ttccaacagg taaaatgccg gaagcaatcg acgcactggt gatcagcctg aaaacgcgct
    56701 cctgtccggt ggttgaagcc acacagcaat cgctggcggc tctgagctgg ttgcaacagc
    56761 aaggttgcaa acagatctat ttcaaatact gctctacttt cgacagtacg gcgaaaggta
    56821 atatcggccc ggttaccgat gcattaatgg atgctctcga cacgccgttt acggtcttct
    56881 ctccggccct gtcggtcaac ggacgtacgg tttatcaggg gtatttgttc gtaatgaatc
    56941 aactgctggc cgaatccggg atgcgccatc acccggtaaa tcccatgacc gacagctatc
    57001 ttccccgtct ggttgaagcg caatccacag ggcgctgcgg cgtcgtttcg gcacatgttt
    57061 tcgaacaagg tgtggatgcc gttcgtcaag agcaggctcg cttacagcaa gagggctacc
    57121 gctacgcggt gcttgatgcg ctgaccgaac accatctgga aattcaggga gaagccttgc
    57181 gcgatgcccc actggtaacg ggcggttctg gtctggcgat tggcctggcc cggcagtggg
    57241 cgcaagaaaa cggtaaccag gctcgcgaag cagggcatcc gctcgctggg cgcggcgtag
    57301 tgctctccgg ttcatgctct caaatgacca accgccaggt ggcacattac cgtcaaattg
    57361 caccagcccg tgaagttgat gtggcacgct gcctctcaac cgaaactctg gccgcttatg
    57421 cacacgaact ggcagagtgg gttctgggcc aggaaagtgt acttgctcca ctggtttttg
    57481 ccaccgccag cactgacgca ttggcagcaa ttcaacagca atacggtgca caaaaagcca
    57541 gtcaggcagt agaaactctg ttttctcaac tagcggcgcg gttagcagcg gaaggcgtga
    57601 cacgctttat tgtcgcaggc ggtgagacct ccggcgtagt cacacagagc ctgggcataa
    57661 aagggtttca tattggccca accatttccc ccggcgtgcc gtgggtaaac gcactggata
    57721 agcctgtctc actcgccctt aaatctggca acttcggtga tgaagccttt tttcacgagc
    57781 ccaaagagag tttttatcat gagcgatttc gcaaaagtag agcagtcttt gcgagaggag
    57841 atgacgcgga ttgccagttc attctttcag cgcggctatg caaccggttc ggctggcaat
    57901 ctgtcgctgc ttttacctga cgggaattta ctggcgacac cgacaggttc atgcctgggc
    57961 aatctcgatc cgcagcggct ttccaaagtc gccgcggatg gcgaatggtt aagtggtgac
    58021 aaaccctcga aagaggtgct ctttcatctg gcgctgtatc gcaacaatcc gcgctgtaaa
    58081 gcggtggtgc atttgcacag cacatggtcg acggcgcttt cctgcctgca agggctggac
    58141 agcagcaacg ttattcgtcc gttcacacca tacgtggtga tgcggatggg aaatgtcccg
    58201 ctggtgccct attaccgacc gggcgataaa cgcatcgcac aggatctggc ggaactggca
    58261 gcagacaatc aggctttttt actggcaaat catggcccag tggtttgcgg tgaaagcctg
    58321 caagaagccg ccaacaatat ggaagagctg gaggaaacgg caaagctgat ttttattctc
    58381 ggtgaccgcc cgatccgtat ctgaccgcag gtgaaattgc ggaattaagg agttaatgca
    58441 atgcctcgtt ttgcagctaa tttatccatg atgttcaccg aagtgccttt tattgaacgc
    58501 ttcgccgcag cgcgaaaagc cgggttcgat gctatggaat ttctgtttcc ctatgactac
    58561 tccaccctgc aaatccaaaa gcaactggag caaaaccatc tgacactggc gctgttcaat
    58621 accgctcccg gagatattaa tgcgggggaa tggggattat ccgcccttcc cggacgtgag
    58681 cacgaagcac acgcagacat cgacctggca ctggaatatg cattggcgct caattgtgaa
    58741 caagtccatg tgatggcagg cgtcgtgccc gctggcgaag atgctgaacg gtaccgggca
    58801 gtatttatcg ataatctccg ctacgctgcc gaccgctttg tgccacacgg caagcgaata
    58861 ttagttgaag cactcagccc cgacgttaag cctcattatc tcttctccag ccagtatcag
    58921 gcactggcta ttgttgaaga ggttgcgcga gataacgtgt ttattcaact agatactttt
    58981 catgcgcaaa aagtagatgg caacctgacg catttaatcc gcgactacgc tgggaaatat
    59041 gcgcacgtac aaattgccgg actacctgat cgacatgaac cggacgatgg agaaatcaac
    59101 tatccgtggc tgttccgcct gttcgatgag gtgggatatc agggctggat cggttgtgaa
    59161 tataaacctc gtggcctcac cgaagaaggg cttggctggt ttgacgcctg gcgctaattc
    59221 gttaaatccc taattacaac gtacccatac atccccccat ctacggatgg ggtaggttaa
    59281 ctataacttt cagacagggt ttcccatgtc cacaattaca ttgttatgca ttgcgttagc
    59341 tggcgtaatc atgctgttgc tgctggtcat caaggcaaag gtacaaccat tcgttgctct
    59401 gctcctcgtc agcctgttag tcgcacttgc ggcgggtata ccggcgggcg aagtgggtaa
    59461 agtgatgatc gccgggatgg gcggtgtgct tggttccgtc actattatta ttggtctggg
    59521 cgctatgctg gggcgtatga tcgaacactc tggtggtgca gagtcactgg ctaattattt
    59581 cagtcgcaag ttaggtgaca aacgaactat cgctgcgctg actctggcag cgttcttcct
    59641 cggtattccc gtcttctttg atgtcagctt tattattctt gcgccaatca tttacggttt
    59701 tgccaaggtt gccaaaatat cgccactcaa atttggcctg cctgtcgctg gaatcatgct
    59761 aactgttcat gtggcagtac cgccgcatcc aggccctgtc gccacagcgg ggttactcca
    59821 cgcagacatc ggctggctaa ccatcatcgg tattgcgatt tctattcccg tagggattgt
    59881 tggctacttt gcagcgaaaa taatcaataa gcgccaatat gcgatgtcag tagaagtact
    59941 ggaacagatg caactggctc cggccagtga ggaaggcgcg acaaaattaa gcgataaaat
    60001 aaatccaccg ggcgtcgcgc tggtcacctc gctaattgtt attcctatcg cgattatcat
    60061 gacgggtacg gtttccgcaa cactgatgcc gccttcgcat cccctgcttg gtacgctaca
    60121 gctgatcggc tcaccaatgg tagctctaat gattgcgctg gtgctggcat tctggttatt
    60181 ggctttacgt cgcggctgga gcttacaaca caccagcgac attatgggct cagcccttcc
    60241 cactgcggcg gtagtgattt tggttactgg tgctggaggg gtatttggca aagtgctggt
    60301 ggaatcgggc gtcggcaaag cccttgccaa tatgctacaa atgattgacc tgcctctgtt
    60361 accagccgca tttattattt cactggctct gcgtgcatcg caggggtcag caaccgtagc
    60421 aatcctgaca accggcgggt tactctcaga agcggtgatg ggattgaatc cgattcagtg
    60481 cgtattggtg acgctggcag cctgctttgg tgggcttggc gcgtcacata ttaatgactc
    60541 agggttctgg attgtgacca aatatctggg gttgtcggta gcagacggtc tgaaaacctg
    60601 gacagtgtta acgaccattc tcggttttac cggattctta attacctggt gcgtatgggc
    60661 ggtaatttga ccttaataaa aaggtccgat gggcatcgga ccttttattg tgcacagaaa
    60721 aggccagcct cgcttgagac tggcctttct gacagatgct tacttactcg cggaacagcg
    60781 cttcgatatt cagcccctgc gtttgcagga tttcacgcaa acggcgcagg ccttcaacct
    60841 gaatctggcg aacacgttca cgggtgaggc caatttcacg acctacatct tccagtgttg
    60901 ccgcttcgta ccccagcaaa ccgaatcgac gtgccagcac ttcacgctgt ttggcgttca
    60961 gctcgaacag ccatttgacg atgctctgct tcatatcgtc atcttgcgtg gtatcttccg
    61021 gaccgttctc tttttcatcg gccaggatgt ccagcaacgc tttttcggaa tcaccaccca
    61081 gcggggtgtc taccgaggta atgcgctcgt taagacgaag catacggctg acgtcatcaa
    61141 ctggcttatc cagttgctct gcgatctctt ccgcacttgg ttcatggtcc agcttatggg
    61201 acaactcacg tgcggttcgc aggtaaacgt tcagctcctt tacgatgtga atcggcaaac
    61261 gaatagtacg ggtttggttc ataatcgccc gttcaatcgt ctggcgaatc caccaggttg
    61321 cgtatgttga gaagcggaaa ccacgttccg ggtcaaactt ctctaccgcg cggatcagcc
    61381 ccaggttgcc ctcttcgata aggtccagca acgccagacc acgattgcca taacggcggg
    61441 caatttttac caccagacgc aagttactct cgatcatccg gcggcgagag gcgacatctc
    61501 cacgcagtgc gcgacgcgca aaataaactt cttcttcggc cgttaacagt ggtgaataac
    61561 caatctcacc aaggtaaagc tgagtcgcgt ccaacactcg ctgtgtgact ccctgcgata
    61621 acagttcctc ttcggccaaa tcgttatcac tgggttcctc ttctactaag gccttttcgt
    61681 caaaaacctc aactccgttc tcatcaaatt ccgcatcttc atttaaatca tgaactttca
    61741 gcgtattctg actcataagg tggctcctac ccgtgatccc ttgacggaac attcaagcaa
    61801 aagcctggtt ccgccgattt atcgctgcgg caaataacgc agcgggttta cggatttccc
    61861 cttgtaacga atttcaaaat gcaagcgtgt tgaactggtt ccggtgctac ccatggttgc
    61921 tattttttgc cccgccttca cttcttgttg ttcccggacc agcattgtgt cgttatgggc
    61981 gtaggcactc aggtaatcat cattatgttt gatgataatc agattaccgt agccgcgcag
    62041 cgcgttaccg gcataaacaa cgcggccatc tgcggtcgcg ataattgcct gtcctttgct
    62101 gcctgcgata tcaatcccct tgttgccccc ctcagaagcg ccaaaggttt cgatcacttt
    62161 gccctcagtc ggccagcgcc aggtggagat aggcgtactg gttgatgtac tgctgacagt
    62221 tggctcggtt gtgcttgctg ttggtaccgt tacaggcgct gtgaccgtgg tcgcagctgg
    62281 cttgttgttc ggcaacattt tgttagcact ctgttcaccc gaagattcag aatacgtaat
    62341 tgtcggttgc gacgcaacag caacggtgga attttgtgca gtcttgatca caactccttg
    62401 ctctgctgcg tcggcctggg taatggcatt tccgccagtg attggcgtac cggaagcatt
    62461 acccacctgc aaggtctgac caacgttcag cgcgtatggt gcctgaatat tgttgcgctg
    62521 agcaaggtca cggaaatcgt tgccagtaat ccaggcgata tagaaaagtg tgtcgccttt
    62581 tttcacggta taggtactgc cgctataact gcctttcgga atgttcccat actgacggtt
    62641 atagacgatg cgtccgtttt ccatctgtac cggctgctga gctactggct gcactggctg
    62701 gatttgcggt tgctgggctg tagacgtcgt ccccattttc ggcggcggcg taatcaacat
    62761 accagaattg gtatttgcag gcgcattgcc attaacggag ctgaccggtg ccggtggatt
    62821 tgaagtgtca gaacagcctg ccagccatag cgaaaccagt gacaaagccg caatgcggcg
    62881 aacggtgaat tttgggcttc ccgcgctcat ttatccccca ggaaaaattg gttaataacc
    62941 agtgacataa ttaccgtgca aggcacccta ctgaacactg gaaaagatgt tcacgatacg
    63001 ctgacctgcg gcaaaataac caggaaaaat ccaggtattt cctcacgttt taagccagct
    63061 cccccttcac taaagggaca aagcgcacgg cctccacggt atcgataata aattcgcctc
    63121 cccgacgacg cacccgtttc aaatactggt gctcctcccc tacgggtaag acgagaatcc
    63181 cgccttcgtc cagctgcgtc attagcgcag ttggaatttc cggcggtgcc gccgtaacaa
    63241 tgatagcgtc aaacggcgcg cgtgcctgcc aaccttgcca tccatcgcca tgacgggttg
    63301 aaacattatg taaatcaaga tttttcaggc ggcgacgcgc ctgccactgc aaacctttaa
    63361 tccgttcgac cgagcaaaca tgctggacaa gatgcgccag gattgccgtt tgatatcccg
    63421 aaccggtgcc aatttccagc acccgcgact gcggcgtcag ctcgagtaat tcggtcattc
    63481 gcgccaccat atacggctgc gaaattgtct gcccctgacc tatcggcaag gcgatattgt
    63541 cccaggcttt ttgttcaaac gcttcatcaa cgaatttttc acgcggcacg gcagcaagtg
    63601 cattcagcac ctgctcatcc tgaatacctt gcgcacgtaa ttgatccaga agtgcttgta
    63661 cgcgtctgct taccattgcg tgccaactcc cacgctgttt aaccagtctg aaaccacatc
    63721 ttgcgcgcta tgcgcagtta aatccacatg cagcggcgtg atggagacat agccctcatc
    63781 taccgcagca aaatcggtcc ccggaccggc atcacattta ccgcccggcg ggccaatcca
    63841 gtacagcgta ttgccgcgcg gatcttgctg cgggatcacc tgatctgccg gatgtcgtgt
    63901 accgcagcgc gtcacgcgaa tacctttgat ttgatccaag ggtaaatccg gaacgttaat
    63961 attaagaata cgtccggtgc gcagcggctc tttacacagt gcgcgcaaaa ttgaacaggt
    64021 taccgccgcg gcagtgtcgt aatgtttatg cccgtcaagc gagacggcca gcgccggaaa
    64081 acctaaatga cggccttcca tcgcggcggc taccgtaccg gaataaataa cgtcatcccc
    64141 cagattcggc ccggcgttaa tgccggacac aacaatgtcc gggcgcggac gcatcagagc
    64201 attcacgcca agatagacgc aatcggtcgg ggttcccatt tgcacagcaa tatcaccatt
    64261 ttcaaaggta aacgtgcgca gggaggattc cagtgtcaga gaatttgaag cgccgctgcg
    64321 gttacgatcg ggggcgacca cctgaacgtc agcaaactca cgcaaggctt tcgccagcgt
    64381 ttgtataccg ggtgcatgta ccccgtcatc attactcagc aatatgcgca taatcacctg
    64441 ttgtgttgat aagttccctg acaacgctgg ttgcaaaact acccgccgga agccagaaac
    64501 ggatctctac ggtgacgtca tcccaccaat tccagcttaa ttgttgcgga tacagcagca
    64561 tcgctctgcg cgcggcttca actttttcgc gcaccagtaa agcttgtaat tcagtttctg
    64621 cggcgacagc tgcttgttcg aatgccagcg ctttacgctg agttccccat tcgccactgc
    64681 ccggcaatgc ggcggttatc atcagctctt tatcgttgac gcgatgctgt aattccgcca
    64741 gttcttcggt ggttgctaca aaccagctac cacgtccggc taattgtagc gcatcgccgt
    64801 caacaacttg attaacgtct gcttttttga ggcgctcagc aacaatctga ttaaacaacg
    64861 cactgcgggc tgccgacaac caaaaactcc gtttattgcg atcgcgcacc ggagtattgg
    64921 tttgcgccca gcgcagcgcg ccctgcaagt tgctaccgcc aatcccaaaa cgttgggcac
    64981 cgaagtagtt cggtacacct tttacgcaaa tatcgatcag acgttgttca acgtcatcgc
    65041 gattgctcac ttcgcgcaga accagggtaa aggcgttacc tttcagcgcg cctaaacgca
    65101 gcttgcgctt gtgccgcgca tactccagca cctggcagcc ttccagttga aaggcgctca
    65161 gatcgggcat ttccttgcct ggcacgcgag cgcataacca ctgttccgta acagcatgtt
    65221 tgtctttttg cccagcgaag ctgacttcac gggcatgaat tttcaggaat ttcgccagtg
    65281 catccgccac aaaacgggta ttgcagccgt ttttgaggat tctaaccaga atatgctcac
    65341 cttcaccatc aggctcaaag cccaaatctt ccaccaccac aaagtcttcc ggattggctt
    65401 tcagcagccc ggtgccttgc ggtttaccgt ggaggtaagt gagattatca aactcaatca
    65461 ttttgttgcc ttaatgagta gcgccaccgc ttcacaggca atcccttccc cacgtccggt
    65521 aaatccaagt ttttccgtag tagtggcttt cacgttaaca tcatccatat ggcagccgag
    65581 atcttcggca ataaacacgc gcatttgtgg aatgtgcggc aacatcttcg gtgcctgagc
    65641 gatgatagtg acatcgacgt tgcccagggt ataacccttc gcctgaatac gacgccaggc
    65701 ttcgcgtagc agctcgcggc tgtcggcacc tttaaatgcc ggatcggtat ccgggaacag
    65761 cttgccgata tcccccagcg ccgccgcgcc aagcaatgca tcggtcaacg catggagcgc
    65821 cacgtcgcca tcagaatgcg ccagcaatcc tttttcgtaa ggaatgcgta cgccaccaat
    65881 gataattggg ccttcaccgc caaaggcatg tacgtcaaaa ccgtgtccaa ttcgcattat
    65941 gtattctcct gatggatggt tcgggtgagg taaaactcgg ccagtgccaa atcttccggg
    66001 cgcgtgactt taatgttatc cgcacggcct tcgaccaact gaggatggaa tccgcaatat
    66061 tccagcgccg aggcttcgtc ggtaatagtc gcgccttcat ttagagcgcg cgtcagacag
    66121 tcatgtaaca gctcacgagg gaaaaattgc ggcgtcagcg cgtgccataa gccgttgcga
    66181 tcaacggtat gagcaatggc atttttgccc ggttcggcac gtttcatcgt atcgcgcact
    66241 ggtgcggcta ggatccctcc cgtgcggctg gtttcgctca acgccaacaa tcgcgcgagg
    66301 tcatcctgat gcagacaagg acgagcggcg tcatgcacca atacccactg cgcgtcgcca
    66361 gcggctttca aacctgccag cacggaatcg gcacgctcat caccgccatc tacaacggtg
    66421 atttgcggat gattcgccag aggaagttgt gcaaaacggc tatcgccagg acttatggca
    66481 atgacgacac gtttcacccg gggatgcgcc agcagcgcat gcaccgagtg ttcaagaatg
    66541 gtttgattac cgattgagag atattgctta ggacattccg tttgcattcg acggccaaat
    66601 ccggccgccg gaaccacggc gcaaacatcc aaatgagtgg ttgccatgtt aattcccggg
    66661 ctgatttatc gattgttttg ccccgcagac tgtgcgcgct tcgacgcgtc aggcaccaga
    66721 cgataaaaag tttcgcccgg cctggtcatg ctgagttcat tacgcgcacg ctcttcgagc
    66781 gcctcctggc cgccattgag atcgtcaatt tcggcaaaaa gttgatcgtt tcgcgcttta
    66841 agtttcgcgt ttgtagcttg ctgtgccgcc acatcatcat tgacgcgggt atagtcatgt
    66901 ataccgttct taccgaacca cagcgaatac tgtagccaga ccagaatagc cagcaacagc
    66961 agcgttagtt tacccatcct gccccctgaa aaacggcatc atcatcccat gcatccgaag
    67021 acgactctac atcctctgtt ggggataccg cgacaacgcg ggcaaatgta ccacatttgt
    67081 ccattgttac gtatacctag ggcgtgcaga acataatctc attattagtt acggtttgaa
    67141 ttatgaacag aggagacaag aaagtacaaa ttagcccagt agccacataa acagtgcgcc
    67201 aaacataatg cctactgtca ccagggtgaa aacaatactg tagcgtagct ttccgtccat
    67261 caatgaatgc agcgcaatcc ccaccactac cgcgacaggc atcagtgcca gaaagaaagg
    67321 ccaggtgtag ataaagaaga acagcgtgtt agagccataa atcaacatcg gcatcgccag
    67381 cgcaaataac caggagataa aaccgaccac ggcaccaggt agtgaccatg tggtttcttc
    67441 atcctcagta aggctgtcgt tatttgttag tgtaatgtta tggctattac gcatatttga
    67501 tcctgttact ttgacgaacc gggcatggaa acccggtggt gtctcaggat ctgataatat
    67561 cgttctgtct caacagatct aataattgct gtaccaaatt tgttactaat tgttcaccat
    67621 tgagatgaat ttctgccgat tcaggcgctt cgtaaacgga atctattccc gtaaagttgc
    67681 gcagttcacc ggcacgcgct ttcttatata agcctttggg atcgcgggct tcgcaaatcg
    67741 ccagcggcgt atcgacaaac acttcgataa agcgcccttc tcctacgcgt tcgcgaacca
    67801 tctggcgttc ggcgcggtgt ggcgagataa atgcggtcag caccaccagt ccggcttcaa
    67861 ccatcaaatt cgccacttca ccgacgcgac ggatattctc tttacgatcg gcatcgctaa
    67921 aaccgagatc gctgcataat ccgtggcgaa cattgtcgcc atccagcaga tacgtactga
    67981 cgccgagttt atgtaacgcc tcctccagcg ccccggcgac cgttgattta ccggacccag
    68041 agaggccggt aaaccacagc actacaccac gatgaccgtg gtgtagctcg cgttgttgca
    68101 cagtgaccgg atggctatgc cagacgacgt tttcgtcatg cagcgccatt atttatcccc
    68161 cagcaaatcg cgcgcgcccc agtgcggaaa gtggcgacga accagggcat tcaattccag
    68221 ttcgaatgca ctgaattcag atgacgcaac ggcggcctcg tttttcggct catgcaccat
    68281 accggcacca acagtcacgt tgctcaggcg atcgataaaa atcagcccac ccgtcaccgg
    68341 attttgttga taacgatcta acaccagcgg ctcgtcaaaa gtgagatcca cgaggccgat
    68401 cccattcagt ggcaggtttt caacttcacg ctgggtaagg ttattaatat caaccggata
    68461 gcgaatgcca tcaacacgcg cgcgcgtctt cttaccggca attttgatgt cgtaactctg
    68521 gcccggggaa agcggctgtt ccgccatcca taccacatcc accgacgcgc tcggcaccgc
    68581 cggtaacgct tcgtctgccg ccagcagcag atcgccacgg ctgatgtcga tctcatccgt
    68641 cagcaccagg gtgatcgctt ctccggcaaa ggcttcttcg cgatcaccat caaaagtcac
    68701 gatccgcgcg acgtttgatt ccacaccaga gggcagcgct ttgacacgtt gcccgacttc
    68761 cacgcgaccg gatgccagcg ttccggcgta gccacgaaaa tcgagattcg ggcgattaac
    68821 gtactgcacc gggaagcgca ttggctgggc atccaccact cgctggatct ccacggtttc
    68881 cagcacttcg agcagtgtcg gaccgctgta ccacgacata ctttcacttt gcgatgccac
    68941 gttgtcgcct tccagtgcag agagcggcac aaagcggata tccagattac ccggcagctg
    69001 cccggcaaag gtcaaataat cttcacgaat atgggtgaac gtctcttcac tgtaatccac
    69061 cagatccatt ttgttgatcg ccacgaccag atgtttgatc cccaacagtg tggagataaa
    69121 actgtgacga cgggtttgat cgagcacgcc tttacgggca tcgatcagta agatcgccag
    69181 ttcacatgtc gatgcgccag ttgccatatt gcgagtgtac tgctcgtgcc ctggggtgtc
    69241 ggcgataata aatttacgct tctcggtaga gaaataacgg taagccacgt caatggtgat
    69301 gccctgttcg cgctcagctt gcaggccgtc caccagcagt gccagatcca gcttttcgcc
    69361 ctgggtgccg tgacgcttac tgtcgttatg cagcgatgag agctgatctt cgtagatttg
    69421 gcgggtatcg tgcagcaggc gaccaatcag ggtacttttg ccgtcatcga cgctaccaca
    69481 ggtcagaaaa cgcagcaggc ttttatgttg ttgcgcaatc atccaggctt cgacgccgcc
    69541 ttcattggcg atttgttgtg caagtgcggt gttcatctta aaaatacccc tgacgttttt
    69601 tcagctccat agaccccgcc tggtcgcggt caatcacgcg gccctgacgt tcactggtgg
    69661 tagaaaccag catctcttcg atgatctccg gcagtgtttg tgcatttgac tccaccgcac
    69721 cggtcagcgg ccagcagccc agcgtacgga aacgcaccat ccgtttttta atcacttcgc
    69781 ccggttgcag gtcgatacgg ttgtcatcaa tcatcatcaa cataccgtcg cgttccagaa
    69841 ccggacgttc cgcagcgaga tacagtggca caatgtcgat attttccagc cagatgtatt
    69901 gccagatatc ctgctcggtc cagttagaga gcgggaagac gcggatgctt tcacctttgt
    69961 taatttgccc gttgtagttg tgccacagct ccgggcgctg atttttcgga tcccagcgat
    70021 ggaagcggtc acggaaagag tagattcgct ctttagcgcg ggatttctct tcatcacggc
    70081 gcgcaccacc gaaggcggca tcaaaaccgt atttgttcag cgcctgtttc aggccttcag
    70141 ttttcataat atcggtatgt ttcgcgctgc cgtgtacgaa tggattaatc cccatcgcca
    70201 cgccttccgg gtttttatgc accagcagct cgcagccgta ggctttagca gtacgatcgc
    70261 ggaactcata catctcgcgg aatttccagc cggtatcgac atgcagcaac gggaaaggca
    70321 gcgtacctgg ataaaacgcc ttgcgcgcca gatgcagcat gacgctggaa tctttaccga
    70381 tagagtagag catcaccgga tttgagaatt ctgccgccac ctcgcgaata atgtggatgc
    70441 tttccgcctc cagttgccgc aggtgagtaa gtcgtttttg agccataacc gttcctttgc
    70501 aataccgcta ttttcttgcc atcagatgtt tcgactatag ggagcgtaag agaacgaatg
    70561 aaattaccaa ttagaatgag tagttcctta acggaataac gatttggcaa agctaatatc
    70621 aaaaagtgct taaggcaccg gatttcgggc gtttaggaag atttgaaatt gttttagcgc
    70681 agcggcagtt tcatactatg gcggtaaaaa aatttgcatg gtatttaagg actcactatg
    70741 ttttccgcat tgcgccaccg taccgctgcc ctggcgctcg gcgtatgctt tattctcccc
    70801 gtacacgcct cgtcacctaa acctggcgat tttgctaata ctcaggcacg acatattgct
    70861 actttctttc cgggacgcat gaccggaact cctgcagaaa tgttatctgc cgattatatt
    70921 cgccaacagt ttcagcaaat gggttatcgc agtgatattc ggacatttaa tagtcggtat
    70981 atttataccg cccgcgataa tcgtaagagc tggcataacg tgacgggaag tacggtgatt
    71041 gccgctcatg aaggcaaagc gccgcagcag atcatcatta tggcgcatct ggatacttac
    71101 gccccgctga gcgatgctga cgccgatgcc aatctcggcg ggctgacgtt acaaggaatg
    71161 gatgataacg ccgcaggttt aggtgtcatg ctggaattgg caaaacgcct gaaaaatacg
    71221 cctaccgagt atggtattcg atttgtggcg accagcggcg aagaggaagg gaaattaggc
    71281 gctgagaatt tactcaagcg gatgagtgac accgaaaaga aaaatacgct gctggtgatt
    71341 aatctcgata acttaattgt tggcgataaa ttgtatttca acagcggtgt aaaaacccct
    71401 gaagcagtaa ggaaattaac gcgcgacagg gcgctggcaa ttgcgcgcag tcacggaata
    71461 gccgcaacga ccaatccggg gttgaataaa aattatccga aaggcactgg gtgttgtaat
    71521 gacgcagaaa tattcgacaa agcgggcatt gctgtacttt cggtggaagc gactaactgg
    71581 aatcttggga ataaggatgg ttatcagcaa cgcgcaaaaa cagccgcatt ccctgcggga
    71641 aatagctggc atgacgtaag actggataat caacagcata ttgataaggc acttcctgga
    71701 agaatagaac gtcgctgccg tgacgttatg cggataatgc taccgttggt gaaggagctg
    71761 gcgaaggcgt cttgatgggt ttgaaaatgg gagctgggag ttctaccgca ggggcgggga
    71821 attctaagtg atatccatta tcgcatccag tgcgcccggt ttatccccgc tggcgcgggg
    71881 aacacactaa gcatacataa ctgtttttaa acaaatttat tccgcatcaa caatctacca
    71941 actaaattaa aacatttcct tatttttaaa gaacggataa cttattgatt atcaacagga
    72001 agaaaagaaa ccaaacgtaa cccatccaaa tccaccggaa tacgtctgtt ttctccccag
    72061 gtctgaaatt caaaacccga ctcggtattg gtcgcccagg ccatcaccac atttccgcaa
    72121 ccagccagtt gggtaatttg ctgccagatc atctcccgaa tacgttttga tgtatcacca
    72181 acatacacac cggcacgcac ttccagtagc cagattgcga gccgtccacg taagcgtggc
    72241 gggacatttt ctgtaacaac cacgaccatg ctcatcagcc gcgtcctcga tgaccgctat
    72301 cgcccaacgt ttcaggttcg gggatggctg gcggtaacat atccggcgca ggttgtggcg
    72361 gttcaatttc acctgcggca aggacttcct caattaacgg tattaatttg cccgttaact
    72421 tagtgctacg gaaaatatca cgacaggcta atctgacttc tttatcaggt tctgcgggtt
    72481 gcctcgccgc tatttcaaat gcctttggta caaccgaatc aaatttaatg atatcggcta
    72541 tgtcataaac aaatgaaagc ggtttgccac tatggataaa cccaatagcg ggcgcatatc
    72601 ccgcggctaa tactgccgct tcagatatac cgtacagaca tgatgtagca gcactgatgc
    72661 agcgattcac aacatcgcct ttttcccagt ctttaggatc gtatttgcga ccattccatt
    72721 tcacaccata ttgtttcgcc agtaatgcat acgtctggcg aacgcgagat ccctcaattc
    72781 cacgtagctg ctccactgaa cggcgagctg gcggtggctc acgaaaacgt aattcataca
    72841 ttttgcgcac caccttcaga cgtagatctt ccgttaaagc cagctttgcc tggtagagta
    72901 acttatcagc acgcgcccca ccgggttgtc cggaagagta gacgcgaaca cccgcttcac
    72961 cgacccagac ctgtagcgtc cccaccgtgg cggccagatg caccgccgcg tgggaaactc
    73021 tcgttcccgg ttcgagcata atgcaggcga ccgatcccac cggaatgtgc gtgcggatcc
    73081 cggttttatc gatcagcacg aaagcgccgt ccagtacgtc gatttgcccg tactggagga
    73141 agatcataga ggtgcgatct tttaacggga tcgggctcag cggtacaaac gtcacgcctc
    73201 tgctccgggt ttgatcagca tcagaccaca gccgaacgcg cggcttttgc catacccctg
    73261 actgagacgt tgcagaaata acccgggatc ggtgaccgta agcatccccg tataatccac
    73321 gctactgaac tggatcagtt gctgggagtt ttcccgccgc agttgctgtt gtcggtaggc
    73381 atcaaccgag atatccagca gtgtaaaacc acttctctcc ccctgcgctg ccagccagtc
    73441 cagcgccgcc tgttgttgat gcaaccagac atcacttcct tccgcctgcc ccctcacctg
    73501 ccgtttcgcc tccatcagca gatcgtggcg cttgcccgct ttacagattg ttggattcgc
    73561 ccgcaggttg aaacaaagtt gttgtccggt acacagctcg ggcgcaaatg accggcattc
    73621 tatggtgaac gtatcacttt ccgccgggcg ttcctgcgac agtacaaaaa agcgaaacgc
    73681 gccctggagt tcttcccgac gataaagaaa ttgcctttct ttgccgctag ggaagagatc
    73741 ccacagccac tgatgcatca catattcacc gcgatccacc agatgcagca actgcgcagg
    73801 cgaaagctgg ccggtatgta aggttattct tgagaggtac atggttcctc cttgctgagc
    73861 cacggcccct gattgacggt gcgttcccca aacagccact gctgacgatt taaaggaaca
    73921 tcccggcggc gtaatatttt gctcgcgacc aggccgtcgt gtttcccttc ccaccagcat
    73981 tcatcctgaa gtttcgggag tgaaactttc agtttatgga aatgatcctg atactgttgg
    74041 tatgcattac ataaggcatc acacgcgttg ccttcgagca gtaacggcgc aagaggtaat
    74101 gccagcggat gactttttcg ccccagataa agcggaaaaa ctggatggcg taaaccgtcc
    74161 tgcaactgtt cgaggctgta aggcgcatcg gctgttgttg ccaccgccac catccaccag
    74221 gcatcggtgt agtagtcgcg ccgggagata atcgcgctca aaagctcagg gtcgctcaac
    74281 tcttcgcgac ggctgaaata acgtgcttta cgcacctctt ttggcatctg gaccgtgtga
    74341 taatcccgtg cccagcgcgg gttacggctg gcgcaaacca ccagtgaata gtggcgatta
    74401 aacgcgttta agcgttcggt atcatcacgc cggatcccta ccccggcagc cagcagcccc
    74461 agcaatgctg agagcgaagg cagttcatgg gtatgacgca cttcgccggg ggcatcgacg
    74521 ccccaggatg ccatcggccc atgaagctga aaaatcagat attggctcat aagccgcccc
    74581 ttacgcgcag ataaagtcca gcacgtcctt catgcttccc tgtttgttca tcacgtcaaa
    74641 acttgcgtaa tcggtcttct gttcgtagac cgtattcata ttttcgcgaa gagttgtaat
    74701 acgctgcacc gccacctcta actgacgggt gccattaatg ggttcataga aagctgccgc
    74761 cagagaacgc ggttgatcgg tgcctttttc tgccagcgcc caggaggcat aagcccggct
    74821 ggcaaagctg ttttgtttac cggttgggga gactttaagc gcggcttccg taaaggcgcg
    74881 tatggtctga ttagctaacg cttcgtcacc gccgaggttt tctaccagca gatctttatc
    74941 aatgcagata agatataggt gtagaacagc gcagaaccga atccggtttc atctacatga
    75001 ccggcaccgg catcttcaga agcctggcgt aaatcatcta cggcggtgaa aaaatcattt
    75061 tcgacaatcg tttcactgac accaaacgca tgcgcgacct ggcaggcggc ttctacatta
    75121 aacccgggtt ttttcgccag catacgacca aacatagcga tatctaccgc catacggtct
    75181 ttacgtaaca aagcgagatc tttctctttt ggcgcgcgct tttcttctgc cagttgatgg
    75241 gccagcgctt ttacggcgtc aaattctgcc gggctgatat ggacgagctg ttcagtttcg
    75301 gcgctagtga gcggatcttt tggttttttg tcgttttttt gttttttgtc ttttttggct
    75361 ttcccaagat aatcggctat ctctactgcc cattcgatgg cttttttatc ttcgattcct
    75421 ttctcaatca ggatagttgc cgcctcacgc gcaatacgcc cgctacgaac accaatatgg
    75481 cccgccagcg cctgttcaaa aagttcagaa gtgcgccaag cacgcttcag actttgcgag
    75541 gaaacgcgca gacgcgttgc tccacccaga atcacagttt tcggagagcc ggaatcatca
    75601 cggttaagat tggcagcagg gtaagcggtt aacaaatgaa gctgaataaa tgttgtcatg
    75661 agatagtcct ttattaattt tgttcgttat cggcgtcgcc agcctggtaa tattccagcg
    75721 cccagcgccc agcgcccagc gcccagcgcc cagcgcccag cggatacgga taaattctgt
    75781 tggtcgctgc tggcggcggt gatgattgag caggtcatcg ctctcctggc accagcggaa
    75841 gaccccctct tctagagagt caagattgac ggaaccgttt aataatttga ccgcacggcg
    75901 taactgccgc aataaatcat ccggcgtttt tactgccaac aaacgagtaa accgcccctt
    75961 tttcattacc gccgccagtt gcgcggcaaa aggctgccgt tcgtcaatag ctttaacatt
    76021 ggcgctaact gctgctatca gcgctagtgc ggtgatacgc cactcgggtt catcctgcca
    76081 ctttatttgt ctgttcttaa gaaacaggcg aaatccatcc gtcagacaaa catcattaac
    76141 cgtcgtacta cgccgcagac tggcacgctc gccgcgttta tcctgcaact cttcatgcca
    76201 tttacgcagc gcaactttat gctcatcttt tacaatactc attcagcagc ctcctgcttt
    76261 ttctccctgg cggcgttagc attttgcttc tccgctgatg ttgtaaaata tttcttgcgt
    76321 gcggtcatga cgcgttccaa atcaacgggc tcatatggat tggtaaagac acgatcgtca
    76381 aaatcctgac gcgcgaataa ccacatttcc ttttgccatt tactgagtaa ttcgtccgga
    76441 tcctgacctt cttcaatttt gcgcactaat ctcaggaatc gatgctgagt tttgttccag
    76501 aagtcaatat ccacaaaacc gaaatcaagc tttgtggttt tcttctcaga gaaccatgct
    76561 tctttcaacg ctcggtgtaa caagcttaga atgtgtgatg ccgcttgcgc tgccagacgc
    76621 aacttaggta ttag
//
LOCUS       DAZPEB010000004        69859 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZPEB010000004 DAZPEB010000000
VERSION     DAZPEB010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 69859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69859
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.004"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..791
                     /locus_tag="ACHURJ_000231"
     CDS             <1..791
                     /locus_tag="ACHURJ_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1279322.1"
                     /translation="DSLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQT
                     GVCQGDTSRYGPVNIGANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGD
                     FHDLVKLSIQGNLTAPQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITY
                     DCGDTSKIKNSLQMRIDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQ
                     NGILPVDPSGHGTVNMRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(834..1694)
                     /gene="rsmI"
                     /locus_tag="ACHURJ_000232"
     CDS             complement(834..1694)
                     /gene="rsmI"
                     /locus_tag="ACHURJ_000232"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFR1279323.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALVAEIHGVKKNA
                     LYKYALEQQG"
     gene            1758..3785
                     /gene="lpoA"
                     /locus_tag="ACHURJ_000233"
     CDS             1758..3785
                     /gene="lpoA"
                     /locus_tag="ACHURJ_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFR1279324.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKKQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAADVAEQPQPQTADGVASPAQASVSDLTGDQPAAQPVP
                     VSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNVEEL
                     LKSNTPLNVLVLNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIPRSS
                     LGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATTDSG
                     MTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGAMLYAS
                     SRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMGVDA
                     WSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            3743..4138
                     /locus_tag="ACHURJ_000234"
     CDS             3743..4138
                     /locus_tag="ACHURJ_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFR1279325.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEVQARRWLEGKGLRFVAANVN
                     ERGGEIDLIMREGWTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            4158..4748
                     /gene="diaA"
                     /locus_tag="ACHURJ_000235"
     CDS             4158..4748
                     /gene="diaA"
                     /locus_tag="ACHURJ_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFR1279326.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            4758..5333
                     /gene="dolP"
                     /locus_tag="ACHURJ_000236"
     CDS             4758..5333
                     /gene="dolP"
                     /locus_tag="ACHURJ_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFR1279327.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(5447..6487)
                     /locus_tag="ACHURJ_000237"
     CDS             complement(5447..6487)
                     /locus_tag="ACHURJ_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR1279328.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQHYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAICLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEARSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(6560..7195)
                     /locus_tag="ACHURJ_000238"
     CDS             complement(6560..7195)
                     /locus_tag="ACHURJ_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFR1279329.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            7323..7841
                     /gene="yhbO"
                     /locus_tag="ACHURJ_000239"
     CDS             7323..7841
                     /gene="yhbO"
                     /locus_tag="ACHURJ_000239"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFR1279330.1"
                     /translation="MSKKIAVLISDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(7821..8264)
                     /locus_tag="ACHURJ_000240"
     CDS             complement(7821..8264)
                     /locus_tag="ACHURJ_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFR1279331.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     HRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            8315..8617
                     /gene="yhbQ"
                     /locus_tag="ACHURJ_000241"
     CDS             8315..8617
                     /gene="yhbQ"
                     /locus_tag="ACHURJ_000241"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFR1279332.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(8604..9107)
                     /locus_tag="ACHURJ_000242"
     CDS             complement(8604..9107)
                     /locus_tag="ACHURJ_000242"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1279333.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(9101..9625)
                     /locus_tag="ACHURJ_000243"
     CDS             complement(9101..9625)
                     /locus_tag="ACHURJ_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFR1279334.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADVSDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLHVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            9834..10814
                     /locus_tag="ACHURJ_000244"
     CDS             9834..10814
                     /locus_tag="ACHURJ_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFR1279335.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPNIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            10823..11701
                     /locus_tag="ACHURJ_000245"
     CDS             10823..11701
                     /locus_tag="ACHURJ_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFR1279336.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELTSMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLLLTANS
                     DCNGYWRRLAGLELQA"
     gene            11907..12914
                     /locus_tag="ACHURJ_000246"
     CDS             11907..12914
                     /locus_tag="ACHURJ_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HFR1279337.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(13032..14276)
                     /gene="mtr"
                     /locus_tag="ACHURJ_000247"
     CDS             complement(13032..14276)
                     /gene="mtr"
                     /locus_tag="ACHURJ_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFR1279338.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAMGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(14430..16319)
                     /gene="deaD"
                     /locus_tag="ACHURJ_000248"
     CDS             complement(14430..16319)
                     /gene="deaD"
                     /locus_tag="ACHURJ_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFR1279339.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(16312..16392)
                     /gene="yrbN"
                     /locus_tag="ACHURJ_000249"
     CDS             complement(16312..16392)
                     /gene="yrbN"
                     /locus_tag="ACHURJ_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFR1279340.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(16499..17383)
                     /gene="nlpI"
                     /locus_tag="ACHURJ_000250"
     CDS             complement(16499..17383)
                     /gene="nlpI"
                     /locus_tag="ACHURJ_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFR1279341.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDSDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(17492..19627)
                     /gene="pnp"
                     /locus_tag="ACHURJ_000251"
     CDS             complement(17492..19627)
                     /gene="pnp"
                     /locus_tag="ACHURJ_000251"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFR1279342.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRSVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(19875..20144)
                     /gene="rpsO"
                     /locus_tag="ACHURJ_000252"
     CDS             complement(19875..20144)
                     /gene="rpsO"
                     /locus_tag="ACHURJ_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFR1279343.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(20293..21237)
                     /gene="truB"
                     /locus_tag="ACHURJ_000253"
     CDS             complement(20293..21237)
                     /gene="truB"
                     /locus_tag="ACHURJ_000253"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFR1279344.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICFGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITIYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(21237..21638)
                     /gene="rbfA"
                     /locus_tag="ACHURJ_000254"
     CDS             complement(21237..21638)
                     /gene="rbfA"
                     /locus_tag="ACHURJ_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFR1279345.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(21802..24450)
                     /gene="infB"
                     /locus_tag="ACHURJ_000255"
     CDS             complement(21802..24450)
                     /gene="infB"
                     /locus_tag="ACHURJ_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFR1279346.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(24475..25962)
                     /gene="nusA"
                     /locus_tag="ACHURJ_000256"
     CDS             complement(24475..25962)
                     /gene="nusA"
                     /locus_tag="ACHURJ_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFR1279347.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLELGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFSTVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGIDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGVTDEKAGALIMAARNICWFGDEA"
     gene            complement(25990..26442)
                     /gene="rimP"
                     /locus_tag="ACHURJ_000257"
     CDS             complement(25990..26442)
                     /gene="rimP"
                     /locus_tag="ACHURJ_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFR1279348.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGHTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(26649..26725)
                     /locus_tag="ACHURJ_000258"
     tRNA            complement(26649..26725)
                     /locus_tag="ACHURJ_000258"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26689..26691),aa:Met,seq:cat)
     gene            27073..28416
                     /gene="argG"
                     /locus_tag="ACHURJ_000259"
     CDS             27073..28416
                     /gene="argG"
                     /locus_tag="ACHURJ_000259"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFR1279349.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSNDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENMENKGQ"
     gene            complement(28424..30049)
                     /locus_tag="ACHURJ_000260"
     CDS             complement(28424..30049)
                     /locus_tag="ACHURJ_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFR1279350.1"
                     /translation="MTVFNKFARSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFWAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKTFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISSAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDS
                     RVVDANHDVFDYVMFRKEFTEDKQGNPPPEGQG"
     gene            complement(30508..30594)
                     /locus_tag="ACHURJ_000261"
     tRNA            complement(30508..30594)
                     /locus_tag="ACHURJ_000261"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30558..30560),aa:Leu,seq:gag)
     gene            complement(30609..30941)
                     /gene="secG"
                     /locus_tag="ACHURJ_000262"
     CDS             complement(30609..30941)
                     /gene="secG"
                     /locus_tag="ACHURJ_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFR1279351.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWDNLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(31169..32506)
                     /gene="glmM"
                     /locus_tag="ACHURJ_000263"
     CDS             complement(31169..32506)
                     /gene="glmM"
                     /locus_tag="ACHURJ_000263"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFR1279352.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIISKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(32499..33347)
                     /gene="folP"
                     /locus_tag="ACHURJ_000264"
     CDS             complement(32499..33347)
                     /gene="folP"
                     /locus_tag="ACHURJ_000264"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFR1279353.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWLSVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(33437..35371)
                     /gene="ftsH"
                     /locus_tag="ACHURJ_000265"
     CDS             complement(33437..35371)
                     /gene="ftsH"
                     /locus_tag="ACHURJ_000265"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFR1279354.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMIEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(35471..36100)
                     /gene="rlmE"
                     /locus_tag="ACHURJ_000266"
     CDS             complement(35471..36100)
                     /gene="rlmE"
                     /locus_tag="ACHURJ_000266"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFR1279355.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            36226..36519
                     /gene="yhbY"
                     /locus_tag="ACHURJ_000267"
     CDS             36226..36519
                     /gene="yhbY"
                     /locus_tag="ACHURJ_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFR1279356.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(36676..37152)
                     /gene="greA"
                     /locus_tag="ACHURJ_000268"
     CDS             complement(36676..37152)
                     /gene="greA"
                     /locus_tag="ACHURJ_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFR1279357.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            37400..38833
                     /gene="dacB"
                     /locus_tag="ACHURJ_000269"
     CDS             37400..38833
                     /gene="dacB"
                     /locus_tag="ACHURJ_000269"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFR1279358.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMIATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVIPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(38873..40045)
                     /gene="cgtA"
                     /locus_tag="ACHURJ_000270"
     CDS             complement(38873..40045)
                     /gene="cgtA"
                     /locus_tag="ACHURJ_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFR1279359.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(40061..41026)
                     /locus_tag="ACHURJ_000271"
     CDS             complement(40061..41026)
                     /locus_tag="ACHURJ_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR1279360.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(41153..41410)
                     /gene="rpmA"
                     /locus_tag="ACHURJ_000272"
     CDS             complement(41153..41410)
                     /gene="rpmA"
                     /locus_tag="ACHURJ_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFR1279361.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(41431..41742)
                     /gene="rplU"
                     /locus_tag="ACHURJ_000273"
     CDS             complement(41431..41742)
                     /gene="rplU"
                     /locus_tag="ACHURJ_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFR1279362.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            42001..42972
                     /gene="ispB"
                     /locus_tag="ACHURJ_000274"
     CDS             42001..42972
                     /gene="ispB"
                     /locus_tag="ACHURJ_000274"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFR1279363.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            43200..43478
                     /gene="sfsB"
                     /locus_tag="ACHURJ_000275"
     CDS             43200..43478
                     /gene="sfsB"
                     /locus_tag="ACHURJ_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFR1279364.1"
                     /translation="MESNFIDWHPPDIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(43526..44785)
                     /gene="murA"
                     /locus_tag="ACHURJ_000276"
     CDS             complement(43526..44785)
                     /gene="murA"
                     /locus_tag="ACHURJ_000276"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFR1279365.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     EHLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIKRVKGE"
     gene            complement(44840..45094)
                     /gene="ibaG"
                     /locus_tag="ACHURJ_000277"
     CDS             complement(44840..45094)
                     /gene="ibaG"
                     /locus_tag="ACHURJ_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFR1279366.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(45254..45547)
                     /gene="mlaB"
                     /locus_tag="ACHURJ_000278"
     CDS             complement(45254..45547)
                     /gene="mlaB"
                     /locus_tag="ACHURJ_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFR1279367.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLQLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(45547..46182)
                     /gene="mlaC"
                     /locus_tag="ACHURJ_000279"
     CDS             complement(45547..46182)
                     /gene="mlaC"
                     /locus_tag="ACHURJ_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFR1279368.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(46201..46752)
                     /gene="mlaD"
                     /locus_tag="ACHURJ_000280"
     CDS             complement(46201..46752)
                     /gene="mlaD"
                     /locus_tag="ACHURJ_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFR1279369.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(46757..47539)
                     /gene="mlaE"
                     /locus_tag="ACHURJ_000281"
     CDS             complement(46757..47539)
                     /gene="mlaE"
                     /locus_tag="ACHURJ_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFR1279370.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(47547..48356)
                     /gene="mlaF"
                     /locus_tag="ACHURJ_000282"
     CDS             complement(47547..48356)
                     /gene="mlaF"
                     /locus_tag="ACHURJ_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFR1279371.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            48566..49543
                     /locus_tag="ACHURJ_000283"
     CDS             48566..49543
                     /locus_tag="ACHURJ_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFR1279372.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            49557..50543
                     /gene="kdsD"
                     /locus_tag="ACHURJ_000284"
     CDS             49557..50543
                     /gene="kdsD"
                     /locus_tag="ACHURJ_000284"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFR1279373.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            50564..51130
                     /gene="kdsC"
                     /locus_tag="ACHURJ_000285"
     CDS             50564..51130
                     /gene="kdsC"
                     /locus_tag="ACHURJ_000285"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFR1279374.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSELLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            51127..51702
                     /gene="lptC"
                     /locus_tag="ACHURJ_000286"
     CDS             51127..51702
                     /gene="lptC"
                     /locus_tag="ACHURJ_000286"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFR1279375.1"
                     /translation="MSKARRWVTIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            51671..52228
                     /gene="lptA"
                     /locus_tag="ACHURJ_000287"
     CDS             51671..52228
                     /gene="lptA"
                     /locus_tag="ACHURJ_000287"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFR1279376.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            52235..52960
                     /gene="lptB"
                     /locus_tag="ACHURJ_000288"
     CDS             52235..52960
                     /gene="lptB"
                     /locus_tag="ACHURJ_000288"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFR1279377.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            53008..54441
                     /gene="rpoN"
                     /locus_tag="ACHURJ_000289"
     CDS             53008..54441
                     /gene="rpoN"
                     /locus_tag="ACHURJ_000289"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFR1279378.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTSETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            54464..54751
                     /gene="hpf"
                     /locus_tag="ACHURJ_000290"
     CDS             54464..54751
                     /gene="hpf"
                     /locus_tag="ACHURJ_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFR1279379.1"
                     /translation="MQLNITGNNVEITEALREFVRAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            54869..55360
                     /gene="ptsN"
                     /locus_tag="ACHURJ_000291"
     CDS             54869..55360
                     /gene="ptsN"
                     /locus_tag="ACHURJ_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFR1279380.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            55406..56260
                     /gene="rapZ"
                     /locus_tag="ACHURJ_000292"
     CDS             55406..56260
                     /gene="rapZ"
                     /locus_tag="ACHURJ_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFR1279381.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            56257..56529
                     /gene="npr"
                     /locus_tag="ACHURJ_000293"
     CDS             56257..56529
                     /gene="npr"
                     /locus_tag="ACHURJ_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFR1279382.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            56743..57375
                     /gene="yrbL"
                     /locus_tag="ACHURJ_000294"
     CDS             56743..57375
                     /gene="yrbL"
                     /locus_tag="ACHURJ_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFR1279383.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAYLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHHISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(57372..58100)
                     /gene="mtgA"
                     /locus_tag="ACHURJ_000295"
     CDS             complement(57372..58100)
                     /gene="mtgA"
                     /locus_tag="ACHURJ_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFR1279384.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHRGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(58097..58750)
                     /gene="elbB"
                     /locus_tag="ACHURJ_000296"
     CDS             complement(58097..58750)
                     /gene="elbB"
                     /locus_tag="ACHURJ_000296"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFR1279385.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(58980..61316)
                     /gene="arcB"
                     /locus_tag="ACHURJ_000297"
     CDS             complement(58980..61316)
                     /gene="arcB"
                     /locus_tag="ACHURJ_000297"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFR1279386.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(61412..62341)
                     /locus_tag="ACHURJ_000298"
     CDS             complement(61412..62341)
                     /locus_tag="ACHURJ_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFR1279387.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            63016..67476
                     /gene="gltB"
                     /locus_tag="ACHURJ_000299"
     CDS             63016..67476
                     /gene="gltB"
                     /locus_tag="ACHURJ_000299"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFR1279388.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLNVDELAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            67489..68907
                     /gene="gltD"
                     /locus_tag="ACHURJ_000300"
     CDS             67489..68907
                     /gene="gltD"
                     /locus_tag="ACHURJ_000300"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFR1279389.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEQFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENKDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            69091..69495
                     /locus_tag="ACHURJ_000301"
                     /pseudo
     CDS             69091..69495
                     /locus_tag="ACHURJ_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417687.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
     gene            69587..>69859
                     /locus_tag="ACHURJ_000302"
     CDS             69587..>69859
                     /locus_tag="ACHURJ_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR1279390.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIK"
BASE COUNT        17102 a        18942 c        17347 g        16468 t
ORIGIN      
        1 atgacagcct ggatattaaa accacattac aggcaaacga cattccagga ctcacaaccg
       61 accaggttgt ctctgttaac acccgattca cacagataaa aagcagcacc gtatattctg
      121 ctgcaaccca aacgggtgtt tgccagggtg acacgtctcg ttatggaccc gttaatattg
      181 gtgcaaatac cacctttacc ctgtatgtca ccaagccatt tctcggctcg atgaccattc
      241 cgaaaacgga tattgccgtc attaaaggcg cgtgggtcga tggaatggga agcccgtcta
      301 caggtgactt ccatgattta gtcaagttat cgattcaggg aaatctcacc gccccacagt
      361 cgtgcaaaat taatcagggc gatgttatta aggttaattt tggattcatc aatggtcaga
      421 agtttaccac ccgcaatgcc atgccagacg gttttactcc agtagacttt gatatcactt
      481 atgactgtgg tgatacttca aagattaaaa actcgttgca aatgcgcatc gacggtacaa
      541 ctggggtagt agaccagtac aacctggtcg ccaggcgaag aagttcagac aatgtgcccg
      601 atgtcggtat tcgtattgaa aatctcggcg gcggagttgc aaatattcct tttcagaacg
      661 gtatccttcc cgttgatcct tccgggcatg gcaccgtcaa tatgcgcgcc tggccagtta
      721 atctggtcgg tggtgagctg gaaacaggaa aatttcaggg cacagccacc attaccgtca
      781 tggtgcggta aaagaagacg ttgcgttttg attaacgcaa cgcttcggca ctgttacccc
      841 tgctgctcca gcgcatactt atacagcgca tttttcttca cgccatgaat ttctgcgacc
      901 agcgccgccg cttttttcag cggcagttct gcctgtagca gcgccagcgt gcgcagggca
      961 tcggcgggta agtcttcttc ctgtgcttta tgaccttcga caatcagcac catttcgcct
     1021 ttgcgacggt tttcatcttc ctttacccac gccagcagtt cgccaacggg cgcgccgtga
     1081 atggtttccc aggttttggt cagctcacgc gccagaacca cgtagcggga ttcgcctaat
     1141 accgcaacga tatcttccag gctatctaac agacggtggg tggattcata aaaaatcagc
     1201 gtgcgcggct ccgcttcaat ggcttttagc gcatcacggc ggccttttga tttggcaggt
     1261 aaaaagcctt cgtaacagaa acggtcagag ggtaaacccg cggcgcttaa cgcagtgata
     1321 gcagcacacg gcccgggtag cggcaccacg cgaatccccg cttcacggca ggtacgcacc
     1381 agatggtagc caggatcgtt aattagcggc gttccggcat cggaaaccag cgcaatgttt
     1441 tgcccctctt gcagcttcgc cagcagcgtt tcggcttttt gttgttcgtt atggtcgtgc
     1501 agcgcaaaca accgggcatt aatcccaaaa tgttgcagca ataaaccggt gtgacgagta
     1561 tcctcggcgg caatcagatc aacggcctgt aatacctcta acgcacgctg ggtgatatcc
     1621 gccagattgc cgattggcgt cggtacaatg taaagctggc cctgagaatt atccgccgat
     1681 tggtgttgtt tcattgtgtc gtccgtattg ccgatttaat attgagcatt gcgtaaaaaa
     1741 atatcactgg atacattatg gtaccctcaa cattttctcg tttgaaagcc gcgcgttgtc
     1801 tgcctgttgt tctggcagcc ctgattttcg ccggttgtgg cacccatact cccgatcagt
     1861 ccactgctta tatgcagggc acggcgcagg ctgattctgc cttttatctt cagcagatgc
     1921 agcaaagctc tgatgatacc aggatcaact ggcaattact cgccattcgt gcactggtga
     1981 aagaaggtaa aaccgggcag gcggttgagt tgtttaacca actaccgcaa gaactgaacg
     2041 attctcagcg tcgcgagaaa acactgctgg cggtagagat taaactggcg cagaaagatt
     2101 ttgctggcgc gcaaaacttg ctggcgaaaa tcacacctgc cgatttagaa caaaaccagc
     2161 aagcgcgtta ctggcaggca aaaatcgatg ccagccaggg gcgtccttcc attgatttac
     2221 tgcgcgcgtt aattgctcag gaaccgctgc tcggcgcgaa agaaaaaaag cagaatattg
     2281 atgccacctg gcaggcgctc tcctccatga ctcaggaaca ggcgaatacg ctggtgatca
     2341 acgccgacga aaatattctg caaggctggc tggatctgca gcgcgtctgg tttgataacc
     2401 gtaacgatcc cgatatgatg aaagccggga tcgccgactg gcagaaacgt tatccgaaca
     2461 atccgggcgc gaaaatgctg ccaacgcagt tggttaacgt aaaagcgttt aaaccagcct
     2521 cgaccaacaa aatcgccctg ctgttgccgc tgaatggcca ggcagcggta tttggtcgca
     2581 ctattcagca aggctttgaa gcggcgaaaa atatcggcac tcagccagtg gcggctcagg
     2641 tagctgccgc agacgtagca gaacaacctc agccgcaaac tgcggatggc gttgccagcc
     2701 cggcacaagc ctcggttagc gatctgaccg gtgatcagcc tgcggcccag ccggtgcctg
     2761 taagcgcccc ggcgacaagc accgcagcgg taagcgcacc cgcaaatcca tccgcagagc
     2821 tgaaaatcta cgatacctca tcacaaccac ttagccagat cttaagccag gttcagcagg
     2881 atggcgcgag tattgtggtt ggtccgttgc tgaaaaataa cgttgaagag ttgcttaaga
     2941 gcaacacccc gctgaacgtg ctggtactga accagccgga gaatatcgaa aaccgcgtca
     3001 atatttgtta cttcgcgctt tcaccggaag acgaagcgcg cgatgcagcg cgtcatattc
     3061 gtgaccaggg taaacaagcg ccgctggtgc tgatcccacg cagttcattg ggcgatcgcg
     3121 tagccaatgc gtttgcgcag gagtggcaga aactgggcgg cggcaccgtt ctgcaacaaa
     3181 aatttggttc caccagcgaa ttacgcgcgg gtgttaacgg cggttctggt atcgctttaa
     3241 cgggtacccc gattactccc agagcgacaa ccgactccgg catgacgacc aacaatccaa
     3301 cgctgcaaac cacgccaacc gatgaccagt tcaccaataa tggcggtcgt gtcgatgcgg
     3361 tgtacattgt ggcaacgccg ggtgaaatcg cttttatcaa accgatgatc gccatgcgta
     3421 acggtagcca gagcggtgca atgctgtacg ccagctcccg cagcgcacaa ggcaccgcag
     3481 gcccggattt ccgtctggag atggaaggtt tgcagtacag cgaaatcccg atgctggcgg
     3541 gcggtaatct gccgttaatg cagcaggcac tcagcgcggt gaataacgat tattcactgg
     3601 ctcgcatgta tgcgatgggc gtcgatgcct ggtcgctggc aaatcatttc tcacaaatgc
     3661 gccaggttca gggttttgaa atcaacggta ataccggaag cctgacggca aacccggatt
     3721 gcgtgattaa caggaagtta tcatggctac agtaccaaca aggtcaggta gtccccgcca
     3781 gttaaccacc aaacagaccg gcgatgcgtg ggaagtacaa gcgcgtcgct ggctggaagg
     3841 caaaggactg cgatttgtcg ccgctaacgt gaacgagcgt ggcggtgaga tcgatctgat
     3901 aatgcgtgaa ggctggacca ccgtttttgt cgaggtgcgt taccgccgct ctgcgcttta
     3961 tggcggcgcg gcagccagtg tgacccgcag caaacaacac aaattattac agactgcccg
     4021 cttgtggctc gcgcgtcata atgggagttt tgatactgtg gattgccggt tcgatgtggt
     4081 agccttcacc gggaatgagg ttgagtggat taaggatgcc tttaatgacc actcataatt
     4141 aaggtttaag gattagcgtg caagaaagaa ttaaagcttg cttcactgaa agcattcaaa
     4201 ctcaaattgc ggcggcagag gcgcttccgg atgccatctc ccgtgcagcc atgacgctgg
     4261 ttcagtctct gctcaatggc aacaaaatcc tctgttgtgg taatggaact tccgctgcca
     4321 atgcacagca ttttgctgcc agcatgatca accgtttcga aacggagcgg cccagcttac
     4381 ctgccattgc actaaatact gataatgttg tcttaacggc gattgccaac gatcgcttac
     4441 atgatgaagt gtatgcaaaa caggtgcggg cgctgggtca tgcgggagat gtattgttag
     4501 ccatttccac ccgtggcaac agccgcgata ttgttaaagc agttgaagcc gccgttacgc
     4561 gtgatatgac cattgtggca ttgaccggct atgacggcgg cgaacttgca ggtttgttag
     4621 ggccacagga tgtggagatc cgcattcctt cgcatcgtag tgctcgcatt caggaaatgc
     4681 atatgctgac ggtaaattgc ctgtgcgatc tgatcgataa cacgcttttc cctcaccagg
     4741 atgattaagg agaatacatg aaggcattat cgccaatcgc agtccttatt tccgcgctgc
     4801 tgttgcaagg ttgtgttgcc gctgccgtag tgggtaccgc tgctgtgggt accaaagccg
     4861 caactgaccc acgcagtgtc ggtacccagg tggacgatgg taccctggaa gtgcgcgtga
     4921 acagcgcatt gtcgaaagac gaacagatta agaaagaaac ccgtattaat gtcacggcgt
     4981 atcagggcaa agtgctgctg gttgggcagt caccaaatgc tgaactttcg gctcgcgcca
     5041 aacagattgc tatgggcgta gacggtgcca acgaagtgta taacgagatt cgtcagggcc
     5101 agccgattgg tctgggcgaa gcatctaacg atacgtggat caccaccaaa gtgcgttcgc
     5161 agctcttaac cagcgacctg gtgaaatcgt ccaacgtgaa agtgaccacc gaaaacggtg
     5221 aagtgttcct gatggggctg gtgactgaac gtgaagcgaa agcggcggca gatattgcca
     5281 gccgggtgag cggcgtaaag cgggtaacca cagcgtttac gtttattaaa tagcagcccc
     5341 ttgtaatgcc tgatgcgacg cttgccgcgt cttatcaggc ctacaacgat gcaaaccgta
     5401 ggtcggataa ggcgtttacg ccgcatccga catgttttcc ctcaaatcag aacaacagcg
     5461 ccagcccgcc gacaatcaca ccagacactg ccaccatcgc ccctgtcagc cataaggctt
     5521 tcgccgggaa cgctttgcgc agcataatca gtgacggcaa actcactgcc gggagcgtca
     5581 tcaacaatgc cagcgccgga gcggttccca tacctgccag catcatcgtt tgtacaatcg
     5641 gaatttctgc tgccgtggga atgacaaaca agcatcctgc taccgccatc gccaccaccc
     5701 acatcaggct gttatcgaca gcaccatcgg catgggggaa taaccagacg cgtgcggcac
     5761 ccaacaccag tactgcaagg atgtaaaccg ggatcgtact ccagaaaagt gtccatagcg
     5821 ccctgcccca gcggctaaaa aaaccgctcc gtgcttccgg tatgtcaatt tcgaccggtg
     5881 cctgcgtttg cggtgtttca cgcacccatt tttgcaccag cgtcgcaatc agcaacacca
     5941 tcaccagccc ggccaccaga caaatcgccg caaaacccca gccgaggaca aaccccataa
     6001 acaccagcgt cgccgggttt aacaccggat tgcccatcca gaatgccagc gcaccgccca
     6061 tcgatacctg ttggcgacgc atccccgccg cgaccggagc cgcacagcag gtacacatca
     6121 tgcccggcag cgaaaacagc gttcccagca gcgtgccgcg aaagcgcgat tgcccaagcg
     6181 tacgcaacaa ccagtcacgc gggatcagca cctgaatcaa cgaaccgagg atcaccccca
     6241 gcaccgccgc tttccatact gcgaggaaat agatcatcgc gtaatccaac gccgcttgcc
     6301 acggattagc atccgcctgc gcaaggatag atttaccgat actgtgggtt tcggctgcag
     6361 taaacgcttt gccgtagtaa tgttgccatt tcacatacca gagaccggca atgacaacga
     6421 gaaagaaaag cgcgggtttc caccactgaa tgggtgttgc cgcctgagat gaagactgac
     6481 cagtcatagc attccctagg aataagttat gataattgag cgcgtgaata ttacgctcac
     6541 tatcaattct tgggaataat tattcagctc ttttgcgtaa ttctgaagag cttaaaatcg
     6601 tcacgccttc atgctcaggc cgcatcgcct ctgccagcat gacccgcgcc acgtctctcg
     6661 catcaatgga tttccagtta cctggtaaca aacggaacag cggcgcaaaa agcgtttcgt
     6721 tcatccgctg tttgctacga tcgcccagta acatcgacgg gcgagcaatg gtcaatttcg
     6781 gccagttctg ggcaattaat gcttcttcca tctccccttt gacgcggtta tagaaaaacg
     6841 gcgagtgggc attggccccc atagcactga ccaccagcat atgctgcgcg cccagccgcc
     6901 gcccggttaa tgcggtatcc actaccagcg tgtaatcggc atgaataaac gcctctttgc
     6961 tccccgcttc tcgccgcgtg gtgccgagac aacaaaacac aatgtcgatg ggttcggtga
     7021 cctgcgccaa cgcgtcactc agttgcggat catggggatt aaacaccccg ggcatatcgc
     7081 ccaacggacg tcgcgtcggc gcagcaatgg cgttaacttt cggttcatta atcaacatcc
     7141 gcagcaggtg accgcccacc agccccgttg cgcctgtaat cagtacctga ctcatcttcg
     7201 ctcctttaca gaattgtctg ccttgcgctc cacggctcat gcaccagtct taatagacca
     7261 tgaggtaatt atccccgatt gtgggaaatt cgcctcatcc aatgcaacaa cgcggaggaa
     7321 gcatgagtaa gaaaattgcc gttttaatca gtgatgaatt tgaggattca gaatttactt
     7381 cacccgcaga cgagttccgt aaagccggac acgaagtaat taccattgaa aagcaagcgg
     7441 gtaaaacggt gaaaggcaaa aaaggagaag ccagcgtgac catcgataaa tccatcgatg
     7501 aagtgacgcc agcggagttt gatgccctgc tgctaccggg cggccattca ccggattatc
     7561 tgcgtgggga caaccgtttt gtcaccttta cccgtgattt tgtgaatagc ggcaaacctg
     7621 ttttcgccat ctgtcacggt ccgcagctgc tgatcagcgc cgatgtgatt cgggggcgca
     7681 aactgaccgc agttaaaccg atcattattg atgttaaaaa tgcgggcgcg gaattttacg
     7741 atcaggaagt cgtggttgat aaagatcagc tggttaccag ccggacaccg gacgatctgc
     7801 cagcgtttaa ccgcgaggcg ttacgcctgc tcggtgcctg agtcgcgtag ccaaatcatt
     7861 tttttaccaa agcccagcgt gttgtcggtg aatttgattt catcgagccg gatttcccac
     7921 accggggccg acagcattct ggcaaccgga aagcgatgat tgtacgcctt gcgcgcgagg
     7981 tcgctttcct caccttccag cctgcggatc tcacctttaa actgcacacc gcgaattaac
     8041 gctaccgttt tcggctgacc gtttaccgtt ccggcaaccg cagcctgcgg cccactcatc
     8101 tgcgcgtggc gcgttttttc ttccgtcaaa atgtagaagg cgactttctg cgcatcaaac
     8161 agataaaaag cattagcgca ccacagttcc ccttcctgct gcacacacca ggtgacaaca
     8221 tgttgttttg ccaaccaacg gctgatggtg atgagtgttt ccattgctgt tctcttttat
     8281 actgtgggcc tgaccttaac atgcgacgca gacgatgaca ccctggtttc tttacttgat
     8341 ccgtaccgcc gacaataagc tttataccgg gatcaccacg gatgtcgaac gccgctatca
     8401 gcagcaccaa agcggcaaag gggcgaaagc actgcgcggg aaaggagaac tgacgctggc
     8461 gttttctgcg ccagtgggcg accgttcgct ggcactacgg gcggaatatc gcgttaagca
     8521 actgaccaaa cggcaaaaag agcgtctggt agcggaaggc gcagggtttg cagaactgtt
     8581 gagcagcctg caaaccccgg agattaaaag cgattgaaat gctcgtgata ctcaaccaga
     8641 ccggttacgc cattcagcgc gtcatccgct aaacgatgta cctggaaggc gctttcagtg
     8701 cccggccagc ggcaacgcag atcgtgatgc gccgccagtt caaagccgaa acgactgtac
     8761 agcgccggat cgcccagcgt caccactgcg gcatagccga actcattaag cgaatcgagt
     8821 ccttcataga ccagttggcg tgccagcccc tgtccacggt atttttcatc aaccgccaac
     8881 ggtgccatgc cgacccattg caggtcttcg ccctgcacat caaccggact aaatgccaca
     8941 tagccaatga cctgaccttc gtcatctgtc gccaccagcc ccagcgtcag aaagccatct
     9001 tcacgcagat cgtgaaccag cttcgcttcc gcatcacttt cgaatgagcg acgcagcagg
     9061 gcatcaatac ccggcgcatc aatgggaatt tctactcgaa ttagcatggt tcacctaccg
     9121 atgtctgttt ggtttcaggc gcggttttca ttcccgcctc aacaaaatcc gccagttgca
     9181 gcagcatcat gcgtaaggct ttcggcattt gctccagttc aatggcgtcc atcaggtttt
     9241 tcacatgcag ccccagctcc gtatcgcctt caatcaccag ccgacgctgg aagaagagcg
     9301 tatctggatc ttgtttacgc gccgcaatca tcagcagatc gctgacgtcg gcactaaaac
     9361 tcacatcagc ttgcgcgttc tggctgacca ccagtttgcc attcaccacc gaggtaaacc
     9421 attgcaggtc aatatcacgc acatgaatac ttaaccagcg gccttcaaga aactccagct
     9481 cgccatcatc cagcgcctga cggaattgcc agcttaagac ctgctcaaga acctggcgtt
     9541 ttagcgcaaa tggcgtcagt tttaccggta cactcaacag agatggcccc aaatgcacaa
     9601 tacgggaacg aagtttatcc aacacgagtt ttactccctg tttcaacaat catcctattt
     9661 tgccatatca gaaaaataac atagcggtat aaatcaacaa ttccatatga aattgctgct
     9721 accaccaata caactttaac tgccttaaat caaaaattgt cgcagcaagg ttaactaaaa
     9781 tcccagttcg ttaacatttt tgcgttttga tagcgcaacc ttcaggaaaa attatggagc
     9841 tgctctgccc tgccggaaat ctcccggcgc ttaaggcggc catcgaaaac ggcgcagatg
     9901 ctgtttatat cgggctaaaa gatgatacca atgcccgtca cttcgccggc cttaacttta
     9961 ccgagaaaaa attgcaggaa gcggtgagtt ttgtccatca acatcgccgc aaacttcaca
    10021 tcgcgattaa cacttttgcg catccggacg gttacgcccg ttggcagcgc gccgtggata
    10081 tggcggcgca gctgggtgcc gacgcgctga tcctcgccga cctcgccatg ctggaatacg
    10141 ccgccgagcg ttatccgaat attgagcgcc acgtatcggt gcaggcttcg gcgaccaatg
    10201 aagaggcgat taacttttat catcgccatt ttgacgttgc ccgcgtggtg ctgccgcgcg
    10261 tgttgtcgat tcatcaggtg aaacagctgg cacgggtcac acctgtacca ctggaagtgt
    10321 ttgctttcgg cagcctgtgc attatgtcgg aaggccgttg ctatctgtcg tcgtatctga
    10381 cgggtgagtc gcctaacacc gtgggcgcgt gttctccggc ccgtttcgtg cgctggcaac
    10441 aaacgccgca ggggctggaa tcccgcctga acgaagtgct gatcgaccgt tatcaggacg
    10501 gcgaaaacgc aggttatccg acgctgtgta aagggcgtta tctggtggac ggcgagcgct
    10561 atcacgcgct ggaagaacca accagtctca ataccctgga actgctgccg gagttaatgg
    10621 cggcgaatat tgcttcggtg aaaattgaag gccgccagcg tagcccggcg tatgtcagcc
    10681 aggtggcgaa agtctggcgt caggctatcg accgttgtaa ggccgatccg caaagcgcgt
    10741 ggatggagac gctcgggtcg atgtccgaag gcacgcagac cacccttggc gcgtatcacc
    10801 gtaaatggca gtgagaagag caatgaaata ttccttaggg ccagtgctgt ggtactggcc
    10861 aaaagagacg ctggaagatt tttatcagca ggccgccacc agcagcgccg acgtgattta
    10921 tcttggtgaa gcggtatgca gcaagcgccg ggcaaccaaa gttggcgact ggctggagat
    10981 ggcaaaatcg ctcgccggaa gtggcaagca gattgtgctc tccacgctgg cgctggtgca
    11041 ggcatcctct gaactgggcg aactgaaacg ctatgttgag aacggtgagt ttctgattga
    11101 agccagcgat ctcggcgtgg tgaatatgtg cgctgaacgc aaactgccgt tcgtcgccgg
    11161 gcacgcgctg aactgctaca acgcggtgac gctgaaaata ttgctcaaac agggcatgat
    11221 gcgctggtgt atgccggtgg agctttcccg cgactggctg gtgaatctgc ttaatcagtg
    11281 cgatgagctg ggcattcgca accagtttga agtggaagtc ctgagctacg gtcatctacc
    11341 gctggcctac tccgctcgct gctttaccgc gcgttcggaa gaccgcccga aagatgagtg
    11401 tgaaacctgc tgcattaagt atccgaacgg gcgcaacgtg ctgtcgcagg aaaaccaaca
    11461 agtgtttgta ctcaatggca ttcagaccat gagcggctac gtttacaacc tcggtaacga
    11521 gctgacatcc atgcagggat tggttgatgt ggtccgcctg tcaccgcagg gtactgacac
    11581 tttcgcgatg ctcgacgcct tccgcgctaa tgaaaatggc gctgcgccac tattgttgac
    11641 ggcaaacagc gactgtaacg gctactggcg gcggctggca ggactggagc tgcaagccta
    11701 agtaaatatg ctcgtcatac ttcaagttgc atgtgctgca tctgcattcg ttcaccccag
    11761 tcacttactt atgtaagctc ctggggattc actctcttgt cgccttcctg caactcgaat
    11821 tatttagagc acagctcact ttgttaacaa ctttaactac tctttaatgc agtattaaag
    11881 attaatcggt aacaaagtga gctgttatga ctgataaaac cattgcgttt tcgttactcg
    11941 atctggcccc cattcccgaa ggttcttcag cgcgagaagc attctcccac tctctcgatc
    12001 tcgcccgtct ggctgaaaag cgcggctatc atcgctactg gctggcagaa caccacaata
    12061 tgactggcat tgccagtgct gccacgtcgg tattgattgg ctatctggcg gcgaatacca
    12121 ccacgctgca tctggggtct ggcggcgtga tgttgcctaa ccactcaccg ttggtcattg
    12181 ccgaacagtt cggcacgctc aatacactct atccggggcg aatcgatttg gggctgggtc
    12241 gtgcgccagg tagtgaccaa cggacaatga tggcgctgcg tcgccatatg agcggcgata
    12301 ttgataattt cccccgcgat gtcgcggagc tggtggactg gtttgacgcc cgcgatccca
    12361 atccgcatgt gcgcccggta ccaggctatg gcgagaaaat ccccgtgtgg ttgttaggct
    12421 ccagccttta cagcgcgcaa ctggcggcgc agcttggtct gccgttcgcg tttgcctcac
    12481 acttcgcgcc ggatatgttg ttccaggcgc tgcatcttta tcgcagcaac ttcaaaccgt
    12541 cggcacgact ggaaaaacca tacgcgatgg tgtgcatcaa tattatcgcc gccgacagca
    12601 accgcgatgc cgaattcctg tttacctcaa tgcagcaagc ctttgtgaag ctgcgccgcg
    12661 gcgaaaccgg gcaactgccg ccaccgattc aaaatatgga tcagttctgg tcgccgtccg
    12721 aacagtatgg tgtgcagcag gcgctgagta tgtcgttggt aggtgataaa gcgaaagtgc
    12781 gtcatggctt gcagtcgatc ctgcgcgaaa ccgacgccga tgagattatg gtcaacgggc
    12841 agattttcga ccaccaggcg cggctgcatt cgtttgagct ggcgatggat gttaaggaag
    12901 agttgttggg atagtgtgtc ttaacgcggg aagccttatc cgagctggca acgctgtcct
    12961 acgtagacct gataagcgaa gcgcatcagg cattgtgtag gcagcagaaa tgtcggataa
    13021 ggcaccgctg attactgata caccggcagt aaattaaagc tcgataaaat atgtaccaat
    13081 gcgttgccga cgccaaacac cagaatcagc gtaatcatcg gcttgccgcc ccagacgcgg
    13141 aatttcgggc tgccaaagcg tttacgcgat gcgcgggcca gcagtgccgg aacaattgcc
    13201 gcccagatgg tagccgctaa accagcataa ccaatggcgt acaggaatcc attcgggaac
    13261 agcagaccac ccacaacagg cggtgcaaag gtcaacaatg ctgttttcaa gcggcccata
    13321 gccgagtcat cgaaaccaaa cagatctgcc agatagtcaa acaaacccag cgttacgcca
    13381 aggaacgaac tcgctaccgc aaagtttgag aacacgacca gcagaaggtc caggctacgg
    13441 ctgttcagca cgccgcttaa tgcctgtacc agcacatcaa tattaccgcc cttctctgca
    13501 ataccgataa actccggacg cgggatgtta cccatcgtcg ccagcaacca aatggtatac
    13561 agcgccagtg ccatcagcgt accgtacacc aggcatttca cgatggtttt cggatctttg
    13621 ccgtaatact tcatcaggct tggcacgtta ccgtgataac caaacgacgc caggcagaac
    13681 ggcaatgtca tcagcagata cggcgcataa gatgcattgc tttctgcaac gttgaacaat
    13741 gtcgcaggct gcacatgccc caacaggctg ccaaaggtga ggaagaaggt aatgactttc
    13801 gcccccagca caatcgccgt catgcggctg acggccttag tactcaacca caccacaaac
    13861 gctaccagca gtgcaaaacc aaaacccgcc gcccgtgccg ggacgtttag cgacatctct
    13921 gcgaaggtgt gatgcagaat tgaaccactg gcagaaatat aggcataggt caggatatag
    13981 agcacaaagg caatggaaat accgttgacc acgttccagc ctttgcccag caaatctttg
    14041 gtgatggtgt caaaactcga accgattcgg taattcaggt tagcttccag aatcatcaag
    14101 ccggaatgca gcatacagaa ccaggtaaag atcagcgccg ccattgacca gaaaaaccac
    14161 gccccggaca tgaccactgg cagagaaaac atccctgcgc caataatggt gccgccgata
    14221 atcaccacgc cgccaagcag cgacggtgac gtttgggtgg tggttagtgt tgccatgagg
    14281 gcttctctcc agtgaaaaat agtgcgactg cgttgttatg cattgcactg taccagtacg
    14341 cgagtacaaa agacagaaaa aaagccccga tggtaaaaat cggggctgta tatattattt
    14401 tacagattgt gttcgctgtt cagcgatgat tacgcatcac caccgaaacg acgacgaccg
    14461 gtagagtcat cacgacgcgg agcgcggcct tcacgacgtt cgccgctaaa acgacgacca
    14521 tcaccacggc caccttcacg gcgttcaccg ctgaagttac gaccgccttc acgacgttcg
    14581 ccaccgaaac cacgaccacc gccacgacgc tcaccgccag tatgcggctg tgcatcgccc
    14641 agtaactgca tgttcatcgg cttgttgaga atgcgagtgc gcgtaaagtg ttgcagcact
    14701 tcacccggca tacctttcgg cagttcgatg gtggagtgag aagcaaacag cttgatgtta
    14761 ccaatgtaac ggctgctgat gtcgccttcg ttagcaatcg caccaacgat atgacgaact
    14821 tcaacaccat catcacggcc cacttcaatg cggtacagct gcatatcgcc aacatcacga
    14881 cgttcacgac gcggacgatc ttcacggtca ccacgcgggc cacggtcgtt acgatcgcgc
    14941 ggaccacggt catcacggtc acggaattca cgtttcggac gcatcggcgc atctggcggt
    15001 acgatcagag tacgttcacc ctgtgccatt ttcagcagtg ccgcagccag agtttcgaga
    15061 tccagctctt caccttctgc agtcggctga attttgctca gcagtgcgcg gtattgatcc
    15121 agatcgctgc tttccagctg ctgctgtact ttagcggcga atttttccag acggcgtttg
    15181 cccagcagtt ctgcgttcgg cagttctact tccggaatag tcagcttcat agtacgttca
    15241 atgttgcgca gcagacgacg ctcgcggttc tcaacgaaca gcagcgcgcg gccagcacga
    15301 cccgcacgac cggtacgacc gatacggtga acgtaagact cagaatccat cgggatatcg
    15361 tagttaacta ccaggctgat acgctcaacg tccaggccac gggctgcaac gtcggtcgca
    15421 atcaggatgt ccagacgacc atctttcagg cgttccagtg tctgttcacg cagcgcctgg
    15481 ttcatgtcac cgttcagcgc ggcgctgttg tagccgttac gctcaagagc ttcagccact
    15541 tccagagtcg cgtttttggt acgaacgaag ataatcgccg catcaaaatc ttccgcttcc
    15601 aggaaacgta ccagtgcttc gtttttgcgc ataccccaga cagtccagta gctctggctg
    15661 atgtcaggac gggtagtcac gctggactga atgcgcactt cctgcggctc tttcataaag
    15721 cggcgggtaa tgcgacgaat cgcttccggc atggttgcag agaacagagc ggtctgatga
    15781 ccttccggga tctgcgccat aatggtttca acgtcttcga tgaagcccat gcgcagcatt
    15841 tcgtcagctt catccagaac cagaccgctc agtttagaga ggtccagagt gccacgtttc
    15901 aggtggtcca gcagacggcc cggagtaccg acaacgatct gcggcccctg acgcagggcg
    15961 cgtaattgca cgtcataacg ctggccgccg tacagagcaa ccacatttac gccgcgcatg
    16021 tgtttagaga aatccgtcat tgcttcagca acctgtaccg ccagttcgcg ggtcggtgcc
    16081 agcaccagaa tctgtggtgc tttcagctca ggatcaagat tctgcaacag aggtaaagag
    16141 aatgctgcag tttttccgct ccccgtctgg gccataccca gaacgtcgcg gccattcagc
    16201 agatgtggaa tacactctgc ctgaattgga gatggttttt cgtaacccag atcgttaagg
    16261 gcttcaagga taggagcctt caggcccaga tctgcaaaag tggtttcgaa ttcagccatg
    16321 tagtacgtgt gcctcaaaat taatggcggc cagtctacat aactcatcat gaaattgatc
    16381 agcaattttc attgaaaagt gtgaaccggc tcaaagtagg tgtattaacg aacaacaacg
    16441 ccctcacccc ttaaggtgat ggcaatcaaa aaagattacg ggctgatgtg tacgtcagct
    16501 attgctggtc cgattctgcc aggtcatctt ggtcctggcc caggagcgat aattccaaca
    16561 atgcgtatcg gtgctcaaca aagttatgaa cgttgttggc aaccgccagt ttgaacagtg
    16621 ccgtggcgct gtccgaatcc cccagactta ggtagtactt acctaaatag aagttggttt
    16681 cactgagatg ctcagcgagc gaggtgttat ccgttgcgtc cgccttgagc ctttccatta
    16741 acgtttgttc gctaatgttg cccaggtaga actcgacaat gttccatccc cactgttcct
    16801 tatccgattt ttcgaagtgc tgtttcaaca cttctttagc ctgcttctca tcgagcttct
    16861 gctcggcgag ataaagccac agactacgga aaggatcatt gggatcgtct tgataaaacg
    16921 ccagcagatc atcttgcgct aacttgtcac gaccgccgta atataatgcg atcccgcgat
    16981 tcaagtgcgc gtagttgtaa gttggatcaa gctcaagtac agaatcaaac gcttcatagg
    17041 cagcatcaaa attgcctgcc tgcgttaaat atatgcctaa gtaattgaac acttcaggca
    17101 tatccggtcg gattgccagc gcttgcgaaa aatcgttacg cgctaatgcc ctcagaccga
    17161 gactatcata caacactccg cgctcatata aaagctgtgc gcgttcgtca tcggttaaag
    17221 cccgactggc aaggatttgt tccatacgtg ccagaatcac ttcctgctgt aaagtcggtt
    17281 gcaatggtac cgcgaggact tcacttttac gccaggaagt attactgcat cctgcaagcg
    17341 taagtgctgt cgcaacgaaa caccagcgca aaaaaggctt catttcccac tcccgaagac
    17401 cacggttgaa tgaacgtcct gttcccggtt gctaacaagg cgtcctgccc ggttaaaagc
    17461 cccccgccgc agcggagggc aaatggcaac cttactcgcc ctgttcagca gccggagctt
    17521 ccggtgctgc agcaggttga gactgctcag tcgcttcttt aatgctcaga cggatacggc
    17581 cctggcgatc aacttccaga actttcaccg gtacttcctg acccatctgc aggtaatcgg
    17641 tcactttctc aacgcgtttg tcagcgattt gagagatgtg gaccagacct tctttaccgc
    17701 cgccgatggc aacaaatgcg ccaaagtcaa cgatacgggt cactttacca gtgtagacgc
    17761 ggcccacttc gatttctgca gtgatctctt cgatacgacg aatagcatgt ttcgctttct
    17821 cgccgtcggt cgctgcgatc ttcacagtac cgtcatcttc gatttcgatg gtagtgccag
    17881 tttcttcggt cagggcacgg ataacagagc cgcctttacc gataacgtct ttgatcttgt
    17941 ccgggttgat cttgatggta tggatacgcg gtgcgaactc agagatatcg ccacgcggcg
    18001 cgttgatcgc ctgttccatt acgcccagga tatgcagacg cgcaccttta gcctggttca
    18061 gcgcaacctg catgatctct ttggtgatac cttcaatttt gatatccatc tgcaatgcag
    18121 agataccctc gcgggaaccc gcaactttga agtccatatc gcccaggtga tcttcgtcgc
    18181 ccaaaatgtc agacagtaca acgtagttgt cgccttcttt caccagaccc attgcgatac
    18241 ccgcaacggc agctttgatc ggcacacctg cgtccatcag cgccagagac gcgccgcaca
    18301 cggaagccat agaagaggaa ccgttggatt cagtgatttc agacacaaca cgtacggtgt
    18361 acgggaattt gtccatatcc ggcatgactg ccagcacgct gcgcttcgcc agacgaccgt
    18421 gaccaatttc acgacgcttc ggagaaccga ccatgccggt ttcgcctacg gagtacggag
    18481 ggaagttgta gtggaacagg aaggtatcgg tacgttcgcc catcagttca tcaagaacct
    18541 gcgcgtcacg agcagtaccc agcgttgcgg taaccagcgc ctgcgtttca ccacgggtga
    18601 acagcgcaga accgtgagta cgcggcagca cgccagtacg cacatccaga ccacggatca
    18661 tatctttttc acgaccgtcg atacgcggtt cgcctgccag tacgcggcta cgaacaacgt
    18721 ttttctcgat agcgtgcaga atttcaccca gttcgttttc gtccagggtt tcgtcttcag
    18781 caagcagcgt cgcgatggtt tcagatttga tgacatcaac ctgcgcataa cgctcttgtt
    18841 tgtcggtgat gcggtaagca tcgctcaggc gagcttcagc cagtgcagca acgcgcgcgt
    18901 tcagcgcttc gtttaccggc tccggctgcc agtcccaacg tggtttaccg gcttctttca
    18961 ccagttcatt gatgttctga ataacaacct gctgttgttc atgaccgaac actactgcgc
    19021 ccagcatctg gtcttcgctc agcagttcag cttcagattc aaccatcaga acagcggctt
    19081 cagtaccggc aacaaccaga tccagtttgc tctctttcag ctcgtcctga gtcgggttca
    19141 gtacgtactg gtcattgatg taacctacgc gagcagcacc aatcggacca ttgaacggaa
    19201 taccagacag agacagtgct gcggaagcac caatcatcgc gacgatatcc gggttaactt
    19261 gcgggttaac agaaaccacg gtggcgataa cctgaacttc gttgacgaag ccttccggga
    19321 acagcgggcg aatcgggcgg tcaatcagac gcgcgatcag ggtttcgcct tcgcttggac
    19381 gaccttcacg acggaagaag ctacccggga tacgaccagc agcgtaggta cgctcctgat
    19441 agttaacggt cagtgggaag aagtcctgac ctggtttggc ttttttctgg ccaacaacgg
    19501 taacaaatac cgcggtgtca tccatgctaa ccataacagc ggcagtagcc tgacgagcca
    19561 tcatgccggt ttccagagtc acggtgtgtt ggccgtactg gaatttacga acgatcggat
    19621 taagcaatgt aatatccttt ctctttctta gacagtacct tacggcactg gtgttaatac
    19681 ccgatcttct gcgcatcctc gcgactaatg acaaccctaa cccagctcta tgtgggtaaa
    19741 gcctctcatt agccgcgcga acctctgcaa cggaagatca ttcatagcaa caatacatta
    19801 gtttccagtg aattgctgcc gtcagcttga aaaaaggggc cactcaggcc cccttttctg
    19861 aaactcggca ggaattagcg acgcagaccc aggcgctcga tgagctgggt gtaacgtgct
    19921 acgtctttac gtttcaggta gtcgagcagt ttacgacgct gagaaaccat gcgcagcaga
    19981 ccacgacggc tgtggtgatc ttttttgtgc tctgcaaagt ggccctgcag gtggttgatc
    20041 tgtgcagtca gcagtgctac ctgaacttcg gtagaaccgg tgtcgtttgc gtcacgacca
    20101 aactcagaaa cgattttagc tgttgcttca gtacttagag acattttaaa actccaaagt
    20161 atatagaatg aaaggacgcc gatctctaat tcagcgatcc cagtgtacgt tacgcaaagt
    20221 gttaaacaat ttacgcgacg ttaagcggca gtattctact cgtagcgact tgttatcgca
    20281 agacggttaa cattacgccg ggtattcaac caccaggcga cgaggcgcga cgcggccttc
    20341 atcgtcaatt tcgcccatac cgataaactt gccgttctca ccttctgtaa cgcgaaccag
    20401 tccttccagc ggcgcaccgg atgtacgaac tgggttacca tttttgaagt aaacagaaga
    20461 cgttaacgga agattcacca ccgggtagtc cgaagctgga ctgtccattg gcatcagtaa
    20521 tggatcaagt aactccgcag ccggaatatc ctgctgttcg gcttgttcga ccagttcacg
    20581 cagatgttcc agggtcacca tccgttcaac cggatattta cttaccgcca gacgacgcag
    20641 gtaaataaca tgcgcgccac aaccgagttt ttcacccagg tcatcaatga tggtgcggat
    20701 ataagtgcct tttgagcagt gaatttccag ctccagctca ttgccttcgt ggcgaataaa
    20761 cagcaattca taaatggtaa tcgggcgcgc ttcacgcggt acttcaatgc cctgacgcgc
    20821 atattcgtac agttttttac cctgatattt cagtgctgaa tacatcgaag ggatctgttc
    20881 gatatcgcca cggaaagtat ccagcgccgc cgccagttgc tctgcgctaa aggttaccgg
    20941 acgttcttca acgatttgtc cgtcggcatc agaagtatcg gtacgctgtc caagacgcgc
    21001 aatgacccga tagcgtttgt cggagtccag cagatactgg gaaaacttcg tcgcttcccc
    21061 gaagcaaatt ggcaacatgc cagttgccag cgggtccagc gcaccggtat gcccggcacg
    21121 gttggcgtta taaatccgtt tcactttttg cagcgcatca ttgctggaca taccctgcgg
    21181 tttatccagc aacaaaacgc cgttaatgtc gcgaccgcga cgacgaggac gactcattag
    21241 tcctccttgc tgtcgtccgg gttaacacga cgttcttcgt catgtttgac cacgctggtc
    21301 accaggtttg acatgcgcat cccttcaacc agagagttgt cgtagaagaa ggtcagttcc
    21361 ggcacgatac gcaggcgcat cgctttcccc agcaggctgc ggatgaaacc agaagcttct
    21421 tgcaacgctt tgatgcccgc tttaaccgcg tcttcatctt tgtcgttgag gaacgtcaca
    21481 tatactttgg catacgccag gtcgcgagac atttcgacac cggaaacggt ggtcatcatg
    21541 cccaggcgag gatctttaat ttcacgctgc aggatgagag cgatctcttt ttgcatttcc
    21601 tgcgctacgc gctgcgggcg accaaattct ttcgccataa taaattctcc tgacaaaaaa
    21661 gggactgtta gccccttttt aaaattaatt tcaggtggaa gggctgttca cgttggcctg
    21721 ataagacgcg ccagcgtcac atcaggcaat ccatgccgga tgcggcgtaa acgccttatc
    21781 ccgcatggaa ccctaaaaac cttaagcgat ggtacgttgg atctcgatga tttcgaatac
    21841 ttcgatcaca tcgccagtgc ggacgtcgtt gtagttctta acgccgatac cacattccat
    21901 accgttacgg acttcgttaa cgtcatcttt gaagcggcgc agggactcca gctcgccttc
    21961 gtagataacc acgttgtcac gcagaacgcg gatcgggttg tgacgtttaa ccacaccttc
    22021 ggtaaccata cagcctgcga tggcaccaaa tttcggcgat ttgaacacgt cacgaacttc
    22081 cgccagaccg ataatctgct gtttcagttc cggagacagc ataccgctca tcgccgcttt
    22141 cacttcgtca atcaggttat agatgacgga gtagtaacgc agatctaggc tttccgcttc
    22201 aatcacttta cgtgcagagg catcagcacg tacgttgaag ccaaccagga tggcgttgga
    22261 cgccgcagcc agggtggcgt cggtttcggt gataccacct acgccagaac cgatgatctt
    22321 cactttaact tcgtcagtag acagtttcag caaggagtcg gagatcgctt ccacagaacc
    22381 ctgtacgtct gccttcagga cgatgttcac ttcgtgaact tcgccttcgg tcatgttggc
    22441 gaacatgttc tcgagtttag atttctgctg acgcgccagt ttaacttcgc ggaatttacc
    22501 ctgacgatac agtgcaactt cacgcgcttt cttctcgtca cgtacaacgg taacttcatc
    22561 acccgcagcc ggtacgccgg acaggccgag gatttccacc ggaatggacg gacccgcttc
    22621 cagcacttcc tgacccagtt cgttacgcat cgcacgaaca cgaccgtatt cgaagccaca
    22681 cagaacgata tcgcccttgt gcagagtacc ttcacgtacc agaacggtag caaccggacc
    22741 acgaccttta tcgaggaagg attcgataac cgcaccgctc gccatacctt tacgtaccgc
    22801 tttcagctcc agaacttccg cctgcagcag gatagcgtcc agcagttcat cgatgccggt
    22861 acccgctttc gcagatacgt gtacgaactg gctttcaccg ccccactctt ctggcaggat
    22921 gccatactgg gagagttcgt tcttaacacg atccggatcg gcttctggtt tatcaatctt
    22981 gttcactgca accaccaccg gtacctgcgc cgctttcgcg tgctggattg cttcgatggt
    23041 ctgcggcatc acgccgtcgt cggcagcaac aaccaggact acgatgtccg ttgcctgcgc
    23101 accacgagca cgcattgaag taaacgcggc gtgccctggg gtgtccagga aggtgatcat
    23161 gccgttttca gtttcaacgt ggtatgcacc aatgtgctgg gtaatgccgc ccgcttcgcc
    23221 agaggccact ttcgttgaac gaatgtagtc cagcagagag gttttaccgt ggtcaacgtg
    23281 acccatgatg gtcacaaccg gcgcgcgcgg ttcagccgca gcccccgtgt cacggtcgct
    23341 cattaccgcc tcttccagct cgttttcacg acgcaggata actttgtggc ccatctcttc
    23401 agcaaccagc tgtgcggttt cctgatcgat aacctggttg atggttgcca ttgcgcccag
    23461 tttcatcatc gctttgatga cctgagagcc tttaaccgcc atcttgttcg ccagttcgcc
    23521 aacggtgatg gtttcgccga tcacaacgtc acggttaacg gcctgagctg gcttctggaa
    23581 gccttgctgc agggaagaac ctttacgttt tccgccttta cctccacgta ctgctgcgcg
    23641 agcttcttca cgatcagctt ttgattcagc gtgtttgttg cctttcttcg gacgcgctgc
    23701 tttcgcgtta cgaccacggc cacggccgcc ttcgacttca cgatcgcttt cgtcttctgc
    23761 ctggcgagca tgttgagaag tagtgacgtg ataatcgctg gaatcttcag tcggttccgc
    23821 gttatcagtc catttgtttt cttccgccat acgacgtgct tcttcagcaa cgcgacgtgc
    23881 ttcttcttcg agtttacgac gcgcttcttc ttcagcttta cgcttgagct ctgcagcttc
    23941 ctgctcacgg cgggcttttt cagcctgggc gtttttagtc atatcgtctt gttgattgct
    24001 cactttgtct ttttccgcag cttcacgttt cgcttgttca gcagcttcac gcttagcttg
    24061 ttctgcggcc tcacgttcag ctttttgttg cgcctcgcgt ttagccgatt cttctgcctc
    24121 acgacgggct tgctcttccg ctgcaaggcg ttcagcctct tgcggatcgc gtttcacaaa
    24181 ggtgcgtttc ttgcggactt cgatttgtac cgatttgctt tttccaccgg taccaggaat
    24241 gttaagggtg ctgcgtgttt tacgttgcag cgtcaatttg tccgggcctg aatttttctg
    24301 attcaggtgg tcaatcaaag tctgtttctc ttgtgcggac acagagtcgt cagcagactt
    24361 ccggatacct gcatcagcaa attgctgtac caggcgttcc acggaggtct gtcgctctgc
    24421 ggccagcgtt ttaatcgtta catctgtcat gctgttcctt cctgctacag tttattacgc
    24481 ttcgtcaccg aaccagcaaa tattacgggc agccataatc agtgctccgg ctttttcgtc
    24541 ggtcacccct tcgatatcag ccagatcatc aatgccctgt tcggcgagat cttccagcgt
    24601 acaaacgcca cgggcggcca gtttgaatgc caaatcacga tctatccctt caaggttcag
    24661 cagatcgtca gccggtttgt tatcaccgag gctttcttcc tgggcctgtg caatggtggc
    24721 cagtgcattt ttagcacgct cgcgcagtgc ttcaacggtc ggctcatcaa ggccttcgat
    24781 ttccaacagc tctttcatcg gcacataggc cagttcttcc agcgtcgaga agccttcttc
    24841 taccagaaca gtcgagaagt cttcgtcgat gtcgagatat ttggtgaagg tgtcgatcgc
    24901 tgcgtgcgct tccgcctgat gcttagcctg caggtcgtca acggtcatca cgttgagttc
    24961 ccaaccgctc agctgcgaag ccagacgcac gttctgaccg ttacggccaa tcgcctgcgc
    25021 caggttaccg gcttcaacgg cgatatccat ggtgtgttta tcttcatcca ccacgataga
    25081 agcaacgtct gccggtgcca ttgcgttaat cacaaactgc gccgggttat catcccacag
    25141 gacgatatcg atacgctcgc cgcccagttc agtagaaacc gcctgaacac gcgcgccacg
    25201 catacctaca caggcaccta ccggatcgat acgtttatcg ttggttttca ccgcgatttt
    25261 cgcacgagaa cccggatcgc gagccgctgc tttaatttca atcacttctt cgccgatttc
    25321 tggcacttca atacggaaca gttcgatcag catttccggc ttggaacgag tgacgaacag
    25381 ttgcgcgcca cgcgcttccg ggcgaacgga atagagcacg ccacgaacgc ggtcgccagg
    25441 gcggaagttt tcacgcggca gcatatcttc gcgcaggatc acggcttcag cattgttgcc
    25501 cagctccaga gagatgttgt cgcggtttac ttttttcacc acgccggtga tgatttcacc
    25561 ttcgtgttca cggaactgat caaccaccat cgcacgttcg gcttcacgca ctttctgcac
    25621 gataacctgt tttgccgtct gggtagtgat acggtcaaag gtaacagact caatctgatc
    25681 ttcaacgtaa tcgcccaggt tcaggctttc atcttcataa cgtgcggctt caagggtgat
    25741 ttccttggtc ggctgggtga cttcatcaac aactaaccag cgacggaagg tgtcaaaatc
    25801 accgcttttg cgatcgatct gtacgcggac gtcgatctct tgttcatatt ttttctttgt
    25861 tgctgtcgcc agcgcgcttt ccaatgcttc gaaaatcttc tcgcgaggta gcgccttttc
    25921 attggatacg gcttcaacta cagccaaaat ttctttgttc atcgcgggct tttcacctca
    25981 tccagactat taaaagtggg gaaccaggtt cgccttctgg atattactca gcgcgaacac
    26041 ttcatcttta ccttcgacgg taactgtgat catttcaccg tctaccgctt tgataacgcc
    26101 ctgccattta cgacggtttt gtaccgccat acggagaacc agagtcacct cttctccgac
    26161 aaaacgggcg tagtgttcag ccgtgaacag tgggcgatcg agacccggtg aggagacttc
    26221 caggttataa gcaacggtga tgggatcttc aacatccagc acagcactta cctggtggct
    26281 cacatcagca caatcatcaa cattgatgcc atcttcacta tcaatataga tgcgcagtgt
    26341 ggatgtgtga ccgcgaataa attcgatgcc aaccagttca aaacccaggg cctcaactgg
    26401 cgcagtaatc atctctgtta atttttgctc taatgtggac aagcccaccc ccaagacata
    26461 aaaaaagggc ctaaagccca gttattctgt agtcagataa caaaaaaccc cgataaatcg
    26521 gggctttata taactgaacc ctataatcgc aactgcggtc tggagcactt tccagaagga
    26581 ttttttcaaa tcccactacg aaggccgaag tcttcacagt atatttgaaa aaggactcta
    26641 agggaaagtg gttgcggggg ccggatttga accgacgacc ttcgggttat gagcccgacg
    26701 agctaccagg ctgctccacc ccgcgcctga aactcggcaa attctactcg ttttgggtaa
    26761 aagatgcaaa tactgctggg atttggtgta ccgagacggg acgtaaaatc tgcaggcatt
    26821 atagtgatcc atgccacatt ttgtcaacgt ttattgctaa tcacgtgaat gaatatccag
    26881 ttcactttca tttgttgaat acttttgcct tctcctgctc tcccttaagc gcattatttt
    26941 acaaaaaaca cactaaactc ttcctgtctc cgataaaaga tgattaaatg aaaactcatt
    27001 tattttgcat aaaaattcag tgagagcgga aatccaggct catcatcagt taattaagca
    27061 gggtgttatt ttatgacgac gattctcaag catctcccgg taggtcaacg tattggtatc
    27121 gctttttctg gcggtctgga caccagtgcc gcactgctgt ggatgcgaca aaagggagcg
    27181 gttccttatg catatactgc aaacctaggc cagccagacg aagaggatta tgatgcgatc
    27241 cctcgtcgtg ccatggaata cggcgcggag aacgcacgtc tgatcgactg ccgcaaacaa
    27301 ctggtggccg aaggtattgc cgctattcag tgtggcgcat ttcataacac caccggcggc
    27361 ctgacctatt tcaacacgac gccgctgggc cgcgccgtga ctggtaccat gctggttgct
    27421 gcgatgaaag aagatggcgt gaatatctgg ggtgacggta gcacctataa aggaaacgat
    27481 atcgaacgtt tctatcgtta tggtctgctg accaatgctg aactgcagat ttacaaaccg
    27541 tggcttgata ctgactttat tgatgaactg ggcggccgtc atgagatgtc tgaatttatg
    27601 attgcctgcg gtttcgacta caaaatgtct gtcgaaaaag cctactccac agactccaac
    27661 atgcttggtg caacgcatga agcgaaggat ctggaatacc tcaactccag cgtcaaaatc
    27721 gtcaacccga ttatgggcgt gaaattctgg gatgagagcg tgaagatccc ggcagaagaa
    27781 gtcacagtac gctttgagca aggtcatccg gtggcgctga acggtaaaac ctttagcaac
    27841 gacgtagaaa tgatgctgga agctaaccgc atcggcggtc gtcacggcct gggcatgagc
    27901 gaccagattg agaaccgtat catcgaagcg aaaagccgtg gtatttacga agctccgggg
    27961 atggcactgc tgcacattgc gtatgaacgt ctgttgaccg gtattcacaa cgaagacacc
    28021 attgagcagt atcacgcgca tggtcgtcag ttgggccgtc tactgtacca ggggcgttgg
    28081 tttgactccc aggcgctgat gctgcgtgac tctctgcaac gctgggttgc cagccagatc
    28141 actggtgaag ttaccctgga gctgcgccgt gggaacgatt attcaatcct gaataccgtc
    28201 tcagagaacc tgacctacaa gccagagcgt ctgacgatgg aaaaaggcga ctcggtgttc
    28261 tcgccagatg atcgtattgg tcaattgacc atgcgtaatc tggatatcac tgatacccga
    28321 gagaaacttt ttggttatgc caaaactggc ctgctctcct cctctgccac ttcaggcgtg
    28381 ccgcaggtgg agaatatgga aaacaaaggc cagtaattcg acatcaaccc tgcccttcag
    28441 ggggggggtt accttgctta tcctctgtaa actcctttct gaacatcaca taatcgaaaa
    28501 catcatggtt tgcatccaca acccgtgagt ctcctttata gtgtgcaatc acttcctcaa
    28561 gcgtttgcgg ctgttccggc tttgttaccc ccatccacgc attaataagg taattattgt
    28621 aagctgtcga aaagatgttg ttttccgttg tgcgatccac gccatcccca agaacggggc
    28681 tataccagat aaacatcggg acatgatatg cctgctggct agcctccctg cctccatgaa
    28741 aatagacgtt cttcttcgta gggtcacgtt ccaggccgtg gtcggcaaaa tacatgaccg
    28801 aggcgcggcg atcttttaat aattcaaaaa cctgacctag caaactatct gtgtaatgaa
    28861 tggagttgtc atagcaggca tcctgatcgt cctgcggttg aaacacggcg ctggattgcg
    28921 gataggcgct acaagccggt tcatggcttc catttaaatg aagaacaatc agtttctttt
    28981 gctgcgtatt ttgctgtaat gcttgcgata aatgcggcaa caacaattca tcaaatcctc
    29041 tgacataaac tgtttccatg gcgcgcatgg cgatactggt aactgcagta ccattctgcc
    29101 gaaaagcgga ttgcgagctt agccagaaag tctgaaatcc tgcttgatta gccatattaa
    29161 taatgttgtc ggggtaatta tgaatgtcat gactcaaaac agaatcagca gttaaagaaa
    29221 ggggaaccga cagcgcagtg taaggtgcgc tgcttattgc ttgattaaac agtttgatct
    29281 gttttctttg tgcttcaact tgcggtgtcg tagagcgtgt atatccatac aaagacatat
    29341 tgtcgacacg tacagactcc cccacaatca acacgtaggt atcaatacct gtatccctga
    29401 ctgataattg aaaatacggc accgtgtttg caattgagag taatctttga tgctctttcg
    29461 ctgctaaagc aaaatagttg agattgaaaa acggcgtata ggtagcaaat cgcgacgcta
    29521 gtatatatgg actgaacgtt tttttatttt ttgcatcttt ataagcaaat tgacaagcgg
    29581 aaaataaact gcccgaaatg acaatcagca ataatattcc tgtcactttt tttgtcggca
    29641 aggaaacatc atattttatt attactgccc aaaaaagaag ggataaaaaa gcaaaggcac
    29701 atagataagg gatatacatc cccagcattt tgacaacttc atccggatca ctctggagca
    29761 cactaatcgc aaatccatca ttgaaagttg taccaaaggt acaccatgaa tagaggctaa
    29821 tactcatgtc tgccgcagtc agtaacacaa atggtgcggc aacaagcagg cgaagcggaa
    29881 gggatgatat acgttttaca accaggatgg tcaggacgaa gaacagcaat cgctgaacca
    29941 tatgcgcgcc ggaagaggcg actaagttcg taataccaaa aacaataaca caaagataca
    30001 acaaccaatg agatttaaaa cttctagcaa atttgttgaa tactgtcatc cagaaatcca
    30061 agttcacaac caatataaac aattatcaac aatgttcaat tttatatcag acatgttttt
    30121 caactctgat ccatatcaga ccgaaacact gttagttttt tctttttaaa tgatgcggcc
    30181 attgcgttct cagtaattaa ataacattat caatgcgtct ctaaacatta cgcagaacgt
    30241 agccatcagt agttatactg acaggagcag gatggtttat cgagaagtaa acatatccgt
    30301 attcgtaccg gaactggagt tgacacaata aagtgccaat tatgtcagca gaagggaaaa
    30361 gtttataact aaggcgtacc ggcaccatcg tttcaaggta ccagctacga gtaaagcaac
    30421 tggacgagat acagatacct gacaaccatt cctcagacca ggaccaaaac gaaaaaagac
    30481 gcttttcagc gtctcttttc tggagtttgg taccgaggac gggacttgaa cccgtaagcc
    30541 ctattgggca ctaccacctc aaggtagcgt gtctaccaat tccaccacct cggcacggat
    30601 actacttttt agttcgggat atcgctggtc ggcttagccg gagcagctgg ttgagtttgt
    30661 tcggttttcg ccggtgcact cagattgtcc cattcgctac ctttattggt tttgttgctg
    30721 ttgatgttac ccagcaccag actgatgatg aagaataacg ttgccagcag cgccgtcatg
    30781 cgggtcatga agttaccaga accacttgaa ccaaacagcg tagcggaagc gcctgctccg
    30841 aaggaggctc ccatatcagc gcctttacct tgctgcagca tgatcagacc aacaaggcca
    30901 attgccacaa taaggaaaac taccaaaaga gcttcgtaca taatcaacct gttccttgcg
    30961 gagttgccgc gtaccaatgc ttcaaccaat aaagcgggag tttttaatct ttcccactga
    31021 agcgggtgtg aatactaacc aaagcgaatg accttcgcaa gggcaatttt atcgcattgt
    31081 atcaactgcg gaaaaaaaca gcaaaaccga tgtgttcgct gagaaaaaag gcgacaatcg
    31141 ccgccttttt agccagtcat ttatcgtttt aaacggcttt tactgcatcg gcgatgcggt
    31201 gtgcaaattc agtcacctgc gcttcgtctt cgccttccac catcacgcga attaacggtt
    31261 cggtgccgga tttacgcagc aacacgcgtc cacggttgcc cagcgcagct tcaacctctg
    31321 cggtcacggc tttaactgac tcatgctcaa gcggatcgcc gctacctgcg gtgtaacgca
    31381 cgttaaccag aatctgcggg aacattttca tgccgctgca aaggtcgtgc aggctcatat
    31441 ggttacgtgc catcgccgcc agcacctgca agccagcaac gatgccgtca ccggtagtgg
    31501 ttttatccag caggatcaca tgaccggaat tctctgcacc gatacgccag cctttctcct
    31561 gcattttttc cagtacgtag cggtcaccca ctttcgcgcg cgcaaatgga atacctaact
    31621 gtttcagcgc cagctcaagc cccatgttac tcatcaatgt acccacagca ccaccacgca
    31681 gctggccctg acgaagacct tcacgcgcga tgatatacat gatctgatca ccatcgactt
    31741 tattgccttc atggtcaacc ataatcacgc gatcgccgtc gccgtcgaag gcaataccga
    31801 gatccgcttt ttcagccagc acacgagcct ggagcgcgcg aacgtcggta gcccccactt
    31861 cggcattgat gtttacaccg ttcggctcac aaccgatagc gataacgttc gcccccagtt
    31921 cacgcagcac gttcggcgcg atgtgataag tcgcaccgtt tgcgcaatcc accacaatct
    31981 tcagttcgct gaggctaagt tcgttcggga acgtggcttt gcaaaactcg atatagcgac
    32041 ccgcagcatc aacgatacgg ctggctttac ccagttctgc cgaatcaaca cagctgatct
    32101 ctttttccat ttccgcttcg atggcctctt ctaccgcatc cggtagtttg gtgccgtcga
    32161 tagagaaaaa tttaatgcca ttatcgtaga acgggttatg cgatgcagat atcacaattc
    32221 cggcctctgc gcggaaggta cgcgtcagat aagccacggc cggtgttggc atcgggccag
    32281 tgaagagtgc ggaaaggccc gctgccgcca gacccgcttc cagtgctgac tccagcatat
    32341 agccagaaat acgcgtgtct ttactaataa tgatcttacg ggagccgtgg cgcgccagca
    32401 ctttacccgc ggcccaaccc agcttaagca caaaatcagg tgtgatcggc gcatccccta
    32461 cacgaccacg aatcccatcg gtaccgaaat atttacgatt actcatagcg tttgttttcc
    32521 tttgcagaca gagtggcttc caccacccgc atcgcttcta cggtttcttt gacgtcatga
    32581 acacgaataa tgtgcgcgcc ttgcattgcg gcaatgaccg cacaggccag actaccgctc
    32641 aggcgctcgg acggccccac gttcagcagc tgcccaatca tcgattttcg tgacatacct
    32701 accaacagcg gcaggttgaa atggtgaaat tcagccaggc gcgccagtaa tgaatagtta
    32761 tgggagagat ttttaccgaa accgaatccg gggtcgagca acaatttctc ttttgcgata
    32821 cccgcctgct cgcaacgtgc tatttgctca ataaagtagc gattcacttc tgcaaagaca
    32881 tcgtcatact tcggagcttc ctgcatggtt tttggatttc cctgcatatg catcagacaa
    32941 accggtaaac cggtttctgc cgccgcctcc agagcgccag gttcggaaag ggagcggata
    33001 tcgttaataa tgtgagcgcc aactttcgct gactcacgga tgacttctgg tttggatgta
    33061 tctaccgaga gccagacttc gaagcgttga gcaattgcct caaccacagg aataacacgt
    33121 tgcaactctt cttcaacgct aacttccgcc gcccctgggc gcgtggactc gccaccaacg
    33181 tcaatgatcg tcgcgccagc gttgatcatc agattcgcat gtttcaccgc atctatcagc
    33241 gagttatgcg tgccaccatc cgaaaaggaa tcaggcgtga cgttgaggat ccccattacg
    33301 tgaggatggc taaggtccag tgaagtgccc tgggcaaaga gtttcatggt gttatccctg
    33361 gtatgaattg ataagaaaaa accccggagc acgccccggg gttttcggta caaatacagt
    33421 catctgatgc gggaacttac ttgtcgccta actgctctga catggtgtta cccgggttcg
    33481 gcgtacgcgg ttcatcaacc ggacgaggag cctttggact accattgtcg ccagaattgt
    33541 tagaagcgcc tggttcttcc cagcccgctg gcggacgtac atcgcgacgt gccatcaggt
    33601 catcaatctg cggtgcgtcg atagtctcat atttcatgag agcatctttc atcgcatgca
    33661 gaatatccat attgtcggtc agaagctgac gcgcacgatt atagttacgc tcaatcagtg
    33721 ctttcacttc ctggtcgatg atacgtgcag tttcatcgga catatgtttc gctttcgcta
    33781 cgctacggcc gaggaacact tcaccttctt cttccgcgta cagcagtgga cccaatttct
    33841 cagagaagcc ccactgagtc accatattac gcgccaggtt ggtcgcaact ttaatatcgt
    33901 tggacgcacc ggtagaaaca tgttccggcc cgtagatgat ctcttctgcc agacgaccac
    33961 cgtacagcgt agaaatctgg ctttccagtt tctgacggct ggcgctgatt gcgtcgccct
    34021 caggcaagaa gaaagtcaca cccagcgcac gaccgcgtgg gataatcgtc actttgtgca
    34081 ccggatcgtg ttccggcacc aagcgaccga taatcgcatg acccgcttcg tggtaagccg
    34141 tcgattcttt ctgcgcttcc gtcatcacca tggagcgacg ttccgcaccc atcatgattt
    34201 tatctttcgc tttttcgaac tcaatcatcg acacaacgcg tttgttgcca cgagcagcga
    34261 acagtgccgc ttcgtttacc aggttcgcca ggtcagcacc ggagaaacca ggagtaccac
    34321 gggcaatgat tgccgcgtcg atatcgggtg ccaatggtac gcgacgcatg tgaactttca
    34381 ggatctgctc acgaccgcga acatctggca agccaaccac aacctgacgg tcgaaacggc
    34441 caggacgcag cagggctggg tcaagaacgt ccggacggtt agtcgcggcg ataacgatga
    34501 taccttcgtt accttcgaag ccatccatct caaccagcat ctggttcaga gtctgttcac
    34561 gttcatcgtg accaccgccc agaccagcgc cacgctggcg gcctacggcg tcgatttcat
    34621 cgataaagat gatgcacggt gccgctttct tcgcctgttc gaacatgtca cgaacacggg
    34681 atgcacccac accgacgaac atttctacga agtcagaacc ggagatagta aagaacggaa
    34741 ctttcgcttc gcctgcaatc gctttcgcca gcaacgtttt accggtaccc ggaggaccga
    34801 ccatcaggac gcctttcggg atcttaccgc cgagtttctg gaagcggctc ggctcgcgca
    34861 gatactctac cagttcagca acttcttctt ttgcttcgtc gcagcccgca acgtcagcaa
    34921 aggtcgtttt gatctgatct tccgtcagca tgcgcgcttt gctcttacca aacgacatgg
    34981 cacctttgcc accgccgccc tgcatttgac gcatgaagaa gatccagaca ccaatcagca
    35041 acagcatcgg gaaccaagag atgaagatag aagccagcag gcttggttct tcaggcggtt
    35101 caccgacaac cttgacgttc ttggtcaaca ggttatccag taatttcgga tcctgaaccg
    35161 gaatgtaagt ggtataacgg ttactatctt tcttggtaac gttgatttca cgtccgttga
    35221 tacgcgcttc acgaacctgg tcgttattga cctcttgtag gaaggtagag taatccacct
    35281 tacggccatt agactcgctg ggcccaaagc tctggaatac tgacatcagc acaacggcaa
    35341 tgaccagcca gagtattagg tttttcgcca tgtcactcaa gggattaacc tcttattaca
    35401 actgtgttaa aaacagcgtc aggatactct atatccagca tctttcaaac tttcgtctga
    35461 aatctcccgg ttagggttta cgcccggtcg ctacaatata cacttcccgc gaacgtgcac
    35521 gagaagagtc cggcttacga actttgacct tcgtaaacag ggagcgaatt tcccttagat
    35581 actcatcgaa accttcgccc tggaacacct tcactacaaa actgccacct ggcgctaata
    35641 catcacgaca catttctaac gccagttcca ccagatacat ggcacggggg atatccaccg
    35701 ccggtgttcc gctcatgttt ggtgccatat cggacatgac aacctggact ttgctgtcgc
    35761 caacgcgctc cagcagtgct ttcatcacca gttcatcacg aaaatcgccc tgaagaaagt
    35821 ccacaccaac gataggatcc ataggtaaaa gatcgcaagc gatgatgcgg cctttgccgc
    35881 caatttgggt gaccacatat tgtgaccaac cacccggagc agcaccaagg tcgacaaccg
    35941 tcattcccgg tttaaagagt ttgtcacttt gctgtatttc atcaagttta aaccaggcac
    36001 gggaacgtaa cccctttttc tgtgcctgtt gaacatattt atcgctaaag tgttcctgaa
    36061 gccagcggct ggagctggca gaacgcttct tacctgtcat ttaactttcc catggggata
    36121 actcatcgta accaattgcg taaattttta cgcgcctatt tggtgatata tgggagatgg
    36181 cggtagaatg acccgttttc aatcccaacg taagcaaaaa atacgatgaa tctgagtact
    36241 aaacaaaaac agcacctgaa aggtctggca catccgctca agccagttgt tctgcttggc
    36301 agtaatggtt tgaccgaagg ggtgctggcc gagattgaac aagcgttaga gcaccatgaa
    36361 ctcatcaagg tgaaaatcgc caccgaagat cgcgaaacaa aaaccttgat cgtggaagct
    36421 atcgtgcgcg aaaccggcgc ctgtaatgta caggtcatcg gtaaaacgct ggtgctttat
    36481 cgcccaacta aagaacgtaa aatctcgctg ccacgctaag attatcctaa agttacacac
    36541 atttgctgtg taaaacgagg ggttttccgc aggcaggaga gcaaaatgcc acgctctgtt
    36601 cgttgataaa aggccgcata gcggcctttt tcctttcttt acaatacatc aacatcttga
    36661 tgtattgggt aattcttaca ggtattccac cttaattact tcaaattcta cttcgccgcc
    36721 cggcgttttg atgaccacaa catcatcttc ttctttgccg atcaggccac gagcaatagg
    36781 cgagtttaca gaaatcaggt tttgtttaaa gtcagcttcg tcatcgccaa cgatgcgata
    36841 agtctgttct tcgtcagaat ccagattcag caccgttacg gtagcaccaa aaataacgcg
    36901 cccattgttg ggcattttgg tgacatcaat cacctgcgcg ttcgacagct tggcttcgat
    36961 gtctttaata cggccttcgc agaaaccctg ctgttcacga gctgcgtggt attcggcgtt
    37021 ttctttcagg tcgccatgct cacgcgcttc cgcgatagca gcaatgattt caggacggcg
    37081 cacagatttc agaaaatcca gctcttcgcg taatttttca gcgccgcgta aggtcatcgg
    37141 aatagcttgc atttgttata cctcttgaat attcctgatg gggcaagtct tcacccaccc
    37201 cggctgttcg gcctgcccgg cataactcct caccgggacc agaagcaaaa aaataccgac
    37261 ccgggtacaa gtcccaggtc agctacaatt cacattttga tagtcatttt accctgaagt
    37321 tcccgaaggg tcatcgttta ctttataggg cgttgcgccg tagtatgacg gctcgattcc
    37381 aggttgttag cgcgagatta tgcgattttc cagatttatc atcggattga ccagctgtat
    37441 agcgttcagt gttcaggccg cgaatgttga tgagtacgtt actcaactcc ccgctggtgc
    37501 caaccttgcc ctgatggtgc aaaaagtcgg cgcgtcggcc cccgctattg attaccacag
    37561 tcagcagatg gcgctgcccg ccagtacgca gaaagtgatt actgcgctgg cggcgttgat
    37621 tcaactcggc cccgattttc gtttcaccac gacgcttgaa accaaaggca atgtggaaaa
    37681 cggcgtactt aagggtgact tagtggcgcg atttggtgcc gatccgacgt taaaacgtca
    37741 ggatattcgc aatatgatcg cgactttgaa aaaatctggc gtcaaccaaa tcgatggcaa
    37801 tgtgttgata gatacctcca ttttcgccag ccacgataaa gcccccggct ggccatggaa
    37861 tgacatgaca caatgcttta gcgctccgcc tgccgctgcc atagttgacc gcaactgttt
    37921 ctccgtctcg ctctacagtg ccccaaaacc tggtgatatg gcttttatac gcgtggcatc
    37981 ttattacccc gttacgatgt tcagccaggt acgcaccctc ccccgaggtt ctgccgaagc
    38041 gcaatactgc gaactggatg tcataccagg cgacctgaac cgctttacgc tgacgggatg
    38101 cctgccacaa cgttctgagc cgctcccgtt ggcttttgcc gtgcaggatg gagccagcta
    38161 tgccggggca attctgaaag atgagttaaa acaggcaggt atcacctgga gcggaacact
    38221 gctgcgccag actcaggtta acgaacctgg aacggtagtt gccagtaaac agtcggcccc
    38281 gttgcacgat ctgcttaaga ttatgctgaa aaagtcggac aacatgatcg ccgatacggt
    38341 tttccgcatg ataggccatg cgcgcttcaa tgtgcctgga acatggcggg ccgggtcgga
    38401 cgccgtgcgt cagatcctgc gccagcaagc cggtgtcgat attggaaaca ccattattgc
    38461 cgatggttca gggctttcgc ggcataacct gattgccccc gccaccatga tgcaggtgtt
    38521 gcaatacatt gcccaacacg acaatgaact taactttatc tccatgctgc cgctggcggg
    38581 ctatgatggc tctttgcagt accgtgcagg tctgcatcag gcgggcgtgg atggaaaagt
    38641 ctcagcgaaa accggttcgt tgcagggggt atataacctg gcgggattca ttaccacagc
    38701 gagcgggcaa cgaatggcgt ttgtacaata tctttctggc tatgcagtag aaccggcgga
    38761 tcagcgtaat cgccgtattc cgttagtgcg ttttgaaagc cgtttgtata aagatattta
    38821 tcagaacaat tagtcaaaaa gaaaccccgg cacatggccg gggttttgct gattaacgct
    38881 tgtaaatgaa ctcaacgcct tcttcgtcgt cttcgtccca gtcatcatcc cagtcctcat
    38941 catcctcttc agcaatctct tcaagctgct ggcgatgata atcatcccac atgaattcga
    39001 ctttctctgg ctgtttcgct tcttcagcct gcacgaccgg gttttcaatg ataaaggtca
    39061 tcacatccca gcagagatct ttcacgccca gtccactcgc cgcagagatc agataatatt
    39121 tatcttccca gcccagcgct tcagcaatcg ctttcgcttt ctcttcagct tctgccttat
    39181 ccagcagatc gatcttattg aacactaacc aacgcggttt cgccgccaga tcctggctgt
    39241 atttttccag ctcgctgata ataatacgcg cgttttcaac cggatcggtg ccgtcaatcg
    39301 gatcgatatc gatgaggtgc aacaggacgc ggcaacgttc caggtgcttc aggaagcgga
    39361 tacccagacc tgcgccttcc gcagcgcctt caatcagtcc tggaatatcg gcaacaacga
    39421 agctcttttc gttgtccatt cgtaccacac ccagacttgg caccagagtg gtaaacggat
    39481 aatccgccac tttcggttta gccgccgata ccgcgcgaat aaaggtcgat ttacccgcgt
    39541 ttggcatccc caacataccg acgtcagcca gcagcatcag ctccagcagc agctcacgct
    39601 tatcacccgg cgtaccgttg gttttctgcc gtggtgtacg gttaacggac gatttgaaac
    39661 gggtattgcc cagaccgtgc cagccgccct tagcaaccag cagacgctga ccgtgtttag
    39721 tcatatcgcc catggtttca ccagtaccct ggtcgattac acgcgtacct accggcactt
    39781 taatcgtcac gtctttaccg cgcttaccgg tacaatcgcg gcttgcgccg ttctgaccgc
    39841 gctctgcacg gaaagatttt tcaaaacgat aatcgataag cgtgttcagg ttttcgtcgg
    39901 cttccatcca tacgtcacca ccatcaccac cgtcgccgcc atccgggccg cctttcggaa
    39961 tatacttttc gcggcggaag ctcacgcaac cattaccgcc atcacctgca acgaccagaa
    40021 tcgatgcttc atcaacaaac ttcattttat tctccgtaaa tcattcgcct gcgcggggtt
    40081 gcgataccac cgttgtatgc ttacgtaatc cgccccaaat acgatgacca atggcggaat
    40141 acatcgcgcc cgcaaccacg acaaacgcac cgagataacc taaaaggttt aacatcggtc
    40201 tggcgaagaa atcgggccag gccagtgata aaagatctga aaaaaacagc gtaaacagtg
    40261 gggtgagcgt gatgatcgcg ctcacctgcg ctgcctgcca gcgagccatc gcttccgcca
    40321 gggcgccata tcctaccaat gtattcagtc cgcaaaaaat taaacatgca agctgccagt
    40381 ggctaagctg cgctatcact cctggctttg ccagagggaa gagcgcaatt gtacataaag
    40441 tgtacagcaa aaacaggatc tgcggtgagg ccagccgacg caataaaacc ttttgcgcca
    40501 cgccataact cacccaaacc gtcgccgcac cgaccccaaa gatgactccc caggtgtaat
    40561 cagtgagctt tgtaaatatc tcgaccagac tggtgttaaa aaacatcacc aggccgctca
    40621 ggagcatcaa tgcccctaca acctgagtgc tgcgcatttt ctctttcagg ataaatacgc
    40681 tggcaaccat catgccaact ggcgagagtt gcccaatcac ctgcgaagcg gtcggactca
    40741 ggtattgcaa ggatgagctg aacaggatga agttcccaaa cagcccggcg gtcgccactg
    40801 ccaacaaaat caaccagcgt ggcttacgaa acacgcgtaa tggcggcaac ctcttcttca
    40861 ccgcaagaat ggcacccagg ccaatactcg ccatcaagaa acggtaaaac acgattgtcg
    40921 gaggttccat cacctccagc acctgcttca ttgcgattgg caacgccccc cagcaaattg
    40981 ctgtggtgag cgccaaaaga atgccaatgc ctgcctgctg cttcataccg tattccctgc
    41041 aaagcgcatt tgcggattaa cgtcgcacca tgctcatttt taccggttat cgaatgtaaa
    41101 aagccccgca acgtgttgcg gggctttcat ccgttaccgg gacgcgaaaa acttattcag
    41161 cttcgatgct gataaattta cggtttttcg ggcctttaac ttcgaatttc actttaccgt
    41221 ctgctttagc aaacagagtg tggtcacgac cgcaacctac gttagcgcca gcgtggaatt
    41281 tggtaccacg ttgacgaacg atgatgctac ccgccagaac ggattcgcca ccgaaacgct
    41341 taacgcccag gcgtttagct tctgaatcgc gaccgttacg tgtggagccg ccagcctttt
    41401 tatgtgccat ttgaaatctc tcctcaggtc ttaggcgctg atgccagtaa ttttcacatc
    41461 agtgaaccac tgacgatggc cctgctgctt acgatagtgt ttacgacgac gaaacttaac
    41521 gattttaact ttctcgccac gaccgtgagc aacaacttca gctttgatta cgccgccatc
    41581 aacgaaagga acgccgattt tgacttcttc accgtttgcg atcatcagca cttcagcgaa
    41641 ctcaacagtt tcgccagttg cgatgtccag cttttccagg cgaacggtct gaccttcgct
    41701 tactcggtgt tgtttaccac cactttggaa aaccgcgtac atataaaact ccgcttccgc
    41761 gcacaccttt tcaatgattc agagtgcgct ataaatattc acaatagggc gcgaatatta
    41821 cgcaaaacgc acgcctttga caagtgctac agtcaataca cgaagaaaaa aaacacaact
    41881 tgtacggtaa cgtttatctg tgccattttt tcagtacaat caccctatat tcctaaccat
    41941 aaaccctaag ttgcctttgt tcacagtaag gtaatcgggg cgaaaagccc ggcttttgcg
    42001 atgaatttag aaaaaatcaa tgagttaacc gcgcaagata tggcgggtgt taatgcggca
    42061 atccttgagc agcttaattc cgacgtccaa ctgatcaatc agttaggcta ttacatcgtc
    42121 agcggcggcg gtaaacgtat tcgtccgatg attgctgtac tggctgcacg agctgttggc
    42181 tatgagggaa atgcgcatgt caccattgct gccctgatcg agtttatcca cacggcgact
    42241 ctgctacacg acgacgttgt ggatgaatca gatatgcgca ggggtaaagc taccgccaac
    42301 gctgcatttg gcaatgccgc cagcgtgctg gtaggcgatt ttatttatac ccgcgcgttc
    42361 cagatgatga ccagcctcgg ttcactcaaa gtgctggaag tcatgtcaga agctgtaaac
    42421 gtcatcgcag aaggtgaagt tctgcaactg atgaacgtta acgatccgga catcactgaa
    42481 gaaaactaca tgcgcgttat ctatagcaaa accgcgcgtc tgtttgaggc tgccgcgcag
    42541 tgttccggga ttctggctgg ctgtacgccg gaggaggaga aaggcctgca ggattatggg
    42601 cgctatctcg gcactgcttt ccagttgatc gacgatttac tcgattacaa tgccgatggc
    42661 gaacagttag gtaaaaacgt cggcgacgat ctgaacgaag gtaaaccgac gctgccgctg
    42721 ctgcatgcga tgcatcatgg cacaccagaa caggcacaga tgatccgtac cgccatcgaa
    42781 cagggtaacg gtcgccatct tctggaaccg gttctggaag caatgaacgc ttgtggatct
    42841 cttgaatgga cgcgtcagcg cgccgaggaa gaagcagaca aagccatcgc agcgttacag
    42901 gtgctcccgg acaccccttg gcgagaagca ctcatcggcc tcgcgcacat cgctgttcaa
    42961 cgcgatcgtt aatcccctcc cttcatcccg cgcagtgcgc gggatgattc cagtaaattc
    43021 cataaaaaca ctaattcagc gctataacac ctgaaaaaaa aggcgatgaa tctttatata
    43081 ttctgaatat tcacactctt tacaggagct ttttagagca ataggccatc aggagtatag
    43141 tgatgcccga cagaagaagt gttctgaatg aaagcgacta acttaaggag tgaggaaaga
    43201 tggaaagtaa tttcattgac tggcatcccc ctgacatcat tgcaggtttg cgcaagaaag
    43261 gaacgtcaat ggcggcggaa tctcgcagaa atggtttgag ttcctcaacg ctggcaaacg
    43321 cattatcgcg cccatggccg aaaggagaga tgattattgc gaaagccctg ggaactgacc
    43381 cctgggttat ctggccatca cgctaccatg atccgcagac ccatgagttt atcgacagaa
    43441 cgcagttgat gcgtagctac actaaaccga aaaaatgagt ggtctggcgg tagccccgcg
    43501 aacggggctg ccagctctca gacgattact cgcctttcac acgcttaata tttgcaccta
    43561 aagcgcgcag tttgtcttca atgcgttcgt agccacgatc gatgtgataa atacgatcaa
    43621 ccaccgtcgt cccttccgca atacagccag ccagcaccag gcttgctgat gcacgcagat
    43681 cggttgccat aacctgtgca ccagaaagtt tttcaacacc atgacaaata acggtattgc
    43741 tttcgatttc ggcatgcgcg cccatacggc taagctccgg cacatgcata aagcggtttt
    43801 caaagaccgt ttcggtgatg aatccggtcc cttctgccac caggttcaac agcgtgaact
    43861 gggcctgcat atcggtcggg aatgccggat gcggcgcggt acgtacgtta acagccttcg
    43921 gacgtttgcc atgcatatcc aggctaatcc agtcttcgcc gacttcgatg tccgctccag
    43981 cgtcacgcag tttcgccagc acggcgtcga gagtatctgg ctgcgcgtta cggcagataa
    44041 ttttgccgcg agaaatcgcc gccgccacca ggaaagtacc ggtttcgata cgatccggta
    44101 gcacgcgata gacaccgccg cctaaatgtt ccacaccttc gatgacgata cgatcggtgc
    44161 cctgaccgct aattttcgca cccagcgtaa tcaggaagtt cgcggtatcg acgatttccg
    44221 gttcacgcgc tgcgttttca ataatcgtgg taccttccgc cagggttgca gcacacatga
    44281 tggtcaccgt tgcgccaacg ctgactttat ccatcacgat atgtgcgcct ttcagacgac
    44341 catcgacgga agctttaacg taaccttctt ccagtttgat ggtcgcgcct aattgttcga
    44401 ggccagaaat gtgtagatca accggacgcg caccgatcgt acaaccgcca ggcagtgaaa
    44461 cttgcccctg accaaagcgc gctaccagcg gccccagtgc ccagatagaa gcacgcatgg
    44521 ttttaaccag atcgtaaggt gcgcagaata cattaacgtc gcgggcatca atatgcacag
    44581 aaccattacg ttctactttc gcacccagct gacttagcag cttcattgat gtatcgacgt
    44641 ctttcagttt cgggacgttc tggatctcta ccggttcttc cgccagtaga gcggcaaaaa
    44701 ggataggcag agcagcattt ttagcgccgg aaattgtgac ttcgccctgg agcttcgttg
    44761 gcccctgaac acgaaattta tccatttagt ttgttctcag ttaacaattc atatccgcta
    44821 ccggcgaatc gcccatagct caaaagccgt tcagtttgcg atcgcgcgcc cactccgcag
    44881 gggtatacgc tttgatcgac acagcatgaa tgcggttatc cgcaatatat tccatcagcg
    44941 ggccatagac cgtctgctgt tttttaaccc gactcatgcc gtcaaacaac tcacccacgg
    45001 caataacctg aaagtggctg ccatcgccgg aaacgtggac ttcctggagg gagagagcgt
    45061 tcatcaacac gctctgaatt tcattatttt ccatgggatc ttcaatcatc agttaataaa
    45121 ccagcgaaac atcttagagc aaagttgcgc tggcataaat aagcaaaaag cctcgctgat
    45181 aaatcagaca aggctcgact tgcaggcagg tttgccggac aggcggttaa cgccatatcc
    45241 ggcctgaaaa aatttaacga ggcagaacat cagcaggcaa attatacaat ttcgccaggg
    45301 tatacacttt gtcgtttacc ccctgaagcg tcacattgtt gccctgcttt ttcgccagat
    45361 cgataagctg gagcagcagt gccagtcccc ccgtatccac gcgggacaca cggctaagat
    45421 cgatgcaggt aatccccttc accgcttcct cacgcatttc ccaaagcggt agcaaaacgt
    45481 cctgatccag ctctccggat aacgccagcg tgtcacccgt ctgcatccag ctcagtgact
    45541 cgctcattat tttttctctt ccagagtgat tttctgttga gaaatcgatt tcagttgcgc
    45601 agtcaggccg tcgatacctt tggtacgcag cagcgttccc cactcgtttt gtttggtggt
    45661 gatcatactg acgccttcag caatcatgtc gtaagcctgc cagttgcccg tctgggagtt
    45721 tttacgccac tggaagtcca gacgcaccgg cgggcggcca ttcggatcaa taatggtaac
    45781 gcgaatcggc acaatggttt tatcgcccag cggctgttct ggcgcaatct gataggtttg
    45841 accatgatac atcgccagcg cctgaccgta agcctgcttc aggtactcac ggaaagcggc
    45901 aaagtaggct tcacgttgag caggggtcgc actcttgtaa tactggccca gcaccagcgc
    45961 accggcgtat ttcacctgta cgtatggcag cagttcctga tcaacaatgg tacgcagata
    46021 atccgggttg gcccgaattt gcggttgctc attcttcagg cgatcgaacg ttttctgcgc
    46081 cgcctcgtcc atcagcttat aaggattggt ctggtctgcc gcggttgccg cactcagcgg
    46141 tgcaatcacc agcaaagcga ccatcattaa acgtttaaac atgcgtcggt tctcctgaaa
    46201 ttatttcgtt gtacccacag gttcagtggt ttcattatta cctggcgcag cagctggcgc
    46261 atcgccacta ttcttattgt catcgccttt actaccgtaa aggaactgac caatgagatc
    46321 ttccagcacc atcgcagact tagtgtcctg aattgtatcg ccatccttca ggatagcagt
    46381 ccccagttcc gggtcttcaa aaccgacgtt taatgccaga tattgttccc ccagtaggcc
    46441 ggaagtacga atgctcagcg aactggtatc tggaatgtgg ttataacgtt gttcaatttc
    46501 cagcgttacg cgtggcagat aggttttcgg gtccagcgta atatccgcca cccggcccac
    46561 aacaacgcca ccaatactga ccggagagcg cgctttcagg ccgccaatgt tatcgaacgt
    46621 cgcataaagc gtgtaggtcg gttcagtacg tatggacgtc acgttcgccg ccttcaggca
    46681 aacaaacaga gccgccagca gtgctgctaa taaaaagata cccacccaaa tttcattttt
    46741 tttcgtttgc atgtactcaa ttcccaaaca tcaatgcggt cagcacaaaa tccagcccca
    46801 gaacagccag agacgagtgg acaacggtgc gagtggttgc ccggctaatc ccggcagacg
    46861 tcgggatggc gtcgtagccg ttaaacaacg aaatccacgt caccgtgatg gcgaacacca
    46921 cgctcttaat cagacagttg accagatcca tacgccagtc gacggcattt tgcattgccg
    46981 accagaagaa cccgctatca atgcctttcc aactgacgcc gaccagagat ccgccccaga
    47041 tccccacggc gacgaaaata accgtcaaca gtggtaatga aataacccca gcccagaaac
    47101 ggggagaaat aacccgacgc agcggatcca ccgccatcat ctccatactg gagagttgct
    47161 ctgtagcgcg catcaggccg atttctgcgg ttagcgccga accagcacgc ccggcaaaca
    47221 acaacgcggc aaccaccggc cccagttcac gcagtagcga taacgccacc agcataccca
    47281 gactggtttc cgcactataa gtggtcagaa ccagataacc ttgcagcccc aacaccattc
    47341 cgatgaacac gccagaaacc acaataatca gcatcgacag gacgccgaca ttatagagct
    47401 ggcgcaccag cagcggcgca tgtttgcgaa attccggttt gccgaccagc gcattgaata
    47461 acattaaccc ggcccgcccg aacgttctca gggttttaat ccctttatgt ccgagcgacg
    47521 ccagcgcatt taacagcatg agtggcttaa ctccctggta aaagatcagc gtgataatcg
    47581 ccggcaggat agcggaacgg aacaggcccg tcagctatcc cgtccagaaa ctgacgtacg
    47641 cgcggatcag gattcgcctg caacgcctgg gcactgccat gagcgacaat ttttttgtcc
    47701 gccaggatcc aggcgtgatc cgcaatactt aacacttccg gcacatcgtg agaaaccacc
    47761 acacaagtca cgcccagcgc gctgttcagc tcagaaatca gcttcaccag tacgcccatg
    47821 gtgatgggat cttgcccaac aaaaggttca tcaaacatga tgagatccgg ctccagcgca
    47881 atcgcacgcg ccagcgctgc acgccgcgcc atcccaccgg aaagttcaga aggcattagt
    47941 ttagccgctc cacgcagccc cacggcctcc agcttcatca tcaccgtact atgcaacaat
    48001 ggcgcgggaa gttgggtatg ttcgcgcagt ggataggcga cgttgtcaaa tacgttcata
    48061 tcagtgaaca acgccccgga ctgaaataac atgctcatcc gtttgcgcac tgtatacagg
    48121 cgcgaacgag acatcgccgg aatattctca ccatcgaaaa ggatctcacc atgatctggt
    48181 gcgatttgcc cgccaatcag acggagtagc gtcgttttac cgatgcccga tggccccatg
    48241 atcgccgtga tcttccctcg cggcacggtc agggaaatat tatcgaagat gcagcgattg
    48301 ccacgcgtaa aactgacatc gcgcatatcg actaaattcg ccacagactg ctccataatt
    48361 cacccttcgt cttgcgttga ttttctaagc atggcgctca atttaacctt gaacccaaca
    48421 tatttacaga atattacccg ccgtggttag cgaaagctgg catttgtttt actttttagc
    48481 cgcataaagt caaaattaag aatccgttac ggctttctga aaatcttcag cggaccggcg
    48541 agtatacctg aagaaaggac gttagatgct tttagctacg gcactgttaa ttgttggttt
    48601 acttttggtc gtttacagtg ccgaccgcct ggtttttgcc gcgtctattc tttgccgaac
    48661 ctttggcatc ccgccgctga tcatcggcat gacggtggtc agtattggta catcgttacc
    48721 agaaatcatc gtctcgcttg ccgcgtctct gcacgaacaa cgcgatttag ccgttggtac
    48781 agccctcggc tcaaacatta tcaatatttt gctgatcctc ggtctggctg cgctggttcg
    48841 tccttttacc gtccattccg atgttctgcg tcgtgaatta cccttaatgt tgttggtcag
    48901 cgtagtggcc ggttccgtac tctatgacgg acaacttagt cgcagcgatg gtatctttct
    48961 cctctttctg gctgtgctat ggctgctgtt cattgttaaa cttgcacgtc aggctgaacg
    49021 tcaggggact gacagcctga ccagagagca gcttgcagag ctgccgcgtg acggcggatt
    49081 gcccgtcgcg tttttatggc tcggcattgc gcttatcatc atgccagtgg ccacgcggat
    49141 ggtggttgat aacgccacgg tgctggcgaa ttactttgcc atcagcgagt tgacgatggg
    49201 tctgacggca attgctatcg gaaccagcct gccggaactg gcaaccgcaa tagcgggggt
    49261 tcgcaaaggt gaaaacgaca ttgctgtcgg aaatatcatt ggcgcaaaca tttttaatat
    49321 tgtcatcgtg ttgggtttac ccgcgctgat aacaccagga gagattgatc cattggcgta
    49381 cagtcgtgac tacagcgtga tgttgctggt gagcattatt tttgcgttgc tgtgctggcg
    49441 gcgctccccg caaccgggcc gtggtgtagg ggtattatta actggcggat ttatcgtatg
    49501 gctggcgatg ttgtactggt tatcgccaat actcgttgaa taactggaaa cgcattatgt
    49561 cgcacgtaga gttacaaccg ggttttgact ttcagcaagc aggtaaagaa gtcctggcga
    49621 ttgaacgtga atgcctggcg gagcttgatc aatacatcaa tcagaatttc acgcttgcct
    49681 gtgaaaagat gttctggtgt aaagggaaag ttgtcgtcat ggggatgggg aaatcggggc
    49741 acatcgggcg caaaatggcc gcaacgtttg ccagcaccgg tacaccttca tttttcgtcc
    49801 atcctggtga agccgcgcat ggtgatttag gcatggtcac cccacaggat gtggtgattg
    49861 ctatctctaa ctcaggtgaa tccagcgaaa tcacggcctt aattccagtg cttaagcgtc
    49921 ttcacgtacc gttaatctgc atcaccggtc gcccggagag cagcatggcg cgcgccgcag
    49981 atgtgcatct gtgtgttaaa gtagcgaaag aagcctgtcc gttagggctg gcaccgacca
    50041 gcagcaccac cgccacgctg gttatgggcg atgccctcgc tgtcgcgctg ttaaaagcac
    50101 gcggctttac tgctgaagat tttgcgctct cacacccagg cggcgcactg ggtcgtaaac
    50161 ttctgctgcg cgtaaacgat attatgcata cgggcgatga gatcccgcat gttaagaaaa
    50221 cggccagtct gcgtgacgca ttgctggaag ttacccgcaa aaatcttggt atgactgtca
    50281 tttgcgatga caatatgatg attgaaggca tctttaccga cggtgattta cgccgtgtct
    50341 tcgatatggg agtggatgtt cgtcagttaa gtattgccga tgtgatgacg ccggggggaa
    50401 tacgcgtgcg ccctggcatt ctggccgttg aggcactgaa cttaatgcag tcccgccata
    50461 tcacctccgt gatggttgcc gatggcgacc atttactcgg tgtgttacat atgcatgatt
    50521 tactgcgtgc aggcgtagtg taaagattca aggataaaca acaatgagca aagcaggtgc
    50581 gtcgcttgcg acctgttacg gccctgtcag cgccgacgtt atagcaaaag cagagaacat
    50641 tcgtctgctg atcctcgacg ttgatggcgt actgtcagat ggcctgattt atatgggcaa
    50701 taacggcgaa gagctgaaag cgttcaatgt tcgtgacggt tatggcattc gttgtgcgct
    50761 cacctctgat attgaagtcg ctatcattac cgggcgaaag gctaaactgg tagaagatcg
    50821 ttgtgccaca ttggggatca ctcacttgta tcaggggcag tcaaacaaac tgatcgcctt
    50881 tagcgaactg ctggaaaaac tggcgattgc cccggaaaat gtggcttatg tcggcgatga
    50941 tctcatcgac tggccggtaa tggaaaaagt gggtttaagc gtcgccgtgg ccgatgctca
    51001 tccactgttg atcccgcgcg ccgattatgt gacgcgcatt gctggcggtc gtggcgcagt
    51061 gcgagaagtt tgcgacttat tactcctggc gcagggcaaa ctggatgaag ccaaagggca
    51121 atcgatatga gtaaagccag acgttgggtt accattgtgc tatcactggc ggttctggtg
    51181 atgatcggca ttaatatggc cgaaaaagac gataccgccc aggtggtcgt caacaacaat
    51241 gatcccacct ataaaagcga gcatacggac acgctcgtct ataacccaga aggggcacta
    51301 agctatcgat tgattgctca acacgttgaa tattattccg atcaggccgt ttcgtggttt
    51361 acgcagccgg tacttaccac gtttgataag gataaaatcc cgacatggtc cgtaaaagca
    51421 gataaagcca agctgaccaa tgaccggatg ctctatttat atggacacgt tgaagtcaac
    51481 gcacttgtgc cggactctca acttcgcaga atcacgacgg ataacgcgca gatcaatctg
    51541 gtgacgcagg atgttacctc tgaagacctc gtcacgttat acggaacaac atttaactcc
    51601 agcggtctga aaatgcgcgg caacttacgc agcaagaacg ccgagctgat tgaaaaggtt
    51661 agaacatcct atgaaattca aaacaaacaa actcagcctt aatcttgtgc ttgccagctc
    51721 acttctggcc gccagcattc cggcatttgc cgtaaccgga gacactgatc agccgatcca
    51781 cattgaatcg gaccagcaat ctcttgatat gcaaggcaac gtggttacct ttaccggtaa
    51841 tgtcatcgtc acccagggca ccatcaaaat taatgccgac aaagtggtcg ttacccgtcc
    51901 gggcggcgaa caaggtaaag aagtgattga cggctacggt aaaccggcaa cgttctacca
    51961 gatgcaggac aacggtaaac ccgttgaagg tcatgcttcc cagatgcact acgaactggc
    52021 aaaagatttt gtcgttctga cgggtaatgc ttatctgcag caggtcgata gcaacattaa
    52081 gggcgataag atcacttacc tggtgaaaga gcagaaaatg caggctttca gcgacaaagg
    52141 caagcgcgta acaaccgttc tggtgccgtc gcagctgcag gacaaaaaca acaaaggcca
    52201 gaccccggca cagaagaagg gtaattaatt cgttatggca acattaactg caaagaacct
    52261 tgcaaaagcc tataaaggcc gtcgcgtggt agaagacgtc agcctgaccg tcaactccgg
    52321 ggaaattgtc ggtctgctgg ggccaaacgg tgccggtaag accaccactt tctatatggt
    52381 tgttggcatt gtgccgcgcg atgcgggcaa catcattatt gatgatgacg atatcagtct
    52441 gctgcctctg catgcacgcg cgcgccgcgg tatcggctat ctgccacagg aagcctccat
    52501 tttccgtcgc ctcagcgttt acgataacct gatggcggta ctgcaaattc gtgacgactt
    52561 gtctgctgaa caacgtgaag accgcgcgaa cgagctgatg gaagagtttc acattgagca
    52621 cctgcgtgac agcatggggc agtcactctc cgggggtgaa cgtcgccgtg tagaaattgc
    52681 ccgcgcactg gctgcgaatc cgaaatttat tctactcgac gaaccgtttg ccggggttga
    52741 cccgatctcg gttatcgaca ttaaacgcat cattgaacac ctgcgcgaca gcggcctggg
    52801 cgtactgatc actgaccaca acgtgcgtga aacactggcg gtttgtgaac gcgcttatat
    52861 cgtcagtcag gggcatttga ttgcccacgg cacgcctaca gaaatcttac aagacgaaca
    52921 cgttaagcgt gtataccttg gggaagactt cagactctga tagggtagaa gtttgcgacg
    52981 ttttagcagg agagtacgat tctgaacatg aagcaaggtt tgcaactcag gcttagccaa
    53041 caactggcga tgacgccaca gcttcaacag gcgattcgtc tgttgcagtt gtcgacgctg
    53101 gaacttcagc aggagctaca gcaggcgctg gagagtaatc cgctgcttga gcaaatcgac
    53161 actcatgaag aaatcgacac cagcgaaacg caagacagtg aaacgctgga caccgccgac
    53221 gcgctcgaac aaaaagagat gccggaagag ctgccgctcg atgccagttg ggacaccatt
    53281 tacaccgctg gtacaccatc cggcaccagc ggtgactaca ttgacgatga gctgccggtc
    53341 tatcagggcg aaacgacgca gaccttgcag gattacctga tgtggcaggt cgagctgaca
    53401 ccgttttccg acactgaccg cgctattgct acctctatcg tcgatgccgt tgatgacacc
    53461 ggttatctga ctgtcccgct ggaagatatt ctcgaaagta tgggcgatga agagatcgac
    53521 atcgacgagg ttgaagccgt ccttaagcgg atccaacggt ttgatccggt cggtgtagcg
    53581 gcaaaagatc tgcgtgactg cctgctaatc caactctccc aattcgataa aaccacgccg
    53641 tggctggaag aggccagact gatcattagc gatcatctcg atctgttagc caatcacgac
    53701 ttccgcactt taatgcgcgt cacgcgtctg aaagaagatg tgctgaaaga agccgtcaat
    53761 ctgatccagt cgctcgatcc gcgccccggg cagtcgatcc agactggcga acctgagtat
    53821 gtcattccag atgtgctggt gcgtaagcat aacggtcact ggacggtaga actcaacagt
    53881 gacagcattc cgcgtctgca aatcaaccag cactacgcct cgatgtgcaa taacgcgcgc
    53941 aacgatggtg acagccagtt tatccgcagc aatctgcagg atgccaaatg gttgatcaag
    54001 agtctggaaa gccgtaacga tacgctactg cgcgtgagtc gctgtatcgt tgaacagcag
    54061 caagccttct ttgagcaagg tgaagaatat atgaaaccga tggtactggc cgatatcgcc
    54121 caggctgtcg aaatgcatga atcgacgata tctcgcgtga ccacgcaaaa atacctgcat
    54181 agtccacgag gcatttttga actgaagtat ttcttttcca gtcacgtcaa taccgagggc
    54241 ggcggcgaag cttcctccac ggcgattcgt gcgctggtga agaaattaat cgcggcggaa
    54301 aacccagcga aaccgttgag cgacagcaag ttaacctctt tgctgtcgga acaaggtatc
    54361 atggtggcac gccgcactgt tgcgaagtac cgagagtctt tatccattcc gccgtcaaac
    54421 cagcgtaaac agctcgtttg acccaaccga taaggaagac actatgcagc tcaacattac
    54481 cggaaataac gtcgagatca ccgaggcact gcgcgaattt gttagagcca aatttgccaa
    54541 acttgagcaa tattttgacc gaatcaacca ggtctatgtt gttctgaaag tggagaaagt
    54601 cacccacacc tcagatgcaa cactgcatgt aaacggtggt gaaattcatg ccagcgcgga
    54661 aggtcaggac atgtacgctg ccattgatgg cttaattgat aagctggcac gccagttgac
    54721 caaacataaa gataaactga aacaacacta attgtccggg caattagcat gtgcatggcg
    54781 gcctgttgtg cggcacaacg ggccatttgt acggttaatg ctccgagcct gttccactgt
    54841 ttgagtgggc aggttcttag gtgaaattat gacaaataat gatacaactc tacagcttag
    54901 cagtgttctt aacagggaat gtacgcgaag ccgcgttcac tgtcagagca aaaaacgcgc
    54961 cctggaaatc atcagcgagc tggcggcgaa acaacttagc ctgccgcctc aggtggtttt
    55021 tgaagctatc ctgacgcggg aaaaaatggg cagtaccggt atcggcaatg gtattgccat
    55081 tccgcatggc aaactggaag aagatactct gcgcgccgtt ggcgttttcg ttcagctcga
    55141 aacgcctata gctttcgatg ccatcgacaa ccaaccggtg gacttacttt ttgccctgct
    55201 ggtgccggca gaccaaacta aaacgcacct gcatactctg tcgctggtgg cgaaacgtct
    55261 ggcggacaaa accatctgcc gccgtttacg cgcagcccag agcgatgaag agctgtatca
    55321 aatcattacg gataccgaag gtactccgga tgaagcgtag ttattcggta atgtctcttt
    55381 tagacgttgt gaggagaaac agtacatggt actgatgatc gtcagcggac gttcaggttc
    55441 aggtaaatct gtcgccctgc gtgcgctgga agatatgggt ttttactgcg tggataacct
    55501 tcccgtagtg ttgttacccg atctggctcg aaccctggcc gatcgcgaga tttctgccgc
    55561 cgtcagcatt gatgttcgta atatgccgga gtcaccagaa atattcgaac aggcgatgag
    55621 taacctgcct gacgctttct caccgcaact cctgttcctg gatgccgacc gtaatacctt
    55681 aattcgtcgt tacagtgaca cgcgccgact gcatccgctt tccagcaaaa acctgtcgct
    55741 ggaaagtgct atcgacaaag aaagcgattt gctggagcct ctgcgttcgc gagcggatct
    55801 gattgtcgat acctcagaaa tgtccgttca cgagctggct gaaatgctgc gtacccgtct
    55861 gctgggtaaa cgtgaacgcg aactgaccat ggtctttgag tctttcggct tcaaacacgg
    55921 tatccctatc gatgcagatt acgtctttga cgtgcgcttc ttgccgaacc cgcactggga
    55981 tccgaaactg cgtccaatga caggtcttga taaacctgtc gccgcgttcc tcgaccgcca
    56041 cacagaagta cacaatttta tctaccagac gcgaagctat cttgagctat ggttacctat
    56101 gctggaaacc aacaaccgta gctacttgac ggtcgccatt ggttgtaccg gcgggaagca
    56161 ccgttcggtg tatattgcag agcaactggc agactacttc cgctcgcgcg gtaaaaacgt
    56221 ccagtcacgc catcgtacgc tggaaaaacg taaaccatga ccgtcaagca aactgttgaa
    56281 atcacaaaca agctgggcat gcatgcccgg cctgcaatga agctgtttga attaatgcag
    56341 ggttttgacg ctgaagtgct gttacgtaat gacgaaggca ccgaggccga agccaacagc
    56401 gttattgcgc tgctgatgtt ggattctgcc aaaggacggc agattgaagt tgaagcgacc
    56461 ggcccacagg aagaggaagc actggccgcc gttatcgccc tctttaactc tggttttgat
    56521 gaagattaat cttcatcact ttgacataca actcccttca aactcccccc gacaataaga
    56581 aaatcacgta ctgaaatcgt tctcaatcaa cgtcatttgt acattttgtg cgcttttcac
    56641 tttcagaaga accttaagaa aaccttaaga ggcattgttt aggttttgtt taagttaatc
    56701 gaccatactg gagatcgtca gaaaatattt ccaggagatg gcatgattcg cttatctgaa
    56761 caaagtcccc tgggcaccgg aagacatcgg aaatgttatg cgcatccgga agatgcccaa
    56821 cgctgtatca agattgtcta ccatcgtggc gatggtggcg ataaagagat ccgccgtgag
    56881 ttaaagtact acgcgtatct tggtcgccgg ttaaaagact ggagtggaat accgcgctat
    56941 cacggtaccg ttgagacgga ttgcggaacc ggttatgtct acgatgtgat tgcagatttt
    57001 gacggcaaac cctccattac gctgaccgaa tttgctgaac aatgccgtta cgaagaagac
    57061 attgcacaac tgcgccagct actgaaacag ctgaagcgtt atttgcagga taaccgtatc
    57121 gtgacgatgt cgctgaagcc gcagaatatc ctctgtcacc acatcagtga gtcagaggtt
    57181 atcccggtcg tctgcgacaa cattggtgaa agcacattga ttccactggc gacctggtcg
    57241 aagtggtgtt gtctgcgtaa gcaagagcgg ttatggaaac gatttatcgc ccaaccggcg
    57301 ctggctattg ccctgcagaa agatctgcaa cctcgtgaga gcaaaacgct ggccctcact
    57361 tcgcgcgaag cttaatccag ctggtgttgt tgcataaacg gctcaccgcc taactgatac
    57421 atctgccgta aaatccacgc ctgacggcta cgcacgtagc ccgatggcga ggagactttg
    57481 aaacgaagtg gattaggtaa tacagctgcc agtaatgcag cttccgaccg ggtaagtttg
    57541 ctcgcgggtt tgtggaaata acgttgtgcc gcagcttcga cgccaaacac accgtcgcca
    57601 aattcggcga tattcaggta aaccgtcagg atacgctttt tgctccagac tgtttctatc
    57661 cccagcgtta atccagcttc cagccctttt cgtacccagc tacgcccatc ccataaaaag
    57721 agatttttgg ccgtctgctg agaaatcgtt gaagcaccgc gaatacggtt ttcattgcgc
    57781 tcgttgtgcg ccagggcttt ctcaatggaa gcgacatcaa agccccggtg ctcaggaaat
    57841 ttctgatctt ctgcggcaat caccgccagt cccatccacg gcgagatttg atccatactg
    57901 acccagtcag aatgtgccac gtaacgaaaa ttgccatgca gccaggcgct gacctgtcgc
    57961 tcgaccatta ccgctgagaa gggaacaggc gcaacactaa acaacgcgat gcccccgccc
    58021 cagaaaacgg cgaggacaac cattaaccgc aaaagaaaac ggcgaacgaa actaaacacc
    58081 gttaagcggc ttttactcat tcagccagaa ccagcacgcg ggaaaccagc ttatcaatgc
    58141 cgctcgccgc ttctgcaatg ttctgcgcca gcatatatgc tggggtggtg acaatcttat
    58201 tgtcttcatc aaccacgata tcatcgacag gacagggcac atgctccgcg cccatctctt
    58261 ccagcacttc tgcggtatcg atatcagtac cgatggtcaa acgcagcggg aaatcgaaaa
    58321 ttttcggcag catcgccggg gcaatacaca taaaaccaag cggttttccg gcctgatgca
    58381 tcgcttgtgc cagcgccttt aattcacggt caacggtgca ttcgctacca agagtggcaa
    58441 aattgcttaa attcttcgcc gcgccaaacc cccccggcac aatcaacgca tccagttcag
    58501 cggcatcggc ctgggccaga ggacggattt caccgcgcgt tattcgtgcc gcttcaatca
    58561 gcacgttgcg cgtttccgtc atcgcttcgc cagttaaatg gttgataaca tcaacctgct
    58621 gcttatcagg tgcaaagcag accgcctgcg caccgctgcg tgaaatagct aacaacgtca
    58681 acaccgcttc atgaatttca gaaccgtcat agacgccgca tccgctcaga attacgccaa
    58741 ttttcttcat tgtgatcatc ctttcgcaat tgactgaaac acattaattt ttttaataaa
    58801 aatggtacgc atcacacatt taactgattc atgtaacaaa tcatttaagt tttgctatct
    58861 taactgcgtg cggcctgaaa aacagtgctg tgcccttgta actcatcata ataatttacg
    58921 gcgcagccaa gatttccctg gtgttggcgc agtattcgcg caccccggtc tagccggggt
    58981 cattttttag tggcttttgc cacccacgct ttcagcactt ctacgtcgtg acgccactct
    59041 tctttcatct cttcaatcca ttcaccgacg ttatcttccc aggcaggaag gtcaggagac
    59101 tgaatttgct gacccagctg ttgcagatgg cgtaacccca ctgaccccgc cgcaccttta
    59161 attttatgtc cttcctcaac aatgcctttt ttatcctgcg ccgtcagatt cgactccagc
    59221 acgctgacat agcccggcat catcttctca aacaccgcta acccgtcggt gatcagcttc
    59281 ggtcctacaa gttcgagata ctgttccagc atgggaatat cgagcaatgc ttctgattta
    59341 ctgttctctt ctgtcgtcac cgtactctcc tcatcatcct gggtatccca gaatttcttg
    59401 atcatcgcgg ttagcgccgg aaccgaaagc ggcttactca gcacatcatc cattccagca
    59461 ttgaggtact cttgtttgtc tttcagcacg ttagcggtta aggccaccag cggcggtaaa
    59521 tcctcgcgcg gataacgctt cgtcagttca cgagagatat ccagcccggt catatctggc
    59581 aactgaatat ccagcaacac caggtcgtat tcgcccggtt taaacatctc cagcgccgcc
    59641 ttgccggtca tggcgacatc aacgctgtta cctaattttt ccagcacaga acgcgcaaca
    59701 atcacgttca gttcaatgtc ttccaccagc agcacattca gcgccggtaa aggcatatcg
    59761 tcttcatcaa acgcatcatc gacctcttct gctaccgacg gtgcgtggat cgtcaacgta
    59821 aaggttgaac ctttgccctg ttcgctggta accgtaatat cgccgcccat atttttcgcc
    59881 agacgacgag aaacggccag accaataccg gtgccggtgg caggtttacc gccatgactg
    59941 tctttcacct ggtaatacat ggcgaaaatt ttatccagct catcctgcgg aatgccgata
    60001 ccagagtctt ccacttcaaa atgcagcata tcgccttcat cgtagcgcac gcgcacggta
    60061 acctggcctt gctgggtgaa tttgacggcg ttactgatga ggttccacag gatctgccgt
    60121 aaacgcgtcc cgtcggtaat gacctgatgc ggtaatggca gcgtcggctc caggttaaag
    60181 cgcaatcctt tttgttgcgc ctgcaatgcg gagagatttt ccagatcggc aaggaagctg
    60241 gtgaaatcaa ccggttgatt atcaagctgg accttgcgcc gttccatctt atccatgtcg
    60301 ataatatcgt taaagatatt ccccagcgtg acggccgaaa catggatggt cttgagatat
    60361 ttttcctgct cggcggtgag ttcggtatcc agcagaatgc ggctcagacc gacgataccg
    60421 ttcagcggtg tacgcaattc gtgactgatg gtggagataa acgtcgtttt gtcgcggctg
    60481 gcccgttcaa gcgcatcctg ataccgctta cgctcggtaa tgtcgcgacc aaagcccatc
    60541 aaaccgtgac gtttacccac gcggtcgtag tacggcactt tacggatttc aaagcaggct
    60601 ttgcgcccgt ccgggtaatc cagccactgt tcataggtca gtgacacatt atgacggaac
    60661 actttttcat cggtttcaat gacttttgcg gcggcttccg gtgagtaaac atcagcaggt
    60721 ttcaggtgaa ccagttgttt ttcgcttttt ccggtcagca gctccatcgc gcggttacag
    60781 ccggaaaact ctttatcttc gttacgataa aaaaccaggt cgggtgaagc atcaaggaag
    60841 gaacgtaaga atgaggattg ctgctcgagc tgaatttgtg tctcttcgcg ctctttgatt
    60901 tcaattttca gttggccgaa ggtttcctgc agttctgctt ccgctttttc acgaacggca
    60961 atttcctgat ttagctgggc aatattatct tttaactgaa cgttgaggct caaatcgcgc
    61021 tcgcgcatct cctccagttt ttgcaccagc cgtgacagac gttgtcgtga ctcctccagt
    61081 tgctcgacga ccaccgatag aaagtagacc gcccacggcg taatcagcaa accaaagaag
    61141 atagaacgaa taacatcaat gctttcgacc tgaccatgca gcaccatggt taccgccatt
    61201 tgtaccacaa tggcaagaac gacgagggcc agcgccagca acattgagaa gcgcaccaga
    61261 cctaacttca tcatcaggtc aacataatac tgcgccagca gacgaatttg cttcattagg
    61321 gaattccttc acgacaacct gccccaataa tacccaattc tgacagttac gttataaatt
    61381 gtgtgagaaa tgcggaattc ccctcaacga cctactccgt tggaggtagc cacggacgtc
    61441 ccagcgccga tccctgtacg ccatgttcat tcagatagcg atccagctcg accattcccg
    61501 tccatcgatt ttcgcaccac agcggagcaa gcagcgtcgg gcgacgggcg ctggcggaaa
    61561 tgcggtggta gatcacttcc ggtggcgtat ggcgaatcat ttctccggca gtgagcgtgt
    61621 aatcctccag ttcaataccg ttcaaacgcc ccgcttccca ggctttcgcc ataatgctgc
    61681 ctttcacaat atgcagcgga tgcagcttta tgccgtccac gccggtttca accactcgct
    61741 ccagcgtttg caaacattca gcctgccctt caccaggcag gccgacaatt aagtgggaac
    61801 acaccttcag cccgcgctga cgtgccagct gggttgtacg ctgataacag gcaaaatcat
    61861 ggccgcggtt gatgcgatgc agtgttttgt cgtgggcggt ttgtagcccc agctccagcc
    61921 acacttcgta accctggtcc ttatattcgc aaagcagatc cagcaccgca tccggcacgc
    61981 agtccgggcg ggtaccaaca cacaaaccga caatattggc ctggctcacc gcctgctgat
    62041 acatagaacg cagcacctga acttccgcaa aggtgctagt atacgcctga aagtaggcca
    62101 gatagcgttt agcgcggtta actaaattcg cctggtgcgc cagttgctcg gcaatggaac
    62161 gatgctgctg cgcttcatcg gcaaacgagg caacattaca gaatgtgcag ccaccacgcc
    62221 cgatggtacc gtcacggtta gggcagctaa aaccgccgtg cagcgtcagc ttatgcacct
    62281 tttgcccata acgacgggtg agatcaccac caaacatatt gactaatttc tgtaactgca
    62341 taatctgata gaccgcgcct tgaaaagagg ccaaagcctg ccatttttag ctcaattcgg
    62401 cgatgacctg gatcaatcgt cccggcctgc ttttatcaac tgcataatca atcaaaatta
    62461 ccgaaatttc atgcataatc acataaatca cttttgctta tcttgtgtca gattttttta
    62521 tctccttatg gattttaggc aaaaacagta gcatgaaacg tcattaccaa ttaaggcagt
    62581 ataaaatgct ggttttctcg tcagttcaag gtaggataag ggttaacacg cctttatgac
    62641 agtcaggaat tgactgtttc tctaacgact tcccttttag ccttaaagat aaaattcatt
    62701 ttaatttcag ttacttaata aagaattttg cgctaaagca catttctgta ccaataagct
    62761 tgccatttga cctgtatcag ctttcccgat aagttggaaa tccgctggaa gctttctgga
    62821 tgagcagcct gctcatcata tttatgcagt aattgagatc ccctcttcac cgtattaacc
    62881 gatgcgaaaa ggacaacaag ggggcgaata cgaggcgcgc gtatgacacg caacccccgt
    62941 cgccacgctc tttctgtgcc cgtgcgcagc ggttcggaag tggggttccc gcagagcctg
    63001 ggggaggttc acgatatgtt gtacgataaa tcccttgaga gggataactg tggtttcggc
    63061 ctgatcgccc acatagaagg cgaacctagc cacaaggtag tgcgtactgc aatacacgca
    63121 ctggcccgca tgcagcaccg tggcgcgatt ctcgccgatg gtaaaaccgg tgacggttgc
    63181 ggcttgctgt tacaaaaacc ggatcgcttt tttcgcatcg ttgcgcagga gcgcggctgg
    63241 cgtttagcaa aaaactacgc tgtcgggatg ctcttcctga ataaagatcc tgaactcgcg
    63301 gctgccgcac gccgcatcgt tgaagaagag ctgcaacgcg aaaccttgtc gattgtgggc
    63361 tggcgtgatg tccccactaa cgaaggcgtg ctgggtgaaa tcgccctctc ctctctgccc
    63421 cgcattgagc aaattttcgt taacgctccg gcaggctggc gtccgcggga tatggagcgc
    63481 cgtctgttta tcgcccgccg ccgcattgaa aagcgtctcg aagccgacaa agacttctac
    63541 gtctgtagcc tgtcgaatct ggtgaacatc tataaaggtc tgtgtatgcc ggcggatctg
    63601 ccgcgctttt atctggatct tgcggacctg cgtctggaat cggccatctg cctgttccac
    63661 cagcgcttct ccactaacac cgtaccgcgc tggccgctgg cgcagccgtt ccgctacctg
    63721 gcgcataacg gtgaaatcaa caccatcacc ggtaaccgcc aatgggcgcg tgcgcgtacc
    63781 tataaattcc agacgccgct tatccctgat ctgcacgacg ccgcaccgtt cgtcaacgaa
    63841 accggctctg actccagttc gatggataac atgctggaac tgctgctggc aggcgggatg
    63901 gatatcatcc gcgccatgcg tctgttagtg ccacccgcct ggcagaacaa cccggatatg
    63961 gacccggaac tgcgtgcctt ctttgacttt aactccatgc atatggagcc gtgggatggc
    64021 ccggcgggca tcgtgatgtc cgacggtcgt tttgccgcct gtaacctcga ccgtaacggt
    64081 ctgcgtccgg cgcgctacgt catcaccaaa gataagctca tcacctgcgc ctctgaagtc
    64141 ggtatctggg attaccagcc tgacgaagtg gtcgaaaaag gccgcgtcgg gccaggcgaa
    64201 ctgatggtta tcgacacccg cagtgggcgt attctgcact cggcagaaac cgatgacgat
    64261 ctgaaaagcc gccatccata taaagagtgg atggagaaaa acgtccgccg actggtaccg
    64321 tttgaagatc tgcccgatga agaagtgggt agccgcgaat tggacgacga cacgcttgcc
    64381 agctaccaga aacagtttaa ctacagtgcg gaagagctgg actccgtaat tcgcgtactg
    64441 ggcgaaaacg gtcaggaagc ggtcggttcg atgggcgatg ataccccatt cgccgtgctc
    64501 tccagtcagc cgcgcattat ttacgactac ttccgccagc agtttgccca ggtgactaac
    64561 ccgccaatcg acccgctgcg tgaagcgcat gttatgtcgc tcgccaccag tatcggtcgt
    64621 gaaatgaacg tcttttgcga agcagagggc caggcgcacc gtttaagctt taaatcgccg
    64681 attctgctct actccgattt caaacagctc acgacgatga aagaggagca ctaccgcgca
    64741 gatacgctgg atatcacctt tgacgtcact aaaaccacgc tcgaagcgac agtcaaagag
    64801 ctgtgcgaca aagccgaaaa aatggtacgt agcggcaccg tgctgctggt gctctccgac
    64861 cggaatatcg ctaaagatcg cctgccggtt ccagccccga tggcggttgg cgcgatccag
    64921 acccgtctgg tcgatcaaag cctgcgttgc gatgccaaca tcatcgtcga aaccgccagc
    64981 gcccgcgatc cgcaccactt cgccgtgttg ctgggcttcg gcgcgacggc tatttatcca
    65041 taccttgcct atgaaacgct gggccgcctg gttgacaccc atgcgattgc caaagattat
    65101 cgtaccgtga tgctcaacta ccgtaacggc atcaacaaag gcttgtacaa aatcatgtcc
    65161 aaaatgggca tctccaccat cgcctcttac cgctgctcga aactgtttga agcggtcggt
    65221 ctgcacgatg atgtagtggg cctgtgcttc cagggggcgg tcagccgcat tggtggggcg
    65281 agctttgaag acttccagca ggatctgctg aacctgtcga aacgtgcctg gctggcgcgt
    65341 aagcccatca gccagggcgg cctgctgaaa tacgtccacg gcggcgaata ccacgcttac
    65401 aacccggacg tggtgcgcac gctgcaacag gcggtacaaa gcggcgagta cagcgactat
    65461 caggaatacg cgaagctggt taatgagcgt ccggcaacca cgctgcgcga tctgctggca
    65521 attacgccgg gtgaaaacgc ggtcaacatt gctgatgttg aaccggcaag cgaactgttt
    65581 aaacgctttg ataccgccgc gatgtctatc ggcgcgttaa gcccggaagc ccacgaggcg
    65641 ctggcggaag cgatgaatag catcggcggt aactcgaact ccggtgaagg cggcgaagac
    65701 ccggcgcgct atggcactaa caaagtgtcg cgcatcaagc aggtggcttc tggtcgcttc
    65761 ggcgttactc cggcgtatct ggtcaatgcc gacgtcattc agattaaagt cgcccagggc
    65821 gcgaagccgg gcgaaggcgg tcagttgccg ggcgacaaag tcactcctta catcgccaaa
    65881 ctgcgctatt cggtgcccgg agtgacgctg atctccccgc cgccgcacca cgatatctac
    65941 tctatcgagg acttagcgca gctcattttc gacctcaagc aggttaaccc gaaagcgatg
    66001 atctccgtga agctggtttc cgaaccgggc gtaggcacca tcgcgactgg cgtggcaaaa
    66061 gcttatgccg atttaatcac catcgcaggc tatgacggtg gcaccggcgc aagcccgctg
    66121 tcgtcagtga aatacgcagg ttgcccgtgg gagctggggc ttgttgaaac ccagcaggcg
    66181 ctggttgcca atggtctgcg tcataagatc cgtttgcagg tcgatggcgg cctgaaaacc
    66241 ggtgttgata tcatcaaagc ggcgattctc ggcgcagaaa gcttcggctt cggcactggc
    66301 ccaatggtcg cgctcggctg taaatatctg cgtatttgcc acctgaacaa ctgcgcaacg
    66361 ggtgtggcaa ctcaggatga caaactgcgt aagaatcact atcacggcct gccgttcaag
    66421 gtgacgaatt actttgagtt tatcgcccgt gaaacccgcg agctgatggc acagctgggg
    66481 gtaacgcggc tggtggatct gattggtcgc accgacctgc tgaaagagct ggacggtttc
    66541 accgccaaac agcagaaact ggcgctgtcg aagctgctgg agaccgccga accgcatcca
    66601 ggtaaggcac tctactgcac cgaaaacaac ccgccgtttg ataacggcct gctgaacgcg
    66661 cagttgctgc aacaggcgaa accgtttgtc gatgaacgcc agagcaaaac cttctggttc
    66721 gatatccgca ataccgatcg ctccgtgggc gcgtcgcttt caggctatat cgcccagacg
    66781 cacggcgatc aggggctggc agccgatcct atcaaagcgt acttcaacgg caccgcaggc
    66841 cagagtttcg gtgtatggaa cgcgggcggc gtggaactgt acctgaccgg ggatgccaac
    66901 gactatgtcg gtaaaggcat ggcgggcggc ttaatcgcca ttcgtcctcc ggttggttcc
    66961 gccttccgca gccatgaagc aagtattatc ggcaacacct gcctgtatgg cgcgaccggt
    67021 ggtcgtctgt atgccgcagg ccgcgcgggt gaacgtttcg gcgtgcggaa ctccggtgct
    67081 atcaccgtgg tagaaggcat tggcgacaac ggctgtgaat atatgacggg cggtattgtc
    67141 tgtattctgg gtaaaaccgg cgttaacttc ggtgcgggca tgaccggcgg cttcgcttac
    67201 gttctcgatg aaagcggtga tttccgcaaa cgcgttaacc cggaactggt cgaggtctta
    67261 aacgttgacg agctggcgat ccatgaagag catctgcgcg gtcttatcac cgagcatgtt
    67321 cagcatactg gctctcagcg cggtgaagag attctggcga actggtcaac cttcgccact
    67381 aaatttgcgc tggttaaacc gaagtccagt gatgtaaaag cactgctggg tcaccgtagt
    67441 cgtagcgcag cagagttgcg cgtgcaggcg cagtaagggg tagcaacaat gagtcagaat
    67501 gtttatcaat ttatcgacct gcagcgcgtt gatccgccta agaaaccgct gaagatccgc
    67561 aaaattgagt ttgttgaaat ttacgagcag ttttccgaag gccaggccaa agcgcaggct
    67621 gaccgctgcc tgtcgtgcgg caacccatac tgcgagtgga aatgtccggt acacaactac
    67681 atcccgaact ggctgaagct cgccaacgag gggcgtattt ttgaagcggc ggaactgtcg
    67741 caccagacca acaccctgcc ggaagtttgc ggacgagtct gcccgcaaga ccgtctgtgc
    67801 gaaggttcct gcactctgaa cgatgagttt ggcgcggtga ccatcggcaa cattgagcgt
    67861 tatatcaatg ataaagcgtt cgaaatgggc tggcgtccgg atatgtccgg cgtgaagcaa
    67921 accggtaaaa aagtggcgat tatcggcgca ggcccggcag gtctggcgtg tgcggatgtc
    67981 ctgacgcgta acggcgtaaa agccgttgtc ttcgaccgtc atccagaaat tggcgggctg
    68041 ctgaccttcg gtattccggc cttcaagctg gaaaaagagg taatgacgcg tcgccgtgaa
    68101 atcttcaccg gcatgggtat tgaattcaaa ctcaataccg aagtgggccg cgacgtgcag
    68161 ctggacgatc tgctgagtga ttacgatgcc gtcttccttg gcgtcgggac ttatcagtca
    68221 atgcgcggcg gtctggaaaa caaagacgcc gatggcgtgt acgcagcgct gccgttcctt
    68281 atcgccaaca ccaaacagtt aatgggcttt ggtgaaaccc gcgacgaacc gttcgtcagc
    68341 atggaaggca aacgcgtggt ggtccttggc ggtggcgaca ctgcgatgga ctgcgtgcgt
    68401 acgtccgtgc gccagggagc gaagcacgtt acctgtgcct atcgtcgtga tgaagagaac
    68461 atgccgggtt cccgccgcga agtgaaaaac gcgcgggaag aaggcgtaga gttcaaattc
    68521 aacgtccagc cactggggat tgaagtgaac ggtaacggca aagtcagcgg cgtaaaaatg
    68581 gtacgtaccg aaatgggcga accggatgcc aaaggccgtc gccgcgcgga gatcgttgca
    68641 ggttccgaac atatcgttcc ggcagatgca gtgatcatgg cgtttggttt ccgtccacac
    68701 aacatggaat ggctggcaaa acacagcgtc gagctggatt cacaaggccg catcatcgcc
    68761 ccggaaggca gcgacaacgc cttccagacc agcaacccga aaatctttgc tggcggcgat
    68821 atcgtccgtg gttccgatct ggtggtaacc gctattgccg aaggtcgtaa ggcggcagac
    68881 ggtattatga actggctgga agtttaagct ctgtcccagc gccgcctcct cagaaaagag
    68941 tcggcgctga aattctcctt tctttctgga aagcgcctcg cataatctga agaaaaccaa
    69001 tgtaatacgc tcatctggat tgttaccggt agatgcccgg cgtatagctg attccattat
    69061 tcaacgcagc gccatataaa gggacagata atggaatcgc tctcagagag aacctcaaca
    69121 ggctaccagc aaatccacga cggtattatt cacctggtcg atagcgcccg gacggaaacg
    69181 gtacgtagcg ttaacgcgct gatgaccgcg acatactggg aaattggccg acgaattgtc
    69241 gaatttgaac aaggtggcga ggcaagggct gcgtatggtg cgcagctaat caagcgacta
    69301 tcaaaggatt taagtctaag gtataagcgt gggttctctg caaaaaactt acgccaaatg
    69361 aggctttttt acctcttttt tcaacatgtt gaaattcgcc agacagtgtc tggcgaatta
    69421 acaccattgg gaattccgca gacaccatct gcagaattcc catcggcaaa gatttggcag
    69481 acgctgtctg ccaaattgag aggctgtttg atatttccac agcttttgta ttgcatctcc
    69541 tattttcggt ttgtatattc atgcaaataa acgaaggaaa gttgccatga tttacattga
    69601 acagcttgaa ttaatccata aaagtggtga tgtgttgtat ccagtaaaaa taaccagaaa
    69661 atcatcagga aaaactgcct tccatcttgt tccattcggc ttaaataaga ctcacgacct
    69721 tctagaggtg gaagatgctt cagaagcaat tcggctggtg attgatgaac gtcattccat
    69781 tcgctgcagt acacttacag caactattac gaataaaaag ggcaagcgaa ttaagcggac
    69841 aggtatatac agtatcaag
//
LOCUS       DAZPEB010000005        58844 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZPEB010000005 DAZPEB010000000
VERSION     DAZPEB010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58844
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.005"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            102..2624
                     /gene="mrcB"
                     /locus_tag="ACHURJ_000303"
     CDS             102..2624
                     /gene="mrcB"
                     /locus_tag="ACHURJ_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFR1279391.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRAHLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGLVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTDTWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWAGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            2844..5033
                     /gene="fhuA"
                     /locus_tag="ACHURJ_000304"
     CDS             2844..5033
                     /gene="fhuA"
                     /locus_tag="ACHURJ_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFR1279392.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMHQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTTEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIAGDGHTLNRGTVVDNERLQNFSVDTQLESKFATGDVEHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGSAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRISYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGDYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSAAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            5084..5881
                     /gene="fhuC"
                     /locus_tag="ACHURJ_000305"
     CDS             5084..5881
                     /gene="fhuC"
                     /locus_tag="ACHURJ_000305"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFR1279393.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSSKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGDTLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            5881..6771
                     /gene="fhuD"
                     /locus_tag="ACHURJ_000306"
     CDS             5881..6771
                     /gene="fhuD"
                     /locus_tag="ACHURJ_000306"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFR1279394.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSAEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            6768..8750
                     /gene="fhuB"
                     /locus_tag="ACHURJ_000307"
     CDS             6768..8750
                     /gene="fhuB"
                     /locus_tag="ACHURJ_000307"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFR1279395.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWMWASGALLDDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLYRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATSTMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(8785..10065)
                     /gene="hemL"
                     /locus_tag="ACHURJ_000308"
     CDS             complement(8785..10065)
                     /gene="hemL"
                     /locus_tag="ACHURJ_000308"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFR1279396.1"
                     /translation="MSKSENLYSAARELLPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPQPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGTPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            10231..10278
                     /gene="yadX"
                     /locus_tag="ACHURJ_000309"
     CDS             10231..10278
                     /gene="yadX"
                     /locus_tag="ACHURJ_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFR1279397.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            10290..11711
                     /gene="clcA"
                     /locus_tag="ACHURJ_000310"
     CDS             10290..11711
                     /gene="clcA"
                     /locus_tag="ACHURJ_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFR1279398.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMEALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVVVELFPQYHLESGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSEILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            11735..11800
                     /gene="yadW"
                     /locus_tag="ACHURJ_000311"
     CDS             11735..11800
                     /gene="yadW"
                     /locus_tag="ACHURJ_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFR1279399.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            11793..12137
                     /gene="erpA"
                     /locus_tag="ACHURJ_000312"
     CDS             11793..12137
                     /gene="erpA"
                     /locus_tag="ACHURJ_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFR1279400.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(12184..12807)
                     /locus_tag="ACHURJ_000313"
     CDS             complement(12184..12807)
                     /locus_tag="ACHURJ_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFR1279401.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(12845..13644)
                     /gene="btuF"
                     /locus_tag="ACHURJ_000314"
                     /pseudo
     CDS             complement(12845..13644)
                     /gene="btuF"
                     /locus_tag="ACHURJ_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
     gene            complement(13637..14335)
                     /gene="mtnN"
                     /locus_tag="ACHURJ_000315"
     CDS             complement(13637..14335)
                     /gene="mtnN"
                     /locus_tag="ACHURJ_000315"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFR1279402.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHGKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            14419..15936
                     /gene="dgt"
                     /locus_tag="ACHURJ_000316"
     CDS             14419..15936
                     /gene="dgt"
                     /locus_tag="ACHURJ_000316"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFR1279403.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRMKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            16066..17490
                     /gene="degP"
                     /locus_tag="ACHURJ_000317"
     CDS             16066..17490
                     /gene="degP"
                     /locus_tag="ACHURJ_000317"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFR1279404.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            17645..18802
                     /gene="cdaR"
                     /locus_tag="ACHURJ_000318"
     CDS             17645..18802
                     /gene="cdaR"
                     /locus_tag="ACHURJ_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HFR1279405.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGRLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIYRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(18891..19277)
                     /locus_tag="ACHURJ_000319"
     CDS             complement(18891..19277)
                     /locus_tag="ACHURJ_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFR1279406.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(19439..20251)
                     /gene="yaeI"
                     /locus_tag="ACHURJ_000320"
     CDS             complement(19439..20251)
                     /gene="yaeI"
                     /locus_tag="ACHURJ_000320"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HFR1279407.1"
                     /translation="MISRRRLLQATAATIATSSGFGYMHYWEPGWFELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMPLNFSAFS
                     DVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSVGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASEANLPRLMLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGSLYGLRLNCRPEVTMLELV"
     gene            complement(20306..21130)
                     /gene="dapD"
                     /locus_tag="ACHURJ_000321"
     CDS             complement(20306..21130)
                     /gene="dapD"
                     /locus_tag="ACHURJ_000321"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFR1279408.1"
                     /translation="MQQLQNIIETAFERRAEITPTNADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGHVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(21161..23833)
                     /gene="glnD"
                     /locus_tag="ACHURJ_000322"
     CDS             complement(21161..23833)
                     /gene="glnD"
                     /locus_tag="ACHURJ_000322"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFR1279409.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(23895..24689)
                     /gene="map"
                     /locus_tag="ACHURJ_000323"
     CDS             complement(23895..24689)
                     /gene="map"
                     /locus_tag="ACHURJ_000323"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFR1279410.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            25057..25782
                     /gene="rpsB"
                     /locus_tag="ACHURJ_000324"
     CDS             25057..25782
                     /gene="rpsB"
                     /locus_tag="ACHURJ_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFR1279411.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            26040..26891
                     /gene="tsf"
                     /locus_tag="ACHURJ_000325"
     CDS             26040..26891
                     /gene="tsf"
                     /locus_tag="ACHURJ_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFR1279412.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            27038..27763
                     /gene="pyrH"
                     /locus_tag="ACHURJ_000326"
     CDS             27038..27763
                     /gene="pyrH"
                     /locus_tag="ACHURJ_000326"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFR1279413.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            28055..28612
                     /gene="frr"
                     /locus_tag="ACHURJ_000327"
     CDS             28055..28612
                     /gene="frr"
                     /locus_tag="ACHURJ_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFR1279414.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            28704..29900
                     /gene="ispC"
                     /locus_tag="ACHURJ_000328"
     CDS             28704..29900
                     /gene="ispC"
                     /locus_tag="ACHURJ_000328"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFR1279415.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            30088..30846
                     /gene="ispU"
                     /locus_tag="ACHURJ_000329"
                     /gene_synonym="uppS"
     CDS             30088..30846
                     /gene="ispU"
                     /locus_tag="ACHURJ_000329"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFR1279416.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQECIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFIDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            30859..31716
                     /gene="cdsA"
                     /locus_tag="ACHURJ_000330"
     CDS             30859..31716
                     /gene="cdsA"
                     /locus_tag="ACHURJ_000330"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFR1279417.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVSLLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            31728..33080
                     /gene="rseP"
                     /locus_tag="ACHURJ_000331"
     CDS             31728..33080
                     /gene="rseP"
                     /locus_tag="ACHURJ_000331"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFR1279418.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GLQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSLLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGAHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            33110..35542
                     /gene="bamA"
                     /locus_tag="ACHURJ_000332"
     CDS             33110..35542
                     /gene="bamA"
                     /locus_tag="ACHURJ_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFR1279419.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            35664..36149
                     /gene="skp"
                     /locus_tag="ACHURJ_000333"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             35664..36149
                     /gene="skp"
                     /locus_tag="ACHURJ_000333"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFR1279420.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            36153..37178
                     /gene="lpxD"
                     /locus_tag="ACHURJ_000334"
     CDS             36153..37178
                     /gene="lpxD"
                     /locus_tag="ACHURJ_000334"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFR1279421.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            37283..37738
                     /gene="fabZ"
                     /locus_tag="ACHURJ_000335"
     CDS             37283..37738
                     /gene="fabZ"
                     /locus_tag="ACHURJ_000335"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFR1279422.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            37742..38530
                     /gene="lpxA"
                     /locus_tag="ACHURJ_000336"
     CDS             37742..38530
                     /gene="lpxA"
                     /locus_tag="ACHURJ_000336"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFR1279423.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVGIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFSRSTRGLIR"
     gene            38530..39678
                     /gene="lpxB"
                     /locus_tag="ACHURJ_000337"
     CDS             38530..39678
                     /gene="lpxB"
                     /locus_tag="ACHURJ_000337"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFR1279424.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDFSVHLLDGMGREAMVASDAALLASG
                     TAALECMLVKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKISHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            39675..40271
                     /gene="rnhB"
                     /locus_tag="ACHURJ_000338"
     CDS             39675..40271
                     /gene="rnhB"
                     /locus_tag="ACHURJ_000338"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFR1279425.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALCEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMVVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            40308..43790
                     /gene="dnaE"
                     /locus_tag="ACHURJ_000339"
     CDS             40308..43790
                     /gene="dnaE"
                     /locus_tag="ACHURJ_000339"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFR1279426.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGVNAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYASGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            43803..44762
                     /gene="accA"
                     /locus_tag="ACHURJ_000340"
     CDS             43803..44762
                     /gene="accA"
                     /locus_tag="ACHURJ_000340"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFR1279427.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSSYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            44861..46970
                     /gene="ldcC"
                     /locus_tag="ACHURJ_000341"
                     /pseudo
     CDS             44861..46970
                     /gene="ldcC"
                     /locus_tag="ACHURJ_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
     gene            47027..47416
                     /locus_tag="ACHURJ_000342"
     CDS             47027..47416
                     /locus_tag="ACHURJ_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR1279428.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            47481..48779
                     /gene="tilS"
                     /locus_tag="ACHURJ_000343"
     CDS             47481..48779
                     /gene="tilS"
                     /locus_tag="ACHURJ_000343"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFR1279429.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELAQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSREALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKSAGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(48828..49088)
                     /gene="rof"
                     /locus_tag="ACHURJ_000344"
     CDS             complement(48828..49088)
                     /gene="rof"
                     /locus_tag="ACHURJ_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFR1279430.1"
                     /translation="MLMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(49075..49275)
                     /locus_tag="ACHURJ_000345"
     CDS             complement(49075..49275)
                     /locus_tag="ACHURJ_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFR1279431.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            49441..49986
                     /locus_tag="ACHURJ_000346"
     CDS             49441..49986
                     /locus_tag="ACHURJ_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFR1279432.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            49983..50405
                     /gene="arfB"
                     /locus_tag="ACHURJ_000347"
     CDS             49983..50405
                     /gene="arfB"
                     /locus_tag="ACHURJ_000347"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFR1279433.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSAKAMRGKVRSGRE"
     gene            50419..51129
                     /gene="nlpE"
                     /locus_tag="ACHURJ_000348"
                     /gene_synonym="cutF"
     CDS             50419..51129
                     /gene="nlpE"
                     /locus_tag="ACHURJ_000348"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308221.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFR1279434.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERGYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(51329..52149)
                     /locus_tag="ACHURJ_000349"
                     /pseudo
     CDS             complement(51329..52149)
                     /locus_tag="ACHURJ_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
     gene            complement(52202..53920)
                     /gene="proS"
                     /locus_tag="ACHURJ_000350"
     CDS             complement(52202..53920)
                     /gene="proS"
                     /locus_tag="ACHURJ_000350"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFR1279435.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(54030..54737)
                     /gene="tsaA"
                     /locus_tag="ACHURJ_000351"
     CDS             complement(54030..54737)
                     /gene="tsaA"
                     /locus_tag="ACHURJ_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFR1279436.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYHKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(54734..55138)
                     /gene="rcsF"
                     /locus_tag="ACHURJ_000352"
     CDS             complement(54734..55138)
                     /gene="rcsF"
                     /locus_tag="ACHURJ_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFR1279437.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(55256..56071)
                     /gene="metQ"
                     /locus_tag="ACHURJ_000353"
     CDS             complement(55256..56071)
                     /gene="metQ"
                     /locus_tag="ACHURJ_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFR1279438.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(56111..56764)
                     /locus_tag="ACHURJ_000354"
     CDS             complement(56111..56764)
                     /locus_tag="ACHURJ_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFR1279439.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(56757..57788)
                     /gene="metN"
                     /locus_tag="ACHURJ_000355"
     CDS             complement(56757..57788)
                     /gene="metN"
                     /locus_tag="ACHURJ_000355"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFR1279440.1"
                     /translation="MIKLSNITKVFHQGTRIIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            57976..58548
                     /gene="gmhB"
                     /locus_tag="ACHURJ_000356"
     CDS             57976..58548
                     /gene="gmhB"
                     /locus_tag="ACHURJ_000356"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFR1279441.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFVLVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAAAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPA"
BASE COUNT        14046 a        14490 c        16113 g        14195 t
ORIGIN      
        1 ggggaccgat cgaacacaaa ttttgagaga tatcttcttc tgtcttgtaa cagaagaaca
       61 gaaaatcggg cttttgcgcc tgaatattgc ggagaaaaag catggccggg aatgaccgcg
      121 agccaattgg acgcaaaggg aaaccgacgc gtccggtcaa acaaaaggta agccgtcgtc
      181 gttacgaaga tgacgatgat tacgacgatt atgatgacta tgaggatgaa gaaccgatgc
      241 cgcgcaaagg taagggcaaa ggcaaagggc gtaagcctcg tggcaaacgc ggctggctat
      301 ggctactgct aaaactggct atcgtttttg ccgtgctgat cgccatttac ggcgtttatc
      361 tcgatcaaaa aattcgtagc cgtattgatg gcaaggtctg gcaactgcct gcagcagttt
      421 atggccgaat ggtcaatctt gagccagata tgaccatcag caagaacgag atggtgaagc
      481 tgctggaggc gactcagtat cgtcaggtgt cgaaaatgac ccgtcctggc gaatttaccg
      541 tgcaggccaa cagcattgag atgatccgcc gtccgtttga tttcccggac agtaaagaag
      601 ggcaggtgcg tgcgcatctg acctttgatg gtgatcatct ggcgaccatc gtcaatatgg
      661 agaacaaccg tcagttcggc tttttccgtc ttgatccgcg tctgatcacc atgatctcct
      721 cgccaaacgg tgagcaacgt ctgtttgtgc cgcgcagtgg tttccctgat ttgctggtgg
      781 atactttgct ggcgacagaa gaccgccatt tctacgagca tgatgggatc agcctctact
      841 caattgggcg tgcggtgctg gcaaacctga ccgcaggtcg cacggtgcag ggtgcgagta
      901 cgctgacgca acagctggtg aaaaacctgt tcctctccag cgagcgctct tactggcgta
      961 aagcgaacga agcctacatg gcgctgatca tggacgcgcg ttacagcaaa gaccgtattc
     1021 ttgagctgta tatgaacgag gtgtatctcg gtcagagcgg cgacaacgaa atccgcggct
     1081 tcccgctggc aagcttgtat tactttggtc gcccggtaga agagctaagc ctcgaccagc
     1141 aggcgctgtt agtcggtatg gtgaaagggg cgtccatcta caacccgtgg cgtaacccga
     1201 aactggcgct ggagcgacgt aatctggtgc tgcgtctgct gcaacagcaa cagattattg
     1261 atcaagaact ctatgacatg ttgagtgccc gtccgctggg ggttcagccg cgcggtgggg
     1321 tgatctctcc tcagccagcc tttatgcaac tggtgcgtca ggagctgcag gcaaaactgg
     1381 gcgataaggt aaaagatctc tccggcgtga agatcttcac tacctttgac tcggtggccc
     1441 aggacgcggc agaaaaagcc gccgtggaag gcattccggc actgaagaaa cagcgtaagt
     1501 tgagcgatct tgaaactgcg attgtggtcg tcgaccgctt tagtggtgaa gttcgtgcga
     1561 tggtgggagg ttctgagccg cagtttgcgg gctacaaccg tgcgatgcag gcgcgtcgtt
     1621 cgattggttc ccttgcaaaa ccagcgactt atctgacggc tttaagccag ccgaaaatct
     1681 atcgcctgaa cacgtggatt gccgatgcgc caatcgcgct gcgtcagccg aatggcctgg
     1741 tctggtcacc gcagaacgat gaccgtcgtt atagcgaaag cggcagagtg atgctggtgg
     1801 atgcgttgac ccgttcgatg aacgtgccga cggtaaatct ggggatggcg ctggggctgc
     1861 ctgcagttac ggatacctgg atcaaactgg gcgtaccgaa agatcagctg catccggttc
     1921 cggcgatgct gctgggggcg ttgaacttaa cgccaatcga agtggcgcag gcgttccaga
     1981 ccatcgccag cggtggtaac cgtgcaccgc tttctgcact gcgttcggta atcgcggaag
     2041 atggcaaggt gctgtatcag agcttcccgc aggcagaacg cgctgttccg gcgcaggcgg
     2101 cgtatctgac actatggacc atgcagcagg tggtacaacg tggtacgggt cgtcagcttg
     2161 gggcgaaata cccgaacctg catctggcag ggaaaacagg gactaccaac aataacgtag
     2221 atacttggtt tgcaggcatt gacggcagca cggtgaccat cacctgggca ggccgtgata
     2281 acaaccagcc gaccaaactg tatggagcca gcggggcaat gtcgatttat cagcgttatc
     2341 tggctaacca gacgccaacg ccgctgaatc ttgttccgcc agaagatatt gcagatatgg
     2401 gcgtggacta cgacggcaac tttgtttgca gcggtggcat gcgtgtcttg ccggtctgga
     2461 ccagcgatcc gcaatcgctg tgccagcaga gcgagatgca gcagcagccg tcaggcaatc
     2521 cgtttgatca gtcttctcag ccgcaacagc aacctgctca gcaagagcag aaagacagcg
     2581 acggtgtagc cggttggatc aaggatatgt ttggtagtaa ttaacatcta agcgtgaaat
     2641 accggatggc gagttgccat ccggtaaaat aacatcccat ctaagatatt aaccctttct
     2701 tttcatctgg ttgtttatta acccttcagg aacgctcaga ttgcgtaccg cttgcgaacc
     2761 cgccagcgtt tcgaatatta tcttatcttt ataataatca ttctcgttta cgttatcatt
     2821 cactttacat cagagatata ccaatggcgc gttccaaaac tgctcagcca aaacactcac
     2881 tgcgtaaaat cgcagttgta gtagccacag cggttagcgg catgtctgtt tatgcacagg
     2941 cagcggttga accgaaagaa gacactatca ccgttaccgc tgcacctgcg ccgcaagaaa
     3001 gcgcatgggg gcctgctgca actattgcgg cgaagcactc tgctactgcg actaaaacgg
     3061 atacaccaat tgaaaaaacg ccgcagtcta tttcggttgt gacaaatgaa gagatgcaga
     3121 tgcatcaatt tcagtctgta aaagaagcat taggttatac acctggcgtt acggttagca
     3181 gtcgtggtgc ttctaataca tatgattttg ttatcattcg tggtttctca tctgttggtc
     3241 tgaatcaaaa taattacctt gatggactaa aacttcaggg caacttttat aacgatgctg
     3301 tgattgatcc ttacatgctc gagagggttg aactgatgcg tgggccgacg tctgttctct
     3361 atggaaaaag taatcctggt gggattattt ctatggtgag taagcgtccg accactgaac
     3421 ccctgaaaga aattcagttt aaaatgggga cggataatct gtttcagacc ggattcgatt
     3481 tcagtgatgc actggatgat aacggtgagt tctcttaccg tttgacgggc ctcgctcgct
     3541 caacaaacga acagcagaaa aactctgaat ctcagcgtta taccattgct ccatcattct
     3601 catggcgtcc agacgacaaa accaatttta ctttcctgtc ctatttccag aatgaaccag
     3661 aaacgggtta ttacggttgg ttgccgaaag aggggaccgt tgagccattg cctaatggta
     3721 agcgtctacc gactgacttc aatgaaggtg catcgaataa tacatactcc cgtaaccaga
     3781 aaatggtggg atatagtttt gaacatggtt ttaatgacac cttcaccgtg cgtcagaatc
     3841 tgcgtttcag tgaaatgaaa acctcacaga aaagtgttta tggcacaggg attgctggtg
     3901 atggtcatac cctaaaccgc gggacagtgg tggataatga gcgtctacaa aactttagcg
     3961 ttgataccca acttgaaagt aagtttgcta caggtgacgt cgagcatact ttgctgacag
     4021 gggtagactt catgcggatg cgtaatgata tcaatgccag ctttggatcc gcaccatcca
     4081 tcgatcttta taacaaatat caccctgaat actttgcatt tggtagcgca gagccatacc
     4141 agatgaatga aagcaaacaa acaggtattt atgttcagga tcaggcggaa tggaataaat
     4201 gggtattcac tctgggggga cgctacgatt ggtctaagca agcgaccact gttcgtgaaa
     4261 actcttatac gccgactgaa ggttatattg agcgcaatga tcatcagttc acctggcgcg
     4321 gtggtgtaaa ttacttattc gataatggta tttcacctta ctttagctat agccagtcct
     4381 ttgaaccgag tgctttcgat ctgtggagca acccacgcat ttcctataag ccatcgaaag
     4441 gtgaacagta tgaagctggc gtaaaatatg ttccgaatga tatgccggtc gttgttacgg
     4501 gcgcagtcta tcaattgacg aaaacaaata acctgacagc agacccaaca aacccattag
     4561 cgcaagtccc agcaggtgag attcgcgctc gtggtgtgga acttgaagca aaagcggcgt
     4621 taaatgccaa tattaacttg acggcttctt atacctacac cgatgcggaa tacaccaaag
     4681 acaccaatct caaaggtaaa actccagaac aagtaccgga gcatatggca tctctctggg
     4741 gggattatac cttcaatgaa gggccgcttt ctggtttaac attgggaaca ggtggtcgtt
     4801 ttattggttc cagctatggt gatccggcaa acacttttaa agtgggtagc gcagctgtaa
     4861 tggatgctgt tgtaaaatat gatctggcac gctttggtat ggcgggatcc agccttgctg
     4921 tgaacgtcaa caatttgctc gatcgtgagt atgttgccag ttgcttccag acttatggct
     4981 gcttctgggg cgcagaacgt caggtcgttg caaccgcaac cttccgtttc taatttctct
     5041 tttggggcac ggatttccgt gcccatttca caagttggct gttatgcagg aatacacgaa
     5101 tcattccgat accacttttg cactgcgtaa tatctccttt cgtgtgcccg ggcgcacgct
     5161 tttgcatccg ctgtcgttaa cctttcctgc cgggaaagtg accggtctga ttggtcacaa
     5221 cggttctagt aaatccactc tgctcaaaat gcttggccgt catcagcctc cgtcagaagg
     5281 ggagattctt cttgatgccc agccgctgga aagctggagc agcaaagcgt ttgcccgcaa
     5341 agtggcttat ttgccgcagc agcttcctcc ggcagaaggg atgaccgtgc gtgaactggt
     5401 ggcgattggt cgttacccgt ggcatggcgc gctggggcgc tttggggcgg cagatcgcga
     5461 aaaagtcgag gaagctatct cgctggttgg cttaaaaccg ctggcgcatc ggctggttga
     5521 tagtctctct ggcggcgaac gtcagcgggc gtggatcgcc atgctggtgg cgcaggatag
     5581 ccgttgtctg ttgctcgacg aaccgacctc ggcgctggat atcgcccacc aggttgatgt
     5641 gctggcgctg gtgcaccgtt taagtcagga gcgtggcctg acggtcattg ccgtgttgca
     5701 cgatatcaat atggcggcac gctactgtga ttatctggtc gccctgcgcg gtggtgaaat
     5761 gattgctcag ggaacgcctg cggaaattat gcgcggcgat accctcgaaa tgatttatgg
     5821 catcccgatg ggtattttgc cgcatccggc gggtgctgca cctgtgagtt ttgtttattg
     5881 atgagcggct tacctcttat ttcgcgccgt cgactgttaa cggcgatggc gctttctccg
     5941 ttgttatggc agatgaatac cgcccacgcg gcggctattg atcccaatcg tattgtggcg
     6001 ctggagtggt tgccggtgga attactgctg gcgctcggca tcgtgcctta cggcgtagcg
     6061 gataccatca actatcgcct gtgggtcagc gaaccaccat tgccggactc agtgatcgac
     6121 gtcggtttgc gcacagaacc taaccttgaa ctgctgaccg aaatgaaacc gtcgtttatg
     6181 gtctggtcgg caggatatgg cccttcagca gaaatgctgg cacgtattgc gccggggcgt
     6241 ggatttaact tcagcgacgg caaacagccg ctggcgatgg cgcgtaaatc actgacggaa
     6301 atggcagatt tacttaacct gcaaagcgca gcagaaacgc atttagcgca atatgaagac
     6361 tttatccgca gcatgaaacc ccgctttgtg aagcgtggtg cacgcccgtt attgctgacg
     6421 acgcttatcg atccgcgcca tatgttggtc ttcggtccaa acagcttgtt ccaggaaatt
     6481 cttgatgagt acggcatccc aaatgcctgg caaggggaaa ccaacttctg gggcagtacc
     6541 gccgtcagta tcgatcgtct ggcagcgtat aaagacgttg atgtgctctg ttttgatcac
     6601 gacaacagca aagacatgga tgcgctaatg gcaaccccgc tgtggcaggc catgccgttt
     6661 gtccgcgccg gacgctttca gcgcgtaccc gcagtctggt tttatggtgc gacgctctcg
     6721 gcaatgcact ttgtgcgcgt tctggataac gccatcggag gtaaagcgtg agtaaacgaa
     6781 ttgcgctttt cccggcgtta ttgctggcgc tgctggtgat tgtcgctacg gcgctgacct
     6841 ggatgaactt ttcgcaggcg ctgccgcgta gccagtgggc gcaggccgcc tggtcgccgg
     6901 atattgacgt catcgagcag atgatttttc actacagctt gctgccgcgt ctggcgattt
     6961 cgctgctggt gggcgcaggc ctggggctgg tgggcgtgct gtttcagcaa gtgctgcgta
     7021 atccgctggc ggaaccgacg acacttggcg ttgctacagg cgcgcaactg gggattaccg
     7081 tcactacgct ctgggcgatc ccaggggcga tggcgagtca gtttgctgcg ctggcgggcg
     7141 cttgcgttgt tggcttaatc gtctttggcg tcgcgtgggg gaaacggctt tcgccggtaa
     7201 cgctgatcct cgcggggctg gtagtgagcc tttattgtgg ggcaatcaat cagttactgg
     7261 ttatcttcca ccatgaccaa ctgcaaagca tgttcctgtg gagcaccgga acgctgacgc
     7321 aaaccgactg gggcggtgtt gagcgtttat ggccgcagtt gctgggcggt gtgatgctga
     7381 cgttgctgct acttcgcccg ttaaccctga tggggcttga tgatggcgtg gcgcgcaatc
     7441 tcgggctggc cttgtcgctc gcgcgtctgg cggcgctgtc gctggcgatt gtcatcagtg
     7501 cgctgctggt gaacgcggtg gggattatcg gctttatcgg gttgtttgcg ccgttgctgg
     7561 caaaaatgct gggggcgcgg cgtctgctgc cgcgactgat gctggcgtcg ttgattggtg
     7621 cgctgatcct ctggctttcc gatcaaatca tcctctggct gactcgcgtg tggatggaag
     7681 tgtccaccgg ttcggtcact gcgttgatcg gtgcgccgct gctactgtgg ctgttgccgc
     7741 gtttacgcag cattagcgcg ccggatatga aggtcaacga tcgtgtcgcg gctgaacgcc
     7801 aacatgtgct ggcgtttgcc ctcgcgggcg gcgtgctgct gttgatggct gtggtggtgg
     7861 cgctgtcgtt tggtcgtgat gcgcacggct ggatgtgggc gagcggggcg ttgctcgatg
     7921 atttaatgcc ctggcgctgg ccgcgaatta tggcggcgct atttgcgggc gtcatgctgg
     7981 cggtggcggg ctgtattatt cagcgactga ccggcaaccc gatggcaagc ccggaagtgc
     8041 tggggattag ctccggcgca gcgtttggcg tggtactgat gctgtttctg gtaccgggta
     8101 atgcttttgg ctggctgtta cccgcgggaa gtatcggtgc cgcggtgacg ctgttgatta
     8161 ttatgatcgc cgccgggcgc ggtggatttt ccccacaccg catgttactg gcggggatgg
     8221 cgttaagtac cgcattcacc atgcttttaa tgatgttgca ggcgagtggt gacccgcgaa
     8281 tggcgcaagt gctcacctgg atctctggtt cgacctacaa cgccaccgat gcgcaggtct
     8341 ggcgtaccgg aattgtgatg gtgattttgc tggcgattac cccgctgtac cgccgctggc
     8401 tgaccatttt acctctgggc ggtgataccg cacgagctgt gggaatggcg ctgacgccga
     8461 cgcgaattgc gctgctgctg ttagcggctt gcctgacggc gacctcgacg atgactattg
     8521 gaccgttgag ttttgttggt ttaatggcac cgcatattgc gcggatgatg ggttttcgac
     8581 ggacgatgcc acacatcgta atttcggcgc tggtgggtgg tttactgctg gtattcgctg
     8641 actggtgtgg gcggatggtg ctgtttccat tccagatccc ggcggggctg ctgtcgacct
     8701 ttatcggcgc gccatatttt atctatttgt tgagaaagca gagccgttaa ttgagtgcct
     8761 cgcccggtgg gcgaggcaaa attatcacaa cttcgcaaat acccgacgtg cagcatcgat
     8821 ggtgttattg atatcttcca tgctgtgcgc cacagacata aagcccgctt caaacgctga
     8881 cggtgccagg taaacacctt cgtccagcat catatggaag aaacgcttaa agcgttccac
     8941 gtcacaggcc atcacatcct gatagcacgt cacggactcg gcgtcggtaa agaaaatacc
     9001 gaacatgccg ccaacgtggt taacgaccag cggagttccg gcttcttctg ccgcttccag
     9061 cagaccttct gccagacgtg ttgtcagctc atccagcgtt tcgtgaacgc ccggctgcgc
     9121 gacttcattc agacaggcga aacccgctgc catcgcaatc gggttaccgg aaagcgtacc
     9181 cgcctgatag accggacccg tcggggccag cgcatccatt acatcacgac gaccaccgaa
     9241 tgcgcctacc ggcattccac cgccgatgat tttgccgagg caggttaaat ctggcactac
     9301 gccgtaataa tcctgtgcgc cagccagtgc cacgcggaag ccggtcatca cttcatcgat
     9361 gatcagcaac gcgccaaatt cgtcgcacag cgcacgcaga cctggcagga actctggctg
     9421 cggcggaaca cagttcatat tgcctgccac cggctcgacg ataatacagg caatctcttg
     9481 cgggtattgc tcaaacgcgg cgcgtacaga agccagatcg ttataagtac aggttaaggt
     9541 atgtttggcg aaatctgccg gaacacccgg cgagtttggc tggcctaacg tgagtgcgcc
     9601 agaaccggct ttcaccagca ggcagtcagc gtgaccgtgg taacaacctt caaatttaat
     9661 gattttgtcg cgaccggtaa aaccacgggc caggcggatg gcgctcatgg tcgcttcagt
     9721 gccggagttc accatgcgca ccatatccat ggtcgggacc agttcggtca ccagttgcgc
     9781 cattttcact tccatttcgg tcggtgcacc aaagcttaaa ccacgctcgg cggcttcaat
     9841 cacggcattg cggatagccg gatggttatg gcccagcacc atcggccccc aggaaccgac
     9901 ataatcgata taggctttgc catcaacatc gtacagataa gcgccgtccg ctttttcgat
     9961 aaacagtgga gtgccgccca cgccagtaaa ggcgcgaaca ggggagttca caccgccagg
    10021 gagcagctcg cgcgctgcgc tgtaaagatt ttcagactta ctcatggagg gttcctgatt
    10081 cgtagaaaaa gtgaatggct gctattctat gttattcata acaagttaaa tactcgtcaa
    10141 acatcaggct gcttgtactg gtgcaatcct gatttcttag agtataaaag ttttgtgcat
    10201 ttgaaacatt acgctttgca aaggattttc atggaacgtg cgagtaaaat gccgtcatct
    10261 tatttgtatg accaataagt gatcattgga tgaaaactga tactccctct ttagaaacac
    10321 cgcaggccgc gcgcctgcga cgcagacaac tgattcgcca acttcttgag cgcgataaaa
    10381 ccccgttagc cattttgttt atggcggcag tcgtcggcac gcttgtcggg ctggcagcgg
    10441 ttgcttttga caaaggtgtc gcctggttgc agaaccaacg tatggaggcg ctggtacata
    10501 ctgctgataa ttatccgctt ctgttaaccg tcgcttttct ctgttcggcg gtgctggcga
    10561 tgttcggcta ctttttggtg cgcaaatacg cgccggaagc aggtggttcg gggatcccgg
    10621 aaattgaagg ggcgctggaa gatcaacgtc ccgttcgctg gtggcgtgtg ttgccggtga
    10681 aattctttgg cgggctgggg acactcggcg gaggcatggt gctggggcgc gaagggccaa
    10741 ccgtgcagat cggcggcaat atcggtcgca tggtactcga tattttccgc ctgaaaggtg
    10801 atgaagctcg ccatacgctg ctggcaaccg gtgctgctgc ggggctggct gcggccttta
    10861 acgcgccgct ggcgggtatt ttgtttatta tcgaagagat gcgtccgcag tttcgctata
    10921 cgttaatttc gattaaagcg gtatttattg gcgtcattat gtcgaccatt atgtaccgga
    10981 tttttaatca tgaagttgcg ttgattgacg tcggtaaact ttctgatgcg ccgcttaata
    11041 cgctgtggct ttatctgatc ctcggtatta tttttggcat tttcggccct atttttaata
    11101 aatgggtgct ggggatgcag gatttgctgc accgtgtgca cggcggcaat attaccaaat
    11161 gggtgctaat gggcggtgcg attggcggtc tgtgtggatt gctggggttt gtggcaccag
    11221 caacgtcggg cggcggtttt aacctgattc ctatcgctac cgcagggaat ttcagcatgg
    11281 gaatgctggt gtttatcttc gtcgcgcggg tcattaccac cttactgtgc ttctcttccg
    11341 gcgcgccggg cggtattttt gccccgatgc tggcgctggg tactgtgctg ggaaccgctt
    11401 tcggaatggt tgtcgttgag ctgtttccgc aatatcacct tgagtcgggg acttttgcta
    11461 ttgccggaat gggggcgtta ctggcggcat ctattcgcgc gccgttaacg gggatcattt
    11521 tggttctgga gatgaccgat aactaccagc tcattttgcc aatgattatt accggtcttg
    11581 gcgcaacact attagcgcaa tttaccggcg ggaaaccgct atattcggag attcttgcgc
    11641 gcacgctggc aaaacaggaa gctgagcaac tggcgcgaag caaggccgca tcagccagcg
    11701 agaatacttg aacgaaatac cagggtatta gataatggcg attattattg ggttagaatt
    11761 tgcccaattg ccgatgtcgt ttggagcaaa atatgagtga tgacgtagca ctgccgctgg
    11821 agtttaccga cgcagcagcc aacaaagtta aaagcctgat cgctgacgaa gataacccga
    11881 atctgaaatt acgcgtgtat atcaccggtg gcggttgcag cggcttccag tatggtttca
    11941 cctttgatga tcaggtgaac gaaggcgata tgaccatcga aaaacagggc gttggcctgg
    12001 tggttgatcc gatgagcctg caatatctgg tcggtggttc cgttgactat accgaaggac
    12061 tggaaggttc tcgtttcatc gtgaccaacc cgaacgcgaa aagcacctgc ggttgcggtt
    12121 cttcctttag catctaatct gttgttcatg tgccggataa actatccggc acatcttctc
    12181 tcttcaacgc ccattctcat ccagcgcaaa cgtcggtagc ttaagatgcc aacgaatagc
    12241 cgccagccga atcaatagcg tcacgaccat gcccatcata ctggctgttt ccagtggtac
    12301 ggaaaatgtg taataagccg tagcgtggac aataccgccg ataatacagg cagttgcgta
    12361 gatttctgta cgtaaaatca tggggatttc gcgggccagt acatcacgaa tgatcccgcc
    12421 gccaacgcca gtaatgacgc ccatacaaac cgcgattaac ggaccggctt ccgcattaaa
    12481 ggctttattc acgccaatgc cgacaaacac cgccagacca acggcgtcca gcaccggcaa
    12541 catccatttt ggtaagcgtc ttggctggcg caccagcacg atggtcagca tgctggttac
    12601 cattgcaacg accagatcgg tgggatcttt cacccaaaat accgggccgt gatccagcgc
    12661 catgtcgcga attgtcccgc cgcctacagc ggtaaccacg cccagtacca gaacaccaaa
    12721 agggtccata cgcaattttc cggctaacaa aacgccggag atggcaaata ccgctgtgcc
    12781 gactatatcc agccaataga cgagcattgt tcaatcccca ctgagcacct aaaaaggtca
    12841 ggcgctaatc tacctgtgaa agcgcattac agagctgttg tgcagcgagg ataatacgtg
    12901 ggcttgcacg tcaaaccagt cactcgtgag aggaataacg ggaattttga gctgttcacc
    12961 ccagtattgt ttgattttag gaatttggtc cggtccgcct gtaatgacaa tcgcctgtgg
    13021 cgagcgtgct aacacctgtt cgcggctaac ttgcggccaa ggaacccggc tgtctttaaa
    13081 gatgttttct ccgccacaaa cttcgagtac ctggttttga atcgactctt ttccactggt
    13141 aaatggcgga ttaatgccga attgcagaaa aacacgtttt ttaggtttat cagcatattg
    13201 cgctttcaat tgcgcgtact gatccagcag ggattgcgcg gcttgttcgg ccttatccgg
    13261 ttgcggactc cagggggcca gttgacgtaa cgcattggca atttgttcaa tgcttgtcgc
    13321 atcgacccac atcactttta ttcccggcga agccagctgg tcaacctgcc gctcggcatt
    13381 acctccacgc caggcaatca ccagatcggg tttcagcgcg acaatgcgtt ccagattcat
    13441 cccctgccag gtggaaacct gctcaatctt ttgcgcttgt ggaggatagt cggaatagct
    13501 gctgacccca accggcgtga tcccggcggc aaaggcaagt tcagtgttgg cgggagaaag
    13561 cgtgatgacg cgcggtgccg cgttgagcca cagtggcgcg agaaaagaca gggcgaccag
    13621 cgccctgaac agtgacttag ccatgtgcca gtttctgcac cagtgactca accatcaggc
    13681 tggactgttt agcggcaaca gccaggaact catcgaagct aagatgagac tgttgatcgg
    13741 ccacgtcgga gatggcgcgt acgacaacaa acgggacgtt gaaattgtgg cagacatggg
    13801 cgattgccgt cgcttccatc tctacagcaa tggcctgtgg gaagttgtgg cggattttcg
    13861 ccagaccaac agaaccgttg atgaaagcgt cgccgctaac aatcaggcca cgtacagcgt
    13921 taagattcag ttcggcaatg caggcctcag cggcagcgat cagtttatcg tcagctttaa
    13981 agcctgccgg acagcctggt aactgaccgt attcataacc aaatgccgtg acatccgcgt
    14041 cgtgataacg tgcttcgtcc gagacaacga tatcgcccac tttcaacgtt ggtgccaggc
    14101 caccggcaga accggtgtta ataatcacat ctggcttgcc gtgttccaac agcaaagtgg
    14161 cacccagcgc cgcagcgact ttaccgatgc ccgatttcag aagcgcaacc tcggttccat
    14221 tcagttggcc ggtatagatt tcgcaaccgc cgagactgat agtttgacga ttttcgattt
    14281 tgtcacgcag cagcgtaact tcttcttcca ttgcaccaat gatgccgatt ttcatagatt
    14341 tactcgcgat aagcccgatt tgaaggcata gtttaccatg cgcttacggg gaagcgtatt
    14401 tctcacgcgg gagaggacat ggcacagatt gatttccgaa aaaaaataaa ctggcatcgt
    14461 cgttaccgtt caccgcaggg cgttaaaacc gaacatgaga tcctgcggat cttcgagagc
    14521 gatcgcgggc gtatcatcaa ctctccggca attcgtcgtc tgcaacaaaa gacccaggtt
    14581 tttccactgg agcgcaatgc cgccgtgcgc acgcgtctta cccactcgat ggaagtccag
    14641 caggtggggc gctacatcgc caaagaaatt ctaagccgta tgaaagagct taaattactg
    14701 gaagcatacg gcctggatga actgaccggc ccctttgaaa gcattgttga gatgtcatgc
    14761 ctgatgcacg atatcggcaa tccgccgttt ggtcattttg gcgaagcggc gataaatgat
    14821 tggtttcgcc agcgtttgca cccggaagat gccgaaagcc agcctctgac tgacgatcgc
    14881 tgcagcgtgg cggcactacg tttacgggac ggggaagaac cgcttaacga gctgcggcgc
    14941 aagattcgtc aggacttatg tcattttgag gggaatgcac aaggcattcg tctggtgcat
    15001 acattgatga ggatgaatct cacctgggca caggttggcg gtattttaaa atatacccgt
    15061 ccggcgtggt ggcgtggcga aacgcctgag acacatcact atttaatgaa aaagccgggt
    15121 tattatcttt ctgaagaagc ctatattgcc cggttgcgta aagaacttaa tttggcgctt
    15181 tacagtcgtt ttccattaac gtggattatg gaagcagccg acgacatctc ctattgtgtg
    15241 gcagaccttg aagatgcggt agagaaaaga atatttaccg ttgagcagct ttatcatcat
    15301 ttgcacgaag cgtggggcca gcatgagaaa ggttcgctct tttcgctggt ggttgaaaat
    15361 gcctgggaaa aatcacgctc aaatagttta agccgcagta cggaagatca gttttttatg
    15421 tatttacggg taaacaccct aaataaactg gtaccctatg cggcacaacg atttattgat
    15481 aatctgcctg cgattttcgc cggaacgttt aatcatgcat tattggaaga tgccagcgaa
    15541 tgcagcgatc ttcttaagct atataaaaat gtcgctgtaa aacatgtgtt tagccatcca
    15601 gatgtcgagc agcttgaatt gcagggctat cgggtcatta gcggattatt agagatttat
    15661 cgtcctttat taagcctgtc gttatccgac tttactgaac tggtagaaaa agaacgggtg
    15721 aaacgtttcc ctattgaatc gcgcttattc cacaaactct cgacgcgcca tcggctggcc
    15781 tatgtcgagg ctgtcagtaa attaccgtca gattctcctg agtttccgct atgggaatat
    15841 tattaccgtt gccgcctgct gcaggattat atcagcggta tgaccgacct ctatgcgtgg
    15901 gatgaatacc gacgtctgat ggccgtagaa caataaccag gcttttgtaa agacgaacaa
    15961 taaattttta ccttttgcag aaactttagt tcggaacttc gcgctataaa acgaatctga
    16021 agaacacaga aattttgcgt tatctgttaa tcgagactga aatacatgaa aaaaaccaca
    16081 ttagcactga gtgcactggc tctgagttta ggtttggcgt tatctccgct ctctgcaacg
    16141 gcggctgaga cttcttcagc aacgacagcc cagcagatgc caagccttgc accgatgctc
    16201 gaaaaggtga tgccttcagt ggtcagcatt aacgtagaag gtagcacaac cgttaatacg
    16261 ccgcgtatgc cgcgtaattt ccagcagttc ttcggtgatg attctccgtt ctgccaggaa
    16321 ggttctccgt tccagagctc tccgttctgc cagggtggcc agggcggtaa tggtggcggc
    16381 cagcaacaga aattcatggc gctgggttcc ggcgtcatca ttgatgccga taaaggctat
    16441 gtcgtcacca acaaccacgt tgttgataac gcgacggtga ttaaagtcca gctgagcgat
    16501 ggccgtaagt tcgacgcgaa gatggtcggc aaagatccgc gctctgatat cgcgctgatc
    16561 caaatccaga acccgaaaaa cctgaccgca attaagatgg cggattctga tgcactgcgc
    16621 gtgggtgatt acaccgtagc gattggtaac ccgtttggtc tgggcgagac ggtaacttcc
    16681 gggattgtct ctgcgctggg gcgtagcggc ctgaatgctg aaaactacga aaacttcatc
    16741 cagaccgatg cagcgatcaa ccgtggtaac tccggtggtg cgctggttaa cctgaacggc
    16801 gaactgatcg gtatcaacac cgcgatcctc gcaccggacg gcggcaacat cggtatcggt
    16861 tttgcaatcc cgagcaacat ggtgaaaaac ctgacctcgc agatggtgga atacggccag
    16921 gtgaaacgcg gtgagctggg tattatgggg actgagctga actccgaact ggcgaaagcg
    16981 atgaaagttg acgcccagcg cggtgctttc gtaagccagg ttctgcctaa ttcctccgct
    17041 gcaaaagcgg gcattaaagc gggtgatgtg atcacctcac tgaacggtaa gccgatcagc
    17101 agctttgccg cactgcgtgc tcaggtgggt actatgccgg tgggcagcaa actgaccctg
    17161 ggcttactgc gcgacggtaa gcaggttaac gtgaacctgg aactgcagca gagcagccag
    17221 aatcaggttg attccagctc catcttcaac ggcattgaag gcgctgagat gagcaacaaa
    17281 ggcaaagatc agggcgtggt agtgaacaac gtgaaaacgg gcactccggc tgcgcagatc
    17341 ggcctgaaga aaggtgatgt gattattggc gcgaaccagc aggcagtgaa aaacatcgct
    17401 gaactgcgta aagttctcga cagcaaaccg tctgtgctgg cactgaacat tcagcgcggc
    17461 gacagcacca tctacctgtt aatgcagtaa tctcactcaa ccccttcctg aaaacgggaa
    17521 ggggttctcc ttacaatctg tgaacttcac cacaactcca tacatcttca tcatccttta
    17581 ggcatttgca caatgccgta cgttacgtac ttccttatgc taagccgtgc ataacggagg
    17641 acttatggct ggctggcatc ttgataccaa aatggcgcag gatatcgtgg cacgtaccat
    17701 gcgcatcatc gataccaata tcaacgtaat ggatgcccgt gggcgaatta tcggcagcgg
    17761 cgatcgtgag cgtattggtg aattgcacga aggtgcattg ctggtacttt cacagggacg
    17821 agtcgtcgat atcgatgacg cggtggcacg tcatctgcac ggtgtgcggc aagggattaa
    17881 tctaccgtta cggctggaag gtgaaattgt cggcgtaatt ggcctgacag gtgaaccaga
    17941 gaatctgcgt aaatatggcg aactggtctg catgacggct gaaatgatgc tggaacagtc
    18001 gcggttgatg cacttgttgg cgcaggatag ccgtttgcgg gaggaactgg tgatgaacct
    18061 gattcaggca gaggagaata ctcccgcact tactgaatgg gcgcaacggc tggggatcga
    18121 tctcaatcaa ccgcgagtgg tggctattgt tgaggtcgac agcggtcagc ttggtgtgga
    18181 cagcgcaatg gcggagttac aacaactgca aaacgcgctg actacgcccg agcgtaataa
    18241 tctggtggcg attgtctcgc taaccgaaat ggtggtgttg aaaccggcgt tgaactcttt
    18301 tgggcgctgg gatgcagaag atcatcgtaa gcgagttgaa caactgatta cccgcatgaa
    18361 agagtacggc cggctgcgtt ttcgcgtttc actgggcaac tattttaccg gtcctggcag
    18421 tattgcccga tcctatcgta cggcgaaaac gacgatggtg gtgggtaaac agcggatgcc
    18481 agaaagtcgc tgctattttt atcaggatct gatgttacct gtgttactcg acagtttgcg
    18541 tggcgactgg caggccaacg aactggcgcg accgctggcg cggctgaaag cgatggacaa
    18601 taacggcttg ctgcgacgaa cgctggcggc gtggtttcgt cacaatgtgc aaccgctggc
    18661 aacgtcaaag gcgttgttta tttatcgtaa taccctggag tatcggctta atcgtatatc
    18721 ggaactgacc gggcttgatt tgggcaattt tgatgacagg ttgctgctgt atgtggcgtt
    18781 acaactggat gaagagcggt aggttatgtg ttaaggttga ggcggcgctg tctcatttcc
    18841 ctcagcccga aggggagagg ggaatgcccg aattaaccgt caattcggga ttatttacgc
    18901 gttagttttt ccagatcggc ttcaatctcg ctgatcttat tggttacgac tgactccagg
    18961 tgacgtaagt cgtcgaggat cttacgctta agatcaactt cgctgcggtc acgctggcag
    19021 atttgatcaa gctcatcaat gatataccgt agattcgggc tgatttcctg gacttcttta
    19081 taaccctgac ccacaccatc agcgacgacc gttttacgct gacgcggata tttaaactta
    19141 acgctcttgg cgaaaaactc gcctttgtct ttctggaaat agattttcag aatatcgttg
    19201 ttggcttcct gccggaggct gtaacgatca atttcttcag gattggtaat acccagactt
    19261 ttcagattgt cgtacatagc gttacctcaa aatgagtcag taaattgtgc ttattttagc
    19321 atttggccta gcccgcccgg ctcgactgtg atcgcagaaa gcctttttct tccagacttc
    19381 tatcatggcg taaaaatcaa aaaattacct gctttattgt ggtgataaaa ttcacgttct
    19441 acaccagttc cagcatcgtt acttccgggc ggcagttcag acgcaaacca tacaaactgc
    19501 ccacgccacg ggttgtgtag atgtgtcgtt cgccaaaggc atttaatccg gcgacgtaac
    19561 gtttatcttc gacaggggca aaaggttctc caaccaacgg tacgcgcagt tgcccgccgt
    19621 gagtatggcc gcacagcatc agatcccagg gttcatcacg catgacttct ttgctgtcgg
    19681 gattatgtgc cagcatcagt cttggcagat ttgcttcgct ggcgggaggt ggtttgcatt
    19741 gtccggccca taagtcaccg gtgcctacca gttcgaattg cctgttcggc gtggcgatca
    19801 ccgtagcctg gttaaacaac accgtgatgc ccactgattt caacgtctcg ccaattaagt
    19861 gatttttttc tgtaccaaca gggcgatcgt ggttgccgaa gcaggcaaac gtcggcgcac
    19921 attcggcaag gggggagagg acatcactaa acgccgaaaa attcagcggc atatcaaata
    19981 atacgtaatc gccgcccagt aatatcaaat cgggcttttg ttctatgcca agagcaatcg
    20041 cgtcagaaat caggcttaaa ggaacaaaac gagagtaatg gagatcggcc agaaaaagaa
    20101 ttttgaatgg tgctgcgttg tctttaaaaa aggcgaggcg gtgacggatt aattcaaacc
    20161 agcctggctc ccagtaatgc atataaccga aacctgagct cgtggctatc gtcgcggcag
    20221 tagcctgcaa caatcggcgg cgtgaaatca tcgctcatcc ctgcaaaaaa aatcgggcag
    20281 cgtcgtgctg cccgtgtgca tacttttagt cgatggtacg cagcagttcg ttaatgccga
    20341 ctttgccgcg agttttcgcg tcaactttct taacgataac cgcacagtag aggctgtatt
    20401 tgccatcttt tgacggcaga ttacctgaaa caaccacaga ccccgccgga acgtgaccgt
    20461 agtggatttc gccggtttca cggtcgtaaa tacgggtgct ctgaccaatg tatacgccca
    20521 tggaaatgac ggaaccttct tcgacaatca ccccttcaac tacttcagag cgtgcgccaa
    20581 tgaagcagtt atcttcaatg atggttgggt tagcctgcag cggttccagc acgccgccga
    20641 tgcccacgcc accggaaagg tggacgtttt taccaatctg cgcacaagaa ccgacggtcg
    20701 cccaggtatc aaccatggtg ccttcatcaa catatgcgcc gatgttgacg taagacggca
    20761 tcagcacggt gttacgggca ataaacgcac cctgacgtac cgccgctggt ggcacaacgc
    20821 ggaagccttc tttctggaaa cgtgcttcgt cgtagtcggc gaatttcatt ggcactttgt
    20881 cgaagtagcg gctttctgcc ccttcgatca cctgattatc attaatacgg aaagagagca
    20941 gcaccgcttt tttcaaccac tgatgcgtca cccactgacc gtcaattttt tccgctacac
    21001 gcagtgcacc ggaatccagc agggcgatca cctgatttac cgcttcgcgg gtaacggtgt
    21061 ctgcgttggt tggcgtgatc tcggcacggc gttcaaaagc ggtttcaata atgttctgta
    21121 actgctgcat tgttaaactc ttttcatatc agtaaacaca tcacccttta tcgtttggat
    21181 tgagggcctc tgtcaaccgc tgatgcactt cctgctgcaa ctcgttatta agcgcacgcc
    21241 ggtcagcggt ggcaattatg aataaatctt ctactcgctc gccaatggtt gtaattcggg
    21301 caccatgaag cgaaattccc agatcggcaa aaattttccc gactctcgcc agcagtcctg
    21361 gctggtcgag cgcgataagc tcaaggaacg atttgcggtc ggtatgggtc ggcaaaaacg
    21421 ttacttcggt ttcaacagta aaatggcgta atttggcggg ttggcgacgg ggctgcggtg
    21481 gctgccagct actttgcgtc agtacttgct ccagaccaaa ccgaataacc tcatgacgat
    21541 ctgcggacag cgggctgcca tcgggttcca gcacgataaa ggtatccatc gccataccgt
    21601 cgcgagtggt gaaaatttgt gcgtcgtgaa cacttaaatt gcggcggtct aattcggcac
    21661 agacggcggc aaacagatag gggcggtccg ggctccagat aaaaatctcg gtgcctccac
    21721 gcgtagcctg cgggctaagc aataccagtg gtttgcttaa atcatgctgt aataaatggc
    21781 gggcatgcca ggccagttga tttgggctat ggcggacaaa atagttagca cgacagcgtg
    21841 accaaatttg gtgcagcgcc tcttcgtcga tgttatccat gcgcagtagt gccagtgcct
    21901 ggagttggtg atggcgaacc cgttcgcgca tatccggcgt gttttgcatt ccgcgtcgta
    21961 gctgcttttc ggtggcaaaa tagagttcgc gcaacagact ttgcttccag ctattccaca
    22021 gcgtttcgtt ggtggcgcaa atgtcagcca ctgtcaggca taccagatag cgcagacgat
    22081 tttccgtttg aacttcttcg gcaaactgct tgatgacttc agggtcctga atatcgcggc
    22141 gttgtgctgt caccgacatc agcaagtgct ggcgaaccag ccaggcaacc agctgtgttt
    22201 cgcgtgagtt cagcccgtgg agttctgcaa aatgcactac atcctgagca ccgagaatgg
    22261 agtggtcgcc gccgcgtcct ttggcgatat cgtgaaacag cgcggcgatg aaaatcagct
    22321 cagttgacgg caggcgcggc cagacgtcca cacacaacgg atggcgctgg cgcgtttctt
    22381 cactggcaaa actctccagt ttcagcatca cgcggatagt atgttcatcc accgtgtagg
    22441 cgtggaacag atcaaactgc atctgcccga cgatatgcga ccattgcggc atatacgcgc
    22501 cgagcacgct atggcgatgc attggcaata gcccgcgccg caccgctccg gggtgacgca
    22561 gaatgctcaa aaacagtttt cgtgcttccg gaatattaca cagcggttgt tgcagatggc
    22621 gacgggcatg gcgtaactgg cgcagcgtgg tggagtaaat gccggtgatc gcactgttgc
    22681 gcaccatggt gtagaacata cgcaagatgg cttccggctg gcgcataaat agtgtttcat
    22741 cacgcaggtc gattagcgta ccgcgtagct gaaactcatc gtcgattgga cgtggttttt
    22801 cgtcggcagg aagggcgagg atggcttcat cgaacagttg cagcagcatc tggttgagtt
    22861 cactgacgcg gcgtgtaacg cggaagtaat ccttcatcat ccgctcgacc ggttcgttgc
    22921 cttcaccact gtaattcaga cgctgagcga cgctaagttg gcgatcgaat aacaggcgat
    22981 tatcgtaacg gctgacgacc agatgcaggg caaagcgaat acgccacagt atatgcagac
    23041 attcgtttaa ttccgcccgc tccgctgagg ttaagaagcc aaacccaacc atttcatcca
    23101 gcgatgttgc gccaaaatga cggcgggcca cccattgcag agtgtggata tcgcgcaagc
    23161 cgccagggct acttttgatg tctggttcaa gattgtagct ggtgccgtgg taacgctgat
    23221 ggcgctggtt ctgttcttca actttcgccg cgtagaactt gtcggaaggc cagaatcctt
    23281 cgctaaaaat atgtttttgc agttcgagga agagcgcaac atcgccaatt aataagcggg
    23341 attcgattaa attggtggcg acggttaaat ccgataaccc ttccagcatg cactcttcaa
    23401 gcgtgcgcac actatgaccg acttccagct ttacatccca gagcagcgtt aacagctcgc
    23461 ccactttttg cgcctgatcg tccgggagct ttttacggct taaaatcagt aaatcgatgt
    23521 cagaaagtgg atgcagctcg ccacgaccgt agccaccgac ggcgaccaat gccaggtcgg
    23581 caatctggct gaatcccgct tcaatccata atcgttgcag gagctggtcg ataaactcgg
    23641 tgcgcgcctc aatcaactgt tctgcagaga tcccattgtc aaaggcatca cccagccaac
    23701 gctggaaagt atcgatatgg gcttttatcc caccgacggt taattcatca cggggccaga
    23761 cgcatggatt ttgcggttga ccgggcaggg tggggagagc ggtgtttgcg tactgttctg
    23821 gaagggtatt cattgtgcgc cactcataag attaaattat cgcattaaaa aagccggcat
    23881 tcgccggctt catcttattc gtcgtgcgag attatcgccg ggatggtgtc atccttgcgt
    23941 agcgtcagaa tttcgcagcc gttatcagtc accacaatag tatgctcata ttgtgcagac
    24001 aagctgcgat ctttggtttt taccgtccag ccatctttca tggtgcggat ctctttttta
    24061 cccgcgttga ccattggctc gatggtgaac gtcatcccag gtttcagtac gacgttggtt
    24121 tcacgggagt catagtgcag cacctgcggt tcttcatgga agccgcgacc aataccgtgt
    24181 ccgcaatatt cacgaacgac ggagaagcct tctgcttcga caaacttctg aatcgccgca
    24241 ccgatttcgc gcaggttaat gcctggtttg accatgcgca gcgccaggta caggctttct
    24301 tgcgtgatgc ggcacagacg ttcgcccatg atggtcggct taccgacgat aaacattttc
    24361 gaggtatcgc cgtggaaacc atctttgatt acggtgacat caatgttaac gatatcgcca
    24421 tctttcagca gcttcgcatc gtccgggata ccgtggcaca ccacttcatt aatagagatg
    24481 caaacggatt tcggatagcc gtgatagccg aggcaggcag aaaccgcgtg ttgttcatta
    24541 acaatgtaat cattacagat gcgatccagc tcgccggtgc tgacgcccgg tttaacatac
    24601 ggttcgatca tctccagcac ttcggcagcc agtcggccag cgacgcgcat tttttcgata
    24661 tcttctgggg tcttgattga gatagccatt aattctgtcc atcagcgtcg gtgataccga
    24721 caatatatat gtaagtgccg tcaatggtat cacacccggg caaattgaga atcattctga
    24781 atttcgccaa acgtgccact gaacgttttc tataatagaa aattcaacgt ctgatgctgt
    24841 acacagcgcc aacaattatt ggtgtccatg acgtatttgt ggtataaagc gcgccggact
    24901 tccgatccat ttcgtataca cagactggac ggaagcgaca atctcacttt gtgtaacaac
    24961 acacacgtat cggcacatat tccggggtgc cctttggggt cggtaatatg ggatacgtgg
    25021 aggcataacc ccaactttta tatagaggtt ttaatcatgg caactgtttc catgcgcgac
    25081 atgctcaagg ctggtgttca cttcggtcac cagacccgtt actggaaccc gaaaatgaag
    25141 ccgttcatct tcggtgcgcg taacaaagtt cacatcatca accttgagaa aactgtaccg
    25201 atgttcaacg aagctctggc tgaactgaac aagattgctt ctcgcaaagg taaaatcctt
    25261 ttcgttggta ctaaacgcgc tgcaagcgaa gcggtgaaag acgctgctct gagctgcgac
    25321 cagttcttcg tgaaccatcg ctggctgggc ggtatgctga ctaactggaa aaccgttcgt
    25381 cagtccatca aacgtctgaa agacctggaa actcagtctc aggacggtac tttcgaaaag
    25441 ctgaccaaga aagaagcgct gatgcgcact cgtgagctgg agaaactgga aaacagcctg
    25501 ggcggtatca aagacatggg cggtctgccg gacgctctgt ttgtaatcga tgctgaccac
    25561 gaacacattg ctatcaaaga agcaaacaac ctgggtattc cggtatttgc tatcgttgat
    25621 accaactctg atccggacgg tgttgacttc gttatcccgg gtaacgacga cgcaatccgt
    25681 gctgtgaccc tgtacctggg cgctgtagct gcaaccgtac gtgaaggccg ttctcaggat
    25741 ctggcttccc aggcggaaga aagcttcgta gaagctgagt aataaggctt gataactccc
    25801 ccaaaatagt tcgagttgca gaaaggcggc aagctcgaga attcccggga gcttacatca
    25861 gtaagtgacc gggatgagcg agcgaagata acgcatctgc ggcgcgaaat atgaaggggg
    25921 agagccctta tagaccaggt agtacacgtt tggttagggg gcctgcatat ggcccccttt
    25981 ttcactttta tatctgtgcg gtttaatgcc gggcagatca catctccgag gattttagaa
    26041 tggctgaaat taccgcatcc ctggtaaaag agctgcgtga gcgtactggc gcaggcatga
    26101 tggattgcaa aaaagcactg actgaagcta acggcgacat cgagctggca atcgaaaaca
    26161 tgcgtaagtc cggtgctatt aaagcagcga aaaaagcagg caacgttgct gctgacggcg
    26221 tgatcaaaac caaaatcgac ggcaactacg gcatcattct ggaagttaac tgccagactg
    26281 acttcgttgc aaaagacgct ggtttccagg cgttcgcaga caaagttctg gacgcagctg
    26341 ttgctggcaa aatcactgac gttgaagttc tgaaagcaca gttcgaagaa gaacgtgttg
    26401 cgctggtagc gaaaattggt gaaaacatca acattcgccg cgttgctgcg ctggaaggcg
    26461 acgttctggg ttcttatcag cacggtgcgc gtatcggcgt tctggttgct gctaaaggcg
    26521 ctgacgaaga gctggttaaa cacatcgcta tgcacgttgc tgcaagcaag ccagaattca
    26581 tcaaaccaga agacgtatcc gctgaagtgg tagaaaaaga ataccaggta cagctggata
    26641 tagcgatgca gtctggtaag ccgaaagaaa tcgcagagaa aatggttgaa ggccgcatga
    26701 agaaattcac cggcgaagtt tctctgaccg gtcagccgtt cgttatggaa ccaagcaaaa
    26761 ctgttggtca gctgctgaaa gagcataacg ctgaagtgac tggcttcatc cgcttcgaag
    26821 tgggtgaagg catcgagaaa gttgagactg actttgcagc agaagttgct gcgatgtcca
    26881 agcagtctta attatcaaaa aggagccgcc tgagggcggc ttctttttgt gcccatcttg
    26941 taaattcagc taacccttgt ggggctgcgc tgaaaagcga cgtacaatgt cgctagtatt
    27001 aattcatttc aatcgttgac agtctcagga aagaaacatg gctaccaatg caaaacccgt
    27061 ctataaacgc attctgctta agttgagtgg cgaagctctg cagggcactg aaggcttcgg
    27121 tattgatgca agcatactgg atcgtatggc tcaggaaatc aaagaactgg ttgaactggg
    27181 tattcaggtt ggtgtggtga ttggtggggg taacctgttc cgtggcgctg gtctggcgaa
    27241 agcgggtatg aaccgcgttg tgggcgacca catggggatg ctggcgaccg taatgaacgg
    27301 cctggcaatg cgtgatgcac tgcaccgcgc ctatgtgaac gctcgtctga tgtccgctat
    27361 tccattgaat ggcgtgtgcg acagctacag ctgggcagaa gctatcagcc tgttgcgcaa
    27421 caaccgtgtg gtgatcctct ccgccggtac aggtaacccg ttctttacca ccgactcagc
    27481 agcttgcctg cgtggtatcg aaattgaagc cgatgtggtg ctgaaagcaa ccaaagttga
    27541 cggcgtgttt accgctgatc cggcgaaaga tccaaccgca accatgtacg agcaactgac
    27601 ttacagcgaa gtgctggaaa aagagctgaa agtcatggac ctggcggcct tcacgctggc
    27661 tcgtgaccat aaattaccga ttcgtgtttt caatatgaac aaaccgggtg cgctgcgccg
    27721 tgtggtaatg ggtgaaaaag aagggacttt aatcacggaa taattcccgt gatggataaa
    27781 taagggtaag attccgcgta agtatcgcgg gggcgtaagt ctggttataa ggcgttattg
    27841 ttgcaggcag tttggtcacg gccagcgcgc agcaaccgga gcgtacaaaa gtacgtgagg
    27901 atggcgagca ctgctcgggg ccaaaatggc aaataaaata gcctaataat ccagacgatt
    27961 acccgtaata tgtttaatca gggctatact tagcacactt ccactgtgtg tgactgtctg
    28021 gtctgactga gacaagtttt caaggattcg taacgtgatt agcgatatca gaaaagatgc
    28081 tgaagtacgc atggacaaat gcgtagaagc gttcaaaacc caaatcagca aaatacgcac
    28141 gggtcgtgct tctcccagcc tgctggatgg cattgtcgta gaatattacg gcacgccgac
    28201 gccgctgcgt cagctggcaa gcgtaacggt agaagattcc cgtactctga aaatcaacgt
    28261 gtttgatcgt tcaatgtctc cggccgttga aaaagcgatt atggcgtccg atcttggcct
    28321 gaacccgaac tctgcgggta gcgacatccg tgttccgctg ccgccgctga cggaagaacg
    28381 tcgtaaagat ctgaccaaaa tcgttcgtgg tgaagcagaa caagcgcgtg ttgcagtacg
    28441 taacgtgcgt cgtgacgcga acgacaaagt gaaagcactg ttgaaagata aagagatcag
    28501 cgaagacgac gatcgccgtt ctcaggacga tgtacagaaa ctgactgatg ctgcaatcaa
    28561 gaaaattgaa gcggcgctgg cagacaaaga agcagaactg atgcagttct gatttcttga
    28621 acgacaaaaa cgccgctcag tagatccttg cggatcggct ggcggcgttt tgctttttat
    28681 tctgtctcaa ctctggatgt ttcatgaagc aactcaccat tctgggctcg accggctcga
    28741 ttggttgcag cacgctggac gtggtgcgcc ataatcccga acacttccgc gtagttgcgc
    28801 tggtggcagg caaaaatgtc actcgcatgg tagaacagtg cctggaattc tctccccgct
    28861 atgccgtaat ggacgatgaa gcgagtgcga aacttcttaa aacgatgcta cagcaacagg
    28921 gtagccgcac cgaagtctta agtgggcaac aagccgcttg cgatatggca gcgcttgagg
    28981 atgttgatca ggtgatggca gccattgttg gcgctgctgg gctgttacct acgcttgctg
    29041 cgatccgcgc gggtaaaacc attttgctgg ccaataaaga atcactggtt acctgcggac
    29101 gtctgtttat ggacgccgta aagcagagca aagcgcaatt gttaccggtc gatagcgaac
    29161 ataacgccat ttttcagagt ttaccgcaac ctatccagca taatctggga tacgctgacc
    29221 ttgagcaaaa tggcgtggtg tccattttac ttaccgggtc tggtggccct ttccgtgaga
    29281 cgccattgcg cgatttggca acaatgacgc cggatcaagc ctgccgtcat ccgaactggt
    29341 cgatggggcg taaaatttct gtcgattcgg ctaccatgat gaacaaaggt ctggaataca
    29401 ttgaagcgcg ttggctattt aacgccagcg ccagccagat ggaagtgctg attcacccgc
    29461 agtcagtgat tcactcaatg gtgcgctatc aggacggcag tgttctggcg cagctggggg
    29521 aaccggatat gcgtacgcca attgcccaca ccatggcatg gccgaatcgc gtgaactctg
    29581 gcgtgaagcc gctcgatttt tgcaaactaa gtgcgttgac atttgccgca ccggattatg
    29641 atcgttatcc atgcctgaaa ctggcgatgg aggcgttcga acaaggtcag gcagcgacga
    29701 cagcattgaa tgccgcaaac gaaatcaccg ttgctgcttt tcttgcgcaa caaatccgct
    29761 ttacggatat cgctgcgttg aatttatcgg tactggaaaa aatggatatg cgcgaaccac
    29821 aatgtgtgga cgatgtgtta tctgttgatg cgaacgcgcg tgaagtcgcc agaaaagagg
    29881 tgatgcgtct cgcaagctga ggataatccg gctacagaga gtctcgctat ttgttagcgt
    29941 agggcttcag tgatatagtc tgcgccatct gatcgtaagt agttggcttt ataaggtcag
    30001 gtatgccgtg gttttgcacg gctttttttg tataggcttc agtattcctg agtaccgtaa
    30061 accctgtcag ggaataaaaa acgcgtgatg ttgtctgcta ctcaaccact tagcgaaaaa
    30121 ttgccagcgc atggctgccg tcatgttgcg atcattatgg acggcaatgg tcgctgggca
    30181 aaaaagcaag ggaagattcg tgcctttggg cataaagccg gggcaaaatc cgtccgccgg
    30241 gctgtctctt ttgcggccaa caacggtatt gaggcgttaa cgctgtatgc ctttagtagt
    30301 gaaaactgga accgaccagc gcaggaagtc agtgcgttaa tggaactgtt tgtgtgggcg
    30361 ctcgatagcg aagtaaaaag tctgcaccga cataacgtgc gtctgcgtat tattggcgat
    30421 accagtcgct ttaactcgcg tttgcaagaa tgtattcgta aatctgaagc gctaacagcc
    30481 gggaataccg gtctgacgct gaatattgcg gcgaactacg gtggacgttg ggatatagtc
    30541 cagggagtca ggcaactggc tgaaaaggtg cagcaaggaa acctgcaacc agatcagata
    30601 gatgaagaga tgctaaacca gcatgtctgt atgcatgaac tggcccctgt agatttagta
    30661 attaggactg ggggggagca tcgcattagt aactttttgc tttggcaaat tgcctatgcc
    30721 gaactttact ttatagatgt tctctggccc gatttcgatg aacaagactt tgaaggggcg
    30781 ttaaatgcct ttgctaatcg agagcgtcgt ttcggcggca ccgagcccgg tgatgaaaca
    30841 gcctgatggg gggcgctttt gctgaagtat cgcctgatat ctgcttttgt gttaataccc
    30901 gtcgtcatcg cggcgttgtt tctgttgccg ccggtggggt tcgccattgt aacgctggtg
    30961 gtctgcatgc tggcagcgtg ggaatgggga cagcttagcg gttttaccac tcgttcgcag
    31021 cgagtatggt tggcggtgtt atgcgggtta ttgttggcgc tgatgctttt tctgttgccg
    31081 gaatatcacc gaaatattca tcaaccgctg gttgaaatct cactttgggc ttcgctgggt
    31141 tggtggattg tctcgctatt gctggtgctg ttttacccag gttccgcagc aatctggcgt
    31201 aactctaaaa cattgcgcct tatttttggc gtgctaacca ttgttccctt cttctggggc
    31261 atgctggcgt tacgggcctg gcactatgac gagaatcatt acagtggcgc aatatggctg
    31321 ctctatgtca tgatcctggt atggggcgct gactccggcg catatatgtt tggcaaattg
    31381 tttggtaaac ataagctggc accgaaggtt tctccgggta aaacctggca aggctttata
    31441 ggtggactcg ctactgcagc ggtaatctca tggggttatg gcatgtgggc gaatctcgac
    31501 gtcgctcccg tcaccttact catttgctct attgtcgcag cgttagcctc agtgctcggc
    31561 gatctgaccg agagtatgtt taagcgtgaa gcaggaatta aggacagcgg tcatttaatt
    31621 ccaggacacg gtggtatttt agatcgtatt gatagcctga cggctgcggt accggtcttt
    31681 gcttgcttgt tgttactggt attcaggacg ctttaacgga aggtaatatg ctgagttttc
    31741 tctgggattt ggcttcgttc atcgttgcac tgggtgtact tatcactgtg catgaatttg
    31801 gtcatttctg ggttgcccgg cgttgtggtg ttcgcgttga gcgtttctca atagggtttg
    31861 gtaaggcgct ctggcggcga actgataagc tcggcaccga atatgttatc gccctgatcc
    31921 cgctgggcgg ttatgtcaaa atgctggatg agcgcgcaga accggtcgtt ccggaactcc
    31981 gccaccatgc cttcaataat aaatctgttg gccaacgagc ggcgattatt gccgcaggtc
    32041 cggttgcaaa cttcattttt gctatctttg cctactggct ggtttttatt attggtgtgc
    32101 ctggcgtacg tccggtggtt ggtgaaatag cagccaattc gatagctgcg gaagcacaaa
    32161 ttgcaccagg tacggaacta aaagccgtag atggtatcga aacgcctgat tgggatgccg
    32221 tgcgtttgca gttggtcgat aaaattggcg atgaaagcac caccattaca gtagcgccat
    32281 ttggcagcga ccaacggcgg gatgtaaagc tcgatttacg tcactgggcg tttgagcctg
    32341 ataaagaaga tccggtatct tcgctgggga ttcgtcctcg tgggctgcaa attgaacctg
    32401 tactggaaaa tgtgcagcca aactcggcgg caagcaaggc aggtttgcaa gcaggcgaca
    32461 ggatcgttaa agtcgatggt cagcccttaa cgcagtgggt gacctttgtg atgcttgtcc
    32521 gggataaccc ggggaaatcc ttagcgttag aaatcgaaag gcagggaagt ctcttgtctt
    32581 tgacattaat cccggagagt aaaccgggta atggtaaagc gattggtttt gtcggtattg
    32641 agccgaaagt cattcctttg ccagatgagt ataaagttgt acgccagtat gggccgttca
    32701 acgccatcgt cgaagccacg gacaaaacgt ggcagctgat gaagctgacg gtcagtatgc
    32761 tgggaaaatt gatcaccggt gatgtgaaac tgaacaacct cagtgggccg atctctatcg
    32821 ccaagggggc tgggatgaca gcggaactcg gggtagttta ttacctgccg tttcttgcgc
    32881 ttattagcgt gaacttaggg ataattaacc tgtttccgtt gcccgtactt gacggggcgc
    32941 atctgctgtt ccttgcgatc gaaaagatca agggcggacc ggtatccgag cgggttcaag
    33001 acttttgtta tcgcattggc tcgattctgc tggtgctgtt aatggggctt gcacttttca
    33061 atgatttctc tcggttatga gagttagtta ggaagaacgc ataataacga tggcgatgaa
    33121 aaagttgctc atagcgtcgc tgctgtttag cagcgccacc gtatacggtg ctgaagggtt
    33181 cgtagtgaaa gatattcatt tcgaaggcct tcagcgtgtc gccgttggtg cggccctcct
    33241 cagtatgccg gtgcgtacag gcgacacggt taatgatgaa gatatcagta ataccattcg
    33301 cgctctgttt gctaccggca actttgagga tgttcgcgtc cttcgtgatg gtgataccct
    33361 tctggttcag gtaaaagaac gtccgaccat tgccagcatt actttctccg gtaacaaatc
    33421 ggtgaaagat gacatgctga agcaaaacct cgaggcttct ggtgtgcgtg tgggcgaatc
    33481 cctcgatcgc accaccattg ccgatatcga gaaaggtctg gaagacttct actacagcgt
    33541 cggtaaatat agcgccagcg taaaagctgt cgtgaccccg ctgccgcgca accgtgttga
    33601 cctaaaactg gtgttccagg aaggtgtgtc agctgaaatc cagcaaatta acattgttgg
    33661 taaccatgct ttcaccaccg acgaactgat ctctcatttc caactgcgtg acgaagtgcc
    33721 gtggtggaac gtggtaggcg atcgtaaata ccagaaacag aaactggcgg gcgaccttga
    33781 aaccctgcgc agctactatc tggatcgcgg ttatgcccgt ttcaacatcg actctaccca
    33841 ggtcagtctg acgccagata aaaaaggtat ttacgtcacg gtgaacatta ccgaaggcga
    33901 tcagtacaag ctttctggcg ttgaagtgag cggcaacctt gccgggcact ccgctgaaat
    33961 tgagcagctg actaagatcg agccgggcga gctgtataac ggcaccaaag tgaccaagat
    34021 ggaagatgac atcaaaaagc ttctcggtcg ctatggttat gcctatccgc gcgtacagtc
    34081 gatgcctgaa attaacgatg ccgacaaaac cgttaaatta cgcgtgaacg ttgatgcggg
    34141 taaccgtttc tacgtgcgta agatccgttt tgaaggtaac gatacctcga aagatgccgt
    34201 cctgcgtcgc gaaatgcgtc agatggaagg tgcatggctg ggaagcgatc tggtcgatca
    34261 gggtaaggag cgtctgaatc gtctgggctt ctttgaaact gtcgataccg atacccaacg
    34321 tgttccgggt agcccggacc aagtagatgt agtctacaag gtgaaagaac gtaacaccgg
    34381 tagcttcaac ttcgggattg gttacggtac tgaaagtggc gtgagcttcc aggctggcgt
    34441 gcaacaggat aactggttag gtacaggtta tgctgttggt atcaacggga ccaaaaacga
    34501 ttaccagacc tatgctgaat tgtcggtaac caacccgtac ttcactgtag atggtgtaag
    34561 cctcggtggt cgtctcttct ataatgactt ccaggcagat gacgccgacc tgtccgacta
    34621 taccaacaag agttatggta cagacgtgac gttgggcttc ccgatcaacg aatataactc
    34681 gctgcgtgca ggtctgggtt atgtacataa ctccctgtcc aacatgcagc ctcaggttgc
    34741 gatgtggcgt tatctgtact ctatgggtga acatccgagc acctctgatc aggataacag
    34801 cttcaaaacg gacgacttca cgttcaacta tggttggacc tataacaagc ttgaccgtgg
    34861 ttacttcccg acagatggtt cacgtgtcaa cctgaccggt aaagtgacca ttcctggatc
    34921 ggataacgaa tactacaaag tgacgttaga cacggcgact tatgtgccga tcgatgacga
    34981 tcacaaatgg gttgttctgg ggcgtacccg ctggggttat ggtgatggtt taggcggcaa
    35041 agagatgccg ttctacgaga acttctatgc cggtggttcc agcaccgtcc gtggcttcca
    35101 gtccaatacc attggtccga aagcagttta cttcccgcat caggccagta attatgatcc
    35161 ggactatgat tacgaatgtg cgactcagga cggcgcgaaa gacctgtgta aatcggatga
    35221 tgctgtaggc ggtaacgcca tggcggttgc cagcctcgag ttcatcaccc cgacgccgtt
    35281 tattagcgat aagtatgcta actcggttcg tacttccttc ttctgggata tgggtaccgt
    35341 ttgggataca aactgggatt ccagccaata ttctggatat ccggactata gtgatccaag
    35401 caatatccgt atgtctgcgg gtatcgcatt acaatggatg tccccattgg ggccgttggt
    35461 gttctcctac gcccagccgt tcaaaaagta cgatggagac aaggcagaac agttccagtt
    35521 taacatcggt aaaacctggt aagtgttctc cacaaaggaa tgtagtggta gtgtagcgat
    35581 gactttaggc gatcaatata agatcgccgg gccacgcaaa gaactgcacc ctccggtgca
    35641 aatgggatgg taaggagttt attgtgaaaa agtggttatt agcggcaggt ctcggtttag
    35701 cactggcaac ttctgctcag gcggctgaca aaattgcaat cgtcaatatg ggcagcctgt
    35761 tccagcaggt agcgcagaaa accggtgttt ctaacacgct ggaaaatgag ttcaaaggcc
    35821 gtgccagcga actgcagcgt atggaaaccg atctgcaggc taaaatgaaa aagctgcagt
    35881 ccatgaaagc gggcagcgat cgcactaagc tggaaaaaga cgtgatggct cagcgccaga
    35941 cttttgctca gaaagcgcag gcttttgagc aggatcgcgc acgtcgttcc aacgaagaac
    36001 gcggcaaact ggttactcgt atccagactg ctgtgaaatc cgttgccaac agccaggata
    36061 tcgatctggt tgttgatgca aacgccgttg cttacaacag cagcgatgta aaagacatca
    36121 ctgccgacgt actgaaacag gttaaataag taatgccttc aattcgactg gctgatttag
    36181 cgcagcagtt ggatgcagaa ctacacggtg atggcgatat cgtcatcacc ggcgttgcgt
    36241 ccatgcaatc tgcacaaaca ggtcacatta cgttcatggt taacccaaaa taccgtgagc
    36301 atttaggctt gtgccaggcg tccgcggttg tcatgaccca ggacgatctt cctttcgcga
    36361 aaagtgccgc gctggtagtg aagaatccct acctgactta cgcgcgcatg gcgcaaattt
    36421 tagataccac gccgcagccc gcgcagaaca ttgcacccag tgcggtgatc gacgcgacgg
    36481 cgaagctggg gaacaatgta tcgattggcg ctaacgcggt gattgaatcc ggcgttgaac
    36541 tgggcgataa cgtgattatc ggtgccggtt gcttcgtagg taaaaacagc aaaatcggtg
    36601 caggttcgcg tctctgggcg aacgtaacca tttaccatga gatccagatc ggtcagaatt
    36661 gcctgatcca gtccggaaca gtggtaggcg cagacggctt tggttatgcc aacgatcgtg
    36721 gtaactgggt taagatccca cagattggtc gcgtaattat tggcgatcgc gtggagatcg
    36781 gtgcctgcac taccatcgat cgcggcgcac tggatgacac tgttattggc aatggcgtga
    36841 tcattgataa ccagtgccag attgcacata acgtcgtgat tggcgacaat acggcggttg
    36901 ccggtggcgt cattatggcg ggcagcctga aaattggtcg ttactgcatg atcggcggag
    36961 ccagcgtaat caacgggcat atggaaatat gcgacaaagt gacggttacg ggcatgggta
    37021 tggtgatgcg tcccatcact gaaccaggcg tctattcctc aggcattccg ctgcaaccca
    37081 acaaagtctg gcgcaaaacc gctgcactgg tgatgaacat tgatgacatg agcaagcgtc
    37141 tgaaatcgct tgagcgcaag gttaatcaac aagactaacg ttccatcttt tgttcgccaa
    37201 actttacggc ctgtctcatt cttacgattg cggcaggccg tgttattatt gtcgtttctt
    37261 atattttgac aggaagagta tcttgactac taacactcat actctgcaga ttgaagagat
    37321 tttagaactt ctgccgcacc gtttcccgtt cttactggtg gatcgcgtgc tggattttga
    37381 agaaggtcgt tttctgcgcg cagtaaaaaa tgtctctgtc aatgagccat tcttccaggg
    37441 ccatttccct ggaaaaccga ttttcccggg tgtgctgatt ctggaagcaa tggcacaggc
    37501 aacaggtatt ctggcgttta aaagcgtagg aaaactggaa ccgggtgagc tgtactactt
    37561 cgctggtatt gacgaagcgc gcttcaagcg cccggtcgtg cctggcgatc aaatgatcat
    37621 ggaagtcact ttcgaaaaaa cgcgccgcgg cctgacccgt tttaaagggg ttgctctggt
    37681 cgatggtaaa gtagtttgcg aagcaacgat gatgtgtgct cgtagccggg aggcctgata
    37741 cgtgattgat aaatccgcct ttgtgcatcc aaccgccatt gtggaagagg gcgcgtcaat
    37801 tggcgcgaac gcacacattg gtcctttttg tatcgttgga ccccatgtcg gaattggtga
    37861 gggtaccgta ctgaaatctc acgttgtcgt gaatggtcat actaaaattg gccgcgataa
    37921 tgagatttat cagttcgcct ccatcggcga agttaaccag gatctgaaat atgctggcga
    37981 accgacccgt gtggaaatcg gcgatcgtaa ccgcattcgc gaaagcgtca ccattcatcg
    38041 tggcacagtc cagggcggtg gattgacgaa ggtgggcagc gacaacttac tgatgatcaa
    38101 cgcgcacatt gcgcacgatt gtacggtagg taaccgctgt attctcgcca acaacgcaac
    38161 gctggcgggt cacgtatcgg ttgacgactt cgcgatcatc ggcggcatga ccgcagtcca
    38221 tcagttctgc atcattggtg cgcacgtgat ggttggcggc tgctccggtg tggcgcagga
    38281 cgtccctcct tatgtcattg cgcagggtaa ccacgcaacg ccgttcggtg tcaatatcga
    38341 agggctgaag cgccgcggat tcagccgtga ggcgattacc gctatccgca atgcgtataa
    38401 gctgatttat cgtagcggta aaacgctcga tgaagtgaaa ccggaaattg ctgaactggc
    38461 ggaaacatat ccggaagtga aagcctttac cgatttcttt tcacgctcaa cgcgcggtct
    38521 gattcgttaa tgactgaaca gcgtccatta acgattgccc tggtcgccgg agaaacctcc
    38581 ggcgatatcc taggggccgg tttaatccgc gctctgaaag aacgtgtgcc caacgcccgc
    38641 tttgttggtg ttgccgggcc acgaatgcag gctgaaggct gcgaagcctg gtacgaaatg
    38701 gaagaactgg cggtgatggg cattgttgaa gtgctcggtc gtctgcgtcg cttactgcat
    38761 attcgtgccg atctgacaaa gcgttttggc gaactgaagc cagatgtttt tgttggtatt
    38821 gatgcgcctg acttcaatat tactcttgaa ggtaacctca aaaagcaggg tatcaaaacc
    38881 attcattacg tcagtccgtc agtctgggcg tggcgacaga aacgtgtttt caaaataggc
    38941 agagccaccg atctggtgct cgcatttctg cctttcgaaa aagcgtttta tgacaaatac
    39001 aacgtaccgt gccgctttat cggtcatacc atggctgatg ccatgccatt agatccagat
    39061 aaaaatgccg cccgtgatgt gctggggatc cctcacgatg cccactgcct ggcgttgcta
    39121 ccggggagcc gtggtgcaga agttgaaatg cttagtgccg atttcctgaa aacggcccag
    39181 cttttgcgcc agacatatcc ggatctcgaa atcgtggtgc cactggtgaa tgccaaacgc
    39241 cgcgagcagt ttgaacgcat caaagctgaa gtcgcgccag acttttcagt tcatttgctg
    39301 gatgggatgg gccgtgaggc gatggtcgcc agcgatgcgg cgctactggc gtcgggtacg
    39361 gcagccctgg agtgtatgct ggtgaaatgc ccgatggtgg tgggatatcg catgaagcct
    39421 tttaccttct ggttggcgaa gcggctggtg aaaactgatt atgtctcgct gccaaatctg
    39481 ctggcgggca gagagttagt caaagagtta ttgcaggaag agtgtgagcc gcaaaaactg
    39541 gctgcggcgc tgttaccgct gttggcgaac gggaaaatca gccacgcgat gcacgatacc
    39601 ttccgtgaac tgcatcagca gatccgttgc aatgccgatg agcaggcggc acaagccgtt
    39661 ctggagttag cacaatgatc gaatttgttt atccgcacac gcagctggtt gcgggtgtgg
    39721 atgaagtcgg acgcgggccg ttagttggcg cggtcgtcac cgctgcggtg atccttgacc
    39781 cggcgcgccc gattgccggg ctgaatgatt ccaaaaagct gagcgaaaaa cgccgtctgg
    39841 cgctctgtga agagatcaaa gagaaagcgt tgagctggag tctgggccgc gcggaacccc
    39901 acgaaatcga cgagctgaac attcttcatg cgaccatgct ggcgatgcag cgtgccgtcg
    39961 ctgggctgca tattgcgccg gaatatgtgt tgattgatgg taaccgctgc ccgaaattac
    40021 cgatgcctgc gatggttgtg gtgaaaggcg atagccgcgt accggaaatc agtgccgcgt
    40081 ctatcctggc gaaagtgacg cgtgacgccg aaatggcggc gctggatatt gttttcccgc
    40141 aatatggttt tgcccaacac aaagggtacc caaccgcttt tcatctggaa aaactggctg
    40201 aacacggcgc gaccgaacac catcggcgca gctttgggcc tgtcaaacgc gcactgggac
    40261 ttgcgtcctg attcttgtgt cgagattaag taaaccggaa tctgaagatg tctgaaccac
    40321 gtttcgtaca cctgcgggtg cacagcgact actcgatgat cgatggcctg gccaaaactg
    40381 caccgttggt aaaaaaggcg gcggcgttgg gtatgccagc actggcgatc accgatttca
    40441 ccaacctttg tggtctggtg aagttctacg gagcgggaca tggcgcaggg attaagccta
    40501 tcgtcggggc agattttaac gtccagtgcg acctgctggg tgatgagtta acccacctga
    40561 cggtactggc ggcgaacaat accggctatc agaatctgac gttgctgatc tcaaaagcgt
    40621 atcagcgcgg gtacggtgcc gctgggccga tcatcgatcg cgactggctt atcgaattaa
    40681 atgaagggtt gatccttctt tccggcgggc gcatgggcga cgtcggacgc agtcttttgc
    40741 gtggtaacag cgcgctggta gatgagtgtg tcgcgtttta tgaagaacac ttcccggatc
    40801 gctattttct cgagctgatc cgcaccggca ggccggatga agaaagctat ctgcacgcgg
    40861 cggtggaact ggcggaagcg cgcggtttgc ccgtcgtggc gactaacgat gtgcgcttta
    40921 tcgacagcag cgactttgac gcacacgaaa tccgcgtcgc gatccacgac ggctttaccc
    40981 tcgacgatcc taaacgcccg cgtaactatt cgccgcagca atatatgcgt agcgaagagg
    41041 agatgtgtga gctgtttgcc gacatccccg aagcccttgc caacaccgtt gagatcgcca
    41101 aacgctgtaa cgtaactgtg cgtcttggtg aatacttcct gccgcagttc ccgaccgggg
    41161 acatgagcac cgaagattat ctggtcaagc gtgcaaaaga gggcctggaa gagcgtctgg
    41221 cctttttatt ccctgacgag gaagaacgtg ttaagcgccg cccggaatat gacgaacgtc
    41281 tggagactga acttcaggtt atcaaccaga tgggcttccc gggctacttc ctcatcgtta
    41341 tggaatttat ccagtggtcg aaagataacg gcgtaccggt agggccaggc cgtggctccg
    41401 gtgcgggttc actggtggcc tacgcgctga aaatcaccga cctcgatccg ctggaatttg
    41461 acctgctgtt cgaacgtttc cttaacccgg aacgtgtctc catgcctgac ttcgacgttg
    41521 acttctgtat ggagaaacgc gatcaggtta tcgagcacgt agcggacatg tacggtcgtg
    41581 atgcggtatc gcagatcatc accttcggta caatggcggc gaaagcggtg atccgcgacg
    41641 taggccgcgt gctggggcat ccgtacggct ttgtcgatcg tatctcgaaa ctgatcccgc
    41701 ccgatccggg gatgacgctg gcgaaagcgt ttgaagccga gccgcagctg ccggaaatct
    41761 acgaagcgga tgaagaagtt aaggcgctga tcgacatggc gcgcaaactg gaaggggtca
    41821 cccgtaacgc cggtaagcac gccggtgggg tggttatcgc gccgaccaaa attaccgatt
    41881 ttgcgccgct ttactgcgat gaagagggca aacatccggt cacccagttt gataaaagcg
    41941 acgttgaata cgccggactg gtgaagttcg acttccttgg tttgcgtacg ctcaccatca
    42001 tcaactgggc gctggagatg atcaacaagc ggcgggcgaa gaatggcgag ccgccgctgg
    42061 atatcgccgc gatcccgctg gatgacaaga aaagcttcga catgctgcaa cgctcggaaa
    42121 ccacggcggt attccagctt gaatcgcgcg gcatgaagga tctgatcaag cgtctgcaac
    42181 ctgactgctt cgaagatatg atcgcactgg tggcgctgtt ccgcccaggt ccgttgcaat
    42241 cagggatggt ggataacttt atcgaccgta aacatggtcg cgaagaaatc tcctatccgg
    42301 acgtacagtg gcagcatgaa agcctgaaac cggtactgga gccaacctac ggcatcatcc
    42361 tgtatcagga acaggtcatg cagattgcgc aggtgctttc tggttatacc ctcggtggcg
    42421 cggatatgct gcgtcgtgcg atgggtaaga aaaagccgga agagatggct aagcagcgtt
    42481 ctgtatttgc tgaaggtgca gaaaagaacg gagtcaacgc cgaactggcg atgaaaatct
    42541 tcgacctggt ggagaaattc gcgggttacg gatttaacaa atcgcactct gcggcctatg
    42601 ctttggtgtc atatcaaacg ttatggctga aagcgcacta tccggcggag tttatggcgg
    42661 cggtaatgac cgccgatatg gacaacaccg agaaggtggt gggcctggtg gatgagtgct
    42721 ggcggatggg gctgaaaatc ctgccaccag atataaactc cggtctttac catttccacg
    42781 tcaacgacga cggcgaaatc gtgtatggta ttggcgccat caaaggggta ggtgaaggtc
    42841 cgattgaggc catcatcgaa gcccgtaata aaggcggcta cttccgcgaa ctgtttgatc
    42901 tctgcgcccg taccgacacc aaaaagttaa accggcgggt gctggaaaaa ctgatcatgt
    42961 ccggggcgtt tgaccgtctt gggccacacc gcgcggcgct gatgaactcg ctgggtgatg
    43021 cgttaaaagc ggcagatcaa cacgcgaaag cggaagctat cggtcaggcc gatatgttcg
    43081 gcgtgctggc cgaagagccg gaacaaattg aacaatccta cgccagctgc caaccgtggc
    43141 cggagcaggt ggtgttagat ggggaacgtg aaacgttagg tctgtacctt acgggacacc
    43201 ctatcaacca gtatttaaaa gagattgagc gttatgtcgg aggcgtaagg ctgaaagaca
    43261 tgcacccgac agaacgtggt aaagtcatca cggctgcggg gctcgttgtt gccgcgcggg
    43321 ttatggtcac caagcgcggc aatcgtatcg gtatctgcac gctggatgac cgttccgggc
    43381 ggctggaagt gatgttgttt actgacgccc tggataaata ccagcaattg ctggaaaaag
    43441 accgcatact tatcgtcagc ggacaggtca gctttgatga cttcagcggt gggcttaaaa
    43501 tgaccgctcg cgaagtgatg gatattgacg aagcccggga aaaatatgct agcgggcttg
    43561 ctatctcgct gacggacagg caaattgatg accagctttt aaaccgactc cgtcagtctc
    43621 tggaacccca ccgctctggg acaattccag tacatctcta ctatcagagg gcggatgcac
    43681 gcgcgcggtt gcgttttggc gcgacgtggc gtgtctctcc gagcgatcgt ttattaaacg
    43741 atctccgtgg cctcattggt tcggagcagg tggaactgga gtttgactaa tacaggaata
    43801 ctatgagtct gaatttcctt gattttgaac agccgattgc agagctggaa gcgaaaatcg
    43861 attctctgac tgcggttagc cgtcaggatg agaaactgga tattaacatc gatgaagaag
    43921 tgcatcgtct gcgtgaaaaa agcgtagaac tgacacgtaa aatcttcgcc gatctcggtg
    43981 catggcagat tgcgcaactg gcacgccatc cacagcgtcc ttataccctg gattacgttc
    44041 gcctggcatt tgatgaattt gacgaactgg ctggcgaccg cgcgtatgca gacgataaag
    44101 ctatcgtcgg tggtatcgcc cgtcttgacg gtcgtccggt gatgatcatt ggtcatcaaa
    44161 aaggtcgtga aaccaaagaa aaaattcgcc gtaactttgg tatgccagcg ccagaaggtt
    44221 accgcaaagc actgcgtctg atgcaaatgg ctgaacgctt taagatgcct atcatcacct
    44281 ttatcgacac cccgggggct tatcctggcg tgggcgcaga agagcgtggt cagtctgaag
    44341 ccattgcacg caacctgcgt gaaatgtctc gcctcggcgt accggtagtt tgtacggtta
    44401 tcggtgaagg tggttctggc ggtgcgctgg cgattggcgt gggcgataaa gtgaatatgc
    44461 tgcaatacag ctcctattcc gttatctcgc cggaaggttg tgcgtccatt ctgtggaaga
    44521 gcgccgacaa agcgccgctg gcggctgaag cgatgggtat catcgctccg cgtctgaaag
    44581 agctgaaact gatcgactcc atcatcccgg aaccgctggg cggcgctcac cgtaacccgg
    44641 aagcgatggc ggcatcgttg aaagcgcaac tgctggcgga tctggccgat ctcgatgtgt
    44701 taagcactga agatttaaaa aatcgtcgtt atcagcgcct gatgagctac ggttacgcgt
    44761 aattcgcaaa agttctgaaa aagggtcact tcggtggccc ttttttatcg ccacggtttg
    44821 agcaggctat gattaaggaa ggattttcca ggaggaacac atgaacatca ttgccattat
    44881 gggaccgcat ggcgtctttt ataaagatga gcccatcaaa gaactggagt cggcgctggt
    44941 ggcgcaaggc tttcagatta tctggccaca aaacagtgtt gatttgctga aatttatcga
    45001 acataaccct cgaatttgcg gcgtgatttt tgactgggat gagtacagtc tcgatttatg
    45061 cagcgatatc aatcaactta atgaatatct cccgctttat gccttcatca acacccactc
    45121 gacgatggat gtcagcgtgc aggatatgcg gatggcgctc tggttttttg aatatgcgct
    45181 ggggcaggcg gaagatatcg ccattcgtat gcgtcagtac accaacgaat atcttgataa
    45241 cattacgccg ccgttcacga aagccttgtt tacctacgtt aaagagcgga agtacacctt
    45301 ttgtacgccg gggcatatgg gcggcaccgc atatcaaaaa agcccggttg gctgcctgtt
    45361 ttatgatttt ttcggcggga ataccctcaa ggctgatgtc tctatttcgg tcaccgagct
    45421 tggttcgttg ctcgaccaca ccgggccaca cctggaagcg gaagagtaca tcgcgcggac
    45481 ttttggcgcg gaacagagtt atatcgttac caacggaaca tcgacgtcga acaaaattgt
    45541 gggtatgtac gccgcgccat ccggcagtac gctgttgatc gaccgcaatt gtcataaatc
    45601 gctggcgcat ctgttgatga tgaacgatgt agtgccagtc tggctgaaac cgacgcgtaa
    45661 tgcgttgggg attcttggtg ggatcccgca ccgtgaattt actcgcgaca gcatcgaaga
    45721 gaaagtcgct gccaccacgc aggcacaatg gccggttcat gcggtgatta ccaactccac
    45781 ctatgatggc ttgctctaca acaccgactg gatcaaacag acgctggatg tcccgtcgat
    45841 ccacttcgat tccgcctggg tgccgtacac ccattttcat ccgatctacc aggggaaaag
    45901 tgatcttcga aacgcaatcg acccacaaaa tgctggcggc gttatcgcag gcttcgctga
    45961 tccacattaa aggcgagtat gacgaagagg cgtttaacga agcctttatg atgcatacca
    46021 ccacctcgcc cagttatccc attgttgctt cggttgagac ggcggcggcg atgctgcgtg
    46081 gtaatccggg caaacggctg attaaccgtt cggtggagcg ggcgctgcat tttcgcaaag
    46141 aggtccagcg gttgcgggaa gagtctgacg gttggttttt cgatatctgg caaccgccgc
    46201 aggtggatga agccgaatgc tggcccgttg cgccaggcga acagtggcac ggctttaacg
    46261 atgcggatgc cgatcatatg tttctcgatc cggttaaagt cactattttg ataccgggga
    46321 tggacgaaca gggcaatatg agcgaggagg ggatcccggc ggcgctggtg gcaaaattcc
    46381 tcgacgaacg tgggatcgta gtagagaaaa ccggccctta taacctgctg tttctcttta
    46441 gtattggtat cgataaaacc aaagcaatgg gattattgcg tgggttgacg gaatttaaac
    46501 gctcttacga tctcaacctg cggatcaaaa atatgctacc cgatctctat gctgaagatc
    46561 ccgatttcta ccgcaatatg cgtattcagg atctggcgca agggatccat aagctgattc
    46621 gtaaacacga tcttcccggt ttgatgttgt gggcattcga tactttgccg gaaattatca
    46681 tgacgccaca tcaggcatgg cagagacaga ttaaaggtga agtagaaacc attgcgctgg
    46741 aacaactggt cggtagagta tcggcaaata tgagcctgcc ttatccaccg ggcgtaccgc
    46801 tgttgatgcc aggagaaatg ctgaccaaag agagccgcac ggtactcgat tttctactga
    46861 tgctttgttc cgtagggcaa cattaccccg gttttgaaac ggatattcac ggcgcgaaac
    46921 aggacgaaga cggcgtttac cgcgtacgag tcctaaaaat ggcgggataa cttgccagag
    46981 cggcttacgg gcgagtaacg tgctgttaac aaataaagga gacgttatgc tgggtttaaa
    47041 acaggttcac catattgcga ttattgcgac ggattatgcg gtgagcaaag ctttctactg
    47101 cgatattctt ggtttcacgc tgcaaagcga agtctatcgc gaagcgcgcg actcgtggaa
    47161 aggggatttg gcgcttaatg ggcaatatgt gattgagctt ttctcttttc cgttcccgcc
    47221 ggagcgcccc agccgaccgg aagcttgcgg cctgcgtcat ctggctttta gcgttgatga
    47281 catcgatgcg gcagtggcgc accttgaaag ccataacgtg aagtgtgaag ccatccgtgt
    47341 cgatccatac acgcaaaaac gcttcacctt ctttaacgat ccggacgggc tgccgttgga
    47401 actgtatgag cagtaaggct tgtcatcgcc gcatttgccc ggtaacgtgc cgggcattgc
    47461 tactgtaaaa tcgcaccatc atgacactca cgctcaatag acaacttctc acctcacgcc
    47521 agattttggt cgcctttagc ggcgggcttg actccaccgt tctgctgcat cagttggtgc
    47581 agtggcggac ggaaaatccg ggcgtcactc tgcgcgctat tcatgtgcat cacggtttaa
    47641 gtgccaatgc cgatgcctgg gttacgcatt gcgaaaacgt ctgccaacag tggcaggtgc
    47701 cgctggtggt cgaacgcgta caacttgcgc aagaggggct ggggattgag gcccaggcgc
    47761 ggcaggcgcg ttatcaggca tttgcccgta ccctattgcc cggcgaagtg ctggtcaccg
    47821 cgcaacatct cgacgatcaa tgtgaaacct ttctgctggc gctaaaacgc ggtagcggcc
    47881 ctgccgggct ttcggctatg gcggaagttt cggagtttgc cggaacacgg cttatccgcc
    47941 cgttgcttgc ccgcacgcgg ggggaactgg cgcagtgggc attggcgcat ggtttacgct
    48001 ggattgaaga cgaaagtaat caggacgact catacgatcg taactttctg cgcctgcgcg
    48061 tagtgccgtt attgcagcag cgttggccgc attttgcgga agcaacggcc cgcagcgccg
    48121 cactttgtgc tgaacaagag agtctgttgg atgaactgct ggcggatgat ttagcacact
    48181 gccaaacgtc gcaggggacg ctgcagattg cgccaatgct ggcgatgagt gatgcccgcc
    48241 gcgcggcgat tatccgtcgc tggctggcag ggcagaatgc accaatgcct tcccgcgaag
    48301 cgctggtgcg gatctggcag gaagtggcgc tggcgcggga agatgcctcc ccctgtttac
    48361 gcttgggcgc gtttgaaatc cgccgctatc agtcgcaact gtggtggatt aaatccgtca
    48421 ccgggcaaag tgaaaccatt gtgccgtggc agacgtggct acaaccgctg gaattaccgg
    48481 cggggctggg aagcgtacag cttactgcgg gaggcgatat tcgccctccg cgtgcagacg
    48541 aagcggtcag cgtgcgtttc aaatcggcag gattgctgca tattgtcggg cgcaacggcg
    48601 gacgtaagct gaagaaaatc tggcaagagt taggcgtgcc gccgtggcta cgtgacacca
    48661 cgccactgct gttttatggc gaaacgctga ttgcggcggc aggggtattt gtgacgcaag
    48721 aaggtgtggc tgaaggtgag aatggcgtaa gttttgtctg gcagaaaacg cttagttaag
    48781 tgaaagccgg ataagacgca tcaaacgtcg catccggcga aagtcaatca ggactcgctc
    48841 accaccaccg taccgatttc cgggtggcta aagctggtaa ttttatccag acgtagctca
    48901 cgggtttcgc cagcggcctc gacgaccaga tactccacat ttttgcggga gactaaatcg
    48961 ctggcttttg cctgcaattt ttcgccatct ttcagctcaa gtgtcagcat taaatgatgc
    49021 tggcaggcga gctcaagatt atcgtaatca tcacaattga ttggttgata cgtatcattc
    49081 attaacataa tcgctcacca gtaagtttgc cgcagcgtat gctgcttttt ccctgacagc
    49141 ctcagaaagg gcgtcgtcgg cagccatttc attcagcact ttcaaaacgc agcccagcgc
    49201 gtccggaacg tatcctaagt ctccgctggc gatttccgcg taccgcttgc gtattaactc
    49261 acaatatttt tccacatgcc ctcctgtcag cacactgact taaccgtgga tgcaagtcta
    49321 agcctacgaa gataaactct gtttcgcaag gtgactatac cacactcatt tctgcaatat
    49381 cagcgccgca actgcacgta ttccgttaca atggcctcct gattcgaaag gagttttctt
    49441 atggcgctta aagcgacaat ttataaagcg acggttaatg tggccgatct cgaccgcaac
    49501 cagtttctcg atgcctctct gacgctggcg cgccaccctt cagaaaccca ggagcgtatg
    49561 atgctgcgcc tgctggcgtg gctgaaatat gctgatgaac gtctgcaatt tacccgtggt
    49621 ttgtgtgccg atgatgagcc ggaagcgtgg ctgcgtaacg atcacctggg cattgatttg
    49681 tggattgagc tggggctgcc ggatgagcgg cggattaaga aagcctgcac ccaggccgca
    49741 gaagtggcgc tgtttaccta taatagtcgg gcggcgcaaa tctggtggca gcaaaatcag
    49801 agcaaatgtg tgcagtttgc caatctttcc gtctggtatc tggacgatga acaactggcg
    49861 aaagtaagcg cctttgccga tcgtaccatg acgctgcagg caacgattca ggatggcgtg
    49921 atctggttat cggacgataa gaataatctg gaagttaact taaccgcctg gcaacaacct
    49981 tcatgattgt gatttcccga catgttgcta ttcccgatgg tgagcttgag atcaccgcca
    50041 ttcgtgcgca gggcgcgggc gggcagcatg ttaataagac ctcaacggcc attcatctgc
    50101 gttttgacat tcgggcgtcc agcctgccag agtattacaa agagcgtctg ctcgccgcca
    50161 gccatcattt gatcagcagt gatggcgtga ttgtcattaa ggcacaggaa taccgcagtc
    50221 aggaactgaa ccgcgaagcc gcgctagccc ggctggtggc agtgattaaa gatttaacaa
    50281 cagaacaaaa agcccgacga cccacgcggc ccacccgtgc atcgaaagag cgcaggctgg
    50341 catcgaaagc acaaaaatca agcgcgaagg cgatgcgcgg caaagtacgc agcggtcggg
    50401 aataaaaaga aggaatagat ggtgaaaaaa gcgatagtga cagcgatggc tgtaatcagc
    50461 ctctttactc tgatgggatg taataaccgg gccgaagtcg atacgctttc tccagcacag
    50521 gctgccgaac tgaaaccgat gccgcaaagt tggcgcggcg tgctgccgtg tgctgattgt
    50581 gaaggaatcg aaacctctct gttcctcgaa aaagacggaa catgggtgat gaatgagcgt
    50641 tatctcggtg ctcgtgaaga accttcctcc ttcgcttcct acggtacatg ggcgcgaacc
    50701 gctgacaagc tggtattaac cgacagcaaa ggtgaaaagt catattatcg ggcgaaaggc
    50761 gatgcgctgg agatgctcga tcgtgaaggc aatccgattg aatcgcagtt caactatacg
    50821 ctggaaccgg cacaatccag tttacctatg acgccgatga ccctgcgagg catgtatttt
    50881 tatatggctg atgcggcgac cttcactgat tgcgcgaccg gaaaacgttt catggtagcg
    50941 aataacgcag agctggagcg tggctacctg gctgcgcgcg gtcacagtga aaaaccggtg
    51001 ttactgtcag tagaaggtca ctttacgctt gaggctaatc cggatactgg tgcgccaacc
    51061 aaagtattag cgcccgatac ggcgggtaaa ttttacccga ataaggattg cagtagtttg
    51121 gggcagtaaa cctgcttaag gcagaaacaa acgcaaaaca ggccagaagg atatatttca
    51181 acattttgaa tttgcacgtt ttttgtaggc cggataaggc gtttacgccg catccggcaa
    51241 tggtgctcaa agcctgatgc gacgctggcg cgtcttatca tgcctacaac ccccctcata
    51301 ccttaaggcg gataaggcat ttactttatc accgggtcaa accaacaaag cgacccgcct
    51361 tgatataaat ccctggcttc aggtgcccaa catactgtaa ctgcgtttct ggcttaaacg
    51421 cagagacatc accaatacgc atatgcatca gatcggcagg gctaatccag cccgattgcg
    51481 ccagtgtaag tggatgtccg gctttggcaa atgcatcagt gacaaattcc gaacaaaacc
    51541 acgacttttt gtctccttcg cccacactgc ttaactgcgc tttcgccagg ccgctgacgc
    51601 actgctggcg aaaatcctcg gagaactgat tcagtgagca catctggcga gtcaccataa
    51661 agggaataaa ttcgacaatg ccgcgatagt tataaccgct atctttgatt ttattggcaa
    51721 acgcggtgat atctgtggct tgttgcgggg taagatccgg gactcgtaag acgaaaagct
    51781 tatcactatg cttcatcgct tttttaaggg aaacaatctg gacgccagcg cctgtcgctt
    51841 ctgcaacgtt attatcaccc agaaagattg caacgtgact cacagaggaa gtgctgaaga
    51901 cgcggattcc aaatgaggtt accccaaggc ttgaggagaa cagcaaatcg ccgggtttga
    51961 gatcaggtgc cgttatttct tttattgatt gttcggtgaa agagctttga tgctggaatt
    52021 tgacagccca tgtttttgcc tcggcatcta ctgctgtggc tgatggggct ggctgatatc
    52081 aacggtacaa gccgaaagta aaagaaagct ggggagaagc aggcggcagt acgcctttgg
    52141 tttatccatt ttatacaatc catgtaaaaa agggccctga aattcaggac cctttctggc
    52201 atcagccttt aatctgtttc accagatatt cgacgatgtc accagtctta attaactgtt
    52261 tctcgccgtt gcgacgatat ttatattcga tatcgtcgtt gtcgaggtta cggtcgccca
    52321 gcacaatagt gtgcggaata ccgatcagtt ccatatcagc aaacatcacg cccggacgct
    52381 ctttgcggtc atccagcagc acttcgatac cttgtgcacg cagttcgctg tacagtttct
    52441 cagcaagctc ttgtacgcgg aaggatttgt gcatgttcat cggcagaatc gccacctgga
    52501 acggcgcgat agcgtcaggc catacgatgc cgcgttcgtc gtagttctgc tcaatcgccg
    52561 cagctaccac acgcgttacc ccgataccgt agcaacccat cgtcaggatt tggttacggc
    52621 catcttcacc ctgtacggag gctttcagtg cttcggagta cttggtaccc agctggaaga
    52681 tgtgaccaac ttcgatacca cgtttgatca gcagcgtacc ctggccatcc gggcttggat
    52741 cgccagccac cacgttacgg atatctgcaa cttccggggt agcgacatcg cgatcccagt
    52801 tgataccgaa gtagtgttta ccatcgatgt tagcaccagc agcgaaatca ctcatcgccg
    52861 caacggtacg gtcaatcacc accggaatcg gcatgtttac cggacccagt gaacccggac
    52921 cggctttaac cacagcacga atttcttctt cggtcgcgaa agtcagcggg cttgcaacct
    52981 gcggcagttt ttctgcttta acttcgttca gctcgtgatc accgcgcacc agcagcgcaa
    53041 ccagcgggaa gctgctgcct tcaaccgctt taaccagcag agtcttaacc gttttctcaa
    53101 tcggcagatt gaactgttca accagttccg cgatggtttt cgcgttcggc gtatcaacca
    53161 gcgtcatttc ctgggtagca gcagcgcgcg gttctttcgg cgcgatagct tctgccagtt
    53221 caatgttcgc tgcatagtca gaggtgtcgg agaagaccac atcgtcttca ccgctctgcg
    53281 ccagcacctg gaattcgtga gaggcgctgc cgccgataga accggtgtcg gcttgcacgg
    53341 cgcggaaatc cagccccatg cggctgaaga ttttgctgta ggccgcatac attgcatcgt
    53401 aggtttcctg cagggattcc tgagaagtat ggaaagagta agcatctttc atcaggaatt
    53461 cgcgggaacg catgacgccg aaacgcggac gcacttcgtc gcggaacttg gtctggatct
    53521 gatagaagtt caacggcagc tgtttgtaag agctaagctc gttacgaatc aggtcagtga
    53581 taacttcttc atgagttggg ccgagtacga acggacgctc gccacggtca acaaaacgca
    53641 gcagttccgg accgtactgt tcccaacgac cactctcttg ccacaaatcg gctggctgaa
    53701 ccaccggcat cgacacctcg attgcaccgg cgttgttcat ctcttcacgc acgatgtttt
    53761 cgactttttt cagaacgcgc acgccggtcg gcagccaggt atataacccg gaggccagct
    53821 tgcggatcat cccggcgcgc agcatcagct gatggctgat cacctcggcg tcggcaggtg
    53881 tctccttgag agtggagagc aggtattggc tagtacgcat gttgttacgg ttccagttgg
    53941 aaggtagaac aggctcaagg cgagcctggg acaaaaaagt gatttagttt accagtgcaa
    54001 aagaaatgtc aaaagagaag ggcgtgaatt taacgcggtt ccagcgcaaa gacttcaaaa
    54061 cctgcgtcgg tgacgcgcca gcgaacgtta aaatcatgta gccagacggc ataggttttg
    54121 cccgtttcct cacctttatg ataggccggg cgcgggtcct gcgccagtac ttcgcggata
    54181 aacagcgtta actgcggata acgcttctcc agcgtcaaaa gctgcttttc gacctctgcg
    54241 gtaaaactca ccgccatctc tgcagctggc gcactttgcg catagctggc actggcatcg
    54301 ggaagcgatt cggcaaaggg gagatacggt ttgatatcca ctaccggcgt accatcgacc
    54361 agatccagac tgccgagctt cagaatcacg ctgtctttat ggcaaacaac ctctttcagc
    54421 tctaccagcg acataccaat tgggttaggg cggaaagtag agcgtgttgc gaaaaccccc
    54481 attctggcgt taccgccgag gcgcggtgga cgcacagtcg gacgccagcc gccttccatc
    54541 gtttgatgaa agacgaaaag gatccataaa tggctgaacg cttccaggcc gcgaacggcg
    54601 tcggcctggt tgtagggagc aatgagatgc agttcgccgt tggcgctttt taccagacct
    54661 ggctggcgcg gaacggcgaa tttttcttta tagggcgagc gaataacgcc tatttgctcg
    54721 aactggaaac tgctcatttc gccgtaatgt taagcgcaga gccgatacat acagcctgac
    54781 gatagcagcc tggcgtaccg ctggtgactt cgcagctatg cagtaatacc gcattggctt
    54841 tcattttaga ggcgttgatt tgcatccgct tacgcgcggt tggaatgctc ggcggagagt
    54901 cctgattaga ggcctggcaa gagtcgccac tgacttcacc gagatcgcgg aacggtttgc
    54961 cgactaattc ttctgcattg gtataaattc ggaccggcgt ggcgcgcggc gctttcggtt
    55021 ttgcaggctc cgctttcggc tggggtgcag tgctttgaac gggttcgaca ggggatctgc
    55081 ttaacatgga acagccgctt agcatgagtg ctactaaaca gatcggtaaa gcacgcatag
    55141 tatttcctca atgtatgatc aaaacgtcaa tattgaatca ggagcttgta aaaatgacaa
    55201 gacgggcaag tgcccatcct gaatgatatt acaaattgtg gaaacagcct aaaaattacc
    55261 agcctttaac agcgccgccg ttaaacactt tgtttgctgc ttcgtaaact tcatcagact
    55321 gataagcctg gacgaatttc ttcacgttct cggcgtcttt gttatcttca cgcgttacga
    55381 tcaggtttac gtacggggag tctttatctt cgacaaagat accgtctttc gctggagtca
    55441 ggccaatctg gctggcatag gtggtattga taactgccag agcgatttgt gcgtcgtcca
    55501 gagatcgagg cagctgcggt gcttccagct caacaatttt cagatttttt gggttctcaa
    55561 caacatcaag tacggtcggc agcaggccaa cgccatcttt cagtttgatc aagcccactt
    55621 tttgcagcag cagcagtgaa cgaccaaggt tagttggatc gtttggcacg gcaacctgcg
    55681 aaccatcctg cagttcatcc agtgatttga ttttcttgga gtaaccagca atcggataaa
    55741 caaaagtgtt gcctactgcg accagtttgt agccacgatc tttcagttgc tgatcaaggt
    55801 acggtttatg ctggaaggcg ttggcgtcga tatcgccttt gctcaatgct tcgtttggca
    55861 gaacatagtc gttgaaggtt accagctcaa cgtccaggcc atatttgtct ttcgcaactt
    55921 tctgcgcgac ttctgcaacc tgctgttcgg caccaacaat cacgccgact ttaatgtggt
    55981 ttggatcttt ttcatcctga ccgcagccta ccagtgccag tgatccaatc agggctccca
    56041 ctgccgcaaa ggttttgaat ttgaacgcca taccttattc cttcttcaat tatttatgtt
    56101 gtgttgaacg ttacttgcga gtgacagccc ggacgatgcg gtcgcctgcg aactgaatta
    56161 aataaaccag aatgaccagc aataccagta ccgtattcat caccgtcgcg ttatagccga
    56221 tgtagccata ctgatagcca atctgaccta aaccaccggc accgacagca ccacccatcg
    56281 cggaataacc gactagggta atcagggtga tagttgccgc attcaccaga cccggcaatg
    56341 cttccggtaa cagcacttta cggacgatct gcatcggcgt ggcacccatt gcacgggaag
    56401 cttcaattaa cccggttggg atctccagca gagcgttctc gaccatacgg gcaataaacg
    56461 gtgctgcacc aacggttaac ggaacaatcg ctgcctgcaa accaatcgat gtaccaacaa
    56521 taacgcgggt aaacggaatc atccatacca gcaagataat gaacgggata gaacggaaaa
    56581 tgttcacaat cgcagaaacg gtacgataca gcttcgcgtt agcaataatt tgccccggac
    56641 gcgtgacata aagcagaacg ccaaccggca gaccaatcac aaagccaaaa aaaccggata
    56701 cgaaggtcat tgccagcgtt tcccatacgc cacgaaccag cagccacatc atcggctcag
    56761 acataaccca gtacctctac ttttacatgg tgttcctgca gccaggcaat ggcggcttgc
    56821 gtatcttgtt gtgtgccgtg catttcagtc agcatgatgc cgaacttaac gccaccggcg
    56881 tagtccatct gcgcgctaat aatgttgttg ttgacgttga aacgacgcgc ggtttcagaa
    56941 agcagtgggg catcgaccga ttgaccggta aactccagac gcagcatcgg cacgcagtca
    57001 gtaaatggct ccgcttgcag acgttcctgg taatcttccg ggatatccag atgcagggtc
    57061 gactgaataa acttctgcgc cagcggcgtt ttcggatgcg agaacacttc acttaccgtg
    57121 tcctgctcga tcagttcgcc attgctgatg accgccacgc aatcacaaat acgcttcaca
    57181 acgtccattt cgtgggtgat caacagaatc gtcaaaccca gacggcggtt aatgtctttc
    57241 agcagttcga gaatagaacg tgtcgttgcc gggtccagcg cgctggtggc ttcatcacac
    57301 agcaatactt tgggattgct ggctaatgca cgggcaatcg ccacacgttg tttctgccca
    57361 ccggaaagat tcgacgggta gctatcatgc ttatcgccaa gaccaaccaa tgacagcaat
    57421 tccgtcacgc gacgtttgat ctcgtctttc ggtgtgttgt ccagctccag cggcagagcc
    57481 acgttgccaa aaacagtacg cgaagagagc aggttaaaat gctggaaaat cataccaatc
    57541 tggcggcgag ctttggtcaa ctcggattct gacagcgtgg tcagttcctg gccatcgacc
    57601 agcacgctac cctcggttgg gcgctccagc aggtttacac aacgtataag cgtactctta
    57661 cccgcgcctg aggcaccgat aacgccataa atttgtccag ctggcacatg caggctgacg
    57721 ttgttcaacg cctggatgat gcgggtgccc tggtggaaca ctttggtgat attcgaaagt
    57781 tttatcattg attatttatt atcgtcatta agttagtcgt ggcatctcga atgcctgaaa
    57841 cgggcaacgc cgtcaatgaa atggatgtta aggcatccag acgtctaaat caatcaggtt
    57901 tatgcgaaga gcactttctt gcaggtcgaa acatgcgata ctagcgtcac atgccttatt
    57961 aaggagctat aaaaggtggc aaagagcgta cccgcaattt ttcttgaccg tgatggcacc
    58021 attaatgtcg atcacggcta tgtccatgag atcgacaact ttgaatttat cgacggtgtt
    58081 attgacgcca tgcgcgagct aaaaaaaatg ggctttgtgc tggtggtagt aaccaaccag
    58141 tctggcattg ctcgcggtaa atttaccgaa gcacagtttg aaacgctgac cgagtggatg
    58201 gactggtcgc tggcggaccg agatgtcgat ctggatggta tctattattg cccgcatcat
    58261 ccgcagggta gtgttgaaga gtttcgccag gtctgcgact gccgtaaacc acatccgggg
    58321 atgcttttgt cagcacgcga ttatttgcat attgatatgg ccgcttctta tatggtgggc
    58381 gataaattag aagatatgca ggcagcggct gcggcgaacg tgggaacaaa agtgctggtg
    58441 cgtacgggta aacctattac gcctgaagca gaaaacgcgg cggattgggt gttaaatagc
    58501 ctggcagacc tgccgcaagc gataaaaaag cagcaaaaac cggcataatg attaaaagat
    58561 gagcgattga aataaaaatg catttttcca cttgtcttcc tgagacgact ccctataatg
    58621 cgcctccatc gacacggcgg atgtgaatca cttcacacaa acagccggtt cggttgaaga
    58681 gaaaaatcct gaaattcagg gttgactctg aaagaggaaa gcgtaatata cgccacctcg
    58741 cgacagtgcg ctaaagtgcg tcgcaactgc tctttaacaa tttatcaggc aatctgtgtg
    58801 ggcactcgaa gatacggatt cttaacgtcg caaggcgaaa aatg
//
LOCUS       DAZPEB010000006        47217 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZPEB010000006 DAZPEB010000000
VERSION     DAZPEB010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47217
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.006"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            27..764
                     /locus_tag="ACHURJ_000357"
     CDS             27..764
                     /locus_tag="ACHURJ_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFR1279442.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLANTSQESWLINSKINRPTRWAGGKASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYDGLSAKKEQNLP"
     gene            875..1885
                     /gene="pyrD"
                     /locus_tag="ACHURJ_000358"
     CDS             875..1885
                     /gene="pyrD"
                     /locus_tag="ACHURJ_000358"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFR1279443.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            2059..2601
                     /gene="zapC"
                     /locus_tag="ACHURJ_000359"
     CDS             2059..2601
                     /gene="zapC"
                     /locus_tag="ACHURJ_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFR1279444.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNVLVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(2598..3707)
                     /gene="ycbX"
                     /locus_tag="ACHURJ_000360"
     CDS             complement(2598..3707)
                     /gene="ycbX"
                     /locus_tag="ACHURJ_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFR1279445.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     ILEGEVSPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            3951..6059
                     /gene="rlmKL"
                     /locus_tag="ACHURJ_000361"
     CDS             3951..6059
                     /gene="rlmKL"
                     /locus_tag="ACHURJ_000361"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFR1279446.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPLKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYSDWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGALSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            6071..7978
                     /locus_tag="ACHURJ_000362"
     CDS             6071..7978
                     /locus_tag="ACHURJ_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1279447.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIVLIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNIAEGKQEVMVNGKPRHVLSYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            8108..9361
                     /gene="pqiA"
                     /locus_tag="ACHURJ_000363"
     CDS             8108..9361
                     /gene="pqiA"
                     /locus_tag="ACHURJ_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFR1279448.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            9366..11006
                     /gene="pqiB"
                     /locus_tag="ACHURJ_000364"
     CDS             9366..11006
                     /gene="pqiB"
                     /locus_tag="ACHURJ_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFR1279449.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENTDVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            11003..11566
                     /gene="pqiC"
                     /locus_tag="ACHURJ_000365"
     CDS             11003..11566
                     /gene="pqiC"
                     /locus_tag="ACHURJ_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFR1279450.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSVQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            11822..11989
                     /gene="rmf"
                     /locus_tag="ACHURJ_000366"
     CDS             11822..11989
                     /gene="rmf"
                     /locus_tag="ACHURJ_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFR1279451.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(12059..12577)
                     /gene="fabA"
                     /locus_tag="ACHURJ_000367"
     CDS             complement(12059..12577)
                     /gene="fabA"
                     /locus_tag="ACHURJ_000367"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFR1279452.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(12646..14406)
                     /locus_tag="ACHURJ_000368"
     CDS             complement(12646..14406)
                     /locus_tag="ACHURJ_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR1279453.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            14592..15044
                     /gene="matP"
                     /locus_tag="ACHURJ_000369"
     CDS             14592..15044
                     /gene="matP"
                     /locus_tag="ACHURJ_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFR1279454.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKLDLQALLGKE"
     gene            complement(15120..16160)
                     /gene="ompA"
                     /locus_tag="ACHURJ_000370"
     CDS             complement(15120..16160)
                     /gene="ompA"
                     /locus_tag="ACHURJ_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFR1279455.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(16517..17026)
                     /gene="sulA"
                     /locus_tag="ACHURJ_000371"
     CDS             complement(16517..17026)
                     /gene="sulA"
                     /locus_tag="ACHURJ_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFR1279456.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSSWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            17245..17874
                     /gene="sxy"
                     /locus_tag="ACHURJ_000372"
     CDS             17245..17874
                     /gene="sxy"
                     /locus_tag="ACHURJ_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFR1279457.1"
                     /translation="MKSLSYKRIYKSQEYLAMLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(17837..19999)
                     /gene="yccS"
                     /locus_tag="ACHURJ_000373"
     CDS             complement(17837..19999)
                     /gene="yccS"
                     /locus_tag="ACHURJ_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFR1279458.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGFWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(20009..20455)
                     /locus_tag="ACHURJ_000374"
     CDS             complement(20009..20455)
                     /locus_tag="ACHURJ_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFR1279459.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            20578..22632
                     /gene="helD"
                     /locus_tag="ACHURJ_000375"
     CDS             20578..22632
                     /gene="helD"
                     /locus_tag="ACHURJ_000375"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFR1279460.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GKVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(22664..23122)
                     /gene="mgsA"
                     /locus_tag="ACHURJ_000376"
     CDS             complement(22664..23122)
                     /gene="mgsA"
                     /locus_tag="ACHURJ_000376"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFR1279461.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(23218..23880)
                     /locus_tag="ACHURJ_000377"
     CDS             complement(23218..23880)
                     /locus_tag="ACHURJ_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFR1279462.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            24053..24466
                     /locus_tag="ACHURJ_000378"
     CDS             24053..24466
                     /locus_tag="ACHURJ_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFR1279463.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(24511..24828)
                     /gene="hspQ"
                     /locus_tag="ACHURJ_000379"
     CDS             complement(24511..24828)
                     /gene="hspQ"
                     /locus_tag="ACHURJ_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFR1279464.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(24886..26076)
                     /gene="rlmI"
                     /locus_tag="ACHURJ_000380"
     CDS             complement(24886..26076)
                     /gene="rlmI"
                     /locus_tag="ACHURJ_000380"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFR1279465.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALVSALQTLYPECAIYDRSDVA
                     VRKKEGMELTLGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            26171..26449
                     /gene="yccX"
                     /locus_tag="ACHURJ_000381"
     CDS             26171..26449
                     /gene="yccX"
                     /locus_tag="ACHURJ_000381"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFR1279466.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(26446..26775)
                     /gene="tusE"
                     /locus_tag="ACHURJ_000382"
     CDS             complement(26446..26775)
                     /gene="tusE"
                     /locus_tag="ACHURJ_000382"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFR1279467.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(26866..27525)
                     /gene="yccA"
                     /locus_tag="ACHURJ_000383"
     CDS             complement(26866..27525)
                     /gene="yccA"
                     /locus_tag="ACHURJ_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFR1279468.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIVHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(27732..27819)
                     /locus_tag="ACHURJ_000384"
     tRNA            complement(27732..27819)
                     /locus_tag="ACHURJ_000384"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27783..27785),aa:Ser,seq:tga)
     gene            28246..29364
                     /gene="hyaA"
                     /locus_tag="ACHURJ_000385"
     CDS             28246..29364
                     /gene="hyaA"
                     /locus_tag="ACHURJ_000385"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFR1279469.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            29361..31154
                     /gene="hyaB"
                     /locus_tag="ACHURJ_000386"
                     /pseudo
     CDS             29361..31154
                     /gene="hyaB"
                     /locus_tag="ACHURJ_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
     gene            31173..31880
                     /gene="hyaC"
                     /locus_tag="ACHURJ_000387"
     CDS             31173..31880
                     /gene="hyaC"
                     /locus_tag="ACHURJ_000387"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFR1279470.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDGTVISTMV
                     NGYRSHKFGKISNKECS"
     gene            31877..32464
                     /gene="hyaD"
                     /locus_tag="ACHURJ_000388"
     CDS             31877..32464
                     /gene="hyaD"
                     /locus_tag="ACHURJ_000388"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFR1279471.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLLAAEQSALAQLAAWGSVP
                     QLANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            32461..32859
                     /gene="hyaE"
                     /locus_tag="ACHURJ_000389"
     CDS             32461..32859
                     /gene="hyaE"
                     /locus_tag="ACHURJ_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFR1279472.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGVFRFPTTLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            32856..33713
                     /locus_tag="ACHURJ_000390"
     CDS             32856..33713
                     /locus_tag="ACHURJ_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFR1279473.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            33847..35391
                     /gene="appC"
                     /locus_tag="ACHURJ_000391"
     CDS             33847..35391
                     /gene="appC"
                     /locus_tag="ACHURJ_000391"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFR1279474.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWAFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            35403..36539
                     /gene="appB"
                     /locus_tag="ACHURJ_000392"
     CDS             35403..36539
                     /gene="appB"
                     /locus_tag="ACHURJ_000392"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFR1279475.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLSSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            36552..36644
                     /gene="cbdX"
                     /locus_tag="ACHURJ_000393"
     CDS             36552..36644
                     /gene="cbdX"
                     /locus_tag="ACHURJ_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFR1279476.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            36724..38022
                     /gene="appA"
                     /locus_tag="ACHURJ_000394"
     CDS             36724..38022
                     /gene="appA"
                     /locus_tag="ACHURJ_000394"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFR1279477.1"
                     /translation="MKAILIPFLSLLIPLTSQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYFGHYQRQRLVADGLLAKKGCPQP
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(38137..40317)
                     /gene="etk"
                     /locus_tag="ACHURJ_000395"
     CDS             complement(38137..40317)
                     /gene="etk"
                     /locus_tag="ACHURJ_000395"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFR1279478.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(40337..40783)
                     /gene="etp"
                     /locus_tag="ACHURJ_000396"
                     /gene_synonym="yccY"
     CDS             complement(40337..40783)
                     /gene="etp"
                     /locus_tag="ACHURJ_000396"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFR1279479.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(40771..41910)
                     /locus_tag="ACHURJ_000397"
     CDS             complement(40771..41910)
                     /locus_tag="ACHURJ_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFR1279480.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTRNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(41956..44052)
                     /locus_tag="ACHURJ_000398"
     CDS             complement(41956..44052)
                     /locus_tag="ACHURJ_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFR1279481.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVNDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(44052..44798)
                     /locus_tag="ACHURJ_000399"
     CDS             complement(44052..44798)
                     /locus_tag="ACHURJ_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFR1279482.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVDPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(44795..45439)
                     /gene="gfcB"
                     /locus_tag="ACHURJ_000400"
     CDS             complement(44795..45439)
                     /gene="gfcB"
                     /locus_tag="ACHURJ_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFR1279483.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(45546..45851)
                     /locus_tag="ACHURJ_000401"
     CDS             complement(45546..45851)
                     /locus_tag="ACHURJ_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-rich inner membrane protein GfcA"
                     /protein_id="HFR1279484.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(46293..46505)
                     /gene="cspH"
                     /locus_tag="ACHURJ_000402"
     CDS             complement(46293..46505)
                     /gene="cspH"
                     /locus_tag="ACHURJ_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFR1279485.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            46791..47003
                     /gene="cspG"
                     /locus_tag="ACHURJ_000403"
     CDS             46791..47003
                     /gene="cspG"
                     /locus_tag="ACHURJ_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFR1279486.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            47014..47202
                     /locus_tag="ACHURJ_000404"
     CDS             47014..47202
                     /locus_tag="ACHURJ_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFR1279487.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVTLQIRTDHAPLDFTK"
BASE COUNT        11625 a        11731 c        12412 g        11449 t
ORIGIN      
        1 tagcgatgtg cagtcgggta aagtgaatgc cagcgcgaca tttgtacttc attatgacta
       61 atacgtggaa cagattggcg ctcttgattt tcgccgtttt atcgctgctg gtggcgggtg
      121 aattgcgggc aggagtggtg gtcggcggaa cgcgatttat ctttccggca gacagagaat
      181 cgatatctat tttactggct aataccagtc aggaatcctg gctgattaac agtaaaatca
      241 accgcccaac gcgttgggcc ggggggaaag cgtcgacagt gccagcacca ttactggccg
      301 ctccgccact tattctcctg aagcccggta ctacaggcac gttgcgcttg ctgagaacgg
      361 aaagcgacat cttgcctgtg gatcgcgaaa cgctatttga gttaagcatt gccagcgtgc
      421 catccggcaa agttgaaaat cagagcgtaa aagtggcgat gcgctcggta tttaaactgt
      481 tctggcgacc cgaaggtttg ccgggcgacc cgctggaagc ttatcaacaa ttacgctgga
      541 cacggaattc gcagggtgta caactcacta acccaacgcc ttactacatt aacctgattc
      601 aggtgagcgt aaatggtaaa gcgttaagta atgcgggagt tgtgccgcct aaaagccagc
      661 gtcagacaag ctggtgtcag gcaatcgcac cgtgtcatgt cgcctggcgg gcgataaatg
      721 attacgacgg gttgagtgca aagaaggagc aaaatctgcc ctgaaacagg ttcggaaaac
      781 gtttgcgttt tttttgccgc aggtcaattc ccttttggtc cgaactcgca cataatacgt
      841 ccccggtttg cacaccggga atccaggaga gttcatgtac taccccttcg ttcgtaaagc
      901 ccttttccag ctcgatccag agcgcgctca tgagtttact tttcagcaat tacgccgtat
      961 tacaggaacg ccgtttgaag cactggtgcg gcagaaagtg cctgcgaaac ctgttaactg
     1021 catgggcctg acgtttaaaa atccgcttgg tctggcagcc ggtcttgata aagacgggga
     1081 gtgcattgac gcgttaggcg cgatgggatt tggatcgatc gagatcggta ccgtcacgcc
     1141 acgtccacag ccaggtaatg acaagccgcg tctctttcgt ctggtagatg ccgaaggttt
     1201 gatcaaccgt atgggcttta ataatcttgg cgttgataac ctcgtagaga acgtaaaaaa
     1261 ggcccattat gacggcgtcc tgggtattaa catcggcaaa aataaagata cgccagtgga
     1321 gcagggcaaa gatgactatc tgatttgtat ggaaaaaatc tatgcctatg cgggatatat
     1381 cgccatcaat atttcatcgc cgaatacccc aggattacgc acgctgcaat atggtgaagc
     1441 gctggatgat ctcttaaccg cgattaaaaa taagcaaaat gatttgcaag cgatgcacca
     1501 taaatatgtg ccgatcgcag tgaagatcgc gccggatctt tctgaagaag aattgatcca
     1561 ggttgccgat agtttagttc gccataatat tgatggcgtt attgcaacca ataccacact
     1621 cgatcgttct cttgttcagg gaatgaaaaa ttgcgatcaa accggtggct taagtggtcg
     1681 tccgcttcag ttaaaaagca ccgaaattat tcgccgcttg tcactggaat taaacggtcg
     1741 cttaccgatc atcggtgttg gcggcatcga ctcggttatc gctgcgcgtg aaaagattgc
     1801 tgcgggtgcc tcactggtgc aaatttattc tggttttatt tttaaaggtc cgccgctgat
     1861 taaagaaatc gttacccata tctaattatt tcttcgaatt cctctttata agataatcag
     1921 ggctttattt tcagccctgg ttgttttata ttcatcgctg ttgcttattt agacattttg
     1981 tacttttatt gttgaggtta ttaagcgaag cgacaatgga ttgtgttgct gcggttttat
     2041 agggtagggg agaggcagat gcgaattaaa ccagacgata actggcgttg gtattacgat
     2101 gaagagcacg atcgaatgat gctcgattta gccaatggta tgctatttcg ctcacgtttt
     2161 gcgcgcaaga tgttgactcc agatgccttt tctcccgcag gcttttgcgt tgacgacgcc
     2221 gcgctctatt tctcttttga agaaaagtgc cgcgatttta atttatctaa agaacaaaaa
     2281 gccgaactgg tgctgaatgt actggtagca attcgctatc tcaaaccgca aatgccgaaa
     2341 agctggcatt ttgtttccca tggtgaaatg tgggtgccca tgccgggcga tgcggcctgc
     2401 gtttggttga gcgatactca cgagcaggtc aatttgctgg tcgttgaatc tggcgaaaat
     2461 gccgcactat gcctgctggc acaaccctgc gttgttattg cgggtcgcgc gatgcagttg
     2521 ggcgacgcca ttaaaatcat gaacgacagg ctgaaaccgc aggttaatgt tgacagcttc
     2581 agcctcgaac aggcagtcta acgcgccaac ttaagtgcag tcttcggtac acagctacag
     2641 caaagaatgg tgccatcatc gcccattgct gattttttca gcggcgagac ttcgccttct
     2701 aaaatctgaa cacggcaact tccacaaatg cccgcgcggc aagaataagg gatacgaatt
     2761 ccctgatttt ctaattgctc cagcaacacc tgttggttat ttccacgaaa tgcctgtccc
     2821 tgccagtcaa tatctacatt tgcgtccggt tgttgcgtga tgttggcggt atcatcagcg
     2881 gcagctgcgc cgtaaatttt tgccggagcc gttgccagaa tttccacctc atcgccaacg
     2941 cgaatcacgc cgctattacg ggcaattaaa ttctgaccaa aatcgacatc gccgttatcc
     3001 tgggcagtgc ggaaagattg caatgttttt aatggttcgc ctgccggatg tttttgccct
     3061 ttttctgggc tgacggtggt gaaaatacag cggctacaag gtttaaccac atcaaacacc
     3121 acatcaccaa tgcgaatcac tttccagcga tcttcttccc aggctgacgc gccggaaacc
     3181 accagattgg ggcggaattg ctccattttt acactggccg gacaacgttg ttggagatca
     3241 cgtaacgagg cttcgttagc aagaaggtaa ggatagccat cagcaaatga cagaggtaca
     3301 gtgttgtggc gtttcacgcg ccgggtcatt tgtggcccca cccagcgtaa ttgcacttcg
     3361 cgggagaaaa atccacttag ccatttgttg atcgcgtctg gcgcaattcg cgcggtaaaa
     3421 tgtgtgcccc aaacttcggt tggtgcgtct tgtgtggcga aatcagcaaa acgaacatat
     3481 gcgctactgc catctggtgc ggtgagatgc aagccatcat gcacaggtga aggagtaaac
     3541 cgtaccatct ggggaaactg gcgagcggta ataaacgtac cgtcaggttc cgtgatcata
     3601 aagatgcgat cgaaggccag accactgaca tcagccagag catgtgtaag accaatgccg
     3661 cgcatcgatt taacaggatg aataaaaagc cgggttaatg tcgccacggt gcggtcctca
     3721 aatgaaaata agccctcaac tttatgacat gacgcgctta ttagctataa tgcgcaacaa
     3781 ttttcttagc gcctgtccca ttaggctatt ttatttgtca gtttggccct gggttgtgct
     3841 cgaaatcctc acgtacttgt gtacgctccg gtttctccgc gctgtccatg tccagactga
     3901 ctgcaacaat tacgcctact gcggtaggct cttagagtaa aagtgacgat atgaattctc
     3961 tgtttgccag tacggcccgt gggctggaag agctgttaaa aactgaactg gaaaacctgg
     4021 gggccgttga atgccaggtg gttcagggtg gggtccattt caagggcgac acacggcttg
     4081 tttaccagag cctgatgtgg agccgcctgg cctcgcgtat tatgttgccg ctgggcgagt
     4141 gtaaggttta cagcgattta gacctctatc tcggtgttca ggcgatcaac tggacagaga
     4201 tgtttaatcc tggcgcgacc ttcgctgtcc acttcagtgg tttgaatgac accatccgca
     4261 acagtcagta cggcgcgatg aaagtgaaag acgcgatcgt cgatgctttc acgcggaaaa
     4321 atctgccgcg tccaaatgtt gatcgcgatg cgccggatat ccgcgttaac gtctggctgc
     4381 ataaagaaac cgccagtatc gctcttgatc tgagtggtga tggtttacat ctgcgtggct
     4441 atcgcgatcg tgctggtatt gcgccgctca aagaaaccct ggcagccgct attgtgatgc
     4501 gatccggctg gcagcctgga acaccgctgc tcgatccgat gtgtggttcc ggtacgttgc
     4561 tgattgaagc ggcgatgctg gcgaccgatc gcgcacccgg cttgcaccgt gggcgttggg
     4621 gctttagcgg ctgggcgcag catgatgaag ctatctggca ggaagtgaaa gcggaagcgc
     4681 aaactcgcgc ccgtaaaggc ctggctgagt atagctctca tttttacggt tcggacagcg
     4741 acgcacgggt gattcaacgt gcacgcacta acgcccgtct tgcggggatt ggtgaactga
     4801 tcacctttga ggtgaaagat gtcgcgcaac tgaccaatcc gctgccgaaa gggccgtacg
     4861 gcacagtgtt gagcaacccg ccatacgggg aacgtctgga cagcgaaccg gcgctgattg
     4921 cgctgcatag cctgctgggt cggatcatga aaaaccagtt tggtggctgg aatctctctt
     4981 tgtttagtgc ctcgccggat ctgctaagct gcttgcagct gcgtgcagac aaacagtaca
     5041 aggcgaaaaa cggcccgctg gactgcgtac agaaaaatta ccacgttgcc gaaagcactc
     5101 cagacagcaa accggcgatg gtagcggaag actacgccaa ccgcctgcgt aagaacctca
     5161 aaaaattcga gaagtgggct cgccaggaag gaattgaatg ttaccgcctg tatgacgccg
     5221 atctgccgga atataacgtt gccgttgacc gttattccga ctgggtggtg gtgcaggagt
     5281 atgcaccgcc aaaaactatt gatgctcaca aagcgcgtca gcgtctgttc gatattatcg
     5341 ctgcaaccat ttcggtactg gggattgcgc caaacaaact ggtgctgaaa acccgtgaac
     5401 gccagaaggg caaaaaccag taccagaaac tgggcgagaa gggcgagttt cttgaagtta
     5461 ccgaatataa cgctcacttg tgggtgaacc tgacggatta tctcgatact ggtctgttcc
     5521 tcgatcaccg tatcgcccgc cgtatgctcg gtcagatgag caaaggcaaa gatttcctca
     5581 acctgttctc ttataccggt agcgccaccg tgcatgcggg attaggcggc gcgctcagca
     5641 ccaccaccgt ggatatgtcg cgtacttatc tggagtgggc agaacgcaac ctgcgtctga
     5701 atggcctaac cgggcgtgcg catcgcctga ttcaggccga ttgcctggcg tggctgcgtg
     5761 aggcaaatga acagtttgat ctgatcttta tcgatccgcc aaccttctct aactcaaaac
     5821 gaatggaaga tgcgtttgat gttcagcgcg atcatctggc gctgatgaaa gatttgaaac
     5881 gtctgctgcg tgcaggtggg acgatcatgt tctcgaacaa caaacgtggc ttccgtatgg
     5941 atctcgacgg cctggcaaaa ctgggactga aagcacaaga aattacgcaa aaaacgctct
     6001 cccaggattt cgcccgtaac cgccagatcc acaactgctg gctgattacc gcagcctgaa
     6061 aggaatagta atgtcattaa tcagtatgca tggcgcatgg ctgtcgttca gcgacgcgcc
     6121 gcttctcgat aacgcagaac tgcatatcga agataacgaa cgtgtttgtc tggtgggccg
     6181 caacggcgcg ggcaaatcga cgttaatgaa aatcctcaac cgtgaacaag ggctggatga
     6241 cggtcgcatt atttacgagc aagatttgat tgtagctcgt ctgcaacagg atccgccgcg
     6301 taacgttgag ggtagcgttt atgatttcgt tgccgaaggc attgaagaac aagcggaata
     6361 tctgaaacgc tatcacgata tttcgcgcct ggtgatgaac gacccgagcg agaaaaatct
     6421 caacgaactg gcgaaggttc aggaacagtt ggatcaccac aacctgtggc agctggaaaa
     6481 ccgcatcaac gaagtgctgg cgcaactggg gttagatcct aatgttgcgc tgtcgtcgct
     6541 ttccggcggc tggttgcgta aagcggcatt aggacgcgcg ctggtgagta atccgcgcgt
     6601 gctgttgctt gatgaaccga caaaccacct ggatattgaa accatcgact ggctggaagg
     6661 gttccttaaa accttcaatg ggacgattat tttcatctcc cacgaccgtt cgtttatccg
     6721 caatatggcg acgcgcattg ttgatctcga tcgcggcaag ctggtgacct atccagggaa
     6781 ttacgaccag tacctgcttg aaaaagaaga agccctgcgc gtggaagaac tacaaaatgc
     6841 cgagttcgat cgcaaactgg cgcaggaaga ggtgtggatc cgccagggga tcaaagcacg
     6901 ccgtacccgt aatgaaggcc gcgtacgcgc cctgaaagca atgcgtcgcg agcgtggtga
     6961 acgtcgcgaa gtgatgggta ccgcaaagat gcaggtggaa gaggccagcc gctccggtaa
     7021 gatcgttttc gaaatggaag acgtttgcta ccaggttgac ggtaagcaac tggtgaaaga
     7081 tttttcggcc caggttctac gtggcgacaa aattgtcctg attggtccga atgggtgcgg
     7141 caaaaccacg ctgctgaaac tgatgctcgg tcagcttcaa gcggacagcg ggcgtattca
     7201 cgttggcacc aaactggaag tggcttattt cgatcagcac cgcgcggaac tggatcccga
     7261 taaaacggtg atggataaca ttgccgaagg taagcaagag gtgatggtta acggcaagcc
     7321 acgccacgta ttgagctatt tgcaggactt tctgttccat ccgaaacggg cgatgacgcc
     7381 ggtacgtgcg ctttctggcg gtgagcggaa ccgcttgctg ctggcgcgtt tgttcctcaa
     7441 accaagcaac ttattgattc ttgacgaacc gaccaacgat cttgatgtcg aaacgctgga
     7501 actgctggaa gaactgatcg acagctatca gggcacggta ttgctggtta gccacgatcg
     7561 tcagtttgtc gataacaccg ttacagaatg ttggatcttc gaaggcggcg gtaaaattgg
     7621 tcgttatgtc ggcggttatc atgatgcccg tggtcagcaa gagcagtatg tggcgctcaa
     7681 acagcctgcg gtgaaaaaaa ccgaagaagc cgccgcggca aaagcagaaa ctgtaaaacg
     7741 cagcagtagc aaactaagct ataaattgca gcgcgaactg gagcagctac cgcaattgct
     7801 cgaagatctg gaggcgaagc tggaagccct acagacgcag gtggcggatg cttccttctt
     7861 cagtcagcca catgagcaga cacaaaaagt gctcgccgat atggctgctg cagagcagga
     7921 gctggagcaa gcctttgaac gctgggagta tcttgaagcg ttaaaaaatg gtggctgatc
     7981 gcaaaatagt cgatttaaac agagacagct gacaacggta aaattgcctg atgaactggg
     8041 tttatcaggc atgaaaacca acactttgtt agtcatctgt aaaatagcgc atcattaagg
     8101 agtaccaatg tgcgaacatc atcatgccgc gaagcacatc ctgtgctcgc agtgtgacat
     8161 gctggtggcg ttaccgcgcc ttgagcatgg tcagaaagcg gcatgtcccc ggtgtggcac
     8221 aacgttaacc gtggcgtggg atgcccctcg gcagcgtccg accgcctatg cgttggctgc
     8281 actgttcatg ctgttgctgt ccaacttgtt tccttttgtg aatatgaacg ttgcaggagt
     8341 taccagtgaa attacattac tggaaattcc cggcgtgctt ttttctgagg actacgccag
     8401 cctcggcacc tttttcctgt tgtttgtgca actggttccc gcgttttgtc tgataaccat
     8461 tctgttactg gtgaatcgcg cggaattacc ggtccgttta aaagagcaac tggcacgggt
     8521 gctttttcaa ctcaaaacct ggggaatggc ggagattttc ctcgcgggtg tgctggtcag
     8581 tttcgttaaa ctgatggctt acggcagcat tggggtaggc agcagctttc tcccctggtg
     8641 tttattttgt gtcctgcaac tgcgcgcttt tcagtgcgtt gatcgtcgct ggttatggga
     8701 cgacatcgcc ccgatgccag aactgcgcca gccgctaaaa ccaggcgtca cggggatacg
     8761 tcaggggctg cgttcgtgct cctgttgtac ggcaatcctt cctgctgatg aacccgtgtg
     8821 cccgcgttgt agtaccaaag ggtacgttcg gcgtagaaac agcctgcagt ggacactcgc
     8881 gctgcttgta acgtccatca tgctgtatct tccggctaat attttgccca tcatggtgac
     8941 ggatttatta ggctcgaaga tgccgtcgac gattctcgct ggggtcattc tgttatggag
     9001 cgaaggctct tatcccgtcg ctgcggtgat ctttctggcc agtattatgg tgccaacgtt
     9061 aaagatgatc gccatcgcgt ggctgtgttg ggatgccaaa gggcatggca agcgcgacag
     9121 tgaaagaatg catttgattt atgaagttgt tgagtttgta ggccgctggt cgatgattga
     9181 cgttttcgtt atcgcggtgc tctcggcgct ggtgcgtatg ggaggtttaa tgagtattta
     9241 tccggcaatg ggtgcattaa tgtttgcttt agtcgtcata atgacaatgt tttctgctat
     9301 gacgtttgac ccgcgtttgt cgtgggatcg tcaacctgaa tcagagcatg aggagtcctg
     9361 acagtatgga atctaataat ggggaagcca aaatccagaa agtgaagaac tggtctcccg
     9421 tgtggatatt tcctatcgtc acggcgctca ttggggcctg ggttctattt tatcattaca
     9481 gccatcaggg accggaagtg accctgatca ccgcgaatgc ggaaggaatt gaaggtggca
     9541 aaaccaccat taaaagccgt agcgttgacg tcggcgtggt tgaaagcgcc acactggctg
     9601 atgatttgac gcacgttgaa atcaaagcgc ggctgaattc cggtatggaa aaattgctgc
     9661 ataaagacac cgtcttttgg gtggtgaaac cgcagattgg tcgcgaaggg attagcggcc
     9721 tgggaacgct gctgtctgga gtttatatcg aactgcagcc aggcgcgaaa ggcagcaaaa
     9781 tggataaata cgatttgctg gactcgccac cgttggcccc gcctgatgcg aaaggtatcc
     9841 gtgtgattct cgatagcaaa aaagccgggc agctctcgcc aggagatccg gtgctgttcc
     9901 gtggctatcg ggtaggttcg gttgaaacca gcaccttcga tacgcaaaaa cgcaatatca
     9961 gttatcaact gttcatcaat gcaccttatg accgactggt gaccagcaat gttcgcttct
    10021 ggaaagatag tggcattgcg gttgatctga cgtcagcggg gatgcgtgtg gagatgggct
    10081 cattgacaac gctgcttagt ggcggtgtta gctttgatgt gccggaaggt ctggatttag
    10141 ggcagccagt ggcaccgaaa acagctttcg ttttgtatga tgatcagaag agcattcagg
    10201 attcgttgta caccgatcac attgattatc tgatgttctt taaagattcg gtacgcggtc
    10261 tgcaaccggg agctccggta gaattccggg gtattcgcct gggtaccgta agcaaagtgc
    10321 cattctttgc gccgaatatg cgtcagacat ttaacgatga ttaccgtatt ccggtactga
    10381 ttcgtatcga gccagagcgg ctgaaaatgc agcttggcga aaatacggat gttgttgagc
    10441 accttggcga attgttgaaa cgtggtttac gcggatcgct gaaaaccgga aacctggtca
    10501 ctggtgcact gtatgttgat ctcgatttct atccaaatac gcctgcaata accggtattc
    10561 gtgaatttaa tggttatcag attatcccga ctgttagcgg cggcctggcg caaatccagc
    10621 aacgactgat ggaagcgttg gataagatca acaaactgcc attgaatccg atgattgaac
    10681 aggcaaccag tacgctttct gaaagtcagc gcacaatgaa aaacctgcaa acgacgctgg
    10741 atagcatgaa caagatcctc gctagccagt cgatgcagca gttgccgacg gatatgcagt
    10801 caacgttgcg tgaattgaat cgcagcatgc agggcttcca gcctggctcc gcagcctaca
    10861 acaagatggt ggcggatatg cagcgccttg atcaggtgtt gcgagaactg caaccggtgc
    10921 tgaaaacgct caatgagaag agtaacgcgc tggtatttga agcgaaggac aaaaaagatc
    10981 cagagccgaa gagggcgaaa caatgaaaaa gtggctagtg acgattgcag cactgtggct
    11041 ggccggatgc agctccggcg aaattaataa aaactattac cagttacctg tggtgcagag
    11101 cggtacacaa agtaccgcca gccagggcaa tcgtctgtta tgggtagagc aggtcactgt
    11161 tcctgactat ctggcgggga atggtgtggt ttatcaaacc agtgatgtga agtatgtgat
    11221 tgccaacaac aacttgtggg ccagcccgtt ggatcaacag ttgcgcaaca ccctggttgc
    11281 caacctgagt acgcaactgc ccggctgggt ggttgcctcc cagcctctgg gaagcgtcca
    11341 ggacacgctc aatgttaccg taacggagtt taacggtcgc tatgatggca aggtcattgt
    11401 cagtggtgag tggctgttga accaccaggg acaactgatc aaacgtccgt tccgtctgga
    11461 aggagtgcaa actcaggatg gttacgatga gatggttaaa gtgctggccg gtgtctggag
    11521 tcaggaagcc gcttctattg cacaagagat aaagcgtcta ccttaattat aaagatttgt
    11581 aagtataacc gtctccggta tgttgcctga ggcggttttt ttgtttctaa cgtgcggaaa
    11641 aatttgttcc tcttcacatt ttttgtacaa ccgacatgcc cgtgtagctc acaaatatga
    11701 cagtggcgtg aattttgcgc attgacggca gttatgattc gcggtattgc ttaactgtga
    11761 ttgcacattt agtaatcact gttttctttt ccaccagaaa ccagtatgag ggaaacgagg
    11821 catgaagaga caaaaacgag atcgcctgga acgggcacat caacgtggtt atcaggccgg
    11881 catcgccgga cgctcaaaag aaatgtgtcc ctatcagacg ctgaatcaaa ggtcacaatg
    11941 gctgggaggc tggcgagaag ccatggcgga cagggtagta atggcctgat tctgtctctt
    12001 taaaaagaaa cctccgcatt gcggaggttt cgccttttga tactctgtct gatgataatc
    12061 agaaggcaga cgtatcctgg aacagaccga ctttcaggtc gctggcggta tagatcagac
    12121 gaccatcaac cagcacttcg ccatctgcca gacccataat cagacgacga ttaacaatgc
    12181 gtttaaagtg aatacggtag gtcacttttt tcgctgtcgg cagtacctga ccagtgaatt
    12241 tcacttcgcc aacgcccagc gcgcggcctt taccttcgcc gcccagccag ccgaggtaga
    12301 accctactag ctgccacatt gcgtccaggc ccaggcatcc cggcataacc ggatcgccaa
    12361 tgaagtggca tccgaagaac cacagatccg gattgatatc cagttctgct tcaacatacc
    12421 ctttgtcgaa gttaccaccc gtttcggtca ttttgaccac acggtccatc atcagcatgt
    12481 tcggtgctgg caattgcggg cctttagcgc caaacagttc accgcgacca gaggcaagaa
    12541 ggtcttcttt tgtataggat tcgcgtttat ctaccatgtt ctctgtaagc cttattttat
    12601 tgaagcacgc aggatagcta acacgtgtac gctgaacaag tccgatcagt tcggaataaa
    12661 ccagttcagc caacgtaatg gccatggaaa acggtgacgt ccttcctgtt gcgatgcttg
    12721 cgcgatacgt tcctggatgg tttgcatcag cgtcgtttgg ccttcgccat cccacaccag
    12781 atttaataat aacggcagtg cgtcagtcac atcgtctact gcccagatgg tgaatttgcc
    12841 ttcttctacc gctttcacca gttcactgtg aagacttaaa tggcgaacgt tagctgtggg
    12901 gataatgaca ccttgtttcc cggttaactc acgttgctgg caaatagcaa agaagccttc
    12961 gattttctca tttaaaccac cgaccggctg ggcgcgaccg aactgatcga ctgaacctgt
    13021 gatagcgata ctctgattca ccggcacatc ggcgagggcg cttatcaggg cgcagagttc
    13081 agccatcgag gcactatctc catcaacttc actgtatgac tgctcaaatg tcagcgatgc
    13141 tgagaagggg atctgttgct caagctgtag ttccgacatc aggaacgctt gcatgatcat
    13201 catccctttc gcatggatat tgccgccaag ttccgctttg cgttcgatgt cggtgaattc
    13261 accatcgcca atatgcacaa cgcagctaat gcgagaaggt tcgccaaaag cgcgtggatg
    13321 acccggaaat tcaatgaccg aaagggcgtt aatttgcccg atgcgttcgc cttcggtttc
    13381 aatcaggatt tgctcctgaa ggatctcatc ctgcatacgt tcagcgagga aaccttcgcg
    13441 ccattcacgc tgctgcagca ttaagtttag ctgctcgccg gagaaggtgt cgccatcaca
    13501 cagggaggcg acctctttac actggcggag gatccactgc gggctaagcg gaagtgtttc
    13561 ttgttcaccg gtgtagcgtg ctgcttcgcg gataagtacc ggccaggcat ccgctcccgg
    13621 ggcaggtaag tgattatgtc tggcggtaaa tgtcacccag cgacaccact ggcttactga
    13681 ctccgcatcg acaatctgca gagtatcttc aaattcgcta taaatagcct gctctgaaag
    13741 ctctggctcc atctcctgga aatcagccaa tgattcgcgt tcgcctacca gaatgacttt
    13801 cagcttcaat ggcatcgaag gcacagagac ggggagaggg cgcgactcat caaacgcaac
    13861 ccagtcaaaa cgctcgcggt taacgatatt tttcagccgc atccacagca gaggttgcgc
    13921 cagcagtgta cgcaaagaga tgatgagaat accgccattt gcctgatgca ccagaccagg
    13981 ctgcagggta atgtcgccat taaactggcg caggcagcca aagagttgct ccgcttctac
    14041 ccagtcggca gcgacaactt gcgttaaagt cgcaaaatta tcatctgcac tcactgcgtg
    14101 acgtaagcgg atggagtggc cggaaacctc atagtgatcg cccaccagtt ggcctgcatc
    14161 gctttgtagc gtacgcgcgg catcggcaat aagattcaga tactcagact cttccggggc
    14221 cttagccagc ataaaagagg aggatgctcg cgtatgcagc aactgctcca gcgcaaattg
    14281 cagccgcggt tgagtatcac tgaataaagg atcgttttcg tcaatcaaat gtggctgagc
    14341 aaatatttcc tgatagctat cggtatcagg aaccaggtca cgccatgcaa gtttcgtaat
    14401 ggtcaaagtt gatgtttttt agtctgttgt caaagccgcg attataccgt aaccggcact
    14461 acagcacacg tagaaagcac cgacaatact cctggcatgg gcgttaaagc tcacaggatg
    14521 gagattcttt tcttcactgg cctaaaaagc tgatattctg taaagagtta cacggtaaca
    14581 ttgagatcgc tatgaaatat caacaacttg aaaatcttga aagcggttgg aaatggaagt
    14641 atctggtgaa gaagcatcgc gaaggggagt taattacccg ttacatagaa gccagtgccg
    14701 cccaggaagc cgtcgatgtg ttgctctcgc tggaaaatga acccgtgctg gtaaatggct
    14761 ggattgacaa gcatatgaat ccggaactgg tcaatcgcat gaagcaaacc attcgggcca
    14821 gacggaagcg ccattttaat gcggaacacc agcatacgcg caaaaagtcg atcgatctgg
    14881 aatttattgt ctggcaacgt ctggctggtc ttgcgcagcg tcgcggtaaa acgctttctg
    14941 aaacgattgt tcagctgatt gaagatgcgg aaaacaaaga gaaatacgcg aataaaatgt
    15001 cttctctgaa gctggatctg caggcattgc tgggtaagga ataactgacg aaagtcagtt
    15061 caatttacta aaggcaaaaa aaaccccgca gcagcggggt ttttctacca gacgataact
    15121 taagcctgcg gctgagttac aacgtctttg atacctttaa cttcgatctc tacgcgacga
    15181 tccggagcca ggcagtcgat cagtgcagca cgctgtttca cgttgtcaca ggtgttgcca
    15241 gtaaccgggt tggattcgcc cataccacgt gcggagatct tgtctgccgg gatacctttg
    15301 gagatcaggt aatcaacaac agactgagca cggcgctcgg acagaccctg gttgtaagcg
    15361 tcagaaccga tgcggtcggt gtaacccaga acaactacgg aaccgtcttt cggatccagg
    15421 ttgctcagct ggctgtacag ctgatccaga gcagcctgac cttccggttt cagggttgct
    15481 ttgttgaagt tgaacagaac gtcagacttc agagtgaagt gcttggtctg tacttccggt
    15541 gccggagctg gagccggagc aactactgga gctgcttcgc cctgaccgaa acggtaggaa
    15601 acacccaagc tcagcatgcc gttgtccgga cgagtgccga tggtgtgtgc gtcaccgatg
    15661 ttgttggtcc actggtattc cagacgggta gcgatttcag gagtgatcgc gtactcaaca
    15721 ccgccagcga agaccggaga aacgccggtg tcgtggtttt taccataaac gttggattta
    15781 gtgtctgcac gccataccat accacccaga cgagtgtaga tgtccaggtc gtcagtgatt
    15841 gggtaaccca gtttagcggt cagttgaacg ccctgagctt tgtatgcacc gttttcaacg
    15901 ctgcctttgt acggcatacg acctaaccag tcgtaaccca tttcaaagcc aacatacggg
    15961 ttaacctggt aaccaccaaa agcaccagcg cccagttggt tttcatgggt cgggccattg
    16021 ttgttgatga aaccagtgtc atggtactgg gaccagccca gtttagcacc agtgtaccag
    16081 gtgttatctt tcggagcggc ctgcgctacg gtagcgaaac cagccagtgc cactgcaatc
    16141 gcgatagctg tctttttcat tttttgcgcc tcgttatcat ccaaaatacg ccatgaatat
    16201 ctccaacgag ataacacggt taaatccttc accgggggat ctgctcaata ttaactctac
    16261 cgatatcttc ggcttatgcc gagcacccct ggcgatgtaa agtctacaac gtagttgaaa
    16321 acttacaagt gtgaactccg tcaggcatat gaaaaaaaag tcttgtataa ggtatgttta
    16381 atcttttttg tcagcgacaa tttacagaag aaaatcgcgg aaaccgcttc agacaagcct
    16441 ccgcaaggaa aattagtcac gactgaaagc attggctggg cgacaaaaaa agttccagga
    16501 ttaatcctaa atttacttaa tgatacaaat tagagtgaat ttttagcccg gaaagttgtc
    16561 tcgtggcgtg agaggatgcg cttaccggac gcataataaa ccccatagcg ttaccttcat
    16621 ttgccgcatc aacaagttca gcatgctctt cttcagtcaa atcatctgcc aaccaaccga
    16681 tcaccacact gtaattgccc gtgcgtaaag cgcgaaccat tgactccaca gtgtggcaag
    16741 gggagagctg gctaatctgc attactttcg ttaagggtag cccagatgcc tgaacccatt
    16801 cccgactcag tttttgttgc ggtgttaacc agagttgcca gctcgattgc tgaccgagtt
    16861 gctgtaacaa tggcaacagt agaagttgcg tcatcatggg ctgatcttcg cgatagacaa
    16921 cttcactgat aagcccggct gtagtgtttt ccgtagagac acgcgcaatt ttacttgctg
    16981 cggatgagaa cgacgaagaa cgatgtgcat agcctgaagt gtacataatc aatccagccc
    17041 ctgtgagtta ctgtatgaat gtacagtaca tccagtgaca acaaagatca accctatttt
    17101 cggaaagagc ctcgcaaatt ttttcgttgg tgacgggaaa acataaatta atcttgcccc
    17161 ttaagaataa gttgcctatt ttcgtagtta acggatccgt taatgtgaat cattctttta
    17221 tgttatgatt ttaaaaggaa ttttatgaaa agcctctcct ataagcggat ctataaatca
    17281 caagaatacc tggcaatgtt gggcacaatt gaataccgat cattgtttgg cagttacagc
    17341 ctgaccgttg acgacacggt gtttgcgatg gtttctgatg gtgagttgta tcttcgggct
    17401 tgtgagcaaa gtgcacagta ctgtgtaaaa catccgcctg tctggctgac atataaaaag
    17461 tgtggccgat ccgttaccct taattactat cgggttgatg aaagtctatg gcgaaatcaa
    17521 ctgaagctgg tgcgtctgtc gaaatattct ctcgatgcag cgctgaaaga gaaaagcacg
    17581 cgcaataccc gggaaagact gaaagatttg cccaatatgt cttttcatct ggaagcgatt
    17641 ctcggggagg tggggattaa ggatgtacgg gcgttacgta tacttggggc aaaaatgtgt
    17701 tggttgcgac tgcggcagca aaacagtctg gtgacagaaa agattctgtt tatgcttgaa
    17761 ggtgccatta tcggcattca tgaagctgcg ctcccggtgg cacgccgcca ggagcttgca
    17821 gaatgggctg actcacttac gccgaaacag gagtttcctg cggaacttga gtaatctggc
    17881 gttgcagacg accaatctca ggcagtaatg caatcaataa tccaacttgt tgtacgacca
    17941 gaggttcttt gctgtctgcc cgtggttcaa gttgctgcat ccgctgtttc aggctagcta
    18001 atgcctcatt gacgcgttct tcatcagcag gttgatgatg taacgcgtca tcaacatagc
    18061 aaactgcgtc atcaagaaac gccagaattt caggattagt taactgctcc cggtgagcac
    18121 cgagggctga gatatagctg gtaaacgtat ggttaaggca cagcaaccga aacgcggctt
    18181 cgcgaatttg cggggtaacg ttcggctcgc tggacatatt tgataccacc gacgccagct
    18241 cagcatcacg gttgtgtgca tcgcggcggg caatacgata cgccagacgg ttatcacgcc
    18301 cctgatggta ttgctccagt atggcatcga gataccgaca gttggcctct gtggcgcgtt
    18361 cgagcatgcg cggcaaattg cgaaactgcc agtcaggcca gatgtagctc actgccgccc
    18421 acgcaatggc acaaccaatc agcgtatcga ttacgcgagg taacgctact tcaaaacctt
    18481 cacccagtaa gttaaaacac agtagcacca aaagtgtgat gaacatcgtt gcatgagcgt
    18541 attgcacgtt acggaaggca aaaaagagca cgccggtaat aaccagcagc accagctgcc
    18601 cttccagtga tggcacaaac cacagcacag gaatgccaat ggcgatacct accagcgtac
    18661 caataatcct taacttcagg cggtggcgcg tggcgttata gtttggctgg cagacaaaca
    18721 aacttgtcag caagatccaa aacccgtgat gcattccggt tatttgaatg atggcgtagc
    18781 cgaagcacaa caccagcgac attcttaccg catgacggaa gagggcggat tccggcgtga
    18841 agtgacggct aagacgcagc cagatatcac tcaacccgtg cgggctgtca tcagcgagct
    18901 cattttcgtc attattatgg ggtagtgcct gggcctgttc tgattcaatt gttgccagtt
    18961 gggcatcaat ggcgcgtaaa ttgttcagca aaaatcccag tgttttgagt aaatcggcgg
    19021 gtgcgccgtt atcgcgcatc cgctccagcg cagcatcaat atgcgtaaaa gcgcgctcaa
    19081 aatgcggatc atgttgataa ggctgacgca acaaaataca gcgtgacagt tgctggcacg
    19141 cctggccctg catcgacatc agccgctgaa aacggaacag cacgtcgctg tggcgaaaat
    19201 gttcacgcaa tgtttgatac tgaatatgag aagagctggc acgctcgtga atatcctgtg
    19261 cgacaaagta ataatgcagc gtgcgacgcg ttccccgttg accacgatcg ccacgtaagc
    19321 gggtcagcag cgagagtttc gtctgattca atgtcgccat cagcagaccg ttggcgagag
    19381 ccaaatcgta cagcggtgcc tggctttcat cttcaatatc aggatcaaac atgcgcgact
    19441 tgagctcaag ataacgcgcc agttgttcat agcaacgcgc caggttgtcc tgcagcgggc
    19501 ggaccgggaa cagcagatga ccaataagtg tcaggacgtt gtaccagacg gcaccggcca
    19561 gcagatacat cggctgctga taccagtgct catacagtga tgttcccaac atagtgtaaa
    19621 tggcgatcag caatgcaccg aaggcaattg ttgcatagcg ttgacccaga ccgccgagca
    19681 aaatgaagcc gctggtagag agcgttaagc caatcgcaaa tagccagggc cagggaaaca
    19741 gcaattctac tgaggccgag gcgataaaaa agcagaacag cgtaatgatg aggttacgca
    19801 aacgtcccgc cagtcggtca tcgagatcgg tcagcgccgc tgccaccatc cccagcgtta
    19861 gcggaatcgt cagttttaca tcacccagcc accacggaaa cgctgtggtt ccacaaagcg
    19921 caataaaaat acgcgcgtaa tacagccagg cgctgttcca ggtatagcgt ttgagcaaag
    19981 gacttagcat aaaggccata ttagtggatt attcaaaacg acgacgtgca ttggcttcac
    20041 gcgcagcttg cgctgtttct accgatacca cgcgacgacc aaccggccat agtgcaatag
    20101 cggcaatttt aaagttcgca atgccgacag gaatgccaat gattgaaata cattgtgcga
    20161 tgcccgttgc aatgtgcatc aggcataacc accagccaaa gaaaatcagc cagaaaatat
    20221 tcaataccgt accgccagta ttcagcagca catttttgcc agccgggttc agttcatcga
    20281 catgaatagc ttcattgcca taaggcacca gagacagttt agtgatctcc cagcaggatc
    20341 gtgtcagcgg taaggtaaaa atcagcacaa tactgaccag agtcgccaac agccagccca
    20401 gagtggtggc aaatccgcca agcacaaagt tcagaatgtt caaaacggta cgcataaaac
    20461 ctcgctttac tgtggttttc agtaatgggc ggcaattgta acgttttttt gggctggagc
    20521 acgttttctc tgacggttac actgataaga aataatttcg tgtggatcta cagagtcatg
    20581 gaactgaaag cgacaacgct tggaaaacgt ctggcacagc acccttacga tcgggcggtg
    20641 atcctcaatg ccgggattaa agtctccggc gatcgccacg aataccttat tcctttcaat
    20701 caattactgg cgattcactg taagcgtggt ctggtatggg gcgagctgga atttgtactg
    20761 ccggacgaaa aagtggtgcg tctgcacggc accgaatggg gcgagacgca gcgtttttac
    20821 catcatcttg atgctcactg gcggcggtgg agtggcgaga tgagcgaaat tgcgtctggt
    20881 gttttacgcc agcaactgga tttgattgcc acgcgcactg gagaaaataa atggctgacg
    20941 cgtgagcaaa cctctggcgt tcagcaacaa atccgccagg ctttgtcggc gttgccgttg
    21001 ccggttaacc gactggaaga attcgataac tgccgtgagg cgtggcgtaa atgtcaggcc
    21061 tggttgaaag atattgaaag cgctcggttg cagcataacc aggcgtatac cgaagccatg
    21121 cttaccgagt atgcggattt tttccgccag gtcgagtctt caccgctgaa tccggcgcag
    21181 gcccgggcag tcgttaatgg cgagcattct ctgttagtgc tggcaggtgc aggaagcgga
    21241 aaaacgtcgg tgctggtggc ccgtgcaggc tggttgctgg cacgtggtga agcgtcccct
    21301 gagcaaattt tattgctggc gtttggtcgc aaagccgctg aagagatgga cgagcggatt
    21361 cgcgaacggc tacataccga agacattacc gcacgcacgt ttcatgcgct ggcgctgcat
    21421 attattcagc agggcagcaa aaaagttccg atagtcagca aactggaaaa tgataccgct
    21481 gcccgtcatg aactctttat tgctgagtgg cgcaagcaat gcagcgaaaa gaaagcgcag
    21541 gcgaagggct ggcggcaatg gctgacggaa gaaatgcagt ggtcagtgcc agaaggtaac
    21601 ttctgggatg atgaaaaatt acagcgtcgc cttgcctctc gcctcgatcg ctgggtaagt
    21661 ctgatgcgga tgcacggtgg tgcacaggca gaaatgattg ccagtgcacc cgaagagatt
    21721 cgcgatctgt tcagtaaacg tatcaagttg atggccccgt tattaaaagc ctggaaaggt
    21781 gcgctgaagg cagaaaacgc tgtcgatttt tcgggcctta ttcatcaggc gattgtgatt
    21841 ctggagaaag gtcgctttat cagcccgtgg aagcatattc tggttgatga atttcaggat
    21901 atctcgccgc agcgggcagc gttgttagcg gcattacgca agcaaaacag tcagacgacg
    21961 ttgttcgctg ttggtgatga ctggcaggcg atttaccgat tcagcggtgc gcaaatgtcg
    22021 ctcaccaccg ctttccatga aaactttggt gaaggcgaac gttgtgattt agacacgact
    22081 taccgtttta acagtcgtat cggtaaggtg gcaaaccgtt ttattcagca gaacccaggc
    22141 cagctgaaaa agccgctaaa cagcttaacc aatggagaca aaaaagccgt cacgttattg
    22201 gatgagagtc aacttgacgc tttgctggat aagctctctg gttatgccaa accggaagag
    22261 cgcattctga tcctggcgcg ttaccatcac atgaggcctg ccagcctgga aaaagcggca
    22321 acacgctggc cgaagttgca aatcgacttt atgaccattc atgccagcaa agggcaacag
    22381 gcggattacg tcatcatcgt tggcttgcag gagggaagtg atggttttcc ggctgcggcg
    22441 cgggagtcga ttatggaaga ggcgctactg ccaccggttg aggatttccc ggacgctgaa
    22501 gaacggcggt taatgtacgt ggcgctgacc cgggcacgcc atcgggtatg ggcactgttt
    22561 aacaaagaga atccctctcc ctttgttgaa atactgaaaa atctggatgt gccggtggcg
    22621 agaaaaccgt aagaaacagg tggcgtttgc cacctgtgca atattacttc agacggtccg
    22681 cgagataacg ctgataatcg gggatcagaa tatcgaccgc gtcgttgaaa tgcggcgact
    22741 ggattatgaa gtctgccgtt gccacgttgg tggcgaccgg aatgttccat accgtcgcca
    22801 gacgcagcaa ggctttcacg tcaggatcgt gcggcacggc atttagtgga tcccagaaga
    22861 aaatcaatac atcaattttc ccttctgaga tcaatgcgcc aacctgctgg tcacccccca
    22921 ttgggccact caacatcgcg ttgacgttca tgccggtcgc gcgggaaatt aagttaccgg
    22981 tagtgcctgt tgcatacagt acgtgttgtt ccagtaacgg ttgatgccgt tccacccagc
    23041 tcatcagcat ttgtttgcag tgatcgtgtg ccaccagcgc aatatgtttc cgcgcaggta
    23101 aagtgcgagt cgtcagttcc ataatgtaca tccgtagtca actttcctac agattactgt
    23161 aagcacttat cgctgcaaga taaagaccga aaaagcctgc gcacaggcac aaaaatctca
    23221 ggaagatggt tgtttttccg cccactgcag gaaagtattt cgcgtttgtg ggtcagccag
    23281 tttaaaccaa tacttcagcc gttgttctgt gagcacctga gactgcggtg gaatagcatc
    23341 cagctcggaa acacccgaaa gcagcgtact gtcatctgcc atcatcgtgg caaattgcgg
    23401 tagtgaagcg cgtttcgcgg atttgttata gcgttcaacc tcgacctcat aatcaatcgt
    23461 ttttgccgtt gaggtaatgg cgagaatatc cagctttacc ggaatcggcg tcgcatcgcc
    23521 atccaacaat tcaaggcgcg gcgcggcatc aaaatggtta gcttcccgct cattctccag
    23581 gcgaggcagg cgaaaattga cctggttgat gagctgggta ttaaaactga ccactagcgg
    23641 tggggagata tacagccgtt cttcactatt ggaaagatga atcgtcttct caacgcgaaa
    23701 cactaactga tgcggtccat tatccagttc aatgctgtcc gcaccgcgca gcagagagct
    23761 ggaaaccttc ttgccgtcga gtaccagaag atcgacatcg gttgaaagcc gcagcgtggt
    23821 ggcaaaaacg gataccggca aacataatgc aatcaaggtg gtcacgatgc cggttttcat
    23881 aggtagctcc tgtcaaaaga ccgcccgaat aataatgtgt caaaattttt ctttaatcat
    23941 acttactaac atatagacat atttcccgct tttgctctca ttcattcgta ttagctgcat
    24001 ggttggcatg tcggctttgt cgtacacttt gttaaacagt ctggaggaaa taatgaaaga
    24061 aaccgatatt gcaggcattt taacgtccac tcacaccatt gcgctggtgg gggcaagcga
    24121 caaacccgat cgtcccagct atcgcgtgat gaaatatcta ctcgaccagg ggtatcatgt
    24181 cattccggtt tcgccaaaag ttgccggcaa aacgctgctg ggccagcagg ggtatggcac
    24241 gctggcagac gttccggaaa aggtcgatat ggtcgatgtt ttccgtaatt cagaagcggc
    24301 gtggggcgtg gcacaggaag ctattgccat tggcgcgaaa accctctgga tgcaactggg
    24361 ggtgattaat gagcaagcgg cagtactggc acgggatgcc gggttgaatg tggtgatgga
    24421 tcgctgcccg gctattgaga tccctcgcct gggcctggcc aaataaaaaa tccccggaag
    24481 gcaaaaacct tccggggatt cgttcaggga tcagttacgc agacgcgggg cctggagttg
    24541 tttgcggatg gtctgcgcca gctcatccat ggatggctgt tcaggatgtt catcctgcaa
    24601 ttcgctactt agctgggctt cagccaggta ggtatgaacc ggtaggccgt tatcgtcctc
    24661 catcactacg tgataccacg gagcggcgcg aagctcgtca tttaccgcca gctcatcagg
    24721 cgacggttca gagagcgaat aaaccgggtc gatatccacg accactccga gataacctaa
    24781 cagggaatgg cggacctgct ggccgatacc gaatttgctg gcaatcatag tcacctcccg
    24841 ggaaatcttt acctttgata taagggtaaa attccacatt tcaagttaca tgacgcgaca
    24901 ggcaaaccct ttcagatata gcccttccgg ataggtagcg atcaccggat gatcggctgc
    24961 ctgacggaac tgctctataa attgtacatc acggccggca tcaattgcgg catccgcgat
    25021 gattttctga aataaatcgc tggtcatcag accggaacag gagaaagtca ggagaatacc
    25081 gccttcattc agcaactgaa tcgccagcat gttgatatct ttatagccac gacacgcgcc
    25141 catcaactgg cttttattct caacaaactt cggcgggtcc atcacgataa cgtcaaattt
    25201 ttcaccgcga tcgcgataag tacgcagcaa tttaaagaca tcatcacgga caaactcagc
    25261 cttgctcaga tccagtttgt tcagctcaac gttctgccgt gcaatatcca gcgcttcctg
    25321 ggaggtatca acgctgacaa cctggctgca accgcccatc agtgccgata cggcgaaacc
    25381 accggtatag gagaaacagt tcagcacacg tttattttca acgtagcggc gggtagccag
    25441 gcggctgtct cgctggtcca ggtagtagcc cgttttgtgt ccgtgctgaa tatccaccag
    25501 cagcttcatg ccgtgttctt caatcggcag cagggcaggc ggcaactcgc cggtgacgag
    25561 gcccagggtc agctccatcc cttctttttt acgtaccgca acatcgctgc gatcgtaaat
    25621 cgcacattcc gggtacagcg tttgcagggc actaactaat gccgcgcgct gatattctgc
    25681 cccagcactc agcagttgca gcaccagaaa attaccgaaa cgatcgatag taatacccgg
    25741 caggccatca gattctccgg cgattaaacg atagctgtcg aggccatctt tttgcgccag
    25801 ccagtcacgc catttttgtg cttgttgcaa acggcgggaa aaaaaagcaa tgtcgataga
    25861 ctcagacggg tcaaacgtcc agacgcgcgc ccggatttgc gaagctggcg aataagcgcc
    25921 gcgtgctaac cattttccct gatgatcaac aatatcgatg gtttcaccga ggctggcttt
    25981 accttccatg cgggcaacgg ccccggaaaa gacccacgga tggcgacgaa gtaatgattt
    26041 ttcgcgccct ttggctaaca ctaaacgtac actcataatt tacttttctg tcgatgcaaa
    26101 gaaatgggcg tcattgtccg gagttttcca tggatttgca acgcgtccat taaggataag
    26161 gagagcagca atgtcgaaag tctgcataat tgcctgggtt tacgggcgag ttcagggcgt
    26221 aggatttcgc tacaccacac agtacgaagc gaaaagactg ggtttaaccg ggtacgccaa
    26281 aaatcttgac gatggcagcg tggaagtggt tgcctgcggt gaagaagggc aggtggaaaa
    26341 attaatgcag tggttaaaga gtggcggccc gcgttctgcg cgtgtagaac gggtgctcag
    26401 cgagccgcat catccctcgg gggaattaac cgattttcgt atccgttaaa tacattttac
    26461 cggttttggc aggccagcaa ttttggtggc ttgcttagcc ggacctttcg gaaacagtcg
    26521 gtataaatag cggctgttac ctttttcttc gccaaattta ttcgccatcg cttttaccag
    26581 catacgaatc gccggagaag tattgaattc cagatagaaa tcacgcacaa aacgcaccac
    26641 ttcccagtgt tccggtgaca gcgaaatccc ttcgttctct gcaatcacca ctgccagcgg
    26701 ctcactccac tggctgcttt ctttgagata gccttcggta tccgtttcta tctctttacc
    26761 ttcgaagatc agcataatta ttactacctt aatcagactg gcggcagtgt aacaaaaaac
    26821 aaagccccgc ataaagcgag gctatgaaag tgttagcggg tgagattaat cgcggctggc
    26881 gaagcccaga atgctcagca ggctgacgaa gatgttgtac agcgaaacat acaggctaac
    26941 cgtggcacga atatagttcg tttcaccgcc atgaacgatg ttgctggttt caaacaaaat
    27001 agcgccagag gagatcaaaa tgaagaccgc actgatcgcc agatgcagag caggcagctg
    27061 caggaagata ttagcaacca taccaatcag caccaccaca atacccgcca tcagcatacc
    27121 gccgaggaac gacatatctt tgcgggtggt cagcacatat gcagagcagc agaagaacac
    27181 taacgccgtt ccgcccagag ccatagcgat tacgtccccc attccggcag acagataggt
    27241 gttcagaata ggtccgagga tataacccag aaaaccggta aaggcgaatg cggagataat
    27301 cccggtcggc ttattcgccg ttttataggt caggaacatc aaaccataca tacccaccag
    27361 cgtcagaatc agacccggag atggcagcat cagcaccgta ctggcagttg cggtaatcgc
    27421 cgaaaaggcc agcgtcaggc tcagcagaaa ataggtatta cgcagcacct tatgggtgct
    27481 aagcagtgat gtacggtcat gtgaagaact aacaatacga tccattgagt cactctctat
    27541 gacagatgta attaattaag cagcataatg ataatgcgta agggcaccca gaagttttac
    27601 ccatctttac gcatttgatc tggaacaggt ttaacagcgg attatcaggt cattaagcaa
    27661 atataacgcc ctgagaattt cgacaggcaa aagaaaaagg ggttagcatt tagctaaccc
    27721 cttatcttat ttggcggaag cgcagagatt cgaactctgg aaccctttcg ggtcgccggt
    27781 tttcaagacc ggtgccttca accgctcggc cacacttccg gaatgacgcg cactataaac
    27841 atcccgatgc ggcgtgtaaa cccctaattt gtttgtttgc ctgaaaaaca gccaaaagtg
    27901 cattgatagc gtgaaataac agcagattga tcatttcatc accatgaatt ccttctcttt
    27961 tactcgttta gcaaccggct aaacatcccc accgcccggc aaaaagaaaa ataggtccat
    28021 ttttatcgct aaaagataaa tccacacagt ttgtattgtt ttgtgcaaaa gtttcactac
    28081 gctttattaa caatactttc tggcgacgtg cgccagtgca gaaggatgag ctttcgtttt
    28141 cagcatctca cgtgaagcga tggtttgcct tgctacaggg acgtcgcttg ccgaccataa
    28201 gcgcccggtg tcctgccggt gtcgcaagga gaagagacgt gcgatatgaa taacgaggaa
    28261 acattttacc aggccatgcg gcgtcagggc gttacccggc gcagctttct caaatattgt
    28321 agtctggctg ccacgtcgct gggattaggc gcgggaatgg caccaaagat tgcctgggcg
    28381 ctggagaaca aaccccgcat tccggtggta tggatccacg gtctggaatg cacctgctgt
    28441 accgaatctt ttatccgctc cgctcaccca ctggcgaagg acgtcatcct ttccctgatt
    28501 tccctcgatt atgacgatac tttgatggct gccgccggaa cccaggcgga agaagtcttc
    28561 gaagacatca tcacgcaata caatggcaaa tatatcctcg cagtagaagg taatccgccg
    28621 ctgggcgagc aggggatgtt ctgtatcagc agcggtcgac cgtttattga gaaactcaaa
    28681 cgtgccgctg ccggagccag cgcgattatc gcctggggaa cctgcgcgtc ctggggctgc
    28741 gtgcaggccg cgcggcccaa tccgacgcag gcaacgccta tcgacaaagt catcaccgac
    28801 aaacccatta tcaaagtacc tggctgcccg ccgatcccgg atgtgatgag cgccatcatt
    28861 acttacatgg tgacctttga tcgcttgcca gatgtcgaca gaatgggccg tccgctgatg
    28921 ttctatggtc agcgaatcca cgataaatgc tatcgccgcg cccacttcga cgccggagag
    28981 ttcgtccaga gttgggatga tgacgctgcc cgcaaaggtt actgcctgta caaaatgggc
    29041 tgcaaagggc ctaccaccta taacgcctgt tcctccacac gctggaatga tggcgtttct
    29101 ttcccaatcc agtctggtca cggctgcctg ggctgtgcgg aaaatggttt ctgggatcgc
    29161 ggttcgttct acagccgcgt ggtcgatatt ccgcaaatgg gtactcattc caccgccgat
    29221 accgtcggtt taaccgcgct tggcgtggtg gcagcggctg ttggtgtgca cgcagtcgcc
    29281 agcgccgttg accagcgcag acgtcataac cagcaaccta cagaaaccga acatcagcca
    29341 ggcaatgagg ataaacaggc atgagcactc agtacgaaac tcagggatac accatcaata
    29401 atgccggacg ccgcctggtg gtcgacccga ttacgcgcat cgaaggccac atgcgctgcg
    29461 aagtgaatat taacgatcag aatgtgatca ccaatgccgt ctcctgcggc accatgtttc
    29521 gcgggctgga gatcatccta caagggcgcg acccgcgcga tgcgtgggcg ttcgttgaac
    29581 gtatctgcgg cgtctgtact ggcgtacacg ccctggcttc ggtttacgcc atcgaagatg
    29641 ctatcggtat taaagtgccg gacaacgcca atatcatccg caacattatg ctggcaacgc
    29701 tctggtgcca cgatcatctg gtgcacttct atcagcttgc cgggatggac tggatcgatg
    29761 tgttagatgc gctgaaagcc gacccgcgga aaacctccga actggcgcaa agtctctcct
    29821 cttggccgaa atcatcccct ggctatttct tcgacgtaca aaaccgcctg aaaaaatttg
    29881 ttgaaggcgg gcagttgggg atcttccgca atggctactg ggggcacccg cagtacaaac
    29941 tgccgccaga agctaacctg atgggctttg cccactatct cgaagctctc gatttccagc
    30001 gtgaaattgt caaaatccac gcggtctttg gcggtaaaaa cccgcatcca aactggattg
    30061 tcggcgggat gccttgcgcc atcaacattg acgaaagcgg cgcggtcggg gcagtcaata
    30121 tggaacgcct gaacctggtg cagtcaatta tcacccgcac ggcggacttc attaacaacg
    30181 tgatgatccc cgacgcctta gccatcggtc agttcaacaa accgtggagc gaaatcggca
    30241 ctggtctttc tgataaatgc gttctcagct acggcgcatt cccggatatt gccaacgact
    30301 ttggcgagaa aagtctgctg atgcctggcg gcgcggtgat taacggcgac ttcaacaatg
    30361 tgctgccagt ggatttggtt gatccgcagc aggtgcagga gtttgtcgac cacgcctggt
    30421 atcgatatcc caacgatcag gtcgggcgtc atccgttcga tggcatcacc gacccgtggt
    30481 acaaccccgg cgatgtcaaa ggcagcgata ccaacattca gcagctgaat gaacaggaac
    30541 gctactcgtg gatcaaagcg ccacgctggc gcggtaacgc gatggaagtg gggccgctgg
    30601 cgcgcacgtt aatcgcttat cacaaaggcg atgctgcgac cgttgagtcg gtcgatcgca
    30661 tgatgtcggc gttgaacctg ccgctttccg gtatccagtc aacgttaggc cgcattttgt
    30721 gccgcgcgca cgaagcgcag tgggccgcag gtaagttgca gtatttcttc gacaagctga
    30781 tgaccaacct gaaaaacggc aatctcgcca ctgcttccac ggaaaaatgg gaacctgcaa
    30841 cctagccgac agagtgccgt ggtgtcggtt ttaccgaagc gccgcgcggg gcgttaggcc
    30901 actgggccgc cattcgcgat ggcaagattg atctctacca gtgcgtggtg ccgaccacct
    30961 ggaacgccag cccgcgcgat cccaaagggc agattggcgc ttatgaagcg gcgctgatga
    31021 acaccaaaat ggcgatcccc gagcaaccgc tggagatcct gcgtactctg cacagctttg
    31081 acccgtgcct cgcctgttca acacacgtgc tgggcgacga cggtagcgag ctgatctccg
    31141 tgcaggtgcg ttaacagcga aggagaatca tcatgcaaca gaaaagcgac aacgttgtca
    31201 gccactatgt ctttgaagcg ccagtccgca tctggcactg gttgacggtg ttatgcatgg
    31261 cggtgttgat ggtcaccgga tactttatcg gcaagccgct accttccgtc agcggcgagg
    31321 cgacgtatct gttctatatg ggctacatca ggttaattca cttcagcgcc gggatggttt
    31381 ttaccgtggt tttgctgatg cggatctact gggcttttgt tggcaatcga tactcccgcg
    31441 agctgtttat cgtgccggta tggcgtaaaa gctggtggca gggcgtgtgg tatgaaatcc
    31501 gctggtatct gtttctggca aaacgtccga gtgccgatat aggccataat cccatcgccc
    31561 aggcggcgat gttcggctat ttcctgatgt cggtctttat gatcatcact ggttttgcgc
    31621 tgtacagcga acacagccag tacgctattt ttgcgccgtt ccgttatgtg gtggaatttt
    31681 tctactggac gggtggcaac tcaatggaca ttcacagctg gcatcggctg gggatgtggc
    31741 tgattggcgc gtttgtgatc ggtcatgtct acatggcgct gcgtgaagac atcatgtccg
    31801 acggcacggt gatctccacc atggtcaacg gctaccgtag ccacaaattt ggcaaaataa
    31861 gtaacaagga gtgttcatga gcgagcaacg cgtggtggtc atggggctgg gcaacctgct
    31921 gtgggccgat gaaggcttcg gcgtgcgggt ggcggaacgg ctgtatgccc attaccactg
    31981 gcccgagtat gtggagattg tcgatggcgg tactcaggga ctgaacttgc tggggtatgt
    32041 cgaaagcgcc agccatctgt tgattctcga tgccattgac tacgggctgg aacctggaac
    32101 gctgcgaacc tatgccggag aacgcattcc ggcttatctc agcgcgaaga aaatgagcct
    32161 gcatcagaac agtttctccg aagtgctggc gctggcagat atccgcggac atctgccagc
    32221 acatattgcc ctcgtcgggc tgcaacccgc gatgctcgac gactacggcg gcagcctgag
    32281 cgaactggca cgggagcaac tgctcgctgc ggaacaatcg gcgctggcgc agttggctgc
    32341 gtggggaagt gtgccgcaac tggctaatga atcgcgttgt ctcaattatg actgtctgtc
    32401 gatggaaaat tacgaaggcg ttcgcttgcg ccagtaccgg atgacacagg aggagcaggg
    32461 atgagcaacg acacgccatt tgatgcgttg tggcaacgaa tgctggcgcg cggctggacg
    32521 ccggtcagtg aatcccgtct tgacgactgg cttacgcaag cgccagacgg cgtggtgtta
    32581 ttaagcagtg acccgaaacg cacgccagag gtcagcgata atccggtaat gattggcgaa
    32641 ttactgcacg agtttcccga ctatacatgg caggtggcga ttgctgacct tgagcagagc
    32701 gaagccatcg gcgatcgctt tggcgtcttt cgctttccta ccactttagt gtttaccggc
    32761 ggaaactatc gcggcgtgct gaatggtatt cacccgtggg cggaactgat aaacctgatg
    32821 cgcgggcttg tcgaaccgca gcaggagcgt gcctcatgag cgaaactttt ttccatctgc
    32881 tggggccagg aacgcaaccg aacgatgaca gtttcagcat gaatccactg ccgatcacct
    32941 gtcaggtgaa tgatgaaccg agtatggcgg ccctggagca atgtgctcac agcccgcagg
    33001 tgattgcgct gttaaacgag ttacaacatc aactaagcga acgccagccg ccgttgggcg
    33061 aggtgctggc agtcgatctg ttaaatctca acgccgacga tcgtcacttt atcaatacgc
    33121 ttctcgggga aggggaagtg tcagtgcgca ttcagcaggc tgacgacagt aaaagtgaaa
    33181 tacaggaggc gatcttctgc ggattatggc gggtgcgcag acgtcgcggc gaaaagttgc
    33241 tggaggacaa actggaggct ggctgcgcgc cgctggcgtt gtggcaggcg gcaacgcaaa
    33301 atctcttgcc gacagattcg ctgttaccgc cgcccattga tggcctgatg aatggcctac
    33361 cgttggcgca tgagttactg gcacatgtac gtaaccccga cgcgcagccg cacagcatta
    33421 atctgacgca attacccatc agcgaggctg atcggctttt tctctcacgt ctctgtgggc
    33481 cgggaaatat tcagattcgt accattggct atggcgagag ctatatcaac gccacggggt
    33541 tacgccatgt ctggcattta cgctgtacgg acaccttaaa aggcccgtta ctggaaagtt
    33601 atgaaatctg cccaataccg gaagtggtgc tggcagcgcc agaagatttg gtcgactctg
    33661 cgcagcggct tagcgaggta tgtcagtggc tggcggaagc tgcaccgacg taaaaagacg
    33721 gtaagtatcg ctttcagtct tatgaatatc gcaatcggcg aatacctctg gtcgtagagt
    33781 ttcaggataa agagggagat ctaacattat cgggttattt ttctctcttc gcctacagga
    33841 gtgcgcatgt gggatgtcat tgatttatcg cgctggcagt ttgctctgac cgcgctgtat
    33901 cactttttat ttgtacccct taccctgggg ctgatttttt tgctggctat catggaaacc
    33961 atttacgtgg tcaccggcaa aacaatctac cgcgatatga cgcgcttctg gggtaagctc
    34021 ttcggtatca attttgctct tggcgtggct accggcctga ccatggagtt tcagtttggt
    34081 actaactggg cattctattc caactatgtg ggcgatattt ttggcgcacc gctggcgatg
    34141 gaagcattaa tggccttctt cctcgaatcc acctttgtcg ggcttttctt cttcggctgg
    34201 cagcggctga ataaatacca gcacctgctg gtgacgtggc tggtggcgtt cggttcaaat
    34261 ctctctgcat tgtggatatt gaatgccaac ggttggatgc aatacccgac cggtgcgcat
    34321 tttgatatcg acaccctacg tatggagatg accagcttca gcgagctggt ctttaacccg
    34381 gtcagccagg tgaaatttgt gcacaccgta atggcgggct acgtgaccgg ggccatgttt
    34441 attatggcga tcagcgcctg gtatttactg cgcggacggg agcgcgatgt tgcattacgc
    34501 tcgtttgcca tcggttccgt cttcggtact ctggcgatta tcggcacgct acaactcggg
    34561 gacagttctg cgtatgaagt cgcgcaagta caaccggtaa aactggcggc gatggaaggg
    34621 gagtggcaaa cggaacctgc tcctgcaccg ttccatgtgg ttgcctggcc ggaacaggat
    34681 caagagcgta acgcctttgc catcaaaatt cccgcgctgt tagggatcct cgccactcac
    34741 tcattagata aacccgtgcc gggtctgaag aatttgatgg ctgaaaccta cccacgcttg
    34801 caacgcggac gtatggcctg gctgttaatg caggaaatat cgcaaggcaa tcgtgagccg
    34861 catgtgttgc aggcattccg ggaactggaa ggtgacctgg gctacggcat gttgctctcc
    34921 cgctatgcgc cggatatgaa tcatgtcaca gccgcacagt accaggcggc gatgcgtggc
    34981 gcgatacctc aggttgcgcc ggtattctgg agtttccgca tcatggtggg ctgtggttcc
    35041 ctgctgctac tggtgatgct gattgcgctt gtccagacgc tgcgtggcaa aatcgaccag
    35101 catcgctggg tgctgaaaat ggcgctctgg agtttgccgt tgccgtggat tgcgattgaa
    35161 gccgggtggt ttatgaccga gtttggtcgt cagccgtggg cgatacagga catcttaccg
    35221 acatactccg cgcactccgc tttaaccaca ggacaactgg ctttctcact gatcatgatc
    35281 gtagggcttt acaccctgtt cttaatcgcc gaagtctacc tgatgcagaa atatgcccgt
    35341 ctggggccga gcgcgatgca gagtgaacaa ccgacgcagc aacaggggta aagaagaaaa
    35401 tcatgtttga ttatgaaaca ttgcgcttca tctggtggct gctgattggc gtgatcctgg
    35461 tggtctttat gatctccgac ggatttgaca tggggatcgg ctgtctgctg ccgctggtgg
    35521 cgcgtaatga tgatgaacgc cggatagtga taaacagcgt tggtgcacac tgggaaggca
    35581 accaggtctg gttgatcctc gctggtgggg cattatttgc cgcctggccc agagtgtatg
    35641 cagcggcgtt ttccggcttt tatgtggcga tgatcctggt gctgtgctca ctgttcttcc
    35701 gcccgctggc ctttgattat cgcggaaaaa tcgccgatgc acgctggcgt aaaatgtggg
    35761 acgccggtct ggtcatcggc agtctggtgc cgccggtagt cttcggtatc gccttcggca
    35821 acttgttgct cggcgtgccg tttgccttca caccgcaatt acgcgtggag tatctcggca
    35881 gcttctggca actgctgacg ccattccctt tattgtgcgg attgctcagc cttgggatgg
    35941 taattttgca aggtggcgtc tggttacaac tgaaaactgt tggtgtgatt catctgagtt
    36001 cacagctggc caccaaacgc gctgcactgt tggtgatgct gtgctttttg ctggcgggtt
    36061 actggctgtg ggtcggtatt gatggctttg tactgctcgc ccaggatgct aacggtcctt
    36121 ccaatccgtt aatgaaactg gtggcagtgc tacctggtgc ctggatgaat aatttcgtcg
    36181 aatcgcccgt tttgtggatc ttcccgctgc tggggttctt ctgcccattg ctgacggtga
    36241 tggcgattta tcgtggtcgc ccaggttggg gatttttaat ggcgtcattg atgcaatttg
    36301 gcgtgatttt cacggcaggc atcacgctgt tccccttcgt catgccgtca agcgtgagtc
    36361 cgatctccag cctgacgttg tgggacagca cttccagcca gctgacgctg agcattatgt
    36421 tggtaatcgt gctgatattt ttgcccattg tgttgctcta cactctctgg agctactaca
    36481 aaatgtgggg gcgcatgaca acagaaactc tccgccgtaa cgaaaacgag ttgtactaag
    36541 gagcaggaac aatgtggtat ttactttggt tcgtcggcat tttgttgatg tgttcgctct
    36601 ccacccttgt gttggtatgg ctggacccgc gtctgaaaag ttaacgaacg taggcctgat
    36661 gcggcgcatt agcatcgcat caggcaatca ataatgtcag atatgaaaag cggaaacata
    36721 tcgatgaaag cgatcttaat cccattttta tctcttctga ttccgttaac ctcgcaatct
    36781 gcattcgctc agagtgagcc ggagctgaag ctggaaagtg tggtgattgt cagtcgtcat
    36841 ggtgtgcgtg ctccaaccaa ggccacgcaa ctgatgcagg atgtcacccc agacgcatgg
    36901 ccaacctggc cggtaaaact gggttggctg acaccgcgcg gtggtgagct aatcgcctat
    36961 ttcggacatt accaacgcca gcgtctggtg gccgacggat tgctggcaaa aaagggctgt
    37021 ccgcagcctg gtcaggtcgc gattattgct gatgtcgacg agcgtacccg taaaacaggc
    37081 gaagccttcg ccgccgggct ggcacctgac tgtgcaataa ccgtacatac ccaggcagat
    37141 acgtccagtc ccgatccgtt atttaatcct ctaaaaactg gcgtttgcca actggataat
    37201 gcgaacgtga ctgacgcgat cctcagcagg gcaggagggt caattgctga ctttaccggg
    37261 catcggcaaa cggcgtttcg cgaactggaa cgggtgctta attttccgca atcaaacttg
    37321 tgccttaaac gtgagaaaca ggacgaaagc tgttcattaa cgcaggcatt accatcggaa
    37381 ctcaaggtga gcgccgacaa tgtctcatta accggtgcgg taagcctcgc atcaatgctg
    37441 acggagatat ttctcctgca acaagcacag ggaatgccag agccggggtg gggaaggatc
    37501 accgattcac accagtggaa caccttgcta agtttgcata acgcgcaatt ttatttgcta
    37561 caacgcacgc cagaggttgc ccgcagccgc gccaccccgt tattggattt gatcatggca
    37621 gcgttgacgc cccatccacc gcaaaaacag gcgtatggtg tgacattacc cacttcagta
    37681 ctgtttattg ccggacacga tactaatctg gcaaatctcg gcggcgcact ggagctcaac
    37741 tggacgcttc ccggtcagcc ggataacacg ccgccaggtg gtgaactggt gtttgaacgc
    37801 tggcgtcggc taagcgataa cagccagtgg attcaggttt cgctggtctt ccagacttta
    37861 cagcagatgc gtgataaaac gccgctgtca ttaaatacgc cgcccggaga ggtgaaactg
    37921 accctggcag gatgtgaaga gcgaaatgcg cagggcatgt gttcgttggc aggttttacg
    37981 caaatcgtga atgaagcacg cataccggcg tgcagtttgt aatgcataaa aaagagcatt
    38041 cagttacctg aatgctctga ggctgatgac aaacgaagaa ctgtctaatg cgtagaccgg
    38101 aaaaggcgtt cacgccgcat ccggccactt tcagttttac tctttctcgg agtaactata
    38161 accgtaatag ttatagccgt aactgtaagc ggtgctggcg cgtttaatca caccattgag
    38221 gatagcgcct ttaatattga cgcctgcctg ttccagacgc tgcattgaca aactcacctc
    38281 tttggcggtg ttcaagccaa aacgcgcaac cagcaggctg gtgccaacag aacgccccac
    38341 gaccgcggca tcactcaccg ccagcatcgg cggcgtatcg acaatcacca gatcgtaatg
    38401 gtcgttcgcc cattccagta attgacgcat ccgatcgcgc atcagcagtt cagacgggtt
    38461 aggtggcacc tgaccgcgag taatcacatc aaagcctcct ttgccaaaat gctggatcac
    38521 tttgttgagc tcatctttac ctgccagata ttccgacaag ccatgttcat tactcacggt
    38581 aaacaggtta tgcgaataac cacggcgtaa gtcggcatca ataaataaca ctttttgatc
    38641 ggactgggcg atcaccgctg ccagagttga actgacaaac gttttaccac tgtctggcgt
    38701 cgcaccggtg atcatcagaa tgttattctc cgtctccatc atagcgaagt gcagactggt
    38761 tcgtagcgca cgtacggctt ccacagcaga atccgccggg ttatccaccg ccaggaaggg
    38821 gatattttta gtacgatggc gctgctgatt agaaaataaa tttttcttac gcagacgggt
    38881 acgtttatcc agccactcgg acattgggat agtggcataa acgctgatgc cgtgctcttc
    38941 cagttgttcc ggggcttcta caccacgacg caacatcgca cgcgccagca cggcacccac
    39001 cgaaataaac aggccaagaa taaaaccaag caccacgttc aacgcttttt tcggtttcac
    39061 tggctgcggc tgagtgactg ctgggtcgat aatccgcacg ttaccaatgg cactggattt
    39121 cgaaatactc aactcctgct ggcggttaag taattgcaga tataccgcac ggcccgcttc
    39181 tacgtcacgg cttaaacgta acacttcctg ttgagtggaa ggcattgccg atacccgctt
    39241 attcaggcgt ttgcgttctt gctccagcgt ctggcgtttt tccagcagcg cacgataagt
    39301 tgggtgatct ttcttataca gctgggagat ctctgcctcg cggaaagtca gctcattgag
    39361 ttgattatca acgttcacaa tctgctcaag aacggctttg gcttctaggt taaggtcaac
    39421 cgaatcgcgc tgctggcgat aaacgttgag tttttcttcc gcctggtcca gctcgctgcg
    39481 cacttcaggc aactggcgct gtaagaattc aaggctttgt gaatcctgcg ccgcctggcg
    39541 agcgatattc tgttgcaaat agttgttagc gatgctgttc agaatacgag taatcaactg
    39601 gggatcatca ccagtcatgg taagttccag catcccgctt tctttactgc gttcgctaac
    39661 ggtaaaggtt tcctgcaatg cgttaatcgc ttccagttcg gtacgctggc tcaggacaaa
    39721 ctgtgttcct ggtttggcct taatgtccgc gatagtcagc gcaacgccat ctttttccag
    39781 acgctggccg accataccat tgacggtgaa ctcttcacct tccagtgtat agtggccgtt
    39841 ttccccaacc gtgagtgtca gttgctgatc ctgaccattc agttgtggaa tatgcatcca
    39901 gctgatcgcc agctcacctg gtttttcttt ggttaatctc gcccagccgc gacccacaat
    39961 cggaaaatac ttctgctcaa ctatgtcgcg cagattcagt tcagcaatgg ttttaccgag
    40021 aatcatgcgc gattgcagca gttggatctc cggtgcagac tcgggcgatg agttagggat
    40081 catatcgctc aggccgctga gaatggcgtt gccctgtttt tgctcaacct ggaccagagt
    40141 atctgcctga taaattggtg tgcttaacag cgagtaagcg acagcgatca gcgtgaataa
    40201 cgcggttacg ctgataataa acttacggtg atcccataac tcgccgacca gacgaagcag
    40261 atcgatctca ttttcctgag tgctgcctgg tggcgtattc atatttttag ttgtcatcgt
    40321 attttataaa ctcaaattac cggctgaggc gcttcgccca ttcctgactg gcgcgctcca
    40381 acataccgta gacatgttca aatgcgtcct gacttttacg atagggatcc gggatctctt
    40441 tctgttccag ccattgccca aacagcattg tcttcccgcg gacctcggga gcgattgcgg
    40501 taacctgagc aatatgctct gactccattg ccagaatcag atcgtaattt cgtgccatct
    40561 cagcagtgag cttacgtccg gcatgtcctt caagagatac gccatgatta gcggcaacat
    40621 ctgctgctgt cgcatctgca gggtgtttta ccagaccatg aacacctgcc gatttgactt
    40681 ttacgcctgg cagacgttta cgcagcaagc gctcgccaat gggcgaacgg caaatgttgc
    40741 cggtacaaac caccaggatg gagttaaatt ttagttgggc catgtttgaa tccaccgtga
    40801 agtctctgtc aggtcatgca ccccggaaat cgtcggtacc agcagagaaa tcacacggtt
    40861 ccaacgagcc agcggcgcgg tagtgacata aacaatgtcg taaggttcaa gctggaattc
    40921 cgttccgagt atcattgccg atgcgtcctt cgcattcagc tgataaatat tggcgatttt
    40981 gccattctgc ttgttttgcg ttgcacgaat aacaaaaatc cccgtcgcat cagcgacatc
    41041 ctgattcata ccctcagcgt tacccagcgc ttccgccagg gtcataccgc tgcggtccat
    41101 tttcagcgtg ctctgtttac ccacttcacc catcacgaag actttcagat catcgttgcg
    41161 cggaataaat aaaatatcgc ccgggtgcag cagtttgttc tggcgcaagt cgccacgctg
    41221 catcagcgca tagaggttca ctttggtctt tacgccattc tgggtgagca ccacgttgcg
    41281 ccagtctgca tctgcggtca gaccgcccgc ggcgttaatc gcatccatga tggtcagagg
    41341 aatattggta atcggctgct gacccgattt gctgacttcg ccggtgacgt acgctttttg
    41401 cgatcggaac gccgcgacgc tgacatcgac ttgcgggctt tcaatgactg aatcgagacg
    41461 cgctgtaatc tcgttgcgta cttgggtaag tgttttcccg gccactttca ggcgaccaat
    41521 ataggggtag aaaatcgcac catcggcgtt aacccagttg ccggtatcac tggcgctacg
    41581 gtattgccct gcgggggtgg ttagttccgg gtgatcccag accgtaacca tcaacacatc
    41641 accaatcccg atacgatatt catagtttgc gatttgctgc tccagctccg ggttagcctg
    41701 cgacataatc ggtttggcgc gcaactgatc aatcaatcct ggcgtgacgg gatagatatt
    41761 gaccattttg tccaggtcat actgattgtc aggtagttca atgacatcct tgttcctggt
    41821 cgataaattc tgaccaggaa caagggtaca tgctgtcaaa gacagaacag ccaatgtcag
    41881 taccgaaaac ttaaaaatgt tctttttcat tggtttatat ttatcagtca atgtaatgtt
    41941 atgaattacg tcaggttatt gtccaaccgg tacttctcgc tctgccgtca ttggatagag
    42001 ctggtatttg cgtgacagca tttgtccacc atcacgcgtc aatggtgtcc acgaaaccac
    42061 cgcgcggttg cggttaggtc caatggtcat caagtcgaat ggaatcgaga tataaaaacc
    42121 tttactaaag ccgccttcgc cgtagtcatc tttagatacg ttactgattg ccgcccatac
    42181 ccctaccgcc acgccgctgt caaagcgttt ggcgacgtcg atcgttgctc ctttatcttt
    42241 tgccagatat tgcccaacgc taagtttcat cagtacgcca ttgagcgtcg gcgggttcca
    42301 gtaagccgtc acgaaaccag ttggcgtgga ataatcggtg aagcgcatca tgttgtccca
    42361 gtcacgttgc ttcacgtagt taacgtccac acccagcgcc cagctggcat ctagcgggcg
    42421 ataaagcagc tcggaaccga caccggcgta catcgtttcc agataaccgc catacacctg
    42481 gccatagaaa ccattgccta agtcggcaaa gtagttcgcc tgcaagttgt tgagataaac
    42541 gtcattgcga acgtaatcac ggatatgcgt gcgcacgcgg ggcagggtag agtccgcggg
    42601 caacagcgaa gacttaaact tgtcgtagtt gttgtaaata ttggtgaaaa taccgccatc
    42661 aagcagcagg tggtcggtaa accagtagcg ggcactggac atcagcccca gctggaacat
    42721 atagaaatct tccgggccgc ccagcgactg tgaaagtgtt gggttgaaag agtagctaaa
    42781 gcgatcttca cgaatacggt agccccgacc aaaggcagaa agatcttctg cttcaacacg
    42841 ttgttgttga agttgctctg attgaccagg cgctgtacca gccagctgtt tgcgcaggct
    42901 ggctacgtcg gtttcggtag tcaccatcgc catatgctcg cgcttttgcg tcacgctaat
    42961 cttctcaacg ccttgcggca ggttattcac cagaatccgg ttggcacgat cgaccgcttc
    43021 gcgagaatca cggtatttgt attgttgacc agacatatac agcgtcttat cgcgcagctg
    43081 aatttccggt gcttcaaaac ctgcgttgta cttcagcgcg gtaagttggt tcgctactgt
    43141 ggtgtactgc aatccttcag attcaggcgc aggttgatat gccggttttg gcgtatcgcg
    43201 cagggcaggg cgcagatcgt tgaaattggt ccgtaacgtg aagccaaaca tcaacgtgtt
    43261 accgcgttca taactcaggt tgagatctgc ccagctggca gcgcgataaa ctgcgccgac
    43321 gttgaaatgg cttgcctgag gcagtttgcc agcgaaatca ttctggtaat tgttgccatc
    43381 gtattcgagt ttcagacgca ggggattcca cggcgtttga tactcaatgc cgccaaagat
    43441 ggaagccggg ccacgaaaga tatcgctaaa gctgatatcg cccgcatcgt gagactctgc
    43501 gcgatgacaa tatttatcgt tcacccggca aaacgggttg gtaatattgc ccgcattacc
    43561 ggcgtagccc catgccatcc cgagggtgaa atcaaatggc cccgcttgct tgctggccac
    43621 cagatactca ccgtcaaaca gacccgtacc agcaatatca cgtttaccaa acgccacttg
    43681 cggtagccag taaccttctt cccacaggcg aagtttaaaa tcgaatgatt tgtctttgta
    43741 tgactgatcg ccgctgaaat cttcccactg gctatatttg cgtgtgcgca catccgtata
    43801 acgaatggtg ccttccagcc atgggaacag cgccacggag gtggagtaga accggtattg
    43861 atcgttatcc cggtagttga cgctgaattc accttccggt gcgatgcgcg catttggcat
    43921 ctgcaacaat cctgtgccgc caaagtctga ttgcgacgaa cccagcggat ccgggtaggt
    43981 taatacttca gcatgacagg cactggagac ggcaatggcc aggcagctta aaagataaga
    44041 attcttcttc atcagtcagg aacacgttgc gttaaaacag acacaatctg gtcatttaaa
    44101 tcagcatatt tttcgggcag tacatgcgca gaaaagccca accaaagctg gctgccaggc
    44161 ggtgggtcca catgtctttt attccacagg gcaacagggg cgacgacagt ctcaccctca
    44221 ggcgtaatca ccataacgtt attcttgtcc gcgcctgcaa ggcgggggtg atcttgcaga
    44281 taatcggtaa cgctacggcc tgcctgccag ggcaattgcc cggccccaga aaccgcaccc
    44341 agcagagtaa tagtcaccgg acgctgtacg gtatagagcg tatagtcccc gaccaacggg
    44401 ggattgctgt tctcatccac tcgaacaaaa tcagggtcca gtttgaccgg taagcgacca
    44461 gtgatattga gattgagtaa ctgctggcgc acagatttaa tcgttgccgc cacatcgtcg
    44521 tcagcttctg cttcccagga agcaagctgt gccatgacat gttggtagtc tttaagcgct
    44581 ttagctttcg ccgcagagtc ggtcagcaag gcaccaggcc accagaggcg atcgcgcagt
    44641 tgcggttgtg tcaccaactg gacaacattt tccatggggc caacggaaag tgtctgttgt
    44701 tcgccaggca gataaatagt caccgttcct tgcgcaaagg catggggtgt cattacgtaa
    44761 agtacgctgg caatgaaata cgactgtaat ttattcatgg tgcgggttta agaaacgtca
    44821 tttctacggg aataacccct gcgcctaaca tttgccgact ttgacgaacc tggccagtag
    44881 ccgaatcgac ccagaatgtg ttttgccact gtttttctgg ccgcgtggaa gtcacctctt
    44941 cctgccagac attacacagc acattgcgac ccgcaatatt cagcgtctct aatccagcaa
    45001 aggaaaaagt agaactcagg gtcgcagagc gaaaatggtt gtcttcactc cagagaatat
    45061 cgcgcgtcca gcggctgcca tcttttattg ccagcgcgtt acgcagaggg tcctgcccgg
    45121 aatttgtcac ttccagcaga ttattattaa gtttgacggt ttttaaaaga cgtccattgt
    45181 gggtaaccag catggcgtta tcctgggata accatttgct ttgttcttgt tctatatatc
    45241 ccagtaccac aaagattcgt tgcccttcat taaggcgtaa atacatcgtg gaataaggca
    45301 acgcctggat ctgctgatcc gctacggtga tatcctgatt atcgaaaagg ctggcgcgaa
    45361 atgtatcgac catactttgc tggctgtgcg tacatcccgc aagaaatagt gcgaaaatcg
    45421 ataaaataag agggcgcacc tttaatcctt gaaaggttaa ttaaaaaaat gcaaatcttt
    45481 aaagcccggt acgaatatcg caaccgggta tcatttaact ttaatttcga cgcgttaaag
    45541 cagtattagc gtgtagtgga cgtggtggtt gaggtgttat gagaaccgcc atcgccactg
    45601 tcatttgctg caacaactgc caacgtcgcc acaccaacag ccgttgccac accgacaact
    45661 gtcgccgtgc cgacagcgcc agcagccacg ccaccagtgg tagtagcagt cgtggttgcg
    45721 ccggtagtgg tcgttgtggt gccagtagta cccgtcgttg cctcggtccc gtttgttgct
    45781 tcaggggcag caaacgcagc tggcgtcgtc aacatgaagg ccataaggat tgcagaaagc
    45841 ttgtgtttca taacttttcc tttattcatc gcatggacaa tacgtacgct tgagatcagg
    45901 attgtctact ttgagtataa catccttgtg aaatctccct ttcctcatac cgcttgcact
    45961 atatactgcg ggtaggaaag gcgcgcagag ggaaatataa gattgttaac tatccccctc
    46021 tggaaatacg acttgtgagc ataagataaa tcttagcaaa atacaaatta aagataagga
    46081 aattgttacg aaagctatta attattgctg gtaatatagt ttcaagtggt actataactg
    46141 gctgttaaat ggcatttata actattaggt gcagagatat tcgcttaaat ggattagttt
    46201 ctttgtggaa aagtaactga tgttataata tatgatgttg actattgcac tattccaata
    46261 aaaccagtca gctttaaaca agcagcgtca tattaagaga gataaacatt tgccgctgtt
    46321 ggtcctcgca ggccatttac gcggcaaaat tccacacgta atcctggtat aagcacttct
    46381 gcgtcgcggg gagtgaatgc ggaaatatgg acctgaactt ctttacgacc gtcggagggg
    46441 ataatgaatc ctttgccgct tttgcgatca aaggttttga caattcctgt cattttacgg
    46501 gacaaacaaa ttccttactg aaaatactgc gctgcactat acggggttaa taaaataaag
    46561 ccagcgatat ttaagaccgc cggacggcta aaataaaatt tgcttaatct caattatcat
    46621 gcgttaatag ctgcgtcggt ttgaaagaca gacagcatac aaagtagttt actaaagcag
    46681 ttctcattat caggcattat ccccttcttt tgagtctctc tcctgaacac taagtagttt
    46741 ctgtattaaa gccctgtttg ccgaaaggcc caaaatgaag gaagtaaaat atgtctaata
    46801 aaatgactgg tttagtaaaa tggtttaacg cagataaagg ttttggcttt atcactcctg
    46861 atgatggcag caaagacgtt ttcgtccatt tcactgccat ccagagcaat gaattccgca
    46921 cgctgaacga aaatcagaaa gttgaatttt ctattgagca ggggcaacgt ggccccgcgg
    46981 cagcgaacgt tgttacgctc taaggttgcc gttattactc aacatctcca tttccgctgt
    47041 ccatgttgtc atggttcaca gtaccgcaca tcggcattcg atgtgacgga gcgaaatcct
    47101 ttgggcgcta agtgtatttt ttgtaaatcg acgatgatca cctttgataa cgtcacgctg
    47161 caaatacgca ctgaccatgc gccgctggat ttcacaaaat aatatcaggc tccctcg
//
LOCUS       DAZPEB010000007        43881 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZPEB010000007 DAZPEB010000000
VERSION     DAZPEB010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43881
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.007"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            85..627
                     /gene="lpfE"
                     /locus_tag="ACHURJ_000405"
     CDS             85..627
                     /gene="lpfE"
                     /locus_tag="ACHURJ_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HFR1279488.1"
                     /translation="MKRTILALCLLPLSFSSFAATTDLGPKGTLKFTLKISQGACELV
                     KDSVEVDMGTAVLKKPVRIGTEINPTPFSIGLKNCSEVVRAYVTMDGTPNADDSNLFA
                     LDAGGATGVGLKIKTAAGVQQFPENTTPIPVEFAINGDGAHQLNYIASYVPIRADATI
                     GRADATVDFTVQYSTNNFTS"
     gene            867..2558
                     /gene="eptB"
                     /locus_tag="ACHURJ_000406"
     CDS             867..2558
                     /gene="eptB"
                     /locus_tag="ACHURJ_000406"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFR1279489.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQAKEAAAN"
     gene            2650..2726
                     /locus_tag="ACHURJ_000407"
     tRNA            2650..2726
                     /locus_tag="ACHURJ_000407"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2684..2686,aa:Pro,seq:cgg)
     gene            3470..5077
                     /gene="dppA"
                     /locus_tag="ACHURJ_000408"
                     /pseudo
     CDS             3470..5077
                     /gene="dppA"
                     /locus_tag="ACHURJ_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
     gene            5385..6404
                     /gene="dppB"
                     /locus_tag="ACHURJ_000409"
     CDS             5385..6404
                     /gene="dppB"
                     /locus_tag="ACHURJ_000409"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFR1279490.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPASGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            6414..7316
                     /gene="dppC"
                     /locus_tag="ACHURJ_000410"
     CDS             6414..7316
                     /gene="dppC"
                     /locus_tag="ACHURJ_000410"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFR1279491.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDMLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDIAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            7327..8310
                     /gene="dppD"
                     /locus_tag="ACHURJ_000411"
     CDS             7327..8310
                     /gene="dppD"
                     /locus_tag="ACHURJ_000411"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFR1279492.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLVFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            8307..9311
                     /gene="dppF"
                     /locus_tag="ACHURJ_000412"
     CDS             8307..9311
                     /gene="dppF"
                     /locus_tag="ACHURJ_000412"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFR1279493.1"
                     /translation="MSTQEATLQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLSGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(9341..10612)
                     /locus_tag="ACHURJ_000413"
     CDS             complement(9341..10612)
                     /locus_tag="ACHURJ_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFR1279494.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            11088..11195
                     /locus_tag="ACHURJ_000414"
     CDS             11088..11195
                     /locus_tag="ACHURJ_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR1279495.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(11282..12961)
                     /gene="bcsG"
                     /locus_tag="ACHURJ_000415"
     CDS             complement(11282..12961)
                     /gene="bcsG"
                     /locus_tag="ACHURJ_000415"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFR1279496.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVETTGGAPVVGDMPAQTTPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTTVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(12958..13149)
                     /gene="bcsF"
                     /locus_tag="ACHURJ_000416"
     CDS             complement(12958..13149)
                     /gene="bcsF"
                     /locus_tag="ACHURJ_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFR1279497.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(13146..14717)
                     /gene="bcsE"
                     /locus_tag="ACHURJ_000417"
     CDS             complement(13146..14717)
                     /gene="bcsE"
                     /locus_tag="ACHURJ_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFR1279498.1"
                     /translation="MRDIVGPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDYQGPEKIKLFSMLNHEKGLYYLTRDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLTHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            14990..15178
                     /gene="bcsR"
                     /locus_tag="ACHURJ_000418"
     CDS             14990..15178
                     /gene="bcsR"
                     /locus_tag="ACHURJ_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFR1279499.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            15190..15942
                     /gene="bcsQ"
                     /locus_tag="ACHURJ_000419"
                     /pseudo
     CDS             15190..15942
                     /gene="bcsQ"
                     /locus_tag="ACHURJ_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
     gene            15939..18551
                     /gene="bcsA"
                     /locus_tag="ACHURJ_000420"
     CDS             15939..18551
                     /gene="bcsA"
                     /locus_tag="ACHURJ_000420"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HFR1279500.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPLDPVRYLIQTCWLLIGTSRKETPKPRRR
                     AFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSLIL
                     ALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRYTS
                     TLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVDIF
                     VPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIARTTH
                     EHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHFFS
                     PDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIAVE
                     TVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLDNP
                     LTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFVLP
                     HMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGGLV
                     EEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVLGG
                     AVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQILEG
                     QKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWALWQ
                     DSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFILRRPER
                     SETAQPSDQALAQQ"
     gene            18562..20901
                     /gene="bcsB"
                     /locus_tag="ACHURJ_000421"
     CDS             18562..20901
                     /gene="bcsB"
                     /locus_tag="ACHURJ_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFR1279501.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYMPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETRSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERVWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            20908..22014
                     /gene="bcsZ"
                     /locus_tag="ACHURJ_000422"
     CDS             20908..22014
                     /gene="bcsZ"
                     /locus_tag="ACHURJ_000422"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFR1279502.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            21996..25469
                     /gene="bcsC"
                     /locus_tag="ACHURJ_000423"
                     /pseudo
     CDS             21996..25469
                     /gene="bcsC"
                     /locus_tag="ACHURJ_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
     gene            25551..27539
                     /gene="hmsP"
                     /locus_tag="ACHURJ_000424"
     CDS             25551..27539
                     /gene="hmsP"
                     /locus_tag="ACHURJ_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFR1279503.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            27722..29008
                     /gene="dctA"
                     /locus_tag="ACHURJ_000425"
     CDS             27722..29008
                     /gene="dctA"
                     /locus_tag="ACHURJ_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFR1279504.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            29228..30724
                     /locus_tag="ACHURJ_000426"
     CDS             29228..30724
                     /locus_tag="ACHURJ_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFR1279505.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(30820..31749)
                     /gene="kdgK"
                     /locus_tag="ACHURJ_000427"
     CDS             complement(30820..31749)
                     /gene="kdgK"
                     /locus_tag="ACHURJ_000427"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFR1279506.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDISLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSTAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            31981..32748
                     /gene="pdeH"
                     /locus_tag="ACHURJ_000428"
     CDS             31981..32748
                     /gene="pdeH"
                     /locus_tag="ACHURJ_000428"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFR1279507.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLGDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNMAVLAL"
     gene            32818..34878
                     /locus_tag="ACHURJ_000429"
     CDS             32818..34878
                     /locus_tag="ACHURJ_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFR1279508.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(35112..36434)
                     /locus_tag="ACHURJ_000430"
     CDS             complement(35112..36434)
                     /locus_tag="ACHURJ_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1279509.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLLILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETQHQSL"
     gene            complement(36845..37858)
                     /gene="yhjD"
                     /locus_tag="ACHURJ_000431"
     CDS             complement(36845..37858)
                     /gene="yhjD"
                     /locus_tag="ACHURJ_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFR1279510.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(37907..38806)
                     /locus_tag="ACHURJ_000432"
     CDS             complement(37907..38806)
                     /locus_tag="ACHURJ_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1279511.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLNELDGLFQQDATSISGKLRIDIPPGI
                     AKNLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            39326..39928
                     /locus_tag="ACHURJ_000433"
     CDS             39326..39928
                     /locus_tag="ACHURJ_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFR1279512.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            complement(39979..41628)
                     /locus_tag="ACHURJ_000434"
     CDS             complement(39979..41628)
                     /locus_tag="ACHURJ_000434"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFR1279513.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVKRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            42032..43429
                     /gene="ccp"
                     /locus_tag="ACHURJ_000435"
     CDS             42032..43429
                     /gene="ccp"
                     /locus_tag="ACHURJ_000435"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFR1279514.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            43640..>43881
                     /locus_tag="ACHURJ_000436"
     CDS             43640..>43881
                     /locus_tag="ACHURJ_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFR1279515.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
BASE COUNT        10544 a        11003 c        11919 g        10415 t
ORIGIN      
        1 tcccgttagc actaccggaa aattacccgc tgccggggat tatagcggta ttgctaccat
       61 acatgtcgaa ctggagtaac agtcatgaag cgaacaatac ttgcgttatg cctgctgcct
      121 ctttcctttt cgtcattcgc ggcaaccacg gatttagggc cgaaaggaac attgaaattc
      181 acccttaaaa tcagccaggg tgcttgcgaa ctggtgaaag acagcgttga ggttgacatg
      241 ggcaccgcgg ttctcaaaaa gccggtaaga atcggaacgg aaattaatcc cactcctttt
      301 tccatcggcc tgaaaaactg tagcgaagtg gtcagagcct acgtgactat ggacggtaca
      361 cccaatgcag acgactcaaa tctttttgcg ctggatgcgg gtggcgctac aggcgttggg
      421 ctgaaaatca aaaccgctgc tggagtacaa cagttcccgg aaaatacaac gccgataccg
      481 gttgagtttg caattaacgg tgatggcgca catcaactga actatatcgc cagctacgtg
      541 cctattcgcg ccgatgcaac tattggtcgc gcagatgcaa cggtcgactt tactgtacag
      601 tacagtacga ataatttcac atcttaaggg ccagttaact ggcccttttt actgcttttt
      661 gtaacgctaa agtctctttt caaacttgca tttttgtaaa tttgtgcttc atgcacactc
      721 tttccccaca ctttttcgct ttgctgtggt ctacttattc gcgcgtgtag attttactta
      781 tctgactacc tccgcacttt tcccctgccg ggcctgaaaa gccgctaagc agggtgttat
      841 cacctgtttg tccagggttt gtttgcatga gatacatcaa atcgattaca cagcagaagc
      901 tgagcttttt gcttgcaatc tatattggcc tttttatgaa tggcgcggtt ttttatcgcc
      961 gcttcggcag ctatgcgcac gattttaccg tctggaaagg cgtttctgct gttgttgaac
     1021 tggccgccac cgtactggtg accttctttt tactacgtct tctatcgctg tttggccgcc
     1081 gcagctggcg tattctggcg tcgctggtgg tgctcttttc cgcaggtgcc agctattaca
     1141 tgaccttcct taatgtggtc attggttatg gcatcatcgc ttccgtcatg accaccgata
     1201 tcgacctgtc aaaagaagtt gttggtctga actttattct ctggttaatc gccgttagtg
     1261 cattgcctct tatccttatc tggaataacc gctgtcgcta caccttgctc cgacaactgc
     1321 gaaccccagg gcagcgtatt cgcagcctgg cggtcgtcgt actggcgggt attatggttt
     1381 gggcaccgat tcgtttgctg gatatccagc agaagaaagt ggagagggcg accggcgttg
     1441 atttgccgag ttatggcggt gtcgtagcga actcttatct gccatcaaac tggctttctg
     1501 cgttggggct gtatgcctgg gcgcgggtcg atgaatcttc cgataataat tcattgctta
     1561 atccggcgaa gaagtttaca taccaggctc cacaaaacgt tgatgatact tatgtcgtgt
     1621 ttatcatcgg tgaaaccacg cgttgggacc atatgggtat tttcggctat gagcgtaata
     1681 ccacgccgaa actggcccag gagaaaaatc tggcggcgtt ccgtggttac tcctgtgata
     1741 ccgcaaccaa actctcactg cgttgcatgt ttgtacgtca ggggggcgcg gaagataatc
     1801 cgcagcgcac attaaaagaa cagaacattt tcgcggttct gaagcagtta ggattcagtt
     1861 ctgacctcta cgctatgcag agcgaaatgt ggttctacag caacacgatg gcggacaaca
     1921 ttgcttatcg tgagcagatt ggtgcggagc cacgtaatcg tggcaagccg gtagatgata
     1981 tgttgctggt agacgaaatg cagcaatcgc tagggcgcaa cccggatggt aagcatctga
     2041 tcattctgca taccaaaggt tcgcatttta actacaccca gcgttatccg cgtagcttcg
     2101 cgcagtggaa gccggaatgt attggtgttg atagcggctg taccaaagcg cagatgatca
     2161 actcctatga caactcggtg acctatgtgg atcactttat ctccagcgtg attgatcagg
     2221 ttcgcgataa gaaagcgatt gtgttctacg cagctgacca cggtgagtca attaatgaac
     2281 gcgagcacct gcacggcacg ccgcgtgaat tggcaccgcc ggagcagttc cgcgtaccga
     2341 tgatggtctg gatgtcagat aaatatctgg aaaatccggc caatgcgcag gcatttgcgc
     2401 agctgaaaaa agaagccgac atgaaagtgc cacgccgtca cgtagagctg tacgatacca
     2461 tcatgggttg tcttggctat acttcaccgg atggtggaat taacgaaaac aacaactggt
     2521 gtcacatccc gcaggcaaaa gaggcagcgg ctaactaagc ggcctgctga ctttctcgcc
     2581 gatcaaaagg cattttgcta ttaagggatt gacgagggcg tatctgcgca gtaagatgcg
     2641 ccccgcattc ggtgattggc gcagcctggt agcgcacttc gttcgggacg aaggggtcgg
     2701 aggttcgaat cctctatcac cgaccaaatt cgaaaagcct gctcagcgag caggcttttt
     2761 tgcatttgaa gcccatgagg atgagaacct ccgggggctg ggaggttcga ctcgacgtag
     2821 gcctgataag acgcgccagc gtcgcatcag gcaacggcac cgcacttatt gtcggatgcg
     2881 gcgcgaccgc cttattcgac caacacacca cacattttcc tgccaacttt gcgccccata
     2941 tctcactatc cattaacgga tttgtgacag aaaccattgt ctttttttat atttgcttaa
     3001 atcttttttt gcgttactta tggctggcgc tggcattttc cgctgtgcgt tttagcgttc
     3061 atggtattaa tcgctcagat atccaccttt tcctcagatt tttttcatta aatgcataaa
     3121 acttaatcac caaatgttgc taaatattaa gctgattgtt ctggttatgt tggagtagca
     3181 caaatttctc tgctgcaaat agctgtttga agttttttaa tctttgtttg tgggttctca
     3241 ccgttggtgt aaatcccggt tggctgtatt gacgttttca cattctgttg acagattgta
     3301 ggtcacgagg ggcattttat ggaggatccg cactgttaca ctgatgttaa ttagtacggc
     3361 atccccacct cataacgttg acccgaccgg gcaaaaaaca aaaaaggtca ggtagcgaca
     3421 acccactgca aagggttaaa acaacaaaca tcacaattgg agcagaataa tgcgtatttc
     3481 cttgaaaaag tcagggatgc tgaagcttgg tctcagcctg gtggctatga ccgtcgcagc
     3541 aagtgttcag gctaaaactc tggtttattg ctcagaaggc tctccggaag ggtttaaccc
     3601 gcagctgttt acctccggca ccacctatga cgcctcttcc gtaccgcttt ataaccgcct
     3661 ggttgaattt aaaatcggca ccactgaagt gataccgggc ctcgctgaaa agtgggaagt
     3721 cagctaagac ggtaaaatct ataccttcca tctgcgtaaa ggtgtgaagt ggcacgacaa
     3781 taaagaattc aaaccgacgc gtgaactgaa cgccgatgac gtggtgttct cgttcgaccg
     3841 tcagaaaaac gcgcaaaacc cgtaccataa agtctctggc ggcagctacg aatacttcga
     3901 aggcatgggc ttgccggagc tgatcagtga agtgaaaaag gtggacgaca acacagttca
     3961 gttcgtactg actcgcccgg aagcgccgtt cctcgctgat ctggcaatgg acttcgcctc
     4021 tattctgtca aaagaatatg ctgatgcgat gatgaaagcc ggtacaccgg aaaaactgga
     4081 cctcaaccca atcggaaccg gtccgttcca gttacagcag taccaaaaag attcccgtat
     4141 ccgctacaaa gcgtttgatg gctactgggg caccaaaccg cagatcgata cgctggtctt
     4201 ctctattacc cctgatgctt ccgtgcgtta cgcgaaattg cagaagaatg aatgccaggt
     4261 gatgcagtac ccgaacccgg cagatatcgc tcgcatgaag caggataaat ccatcaacct
     4321 gatggaaatg ccggggctga acgtcggcta cctctcgtat aacgtgcaga aaaaaccgct
     4381 ggatgacgtg aaagttcgcc aggcactgac ctacgcggtg aacaaagacg cgatcatcaa
     4441 agcggtttat cagggcgcgg gtgtatcagc gaaaaacctg atcccgccaa ccatgtgggg
     4501 ctataacgac gacgttcagg attacaccta cgatcctgaa aaagcgaaag ccttgctgaa
     4561 agaagcgggt ctggaaaaag gtttctccat cgacctgtgg gcgatgccgg tacaacgtcc
     4621 gtataacccg aacgctcgct gcatggcgga gatgattcag gcagactggg cgaaagtggg
     4681 cgtgcaggcc aaaatcgtca cctacgaatg gggtgagtac ctcaagcgtg cgaaagatgg
     4741 cgaacatcag acggtaatga tgggctggac tggcgataac ggggacccgg ataacttctt
     4801 cgccaccctg ttcagctgcg ccgcctctga acaaggttct aactactcaa aatggtgcta
     4861 caaaccgttt gaagatctga ttcaaccggc gcgtgctacc gacgaccata acaaacgcgt
     4921 tgaactgtac aagcaatcgc aggtggtgat gcacgatcag gctccggcac tgatcatcgc
     4981 tcactccacc gtgtttgaac cggtacgcaa agaagtcaaa ggctatgtgg ttgatccatt
     5041 aggcaaacat cacttcgaaa atgtctctat cgaataatta aaagccatac aagactgatg
     5101 gtaaaggcaa aactgcctga tgtgctccgc ttatcaggcc tacgaaaatt ctgcaatgta
     5161 ttgaatttac acgattttgt aggccggata aggcgttaac gccgcatccg gcataaacaa
     5221 agcgcacttt gtcaacaatc tgtatacccg gtggcgctgt gcctctgcgt gcagcgccat
     5281 ccggcagcaa tacttcattc tctgtccgac aagggcggga attgatttgt gagcaataca
     5341 gacacgcagt tccaggctgc gggtcactat agagaatccg ggttatgttg cagtttattc
     5401 tccgacgttt gggactcgtc atccccacgt ttatcggtat tacccttctc acatttgcct
     5461 ttgtccacat gatcccgggc gatccggtga tgatcatggc aggcgaacgt gggatctccc
     5521 ctgagcgtca cgcgcagctg ctggctgaac tcggcttaga taaaccgatg tggcagcagt
     5581 atctccatta catttggggc gtaatgcacg gcgatctagg catttcaatg aaaagccgaa
     5641 ttccagtatg ggaagagttc gtgccgcgct ttcaggccac gctggaactt ggcgtctgcg
     5701 cgatgatttt tgctacggca gtcggtattc cggttggtgt gctggctgcg gttaaacgcg
     5761 gttccatttt cgatcacact gcagttggcc tggcgctgac cggctactcg atgccgatct
     5821 tctggtgggg catgatgctg atcatgctgg tttcggtgca ctggaacctg acgcccgcct
     5881 ccggtcgcgt gagcgatatg gtgttcctcg atgactccaa tccgttaacc ggttttatgc
     5941 taatcgacac cgccatctgg ggtgaagacg gcaactttat cgatgccgtc gcccatatga
     6001 tcttgcctgc cattgtgctg ggtactattc cgctggcggt cattgtgcgt atgacacgct
     6061 cctcgatgct ggaagtgctg ggcgaggatt acatccgcac cgcgcgcgcc aaagggctaa
     6121 cccgcatgcg ggtgattatc gtccatgcgc tgcgtaacgc gatgctgccg gtggtgaccg
     6181 ttatcggcct gcaggtggga acattgctgg cgggggcgat tctgaccgaa accatcttct
     6241 cgtggcccgg tctgggacgc tggttgattg acgcactgca acgccgcgac tatccggtag
     6301 tgcagggcgg cgtattgctg gtggcgacga tgattatcct cgtcaacttg ctggtcgatc
     6361 tgctgtacgg cgtggtgaac ccgcgtattc gtcataagaa gtaaggggac atcatgtcac
     6421 aggttactga aaataaagtg attagcgcac cggtgccgat gaccccgtta caggagttct
     6481 ggcactattt taaacgcaac aaaggcgcgg tcgtcgggct ggtttacgtc gtcatcgtgc
     6541 tgttcatcgc gatctttgcc aactggattg caccctataa cccggcggaa cagttccgcg
     6601 atgcactgct cgccccgcca gcctggcagg aaggcggcag catggcgcac ttgctgggca
     6661 ccgatgacgt aggccgtgat atgctgtcgc gcctgatgta cggtgcgcgc ctgtcgctgc
     6721 tggttggctg tctggtagtt gtgttatcgc tgattatggg cgttattctc ggcctgatcg
     6781 ccggttactt tggcggccta gtcgataaca tcattatgcg cgtggtcgat atcatgctgg
     6841 cgctgccaag tctgctgctg gcgctggtgc tggtggcaat tttcggcccg tcgattggta
     6901 acgccgcgct ggcactgacc ttcgttgcct tgccgcacta tgtgcgctta acccgcgccg
     6961 ccgtgctggt ggaagttaac cgcgattacg tcaccgcgtc tcgcgtggcg ggtgccgggg
     7021 cgatgcgtca gatgtttatt aacatcttcc cgaactgcct tgcgccgctg attgttcagg
     7081 cgtcgctcgg tttctctaac gccattctcg atatagctgc tcttggtttc ctcggcatgg
     7141 gggcacagcc gccaacgccg gagtggggca ccatgctctc cgacgtgttg cagttcgcgc
     7201 aaagcgcctg gtgggtcgtg accttcccgg gtctggcgat cctgctgacg gtgctggcat
     7261 ttaacctgat gggtgacggt ctgcgtgacg cgctcgatcc caaactgaag cagtaagagg
     7321 ttcgagatgg cgttattaaa tgtagataaa ttatcggtgc atttcggcga cgaaagcgca
     7381 ccgttccgcg ccgtagaccg catcagctac agcgtaaaac agggcgaagt ggttgggatt
     7441 gtgggtgagt ccggctccgg taagtcggtc agttccctgg cgattatggg gctgattgat
     7501 tatccgggcc gcgtaatggc ggaaaaactg gtgtttaacg gtcaggattt gcagcgtata
     7561 tcagaaaaag agcgccgcaa cctggtgggt gccgaagtgg cgatgatctt ccaggacccg
     7621 atgaccagcc ttaacccgtg ctacaccgtg ggtttccaga tcatggaagc gattaaggtg
     7681 catcagggcg gcaacaaaag tacccgccgt cagcgggcga ttgacctgct gaatcaggtc
     7741 ggtattcccg atccggcttc gcgtctggat gtttacccgc atcagctttc cggcggcatg
     7801 agccagcgcg tgatgatcgc catggcgatt gcctgtcgac caaaactgct gattgccgat
     7861 gagccgacca ccgcgctgga cgtgaccatt caggcgcaaa tcatcgaact actgctggag
     7921 ctacagcaga aagagaacat ggcgctggtg ttgattaccc atgacctggc gctggtggcg
     7981 gaagctgcgc ataaaatcat tgtgatgtat gcaggccagg tggtggaaac gggcgatgcg
     8041 cacgccatct tccatgcgcc gcgtcacccg tatactcagg cattgctgcg tgcgctgcca
     8101 gaatttgctc aggacaaaga acgtctggcg tcgttgccag gtgtcgttcc cggcaagtac
     8161 gaccgcccga acggctgcct gcttaacccg cgctgcccct atgccactga cagatgtcgc
     8221 gctgaagaac cggcgctgaa tatgctcgct gacgggcgtc agtccaaatg ccattaccca
     8281 cttgatgatg ccgggaggcc gacactatga gtacgcaaga ggccaccctg caacaaccgc
     8341 tgttgcaggc tatcgacctg aaaaaacatt atccggtgaa gaaaggcatg ttcgcgccgg
     8401 aacgtctggt taaagcgctg gatggcgttt cgtttaacct tgaacgtggc aaaacgctgg
     8461 cagtagtggg cgaatctggc tgcggtaaat cgaccctcgg tcggttgctg acgatgattg
     8521 aaacgcccac cggtggcgag ctgtattacc aggggcagga tctgcttaag cacgatccgc
     8581 aggcgcagaa gctgcgtcgg cagaaaatcc agatcgtctt ccagaaccct tacggttcgc
     8641 tgaatccgcg taaaaaagtc gggcaaattc ttgaagagcc gctgctgatc aacaccagct
     8701 taagcaaaga acagcgtcgg gaaaaagccc tgtcgatgat ggcgaaagtc ggcctgaaaa
     8761 ccgagcacta tgaccgctat ccgcatatgt tctccggcgg tcagcgtcag cgtatcgcca
     8821 tcgcccgtgg tctgatgctc gacccggatg tggtgattgc cgatgaaccg gtttccgcgc
     8881 tggatgtttc agtgcgcgcg caggtgctga atctgatgat ggatttgcag caggagttgg
     8941 ggctgtctta tgtctttatc tcccacgacc tgtcggtggt ggagcacatt gctgatgaag
     9001 tgatggtgat gtacctgggc cgctgcgtgg agaagggaac gaaagaccaa atcttcaata
     9061 acccgcgcca tccgtacact caggcgctac tttccgcgac gccgcgcctg aacccggacg
     9121 atcgccgcga gcgcatcaag ctcagcggtg aactaccaag cccactgaat ccaccgccgg
     9181 gttgcgcctt caacgcccgc tgtcgtcggc gcttcggccc ctgcacccag ttgcagccgc
     9241 agctaaaaga ctacggcggt caactggtag cttgttttgc tgttgatcag gatgaaaatc
     9301 cgcagcgtta actcacaaaa ccggggccat gcgctccggt ttactcaatc agcttcaaca
     9361 gtggcgacaa gcatagcaaa atgccgtaaa gcagaattag ccaggcttta aatccgcgaa
     9421 gtttttccag ttgtgcgact ttatagatca ggaaaaacgg aatgaggcac gacacaatgc
     9481 catataacgg gcttcccaac tgaaagaaca ccagcaccga tacacgaaac gaaacccaaa
     9541 tcgtcaacgt aatgacgata aaagcgcaga tcgccagagt cagcatgcgt gagttaattt
     9601 tcttggtatc aataattcgg cttaacagat tgagaataat gcctttaata gcctcgtgga
     9661 aaccgagata aatgccaaag aatgcggtca gtacggcaaa gatattcagc accgtagagg
     9721 tgatatgaat gatatgtcca gggatcacct gcgcggccag tgccagtgcc gagatatttt
     9781 gttcaaaggc agaaacggct tcttcgtggc taattgagaa ggtaaacgaa aaggcaaaaa
     9841 acaggatcac cgcgatgagc gtgatataac taattcggtg ggtacgcagc gcgagccggg
     9901 ttgccagtac tttatctgct tcccgtttac gataggcaat attcattggg ttaagtacct
     9961 gaataaatac cgcagaaaag aagcaaaatg gaattgtaag caggacatcg cggaaaaaga
    10021 cggaggcttg cgggaaggcg gttatattgg cgaaattcca gtgcgggatc atcgcaaaac
    10081 cgaacacgac aataatccct actttgacca ccaccattgg cccggaaatc ttaaacagta
    10141 atcgttcacc accagacgca atcgctacca gtacggcgaa aatagcgact ttataaagta
    10201 gagattgtga aagatcggca tcggttaaac cgaaggtttt caggtacgag gcgctgtcga
    10261 aaaccacgga gagagagtag ataaaaatcc cgtggataat catcaaaaag tagataaccc
    10321 cgaggaaaat tccccagttc ttccccaggt aatgactgat aatatcggtg tagtcattac
    10381 aggaatcgct ttcagaaagg gtttttaaat aaatgtcctg cactacccag gtggcaggat
    10441 aagcaatgat cgctgcggta ataaataccc aaattccctt caagccaatt tgtactggca
    10501 tcagcacggt tccggcacca atcgccatgc ctatgcataa taaaacccag ccgaagtcgt
    10561 agcgtgtaaa cggcaatttc tgattgtgtt tcgatagtgt gttgtgctgc ataaagtcct
    10621 gtccagaaag aagtcggcct cgcaaaaatg aataaaccca gtttctcttg cgcaacaggt
    10681 ggagaagtcg aatacgcagt atgtaaaaaa tgaggcaaca gattcactgg ggaggtcgcg
    10741 aggaggcgca attctacaaa attgcgcatt tcactaccag tctggagtca gcccggatat
    10801 gaactgcaac tattgcaaat aaagaaatta agctggaagg catcgcgcaa tgaggaagat
    10861 tgccgggtgc tgacagtaaa aagcaatcgt aaaatcagcg gcaagccgaa agggttccgg
    10921 tgaggcgcaa tgttgcgggg gctttatccc tggtggcatt ggttgctgga gagagaaaac
    10981 ccccgcacgt tgcaggtatg cacctgacaa catcacgggg gctaatcttg aacccaaaca
    11041 actcaagaat agccgcgaaa cgttggcatt acaacacagg cggctatatg acgctcgcag
    11101 aactgggcat ggccttctgg catgatttag cggctccggt cattgctggc attcttgcca
    11161 gtatgatcgt gaactggctg aataagcgga agtaacgtgt catgcgggcg tcaggctgcc
    11221 gtaatggcaa tttgcgcccg gatcaggccg cagaggttaa actctgcggc ctttttcgtg
    11281 cttactgcgg gtaaggcacc cagtcgccgc cgttcaggcg aacgtacggt ttatcctggt
    11341 attgaataac tactgcattt gagttctcgg agaccggtgc tgtttgtggc aacccactgg
    11401 tgagtttttt ccagtcaaca ttgtcttcgg tgaaaatctt gccatcaaga acgcgaacca
    11461 ccagatcgga gatagccagg aagctgctcg gttggtcgat gacaatcggt gccccctgat
    11521 gtggtgcctt catgccgaag aatttcaccc caacggggac gtctgtgata gacgggctag
    11581 ggatatcacg taggccagat acctgcattc tgtcgccctt cagcgcgccg ccgtgttccg
    11641 gcaccacgac caccatcact ttacgacccg atttctccag ttcagtaaag aaggcgtcca
    11701 gttcatcaaa gaatttctgc gcccgcgctt tgtaatccgc tgttttgctg acccccggat
    11761 aatggttgcc gtcatgcagt ggaagcgtgt tgtagaacgt ggcactacgg ctgtttttat
    11821 ctttttcggt aacgtccagc cagcggttaa gcacggtggt atcgtcataa accggcgaac
    11881 catcaaagcc cagcaaaata accggcagat ttgtttgatc catcaattca gtctgcatgc
    11941 cgccattttc gcgaacttct ttcaaaaaac cgccgaactg gccgttatgt cccatcatca
    12001 ggtgctgggt aaagcccagt ttcgaaaggt tatcaaacag atagcagtcg ttatttgccg
    12061 gttgatacag attagtgtgc gaagtctgcc cgcagctggc gcgcagtaaa cggatcgccg
    12121 ccgggccact gtaggaggtg gcggagttaa agttcttgaa ctcaatatcg aaatgcgacc
    12181 acagtggatg cgacatcaac ccggcggctt ctatatccga ccaggaaagc gaacagatgt
    12241 taatcaccag tagttcaaat ggctgagcat cagcgggcag cgaagacggg aaggtcgatt
    12301 tacgtttcgc ctccgcgtta tagaaattat tcagccaggc gttaaggttc gccgttgttg
    12361 gcggtgtagt ttgtgcgggc atatcaccca ctaccggtgc gccacccgtc gtctcaacgg
    12421 ttgccgctgc gttaccaccc gtcgttgtta cagtggtcgt cggttgtccg gctggccaca
    12481 aggagaaact tggtcccgcc agggtaagta cgttcagcca tagcagtatg gcaaccacaa
    12541 aaacggtaat gcgaatccat tgtgacagga ataaccaggc cactaataaa acaaaaatgg
    12601 ccccaatcat ctgccagtta ataaagcgtg tgacaaggtc gattaaataa tcggtactga
    12661 accccgccac ctgcgaaccc tggctcatta tgctttccgg gccaggcaac caggtgtcat
    12721 gccagaacaa agcaaagccg atcggcaggg caatccagtg gcgcaagcga tgcaggctgt
    12781 agcgcggaat gggcatcagc agaaacgcgg caaacaccaa attgaggagc ggatggaagt
    12841 taagatatcc cgcccacaac aggccgaact taaccagaaa atagaagttc cagccggaaa
    12901 ggccgcgcca gtattgccag agggaagaag gcatggcggt attttgcgta aattgagtca
    12961 ttttttggtt gccctggctt tttccgtgcg gcgtaatgtc ccggccggtt taacataacg
    13021 aggtttagca aagaacaaac gtaaggtgtc gcgaatgcgt cgcaaagagt gccgcgccag
    13081 atagcccagc gggaaaaata tcagtgcgca aacgacaata atttcaatga tatcgctgat
    13141 ggtcatcatg atgagcgctc cacagcatca tctaacagtc gcatgggttc tggtattcgt
    13201 cgccagtggc gaccatcgtg ctcggcattg atgaccggtt tagaactttg cgttaaaggc
    13261 agcggcatgc cccattgttc tggggcaagc aggcgcatct gcaccagctc ggcactgatt
    13321 tgatcatctt caaaccagac catacggttt gagaaaatgt cgccagtagg caatgggaaa
    13381 atatgattca acgcggtatc cagatcgtta atccgacaga atgagagaaa cagcaccagc
    13441 cgattaccgc caatggtcat gatatcgccg gtacggttag ggcgacacag cgtcagggct
    13501 tgttcaacgc ggatacccgg taccggacgt agggcaacca gaacgccttt accgtgggca
    13561 ggtaatagag ggttattcat catgttgttg acggcattac agaacacatc ccacttctgg
    13621 aaaccacgca gtttgagcgg ctgggtcatt gacagcaagg tagtgatatc ttccggcaca
    13681 tagcgactaa acttctgccc ttgcacgctt tcgatcatcg tcagacaacg ggagagtggc
    13741 gcattccacg gaataaccat atttgcaccg caggccaata acaaacgttc atcggtggcg
    13801 cgcaggctag cggtattttc ccgcacgagg attttcatcg cactaccgcg ctggcgacgc
    13861 agggtatgaa tgctgcgggc cagtggctcg atttgcgcat tttgctgtaa agaaaagacc
    13921 accgtcgccg cctgagcggt acgggcttca ttgaacagga cttcgttatt gttgaacagt
    13981 tgccagtgtt ccgatagcgg cggcgcacct tccagtacag caacattact cagaatgcgt
    14041 ttttcgtcgc tgcgtggttg gatctccgcc tcttcgcttt gaactaatgt ccagatacca
    14101 ttttgttgct gaacgctaag ctgctgacgg gcgctgaccc ctttttcgtt gcaccagaag
    14161 gcaatatcca gcaaatgttg gtcaccctga aaacgcaaac tggcaagacc aaaaagtgaa
    14221 cggtattcct caagcaacaa tgaaaattgt ttatcgttat tatttccggg attaattacc
    14281 aaaagcgaac aatgggttaa cctgctccat ttattcattt tatccaacca tgagcgaagc
    14341 cgctcggcag gaatgttttg ccatgcgtta tttgcgcaaa caagaataaa aaggtaatta
    14401 tggggatcaa tagaacactg caaatcacgg gtcaaatagt atagaccttt ttcatgattt
    14461 agcattgaaa ataattttat tttttccggc ccttgataat catctaattg aaagattttc
    14521 gccggatcgc tgtccatgct aatgaccgcg acgtgtgcgg tttcagcctg ggatgcaatt
    14581 gtttgattcg ccagactgat agcatcttca tggcgatcga cgttaaacca ccagacgccg
    14641 cctgctggca tatgtcgcag ctcatcccat aatgatgaga taccgataga gaatacaggg
    14701 cccacaatgt ccctcatgtc accattttgt ctgattatca gtttactagc gaaagctaag
    14761 aaataaacct accattgaaa ttaaaactta tcagatttag catgtgaatc aatttagaag
    14821 gtcacaagac gtgtgatttt agtcttttcg tttttaactt tttctgttgc gtaacggaaa
    14881 gtcaaaaagt gagcaaattc ccgtctcgcc gtaaacaata aaccggcgaa atgctcacgg
    14941 ttagaattgt ctcatgaacg gtacggttat ttcataggga tcaagcaaaa tgaataacaa
    15001 tgaaccagat actctgcctg atcccgcgat aggctatatc ttccagaatg atattgtggc
    15061 gttaaagcag gcattttcac tgcctgatat tgattatgcc gatatttccc aacgcgaaca
    15121 gttggccgcg gcattaaaac gctggccgtt gctggcagag tttgcgcaac aaaagtaggg
    15181 gattggtgaa tggccgtact gggattgcag ggggtgcggg gaggcgtggg gacaacaacc
    15241 atcaccgccg cattagccta gtcattacaa atgttgggag aaaatgtcct ggtggtcgat
    15301 gcctgcccgg acaacttgtt gcgcctgtca tttaatgttg attttaccca ccgtcagggc
    15361 tgggccagag cgatgctgga tggccaggac tggcgtgacg ctgggttgcg ctacacctcg
    15421 cagctcgatt tgctgccttt tggtcagtta tccattgaag aacaagaaaa tccacagcac
    15481 aggcaaaccc ggctgagcga tatttgctcc ggcttacagc aactaaaagc cagcgggcgt
    15541 taccagtgga ttttaatcga cttaccgcgt gatgcctcgc agataaccca ccagctgctg
    15601 agtttgtgcg atcactcgct ggcaatcgtc aatgtggatg ccaactgcca tatccgactg
    15661 catcagcaag cgctgccgga tggcgcacat attttgatta atgacttccg tattggcagt
    15721 caggttcagg acgatattta ccagctttgg ttgcaaagcc agcgccgatt actgccgatg
    15781 ctcattcatc gtgatgaagc gatagctgaa tgcctggcgg ctaagcaacc agtaggtgaa
    15841 tatcgcagtg atgcgctggc ggctgaagag atactgacgc tggcgaactg gtgcctgttg
    15901 aactactccg ggctgaaaac gccagtcggg agtgcatcat gagtatcctg acccggtggt
    15961 tgcttatccc gccggtcaac gcgcggctta tcgggcgtta tcgcgattat cgtcgtcacg
    16021 gtgcgtcggc tttcagcgcg acgctcggct gtttctggat gatcctggcc tggattttta
    16081 ttccgctgga gcacccgcgc tggcagcgta ttcgcgcaga acataaaaac ctgtatccgc
    16141 atatcaacgc ctcgcgtccg ctggacccgg tccgttatct cattcaaaca tgctggttac
    16201 tgatcggtac atcgcgcaaa gaaacgccga aaccgcgcag gcgggcattt tcaggtctgc
    16261 agaatattcg tggacgttac catcaatgga tgaacgagct gcctgagcgc gttagccata
    16321 aaacacagca tcttgatgag aaaaaagagc tcggtcattt gagtgccggg gcgcggcggt
    16381 tgatcctcgg tatcatcgtc accttctcgc tgattctggc gttaatctgc gttactcaac
    16441 cgtttaaccc gctggcgcag tttatcttcc tgatgctgct gtggggtgta gcgctgatcg
    16501 tacggcggat gccggggcgc ttctcggcgc taatgttgat tgtgctgtcg ctgaccgttt
    16561 cttgccgtta tatctggtgg cgttacacct ctacgctgaa ctgggacgat ccggtcagcc
    16621 tggtgtgcgg gcttattctg ctcttcgctg aaacgtacgc gtggattgtg ctggtgctcg
    16681 gctacttcca ggtcgtatgg ccgctgaatc gtcagccggt gccattgccg aaagatatgt
    16741 cgctgtggcc gtcggtggat atctttgtcc cgacttacaa cgaagatctc aacgtggtga
    16801 aaaataccat ttacgcctcg ctgggtatcg actggccgaa agacaaactg aacatctgga
    16861 tcctcgatga cggcggcagg gaagagtttc gccagtttgc gcaaaacgtg ggggtgaaat
    16921 atatcgcccg caccactcat gaacatgcga aagcgggcaa catcaacaat gcgctgaaat
    16981 atgccaaagg cgagttcgta tcgattttcg actgcgacca cgtaccaacg cgatcgttcc
    17041 tgcaaatgac catgggctgg ttcctgaaag agaaacagct ggcgatgatg cagacgccgc
    17101 accacttctt ctcgccggac ccgtttgaac gcaacctggg gcgttttcgt aaaacgccga
    17161 acgaaggcac gctgttctat ggtctggtgc aggatggcaa cgatatgtgg gacgccactt
    17221 tcttctgcgg ttcctgtgca gtaattcgtc gcaaaccatt ggatgaaatt ggcggtattg
    17281 ctgtcgaaac ggtaaccgaa gatgcgcaca cgtctctgcg cctgcaccgt cgtggctaca
    17341 cctctgcata tatgcgtatt ccgcaggcgg cggggctggc gaccgaaagt ctgtcggcgc
    17401 atatcggtca gcgtattcgc tgggcacgcg ggatggtgca aatcttccgt ctcgataacc
    17461 cgcttaccgg taaagggctg aaattcgccc agcggctgtg ctacgtcaac gccatgttcc
    17521 acttcttgtc gggcattcca cgactgatct tcctgactgc gccgctggcg ttcctgctgc
    17581 ttcatgccta catcatctat gcgccagcgt tgatgatcgc cctgttcgtg ctgccgcata
    17641 tgatccatgc cagcctgacc aactcgaaga tccagggcaa atatcgccac tctttctgga
    17701 gtgaaatcta cgaaacggtg ctggcgtggt atatcgcacc accgacgctg gtggcgctga
    17761 ttaacccgca caaaggcaaa tttaacgtca ccgccaaagg tggactggtg gaagaagagt
    17821 acgtcgactg ggtgatctcg cggccctaca tcttccttgt tctgctcaac ctggtgggtg
    17881 ttgcggtcgg catctggcgc tacttctatg gcccgccaac cgagatgctc accgtggtcg
    17941 tcagtatggt gtgggtgttc tacaacctga ttgttcttgg cggcgcagtt gcggtatcgg
    18001 tagaaagcaa acaggtacgc cgatcgcacc gcgtggagat gacgatgccc gcggcaattg
    18061 cccgcgaaga tggtcacctc ttttcgtgta ccgttcagga tttctccgac ggtggtttgg
    18121 ggatcaagat caacggtcag gcgcagattc tggaagggca gaaagtgaat ctgttgctta
    18181 aacgcggtca gcaggaatac gtcttcccga cccaggtggc gcgcgtgatg ggtaatgaag
    18241 ttgggctgaa attaatgccg ctcaccaccc agcaacatat cgattttgtg cagtgtacgt
    18301 ttgcccgtgc ggatacatgg gcgctctggc aggacagcta cccggaagat aagccgctgg
    18361 aaagtctgct ggatattctg aagctcggct tccgtggcta ccgccatctg gcggagtttg
    18421 cgccttcttc ggtgaagggc atattccgtg tgctgacttc tctggtttcc tgggttgtat
    18481 cgtttattct gcgccgcccg gagcggagcg aaacggcaca accatcggat caggctttgg
    18541 ctcaacaatg atgataacgc gatgaaaaga aaactattct ggatttgtgc agtggctatg
    18601 gggatgagtg cgttcccctc tttcatgacg caggcgacgc cagcaacgca accactgatc
    18661 aatgctgagc cagctgtagc cgcccagacg gaacaaaatc cgcaggtggg gcaagtgatg
    18721 ccgggcgtgc agggcgctga tgcgccagtc gtggcgcaga acggtccttc gcgtgatgtg
    18781 aagctgacct ttgcgcaaat tgcgcctccg ccgggcagca tggtgctacg tggcattaac
    18841 ccgaacggca gcattgagtt tggtatgcgc agcgatgaag tggtgacgaa ggcgatgctc
    18901 aacctcgaat acatgccatc gccatcgtta ctgcctgtcc agtcgcagtt aaaggtttat
    18961 ctcaatgatg agctgatggg cgtactgcca gtgaccaaag aacagttggg taaaaaaacg
    19021 ctggcgcaaa tgcccattaa cccactgttt attaccgact tcaaccgtgt gcggctggag
    19081 tttgtcggcc attatcagga cgtgtgcgaa aacccggcca gcaccacgct ttggctggat
    19141 gttgggcgga gcagtggact ggatctgacc tatcagaccc tgaatgtgaa gaatgacctg
    19201 tcacacttcc cggtgccatt ctttgacccg cgcgataacc gtactaacac cttgccgatg
    19261 gtctttgcgg gtgcgccgga tgttgggctg caacaagcat ctgccattgt cgcctcgtgg
    19321 tttggttcgc gttctggctg gcgtgggcag aacttcccgg tgctctataa ccaactgccg
    19381 gatcgtaatg ccattgtctt tgcaaccaac gacaaacggc cggacttcct gcgcgatcat
    19441 ccggcggtaa aagccccggt gattgagatg atcaaccatc cgcagaatcc ttacgtcaaa
    19501 ctgctggtgg tgtttggtcg tgacgacaaa gacctgttgc aggcagcgaa aggtatcgct
    19561 cagggtaaca ttctgttccg tggtgaaagc gtggtagtga atgaagtgaa accgctgcta
    19621 ccgcgtaagc cgtacgatgc gccgaactgg gtacgtaccg atcgtccggt cacatttggc
    19681 gaactgaaaa cctatgaaga acagttacaa tccagcggtc ttgagccagc agcgattaac
    19741 gtttcgctaa acctgccgcc ggatctctac ctgatgcgca gtaccggcat tgatatggat
    19801 attaattacc gctacaccat gccgccggtg aaagacagtt cgcggatgga tatcagcctg
    19861 aataaccagt tcctgcaatc cttcaacctg agcagcaaac aggaggcgaa ccgcctgctg
    19921 ctgcggattc cggtattaca aggtttgctg gatggcaaaa cagatgtctc tattccggcg
    19981 ctgaaactgg gcgcgaccaa ccagctgcgc ttcgactttg agtatatgaa cccgatgccg
    20041 ggcggttcgg tggataactg tattaccttc cagccggtgc agaatcatgt ggtgattggt
    20101 gacgactcca ccatcgactt ctcgaagtat taccacttca tcccgatgcc ggatctacgc
    20161 gcctttgcta acgcgggctt cccattcagc cggatggcgg atctgtcgca aaccatcacc
    20221 gtgatgccga aagcgcctaa cgaagcacag atggaaacgt tgctgaatac tgttggtttt
    20281 atcggcgcac agacgggctt cccggcgatt aatctgacgg tgaccgatga tggcagcacc
    20341 attcagggca aagatgccga catcatgatc atcggtggta tcccggacaa actgaaagac
    20401 gataagcaga tcgacctatt ggtgcaggcg accgaaagct gggtgaaaac accgatgcgc
    20461 cagaccccgt tccccggcat tgtgccggac gagagcgatc gcgcggcaga aacccggtca
    20521 acgctgacct cttccggtgc gatggcggcg gtgattggct tccagtcgcc gtataacgac
    20581 cagcgcagcg tgattgcgct gttggcagat agcccacgcg gttatgaaat gcttaacgat
    20641 gcggtgaacg atagcggcaa acgcgccacc atgttcggtt cggtcgcggt gatccgcgag
    20701 tccggtatca acagcctacg tgttggcgac gtttattacg taggtcatct gccgtggttc
    20761 gagcgcgtgt ggtatgcgct ggcaaaccat ccgattctgc tggcggtgct ggcggctatc
    20821 agtgtgatat tgctggcatg ggtactgtgg cgtctgctgc gaattattag tcgtcgtcgt
    20881 cttaacccgg ataacgagta attgaagatg aatgtgttgc gtagtggaat cgtgacgatg
    20941 ctgttgttgg ctgcctttag tgttcaggca gcctgtacct ggcctgcctg ggagcagttt
    21001 aaaaaggatt acatcagtca ggaagggcgc gtcatcgacc ccagcgacgc gcgcaaaatc
    21061 accacctccg aagggcaaag ttacggtatg ttctttgccc tggcggctaa cgaccgtgca
    21121 gctttcgata atattctcga ctggacgcag aacaatctcg ctcagggttc tttaaaagaa
    21181 cgtttgcccg cctggctgtg gggcaagaaa gagaacagta agtgggaagt gctggacagc
    21241 aattcggcct ccgatggtga tgtctggatg gcctggtcgt tgctggaggc ggggcgtttg
    21301 tggaaagagc agcgttatac cgacatcggc agcgcgttgc taaaacgtat cgcgcgggag
    21361 gaagtggtga cggtgcctgg gctgggttcc atgttgttac cgggcaaagt gggttttgct
    21421 gaggataaca gctggcgttt taaccccagc tacctgccgc cgacgctggc gcagtatttc
    21481 acccgctttg gcgcgccgtg gactacgctg cgcgaaacca atcaacgttt attgctggaa
    21541 accgccccga aaggcttttc gccagactgg gtgcgttatg agaaagacaa aggctggcag
    21601 ctaaaagccg aaaaaacatt gatcagcagc tacgacgcca tccgcgttta catgtgggta
    21661 ggcatgatgc ctgacagcga tccgcagaaa gcgcggatgc tcaaccggtt taaaccgatg
    21721 gcgacattca ctgagaaaaa cggttatccg ccggaaaaag tggatgtggc tacggggaaa
    21781 gcgcagggta aaggaccagt tggtttttct gccgccatgc tgcccttttt acaaaaccgc
    21841 gatgcgcagg ccgttcagcg tcagcgcgtg gccgataact ttcccggcag cgatgcctat
    21901 tacaactatg tgctgaccct gtttggacaa ggctgggatc aacaccgttt ccgcttctcg
    21961 acaaaaggtg agttattacc tgactggggc caggaatgcg caaattcaca ctaaacatat
    22021 tcacgctttc cctcggtctg gccgtcatgc cgatggtcga ggcagcacca accgctcagc
    22081 aacagttgct ggagcaagtt cggttaggcg aagcgaccca tcgtgaagat ctggtgcaac
    22141 agtcgttata tcggctggaa cttattgatc cgaataaccc ggacgtcgtt gccgcccgtt
    22201 tccgttcttt gttacgttag ggcgatattg atggcgcgca aaaacagctc gatcggctgt
    22261 cgcagttagc gccgagttca aatgcgtata aatcgtcgcg gactacgatg ctactttcca
    22321 cgccggatgg tcgtcaggca ctgcaacagg cacgattgca ggcgacgacc ggtcatgcag
    22381 aagaagctgt ggcgagttac aacaaactgt tcaacggtgc gccgccggaa ggtgacattg
    22441 ctgtcgagta ctggagtacg gtggcgaaaa ttccggctcg ccgtggcgaa gcgattaatc
    22501 agttaaaacg catcaatgcg gatgcaccgg gcaatacggg cctgcaaaac aatctggcgc
    22561 tattgctgtt tagtagcgat cgccgtgacg aaggttttgc cgtcctggaa cagatggcaa
    22621 aatcgaacgc cgggcgcgaa ggggcctcta aaatctggta cgggcagatt aaagacatgc
    22681 ccgtcagtga tgccagtgtg tcggcgctga aaaaatatct ctcgatcttt agtgatggcg
    22741 atagcgtggc ggctgcgcaa tcgcaactgg cagaacagca aaaacagctg gccgatcctg
    22801 ctttccgcgc tcgtgcgcaa ggtttagcgg cggtggactc tggtatggcg ggtaaagcca
    22861 ttcccgaact acaacaggcg gtgcgggcga acccgaaaga cagtgaagct ctgggggcgc
    22921 tgggccaggc gtattctcag aaaggcgatc gcgccaatgc agtggcgaat ctggaaaaag
    22981 ccctcgcact ggacccgcac agcagcaaca acgacaaatg gaacagtctg ctgaaagtaa
    23041 accgctactg gctggcgatc cagcagggcg atgctgcgct gaaagccaat aatcctgacc
    23101 gggcagaacg cctgttccag caggcgcgta atgtcgataa caccgacagt tatgcagtgc
    23161 tggggctggg cgatgtggcg atggcgcgaa aagattatcc cgccgccgaa cgttattatc
    23221 agcagacctt gcgtatggac agcggcaaca ctaacgccgt gcgcgggctg gcaaatattt
    23281 accgccagca atcgccagaa aaagctgaag cgtttatcgc ctcgctctct gccagtcagc
    23341 ggcgtagcat tgatgatatc gaacgcagcc tgcaaaacga ccgtctggca cagcaggcag
    23401 aggcactgga aaaccagggc aaatgggcgc cggcggcagc acttcagcgg caacgactgg
    23461 cgctggaccc cggcagcgta tggattactt accgactttc gcaggatctc tggcaggccg
    23521 gacaacgcag ccaggccgat acgttaatgc gcaatctggc gcagcagaag tcgaacgacc
    23581 cggagcaggt ttacgcttac gggctgtacc tctctggtca tgaccaggac agagcggcgc
    23641 tggcgcatat caacagcctg ccgcgcgcgc agtggaacag caatattcag gagctggtta
    23701 atcgactgca aagcgatcag gtgctggaaa ccgctaatcg cctgcgagaa agcggcaaag
    23761 aagctgaagc ggaagcgatg ctgcgtcagc aaccaccttc cacgcgtatt gacctcacgc
    23821 tggctgactg ggcgcagcag cgacgtgatt acaccgccgc ccgcgctgca tatcagaatg
    23881 tcctgacgct ggagccaact aacgccgatg ccattctggg gctgacggaa gtggatattg
    23941 ctgccggtga cacagcggcg gcacgtagcc agctggcgaa actgcccgct accgataacg
    24001 cctcgctgaa cacacagcgg cgcgtggcgc tggcgcaggc gcagcttgga gataccgcag
    24061 cggcgcagca gacgtttaat aagttgatcc cgcaggcaaa atctcagcca ccgtcgatgg
    24121 aaagcgcgat ggtgctgcgc gacggtgcga agtttgaagc gcaggcgggc gatccaaagc
    24181 aggcgctgga aacctacaaa gacgccatgg tcgcatccgg tgtgactacg acgcgtccgc
    24241 aggataacga cacattcacc cgactgaccc gtaacgacga gaaagatgac tggctgaaac
    24301 gcggcgtgcg cagcgatgcg gcgggcctct atcgccagca ggatcttaac gtcaccctcg
    24361 agcacgatta ctggggttcg agcggcaccg gtggttactc cgatctgaaa gcgcacacta
    24421 ccatgttgca ggtggatgcg ccgtattctg acgggcggat gttctttcgc agtgatttcg
    24481 tcaatatgaa cgtcggcagt ttctccacta atgccgatgg caaatgggat gacaactggg
    24541 gcacctgtac attacaggac tgtagtggca accgcagcca gtcggactcc ggtgccagcg
    24601 tggaggtcgg ctggcgaaat gacgtctgga gctgggatat cggtaccacg ccgatgggct
    24661 tcaacgtggt ggatgtggtc ggcggcatca gttacagcga tgatatcggg ccgctgggtt
    24721 acaccgttaa cgcccaccgt cggcccatct ccagttcttt gctggccttt ggtgggcaaa
    24781 aagactcccc gagcaatacc gggaaaaaat ggggtggcgt acgtgccgac ggtgtggggc
    24841 taagtctgag ctacgataaa ggtgaagcaa acggcgtctg ggcatcgctt agtggcgacc
    24901 agttaaccgg taaaaatgtc gaagataact ggcgcgtgcg ctggatgacg ggctattact
    24961 ataaggtcat taaccagaac aatcgccgcg tcacaatcgg cctgaacaac atgatctggc
    25021 attacgacaa agatctgagt ggctactcac tcggtcaggg cggttactac agtccgcagg
    25081 aatacctgtc gtttgctata ccggtgatgt ggcgggagcg cacggaaaac tggtcgtggg
    25141 agctgggggc gtctggctcg tggtcgcatt cacgcaccaa aaccatgccg cgttatccgc
    25201 tgatgaatct gatcccgacc gactggcagg aagaagctgc gcggcaatcc aacgatggcg
    25261 gcagcagtca gggcttcggc tacacggcgc gggcattact tgaacgacgt gttacttcca
    25321 actggtttgt tggcacggca attgatatcc agcaggcgaa agattacgca cccagccatt
    25381 tcctgctcta cgtacgttat tccgccgccg gatggcaggg tgacatggat ttaccgccgc
    25441 agccgctgat accttacgcc gactggtaag ttttcagata gcgcctctct taatgccgct
    25501 gcgatcgggt atactcgggc ggcaatctgg gatttccggg gggagacaat ttgcgcgtaa
    25561 gtcgctcgtt aacaatcaag cagatggcaa tggtggcagc cgttgtcctg gtgttcgttt
    25621 ttattttttg caccgttttg ctgttccatc tggtccagca gaatcgctat aacacggcta
    25681 cgcaactgga aagcattgct cgctctgtac gcgaaccctt atcttcagct attttgaaag
    25741 gcgatattcc cgaagcggaa gctattcttg ccagcattaa accggcaggc gtggtcagcc
    25801 gtgccgatgt agtgctgcct aaccagttcc aggcgctgcg taaaagtttt attccagagc
    25861 gcccggtgcc ggtaatggtt actcgcctgt ttgagctacc ggttcaaatc tcgctgggcg
    25921 tttactcgct cgaacgtccg gcaaacccgc agccaattgc ctatctggta ctacaggcgg
    25981 attccttccg tatgtataag ttcgtgatga gcaccctctc aacgttagtg accatttact
    26041 tacttttgtc gcttatcctg accgtcgcca tcagctggtg cattaaccgc ctgattttgc
    26101 atccgttacg caatattgct cgcgaactta acgccatccc agcccaggag cttgttggtc
    26161 accaactggc attaccgcgt ctgcatcagg acgatgaaat cggtatgttg gtgcgcagtt
    26221 acaacctcaa ccagcaattg ctgcagcgcc attatgaaga acagaacgaa aatgcgatgc
    26281 gcttcccggt gtcggatttg ccgaacaaag ccttgctgat ggagatgctg gagcaggttg
    26341 tcgcgcgtaa acaaaccacc gcgctgatga tcatcacctg tgaaaccctg cgtgatactg
    26401 cgggcgtgct gaaagaggcg caacgagaaa ttctgctgct gacgctggtg gaaaaactca
    26461 aatcggtact gtcgccacgt atgatcctcg cgcagattag cggttatgac tttgctgtca
    26521 ttgccaacgg tgtacaggaa ccgtggcacg caatcacctt aggtcagcaa gtgctcacta
    26581 tcatgagcga gcgcctgccg attgaacgta ttcaactccg tccgcactgt agcattggcg
    26641 tggcgatgtt ctacggcgat ctcaccgccg aacagcttta cagtcgcgct atttctgcgg
    26701 catttaccgc tcgccataaa ggcaagaatc agattcagtt ctttgatccg cagcagatgg
    26761 aagccgccca gaagcggttg acggaagaga gcgatatcct taatgcactg gaaaatcatc
    26821 agtttgctat ttggttacag ccacaggtcg agatgaccag cggtaaactg gtcagtgcgg
    26881 aagtgttact gcgtatccag caaccggatg gcagttggga cctgccggat ggcttaatcg
    26941 atcgcattga gtgctgtggg ctgatggtta ccgtcggtca ctgggtgctg gaagagtcct
    27001 gtcgattgct tgcagcctgg caagagcgcg gcattatgct gcccttgtcg gtaaacctct
    27061 ctgcgctgca actgatgcac ccaaatatgg tggcggatat gctggaactg ttaacccgct
    27121 atcgcattca accgggaaca ctgattctgg aagtgacaga aagccgacgt attgacgacc
    27181 ctcatgctgc ggtggcaatc ctccgtccgc tgcgcaatgc cggagttcgg gtggcgctgg
    27241 atgatttcgg catgggctac gcagggctgc gtcagctgca gcatatgaaa tcgttgccaa
    27301 tcgacgtact gaaaatcgac aaaatgtttg ttgaaggctt gccggaagat agcagcatga
    27361 ttgctgcaat tatcatgctg gcgcagagcc tgaacttaca aatgattgcc gaaggcgtgg
    27421 agactgaagc acaacgcgac tggctggcaa aagcgggcgt tggtattgcc cagggcttcc
    27481 tttttgctcg cccactccct attgaaatct tcgaagagag ttacctggaa gaaaagtagc
    27541 taccccaaac tgattacaaa actttaaaaa gtgctggttt gtgcgagcca gctcaaactt
    27601 tttaaccttt ttgtttcaat tatgatccag gtacatttct gtgatgttgt ctgggtgtta
    27661 ttttaaggcc gcaggtacct cataacctta caagacctgt agttttacta aaggacaccc
    27721 tatgaaaacc tctctgttta aaagccttta ctttcaggtc ctgacagcga tagccattgg
    27781 tattctcctt ggccatttct atcctgaaat aggcgagcaa atgaaaccgc ttggcgacgg
    27841 cttcgttaag ctcattaaga tgatcatcgc tcctgtcatc ttttgtaccg tcgtaacggg
    27901 cattgcgggc atggaaagca tgaaggcggt cggtcgtacc ggcgcagtcg cactgcttta
    27961 ctttgaaatt gtcagtacca tcgcgctgat tattggtctt atcatcgtta acgtcgtgca
    28021 gcctggtgcc ggaatgaacg tcgatccggc aacgcttgat gcgaaagcgg tagcggttta
    28081 cgccgatcag gcgaaagacc agggcattgt cgccttcatt atggatgtca tcccggcgag
    28141 cgtcattggc gcatttgcca gcggtaacat tctgcaggtg ctgctgtttg ccgtactgtt
    28201 tggttttgcg ctccaccgtc tgggcagcaa aggccaactg atttttaacg tgatcgaaag
    28261 tttctcgcag gtcatcttcg gcatcatcaa tatgatcatg cgtctggcac ctattggtgc
    28321 gttcggggca atggcgttta ccatcggtaa atacggcgtc ggcacactgg tgcaactggg
    28381 gcagctgatt atctgtttct atattacctg tatcctgttt gtggtgctgg tattgggttc
    28441 aatcgctaaa gcgactggtt tcagtatctt caaatttatc cgctacatcc gtgaagaact
    28501 gctgattgta ctggggactt catcttccga gtcggcgctg ccgcgtatgc tcgacaagat
    28561 ggagaaactc ggctgccgca aatcggtggt ggggctggtc atcccgacag gctactcgtt
    28621 taaccttgat ggcacatcga tatacctgac aatggcggcg gtgtttatcg cccaggccac
    28681 taacagtcag atggatatcg tccaccaaat cacgctgtta atcgtgttgc tgctttcttc
    28741 taaaggggcg gcaggggtaa cgggtagtgg ctttatcgtg ctggcggcga cgctctctgc
    28801 ggtgggccat ttgccggtag cgggtctggc gctgatcctc ggtatcgacc gctttatgtc
    28861 agaagctcgt gcgctgacta acctggtcgg taacggcgta gcgaccattg tcgttgctaa
    28921 gtgggtgaaa gaactggacc acaaaaaact ggacgatgtg ctgaataatc gtgcgccgga
    28981 tggcaaaacg cacgaattat cctcttaatc tcaccactta tgcccgcact cccttccccg
    29041 agtgcgggtg tttgcgcata attgacccct gtgcctgaac ttaccacctg ggtgagttca
    29101 ggtttatttc acttcttccg tgacattttc atgttcttgc ggtctaacac gaagtgtttt
    29161 taacgtcatt tagactgact gacaacgggc agttttttat taccaggata gttgatcagg
    29221 ggttcacatg cagggcacaa aaattcgact tttagcgggc ggtttgctga tgatggccac
    29281 tgctggctat gtgcaggcag atgcgctcca gcctgatcca gcatggcaac aggggacgct
    29341 ttccaacggt ttacagtggc aagtgctgac caccccccag cgtcccagcg atcgtgttga
    29401 aattcgcctg ctggttaata ccggttcgct cgccgaaagt acacaacaga gcggttacag
    29461 tcacgccatc cctcgtattg cgctaacgca aagcggtggc cttgacgcag cacaggcgcg
    29521 ttcattgtgg cagcagggga tcgaccctaa acgcccgatg ccgccggtaa ttgtctctta
    29581 tgacaccacg ctgtttaatc tgagtttgcc caataaccgt aacgatttgc tgaaagaagc
    29641 gctctcttat ctggcaaatg ccactggcaa attgaccatc acaccagaaa ccatcaacca
    29701 cgcgctgcaa agtcaggaca tggtggcaac ctggcctgcc gatactaaag agggctggtg
    29761 gcgctatcgt ctgaaagggt caaccttgtt aggtcacgat cctgccgatc cgctgaaaca
    29821 acccgttgaa gcggaaaaaa ttaaagattt ctatcagaaa tggtacaccc cggatgcaat
    29881 gacgctactg gtggtgggaa acgtggatgc gcgctcggtt gtcgaccaaa tcaacaaaac
    29941 gtttggcgaa ctgaaaggca aacgtgaaac gccagctccg gtgccgacgc tttctccgct
    30001 gcgtgcggaa gcggtgagta ttatgactga cgcggtgcgt caggaccggt tatctatcat
    30061 gtgggatacg ccgtggcagc cgattcgtga atcagccgca ctgctgcgct actggcgtgc
    30121 ggacctggcc cgcgaggcgc tgttctggca tgttcagcaa gcgttaagcg ccagtaacag
    30181 caaagacatc ggtcttggat ttgactgccg tgtgctgtat ctgcgtgcgc agtgtgccat
    30241 caacatcgaa tcaccaaacg acaagctgaa cagcaacctt aatctggtgg cgcgtgaact
    30301 ggcgaaggtt cgcgataaag gtctgccgga agaagagttc aatgcgttag tggcgcaaaa
    30361 gaaactggag ctgcagaaac tgtttgccgc ctatgcgcgg gctgataccg atattctgat
    30421 gggtcagcgg atgcgttcgt tgcaaaatca ggtcgtcgat atcgcgccgg agcagtatca
    30481 gaaactgcgg caggatttcc ttaacagcct gacggtggag atgttaaatc aggatctgcg
    30541 tcagcagttg tcgaatgata tggcgttaat actgctgcag ccgaaaggcg agccggaatt
    30601 taacatgaaa gcgttgcagg cggcctggga tcaaatcatg gccccatcga ctgctgctgc
    30661 cgccacctct gtcgccacgg atgacgtaca tcctgaagtg acggatattc cacctgcaca
    30721 gtaatgcctg atggtgatga gttagcacaa ccatcaggta tttatggatg agctggatag
    30781 aggggtaaca ctttatccag ccttttgcat atgctgcgtt tacgctggca tcgcctcacg
    30841 cgggataatc gcgccgcgat actgaataac ggtacttgcg gtcaggtgcc cgcgtttcgc
    30901 cgcgttttcc gcactgccgc ccgtcaaacg taccgccaga taaccggcgc tgaaagagtc
    30961 gccagctgcg gtggtatcga tcactttttc tttcggcagt ttcaccgccg gaacatccac
    31021 taacccttcg ccagcaatgg acaccaggca agaatccgcc ccgcgtttca ccaccacttc
    31081 tttcacgccc gcgttatggg tgcgcgcaat gacgtcttcc accggctgtt gaccccacag
    31141 cgcgtcttca tcgtccagcg tcaggaaggc gatatccgtg cattccagca tttgttggta
    31201 cacctgctgt gtctcttctt tgctggccca caggcgcgga cgatagttat tgtcgaaaat
    31261 cacttttccg ccgttggcgc ggcattcgcg cagcagggaa agcagctttt cgcggctggt
    31321 cgggcttaag atcgccaggc taatcccgct caggtagaga taatcgaaat tcgccagctc
    31381 ttcgcaaatc gccgtagact gctcactctc cagccagaat ttggcggcgg cttcgttccg
    31441 ccagtagtag aacgtacgct cgccggtgct gtcggtttca atgtagtaaa ggcccggcag
    31501 acggttttcc atccgttggg tcagggaaat atcaacgttc tcgccgtgcc aggcgtccag
    31561 catctgctgg ctaaaactgt ccgttcccag cgccgttacg taatgaacgg ttaatgccgc
    31621 aggatcgacc tgacgggcga tatagacgga agtgttcagg gtatcgccgc cgaaaccgcg
    31681 cttaacgtcc gcgcctttct cggaaagctc aatcatgcat tcgccaatca cggcaatctt
    31741 tttggacata gtcgtgaacc tgatctgtaa aaaattagcg ttagtttgcg ctgtggtggt
    31801 ttgctggtca acaatattaa aacagtgttc cattattttt ttgagccaga acgtattcct
    31861 gaaagattcc gttgtggagt gaggaaattt accccggcca ggcggtaaag ttctgccctt
    31921 acgcgccgat aatctttgtc gagtccgggc agcatcactt ttaaacacag gacatctttg
    31981 atgataaggc aggttatcca gcgaataagc aaccctgaag caagcatcga gagcttgcag
    32041 gaacggcgtt tttggttgca gtgtgagcgt gcttacacct ggcagccgat ctatcaaaca
    32101 tgcgggcggt taatggccgt ggagctatta acggtggtca cgcatccctt gaacccttcg
    32161 caacgcctgc cgccggatcg ctattttact gaaatcaccg tcagccatcg gatggaggtt
    32221 gtgaaagagc agattgattt gctggcgcaa aaagccgact tctttataga gcacggcctg
    32281 ctggcatcgg tcaatattga tggccctacg ctcatcgccc tgcgtcagca accaaaaatc
    32341 ctgcgccaaa ttgagcgtct tccctggctg cgtttcgaac tggtggagca tatccgtctg
    32401 ccgaaagatt caacctttgc ctcgatgtgt gaatttggcc cgctgtggct gggtgatttt
    32461 ggtaccggga tggcaaattt ctctgcacta agtgaagtgc gttatgacta catcaaaatc
    32521 gcgcgagaac tgtttgtgat gctgcgtcag tcgccggaag gacgcacact cttttctcag
    32581 cttttacatc taatgaatcg ctattgtcgc ggggtgattg ttgagggcgt agaaacgccg
    32641 gaagagtggc gtgatgtaca gaactcgccc gcattcgccg cacaaggctg gtttctttca
    32701 cgcccggcac cgatagaaac gctgaatatg gcggttctgg cgctataagc tgcctcattt
    32761 tccgcctggc tggactatct ttaggactgg cacaggaaga atatgaggaa gcgaacgatg
    32821 agcaaggcag gcaaaataac tgctgcgatt tcaggggctt tcttgttgtt gattgtcgtg
    32881 gcgatcattt tgattgcaac ttttgactgg aatcgactca aaccgaccat caaccagaaa
    32941 gtctctgcgg agttgaatcg tccgttcgct atccgtggcg atctgggcgt ggtgtgggag
    33001 cggcaaaaac aagaaactgg ctggcgcagc tgggtgccgt ggcctcatgt acatgcagaa
    33061 gacatcattc ttggcaatcc accggatatt cccgaagtca cgatggtgca tttgccgcga
    33121 gtagaggcaa cgctggcacc gctggcgctg ctgaccaaaa cggtctggct gccgtggatc
    33181 aagctcgaaa agcccgacgc gcgcctgatt cgcctctccg aaaagaacaa taactggacg
    33241 tttaatctcg ccaacgatga taacaaagac gcgaatgcaa agccgtcggc atggtcgttt
    33301 cggctggata atattctttt cgatcaaggg cggatcgcca ttgatgacaa agtaagcaaa
    33361 gcggatctgg aaatttttgt tgatccgtta ggcaagccgc tgccgttcag cgaagttact
    33421 ggatcgaaag gtaaagcgga taaagaaaag gtgggcgatt acgtttttgg cctgaaggcg
    33481 cagggacgtt ataacggtga accgctcacg ggtacgggaa aaataggcgg tatgctggcg
    33541 ctgcgtggcg aagggacgcc gtttccggta caggctgatt tccgttcagg taatacccgt
    33601 gttgcttttg atggcgtcgt gaatgaccca atgaagatgg gcggtgtcga tttacggctt
    33661 aaattttctg gcgattcact gggtgatctc tatgaactga cgggcgttct gctgcccgat
    33721 accccgccgt ttgaaacgga tggtcggctg gtagcgaaaa tcgacactga aaaatcgtcg
    33781 gtctttgatt atcgcggttt taatgggcga attggcgata gcgatatcca cggttctctg
    33841 gtctacacca ccggaaagcc gcgaccaaaa ctggaaggtg atgtcgagtc gcggcaattg
    33901 cggctggcgg acctgggacc gttgattggc gttgattcgg ggaaaggggc agaaaagtcg
    33961 aaacggtctg aacagaagaa gggcgaaaaa agcgttcagc ctgcgggcaa agtgctgcct
    34021 tatgaccgct tcgaaaccga taaatgggac gttatggatg ccgatgttcg cttcaaaggc
    34081 cgacgtatcg agcatggcag tagcctgccg attagcgatc tttctactca tatcatcctc
    34141 aaaaatgctg acctgcgcct gcaaccgctg aaatttggca tggcgggcgg cagcattgcg
    34201 gcgaatatcc atctggaagg cgataaaaag ccgatgcagg ggcgggcaga tattcaggct
    34261 cgtcgactga aactgaaaga actgatgccc gatgtggaac tgatgcagaa gacgctgggg
    34321 gaaatgaacg gtgacgcgga actacgcggt agcggtaact cggtggcggc gcttttaggc
    34381 aacagtaacg gcaacctgaa actgttgatg aatgacgggc tggtgagccg caacctgatg
    34441 gagattgttg ggctgaatgt cggcaactac attgtcggtg cgatatttgg tgacgatgag
    34501 gtgcgggtga actgcgcggc ggcgaatctg aatattgcca acggcgtagc gcgcccgcag
    34561 atttttgctt tcgatactga gaacgcgttg attaatgtta ccggcacggc aagttttgct
    34621 tcggaacagc tggatttgac tattgatccg gagagtaaag ggattcggat tatcacactg
    34681 cgttcgccgc tgtatgtgcg tgggacgttt aaaaatccgc aggctggggt gaaagccgga
    34741 ccgctgattg cccgtggtgc cgttgcggcg gcactggcaa cgctggtaac accggcggcg
    34801 gcgttattgg cactgatctc accttccgaa ggggaggcta atcagtgtcg gacgatattg
    34861 tcgcagatga agaagtgatg aggttggatt gtgtttgctg gtgagttcgg gagcagcgtg
    34921 ttcccctcac cctaatcctc tccccaaagg ggcgaggaga ctgttttgtg cgtgttgaaa
    34981 caacgagtac cgcttttccg ttcactttct gggggagggc tgttatgttc gcgatataac
    35041 atctcgctcc gcttttctcc ctctcccttt cagggagagg gctggggtga gggtggctgc
    35101 ccacacgata cttacaacga ctgatgttgc gtctcatggg tcagcagcag ggcgattaac
    35161 gtcaagccag ccatcgccgc cagatataac cccaccgcac ctaacccgta gttagtctgc
    35221 aaccaggctg cgatatacgg cgcaacggaa gccccgagaa tcgacgatac gttgtaagag
    35281 aacgatgctc cggtgtaacg cacttctgtc gggaacagct ctggtaacag cgcgcccatc
    35341 ggaccgaagg tcagccccat cagacttaac cccagcagca ggaaggcaaa aaccagaatc
    35401 gggttgccag aaccgagcaa agggttaaag gcgaatagcg cgaacaggat gagcagcgtg
    35461 gtgatgatta ccatgctttt acggcgacca aaggcatcag ccagtaatcc agcgactggc
    35521 accatcacgc caaaaccaat aactgccatc atcaacatcc acaacacttc gttacgcggc
    35581 aggccaagcc caactggcgc ggcggcggta ctaaaggtca tggagtagac cgtcatgatg
    35641 taaaacagcg tataggttgc cagcataatg aacgtaccca gtacggttac gcgaacatgt
    35701 ttggtcagca gcgtacccag cgggatcttc acctgttttt tcgctttagc gactttctca
    35761 aacaccggcg actcatgcag cgacacgcga acatacaggc cgataatgac cagcaccgcc
    35821 gagaagataa atggcacacg ccagccccag ctcataaact gctcgtcggt cagtagccag
    35881 gaaagcagca agaaagtgcc gttggcaaag aagaagccaa tcggtgcgcc cagctgcggg
    35941 aaggagccgt acaatgcacg tttgcgcggt ggggcgtttt cagtcgccag cagcgccgcg
    36001 ccgccccatt caccacctaa gcccagaccc tgaccaaatc gagccagcgc cagcagcagc
    36061 ggggcgaaaa taccaatcgt ggcatagcct ggcagcagac caatcaccac ggtcgaaatc
    36121 cccatcgtta gcaacgaggc gaccagcgtc gctttacgcc caacgcgatc gccaaaatga
    36181 ccaaaaacgg cagagccaat agggcgcgcg acgaaggcga tggcgaaggt ggcgagcgac
    36241 tgtagcgttg ctgccgtagg atcgccctgc gggaagaaga tatgcggaaa cacaataacg
    36301 gccgcagtgg cgtaaatgta aaagtcgaag aactcaatgg ctgtgccaat gagagaggcg
    36361 acaaggactt tattacgcga gttgatcggc gtgtattctt gctcgtggtc gagtgttgtg
    36421 gctgttgctt gcataatctt ttcttatttt ttggctaacg aatagccata ttacgcacag
    36481 caaaagcgat atttcaatgt gtaacaaatc gcggaaatgg tcaaaaacca ggcaaaaagg
    36541 ggataatgtt taggccagtg atttttcttc tatgaaatgc ttcacataat tttataaata
    36601 gtggataaaa ctggtttcag gttaaataaa agttaccaac tggatttata tgctggggtt
    36661 atgagttaga atgaaattgt ttgctcccaa atagatggct gaagaggctc tggggacgag
    36721 tttcaagagg ttatgtgcgc attatagccg gatgcgatgc tggcgcatct tatccggcct
    36781 acggggagcc aggccgaata aaatcgtatg ccggataagg cgtttacgcc gcatccggcc
    36841 tattttaagg ctgcgttttc cctggcattc gcgggtcgtc tttatattcg gcggtggcaa
    36901 tccacgccgc gcaaaacagc gtcaaacggg cgaagaagta gaaaaatgcc atcagcccca
    36961 gcacggaacc aaatgctgcg ccagaggggg atttcatcag cgatggcagg gtgtaggtca
    37021 tcacgatttt aatcacttca aaaccaatag cagccagaaa tgttccgcga atcagggctt
    37081 ttttgcgcgg acggtggcgc ggcaaccgcc agaagatcca gaagaaaagc agatagttgg
    37141 cgaagatgga aatcgccagg ccaatcaatc gccacgtcgg tttcagccac tcaatgctgt
    37201 tcaggtgcag ggcgctaata atcatttgct gcgccgaacc ggcaaccgag gtgatcgaaa
    37261 gcgtcacaat cagcgcaatc aacaaaccaa tcagtgaaat aaaatcacgc agatatttta
    37321 cccagaactt ctcctgatct tgcggcgagc gttcccagac atcacgcgac tgggcacgaa
    37381 tcgcttcacg caggttaccc atccagttga tgccggaata aagtgccacc gccaggccga
    37441 caagccctac agtcgtacgc tgctgaacgg cggtgttgat ggtgtttttc aacgtggcgg
    37501 ctagtgtcgg atcgctgatg ttttgcagaa ttttgtcgaa gatatcctgt agcaacatcg
    37561 gatgggaggc cagcacaaag ccccctgcgg caaacgacac catcaaaatc gggatcatcg
    37621 acaaaaatga gaaataggtg atagccgcac caaactggtt acccagccga tcgttaaagc
    37681 gttctgtcgc gcgaatcagg tgcgcaataa ccggttggcg ttggactttt tcggcagtgg
    37741 tggtgacggt ttccagcgcc tggctaactt tgccgccttt ctcgctatta tccaactgct
    37801 taatcggctc gtgctccaga tcctgggtgg gacgtttgat ctcgttttcc tgcgtcatcc
    37861 gctcttttgt ccttctgtcg tggtcattct taaaaagtat agtcggtcag tccaggtgct
    37921 ctttcattac gccagccagc cactgcataa acaggtttac acgccgggaa agctcccgac
    37981 gctgcgggta aaccagcgaa agggagagcg gctcggcacg gtagccaggt aatacttcaa
    38041 taagccgccc ggcacgcagg gcttcgcgca cggcaatgcg cgggatctga ataatcccca
    38101 gaccggcaag accggcggcg agatagtttt cgctactatt taccgtcaac atgccgccag
    38161 acttaaacca ctggacgcca ttaacgctgg caacctcaaa acctaacgga tgtacaccca
    38221 ggtgcggtgt ataacgcact attgcgtgtg aagtcagatc atcggggctt tgcggataac
    38281 caaagcgcgt cagatagtgc ggactggcac agttgaccat ggtcagtttg ccgaggggac
    38341 gggcgataac gccatcttcc ggtaacgcgc cagtgcgtat cacgcaatca aaaccatcat
    38401 gaagaatatc taccggacgg tcatggctac tcagttccag ctcaattccc ggatgcagat
    38461 agagaaattc cgacaggcgc ggcagtaaca ggtttttcgc gattcctggc gggatgtcga
    38521 tgcgtaattt accgctgata ctggtggcat cctgttgaaa cagaccgtcc agttcgttga
    38581 gattactcaa cacatctttt gcccgttgat aataggtcat gccttctggc gtgagtttga
    38641 cccgtcgcgt ggtgcgctgg agaagctggg tgccaagttg atgttccagt gcctgtatct
    38701 ggcgcgaaac acttcccttt ggcaaagcaa agaaatccgc tgcgcgggaa aaactttcca
    38761 gctccgcgac tttgatgaac aactgcattg cgtgaatttt atccatttct ttgaccgatt
    38821 gttgttcaca gcgaaacagt aacgcgcata tcgcattgtt tattgttttt tctaacatct
    38881 aatgccgggt ttcacaatgt tgtcatgacg aatgtggcga tttctcagca ggaaaagggc
    38941 ggaatagctt tatgagaaag gtgaatcccc ttaaacagag cgtatttttc agtgattccc
    39001 tttaaagtga atagaggacg tattggctat ttgtgcttat ttttgtctca ttgccatttg
    39061 ttattttggc ttgtagtcgc ggaatttcat ttttaattgc atgcgtacta accaccaata
    39121 taaggaaatc ctcaaaattg ttttaaatag tgccctcgac atgtaatttc aggcgtttag
    39181 ctgtaactat ttatatcggt aactattttc tacgcgtcat ctctgtagaa tggccaccgc
    39241 caaatggcga attcttattt tttctcgcgg gtaataccct tccactactt aagtcttttt
    39301 aacatattca ggtggagatt cagttatgca aatagtcatg tttgacaggc agtcaatatt
    39361 tattcatgga atgaaaatca gtttacagca gcgtattcca ggggtgagta ttcagggggc
    39421 cagtcaggca gacgagttat ggcaaaagct ggaaagttac cctgaagcct tagttatgct
    39481 cgatggcgat caggatggtg agttttgcta ctggttgctg caaaaaaccg tggtgcagtt
    39541 tcctgaggtt aaggtgttaa tcacggcgac ggattgcaat aaacggtggt tacaggaagt
    39601 tatccatttt aatgtgctgg ccattgtgcc tcgtgattca accgtcgaga catttgcgtt
    39661 ggcggtaaat agtgcggcga tggggatgat gtttctgcct ggagactggc ggacgacgcc
    39721 ggaaaaggac attaaagatc tcaaatcgtt aagcgcccgc cagcgggaga ttttaaccat
    39781 gttagcggcg ggcgagtcaa ataaggagat cggcagagcg ctgaatatca gtaccggaac
    39841 ggtaaaagcg catctggaat ccctgtatcg ccgtctggaa gtgaaaaatc gcacccaggc
    39901 ggcaatgatg ttaaatatct cctcctgaag aggataattt tacagccagc aggaagttag
    39961 cggctggctg taaaaatatt atggttcgcc gtacaaacca attaaacggc gtaccacacc
    40021 gttagtccag ccaaacccat cctgcaacgg atactcgcca ccgccgcctt cgcgtggaac
    40081 accatcggca atatggtatt tttcgatcag tttgtgctgt tccagataga actgattcac
    40141 cgtcttcagc cagcttcgcg cgatttcatc acccagaagg tcatcgccgt acattttaaa
    40201 tccctgaatc gccatccatt gtaacggtgc ccagccgttg ggtttatccc actgttcacc
    40261 ggtttcgtac tcgcttgcca gaatcccgcc aggtgtcagt aaccgactgc gcacggcatt
    40321 tgccagacga tcggcctgtt catggttcgc cataccgaca tagagtggca caatggcggc
    40381 agcggaaaac agcgccagtt gttcgcgtcg ccagtcgtaa tcgcggtaga tgccgttttc
    40441 atcatcccag aggtaacgct ttaccgcatc gcgacgggca ctggccttct ggcggaacag
    40501 tgcttctgtc tctttctcgc ctttcagcgc cgagatgttg gcgatggcgc tctccagttt
    40561 aaacaggaag gcattcagat cgatggggat gaactgggtg gtacgaatac tcgccagacg
    40621 accagtatca cgcagccaac gggaagagta atcccaaccg gaggccgccc ccgctcgtaa
    40681 atcgcggtac acctcgttgg gcgggcgacc agaatgtttc gcggtttcaa cgtcctcaag
    40741 ccaggattcg tcacgcggcg tgtcgcgatc gtcccagtaa cggttgagca gcgatccgtc
    40801 cggcatccgc acaacatggc gataggcctg attagggatc aacgattctg caccgtccat
    40861 ccagaaggca tattccattt tcaggtggtc gagatagcgg cgcgcaccgc gtacaccatc
    40921 ttcttcaaac aactccacca tcagcgcaaa aaccggtggt tgcgagcggc tcaaataata
    40981 ggtgcggttg ccgttgggga tgtgaccgta gttttcgatc atccaggcga agttatcggc
    41041 catgcatttc agcaaatctt cccgaccact ttccgccagc cccagcatgg tgaaatagga
    41101 atcccaatag taggtttcgc taaaacggcc gcccgggaca atatatgact gcggtagcgc
    41161 cagcagagaa gaccacggaa tgtgatcctg tggttcgcgg gttagcaccg gccacagctg
    41221 gtcgatatgc tctttcaggg aattttgcgg gtccgataca tactcgctgg agtagacctc
    41281 cggcagccag aagtgatttt caacaaactt gcgcaagtca aaatcacgat gacggcgcac
    41341 tttacggtag cggattaaaa tatccagcgg gtccattttg ggtgcgcagt cgggaaaggt
    41401 tttactgtcg gggaaaattt tcgccgactg aacatgctcg aacagttcga gatagcgatc
    41461 ggcaggcgtc agcgcatcag aggcgggcag cccttcaatc atctcgggtt ccggttctgc
    41521 ctcgatcatc tcatccagtt ttaattcata cgggtccagt tcatagcaga gatcgacttc
    41581 gatcatcagt tcgtctggat tagggttttg aattttctga ttgagcataa ggatccaacc
    41641 tccgaaattc gtcgctaatt atcggctttt ctttaagggt agtcattcgc tccgcctggt
    41701 gggcgacaaa gcgtgcggtg gatcacgttt attaacttgt caaaaaatat aacgcactaa
    41761 tatcaatgga atttataaaa atacggattt ggtgttgttt ttcattttca aaacattcaa
    41821 tcctgctctc gccgcaatca tgatatgcca ttcctgatct gggtaatagg cacaggctat
    41881 cttattgata gtttatattc atgtaattga ttgattccta tcaaaaagcg tatctccctg
    41941 ttttttatga tggctttgtc tcgcagagac gtttgcagca ttctggaaaa ttttctgggt
    42001 cacaataact caccaccgtg agggaagaat aatgaaaatg gtctcacgta ttaccgcgat
    42061 cggcctggct ggcgtcgcga tttgctattt agggttatct ggttatgtgt ggtaccacga
    42121 taataaacgc agtaaacagg ccgatgttca ggcatctgct gtcagtgaaa ataataaggt
    42181 tttaggcttt ctccgcgaaa aaggatgcga ctattgccac acgccttcgg cagaattacc
    42241 cgcctattat tatattcctg gcgcgaaaca gttgatggat tacgacatta agcttggata
    42301 taaatctttt aacctcgagg ccgtgcgtgc ggcactgctg gctgataaac ccgtttcgca
    42361 aagcgatctg aataagattg aatgggtgat gcagtatgaa actatgccac caacgcgtta
    42421 taccgcgcta cactgggcgg gtaaggtgag tgatgaagag cgggcggaaa tactggcctg
    42481 gattgcaaaa cagcgcgcgg aatattacgc cagcaatgat actgctccgg agcatcgcaa
    42541 tgaaccggtg cagcccatcc cgcaaaaact gcctaccgat gcgcaaaaag tggcgttggg
    42601 ctttgcgctg tatcacgatc cccgtttatc ggctgatagc accatttcat gcgctcattg
    42661 ccatgcgttg aatgcggggg gcgtcgatgg cagaaaaaca tcgattggtg ttggtggcgc
    42721 agttgggccg attaacgcgc cgacggtatt taactcagta tttaacgttg agcagttctg
    42781 ggatggtcgt gcggcaacat tgcaggatca ggcaggtgga ccgccgttga acccgattga
    42841 aatggcgtcg aaatcctggg acgaaattat tgctaagctg gaaaaagatc cgcagcttaa
    42901 agcgcagttc ctcgaagttt atccgcaagg tttcagtggc gaaaatatta ctgatgccat
    42961 tgctgaattt gagaaaacat taattacgcc ggattcccca tttgataaat ggttgcgtgg
    43021 agatgaaaat gcgctgacgg cgcaacagaa aaaaggctat caattattta aagataataa
    43081 atgtgcaact tgtcatggtg gtattattct cggcggacgt tcctttgaac cgttggggct
    43141 gaaaaaagac tttaactttg gggaaattac ggcggcggat attggtcgta tgaatgtgac
    43201 taaagaagag cgtgataaat tgcgccagaa agtacccggt ttacgtaacg ttgctttaac
    43261 ggcaccgtac ttccatcgcg gtgacgtgcc gacgctggac ggggcggtga aactgatgct
    43321 gcgctatcag gtaggcaaag agctgccgca ggaggatgtg gatgatatcg tagctttcct
    43381 gcacagtctg aacggggtgt acacgccgta tatgcaggat aaacaataac taatttgatc
    43441 gcccgaacag gaatgtttgg gcgattttta ttacgataat aaagtctgtt tttaatatta
    43501 tcatgttaaa tgtttatatt ataaaaagtc gtttttctgc ttaggatttt gttatttaaa
    43561 ttaagcctgt aatgccttgc ttccattgcg gataaatcct acttttttat tgccttcaaa
    43621 taaatttaag gagttcgaaa tggaccagaa gctgttaacg gatttccgct cagaactact
    43681 cgattcacgt tttggcgcaa aggccatttc tactatcgcg gagtcaaaac gatttccgct
    43741 gcacgaaatg cgcgatgatg tcgcatttca gattatcaat gatgaattat atcttgatgg
    43801 caacgctcgt cagaacctgg ccactttctg ccagacctgg gacgacgaaa acgtccataa
    43861 attgatggat ttgtcgatca a
//
LOCUS       DAZPEB010000008        43379 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZPEB010000008 DAZPEB010000000
VERSION     DAZPEB010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43379
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.008"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            164..421
                     /locus_tag="ACHURJ_000437"
                     /pseudo
     CDS             164..421
                     /locus_tag="ACHURJ_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
     gene            complement(471..1727)
                     /gene="nupG"
                     /locus_tag="ACHURJ_000438"
     CDS             complement(471..1727)
                     /gene="nupG"
                     /locus_tag="ACHURJ_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFR1279516.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(1929..3008)
                     /gene="mltC"
                     /locus_tag="ACHURJ_000439"
     CDS             complement(1929..3008)
                     /gene="mltC"
                     /locus_tag="ACHURJ_000439"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFR1279517.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(3073..3348)
                     /locus_tag="ACHURJ_000440"
     CDS             complement(3073..3348)
                     /locus_tag="ACHURJ_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFR1279518.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(3376..4428)
                     /gene="mutY"
                     /locus_tag="ACHURJ_000441"
     CDS             complement(3376..4428)
                     /gene="mutY"
                     /locus_tag="ACHURJ_000441"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFR1279519.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            4589..5308
                     /gene="trmB"
                     /locus_tag="ACHURJ_000442"
     CDS             4589..5308
                     /gene="trmB"
                     /locus_tag="ACHURJ_000442"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFR1279520.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLNNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            5308..5634
                     /locus_tag="ACHURJ_000443"
     CDS             5308..5634
                     /locus_tag="ACHURJ_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFR1279521.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            5818..6537
                     /locus_tag="ACHURJ_000444"
     CDS             5818..6537
                     /locus_tag="ACHURJ_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFR1279522.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            6713..7759
                     /gene="ansB"
                     /locus_tag="ACHURJ_000445"
     CDS             6713..7759
                     /gene="ansB"
                     /locus_tag="ACHURJ_000445"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFR1279523.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            7876..8883
                     /locus_tag="ACHURJ_000446"
     CDS             7876..8883
                     /locus_tag="ACHURJ_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFR1279524.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQSAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(9038..10174)
                     /gene="hemW"
                     /locus_tag="ACHURJ_000447"
     CDS             complement(9038..10174)
                     /gene="hemW"
                     /locus_tag="ACHURJ_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFR1279525.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRAEFSAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(10167..10760)
                     /locus_tag="ACHURJ_000448"
     CDS             complement(10167..10760)
                     /locus_tag="ACHURJ_000448"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFR1279526.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            complement(10768..11058)
                     /gene="yggU"
                     /locus_tag="ACHURJ_000449"
     CDS             complement(10768..11058)
                     /gene="yggU"
                     /locus_tag="ACHURJ_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFR1279527.1"
                     /translation="MNAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            complement(11055..11621)
                     /gene="yggT"
                     /locus_tag="ACHURJ_000450"
     CDS             complement(11055..11621)
                     /gene="yggT"
                     /locus_tag="ACHURJ_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFR1279528.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNILLPGMWMAL"
     gene            complement(11639..12343)
                     /gene="yggS"
                     /locus_tag="ACHURJ_000451"
     CDS             complement(11639..12343)
                     /gene="yggS"
                     /locus_tag="ACHURJ_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFR1279529.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            12361..13341
                     /locus_tag="ACHURJ_000452"
     CDS             12361..13341
                     /locus_tag="ACHURJ_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFR1279530.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDALDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRHGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVGEGRL"
     gene            complement(13517..13933)
                     /gene="ruvX"
                     /locus_tag="ACHURJ_000453"
     CDS             complement(13517..13933)
                     /gene="ruvX"
                     /locus_tag="ACHURJ_000453"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFR1279531.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(13933..14496)
                     /locus_tag="ACHURJ_000454"
     CDS             complement(13933..14496)
                     /locus_tag="ACHURJ_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFR1279532.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTASADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            complement(14605..15555)
                     /gene="gshB"
                     /locus_tag="ACHURJ_000455"
     CDS             complement(14605..15555)
                     /gene="gshB"
                     /locus_tag="ACHURJ_000455"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFR1279533.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVLLMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            complement(15568..16299)
                     /gene="rsmE"
                     /locus_tag="ACHURJ_000456"
     CDS             complement(15568..16299)
                     /gene="rsmE"
                     /locus_tag="ACHURJ_000456"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFR1279534.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            complement(16379..17086)
                     /gene="endA"
                     /locus_tag="ACHURJ_000457"
     CDS             complement(16379..17086)
                     /gene="endA"
                     /locus_tag="ACHURJ_000457"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFR1279535.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            complement(17181..17678)
                     /locus_tag="ACHURJ_000458"
     CDS             complement(17181..17678)
                     /locus_tag="ACHURJ_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFR1279536.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MENVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            complement(17755..19149)
                     /gene="galP"
                     /locus_tag="ACHURJ_000459"
     CDS             complement(17755..19149)
                     /gene="galP"
                     /locus_tag="ACHURJ_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFR1279537.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            complement(19585..20739)
                     /gene="metK"
                     /locus_tag="ACHURJ_000460"
     CDS             complement(19585..20739)
                     /gene="metK"
                     /locus_tag="ACHURJ_000460"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFR1279538.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATTFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            20795..21046
                     /gene="yqgD"
                     /locus_tag="ACHURJ_000461"
                     /pseudo
     CDS             20795..21046
                     /gene="yqgD"
                     /locus_tag="ACHURJ_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
     gene            21157..21300
                     /gene="yqgG"
                     /locus_tag="ACHURJ_000462"
     CDS             21157..21300
                     /gene="yqgG"
                     /locus_tag="ACHURJ_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFR1279539.1"
                     /translation="MNRSLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            21394..21525
                     /gene="yqgB"
                     /locus_tag="ACHURJ_000463"
     CDS             21394..21525
                     /gene="yqgB"
                     /locus_tag="ACHURJ_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFR1279540.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            21534..23510
                     /gene="speA"
                     /locus_tag="ACHURJ_000464"
     CDS             21534..23510
                     /gene="speA"
                     /locus_tag="ACHURJ_000464"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFR1279541.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            23647..24567
                     /gene="speB"
                     /locus_tag="ACHURJ_000465"
     CDS             23647..24567
                     /gene="speB"
                     /locus_tag="ACHURJ_000465"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFR1279542.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(24773..25531)
                     /gene="loiP"
                     /locus_tag="ACHURJ_000466"
                     /gene_synonym="yggG"
     CDS             complement(24773..25531)
                     /gene="loiP"
                     /locus_tag="ACHURJ_000466"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFR1279543.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDFLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            25801..27792
                     /gene="tkt"
                     /locus_tag="ACHURJ_000467"
     CDS             25801..27792
                     /gene="tkt"
                     /locus_tag="ACHURJ_000467"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFR1279544.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIIGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            28106..28549
                     /gene="cmtB"
                     /locus_tag="ACHURJ_000468"
     CDS             28106..28549
                     /gene="cmtB"
                     /locus_tag="ACHURJ_000468"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFR1279545.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISHR"
     gene            28577..29965
                     /gene="cmtA"
                     /locus_tag="ACHURJ_000469"
     CDS             28577..29965
                     /gene="cmtA"
                     /locus_tag="ACHURJ_000469"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFR1279546.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSLGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSTNAVKAMKQEGAFSLSRVKRIAFVCDTGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            29980..31257
                     /locus_tag="ACHURJ_000470"
     CDS             29980..31257
                     /locus_tag="ACHURJ_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFR1279547.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLVEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVDLAASKGIELVYVNTKGMSDPVQTLRALTGDVGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPDTVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGIDIGEAAQ"
     gene            31254..32219
                     /gene="glpX"
                     /locus_tag="ACHURJ_000471"
     CDS             31254..32219
                     /gene="glpX"
                     /locus_tag="ACHURJ_000471"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFR1279548.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGVIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQESKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            32241..32750
                     /gene="fumE"
                     /locus_tag="ACHURJ_000472"
     CDS             32241..32750
                     /gene="fumE"
                     /locus_tag="ACHURJ_000472"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFR1279549.1"
                     /translation="MAKLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQFWHYLNEQD
                     ETPGFADDMTWDFISNVNSITRNAMLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTILKELTP"
     gene            32747..33460
                     /locus_tag="ACHURJ_000473"
     CDS             32747..33460
                     /locus_tag="ACHURJ_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFR1279550.1"
                     /translation="MKIELTVNGLKIQAQYQNEEIENVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(33432..34109)
                     /locus_tag="ACHURJ_000474"
     CDS             complement(33432..34109)
                     /locus_tag="ACHURJ_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1279551.1"
                     /translation="MLTLNQISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(34103..34780)
                     /locus_tag="ACHURJ_000475"
     CDS             complement(34103..34780)
                     /locus_tag="ACHURJ_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1279552.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(34768..35475)
                     /locus_tag="ACHURJ_000476"
     CDS             complement(34768..35475)
                     /locus_tag="ACHURJ_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFR1279553.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLRILVIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            complement(35476..36054)
                     /locus_tag="ACHURJ_000477"
     CDS             complement(35476..36054)
                     /locus_tag="ACHURJ_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFR1279554.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(36077..36508)
                     /locus_tag="ACHURJ_000478"
     CDS             complement(36077..36508)
                     /locus_tag="ACHURJ_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HFR1279555.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYARQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            36880..37899
                     /gene="epd"
                     /locus_tag="ACHURJ_000479"
     CDS             36880..37899
                     /gene="epd"
                     /locus_tag="ACHURJ_000479"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFR1279556.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            37949..39112
                     /gene="pgk"
                     /locus_tag="ACHURJ_000480"
     CDS             37949..39112
                     /gene="pgk"
                     /locus_tag="ACHURJ_000480"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFR1279557.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            39327..40406
                     /gene="fbaA"
                     /locus_tag="ACHURJ_000481"
     CDS             39327..40406
                     /gene="fbaA"
                     /locus_tag="ACHURJ_000481"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFR1279558.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLSFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(40470..40568)
                     /locus_tag="ACHURJ_000482"
                     /pseudo
     CDS             complement(40470..40568)
                     /locus_tag="ACHURJ_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            40764..41624
                     /gene="mscS"
                     /locus_tag="ACHURJ_000483"
     CDS             40764..41624
                     /gene="mscS"
                     /locus_tag="ACHURJ_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFR1279559.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            41763..42387
                     /gene="argO"
                     /locus_tag="ACHURJ_000484"
                     /pseudo
     CDS             41763..42387
                     /gene="argO"
                     /locus_tag="ACHURJ_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
     gene            42480..43220
                     /locus_tag="ACHURJ_000485"
     CDS             42480..43220
                     /locus_tag="ACHURJ_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFR1279560.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
BASE COUNT        10605 a        10827 c        11557 g        10390 t
ORIGIN      
        1 atctaacccg acgtacaatg tgttattgtt gttcatgctg gcctccgtgg tggaaatacc
       61 ggggtgtagc aacagctcac ccatgcggct ctgacaacga tgttaacccg cgttaaatat
      121 cccacgttgg ccagcatctg tctcacggaa agtcatactg ccttatattc gcggtgaagt
      181 ggagttggtg cggattcgtg atgccgaagg gcgaattgcg gcagaagggg cgttgcctta
      241 tccacctggc gtgctttgcg tggtacccgg ggaagtctgg ggtggggcgg ttcaacgtta
      301 tttccttgca ctggaagaag gggtaaattt gttgccggga ttttcgccgg agctgcaagg
      361 tgtttatagc gaaaccgatg cggatggcat gaaacggttg tacggttatg tgttgaagta
      421 agaataaaaa aaacgggtca ccttctggcg acccgttttt ctttgcgtaa ttagtggcta
      481 accgtctgtg tgcctgtcgg gacacgaacg tgtttatatt tgaacatcgc catgaacgcg
      541 aaggccagaa ccacggagta accagcgaaa atcaaccata cggtctgcca gtcggtaatg
      601 ccgttttggg tgtacatctc aacaacttta ccgctcacga tgccgccgag gatacagccg
      661 aagccgttag tcatcatcag gaacatccct tgtgcactgg cgcgaattgc cgggctaact
      721 tctttttcga caaacaccga accagagatg ttgaagaagt cgaatgcgca accgtaaacg
      781 atcatcgaca gtaccagcag tacagtaccg aacggagtcg ggtcgccgta agcaaacagc
      841 gcaaaacgca ggatccacgc cacaatactg atcatcatta cgttcttaat accgtagcgg
      901 cttaagaaga acgggatggt cagaatgaac agggtttcag agatctgcga aatcgacatg
      961 atgattgacg catgctgcac aataaagctg ctggcaaaca tcggatcttt gtcgaagctg
     1021 tgcaggaagg tattaccgaa catgttggta atctgcagtt ccgcgcccag cagcattgag
     1081 aagataaaga agattgccat acgcttgttt ttaaacagcg cgaatgcatc gaggcccagc
     1141 agggttgtcc agctctgatt cgcttgctgt ttagcaaccg gaatatgcgg cagagtcagg
     1201 gtaaacagaa ccagaatggc ggaaagtgct gcgccaatat acagctgcat gtggcttaat
     1261 tcgaagccag acaggctcac cacccacatt gccatgataa agccgatggt gccccagata
     1321 cggattggcg ggaagtcagt aacgatatcc atcccggcat tttgcaggcg atagtaagag
     1381 atggtgttga ttaacccaag cgttggcata taagcaaacg agttaatcaa tatcacaagg
     1441 aacatcgcct ccggtgtcgt gacctgtgcc gccatgaaca gcgtgatagc gccaatggtg
     1501 tggcaaatgg catataccca tttcgcactt aaccatttgt cggccacaat ccccagcagc
     1561 gcaggcataa agaccgctgc gatacccagt gagctataaa ctgcgccaat agaagcaccg
     1621 tcaaacttca gggtaacaaa catataggag ccgagggtcg tcagccaact tccccacaga
     1681 cagaactgca gaaaagagag gattttcagc tgcagcttaa gattcatgtt aatttcctca
     1741 catcgtgatg cggatgagtg tttgaaaaag cacatttgac cgtgcgagat gatgggattc
     1801 tatcaacttc gcggactggt tttttgttac ctgtggcaaa taattgcaaa cattcccaat
     1861 ttgcaaaacg aaattgtgat gtggataaca tttttgcccc tgagcatcgt caggggcggt
     1921 taatggaatt atcggcggcg gtaggatttt tgcgcggtat tcactttata aagataacgg
     1981 cgagattccg cagagggatg gcgggtcgtc agcgtctgat aaacatcgcc cggcgtcatg
     2041 gtgttaataa tattggcagc ctgaatctta tcattcgaaa agactcgcag cacgctgcct
     2101 gcgccgccgt tataggcggt gatgacggca taacgccgcg atgttgggtt atcaattccg
     2161 ccgagataaa cattgttcag catcgccaga tacgcggtgc cggtatcaat attgctggca
     2221 ggatcaaaca agaaactgcg gctcggcgtg ccggatttcc cctgcgagcg gaacacatct
     2281 ttcccggcag tatgttgtac cacctgcatt aatcccagcg catcggaacg gctgaccgca
     2341 tacgggttaa aggaagattc ggtctgcata attgccagaa tcagcgactc atcaacgcca
     2401 tatttccgtg acgcctggcg gaccatgccg agatatttgt gcgcacgttt atcaaggtgg
     2461 ttcggtacca tgttaatggt gacgctgtag atgatacgca gcccgttact gcggctttgc
     2521 agacggtttt tcagcagata atccgcgaag ttgcttgcgc gaccttccca gcgaatcggc
     2581 tgcccggtgt tgtccaccac ctgaccgtaa aggaaaggtt ctttcgaaat cgtaatatca
     2641 tcaacgtcgg aatagagatc gaccgaactc ggatcgtcac ccatcagcaa cgttttgata
     2701 attgcccggc gcaaatgcgc ggcaggttct gtcccggcga tagtttcgat agtaatcgta
     2761 ccgtcatcga agttgatgtg gctgcgggtc tgatattgat cggtgtattt cacgtagtcc
     2821 ttaggaccgg cgatcaccac ctctttgaat ccccagatgt tctcaatatt gtgggcaaat
     2881 tgccccatca gaatatcaaa accgttggta tctttgaccc aggcttcgtt ataggtatcg
     2941 ccttttttgg tcgtcgaaca ggagatgagc aacggcgcaa tcaaagccag cgcgagatat
     3001 tttttcatca ttccgggtgc gtgttgtgtt tgtttttatg caagttgccg gaggcgtgct
     3061 ccggcactgt ttttattttt tatcttccgg cgtatagccc tcgatatgca cctctttacc
     3121 ctcgaacagg aagttgacca tctcctgctc aagcagcttg cggtgctcgg cattcatcat
     3181 gttgagtttc ttttcattaa tcagcatggt ttgcttgtgc tgccactgcg cccaggcttc
     3241 tttggagatc tcgttataga tgcgttttcc cagctcgccg gggtacagct gaaaatcctg
     3301 accttctgct tcacgttgca ggaaagtaca aaaaatcgtt ctgctcataa atcatcctct
     3361 ttatcgactc acgcgctaaa ccggcgcgcc agtgcgtaac tgctgtaaca aacgctccac
     3421 gggagccgcc aggccaactg acggcggttg cgctaagtta taccagagcg cattgccttc
     3481 atccatgcag ccggtgaatg acgacacggg aagccacata ggcacaatat ctaagtggaa
     3541 atggctgaag gtatgccgaa acgcggtcag ttgcgtcagg ttatcggcag caatctgccg
     3601 ttgcgccagc cactgccgca aactttcttc gtcggcaaac tgcgggaaac agtataaacc
     3661 gccccacaat ccgctcggcg gacgctgcgc cagcaatact tcatcttcgt gctgtaacag
     3721 caaaaagtag ccggtgcgct ccggcagcgt ctgtttcggt tttttgcccg gataaagcga
     3781 ccagctatta ttggcggcgg caatacatcc gttttgtagc ggacagagcg aacatttcgg
     3841 cttcgagcgc gtacaaatca tcgcgcccaa atccatcatc gcctgattaa accgttccac
     3901 gccgaccgcg ggcgtcacct gctcgctcaa actccataat ttattctcga cctctttttt
     3961 cccaggccag ccgcttacag catagcagcg cgccagcacg cgtttgacgt taccgtcgag
     4021 aatcggaaag tgcttaccca gagaaagcga gagaatcgcg cctgcggtgg aacgcccgac
     4081 gcccggtaac gccgcgactt cttcaaaggt ttccgggaat ttaccgccgt gtaaggtcgc
     4141 cacttgttgt gccgctttat gcagattgcg cgcgcgggcg taatagccaa gcccggtcca
     4201 caagtggaga acttcgtcga gcggcgcatt ggcgagatcg gtcaccgtcg ggaagcgcgc
     4261 cataaagcgt tcaaaatagg ggataacggt cgcaacctga gtttgttgca acatcacttc
     4321 tgagagccat actttgtagg gcgtcttgtc aatttgccag ggcagcgttt ttcgcccgta
     4381 tttatcgtac cagtccagaa cctgggctga aaattgcgac gcttgcatgg tcaccgaatt
     4441 cactgttgtt gggggcaaga ttgcagcaca gcgacggcag ggtgtaaacc ggaactttcc
     4501 gcagccacgg ccttatcatg cttgcatccg gcaactaact ttggataatg cccgttttca
     4561 gaacactttc acaagcgact aaatctttat gaaaaacgac gtcatttcac cggaatttga
     4621 tgaaaacggc cgcccactgc gccgtatccg tagttttgtg cgccgccagg ggcgactgac
     4681 caaaggccag gaacatgcgc tggaaaacta ctggccggtg atgggcgttg agttcagcga
     4741 agatatgctg gatttccccg cgctttttgg ccgtgaagcg ccggtgacgc ttgagattgg
     4801 ttttggcatg ggggcgtcgc tggtggcaat ggctaaagat cgccctgagc aggacttcct
     4861 cggcattgaa gtgcattcac cgggcgttgg tgcgtgcctg gcttctgcgc atgaagaagg
     4921 tttaaacaac ctgcgagtga tgtgtcacga tgcggttgaa gtgctgcata aaatgattcc
     4981 tgacaattca ttgcgcatgg tgcagctctt tttccctgac ccgtggcaca aagcgcgcca
     5041 taataaacgc cgtatcgtac aggtgccgtt tgccgaactg gtaaaaagca aactgcagct
     5101 ggggggcgta ttccatatgg cgaccgactg ggaaccttat gcggaacata tgcttgaagt
     5161 gatgtcttct attgacggtt ataaaaacct gtcagagagc aatgattacg taccgcgtcc
     5221 ggcatcacgt ccggtgacga aatttgaaca acgtggtcat cgtcttggtc acggagtatg
     5281 ggacttaatg ttcgagaggg tgaaataatg gcaaagaacc gtagccgtcg tctgcgtaaa
     5341 aaaatgcaca tcgacgaatt ccaggaatta ggattttcgg tggcatggcg attcccggaa
     5401 ggtacatcgg aagaacagat tgataaaacc gttgatgatt ttattaacga ggttatcgaa
     5461 ccgaacaaac tggcctttga cggcagcggt tatctggcct gggaaggtct gatctgcatg
     5521 caggaaatcg gcaaatgcac cgaagaacat caggcgattg tgcgtaagtg gctggaagag
     5581 cgcaaactgg atgaggtacg caccagcgaa cttttcgacg tttggtggga ctaagaaagc
     5641 atacgggcga tgacaaatgc aaaactgcct gatgcgctac gcttatcagg cctggaaaga
     5701 tgcacgatcg agtaggcggg ataaggtgtt tacgccgcat ccggcatgga aaacgcgtac
     5761 tttgttatca atctggggcc agcaaatgct ggcctgattt tttcttgagg gaagactatg
     5821 atgcgcaaaa tgctgctggc ggcagcactt tcagtgacgg caatgaccgc tcacgccgac
     5881 taccagtgca gcgtcacgcc gcgtgacgat gtgattgtca gcccgcaaac cgtgcaggtg
     5941 aagggcgaaa acggcaatct ggtgatcacg ccagacggca acgtgatgta taacggtaag
     6001 caatattccc tgaatgccgc ccagcgcgag caggcgaagg attatcaggc tgaactacgc
     6061 agcacgctgc cgtggattga tgaaggcgcg aaaagccgcg tcgaaaaagc ccgtattgcg
     6121 ctggataaaa ttatcgttca ggagatgggc gaaagcagca aaatgcgcag ccgtctgacc
     6181 aaacttgatg cgcaactgaa agagcagatg aaccgcatta tcgaaacgcg cagcgatggc
     6241 ctgacgtttc actataaagc cattgatcag gttcgcgccg aaggccagca attagtgaat
     6301 caggcaatgg gtggaatttt acaggacagc attaatgaaa tgggcgcgaa agcggtgctg
     6361 aaaagcggcg gtaacccatt acagaatgtg ctgggaagcc tgggggggct gcaatcctca
     6421 atccaaaccg agtggaaaaa gcaggaaaaa gatttccagc agtttggcaa agatgtttgt
     6481 agccgcgttg tgactctgga agatagccgc aaagccctgg tcgggaattt aaaataatcc
     6541 tctattttaa gacggcataa tactttttta tgccgtttaa ttcttcgttt tgttacctgc
     6601 ctctaacttt gtagagctcc aaaatatatt cacgttgtaa attgtttaac gtcaaatttc
     6661 ccatacagag ctaagggata atgcgtagcg ttcacgtaac tggaggaatg aaatggagtt
     6721 tttcaaaaag acggcacttg ccgcactggt tatgggtttt agtggtgcag cattggcatt
     6781 acccaatatc accattttag caaccggcgg gaccattgcc ggtggtggtg actccgcaac
     6841 caaatctaac tacacagcgg gtaaagttgg cgtagaaaat ctggttaatg cggtgccgca
     6901 actaaaagac attgcgaacg ttaaaggcga gcaggtagtg aatatcggct cccaggacat
     6961 gaacgataat gtctggctga cactggcgaa aaaaattaac accgactgcg ataaaaccga
     7021 cggcttcgtc attacccacg gtaccgacac gatggaagaa accgcttact tcctcgacct
     7081 gacggtgaaa tgcgacaaac cggtggtgat ggtcggcgca atgcgcccgt ccacgtccat
     7141 gagcgcagac ggtccattca acctgtataa cgcggtagtg accgcagctg ataaagcatc
     7201 cgctaatcgt ggcgtgctgg tggtgatgaa cgacaccgta ctggacggtc gcgatgtaac
     7261 caaaaccaac accaccgacg tagcgacctt caagtctgtt aactacggtc ctctgggata
     7321 cattcacaac ggtaagattg actaccaacg taccccggca cgtaagcaca ccagcgatac
     7381 gccattcgat gtctctaagc tgaatgagct gccgaaagtc ggcatcgttt ataactacgc
     7441 taacgcatcc gatcttccgg ctaaagcact ggtagatgcg ggctatgatg gcatcgttag
     7501 cgctggtgtg ggtaatggta acctgtataa atccgtgttc gacaccctgg caaccgccgc
     7561 gaaaaacggc actgcagtag tgcgttcttc ccgcgtaccg acgggtgcta ccactcagga
     7621 tgctgaagtg gatgatgcga aatacggctt cgtcgcctct ggcacgctga acccgcaaaa
     7681 agcgcgcgtc ctgctgcagc tggctctgac gcaaaccaaa gatccgcagc agatccagca
     7741 gatcttcaat cagtactaat cacctcgctc cggtatcgtg ccggggcttt ttcacttcag
     7801 acttacgtcc attgccaatt ttcattaccc taatgataat caccggaata aattatttcg
     7861 cgtgaggttt ttcgggtgaa aaagcaatgg attgttggca cggcactgct tatgttgatg
     7921 actggtaatg cctgggcaga tggcgaaccg ccaactgaaa atatcttaaa agatcaattc
     7981 aaaaagcagt atcacggcat tctcaagctt gatgctatca ccttaaaaaa tcttgatgct
     8041 aagggtaatc aggccacctg gtcagcggaa ggcgatgtct cttccagtga cgatctctat
     8101 acctgggtcg gtcagttggc agattacgaa ctgctcgaac agacctggac gaaagataaa
     8161 ccggtaaaat tctcggcgat gttaaccagt aaaggaacgc ccgcgtctgg ctggtcggtg
     8221 aacttttact cttttcagtc ggcagccagc gatcgtgggc gggtggtcga cgatatcaaa
     8281 acgaataata aatatctgat cgtgaatagc gaagatttca attatcgctt tagtcagctt
     8341 gaatctgcgt tgaataccca gaagaactcg attcctgcgc tggaaaagga agtgaaagcg
     8401 cttgataagc aaatggtggc ggcgcagaaa gcagcggatg catattgggg aaaagatgca
     8461 aacggtaagc aaatgacccg cgaagaagcc tttaaaaaaa ttcaccaaca gcgtgatgag
     8521 tttaataaac agaacgatag cgaggcgttc gcggttaaat atgacaaaga ggtttatcaa
     8581 ccggcgattg cggcatgtca taaacagagt gaagagtgtt atgaagtgcc gattcagcag
     8641 aaacgagatt tcgatatcaa cgaacaacgg cgacagactt ttctgcaatc acaaaaactc
     8701 agccgtaaat tgcaggatga ctgggtaacg ctcgaaaaag ggcaatatcc gttaacgatg
     8761 aaagtttcag aaataaacag taaaaaagtc gcgattttga tgaaaattga cgatatcaac
     8821 caagctaatg aacgctggaa aaaagatacc gaacaactgc ggcgcaatgg ggtgattaag
     8881 taagtttgat atagtgcacg atttgtagca ggatgcggcg ttgaccccgc atccggcaat
     8941 caacgtctga tgcgacgctt gcgcgtctta tcagacctac aaccgttgcc acatgtaggc
     9001 tggataaggc gttcacgccg catccggcaa tacaagttta ctcagccaga aaaagctcca
     9061 gcagcgaatt taaaaacagc ttcccatgtt ccgttatctg ccagtaatcc gcacattctg
     9121 tgagataacc ctgggcaatc gcctcgtcta actgtgggcg aatcacatct tcgcaaagcc
     9181 cggtatacgc actaaactct gcgcgtggcg cggcctccag caagcgaaag cggttcataa
     9241 agaactcaaa cggcttatct gcggcttcga catcacgctg gctttccaga taccttcctt
     9301 gcataaaacc acgaggatga cgcgttttag tggtacgcag aatgcgccca tccgggaagg
     9361 tgactttgcc gtgcgcgccg cagccaatac cgatgtagtc accaaatcgc cagtagttga
     9421 gattgtgctg gcactgataa ccgggtttgg cgtaagcgga agtttcatat tgctgataac
     9481 ccgctgcggt taataactga tgcccctgtt cgaaaatatc ccacagcgcg tcatcgtccg
     9541 gcagcaccgg tggtcgcgaa ccaaacagcg tattgggttc gatggtcagt tgataccagg
     9601 aaagatgcgg cggattcagt tcaatggcct ggcgtagatc gccaagcgcc tcttccagtg
     9661 attgatccgg tagtccatgc atcaaatcaa ggttaaagct acgtaaccct aaaccgctcg
     9721 ccagctttgc cgctcgtttc gcttcttgcg ggccatgaat gcgcccaagt cgtttcagct
     9781 tttcttcgct aaaactctgc acaccaatag agatgcggtt cacaccagca cgctgataat
     9841 cgacaaagcg atcggcttct accgtaccag ggttcgcttc catagtaatt tctgcatccg
     9901 ctgccagcgg caaacgcgca cgcacgccgt ccagcagcgt ttgcatcgcc gggccggaaa
     9961 gcaggctcgg cgtaccaccg ccaataaaga ttgtctttac ttcacggccc tgagcgtaag
    10021 ccacatcatt gtccagatcg caaagcagat gctgaacata atcgtcgtgc ggcacttctc
    10081 ctttcaacgc gtgagagttg aaatcgcagt acgggcattt ctgcacgcac cacgggatgt
    10141 gaatgtagag actcagcggc ggtaatttaa ccattacgta aagcgtccag cagcagtttc
    10201 aacgcctgac cacggtggga aatggcgctc ttttcttcgc gggtcagttc ggcggcggtt
    10261 ttcccttcgg aaggtacgaa gaagattgga tcataaccaa agccaccagt gcccgctggt
    10321 tcgcgagtaa tcacgcccgg ccagctgccg tggcatacca gcggagtcgg atcttccgcg
    10381 tgacgcagat ataccagcac gcagtggaaa cgtgcctgac gttggtcgtc cggtacgtct
    10441 ttcagtgttt ccaacagttt ttgcagattc ttttgatcgg tcgcgtcttc accggaataa
    10501 cgcgcggagt aaatacccgg cgcgccaccc agcgcatcta ccgccagacc agagtcgtcg
    10561 gcaattgccg gtaaaccggt cacttttgcc gcatggcgcg ctttcagaat cgcgttttcg
    10621 ataaaggtta ggccggtttc ttcagcggaa tcaacgccga ggtctgtttg tgccacgata
    10681 tcaagaccaa agtcgctaag cagcgacgcc agctcacgca ctttaccggc attgccggtt
    10741 gcgaggacaa ctttttgcat aggataccta attaattaac gccgcgattt ctggcgggat
    10801 ttgttgcgga ttaatgattt taatttgttt gtggcggcca agttcgcctt tttcaatcac
    10861 cacctggctt ttggcaaccc ggaattgctt accgagaaac ttcaccagat gactgttggc
    10921 ctggccgtca actggcggcg cggtaatggc gactttaact tcgtcgccat gtaaaccgac
    10981 aatagaatca cggctggctt tcggctgaat atagagccgt aaaaccagac cgtcatcatt
    11041 aactgttacg gcattcataa cgccatccac atccccggca gcaggatgtt tccggttgcc
    11101 tgtaatactt ctgcgacacc catattgatg acatacagca gcagaacgag gatcatcggc
    11161 gagaaatcaa tcccacccat tgccggtagc aagcggcgaa tcgggcgcag cagcggatcg
    11221 gccagctgaa tcagcacgta ttcaatcggg ctacgaccct ggcttaccca gctcataatc
    11281 gccatcacca gcaggaccca gaaaatcagc aagccgatgg ttttcagcag aatcagtaaa
    11341 ccggcaatcc agatgattgg caggaaggtc accactttaa acagcacaat ggctttgata
    11401 aaactgagaa tataggcaac cagcagcgag gcgctgtcaa tgggacccat tgccggaata
    11461 acgcggcgca gtggcccgat aattggctgc gttaccttca ctacaaactg tgagaagggg
    11521 ttgtaaaaat cacaatgagc ccactgcatc cagatgcgta ataacagcac catggtatac
    11581 agctcaatga ccgttgaaag caggaaagtc aacgtattca tggcgttcct ttaattcctt
    11641 attttttaga gtaatcacgc gcaccaaaaa ttgcagtgcc gatacgaacc atcgtgctac
    11701 ctgccgcaat ggcggcttcc atatcgtccg acattcccag agagagcgtg tcgatatgcg
    11761 ggtagcgcgt tttcagtccg gcaaatgcta cagccatttg gcgtgcaact tcaaactgcc
    11821 ttacatattc tgactcaggc gcagggattg ccatcagccc gcgcagacgt aaacgcggta
    11881 gttcagcgac cgccgctgcc agctcgtcca gttctgccag ttgaatcccg gacttactgt
    11941 tttcatcact aatattaatt tgaatcagta cgttaagagg gggaagttct gccgggcgct
    12001 gatcgttgag gcgggtagca atgcgcaaac ggtcgatggt atgacaccag tcgaaatgct
    12061 ctgccaccag gcggctttta ttagactgca acgggccaat aaaatgccat tctaatcctg
    12121 ttacgcccag ttcctgaaag tggcgaattt tatctacccc ttcctgaacg tagttttcac
    12181 caaattgacg ctgcccggca tcaatggctt ctgcgatggc gctcgcaggt tttgttttac
    12241 tgactgcaag cagcgtaatt tcttctggag aacggccgca acgcgttgca gccgctgaga
    12301 ttttgtcccg gacctgtgcc aggttatgcg caatatcgtt cattttccga ggatcttagt
    12361 atgaatatgg aagaaattgt ggcccttagt gtaaagcata acgtctcgga tctacacctg
    12421 tgcagcgcct ggcccgcacg atggcgcatt cgcgggctaa tggaagctgc gccgtttgat
    12481 gcgctggacg tcgaagagct actgcgggag tggctggatg acgatcagcg ggcaatattg
    12541 ctggaaaatg gccagctgga ttttgccgtg tcgctggcgg aaaaccagcg attgcgtggc
    12601 agcgcattcg cgcaacggca tggcatttcg ctggcgttac ggctgttacc ttcgcactgc
    12661 ccgcagctcg aacagcttgg cgcaccaacg gtattgccgg aattactcaa gagcgagaat
    12721 ggcctgattc tggtgacggg ggcgacgggg agcggcaaat ctaccacgct ggcggcgatg
    12781 gttggctatc tcaatcaaca tgccgatgcg catattctga cgctggaaga tcctgttgaa
    12841 tatatctatg ccagccagcg atgtttgatc cagcagcggg aaattggttt gcactgtatg
    12901 acgttcgcat cgggattgcg ggccgcattg cgggaagatc ctgatgtgat tttgctcgga
    12961 gagctgcgtg acagcgagac aatccgtctg gcactgacgg cggcagaaac cgggcatttg
    13021 gtgctagcaa cattacatac gcgtggtgcc gcgcaggcag ttgagcgact ggtggattca
    13081 tttccggcgc aggaaaaaga tcccgtacgt aatcaactgg caggtagttt acgggccgtg
    13141 ttgtcacaaa agctggaagt ggataaacag gaaggacgcg tggcgctgtt tgaattattg
    13201 attaacactc ccgcggtggg gaatttgatt cgcgaaggga aaacccacca gttaccgcat
    13261 gttattcaaa ccgggcagca ggtggggatg ataacgtttc agcagagtta tcagcagcgg
    13321 gtgggggaag ggcgtttgtg acgttgccat gcccgatgcg caagtttata gggcctataa
    13381 aggcaataaa tgtctgatgc gacgctaacg cgtcttatca gacctacagc cgctgccaga
    13441 tgtgtgctgg ataaggcgtt cacgccgcat ccggcaagat ggcggtaacg actatccgcc
    13501 aggcttttag atcgccttaa tatccctgct cgaaatagct ttcgagaata ataaccgcag
    13561 aggcagagtc gactttgcct ttgttgagcg cccgatagcc gccctgttca aacagaccgg
    13621 aacgggcttc cacggtgcta agacgttcgt catggagctt tacttcaaca ccgaaacgac
    13681 catgaatacg gttggcaaat ttacgcgctc tggcagtcaa tggttgctcg gtgccgtcca
    13741 tattcagcgg caaaccgacg atgatttcgt ccggctgcca ctctttcagt aaacgttcga
    13801 tgaggttcca gtccggcgta ccgtcctgtg ctttaattgc aggcaaaggg cgagcagtgc
    13861 cggtaatgcg ttggccgacc gctacgccaa tgcttttggt gccgaagtcg aaggcgagta
    13921 aggttccact catcaggcgt gccctgccac accaggcatg gtgagaatat ccacaccaat
    13981 cagttttgcc gcctcgcgcc agcggtcggc aatcggcgtt ttgaacagaa tattcagatc
    14041 cgccgaggcc gttagccacg cgttatcgag aatttcttgt tccagttgac ctttttccca
    14101 ggaggcatag cccagagcca ccaatacgtc agacggttgt ttatcggtgc cgagcgtttc
    14161 cagcacatcg cgggaagtgg tcatcaccgt gttgtctgaa atgcgaatgc tggaagcaaa
    14221 attggagggc ggagtatgca aaataaaccc gcgatcttca gccagtgggc cgccgagcat
    14281 aaccggttta tccagacgga ttgattcatc acgcggctcc ggcgtgatct tcagcttttc
    14341 cagaatccct tcaattttga gattttccag cggcttgttg acgatgatcc ccattgcacc
    14401 attggtatta tgttcgcaaa tgtagaccac ggaacgacgg aaaatcggat cctggagagc
    14461 aggcatggca ataagaaagt gatgctgtaa attcattgtc agaggttctg tttcctggtt
    14521 caaaaaagca acagcgccca gtatgcgtgg aaagaagggc gctgtcactc agagtctcaa
    14581 cgagatcctt ctcgctaagg tgggttactg ctgctgtaaa cgtgcttcga tggcatccat
    14641 taacattccg gtgatcgaca ccggaaactc tgcttcaatc tcacgaatac aggttgggct
    14701 ggtgacgtta atttcagtca gacggtcgcc gatgatatcc agaccaacaa aaatcagccc
    14761 tttttctttc agcgtcggcc cgatctgacg ggcgattttc cagtcacttt ccgtcagcgg
    14821 ccgaggttca ccgcgaccac cggcagccag attgccacgg gtttcgcccc cctgcggaat
    14881 acgcgccagg cagtacggta ccggctcgcc atccaccacc agcacgcgtt tgtcgccatc
    14941 tttaatggct ggcaggtaat tttgcgccat gcagtagcga gtgccatgct cagtcagggt
    15001 ttcggcaatc acgccgaggt ttggatcgcc ttctttcacg cggaaaatcg acgcgccgcc
    15061 cataccgtcc agcggcttaa gaatgatgtc gctgtgtttc tcccagaacg cttttagctg
    15121 cgctttattg cgcgtaacca gcgtttctgg cgttaagtca gagaaccagg cggtaaacag
    15181 tttctcgtta cagtcgcgca ggctctgcgg cttgttaacg atcagcgtcc ctttctcttc
    15241 ggcacgttcc agaatatagg tcgcgtagat aaactcggta tcaaacggcg ggtctttacg
    15301 catcaggagc acatcgagat cggccagcgg cagatcctgt tcaccgacga acgaaaacca
    15361 ctcttcgtag ttctgcttca cgttcagcgt gcgggtatgg gcgcgagctt cgccattgat
    15421 cagatacaga tcgcccatct ccatatagtg aagttcgtaa ccacgacgct gtgcttccag
    15481 caacatagcg aaactggaat ctttcttgat gttgatgttt gcgatggggt ccatcacgat
    15541 gccgagcttg atcattattc ttctccgtta gcccaaatcg ccaaatcgta cttgtagcgc
    15601 ggtaatggcg gtgagcgcag ttgtctctgt acgcaaaacg cgaggtccca acaggatatc
    15661 agtaaattga tagcgggcag tcatggcaat ttcatctgcc gataaaccgc cttccgggcc
    15721 aatcagcagg cggacgcgtt caaccggtaa cggcaacgta ttgatgctgt tactggcgcg
    15781 cgggtgaaga ttcagtttca gtccttcatc ctgctctgca caccaggctt ccagatccat
    15841 cgctggacgg atttccggca cccggttacg accacactgc tcacaggcag caattgcaat
    15901 cttctgccac tgctggagct tcttgttcag acgttcacta tccagtttaa cgccgcagcg
    15961 ctcagaaaaa agtggcgtaa tgaggcttac accgagttcg atcgatttct ggatcgtaaa
    16021 ttccattttt tcaccacgcg acatcacctg accgaggtga atatgcagcg gagattcgcg
    16081 atcgtcgatc ttgccttcca gcaccttcac ttccacgctt tttttgctgg cgctggtaat
    16141 ttcggcgtca aagacctggt tgctaccgtc aaacaattgc aacgcctgcc ccggccccat
    16201 gcgcagtacg cgcccgatat ggttggcggc atcttcgcaa agcgcgatgt gagaatggct
    16261 ggtcagtggt tcaggatgat aaatgcgggg gatacgcata gttaaaaatc cgcgtcgtct
    16321 ccccacgcgg ttgtacgcgt ggggtagggg ttaacaaaaa gagtcccgct agtgtaggtt
    16381 agctctttcg cgcctggcaa gcgcgttgca catacgggtt atgattgcct tgcaccttcg
    16441 cgatgcgttc atcgcgctcg cactcccagt cggtaaccgg atacatcttg ttccatgcgt
    16501 tgaacagctg cgtttgctgg cgagagagtg tcaggttgta ttggtcgcgc atatagaagt
    16561 aggtgcgcgc aatggcaccg cgtgcacgcg ctggtggttc ggcagctttt tctttgaaat
    16621 cgaccttcat ggcgcattga ccgtactggc cttcgccgcc attccactgg ctgtacataa
    16681 agttgccgcg atcgccattc acctcaccga ctgacggctg caggttatgc atatcgcttt
    16741 ccatcttgcg atagaccgga tctttagcgc agtttttacg tccaccgtcc tgccagcact
    16801 ggcgctggtg accgaactgc caggcgggaa cgacgtgttc ccactctacg cggctggctc
    16861 ggttttcatt tttgcgcacc tgatagccgc acgattgcag atcaacaacg ccttttttgc
    16921 cctgccagtt aattttacat ccgcaataaa acgtaccggg cgcgtcagcg tggactttta
    16981 ccgccgcggc tttcgcctga gaaaaactat tgataccttc ggccaacgcc gggccggaaa
    17041 atgctgcgct cagtaccacc gcagcaatag acaaataacg gtacatcact tactccgtgt
    17101 taaaacgagc cagcaacgta gcgaaagttg ttttggtatg caatcagcca gatcaggaaa
    17161 gttcctgata gttcagatgg ttattttgcg accagttgtt cgccgcagtg aacacagcga
    17221 tagacggctt cgccacgaac tacgcgatta tggcggcgta cggtaagctg atgctcctgg
    17281 cacttgcagc ggtaggggaa ggtgttgcga cgcacggatt gcagttcgaa ctgatgcgta
    17341 cgacgggcgg gaacacccag cacgttttcc atcatccact tccactcttt gccatgtggt
    17401 gctacgcgcc cgaaatgttt ccataccagc aaatgtgcca gttcgtgcgg caccacttct
    17461 tcaataaagg cttcactgtt ttccaacagc aaaacgggat tgaggcggat ttcatagctt
    17521 tccagccagg ctgttccggc ggaggtgccg cgctgggtat aagagagttt tggctccggg
    17581 tagttacgcc ccaatttcag gttagcctgg gcgagttttt cccgcaggcg acgcataacg
    17641 gcctgttgga tggcgatagg gagacgggat gttttcatag cggcagagga tagagcgaag
    17701 aaaagatgac tgcaagaggt ggcttcctcc gcgatgggag gaagcttggg gagattaatc
    17761 gtgagcgcct atttcgcgca gtttacgacc tttcatcaga ttacgttcaa tatgttccag
    17821 cgaaacgtgt ttggtttccg gtaccagcca caatgtcagc aggataaaca gtacgttcag
    17881 agccgcatac acccagaagg tgttggcgtt acccagtgtg ttgagcatgg tcaggaacgt
    17941 tgcgccaacg atcatgttgg caatccagtt ggtggcggta gagcaggtga tgccaaaatc
    18001 gcggcctttc agcggctgaa tttcggagca cagtacccaa atcagcggac cggcactcat
    18061 ggcaaaaccg acaataaaca tcagcagcat ggcgatggcg aaatactgcg ctgacggaga
    18121 gtgaataccg atatgcatca ttgtaccgag tacgcccatg ccagcagcca tcaccaggaa
    18181 gcccagcgtt agcgttggtt tacgtcccca gcggtcaaca aggccgattg cgataaaggt
    18241 ggcaagtacg ttggtcaggc cgacaatcac tgtcccccac atttgctcgg tagtgttggt
    18301 ataacccgcc agttcgaaga ttttcggcgc gtaatacatg atgacgttca tcccggtgaa
    18361 ttgctgcatt acctgtaaca gtacgccaag gaacaccgcg cggcggaagt tgctattctc
    18421 tttaaacagc gcccagccac tctgtttaac ctgcaaactt tcacggattt catccagttc
    18481 gcgtttcgct tccgcgctgg tgtcacgcag gcgtagcagc acgcgttcgg catcaacaaa
    18541 acggcgtttg gcggcaaacc aacgtgggct gtctggcagg aagaagacac caatcagcag
    18601 caaaattgcc gggatgataa tcacacccag catccagcgc catgcaccgg tgtagctgaa
    18661 ggcggtatca gaaagataag caccgaggat cccgatagtg atcatcaact gatacatcga
    18721 gatcatactg ccacgaattt tttccggcgc aatttcagag aggtacagcg gtgcggtata
    18781 agaggccaca cccaccgcca gccccagtag aacgcgggaa agaatcagta cttcaacgtt
    18841 tggcgcagcc gcagagaaca gcgaaccggc aacaaacaaa attgcgccga tcatcaggct
    18901 ctttttgcgc ccgagtttaa aggagagcca gccgctgccc accgcaccga ctgccgcacc
    18961 gaacatcatg gagcttacga cccattcttg cgtgtgcgaa gtaatctgga attcatctgc
    19021 aataaacggc agtgcgccag caattacacc gatatccagg ccaaagagta atcccgccag
    19081 agcggcaagg aagcagacga aaaacgtcat tgccttgttt gaccgcccct gttttttagc
    19141 gtcaggcatg atgccctcca atatggttat tttttattgt gaattaagat aggtgagtac
    19201 gacgtaaaaa gatgtgaagc aaatcacatc agtgtaatcg cttacactaa catttatctt
    19261 agttatggtt taagtgcatg aaaataattg agttatatca tcatagatta aacgctgtta
    19321 tctgcaatta agactttact gaaaagaaat gtaacaactg tgaaaactga ataataatat
    19381 gaaaatatta caaatatttg gctggtgtta ttggtgtaat cggtttcagt agtgatgata
    19441 atttgcgata aaacgtgttg aggaagaatg atgttattgt gcgggcagct ttttgccgga
    19501 tgcggcgtga atgcctatcc accgcggtga ggtaaaagaa aaaggccagc gcgaggctgg
    19561 cctttgaaag caggtgaaga aagattactt cagaccggca gcatcgcgca gcagctgcgc
    19621 tttgtcggtt ttttcccacg ggaaatgttc acgaccaaag tgaccgtatg ctgcggtttc
    19681 tttgtagatc gggtgcagca gatccagcat ctgaatcaga ccgtatgggc gcaggtcgaa
    19741 gaactcacgt accagcagag tcagttgttc agaaggcact ttctcagtac cgaaagtttc
    19801 taccatgatg gaggtcggtt cagccacgcc gattgcgtag gaaacctgaa tttcacaacg
    19861 atcggccagg ccagcagcaa cgatgttttt cgcgacataa cgtgctgcgt aggctgcgga
    19921 acggtccact tttgatggat ctttaccaga gaatgctccg ccaccgtgac gtgccatgcc
    19981 gccgtaggta tcaacgataa ttttacgacc agtcagaccg cagtcaccca tcgggccacc
    20041 gataacaaaa cgaccggtcg ggttgatgaa gaatgtggtg gcagaagtca gccattcagc
    20101 gggcagaatt ggcttgatga tctcttccat taccgcttct tgcagcgatt tctggtcgat
    20161 ctcttcagag tgctgagtgg aaagcacgac agcatcaata ccaacgattt tgccgtcgtc
    20221 atactggaaa gtcacctggc ttttcgcgtc cgggcgcagc cacggcagag tgccgttttt
    20281 acgcacttca gcctgacgct gtaccagacg gtgtgcatag gtgataggtg ctggcatcag
    20341 cacgtcggtt tcattagttg cgtagccaaa catcagaccc tggtcacccg cgccctgttc
    20401 cagcggatcg gcacggtcaa cgccctggtt gatgtcagga gactgtttgc cgatagcgct
    20461 cagaaccgca caggagttag cgtcaaagcc catgtcggaa tgcacatagc caatttcgcg
    20521 aacggtgtta cgagtgatct cttcgatgtc tacccaggcg ctggtggtga tttcgccgcc
    20581 aactaaaacc atgccggttt ttacgtaggt ttcgcaagca acgcgtgctt tcggatcctg
    20641 ttcgaggatc gcgtctaaaa cggcatcaga gatttggtca gcaattttgt caggatgccc
    20701 ttcagagacg gactcggacg taaaaaggtg ttttgccata tttaatatca cctaaagaga
    20761 atttggttag ctcaaactgt tgtgtggatt ttctgtggta gcggatccta ccacgactct
    20821 gcaggttaaa aacactggca gtctgagtgt taatcggtat ggatggatta acatctagat
    20881 ggctatttta ggtcaattct tcacccgatt tccacttttt tttgaatcgt gtctcattct
    20941 gttaaaaacg tggctggaaa ttttttctga caatgccggc attctgcgta tttatatttt
    21001 gcaattttct gccattgtgg ggtataaaac gcggcgcgcg gcttaaataa aaagcacacg
    21061 acgtttcttt cgtgttgcca cttccagccg ggttgaaatc agagttttgg cttgtgggtt
    21121 cgtcttaaca ggcggccgtg gaggtgatac gaaataatga accgtagtct gctgcttaac
    21181 ctgtctcacc gttctggtga agattcgttc cccgcactct gcatctctgc tttgcatacc
    21241 tgccgatgtt atacccatct cggcgcttct caggattcaa gagctggtta cagttactga
    21301 ggactgaaca agggcgctct tgtaaaaaca agagttttct cgtggtttcg ccgaactgtc
    21361 acacttacgt tcggttatgt gcttaataat gttatgaaaa agaaaccggt tgcgcagttg
    21421 gagcgtcagc attcactgct ggaaaatcca tgtgcttatg ggttgttatc gcagttccag
    21481 gctgcgatag tcgttaactg ttttacactt aataaaataa tttgaggttc gctatgtctg
    21541 acgacatgtc tatgggtttg ccttcgtcag cgggcgaaca cggtgtacta cgctccatgc
    21601 aggaggttgc aatgagctcc caggaagcca gcaagatgct gcgtacttac aatattgcct
    21661 ggtggggcaa taactactat gacgttaacg agctgggcca catcagcgtg tgcccggacc
    21721 cggacgtccc ggaagctcgc gtcgatctcg cgcagttagt gaaaactcgt gaagcacagg
    21781 gtcagcgtct gcctgcactg ttctgtttcc cacagatcct gcagcaccgt ttgcgttcca
    21841 ttaacgccgc gttcaaacgt gcgagggaat cctacggcta taacggcgat tacttccttg
    21901 tttatccgat caaagttaac cagcaccgcc gcgtgattga gtccctgatt cattcgggcg
    21961 aaccgctggg tctggaagcc ggttccaaag ccgagttgat ggcagtgctg gcacatgctg
    22021 gcatgacccg tagcgtcatc gtctgcaacg gttataaaga ccgcgaatat atccgcctgg
    22081 cattaattgg cgagaagatg gggcacaagg tctatctggt cattgagaag atgtcagaaa
    22141 tcgccattgt gctggatgaa gcagaacgtc tgaatgtcgt tcctcgtctg ggcgtgcgtg
    22201 cacgtctggc ttcgcagggc tcgggtaaat ggcagtcttc cggcggggaa aaatcgaagt
    22261 tcggcctggc tgcgactcag gtactgcaac tggttgaaac gctgcgtgaa gctgggcgtc
    22321 tcgacagcct gcaactactg cacttccacc tcggttcgca gatggcgaat attcgcgata
    22381 tcgccacggg cgttcgtgaa tccgcgcgtt tctatgtgga actgcacaag ctgggcgtca
    22441 atattcagtg cttcgacgtc ggcggcggtc tgggcgtgga ctatgaaggc acccgttcgc
    22501 agtctgactg ctccgtaaac tacggcctca atgaatatgc caacaacatc atctgggcga
    22561 ttggtgatgc gtgtgaagaa aacggtctgc cgcatccgac ggtaatcacc gaatcgggtc
    22621 gtgcggtgac tgcgcatcac accgtgctgg tgtctaatat catcggcgtg gaacgtaacg
    22681 aatacacggt gccgaccgcg cctgcagaag atgcgccgcg cgcgctgcaa agcatgtggg
    22741 aaacctggca ggagatgcac gaaccgggaa ctcgccgttc tctgcgtgaa tggttacacg
    22801 acagtcagat ggatctgcac gacattcata tcggctactc ttccggcacc tttagcctgc
    22861 aagaacgtgc atgggcagaa caactctatc tgagcatgtg ccatgaagtg cagaagcaac
    22921 tggatccgca aaaccgtgcg catcgtccga taatcgacga actgcaggaa cgtatggcgg
    22981 acaaaatgta cgtcaatttc tcgctgttcc agtcaatgcc ggacgcatgg gggatcgacc
    23041 agttgttccc ggttctgccg ctggaagggc tggatcaagt gccggaacgt cgcgctgtgc
    23101 tgctggatat tacctgtgac tctgacggtg ctatcgacca ctatattgat ggtgatggta
    23161 ttgccacgac aatgccaatg ccggagtacg atccagagaa tccgccgatg ctcggtttct
    23221 ttatggtcgg cgcatatcag gagatcctcg gcaacatgca caacctgttc ggtgataccg
    23281 aagcggttga cgtgttcgtc ttccctgacg gtagtgtaga agtggaactg tctgacgaag
    23341 gcgataccgt tgcggatatg ctgcaatatg tgcagctcga tccgaaaacg ctgttaactc
    23401 agttccgcga tcaagtgaag aaaaccgatc ttgatgctga actgcaacaa cagttccttg
    23461 aagagttcga ggcaggtttg tacggttata cttatctgga agatgagtaa gtccgtatta
    23521 cttgaatccg cttaatttag cggtgataat ccgccacaat ttattgtgac aaatccaacc
    23581 cttcctcgtc gggcctaacg acgcggaagg gtttttttat atcgactttg taataggagt
    23641 ccatccatga gcaccttagg tcatcaatac gataactcac tggtttccaa cgcctttggt
    23701 tttttacgcc tgccgatgaa cttccagccg tatgacagcg atgctgactg ggtgattact
    23761 ggtgtgccgt tcgatatggc cacttctggt cgtgcggggg gacgccacgg tccggcagcg
    23821 atccgtcagg tttcgacgaa tcttgcctgg gaacacaatc gcttcccgtg gaatttcgac
    23881 atgcgtgagc gcctgaacgt cgtggactgc ggcgatctgg tatatgcctt tggcgatgcc
    23941 cgtgagatga gcgaaaaact gcaggcacac gccgagaagc tgctggctgc cggtaagcgt
    24001 atgctctctt tcggtggtga tcactttgtt acgctgccgc tgctgcgtgc tcatgcgaag
    24061 catttcggca aaatggcgct ggtacacttt gacgcccaca ccgataccta cgcgaacggt
    24121 tgtgaatttg accacggcac catgttctat accgcgccga aagaaggtct gatcgacccg
    24181 aatcattccg tgcagattgg tattcgtacc gagtttgata aagacaacgg ttttaccgtg
    24241 ctggacgcct gccaggtgaa cgatcgcagc gtggatgacg ttatcgccca agtgaaacag
    24301 attgtgggtg atatgccggt ttacctgacc tttgatatcg actgcctaga tcctgctttc
    24361 gcgccgggaa ccggtacgcc agtgattggc ggcctgacct ccgatcgcgc cattaaactg
    24421 gtacgcggcc tgaaagatct caacattgtt gggatggacg tagtggaagt ggctccggca
    24481 tacgatcagt cggaaatcac cgctctggct gcggcgacgc tggcgctgga gatgctgtat
    24541 attcaggcgg cgaaaaaggg cgagtaagca ccagatgcga tgcgcacggg taaaacgtgc
    24601 cattaatgtc ggatgcggcg tgaacgcctt atccgaccta cgttcggcac ccgtaggccg
    24661 gataagatgc gccagcatcg catccggcaa tgcgcacaag gtaacaaatg tgccattaat
    24721 gtcagatgcg gcgtgaacgc cttatctgac ctacgttcga caccaccagg ctttacttaa
    24781 tcccatccgc gctcatgcga tcgcgaatat gctgggcgcg ttcggcggat gccggatggt
    24841 cgtcaaacat tgagctttgg cggccttctt ccagtttcgc cagtttttca aagctggtgg
    24901 caagacctgc cgggctgatg ccgcgttggc gcagaaaatc gtaagaataa tcatcggctt
    24961 ctgcttcctg gcgctgggag aattgcgaat tgactaattt ctcgcccaga tcaccaagtt
    25021 gtgattgaga taaactgccg acaatcccgc ccgcagaggc cgcagctact cgcacggcat
    25081 ttgtaccaag cgccacctgc atccctttct tcacatggcc taacgccacg tgccccatct
    25141 cgtgaccgat caccgcttcg acttcgttat ccgtcatcat atccatcagc ccgctataga
    25201 cgcggataca gccgttagcc attgcaaagg cgttcacatc cttcgccata tacactttgt
    25261 aattcaccgg ctgaccgttg atattgttgc ctagcgcatt ggcaatagtt gtcagacgtt
    25321 tagcgtattc gctattggct ggcgcaatcg tcgccttgct gtccatctcc tgacatgcct
    25381 gatcgctcag ggttttcacc tgcgcatcac tcaaactgta agcctgaaaa gcttccgctc
    25441 ctgatgagag cagtccgttg gagtccatat tctggcaacc agtcagtacc gttgccacgc
    25501 tcattgctac caataaggcg cgaattttca tgttttctct tccgcacaag gtcaggatta
    25561 ttctaaaaat gcgccgtttg caggtgaatc gacgctcagt ctcagtataa ggaaaagcgc
    25621 agcggacggg cgagtagatt gcgcaacatg cgagcatgat ccagagattt ctgaagcggc
    25681 aaaaggatgt tccatgtaca tgacgcgcgg cttgcggtaa attgttggca aattttccgg
    25741 cgtagcccaa aacgcgctgt cgttaagggc gtgcccttca tcatccgatc tggagtcaaa
    25801 atgtcctcac gtaaagagct tgccaatgct attcgtgcgc tgagcatgga cgcagtacag
    25861 aaagccaaat ccggtcaccc gggtgcccct atgggtatgg ctgacattgc cgaagtcctg
    25921 tggcgtgatt tcctgaaaca caacccgcag aatccgtcct gggctgaccg tgaccgcttc
    25981 gtgctgtcca acggccacgg ctccatgctg atctacagcc tgctgcacct caccggttac
    26041 gatctgccga tggaagaact gaaaaacttc cgtcagctgc actctaaaac tccgggccac
    26101 ccggaagtgg gttacaccgc tggtgtggaa accaccaccg gtccgctggg tcagggtatt
    26161 gccaacgcag tcggtatggc gattgcagaa aaaacgctgg cggcgcagtt taaccgtccg
    26221 ggccacgaca ttgtcgacca ctacacctac gccttcatgg gcgacggctg catgatggaa
    26281 ggcatctccc acgaagtttg ctctctggcg ggtacgctga agctgggtaa actgattgcg
    26341 ttctacgatg acaacggtat ctctattgat ggtcacgttg aaggctggtt caccgacgac
    26401 accgcaatgc gtttcgaagc ttacggctgg cacgttattc gcgacatcga cggtcatgac
    26461 gcggcatcca tcaaacgcgc agtagaagaa gcgcgcgcag tgactgacaa accgtccctg
    26521 ctgatgtgca aaaccatcat cggtttcggt tccccgaaca aagccggtac tcacgactcc
    26581 cacggtgcgc ctctgggcga cgctgaaatt gccctgaccc gcgaacagct gggctggaaa
    26641 tatgcaccgt tcgaaatccc gtctgaaatc tatgctcagt gggatgcgaa agaagcaggc
    26701 caggcgaaag aatccgcatg gaacgagaaa ttcgctgctt acgcgaaagc ttatccgcag
    26761 gaagccgctg aatttacccg ccgtatgaaa ggcgaaatgc cgtctgactt cgacgcgaaa
    26821 gcaaaagagt tcattgctaa actgcaggct aacccggcga aaatcgccag ccgtaaagcg
    26881 tctcagaatg ctatcgaagc tttcggtccg ctgttgccgg aattcctcgg cggttctgct
    26941 gacctggcac cgtctaacct gaccctgtgg tctggttcta aagcaatcaa cgaagatgct
    27001 gcgggtaact acatccacta cggtgttcgc gagttcggta tgaccgcgat tgctaacggt
    27061 atctccctgc acggtggttt cctgccgtac acctccacct tcctgatgtt cgtggaatac
    27121 gcacgtaacg ccgtacgtat ggctgcgctg atgaaacagc gtcaggtgat ggtttacacc
    27181 cacgactcca tcggtctggg cgaagatggc ccgactcacc agccggtaga gcaggtcgct
    27241 tctctgcgcg taaccccgaa catgtctaca tggcgtccgt gtgaccaggt tgaatccgcg
    27301 gtcgcgtgga aatacggtgt tgagcgtcag gacggcccga ccgcactgat cctctcccgt
    27361 cagaacctgg cgcagcagga acgaactgaa gagcaactgg caaacatcgc gcgcggtggt
    27421 tatgtgctga aagactgcgc cggtcagccg gaactgattt tcatcgctac cggttcagaa
    27481 gttgaactgg ctgttgctgc ctacgaaaaa ctgactgccg aaggcgtgaa agcgcgcgtg
    27541 gtgtccatgc cgtctaccga cgcatttgac aagcaggatg ctgcttaccg tgaatccgta
    27601 ctgccgaaag cggttactgc acgcgttgcg gtagaagcgg gtattgctga ctactggtac
    27661 aagtatgttg gcctgaatgg cgctatcatc ggtatgacca ccttcggtga gtcagctccg
    27721 gcagagttgc tgttcgaaga gttcggcttc accgtcgaca acgtcgttgc gaaagcaaaa
    27781 gaactgctgt aattagcatt tcgggtaaaa aggtcgcttc ggcgaccttt tttattacct
    27841 tgatatgtcc gtttgcggac aagcaataga taaagcgtgt tgcagatcac aaatatttat
    27901 atgcaataaa tatcaattat gtaatatgca tcacgatatg cgtattgaca tttgttgtta
    27961 tatctataac tcaatgttat ataagaaatt aataattcac tgttttcaaa acaccggttt
    28021 ccctgctcaa ttgctttcat taaaccgcac gagtatttta tttttaccgc cggacggtac
    28081 cctaaaaata aaataaggat aatttatgcg gcttagtgac tattttccag aatcatcaat
    28141 ctcagttata cattcagcaa aagattggca ggaagctatc gatttctcga tggtatcatt
    28201 gctggataaa aactatatca gcgagaatta cattcaggca attaaagatt ccaccattaa
    28261 caatggccct tattatattc tcgcaccagg cgtggcaatg cctcatgcgc gaccggaatg
    28321 tggggcgctt aaaaccggga tgtctttgac attacttgaa caaggtgttt attttccggg
    28381 gaatgacgaa ccaattaaat tactcatcgg actctctgct gccgatgccg attcgcacat
    28441 tggcgctatt caggcgttaa gtgaattact gtgcgaagaa gaaatactcg aacaactctt
    28501 aacagcatca tcagaaaaac aattagcgga cattatcagc cacagataat tactctctcc
    28561 tctacaggat aatattatgg aaaacaagtc tgctcgtgca aaggtccagg cttttggggg
    28621 ctttttgact gcaatggtca tccccaatat tggtgctttt attgcctggg gttttattac
    28681 tgcgttattt attcccaccg gttggctgcc taacgaacat ttcgccaaaa ttgtcggccc
    28741 gatgattacc tatttattgc ccgtgatgat tggttctaca ggtggtcatc tggtcggcgg
    28801 taaacgcggg gcggtcatgg gcggaatagg tactattggt gtgatcgttg gcgcagagat
    28861 cccgatgttc cttggctcaa tgatcatggg gccgctcggt gggttggtca taaaatatgt
    28921 cgataaggca ctggaaaaac gcatacctgc cggttttgag atggttatca ataacttctc
    28981 attaggtatc gcggggatgc tcctttgtct gctgggtttt gaagtgatcg gcccggcggt
    29041 gttaattgcc aatactttcg tcaaagagtg tatcgaggcg ctggtacatg cgggttatct
    29101 gcctctgctg tcagtcatca atgaaccggc gaaagtgctt ttccttaata atgcgatcga
    29161 tcagggcgtc tattatccgc tgggaatgca acaggcttcg gttaacggta aatccatctt
    29221 ctttatggtg gcctctaacc caggtccggg cctggggctg ctgctggcgt ttaccttgtt
    29281 tggtaaaggg atgagtaaac gttctgcgcc cggggcgatg attattcact tcctcggtgg
    29341 gatccacgaa ctgtatttcc catatgtgct gatgaagccg ctgactatta ttgccatgat
    29401 tgcgggcggt atgtctggta cctggatgtt taacttactg gacggtggtc tggtggctgg
    29461 cccaagtctg gggtctatct ttgcttacct ggcactgacg ccgaaaggct cgttcctggc
    29521 gacaattgcc ggtgttacgg taggtaccct ggtgtccttt gctattactt cgctgatact
    29581 gaagatggaa aaaacggtgg aaacggagag cgaagatgag tttgctcagt caaccaatgc
    29641 ggttaaggcg atgaaacaag agggtgcatt ctcgttaagc agggttaagc gtatcgcctt
    29701 tgtttgcgat acggggatgg gctccagtgc gatgggcgcg accaccttcc gtaaacgcct
    29761 ggaaaaagcg gggctggcaa tagaagtaaa acattacgcg atagaaaacg tgcctgcgga
    29821 tgccgatatc gtcgtcactc acgccagtct ggaagggcgc gtgaaacgtg tgacggataa
    29881 accgctgata ttgattaata actatattgg cgatccaaaa ctcgacactt tatttaatca
    29941 attaaccgcc gaacataaac actgattaca gaggtaaaaa tgaaaaccaa agttgctgct
    30001 atttatggca agcgggatgt ccgtctgcgc gaatttgaac tgccagaaat taccgataat
    30061 gaactgttag tgagtgtaat ttctgacagc gtctgtttat cgacctggaa agcggcgtta
    30121 ctcggtagtg aacataaacg cgtacccgac gatttagaaa atcatccggt cattaccggg
    30181 catgaatgtg ccggggttat tgtcgaagtg ggtaaaaatc tcactggcaa atataaaaaa
    30241 ggccagcgtt ttgtattgca accggcgatg gggttaccaa gcggatattc agcaggctac
    30301 agctacgaat attttggcgg taacgccacc tatatgatta ttcccgaaat cgccattaat
    30361 ttgggctgcg tattaccgta tcacggctct tattttgctg cggcgtcgct ggtagagcct
    30421 atgtgctgca ttattggtgc ttatcatgcc aattatcaca ccacgcaata tgtttatgag
    30481 catcgcatgg gcgtcaaacc tggcggcaat attgcactgc tggcgtgtgc gggtccgatg
    30541 ggcattggcg ctatcgatta cgccattaac ggtggcatac aaccgtcacg ggtggtggtg
    30601 gtcgatatcg acgacaaacg cctggcgcag gtgcagaagc tgctgccggt ggatctggcg
    30661 gccagtaaag gcattgagct ggtgtatgtg aataccaaag ggatgagcga tccggtccag
    30721 acgctgcggg cgctgacagg agatgtcggg ttcgatgaca tttttgttta tgcggcggtg
    30781 cctgctgtcg ttgagatggc tgacgaatta ctggcggaag atggctgtct gaacttcttt
    30841 gccgggccga cggataaaaa cttcaaagtg ccgtttaatt tctacaacgt ccattacaac
    30901 agcacgcacg tagtcggtac atccggcggt tcaacggacg acatgaaaga ggcgattgcg
    30961 ttaagcgcca cggggcagtt gcagccgtcc tttatggtca cgcatatcgg tgggctggat
    31021 gcggtgccag ataccgtgct caatctgccg gatatccctg gcggtaaaaa actcatttat
    31081 aacggcgtga ccatgccgct cactgccatt gccgattttg ccgaaaaagg caaaaccgat
    31141 ccgctattta aagagttggc gcggctggtt gaggaaacgc acggcatctg gaatgaacag
    31201 gccgagaaat atctgctggc acaatttggc attgatatcg gggaggccgc gcaatgatgt
    31261 ccctggcgtg gccattattt cgcgttacgg aacaggcagc gttggctgcc tggccgcaaa
    31321 ccggatgtgg cgacaaaaat aaaattgatg gcctggcggt caccgcgatg cgccaggcat
    31381 taaacgacgt cgcttttcgt ggacgagtgg ttatcggcga aggagaaatt gaccatgcgc
    31441 cgatgctgtg gattggcgaa gaggtgggca aaggcgatgg gccagaagtc gatatcgcgg
    31501 ttgatcccat tgaaggcacg cggatggtgg cgatggggca gagcaatgcg ctggcggtca
    31561 tggctttcgc ccctcgggat agcctgttac atgcgcccga tatgtatatg aaaaagctgg
    31621 ttgttaatcg actggctgcc ggggtgattg atctgtcttt gcctctggcg gacaacttgc
    31681 gtaacgtggc gcgggcgtta ggtaaaccgc tggacaagct gcgcatggtt acgctggata
    31741 aaccgcgcct gagtgccgcg attgaggaag caacacagct gggcgtgaag gtttttgccc
    31801 tgccggatgg cgatgtcgcc gccagcgtgc tgacctgctg gcaggataat ccttacgatg
    31861 tgatgtacac catcggcggt gcgccagaag gcgtgatttc tgcctgtgcc gttaaagcat
    31921 taggcgggga tatgcaggcc gagctgattg acttttgtca ggctaaaggg gattacacgg
    31981 aaaatcggca gattgcagag caggagagca agcgttgcaa ggcaatgggc gttgacgtta
    32041 accgtgtgta ctcgctcgat gaactggtga gggggaacga tatcctcttt agcgctacgg
    32101 gcgtgacggg cggcgagttg gtgaacggta tccaacagac ggcgaatggg gtgcggacgc
    32161 agacattact gatcggcggc gcggaccaaa cgtgtaatat aatagactcc ctgcattgat
    32221 ggtgatctat cggaagacca atggcgaagc tgacagaaga tgatgtgctt gagcaactgg
    32281 atgcacagga caatttattt tcatttatga aaactgcgca ttctattttg ctccagggga
    32341 tacgccagtt tctgccgtcg ctgtttgtcg ataacgatga agagatcgtg gaatatgcag
    32401 tgaagccgtt actcgcccaa agcggcccgc ttgacgatat tgatgttgcg ctgcgtttga
    32461 tttatgcgct ggggaaaatg gataaatggc tgtacgccga tatcacgcat ttttcccagt
    32521 tctggcatta cctgaacgaa caggatgaaa cgcccggatt tgccgatgac atgacctggg
    32581 attttatcag caacgtcaat agcataaccc gcaatgcgat gctctatgac gcgttgaaag
    32641 cgatgaagtt cgccgatttc gccgtctggt cagaggcgcg ttttagcggg atggtcaaaa
    32701 cggcgctgac gctggcagta acgacaatct taaaggaatt aacgccgtga aaattgaatt
    32761 aacggtgaat gggctgaaga tacaggcaca gtaccagaac gaagagattg aaaatgtaca
    32821 taaaccgttg ctgcgtatgc tggcggcctt acagacggta aatccgcagc ggcgtacggt
    32881 ggtttttctt tgcgctccgc cggggacggg taaatctacc ctaaccacct tctgggaata
    32941 tctcgcgcaa caagacccgg agctgcccgc tattcagacg ctcccgatgg atggttttca
    33001 ccattacaat agctggctgg acgcgcatca attgcgcccc ttcaaaggcg caccagagac
    33061 atttgacgtt gcgaaactgg cggaaaatct gcgccaggtg gtggaagggg attgtacgtg
    33121 gccgcagtac gatcgacaaa agcatgatcc tgttgaagat gcgttgcacg ttaccgcacc
    33181 actcgtcatc gtcgaaggaa actggttgtt gctggacgac gagaagtggt gccagcttgc
    33241 gcaattttgt gatttctcga tctttatcaa cgcgccagct actgcgttgc gggagcgtct
    33301 ggtggggcgc aagctggcgg gaggggtatc gctggcagat gcagaagcct tttatgaccg
    33361 taccgacggg ccgaatgttc gccgggtgct ggaagagagt ctgcctgcaa atttgacctt
    33421 aatgatgacc gctacgggtg aatatcgtct ggtggattaa cgttcctgat tacaccatct
    33481 tccagtcgca ccacacgaga gaaatgacgc cgggtaaatg cggcgtcatg actgattgct
    33541 accacgctcg tgcctcgctg gcggcacttc tccagccagc tttcaaacac gctcaaccaa
    33601 ttttcgtcaa aatcccgact cggctcgtcc agcagtaata gtggcggaga gagtgcttcc
    33661 aggcaggcga cggcgaccat ccgtcgctgc gcactatgca gatccaacgg gtgcgagttg
    33721 gcagtgtctg ctaactgaca acattgcagg gcagcatggg tacgttgagt gatttcatcc
    33781 gccgggcatt tttgcagttt caaaccaaat gcgatctcgt cagcgacggt gctgtgaaaa
    33841 agttggtttt ccgcttcctg aaacagtacg ccaacctttg ccgcgcgctg gcggtttttc
    33901 aggtttttca tcgcctgttg ttgcagcata accgtaccgg ctgtgggcgt aagaagacct
    33961 gccattactc gtaacagcgt cgatttccct gcgccattgt caccggtcag cgccaaccat
    34021 tcaccttgtt tgagttgcag agaaatgtcg caaagacaat ccgttgccgc gccaggccag
    34081 cgataagata tctgatttaa cgttaacata gtggctggag cgctccgttt tgcaattgcc
    34141 acgcttgctg gcagtagttc aggaaaggtg tgcgatgacg ttcaaaaaga ataatcagcg
    34201 aaccacgttc aaacgcccag tgttgtaagc gctgcaacag catttcgctg gcctgcggcg
    34261 tcaggcggct aaaggcttca tcaagaatta agagctttgg ctgcatcgcg atggcgcagg
    34321 cgatgaccac gcgctgtgtc tctccaccgg agagtgttgc cgggtgacga tggcgcaacg
    34381 gctggcactc ggtcagagtc agggcggcat caatccgcgc cataatctct gcttcagcaa
    34441 gacacagatt ttctggccca aaggcaactt cttcttccac gctaaaggta cagccggaga
    34501 gctgcaaata gggcgattgc tggacgagct ggatggtggc ggattgttca ttaagcggga
    34561 gatgcccaat gggcgtaccg aggagcgtcc ccgtgcctgt tatctctcct ggcagaaagt
    34621 ccggatacca gcccgccatc agctgtgcca gcgtactttt gccactgccg ttgtcaccaa
    34681 agatggcgac catccccggt ttgacatagt gaaagtcata acagaacgga ggatgcgttg
    34741 agtgtgtcgg gcaataacgg aactgttcta acgtaaccat atccacgctc cgaattctgt
    34801 cagcatcagc aagatcatgg tgtaacgcgc tacgcgttgt agggtgctat cggctggtgc
    34861 ccacaacgtt gttcgtcggt tgtggatccg aaatgcccgc atatccagcg ccgcaccacg
    34921 gacagccaga tcgttaagcg cattgtgtgt tagcggaata attaacgcgg gcatcgcacg
    34981 taaacgctga taccagccct catccagcgg cacgccgcgg gcacgctgcg cttcatgaat
    35041 aatcgctaat tgccgtttta actgctcaac gaccaataac ggcccggcaa acagataagc
    35101 gacgcctggc ggcaggcgag aagcaaacag agcgcgaata aaacattgca cggggacgta
    35161 ctgcatccac aactgtgagg ttgaaacaat caccagaatc cgcaaccaga gcgtaatagc
    35221 atgcgaccac cgttctggcg agcgtggcgt gccgcttagc cattctggta gccagccacc
    35281 gtgtaccagc cacagcccgg ctcccagcga gaacatcagc cagacgacat attttgcccg
    35341 ccgccgcgtg gcctttagcg caataaggca gaaaaaggtc gccgcgctat agatcggcaa
    35401 aatggtctgc gcgggcagga taagcgtggt gcaggccgcc aatgcccaca gtgttaatga
    35461 ggtaaatgga tgcatttagc gcacggcagc cattgccgga aaatggcgcg tggtgcgaat
    35521 cggcagctgg cgcagtaaaa gccagacaat caccgctgta agaattttat cgaccaggtt
    35581 cgcgcctatc accgtaatcg ccaccgactc caccaggttt tgccccatcg agtgcatcca
    35641 ggcaacgaac aaatcggctc cgctaccggt gacaccgccg aacagcgcag tacgcagcgg
    35701 tacagcgacg accgttaccg caagcgtaat aatcacgccg ctgacaacga cttttggcag
    35761 ggtgcgaaac caacccgctc gcgccagcca accggcgacc aggccgatga ccatcgcgac
    35821 gggtgcaaat gcggcggcga tcgggtcggt cagcaatccc cagagtaaat tggtcagtaa
    35881 gccggtcagc attccgatca ccggaccaag cagtaccgcg ctaataagtg tgccgatgga
    35941 gtcgagaaat ataggtaatt tgaccatgct ggccagttgc ccgccgatca tattgatggc
    36001 gatagatata acgatcaaca ccagtgcctg actggaaaag tggctacgcg ccataagaaa
    36061 acctctttga aagtagttat ttaacggggg aaatatgcaa ctcgtcgtaa ccagaaagcg
    36121 cgcaaggttg gcggctaaac gccagttcct caaggctacc gatcaccagt tcaagcgtgg
    36181 tccgcacggt acagccgggc atcatatgac cgccaagcat cgtgccgtgt ggatcggaaa
    36241 cgcagagatg aagatgctca ccactctgtt ccagcgtgcc gtttagtgag atcacttcga
    36301 atgtaccgtt caggagcgtg gtgccttctt gcctggcata gcgcaaggcg acgtcagtca
    36361 ggctaccggt acagcctgca atccaggcgg catgaagttg atgctgctgt gcaaaagcgt
    36421 gtagctggga aaaaacttcc tgcccgggga ggaggcgcag tgcgtaaaag cgtgcggagg
    36481 aagaatatgg gtagggcaat gtcatcattg gagtactccg aaactcggac gtattgcgga
    36541 taatatcgac gctgcatcag tcatattctg atataagtca gggtttgtga cggcacaacg
    36601 aaaagatttt gctacgctta ttcgtgattc tgcaaagtgg aataaattct acaggggcgg
    36661 ggctgaaaac gtgacgtaag tcagataatt gctttctttg actggacaat cattcctttt
    36721 attccacgtt tcgcttatcc tagctgaagc gtttcagtcg attaaatgtt cgacaattaa
    36781 ccaatcagtc gcagtttgcg acaggtaagg tttccccgga cgatttgctg gattactctg
    36841 tctggcaaat ttgctcaggg aaaaccttgc aggagatcta tgaccgtacg cgtagcgata
    36901 aatggcttcg gtcgcatcgg gcgtaatgtg gttcgtgctt tgtatgaatc cggacgccgg
    36961 gcggaaatta ccgtggtggc aatcaacgaa ctggcggatg ctgcgggcat ggcgcatttg
    37021 ttgaaatatg acaccagcca tggccgtttt gcatgggaag tacgacagga acgcgatcaa
    37081 ctttttgttg gtgatgacgc catccgcgta ttgcatgaac gttcactgca atcgctcccc
    37141 tggcgtgaac ttggcgttga tgtagtcctc gactgcaccg gcgtatatgg ctcccgcgag
    37201 catggcgaag cgcatattgc cgccggggcc aaaaaagtgc tcttttcaca tcctggcagt
    37261 aacgatctcg acgcgaccgt tgtttacggc gtcaatcagg atcaacttcg tgcggaacac
    37321 cgcatcgttt ctaacgcttc ctgtaccacg aattgcataa ttcccgtcat caaattgtta
    37381 gatgatgcgt acggtattga gtccggcact gtgaccacaa ttcactccgc catgcacgat
    37441 caacaggtta ttgatgcata ccatcctgac ctgcgtcgca cccgggcagc cagccagtcg
    37501 atcattccgg tcgatactaa actggccgcc ggtatcacac gattttttcc gcaatttaac
    37561 gatcgctttg aagcgattgc ggtacgtgtg ccaaccataa atgtgacggc aatcgattta
    37621 agcgtgacgg tgaagaaacc tgtaaaagcc aatgaagtca acctgttgct gcaaaaagca
    37681 gcacaaggtg catttcatgg tatagttgac tatacggaat tgccgttggt ctctgtagat
    37741 tttaaccacg atccgcacag tgccattgtc gatggcaccc aaacccgggt cagtggcgca
    37801 cacctgatca aaacgttggt ctggtgcgat aacgaatggg gctttgctaa ccgaatgctc
    37861 gacacgacgt tagctatggc tactgttgct ttcaggtaag acgcaagcag cgtctgcaaa
    37921 acttttagaa tcaacgagag gattcaccat gtctgtaatt aagatgaccg atctggatct
    37981 tgctgggaaa cgtgtattta tccgtgcgga tctgaacgta ccagtaaaag acgggaaagt
    38041 aaccagcgac gcgcgtatcc gtgcttctct gccgaccatc gaactggccc tgaaacaagg
    38101 cgcaaaagtg atggtaactt cccacctggg tcgtcctacc gaaggcgagt acaacgaaga
    38161 attctctctg ctgccggttg ttaactacct gaaagacaaa ctgtctaacc cggttcgtct
    38221 ggttaaagat tacctcgacg gcgttgacgt tgctgaaggt gaactggttg ttctggaaaa
    38281 cgttcgcttc aacaaaggcg agaagaaaga cgacgaaacc ctgtccaaaa aatacgctgc
    38341 actgtgtgac gtgttcgtaa tggacgcatt cggtactgct caccgcgcgc aggcttctac
    38401 tcacggtatc ggtaaattcg ctgacgttgc gtgcgcaggc ccgctgctgg cagctgaact
    38461 ggacgcgctg ggtaaagcac tgaaagaacc tgctcgcccg atggtggcta tcgttggtgg
    38521 ttctaaagta tctaccaaac tgaccgtttt ggactccctg tctaaaatcg ctgaccagct
    38581 gattgttggt ggtggtatcg ctaacacctt tatcgcggca caaggccacg atgtgggtaa
    38641 atccctgtac gaagctgacc tggttgacga agctaaacgt ctgctgacca cctgcaacat
    38701 cccggttccg tctgatgttc gcgtagcaac cgagttctct gaaactgcac cggctaccct
    38761 gaaatctgtt aacgatgtga aagctgacga gcagatcctg gatatcggtg atgcttccgc
    38821 tcaggaactg gctgaaatcc tgaagaatgc gaaaaccatt ctgtggaacg gtccggttgg
    38881 cgtgttcgaa ttcccgaact tccgcaaagg tactgaaatc gtggctaacg ctatcgcaga
    38941 cagcgaagcg ttctccatcg ctggcggcgg cgacactctg gcagcaatcg acctgttcgg
    39001 cattgctgac aaaatctcct acatctccac tggcggcggc gcattcctcg aattcgtgga
    39061 aggtaaagta ctgcctgcag tagcgatgct cgaagagcgc gctaagaagt aaaaaatcac
    39121 agggcaggga aacctgccct tgtttcagcg cgcttttaga gcacgcattt tttcatactc
    39181 taaataattc gagttgcagg aaggcgacaa gagagtgaat ccccaggagc ttacataagt
    39241 aagtgactgg ggtgagcgaa cgcagacgca gcacatgcaa cttgaagtat gatgagtata
    39301 aggcccgacg atacaggaca agagacatgt ctaagatttt tgatttcgta aaacctggcg
    39361 taatcactgg tgatgacgta cagaaagttt tccaggtagc aaaagaaaac aacttcgcac
    39421 tgccagcagt aaactgcgtc ggtactgact ccatcaacgc cgtactggaa actgctgcta
    39481 aagttaaagc gccggttatc gttcagttct ccaacggtgg tgcttccttt atcgctggta
    39541 aaggcgtgaa atctgacgtt ccgcagggtg ctgctatcct gggcgcgatc tctggtgcgc
    39601 atcacgttca ccagatggct gaacattatg gtgttccggt tatcctgcac actgaccact
    39661 gcgcgaagaa actgctgccg tggatcgacg gtctgctgga cgcgggtgaa aaacacttcg
    39721 cagctaccgg taagccgctg ttctcttctc acatgatcga cctgtctgaa gaatctctgc
    39781 aagagaacat cgaaatctgc tctaaatacc tggagcgtat gtccaaaatc ggcatgactc
    39841 tggaaatcga actgggttgc accggtggtg aagaagacgg cgtggacaac agccacatgg
    39901 acgcttctgc actgtacacc cagccggaag acgttgatta cgcatacacc gagctgagca
    39961 aaatcagccc gcgtttcacc atcgctgcgt ccttcggtaa cgtacacggt gtttacaagc
    40021 cgggtaacgt ggttctgact ccgaccatcc tgcgtgattc tcaggaatat gtttctaaga
    40081 aacacaacct gccgcacaac agcctgagct tcgtattcca cggtggttcc ggttctactg
    40141 ctcaggaaat caaagactcc gtaagctacg gcgtagtgaa aatgaacatc gataccgata
    40201 cccaatgggc aacctgggaa ggcgttctga actactacaa agcgaacgaa gcttatctgc
    40261 agggtcagct gggtaacccg aaaggcgaag atcagccgaa caagaaatac tacgatccgc
    40321 gcgtatggct gcgtgccggt cagacttcga tgatcgctcg tctggaaaaa gcattccagg
    40381 aactgaacgc gatcgacgtt ctgtaagata ttcctttctg cttatctcaa ggcccgctct
    40441 gcgggtcttt ttttcgccaa aagcaaaatt gcctgatgcg ctacgcttat caggcctaca
    40501 cgtatcctgc aatatattga atttgcaggt ttttgtaggc cggataaggc gttcacgccg
    40561 catccggcat gacaaagcgc acattgatat ctgtctgagc cccgctctgc ggggattttt
    40621 tcgccaaaag caaggtgatt cagatgagaa atctgtgacc tatttggcaa aattatgctt
    40681 tattgtttac ccttgtcaga ctgcccgtca taaggcggcg gagtgtattt ctccattttg
    40741 agtcagttga aaaggaatat tgaatggaag atttgaatgt tgtcgatagc ataaacggcg
    40801 cgggaagctg gctggtagct aaccaggcgc tgctgctaag ttatgcagta aacatcgtgg
    40861 cggcactcgc gatcatcatc gttggtttga ttatcgcgcg gatgatttcc aacgcggtga
    40921 atcgcctgat gatctcccgt aaaatcgatg ccactgttgc tgattttctt tctgcattag
    40981 tccgttacgg tattatcgcc tttacgctaa tcgctgccct gggacgcgtg ggtgtacaaa
    41041 cagcgtcagt cattgctgta ctcggtgccg caggcttagc tgttggtctg gctttgcagg
    41101 ggtcactttc taacctggcc gctggcgtgt tacttgtcat gttccgaccg ttccgtgccg
    41161 gagaatatgt tgacctcggc ggcgtagccg gtactgtgct gagtgtgcag attttctcca
    41221 ccaccatgcg tactgcagac ggtaaaatta tcgttattcc gaacggtaaa attattgccg
    41281 gaaatattat taacttctcc cgcgagccag ttcgccgtaa cgaatttatt attggcgtgg
    41341 cgtatgattc cgatatcgat caggttaagc agatcctgac caatattatc cagtctgaag
    41401 atcgcatttt gaaagatcgc gaaatgactg tgcgcctgaa cgaacttggt gcatcgtcga
    41461 ttaatttcgt ggtccgcgtc tggagcaaca gcggcgatct gcaaaacgtg tactgggatg
    41521 tgctggagcg tattaaacgt gaatttgatg ccgccggtat cagcttcccg tacccgcaaa
    41581 tggatgtgaa ctttaagcgg gtgaaagaag acaaagctgc gtaatcaacg ctgaggccag
    41641 ataatactct ctggcctctc tcttattagt tattctgatt gccaattaat attatcaatt
    41701 tccgctaata acaatcccgc gatatagtct ctgcatcaga tacttaattc ggaatatcca
    41761 acgtgttttc ttattacttt caaggtcttg atcttggggc agctatgatt ctgccgctcg
    41821 gcccacaaaa tgcttttgtg atgaatcagg gcattcgtcg tcagtaccac attatgattg
    41881 ccttactttg cgctatcagc gatttggtcc tgatttgcgc ggggattttt ggtggtagcg
    41941 cgttattgat gcagtcgccg tggttgctgg cgctggtcac ctggggcggc gtagtcttct
    42001 tgctctggta tggttttggc gcttttaaaa cagcgatgag tagtaatatt gagttagcta
    42061 gtgccgaagt cctgaagcaa ggcagatgga aaattatcgc caccatgttg gcagtgacct
    42121 ggctgaatcc gcatgtttac ctggatactt ttgttgtact gggcagcctt ggcgggcagc
    42181 ttgacgtcga accaaacgct cgggacaatt agcgcctctt tcctgtggtt ctttggtctg
    42241 gctattctcg cagcctggct ggcaccgcgc ctgcgcacgg caaaatcaca gcgcattatc
    42301 aatctggtag tgggatgtgt catgtggttt atagccttgc agctggcgag agacggtatt
    42361 gctcatgcac aagccttgtt cagttaggcg ctatctgatg gaaaaataaa acagaggcgc
    42421 taagcttgcc tccagaggtc ctgaattttg ggcaagtcaa ttaacacgga gagactaacg
    42481 tgaagttcaa agttatcgcc ctagcggcat taatgggtat tagcgggatg gcagcgcagg
    42541 ctaacgaatt gccggatgga ccgcatattg tcacctccgg tacggcaagt gtggatgcgg
    42601 tgccagacat tgccactctt gcgattgaag ttaacgtggc cgcgaaggat gccgctacag
    42661 ccaagaaaca ggcagatgag cgcgtcgcac aatacatttc cttccttgaa ctcaatcaga
    42721 tcgcgaaaaa agatatcagc tcagcgaatt tacgcaccca gccagattat gattatcagg
    42781 atggtaaaag tatcctgaaa ggctaccgcg ctgtgagaac ggtggaagtc acgctccgtc
    42841 agttagacaa actgaattcc ttgctggatg gcgcgctgaa ggcgggtctt aacgaaattc
    42901 gttcagtgtc gctgggcgtg gcgcagccgg atgcctataa agacaaagcg cgtaaggcag
    42961 cgattgataa cgcgattcat caggcgcagg aactggcgaa cggctttcat cgtaaactgg
    43021 ggccggtata tagcgtgcgc taccatgttt ccaactatca gcccagccca atggtgcgga
    43081 tgatgaaagc cgatgccgcg ccggtgtctg cccaggaaac ttacgagcag gccgctattc
    43141 agtttgatga tcaggtcgat gtggtcttcc agttagaacc tgtggatcaa caacccgcta
    43201 aaacacctgc agcacaataa tacttgtagc ccgacgtatt cgtcgggcta atgcctttac
    43261 ccgatattgc ctcctttttc tgtaattttc tgtaaaacca tgacgtaatc acctatcttt
    43321 gctcgcggtt tcatcaagga taggctgact gaaacggtta agcggacaag gatgcgggg
//
LOCUS       DAZPEB010000009        43052 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZPEB010000009 DAZPEB010000000
VERSION     DAZPEB010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43052
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.009"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(137..805)
                     /gene="artM"
                     /locus_tag="ACHURJ_000486"
     CDS             complement(137..805)
                     /gene="artM"
                     /locus_tag="ACHURJ_000486"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFR1279561.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(805..1521)
                     /gene="artQ"
                     /locus_tag="ACHURJ_000487"
     CDS             complement(805..1521)
                     /gene="artQ"
                     /locus_tag="ACHURJ_000487"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFR1279562.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESEKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(1528..2259)
                     /gene="artI"
                     /locus_tag="ACHURJ_000488"
     CDS             complement(1528..2259)
                     /gene="artI"
                     /locus_tag="ACHURJ_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFR1279563.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(2277..3005)
                     /gene="artP"
                     /locus_tag="ACHURJ_000489"
     CDS             complement(2277..3005)
                     /gene="artP"
                     /locus_tag="ACHURJ_000489"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFR1279564.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(3223..3738)
                     /locus_tag="ACHURJ_000490"
     CDS             complement(3223..3738)
                     /locus_tag="ACHURJ_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR1279565.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGTRSGPCVEGGPGNV
                     AQQFYDYRILHRSNDITALRPYLSDKLATLLSDASRDNNHRELLTNDPFSSRTTLPES
                     ARVASASTIPNRDARNIPLRVDLKQGDQSWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            3864..4187
                     /locus_tag="ACHURJ_000491"
     CDS             3864..4187
                     /locus_tag="ACHURJ_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFR1279566.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            4184..5014
                     /gene="amiD"
                     /locus_tag="ACHURJ_000492"
     CDS             4184..5014
                     /gene="amiD"
                     /locus_tag="ACHURJ_000492"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFR1279567.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(5011..6021)
                     /locus_tag="ACHURJ_000493"
     CDS             complement(5011..6021)
                     /locus_tag="ACHURJ_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFR1279568.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMFSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLCSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     FRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGKL
                     PR"
     gene            complement(6120..7550)
                     /locus_tag="ACHURJ_000494"
     CDS             complement(6120..7550)
                     /locus_tag="ACHURJ_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFR1279569.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(7561..8562)
                     /gene="ltaE"
                     /locus_tag="ACHURJ_000495"
     CDS             complement(7561..8562)
                     /gene="ltaE"
                     /locus_tag="ACHURJ_000495"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFR1279570.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKVRNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(8599..10317)
                     /gene="poxB"
                     /locus_tag="ACHURJ_000496"
     CDS             complement(8599..10317)
                     /gene="poxB"
                     /locus_tag="ACHURJ_000496"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFR1279571.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(10450..11418)
                     /gene="hcr"
                     /locus_tag="ACHURJ_000497"
     CDS             complement(10450..11418)
                     /gene="hcr"
                     /locus_tag="ACHURJ_000497"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFR1279572.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVHTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAKAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(11430..13082)
                     /gene="hcp"
                     /locus_tag="ACHURJ_000498"
                     /gene_synonym="priS"
     CDS             complement(11430..13082)
                     /gene="hcp"
                     /locus_tag="ACHURJ_000498"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFR1279573.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVSAYDDRIWTRS
                     IVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATIVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(13226..14125)
                     /gene="lysO"
                     /locus_tag="ACHURJ_000499"
     CDS             complement(13226..14125)
                     /gene="lysO"
                     /locus_tag="ACHURJ_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFR1279574.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(14619..15314)
                     /gene="aqpZ"
                     /locus_tag="ACHURJ_000500"
     CDS             complement(14619..15314)
                     /gene="aqpZ"
                     /locus_tag="ACHURJ_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFR1279575.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATAKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            15740..17398
                     /locus_tag="ACHURJ_000501"
     CDS             15740..17398
                     /locus_tag="ACHURJ_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFR1279576.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAIEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(17395..18351)
                     /locus_tag="ACHURJ_000502"
     CDS             complement(17395..18351)
                     /locus_tag="ACHURJ_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFR1279577.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLKLTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            18502..19617
                     /gene="macA"
                     /locus_tag="ACHURJ_000503"
     CDS             18502..19617
                     /gene="macA"
                     /locus_tag="ACHURJ_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFR1279578.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAEIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            19614..21560
                     /gene="macB"
                     /locus_tag="ACHURJ_000504"
     CDS             19614..21560
                     /gene="macB"
                     /locus_tag="ACHURJ_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFR1279579.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHMVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALVRE"
     gene            complement(21633..21857)
                     /gene="cspD"
                     /locus_tag="ACHURJ_000505"
     CDS             complement(21633..21857)
                     /gene="cspD"
                     /locus_tag="ACHURJ_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFR1279580.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            22007..22081
                     /gene="yljB"
                     /locus_tag="ACHURJ_000506"
     CDS             22007..22081
                     /gene="yljB"
                     /locus_tag="ACHURJ_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFR1279581.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            22180..22500
                     /gene="clpS"
                     /locus_tag="ACHURJ_000507"
     CDS             22180..22500
                     /gene="clpS"
                     /locus_tag="ACHURJ_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFR1279582.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            22531..24807
                     /gene="clpA"
                     /locus_tag="ACHURJ_000508"
     CDS             22531..24807
                     /gene="clpA"
                     /locus_tag="ACHURJ_000508"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFR1279583.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(25151..25238)
                     /locus_tag="ACHURJ_000509"
     tRNA            complement(25151..25238)
                     /locus_tag="ACHURJ_000509"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25202..25204),aa:Ser,seq:gga)
     gene            complement(25492..25710)
                     /gene="infA"
                     /locus_tag="ACHURJ_000510"
     CDS             complement(25492..25710)
                     /gene="infA"
                     /locus_tag="ACHURJ_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFR1279584.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(25995..26699)
                     /gene="aat"
                     /locus_tag="ACHURJ_000511"
     CDS             complement(25995..26699)
                     /gene="aat"
                     /locus_tag="ACHURJ_000511"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFR1279585.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(26741..28462)
                     /gene="cydC"
                     /locus_tag="ACHURJ_000512"
     CDS             complement(26741..28462)
                     /gene="cydC"
                     /locus_tag="ACHURJ_000512"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFR1279586.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMMVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAESL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(28463..30229)
                     /gene="cydD"
                     /locus_tag="ACHURJ_000513"
     CDS             complement(28463..30229)
                     /gene="cydD"
                     /locus_tag="ACHURJ_000513"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFR1279587.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQCGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEHELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(30352..31317)
                     /gene="trxB"
                     /locus_tag="ACHURJ_000514"
     CDS             complement(30352..31317)
                     /gene="trxB"
                     /locus_tag="ACHURJ_000514"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFR1279588.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            31862..32356
                     /gene="lrp"
                     /locus_tag="ACHURJ_000515"
     CDS             31862..32356
                     /gene="lrp"
                     /locus_tag="ACHURJ_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFR1279589.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            32491..36519
                     /locus_tag="ACHURJ_000516"
     CDS             32491..36519
                     /locus_tag="ACHURJ_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFR1279590.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDEE
                     EIIYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVTSVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYATAPEQPAQQPYYAPA
                     PEQPVAGNAWQAEEQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSAAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREARRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQLVAPQQQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQQVAPQPQYQQPQQPVAPQQQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLAPPPFD"
     gene            36674..37285
                     /gene="lolA"
                     /locus_tag="ACHURJ_000517"
     CDS             36674..37285
                     /gene="lolA"
                     /locus_tag="ACHURJ_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFR1279591.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            37296..38639
                     /gene="rarA"
                     /locus_tag="ACHURJ_000518"
                     /gene_synonym="mgsA"
     CDS             37296..38639
                     /gene="rarA"
                     /locus_tag="ACHURJ_000518"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFR1279592.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPREIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            38730..40022
                     /gene="serS"
                     /locus_tag="ACHURJ_000519"
     CDS             38730..40022
                     /gene="serS"
                     /locus_tag="ACHURJ_000519"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFR1279593.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            40261..42705
                     /gene="dmsA"
                     /locus_tag="ACHURJ_000520"
                     /pseudo
     CDS             40261..42705
                     /gene="dmsA"
                     /locus_tag="ACHURJ_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            42716..>43052
                     /gene="dmsB"
                     /locus_tag="ACHURJ_000521"
     CDS             42716..>43052
                     /gene="dmsB"
                     /locus_tag="ACHURJ_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFR1279594.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYG"
BASE COUNT        10703 a        10943 c        11487 g         9919 t
ORIGIN      
        1 tataagaatt tatgcactat gtaggccgga taaggcgtcc acgccgcatc cggcacaggc
       61 accgtgctga tgtctgatgc gacgctggcg cgtcttatca gacctacaaa ccccccggcg
      121 aatgtacgca gctacattaa ttccgccgtt cgaatgccag tgctttgcgc tcgatcagac
      181 gcatcatcag cgtcagcagg ccgttgacga ccaggtaaat aatccctgcc gcaccgaaca
      241 ccattacatc gtaggtgcgt ccgtacaaca actggctgta tcccatcact tccatcagcg
      301 taatggtgta agccagagag gtacttttga acaccagcac cacttcgttg gaataagaag
      361 agagcgagcg tttaaaggca tacggcagca ggatcgccag cgtatctttt ttgctcattc
      421 ccagggcgct acaggactgc cactgacctt ctgggatcgc acgaattgca ccgtaaaaca
      481 gctgcgtggt atacgccgca ctgttcagcg ataacgcaat cagcgcacat aaccacggtt
      541 ctgacaacaa atgccacagt gccggatact cctgcaaagt cggaaactgg cccggcccgt
      601 aataaatcag gaagatctgc accagcagcg gcgtaccggt aaacagcgtg atataacccc
      661 gcaccagcca caccagcacc ggcgttttca gcgtcaggat gatggtaaaa atcaatgcca
      721 gaatcagtgc cacaatcagc gaggcaacgg ttagcgtcag gctggtgtgt agccctttca
      781 tcagttcggg taaatactca aacattagct gggcctccgc tcaaaacgtg tcgcgcgcag
      841 gtcaatgcgt ttgagaatgt actgactgag cagggtgatc accaggtaaa tcgccgccgc
      901 cacaatatac caggtaaatg gttcctgggt acgagtagcg atgctttttg tttgcagcat
      961 taaatcattc acactaatca aactgaccag cgcggtatct ttcagcagca ccagccactg
     1021 gttaccgagg ccaggcagcg catgacgcca catctgcggc atcaccagac ggaaaaagat
     1081 agccgatttc gacagcccca gcgcctgacc agactcccac tgacccaccg gcaccgcttt
     1141 caacgcgccc cgaagcgttt gcgaggcata ggcggcatac agcagtgaca gagcgatgac
     1201 accacaaagg aacgggctaa cgtcgaagtt ctcaatgtcc atctgcactg ggatctgcac
     1261 gaacccaaga ttgatagtga agccatccga aagcgtcagc agcagctgcg aggagccaaa
     1321 atagataaac agcaccacca gaatttctgg caggccacgc agaatggtta ccagcgctga
     1381 acctgcccac gcgacaggac gccatttttc cgactcccat accgcaaaga acatcgccag
     1441 cgccagcccg acaatcaatg cacaaacggc aaggccgacg gtcatcccgg cggcgcttgc
     1501 taaaggaaaa aattcattca tcaggaatta cttctggaac catttgttgt agatggtttc
     1561 gtaagtgcca tctttcttca ctttttccag cgcagtgttg agtttctgct gcagctcagt
     1621 gttgccctga cgtaccgcga tgccgaggcc agtgccgaag taatctttat cggtcacttt
     1681 gtcgcccacc gccgccagtt tcgggttatc tttcagccac tcagtgacca ctgcggtgtc
     1741 accgaagacg ccgtcgatac gcccgttttg cagatccagt tttgcgttct ggtagctgtc
     1801 atacggaacg gtagtgattt ccgggtgctt atccataatg aatttctggt gtgtcgtccc
     1861 gttctgtacg ccgacttttt tgcctttcag ctgatcaaca ctggtgtatt tgccttgctg
     1921 acccacaaac agggcagagt tgtcatagta cggggtggta aacagcacct gcttttcacg
     1981 ctccggagtg atatccatgc ccgccatcac ggcttctacg cgacggaatt tcaggcttgg
     2041 gatcaggctg tcaaacgcct ggttagagaa agtgcaggtt gcatcaatct ctttacacag
     2101 cgcttgtgcc aggtcgacgt caaaaccaac gatctggttg tttgcatcaa tcgattcaaa
     2161 cggaggatag gatgcttcgg tagcaaaacg aatggtttcg gcagctgtgg cggaaagact
     2221 aaaacctgca attaacgcgg caatcagaac ttttttcatt gttgttatcc cgaatcttag
     2281 tgagagagat agtttttaaa tgcttcggtt tgcggctcgg taaagcagct cgcgtcgcct
     2341 tgttctacga tatgaccatt ttccatatac accactcggc tggcggtttt acgcgccact
     2401 tcaacttcgt gggtgacgat cacctgggta atattcgttt ctgccagctc acgaatgatg
     2461 ctgacgattt gtgccgtaat ttccgggtcc agtgcggcgg tcggttcatc gaacagcagt
     2521 acctgcggtt ccatcatcaa cgcacgggca atagcaacac gctgctgctg accaccagaa
     2581 agatgcagcg ggtaacgatc gctataaggt ttgagacgca gacgttccag cagtttttct
     2641 gcacgggcca gcgcctgatc tttactcaac cccagtacac ggcagggcgc ttcaatcagg
     2701 ttttgctgca cggtcagatg cggccacagg ttgtattgct gaaacaccat gccaacgtta
     2761 cgacgcaaat cgcgaatcgc tttgtcagag ggggttttgg tgaaatcgaa atggttgcct
     2821 gcaatgttga gcgtaccgga gcgcggcatc tcaagcagat tgagtacacg cagcagcgag
     2881 cttttacccg cgccgctggg gccaagtaac accagcgttt cgccctgtgg gcaatccagc
     2941 gtgatatcga acagcgcctg atgcgcgccg tagaagcaat taatgccgtt taattgaata
     3001 ctcattgaca ctcgtatact ggcagtctga tagctattga ggtcgaagat agtacctttg
     3061 acagaataat tatgcaatat ttctgcttta aaagttaaaa gcaaagcgca ttattcaata
     3121 aacatagcac aaaataacgg gggcggtggt cggcgagcat aaatgtcggc attcctcacg
     3181 aaatgccgga caatttacgg ggtttattgg ttgatcaagg cgttagcgat tctcgatgga
     3241 ctgacggagc gtacccgccg tggcatgaac gctgccgcct aagtagcgta catcgtcgat
     3301 cacccaacac tggccttcct gaatcattaa cacttcatcc tgccagctct gatcaccctg
     3361 ttttagatcc acacgcagag gaatatttcg cgcatcacga ttcgggattg ttgaggcact
     3421 ggcaacacgg gcgctctctg gcaaggttgt gcgactggag aatggatcgt tggtcagtag
     3481 ttcccgatgg ttattatccc gggaggcatc gctaagcagt gtcgccagtt tgtcgctcag
     3541 ataagggcgc aaggcggtga tgtcgttgct gcggtgcaaa atgcggtagt cataaaattg
     3601 ctgggccacg ttacccgggc ctccttcaac gcaaggacca ctgcgtgtgc cgttatcttt
     3661 ataagctgga gtgactgtgg tgcaggcact gaggagcagt gcgcagggga taagcattgt
     3721 caatttgctg tagcgcataa tgatttcctt ataagcgatc gctctgaaag cgttctacga
     3781 taataatgat atcctttcaa taatagcgta tcagtctgat aatgcttttg agatcgaagg
     3841 cttagcaaac aaggagatcg atcatgcaat tttctacaac cccaactctg gaaggccaga
     3901 ccatcgttga atattgcggt gtggtgaccg gcgaagcgat tttaggtgcc aatattttcc
     3961 gtgatttctt tgccggtatc cgcgatatcg ttggcggacg ttccggtgcg tatgaaaaag
     4021 aactgcgtaa agcacgggag atcgcctttg aggaattagg ctcccaggcg cgggcgctgg
     4081 gggccgatgc cgtcgtcggt attgatatcg actacgaaac ggtcgggcaa aacggcagta
     4141 tgctgatggt tagcgtcagc ggtacggcgg tgaaaacgcg tcgatgagaa gagtcttctg
     4201 gctggtcgct gccgctctgt tattggcagg gtgtgcaggc gaaaaaggca ttgtcgagaa
     4261 agagggatat cagcttgata cccgacgcca ggcgcaggcg gcgtatccgc gcattaaagt
     4321 gctggtgatc cactacaccg cagatgattt tgatagctcg ctggcgacac tgaccgataa
     4381 gcaggtcagc tcgcattatc tggtccctgc ggtaccaccg cgatacaacg gtaaaccgcg
     4441 catctggcaa ctggtgccgg aacaagaact ggcctggcat gcggggatta gcgcctggcg
     4501 cggggcaacg cgccttaacg acacctctat tggcattgag ctggaaaacc gtggctggca
     4561 aaaatcggcc ggagtgaaat attttgcccc gtttgaaccg gcacagattc aggcgcttat
     4621 tccactggcg aaagatatta ttgcccgtta tcacatcaag ccggaaaacg tagtggcaca
     4681 tgcggatatc gcaccgcagc gcaaagacga tccggggcca ttatttccct ggcagcaact
     4741 ggcgcagcag gggattggtg cctggccgga tgcgcagcgg gttaactttt accttgccgg
     4801 gcgcgcgccg cacacaccgg tagataccgc gtcattgctg gagcttttgg cgcgctacgg
     4861 ttatgacgtt aaacctgata tgacaccgcg cgagcagcgg cgcgtgatta tggcattcca
     4921 gatgcatttc cgcccgacgt tatataacgg cgaagcggat gcagaaactc aggcgattgc
     4981 cgaagcattg ctggagaaat acgggcagga ttagcgcggc agttttccgt ggtcgcgtag
     5041 ccaggcggca gttttctcga taccttcatc cagggtgatg accggctgat aacctaactc
     5101 ttcctgcgca cgcgtaatat ccagcgtaaa gtcaaaattc aacttggaga cgccgtagtg
     5161 ggtcagcggc ggctcttttg ctgacttgcg gaaacgctcc atgctgcggg cgatcatatc
     5221 cagcatcggg taggggacgg aacgaatacg acagtcaata ttcaactcgt cgatcagctt
     5281 ctgcacgatg ctgcacagtg tgcgatgctc gccgttggtg atgttgtaca cacgcccgga
     5341 aggtagctta tcgcaggctt cctggcttgc cagccacatt gcgtgcacgg cattttcata
     5401 gtaggtcata tcgaccagcg cactgccgcc atgcggtaac agaatactgc cgtagtggtg
     5461 catcatatgc gccagacggg gaataaagac tttatcgtgc ggtccgaaca gactttgtgg
     5521 gcgcagaata gtaaagcgcg tttgtggatt cgcctgcgaa aacatattga tcacttcttc
     5581 gctggctgct ttgctgcggg caaactcgtt ggcgaagcgg tgagggcgaa aatcttcttt
     5641 aatatcgcga tggtggtgat aatcgaagta cagggagggg gaagagatat gaataaagtt
     5701 acgcacaccc caggcgacag cccattcacc caggcggcga gtggcgcgaa cgttagccag
     5761 atcgaaagcc tgttgtgtcc cccagggtga ggtaaagctg gagcagtgcc acagcgtatc
     5821 aatgcccgcg agcatcactt tagcttgtga tgaaaccagc tcggtcagat ccgccggaac
     5881 aaactctgcg cccatttttt ccagcaattt gcccattgcc tcgttgcgac cggtcgctcg
     5941 cacgctgatg cctttctggc ataaaaactc taccgcgttt cgacctaagc cgctggtggc
     6001 gccggtaacc agtaccttca tatcaatcca ctgttgttga gaaaataacg tgcgcattct
     6061 tccgtgattt cccccatgat gcaatgggaa acatgaaaga ataacgcagg ttttgtcgat
     6121 taatctgtgc tttgttctgc cagtctggcg atttgttttg ccattccgcg aaaaataaac
     6181 agatgcgcgg ggatcatcaa taaccagtaa aacagccccg gcataccgtg cggatgccag
     6241 aaagcgcgga catcgatagt acgatagtcg cctttatctt ccaggctaaa acacagtcgt
     6301 cccagccccg gcgctttcat gccaaataac aacgtaagtt gtttttccgg ttcaacgaca
     6361 atcactttcc agctatctac cgcatcgcca gtctgtaaat attcgcgctc cgggcggcct
     6421 ttcgccagct tatgaccgat cgcgcggtcc atcaacgccc gtgtctgcca caaaatattg
     6481 ccaaagaaat aacgctcttt accgccgatt tggttcacta cctgccataa agcagcaagg
     6541 ctggcggacg ttttaacggt aaaccccgcc tgtttggcaa aataaccgta ctccggtcgc
     6601 cagcgggcaa aggcctgagc gtcgtagccc cagtcgctgg agttgaccag tttttcctcc
     6661 tctttcaacg tgctacgtac tgcgtcatcg aaagcgatca gccgttgtgg gatgagtgca
     6721 cgtagcgcgg tatcatccgc cagcagatcg tgtttcagcc cctgaatcaa cgccctggcg
     6781 gtggtgggcg gtacggaagt aatcacattg agaaaccaca ccgaaatcca gcgggtgggg
     6841 agggggatgg ggatcaacca gcggcgcttg ccgctcaccg ccataaaatg ttcaaactgt
     6901 tgctgataac tgagcacctc tggtccggcg gcttcgaaga tgcggtgttc gctggccgga
     6961 tgatctaaca acgccaccag atagtgcagc aagttttcca gcgcgatggg cgtggtgcgt
     7021 gaacgtaccc agcgtggcgg cgttaacact ggcaggttgt agaccatatc gcgcatgact
     7081 tcgaacgccg ctgaacctgc gccaacgata attccggctc gaagttcggt cacaggtaca
     7141 ttcgcttcac gaagaatgtc cgccgtagcc tgacgagcac gcagatgatc cgactgctca
     7201 tgtggcgggg cctgcaacga actgagaaag attaattgct taactggtac ttcacgtagc
     7261 gcatcgcgga cgttgagagc cacctggcgc tcctgagcga taaaatcgcc gccttcgccc
     7321 atgctgtgca ccagaaaata gacggtatcg atatcctgca acagggccgg aaggttatcc
     7381 ggccagctga gatcgacttt atggcaactg acgtttgcca gttgcagctt tgcaagcctg
     7441 tcgacatgac gtgccgccgc caggatctga tgcccttgct ggctgagtgt gcgcaccaga
     7501 tgctgaccaa tgtagccact ggcaccgaga actaaaatgc gttgcggcac gtctctctcc
     7561 ttaacgcgcc aggaatgcgc gccagtgggc ggcgacttcc gccagttgtt cgcgcgagac
     7621 gtcaagatgc gtcaccaggc ggacaatcgg cgaggcgtta atcagcacgt ttctcacttt
     7681 catgtattcg cctaacgcgg cagcattttc ttccccgacg cgaacaaaca gcatattagt
     7741 gtcctgacgc atcacatccg cgcctgcttc acgcagttgc tccgccatcc aggcggcgtt
     7801 gtcgtgatct tcctgcaacc gtgcgacgtt atttttcagg gcatacatcc cggcggcagc
     7861 cagaatcccg gactgacgca tcccgccacc ggccattttc cgccagcgga tggcacgttt
     7921 aatgtaatca cgattaccga cgagtaatga accgacaggc gtcccaagac ctttcgacag
     7981 gcaaatggtg aacgaatcac aatattgcgt gatctctttc agttcgcagc cgtaagccac
     8041 cacggcatta aagatgcgcg caccgtcaac atgcagcgcc agattgcgct cgcgggtaaa
     8101 ttcccatgct tctttcaggt attcacgcgg cagcactttg ccgttgtggg tgttttccag
     8161 actgagtaat ttggtgcggg cgaaatggat atcgtcgggt ttgattttca tcgccacttt
     8221 atccagcggt agcgtgccgt cggcagccgc gtcgatgggt tgcggctgaa tactgcccag
     8281 caccgctgcg ccaccggctt caaacagata gttatgcgcg gcctgaccga caatatactc
     8341 ttcgccacgt tcgcagtgac tgagcagagc gaccaggttg gcctgagtgc cggtaggcag
     8401 aaaaatggcg gcttctttac cggaaagctc tgctgcatag tcctgcagag cattaacggt
     8461 agggtcgtct ccgtaaacgt cgtccccaac cggggcggcc atcatcgctt cgagcatggc
     8521 gcggctcggt cgggtaacgg tatcactgcg taaatcaatc atggcatgtc cttattatga
     8581 cgggaaatgc cacccttttt accttagcca gttcgttttc gccagttcga tcacttcatc
     8641 gccgcgtccg ctgatgattg cgcgcaacat atacaagcta aatcctttgg cctgttcgag
     8701 tttgatctgc ggtggaatgg ctaactcttc tttggcgacc accacatcca ccagcaccgg
     8761 accgtcgatg gagaaggcgc gttgcagggc ttcatcaact tcagacgctt tttctacacg
     8821 gatacccgta atgccgcacg cttcggcaat gcgggcaaag tttgtgtcgt gtagttcggt
     8881 gccgtcagtc aaatagccac cagctttcat ctccatcgcc acaaagccca gcacgctgtt
     8941 gttaaagacg acaattttca ctggcagttt catctgcact actgagagga aatcgcccat
     9001 caacatgcta aaaccgccat cgccgcacat ggcgaccacc tgacgttctg gctctgtcgc
     9061 ctgcgcaccc agcgcctgcg gcatggcgtt agccatcgaa ccgtggttaa acgaacctaa
     9121 caggcgacgc ttgccgttca tttttagata acgtgccgcc cacaccgttg gcgtaccaac
     9181 gtcacaggtg aaaatagcgt catcggcggc aaaatgacta atttgctgcg ccagatattg
     9241 cgggtgaatg gctttctcgc tcggtttagc taaatcgtcc agccctttgc gggcgtcgcg
     9301 gtaatcttcc agcgctttat ccagaaactt gcgatcggct ttttcttcca ccaatggaag
     9361 caatgcacgc agagtcgact tgatatcgcc gaccagtgcc atatccacct tgctgtgagc
     9421 gccgatgctg gctgggttga tatcaatctg aatgattttg gcatcggtcg ggtagaaggc
     9481 gcggtaggga aattgcgtgc cgagtagcac taacgtgtcg gcgttcatca tggtatggaa
     9541 acctgacgag aagccgatta acccggtcat tccaacatca tacggattat cgtattcgac
     9601 atgttcttta ccgcgcaggg catgaacaat aggcgcttta attttcccgg caaactcaac
     9661 taactcttta tgcgcccccg cgcagccgct gccacacatc agggcgatat tgctggaata
     9721 acgcagcagt tgcgccagtt tgcgtaactc ttcttcttcc ggcgtcacga ctggttgtgg
     9781 cgcatgatac cagtgcgtgg ttgccccttc tggcgcaggt tttaacgcca cgtcgcctgg
     9841 taacacgaca accgaaacgc cacggttaag caccgctttg cgcatggcaa tcgccagtac
     9901 ttgtgggatc tgctccgggc tggaaaccag ctcgcaatag tgactacatt cgcggaatag
     9961 ctcttgtggg tgggtttcct ggaaatagcc gctgccaatt tcgctggagg gaatatgagc
    10021 ggcaatcgcc agtaccggaa cgtgattgcg gtggcaatca aacaggccgt tgattaagtg
    10081 caggttgccg gggccgcacg atccggcgca gaccgccagt tctccgctaa gttgtgcttc
    10141 agcgccagcg gcaaaggccg ccacttcttc gtggcgggtg gacatccact cgatggtgcc
    10201 catgcgatta agactgtcac taagaccatt cagagagtcg cctgtgactc cccagatgcg
    10261 tttcacccct gccgattcga gtgttttggc gatataagct gcaaccgttt gtttcatggt
    10321 tctccatctc ctgaatgtga taacggtaac aagtttagtt catctgacgg agggggaagg
    10381 gatgggagag aaaggaggca ctaacggtta aatagcccga tgaaaggaat atcatcgggc
    10441 ataaggcgat tatgcgagaa ccaaatcccc ctgcggatgg caggagcagg ccagtacgta
    10501 accttcagcg atttcggcgt cggtcagcgt cattgtactg ctcaccgtat attcaccgga
    10561 aaccactttt gtcttacagc agccgcaaac acccgcacgg caggcagcga caaccggaac
    10621 gttattgctt tccagcgcct ccagtagtgt ggtgccaacc ggggcgtaaa attctcgtgc
    10681 cggttgcagt ttggtgaatt tcagaccgct ggtcgccgct tttgctactg gggtgaagaa
    10741 tttctcttta aagaaacgcg tcacgccgag cgctttcact tcctgctcta cccaatccat
    10801 atacggagcc gggccgcagg tcatcacggt acgtgaagct aagtcaggta cacctgccag
    10861 cagttctcga gtgagacgac cagcgataaa gccttcggta acgttatttt ctgccaccag
    10921 cgttaccgga tagttacgcc actcatcggc gaaaataaca tcctgcggcg tatgcacgtt
    10981 gtagatcacc cgcacatcgg cctgtggacg gttcttcgca agccagcgac gcatcgacat
    11041 aatcggcgtg acgccgcagc ctgccgccag caacaggaat ttatcttctg ctttatcgtc
    11101 gcaggtaaat tcccccatcg cgtccgaaag ccagagataa tcaccgcgtt ttacatcgcg
    11161 cgtcagccac tgggagccga caccgtcatc aatccgccgc acggtcaggg tgatatattc
    11221 actcacgcct ggcgtggagg aaatggtgta agcacgcagc gtttccgctg agtttcgcac
    11281 gctgaccagt gcatattgcc cggcgcgata tgggtagtaa tcgtggcaaa tcagggaaat
    11341 cgtccacaca tccggcgttt cttgcgtaat gtgatgaacc tgcatccgcc acgggcattg
    11401 attcgttggc atcgtcatcg acaaactcct tacgcgctca acagttgctt catgtcttct
    11461 tcaacagtgg tgatagaacg caggccgaat ttctcgttca gcaccgccag caggtctggt
    11521 gtcaggaaac caggtgcagt cgggccggtg acgatatttt tcacgcccag agaaagcagc
    11581 gtcagcagaa tgacgatcgc tttctgttca aaccaggaga gcaccagcga cagcggcaga
    11641 tcgttcacac cacagcccag tttctctgcc agagtgacag ccagaataat cgctgagtaa
    11701 gcatcgttac attgacctgc atctaccaga cgcggcagac cttcgatatc gccaaactca
    11761 agtttgttaa agcgatattt accacaggcg agggtcagga tcaggcagtc atccggcacg
    11821 atggtggcga aatcggtgaa gtagtggcgc tcgccgcgtg cgccgtcaca gccaccaagc
    11881 aggaagatat gacgcagttt ttcacggctc accagatcaa tcagcgtatc agcagcgcca
    11941 agcagcgtct ggcgaccaaa acccacggtg ataaggtgcg gaatttcgct gtacgggaag
    12001 cctgccatct gttgcgcctg ggtgataacc gcagagaaat catcaccgtc cagatgacgc
    12061 acgccaggcc agccaacaat gctgcgggtc cagatacgat cgtcataagc gcttacggtt
    12121 gggtcgatga tgcagttcga ggtcatcacg atggggccag ggaaacgagc gaactccact
    12181 tgctgattct gccagccgct gccgtagtta ccgaccagat gcttgaattt acgcagctcc
    12241 gggtagccat gcgcaggcag catttcgccg tgggtgtaga cattaacgcc cgtgccttcg
    12301 gtctgttcca gcaggttgta gagatctttg agatcgtgac cggaaatcag aatgcattta
    12361 cccgccgtcg ctttgacgtt gacctgggtt ggcgtcgggt gaccgtattt accggtttcg
    12421 cctgcatcca gaatgctcat cactttgaag ttcatctggc cgatttccat tgaacactca
    12481 agaagcgcgt tcatatcggc aggccaggtc cccagccacg ccatgatttt atggtactgg
    12541 gcataaatat cgttgtcgta ttgaccgaga acatgcgcgt gttccatata ggccgccgca
    12601 cctttcaggc catacaggca cagcagacgc aggccgagaa tgttttcgcc aatcgccgct
    12661 ttatctttgt taggggtaaa ttctgctgcc tgacgttgca gctcgccgag atcatcgctc
    12721 accagttgca ggtcagccat cgggttatcg acgcgcgcgt tggcatctac agccaggcat
    12781 tgtgctttca gcgcctcgcg caaggcaatc gcttcacgag cgtagccgac aatacgcgga
    12841 gaatcgaagt taacgttggt cagggttgag aaaaaggcac gtggcgcgaa gctgtcaaca
    12901 tcgtggttga tgatgccgta ttcacgcgct tttaccgccc aggcagaaag cccttgcagc
    12961 gccgcgatga gtaaatcctg aaggtcagaa gtttccgccg ttttaccaca catcccctgc
    13021 gcgtatgagc agccgtttcc tgccggagta cggatagttt gttcacattg cacacaaaac
    13081 atgatcacac cttttaaagt tatatttaat atacatgttt aaggttaaga cgcttaacgc
    13141 ggggataaaa gggatttttc atgcaacttt aagggagatt gatttagcgc aattttggcg
    13201 gcagggatct accgccagag aggtattacg cagagaaaaa ggcgatgagg atcggcacta
    13261 acaggctaag aataaaaccg tgaacaattg ccgccgggac catatccagc ccgccagtac
    13321 gttgaagaac gggcagggtg aaatccattg atgtggcacc gcataagccc agtgcagtag
    13381 agcggctgcg gcgaatcagc ccagggatca acataatagc aatcagttca cgggccagat
    13441 cattaaaaaa cgccgcgctc ccgattaccg gaccaaaaga ttcggtcaat aaaataccgg
    13501 aaagagaata ccagccgaaa ccggaggcca ttgccagcgc ggtattgatg gggagatcaa
    13561 gaataaaggc gttaattaaa ccaccaatta atgaactgac aaccaccacc acggcgacaa
    13621 tcattccccg gcgattaagg acaatctgct ttaaggtcat gccattattg cgcaactgaa
    13681 taccaacgag gaaaagtagc aaaattaacg tgtattcact ggcttcggtc gcgtgttgta
    13741 agaaagccag tccactcaga ccaatggcaa aaccaatcac tactacgccg cacagtttta
    13801 gcgactccag cgccatcgca atacgcgacg ggagtttttc ttgctgatgg tggttgcgcc
    13861 acggcaggcc tcgctccagc cacatcaggg cggcaatatt acacagtaaa ataacggtaa
    13921 tactgacggc agaataatgc agaatcgcca acaggttact ggcgaggtta tcgagaaacg
    13981 ccagactgat acccataaaa aagagaataa ggtaaaccat ccagcttaat agctgattaa
    14041 taacttttaa cgcagcttgt tggcgaagcg gaatgaggta acccacaatc aggggaacca
    14101 gaatgattaa cagcccagaa aacatgaaaa cccagtcctt gcaaagatga agtcgaaatg
    14161 cgcgatgaca cactactgaa agcggaagga cgagtaaagt tgcaattaaa aggaaatgtt
    14221 atgcataagg agcagtagag tattcgtttt catttaaaga tattcttgcg ctttaattac
    14281 aaactgcacc gatgttggtg gcgtcaaaat cgccgaggcg ttccctgaag gccggggcag
    14341 cccacatgga tgtgggctga gggcgcgttt tacagggatg ttacctcgcg cccgacccgg
    14401 tagccgtaag ggataagtcg agggcaccgc gcagcggcga ttttgttcgc cagagcccgg
    14461 gggggcaggg ggcggcggcg attggccgcc ccctgcgcgc tccttgcgcc agtggcaata
    14521 tgtggcttag ctcatgaaag gagcgcaaca agatgatgaa tcaacatata acaacatctt
    14581 aaaaaaagcc tgacattacg ccaggccttc tgagttaatt aatcacgctt ttccagcagg
    14641 gtccggtaaa tcagaccacc gataatgccg ccgacaattg gcaccaccca gaagaaccac
    14701 agttgttcta atgcccagcc gccctggaag atagcaaccg cggtgctgcg cgccgggtta
    14761 acagaagtgt tagtcaccgg aatactaatt aagtgaatca gggttaaggc cagaccaata
    14821 gcgatcggcg caaaacctgc cggcgcgaat ttggcggttg cgccgtggat caccaacagg
    14881 aaacctgcac tcaataccag ttcaactacc agcgcggaaa gcatggaata accgcctggt
    14941 gaatgctcgc cataaccgtt agaagcaaaa ccgctggctg ccgcgtcaaa acccgtttta
    15001 ccactggcaa ttaaatacag cagcgccgct gcaacaatac cgccgacaac ctgggcaatt
    15061 acgtagccaa cgacttcttt tgccggaaaa cgtccgccag cccataaacc aatagtgacc
    15121 gccgggttaa aatgaccacc agaaatatga ccaacagcaa aggccatcgt cagaacggtc
    15181 agaccgaacg ccaacgccac gccggcaaaa ccaatgccta attccgggaa gcctgcggcc
    15241 agtacagcac taccacagcc accaaaaaca agccagaaag taccaaaaca ttcagctgct
    15301 aattttctga acatatccac ctcaattaaa aattgaccct gtgaaaaata tggtcgtttt
    15361 atagggtcgt cgtaaaaaaa tgacgacgga aataatgcgc ggctatttta aaaacgaagg
    15421 cgagtcattc accagataaa taaatccagt aaatttgatt tagggcaaca gcgggttgcc
    15481 ccatatagtc atttgtctga ttgacagtgt agtgcacgca aaagatttaa tcctttaggc
    15541 gtaataaaaa ataatatatc atgctaatta tttgattttg ttgtttttgc agacttatca
    15601 gcaagaggga gtataacgcg attattcgct catttttcag acatttgcca tgcttaaatg
    15661 tgatgtcatc acgtattagc aaggcctttc ccgttatact gccagcgtaa aggataagtc
    15721 acatatttct ggaggggata tgattcttga gcgcgttgaa attgtgggtt ttcgcggtat
    15781 caaccgtttg tcgttgatgc tggaacaaaa caacgtcctg attggggaga acgcgtgggg
    15841 taaatccagc ttgctggacg ccttaaccct gctgctatcg ccagaatcag atctctacca
    15901 ttttgagcgc gacgatttct ggttcccgcc gggagatatc aacgggcgag aacatcatct
    15961 gcatattatt ttgaccttcc gcgaatcgct gccaggccga catcgggttc gccgttatcg
    16021 gccgctggaa gcgtgctgga cgccatgcac cgatggctat caccgtattt tttatcgtct
    16081 ggaaggggag agtgcggaag acggcagcgt gatgacactg cgcagttttc tcgataaaga
    16141 cggacatccg attgatgtcg aggatattaa cgatcaggca cgccatctgg tgcgtttaat
    16201 gccggtgctg cgcttgcgtg atgcccgttt tatgcgccgt attcgtaacg gcacggtgcc
    16261 aaatgtccct aatgtggaag tcaccgcgcg ccagctcgat ttcctcgccc gtgagttatc
    16321 ctcacatccg caaaatctct ctgatgggca gattcgtcag ggactttccg caatggtaca
    16381 gctgcttgag cattatttct ctgagcaggg ggccggacag gcgcgatatc gtttaatgcg
    16441 gcggcgggcc agcaatgagc aacgaagctg gcgctatctg gacatcatca accggatgat
    16501 tgaccgacct ggagggcgct cgtatcgggt tattttgctc ggcctgtttg ctactttatt
    16561 gcaggcaaaa ggcacattga gactggataa agacgcgcgt ccattgttgc tgatcgaaga
    16621 tccagaaacc cgtttacacc ccattatgct ttcagttgcc tggcatctgt tgaatcttct
    16681 gccattgcag cgcattgcca ccaccaactc gggtgagttg ctttcgttaa cgccggtaga
    16741 gcatgtttgc cgactggtac gtgagtcctc gcgcgttgcc gcctggcgtc tggggccgag
    16801 tggcttgagt accgaagata gccgacgcat atcctttcac attcgtttta accgtccgtc
    16861 atcgctgttt gcacgctgct ggttgctggt ggaaggggaa acggaaacct gggttatcaa
    16921 tgaactggcg cgtcagtgcg gacatcattt tgatgccgaa gggatcaagg tcattgagtt
    16981 tgcccagtcc gggctaaagc cactggttaa atttgcccgc cgaatgggga ttgaatggca
    17041 tgtactggtc gatggcgatg aagcagggaa gaaatatgcc gctacggtac gcagcctgtt
    17101 gaataacgat cgggaagccg aacgagaaca tttaacggcg ttaccggcgc tggatatgga
    17161 acattttatg tatcgccagg gattttccga tgtgttccac cgcgtggcgc aaatcccgga
    17221 aaatgtaccg atgaatctac gcaaaattat ctcgaaagcg atccatcgct cttccaaacc
    17281 cgatcttgcc attgaagtgg caatagaggc aggacgtcgt ggtgtggact ccgtaccgac
    17341 gctgctgaaa aaaatgttct cacgcgtgct gtggctggcg cgcggtcgcg cggattaacc
    17401 gcgaaacatc gtggccattt gtggctgaat agcgtcgagc atctcatagc gccgacggta
    17461 ttcagcccgt tttttactgg cgatttcggc aatctctttt cgtgctatct gtgctggaag
    17521 gcggtaatgg cgttcagcat cacatacgcc gccaaccgat tcccagaaag cgttgtaatc
    17581 agcgtggatc ttgccttctt tatcgcgata acgcaggctg cggtaaatat gcgtttcatt
    17641 gctgacggca ataatctgct ctacctgcaa acgttgggca aacagacagg ccgcttccat
    17701 cacgaggcgt ttgggaaata gcccgtggca ggctttcgtc gcattctgga tttcctgatg
    17761 tggaatttcc cattttgcgc cttgcagtcc gccaataaac atcgttcttt tcccctgata
    17821 ttcacacagg gtaaacgtga tctctgccag aggaatacct tcgctgttgc gaaacaggat
    17881 tgtgctgtca ccttctttat ccattgagat catcatggtc agcttaagcg tgaactgctc
    17941 gccgtttttg ccttccagct tcgccagttg cagcccgggg gtattcaaat ataagctgaa
    18001 ttcttccgcc gacatacatc cgcggagtaa cgcataatgg taacgtaacg cctccagcaa
    18061 ttgcttacgg ctaagattcg ccgcaaggta agggcgatgc agacgcacag gcagtcgcgg
    18121 ctggcgcgtt aacaacacat tgagattagg ccagtgggaa agttcgttca tccactcaac
    18181 gcttaaacgc ggcataatca acgagcgcag caaaaatttc tggcgaaaac tacggcgatg
    18241 ccagaattta cccggccgac actgtccacg tgccagacta agaaaaagtg acaggctgct
    18301 gagagattca gatggcgtaa aggtccgttc agttagctgc gacatattca tgaaatcatt
    18361 ggttatacat gacgtcgatt tcaccattgc gtatcttaac caaacatcaa tagtgtgatt
    18421 actaacgtaa attttagggt tttgttgata tttcgttgaa gttaatgacc cggattggca
    18481 tatggagtat tcagaatatt tatgaaaaag cggaaaaccg tgaagaagcg ttacgttatt
    18541 gcgctggtga tagtcatcgc cggactgatt acgttatgga gaattcttaa cgcacccgtg
    18601 ccgacttatc agacactgat tgtgcgcccc ggtgatttac agcaaagcgt gctggcgacc
    18661 ggaaagctgg acgcgctgcg taaggttgat gtgggcgcgc aggtcagcgg tcagttgaaa
    18721 actctgtcgg tggcgattgg cgataaagta aaaaaagacc agcttttagg ggttattgat
    18781 cctgaacagg ctgaaaacca gattaaggag gtcgaagcaa cgctgatgga gctacgtgcg
    18841 cagcggcagc aggcggaagc ggagctgaaa ctggcgcggg tgacgtattc ccgtcagcaa
    18901 cgtctggcac aaacgcaggc ggtttcacag caggatctcg acaccgccgc gacggagatg
    18961 gctgtgaaac aggcgcaaat tggcaccatt gacgcggaaa tcaagcgcaa tcaggcttct
    19021 ctcgatacgg ctaaaaccaa tctcgattac actcgcatcg ttgccccgat ggccggggaa
    19081 gtcacgcaaa tcaccactct gcaaggccag acggtgattg ccgcacaaca agcaccgaac
    19141 attctgacgc tggcagatat gagcaccatg ctggtaaaag cgcaggtttc tgaagcggat
    19201 gtaatccacc tgaagccggg gcaaaaagct tggtttacgg tgcttggcga tccactgacg
    19261 cgctacgagg ggcaaatcaa ggatgtacta ccgacgccgg aaaaggttaa cgacgctatt
    19321 ttctattacg cccgttttga agtccccaac cccaatggtt tgctgcggct ggatatgact
    19381 gcgcaagtgc atattcagct caccgatgtg aaaaatgtgc tgacgatccc tctgtcggcg
    19441 ttaggcgatc cggttggcga taatcgttat aaagtcaaat tgttgcgtaa tggtgaaaca
    19501 cgcgagcgtg aagtgacgat tggcgcacgt aacgataccg atgttgagat tgtcaaaggg
    19561 ctggaagcgg gcgatgaagt ggtgattggt gaggccaaac caggagctgc acaatgacgc
    19621 ctttgctcga attaaaggat attcgtcgca gctatcctgc cggtgatgag caggttgagg
    19681 tgctgaaggg catcaccctc gatatttatg ccggtgagat ggtagcgatt gttggcgctt
    19741 caggttccgg taaatcgacc ctgatgaata ttctcggctg tctggataag gccaccagcg
    19801 gcacctatcg cgtcgccggt caggatgttg ccacactgga cgccgatgcg ctggcgcaac
    19861 tgcgccgcga gcatttcggc tttattttcc agcgttacca tttactttcg catttaaccg
    19921 ccgagcagaa cgttgaagta cccgccgtct atgccggtct tgagcggaaa cagcggctgc
    19981 ttcgcgccca ggagttactg caacgactgg ggctggaaga ccgtacagag tattatccgg
    20041 cacagctttc gggtggtcag caacagcgcg tcagcatcgc gcgggcattg atgaacggcg
    20101 gtcaggtaat tcttgccgat gaaccaaccg gcgcactgga cagccattct ggcgaagagg
    20161 tgatggcgat cctgcatcag ctgcgcgatc gcgggcatat ggtgattatc gtcacccacg
    20221 atccgcaggt cgctgctcag gccgagcggg tgatcgaaat tcgcgacggc gaaattgtgc
    20281 gcaatcctcc cgccattgaa aaagtgaatg ttgctggcgg gacggagcct gttgtcaaca
    20341 cggtgtctgg ctggcggcag tttgtcagcg gttttaacga ggcgctgacg atggcatggc
    20401 gggcgctggc agcgaataaa atgcgtactt tactgaccat gctggggatt attatcggta
    20461 ttgcgtcggt ggtttccatt gtcgtggtgg gtgacgccgc caaacaaatg gtgctggcgg
    20521 atattcgttc tattggtacg aatactattg atgtctatcc cgggaaagat tttggcgatg
    20581 acgatccgca atatcagcag gcgctgaagt acgacgactt aatcgccatc caaaaacaac
    20641 cgtgggtcgc ctcagccaca cctgccgtct cgcaaaacct gcgcctgcgt tataacaatg
    20701 ttgatgttgc tgccagtgcc aatggcgtga gcggcgatta ttttaatgtc tatggcatga
    20761 ccttcagtga aggaaacacc tttaatcagg agcagctgaa cggtcgtgcg caggtcgtgg
    20821 ttctcgacag taatactcgc cgccagcttt tcccccataa agcagatgtg gttggcgagg
    20881 tgattctggt cggcaatatg cccgccagag tcattggtgt ggcggaagaa aaacagtcga
    20941 tgtttggtag cagtaaagtg ctgcgtgtct ggctacctta cagcacgatg tccgggcgag
    21001 ttatgggcca gtcgtggctg aactccatta ctgtcagggt aaaagaagga tttgacagcg
    21061 ccgaggcgga acagcaactc acgcgtttac tttcactgcg ccacggaaag aaggatttct
    21121 ttacctggaa catggacggc gtcttgaaaa ctgttgaaaa gaccacacgt actttacaac
    21181 tgtttctgac gctggtggcg gtgatttcgc tggtggtggg cggtattggt gtaatgaata
    21241 ttatgctggt gtcagtgacc gagcggacgc gggaaattgg cattcgcatg gctgtaggtg
    21301 cgcgagcaag cgatgttttg caacagttcc tgatcgaagc cgtactggtt tgcttggtcg
    21361 gtggcgcgtt gggaataacg ctgtcactgt taattgcttt caccttgcag cttttcttac
    21421 ccggctggga gattggtttt tcaccgttgg cactgctgct ggcgtttctc tgctcgacgg
    21481 ccaccgggat tttatttgga tggttacccg cacgaaatgc ggcacgactg gatccagtag
    21541 atgctctggt acgagagtaa tttttgagat aaaaatgcca gccgatcggg ctggcatttt
    21601 gcctttagga tgtacacaat gagacagaag agctatgcga ctgccgcttc tacttcgacg
    21661 ggcacaataa cactggcgtg attgcctttt ggcccctggt ggacatcaaa ctgaacggat
    21721 tgtccagctt ttagcgttct gtaaccatcc atctgaatgg tggaataatg agcgaaaata
    21781 tcttcgccgc cgccttcagg gcagatgaaa ccaaaccctt tggcattgtt gaaccactta
    21841 acagtaccct tttccatgct tcgacatcct tcgcaaatct tatacaagta agatggaata
    21901 aaccggggtc agagaggggg atgttcaaaa cctcgccaac tctagaaata caatttagag
    21961 aattagggcg agccgtcaag cgtttgacag gggccagggg gcaggtatga atcaaaaatt
    22021 tgaagcagtt aacgctattg acaggaatgt gacagatgtc gctgatgcca acgatagatg
    22081 atagttatct atcatgtgga gtagattggt caggcaaata agctcttgtc agcggcaggg
    22141 cgttctgccg ataaccgtaa ccgaagatga taactgacaa tgggtaaaac gaacgactgg
    22201 ctggactttg atcaactggc ggaagaaaaa gttcgcgacg cgctaaaacc gccatctatg
    22261 tataaagtga tattagtcaa tgatgattac actccgatgg agtttgttat tgacgtgtta
    22321 caaaaattct tttcttatga tgtagaacgt gcaacgcaat tgatgctcgc tgttcactac
    22381 caggggaagg ccatttgcgg agtctttacc gccgaggttg cagaaaccaa agtggcgatg
    22441 gtgaacaagt acgcgaggga gaatgagcat ccattgctgt gtacgctaga aaaagcctga
    22501 atgcaggcat aaaaattggg ggaggtgcct atgctcaatc aagaactgga actcagttta
    22561 aatatggctt tcgccagagc gcgcgagcac cgtcatgagt ttatgaccgt cgagcacttg
    22621 ttactggcgc tgctcagtaa cccatctgcc cgggaggcgc tggaagcgtg ttctgtggat
    22681 ttggttgcgc tccgtcagga actggaagcc tttattgaac aaaccacacc cgttctgcct
    22741 gccagtgaag aggagcgcga cacacagccg acgctgagtt ttcagcgtgt actgcaacgt
    22801 gcggtcttcc atgtccagtc ctccggtcgc aatgaggtaa ccggtgcaaa cgttctggtc
    22861 gctatcttta gcgaacagga gtcgcaggcg gcatatctgt tgcgtaaaca tgaagtcagc
    22921 cgtctcgatg tggtgaactt tatctctcat ggcacgcgta aagacgagcc gacacagtct
    22981 tctgatcctg gcagccagcc aaacagcgaa gaacaagctg gtggggagga acgtatggag
    23041 aatttcacga cgaacctgaa tcagcttgcg cgcgtgggcg gaatcgaccc actgattggt
    23101 cgtgagaagg agctggagcg tgctattcag gttctctgcc gtcgccgtaa aaacaacccg
    23161 ctgctggtgg gggaatctgg tgtcggtaaa accgcgattg cggaaggtct tgcctggcga
    23221 attgttcagg gcgatgtgcc ggaagtgatg gctgactgta cgatttactc tctcgatatc
    23281 ggttctctgt tagcgggcac taaatatcgc ggcgactttg aaaaacgttt taaagcgttg
    23341 ctcaagcagc tggagcagga cactaacagc atcctgttta ttgatgagat ccacaccatt
    23401 atcggtgcgg gtgcagcgtc tggtggccag gtcgatgcgg ctaacctgat caaaccgttg
    23461 ctctccagcg gtaaaattcg cgtaattggt tcgacaacct atcaggagtt cagcaacatt
    23521 ttcgagaaag accgtgctct ggcgcgtcgc ttccagaaaa ttgatattac tgaaccgtcg
    23581 atcgaagaaa ctgttcaaat catcaatggc ctgaaaccga agtatgaagc gcaccacgac
    23641 gtgcgttata ccgcaaaagc ggtgcgtgca gcggtagagc tggcggtgaa atacattaac
    23701 gatcgtcatc tgccggataa agccattgac gttatcgacg aagcgggcgc tcgcgcacgc
    23761 ctgatgccgg taagcaaacg caagaaaacc gttaatgtgg cggatattga gtccgtggtg
    23821 gcccgtattg cgcgcattcc agagaagagt gtttctcaga gtgaccgcga taccctgaaa
    23881 aacctcggcg atcgcctgaa aatgctggtc ttcggtcagg ataaagccat tgaggcgctg
    23941 actgaagcca ttaagatggc gcgtgcaggt ttaggtcacg aacataaacc ggttggttcg
    24001 ttcctgtttg ccggccctac cggggtcggg aaaacagagg tgacggtaca gctttcgaaa
    24061 gcgttgggca ttgagctgct gcgctttgat atgtccgagt atatggaacg ccataccgtc
    24121 agccgtctga ttggtgcgcc tccgggatac gttggttttg atcagggagg tttgctgact
    24181 gatgcggtca tcaagcatcc acatgcggtg ctgttgctgg acgaaatcga gaaagcgcat
    24241 ccggacgtgt tcaatattct gttgcaggtg atggacaacg gtacgctgac cgataacaac
    24301 ggacgcaaag cggacttccg taacgtggtg ctggtgatga ccaccaacgc cggggtacgt
    24361 gaaactgagc gtaaatccat tggtcttatc caccaggata acagcaccga tgcgatggaa
    24421 gagatcaaga agatctttac accggaattc cgtaaccgtc tcgacaacat tatctggttc
    24481 gatcatctgt caactgacgt gatccatcag gtggtggata aattcatcgt cgagttgcag
    24541 gttcagctgg atcagaaagg tgtttctctg gaagtgagcc aggaagcgcg taactggctg
    24601 gccgagaaag gttacgaccg ggcaatgggc gcacgtccga tggcgcgtgt catccaggac
    24661 aacctgaaaa aaccgctcgc caacgaactg ctgtttggtt cgctggtgga cggcggtcag
    24721 gtcaccgtcg cgctggataa agagaaaaat gagctgactt acggattcca gagtgcacaa
    24781 aagcacaagg cggaagcagc gcattaatct gattgttagg taggttggtc aagtccgtaa
    24841 tctcgaaaga ggttacggac tttttgttta tggggtggag gaggttcaga cccttttttt
    24901 aatgatgatg gtaagttgtt gataattagt gctgcgggaa ggtaaggaca aaaaagggtg
    24961 ctgcaggaga atgggatggt tttgctttat ttacaacggg ctaaacgtgt agtatttgag
    25021 ttcactgccg tacaggcagc ttagaaattc acaggtaaca tactccaccc gcccaccatg
    25081 ttcactgccg tacagacaga taaaatgcga aaaaaaagct cgcactttcg tacgagctct
    25141 tctttaaata tggcggtgag ggggggattc gaacccccga tacgttgccg tatacacact
    25201 ttccaggcgt gctccttcag ccactcggac acctcaccaa attgttttgc taccaaacct
    25261 catgggtggc aacggggcgc tactataggg agttggagta aaacggtcaa gaagaatttt
    25321 aatgataatt attgtttgct catactgtaa acaagttgtg cagtatatct acatcgagac
    25381 aagttacgga cttatacttc caaagtactt catacatatc acaaaataaa aaggccggtt
    25441 aaaccgacct tttactcgtt ctttctcttc gcccatcagg cggtaaaaca atcagcgact
    25501 acggaagaca atgcggcctt tgctcaggtc gtacggggtc agttcaacag tcactttgtc
    25561 gcccgtcagg atgcggatgt agtttttgcg cattttaccg gagatgtgtg cagtaaccac
    25621 gtgaccgttt tctaactcta cgcggaacat ggtattaggc aacgtttcaa gaacggtacc
    25681 ttgcatttca atattgtctt ctttggccat ctaatcctct ggggtatcac taccgtaatt
    25741 tgaaccggca agataatgcc gaagttctgt aaataagtaa agatttgcgc gctaaatcgc
    25801 aacaaacagg ttcggcacat tactccgaaa acacacggct aagccgcacc aaaagcgcaa
    25861 cgtataaggg agcggtgaga taaacgatgg gcgttacctg acgcgaaaaa ttccttatcg
    25921 gcagcggggt aatgagcgta accaactctg cgaccgcaat tataacactc tggggagaaa
    25981 tgtgccgaaa acattcattc ttgtggtgaa aacaagcatc gtggtaccca gaaattattc
    26041 ggcaatcgtc cgaggcgcat ttgattgaga taattaaggt aatcccggcg gggaatttcg
    26101 caggcaccaa gcgatgctgt gtgatcgtta aggacctggc agtcgataag cttaccgcca
    26161 tgaccgataa actcctcaca gaataccaga agcgccgttt tagacgcatt ttccatccgg
    26221 ctgaacatgg actcgccaca aaatagcgtt ccctgggcca cgccgtacat accgccgaca
    26281 agctcatctt cacgccagac ttcaatggag tgggcatggc cgagttcgtg aaggcgatgg
    26341 taggcttcga ccacgccacg cgtgatccag gttccttctt cgcgatcgct ggcacagcct
    26401 tcaatgacct gaccaaaagc gtaattcatc gtgacacgat agggcgagcg tttatgaaat
    26461 cgcttcatac tacggctgat atgcagtgat tctggccata gcaccgcgcg gggatcgggc
    26521 gaccaccaga ggatggggtc gcctggagaa aaccacggaa aaataccacg ctggtaagcc
    26581 attaacaggc gcgcagggct aagatcgccc ccaagtgcca gcaggccgtt aggctcacgt
    26641 aatgcgcctt ccggggaagg gaaggctatt gaatggcgag aaagctgaac caggcgcatg
    26701 accgcaaaac tccacgcaag tcggatcgtt caataatagc ttacaaaccc tgcttgaact
    26761 ggtaataacg cccctgtctg gcaagcagct ctgcgtgagt accttgctca ataatttgcc
    26821 cgttgtccat cactattatt tgttggaaac gagagagtcc gcgaagtcga tgggtgacca
    26881 ttaacaccgt tttctcacgc atcatttctg caagcaattc aaggatctgg ctttcggttg
    26941 tggcatctaa gccttcggta ggttcatcca gcaacaccag tggcgcatca tgtaacagcg
    27001 cacgggcgat agccagacgg cgcagttcac caccggagag ctggcgtccg ccttcaccta
    27061 accaactgtt gagacctgca tcctcgagca gcttctccag accaacgcga cgcaagctct
    27121 ccgccagagc ctcatcacta ctgccaggcg aggcgagtaa cagattatca cgcagcgtgg
    27181 cgctaaacag atgcactcgc tgaggaacaa cgctgatggt ctgtcgtaga gccgcttcat
    27241 tcaggctggc tatggggcta tcgttaagca aaatctcgcc ctgttgcggg tcccaagcgc
    27301 gggtcagcag ttgtaacagt gttgatttgc cgcatccggt tcgcccgaga atcgcaatat
    27361 gttccccggc gtttacctga agagaaatcc ctttaagtgc ctgttgggat tgctccggat
    27421 aagtgaactg aacatcccgt aatgtcagcg aaacgcgatc ggcaacacga gtttgggtat
    27481 cagggaaggt gacttccggt ttttgatccg ttaaatcagt gatccgaacg gcagaggcaa
    27541 tgacttgccc cagatgctga aatgcacccg ttactggtgc cagtgcttca aacgcggcta
    27601 acgcgcagaa gacaaacagg gcaattaacg cgccgggttg agcattgccg ccaacgccgc
    27661 cagacgccat ccacagcatc aggatcaccg ctaacgcgcc aatgagcagc attatcgctt
    27721 gcgacaatgc ggtcagttca gattgacggc gttgcgcttc cagccattga atttctgtat
    27781 tctctagttg cgtgcgataa cgatcgctgg caccaaaaat ggtcagctca gcttgccctt
    27841 gcagccaggc cgtcagttgt tggcgatact gtccgcgaag atgagtcaga ttttgcccgg
    27901 tgctttttcc cgcacgataa aacagcggtg gcatcaggaa aagcgtcagt aacataatgc
    27961 cgcccagcgt aaaggcgagg gtgaaatcaa ggaaacttaa cccgattgtc accatcataa
    28021 tcaccacaaa agcgcccacc agcggcgaga taacgcgcag gtaaagatga tcgagcgtat
    28081 caacatccgc caccacgcga ttgagcaatt cgccctgacg atagcgcgcc agtccggcag
    28141 gggagagggg caacaatttg ctgaaggtgt aaatgcgcag atgctgcaac acgcggaaag
    28201 tcgcgtcgtg acttaccaga cgttcaaaat aacgcccggc agtacgggtg attgcggcac
    28261 cacgcacgcc cgcagcgggt agcatatagt tgaagctgta cagtccggca acccccgcaa
    28321 ccgctgaggc cgagaggaac cagccggaaa gtgtcaacag tccgatactg gcgagcagcg
    28381 tcacaattgc cagcacaata ccaagactta acatccattt atgacgttta tacagtgcta
    28441 gatagggtag caaagcgcgc atttaaatct cctcctgacg atgggccagt aatgtggcga
    28501 atgggccacc agccacactt aattccgcgt aacgtccttg ctcaataatc cggccatcct
    28561 gcataaccca aatgacatcc cagtcagcaa gatcttctaa ctggtgggtg accattaacg
    28621 ttgtctggcg cagagaggcg gcattcagcg cctccattac gcgctgttca ctgtgagcat
    28681 caaggctggc agcgggttca tccaacagta atagcgaaca gggatttagt aacgcacggg
    28741 ccaccgccac gcgctgcgcc tgccccacgg aaaggcgggc agcctggtcg ccaacaggcg
    28801 tatcaacgcc ttgtggcagg agcggtagaa actcgctgac ccaggcgtta tccagcgctg
    28861 cttgtaattc atgttcgctg gcatcaggtc gcgccagtag tacgttatcc cgtaatgttg
    28921 ctgccggtaa ttgtggattt tgcccaaccc aggagagatg tttacgccat gattctggtg
    28981 ataaatcgcg taattctatc ccgttgattc gtagcgatcc ctgatatgag agaaaaccag
    29041 aaagcgcgtt caacagtgaa cttttacctg aaccgctgcg accaaccaac acggcacgtt
    29101 ggcctgctgg caaagtaaag ttcagcggtc cggccagcgt tttaccttcc ggcgacgtga
    29161 taaacagatc ctcggcctca atggtcaccg gatcggtcga tgctaattcc acctcgccac
    29221 actgcggatg ggcgagcggg gtttccataa acgttttcag actgtcagct gcaccaacag
    29281 cctgggcttt agcatgataa aacgtaccga gatcgcgtaa tggctggaaa aactctggcg
    29341 caaggatcag ggccagaaaa cccgcagcca gcgtcacgcc agtatcgtag tgaccaaaat
    29401 ccagctcgcc aagataggaa aaaccaaagt agaccgccac cagagcaatt gacagcgagg
    29461 taaaaaattc gagaatgccg gaggataaaa atgccagccg tagcacttcc attgtccgtt
    29521 ggcggaaatc ttccgaagca gaacgaatac tttcaatttc agcttcacca cgaccaaaaa
    29581 tacgcaatgt ttccatgccg cgcaggcgat cgaggaaatg cccacttaag cgagcaagag
    29641 cgagaaagtt acgtcggtta gcatcggcag cccccattcc aaccagcgcc ataaacaacg
    29701 gaattaacgg tgcagtgccc agcagaatga gcgccgcagc ccagtttgag gggaagattg
    29761 ccaccacaat cagcaacggc accgacactg ccagcgccat ttgcggcagg tagcgtgcat
    29821 agtaatcatg catatcgtca atttgctcga gtaccagcgt cgcccagctc cccgcaggtt
    29881 taccctgaat ccacgctggc cctgcttgtt gcagacggtc gagaacctgc cggcggatgg
    29941 caaagcggat atgctgcccg gcgtgataac ccacccgttc gcgtaaccag accacccatg
    30001 cgcgcagtac aaaggtcaga accagtaacg taaagggaag cagcagggct tcacggggaa
    30061 tattctccat aatcatatgt tgcagaattc gcgccatgaa ccaggcctgg gcaatgatca
    30121 atatgccgct cacaaagccc agcagacgag aaatattcag ccaacgttgg gagatgacgc
    30181 tttgctgttt taaccagcgg gttaactctt tttgacgaga tttattcatt gcacgcttag
    30241 caggtgagtt atcagaatta tttgcagagc aatgttacaa cggggaaaaa ataaaggcga
    30301 cccatagtcg caaggtgtcg ccttctttac ttttgttact gatttgtaaa attattttgc
    30361 gtcagctaaa ccatcgaggt agcgttccgc atcaagtgct gccatgcagc ctgtaccggc
    30421 cgaagtaatg gcctgacgat aaatatgatc catcacgtca cccgcggcaa agacgccagg
    30481 aatgctggtc tgggtggcat taccatgaat acccgactgt actttgatgt agccgttttc
    30541 cagttccagc tgcccttcga aaatggcagt attcgggctg tgaccgatag caacaaacag
    30601 accggcaacg tcgagtgatt cgatgttatc gctgttttgc gtatcgcgca gacgaacgcc
    30661 agtgacaccc atttgatcgc cggtcacttc ttccagcgtc cggttggtgt gcagaatgat
    30721 gttgccgttc tccactttat ccatcagacg cttaatgagg attttttccg cgcggaaacc
    30781 gtcacggcgg tgaatcagat gtacttccga agcgatgtta gacagataca acgcctcttc
    30841 aaccgcggta ttgccgccgc cgatgaccgc aactttctgg ttgcgataga agaaaccgtc
    30901 gcaggttgca caagcagaaa ccccacggcc tttaaaggct tcttcagagg gcaggccgag
    30961 atagcgtgca gaagctccgg tggcaataat cagcgcgtcg caagtgtatt cgccgttatc
    31021 gccattcaga cggaacggac ggttttgcag atccaccttg ttgatatgat caaaaatgat
    31081 ttcagtttca aatttggtgg catgttcgtg catgcgctcc attaataacg gaccggtcag
    31141 atcgtttgga tcgccaggcc agttttccac ttctgtggtg gtggtcagtt ggccgccttt
    31201 ttccatgccg gtaatcagca caggttgcag gttggcgcgc gccgcataga cagcagcggt
    31261 gtatcccgcc gggcctgaac ccaggataag cagtttactg tgtttggtcg tgcccatgag
    31321 atccccatag ttgttggcag acaatgggca ggattgtagg gaatttactg acgtaaaaaa
    31381 agagtatgac gattttgtta acaatttgtg caatcggcag catcgataag caggtcaaat
    31441 tctcccgtca ttatcacctc tgctacttaa atttcccgct ttataagctg attaaatgat
    31501 gaataaacgc ccctgttaat gaatatctgg catgttgtac taaaaatcga tgttttgctt
    31561 tgacaatccc ctggtgtttt gcgaaaacat tcgaggaaga aaaaaaacag tattcttata
    31621 tgcgcataac catgcatgta aataccatgt ttaccgtgct agtgaaatct acgtatggcg
    31681 tggacagacg ccattcgtga tgtcgatagc tgccacaagg caacggtctt ctcaccgtag
    31741 acccaggcat tgcgcgccgt gaatcttcat gatttcggtc tatcgtgacg ggtagcgact
    31801 ctgaacagtg atgtttcagg gtcagacagg agtagggaag gaatacagag agacaataat
    31861 aatggtagat agcaagaagc gccctggcaa agatctcgac cgtatcgatc gtaacattct
    31921 taatgagttg caaaaggatg ggcgtatttc taacgtcgag ctttctaaac gtgtgggact
    31981 ttccccaacg ccgtgccttg agcgtgtgcg tcggctggaa agacaagggt ttattcaggg
    32041 ctatacggcg ctgcttaacc cccattatct ggatgcatca cttctggtat tcgttgagat
    32101 tactctgaat cgtggcgcac cggatgtgtt tgaacaattc aataccgctg tacaaaaact
    32161 tgaagaaatt caggagtgtc atttagtatc tggtgatttc gactacctgt tgaaaacacg
    32221 cgtgccggat atgtcagctt accgtaagtt gctgggggaa accctgctgc gtctgcctgg
    32281 cgtcaatgac acacggacat acgttgttat ggaagaagtc aagcagagta atcgtctggt
    32341 tattaagacg cgctaacacg gaacaggtgc aaaatcggcg tattttgatt acactcctgt
    32401 taatccatac agcaacagta ctggggtaac ctggtactgt tgtccgtttt agcatcgggc
    32461 aggaaaagcc tgtaacctgg agagcctttc ttgagccagg aatacactga agacaaagaa
    32521 gtcacattga caaagttaag tagcggccgt cgccttctgg aagcgttgct gatccttatt
    32581 gtcctgtttg ccgtctggtt gatggctgcc ttactaagct ttaacccttc ggaccccagc
    32641 tggtcgcaaa cggcctggca tgaacctatc cataatttag gtgggatgcc cggtgcgtgg
    32701 ctggcagata cgctgttctt tatttttggc gtgatggctt acaccattcc cgtcattatt
    32761 gtcggcggtt gttggtttgc ctggcgtcat cagtccagtg acgaatacat tgattatttt
    32821 gccgtttcgt tacgcatcat tggcgttttg gcgctcatcc ttacctcctg tggtctggcg
    32881 gcaatcaacg ctgacgatat ctggtatttt gcctccggtg gcgtcattgg cagcttacta
    32941 agcactacgt tacaaccact gctacacagt agcgggggaa ctattgcgct gctctgcgtt
    33001 tgggctgcgg gcctgacgct gttcaccggt tggtcatggg tgaccattgc tgaaaaactc
    33061 ggcggctgga ttttaaacat tctcaccttc gccagtaatc gtacccgtcg cgatgatacc
    33121 tgggtcgatg aagatgaata tgaagatgac gaagagtatg aagatgaaaa ccacggcaaa
    33181 cagcatgaat cacgccgtgc ccgtattctt cgcggcgcgc tagcgcgtcg taaacggttg
    33241 gcggaaaaat tcattaatcc gatggggcgg caaacagacg ctgcgttgtt ctccggtaag
    33301 cggatggatg atgaagaaga gattatctac actgcacgcg gtgtggctgc cgacccggac
    33361 gacgtcctat tttcgggcaa tcgtgcaacg cagccagaat atgacgaata cgatccatta
    33421 ttaaacggtg cgccaattac cgaacctgtt gctgtagcag ctgctgctac cacggcgaca
    33481 caaagctggg ctgcgccggt tgaacctgtg acccagacgc cgcctgttac ttctgttgat
    33541 gttccacctt cgcaacctac agtagcctgg cagcctgtac cgggtccaca aacgggagag
    33601 ccggttattg ctcctgcacc ggaaggttac ccacagcagc cacaatatgc gcagcctgca
    33661 gtgcaatata atgagccgct gcaacaacct gtacagccac agcagccgta ttatgcgcct
    33721 gcagctgaac aacctgtgca acaaccttac tacgctcctg cggcggaaca acctgttcag
    33781 caaccgtatt acgcaactgc gccggaacag cctgcgcaac agccgtatta tgcccctgcg
    33841 ccagaacaac cggtggcagg taacgcctgg caagccgaag agcagcaatc cacttttgct
    33901 ccacagtcta cataccagac tgagcaaact tatcagcagc cagccgctca ggagccgttg
    33961 taccaacagc cgcaacccgt tgaacagcag cctgttgtgg agcctgaacc cgttgtagaa
    34021 gagacaaaac ccgcgcgtcc gccgctttac tattttgaag aagtggaaga gaagcgagcc
    34081 cgtgaacgtg aacaacttgc ggcctggtat caaccgattc cagaaccggt taaagaacca
    34141 gaaccgatca aatcttcgtt gaaagcgcct tctgctgcag cagtacctcc agtagaagcc
    34201 gctgccgctg tttctccgct ggcatctggc gtgaaaaaag cgacactggc gacgggggct
    34261 gccgcaaccg ttgccgcgcc agtcttcagt ctggcaaata gcggtggacc gcgtccgcaa
    34321 gtcaaagagg gaattggccc gcagttgcca cgaccgaaac gtattcgcgt gccaactcgt
    34381 cgtgaactgg cgtcttacgg tattaagctg ccctcacagc gtgcggcgga agagaaagcc
    34441 cgtgaagccc ggcgcaatca gtatgattct ggcgatcagt ataacgatga tgaaatcgat
    34501 gcgatgcagc aggatgaact ggcacgtcag ttcgcccaga cacagcagca acgctatggc
    34561 gaacagtatc aacatgatgt gcccgtaaac gcagaagatg cagatgctgc ggcagaggct
    34621 gaactggctc gtcagtttgc gcaaactcaa caacaacgtt attccggcga acaaccggct
    34681 ggggcgaatc cgttctcgct ggatgatttt gaattttcgc caatgaaagc gttgctggat
    34741 gatggtccac acgaaccgtt gtttacgcca attgttgaac ctgtacagca gccgcaacaa
    34801 ctggttgcac cgcagcagca gtatcagcag ccacaacagc cggttgcgcc acagcagcaa
    34861 tatcagcagc cgcaacagcc ggttgcgccg cagccgcagt atcagcagcc acaacagcag
    34921 gttgcgccgc agccacaata tcagcagccg caacaaccgg ttgcaccgca gcagcaggat
    34981 accctgcttc atccgctgtt gatgcgtaat ggcgacagcc gtccgttgca taaaccgacg
    35041 acgccgctgc cttctctgga tttgctgaca ccgccgccga gcgaagtgga gccggtagat
    35101 acctttgcgc ttgaacaaat ggctcgcctg gtggaagcgc gtctggctga tttccgtatt
    35161 aaagccgatg tcgtcaatta ctctccgggg ccggttatca ctcgctttga acttaacctg
    35221 gctcctggcg taaaagcggc gcgcatttct aacttgtcac gggaccttgc ccgttcactt
    35281 tcgacggtgg cggtgcgtgt cgttgaagtt attcctggca aaccctatgt aggtctggag
    35341 ttaccaaata aaaaacgaca aaccgtttat ctgcgcgaag ttttggataa cgccaaattc
    35401 cgcgataatc cgtcgccatt aaccgtggtg ctgggtaaag atatcgccgg tgagccggtg
    35461 gttgccgatc tggcgaaaat gccgcacttg ttggttgcgg ggactaccgg ttccggtaaa
    35521 tctgtcggtg tgaacgcgat gatcctgagc atgctttata aagcacagcc agaagatgtg
    35581 cgtttcatca tgatcgaccc gaaaatgctg gagctttcgg tttatgaagg cattccgcat
    35641 ctgttaacgg aagtcgttac tgatatgaaa gatgccgcca acgcgctgcg ctggtgtgtt
    35701 aacgagatgg agcgtcgtta taaactgatg tctgcgctgg gtgtgcgtaa tctggcgggt
    35761 tataacgaaa aaattgctga agccgatcgc atgatgcgtc cgattccaga cccgtactgg
    35821 aagccgggtg acagtatgga tgcccagcat ccggtgctga aaaaagaacc atacattgtg
    35881 gtgttggttg acgaatttgc cgacctgatg atgacggtag gtaaaaaagt ggaagagctg
    35941 atagcacgtc tggcgcaaaa agcccgtgcc gcgggtatcc acctcgtact ggcaactcag
    36001 cgtccatcgg ttgatgttat tactggtctg attaaagcga atattccgac ccgtatcgcc
    36061 tttaccgtat ccagtaagat tgactcacgt accattcttg atcaggctgg cgcggaatca
    36121 ctgctgggga tgggggatat gctctactct gggccgaact ccacgttgcc ggtacgtgtc
    36181 catggtgctt ttgttcgcga tcaggaagtt catgccgtgg tgcaggactg gaaagcgcgt
    36241 ggtcgcccac agtatgttga tggcatcact tccgacagtg aaagcgaagg tggcgcgggc
    36301 ggtttcgatg gcgctgaaga actggatccg ttgttcgatc aggcggtgca gtttgtcact
    36361 gaaaaacgca aagcgtcaat ttctggcgta cagcgtcagt tccgcattgg ttataaccgt
    36421 gcagcgcgta ttatcgaaca gatggaagcg caggggattg tcagcgaaca ggggcacaac
    36481 ggtaatcgtg aagtgctggc cccaccgccg tttgactaat taatgcattg ccggataagg
    36541 cgcggtagcg tcgcatccgg cactctatca actgaaaatt cagtattttc ttttttcctc
    36601 aagctgatta ttagcctgga atagagagta gagggaactc ccgatcggga gtgacgtaat
    36661 ttgaggaata atgatgaaaa aaattgccat cacctgtgca ttactctcaa gcttagtagc
    36721 aagcagcgtt tgggctgatg ccgcaagcga tctgaaaagc cgcctggata aagtcagcag
    36781 cttccacgcc agcttcacac aaaaagtgac tgacggtagc ggtgcggcgg tgcaggaagg
    36841 tcagggcgat ctgtgggtga aacgtccaaa cttattcaac tggcatatga cacaacctga
    36901 tgaaagcatt ctggtttctg acggtaaaac actgtggttc tataacccgt tcgttgagca
    36961 agctacggca acttggctga aagatgccac cggtaatacg ccgtttatgc tgattgcccg
    37021 caaccagtcc agcgactggc agcagtacaa tatcaaacag aatggcgatg actttgtcct
    37081 gacgccgaaa gccagcaatg gcaatctgaa gcagttcacc attaacgtgg gacgtgatgg
    37141 cacaatccat cagtttagcg cggtggagca ggacgatcag cgcagcagtt atcaactgaa
    37201 atcccagcaa aatggggctg tggatgcagc gaaatttacc ttcaccccgc cgcaaggcgt
    37261 cacggtagat gatcaacgta agtagaggca cctgagtgag caatctgtcg ctcgattttt
    37321 cggataatac ttttcaacct ctggccgcgc gtatgcggcc agaaaattta gcacagtata
    37381 tcggccagca acatttgctg gctgcgggga agccgttgcc gcgcgctatc gaagccgggc
    37441 atctgcattc tatgatcctc tgggggccac cgggtaccgg caaaacaact ctcgctgaag
    37501 tgattgcccg ctatgcgaac gctgatgtgg aacgtatttc tgccgtcacc tctggcgtga
    37561 aagagattcg cgaggcgatc gagcgcgccc ggcaaaaccg caatgcaggt cgccgcacta
    37621 ttctttttgt tgacgaagtt caccgtttca acaaaagcca gcaggatgca tttctgccac
    37681 atattgaaga cggcaccatc acttttattg gcgcaaccac tgaaaacccg tcgtttgagc
    37741 ttaattcggc actgctttcc cgtgcccgtg tctatctgtt gaaatccctg agtacagagg
    37801 atattgagca agtactaact caggcgatgg aagacaaaac ccgtggctat ggtggtcagg
    37861 atattgttct gccagatgaa acacgacgcg ccattgctga actggtgaat ggcgacgcgc
    37921 gccgggcgtt aaatacgctg gaaatgatgg cggatatggc cgaagtcgat gatagcggta
    37981 agcgggtcct gaagcctgaa ttactgaccg aaatcgcagg tgaacgtagt gcccgctttg
    38041 ataacaaagg tgatcgcttt tacgatctca tttccgcact gcataaatcg gtacgtggta
    38101 gcgcacccga tgcggcgctg tactggtatg cgcgaattat tactgccggt ggcgatccgt
    38161 tatatgttgc gcgtcgctgt ctggcgattg cgtctgaaga tgtgggtaat gctgatccac
    38221 gagcgatgca ggtggcaatt gcagcctggg attgctttac tcgcgttggc ccggcggaag
    38281 gtgaacgcgc cattgctcag gcgattgttt acctggcctg cgcgccaaaa agcaacgctg
    38341 tctacactgc gtttaaagct gcgctggcgg atgctcgcga acgtccggat tatgacgtgc
    38401 cggttcattt gcgtaatgcg ccgacgaaat taatgaagga aatgggctac gggcaggaat
    38461 atcgttacgc tcatgatgaa gcaaacgctt atgctgccgg tgaggtttac ttcccgcggg
    38521 aaatagcaca aacacgctat tatttcccga caaacagggg ccttgaaggc aagattggcg
    38581 aaaagctcgc ctggctggct gaacaggatc aaaatagccc cataaaacgc taccgttaat
    38641 gttatcgttg cggtaatgtt gttactgtat ccctgtggtc gcaggctgtg gccacatctc
    38701 ccatttaatt cgataagcac aggataagca tgctcgatcc caatctgctg cgtaatgagc
    38761 cagacgcagt cgctgaaaaa ctggcacgcc ggggctttaa gctggatgta gataagctgg
    38821 gcgctctcga agagcgtcgt aaagtattgc aggtcaaaac ggaaaacctg caagcggagc
    38881 gtaactcccg atcgaaatcc attggccagg cgaaagcgcg cggggaagat atcgagcctt
    38941 tacgtctgga agtgaacaaa ctgggcgaag agctggatgc agcaaaagcc gagctggatg
    39001 ctttacaggc tgaaattcgc gatatcgcgc tgaccatccc taacctgcct gcagatgaag
    39061 tgccggtagg taaagacgaa aatgacaacg ttgaagtcag ccgctggggt accccgcgtg
    39121 agtttgactt tgaagttcgt gaccacgtga cgctgggtga aatgcactct ggcctcgact
    39181 ttgcagctgc agttaagctg actggttccc gctttgtggt aatgaaaggg cagattgctc
    39241 gcatgcaccg cgcactgtcg cagtttatgc tggatctgca taccgaacag catggctaca
    39301 gtgagaacta tgttccgtac ctggttaacc aggacacgct gtacggtacg ggtcagctgc
    39361 cgaaatttgc tggcgatctg ttccatactc gtccgctgga agaagaagca gacaccagta
    39421 actatgcgct gatcccaacg gcagaagttc cgctgactaa cctggtgcgc ggtgaaatca
    39481 tcgatgaaga tgatctgcca attaagatga ccgcccacac cccatgcttc cgttctgaag
    39541 ccggttcata tggtcgtgac acccgtggtc tgatccgtat gcaccagttc gacaaagttg
    39601 aaatggtgca gatcgtgcgc ccggaagact caatggcggc gctggaagag atgactggtc
    39661 atgcagaaaa agtcctgcag ctgctgggcc tgccgtaccg taaaatcatc ctttgtaccg
    39721 gcgacatggg ctttggcgct tgcaaaacct atgacctgga agtatggatc ccggcacaga
    39781 acacctaccg tgagatctct tcctgctcca acgtttggga tttccaggca cgtcgtatgc
    39841 aggcacgttg ccgcagcaag tcggacaaga aaacccgtct ggttcatacc ctgaacggtt
    39901 ctggtctggc tgttggtcgt acgctggttg cagtaatgga aaactatcag caggctgatg
    39961 gtcgtattga agtaccagaa gttctgcgtc cgtatatgaa cggactggaa tatattggct
    40021 aatacccaat ttttctgaat ctaaaaagcg cctgcgggcg ctttttttgt ctccctttga
    40081 taccgaacaa taattactcc tcacttacac gtaatactac tttcgagtga aaatctacct
    40141 atctctttga ttttcaaatt attctatgta tacaagccta tatagcgagc tgctatagaa
    40201 ataattacac aatacggttt gttactggaa tcaatcgtga gcaagcttga gtgagccatt
    40261 atgaaaacga aaatccctga tgcggtattg gctgctgagg tgagtcgccg tggtttggta
    40321 aaaacgacag cgatcggcgg cctggcaatg gccagcagcg cattaacatt accttttagt
    40381 cggattgcgc acgctgtcga tagcgccatt ccaacaaaat cagacgaaaa ggttatctgg
    40441 agcgcctgta cagttaactg tggtagtcgc tgcccgctac gtatgcacgt cgtggacggt
    40501 gaaatcaaat atgtcgaaac ggacaatacc ggcgatgaca attacgacgg cctgcactag
    40561 gttcgcgcct gcctgcgtgg gcgttccatg cgtcgccgtg tctacaatcc ggaccgcctg
    40621 aaatatccga tgaaacgagt cggggcgcgc ggtgaaggca aattcgagcg cattagctgg
    40681 gaagaagcct acgacatcat cgcgaccaat atgcagcgcc tgatcaaaga gtacggcaac
    40741 gagtctatct atctgaacta tggcaccggt acgctgggcg gcaccatgac ccgctcctgg
    40801 ccgccgggaa ataccctggt cgcgcggctg atgaactgct gcggcggcta tctgaaccat
    40861 tacggcgact actcctccgc gcaaattgcg gaaggtttga actataccta cggcggctgg
    40921 gcagatggca acagcccgtc ggatatcgaa aacagtaagc tggtagtgct gtttggtaat
    40981 aaccctggcg aaacgcgaat gagtggcagt ggggtgactt actatcttga acaggcacgc
    41041 cagaaatcta atgcccgcat gatcatcatc gatccgcgct ataccgacac cggtgccggg
    41101 cgcgaagatg agtggatccc tattcgtccg ggaacagatg ccgcactggt taacggtctg
    41161 gcgtacgtca tgatcactga aaacctggtg gatcaggcat tcctcgataa atattgcgtt
    41221 ggctacgatg agaaaaccct gccagccagt gcgccgaaaa atggccacta taaagcttat
    41281 attctgggtg aagggccaga tggcgtggct aaaacaccgg aatgggcctc acaaatcacc
    41341 ggtgttccgg cagacaaaat catcaaactg gctcgtgaaa tcggcagtac caaaccggcg
    41401 tttatcagcc agggatgggg cccgcagcgt cacgctaacg gtgaaatcgc aacccgtgct
    41461 atctcgatgc tggcgattct gaccggtaac gttggtatta acggaggcaa cagcggcgcg
    41521 cgtgaaggtt catacagctt accgtttgtc cgtatgccga ccttggaaaa cccgatccag
    41581 accagcattt cgatgtttat gtggaccgat gccattgaac gtggcccgga aatgacggcg
    41641 ctgcgtgatg gtgtacgcgg gaaagataag ctggatgtgc cgatcaaaat gatctggaac
    41701 tatgccggta actgcctgat taaccagcat tctgaaatca accgtaccca tgaaatcctt
    41761 caggatgata agaagtgcga gctgattgtg gttatcgact gccacatgac ctcatcggcg
    41821 aaatatgctg acatcctgct gcctgactgc accgcttccg aacagatgga ctttgcgctg
    41881 gatgcatcct gcgggaatat gtcttacgtg attttcaacg atcaggtgat taaaccgcgc
    41941 tttgaatgta agaccatcta tgaaatgacc agcgaactgg caaaacgtct tggcgttgag
    42001 caacagttta ctgaaggccg tacacaggaa gagtggatgc ggcatctgta tgcccagtcg
    42061 cgggaagcga ttcctgaact accaacgttt gaagagttcc gcaagcaggg gatctttaaa
    42121 aagcgcgacc cacaagggca ccacgttgct tataaagctt tccgtgaaga tcctcaggca
    42181 aattcactga ccacgccatc gggcaaaatt gagatttatt cgcaggcgct ggctgacatt
    42241 gccgctacct gggaattgcc agaaggagat gtgatcgatc cactgccgat ctacacgccg
    42301 ggctttgaaa gttatcagga tccgttgaac aaacagtatc cgctgcagct tacgggtttc
    42361 cactataaat ctcgcgttca ctcaacttac ggcgacgttg atgtgctgaa agcagcttgc
    42421 cgtcaggaaa tgtggatcaa cccgcttgat gcgcaaaaac gcggtatcaa caacggcgat
    42481 aaagtcagga tctttaacga tcttggtgag gttcatattg aggcgaaagt gacgccacga
    42541 atgatgccgg gtgtggtcgc actgggtgaa ggtgcctggt atgacccgga tacaaaacgt
    42601 gtcgataagg gcggttgtat caacgtactg accactcaac gtccgtctcc tctcgctaag
    42661 gggaatccgt cacatacaaa ccttgttcag gttgaaaagg tgtaaggagt aaccgatgac
    42721 aacccagtat ggatttttta ttgattccag ccgttgcacc ggttgcaaaa cctgcgagct
    42781 ggcctgtaaa gactacaaag atttgacgcc agaagtcagc ttccgccgca tttatgaata
    42841 tgctggcggc gactggcagg aagataacgg tgtctggcac cagaacgtgt ttgcctacta
    42901 tctgtcgatt tcatgtaacc actgcgaaga tccggcttgt actaaagtct gcccgagcgg
    42961 tgcgatgcat aaacgtgaag atggttttgt tgtggtcgat gaagatgtgt gcattggctg
    43021 ccgctactgc catatggctt gcccgtatgg cg
//
LOCUS       DAZPEB010000010        42378 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZPEB010000010 DAZPEB010000000
VERSION     DAZPEB010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42378
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.010"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..86
                     /locus_tag="ACHURJ_000522"
     CDS             <1..86
                     /locus_tag="ACHURJ_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1279595.1"
                     /translation="TTGLAVVITTTYNETRIQPNTKCPEKS"
     gene            102..641
                     /locus_tag="ACHURJ_000523"
     CDS             102..641
                     /locus_tag="ACHURJ_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFR1279596.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQVAANKRAATAAANKKARQDKLDAEASADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(736..2313)
                     /gene="guaA"
                     /locus_tag="ACHURJ_000524"
     CDS             complement(736..2313)
                     /gene="guaA"
                     /locus_tag="ACHURJ_000524"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFR1279597.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMHMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYNKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2382..3848)
                     /gene="guaB"
                     /locus_tag="ACHURJ_000525"
     CDS             complement(2382..3848)
                     /gene="guaB"
                     /locus_tag="ACHURJ_000525"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFR1279598.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4010..5380
                     /gene="xseA"
                     /locus_tag="ACHURJ_000526"
     CDS             4010..5380
                     /gene="xseA"
                     /locus_tag="ACHURJ_000526"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFR1279599.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5377..5592)
                     /locus_tag="ACHURJ_000527"
     CDS             complement(5377..5592)
                     /locus_tag="ACHURJ_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFR1279600.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(5661..7133)
                     /gene="der"
                     /locus_tag="ACHURJ_000528"
     CDS             complement(5661..7133)
                     /gene="der"
                     /locus_tag="ACHURJ_000528"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFR1279601.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEVEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7251..8429)
                     /gene="bamB"
                     /locus_tag="ACHURJ_000529"
     CDS             complement(7251..8429)
                     /gene="bamB"
                     /locus_tag="ACHURJ_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFR1279602.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYD
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8440..9060)
                     /gene="yfgM"
                     /locus_tag="ACHURJ_000530"
     CDS             complement(8440..9060)
                     /gene="yfgM"
                     /locus_tag="ACHURJ_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFR1279603.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9078..10352)
                     /gene="hisS"
                     /locus_tag="ACHURJ_000531"
     CDS             complement(9078..10352)
                     /gene="hisS"
                     /locus_tag="ACHURJ_000531"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFR1279604.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDLNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPIVDIYLV
                     ASGADTQSAAMALAERLRDELQGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     TNGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10463..11581)
                     /gene="ispG"
                     /locus_tag="ACHURJ_000532"
                     /gene_synonym="gcpE"
     CDS             complement(10463..11581)
                     /gene="ispG"
                     /locus_tag="ACHURJ_000532"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFR1279605.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(11608..12621)
                     /gene="rodZ"
                     /locus_tag="ACHURJ_000533"
     CDS             complement(11608..12621)
                     /gene="rodZ"
                     /locus_tag="ACHURJ_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFR1279606.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLLGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTAATTPDGAAPLPTDQAGVTTPVADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(12906..14060)
                     /locus_tag="ACHURJ_000534"
     CDS             complement(12906..14060)
                     /locus_tag="ACHURJ_000534"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFR1279607.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14210..14641)
                     /gene="ndk"
                     /locus_tag="ACHURJ_000535"
     CDS             complement(14210..14641)
                     /gene="ndk"
                     /locus_tag="ACHURJ_000535"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFR1279608.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(14790..17102)
                     /gene="pbpC"
                     /locus_tag="ACHURJ_000536"
     CDS             complement(14790..17102)
                     /gene="pbpC"
                     /locus_tag="ACHURJ_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFR1279609.1"
                     /translation="MLRLLTKRGCWITLAAVPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKILQLWRALQLEWHLSKREILILY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLEWPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDCTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTSCP
                     PYGHYAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(17103..22064)
                     /gene="yfhM"
                     /locus_tag="ACHURJ_000537"
     CDS             complement(17103..22064)
                     /gene="yfhM"
                     /locus_tag="ACHURJ_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFR1279610.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPANALPGLRPQFALK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPSDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPLVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGIALKTMGDATRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFGERWLSTQESNALFLAAP
                     TIQDLPGKWQAQTSFSAEPLTGEKTLNSNLNSDQLATLQVRNSGDQPLWLRMDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGDEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            22264..23109
                     /gene="sseA"
                     /locus_tag="ACHURJ_000538"
     CDS             22264..23109
                     /gene="sseA"
                     /locus_tag="ACHURJ_000538"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFR1279611.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPKTFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            23445..23570
                     /gene="timP"
                     /locus_tag="ACHURJ_000539"
     CDS             23445..23570
                     /gene="timP"
                     /locus_tag="ACHURJ_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFR1279612.1"
                     /translation="MKIRCFCIVLIVSGALFSQVNNNRSLSGDNLLVVNNLQSSK"
     gene            complement(23602..24378)
                     /gene="sseB"
                     /locus_tag="ACHURJ_000540"
     CDS             complement(23602..24378)
                     /gene="sseB"
                     /locus_tag="ACHURJ_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFR1279613.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLFIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(24520..25803)
                     /gene="pepB"
                     /locus_tag="ACHURJ_000541"
     CDS             complement(24520..25803)
                     /gene="pepB"
                     /locus_tag="ACHURJ_000541"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFR1279614.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAAHKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRASERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(25981..26181)
                     /gene="iscX"
                     /locus_tag="ACHURJ_000542"
     CDS             complement(25981..26181)
                     /gene="iscX"
                     /locus_tag="ACHURJ_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFR1279615.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(26193..26528)
                     /gene="fdx"
                     /locus_tag="ACHURJ_000543"
     CDS             complement(26193..26528)
                     /gene="fdx"
                     /locus_tag="ACHURJ_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFR1279616.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(26530..28380)
                     /gene="hscA"
                     /locus_tag="ACHURJ_000544"
     CDS             complement(26530..28380)
                     /gene="hscA"
                     /locus_tag="ACHURJ_000544"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFR1279617.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(28397..28912)
                     /gene="hscB"
                     /locus_tag="ACHURJ_000545"
     CDS             complement(28397..28912)
                     /gene="hscB"
                     /locus_tag="ACHURJ_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFR1279618.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(29008..29331)
                     /gene="iscA"
                     /locus_tag="ACHURJ_000546"
     CDS             complement(29008..29331)
                     /gene="iscA"
                     /locus_tag="ACHURJ_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFR1279619.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(29348..29734)
                     /gene="iscU"
                     /locus_tag="ACHURJ_000547"
     CDS             complement(29348..29734)
                     /gene="iscU"
                     /locus_tag="ACHURJ_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFR1279620.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLVEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(29762..30976)
                     /gene="iscS"
                     /locus_tag="ACHURJ_000548"
     CDS             complement(29762..30976)
                     /gene="iscS"
                     /locus_tag="ACHURJ_000548"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFR1279621.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(31088..31576)
                     /gene="iscR"
                     /locus_tag="ACHURJ_000549"
     CDS             complement(31088..31576)
                     /gene="iscR"
                     /locus_tag="ACHURJ_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFR1279622.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(31846..32586)
                     /gene="trmJ"
                     /locus_tag="ACHURJ_000550"
     CDS             complement(31846..32586)
                     /gene="trmJ"
                     /locus_tag="ACHURJ_000550"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFR1279623.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            32705..33508
                     /gene="suhB"
                     /locus_tag="ACHURJ_000551"
     CDS             32705..33508
                     /gene="suhB"
                     /locus_tag="ACHURJ_000551"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFR1279624.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            33653..34507
                     /locus_tag="ACHURJ_000552"
     CDS             33653..34507
                     /locus_tag="ACHURJ_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR1279625.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQDDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKETKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            34698..35978
                     /gene="csiE"
                     /locus_tag="ACHURJ_000553"
     CDS             34698..35978
                     /gene="csiE"
                     /locus_tag="ACHURJ_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFR1279626.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(35970..37109)
                     /locus_tag="ACHURJ_000554"
     CDS             complement(35970..37109)
                     /locus_tag="ACHURJ_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFR1279627.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLAMMTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYQVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVLQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGAFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(37269..38159)
                     /gene="hcaR"
                     /locus_tag="ACHURJ_000555"
     CDS             complement(37269..38159)
                     /gene="hcaR"
                     /locus_tag="ACHURJ_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFR1279628.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKTVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            38295..39656
                     /gene="hcaE"
                     /locus_tag="ACHURJ_000556"
     CDS             38295..39656
                     /gene="hcaE"
                     /locus_tag="ACHURJ_000556"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFR1279629.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKSPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            39653..40171
                     /gene="hcaF"
                     /locus_tag="ACHURJ_000557"
     CDS             39653..40171
                     /gene="hcaF"
                     /locus_tag="ACHURJ_000557"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFR1279630.1"
                     /translation="MSAQVSLELHLRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYQAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            40171..40491
                     /locus_tag="ACHURJ_000558"
     CDS             40171..40491
                     /locus_tag="ACHURJ_000558"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFR1279631.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGSDIFIDLP
                     EAQP"
     gene            40488..41300
                     /gene="hcaB"
                     /locus_tag="ACHURJ_000559"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             40488..41300
                     /gene="hcaB"
                     /locus_tag="ACHURJ_000559"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFR1279632.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            41310..42327
                     /gene="hcaD"
                     /locus_tag="ACHURJ_000560"
                     /pseudo
     CDS             41310..42327
                     /gene="hcaD"
                     /locus_tag="ACHURJ_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
BASE COUNT        10020 a        11714 c        10639 g        10005 t
ORIGIN      
        1 ttacgacagg tttagccgtt gttattacca caacgtataa cgaaacgcgt attcagccaa
       61 atactaaatg tcctgaaaag agctaataat caggaggagt catgaagaaa gtttttcttt
      121 gcgccatctt ggcctcctta agctatccgg ctatcgcctc atcattgcag gatcaactct
      181 ctgctgtagc agaagcggaa cagcaaggta aaaatgaaga gcaaaggcag catgacgaat
      241 gggtcgcgga gcgcaacagg gaaatccagc aagagaagca acgtcgcgca aatgcccagg
      301 tcgccgctaa caaaagagcg gcaacggcag cagcaaataa gaaagctcgt caggataaac
      361 tggacgccga agcctctgcg gacaaaaaac gcgatcaaag ttatgaagat gagctacgca
      421 gcttagagat tcagaaacaa aaactggcgc tggcaaaaga agaagcccgc gttaagcgag
      481 aaaacgaatt tatcgatcag gaactgaagc acaaagctgc gcaaaccgat gtggtgcaat
      541 ctgaagctga cgccaacaga aatatgactg aaggcggtcg cgatctgatg aaaagcgtgg
      601 gcaaagcaga agagaacaaa tcggacagct ggtttaatta atcgatgtta gtaacttcaa
      661 gcctatgatt cttgaagata aaaaaccctc tgtagtaaca gagggttttg ttcattcata
      721 gtgcagggat taaaatcatt cccactcaat ggtagctggc ggcttgccgc tgatgtcata
      781 caccacgcgg gaaataccgt tcacttcatt gataatgcgg ttggaaacgc gaccgaggaa
      841 atcgtacggc agatgcgccc agtgtgcggt cataaagtcg atggtttcga cagcacgcag
      901 agagacaacc cagtcatact tacgaccatc gcccattacg ccaacggaac gtaccggcag
      961 gaacacagtg aacgcctggc tgactttgtt gtacaggtcc gctttacgca gttcttcaat
     1021 gaagatggcg tcagcacggc gcagcaggtc acagtactct ttcttcactt cacccagcac
     1081 acgaacgcca aggcctggtc ccgggaacgg gtgacggtac agcatgtcgt acggcaggcc
     1141 cagctccaga ccaatcttac gcacttcgtc tttgaacagc tctttcagcg gttcaaccag
     1201 gcccatcttc atctctttcg gcaggccgcc cacgttgtgg tgagatttga tgacgtgtgc
     1261 tttaccggtt gcagaagccg cagattcgat aacgtcaggg tagatggtgc cctgcgccag
     1321 ccacttcacg tcttccagtt tcagcgcttc ttcatcgaac acttcaacga aaacgcgccc
     1381 gatgatttta cgttttgctt ccggatcgtt ttcgccagcc agcgctgaca ggaagcgatc
     1441 ttctgccggt acgtgaacaa tgttcagacc aaagtgatcg ccaaacatat ccagaacctg
     1501 ctctgcttcg ttgagacgca gcaggccgtt gtcgacgaat acgcaagtca gatttttacc
     1561 gatagcgcgg tgcagcagca ttgcggttac ggaggaatcc acaccaccag agaggccgag
     1621 gatgacttta tcgtcgccta cctgctcgcg gatgcgagct acagcatcgt cgataatttt
     1681 cgctggcgtc cacagggctt cacactggca gatatcacgc acaaaacgct ccagcatgtg
     1741 cataccctgg cgggtgtgag tcacttccgg gtggaactgt acgccataga agcgtttttc
     1801 ttcgttagcc ataatggcaa acgggcagct ttcggtgctg gctacggtga cgaagtcgga
     1861 cgggatagcg gtaactttat cgccgtggct catccagaca tcgagcagcg gtttaccgtc
     1921 tgcggtcagc gcatcttcga taccgcgaac cagtgcgctg tcgtttacga cttcaacctg
     1981 cgcgtagcca aattcacgtt cgttagaggc ttcaacgtga ccgcccaact gcattgccat
     2041 ggtctgcatg ccatagcaaa cgccgaatac cggtacgcct gcttcaaaga catactgcgg
     2101 cgcacgcgga ctgttttctt cagtagtact ttccgggccg ccggaaagaa taatgccgct
     2161 tggattgaag tcacgaattt gtgcttctgt cacatcccac gcccacagtt cgcagtaaac
     2221 acccagctca cgcacgcggc gcgcaaccag ttgagtgtac tgagaaccga agtccagaat
     2281 gaggatgcga tgcttatgaa tgttttccgt cattgacgct tattccgagg caagtgaaac
     2341 agataatata aatcgcccga catgaagtcg ggcgaagaga atcaggagcc cagacggtag
     2401 ttcggggact ctttagtaat ggtcacgtcg tgaacgtggc tttcctgaat gcccgcaccg
     2461 ctgatacgta caaactccgc tttagtacgc agttcgtcga tagtaccaca gccggtcagg
     2521 cccatacagg agcgcaggcc gcccatctgc tggtgaatga tctctttcag gcgaccttta
     2581 taggctacgc gaccttcgat accttccggc actagtttgt cggcagcgtt gtcactctgg
     2641 aagtaacggt cagaagaacc tttggacatc gcacccagag agcccatacc acggtaagat
     2701 ttgtaagaac ggccctggta gagttcaatt tcacccggag attcttcagt acccgccagc
     2761 atggaaccca ccattaccgc gcttgcgcca gcggcgatag ctttggcgat gtcgccggag
     2821 aagcgaatac caccatctgc gataaccgga atacctgtgc cttccagcgc ttcaactgcg
     2881 tcagcaacgg cagtgatctg cggaacacct acgccagtaa cgatacgagt cgtacagata
     2941 gaaccagggc cgataccgac tttaaccgcg ctgcaaccag cttctgccaa agcgcgtgca
     3001 cctgcagctg ttgccacgtt gccgccgatg atttgcagat ccggatattt agcacgggtt
     3061 tcacggatac gctgcaaaac gccttctgag tggccgtggg aggagtcgat cagcagaacg
     3121 tcaacgccag cggcaaccag cgcgtcaaca cgctcttcgt tacccgcacc tgcgccaact
     3181 gcagcaccaa cacgcagacg gccttgctcg tctttacagg cgttcggttt acgttccgct
     3241 ttctggaagt ctttcacggt gatcatgccg atcaggtgga attcgtcatc aaccaccagc
     3301 gctttttcaa cgcgtttttc gtgcattttt gccagcacca cttcacgggc ttcaccttca
     3361 cgcacggtga ccagacgctc tttcggcgtc atgtaaacgc taaccggctg gttcaggtcg
     3421 gtaacaaaac gcacgtcacg accggtgata atacccacca gttcgttttc ttcggtaaca
     3481 accggatagc ccgcaaaacc gttacgctcg gtcagttctt tcacttcgcg cagcgtcgtg
     3541 gttggcagaa cagtctgcgg atcagtcacc acaccagatt cgtgtttttt cacacggcga
     3601 acttcttctg cctggcgttc aatggacatg tttttgtgga taaagccgat accgccttcc
     3661 tgagccagag caatagccag gcgcgcttcc gttacggtat ccattgctgc ggaaagcata
     3721 gggatattca gacgaatagt tttcgtcagc tgggtgctga ggtcagcagt attcggcaga
     3781 acggtagaat gagcaggaac gaggagaacg tcgtcaaacg tcagagcttc tttagcgata
     3841 cgtagcatgg gcaatatctc gaccagagtg gttaataaat attgccgcgg cattatacag
     3901 agcgtaaccg attgcatcta cccctttttg caaaaaatgc ttgctatccc cgaagggcgg
     3961 gttactatcg actgaataac ctgctgattt agaatttgat ctcgctcaca tgttaccttc
     4021 tcaatcccct gcaattttta ccgttagtcg cctgaatcaa acggttcgtc tgctgcttga
     4081 gcatgagatg ggacaggttt ggatcagcgg cgaaatctct aatttcacac aaccggcttc
     4141 cggtcactgg tactttacac tcaaagacga caccgcccag gtacgctgcg cgatgttccg
     4201 caacagcaac cgccgggtga ccttccgtcc acagcatgga caacaagttt tagttcgcgc
     4261 caatattacg ctctacgagc cgcgcggtga ctaccagata atcgttgaga gtatgcagcc
     4321 ggccggtgaa gggctgctgc aacagaagta cgaacagctc aaagcgaagt tgcaggctga
     4381 aggtttgttc gatcagcaat acaaaaaacc acttccctcc cctgcgcatt gcgttggtgt
     4441 gatcacctca aaaaccggtg ctgcgctaca tgatattttg catgtgttaa aacgtcgcga
     4501 tccttctcta ccggtgatca tctaccccac cgccgttcag ggcgatgacg caccggggca
     4561 aattgttcgc gccattgaac tggcgaatca gcgcaatgag tgcgacgtgt tgatcgttgg
     4621 gcgcggcggc ggttcgctgg aagatttatg gagttttaac gacgaacgcg tagcgcgggc
     4681 gatttttgcc agccgcattc cggtcgtcag cgccgtcggg catgagacgg atgtgaccat
     4741 tgccgatttt gttgccgatc tgcgtgcgcc aacgccgtct gccgccgctg aagtagtgag
     4801 ccgtaatcag caagagttac tgcgccaggt gcaatcgacc cgtcaacggc tggagatggc
     4861 gatggattat tatctcgcca accgcacgcg tcgctttacg cagatccatc accgattaca
     4921 gcaacagcat ccgcagctcc ggctggcacg ccagcaaacc atgcttgagc gcctgcaaaa
     4981 gcggatgagc tttgcgctgg aaaatcaact taagcgtacc gggcaacagc agcagcggtt
     5041 aacacagcgg ctgaatcagc aaaatccaca gccgaagatt catcgcgcgc aaacgcgcat
     5101 tcagcaactg gaatatcgtt tagcagaaac cctgcgcgta cagcttagcg ccacgcgtga
     5161 acgtttcggt aatgcagtaa cgcacctcga agccgtaagc ccactgtcaa cgctggcgcg
     5221 tggatacagc gttactactg ctactgacgg caatgtactg aaaaaagtga agcaagttaa
     5281 agcgggtgaa atgctaacca cacgtctgga agacggctgg atagaaagtg aagttaaaaa
     5341 catccagcca gtaaaaaaat cgcgtaaaaa ggtgcattaa gccagcacga attcaacgcg
     5401 ttttttcgat atcaagccat gcccgtgctg acagaagtaa tctactgcac cgcaggcttt
     5461 taacacctgc aacggttgat ggcagtctgg acaaagagct ttcatttcaa taatttcccc
     5521 gcaggatggg caacgcgcat ggccgttatc ctgatcaagc acatgctgac attgtggaca
     5581 atgcagttcc atatgaattc ctccggtagc catccctcta cattcataga gggaatggca
     5641 gataaaatac ttacggataa ttatttattt ttcttgatgt gcttcatcag acgcttacgt
     5701 ttacgcatct gggttggcgt cagggtgtta cgcttgttcg catacgggtt ttccccttct
     5761 ttgaactgaa tacgaatcgg cgatcccatt acgtccagcg atttgcggaa gtagttcatc
     5821 aagtagcgct tgtaggaatc aggcaggtct ttcacctgat taccgtgaat caccacaatc
     5881 ggcgggttat aaccaccagc gtgggcatat ttcagcttca cacgacgacc gcgtaccagc
     5941 ggcggttggt gatcttcaac agccatcgtc atgatgcgcg tcagcataga ggtccccaca
     6001 cgacgggtgg agctgtcata cgcttcacgt actgattcaa acaagttacc aacaccactg
     6061 ccgtgcaagg cagagataaa gtgcacacga gcaaaatcga taaagcccag acggaagtcc
     6121 agcgtttctt tcacctgctc tttcacttcc tggctcaggc catcccactt attcaccaca
     6181 atgacaagtg agcgcccact attgagaata aagcccagca gcgagagatc ctgatcggaa
     6241 ataccttcgc gcgcatcaat cactaacatc accacgttgg catcttcaat ggcctgcaac
     6301 gttttgatta cggagaattt ctctacagca tcggtgattt tgccgcgttt acgtacgcca
     6361 gcggtgtcaa tgagcacata ctcacgtcca tcgcgttcca ttggaatgta gatactgtca
     6421 cgcgtcgtgc caggcatgtc ataaacaaca acgcgctctt caccaagaat acggttagtg
     6481 agtgtagact tacctacgtt cggacgaccc acaatcgcca gtttgatcgg cagactttgc
     6541 gggtcgaagt cgtcttcctc ttcttcttcg ccgttctctt ccacttcaaa ttgcgcccag
     6601 tattcagcgt cttcgtcgac ttcctcttgc ggtgcgagat cttccatcca cggcagcagc
     6661 acatgctcca gcagacttaa tacgccacga ccgtgagacg cggcgatcgg gtagatttca
     6721 cctaagccaa gcgagtagaa atcaaccact gcctgatcgg gatccagacc gtcagttttg
     6781 tttgccacca ggaaggtcgg tttttcacgg gagcgcagat gtttggcaat cgcttcatct
     6841 gccggcatca ggcccgcgcg cgcatccacc ataaacagta cgacgtccgc ttcttcaatc
     6901 gccagcagcg actgttccgc catgcgggtt tctacaccgt cttctgtgcc atcaatcccg
     6961 ccggtatcaa tacagataaa ctcacggcct tcaatttccg cacgaccgta cttacggtca
     7021 cgagtcagac ccgggaaatc cgcaaccagc gcatctcggg tgcgagttag acggttaaat
     7081 aacgtggatt ttcctacgtt agggcgcccg acaagcgcga ccacaggtac catgtttaaa
     7141 gcctcatttt tataaatcat cagacaacgc acgctatatt cgcgtcgttg ttaaaaacag
     7201 gaaaacggcc cctgtccagg agccgttttc aaagtgaacg acagagacga ttaacgtgta
     7261 atagagtaca cggttccgtc ttttgcctgg atcagcagtt tgccgtcagc ggcaaccggt
     7321 tcagtctgga aaccggaact atcaactttt tgctgggcaa cgaaacgacc atcttcgaca
     7381 ttaatccagt gcagataacc ttcactgtca ccgaccacca ggttgccatc atacagcacc
     7441 ggagaagtca gcaggcgatg caacagatcg ctttgtgtcc acagcgtaac gccgccatca
     7501 atggtcaacg ccatcacccg gtcattttga tcgaccagat agatgcgatt gccgtcgacg
     7561 atgaaatcat tcaccgaacc cagttcgcgt ttccacataa tctgaccact gcgcagatca
     7621 agcgccgtca ggttaccatt ataggccagc gcgaaaacaa cgccgttaac aacgacggga
     7681 gtcgtgtcaa catcgctcag acggtcaatt tcggtagaac cggtcgcctg ggaaatacgc
     7741 tgctgccaaa tcatctggcc ctgttccatc agcactgcgc tgacgcgacc attatcgccc
     7801 cccacgacgg ccgcaccaaa agccgttgcc ggcgcagact cgccacgcaa agagagcgaa
     7861 ggcatatcga ggttaactgt ccatttgaca gcgccgtcag cttcgttcag cgcttgtaac
     7921 tgaccgttac tggtgtggat taacaccaga ccgtcgctga ccaccgggcg cgaaagtgct
     7981 tcacccgcga ctttagtttg ccatgccaca gtaccatcgc tggtattcag cgcgtaaacc
     8041 tgcgcctttt cgctgccaat gtagacatgc ccaccagaca cggtcacacc gccagaaagt
     8101 aatgcaggct ctttagagaa ccagccatct ttctcggcca ggctgacaga ccagatttct
     8161 ttgccatcat ccgcattcag cgcttttacc aaaccagcgc ggtccgctgc atagacaacg
     8221 ttgtccgcca gtgccggatg aagattggaa tagaagttgc caatgccgct accaacggaa
     8281 gtactccacg ccgtggtcgg cgtaaactgg ttttcaacgg ttggcaatgg ggacatcttt
     8341 accacatctt cttcgctgtt aaacagcgaa cagccgctta aaagggtaac ggaaagcagt
     8401 cctggcagca gtaatttacg caattgcatc gggtccctct cagatggaca aattattaat
     8461 tttcatctgc atcatttcgc tcagtgccgg agtaacatcg cttttcacgc ctgcttccca
     8521 tgcactacgc gcaccttgct tatcaccttt gctcagcaat gcttcaccac gcaggtcggc
     8581 aacaatggca gcccaccctt cacctttgat ggtatcaagg gttttcagcg cggcatcagc
     8641 ctgcttgagc tgtacctgaa cgcgagcaag acgcagattt atcacggctt tgagattttc
     8701 atcgctcgtg tctgccagcc cctgttgtaa ctgggcggca gctttctcta gttcattttt
     8761 gtcaacaaat tgctgcgcaa gttccaaaga agccagcgca ccataagtat ttttattttc
     8821 agcagcaaat ttttccgccg ccgggatgct atccggtttg ccttcgctca ctgcggtaac
     8881 cgcattttga taggcaagag aagcggagcg tgcagaatca acctgatggc tgttccagta
     8941 gcgccagcca atcagtgcgc caacgcccaa aatcacccca acagccagtg ctttgccatt
     9001 ttcagcaaaa aagcgtttaa ccgcttctac ctggtcgttt tcgttctcgt aaatttccac
     9061 gctgtccttc tccttcctta acccagtaac gtgcgcaaat gcgcggctac gctatcctgc
     9121 gcaactgccg tttgctcacc agagcgcaaa tccttcacta ctgctgtgcc gttagtcact
     9181 tcagactcac ccagcaccac agcaacgcgg gcaccccatt tatcagcacg ggcaaactgt
     9241 ttcttaaagt tgccgccgcc gtggttggtc atcaatttca cgccctgtaa ttcatcacgc
     9301 agacgctcag ctaatgccat agccgcagat tgtgtatcag cacctgaagc caccaggtat
     9361 atatcgacaa taggatcggc tttaaattcc ggattaacgg cctgtactaa caatacaaga
     9421 cgttcgaggc ccatcgcaaa accgacagcc ggtgttgcac gaccgcccag ttgttccaca
     9481 agaccgtcat aacgaccgcc tgcacacacg gtgccctggg agccgagact gttagtcacc
     9541 cactcgaaaa cggtacggtt atagtaatcc agaccacgca ccagacgctg gtttacggtg
     9601 taagcgatcc ccgcgctctc aagcagtttg cacagaccgg caaaatgctc acgagattcc
     9661 tcgtccagat aatcacctaa tgccggagcg tcgttgagaa gcgcctgcac ttccggattt
     9721 tttgaatcca gcacgcgcag cgggttagtg tacatgcggc gtttgcagtc ttcgtccagc
     9781 ttttctttat gctgctcaag gaatgccacc agcgcatcgc ggtaattggc gcgtgcttcc
     9841 agcgaaccga tagaattcag ctcaagagtt acgtgttcgg aaatacccag cgcgcgccac
     9901 cagcgggcgg tgagcataat cagttcagcg tcgatatccg gaccttgcag accgaaaact
     9961 tcgcagccca actgatggaa ctgacgataa cgccctttct gcggacgctc gtgacggaac
    10021 atcggcccga tataccacag acgctgttcc tgattgtaca gaagaccatg ctcgatgccg
    10081 gcgcgtacac agcccgccgt cccttcaggg cgcagagtca ggctgtcgcc attgagatcc
    10141 tcaaaggtgt acatctcttt ttcaaccacg tcggtgactt caccaatcgc acgtttgaat
    10201 agcggggtct gctctacaat cggcaagcgg atttcactgt aaccgtagct gccgagcacg
    10261 tttttcagtg tgccttcaat gcgctgccag atggccgttt cgccaggcag gtaatcgttc
    10321 atgccgcgaa tggcttgaat gttttttgcc acgtttattc tctttctgaa tataaaaatg
    10381 aaccctcaac gcttccctca atgtttgggg agccatgcgg gttcaatcat acacgggaag
    10441 cgaggcgctt cccatcacgt tattattttt caacctgctg aacgtcaatt cgacgcgctt
    10501 catccagctg actggctttc gcacgaatgc gtgcttccag ctggtcgatc atatcgttgt
    10561 tgtccagacg gtctttgcgc acgccatctt catagaggcc gcttttcttg ttgccgccgg
    10621 tgacgccgag tgtagaaacc agcgcctcac ctgggccatt caccacgcag ccgataatag
    10681 aaacgtccat cggagtgatg atatcttcca ggcgttgctc cagcgcgtta accgtaccga
    10741 taacatcaaa ttcctgacgc gaacaggtcg ggcaggcgat gaagttgatc cctcgcgaac
    10801 ggatacgcag cgatttcaaa atatcgaaac cgactttgat ctcttcgacc ggatcggccg
    10861 ccagcgatac gcgcagcgtg tcgccgatgc cttcagacag cagcagacct aaaccaatgg
    10921 cggattttac tgccccgctg cgtgcaccac cggcttcggt gatccccaga tgcagcggct
    10981 gatcgatctg ttttgccagc aaacgataag actcaacagc gaggaagacg tcagacgctt
    11041 tcacgctgac tttgaactga tcgaagttca ggcgatcgag atgatcaaca tgacgcatgg
    11101 cggattccag caacgcctgc ggcgtcggtt cgccatactt ttcttgcaga tctttttcca
    11161 gcgatccggc gttaacgcca atacggatcg gaatgttttt atcgcgcgca cagtcaacca
    11221 ccatgcgaat acgctcttca ttaccgatat tgccagggtt aatacgcaga caatcgacgc
    11281 cgtattccgc tactttcagc gcaatgcgat agtcgaagtg gatgtcagcc accagcggca
    11341 cgttaacccg ctgtttgatg agtttgaacg cttctgccgc gtccatcgtc ggtacggaga
    11401 cacggacgat atcagcgcca acgcgttcca gcgccttgat ttgattgacc gttgcttcga
    11461 catctgtcgt acgcgtattg gtcatggact gtacggcgat gggagcacca tcgccaatcg
    11521 gcacattccc aacgtaaata cgtgttgatt ttctacgttg aattggagcc tggttatgca
    11581 tgaaaaatct cccgcgttac ccgtctgtta ctgcgccggt gattgttcgg cattgagggt
    11641 cagacgcgca acctggttag ttctgataaa acgactcaga tcgacaggtt tcccttgata
    11701 ctggatctgt actgcggctg gcgcaccaat tttcagtttg tacggtgcct ggccggttaa
    11761 gttcaaatta ccgtctttac gctgcatacc gctaaacaat tttttaccgg tagcatcagt
    11821 gacctccagc cagcaatcgg cagtaaagtt catcaccagc gcattcggat cagccaccgg
    11881 cgtggtcacg ccagcctgat cggttggcaa cggcgcagca ccatctggcg ttgttgctgc
    11941 cgttggtgcc ggggtcgcgg cggtatcaac atttgcctgc gaaggcgaaa caaccgcatt
    12001 ctgttgcgga tcaacagctg gtgctggcgc agttacggca ggtgtttgcg tgttggttgc
    12061 ggtagtatcc acagacgctg gcggcgtgga cgttgtagcc gggtctgtag ttgtcgacgt
    12121 atttaacgga acactctgcc cctgctcgct attactgctc agttccgccg aagattgatc
    12181 ggccatagtg gtgatctctt cctgctgagc tttgtggtct tgccaccacc aggcaccgct
    12241 caggccgata accacaaaca acaccagcca ggtgaaggtc atcagccagc cgtcacgttt
    12301 tttgcggcgt ttaccgaggg aaaaactctg catcggcgca actttcgcag cccgtagtgg
    12361 agcctgcttt tccagcccta gcagcagttc ttcttctgga atatgtacca gacgcgcata
    12421 agagcggata tatccgcgca ggaatgttga agcaagatcg gcgggtgcct tatcttcttc
    12481 aatgtcgcgt accgtggaaa ccttcaggca aagtcgctcg gcaacggcct gctgactaag
    12541 tcctagttgt tcgcgagcat tacgcaggcg agcgccggta gtaagtgctt cattttggtc
    12601 gtgcgtggct tcagtattca ttcgctgcag gtacgtttaa atgagaattg agatgccggt
    12661 gaaccatcat gctcacccac accgcgaaac atccgttaag ttaaccgtta tcatacagta
    12721 taagaccgtc tgtgtgctcg tgacaaagcc cacattcact aaggctaact tactgttgca
    12781 tcgttacata ctgccttaaa gtcagcaaaa acgcaccgtt aatattgacc agacaattgc
    12841 aacttaatac ttcattcata aaagattacg ccacggtaca taaagtaacc gtggcgtaat
    12901 ggctatcaga ccgctttaat gtcgatggct tcaccctgca tccgtttacg cagggtacgt
    12961 ttcgtacggt cgataacatc gcccgccaac tgaccacagg cagcatcgat atcatcacca
    13021 cgagttttac gcacaatagt ggtgaaaccg tagctcatca gcacttttga gaaacggtcg
    13081 atacggctgt tcgaactgcg tccatacggc gcgcccggga acgggttcca cgggatcagg
    13141 ttgatcttac acggcgtatc tttcagcagc tccgccagtt ggtgcgcgtg ttcagtgccg
    13201 tcgttaacgt ggtcaagcat cacgtattca atagtgactc ggccctgatt ggcgttggat
    13261 ttctccagat aacggcgcac cgcagcaagg aacgtttcga tattgtactt tttgttgatc
    13321 ggcacaattt cgtcacgaat ttcgtcgttc ggcgcgtgca gggaaattgc cagtgcaacg
    13381 tcgatcatat cgcccagttt atccagcgcc ggaactacac cggaagtaga aagcgtgacg
    13441 cgacgtttag acaggccaaa accgaaatca tccagcatga tttccatcgc cggaacgacg
    13501 ttgttcaggt tgagcagcgg ctcgcccatg cccatcatca ctacgttagt gattgggcgc
    13561 tgaccggtga cttttgctgc gccgacgatt ttcgccgcac gccacacctg gccgataatt
    13621 tccgacaccc gcaggttgcg attaaagccc tgctgggcgg tggaacagaa tttacactcc
    13681 agcgcacacc ccacctgcga agagacgcag agcgtggcac ggtcgtcttc cgggatatac
    13741 accgtttcga cgcgctgatc gccaacggcg atcgcccatt taatggtgcc gtcggatgaa
    13801 cgctgttctt caaccacttc cggtgcgcgg atttccgcca cctctttcag tttgccgcgc
    13861 aacactttgt tgatgtcggt catctcatca aagttgtcgc agcaatagtg atacatccac
    13921 ttcatcacct gatcggcgcg gaagggtttt tcacctaaat ctttaaaaaa ctcccgcatc
    13981 tgctgacggt tgagatccag caggttgatt tttccatctt tcgtggtgac gttttcaggt
    14041 gtgactaatt gttcagacat atgctattcc ggcctcgtta ttacacgtta tggcccctgg
    14101 agggttgaaa aaagaaacgc cccggtgagc ggctgctcgt ccgggggcgc tgcattgtac
    14161 aaattctggc gcacggatgc cacgtttgca cgcggcattt acgaaattat taacgggtgc
    14221 gcgggcacac ttcgccttcg ccaaagaaat aagcgatttc gcgagcggca gattcgacgg
    14281 aatcagaacc gtgggtaccg ttttcggtca ggctgtcagc gtaatcagcg cgcagagtac
    14341 cagccagtgc gtttgccgga ttggtcgcgc ccagcagatc gcggtgacgc tgaacggcgt
    14401 tttcaccttc cagcacggaa accacgatcg ggccagaggt catgaattca accagaccat
    14461 caaagaacgg ttttccatcg tgttcagcat aaaagccacg tgcctgttca acggtcaggt
    14521 gcagcatttt ggtgccgaca attttgaacc ctgcagcttc aaagcgcgca aagatattac
    14581 caatgacgtt ttttgctacc gcgttcggtt tgatgatgga aaaagtacgt tcaatagcca
    14641 tttttacctc tgtaaattgt tctgttgttg tctgtaccag cgtacgagat ggcgcggatt
    14701 ataatgagca acagggccgt tgactattga tgaaggtaac atttttttaa aataaaatga
    14761 gttttagcaa caatcaggga caaaacagac tattgcatga caaatttcac tgtcgcgatt
    14821 tgccccacgt catccatcac cagcaattga taatcacctt tatccgtcaa atgcagggtc
    14881 acgttgcgcc cgcgttcagt taacggttcg ccattcagaa accaccagcg ttcacctgcc
    14941 cctccacttg attgcaacgg caaagtcgct tccgctgcgc ccggtaaacg tttaataatc
    15001 gcgccatcgc gaacgcctgt cagctgcagc gggagctggg catagtgacc gtatggcggg
    15061 caacttgtcg aggccggtgg taagcgcaca gcgcgacgtt ctgatgcggg cagccagggt
    15121 tccagcggta gcggccagac atttatcatt tcttgtcgcg cttgcgggca atcagcggca
    15181 acacgtttgc cattttcatc cagccagatg gggaaacgaa tgccattaat gccttcctgc
    15241 tccggcagta atagagttgg cggctgactc ccgtccagca gccaggttgc caggcggcgg
    15301 cgacagttac cgtcaccttc cggcaaagac tgtccgcccg gccagcagat aacgccacga
    15361 gtgactgagt tcgggcgcgg gtcttccggc agattcgcac tgcgcgacag taagatatta
    15421 ttgacctgat tcaacaatgg tacggcactg gcaaagccaa actgaccaac aacgggcgtg
    15481 cagtccggtc tgccagtcca gatcccaatg acatagcgag cgttaacccc aatcgcccag
    15541 gcgtcacgat aaccatagct ggtgcccgtt ttccatgcca gtggggcgac gcgcggcaag
    15601 gcactatccg gcaagggttg cgcttcatca gccataatcc ggcgaatgat ccacgccgcc
    15661 cccgacgaca ttaaaggcca ttcaagcagc ggatcgtcag gctgtaagcg caatttgcct
    15721 gccttgccgt ggcgagcaaa cgcggtatac gctgccgcca tatcttccag ttttgcacca
    15781 gcgccgccga gaatgagtga aagattcggc gcagcaccgt tgggcaaata taacggcaat
    15841 ccaacattgc gtaactttgc cgcaaaccgt ttcggtccat aggcttccag cacctgcaca
    15901 gcaggtaagt tcagcgagcg caccagcgcc tcgctcatgc tgatcgggcc atgaaaaccg
    15961 ctatcaaagt tacctggtcg ataatcaccg gtgcgccggg ggacgtcttg cagcagtgat
    16021 gccgggtgga tcaagccttc atccagcgcc agaccataaa caaacggttt gagcaccgat
    16081 cctggcgatc ggatcgcatt gaccatatca acatgaccaa agcgtgaatc atcgttgaga
    16141 tcaaccgatc ccacccagcc gcgaacacgc atatcggtat gatcaaccac gatcatcgcc
    16201 agtgagctgc gcggtggcaa ccgccctttc cagttttgcg ccagttcttc cagacgtcgt
    16261 tgaagaccgg catccagcgt agtaacgatt ttgtcgcttt tgcttttacc gagcatcatg
    16321 cgcgaaaaca gcggtgccag ttgcggcatt tgtcggggtg ccagccagat gggttcttcc
    16381 cttgactctt ttacctgctc acgggaccac acaccttgca cggccatccg ttcgagcact
    16441 ttattacgcg cggcttcggc acgctccggc caacgatccg ggcgcagtcg gctgggcgct
    16501 tgcggtaaca ccgccagcat tgccgcctcg gaatagctta aattcgcagg cgattttccg
    16561 agataagccc aacttgccgc accgatcccc tgcaacgtac cgccaaacgg agcgcggtta
    16621 agatacaaga tcagaatttc acgcttagac aaatgccact ccagttgcaa cgcgcgccag
    16681 agctggagaa ttttgccacc aaatgttttg gggtgaggat caagcagacg agcaacctgc
    16741 atagtgagcg tgctgccacc agaaataacc cgtcccgaag tgagatcttg ccatgctgcg
    16801 cgcgccaccg agaatggatt cacccccgga tgcttccaga accagcgatc ttcataattg
    16861 atcagcgctt caaggtaacg tggagacaca tcttcgattg ttaccggata acgccagatg
    16921 ccatcagcat cggcgaagcg ccagagcggc gtaccatcct gcgccacgac cactcgtgcg
    16981 ggattgactt catgcagcgg tagaggccag agtttatctg ctccccacgc tgcaagaaaa
    17041 aggagaaatg gcacagctgc cagcgttatc cagcagccgc gtttggttaa caagcgaagc
    17101 atttacggtc ggacaatcag cagatcttca gccgcgccgg tcgcccgcca ttggggaaca
    17161 tacattgatt ccaccatcgg ttgcggtacc tgatacgttc ccggcgtcac cgcccgcgcc
    17221 agatacacca gcgttaccgg ttggtattca tcaacggcaa ccgccgccac aaagcgatcg
    17281 tcacggaact caatgtgctt aatgctcgcc tgctgcatct ggttcagtaa gttttgcact
    17341 tcgtcaccac tttgctccag gctggcgcta ccgttcgcca gattctgatt ttccagttcc
    17401 agacccgcag gcagcagatc cacgactaac gcatccggca cgctgttact ggcttttacc
    17461 tgcaaccaga ccagcaccag atcgccgcta cgtaacgagt ccagcgattt gctcttacca
    17521 tcagtaccaa gaatatgacg ctcgatttgc agcacattgt tcgccggtaa aggtgcggat
    17581 tgcggataac cgctggcatc catacgcaac cataacggct gatcgccact gtttctcacc
    17641 tgcaaggtgg caagttgatc gctattcaga ttgctgttta gcgttttctc gcctgtcagc
    17701 ggctcagctg agaaagaggt ttgcgcctgc catttacccg gtaaatcctg aatcgtaggg
    17761 gcagccagga acaacgcgtt actttcctgc gtcgatagcc agcgttcacc aaacgcctgc
    17821 tgcgaaagtg tgttcagcaa agtgtactgc tcatcgggta gcagtttatt ttcttccagc
    17881 aaggagagca ttaacgcgtt gtcgcgcagt ggactaccgt aatcacccag ccatatccgc
    17941 tcatcactat ttctcggcgt tttcagcgcc agcacaatcg cttcttcacc acgcgttgca
    18001 tcacccatgg ttttcagcgc aatgccaagt tgcagcagcg gtaaaccaga agcggcatct
    18061 gcacgatgct cccagatttc acgcagcgca cccagtggtg ctttttgctg acgggccagc
    18121 accagcgcag cgtaagactg aacggcgaat ttactggctt tgagattatc cgcgtacggg
    18181 atcgacatca tgcccggatc ttgtaaatag cgcagcagac gctcattacc ccggttaatg
    18241 gcgtctgtcg gcacgctgta accctgttcg cctgcgcgga ccaggaaatc catcacgtaa
    18301 gccgtcagcc agtactcttc gtcaccgttt ttatcccaca gcgcaaagcc gccgttatca
    18361 cgttgcattt gcagcaaacg ggaaatgccg atatcgaccg atgcacggcg tttctcatca
    18421 ctgtcgcctt tgatgcccaa cgcctgcagt tgggctgcgt tggtataaag tgacgggaac
    18481 aggccgctgg cggtttgctc aagacagccg tacggatacg cttttaactc tttgatataa
    18541 cgtgcaatgt tcagcggtgg tttgccgctc aacaacaatt gcccttccag cgtaacaggc
    18601 gagaagtttt gcaatccatc cgccggaatc gcccatgtct caccaggctg taacgccgta
    18661 ccgtaattaa ccgtttgtgc cgggaacgcc ggacggacgc cgattttcca ctgcttatgc
    18721 tgatcggcaa cggtttcacc cggtaacgct aacccgctaa tggtggcctg aatttctcca
    18781 tcgccataac ccggcaatgc tcgcaccggg ataaacagcg tagtacgcac acctggcgct
    18841 aattcaacgg ctgcgggtga atcgctgacc agttcaagca aaccactggc ggtcagggca
    18901 acgttcagtt tttgcggttt atcggtaaga ttagtgatat ccagcgtcag acgcgaggta
    18961 tcgccactcg ccataaagcg cggcatgttc agttcagcaa tcaccggtgc ggcaactatc
    19021 actttgcttt cgttgctacc gaaatcatct gccgtccagg cttgcgccat gacgcgcaat
    19081 tcaccgttaa aatcgccaat cggcagtgta accgagcctt cgccctgttc gttgagcgtt
    19141 accggcagcg cctgctgcgc gacaatattg acgtgattga ccagcggttt accaccacgt
    19201 ttcagctcat caccatcgcc accgaaacgc agagctgcca gacgcccctg accttcaata
    19261 acctgaccgt aaatatcgta aatgtctgcg ccatagcgtt tctgaccaaa gaacgcctgc
    19321 cacggatctg gcgtgacgta gtcagtaata ttcagcacac cactatcaac ggcagacacc
    19381 agcacattca cctgtttagg cttctcgcca tttttagtgc tggctttaat tttcacggtt
    19441 aatggttgat tgggacgcat ttttgctggt gtttccagcg ccagatcgag gcgacggttt
    19501 tcatcgccaa gcggcagatg caacacacca accgcgcgtt ttggcgtcgc ggagcgagat
    19561 ttatcgctag gacgtaccac cagcgtactt aaatacagat catgacgatt ccaggtttta
    19621 tcgaccggaa tcgtcagatc cagcccttga gccggaacat caatctcttg ccaccacagc
    19681 ggcccttcac tggactcgac catcgcataa cctttacccg ccgttggcgc ggcgatatgc
    19741 aacttaatgg tgtcgccagg gcgataactg gctttatcca gtttcagcgt gacacggtcg
    19801 ggtcgcactg cgccgctacc gtcgctgttg tcctgccagc tatagccagc ccagaaacga
    19861 acactactga ccgcttcatt cggcgctttg acttccagac gataagcgcc ccactctacc
    19921 ggaaaactga ccttgccggt ttcgtccgct ttcagatcca gagtttgttc attttcgatc
    19981 agatcttttt gatcaaactg tgactgccag ccttcatctt ctgaccagtt ccagtagtaa
    20041 tcgcggcgtt cgcgaatcag gcgcacctgc aagcccgaca cggctttttt cacgccttgt
    20101 gcatcgctat aaacgatgtc aaaagcggcg ttactgcctt catcaacaat gggctgtttc
    20161 accgtgctgt cagtgcgata atcgtaaacc gatttcaagg cgaactgcgg acggagcccc
    20221 ggcaatgcat tggcaggcca gatagcctgc tcagcgcggc gcgtcaccgg gcgaccgccc
    20281 gattccagca aactgccctg gaaaataacc tgcaatgggg aatgcgtttc cttccactgg
    20341 ctttctgtag aaacttcgcc gcgcccttta tcatccagcg tcaactgaac ttcatccagc
    20401 gtgcgggaaa ggttttcggc agctatatcg ccgaattcaa aaccaggtaa ggctgacaca
    20461 gcctcacgca gtgggcgcag gaaaagttgc ccttgcaaag tattaccatt agcaggcgca
    20521 ccgtacaggt agtatcccac cacggagaat ttcacttcat cattcggcgt aagcggggtt
    20581 ttctcaccgg tcagattcag cgccatacgc tctggcataa aatcttcgac gtggaaatcc
    20641 cacatccggt actggttatc gcccgtgttg gcgcgaatat gccacatacc ggttgccgca
    20701 ttgctatcga gtggccaggt aaagtggtag aggccattct ccggctgact aacgacgctc
    20761 ctgagtacct gcccgtcagg tttaatcacg tctaacttga tgggttgatc gggcaatgct
    20821 ttaccgtctg catcgcgcag taaaccattg aggattaccg tttcacctgg gcgataaaga
    20881 tcgcgtgggc caaacatgaa aaactgtttg ctatagcctg gcgcgccagc aatgttaaat
    20941 tctgctaagt ccagcgccgg aagttttaaa tcgagtagcg ttgtctgacc gtctttacgt
    21001 gccagcagta atgccgcatt tttatcattt tccagctgca catgcccctg agcgtcactg
    21061 gttgcctgag tcagagtctg ccctttctca tttaataaag agacttcaat tccttgctgg
    21121 gccgcgccgt tttccagact ttgggtaaag atatccagac gattgtgata acggtgagct
    21181 gaaacgccga tatcacttaa cgtaaacagc gtcgcgggat tactgtaatc gtaacgtcca
    21241 gcctgattca tcacagccag atacacgccc gcctgctgaa gcggtttgat atcgcccagc
    21301 ggcagcaata atttttcacg ggtgttacgc gcaggattga gatcaaaccg tccggtgtag
    21361 accagatccg ccatctgcag cagtttgtct gactgccagt tcgccagcga attgcggtat
    21421 tcccattggc taatgaatgc tggcagagat tctggcttaa cgcggaagaa gttaacatcg
    21481 acattattaa cgttgagcgc cattaccggc agcccttcaa cgactttgcc aggcagcagc
    21541 gaaccacggc tggcaaaacc gacgctgggt tggatgtcgc gggtagttat agttttttcg
    21601 taatctttac tgaaggttgc gttgttgagt gctttgacct ccttgccaat agtaacgatc
    21661 aaatcacgtt tcggttcgag gtggcgtaaa cgtagctctt taagattatc tgacagctcc
    21721 caggcaccat ccactttgcc gcttttttta tcgacgacat gaataacgcg tgagaaatcc
    21781 tgatccgggt cgagagggat ggagaacgtc agcaccagcg tggctgcacc atcaagttgg
    21841 acttcagaga gatccagcaa tgtcagcacc ttaccggcac tctgttgggc cagtttttgt
    21901 ctctccggca cggagagttt tgctgaactc gcgttttctg aagaggccgc tttagtaact
    21961 tcagaaggcg catctttttt caccgctgtt ggcgcgttat cgttgttgtc gcaccctgcc
    22021 agcgccagca ttagcatgca ggcggctacg cgtaactttt tcatccctgc cacaatggcc
    22081 cgttagcaac gtcgaataat tattatgcgt gagaatttac gttatgtaat tcagtatcac
    22141 gcccatacaa tccgaatttt agtgagaatt gtggctcaga cagccaaatc agcctctttc
    22201 gcttgtcgct tttcactaaa catccgacaa tttagcctaa ccccggcaaa aatggagatg
    22261 cctatgtcca cgacatggtt tgtaggagcc gactggctcg ccgaacatat tgatgacccg
    22321 gaaattcaga ttatcgatgc ccgcatggcg tcgcctggac aggaagatcg taacgttgct
    22381 caggagtatc tgaatggaca tattcccggc gcagtgtttt ttgatatcga agcgctttct
    22441 gatcacactt ccccgcttcc gcacatgctg ccgcgcccga aaacgttcgc cgtggcgatg
    22501 cgtgaattag gcgttaacca ggataagcac ctgattgtct atgacgaagg taatcttttc
    22561 tcagccccac gagcatggtg gatgctgcgc acctttggtg tagagaaagt gtcgattctg
    22621 gggggtggac ttgcaggctg gcagcgcgat gatctgctgt tagaagaagg tgcagtagag
    22681 ctgccggaag gagagtttaa cgccgcgttt aatcctgaag ccgtggtgaa agtaaccgat
    22741 gtattattgg caagccatga aaatacggcg caaattattg atgcccgccc ggctgcacgt
    22801 tttaacgcag aagttgatga acctcgccca ggtttacgtc gcggacatat tcccggagca
    22861 ctgaatgttc cgtggacgga actggtgcgc gaaggcgaac taaaaacgac cgatgaactg
    22921 gatgcgatat tttttggtcg cggcgtcagc tacgacaaac caattatcgt cagctgcggc
    22981 tctggtgtaa cggcagccgt ggttttgtta gcactcgcga cgctggatgt gccaaacgtg
    23041 aaactgtacg acggcgcatg gagtgaatgg ggcgcgcggg cagatttacc ggttgagcca
    23101 gtgaaataag tattttacag gcaataaaaa accgccgaat ttggcggttt tttattgctg
    23161 gtctggttcg cggcctttcc agcaggttgt attaccgtag taatgcaagc gcgtctcagc
    23221 ggagacaata ctcgccagta actctctttt tgtcaagcaa aagagagtta ttattgttct
    23281 gttagtatat tatccactgc ggccctttcc gccgtctcgc aagcgggcgg tggcttaagg
    23341 aaaggatgtt ccgcgaccgt tagagcaagc gcgtctcagc gcttgctatc gcggcaatat
    23401 cgccagtggt gctgtcgtga tgcggtcttc gcatggaccg cacaatgaag atacggtgct
    23461 tctgtatcgt acttattgtt tctggtgcgc tgttttccca agtaaataat aaccggagtc
    23521 tctccggcga caatttactg gtggttaaca accttcagag cagcaagtaa gcccgaatgc
    23581 cgccctttag ggcggcatat tttagattat ccggttttgt ttaaagtcac gcaaaaaacc
    23641 tccccagcga cgttcataga atggcacaat atgttcggta ataaagtggc taattccttt
    23701 ttcccctttt ttcacctgac aaatatcgat tggttcatcg ccaggtaatg tatcggtcgc
    23761 tacacttccc gtcgcctgaa taatttcttc gatatcacca tcggcttcaa tgccaataaa
    23821 taaattaggc tgtgcctctt cgttctcttt aattgaacaa ataaaagcac gcttcaccgg
    23881 cttaatggtt ttaaataagg tggtgagtga atcaatcatt tgtgctggcg gctctgcaac
    23941 ttccgataat atcagcgatt caccgccttc caggatttcc tggctgctca gcggatttcc
    24001 ctcttcacca atcaacaaac tgatttcacg cggcataaat tctttaccgg tcggcagttt
    24061 ggcattaagg aagagcgttt cgccaagggt catctcaaac aatgtgcgaa cgggcattac
    24121 gacaaatgcc tgttcgtctt caaccgcctg ttgaagcgct tctaacgagg tgaaaaaagg
    24181 aatgacgctg gtgccgtctt ctttttccca gtgctgtaaa tcaagcgcac tatcttcaac
    24241 cacagcctcg ccctgcgccg ccgtaccagg cacccagacg gtggattcca gtagagtacg
    24301 gaaaaaggcc gggcggtgtg ccggttcggt tgctgctttt tccagcaggt cttcaagttc
    24361 gttttttgtt tcggacataa gaaccacaat tcattcaacg ttcggggcgc aaatgcctga
    24421 tgcgctacgc ttatcaggcc tacaagggat tcgcaatttg ttgaatttgc agaatttgta
    24481 ggccggataa ggcgtttacg ccgcatccgg catattagtt tacgccgtta acagattagc
    24541 tatcgtgcgc acaccaagtc ccgtagcgcc cgcagaccac tgttcaaccg gcgctttacg
    24601 gtaagtcgcc gagcagtcga tatgcagcca gccttgctga tagttctcaa caaagtgcga
    24661 caggaagccc gccgccgtgc tcgcgcctgc cggatacgcc gcgcttccgg tattgttcag
    24721 ttcggcaaag ttagacggca gctggctgcg gtggaattcc gccagcggca gacgccagaa
    24781 tggttcgttc tcttgtgagg cactcgccag caaacgaccg gcaagcgcat cgtcaaaact
    24841 gaacagcgcg tgataatcat tacccagcgc agttttcgcc gccccggtga gggtcgccgc
    24901 atcaatgatc atttccggtt tctgcgcact ggcatcaatc agaccatcgg caagcaccag
    24961 acgcccttcg gcatcagtgt tcatcacttc aactttttta ccgttgcgat aggtgatgat
    25021 atcgcccagc ttgaacgcat tgccgctaat caggttatcc gcacagcaga ggaacagctt
    25081 cacgcgcttg ttcagtccgc gcgtaatggc aaatgccagc gccccggtaa ccgttgccgc
    25141 gccgcccatg tccgacttca tcgagtccat aaacgcagtc tgtttgatgc tgtagccgcc
    25201 ggagtcaaaa gtgatacctt tacctaccag gcacgcgtac actggcgctt ctttatcgcc
    25261 agttgggttg taatccagcg ccagcaatac cggagaacgt tctgaagcgc gtccgacggt
    25321 gtgcagcccc atataacctt gctcacgcag atcttcgcct ttggtgatcc gataagtcac
    25381 acgatcgccc gcgacgttgc tgatcagatc aacagcacgc tgtgccagtt gcgatggtcc
    25441 caattcttct gccggtgcgt tgatggtgtc acgcacccag tcgatgatca tcaggcggtt
    25501 atccagttcc tggcgctggg catcgtccag atccggccac tccactttac gcgtgccttt
    25561 cggggctttg taaccttgcc agaatgccca gcagcgatcc gcatcccagc cttcaccgct
    25621 taactgaaca tgcttgatgc ccagaccgtc aatcttgtgc gccgcacgct ggatcagccc
    25681 cagatcgtct gccccgttca aatgcagggt aatgccgtca ttattaatgc tgtaagttgc
    25741 tttttctccc cagcgcgcgt cggcaggttg ggtagagagg gtaatcttca tcgcttctgt
    25801 cattttagtt atccttcttt gtaaaagggc cgccagttgg cagccccagg atttttccgc
    25861 taaatgattc gcgttgcagg aaggcggcaa gtgagtgaag ccccaggagc atagataact
    25921 atgtgactgg ggtgaacgag cgcagccaac gcatctgcgg cgtgaagcat gacgcggaaa
    25981 ttattcggcc tcgtccagcc agactaacaa aatcgcttcg aggattttct cgttggatgc
    26041 ctgcgggtcg tcgtcgaaat cttccagatc gcaaatccac tgatgcatat cggtgaatcg
    26101 aaccgttttc ggatccagat caggatacgc atcgtacagt gcttcgccaa tttcgcggct
    26161 atcggtccac ttaagtccca tactaacctc tgttaatgct cacgcgcatg gttgatagtg
    26221 taacgcggga tttcgactac taaatcttcg tcggtaacgc gtgcctggca gcttaaacgg
    26281 ctttcgggct ccagtcccca ggctttgtcc agcatgtcgt cttcctgctc tgagctttct
    26341 ggcagcgagt caaaaccttc acgaacgatg cagtggcagg tggtgcaagc acaggatttt
    26401 tcacaggcgt gttcaatctc gataccgtta cgcagcgctg cgtcgagaat ggtttcaccg
    26461 ctattagctt ccagaacagc gccatcaggg cagaggtcct gatgaggcaa aataacaatc
    26521 tttggcatat taaacctcgt ccacggaatg gcctttcagc gcacgacgaa ccgactggtc
    26581 catgcggcga gcggcgaaat cctgggtttg tttgtctacg tttttaatcg cttgttcgat
    26641 ggcgtcaaca tcatcgccct gagccacttc actcaggtga gcggcagcat tgtcaatgac
    26701 ctgacgctct gcggcgctta acagcgcggc atcagcagcc agcgcgccgt gcagactttc
    26761 cagcacacgc gccgcttcta ctttttgttc tgccagcata cgggctttta cgtcctgctc
    26821 ggcatagctc attgaatctt tgatcatcga agcgatttcg ctatcggtca gaccgtaaga
    26881 aggtttgacc tgaatagacg cctcaacgcc ggtggatttc tccatcgccg tcacgctcaa
    26941 aagaccgtcg gcatcgacct ggaacgtcac gcgaatatgc gcaccgccag ccggtagcgc
    27001 cggaatacca cgcagcgcaa aacgcgccag tgagcggcag tcctgcacca gttcgcgctc
    27061 accctgcatt acatggatag acatcgccgt ctgaccatct ttaaaggtgg tgaaatcctg
    27121 agcgcgggcc accggaatag tggtattacg cggaatcact ttctccacca ggccgcccat
    27181 cgtttcgagg cccagcgaca gtgggatcac atcaagcagc agcatttcgc tgtccggctt
    27241 gttacccacc agaatatccg cctgaatcgc cgcgccaata gcgacgactt tatccgggtc
    27301 gatggaagtc agcggtggac gaccgaaaaa ttcgcctacc cgttcacgca ccagcggcac
    27361 gcgagtagaa ccgccaacca tcaccacttc cagcacttca tcagcttcta cgcccgcgtc
    27421 tttcagcgca cgacgacaag ccagtaaggt tcgttttacc agtggcgcga tcagttcatt
    27481 gaattgttca cggctgattt cgccctgcca gcccgcaacg ttaacggtca cggagtccgc
    27541 atcgctcagc gcgattttgg ctgcaatggc ggcatccagc agttcacgct gaacgcggtt
    27601 atcgctacga tcaggaatgt ccgcctgctc gcgaatgtaa tccgccagca gatggtcgaa
    27661 atcatcgccg ccgagcgcgg aatcaccgcc ggttgccagc acttcaaaca cgccgcgact
    27721 taagcgcaga atggaaatat caaacgtccc gccaccgagg tcataaacgg cgatcacgcc
    27781 ttcctgaccg gaatccagcc cgtaggcgat agccgcagcg gtcggttcgt taagtaagcg
    27841 cagaacatgc aaccccgcca gacgcgccgc gtctttggtg ccctgacgct gggcatcgtc
    27901 aaagtacgcc ggaacggtga taacgacacc atccagctcg cctgccaggg cttcagttgc
    27961 ccgcgccgcc agtgctttga ggatgtccgc agaaacgcgc accgggttca gcagccccgc
    28021 cgccgtttca atcattggca ggccgttttc gctggcctgg aattgataag gcaaatgcgg
    28081 atagcgttgc tggatatcag ccagcgagcg tcccatcagg cgtttaacag aactaatggt
    28141 gttggcggta tcgagcgctg cgttagtacg cgcgtcataa cccaccgaat gcccttgctg
    28201 ttgatagtga acaacagatg gcagcaggtg acggccttca tggtcggcta acgtttcggc
    28261 ctgaccgctg cgcactgtcg ctaccagcga gttggttgtg cccaggtcaa taccggccgc
    28321 cagacgacgc tgatgcggcg cagcactcaa accaggttca ctaatttgta ataaggccat
    28381 gtttagcttc cagaaattaa aaatcgagca gtttttcttc gagttgttcg gcactgcttc
    28441 gcagtttatc gagaaaacgc agcttacgca cggtatccgc cgccgcgtcc cacgtctcgt
    28501 tgtctaactg ttcaaccatc aactgatggc gggtatcaaa catctttttc acacgtttga
    28561 taaagctttc cagccgcgct tcatctttcg cctgttcgat ctcgtccagt tcttcgcgca
    28621 gctccagctg ttccatcagg aacgcggtgt cgcgcacagt atgctgctcg ctggcgagat
    28681 caaaaccgtg caaagaaagc aaatattccg cgcgcattaa cggatgacgc agcgtttgcc
    28741 aggcctggtt aatggttgca gattgctgta cggcggcgag ttgttccgcc tggcttccgc
    28801 tggcgaattt atcaggatga tactgacgtt gtagatcctg aaaacgcagg ctcagtgcct
    28861 gggtatcgag ttgataacgg gcaggcaagc caaagagggt gaagtaatcc ataacattct
    28921 cagggctgcg atccgccgcg ataacggcga atcaggtaaa acaaacccca cgcgcaggcg
    28981 accacggtgg ggttatcggt atgcgcatca aacgtggaag ctttcgccgc aaccacactc
    29041 atctttaacg ttcgggttgg tgaatttaaa cccttcattc aggccttctt ttacgaagtc
    29101 cagctgcgta ccgtccagaa attgcaggct tttgccatcg accacgactt tcacgccttt
    29161 gtcttcaaac acgatgtctt ccggcgtcgg ttcgtcaaca aattccagta cataagccat
    29221 acctgaacat ccggaggttc tcacgcccag acgcaggcca aaccctttgc cgcggttagc
    29281 cagaaaggta tttactcgcg ctgctgcact gtcgctcagt gtaatcgaca taaccaaacc
    29341 tcaactctta ttttgcttca cgtttgcttt tatagtccgc aatggcggct ttgatcgcgt
    29401 cttctgccag aatagaacag tgaattttca ccggcggcag ttcaagttct tcagcaatat
    29461 cggtgttttt gatcgcctgc gcttcgacga gagacttccc tttcacccat tcggtgacca
    29521 gggagctgga agcgatagca gaaccgcagc cgtaagtttt aaaacgcgcg tcttcaatga
    29581 taccttcatc gttgacttta atctgcaact tcatcacgtc gccacaggcc ggtgcaccca
    29641 ccatgccgct gccgacgttc tcgtcgttgt tgtcaaagga acccacgtta cgcggattct
    29701 cgtaatggtc gataactttt tcgctgtaag ccattataaa ttctcctgat tccgataccg
    29761 attaatgatg agcccattcg atgctgttca gatccacgcc ctgcttgtac atttcccaca
    29821 gcggagaaag gtcacgcaga cgaccgatgg atttacgaac taactcgatg gtgtagtcga
    29881 tctcttcttc agtagtaaaa cgacctaaag agaaacggat agagctatgt gccagctcgt
    29941 cgttcagccc cagcgcgcgc agcacgtagg acggttcgag gcttgctgac gtacaggcgg
    30001 aacctgaaga aactgcgagg tctttcagcg ccataatcag cgactcacct tcaacgtagt
    30061 tgaagctgac gttgagaatg ttcggcgcac cgtgttccag gtcaccgttc aggtaaactt
    30121 cttcgatatc tttgatgccg ttccacagac ggttacgcag gccgcgcaga cgttccatct
    30181 cggtcgccat ctcttctttt gcgatgcgat aggcctcgcc cattccgacg atctggtgaa
    30241 caggcagagt gccggaacgc ataccgcgct cgtgaccgcc gccgtgcatt tgcgcttcga
    30301 tgcgtacgcg cggtttacga cgtacataca gcgcaccgat acctttcggg ccatagattt
    30361 tgtgaccgga gaaagacatc aggtcaactt tcaactggct caggtcgata ggcagtttgc
    30421 ccacgctctg ggttgcatca acgtgataga taatgccacg agcacggcac atttcgccga
    30481 tagccgcgat atcctgcacc acgccgattt cgttatttac gtgcatgatg gaaacaagga
    30541 tggtgtcgtc acgcatcgct gcttcaagtt ctttcaggtc gataatgccg ttacgctgcg
    30601 gtgccaggta ggtgacttca aaaccttcgc gctccagctg acggcaggta tccagtaccg
    30661 ctttgtgttc ggttttgcta gtgatgatgt gcttgccttt tttctgataa aagttggctg
    30721 cacctttgat cgccaggttg tcagattcgg ttgcaccaga ggtaaagacg atttcacgcg
    30781 gatcagcgcc gaccagatcg gctatctgat tacgggcgat atctaccgct tcttcagcct
    30841 gccagccgaa acggtgagaa cgagaggccg ggttaccaaa ggttccgtcc atcgtcatga
    30901 actgcatcat tttctcggca acacgcgggt ccaccggcgt ggttgcggag tagtcgagat
    30961 aaatcggtaa tttcattgct ctataaactc cgtacatcac tcaatgcaag gaatcaggct
    31021 accggctgga tgtacgaccg tgtttacgga gtatttagca ctccggcctg attctgaatt
    31081 ctttttatta agcgcgtaac ttaacgtcga tcgcgtcttg tgtgcgggtg cgtggcgcgt
    31141 cgtgagtatg ctgacgacca gacacatcca gcacttcctg gttattaacc agttcgccta
    31201 aagtaatgtt gttgagaaaa ccggtgagac ggtcgctcaa atcacgccac agcgcgtggg
    31261 tcaggcattt atcgccgccc tggcagccgc ctttaccctg acaacgggtg gcatctacag
    31321 attcgtcaac ggcgctaatc acttcgccaa cggcgatgct gctggcatct ttgcctaaca
    31381 gataaccacc gcctggtcca cgtacgctgg aaaccagacc atttttacgc agacgggaaa
    31441 acagttgttc cagataagaa agggaaatgc cctgacgttc ggaaatatca gccaacggta
    31501 ccgggcccgc ttcagagttg agcgcaacgt caagcattgc ggtcacggca tagcgccctt
    31561 tagatgtcag tctcatgtct tacttcacct caaactcgcc cctgcccggg gttttttatt
    31621 gtaaaagtgg gggtattgaa tagcagggtc aagtctgaca ttcccgagta aattggtcaa
    31681 ctatttactt gactgattta gtcgggtatt taaccttcag tgccattttt ttatcgtggc
    31741 gtttgtacct gttgtcggat gcggtgtgaa caccttatcc gacctacggt tctgtacatt
    31801 gtaggcctga taagacgcgt taagcgtcgc atcaggcaac tactgttatt cggccttgtt
    31861 acctttattc tgctgctcaa tagaagccag aatcccgcgc aggatattca attcctggct
    31921 ttccgggcgc gcacgggtaa acagacggcg caatttattc atcacctgcc ccggatggtt
    31981 ttcacggata aaaccggttg ccagcagcgt ttgttccaga tgaccataaa aacgctccag
    32041 atcatcgacc agcggatacg gcgtctcttc atgctcgacc tgctcgccgt tttcctgagt
    32101 tgccagccag gccatgcgca cttcataggc aataacctga accgccatcg ccaggttcag
    32161 cgagctgtat tccgggttag ccgcaatcgc gacatgataa tggcatttct gcaactcttc
    32221 attggtcaag ccgacgcgct cgcgaccaaa caccagcgcc accggggtat ttgccgcttc
    32281 agcgacgctt ttcaggccgc attcgcgcgg gtcgagcatc ggccatggca gcgtgcggga
    32341 acgtgcgctg gtgcccacca ccaagctaca accggctaac gcttcgtcga gcgtatcgac
    32401 gatgtgagca ttaccaatca catcgctggc ccctgctgcc agggcaatcg cctgggagtc
    32461 gggtttcacc agtggattaa ccagccacag attggttaat cccattgttt tcatggcacg
    32521 ggcaacagaa cccatattgc cggtgtgtga cgtctccacc agcacaattc gaatattttg
    32581 cagcattgtc tttcttcatc taaagattat tcacgcatct tatcataaaa cgaagacaga
    32641 tgccgatctc gctgctatac tctgcgccgt tttcccgttc tttaacatcc agtgagagag
    32701 accgatgcat ccgatgctga acatcgccgt gcgcgcagcg cgcaaggcgg gtaatttaat
    32761 tgccaaaaac tatgaaaccc cggacgctgt agaagcgagc cagaaaggca gtaacgattt
    32821 cgtgaccaac gtagataaag ctgccgaagc ggtgattatc gacacgattc gtaaatctta
    32881 cccacagcac accatcatca ccgaagaaag cggtgaactt gaaggtactg atcaggatgt
    32941 tcaatgggtt atcgatccac tggatggtac taccaacttt atcaaacgtc tgccgcactt
    33001 cgcggtatct atcgccgttc gtatcaaagg ccgcaccgaa gttgctgtgg tatacgatcc
    33061 tatgcgtaac gaactgttca ccgccactcg cggtcagggc gcacagctga acggctaccg
    33121 tctgcgcggt agcaccgctc gcgatctcga cggtaccatt ctggcgaccg gcttcccgtt
    33181 caaagcaaaa cagtacgcca ctacctacat caacatcgtc ggcaaactgt tcaacgaatg
    33241 tgcagacttc cgtcgtaccg gttctgcggc gctggatctg gcttacgtcg ctgcgggtcg
    33301 tgttgacggt ttctttgaaa tcggtctgcg tccgtgggat ttcgcggcag gcgagctgct
    33361 ggttcgtgaa gcgggcggca tcgtcagcga cttcaccggt ggtcataact atatgctgac
    33421 cggtaacatc gttgctggta acccgcgtgt tgtgaaagcc atgctggcga acatgcgtga
    33481 tgagttaagc gacgctctga agcgttaatg actcaggcgg gtgatgtcac tcacccgccc
    33541 tcgcctttca ggcgctattc cgaaatactt cctcaccgct ttactttctt tcgtcactct
    33601 cccaccatct ttccccgatt aatggatgat aaagaagtca caaggaaagg ttatggcgct
    33661 gccagtgaac aaacgcgttc tcaaaattct gtttattctc tttgttgttg ccttctgcgt
    33721 ttatttagtg ccgcgcgttg ccatcaactt cttctattat cctgacgata aaatttacgg
    33781 tcccgacccc tggtcggcgg aatccgtcga atttacggct aaggacggta ctcgtctgca
    33841 aggctggttt attccttctt cgacgggccc tgctgacaat gccatcgcaa ccatcattca
    33901 tgctcacggc aatgccggaa atatgtccgc ccactggccg ctggtcagtt ggttacccga
    33961 gcgtaatttc aacgttttta tgtttgatta tcgcgggttt ggtaaatcaa aaggcacgcc
    34021 gtcccaggcc ggattgctgg acgatacgca aagtgccatc aatgtggtgc gccatcgcag
    34081 tgatgtaaac ccacaacggc tggtgctgtt cgggcagagt attggcgggg cgaatattct
    34141 tgctgttatt ggtcaggatg atcgtgaagg catacgtgcg gtgatcctcg actccacatt
    34201 tgcctcttat gcaaccatcg ccaatcagat gatccccggc agtggctact tacttgatga
    34261 gagttacagc ggcgaaaatt atatcgccag cgtcagcccg atcccgcttt tactcattca
    34321 cggtaaagct gatcacgtta tcccatggca gcacagcgaa aagttgtata gcctggcaaa
    34381 agagacaaaa cggttgatcc taatcccgga tggcgaacac attgatgctt tttccgatcg
    34441 tcacggcgat gtttatcgcg aacagatggt ggactttatc cttagcgcgt tgaatccgca
    34501 gaactaaccc atgatcgcta gcacgataat cattcacaaa accaccttaa gacatgctaa
    34561 tccactggtc agaacagttt aagatgagaa aaattctgtg acgcttgcca acatttctga
    34621 tgattagcat tcccttcgcc atttacctga gcaaacttta gctattctta tcaattatgc
    34681 ttatgggaga tctacagatg atgcctacgc ttgctccacc atctgtcctt tcggctcccc
    34741 agcgccgctg ccagatcttg ctgacgctct ttcagccggg gttgaccgcc accatggcaa
    34801 ccttcagcga gcttaatggt gtggatgatg atattgccag tcttgatatc agcgaaacag
    34861 gacgggagat cctgcgctat catcaactca cactgacgac tggttatgac ggtagctacc
    34921 gggttgaagg tacagtgctt aaccaacgtt tgtgtttatt tcactggcta cgacgtggtt
    34981 tccgtctgtg tccgtcattt attaccagcc aattcacccc cgccctgaag agtgaactga
    35041 agcggcgcgg aattgcgcgt aacttttacg acgataccaa tctacaagcg ttagtgaatc
    35101 tctgctcccg acggctgcaa aaacgctttg aaacgcgcga tattcatttc ctgtgtctgt
    35161 atctgcaata ttgtttgctg caacaccacg ctggaattac gcctcagttt aatccgctcc
    35221 aacgtcgctg ggcggaatcc tgccttgaat ttcaggtagc gcaggaaatt ggacgccact
    35281 ggcagcgtcg ggcgctccag cctgtaccgc ctgatgagcc actgtttatg gcattacttt
    35341 tttccatgtt gcgggttccc gatccattgc gtgatgcgca tcagcgagac agacaattgc
    35401 gtcagtctat caaacgtctg gtaaaccatt ttcgtgagct gggaaatgtt cgtttttatg
    35461 atgaacaggg gttatgcgat cagctttata cccacctcgc ccaggcgtta aatcgcagtt
    35521 tgtttgccat cggtattgat aataccctgc cagaagagtt cgccagactg tacccacgcc
    35581 tggtgcgcac cacccgcgcg gcgctggccg gatttgaaag tgaatacggc gtccatcttt
    35641 ctgatgagga aagtggtctg gtcgcggtga ttttcggtgc ctggctaatg caggaaaacg
    35701 atctgcatga aaaacagatt attcttctta ccgggaatga tagcgagcga gaagcgcaga
    35761 ttgagcagca gttacgcgaa ctaacgttac tgccgctcaa cattaaacat atgtcggtaa
    35821 aggcattttt gcagacaggc gctccgcgcg gcgcggcact gattattgcg ccttatacca
    35881 tgccgttacc gctcttttca ccaccgctga tctatacgga cctgacactg acaacacatc
    35941 aacaggagca gatccgcaaa atgctcgaat cagcatgagg gaacaacttt cgggcgcaaa
    36001 aacattgccg gaagcgccac cagcgccatt acccagaaca cgccgtggcc cagatattga
    36061 tacaggaaac cggcgaaaac ggtcatgata gctatactgc cgcccatcgc gacggcagag
    36121 taaaccgcct gtaaacggat gacttcgcta ccctggcgag cggcaatata gcgcatggcg
    36181 gccaggtggc agaccgtgaa ggcgccgcaa tgcagaattt gcaccactat caaccacggc
    36241 aacgccgtag ttgctcccat aatgccccag cgcactacgc cgcaaatcgc cgagatcaac
    36301 agcatatcgc gtgcactaca acggcggaaa agtttattac tcagcgcaaa gataatgact
    36361 tccgccacca cgcccagcga ccacaaatac cccaccgccg aggccgagta gccagctgcc
    36421 tgccagtaaa tggcgctaaa accgtaatag gccgcatgtg ccccctgcaa taaacaaacg
    36481 caggccagaa agcgccagtt ctgaagaacc agcgccaacc acgctgacca accggtgctc
    36541 tcctgctggc ggcttgcccc ttgtggctga atcgtcggac ggatgagaaa gccgagcagc
    36601 atagatgcca cgcccaatgt taacagcgcg aggatcacct gataatcaaa catagtgacc
    36661 agtttgcccg tcagcgccga gccaatgaca aacgccaccg agccccacag tcgcactttg
    36721 ccgtaatcaa gcgggaactg cttttgccac gtattcgcca gtgcatcggt caacggtacc
    36781 agcggtgaga aaaagaggtt aaagccaatc atcaccagca tcagccacgc tacgtgcgcc
    36841 cccgcccaga aggcgacagc aaagagaagt gtcatcattg ccagcacgcg caaggcggaa
    36901 atcaggcgag aaggatcgct gacgcggggc gcgatgagca aactcccgag aaaacgggca
    36961 accagacctg cccccaataa caggccgatg gtttctggcg ttaaaccaat ccctttaagc
    37021 cagacgctcc agaaaggtag aaaaatgccg taactaaaaa agtatgtgaa atagccgagc
    37081 gccaaccagc gcgtggattg caaaaccatg agtccctccc gtcaggaggc gttagtctgg
    37141 ggttaatcga atgttggtgc aagttgaaaa ataagaatat tcgagcctgc ttcccgatgt
    37201 ctgtaacgcc agttcacaag cactaaaaaa ctgcgatccg cgacgcatgt tgcaaaaata
    37261 atgcgtaatt atgccgttac gcttgccaaa cgttcctgca caatggcgat gaaatcgcgt
    37321 aatgccggct tcatctcccc tttcttccac gccatcagta aagcaatgga aggcacattt
    37381 ccggcaatgg gacgaaaaac aacctgtccg gtattaaaat tattcatata accgggtatc
    37441 aaagtgacgc ccagccccat gcccaccaga ttcatggtca ccagaatatt cgttgccacc
    37501 tggacgatat ttggctggct gttttcttgc gcaaaccacg ctttaacgat cggcgcaagc
    37561 gaaccggaat acgccggatc ggtactgacg aaattcacgc catccagttg cgcggcggtg
    37621 atctcttttt catgtgctaa agggtgatca accggtaaca caaccactaa tggttcgtca
    37681 aaaagctcca gataatcaat ctccgggcta taaacaggat ggcgcattaa gccgacgtcg
    37741 agttcaccac ggcgaatttt ttcctcctgt tgcgtggtga ttaaactcac cagctcaatc
    37801 aaggtgtctg gctgtctgag acgaaacatc ggtaatactt ttggcagtaa attcacttcc
    37861 gccgatggca caaagccaat ggttaattgt ctgtcttcct gaacagtttt ccgcgcccgt
    37921 aatttggcat tttccgcttg ttcgaggatt gccagcgcgt cctggagaaa acattctccc
    37981 gccgccgtca acgcgacttt gcgcttatcc ctcaccagta acggaacacc gacacagttt
    38041 tcaagatcgc ggatctggct gcttaacgaa ggctgtgagg tatgcagttt ttccgccgca
    38101 cgggtaaagt tcagtgcctg cgccactgcg acgaaatagc gtaaatgccg tagttccatc
    38161 accttcccct tgttatcgaa aaaacgtctc agctggtaga aattaactat ttcacatatt
    38221 agcaaccaac cagcaacatc cttatgacac aaaaatagaa ggtcaataca tcttatcttt
    38281 caggattaaa aaatatgacc acaccctcag atttgaacat ttaccaactg attgacaccc
    38341 aaaatggtcg ggtcactccg cgtatttata ccgacccgga tatttaccaa ctggagcttg
    38401 agcgtatttt cggtcgttgc tggctatttc tcgcccacga aagccagatc ccaaaacccg
    38461 gtgatttctt taacacctac atgggagaag atgcggttgt cgtagtgcgt cagaaagacg
    38521 gcagcatcaa ggcgtttctc aaccaatgcc gccaccgggc catgcgtgtg agttatgcag
    38581 attgcggcaa cactcgcgcc tttacctgcc cgtatcacgg ctggtcttat ggcattaacg
    38641 gcgagttgat cgatgtaccg ctggaacctc gcgcctaccc acaagggttg tgtaaatccc
    38701 actgggggct aaacgaagtt ccttgtgtgg agagttataa agggctgatt tttggcaact
    38761 gggataccag cgcaccgggc ctgcgtgatt accttggtga cattgcctgg tatctggatg
    38821 gcatgctgga tcgtcgcgaa ggcggcaccg aaattgtcgg cggcgtacaa aagtgggtga
    38881 tcaactgtaa ctggaaatcc ccggcagagc agttcgccag tgaccaatat catgctctgt
    38941 tcagccatgc ttctgccgtt caggtattag gggcgaaaga tgatggcagc gataagcgtc
    39001 tcggtgatgg acaaaccgcc cgcccggtgt gggaaaccgc caaagatgcg ctgcaatttg
    39061 gtcaggacgg tcacggtagc ggtttcttct ttactgaaaa accggatgct aatgtctggg
    39121 tcgatggcgc ggtttccagc tattaccgcg aaacctatgc cgaagcagaa caacgtttag
    39181 gtgaagttcg cgccctgcgc ctggcgggtc ataacaatat attccccacg ctttcatggc
    39241 taaacggcac tgccacactc cgcgtctggc atccgcgcgg ccctgatcaa gttgaagtgt
    39301 gggcgttctg tattactgac aaagccgcct ccgatgaagt taaagccgct tttgaaaaca
    39361 gcgccactcg tgcttttggt cctgctggtt ttctcgagca ggatgactcg gagaactggt
    39421 gtgaaatcca gaaattgctt aaaggccacc gcgcccgcaa cagcaaactg tgtctggaaa
    39481 tggggcttgg tcaggaaaag cgccgcgacg acggcattcc tggcattact aactatatct
    39541 tttcagaaac tgccgctcgt ggaatgtacc aacgctgggc cgatcttctg agtagcgaaa
    39601 gctggcagga agtgctcgat aaaaccgccg cttaccagca ggaggtgatg aaatgagtgc
    39661 gcaagtttca ctagagttac atctccgcat tagccagttt ctctttcacg aagccagctt
    39721 actggacgac tggaaatttc gtgactggct ggcgcagctc gacgaagaga ttcgttacac
    39781 catgcgcacc acagttaacg cgcaaacacg cgaccgccgc aaaggcgtcc agccaccgac
    39841 aacctggatt tttaatgaca ccaaagacca gctggagcgg cgaatcgccc gtctggaaac
    39901 gggcatggcc tgggcagaag agccgccgtc acgcacccgt cacttaatca gcaactgcca
    39961 gataagcgaa accgacatcc caaacgtatt tgctgtgcgg gtaaattatc tgctttatca
    40021 ggcacaaaaa gagcgcgatg aaacattcta tgttggaacg cgtttcgaca aagttcgccg
    40081 tctggaagat gacaactggc gcttgctgga acgggatatc gtcctggatc aagcggtaat
    40141 cacttcccat aacctgagtg tactgttctg atgaatcgaa tttatgcgtg tcccgttgcg
    40201 gatgtgccgg agggtgaggc tctccggatc gatacctcgc ccgtcatcgc cctgttcaac
    40261 gttggcggcg agttttatgc cattaacgat cgttgcagcc atggtaatgc gtcgatgtca
    40321 gaagggtatc tggaagatga cgccacggtg gagtgcccgc tacacgccgc cagtttttgc
    40381 ctgaaaacgg ggaaagcgtt atgcctgccc gccaccgatc cgctcaccac ttatccagta
    40441 cacgttgaag gtagtgacat tttcatcgac ttaccggagg cgcagccatg agcgatctgc
    40501 ataacgagtc catttttatt accggcggcg gatcgggatt agggctggcg ctggtcgagc
    40561 gatttatcga agaaggcgcg caggttgcca cgctggaact gtcggcggca aaagtcgcca
    40621 gtctgcgtca gcgatttggc gaacatattc tggcggtgga aggtaacgtg acctgttatg
    40681 ccgattatca acgcgcggtc gatcagatcc tgactcgttc cggcaagctg gattgtttta
    40741 tcggcaatgc aggcatctgg gatcacaatg cctcactggt taatactccc gcagagacgc
    40801 tcgaaaccgg cttccacgag ctgtttaacg tcaacgtact cggttacctg ctgggcgcaa
    40861 aagcctgcgc tccggcgtta atcgccagtg aaggcagcat gattttcaca ctgtcaaatg
    40921 ccgcctggta tcctggcggc ggtggcccgc tgtacaccgc cagtaaacat gccgcaaccg
    40981 gacttattcg ccaactggct tatgaactgg caccgaaagt gcgggtgaat ggcgtcggcc
    41041 cgtgtggtat ggccagcgac ctgcgcggcc cacaggcgct cgggcaaagt gaaacctcga
    41101 taatgcagtc tctgacgccg gagaaaattg ccgccatttt accgctgcaa tttttcccgc
    41161 aaccggcgga ttttacgggg ccgtatgtga tgttggcatc gcggcgcaat aatcgcgcat
    41221 taagcggtgt gatgatcaac gctgatgcgg gtttagcgat tcgcggcatt cgccacgtag
    41281 cggctgggct ggatctttaa ggaagcacga tgaaagaaaa aacgatcatt attgtcggtg
    41341 gcgtgcaagc ggcggcaatg gctgcggcct cgctacgcca gcaagggttc accggtgagc
    41401 tgcatctgtt ttccgatgag cgacatcttc cttatgaacg acctccactc tcgaaatcca
    41461 tgttgctgga agattccccg cagttacagc aggtgttacc cgctaactgg tggcaggaaa
    41521 acaatgttca tctgcattcc ggtgtaacca tcaaaacgct gggtcgcgac acacgagagt
    41581 tagtgttaac caacggcgaa agctggcact gggatcagct ttttatagca accggcgcgg
    41641 cagctcgacc gctgccgttg cttgatgcac tgggagaacg ctgctttacc ctacgccatg
    41701 ccggtgatgc cgccagactg cgagaagttc tgcagcccga acggtcagtc gtgattgtcg
    41761 gtgccggaac tattggtctg gaactggctg ccagcgccac gcagcgcagc gcagcgcaga
    41821 tgtaaggtga cagtgattga actggcggca accgtcatgg gccgtaatgc accaccgccc
    41881 gtgcaacgct atcttttaca gcgccaccag caggctggtg tgcgcattct gctcaataat
    41941 gccattgaac atgtagtcga tggtgaaaaa gtagaactga cgctgcaaag tggggaaacg
    42001 cttcaggctg atgtggtgat ttacggtatt ggtatcagcg ccaacgagca actggctcgc
    42061 gaggccaacc ttgatactgc caatggcatt gtcattgatg aggcttgccg cacctgcgat
    42121 cccgcgatct ttgccggtgg cgatgtggca atcactcgtc ttgataatgg tgcactacac
    42181 cgctgtgaaa gctgggaaaa cgccaataac caggcgcaaa ttgccgctgc cgcaatgtta
    42241 gggctaccgc taccgctact gccgtggttc tggagcgatc agtacagtga taacttacag
    42301 tttattggcg atatgcgtgg cgatgactga ggtagcctga gtttaacgga cactccttcc
    42361 tgaaatagaa tggcatca
//
LOCUS       DAZPEB010000011        40426 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZPEB010000011 DAZPEB010000000
VERSION     DAZPEB010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40426
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.011"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(116..2437)
                     /locus_tag="ACHURJ_000561"
     CDS             complement(116..2437)
                     /locus_tag="ACHURJ_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFR1279633.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFITRYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(2534..3010)
                     /gene="greB"
                     /locus_tag="ACHURJ_000562"
     CDS             complement(2534..3010)
                     /gene="greB"
                     /locus_tag="ACHURJ_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFR1279634.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            3238..3957
                     /gene="ompR"
                     /locus_tag="ACHURJ_000563"
     CDS             3238..3957
                     /gene="ompR"
                     /locus_tag="ACHURJ_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFR1279635.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            3954..5306
                     /gene="envZ"
                     /locus_tag="ACHURJ_000564"
     CDS             3954..5306
                     /gene="envZ"
                     /locus_tag="ACHURJ_000564"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFR1279636.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(5382..7004)
                     /gene="pckA"
                     /locus_tag="ACHURJ_000565"
     CDS             complement(5382..7004)
                     /gene="pckA"
                     /locus_tag="ACHURJ_000565"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFR1279637.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            7383..9107
                     /locus_tag="ACHURJ_000566"
     CDS             7383..9107
                     /locus_tag="ACHURJ_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HFR1279638.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNCSSRYSYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWGELFKLVGIKFFNTLFFATDWFMYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDVTFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            complement(9242..10120)
                     /gene="hslO"
                     /locus_tag="ACHURJ_000567"
     CDS             complement(9242..10120)
                     /gene="hslO"
                     /locus_tag="ACHURJ_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFR1279639.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSREHCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(10145..10546)
                     /gene="hslR"
                     /locus_tag="ACHURJ_000568"
     CDS             complement(10145..10546)
                     /gene="hslR"
                     /locus_tag="ACHURJ_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFR1279640.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(10557..11225)
                     /gene="yrfG"
                     /locus_tag="ACHURJ_000569"
     CDS             complement(10557..11225)
                     /gene="yrfG"
                     /locus_tag="ACHURJ_000569"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFR1279641.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(11290..13425)
                     /gene="igaA"
                     /locus_tag="ACHURJ_000570"
     CDS             complement(11290..13425)
                     /gene="igaA"
                     /locus_tag="ACHURJ_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFR1279642.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRSQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLDQQTDIDIYLDCHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            13745..14305
                     /gene="nudE"
                     /locus_tag="ACHURJ_000571"
     CDS             13745..14305
                     /gene="nudE"
                     /locus_tag="ACHURJ_000571"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFR1279643.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(14470..17022)
                     /gene="mrcA"
                     /locus_tag="ACHURJ_000572"
     CDS             complement(14470..17022)
                     /gene="mrcA"
                     /locus_tag="ACHURJ_000572"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFR1279644.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            17142..17921
                     /gene="hofM"
                     /locus_tag="ACHURJ_000573"
     CDS             17142..17921
                     /gene="hofM"
                     /locus_tag="ACHURJ_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFR1279645.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALFVDPDS
                     VAICGEGGFDPWEAVSVRQPLLPPPGGDFAIALGLALGKAY"
     gene            17921..18460
                     /gene="hofN"
                     /locus_tag="ACHURJ_000574"
     CDS             17921..18460
                     /gene="hofN"
                     /locus_tag="ACHURJ_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFR1279646.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLRKQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            18444..18884
                     /gene="hofO"
                     /locus_tag="ACHURJ_000575"
     CDS             18444..18884
                     /gene="hofO"
                     /locus_tag="ACHURJ_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFR1279647.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            18874..19278
                     /gene="hofP"
                     /locus_tag="ACHURJ_000576"
     CDS             18874..19278
                     /gene="hofP"
                     /locus_tag="ACHURJ_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFR1279648.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            19190..20428
                     /gene="hofQ"
                     /locus_tag="ACHURJ_000577"
     CDS             19190..20428
                     /gene="hofQ"
                     /locus_tag="ACHURJ_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFR1279649.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGIEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEYERRELVVFITPRLVSSE"
     gene            20829..21350
                     /gene="aroK"
                     /locus_tag="ACHURJ_000578"
     CDS             20829..21350
                     /gene="aroK"
                     /locus_tag="ACHURJ_000578"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFR1279650.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            21407..22495
                     /gene="aroB"
                     /locus_tag="ACHURJ_000579"
     CDS             21407..22495
                     /gene="aroB"
                     /locus_tag="ACHURJ_000579"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFR1279651.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            22587..23873
                     /gene="damX"
                     /locus_tag="ACHURJ_000580"
     CDS             22587..23873
                     /gene="damX"
                     /locus_tag="ACHURJ_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFR1279652.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGDKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            23980..24816
                     /gene="dam"
                     /locus_tag="ACHURJ_000581"
     CDS             23980..24816
                     /gene="dam"
                     /locus_tag="ACHURJ_000581"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFR1279653.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            24834..25511
                     /gene="rpe"
                     /locus_tag="ACHURJ_000582"
     CDS             24834..25511
                     /gene="rpe"
                     /locus_tag="ACHURJ_000582"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFR1279654.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            25504..26262
                     /gene="gph"
                     /locus_tag="ACHURJ_000583"
     CDS             25504..26262
                     /gene="gph"
                     /locus_tag="ACHURJ_000583"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFR1279655.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALAWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     SEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            26255..27259
                     /gene="trpS"
                     /locus_tag="ACHURJ_000584"
     CDS             26255..27259
                     /gene="trpS"
                     /locus_tag="ACHURJ_000584"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFR1279656.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            27528..28433
                     /gene="yhfZ"
                     /locus_tag="ACHURJ_000585"
     CDS             27528..28433
                     /gene="yhfZ"
                     /locus_tag="ACHURJ_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFR1279657.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            28450..28812
                     /locus_tag="ACHURJ_000586"
     CDS             28450..28812
                     /locus_tag="ACHURJ_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFR1279658.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            28896..30059
                     /locus_tag="ACHURJ_000587"
     CDS             28896..30059
                     /locus_tag="ACHURJ_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HFR1279659.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLSVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRVQFVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLIQAR
                     DQLAKSGIALEQLNAPSATSCTSLPLLAQYGVTHAEPGHALTGTIPANQQGDQSERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     SLAGEFPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPKIVGRYDSLGNSLGA"
     gene            30059..31285
                     /locus_tag="ACHURJ_000588"
     CDS             30059..31285
                     /locus_tag="ACHURJ_000588"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFR1279660.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFCDVIDRVEQALVSAGWQVERRGDELQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDKKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLARLVDAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPLLVYQQGLAATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            31282..32160
                     /locus_tag="ACHURJ_000589"
     CDS             31282..32160
                     /locus_tag="ACHURJ_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFR1279661.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLGVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGLIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            32171..32524
                     /locus_tag="ACHURJ_000590"
     CDS             32171..32524
                     /locus_tag="ACHURJ_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFR1279662.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            32536..33840
                     /locus_tag="ACHURJ_000591"
     CDS             32536..33840
                     /locus_tag="ACHURJ_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFR1279663.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            33852..34937
                     /locus_tag="ACHURJ_000592"
     CDS             33852..34937
                     /locus_tag="ACHURJ_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFR1279664.1"
                     /translation="MKTFPLQSLTLIEAQQKQFALVDTICRHFPGAEFLTSGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITVDFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVINRIRA
                     TLYSGGSQIQGAQALEVLRCLVFAPVMHAVQAGVSERLLALLNGGEVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(35089..35820)
                     /gene="frlR"
                     /locus_tag="ACHURJ_000593"
                     /gene_synonym="yhfR"
     CDS             complement(35089..35820)
                     /gene="frlR"
                     /locus_tag="ACHURJ_000593"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFR1279665.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCKKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(35920..36705)
                     /gene="frlD"
                     /locus_tag="ACHURJ_000594"
     CDS             complement(35920..36705)
                     /gene="frlD"
                     /locus_tag="ACHURJ_000594"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFR1279666.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDETLRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            complement(36702..37532)
                     /gene="frlC"
                     /locus_tag="ACHURJ_000595"
     CDS             complement(36702..37532)
                     /gene="frlC"
                     /locus_tag="ACHURJ_000595"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HFR1279667.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELYEYAENIGMDLILEPLTPYESNIVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIERGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            complement(37582..38604)
                     /gene="frlB"
                     /locus_tag="ACHURJ_000596"
     CDS             complement(37582..38604)
                     /gene="frlB"
                     /locus_tag="ACHURJ_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFR1279668.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQAAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            complement(38625..39962)
                     /gene="frlA"
                     /locus_tag="ACHURJ_000597"
     CDS             complement(38625..39962)
                     /gene="frlA"
                     /locus_tag="ACHURJ_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HFR1279669.1"
                     /translation="MGSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWL
                     TVLTFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDA
                     PSLSIMALAIVSNLGFLTPIDPLPGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAK
                     IIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGISATSWSYTGMASICYMT
                     GEIKNPGKTMPRALIGSCLLVLVLYTLLALVISDLMPFDKLANSETPISDALTWIPAL
                     GSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSI
                     ILQGALGLFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGL
                     MTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
     gene            complement(40258..40425)
                     /locus_tag="ACHURJ_000598"
     CDS             complement(40258..40425)
                     /locus_tag="ACHURJ_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFR1279670.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
BASE COUNT         9347 a        10256 c        11119 g         9704 t
ORIGIN      
        1 ttgtggaata aaaaggccgg agatattctc cggcactttt atgtcaggca aggcgttcgt
       61 tgccagatgc ggcgtgaacg ccttatcgcg caggtttagg ggccagtccg ggcgtttaac
      121 gttttttgcc cattgccgcc gccagcgcat ccatcatcgc gctattaccg gcaggctgcg
      181 cttcacgacc gcgtggtttg gctgccgggc ggttgttttg cgggcgttca ttaccgccgc
      241 cgcgacgggc gttggtttcg ccaggctgct cgtccagacg catagtcagg gcgatacgtt
      301 tacgctgaag atccacttcc agcactttca ccttcacaat gtcgcccgct ttcaccacgg
      361 tatgcggatc ttccacaaac ttgttcgaca atgaagagat gtgaaccagg ccgtcctgat
      421 gcacgccgat atcgacaaac gcgccaaagt tggtgacgtt ggtcaccgca ccttcgagga
      481 tcatacccgg ttgcaggtcg ttcattgtct cgacgccatc ggcaaactga gcggttttaa
      541 attccggacg cggatcgcga cccggttttt ccagctcttt gatgatgtca gttactgtcg
      601 gcacaccgaa tttttcatca gtaaagtcag acgctttcag gttacgcagt tcgctgctgt
      661 tacccatcag atctttcagt gcctgctgtg ttgctgccag aatgcgttcc accaccggat
      721 aggcttccgg gtggacggta gaagcatcca gcgggttatc accgtggtta atgcgcaaga
      781 agcccgcgca ctgctcgaag gctttcggcc ccagacggct gactttcagc agttgctgac
      841 ggttctggaa ctggccgttc tcatcgcgcc aggccacgat gttttgcgcc atcatgcgcg
      901 tcaggcccgc cacgcgggtt aacagcggaa cagaagcagt gttgagatcg acgccaacgg
      961 cgtttacgca gtcttctact actgcgtcca gtttgcgggc cagttgcgtc tggctgacgt
     1021 catgctgata ctgacctacg ccgatagatt tcggatcgat tttcaccagc tccgccagcg
     1081 gatcctgcaa acggcgggcg atagacaccg cgccacgcag cgaaacgtcg agatccggga
     1141 actcctgcgc tgccagctcg gaagctgagt aaaccgacgc gccagcttcg ctgacgatca
     1201 ctttctgtgc ggtcactttc gggaactgct tctgcacgtc gagatagaaa cgttcagttt
     1261 cgcgggaagc tgtaccgttg ccgatcgcca ccagttcaac gttatgtttt tcacacaagg
     1321 cagcaacggt catcgctgct tttgcggctt gtccggtgtg cgggtaaatg gtatcggtcg
     1381 ctaccagttt gccagtggca tcgaccaccg ccacttttac cccagtacgc agacccggat
     1441 cgaggcccat cgttgcacgc agtccggcag gggccgccat cagcagatcg tgcaggttac
     1501 gggcaaagac gttgattgct tcatcttccg cgcgttcgcg cacggtaccc atcagttcgg
     1561 tttccagatg catcagcacc ttgatgcgcc aggtccagct cactacgcct ttgcgccagc
     1621 tatccgccgg ggcattgttc aggcgcaggc caaggtgatc catgatgatt tgctcgcaat
     1681 agctctcttt gggcggctca tcgaactgcg gatcggcatt cagcgaaagc tggagtacgc
     1741 cttcgttacg cccacggaac atcgccagag cgcggtgaga aggcaccgtg gacaacggtt
     1801 cgtgatgatc gaaatagtcg cggaatttcg ccccttcctc ttctttaccg ctcaccaccg
     1861 tagaaaccaa atgcgcgttc ttccacagat aatcacgcac tttcgccagc agcgcggcat
     1921 cttcggcaaa ccgttccatc aggatatagc gcgcgccatc cagcgcggct ttggtatctg
     1981 ccacgccttt atcggcatca acatattgtg cagcggcgac ttctggcgtg tgtgacggat
     2041 cgctccacag caggtcagcc aacggctcaa gccctgcttc aatggcgatt tgcccgcggg
     2101 tgcggcgttt aggtttgtag ggcaggtaga ggtcttcgag ttcggttttg cttagggtgg
     2161 cgttgatggc cttcgccaga tcatcggtga gtttgccttg ctcggaaatg gacttgagga
     2221 tcgcctgacg tctctcttcc agctcgcgca gatagctcag acgcgtttcc agattacgca
     2281 gctgcgtgtc atccagaccg ccggtgattt ccttacgata acgtgtgata aacggcacgg
     2341 tattcccttc gtcaagcagg cgaacggcag cgtcaacctg ttccgggcgc gcctgaattt
     2401 cacccgcaat aatgcggcag aacgaatcat tcatcatggt ttgagttcat cttttcggat
     2461 caaaaatcag gggatagtta tacggactgg ctggcaaaaa tgccagccat cggcaggagg
     2521 ttaagactct tccttacggt ttcacgtact cgatagcatt aacataccag ctcgcttccc
     2581 cggcaggggt attcaccacc gccagatcgc cgacttcttt tttcagcaat gcgcgggcca
     2641 tcggggaatc gatagagatg taatctttac ggccaaaaat ttcatcgtag ccgacaatac
     2701 ggaaacggtg agtcacgcca tcgtcgtttt caatctccac ccacgcgcca aaaaagactt
     2761 tgccttcctg ctgaggggag taatcgacga ttttgagatt ttccatgcat ttagtgagat
     2821 agcgcacgcg acggtcgatt tcacgcagac gctttttatt atactgatag tcagcatttt
     2881 cgctgcggtc gcccagactt gcggcccagg tcaccttttt tgtgacctcc gggcgttctt
     2941 cacgccagag ataattaagc tcttgtttga gtttttcata cccttcccgg gtaaccaggg
     3001 gcgttttcat ctcgttgatt ccctttgtct gtttgataat gcgcacattg ggtataacgt
     3061 gatcatatca acagaatcaa taatgtttcg ccgaataaat tgtatactta agctgctgtt
     3121 taatatgctt tgtaacaatt taggctgaaa ttcataccag atttagctgg tgacgaacgt
     3181 gagctttttt aagaatacac gcttacaaat tgttgcgaac ctttgggagt acaaacaatg
     3241 caagagaact acaagattct ggtggtcgat gacgacatgc gcctgcgtgc gctgctggaa
     3301 cgttatctca ccgaacaagg cttccaggtt cgaagcgtcg ctaatgcaga acagatggat
     3361 cgcctgctga ctcgtgaatc tttccatctt atggtactgg atttaatgtt acctggtgaa
     3421 gatggcttgt cgatttgccg acgtcttcgt agtcagagca acccgatgcc gatcattatg
     3481 gtgacggcga aaggggaaga agtggaccgt atcgtaggcc tggagattgg cgctgacgac
     3541 tacattccaa aaccgtttaa cccgcgtgaa ctgctggccc gtattcgtgc ggtgctgcgt
     3601 cgtcaggcga acgaactgcc aggcgcaccg tcacaggaag aggcggtaat tgctttcggt
     3661 aagttcaaac ttaacctcgg tacgcgcgaa atgttccgcg aagatgagcc gatgccgctc
     3721 accagcggtg agtttgcggt actgaaggca ctggtcagcc atccgcgtga gccgctctcc
     3781 cgcgataagc tgatgaacct tgcccgtggt cgtgaatatt ccgcaatgga acgctccatc
     3841 gacgtgcaga tttcgcgtct gcgccgcatg gtagaagaag atccagcgca tccgcgttac
     3901 attcagaccg tttggggcct gggctacgtc tttgtaccgg acggctctaa agcatgaggc
     3961 gattgcgctt ctcgccacga agttcatttg cccgtacgtt attgctcatc gtcaccttgc
     4021 tgttcgccag cctggtgacg acttatctgg tggtgctgaa cttcgcgatt ttgccgagcc
     4081 tccagcagtt taataaagtc ctcgcgtacg aagtgcgtat gttgatgacc gacaaactgc
     4141 aactggagga cggcacgcag ttggttgtgc ctcccgcttt ccgtcgggag atctaccgtg
     4201 agctggggat ctctctctac tccaacgagg ctgccgaaga ggcaggtctg cgttgggcgc
     4261 aacactatga attcttaagc catcagatgg cacagcaact gggcggcccg acggaagtgc
     4321 gcgttgaggt caacaaaagt tcgcctgtcg tctggctgaa aacctggctg tcgcccaata
     4381 tctgggtacg cgtgccgctg accgaaattc atcagggcga tttctctccg ctgttccgct
     4441 atacgctggc gattatgcta ttggcgatag gcggggcgtg gctgtttatt cgtatccaga
     4501 accgaccgtt ggtcgatctc gaacatgctg ctttgcaggt cggtaaaggg attattccac
     4561 cgccgctgcg tgaatatggc gcgtccgagg tacgttcggt tacccgcgcc tttaaccata
     4621 tggcggctgg tgttaagcaa ctggcggatg accgcacgct gctgatggcg ggggtaagtc
     4681 atgacttacg cacgccgctg acgcgtattc gcctggcgac cgagatgatg agcgaacagg
     4741 atggctacct ggccgaatcg atcaataaag atatcgaaga gtgcaacgcc atcattgagc
     4801 agtttatcga ctacctgcgc accgggcagg agatgccgat ggaaatggcg gatctcaacg
     4861 cagtactcgg tgaggttatt gctgccgaaa gtggctatga gcgggaaatt gaaaccgcgc
     4921 tttaccccgg cagcattgaa gtgaaaatgc acccgctgtc gatcaaacgc gcggtggcga
     4981 atatggtggt caacgccgcc cgttacggca atggctggat caaagtcagc agcgggacgg
     5041 agccgaatcg cgcctggttc caggtggaag atgacggtcc gggaattgcg ccggaacaac
     5101 gtaagcacct gttccagccg tttgtccgcg gcgacagtgc acgcaccatt agcggcacgg
     5161 gattagggct ggcaattgtg cagcgtatcg tggataacca taacgggatg ctggagcttg
     5221 gcaccagcga gcggggcggg ctttccattc gcgcctggct gccagtgccg gtaacgcggg
     5281 cgcagggcac gacaaaagaa gggtaaataa acgggaggcg aaggtgcctc ccgttttgct
     5341 ttctataaga tactggatag atattctcca gcttcaaatc attacagttt cggaccagcc
     5401 gctaccagcg cggcacccgc aggggtgtcg gtgtatttat cgaagttgtc gataaacagt
     5461 ttcgccaggg tttcggcttt ttcctgccac tgttccggag aagcgtaggt gttacgcgga
     5521 tcgagaatct tcgtgtctac gcccggcagt tcggttggga tcgccaggtt aaacatcggc
     5581 agagtgaagg tttctgcatt atccagcgaa ccgttgagga tggcgtcgat aatggcgcgg
     5641 gtatctttaa tcgagatacg tttgcctgtg ccgttccagc cagtgttaac cagataagcc
     5701 tgcgcgcccg ccgcctgcat acgtttcacc agcacttctg cgtactgagt cgggtgcagc
     5761 gacaggaatg ccgcgccgaa gcaagcggag aaggttggcg tcggttcggt gatgccacgc
     5821 tcagtaccgg ccagtttggc ggtgaagcca gagaggaagt gatactgggt ttgatcggca
     5881 gtcaggcgag aaaccggcgg caacacgccg aaagcatcag cagtcaggaa gataacctta
     5941 gtcgcgtggc ccgctttgga aaccggctta acgatgttat cgatgtgata gattggataa
     6001 gaaacgcggg tgttctcggt ttttgaacca tcatcaaagt cgatagtgcc atcttcacgc
     6061 acggtgacgt tttccagcag cgcatcacga cggatagcgt tgtagatttc aggttccgct
     6121 tctttcgaca gcttgatagt ttttgcgtag cagccgcctt cgaaattaaa cacgccgtca
     6181 tcgtcccagc cgtgttcgtc atcgccaatc aggcggcgtt tcgggtcggt ggaaagggtg
     6241 gttttaccgg tgccggaaag gccgaagaac accgcaacat cgcctttctc gccaacgttg
     6301 gcggagcagt gcatagaagc gatacctttc agcggcagca ggtagttcat catcgagaac
     6361 atccctttct tcatttcgcc gccgtaccag gtgccgccaa tcagctgcat gcgctcggtc
     6421 aggttaaacg ccacgaagtt ttcggagttc agaccctgtt ctttccactg cgggttagtg
     6481 cacttcgcgc cgttcataac gataaagtct ggtttgaaac ctgccagctc ttcatcgctc
     6541 gggcgaataa acatgttttt gacaaaatgc gcctgccagg ccacttcggt gatgaaacgg
     6601 acggaaagac gagtatccgg gttcgcacca cagaaagcgt cgacaacgaa cagacgtttg
     6661 ccggaaagct gcctggtcac caggcctttc agatgctgcc aggtttccgg agagagaggt
     6721 ttgttgtcgt tcttaccttt gcctttgtct gcccaccaga aagtatcgcg agtggtatcg
     6781 tcacggacga tatacttatc ttttggtgaa cgaccggtga agatcccggt atcgacggca
     6841 acggcaccca gattagttaa caccccgcgc tcataacctg tcaggctcgg atcgagctct
     6901 tcctgataca gcaggtcgta gcttgggttg taaacgatat catgtacgtc actgatacca
     6961 taagcctcga gttcttgcgg ggtcaaacca ttgttaacgc gcatttcact gctccttagc
     7021 caatatgtat tgcctgaata gtaaagtctt tttgggggtg ttaaccgcga caaggctcat
     7081 agatttacgt atctggagaa attcatattt gacaaaaaac cccgtgattc ctgttacgaa
     7141 acggttgcta ttatcgcaga aatagcattt taggtgtgga aaatgttctc aaaaggttaa
     7201 atcttggttt atatggcggg aatattgtga gtggaatcgc attccttaat gaaattgatg
     7261 caaattcaat cggtaaagta tcgattcacc acgctaactt tttttgatta ttttctgaaa
     7321 aaagcaaaat actactgccc ggatgcgata attaacccac cttttgattc tggataacca
     7381 acatggataa cgttgaactt tcaccggcaa cacgctgggg gatgattgct accggattac
     7441 ttcaggggct ggtttgctac ctgttgatcg cctggcttgc cggaaaaaat cacagttgga
     7501 ttgtttatgg cgtgcctgcg accgttgcgt tttcatccgt tttactcttc tccgtgatct
     7561 cttttaaaca gaagcgcctt tggggatggc tggcgctggt gtttattgcc acgacaggga
     7621 tgagcggttg gctgaagtgg caaactgatg gcatgaaccc ctggagagct gaaaaggcga
     7681 tttgggattt tggctgctat ctgctgttga tggcaatgtt gttgctaccg tggatacaac
     7741 aaagccttcg tatccgcaat tgcagtagcc gctatagcta cttttaccag tcagtatggc
     7801 ataacgtact tatattactg gtgatttttc tcgccaatgg cctgacgtgg ctggtgcttt
     7861 tactgtgggg tgagttgttt aaacttgttg gcatcaagtt tttcaatacg ctcttttttg
     7921 caaccgactg gtttatgtat cttacattag gtctggttac cgcactggcg gtgattctcg
     7981 cgcgcacaca gtcacgttta atcgactcta ttcaaaagtt gttcacgtta atcgccacgg
     8041 ggttgctgcc gttagtatca ttgctaaccc tgatgtttat catcactctg ccgtttacgg
     8101 gcctgagcgc gatttctcgc cacatctccg ccgccgggtt gctgttaacg ctggcctttt
     8161 tgcaattgat cttaatggct attgtccgcg atccgcaaaa agcgtcactt ccctggacgg
     8221 ggccgttgcg ttgcctgatt aaaaccgctt tgctggttgc tccgctgtat gtgttcgtcg
     8281 ccgcctgggc gttatggctg cgggtcgctc agtacggctg gactgtcgac cgcttgcagg
     8341 gcgcgctggc ggtgctggtg ttactggtgt ggtcgctggg gtattttgtc agtatcgtct
     8401 ggcgtaatgg gcaaaatccc cttgttcttc agggcaaagt gaaccttgcg gtttcgttat
     8461 tggtgttggt gatactggtg cttcttaatt cgccggtgct ggacagtatg cgtattagcg
     8521 tgaacagtca tatggcgcgt tatcagagcg gcaaaaacac gccagaccag gtaactatct
     8581 acatgctcga acagagcggt cgctatggac gtgcggcgct tgagtcgctg aaaagcgatg
     8641 ccgggtttat gaaagacccg aaacgcgcgc gggatctgct gatggcgtta gatggagagc
     8701 aacatcttca ggaacaggta tcggaaaaag tattagccga aaatgtgtta attgcccctg
     8761 gttctgttaa accggatgtg acattctggt cggccttaat ccaggatcgc tataacgtga
     8821 tgacctgtat tgaaaaagac gcctgcgtcc tggtcgagca agatctgaat agtgatggtc
     8881 aggcggagcg gatcctgttt gcttttaatg atgacagagt cattgtctat ggctttgact
     8941 cagacagaaa agaatgggac gcgcttgata tgagtttact tccgaacgaa ataacgaaag
     9001 aaaaattact cacagctgcg aaggatggaa aactggggac gaggccgaaa gcgtggcgcg
     9061 atctgacggt agatggtgaa acgctggaaa taaacttaag taagtgacaa cgcttttatc
     9121 ggatacgacg ctgtatctga ccaaaataat ttgcgattta tttcataggc gctggtaagc
     9181 gcagcacatc aggccattaa gttgtaaaat ccggcagagc cctctctgcc ggacatactc
     9241 attaatgaac ttgcggatct gccggagacg cgttgttgcg gatttcagca atatccatcg
     9301 cattgaacag atagtggtta ccgcagtaat cacaatgcat gtcaatttcg ccatcttccg
     9361 ccaggatgct atcaacttct tcatcaggca gcgttttcag cgcatcggcg caatgttcac
     9421 gcgagcaggt gcatttgaac tccacatcct gcggatcgta aaccgtcacc tcttcttcgt
     9481 gatacaaacg ccacaacact tcgtttgccg gtaaggtcag cagttcttcg gttttgatgg
     9541 tttcggttag cgtcgccagg tggtcaaagt cgtcctgctg ggcattttgc gcaggcatta
     9601 cctgcaacaa cataccgcct gcagccggtt tgccgtctac gtcgccggtg cgaataaaca
     9661 ggcgcgtcgg cagctgttca gaacgcataa agtaatcttc caggcaggcc gccagggtat
     9721 caccttccag accaactacg ccctgatagc gttcgccttc gctcggggta atggtgatca
     9781 ccacgtaacc attgccgacc agcgttttca ggtcggcatt ttctggaatt tcgccctgca
     9841 cgcgcgccac accgcgcatt tgctggttat tgttaccgtt aataaccgcc agattcatcg
     9901 gaccgtcgcc ctgcagctgt acggtgatat caccatcaaa cttcagcgta gcggttaaca
     9961 ggctggtcgc aaccagcagt tctgccagca cgtttttaac gggctgcgga taatcgtggt
    10021 tctcaaggat ctgttgcagg gtttccgaaa cggttaccag ttcgccgcga acggcaaaat
    10081 tttcaaacag atagcgatgt aattggtcat gttgcggcat aatcatctct cttgcaggtg
    10141 acagttattc actgtcgccg tgtttaaatc gtaacaggtc gcggcgctct tttttgtccg
    10201 ggcgtcggtc cgggtgcggc atggttaagg cattaagttt acgtgccatc gccatttttt
    10261 cgcgtttctc tacactttcc gcagtctctt catacagcaa ggctgcctcg ctggcggggc
    10321 gacgctgttc agtaatcgcc tttacaatca ccgtgcgttc gtcatttccc tggcgcagag
    10381 tgagcgtggc attcagctcg acgattttgc tcggcttgct gcgctgcccg ttgtaatgca
    10441 ccttaccgcc ttcaatcatt tcacgggcca gcgcgcgggt tttataaaaa cgggcagccc
    10501 atagccattt atccagtcga acctcaacag caggtttctc tttcatggcg tctccttcac
    10561 attagcgagg ggatcaggcg gcggtagtca ttcagtgacg gatggcgttg atactgtttc
    10621 tcggcaatcc cggaatcagg attagtcacg ccgaggcagt aacgaatacc aaattgcgcg
    10681 gcagcatcga gaatcgcttc gctgtcatca ataaacagcg ttctttcagc tttcagaccc
    10741 gtagcttcgg ccaccgcatg ccataaccgc tgatcctctt tcggataacc aaatgtgtgg
    10801 gtggaaagta ataaatcaag gtgtgcgtcc agacccgtat gctcaagttt taccgccagg
    10861 ttgtgcggat gagcattggt gagcaaaatt cgctgcttac cgctggcttt cagtgcctca
    10921 agaaacggaa tggtatcttc acgcagtacg gcgcgcggtc ccatctcggt ggtcattgca
    10981 cagatatcca gacccagttg ctcactccag taatcaagac agtaccagtt tagcgtatgc
    11041 tgtacgtcgt gatattgctg gcgcatatat tccatcgctt cctgtggcgt aaccccgttt
    11101 ttcgcgcccc atgtttcagg caccagcttt tgccagaaat agttatcgaa ggcgaggtcg
    11161 agcaacgtgc cgtccatatc cagcagaacg gtatctacgt cctgccaggc aatgttgata
    11221 tgcatgaggg aaatctccag agtgaagcaa tttgcgcgac agggtagcat aacctgtcgc
    11281 gcaaacgtgt tattcgataa ggctttctga aggggtgatc agttgcgggt tcaggcagct
    11341 ttcataatag gcctgaatct ccatcatgcg agtgcgatga cgctggtaac gacgccaggc
    11401 ctgtacgcca ttgtaaatgg cgctacccag catcgtcagt agcagcaatg tggtgctgag
    11461 atagcgccac aggccggaac gatccgggat cggatgaagg ccaatatgct gcgtaccatt
    11521 ggcgtcagtg aagatttttg tgacgatacc ttcggcgtta aacggcgtat gcatcagcat
    11581 ttgtgccagt ttctggaaag cgttccactg ttcttgcggc gggtagtcgt aaagtgatgc
    11641 cgaaggccag ggctgatcaa caaaatcgct gccttcgtcg ctgacaatca ggaatccgcc
    11701 gggggccgga ctgtttagtg attgtgccgc tcgcgccgtt tcatgcgtga taaacggcgc
    11761 ggtggaggtt gccaccaggt tatccagcga ttccgcactc accgggcgta ataacacatt
    11821 cacgccatcg agcttcccgg cgttggcgcg ttttaccagc gcgtcccagt ctttactgtt
    11881 gccgagattg accagcgcat ttttcagtcg cacacagtca tctttggcag aacataaatc
    11941 cgctgtcttc agtacaatgt cgccaaaatc atcaagcaat accatgccgg atttttgaat
    12001 cgctgaacgt aatgaggcac tgacgcgaga ttcatcttcc ggtttagggt gcagctggcg
    12061 attaactgct tcagtcaatg ccgtcgcttt gttgaccagt tcagattctg gtaatggtaa
    12121 tgagcgggcg tcattccaga tgatctgcga gcagtcaaac ggtaaaaaag gtgaattggt
    12181 tttcgcgctc caggttccgg aagtgcgaat attacacatt cccgtaccgc taatacgcaa
    12241 tgtatcgcct acccgcacgc cagcgtcagc cagttgtttt acgctggtgg cttcaatggt
    12301 ctgcgcgcct ttcatccatg agagagtgaa tttcagcggc atatccagcg ggatccagaa
    12361 taacagcata aacagcacca gcagcgagcc cgcagcgata atcgtactgc gcagccagtg
    12421 ctgtaacgga aagtttttta cttcatcatg cagcgaaaga tatcgcccct gacgcactac
    12481 gtggcagtcg agatagatat caatatcggt ttgttgatcg agatcctgag caatgtatgg
    12541 ctgccagtgt gcgggataga ccaggtcgat aataccgagt gaaatattgt tgatctgttc
    12601 ctgatcgttt tcgccaaaca atccccaacg acggggtgta ccgcgcagac aatgaatttc
    12661 ccgcagggag gattttgccg ggggcgcgaa tagcccccac agacctgcgc ccagcagcag
    12721 taatgcgccg cctgccagcc acggaacaaa tacatccggg gtaatcaggc agaaaaagaa
    12781 catcaggaag gaggcgacga tcagcaacgc ttcacgcagc ccgcgcggac gactcagcgc
    12841 atattcttca tgcgtttctt tgcgaatatt gagtagctcg atttgctcac tctcttcccc
    12901 gcgaatcgac gcctgcgtgg acggaacagg ttgcaaggca tagccgcgcg tttcctgcat
    12961 gtactcctgt agcgtatgac cgttgagtga aataaccaac ggcagcgaat cggtatgaat
    13021 cagttcaacg gtattctcat cgttgatgta ctgttcccag aaggggggca ggtggacttc
    13081 taccgaatcg aggtagtaac gccatttatt cggatcgtcg gtagagatgc cgtaacgtgt
    13141 gatagcgtgt gtcagcatca tgacgttgtt gctttcggca ttcagcgcca gagagatcgg
    13201 tgccgcgcta gctcccgttg ggccaggcac ctgtaatacc tgcgtcaggc tctcaagata
    13261 attttcaacg gcgctacgtt cttcaggtgt gagtttacgc gtttgcgcat ccgcgaaggc
    13321 gttggtccag ggcagctgac tccgtctgga tcgcactttt atcagccatc ccgcaagtag
    13381 tgagcaggcc agcaaagcag ctaaaaaaat cacaatggtg ctcatgcttt ccccatctta
    13441 cttagtctgt caggcgtggt cagtcgcacc agacacaata catgattctg cggttggcta
    13501 tcggcaagcg tggagtttaa cttgagcatc attaagcaca tcacagtaac cgctgatcat
    13561 agcaaaccct ctgaaggcgg aatatcagca aattcctaga gttatttcaa cctattgact
    13621 tttaatttga tttgaggacg attatttatt aagttacata aatgtaaata acatgcaatt
    13681 cacattgccg aaagcgtgtc cgatatcgca caataaccgc tgatttcaca gcatagatcc
    13741 cacgatgagc aaatcattac aaaaacccac cattctgaat gttgaaactg tagcccgttc
    13801 ccgactgttt accgtcgaga gcgtggatct ggagttcagc aatggcgtgc ggcgtgttta
    13861 tgaacgaatg cgtccaacca accgggaagc agtgatgatt gtgccgattg tggacgatca
    13921 cctgatcctg atccgcgaat acgcagtggg aacagaatcc tacgaattag gtttttcgaa
    13981 aggattaatt gatccgggtg aaagcgtcta cgaagccgct aaccgcgagc taaaagaaga
    14041 ggttggattt ggtgcgaacg atctgacttt tttgaagaag ctcagcatgg caccgtctta
    14101 cttttccagc aaaatgaata tcgtggtcgc gcaagatctc tacccggaat cgctggaagg
    14161 cgatgagcca gagccgctac cacaggtgcg ctggccgctg gcgcatatga tggatttgct
    14221 ggaagaccct gacttcaacg aagcgcgtaa tgtcagcgcg ctgttcctcg tgcgcgaatg
    14281 gttgaaaggg caggggcgag tgtaaatgcg tgaagtttgc ctgatgcaca acgcttatca
    14341 ggcttatggg gttcctgcaa tatattgatt tttcacgatt ttgttagccg ggtaacgcgg
    14401 tcacccgcat ccagcataaa caaatgcact ttgtcagcca actgaaaagg cgccgaagcg
    14461 cctttttaat cagaacaatt cctgtgcctc accattatcg ataatggtcg ttcccacctc
    14521 gtgcaccgcc tgttgtgtcg gctgcgtacc ttcgatgaaa tactcttcgc ggctgttgcc
    14581 accattagct aactgcccgg tgctgcgatc gatattcacc gtcacaatac ccggtggcgg
    14641 cgtcagcggc tgctccggca caccttcaag aacggctttc atataagcgt cccatgcagg
    14701 ctgggcactc ttggctccgc cttcgtaacc tgagatctga tctttaatcg ctccggaagc
    14761 cgttgtatga ccgagattac gacggtgatc atcaaagcca atccagaccg aggtcacaac
    14821 gcccggaccg taacccgaga accacgcatc tttcgaactg ttagtggtac cggttttccc
    14881 gccgatatcg cgacgctgca aatcacgacc tgcacgccag ccagtaccct gccagcctgg
    14941 ctcaccaaag atattggtgt tcaaagcact cttaatcagg aatgccagcg gagtgttgat
    15001 gacgtgcggt gcgtactcct gcgcgccagt cttcgccact aacgcctgat ttgcctgctc
    15061 cagctgcggc attggtacag aaacattctg ctgctcgcgg gagatagcga catcttcaac
    15121 atcgttattt tccagcacgt tcgatttctg cgtatcaccg taaatcaccg gaatatcgca
    15181 ttccgggcag gctactttcg gtttcgcttc gaaaatcacg ccgccctgat cgttttcaat
    15241 tttgctgata aaccacgggt ccaccaggaa gccgccgttc gccatgaccg cgtagccgcg
    15301 cgccacctgc attggggtga aggacgctga acccagcgcc agcgattcgg tgtggacaat
    15361 gttttgcgcc gggaagccga agcgttgcag atattctgca gcgtagtcga cgcccatagc
    15421 ccgcattgca cgtaccatca ccacgttttt cgactgaccc agcccctgac gtaagcgaat
    15481 tggaccagca tattgtggcg gcgagttttt cggctgccag tcagaaccgg cacctgcgtc
    15541 ccagcgagaa attggcacat cgttcaacat acttgccagc gtcagacctt tatccatcgc
    15601 cgcggtgtag aggaacggtt tgatgttgga acccacctga cgcagtgcct gggtggcgcg
    15661 gttaaacttg ctctgattga aatcaaagcc accgaccagc gccataacgg caccgttttg
    15721 cggattgatc gacaccagcg ccgagttcac ttccggcact tgtgccagcc accatgcatc
    15781 gccaacctga cgaacccaga tttgctgacc cgtttgcaga acatcggtca ctttacgcgg
    15841 cgtcggtccc tgctgagtat ccgaacggta aggacgcgcc cagcgaacgc cttccatact
    15901 caatgcgacg gtcgacccgt ccgccagcat cgccgtcgct ccctgaggat tggcgctggt
    15961 gactgcggca ggcagcagcg gaccataggt cggcagcgcc ttcagcgtat cggtaatctt
    16021 gttgttatcc cacgccgact cgcccacttt ccacagcaca tttgccgggc cgcgatagcc
    16081 gtggcgcatg tcgtagtcca gcacgttatt acgtaccgcc tgctgtgcgg cctgctgcac
    16141 tttgcgggtg atggtggtgt aaatgcgata accgtcttca taggcacttt cgccataacg
    16201 gttatacatc tcctggcgca ccatttcgct caggtacggc gcagagaaag caatctccgg
    16261 cgcgtgatag ttagcgttaa tcgcctcagt gcgtgtctga tcgaactgtt gttgggtgat
    16321 atacccttca tccagcatcc gcgacagtac gacgttacgc cgcgcgacgg cacgatccat
    16381 tgagtagagc ggattaaagg tggaaggcgc tttcggcagc ccggctatca cagccatttc
    16441 gttcagcgtc agttggtcga ccgttttacc gaaatagact tgtgccgcag caccgacacc
    16501 ataggcgcgg taaccgaggt aaatcttatt cagataaagc tcgaggatct cgtctttcgt
    16561 cagcaactgt tcaatgcgaa tcgcgaggaa gacttcctta atcttacgca tcagcgtgcg
    16621 ttctggactg aggaagaagt ttctcgccag ctgctgggta atggtactcg ccccttgtga
    16681 cgcgtggccg gagaacagcg ccacacttgc cgcacggaag atccccaccg ggtcaacgcc
    16741 gtgatgctcg tagaagcggc tgtcttccgt cgcgataaag gctttcacca tctccggcgg
    16801 gatttgatcc aacgtaaccg gaatacgacg tttctcaccg tattgagcaa tcagctcgcc
    16861 atcggcgctg taaatctgca tcggaatttg caggcgaaca tcttttaatg tcgccacatc
    16921 cggcagttgt ggctcgatgt agcggtatag gccataaatc gagcctgctc ccagcagaat
    16981 gcaacagact gcaaggatca aaaaatactt tacgaacttc actggaaatt tcccatttag
    17041 tttcatttgg gcagtttata aacaaacgcg cggtagtata aaggcaagcc agacgcattg
    17101 atatacccgt cagagtgacg ggtgataagg agatcatcac aatggcattt aagatctggc
    17161 aaattggttt gcatttacaa cagcaagaag cggtagcggt tgcgatcgtg cggggcgcaa
    17221 aagaatgctt tttgcaacgc tggtggcggt tgccgctgga gaacgacatt atcaaagatg
    17281 ggcggattgt tgatgcgcag cagctggcta aaacgttgtt accttggagt cgcgaactgc
    17341 cgcagcgtca tcacattatg ttggcgtttc ccgccagtcg cacattacag cggtcatttc
    17401 cgcgcccgtc gatgtccctt ggtgagcggg agcaaacggc ctggctgtca gggacgatgg
    17461 cccgcgagct ggatatggat ccggactccc tgcgcttcga ttacagcgaa gactcactca
    17521 gtcccgccta taacgtgact gccgcgcaaa gcaaagagct ggcaacgctg cttactctgg
    17581 cagaaaggtt gcgtgttcat gttagtgcga tcaccccgga tgccagtgca ttacagcgat
    17641 tcctgccttt tttaccttct catcagcaat gtctggcctg gcgtgataac gaacagtggc
    17701 tgtgggcgac gcgctatagc tgggggcgca aactggcggt ggggatgact agcgcgaagg
    17761 agctggcggc agcgttattc gttgatcccg acagcgtcgc gatatgtggc gaaggcggat
    17821 ttgacccctg ggaggccgtt tctgttcgtc agccgctgct accgccgcct ggtggagact
    17881 ttgccatcgc gctggggctg gcgcttggga aggcgtactg atgaacccgc caattaattt
    17941 tttgccctgg cgacagcaac gccggaccgc ttttctgcgt ttctggttgc tgatgttcgt
    18001 tgcgcctctg ctgctggccg tcgggataac gctaatactg cgtctgacag gcagcgccga
    18061 agcgcgcata gacgcagttt tgcttcaggc ggaacaacaa ctcgcccgca gcttacagat
    18121 aacgaagcca cgtttgctgg agcaacagca gttacgcaaa cagcgttcgc aacggcagcg
    18181 ccagcgacaa tttacccgcg actggcaatc tgcgctggaa gcactggcgg cgcttttacc
    18241 cgagcacgcc tggctgacaa cgataagctg gcagcaggga acgctggaga tcaaggggct
    18301 gacaacaagc attaccgcgt taaacgcact agaaacgtca ctccgccagg atgcgtcttt
    18361 tcatctcaat cagcggggag ccacgcagca ggatgcgcag ggacgctggc aatttgagta
    18421 tcagttaaca aggaaggtta gcgatgaaca tgttctttga ctggtggttc gccacatcac
    18481 cccgcctccg ccagttttgc tgggcattct ggttgctgat gttagttacg ctcatttttc
    18541 tgtcatcgac acaccatgaa gagcgcgacg cattaattcg attacgggca agtcatcacc
    18601 agcagtgggc cacactgtat cgcctggttg acaccactcc cttcagcgaa gaaaaaacgc
    18661 tgcccttttc gccactggat tttcagttgc ccggcgcgca actggtttcc tggcatccat
    18721 ccgcgcaggg aggtgagttg gcgttgaaaa cgctgtggga agcagtgccg tcggcattca
    18781 cacggctggc agagcgcaac gtcagcgtga gccgtttttc gttaagcgtg gaaggtgatg
    18841 atcttttgtt cacgctacaa ctggagacgc cgcatgaggg ttaaacgctg gttgttggca
    18901 ggtattgcat tgtgcctttt aaccggtatg cgtgaccctt ttaaaccgcc ggaagatcta
    18961 tgccggatta gcgaacttag ccagtggcgc tatcagggga tggtagggcg aggcgagcgc
    19021 atcatcggtg taataaaaga cgggcaaaag aaatggcgac gggtgcagca aaacgatgtg
    19081 ctggaaaacg gctggacaat tttacagctg acgccagacg tactaacgct gggtaccggg
    19141 acaaactgcg aaccgccaca atggttgtgg caacggcaag gagatacaaa tgaagcaatg
    19201 gatagccgca ctactgttga tgctgatacc cggcgtacag gcggcaaagc cgcaaaaagt
    19261 gacgctgatg gtggatgacg ttccggtagc tcaggtgttg caggcgctgg ctgaacagga
    19321 gaagttgaac ctggtcgtgt cgccagacgt cagcggtacg gtgtcgttac atctaacaga
    19381 tgttccctgg aagcaggcac tacaaactgt agtgaaaagc gccggactga taacgcggca
    19441 ggaaggcaac attctctcag tgcattccat tgcctggcag aataacaata tcgcccgcca
    19501 ggaggcggag caggcgcggg cgcaggcaaa tctgccgctg gaaaatcgca gtataaccct
    19561 gcaatacgcc gacgcgggag aactggcgaa agcgggggag aagctactga gtgccaaagg
    19621 gagtatgacc gtcgataaac gcaccaatcg ccttttgcta cgagataaca aaacggcgtt
    19681 aagcgcgctt gaacagtggg tagcgcaaat ggatctgccg gtcgggcagg ttgagctgtc
    19741 ggcgcatatt gtcaccatta atgaaaaaag tttgcgtgag ttaggcgtga aatggacgct
    19801 ggccgatgcg caacacgctg gtggcgttgg gcaagtcacc acgcttggta gcgacctctc
    19861 cgtagcgacg gcgacaacgc atgtcggttt taacattggg cgcatcaacg gacgcttgct
    19921 ggatcttgag ctttccgcgc tcgaacaaaa acagcagctg gatattatcg ccagtccgcg
    19981 tctgctggcc tcacatcttc agcctgccag cattaaacag gggagcgaaa ttccatatca
    20041 ggtttccagc ggggaaagtg gcgcgacgtc ggtggaattt aaagaggccg tcctggggat
    20101 agaggtcacg cccacggtgt tacaaaaagg tcgcatccgg ctgaaattac acatcagcca
    20161 gaacgttccg gggcaggtgc tacagcaggc cgatggcgaa gtgctggcga ttgataagca
    20221 ggagatcgaa acgcaggtcg aggtcaaaag cggagaaacg ttggcgctgg gcggcatttt
    20281 tacccgtaaa aataaatcgg gtcaggatag cgtaccgttg cttggcgaca ttccctggtt
    20341 cgggcaatta tttcgtcatg acggaaaaga atatgaacga cgcgagttag tggtgtttat
    20401 cacgccacga ctggtttcca gtgagtaaac agccgtaaaa gcggtaatgt ttttatgctg
    20461 aacgtgtttc atctatttga cgcgcgcagg tatttagcat acaaggagta ccgatttgag
    20521 agttggcgct cttcgctgcc tgcgttccat gatgatgatt tatcattcag gcggcatttt
    20581 gctgtctttt ttacgctaat cttacccggt gatttatcgc cagagcggtg gtagcaaggc
    20641 agcgcgcttg cagcgaccag atatgcagag ggatgggtga tttattcagt tgccaaaccc
    20701 gctggagtat tgagataatt ttcagtctga ctctcgcaat atcttatgag gtttcagttc
    20761 atgtcctgcg gcgctctctg agcgaagcgg gtttatcatt aacgaatagt cttagtagta
    20821 ccgaaaaaat ggcagagaaa cgcaatatct ttctggttgg gcctatgggt gccggaaaaa
    20881 gcactattgg gcgccagtta gctcaacaac tcaatatgga attttacgat tccgatcaag
    20941 agattgagaa acgaaccgga gctgatgtgg gctgggtttt cgatttagaa ggcgaagaag
    21001 gcttccgcga tcgcgaagaa aaggtcatca atgagttgac cgagaaacag ggtattgtgc
    21061 tggctactgg cggcggctct gtgaaatccc gtgaaacgcg taaccgtctt tccgctcgtg
    21121 gcgttgtcgt ttatcttgaa acgaccatcg aaaagcaact tgcacgcacg cagcgtgata
    21181 aaaaacgccc gttgctgcac gttgaaacac cgccgcgtga agttctggaa gcgttggcca
    21241 atgaacgcaa tccgctgtat gaagagattg ccgacgtgac cattcgtact gatgatcaaa
    21301 gcgctaaagt ggttgcaaac cagattattc acatgctgga aagcaactaa ttctggcttt
    21361 atatacactc gtctgcgggt acagtaatta aggtggatgt cgcgttatgg agaggattgt
    21421 cgttactctc ggggaacgta gttacccaat taccatcgca tctggtttgt ttaatgaacc
    21481 agcttcattc ttaccgctga aatcgggcga gcaggtcatg ttggtcacca acgaaaccct
    21541 ggctcctctg tatctcgata aggtccgcgg cgtacttgaa caggcgggtg ttaacgtcga
    21601 tagcgttatc ctccctgacg gcgagcagta taaaagcctg gctgtactcg ataccgtctt
    21661 tacggcgttg ttacaaaagc cgcatggtcg cgatactacg ctggtggcgc ttggcggcgg
    21721 cgtagtgggc gatctgaccg gcttcgcggc ggcgagttat cagcgcggtg ttcgtttcat
    21781 tcaagtcccg acgacgttac tgtcgcaggt cgattcctcc gttggcggca aaactgcggt
    21841 caaccatccc ctcggtaaaa acatgattgg cgcgttctac cagcctgctt cagtggtggt
    21901 ggatctcgac tgtctgaaaa cgcttccccc gcgtgagtta gcgtcggggc tggcagaagt
    21961 catcaaatac ggcattattc ttgacggtgc gttttttaac tggctggaag agaatctgga
    22021 tgcgttgttg cgtctggacg gtccggcaat ggcgtactgt attcgccgtt gttgtgaact
    22081 gaaggcagaa gttgtcgccg ccgacgagcg cgaaaccggg ttacgtgctt tactgaatct
    22141 gggacacacc tttggtcatg ccattgaagc tgaaatgggg tatggcaatt ggttacatgg
    22201 tgaagcggtc gctgcgggta tggtgatggc ggcgcggacg tcggaacgtc tcgggcagtt
    22261 tagttctgcc gaaacgcagc gtattataac cctgctcaag cgggctgggt taccggtcaa
    22321 tgggccgcgc gaaatgtccg cgcaggcgta tttaccgcat atgctgcgtg acaagaaagt
    22381 ccttgcggga gagatacgct taattcttcc gttggcaatt ggtaagagtg aagttcgcag
    22441 cggcgtttcg cacgagcttg ttcttaacgc cattgccgat tgtcaatcag cgtaacaaca
    22501 agaaaggtca ggccgcttat caagcggtct attagcttca ggttaattgc aacgtggtaa
    22561 gcattaacct tttagtgggg tgttaaatgg atgaattcaa accagaagac gagctgaaac
    22621 ccgatcccag cgatcgtcgt actggtcgtt ctcgtcaatc ttctgaacgt tctgagcgta
    22681 ctgaacgtgg cgaaccgcag atcaattttg atgatattga acttgatgac actgacgatc
    22741 gccgtccgac tcgtgcgcaa aaagagcgca atgaggaacc ggaaatcgaa gaagaaattg
    22801 acgaatccga agatgaaacc gtggatgaag agcgcgtaga gcgtcgtccg cgtaagcgca
    22861 aaaaagcagc cagtaaaccc gcttctcgtc agtatatgat gatgggcgtc ggcattctgg
    22921 ttctactgct gttgatcatc ggtatcggtt ctgcgctaaa agccccctcg acctcttcca
    22981 gcgatcaaac cgcgtctggc gataagagta ttgatcttgc aggcaatgcg accgatcagg
    23041 cgaatggggt gcagccagcg ccgggaacca cgtctgcgga aaatactcag caggatgttt
    23101 ctctgccacc gatctcttct acgccgactc aagggcaaac cccggcggca acggatggtc
    23161 aacaacgtgt tgaagtgcag ggtgacctga acaatgcgct gactcagcca caaaatcagc
    23221 aacagttgaa caatgtggcg gtcaattcca cattgccgac cgaacccgcg actgtcgcgc
    23281 ctgttcgcaa tggcaatgca tcgcgtgaca cggcgaaaac gcaaaccgct gaacgtccgt
    23341 ccactacgcg cccagctcgt cagcaggcgg tgattgaacc gaaaaaaccg caagcaaccg
    23401 tgaaaacgga gccgaagccg gtagcacaga cgccgaagcg tactgaacca gctgcccctg
    23461 tggcgagcac gaaggcaccg gctgcgactt ctacgccagc accaaaagag acggcgacta
    23521 cggctccagt acagacggca tccccggcgc aaaccacggc aacaccagcc gctggaggga
    23581 agaccgcagg taatgttggt tcgttgaaat cggcaccgtc cagccattac actctgcagc
    23641 tgagcagttc ctctaactac gacaacctga acggttgggc gaagaaagag aatctgaaaa
    23701 actacgttgt ctatgaaacg acgcgtaatg gtcagccgtg gtatgtcctg gtttctggcg
    23761 tgtacgcttc gaaagaagag gcgaaaaaag cggtatctac attgccagca gatgttcagg
    23821 ccaaaaaccc gtgggcgaaa ccgctgcgtc aggtacaggc cgatctgaag taatcaaggt
    23881 tatctcccgc aatggtttat cgttgcggga gctgcctgaa gcgctggatg ctgtcggagc
    23941 tttctccaca gccggagaag gtgtaattag ttagtcagca tgaagaaaaa tcgcgctttt
    24001 ttgaagtggg cagggggcaa gtatcccctg cttgatgata ttaaacggca tttgcccaag
    24061 ggcgaatgtc tggttgagcc ttttgtaggt gccgggtcgg tgtttctcaa caccgacttt
    24121 tctcgttata tccttgccga tatcaatagc gacctgatca gtctctataa cattgtgaag
    24181 atgcgtactg atgagtacgt acaggccgca cgcgagctgt ttgttcccga aacaaattgc
    24241 gccgaggttt actatcagtt ccgcgaagag ttcaacaaaa gccaggatcc gttccgtcgg
    24301 gcggtactgt ttttatattt gaaccgctac ggttacaacg gcctgtgtcg ttacaatctg
    24361 cgcggtgagt ttaacgtgcc gtttggccgc tacaaaaaac cctatttccc ggaagcagag
    24421 ttgtatcact tcgctgaaaa agcgcagaat gcctttttct attgtgagtc ttacgccgat
    24481 agcatggcgc gcgcagatga tgcatccgtc gtctattgcg atccgcctta tgcaccgctg
    24541 tctgcgaccg ccaactttac ggcgtatcac acaaacagtt ttacgcttga acaacaagcg
    24601 catctggcgg agatcgccga aggtctggtt gagcgccata ttccagtgct gatctccaat
    24661 cacgatacga tgttaacgcg tgagtggtat cagcgcgcaa aattgcatgt cgtcaaagtt
    24721 cgacgcagta taagcagcaa cggcggcaca cgtaaaaagg tggacgaact gctggctttg
    24781 tacaaaccag gagtcgtttc acccgcgaaa aaataattct caaggagaag cggatgaaac
    24841 agtatttgat tgccccctca attctgtcgg ctgattttgc ccgcctgggt gaagataccg
    24901 caaaagccct ggcagctggc gctgatgtcg tgcattttga cgtcatggat aaccactatg
    24961 ttcccaatct gacgattggg ccaatggtgc tgaaatcctt gcgtaactat ggcattaccg
    25021 cccctatcga tgtacatctg atggtgaaac ccgtcgatcg cattgtgcct gatttcgctg
    25081 ccgctggtgc cagcatcatt acctttcatc cagaagcctc cgagcatgtt gaccgcacgc
    25141 tgcaactgat taaagaaaat ggctgtaaag cgggtctggt atttaacccg gcgacacctc
    25201 tgagctatct ggattacgtg atggataagc tggatgtgat cctgctgatg tctgttaacc
    25261 caggttttgg cggtcagtcg ttcattccac aaacactgga taaactgcgc gaagtacgtc
    25321 gtcgtatcga cgagtctggt tttgacattc gactggaagt ggacggtggc gtgaaggtga
    25381 acaacattgg cgaaatcgct gcggcgggcg cggatatgtt cgtcgccggt tcggcaatct
    25441 tcgaccagcc agactacaaa aaagtcattg atgaaatgcg cagtgaactg gcaaaggtaa
    25501 gtcatgaata agtttgaaga tattcgcggc gtcgcttttg atctcgacgg tacgctggtt
    25561 gacagtgctc ccggtcttgc tgctgcggta gatatggcgc tgtatgcgct ggagttaccc
    25621 gtcgcaggtg aagaacgcgt tattacctgg attggtaacg gcgcagatgt tctgatggag
    25681 cgtgcattgg cctgggcgcg tcaggaacgt gcgactctgc gtaaaacaat gggtaaaccg
    25741 cccgttgatg acgacattcc ggcagaagaa caggtacgta ttctgcgtaa actgttcgat
    25801 cgctactata gcgaggttgc cgaagagggg acgtttttgt tcccgcacgt tgccgatacg
    25861 ttgggcgcgt tgcaggctaa aggcctgccg ctaggcctgg tcaccaacaa accgacgccg
    25921 ttcgtcgcgc cactgctcga agccttagat attgccaaat actttagcgt ggttatcggc
    25981 ggcgatgatg tgcaaaacaa aaaaccgcat ccggacccgc tgttactggt ggctgagcgg
    26041 atgggaattg ccccacaaca gatgctgttt gtcggcgact cacgcaatga tattcaggcg
    26101 gcaaaagcgg caggttgccc atcagttggc ttaacctacg gatataacta cggcgaggct
    26161 atcgatctca gccagcctga tgtaatttat cagtctataa atgaccttct gcccgcatta
    26221 gggcttccgc atagcgaaaa tcaggaatcg aaaaatgact aagcccatcg tttttagtgg
    26281 cgcacagccc tcaggtgaat tgaccattgg taactacatg ggtgcgctgc gtcagtgggt
    26341 aaacatgcag gatgactacc attgcattta ctgtatcgtt gaccaacacg cgatcaccgt
    26401 gcgccaggat gcacagaagc tgcgtaaagc gacgctggat acgctggcct tgtatctggc
    26461 ttgtggtatc gatcctgaga aaagcaccat ttttgttcag tcccacgtac cagaacatgc
    26521 gcagttaggc tgggcactga actgctatac ctacttcggc gaactgagcc gcatgaccca
    26581 gtttaaagat aaatctgcgc gttatgccga gaacatcaac gctggtctgt ttgactatcc
    26641 ggtgctgatg gctgcggaca tcctgctgta tcaaactaat ctggtaccgg tgggtgaaga
    26701 ccagaaacag cacctggaac tgagtcgcga tatcgcccag cgtttcaacg cgctgtatgg
    26761 cgagatcttt aaggtgccgg agccgtttat tccgaaatct ggcgcgcgcg taatgtcgct
    26821 gctggagccg accaagaaga tgtccaagtc tgacgataac cgcaataacg ttatcggcct
    26881 gctggaagat ccgaaatcgg tagtgaagaa aatcaaacgc gcggtcactg actccgacga
    26941 gccgccggta gttcgctacg atgtgcagaa caaagcgggc gtttccaacc tgctggatat
    27001 cctttctgcg gtaacgggcc agagcatccc agaactggaa aaacagttcg aaggcaagat
    27061 gtatggccat ctgaaaggtg aagtggctga tgccgtttcc ggtatgctga ctgaattgca
    27121 ggaacgctat caccgtttcc gcaacgatga agccttcctg caacaggtga tgaaagacgg
    27181 cgcagaaaaa gccagtgtac acgcttcccg tacgctgaaa gcggtgtacg aagcgattgg
    27241 ttttgtggcg aagccgtaat atcaaaacgc tcggacggtc ccctcgcccc tttggggaga
    27301 gggttagggt gaggggagca ggcaagcgcc agtgggaatt accctttgct cgcattgtcg
    27361 aataaatcac ttcgtcatca ctctgaaacc ggggaaaccc ggttttttta tcctcatttg
    27421 caataatccg aaaaaatgtg aagcgcctcg ccgtttccac atctcacagt tgccacttat
    27481 cttcatttta atgaagatat aaacattcat tttattgaaa atatattatg cgtcgaacgt
    27541 ttatcaaaaa agaaggcgtc gtcatcacga cgctggcccg ttatttgttg ggtgaaaaat
    27601 gcggtaatcg actgaaaacc atagatgagc tggcaactga atgccgttca tcggttggcc
    27661 tgacgcaggc cgcgttgaaa acgttggaat caagtggagc gatacggatt gaacgccgtg
    27721 ggcgcaatgg cagttatctg gtcgaaatgg ataacaaagc gctgctgagt catgtggata
    27781 tcaataacgt tgtgtgtgcc atgcctttgc cctatacccg cttgtacgaa ggtttagcaa
    27841 gcgggctgaa agcccagttt gatggcattc ctttttatta tgcgcatatg cgtggcgcgg
    27901 atattcgtgt ggagtgtttg cttaacggtg tctatgacat ggcagtagtt tcccgcctgg
    27961 cggcggaaag ttatctcagc caaaacaatt tgtgcatcgc cctcgagctt ggcccgcata
    28021 cgtatgttgg tgaacatcaa ctgatatgcc gcaaaggtga gtctggaaat gttaagcgag
    28081 taggtctcga tagtcgttcg gcggatcaga aaatcatgac cgatgtcttt tttggcgata
    28141 gtgatgtgga acgtgtcgat ctctcttatc acgagagttt acaacgcatt gttaaaggcg
    28201 atgtcgatgc ggttatctgg aacgtggtgg cggaaaacga actgaccatg ctgggattag
    28261 aggcgacgcc gctcacagac gatccgcgat ttttacaggc caccgaagcc gtggtcctga
    28321 cgcgagtcga tgattaccca atgcaacaac tgctgcgtgc cgttgtagat aaacacgccc
    28381 tgctcgccca tcaacaacgg gtagtgagtg gggaacagga accgagttat taaacgaaag
    28441 gcatctgata tggaaaccag actcaacctg ctttgcgagg caggcgttat tgataaggac
    28501 atctgcaaag gcatgatgca ggtcgtcaac gtactggaaa cagagtgcca tctgccggtg
    28561 cgcagtgagc aaggaacgat ggcgatgaca catatggcga gtgcactgat gcgcagtcgc
    28621 cgtggtgaag aaatagagcc gctggataac gaattgctgg cagagctggc gcaatccagc
    28681 cactggcaag ccgttgtgca attgcatcag gtgttgttga aggaattcgc actggaagtt
    28741 aatccgtgtg aagaaggcta tttgctggcc aacctttatg gattatggat ggctgctaac
    28801 gaagaggttt gaagctcccg acaaatccac gtcgcacaac ctaaatattc aaaaaaatga
    28861 atatttaatt caaggaacag aaacgaggca acaagatgtt tgtagaagca ttgaaacgcc
    28921 agaacccggc gctgatttcc gccgcactaa gtttgtggca gcagggcaag atcgccccgg
    28981 acagctgggt gatcgacgtg gatcaggtac tggaaaacgg taagcgactg attgagacgg
    29041 cgcggcttta cggcattgaa ctgtatctga tgaccaagca gtttggccgt aacccatggc
    29101 tggcggaaaa attgctggca ttaggttaca gcggcattgt ggcggtggat tacaaagagg
    29161 cgcgagtcat gcgccgcgct ggtttgtcgg tggcgcatca ggggcatctg gtacaaatcc
    29221 cttgtcatca ggttgctgac gccgttgaac agggcaccga cgtcatcacc gtgtttactc
    29281 tcgacaaagc gcgggaagtt tctgcggcag cggtgaaggc cggacgtgtg cagtttgtgc
    29341 tgctcaaagt ttatagcgac gatgattttc tttatccggg gcaggagagc ggttttgtac
    29401 agcattcgct gcacgaggtg gttgctgaaa ttaagaaact gccgggtctg catttagccg
    29461 gacttaccca tttcccttgc ctgctttggg atgaggctgc cggaaaagtt ttgccgacac
    29521 cgaatcttca cacgctgata caggcacggg atcaactggc gaaatctggt attgcacttg
    29581 agcaactgaa cgcgccttca gcgaccagct gcacttcgct gccattactg gcgcaatacg
    29641 gtgtgactca tgccgaaccc ggtcatgcgc tgacgggcac tattccggca aaccagcagg
    29701 gcgatcagtc tgaacgtatc gcgatgctct ggttgagtga aatctcccat catttccgtg
    29761 gcgacagcta ctgctacggc ggcggttact accgtcgtgg tcatgcgcaa catgcgttgg
    29821 tgtttacgcc agaaaatcaa aagattactg aaaccaatct caaaactgtg gatgacagca
    29881 gtatcgacta caccctgtcg ctggcaggcg agtttccggt aagcagtgca gtggtgctct
    29941 gtttccgcac gcagattttt gtcacccgta gcgatgtggt gctggtgtcc ggtattcacc
    30001 gtggcgaacc gaaaatcgtt ggtcgttatg acagtcttgg aaactctctg ggggcgtaat
    30061 ggcgcgattt gtggtgttag tgattgatag ctttggcgta ggggcaatga aagatgtcac
    30121 gctggtgcgt ccgcaagatg cgggagcgaa tacatgtggt cacatcctga gccagttgcc
    30181 gcatttgcag ctaccaacgc tggagaagct ggggctaatc aacgcattgg gttatgcgcc
    30241 aggcgatatg cagccgtcag attccgcaac ctggggcgtg gcagagctgc aacatgaagg
    30301 tggcgatacc tttatggggc atcaggaaat tttaggcacg cgcccgttac cgccgctgcg
    30361 gatgcctttt tgcgatgtga ttgaccgtgt tgagcaggca ttagtatccg ccggttggca
    30421 ggtggagcgc cgtggcgatg aactgcaatt tctgtgggtc aatcaggcgg ttgcgattgg
    30481 cgataatctc gaggcggatt taggccaagt ctataacatt accgccaatc tctctgtgat
    30541 ctcttttgac gacgcaatca aaattggtcg tatcgtgcgt gagcaggtac aggtcggtcg
    30601 ggtcattaca tttggtggcc tgttaaccga cagtcaacgc attctcgatg ccgcagaaag
    30661 caaagaaggg cgctttattg gtatcaatgc gccgcgttct ggcgcttatg acaacggctt
    30721 ccaggtcgtg catatgggct acggtgtcga taaaaaagtg caggtgccgc aaaaactgta
    30781 tgaggcgggc gtgccaaccg tgctggtggg taaggtggca gatatcgtca gcaatcctta
    30841 tggcgtgagc tggcaaaacc tggtggatag ccagcggatt atggatatca ccctcgacga
    30901 atttaacacc catccgacgg cgtttatttg caccaatatc caggaaaccg acctcgctgg
    30961 tcatgcagaa gacgtcgcac gttatgccga acgtttgcag gtcgttgacc gtaaccttgc
    31021 ccggcttgtt gatgcgatgc agccagatga ttgcctggtc gtgatggcgg atcacggcaa
    31081 cgatccgacc attggtcaca gccaccatac ccgcgaagtg gtgccgctgc tggtttatca
    31141 gcaaggcctg gctgctacgc agctcggtgt gcgcaccacg ctttctgatg tgggagctac
    31201 cgtgtgtgaa tttttccgtg cgccaccgcc acaaaatggt cgctcttttc tttcctccct
    31261 ccggtttgca ggagacaccc tatgagtttt gatccgacgg gttacaccct ggcccatgag
    31321 catctgcata ttgatctctc cggctttaaa aacaacgtgg actgccgcct tgatcagtat
    31381 gcgttcattt gccaggagat gaacgacctg atgacccggg gtgtacgtaa tgtgattgag
    31441 atgaccaacc gttacatggg gcgcaatgcg caatttatgc tcggtgtaat gcgcgaaacc
    31501 gggatcaacg tggtggcctg tactggttat taccaggatg cgtttttccc ggaacatgtg
    31561 gtgacccgca gcgtgcagga actggcgcag gagatggtcg atgaaattga acagggtatc
    31621 gatggcacgg agctgaaagc cgggctcatc gcggagatcg gctccagcga aggaaagatt
    31681 acgccgctgg aagagaaggt atttattgcc gctgcgctgg cgcataacca gaccggacgc
    31741 ccgatctcca cgcatacgtc gttcagcacc atggggctgg aacaactggc tctgttgcaa
    31801 gcccacggag ttgatctttc gcgcgtcacc gttggtcact gcgatctgaa agacaacctc
    31861 gacaacattt tgaagatgat cgatctcggc gcgtacgtgc agttcgacac cattggtaag
    31921 aacagttact acccggacga aaagcgcatt gcgatgctcc acgcgctacg tgaccgtggg
    31981 ttgctgaacc gcgtcatgct gtcgatggat attacgcgcc gctcccattt aaaagccaat
    32041 ggtggttatg gctatgacta tttattgacc acctttattc cgcaattgcg ccagtcagga
    32101 ttcagtcagg ccgatgtgga tgtgatgtta cgtgaaactc cctctcaatt tttccaataa
    32161 ggacagactc atgaaaaaga ttggcgttgc aggcttacag cgtgagcaga ttaaaaaaac
    32221 cattgaagcg acggctcctg gcagttttga agttttcatt cacaacgaca tggaagcggc
    32281 aatgaaagtg aaatccgggc aactggatta ctacatcggt gcgtgtaata ccggtgcggg
    32341 cgcggcattg tcgattgcca tcgcggtgat tggctataac aaaagttgca ccattgccaa
    32401 accaggcatt aaagcgaaag acgagcatat cgccaaaatg atcgctgaag gaaaagtggc
    32461 gtttggcctt tccgttgagc acgtcgaaca cgcgattccg atgctgatta accatctgaa
    32521 ataaaaggca cgactatgga tctgtatatt cagattatcg tggtggcgtg cctgacgggt
    32581 atgacatcgc ttctggcgca tcgctcggcg gctgtttttc atgacggcat ccgcccgatc
    32641 ctgccgcaac tgattgaagg ctatatgaac cgtcgcgagg cggggagtat cgcttttggt
    32701 ctgagcattg gttttgtggc ctcggtgggg atctctttta ccctgaaaac cgggctgctc
    32761 aacgcatggt tactctttct tcctaccgat atcctcggcg tactggcgat aaacagcctg
    32821 atggcgtttg gtcttggcgc tatctggggc gtgttgatcc ttacttgcct gttgccagta
    32881 aaccagctgc tgaccgcgct gccggtggat gtattaggta gcctcgggga attaagctcg
    32941 ccggtagttt ctgcttttgc actgttcccg ctggtggcga ttttctacca gtttggctgg
    33001 aagcaaagtc tggtcgccgc cgtggttgtt ctgatgaccc gtgtggtagt cgtgcgctat
    33061 ttcccacatc ttaaccctga atccatcgaa atctttattg gcatggtgat gctgctgggg
    33121 atcgcgataa ctcacgacct gcgtcatcgt gatgaaaatg atattgatgc cagcgggctt
    33181 tcggtgtttg aagaacgcac gtcgcggatt atcaaaaact taccctatat cgccatcgtg
    33241 ggagcattga ttgccgccgt tgccagcatg aagattttcg ccggcagtga agtgtcgatc
    33301 ttcactctgg agaaagcgta ctccgcaggc gtaacgccgg aacaatcgca aacgctgatc
    33361 aaccaggcgg cactggcaga atttatgcgc gggctgggtt ttgtaccgtt gattgccacc
    33421 accgccttag caacgggtgt gtatgcagtt gcgggcttta cctttgttta tgcggtgggc
    33481 tatctctcgc cgaatccgat ggttgcagcg gtattaggcg cagtggttat ttcggcggaa
    33541 gtcttgctgc ttcgttctat cggcaaatgg ctgggacgct acccgtcggt gcgtaatgcg
    33601 tcggataaca tccgtaacgc catgaatatg ctgatggaag tggcgctgct ggtcggttcg
    33661 attttcgcag caattaagat ggcgggttat accggattct ctatcgcggt tgccatttac
    33721 ttcctcaacg aatccctggg ccgtccggta cagaaaatgg cggcaccggt cgtggcagta
    33781 atgatcaccg gtattctgct gaatgttctt tactggcttg gcctgttcgt tccggcttaa
    33841 ggaaggcacc tatgaagacg tttcctctgc aaagcctgac gcttattgag gcgcagcaaa
    33901 agcagtttgc gctggtggac acgatttgcc gtcactttcc cggcgcggag tttctaacca
    33961 gcggtgattt gggcttaacg ccggggctga atcaaccgcg tattacccaa cgggtggagc
    34021 aggtgctggc tgatgcattt cacgcacagg ctgcggcgct ggtgcagggc gcggggactg
    34081 gcgcgattcg cgccgggctg gcagctttgc tcaaaccggg gcagcgtctt ctggtgcatg
    34141 acgcgcctgt ttacccgacg acacgggtta ttattgagca gatggggctg acgcttatta
    34201 ctgttgattt caatgacctg tcggcactga agcaggtcgt cgacgagcaa caaccggatg
    34261 cggcgctggt gcagcatacg cgccagcagc cgcaggacag ctacgtgctg gcagatgtgc
    34321 tggcaacgtt gcgcgcggca ggtgttccag cgttaaccga tgacaactat gcggtgatga
    34381 aggtggcgcg aatcggctgt gaatgcggcg cgaatgtctc gacattttcc tgcttcaagc
    34441 tatttgggcc agagggtgtt ggtgcagtgg tcggcgatgc tgatgttatc aaccgtattc
    34501 gcgccacgct ttactccggc ggtagccaga tccagggcgc acaggcgctg gaagtattgc
    34561 gttgtctggt gtttgcgcca gtgatgcacg cggtgcaggc aggggtatct gaacggttgc
    34621 tggctttgct taacggtggt gaggtgccgg aagtgaaaag cgcggtgatt gctaatgcgc
    34681 agtcgaaggt gttgattgtc gagtttcatc agccgattgc cgccagagtg ctggaagagg
    34741 cgcaaaagcg cggtgccttg ccttacccgg tgggtgcaga gtcgaaatat gaaatcccgc
    34801 cgctctttta tcgcctttcc ggaacgtttc gccaggcgaa tccacaatca gaacattgtg
    34861 cgattcgcat taacccgaat cgcagcggtg aagagacggt gctgcggatt ttgcgtgaga
    34921 gtattgccag tatttaatgc tggatagcac cgtcaagcta aattccgtac tgaacggtcc
    34981 cctcgcccct ttggggagag ggttagggtg aggggaacgt acgggatgta aaattcaacg
    35041 cgccagcatc gccggatgcg atgctggcgc atcttatccg gcctacggtt aatgtctttt
    35101 attatcaata gttaaggtga tgcgattggc gcggcagaag agttcggaga cgtgcaccgg
    35161 tttgtcattc tgatcaaagg cgattttgct gatgcgaaac aacggttcgc ccagttcgca
    35221 tttcagccat tttgcctgcg ggcgggtggc ggcaaagata tcgatggtct ttttgtcgct
    35281 gaccactcgc gtgtcaaaac gctcctgaaa taactgatag gtggagcttc cttcgacgta
    35341 aatctcatca aagtcaggat aacgcgacag cgggatccat gaactatcaa taaacagcgg
    35401 ctctttatcg agatacatca cccggcagag atggaacact tcgctgtttc cggggatgtt
    35461 cagctttttg caaaacggcg cggcgctgac ccgttcctgt tcgatcactt tctctttcgt
    35521 cgccttgcct tgtgagacgc caaaatcggt aaaaccactg acagtaagca gggcgttttc
    35581 aactttctgg ctttgtacaa aggtgccttt tccctgccag cggatcagta cgccgtccgc
    35641 cactaagtcg ctgatggctt tgcgaatggt aatgcggctg acgttatatt gtgtacaaag
    35701 ctcgttttcg gtagggatct gttgcccggc ctggtaaacc ccctgcgcga tatcatccag
    35761 cagtcgctgg cggacggtag cgtagaggag ttgatgagag tagcggtccg tagctgacat
    35821 aatcgaccct gaaataaaga atgaattact cggattatag ggtcgttttc cggcaagtaa
    35881 cagcacgttg gcgtgaagat gctcacgcca acgttatacc taccaggcac cgtggtactg
    35941 aatggttttc gccgcgcacg ccgttccctg cgccatcgcc tgcggtaatg tcatccccgc
    36001 agaccagccg caaaggaatc cggcaatgaa cgaatctccg gcacccatgg tgtcgataac
    36061 cgtcaccggt tcaggagcct gacgccagaa ctgcgcgcca tcccaggcaa tgctgccgtt
    36121 ttcacccagc gtgacaatca ctgttcctgc gccacgggca acaatcgctt tcatcttcag
    36181 acgcagcgtt tcgtcttctt gcggtgcgga ggcaaaggca aaatcgagat gcggcaccag
    36241 tgtctgccag agcgggctgt cccacttgtc ggagaagtcg aaagcggtaa gtttgcccgc
    36301 agcgtgcagc tgtgggaatg cgtcttccgc atgtccccag attgccgcgt gcacaatgtc
    36361 atactgcgcc agccaggcgt aatcctcttc actcagggca aagtcggcca tcacgccttc
    36421 ggtgtagtcg ccaaaaacgc gatcattgtc gtgcagttcc acctgagttt gtgcggtaac
    36481 gccgtgtttc gtatggacat ggctgatatc gacgcccatg cgggcgagat cctgcttcag
    36541 ctttgtgccg tagtcatcgt cacccaccca ggtaatgcat cccggctgta tgccgtagcg
    36601 agtgcagtac accgccacat tgaccgcatt accgccagaa aacgctttat tcagttgcgg
    36661 gtagatatcg acgcagttat cgccgattgt cgccagggtt ttcatctctc atcctccggc
    36721 agcagcgcgc gaaagcgttc cagcgcctgg cgggcataga gtctgggctc gttcatatac
    36781 atcgtcacca gctccaccgt acagtaaccc tcatagcccc gctcaataat atcgcgcatc
    36841 agttcccgca gcggcatttt gccttctcca ggaatgtaat gcgtgtcgct ggccccgtcg
    36901 ctgtcgacaa tatgcagatg acgtaattta tcgcccagtt tgtcgaaata actcatcacc
    36961 ggttccgcct ggacatacgg cgcgcaaatg tcgaccatgc tgaacaggcg cggcgaaggc
    37021 accagcgcca gcgcatgaag cacatcatta gcattacaca cgatgttcga ttcatacggc
    37081 gttaacggtt cgaggattag atccatgccg atgttttccg cgtactcata cagttcgctt
    37141 aggttctctg ccagccgtcc ccagataaca ttaggtggcg tgagatagcc cgcgtgggcc
    37201 gcggaaatca gcgtataacc cgcgttcatc tcttttgcca tatccatcgc cagcttgatc
    37261 atgtcgaggc tttcgcgacg catatgttca tcgcccagca tcatgttata cggatagcca
    37321 ttggtttctg gcgtatagcc gataatcggc atctgatacg tctgcgccag cgccttgatt
    37381 tgtttgatgc cgcccgcttt taagtccggc gcgaacgcgt gcgggcgacc gccccaaatt
    37441 tcgatgccgt cgtaacccag ctcgcttgca tcacgaaatg cgtgttcaat aggcagccgc
    37501 tggtggccgc aggtaaacat acctgttttc atcgggtaac tccttgcgta aatccggccc
    37561 gttgccgggc cgggcgaggg attaatattc caccagacca ccgtaatagc ggcgttcatc
    37621 cgggttgtga tctttgtaaa tagacaggta gtagcagagc cactccattg gcacgaacat
    37681 caggaacggt gccagccacg ggtgcagccc ttgcgaaatt tcggcgtaat cgatgacgat
    37741 cacgttgtca gtacgctgtt taacaaagtt aatggcgcgt tcggtggtgt ggcgactttc
    37801 atcattgccg agcaggaaca ggaacggaac gcccggttcg acaatctcca gcgggccatg
    37861 gcggaactct ccgctctcaa tcacgcagcc gtgcgtccag gtaaattcca tcagcgttac
    37921 aatgccttct ttgtaaccca gcggacgcag cggacccgca gcaacggtat aaatcatcgg
    37981 ccactggctg gccagttcac caagctggcg gcctttttct tcccaggtgc gtaccagatg
    38041 accgagcgca ttcggcaact gcttgagatc gtttttgatc ttgccgattt ccgcgttcgg
    38101 cgcgaggcgg gtgatcatct ccagcaccac gctgtagcag agcagcaggt gaatttccca
    38161 gatacagtcg gcctgataat caatgctaaa ttccgccgcc gaggtaatcg ggctgtccgc
    38221 gcgtttggtg aacgccgcag tgagtgcgcc gcaggcccgg cccagctcca gcgctttgat
    38281 tacctcttcc gttttaccgt agtcagaaac gccaatcacc gcgcaacgat cgtcgaggcg
    38341 atacggggtg ttatcgcaga actcccagcc ggaaatggcg tagacctgaa gatcggaaaa
    38401 gcgatccgcc agatgtttcg ccgcttgcgc cgcgttgagt ggcgagccgc aggcaacgaa
    38461 ataaatacga tcaatgtcgc gcttcaccat ctcttccacg atggcgtgca ccagcggaac
    38521 gtcatggctg agaacttttt ccacttcctg aaccatattt tcggtgacca gaaagtccac
    38581 ggtgctttta tcaatatcca acattttatt ttctccagat aacattacga caaagcgttg
    38641 agctggcggc tacgcttcgc ccagaaggcg taagcaggca gaccagtagc aataacgatg
    38701 acggcgcaga taaggccggg aatcggtgcc cagacaaagg ttgaggcgac cagaatgagg
    38761 cttgacgcaa tggcgagggt ggtcatcagc ccgaaagcag gagtacgcca cagcggtttg
    38821 taatcgtcgc gtttacgaca ccagatgatg gagccgaagg tgagggtatt tttgaaacac
    38881 atcaccaggg tgaaataacc cagcaggctg gtgagatcgg aaacgaagat gaagaagagc
    38941 cccagcgccc cttgcaggat gatggagaca tccggcgtgt tgtatttcgg atgcacatgg
    39001 ccgaagcatt taaagaacag gttgtctttc gccatcgcgt attccagccg cggctggtac
    39061 atcacgcagc tggaaagcga accaagaatg acgatcatcg ccgtgatggc aacaaagatc
    39121 ccagcggtgc tgccgagtgc ggggatccag gtcagggcgt cggaaatcgg cgtttcagaa
    39181 ttggcgagtt tgtcgaaggg catcaggtcg gaaatcacca gcgccagcag ggtgtagagc
    39241 accagaacca gcagacagga accaatcagc gctcgtggca tggtttttcc ggggttttta
    39301 atttcgccgg tcatataaca gatagaggcc atgccggtat acgaccaact ggtggcagag
    39361 atccccgcca gcagcgccat aaagctgccc gttgcgccaa tcgcagtggt ggtaggggcg
    39421 gcaaaattct ccgctttaaa ccagaagatc ccaaggccaa tgacgatagt gaacgggata
    39481 attttggcga tggtaattag cgtctgaaac gctgcgccgc cttcaacgga gcgcaggtgt
    39541 agcaacataa aggcgataat taatccggcg gcgataaatt taccgggcaa cggatcgata
    39601 ggcgttaaaa agccaagatt gctgacaatc gccagcgcca taatcgacaa tgacggcgca
    39661 tcgttggccc agaagctggc ccagccggag aggaaagcca gcggtcggct tccggcattt
    39721 ttcagataaa cataatctgc gccattttcc ggataagcgg tggatagttc cgcatagacg
    39781 cacatttgcg ggatcacaat taacccgcca atgacaaacg tgagcaccgt aagccacggc
    39841 gtgcccgctg cttttgccac ttcacccaca gatacaaaaa taccggagcc gacggttgtc
    39901 ccgacggcga ttgcaagaac ggcccaaaat ccgagcttgc gttggagttc ctggcttccc
    39961 atagcgatac ctttttcttg tccaacacgt tgtaggggag agtttgcagt catttaaaag
    40021 gtaacatgac aatgcatgat gaatataata acatatgatg ttatgtaatg cggaggaaga
    40081 tcacaaaatt tcgcacagga tcgcgctgtg gctaatgaat gtagttatcc aattgaaatt
    40141 aaagtgaaaa tatttttacg ggtggggagc aagaaggata tagaaacaaa tacgccctcg
    40201 gaaaatccag agggcgtcgg gcaattaaac cggtgttagc cgatttctgt cagagactta
    40261 ctgtgcagta ggaccgcaac cgccaatgat tttagaaata gaaattgccg ggtgcagcag
    40321 gtagtcgtaa gagcagttct tatcacggtt ttcaatatga ccagtacatg cagttaacgt
    40381 agccagtact gcacctacca gtgcaacttt aataaatttg ttcatt
//
LOCUS       DAZPEB010000012        40275 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZPEB010000012 DAZPEB010000000
VERSION     DAZPEB010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40275
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.012"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(457..2148)
                     /gene="fruA"
                     /locus_tag="ACHURJ_000599"
     CDS             complement(457..2148)
                     /gene="fruA"
                     /locus_tag="ACHURJ_000599"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFR1279671.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAVARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(2165..3103)
                     /gene="fruK"
                     /locus_tag="ACHURJ_000600"
     CDS             complement(2165..3103)
                     /gene="fruK"
                     /locus_tag="ACHURJ_000600"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFR1279672.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(3103..4233)
                     /gene="fruB"
                     /locus_tag="ACHURJ_000601"
     CDS             complement(3103..4233)
                     /gene="fruB"
                     /locus_tag="ACHURJ_000601"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFR1279673.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFAVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            4601..5782
                     /gene="setB"
                     /locus_tag="ACHURJ_000602"
     CDS             4601..5782
                     /gene="setB"
                     /locus_tag="ACHURJ_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFR1279674.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALTREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRTKD
                     V"
     gene            complement(5779..6033)
                     /locus_tag="ACHURJ_000603"
     CDS             complement(5779..6033)
                     /locus_tag="ACHURJ_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFR1279675.1"
                     /translation="MECRPGCGACCTAPSIFSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAS"
     gene            6188..6760
                     /gene="yeiP"
                     /locus_tag="ACHURJ_000604"
     CDS             6188..6760
                     /gene="yeiP"
                     /locus_tag="ACHURJ_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFR1279676.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            6983..8449
                     /locus_tag="ACHURJ_000605"
     CDS             6983..8449
                     /locus_tag="ACHURJ_000605"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFR1279677.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLNE
                     QAPTLQIKDVDLTQYADKLIARFANPALKHKTWQIAMNGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            8567..9553
                     /gene="yeiR"
                     /locus_tag="ACHURJ_000606"
     CDS             8567..9553
                     /gene="yeiR"
                     /locus_tag="ACHURJ_000606"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFR1279678.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWTVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            9592..10305
                     /gene="lpxT"
                     /locus_tag="ACHURJ_000607"
     CDS             9592..10305
                     /gene="lpxT"
                     /locus_tag="ACHURJ_000607"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFR1279679.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGLWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTNINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            10717..11283
                     /gene="mepS"
                     /locus_tag="ACHURJ_000608"
     CDS             10717..11283
                     /gene="mepS"
                     /locus_tag="ACHURJ_000608"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFR1279680.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            11464..13020
                     /locus_tag="ACHURJ_000609"
     CDS             11464..13020
                     /locus_tag="ACHURJ_000609"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFR1279681.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGGETPEQARWLSEPGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            13102..14916
                     /locus_tag="ACHURJ_000610"
     CDS             13102..14916
                     /locus_tag="ACHURJ_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFR1279682.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADTLLNEAGWVLKGQQRVNATTGQQLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSHPAVRPVYSLGIDTWWYDVNKAAKLPSAKQQGE"
     gene            14917..16011
                     /locus_tag="ACHURJ_000611"
     CDS             14917..16011
                     /locus_tag="ACHURJ_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFR1279683.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     HNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTILTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            16011..17036
                     /locus_tag="ACHURJ_000612"
     CDS             16011..17036
                     /locus_tag="ACHURJ_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1279684.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            17038..18627
                     /gene="yejF"
                     /locus_tag="ACHURJ_000613"
     CDS             17038..18627
                     /gene="yejF"
                     /locus_tag="ACHURJ_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFR1279685.1"
                     /translation="MTQTLLAIEDLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFRGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNYAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKLQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(18631..18975)
                     /locus_tag="ACHURJ_000614"
     CDS             complement(18631..18975)
                     /locus_tag="ACHURJ_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFR1279686.1"
                     /translation="MTSLQLSIVHRLPQNFRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(19308..20491)
                     /gene="bcr"
                     /locus_tag="ACHURJ_000615"
                     /pseudo
     CDS             complement(19308..20491)
                     /gene="bcr"
                     /locus_tag="ACHURJ_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
     gene            complement(20519..21214)
                     /gene="rsuA"
                     /locus_tag="ACHURJ_000616"
     CDS             complement(20519..21214)
                     /gene="rsuA"
                     /locus_tag="ACHURJ_000616"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFR1279687.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            21363..23123
                     /locus_tag="ACHURJ_000617"
     CDS             21363..23123
                     /locus_tag="ACHURJ_000617"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFR1279688.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            23248..23532
                     /gene="rplY"
                     /locus_tag="ACHURJ_000618"
     CDS             23248..23532
                     /gene="rplY"
                     /locus_tag="ACHURJ_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFR1279689.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLAIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(23671..24678)
                     /gene="yejK"
                     /locus_tag="ACHURJ_000619"
     CDS             complement(23671..24678)
                     /gene="yejK"
                     /locus_tag="ACHURJ_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFR1279690.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTKFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            24860..25087
                     /locus_tag="ACHURJ_000620"
     CDS             24860..25087
                     /locus_tag="ACHURJ_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFR1279691.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            25107..26867
                     /gene="yejM"
                     /locus_tag="ACHURJ_000621"
     CDS             25107..26867
                     /gene="yejM"
                     /locus_tag="ACHURJ_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFR1279692.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRPQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALLDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            26942..27018
                     /locus_tag="ACHURJ_000622"
     tRNA            26942..27018
                     /locus_tag="ACHURJ_000622"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26976..26978,aa:Pro,seq:ggg)
     gene            27237..28478
                     /locus_tag="ACHURJ_000623"
     CDS             27237..28478
                     /locus_tag="ACHURJ_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HFR1279693.1"
                     /translation="MARKTPPLTTVQIKAARPAEKEYTLQDGGGLFLLVKPSGSKLWR
                     FSYYRPSDKKRILLSFGSLDDVSLADARKRRSEYRALISAGTDPQGHEKKKREAEARR
                     QGNTFENVAAAWYQVKISQNLAPNTIKDIWRSLDKYVFPFIGNTPIDTLTARRFVEVL
                     TPIKERGNLETLKRVLQRVNEVMDYAANSGLIDANPAMNVRKAFPSPVKKHMPTIRPE
                     QLPELMQALSVSATERQTRLLIEWQLLTVTRPAEASSTRWDEINLDAKQWTIPAGRMK
                     MRRDHVIPLSGQAMAVLEAMKPISHHRNYVFPSLKDPQQPMNSQTANAALRRMGFAGV
                     LVSHGLRAIFSTAANEEGFEPDVIEAALAHVDTNEVRRAYNRSNYIEKRIVLMRWWGE
                     FVEAAATGVTLASGKRGIRAV"
     gene            28577..28807
                     /locus_tag="ACHURJ_000624"
     CDS             28577..28807
                     /locus_tag="ACHURJ_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001171356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1279694.1"
                     /translation="MQKNIRCNCDGLQLALMVQHEFWSTYDPEDRTTAPSKKQVVDFL
                     VSRGASRNLAVSIDKVVRPASMKIGGRPKKWR"
     gene            28974..29180
                     /locus_tag="ACHURJ_000625"
     CDS             28974..29180
                     /locus_tag="ACHURJ_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR1279695.1"
                     /translation="MEAIRKIIFRQEVKKIIHIKADSTLQSMINAGEFPQGFRVGLRR
                     RGWYEDDVLAWQKEREQEARGTAA"
     gene            29195..29860
                     /locus_tag="ACHURJ_000626"
     CDS             29195..29860
                     /locus_tag="ACHURJ_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1279696.1"
                     /translation="MTNTKKAAPIWSRLSEQLTRCALCVYDPKHKHGDDSRYQAGGQC
                     NQSGSVRCHTCNERFSLYSLRNCSRAKAHGANLSDSCSIFLRRLFRAGDNVLVNSLSV
                     IVLSCIAMRRNTSHHGADSFYPLGSMPRRFSSVRPNSLSQLARLMPSLCASLSNCSFS
                     SGEIRILNCGDCPSPLGLLSRLIIDKWSPIELAFPLLGGHLNTRALKKAKPRKCHYHS
                     QGF"
     gene            29886..30086
                     /locus_tag="ACHURJ_000627"
     CDS             29886..30086
                     /locus_tag="ACHURJ_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFR1279697.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            30076..30297
                     /locus_tag="ACHURJ_000628"
     CDS             30076..30297
                     /locus_tag="ACHURJ_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1279698.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            30290..30523
                     /locus_tag="ACHURJ_000629"
     CDS             30290..30523
                     /locus_tag="ACHURJ_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1279699.1"
                     /translation="MRDDRFNSLKQEFPGVPDDAADALSSMPELIRAAFFLLSTREYK
                     STGLDVLNIAADYAEYVAEARYRRKFPEDVSHA"
     gene            30516..30749
                     /locus_tag="ACHURJ_000630"
     CDS             30516..30749
                     /locus_tag="ACHURJ_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1279700.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            30755..31054
                     /locus_tag="ACHURJ_000631"
     CDS             30755..31054
                     /locus_tag="ACHURJ_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFR1279701.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGTVMIKGVEERRVTY
                     RREGYEYDCVMPVYQFRRDFSLVQTAPHNVPTSNARARANIQKLKTMINGFRGKK"
     gene            31051..32451
                     /locus_tag="ACHURJ_000632"
     CDS             31051..32451
                     /locus_tag="ACHURJ_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="HFR1279702.1"
                     /translation="MKSAPNLKKQPYDKMTEVIIFAGSDAWAHAKQWQEQDGRLAGDN
                     VPPVVLADDQLDELADLRIIDEGRYCVRLYKAGHIRPSNINAIAHKLAAAGVTDANYY
                     PEGMHSHMRENWREYLERVRGKEPVEEKNHQRKTTLPMSVGSTGYDTQLDYVVKGIIP
                     AVSLCSIYGASGSYKSFLAGSWACHVATGRQWGGRRVAHGAVLYVVGEGGIGVPRRVK
                     AWEVVHDEQVKNLYLVNRPIFPAAPLDVDEMVIAARQVERETGKPVRMIILDTLARCF
                     GGNDENDSRDMGAFIRGCDELKRRTGATVLVVHHSGKDETKGARGSSAFRASLDAEYR
                     IRREDAGSKALVISCTKMKDAEELKEAAYDLRVVELFTDADGELITSLVVVDDPRPPV
                     ELERIEEAGNKTENHTALWGCIRSRTQNGDKCTIPLLRDDMKKLGYEMKNFRRWLYKL
                     EKDGVISIDGDDVAPL"
     gene            32652..32903
                     /locus_tag="ACHURJ_000633"
     CDS             32652..32903
                     /locus_tag="ACHURJ_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1279703.1"
                     /translation="MTQKRRDRTEPKYKALDMTEHTLKVAIRTIDRHTREGYAKEHPD
                     LISAFMTTAAANFATLTEREIAEAEQVTTINVKTGEVEL"
     gene            32900..33310
                     /locus_tag="ACHURJ_000634"
     CDS             32900..33310
                     /locus_tag="ACHURJ_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFR1279704.1"
                     /translation="MTAQIAAYGRLVDDPQVKQTSKGTPMTLARMAVSLPCSQAQDGQ
                     ATLWLSVIAFGKQADFLAKHQKGDVASVSGTMQVSQWTGQNGETRQGWQVIADSVISA
                     RAARPGGNRRKTTGTQGNQPPAGGDDPYGDDISF"
     gene            33321..33593
                     /locus_tag="ACHURJ_000635"
     CDS             33321..33593
                     /locus_tag="ACHURJ_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFR1279705.1"
                     /translation="MVHDRIAEELEAKGFYRRAAARWGEVMQLVETDKERHHITMRRL
                     ECSRKAQRAPEPPDNFGDLKKAVDRTYAEMGIDGAGDEIWRNYQDS"
     gene            33550..33677
                     /locus_tag="ACHURJ_000636"
                     /pseudo
     CDS             33550..33677
                     /locus_tag="ACHURJ_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001301569.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
     gene            33719..33943
                     /locus_tag="ACHURJ_000637"
     CDS             33719..33943
                     /locus_tag="ACHURJ_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1279706.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMSINDYRAFLETGALIEKDQHG
                     FVRCALSGEMLAVNPEQIDALIEFLKEIRD"
     gene            34195..34401
                     /locus_tag="ACHURJ_000638"
     CDS             34195..34401
                     /locus_tag="ACHURJ_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1279707.1"
                     /translation="MAEEKKGGVSVYISPEIVEVLKQRHKKNYEAGVAAGLDPLMTPE
                     PSIGSLVRSYLLAALGMHKNYGGE"
     gene            34401..35456
                     /locus_tag="ACHURJ_000639"
     CDS             34401..35456
                     /locus_tag="ACHURJ_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFR1279708.1"
                     /translation="MAGKATALNTNQLFMYLNRGDIADFKFSPLFTTLFFPNVATFST
                     QNIMLDTLDIEEVTMSAFCSPMVGSQVQRDKGYETSTIKPGYMKPKHEIDPTKTIMRM
                     AGEDPAQLNDPTYRRMRLITGNMRRQINAIKARVEWLAVNAVTTGKNIIEGEGIERYE
                     IDWKIPEKNIIEQSDGKKWSEHDKDIYDPIYDIELYADQAGCPANVMIMGAEVWRTLR
                     SFKKFRELYDLSRGSESAAEMACKNLGEVVSFKGYLGDIALIVYSGKYTDSDGTEKYF
                     LEPDLLVLGNTNNKGLVAYGAIMDQEAVRTGATQNMYYPKNWIEDGDPAIEYVQTHSA
                     PQPVPADIRKFVTVKIG"
     gene            35468..35803
                     /locus_tag="ACHURJ_000640"
     CDS             35468..35803
                     /locus_tag="ACHURJ_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFR1279709.1"
                     /translation="MDTPYIELFAGSQQVSTTLVHFAADAGVIQEFTPLMLVDNGEFK
                     PWDGQESGKAVYLTSYLVDTSKQKSAQCYKTGIFNIAAVNWPESVDTDAKKCAAFAGS
                     GVSVQPLAR"
     gene            35816..36229
                     /locus_tag="ACHURJ_000641"
     CDS             35816..36229
                     /locus_tag="ACHURJ_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HFR1279710.1"
                     /translation="MATNESIMALPLASKFKAEARAMADRGLSTYEAVYQLNKLEEQD
                     KPRADAIMALHEHKDYQPLLRAMANVPCIDVDTAKNILNMTIEQERPKVAPELTAAFE
                     NFMDMHSPQTVSAGMAYDGRNPGDDGDIDRILKTI"
     gene            36391..36825
                     /locus_tag="ACHURJ_000642"
     CDS             36391..36825
                     /locus_tag="ACHURJ_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1279711.1"
                     /translation="MIDKACFVSQQEIAEHFKVNRTTIRAWTKQGMPYLNADRGKSGG
                     YHIGHTLLWSSGKSRLEAIRYHVETSALEKIMFARLLSSERDEYSSEETEHRFDEGLQ
                     IYGYSPEDVSKARNKMAGFLAGWRHAVSVRRASMEQSADTEQ"
     gene            37106..37387
                     /locus_tag="ACHURJ_000643"
     CDS             37106..37387
                     /locus_tag="ACHURJ_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1279712.1"
                     /translation="MTEAEILGLIRRAGGISQQTDEQATQPDSVTAENYARVVAEVMR
                     RDGIQLNDVDMRNIRIRVLEMLAYNRRVALYRETEKITYHWKKPERLRR"
     gene            complement(38065..>40275)
                     /gene="yejO"
                     /locus_tag="ACHURJ_000644"
     CDS             complement(38065..>40275)
                     /gene="yejO"
                     /locus_tag="ACHURJ_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HFR1279713.1"
                     /translation="PTIKGGRLIVEKDGITVLAAIEKGGLLEVKEGGLAIAVDQKAGG
                     AIKASTRVMEAFGINRLGQFEIKNGIANNMLLENGGSLRVEENDFAYNTTVDSGGLLE
                     VMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYELDDGSGLIVME
                     DTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSSKAISENMVINN
                     GRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGASFRTHGAVDTSK
                     ADVSLENSVWAIIADITTTNQNTLLNLANLAMSDANVIMMDEPVTRSSVTASAENFIT
                     LTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASPAAGDSLTLVTT
                     GGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTTDVLNMAAAQPL
                     VFDAELDTVRERLGSVKSVSYDTAMWSSAINTRNNVTTDAGAGFEQTLTGLTLGIDSR
                     FSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNGAYVDGVVKVDR
                     FANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAFTGFTTDGQDYT
                     LSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEYADSNHVKVNDD
                     GKFNNDVAGTPGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQAGVVWTF"
BASE COUNT        10166 a        10224 c        10079 g         9806 t
ORIGIN      
        1 agcctataat tacgacataa tccttttcgc gcatagcaca tcctctggta aaaaattacc
       61 aaccctgtca atccacaaga atatagatac tgcaaagacg gtttaaacat gtgttcatat
      121 ttaaacatgt gctcatagca tgcatgtttt acgtaaagta aattaccgcc agagaggcaa
      181 aatgggcaga tttgagcagg ctcacaaaat gcgattaaat gcctgaatta caaaagcgag
      241 gaatttgtga cgggatgcac agaacgggaa caataaaaaa ggcagaaaat aatctgccct
      301 tgaggaaata accttagtgt ttgatgaacg tttcaagttt ctactgaaag ataaaaccat
      361 ttaaattcat aaaattacat tcacaaaccg gtcattctct ttgcgctcat caaatgttac
      421 agggcaggaa atttctgccc tgtaacacac cttttattac gctgctttcg ctactgcgtc
      481 cacttccgga cgtttcagga aggcataggc caaacccgcc accagcgtac cggcaataat
      541 tgctaccagg taacccagta ccggcgtaat agcgccaggg atcagcagaa caaacagacc
      601 accgtgcggt gccatcagtt tcgcaccaat cgccattgag attgcgccag tcagcgcccc
      661 acccacgata cagcacggca gcacacgcat cggatcacga gcagcaaacg gaattgcacc
      721 ttccgaaatg aagcacagtc ccaataccag agcggctttg ccaccttcct gctgcgcttt
      781 gtcgaatttg cgacgcgcca ccattgttgc cagacccatt gccagcggtg gcaccatacc
      841 tgccgccata atcgccgcca tcgggccata ggtttgagta ctcagcagac ccacaccgaa
      901 tgcgtacgct gctttgttta ccggaccgcc catgtcagta cacatcatgc caccgaggat
      961 cgcccccagc agaaccgcat tcgcagtccc catggtctgc agccagtgag tcagcccttc
     1021 gagaatgcca gcaactggtt taccgatcag gtagatcatc gccagaccga ccaccagact
     1081 ggaaattagc ggaatgatca ggatcggttt cagcgcctcc atactctgtg gcagtttcag
     1141 ttgcgtactg attaacttcg caatgtaacc agccaggaag ccagcaatta taccgccaat
     1201 aaagccagaa ccggtgctga ccgccagcat accgccaatc agacccggcg tgaggcccgg
     1261 acgatcggca atggagaagg caatataacc tgccagtacc ggcaccatca gcgcaaaggc
     1321 tgaaccaccg ccaatctgca tcagcgccgc tgccaacgta cccggctctt taaacgcttc
     1381 gataccaaaa gcaaaagaaa gcgcgataca cagaccacct gcgacgacca tcggcagcat
     1441 ataagagacg cctgtcagca agtgacggta agcgcctgca ctctctttct taccttcagt
     1501 ggtcgccgtt tgagctttgc ccgccggttc atacggcgtt gcttcagcaa ccgctttatc
     1561 cagttcctgc gcggttttct tcagcgccag accggtagag gtacgataca tcggtttacc
     1621 agcaaatttc gccagatcca cttcgatatc tgccgccaca atcaccagat ccgctgctgc
     1681 gacttcttca ggggtgattg cattgcccgc gccaacagaa ccacgggttt caactttcac
     1741 ccaccagcca cgttttttcg cttcggtttc aatggcttca gccgccataa aggtgtgtgc
     1801 tacgccagtc gggcaagcag tcaccgcaac tacgcgtttc ggaccgctgg cagcaaccgg
     1861 tgctgtcgca gcgaccggcg cagtgtaagg tttcgcatgg cctttggctt cactcaggaa
     1921 cagctcaggg tgcgcaactg cccgggaaat atcacccagc cagacatttt taccgttcag
     1981 cgcgctgtca ttcgggatgg aatcaccgag aacaatcgcc atttctgcgt cgttcggatt
     2041 gtcgatgatt tccagttttg cttttcgcgc caccgcgccc agcagggtct tcgccatata
     2101 ggcgcgtgcc tgaccgagat tagcgtcaat aatcagcagc gttttcatta tgcctctcct
     2161 gctgtcagtt aaaaggttgt aagtcgacgc gcgccatcat tgcggccaac tgcggacgat
     2221 cggtaatacc cacattgctt tgacttaccg ccagggctgc aacagctgtc gccagacgca
     2281 gtgtgtgttc actggattca cgcatcagca agccataaat caggccacca accatagaat
     2341 cccctgcgcc aacggtgctt acgacatcga ctgacggtgg tttggcgatc cattcgccgg
     2401 aggcattaac ccaaagcgcg ccttcggcac ccagtgaaat aacaacatgc gcaatacctt
     2461 gttcacgtag cgcatgtgca gcttcaatca catctttcat ttcaggcagt ttacggcctg
     2521 cccaaatttc caactcgcgg cggttaggtt tcaccagcca cggtgccgct ttcaaacctg
     2581 ctactaacgc ttcacggcta ctatcaaaga taatgcaagg acactgacta cgcaggcgag
     2641 tcatccagtc ggtgaacgct tccgggctga cgcctgacgg taagcttccg ctgacacaga
     2701 ccatatcgaa ctgaccgagc cagctcagag aatcagtcac aaagcgttcc cagtcggcgg
     2761 gggtgacttc aaaacccgag aagttgaagt cggtcacttc gccgtctttt tccgtcagct
     2821 taacgttaat tcgggtgcgc ccctgtacaa cctggaaacg gttggcaatg cccagctcgc
     2881 tgaacagttg ctgaaaacca tcctgattgt ctttacccag gaagccgcca acggtgacat
     2941 caattcccag gtcttttaat actttggcca cgttgatgcc tttacccgcc gcatgcagac
     3001 cggtggtttt caccaggttc acttcgccgc gttcaatttc cgggcagaaa ccaacaaggt
     3061 cataagccgg attaagggtg atcgtagcaa cacgtctgct cattatgcgc cctccccaag
     3121 accagcagcg atagcgtcgc caatggcttt cagcgcctgt tcagcatctg caccctgggc
     3181 ggtaaagcgt aggcgatgac ctttcttaac gccaagtgcc acaactttca tcagactacg
     3241 tccgtttgcc ggtttgccgg tgccatcaag gtttgtcacg gtaatatcac tgttaaattg
     3301 tttaatggta ttgaccagca tggtacctgg acgagcatgc aggccgtgtt cattgcgcac
     3361 cacaaactcc gcgcttaaca cgtcgtcggt cggcgcatca tcgctggtca gcagcgccag
     3421 caacgttgcc gcatctgctt tcagcaagcg gtcagcttta ttgtcgagca ataaatcagc
     3481 gagacgctta agaaccgcga tgggctgatc gtcattcatc gccacactca ccagcatggc
     3541 tgccgtttcg ccgtccacag caaaagcatt tgccgcacgg cttaccgcaa tcgcgctacg
     3601 cagattgcct tcggcgctat cgctcagcca gataccctgt ccgagattca gcggttgttc
     3661 attgatggct ttggtgacga aagtggcgtc aactgccccc gcctctttca gacgcgcagc
     3721 gttcagcgcc tgaagagtca gcagatcgct ggcgacgata tccagcgtca gcatttcgtt
     3781 gtcgagcttc agctgctcac tctgcttttc gcccatcagt aatgcgcgaa gttcttctgc
     3841 tgttgttgct gacttcagtt gttcagcaac ggaatcatcg ctcagtacgt gggtcagctg
     3901 gcgtagcagg cccagatgtt catccgagct ggcagcaata ccgattgcca cgtacgctac
     3961 ctgaccgtca ccccaggtga cgccttccgg gaactgaaat acctgaacgc cggttttcag
     4021 cacctgatcg cgggtgtcgg tagtgccgtg tggaatagca ataccattgc cgaggaacgt
     4081 tgaggtttgc tgttcgcgcg ccagcatgcc attgacgtag ccttctgcta cattaccggc
     4141 ctgcaccagc gccgcagcga cctggcgaat cgcctcttct ttgtctccgg ccttttcgcc
     4201 cggatggatg tcctgtacgg ataactggaa catagttctc ctctcttgct gaattgaaac
     4261 gattcagcct ctatgagaaa aaaagcgcca acctggctta gggttaaaga caagatcgcg
     4321 ctgaaacgtt tcaagaaagc ataatacttc tgtttcagca cgcaaggaaa gccgtaaagt
     4381 tatgagcaaa agtttgatgt gctgcacatt tttttgtcat ttttcctaat tgctgatggg
     4441 aaaatccgtt atcagcaatt tcatttcagc agcgtcagca gccaatcagc aacagtgaaa
     4501 tggcattttg attattcgtg gcgcttttga tttcgctgtc tgtttatttt ctgacaagca
     4561 gcgtaaactc cgcgtcttcc tcttccagtg atagacctgc atgcataact cccccgcagt
     4621 ctccagcgcg aaatcgtttg acctgacctc gacggcgttt ttaatcgttg cctttctcac
     4681 cggtattgcg ggtgctctgc aaaccccgac actcagtatt ttccttaccg atgaagtaca
     4741 tgcccgtccg gcgatggtgg gattcttctt taccggcagc gctgtcattg ggattctggt
     4801 aagtcagttt ctcgctgggc gctctgataa gcgcggcgat cgcaaatcgc tgattgtctt
     4861 ttgctgcctg ttaggcgtgc tggcctgcac cctttttgcc tggaatcgca actactttgt
     4921 tttgctattc gttggcgtct ttcttagcag ctttggctcg accgctaacc cgcaaatgtt
     4981 tgcccttacc cgtgaacatg ccgacaaaac cggacgtgag gcggtgatgt tcagctcttt
     5041 tttacgcgct caggtttcac tggcatgggt cattggccca ccgctggctt atgcgttagc
     5101 gatgggtttc agctttacgg taatgtatct gagcgcagcg gtagcgttta ttgtttgcgg
     5161 cgtgatggtg tggctgtttt taccgtcgat gcaaaaagag cttccgctgg cgaccggcac
     5221 ggttgaagcg ccgcgccgta accgtcgcga tacgctactg ctgtttgtca tttgtacatt
     5281 gatgtggggc tcgaacagcc tgtacatcat caacatgccg ctatttatta tcaacgaact
     5341 gcatcttccc gagaaactgg ccggtgtgat gatggggacc gccgccgggc tggaaatccc
     5401 gacgatgttg attgccggat atttcgccaa acgtctgggt aagcgtttct taatgcgcgt
     5461 tgctgccgtg ggtggcgtct gtttttacgc aggaatgctg atggcgcatt cacctgccat
     5521 tctgttgggc ttgcagctgc taaatgctat ttttattggc attctgggcg gcatcgggat
     5581 gctctatttt caggatttga tgcccggtca ggcaggttca gccaccacgc tctataccaa
     5641 cacgtcgcgc gtgggctgga tcatcgcagg atcagtggcg ggcatcgtcg ccgagatctg
     5701 gaattatcac gctgtgttct ggtttgcgat ggtgatgatt atcgccactc tgttttgctt
     5761 actgcggact aaagatgttt aagacgcggt cagcatttcc agatcgataa gccaggtcat
     5821 ggcctgctga cgcgagttac cgcacatttc agcgctggct tgtaagcctg cacagacttt
     5881 cggtcgcagt ggcgaggtga aaattttaca gcgttgctgt tcatcaagct gaatgcaggg
     5941 cgtattggcg ggcttgccat ctggcatacc gggaataggg ctgaaaattg aaggggcagt
     6001 gcaacacgcc ccgcaacccg gacggcattc catacgttgt tctcttaagg ttaaccaccg
     6061 gattgatgag cgcgcacagt accacctttt ttgcaccagc aaaagtgcga ataccacttg
     6121 cccgaaaggc ccgtcgcgag tactttgtcg cgatattttt gacattttcg actacaggaa
     6181 tttttcgatg ccaagagcga acgaaattaa aaaaggtatg gtactgaatt acaacggcaa
     6241 actgctgctg gttaaggata ttgatattca gtcgcccact gcccgcggtg ccgctacgct
     6301 gtacaaaatg cgtttttctg atgtccgtac cgggctgaaa gtagaagagc gtttcaaagg
     6361 tgatgatatc gttgacaccg tgacgctgac ccgccgttac gttgacttct cctatgtcga
     6421 tggcaacgaa tatgtcttta tggataaaga agactatacc ccgtatacct tcaccaaaga
     6481 tcagattgaa gaagagttgc tgtttatgcc agagggcggc atgccggaca tgcaggtgct
     6541 gacctgggac ggtcaactgc tggcgcttga gctgccgcag accgttgatc tggaaatcgt
     6601 tgaaaccgca ccaggcatca aaggggcatc tgccagcgcc cgtaacaaac cggcgacatt
     6661 gagcactggt ctggtgattc aggtaccaga atacttaagc ccgggcgaaa aaattcgtat
     6721 ccatatcgaa gaacgccgtt atatggggcg tgctgactaa cttcagccgc atgcagaaaa
     6781 gggatagctc aggctgcccc ttttttaatt tattatacca atcttctatt ttgcgcttcc
     6841 tgaacacctt acgccctcaa ttttcactcg ttgattgatc gccctcacac ttcatcgcat
     6901 taacaatcca gaccaatttc aattgctgtc atataacttt acactgtcat tgttaattta
     6961 tcgttactaa gacgtgactc ctatgaagac aattgcctcc gttacgctcc cgcatcatgt
     7021 acacgctcca cgctacgatc gccagcagtt gcaatcacgt atcgttcatt ttggctttgg
     7081 cgcctttcac cgcgctcatc aggcgttact gaccgatcgt gtgctgaatg cccagggcgg
     7141 cgactggggg atctgtgaaa tcagcttgtt cagcggtgat caactgatga gccagctccg
     7201 cgcacagaac catttatata ccgtgctgga gaaaggtgcg gacggcaatc aggtgataat
     7261 tgtcggtgcc gttcacgaat gccttaatgc aaaactggat tccttagcgg caattattga
     7321 gaaattttgc gagccacagg tggcaattgt ttccctgacg attaccgaaa aaggctattg
     7381 tattgacccg gccaccggtg cactcgacac cagtaatccg cggattattc acgatctaca
     7441 aacccctgaa gaacctcact ccgcaccggg tattctcgtc gaagcactga aacgccgccg
     7501 tgagcgcggc cttacaccgt ttaccgtgct ctcctgcgac aatattcccg acaatggcca
     7561 tgtggtgaaa aacgcggtgc tgggaatggc agaaaaacgt tcgccagaac tcgccgggtg
     7621 gataaaagaa cacgtcagtt ttccgggaac catggtcgac cgcattgttc cggctgcaac
     7681 cgacgaatca ctggcagaaa tcagccagca tctgggggta aatgatccct gcgcgattag
     7741 ctgcgaaccg tttatccagt gggtggtgga agataacttc gtcgctgggc gtcctgcctg
     7801 ggaagtcgca ggtgtacaaa tggtgaatga tgtcctgcca tgggaagaga tgaaactgcg
     7861 tatgcttaat ggcagccact cttttctcgc ttatctgggt tacctttcag gattcgccca
     7921 tatcagtgat tgcatgcagg atcgcgcatt tcgccatgcc gccagaacat taatgctgaa
     7981 tgagcaagcg ccgacactac aaattaaaga tgtcgattta acacaatatg cggacaagtt
     8041 aattgctcgt tttgctaatc cggcgctgaa acataagacc tggcaaatcg caatgaatgg
     8101 tagccagaaa ttaccgcaac gtatgctggc aggtattcgc atacatctgg ggcgcgaaac
     8161 ggactggtcg ttgctggcat taggcgttgc tggctggatg cgttacgtca gcggcgttga
     8221 tgatgccgga aatgccattg atgttcgcga tccgcttagc gataaaattc gcgaacttgt
     8281 tgcgggcagc agcagtgaac aacgcgtaac cgccctgctt tccctgcgtg aagttttcgg
     8341 tgatgatctg ccagataacc cgcattttgt gcaggccatc gaacaagcct ggcaacaaat
     8401 cgtacaattc ggcgcacatc aggcgctatt aaacaccctc aaaatttaac gatttctgcg
     8461 gttaaagcgg atgaagctca ccttcgtccg ctctcccctt ctcttttctg ccttttttag
     8521 ccaggattaa cgctcagtta acttaccaga gtattgcgga gccgttgtga ccaggaccaa
     8581 cctcatcacc ggttttctcg gcagcgggaa aaccacgtcg attcttcatc tgttagccca
     8641 taaagatccc aacgaaaaat ggacggtact ggttaatgaa tttggggaag tcggaattga
     8701 tggtgctttg ctcgccgata gcggcgcatt gctgaaagag atccccggcg gctgcatgtg
     8761 ctgcgttaat ggtttaccca tgcaggtagg gttgaatacc ttactgcgtc agggaaaacc
     8821 agaccgcttg ttgatagagc cgaccgggct gggccatccg aaacagatcc tcgatctgtt
     8881 aaccgcacca gtctatgaac cgtggataga tctgcgcgcc accttgtgca ttctcgatcc
     8941 acgcttgctg ctggacgaaa aaagcgccag caatgaaaac ttccgtgacc agctggctgc
     9001 cgcagacatc attgtcgcca ataaatccga ccgtgcgacg cccgaaagtg agcaagcgct
     9061 acagcgttgg tggcagcaaa atggtggcga tcgacaatta attcacagtg agcatgggaa
     9121 agttgacggt catcttctgg atttgccgcg tcgcaattta gccgagttgc ccgccagcgc
     9181 cgcgcattct catcagcatg tcgtgaaaaa agggttagca gcgttaagcc tgccagagca
     9241 tcaacgctgg cgtcgcagtc tgaacagcgg gcaaggatat caggcctgcg gctggatatt
     9301 cgacgctgat acggtattcg acaccattgg cattctggaa tgggcgcgac ttgcaccggt
     9361 ggaacgcgtc aaaggcgtgc tgcgtattcc cgaagggctg gtacgaatca accgtcaggg
     9421 cgatgacctg cacattgaaa cgcaaaacgt tgcgccaccg gacagccgta ttgagctgat
     9481 ttccagcagc gaagctgact ggaatgcctt gcagagcgcg ctgttgaagc ttcgtttagc
     9541 gactaccgcg taaggttgcc tgcgtttttc agtaagataa ttagagaaaa tatgattaaa
     9601 aatttgccgc aaatagtgtt gttgaatatt gtcggcctcg cgctgtttct ttcctggtat
     9661 attcccgtta atcatggatt atggttgccg attgatgcgg atatttttta tttctttaat
     9721 cagaaactgg tcgaaagtaa ggccttttta tggctggttg cattgaccaa caatcgcgct
     9781 ttcgacggtt gttctctgct ggcgatggga atgttgatgc tgagtttctg gctgaaagaa
     9841 aacgccccag gcagacgacg tatcgtgatt attggtctgg taatgctatt aactgcagtg
     9901 gtattaaacc agctgggtca ggcattaatt cctgtaaaac gggccagccc aacattgacg
     9961 tttactaata ttaaccgcgt cagcgaactg ctctctgtcc ccacgaaaga tgcctcacga
    10021 gatagctttc ccggcgatca cggcatgatg ctgcttattt tttcggcatt catgtggcgt
    10081 tatttcggta aagttgcagg tcttatcgcc cttattattt ttgtggtttt tgcgtttccc
    10141 agagtaatga ttggcgcaca ctggtttact gacatcattg tcggttcgat gaccgtgata
    10201 ttgatcggtt tgccctgggt gttgctgacg ccattaagtg atcgattaat cacctttttt
    10261 gacaaatcac taccgggaaa aaacaaacat ttccaaaaca aataactcac agtaattaac
    10321 atcatcaggt ttatttttat agtgaggata atcctgatga tgcgcaccgt gctttcatct
    10381 atcgaacgca aaaatcattc tctaagtaaa tgaatggatt gcatgcgttt cactcaatcg
    10441 tactttaatt gaccaatccc gcttattaac tttctgtatc actttttctt ataaaaaatc
    10501 atgtaaaacc gctcgccaag accgcaccaa tcgggtaatc tcgaactcgt tttgcctcgg
    10561 cggtagatta tcctcacagc atataatttt gtgcgttagt ccacagattt ggccttaagg
    10621 aattgtttca acatgcccag gtaattagtc tcgtgtcgct tggcattttt ttataacgat
    10681 atttgtcgtt aaggacttca agggaaaaca aacaacatgg tcaaatctca accgattttg
    10741 agatatatct tgcgcgggat tcccgcgatt gcagtagcgg ttctgctttc tgcatgtagt
    10801 gcaaataaca ccgcaaagaa tatgcatcct gagacacgtg cagtgggtag tgaaacatca
    10861 tcactgcaag cttctcagga tgaatttgaa aacctggttc gtaatgtcga cgtaaaatcg
    10921 cgaattatgg atcagtatgc tgactggaaa ggcgtacgtt atcgtctggg cggcagcact
    10981 aaaaaaggta tcgattgttc tggtttcgta cagcgtacat tccgtgagca atttggctta
    11041 gaacttccgc gttcgactta cgaacagcag gaaatgggta aatctgtttc ccgcagtaat
    11101 ttgcgtacgg gtgatttagt tctgttccgt gccggttcaa cgggacgcca tgtcggtatt
    11161 tatatcggca acaatcagtt tgtccatgct tccaccagca gtggtgttat tatttccagc
    11221 atgaatgaac cgtactggaa gaagcgttac aacgaagcac gccgagttct cagccgcagc
    11281 taataaaccg tttggatgca atcccttggc tatcctgacg agttaactga aagcactgct
    11341 taggtagtgc ttttttgttt tcattcatca gagaaaatga tgtttccgcg tcttgatcca
    11401 ggctatagtc cggtcattgt tatcttttaa atgttgtcgt aatttcagga aattaacgga
    11461 atcatgttca tacgcgctcc caattctgga cgtaagctcc tgcttacctg cattgtagca
    11521 ggcgtgatga ttgcgatact ggttagctgc cttcagtttt tagtggcctg gcataagcac
    11581 gaagtcaaat acgacacact gattatcgac gtacaaaagt atctcgatac ctattttgcc
    11641 gacctgaaat ccactactga ccggcttcag ccgctgacct tagatacctg ccagcaggct
    11701 aaccccgaac tgaccgctcg cgcggcgttt agcatgaatg tccgtacgtt tgtgctggtg
    11761 aaagataaaa aaacattctg ttcatctgcg actggtgaga tggacattcc actcaatgaa
    11821 ttgattccgg cgctcgacat taataaaaac gtcgatatgg cgatcttacc cggcacgccg
    11881 atggtgccga acaaacccgc aatcgtcatc tggtatcgca accctttgct gaaaaatagc
    11941 ggtgtgtttg ccgctctgaa tctcaacctg acgccttctc tcttttatag ttcacggcag
    12001 gaagattacg atggcgtcgc cctcattatt ggcaatactg cgctatctac cttttcttca
    12061 cgtttgatga acgttaatga attaaccgac atgcctgtcc gtgaaactaa aattgcgggc
    12121 attcctctga ccgttcggct ttatgcggat gactggacat ggaacgatgt gtggtacgca
    12181 tttttactgg gcggcatgag tggaactgtc gttggcctgc tctgctatta cctgatgagc
    12241 gtacgtatgc gccccggcag agaaatcatg accgccatca agcgcgaaca attttacgtg
    12301 gcgtatcaac cggtggtgga tacacaagct ttgcgagtaa cgggcctgga agtactgcta
    12361 cgctggcggc atcctgtcgc gggagaaatt cccccggatg ccttcattaa ctttgccgaa
    12421 tcgcaaaaga tgattgtgcc gctgactcag cacctgtttg agttaattgc ccgcgatgcc
    12481 gcagaattag aaaaagtgct gccagtaggc gtcaaatttg gtattaacat tgcgccggac
    12541 catctgcaca gcgaaagctt taaagcagat atccagaaac tgctcacttc cctgcccgca
    12601 caccatttcc agattgtgct ggaaattacc gagcgcgata tgctgaaaga gcaagaagcc
    12661 acacaactct tcgcctggct gcactcggtc ggcgtagaaa ttgctattga tgacttcggc
    12721 accgggcaca gcgcgcttat ctatcttgag cgttttacgc tcgattatct gaaaattgac
    12781 cgtggattta tcaacgccat cggtacggaa acgatcactt cacccgtact tgacgcggtg
    12841 ctgacgctgg cgaaacgcct caatatgctg acggttgctg aagggggcga aacgccggaa
    12901 caggcgcgat ggctaagcga acccggcgtt aatttcatgc aaggctactg gattagtcgc
    12961 ccgttaccgc tggacgattt tgttcgctgg ctgaagaaac cgtatacgcc gcagtggtaa
    13021 ggtgtgctta cgtcccttat tattcatagt gaaagcatgc cggattgcgg ctaatgatga
    13081 gtaaaaggaa atccgttgca gatgattgtg cgcatactgc tgctgtttat cgccctgttc
    13141 accttcggtg cgcaggcgca ggctatcaag gaaagctatg cctttgccgt gctgggcgaa
    13201 ccccggtacg catttaattt caaccatttt gattatgtga accccgccgc gccaaaaggt
    13261 gggcagataa cgttgtcagc cctcggtacc ttcgataatt tcaaccgcta tgcactgcgc
    13321 ggcaacccgg gcgcacgcac cgagcagttg tacgacacgc tatttacgac ttccgatgac
    13381 gaaccaggca gttattaccc gctgattgct gaaagtgcac gctatgctga cgattattcc
    13441 tgggtggagg tcgctattaa tccgcgcgcc cgttttcatg atggttcgcc cattactgcc
    13501 cgcgatgtag agtttacttt tcaaaaattt atgaccgaag gcgtgccgca atttcgtctg
    13561 gtctacaaag gcaccaccgt caaagccatt gcaccgttaa ccgtgcgcat tgagttagct
    13621 aaacccggca aagaagatat gctgagtctg ttttcgctgc cggtatttcc agaaaagtac
    13681 tggaaggatc acaaacttag cgacccgctc gccacgcctc cgcttgccag tggtccgtac
    13741 cgcgttacgt cctggaaaat ggggcaaaat attgtctatt cccgtgtgaa agattactgg
    13801 gcagcaaact taccggtaaa ccgtggacgc tggaatttcg acaccattcg ctacgattat
    13861 tacctcgatg ataatgtcgc ctttgaagcg tttaaagcag gtgcctttga tttgcgtatg
    13921 gaaaacgacg ctaaaaactg ggccacgcgt tataccggta aaaatttcga taaaaaatac
    13981 atcatcaaag atgagcaaaa gaacgaatca gcccaggata cgcgctggct ggcgtttaat
    14041 atccaacgtc cggtattcag cgatcgccgg gtccgggaag caatcactct cgcctttgac
    14101 tttgaatgga tgaacaaggc gttgttttac aacgcctgga gtcgcacgaa cagttatttt
    14161 cagaataccg aatacgcggc cagaaattac cccgacgccg cggagctggt gcttctggca
    14221 ccaatgaaaa aagatctacc gccagaagtc ttcacacaaa tctaccagcc gccggtatct
    14281 aaaggcgatg gctacgatcg tgacaacctg ttaaaagccg acacacttct caacgaagcg
    14341 ggctgggtgc tgaagggtca gcaacgcgtt aatgccacaa cgggtcagca actcagcttt
    14401 gaattattgc ttcccgcaag cagcaatagt cagtgggtat tgccgttcca gcacagcctg
    14461 caacggctgg gtatcaacat ggacattcgc aaggtggata actcgcaaat caccaaccgc
    14521 atgcgcagtc gcgactatga catgatgccg cgcctatggc gggcgatgcc gtggcccagt
    14581 tccgatttac agatttcctg gtcatcggaa tatatcaatt ccacttataa tgcccccggc
    14641 gtgcaaagcc cggttatcga ctcactgatc aaccaaatta ttgccgcgca gggaaataaa
    14701 gaaaaattat tgccgttggg gcgagcactg gatcgcgtat taacgtggaa ttattacatg
    14761 ctgccaatgt ggtacatggc ggaagaccgt ctcgcctggt gggataaatt ctcccacccc
    14821 gctgtacgcc ctgtttacag cctgggtatc gatacctggt ggtatgacgt taataaagcg
    14881 gccaaactgc cgtcagccaa gcaacaggga gagtagatgg gcgcttacct gattcgccgt
    14941 ctgttgctgg tgatcccaac attatgggcg attatcacca tcaacttttt catcgtgcaa
    15001 attgcgcctg gcggtccggt cgaccaggcc atcgccgcca ttgagtttgg taatgccgga
    15061 gtattacccg gcgcaggcgg tgaaggtgtt cgtgccagcc atgcgcaaac gggtgtcggc
    15121 aatatcagcg acagtaatta ccgtggcgga cgcggattag atccagaagt gatcgctgag
    15181 atcactcatc gctacggttt tgataagccg atccacgaac gttacttcaa aatgctctgg
    15241 gactacatcc gctttgattt tggtgatagc ctgtttcgca gcgcctcggt gctgacgctg
    15301 attaaagaca gtctgccggt ttccatcacc ctcggattgt ggagcacgct gattatctat
    15361 ctggtgtcga ttccgttagg cattcgcaaa gctgttcata atgggagccg ctttgacgtc
    15421 tggagtagcg catttatcat tatcggctac gccattccgg cctttttgtt tgccatcctg
    15481 ctgattgtct tcttcgcggg cggcagctat ttcgacctgt tccctctacg cggcctggtt
    15541 tccgctaact ttgattcgct gccgtggtat cagaaaatca ccgattatct gtggcatatc
    15601 acgctgccgg tgctggcgac ggtgattggt ggctttgcgg cgctgaccat actgacaaaa
    15661 aactcattcc ttgatgaagt gcgcaagcaa tacgtggtga ccgcgcgtgc gaaaggggta
    15721 agtgaaaaaa atattctctg gaaacatgtg ttccgcaacg ccatgctgct ggtgattgcc
    15781 ggttttccgg cgacgtttat cagcatgttt tttaccggct cgctgctgat tgaggtgatg
    15841 ttttcactca atggtctggg cttactgggc tacgaagcga ccgtctcgcg cgattatcct
    15901 gtaatgtttg gtaccttgta tattttcacc ctgattggcc tgctgctgaa tattgtcagt
    15961 gatatcagct atacgctggt tgatccgcgt attgattttg agggacgtta atgtcgcgac
    16021 tcagccccgt caatcaggcc cgttgggcgc gttttcgtca taaccgtcgc ggctactggt
    16081 cgttatggat tttcctcgtc ttgtttggtt tgagtttgtg ttcagagctt atcgccaacg
    16141 ataaaccatt gctggtacgt tatgacggca gttggtattt cccgttgttg aaaaactaca
    16201 gcgaaagcga ttttggcggc ccgctggcaa gtcaggctga ttatcaggac ccgtggctga
    16261 aacaacggct ggaaaataac ggctgggtac tgtgggcacc gattcgcttt ggtgctacca
    16321 gtatcaactt tgctaccgat aagcccttcc cttctccacc ctcccggcaa aactggctgg
    16381 gaacggatgc caacggcggc gatgtgctgg cacgcattct ctatggcacg cggatctcgg
    16441 ttctgtttgg cctgatgctg actctctgtt ccagcgtgat gggcgtgctg gcgggggcgc
    16501 tacaaggcta ttacggcggt aaagtcgatc tctggggaca acgctttatt gaagtatggt
    16561 cggggatgcc gacgctgttt ttgattattt tactttccag cgtcgtacag cctaacttct
    16621 ggtggctgct ggcaattact gtcttgtttg gctggatgag tctggtcggc gtggtgcggg
    16681 cggagttttt acgtactcgt aatttcgact acattcgtgc ggcgcaggcg cttggcgtca
    16741 gcgatcgcag tatcatcctg cgtcatatgt tgcctaatgc catggtcgcg accctcacct
    16801 ttttaccgtt tattttatgt agttcgatca ccaccctgac ctcactcgat ttcctcggct
    16861 tcggtctgcc gctcggttca ccgtcactcg gtgaactgct gttacaaggg aaaaataacc
    16921 ttcaggctcc gtggcttggg atcaccgcct tcttgtcggt ggcgatatta ttgtctttgc
    16981 tgatctttat tggtgaagcc gtccgcgacg catttgatcc taataaggcg gtgtagcatg
    17041 acgcaaactc tgttagcgat tgaagatttg tcggtgggtt ttcgccatca gcaaaccgta
    17101 cgtacagtag tcaatgatgt ttcactacag attgaggctg gcgaaacgct ggcgctggtg
    17161 ggtgagtcag gttcaggcaa aagcgttacc gcgctgtcaa ttttacgcct gctcccttcc
    17221 ccgccggttg aatatctctc cggcgatatt cgttttcgtg gcgaatcgct gcttcatgcc
    17281 agcgatcaaa cgttgcgcgg tgtacgcggt aataagatcg ccatgatttt tcaagagccg
    17341 atggtgtcgt taaatccatt gcataccctg gaaaaacagc tttatgaagt gctttcactc
    17401 caccgcggga tgcgtcggga agcggctcga ggcgaaattc ttaactgcct tgatcgcgtt
    17461 ggtatccgcc aggcggcaaa acggctgaca gattatccgc atcagctctc cggcggcgaa
    17521 cgccagcggg tgatgattgc gatggcgctg ttaacgcgac cggaattatt aattgccgat
    17581 gaaccgacca ccgcactgga cgtctctgtc caggcgcaga ttttacagct gttgcgcgaa
    17641 ctgcaaggcg agctgaatat gggcatgctg tttattactc ataacctcag cattgtcaga
    17701 aaactggccc accgcgtggc ggtaatgcaa aacggtcgct gtgtcgagca aaattacgcc
    17761 gctacgctat ttgcctcacc cactcatcct tacacacaaa agctactcaa cagtgaaccg
    17821 tcaggcgatc cagtgccgtt gccagaacct gcctcaacgt tgctggatgt tgaacagctt
    17881 caggttgcct tccccattcg caaagggatt ttgaagcgca ttgtggatca taatgtggtg
    17941 gtgaaaaaca tcagttttac gctacgagcg ggtgaaacac tgggtttagt gggcgagtcc
    18001 ggttccggga aaagtacgac gggactggcg ctgctgcgac tgattaattc tcagggcagc
    18061 atcatctttg acggtcagcc actgcaaaat ttaaatcgcc gccagctgtt acctattcgt
    18121 catcgcattc aggtggtatt tcaggatcca aactcctcgc tcaacccacg actcaacgtt
    18181 ttgcagatta ttgaggaagg cttacgggtt caccagccga cgctttctgc cgcacaacgc
    18241 gaacaacaag tgatagccgt gatgcatgaa gtgggattag atcctgaaac acgccaccgt
    18301 tatccggcgg agttctctgg tggtcagcga caacgtattg caattgccag ggcattaatt
    18361 cttaagccct cgctgatcat acttgatgaa ccgacatcat cactcgacaa aacggtacag
    18421 gcgcaaatat tgacgctatt gaaatcattg caacaaaagc tgcaactggc ctatttgttt
    18481 atcagccacg atttgcacgt tgtccgcgcg ttatgtcatc aggttatcgt actgcgacaa
    18541 ggggaagtag tggaacaagg accgtgcgcg cgcgtgtttg ccgcaccgca gcaggagtat
    18601 acgcgtcagc tactggcgtt gagctgacgc ttaaaaagga ttgtagtttg aaaagggttc
    18661 ggcaattgcc acaccaaaat ttttcaatcg gcatgttgca gcaaactcgt cctggcgatt
    18721 tacaaacagg cacggctcac cttcacactc cagcaccgaa cacggaactt cgatgtcgct
    18781 taacgcctgg cttagtttgt acatcaccga ccatgcatta tcaccatccg ggctaagcaa
    18841 tttaagcgcc accaggctgt tgtcaccgca cggtccattt tgcggaatcg gttcaacctt
    18901 cgaacctgcg aaaccagcag accagcgaaa gttctgcggt agacgatgga cgattgagag
    18961 ttgtaatgaa gtcacttatt ttccccggaa gcacatttac ttcacaattt gtttacatca
    19021 attttaacac atcatcaaca atccgtcttt atacagatcg taaagattga tgcctcgcct
    19081 gcgtggcctc tatggctctg atttaagtaa tatctgcggg cgtggtcacg tttgcggggc
    19141 tatatgtacc cgtttctgcg atctaactca acctttttaa ctacaatgat gtgacttttt
    19201 acataaattg attttacata aaataaacat atatcggggg aatgatagat ttgtggttga
    19261 catgcatcaa caaaggaagc cttttagctt cctcgttgtg caatagatca ccgttttttc
    19321 ggtcgactgg cgtacagaca gaagagaatg gagctgcttg cgcagaatgc aattgaccaa
    19381 atcatcggcc atgcagagtt aaaggtcgca agagaaagca atgcgccaac aattgcccct
    19441 atgccaaaac ggaagattcc tgccagcgaa gatgccgttc ccgccatatg gggaaactca
    19501 tcaagaatga ccgccatcgc attggatgac accatcgaca cacagcccac aaacgccgca
    19561 acgccaacca ccagaatccc agccccagca gcgcactgat gaccatccac gctgccataa
    19621 taaattgtat ccacaacccc gagcggaaca tatttaacgc gccaatgcgg cgaacgaagc
    19681 ggctgttaaa gatggtcatc acgaacagga aaacaatgtt tagtgcaaag taataaccaa
    19741 agttttccgg cgcgacgtgg ttaatttcaa tgtagacaaa cggtccggcg ctcaggaacg
    19801 agaacattcc ggcaaagctg aaaccgctgg ccagcatata actcaggaca cgcttatgac
    19861 ggaacagcgc cgcaaagtta ccaatagtag tacgaatgtg aaaaggctgg cgacgctccg
    19921 gtggtaaggt ttctttgatc agaaagaaga tcattgccga agccagaatc gccgccacgg
    19981 cgaggatcca gaagatgtaa tgccagctca gccacaccag cacccagccg ccaactaccg
    20041 gtgccatcag cggtgcaatg gtcgtcacca gcatgacaaa cgacatcatc cgcgagaact
    20101 cttctttcgg gtagatatca cgcatcaaag cgttgataac catgctggcc gcagccgcag
    20161 ccagcccgtg gaaaaaacgc atcacaatca gctgatcgat ggtttgtgcc aacgcacacg
    20221 ccaccgcggc ggcggcaaac accagcgtac cgccgagcac caccggctta cgcccgaagc
    20281 tgtctgccat cggcccgtag attaactgcc ccaacgcaaa gcccagaata taagtactga
    20341 gggtcatctg cgtactgccc gccggtacgc caaactgcgc tgaaattacc ggtagcgcgg
    20401 gcagatacat atcaatcgac agcggcatca acatggccag caggccaagg ataaaaacaa
    20461 tagcaaacga cgaatgctgt cgggtggtca caacgggctc ctgaaagtca ttgaaaagtt
    20521 agacgacgct ggcaatttct tcttcagtta acggacgata ttcaccgggg gctaaatcag
    20581 catccagcgt aataccgcca atacgttcac gatgcagctc aaccacgtgg ttacccacgg
    20641 cggcgaacat gcgtttcacc tgatgataac gcccttcgct gatggtcaga cgaacctgcg
    20701 ttggggtaat cacttccagc accgcaggct tagtgagatc tttttcgtta tgcagctgca
    20761 cgcctttagc aaattgctct gccgtatcgt cagctacagg tgattccagt gtcaccagat
    20821 aggtcttctc gcaatgatgg cgcggagaag taatgcggtg cgaccactga ccatcatcag
    20881 tcatcagcac cagaccggtg gtatcgatat ccagacgtcc tgcggcgtgc agtttccagg
    20941 ctaccggttc atccagaaaa tagagcaccg ttgggtgatc agggtcgtcc gtggagcaaa
    21001 catagccctg aggcttattg agcatgaagt aacgtggacc atgttgctgc gccagcgggt
    21061 tgccatcgta agcgacatca tgttcaggaa gcagtttgaa cgctgcatta cgaacaattt
    21121 cgccatcgac ggtgacacga ttgccgcgga tttcacgccc ggcaatagca cggctaacgc
    21181 cgagttgctg tgcgataaat ttatcaagtc gcatgtgtgt gattttgcct gtaaaaacgg
    21241 aggtcgggca ttgacccgaa aatctgaact gttgtctgcc cagtatagcg gtctaataac
    21301 atccctcaag ggaaaaagat ccatggcata ctattagcag aataatctac ctacgcgaga
    21361 ccatgatttt tacactccgc ccatatcagc aagaagccgt ggatgccacg ctcaaccatt
    21421 ttcgtcgtca taaaacccct gccgttatcg tgctgcccac cggcgcaggt aaaagcctgg
    21481 tgattgccga actggcacgg ctggcgcgtg gtcgtgttct ggtgctggca cacgtcaaag
    21541 aactggtggc gcaaaaccat gcaaagtatc aggcgctggg gctggaagcc gatatttttg
    21601 ccgctgggtt aaagcgcaaa gagagccacg gtaaagtggt atttggcagc gtgcagtcgg
    21661 tcgctcgtaa tcttgatgcc tttcagggtg aattttcgct gttgattgtc gatgaatgcc
    21721 accgtattgg tgacgatgaa gagagccagt atcagcaaat cctcactcac ctgactaaag
    21781 tgaatcccca cttacgcctg ctggggctga ctgccacgcc ttttcgattg ggcaaaggct
    21841 ggatctacca gttccattat cacggcatgg tacgcggcga tgagaaagcc cttttccgtg
    21901 actgcattta tgagctgccg ctgcgttata tgattaaaca cggctatctg acgccgccag
    21961 aacgactgga tatgccagta gtgcaatacg atttcagccg cttgcaggca cagagtaacg
    22021 ggctgttcag cgaagctgat ctcaaccgtg agctgaaaaa acagcaacgc attacgccac
    22081 atatcatcag ccagatcatg gagttcgccg aaaagcgcaa aggggtaatg attttcgccg
    22141 ccaccgttga acacgcaaaa gagattgtgg gattactgcc tgccgaagat gcagcactga
    22201 ttactggcga cacccccggc gctgagcgcg atgtgttaat tgaagatttt aaagcccagc
    22261 gttttcgcta tctggtcaac gtcgcggtac tgaccaccgg atttgacgcc ccgcacgtcg
    22321 atcttatcgc cattctgcgt cctaccgaat cagtgagtct ttaccaacaa attgtcgggc
    22381 gcggtctgcg tctcgctcct ggcaagactg attgcttaat tcttgattat gcgggtaatc
    22441 ctcacgacct ctacgcgccg gaagttggta caccgaaagg caaaagtgac aacgtcccgg
    22501 tacaggtttt ctgccctgcc tgcggttttg ccaacacctt ttgggggaaa acgaccgccg
    22561 acgggacatt gattgaacac tttggtcgtc gctgtcaggg atggtttgaa gatgacgacg
    22621 gccatcgcga acagtgtgac ttccgtttcc gttttaaaaa ttgcccgcaa tgtaacgcgg
    22681 aaaacgatat tgccgcccgc cgctgccgcg aatgtgacac ggtactggtt gatccggatg
    22741 atatgttaaa agcggcgcta cgactgaaag acgcgctggt attacgctgt agcggcatgt
    22801 ctttacagca tgggcacgac gagaaaggcg aatggttgaa aatcacctat tacgatgaag
    22861 acggcgcgga tgtgagtgag cgtttccgtc tgcaaacgcc cgcccagcga actgccttcg
    22921 agcagctttt tatccgcccg catacgcgca caccgggcat ccctctgcgc tggatcaccg
    22981 ccgccgatat cctcgcccag caagccttac tgcgacatcc ggattttgtc gtcgcccgca
    23041 tgaaaggtca gtactggcag gtgcgtgaaa aagtgttcga ttacgaaggt cgttttcgtc
    23101 gggcgcacga attacgcggt taatggtagt tttgattgat gtataaggcg attgagtata
    23161 gaatctcgcc cgcttttgca tacgcaaagc agatcactta cctgttgctg ggtcgcctgt
    23221 agcaggatta atttaagaga gaaagaaatg tttactatca acgcagaagt acgtaaagag
    23281 cagggtaagg gtgcgagccg ccgcctgcgt gccgctaaca agttcccggc aatcatctac
    23341 ggtggcaaag aagcaccgct ggctatcgag ctggatcacg acaaagtcat gaacatgcaa
    23401 gctaaagctg aattctacag cgaagttctg gccatcgttg ttgacggtaa agaaatcaaa
    23461 gttaaagctc aggacgtaca gcgtcacccg tacaaaccga agctgcagca catcgacttc
    23521 gttcgcgctt aattgctgaa taagttgtaa aaaaccccgc tccggcgggg ttttttgtat
    23581 ctgcagatta tgcctgatta cggtattgct attttttgca ggccagataa ggcgttcacg
    23641 ccgcatctgg cattcagaat acaaaaccga ttaattgccg ccagacgtcc ggcgctgcaa
    23701 ttggtcgcgc aaattcggcg gcgtgccttt aatggtcaag gtatcggttg ctggatccca
    23761 gaagatgcgt tcacctaaca gcatcgcatc aaagttaata gtcaagccac cgccgctacc
    23821 ggcaaacttc gtcaactgac gcagtgtgct acggtctgcc gggaagctct cttccagttc
    23881 gtagcctttt tcagccgcaa acttcgtgaa gctcacttcg cttacgcctg ccagctcttt
    23941 cgacagcgat tccagttcga tctcttcgcc tgcctgcagc tgctcattac agtagctata
    24001 aacctgctgg cgcacgttct gtcgctctgc tttatccagt tgtgcttcgg cagtgaaatc
    24061 atcaaccgct tgcaacagcc cacggttttg cgctttcgcg ttcaatcctt cgctggcccc
    24121 gaggaaatcc ataaagaaat ccgccacttt gcgccctact cgccctttca ggaaagtgag
    24181 atagcgggtg gactctggat tggtttccca ttcggttaaa tcgatacgcg caacaatatc
    24241 cgcatgattg atatcaagat aatgggttgg gttgatgtcg agattttcgt taacgcgcat
    24301 actgctcagg ttgctcagaa ctgccaccag cagatactcc accgccagat aacggtagtg
    24361 gcaaaacagc acgaatccac cgtcagcaaa aggatatttc gctaattcgt cacgcaggcg
    24421 cccggttgcc gcgcggctaa atgccagaaa gtcctcttcg ccctgacgct gtaagcgcag
    24481 cgtctgcgcc agttcactct cttcgctaaa caagccgtaa gctttatttt tggcgctata
    24541 gacccgatgc agttctgcca ccatctcaac gacggtttct gtcggttcca gcaatgaatc
    24601 gcgcaacacc agctcaaggt tctgctcatc acgcttgata agctggtgca gagcaatctg
    24661 gttgatatcc agactcatga taaactctcc tttaagaccg ggcggtattc aaccaccgcc
    24721 ggttaagcga cgcaataaaa tatggcggtc attttacaga aggcagcgta acgagaatga
    24781 tttaacgcat aaaaaagcag aaaaaaaacc gttgctacgg taatatgttg ccctttcatc
    24841 aactaactga tttcgattta tgccacaaat ttcccgctac agtgatgaac aggttgaaca
    24901 attgctcgca gagctgctca acgtactgga aaaacataag gctccgaccg acctttccct
    24961 gatggtgtta ggcaacatgg tcactaacct tatcaacacc agcattgccc cggcccaacg
    25021 ccaggcaatt gccaactctt ttgcccgcgc cttacagtcc tctatcaacg aagacaaagc
    25081 gcactaaggg aaacagataa caggttatgg taactcatcg tcagcgctac cgtgaaaaag
    25141 tctcccagat ggtcagttgg gggcactggt ttgcactgtt caatattctg ctttcgctcg
    25201 tcattggcag ccgttacctg tttatcgccg actggccgac aacgcttgct ggtcgcattt
    25261 attcctacgt aagcattatc ggccatttca gcttcctggt gttcgccacc tacttgctga
    25321 tcctcttccc gctgaccttt atcgtcggct cccagaggct gatgaggttt ttgtccgtca
    25381 ttctggcaac ggcgggaatg acgctattac tgatcgatag cgaagtcttt actcgtttcc
    25441 atctccatct taatcccatc gtctggcagt tggttatcaa cccagacgaa aatgagatgg
    25501 cgcgcgactg gcagctgatg ttcatcagcg tgccggttat tttattgctt gaactggtgt
    25561 ttgcgacgtg gagctggcaa aagctgcgca gcctgacgcg tcgtcgacgc ttcgcgcgcc
    25621 cgctggccgc attcttattt atcgccttta tcgcctcgca tgtggtgtat atctgggccg
    25681 atgccaactt ctatcgcccg atcaccatgc agcgcgctaa cctgccgctt tcgtacccga
    25741 tgacggcgcg acgtttcctt gagaagcatg gcctgcttga tgcgcaggag tatcaacgcc
    25801 gtcttattga gcaaggtaat ccagacgccg tttccgtaca gtatccgtta agcgaactgc
    25861 gctatcgcga tatgggcacc gggcagaatg tgctgttgat tactgtcgat ggcctgaact
    25921 actcacgctt cgagaagcag atgcctgcgc tggcaggttt tgctgagcaa aatatttcgt
    25981 tcacgcgcca tatgagctcc ggcaacacta cagacaacgg catctttggc ctgttctatg
    26041 gcatctcgcc gagctatatg gacggcattc tgtcgacccg tacccctgcg gcgttaatta
    26101 ctgcgcttaa tcagcaaggc tatcagctgg gattattctc gtcagatggc tttaccagtc
    26161 cgctgtatcg ccaggcattg ttgtcagatt tctcgatgcc gagcgtacgc ccccaatccg
    26221 acgagcagac cgccacgcag tggatcaact ggctgggccg ctacgcacaa gaagataacc
    26281 gctggttctc gtgggtctct ttcaatggca ctaacattga cgacagcaat cagcaggcat
    26341 ttgcacggaa atatagccgg gcggcaggca atgtcgatga ccagatcaac cgcgtgctca
    26401 atgcactgct tgattctggc aaactggaca atacggttgt gattatcact gccggtcggg
    26461 gtattccgct gagcgaagac gaagaaacct ttgactggtc ccacggtcat ctgcaggtgc
    26521 cattagtgat tcactggcca ggcacgccgg cgcagcgtat taatgcgctg actgatcata
    26581 ccgatctgat gacgacgctg atgcaacgcc tgctacatgt cagcacacct gccagcgaat
    26641 attcgcaagg tcaggatttg ttcaaccctc aacgccgtca ttactgggtc actgcagcgg
    26701 ataacgatac gctggcaatt accaccccga aaaagacgct ggtgctgaac aataacggta
    26761 aataccgcac ttacaactta cgtggtgaaa gagtgaaaga tgaaaaacca cagttaagtt
    26821 tgttattgca agtgctgaca gacgagaagc gttttatcgc taactgatta attataaatc
    26881 agttagcgaa atatcttact tgcaatcggt gtggaaaacg gtagtattag cagccacgag
    26941 tcggcacgta gcgcagcctg gtagcgcacc gtcatggggt gtcgggggtc ggaggttcaa
    27001 atcctctcgt gccgaccaaa tcgcccaccg gataccaccg gaattaacag tttttagaca
    27061 cgaaaagccc gctaatcctt gattagcggg tttttttatg gtttttagtc accggacatc
    27121 accggattta taagctatca gccggatttt ttagttatac gcttagttat acgggcttgc
    27181 gtataactaa agccgtataa ctaaaaccct gttttttcgt gtggaatcat atacatatgg
    27241 cgagaaaaac acccccatta accacggtgc agatcaaagc agccagacca gcggaaaagg
    27301 aatacaccct acaggacggc ggagggcttt ttctcctggt caaaccgtcc ggatcaaaac
    27361 tctggcgatt ttcctactac cggccatcgg acaaaaaaag aatattgctg agttttggat
    27421 cgcttgatga tgtttccctg gctgatgcca gaaaacgccg tagcgagtac agagcgttaa
    27481 tcagtgccgg aactgacccg cagggccatg agaagaaaaa acgagaggca gaggcccgaa
    27541 gacaagggaa cacgttcgaa aatgtggcgg cggcatggta ccaggtgaaa atcagccaga
    27601 atctggcccc caacacgatt aaagacatct ggcgttcgct ggataaatat gtattcccgt
    27661 tcatcggcaa cacgccaata gataccctca ccgcccgaag gttcgttgaa gtgcttacac
    27721 ccatcaagga gcgcggcaac ctggaaacac tcaagcgggt tttacagcgc gttaatgagg
    27781 taatggatta cgccgccaac agtgggctga ttgatgccaa tccggctatg aatgtgcgta
    27841 aggcgtttcc ctcacctgta aaaaaacata tgccaacaat ccgccccgaa cagctgccgg
    27901 agcttatgca ggctttatca gtatcggcaa cagaacggca gaccagatta ctgattgaat
    27961 ggcagttact gaccgtaacc cgtcccgccg aagcgtcatc aacgcggtgg gatgaaatca
    28021 acctggacgc gaagcaatgg acgatacctg ccggacgcat gaagatgcgc agggatcacg
    28081 ttatcccgct ttccggtcag gctatggcgg tgctggaggc catgaaacca atcagccacc
    28141 accgcaatta cgttttccca agtctgaaag acccacagca gccgatgaac agccagacag
    28201 ctaacgcagc attgcggcgt atgggattcg ctggcgtgct ggtgtctcat ggattacgcg
    28261 ccatattcag cacagcagcg aacgaggaag gattcgagcc ggacgtaata gaggcggcac
    28321 ttgcccacgt cgacaccaac gaagttagac gggcatacaa ccggagcaac tacatagaaa
    28381 aacgcatcgt gctgatgcgc tggtggggcg aatttgtcga ggctgcggcg acgggcgtaa
    28441 ccctcgccag tggtaaaagg ggtatccgag ccgtgtagct gtacagaaaa ccagtaaaaa
    28501 ctacgaaaac catgtaaaac cgtcgtataa ttgcatcaaa tttaacgaca aggccgtgaa
    28561 acatgaaacc gttaagatgc aaaaaaatat cagatgcaat tgcgacgggc tgcaactggc
    28621 cctgatggtt cagcatgaat tttggtcaac ctacgatccg gaggacagaa cgacggcccc
    28681 atcaaaaaaa caggtagtag attttctggt atcccgtggc gcttccagaa atctggcggt
    28741 aagtattgat aaggtcgtac gtccggcatc tatgaagatc ggaggcaggc ccaaaaaatg
    28801 gcggtaacaa tcctggaagc ggcagaaatg ccgctttttt tataattcca tttcaaatca
    28861 tcaatataaa aacggtgtat accgtttaaa aacggtggga actgttttta cccatatccg
    28921 atgatttacc gtatttgtca ccggaataca ccggattcac aaggtaaatc acgatggaag
    28981 caatcagaaa aattatcttt cgccaggaag taaaaaaaat tatccacatc aaggcagaca
    29041 gcacgctgca aagcatgatc aacgccggag aatttccgca gggttttcgc gttggtttac
    29101 gccgtcgcgg atggtatgag gatgatgtgt tggcctggca gaaagaacgc gaacaggaag
    29161 cacgcggaac ggctgcttaa cgggtatcac agatatgaca aacacgaaaa aagcggcccc
    29221 gatatggagc cgcctttctg aacaattaac ccgctgcgcc ttatgtgtat atgatcccaa
    29281 acataagcac ggggatgata gccgctatca ggctggtggg caatgcaatc agtctggttc
    29341 agttcgttgc catacctgca atgagcgctt ttccctatac tctttaagga attgctcaag
    29401 ggcaaaagca catggtgcga atctttctga ttcatgctct atctttctgc gccgtctttt
    29461 ccgtgccggt gataatgttt tggtcaattc tttatcggtc attgtgttgt cctgcatagc
    29521 aatgcgccgt aatacctcac accacggcgc tgatagtttt tatcctttgg gttctatgcc
    29581 gcgccgcttt agttcagtgc gccccaactc tttaagccag ttggctaggc ttatgccgtc
    29641 gctctgtgct tctttgtcga attgctcttt tagctctgga gaaattcgca ttctgaattg
    29701 tggggattgc ccgtcccctt tagggctttt atcgcgtttg attattgaca agtggtcacc
    29761 tattgaatta gcctttccat tgttaggtgg ccaccttaac acaagagcac ttaaaaaagc
    29821 aaagccccgc aagtgtcatt accactcgca gggcttctaa ccaacaacgt aaactaggag
    29881 ccgttatggt tgccgtaaat catataccac accttgtaca cacacaaacg gcctttgtgt
    29941 ggcgttttct ggccctgagt gccggagaat ctcaaatcat ccacgtaacc gcctggacgg
    30001 aacgcgaagc gcgtagccgt tgcccgtccg gttgtgttgc tgtattcgcc gcccgtattc
    30061 gtcaggggga aacctatgca caataaaacc acaccgaacg cagcagccgc cgcactcact
    30121 acgctgatgc acgcgcttat tgatattgaa tgcactgcag agcttgcgca gggagaagaa
    30181 cagaaagacc ggacacagtt cgccctggaa tgtatccgat acatcgcaac gcggtcgctg
    30241 aatgacgcta aaaatattct tgttgctgat tgtgaaaatg gggggggtta tgcgtgatga
    30301 tcgttttaat tccctgaaac aggaatttcc cggcgttcct gatgatgcgg ctgatgcgct
    30361 ttcgtcaatg ccagaactta ttagagcggc ttttttctta ctttccacga gagaatataa
    30421 atcaacgggg cttgatgtac tgaatatcgc cgccgattat gcggaatatg tggcagaggc
    30481 gcgttacaga agaaaatttc ctgaggatgt aagccatgcg tgatatttac cacgaaacaa
    30541 tagaccgcgc atttcttgca ctttctcaca gtgaaaacat gctggaaata ttgcgcatat
    30601 ggcttgaaac acttggcgac aatgaacgcg acaaacaaaa atcaagaatt gccacggcat
    30661 taataacgct tcttgagcct gtaataatgg aactgcaaga aatagatcta ttgcacgaca
    30721 gatataaaga acagcacacc ggagaataaa aataatgaaa cttaaatatt ctggcttaac
    30781 tgccagtggc aacactcacc ctaaatttac gcgcggtgat atttaccgcg accagtacgg
    30841 cggcacggta atgattaagg gcgtggaaga acggcgtgta acctaccgcc gtgaaggtta
    30901 cgaatatgat tgcgtgatgc ctgtttatca gttccggcgt gatttttctc tggtacagac
    30961 cgcgccgcat aacgtgccca ccagcaacgc cagggcacgg gcaaacatcc agaagctgaa
    31021 aaccatgatt aacggattca ggggcaagaa atgaaaagcg caccgaactt aaaaaaacag
    31081 ccttacgaca agatgaccga agtcattatt tttgcgggta gtgatgcctg ggcacatgca
    31141 aaacagtggc aggaacagga cgggcgactg gctggcgata atgtgcctcc cgttgtgctg
    31201 gctgatgatc aactggatga actggcagac ctgagaatca tcgacgaggg gcgctattgt
    31261 gtccggctgt acaaggcagg ccacatcagg ccatcaaata ttaatgccat tgcgcacaag
    31321 ctggcggcgg cgggtgtaac tgatgcgaat tattaccccg aagggatgca cagccatatg
    31381 cgggagaact ggcgcgaata cctggaacgg gtgcgcggga aagagccggt ggaagaaaaa
    31441 aaccaccagc gaaaaaccac gctaccgatg agcgttggat ctaccggata cgacacgcaa
    31501 ctggattacg tggttaaggg gattattccg gcggtatcgc tatgcagcat atacggggct
    31561 agcgggtcct ataaatcatt ccttgccgga tcgtgggcgt gccatgttgc cactggtcgc
    31621 cagtggggag gccgcagggt tgcacatggt gcggttctct atgtggttgg tgaaggcggt
    31681 atcggcgttc cgcgtcgtgt aaaagcctgg gaggttgtgc acgatgagca ggtgaaaaat
    31741 ctgtatctgg taaaccgccc catctttccg gctgccccgc ttgatgttga tgaaatggtt
    31801 atcgctgccc gtcaggtgga gcgggaaacg ggtaaacctg tacgcatgat tattctggat
    31861 acgctggcgc gttgctttgg tgggaatgat gaaaatgatt cccgtgatat gggggcgttt
    31921 atccgtggtt gtgacgaact gaaacgacgc acaggggcca cggtgctggt ggttcaccat
    31981 tccggcaagg atgagacgaa aggcgcgcgc ggttccagtg catttcgtgc ttcgctggat
    32041 gctgaatacc ggatacgcag ggaggacgca ggaagcaaag cgctggttat ctcatgcacc
    32101 aaaatgaagg acgcggagga actcaaagaa gccgcatatg acttacgcgt ggtggagctt
    32161 tttaccgacg ctgacggtga attaatcacg tcgctggtgg tggtggatga tccgcgccct
    32221 cctgttgaac tggagcgcat cgaggaggca gggaacaaga cggaaaacca taccgcgcta
    32281 tgggggtgca tccgttcacg cacacagaac ggcgacaagt gcacgatccc gctgttacgt
    32341 gatgacatga aaaagctggg gtatgaaatg aaaaacttcc ggcgctggct gtacaagctg
    32401 gaaaaagatg gggttattag tatcgatggg gatgatgtag cgccgctata aaagtgagga
    32461 gcaaaagcga gggggataga aagagggcca aaattagccc gctctccctc acttttcgac
    32521 ctgtatacat cctcaaaagt gaggggtaaa aaaatactta taaaacacac acatagaaaa
    32581 accgaaaatc ccaactgcga cgaagtgaga cgcttgaaaa agtgaggcga aaaagtgaga
    32641 ggttgcgaga aatgacccaa aaacgcagag acagaacaga gccaaaatat aaagcgttag
    32701 acatgactga gcacacctta aaggtggcaa tcagaacgat agaccgccac acgcgggaag
    32761 gatacgcgaa ggaacatccc gacctgataa gcgcattcat gaccacggcg gcggcaaact
    32821 ttgccacgct gacagaacgg gagattgccg aagcggaaca ggtaacaacc atcaacgtta
    32881 aaaccggaga ggtggaatta tgacggcaca gatagccgct tacgggcggc tggtggacga
    32941 cccgcaggta aaacagacca gcaagggcac accgatgacg ctggcgcgta tggcggtatc
    33001 tttgccatgc agccaggcac aggacggaca ggcgacgtta tggttatcgg tcatcgcatt
    33061 tggcaaacag gccgacttcc tggctaaaca tcaaaaaggc gatgttgcca gcgtatccgg
    33121 cacgatgcag gtaagccagt ggaccggaca gaacggagaa acgcggcagg gctggcaggt
    33181 tatcgcagac agcgtaatca gtgcccgcgc ggcacgtcca ggcgggaaca gacgcaaaac
    33241 cacaggcaca cagggtaatc agccaccagc gggaggcgat gacccttacg gtgatgatat
    33301 ttcgttctga gggggtgacg atggtacatg accgcatagc ggaggaactg gaggcgaaag
    33361 gcttttaccg gagggcggcg gcgcgatggg gtgaagtcat gcagctggtg gagacagaca
    33421 aggaacggca tcacatcacg atgcgacggc tggaatgttc aaggaaggca cagagggcac
    33481 cggagccgcc ggataatttc ggagacctga aaaaggcagt cgatcgcact tatgccgaaa
    33541 tgggtataga tggtgctggt gatgaaatat ggcgcaatta ccaggacagc taatcaaaca
    33601 gccggagaaa tccggctttt ttgcaccagt tgaaacggta tggcgcatta ccgggttttc
    33661 gtcacggtca ggcatagtta ctatctgaaa caaacagaca caacagagga aaaaaacaat
    33721 gccgatgaaa tttgatgaga tattaaaaca gcgtgataaa taccatgctg acaacatgga
    33781 gacgatgagc atcaatgatt accgcgcatt cctggagacg ggcgcactga ttgaaaagga
    33841 tcagcatggt tttgtgagat gtgctctatc cggtgaaatg ctggcggtaa atcctgaaca
    33901 gatagatgca ttgatagaat ttctgaaaga gatcagagac tgatccagca cacagcacac
    33961 atagccggag caatccggct tttttgcgcc caaaaaaagc ccgataaggt cagagggttc
    34021 ttatcgggct tttgcatatg aggtttttgg atgcactggc gttcgtgatc gggataatca
    34081 tttcataatt tgcaacataa ctcaatattt ttgcataaaa tgcaattctg attataatca
    34141 ggactggata aacatccagt cctgattttt ttagtcgaag aggaatttct tactatggct
    34201 gaagagaaaa aaggcggtgt ttcggtgtac ataagccccg aaatcgtgga ggtgctcaag
    34261 cagcgccaca aaaaaaacta tgaggctggc gtggcggctg gactggatcc gctgatgacg
    34321 ccggagccgt cgataggttc acttgtacgc tcttatttac ttgcggcgct tgggatgcat
    34381 aaaaattatg ggggtgaata atggcaggca aagcaacggc acttaacact aaccagcttt
    34441 ttatgtacct gaatcgcggg gatattgcgg attttaaatt cagccctctg tttaccacgc
    34501 tgtttttccc gaacgtggcg acattcagca cgcaaaacat catgctggat accctggaca
    34561 ttgaagaagt caccatgtcg gcgttttgtt cgcctatggt gggtagccag gttcagcgcg
    34621 ataaagggta cgaaaccagc acaatcaaac ctggctacat gaagccaaag cacgaaatcg
    34681 atccaacgaa aaccatcatg cgcatggctg gagaagatcc ggcacagctt aacgacccta
    34741 cctatcgccg tatgcgcctg attactggca acatgcgccg ccagataaac gccattaagg
    34801 cacgcgtgga atggctggcg gtgaatgcgg taacgaccgg aaaaaacatc attgagggcg
    34861 aaggcataga acgctatgaa atcgactgga agataccgga aaaaaacatc atagagcagt
    34921 ccgacggcaa aaaatggtcc gagcatgata aagatattta cgatccaatc tatgacatcg
    34981 aactatacgc agatcaggca ggttgccccg ccaacgtcat gattatgggc gctgaggtat
    35041 ggcgcacgtt acgcagcttt aaaaaattcc gtgaactgta cgatctttcc cgtggttcag
    35101 aatccgccgc agaaatggcc tgtaaaaacc tgggcgaagt ggtgagcttt aaaggctatc
    35161 tgggcgatat tgctcttatt gtctattccg gcaaatacac tgacagcgac ggcaccgaaa
    35221 aatatttcct tgagcctgat ttgctggtcc tgggcaacac caacaataaa gggctggtgg
    35281 cctatggtgc gattatggat caggaagcgg taagaacggg cgcaacgcaa aacatgtact
    35341 acccgaaaaa ctggattgag gacggcgatc cggcgattga gtacgtgcag acacacagcg
    35401 caccgcagcc ggttccggca gatattcgca aatttgttac cgtcaaaatt ggttaacggg
    35461 ggattatatg gacactccat atattgagtt atttgcaggc agtcagcagg tatcaacgac
    35521 actggtacat tttgccgctg atgctggcgt tattcaggaa tttaccccgc tgatgctggt
    35581 ggacaatggc gagtttaagc cgtgggatgg tcaggaatct ggcaaggctg tttatctgac
    35641 ttcgtacctc gtggacacgt caaagcagaa atcagcacag tgttacaaga cggggatatt
    35701 caatattgcc gccgttaact ggcctgagag cgtcgacacc gatgcgaaaa aatgcgccgc
    35761 ctttgcgggt tctggcgtat ccgttcagcc gctggcccga taagcagggg gaacgatggc
    35821 aacgaatgaa agcatcatgg cgctaccgct ggcgagtaaa tttaaagccg aagcgcgggc
    35881 aatggctgac agaggtttat caacctacga ggccgtatat caactcaaca aactggaaga
    35941 gcaggacaag ccgcgcgctg atgcgattat ggcgcttcat gagcataaag actatcagcc
    36001 gctgttgcgt gcaatggcaa acgtgccttg tatcgatgtt gatacggcta aaaacatcct
    36061 gaacatgacc atagagcagg aacgcccgaa ggttgcacca gagcttaccg cagcctttga
    36121 aaactttatg gacatgcaca gcccacaaac cgtatcagct ggcatggcat acgatggcag
    36181 aaacccaggc gatgacggcg acatcgatcg catactgaaa accatctgag acaaggccgg
    36241 agaaatccgg cttttttgcg ggtccttcct ggaattatgg cccgttacgg ggcggcgacc
    36301 tcgcgggttt tcgctattta tgacgttttt ccgtgaaggt gacaccacca ccacttgatt
    36361 aatatttaac catgcagtta aggtaacatt atgattgata aagcttgttt tgtaagtcag
    36421 caggaaatag ctgaacattt caaggttaac agaaccacta ttcgcgcatg gaccaaacag
    36481 gggatgccgt atcttaatgc ggatcgcgga aagtctggcg gttatcacat cgggcataca
    36541 ttgctttggt cttcaggtaa aagccgtctt gaggccatca gatatcacgt agaaaccagt
    36601 gcgctggaaa aaattatgtt tgccaggctg ctttcatctg agcgtgacga gtactccagt
    36661 gaagaaacag aacatcgatt tgatgaaggt ttgcagattt acggctattc accggaagat
    36721 gtgagcaagg cacgaaataa aatggctggc tttctggctg ggtggcgtca tgccgtaagc
    36781 gttcgccgtg ccagcatgga gcaatcagcc gatacagaac agtaaagccg ataacttgtt
    36841 aatcactcgc agcaatgcat gtgatttgtg cacactttcg gttaactggt tcgatgtccg
    36901 gttttagtgt ctgttttttg cgcatgtccg gttcatggaa agcatgtttt tatatttttc
    36961 atatggttaa cttgtagaga aaccggacat ggatcccgaa aaattttcat aaatagtgaa
    37021 aacgcgcgag gtcgccgccc cgtaacgggt ccataattcc aggaaggacc cgacgacacc
    37081 agacaatcag aacgataggt gcacaatgac agaagccgaa atactgggat taatccgccg
    37141 tgctggtgga atcagccagc agactgacga acaggccacg cagccggaca gcgtgacagc
    37201 cgaaaattat gcgcgtgttg ttgctgaggt gatgcgccgt gatggtatcc agcttaatga
    37261 tgtggatatg cgcaacatac ggatccgcgt tcttgaaatg ctggcctaca atcgccgcgt
    37321 ggcactgtat cgggaaacgg agaaaattac ataccactgg aagaagccgg agcggttgcg
    37381 gcggtaactg gttgatattt tcgaaattcg ccagcggcga acatcgggga aaccagccta
    37441 acccgttgat attttcggga acctcaaatt gaggaggtcg gaaacataac ccgtcatatt
    37501 ctggatgctg ataattacca cgcagcccat taaggcctaa cccgttgatt ctttcggaaa
    37561 cagccattgg cgtaatccgg cgcggtaact tgctgaactt aaagcaaagc gcaaaattgc
    37621 gctggctggt gggcatgatg ccgatatggt gatccccata acggaaatat ccaccagcac
    37681 ggcaacggca gaatagccac aacacagaaa aaccacgaat atggggtttt tgttatggca
    37741 tgggcatgat gaccgcccct gctctgaatg gtggaaatca ccacgcagat catccaccag
    37801 cttcgttttt ataaattaaa catccgggaa aatccgaccc tgaattgtgc attttttcag
    37861 caaatcattt tagttatacg tttggttata cggtttaaaa gttgaatcat atacattatt
    37921 ttaaatcatg aagttacacg ctcaattaaa ctactctcgt gaaaaatccc aagaaaaaac
    37981 caacctttac ggttggtttt tttatatctg caattaattt gataaacaga ccgtgacaca
    38041 tcacagcctg tttattttct gttatcagaa cgtccagacc actcccgcct gagtattcca
    38101 cggcgattct acccctgcgc cattgccata gctgactgac aaatgaccgc ttaacgtcgg
    38161 ggtaaacgat gaccttatcc cagcctgata aacgccaggg gttccagcca catcattatt
    38221 aaatttgcca tcgtcattaa ctttcacatg gttagaatcg gcgtattcct gacgtacggc
    38281 ggctttcagc cagggttcca gcgtcgtacc attttgcagg tccatgtgat agcttaccgc
    38341 cgttcccgct tcagcgcgta atatccgggt atttcccaca tcagcgcgca tgccgtttga
    38401 taacgtgtag tcctgaccat ctgtggtaaa gccggtaaag gccagatagg gtctaacact
    38461 ccacaatccg tcaacccaac ggaacccgct ctcaacatga gcacccgcgc cgttactatt
    38521 gtaatcgcca aacgctgttg ccccattact catcttgcca tggatggtgt tggcaaaacg
    38581 gtcaactttc accaccccat caacataggc accgttctga tgctcccaac cggcataagc
    38641 ccccagggta tagctatcga tattaccttt tccgccgcga tcaaaaccaa tatcagaatg
    38701 agagtaacca aagaacaagc cgcgaattgt actgctttct tcacgggaga aacggctatc
    38761 gataccgagc gtcaggcccg tcaatgtttg ctcaaaacca gctcccgcat cagtggtcac
    38821 gttgttgcgg gtgttaattg ccgaactcca catcgccgta tcgtaactaa cgctttttac
    38881 gctaccaaga cgctcacgca cggtgtccag ttctgcatca aataccagcg gttgtgcggc
    38941 cgccatattc agcacatcag tggttgaagg ggtaatttgc gcgcgattct ctgccaaact
    39001 ccagctatgg ttgccattat ccagcaaggt atattcatac gtaccgatat caacaacgcc
    39061 tccggcattg cccaacgtaa atgcagcatc accgccgccc gttgttacca gtgtaaggct
    39121 atctcctgct gccgggctgg caccggtgtc cgtcacgaat attttgaaat cacctgttgc
    39181 ctgaccggtg acgttgagct gatcgctctg atgattagcc atatcggtac gcatataaaa
    39241 attgccgttt cccgacaggg tattggtggt caacgtaatg aaattttccg cacttgccgt
    39301 cactgatgaa cgagtcactg gctcatccat cataatcaca tttgcgtcag acatcgcaag
    39361 gttggctaag ttgaggaggg tgttttggtt cgtcgtagtg atatcggcaa tgatggccca
    39421 tacgctattt tcgagcgaaa cgtccgcttt gctggtatcc acggcaccat gcgttctaaa
    39481 agaagcgccc tcagaaacca ctaccttacc caccaacata gcgggtgcat aatttatttg
    39541 cggcttcatg acctcaagaa tgccatcatt ccctctgact gacacgttaa ccattgtgcc
    39601 agcccagaca ttagcgcggc cattgttgat aaccatattt tcagagatgg ctttagagga
    39661 atacgtgata cttccattct ggtctgatcg accgagatca tataccgcat ttgcctgcac
    39721 tttcgtacca gtatcctttc ccagcgattg catagtggca tgctcatcaa ggatagtatc
    39781 aatggcctgc gtgtcctcca taacaatgag cccggaacca tcatccagtt cataattttt
    39841 tgacacacca tcttttatac taaattggcc tttactgttt gtaccactca cttccagcgc
    39901 attcgttgag acaattaatt ttccgcctgc ttttttatca acgccagttg cagtcccgcc
    39961 atccataacc tccagtaagc cgccactatc tacagtagta ttataagcga agtcattttc
    40021 ttcaactcgc aaacttccgc cgttttccaa cagcatattg ttagcaatac cattcttgat
    40081 ttcgaactga ccgagacggt ttattccgaa tgcctccatg acccgcgtgc ttgctttaat
    40141 agcaccgcct gctttctgat ctaccgcaat cgctaatccc ccctctttaa cctccagtaa
    40201 tcctcccttt tcgatagcgg caaggacggt aatcccatct ttttcaacga ttaagcgccc
    40261 gcctttaatc gtggg
//
LOCUS       DAZPEB010000013        39667 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZPEB010000013 DAZPEB010000000
VERSION     DAZPEB010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39667
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.013"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(13..870)
                     /gene="murI"
                     /locus_tag="ACHURJ_000645"
     CDS             complement(13..870)
                     /gene="murI"
                     /locus_tag="ACHURJ_000645"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFR1279714.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
     gene            complement(815..2663)
                     /gene="btuB"
                     /locus_tag="ACHURJ_000646"
                     /pseudo
     CDS             complement(815..2663)
                     /gene="btuB"
                     /locus_tag="ACHURJ_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
     regulatory      complement(2717..2907)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3021..4121
                     /gene="trmA"
                     /locus_tag="ACHURJ_000647"
     CDS             3021..4121
                     /gene="trmA"
                     /locus_tag="ACHURJ_000647"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFR1279715.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPCILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     gene            complement(4161..4520)
                     /locus_tag="ACHURJ_000648"
     CDS             complement(4161..4520)
                     /locus_tag="ACHURJ_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFR1279716.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4520..5170)
                     /gene="fabR"
                     /locus_tag="ACHURJ_000649"
     CDS             complement(4520..5170)
                     /gene="fabR"
                     /locus_tag="ACHURJ_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFR1279717.1"
                     /translation="MMGIRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            5491..6891
                     /gene="sthA"
                     /locus_tag="ACHURJ_000650"
     CDS             5491..6891
                     /gene="sthA"
                     /locus_tag="ACHURJ_000650"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFR1279718.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLVLDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            complement(6874..7791)
                     /gene="oxyR"
                     /locus_tag="ACHURJ_000651"
     CDS             complement(6874..7791)
                     /gene="oxyR"
                     /locus_tag="ACHURJ_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFR1279719.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(8058..9431)
                     /gene="argH"
                     /locus_tag="ACHURJ_000652"
     CDS             complement(8058..9431)
                     /gene="argH"
                     /locus_tag="ACHURJ_000652"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFR1279720.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDAFDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSHVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            complement(9492..10268)
                     /gene="argB"
                     /locus_tag="ACHURJ_000653"
     CDS             complement(9492..10268)
                     /gene="argB"
                     /locus_tag="ACHURJ_000653"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFR1279721.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            complement(10276..11280)
                     /gene="argC"
                     /locus_tag="ACHURJ_000654"
     CDS             complement(10276..11280)
                     /gene="argC"
                     /locus_tag="ACHURJ_000654"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFR1279722.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPAINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            11434..12585
                     /gene="argE"
                     /locus_tag="ACHURJ_000655"
     CDS             11434..12585
                     /gene="argE"
                     /locus_tag="ACHURJ_000655"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFR1279723.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDIDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVIERWPGRLTVDELHPPIPGHECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            12937..15588
                     /gene="ppc"
                     /locus_tag="ACHURJ_000656"
     CDS             12937..15588
                     /gene="ppc"
                     /locus_tag="ACHURJ_000656"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFR1279724.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEAPPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYKSWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIR
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDVYRGYVRENKDFVPYL
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            15771..17504
                     /locus_tag="ACHURJ_000657"
     CDS             15771..17504
                     /locus_tag="ACHURJ_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFR1279725.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            17719..18528
                     /locus_tag="ACHURJ_000658"
     CDS             17719..18528
                     /locus_tag="ACHURJ_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR1279726.1"
                     /translation="MYHDISYLLSRLINGPVPDLAYQVDFPRLEIVLEGEFVDTGAGA
                     TLVPGDVLYVAAGGWNFPQWKTPTTTFSVLFGKQQLGFSVVQWDGKQYQNLAKQHVAR
                     RGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQIQTASRSQALFEAI
                     RDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYLNHTRLEHAKTLLKG
                     YDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLTEKPTTPE"
     gene            complement(18515..18856)
                     /locus_tag="ACHURJ_000659"
     CDS             complement(18515..18856)
                     /locus_tag="ACHURJ_000659"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFR1279727.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(18858..19736)
                     /locus_tag="ACHURJ_000660"
     CDS             complement(18858..19736)
                     /locus_tag="ACHURJ_000660"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418387.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFR1279728.1"
                     /translation="MTSSAGQRISCNVVETRLNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLNIRQIHLLPFHQYGEPKYRLLGKTWSMKDVAAPSSADVAT
                     MREMAERAGFQVTVGG"
     gene            complement(19702..21999)
                     /locus_tag="ACHURJ_000661"
     CDS             complement(19702..21999)
                     /locus_tag="ACHURJ_000661"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFR1279729.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAVLAETMAANCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDEQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLWEAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(22050..22370)
                     /locus_tag="ACHURJ_000662"
     CDS             complement(22050..22370)
                     /locus_tag="ACHURJ_000662"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFR1279730.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEARESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(22385..23464)
                     /locus_tag="ACHURJ_000663"
     CDS             complement(22385..23464)
                     /locus_tag="ACHURJ_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFR1279731.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVVI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            23773..26274
                     /gene="ptsP"
                     /locus_tag="ACHURJ_000664"
     CDS             23773..26274
                     /gene="ptsP"
                     /locus_tag="ACHURJ_000664"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFR1279732.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLRQHLLTGVSEGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMTQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            26286..26948
                     /gene="fsa"
                     /locus_tag="ACHURJ_000665"
     CDS             26286..26948
                     /gene="fsa"
                     /locus_tag="ACHURJ_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFR1279733.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            26959..28062
                     /gene="gldA"
                     /locus_tag="ACHURJ_000666"
     CDS             26959..28062
                     /gene="gldA"
                     /locus_tag="ACHURJ_000666"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFR1279734.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAAFSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYATLLVADQYGQRFLQEWE"
     gene            28336..28953
                     /locus_tag="ACHURJ_000667"
     CDS             28336..28953
                     /locus_tag="ACHURJ_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFR1279735.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGNTLFYDPAYVRLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWKLKRPDSNIDHRRVPNLETWFSRHNKTRPISKNAGDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(28980..29885)
                     /gene="yijE"
                     /locus_tag="ACHURJ_000668"
     CDS             complement(28980..29885)
                     /gene="yijE"
                     /locus_tag="ACHURJ_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFR1279736.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMHPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYTRHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(29979..32159)
                     /gene="katG"
                     /locus_tag="ACHURJ_000669"
     CDS             complement(29979..32159)
                     /gene="katG"
                     /locus_tag="ACHURJ_000669"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFR1279737.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     SSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(32488..33378)
                     /gene="metF"
                     /locus_tag="ACHURJ_000670"
     CDS             complement(32488..33378)
                     /gene="metF"
                     /locus_tag="ACHURJ_000670"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFR1279738.1"
                     /translation="MSFFHASQRDALNQRLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            33454..33588
                     /locus_tag="ACHURJ_000671"
     CDS             33454..33588
                     /locus_tag="ACHURJ_000671"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1279739.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            complement(33727..36159)
                     /gene="metL"
                     /locus_tag="ACHURJ_000672"
     CDS             complement(33727..36159)
                     /gene="metL"
                     /locus_tag="ACHURJ_000672"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFR1279740.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(36162..37322)
                     /gene="metB"
                     /locus_tag="ACHURJ_000673"
     CDS             complement(36162..37322)
                     /gene="metB"
                     /locus_tag="ACHURJ_000673"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFR1279741.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWTNNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            37599..37916
                     /gene="metJ"
                     /locus_tag="ACHURJ_000674"
     CDS             37599..37916
                     /gene="metJ"
                     /locus_tag="ACHURJ_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFR1279742.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            38100..38708
                     /locus_tag="ACHURJ_000675"
     CDS             38100..38708
                     /locus_tag="ACHURJ_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFR1279743.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTVKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEPQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            38853..39528
                     /locus_tag="ACHURJ_000676"
                     /pseudo
     CDS             38853..39528
                     /locus_tag="ACHURJ_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         9731 a        10961 c         9947 g         9028 t
ORIGIN      
        1 cattcaacca aatcagccta aaactgccag tttttcgagc gtttcgaagc cgtaacgctg
       61 taaaacgggc aataattgtt cagcttctgg cgtcattgcc atacaaaagg caatattcgc
      121 atcggcagat tttgcatccg gggcttcatg ttctaacaac caggccgttc ggcgagcaat
      181 cgctgcgcca gaatccacta gccgggttcc ctctggcagc acttgtaaca gttcttcttg
      241 tagtagaggg aaatgggtgc aacccaatac aacggtatct ggcggctctt tcattcttaa
      301 ccacgggcgt aggatacgtt ttagtgcatc cagagaaaca tcttcgccat gtagcttcgc
      361 ttcagccaac tcaaccatct ctgccgagcc cagcatttct atctggcatt cattagcgaa
      421 acgcgcgatc agctcatgag tataagaacg tttaactgtt ccgcgggttg ccagtaatcc
      481 gacaatgcca tttgccgtca gacgtgcagc aggtttaatc gccggcacga caccaacaac
      541 cgggaagtcg aacttttcgc gtaatgcagg aagtgaaacg gtactggcag tgttgcaagc
      601 gaccacagcc agcgcaaggg gataacgttc ttgcaccgcg gtgacaattg ccaccactcg
      661 ctcaacaata aacgcttcgc ttttttcgcc atacgggaaa gcgacgttat cgaaagcata
      721 aatgtaatgg agatccggta agagatgccg gatctcgtca tagaccgaca acccaccgac
      781 gccggagtca aacaccagca cggtgggacg tggttcagaa ggtgtagctg ccagacaagg
      841 tgtattcccg tcctgcagtt tggtagccat agactgtctc ataatctttg tcgaacaggt
      901 tggctatttt accacgaact gtcaggtgag aggtgaccgg atacgcaacc gcaagatccc
      961 acaagctcac accgcccatt ttaacggttt gataaggata agatgagtaa tccttatcat
     1021 agcgagtgcc taaatactga taagtaatac cccagtcgaa gtcatacaac tgccagtcga
     1081 gctggtattt cacctgctgt ttagcacggc gtaacaacgg cgtgtcggta atcgcattgc
     1141 gcgcatcgac ataatcataa ctcacagtat gcgtcagtgg tccggtatca aaattggcgg
     1201 tcgcctcgac gcccttaatc cgcgctttcc cttcgttgta atatttcagg gtgtgatcat
     1261 cataatcgat caagtcactg acatcgttac gatatccgga aatacgccag ttcaccccag
     1321 cggttaagcc ttcaaacgcg ccttcccact gtttgctttt ctccgggtcc agattcggat
     1381 ttccgtagaa gccatacagt tgccccagat ttggtgcctt ataagatgtc ccgtaggaag
     1441 caatgaagcg ataaccttcg atgaattccc aaccggcgct ggtttgccag gttccatgac
     1501 gaccaaactg tgagttatcg tcactgcgtg ctgcgccttc aaaggtaaaa tcgccgactt
     1561 gttgcagccc ggtcagatag atgccggtat tacgttgatc atatccatcc tcaacataac
     1621 ctgtacccgg cgtcgtagtc tgtttctgcc agtcgacacc cgcaccaata ctaccgtgac
     1681 caacgatgac attgtttgcc cactggacgg tgtattgctt catctcatcg agcgtcgccg
     1741 acgaatcata acgaccataa tggggatcgt agttgtaatc tttgctatgg ctatagctgg
     1801 taatgagttg tgatttaatc agttcgccgt tatagcgcag cccggcgtcc caactttggc
     1861 tatagagttt acggatatcg agcaacggtg aaccgggaga ataatacgcg tcataattgg
     1921 tacggttatc atagccatag ccgcgcacaa agccgctcca ggcatcagta aagttatgct
     1981 ccagcgcgcc ataaagcgtt ttacttaaaa aaacatcgtt atctgtctgc gcttgcgttc
     2041 cggtattacc ataggcaaca acatcataac catgagtatg ggcataatcg cccaacagcg
     2101 ttacccgtgt cttatccccc agttgttgct gcgtagagac atcatagttc tgataactat
     2161 tgcttcccca ccctgctgaa atttccgttc cgggttcatc gcgcgtcgtg atgatattca
     2221 ccaccccgcc tattgcatcg gaaccataaa cagcggagcg cggcccacgg atatattcaa
     2281 cacgctggac aagcgcacgc aatagggaac tggctaaggt cggcagaacc actcaccccc
     2341 gccagattca ggcgtacgcc atcaattaac accaacacat gactggcatt tgtaccgcga
     2401 ataaaaatag atgagagctg acctgaaccg ccgttttggg tgatatcgac gcccggaaga
     2461 cggcgcagca catcattgac cgaggtcgac tgccagcggt cgatatcctg acgggtcaca
     2521 acggtggttg gtgcaagcac agtgctgcgc ggctgttcaa aacggttagc agtaacgacg
     2581 agagtatccg ggctggtatc ctgtgcccaa gcggaaaagg ctgtgacaga acacgccgtc
     2641 agcagcgaag cttttttaat cattgtaaag catccacaat agaagaagga tgccgcaggt
     2701 ttcatcaata ttacgcgatg atgagacgtt ggccggcagg tcttcgggct tggaggggta
     2761 tctaagatac taagagatga tgacttccca ccggatggca gtgtccgcat aacgcaatca
     2821 tcgcaccttt ccttaccgct gcgcgtcagc tccagattcg cactggattc cctattaact
     2881 cacaggaccg gcaagtggat gctacaggtt gtaacaagtt actgtccaga cgtagctcac
     2941 aaataggaat tcatcaagat ctggacatct gatgagcaat ccctacaatc gccgcgtact
     3001 ttaatttttc aggatacatc atgacccccg aacaccttcc aacagaacag tatgaagcgc
     3061 agttagccga aaaagtggta cgtttgcaaa gtatgatggc accgttttct gacctggttc
     3121 cggaagtgtt tcgctcgccg gtcagtcatt accggatgcg tgcggagttc cgcatctggc
     3181 acgatggcga tgacctgtat cacatcattt tcgatcaaca aaccaaaagc cgtatccgcg
     3241 tggatagctt ccccgccgcc agtgaactta tcaaccagtt gatgacggcg atgattgcgg
     3301 gtgtgcgtaa taatcccgtt ctgcgccaca agttgttcca gattgattac ctcaccacgc
     3361 tgagtaatca ggcggtggtt tccctgctat accataagaa gctggatgat gagtggcgtc
     3421 aggaagcgga ggccctgcgc gatgcactgc gcgcgcagaa tctgaatgtg catctgattg
     3481 gtcgggcaac gaaaaccaaa atcgagctgg atcaagatta catcgacgag cgtctgccgg
     3541 tcgcagggaa agagatgatc taccgtcagg tagaaaacag ctttacccag ccgaacgcgg
     3601 cgatgaatat tcagatgctg gaatgggcgc tggacgtaac caaaggctca aaaggcgatt
     3661 tactggagct gtactgcggc aacggtaact tttcattagc gctggcgcgc aattttgatc
     3721 gggtattagc caccgaaatc gctaagccgt cggttgctgc tgcgcaatac aacatcgcag
     3781 ctaaccatat tgataacgta caaattattc gtatggcggc agaagaattt actcaggcga
     3841 tgaatggtgt gcgcgagttt aaccgcctgc aagggatcga cttaaagagt tatcagtgcg
     3901 aaaccatttt tgtcgaccca ccgcgcagcg gtctggacag tgaaaccgag aaaatggtgc
     3961 aggcgtatcc gtgtattttg tacatctcct gtaacccgga aacgttatgc aagaatctgg
     4021 aaacattaag ccagacgcac aaggtcgaac gtctggctct gtttgatcag ttcccctaca
     4081 cgcaccatat ggagtgcggc gtattactga ccgcgaagta aaacccatgc cggatgcgcc
     4141 agcatccggc ataataccga ttactcagca acttcctgct tacggttacg catcttcgcg
     4201 ccaatccaga acaccataat gacggagagt actgccggga agaagttaga gccgatatcc
     4261 ggatattccg cacgaactac cgtgctgtac agcagtacgc cgagaataaa acaggcagct
     4321 gccagacccg gcaagcctac cggcatagtg cgattaagat aacgttgatg caggcagtaa
     4381 accgtcagca ccagggaaat aatcgggaat acagaaaatg gcacaatgga gctaaacagc
     4441 gctgcgaaag taccattaat cgataagcca gcaactaacg ccagcagcag cgtacctcta
     4501 tcttgatttg cttgtttcat tactcgtcct tcacatttcc cggaataatt gcggttttct
     4561 cttgttcacg gcgataccag taataagccc ctttcgaaat cattcgcagt tgcagtacca
     4621 gtcgctcttc taattgccga cgttgttcga cgccgacgtc caacgcctcg gcacccgcac
     4681 tgaagacaat tgtcaccatt gcttcggctt gcgcttcagt aaacgcacgc ggcatatggt
     4741 tttcgagttc cagatagtcc gcaagttccg caatgaagtg ctgaatttca cgcgcaacgg
     4801 cggcacgaaa cgcagcggag gtgccggagc gttcccgcaa taataaccgg aaggcgttag
     4861 gattattacc gatgaactcc ataaatgtgg agaccgaggt gcggatcaca ctcccgcctt
     4921 tggcgatacg ctgacgcgcc tggcgcatga gttggcgtag cattaaaccg ctctcatcaa
     4981 ccatggtcag acccagttcg tctacgtcgc ggaaatgccg ataaaaagag gtgggagcaa
     5041 tgcccgcttc acgcgccact tcacgcaaac tcaggctggc gaagctgcgt tcagcactta
     5101 attggctaaa tgcggcttcc accagcgaac ggcgggtttt ttctttttgt tgcgctctta
     5161 tgcccatcac gatgtctgaa tccttgccaa aagtactgct ggcactatac cagagaatga
     5221 acatactgga ttgctccagt attgtgaatg aacggtaacg caataaaaca tatttactgg
     5281 ccggttcttt tacgtacagc ggaaacctgc cgcttaaacg gagagtatcg tcgataaaaa
     5341 tccaataaaa cgtcagggca aaagtaagaa acagacaaag caaaggccgc tcaggatata
     5401 gccagataaa tgacggggat caattggctt acccgcgata aaatgttacc attctgttgc
     5461 ttttatgtat aagaacaggt aagccctacc atgccacatt cctacgatta cgatgccata
     5521 gtaataggtt ccggccccgg cggcgaaggc gctgcaatgg gcctggttaa gcaaggtgcg
     5581 cgcgtcgcag ttatcgagcg ttatcaaaat gttggcggcg gttgcaccca ctggggcacc
     5641 atcccgtcga aagctctccg tcacgccgtc agccgcatta tagaattcaa tcaaaaccca
     5701 ctatacagcg accattcccg actgctccgc tcttcttttg ccgatatcct taaccatgcc
     5761 gataacgtga ttaatcaaca aacgcgcatg cgtcagggat tttacgaacg taatcactgt
     5821 gaaatattgc aggggaacgc tcgctttgtt gacgagcata cgttggtgct ggattgcccg
     5881 gacggcagcg tcgaaacact aactgctgaa aaatttgtta ttgcctgcgg ctctcgtcca
     5941 tatcatccaa cagatgttga tttcacccat ccacgcattt acgacagcga ctcaattctt
     6001 agcatgcacc acgaaccgcg ccatgtactt atctatggtg ctggagtgat cggctgtgaa
     6061 tatgcgtcga tcttccgcgg tatggatgta aaagtggatc tgatcaacac ccgcgatcgc
     6121 ctgctggcat ttctcgatca agagatgtca gattctctct cctatcactt ctggaacagt
     6181 ggcgtagtga ttcgtcacaa cgaagagtac gagaagatcg aaggctgtga cgatggtgtg
     6241 atcatgcatt tgaagtcggg taaaaaactg aaagctgact gcctgctcta tgccaacggt
     6301 cgcaccggta ataccgattc gctggcgtta cagaacattg ggctggaaac tgacagccgc
     6361 ggacagctga aggtcaacag catgtatcag accgcacagc cgcacgttta cgcggtgggc
     6421 gacgtgattg gttatccgag cctggcatca gcggcctatg accaggggcg cattgccgcg
     6481 caggcgctgg taaaaggcga agccaccgca catctgattg aagatatccc taccggcatt
     6541 tacaccatcc cggaaatcag ctctgtgggc aaaaccgaac agcagctgac cgcaatgaaa
     6601 gtgccatatg aagtgggccg cgcccagttt aaacatctgg cacgcgcaca aatcgtcggc
     6661 atgaacgtgg gcacgctgaa aattttgttc catcgggaaa caaaagagat tctgggtatt
     6721 cactgctttg gcgagcgcgc tgccgaaatt attcatatcg gtcaggcgat tatggaacag
     6781 aaaggtggcg gcaacactat tgagtacttc gtcaacacca cctttaacta cccgacgatg
     6841 gcagaagcct atcgggtagc tgcgctaaat ggcttaaacc gcctgtttta acactttatc
     6901 gaaatggcca tccattcttg cgcggatggc ctctgccagc tgctcatagc ggctgcgcag
     6961 cggtgagcca ggacgataaa ccaggccaat cgtgcggcga ggttccggct taatacacgg
     7021 caaataaaca accccatcgc gtttgcgctc cggcggcaca gccagcgcag gcagtaaagt
     7081 gatcccgcta cctgccgcca ccatgttacg cagtgtctcc aggctggtcg cgcggaagtg
     7141 tgtatcttca tccgccccgg cttcaaagca gaaacccatt gcctgatcgc gcaaacagtg
     7201 accatcttcc agcatcagca gtttttcccc tgccagatcg gccatcggta cgcattcgcg
     7261 gttcgcccac gggtgatctt catagatagc cagcaacatt ggctcatcaa acaacggcac
     7321 ttcaatgaat gcttcgctct ctttcaccag cgcgaggatc acgcaatcga gtttgccgct
     7381 gtccagttgc gccagtaact ggtgggtctg tgcttcatgc agatacattt ccagctttgg
     7441 aaatgtctgg tgcagcatcg ggataatatg cggcagcagg tacggtccaa ccgtaggaat
     7501 caaaccaata tgcagcggtc cggacatcgt ctcgccctgc tggcttgcca tctctttaag
     7561 gactttcacc tcacgcagca cggtacgcgc ctgatccacc agcagcattc ccgcctgggt
     7621 gaacaacact ttacggctgg tccgctccag caacatcacg cccagctcat cttccagctt
     7681 acgaatttgg ccgctaagcg tcggctggct aacgtggcag gaatctgccg cacgccgaaa
     7741 atggcggtgt tcagccaatg ccaccaggta ctcaagatca cgaatattca ttatccatcc
     7801 tccatcgcca cgatagttca tggcgatagg tagaatagca atgaacgatt atccctatca
     7861 agcattctga ctgataattg ctcacagaaa cggagcggca cctcttttaa cccttgaagt
     7921 cactgcccgt ttcgagagtt tctcaactcg aataactaaa gccaacgtga acttttgcgg
     7981 atctccagga tccgcttttt tttgccataa aaaagcccgg cgataagcca ggctcaaatt
     8041 tgtacatata aatgttctta ccccaaccgc gcctgcgcaa aagcaatcgc ttgggccacc
     8101 tgctgcggtg agacgccgcc ttttgccgca cgcttgtcga ggcacgattg tagcgacaga
     8161 atcggataga catcttcgcc aatcacgtgg ctgaatttct gcaactcgtc gagcggcaga
     8221 tcttccagcg gtttgccctg acgaatggct tccaccaccg cttcacccac gatatggtgc
     8281 gcctcgcgga aaggtacgcc tttcgccacc aggtaatccg ccagttcggt ggcgttggcg
     8341 taaccctgct gagccgcttc ctggcaacgt ggacgtttca cctgaatgcc gtccagcacc
     8401 agcgccgcca tatgcaggca gtccagccag gtatcgaacg cgtcgaacag accttctttg
     8461 tcttcctgca tatctttgtt gtaagccagc ggcaaacctt tcagcgtcat catcatgccg
     8521 gttaacgcac cctgcacccg accgcattta ccgcgaatca gctccagcgc atccgggttt
     8581 ttcttctgcg gcattaatga tgaaccggaa gtcacgcggt cagaaagctc cacaaacccc
     8641 gcttcgccgg tgttaaagaa aatcagatct tcagcaaaac gcgacagatg caccatgccg
     8701 atagcggcag ccgacagcag ttccaacaca tggtcacggt cagaaacgct gtccagactg
     8761 ttacgggtcg ccgaagcaaa gcccagccag cctgctaact gttcacggtc gatttcatag
     8821 gccgttcccg ccagcgcgcc acagcctaat gggctgacat ccagacgctt aagcgcatcc
     8881 tgcaaacggc tttcatcacg cgccagcatc tcaacatagg ccaggcacca gtgcgcgaac
     8941 gtcaccggct gggcgcgttg caggtgagtg taacctggca ttaccgcgtc ctgattgttt
     9001 tgtgcggttt ccaccagcgc cgattgcagc tgccggttag ccgtcagtaa ctcgctaacg
     9061 gtatctttgc accacagttt caggtcagtc gctacctgat cattacggct acgcccggta
     9121 tgcagctttt tgcctaactg gcccactttg tcgatcagtt tgccttccac ccagctatgg
     9181 atatcttcgg cgtcgctttc aaggatttgt tgtggcctgg cgcgaacatc ttccagcaac
     9241 acgttcagcg cctcttccag ttgcgcctgc tcttctgcgg ttaacacgcc gaccgttacc
     9301 agggctttgg accaggccac agagccaaca atatcctgct ccgccagacg gtaatcaaag
     9361 cgcagtgagt cgttgaattg tttgaaccgt tgatctgctg cctgggtaaa acgcccgccc
     9421 caaagtgcca taactctgtt tccttatttt gaaattcaat gccggaaagc tgcgcctccg
     9481 gctaacaaaa cttaagctaa aatccgcgta cccatcggca taccgttaaa cagtgccgga
     9541 agctgctccg catgacgcca ggaggcgata tctaccggac ggcccagcgt gcgggccgca
     9601 tccagcgccg cgttcacttt cactatcatg ccgtcagtaa taatgccctg ctcaatcagt
     9661 tgttctgctt tcgcggcggt catttcggca atgcgttgcc ctttgccgtc gagaatgccg
     9721 ctgacgtcgg agagcaaaat cagatccgcg cccagcgttg ccgccagcgc cgttgccgcc
     9781 tggtcggcat tgacgttcat cagttgccct tcgtctgtta cgccaatgga gctgaccacc
     9841 ggcagataac cgttctccag caaggagttg ataagcttag gcgaacctgg ctgcgccagt
     9901 ccaacatgac ctaactcttc atcaagctgg gtcactttga cgctgtcgcc atcaccgaga
     9961 aacaaaccta cagccgcaat ctgatgtttc ttcgcccatg ccaacagggt tttatttgcc
    10021 gttcccgcca gtgctccggt gataatgtct atctgatcag caggcgtcac ccgcaggccg
    10081 tttttctttt tcaccggcag attcagccct ttcatcagct catccaccac gcaaccgccg
    10141 ccgtgcacaa tcaccagcgg acgctgatgt gactcacgat aattcaccag tgcgctaaac
    10201 agacgttcca gcgcctcttc actatccagc agtacgccgc ccagtttgat aattaatgga
    10261 ttcatcattg cacccttaaa taagagactg cgtttccgca tagccgaaac gaatattggc
    10321 gcactgtacc gcttgtgccg ccgcgccttt cagtaagttg tcttcggtcg ccacaataat
    10381 cagatgctcg ccctgaacgg caaacccgat atcgcaaaat ggcagcccaa cgacattttt
    10441 cagcgccgga acgcctttgt catacagccg caccagcggt ttatgggcat acgcctgctg
    10501 taacacttgc gcgacttgcg cctgggtcac acccgatttc aggcggcagg taatggtttc
    10561 gagaatgccg cgcgggaaat tgcccagatg tggggtgaag ataacgtcag caccgaggtg
    10621 tgtggcgatc tctggttgat ggcgatgagt aaagacgcca tacggttgca ggctaacttc
    10681 acaaaaacta tttgaaatcg ctgccttacg tcctgcaccg ctcacgccgc tggtggcgtt
    10741 aatcgccggc cactgattga ggtcaagaag atcggcatca atcaacggtt tcagcgccag
    10801 ctgtgccgcc gtcggataac agcctggcac cgcaatcaaa ttcgcttctt ttaatttatt
    10861 accgcaccac tccgccagac cgtaggctgc ctgttccagc agttccgggt attgatgggt
    10921 aaagccgtaa tatttttcat agaaggtggc gtcgttaaca cgaaacgcgc cggaaaggtc
    10981 gaacaccacg cagcccgctt caagaaactg cggcgctaaa tcgtggctaa cttcatgggc
    11041 ggtggcgaga aacactacgt ccacccctgg gctaaactcg ctgatatccg acatcggctg
    11101 caacggcagc tcaacgatgc cttttagctg cggatgcaaa tcggagatta actttcccgc
    11161 atcattgctt tgcgctgaaa cagtcaaagc ggttatgttc atatgcggat ggcgatttac
    11221 ataggtcact agctctgcgc cagcgtagcc gctggcaccc acaatcagcg tattcaacat
    11281 cggggctatt caccttctta tgtctggttg ccaggttaaa cgtaaaacat tcaccttacg
    11341 gctggtgggt tttattacgc tcaacgttag tgtattttta ttcataaata ctgcatgaat
    11401 attgatacta tcatgaccag aggtgtgtca acaatgaaaa acaaattacc gccatttatc
    11461 gagatttacc gcgctctgat tgccacacct tcaataagcg ctacggaaga ggcactcgat
    11521 caaagcaatg cagatttaat cactctgctg gcggactggt ttaaagattt gggcttcaat
    11581 gtggaagtgc agcctgttcc aggaactcgc aacaaattca atatgctggc aagtatcgga
    11641 cagggggctg gcggcttgtt gctggcgggg cataccgata cggtgccatt tgatgacggt
    11701 cgctggacgc gcgatccgtt tacactgacg gagcatgacg gcaagcttta cggcttaggc
    11761 accgccgaca tgaaaggctt ttttgcgttt atccttgatg cgctacgcga tatcgacgtc
    11821 acgaaactga aaaaaccgct ctacattctg gcgactgctg atgaagaaac cagtatggcc
    11881 ggagcgcgtt attttgccga aactaccgcc ctgcgcccgg attgcgccat cattggcgaa
    11941 ccgacgtcac tacaaccggt acgcgcacat aaaggtcata tctctaacgc catccgtatt
    12001 cagggccagt cggggcactc cagcgatcca gcacgcggag ttaacgctat cgaactaatg
    12061 cacgacgcca tcgggcatat tttgcaattg cgcgataacc tgaaagaacg ttatcactac
    12121 gaagcattta ccgtgccata ccctacgctc aacctcgggc atattcacgg tggcgacgct
    12181 tctaaccgta tttgcgcttg ctgtgagttg catatggata ttcgtccgct gcctggcatg
    12241 acactcaatg aacttaatgg tttgctcaac gatgcattgg ctccggtgat cgaacgctgg
    12301 ccgggtcgtc tgacggtcga cgagctgcat ccgccgatcc ctggccatga atgcccaccg
    12361 aatcatcaac tggttgaagt ggttgagaaa ttgctcggag caaaaaccga agtggtgaat
    12421 tactgtaccg aagcgccgtt tattcaaacg ttatgcccga cgctggtgct cggtccgggt
    12481 tccatcaatc aagctcatca gcctgatgaa tatctggaga cgcggtttat taaacccacc
    12541 cgtgagttga ttatccaggt aattcaccat ttttgctggc attaaaacgt aggccggata
    12601 aggcgctcgc gccgcatccg gcaccgttgc caaactccgg tgccgcaata atgtcggatg
    12661 cgatgcttgc gcatcttatc cgacctacac ctttggtgtt atgtatggcg attttttaac
    12721 atttccataa gttacgctta tttaaagcgt cgtgaattta atggcgtaaa ttcctgctat
    12781 ttattcgttt gctgaagcga tttcgcagca tttgacgtca ccgcttttac gtggctttat
    12841 aaaagacgac gaaaagcaaa gcccgagcat attcgcgcca atgcgacgtg aaggatacag
    12901 ggctatcaaa cgataagatg gggtgtctgg ggtaatatga acgaacaata ttccgcattg
    12961 cgtagtaatg tcagtatgct cggcaaagtg ctgggagaaa ccatcaagga tgcgttggga
    13021 gaacacattc ttgaacgcgt agaaactatc cgtaagttgt ccaaatcttc acgcgctggc
    13081 aatgatgcta accgccagga gttactcacc accttacaaa atttgtcgaa cgacgagctg
    13141 ctgcccgttg cgcgtgcgtt tagtcagttc ctgaacctgg ccaacaccgc cgagcaatac
    13201 cacagcattt cgccgaaagg cgaagctgcc agcaacccgg aagtgatcgc ccgcaccctg
    13261 cgtaaactga aaaaccagcc ggaactgagc gaagacacca tcaaaaaagc agtggaatcg
    13321 ctgtcgctgg aactggtcct cacggctcac cctaccgaaa ttacccgtcg tacactgatc
    13381 cacaaaatgg tggaagtgaa cgcctgctta aaacagctcg ataacaaaga tatcgctgac
    13441 tacgaacaca accagctgat gcgtcgcctg cgccagttaa tcgcccagtc atggcatacc
    13501 gatgaaatcc gtaagctgcg tccaagcccg gtagatgaag ccaaatgggg ctttgccgta
    13561 gtggaaaaca gcctgtggca aggcgtacca aattacctgc gcgaactgaa cgaacaactg
    13621 gaagagaacc tcggctacaa actgcccgtc gaatttgttc cggtccgttt tacttcgtgg
    13681 atgggcggcg accgcgacgg caacccgaac gtcactgccg atatcacccg ccacgtcctg
    13741 ctactcagcc gctggaaagc caccgatttg ttcctgaaag atattcaggt gctggtttct
    13801 gaactgtcga tggttgaagc gccccctgaa ctgctggcgc tggttggcga agaaggtgcc
    13861 gcagaaccgt atcgctatct gatgaaaaac ctgcgttctc gcctgatggc gacacaggca
    13921 tggctggaag cgcgcctgaa aggcgaagaa ctgccaaaac cagaaggcct gctgacacaa
    13981 aacgaagaac tgtgggaacc gctctacgct tgctaccagt cacttcaggc gtgtggcatg
    14041 ggtattatcg ccaacggcga tctgctcgac accctgcgcc gcgtgaaatg tttcggcgta
    14101 ccgctggtcc gtattgatat ccgtcaggag agcacccgcc ataccgaagc gctgggcgag
    14161 ctgacccgct acctcggtat cggcgactac aaaagctggt cagaggccga caaacaggcg
    14221 ttcctgatcc gcgaactgaa ctccaaacgt ccgcttctgc cgcgcaactg gcaaccaagc
    14281 gccgaaacgc gcgaagtgct cgatacctgc caggtgattg ccgaagcgcc gcaaggctcc
    14341 attgccgcct acgtgatctc gatggcgaaa acgccgtccg acgtactggc tgtccacctg
    14401 ctgctgaaag aagcgggtat cgggtttgcg atgccggttg ctccgctgtt tgaaaccctc
    14461 gatgacctga acaacgccaa cgatgtcatg acccagctgc tcaatatcga ctggtatcgc
    14521 ggcctgattc agggcaaaca gatggtgatg attggctatt ccgactcagc aaaagatgcg
    14581 ggcgtgatgg cagcgtcctg ggcgcaatat caggcacagg atgcattaat cagaacctgc
    14641 gaaaaagcgg gtattgagct gacgttgttc cacggtcgcg gcggttccat tggtcgcggc
    14701 ggtgcacctg cccatgcggc actgctgtca caaccgccag gaagcctgaa aggcggcctg
    14761 cgcgtaaccg aacagggcga gatgatccgc tttaaatatg gtctgccaga aatcaccgtc
    14821 agcagcctgt cgctttatac cggggcgatt ctggaagcca acctgctgcc accgccggag
    14881 ccgaaagaga gctggcgtcg cattatggat gaactgtcag tcatctcctg cgatgtctac
    14941 cgcggctacg tacgtgaaaa caaagatttt gtgccttact tgcgctccgc tacgccggaa
    15001 caagaactgg gcaaactgcc gttgggttca cgtccggcga aacgtcgccc aaccggcggc
    15061 gtcgagtcac tacgcgccat tccgtggatc ttcgcctgga cgcaaaaccg tctgatgctc
    15121 cccgcctggc tgggtgcagg tacggcgctg caaaaagtgg tcgaagacgg caaacagagc
    15181 gagctggagg ctatgtgccg cgattggcca ttcttctcga cgcgtctcgg catgctggag
    15241 atggtcttcg ccaaagcaga cctgtggctg gcggaatact atgaccaacg cctggtagac
    15301 aaagcactgt ggccgttagg taaagagtta cgcaatctgc aagaagaaga catcaaagtg
    15361 gtgctggcga ttgccaacga ttcccatctg atggccgatc tgccgtggat tgcagagtct
    15421 attcagctac ggaatattta caccgacccg ctgaacgtat tgcaggccga gttgctgcac
    15481 cgctcccgcc aggcagaaaa agaaggccag gaaccggatc ctcgcgtcga acaagcgtta
    15541 atggtcacta ttgccgggat tgcggcaggt atgcgtaata ccggctaatc ttcctcttct
    15601 gcaaaccctc gtgcttttgc gcgagggttt tctgaaatac ttctgttcta acaccctcgt
    15661 tttcaatata tttctgtctg cattttattc aaattctgaa tataccttca gatatcctta
    15721 aggaattgtc gttacattcg gcgatatttt tttcaagaca ggttcttact atgcattcca
    15781 cagaagtcca ggctaaaccc ctttttagct ggaaagccct gggttgggca ctgctctact
    15841 tttggttttt ctctactctg ctacaggcca ttatttacat cagtggttat agtggcacca
    15901 acggcattcg cgactcgctg ttattcagct cgctgtggtt gatcccggta ttcctctttc
    15961 cgaagcggat taaaattatt gccgcagtaa tcggcgtggt gctatgggcg gcctctctgg
    16021 cggcgctatg ctactacgtc atctacgggc aggagttctc gcagagcgtt ctgtttgtga
    16081 tgttcgaaac caacaccaac gaagccagcg agtatttaag ccagtatttc agcctgaaaa
    16141 ttgtgcttat cgcgctggcc tatacggcgg tggcagttct gctgtggaca cgcctgcgcc
    16201 cggtctatat tccaaagccg tggcgttatg ttgtctcttt tgccctgctt tatggcttga
    16261 ttctgcatcc gatcgccatg aatacgttta tcaaaaacaa gccgtttgag aaaacgttgg
    16321 ataacctggc ctcgcgtatg gagcctgccg caccgtggca attcctgacc ggctattatc
    16381 agtatcgtca gcaactaaac tcgctaacaa agttactgaa tgaaaataat gccttgccgc
    16441 cactggctaa tttcaaagat gaatcgggta acgaaccgcg cactttagtg ctggtgattg
    16501 gcgagtcgac ccagcgcgga cgcatgagtc tgtacggtta tccgcgtgaa accacgccgg
    16561 agctggatgc gctgcataaa accgatccga atctgaccgt gtttaataac gtagttacgt
    16621 ctcgtccgta caccattgaa atcctgcaac aggcgctgac ctttgccaat gaaaagaacc
    16681 cggatctgta tctgacgcag ccgtcgctga tgaacatgat gaaacaggcg ggttataaaa
    16741 ccttctggat caccaaccag cagacgatga ccgcccgcaa taccatgctg acggtatttt
    16801 cgcgccagac cgacaagcag tactacatga accagcaacg tacgcagagt gcgcgtgaat
    16861 acgacaccaa cgtgctgaag ccgttccagg atgtgctgaa tgaccctgcg ccgaagaaac
    16921 tgatcatcgt tcatctgctg ggtacgcata tcaaatacaa ataccgctac ccggaaaatc
    16981 agggcaagtt tgatggcaat accgatcatg ttccgccagg attaagcgca gaagagctgg
    17041 aatcatataa cgattatgac aacgctaact tgtataacga tcatgtggtt gccagcctga
    17101 ttaaagactt taaagcggca gacccgaacg gattcctggt ttacttctct gaccacggtg
    17161 aagaggttta cgacacgccg ccgcacaaaa ctcaggggcg taacgaagac aacccgacgc
    17221 gccacatgta caccattccg ttcctgctgt ggacgtcgga aaaatggcaa gcgactcatc
    17281 cccgtgattt ctcacaggat gtcgatcgta aatacagcct ggcggaactg atccacacct
    17341 ggtcagattt ggcgggctta tcttacgacg gttacgatcc aacccgttca gtggtgaatc
    17401 cgcagttcaa agaaactacc cgctggattg gtaacccgta caagaaaaac gcgctgatcg
    17461 attacgacac tctgccgtat ggcgaccagg taggtaatca gtaattatca gccaggcgct
    17521 atttgccgga tgcgacgctt gacgcgtctt atccggccta cagatcgcgg cggtaggctg
    17581 gataaggcgt ttacgccaca tccggcaata caactgccgc aatgtttaca tcgaactccc
    17641 ctcaggccac acttttcagg tggccttttt taataatgcc agttactgtt ttcccgtcta
    17701 catacctaag ggaataacat gtatcacgac atcagctatc tgctctcccg cctgatcaac
    17761 ggcccggtgc ccgatcttgc gtatcaggtc gattttccac ggctggaaat tgtgctggaa
    17821 ggtgagtttg ttgataccgg cgctggagca acgttagttc ccggcgatgt gctgtacgtt
    17881 gccgctggtg gctggaattt cccacaatgg aaaacaccca ctaccacctt tagcgtgctg
    17941 tttggcaaac agcaactcgg cttcagcgtc gtgcaatggg atggcaaaca atatcaaaat
    18001 ctggcgaagc aacacgtcgc ccgacgcggc ccacgtatag gttcttttct gctacaaacg
    18061 ctcaatgaaa tgcaaatgca gccgcaggag cagcaaacgg caaggcttat cgtagccagc
    18121 ctgcttagcc actgccgcga tttgctcggc agccaaatcc agaccgcctc acgcagccag
    18181 gcactatttg aagctattcg cgattatatc gacgaacgct atgcctccgc gcttacccgc
    18241 gaatctgtcg cacaggcatt ctatatttca ccaaactatc tctcgcacct gtttcaaaaa
    18301 acgggggcca ttggttttaa cgaatacctg aatcacacgc gactggagca cgctaagacg
    18361 ttattgaaag gctatgacct gaaggtaaaa gaagtggcgc acgcctgcgg ctttgtcgac
    18421 agcaactact tctgccgtct gtttcgtaaa aacaccgaac gctcgccgtc ggaatatcgc
    18481 cgacagtacc acagccaact gacagaaaaa ccgactactc cagaataaga tgggtttgct
    18541 gcggcgcaga aagcactttg cgcaccgcgc tcattacccg ctgcggctca cgcaggaatg
    18601 cgtagatgct gcattgcaca tagcggcaat gttcaaatcg ctcggcaccg gcaagctcga
    18661 tatccgtaat caacagagca acatccgccc gacggatatc ctcgtccgct aaacgattct
    18721 ccgttcccag cgcgccctga gtttcaatgc tgacgcccca cttctctaac tggcacaact
    18781 tttccagccg ttccgccgcc atataagtat gcgccacgcc gcttacgcag gcggttactg
    18841 ccaccaggta tgccatttta acctcccacg gtaacctgaa atccggcccg ttctgccatt
    18901 tcgcgcatcg ttgccacatc ggctgatgac ggcgcagcaa catccttcat cgaccatgtt
    18961 ttccccagca ggcggtattt cggctcgccg tactgatgaa acggcaacag atggatctgc
    19021 ctgatattca gcgggatcag cacgtccagc gcctgctgca tattctcccg gctgagtgtg
    19081 aaaccgggga tcagcggcaa acgcgggatc acgttgacac cctcactcac cagcaaacgc
    19141 aggttctcca gtacgcgtgg caggttcatc ttcaccacat cccgcgcctg agtcgcgtcc
    19201 ataattttta aatcgaacaa cacttcatcg cacaatttcg ccagcggtaa cagcttgctg
    19261 gctggtgcgt ctccggcagt ttcaatggca catgagacac cccacagccg cagtcgctgt
    19321 aaaaaacggg tagcaaactc cgcctgcatt aacacttcgc cgccagaaag cgtgacgccg
    19381 ccgccggacg tgcgaaaaaa aatgtcatct ttcatcactt cccgttccag agcgtcaagg
    19441 ctgatatcgc gaccaatccg ttcaaacgcc ccggaggggc attcatccgc atcacgcaaa
    19501 catttcgcac agtgcagaca tttcgcctct ctgcgtaccg tctggatttt gccggagatc
    19561 gactccggat tagcacacca cgggcaaaga tgcggacagc ctttaaaaaa gaccaccgta
    19621 cgaatgccct cgccgtcatt cagtgaataa cgctgaatgt tgaaaatgcg cgccacatca
    19681 ttgaggcgcg tttccacaac gttacagctg atgcgctgtc cggcggatga tgtcatcctg
    19741 gatctccttc gacagttcga caaagaaggc gctgtatccg gcaacgcgca ccaccagccc
    19801 ggcataatct tgtgggcgct gttgcgcttc ccacaacgtg tcggcgttca ccacgttaaa
    19861 ctgaatatgc tgcaacttaa gctgggtaaa cgcccgtaag aagtcggcca gtttgcgtaa
    19921 tcccgcttca ccttccaggg tcgccggagt gaatttcacg ttcagcaacg taccgttaga
    19981 cagcagcgta ttatcgagct tactgactga cttcagtacc gctgttggcc cttgttcgtc
    20041 ctggcccagc ataggtgaca agccgccgtc tgccagctgt tctccggcaa aacgaccgtc
    20101 tggcgtcgcg ccaaccaccg atcccaacgg gacgtgagca gaaacggtat acgatcccgg
    20161 cgtgaagtaa ccaccgcgcg ggttctggta tttttccact tctttgcagt agtggcgcaa
    20221 cagttcggcg ctaatgttat ccacctcgtc gatatcgtta ccatatttct caaagcggtt
    20281 aattaagcga gcgcggactt tttcgccttc tggcgttgca aagttggctt ttaataccga
    20341 cagcaattcg tcaaaactta aacgctgttg ctcaaaaacc atccctttca acgcatggag
    20401 agaatcgctc aggttggcga taccgatccc ctgtacgccg gagaagttat aacgcgcgcc
    20461 gccatcggta atatcgcgac ctttttccag acaatcgctg ataaaagacg agagcagcgg
    20521 tacaggtgcc cagtcgcgat ggccgatatc acaaatattg ctgccctcaa ccatcagggt
    20581 gatgtagtgg ctgatcttgg cgcggatctg ctccagtaaa ccttcataag tcagcgcagc
    20641 attgccttca ttttcatgca ggcagatttc catcactttc agcagattaa acatcgcgat
    20701 gtcatgcaag ccgtaggttc tgccgggaat agataattcc acacagccca ctacggaata
    20761 gtcgcgcgca tcttccagcg aaacgccacg gttgaggaac gctggtacca ccacttcatc
    20821 gttaaagatt tgcggaatac cggtgccgag gcgaatggtt tcggcggttt tcatcaggaa
    20881 tggcgtgtcg atcaaggcgt tagtgcgcac gccgaggttc ggttgcggta attgcacgct
    20941 ttgataggcg tcaaggcaaa ggaacgaaag cacgttcacc gcgctacgtc cgttctcggt
    21001 taacccgccg agcagtgcgg tatagccggt cgggaaacct gcgaaataac gcgcgctgct
    21061 ggtggagcgc aacagcacga tgtcgttgca tttcacccat aaagattcga gcagttcttt
    21121 caggaacgcc ggatcttcgc cctgggttaa agatgcctga tagaacggca acatatactg
    21181 gtcgaagcgc cctaaagaca gcgaactggc gttggattcg tattgcagaa tgatgttcat
    21241 gtaccagaat aactggcacg cctgccagaa cgtctgcggc ttatgttcgg cgttatggcg
    21301 agagatctcc gcaatagtca gcagttcttc gcgacgctgg gcatctgtgc agtttgccgc
    21361 catcgtttcc gccagtacag cgtaacgcaa aatgtgtttc tgcgaggctt ccagcagtaa
    21421 cagcgctgcc tgataaaagt gattctccgg ctgttgctga caatgttgct gcatctgtgc
    21481 caccaactcc cccagcccgt gattcaacag gcgtgggtaa tcaataataa tgtgcccctg
    21541 gcctttatcc gtctggttaa tgctgaaaat ctgcgtgctg gtcgcggctt ttacttcatc
    21601 cgtcatctgc ccgttgatga aatctttcat cgaacgtttt tcccagtacg ggaacaactc
    21661 ttcacgatag atacgtttat cttcttcgct gatagcaaag cggtcctgcg gacgcgtcgg
    21721 gaattgatcc agctctttca gcagccagta agggtccatt tccggcgaca taatcccggc
    21781 gcgcggtttt acggtgcggt taccggcaat cagttcttca tcacgaatcg aaatttcaac
    21841 atgttcaagg atatacgctg ttgctttcgc ccggcgcaat atcaccggtt cgccttcggt
    21901 ttgccgatgg ctggcggtat aaagcagcgc ccgctccagc gagatttcac gggtattggc
    21961 aaacagtgca gttttgaggc gagagatacg attcgtcatg aggaactcct gtgggaaagc
    22021 gaacccgccc ttgcgagcgg gagaagaact taagcggttt gcgccaggtg agcttcaatt
    22081 ttgctcataa tggcgtcagc acgttttact gcatcgctga tgttgacgcg cacgatggtc
    22141 ttgccagcaa aacgctcttc aaacttgatg ccgatatctt tggtcagaat caccatatca
    22201 gcgctcgcca catcttccgc agtcagttca ttttccagac caattgaccc ctgggtttct
    22261 actttcactt cccagccttt cgctttcgcg gcactttccc gcgcctctgc tgccatatag
    22321 gtatgggcaa cacctgaagg acatgcggtt actgcaataa ttttcgtcat ggctcgttcc
    22381 tcaattaatt aatttcaaaa tccaaatcca ggtcgtcttc tttttcatca gtcgaagacc
    22441 catttttacg cgccagactt ttcagcacgt tcacacaaac agccgtcacc actgctccga
    22501 cagccactgc tgcgatataa cccagcttgc cttcaaccac cggcagcaca atcagaccac
    22561 cccagcctgc atagcactgc gcaccgacca gcgccgcagt tactgcacca caaactgaac
    22621 cgaccatgat ggaaggaatg acacgcagcg gatcggcagc ggcgaaagga atcgcccctt
    22681 cagtaacgcc aacacagccc atcaccagtg ccgctttgcc ggtttcgcgc tcttctgcgg
    22741 agaaattttt acgaccaatc agcgtcgcca gccccatccc cagcggcggg atacaaatac
    22801 ccacggcagc gatgaccacc acggtataaa caccctgagc aacgcaaatc agcatgaagg
    22861 cataggctac tttgttaacc ggaccgccca tatcgaacgc cagcatcaga cccatgatca
    22921 ccgccagcat aacaatgctg ccctgctgca tcccctgaag ccactgagtc aggctgttgg
    22981 tcaacgcccc tacaggctcg cccaagcccc acatcatgat gcctgcggta atcagtgtgc
    23041 cgacgatagg aataatgaag ataggcatca ccgagcgcag aactttatgc accggaattt
    23101 tcttcaggta atgcaccacg atgccgccga taatcccggc gatcagtgca ccaaagaagc
    23161 ccgcaccaaa gctgttacca acccaggcac cgatagcgca cggagccagc gcagaacgct
    23221 ctgcaatgga gtaaccaatg taagcggcga ggaaaggcac catcagcgtc aaacctgcaa
    23281 cgccgatatc aaacagtttt ttcaaattcg gatcggctac ggcatccggc actgcgcctt
    23341 tgccatacaa catgacggaa accgccagca aaataccgcc cgataccacg aagggaatca
    23401 tgtgtgaaac gcccgtcatt aaatgctgac gggtattttt caggatctgc accaactcat
    23461 tcataagctg ctcccgggct tgcgaaagcc catgtccata tttttgatgg aggaaacaat
    23521 aggcaatcgc aggcggagca aacagtggat aaaaaggcca tttactggac ttttgtaata
    23581 tcagtacaaa aatgcgatcc gcctcataac ttgcgataaa ccggaagcat gccgcaaaac
    23641 ggcgtcttgt gagacgcgtc gcaaattgac ggttacatta catttgtcca gcttttggca
    23701 gatttgtcac atggcaggcc gttgccgtgc gctcttatcc tgtaaacaga aaaccgtacc
    23761 gggagagagg caatggccct gattgtggaa tttatttgtg agctacctaa cggcgtacat
    23821 gcgcgtccgg caagccacgt tgaaacgctg tgtaatactt tttcatcaca aattgagtgg
    23881 cataacctgc gcactgaccg caagggcaac gccaaaagcg cccttgcgct gattggcacc
    23941 gatacgctgg cgggcgataa ctgccagtta ctgatttccg gggccgacga acaggaagcg
    24001 caccagcgtt taagccaatg gctgcgcgat gaattccccc actgcgatgc gccgctggcg
    24061 gaagttaaat ctgacgaact ggaaccactg ccggtttcac tgaccaatct gaatccgcaa
    24121 attatccgcg cccgcaccgt gtgcagcggt agtgcaggcg gcattctgac gccgatctct
    24181 tctttagatc tcaatgcgct gagtaatctt cccgcagcca aagacgttga cgccgagcaa
    24241 tccgcactgg aaaatggcct gacgctggta ctgaaaaaca ttgagtttcg tctgctggat
    24301 agcgacggtg ctaccagcgc gatactggaa gctcaccgat ccctggctgg cgatacatct
    24361 ttacgtcagc acttactgac aggcgtcagc gagggattaa gctgtgccga agcgattgtc
    24421 gccagtgcga atcacttttg cgaagagttc gcccgttcca gcagcagcta cctgcaagaa
    24481 cgtgccctgg acgtacgcga cgtctgcttc cagttactcc agcaaatcta cggtgagcaa
    24541 cgcttcccgg caccgggcaa actgacgcag cccgccattt gtatggctga tgaactgact
    24601 cccagccagt tcctcgaact ggataaaaat cacctcaaag gtctgttact gaaaagtggc
    24661 ggcaccacct cacataccgt aattcttgcc cgttcgttca acattccgac actggttggt
    24721 gtagatattg atgcccttac tccgtggcag catcaaacga tttatatcga cggcaacgcc
    24781 ggggcgattg tggttgagcc aggggaagcc gtggcccgtt attatcagca agaagcccgc
    24841 gtacaggacg cccttcgtga gcaacagcgt gtctggctga cccaacaagc ccgtaccgct
    24901 gacggtatcc gcattgaaat tgccgctaac atcgctcact ccgtggaagc gcaggccgca
    24961 ttcggcaatg gtgcggaagg cgttggtttg ttccgcactg aaatgctcta tatggatcgc
    25021 accagtgcac cgggcgaaag cgagctgtac aacatttttt gtcaggcgct ggagtctgcc
    25081 aacggacgca gcattattgt gcgcactatg gacattggcg gcgacaaacc cgttgattat
    25141 ctgaacattc cggcagaagc taacccgttc ctcggttatc gcgctgtgcg tatttatgaa
    25201 gaatacgcct cgctgttcac cacacaacta cggtcgatcc tgcgcgcctc cgctcacggc
    25261 agcctgaaaa tcatgatccc gatgatctcc tcaatggaag agatcttatg ggtgaaagaa
    25321 aaactggcgg aagccaaaca gcaactgcgt aacgaacaca ttccgtttga tgagaagatc
    25381 cagcttggca ttatgctgga agtaccgtcg gtgatgttca tcatcgatca atgctgcgaa
    25441 gagattgatt tctttagtat tggtagtaat gacctgacgc aatatctgct ggcagtggat
    25501 cgcgataacg ctaaggttac tcgtcactac aacagcctga atccggcatt cttgcgggcg
    25561 ctcgattacg ccgtgcaggc ggtgcatcgc cagggcaaat ggattggtct gtgcggtgag
    25621 ctgggagcga aaggttccgt gctgccgttg ctggtcggct tagggctgga tgaactcagc
    25681 atgagcgcac catcaattcc ggcggcgaaa gcgcggatga cgcaacttga tagccgtgag
    25741 tgccgcaagt tgctcaacca ggcaatggcc tgccgtactt cgctggaagt ggaacacctg
    25801 ctggcgcaat tccgcatgac ccaacaggac gcaccgctgg tcaccgccga gtgcatcacg
    25861 ctggaaagtg actggcgcag caaagaagaa gtgctcaaag gcatgaccga taacctgctg
    25921 ctggcgggcc gctgccgcta tccgcgtaaa ctggaagccg acttatgggc gcgcgaggcc
    25981 gttttctcta ccggtctggg ctttagtttt gccattccac acagcaaatc agaacacatt
    26041 gagcaatcca ccatcagcgt ggcgcgtctg caagcgccgg tgcgctgggg cgatgatgaa
    26101 gcgcaattca tcattatgtt aaccctgaac aaacacgctg cgggcgatca gcacatgcgc
    26161 attttctctc gcctcgctcg ccgcatcatg cacgaagaat tccgtaacgc gctggttaac
    26221 gccgcctctg ccgacgctat cgccagcctg ctgcaacatg aactggaact gtaaaaggaa
    26281 acatcatgga actgtatctg gacaccgcta acgtcgcaga agtcgaacgt ctggcacgca
    26341 tattccccat tgccggggtg acaactaacc cgagcattat cgctgccagc aaggagtcca
    26401 tctgggaagt tctgccgcgt ctgcaaaaag cgattggtga tgagggcatt ctgtttgctc
    26461 agactatgag tcgtgacgcg cagggtatgg tggaagaagc gaagcgcctg cgcgacgcca
    26521 ttccgggcat tgtggtgaaa atcccggtga cttccgaagg tctggccgcc attaagatgc
    26581 tgaaaaaaga gggtattact acgcttggca ccgcagtgta cagtgctgca caagggttac
    26641 tggccgcgct ggcaggggca aaatacgttg ctccgtatgt taaccgcgta gatgcccagg
    26701 gcggggacgg cattcgtacg gttcaggagc tgcaagcgct gttagaaatg cacgcaccag
    26761 aaagcatggt gctggcggcc agctttaaaa cgccgcgtca ggcgctggac tgtttactgg
    26821 caggatgtga atccatcacc ctgcccttag atgtagcgca acaaatgctc aacacccctg
    26881 cggtagagtc agctatagag aagttcgaac acgactggaa tgccgcattt ggcactactc
    26941 atctctaaag gagcaattat ggaccgcatt attcaatcac cgggtaaata catccagggc
    27001 gctgatgtga ttaatcgtct gggcgaatac ctgaagccgc tggcagaacg ctggttagtg
    27061 gtgggtgaca aatttgtttt aggttttgct caatccactg tcgagaaaag ctttaaagat
    27121 gctggactgg tagtagaaat tgcgccgttt ggcggtgaat gttcgcaaaa tgagatcgat
    27181 cgtctgcgtg gcatcgcgga gactgcgcag tgtggcgcaa ttctcggtat cggtggcgga
    27241 aaaaccctcg atactgccaa agcactggca catttcatgg gtgttccggt agcgatcgca
    27301 ccgactatcg cctctaccga tgcaccgtgc agcgcattgt ctgttatcta caccgatgag
    27361 ggtgagtttg accgctatct actgttgcca aataacccga atatggtcat tgtcgacacc
    27421 aaaatcgtcg ctggcgctcc tgcacgtctg ttagcggcgg gtatcggcga tgcgctggca
    27481 acctggtttg aagcacgcgc ctgttcccgt agcggcgcta ccactatggc gggcggcaag
    27541 tgcacccagg cggcgctggc actggcagaa ctgtgctaca acactctgct ggaagaaggc
    27601 gaaaaagcga tgcttgccgc cgaacagcat gtagtaaccc ctgcgctgga acgcgtgatt
    27661 gaagcgaaca cctatttaag cggtgttggt tttgaaagcg gcggactggc ggcggctcat
    27721 gcggtgcata acggcctgac cgctatcccg gacgcgcatc actattatca cggtgaaaaa
    27781 gtggcattcg gtacgctgac gcagctggta ctggaaaacg cgccggttga ggaaatcgaa
    27841 accgtagctg catttagcca tgcggtaggt ttgccaataa ctctcgctca actggatatt
    27901 aaagaagatg tcccggcgaa aatgcgaatt gtggcagaag cggcatgtgc agaaggtgaa
    27961 accatccaca acatgcctgg cggcgcgacg ccagatcagg tttacgccac tctgctggta
    28021 gccgaccagt acggtcagcg tttcctgcaa gagtgggaat aacctactcc aaactcccgg
    28081 cttgtccggg agtttgaacg caaaattgcc tgatgcgcta cgcttatcag gcctacgcaa
    28141 tctctgcaat atattgaatt tgcgtgcttt ttaggccaga taaggcgttc acgccgcatc
    28201 cggcactctg tgccagtaat ctgaaactcc tacgttgttc aggagttttt tatccgcttc
    28261 tggacatttt ctctacagaa caatcgttag cccggaaggt cgaatctgtg actcttattt
    28321 tcacggacga accagatgaa agcttcactg gcgctgctca gcctgctaac ggcatttacc
    28381 agccactcct taaaatctcc cgccgttcca ccaaccgtgg tacaaatcca ggccaatacc
    28441 aaccttgcca tcgcagacgg tgccagacag caaatcggca acacgctatt ttacgatccg
    28501 gcgtatgtgc ggcttaccta tcctggcggt gatgtcccgc aagaacgcgg tgtatgttcc
    28561 gatgtggtga tccgtgcatt gcgcagccag aaggtcgatt tgcagaaact ggttcacgaa
    28621 gatatggcga agaattttgc cgagtacccg caaaaatgga agttaaagcg cccggacagc
    28681 aacatcgatc accgtcgggt gcctaatctg gaaacctggt ttagccgcca caacaaaaca
    28741 cgccccatca gtaaaaatgc cggtgactat caagcgggcg atattgtctc ctggcgatta
    28801 gataacgggc tggcgcatat tggggtggtg tcagatggct tcgcccgcga cggtacgcca
    28861 ctggtgatac ataacattgg cgcaggcgcg caggaagaag atgtgctgtt tagctggcgg
    28921 atggtggggc attatcgtta ttttgttaaa taagcgatcg ccccacatga agaaaaaatt
    28981 cagatgcttt ttacactgac agcttctttt ttcttacggc tcaccagcgc cagtgccagc
    29041 acaatcagca caataccgct accttcaacg gcccccggat tctcaccgag cagccaccag
    29101 gaaaagagta cgccgcaaac gggaacggcc agcgtgctta agctggcaat actggcaggc
    29161 aagtttttca atacaaacaa ccataagctc cacgccagcg ccgtcgccag aatcgcactg
    29221 taggccagcg cccagaacac ggtgggctgc cagtcaattt cacgttgcgg caccagtaaa
    29281 gcgaccacac tcatcaccag cgccgcatag agcatctgcc aggatgttaa cgataataaa
    29341 tccacgcgcg gatgacgggt gtataggcgt ttagcaacaa tcgcgctcgc cccccaacta
    29401 acaccggaga ggattgccag catggcactt ttcatcgaag agaaatcgag ttgccacggc
    29461 tgcaacacca aaaataagcc gaaagcggca atcagaatcg cgaaatattg cccacgtcgc
    29521 aggcgttcac cgagaaacaa cgcggcgaaa atcaccaccc agaacggcat ggtatagctc
    29581 aggatcgcca ccttccccgc acctccgctg accaacgccc actgcgccag accaaccatc
    29641 ccgcaggttt gtaacagggc aatggctaag gtgtatttaa acggcgtcgg gtgcattccg
    29701 cgaccacgta ataaaaggac gatgaataaa acgagagcgc cgaaaatgca gcgtaaggcg
    29761 gtaaagtcga aggcaccgat gtaactggtg acttgcttca tgaaaatcca gctataactc
    29821 cagataagtg tgagcacaac caggccactg attgccagtg ggttgctctt tcctgcggca
    29881 gacatgagag atccagtgtg tagttttcct gtggacacta tacgccggta aagaaacttc
    29941 agcgactgcc tgaaagacag tcactgaaga aggtcagatt acagcaggtc gaaacggtcg
    30001 aggttcatca ctttcaccca tgccgctacg aagtctttaa caaacttctc gtgggcatcg
    30061 ctgctggcgt aaacttccgc taccgcacgc aggacagagt tagaaccaaa taccagatcc
    30121 gcacggctgg ctgtgtattt cacttcgccg gtttcgcggt cacgtccttc gaacagctct
    30181 ttcgattcgt cagtcgcttt ccactcgtaa cgcatatcca gcaagttcac gaagaagtca
    30241 ttgctcaata cgccaacgcg gtcagtgaag acgccgtttt tgctgctatc gaagttggca
    30301 cccagtacac gcatcccgcc caccagcgca gtcatttccg gcgcggtcag cgtcagttgc
    30361 tgtgctttgt cgatcagcag tgactcggtg gtggaaacgt ccagacgagc gcgatagtta
    30421 cggaaaccgt cagcaattgg ctccagcagc tcaaacatct caatgtcagt ctgatcctga
    30481 cgcgcatcaa cgcgacccgg cgcaaacggt acatgaatgc tcaaacctgc ggcgcttgcg
    30541 gctttctcaa caccaaccac accagccagc acgatgatat ccgccagcga ggctttacca
    30601 gactctttct ggattttctc cagaacaggc agagcacgaa cggctgcggc gttcacatcc
    30661 cagtcgcgct gcggcattaa tgccagacgc gcaccgttgg caccaccgcg tttgtcgcca
    30721 ccacggaagg tagaagcaga tgcccaggcc accgatacca gctcactaac agacagacca
    30781 gaatccgcaa tcgcgaattt cagatcgata atgtcctgct cggtcgggtt gtagatcggc
    30841 tgcggcagcg gatcttgcca gatcagatct tctttcggca cttccggccc gatgtagcga
    30901 gatttcggcc ccatatccct gtgcgtcagt ttgaaccagg cacgggcaaa ggcttcgttg
    30961 aacgcctgcg gatcgttgag gaaacgacga gagatcttct cgaactcagg atcaaaacgc
    31021 agcgtcaggt cggtcaccag cattgtcggt ttacgtttct tcgacggatc gaacggatcc
    31081 gggataattt ccggtgcgtc taccgcttcg aactggattg ctccagccgg actgcgggtc
    31141 tgtacccact catatttgaa caggttctcg aagaaatagt tgctccactg ggtcggcgtc
    31201 tgggtccaga caacttccag accagaggta atggcatctg cgccaacgcc gctgccgtaa
    31261 gtgctcgccc agcctaaacc ttgttcttca atcggtgcag cttctggatc aggacctaca
    31321 tttgatgtcg gaccggcacc gtgagtttta cccagcgtat gaccacccgc aatcagcgcc
    31381 acggtttctt cgtcgttcat gcccatgttg ccgaaggtcg cacggatagc tgctgccgca
    31441 gaaagcggtt cgccgctgtg atccgggcct tccgggttaa cgtaaatcag acccatctcg
    31501 gttgcaccca gcggtgcttt cgccagcgct tccggatgac ggtgagtcag ccaggctttt
    31561 tcatcacccc agttaacatc cagatccggt tcccagacgt cttcacgacc ggcaccaaaa
    31621 ccgaaggtac ggaagccgga gttttccagc gccacgttac ctgcgaggat aaacaggtcg
    31681 gcccaggaga ttttctgacc atatttctgt ttgattggcc acaacaggcg acgcgcttta
    31741 tcaaggctta cgttatccgg ccaggagttc agcggtgcaa aacgttgctg accacgaccc
    31801 gcgccgccgc gtccatcgat tgaacggtaa gtacctgcgc cgtgccaggc catacgaata
    31861 aacagaccgg cgtagctgcc ccagtcggct ggccaccacg gctgagattc tgtcagcagg
    31921 gctttcagat cttttttcag gccgtagtaa tctaatttgc tgaattcttt gcggtagtca
    31981 aagtcctcac ccagcgggtt agaacgatta gaatgttggt ttaacaggtc aacacggagt
    32041 tgatttggcc accagtcgcg agtggttgtg cccgcccccg cgctctggtc gtgaccgccc
    32101 tgatggaacg ggcatttgcc agtggctgtc gtgttatgga tatcgtctga cgtgctcatc
    32161 aatgtgctcc cctctacagt gttaccgtta cgatacacag cgttagagag aagttataat
    32221 tgaattaatc cacggatgcg atagttgaga tcttacatat tgttggttaa agagatgtag
    32281 gtcaaattga tcgtaattaa gcttttttga ttatgtccga attcggacaa atgctttata
    32341 aaaaagggtt ttagcttcat atccttcaag taatgaggga gaaaacaagg ctgacaacct
    32401 tattttcccg ccctcatttc gaggcagcat tttgtgctct gtttaaaatt tgtgatcact
    32461 gtgtgatttt cacaaaagcc acactattta taaaccaggt cgaacaccca gcgtatggca
    32521 aatcgcgtaa ctcatttcag cgcggttaag cgtatagaag tggaaatctt tcactccttc
    32581 acggcttaaa atcttcacca tatccatggc aatattcgcg cccaccagtt tgcgggtttc
    32641 ggcatcatca tccaggccgt cgaacatttg cgccatccac gccggaatac gcacgttggt
    32701 catatcggca aatttcttcg cctgtttaaa gttagatacc ggcaaaattc ccggaataat
    32761 ttccacatca atgcccgccg atacacagcg gtcacgaaaa cgcaggtagc tttcgacatc
    32821 gaagaagaac tgagtaatcg cgcggttggc tccggcatcg actttgcgtt tcagattaag
    32881 caaatccgcc tgagcgcttt ttgcttccgg gtgaacttcc ggatacgccg ccacggagat
    32941 atcgaaatct gccacttctt ttaatagcgt caccaggtca gaagcataca tttctggctt
    33001 accacttccc ggcggcagat cgccacgcag cgccacgata tgacgaatac cgttattcca
    33061 gtagtcgcgt gcaatggtgc gcaactcgtc gggcgtcgca tcaatgcaag taagatgcgg
    33121 tgccgcttcc agaccagtgc gatctttaat gcctttaata atgctgtgcg tacggtcgcg
    33181 ctcgccggag ttcgcgccat aggtcaccga tacaaacttc ggtttcaggc tgctaaggcg
    33241 atcgatggag ttccacaggg tctgctccat ttcactggta cgcggcggga aaaactcgaa
    33301 cgaaacgtta atctgcccct ggacttctgc caggcgctga ttcagggcat cccgctggct
    33361 ggcgtgaaaa aagctcatac cttacctcat caatcgcttg tagttatatg ttgtgttgtg
    33421 cacatctatc cgtttagacg tccagatgta aagatgaagg aaaagccgaa gggcgtcaac
    33481 cgaaaaatga ccaatgatgg tgaggaaaaa tcagggaaga tgaaaaaact tcaggatggt
    33541 aagaaaaaga aaatccggta tgcaaagcaa accggactgc aaatataaac taaccatgta
    33601 cttattgctt cttgccggat tcgtcgtaaa cgcttaatcc ggcctaatcc tatgataatt
    33661 caatatattt cattagaaac cataaacccg aaaacatgag taccgggcat tattaaattt
    33721 ctgaaattac aacaactgtg ccagccggtt gatatccgac tgaatcgccc cggcggtgac
    33781 gtcgcgcccc gcgccaggcc cacggatcac cagcgggttg tcgcgatacc agcggctttc
    33841 gatggcaaag acgttatcgc acggcagcag tgatgccagc ggatggtctt cacgcaccgc
    33901 ctccacgccc acacgcgctt tgccgttggc atcgaaacgc gccacgtagc gcagcaccag
    33961 ccccatttcg cgggccgctt ccagccgttg caccatctgc tcgttaagtt catcgccatt
    34021 ttcaaagaaa tggtcgatgc tgccgccttc gcaatgagca ggcaccagcg attccacacg
    34081 tacctggtcc ggttcaatgt tgtaacccgc ttcacgcgcc agaattacca gcttgcgcat
    34141 tacgtctttg ccggagaggt cgtcacgcgg atcaggctcg gttaagccct gctgccacgc
    34201 ctgatccacc agctcggtaa acggcacgct accgtcgaat tgcaggaaca gccaggagag
    34261 cgtgccggag aagatcccgc tgatcgacaa aatagtatcg ccgctgtcga tcagatcgcg
    34321 cacggtgtgg ttgatcggca agcccgcacc gacggtggca ttgtacagcc agtgacgccc
    34381 ggttttttcg aaggcgtcgt ggatctggcg atatttattg ctgtcgctgg ctcccgccag
    34441 tttgttggcg ctgataacgt ggaaaccgtg gctggcgaaa tcaagatact gatcagcaag
    34501 ctgctggctg gcggtaacgt ccagcaccac taaatcatca tacggatggg cgcgcatcca
    34561 caggaacaac gactcttcat cctgctcaac cgcttcatcg ttgaagaagg ctaacgcgcg
    34621 gctggcgtcc agcccgtcat agctcaacag gctgcggcgg ctgtccacca cacctgccag
    34681 cacaaactca aagccggtac gtgccgaaag cgtgctctgc tcacgggcga acagttccag
    34741 ccaacgggaa ccgatattgc ccttaccgaa caataccagg ccgatgcgtt tttctgcgcg
    34801 gaagacggac tgatgcagcc cctgaatcag gctttcggtc gggccggtgc gcagtactgc
    34861 caccaggctg atgccgtcat cggactgcca ggtaaattcg accggctggc ctttcagttg
    34921 ctgccagaag cggtggcaat gcagcgggtt acgggtgacg cctgcaccga ccatcgccac
    34981 cagcgccagc ccctgacgca ggcgcagttc gccaggtaat cccgcttcgt cgaggatttt
    35041 cagcgcactg tcggccactt ctgaggtgta gcaaaattgc agcaactggc gatcgttatg
    35101 tacgccaacc gccagcgggc gtacctgcgc gcgtttcagg atttggtcga tctctttatg
    35161 cgccagtttg aaatcctgac tggcgggcac ctgaaactca atcaaacaga catcatcgtg
    35221 gctggtgaca atacgcgcac cagtaccgga ggccagcacg cgttcaatgc gcgtggaacc
    35281 ttgatccggc gtgtagctac agcgcagttg caggtcgatt tcgctgccag aaaccggctg
    35341 taaagtacgg gcgtgaagaa cgggagccgc caggcgcgcc agttcgctgg cctcatccag
    35401 acgcagcaac ggcagcaggc aggcatcttt cactttacgc gggtcggcac tgtatacccc
    35461 ggcgacgtcg ctccagatgg ttacgcgaga aacacccgcc agcgcaccga tttgtgtcgc
    35521 ggaatagtcg gaaccgttac gccccagcag caccgtttca ccggcgttgt tgcggctgat
    35581 aaatccggtc accaccagac gtttgcccgg atgttgcacc agcagctgtt gcagcaacgg
    35641 gtaagaaagc ccttcatcaa cctgcggttg tgcggcgcgt tcagcgcgta aaaactcgcg
    35701 ggcatcaagc caggccgctg gcagcccttg ttgattaagt accgcagaca tcaggcgtgc
    35761 cgaccatact tccccgtggc ccaccacttc cgcatacact gcgtcgttaa taccgctgtc
    35821 gagcagcgcc gccaggcgct caaggtcgct gacaaaagcg ctaatgaggc tatcagcttc
    35881 ttcagcgggt agcagaccgc taatcagatc gcactgatag cgacgcagcg tttgttgaac
    35941 ctgatgcgca gagagacgat cggtctggct tagtttcaac cagttaatca actggttagt
    36001 ggtgctaccg gcggcggaaa ccaccatcat atcgtcaggc tgagagtact ccgccataat
    36061 gcccgcaaca cgcaaataac acttcacatc agccagacta ctgccaccaa atttatgcag
    36121 ttgacgacct ttcgcccctg cctgcgcaat cacactcatt tttacccctt gtttgcagcc
    36181 cggaagccat tttccaggtc ggcaattaaa tcttcgccat cttcaatacc ggtggagata
    36241 cgcagcagcg tctcggagat cccggcggca gcacgcgctt ctggtgccat gcctgcatgt
    36301 gtcatggttg cggcgtgaga gattaaactt tccactcccc ctaatgattc cgccagcgta
    36361 aacaacgaca gcccgcccag gaaacgacgc agcgtctgct catcgccatc cagttcaaaa
    36421 ctcaacattg cgccaaagcc tttttgctgg cgcgcggcaa tttcatgccc ctgattttcc
    36481 ggcagcgacg ggtgatacag ttttttcacc aacggctggg tttgcagata tttcacaatc
    36541 gcctgcgcgt tgcgctgggc cagctccata cgcggcacca gcgttcgcaa cccgcgtagc
    36601 agcagatagc tgtcaaacgc gccgcccgtc acgccaatat tgtttgtcca ccaggcaagt
    36661 tcagtgacaa cgtccgggtc tttagcaatc accacgccag ccaccacgtc agagtgaccg
    36721 ttcagatatt tcgtacatga atgcaacacc agatcggcac ccaatgccag cggattttgt
    36781 aatgccgggc ttaagaaggt gttatccacc acgctcaccg ccccgacttc ccttgccaga
    36841 tggcagattt tcgcaatatc cacgacgcgt aacaatggat tacttgggct ttctaccagt
    36901 accagtttag gtttttctgc cagcgctgcc cgtaatgcct gttcatcgcc ttgatcaaca
    36961 aacaacacgc gatagcaacc gcgtttcgcc agactgtcga acaggcgata gctaccgccg
    37021 tagcagtcgt gcggcgcaac cagcagatcg ccaggtttca aaaagacggt cgttaccaag
    37081 tgaatcgcgg acatgccggt attagtaagt actgcgccag caccgccttc cagttctgcc
    37141 agcgcacgct gaaccacatc gcgcgttggg ttgccgcgac gcgagtaatc atgcgcgcgc
    37201 ggttcattaa atccggtaaa gttataggtg ctggaaagat ggatgggagg gacaacgcaa
    37261 ccatactgtt cgtcgtcatt taacccgcta cgcactgcga tggtggcctg tttacgcgtc
    37321 atgtgatgaa gtttcctggg ctttgtcggt gaaatgtcag gcaccagagt aaacattgtg
    37381 ttaatggacg tcaatacatc tggacatcta aacttcttta cgtatagatt gagcaaatcc
    37441 caaatagccg ttaaaattat atgcattatc acgccgacag gtgcattaca cgatgtcacg
    37501 gtaacgcctg tacggtaaac tatgcgggtt tacggtcagt acccacataa attgtgtggt
    37561 ctggtctcaa tttattgacg aagaggatta agtatctcat ggctgaatgg agcggcgaat
    37621 atatcagccc atacgctgag cacggcaaga agagtgaaca agtcaaaaag attacggttt
    37681 ccattcctct taaggtgtta aaaatcctca ccgatgaacg cacgcgtcgt caggtgaaca
    37741 acctgcgtca cgctaccaac agcgagctgc tgtgcgaagc gtttctgcat gcctttaccg
    37801 ggcaaccttt gccggatgat gccgatctgc gtaaagagcg cagcgacgaa atcccggaag
    37861 cggcaaaaga gatcatgcgt gagatgggga ttaacccgga gacgtgggaa tactaaagca
    37921 aaaaagagcg gcgcggagtg gaatcgcctg atgcgctacg cttatcaggc ctacgtcata
    37981 ttgcaattta ttgaatttgc acgaacttgt aggccggata aggcgttcac gccgcatccg
    38041 gcataaacaa cgagcacgtt gtctgcgacc caccgctttt tatacatgga cgtttaacta
    38101 tgaaaaacag gctgctgatc ctcagcctgc tggtttctgt acctgccttt gcctggcagc
    38161 cacaaaccgg cgacatcatc tttcagatct ctcgctcatc gcaaagtaaa gcgatccaac
    38221 tggcgaccca ttccgattat agccacaccg gtatgctggt gatacgcaac aaaaagccct
    38281 acgtttttga agcggtcggc ccggtgaaat acaccccgct caagcagtgg atcgcccatg
    38341 gtgaaaaggg caaatacgtt gttcgccgcg ttgaaggcgg actgagtgtt gaacaacagc
    38401 aaaaactggc gcaaacggta aaacgttatc tcggtaaacc gtatgatttc agcttctcat
    38461 ggagcgacga tcgccagtac tgttcggaag tggtgtggaa ggtttaccag aacgcgctgg
    38521 gaatgcgcgt gggcgagccg cagaagttga aagagttcga tctcagcaac ccgctggtcc
    38581 aggctaagct caaagaacgc tacggcaaaa atattccgct tgaggaaacg gtagtttcac
    38641 cacaagccgt tttcgacgcg ccacaactca ccacggttgc caaagagtgg ccgctgtttt
    38701 cgtggtaatg gatgatgaaa aaaataattc ccgccgtagt ctgtttgttg ctttgtagtt
    38761 gcacgacctt tccgcaaact gtcgctccgg taaacggtga tgcctccaat tagttgaatt
    38821 aaggatataa tactcgcttt taaggttgtt tcatggtcac agttaacctt cattgccccc
    38881 gttgccagtc cgttcaggtt taccgtcacg ggcagaaccc aaaaagacat gatcgattcc
    38941 gcggccgtga ctgtcaccgg gtatttcagc tcagttactg ttacgagccc cgtaaaccgg
    39001 gagttaaaga ccaaatcgct gaaatgactt tcaacggtgc cggggttcgc gatactgccc
    39061 gtacgctgaa aatcggcatt aatacagtca tccgaacttt aaaaaactct cgccacgaag
    39121 aataacgtca tccccggtcg ctcatgctga tgtggcactc atctgcgaac ttagtcagca
    39181 atggagcttt gtcggcagta aagcgcgaca gcactggctc tggtacgtgt acaacacgaa
    39241 aactggtggt gttctggcct acacttttgg cccccggact gacgaaacct gtcgtgaatt
    39301 gctggcgctg cttacccttt taccatcggc atgttgacca ctgacgaatg gggtagttac
    39361 accagagagt taccgaagga aaagcatctg actggtaaga tttttactca gcgcattgag
    39421 cgcaacaatc tgaccctgag aacccgaatc aaacgtctgg cccgcagaac catctgtttc
    39481 tcgcgctctg tagaacttca tgaaaaagtc atcggtgctt ttatcgaaaa tatatgttct
    39541 actaattgga gtcatcaccc ttctgatgag ctttccttgg atgattacca gactgaatac
    39601 acagccaaag atttttacct cattctgctc ttttttctca aatgacatat attatatttt
    39661 acatcat
//
LOCUS       DAZPEB010000014        36623 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZPEB010000014 DAZPEB010000000
VERSION     DAZPEB010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36623
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.014"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            92..988
                     /locus_tag="ACHURJ_000677"
     CDS             92..988
                     /locus_tag="ACHURJ_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1279744.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTNH"
     gene            complement(985..2463)
                     /gene="scpC"
                     /locus_tag="ACHURJ_000678"
     CDS             complement(985..2463)
                     /gene="scpC"
                     /locus_tag="ACHURJ_000678"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HFR1279745.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETDKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(2487..3272)
                     /gene="scpB"
                     /locus_tag="ACHURJ_000679"
     CDS             complement(2487..3272)
                     /gene="scpB"
                     /locus_tag="ACHURJ_000679"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFR1279746.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(3283..4278)
                     /gene="meaB"
                     /locus_tag="ACHURJ_000680"
     CDS             complement(3283..4278)
                     /gene="meaB"
                     /locus_tag="ACHURJ_000680"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFR1279747.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNTLRLGVTGTPSAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKIRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIEFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(4271..6415)
                     /gene="scpA"
                     /locus_tag="ACHURJ_000681"
                     /pseudo
     CDS             complement(4271..6415)
                     /gene="scpA"
                     /locus_tag="ACHURJ_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
     gene            complement(6619..7512)
                     /gene="argP"
                     /locus_tag="ACHURJ_000682"
     CDS             complement(6619..7512)
                     /gene="argP"
                     /locus_tag="ACHURJ_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFR1279748.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            7895..8554
                     /gene="rpiA"
                     /locus_tag="ACHURJ_000683"
     CDS             7895..8554
                     /gene="rpiA"
                     /locus_tag="ACHURJ_000683"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFR1279749.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            8809..10041
                     /gene="serA"
                     /locus_tag="ACHURJ_000684"
     CDS             8809..10041
                     /gene="serA"
                     /locus_tag="ACHURJ_000684"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFR1279750.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            10270..10329
                     /gene="ibsC"
                     /locus_tag="ACHURJ_000685"
     CDS             10270..10329
                     /gene="ibsC"
                     /locus_tag="ACHURJ_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFR1279751.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            10662..10721
                     /locus_tag="ACHURJ_000686"
     CDS             10662..10721
                     /locus_tag="ACHURJ_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR1279752.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(10822..11424)
                     /locus_tag="ACHURJ_000687"
     CDS             complement(10822..11424)
                     /locus_tag="ACHURJ_000687"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFR1279753.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(11445..11628)
                     /gene="ssrS"
                     /locus_tag="ACHURJ_000688"
     ncRNA           complement(11445..11628)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHURJ_000688"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(11670..11999)
                     /gene="zapA"
                     /locus_tag="ACHURJ_000689"
     CDS             complement(11670..11999)
                     /gene="zapA"
                     /locus_tag="ACHURJ_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFR1279754.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            12167..12745
                     /gene="ygfB"
                     /locus_tag="ACHURJ_000690"
     CDS             12167..12745
                     /gene="ygfB"
                     /locus_tag="ACHURJ_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFR1279755.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            12771..14096
                     /gene="pepP"
                     /locus_tag="ACHURJ_000691"
     CDS             12771..14096
                     /gene="pepP"
                     /locus_tag="ACHURJ_000691"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFR1279756.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIMNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     AEVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            14093..15271
                     /gene="ubiH"
                     /locus_tag="ACHURJ_000692"
                     /gene_synonym="visB"
     CDS             14093..15271
                     /gene="ubiH"
                     /locus_tag="ACHURJ_000692"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFR1279757.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            15294..16496
                     /gene="ubiI"
                     /locus_tag="ACHURJ_000693"
                     /gene_synonym="visC"
     CDS             15294..16496
                     /gene="ubiI"
                     /locus_tag="ACHURJ_000693"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFR1279758.1"
                     /translation="MQCVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADISLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(16570..16653)
                     /locus_tag="ACHURJ_000694"
     CDS             complement(16570..16653)
                     /locus_tag="ACHURJ_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1279759.1"
                     /translation="MSKNTKSKNNDIRKYNAKTEVKLVYFK"
     gene            16943..18037
                     /gene="gcvT"
                     /locus_tag="ACHURJ_000695"
     CDS             16943..18037
                     /gene="gcvT"
                     /locus_tag="ACHURJ_000695"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFR1279760.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            18061..18450
                     /gene="gcvH"
                     /locus_tag="ACHURJ_000696"
     CDS             18061..18450
                     /gene="gcvH"
                     /locus_tag="ACHURJ_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFR1279761.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            18569..21442
                     /gene="gcvP"
                     /locus_tag="ACHURJ_000697"
     CDS             18569..21442
                     /gene="gcvP"
                     /locus_tag="ACHURJ_000697"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFR1279762.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETSISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSHEVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            21708..22451
                     /locus_tag="ACHURJ_000698"
                     /pseudo
     CDS             21708..22451
                     /locus_tag="ACHURJ_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(22508..23941)
                     /gene="bglA"
                     /locus_tag="ACHURJ_000699"
     CDS             complement(22508..23941)
                     /gene="bglA"
                     /locus_tag="ACHURJ_000699"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFR1279763.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEIPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMINAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWCWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            23986..24297
                     /gene="yqfB"
                     /locus_tag="ACHURJ_000700"
     CDS             23986..24297
                     /gene="yqfB"
                     /locus_tag="ACHURJ_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFR1279764.1"
                     /translation="MQPNDITFSQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            24461..25120
                     /locus_tag="ACHURJ_000701"
     CDS             24461..25120
                     /locus_tag="ACHURJ_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFR1279765.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSMCHFLAIYLYIGQ
                     A"
     gene            complement(25316..26296)
                     /gene="ygfZ"
                     /locus_tag="ACHURJ_000702"
     CDS             complement(25316..26296)
                     /gene="ygfZ"
                     /locus_tag="ACHURJ_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFR1279766.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMTENQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAADDERVLLGVAGFQARAALANLFSELPSREKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            26539..26805
                     /gene="sdhE"
                     /locus_tag="ACHURJ_000703"
     CDS             26539..26805
                     /gene="sdhE"
                     /locus_tag="ACHURJ_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFR1279767.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            26786..27193
                     /locus_tag="ACHURJ_000704"
     CDS             26786..27193
                     /locus_tag="ACHURJ_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFR1279768.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(27233..27754)
                     /gene="fldB"
                     /locus_tag="ACHURJ_000705"
     CDS             complement(27233..27754)
                     /gene="fldB"
                     /locus_tag="ACHURJ_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFR1279769.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            27866..28762
                     /gene="xerD"
                     /locus_tag="ACHURJ_000706"
     CDS             27866..28762
                     /gene="xerD"
                     /locus_tag="ACHURJ_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFR1279770.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            28787..29497
                     /gene="dsbC"
                     /locus_tag="ACHURJ_000707"
     CDS             28787..29497
                     /gene="dsbC"
                     /locus_tag="ACHURJ_000707"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000715205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFR1279771.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGAAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLESQAEQQMKAIW
                     CAKDKNKAFDDVMAGKAATPASCDIDIADHYALGVQLGVSGTPAIVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGN"
     gene            29503..31236
                     /gene="recJ"
                     /locus_tag="ACHURJ_000708"
     CDS             29503..31236
                     /gene="recJ"
                     /locus_tag="ACHURJ_000708"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFR1279772.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQIEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            31327..32425
                     /gene="prfB"
                     /locus_tag="ACHURJ_000709"
     CDS             join(31327..31401,31403..32425)
                     /gene="prfB"
                     /locus_tag="ACHURJ_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFR1279773.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIDINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            32435..33952
                     /gene="lysS"
                     /locus_tag="ACHURJ_000710"
     CDS             32435..33952
                     /gene="lysS"
                     /locus_tag="ACHURJ_000710"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFR1279774.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITK
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(33995..34543)
                     /gene="idi"
                     /locus_tag="ACHURJ_000711"
     CDS             complement(33995..34543)
                     /gene="idi"
                     /locus_tag="ACHURJ_000711"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFR1279775.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTNSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            34598..34669
                     /locus_tag="ACHURJ_000712"
     CDS             34598..34669
                     /locus_tag="ACHURJ_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1279776.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(34666..34791)
                     /gene="yqfG"
                     /locus_tag="ACHURJ_000713"
     CDS             complement(34666..34791)
                     /gene="yqfG"
                     /locus_tag="ACHURJ_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFR1279777.1"
                     /translation="MNLLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(34793..36241)
                     /gene="uacT"
                     /locus_tag="ACHURJ_000714"
     CDS             complement(34793..36241)
                     /gene="uacT"
                     /locus_tag="ACHURJ_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFR1279778.1"
                     /translation="MSAIDSQLLSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSVGL
                     SIIQVGIDWAAGGKGNPQYGNPVYSGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDALWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
BASE COUNT         8822 a         9223 c         9796 g         8782 t
ORIGIN      
        1 cccccggatc atggacgact gggggcagta atcatgccgg gtctggctgg ctaacacccc
       61 attataagga gtacgaataa agtaacgggg gatggacatt tttatttcga aaaagatgcg
      121 caatttcatt ttattagcgc aaaccaataa tattgcccgg gcggcagaaa aaatccatat
      181 gacggcttcg ccatttggta aaagcattgc cgcgctggaa gaacaaattg gctatacgct
      241 atttacccgc aaagataata atatcagcct caataaagcc ggtcaggaac tgtatcaaaa
      301 actgttcccg gtttatcaac gactttctgc gattgataat gaaatccata actcagggcg
      361 tcgttcacga gaaattgtga ttggcataga taatacttat ccgacaatta ttttcgatca
      421 gttgattagt ctcggagata agtatgaagg cgtgaccgcc cagccagttg agttcagcga
      481 aaacggcgtt atcgataatc tttttgaccg tcagttagat tttattatct ccccacagca
      541 tgtatctgca cgagttcagg aactggaaaa cctgactatc agtgaattac caccgctgcg
      601 attgggcttt ctcgtttctc gccgttatga agagcgtcaa gagcaagagc tgcttcagga
      661 gctgccctgg ctacaaatgc gctttcaaaa ccgggctaac ttcgaagcaa tgattgatgc
      721 gaatatgcga ccttgtggga tcaatcccac cattatttat cgtccctaca gttttatggc
      781 caaaatcagc gccgtggagc gcggacattt tctgacggtg atcccgcatt ttgcctggcg
      841 actggtgaat ccggcaacgc tgaaatattt tgatgcgccg cacaggccga tgtatatgca
      901 ggaatatctt tattcaatca gaaatcatcg ttataccgcc acgatgcttc agcatattgc
      961 tgaagatcgt gacgggacaa accattaacc cagcatcgag ccggttgcaa ttaaattacg
     1021 gtgtaagtcg aagacgtggc taagatcgtg gtgaatatgt ccgccgggcg cattttccag
     1081 atagcgatgc agataatccc gatacatagg atgtgcacaa ttatcaatga tagtgcgggc
     1141 gcgttgaagc ggggaaagac cgcgcagatc ggcgatccct tgttcggtga tgatcacttt
     1201 gacgctgtgt tcgctgtgat caacatggct gcacattggc acgacggttg agatcttgcc
     1261 ttctttagca atcgacggag ccataaagat cgacagatac gcgttgcgtt caaaatcacc
     1321 gctgccgccg atgccgttca tcagatcgac cccggctacg tgcgttgaat ttgcatgccc
     1381 gtaaatatca aactccaggc cgacgttcag agcgatgacg cccagacgac ggatgatttc
     1441 cgggttattg gaaatctcct gcggacgcaa cacaatgcgg ctggcaaagt aatccatatt
     1501 gtcgtaaatc ttgcgcaggg aatcggccga gattgtcagg ctggaggcgc tggccccgct
     1561 gattttgtcg gtttccagta aatgcaccac cgattcctgt agcacttccg aatacatcat
     1621 gaacggagga atttccgggt tttcccccag acgcgccatt accgcattat tgatattgcc
     1681 cacgccactt tgcagcggca gaaattccgg cggaatacgc ccatgcgcca tttcctgcaa
     1741 taagaacgtg accacgttat cggcaatctg ctggcacatg ggattttgct tatccagcat
     1801 attaccggcg tcgggcaagt tggtttccac gacggcgaca atctttttcg gatcgatttg
     1861 cacatagcgg gtaccgacgc gatccattgc atggaagatc gacacgctat tgcgccgcgg
     1921 tggcgcgcca ggaatcacaa tatctgccag ttctgcaacg cgcggatcgt gatagtgatt
     1981 aagttcaatg atcactttct tcgctcgcag cagccaggtc ggcgcattac cgatcccgct
     2041 ggttaaccag actcgaccat ccggtgccag tgccgatgct tcaatgacgg caacatcaat
     2101 gtcgccgaag aaaccgtaat tgaccatttg cgccacttcg ctcaaatgca ggtcaacgaa
     2161 actcaccgcg ccctgattga tctttttacg taaaccggac gatgtttgat atggcgcacg
     2221 ccaggaaaca gcatcggcgt cagaaagtac atcgtcagcg gcggcgctga ttgacgcccc
     2281 cgtcagcagg cgaatttgat acggcttttt ggcctcatgc tgttcgttag ctctgcgggc
     2341 aatcgcggtg ggtagggctt tcggcgaacc cgccggggta aagccgctaa atgccaccat
     2401 gtcgttatgc tggataattt ctgccgcttc atcggcggtc atccttgtcc actgagtttc
     2461 catttttact ccttcgttcg cagggattaa tgaccaacga aattaggttt acgtttttcg
     2521 aggaaagcgt tcattccttc ctggtaatct tcgctgtcat aaaccgcgcg tcgcatcccc
     2581 tgaatacgtt caaattcatc ggagttcatg gtgtgcgctt cgcccagtac acgcagctct
     2641 tctttgataa cagcaatggc taacggcgct ttctcagaga tgtggtgcgc catttgtaag
     2701 gtgaaatctt ccagttcttc cacttccaca acatggttga ggatgccgac agccagcgcg
     2761 cgctgggcgg tgattggcga agcggtaaaa atcagctctt tgacaatgtg gaagcccgcg
     2821 tcgcgggtca ggttgtgaat gccgaccagg ttatacggga cgccgaggtt tacaggcgtc
     2881 attgagaagg ttgaggtact ggcggcgatg atcagatcgg aactcatgat catttcaaat
     2941 gcgccacccc aaacactacc ttccaccatc gaaatgatcg gtttcgggaa tttttggatc
     3001 atgcgggtga tttgacgcaa tggatcatca taggagagcg gatcgcgacc gccagacggc
     3061 agttcgtgaa tatcgtgacc tgcggagaag actttggatc cactcggcgc gcgcaaaatg
     3121 atacagcgaa tttccggccg gttgagatcg cttaacgcct gcataagatc atcaataaag
     3181 actttactta aggcattaag ttttcggcca tagttaaact caatgaccgc cactttgttg
     3241 atagtggcaa cgttcacata ctgataagac ataaaaattc ctttaatcaa aatattgcgt
     3301 ctggataaat tcactgagct gccgcaggcc ggtgcgcggt gagagcgtat tgtttttgac
     3361 cgctaaaagc gtctggcggt aatagcgatc gaaatcttca ttcgcgaaca ggtgattcag
     3421 tacttcttct tcggtctgct tacgcagcca ttccaccgat tgttgttgcc gcacttgttg
     3481 taaacgacca ctggcagtta gcgcggtttt gaactcgatg atggcgtgcc agatctcatc
     3541 gattccacgt ttttccagtg cgctacaagt cagaacccgt ggctgccatt cgtcgtattt
     3601 acgtcgcaga atatgcaggg cactctcgta catatgccgg gcaatggcga cattggtatg
     3661 gttatcgcca tcgtctttgt tgataacgat cagatcagcc acttccatca gccctttttt
     3721 aatgccctgc agatcatcgc cgccaccggc aatttgcaac gagataaaac agtccaccat
     3781 gcgggcgact tctgtttccg actgcccgac gccaaccgtt tcgacaatca ctacgtcata
     3841 acccgccgct tcgcataaca gcattaattc ccgcgctcgc tgactggcac cgcccagatg
     3901 accggaggat ggtaccgggc gaataaacgc cgcttcggca cgcgccaggt cattcatgcg
     3961 gattttatcc ccgagaatgc taccgccagt gaccgggctg ctgggatcga ccgcaataac
     4021 cgcgaccttt aatccctctc gaatcaacaa catgccaaag gcctcaagaa aggtactttt
     4081 ccccgcgctg ggggtgccgg taacgcccag tcgcagggtg ttaccgcagt acggcataat
     4141 ggcatcaagc agctgcgtac ttagtgcctg atgacgcggg tgacggcttt ccaccagcgt
     4201 catggcctgg gcgagtgtgg cacgctcacc ctgacgtaag cggcgaatac tttctgccag
     4261 cgtggcttca ttaatcatga tgctggctta tcagattcag tacgtcgcgc acactgtcga
     4321 gcataggtgt acctggacca taaatcgccg ccacgccgcg ctcttgcagg aaggcgtaat
     4381 cctgcggtgg aatgacgcca ccggcgacta cgcagatatc ttcgcgtccc cattttttca
     4441 gcgcctcgac cagttccggg atcagcgttt tatgaccggc agccagtgag gatgcgccca
     4501 ctacgtgaac gtcattttct actgccaggc gggcgatctc ttcaggtgta gagaacatcg
     4561 ggcttaaatc tacgtcgaaa ccgagatcgg aataggcgct ggcgatcact ttcgagccgc
     4621 gatcgtgccc atcctggccc attttggcga tcagaatgcg caggcgacga ccattgtcgg
     4681 caaggaactg ctccgtttgc gcaacaatgg catcgaactc ggaggccgat ttctcagatt
     4741 gatgatagct ttgcgcaatc acgccggtaa cacactggct tggcaccaga taacggtcga
     4801 aagcggcttc cagcgcatcg gaaatttcac ccagggtggc gcgaacgcga gcggcattaa
     4861 cagcggcagc cagcaggttt tcgttatgct gtgcggcgtg agtcagggcg ttcaacgcgg
     4921 cggttacggc ggcatcatca cgggtggcgc gaatgcgttc cagcgaagca atttgctcgt
     4981 tacgcaccat cacgttgtcg atctcaagta catcggtttc gtcttcgtga tccagtttgt
     5041 acttgttgac accaacgatg acacgtttgc cctggtcgat aagcgactgt tcgcgcgctg
     5101 aggcctattc gatcattcgt tttggcagac ctgcttcgat cgctttcgcc ataccaccgg
     5161 cttcgtcgat ctgttggata atagctctgg cttgtttgac gatttgatcg gtcagcgact
     5221 caatgtaata ggatccggcc agtggatcga cggtgcggca gagttctgat tcttcctgga
     5281 tgatgatctg ggtgttgcgg gcaatgcgtg ctgagaaatc ggtaggcaaa ccaagcgctt
     5341 cgtcaaaggc gttggtatgc agtgactgag taccgcccag cgtcgcagcc agtgcttcaa
     5401 tggtggtgcg gataacgttg ttatacggat cctgttcagt caggctccag cctgaggtct
     5461 ggcagtgggt acgcagcgcc agtgatttcg ggtcctgtgc gccaaatcca ctgaccgctt
     5521 cgctccatca ataacgtgcc gcacgcaaca tggcgacgtt cataaacaga tccatgccga
     5581 tgccgaagaa gaacgacagg cgaggagcga agtcatcaat tttcagtccg gcagagattg
     5641 ctgctttgat gtactcaatc ccatcagcga gcgtaaatgc tacctgctgc acgcagttgg
     5701 cacccgcttc acccatgtgg taaccgctga tactgatggt attaaatcgc ggcatgttgc
     5761 cggaacacca ggcgatgatg tcggcgataa tgcgcattga cggttttggc gggtaaatat
     5821 aggtgttgcg gcagaggtac tctttgagga tatcgttctg aatggtgccg gtcagtttat
     5881 caggtgtaac accttgctct tccgcggcga cgatataaaa cgccagtact ggtagcactg
     5941 cgccattcat ggtcatcgaa accgacattt tatccagcgg gatctggtcg aacaggactt
     6001 tcatatcttc cacggtgtcg atagcgacgc ccgctttgcc gacgtcgccc gccacgcgcg
     6061 ggttatcgga gtcgtagcct cggtgggtgg caaggtcaaa cgcaacggaa agaccttttt
     6121 gcccggcggc caggttacgg cgataaaaag cgttggactc ttttgctgtt gaaaaaccag
     6181 catactgacg gatggtccac ggttgggcgg tatacatagt ggcacgcggg ccacgaacgt
     6241 agggcggcaa accaggaagg gtacctgtca cctccagatt atcgagatcg gcttcggtat
     6301 acagcggctt gatggtgatc ccttccgcgg tttgctgaac cagcgagtcg acagttttct
     6361 cccgacggct caattccttg ttggcaagct gttgccactc ctgcacgtta gacattattt
     6421 gctccgtaaa acgcaatcaa aatgagggta tttgcgccta cggtgagctt tttacgtggg
     6481 tttgtcgcca gcaaaaaaaa tgcattcaga cgtcgttttt tgccgcctga gatttgtcta
     6541 ttttgtgatg tgattcaagg aatacaaaaa gaagtgagga caacgcctga tatgtgctat
     6601 caggcattat ttgatggatt aatcctgacg aaggactttg tgaccataat cgagcaacgc
     6661 atcagtgact ttacgcatca tgcggctttc aggagcaaag cggtgccagt agagcatccg
     6721 gcgttgaaat agccctggcg ttaagtcaat cagttcaccg ctggctagtt ctttctcgat
     6781 ttgcaggtgc gggatcatac agcaggtggt gccctggcga gcaagttgca cgaacgcttc
     6841 tgaagaatta acgatatggc agggcacgct gcctggaggc agatcgaagt tttgctgcaa
     6901 aaaggcctgg tgcatatcgt caagatggtc aaaggcgacc acaggtgctt tcaataatgc
     6961 cgaacgcgtt acgccgttag ggaaatattt ttcggcaaac ggttttgagc tgacgaacag
     7021 atagtcaagc gcaccgagct tatcgacaag gcaactcggc agcgcctgat gttgaatact
     7081 caccgcgccg accacttcgc cgcggcgcag acgttcctga gtacgggttt catcttctac
     7141 ctgcaagttg aggcggatag gcgaatcagc caatacagga gccagtgccg gaagcaacca
     7201 cgtcgccaga ctgtcggcgt tgaccgccag tgaaagcagc agcggagtcg aaccggtttg
     7261 ttcatcgccc agccactctt cttccagcaa ctccacctgg cgcagcagtg ccagcagttt
     7321 ttgcccttgt tccgtcgggc gcggcggtac ggtacgcacc aacagcggct gcccgaacat
     7381 attttccagt tgcttaatgc gctgtgagac ggctgattgt gtaatgcaca gcttttgtgc
     7441 cgcgcgctca aatcctcgtt cacgtatcac cgcatccagt gcctgtaatg ttctgtagtc
     7501 cgggcgtttc attgctgttg tctgctcctg gtaaatgctt tttcctgcac tatgacacaa
     7561 ttttgtgtca ggttgcatat accgcagccg tcggcgggta acatttgtat caatggttaa
     7621 acgcgcagct taaaagatca gggatttgcg ctattgcatt tcgcttagcg acttcgcgcc
     7681 ctggttatgt tgaatggcgt ggcgttattg cctctgttcg caccgtggag cggcataccc
     7741 agaccgttgt atgctttatt tgcggggcga aaggggatgc ccgccattgc gcgatatttt
     7801 atggatgagt taaccacccg gcttgccaac ggggtctgaa tcgctttttt tgtatataat
     7861 gcgtgtgaaa tttcatacca caggcgaaac gatcatgacg caggatgaat tgaaaaaagc
     7921 agtaggatgg gcggcacttc agtatgttca gcccggcacc attgttggtg taggtacagg
     7981 ttccaccgcc gcacacttta ttgacgcgct cggcacgatg aaaggccaga ttgaaggggc
     8041 cgtttccagt tcagatgctt ccactgaaaa actgaaaagc ctcggcattc acgtttttga
     8101 tctcaacgaa gtcgacagcc ttggcatcta cgttgatggc gcagatgaaa tcaacggcca
     8161 catgcaaatg atcaaaggcg gcggcgcggc gctgacccgt gaaaaaatca ttgcttcggt
     8221 tgcggaaaaa tttatctgta ttgccgacgc ctccaagcag gttgatattc tgggtaaatt
     8281 cccgctgcca gtagaagtta tcccgatggc acgtagtgca gtggcgcgcc agctggtgaa
     8341 actgggcggt cgtccggaat accgtcaggg cgtggtgacc gataatggca acgtgatcct
     8401 cgacgtccac ggcatggaaa tccttgaccc gatagcgatg gaaaacgcca taaatgcgat
     8461 tcctggcgtg gtgactgttg gcttgtttgc taaccgtggc gcggacgttg cgctgattgg
     8521 cacacctgac ggtgtcaaaa ccattgtgaa atgatctgac gggggaacct cccccgttaa
     8581 aaaaattctc ttcattaaat ttggtgacat gtgtcacgtt tttacctggc tattgtcgat
     8641 tgctctaaat aaatcctcta aaccagcata ttcaaccaag aaataccttt gcgtgatatt
     8701 tcctcaacat cgcgacgcaa acgttcatat tgccgcaata ttattttttg atatgttgaa
     8761 aggcggatgc aaatccgcac acaacatttc aaagacagga ttgggtaaat ggcaaaggta
     8821 tcgctggaga aagacaagat taagtttctg ctggtagaag gcgtgcacca aaaggcgctg
     8881 gaaagccttc gtgcagctgg ttacaccaac atcgaatttc acaaaggcgc gctggatgat
     8941 gaacaattaa aagaatccat ccgcgatgcc cacttcatcg gcctgcgatc ccgtacccat
     9001 ctgactgaag acgtgatcaa cgccgcagaa aagctggtcg ctattggctg tttctgtata
     9061 ggaacaaacc aggttgatct ggatgcggcg gcaaagcgcg ggatcccggt atttaacgca
     9121 ccgttctcaa atacgcgctc tgttgcggag ctggtgattg gcgaactgct gctgctattg
     9181 cgcggcgtgc cggaagccaa tgctaaagcg caccgtggcg tgtggaacaa actggcggcg
     9241 ggttcttttg aagcgcgcgg caaaaagctg ggtatcatcg gctacggtca tattggtacg
     9301 caattgggca ttctggctga atcgctggga atgtatgttt acttttatga tattgaaaat
     9361 aaactgccgc tgggcaacgc cactcaggta cagcatcttt ctgacctgct gaatatgagc
     9421 gatgtggtga gtctgcatgt accagagaat ccgtccacca aaaatatgat gggcgcgaaa
     9481 gaaatttcac taatgaagcc cggctcgctg ctgattaatg cttcgcgcgg tactgtggtg
     9541 gatattccgg cgctgtgtga tgcgctggcg agcaaacatc tggcgggggc ggcaatcgac
     9601 gtattcccga cggaaccggc gaccaatagc gatccattta cctctccact gtgtgaattc
     9661 gacaacgtac ttctgacgcc acacattggc ggttcgactc aggaagcgca ggagaatatc
     9721 ggcctggaag ttgcgggtaa attgatcaag tattctgaca atggctcaac gctctctgcg
     9781 gtgaacttcc cggaagtctc gctgccactg catggtggtc gtcgtctgat gcacatccac
     9841 gaaaaccgtc cgggcgtgct aactgcgctg aacaaaatct tcgccgagca gggcgtcaac
     9901 atcgccgcgc aatatctaca aacttccgcc cagatgggtt atgtagttat tgatattgaa
     9961 gctgacgaag acgttgccga aaaagcgctg caggcaatga aagctattcc gggtaccatt
    10021 cgcgcccgtc tgctgtacta attccccttc tctgaaaatt aacgggcagg tcactgactt
    10081 gcccgttttt ttatcccttc tccacaccgt tgccgcgttg cgcattctac tttgcgagtc
    10141 tgctccagaa aataaatcaa agtcagatca atgcgttgtg tttatttaat ggggatttac
    10201 gatggcggga cagtatgggg ctgtaacggt aaagccctca ccgaagcgag ggcttaaagg
    10261 agaagggtta tgatgcgact tgtcatcata ctgattgtac tgttactgat aagtttcgga
    10321 gcttattaac agtcagtctc tggggaggag tcatcctccc ttacccttac tcactaaatt
    10381 aggtcaaaga atcaacgatg tcaatcaggg cgatgcgggg tgcatcgccc tgaaccgaac
    10441 tttttgatag ccacttcatc gcccacgttt actgccgtaa tagatccttt ttcccaaaaa
    10501 ttatctctgt tttgcatggg gtttttcgaa cacgctcgaa gaaaagattt aaaggtagat
    10561 caatgcgttg tggttattta aagagagctt acgatagctg ggcagcatgg ggctgtaacg
    10621 gtaaagccct caccgaagcg agggcttgaa ggagaagggt tatgatgcga cttgccatca
    10681 tactgattgt actgttactc ataagtttcc cagcttatta acagtcagtc acaggggagg
    10741 agcaatcctc ccttaccctt actcactaaa ttaggttaaa gaatcaacga tgtcaatcag
    10801 ggcgatgcag gtgtatcgcc cttaccactc ccagactttc gacggtgtaa ccaccgcagg
    10861 aagagggata tcccactctt caacggggag tttttccacc aactgacaat catgcgcata
    10921 acccaccggt tgcgttttat agtgctgcca gttttgtaag gtccgatcat aaaaaccacc
    10981 gcccattccc aggcgctgac cgtactcatc aaaggcgacc agcggtgtga tcagcacgtc
    11041 taatcgggaa aggggtagca cgtcacgcac atccagtttt ggctcatgga ttttcaacct
    11101 gttcatcacc agttcgcttt gcggatggta attcaggaac agcaaattac cagcactaaa
    11161 gggatgcaaa actggaagat atacgcgctt accggcgcgc cagagttgtt ctattagcgg
    11221 ctgggtgtcg agttcaccat caaaagagag gaatacagcg accgtatgcg ccatcaccac
    11281 cgggggataa gtcatcatcc gggtagcggc ttgttgaccc atttcctgct gttgttccgg
    11341 cgttaacgca cgacgacgtt gccgaatcat tttgcggatt tcttgtcggg ataatgtcag
    11401 tgggagttct ggtagttgcg tcatggctgg tgccagataa gaagggaatc tccgagatgc
    11461 cgccgcaggc tgtaaccctt gaacccttgg ttcaaggtga atgtgtcgtc gcagttttaa
    11521 ggcttctcgg acggaccgag catgctcacc aaccgcggag cgccacattc ttgtggtatg
    11581 aaatatcggc tcaggggact ggcccgcttg cgaacatctc agagaaattt tgtcttcacg
    11641 gttactctac cacagtaaac cgaaaagtgt cattcaaagt tttggttagt tttttcggtg
    11701 atgcgacctt gttcaagtaa cgcttgttct atggtctgct gcagcatccg aatacgctgt
    11761 tccatacttg ccgcgtagtc acgagtcttc gctttttctt gcgctaactc atagctgata
    11821 ttcaatgcgg caatgaagac caactgttca gtatttgtga ctctagtgcg ttctttcaga
    11881 tcttgcaacc gttggttcag atcgtccgct gcctgattca acgcatccct ttggtcaggc
    11941 gggcagttca cacgcagtga acggccaaaa atttggatat cgacgggttg tgcagacatg
    12001 ccaccttcct gctgattgac tgcgctgcct tcgtctttag acccatggtc tgcgaagggg
    12061 cgacactata gctaccctga tgagaagaga caagcccttt tctggtccac cagggcccaa
    12121 agtggtagca tatcatgaat attcctccct ttgacgacga atgcttatgt ctatacagaa
    12181 cgaaatgcct ggttacaacg aaatgaacca gtatctgaac caacaaggga cgggtctgac
    12241 ccctgccgag atgcatggtt taatcagtgg gatgatatgt ggcggtaacg atgacagctc
    12301 atggctgccg ctacttcacg acctgacgaa cgaaggcatg gctttcggtc atgagctggc
    12361 acaggcactg cgtaaaatgc actctgccac cagcgatgcc ctgcaggatg acggcttcct
    12421 ttttcagctt tatctgcctg atggcgatga tgtcagcgtt ttcgatcggg ctgatgcatt
    12481 ggcaggttgg gtcaatcact tcctgcttgg tcttggcgtt acgcaaccga agctggataa
    12541 agtgaccggc gaaaccggtg aagctatcga cgatctgcgt aacattgcgc aactgggtta
    12601 cgacgaagac gaagatcagg aagagcttga aatgtcgctt gaagagatca tcgaatacgt
    12661 tcgtgttgcc gcgctgttat gccacgacac ctttactcat ccgcaaccga ccgcgccaga
    12721 agtacaaaaa ccgactctac actaaaaaca aaaaacgtaa ggagagtgtt atgagtgaga
    12781 tatcccggca agagtttcag cgtcgccgtc aggccctggt ggagcaaatg caacccggca
    12841 gcgccgcgct gatttttgct gcaccagaag taacacgtag cgccgacagc gaatacccct
    12901 atcgtcagaa cagtgacttc tggtacttca ccggctttaa cgaaccggaa gcggtgctgg
    12961 tgctgattaa aagcgatgac actcataacc acagcgttct gtttaaccgc gttcgcgacc
    13021 tgacggcgga gatctggttt ggccgtcgct taggccagga tgccgcgcca gagaaactgg
    13081 gcgttgaccg cgcactggca ttcagcgaaa tcaatcagca actttatcaa ctacttaacg
    13141 gcctggatgt ggtttaccat gcccagggcg aatatgcata tgctgatgaa atcatgaaca
    13201 gtgcgctgga aaaactgcgt aaaggttcgc ggcaaaatct caccgcaccg gcaacgatga
    13261 tcgactggcg tcctgttgtt catgaaatgc gcctgttcaa atcgccagaa gagattgccg
    13321 tactccgccg cgcgggagaa atcaccgcca tggcacatac acgggcgatg gaaaaatgcc
    13381 gtccgggaat gttcgagtac catctggaag gcgaaattca ccacgaattt aaccgccacg
    13441 gtgcgcgcta tccgtcctat aacaccattg tcggcagcgg tgaaaacggc tgcattctgc
    13501 actacaccga aaacgagagt gaactgcgcg acggtgacct ggtgttgatt gacgcgggtt
    13561 gtgaatacaa aggttacgcg ggcgatatta cccgcacctt cccggtcaac ggcaaattca
    13621 cccaggccca gcgtgaaatc tacgacattg tgctggagtc tctcgaaacc agcctgcgcc
    13681 tgtatcgtcc gggaacttcc attctggaag tcactggtga agtggtgcgc atcatggtta
    13741 gcggcctggt aaaactcggc attctgaaag gtgatgttga tgaactgatc gctcagaatg
    13801 cccatcgtcc tttctttatg catggcctta gccactggtt agggctagat gtccatgacg
    13861 ttggcgttta tggtcaggat cgctcgcgca ttctggaacc gggcatggta ctgaccgtag
    13921 agccagggct gtatattgcg ccggatgcag aagtgccaga acaatatcgc ggtatcggca
    13981 ttcgtattga agacgacatt gtgattaccg aaaccggtaa cgaaaacctc accgccagcg
    14041 tggtgaaaaa gccggaagaa atcgaagcgt tgatggctgc tgcgagaaag caatgagcgt
    14101 aatcatcgtc ggtggcggca tggcgggcgc gacgctggcg ctggctattt cccggttaag
    14161 tcacggggcg ctgccggtac atttgattga agcgactgcg ccagagtcac atgctcatcc
    14221 gggctttgat ggacgagcga tagcgctggc ggcgggtacc tgtcagcaac tggcgcgcat
    14281 cggcgtctgg caatctctgg cggattgcgc aactgccatc accaccgtgc atgtcagcga
    14341 tcgtggtcac gctggatttg tcaccctcgc cgcagaagat taccaactgg cggcgctggg
    14401 acaggttgtc gaattgcaca atgtcgggca acggctgttt gcattgctgc gtaaagcacc
    14461 tggcgtaacg ctgcattgcc ctgatcgcgt ggctaacgtt gcccgtactc agagtcacgt
    14521 tgaagtgacg ctggaaagtg gcgagacgct gacgggccgc gtgctggtag cagctgatgg
    14581 cacccattca gcgttagcca ccgcgtgcgg cgttgactgg cagcaggagc cttacgaaca
    14641 actggccgtg attgccaacg ttgcgacttc cgttgcgcac gaaggccgcg cttttgaacg
    14701 cttcacgcaa catggcccgc tggcgatgtt gccgatgtct gacggacgct gttcgctggt
    14761 ctggtgtcat ccactggaac ggcgcgaaga ggtgctgtcg tggagcgacg agaagttttg
    14821 ccgtgaactc cagtcggcct ttggctggcg acttggaaaa attacccacg ccggtaaacg
    14881 cagtgcttat ccgctggcgt taacccgcgc cgccagagct attacccatc gtaccgtgct
    14941 ggtgggcaat gcggcgcaaa ctctgcaccc gattgccggg caagggttta acctcggtat
    15001 gcgagatgtc atgagccttg cggaaaccct gactcaggcg catgagcgcg gagaagacat
    15061 cggtgattac ggcatattgt gccgctatca gcagcgtcga cagagcgatc gcgaagcaac
    15121 catcggcgtc acggacagcc tggtacatct ttttgccaac cgttgggcac cgctggttgt
    15181 cgggcgcaac atcggactga tgacgatgga attattcacc ccggcacgcg atgtgctggc
    15241 gcagcgcacc ctcggttggg tggcgcgttg aaggcggttt taaggaataa acaatgcaat
    15301 gtgttgatgt agccattgtt ggtggcggca tggtggggct ggcggttgcc tgcggcttac
    15361 aggggagcgg cttacgcgtt gccgtactgg agcagcgcgt accggaacct ctggcggcgg
    15421 atgcaccacc acaactgcgc gtttcggcta tcaatgccgc cagcgaaaaa ttactcaccc
    15481 gtcttggcgt ctggcaggac attctctctc gtagggccag ttgttatcac ggtatggaag
    15541 tgtgggacaa agacagtttt ggtcacattt cgtttgacga tcaaagcatg ggctatagcc
    15601 atcttgggca tatcgttgaa aactcagtga ttcactacgc gctgtggaac aaagcgcagc
    15661 agtcgtcaga tatcactctg ttggcccccg cagaattaca gcaggtcgcc tggggagaaa
    15721 atgaaacttt cctgacgctg aaagatggca gcatgttaac ggcgcgtctg gtgattggcg
    15781 cggacggtgc taattcctgg ttgcgcaaca aagcagatat ttcgctgact ttctgggatt
    15841 atcagcatca cgcgctggta gcgaccattc gcacggaaga accgcatgat gcggtggcgc
    15901 ggcaggtttt ccatggcgaa ggcattctgg cctttttacc gcttagcgat ccgcatcttt
    15961 gctcaattgt ctggtcactg tcgccagagg aagcgcagcg gatgcagcag gcaagtgaag
    16021 atgaatttaa tcgcgcgtta aatatcgctt ttgataatcg cctgggctta tgcaaggttg
    16081 agagcgcgcg tcaggtgttc ccactgacgg ggcgttatgc gcgccagttt gccgcgcacc
    16141 gtctggtgct agtgggcgac gccgcgcata ccattcaccc gctggcgggg cagggagtaa
    16201 atctcggctt tatggatgct gcagagctga ttgctgaact gaaacggttg catcgtcagg
    16261 ggaaagacat cgggcagtac atttacctgc gccggtatga gcgtagccgc aagcacagtg
    16321 cggcgctgat gctggctggt atgcagggat tccgcgatct gttttccggt accaatccgg
    16381 cgaaaaaact gctgcgtgat attggtttga aactggccga cacgcttcct ggcgttaaac
    16441 cacaacttat ccgccaggca atgggattaa acgatttgcc tgaatggctg cgttaaaaat
    16501 ttctcttctg ctgtttattt gatacccatc acactttcat ctcccggttt tttcgccggg
    16561 agattttcct catttgaaat aaactaattt cacctccgtt ttcgcattat attttctaat
    16621 gtcattattt tttgatttag tgttttttga cattttttta gctcttaata ttgccttatt
    16681 caaattagct ttctaatggc atcatcttta tatagaaact ggtgtgtttt ttggtttttt
    16741 attctgtcgc gattttggcg ttttttaacc ataagctaat gtgatgatca attttacctt
    16801 atggttaaca gtctgtttcg gtggtaagtt caggcaaaag agaacgattg cgttggggac
    16861 cgggagtggc tccgatgctg ggtttcgtgg tgataatttc accatgaaaa gttgtcagcc
    16921 ccgcttattc aatgaggaca agatggcaca acagactcct ttgtacgaac aacacacgct
    16981 ttgcggcgct cgcatggtgg atttccacgg ctggatgatg ccgctgcatt acggttcgca
    17041 aatcgacgaa catcatgcgg tacgtaccga tgccggaatg tttgatgtgt cacatatgac
    17101 catcgtcgat ctccgcggca gccgcacccg ggagtttctg cgttatctgc tggcgaacga
    17161 tgtggcgaag ctcaccaaaa gcggcaaagc cctttactcg gggatgttga atgcctctgg
    17221 cggtgtgata gatgacctca tcgtctacta ctttactgaa gatttcttcc gcctcgttgt
    17281 taactccgcc acccgcgaaa aagacctctc ctggattacc caacacgctg aacctttcgg
    17341 catcgaaatt accgttcgtg atgacctttc catgattgcc gtgcaagggc cgaatgcgca
    17401 ggcaaaagct gccacactgt ttaatgacgc ccagcgtcag gcggtggaag ggatgaaacc
    17461 gttctttggc gtgcaggcgg gcgatctgtt tattgccacc actggttata ccggtgaagc
    17521 gggctatgaa attgcgctgc ccaatgaaaa agcggccgat ttctggcgtg cgctggtgga
    17581 agcgggtgtt aagccatgtg gcttgggcgc gcgtgacacg ctgcgtctgg aagcgggcat
    17641 gaatctttat ggtcaggaga tggacgaaac tatttctcct ttagccgcca acatgggctg
    17701 gactatcgcc tgggaaccgg cagatcgtga ctttatcggt cgtgaagccc tggaagtgca
    17761 gcgtgagcat ggtacagaaa aactggttgg tctggtgatg accgaaaaag gcgtgctgcg
    17821 taatgaactg ccggtacgct ttaccgatgc gcagggcaac cagcatgaag gcattatcac
    17881 cagcggtact ttctccccga cgctgggtta cagcattgcg ctggcgcgcg tgccggaagg
    17941 tattggcgaa acggcgattg tgcaaattcg caaccgtgaa atgccggtta aagtgacaaa
    18001 acctgttttt gtgcgtaacg gcaaagccgt cgcgtgattt acttttttgg agattgattg
    18061 atgagcaacg taccagcaga actgaaatac agcaaagaac acgaatggct gcgtaaagaa
    18121 gccgacggca cttacaccgt tggtattacc gaacatgctc aggagctgtt aggcgatatg
    18181 gtgtttgttg acctgccgga agtgggcgca acggttagcg cgggcgatga ctgcgcggtt
    18241 gccgaatcag taaaagcggc gtcagacatt tatgcgccag taagcggtga aatcgtggcg
    18301 gtaaacgacg cactgagcga ttccccggaa ctggtgaaca gcgaaccgta tgcaggtggc
    18361 tggatcttta aaatcaaagc cagcgatgaa agcgaactgg aatcactgct ggatgcgacc
    18421 gcatacgaag cattgttaga agacgagtaa cggctttatt cctcttctgc gggagaggat
    18481 cagggtgagg aaaatttatg cctcaccctc actctcttcg taaggagaga ggttcacaat
    18541 tcactgcacg tttcaggaac catcgctcat gacacagacg ttaagccagc ttgaaaacag
    18601 cggcgctttt attgaacgcc atatcggacc ggacgccgcg caacagcaag aaatgctgaa
    18661 tgccgttggc gcacaatcgt taaacgcgct gaccggccag attgtaccga aagatattca
    18721 gcttgcgacc ccgccgcagg ttggcgcacc ggcgaccgaa tacgccgcac tggcagaact
    18781 caaggctatt gccagtcgca ataaacgctt cacgtcttac atcggcatgg gttacaccgc
    18841 cgtgcagcta ccgccggtta tcctgcgtaa catgctggaa aatccgggct ggtataccgc
    18901 gtacactccg tatcaacctg aagtctccca gggccgcctt gaagcactgc tcaacttcca
    18961 gcaggtaacg ctggatttga ctggactgga tatggcctct gcttctcttc tggacgaggc
    19021 caccgctgcc gccgaagcaa tggcgatggc gaaacgcgtc agcaaactga aaaatgccaa
    19081 ccgcttcttc gtggcttccg atgtgcatcc gcaaacgctg gatgtggtcc gtactcgtgc
    19141 cgaaaccttt ggttttgaag tgattgtcga tgacgcgcaa aaagtgctcg accatcagga
    19201 cgtcttcggc gtgctgttac agcaggtagg cactaccggt gaaattcacg actacactgc
    19261 gcttattagc gaactgaaat cacgcaaaat tgtggtcagc gttgccgccg atattatggc
    19321 gctggtgctg ttaactgcgc cgggtaaaca gggcgcggat attgtttttg gttcggcgca
    19381 acgcttcggc gtgccgatgg gctacggtgg cccacacgcg gcattctttg cggcgaaaga
    19441 tgaatacaaa cgctcaatgc cgggccgtat tatcggtgta tcgaaagatg cagctggcaa
    19501 taccgcgctg cgcatggcga tgcagactcg cgagcaacat atccgccgtg agaaagcgaa
    19561 ctccaacatt tgtacttccc aggtactgct ggcaaacatc gccagcctgt atgccgttta
    19621 tcacggcccg gttggcctga aacgtatcgc taaccgcatt caccgtttga ccgatatcct
    19681 ggcggcaggc ctgcaacaaa aaggtctgaa gctgcgccat gcgcactatt tcgacactct
    19741 gtgtgtggaa gtggccgaca aggcgggcgt actggcgcgt gccgaagcgg ctgaaatcaa
    19801 cctgcgtagc gatatcctga acgcggttgg gatcaccctt gatgaaacca ccacgcgtga
    19861 aaacgtaatg cagcttttca gcgtgctgct gggcgataac cacgggctgg agatcgacac
    19921 gctggacaaa gacgtggctc acgacagccg ctctattcag cctgcgatgc tgcgcgacga
    19981 cgaaatcctc actcatccgg tgtttaatcg ctaccacagc gaaaccgaaa tgatgcgcta
    20041 tatgcactcg ctggagcgta aagatctggc gctgaatcag gcgatgatcc cgctgggttc
    20101 ctgcaccatg aaactgaacg ccgccgccga gatgatccca atcacctggc cggaatttgc
    20161 cgaactgcac ccgttttgcc cgccggaaca ggctgaaggt tatcagcaga tgattgcgca
    20221 gctggctgac tggctggtga aactgaccgg ttacgacgcc gtttgtatgc agccgaactc
    20281 cggcgcacag ggcgaatacg cgggcctgct ggcgattcgt cattatcatg aaagccgcaa
    20341 cgaagggcat cgcgatatct gcctgatccc ggcttctgcg cacggaacta accccgcttc
    20401 tgcacatatg gcaggaatgc aggtggtggt tgtggcgtgt gataaaaacg gcaacatcga
    20461 tctgactgat ctgcgcgcga aagcggaaca ggcgggcgat aacctctcct gtatcatggt
    20521 gacttatccc tctacccacg gcgtgtatga agaaacgatc cgtgaagtgt gtgaagtcgt
    20581 gcatcagttc ggcggtcagg tttaccttga tggcgcgaac atgaacgccc aggttggcat
    20641 cacctcgccg ggctttattg gcgcggacgt ttcgcacctc aacctgcata aaactttctg
    20701 cattccgcac ggcggtggtg gtccgggtat gggaccgatc ggtgtgaaag cgcatctggc
    20761 accgtttgta ccgggtcata gcgtggtgca aatcgaaggc atgttaaccc gtcagggcgc
    20821 agtttctgcg gcaccgttcg gtagcgcctc tatcctgcca atcagctgga tgtacatccg
    20881 catgatgggc gcagaagggc tgaaaaaagc aagccaggtg gcaatcctca acgccaacta
    20941 tattgccagc cgcctgcagg atgccttccc ggtgctgtat accggtcgcg acggtcgcgt
    21001 ggcgcacgaa tgtattctcg atattcgccc gctgaaagaa gaaaccagca tcagcgagct
    21061 ggatattgcc aagcgcctga tcgactacgg tttccacgcg ccgacgatgt cgttcccggt
    21121 ggcgggtacg ctgatggttg aaccgactga atctgaaagc aaagtggaac tggatcgctt
    21181 tatcgacgcg atgctggcta tccgcgcaga aattgaccag gtgaaagccg gtgtctggcc
    21241 gctggaagat aacccgctgg tgaacgcgcc gcacattcag agcgaactgg tcgccgagtg
    21301 ggcgcatccg tacagccatg aagttgcggt attcccggca ggcgtggcag acaaatactg
    21361 gccgacggtg aaacgtctgg atgatgttta cggcgaccgt aacctgttct gctcctgcgt
    21421 accgattagc gaataccagt aattcactga ttcgactatt ttctaaaggc gcttcggcgc
    21481 ctttttagtc agatgacaaa gtacaaaagt gctcagacag tcccctcgcc cctttgggga
    21541 gagggttagg gtgaggggaa caggccggca ctggcgcgaa tatttaccct caccccggcc
    21601 ctctccctga aagggcgagg ggaaaagcgt gccaacattg aagattgagc cagtttgtta
    21661 gcaatctcaa agatacgtca acgaattaat ttttctcgga aaaacaaatg gctatagcac
    21721 ttgtgaccgg tggcagtcgc ggcatcgggc gggcaactgc attactgttg gcgaaagaag
    21781 agtatacggt ggcggttaat tatcaacaaa acctccatgc ggcgcaggaa gtggtgaact
    21841 taatcacgca agccggtggc aaggcattcg tgctccaggc ggatatcagc gacgaaaatc
    21901 aggtcatcgc aatgtttaca gcaatcgatc agcacgatga accgctagca gcgctggtca
    21961 ataacgccgg gatcttgttt acccagtaca ccgttgaaaa ccttaccgca gagcgaatca
    22021 accgagtact ttccaccaac gtgacgggat attttctctg ctgccgcgag gcggtaaaac
    22081 gcatggcgct taaaaatggt ggcagtggcg gcgctatcgt caatgtctct tcggtggcct
    22141 cacggttggg ttcgccaggg gaataggttg attacgcggc atcgaaaggg gcgattgata
    22201 cgttaaccac cggactatcg ctggaagtcg ccgcgcaggg gatccgcgtt aactgcgtgc
    22261 ggccagggtt tatttatacc gaaatgcacg ccagcggcgg cgagcttgga cgcgtcgatc
    22321 gcgttaagtc gaacatcccc atgcagcgtg gtggacaggc agaagaggtc gcgcaggcca
    22381 ttgtctggct actaagtgat aaagcctctt acgtcacggg aagttttatc gatttggcgg
    22441 gcgggaaata aaacagggaa gttgtctgac cggatgcaac aagtattgca tccggtactt
    22501 catcgactta aagcttctcg ccgttgctgg caatcacctc tttgtaccag ttaaagctct
    22561 tcttacgtga acgcgacata tcgccagtac cgtcgtcatg tttattcaca tagataaagc
    22621 cgtagcgttt gctgtactgc ccggtagtga atgacacgca gtcgatgcag ccccacggtg
    22681 tgtagcccat cagatccaca ccatcgtaag tcactgcttt tttcatctct tcaatatggg
    22741 cgcgcaggta gtcaatgcgg tagtcgtcgt tgatgctgcc atcttcttcc actttgtcgt
    22801 aagcgccaaa accgttttcg acaataaaca gcggcctctg ataacgctca tacagttcgc
    22861 aaagggcata gcgcagacct actggatcaa tctgccagca ccagtcagac gctttaacat
    22921 acgggttcgg tacgctgcct tcaaaaccag agatcgcatc gccggtgccg ccttcggcct
    22981 tcactgcgtt gatcatgtaa tagctgaaac caagataatc gcaggtgcct tcacgcagta
    23041 catccagatc gccatcttcc attttgatgt taaatccgcg acgctcccac tcgttcaaca
    23101 cataggacgg gtaatagccg cgtagctgca catcggtaaa gacgtagcgt tcgcgcatcg
    23161 actcctgagc gaacatcaca tcgtccgggt tacaggagta aggatagagc ggcaccatcg
    23221 ccagcataca gccgactttc atctccgggt taatgcgacg cgcggctttc actgctaggg
    23281 cgctggcgac aaactggtga tgcagcacct ggtacatcgt ctcttccggg ttgtcatgct
    23341 cggtatacac cacgccggag cagcagtaac caaacagcgg cgcacgccag ttacgctggt
    23401 tgtttatttc gttgaaggtc atccagtatt tgactttatg cttgtagcgt tcgaatacca
    23461 cttccgcgaa acggacaaag aaatcaacga ctttacggtt ggtccagcta ccgtattgct
    23521 gcaccagatg cagcggtatt tcaaagtggg agagggttat caccggttcg atgttgtatt
    23581 tcagcagttc atcgaacaga gagtcgtaaa acttcagccc ttcttcgttt ggctgagctt
    23641 catcaccttt tggaaaaata cgcgtccagg cgatggaagt ccgaaaacat ttgaagccca
    23701 tttcggcaaa tagcttgatg tcttccttgt agtgaccata aaaatcaacg gcttcatggt
    23761 ttggatagta ttttcctggc aagacttctt tggtgatttc gcgcggcacg ccgtgtgcgc
    23821 caccggtcag aacgtcacaa atgctcggtc ctttgccgcc tttgttccag ccgccttcga
    23881 cctgatgagc ggcaacggcg ccaccccata agaaatcttt cggtaaggtg agttttttca
    23941 ctatcatagg ctccagatga gtcaattctt acaggaaata gtaccatgca gccaaacgac
    24001 atcacttttt ctcaacgttt ccaggatgac attctggctg ggcgtaaaac catcaccatc
    24061 cgcgacgagt ctgaatcgca cttcaaaacg ggtgatgtgc ttcgtgtcgg acgttttgaa
    24121 gatgacggtt atttttgcac gattgaagtc accgcaacct caaccgtaac gctggatacg
    24181 ctgacggaaa aacatgcaga gcaggaaaat atgaccctga ctgaactgat aaaggtcatt
    24241 gccgacatct atcccggtca gacacagttt tatgtgattg aatttaaatg tctttaaatt
    24301 gaactcacgc ctgttagttg aaatttctca tttaatttca tgatttttag gagattagtc
    24361 tcaaatcaga atttatttta gctaacaggt gttcactgga actattctca gttacgctgg
    24421 agaggtatac atgtgcgtgt acgtttctcc ggagtaagtt atggttcaga agcccctcat
    24481 taagcaggga tattcgctgg cagaggaaat tgccaacagc gtcagtcacg gcattgggtt
    24541 ggtgtttggt atcgttgggc tggtgttgct actggttcag gcggtggatc tcaatgccag
    24601 cgccacagcg ataaccagct acagcctcta tggcggcagt atgatcctgc tgttcctcgc
    24661 ttcgacgctc tatcacgcca ttcctcatca acgggcaaaa atgtggctga agaaatttga
    24721 ccattgcgct atttacctgt tgattgccgg aacctacacg ccgtttttgc tggtggggct
    24781 ggattctccg ttagcgcgcg ggttgatgat tgttatctgg agcctggcat tgctggggat
    24841 tctgtttaaa ctgaccatcg cgcaccgatt caaaatttta tctctggtga cctatctggc
    24901 gatgggctgg ctgtcgctgg tggtaattta tgaaatggca gttaagctcg cggcgggcag
    24961 cgttacctta ctggcggtag gcggcgtggt ttattcgctc ggagtgattt tctatgtctg
    25021 caaacgcatt ccctacaatc atgccatctg gcacggcttc gtgctcggcg gcagtatgtg
    25081 ccactttctg gcgatctatt tgtatattgg gcaggcgtaa tgggtatgcc tttgtaggcc
    25141 tgataagacg cgccagcgtc gcatcaggca tcggagcgca accgccggat gcggcgtgaa
    25201 cgccttatcc gatctacggg cctcgtccct gttgtgataa aggcatcaaa tcccagacat
    25261 gataagacgc gtcagcgtcg catcaggcga taaatgcctg atgcgataca gagatttact
    25321 cttcgagtga atacggcagc ggctcgatat gcagtgtatt cgcatcgtcg cgtacgcgga
    25381 agatgctgtc cggttccata tcgttattca tgacgacctg taccacgacc tgaccatctt
    25441 ccagttttac cgcagccagc accgtaccgg tacgacgcca gttctcgccc attttcagct
    25501 ctaagtcttc accagcttcc ggcagtcggc tggcgctacc tgccagcaac cagagcgcac
    25561 gtttattggc accacggaat tttgctcgcg ccaccatctc ttgtccggta taacagcctt
    25621 tcttaaaact gataccgccc agcgcctgga gattggtcgc ctgtgggata aactgcccgc
    25681 tgttcgcggc atcaatcacc gggaaacccg cttcaatgtt taatgccagc cactgttggc
    25741 tattgttcag ttccgcttca ccgcgcagtt tatcggtcag catattggca gtagcttcat
    25801 cggttacgat caggaaacgt tctgccgggt gttcaaacca tagcaaagtg gtcgcgcctt
    25861 ctttaactac ctgtttttct ctcgaaggta gttcactaaa gagattggcg agcgcggcgc
    25921 gcgcctgaaa accggcaaca ccaagcagca cacgctcgtc gtctgccgcg atggtcactt
    25981 tagagaacac cgcatatttt ttcagttcag tcagctgcgg ttcacgcacg ctgcgccgtt
    26041 caatccacgc aaagccatcg ccgtcgcgga acagacgtaa attgctccac attttacctt
    26101 tggcgtcgca atgggcggcg agcaggtgct gattttctgt catctggctg acatctgctg
    26161 tcacctgacc ctgcatatat ttttcgctgt ccgcgccagt aatggtggca agcgcccagt
    26221 catcaagcgt catcagcgtc agcggtaaac gggcagaagc cgtaggctga cggggaggaa
    26281 aaggtgtaaa agccataata gagtcctgag ttgcttaacg gcgtattaat aactaatggt
    26341 aaaagagcta tgaggcaatg caagtgcttt cactacgcga tctgtgcctt ctgcgaggcg
    26401 acttccaaaa aaatgaagct cttgaattgt tgtgaattaa tttcgcgatc gcgatggtgt
    26461 ttactgatat tcccgtaaaa gaagtcgaaa aacacgttac aatagccggg tactggattt
    26521 tcgtgagaca ggaagaagat ggacattaac aacaaagccc gcattcattg ggcatgccgc
    26581 cgtggtatgc gcgaactcga tatttcaatc atgccgtttt tcgaacatga gtacgacagc
    26641 ttaagcgatg acgaaaaacg catctttatt cgtctgctgg aatgtgacga tccggacctg
    26701 tttaactggc tgatgaatca cggtaaaccg gccgatgcag aactggaaat gatggtccga
    26761 ctcatccaga cacggaaccg ggaacgtggt cctgtggcaa tctgatttgc gcgtctcctg
    26821 gcgcgcacag tggctttcct tgctgattca tgggctggtt gccgctgtta ttttactcat
    26881 gccctggccg ctcagttaca ccccgttatg gatggtgtta ctttcgctgg tggtatttga
    26941 ttgcgttcgc agccagcggc gtattaatgc tcgccagggg gaaattcgat tgttgatgga
    27001 cgggcgtttg cgttggcaag ggcaggagtg gagcatcgtc aaagcaccgt ggatgattaa
    27061 gagcggcatg atgctgcgtt tacgttctga tggcggtaaa cggcaacatt tatggctggc
    27121 agccgacagc atggacgaag ctgaatggcg ggatttacgg cggattttgt tgcaacaaga
    27181 gacgcaaaga taaatatgac ggcaaagcat atgcgctaag ccgtcagtga gatcaggcgt
    27241 aatgctctgc catttcgttg aggatttgct cgcaccagct ctgaatacgc tcgtcgctaa
    27301 ggtcatactg gttagtttca tccagcgcca gacccacgaa cagttgcccg tcagcaatca
    27361 ccggtttcgg gctggtaaat tcatatcctt ccgttggcca gtagccgacg aacttcacgc
    27421 ctttggtcga gagtttgtca tgcagcatac cgagcgcatc gaggaaccac tcgccgtatc
    27481 ccagttgatc gccaagccca tacagcgcaa caattttacc ttcaaggttc aggtcgtcga
    27541 gctgatccca gacggcttcc cagtcttcct ggatttcgcc aaaatcccag gtcgggatac
    27601 ccagaatgag cacatcgtac tgctccatta atttcgggga gtcgtccttg aggttatgta
    27661 aggtcaccag ttctgggccg ataatatcgc ggattttttc tgccgccatt tcggtgtaac
    27721 aggtgctgga accgtaaaaa agacccatat tcataacgta agcttctcga ttctgctgtg
    27781 gcttttgcgg ctagtgtatc agaatcgctt tagtcacggc acaataaggc ataatggatc
    27841 aaatgagtga ccataagggg cgcaagtgaa acaggatctg gcacgcatcg agcagtttct
    27901 tgatgctctg tggctggaaa aaaatctggc tgaaaatacg ttgaacgctt accgtcgcga
    27961 tctgtcaatg atggtggagt ggttgcatca ccgcgggttg acgctggcga cggcgcaaag
    28021 tgacgatttg caggcattac tggcagaacg gctagagggc gggtataaag ccaccagctc
    28081 agcgcgtttg ctgagtgcag tgcgccgatt gttccagtat ctttatcgcg aaaagtttcg
    28141 tgaagacgat cccagtgcgc atctcgcttc accgaaattg ccacagcgtt tgccaaaaga
    28201 tttaagcgaa gcgcaggtcg aacgtttatt acaggcacca ttaattgatc agccactgga
    28261 gctacgcgat aaagccatgc ttgaagtgtt gtatgctacc gggctgcgtg tctctgaact
    28321 ggtcgggctg acaatgagtg atatcagcct acgtcagggc gtagtgcggg tcattggtaa
    28381 aggcaacaaa gagcgcctgg tgccgttagg tgaagaggcc gtttactggc tggaaaccta
    28441 tctggaacat gggcgtccgt ggctgttgaa tggtgtgtca attgacgtgt tgtttcccag
    28501 ccagcgtgcg cagcagatga cgcgacagac cttctggcac cgcattaaac attatgccgt
    28561 gctggcgggt atcgacagcg aaaagctgtc accgcatgtg ttgcgtcacg cttttgccac
    28621 tcatttatta aatcatggtg cggatttacg cgtggtgcag atgctactgg gccacagcga
    28681 tctctccacc acgcaaattt atacgcatgt cgctaccgag cgtctgcggc aacttcatca
    28741 acagcatcac ccgcgggcgt gatgtctgaa aagaacggga agatttatga agaaaggttt
    28801 tatgttgttt actttgttag cggcgttttc aggctttgct caggctgatg acgcggcaat
    28861 tcaacaaacg ttagccaaaa tgggcattaa aagcagcgat attcagccag cacccgtggc
    28921 tggcatgaag actgtgctga ctaacagcgg tgtgttgtac atcaccgatg acggtaaaca
    28981 tatcattcag gggccaatgt atgacgttag tggcgcggct ccggtcaatg tcaccaataa
    29041 aatgctgcta aagcagttga atgcgcttga aaaagagatg atcgtttata aagcgccgca
    29101 ggaaaaacac gtcatcaccg tgtttactga tattacctgt gggtactgcc acaaactgca
    29161 tgagcaaatg gcagactaca acgcgctggg gatcaccgtg cgttatcttg ctttcccgcg
    29221 ccaggggctg gagagccagg ctgagcaaca aatgaaagcc atctggtgtg cgaaagataa
    29281 aaacaaagcg tttgatgatg tgatggctgg taaagctgcc actccggcaa gttgcgatat
    29341 cgatattgcc gatcattacg cacttggcgt ccagcttggc gttagcggca ctccggcaat
    29401 tgtgctgagc aatggcacac ttgttccggg ttaccagccg ccgaaagaga tgaaagaatt
    29461 cctcgacgaa caccaaaaaa tgaccagcgg taattaattc gcgtgaaaca acagatacaa
    29521 cttcgtcgcc gtgaagtcga tgaaacggca gacttgcccg ctgaattgcc tcccttgctg
    29581 cgccgtttat acgccagccg gggagtacgc agtgcgcaag aactggaacg cagtgttaaa
    29641 ggtatgttgc cctggcagca actaagcggt gtcgaaaagg ccgttgagat cctttacaac
    29701 gcttttcgcg aaggaacgcg gattattgtg gtcggtgatt tcgacgccga cggcgcgacc
    29761 agcacggctc taagcgtgct ggcgatgcgc tcgcttggtt gcagcaatat cgactacctg
    29821 gtaccaaacc gtttcgaaga cggttacggc ttaagcccgg aagtggtcga tcaggcccat
    29881 gcccgtggcg cgcagttaat tgtcacggtg gataacggta tttcctccca tgcgggggtt
    29941 gaacacgctc gctcgttggg cattccggtt attgttaccg atcaccattt gccaggcgaa
    30001 acattacccg cggcggaagc gatcattaac cctaacttgc gcgactgtaa tttcccgtcg
    30061 aaatcactgg caggcgtggg tgtggcgttt tatctgatgc tggcgctgcg cacctttttg
    30121 cgcgatcagg gctggtttga tgagcgcggc atcgcaattc ctaacctggc agaactgctg
    30181 gatctggtcg cgctggggac agtggcggac gtcgtgccgc tggacgctaa taatcgcatt
    30241 ctgacctggc aggggatgag tcgcatccgt gccggaaagt gccgtccagg gattaaagcg
    30301 ctgctggaag tggcaaaccg tgatgcacaa aaactcgccg ccagcgattt aggttttgcg
    30361 ctggggccac gtctcaatgc tgccgggcga ctggacgata tgtccgtcgg tgtggcgctg
    30421 ttgctgtgcg acaacatcgg cgaagcgcgc gtgctggcaa atgaactcga tgcgctaaat
    30481 cagacgcgaa aagagatcga acaaggaatg cagattgaag ccttgaccct gtgcgagaaa
    30541 ctggagcgaa gtcgcgacac gctacccggc gggctggcaa tgtatcaccc cgaatggcat
    30601 cagggcgttg tcggtattct ggcttcgcgc atcaaagagc gttttcaccg tccggttatc
    30661 gcctttgcgc cagcaggtga tggtacgctg aaaggttccg gtcgctccat tcaggggctg
    30721 catatgcgtg atgcgctgga gcgattagac acactctacc ccggtatgat gctgaagttt
    30781 ggcggtcatg cgatggcggc gggtttgtcg ctggaagagg ataaattcga actctttcaa
    30841 caacggtttg gcgagctggt taccgagtgg ctggaccctt cgctattgca aggcgaagtg
    30901 gtgtcagacg gcccgttaag cccggccgaa atgaccatgg aagtggcgca gctgctgcgc
    30961 gatgctggcc cgtgggggca gatgttcccg gagccgctgt ttgatggtca tttccgtctg
    31021 ctgcaacagc ggctggtggg cgaacgtcat ttgaaagtca tggtcgaacc ggtcggcggc
    31081 ggtccgctgc tggatggtat tgcttttaat gtcgataccg ccctctggcc ggataacggc
    31141 gtgcgcgaag tgcaactggc ttacaagctc gatatcaacg agtttcgcgg caaccgcagc
    31201 ctgcaaatta tcatcgacaa tatctggcca atttagcgtc atcttctcta taaaaaagag
    31261 cgtggattgg gtacaatccc gctcttatca ccgcattttg actagctcaa taaaagaaat
    31321 cagaccatgt ttgaaattaa tccggtaaat aatcgcattc aggacctcac ggaacgctcc
    31381 gacgttctta gggggtatct ttgactacga cgccaagaaa gagcgtctgg aagaagtaaa
    31441 cgccgagctg gaacagccgg atgtctggaa cgaacccgaa cgcgcacagg cgctgggtaa
    31501 agagcgttcc tccctcgaag ccgttgtcga caccctcgac caaatgaaac aggggctgga
    31561 agatgtttct ggtctgctgg aactggctgt agaagctgac gacgaagaaa cctttaacga
    31621 agccgttgct gaactcgacg ccctggaaga aaaactggcg cagcttgagt tccgccgtat
    31681 gttctctggc gaatatgaca gcgccgactg ctacctcgat attcaggcgg ggtctggcgg
    31741 tacggaagca caggactggg cgagcatgct tgagcgtatg tatttgcgct gggcagaatc
    31801 gcgtggtttc aaaactgaaa tcatcgaaga gtcggaaggt gaagtggcgg gtattaaatc
    31861 cgtgacgatc aaaatctccg gcgattacgc ttacggctgg ctgcgtacag aaactggcgt
    31921 tcaccgcctg gtgcgtaaga gcccgtttga ctccggcggt cgtcgccaca cgtcgttcag
    31981 ctccgcgttt gtttatccgg aagttgatga tgatattgat atcgatatca acccggcgga
    32041 tctgcgcatt gacgtttatc gcgcgtccgg cgcgggcggt cagcacgtta accgtaccga
    32101 atctgcggtg cgtattaccc acatcccgac cgggatcgtg acccagtgcc agaacgaccg
    32161 ttcccagcac aagaataaag accaggccat gaagcagatg aaagcgaagc tttatgaact
    32221 ggagatgcag aagaaaaatg ctgagaaaca ggcgatggaa gataacaaat ccgacatcgg
    32281 ctggggcagc cagattcgtt cttatgtcct tgatgactcc cgcattaaag atctgcgcac
    32341 cggggttgaa acccgcaata cgcaggccgt gctggacggc agcctggatc aatttatcga
    32401 agcaagtttg aaagcagggt tatgaggaac caacatgtct gaacaacacg cacagggcgc
    32461 tgacgcggta gtcgatctta acaatgaact gaaaacgcgt cgtgagaagc tggcgaacct
    32521 gcgtgagcag ggaattgcct tcccgaacga tttccgtcgc gatcatacct ctgaccaatt
    32581 gcacgcagaa ttcgacggta aagagaacga agaactggaa gcgctgaaca tcgaagtcgc
    32641 cgttgctggc cgcatgatga cccgccgtat tatgggtaaa gcctcttttg ttaccctgca
    32701 ggacgttggc ggtcgtattc agctgtacgt tgcccgtgac gatcttccgg aaggcgttta
    32761 caacgagcag ttcaaaaaat gggatctcgg cgacatcctc ggcgcgaaag gtaaactgtt
    32821 caaaaccaaa accggcgaac tgtctatcca ctgcaccgag ctgcgtctgc tgaccaaagc
    32881 actgcgtccg ctgccggaca aattccacgg cttgcaggat caggaagcgc gctatcgtca
    32941 gcgttatctg gatctcatct ctaacgatga atcccgcaac acctttaaag tgcgttcgca
    33001 gatcctctct ggcattcgcc agttcatggt aaatcgcggc tttatggaag ttgaaacgcc
    33061 gatgatgcag gtgatcccag gcggtgccgc tgcgcgtccg tttatcaccc atcataacgc
    33121 gctggatctt gacatgtacc tgcgtatcgc gccggaactg tacctcaagc gtctggtggt
    33181 cggtggcttc gagcgtgtat tcgaaatcaa ccgtaacttc cgtaacgaag gtatttccgt
    33241 acgtcataac ccagagttca ccatgatgga actctatatg gcttacgcag attacaaaga
    33301 tctgatcgag ctgaccgaat cgctgttccg tactctggca caggatattc tcggtaagac
    33361 ggaagtcacc tacggcgacg tgacgctgga cttcggtaaa ccgttcgaaa aactgaccat
    33421 gcgtgaagcg atcaagaaat atcgcccgga aaccgacatg gcggatctgg acaacttcga
    33481 ctctgcaaaa gcgattgctg aatctatcgg catccacgtt gagaagagct ggggtctggg
    33541 ccgtatcgtt accgagatct tcgaagaagt ggcagaagca catctgattc agccgacctt
    33601 cattactaaa tatccggcag aagtttctcc gctggcgcgt cgtaacgaca ttaacccgga
    33661 aatcacagac cgctttgagt tcttcatcgg tggtcgtgaa atcggtaacg gctttagcga
    33721 gctgaatgac gcggaagatc aggcgcagcg cttcctggat caggttgccg cgaaagacgc
    33781 aggtgacgac gaagcgatgt tctacgacga agattacgtc accgcactgg aacacggctt
    33841 accgccgaca gcaggtctgg gaattggtat cgaccgtatg gtaatgctgt tcaccaacag
    33901 ccataccatc cgcgacgtta ttctgttccc ggcgatgcgt ccagtaaaat aagcattacg
    33961 ttatgctaat aaccccggca aatgtcgggg ttttttaatt gtgctgcgcg aaagcagaca
    34021 acaattttct tgcttcacta ttggctgcct gcattaccat ccacggactg aacgcccacg
    34081 gcgtggcatc aataccgtgt aatacatctg ctaaatcaca ccattgataa tccatcactt
    34141 catcatcgtt gatctgtaac gcactgttgg tgcgtgcggc aaataccgga cacacttcat
    34201 tttccacaat gccattcgga tcggtggcgc gatagcgaaa gtcaggatag atagattcag
    34261 gaggcgtaat ttccacgcca agctcataac ggcaacggcg gatcactgcg tcttcgttgc
    34321 tttctcccag ttgtgggtgc ccacaaaccg agttagtcca cacgccaggc catgcttttt
    34381 tgctcagtgc gcggcgggta actaataatt gccccttggc attaaacagc caactggaga
    34441 acgcgagatg taagagggtg tctgccgtgt gtgcggcata cttttccagc gtacccgtgg
    34501 gaactccctg tgcattcaat aaaatgacgt gttccgtttg cataatttct cacatgtaat
    34561 tctgatcact catcattata gataattgaa gagtgaaatg attaaccaag tgagcgttta
    34621 tcgacaaccg cccgttttga gcggatgccg acaggtaaaa accatttaaa aaagtagcat
    34681 catatatttg aagcgacttt caatggccat tgcaacacag tcagaaatga caggaataga
    34741 gagagtaaaa agcaacagca gactgaatat agcgcgcatt aaaagattta tattattctc
    34801 catgctcatt tttcttcagc ttgcgcatca gaagccacat acgtactgtc ctgactttta
    34861 aatctttatc agagacggat tccttcacca ggtcagcatg atgctgatag ccattaaaga
    34921 agacgttcag cacaacggcg ctaagggttg cgagcataat gccgctatgt agcagcggtt
    34981 gcagtacggc cggtaactta gaaaagaaat cgtgagagag cgtcggagtc atgccaacgc
    35041 cgagactgat cgccacaata tagaggttgt aacggttggt ggtgtagtta cagcgcgaca
    35101 gaattcgaat ccctgtcgcc agtaccatgc cgaacatcac tagaccagcg ccgcccagca
    35161 caaattgcgg aatggaggct accagcaccg ccatttttgg caccatgcca aacaggatta
    35221 atataattcc cgaagaaata cacacccagc ggctatgaac gcgcgtcacg ctaaccaggc
    35281 caacgttttg agaaaatgac gtgtggggga agctgttaaa cgtgccgcct atcattgtcc
    35341 ctacgccatc gacacgcagc ccgcgaataa tatcgtgtga agagagttta cgaccgacta
    35401 tttcacccag tgccaggaac atccccatcg actcgataaa cacgatgatt aacacggcag
    35461 tcatggtcag aatggaaacg ggatcgaaaa tcggcatacc aaacgacatc ggggtgacaa
    35521 tcgcaaacca caaggcatca tgtagcccgg ataaattgac ttcattcatc atccacgaaa
    35581 gtaaaaagcc aaatacaatc cccagtaata cggcgacgtt ggacataaat cctttcgcat
    35641 agcgagtaat gagcaagata aaaattaaga cggcaaagga gatacctgaa taaacgggat
    35701 taccatattg cggattccct ttacctccgg cggcccagtc aatacccacc tgaatgatgc
    35761 taagtccgac agaagtaata accacaccgg taaccagtgg cgggaataaa ggcatcaagc
    35821 gaccgataag tggcgctaat aatgtggtga taaaacccgc ggcgatagtg gcaccaaata
    35881 tccccagcag gccaatatcc gggttcatcc caatggctat cattggtgtt acagcagcaa
    35941 aggtcaccga cataatcacc ggcaggcgga tccccataaa gcggccgata ccgatacatt
    36001 gcaataatgt gacgatcccg cagcaaaaga gatccgagct aatgagcatc gcaatagctt
    36061 cttttgagag gcccagtcgg tcaccaatca taagaggaac agcgactgca cctgcgtaca
    36121 tgacaaggac gtgttgcaga ccgagtatga tcagctttcc tggtgataat atgcggtcaa
    36181 cctcatcagt tgggcggtct tgcccagaag atgagagaag ttgggaatct atggcgctca
    36241 tcagtgtttc tcctttcgcc cgccagagta aaaagcaacc gtttacgtta atgaaactgt
    36301 ctaaacacat gctcgtggag tgccagtttt tgcgaggaag gggagttaga gacaggaatt
    36361 gcggaggcaa tcacataatc gtggttaaac cacgaaaatg agaagagaac ctgacaatgg
    36421 aattatctgt aattttctca tattgatatt tcattaatca ttctggcatg tcgtactctc
    36481 gcatttaatc gtttttatct ggatagcact cttttgatcg gcaatgttga aataatgaaa
    36541 gcggcataga ttgaggggca ataatttgaa ctcagtcgca aaattataat taaaacggtc
    36601 atgctaccta atatatgccc gtt
//
LOCUS       DAZPEB010000015        36553 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZPEB010000015 DAZPEB010000000
VERSION     DAZPEB010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36553
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.015"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            310..396
                     /gene="leuL"
                     /locus_tag="ACHURJ_000715"
     CDS             310..396
                     /gene="leuL"
                     /locus_tag="ACHURJ_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFR1279779.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            489..2060
                     /gene="leuA"
                     /locus_tag="ACHURJ_000716"
     CDS             489..2060
                     /gene="leuA"
                     /locus_tag="ACHURJ_000716"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFR1279780.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2060..3151
                     /gene="leuB"
                     /locus_tag="ACHURJ_000717"
     CDS             2060..3151
                     /gene="leuB"
                     /locus_tag="ACHURJ_000717"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFR1279781.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDITGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3154..4554
                     /gene="leuC"
                     /locus_tag="ACHURJ_000718"
     CDS             3154..4554
                     /gene="leuC"
                     /locus_tag="ACHURJ_000718"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFR1279782.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGQVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4565..5170
                     /gene="leuD"
                     /locus_tag="ACHURJ_000719"
     CDS             4565..5170
                     /gene="leuD"
                     /locus_tag="ACHURJ_000719"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFR1279783.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(5219..6301)
                     /locus_tag="ACHURJ_000720"
     CDS             complement(5219..6301)
                     /locus_tag="ACHURJ_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1279784.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNTIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNAPLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSIAAGIFTLSLVLIAFML
                     PSVARVELPSENALSMQGGWQDSNVRMLFVASTLMWTCNTMYIIDMPLWISSELGLPD
                     KLAGFLMGTAAGLEIPAMILAGYYVKRYGKRRMMVIAVAAGVLFYTGLIFFHSRMALM
                     TLQLFNAVFIGIVAGIGMLWFQDLMPGRAGAATTLFTNSISTGVILAGVIQGAIAQSW
                     GHFAVYWVIAVISVVALFLTAKVKDV"
     gene            complement(6403..6534)
                     /gene="sgrT"
                     /locus_tag="ACHURJ_000721"
     CDS             complement(6403..6534)
                     /gene="sgrT"
                     /locus_tag="ACHURJ_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFR1279785.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            6623..8278
                     /gene="sgrR"
                     /locus_tag="ACHURJ_000722"
     CDS             6623..8278
                     /gene="sgrR"
                     /locus_tag="ACHURJ_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFR1279786.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVNYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      8312..8411
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8442..9425
                     /gene="thiB"
                     /locus_tag="ACHURJ_000723"
     CDS             8442..9425
                     /gene="thiB"
                     /locus_tag="ACHURJ_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFR1279787.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISGWQRAVSR"
     gene            9401..11011
                     /gene="thiP"
                     /locus_tag="ACHURJ_000724"
     CDS             9401..11011
                     /gene="thiP"
                     /locus_tag="ACHURJ_000724"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFR1279788.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPDEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLQQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            10995..11693
                     /gene="thiQ"
                     /locus_tag="ACHURJ_000725"
     CDS             10995..11693
                     /gene="thiQ"
                     /locus_tag="ACHURJ_000725"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFR1279789.1"
                     /translation="MLKLTDITWLYQHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(11806..12570)
                     /locus_tag="ACHURJ_000726"
     CDS             complement(11806..12570)
                     /locus_tag="ACHURJ_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR1279790.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(12656..13534)
                     /gene="araC"
                     /locus_tag="ACHURJ_000727"
     CDS             complement(12656..13534)
                     /gene="araC"
                     /locus_tag="ACHURJ_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFR1279791.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            13873..15573
                     /gene="araB"
                     /locus_tag="ACHURJ_000728"
     CDS             13873..15573
                     /gene="araB"
                     /locus_tag="ACHURJ_000728"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFR1279792.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLNWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQTQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            15584..17086
                     /gene="araA"
                     /locus_tag="ACHURJ_000729"
     CDS             15584..17086
                     /gene="araA"
                     /locus_tag="ACHURJ_000729"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFR1279793.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17286..17981
                     /gene="araD"
                     /locus_tag="ACHURJ_000730"
     CDS             17286..17981
                     /gene="araD"
                     /locus_tag="ACHURJ_000730"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFR1279794.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQSGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMEIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            18056..20407
                     /gene="polB"
                     /locus_tag="ACHURJ_000731"
     CDS             18056..20407
                     /gene="polB"
                     /locus_tag="ACHURJ_000731"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFR1279795.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGSENGDASSLDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20571..23477
                     /gene="rapA"
                     /locus_tag="ACHURJ_000732"
     CDS             20571..23477
                     /gene="rapA"
                     /locus_tag="ACHURJ_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFR1279796.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHMPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLLDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23489..24148
                     /gene="rluA"
                     /locus_tag="ACHURJ_000733"
     CDS             23489..24148
                     /gene="rluA"
                     /locus_tag="ACHURJ_000733"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFR1279797.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(24265..25080)
                     /gene="djlA"
                     /locus_tag="ACHURJ_000734"
     CDS             complement(24265..25080)
                     /gene="djlA"
                     /locus_tag="ACHURJ_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFR1279798.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            25335..27689
                     /gene="lptD"
                     /locus_tag="ACHURJ_000735"
     CDS             25335..27689
                     /gene="lptD"
                     /locus_tag="ACHURJ_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFR1279799.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWQHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVEHFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            27742..29028
                     /gene="surA"
                     /locus_tag="ACHURJ_000736"
     CDS             27742..29028
                     /gene="surA"
                     /locus_tag="ACHURJ_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFR1279800.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            29028..30017
                     /gene="pdxA"
                     /locus_tag="ACHURJ_000737"
     CDS             29028..30017
                     /gene="pdxA"
                     /locus_tag="ACHURJ_000737"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFR1279801.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMILNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            30014..30835
                     /gene="rsmA"
                     /locus_tag="ACHURJ_000738"
     CDS             30014..30835
                     /gene="rsmA"
                     /locus_tag="ACHURJ_000738"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFR1279802.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            30838..31215
                     /gene="apaG"
                     /locus_tag="ACHURJ_000739"
     CDS             30838..31215
                     /gene="apaG"
                     /locus_tag="ACHURJ_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFR1279803.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            31222..32064
                     /gene="apaH"
                     /locus_tag="ACHURJ_000740"
     CDS             31222..32064
                     /gene="apaH"
                     /locus_tag="ACHURJ_000740"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFR1279804.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(32142..32621)
                     /gene="folA"
                     /locus_tag="ACHURJ_000741"
     CDS             complement(32142..32621)
                     /gene="folA"
                     /locus_tag="ACHURJ_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFR1279805.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(32813..34675)
                     /gene="kefC"
                     /locus_tag="ACHURJ_000742"
     CDS             complement(32813..34675)
                     /gene="kefC"
                     /locus_tag="ACHURJ_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFR1279806.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(34668..35198)
                     /gene="kefF"
                     /locus_tag="ACHURJ_000743"
     CDS             complement(34668..35198)
                     /gene="kefF"
                     /locus_tag="ACHURJ_000743"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFR1279807.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGKALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(35306..36553)
                     /locus_tag="ACHURJ_000744"
                     /pseudo
     CDS             complement(35306..36553)
                     /locus_tag="ACHURJ_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         8843 a        10043 c         9658 g         8009 t
ORIGIN      
        1 tcaaaaaaaa attcgcaaaa catataattc aatacaaatc atcaggatag gttttgcaac
       61 gcgtgcattt tgtccccttt ttcctcgttg attagatgca aaaatttatt ctgaaatatg
      121 tcaacctatg aaaagtgtcg gtcgttaaac agaaattaat atcgcttgct ttaaccaccg
      181 cagcacaatt agctaatttt acggatgcag aactcgcgct ggcgggacgt ttttattgcg
      241 tcagggttga catccgtttt tgtatccagt aactctaaaa gcatatcgca ttcatctgga
      301 gctgatttaa tgactcacat cgttcgcttt atcggtctac tactactaaa cgcatcttct
      361 ttgcgcggta gacgagtgag cggcatccag cattaagcca gcacgcagtc aaacaaaaaa
      421 cccgcgccat tgcgcgggtt tttttatgcc cgaagcgagg cgctctaaaa gagacaagga
      481 cccaaaccat gagccagcaa gtcattattt tcgataccac attgcgcgac ggtgaacagg
      541 cgttacaggc aagcttgagt gtgaaagaaa aactgcaaat tgcgctggcc cttgagcgta
      601 tgggtgttga cgtgatggaa gtcggtttcc ccgtctcttc gccgggcgat tttgaatcag
      661 tgcaaaccat cgcccgccag gttaaaaaca gccgcgtatg cgcgttagct cgctgcgtgg
      721 agaaagatat cgacgtggcg gccgaatccc tgaaagtcgc cgaagccttc cgtattcata
      781 cctttattgc cacttcgcca atgcacatcg ccaccaagct gcgcagcacg ctggatgaag
      841 tgatcgaacg cgctatctat atggtgaaac gcgcccgtaa ttacaccgat gatgttgaat
      901 tttcttgcga agatgccggg cgtaccccca ttgccgatct ggcgcgagtg gtcgaagcgg
      961 cgattaatgc cggtgccacc accatcaaca ttccggacac cgtgggctac accatgccgt
     1021 ttgagttcgc cggaatcatc agcggtctgt atgaacgcgt acctaacatc gacaaagcca
     1081 ttatttccgt acatacccac gacgatttag gcctggcggt cggcaactca ctggcggcgg
     1141 tacatgccgg tgcacgccag gtggaaggtg caatgaacgg gatcggcgag cgtgccggta
     1201 actgttcgct ggaagaagtc atcatggcga ttaaagttcg taaggatatt ctcaacgtcc
     1261 acaccgccat taatcaccag gagatatggc gcaccagcca gttagttagc cagatttgta
     1321 atatgccgat cccggcaaac aaagccattg ttggcagcgg cgcattcgcc cactcctccg
     1381 gtatccacca ggatggcgtg ctgaaaaacc gcgaaaacta cgaaatcatg acaccagaat
     1441 ctattggtct gaaccaaatc cagctgaatc tgacctctcg ttcggggcgt gcggcggtga
     1501 aacatcgcat ggatgagatg gggtataaag aaagtgaata taatttagac aatttgtacg
     1561 atgctttcct gaagctggcg gacaaaaaag gtcaggtgtt tgattacgat ctggaggcgc
     1621 tggccttcat cggtaagcag caagaagagc cggagcattt ccgtctggat tacttcagcg
     1681 tgcagtctgg ctctaacgat atcgccaccg ccgccgtcaa actggcctgt ggcgaagaag
     1741 tcaaagcaga agccgccaac ggtaacggtc cggtcgatgc cgtctatcag gcaattaacc
     1801 gcatcactga ctataacgtc gaactggtga aatacagcct gaccgccaaa ggtcacggta
     1861 aagatgcgct gggtcaggtg gatattgtcg ccaactacaa cggtcgccgc ttccacggcg
     1921 tcggcctggc caccgatatt gtcgagtcct ccgccaaagc catggtgcac gtactgaaca
     1981 atatctggcg tgccgcagaa gtcgaaaaag agttgcaacg caaagctcaa cacaacgaaa
     2041 acaacaagga aaccgtgtga tgtcgaagaa ttaccatatt gccgtattgc cgggggacgg
     2101 tattggtccg gaagtgatga cccaggcgct gaaagtgctg gatgccgtgc gcaaccgctt
     2161 tgcgatgcgc atcaccacca gccattacga tgtaggcggc gcagccattg ataaccacgg
     2221 gcaaccactg ccgcctgcga cggttgaagg ttgtgagcaa gccgatgccg tgctgtttgg
     2281 ctcggtaggc ggtccgaaat gggaacattt accaccagac cagcaaccag aacgcggcgc
     2341 gctgttgcct ttgcgtaagc acttcaaatt attcagcaac ctgcgtccgg caaaactgta
     2401 tcaggggctg gaagcattct gtccgctgcg tgctgacatt gccgctaacg gcttcgacat
     2461 cctgtgcgtg cgcgaactga ccggcggcat ctatttcggt cagccaaaag gccgcgaagg
     2521 tagcggacag tatgaaaaag cgtttgatac cgaggtgtat caccgttttg agatcgaacg
     2581 tatcgcccgc atcgcgtttg aatctgcccg caagcgtcgc cacaaagtca cctcaatcga
     2641 caaagccaac gtgctgcaat cctctatttt atggcgggag atcgttaacg agatcgccac
     2701 ggaatacccg gatgtcgaac tggcgcatat gtacatcgac aacgccacca tgcagctgat
     2761 taaagatcca tcacagtttg acgtcctgct gtgctccaac ctgtttggcg acattctgtc
     2821 tgacgagtgc gcaatgatca ctggctcgat ggggatgttg ccttccgcca gcctgaacga
     2881 gcaaggtttt ggtctgtatg aaccggcagg cggctcagca ccagatatca caggcaaaaa
     2941 catcgccaac ccgattgcgc aaattctgtc gctggcactg ctgctgcgct acagcctgga
     3001 tgccgatgat gcggcttgcg ccattgaacg cgccattaac cgcgcattag aagaaggcat
     3061 tcgcaccggg gatttagccc gtggcgctgc cgccgttagt accgatgaaa tgggcgatat
     3121 cattgcccgc tatgtggcag aaggggtgta atcatggcta aaacgttata cgaaaaatta
     3181 ttcgacgctc acgtagtgta cgaagccgaa aacgaaaccc cgctgttata tatcgaccgc
     3241 cacctggtgc atgaagtgac ctcaccgcag gcgttcgatg gtctgcgcgc ccacggtcgc
     3301 ccggtacgtc agccgggcaa aaccttcgcc actatggatc acaacgtctc tacccagacg
     3361 aaagacatta atgcctgtgg tgaaatggcg cgcatccaga tgcaggagct gattaaaaac
     3421 tgcaaagaat ttggcgtcga actgtatgac ctgaatcacc cgtatcaggg gatcgtccac
     3481 gtaatggggc cggaacaggg cgtcaccttg ccggggatga ccattgtctg cggcgactcg
     3541 cataccgcca cccacggcgc gtttggcgca ctggcctttg gtatcggcac ttccgaagtt
     3601 gaacacgtac tggcaacgca aaccctgaaa cagggccgcg caaaaaccat gaaaattgaa
     3661 gtccagggca aagccgcgcc gggcattacc gcaaaagata tcgtgctggc aattatcggt
     3721 aaaaccggta gcgcaggcgg caccgggcat gtggtggagt tttgcggcga agcaatccgt
     3781 gatttaagca tggaaggtcg tatgaccctg tgcaatatgg caatcgaaat gggcgcaaaa
     3841 gccggtctgg ttgcaccgga cgaaaccacc tttaactatg tcaaaggccg tttgcatgcg
     3901 ccgaaaggca aagatttcga cgacgccgtt gcctactgga aaaccctgca aaccgacgaa
     3961 ggcgcaactt tcgataccgt tgtcactctg caagcagaag aaatttcacc gcaggtcacc
     4021 tggggcacca atccaggcca ggtgatttcc gtgaacgaca atattcccga tccggcttcg
     4081 tttgccgatc cggttgaacg cgcgtcggca gaaaaagcgc tggcctatat ggggctgaaa
     4141 ccgggcattc cgctgaccga agtggctatc gacaaagtgt ttatcggttc ctgtaccaac
     4201 tcgcgcattg aagatttacg cgcggcagcg gagatcgcca aagggcgaaa agtcgcgcca
     4261 ggcgtgcagg cactggtggt tcccggctct ggccaggtaa aagcccaggc ggaagcggaa
     4321 ggtctggata aaatctttat tgaagccggt tttgaatggc gcttgcctgg ctgctcaatg
     4381 tgtctggcga tgaacaacga ccgtctgaat ccgggcgaac gttgtgcatc caccagcaac
     4441 cgtaactttg aaggccgcca ggggcgcggc gggcgcacgc atctggtcag cccggcaatg
     4501 gctgccgctg ctgctgtgac cggacatttc gccgacattc gcaacattaa ataaggagca
     4561 gaccatggca gagaaattta tcaaacacac aggcctggtg gttccgctgg atgccgccaa
     4621 tgtcgatacc gatgcaatca tcccgaaaca gtttttgcag aaagtgaccc gtacgggttt
     4681 tggcgcgcat ctgtttaacg actggcgttt tctcgatgaa aaaggccaac agccaaaccc
     4741 ggacttcgtg ctgaacttcc cgcagtatca gggcgcttcc attttgctgg cacgagaaaa
     4801 cttcggctgt ggctcctcgc gtgagcacgc gccctgggca ttgaccgact acggttttaa
     4861 agtggtgatt gcgccgagtt ttgctgacat cttctacggc aatagcttta acaaccagct
     4921 gctgccggtg aaattaagcg atgcagaagt ggacgaactg tttgcgctgg tgaaagccaa
     4981 tccggggatc catttcgacg tggatctgga agcgcaagag gtgaaagcgg gagagaaaac
     5041 ctatcgcttt accatcgatg ccttccgccg ccactgcatg atgaacggtc tggacagtat
     5101 tgggcttacc ttgcagcacg acgacgccat tgccgcttat gaagcaaaac aacctgcgtt
     5161 tatgaattaa tccccttgcc cggtcaaatg accgggcttt ccgctatcgt ccacgtcatc
     5221 aaacgtcttt aacctttgcg gttaaaaata atgcgacaac agaaataacc gcaattaccc
     5281 agtagacagc aaagtgcccc caactttgtg caattgctcc ctgaataacg ccagccagaa
     5341 ttaccccggt agaaatactg ttagtaaata aggtggtagc tgcccccgct cttccaggca
     5401 ttaaatcctg aaaccatagc atcccaatac ccgcaacaat gccgataaat acagcgttaa
     5461 aaagttgcag cgtcatcaac gccatacggc tatgaaagaa aatcaatccg gtgtaaaaca
     5521 gtactcctgc cgccactgct atgaccatca ttcgccgctt accataacgt ttgacatagt
     5581 agccagccag aatcattgct ggtatttcca gtccagctgc cgtccccatc aggaaacccg
     5641 ccagtttgtc tggcaatcct aactcgctac tgatccacaa cggcatatca ataatgtaca
     5701 tggtgttgca ggtccacatt aacgtcgagg cgacaaataa catccgtacg ttactatcct
     5761 gccagccacc ttgcattgat aaagcatttt ccgacggcag ttctacccgc gccacagacg
     5821 gaagcataaa tgcaatcaat accagactga gtgtgaatat cccggcggca atcgaaaaca
     5881 tcaccacttc tcgcgccgag ttatccgcat attcccgcgc cagagcaaat aactgtggca
     5941 ttgccgtatt ggccagagat gccagaagca caccacaggt gataagcgtc agataatgac
     6001 gattaaatgc aaacaatggc gcattgccga tagccatcaa acagcaaaat ataatcagtt
     6061 ttcgccgatc gccctgactg tcagaacgtt ttgccaacca gaggcttacg ccgatcccag
     6121 caatagtatt caccgtataa aagagtccga tccagaaagg ttgcgcgcca acctcacgac
     6181 tcagaaataa gctcaatgta ggagcctgta gcgccccggc cacccccatc ataaaagcga
     6241 ccagcataaa tgccgcgtaa acaccgttca tacggcgagc catcgtcatt atccagatca
     6301 tacgttccct ttttagcgcg gcgagaataa aaaaaaccag caggtataat ctgctggcgg
     6361 gtgattttac accaatactc agtcacacat gatgcaggca agtcaacttt cagaattgcg
     6421 gtcatcccac tgcatcagtc cttcaagcat ggttaatcgt tgtgggacgc ttaaccaacg
     6481 caaccagcac aacttcgctg tcgcggtaaa atagtgctga taaaactgac gcatggggca
     6541 cctccttgct tcatcgttag gaattattga cttaatatag ggaaaataaa attgctgtct
     6601 tttgcgcagg agttcccctt ttatgccatc tgctcgtctg caacaacagt tcatccgcct
     6661 gtggcaatgc tgcgagggta aatcgcagga cacaacgctc aacgaactgg cagcgttatt
     6721 gagctgctcg cgtcgtcata tgcgtaccct gctcaacacc atgcaggatc gcggctggct
     6781 gacgtgggaa gcggaagtcg ggcgcggtaa acgctcgcgt ctgacattcc tctataccgg
     6841 gctggcgctt cagcaacagc gggcggaaga cctgctggag caggatcgta tcgaccaact
     6901 agtgcagttg gttggcgaca aagcgactgt gcggcaaatg ctggtttctc atctgggccg
     6961 cagcttccgc caggggcggc acatcctgcg cgtgctctac tatcgtccgt tgcgtaatct
     7021 gctacctggc agcgcattgc gccgttccga aacccatatt gcccggcaaa tcttcagttc
     7081 gctaacgcgc ataaatgagg aaaatgggga actggaagca gacatcgccc accactggca
     7141 gcaaatatca ccgcttcact ggcgtttctt tttgcgtcca ggagtccatt ttcatcatgg
     7201 tcgtgaactg gaaatggacg acgtgatcgc ctctttaaaa cgaatcaata cgctgccgct
     7261 ctattcgcat attgcggaca ttgtgtcgcc gacgccctgg acgctggata tccatctcac
     7321 gcaaccggac cgctggttac cgttactgct ggggcaagtt ccggcgatga tcctgccgcg
     7381 cgaatgggaa accctcagta actttgccag ccatcccatc ggcaccggtc cgtatgcggt
     7441 gatccgcaac accaccaatc aactgaaaat tcaggcattc gatgacttct tcggttaccg
     7501 ggcattaatc gatgaagtta acgtctgggt tctgccggaa attgccgacg agccagctgg
     7561 aggtctgatg ctaaaaggtc cacagggtga ggaaaaagag attgaaagcc gcctggagga
     7621 aggttgctac tatttactat tcgacagccg cacccatcgc ggagcgaatc agcaagtcag
     7681 ggactgggta aactatgtgc tttctccaac taatctggtc tatttcgctg aggaacagta
     7741 ccagcaactg tggttcccgg cttatggact gctcccccgt tggcaccatg cccgcaccat
     7801 aaagagcgaa aaaccggctg gcctggaaag cctcaccctg accttttatc aggatcacag
     7861 tgagcatcgg gtgattgccg ggatcatgca gcagattctg gcaagtcacc aggtcacgct
     7921 ggaaatcaaa gagatcagct acgatcagtg gcatgaagga gagatcgaga gcgatatctg
     7981 gcttaatagc gccaacttta cgctgccgct ggatttttcg ctgttcgcgc acttgtgcga
     8041 ggtgccgctg ctccaacact gtattccgat cgactggcaa gccgacgccg cccgctggcg
     8101 caatggcgaa atgaacctgg cgaactggtg ccagcaactg gtcgccagta aagcaatggt
     8161 gccgcttatc caccactggc tgatcattca gggacaacgc agtatgcgcg gcctgcgtat
     8221 gaataccctc ggctggttcg attttaaatc agcgtggttt gcgccgccgg atccatgatt
     8281 gctggtagat aacaaaatca ctacactaac gccgttctca acggggtgcc acgcaaacgc
     8341 gtgcgctgag aaaatacccg tcgaacctga tccggataac gccggcgaag ggatttgagg
     8401 ctccttctca agtcctttgc cactcttttt tgaggtgcaa agtgttaaaa aaatgtcttc
     8461 ccctgctgtt gctgtgcaca gcgcccgttt tcgctaaacc cgttctgact gtttatacct
     8521 acgattcctt cgccgccgac tgggggcctg gtccaaagat caaaaaagct tttgaagccg
     8581 actgtaactg cgaactgaaa ctggtggcgc tggaagatgg cgtttcgctt ctcaaccgtc
     8641 tacggatgga aggcaaaaat agtaaagccg atgtggtgct ggggctggat aacaatctgt
     8701 tagacgccgc cagtaaaacc ggactatttg ccaaaagcgg tgtggcagcg gatgccgtta
     8761 acgttcccgg cggctggaat aatgacactt tcgtcccgtt tgattacggc tatttcgcct
     8821 tcgtctatga caagaacaaa ctgaaaaacc cgccgcaaag cctgaaagaa ttggttgaga
     8881 gcgatcaaaa ctggcgggtg atttatcagg atccgcgcac cagtacgccg gggctgggcc
     8941 tgctgttgtg gatgcaaaaa gtctatggcg atgacgcccc acaagcctgg caaaaactgg
     9001 cgaagaaaac cgtcacggtc accaaaggct ggagcgaagc ctacggcctg tttttaaaag
     9061 gtgaaagcga tctggtactg agttacacca cctctccggc ttatcacatt ctcgaagaga
     9121 aaaaagataa ctacgccgcc gcgaacttta gcgaaggtca ctatctgcag gtggaagtcg
     9181 ccgcccgcac cgctgccagc aagcagccgg agctggcgca aaaattcctc cagtttatgg
     9241 tttctccggc ttttcagaat gcgatcccaa ccggtaactg gatgtatccg gtggcaaatg
     9301 tcacgctgcc tgccgggttt gaacaattga ccaaaccggc caccacgctg gagttcacac
     9361 cagctgaagt ggcggcacaa cgtcaggcat ggattagcgg atggcaacgc gccgtcagcc
     9421 gttaattccc ggctggttaa ttccaggtgt aagcgccgcc acgctggtgg tagcggttgc
     9481 gctcgcggcg tttctcgccc tgtggtggaa cgcgccgcag ggtaactggg tggcagtctg
     9541 gcaggacagc tacctgtggc atgtggtgcg cttctccttc tggcaggcgt ttctctcggc
     9601 gctactctct gtcgtacccg cgatattcct cgcccgcgcc ctctatcgca ggcgctttcc
     9661 gggtcggctg gcgctgttgc gtctgtgcgc aatgaccttg atcctcccgg tgctggttgc
     9721 tgttttcggc attcttagcg tctatggtcg ccagggctgg ctggcatcgc tctgccaatc
     9781 gctcggtctg gagtggacct tttcgcccta cggcctgcaa ggtattttgc tggcgcacgt
     9841 attttttaat ctgccgatgg cgagccgctt attactccag gcactggaaa acattcccga
     9901 cgaacaacgt cagcttgccg cccagcttgg gatgcgcggc tggcattttt tccgcttcgt
     9961 cgaatggccg tggttacggc gacaaatccc gccggttgct gcacttatct ttatgctctg
    10021 tttcgccagc ttcgccaccg tgctatcact gggcggcggt ccgcaggcga ccactatcga
    10081 gctggctatt tatcaggcgc tgagttacga ctacgatcct gcccgcgcgg cgatgctggc
    10141 gctgatccag atggtgtgtt gccttgggct ggtactgttg agtcagcgat tgagtaaggc
    10201 cattgcgcca ggcaccacgc tgctgcaagg ctggcgcgac ccggacgatc gtctgcatag
    10261 ccgcatttgc gacacggtgt taatagtgct ggcgttgctg ctgttgttgc caccgttgct
    10321 ggcggtgatc gtcgatgggg taaatcgcca gttgccggaa gtgctggcac aaccggtgct
    10381 gtggcaggcg ctgtggacct cgttgcgtat tgcgctggcg gcaggtgtat tgtgcgtagt
    10441 gctgaccatg atgctgctat ggagcagtcg cgaacttcgg gcacggcaga aaatgctggc
    10501 gggtcaggcg ctggagatga gcggcatgtt gattctcgcc atgccgggga ttgtgctggc
    10561 taccggcttc tttttactgc tcaacaacac tatcggcctg caacaatctg ctgacggcat
    10621 tgtgattttc accaatgcgt taatggcgat cccttatgcg ctgaaagtac tggaaaaccc
    10681 gatgcgcgat atcaccgccc gctacagtat gttgtgtcag tcgctgggca ttgaaggctg
    10741 gtcgcgctta aaagtggtgg agctgcgcgc cctgaaacgt ccactggcgc aggcgctggc
    10801 ctttgcctgc gtgctgtcga ttggtgattt tggcgtggtg gcgttgttcg gtaacgatga
    10861 tttccgtacc ctgccgtttt atctctacca gcaaattggc tcctatcgca gccaggacgg
    10921 cgcggtcacc gcgttaattc tgctgctact ctgttttctg ctgtttaccg tgattgaaaa
    10981 actaccgggg cgaaatgtta aaactgactg atatcacctg gctttaccaa catttgccga
    11041 tgcgttttag cttaacggtg gaacgcggcg agcaggtggc gatcctcggg ccaagcggcg
    11101 caggtaaaag taccctgctg aatttgattg ccggttttct gacgccagcc agcggttcgc
    11161 tgactatcga tggcgttgat cacaccacga cgccgccgtc acgccgtccg gtgtcgatgc
    11221 tgtttcagga gaacaacctg ttcagccacc tgacggtcgc gcagaacatc gggctggggc
    11281 ttaatccagg attgaagctg aacgcggcac agcaggagaa aatgcacgct atcgcccgtc
    11341 agatggggat tgataattta atggcgcggt taccgggcga gctttccggc ggccagcgac
    11401 agcgcgtggc gttagcgcgt tgtctggtgc gcgaacagcc gattttattg ctcgatgaac
    11461 cgttctctgc gctcgatccg gcgttacgtc aggagatgct gacgctggtg agcacgagct
    11521 gccagcagca aaaaatgacg ctattgatgg tgtcgcacag tgtggaagat gcggcgcgga
    11581 tcgccacacg ctcggtggtg gtcgccgacg ggcgcatcgc ctggcagggt aagactgaag
    11641 aattgttgag cggtaaggcg agtgcttcgg caatattggg gattacgggt tagtatgcac
    11701 tgtcagatgc ggcgtgaaca ccttatccga cctacggaca gtgcgttttg taggcctgat
    11761 aagaccggtc agcgtcgcat caggcaaccc gcacaagaca atcccctaaa ccccaaccac
    11821 tttacgcaaa atatcgatat acaccggcat caacgggtgg cgaattaaca ccaccagcgc
    11881 caccacgccg atggcagaaa tcaacggcgt cagccacaac aaacggccgc gcgacaaata
    11941 atgacttaaa cgatcggttg ctttaccgct gcgccataac cgccagcaca gccagccacc
    12001 aacccacaaa aataccgccg tcgccagcag caaccattta aactcgccgc tctgcattcc
    12061 ggctggaata tcgatcgccg cgcccgccag aatcccaggc aggaagtaaa acggtggcca
    12121 cagcaggcag ccgataatat tcggcgtaat gaattttgcc accggcagat ccagcattcc
    12181 cgccaccatt ggcaccagcg gacgcgtcgg gccaacaaag cgaccgacca gaatggtgaa
    12241 catgctgtgt tgatgcaacg catgttcagt tttatcgagt aatgctttgt ttttcttcag
    12301 aaatgaccag cgatgcaacg gctttttaaa acgccagccc agccagaagg agatccagtc
    12361 gcccagcaga caaccgacaa tccctgccag ccaggcgtgc cagaaactta actcgccgct
    12421 gccaatcagc gctcccagcc ccgccatcag caccgtaccg ggtagaatca aaccgaccag
    12481 cgccagcgac tccagaaagg ccaccaacac caccgccatc aatgaataca cggtagattg
    12541 ggtaataaag tgttccagca atgcttgcat aatgtgcctg tcaaatggac gaagcaggga
    12601 ttctgcaaac cctatgctac tccgtcaagc cgtcaattgt ctgattcgtt accaattatg
    12661 acaacttgac ggctacatca ttcacttttt cttcacaacc ggcacggaac tcgctcgggc
    12721 tggccccggt gcatttttta aatacccgcg agaaatagag ttgatcgtca aaaccaacat
    12781 tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa aagcagcttc gcctggctga
    12841 tacgttggtc ctcgcgccag cttaagacgc taatccctaa ctgctggcgg aaaagatgtg
    12901 acagacgcga cggcgacaag caaacatgct gtgcgacgct ggcgatatca aaattgctgt
    12961 ctgccaggtg atcgctgatg tactgacaag cctcgcgtac ccgattatcc atcggtggat
    13021 ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg
    13081 ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt
    13141 cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa acccgtattg gcaaatattg
    13201 acggccagtt aagccattca tgccagtagg cgcgcggacg aaagtaaacc cactggtgat
    13261 accattcgcg agcctccgga tgacgaccgt agtgatgaat ctctcctggc gggaacagca
    13321 aaatatcacc cggtcggcaa acaaattctc gtccctgatt tttcaccacc ccctgaccgc
    13381 gaatggtgag attgagaata taacctttca ttcccagcgg tcggtcgata aaaaaatcga
    13441 gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag atgggcatta aacgagtatc
    13501 ccggcagcag gggatcattt tgcgcttcag ccatactttt catactcccg ccattcagag
    13561 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
    13621 tctcgctaac ccaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
    13681 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
    13741 attatttgca cggcgtcaca ctttgctatt ccatagcatt tttatccata agattagcgg
    13801 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttgga
    13861 tggagtgaaa cgatggcgat tgcaattggc ctcgattttg gcagtgattc tgtgcgagct
    13921 ttggcggtgg actgcgccag cggtgaagag atcgccacca gcgtagagtg gtatccccgt
    13981 tggcaaaaag ggcaattttg tgatgccccg aataaccagt tccgtcatca tccgcgtgac
    14041 tacattgagt caatggaagc ggcactgaaa accgtgcttg cagagcttag cgtcgaacag
    14101 cgcgcagctg tggtcgggat tggcgttgac agtaccggct cgacgcccgc accgattgat
    14161 gccgacggta acgtgctggc gctgcgcccg gagtttgccg aaaacccaaa cgcgatgttc
    14221 gtattgtgga aagaccacac tgcggttgaa gaagcggaag agattacccg tttgtgccac
    14281 gcgccgggca acgttgacta ctcccgctac attggtggta tttattccag cgaatggttc
    14341 tgggcaaaaa tcctgcatgt gactcgccag gacagcgccg tggcgcaatc tgccgcatcg
    14401 tggattgagc tgtgcgactg ggtgccagct ctgctttccg gtaccacccg cccgcaggat
    14461 attcgtcgcg gacgttgcag cgccgggcat aaatctctgt ggcacgaaag ctggggcggc
    14521 ttgccgccag ccagtttctt tgatgagctg gacccgatcc tcaatcgcca tttgccttcc
    14581 ccgctgttca ctgacacctg gactgccgat attccggtgg gcaccttatg cccggaatgg
    14641 gcgcagcgtc tcggcctgcc tgaaagcgtg gtgatttccg gcggcgcgtt tgactgccat
    14701 atgggcgcag ttggcgcagg cgcacagcct aacgcactgg taaaagttat cggtacttcc
    14761 acctgcgaca ttctgattgc cgacaaacag agcgttggcg agcgggcagt taaaggtatt
    14821 tgcggtcagg ttgatggcag cgtggtacct ggatttatcg gtctggaagc aggccaatcg
    14881 gcgtttggtg atatctacgc ctggtttggt cgcgtactcg gctggccgct ggaacagctt
    14941 gccgcccagc atccggaact gaaagcgcaa atcaacgcca gccagaaaca actgcttccg
    15001 gcgctgaccg aagcatgggc caaaaatccg tctctggatc acctgccggt ggtgctcaac
    15061 tggtttaacg gccgccgcac accgaacgct aaccaacgcc tgaaaggggt gattaccgat
    15121 cttaacctcg ctaccgacgc tccgctgctg tttggcggtt tgattgctgc caccgccttt
    15181 ggcgcacgcg caatcatgga gtgctttacc gatcagggga tcgccgtcaa taacgtgatg
    15241 gcgctgggcg gcatcgcgcg gaaaaaccag gtcattatgc aggcctgctg cgacgtgctg
    15301 aatcgcccgc tgcaaattgt tgcctctgac cagtgctgtg cgctcggtgc ggcgattttt
    15361 gctgccgtcg ccgcgaaagt gcacgcagac atcccatcag cccagcaaaa aatggccagt
    15421 gcggtagaga aaaccctgca accgcgcagc gaacagacac aacgctttga acagctttat
    15481 cgccgctatc agcaatgggc gatgagcgcc gaacaacact atcttccaac ttccgccccg
    15541 gcacaggctg cccaggccgt tccgactcta taaggacacg ataatgacga tttttgataa
    15601 ttatgaagtg tggtttgtca ttggcagcca gcatctgtat ggcccggaaa ccctgcgtca
    15661 ggtcacccaa catgccgagc acgttgttaa tgcgctgaat acggaagcga aactgccctg
    15721 caaactggtg ttgaaaccgc tgggcaccac gccggatgaa atcaccgcta tttgccgcga
    15781 cgcgaattac gacgatcgtt gcgctggtct ggtggtgtgg ctgcacacct tctccccggc
    15841 caaaatgtgg atcaacggcc tgaccatgct caacaaaccg ttgctgcaat tccacaccca
    15901 gtttaacgcg gcgctgccgt gggacagtat cgatatggac tttatgaacc tgaaccagac
    15961 tgcacatggc ggtcgcgagt tcggcttcat tggcgcgcgt atgcgtcagc aacatgccgt
    16021 ggttaccggt cactggcagg ataaacaagc ccatgagcgt atcggctcct ggatgcgtca
    16081 ggcggtctct aaacaggata cccgtcatct gaaagtctgc cgttttggcg ataacatgcg
    16141 tgaagtagcg gtcaccgatg gtgataaagt tgccgcacag atcaagttcg gtttctccgt
    16201 caatacctgg gcggttggcg atctggtgca ggtggtgaac tccatcagcg acggcgatgt
    16261 taacgcgctg gtcgatgagt acgaaagctg ctacaccatg acgcctgcca cacaaatcca
    16321 cggcgaaaaa cgacagaacg tgctggaagc ggcgcgtatt gagctgggga tgaagcgttt
    16381 cctggaacaa ggtggcttcc acgcgttcac caccaccttt gaagatttgc acggtctgaa
    16441 acagcttcca ggtctggccg tacagcgtct gatgcagcag ggttacggct ttgcgggcga
    16501 aggcgactgg aaaaccgccg ccctgcttcg catcatgaag gtgatgtcaa ccggtctgca
    16561 gggcggcacc tcctttatgg aggactacac ctatcacttc gagaaaggta atgacctggt
    16621 gctcggctcc catatgctgg aagtctgccc gtcgattgcc gtagaagaga aaccgatcct
    16681 cgacgttcag catctcggta ttggtggtaa ggacgatcct gcccgactga tcttcaatac
    16741 ccaaaccggt ccagcgattg tcgccagcct gattgatctc ggcgatcgtt accgtctgct
    16801 ggttaactgt atcgacacgg tgaaaacacc gcactccctg ccgaaactgc cggtggcgaa
    16861 tgcgctgtgg aaagcgcaac cggatctgcc aactgcttcc gaagcgtgga tcctcgctgg
    16921 tggcgcgcac cataccgtct tcagccatgc gctgaacctc aacgatatgc gtcagttcgc
    16981 cgagatgcac gacattgaaa tcacggtgat tgataacgac acccgcctgc cagcgtttaa
    17041 agacgcactg cgctggaacg aagtgtatta cggatttcgt cgctaagtag ccacatcagg
    17101 tatgtaacgc ctgatgcgac gctgacgcgt cttatctggc ctacacgctg cgattttgta
    17161 ggccggataa gcaaagcgca tccggcattc aacgcctgat gcgacgctgg cgcgtcttat
    17221 caggcctacg cgctgcgatt ttgtagaccg gataagcgaa gcgcatccgg cacgaaggag
    17281 tcaacatgtt agaagatctc aaacgccagg tattagaagc caacctggcg ctgccaaaac
    17341 acaacctggt cacgctcaca tggggcaacg tcagcgccgt tgatcgcgag cgcggcgtct
    17401 ttgtgatcaa accttccggc gtcgattaca gcgtcatgac cgctgacgat atggtcgtgg
    17461 ttagcatcga aacaggtgaa gtggttgaag gtacgaaaaa gccctcctcc gacacgccaa
    17521 ctcaccggct gctctatcag gcattcccct ccattggcgg cattgtgcat acgcactcgc
    17581 gccacgccac catctgggcg cagtcgggtc agtcgattcc agcaaccggc accacccacg
    17641 ccgactattt ctacggcacc attccctgca cccgcaaaat gaccgacgca gaaatcaacg
    17701 gcgaatatga gtgggaaacc ggtaacgtca tcgtagaaac ctttgaaaaa cagggtatcg
    17761 atgcagcgca aatgcccggc gttctggtcc attcccacgg cccgtttgca tggggcaaaa
    17821 atgccgaaga tgcggtgcat aacgccatcg tgctggaaga ggtcgcttat atggagatat
    17881 tctgccgtca gttagcgccg cagttaccgg atatgcagca aacgctgctg gataaacact
    17941 atctgcgtaa gcatggcgcg aaggcatatt acgggcagta atgactgtat aaaaccacag
    18001 ccaatcaaac gaaaccaggc tatactcaag cctggttttt tgatggattt tcagcgtggc
    18061 gcaggcaggt tttatcttaa cccgacactg gcgggacacc ccgcaaggga cagaagtctc
    18121 cttctggctg gcgacggaca acgggccgtt gcaggttacg cttgcaccgc aagagtccgt
    18181 ggcgtttatt cccgccgatc aggttccccg cgctcagcat attttgcagg gtgaacaagg
    18241 ctttcgcctg acaccgctgg cgttaaagga ttttcaccgc cagccggtgt atggccttta
    18301 ctgtcgcgcc catcgccaat tgatgaatta cgaaaagcgc ctgcgtgaag gtggcgttac
    18361 cgtctacgag gccgatgtgc gcccgccaga acgctatctg atggagcggt ttatcacctc
    18421 accggtgtgg gtcgagggtg atatgcacaa tggcactatc gttaatgccc gtctgaaacc
    18481 gcatcccgac tatcgtccgc cgctcaagtg ggtttctata gatattgaaa ccacccgcca
    18541 cggtgagctg tactgcatcg gcctggaagg ctgcgggcag cgcatcgttt atatgctggg
    18601 gtcggagaat ggcgacgcct cctcgcttga tttcgaactg gaatacgtcg ccagccgccc
    18661 gcagttgctg gaaaaactca acgcctggtt tgccaactac gatcctgatg tgatcatcgg
    18721 ttggaacgtg gtgcagttcg atctgcgaat gctgcaaaaa catgccgagc gttaccgtct
    18781 tccgctgcgt cttgggcgcg ataatagcga gctggagtgg cgcgagcacg gctttaaaaa
    18841 cggcgtcttt tttgcccagg ctaaaggtcg gctaattatc gacggtatcg aggcgctgaa
    18901 atccgcgttc tggaatttct cttcattctc gctggaaact gtcgctcagg agctattagg
    18961 cgaaggaaaa tctatcgata acccgtggga tcgaatggac gaaattgacc gccgtttcgc
    19021 cgaagataaa cctgcgctgg caacttataa cctgaaagat tgcgagctgg tgacgcagat
    19081 cttccacaaa actgaaatca tgccattttt actcgaacgg gcaacggtga acggcctgcc
    19141 ggtggaccga cacggcggtt cggtggcggc atttggtcat ctctattttc cgcgaatgca
    19201 tcgcgctggt tatgtcgcgc ctaatctcgg cgaagtgccg ccgcacgcca gccctggcgg
    19261 ctacgtgatg gattcacggc cagggcttta tgattcagtg ctggtgctgg actataaaag
    19321 cctgtacccg tcgatcatcc gcacctttct gattgatccc gtcgggctgg tggaaggcat
    19381 ggcgcagcct gatccagagc acagtaccga aggttttctc gatgcctggt tctcgcgaga
    19441 aaaacattgc ctgccggaga ttgtgactaa catctggcac gggcgcgatg aagccaaacg
    19501 ccagggtaac aaaccgctgt cgcaggcgct gaaaatcatc atgaatgcct tttatggcgt
    19561 gctcggcacc accgcctgcc gcttcttcga tccgcggctg gcatcgtcga tcaccatgcg
    19621 tggtcatcag atcatgcggc aaaccaaagc gttgattgaa gcacagggct acgacgttat
    19681 ctacggcgat accgactcaa cgtttgtctg gctgaaaggc gcacattcgg aagaagaagc
    19741 ggcgaaaatc ggtcgtgcac tggtgcagca cgttaacgcc tggtgggcgg aaacgctgca
    19801 aaaacaacgg ctgaccagcg cattagaact ggagtatgaa acccatttct gccgttttct
    19861 gatgccaacc attcgcggag ccgataccgg cagtaaaaag cgctacgccg ggctgattca
    19921 ggagggcgat aaacagcgga tggtgtttaa agggctggaa accgtgcgca ccgactggac
    19981 gccgctggcc cagcagtttc agcaggagct atacctgcgc atcttccgca acgagccata
    20041 tcaggaatat gtacgcgaaa ccatcgacaa actgatggcg ggtgaactgg atgcgcgact
    20101 ggtttaccgt aaacgccttc gccgtccgct gagcgagtat cagcgtaatg tgccgcctca
    20161 tgtacgcgcc gctcgccttg ccgatgaaga aaaccaaaag cgtggtcgcc ccttgcaata
    20221 tcagaatcgc ggcaccatta agtacgtatg gaccaccaac ggcccggagc cgctggacta
    20281 ccaacgttca ccactggatt acgaacacta tctgacccgc cagctacaac ccgtggcgga
    20341 gggaatactc ccttttattg aggataattt tgctacactt atgaccgggc aacttgggct
    20401 attttgagca aaaaaagagt tcgccagata ccattttgat gcgtgacgaa tgctttgcca
    20461 tccagtacca tagcgccctt tccattcctg gacctgaata acaccactac ctcataagca
    20521 cggtagcggg tggttattgc ctgcaattaa agatatagag ccgaacacat atgcctttta
    20581 cacttggtca acgctggatc agcgatacag aaagcgaatt gggacttgga accgttgtcg
    20641 cggtggatgc gcgaactgtc actttacttt tcccatctac tggtgaaaac cgtctgtacg
    20701 cacgcagtga ttcccccgtg acccgcgtga tgttcaaccc tggtgatacc attaccagcc
    20761 atgacggctg gcagatgcaa gtcgaagaag taaaagaaga aaatggcttg ctgacctata
    20821 tcggtactcg cctggatact gaagagtccg gcgtagccct gcgtgaagtt ttccttgata
    20881 gcaaactggt gttcagcaaa ccgcaggacc gcctgtttgc cgggcagatt gaccgtatgg
    20941 accgctttgc gctgcgttat cgcgcgcgta agtattccag cgaacagttc cgtatgccgt
    21001 acagcggcct gcgcggtcag cgtaccagcc tgatcccgca ccagctcaac atcgctcatg
    21061 atgttggccg ccgccacgcg ccgcgcgtcc tgctggctga cgaagtgggt ttagggaaaa
    21121 ccattgaagc cgggatgatc ctgcatcagc aactgctctc tggcgctgct gaacgtgtgc
    21181 tgattatcgt cccggaaacc ttacagcatc agtggctggt agaaatgctg cgccgtttca
    21241 acctgcgctt tgctctgttt gatgatgagc gttatgccga agctcagcac gatgcttaca
    21301 acccgttcga caccgaacag ctggtgattt gctcgctgga ttttgcccgt cgtagcaaac
    21361 agcgcctgga acatctctgt gaagccgaat gggacctgct ggtggtcgat gaagcgcatc
    21421 acctggtgtg gagcgaagat gcgccaagcc gtgaatatca ggccattgaa caactggcag
    21481 agcacatgcc gggcgttctg ctgctgaccg cgaccccgga acagctgggg atggaaagcc
    21541 acttcgcccg tctgcgtctg ctggacccga accgtttcca cgattttgcg cagttcgttg
    21601 aagagcagaa aaattatcgt ccggttgcgg acgccgttgc catgctgctg gcaggtaaca
    21661 aactgagcaa tgacgaactg aacatgctcg gcgagatgat cggcgagcag gatatcgagc
    21721 cgctgttgca ggcagcaaac agcgacagcg aagatgccca gagcgcccgt caggagctgg
    21781 tttcgatgct gatggatcgc cacggcacca gccgcgtgct gttccgtaac acgcgtaacg
    21841 gtgtgaaagg attcccgaaa cgcgagctgc acaccattaa gctgccgcta ccgacgcagt
    21901 atcagacggc tattaaagtc tccggcatta tgggcgcacg taaaagtgcg gaagatcgtg
    21961 ctcgcgatat gctctacccg gagcgtattt atcaggaatt tgaaggtgat aacgccacct
    22021 ggtggaactt cgatccgcgc gttgagtggc tgatgggcta cctgaccagc catcgctctc
    22081 agaaagtgct ggtgatctgc gccaaagctg ccactgcgct gcaactggag caggtactgc
    22141 gcgaacgtga aggtattcgc gctgcggtgt tccacgaagg tatgtcgatt atcgaacgtg
    22201 accgcgctgc cgcctggttt gccgaagaag acaccggcgc acaggtactg ctgtgctcag
    22261 aaatcggttc tgaaggacgt aacttccagt tcgccagcca catggtgatg tttgacctgc
    22321 cattcaaccc ggatctactg gagcagcgta ttggtcgtct ggatcgtatc ggccaggcgc
    22381 acgatattca gatccatgtg ccttatctgg agaaaaccgc tcagtcggtg ctggtgcgct
    22441 ggtatcacga aggtctggat gcatttgagc acacctgccc gaccggacgc actatttacg
    22501 atagcgtata caacgatctg attaactatc tggcttcacc ggatcaaacc gaaggctttg
    22561 acgatctgat caaaaactgc cgcgagcaac atgaagcgct gaaagcacag ctggaacagg
    22621 gtcgtgaccg cctgctggaa atccactcca acggtggcga aaaagcccag gcactggcag
    22681 aaagcattga agagcaggat gacgatacca acctgatcgc cttcgccatg aacctgctcg
    22741 atattatcgg tatcaatcag gacgatcgcg gcgacaacat gatcgtgctg acgccgtccg
    22801 atcatatgct ggtgccggac ttccctggcc tgtcggaaga tggcatcacc atcacctttg
    22861 atcgtgaagt ggcgctggcg cgtgaagatg cacagtttat tacctgggag catccgctga
    22921 tccgcaacgg tctggatctg atcctttctg gcgataccgg tagcagcacg atttcactgt
    22981 taaaaaacaa agcgttgccg gtaggtacgc tgttggtgga actgatttat gtggttgaag
    23041 cccaggctcc gaagcagttg cagctcaacc gcttcctgcc accgacgccg gtacgtatgc
    23101 tgctggataa aaacggcaac aacctggcgg cgcaggtaga gtttgaaacc tttaaccgcc
    23161 agcttaacgc ggttaaccgt cacaccggca gcaaactggt taacgccgtg cagcaggatg
    23221 ttcacgctat ccttcaactg ggtgaagcgc agatcgagaa atctgcccgt gcattgattg
    23281 atgcagcgcg taacgaagcc gacgaaaaac tgtctgccga gctgtctcgt ctggaagctc
    23341 tgcgtgcagt gaacccgaac attcgtgacg acgaactgac cgccattgag agcaaccgtc
    23401 agcaggtaat ggaaagcctg gatcaggcag gttggcgtct ggatgccctg cgtttgatcg
    23461 ttgtaacgca tcagtaacgg agccgaaaat ggggatggaa aactacaatc caccgcagga
    23521 accctggttg gttatcctgt atcaggatga ccacattatg gtggttaaca agccgagcgg
    23581 tttgttgtct gtgccggggc gtctggaaga gcacaaagac agcgtgatga cgcgcattca
    23641 gcgtgattat ccgcaggcag aatcggtgca tcgtctggat atggctacca gcggcgtgat
    23701 tgtagtggcg ctgaccaaag ccgcggagcg ggagttaaaa cgccagttcc gcgagcgcga
    23761 gccgaaaaag cagtatgtgg cccgcgtctg ggggcatcca tcccctgcgg aaggtctggt
    23821 ggatctgccg ctgatttgcg actggccaaa ccgcccgaaa cagaaagtct gttacgaaac
    23881 gggtaaacct gcgcagacgg aatatgaagt ggtggagtat gcggcggata acacggcaag
    23941 agtggtgtta aaaccgatta ctgggcgttc gcatcaattg cgtgtgcata tgctggcgct
    24001 ggggcatccg attctcggcg atcgttttta tgcatcacca gaagcgagag cgatggcacc
    24061 gcgtttgttg ctacatgcag agatgttgac gattacccat ccggcgtatg gcaatagtat
    24121 gacgtttaaa gcgccagcgg atttttaata gtcacgctgg cgcagttcgg taggcggata
    24181 aggcgttcac gccgaatccg gcaattgtgc gccgattgcc tgatgcgacg cgggccgcgt
    24241 cttatcaggc ctacagttta aaacttattt aaaccctttc tgctgcttta tcagctcata
    24301 tgcctgctga atttcctgcg ctttctgctt cgccatctcc atcatctcag gcggcaaacc
    24361 tttcgccacc agcttatcgg gatggtgttc actcatcagc ttacggtagg ctcgtttaat
    24421 ggtggtcgca tcatccgtcg gcttcacacc cagcacatta caggcatctt ccagcgtcgg
    24481 gccacgctgc gcttgctgcc agttaccacc gccagattgc tgctgataac cgccgccaaa
    24541 ctgtgcaccg ccctgcatca tgcgcaaaaa ctggtcaaac tgggcgcggg agatccctaa
    24601 ttcttctgca atgacataca gcaccgcccg ttcattcggg tgcagtgaac catcagcaaa
    24661 cgccgcctga atctggatct ccagaaacat acgaattaag tcaaaacgac caaagcagac
    24721 actgcgaaac tggcgcatct tttcgcgcag cgggtaattg tctgattttc ccacccggaa
    24781 cgcgttttgc gccgcagtac gggaagcgcc atgaaggttc attcggtcca tcagctggct
    24841 ggcgatatga atatcagcct ccgtgacgcg acctttggat ttggttaaat gccccatcac
    24901 ttcaaaagtg gtggcaaaaa acagcgcctg acgctcacgc tggttggcga accacgccat
    24961 tttacggcta cgggctttat caaacatatg gccaattaac aggcctaaca ctacgcccca
    25021 aaagccgccg cccatcagta aggccacggc cacgccaatg atttttcccc aatactgcat
    25081 atattcccca aatcgacaca cggatatcag ggctatctcc cacaatataa aggtgctttt
    25141 accgttttcc ggctgcagtc aattgtggga catcgcctat aatttgcatt atcatacctg
    25201 tcattcaatg ccgtgcctaa caccactgac gctattcgga caggattaac actagcgtag
    25261 agatgacgag tacgttagtc tctgaccgtt tgtcacgcgc aacgttaccg atgatggaac
    25321 aataaaatca acgtatgaaa aaacgtatcc ccactctcct ggccaccatg attgccaccg
    25381 ccctttatag tcaacaggga ctggcagccg acctcgcctc acagtgcatg ttgggcgtgc
    25441 caagctatga ccgtcctctg gtacagggcg ataccaatga cttacccgtg actatcaatg
    25501 ctgaccacgc gaaaggggac tacccggatg acgccgtgtt tactggcagc gtggatatca
    25561 tgcagggtaa cagccgtctg caggccgacg aagtgcagct ccatcaaaaa gaggcaccag
    25621 gacaaccgga gccggtacga accgttgatg cgctcggtaa tgtccattac gacgataacc
    25681 aggtgatcct caaagggccg aaaggctggg cgaatctgaa caccaaagat accaacgtct
    25741 gggaaggtga ttaccagatg gtgggtcgcc agggccgcgg taaagcggac ctgatgaaac
    25801 aacgtggtga aaaccgctat accattctgg ataacggtag ctttacctcc tgtctgccgg
    25861 gttctgacac ctggagcgtg gtaggtagcg aaattattca tgaccgcgaa gaacaagttg
    25921 cggagatctg gaacgcccgc tttaaggtgg gtccagtacc gatcttttat agcccctatt
    25981 tgcagttgcc ggtgggtgac aaacgtcgct ctggtttctt gatcccgaac gccaagtaca
    26041 ccaccaccaa ctactttgag ttctacctgc catattactg gaacatcgcg ccaaatatgg
    26101 atgccaccat cacgccgcat tatatgcatc gtcgtggcaa catcatgtgg gagaacgaat
    26161 tccgctacct ctcccaggcg ggcgctggct tgatggaact ggactatctg ccttcagata
    26221 aagtctatga agatgaacac ccgaacgatg acagttcacg tcgttggttg ttctactggc
    26281 aacattccgg ggtcatggat caagtgtggc gtttcaacgt agactacacc aaggtcagcg
    26341 atcctagcta cttcaatgat ttcgataaca agtacggttc cagtactgac ggctacgcaa
    26401 cgcaaaaatt cagcgttggc tatgcggtgc aaaacttcaa tgccaccgtt tcaaccaagc
    26461 agttccaggt ctttagcgag cagaacacca gtagctactc ggcagagccg cagttagacg
    26521 ttaactacta ccagaatgat gttggtccgt ttgatacgcg tatttacggc caggcagtgc
    26581 actttgttaa caccagagac gacatgcctg aagcaacccg tgttcacctg gaaccgacca
    26641 tcaatttgcc gctctctaat aactggggca gcatcaatac cgaagcgaag ttgctggcaa
    26701 cccattatca gcaaaccaat cttgactggt ataactccag aaacacgacc aagctggacg
    26761 aatccgttaa ccgcgttatg ccgcaattca aagttgacgg caaaatggtc tttgaacgcg
    26821 atatggaaat gctggctccg ggttataccc aaacgctgga accgcgcgcg cagtatttgt
    26881 acgtgccgta tcgcgatcag agcgacatct ataactacga ctcgtctcta ctgcaatctg
    26941 actactctgg cctgttccgg gaccggactt acggcggtct tgaccgtatt gcctccgcta
    27001 accaggtgac gaccggtgtc acatctcgca tatatgatga tgctgccgtt gaacatttta
    27061 atatttccgt tggtcaaatc tactatttca cggagtctcg cactggcgat gacaacataa
    27121 catgggagaa tgacgacaaa acgggctcac tggtgtgggc aggcgatact tactggcgta
    27181 tctccgagcg ttggggattg cgtggcggga ttcagtacga tacacgtctg gataacgtag
    27241 cgaccagtaa ctccagcatt gaataccgtc gggatgaaga ccgtctggta cagctgaatt
    27301 accgttacgc cagcccggaa tatattcagg ctacgctgcc taagtactat tccactgctg
    27361 agcaatataa gaatggtatt tcgcaggtag gtgctgtcgc cagctggcca attgccgatc
    27421 gttggtccat tgttggggcc tactactacg acaccaatgc gaacaagcaa gccgactcta
    27481 tgttaggtgt gcaatacagc tcctgctgct atgcaattcg cgtcggttac gagcggaagc
    27541 tgaacggttg ggataacgat aaacaacatg cggtatatga caacgcaatc ggctttaaca
    27601 tcgaacttcg cggcctgagc tccaactacg gtctgggtac gcaagagatg ctgcgttcga
    27661 acattctgcc gtatcaaaac actttgtgat ttgttgattt accacgtaat ccgcagtgcg
    27721 gttaattgaa atggaaaaag tatgaagaac tggaaaacgc tgcttctcgg tatcgccatg
    27781 atcgcgaata ccagtttcgc tgccccccag gtagtcgata aagtcgcagc cgtcgtcaat
    27841 aacggcgtcg tgctggaaag cgacgttgat ggattaatgc agtcggtaaa actgaacgct
    27901 gcgcaggcaa ggcagcagct tcctgatgac gcgacgctgc gccaccaaat catggaacgt
    27961 ttgatcatgg atcaaatcat cctgcagatg gggcagaaaa tgggagtgaa aatctccgat
    28021 gagcagctgg atcaggcgat tgctaacatc gcgaaacaga acaacatgac gctggatcag
    28081 atgcgcagcc gtctggctta cgatggtctg aactacaaca cctatcgtaa ccagatccgc
    28141 aaagagatga ttatctctga agtgcgtaac aacgaggtgc gtcgtcgcat caccatcctg
    28201 ccgcaggaag tcgaatccct ggcgcagcag gtgggtaacc aaaacgacgc cagcactgag
    28261 ctgaacctga gccacatcct gatcccgctg ccggaaaacc cgacttctga tcaggtgaac
    28321 gaagcggaaa gccaggcgcg cgccattgtc gatcaggcgc gtaacggcgc tgatttcggt
    28381 aagctggcga ttgctcattc tgccgaccag caggcgctga acggcggcca gatgggctgg
    28441 ggccgtattc aggagttgcc gggtatcttc gcccaggcat taagcaccgc gaagaaaggc
    28501 gacattgttg gcccgattcg ttccggcgtt ggcttccata ttctgaaagt taacgacctg
    28561 cgcggcgaaa gcaaaaatat ctcggtgacc gaagttcatg ctcgccatat tctgctgaaa
    28621 ccgtcgccga tcatgactga cgaacaggcc cgtgtgaaac tggaacagat tgctgccgat
    28681 atcaagagtg gtaaaacgac ttttgctgcc gcagcgaaag agttctctca ggatccaggc
    28741 tctgctaacc agggcggtga tctcggctgg gctacaccag atattttcga tccggccttc
    28801 cgtgacgccc tgacccgcct gaacaaaggt caaatgagtg caccggttca ctcttcattc
    28861 ggctggcatt taatcgaact gctggatacc cgtaatgtcg ataaaaccga cgctgcgcag
    28921 aaggatcgtg cataccgcat gctgatgaac cgtaagttct cggaagaagc agcaagctgg
    28981 atgcaggaac aacgcgccag cgcctacgtt aaaatcctga gcaactaatg gttaaaaccc
    29041 aacgtgttgt gatcactccc ggcgagcccg ccgggattgg cccggactta gttgtccagc
    29101 ttgcacagcg tgagtggccg gtcgaactgg ttgtttgtgc cgatgccact ctccttaccg
    29161 accgggcagc gatgctcggt ttgccgctca ccctccgccc ttattccccc aactcccctg
    29221 cacaaccgca aactgcgggc acattaacgc tacttcctgt cgcgctacgc gaatctgtca
    29281 ctgcggggca gttagcgatt gaaaatgggc attacgtggt ggagacgctg gcgcgagcgt
    29341 gtgatggctg tctgaacggt gaatttgctg cgctgatcac aggccccgtg cataaaggcg
    29401 tcattaacga cgcaggtatt ccgtttactg gtcataccga gtttttcgaa gagcgctcgc
    29461 aggcgaaaaa agtggtgatg atgctggcga ccgaagaact tcgcgtggcg ctggcaacga
    29521 cgcatttacc gctgcgcgat atcgcagatg ctatcacccc tgcacttttg cacgaagtga
    29581 ttgctatttt gcatcacgat ttgcgtacaa aatttggtat tgccgaaccg cgcattctgg
    29641 tctgcgggct gaatccgcac gcgggcgaag gcggtcatat gggtacggaa gagatagaca
    29701 ccattattcc ggtgctcgac gagctgcggg cgcaggggat gatactcaac gggccgctgc
    29761 ctgccgatac cctgtttcag ccgaaatatc tcgataacgc cgacgccgtg ctggcgatgt
    29821 accacgatca gggtcttccc gtgctaaaat accagggctt cgggcgcggt gtgaacatta
    29881 cgctgggcct gccctttatt cgcacatcag tggaccacgg caccgcgctt gaactggcgg
    29941 gacgtggcga agccgatgtc ggcagtttta ttacggcgct taatctcgcc atcaaaatga
    30001 ttgttaacac ccaatgaata atcgagtcca ccagggccac ttagcccgta aacgcttcgg
    30061 gcaaaacttt ctcaacgatc agttcgtgat cgacagcatt gtttctgcca ttaacccgca
    30121 aaagggccag gcaatggtcg aaatcggccc cggtctggcg gcattgaccg agccggtcgg
    30181 cgaacgtctg gaccagctga cggtcatcga acttgaccgc gatctggcgg cacgtctgca
    30241 aacgcatcca ttcttaggcc cgaaactgac gatttatcag caggatgcga tgacctttaa
    30301 ctttggcgaa ctggccgaga aaatgggtca gccgctgcgt gttttcggca acctgcctta
    30361 taacatctcc acgccgttga tgttccatct gtttagctat actgatgcca ttgccgacat
    30421 gcactttatg ttgcaaaaag aggtggtgaa tcgtctggtt gcaggaccga acagcaaagc
    30481 gtatggtcga ttaagcgtca tggcgcaata ctattgcaac gtgatcccgg tactggaagt
    30541 tccgccgtca gcctttacac caccacccaa agtggattcc gccgtcgtgc gcctggttcc
    30601 tcatgcaacg atgcctcacc cggttaaaga tgttcgtgtg ttgagccgca tcaccaccga
    30661 agcctttaac cagcgtcgta aaaccattcg taacagcctc ggcaacctgt ttagcgtcga
    30721 ggtgttaacg ggaatgggga tcgacccggc gatgcgagcg gaaaatatct ctgtcgcgca
    30781 atattgccag atggcgaact atctggcgga gaacgcgcct ttgcaggaga gttaacgatg
    30841 atcaattcgc cccgagtgtg tattcaggtt caaagcgtct acattgaggc acaatcttca
    30901 cctgataatg aacgttacgt ttttgcttat accgtaacca tacgcaatct ggggcgagcg
    30961 ccagtgcagt tgttggggcg ttactggctg atcaccaatg gcaatggccg tgaaaccgaa
    31021 gtccagggcg aaggagtggt tggcgtccag ccacttatcg cgcctggcga agagtaccag
    31081 tacaccagcg gtgcaatcat tgaaaccccg ctgggcacca tgcagggtca ctacgaaatg
    31141 atcgatgaaa atggcgtccc tttcagcatc gacattcccg tattccgact cgccgttccc
    31201 acactcattc attaaaagaa tatggcgaca taccttattg gcgacgttca tggttgttac
    31261 gatgaactga tcgcattgct gcataaagta gaatttaccc ctgggaaaga taccctctgg
    31321 ctgacgggcg atctggtcgc gcgcggcccg ggttcgctgg atgttctgcg ctatgtgaaa
    31381 tccttaggcg acagcgtacg tctggtgctg ggcaatcacg atctgcatct gctggcggta
    31441 tttgccggga tcagccgcaa taaaccgaaa gatcgcctga caccgctgct ggaagcgccg
    31501 gatgccgacg agctgcttaa ctggctgcgt cgccagcctt tgctgcaaat cgacgaagag
    31561 aaaaagttgg tgatggccca cgcagggatc acgccgcagt gggatctaca gaccgccaaa
    31621 gagtgcgcgc gcgatgtaga agcggtgcta tcgagtgact cctatccctt ctttctcgac
    31681 gctatgtacg gcgatatgcc aaataactgg tcaccggaat tgcgggggct gggaagactg
    31741 cgttttatca ccaacgcctt tacccgtatg cgtttttgct tcccgaacgg tcaactggat
    31801 atgtacagca aagaatcgcc ggaagaggcc cctgccccac tgaaaccgtg gtttgcgatt
    31861 cctggccctg tcgctgaaga atacagcatc gcctttggtc actgggcatc gctggagggc
    31921 aaaggtacgc cggaaggtat atacgcgctg gataccggct gctgctgggg tggtacatta
    31981 acctgcctgc gctgggaaga taaacagtat tttgtccagc cgtcgaaccg gcataaggat
    32041 ttgggcgaag cggcggcgtc ttaaacacag cctgatatag gaaggccgga taagacgcga
    32101 ccggcgtcgc atccggcact agccgtaaat tctatacaaa attaccgccg ctccagaatc
    32161 tcaaagcaat agctgtgaga gttctgcgca tcagcatcgt ggaattcgct gaataccgat
    32221 tcccagtcat ccggctcgta atccgggaaa tgggtgtcgc cttccacttc tgcgtcgata
    32281 tgcgtcagat acagtttctg cgcttttggc aggaactgct cataaacgcg accgccgcca
    32341 atcaccatga tttctggtac gtcaccacac gccgcgatgg cttcatccac cgacttcacc
    32401 cacgttacgc gatcgtccgt acccggttga ctgctgagga taatattttt gcgtcctggc
    32461 aacggacgac cgattgattc ccaggtatgg cggcccataa tcacgggttt atttaaggtg
    32521 ttgcgtttaa accaggcgag atcggcaggc aggttccacg gcatggcgtt ttccatgccg
    32581 ataacgcgat ctaccgctaa cgccgcaatc agactgatca ttgagatttc ccgataaaaa
    32641 aaattgtcgc cactatacgt aaagcgtaaa ccgtcgtcga ctggtgcgag gatgatgttg
    32701 aggaaaattt tatattctgc tggcgagtcc acgctctctc cctggactcg ccgcattaca
    32761 atgaaacaaa aacaaacagt tagctgtaaa gtgtgattta tgtcactctt tattaggatg
    32821 agggtttcgt ttccggttca tccgccatgt tgccggtatg tttaccttct tcggttccct
    32881 gccagccatg tcgttgaatt aatgacagat gttcgcggtc ctcggtaatg atctcactta
    32941 acatcgcgct ggtgcgttta tagaccgccg cgcgggcttt ggtgtcgttc tcaaccattg
    33001 ccatctcttc caccatctga atattaaagc ggcggaacac atcggcacgt tctcgcgctt
    33061 catacggccc cagacctaaa ctttccagtg ccagacgccc ggttttcagc gcaccttcga
    33121 aggtttcacg ctccggcttt tcaacgcctg cctgacgcaa acgaatgtag tggtcgacat
    33181 cgcgggcgcg ggcaataatc tgcaaatgcg ggaagtgttc tttcaccatc tctgtcagtt
    33241 gcaggttggt ttgcggatcg tcgatggcgt taatcagcac ttccgctttc gccgctcccg
    33301 cagattccag taaatccatc cgcgtggcat cgccataaaa cactttcata ccaaatttac
    33361 gcaaggtttc gatatggtcc ggatcgtgat cgagcaccac cattttcacc ccgctggaga
    33421 gcagtaaacg tccggtgatc tgcccaaaac gaccgaatcc ggcaatgatt acccgcggct
    33481 gttcttcgtc gatctcatcg gcttcacgcg cctcttcagt agaagattgc tcaaggcgat
    33541 tgaggatcac cagcagaatc ggcgttgccg ccatcgacag cgccaccgcc agggtcagcg
    33601 atttcgccca ctccggctcc agcacattcg ccatctgcgc cgcgccaaat accacaaagg
    33661 caaactcact gccctgccct aacaacaccg caaaccaacg acgctgttta tttggcactt
    33721 gcaacggtcg ggcaatcagc cacagcatgg cgattttgat gatgaggaaa ccgagcagca
    33781 aaatgacaat gcgcaatggg ttttcaagca gcgtgccaaa gtctatcgac atgccaacac
    33841 cgatgaaaaa cagccccaac agcaaacctt taaagggttc gatatcgctc tccagcgcat
    33901 gacggtattc cgagcttgcc agcagtacgc ccgccagaaa cgcgcccatc gccatcgaca
    33961 agccgacctc ttccagcagc aaaccaaagc caaacacgag gaataacgcc acggcactaa
    34021 acacttcccg caagccagag cgggctacaa aacgcagcgc cggacgcgtg acatagcgcc
    34081 ccagcaatac caccagcacc agcgcacccg ccacttttaa cgccgagaga gcaaatgcgc
    34141 ccatcgtcgt cgaggcactg ctcgttgcca gtagcggaat catcgccacc agcgggatcg
    34201 ccgcgatatc ctggaacagc agcaccgcaa aggcactgcg acccatttgc gtcaccatca
    34261 gattgcgttc attcatcgcc tgcatggcaa tcgccgtaga ggagagcgcc agcgtcatgc
    34321 cgatcaattc cgcgacctgc cagcgcagcc caagtaacat gcagaacagc cccagcaggc
    34381 cgccgcaaat caccatctgc aatgcgccac cgccgaacac tgccgcacgc agcttccaca
    34441 gcctttgtgg atcgagttcg aggccgataa taaacagcat cagcaccacc ccaatctcgg
    34501 caaagtgcag aatagattcg gcatcggtca ccagtcgcag cccccacggg ccaataatgc
    34561 agccggcgat caggtagcca agtaccgatc ccagaccaag acgtaccgca atgggtacaa
    34621 tcagcgctgc cgaaccgaga taaatcagcg cctgaatcag cgtatggcta tccatgatgg
    34681 gcctcctgcc attccagcag acgttgctta tagtgacgcg cctgcccttc gagggtttcg
    34741 tcgtcacaaa taaaggtgca gtgcatggca aacggtggca gccagttcag cccgcagtag
    34801 attgccgtcg cctgtagcgg ctgcgacagc acatcaaagc ccggatgcgc accaatttca
    34861 aaatggcttt ccccgccgcc ggtcgtcacc gcccacagca aatgtttgcc atgcagcgcc
    34921 ttgccgccgt gaccgtaagc ccagccgtgc gacaaaactt tatcgatcca aagtttaaga
    34981 agcggaggaa tgctgtacca ctgcatcgga tgctgccaga cgatcagatc ggcgcgagac
    35041 agcgcctcct gctcggcggc aatatcgata ttgaagtcag gatagagttg ataaagagag
    35101 cgaatttcga cgccttccag cgtccttgcc tgttcaagca tccgtttatt cgcatgggaa
    35161 tgatgcggat acggatgcgc ataaattata agaatcatag gtagcccgcg tgattacgcc
    35221 tttgttttcc cacaaagtgt agtcatatat tcacgcggct tatagtcaat aatattgatt
    35281 cagaaaagag agaaatctga acaatttacc ccattctctg cccgcggatc gtcatattgc
    35341 tggtttgcgc caatgacatc cctcgatgtc tccggggcaa acgctacgga aatcaacaag
    35401 ccaaacagcg agatacccgc ccccattagc atcgtgttac tgataccgta attattgata
    35461 aagatcggca gtgcccaggt cgaaacaatg gtgccaatac ggcttaagga cataatcacg
    35521 cccacggcag aggcgcggat atctgtcggg aagagttcat taggatagag ccactgcaaa
    35581 ttacccggcc cgccagagaa aaaggcatac accgcaaagg ccatcactac cagccagatc
    35641 cccatatccg ggatcagccc caaaaccgcc agcgccagcg tcatcatggc aaagctgcca
    35701 atcaacaatg gacgccgtcc ggcagtgttt aaccacagca tcggcggaat acagccgagc
    35761 ataaagaaca ggctaatcac cacgttcccc agcgccgcgt ttttgccaac acccaatccc
    35821 aacaaaccaa cgatttgcgg gccaaaggtg taaatggcga acattgggat cacctggcag
    35881 gtccagatgg cggcaacaaa cagaacaaaa ggaaaatggc ggcgattaaa cagatcgcga
    35941 aaacgggttt gctgcggctg ctcttcatcg aaagccaccg gttcgccaaa cagttttatc
    36001 atcatctctt cgcactcttt tactcgccct ttgcgtaata accagcgggg agattcagga
    36061 agttcgaatc gaccaatcaa aatcaacaaa caggggatcg ccgcgctacc cagcatccag
    36121 cgccagccgc cttccacatc ataaagccag tagccgacca gatcggcgca ggtcgcgccg
    36181 acataccaca tggcggcgat aaagctgatg gaaaatgccc gctgacgggt actggagaac
    36241 tcggtgatca ttgaggtggc gatgggataa tcggcaccga tgacaatgcc gataagtacc
    36301 cgcatcacca acagttcgac gggggatgaa acaaacatcg tcgccaccga tatcacgccg
    36361 atggcgatga tatcaatgag gaacattttg cgccgtccga ctttatcgga aatatagcca
    36421 aacagcgatg tgccaacgaa cagcccggcg agcgttcccg cgcccaacaa gccaatccag
    36481 tcagcgtcca gttttagcgc cggagtcagt tgctccagcg ccacgccaat cattaccagt
    36541 acataaccat cca
//
LOCUS       DAZPEB010000016        36206 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZPEB010000016 DAZPEB010000000
VERSION     DAZPEB010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36206
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.016"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(50..1699)
                     /gene="actP"
                     /locus_tag="ACHURJ_000745"
     CDS             complement(50..1699)
                     /gene="actP"
                     /locus_tag="ACHURJ_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFR1279808.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGMLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGTMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1696..2010)
                     /locus_tag="ACHURJ_000746"
     CDS             complement(1696..2010)
                     /locus_tag="ACHURJ_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFR1279809.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2142..4101)
                     /gene="acs"
                     /locus_tag="ACHURJ_000747"
                     /pseudo
     CDS             complement(2142..4101)
                     /gene="acs"
                     /locus_tag="ACHURJ_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
     gene            4494..5930
                     /gene="nrfA"
                     /locus_tag="ACHURJ_000748"
     CDS             4494..5930
                     /gene="nrfA"
                     /locus_tag="ACHURJ_000748"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFR1279810.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            5975..6541
                     /gene="nrfB"
                     /locus_tag="ACHURJ_000749"
     CDS             5975..6541
                     /gene="nrfB"
                     /locus_tag="ACHURJ_000749"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFR1279811.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6538..7209
                     /gene="nrfC"
                     /locus_tag="ACHURJ_000750"
     CDS             6538..7209
                     /gene="nrfC"
                     /locus_tag="ACHURJ_000750"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFR1279812.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7206..8162
                     /gene="nrfD"
                     /locus_tag="ACHURJ_000751"
     CDS             7206..8162
                     /gene="nrfD"
                     /locus_tag="ACHURJ_000751"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFR1279813.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLAQQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKSWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8182..9900
                     /locus_tag="ACHURJ_000752"
     CDS             8182..9900
                     /locus_tag="ACHURJ_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFR1279814.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQCALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELMPERRFYEARRQ
                     QMMEPSIRWNGIHDWYVVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            9893..10276
                     /gene="nrfF"
                     /locus_tag="ACHURJ_000753"
     CDS             9893..10276
                     /gene="nrfF"
                     /locus_tag="ACHURJ_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFR1279815.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10273..10869
                     /gene="nrfG"
                     /locus_tag="ACHURJ_000754"
     CDS             10273..10869
                     /gene="nrfG"
                     /locus_tag="ACHURJ_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFR1279816.1"
                     /translation="MKEPKIPVKMLTTLTILMVFLCVGSYLLSPKWQAVHAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11210..12523
                     /gene="gltP"
                     /locus_tag="ACHURJ_000755"
     CDS             11210..12523
                     /gene="gltP"
                     /locus_tag="ACHURJ_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFR1279817.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12714..13402)
                     /gene="yjcO"
                     /locus_tag="ACHURJ_000756"
                     /pseudo
     CDS             complement(12714..13402)
                     /gene="yjcO"
                     /locus_tag="ACHURJ_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
     gene            complement(13496..15643)
                     /gene="fdhF"
                     /locus_tag="ACHURJ_000757"
     CDS             complement(13496..15643)
                     /gene="fdhF"
                     /locus_tag="ACHURJ_000757"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(15224..15226),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HFR1279818.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIMANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGEQGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGSCNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15841..17307)
                     /gene="mdtP"
                     /locus_tag="ACHURJ_000758"
     CDS             complement(15841..17307)
                     /gene="mdtP"
                     /locus_tag="ACHURJ_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFR1279819.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPSLGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDSIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIHSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17304..19355)
                     /gene="mdtO"
                     /locus_tag="ACHURJ_000759"
     CDS             complement(17304..19355)
                     /gene="mdtO"
                     /locus_tag="ACHURJ_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFR1279820.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPA
                     AKPPSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDLLHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSCSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19355..20386)
                     /gene="mdtN"
                     /locus_tag="ACHURJ_000760"
     CDS             complement(19355..20386)
                     /gene="mdtN"
                     /locus_tag="ACHURJ_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFR1279821.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDVLVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPNDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20405..20680)
                     /locus_tag="ACHURJ_000761"
     CDS             complement(20405..20680)
                     /locus_tag="ACHURJ_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFR1279822.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRIIQRANINLAFVGIIYTALFAVYAMLFWLAFF"
     gene            complement(20889..22874)
                     /gene="yjcS"
                     /locus_tag="ACHURJ_000762"
     CDS             complement(20889..22874)
                     /gene="yjcS"
                     /locus_tag="ACHURJ_000762"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HFR1279823.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSHRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVRGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVISLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQPQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
     gene            23127..23444
                     /locus_tag="ACHURJ_000763"
     CDS             23127..23444
                     /locus_tag="ACHURJ_000763"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HFR1279824.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
     gene            23513..23842
                     /gene="yjdP"
                     /locus_tag="ACHURJ_000764"
     CDS             23513..23842
                     /gene="yjdP"
                     /locus_tag="ACHURJ_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFR1279825.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(23989..24747)
                     /gene="phnP"
                     /locus_tag="ACHURJ_000765"
     CDS             complement(23989..24747)
                     /gene="phnP"
                     /locus_tag="ACHURJ_000765"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFR1279826.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECVACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGLHDLADRWPPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPREDAPRSHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEAGFDGMEIGVA"
     gene            complement(24749..25213)
                     /gene="phnO"
                     /locus_tag="ACHURJ_000766"
     CDS             complement(24749..25213)
                     /gene="phnO"
                     /locus_tag="ACHURJ_000766"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HFR1279827.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PSMRYPSMRYPSMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQACGLN
                     VGSKLLAWAEEEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            complement(25200..25757)
                     /gene="phnN"
                     /locus_tag="ACHURJ_000767"
     CDS             complement(25200..25757)
                     /gene="phnN"
                     /locus_tag="ACHURJ_000767"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFR1279828.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEREFFTRAGQNLLALSWHANGLYYGVGIEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLKNRGRENTSEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            complement(25757..26893)
                     /gene="phnM"
                     /locus_tag="ACHURJ_000768"
     CDS             complement(25757..26893)
                     /gene="phnM"
                     /locus_tag="ACHURJ_000768"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFR1279829.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLNAVAIGDVR
                     DGGDQLENLEKMINTIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARQIALASHDDATHAHVAESHQLGSVIAEFTTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVRLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRQGNHIHIDHVWRQGKRVF"
     gene            complement(26890..27570)
                     /gene="phnL"
                     /locus_tag="ACHURJ_000769"
     CDS             complement(26890..27570)
                     /gene="phnL"
                     /locus_tag="ACHURJ_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFR1279830.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIHEAKARGAAIVGIFHDEAVRNDVADR
                     LHPMGASS"
     gene            complement(27681..28439)
                     /gene="phnK"
                     /locus_tag="ACHURJ_000770"
     CDS             complement(27681..28439)
                     /gene="phnK"
                     /locus_tag="ACHURJ_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFR1279831.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYESRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            complement(28436..29281)
                     /gene="phnJ"
                     /locus_tag="ACHURJ_000771"
     CDS             complement(28436..29281)
                     /gene="phnJ"
                     /locus_tag="ACHURJ_000771"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFR1279832.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            complement(29274..30332)
                     /gene="phnI"
                     /locus_tag="ACHURJ_000772"
     CDS             complement(29274..30332)
                     /gene="phnI"
                     /locus_tag="ACHURJ_000772"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFR1279833.1"
                     /translation="MYVAVKGGEKEIDAAHALQESRRRGNTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQSPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVSPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIMPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHYVD
                     FQAELELLKRLQQEQNHG"
     gene            complement(30332..30916)
                     /gene="phnH"
                     /locus_tag="ACHURJ_000773"
     CDS             complement(30332..30916)
                     /gene="phnH"
                     /locus_tag="ACHURJ_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFR1279834.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLSAPLSNDIVSQSLRFHTNAPLVNQSELATFAVTDEAIS
                     SEQLNALSSGTAVAPEAGATLILQVVSLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            complement(30913..31348)
                     /gene="phnG"
                     /locus_tag="ACHURJ_000774"
                     /pseudo
     CDS             complement(30913..31348)
                     /gene="phnG"
                     /locus_tag="ACHURJ_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313111.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
     gene            complement(31349..32074)
                     /gene="phnF"
                     /locus_tag="ACHURJ_000775"
     CDS             complement(31349..32074)
                     /gene="phnF"
                     /locus_tag="ACHURJ_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFR1279835.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGIALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            complement(32095..32874)
                     /gene="phnE"
                     /locus_tag="ACHURJ_000776"
     CDS             complement(32095..32874)
                     /gene="phnE"
                     /locus_tag="ACHURJ_000776"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFR1279836.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFVADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            complement(33000..34016)
                     /gene="phnD"
                     /locus_tag="ACHURJ_000777"
     CDS             complement(33000..34016)
                     /gene="phnD"
                     /locus_tag="ACHURJ_000777"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR1279837.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKSNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            complement(34041..34829)
                     /gene="phnC"
                     /locus_tag="ACHURJ_000778"
     CDS             complement(34041..34829)
                     /gene="phnC"
                     /locus_tag="ACHURJ_000778"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFR1279838.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRAHTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            complement(34962..35405)
                     /gene="yjdN"
                     /locus_tag="ACHURJ_000779"
     CDS             complement(34962..35405)
                     /gene="yjdN"
                     /locus_tag="ACHURJ_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFR1279839.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            complement(35565..35900)
                     /locus_tag="ACHURJ_000780"
     CDS             complement(35565..35900)
                     /locus_tag="ACHURJ_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFR1279840.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDTEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
BASE COUNT         7832 a         9709 c        10223 g         8442 t
ORIGIN      
        1 ctggagagga agagtattcc ccggtcaggc gaccggggaa ggggagagat taatgtgcgc
       61 ggccttgctc aacgccaaag ccggtctggg agcggataaa ctgcgcacgg aacagttcac
      121 gctcgcgcgc accttccgct gagttatcgg ttgccgagaa gaaccagatg ccgaggaatg
      181 ccacgctgat agagaacagc gccgggtatt catacgggaa gatggctttt tcgtgaccga
      241 ggatctgtac ccaaatagtc gggccgagga tcatcagcac cactgcggta atcaacccca
      301 gccagccgcc cagcatcgtg ccacgggtag tcagtttcga ccagtacatg gaaagcagaa
      361 tgatcgggaa gttacagctc gccgcgatgg caaacgcaag gcctaccata aaggcgatgt
      421 tctgattctc aaacagcatg ccgaggataa tcgcaatcac acccaaaatc aggacggtga
      481 ttttcgatac ccgcagttct tcacgttcgg tcgcaccttt tttgaagacg ttagcgtaca
      541 agtcatgcga aaccgcggat gcgcccgcca gcgtcagacc cgcaaccacc gcgaggatag
      601 tggcgaaagc aaccgctgaa ataaaaccga ggaacaggtt gccgcccact gcattcgcca
      661 ggtgaacggc cgccatgttg ttaccaccaa tcagatggcc cgccgcgtct ttatattccg
      721 gattcgcacc aaccagcatg atcgcgccga agccgataat aaaggtcaga atatagaagt
      781 agcccataaa cccggtggcg tagaacacgc tcttacgtgc ttcgcgggca tcgctgactg
      841 taaagaagcg catcagaatg tgcggcaagc ccgccgtacc aaacatcagt cccagaccca
      901 gagagagcgc ggagatcgga tctttcacca gcccgcccgg cttcatgatg tcgacacctt
      961 tcgggtgtac cgccatcgct tcgctgaaca gattattgaa gctaaagccg acgtgtttca
     1021 tcaccataaa ggccataaag ctggcaccga acagcaacag cacggcttta ataatttgca
     1081 cccaggtggt cgccagcatg ccgccgaaca ggacgtacat catcatcagc acgccgacca
     1141 gcaccaccgc aatgtgatag ttaaggccaa acagcagctc gatcagttta cctgcgccta
     1201 ccatctgggc gataaggtaa agcgccacca ccaccagaga accacaggcc gaaagaatac
     1261 gaatcggccc ttgtttcagg cggtaagagg ctacatcggc aaaggtgtag cgtcccaggt
     1321 tacgcagacg ttcggcaatc aggaacaaaa tgatcggcca gcccaccagg aagcccagcg
     1381 agtaaattaa gccgtcatag ccggaggtaa acaccagcgc ggagatcccc aaaaatgagg
     1441 cggcggacat atagtcaccg gcaatcgcca gcccgttctg gaagcctgtg atattgccgc
     1501 ctgccgtgta gtagtcgcta cgagaacgta cgcgttttga tgcccagtag gtaatgccga
     1561 gcgtaaacac gacgaaaatc aggaacataa taatcgcctg ccagttcgtt ggctggcgct
     1621 ctacggcccc gctaatagca tccgcggcgt tagctgcgaa agggagtgtg gcggcaagcg
     1681 ccgtcagaac tcttttcatg atgcttgtac ctcatgcagg acttcattat taagacggtc
     1741 gaattcgccg ttcgcccgcc agatgtagat accggtgaga acaaaggaga tcacaatcac
     1801 tccaacacca attggaatac cgcgtgtgac gctggtgttc ggattcagcg gcgtgcccag
     1861 ccagccgggc gcgaaggcga tcagtaaaat aaagccgata taaactgcca gcataataat
     1921 cgacaggatg gtggcaaacc gttgccgttt ttcgactaac tccctgaaat gcgcattgtc
     1981 ttctatccgc tgataaatag tgccattcat cacagattct ccagaggtaa tgtagggatt
     2041 gttttaattc ccgctccctt atgggagaag gttaacgctc gggtaaccct tgccgaatgt
     2101 aggccggata aggcgtttac gccgcatccg gcaattgtgg gttacgatgg catcgcgata
     2161 gcctgcttct cttcaagcag cttctcgact acgccaggat cggcaagcgt cgaggtatcg
     2221 cccaggttgc tggtatcgcc cgccgcaatt ttgcgcagaa tacggcgcat aattttgccg
     2281 gagcgggttt taggcaggga gtcggtccag tgcagcacgt ctggcgtcgc cagcgggcca
     2341 atctctttac gcacccagtt gcggacttct gcgtacagtt ctggtgacgg ttcctccccg
     2401 tgattaagcg tgacgtaggc gtagatcgcc tgacctttaa tattgtgcgg aatacctact
     2461 acggcggctt cggcaatctt cggatgcgcc accagcgccg actcaatctc tgccgtcccc
     2521 agacggtgac cggagacgtt cagcacgtcg tccacacgcc cggtgatcca gtaatagcca
     2581 tcttcatcgc gacgcgcgcc gtcgccgctg aaatacatat ttttgaaggt ggagaagtag
     2641 gtctgttcaa aacgttcgtg atcgccaaac agcgtacgcg cctgacccgg ccaggagtcg
     2701 gtgatcacca ggctgccttc ggtagcgcct tccagcgggt taccttcgtt atcgaccagc
     2761 gccggttgca cgccgaagaa cggacgtgtt gccgaaccgg ctttcagccc ggtagcgcca
     2821 ggcagtggcg tgatcatgaa accgccagtt tcggtctgcc accaggtatc gaccaccgga
     2881 catttctcgt tgccgatttt tttccagtac cactcccacg cttccgggtt aattggctcg
     2941 cccacggaac cgagaatgcg cagcgacgaa cggtcggtac cctcgatcgc tttatcgcct
     3001 tccgccatca gcgcgcggat cgccgtgggt gcggtataga gaatattgac ctgatgcttg
     3061 tccaccacct gcgccatacg ggcaggcgtc ggccagttgg gtacgccttc aaacatcagc
     3121 gtggtcgcac cgcaggccag cgggccgtac agcaagtaac tgtgtccggt cacccagccc
     3181 acatcggcgg tgcaccagta gatatcaccc ggatgataat caaagacata tttaaaggtc
     3241 agcgccgcgt acaccagata accgccggta gtatgcagca caccttttgg cttaccggta
     3301 gaaccggagg tgtagagaat aaacagcgga tcttcggcgt tcatctcttc cgcttggtgc
     3361 tgatcgctcg cttgctcaac caggtcgtgc caccacaggt cgcgcccttc ctgccagtca
     3421 attttcccgc cagtacgctt cagtaccacc acatgctcta cgctggtgac gttcgggttt
     3481 ttcagcgcgt catcaacgtt tttcttcagc ggaatactgc cgcccggcac gcacaccttc
     3541 gtcggaagtg atcaccagtc gtgagttgga atcaataatg cgcccggcaa cggcttccgg
     3601 cgagaagccg ccgaaaatca ccgaatgcac cgcgccaatg cgggcgcagg ccagcatcgc
     3661 aaccgcggct tccggcacca tcggcatata aatcgccacc acatcacctt ttttaatgcc
     3721 cagctcgagc agggtattgg cgaagcggca gacgtcgcgg tgcagctctt tatagctgat
     3781 atgtttgctc tggctggcgt cgtcgccttc ccagatgatg gcggtacgat cgccgttttc
     3841 ttgcagatgg cggtcaaggc agtttgccgc cagattcagc gtgccgtcct cgtaccattt
     3901 aatggacaca ttaccggggg caaaggaggt gtttttcacc ttctggtaag gtttgatcca
     3961 gtcaagaatt tttccctgtt cgccccagaa ggtatcaggt acgttaatag attgttgata
     4021 cattgcctcg tactgctgag ggtttatcag gcaacggtct gcgatgttgg caggaatggt
     4081 gtgtttgtga atttggctca tgcttttgtt ctccttgtag aatgttaata atatgtgaca
     4141 taagcgttaa atgtaggggt attggcagtt ttgtttagta tttgggcgac agatcacgca
     4201 aaagtagaat tgtgcaaata aacggcaggg taatttttga aggtcagcaa caaaagttga
     4261 ttaattcttt gaggaacatg cagttatgca tgctgttgaa aaagaggaag atactgacta
     4321 actctaaagt ggtattttac atgcacttac aattgattaa agacaacatt catagtgtgg
     4381 ttatttgtta cacatagggg cgagcaatgt catgacagtg taggtgcggt tactgtcgtg
     4441 aaaaacaata aaaaccgcca ttgcaacaat ggcgcaattc ggatgaagcc cctatgacaa
     4501 ggataaaaat aaacgcacgc cgtatcttca gcttattgat tccttttttc tttttcactt
     4561 ctgttcacgc tgaacaaacg gcagctcccg caaaacctgt aactgtggaa gcgaagaatg
     4621 aaacctttgc cccgcagcat cccgatcaat atctctcctg gaaagccacc tcggaacagt
     4681 cagagcgtgt tgacgccctg gcggaagatc cacggctggt gatcctgtgg gcggggtatc
     4741 ccttctcgcg cgattacaac aagccgcgtg gacatgcctt tgctgtgacc gatgtgcgtg
     4801 aaaccctgcg taccggtgcg ccgaaaaatg ctgaagatgg tccgctaccg atggcgtgct
     4861 ggagttgtaa aagcccggat gtggcgcgtc tgatccagaa agacggcgaa gatggctact
     4921 tccacggtaa gtgggcgcgc ggcggcccgg aaatcgtcaa caacttaggt tgtgccgact
     4981 gccataacac cgcctcacca gagttcgcca aaggcaaacc ggagttaacc ctttcccgtc
     5041 cgtatgcggc tcgcgcgatg gaagccattg gtaaaccttt tgagaaagcc ggacgtttcg
     5101 accagcaatc gatggtttgc ggtcagtgcc atgtggagta ttacttcgac ggcaaaaaca
     5161 aagcggttaa attcccgtgg gatgacggca tgaaagtcga aaatatggag cagtattacg
     5221 acaaaattgc cttctctgac tggactaact ccctgtcgaa aacgccaatg ctgaaagcgc
     5281 agcacccgga atatgaaacc tggacagcgg gcattcacgg taaaaacaac gtgacctgta
     5341 tcgactgcca tatgccaaaa gtgcagaacg ccgaaggcaa actctacacc gaccataaaa
     5401 ttggtaatcc gtttgataac ttcgcccaga cttgtgcgaa ctgccatacc caggacaaag
     5461 ctgccttgca aaaagtggtc gcggaacgta agcagtcgat taacgacctg aaaatcaagg
     5521 ttgaagatca actggttcac gctcacttcg aagcgaaagc agcgctggat gcaggcgcga
     5581 cggaagctga aatgaagcca attcaggacg atatccgtca tgcccagtgg cgctgggatc
     5641 tggcgatcgc ttcccacggc attcatatgc acgcaccgga agaaggttta cggatgctcg
     5701 gtacggcgat ggataaagcg gcggatgcac gcaccaaact ggcgcgcctg ctggcgacca
     5761 aaggcatcac ccatgaaatc cagatcccgg atatctcaac caaagagaaa gcccagcagg
     5821 ccattggcct gaacatggaa caaatcaagg ccgagaagca ggacttcatc aaaacggtga
     5881 tcccgcagtg ggaagaacag gcacgtaaaa acggtctgtt aagccaataa ccccgttccg
     5941 cctcgcaagg ggcggaaaac acaatggagt gaatatgagc gtattacgtt cgttattaac
     6001 tgctggggtg ctggcgtcgg gcctgttgtg gagcctgaac ggaattaccg ctacccctgc
     6061 ggcgcaggca tctggtgatc gctacgaagt tacccagcag cgtaacccgg atgccgcctg
     6121 tctggactgt cataaaccag ataccgaagg tatgcatggc aaacatgctt ccgttatcaa
     6181 cccgaataac aaattgccgg tcacctgcac caactgtcac ggccagccat caccgcaaca
     6241 ccgcgaaggg gtgaaagatg tgatgcgctt taacgagccg atgtacaagg tcggagagca
     6301 gaacagcgtc tgtatgtcct gtcatctgcc ggaacagttg caaaaagcgt tctggccgca
     6361 cgatgtccac gtaaccaaag tggcgtgcgc cagctgccat tccctgcatc cacagcaaga
     6421 tacgatgcag acgttaagtg acaaaggacg gattaagatt tgcgtcgatt gccacagcga
     6481 tcagcgcacc aatccgaact ttaacccggc gtctgttccg ttgcttaagg agcagccatg
     6541 acctggtctc gtcgccagtt tctcaccggc gtcggcgtgc tggcagccgt cagcggcacc
     6601 gcagggcgcg tggtggcgaa aacgttgaat atcaatgggg tgcgttacgg catggtgcat
     6661 gacgagtcgt tatgcatcgg ctgtaccgcc tgtatggatg cttgtcgaga agtgaacaag
     6721 gtgccggaag gcgtctcgcg cctgacgatt attcgtagcg agccgcaggg cgaatttcct
     6781 gatgtgaaat atcgcttctt ccgtaagtct tgccagcact gcgatcatgc gccgtgcgtt
     6841 gacgtctgcc cgaccggtgc gtcttttcgt gatgccgcta gcgggattgt cgatgttaat
     6901 cctgatctct gcgtcggttg tcagtactgc atcgccgcct gtccgtaccg cgtgcgcttt
     6961 atccatccgg tcacgaagac ggcggacaaa tgcgatttct gccgtaaaac caatttgcag
     7021 gccggtaagc tgcccgcgtg cgttgaggct tgcccgacga aggcgctgac gtttggcaat
     7081 ctggacgatc ctaacagtga gatttcgcaa ctgctgcgcc agaagcctac ttaccgctac
     7141 aagctggcgc tgggaaccaa accgaagctg taccgcgtac cgtttaaata cggggaggtg
     7201 agccaatgac gcagacttcc gcatttcatt ttgaatcgct ggtgtgggac tggccgattg
     7261 ccatctacct gtttttgatt ggtatttctg ccggtctggt gacgctggcc gtgctgttac
     7321 gtcgcttcta cccgcaggcg ggcggtgcag acagtacgtt gctgcgcacc acgctgattg
     7381 tcgggccggg cgcggtgatc ctcggtctgt tgatcctcgt cttccacctg acaagaccgt
     7441 ggaccttctg gaagctgatg ttccactaca gttttacctc ggtgatgtcg atgggggtga
     7501 tgctgtttca gctttacatg gtggtgctgg tgctgtggct ggcgaaaatc tttgaacatg
     7561 atttgcttgc ccagcaacaa cgctggttgc cgaagctggg gatcgtgcaa aaggttctga
     7621 gcctgctgac gcccgttcat cgcggactgg aaacgctgat gctggtgttg gcggtgctgc
     7681 tgggggctta taccggcttt ctgctgtcgg cgctgaaatc gtatccgttc ctcaataacc
     7741 cgatcctgcc ggtgctgttc ctcttctccg gcatctcgtc cggtgcggcg gtggcgctga
     7801 tcgccatggc gatacgccaa cgcagtaacc cgcattccac ggaagcgcac tttgttcacc
     7861 ggatggaaat ccccgtggta tggggtgaaa tcttcctgct ggtggcgttt tttgtcggtc
     7921 tggcgctggg cgatgacggt aaagttcgtg cgctggtggc ggcattaggt ggcggtttct
     7981 ggacgtggtg gttctggctt ggtgtcgccg ggctggggct gattgtgcca atgttgctca
     8041 aatcgtgggt caatcgcagt tccggcattc ctgccgtgct ggcggcgtgt ggggccagtc
     8101 tggtcggcgt gttgatgctg cgctttttca ttctctacgc cggacagttg acggtggcgt
     8161 aagccagaaa agaggtggtt tctggacgta ttccttcctg aagtcggttt tctggcgttg
     8221 ttgttaagtc tcggggtcaa cgtgttgacc ccgttgacgg cctttgcggg agtgcggttg
     8281 cgctggcctg ccatgatgcg actcacttgc atcggcattc tggcgcagtg cgcgctcctg
     8341 ctgctcgcct ttggcgtact gacgtattgt tttctcatca gcgatttctc ggtcatttat
     8401 gtcgcccaac atagctacag cctgctgtcg tgggaactca aactggcggc ggtgtggggc
     8461 ggtcatgaag gttcgctgct gctttgggtg ctgctgcttt ccgcctggag cgcgctgttt
     8521 gcctggcatt atcggcagca aaccgatccg ctatttccgc tgacgctagc cgttttatct
     8581 ctcatgctcg ccgcactgct actgtttgtg gtgctgtggt ccgatccctt cgtgcggata
     8641 tttccaccag caatcgaagg ccgcgatctc aatccgatgc tgcaacatcc cggtcttatc
     8701 tttcatccac cgctgcttta ccttggctat ggcggtttga tggtagcggc gagcgtggcg
     8761 ctggcgagtc tactgcgcgg cgagtttgat gcggccagcg cccgaatttg ctggcgctgg
     8821 gcgttacctg gctggagtgc attaacggcg gggatcatcc tcggttcctg gtgggcctac
     8881 tgcgaactcg gctggggcgg ctggtggttc tgggatccgg tggaaaatgc ctctttatta
     8941 ccctggcttt ctgccactgc gctgctgcac agtttatccc tgacacgcca gcgggggatt
     9001 tttcgccact ggtcgctgtt actggcgata gttactctga tgctgtcgct gctgggcacc
     9061 ttaattgtcc gttctggcat tctggtttcg gttcatgcgt tcgcgctgga taacgtccgc
     9121 gccgtgccgt tgttcagcct gtttgcactg attagccttg cgtctctggc tctgtatggc
     9181 tggcgagcgc gggacggtgg cccggcggtg cgtttttcgg ggttatcgcg ggaaatgtta
     9241 atcctcgcta cgctgttgct gttttgcgca gtgctactga tcgtgctggt gggaacgctt
     9301 tatccgatga tttacggtct gctgggctgg ggacgcctct ccgttggcgc gccgtatttt
     9361 aaccgcgcga cgttaccgtt tggtctgttg atgctggtgg tgattgtgct ggcgacgttt
     9421 gtctctggca aacgcgtgca gcttccggcg ctggtagctc atgcgggcgt gctgttattt
     9481 gccgctggga tcgtggtttc cagcgtcagc cgtcaggaga tcagcctgaa tttacagccg
     9541 ggtcagcagg tgacgctggc aggatacacc ttccgttttg agcgcctcga tctgcaagcc
     9601 aaaggcaatt acaccagcga aaaagcgata gtggcactgt ttgaccatca gcaacgcatt
     9661 ggtgaactga tgccggagcg gcgtttttac gaagcacgtc gtcagcaaat gatggaaccg
     9721 tcaattcgct ggaacggcat ccatgactgg tatgtggtca tgggtgaaaa aaccggagcg
     9781 gatcgttacg cttttcgctt gtatgtacaa agcggtgtgc gctggatctg ggggggagga
     9841 ttgttgatga ttgcgggcgc attgttaagc ggatggcggg ggaggaagcg cgatgaataa
     9901 aggacttctc acgctactgc tgttatttac ctgttttgcg cgcgctcagg tcgtagacac
     9961 ctggcaattc gccaatccgc aacaacagca acaggcgtta aatattgcca gccagttacg
    10021 ttgtccgcag tgccagaatc aaaacttact ggaatccaac gcgccggtgg cagtcagtat
    10081 gcgccatcag gtttacagca tggtggcgga ggggaagaac gaagtcgaaa tcattggctg
    10141 gatgaccgaa cgctacggag attttgttcg ctataaccca ccgttaacgg gtcagacgct
    10201 ggtgttgtgg gcgctgccag tggtgttgtt actgctgatg gcactgatcc tctggcgagt
    10261 gagggcgaag cgatgaaaga gcccaaaata ccggtgaaaa tgctgacaac actcacgatt
    10321 ttgatggtat ttctctgtgt cggcagttat ctgttaagtc caaaatggca ggcggtacat
    10381 gcggaatatc agcgtcagcg cgatccgcta catcagtttg ccagccagca aaccccggaa
    10441 gcgcagcttc aggcattgca ggataaaatc cgcgctaatc cacaaaacag cgaacaatgg
    10501 gcgttactgg gcgagtacta tctgtggcaa aacgattaca gcaattcgct gctggcgtac
    10561 cgtcaggcgt tgcaactacg tggtgagaac gctgaactgt atgcggcgct ggcgacggtg
    10621 ctttattacc aggccagcca gcatatgacc gcccagactc gcgcaatgat cgacaaagcc
    10681 ctcgcgctgg actctaacga aatcaccgct ctgatgctgc tggcttccga tgcgtttatg
    10741 caggcgaatt acgcgcaagc catcgaacta tggcaaaaag tgatggatct caactcaccc
    10801 cggatcaacc ggacacagct ggtcgagtcg attaatatgg cgaaattgtt gcagcggaga
    10861 tcggattaat catctggtga tgaagattat gttattgagc tgcataaaaa ataatcgaat
    10921 gaacatatgc caaaaataat cactaatcag tattattgca gattaaacaa ataaaaatct
    10981 ttccataaca aatggttatt cattaatcct gacataaact cgcgttatta tgcattaatg
    11041 cagcgaaaag ctctgttgtt aaagggttgc gcaacatacc gcgcaaatga tactgatcat
    11101 aagcgttaaa aaaatctaca aaccaacgca acacaattca tgcctggcag tatgtcacgt
    11161 tctcgcgttt ctgaacgggg aacggcgctc cattgaggaa gttattctga tgaaaaatat
    11221 aaaattcagc ctggcctggc agattctgtt tgctatggtg ctgggcattc tcctgggaag
    11281 ctacctgcac taccatagcg acagccgcga ctggctggtc gtcaatttgc tctctccggc
    11341 gggtgatatc ttcatccatc tgattaaaat gattgttgtg ccgattgtga tctccacgct
    11401 ggtggtgggt atcgcgggtg tgggtgatgc caaacagctc gggcgtattg gcgcgaaaac
    11461 cattatttac ttcgaggtga tcaccaccgt cgccatcatt ttggggatca ctctggcgaa
    11521 cgtcttccag cccggtgccg gggtggatat gtcgcagctg gcgaccgtcg atatctcgaa
    11581 atatcagagc actacggaag cggtacaaag cagttcccac ggcattatgg gcacgatttt
    11641 gtcgctggtg ccgacgaaca ttgtggcgtc gatggcgaaa ggcgaaatgc tgccgatcat
    11701 cttcttctcg gtgctgtttg gtctggggct ttcctccctg cccgcgacgc atcgtgaacc
    11761 gctggtgacc gtgttccgct ccatctctga aaccatgttt aaagtgactc acatggtgat
    11821 gcgttatgca ccggtgggtg tgtttgcgct gattgcggtg acggtggcta actttggttt
    11881 ctcttctctg tggccactgg cgaaactggt gctgctggtg catttcgcca ttctgttctt
    11941 cgcgctggta gtgctgggaa ttgtggcgcg cctgtgcggg ttaagcgtct ggatcctgat
    12001 tcgtattctg aaagatgagc tgattctggc gtactccact gccagctctg aaagcgtact
    12061 gccgcgaatt attgagaaga tggaagccta cggagcaccg gcgtcgatca ccagcttcgt
    12121 ggtgccgacc ggttactctt ttaaccttga tggttcgacg ctgtatcaaa gtattgccgc
    12181 tattttcatc gctcagctgt atggcattga cctgtccatc tggcaggaaa tcattctggt
    12241 gctgacgctg atggtgacct cgaaggggat tgctggcgtg ccgggcgtgt cgtttgtggt
    12301 gttgctggca acgctgggta gcgtaggtat tccgctggaa ggtctggcgt ttattgctgg
    12361 tgttgaccgt atcctcgaca tggcgcgtac tgcgctgaac gtggtgggta atgcgctggc
    12421 ggtgctggtg attgccaagt gggaacacaa atttgaccgc aaaaaggcgc tggcttatga
    12481 gcgtgaagtg ctgggcaaat ttgataaaac tgcggatcaa taattgaaga ttgccggggt
    12541 tatccacccg gcaatgtgtg gatgcctgat gcgacgctgg tgcgtcttat caggcctacg
    12601 ccagacagcg caatagcctg atttagcgtg attttgtagg tcggataagg cgtttacgcc
    12661 gcatccgaca tcaacgcctg atgcgacgct taacgcgtct tatcaggcct accttaaccg
    12721 ttggttaatt tttcaaactc ttcacacccg gtatcaaacc cttccataca gctcaggttc
    12781 aaccagtgca gcgccttttg tttgttcttc tcgataaaac cttcttcacc gtttaagaac
    12841 atcattcccg cccagtactc ggaataaccg gttcgggaaa ttgcagagct gcgtttgaaa
    12901 tactaggttg ccttgtcatc gtcggcctta atacccacgc cgttggcgta aatcaaacca
    12961 agcagcattt gggcatcgac ggcagagtcg ttctccagat cttccgaggc ttttctaaca
    13021 gcgaaatcgc ctttggataa tccggtttac ccgcttgagt atttaccaga atatgcgcca
    13081 gcgttacttc accttcttta ctgcccgctt gcgccgcttt ctctgcaaga acttttgctt
    13141 gtggatagtc cagactgccc ggattggtga ttttgatctg cgccaacagc gcgcaggcat
    13201 cagcatcacc gctgtcggcg gctttctgtg cccaatattc ggctttgctc aaatcgccgg
    13261 agctaaacca gctgtcggca agaaaatatt gtgcgcgtcg gtccccggcc tctgctgcct
    13321 ttaaatactg gctgccaggc tcggagtcgt tggcgtgggc aaagaatgtc aaaaacaaca
    13381 tcaatgcgat aatttttttc attttgataa tacgattggg tagggagtaa ccagtataaa
    13441 gagttcgaat ggataaaaaa acagcctccg aaaggaggct gtagaaagga ctgtattacg
    13501 ccagtgccgc ttcgcgcagg cgagttttca acttgttgta ctcgtcaatc acgtactgct
    13561 cggcggcgcg ctgatcggcg atcggctcga cgcgcacggc gcagtatttg tattccggcg
    13621 ttttcgtaat cgggcttaag ttttcggtaa ccagctcgtt acaggaacca atccaccact
    13681 ggtaggtcat gtaaatcgcc cctttgttcg gacgatcgct gacctgcgca cgggtgataa
    13741 ttttgccttt acgcgagtgc acccaaacca atgcctcatc ttcaataccc agacgtttgg
    13801 cgtcttcggt attgatttgt gcgtagccag gttcatcagc cagcgccgcc agtgccgcac
    13861 agttaccggt catcgaacgg caagagtagt gaccaacttc acgcaccgtt gacagtacca
    13921 tcgggtactc gtcggtgagt ttgtcgattg gcgctaccca gtcgcaggtg aagaactgcg
    13981 ccagaccgtt cggggtatca aacttctctt taaacagata agaagtcccc tgatcggcat
    14041 ctgaagtatc gcggcaaggc cactgaatga agccctgttc gcccattttc tcgtaagtcg
    14101 caccgtagaa atccgggcac agatgacgca actcatccca gatctcctgg gtgttgttgt
    14161 agtgcatcgg ataacccata cgggtggcga tttcactgat gatttgccag tccgttttca
    14221 gatcccattt cggttcaact gctttgaaga aacgctggaa gccacggtca gccgcagtaa
    14281 acacgccttc atgctcgccc cacgacgttg acggcaaaat gacatccgcc gccgacgcgg
    14341 ttttggtcat aaagatgtcc tgaacgatga ccagttccag atcttcaaag gctttacgta
    14401 ctgccgacag ctccgcatca gtttgcagcg gatcttcgcc cataatgtac gcggcacgca
    14461 cttcgccatg cgctgcgcgg tgcggcagct cgctgatgcg atagccggta tgagcaggca
    14521 ggctttccac gccccaggct ttggcgaatt tctcgcggtt agccggatct ttcacgtact
    14581 ggtatcccgg atacgtatcc ggcagcgcgc ccatatcgca ggcaccctgc acgttgttct
    14641 gaccacgaac cgggtttaca cccgcatgcg gcttaccgag gttaccggtc agcatcgcga
    14701 ggctggtcag agaacgcacg gtttccacgc cctggtagaa ctgggttaca cccatgcccc
    14761 acaggatggc ggcgcttttc gcctgggcat acatccgtgc cgcctgacga atctcactgg
    14821 cgctgacgcc ggtgatatct tcaaccgact ccggcgtgta gccttcaacg attttacgat
    14881 actcttcaaa gccttctgta cgggaagcga cgaacgcttt gtcgtacaga ttttcttcaa
    14941 taatgacatg gcccatcgca ttcaacagcg cgatgttcga gccgtttttc agtgcaatgt
    15001 gcatgtcagc aatgcgcgcg gtttcaattt tgcgcggatc gcagacgata attttcgccc
    15061 cgttacgttt agcgttaatt acgtgattcg ccatgattgg gtgggaatcc gccgggttgt
    15121 acccgaaaac gaacactaaa tcggtattat caatttcgtt aatagcattg ctcattgcgc
    15181 cattaccgac cgattggtgc agacctgcaa ccgatgggcc gtgtcagaca cgagcgcagc
    15241 agtcaacgtt attggtacca ataacggcgc gcgcaaattt ttgcattaca tagttggttt
    15301 cgttacccgt accacgcgag gagccggtcg tctggatggc atccggaccg tacttctctt
    15361 tgatggcgct caggcgctcg gcaacgtaat tcagtgcctc atcccaggaa acaggttcga
    15421 gtttgccgcc acgctggcga cggatcatgg gggttttcag gcgcggggtc aggatctggg
    15481 tatcgttaat gaagtcccag ccataataac ccttcagaca cagggtaccc tggttggttt
    15541 tcccctgcgc tgcctccgcc cggacgattt tgccgttatc gacgaccagg ttgattttgc
    15601 aacctgatgc gcaatagggg caaaccgtga cgactttttt catcggtctc gctccagtta
    15661 atcaaatcac gcatacgcgc tctcgactac agtatgcatc ttttatgcca cattttattt
    15721 ggggtcattc cctgatatta cgggcactat ttattcaaaa ctctgacgaa aaacaggctg
    15781 tcgtcagttt tgacgtgacg aaacgaaata ccgcgtgaca gccatcacgc ggcagacatt
    15841 ttattttttc tcgacgacgg gagccgcctg atacccgccg cccagcgatt tcatcaactg
    15901 aatgctgtgg atcacccggc ggctgtccag catcagtaat gacatctctt cggcaagcac
    15961 tggcaaccgg gcttcggtgg cctgtaagcg gctggttaag ccgcgctgat aggcggcctc
    16021 ggcagcgcgc tgggtgaagc gcgttgcttc cacgcgttca gcctgcattt ctcgttcgtc
    16081 gttgagcgtt tgcagacgcg tgccgttgac ggcaacgtca cgcaccgcgt tcagtactga
    16141 ctggttgtaa cgttcaatca tcatgttgct ggcggcgcga gtgccttcga gattggcatt
    16201 caaccgtcca ccgtcaaaca gcggcaattt cagacccggg atgaagttga actggcgact
    16261 ggtttttttg aataaggtat ccaggtggat ggagtccaga ccgaaaaacg ctttgatatc
    16321 aaagctcggg tagaacagtg cccgcgcgga atccacctga tctaatgacg cctgaacata
    16381 ccagcgcatg gcttgcagat ccgggcgtct ggcgagcaac tcataagaga gtgtcgccgg
    16441 aatgccggtc tggactcgtg gtaatgccac cggtttgatc tccggcatat cgctggcccc
    16501 cgcgccaatc aatgcacgca gagattctcg cgtttcggtg atttgccctt tgacggcagc
    16561 aatttgttta tcgaccgcca gaatctgtgc ccgcgcgccg tggaaaggca cttgtgcttc
    16621 cagaccgtgc gccactttac tctggtgcgc tttcaccgcg taatcaatca catcgcgagt
    16681 ttgttctaac agatcgagca tctgatagct ggcctgcata ctgtaataaa gctgcgctac
    16741 gcccgtggtc agcgatagct ctactgctgc ggtttctgcc agcgcggcat tatgcgcgcc
    16801 aatggcggcg gcaaccgctg agcgatgcac accccacaaa tcaagatcca gtccggcaaa
    16861 caaacctact gtggcttccg tatagtacgg cccgtccata cccagtgagg gcgcatccat
    16921 cgcataaggg cttaaaaagc cgttcgccga gacgcgttgg cggttgagca tccctaacgc
    16981 tgcgacctgt aattgtgaac cggcatctaa caaatcggcc tgggactgcg ctttttcttc
    17041 ccgcagtttc gcttcggcga gggtgtgtga accacttagc gtccgttgga tcagcgcatc
    17101 cagctgcggg tcattgagtt gtttccacca ctgcgcctgc ggccagccgg agctggcaag
    17161 atgaatatcg tcggccagtt tgatttgttc cggtttgagc tgttgatgag gtgcagaatc
    17221 cttacgtacc agggcacagc cggaaatcag cgtcgtgctg ccgagaatgc tgcacaacag
    17281 cagacgtgaa agttgacgat tgatcattgc gtggctcctt gtgcctgttc cgtggcgggc
    17341 gttaatgccg gggctgtcca gtctggcaag cgggcgaaaa gctggcagac gtgttgctcc
    17401 tgttttaata aggtgggcag gatggcctgc gaggcgggtg tctcttcaag cgttatttga
    17461 ggagaacagc tgagtgcggt tgccagaccg gcagcgtatt tttccagggc gtcggcaaac
    17521 tgacctgctc tgtccggctg tagtgcgtta tccagcgcct gcgccgagtt ccaggtggca
    17581 tcccaggcgt gaagaagatc gcggccctga cgaataaccg tttgcgaacg ttcaatcagt
    17641 aaggcgcgtt cttcgctgtc cagttgacgc tccagcgcca cgcgttggca catctcttca
    17701 caggcattaa acgccgcatg cagacctata cgaatttgca gataagtgcg cagagccgtg
    17761 acttcctgct ggcgtgggat ccgcattact ttacttagca tacccagcgc accggctagt
    17821 ttttgcggca gagtgcgcgc ttcactttca ggccagacaa aggtgtaaat caccgcggac
    17881 accacggtac caatgatgat acccagggcg cgatcgcgaa tttccaccag gtcgtacact
    17941 gggccaaaaa cgttttcgag cgtggcgagc gcgaaggtga cgaccatctg tgtgccgata
    18001 taagaagagc gttcagagct ggtggcaatc catgcgccca acaggaaaat cggtgccagc
    18061 acaaacagca attcgacaat attgtccagc cagggcatga ccagtagcgt gaataacagc
    18121 gccagaatcg cgccgcaaaa ggccccgcca aaacgcagca ccatcttctg gtacgacgaa
    18181 ccgacatttg gattagcgac gatcacgcag gtcagcatac aggtgtgaat gccttcccag
    18241 tccacgccgc tgtaaaaggt gtaacagatc aaacaggcga gcaatgtttt taccgcgtag
    18301 cgtatatagt ctggattggt aaaagcatcg gcgaccattg atggcggttt ggcggcgggc
    18361 gttggcggcg tattcgggtt catctggccc agttgtaaca acgtctggca gatattctcc
    18421 aggttacatt cccgtgccgc caccgcttca ctttcactga tccgccagtc gctttgccag
    18481 cactgacctt cagcaacggc atgctgcagc ttgttgattt ctgaggctaa tttttgccga
    18541 aattcaataa ttgcctgaga atcagcaaaa gaggtgggat cgtagcgatt cagcgtcgag
    18601 taaatgtagg ttaccgttgc cacgcagctt tgccaccatg cgctttgagt tcgccagttg
    18661 gcatcgtccg cgagacaaaa gacattcagt ttttgcagcg ccagcgcctc tctttcaatc
    18721 cgcgtttcgg gtagcggtgc gaggctgtct gtcaggtgac taatggcatc atcaagccga
    18781 tcattaagcg cctgatgcat ttgcgaaatg gcacgactgg gaaaccacag cacgccgatt
    18841 aacgtcatca gcaaggttgg atagaggcca acaacgatac accacagcgt taagcgcacg
    18901 accacttccg gatagtcgag catggcgggg aaggtttgcc cgtaaatggc gacaatggcg
    18961 acagcgaaaa agaccagtcc cagccgatgg gtgcgcatca aaaacatgca gcccatcagg
    19021 atcggcccgg cgatgatcaa tcggatcaac ggttcgccgt atgaccattt gtagatcaaa
    19081 aacaggctgc cgatctccag caccgtggca accacaaaca agatcgccac aaatttggtg
    19141 taaaacgcgt tcgactgaat accgtaaaac agcactgcca gcgataacgc cacaaaaggg
    19201 atctcaaaag tcatcgagat cagaatcacc agcaggcagc ctacccagag ttgcacgatc
    19261 tgcggcacgc ggccgggtcg ccgctcgctt aactcttcat gaaagaacgc cagcagcctg
    19321 accaccggta atggcaggga gttgagcgcg ctcattattg cggctcaaga ttagcgactg
    19381 ccgaagcgcc gatgcggaac atttccgggt caggtttatc gaccatgatt ttgaccggaa
    19441 aacgctgggc aacgcggacc cagttaatag agcgagaaac tttcggcagg ccgcccagca
    19501 ccaggccgcc gtcattcggt agcacaccgt agccaatcga atccacttta ccctcgaagg
    19561 ttttgccgct gtcactcatc aggcgaatcg ttgcgggtgt acctgagcga atatttttca
    19621 gatcggtttc gcggaagttg gcgatcacgt accagtgacg agtgtcgatt agggtaaaaa
    19681 tagggcgcat ggcagaagca aattgcccga cggaggtttt gagggaaatg acccggccat
    19741 caaacggcgc gcgaacggtc gccatttcca gatgcagttt ggtcagggca atatccgctt
    19801 cgaccgccgc acgctgggca actaatacat ccacgccgct gacggcgctg gcggctgact
    19861 gcgcctgtaa caatacggca ttaagatccg cttctgcggc gcgctgcgcc gttcttgcac
    19921 ggtcaacatc ttccgctgag acaaaacctt ctttcagtaa tggctcggtg cggcgtaatg
    19981 tatctgtggc ctgtttcgcg gcggcacgtg ctttttctac cgtggcatta accgagtcgg
    20041 caccaaactg ttgggcgtca acgctacgct gggtgagcat aatttgctta tccagcgccg
    20101 cgagggaggc ttcagctttc gccagattgg cttcgtacgg gcgcgggtca atgcggaaca
    20161 gcaaatcgcc ctgtttgact gcctggttgt cggtgaccgc cagttctaca atgcggccgc
    20221 tgacttccgg caccacatca atggtatctg ctgacgcgta agcgtcatta gttgatggcg
    20281 cactgtctac gcgccagata acgaaaacaa gggcaaccag cgccaacgca accactaaca
    20341 gagcagggaa tttactgcga ggagcttttt tcggcgtact ttccatgagc gcgtctcaaa
    20401 tagattagaa aaatgccagc cagaacagca tggcgtagac agcaaaaagg gcggtataaa
    20461 taattccgac aaatgccaga ttgatatttg cccgctgaat aattcgcctc gtgattagcg
    20521 tcaaaataag gctggcaatg gcgcagaaaa accagctggg ataataagcg ccgatcaccg
    20581 ggattgccgg agataatgag catcccgaaa ccatgaagac gggaattatc caggctgttt
    20641 ttttgagttg catcgccatg cgagagagaa ctgttggcat attaaataca tcctgtttgc
    20701 tgcgaacaat atctgtagtg aatatcaata tccattgaag gcgtcagtat ggctattttc
    20761 ttgatgaata aaaatagctt tattcagcca ccgcattcat aacttatata ttttatgtgt
    20821 taattgattg tatgtatttt tataataaat gcttttgggg gcttaagcgc ccccaaaagt
    20881 gagtggcatt aatttggggt tacgatattc acccacaaat cgaaattatc cagacaggca
    20941 ataatttctt ccagtttcgc gccattgcca ataattttgg ctttcttcgc ttttaccaga
    21001 tccgccattt tggcttgtcc tgtcagcacg gcgtgcagat cttcacggct gatgtagaaa
    21061 gaggcgtcgg cttgcggttg cagtgtttta cggtagttaa gcacgctatc gttcagcgtc
    21121 aggttgaggt tatcgccgtt gctcatattg aagttcaggc tgatattttt acccgcagct
    21181 ttcgcgctat cgaggcgaac ggacataaag tcgaacagca tttcgaccga catcccgcga
    21241 atggtgtccg gggaaccggt ggtgccgtgg ctgaacttat gcaccccttc gcgcagctct
    21301 ttcgcgccgg tcaggtagaa accgcgccag gtggcggact cggcctgata gcccagctgt
    21361 tcaaagttat tcgcttgcag attcttcgcg acctggtcac ccgggttggc ggcaatcacc
    21421 tgtttcagca gttctgccga ccagcggtaa tcaccttgct tgttcgcttc ttgcgccagg
    21481 ctgatgacac gggcagaacc gcccagcgcc tgcacgtaac gtttacccat ctccacctga
    21541 ccatacggat gcaggttagc cgggttaccg tcgtaatagc caagatagaa gttatacacc
    21601 gcgcgggcgt tgtggctgac agaaccgtaa tagccacggc tggcccagtt attggcaagt
    21661 gcaggcggca gtttaatcat gtcgccgatt tcattcatgg tgtagccctg gttcgccagg
    21721 tgcagggtct ggtcgtgaat gtacttgata gtatcgcgat atttaccaat ataatcattg
    21781 atatgcttat tgccccggac cggccaggtg tgcggcataa acaggacttc cgcgtcgtta
    21841 ccccacatat ccagcgtttc gttcagatac tcggtccact tgctggtgtc gcgggttttc
    21901 gcgccgcgca gagtgtagaa gttgtgcagg gtatgcgtgg cgttctcggc ggtacacagc
    21961 gctttcaggg ccggaatata gaagtgcatt tcggctggtg cttcgctacc tggggtcatc
    22021 aggaagtcaa actccaggcc gtcgataatc atcttctcac cggttttggt gatggttttg
    22081 ttcggtgcga tgatgctcgg gtagcccgat gccagcgtta cgccaagacc attgccgatg
    22141 ttaccctgag ggttatgcgc cagcagcagg ccgtaggagt aaagtgcacg gtggctcata
    22201 atattacccg ccagtacgtt ttcgctgatg gcttcgtcca taaagccagc aggggcgata
    22261 acctgcactt taccggattt cacatcagct tcagagataa tgcctttcac gccgccatag
    22321 tggtcggtat ggctgtgggt ataaataacc gcaacaatcg gtttttgcgg gcgattttgg
    22381 aagtaaaggt caagggcggc ttttgcgctc ggtggcgtca ccagcgggtc gatgacaata
    22441 atcccggtat caccttcgat aaaggtgatg ttggagatat cctgaccgcg aacctggtac
    22501 attctgtcgg tgactttaaa caggccggaa ataccgttta actgcgactg acgccacagg
    22561 ctagggttaa cggtttcggg cgcgggcgca ttgatatcaa atttataatc ttccccgcga
    22621 taatatggtt tgccattagc atcgcgcaga ataccttcat ccagcagcgg agcgataaat
    22681 ccacgctgag cgtcgtcaaa atcctgacga tcgctaaacg gtaatgattt ggcgtaattc
    22741 tgattaacct gttgggtata ttgagtggcg gctttcgctt cctctttcgc gttagcggta
    22801 tttaccatca tgccgctggc ggcagttaac gcaataacaa ttctgctcaa acggaataac
    22861 cgagagttat tcatataaat ctccatttat catttatgtc gtaaatatgt aatccataca
    22921 aaatacgtca tttaaagctg gggttatcat aattgggaat ttgcgacttt aataagtgga
    22981 agtgtgagct gaacgatcca ttttattagg catttgtgct gaagagagaa aattacgcga
    23041 atttattatc tctgtaaata atatgaggtg ttttatatat ttattatata tattgtactt
    23101 cggcaaatac tgagcacaat ttatcaatgc aaccggtgtt tctttttaaa aaacgtaccg
    23161 gagcaatggg cggtcatgtc gctggcaccg caggattctg cgttgctgtg ccagcctttt
    23221 attcatggtc aatgagtgag gtgattgata aagacaccag gtcgtcagag cgcactgatt
    23281 atccgcatta tgccagtgaa gttgcactgg cttttggttc acgagtggtt ggcctcgaac
    23341 tggcaaaaat gattgtggat gcgtggctgg gcgcacagta cgaaggcggt cgtcatcaac
    23401 aacgcgtgga ggcgattacg gcaatagagc agcggagaaa ttgagattca tccactactt
    23461 gcatggatga gtaatgatta atgtggatag agtttctttt tgaggttggc taatgaaacg
    23521 ctttccgcta tttcttttat tcaccctgct cacgctgtcc accgttccgg cccaggccga
    23581 tattatcgac gacaccattg gcaatattca gcaggcgatt aacgacgctt ataaccctga
    23641 ccgtgggcga gactatgaag attcgcgcga cgacggctgg cagcgtgaag tgagcgacga
    23701 tcggcgcaga caatatgacg accgccgccg ccagtttgaa gatcgccgca ggcagctgga
    23761 cgatcgccag cgccagcttg atcaggagcg tcggcagcta gaagatgaag agcggagaat
    23821 ggaagatgag tatgggcgat gaggttgggt atggagcagg catcgcccct caccccaatg
    23881 gggagaggga gaaaaccagc gcaatattca atatcgagca caatcggtcc cctcgcccct
    23941 ctggggagag ggttagggtg agggggccag cgcgcacatt ccctctcatc acgccacccc
    24001 aatctccatc ccatcaaatc ccgcctcaaa ccccgacggc agtgcgtttt ccatcagcca
    24061 cgcatcaaac tggtggctga tatgggtcag aatcacccgt ggcgagcgga taacctgatt
    24121 cagcgccagc acggtgttta aatcacagtg actacgtggc gcatcctcgc gcggcgggtg
    24181 gctgcaatca atcaccatta cctgcggctg attattgcgt aaaaatttca gcgttttttc
    24241 cggcaagcct gcggtgtcag acagccacgc cacccggctg tgtgccgttt ccagcagata
    24301 accgaaggtc agttttgagt ggttgagcgg caggggcgtg acctgtaacc cctgcaaatc
    24361 aaacaccaca aacggttcca ccgtgtggct gaaatcaagc aggcccggat gtttaaacag
    24421 atcgtcgcag ccctgttcat ccggcgggcc gtaaaccggg atcggatcgc ccacgcccca
    24481 gcgcagcggg aatagcccct gcacgtgatc catatggtag tgcgtcagca aaaactgctg
    24541 gaacgatccg ggcggccagc gatcggcgag atcgtgcagc ccggcgtcga tcagggtgat
    24601 tgcgtcgtta aacttcacta cgccgctgca tggctggcgg cgatactgcg gtgagcgccg
    24661 cgctctggcg caggctacgc actcgcagcc ccatgccgga acgccctgtg cgccgccggt
    24721 gccggtgagc gtgagggtca ggctcatgtt acagcgcctt ggtgaagcgg aagtggctct
    24781 gctcgtagcc ttcacgcagg taaaaacggt gcgcgtcgtg gcgcttcacg ttggtggaaa
    24841 gttcggtcat ttcggccccg gcctggcggg cttcttcttc tgcccacgcc agtaacttac
    24901 tgccgacgtt cagaccgcac gcctgcggca tcaccaccag ttcctgaatt tcgccgatcc
    24961 agttgacatg atgcagatga aactgcaaat gcaggccgat catgccgaca acttcgccat
    25021 ccagcagcgc cagatggtag cgcatgctcg ggtagcgcat gctcgggtag cgcatgctcg
    25081 ggtcgcgcag gttggcgtta aagcccacgc gaaacgcgtg gtggtcaaac tccgcctgtt
    25141 ttagctcaca aatcagcgcg taaaccgcgt cggtatcgta ctgcgtggcc gggcgaagct
    25201 cacaagcagg catggtgttt ctccttctga tgaatcagcg tcagcagtgt gtcgaccgac
    25261 tggcgcaggc tgccgtcatt gttgagcgta tggcaatcct gtggagtata gcgggcggcg
    25321 cgcgccagcc gggcgttaat ttcactggtg ttttcacggc cacggttttt caggcgctgg
    25381 cgcaggatct ccggcgaaac ctgtaaacag acgggcagca gcgccgattg atagcgcgcc
    25441 cgtgcctgcg gcaaatgcgc gcgtgaaccg ttgacgacta cgtcgaatcc agcgtgcagc
    25501 cagaggtcaa tctcgatgcc gacgccataa tacagaccgt tagcgtgcca gcttaaggcc
    25561 agcaggtttt gccccgcgcg ggtaaaaaac tcccgctcac tcagggcgat atggttttca
    25621 cttccggcgc tggcgtcgcg cgtgatgtag cgatgcgcca ccagtaactg cgtttgttcc
    25681 cgcaggcgga gttccgccag caggctgtct ttcccggagc cggacggccc cattaaccaa
    25741 atcagttttc ccatcatcag aacacccttt taccctgacg ccagacgtgg tcgatatgaa
    25801 tgtgattgcc ctggcgatgc gccagcacca ggtcagcgcg tttaccctcg ccaatcaccc
    25861 cgcgatcctg aagattcagc gcctgcgccg gatttttggt caccagcctc accgcctgcg
    25921 gtagcgtaaa gcggttgctc tcgtcatcgg cgacgcggaa cgccgcatcc agcaggctgg
    25981 cggggtagta gtcggaagag aggatatcca gcagaccaag ctgcgccagt tcactggccg
    26041 ccacattgcc ggagtgcgag ccgccacgca cgatatttgg cgcacccatc agcacgttca
    26101 tgccatgctt acgcgaggct tccgccgctt cgaacgtggt ggtaaattcg gcgatcacgc
    26161 tgccaagttg gtgggattcg gcaacgtggg cgtgcgtggc gtcatcgtgg ctggcgagcg
    26221 caatttgtcg cgcgcggcac agggcggcga tggattcgcg attcggctgc gaccagcgtg
    26281 cggcgagcgc caattgctct tcttcgtact gctgcatctg cgcatcggtg agggagtatt
    26341 tgccctgata atattcgcga tacttctcgc ggttagcgaa ctggcgctgg cccggtgagt
    26401 ggtccattag cgacaccagc gtcaccggtt cgcgctgaac cagtttttca aacagcggca
    26461 gtgtggtgtg atgcggcagc tcgcagcgca ggtgcagacg gtgctcggcg cggttgacgc
    26521 cgcgtttctg cgtctcttcg atggtgttga tcatcttctc cagattctcc agctgatcgc
    26581 cgccgtcgcg cacgtcgcca atcgccaccg cgttcagcac ggtggtgatg ccgcttgcca
    26641 ccatcagcgc gtcgtggctg ctcatcgccg aatgggcggg ccagtcgact ttcgggcgcg
    26701 gggtgaagaa tttatcgagg ttgtcggtat gcagctcaat caggccgggt agtagccagc
    26761 caccttcgcc gtccatcgct tctggcaggc ggctctggct ttcggcaaag gcgcggattt
    26821 cgccatcctg cacctccagc gaaccgtgca ccacctcgtt ttccagcacc agcttaacgt
    26881 tattgataat catgaagagg ctcccattgg gtgcaggcgg tcggcgacgt cattacgtac
    26941 agcttcgtca tggaagatgc cgacgatggc tgcgccacgg gctttggctt cgtgaatcag
    27001 ttccaccacc gcggcgctgt ttttggcgtc cagcgaggcg gtaggttcgt cgagcagcag
    27061 aatggggtag tcgacgataa agccgcgggc gatgttgacg cgctgctgtt cgccgccgga
    27121 gaaggtcgat ggtgccaggt gccacaggcg ttccggcacg ttcaggcggg tgagaagacg
    27181 cgcggcttta gcggcgcagg cttcacgcgg aacgccggta tcgagcagcg gctgcatcac
    27241 cacttccagt gctgagatac gcgggatgac gcgcagaaac tggctcaccc agccgactgt
    27301 ggttttgcgg atttccacca ctttgcgcgc tggcgcggtg accaggtcta cccactcgtc
    27361 accgtgtttg atctggattt gaccttcgtc gggtagatag ttggcgtaca gcgagcgcag
    27421 cagggttgat ttgccgctgc cggagtgacc gtggagcacc acgcattcgc ctgcgttgac
    27481 ggtgagcgag gcacgttgta agacgggcag gcgtacgccg ttttgctgat gcaggatgaa
    27541 ggttttactg acgttttgta cgttaatcat ttttcgcctc gtggttcggg tgtcggatgc
    27601 gacgctgacg cgtcttatcc gacctacggg gagcgcattt gtaggccgga taaggcgttt
    27661 acgccgcatc cggcaccggc tcaattctgc aaaaccgatg acaccagcag ttgtgtatac
    27721 ggatggtgcg ggtcgtcgag cacgcggtcg gttaacccac tctccaccac ttgcccctgc
    27781 ttcatcacca gcaaacggtc cgccagcagg cgggcgacgc ctaaatcgtg ggtgacaatc
    27841 actaccgcga ggttcagctc caccaccagg ccgcgcagca ggtcgagcag gcgtgcctgt
    27901 accgacacat ccagtccgcc ggtcggctcg tccataaaca ccagcttcgg atgcgtcacc
    27961 aggttgcggg cgatctgcaa acgttgctgc atgccgccgg aaaaggtggt cggcaggtcg
    28021 tcgatccggt tggcgggaat ttccacctct tccagccact tctgcgcggt ggcacgaata
    28081 tcgccgtaat gacgtgcccc ggtcgccatc agccgctcgc cgatattgcc gcctgccgac
    28141 acctggcggc gcaggccgtc gagcggatgc tgatgcacta cgccccattc ggtgcgcagc
    28201 aggcgacggc ggtcggcctc gctcattgcg tacagcgaac ggctctcata gtgaatctcc
    28261 ccctgctgcg gcgtcaggcg cgcggagatt gatttcagca gcgtggtctt tccggagccg
    28321 gactccccga caatgcccag cacttccccc ggccataaat caaaagagac atcgctaaag
    28381 cctttgcccg gcgcgtaaag gtgggtcagg ttattgaccg aaagtaacgg ttgattcatt
    28441 ggttttttgc ctcgctctgt tggcggcaat aatcggtatc ggagcagaca aacatgcggt
    28501 ttccggcgtc atccagcacc acttcatcaa gatagctgtg ggtcgatccg cagatggcgc
    28561 atggctcatc ccactgctga acggtgaacg ggtgatcgtc gaaatcgaga ctttccacgc
    28621 gggtaaacgg cggcaccgca tagatgcgct tctcgcgccc cgcgccgaac agttgcaggg
    28681 cgggcatcat gtccattttt gggttatcga atttcgggat cggcgacggg tccatcacgt
    28741 agcgcccgtt caccttcacc ggataggcgt aggtagtggc gatatgaccg aagcgggcga
    28801 tatcttcata cagtttcacc tgcatcacgc cgtactcttc cagcgcgtgc atggtgcggg
    28861 tttccgtttc gcgcggctcg ataaagcgca gcggttcggg gatcggcacc tggaagataa
    28921 tgatctgatc ttcggtcagc ggcgtttcgg ggatgcgatg gcgcgtctgg ataagcgtcg
    28981 catcgtccgt acgttcagtg gtgtttaccc cggtcacgcg cttaaagaag ttgcgaatcg
    29041 acacggcgtt ggtggtgtcg tctgcgccct ggtcaatcac cttcagcacg tcgctttcgc
    29101 caatcacgct ggcggtgagc tgaatgccgc cggttcccca gccgtagggc atcggcatct
    29161 cgcgcccgcc aaacggcacc tgataaccgg ggatcgccac cgcttttaag atggcgcggc
    29221 ggatcatgcg tttggtctgc tcgtcgaggt aggcaaagtt gtagccgctc agattagcca
    29281 tggttctgct cctgttgcag acgtttgagt agctccagtt cggcctggaa atcgacgtag
    29341 tgggggagtt tgaggtgcga gacaaagcct gcggcttcga cgttgtcggc atgtgccagc
    29401 acgaactctt catcctgcgc cgggcctgtc gcgtgctcgc cgtactccgg agcctgcaac
    29461 gcacggtcaa ccagcgccat cgctttgcgc tcgctcatgc cgaacaccag cccgtagccg
    29521 cgcgtgaagt gcggcggctc atccggcggg tcaataaaac cgttgaccat ttcacactcg
    29581 gtcatcagca gttcgccgac gttcaccgca aatcccagct cttccggcat aatcgacacg
    29641 tcgatgtaac cactgcggat ttcgcccgcg aacgggtgat tgcgtccgta accgcgctgg
    29701 gtagagtagg ccagcgccag caaatagcct tcgtcgccgc gcatcaactg ctgcaaacgg
    29761 gaagaacgtg aacaggggga aactggcgga gtgcgggtga tgtcatccgg ctgtgcgccg
    29821 ctatcctctt caaacttcgc cagcccctga cgcgccagca ggctgaaaac gtgcggcgac
    29881 ggggattgtt cgctgtctgc ggtggtcagc gtcggcgctt cgccgtttgc cagcagggtg
    29941 aaatcgagca ggcgatgggt gtagtcgtag gttgggccaa gcaactgacc gccgggaata
    30001 tctttataaa ccgctgaaat acgccgttcg agacgcatct ccgtggtgtc gagcggctcg
    30061 cttaccgcca gcttcgccag cgtggtgcgg taggcgcgta gcaggaaaat cgcttcgacg
    30121 ttatcgccgc tggcctgttt cagcgccagc gccgccagtt cgcggtcggc aatgccgcct
    30181 tcggtcatca cgcgatctac tgcaagatta agctgctgtt caatctgggc gacgctcagt
    30241 tcaggcaaat cggtattgcc ccggcgtcgg ctctcttgca gggcgtgggc ggcgtcgatc
    30301 tccttctcgc cccctttcac ggcaacgtac atcagcacac ctccacatga gtggttcgcg
    30361 ggatagccag cagccgctcg ccgcaggtca ggatcaggtc gatgccgagc gggaacgggt
    30421 gcgggcgctc ggtgagttcg tgcagaatgc actccggcag ctgcggagcg atcattcgtt
    30481 cttcggcaat acccgcgcca gtaaggcgca gcatgcgccc gccgctcagg ctgacgacct
    30541 gtaaaatcag cgtcgcgccc gcttccggcg caacagcggt gccgctggaa agagcgttga
    30601 gctgttcgct ggaaatcgcc tcatccgtta ccgcgaaggt cgccagttcc gactgattga
    30661 ccagcggcgc gttggtatga aaacgcaggc tctggctgac gatatcgtta cttaatggcg
    30721 cggaaagcca caccggcgtg tcgttatcag ccagcgtcag cagcacgctg gtggtggcga
    30781 tattcagcgg ctgccagccg cgtttgagct gatgcagggc gacaatcacg cccggctcgc
    30841 tcatggcctt taacaggcga cgaaaactgt gctgggcatc ctgcacagga agcataaaag
    30901 cggtttccag ggtcatgcgt tgtctccgcg aaccatcgta aagaagtcga cccagctggc
    30961 gttcacttcg gcctggcgtg cggcaatgcg tgccatacgg tcagcgtcca gtggggcaat
    31021 aagggtttct gataagtttt gaaaatggcg agattgctgc atcaacgcgt caatcagcgc
    31081 gcagcgttcg gcgtgctgtt tgtcgcgccc cagcacccag ctgtagccga gggggccgtc
    31141 agtcaggcgt acggcggcgc gggtcagcgt ggcgtcgccg gcaaaaaagc gttcgccggt
    31201 accgcccatt cgcggctgaa tctgtaccag gccggtttcg gcggtgcgga tcacttcata
    31261 gttggcggtg atgttcagcg cgttcagccg gttggctgtg cgccagcatg gacatccagt
    31321 gctggcgggt cgcgatatct gcgtgcattc agtgctccat agtgaattca atcatgtcgg
    31381 cgcgcgtcag gctgacggag tactccgccg ggctgctttc accgtcacgg tggttaaggg
    31441 tgcgcacgca cagcagcggc gacatattcg ggatttcaag acgctggcac tctttggcct
    31501 gggcgcggcg ggcgctgatc cgcgtctggc tgcggcgcag cgcaatcccg gtttgctcgc
    31561 gcagaaaatc gtgcagcgag ccgctgtcga agcgttgcag cgtcggccaa agggtgaggt
    31621 ccgcaaagta gtggtcgatt aaacagagcg cgatgccgtt gacccggcgc agggtgcgca
    31681 ggtggatgac gttctccccc tcggttatcc ccagcgcgtc agcgacgtga ccagatgccg
    31741 ggcgcagcac cgacagcagt ttttcgctgg tgggatggct gccctgatcc agcaggttct
    31801 ggctaaaacg cgcctgggcg ttgagcgggt aatcgaacgg gcgcatcagc accagcacgc
    31861 cgacgccctg acggcgctgt acccagcctt tttccaccag ttggtcgatg gcgcggcgca
    31921 gggtgtggcg atttacctca aagcgcgccg ccagctgctg ctcggcggga agatagtcgc
    31981 cgcagcgata gtgttgacga agctcctgct caagttttgc ggctatctcc tgatagcgtg
    32041 ttgggtagct ggtcggatgt gtagacaagt gcatagatat caatgcctcg cttatcagat
    32101 aaagtgctta cgcaaccgtt gagagaggaa atccagcagg ctgaccgtga cgatgataag
    32161 caccatcagg gcgcaggttt gttggaactg aaaaccgcga atcgcttccc acagggtgac
    32221 gccgatcccg cctgcaccga ccatgccgac gacggtcgcc gagcggacgt tggattcgaa
    32281 gcgatagagg gagtaggaga tcagcagcgg cattacctgt ggcagcacgc cgtaaaggat
    32341 ctcttccagc ttgttggcgc cggtggcgcg aatgccttct accgggccgg gttcgatcgc
    32401 ttccaccgct tcggaaagca gcttggagag cacgccggtg gtgtggataa atagcgccag
    32461 cacgccagcg aacgggccga gaccgacggc gaccacgaac agcatggcga agaccatttc
    32521 gttaatggcg cggcaggcat ccatcaggcg gcgaacgggc tggtaaaccc accacggcac
    32581 caggttttcg gcgctcatca gaccgaaggg gatggagaga accactgcca gcgcggtgcc
    32641 ccagacggcg atttgcagcg tgacggccat ttcggtgagg taatcctgcc actggctgaa
    32701 atcgggcggg aagaagtcgg caacgaaggt tgccatgttg ccgccgtctt taatcagcgt
    32761 aagcggggcc atttccgcgc cctgccacga gacgaccagc acggcgagaa caacggccca
    32821 gctcagaagc gagaaccagc tacgcttggg tggggcgatg gtgatggttt gcatgtttgg
    32881 ctccgtttaa tgttttctcc ctctccctgt gggagagggc cgggatgagg gcatcagcgt
    32941 gcgcatttcc ccctcaccct aaccctctcc ctcaagggag aggggaccga tcgagcacat
    33001 tactgcaccg ctttactcac cgaactcatc gcgcttaacg cgttgttcag gcggtccaag
    33061 tcatccagct gcgcctgaat ctcggtggtt ttcgccagct tgtcctgctc attcagtcct
    33121 ttattgcttt ttacgccctg catctcttta aacagtgcga gctggcgaat cggcaccagt
    33181 tgcaggtcgc tggaggcgcg gaacggggcc cagcccaggc gttccagcac cgctttctct
    33241 tccggcgttt ttccgtagtt cataaagaag tcgtagatct tgtctttggt ggtttcggag
    33301 agattcttgc gccagacgat cggatcgcct gggatcagcg gcgacttcca gatcaccttc
    33361 agttctttca gcttctctgg cgcggaggtt ttcagcttgt cgaggttttc ggtgttgttg
    33421 gtggcaacat ccacctgctt gttggcgacg gccagcgcgt tggtttcatg cccagcgttg
    33481 acggtgcgct taaagtcgct ggcggagata ttgtttttgg caaagacgta gtaaccgggg
    33541 acgaggaagc cagaggtgga gttaggatcg ccattgccga aggtgagatc tttccgcttc
    33601 gccagcagat cgttcaggtt gttgatcgga ctatctttgt tgacgatcaa cacgctccag
    33661 taacctggcg atccatccgc cgcgaccgtc tgggcgaaga cctggccgtt ggcgcgatcc
    33721 accgcttcca ttgccgacag gttgccgtac caggcgatat ccactttatt gaagcgcatt
    33781 ccctggataa tgcctgcgta gtctggggca aagaaggcgt tcaccttcac gcccagcttc
    33841 ttctccatat cctgtaagaa tggcgtccat tgcggtttca ggttttgctg tgattccgtt
    33901 gaaataatgc cgaaattcag cgccttttcc tgctcttcgg cgtgtgccgg gcttaacagg
    33961 gtgctgaggc tgaacatgct ggtgaaggcc agcgaggcaa ttatcttagc gttcattcgt
    34021 tttcctcaat gatggggatg tcaggcagct ttcgcgttct cttcgatgcg attaatgctg
    34081 cggtagagat ggtcaaaacg ttcgttatca aactgttggc tgctgccgtc gtagaaaacg
    34141 tgcccctggc gcagggcgac gatgcgttcg cagtagcgca gggcgtaatc cacctgatgc
    34201 agcgtgacga ctacggtgat gccgtcgttc tgattgatgt cgcgcagggt gtccatcacg
    34261 atgcgggcgg attccgggtc cagcgaggcg atgggttcat cggccagaat caccttcgcc
    34321 tgctgcatca gcgcgcgggc aatcgccaca cgctgctgct gtccgccgga gagggtggaa
    34381 acgcgttgat gggcaaaatg caccatgcca acgcgggtca gcgcctgtaa cgcgcgttgt
    34441 ttctgctcgc gggtgaacca gctaaaacag gtgcgccaga acggcgtgct gccgagcgcg
    34501 ccaatcagca cgttctccag tacgctcagg cgattcacca ggttgaattg ttggaagatg
    34561 tagccggtat gggcgcggct tttgcggata tcgcgcgcca gacggccttc gcgctggact
    34621 gtgcggccca gcagctcgat atggctgccg gcggatttat cgccggtaat caaaccgctt
    34681 aagtgacgta aaagtgtgga tttgccggaa cccgacggcc caagcagagc caccatttca
    34741 ctgtgatgaa tgttcagatc aaccgcatgc agcgcttgat gctgattgaa ggttttggcg
    34801 agcttctcga cacggataat cgtttgcatg atgcagcctc cctaaaaaag tggctccatc
    34861 gtggcggatt attgtgacga tttggttaat taaaagtgac taacagatga agagttaacg
    34921 ggaattcgat gacagtgcgg gagggcgggc ctcccggcgg gttattgcgt tggttgttgt
    34981 ttgacgacat taatcatcca cggtacgcca aatttatcgg tgactttgcc gaagccatgc
    35041 gcccagaaag tttcctgcca ggccatttca atttttccgt tagcggcaag gttatcaaac
    35101 cagcgttttc cttcttcgac ctgttgcgaa tcgagcacca gcgtaaagcc ggagtagctg
    35161 gcttttcctg acggtatagc atcgctcatc atgatgtcgc ttccggcaat gcgcacgttg
    35221 gcgtgggcga tggcggtatc gggaaattgc attccggaag ggcagttctc ggcgctgtcc
    35281 tgcgctgatt ttggcatttc gccgaagctg attttataga gcagttccgc gcccaacgta
    35341 cgttgataat aggcaatcgc gtcggaacag ttaccggcaa aagagaggta gggacttaac
    35401 ggcatgatgg tgacctcagt taagagaagc cagttaagtg tagttcgaaa tttatacaga
    35461 tgagagggaa gcctgataag gcgcggtcag cgtcgcatca ggcagtcggc atcgttgccg
    35521 gatgtggcgc gagcgcctta tccggcttac cgatcacaat acaatcagtt ctttttcaca
    35581 aactcagatt tcagtttcat cggaccaaaa ccgtcgattt tgcaatcgat gttatggtcg
    35641 ccttcaacca ggcggatgtt tttcactttg gtgccaattt tcagcatcga agagctacct
    35701 ttcaccttca gatctttaat gatggtaacg ctgtcgccgt cagccagcag attgccgtta
    35761 gcatctttaa cgatcagctc gtcgctttcc tgtgcaggtt ctgtgtcgtt ccattcgtag
    35821 gcacattccg ggcagatgta catgccgtta tcttcgtaag tgtattcgga gttgcatttt
    35881 gggcagtgtg gtaatgacat gtggatttcc tcaaaagtca gcagggcaaa agcgccaaaa
    35941 aacggcagat tgccgaaaaa ggccgcaatt atacacaaaa tccctgagtt tgtcgggggt
    36001 actgctgatt ttagacgacg atcgttttca tttgaaagga aacgcaaaaa gaaaataatc
    36061 tttttgcctc tatttattat ttaagataat gttaatgctc tacctgttct ttttttattc
    36121 acatgatatt tttatctttc tgacgtttta aacgcatcag aagacgttat ttattctgat
    36181 aaaaaacaac acactaaata taaaat
//
LOCUS       DAZPEB010000017        35132 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZPEB010000017 DAZPEB010000000
VERSION     DAZPEB010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35132
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.017"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            32..1270
                     /gene="dsdA"
                     /locus_tag="ACHURJ_000781"
                     /pseudo
     CDS             32..1270
                     /gene="dsdA"
                     /locus_tag="ACHURJ_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
     gene            complement(1378..2916)
                     /gene="emrY"
                     /locus_tag="ACHURJ_000782"
     CDS             complement(1378..2916)
                     /gene="emrY"
                     /locus_tag="ACHURJ_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFR1279841.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(2916..4079)
                     /gene="emrK"
                     /locus_tag="ACHURJ_000783"
     CDS             complement(2916..4079)
                     /gene="emrK"
                     /locus_tag="ACHURJ_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFR1279842.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYVYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKNEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            4495..5109
                     /gene="evgA"
                     /locus_tag="ACHURJ_000784"
     CDS             4495..5109
                     /gene="evgA"
                     /locus_tag="ACHURJ_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFR1279843.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            5114..8707
                     /gene="evgS"
                     /locus_tag="ACHURJ_000785"
     CDS             5114..8707
                     /gene="evgS"
                     /locus_tag="ACHURJ_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFR1279844.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVVLNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDIIPGAIYSEDRENNVSFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILITFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(8763..9908)
                     /gene="yfdE"
                     /locus_tag="ACHURJ_000786"
     CDS             complement(8763..9908)
                     /gene="yfdE"
                     /locus_tag="ACHURJ_000786"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFR1279845.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDYDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWETLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFNTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAIKLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(9982..10926)
                     /gene="yfdV"
                     /locus_tag="ACHURJ_000787"
     CDS             complement(9982..10926)
                     /gene="yfdV"
                     /locus_tag="ACHURJ_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFR1279846.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIVYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(10996..12690)
                     /gene="oxc"
                     /locus_tag="ACHURJ_000788"
     CDS             complement(10996..12690)
                     /gene="oxc"
                     /locus_tag="ACHURJ_000788"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFR1279847.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVIGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSSVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(12744..13994)
                     /gene="frc"
                     /locus_tag="ACHURJ_000789"
     CDS             complement(12744..13994)
                     /gene="frc"
                     /locus_tag="ACHURJ_000789"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFR1279848.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(14507..15148)
                     /locus_tag="ACHURJ_000790"
     CDS             complement(14507..15148)
                     /locus_tag="ACHURJ_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFR1279849.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQQVQQTQK
                     TAAAERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPG
                     KKTNLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVG
                     VMENQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            15444..15719
                     /gene="ypdI"
                     /locus_tag="ACHURJ_000791"
     CDS             15444..15719
                     /gene="ypdI"
                     /locus_tag="ACHURJ_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HFR1279850.1"
                     /translation="MKVNLILFSLFLLVYIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(15796..16038)
                     /locus_tag="ACHURJ_000792"
     CDS             complement(15796..16038)
                     /locus_tag="ACHURJ_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFR1279851.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTEIFMFLAPACGLMIRFMVGYGRR"
     gene            16391..17311
                     /gene="lpxP"
                     /locus_tag="ACHURJ_000793"
     CDS             16391..17311
                     /gene="lpxP"
                     /locus_tag="ACHURJ_000793"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFR1279852.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHCRFKTRPVGESSLYI"
     gene            17667..17738
                     /gene="ypdK"
                     /locus_tag="ACHURJ_000794"
     CDS             17667..17738
                     /gene="ypdK"
                     /locus_tag="ACHURJ_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFR1279853.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(17803..19041)
                     /gene="alaC"
                     /locus_tag="ACHURJ_000795"
     CDS             complement(17803..19041)
                     /gene="alaC"
                     /locus_tag="ACHURJ_000795"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFR1279854.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRQDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWIVEMPKASMYVWAKI
                     PEPYAAMGSLVFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            19418..21115
                     /gene="ypdA"
                     /locus_tag="ACHURJ_000796"
     CDS             19418..21115
                     /gene="ypdA"
                     /locus_tag="ACHURJ_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFR1279855.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            21130..21864
                     /gene="ypdB"
                     /locus_tag="ACHURJ_000797"
     CDS             21130..21864
                     /gene="ypdB"
                     /locus_tag="ACHURJ_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFR1279856.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            21877..22734
                     /locus_tag="ACHURJ_000798"
     CDS             21877..22734
                     /locus_tag="ACHURJ_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR1279857.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNSQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYTQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(22737..25232)
                     /gene="ptsP"
                     /locus_tag="ACHURJ_000799"
     CDS             complement(22737..25232)
                     /gene="ptsP"
                     /locus_tag="ACHURJ_000799"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFR1279858.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQAQRVLEEYIQVRFIDSDSIQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASSVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRESDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPIDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(25257..26294)
                     /gene="ypdE"
                     /locus_tag="ACHURJ_000800"
     CDS             complement(25257..26294)
                     /gene="ypdE"
                     /locus_tag="ACHURJ_000800"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFR1279859.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGTRSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHSAELPAEVWLVASSSEEVGVRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQICNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMQQLLSALIKRLT
                     RETVVQLTDFR"
     gene            complement(26294..27379)
                     /gene="ypdF"
                     /locus_tag="ACHURJ_000801"
     CDS             complement(26294..27379)
                     /gene="ypdF"
                     /locus_tag="ACHURJ_000801"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFR1279860.1"
                     /translation="MTLLASLRDWLKEQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSSYFVEVEARAQGYQLHLLDATNTLTTIVNQIIADEQLQTLGFEGQQVSW
                     ETAHRWKSELNAKLVSATPDVLRQIKTPEEVEIIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGVLPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFDVYQIVLQAQLAAISAIRPGVRCQQIDDAARRV
                     ITEAGFGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(27394..28641)
                     /locus_tag="ACHURJ_000802"
     CDS             complement(27394..28641)
                     /locus_tag="ACHURJ_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFR1279861.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGVIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(28663..28989)
                     /locus_tag="ACHURJ_000803"
     CDS             complement(28663..28989)
                     /locus_tag="ACHURJ_000803"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFR1279862.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(29208..30173)
                     /gene="glk"
                     /locus_tag="ACHURJ_000804"
     CDS             complement(29208..30173)
                     /gene="glk"
                     /locus_tag="ACHURJ_000804"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFR1279863.1"
                     /translation="MIKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            30377..31633
                     /locus_tag="ACHURJ_000805"
     CDS             30377..31633
                     /locus_tag="ACHURJ_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFR1279864.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTINIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            31748..32074
                     /gene="ypeC"
                     /locus_tag="ACHURJ_000806"
     CDS             31748..32074
                     /gene="ypeC"
                     /locus_tag="ACHURJ_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFR1279865.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(32215..33453)
                     /locus_tag="ACHURJ_000807"
     CDS             complement(32215..33453)
                     /locus_tag="ACHURJ_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFR1279866.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            33789..34991
                     /gene="nupC"
                     /locus_tag="ACHURJ_000808"
     CDS             33789..34991
                     /gene="nupC"
                     /locus_tag="ACHURJ_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFR1279867.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(35038..>35132)
                     /locus_tag="ACHURJ_000809"
     CDS             complement(35038..>35132)
                     /locus_tag="ACHURJ_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1279868.1"
                     /translation="PTRRGRALPRVVKERPWKYPTAPKKSQSVA"
BASE COUNT         9250 a         8728 c         8112 g         9042 t
ORIGIN      
        1 gtcacggcga actgcctttc aaggttgaag tggcacgacc tcattggctg aaggtttacc
       61 ttatgttggc ctgaccgaac aggatgttca ggacgcccat gcgcgcttat cccgttttgc
      121 accctatctg gcaaaagcat ttgctgaaac tgctgccact ggggggatta ttgaatcaga
      181 actggttgcc attccagcta tgcaaaaacg gctggaaaaa gaatatcagc aaccgatcag
      241 cgggcaactg ttactgaaaa aagatagcca tttgcccatt tccagctcca taaaagcacg
      301 cggcgggatt tatgaagtcc tggcacacgc agaaaaactg gctctggaag cggggttgct
      361 gacgcttgat gatgactaca gcaaactgct ttctccggag tttaaacagt tctttagcca
      421 atacagcatt gctgtgggct caaccggaaa tctggggtta tcaatcggca ttatgagcgc
      481 ccgcattggc tttaaggtga cagttcatat gtctgctgat gcccgggcat ggaaaaaagc
      541 gaaactgcgc agccatggcg ttacggtcgt ggaatatgag caagattatg gtgttgccgt
      601 cgaggaagga cgtaaagcag cgcagtctga cccgaactgt ttctttattg atgacgaaaa
      661 ttcccgcacg ttgttccttg ggtattccgt cgctggccag cgtcttaaag cgcaatttgc
      721 ccagcaaggc cgtatcgtcg atgctgataa ccctctgttt gtctatctgc cgtgtggtgt
      781 tggcggtggt cctggtggcg tcgcattcgg gcttaaactg gcgtttggcg atcatgttca
      841 ctgctttttt gccgaaccaa cgcactcccc ttgtatgttg ttaggcgtcc atacaggatt
      901 acacgatcag atttctgttc aggatattgg tatcgacaac cttaccgcag cggatggcct
      961 tgcagttggt cgcgcatcag gctttgtcgg gcgggcaatg gtgcgtctgc tggatggctt
     1021 ctataccctt agcgatcaaa ccatgtatga catgcttggc tggctggcgc aggaagaagg
     1081 tattcgtctt gaaccttcgg cactggcggg tatggccgga cctcagcgcg tgtgtgcatc
     1141 agtaagttac caacagatgc acggtttcag cgcagaacaa ctgcgtaata ccactcatct
     1201 ggtgtgggcg acgggaggtg gaatggtgcc ggaagaagag atgaatcaat atctggcaaa
     1261 aggccgttaa taacgtttca acgcagcatc gcaatccttt ccctgggtga gcgatgctgc
     1321 cgatggcgca gacttaagat ccccggtctt acccgctata accccctcct tttatcatca
     1381 cccaacgcct ttcgctgtaa acggcggttt cgcaaaccaa accaaaaccg taagtaagat
     1441 aaaagcaata gccgccatac ggaaaatctc atttgcagaa atagaaagtg actgctgggt
     1501 tatttcatta ttaatttcat taagaactcc tgaaagcgaa ccataatatt tatccataat
     1561 ttgtgacgat gaattaaaca cggggttaaa ttgatcgatg gttgctgtca actgactatg
     1621 gtgtaacgat tcgcgtcgtc cccacagcgt cattgtcaac gacgtaccaa ctgatcctga
     1681 caaggtacga aaaaaattac tcatactcga ggcattggca aatttattat ctggcaagcc
     1741 tgaaaacgaa atcgttgtta agggtaaaaa gaaacaggca acggcgaatc cctgaaaaaa
     1801 ctgcggcaaa atgatgcctg taaaatcaat cgttggcata aatgtcacag aacgccagta
     1861 atagcaaacc gcatacatca aaaaactaaa tgtcactaac aaccgcatgt ctattttgtt
     1921 gccataacgt cctatcaaag gtgaaattaa tagtggcatg atgccgatgg gcgcataagc
     1981 aagtccggcc catatcgcat tataccccat cgtttcctgg agtaactgcg gcataaggac
     2041 gatcgctcca gagtaaaata aatacgcgca tgtgatactc acaataccaa tggtgaagtt
     2101 acgggactta aacaaactga gatcaagaat cgggttctct gaggtcgact cccaaatgac
     2161 taaagagatc agagaaataa ctgatactac tgttaatatt attattgtac tcgagttgaa
     2221 ccaatccaga tcgcgccctt tatcaagcat aatttgcaag ccaccaacac cgagcactaa
     2281 cagggtcagt cctggtagat tcattttgac cggtgaagtc tcagtttctc ttcctttaag
     2341 taaggttaag cataatgtca ggacgataat ccccataggg acattgatta aaaatatcca
     2401 accccagcta aagttatcac aaatataacc gcccaatatc ggcccacata tcggagcgat
     2461 aatcacggtc attgaccata atgccagagc aaatgttctt ttttctggcg gataattcct
     2521 taataataaa ctctgtgaca gtggaattaa cggccccgcc attaacccct gaacgactct
     2581 aaaaaatatc agcacatcaa gattggtcga taggctacac attaatgaag acagcgaaaa
     2641 aaaggttact gaaagtaaaa ataatcttaa ttcgcctatt ctttgtgcca acctgccagt
     2701 aacagggatc gcaatggcat ttgctacacc aaacgaggtg ataacccagg tgccttcgtc
     2761 tgttgatgct cccagaaagc cagatattgt cggtattgcg acgttagaaa tagtggaatc
     2821 caacatttgc ataaatgtcg ctaatgacaa tgcaatagtg acgcaccata acgtcccacc
     2881 ggttaatggt gccggagttg atttagtgat tgccattaaa gttgtccatt atgcgaaata
     2941 atgttgctaa tttctttttc tatcggactg gtatcgataa ctaaagcctt actggtataa
     3001 gccggcatgg aggtcacggt tgaagccagc tcaggcatct cggcaatgtc ttcgttcttc
     3061 gtatcaatag ttgctgtcat cgataaacca atacgcaagg gatgttccat gagttctttt
     3121 ggatcaagag aaacttcaac cggtacacgc tgaacgattt tgatccagtt ccctgtcgca
     3181 ttttgtgcag gtaataagga gaacgcattg ccggttccca tattgatccc tgtcacccga
     3241 ccatgaaaca caacattttc accataaaga tcgctgataa tattgaccga ttgaccaatc
     3301 cgcacatccg tgagttgtgt ttctttaaag ttggcattaa cccacatttg acgtgccggt
     3361 acgacagcca ttaacgattg tccggggctc actgtttcgc cgacctgaac acttctctgg
     3421 gcaatatagc cggtaaccgg actcttaata tccgtacgtt taagcgccaa ccaggcttct
     3481 ttagttgcat ccgccgcttc aacgacttgt ggctgacggt ttaatggtgt gttcattact
     3541 aaagctttat tcgctttata agcctggata gcggcattca atgccgcttt gctacttatt
     3601 aacgtatctt tggtatgctc cagcgtttct tttgaaataa ccccctgctt cgctaacggc
     3661 actcgacggt tataatcttc taaagattgt tgatactgaa tacgtgctga agcgacttcg
     3721 gcactgtatt gtttatcctg taagtatagt ttattcgttt gccgaacaat atttgccaga
     3781 ttatttttag ctttattgag tgcgatagtg gcatcagttt tatccagtga aactaaaatg
     3841 tcaccttgtc gaacgtagtt cgtatcttta tgattaacga cagtgacact acctgagact
     3901 tgtgcagaaa ttggatctgc attccccgtg acataggcgt catctgtact aatcatgtct
     3961 tctaattcca ttgaccaata gacataggca cctgaaaacg caataaataa aactaccgcc
     4021 aataaagaaa agtattttct tctgttagaa tgttttttat ttgaattaat ctgttccact
     4081 attatctctc atttctcata gatgaaattt atgaattgaa tgaaaagagc attcattctt
     4141 catatcaaac acagtgcatg tgttgcacat tatcccagga acgttggagg cctaatttaa
     4201 atgaggtgca aaaaaaacaa cagaggacta aaccgtggct tttgcaatac aattcttacg
     4261 cctgtaggat tagtaagaag acttatagtg ctaacttgaa actataaatc atcggtacaa
     4321 cccctgattt tattgttgac atttcattta tgccgactat ttatatggta tacttgtcga
     4381 attatcttaa aggaagctca gattttctta tttttattga gaaaatgaga tgacgcctta
     4441 tgtctgtatt actacaggga gaagggagat gcttcattgc aaagggaata atctatgaac
     4501 gcaataatta ttgatgacca tcctcttgct atcgcagcaa ttcgtaattt attgatcaaa
     4561 aacgatattg aaatcttagc ggagttgact gaagggggaa gcgccgttca gcgggtggaa
     4621 acacttaagc ctgatatcgt catcattgat gtcgatatcc ccggagttaa cggtatccag
     4681 gtgttagaaa cgctgaggaa gcgccaatat agcggaatta ttattattgt ctccgctaaa
     4741 aatgaccatt tttacgggaa acattgtgct gatgctggcg ctaatggatt cgtgagtaaa
     4801 aaagaaggca tgaacaatat cattgcggct attgaagctg caaaaaatgg ctactgctat
     4861 ttccccttct ctctcaaccg gtttgttgga agtttaacgt ccgaccagca aaaactcgac
     4921 tccttatcga aacaagaaat tagtgtcatg cggtatattc ttgatggcaa ggataataat
     4981 gacattgctg aaaaaatgtt catcagcaac aaaactgtca gcacttataa aagtcgcctg
     5041 atggaaaaat tagaatgtaa atcactgatg gatctttaca cattcgcgca acgtaacaaa
     5101 atcggctaat cacatgaagt ttttacccta tttttttctt ctctgttgtg gtctttggtc
     5161 gaccataagt ttcgcagacg aagattacat cgaatatcgt ggcatcagta gtaacaaccg
     5221 tgtcacactt gatccagtac gtctgagcaa caaggaatta cgttggttag cgagcaaaaa
     5281 aaatcttgtg attgcagtac acaagtccca aacggctacc ttgttgcata ccgattcgca
     5341 gcaacgggtt cgtggtatta atgctgatta tttaaatctt ttaaaaagag cgttaaatat
     5401 caaattaaca ctccgggaat acgccgatca tcaaaaagca atggacgcgc tggaagaagg
     5461 tgaagtcgat atagtgttat cacatttagt tgcttcgccg cctcttaatg atgacattgc
     5521 tgcaaccaaa ccactgataa ttacctttcc ggcgctcgtc accacccttc acgattcaat
     5581 gcgaccgctt acctcatcaa aaccagtaaa tattgctcga gtagcaaatt atccccctga
     5641 cgaggtaatt catcaatcat ttccaaaagc aacaattatc tcttttacaa atttatatca
     5701 ggcattagca tccgtctcag ccggacagaa tgattacttt attggtagta acatcattac
     5761 cagcagtatg atttcccgct atttcactca ctccttaaat gtagtgaaat attataactc
     5821 accgcgtcaa tataattttt tcttgaccag aaaagaatct gtcgttctta atgaagtact
     5881 caatcgattt gttgatgctt taacaaatga agttcgctat gaagtttcac aaaattggct
     5941 tgatacagga aacctggcct ttctgaacaa accattagaa ctcactgaac atgaaaaaca
     6001 gtggattaag caacatcccg atttaaaggt gctggaaaat ccttactctc ctccctattc
     6061 tatgacggat gaaaatggct cggttcgggg cgttatgggg gatattctta atattattac
     6121 cttgcaaaca ggtttaaatt tttctccgat caccgtttca cacaatatcc atgccggaac
     6181 acagcttaac cccggtggat gggatataat acctggcgct atttatagtg aagatcgaga
     6241 aaataatgtt tcatttgcag aagctttcat aacaacgcca tacgtttttg tcatgcaaaa
     6301 agcacctgac agtgaacaaa cattaaaaaa aggaatgaaa gttgccattc catattatta
     6361 tgagctgcat tcgcaattaa aagagatgta tccggaggtt gagtggataa aagtcgataa
     6421 cgccagcgct gcatttcaca aggtcaagga aggtgaactt gatgctctgg tcgcgacaca
     6481 gctaaattcg cgttacatga tcgatcatta ctatcctaat gaactgtatc attttcttat
     6541 tcccggcgtt ccgaatgcat cgctttcgtt cgcttttcct cgcggagaac cggaacttaa
     6601 ggatattatt aataaagcac tgaatgcaat tcccccaagc gaagttctgc gcctgacgga
     6661 aaaatggatt aaaatgccca atgtgaccat tgacacatgg gacctctata gcgagcaatt
     6721 ttatattgtt acgacattat ccgttttatt agttggcagt agccttttat ggggattcta
     6781 cctgttacgc tcagttcgtc gtcgtaaagt cattcagggt gatttagaaa accaaatatc
     6841 attccgaaaa gcactctcgg attccttacc aaatccaact tatgttgtaa actggcaagg
     6901 taatgtcatt agtcataata gtgcttttga acattatttc actgcggatt actacaaaaa
     6961 tgcaatgtta ccattagaaa acagtgactc accctttaaa gatgtttttt ctaatgcgca
     7021 tgaagtcaca gcagaaacga aagaaaatcg aacaatatac acacaggtat ttgaaattga
     7081 taatggcatc gagaaaagat gcattaatca ctggcataca ttatgcaatc tgccagccag
     7141 cgatcatgct gtttatattt gtggttggca agatattacc gagacgcgtg atttaattca
     7201 tgcactcgaa gtagagagaa ataaagcgat caatgcaact gtcgcaaaaa gtcagtttct
     7261 ggcaacaatg agtcacgaaa taagaacacc aataagctcc attatgggct tcctggaact
     7321 tctgtcgggt tctggtctta gcaaggagca acgggtggag gcgatttcac ttgcctacgc
     7381 caccggacaa tcactcctcg gcttaattgg tgaaatcctt gatgtcgaca aaattgaatc
     7441 gggtaactat caacttcaac cacaatgggt cgatatccct actttagtcc agaacacttg
     7501 tcactctttc ggtgcgattg ctgcaagcaa atcgatcgca ttaagttgca gcagtacatt
     7561 tcctgatcat tacctggtta agatcgaccc tcaggcgttt aagcaggtct tatcaaattt
     7621 actgagcaat gctctcaaat ttaccaccga gggggcagta aaaattacga cctccctggt
     7681 tcacattgat gacaaccacg ctgttatcaa aatgacgatt atggattctg gaagtggatt
     7741 atcgcaggaa gaacaacaac aactctttaa acgctacagc caaacaagtg caggtcgcca
     7801 gcaaacagga tctggcttag gcttaatgat ctgcaaagaa ttaattaaaa atatgcaggg
     7861 cgatttgtca ttagaaagtc atccaggcat aggaacaaca tttacgatca caatcccggt
     7921 agaaattagc cagcaagtgg cgactgtcga ggcaaaagca gaacacccca tcacactacc
     7981 tgaaaagttg agcatattaa tcgcggatga tcatccgacc aacaggctat tactcaaacg
     8041 ccagctaaat ctattaggat atgatgttga tgaagccact gatggtgtgc aagcgctaca
     8101 caaagtcagt atgcaacatt atgatctgct tattactgac gttaatatgc cgaatatgga
     8161 tggttttgag ttgactcgca aactccgtga gcaaaattct tccttaccca tctgggggct
     8221 tacagccaac gcacaggcta acgaacgtga aaaagggtta agttgcggca tgaacttatg
     8281 tttgttcaaa ccgttgaccc tggatgtact gaaaacacat ttaagtcagt tacaccaggt
     8341 tgcgcatatt gcacctcagt atcgccacct tgatatcgaa gccctgaaaa ataatacggc
     8401 gaacgatcta caactgatgc aggagattct catcactttc cagcatgaaa cgcataaaga
     8461 tctacccgct gcgtttcaag cactagaagc tggcgataac agaactttcc atcagtgtat
     8521 tcatcgcatc cacggtgcgg ctaacatcct gaatttgcaa aagttgatta atattagcca
     8581 tcagttagaa ataacacctg tttcagatga cagtaagcct gaaattcttc agttgctgaa
     8641 ctctgtaaaa gaacacattg cagagctgga ccaggagatt gctgttttct gtcagaaaaa
     8701 tgactaaata gcggctccca caatgttcaa atgtgggagc tatttaccag cacatcttac
     8761 atttatgatg agaactcctg gcgaatttgt tccccatgct ggtcgagcgt tgccgctccc
     8821 ggcataacat gcgggtccgc gcagccgctg atttttatcg gattacccgg catcattatt
     8881 cccccggctt caatcaacat atttctcgcc tgagtttgtg gcaatttaat ggcctcagcc
     8941 acacttaata acggcgcgac gggtacacca acttcatgta ttctggctaa ccaaacttca
     9001 gctgcctgcg tttttaacgt ccgctcaata tattgtttaa gaatagcctg gttttgtacg
     9061 cgtaaaatat tgctgctaaa tcggggatca ttaaccagtt ccgtaagctc cagtgcctgg
     9121 cataacgcag aaaaaagctt gtcattacca caacaaatcg taatcggctt atcctgagta
     9181 ttgaaaacat caaaaggtgc catgtaggga tggcgatttc ccagacgttg tggtgacttc
     9241 cctgtcgcga tatatgccat cagaccatgc tccagaaaac tcagcgtggc atcaaacatc
     9301 gctatatcga catgcgcccc tctctggctc ttttcgcggc cataaagtgc actcactatt
     9361 ccgctgaata aatagacacc gccgcataga tccgcaagag atgtaccaac gcgcactggc
     9421 ggagcatcag gatatcctgt ttccatcatt atcccgctca ttgcctgaat gatggtatcg
     9481 taggcaggag catcttttag cggaccggta tgtccgaaac ctgacgatga agcatatatg
     9541 aggcgcgggt tgatttcttg aagcgtttcc catgaaaacc ccagtttttc cattgtacct
     9601 gggcgaaaat tctcagctaa tacatcagct tgtttgagca tatttataaa tatactttta
     9661 tcgtaatcat tctttaaatc aagaaccaca ctctctttgc catgattaat aaaactgtaa
     9721 tagagtgact gtccatccac atagggacca aatgtgcggg tatcatcacc atgacccggc
     9781 ggctcaactt taattaccct tgcgcccata ttacaaagaa gttgagttcc gaaaggtcca
     9841 ttaaggacat gtgtcatatc gataactaat aagccttcaa acggcccttt gctttcatta
     9901 tttgtcattt gcttatgcct tgaaaaaagc cgaaggtgaa aagacacttc ggcatttatg
     9961 taaaatcaat aacatgatta attatgaaac cagtcgactg acataaatcc acaagggagc
    10021 cgtgacgaca aagcccagta cgctcacagc caatgacgct gtaccagtac gggtgtagac
    10081 attaaaccgg ctggcaataa taattccgga gaatgccggc ggtaatgcgc ctgccagtac
    10141 catcatctgc agatgttcgc tgttcaaatg acatgccata ccaacgagaa gcagtgccag
    10201 tggcatcaga atcagcttca ggaaggtgtt ataaacaatt tcggcactga actcgaattt
    10261 atgtgcagcc agagtcaacc ccgcagcgaa taccgctacc cctgagttag ccttcgcaat
    10321 cagattaaag gttgggtccc atgctgccgg aatttttacc ccaaccaaca ccaggatcgt
    10381 tgccagaaca ggtgcccata ctactggctc ctttgccgca gaaattaatg cactcagatt
    10441 actattcttc ttaccatccg ctcctgaaga aggattcagc aaatacagac caataggaat
    10501 agtaattgcg ttaacaataa tagaaataat tgctaccact aaacctgttg atacggaatc
    10561 accataaata ggatcgagaa ctgcaaaccc caggaatcca atggtaggtg aacctgcaat
    10621 taatgcacat acagctgctt ctgcatgggt acgtttaaaa aatttgtagc aaccgaacca
    10681 ggagaaaaag aaacatccga caataaccac aagtgataca agggtcagac gagtgtccgc
    10741 aaaaatcatt tccctgtttg cccgagtaat agatacaaat agagccgcag gaagcgcgta
    10801 gtttaatacc agtttattaa atgcgcgggc ttgatcttct gaaaaagttt ctcgtctgcc
    10861 gctaaaataa cccaacagca tgattacgat aataggcaat aaatcgccaa taaaaaatgt
    10921 tagcataata gtatttccga taagggtccc ctcaccatcg cataatgaat tggcaagagg
    10981 agacgatgtc agtatttaat taccagcgac ttgttttggg ttaagtttgg taatatggcc
    11041 actttcagtt cctgctgcag ggtcgatgac cacattaata atggtcggtt tgcgcgactg
    11101 aataccggtg gttaaagcat gacgaagttc atctgtcgtg gtgacgttat agccaacgcc
    11161 acgaaacgca tccattaatt tgtcatacct tgcatggtgc aacagatccg ttggagatgg
    11221 tgcaccagcg ccactgagat caacaccgtc tcctctgtag atgccgccat tattaaaaat
    11281 aacgatcgtc accggcaggt tatatcgaca aatcgtttca atttccatcc cactgaaacc
    11341 aaaagcacta tcaccttcaa tggcgacaac cggagaacca gaagtcacgc tagcaccgat
    11401 ggcatagccc ataccgatgc ccatgacacc ccaggtacca caatccagac gacgacgtgg
    11461 tttatacata tcaataatat ttcgtgcatt atccagggta tttgcacctt cattaactaa
    11521 ataaatatcc tggttctcgc gcaatacgtc gcgcacagaa cttaatgcat taaagtaatt
    11581 taatggttga gtatctgtac ttaatttttc atgcattttt tgtgcatttt gctgcttgtg
    11641 gatatttaaa atatcgcgcc ataccagtgg agtcgtaaat gtgttttgtt tcagttctgc
    11701 cagcatacct tgcatactgg atgcaatatc accaatgact ggcacagcaa tggggcggtt
    11761 gctgtcaatt tcctgcggtt caatatccag ttgaataaac tgtgtatctg ccgcccatcc
    11821 ttttttaccg tgtgccaata accaattcag tcgtgcacca acaagcatga caacgtcagc
    11881 atttgccagg gcaaacgaac gcgcagctgc cgcagaaagt ggatgcgtat cttcaaggat
    11941 ccctttcgcc atagacattg gcaggaatgg aatctgagca ctttcaataa attcacgaag
    12001 ctgttcatca gcttgtgaat acgccgcgcc tttgccaagg ataattaatg gccgttcagc
    12061 ttttgctaaa agcgaaattg cgctagtgac tgacttcggg catggcaata atgctggcga
    12121 cggattttca actttaacaa tcgtggttaa cgcttcgtct ttttccatcg tcgcggccag
    12181 gacatttgct ggcaaatcaa gataaactcc gccagggcga cccgatacag agacccggat
    12241 agcgcgtgcc aatgcaatgc caagatcctg cggctgatta acgcgaaatg ctgctttggc
    12301 atacggtttt gccgcattca tttggtccag ctcttcataa tcaccttgct gtaggtcgac
    12361 gatcgcgcgg tcgctggagc cgctaatcat gatcatcgga aaaccattta ccgttgcgtt
    12421 ggccaatgcc gtcaaaccat tgaggaatcc tggcgcagaa actgtcaggc agatccccgg
    12481 tttttgggta agaaaaccgc ttgccgcagc ggcatagcct gccgactgct catgacgaaa
    12541 accaatataa cgaatgcctt ccgcctgggc atggcgtgcc atatccgtca caggaatacc
    12601 tacaacacca taaatagtgt caatattatt ctgttttaat gcttcaacga tgatatgcat
    12661 accatctgtc atttgaagtt gatctgacat gattaataac ctcgaaattt ttataaattg
    12721 ggagccagaa gcccccgtta atatcagatg gcgtggtttt gcttcattgc agcaatttca
    12781 tcgtcgctat aacctagctc ctgcaataca gcagcggtat gttcacctaa tagcggcgca
    12841 gctttaatat ccggcgtaaa agcagagaat ttcattggac aaccaacggt cagatatttt
    12901 ccacgcaacg gttgttccac ttcaacaaca ctgccacttt ggcgcaaaga gggatcaagt
    12961 gaaatttctt tcatacttaa aaccggtgca caaggaatat caaactgagt caaataggcc
    13021 accgcttcat gtttatcaat agtgacagtg tatttttcga tttcagcaaa aatatcgaaa
    13081 atatgtggct gtcgggcatg ggctgtactg tatgccggat cggtaatcca ttctggtttg
    13141 ccgatggctt tacaggtgtt ttcccagttt tgctcctgaa tagtgaaata aatataggcg
    13201 ttcggatcgg tttcccagcc tttacatttc aggatccagc caggctgacc gccaccacct
    13261 gcattaccac cgcggggaac tgcatcacca aatgtaccat tcggatactg cgggtattct
    13321 tccagataac ccaatttatc gagacgctgc tggtcacgta atttcacgcg gcaaaggttc
    13381 aatacggcat cctgcattga catggtgact cgttgcccac gccccgtttt ttcgcgatgc
    13441 agcaaagcag caagcaaacc gatcagcaaa tgcattccgg tgttgctgtc acccaacgct
    13501 gcagcgctta ccagcggcgg accatcccaa aaacccgtag tggatgccgc gccacccgct
    13561 gcctgagcaa cgttttcata ggcttttaca ttcacataag gcgaacactc atcaaaccct
    13621 ttgatcgaac caaaaatcag acgtggattg atttcttgaa tatgctccca ggtgaagccc
    13681 atgtgatcaa tggcccctgg atgaaagttc tcgactaaga tatcagcttc gcggatcagc
    13741 ttttccatta cctctttgcc ttccgctgtt ttggtattta actcaataga acgtttgtta
    13801 ctgttaagca tggtgaagta aagcgcatcg atatcaggaa tatctcgcag ctggtgacgc
    13861 gttacgtcac caacgccggg acgttcaatt ttaataacgt cagcgccaaa ccaggccagc
    13921 atttgagtac aagatgggcc agattgcaca ccggtgaaat cgagaacttt aattccttga
    13981 agtggagttg acatagcaat acctattagt taattaaata tgtgttaatt tagcgtttat
    14041 ttaaggcgta atatcttcta taaataaccc gcaaaatatc gtcgtagctg cactcgatat
    14101 ttttaaaatc ctgtatcaag tgctgaaaat catttcatcg ccacttccaa ttcctttctg
    14161 catggtaagt agtgaggacg aaaagaagaa tatgataaat attgattcgt ggtaatcaga
    14221 gagagtcgcg acatttgtga tgttattctt acaactgtaa gaagcgccgg tgatttacaa
    14281 gtaagggtat gattacgctt tttaaagcgt accataaaac ataacataat aaatgatttg
    14341 tgatgacacg ggattattgg ggtttacttt gaggaagata aaatgcgtgt ttatagaacc
    14401 gcgagcgcca aataatttta tgaccacaac ctgtcgtatt gtggtcataa aaaattgtcg
    14461 gctggaggga gttaaatcct ttacctcaat atactgcctg tcagcattag ttcacaaaca
    14521 gtgcttcgct gtcgacgatg attccatctt ccgcaccttt cagcgccagg tttgcctcgt
    14581 agtattgctt tttatcaagc agtttctgcg catcagcaag tgcattacga gtctgtttaa
    14641 gcggcatcag atattggttt tccattacgc caacaccggc caggcgaagt tcttccattg
    14701 cgcccttttt atcccctttc gccatttttt cattggcaat ttttatcgcg gcttcttttt
    14761 cgggagttgc tacataactc tcagaaattc cgacagatgc attaatgaca atatattggt
    14821 catcattgag attggtcttt ttacctggct tagcgaactt tgcccattcg gtactgtcat
    14881 ctgacagcaa agcggaagct tcattggtca gttcttttgc cttttctgga tcgccatgaa
    14941 acagagccag gcgcgctacc tgcacgtcac gcatcgcata taaaccttgt tcagagatac
    15001 gctctgctgc tgccgttttc tgggtttgct gaacttgctg agtttgctga gtttgctgtt
    15061 gtgccgctac cggtgcatta tcagcagccc atacagttga agatgccaga attgctgtga
    15121 ccatcgtagc cataattaaa cgtttcatac ttacctcatt ctttaacagt tttagaaaac
    15181 gccgccatca tattgggtga gcatgaaatg agaaatcaga gagcaacggt cattttgaaa
    15241 gggttatctt acagttgtag ttattatcgc aagaagtatt ttagtcttac aattgtagga
    15301 atctgcttcc tgtcgcagag aactattctt atgtaccaca aagagatgtc atttattata
    15361 tcgatgtaaa tattgattaa ttatatgtaa atacagccat aacatcgtag gttctcagtg
    15421 aaccattttc aggataactt tctatgaaag taaacttaat acttttcagc ttatttttat
    15481 tggtctatat tatggcatgc aatgtttttg cattttccat ttctggtggt gtaagtgaga
    15541 ggagctataa agagactgaa aaaacatcag cgatgacgac cacacactct acaaaacttc
    15601 agccatcaca ggcgattttg tttaagatga gagaagatgc gccaccatta aacctcacag
    15661 aagaaatcac gcctacttat ccgacaaagg cgaattatct tattcatcct gtgcgatagc
    15721 gtctgtcaca tggcaggtga acaatcatgt ctgatgctga tgtgaacacc tgcccaaatg
    15781 agccatcctg acccactatc gcctgccata ccccaccata aagcgaatca tcaagccaca
    15841 agccggggca agaaacataa atatctccgt cactagttca ttaccgccag ttaaccacat
    15901 ggtgaagtaa taaatgagtg cagcgaggat caccatgccg aagaaagaag agaccgccga
    15961 taccacgttc agtacctgac cgatgtagcc gcttacgcag acaatacaca gggcgagaaa
    16021 catccataaa taaatcatag ctcaacctcc tgccgcgcta aaaaacctgt aacttcagtc
    16081 actttaatag taagacaatc cttaacctct ggcaattttt gctaagcatc aatgaaaaca
    16141 gcatgttaaa tgcaagactg ttgtgtacgg aaaaatattt actttgcacg attaataatc
    16201 agtagctgaa agcagtcagc gagtgataaa gtacagatac cgcattcatg ctcctcgtgg
    16261 ttatgtcctg actagtcttt acacacttta caggaaccat tgtcgtacat gatggcccaa
    16321 ccaattgagt gtaccgctgt cgacatggaa tcttctgcga tacaacaatt cgtatctaca
    16381 gaaggtaact atgtttccac aatgcaaatt ttcccgcgag tttctacatc ctcgctactg
    16441 gctcacatgg tttgggcttg gtgtactctg gctttgggta cagcttcctt atcctgttct
    16501 ctgctttctc ggcacgcgta ttggcgcaat ggcgcgacca ttcctgaaac gtcgtgaatc
    16561 tatcgcccgt aaaaacctgg aactttgttt cccgcagcat tctgcggaag aacgcgagaa
    16621 gatgattgcc gaaaactttc gttcactcgg catggcgctg gtagaaaccg gcatggcatg
    16681 gttctggccc gacagtcgcg tacgtaaatg gtttgatgtt gaagggttgg ataaccttaa
    16741 acgcgcacaa atgcaaaatc gcggcgtaat ggttgtcggc gtccatttta tgtcgctgga
    16801 actgggcggc cgcgtgatgg gactgtgcca accaatgatg gctacctatc gtccacataa
    16861 taatcagctg atggaatggg tgcagacccg tgggcgcatg cgctctaaca aagcgatgat
    16921 cggcagaaat aatctgcgcg gcattgtcgg tgcactgaag aaaggtgaag cggtatggtt
    16981 tgctcccgat caggattatg gtcgtaaagg cagctccttc gcgccgttct ttgcggtgga
    17041 aaatgtcgcc acaaccaatg gcacctatgt tctctcccgt ctctctggcg cagccatgtt
    17101 gaccgtaacg atggtaagaa aagcggatta cagcggatat cgtttgttca tcaccccaga
    17161 gatggaaggc tacccgacag atgaaaatca agccgctgcc tatatgaaca agattatcga
    17221 gaaagagatc atgcgcgcac cggagcagta cctctggatc cactgtcgct ttaaaacgcg
    17281 cccggtggga gaatcgtcgt tgtacattta acaaactgct cttactcttt ccctcctcaa
    17341 atcggccata ataacctcat ggtttttatg gcttttttct taaggttttt tccttcgctg
    17401 aatatctaat tattaccgtc agaaattaaa ctgtcgccga tatgagacac ttgtaagtta
    17461 ctaaaagtaa ataagaaaat gcctcttgtc tcgtttcagt tttaggcgta cattagcgcc
    17521 gtctcgagca gtttagctca gaattctggg gtgatgccca cggtaaaata cagggctttt
    17581 aaggtcgctt tgcccagtgt caactcactg tatcaggtgt aatgaagtca ttcaggcgta
    17641 acagcaatta cgcggagaga tgtaaagtga aatatttctt tatgggcatt tcttttatgg
    17701 tcatcgtttg ggccggtact tttgccctga tgatctaaaa gcaaactgtg caatgaaaaa
    17761 caggagccat caggctcctg tttgcatttc tgacgacggt gtttattccg cgttttcgtg
    17821 aatatgtttg ctgctggcgg gtagtaaacc gtcggcacgg aacatcgctt taatccctct
    17881 gatggcctga cgaatacggt cgcggttttc aatcagtgca aagcgaacat gcgtatcacc
    17941 atagtcgcca aagccaatcc ccggcgagac acagaccttc gcttcgttaa gcaatttctt
    18001 ggcaaatacc agcgagccca tggcagcata cggttccggt attttcgccc agacatacat
    18061 cgaagccttc ggcatttcaa ctatccagcc cgcttcatgc agccctttaa ccaatacatc
    18121 acggcggcgt ttgtactgtt cggcaatgtc gcgcacgcac tgttgatcgc cctccagcgc
    18181 cgcaatcgcc gccacctgca acggcgtaaa ggtgccgtaa tcgtgatagc ttttaatacg
    18241 agccagggcg ctgaccagcg ttttgttgcc aaccataaag ccgatacgcc agcccgccat
    18301 gttgtagctt ttcgacagcg taaagaactc gactgccaca tcgcgtgcac ccggtacctg
    18361 catgattgac ggcgctttcc agccatcgta gacgatatcg gcataggcca ggtcgtggac
    18421 caccagcaca tcgtagcgtt tcgccagcgc cacaaccttt tcgaagaact ccagctccac
    18481 gcattgcgcg gttggattcg acgggaagcc gaggatcatc atcttcggtt tcggataact
    18541 ttcacgaatg gcgcgctcca gttcgttgaa gaaatcgaca ccttccacca gcggcactga
    18601 gcgtacctgc gccccggcaa tcaccgcgcc ataaatatgg atcgggtaac ttggattcgg
    18661 caccagcacc gtgtcaccat gatccagcgt cgccagcatc agatgcgcca ggccctcttt
    18721 cgaaccaata gtgacgatgg cttctgattc cgggtcgatt tcaacgtcgt agcgatcctg
    18781 ataccagcgg gaaatggcgc gacgtaaccg cggaatgccg cgtgaagtgg agtaaccatg
    18841 cgtgtcctgg cgctgggcca cagtacataa tttttcgacg atatgcggcg gagtcgcacc
    18901 gtccgggtta cccatgctga aatcgataat atcttcgccg cgccgacgcg cagccatttt
    18961 cagttcagcg gtgatgttaa aaacgtaggg cgggagacga tcaatgcgcg taaagcgacg
    19021 ttcagggcga gtgtcagcca taatttcctc agattaacgt tagcgcccgg accgtccgag
    19081 cgacgctgcc acaatggtgg ctgttttgaa aatagcctga ttaatttctg tctgtccagc
    19141 gttttagaac atttttatca atggctttaa atgaggaact ccacttcaaa aacaggaaat
    19201 ttttctacgc taacaagaaa attaccacca attttattaa cattataatt tcaataaatt
    19261 taccagccag aagtgcatta tcaagagata cattgctaat acaccattta tctttcaatc
    19321 cccttttaaa tttatggttt ttcctcaaaa acggcctgct ggtcatcagc cttcaggtta
    19381 cctatcatag aggtttaatc cttattcaga gtcacccgtg cacgaaatat tcaacatgct
    19441 gctggcggtc ttcgatcggg cagcgttaat gcttatctgc ctgttctttc tcatccgtat
    19501 ccgcctgttt cgcgagctgt tgcacaagtc ggcacactcc ccaaaagagt tgcttgctgt
    19561 taccgccatt ttctcgctgt tcgccctgtt cagcacctgg tccggcgttc ccgtagaagg
    19621 ctcgctggtg aacgtacgta ttatcgcggt gatgtccggc gggattctgt ttggcccgtg
    19681 ggtaggcatc attaccggcg tgattgcggg tattcaccgg tatttaattg atatcggcgg
    19741 cgtgacggcg atcccctgct ttatcaccag cattctggcg ggttgtatat caggctggat
    19801 aaacctgaaa atccccaaag cacagcgctg gcgcgtcggt attctcggcg gcatgttgtg
    19861 tgagacgttg accatgattc tggtaattgt ctgggcacca actaccgcgt tggggatcga
    19921 tatcgtctct aaaatcggca ttccaatgat cctcggtagc gtctgtatcg gctttattgt
    19981 gcttctggtg caaagcgttg agggcgaaaa agaggccagc gccgcgcggc aggccaagct
    20041 ggcgctggat atcgccaaca aaacgctacc gctgtttcgc catgtcaata gcgagtcatt
    20101 acgcaaggtc tgcgaaatta tccgcgatga cattcacgcc gatgcggtgg cgattactaa
    20161 taccgatcat gtgctggcct atgttggcgt gggtgaacat aactatcaga atggcgatga
    20221 cttcattagc ccgactaccc gtcaggcgat gaattacgga aaaatcatca ttaaaaacaa
    20281 tgatgaagcc caccgcacac cagagattca ttccatgctg gtgatcccat tgtgggagaa
    20341 aggcgttgtg accggaacgc tgaaaattta ctactgccac gcgcatcaga taacctcgtc
    20401 attacaggaa atggcggttg gtctgtcgca aatcatctcc acgcaactgg aggtttcacg
    20461 cgccgagcag ctacgtgaaa tggcaaataa ggcagagctt cgcgccctgc aaagcaaaat
    20521 taatccccat tttctgttta acgctctgaa cgctatttca tcgtcaatcc gtctgaatcc
    20581 ggataccgct cgccagttga tctttaatct gtcgcgttat ctgcgctata acattgaatt
    20641 aaaagacgat gagcaaatcg atatcaaaaa agagctgtat caaattaaag actatattgc
    20701 cattgagcag gcccgctttg gtgacaagct gacggttatc tatgatattg atgaagaggt
    20761 gaattgctgc attcccagcc tgctgatcca gccgttggtg gagaacgcca ttgtccacgg
    20821 tattcagcct tgcaaaggta aaggcgttgt caccatcagc gttgcagagt gcggaaatcg
    20881 ggtacgcatt gcggtgcgag ataccgggca cggcatcgat ccaaaggtga ttgagcgggt
    20941 cgaagcgaat gaaatgccgg gcaataaaat tggcctgctg aatgtccatc atcgcgtgaa
    21001 gttattgtat ggcgaggggc tgcatatccg ccgcctggag ccggggacgg aaattgcgtt
    21061 ttacattcct aaccaacgca ccccagtcgc ctcacaggct acgttattgc tttgagccgg
    21121 agtgatattg tgaaagtcat cattgttgaa gacgaattcc tggcacaaca ggaactgagc
    21181 tggctaatta aagagcacag ccagatggag attgtcggca cctttgacga cggtctggac
    21241 gtgttgaagt ttttgcagca taaccgcgtc gacgccattt ttctggatat caatattccg
    21301 tcgctggatg gcgtgttgct ggcgcaaaac atcagccagt tcgcccataa accgtttatt
    21361 gtgttcatca ccgcgtggaa agaacatgcg gtagaagcgt ttgaactgga ggcgtttgac
    21421 tacattctca aaccgtatca ggagtcacgt attaccggga tgctgcaaaa actggaagcg
    21481 gcctggcaac aacagcagac cagcagtacg cctgccgcga cggtaacgcg tgagaatgac
    21541 accattaatc tggtgaaaga tgagcgaata atcgtcacgc caattaacga tatctattac
    21601 gccgaagcgc acgagaaaat gacctttgtc tatacgcggc gtgaatccta cgtaatgccg
    21661 atgaacatta ccgaattttg cagcaaactg ccgccgtcgc attttttccg ctgccatcgc
    21721 tcattttgtg tcaatctgaa caaaatacgc gaaatcgaac cgtggtttaa taacacctac
    21781 attctgcgac tgaaagatct ggattttgaa gtgccggtca gccgcagcaa agtgaaagaa
    21841 tttcgccagt taatgcatct ttaatcggga actttcatga aagcaccagg cttgcccgcc
    21901 gatcaacaat ttttcgccga tctgttcagc ggcctggtgc ttaactcgca actactgggg
    21961 cgcgtctggt ttgccagcca gcctgcctcg ttaccggtgg gcagtttatg tattgatttt
    22021 ccccgtctgg atatcgtgct gcgcggcgaa tacggcaacc tgctggaagc aaagcagcag
    22081 cgaatggtgg aaggagaaat gctgtttatt ccggcacgcg cggctaattt accgatcaac
    22141 aacaaaccgg tgatgctgtt aagcctggtg ttcgcaccga cctggcttgg gttatcgttt
    22201 tacgatagcc gcaccacgtc gttgttgcat cctgctcgcc agatccagct tcccagcctg
    22261 caacgcggtg aaggtgaagc gatgcttacc gccctcactc atcttagccg ttcgccgctg
    22321 gagcagaata tcattcagcc actggtgtta agtttgctgc atctttgccg caacgtggtg
    22381 aatatgccgc cgggtaattc gcaaccgcgc ggcgattttc tctatcacag catttgtaac
    22441 tgggttcagg ataattatac ccagccgctc acccgcgaga gcgtggcgca gttttttaat
    22501 atcacgccca atcatctgtc aaaactgttt gctcagcatg gaacgatgcg ttttatcgag
    22561 tatgtgcgtt gggtgcgaat ggcgaaggcg aggatgattt tgcagaaata tcatctgtca
    22621 attcatgaag tggcacagcg ttgcggtttt ccggatagcg actatttttg tcgcgttttc
    22681 cggcgtcagt ttggtctgac gccgggagag tacagcgccc gttttcaggg ctaacgtcag
    22741 aaggttaatt ctgtttccag cagcgtcagg atactttgtg catcttgcgc ggcaaacaac
    22801 gactggcgga agtttttatt caccagttta cgcgccagct gcgagaagac tttcacatga
    22861 ttcatccctt cgttagcacc cagcgtcagc atgatcacca gttcgacttc gcccatttct
    22921 gactgccagt caatcggttt cgccagccgg gcaatgctga tactggaatg acggatccac
    22981 tgagatttgg tgtgcgggat cgctacgcca aaaccaacgc cggtggtaac aatctcttcc
    23041 cgctgccaga catcttcttc cagctcaaac ggatgttcag tgcgcccgtt aacgccgagg
    23101 ttgccgcaca ggaactggat cgcctgctct ttattgctaa aatcctgatc aacaaagata
    23161 ttctccagcg ccagcagtgg gcgaacgtct tcttccggcg taaaggcggt gagtaacgct
    23221 tcaatttcct gcgcactgcg gcattcacat gcctgacgcg ccagttcccg acacgcctcg
    23281 ctatccagtt gacgaagctg gcttttcacc gccggaatac gcgggctact catactcagc
    23341 tcatccaggc ccagcccaag cagtagcggc agataacggc tttcaccgcc cagttcaccg
    23401 caaatgccta cccatttgcc ccgctgatgc gcagtggtaa ctatttgctg caacatgcgc
    23461 aggaacgatg gcgtaatcgg gttatatagc ggcgatacgc gcgggttatt acgatcgacc
    23521 gcatacagat actgggtcat atcgttggag ccgatactga agaaatcgac ctcatcgcag
    23581 aagtggtcga tgatgtagca caccgacggc acttccacca tgatcccaag cgtaatcgtc
    23641 tctgcatgac gcaggccatc gcgcttaagc tcaacgatcg ctttttggat ctcgcccttc
    23701 acccataaga tctgatcgag actgtggacc atcgggatca tcaactgggc gttgccaaaa
    23761 ctggcggcgc gcaaaatcgc ccgtagttga gtgcggaaca ggccagcaaa ttccgggtaa
    23821 atacgtaccg cgcgatagcc gaggaacggg ttctcttcct ggggaatatt cagataagga
    23881 atgcttttat cgccaccgat gtccattgtg cggaagataa tcggcttgtc gcccgctgcc
    23941 agtagcacct gctggtaggc ttcaaattgc tcctgctcgt ccggcgcgct gtcgcgatcc
    24001 atatagagca tttcagtacg gaacagtcca acgccttccg cgccgttggc aaacgcgcct
    24061 ggcgcttcca gagcggtgcc gatattcgcc gcaatatcaa tacgcttgtt atcacgggaa
    24121 taggccagct gcgcggcggc ctgcgcctgt tgtttttggc gtttatccgc cagcgtctgt
    24181 gcgacctgat aataaccgct caccgcgtca ttcgggttaa tcgccagcac gccgcactgg
    24241 gcgtcaagca ccgccggttg cccggcataa cgggcaatcg catccagcgg caggccgctc
    24301 agtaccggga tcgccgaggc gcgggccaga atcagggtat gcgaggtgcg cccagttttc
    24361 tccagaatca tgcctgcaag attttttaaa tcgaggctca aaaactggct tggggttaaa
    24421 tcctcggcta ccagaatagt cggtttttcc agcaccagat tgttgcgcgg cttcagttcc
    24481 ggccaggtga tatgcagcaa ctgttcgcta atatcgcgaa tgtcgctaac acgttcgcgc
    24541 agataatcac tggcagaagc agaaagtttg gcgcaaacct gctccatatt gctgatgatc
    24601 gccgccccca gcccttgatg ctgttctgtc atcagacgac ggatattgcc cgcgaattca
    24661 tcatcctgaa tcagcgacaa atgggcgcta aggatagttt tgctttcgcc gtcactctca
    24721 cgcaattgct ggttcagttg ctcggcaagc gttgcaaggc tgtgctccag ccgggtggaa
    24781 tcttgcgcac tggcggggat tgcccgataa ctgtcgaggc tgtcgctttg taacagggtc
    24841 agcgtaccca cgccgacgct gcttgccagc acattgccgt acagtaaatc cgggttcagg
    24901 cggcttaatg aacgcggcag cggatgcgcc gtcagttctg cctgcgttgg ctggatgctg
    24961 tcgctgtcga taaagcgcac ctggatgtac tcttccaaca cgcgctgcgc ctgctcttca
    25021 tcgctgccgc taatgttcag gctgcaactg tcattaaata aagtaccggt gccaatcagc
    25081 gccagcgagc ttttcgcatc ggcctttgcg ttctggcgat ggttgataaa ggtgatttcg
    25141 ctttgccact ggctgcactg ttctttaagt tcccacgccg gacgagcatg aagaccgttt
    25201 ggcaaaggac agagaaattg aatcgttaac ataaccgctc cgggttaatc aggagatcat
    25261 ctgaaatccg tcagttgaac aaccgtctca cgcgtaagac gtttaataag ggcagataaa
    25321 agctgctgca tctgcaaaat gtcacggcaa tcggcaatcg atgcggcgca atgtccgtgg
    25381 cgggttgctg gccccatcac cactgtaggc acgccggtgc cggttaagtg caccgctccg
    25441 ccgtccgtgc cgccgttgct gaacatatct gcctgtaacg gcacgccaat ttctgccgcc
    25501 acggtttcga tccacgcggt aagttttggc ggcgcaatca gcgacttgtc gcttaacacc
    25561 agcatcggcc cgttacaaat ctgacgatgg ttagccgcgc cataatcaaa gtttttcgcc
    25621 cagcaggcgg tatcaagcac aatggcgaca tccggcgaca ccgcgcgggt ggcagtttgc
    25681 ccgccgcgta cccccacctc ttcgctggaa ctggccacca gccacacttc cgcaggcagt
    25741 tcagcgctgt gtagttcgcg cagtaacgtc accagcaggt agcaaccgag gcggtcatca
    25801 aaggctttcc ccatcacccg ctggtgaggg agaacctgaa aagttgtatc aaaggtgacg
    25861 cgatcgcctg gacgaattcc cgcctgcatc acttcgtcat acgagcgcgt accaatgtcc
    25921 acgcgcatgg cgctgacgtc attcccctgc cggtcgccgt caagcaggcc tggaattttg
    25981 cactcttcac gggtggtgat gcgcaccggc tgcagctggc gggcagccat gcgcacgttg
    26041 ccaaccggca gcacatcaat tgccccttcg cgggagatgc tgcgcaccat aaatcccact
    26101 tcgtccatat gggcgcagat catcaccttc ggccctgtcg attcattgag gcggatcagc
    26161 accgatccca gaccatcaaa tcgcacttct ttttgcaggc gatccgcttc ttccagcagg
    26221 atctgccgca cttcctgttc cgaggaggcg atcgcatctg cctcgctcaa cgcttttaat
    26281 agcgataaat ccattatgcc tctcccgtga gcaacactgt tttcggcatg gcgtagagca
    26341 cttctgcgcc ttgcggggta accagcacaa catcttcgat gcgcacgccc ccttgccctg
    26401 gcaaataaat ccccggctcc acggtcagca acatgcctgg ctgtagcgtc gtggtgtccc
    26461 gcggtgaaaa acgcggatct tcatggactt caatgccgat agcgtgacca gtgttatgac
    26521 cgaaatagtc gccaaatcca gcctcggtaa tgacccggcg cgcggcgtcg tcaatctgct
    26581 ggcagcgcac gccggggcga attgcagaga ttgccgcgag ctgtgcctgc aggacaatct
    26641 gatagacgtc aaacagcggg tgagattcgg cgctcacccc ttcgccattc accagcaagg
    26701 tgcgcgtcat atcagagcag tagccctgat acagcgcacc aaaatcgaga gtgacaaact
    26761 cgcccgctgc aacaatcttg tcgctggctt tgccgtgcgg cagcaccccg cgccagccgc
    26821 tggcgacaat ggtgtcaaaa gaggcttttt ctgcgccctg ctggcgcata aaccactcca
    26881 gttcagcggc tatctcgcgc tcgctcatcc ccgcctgaat aaagcggcga atatgctccg
    26941 ctccgcgatc ggcaatccca caggcgaggc ggattatctc cacctcctct ggcgttttga
    27001 tttgccgcag cacatccggc gtggcgctaa ccagtttcgc attgagttca gacttccagc
    27061 ggtgcgcggt ttcccaactc acctgctggc cttcaaaacc gagcgtttgc aactgctcat
    27121 cggcaatgat ttgattgacg atagtggtaa gcgtgttcgt cgcgtcaagc aaatgcagct
    27181 ggtagccttg cgcacgggct tccacctcaa cgaaatagct cgaatctacc agaatgtgcg
    27241 cactttcacg gctaataacc acatagcctg atccggtgga gatccccaga tgcggctgtt
    27301 tgttctgccg tgaggagaga agcactgcat ccagttgttg ctccttaagc cagtcgcgca
    27361 gcgaagcgag taatgtcatc tggattttcc ttcttacagg ctatcgatta acaatttgcc
    27421 tttacggaac atcatcagac gcaggaacac caccatcagc gcagttatga ctgcccccag
    27481 cgcgatcccc gccatataga cgccaaggtt agtgaccagc ggccatgccc agatagcgga
    27541 ttccgggaac cattgcactg cgcccagcca gacagcggcg gttgagccga caatcgcgcc
    27601 gaccatatac gacggaatgg cggtgatggg gctttccagc gcaaacggaa tcgcaccctc
    27661 actaatcccc ataaaggcaa ggaacatcgc ggtcttaccc tgcggataaa gttgagcgtt
    27721 gaacaggcgt ttgccggtta aacgacggtc aataatggtc gccagcccca gaccaatcgg
    27781 aggaataacg atagcgatag aacgagcggt gaccggtaat acgtggtcag tggtaaagct
    27841 gaaggcaacg aaacctgcgg ctttgttgat cgggccacca aggtcgattg ctgtcgccgc
    27901 ggcgatcccc attgcgtaca tcagcgcgcc cttctcacct gcggcagtca gcacggtacg
    27961 gataacgccg ttgatccagc cgccaaaggg ggtaatgacg tagtacatcg ccagcatgac
    28021 aaaaatggcg gaaagaatcg gtagcaaaaa tgtggttttg aacgctaaca ggaaatccgg
    28081 taactggatt ttctggttca tccacttcac aaggtaaccc gcgacgatag aaataatcag
    28141 cgcaccaatg aaggtggacg gcaccggcga agaggtcgcc cattgcatag tgctgggatc
    28201 gaagttcagc agttgcgtcg gctgggtaga catcaagccg ccgataaaac cagccgggaa
    28261 cgccagtttg ccaccgatag agttcgccac aaaagcagca aacatcggaa tggcaaaacc
    28321 aaacaacacg ccgccgaagg actgcgacag ccaggcaaat ttcagtaagg agaggtcgaa
    28381 accggagaat ttcccgctat taagagcatc catgatgccg atctcagcag gaattttaag
    28441 ccagctataa gcaatcagct ggctgaaagc gaggatcaca ccgcccataa ttaaggtcgg
    28501 caccatgcgt gaaatccctg acatcacatg ctgcgggagt tcaccccaga agctgctttt
    28561 agaggcctgc ggattcttaa ctgccgctgc cgcaccggat gcgccaggca caacggttgc
    28621 actgcgtttt ttaatggcca taacaattat ccctgtcgaa tattactgtt gttcagaggc
    28681 aatcatctct tcgatttctt tgatgatgcc cgcagcgttt ttaattgcgt cctgcaaagt
    28741 gatttcataa acgtcgcgtg attcgaaacg ctcattatct tccggggtaa ctgccacgga
    28801 gtggatgatg atggttgctt cggcgatatc ctgcgccgtc aggcgattct ggataccgtc
    28861 cgcgccctgg gtttcaattt tcacttcata accggcttct accgccgctt cttccaacgc
    28921 ctgagcggcc ataaaggtgt gagccaggcc cattgggcag gcacataagg caatcagttt
    28981 cttactcatc gttaattcct cattcagtga tagttgacaa gtgaaattat gaacgtgcaa
    29041 aacaaatcgc cgttaccaga caaataatgc atttactgga gaattaatcc cgctgtacgg
    29101 aagtgtgacg caggtcggcc ttgtgagggg acacaaagaa aaattgaatt aactgtatga
    29161 tttaaacgat tatcgggaga gttacctccc gatataacag gaaggattta cagaatgtgc
    29221 cctaaggtct ggcgtaaatg tgcaccggaa ccgagaagac ccggattgtc atggacgatg
    29281 agatacaccg gaatatcatg gacatattct ttaaagcgcc ctttatcttc aaatgcagca
    29341 cggaaaccag aggctttgaa gaactcaagg aagcgcggca cgataccgcc cgcaataaac
    29401 acgccgccaa atgtcccgag attgagcgcc agattgccgc caaaacggcc cataatgacg
    29461 caaaacagcg acaacgcgcg gcggcaatcg gtacagctgt cagccagcgc gcgttcggta
    29521 atatcttttg gcttgagatt ttctggcagg cggttgtcag ctttcacaat tgcgcgatac
    29581 aaattcacca gcccagggcc agaaagcacg cgctccgccg aaacatgacc aatttccgca
    29641 cgcaatattt cgaggataat ggcctcttct tcactattcg gcgcaaaatc aacgtgaccg
    29701 ccttcgcccg gcaagcttac ccaacgctta tcgacatgga ccagatgcgc aaccccaagc
    29761 cctgttccgg caccgtaaac cgcaataggt ttaccttcta ccggttctgc gccaccaaac
    29821 tgaatcagat gctctttttt cagcatcggg atcgccatcg atacagcggt aaaatcgtta
    29881 ataatttcca gatggctaaa accgagattc tttttcattt cggcaattga gaacgcccag
    29941 gtatggttgg tcattgccac ccagtcaccg gtaattgggc aagcgatggc aatacagccg
    30001 tctttcacct cgaccttatg ttcttcaaga taaacgcgaa tgaccgcttc gaggctgggg
    30061 taatcaagcc ctgaataggt cttagcctgc gagatttcac cactggcaat atcacacaga
    30121 gcaagacgtg cgttggtgcc gcccacatca ccgactaatg catactttat cattcttcaa
    30181 ctgctccgct aaagtcaaaa taattctttc tcacactgta aatacctggg ggcataacaa
    30241 caacgccgga aaggcaggct ccctgtaaat atcgatctgg gtcacacatt tactttatcg
    30301 tttcagcacc aattgcagcg atgccttttt gcaagctggg caaactaagt atctgacccc
    30361 gcataaggaa tagaacatgc tccatccgcg agccagaacc atgttgttat tatcgctccc
    30421 cgccgtggca attgggattg cgtccagtct tattctgatt gtggtgatga aaatcgcctc
    30481 ggtattacag aatttgctct ggcaacgact gccgggaact ctggggatag cccaggattc
    30541 acctctctgg atcatcggtg tattaacgct aacgggtatt gcggtggggt tggttatccg
    30601 tttcagccag ggtcatgccg gaccagaccc cgcctgtgaa ccgctgatcg gcgcaccagt
    30661 tccgccctct gcgctaccag gacttatcgt agcattaatt ctcggtcttg ctggcggcgt
    30721 cagcctgggg ccggaacatc cgatcatgac catcaatatc gcccttgccg tcgccattgg
    30781 cgctcgtctg ttaccgcgcg tcaaccgaat ggagtggact attttagcct ctgccggaac
    30841 catcggcgcg ctgtttggca cacctgttgc agcggcgttg atattttcgc aaaccttaaa
    30901 tggcagtagt gaagttccgc tatgggatcg tctctttgcg ccgttaatgg cggcagcagc
    30961 tggtgcacta actaccggat tatttttcca tccccatttt tcactgccca ttgcgcatta
    31021 cggacagatg gaaatgaccg atattctcag cggtgcaatt gtcgcggcga ttgccatcgc
    31081 agcagggatg gtcgccgtat ggtgcttacc acggttgcac gcgatgatga atcaaatgaa
    31141 aaatccggtg ctcgtgctgg gtattggcgg atttattctc ggtattctgg gggttattgg
    31201 tggaccagtt tcgctgttta aagggctgga tgagatgcag cagatggtgg caaatcaggc
    31261 tttcagcacc agcgattact ttttgctggc ggtaattaaa cttgccgccc tggtcgttgc
    31321 tgccgccagt ggctttcgcg gtgggcgaat cttcccggca gtgtttgtcg gcgtggcatt
    31381 agggttgatg ctgcatgagc acgtacccgc cgtaccagcg gcaataaccg tttcttgcgc
    31441 tattctcggc atcgtgctgg tggtaacacg cgatggctgg ttaagtcttt ttatggcggc
    31501 ggtcgttgta cccaatacca cattgctacc gctgctctgt atcgtcatgc ttccggcatg
    31561 gctgttatta gcaggtaagc cgatgatgat ggtcaatcgt ccgaagcaac agccacccca
    31621 cgataacgtt tagcaaaaaa tgctctcctt tatgatttaa gagttatggc gatgatacgg
    31681 ggctttacct ccccgtaata ttgcgttaac aggccgctga cggcttcgat cgtgaaggag
    31741 aataacaatg ttcaggtcac tgtttctggc ggccgccctg atggcattta ccccgcttgc
    31801 agcaaacgca ggtgaaatca ccctactgcc atcaatcaaa ttacaaattg gcgatcgcga
    31861 tcattacggt aattactggg acggtggtca ctggcgcgac cgtgactact ggcatcgcaa
    31921 ttatgagtgg cgcaaaaacc gttggtggcg tcatgataat ggctatcatc gtggctggga
    31981 taagcgtaaa gcgtatgagc gtggctatcg tgaaggctgg cgcgatcgtg acgatcatcg
    32041 cggaaaaggc cgcggacatg gacaccgcca ttaaacacat tcgccaatgg agcacaatgc
    32101 ctgatgcgac gctagcgcgt cttatcaggc ctacaaaccg gatctttaac gcgtagggcg
    32161 gataaggcgt ttacgccgca tccgccaata gtgccagatg cgacgctcat tcaactacaa
    32221 tcccagcgcc gtccccacca acaaccagat attcagcgcc acgaccagca ctacaatcac
    32281 ccagcctgtc tgttttacgc gtttgctgtt caccagatcg cccatcaact tgctgtcact
    32341 ggtgaaaatc agcagtggaa ccagcgccag ggcgatacca aaacttaaca gcacctgact
    32401 cataaccaga atccgtgtcg gatctaatcc catcagaatg acaataaatg acggcagcat
    32461 ggtgactgta cgacgcaccc acagcgggat atggaagcga atgaacccct gcatcaccac
    32521 ctgccctgcc agtgtcccca ctaccgttga ggacagtccg gcagcaacca gacttaaccc
    32581 aaagaccgtt gccgcagcat ggcttaacag cggttgcagc gtcagataag cctcatcaag
    32641 atcggcaaca ccagtatgac cagaaaagtg gaacgccgcc gcagctgtag ccatcatcgc
    32701 cagattgaca aaaccggcaa tagtcatggc gatagccaca tcccatttgg tggcggaata
    32761 acgttgttga cgcgaaccgc catgtaaatg ctgagtgagc gaggagtgca aataaatcac
    32821 atgcggcata atcgtcgccc ctaacacgcc tgctgccagg aagaccgctt ccgaagtagg
    32881 taaactcggg atcaccattc ctttacccag ctgcgccagg ttaggctggg agaaaatcaa
    32941 ctcgacaatg taagccgcgg caacaaacaa cagtaacccg ccaatcactt tctccagcgg
    33001 tttttgcccg cgacgttgca gcattaaaat caggaaagtc gcgatccccg tcagcaccgc
    33061 gccctgcaac aacgaaacac caagaatgag tttaaaaccg atcgccgcac caataaattc
    33121 cgccaggtcg gttgccatcg caataatttc tgcctgaacc caatagaacc acactacggg
    33181 acgcggatag tgatcgcgaa tctgctccgc cagattttta ccggtggcaa tccctagttt
    33241 ggcagagagg atctgaatca gcatcgccat caggttggcc caaacgacaa cccacagtag
    33301 ctgatagccg aagctggcac ccgcctgaat attggtcgca aagttaccgg gatcgatata
    33361 accaatcgcc gcaatgaacg caggtcccat taatgcgagc ctcatcttgc gcgccgcccg
    33421 tccgctgcta ctctcaacgc gatagttcgt catcttgtgc ctctaaaaca tagcctttgc
    33481 tatgtttcat gctatgataa acgagaatga ttatcaaatt catttaaatg gattgtggtg
    33541 atttctgtga tagaccagga ttatgactac gaaaagattg atgacttgaa tgttatggtt
    33601 atgtttaatg ttagcacatt tacataactt tcagcttcca tacacaacat agcagaaatg
    33661 tatgacagat cactattttt gaagcctgtc acaggacgtc attatagtgt gtgtcagatc
    33721 tcgttttcct taaccatgtt acatagaatg tgcacggaaa tttaacctgc ctcatatttg
    33781 gagcaaatat ggaccgcgtc cttcattttg tactggcact tgccgttgtt gcgattctcg
    33841 cactgctggt aagcagcgac cgcaaaaaaa ttcgtatccg ttatgttatt caactgcttg
    33901 ttatcgaagt gttactggcg tggttcttcc tgaactccga cgttggttta ggcttcgtga
    33961 aaggcttctc cgaaatgttc gaaaaactgc tcggatttgc caacgaaggg actaacttcg
    34021 tctttggtag catgaatgat caaggcctgg cattcttctt cctgaaagtg ctgtgcccaa
    34081 tcgtctttat ctctgcactg atcggtattc tccagcacat tcgcgtgttg ccggtgatca
    34141 tccgcgcaat tggtttcctg ctctccaaag tcaacggcat gggcaaactg gaatccttta
    34201 acgccgtcag ctccctgatt ctgggtcagt ctgaaaactt tattgcctat aaagatatcc
    34261 tcggcaaaat ctcccgtaat cgtatgtaca ccatggctgc cacggcaatg tccaccgtgt
    34321 cgatgtccat cgttggtgca tacatgacca tgctggaacc gaaatacgtc gttgctgcgc
    34381 tggtattgaa catgttcagc acctttatcg tgctgtcgct gatcaaccct taccgtgttg
    34441 atgccagtga agaaaacatt cagatgtcca acctgcacga aggtcagagc ttcttcgaaa
    34501 tgctgggtga atacattctg gcaggtttca aagttgccat tatcgttgcc gcgatgctga
    34561 tcggctttat cgccctgatc gctgcgctga acgcactgtt tgccaccgtg actggctggt
    34621 ttggctacag catctccttc cagggcattc tgggctacat cttctatccg attgcatggg
    34681 tgatgggtgt tccttccagt gaagcactgc aagtgggcag tatcatggcg accaaactgg
    34741 tttccaacga gttcgttgcg atgatggatc tgcagaaaat tgcttccacg ctctctccgc
    34801 gtgctgaagg catcatctct gtgttcctgg tttccttcgc taacttctct tcaatcggga
    34861 ttatcgcagg tgcagttaaa ggcctgaatg aagagcaagg taacgtggtt tctcgcttcg
    34921 gtctgaaact ggtttacggc tctaccctgg tgagtgtgct gtctgcgtca atcgcagcac
    34981 tggtgctgta agaccataca taaaaaagcc ggggataatt cccctaatgc cagtcagtta
    35041 agcaactgac tggctctttt tcggggctgt ggggtatttc cagggcctct cctttaccac
    35101 tctcggtaag gcccttcccc ttctcgtcgg ta
//
LOCUS       DAZPEB010000018        35088 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZPEB010000018 DAZPEB010000000
VERSION     DAZPEB010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35088
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.018"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..4112)
                     /locus_tag="ACHURJ_000810"
     CDS             complement(<1..4112)
                     /locus_tag="ACHURJ_000810"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005006078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFR1279869.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARS
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQLPLKGIADERRRRGETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGL
                     SATVRSASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASSTGEDSHA
                     GREGSIWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVD
                     AAHFESTSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLEL
                     GIDMGAVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVEC
                     MFAGRLENLTPPHNPLDVLAQQTVAAAAMEALQVDEWYSRVRRAAPWKDLPRRVFDAT
                     LDMLSGRYPSGDFSAFRPKLVWNRETGILTVRPGAQLLAVTSGGTIPDRGMYSVLLPE
                     GEEKAGSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPF
                     WRGEGNGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTNANIQGLIDEQRNATG
                     IVPGSRHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASD
                     DGIVARIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARALLMPGR
                     TPGHRTPLWQQRLRASQLLEIAQGYPDFPVILEILRECLQDVYDLPALERLMRRLNGG
                     EIQISDVTTTTPSPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVD
                     PGELLDPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEV
                     ASYLENLLAVKRIFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLR
                     DLFLRYLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRR
                     LRLRSLQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMR
                     VIEQLAGVGLPASLWESQILPARVRDYSSEMLDELLATGAVIWSGQKKLGEDDGLVAL
                     HLQAYAAESFTPAEADQANRSALQQAIVAVLADGGAWFAQQISQRIRDKIGESVDLSA
                     LQEALWALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHRARRGRPVYAQPISPLVS
                     YNTPNLAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTL
                     CRSMEDSGRIMRGRFVEGLGGAQFAERLTIDRWRDLATQATQTRHYEPPRV"
     gene            complement(4205..4852)
                     /gene="rnt"
                     /locus_tag="ACHURJ_000811"
     CDS             complement(4205..4852)
                     /gene="rnt"
                     /locus_tag="ACHURJ_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFR1279870.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMIAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(4955..5362)
                     /gene="gloA"
                     /locus_tag="ACHURJ_000812"
     CDS             complement(4955..5362)
                     /gene="gloA"
                     /locus_tag="ACHURJ_000812"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFR1279871.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFMEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(5443..6540)
                     /gene="nemA"
                     /locus_tag="ACHURJ_000813"
                     /pseudo
     CDS             complement(5443..6540)
                     /gene="nemA"
                     /locus_tag="ACHURJ_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
     gene            complement(6577..7176)
                     /gene="nemR"
                     /locus_tag="ACHURJ_000814"
     CDS             complement(6577..7176)
                     /gene="nemR"
                     /locus_tag="ACHURJ_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFR1279872.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYTAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            7279..7518
                     /locus_tag="ACHURJ_000815"
     CDS             7279..7518
                     /locus_tag="ACHURJ_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFR1279873.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            7567..8463
                     /locus_tag="ACHURJ_000816"
     CDS             7567..8463
                     /locus_tag="ACHURJ_000816"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFR1279874.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            8544..9065
                     /gene="sodC"
                     /locus_tag="ACHURJ_000817"
     CDS             8544..9065
                     /gene="sodC"
                     /locus_tag="ACHURJ_000817"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFR1279875.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVITPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(9066..11077)
                     /locus_tag="ACHURJ_000818"
                     /pseudo
     CDS             complement(9066..11077)
                     /locus_tag="ACHURJ_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(11077..11934)
                     /locus_tag="ACHURJ_000819"
     CDS             complement(11077..11934)
                     /locus_tag="ACHURJ_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFR1279876.1"
                     /translation="MSIKTIKYFSIIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEK
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LRGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(11937..12173)
                     /locus_tag="ACHURJ_000820"
                     /pseudo
     CDS             complement(11937..12173)
                     /locus_tag="ACHURJ_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
     gene            12374..12808
                     /gene="slyA"
                     /locus_tag="ACHURJ_000821"
     CDS             12374..12808
                     /gene="slyA"
                     /locus_tag="ACHURJ_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFR1279877.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(12855..13322)
                     /gene="slyB"
                     /locus_tag="ACHURJ_000822"
     CDS             complement(12855..13322)
                     /gene="slyB"
                     /locus_tag="ACHURJ_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFR1279878.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            13596..14705
                     /gene="anmK"
                     /locus_tag="ACHURJ_000823"
     CDS             13596..14705
                     /gene="anmK"
                     /locus_tag="ACHURJ_000823"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFR1279879.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            14803..15132
                     /gene="mliC"
                     /locus_tag="ACHURJ_000824"
     CDS             14803..15132
                     /gene="mliC"
                     /locus_tag="ACHURJ_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFR1279880.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            15191..15847
                     /gene="pdxH"
                     /locus_tag="ACHURJ_000825"
     CDS             15191..15847
                     /gene="pdxH"
                     /locus_tag="ACHURJ_000825"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFR1279881.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            15976..17250
                     /gene="tyrS"
                     /locus_tag="ACHURJ_000826"
     CDS             15976..17250
                     /gene="tyrS"
                     /locus_tag="ACHURJ_000826"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFR1279882.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            17309..18172
                     /gene="pdxY"
                     /locus_tag="ACHURJ_000827"
     CDS             17309..18172
                     /gene="pdxY"
                     /locus_tag="ACHURJ_000827"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFR1279883.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(18216..18821)
                     /gene="gstA"
                     /locus_tag="ACHURJ_000828"
     CDS             complement(18216..18821)
                     /gene="gstA"
                     /locus_tag="ACHURJ_000828"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFR1279884.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNETLKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(18927..20429)
                     /gene="dtpA"
                     /locus_tag="ACHURJ_000829"
     CDS             complement(18927..20429)
                     /gene="dtpA"
                     /locus_tag="ACHURJ_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFR1279885.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(21041..21676)
                     /gene="nth"
                     /locus_tag="ACHURJ_000830"
     CDS             complement(21041..21676)
                     /gene="nth"
                     /locus_tag="ACHURJ_000830"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFR1279886.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(21676..22371)
                     /gene="rsxE"
                     /locus_tag="ACHURJ_000831"
     CDS             complement(21676..22371)
                     /gene="rsxE"
                     /locus_tag="ACHURJ_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFR1279887.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(22375..22995)
                     /gene="rsxG"
                     /locus_tag="ACHURJ_000832"
     CDS             complement(22375..22995)
                     /gene="rsxG"
                     /locus_tag="ACHURJ_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFR1279888.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWEV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(22999..24057)
                     /gene="rsxD"
                     /locus_tag="ACHURJ_000833"
     CDS             complement(22999..24057)
                     /gene="rsxD"
                     /locus_tag="ACHURJ_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFR1279889.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSTLLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGADMNTLHLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(24058..26280)
                     /gene="rsxC"
                     /locus_tag="ACHURJ_000834"
     CDS             complement(24058..26280)
                     /gene="rsxC"
                     /locus_tag="ACHURJ_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFR1279890.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDARFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAIE
                     AAIARAKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAVPEEQVDP
                     RKAAVEAAIARAKARKLEQQQTNAEPEEQVDPRKAAVAAAIARAQAKKAAQQKVVNED
                     "
     gene            complement(26273..26851)
                     /gene="rsxB"
                     /locus_tag="ACHURJ_000835"
     CDS             complement(26273..26851)
                     /gene="rsxB"
                     /locus_tag="ACHURJ_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFR1279891.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(26851..27432)
                     /gene="rsxA"
                     /locus_tag="ACHURJ_000836"
     CDS             complement(26851..27432)
                     /gene="rsxA"
                     /locus_tag="ACHURJ_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFR1279892.1"
                     /translation="MTDYLLLFVGTILVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFTAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(27509..27949)
                     /locus_tag="ACHURJ_000837"
     CDS             complement(27509..27949)
                     /locus_tag="ACHURJ_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFR1279893.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVSYFKRSSRVKATFVNP"
     gene            complement(28035..28250)
                     /gene="ydgT"
                     /locus_tag="ACHURJ_000838"
     CDS             complement(28035..28250)
                     /gene="ydgT"
                     /locus_tag="ACHURJ_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFR1279894.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(28523..28648)
                     /gene="blr"
                     /locus_tag="ACHURJ_000839"
     CDS             complement(28523..28648)
                     /gene="blr"
                     /locus_tag="ACHURJ_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFR1279895.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            28861..29901
                     /locus_tag="ACHURJ_000840"
     CDS             28861..29901
                     /locus_tag="ACHURJ_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFR1279896.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(29936..30937)
                     /gene="add"
                     /locus_tag="ACHURJ_000841"
     CDS             complement(29936..30937)
                     /gene="add"
                     /locus_tag="ACHURJ_000841"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFR1279897.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNLARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRAGIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(31040..32212)
                     /gene="malY"
                     /locus_tag="ACHURJ_000842"
     CDS             complement(31040..32212)
                     /gene="malY"
                     /locus_tag="ACHURJ_000842"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFR1279898.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDSQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCNMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRIYLKDNLTYIADKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(32222..33814)
                     /gene="malX"
                     /locus_tag="ACHURJ_000843"
     CDS             complement(32222..33814)
                     /gene="malX"
                     /locus_tag="ACHURJ_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFR1279899.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQTQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPAAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYAIFRFAITRFNLKTPGRDSEVASSIEKAVAAAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            33989..35017
                     /gene="malI"
                     /locus_tag="ACHURJ_000844"
     CDS             33989..35017
                     /gene="malI"
                     /locus_tag="ACHURJ_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFR1279900.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
BASE COUNT         8788 a         9014 c         9131 g         8155 t
ORIGIN      
        1 acccggggcg gttcatagtg gcgcgtttgc gtggcttgtg tcgccagatc gcgccatcgg
       61 tcaatagtca gacgttcagc gaattgcgcg ccacccagac cttctacaaa acgacctcgc
      121 ataattcgcc cggagtcttc catacttcga caaagcgttt gcatcgatgg aaacccgcca
      181 gggatatttt cggctatcac cgcctgacga ctgatgatgc cgtagcggtc gagcatattt
      241 tccgccagcg ccagcatcct ttcggtatcg tttagtggct ccacctgcaa taacgaccag
      301 cgtccagcca gatttggtgt gttgtaagat accagcggcg agatgggttg cgcatagaca
      361 ggacgtccac gacgagcccg gtgactgcgg cgagttgagg tgcgtgcgtt ggaactgctg
      421 cgggtgaggg cgcgtaacgg tgcccaaatg tcgctggtga tgacgccttg ccagaccagc
      481 gcccataacg cctcttgcag ggcagagaga tcaaccgatt cgccgatttt gtcgcgtatc
      541 cgttggctga tttgttgtgc aaaccaggct cctccgtcag ccagaacagc gactatcgct
      601 tgttgcagcg ccgaacgatt cgcctgatcc gcttcggcgg gagtgaacga ttctgcagca
      661 tatgcctgta gatgcagtgc caccaggccg tcatcttcac ccagtttttt ttgccccgac
      721 cagataaccg caccggttgc cagtaattca tcgagcattt ctgacgaata gtcgcgtacg
      781 cgggcaggca ggatctggct ttcccagagt gaggcgggta aaccgactcc ggcaagctgt
      841 tcgatcaccc gcatcacgcc atctacgccc tcataaacgc ctggcgatgt tgaggcaaag
      901 agcgccgggc taccatcggt ggcgggtaat acgccctgac gttccagcag caatcgcgca
      961 taggtcgtgg ctgcaacggg acgcgtcgct tctctggcgg cttgcagcga gcgcaaacgc
     1021 agacgacgaa atacttcatc gctcacccag atgtcttgtt gcagattcat caccagaccc
     1081 tgttcacgca gttgctgaag ctgctcttcg acaatggcaa taccgagact aaactcatga
     1141 gccagttgtt cagccgtgac cagagcatga gcccggagat agcgcagaaa gaggtcgcga
     1201 agcgggtaac tgactctatg taaataaatc tctggcaatg actctggtag tcgtacgccg
     1261 agggcatcac gcagcctggc ggcatcatcc atacaggcaa gacgctcctg tccgctaatc
     1321 atcgctggga agattcgttt tactgcaaga agattttcca gatacgacgc aacctcttca
     1381 ctgctgcctg tatgccgttg cgccaggtct tcaacggtca ttggccccag ttcgcgcagc
     1441 aggtcgaaca atccttcttc acctttcgct cttctgccag gagccagtcg ttgcaactct
     1501 tcttccacct ggcgaatgac ctgcgggtcg agtaattccc ccggatcgac ctgtccgagt
     1561 agattgcgca gtaactcgct gtccagcgac agtacggatg cccggcgctc tgccagcggg
     1621 gcgtcgctct ggtacataaa ttccgcgaca tagccgaaca ataaacttgt ggcgaaaggc
     1681 gagggcgtag tggtcgttac atcggatatt tgaatttcgc caccgttcag gcgacgcatc
     1741 aaacgttcca gtgcgggaag atcataaaca tcttgcagac attcgcgtag gatttcgaga
     1801 atgaccggaa aatctggata tccctgagcg atttccagca actgactggc gcgcagccgt
     1861 tgttgccaaa gcggggtgcg atggcccgga gtgcgccccg gcattaataa tgcccgcgcg
     1921 gcgcattcgc gaaaacgggc ggcgaaaagt gccgagctgc ctaccgcctc gcggacaatt
     1981 tgcagcaact tttctggttc aaacaaaaaa atcgcggcgt cgggcaattt accatcagtg
     2041 tcaggaatgc gcgcaacaat gccgtcatca ctggcgacca cagacgcgtc agcgccccat
     2101 agggcatgta ttcgcccggc aatcgccagc gcccagggtt catgcacccg tcttccatag
     2161 ggagagtgca aaataatacg ccagtcgcca atttcatcac ggcaccgttc gaggaccaga
     2221 tggcgactcc ccggaacgat gcccgtcgcg ttgcgttgct cgtcaatcaa cccctgaata
     2281 ttggcgttcg tattttcttc tgccagccac gggggaatag tcccggaaaa gaacgcgcca
     2341 tccgccagta aatgaagaaa atcgccgatc atctcgccta attcagccgg acgtccgtta
     2401 ccttcaccac gccagaaggg gagccgggca gaacgacccg gagcaggagt cacaatcacc
     2461 tgatcgcggg tgatttgctg gatccgccat gaggtagcgc cgagagtgat aatgtcgttc
     2521 acccgcgact catataccat ctcctcatcc agttcaccca cccgccgcga accggccttt
     2581 tcttcacctt cgggtaataa cacgctatac atgccacgat ccggaatggt gccgccgctg
     2641 gtaaccgcca acaattgagc gccaggtcgg acggtcaata tcccggtctc cctgttccag
     2701 accagtttgg ggcgaaaagc agaaaaatcg ccagagggat agcgcccgga aagcatatcc
     2761 agcgtggcgt caaaaacacg tcttggcaga tctttccacg gtgcggcacg gcgtacgcgg
     2821 gagtaccatt cgtctacctg taatgcctcc atcgccgcgg cggcaacggt ttgctgcgca
     2881 aggacgtcga gtggattatg cggcggtgtc aggttttcca gcctgcctgc gaacatacac
     2941 tctacaatga ctgcggaatc gactaagtca cgccgggtac gagggaaaaa cagcccttta
     3001 gatacaccgc caacctgatg tccggcgcga ccaatgcgtt gtaacccact ggcaacagaa
     3061 agcggcgttg ccacctgaat caccagatcc actgcgccca tatcaatccc cagttcgaga
     3121 ctggaggttg cgaccacgca gcgtaactca cccgatttca gcgcctgctc ggtgattgct
     3181 cgttgttctt tagagacgga gccgtggtgt gagcgggcaa taaaaacgtc gctactttgt
     3241 acacggttag aggttgcgcc ggaggtcgac tcgaaatggg ccgcatcaac ggcgatagac
     3301 ggggaacgct gtaagcgtgc ggcgtaaagc tcatttaatc gtgccgtcag tttttccgcc
     3361 agcccacgtg aattagtaaa gacaatggtc gagcgatggc gcaacacttc atcaaggata
     3421 cccgtttcaa tatatggcca gatggagcct tcccggccgg catggctgtc ttcgccggtg
     3481 ctgctggcga ccgatgagac atcatccata ttggcgaccg gtacgacaat tcgtatctgc
     3541 ggatggcgca ttgcgggcgg gttgactacc gtaaccgggc gatcgccacc aagaaaggct
     3601 gccacatcgc tggctgagcg cacagtggca gaaaggccaa ttcgctgtgc tgaggtgtgg
     3661 agcagcgcat cgagccgctc cagacttaac gccagatgcg caccacgttt actgcccgct
     3721 accgcgtgga cttcatcaat aattaccgtt tcgccgcgcc gccgccgttc atcggcaata
     3781 cccttcaacg ggagctgcaa gttgcgctga acgtcggtgc ccagagcttt tatcggtgaa
     3841 atatagagga tgcgtgaggt tttacgctta tgcgcctcgc gggtatcttc gccgccttcg
     3901 cggaagagcc gatcgagggc gtagagaaat gctgccagcg ttttcccgga gccggtcggt
     3961 gcaatcacca gcgcatgttc gcttcgcgcc gccacatgcc aggtttgcga ctggacagcg
     4021 gtcggctgtt taaaggcgcg aagaaaccag tcgcgggtcg ccggtgaaaa cacgtccggc
     4081 aggagcgatg aagggtctgg attatctgcc attaacgcta ttccccgtga atatcatgca
     4141 ggatgcgtgg aatagcctca ctatagatgc ctgaatcatt gcatgtcatc aggcatcgac
     4201 tcgattacac ctcttcggcg gcaggtagcg gccagcctcc cagacgtttc cagcggttga
     4261 cgatttcaca aaacagcaca gcagtgcgtt cggtgtcgta cagcgcggag tgcgcctggg
     4321 tgctgtcgaa gtccatgcca gcggtctggc aagcctttga caatacggtt tgtccgagtg
     4381 ccagcccggc cagtgcagca gtgtcaaaag tggcgaaagg gtggaacggg ttacgtttca
     4441 gtgaggcgcg ttctgcggcg gctatcataa agctgtgatc aaaattggca ttgtgcgcca
     4501 ccataatggc gcggttacag ccgctcgctt taataccttt acgtacaact ttaaaaattt
     4561 cgtgcagcgc ctcgtattcg ctgaccgcgc cacgatcggg atcgttcggg tcaatgccgt
     4621 tgaaggcgag ggcttctggt tgcaaatttg cgccgacaaa tggttcgacg tggaaatgta
     4681 atgtggtgtc cggcatcagc cagccttgtt catccatttt cagggtgatg gcggcaatct
     4741 caagcagcgc atcggttttg gcgttaaatc cggctgtttc aacatcgatc acaacaggat
     4801 aaaaaccacg aaaacggtcg cacagaccgg taagttgagc gttatcggac atctgattct
     4861 cttaatacgg ataaattgca gcgcgcatta tgacaaatat tacttgtcga tgcagtaaag
     4921 atgcgggcgc gatgagttca cgcccggcag gagattagtt gcccagaccg cgaccggcgt
     4981 ctttctcttc gattaactca attttgtaac cgtccggatc ttccataaac gcgataaccg
     5041 tagtaccgcc ttttaccgga cccgcttcac gggtcacgtt acccccgttt tgacggattt
     5101 tttcgcacgc ttcagcggcg ttatctacgc taagcgcgat gtgaccataa gcagtgccga
     5161 gttcgtattt atccacgccc cagttgtagg tcagttcaat caccgcttct tcggtttccg
     5221 ggccgtagcc aacaaacgcc agtgagtatt tgtattccgg gttttcgctg gtacgcagca
     5281 gtttcatgcc cagcactttg gtataaaaat cgatggagcg ttgcaaatcg ccaacgcgca
     5341 gcatggtatg aagaagacgc atctttttat cctcaaaatg gctaacagat tcaaaatgtt
     5401 gttttagtat agcggcggct ttacgccgct cgcaatgttg gattacaacg tcgggtaatc
     5461 ggtatagcct tccgcgccgc caccgtagaa actttcggca cgctgtgggt taagctcagc
     5521 tttgcgctgc aagcgggcga ccagatccgg gttcgcaatc cagtcacgac caaatgccac
     5581 cgcatcaatt aaccctttgc cgatcagcgt ttcagctttt tctactgtgt atgcacctgc
     5641 gccgataatc ggaccgtgga aacgggcgcg tactttttcg cggaacgcat cagtatacgg
     5701 ttcacccccc gcccaatctg gttctgacat atgcagataa gcaatgccgc gtttacccag
     5761 ttgttcaatc agatacagtg catcggcttc ttcattcggg ccgttatccg tgttctggaa
     5821 agtaccgatt ggcgaaatgc gaatgccaat gcgatcggca ccccattctt caatcccggc
     5881 atcgaccact tccagtacca gacgtgcgcg attttccacg ctgccgccgt actgatcggt
     5941 acgatggttt gaagaaggag aaaggaactg atgcagcaaa taaccgtgag cagagaggag
     6001 ctctaccaga tcaaaaccgg cttcacgcgc gttagcaatg gcctgacgga aatcattgac
     6061 gatacctgga atctcttcta gttcaagcgc acgcggcatg gatgtttcaa cacggatcgc
     6121 ctgaccattt tcatcgcgca gagaagtacg tgttcccgcg ctaagtgctg aaggcgctac
     6181 cggtgcctga ccgccaggtt gcaggctggc gtgagaaatg cgtccggtgt gccacagcta
     6241 cacggccata tgaccatttt cagcatgaac gccagcggtg atttttttcc atgcggcaat
     6301 ttgctccgga ctatggatgc caggcgcacc tgcatatcct tttacctggg cagaaatttg
     6361 cgtggcttca ctaataatca aaccggcact ggcacgttgg cgatagtatt ccgccatcaa
     6421 cggggtagga atgtcacccg gttcaatact gcgcagacgc gtcagcggtg ccataaaaat
     6481 acggtttgcc gccgtgatcg cgcccacttt cagtggggaa tacagttttt cagatgacat
     6541 aaaggctccc ggtcgtctgg tgataaaaaa tgcctgctaa acggcaggcg tcgcaataat
     6601 gttttttaca tgggccagcg cgttttccag tggctcgaaa ctgcgcgaaa ttttggcctg
     6661 cagattcgca cccagccata gtgcgtaaag cacttgtgcc tgttgtagcg gttcgccaca
     6721 aaaggttaaa caatggttct cacggccatt ttccagcgcc tgagagagca gggcgatcac
     6781 gccgcgagca cctttatcca tcgcgctgcg catatcttct gacagatcgc acacttcggc
     6841 agagagtttt actgtcaggc aaccactgat ggttccatgt tggcaaaact ggttcagtgt
     6901 ttgctggtaa taagccagta tgcggtcgcg gtagttacct tcgccggatt gcagcaactc
     6961 agtcagtcgc tggtgatatg cggtgtaatg acgctcaagc atggcaacgc caaacgcttc
     7021 tttagagcga aagtagtgat agaaggaccc tttcggcact tcagcggttt ttagtaattc
     7081 gcttagcccc atcccggtga atccacgttg caggcaaagt tgctcgcccg tcgccaggag
     7141 atgttcgcga gtatcatgtt cggtgtgttt gttcatgcgt tggagtgtag tagaccagtc
     7201 ggtctagtgc aagcaaagtt gctactttgg acattaacag cttcaataat ggtaataatc
     7261 ttatctgagg agggcgaagt ggcggagcaa ttagagttct ttcccgtcca gagcccgtgc
     7321 cggggaattt gccagtctga cgaacgcggg ttttgtcgcg gctgttttcg tagtcgggat
     7381 gagcgtttta actggaataa aatgagcgat ggcgaaaaac aagaggtgct gcgcctttgt
     7441 cggcagcgat taatgcgcaa attacgcgca aataagccag cgtcatcaga cgaaccggag
     7501 caaccatcac tcttttgaac ctaatcttgc gtatactcct ctcatatctg ataagaggaa
     7561 gcgattatgg ttcagcgtat tactattgcg ccgcaaggcc cggagttttc ccgttttgtg
     7621 atgggctact ggcgattgat ggactggaat atgtccgccc gccagctggt cagttttatt
     7681 gaagagcatc tggatctcgg cgtgaccacc gtggaccatg ctgatattta tggtggctat
     7741 cagtgcgaag cggcgtttgg cgaggcactg aaactggcac ctcacctgcg tgaacggatg
     7801 gaaatcgtca gtaaatgcgg tatcgcgacg accgcgcgtg aagaaaacgt cattggtcat
     7861 tacatcactg accgcgatca catcattaag agcgccgaac agtcgctaat taatctcgcg
     7921 accgatcatc tggatctgct gttaatccac cgaccagacc cgttaatgga tgccgatgaa
     7981 gtggcggacg cgttcaaaca tctgcatcag agcggcaaag tgcgtcattt tggcgtatcg
     8041 aactttacgc ctgcgcaatt tgccctgttg caatcacgtc tgccgtttac ccttgccact
     8101 aatcaggtgg aaatatcccc ggtgcatcag ccgttactgc tggatggcac gctcgaccaa
     8161 ctacaacaac tgcgtgttcg tccgatggcg tggtcctgcc ttggtggtgg tcgtctgttt
     8221 aatgatgatt atttccagcc gctgcgtgat gaactggctg tggtggcaga ggagttaaac
     8281 gcgggctcga ttgaacaggt ggtttacgcc tgggtattac gtttaccatc gcagccgctg
     8341 ccaattatcg gttcaggtaa aattgagcgc gtacgggcag ctgtcgaagc agaaacactg
     8401 aaaatgaccc gtcaacaatg gtttcgtatt cgtaaagcgg cactggggta cgacgtaccg
     8461 taagcacttt taggaatagc cgccgttcaa aaatgtgtca ctggtttaca cttattcagg
     8521 aatgcacaat gaacggaggt cctatgaaac gttttagtct ggctattctg gcgctggttg
     8581 ttgcaaccgg cgcacaagct gccagtgaaa aagtcgagat gaacctcgtc acgtcgcaag
     8641 gggtagggca gtcaattggt agcgtcacca ttactgaaac cgataaaggt ctggagtttt
     8701 cgcccgatct gaaagcatta ccccccggtg aacatggctt ccatattcat gccaaaggaa
     8761 gctgccagcc agccaccaaa gatggcaaag ccagcgccgc ggaatccgca ggcgggcatc
     8821 ttgatccaca aaataccggt aaacatgaag ggccagaagg tgccgggcat ttaggcgatc
     8881 tgcctgcact ggtcgtcaat aatgacggca aagctaccga tgccgtcatc acgcctcgtc
     8941 tgaaatcact ggatgaaatc aaagacaaag cgctgatggt ccacgttggc ggcgataata
     9001 tgtccgatca acctaaaccg ctgggcggtg gcggtgaacg ctatgcctgt ggtgtaatta
     9061 agtaattaag aggcctgcgt accttgcggt ggtgcttgct caagttgcga aagcgagcag
     9121 tacagccgcc agacaattgc cgccagctca cgggcggcag gttgatgatg acgggcaagg
     9181 ctgtcgcaaa tccgctgtaa ttcttcaagt gtggccgcca gtgatttttg ctgaacgcca
     9241 cgctcactca tcactccccg caacagtgaa atacagttat cccgtactcg cgataacgga
     9301 tcggaacgcg attcccagtc gcgcaattgc cagacaacat gagaacagtt cagcagcact
     9361 acaccccagc gtaataacca acggcgagcc agcgcatcct ggctgttact caactgactg
     9421 acgtgatgat aagtgagcga ttcaaattcg ctttcactca gtgttggatg gcggcttagc
     9481 tgatcgacaa aatcccggcg cagcgcgcga atatggcggc ggcttttacg agcatccgat
     9541 cctggacgca gaatggcgaa cgctagccaa gccagcgcaa cgccaacgat ttttgccaga
     9601 ttatcgttaa gaaagtcagc aaaatcatac accgggggat tagtgacagc gataaacgaa
     9661 cccataaaaa caatcagttg cccccacagt gcggcaaatt tgggcatctg caatttaaga
     9721 agctgcattg tcgccagcag tggaaagaga aacagtaaaa attgccacag atcgctaatc
     9781 tggaccatca ggccaaattt gaccacaaag ctgaatagcg aaagtaacac cagcgtgcgc
     9841 atcagcagtg acaacgactt aaacggtgct gcgacggcgg agtagagtac gcagctaatt
     9901 gctgccagcg ttaatgcatt ggcaccggca tcctattgcg atgcaatact ccatgcgcca
     9961 atcatcatca acgtacaaaa tgtacgcagc ccgctccaca tagcttcggc gttgtcggta
    10021 tgacgagcca gagcggatgt ccgtgcgagt ctggcatgat catctacacc gctttgcaga
    10081 cgatgtaatt cctgactact ttgcagataa aggcggcaaa aataacgtag tcgctgccag
    10141 aaggcgacgt gccggtagtc ggcgacgttg gtcgggcgta gcggggcgat aatacgtgcg
    10201 acggtgtaag catctgtttg cgaactggcg agcgccgtca gcaattgttc gagaatttct
    10261 cgcgtggcac ctggcggtga gggccagttg agcaacatac gtcgcaggct ggagatgaca
    10321 ctggtcatac gtaattgctg gtggagtagc gcattaaggc gcgcgttttg ctggcgaaaa
    10381 cgatagtggc tccagaaagc ctggatacgc agcaaattca tggtcagtat ctgcccaatc
    10441 cccccctcat gtgctgcacg aatggcatcg tttgtttcag gctgccagag taaactggca
    10501 tgttccagta atcgggcgtg catgtttttc aatgcggtta aaagggctgt agcatcggaa
    10561 ctgctaggca ggatcatcat catcatgccg ccgcacaaaa taccgactat cacctcgcaa
    10621 acgcgcgcct gagcgatatc ctacagctgg ctggcctcag taatattaac catcggaaag
    10681 gcgatgatgg cagccgtgta gccgccagtt gaaatgcata cgcgacgtta ttcgtgaagt
    10741 gcgcacaggc ccaggtacaa aagccaagcc acgccgacat gctcaataga aaaaaccacg
    10801 gctcattgag cgtatgcccg gcaagaagca gtgccgcaat ggctcccagt aaactgccag
    10861 cgatgcgtcc gaggcttttg ctgatgacac cgccaacggt gggaaagcta accactgcag
    10921 ccgaggtcat cgcccaatag ggttcatcca gattcaaata ataggcaacc gtcagcgcca
    10981 gacacatggc aatggtattg cgtaacgcat aacgccattg cgccagcgtg gccttgaacc
    11041 agggcaaatt gcgcaaggac catgacgatg cgttcattat tgacctacgg caatgctaca
    11101 ggtggtgccc gacaccagcg tgacatcgcc tggaacttta tccagcgcaa agcggacggg
    11161 aacgcgttgg gcgagacgaa cccaggggac gttaggtttc acatccggaa taaggcttga
    11221 gtcactttca acgctttgat cataaatcgc ccgaccgata ctggaaacgt gaccccgtaa
    11281 cgttttgtta tcactataga gggtaatttg tgctggtgca ccttcgcgga tatggcgcaa
    11341 cttggtttct tcaaaataac caatgacgta aaacgagtgg ctgtcgacaa gggcaaacag
    11401 cggtttcccg gtgtcagcgt aatcgccgat gcgggtagtg aggttagtca cccaaccact
    11461 caccggggcg cgaatttccg tttgcgccag ttgccattgt gtctgtttca gcgtggcctg
    11521 tgcggcatta acactggcct gcatcgcttt aacattgagg ttagcggtat cgagttcttc
    11581 ggcagaaata aaattttgtg agagatgacg acgacgattc gcttcgttat tggcttttgc
    11641 gaggtcagat tgcgctttcg ccagctgagc ctgtgcgttc agctcggcga tctgaaaggg
    11701 cgttttatcg atcgtgagca gaagatcccc cgcgttaacc agttggttgt cttttatatt
    11761 cagctcaaca atacgtcccg acacctgtgg ggtgatagaa accttttctg cgcgtatttt
    11821 cccgtcgcgt gtccacggag attgcatgta ataattccac agccaccatc cggcaagaac
    11881 cgcaactatc gctacaataa ttattgagaa atacttaatt gttttaattg acatagttac
    11941 cacgcaatca gtattgccag agcaaggcaa acgcaaatcg caaacagcga taaatccatt
    12001 tacaagggat gccagatgtc accggcgtag atccagccac gaagcaggcg gtgtacgaca
    12061 agccagatga cgaatccaaa cgcgaaggct ttgaaaaacg gaggaaaata gacggacgca
    12121 ccgaacacca gatcttgtaa gggcaatcct gttgcattga gcataaactt cacaaagtag
    12181 attcctttac gaccgaatat attgcgttag attaataaat attctttaag tgcgaaaaat
    12241 ttacgcgcaa tttctgaaaa atatgtctga cggtaaccaa atgcagcaat acatttgttt
    12301 tagcaataaa attgctgcac actattctaa aagccgcata atatcttagc aagctaatta
    12361 taaggagatg aaattggaat cgccactagg ttctgatctg gcacggttgg tgcgcatatg
    12421 gcgtgctctg atagaccatc gcctgaaacc gctggagtta acacaaaccc attgggttac
    12481 gttacacaat atccatcagt tacctccaga ccagtcgcaa attcaactgg caaaagcgat
    12541 tggcatcgag cagccatcac tggtccgtac tctggaccaa ctggaagaaa aagggttaat
    12601 ttcgcgtcaa acttgtgcca gcgatcgtcg ggctaaacgt attaaactga cggaaaaggc
    12661 agagccactg atcagcgaaa tggaagctgt tattaacaaa acccgcgcgg aaatattaca
    12721 tggcatctcc gcagaggaac tggagcaact gattacgctc atcgcaaaac ttgagcataa
    12781 tatcattgag ttacaggcca aagggtgaaa tgaagggggc gtgtggtcag taacctgacc
    12841 acacgcaaac ttatttagcg cggagaaacg gtcacctgac tgccattgct ggccagtacg
    12901 acacgttggc ccggagagaa acgagtgttg ccttgtttct gtaccaccat gatggtatta
    12961 ccatcgtctt tacgaatttc cagctcgaca ccctgcgttt tgttcattgc actctgtacg
    13021 ccctgaccag ctacgccacc tgcaacagcg cctgctgcag tagccagaga acgcccggtt
    13081 ccgccaccaa cagtattccc caggaaacca ccaagaacag caccgccaat tgcaccgata
    13141 acgttggaat catcaccgcc ctgaatctgt accggacgta cgttaacgat ggtgccatag
    13201 ctgacattct gtacttgttt cgcttcagaa gcggtataaa catcccctga cagggtgtcg
    13261 ttattaacac aaccgacaag agacagacct accattgaaa caaccaatac gcgtttaatc
    13321 attgaaaaat ctcctgttca ccatgaaacg ccacgcaagc atccctcatg gtcaaagtat
    13381 atggcatatg tgaatccatg gttacatgtt ctaccaaata tgagcaaatc ataatgaaag
    13441 taggatcaac caattataaa caaaacattc atgatgatta ttgatttgaa gataatgttt
    13501 tactaccaac ggtaaaaatc ggtcattccg catggtattc acgaaaagtt tatgtttgag
    13561 tggtgcgcaa acgtcagatt gaaaaaggat gaactatgaa atcgggccgc tttattggcg
    13621 ttatgtcagg caccagcctt gatggtgttg atgttgtgtt ggcgacaata gatgaacacc
    13681 gggtcgcaca gctggcaagc ttgagctggc cgatcccggt gtctctgaaa caggctgtac
    13741 tggatatttg ccagggccag cagcttacac tttcgcagtt tggacagctt gatactcaac
    13801 tcgggcgact ttttgctgat gcggtcaatg ccttgcttaa ggaacaaaac ctgcaggcga
    13861 gagatatagt tgcgatcggc tgtcacggtc aaaccgtctg gcatgaaccg acgggcgtgg
    13921 caccacacac tttacagatt ggcgataaca atcaaattgt ggcacgcacc ggaattacgg
    13981 ttgtcggtga ttttcgccgt cgcgatattg ccttgggagg acaaggcgca ccgctggtac
    14041 ctgcgttcca tcatgccttg ctggctcacc caaccgagcg acgaatggtg ctcaatattg
    14101 gcggcatcgc caatctgtca ctgctcattc ctgggcagcc ggttggcggc tacgataccg
    14161 gtcctggtaa catgctgatg gatgcctgga tctggcgtca ggccggtaaa ccttacgata
    14221 aagatgccga gtgggcacgg gcgggtaaag ttattctccc actgctgcaa aatatgctca
    14281 gcgacccata tttctcgcaa cctgcaccga aaagcaccgg acgcgaatac tttaactacg
    14341 gctggctgga gcgccattta cgccattttc cgggtgttga tccccgtgat gttcaggcca
    14401 cactggcaga actcaccgcc gtgaccattt ctgaacaagt tttgttgagc ggtggctgcg
    14461 aacgattgat ggtatgtggt ggaggtagtc gtaatccgct actcatggcg cgtctggcgg
    14521 cattactgcc aggcacagaa gtcaccacca ccgatgccgt tggcattagt ggcgatgaca
    14581 tggaagcatt ggctttcgcc tggcttgcct ggcggacgct ggcgggatta ccaggaaatc
    14641 tgccttccgt cactggcgca agccaggaga cggtactggg ggctattttc cctgctaacc
    14701 cgtgatatca gagttaactg aatttttctt accgcgtctg ccgttacact tggaacgtta
    14761 gggagggcgt attgccctcc agaccaggaa agtcttcggg aaatgtctat gaaaaaactg
    14821 ttactgatct gtttgccggt gcttctcacc ggctgtagcg cgttcaatca actggttgag
    14881 cacatgcaaa ccgctacgct ggaataccag tgtgatgaaa aaccgttgac ggtcaaactg
    14941 aataatccgc gccaggaagt cagttttgtt tacgataatc aactactgca tctcaaacag
    15001 ggcatttcag cgtctggtgc acgttacact gacggaatct atgttttctg gtcgaaaggc
    15061 gatgaagcga ctgtctataa acgcgaccgc atcgtattga ataactgtca gttacaaaat
    15121 ccacagcgtt gagatttttc caggggcggc gcacaatagc gtcacccact gacaatccgt
    15181 aaagaaaacc atgtctgata acgacgaatt gcagcaaatc gcgcatctgc gccgtgaata
    15241 caccaaaggc gggttacgcc gtcgcgatct tcccgccgat ccattaaccc tttttgaacg
    15301 ttggctctct caggcgtgtg aagccaaact ggcagaccct accgcgatgg tggtcgctac
    15361 cgtggatgaa catgctcagc cttatcaacg catcgtttta ctcaaacatt acgacgaaaa
    15421 aggcatggtg ttttacacca acctcggtag ccgtaaagca catcaaattg aaaataatcc
    15481 gcgcgttagc ctgctgttcc cgtggcatac ccttgaacgc caggtgatgg tgatcggtaa
    15541 agcagaacga ctttcgactc tcgaagtgat gaaatatttt catagccgcc cgcgtgatag
    15601 ccagattggt gcatgggttt cgaagcagtc cagtcgcatt tctgcccgcg gtatccttga
    15661 aagtaaattc ctggagctga agcagaagtt tcaacagggc gaagtgccat tgccgagctt
    15721 ttggggcggt tttcgcgtca gccttgaaca gattgagttc tggcagggcg gtgagcatcg
    15781 cctgcatgac cgctttttgt accagcgaga aaatgatgcg tggaagattg atcgtcttgc
    15841 accctgaaaa gatgcaaaaa tcttgcttta atcgctggta ctcctgattc tggcacttta
    15901 ttctatgtct ctttcgcatc tggcgaaaag tcgtgtaccg gcaaaggtgc agtcgttata
    15961 tacatggaga ttttgatggc aagcagtaac ttgattaaac aattgcaaga gcgggggctg
    16021 gtagcccagg tgacggacga ggaagcgtta gcagagcgac tggcgcaagg cccgatcgcg
    16081 ctctattgcg gcttcgatcc taccgctgac agcttgcatt tggggcatct tgttccattg
    16141 ttatgcctga aacgcttcca acaggcgggc cacaagccgg ttgcgctggt aggcggcgcg
    16201 acgggtctga ttggcgaccc gagcttcaaa gctgccgagc gtaagctgaa caccgaagaa
    16261 actgttcagg agtgggtgga caaaatccgt aagcaggttg ccccgttcct cgatttcgac
    16321 tgtggagaaa actctgctat cgcggcgaac aactatgact ggttcggcaa tatgaatgtg
    16381 ctgaccttcc tgcgcgatat tggcaaacac ttctccgtta accagatgat caacaaagaa
    16441 gcggttaagc agcgtctcaa ccgtgaagat caggggattt cgttcactga gttttcctac
    16501 aacctgttgc agggttatga cttcgcctgt ctgaacaaac agtacggtgt ggtgctgcaa
    16561 attggtggtt ctgaccagtg gggtaacatc acttctggta tcgacctgac ccgtcgtctg
    16621 catcagaatc aggtgtttgg cctgaccgtt ccgctgatca ctaaagcaga tggcaccaaa
    16681 tttggtaaaa ctgaaggcgg cgcagtctgg ttggatccga agaaaaccag cccgtacaaa
    16741 ttctaccagt tctggatcaa cactgcggat gccgacgttt accgcttcct gaagttcttc
    16801 acctttatga gcattgaaga gatcaacgcc ctggaagaag aagataaaaa cagcggtaaa
    16861 gcaccgcgcg cccagtatgt actggcggag caggtgactc gtctggttca cggtgaagaa
    16921 ggtttacagg cggcaaaacg tattaccgaa tgcctgttca gcggttcttt gagtgcgctg
    16981 agtgaagcgg acttcgaaca gctggcgcag gacggcgtac cgatggttga gatggaaaag
    17041 ggcgcagacc tgatgcaggc gctggtcgat tctgaactgc aaccttcccg tggtcaggcg
    17101 cgtaaaacta tcgcctccaa tgccatcacc attaacggtg aaaaacagtc cgatcctgaa
    17161 tacttcttta aagaagaaga tcgtctgttt ggtcgtttta ccttactgcg tcgcggcaaa
    17221 aagaattact gtctgatttg ctggaaataa tgcattaagt ggaaaggggg agtgagaaat
    17281 cactccccct ggtttttata cagggaacat gatgaaaaat attctcgcta tccagtctca
    17341 cgttgtttat ggtcatgcgg gtaacagtgc ggcagagttt cctatgcgcc gcctgggcgc
    17401 gaacgtctgg ccgctgaaca ccgttcaatt ttctaatcac acccagtacg gcaaatggac
    17461 tggctgcgtg atgccgccca gccatttaac cgaaattgtg caaggcattg ccgccattga
    17521 taaattacac acctgtgatg ccgtgttaag tggctatctg ggatcggcgg agcagggtga
    17581 acatatcctc ggtatcgtcc gtcaggtgaa agccgcgaat ccgcaggcga aatatttttg
    17641 cgatccggta atgggtcatc cggaaaaagg ctgtatcgtt gcaccgggtg tcgcagagtt
    17701 tcatgtgcgg cacggtttgc ctgccagcga tatcattgcg ccgaatctgg ttgagctgga
    17761 aatactctgt gagcatgcgg taaataacgt cgaagaagcg gttctggcag cgcgcgaact
    17821 cattgcgcaa gggccacaaa ttgtgttggt taaacacctg gcgcgagctg gctacagccg
    17881 tgaccgtttt gaaatgctgc tggtcaccgc cgatgaagcc tggcatatca gccgtccgct
    17941 ggtggatttt ggtatgcgcc agccggtagg tgttggtgat gtgacgagcg gtttactgct
    18001 ggtgaaactg cttcaggggg caacgctgca ggaggcgctg gaacatgtga ccgctgcagt
    18061 ctacgaaatc atggtgacca ccaaagcaat gcaggaatat gagctgcaag tggtggctgc
    18121 tcaggatcgt attgccaaac cagaacatta cttcagcgca acaaagctct gaaatatctc
    18181 atttcggccc acaacgacct gtgggccgat ggctattact ttaagccttc cgctgacagt
    18241 gcgtcttgta cttccggacg ttcagccata cgttgcataa atgctgcaat gtgctctaac
    18301 ccttccagat tcagtttcac cgcgtatgcc cagcgcagaa ccgtaaacag ataggcatca
    18361 gcaattgtaa atctttgccc gcagatccag tgctcatcct tcagtgtctc gttcacatat
    18421 tgcagcttct tctccagctg cgcgcgaact gtcggtttgt actcttccgg tgtatcaggg
    18481 cgaaacagag gtgtgaaacc tttatgcagc tcggtggcga tgtaattcag ccattcgatg
    18541 gttttatagc gggaaatact gtttaccggt gccagcaact ggcggtcggg gacgctgtcg
    18601 gcaagatact gcataatcgc tacgccttcc gtcagcaaag taccgtcatc cagcagcaat
    18661 gcaggcacct gccccttagg gttaacggca aagtaatcgt caccgttttc gagacgtttt
    18721 ttcattaaat ccacactgac gagggtaaaa tcctttccgc tctcacgcag ggtgatatgg
    18781 gaagcgagag agcaggcacc cggtttgtag aacaatttca tcggtaactc cttttgtctg
    18841 tggtttaaag tatggtagtg cgttgctgaa caaaaaaaaa gccgctaacg aaaagttagc
    18901 ggcttgtaaa agagtttccc taaattttac gctacagcag ctttcgccgc tttgtctgca
    18961 gcgtcatcct gcgtcatacg gtgcagttta ggcgcggtca gcagcatcag tactgcaata
    19021 atggcagtag cgacaccaat ctgcaagaat acgcgaccat aaacttccag tgacatcagc
    19081 gggtcggtaa cgttatccgg cacagccatc atacccgcaa cataaccacc aatcaggttt
    19141 gcaccggcag tggtcaggaa ccagctaccc ataatgaagc ccatcagacg ctgcggaacg
    19201 agttgtgcaa ccattgccag acccagacca gagatcatca gttccccgat gctctgcagg
    19261 ccatagcttg cgaccagcca gcttacagac acgataccag cgtcagacgc gaatttcgca
    19321 cccagcggca gaatcaggaa cgcaccagaa cacatcacca tgccgattgc aaacttggtt
    19381 ggcatcggca gggtatcgcc catcttgtta tagatagcgg ccagaatcgg actaccgatg
    19441 atgatccaga acgggttcag tgcctgatac tgctcaggtt ctacggccag acccagaatg
    19501 gagtgctcaa cgttacgaat cgcaaagaag ttcagtgacg ttggcatctg gctgtacagc
    19561 acgaagaaga taatggcttc gagcatcagg atgaaggcaa cgatcatttt acgacgcgca
    19621 gcacctttca tcgcgaaggc ttctttaccg aagataacca cgataccgaa ggcaacaacg
    19681 cccagcgcca tacgcgcaac ttcctgattg tgcagcagcc aggtggcgat agcgatcagt
    19741 gccacaacac caataatggt cagtagcagg ttacggtagt tgataggctc gaagtctggt
    19801 tttgaaccgt actgtttaac ccagcgttgg cagaaggcga agttaacgat agtgatcagc
    19861 aggcctacaa cgctcaacgc aaacgcaaca ctccagccgt atttcgcggc cagccacggc
    19921 gtagcaatca tagagaagaa agagccgatg ttgacggaca tgtagtacat ggtgaatgca
    19981 ccgtccagac gcgggtcgtt tttctcatag catgtagaaa gcagagaaga cgggttagct
    20041 ttaaacaggc cgttaccgac cgcaatagcc gccataccca tataaacgat accggcgtcg
    20101 tgaccagacc aggcaaccag agcataacca atcgccagca caatagcgcc gagcataatt
    20161 acgcgtttag tacccagtac cttgtcacct aaccagccgc cgatagcgac cagaccataa
    20221 accagggcac taaaggaaga gaaaagggtg attgaatccg cttcagacat acccagttgt
    20281 ttaaccaggt aaacagccat aattccttgt aggccgtaat aaccaaaacg ttcccataac
    20341 tcaatcgaga agatgagata gaacgccttc ggttgtttga aagcgttcaa actgacgctt
    20401 tcagttggtt tttggtttgc agtggacact tttacctctt tattttacat cccatattaa
    20461 cgggggtgct ttctcgcgtt atgtctaaaa aacatacgcg caattgttat agtgggaggg
    20521 gaaacggcag gtaatgttca ctatcttgca ctttcagaca agtcctttgt aataatctgt
    20581 tacatataac tgaggcgggg aattcaacca ttccgggaaa tgttatccag cgttaaattt
    20641 ttcaaaaaac gtaacggcag attttttcac tgcactgaga ggggggtgat ggcttaacgg
    20701 cgatatgttc tgctatttag cgtttttact agtgagatag tccagtttct gaaaaatagc
    20761 cagtgtaatg ttttgtaggt caatatttgt tgcgtgtgta aatttaaatg attttaacct
    20821 gattcttgca atgtttttac attgcgacag catcgtgatc ttgatcacga atgaatagaa
    20881 aatttcgcta ttaaaaaaac aattaacctg catttttggt tatccgcaaa cggattacct
    20941 gatgataacg ctaagcaatg gcatcattca cgttaaaaag atgttttaaa agaggataaa
    21001 gaaaggttat caatggggta atcggtgtta ccccttttct tcagatgtca actttctctt
    21061 tgtattcaca aagatcttca ataatacaag agccacagcg gggcttgcgg gcaatgcagg
    21121 tataacgccc gtgcaggatc aaccaatggt ggcagtcgac tttaaactct gccggaacca
    21181 ctttcagtag cttttcttct acctgttcaa cgtttttccc cggcgcaaat tgagtacgat
    21241 tacaaacgcg gaaaatgtgc gtgtcgacag caatagtcgg ccagccgaat gcagtgttta
    21301 atacgacgtt ggctgtttta cgacctacgc cgggcagggc ttcaagcgca gcacgatctt
    21361 ccggaacctc gccattatgc tgctccagta agatacggca ggttttgatg atattttctg
    21421 ctttgctgtt ataaagccca atcgttttga tataggtttt aaccccttca acgcccagtt
    21481 caagcatcgc tgcaggcgta ttcgccaccg ggtagagttt cgccgtcgcc ttattaacac
    21541 tgacatcggt cgcctgagcg gaaagcagta cggcaatcag caattcaaaa ggcgaactga
    21601 aattaagctc ggtggtggga tgaggattgt tctcacgcag gcgagtgagg atctccaggc
    21661 gttttgcttt gttcatcaga cattccctgt ttcaccgttt ggcaatgcac gttcggcagc
    21721 tgcttcagta cggcgctttt tcattctttc atcaatcagg tattttcctg ccagcatcag
    21781 tcccaggcca ataaatgcac ctggtggcag catcgccagc aggaaagggg agtcggtgtg
    21841 gaaaatctcc acgcgtaata cttttgccca gctacctaac agcgcatctg caccgtcaaa
    21901 caatgtgcca ttgccgataa tttcgcgtag tgaacccagc acgaacatgg cgcaggttgc
    21961 gcccatacca attgaaaagc cgtccagtgc cgaaagcgcc ggaccttttt tggcggcgaa
    22021 ggcttcagcg cggcccacaa cgatacagtt agtgacaatc agcggaataa aaatccctaa
    22081 tgattgatac aggccaaagg cgtaggcgtt gatcagcatc tgtacagcgc tgaccaccga
    22141 ggcgatgatc atcacgtaaa tgggaatgcg gatctcggct ggcgtccagt gacgcagcgt
    22201 cgaaatggtc aggttggtca gcgtcagtac cagcgtagtc gcaagtccta aacccagagc
    22261 attagtggca gtggacgtga ccgccaacag aggacaaagg ccgagcaact ggaccagcgc
    22321 agagttgttt ttccacaacc cctgaacaat aacgtcttta atttcgctca cgctttattc
    22381 tccacaggca ggaagttgag aaagttgtgc cggtaacgtc tgagcgtaca atccggcgcg
    22441 ttttaccgca ttaaccaccg cgcggggagt aatcgtcgcg ccggtgaact ggtcgaaatc
    22501 accaccatct ttcttcacct cccagtgcgc atcatccgca ccactgattt ttttacccgc
    22561 aaaatgggtg atccagtcag aaaggcgcag ttcgatttta tcgccaagcc ctggcgtttc
    22621 gtggtgctct gtcacgcgcg tgccaagtac cgtgccgtta aaatcggctc ccaccagcag
    22681 ctgaatcgca ccggaatagc catctggcgc ggttgcttcc agaacggcgg ctaccggttt
    22741 gtcatcctgt ttggcgatgt aaacccgatg ctcaccttta cctaactctg gcgcagttac
    22801 cagatagcaa ctctgtgcca gcgcattgtt atagcgttcg gctggcagca cctgatcaaa
    22861 taatgccttt tgttgcagac ttgcctgttc agcaatcgtc gttttggtca tctggttgat
    22921 ggccgcagtt aaccctgttg aacccgctgc aaatagcgct aacgtaatgc cgtgttttcg
    22981 gatagttttc agcatggttt accctttgcg atggccgtag acgcgcggac gcgtgtagta
    23041 atcgatcaga ggaaccgtga tgttcgccag caggacggca aaagccacgc cgtcaggata
    23101 gccgccgaaa ctgcggatca accagactaa taagcccgca agcgcgccga aaataagacg
    23161 accacgattg gtcgtagaag cggtaaccgg gtcagtcaaa ataaagaatg cgccgagcat
    23221 ggtcgcacca gacagcagat gaatttgcgg tgctgccagt gtttctggtg agaacaacca
    23281 gcccaacgtt gcgcataacg ccagcgttac taagaagctg aggggaatat gccagcgaat
    23341 cgctttctgc catagcaacc acaggccgcc agccagccag gcgagattta cccattgcca
    23401 accagcgccc gccagaatac cgctgtagat cggatattgc ataatctgtt caaccgaatg
    23461 accggcacgg acagaggttt taaatgtatc cagcggtgtc gcctgactaa tgccatcaat
    23521 acctaagtgt agtgtgttca tatcagcacc actggcggta tgcccgctga aaataacctg
    23581 tatggcgtcg ataaaaccag ggatgttgac cgcaatttca tgtggcggta accagctggt
    23641 catctgcacc gggaaggaga tcagtaagac cacataacca atcattgccg gattaaacgg
    23701 gttttgcccc agaccgccat acaactgttt agcgatgatc accgcaaaca ccgtacccag
    23761 cacgaccatc caccatggcg cgaggggggg aatacttacc gccagcaata agcctgtcag
    23821 caatgctgag ttatctttca acgttgcggc taccgactgc ttgcgtagtt tgagtacgag
    23881 agcttcggct aacagagtac taaccgatgc caacaggatc tgaacgagag taccccaacc
    23941 aaaaaaccac agttgcgctg cgattcctgg cacggctgcg agcaacacca acagcataat
    24001 gcgcgatgtc tggcgctggt tatgggtata aggggagcta gctattctga ataccattta
    24061 gtcctcgttt acaacctttt gctgggcggc ttttttggcc tgagcgcggg caatagccgc
    24121 ggcaactgcc gctttgcgcg gatcaacctg ttcttctggt tctgcgttag tctgttgctg
    24181 ttccagcttg cgcgctttgg cacgggcaat agcggcttcg acggcggctt tgcgcggatc
    24241 aacctgttct tcaggtaccg cattagcctg ttgctgttcc agcttgcgtg ctttggcgcg
    24301 tgcaatagcc gcttcgacgg cagctttgcg cggatcaacc tgttcttctg gctccgcatt
    24361 agcctgttgc tgttccagtt tgcgcgcttt ggctcgggca atagcggctt cgatggcggc
    24421 tttgcgcgga tcaacctgtt cttctggttc cgcattagcc tgttgctgtt ccagcttgcg
    24481 cgctttggca cgggcaatag cggcttcgac ggcggctttg cgcggatcga cctgttcttc
    24541 tggttccgca ttagcctgtt gctgttccag cttgcgcgct ttggcgcggg cgatagctgc
    24601 ttcaacggca gttttacgtg gatcagcaac ggttgctgcg tcgttagttt gctgcagttc
    24661 tgcctgtttc gctctggctt gcgctttacg ggcttcccgt gctgcaataa ttgcactgtt
    24721 atccgggcgt tcgcccgctt taatcacaat aggctgtgta gcctgggcct gtttctcttt
    24781 cacccgcgcc agagcggcag caatcgcatc tttatctttg gctgcaggtt gaacggctgc
    24841 gctcttatgt cgttcaaggc gagccgcttt ttcgcgctcc agacgagcct ggcgcgcttc
    24901 gaaacgcgct ttggcttctg cggcgcgctt ttcttcctga cgaatagccg caatttcagc
    24961 tttttcctga cggaaatatt gcaccagggg aatattgctc gggcaaaccc acgcgcaagc
    25021 cccacattca atgcaatcag caatgttatg cgtggtagct ttatcgtgtt gctgaccttt
    25081 gctgaaccag tacaactgtt gcggcaaaag atccgcaggg caggcgtcag cacaggcgct
    25141 acaccggatg cagctttgtt cttcctgtgg ttcgccaagt tcattggcag agggagccaa
    25201 cagacagttg gtaatcttta cgaccgggac atccagccat ggcaaggtaa agcccattag
    25261 cgggccaccc ataatcacca tttgatcggc agaggggcag aatcgggcat cattcaataa
    25321 atgacgcact ggcgtcccca gccgtgccca gacgttgccc gggcgagcga ttgcttcgcc
    25381 agtcagggtt acaacacgct cggtaatcgg ctcgccatca ataacggcac gtttcactgc
    25441 ataagcagtg ccgacgtttt gcattaatac gccgatatcg gatgaacgcc cgccatgtgg
    25501 aacctgcttc ccggtcagaa tgtaggttaa ttgtttagca ccgccagaag gatatttggt
    25561 tggaatcacc cgcagagaaa tatcgttaga gtccgccagc accgcgcgca gcatggaaat
    25621 cgcctgcggt ttgttatctt caatgccgat aagaatttcg cgtggctgca gaatatgcgc
    25681 aagaatgcga ataccctcta cgacctgagc cgcgcaatcc tgcatcaaac ggtcatcggc
    25741 ggtaatgtac ggctcgcact cagccgcgtt gataatcaac gtttcaatct tatctccgcc
    25801 accctgcaat ttaacgcctg tcgggaatcc tgcaccgccc agcccggcaa caccaaactg
    25861 atgtatgcgc tcgattaact cttcgcgact acgagtgcga taatcggccc agccgtcgcg
    25921 cgggatccag cagtcttcac catcggcatc aataatcacg cttaattcag ctaaagctga
    25981 aggatgagcc gtagagtggg gcgcaatagc cgtaacggta cccgaggtgg gcgcgtgaac
    26041 aggcagcatt ttgccgcgac cacgggtaag cggctggccg cgcaatactt tatcgccgac
    26101 gctaacgcac aactcacctt cagcgccaat atgctgtttc agtggaataa caaaacgctg
    26161 cgccagtggt acctggcgca ggggggtacc gttggactgg gttttcatct ccggtggatg
    26221 gatgccgccg ttgaaatccc agattttatt ttttctgaat gcagagaata acttaagcat
    26281 ggtgttccac gggaatgata cgcacgggaa tggtgttcag atcccatttc caggagtcag
    26341 gtgtttctgc gaccggttgc aacgagatgc agtgcgtcgg gcacggatca acacataaat
    26401 tgcagcccgt acagagatca ctcattaccg tatgcatggc acgggtagcg ccaacgatgg
    26461 cgtctaccgg acaagcctga atacatttag tgcagccaat acagttattt tcatcaataa
    26521 ccgccaccat ccgcgcaggc gttagctctt gcgcttcgcc atccagcggc tgcggctcga
    26581 cattaagcaa ctcggcaatt tttagcatca cagcttcgcc acctggggcg caacggttga
    26641 ttttttcacc gttacagctg atggcttccg cgtaggggcg acagccggga taaccgcact
    26701 gaccacactg gctctgcggt aagatttcgt caattttctc aacgaccgga tcgtcttcca
    26761 ccgcaaaacg gcgggaggca taacccagaa tggcgccaaa cgccaggccc agcaggctca
    26821 cggcggcaac ggcaatccag atagcattca ttacaacttc accaaaccac taaagcccat
    26881 aaaggccaga gacataagac ctgcggtaat taacgcaatg gcattaccgc gaaaaggtgc
    26941 cgggacatca gccacagcaa ggcgttcgcg gatggcggtg aagagcacca tcaccagcga
    27001 gaaaccgaca gcggcggaaa aaccgtacag cgccgactgc aagaaattgt gcccgagatt
    27061 gatattcagc aacgccacgc cgagcactgc acagttggtg gtgataagcg gcaaaaaaat
    27121 ccccagcaga cggtaaagca ccgggctggt tttgcgcacc accatctcgg tgaactgcac
    27181 gaccacagca atcaccagaa taaatgccag ggtgcgcagg taaatcagat taagtgggat
    27241 caaaatccac gtatcgataa gccaggcgca aatagacgcc agcgtcatca caaacgttgt
    27301 tgccagcccc atgcccatcg cggtttccag ttttttggaa acccccataa acggacagag
    27361 accgagaaac ttgaccagca caaagttatt gaccagtata gttccgacaa acagtaacag
    27421 gtagtcagtc atttttcggc ctgaaataaa aaagccgcct attatccgtt aatccagagc
    27481 aggcgacaac aggttaactg taaggttatt acggattcac aaacgtcgct tttacccgcg
    27541 acgagcgttt gaaataggag acgatcaatg cggttgccaa caaagtaaac agcaactggc
    27601 gaacggctat gccatcttcc acgggtgaaa aagcgaatgc ttttactgcc agcaatacgg
    27661 aaataagcag ccagataata tagtgtttag gaacacaacg ccgacgttta aagaaagcga
    27721 tggtcaacca cagtgtgtaa taccacatgg cgatagcggt aataaaagaa acgccccata
    27781 agatttgtgt cgtcagtgct tgtccgccga gcgtttgaaa tgtttgcgga gaagataacg
    27841 cagcggtata cagcaacaac gccagcgtcg tactcaacag agcgactaaa agccaggcca
    27901 acgggccaag caaccagcct ccaatacgtt gtggcgttgt ggtggtcata ggttctccag
    27961 aatagcgacg caaacaggca aatctaattt taggggcgtt attataaggc ttttataacc
    28021 gatcgctact ttctttattg gacatagtgc cagacggact tcggtacctg gccgaggtca
    28081 aacaaacgcc cgccagaaac cagctctgcg cgacggtgat cggcagcacg atacatattg
    28141 atcagttcct gatcgtccgt cagggtgtaa ttaagatggt cgtagagttt ttccagactt
    28201 tcgagtgaac tgattttgcg aaattttaat aagtagtcct gaacagtcat aaaaggtcca
    28261 taaaataatt aataccaaag gggtaggtat tattagtgat gataaaattt caccaaaagt
    28321 gaacgcttgt gttattacaa taataagaat gcgctgatgg caaatattca gggatgaaaa
    28381 actgctctga aaagagtaac gcgctttact atccctgaca atcaccaaca acatcgaaca
    28441 agataataaa ttcctggttt aatatccgac aagtgaaaac atgcaaccgg acgggcagca
    28501 tggcgctcta caagcgcaga gcttacttgt gttgtaccga agcactctgt tcaggtggct
    28561 gatagttgtc aatgtgactc gccacgccaa gaagaatgac tgaaacgaca agaacgatcc
    28621 aacctgttaa ttcaataaga cgattcatta cagcccacat ctctcttgat tgatccatta
    28681 acttcagggg gtaaatgtta cttagcaata atagctccag caagattttt actgaggttt
    28741 ttgcgatatt tagcttttgt cgttggaaaa ttcgctattt ttttgactta atattaaact
    28801 gttgagcgtg ggtaaaggat agtgtcaaat agcaatcata cttcaatgag aggcaatgac
    28861 atgagcgaca acatccgtgt tgggttgatt gggtatggtt atgcgagcaa aaccttccat
    28921 gcgcccctga ttgcgggcac gcccgggctg gaactggcgg taatctccag cagtgatgaa
    28981 acaaaagtaa aagccgactg gccaacggtt acggttgtct ctgagccgaa gcatctgttt
    29041 aacgatccca acatagacct gattgtcatt cctacaccca acgataccca tttcccgtta
    29101 gccaaagcgg cgcttgaggc gggtaaacat gtggtcgttg ataaaccctt taccgtgaca
    29161 ctgtcacaag cgcgagagct ggatgcgctg gcaaaaagcc tggggcgtgt gctgtctgta
    29221 ttccataacc gtcgttggga tagcgatttc ttgacgctaa aaggtttgct cgcggaaggc
    29281 gtgctgggtg aagttgctta ctttgagtct cattttgacc gcttccgtcc gcaggtgcgc
    29341 gatcgttggc gtgaacaggg cggcccaggc agcggtatct ggtacgattt agcaccgcat
    29401 cttcttgatc aggccattac gctatttggt ttaccggtca gcatgacggt agatttggca
    29461 cagttacggc ccggagcgca gtcgaccgat tatttccacg ccatcttgtc ctatccacag
    29521 cggcgagtca ttttacacgg taccatgctg gcagccgctg agtcagcacg gtatatcgtg
    29581 catggatccc gaggcagtta tgtgaaatat ggcctcgatc cacaggaaga acgtctgaaa
    29641 aatggcgagc gtctgccgca ggaagactgg ggctacgata tgcgtgatgg cgtacttacc
    29701 cgagtggaag gtgaggaacg tgtcgaagaa acgctgttga cggtacctgg gaattatccg
    29761 gcttactatg cggctattcg tgatgcgtta aatggcgatg gtgaaaatcc ggttccggca
    29821 agccaggcaa tccaggtaat ggagttgatt gagctgggca tcgaatccgc caaacatcgc
    29881 gcgactttgt gccttgcatg aaaaatggat ggtgcaattt gcaccatcca ttttgttact
    29941 tcgcggcgac tttttctcgc agtgcgcgtt tttcctcagc gctgaggaaa gccatttcca
    30001 gaccattaat ctgtgcctgg cggatttgct cgcgggataa cccagcagct ggcgcggcaa
    30061 cggtatattc gtgaatgata tccactccct gtacgccggg atcgtcagtg ttaatgccgg
    30121 cacgaatgcc atgctcaagg aacgttttca gcggatgtgc agccagctct gctacggtgc
    30181 tggtctgaat attggaggtc agacaggatt caataccaat ttgttgctcg gcgagaaaat
    30241 ccatcagcgc ccgatcttca atggctttta cgccatgtcc aatacgctcc gcacccagtt
    30301 cacgaatcgc ctgccagatg ctttccggcc cggcagcttc gcctgcatgg acggtaatat
    30361 gccagcccgc atcacgcgca aggttgaagt gagaaaggaa cagacttccc gggaaaccaa
    30421 gttcatcacc ggctaaatca agggcggtaa tctggtcacg gtgggctaaa aaggcctcca
    30481 gctcttgctg acaggcggct tcgccgaagg ttcggctcat aatgccgata agcttcgcct
    30541 gcacaccaaa agtgcggcaa ccttcacgta cgccatcgat caccgcttcg acaacacccg
    30601 ctacaggcag cttatgtgcc attgccatgt agcctggtga aaaacgcagc tcgacatagt
    30661 gcaggccgtt acgggctgca tcttcaatgt tttcaaatgc cacgcggcgg caggcatcaa
    30721 gagaggcgag aactttaacg ccccagtcaa gtttggtcag aaagctcacc agatcgggtt
    30781 cgttggcaat gacctgaacg tggggaatca gtgtttccag ggattgtgca ggaagcgaga
    30841 tattatactg gcggccaagt tcaagaatgg tctggggacg aatgttgcca tcaaggtggc
    30901 gatggatatc agttaatggc agggtggtat caatcatggt cgcactcttt ttctggatat
    30961 aaaagtgcgc accattataa aaacaaagca gggtaaaaat ccacgattgc gcaacgattt
    31021 ttacgttgcg caattggggt taacgaacag cgcggatggc gttaattaat ccagccacac
    31081 ctttttccag tttcgaacgt gggcagccgg cattgagacg gacaaaacca cgaccttctt
    31141 caccgtaggt ataccccggc atgatcgcga ctttttcttg ttcgataagt gctttttgca
    31201 acgcgttgtc gtcaatattc aacggacgta aatcaagcca tgccagataa gtggattgtg
    31261 ggatctgcca gttgagttca ggaaacgcgg cgttcatttt atctgcgata tacgtcaggt
    31321 tatctttcag atagatgcgt aaggcatcca gccacggcgc gccttgctga taggcggcga
    31381 tatgggcagt taacgccagt accgaagggg aagaaagccc atcacggcct ttcagtgccg
    31441 ataaataggc atcgcggcta ctgctatttt ctataatccc gtaagcaccg gtcagggcgg
    31501 gaatattgaa acttttcgag cccgacgtta gcaacgccca gtctccgcga gccacattac
    31561 tccagggaat atgcggctgc tcgccccaaa ccatatccat atggatttca tcggaaataa
    31621 cccgcacacc atgacgctcg cacaggtcag ccatgatctc cagctcatcg cacgtccaca
    31681 ctttcccggt aggattctgt gggctacaca ggagcataat tttacattct ggtttcgcca
    31741 acacggcttc caacttgccc atattgcaaa accaaccatc agcctgcttc tctaaagcaa
    31801 cgggcattac tgtgcgctgg ttaccttcaa tggccttgta aaatgcgtca taggcgggtg
    31861 tgtggatcac cacgccttca cctgtttcag accactgacg aatcagttct gaaaccatat
    31921 agatgacaga agggccatac accaccgtct gagaatcgat ggcggtgtaa tgctgggtgg
    31981 aaaaccagtg ggcaatagca gcgagaaact catcgttttt ccagcggctg tagccaaata
    32041 cgccgtgcat caggcgctga ttcagcgcct ctataatgca gggggcagtg gcaaaatcca
    32101 tgtctgaaat cgtgaacggt aacaggtcag cagtgccgaa acggtcagcg acataatccc
    32161 actgtgtaca ccatgtgcca tgacgatcca cgacctttga aaaatcgaac atatcttatc
    32221 cttatgcctg gacagtatgc atcagaccgg ccatttcatc ttttactgac tgaacttgtg
    32281 gcccgataac aacctgcagg ttatgttgat taagttgtac tacgccaatt gcccgattgt
    32341 ccttcagtgc ctgcacatta acaagcgaca tatctttcac agacaaacgc agacgggtaa
    32401 tgcagttatc gaggctgaca atattgtcgg caccgcctaa tgcttcgagg attgcaggaa
    32461 cgttgtaacc tgatttaccc ggcgcagcgg caacggcttt ttcgattgag ctggcaactt
    32521 cgctatcgcg ccccggggtt ttgagattga agcgggtgat agcgaaacgg aagatggcgt
    32581 agtaaacgac aaaccagatt gccgccacca ctggcaccat gtaccacttg gttgacagac
    32641 catgcaaaat accgaacacc acgaagtcga tgatattgcc gtcggtatta ccgatggtga
    32701 cgccgagcac agacatgacg gtgaagccga ggccggttaa cagcgcgtgg atgacataca
    32761 gaactggcgc tacgaacagg aacaggaatt ccagcggttc ggtagtgccg ccaacgacgc
    32821 aggcgatcag gccagaaatc agcagacctt taattttatg gcgattttcc gggcgcgcgc
    32881 agtgatacat agctaacgct gcagctggca gaccgccgag aaacgcaggc attttacctt
    32941 gcgaaaggaa acgcgtggcg ctttcagaaa aaccgtgagt ggtcgggcaa ctcaattgcg
    33001 tctggaagat ggtcagtgcg ccgctgacgg tttgaccgca gacttcctgc gtgccgcctg
    33061 cgtcggtaaa gcgaattaat gccaccagaa tgtgatgcag accaaacggc aacagcagac
    33121 gttcaccggt accaaacagc atcggtccga aatcacccgc gctgtttatc atatggccca
    33181 agccgctaat acccatggcg aaaatcggcc agactaatgg aatcaccagg ccgacaaggc
    33241 ccatcaccag cgaggagata attggtacga agcgcgtacc gccgaagaat gccagcgcat
    33301 ccggcaggcg gatattatgg aaacgctcat gcagcatcca gacgataata ccggcgatca
    33361 ccgcaccgag gatcccggta tcgatcgact ggatcccaag aatgctctgg atgttattgg
    33421 ctttcagaac cgcggcatcc gtggttggca gaatgccttt attggtcaac cagaagttta
    33481 ccgcgaggtt cattaccgca taaccgacga agccagcgaa tgccgctacg cctttatttt
    33541 cgcgtgccag gcccagcggg atggcgatac agaacatgac aggcaggaaa ctaaaagcaa
    33601 acgagccaat cttactcatc caggtaaaga tagcctgcaa cacggggttg cccaggaccg
    33661 ggatcagggt tatgacatca tggctgctaa gagaactacc aatgccgagc ataatgccgc
    33721 agaacgacaa taatgccacg ggtaacatga aggttttgcc taactgctgg aagaactccc
    33781 acagcgtgac tttcggtgct gttttcgccg tcataaaacg actcctcgta gagaacaatc
    33841 tggattcagt aaattgcgcg agaagataaa acgttttatc aaattttagt gaggcataaa
    33901 tcacattacg caacgataat agcgggtata agataaataa aaggtaaaac gttttatctg
    33961 tcacataatc agggagtagg tcatctgcat ggctaccgcc aaaaaaataa ccattcatga
    34021 tgttgcgctg gctgcgggcg tgtcggtaag taccgtttcg ctggtgctta gtggcaaagg
    34081 gcgaatctct accgccacag gagaacgcgt taacgccgcc attgaagagc tgggatttgt
    34141 gcgcaatcgc caggcgtcgg cgctgcgcgg cgggcaaagc ggcgtcattg gtttgatcgt
    34201 ccgtgattta tctgcgccgt tttacgccga attgacggcc ggattgacgg aagctctgga
    34261 agcgcaggga cggatggttt ttttgcttca cggcggtaaa gacggcgagc agctggcaca
    34321 gcggttttca ctgttactga atcagggggt cgatggtgtg gtaattgccg gggctgcagg
    34381 aagcagcgat gacctgcgac ggatggcaga agaaaaagct atcccggtga ttttcgcttc
    34441 ccgtgccagt tatcttgatg atgttgatac ggttcgcccg gacaacatgc aggctgcaca
    34501 gttgttgacg gagcatctca ttcgcaatgg gcatcagcgg atcgcctggc tgggagggca
    34561 aagttcctca ttaacccggg cagaacgggt gggaggctat tgtgcaactc tactaaaatt
    34621 tggtctgccg tttcacagcg attgggtgct ggagtgcaca tccagccaga agcaagctgc
    34681 ggaagctatc acggcgcttt tacgtcataa cccgaccatc agtgctgtgg tttgctataa
    34741 cgaaactatt gcgatggggg catggtttgg tttgctgaaa gcagggcggc aaagcgggga
    34801 aagcggagtc gatcgttact ttgagcaaca agtttcgctg gcggcattta ccgatgcgac
    34861 accaaccaca cttgatgata tacccgttac ctgggccagc acgcctgcgc gggaacttgg
    34921 taccacactt gcggatcgca tgatgcaaaa aatcacccat gaagagacgc attcacgcaa
    34981 tcttattatt cccgcccggc tcattgcagc gaaataatcc tctctttatc tgctatacct
    35041 ggtagtgtcc tttcctcaag gttaatgata aaaaatgtgg cacacagg
//
LOCUS       DAZPEB010000019        34471 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZPEB010000019 DAZPEB010000000
VERSION     DAZPEB010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34471
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.019"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            40..894
                     /gene="ynfH"
                     /locus_tag="ACHURJ_000845"
     CDS             40..894
                     /gene="ynfH"
                     /locus_tag="ACHURJ_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFR1279901.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCASLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            937..1551
                     /gene="dmsD"
                     /locus_tag="ACHURJ_000846"
     CDS             937..1551
                     /gene="dmsD"
                     /locus_tag="ACHURJ_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFR1279902.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQYEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELSRLTLAQWQSQLLIPVAVKPLFR"
     gene            1710..3002
                     /gene="clcB"
                     /locus_tag="ACHURJ_000847"
     CDS             1710..3002
                     /gene="clcB"
                     /locus_tag="ACHURJ_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFR1279903.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTEMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(2955..3650)
                     /gene="bioD"
                     /locus_tag="ACHURJ_000848"
     CDS             complement(2955..3650)
                     /gene="bioD"
                     /locus_tag="ACHURJ_000848"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFR1279904.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(3775..4995)
                     /gene="mlc"
                     /locus_tag="ACHURJ_000849"
                     /gene_synonym="dgsA"
     CDS             complement(3775..4995)
                     /gene="mlc"
                     /locus_tag="ACHURJ_000849"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFR1279905.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNLGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPTYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLHG"
     gene            complement(5130..6023)
                     /locus_tag="ACHURJ_000850"
     CDS             complement(5130..6023)
                     /locus_tag="ACHURJ_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1279906.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPGVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRAQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPASRNFRIHLLNALR"
     gene            6130..7382
                     /locus_tag="ACHURJ_000851"
                     /pseudo
     CDS             6130..7382
                     /locus_tag="ACHURJ_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(7431..7550)
                     /locus_tag="ACHURJ_000852"
     CDS             complement(7431..7550)
                     /locus_tag="ACHURJ_000852"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFR1279907.1"
                     /translation="MTHSKMNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            7806..8093
                     /gene="asr"
                     /locus_tag="ACHURJ_000853"
     CDS             7806..8093
                     /gene="asr"
                     /locus_tag="ACHURJ_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFR1279908.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTTKAAPAKTTHHKKQHKA
                     APAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQPAA"
     gene            8186..8269
                     /locus_tag="ACHURJ_000854"
     CDS             8186..8269
                     /locus_tag="ACHURJ_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1279909.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            8369..9190
                     /locus_tag="ACHURJ_000855"
     CDS             8369..9190
                     /locus_tag="ACHURJ_000855"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFR1279910.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTAQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVVLRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTNDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(9229..9558)
                     /gene="mdtI"
                     /locus_tag="ACHURJ_000856"
     CDS             complement(9229..9558)
                     /gene="mdtI"
                     /locus_tag="ACHURJ_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFR1279911.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(9545..9910)
                     /gene="mdtJ"
                     /locus_tag="ACHURJ_000857"
     CDS             complement(9545..9910)
                     /gene="mdtJ"
                     /locus_tag="ACHURJ_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFR1279912.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            10322..11356
                     /gene="tqsA"
                     /locus_tag="ACHURJ_000858"
     CDS             10322..11356
                     /gene="tqsA"
                     /locus_tag="ACHURJ_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFR1279913.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMLSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(11381..12769)
                     /gene="pntB"
                     /locus_tag="ACHURJ_000859"
     CDS             complement(11381..12769)
                     /gene="pntB"
                     /locus_tag="ACHURJ_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFR1279914.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQDVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(12780..14312)
                     /gene="pntA"
                     /locus_tag="ACHURJ_000860"
     CDS             complement(12780..14312)
                     /gene="pntA"
                     /locus_tag="ACHURJ_000860"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFR1279915.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     TGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRRVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            14835..15779
                     /gene="ydgH"
                     /locus_tag="ACHURJ_000861"
     CDS             14835..15779
                     /gene="ydgH"
                     /locus_tag="ACHURJ_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFR1279916.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            15965..17347
                     /locus_tag="ACHURJ_000862"
     CDS             15965..17347
                     /locus_tag="ACHURJ_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR1279917.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLVLGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAASLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            17384..18106
                     /gene="folM"
                     /locus_tag="ACHURJ_000863"
     CDS             17384..18106
                     /gene="folM"
                     /locus_tag="ACHURJ_000863"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFR1279918.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKLGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(18103..18426)
                     /locus_tag="ACHURJ_000864"
     CDS             complement(18103..18426)
                     /locus_tag="ACHURJ_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFR1279919.1"
                     /translation="MKAALGVLVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHYIVAS
                     ERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWVLIIC
                     WIKLH"
     gene            18552..19272
                     /gene="rstA"
                     /locus_tag="ACHURJ_000865"
                     /pseudo
     CDS             18552..19272
                     /gene="rstA"
                     /locus_tag="ACHURJ_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
     gene            19276..20577
                     /gene="rstB"
                     /locus_tag="ACHURJ_000866"
     CDS             19276..20577
                     /gene="rstB"
                     /locus_tag="ACHURJ_000866"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFR1279920.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLYERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLVQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            20653..21582
                     /gene="tus"
                     /locus_tag="ACHURJ_000867"
     CDS             20653..21582
                     /gene="tus"
                     /locus_tag="ACHURJ_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFR1279921.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRSLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(21579..22982)
                     /gene="fumC"
                     /locus_tag="ACHURJ_000868"
     CDS             complement(21579..22982)
                     /gene="fumC"
                     /locus_tag="ACHURJ_000868"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFR1279922.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(23125..24771)
                     /gene="fumB"
                     /locus_tag="ACHURJ_000869"
     CDS             complement(23125..24771)
                     /gene="fumB"
                     /locus_tag="ACHURJ_000869"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFR1279923.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            24970..26145
                     /gene="manA"
                     /locus_tag="ACHURJ_000870"
     CDS             24970..26145
                     /gene="manA"
                     /locus_tag="ACHURJ_000870"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFR1279924.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            26246..27754
                     /locus_tag="ACHURJ_000871"
     CDS             26246..27754
                     /locus_tag="ACHURJ_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFR1279925.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITVAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(27980..29245)
                     /gene="uidC"
                     /locus_tag="ACHURJ_000872"
     CDS             complement(27980..29245)
                     /gene="uidC"
                     /locus_tag="ACHURJ_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFR1279926.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQRGLFDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPCENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGTGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(29284..30657)
                     /gene="uidB"
                     /locus_tag="ACHURJ_000873"
     CDS             complement(29284..30657)
                     /gene="uidB"
                     /locus_tag="ACHURJ_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFR1279927.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVACVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYERYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARSGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            complement(30654..32454)
                     /gene="uidA"
                     /locus_tag="ACHURJ_000874"
                     /pseudo
     CDS             complement(30654..32454)
                     /gene="uidA"
                     /locus_tag="ACHURJ_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
     gene            complement(32842..33432)
                     /gene="uidR"
                     /locus_tag="ACHURJ_000875"
     CDS             complement(32842..33432)
                     /gene="uidR"
                     /locus_tag="ACHURJ_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFR1279928.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(33661..34428)
                     /gene="hdhA"
                     /locus_tag="ACHURJ_000876"
     CDS             complement(33661..34428)
                     /gene="hdhA"
                     /locus_tag="ACHURJ_000876"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFR1279929.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
BASE COUNT         8418 a         8904 c         8369 g         8780 t
ORIGIN      
        1 ataccactgg ttatctggct aatccggagg aagtgtaaaa tgggaaatgg atggcatgaa
       61 tggccactgg ttatctttac cgtattgggt caatgcgtag taggcgcgct gattgttagc
      121 ggaattggct ggttcgccgc caaaaatgac gcagaccgtc agcgtattgt ccgtggcatg
      181 tttttcctct ggctgttgat gggcatcggg tttattgcct ctgtcatgca cctgggatcg
      241 ccactgcgag cgtttaactc gctgaatcga atcggagctt ctggtctgag taacgagatt
      301 gctgccggtt ccatattttt tgccgttggt ggactatggt ggctggtggc ggtcatcggt
      361 aaaatgccgc aagcgttggg gaaactctgg ttacttgtca gcatggcgct cggcgtcatt
      421 ttcgtctgga tgatgacctg cgtgtatcaa atcgacaccg tgccaacctg gcataacggt
      481 tacactacgc tggctttctt cctgacggta ttgttgagcg gtccaatact tgctgccgca
      541 attctgcggg cagctcgcgt tacatttaat accacaccat ttgccatcat tagcgttctg
      601 gcgctgatcg cttgtgccgg ggtgattgtg ctgcaaggtt tgtctctggc gtctattcac
      661 tcatccgtgc aacaagccag cgcactggta ccagattacg cctccttaca ggtctggcgt
      721 gtggtattgt tatgcgcaag tcttggatgc tggctttgcc cgctcattcg tcgccgtgaa
      781 cctcacgttg ccggacttat tctggggcta atcctcatac ttggcggtga gatgatcggg
      841 cgtgtgctct tttatggcct gcacatgaca gtaggaatgg ctattgcagg ttaacaaatt
      901 ggtgcggggc aacccgcaca tttcaggatg ttaggaatga cccatttttc acagcaagat
      961 aatttttctg tcgcggcgcg cgtgttgggc gcgctgtttt attacgctcc agagagtgcg
     1021 gaagccgcac ctctggttgc ggtactcacc agtgacggct gggaaactca gtggcctcta
     1081 ccagaagcgt cattagcgcc gctggtgacg gcttttcaga cacagtgtga agagactcac
     1141 gcccaggcct ggcagcgttt gtttgtcggc ccgtgggcac tgccgtctcc gccgtggggg
     1201 tcggtctggc tggatcgcga atctgtgctg tttggcgatt caacattggc acttcgtcag
     1261 tggatgcgcg agaaaggcat tcagtttgaa atgaagcaat acgaaccgga agatcatttt
     1321 ggttcactgc tgttgatggc tgcgtggttg gcggagaatg gtcgccagac agaatgcgaa
     1381 gaattgctgg catggcacct ttttccgtgg tcaacacgtt ttcttgatgt ttttatcgaa
     1441 aaagcagaac accctttcta ccgtgcactg ggtgaactgt ctcgcttaac gctggcgcaa
     1501 tggcaatcac aactgttaat tcctgtcgcg gttaaaccgc tgtttcgata ggatcaccgt
     1561 aatattgccg gatggtgacg attacgcgca atccggcaat aataggttac agtgtcacgt
     1621 ttttttatct cttaaagcac gcactgcttt tgcggctggc ctcttttgcc gcaaaatagt
     1681 cgcccgtgtt tcattgccca tttctgctca tgcatcatct acacatctat ccggatctgc
     1741 gcactatgtt ccgccgtctg cttatcgcaa cagtcgtcgg tattctcgcg gcctttgccg
     1801 ttgccgggtt tcgtcatgcg atgctgctac tggagtggtt gttcctcaat aatgactccg
     1861 gcagtctggt caatgcagcg acaaaccttt ccccctggcg acggttgcta actccggcgc
     1921 tcggcggact ggcggcgggt ttgttgctga tgggctggca gaaatttacc caacaacgcc
     1981 ctcatgcgcc gaccgattac atggaagcgt tgcaaaccga tggacagttc gattacgcag
     2041 caagcctggt taaatcgctt gcctcgctgc tggtagtaac cagcggcagt gcaattggtc
     2101 gcgaaggtgc gatgattctt ttagctgccc ttgccgcctc ctgttttgcc caacgtttta
     2161 cgccacgcca ggagtggaaa ttatggatcg cctgtggcgc cgcggcggga atggctgcgg
     2221 cctatcgtgc cccgcttgct ggcagtttat ttatagccga agtgctgttt ggcactatga
     2281 tgttggcctc tctcggcccg gtgattattt ccgccgtcgt ggcattgctg gttagcaatc
     2341 tgattaatca tagcgacgcg ttactctaca acgtacaact ctcagtgacg gttcaggctc
     2401 gtgactatgc gctgattatc agtacaggtg tgctggcagg tctgtgcgga ccactgttgt
     2461 taacgttaat gaacgcctgt catcgtggat ttgtgagtct caaacttgcg ccgccctggc
     2521 aactggcact aggcgggttg atcgtgggtc tgctttccct gttcacacct gcagtgtggg
     2581 gcaacggcta tagcaccgta caatcctttt taaccgcccc accactgtta atgatcattg
     2641 ccgggatctt cctttgtaaa ctgtgtgccg tgctggcgag tagcggttcc ggcgcacccg
     2701 gtggtgtctt tacaccgacg ctatttatcg gtcttgccat tggcatgttg tatggtcgta
     2761 gcctgggatt atggttccct gatggcgaag aaattacgct tttactcgga ttgaccgaga
     2821 tggcgacact gttggcggca accacgcacg cgccgattat gtcgacgttg atgatatgtg
     2881 aaatgaccgg ggagtatcag ctactccccg gtttattgat tgcctgcgtc attgcgtcgg
     2941 taatttcgcg gacgttacac cgtgactcta tctaccgcca gcacactgcg cagcatagct
     3001 aagcggatgt attgccccag ttctcgctgt tcggcgcgcg gcagataagg cagttcacca
     3061 atgagcggtg ccggaagttt tttacccagc acatcaatga tttccgcata atgcgccagt
     3121 cctgggttga ttcggttagc cacccagcca atgagcggca gcccgtcgtt ggcaatcgcc
     3181 tgagctgtta gcagtgcatg gttaatgcaa ccttcctgaa taccgacaac catcaacacc
     3241 ggcaactgtt cctgcaccac ccattcagag agtggacgca aatcattcat cagactgcgc
     3301 cagccgccag tcccttctac cacgacatga tcgactttat cggtcaggtt tgccaggccg
     3361 tttgaaatga gggtgtaatt gattgggcaa ctgtgcgcca cgctactttc ttcttcgctt
     3421 aacgcgatag gattaactgc ttcataaggc agttcgatgg ttgaaacact ctgcaacacc
     3481 agggcatctt tattacgcag cccttcgggt gtctctttgc tccccttcgc tacgggttta
     3541 tatcccgcaa ctgtttttcc ctgggaggct aacgcttgta gcaatgcgcg ggaaaccacc
     3601 gttttcccta cagaagtgtc tgtaccggta ataaagaaac gcttcagcat cactaactcc
     3661 accgttatgc ttcacaaata taaactagga aaataattaa ccttgaaagt ctaagttatg
     3721 ctttcctggc ccaaattgag atagcgcaaa ttttggtaga acagttaaaa aatgttaacc
     3781 atgcaacaga cgaatcaaca aagaaccgtt atacatcgcg tcttttacca gcgcagcgcc
     3841 tgccatcgtg ccctggttag aaaactgagt actctcaacg ctaatgtgct gactatacgt
     3901 aggaagggcc tgctgacgta tgctgtccga gatgacaggg aagaggatat ctgccgcttt
     3961 acttaacggt gagccaatca gtattttttg tgggttaaat aaattcacca tgatggcaag
     4021 aatgcgcccg acatgcgcgc ccaccccggt aatgatgtct tttgccagta gatcgccgcg
     4081 caatgccgcc tgacacaatg agtccacggt taacggttgc ccatgtaaca tcgagctcat
     4141 ggattgattg agacgcagct gtgccagctc aagaatactg tccacactgg cgatggtttc
     4201 gaggcagccg aggttcccgc aataacagcg tttaccatat gggtcgacct gtgtgtggcc
     4261 tatttccacg agactactgc tgcctgcgtg tagcagatga ccatcggtaa tgacgcccgc
     4321 ccccacgttg tgatcgataa ccacctgaat cacatcgcgc gccccgcgtg aggcaccaaa
     4381 caaggcctct gccatcgtcc atgcgctgat atcatgctga atataaaccg gtacgccggt
     4441 atgctgctcc agcgcctcgc cgagcggcat ctcttttaca tcctcgtaga acggcatgcg
     4501 atgtacaata ccattttccg tatcaataat tcccggcagg gttatggcaa tcgaagttag
     4561 acgctcaagt tttttctggt ggcggataaa aaactgatcg atatgggaaa tgatacgatc
     4621 cagcaatggc aagtcatctt ttaacgccag ttcctgcgac tcttccacca ccagtttgct
     4681 gctcagatcg cgcagagcaa ggaaaatctc accgcgacta atgcgcagag aaagatagtg
     4741 ccaggcttca gtttcaacca ccagtcccac cgccggacgg ccacggttcc ccgcttcttt
     4801 gatttccagc tcttgcacca agtgtgcttc gagcatctca cggacaattt tagtgatact
     4861 ggcaggagcc agctgcgcca gacgggaaag atcgatacgc gagactggac caagctgatc
     4921 aatcaggcga taaaccgcgc ccgcgttggt ctgctttatt tgatcaatgt gcccaggctg
     4981 gttttcagca accaccgcat actccctata ttttcgcgct ccgaaataat ctgtaggcta
     5041 tggtgaagca cttcaatacg tgtcgtcaaa tttttactta gacatgtgat taacagcaca
     5101 tttttcgggc tttttcgctg aaatttccct cacctgagag cattcagcag atgaatacga
     5161 aagttacgcg aagctggact ttgttcatga tgtttcggcc agaccaacca catttcagaa
     5221 accgcatcct cttcagcaat cggcacccag cgcatttcgt tgagctgagc acgtttaaat
     5281 gaagcaggca aaattgaaac acccagaccg gcggaaacca gaccgatgat ggtcattgcc
     5341 tcgcccacct cctgagtgat gacgggcgtc aaatggtaac gtcgcatcag cccgagaata
     5401 tcgtcataca gccctgtccc gacgtgcgga tcaaaaaaga cgaagggttc tttcgccagt
     5461 tcagccaacg ttacattcgg gttatttgcc aggggatgat cgtgcgggat catcgccata
     5521 agcggttcat ggacgatgac tgcgtgttca agcgtctccg gtaacgctgt gttacgcagc
     5581 aatcccatat ccagcgttcc ttcaatgagc ggagcgattt gctcacgagt gttcatttcg
     5641 cgggtttgta aatggacacc gggataatca cggcgaaaca gcgataacgt atcggacacc
     5701 gcccgaataa aaggagcgga cgaagtaaaa ccaatgcgca actcccccgc ttcaccctga
     5761 tgcagtcttt cagcgcgagc ggcagcgtca tctaccatag acaggatttg ccgactatct
     5821 gcaagaaact gttttcctgc tgccgtcagc aatacactgc gattagttcg tgccagcagt
     5881 cgggcaccaa tttgttgctc cagtgcctga atctgctgac ttagcggtgg ttgcgaaata
     5941 ttcaggcggg cagcggcgcg cccgaaatgc agctcttccg caacagcaac aaagtaacgc
     6001 agatgacgaa gttcaatatt catatttaaa acatcttatt tgagattatt aatatattag
     6061 acagaacaat tcgattttcc taacctatgt ataagcctga tccacaggca tatttagcaa
     6121 ggatttcaag tgagccgtac tacaactgtt gatggcgctc cggcaagcga cactgacaag
     6181 caaagcattt ctcagccaaa tcaatttatt aaacgcggta cgccgcaatt tatgcgcgtc
     6241 accctggcgc tgttctctgc cggactggca acctttgcac tcctctattg tgtgcagcct
     6301 atccttccgg tgctttcgca ggagtttggc ttaacccccg cgaacagtag tatttcactg
     6361 tccatttcca cggcgatgtt ggctattggt ttgctgttta ctggccccct atccgatgcc
     6421 attggtcgca aaccagtgat ggtcacggcg ctactgttgg cctccatttg tacgttattt
     6481 cgacaatgat gaccagctgg cacggcattt tgattatgcg cgccttgatt gggctttcgt
     6541 taagtggcgt ggcagctgtt ggcatgactt atcttagcga ggaaatccac cccagtttcg
     6601 tggccttttc gatggggttg tatatcagcg gcaactcaat tggcggcatg agcggacgct
     6661 taattagtgg tgtcttcacg gactttttca actggcgaat tgctctggcg gcaatcggtt
     6721 gtttcgcgct agcctcggcg ttgatgttct ggaaaatcct tccggaatca cgccattttc
     6781 gcccgacttc gctgcgacct aagacgttgt ttatcaactt tcgtctgcac tggcgtgacc
     6841 ggggattacc gttattgttc gcagaaggct ttttgctgat ggggtcgttc gtcacgctgt
     6901 ttaattacat cggctatcgg ttgatgcttt ccccctagca tgtcagtcag gccgtggttg
     6961 gcttattatc gctggcttat ttgaccggta cacggagctc acccaaagcc ggaaccatga
     7021 ccacccgcta cgggcgcggt ccggtgatgt tgttttcgac gggggttatg ctgtttggtt
     7081 tactgatgac cttattcagc tcgctgtggc tgatctttgc cggaatgtta ctcttctcag
     7141 caggattctt cgcagcccac tcagttgcca gcagctggat cggcccccgc gcaaaacgcg
     7201 ctaaaggcca ggcctcctcg ctgtatctgt tcagttacta tctggggtcg agtattgccg
     7261 ggacgctggg tgatgttttc tggcataact atggctggaa cggcgtcggc gcatttattg
     7321 ctctgatgct ggtcattgct ctgctggtcg ggacgcgttt gcatcgtcgt ctgcacgcct
     7381 gaaaaataag cccggactgc ggtaactaca cgtccggact tattgcttgc tcaaaccaac
     7441 gttaatagcc atcctaaaat agacgaagcg ccagccaatt cccgcagtgc gtctagcgtc
     7501 atcaggatta taagtaccca aataaacgga ttcattttgc tgtgtgtcat ttattactga
     7561 tgcgcagtta ttctaccgct ttgtaagtag taaaatagtt aacccgatca agactactat
     7621 tattggtagc taaatttccc ttaagtcaca atacgttatt atcaatgctg taatttattc
     7681 agcgtttgta catatcgtta cacgctgaaa ccaaccactc acggaagtct gccattccca
     7741 gggatatagt tatttcaacg gccccgcagt ggggttaaat gaaaaaacaa attgagggta
     7801 tgacaatgaa aaaagtatta gctctggttg ttgccgctgc tatgggtctg tcttctgccg
     7861 cctttgctgc agagactgcg acgaccacca aagcagcgcc ggcgaaaacg acacatcata
     7921 aaaaacagca taaagcagca cctgcccaga aagcgcaggc agctaaaaag catcataaaa
     7981 atacgaaagc tgaacagaaa gcccctgaac aaaaagcgca ggcagcgaag aaacacgccg
     8041 ggaaacacag tcatcagcaa ccggcaaaac ctgctgcaca acccgcagcg taagttttca
     8101 acagtaatgc tggcgcgccc cctcgcgcct gaaaattacg gtgctaagcg ggtaacgttt
     8161 agcaccgcct ttagcggagg gtagtatggt gggccgttat cgctttgagt tcattcttat
     8221 catccttatt ttatgcgcac tgattgccgc ccgtttttat ctttcctgac tgtagttatc
     8281 tgattttact cccactttca tcccgtcccg tctatagtat ttatgagggt ttgcttttaa
     8341 taatcataat tacccaccag agtgtgatat gcgtacaacc attgctgtag tgttgggtgc
     8401 aattagtttg acgtctgctt ttgtgtttgc agataaacca gacgttgcca aatcggcaaa
     8461 cgatgaggtc agcaccctgt tttttggtca tgatgatcgt gtgccagtga atgacacggc
     8521 ccaatcaccg tgggacgcgg ttgggcaact ggaaacggcc agcggcaatt tatgtacggc
     8581 gacgctgatt gcacccaatc tggcattaac ggcaggacac tgtttattga cacctccaaa
     8641 gggtaaagcc gataaagcag tggtgctgcg ttttgtgtca aataaaggtc tttggcgcta
     8701 tgagatccac gacatagaag gccgcgttga tccgacactg ggaaagcggt taaaagcaga
     8761 tggggatggt tggattgtac ctcccgcagc cgcgccgtgg gacttcggat tgattgtgct
     8821 acgtaatccc ccttctggca ttacaccgtt gccgttattt gagggagata aagccgcgct
     8881 tactgccgca ttaaaatcgg ctggccgtaa agtaactcag gcgggatacc ctgaagatca
     8941 tctcgatacg ttgtacagtc atcaaaactg tgaagtgact ggctgggcgc aaacgtcggt
     9001 gatgtcacat cagtgcgata ccttgccggg tgacagcggt tcgcctctga tgttgcatac
     9061 caatgacggc tggcaattaa ttggggtgca aagttcggct cctgccgcga aagatcgctg
     9121 gcgcgccgat aaccgggcca tttctgttac cggttttcgc gacaagctgg atcaactgtc
     9181 gcaaaaataa tgttcaaacg ctgcccgact gtgcgggcag cgtgttcatc aggcaagttt
     9241 caccatgatc attccagcca acagcaagac caggccaatc cagcctttac gatttaaccg
     9301 ttgaccaaac aagatccaac ctgcggctaa cgtggcggca ataccaaacc cgccccacaa
     9361 tgcataagcg acagacaagt cgatcccttt aacggcctga gaaagcgcac taaaggcagc
     9421 cagcaccgcc gccagggaga gcaagccaaa tattttgcga cgaaagccgt cagaaaattt
     9481 caaaaagacg ttagcaacga tttccagcac gattgccaat gccagccagg cggcgtgaac
     9541 ccattcaaac tgcgccatgg tttacctcca gttcaggttt acgcgcttta cgggtacctg
     9601 attttatcaa cacaatcccg gcgaccaggg tggttaaccc ggcaattttc atcagcgata
     9661 aactttcgtc gaataacaaa acgctaaaca aggtaataaa taaaataccg ataccttccc
     9721 acagcgcata agctacgcct aaggcgatct ttttaacggc gaaagagaga aatatatacg
     9781 acagagaaat catcaccagc attaaaataa agccgccatt tccctcactg acgctcgccc
     9841 atttcattga cagcgtaccg gtaatttctg tagcaatagc caggcctaat aaaatccaat
     9901 aaatatacat tgtccttctc ctgcaagaga attattttaa ttttcgctta attcagcgaa
     9961 attaaagtaa agtggtcaag ccccgcgcat agggcaagct taggcagaag aaaggactaa
    10021 agcgcgttgc gccagtgctg ctcacctacg agcaagatag tggatgaggt acaaagatgg
    10081 gtaaatgttg aaaataaggt cctgaacaaa ttgtccataa tattacaatt atccgcagtg
    10141 ttgcttctcg tcatcgcgga tgataattgt cctcggtagt tgaacacgcc tgatttgtat
    10201 catagcttaa gaattaactc aaaatatttt cacttcttta cctgagcggt ttgattttcg
    10261 ttatgatgac ggagcgaaaa agacattatt attagcaaag gaagaaaaaa cggggacaag
    10321 catggcaaag ccgatcatca cgctcaatgg cctaaaaatc gtcattatgt tgggaatgct
    10381 ggtcattatt ctctgcggta tccgttttgc cgccgagatc atcgtgccgt ttattctcgc
    10441 attatttatt gctgttattc ttaacccgct ggtgcaacac atggtccgct ggcgagtgcc
    10501 gcgtgtactg gcggtgtcga ttttgatgac catcatcgtg atggcgatgg tgttgctatt
    10561 agcttatctg ggttccgcgc tcaacgagtt gacgcggacg ttaccgcaat atcgcaactc
    10621 tattatgacg ccgctgcaag ctcttgagcc gttgttgcaa cgcgtaggga ttgacgtctc
    10681 agttgaccag ctggcgcatt acattgatcc gaacgcagcg atgacgttgc tcaccaactt
    10741 attgacgcag ttatctaatg ccatgttatc aatattttta ttgctgctga cggtgctgtt
    10801 tatgctgctc gaagtgccac aattgcccgg aaaatttcag caaatgatgg cgcgtccggt
    10861 tgaagggatg gcggcgattc aacgtgcaat tgacagtgtt tctcattatc tggtgctgaa
    10921 aacagccatc agcatcatca ccggcctggt cgcctgggcg atgctcgccg cactcgatgt
    10981 tcgcttcgct tttgtctggg gattgctggc ctttgcgctt aattacatcc ctaatattgg
    11041 ttcagttctc gcggcaatcc cccctatcgc tcaggtactg gtgtttaatg gcttctacga
    11101 agcgttgctg gtgctggcgg gatatctgct gattaatctg gtcttcggca atattctgga
    11161 gccgcgcatc atgggacgtg ggctggggct ttccacattg gtggtatttt tgtcgttgat
    11221 tttttgggga tggttgttag gaccggtggg tatgctgctt tccgtgccgt taacaattat
    11281 tgtcaaaatt gcgcttgaac aaacagcggg aggtcaaagc atcgccgttc tgttaagcga
    11341 tctcaataaa gagtgacggc ctccgcagag gccgtcaggg ttacagagct ttcaggattg
    11401 catccacgct ggctttggcg tcaccaaaca gcatgtgggt gttttccttg aagaacagcg
    11461 ggttttgcac accagcatag ccagtgttca tcgaacgttt aaagacaatc acgtcctgcg
    11521 ctttccacac ttccagcaca ggcataccag caataggact cttcggatca tcctgcgccg
    11581 ccgggttaac cgtatcgtta gcaccaatca ccagtacggt atcggtatca gcaaagtcat
    11641 cgttgatctc gtccatttcc agcacgatgt catacggtac ttttgcttca gccagcaata
    11701 cgttcatatg tccaggcaaa cgccccgcaa ccgggtggat accgaaacgc acgttgatac
    11761 cacgagcgcg cagtttctcg gtaatttcag cgacaggata ttgcgcctgc gcgactgcca
    11821 tgccgtaccc cggagtaatg atcactgaat gggagttttt cagcagttcc gctgtctctt
    11881 ctgcggtgat ttcgcggtgc tcgcccactt cctgatcatc gccagtagaa gagccgtcgg
    11941 tgccgaaacc acccgcaata acgctgataa aggaacggtt catcgcctta cacataatgt
    12001 aagaaaggat agcccccgaa gaaccgacca gcgcaccggt cacaatcagc aggtcgttgc
    12061 tgagcataaa gcccgcagcc gcagccgccc agccggagta cgagttcagc atcgacacca
    12121 ccactggcat atctgcacca ccgatggagg cgactaaatg ccagccgaat accagcgcaa
    12181 tggcggtcat tattagcaat gccagcactt gcaggccgac gctgtccgtg cgaacaaata
    12241 caatcagcag caggaaggaa acgaccagag ccgccaggtt cattttgtga cggtttggca
    12301 gcatcaacgg tttagacgaa atcttgccac acagtttgcc gaacgccacc accgaacccg
    12361 tgaacgttac cgccccgatg aagataccga ggaacacttc cgtcaggtga atattgacca
    12421 gaatcggtgc cattcccgcg tcatgatgca gatagctgtt aaagccaacc agcactgccg
    12481 ccagacccac gaagctgtgc aggatcgcca ccagttctgg catttcggtc atttcaactt
    12541 tcttcgccag acggatacca attgccccac caatgaccat cgccagcaag atccagccaa
    12601 cattacccgt atccggtcca aaaatggttg cgattaacgc aatcgccatc cctgcgatac
    12661 cgaagttgtt accctggcga gacgtttcat gtttcgaaag accggccaga ctgaagataa
    12721 acaggatcgc ggcaacaatg tatgcagctg taactaatcc tccagacata tgttacccct
    12781 taatttttgc ggaacatttt cagcatgcgc tgagtcacgg tgaagccacc gaaaatatta
    12841 atgctggcta taagcaccgc gataaaacta aggaagctaa cccagccgcc ctggccaatc
    12901 tgcaacagtg ctccgacaac aataatccct gaaatcgcgt tggtgaccga catcaacggt
    12961 gtatgcagcg cgtgcgatac attccacacc acgtaataac cgacaacgca ggccagcgcg
    13021 aaaacggtga agtgcccaag aaactctttc ggcgcaacgc ttgccagcca accgaaaagg
    13081 atgattgcca gcgccatcaa cgcgtattta cgccacggtg agcaggcaca tttttcctca
    13141 gttttcactt ccggtgccgc tttttgtgcc gcctgcggct gagctgatac ctgaatcggc
    13201 ggtgccggcc aggtaatttc gcccgcacgg atcacggtca cgcggcgaat caccacatca
    13261 tcaaaatcaa cagtgatatt gccgtctttc tctttgcaca acagtttcag cagattaacg
    13321 aggttagtac cgtaaagctg cgaggattgc gtcggcagac ggcccggaag atcggtataa
    13381 ccaatcactt tgacaccatt ttccgtagtg aagatttcac ccggcacggt gtattcacag
    13441 ttgccaccgt tttgggctgc caggtcgaca atcacactgc ccgtcttcat ggagtcaacc
    13501 atttcacggg taattagctt cggcgctggt ttgcctggaa taagcgcggt ggtgacaatg
    13561 atatcgacct cttttgcctg ggcggcaaag agttccattt ctgctttaat gaacgcgtcc
    13621 gacatcactt tggcatagcc atcgccgctg cccgcttcct ctttaaaatc cagctcgagg
    13681 aattccgcgc ccatactttg aacttgttct ttcacttccg gacgggtgtc gaatgcacgc
    13741 acaatcgcgc cgagactgtt tgctgcgcca atggcggcca gacctgcaac acccgcacca
    13801 atcaccatca cttttgccgg tggcactttc ccggccgcag taatttgccc ggtaaagaag
    13861 cgcccaaatt catgtgccgc ttcaacaatg gcgcgataac cggcgatgtt cgccatcgag
    13921 cttagtgcgt ccagcgattg tgcgcgtgag atacgcggca cagaatccat cgccatcacg
    13981 gtcacgttac gttccgcaag tttttgcatt aattccggat tctgcgcagg ccagataaaa
    14041 ctcaccagcg ttgtcccagg attcagtaac gcaatttcat catctaacgg cgcattgacc
    14101 ttcagaatga tctctgactg ccagacgcta ttcccttcta caatttcagc gcccgcttgc
    14161 acaaacgctt tatcgtcaaa acttgccagt tgacccgcgc cgctctctac cgcgacggta
    14221 aaacccagtt tcagcagctg ttccactgtt tttggcgttg ctgcaacacg ggtttcattg
    14281 gttaaccgtt ctcttggtat gccaattcgc atgatattcc cttccatcgg ttttattgat
    14341 gatggtttgc ctgtgtcagg agccacacaa gccgctcatg tacgagctaa atgttactcc
    14401 gttaaaataa attagtaaca aacgccctat aacgtactga aaattatacc tgtgatctag
    14461 cgccaaaaat cagtatttcg gcgtgaactc gcaaaatatt aacgattcag ccgtgatagt
    14521 gggataaaca ccttagaacg ccggataaag actgataatt gtcttcgacg gtcgggtaaa
    14581 acgagacatc gccccggcac gattcactac ttaacattaa attaacttat acaattcagt
    14641 tgcttcagta gtaatgatgc tgatacggct gttttttaag catagacggt catttgagca
    14701 ggattaaaat tggcttaagg aatgtgatat gaaaaatgac gcagacagtt acaccgttta
    14761 aatgcaataa tcagccacgt ttctcgttaa taacaatacc agtacctggt ttgcgcaagg
    14821 cgaaggatta ttttatgaag cttaagaaca ccctcctggc gtcggcactg ctttctgcta
    14881 cggcattctc cgttaacgca gcaacagaac tgacaccgga gcaagcggca gcggttaaac
    14941 cttttgaccg tgtagtggtt accggtcgtt ttaatgctat tggcgaagcg gtgaaagccg
    15001 tttctcgtcg cgcagataaa gaaggtgccg cctcttttta tgttgtcgac acttctgatt
    15061 ttggtaacag cggtaactgg cgtgtggtcg ctgacctcta taaagccgat gctgaaaaag
    15121 cagaagaaac aagtaatcgc gtaattaacg gtgttgtcga actgccgaaa gatcaggctg
    15181 ttctgattga accgtttgac acggtcaccg tccagggctt ctatcgtagc cagccagaag
    15241 tcaatgatgc catcaccaaa gcggcaaaag cgaaaggtgc ctactctttc tacatcgttc
    15301 gtcaaatcga tgccaaccag ggcggcaacc agcgtattac tgcattcatc tataaaaaag
    15361 atgctaagaa acgtatcgtc cagagcccgg atgtgatccc ggcagattcc gaagcaggac
    15421 gtgcagctct ggctgccggt ggcgaagccg cgaagaaagt tgagatccca ggtgttgcga
    15481 ctaccgcatc accaagttct gaagtcggtc gtttctttga aacccagtca tcaaaaggcg
    15541 ggcgttacac cgtcacgctc ccggatggca ctaaagtcga agaactgaac aaagcgaccg
    15601 cagcgatgat ggtcccgttc gatagcatca aattctctgg caactacggc aacatgaccg
    15661 aagtctccta tcaggttgcg aaacgtgccg cgaagaaagg tgctaagtat taccacatca
    15721 cccgccagtg gcaggaacgt ggtaataacc tgaccgtcag cgcagatctg tataaataac
    15781 tattcagtta ctcctataag gcggcttgat gccgcctttc gcatttttag tcacaaattt
    15841 ccccgcgacc attgcatgca gacacatctc ttcgtaaaat cccgcgcttc agtggcaaac
    15901 tccattttta tgcgttttgc cctaatattt attcttctta tcacgtttta atcactggat
    15961 atcgatggaa aagaaactgg gactgagcgc actcaccgcg ctggtattaa gctcaatgct
    16021 gggcgcgggt gttttcagtc tgccgcaaaa tatggcggca gttgccagcc cggcagcact
    16081 gctcatcggc tggggtatta ctggcgctgg cattttattg ctggcctttg ccatgctgat
    16141 cctcacgcgc attcgtcccg aacttgacgg cggtatcttt acctatgccc gcgaagggtt
    16201 tggcgagcta atcggctttt gttccgcatg gggatactgg ctgtgcgcag tcatcgccaa
    16261 cgtctcctat ctggtgatcg ttttttccgc gttaagcttt tttacggaca cgccggaatt
    16321 gcgcctgttc ggcgatggta atacctggca atcgatcgtc ggtgcatcgg ctttattgtg
    16381 gattgttcac tttttgatcc tgcgcggggt gcaaaccgct gccagcatta acctggtggc
    16441 gacactggca aaattgttgc cgctgggtct gtttgttgtg ctggcgatga tgatgttcaa
    16501 actggatacc ttcaagctcg acttcaccgg acttgtactt ggcgtacccg tttgggaaca
    16561 ggtgaaaaac accatgctga tcaccctgtg ggttttcatt ggtgtggaag gtgccgtggt
    16621 tgtttctgcg cgtgcgcgta ataaacgtga tgttggcaaa gcgacactgc tggcggttct
    16681 ctccgctctg ggcgtttact tgctggtaac gctgctttcg ctgggtgtgg tggcacgtcc
    16741 tgaactggct gaaattcgta acccgtcaat ggccggtctg atggtggaaa tgatgggacc
    16801 atggggcgaa atcatcatcg ctgccagttt gatcgtttcc gtttgcggcg cgtacttaag
    16861 ctggaccatc atggcggcag aagttccgtt cctggctgcc actcataaag cattcccacg
    16921 catttttgcg cgccagaacg cacaagctgc gccatccgcc tcactgtggt tgaccaacat
    16981 ctgcgtgcaa atttgtctgg tactcatctg gctgaccggt tccgattaca acacgttgct
    17041 gaccatcgcc tcagaaatga ttctggtgcc ctatttcctg gtcggcgcgt tcttgctgaa
    17101 aatcgcaacc cgtccactgc ataaagcggt aggtgtcggt gcctgcattt atggcttatg
    17161 gttattgtac gcctctggtc caatgcactt gctgttatcc gttgttctgt atgcgccggg
    17221 gctgctggtt ttcctgtatg cgcgcaaaac gcatacacat gataatgtac tgaaccgtca
    17281 ggaaatggtg ctgattggca tgctgttaat tgcctcagtt ccggcgacct ggatgctggt
    17341 gggataacgt tcatcccatc gttggatagc aaaggagata acgatgggta aagcccagcc
    17401 cttgccaata ttaattactg gcggaggtcg tcgcatcggc ctcgccctcg cctggcattt
    17461 cattaatcaa aagcaaccgg tgattgtcag ctatcggacg cactatccag ccattgatgg
    17521 actgattaag gcaggtgcgc agtgtattca ggctgatttt tcgaccaacg acggtgtgat
    17581 ggcgtttgcc gatgaagtac taaaaagcac ccatggtctg cgtgctattt tgcataacgc
    17641 cagtgcgtgg atggcggaaa aactgggtgc gccactggcc gacgtactgg cttgcatgat
    17701 gcagatccac gttaataccc catacctgct caaccatgcg ctggaaagat tactgcgtgg
    17761 gcacggacac gccgccagcg atatcattca ctttaccgat tatgtggtgg agcgcggtag
    17821 cgacaagcat attgcgtatg ctgcaagcaa agcggcactg gataatatga cccgctcgtt
    17881 tgcccgcaag ctggcaccgg aagtgaaagt gaattctatt gctccatcgc tgatcctgtt
    17941 taatgaacat gatgatgccg aatatcgaca acaggcgctg aataaatcac tgatgaaaac
    18001 cgcgcctggc gagaaagaag tgatcgacct ggtcgattac ttacttacca gttgctttgt
    18061 caccggacgc agtttcccac ttgatggcgg tcgtcatctg cgttaatgca gctttatcca
    18121 gcatataatc aacacccatg cgcttatccc ccaacacgca acagaaccaa caaaagcggc
    18181 gggcaagcgc atcattccgg tgaaatacca cagcgacacc aggtaaacaa aatagggaat
    18241 aatcgaccac atactaaaaa tgatggttgc gcgtaaggct tcaatgccgc gttcgctggc
    18301 aacaatataa tgcgcgataa gcgcaaaggt cgggaaaagt ggaatcagcc cggcgatata
    18361 ataatttttc gtttttgcta aaacaccaat caacagcact accagcacac cgagcgcggc
    18421 tttgatttta ccttaacact tccataacaa gtcatcagta gaatacctga tgaaaacttg
    18481 tttagaaacg attgatagta agtaaaaaca gcgcggtgta ttgtgacgtt tttatatcta
    18541 ccgtgaatgt tatgaacact atcgtatttg tggaagatga tgcggaagtc ggttcactga
    18601 ttgccgcgta cctggcaaaa catgatatgc aggttaccgt agagccgcgc ggcgaccagg
    18661 ccgaagaaac cattttgcga gaaaatccgg atttggtgtt actcgacatc atgctaccag
    18721 gcaaggacgg cattaccatt tgtcgtgatt tacgcgcaaa gtggtctgga ccgattgttc
    18781 ttctaacctc tctcgatagc gatatgaacc acatcctggc actggaaatg ggtgcctgcg
    18841 actatattct caaaacgatg ccccctgctg ttttgctagc gcgtttacgt ttgcatttgc
    18901 gtcagaatga gcaagccaca ctgaccaaag gtcttcagga aacgtctctg actccctaca
    18961 aagccctgca tttcggcacg ttgaccatcg atcccatcaa ccgcgtagtc accctggcta
    19021 acactgaaat ctcgctctcg acagctgatt tcgaattatt gtgggaatta gctacccatg
    19081 ccgggcaaat catggaccgc gatgcattgc tgaaaaattt acgcggcgtc agttatgacg
    19141 gactggatcg taacgtggac gtggctattt cgcggttaag aaaaaaactg ctcgataacg
    19201 ccgcagaacc ttatctgcat taaaactgtg cgtaacaaag gctatctttt tgcgcctcat
    19261 gcatgggaat aagcgatgaa aaaactgttt atccagtttt acctgttatt gtttgtctgc
    19321 ttccttgtga tgtctctgct ggttgggttg gtgtacaaat ttaccgccga acgcgcgggc
    19381 aaacagtcgc tggatgattt gatgaacagt tcgctgtatc tgatgcgcag cgaattgcgt
    19441 gagatccccc cacacgactg gggtaagacg ctgaaagaga tggatttaaa tctctctttc
    19501 gatctgcgtg tcgagccact gagtaaatac catcttgatg atatttccat gcaccgactg
    19561 cgtggcggcg aaattgtcgc cctggacgat cagtacacgt ttttgcagcg tatcccgcgc
    19621 agccactacg tgctggcagt tggtcctgtt ccttatcttt attatctcca tcagatgcga
    19681 ttgctggata tcgccctgat cgcttttatt gctatttccc tcgcctttcc ggtgtttatc
    19741 tggatgcgtc cgcactggca ggatatgtta aaactggaag cagcggcgca acgatttggc
    19801 gatgggcatc tctatgagcg tatccacttt gatgagggtt cgagctttga acgacttggt
    19861 atcgcattta accagatggc ggacaatatc aacgccttga ttgccagcaa aaaacagctt
    19921 attgacggta tcgctcacga actgcgaaca ccgttagtgc gcctgcgtta tcgactggag
    19981 atgagcgata acctgagcgc cgccgagtcc caggcgttga atcgtgatat cagtcaactt
    20041 gaagctttaa ttgaagagct gctgacttat gcccgactcg atcgcccaca aaacgagctt
    20101 catcttagcg aaccagacct gccgttgtgg ctgtcaacgc atctggcaga tattcaggca
    20161 gtaacgcccg ataaaacggt acggataaaa acgctcgtgc aaggccatta tgcggcgttg
    20221 gatatgcgct taatggagcg cgtgctggat aatttgctca ataacgccct gcggtactgc
    20281 cattcaacgg ttgaaaccag cctgctactg tcggggaata gagcgacatt aattgttgag
    20341 gatgatggcc cagggattgc cccagaaaac cgcgaacata tctttgaacc ttttgttcgc
    20401 ctcgatccca gccgggatcg ctcaaccggc ggctgcgggc tggggctggc aattgtccac
    20461 tctatagcac tggcaatggg cggtacggtt aattgtgaca ccagcgaact gggtggtgct
    20521 cgcttctcgt ttagctggcc gttatggcat aacatcccgc aatttacctc tgcctgacac
    20581 tacgcgcacg atggtcaagt caccaccatt gtgctataaa ttaaggatgt tgtaactaaa
    20641 gtggttaata ttatggcgcg ttacgatctc gtagaccgac tcaacactac ctttcgccag
    20701 atggaacaag agctggctgc atttgccgct catcttgagc aacacaagct attggttgcc
    20761 cgcgtgttct ctttgccgga ggtaaaaaaa gaggatgagc ataatccgct taatcgtatt
    20821 gaggtgaaac aacatctcgg caacgacgcg cagtcgctgg cgttgcgtca tttccgccat
    20881 ttatttattc aacaacagtc cgaaaatcgc agcagcaaag ccgctgtccg tctgcctggc
    20941 gtgttgtgtt accaggtcga taacctttcg caagcagcgt tggtcagtca tattcagcac
    21001 atcaataaac tcaagaccac gttcgagcat atcgtcacgg ttgagtcaga actccccacc
    21061 gcggcacgtt ttgaatgggt gcatcgtcat ttgccggggc tgatcaccct taatgcttac
    21121 cgctcgctca ccgttctgca cgaccccgcc actttacgtt ttggctgggc taataaacat
    21181 atcattaaaa atttgcatcg tgatgaagtc ctggcacagc tggaaaaaag cctgaaatca
    21241 ccacgcagtg tcgcaccgtg gacgcgcgag gagtggcaaa gaaaactgga gcgagagtat
    21301 caggatatcg ctgccctgcc acagaacgcg aagttaaaaa tcaaacgtcc ggtgaaggtg
    21361 cagccgattg cccgcgtctg gtacaaagga gatcaaaaac aagtgcaaca cgcctgccct
    21421 acaccgctga ttgcactgat taatcgggat aatggtgcgg gcgtgccgga cgttggtgag
    21481 ttgttaaatt acgatgccga caatgtgcag caccgttata aacctcaggc gcagccgctt
    21541 cgtttgatca ttccacggct gcacctgtat gttgcagatt aacgcccggc tttcatactg
    21601 ccgaccatct gttctggccg tacccagctg tcaaactcgg cttcgctaag ataccccagc
    21661 gcaagggccg cagcttttaa ggtcagccct tctttatgcg cttttttggc gatctccgcg
    21721 gctttgtcat aaccaatgtg ggtgttaagc gcagtcacca gcatcagcga ttcattgagt
    21781 aattgattga ttcgctcacg attcggttca atacccactg cgcagtgttt gttaaaactt
    21841 tccatgccat ctgccagcaa gcgcaccgat tgcaggaaat tatggatcac catcggacgg
    21901 aagacgttca gttcaaagtt accggaagcg ccacccatgt tgatcgccac gtcgttcccc
    21961 attacctgac agcagagcat ggttaatgct tcacactggg ttgggttcac tttccccggc
    22021 atgattgagc tgcccggctc attttccggg attgagattt caccaattcc gcagcgcggg
    22081 ccagaggcca gccagcggac atcattggcg attttcatca gtgacgcagc caaccctttc
    22141 aacgcgccgt gcgcctgaac cagggcatca caggtcgcaa gcgcttcaaa tttgttcggc
    22201 gcggtaacaa acggtgcaca ggtaatgact gccagttcat ctgctacgcg acgcgcatac
    22261 tccggatggg tatttagtcc agtacccacc gctgtaccgc caagagccag ttccgctacg
    22321 tgaggcaggc tgtattcgat atgtttgaga ttatgctcga gcatcgctac ccagccggaa
    22381 atctcctgcc ctagtgttag cggcgtggcg tcctgcaagt gggttcgacc gattttgacg
    22441 atatcggcaa atgcacgcga tttttcactc agtgtctgcg tcagggtttt aagctgcgga
    22501 atgagttgct tgcgcagcgc cagcagcgcc gcaacgtgca tcgccgtcgg aaagacatcg
    22561 ttggaacttt ggcttttgtt cacgtcgtcg ttagggtgaa ctttacgttc catcccgcgc
    22621 acgccgccga gtaattcact ggcccggtta gccagcactt cgttcatgtt catgttactt
    22681 tgcgtgccgg agccggtctg ccagatagcc agcgggaatt cgtcgtcatg ctgtcctgcc
    22741 agtacttcat ccgccgcctg ccgaatggcg ctcgctttct cttcagacaa caagcctaaa
    22801 tcttcattaa cttttgccgc tgcgcgcttg gttagcgcca gcgcatgaat cagtgaggtg
    22861 ggcattttct ccgtcgaaat gcggaaatgc tccagcgagc gttgagtttg tgcgccccac
    22921 agcttatctg ccgggacatc aatcgccccc atcgaatctt tttcgctgcg tactgtattc
    22981 atggcctgct cctcacctgt ttaatttttt ctttctgttt tgctttcgct aagcaacttt
    23041 tagctcactt attatttact atttgataac aagtgtttgg tctttcgtgc catgtaaaaa
    23101 aaccgccccg aagggcggct ctgtttattt cacacagcga gtgcactgtg tgagttgtat
    23161 ctgctggaag aagtcatttc ctttatcatc cacaaggata aacgccggga aatcttccac
    23221 ttcaattttc cagatggctt ccattcccag ttccggatat tcaacacatt ccaggctctt
    23281 aatacttccc tgcgccaata cagcggccgg accaccgata ctgccaaggt agaagccgcc
    23341 gtgtttttta caggcatccg tcacctgctg gctgcggttg cctttcgcca gcatgatcat
    23401 acttccgccc tgcgcttgca gttgatcgac ataagaatcc atccgtccgg cggtcgttgg
    23461 gccaagagaa ccggaggcat aaccttccgg cgttttggcc ggacccgcgt agtaaatcgg
    23521 atgatcttta atgtactgcg gcagcccttc accgttatcc atccgctctt tcagtttggc
    23581 gtgagcaata tcacgaccga cgataatcgt gccgttaagc gataagcgtg tagaaacggg
    23641 atactgcgac aactgtgcga ggatctcttt catcggacgg ttaaggtcaa cgcgcaccgc
    23701 ttcgccttct cccgctttac gcagctcttc agggatatat ttacctggat tatgttccag
    23761 tttttcgatc cagatcccct gacggttgat cttcgctttg atattacggt cagcagagca
    23821 ggaaacaccc ataccaaccg gacaggatgc gccgtgacgt ggcaggcgaa tcacgcgtat
    23881 gtcgtgagcg aagtatttac caccaaactg cgcacccaga ccgagatttt gcgcttcgat
    23941 cagcaattct ttttccagtt ccacatcgcg gaatgcctga ccgtgctcat tcccttccgt
    24001 tggcagttcg tcatagtatt tagcggaagc cagtttcact gttttaaggt tagtttctgc
    24061 agaagttcca ccaattacga acgcaatatg atacggagga caggccgccg tacccagcgt
    24121 gcgcatcttc tcaaccaggt aatttttcag tttccccggc gttagtaacg ctttggtttc
    24181 ctgatagaga tacgtcttgt ttgccgaacc accacctttg gcgatacaga ggaatttgta
    24241 ctcgtcgcca tcaacggcat aaagatcgat ctgcgctggc agattggtgc cggtattcac
    24301 ctctttatac atatccagcg gcgcgttttg cgagtagcgc aaattatctt cgatataagt
    24361 gttatagaca ccgcgcgcca gcgccgcttc atcacagcca ccggtccata cacgctgccc
    24421 ttttttacca acaataatcg cagtgccggt atcctgacag gttggcagaa cacctttcgc
    24481 cgcgatgtcg gagttgcgca ggaattgcag cgccacatat ttatcatttt cgctggcttc
    24541 cgggtcacgc agaatgtcgg ccacctgttg ttggtgagcc ggacgcagca taaatgacgc
    24601 atcatgaaac gcctgacgcg ccaacagagt taacgcttcg ggtgcgactt tcaaaatctc
    24661 ctgcccttca aattcagata cgctaacgtg ttcgctggtt agcaggtaat actcagtatc
    24721 atcttttttg agtggaaaag gagcctgata atgaaagggt ttgtttgaca ttgttctctc
    24781 acttactgcc tggtttggtt atgctctggg cgggtgttcc gttgccctgt taaaagcgag
    24841 taacaatatc ctacacactt ttttaacaaa aactgagact agtacgactt tttgcggctc
    24901 caggttactt cccgtaggat tcttgcttta atagtgggat taatttccac attaaaacag
    24961 ggattgatca tgcaaaaact cattaactca gtgcaaaact atgcctgggg cagcaaaacg
    25021 gcgttgactg aactttatgg tatggaaaat ccgtccagcc agccgatggc cgagctgtgg
    25081 atgggcgcac atccgaaaag cagttcacga gtgcagaatg ccgccggaga tatcgtttca
    25141 ctgcgtgatg tgattgagag tgataaatcg actctgctcg gagaggccgt tgccaaacgc
    25201 tttggcgaac tgcctttcct gttcaaagta ttatgcgcag cacagccgct ctccattcag
    25261 gttcatccaa acaaacgcaa ttctgaaatc ggttttgcca aagaaaatgc cgcaggtatc
    25321 ccgatggatg ccgccgagcg taactataaa gatcctaacc acaagccgga gctggttttt
    25381 gcgctgacgc ctttccttgc gatgaacgcg tttcgtgagt tttccgagat tgtctcccta
    25441 ctccagccag tcgcaggtgc acatccggca attgctcact ttttacaaca gcctaatgcc
    25501 gaacgtttaa gcgaactgtt cgccagcctg ttgaatatgc agggtgaaga aaaatcccgc
    25561 gcgctggcga ttttaaaatc ggcgctcgac agtcagcagg gtgaaccgtg gcaaacgatt
    25621 cgtttaattt ctgaatttta cccggaagac agcggcctgt tctccccgct gttactgaat
    25681 gtggtgaaat tgaatcctgg cgaagcgatg ttcctgttcg ctgaaacacc gcacgcttac
    25741 ctgcaaggcg tggcgctgga agtgatggcg aactccgata acgtgctgcg tgcgggtctg
    25801 acgcctaagt acattgatat tccggaactg gttgccaatg tgaaattcga agccaaaccg
    25861 gctaaccagt tgttgaccca gccggtgaaa caaggtgcag aactggactt cccgattcca
    25921 gtggatgatt ttgccttctc gctgcatgac cttagtgata aagaaaccac cattagccag
    25981 cagagtgccg ccattttgtt ctgcgtcgaa ggcgatgcaa cgttgtggaa aggttctcag
    26041 cagttacagc ttaaaccggg tgaatcagcg tttattgccg ccaacgaatc accggtgact
    26101 gtcaaaggcc acggccgttt agcgcgtgtt tacaacaagc tgtaagagct tactgaaaaa
    26161 attaacatct cttgctaagc ttattaaagg cttataacac cttcaggcgg ccagtccgcc
    26221 tgatttcatt ttatggataa tcattatgaa taaatcgctg gtagcggtag gcgtcattgt
    26281 tgcgctaggc gtagtctgga caggcggcgc atggtataca ggcaagaaga ttgaaaccca
    26341 tctcgaagac atggtcgcgc aggcgaacgc gcaactaaaa ctgacagctc ctgaatccaa
    26401 cctggaagtg agttatcaaa actatcatcg cggcgtattc agcagtcagt tgcaactgtt
    26461 ggtgaaaccc attgccggga aagaaaatcc gtggattaaa agcggtcaga gcgtcatctt
    26521 caacgaatcg gttgatcatg gtcccttccc gcttgcccag cttaaaaaac tgaacctgat
    26581 cccgtcgatg gcatcaattc aaaccacgct ggttaataac gaagtaagca aaccactgtt
    26641 tgatatggca aaaggtgaaa cgccttttga gattaactcg cgcattggtt acagcggtga
    26701 ttccagttcc gatatttcgc tcaagccact gaattacgag caaaaggatg aaaaagtcgc
    26761 ctttagcggc ggcgagttcc agttaaatgc tgacagagac ggcaaagcca tctccctttc
    26821 cggggaggcg caaagtggtc ggatagacgc agttaacgaa tacaaccaga aagtgcagtt
    26881 gacctttaat aatctgaaaa ccgacggttc cagcacgctg gcaagttttg gtgagcgcgt
    26941 aggaaaccaa aaactgtcac tggaaaaaat gaccatttca gtggaaggca aagaactggc
    27001 actgctggaa ggcatggaga tcagcggtaa atcggatctg gtcaatgacg gtaaaacgat
    27061 caatagccaa ctggattact cgctaaacag cctgaaggta cagaatcagg atctgggcag
    27121 cggcaagctg actttaaaag tcggccagat tgatggtgaa gcctggcatc agttcagcca
    27181 gcaatataac gcgcaaactc aggcgctgct ggcacagcca gaaattgcca acaatcccga
    27241 actttatcag gagaaagtga cggaagcctt ctttagcgcc ctgccgctga tgttgaaagg
    27301 cgatccggtg attactgtcg cgccgctaag ctggaaaaac agtcagggtg aaagtgcgct
    27361 gaatctgtcg ctgttcctga aagatccggc aacgactaaa gaagcgccgc aaacgctggc
    27421 gcaggaagta gatcgttcgg ttaaatctct ggatgcgaaa ctgaccattc cggtggatat
    27481 ggcaactgag tttatgactc aggtagcgaa gctggaaggt tatcaggaag atcaagcgaa
    27541 aaaactggcg aaacagcaag ttgaaggtgc atcagcaatg gggcagatgt tccgtctgac
    27601 caccttgcag gacaatacca tcaccaccag cctgcaatat actaacggtc agataacgtt
    27661 aaatgggcag aaaatgtcgt tagaagattt tgttggcatg tttgcgatgc cagctcttaa
    27721 cgttccggct gtaccggcaa ttccgcagca gtaattcacg gccaaaacgc cggagaattt
    27781 tcacttcggc gttttcatct gaaaacaaac cagagattgc ctgatgcgct acgcttatca
    27841 ggcctacctt ttcctgcaat actttgaatt tatgagtttt tgtaggctgg ataaggcgtt
    27901 tacgccgcat ccggcataaa aaacgcgcac tttgtcaaca atctgaaacg ccggaggttt
    27961 ttctctccgg cgttatttct tacttcagca taaagtcata ctgaatactc attttcacat
    28021 cccagtaatt caggcgatcg cccagcgtac tgccatcgcg cttttcccgc cctttatcgg
    28081 cagcaagaaa gaaattcatc ccttgtaacg gcccttcgct gacccgatag ttcataccac
    28141 ctaagatacc cgtgccttca atccgcacat ctcgtttact ggattcatag ccgcgagtca
    28201 caatcggtgc aaatgtcagt gtaaattgcg gcgttaaacg atagttgacg cccaattgcc
    28261 aggactgcat tccttcacgg ttacgatcaa gacttaatga aaagggatag ccaatgtcgg
    28321 tatccaccac ggcaccattc attgcatggc gcaaataacc accactgcca aatacgcttc
    28381 cccacggcgc attccaggtc aattgtccac taaccaacgc cgtttcattg ggctggctgg
    28441 tattacgact taaaccttcg agctgcgcgt aaaagccgag caactcccct ttcaacgtat
    28501 aattcgggtc gagcggtatg cttttctcga gaatactttg tatctgattg gaaacatctt
    28561 tctgtaccga tgcccccaga gaatagcgac tggtatcatc atgccaactg gcggctaaaa
    28621 aagaacgtgc gcgatgttta tcgtattttc ccgtctggct gttatacacc ccttcatcac
    28681 gaataccggt tgttgaatca gtccataaaa atactcgtga acgccacatt ccccacagat
    28741 caatatttgg tgttaaagcc caataaccta atgctccttc agaaactgtc ggtaatgtac
    28801 gttgcgaact gccagccatt aacggaatac gataaggtaa gctaccataa ctataatccg
    28861 tcccgaagcg acctaattta agcaggctat tttcacaggg ttttattttt actgccccca
    28921 gggcaaagcc aaaactttta tcaccatcaa gataccaccg ggtactcata tcagcacgag
    28981 cacctaattt ataaacataa tatgccccgc cttcaacgcc aataatattg gaataataac
    29041 cactattgaa atcgaacaat cctctctgta cccatgcgta aatatctctt cccgcgccag
    29101 cactgggctt atccgccctg cgcaattcgt ttttcagtct gatgcgtagt cccgcttcat
    29161 cgtcagccag ttgcgccgca taagcagagg taaggccaga ggcagccgtc aggcaaataa
    29221 cggccatggc cactattttt ctcatagttg cacctttgat gttctgatta tttttattga
    29281 atattaatta gtgatatcgc tgattaattg ctgctgcact tttttacgat tatcaatttc
    29341 aaccacgatt tctttgaatt ttttatccgt gagcggataa aaccagataa taacgaatgc
    29401 cagtagcata aatccgcaag gtactaaggc aattgatgtg cggatgccca taataacttc
    29461 cggcgtttgc acctgattgg cgatatatcc gcttaatccc aaaataaagg caggaattga
    29521 acctccgatt gcctgaccgc atttacgggt aaatgagaat agtgaatagg tgagcccttc
    29581 aattcgcacg ccggtcaggt attcaccgta ttctacggta tcagcttcca gtgcccacat
    29641 cacagtcata gtaacgccct gaccaattga agcgatggcc aacgcaacca acgccaccgg
    29701 cagtgaccag acggaaaccc agaagaatag cagataaccg caggttccca gcaaagcgcc
    29761 aatcaggaag gtattctttt taccgctcct cgcgaccatc cccggcacca gcggtgccga
    29821 tgccacagta ccaaccaagt tttgcaccag taccagcaca gtgaacagcc cggtatcatt
    29881 taacacatag cgctcgtaga acaacgacga ggcgctgacc gcaaaggtcg aaatcagcac
    29941 acacagcgca ccgatgcaca acataaacag cgggcgattc cgtttcaggg tttgcagact
    30001 gatcttcaat gacggctgcg caacgatacg taccacattc tcacgcgtcg atttgaagca
    30061 gatgaagtaa agcaccattc cggcaatcgc cagcacaatc gtccagaaat ggtataccga
    30121 caccatctct tccgggctgg agttcttaat gctcggccct atcagaaatg ccaggcagac
    30181 aaaggtcaat gaagcggcaa tcccacgagc cgcgcccaga cgggcgcggg attgtggttg
    30241 ttgggtcatc gcggtagcaa gtgaaccata aggaatattc accaggctat agcaaagccc
    30301 gaggcccatg taggtcaaat atgcatacac tactttgctg ctatggctcc agtcggtcgg
    30361 tacccagaat accagcacgc tgaagatcat taacggcgca gtaccgaaga gtaaaaacgg
    30421 gcggaatttt ccccagcggg tattcacact gtccaccact cgtccggcaa agacgtcggc
    30481 gaaggcatcg aatacccgca ccaataacag catggtgccc gccgcagcgg caccgacgca
    30541 agcgacgtcg gtgtagtaac tcaacaggaa gagcgccccc attgcgaagg cgaagttatt
    30601 ggcgacgtca ccgaggctgt agccgacgat ggtgcgccag gagagttgtt gattcattgt
    30661 ttgcctccct gctgcggttt ttcaccgaag ttcatgccag tccagcgttt ttgcagcaga
    30721 aaagccgccg acttcggttt gcggtcgcgg gtgaagatcc ccttcttgtt accgccaacg
    30781 cgcaatatgc cttgcgaggt cgcaaaatcg gcgaaattcc atacctgttc accgacgccg
    30841 gcgctgacgc gatcaaagac gcggtgatac atatccagcc atgcacactg atactcttca
    30901 ctccacatgt cggtgtacat tgagtgcagc ccggctaacg tatccacgcc gtattcggtg
    30961 atgataatcg gctgatgcag tttctcctgc caggctagaa gttctttttc cagtaccttc
    31021 tctgccgttt ccaaatcgcc gctttggaca taccatccgt aataacggtt caggcacagc
    31081 acatcaaaga gatcgctgat ggtatcggtg tgagcgtcgc agaacattac attgacgcag
    31141 gtgatcggac gcgtcgggtc gagtttacgc gttgcttccg ccagtggcgc gaaatattct
    31201 cgtgcacctt gcggacgggt atccggttcg ttggcaatac tccacatcac cacgcttggg
    31261 tggtttttgt cacgcgctat cagctcttta atcgcctgta aatgcgcctg ctgagtttcc
    31321 ccgttgacag cctcttcgct gtacagttct ttcggcttgt tgcccgcttc gaaaccaatg
    31381 cctaaagaga ggttaaagcc gacagctgca gtttcatcaa tcaccacgat gccatgttca
    31441 tctgcccact cgagcatctc ttcagcgtaa gggtaatgcg aggttcggta ggagttggcc
    31501 ccaatccagt ccattaatgc gtgatcgtgc accatcagca cgttatcgaa tcctttgcct
    31561 cgcaaatccg catcttcatg acgaccaaag ccagtaaagt agaacggttt gtggttgttc
    31621 gtccttcact gccactgacc ggatgccgac gcgcagcggg tagatatcac actctgtctg
    31681 gcttttggct gtgacgcaca gttcatagag ataaccttca cccggttgcc agaggtgggg
    31741 attcaccact tgcaaagtcc cgctggtacc ttgtccagtt gcaaccacct gctgatccgc
    31801 atcacgcagt tcaacgctga catcaccatt tgctaccacc tgccagtcaa cagacgcgtg
    31861 gttacagtct tgcgcaacat gcgtcaccac ggtgatatcg tccacccagg tgttcggcgt
    31921 ggtgtagacc attacgctgc gatggatccc ggcatagtta aagaaatcat ggaagtaaga
    31981 ctgctttttc ttgccgtttt catcggtaat caccattccc ggcgggatag tctgccagtt
    32041 cagttcgttg ttcacacaaa cggtgatacg tacacttttc ccggcaataa catacggcgt
    32101 gacatcggct tcaaatggcg tatagccgcc ctgatgctcc atcacttcct gattattgac
    32161 ccacactttg ccgtaatgag tgaccgcatc gaaacgcagc acgatacgct ggcccgccca
    32221 acctttcggt ataaagactt cgcgctgata ccagacgttg cccacataat tacgaatatc
    32281 tgcatcggcg aactgatcgt taaaactgcc tggcacagca attgcccggc tttcttgtaa
    32341 cgcgctttcc caccaacgct gatcaattcc acagttttcg cgatccagac tgaatgccca
    32401 caggccgtcg agttttttga tttcacgggt tggggtttct acaggacgta acataaggga
    32461 ctcctcattc aaataaaaat actggtcaac ctttaatctg attagttaac caattctgtg
    32521 ttgcacacag ttatctgtga gaaacccatg gaagagaaat ggaaataaaa agaactgaca
    32581 ccgcgatcaa aaaaccaaaa tctgcaatac gcacagcgtg atatagatcg cattaatctt
    32641 taaaacagcg ttgacagaag ccataaaata agcaatcgaa cgctcgttcg agcatcttga
    32701 gaacgataaa ctctcatgac atatggctac ggtgaatatt ttggggggga attctccgat
    32761 cccggctaga ttgtattttg tacagggaaa ttaaagtatg aaacgcgggc acagttatca
    32821 ccagcccgcg aaatgagagg atcaggatgc ggttaagata ccgccaatca tcgcgcgtaa
    32881 tccctgagca aacgacgctt cacgcgccag atcttccgct tcgatatcgg ccagtacacc
    32941 gtaggtcaga tccagcagta aacgtgaagt cattgacgtg ttgatgtctg ggcttatctc
    33001 gcctttttgc tgggcatcac gcatccgctg ggcaacaaat tcagtgatcg tcatatgctt
    33061 atttttaagc atggcggcga cctgtgggtt acgcatccct tccgccataa tttcgaccac
    33121 cagcgcccgt tgcccaaagg cttcatgggt gagagagaca atagactcga ccatatagtc
    33181 aacaaaatga atcccttcaa tcggttcccg gaaacgggcc agcgccctct cctggtcctg
    33241 taagataatc gcctgaatca aggcttcttt ggagatgaaa tggtgataaa gcgtcccggg
    33301 actaatggcg caggatttac agatggcttt catcgaggca ctgtgaaatc cattttctga
    33361 aaaaatctct ctggcagcat tgaggatccg ggtccgtgtc ggttgtgctt cagtctgcat
    33421 gttatccatc attaaaccat tatagttagc gtgaataact agtttaccgc gttgtcaggt
    33481 gagcgcctat cattgctgtt gaatagtgcg aaggcacact ctattcgtat gcttttcatc
    33541 cagctattgg ttaaccaata gccgtgattt ggctgccagt tgtttagcag agacgttgat
    33601 gaattgcaca cgctatcccg gtaaacaaca catacggctg tttaccggtt cgttagtgta
    33661 ttaattgagc tcctgtaccc caccaccgga gacggtgaga atttgtccgc ttacccagct
    33721 ggctgcaggc gagcaaagga acagcgccgc gttagcaata tcttgcggtt gacccagacg
    33781 tctgatcggc gtgtgctgta acattttttg ttcaatttct ggtgtaataa cggatttcag
    33841 ggcatcggtt aatattgccc ccggcgcaat gccattcacc cgaatatttt tttcacccag
    33901 gtcaaatgcc atatttctga ccagatgact ggccgcagct ttagatgatg cataggaagt
    33961 catgtttata tttttatttt ctgccgccat agaagtgatg gtcagaataa cgccaccgcc
    34021 atttttttcc atttctggcg caacaagttg tgacagatgg aaaaaagaaa acacattcag
    34081 ttcataagca cggcgaaaat ccgccattgg catatcaaac ggtttaggtc caccgccacc
    34141 ggcgttgtta accagaatat caactttacc cagcttactg atagcaaagt ctgccagtgc
    34201 agagagttcc tgttcggaag taatatcaca acggcaggca aatgcctgcc cccccagttg
    34261 ttgaatttcg tctacaacat ggttagctgc gtcggcgtta atatcactga ccaccacaga
    34321 tgcgccagct gtcgcgaatg taatggcgat ttctttacca atacctgcac ccgcacctgt
    34381 gatgatggcg cattttccgt cgagtctcag gttgtcagaa ttaaacacat aaacctcctg
    34441 tgaacttcag taaaaataaa aggcaccgcc t
//
LOCUS       DAZPEB010000020        34002 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZPEB010000020 DAZPEB010000000
VERSION     DAZPEB010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34002
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.020"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(314..526)
                     /gene="rpmE"
                     /locus_tag="ACHURJ_000877"
     CDS             complement(314..526)
                     /gene="rpmE"
                     /locus_tag="ACHURJ_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFR1279930.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            729..2927
                     /gene="priA"
                     /locus_tag="ACHURJ_000878"
     CDS             729..2927
                     /gene="priA"
                     /locus_tag="ACHURJ_000878"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFR1279931.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNACPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPMFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWILGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            3083..4108
                     /gene="cytR"
                     /locus_tag="ACHURJ_000879"
     CDS             3083..4108
                     /gene="cytR"
                     /locus_tag="ACHURJ_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFR1279932.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4200..5159
                     /gene="ftsN"
                     /locus_tag="ACHURJ_000880"
     CDS             4200..5159
                     /gene="ftsN"
                     /locus_tag="ACHURJ_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFR1279933.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5252..5782
                     /gene="hslV"
                     /locus_tag="ACHURJ_000881"
     CDS             5252..5782
                     /gene="hslV"
                     /locus_tag="ACHURJ_000881"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFR1279934.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5792..7123
                     /gene="hslU"
                     /locus_tag="ACHURJ_000882"
     CDS             5792..7123
                     /gene="hslU"
                     /locus_tag="ACHURJ_000882"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFR1279935.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7190..8116
                     /gene="menA"
                     /locus_tag="ACHURJ_000883"
     CDS             7190..8116
                     /gene="menA"
                     /locus_tag="ACHURJ_000883"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFR1279936.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDRPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFILGIFLSQWAA"
     gene            8209..8694
                     /gene="rraA"
                     /locus_tag="ACHURJ_000884"
     CDS             8209..8694
                     /gene="rraA"
                     /locus_tag="ACHURJ_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFR1279937.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8779..9024)
                     /gene="zapB"
                     /locus_tag="ACHURJ_000885"
     CDS             complement(8779..9024)
                     /gene="zapB"
                     /locus_tag="ACHURJ_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFR1279938.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9450..10295
                     /gene="glpF"
                     /locus_tag="ACHURJ_000886"
     CDS             9450..10295
                     /gene="glpF"
                     /locus_tag="ACHURJ_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFR1279939.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVKEKETTTPSE
                     QKASL"
     gene            10318..11826
                     /gene="glpK"
                     /locus_tag="ACHURJ_000887"
     CDS             10318..11826
                     /gene="glpK"
                     /locus_tag="ACHURJ_000887"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFR1279940.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            11961..12971
                     /gene="glpX"
                     /locus_tag="ACHURJ_000888"
     CDS             11961..12971
                     /gene="glpX"
                     /locus_tag="ACHURJ_000888"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFR1279941.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAVLGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            13068..13814
                     /gene="fpr"
                     /locus_tag="ACHURJ_000889"
     CDS             13068..13814
                     /gene="fpr"
                     /locus_tag="ACHURJ_000889"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFR1279942.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13819..14247)
                     /gene="uspD"
                     /locus_tag="ACHURJ_000890"
     CDS             complement(13819..14247)
                     /gene="uspD"
                     /locus_tag="ACHURJ_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFR1279943.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14274..14573)
                     /locus_tag="ACHURJ_000891"
     CDS             complement(14274..14573)
                     /locus_tag="ACHURJ_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFR1279944.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(14785..15225)
                     /locus_tag="ACHURJ_000892"
     CDS             complement(14785..15225)
                     /locus_tag="ACHURJ_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFR1279945.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     CFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15326..15925
                     /locus_tag="ACHURJ_000893"
     CDS             15326..15925
                     /locus_tag="ACHURJ_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFR1279946.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            16033..16800
                     /gene="tpiA"
                     /locus_tag="ACHURJ_000894"
     CDS             16033..16800
                     /gene="tpiA"
                     /locus_tag="ACHURJ_000894"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFR1279947.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(16855..17610)
                     /gene="cdh"
                     /locus_tag="ACHURJ_000895"
     CDS             complement(16855..17610)
                     /gene="cdh"
                     /locus_tag="ACHURJ_000895"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFR1279948.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(17717..18706)
                     /gene="sbp"
                     /locus_tag="ACHURJ_000896"
     CDS             complement(17717..18706)
                     /gene="sbp"
                     /locus_tag="ACHURJ_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFR1279949.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVMRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(19026..19988)
                     /gene="pfkA"
                     /locus_tag="ACHURJ_000897"
     CDS             complement(19026..19988)
                     /gene="pfkA"
                     /locus_tag="ACHURJ_000897"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFR1279950.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGSMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20169..21071)
                     /gene="fieF"
                     /locus_tag="ACHURJ_000898"
     CDS             complement(20169..21071)
                     /gene="fieF"
                     /locus_tag="ACHURJ_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFR1279951.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMIDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21219..21755)
                     /gene="cpxP"
                     /locus_tag="ACHURJ_000899"
     CDS             complement(21219..21755)
                     /gene="cpxP"
                     /locus_tag="ACHURJ_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFR1279952.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQRS
                     TQRSTQRSTQSHMFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENF
                     DENAVRAQAEKMANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQ
                     WQKSSSLKLLSSSNSRSQ"
     gene            21905..22603
                     /gene="cpxR"
                     /locus_tag="ACHURJ_000900"
     CDS             21905..22603
                     /gene="cpxR"
                     /locus_tag="ACHURJ_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFR1279953.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            22600..23973
                     /gene="cpxA"
                     /locus_tag="ACHURJ_000901"
     CDS             22600..23973
                     /gene="cpxA"
                     /locus_tag="ACHURJ_000901"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFR1279954.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(24079..24753)
                     /gene="yiiM"
                     /locus_tag="ACHURJ_000902"
     CDS             complement(24079..24753)
                     /gene="yiiM"
                     /locus_tag="ACHURJ_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFR1279955.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(24902..25886)
                     /gene="kdgT"
                     /locus_tag="ACHURJ_000903"
                     /pseudo
     CDS             complement(24902..25886)
                     /gene="kdgT"
                     /locus_tag="ACHURJ_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
     gene            complement(26146..26766)
                     /gene="sodA"
                     /locus_tag="ACHURJ_000904"
     CDS             complement(26146..26766)
                     /gene="sodA"
                     /locus_tag="ACHURJ_000904"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFR1279956.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            27050..28084
                     /gene="rhaT"
                     /locus_tag="ACHURJ_000905"
     CDS             27050..28084
                     /gene="rhaT"
                     /locus_tag="ACHURJ_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HFR1279957.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLLNTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     IIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(28081..28929)
                     /gene="rhaR"
                     /locus_tag="ACHURJ_000906"
     CDS             complement(28081..28929)
                     /gene="rhaR"
                     /locus_tag="ACHURJ_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFR1279958.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVSFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(29003..29839)
                     /gene="rhaS"
                     /locus_tag="ACHURJ_000907"
     CDS             complement(29003..29839)
                     /gene="rhaS"
                     /locus_tag="ACHURJ_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFR1279959.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            30127..31539
                     /gene="rhaB"
                     /locus_tag="ACHURJ_000908"
     CDS             30127..31539
                     /gene="rhaB"
                     /locus_tag="ACHURJ_000908"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFR1279960.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRSNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACRETAQPIPESDAELAQLRGEDFSQLHIVGGGCQNTLLNQ
                     LCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDFRQVVSTTANLTTFTPNPDS
                     EIAHYVAQLHSTRQTKELCA"
     gene            31536..32795
                     /gene="rhaA"
                     /locus_tag="ACHURJ_000909"
     CDS             31536..32795
                     /gene="rhaA"
                     /locus_tag="ACHURJ_000909"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFR1279961.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNFHAIYLESD
                     TPVSRDQIKPEHFKNWGEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISKKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDCDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKEILSQRG"
     gene            32878..33702
                     /gene="rhaD"
                     /locus_tag="ACHURJ_000910"
     CDS             32878..33702
                     /gene="rhaD"
                     /locus_tag="ACHURJ_000910"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFR1279962.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YKDNVHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            33712..>34002
                     /gene="rhaM"
                     /locus_tag="ACHURJ_000911"
     CDS             33712..>34002
                     /gene="rhaM"
                     /locus_tag="ACHURJ_000911"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFR1279963.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSEL"
BASE COUNT         8333 a         8871 c         8819 g         7979 t
ORIGIN      
        1 gcggattgac cggatggccg gacgtcaccc cgccggggca cactgaacag gccacgccgg
       61 tgggagcacc aatgcgcacc ccggctgaac cctgaacaat gctaccgcca tactgcgtca
      121 tgtcgccctg acgcgccgcc ggttttccgc tcatcgtaaa atccttttga aataaataag
      181 aaccagaagc ctagcgccag tcaacaacag gaaatgataa tgagttaaca ccccggaatt
      241 tttcagaatt tataaaaggc aaaattcagg cacaaaaaaa gcgccgtgcg gcgctttttt
      301 cggaaatccg gtcttatttg ctgcccggga tgttgaaacg cttgttgaag cggtcaacac
      361 ggccaccggt agcaacatca cgctgtttgc cagtgaagaa cgggtggcac ttgctgcaca
      421 cgtcgaggtt caggtcatga ccaacagtgg agcggatttt cattacgtta ccgcaagagc
      481 agctagcagt aatttcttcg tatttcgggt gaatatcttt tttcatggga aaacctcggt
      541 ttaaggccgc gtcgctcttc cagccctaac gccagacacc acgcgatgtt aaaagtatag
      601 cttcaatacg atcatttcgt acgaaggcgc gaaatcatac agaaattaac cagcgtatgc
      661 aaactgatcc gcactcttct acggcaatgt gtatactaac ccaccgaatt tcaagtcagg
      721 atgatgctat gcccgttgcc cacgttgcct tgcccgttcc gcttcctcgt acctttgact
      781 atttgctgcc agaaggcatg acggttaaag ccgggtgtcg cgtgcgcgtg ccgtttggca
      841 aacagcagga gcgcatcggg gttgtggtgt cagttagcga tgtcagtgag ctgccgctta
      901 acgagctgaa agcggtagtg gaagtgctgg acgttgagcc ggtatttact cactccgtct
      961 ggcggttact gctctgggcg gcagattact atcatcatcc gattggcgat gtgctgtttc
     1021 atgccttgcc gattttactg cgccaggggc ggcctgcggc gaacgcgccg atgtggtact
     1081 ggtttgccac tgaacaaggc caggcggtgg atctgaacag cctgaaacgc tcccccaagc
     1141 aacaacaggc gctggcggcg ttacggcaag gcaaaatctg gcgcgaccag gtcgccacgc
     1201 tcgaatttaa tgatgccgcg ttgcaggcgc tacgcaaaaa aggtctgtgt gatttagcaa
     1261 gtgaaacacc agagtttagc gactggcgaa cgaactatgc cgtttctggt gagcggttgc
     1321 gattgaatac cgaacaggcc accgccgttg gcgcaattca tagcgcggca gatacttttt
     1381 ctgcctggct gctggcgggc gttaccggtt ccggtaaaac ggaggtttat ctcagtgtac
     1441 tggaaaacgt gctcgctcag ggcaaacagg cgctggtgat ggtgccggaa atcggcctga
     1501 caccgcaaac tatcgcccgt tttcgtgaac gttttaatgc ccccgtggaa gttctgcatt
     1561 ccggcctgaa cgacagcgag cggctttctg cgtggttgaa agcgaaaaat ggcgaggcgg
     1621 cgattgtgat cggcacccgc tccgcgctgt ttacgccgtt taaaaatctc ggcgtgattg
     1681 tcattgatga agagcacgac agctcctaca agcagcagga aggctggcgc tatcatgccc
     1741 gcgacctggc ggtgtatcgt gcgcacagcg agcaaatccc gattattctt ggctccgcaa
     1801 cgcccgcgct ggaaacgtta tgcaacgtcc agcagaaaaa ataccgcctg ctgcgcctga
     1861 cccgtcgggc agggaatgct tgtccggcaa ttcaacatgt gctggattta aaaggtcaga
     1921 aggtgcaggc aggtctggct ccggcgttaa tcacccgtat gcgccagcat ttacaggcca
     1981 acaatcaggt tatcctcttt cttaatcgtc ggggttttgc gcctgcgctg ctgtgccacg
     2041 actgcggctg gatagccgaa tgcccacgtt gcgatcacta ctacacgctg catcaggcgc
     2101 aacagcattt gcgctgtcac cactgtgaca gtcagcgtcc ggtgccgcgc cagtgccctt
     2161 cctgcggttc cacgcacctg gtccccgtgg ggctgggcac cgaacagctt gaacagacgc
     2221 ttgcgccgat gttccccggc gtgcccattt cacgtatcga ccgcgatacc accagccgca
     2281 aaggggcact ggaacagcaa ctggcggaag tgcatcgcgg cggcgcgagg attttgatcg
     2341 gcacacaaat gctggcgaaa ggtcaccact ttccggatgt gacgctggtg gcgttactgg
     2401 atgtggacgg cgcgctgttt tctgccgatt ttcgctcggc agaacgtttc gcccagcttt
     2461 acacccaggt cgccggtcgt gccgggcgtg cgggtaaaca gggcgaagtg gtgctgcaaa
     2521 cgcaccatcc ggaacatcct ctgttgcaaa cgttgctcta taaaggctac gacgcctttg
     2581 ccgaacaggc gctggctgag cgacgcatga tgcagctgcc gccgtggacc agccatgtga
     2641 ttgtgcgtgc ggaagatcat aacaatcagc acgcgccgct gttcctgcaa caactgcgta
     2701 atctgatcct ttccagcccg ctggcagacg acaaactgtg gattctcggc ccggttccgg
     2761 ctctggcacc taagcgtggt ggccgctggc gctggcagat cttgttgcag cacccttccc
     2821 gcgtgcgctt gcaacacatc attagtggta cgctggcgct tattaataca ataccggatt
     2881 cccgcaaggt gaaatgggtg ttggatgtcg atcccatcga aggctaatgc gcaaacgttg
     2941 cgaggcggat cgaaaaattc aatattcatc acacttttca tgaaaattct gtaaccgttt
     3001 tcacgcgcta tctgctaaaa atgttgccga tgtgaagtaa acatggatgt agtacgcctg
     3061 acgtaccagg cgaggagtga gtgtgaaagc gaagaagcag gaaactgccg cgaccatgaa
     3121 agacgttgcc ctcaaggcaa aagtctctac agcgactgtc tcccgagcat taatgaatcc
     3181 cgataaagtc tcccaggcca cccgtaatcg ggttgaaaaa gcggcccggg aagtgggtta
     3241 tttaccgcag cctatggggc gcaacgtcaa gcgtaatgaa tcccgcacca ttctggtgat
     3301 tgtcccggat atctgcgatc ccttctttag cgaaattatt cgcggtatcg aagttacggc
     3361 ggcaaatcac ggatatctgg tgctgattgg cgactgtgcg catcaaaatc agcaggaaaa
     3421 aacctttatc gatttgatca tcaccaagca aattgatggc atgttgctgc tgggttcaag
     3481 gctgccgttt gatgccagca ttgaggaaca gcgtaatctg ccgccgatgg tgatggcgaa
     3541 cgaatttgca ccggaactgg agctgcctac cgttcatatc gacaatctga ccgccgcatt
     3601 tgatgcagta aattatttat atgagcaagg gcataaacgg attggctgta tagccggtcc
     3661 cgaagagatg ccactgtgtc actaccgcct gcaaggctat gttcaggcgc tgcgtcgctg
     3721 cggaattatg gttgatccac aatacatcgc ccgtggcgac ttcaccttcg aagccggaag
     3781 caaagcaatg caacagctgc ttgatcttcc acaaccgcct actgcagtct tctgccatag
     3841 cgatgtgatg gcgctcggcg cactttctca ggcaaaacgc caggggctga aagtcccgga
     3901 agacctttcc ataatcggtt ttgataacat cgacctgacg caattttgtg atccgccgct
     3961 gacaaccatc gcgcagccgc gttacgaaat cggtcgggaa gctatgctgt tattgcttga
     4021 tcaaatgcag gggcaacacg ttggcagtgg ctctcgttta atggactgcg aacttatcat
     4081 ccggggatca acacgcgcgt taccttaaag taaaccttta agactgccgt gactggtcaa
     4141 aggcccgccg cttaagtaac atggcgggct gacgaacgaa taaatacagc gaaacgatag
     4201 tggcacaacg agattatgta cgccgcagcc aaccggcacc ttcgcggcga aaaaagagca
     4261 cctcacggaa aaagcaacga aatctgcctg cggtttctcc cgctatggtc gctattgctg
     4321 ccgccgttct tgtgaccttt atcggtggtc tgtacttcat tacgcatcac aagaaagaag
     4381 agtccgagac gctgcaaagc cagaaagtga ccggaaacgg actaccacca aaaccagaag
     4441 aacgctggcg ctacattaaa gagctggaaa gtcgccagcc gggtgtgcgt gcgcccacag
     4501 aaccttctgc cggtggtgaa gtgaaaacgc cggagcaact gacaccagaa caacgtcagc
     4561 ttcttgaaca aatgcaggct gatatgcgcc agcagccaac gcagctggtt gaggttccgt
     4621 ggaatgaaca gacgccggaa cagcgtcagc aaacgctaca acgccagcgt caggcgcagc
     4681 aattagcgga gcagcaacgc ctggctcagc agtcgcgtac gactgaacaa agctggcagc
     4741 agcagacgcg tacgtcgcaa gccgcgccgg tacaggcgca accgcgccag tctaaaccag
     4801 cttctactca gcagccgtac caggatctgc tgcaaacccc tgcgcacacg accgcgcagt
     4861 cgaagccgca gcaagctgca ccggttgctc gtgccgctga cgcgccaaaa ccgacggcgg
     4921 agaaaaaaga cgaacgccgc tggatggtgc agtgcggttc gttcagaggc gcggaacagg
     4981 cagagacggt acgtgctcag ctggcgttcg aaggatttga ctcgaaaatc accaccaaca
     5041 atggctggaa tcgtgtggtc attggcccgg tgaaaggcaa agagaacgca gacagcaccc
     5101 tcaatcggtt gaagatggcg ggtcatacaa actgcattcg gctcgccgcc gggggttgaa
     5161 accctcaaaa tcccccccat ctataattgc attatgcctc gtacttttgt acggggtttg
     5221 tactctgtat tcgtaaccaa ggggtcagct cgtgacaact atagtaagcg tacgccgtaa
     5281 cggccatgtg gtcatcgctg gtgatggtca ggccacgttg ggcaataccg taatgaaagg
     5341 caacgtgaaa aaggtccgcc gtctgtacaa cgacaaagtc atcgcgggct ttgcgggcgg
     5401 tactgcggat gcttttacgc tgttcgaact gtttgaacgt aaactggaaa tgcatcaggg
     5461 ccatctggtt aaagccgccg ttgagctggc aaaagactgg cgtaccgatc gcatgctgcg
     5521 caaacttgaa gcactgctgg cagtcgcgga tgaaactgca tcgcttatca tcaccggtaa
     5581 cggtgacgtg gtgcagccag aaaacgatct tattgctatc ggctccggcg gcccttacgc
     5641 ccaggctgcg gcgcgcgcgc tgttagaaaa cactgaactt agcgcccgtg aaattgctga
     5701 aaaggcgttg gatattgcag gcgacatttg catctatacc aaccatttcc acaccatcga
     5761 agaattaagc tacaaagcgt aaggatctcc catgtctgaa atgaccccac gcgaaatcgt
     5821 cagcgaactg gataagcaca tcatcggcca ggacaacgcc aagcgttctg tggcgattgc
     5881 tctgcgtaac cgctggcgtc gcatgcagct caacgaagag ctgcgccatg aagtgacccc
     5941 gaaaaatatc ctgatgatcg gcccgaccgg tgtcggtaaa actgaaatcg cccgtcgtct
     6001 ggctaagctg gcgaatgcgc cgttcatcaa agttgaagcg accaaattca ccgaagtggg
     6061 ctatgtcggt aaggaagtgg attctattat tcgcgatctg accgatgccg ccgtgaaaat
     6121 ggtacgcgtc caggctatcg agaaaaaccg ttatcgcgct gaagaactgg cagaagaacg
     6181 tattctcgac gtgctgatcc cacctgctaa aaacaactgg ggacagaccg aacagcagca
     6241 ggaaccgtcc gctgctcgtc aggcattccg caaaaaactg cgtgaaggcc agcttgatga
     6301 caaagaaatc gagatcgatc ttgccgcagc accgatgggc gttgaaatta tggctcctcc
     6361 aggcatggaa gagatgacca gccagctgca gtccatgttc cagaacctgg gcggccagaa
     6421 gcaaaaagcg cgtaagctga aaatcaaaga cgccatgaag ctgctgattg aagaagaagc
     6481 ggcgaaactg gtgaacccgg aagagctgaa gcaagacgct atcgacgctg ttgagcagca
     6541 cgggatcgtg tttatcgacg aaatcgacaa aatctgtaag cgcggcgagt cttccggtcc
     6601 ggatgtttct cgtgaaggcg ttcagcgtga cctgctgccg ctggtagaag gttgcaccgt
     6661 ttccaccaaa cacgggatgg tcaaaactga ccacattctg tttatcgctt ctggcgcgtt
     6721 ccagattgcg aaaccgtctg acctaatccc ggaactgcaa ggtcgtctgc caatccgcgt
     6781 tgaactgcag gcactgacca ccagcgactt cgagcgtatt ctgaccgagc cgaatgcctc
     6841 tatcaccgtg cagtacaaag cactgatggc gactgaaggc gtaaatatcg agtttaccga
     6901 ctccggtatt aaacgcatcg cggaagcggc atggcaggtg aacgaatcta ccgaaaacat
     6961 cggtgctcgt cgtttacaca ctgttctgga gcgtttaatg gaagagattt cctacgacgc
     7021 cagcgattta agcggtcaaa atatcactat tgacgcagat tatgtgagca aacatctgga
     7081 tgcgttggtg gcagatgaag atctgagccg ttttatccta taatcgcgtt caatcatttt
     7141 catcattgtt tgatggggct gaaaggcccc atttttattg gcgcgtatta tgactgaaca
     7201 acaaattagc cgaactcagg cgtggctgga aagtttacga cctaaaaccc tccccctcgc
     7261 ctttgctgca attatcgtcg ggacggcgct ggcatggtgg caaggtcatt ttgatccgct
     7321 ggtcgccctg ctggcactga ttaccgccgg gctattacag atcctttcta acctcgccaa
     7381 tgattacggc gatgcggtaa aaggcagcga tagacctgac cgcattgggc cgctacgcgg
     7441 catgcaaaaa ggggtcatta cccagcaaga gatgaaacgg gcgctcatta ttaccgtcgt
     7501 gctcatctgt ctctccgggc tggcactggt tgcagtggcg tgccatacgc tggctgattt
     7561 tgtcggtttc ctgattcttg gcgggttgtc gatcattgcc gctatcacct acaccgtggg
     7621 caatcgtcct tatggttata tcggtctggg ggatatttcc gtactggttt tctttggctg
     7681 gttaagcgtc atggggagct ggtatttaca ggctcataca ttgattccgg cactgatcct
     7741 tccggcgacc gcatgcggcc tgctggcaac ggcagtactg aacattaata acctgcgtga
     7801 tatcaatagc gaccgagaaa acggcaaaaa cacgctggtg gtgcgcttag gtgaagtgaa
     7861 cgcgcgtcgt tatcatgcct gcctgctgat gggctctctg gtatgtctgg cgctgtttaa
     7921 tctcttttcg ctgcatagcc tgtggggctg gctgttcctg ctggcggcac cgttgctggt
     7981 gaagcaagcc cgttatgtga tgcgggaaat ggacccggtg gcgatgcgac cgatgctgga
     8041 acgcactgtc aagggggcgt tactgactaa cctgctgttt attttaggga tattcctaag
     8101 ccagtgggca gcataactga caaatatcaa ttaacaattg atgattttgc caacagccca
     8161 catagcgcga tatactgaaa attctcgcag caactgaatg ttaagcctat gaaatacgat
     8221 acttccgagc tttgtgacat ctatcaagaa gatgttaacg tcgtggaacc gctgttctcc
     8281 aactttggcg gacgggcgtc gtttggcgga caaataatca cggtaaaatg tttcgaggac
     8341 aacgggttgc tgtacgatct gctcgaacag aatggccgtg gtcgtgttct tgtcgtcgat
     8401 ggcggtggtt ctgttcgtcg cgcactggtc gatgctgaac tggcgcgtct ggcagtacaa
     8461 aatgaatggg aaggtctggt tatttacggc gcggtgcgtc aggtagatga cctggaagag
     8521 ttggatatcg gcatccaggc gatggcggca attccggttg gtgccgctgg cgaaggcatt
     8581 ggcgaaagcg atgtccgcgt caattttggc ggtgtaacct tcttctccgg cgaccatctt
     8641 tatgccgaca ataccgggat tattctttca gaagatccgc tggatattga atgataagaa
     8701 aggcaccgca aggtgccttt tttctgcgtt acctgttggc ctacacagta aagaaattac
     8761 gcgaaagatg aagcgtaatc agacctcttc catgcgaccc agcagggcct gcagacgttc
     8821 ctgccagccg ttctgctgtt ctttcagatg gttgttctca cgctccagct cttcgcgctg
     8881 atgctgggca ttttgaactt cctgcgacag tgagttgttt ttttctttca gctcttcgat
     8941 ttccatctgc aacagagtga tggtatcaat cgcctgctgt acttttgctt ccagtttctc
     9001 aaacacttct aatgacattg tcatacctct cctgaattgc aaggcgttga tggataaaaa
     9061 atcctcgtcc cgattaccgg tgacgcctta ataaatacga gcgcacttta gttagctccg
     9121 attgtatgaa gccgcgccat cgctgtccag cggcacgcct tgcagattac ggtttgccac
     9181 acttttcatc cttctcctgg tgacataatc cacatcaatc gaaaatgtta ataaatttgt
     9241 tgcgcgaatg atctaacaaa catgcatcat gtacaatcag atggaataaa tggcgcgata
     9301 acgctcattt tatgacgaag cacacacatt ttaagttcga tatttctcgt ttttgctcgt
     9361 taacgataag ttaacagcat gcctacaagc atcgtggagg tccgtgactt tcacgcatac
     9421 aacaaacatt aactcttcag gatccgatta tgagtcaaac atcaaccttg aaaggccagt
     9481 gcattgctga attcctcggt accgggttgt tgattttttt cggtgtgggt tgcgttgcag
     9541 cactaaaagt cgctggtgcg tcttttggtc agtgggaaat cagtgtcatt tggggactgg
     9601 gggtggcaat ggctatctac ctgaccgcag gggtttccgg cgcgcatctt aatcccgctg
     9661 ttaccattgc attgtggctg tttgcctgtt tcgacaagcg caaagttatt ccttttatcg
     9721 tttcacaagt tgccggcgct ttctgtgctg cggctttagt ttacgggctt tactacaatt
     9781 tatttttcga cttcgagcag actcatcaca ttgttcgcgg cagcgttgaa agtgttgatc
     9841 tggcaggcac tttctctact taccctaatc ctcatatcaa ttttgtgcag gctttcgcag
     9901 ttgagatggt gattaccgct attctgatgg ggctgatcct ggcgttaacg gacgatggca
     9961 acggtgtacc acgcggccct ttggctccct tgctgattgg tctactgatt gcggtcattg
    10021 gcgcatctat gggcccattg acgggttttg ccatgaaccc agcgcgtgac ttcggtccga
    10081 aagtctttgc ctggctggcg ggctggggca atgtcgcctt taccggcggc agagacattc
    10141 cttacttcct ggtgccgctt tttggcccta tcgttggcgc gattgtaggt gcatttgcct
    10201 accgcaaact gattggtcgc catttgcctt gcgatatctg tgttgtgaaa gaaaaggaaa
    10261 ccacaactcc ttcagaacaa aaagcttcgc tgtaatatga ctacgggaca attaaacatg
    10321 actgaaaaaa aatatatcgt tgcgctcgac cagggcacca ccagctcccg cgcggtcgta
    10381 atggatcacg atgccaatat cattagcgtg tcgcagcgcg aatttgagca aatctaccca
    10441 aaaccaggtt gggtagaaca cgacccaatg gaaatctggg ccacccaaag ctccacgctg
    10501 gtagaagtgc tggcgaaagc cgatatcagt tccgatcaaa ttgcagctat cggtattacg
    10561 aaccagcgtg aaaccactat tgtctgggaa aaagaaaccg gcaagcctat ctataacgcc
    10621 attgtctggc agtgccgtcg taccgcagaa atctgcgagc atttaaaacg tgacggttta
    10681 gaagattata tccgcagcaa taccggtctg gtgattgacc cgtacttttc tggcaccaaa
    10741 gtgaagtgga tcctcgacca tgtggaaggc tctcgcgagc gtgcacgtcg tggtgaattg
    10801 ctgtttggta cggttgatac gtggcttatc tggaaaatga ctcagggccg tgtccatgtg
    10861 accgattaca ccaacgcctc tcgtaccatg ttgttcaaca tccataccct ggactgggac
    10921 gacaaaatgc tggaagtgct ggatattccg cgcgagatgc tgccagaagt gcgtcgttct
    10981 tccgaagtat acggtcagac taacattggc ggcaaaggcg gcacgcgtat tccaatctcc
    11041 gggatcgccg gtgaccagca ggccgcgctg tttggtcagt tgtgcgtgaa agaagggatg
    11101 gcgaagaaca cctatggcac tggctgcttt atgctgatga acactggcga gaaagcggtg
    11161 aaatcagaaa acggcctgct gaccaccatc gcctgcggcc cgactggcga agtgaactat
    11221 gcgttggaag gtgcggtgtt tatggcaggc gcatccattc agtggctgcg cgatgagatg
    11281 aagttgatta acgacgccta cgattccgaa tatttcgcca ccaaagtgca aaacaccaat
    11341 ggtgtgtatg tggttccggc atttaccggg ctgggtgcgc cgtactggga cccgtatgcg
    11401 cgcggggcga ttttcggtct aactcgtggg gtgaacgcta accacattat acgcgcgacg
    11461 ctggagtcta ttgcttatca gacgcgtgac gtgctggaag cgatgcaggc cgactctggt
    11521 atccgtctgc acgccctgcg cgtggatggt ggcgcagtag caaacaattt cctgatgcag
    11581 ttccagtccg atattctcgg cacccgcgtt gagcgcccgg aagtgcgcga agtcaccgca
    11641 ttgggtgcgg cctatctcgc aggcctggcg gttggcttct ggcagaacct cgacgagctg
    11701 caagagaaag cggtgattga gcgcgagttc cgtccaggca tcgaaaccac tgagcgtaat
    11761 taccgttacg caggctggaa aaaagcggtc aaacgcgcga tggcgtggga agaacacgac
    11821 gagtaatgta aatgccggat gaagcgttta tgccgcatcc ggtagtcctg aaacgtgcgg
    11881 gggcaacccc gcacacatca ataatcccac ccttcccctg tgctacactt cgcgccattc
    11941 cttactgctt agagtttgct atgagacgag aacttgccat cgaattttcc cgcgtcaccg
    12001 aatctgcggc gctggctggc tacaaatggt taggacgcgg cgataaaaac accgcggacg
    12061 gcgcggcggt aaacgccatg cgtattatgc tcaaccaggt caacattgac ggcaccatcg
    12121 tcattggtga aggtgaaatc gacgaagcac cgatgctcta cattggtgaa aaagtcggta
    12181 ctggtcgcgg cgacgcggta gatattgctg ttgatccgat tgaaggcacg cgcatgacgg
    12241 cgatgggcca ggctaacgcg ctggcggtgc tggcagtagg cgataaaggc tgcttcctca
    12301 atgcgccgga tatgtatatg gagaagctga ttgtcgggcc gggagccaaa ggcaccattg
    12361 atctgaacct gccgctggcg gataacctgc gcaatgtagc ggcggtgctc ggcaaaccgt
    12421 tgagcgaact gacggtaacg attctagcta aaccacgcca cgatgccgtt atcgctgaaa
    12481 tgcagcaact cggcgtacgc gtatttgcta ttccggatgg cgacgttgcg gcctcaattc
    12541 tcacctgtat gccagacagc gaagttgacg tgctgtacgg tattggtggc gcgccggaag
    12601 gcgtagtttc tgcggcggtg atccgcgcat tagatggcga catgaacggt cgtctgctgg
    12661 cgcgtcatga cgtcaaaggc gacaacgaag agaatcgtcg cattggcgag caggagctgg
    12721 cacgctgcaa agcgatgggc atcgaagccg gtaaagtatt gcgcctgggc gatatggcgc
    12781 gcagcgataa cgtcatcttc tctgccaccg gtattaccaa aggcgatctg ctggaaggca
    12841 ttagccgcaa aggcaatatc gcgactaccg aaacgctgct gatccgcggc aagtcacgca
    12901 ccattcgccg cattcagtcc atccactatc tggatcgcaa agacccggaa atgcaggtgc
    12961 acatcctctg attgatttga tcgattgagc cttccagtcc ttcgggactg gaattttttt
    13021 gttcggagaa cgaagataag gcaagtcaat caaaacagga gaaaaacatg gctgattggg
    13081 taacaggcaa agtcactaaa gtgcagaact ggaccgacgc cctgtttagt ctcaccgttc
    13141 acgcccccgt gcttccgttt accgccgggc aattcaccaa gcttggcctt gaaatcgacg
    13201 gcgaacgcgt ccagcgcgcc tactcctatg ttaactcgcc cgataatccc gatctggagt
    13261 tttacctggt caccgtcccc gatggcaaat taagcccacg attggcggca ctgaaaccag
    13321 gcgatgaagt gcaggtggtt agcgaagcag ctggcttctt tgttctggat gaagtaccag
    13381 attgcgaaac gctatggatg ctggcaaccg gtacagcgat tggcccttat ttatcgattc
    13441 tgcaactagg caaagattta gatcgcttca aaaatctggt cctggttcac gccgcacgtt
    13501 atgccgccga cttaagctat ttgccactga tgcaagaact ggaaaaacgc tacgaaggca
    13561 aactgcgcat tcagacggtg gtcagtcggg aaacggcagc ggggtcgctc accggacgga
    13621 taccggcatt aattgaaagt ggggaactgg aaagcgcgat tggcctgccg atgaataaag
    13681 aaaccagcca tgtgatgctg tgcggcaatc cacagatggt gcgcgataca caacagttgc
    13741 tgaaagagac ccggcagatg acgaaacatt tacgtcgccg accgggccat atgacagcgg
    13801 agcattactg gtaagcggtt acttatcgat aaacggcacg atgagcaaat ccgcactcat
    13861 cttattgatc atcccgcgat atgctggcat caaacggttg ataaatgagt gatgatgacc
    13921 acagacaagg aggtcgcact gctctttttg cataatttcc agcagtgttt ccggcatttc
    13981 tccgcgttca atacgcagtt ttgtcttcgg ccattgaata tttttcgtca gtttatacag
    14041 cttgttatcc gacttattct tcaacaattg aagaatatct tctgttgcag ggaagtagat
    14101 acccggatac aactcgctta agccatcatc aatatgaatt aacgtcaggt gagcgtcatt
    14161 atgtctggcg agctccagag ctttattcac cagtaaggca tcttcttcat tcccggaaat
    14221 tgccacgcca atgtgtttat aagccatgtt taactccttc taaagcaact ccatcaagct
    14281 aacgaacgca gtgataactc aaaaataatc atctcggcct ggcatgagaa agtgaaggcc
    14341 gcatcaagct caacttgccc ctccagatcc ttaatcttgt aggccacgct ggccccttca
    14401 tctgcagagg atgcagcggc ggcaagctct ttcagtttgg tcataaaaga ctctgcttct
    14461 tcgcgggtgg caaaaaagcg cgaaaactta ctggtacagt tgtcgttatc aatcaccgta
    14521 ccgatatcaa tcgcacatcc tttagtactg catttatcta cgacatcttt catagggcac
    14581 ctctgtatat tcgcctctct gtcgattacg acttaagaat aatgcgccgt taagggaaga
    14641 acaaacaagt aatgatctta tggattacca ttttttttga tattgcttaa ggtgaagagt
    14701 tcagaaggat tttccgggta ttgtcatttg catcgtggaa ataacaatcc cggaaaaaaa
    14761 tagcagtaag agatatatta aaggttaatt acttgattta ttgtcggctt tatacttcac
    14821 atcctgagta tctttaccat atttattttc gccttgtgtg ccaacaaacg caccaagatc
    14881 gataagcatc atcaccagaa tcaacgtcgg gacaaaacac cccaccgccc attgccagac
    14941 acccggtaaa atcgcccagt tacccgccag cagcatccac gccacaatca tcagaaatgc
    15001 ccatgcgccg gaacgcccgc gatcatgcaa gcgcttaaca gttactgccg ctgttggcca
    15061 aagcaagcac acaaggcaaa acgccgcggt ctgaatatcg agtaaatttt tacccgccag
    15121 tgagaaaagc accagcatgc ctgcgaacca caggcctatc caaatccaga aatcacggcg
    15181 tccaatacgc cctttaaatg agaataacca ttgctgtatg gtcatgtaag ttccttgatg
    15241 gttgtctttt ccaggattct acccttttga caagggcgac agatatcgtt ttaatcggag
    15301 ccagtcataa caaaaggtac tgtcaatgaa gccagggtgt acgctgtttt ttctcttatg
    15361 ttctgcatta accgttacaa caacggcgca tgcacaaaca ccagatacgg caacgaccgc
    15421 gccttatctg ctggctggag cccctacttt cgatctctcc atcagccagt ttcgagaaga
    15481 ctttaacagc cagaatccta gcctgccact gaacgaattt cgtgccatcg acagcagtcc
    15541 cgacaaagcc aatctcactc gtgctgcaag taaaattaac gagaacttgt atgcttctac
    15601 agcgctggag cgcggtacct taaaaatcaa aagcattcaa atgacctggc tacccatcca
    15661 ggggccagag caaaaagcag cgaaagcgaa agctcaggaa tacatggcag cagtgatccg
    15721 cacactcacc ccattaatga ccaaaacaca aagccagaaa aaactgcagt cgctgctaac
    15781 ggcggggaaa aacaaacgtt attacaccga gacagaaggt gcactgcgtt atgttgtcgc
    15841 ggacaacggc gaaaaggggc tgaccttcgc tgttgaaccg attaagctgg cgctatctga
    15901 atcgcttgaa ggtttgaata aatgacaaaa agcaaagcct ttgtgccgat gaatctctat
    15961 actgtttcac agacctgctg ccctgcgggg cggccatctt cctttattcg cttataagcg
    16021 tggagaatta aaatgcgaca tcctttagtg atgggtaact ggaaactgaa cggcagccgc
    16081 cacatggttc acgagctggt ttctaacctg cgtaaagagc tggcaggtgt tgctggctgt
    16141 gcggttgcaa tcgcaccacc ggaaatgtac atcgatatgg cgaagcgcga agctgaaggc
    16201 agccacatca tgctgggtgc gcaaaacgtg gacctgaacc tgtccggcgc attcaccggt
    16261 gaaacctctg ctgctatgct gaaagacatc ggcgcacagt acatcatcat cggccactct
    16321 gaacgtcgta cttaccacaa agagtctgac gaactgatcg cgaaaaaatt cgcggtgctg
    16381 aaagagcagg gtctgactcc ggttctgtgc atcggtgaaa ccgaagctga aaacgaagcg
    16441 ggcaaaactg aagaagtttg cgcacgtcag atcgacgcgg tactgaaaac tcagggtgct
    16501 gcggcattcg aaggtgcggt tatcgcttac gaacctgtat gggcaatcgg tactggcaaa
    16561 tctgcaactc cggctcaggc acaggctgtt cacaaattca tccgtgacca catcgctaaa
    16621 gttgacgcta acatcgctga acaagtgatc attcagtacg gcggctctgt aaacgcgtct
    16681 aacgctgcag aactgtttgc tcagccagac atcgacggcg cgctggttgg cggtgcgtct
    16741 ctgaaagctg acgctttcgc agtaatcgtt aaagctgcag aagcggctaa acaggcttaa
    16801 gtctgacagg tgccggattt catatccggc acttactttc cttaactctt cgccttaacg
    16861 caaaatctca cactgatgat cctgaatttc ctcggctgaa gcacggttaa gcgtcagtag
    16921 atttcgttgt gtcgccagca atacaaatga gttatcactc tgccgtacca tcgccagccc
    16981 gtagcttccc atatgttccc gcgcctcagg tacttcttct gccagcatca taaatgggct
    17041 gcgttgtacc agttcgcttt ccgttacccg acgcgccagg tattcatgcc cgcgcaaacc
    17101 acctggcagt ggcaaccagc ggctgctgat gttcgccaga ttgttatcca gttgttcgcg
    17161 cacatcagga cgaatacaag agatatgaat atgaaaatgg ttttgcgtac gcccggtgcg
    17221 ggagttgatc gccaaagaaa ccgcgcgatc gggaaccggc tggccgtact ttttgctcat
    17281 aaaatcacgc gcctgccagg ccaaccaaaa gaagttcggc gttgaaggat cggtcaacaa
    17341 aggactttca gtaccgttaa tacgatacgt tggcatcaac agatattgca gtgggccatt
    17401 aagatctttt aaaaccacgt atccggcatt gggtttgact tccgcacatg gcgaaggatt
    17461 ttgattttgc tgctgattgg gcaaacattc ctcaaggaca atcttacgta atgtatccga
    17521 ctcttcaccg gttaatttcc agtaaccaat accggcagcc acaacggcga taactatcat
    17581 caccaaaaaa agaagacccg cttttttcat ctttttttcc ctgtacctca aagagatgca
    17641 agggtaacgc aaaatcgtga caaataaaaa acccggtcgc gagaaaccac cgggtttgat
    17701 aattatcctg gggaaatcag cgtttgctga tctgatcgaa cgtaccgccg ttagcaaaat
    17761 gctctttttg cgctttcgtc cagccgccga actcttcatc aatggtgaat aacttcagct
    17821 ttggaaacgc attttcgtac tttttcgcta cttcagcgtc gcgcggacgg tagtagtttt
    17881 tcgcggcaat ttcctgacct tctggcgagt agagatattt caggtaggct tccgccacct
    17941 ctttggtgcc ttttttctcg accactttat cgaccaccga cacggttggc tctgcgagga
    18001 tagactcact cggcgtgacg atttcgaatt tatctttccc cagttcattc gctgccagca
    18061 gagcttcgtt ttcccaggcg atcagtacat cgccaatccc gcgctcgaca aaggtgttag
    18121 tggagccgcg cgcgccagaa tccagaactt cgacgttttt atacagtgcc cgaacaaaat
    18181 cctgtgcttt tgcctgatcg ttgttgttgt gatgcagcgc gtagccccag gcagccaggt
    18241 agttccagcg tgcgccacca gagcttttcg gattaggcgt gatcaccgaa acacccggtt
    18301 taatcagatc gttccagtca tggatctgct tcggattgcc cttacgcacc aggaaaacaa
    18361 tggtggaagt gtacggtgcg gagttatccg gcagacgttt gatccactct ttatcaatcc
    18421 gcccgcgttc cgcaattgcg tccacgtcat aggccagagc cagcgtgaca acatcagctt
    18481 caataccgtt gattaccgac gtcgcttgtt tacctgaacc accgtgtgac tgacgcatca
    18541 ccacgttatc accagtttgc tgtttccagt gggcgctgaa tgccttgttg tactgttcgt
    18601 acaattcgcg cgttggatca tatgaaacgt taagaagctg aatatccttt gccataacgc
    18661 tggttgccgc cagcaaaaat gttaacccta cgccccactt gttcatcgcc cgactctctt
    18721 atgttgtgtt gtgatgagca aagcgtgcca gaaggttaac caaacattaa agaataaaaa
    18781 aagattggct ataacttgcg ggtatatgtt gagggattaa aaaggcggaa gaaatccgcc
    18841 tcatattgct gacaaagtgc gctttgtcca tgccggatgc ggcgcgaacg ccttatccgg
    18901 cctacaaaag tttgcaaatt caataaattg cagaattcat gtaggcctga taagcgaagc
    18961 gcatcaggca tttttgcttc tgtcatcggt ttcaggctaa aggaatctgc ctttttccga
    19021 aatcattaat acagtttttt cgcgcagtcc agccagtcac ctttgaacgg acgcttcatg
    19081 ttttcgatag cgtcgatgat gtcgtggtga accagctgtt cgttctggat acctacacaa
    19141 cgaccgccgt aacctgccag cagcagatcg atagcgtaag cgcccatacg ggaagccaga
    19201 atacggtcgt aaggcaccgg agaaccaccg cgctggatgt ggcccagcac agttgcgcgg
    19261 gtttcacgac ctgtttcttt ctcgatgaaa tgcgccagtt cgtcaacatc acacatatgt
    19321 tcggtaatcg ccacgatcgc gtgtttttta cctttcgcga tacccgcttt gatttcgttt
    19381 accaggtctt cacggctgaa ttcaacttcc ggaaccacaa cgaattcaca gccaccggca
    19441 atggccgcag ccaacgtcag atcgccacaa taacggccca tcacttccac cacggaaata
    19501 cgctggtgag aagaagaggt gtcacgcaga cggtcgatcg cttctacaac ggtgctcagc
    19561 gcagtgaaga aaccgatagt gtagtcagtg cctttgatgt cgttgtcgat agtgcccggc
    19621 agaccgatgc acgggaagcc catttcggtc agacgcattg aacccatgta ggaaccgtca
    19681 ccgccgataa ccaccagcgc gtcgatacca cgttttttca ggttttcgat agccacggcg
    19741 cggatgttct cgtcgcggaa ttccgggaaa cgcgcagaac cgaggaacgt accgccacgg
    19801 ttgatcatgt cagacacgct gtaacggtct agctgtacca tacggtcttc atacagaccc
    19861 agatagccgt cataaatacc cattacttcc agaccttctg tcagcgcaga acgaacaacc
    19921 ccgcgaattg cggcgttcat gcctggcgca tcaccgccgc ttgtcaacac accgattttc
    19981 ttaatcatga ctacctctga actttggaat gcaaaatgaa atctgttgcc ggaagtcttc
    20041 ttgcacatcg aagtgatcca acgaatgtgc aaatagtata acaatcactt cctgctgaat
    20101 tgattcaggt caggccaaat ggcggtattt tatacacaaa atgcgggtct ggctctcttt
    20161 tatactgatt atgaaagcat agaccgttta ccctccctgg gtacgacgga acaggggtcc
    20221 tgatggataa ttacatccga tcccggaaaa cgccgtaaaa tagcctgctc tacctgatcc
    20281 gccaccatat gtgcctgaac caaaggcaga gagtcttcca tttccaaatg aatctgaata
    20341 aagcgggtcg gccctgactg ccgcgtgcga agatcgtgag cgccgctaac acccggccag
    20401 gaagtcacga tatcaataat ttcttgccgt tcctcatcag gcaatgcgcg atccagtaat
    20461 gactgtaccg cctcatatcc catgcgtaac gcgctatata aaatatagat gccgattccc
    20521 aatgcaaaca gagcatcggc gcgatgccag ccgtaccagg acaaccccag cgccagcaga
    20581 attgcgccgt tcatcataac atcagactgg taatgtagca tatcagcccg caccgcctgg
    20641 ctttgcgtcc ggcgcaccac ccaacgctga aacgagacaa ggataatcgt acaaattagc
    20701 gccacaattg tcacgataac cccgacgcct ggatctatca tcggtgttgg agatatcaga
    20761 tgttgaatac ccgtcaaaaa caggaatagt gccgaaccgg agataaacat actttgcgcc
    20821 agcgccgcga gggactctgc tttaccgtga ccaaacgagt gattatcgtc ggcaggttgc
    20881 agggaatatc gcaccaccag taaattcgtc aacgacgcgc cgatatccac cagcgaatcc
    20941 accagcgcgg cgagaatact caccgacccg gtataccacc atgcaaaaat tttaatcagc
    21001 aatagcaacg aagccatcgc cgtcgcagca atcgccgccc gactgaccag ccgtccataa
    21061 gattgattca taaatactcc cgctatcaac tgacgctagt ataacggaag caaatcatct
    21121 gcaatgcatt aagcagcagg caaattgagg ataaaaaaaa ccccacatca tgtgggggaa
    21181 gacagggatg gtgtctatgg caaggaaaac agggtttact actgggaacg tgagttgcta
    21241 ctactcaata gcttcaacga tgaacttttt tgccattgcg tcacgtcacg caactgctcc
    21301 attcgttgtt gatgtttctc gtttaaaacc gcttgctgct ccggcgttaa caggcgatac
    21361 atttggttgc ggacttttgc catctcaacc tgacgagcaa tttgctcatt cgccattttt
    21421 tctgcctgtg cgcgcacagc gttttcatca aaattttctg cggtgacaag gcgatgcatt
    21481 gtctccagtt cgctaacatt aacaggaggc tgttcgtgcc gggcctgttg cataagatct
    21541 cgcatctgct gacgctgatg ttcggttaaa cttatgccgt cgaacatatg gctctgcgta
    21601 ctgcgctgcg tactgcgctg cgtactgcgc tgcgtactgc gctgcgtaag ttcttcaccc
    21661 ggatgccagt tatcgcctga accgacttca gcagcgtggc ttaatgaact gactgccagc
    21721 gttgaagcca tgacggcagc ggtaactatg cgcatcattt gctcccaaaa tctttctgtc
    21781 gcgattcaac gatagagagt ttacgattca ggctgcaaac atgcgtcagg gggtgtaaaa
    21841 caacgtaaag tcatggatta gcgacgtctg atgacgtaat ttctgcctcg gaggtattta
    21901 aacaatgaat aaaatcctgt tagttgatga tgaccgagag ctgacttccc tattaaagga
    21961 gctgctcgag atggaaggct tcaacgtgat tgttgcccac gatggggaac aggcgcttga
    22021 tcttctggac gacagcattg atttactttt gcttgacgta atgatgccga agaaaaatgg
    22081 tatcgacaca ttaaaagcac ttcgccagac acaccagacg cctgtcatta tgttgacggc
    22141 gcgcggcagt gaacttgatc gcgttctcgg ccttgagctg ggcgcagatg actatctccc
    22201 gaaaccgttt aatgatcgtg agctggtggc acgtattcgc gcgatcctgc gccgttcgca
    22261 ctggagcgag caacagcaaa acaacgacaa cggttcaccg acactggaag ttgatgcctt
    22321 agtgctgaat ccaggccgtc aggaagccag cttcgacggg caaacgctgg agttaaccgg
    22381 tactgagttt accctgctct atttgctggc acagcatctg ggtcaggtgg tttcccgtga
    22441 acatttaagc caggaagtgt tgggcaaacg cctgacgcct ttcgaccgcg ctattgatat
    22501 gcacatttcc aacctgcgtc gtaaactgcc ggatcgtaaa gatggtcacc cgtggtttaa
    22561 aaccttgcgt ggtcgcggct atctgatggt ttctgcttca tgataggcag cttaaccgcg
    22621 cgcatcttcg ccatcttctg gctgacgctg gcgctggtgt tgatgttggt tttgatgtta
    22681 cccaagctcg attcacgcca gatgaccgag cttctggata gcgaacagcg tcaggggctg
    22741 atgattgagc agcatgtcga agcggagctg gcgaacgatc cgcccaacga tttaatgtgg
    22801 tggcggcgtc tgttccgggc gattgataag tgggcaccgc caggacagcg tttgttattg
    22861 gtgaccaccg aaggccgcgt gatcggcgct gaacgcagcg aaatgcagat cattcgtaac
    22921 tttattggtc aggccgataa cgccgatcat ccgcagaaga aaaagtatgg ccgcgtggaa
    22981 ctggtcggtc cgttctccgt gcgtgatggc gaagataatt accaacttta tctgattcgt
    23041 ccggccagca gttctcaatc cgatttcatt aacttactgt ttgaccgccc gctattactg
    23101 ctgattgtca ccatgttggt cagtacgccg ctgctgttgt ggttggcctg gagtctggca
    23161 aaaccggcgc gtaagctgaa aaacgctgcc gatgaagttg cccagggaaa cttacgccag
    23221 cacccggaac tggaagcggg gccacaggaa ttccttgccg caggtgccag ttttaaccag
    23281 atggtcaccg cgctggagcg catgatgacc tctcagcagc gtctgctttc tgatatctct
    23341 cacgagctgc gcaccccgct gacgcgtctg caactgggta cggcattact gcgccgtcgt
    23401 agcggtgaaa gcaaggaact ggagcgtatt gaaaccgaag cgcaacgtct ggacagcatg
    23461 atcaacgatc tgttggtgat gtcacgtaat cagcaaaaaa acgcgctggt tagcgaaacc
    23521 atcaaagcca accagttgtg gagtgaagtg ctggataacg cggcgttcga agccgagcaa
    23581 atgggcaagt cgttgacagt taacttcccg cctgggccgt ggccgctgta cggcaatccg
    23641 aacgccctgg aaagtgcgct ggaaaacatt gttcgtaatg ctctgcgtta ttcccatacg
    23701 aagattgaag tgggctttgc ggtagataaa gacggtatca ccattacggt ggacgacgat
    23761 ggtcctggcg ttagcccgga agatcgcgaa cagattttcc gtccgttcta tcggaccgat
    23821 gaagcacgcg atcgtgaatc tggcggtaca ggattggggc tggcgattgt tgaaaccgcc
    23881 attcagcagc atcgtggctg ggtgaaggca gaagacagcc cgctgggcgg tttacggctg
    23941 gtgatttggt tgccgctgta taagcggagt taaactccgc atttgtaggc aggataaggc
    24001 gtttacgccg catccggcat ttgagcagga tgcctgatgc gacgctgata gcgtcttatc
    24061 aggcctacac tccgggggtt attttcccca caaccggcgg gaaaaatctt cgatcttgcc
    24121 gctcagtcgg cgcttttgca tcgtccgcgt ccagcttttc gataacccgg cggcggagag
    24181 taaacggtga tactgatcgt catcaaacgg catatgccat gcgatggcgg cagcctcctg
    24241 cacggtcaca tcgctgacac gggaaaccaa ctccagcggc gcgtccgcag acactttccc
    24301 tggcgctatc acgctataca accaccccac cttacctgtg ttttgcatca actgcgctat
    24361 atcgctgata tcaaaatggt aattgagttt atagcaaggc gagcgcggct ggctgacctg
    24421 aattaatgcc tctccccagc ggaaaatatc gcccatataa acattacttt ccgtcaggcc
    24481 gtcggttgag aggttttcac caaacgcagg cgcaacaaac aactccgcct gttccggaaa
    24541 ttcccgcgcc cagtagagat aatgctcacg aggataatga cacagcgctc tgtctggccc
    24601 accatgaacc tttttctccg cctgctcgtc accttccagc cccagctccg tcagcatcaa
    24661 ctcaccatca acctggattt tggcaattgc gctgggtttg ccttcgggat aagcctgaat
    24721 cttgcctgtg tatacatcaa ccggatatcg catctgttcc ctctccacga gaaatttccc
    24781 cattagacca tatttatttt atgtcaggcg tgctgttttc gcggtaagta tccaggcagg
    24841 aactcaatag caaaccggac gaacgttagt tcatccggct gtggggatta aggaagcagg
    24901 tttatttcac cgtacctaaa atttcgattt tcgctgctct ggctttgact ttacgtgacc
    24961 agatagaggt aagaattggc accagaatcg aagtcacaat gaccgccgtc gctaccagcg
    25021 aagttgctgc cggagccatc ggtttaaacg caggcaccat ttctgcaatc agcataggtg
    25081 tcgctaccgc ggcccctgcg gaactggaag cggcaattcc ggcagtgcca tcgccaccgc
    25141 caatcaattt atcggcgata atcaacggaa taccggtcac gataattact gccacaccca
    25201 acaggatccc cagcaaacca gtctgggcaa tcacagtcaa atcaatggta ttgcccagcg
    25261 cgaaggcaaa gaatggaatc agcgtttgca ccgctttgct gaaaaattct cgtaattcag
    25321 ggtcaaggtt ccccagggca aagcccacca ggaacggtaa tactgcgccg acgaaaacat
    25381 gcggttcaaa cgaggcaatc cctgcagtgc ccagaataat catcgtcatg agcggcccgg
    25441 actccaacga catcaacaca aatgccccag cttcttcttt tgtgccgtac tgctgcatga
    25501 tggaagcgta aagtccgcca ttggtcatat ccatcgccgc caccagcgcc agcgttgaaa
    25561 gtccggcaaa gaatccaact tcaacaccat gttccggaat aatgcgcgag gcaattgccg
    25621 caaccaccca cgcgacggca atttttgtta ccaccagcgt accggattta cgcaataccg
    25681 ttcccgtcgc gcttaatttt attgacgccc ccatgcaaaa aaaccacacc gccagaatgg
    25741 acaccgtacc ggtaatcatc ccgttggtga atgatccaaa atatttcccc gcccccggcg
    25801 agaaggtgtg gcacagtgcg ccaaggaata gcgggacgag catcatcccc ccccgggatt
    25861 ttctcaatcg agcgttttat ctgcatttcc atcacctgtc ggcatatttt taagggctga
    25921 tgcgtaattc ctcaatggag catcagtgtt gatggaaacg aagatagaca ttcatttaat
    25981 caataaaagt gatcaaaacc acatcaattg aaacgctgtt ttatttttat cggatcattg
    26041 tttttatttt gaaattgatc acaaaaaaac caccgccgtt ggcgatggtt cattttttaa
    26101 gctgatatgc ggcgtaaacg cctcattgca gcaggcggca aatgattatt ttttcgccgc
    26161 gaaacgtgcc gctgcttcat cccagttcac cacgttccag aactctttga tgtagtccgg
    26221 acggcggttc tggaatttca ggtagtaagc atgttcccac acatccaggc ccagaatcgg
    26281 gaagccggaa gcgccagaaa tagcttcacc catcagcggg gaatcctggt tagcagtaga
    26341 aaccaccgcc agtttatcgc ctttcagcac cagccatgcc cagccggaac caaagcggga
    26401 agctgccgct ttctcaaatt ctgctttgaa gttatcaacg gagccgaagt cacgttcgat
    26461 agccgctttc aggtcaccct gtaaggtggt gccttttttc agacctttcc agaacaggct
    26521 gtggttagcg tgaccgccag cgttgttgcg cagtacggtt ttcttgtctg ctggcagctg
    26581 gtccagtttg gtgatcagct cttcaaccgg caggttggca aattctggca ggctttccag
    26641 cgccgcgttg gcgttgttga cgtaggtctg atggtgtttg gtgtggtgga tttccatggt
    26701 ctgcttatcg aagtgcggtt ccagggcatc gtaagcatac ggcagggatg gcagggtata
    26761 gctcatattc atctccagta ttgtcgggcg gccgattgtt aatgcgcgta agcagttggt
    26821 tcattatagt taattaaatg atattgaaaa tgattatcaa tgccgtactt ttcgtaaggg
    26881 tatggttttg caggaaaatt cccgagatgt gaagcaaatc acccacttaa tgccgtgatt
    26941 gccagtaaat cgacaacggc ggcaacaggc gaaaggttaa tcgacagcac gatttttaca
    27001 ctcatctcgt cggagatgtg acgcgacgaa aaatgatgag gataagaaga tgagtaacgc
    27061 gattacgatg gggatatttt ggcatttgat cggcgcggcc agtgcagcct gtttttacgc
    27121 tccgttcaaa aaagtaaaaa aatggtcatg ggaaaccatg tggtcagttg gtgggattgt
    27181 ttcgtggatt attctgccgt gggccatcag cgccctgtta ctgccgaatt tctgggcgta
    27241 ttacagctcg tttagtctct ctacgctgct gcctgttttt ctgttcggcg ctatgtgggg
    27301 gatcggtaat atcaactacg gcctgaccat gcgttatctc ggcatgtcga tgggaattgg
    27361 catcgccatt ggcattacgt tgattgtcgg tacgctgatg acgccaatta tcaacggcaa
    27421 tttcgatgtt ctgcttaata ccgaaggcgg acgcatgacg ttgctcggcg ttctggtggc
    27481 gctgattggt gtagggattg tgactcgcgc cgggcagttg aaagagcgca aaatgggcat
    27541 taaagccgaa gagttcaacc tgaaaaaagg gctggtgctg gcggtgatgt gcggcatttt
    27601 ctctgccggt atgtcttttg cgatgaacgc cgcaaaaccg atgcatgaag ccgctgccgc
    27661 actgggcgtc gatccactgt atgtcgctct gccaagctat gtcatcatca tgggcggcgg
    27721 cgcgatcatc aacctcggtt tctgcttcat tcgtctggca aaagtgaagg atttgtcgct
    27781 aaaagccgac ttctcgctgg caaaaccgct gatcattcac aatgtgttac tctcagcact
    27841 gggcgggttg atgtggtatc tgcaattctt tttctatgcc tggggccacg cccgcattcc
    27901 ggcgcagtat gactacatca gttggatgct gcatatgagt ttctatgtat tgtgcggcgg
    27961 tatcgtcggg ctggtgctga aagagtggaa caatgcaggt cgccgtccgg taacggtgtt
    28021 gagcctcggt tgtgtggtga ttattgtcgc cgccaacatc gtcggcatcg gcatggcgaa
    28081 ttaatctttc tgcgaattga gatgacgcca ctggctgggc gtcatcccgg tttcccgggt
    28141 aaacaccacc gaaaaatagt tactatcttc aaagccacat tcggtcgaaa tatcactgat
    28201 taacaggcgg ctatgctgga gaagatattg cgcatgacac actctgacct gccgcagata
    28261 ttgattgatg gtcattccag tctgctggcg aaattgctga cgcaaaacgc gctcactgca
    28321 cgatgcctca tcacaaaatt tatccagcgc aaagggactt ttcaggctag ccgccagccg
    28381 ggtaatcagc ttatccagca acgtttcgct ggatgttggc ggcaacgaat cactggtgta
    28441 acgatggcga ttcagcaaca tcaccaactg cccgaacagc aactcagcca tttcgttagc
    28501 aaacgacaca tgctgactac tttcatgctc aagctgaccg ataacctgcc gcgcctgcgc
    28561 catccccacg ctacctaagc gccagtgtgg ttgccctgcg ctggcgctaa atcccggaat
    28621 cgctccctgc cagtcaagat tcagcttcag ccgctccggg caataaataa tattctgcaa
    28681 aaccagatcg ttaacggaag cgtaggagtg tttatcgtca gcatgaatgt aaaagagatc
    28741 gccacgggta atgcgataag ggcgatcgtt gagtacatgc agaccattac cgcgccagac
    28801 aatcaccagc tcacaaaaat catgtgtatg ttcggcaaaa acatcttgcg gataacggtc
    28861 agccacagcg actgcctgct ggtcgctggc aaaaaaatca tctttgagaa gttttaactg
    28921 atgcgccacc gtggctacct cggccagaga acgaagttga ttattcgcaa tatggcgtac
    28981 aaatacgttg agaagattcg cgttattgca gaaagccatc ccgtccctgg cgaatatcac
    29041 gcggtgacca gttaaactct cggcgaaaaa gcgtcgaaaa gtggttactg tcgctgaatc
    29101 cgcagcgata ggcgatgtca gtaacgctgg cctcgctgtg acgtagcaga tgtcgggcct
    29161 tcatcagtcg caggcggttc aggtaacgct gaggcgtcag tcccgtttgc tgcttaagct
    29221 gccgatgtag cgtacgcagt gaaagagaaa attgatccgc cacggcatcc caattcacct
    29281 catcggcaaa atggtcctcc agccaggcca gaagcaagtt gagacgtgat gcgctgtttt
    29341 ccaggttctc ctgcaaactg cttttacgca gcaagagcag taattgcata aacaagatct
    29401 cgcgactggc ggtcgagggt aaatcatttt ccccttcctg ctgttccatc tgtgcaacca
    29461 gctgtcgcac ctgctgcaat acgctgtggt taacgcgcca gtgagacgga tactgcccat
    29521 ccagttcttg tggcagcaac tgattcagcc cggcgagaaa ctgaaatcga tccggcgagc
    29581 gatacagcac attggtcaga cacagattat cggtatgttc atacagatgc cgatcatgat
    29641 cgcgtacgaa acagaccgtg ccaccggtga tggtataggg ctgcccatta aacacatgaa
    29701 tacccgtgcc atgttcgaca atcacaattt catgaaaatc atgatgatgt tcaggaaaat
    29761 ccgcctgcgg gagccggggt tctatcgcca cggacgcgtt accagacgga aaaaaatcca
    29821 cactatgtaa tacggtcata ctggcctcct gatgtcgtca acacggcgaa atagtaatca
    29881 cgaggtcagg ttcttacctt aaattttcga cggaaaacca cgtaaaaaac gtcgattttt
    29941 caagatacag cgtgaatttt caggaaatgc ggtgagcatc acatcaccac aatgaagcaa
    30001 attgtgaaca tcatcacgtt catctttccc tggttgccaa tggcccattt tcctgtcagt
    30061 aacgagaagg tcacgaattc aggcgctttt tagactggtc gtaatgaaat tcagcaggat
    30121 cacattatga cctttcgcaa ttgtgtcgcc gtcgatctcg gcgcatccag tgggcgcgtg
    30181 atgctggcgc gttacgagcg tgaatgccgc agcctgacgc tgcgcgaaat ccatcgtttt
    30241 aacaatggcc tgcatagcca gaacggttat gtcacctgga atgtggatag cctggaaagt
    30301 gccattcgcc ttggattaaa caaggtgtgc gaggaaggga ttcgtatcga tagcattggg
    30361 attgatacct ggggcgtgga ctttgtgctg ctcgaccaac agggtcagcg tgtgggcctg
    30421 cccgttgctt atcgcgatag ccgctccaat ggcctaatgg cgcaggcaca gcaacaactc
    30481 ggcaaacgcg atatttatca acgtagcggc atccagtttc tgcccttcaa tacgctttat
    30541 caactgcgtg cgctgacgga gcaacaacct gaacttattc cacacattgc tcacgctctg
    30601 ctgatgccgg attacttcag ctatcgcctg accggcaaga tgaactggga atataccaat
    30661 gccaccacca cacaactggt caatatcaat agcgacgact gggacgagtc gctactggcg
    30721 tggagcgggg ccaacaaagc ctggtttggt cgcccgacgc atccgggtaa tgtcattggg
    30781 cactggattt gcccgcaggg taatgagatt ccggtggtcg ccgttgccag ccatgatacc
    30841 gccagcgcgg ttatcgcctc gccgttaaac ggttcacgcg ctgcttatct ctcttctggc
    30901 acctggtcat tgatgggctt cgaaagccag acgccattta ccaatgacac ggcgctggca
    30961 gccaacataa ccaatgaagg cggggcggaa ggtcgctatc gggtgctgaa aaatattatg
    31021 ggcttatggc tgcttcagcg agtgcttcag gagcggcaaa tcaacgatct cccggcgctt
    31081 atcgccgcga cacaggcact tccggcctgc cgcttcatca tcaatcccaa tgacgatcgc
    31141 tttattaatc ctgaggcgat gtgcagcgaa attcaggctg cgtgtcggga aacggcgcaa
    31201 ccgatcccgg aaagtgatgc tgaactggcg cagctacgcg gtgaagattt ctcgcaactg
    31261 catattgtcg gcggcggctg ccagaacacg ctgctcaacc agctatgtgc cgatgcctgc
    31321 ggtattcggg tgatcgccgg gcctgttgaa gcctcgacgc tcggcaatat cggcatccag
    31381 ttaatgacgc tggatgaact caacaatgtg gatgatttcc gtcaggtcgt cagcaccacc
    31441 gcgaatctga ccacctttac ccctaatcct gacagtgaaa ttgcccacta tgtggcgcag
    31501 cttcactcta cacgacagac aaaggagctt tgcgcatgac cactcaactg gaacaggcct
    31561 gggagctggc gaaacagcgt ttcgcggcgg tggggattga tgtcgaggag gcgctgcgcc
    31621 aacttgatcg tttacccgtt tcaatgcact gctggcaggg cgatgatgtt tccggttttg
    31681 aaaacccgga aggttcgctg accgggggga ttcaggccac aggcaattat ccgggcaaag
    31741 cgcgtaatgc cagtgagcta cgtgccgatc tggaacaggc tatgcggctg attccggggc
    31801 cgaaacggct taatttccat gccatctatc tggaatcaga tacgccagtc tcgcgcgacc
    31861 agatcaaacc agagcacttc aaaaactggg gtgaatgggc gaaagccaat cagctcggtc
    31921 tggattttaa cccctcctgc ttttcgcatc cgctaagcgc cgatggcttt acgctctcgc
    31981 atcccgacga cagcattcgc cagttctgga ttgatcactg caaagccagc cgtcgcgttt
    32041 cggcctattt tggcgagcaa ctcggcacac catcggtgat gaacatctgg atcccggatg
    32101 gtatgaaaga tatcaccgtt gaccgtctcg ccccgcgtca gcgtctgctg gcagcactgg
    32161 atgaggtgat cagcaagaag ctaaaccctg cgcaccatat cgacgccgtt gagagcaaat
    32221 tgtttggcat tggcgcagag agctacacgg ttggctccaa tgagttttac atggggtatg
    32281 ccaccagccg ccagactgcg ctgtgcctag acgccgggca cttccacccg actgaagtga
    32341 tttccgacaa gatttccgcc gccatgctgt atgtgccgca gttgctgctg cacgtcagcc
    32401 gtccggttcg ctgggactgc gatcacgtag tgctgctgga tgatgaaacc caggcaattg
    32461 ccagtgagat tgtgcgtcac gatctgtttg accgggtgca tatcggcctt gacttcttcg
    32521 atgcctctat caaccgcatt gccgcgtggg tcattggtac acgcaatatg aaaaaagccc
    32581 tgctgcgtgc gttgctggaa cctaccgctg agctgcgcaa gctggaagcg gcgggcgatt
    32641 acactgcgcg tctggcactg ctggaagagc agaaatcgtt gccgtggcag gcggtctggg
    32701 aaatgtattg ccaacgtcac gatacgccag caggtagcga atggctggag agcgtgcggg
    32761 cttatgaaaa agaaattttg agccagcgtg ggtaaacact gccggatgcg gcgcgagcgc
    32821 cttatccggc ctacgggtcg tgcatctgac aacaccgaat ttacaggaac acagaacatg
    32881 caaaacatta cccagtcctg gtttgtccag ggaatgatca aagccaccac cgatgcctgg
    32941 ctgaaaggct gggacgaacg caacggcggc aacctgacgc tgcgcctgga tgacgccgat
    33001 atcgcgcctt ataaagacaa cgtccatgca caaccgcgct atatcccgct cagccagccc
    33061 atgcctttac tggcaaatac accgtttatt gtcaccggct ccggcaaatt cttccgtaac
    33121 gtccagctcg atcctgcagc taacttaggc gtcgtaaaag tcgacagcga tggcgcgggc
    33181 taccatattc tctgggggtt aaccaacgaa gccgtcccga catccgaact cccggcgcac
    33241 tttctctccc actgcgagcg cattaaagcc accaacggta aagatcgggt gatcatgcac
    33301 tgccacgcca ccaacctgat cgccctcacc tatgtactgg aaaacgacac cgcggtcttt
    33361 actcgccaac tgtgggaagg cagcaccgag tgtctggtgg tattcccgga tggcgttggc
    33421 attctgccgt ggatggtgcc cggcacggac gaaatcggcc aggcgaccgc acaagagatg
    33481 caaaaacatt cgctggtgtt gtggcccttc cacggcgtct tcggcagcgg gtcgacgttg
    33541 gatgaaacat tcggtttaat cgacaccgca gaaaaatcag cacaagtatt agtgaaggtt
    33601 tattcgatgg gcggcatgaa acagaccatc agccgtgaag agttgatagc gctcggcaag
    33661 cgcttcggcg ttacaccact agccagtgcg ctggcgctgt aaggagcaaa catgatccgc
    33721 aaagcctttg tcatgcaggt taaccccgac gcccacgaag agtatcagcg tcggcataac
    33781 cccatctggc cggaactgga agcagtgttg aaatctcacg gtgcgcataa ctacgccatt
    33841 tacctcgaca aagcgcgtaa tctgctgttt gccacggtag agattgaatc tgaagaacgg
    33901 tggaatgcgg tagccagtac tgatgtttgc caacgttggt ggaaatatat gaccgatgtt
    33961 atgcccgcta acccggataa cagcccggtg agtagcgagc tg
//
LOCUS       DAZPEB010000021        33473 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZPEB010000021 DAZPEB010000000
VERSION     DAZPEB010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33473
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.021"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            520..759
                     /locus_tag="ACHURJ_000912"
     CDS             520..759
                     /locus_tag="ACHURJ_000912"
                     /inference="COORDINATES: protein motif:HMM:NF020425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA family protein"
                     /protein_id="HFR1279964.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
     gene            complement(947..1924)
                     /gene="intI2"
                     /locus_tag="ACHURJ_000913"
                     /pseudo
     CDS             complement(947..1924)
                     /gene="intI2"
                     /locus_tag="ACHURJ_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            2256..2729
                     /gene="dfrA1"
                     /locus_tag="ACHURJ_000914"
     CDS             2256..2729
                     /gene="dfrA1"
                     /locus_tag="ACHURJ_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HFR1279965.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            2824..3348
                     /gene="sat2"
                     /locus_tag="ACHURJ_000915"
     CDS             2824..3348
                     /gene="sat2"
                     /locus_tag="ACHURJ_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HFR1279966.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            3406..4241
                     /gene="aadA1"
                     /locus_tag="ACHURJ_000916"
                     /pseudo
     CDS             3406..4241
                     /gene="aadA1"
                     /locus_tag="ACHURJ_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
     gene            4270..4767
                     /locus_tag="ACHURJ_000917"
     CDS             4270..4767
                     /locus_tag="ACHURJ_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1279967.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            4828..5199
                     /locus_tag="ACHURJ_000918"
     CDS             4828..5199
                     /locus_tag="ACHURJ_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1279968.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            5609..5986
                     /locus_tag="ACHURJ_000919"
     CDS             5609..5986
                     /locus_tag="ACHURJ_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1279969.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            6009..6161
                     /locus_tag="ACHURJ_000920"
                     /pseudo
     CDS             6009..6161
                     /locus_tag="ACHURJ_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(6217..7833)
                     /locus_tag="ACHURJ_000921"
     CDS             complement(6217..7833)
                     /locus_tag="ACHURJ_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn7-like element transposition protein TnsE"
                     /protein_id="HFR1279970.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALEQSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(7834..9360)
                     /locus_tag="ACHURJ_000922"
     CDS             complement(7834..9360)
                     /locus_tag="ACHURJ_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsD family Tn7-like transposition protein"
                     /protein_id="HFR1279971.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQG
                     AVHLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVF
                     FDRAVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQW
                     TLFYQRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFR
                     KHRKAFSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVK
                     HKDWQQLVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDW
                     NQRDRIAVRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKR
                     YSESVEDYQIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE
                     "
     gene            complement(9363..11030)
                     /locus_tag="ACHURJ_000923"
     CDS             complement(9363..11030)
                     /locus_tag="ACHURJ_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR1279972.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            complement(11027..13135)
                     /locus_tag="ACHURJ_000924"
     CDS             complement(11027..13135)
                     /locus_tag="ACHURJ_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR1279973.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            complement(13122..13943)
                     /locus_tag="ACHURJ_000925"
     CDS             complement(13122..13943)
                     /locus_tag="ACHURJ_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsA endonuclease N-terminal domain-containing
                     protein"
                     /protein_id="HFR1279974.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            complement(14103..15932)
                     /gene="glmS"
                     /locus_tag="ACHURJ_000926"
     CDS             complement(14103..15932)
                     /gene="glmS"
                     /locus_tag="ACHURJ_000926"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFR1279975.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIMVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(16094..17464)
                     /gene="glmU"
                     /locus_tag="ACHURJ_000927"
     CDS             complement(16094..17464)
                     /gene="glmU"
                     /locus_tag="ACHURJ_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFR1279976.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILISNGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDVNLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(17815..18234)
                     /locus_tag="ACHURJ_000928"
     CDS             complement(17815..18234)
                     /locus_tag="ACHURJ_000928"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFR1279977.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQSGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(18255..19637)
                     /gene="atpD"
                     /locus_tag="ACHURJ_000929"
     CDS             complement(18255..19637)
                     /gene="atpD"
                     /locus_tag="ACHURJ_000929"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFR1279978.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(19664..20527)
                     /gene="atpG"
                     /locus_tag="ACHURJ_000930"
     CDS             complement(19664..20527)
                     /gene="atpG"
                     /locus_tag="ACHURJ_000930"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFR1279979.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHLYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(20578..22119)
                     /gene="atpA"
                     /locus_tag="ACHURJ_000931"
     CDS             complement(20578..22119)
                     /gene="atpA"
                     /locus_tag="ACHURJ_000931"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFR1279980.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(22132..22665)
                     /gene="atpH"
                     /locus_tag="ACHURJ_000932"
     CDS             complement(22132..22665)
                     /gene="atpH"
                     /locus_tag="ACHURJ_000932"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFR1279981.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(22680..23150)
                     /gene="atpF"
                     /locus_tag="ACHURJ_000933"
     CDS             complement(22680..23150)
                     /gene="atpF"
                     /locus_tag="ACHURJ_000933"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFR1279982.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(23212..23451)
                     /gene="atpE"
                     /locus_tag="ACHURJ_000934"
     CDS             complement(23212..23451)
                     /gene="atpE"
                     /locus_tag="ACHURJ_000934"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFR1279983.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(23498..24313)
                     /gene="atpB"
                     /locus_tag="ACHURJ_000935"
     CDS             complement(23498..24313)
                     /gene="atpB"
                     /locus_tag="ACHURJ_000935"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFR1279984.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(24322..24702)
                     /gene="atpI"
                     /locus_tag="ACHURJ_000936"
     CDS             complement(24322..24702)
                     /gene="atpI"
                     /locus_tag="ACHURJ_000936"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFR1279985.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(25319..25942)
                     /gene="rsmG"
                     /locus_tag="ACHURJ_000937"
     CDS             complement(25319..25942)
                     /gene="rsmG"
                     /locus_tag="ACHURJ_000937"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFR1279986.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(26006..27895)
                     /gene="mnmG"
                     /locus_tag="ACHURJ_000938"
     CDS             complement(26006..27895)
                     /gene="mnmG"
                     /locus_tag="ACHURJ_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFR1279987.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(28274..28717)
                     /gene="mioC"
                     /locus_tag="ACHURJ_000939"
     CDS             complement(28274..28717)
                     /gene="mioC"
                     /locus_tag="ACHURJ_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFR1279988.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(28807..29265)
                     /gene="asnC"
                     /locus_tag="ACHURJ_000940"
     CDS             complement(28807..29265)
                     /gene="asnC"
                     /locus_tag="ACHURJ_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFR1279989.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQHPIMRTIKP"
     gene            29417..30409
                     /gene="asnA"
                     /locus_tag="ACHURJ_000941"
     CDS             29417..30409
                     /gene="asnA"
                     /locus_tag="ACHURJ_000941"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFR1279990.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVIHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYLDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(30414..31865)
                     /gene="viaA"
                     /locus_tag="ACHURJ_000942"
     CDS             complement(30414..31865)
                     /gene="viaA"
                     /locus_tag="ACHURJ_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFR1279991.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWSEALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(31859..33355)
                     /gene="ravA"
                     /locus_tag="ACHURJ_000943"
     CDS             complement(31859..33355)
                     /gene="ravA"
                     /locus_tag="ACHURJ_000943"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFR1279992.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
BASE COUNT         8528 a         8453 c         7808 g         8684 t
ORIGIN      
        1 tcggcagccc tgtgggcgga caaaatagtt gggaactggg aggggtggaa atggagtttt
       61 taaggattat ttagggaaga gtgacaaaat agatgggaac tgggtgtagc gtcgtaagct
      121 aatacgaaaa ttaaaaatga caaaatagtt tggaactaga tttcacttat ctggttaact
      181 aatctagcta acctgccgct cagcttagta cgatcctata tccaactgat cataatcctt
      241 tcatcagtat cccaaattaa atccccctcc tagactgcct caccaacact ttacagtgat
      301 cccgtatttc cggacaggtg attgattttt ttcgcttgta caggtgttaa gccacagtta
      361 tcttgatgtg gacattatgc attgtaatag cccattaaca tgcgctaaga gcataaatta
      421 atatacagta acaagcatta cgaggtgcta atgtttggaa agcagcctct tccaactaca
      481 ataataagtt aactatcctc cacccacaaa taattacaaa tgaccccttt ccgctataac
      541 tccgatctca ccagcggctc gctacaaacc cgagagtgtc gcattattac tggcttgtta
      601 ctccaagagc ttgatgaagc tgcctgggat aaagccatgt ataaggaaaa tgtgcttcag
      661 aaacgcacgc aatctacggt aagacgtatt tcttcagcgc ttagaaaacg cctagaacac
      721 ctaagctctg acttttgggc ttttgcgttt ttatgttagg cattgtctgg caactggagg
      781 aagtgtaatg accatgaatg gtcactgtga ttgacagtat cttgcttaaa cttatctgaa
      841 caatggtaga actggctcat atttatcccc tctatgattg tttatctttt tcaccataaa
      901 aagtatcagg ggctactcag ggctacataa agatatttat ctacttttac tgattgataa
      961 gtagcatcag tccatccgca ggactggtgg tgccggcaaa atgctgaccc aacacatgcg
     1021 tatagatttg cgtggtctta acatcgttat gccctaagag ttcttgcaca gtgcgaatat
     1081 cacgccccgc ttgtaataga tgcgtagcaa acgagtgacg aaatgtatga caagtgacac
     1141 gcttgctaac gatgcctgct ttttgtacgg ctgccttcaa tgcctttcgc gcaacggagt
     1201 catgcagatg atggcggcat aatttgccgt tatacgggtg gttgcagagc gtgctggagg
     1261 gaaagacaaa catccacgcc gcttgtcgat aagcagaagg gtatttgtga tctaaagcaa
     1321 aaggcagcga tggccctacg ccttgtaagt tgtcgtcttg ctgaataagc cgcgcttgct
     1381 caatgagtta ttttattgct gggattaggc gcgtgggcag taggctgttt ctgcttttcc
     1441 cacccttacc gtcatgcaca gtgatgcagc cattatcaaa atcaaaatct ttaacccgca
     1501 aacgcaagca ttcattaatg cgcaaacctg caccatacag cagcgtaaaa ataacttggt
     1561 tgcgagtatc cataacctgc aaaatgcgtt gcacttcatt tgcagagata acagagggta
     1621 gccgtctagg cttgcttgca gggatataat caatatcgcc caacggctgt tgtaaaaacc
     1681 tgttgtacaa aaaagctagg gcatttaaag cgattttctg cgtgtttatg gctacatgtc
     1741 tgctgtttgc taagctggat aaaaacagcc tgacctcttc actgcccatg gtctgaggat
     1801 gacgtttttt gtgaaacaga ataaaacgct taatccagtg caggtaagtt ttttcagttt
     1861 tcagcgcata acctttttgt cgcatatccg tgcgtataga atttaaaaat ggactgttag
     1921 acataaaacg ctccttgtct tgcaactgtc tgcctataca gcctattcta gctgggattt
     1981 aaaaaagtgc ctgtttttta cgcctagaga tgcttgttta ccggtagagt tttaatttaa
     2041 tgctaaataa attaaaatgt tatgagttct ttgggtgaga taatgtgcat cgtgcaagca
     2101 ggatagacgg catgcacgat ttgtaataac agagtgtctt gtatttttaa agaaagtcta
     2161 tttaatacaa gtgattatat taattaacgg taagcatcag cgggtgacaa aacgagcatg
     2221 cttactaata aaatgttaac ctctgaggaa gaattgtgaa actatcacta atggtagcta
     2281 tatcgaagaa tggagttatc gggaatggcc ctgatattcc atggagtgcc aaaggtgaac
     2341 agctcctgtt taaagctatt acctataacc aatggctgtt ggttggacgc aagacttttg
     2401 aatcaatggg agcattaccc aaccgaaagt atgcggtcgt aacacgttca agttttacat
     2461 ctgacaatga gaacgtattg atctttccat caattaaaga tgctttaacc aacctaaaga
     2521 aaataacgga tcatgtcatt gtttcaggtg gtggggagat atacaaaagc ctgatcgatc
     2581 aagtagatac actacatata tctacaatag acatcgagcc ggaaggtgat gtttactttc
     2641 ctgaaatccc cagcaatttt aggccagttt ttacccaaga cttcgcctct aacataaatt
     2701 atagttacca aatctggcaa aagggttaac aagtggcagc aacggattcg caaacctgtc
     2761 acgccttttg taccaaaagc cgcgccaggt ttgcgatccg ctgtgccagg cgttaggcgt
     2821 catatgaaga tttcggtgat ccctgagcag gtggcggaaa cattggatgc tgagaaccat
     2881 ttcattgttc gtgaagtgtt cgatgtgcac ctatccgacc aaggctttga actatctacc
     2941 agaagtgtga gcccctaccg gaaggattac atctcggatg atgactctga tgaagactct
     3001 gcttgctatg gcgcattcat cgaccaagag cttgtcggga agattgaact caactcaaca
     3061 tggaacgatc tagcctctat cgaacacatt gttgtgtcgc acacgcaccg aggcaaagga
     3121 gtcgcgcaca gtctcatcga atttgcgaaa aagtgggcac taagcagaca gctccttggc
     3181 atacgattag agacacaaac gaacaatgta cctgcctgca atttgtacgc aaaatgtggc
     3241 tttactctcg gcggcattga cctgttcacg tataaaacta gacctcaagt ctcgaacgaa
     3301 acagcgatgt actggtactg gttctcggga gcacaggatg acgcctaaca attcattcaa
     3361 gccgacaccg cttcgcggcg cggcttaatt caggagttaa acatcatgag ggaagcggtg
     3421 atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa
     3481 ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac
     3541 agtgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct
     3601 ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct
     3661 gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc
     3721 gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc
     3781 acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg
     3841 gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg
     3901 ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat
     3961 gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag
     4021 gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt
     4081 gaagctaggc aggcttatct tggacaagaa gatcgcttgg cctcgcgcgc agatcagttg
     4141 gaagaatttg ttcactacgt gaaaggcgag atcaccaagg tagtcggcaa ataatgtcta
     4201 acaattcgtt caagccgacg ccgcttcgcg gcgcggctta actcaagcgt tagagagctg
     4261 gggaagacta tgcgcgatct gttgaaggtg gttctaagcc tcgtacttgc gatggcatcg
     4321 gggcaggcac ttgctgacct gccaattgtt ttagtggatg aagctcgtct tccctatgac
     4381 tactccccat ccaactacga catttctcca agcaactacg acaactccat aagcaattac
     4441 gacaatagtc catcaaatta cgacaactct gagagcaact acgataatag ttcatccaat
     4501 tacgacaata gtcgcaacgg aaatcgtagg cttatatata gcgcaaatgg gtctcgcact
     4561 ttcgccggct actacgtcat tgccaacaat gggacaacga acttcttttc cacatctggc
     4621 aaaaggatgt tctacacccc aaaagggggg cgcggcgtct atggcggcaa agatgggagc
     4681 ttctgcgggg cattggtcgt cataaatggc caattttcgc ttgccctgac agataacggc
     4741 ctgaagatca tgtatctaag caactagcct gctctctaat aaaatgttag gcctcaacat
     4801 ctagtcgcaa gctgagggga accactagtg tcatacgaac ctccaagaga cggttacaca
     4861 aacgggtaca ttgttgatgt catgtatgac aatcgcccaa gtaagtatcc agctgtgttc
     4921 agaacgtacg tccgaattcc agactatgtc gaactagata acgcggagga gctggccgac
     4981 gcaatcattc gagaggttga gttaaaggag gcatggctca caagggtctt ggggaactca
     5041 gccgatcctg acgcttctgt gggcaagtgg gaccggcgcc tagcgcagtg cggtggtatt
     5101 gatgagatgt caagtggggt gcaggcaaca tacttcagtc caaacggtca gctggtcggc
     5161 ttagtggcat acattgatgc cgaatggatg gatgaatagc tctgacgtta cgattcaggt
     5221 gagacaacca gatgggcatt gcgtacaagc tcagttgcca ccgatgtggc tacgagtctg
     5281 atcttcttta cctaggccaa ggtatggcga tgcttcctga gcaaatagtc ggaacgtgca
     5341 catgctgcga caatctaacg acgatttcta taaatgaaag ctacggagta tgctcacttt
     5401 gtggggctaa ggatcgtgtg atatttacaa gctatcagca tgaggcaagg ccaagaaatc
     5461 cctgctatct agagagaagt ttcaaatatc aatgcccaag atgccatgcc ttcagtatgg
     5521 agcctccgga tctaccggag atgctttggg actaatgctc cgctttgcct cgggtatccg
     5581 ttcgacctat gtttaagagg aaacgcacgt gtctgccgcc aaagctcttg cctatgcact
     5641 ttgtgacaag ttggaaagca aaaaagttga tgaagtcgta cgaaatcttg tttctagcgg
     5701 ctggaatatt agggaagtgg cctgggaagc ttcgtccttt gatcatgaaa tgccctactt
     5761 tgacacgggg cgggacttcg atattggatt tcctgacacg ctgtcatcca agattgagtt
     5821 tttgaccgcg aatggatcaa tcgtccaagc acgtgctcgc ctgcacttca agcaggctct
     5881 aatttttgcc aaggcgtcca aacatgtcgc cgcgctcaag aactcattgg attactacta
     5941 tggatcgtct gtaatccccc ctcaaaacca cggaggctat ttgtagaaaa ttccattaat
     6001 atgaactcaa ggtgttccct acggacgaat cagcgatgaa ggtggtgtac ctggctatcc
     6061 aggcggcgac caagaaatgg acgatgccga tccgcaactg gaagccggcc atgaaccgtt
     6121 ttatgatcga atttggtgac cgtttaaatg gccaccttta actgaccggc aaaagcactt
     6181 acacaaaagg gtttacaggc tctttcagtc attcctttaa tgcgtaaatt gctctctaac
     6241 cactctctca gcccaccctt ccatatcctc tctcgatact tgcagctccc ccaacccctt
     6301 tggatgattc acccctctat gcccgtcttg tccatataat tgatcaaaca aactatttgg
     6361 ccaattcaat gagctcttca ccactccacg ccgtatcttt tcaaaatcat tccgccatgt
     6421 ttcgctatca acgccactca acactttggt agaaagcatt ttcaccccat ccgatgcatc
     6481 cacctctagc aatacaaatt cactgccatt acgttgcacc ccaacggctt taatcactct
     6541 tggtgagcca tctttacaca gatgtaatcg gctacgccca acttttggca aaaccaaggt
     6601 ttcttcaaaa agcacccgac atgcaaattt agtttttaga attgaaagta gctcatcaaa
     6661 agcagcaaat cgattagcaa aaatagagtt gtaattggtt gcatcctgct tcccacccac
     6721 atctgccgct gctaggacac cacctaagtg tggctcatca gtactaacca aattactatt
     6781 ttcacttgtt gttgcttctt ctttttctaa aatagtttgg ctactttttc ttgccctgcg
     6841 agatttttca attcgactag gtctactaaa acttatccat gtcggctcag cctctatgac
     6901 tcgtgtgtct gtgtctatat tggctgtttc ctcatcgtcc aactgtagat gctcatcatc
     6961 aacaactgac tctgttgaaa ccgctatgtg ctgcgtacta ccatcacctg cctccttttc
     7021 ctgaaaagag gcatggctaa tagctgttgt gctaggaagc atagcgttga tttctaaacc
     7081 tactatttcc tcaactaaat aatccttatc ctcgttagaa gaacgtcctt ttacatgtaa
     7141 tttccaaccc tgcatgggcg gagggtcaaa gctaaagcac cagctttcaa cgccgttctt
     7201 aatttctcta ttttgttgat agtgcctaaa aatactttca tacgaatcca taacatcttg
     7261 atccgaaaac aaccaaacca aaagctgcac tacggccgac tgctctaacg cccctttagg
     7321 aaacgatgaa ctgggtaaga tccttatctc taaatgatct cgctcaacct catactgaac
     7381 gtcgaactct tgctgtaaag cggtactgct caaacagctt cgacagaaat aggagttaat
     7441 taaaaataat gaccgagcta gctctaactg tgggatgtga taacgagtct tgctatatgg
     7501 tgtttcagct tcgtaaataa atgaactctg cgctccatcc ttgttgcgta cggaagcaaa
     7561 ggacgagaga ttaggaaaat cgattatttt ggctcgttga agatccgatg cttcaaactc
     7621 aatcacacga tccgccttat ttatttgctt tgttgtactg ttaaccacct tagctcgact
     7681 taataaaggc aaatgagtaa atcgagtcca tttccgttct tgcattggat taaaccaaac
     7741 aaaaatacgc cactccttat gacccgagtt acggaataaa tgaccaatat gtacaacctg
     7801 cacattgtca ttaaatgtag ctagcctaac cactcactct tccccataaa ccatttccaa
     7861 gaatctttgt gcttcctcag ttatccgctc tttagataac gttgcacttc ttaataatcg
     7921 ccagcgccta agctcaacat cttcctgttt aagcttaata aaagcttggc taatccgtct
     7981 aatttgataa tcttccacac tctctgagta acgctttaag caaagcgcta ccaaaggcag
     8041 tttctgtaga ttttttgcaa gagaggttcc gttaggagtt tgctttaaca gccagctcga
     8101 tgtcgctctt gggtgatcaa ggctactatc tagacgcttt atgattctta atagttgtcg
     8161 tacagcaatt cgatctcttt ggttccaatc aactctaggg gcgggtgcca gacgctcttg
     8221 ttgatgctgt tgattccagt gaactagcca atccctgtca tgtcggtaaa gccaagcgta
     8281 tagcacccca ccctctaaag actgtcttgc cgccttaatt ccttggtatt tatgcactag
     8341 ttgctgccag tctttatgct taacagataa atcttcagag ttaggttgca cagactggct
     8401 aacaggtctt gtcgttatag agtgctcagt aagagcactt gcctgctgta gcgcttctat
     8461 aaccgttagt tttggcaata aggcttgcca cacaatacta tgctgtaaat aactaaaggc
     8521 ttttctatgc ttacggaata tacttttcag ccaacacgtg tccttgttct ctgccaactt
     8581 taaatccagt ttctctagtg cctcatcact aaaagtctgc ctcactctct ccgccaccaa
     8641 gtcatgacga atgtgcttgc ttttggttag ccctagatcc tgcgctaagc gctgataaaa
     8701 cagcgtccac tgctcaaggc ttggggaaag ctcttgcgct cgtggagcat ctaacagagg
     8761 ggctatataa gcagctagtg ctgttaattg agatagggag tctttggggt agtctgaaag
     8821 cagctcagta tgacccaaag cccaaaattg atgtcggtga tcatctacag ctctatcaaa
     8881 gaagactaaa gcaccgtgtt ttggacaata tggcaaagcg ggcaaatacc aatctcgttg
     8941 ccaaaaggct tccccatacc tatttagctg aagagcaacg caatcagggc agtatctaaa
     9001 gcggttatcg ctcttaactc tagaagcagc gactcctagc attaaatgca ccgcaccttg
     9061 cgcttggtac tccattaacc gaatagcttc gtctcggcgc tccttgccta caaacggagc
     9121 atataaaggg aataaggtat gctcataaat aagctgctga acagcgtaac gtcctgtttg
     9181 atgtagatgt cttgctatca cacctaaatg cgagggcaga cctaaggtag cgaccacctt
     9241 gcggttgcca tacacctcat ccaacagctg cttaggacta acaatccctt gataaacgcc
     9301 tgcccgtgca atagtgctat aaatcagctc attcgagtac ggaacaggaa agtttctcat
     9361 ggctaacctg cttgtttaaa taagctcgcc atatccacta ttaccccttt cccttttaac
     9421 cgttcatgca tggttttttc aggttggcgt tgtgaataga tataacgtaa atccgtatca
     9481 ggcaagctat cccagtcgct gggcttgact acctttatag ccgaaacctt tttactcttg
     9541 gagggctttt ctagttctga cactaccgtt tcgccttcca tcaaccactg caaaacaaga
     9601 ggcagtaact tttgtcttgt catcgttgga ttctggctaa acgctttttt aatagtggga
     9661 attaacaggc ttgaatcgta atcctctttc agcatcagat ataaatgacg ctgatcttcg
     9721 gtatctaact cttgaagggc tttttcttct ggtgtttgtt cttgtatcgc tgcgatatct
     9781 agctgaagtt ggattaaccg tttatcaatc tcgggaacga ctagatcaga ataacgagca
     9841 atgcgttctg ggatacccga gcgtaatgcc tctagcatgg ggtgcacagg ctttaactca
     9901 tcttgataca cttgccgcaa taaaccagcg gtaatacgct cattgcctaa agctagcgca
     9961 cggagctgag cgagtacaaa aagttttact acaatgtcca tcactccttg gcttagctca
    10021 taccacacat cacggacctc atccgataac agcgcatctt tgcgttgtaa aagctgtaat
    10081 tgccaaagat tatccgtaaa agcgatccac tcttgattgg gctttccacg ttgcgtttgt
    10141 tgtataggat cccagaatat agctccaaac cctgcccctc tacgtgcaga ccgcaaatca
    10201 gcctcaaaaa tctctcgtgc tttaggggta ccaatcaaca tcactggtac gccaataata
    10261 ttcaccatcg tcacaaaaaa gttcagcatc tcttgagatc cacccgaacg agagcggctt
    10321 aaatgctgaa tttcatcaat aaccaacaac cctaaagcat gtgcattggc tatttgcgac
    10381 atcaaagcca acatggtttc tataccatga cgttttaagc cataacgacg ctcatagttc
    10441 gagcccaagg ctcgatccaa cgctctgaaa aaattcaagc agatttcttt tagcgaacca
    10501 ttatgcgagc agtctatttt caaatacacc acctgctcta cattgagttc acgatggtaa
    10561 atcacctgag gatacgtggc tagaatacga tgaagagagg tcgtcttccc actaccagaa
    10621 caaccaatta acaataagct ttgtgccgta gatcgtgcct cctcaaagcg aaatgtctcc
    10681 aactctcccg tttgaacacg ctcataacca ttttgtaaat gcttttgtaa atctcctgtt
    10741 ttaggatttc tgcctacgta gccacctcga atcatgaccg aaatacgctc acttagtagc
    10801 aaatgcgtac ctaatggctg aaaatagtca tctggaatac gacaaatggt atgagctctg
    10861 ataacacggg acttttgcaa gtcagaggaa gtaagctgta aagaggattt cagtgatgca
    10921 gcactattca ctgactcttg taatggtggt aaggcctcga taaaaggatt atcacgataa
    10981 gcctctaccc ccgtatcacg ataaactgct tgaatccggg tagcactcat gactcatcct
    11041 tttctggtgg atcctgaaat aattcaggca cgtatgtggg taggctgtaa tcttcttgat
    11101 catccgcttc cactgcgttg aaaggaataa ctttagcctc atcacctgaa gagcttggct
    11161 tcaaatgctc cgcacgtttt ttacgctccg aggtcacggc ttctttttta ttagttttaa
    11221 tctgcttaat gcgtgttgat ttgggctcag tagtactggg cgttaactta ttcgctttct
    11281 gaatggtttg ctgaataaac gcctcaagct ccctgcgttt agttaactca tcctgcttcg
    11341 cattggcttt attgtgtttt tcttgtgctt gtatatccca aacctcccaa aatgagagac
    11401 ctttaaactg ccgactacgt tccgtcagat tacagcgcca aaatacacgg ctgccaactt
    11461 gcggaaacaa ataaatcgta tcaaccagca ctgggtcata agccgcttct aaatgttgag
    11521 gtctagctat atcagtgctc cgctgcaacc aaccctcacg cagaatctct gaccccgagt
    11581 aatacaaacc ccacaaatta acgccaaatg aagaaataga gacctttcgg cgaggcagta
    11641 acgctactcg caactgctct tgctccacag cccttaaact acctgtacga tgctgcatac
    11701 cccattgcca tagctggaca ggaatagacg gtaaatctgt aggaaaatca gcatctcgat
    11761 cgtatttatc catcaccaga tggttatttc tgaataagat cgtacgcaaa ataatttgtg
    11821 tgaactcaaa taccgacaga gatgcatcta acctatagtc tgtttcacca tggcttttga
    11881 tccgactgcc ctctacaatg ccaggtgcaa aggacttaaa ctcggcttgt agtgttctaa
    11941 aagtgctttc cactatgcct ttagcatcgc cacgtctagg tggagcactt tccactcgca
    12001 cattaaaact agaaactaag gcttcgacct gatgactcat taattcgcca cggtccgcta
    12061 gcaacacatc tggcaaacct acacacggcc agtctgagct actaatctca atatcatgct
    12121 gggcacaaat ggccgttttg tcagagcaag catttacaaa agcctgcatc gccaccacat
    12181 aagacggatt ttcaaagccg atataaaagc ccgtgatcat ccgactaaac acatcaatca
    12241 caatgtaaag cgttggtctt cctatgattt tttggcgatc atgatgatcc actaaataaa
    12301 tatcagcaat cgtggcatca atctcataac gactcccagg gcctaacgcc tgagaagtgg
    12361 ctgtactact taagggtcgt acgtcttttt tatatacccc tgctttaact ctagacttta
    12421 agcgctgagc tttagggtat tctcgatcat aaaaataacg aaactgacgt agtgttgggt
    12481 aatcctcttg gggaatgcga ggaaaatact gagcaaacaa gtccacaaat cgtctatagg
    12541 caacggtggt ctttgtacct ttttgattta acaggtgctt ttctatggtc aacctaaaaa
    12601 ggcgttcaat ctcgggcgtt accttggttc cttcaccctt accatattct ctggctcggc
    12661 caatctttgc tgttcctgtc gctgaacgtc tttcccctgg tgcaccgctg tttttgtagt
    12721 caggaattaa tgcattaggc gtttgaccac gctgccagta acggcgtaac aacttataaa
    12781 ctgtagcctt agtaacctta tgttcttgga ccacatgctc aacgagttcg cttctgactt
    12841 tagggtcgaa acgatcctta ctattaataa taggaagaat ttttcgataa tcctcctcgc
    12901 gcttctggaa gctgacagaa tctacagacg gctcttctaa atcaagatgt acataagggt
    12961 catcagttct aactaagcgg ccttcatcta aatactgcat tagcaacaac tcagccctag
    13021 cttgtggaac tcctttatca gcgcttattt gcatccagac aacttggcca tcctctatag
    13081 ccaaaatgcg atacggatca ttatcaaata gcacaacctc attaatttgc cacatagcgc
    13141 aactcctcca tattcactac ttggctaata cagagatctg cacacttatt tgccctgaaa
    13201 gacttataaa tattgaactt aataaaacca tttgcggtta aggctcgtat ctcactcaat
    13261 gttttgccta actccaaatc ataagcaata tcaacctgct tacagacatt gataatgttt
    13321 tcatctcctt tttcttgcag gatatgggcc aatggggata gttgtgctaa aagctccgca
    13381 gaaacttctt ctgttttcac tgaataaagc cattcaatat tttcttttac tacgggattt
    13441 atttctttat cagtaaaaat gaaccaagga atttgctttt gctgccaata gcgacgctct
    13501 agttctagtt tttctaaggt acgctcgtct tgtaaggctg ctgcaggttt gacttgaata
    13561 gcaaactgct caaaaggacc atctttgcag tccactaaaa aatcagtaga cataacctga
    13621 tctacaccac gaataacagg atgcttaata ccactatcta ttgcaatctg cctggtatca
    13681 ctaggtaata aggggaactg ctcgcgtata tctagcacgc tgctctccca ctcaagactg
    13741 agaaaaacag caagctctaa gtcagatagc aaatgatgga ctcgtcccgt cttatgagaa
    13801 taaatacggt gggaacgacc tgaagaagga acttcttgta ctgttagcca tggaatatag
    13861 tctttaccat gcccttggcc acgcccctct ttaatacgac gggcaatttg cacttcagaa
    13921 aatgaagagt ttgctttagc cataacaaaa gtccagtatg ctttttcaca gcataactgg
    13981 actgatttca gtttacaact attctgtcta gtttaagact ttattgtcat agtttagatc
    14041 tattttgttc agtttaagac tttattgtcc gcccacaagc ccttttttat ctatgatagt
    14101 gattactcaa ccgtaaccga ttttgccaag ttacgcggct ggtcaacgtc ggtgcctttg
    14161 atcagcgcga catggtaagc cagcagctgc agcggaacgg tgtagaagat cggtgcaatc
    14221 acctcttcca catgcggcat ctcgatgatg tgcatgttat cgctacttac aaaacccgca
    14281 tcctgatcgg cgaagacata caactgaccg ccacgcgcgc gaacttcttc aatgttggat
    14341 ttcagttttt ccaacagttc gttgttcggt gcaacgacga taaccggcat atccgcgtca
    14401 atcagcgcca gcgggccgtg tttcagctcg ccagcagcgt aggcttcagc gtgaatgtag
    14461 gagatctctt tcagcttcaa tgcgccttcc agtgcgattg ggtactgatc gccacggccc
    14521 aggaacagtg cgtgatgttt gtcagagaaa tcttctgcca gagcttcaat gcgtttgtcc
    14581 tgagatagca tctgctcaat acggctcggc agcgcctgca gaccatgcac aatgtcatgt
    14641 tcaatggagg catccagacc tttcaggcga gacagcttcg ccaccagcat caacagcaca
    14701 gttaactgag tggtgaatgc tttagtggac gccacgccga tttccgtacc cgcgttggtc
    14761 atcagcgcca gatcggattc gcgcaccaga gaagaacccg gaacgttaca gattgccagc
    14821 gaaccaaggt aacccagctc tttcgacaga cgcaggcccg ccagggtatc cgcggtttcg
    14881 ccagactgtg acaaggtgat catcaggctg ttacgacgca cggcagattt acgatagcgg
    14941 aattcagagg cgatttcgac gtcgcacgga atacctgcca gcgattcaaa ccagtagcgg
    15001 gaaaccatac cggagttata agaagtacca caggcgagga tctgaatatg ctcaaccttc
    15061 gacagcagtt cgtcggcgtt cggtcccagc tcgcttaaat caacctgacc gtggctgatg
    15121 cgcccagtaa gggtgttttt gatcgcgttc ggctgttcgt agatctcttt ctgcatgtag
    15181 tgacggtaaa tacctttatc gcccgcgtca tattgcagat tggattcgat atcctgacgt
    15241 tttacttccg cgccagtttt atcgaagatg tttaccgaac ggcgagtaat ttccgcaata
    15301 tcgccctctt caaggaagat aaagcgacgg gtcaccggca gcagcgccag ctggtcagaa
    15361 gcgataaagt tttcgcccat acccaggcca atcaccagcg gactaccaga acgtgccgcc
    15421 agcagggtat ccgggtgacg ggagtccatg atcactgtac cgtacgcacc gcgcagctgc
    15481 gggatagcac gcagaacagc ctcacgcaga gtcccgcctt gttttagctc ccagttcacc
    15541 agatgggcaa tcacttcggt gtcggtttca gaaacgaagg tatagccacg cgcttttaac
    15601 tcttcacgca gcggttcatg gttttcgatg atgccgttat gcaccaccat aatgtgttca
    15661 gaaacatgcg gatgcgcgtt agcttctgaa ggttcaccgt gggtcgccca gcgagtgtga
    15721 gcaataccgg tgccgccatg cagaggatgt tcttccgctg cctgagccag catctggact
    15781 ttaccgaggc gacgcaggcg ggtcatatga ccttccgcat caacaacggc cagaccagca
    15841 gagtcatatc cgcggtattc cagacgacgt aaaccttcaa gaaggatttc tgctacatca
    15901 cgttgcgcga tcgcgccaac aattccacac atagtttttg attccgattt atatcgttgt
    15961 cggtcaacct gtatgcccgt tttttcgggc gccccgagcc ttgtagagag tggggttatt
    16021 tttatagtta ctgcttgtgg gagggagatt atgttatctc ctcatcccag atgaccggat
    16081 tacccggcca gaattacttt ttctttaccg gacgacgcca gccttctttc tgagtctgcg
    16141 gcacacggct gatagctaat gcattttcgc cgacattacg cgtcacagtt gtacccgcag
    16201 caatggtcgc gcctttgcct actgttaccg gggccaccag ctgagtgtcg gaaccgacaa
    16261 acacatcgtc gccgataatg gtcttaaatt tattcgcacc atcgtagttg caggtaatgg
    16321 ttcccgcgcc gatgttaacg ttatcgccaa tttccgcatc gcccaggtaa gtcagatgac
    16381 cagctttcga gcctttaccc agacgcgctt ttttcatctc aacaaagtta ccgacgtgtg
    16441 caccttccag caactcagca ccaggacgca gacgggcaaa cgggccaata gtacaggccg
    16501 ctgccagatt cacgtcttcc acaacggtat acggactgat ttcgcaatca tcgccaatca
    16561 cgctgttttt aatcacgcaa ccggtgccga ttttcacgcg atgaccgaga gtcacgttgc
    16621 cctcgatgat aacgttagta tcaatttcaa catcgcgccc gtgagtaagc gtaccgcgca
    16681 gatcaaaacg cgccggatcg cgcagcataa cgcctgctaa cagcagtttt tcagcctgtt
    16741 cggactggta aacgcgctcc agacgggaga gttgcaggcg gttattcaca ccttctactt
    16801 cgcttaaacg ttgcggatga acggcgacga tttcacgccc ttcctgatac gccagcgcaa
    16861 taatgtcggt gatgtagtat tcgccctgag cattattgtt ggtcagcttc gccagccagc
    16921 gtttcatatc tgcgccgtta gagatcagaa tgccggtgtt gatctcctga atctgacgct
    16981 gctcgtcggt ggcatctttg tgctcaacaa tgccggtaac tttgccattt tcacgggtga
    17041 tacgtccata accggtcgga tcatccagtt tcaccgtcag cagaccaatg ccaccctgcg
    17101 gtttagcatc acgcaggcgc tggagtgttt cgacagagat cagcggcacg tcgccgtaga
    17161 gcattaaaat gtcttcatca tcggcaaaga aaggtgcggc ctgctgcatt gcatgacccg
    17221 tacccagctg ctctgcctga agcacccagt taaggttgtc gtctttcagc gcctgtttaa
    17281 gcagatcgcc gccgtgaccg tacaccaggt gaacgtgcgc tgcgcctaat tcattcgcag
    17341 catcaatgac atgctgaacc atcgctttcc cggcaagggt atgcagcact ttcggaagat
    17401 cggaatacat gcgcgtgcct ttgcctgcgg caaggatcac tacgctcata gcattattca
    17461 acatacgcgt cctgactgta atttgagaac gaatttaaac cgcttcacct tgaaaaaact
    17521 acattttttt catcgtgaaa tggacagagg ataaattgtt caataacgga ttatccccgc
    17581 tgacgaaagt gccattttcg accatcgtca cgccttttgt ctgcagaaaa ttaaggcagt
    17641 accttcattt aagcgtcagg tggatgtttt tgctcttatt tcgatcaatg aataaacggg
    17701 aaaacagggg tgttttatat cagcaggatc tatgtgaacg ctcttcagga cggatcacgc
    17761 gtaaaaaaaa accagcctgt ttccagactg gcttttgtgc ttttcaagcc ggtgttacat
    17821 cgcttttttg gtcaactcga taacgcgcag ctgcgcgatc gctttggcca gttccgcaga
    17881 cgcctgagcg taatctacgt cgccgtgaga gctgctaatg tgctcttcag ccttacgttt
    17941 cgcttccatg gctcgcgctt cgtcgagatc ctggccgcga attgcggtgt cggccagaac
    18001 ggtcacgttg ccagactgca cttcaagaat gccgccagac agatagataa actcttcgtg
    18061 accgtgctgt ttcacgatgc gaatcatacc aggcttaatg gcggtgagca gcggtgcgtg
    18121 gccagggtag atccccagtt caccttcgct acccgttacc tggattttct cgaccagacc
    18181 agagaacatt tgttgctctg cgctgacgac gtccaggtgg taagtcattg ccatatcacc
    18241 ctccgattaa ggcgttaaag ttttttggct ttttccacag cttcttcgat ggaaccgacc
    18301 atgtagaacg cctgctccgg caggtgatcg tattcgcctt ccatgatgcc tttaaagcca
    18361 cggatggtgt ctttcaggga gacgtattta cccggagaac cggtgaatac ttctgccacg
    18421 aagaacggct gggacaggaa gcgctggatc ttacgagcac gcgctaccac cagtttgtct
    18481 tcttcagaca gttcatccat acccaggatg gcgatgatgt ctttcagttc ctgataacgt
    18541 tgcaggatgg actgaacgcc acgcgcggtg tcgtagtgtt cctgaccaac caccagcggg
    18601 tccagctgac ggctggtgga gtccagcggg tcaacggccg ggtagatacc cagagacgcg
    18661 atctgacggc tcagtaccac ggttgcgtca aggtgcgcaa aggtggttgc cggagacggg
    18721 tcagtcaagt catccgcagg tacgtatacc gcctgtacgg aggtgataga accagttttg
    18781 gtggaggtga tacgttcctg cagaacgccc atctcttccg ccagggtcgg ctgataacct
    18841 accgctgaag gcatacggcc cagcagtgcg gatacttccg taccggccag ggtgtaacga
    18901 tagatgttgt caacgaacag cagaacgtca cgaccttcgt cacggaattt ctcagccatg
    18961 gtcagaccgg tcagagcaac gcgcagacgg tttcccggcg gctcgttcat ctggccatac
    19021 accagggata ctttgtcgat aacgttggag tcggtcattt cgtggtagaa gtcgttaccc
    19081 tcacgagtac gttcacctac gcccgcaaac acagagtaac cggagtgctc gatcgcgatg
    19141 ttacgaatga gctccatcat gtttacggtt ttacctacac ccgcaccacc gaacagacca
    19201 actttaccgc ccttagcgaa cggacacatc aggtcgataa ctttgatacc ggtttccagc
    19261 agttcctgag agtttgacag ctcttcgtag gaaggtgctg cgcggtgaat cgcccaacgc
    19321 tcttcttcac cgatctcgcc tttcatgtcg accggttcac ccagtacgtt catgatacgg
    19381 cccagagtcg ctttacctac cgggacttcg atcgggtgtt cgaggtcttt tacatccaga
    19441 ccgcgacgca gaccgtcgga ggaacccatt gcgatggtac gcacgatacc gccgccgagc
    19501 tgctgctgaa cttccagcac cagacgctca ttaccatttt gcacctcaag agcatcgtac
    19561 acgcgcggta cggcatcctg agggaattcg acgtcaacta cggcgccgat tacctggaca
    19621 atctttccag tagccatctt aaatcctcta cgaaataacc tgtttaaacc gcggcggccc
    19681 ccgagacgat ctcggtgagt tcctgagtaa tgctggcctg acgagctttg ttgtatacca
    19741 actgcagctc tttaatcagg ctgccgccat tgtcggtcgc ggctttcatc gccaccatac
    19801 gggcggcctg ctcgctggcc aggttttcaa ccacgccctg ataaacctga gattcgacat
    19861 aacgacgcag cagggtatcc agcaacgcct tcggatcggg ttcgtacagg taatcccagg
    19921 atttatgttt cagatcatca tcatctgatg ccggtaacgg cagcagctgg ctgatggtcg
    19981 gaacctgaga catggtgtta ataaatttgt tgctgacaat gtaaagcttg tccagacggc
    20041 cttcgtcgta ggcctgcaac atcactttta ccggaccgat cagttcggac agggaagggt
    20101 tatcccccat gccggtgacc tgggcaacaa cattgccgcc cacggagttg aagaacgaca
    20161 cgcctttcga gccgatcatt gcgaggtcgc attgaacgcc tttgtcggtc caggtcttca
    20221 tttccgccag cagttttttg aacaggttaa tgttcaaacc accgcacaaa ccacggtcgg
    20281 tcgacaccac caggtagccc acgcgtttaa cgtcgcggtc ttccaggtaa aggtgcttat
    20341 attccagatt accatgtgca aggtgaccaa tcactttgcg catggtttct gcataaggac
    20401 ggctggccgc catgcgatcc tgcgatttac gcattttgga agcggcgacc atctccatcg
    20461 ctttagtgat cttttgcgtg ttctggacgc ttgcgatctt actacgtatc tcttttgcgc
    20521 cggccatgag cttctcctca atgccttgcg gcctgcccta aggcaagccg ccagacgtta
    20581 ccaggattgg gttgctttga aggaatcgag gatgcctttc agtttgcctt cgatttcgtc
    20641 gttgtagcca ccggtctggt tgatctcttg catcaacgga gcgtgatcac ggtcgacgta
    20701 agccagcaga gcggcttcga agctgccaat tttcgacagt tcaacatccg ccaggtaacc
    20761 acgttctgct gcgaacagaa ccagagactg ctgcgcaacg gacatcggcg catactgttt
    20821 ctgtttcagc agttcggtca ctttctgacc gtggtcaagc tgcttacgtg ttgcatcgtc
    20881 aaggtcggat gcaaactgag agaacgctgc cagttcacga tactgtgcca gagcggtacg
    20941 gataccaccg gacagttttt tcatgatctt ggtctgtgct gcaccaccaa cacgggatac
    21001 ggaaataccc gggttaaccg caggacgaat accggcgttg aacaggttgg tttccaggaa
    21061 gatctgacca tcggtaatgg agattacgtt ggtcggaacg aacgcagaaa cgtcacccgc
    21121 ctgagtttcg ataatcggca gtgcggtcag agaaccggtt ttccctttca cttcaccttt
    21181 ggtgaaggct tcaacgtatt cggcgttaac acgtgcagca cgctccagca gacgagagtg
    21241 gaggtagaaa acgtcgcccg ggaatgcttc acgtcctggc ggacgacgga gcagcaggga
    21301 gatctgacgg taagcaacag cctgtttaga caggtcatcg taaatgatca gcgcatcttc
    21361 accgcggtca cggaagtatt cgcccattgc gcaaccggca tacggtgcca ggtattgcag
    21421 tgcagcggat tcagacgcgg ttgctaccac aacgatggtg ttagccagtg cgccgtgctc
    21481 ttccagttta cgtaccacgt tagaaatggt ggacgctttc tggccgatag cgacatagat
    21541 acatttgata ccggaatcgc gctggttgat gatggcatcg atagccagtg cggttttacc
    21601 tgtctgacgg tcaccgatga tcaattcacg ctgaccacga ccgattggga tcatggagtc
    21661 aacggcttta taaccggtct gtaccggctg atctacggac tgacgttcga taacgcccgg
    21721 agcgattgct tctacagcag agaagccgtc gtgatccagc ggacctttac cgtcgattgg
    21781 tgcacccaga gtgttaacca cacggcccag caggccacgg ccaaccggaa cttccaggat
    21841 acggccagta cacttaactt tcatgccttc ggcaaggtca gcgtacggac ccataacaac
    21901 cgcacctaca gagtcgcgct cgaggttcag tgcgatagcg taacggttac ccggcaggga
    21961 gatcatttca ccctgcatac aatcggccag gccgtgaatg cggataacac cgtcacttac
    22021 agaaacaata gtaccttcgt tgtgagcttc actcacaaca ttgaactgag caatgcgctg
    22081 cttgatcagt tcgctgattt cggtggaatt cagttgcatg ctccagtccc cttaagactg
    22141 caagacgtct gcaaggcgct caagacgacc gcgtacgctg ccatcaatga ccatatcacc
    22201 cgctcggatg ataacgcctg ccattacaga cttatcgatt ttgcaattca gcttaacttt
    22261 gcgtgacaga cgtttttcca tcgcagcaga aattttcgcg agctgttgtt cactcagtgc
    22321 ggcagcggaa atgacgtcta cctcagcggt agcctcactc acggcacgaa ggtgaataaa
    22381 ctgctccaga acatccggga gcgcgttaag acgaccattt tcagccatta cccgaatcag
    22441 gttctgaccg ttttcgtcca gttgctcacc acaaactgcg ataaacgact cggcgagcgt
    22501 ttctggcgca agcgcgccag agagaagctc tgccatttgt tcgtttttgg ttacctcggc
    22561 ggcaaacgcc agcatgtcct gccagcgttc tacactttgg tgttcgacgg caaagtcaaa
    22621 agctgctttg gcgtaggggc gagctaccgt aataaattca gacatcagcc cctccctcct
    22681 tacagttcag cgacaagttt atccacgatg tcgctgttag cagcttcatc cacggaacgt
    22741 tcgatgatct tctcggcgcc agcaacagcc aggatagcaa cttgcttacg cagctcttca
    22801 cgggcacgtt tacgctcggc ttcaatttct gcctgcgcct gggccacgat tttagtacgt
    22861 tcctgttctg cctcagcttt cgcttcgtcc agaatctgcg agcggcgttt gttcgcctgc
    22921 tcgatgatta cctgggcttc cgctttcgct tttttcagct ggtcggtcgc gctggccttt
    22981 gcaaggtcaa ggtccttatg tgctcgttct gcggaagcaa ggccgtcagc aatttctttt
    23041 tgacgttttt cgatggctgc cattaatggc ggccatacgt acttcatgca gaacagaacg
    23101 aacaggacaa acgcgatggc ctggccgagg attgttgcgt taagattcac agcacaatgc
    23161 ctctatttag ttaacgttct gatattgctc tttaaataaa agcaacgctt actacgcgac
    23221 agcgaacatc acgtacagac ccagacctac agcgatcatc gggatagcat ccaccagacc
    23281 cataacgata aagaactgag tacgcagcag aggaatcaga tcaggttgac gcgctgcgcc
    23341 ttccaagaat ttacccccga ggatgccgat accgatcgca gcaccgattg ccgccagacc
    23401 catcatcaca gcggcagcca tgtacagcag atccatattc aggttttcca tgacagtctc
    23461 cagtttgttt cagttaaaac gtagtagtgt tggtaaatta atgttcttca gacgccatcg
    23521 acagatagac gatcgtcaga accatgaaga tgaaggcttg cagcgtaatg atcaggatgt
    23581 ggaaaatggc ccacggcaca ttcaggatcc actgtgacca ccacggcaac agaccagcaa
    23641 tcagaatgaa aatcagctca ccggcataca tgttaccgaa cagtcgcaaa ccgagtgaaa
    23701 ctggtttgga cagcaggctt accccttcaa ggattaagtt gacaggaatg aacgcccagt
    23761 gattgaacgg ctgcagcgtc aactctttcg tgaagccgcc gatgcctttc attttgatgc
    23821 tgtagaacag aatcaggata aatacgccca atgccataga cagcgttacg ttcacgtccg
    23881 cagacggaac cacacgcagt gcaggcagac ccagtacatg ttcagcaatg tacggcagca
    23941 ggtcgatagg cagtaaatcc atcaggttca tcaggaatac ccagacgaag atcgtcaggg
    24001 ccagcggagc aatcagcttg cttttgccat ggtacatgtc tttcacgcta ccattaacaa
    24061 agccgatcac cagctcaatc gcggtctgaa acttacctgg cacaccgctg gtcgcctttt
    24121 tggctacgct acggaataaa accaggaaca acagacccag caccaccgag aagaacatgg
    24181 agtcaatatt gattgtccag aaggtggctg gggggttttg tggatccacc agcgagaatg
    24241 tacgcaggtc cagctgaagg ttattcaggt ggtgtcctat gtaatcctgc ggcgtcatat
    24301 tttctgaagc catgatgcct tttacccttt gttgttaatt acagccggtg ccagtatctg
    24361 aaccaccagc accaaaaccc acgtaacgat cagcggcaag aataccgcct ttaaaaccgc
    24421 caacgccacc accagtaaca ccaacatcgc cagaactttg aaagcttcgc caaatgcgaa
    24481 tgtccaggcc acccggcctt tcgctggtgt atgcgcctgg tgacgccagg caaatatcat
    24541 aaacaaaacg ttaggcagaa agactgccag gcccccgctt attgcagaga cgccccagaa
    24601 ggggtctttg aggctgaaca gcaatccact tgctatcacc accagtaact gaacgagcag
    24661 aagcttccga gcaacgtttc gactcacgag cgacacagac atcacgtttt ttactcctgc
    24721 tcccttcgag gtatgccgcg tgtcgtataa aactttcttt aaggcttaga gtcaagcatc
    24781 aaaaagcggt caaattatac ggcgcgcccc cgtgatttca aacaataagt agccaaaagg
    24841 tgaataaatg tttaaatatt tttccagtgc atacaattgc gacttttcta ctaaccctgt
    24901 tcgatcatga aaaactgtaa ataacgcgta aacactggtg ataaagcgtg cttcagatca
    24961 catattgcgc atgttcgcgc acagcatatt tatttacttg gcaaatgatg cctttgcaag
    25021 tttatgatat ttcagtctaa aaacagatac tgttttaata aatgacattt acacaacaaa
    25081 aaccacccat tgacattttt aataatgttt tagcagccaa tgatggttct tagcgccgat
    25141 ttttagcaga ctgatatttt cactaatgac ttattttctg cttaccaaaa aaagccacgt
    25201 tatcttgttg atgcaaaaga gtgaacgtgg cgttaaatgt aaccagttat atcagtagaa
    25261 aacctggttg ttgttaacag tctaaccggt caatttttta tgattttttt gataaaaatt
    25321 aaattttatt tgctttaatc accaccagat gacgttcgcc atccagggct ggaacctgaa
    25381 gtttaaccac tgattcgacc tgatattctt cgggcaacaa agcgatttca tcttccggca
    25441 tttgcccttt cagcgcgtag aaacggcctt gctcaccagg aagatggtgg caccagctca
    25501 ccatatcgtt cagagaggca aaagcgcggc taattacgcc atcaaatggc ggctctgaag
    25561 gaaactcttc taccctgctc tgtacaggtt caatattctc cagtttaagc tcatgttgca
    25621 cctgacgaag gaaacgcacg cgtttaccaa ggctatccaa cagagtgaaa tgggcttcag
    25681 gacgcacgat agagagtgga atgcctggca gtcctggtcc ggtgccgaca tcgataaacc
    25741 gttcaccttg cagatacggt gccaccacaa tgctatcgag aatatggcgt accagcatct
    25801 cattaggatc gcggaccgaa gtcaggttgt acgctttgtt ccatttatgc agcatattca
    25861 cgtaggcaat aagctggttt ttctggtgat cggtaagcga aatacctgcg tctttcagca
    25921 gtaaggagag tttgttgagc acggtgatta cctgttcttg atgcgttgcc tggtaagcgg
    25981 gtgcttacca ggcattttta atgcgttatg cgctacgacg cagcataccc tgttttttca
    26041 gccacaccag cagaatggag atagccgcag gcgtgacgcc agaaatacgc gaagcttggc
    26101 cgatagaggc tggtttgtga tcgttaagtt tggcgatcac ttcgttagaa agaccggata
    26161 cctggcggta atccagtgtc gcgggtagca gggtgttctc gttacgcagc tgcttttcga
    26221 tctcatcttg ctggcgcgcg atataacctt cgtatttaac ctgaatctca acctgttccg
    26281 ccgcctgttc gtctgtcaac gcaggggcaa acggcgtcag cgtggttaat ttttcataag
    26341 tcatttccgg acgacgcagc agatcttcac cactggcttc acgggaaagc ggcgcagtca
    26401 ggtgagcatt cacttcggct gcagcttccg ccgacggggt tacccaggtc gatttcagac
    26461 gctgacgctc acgctcgata ttctcaagtt tctcgttaaa gcgcgcccaa cgttcgtcat
    26521 ccaccaggcc cagttcacga ccgatttcag tcaaacgcag atccgcatta tcttcgcgta
    26581 gcatcagacg atattctgcg cgcgaagtaa acatacgata cggttctttg gttcctaaag
    26641 tgcacaggtc atcaactagt acgccgagat acgcctgaga acgtgccgga gcccaacctt
    26701 ctttgtcagc agacagacgg gcagcgttaa gaccggccag caaaccttgc gcagcggctt
    26761 cttcgtaacc ggtagtgccg ttaatctgac cagcaaagaa cagcccctgg ataaacttgc
    26821 tctccagcgt cggtttcagg tcgcgaggat cgaagaagtc atactcaatg gcataacccg
    26881 gacgcacgat cttcgcgttt tccatcccct gcatagagcg gacgatttgc atctgcacat
    26941 cgaacggcag gctggtggag ataccgttcg gataaatttc attagaggtc agtccttccg
    27001 gttcaaggaa gatctgatgc tgatttctgt cggcgaagcg catgactttg tcttcgatcg
    27061 acgggcagta gcgtgggccg acaccttcga tcacccctgc atacattggg ctacgatcga
    27121 ggttactgcg gatcacatca tgggttttct cgttggtatg agtgatataa cacggcacct
    27181 gctggggatg ctgggacgca ttgcccataa acgagaatac cggcattggg ttatcgccat
    27241 gctgttgcgc cagcacacta aagtcgatag ttcgagcatc aatacgcggt ggtgtcccgg
    27301 ttttcagacg accaacgcgc agcggcagtt cacgcaaacg gcgagaaagc ggaatggacg
    27361 gcggatcacc agcacggcca ccgctgtaat tatccagacc gatatgaatt ttaccgtcga
    27421 ggaacgtccc aacggtgagc acgacggctt tggcacggaa cttcagtccc atttgggtaa
    27481 cagcaccgac cacgcgatcg ttttcgacaa taagatcttc aaccgcctgc tggaagatca
    27541 tcaggttcgg ttggttctcc agcgccgtac gtaccgcctg acggtagagc acacgatccg
    27601 cctgagctcg ggtagcgcga accgccggtc ctttgcttgc gtttagtatc ctaaactgga
    27661 tacccgcttg atcgatcgct ttcgccatca gaccgccgag tgcatccact tcttttacca
    27721 gatgtccctt cccaataccg ccgatcgccg ggttgcagct catctgcccc agagtgtcga
    27781 tattgtgtgt caaaagcaga gtctgttgac ccatacgcgc cgcggccatc gcggcctcgg
    27841 tgcctgcatg acccccgcca atgatgatga cgtcaaaagg atccggataa aacatggtga
    27901 ttgcctcgca taacgcggta tgaaaatgga ttgaagcccg ggccgtggat tctactcaac
    27961 tttgtcggct tgagaaagac ctgggatcct gggtattaaa aaggagatct atttatttag
    28021 agatctgttc tattgtgatc tcttattagg atcgcactgc cctgtggata acaaggatcc
    28081 ggcttttaag atcaacaacc tggaaaggat cattaactgt gaatgatcgg tgatcctgga
    28141 ccgtataagc tgggatcaga atgaggggtt atacacaact caaaaactga acaaccgttg
    28201 ttccttggat aactaccggt tgaaccaagc ttcctgacag agttatccac agtagaacgc
    28261 acgatctgta tacttatttg agtaaattaa tccacgatcc cagccattct tctgccggat
    28321 cttccggaat gtcgtgatca agaatgttga tcttcagtgt ttcgcctgtc tgttttgcac
    28381 cggaattttt gagttctgcc tcgagtttat cgatagcccc acaaaaggtg tcatattcac
    28441 gactaccaat accgattgcg ccaaagcgga ccgcagaaag atcgggcttc tgttcctgca
    28501 atgcttcata gaaaggagaa aggttgtccg gaatatctcc ggcaccgtgg gtggagctga
    28561 taatcagcca gatccccgag gcagataaat cttccaacag cggaccgtgc agcgtttcgg
    28621 tggtaaaacc cgcctcttcc agcttttcag ccaggtgttc tgctacatat tcggcaccgc
    28681 cgagggtgct gccgctgata agagtgatat ctgccataaa ccgccacctt tattaagagt
    28741 ggcgtattgt acgctgtgaa cgcgttggga tctacctgtg gaaaagtatg ggattaaaaa
    28801 agccgatcag ggcttgatag tacgcatgat cgggtgttgc aggacgatca atgtctcggt
    28861 ggactgaatt tcatcaattg tttggatctt gttgataagt acatgctgga gagcgtcgat
    28921 cgaacggcac attactttta taaagatgct gtagtggcct gttgtgtaat aggcttcagt
    28981 gacttcatca aggctttcca gctttgccag cgcggaaggg tagtctttgg cgctctttaa
    29041 tataatgccg ataaagcagc ctacgtcata accgagctgc ttcgggctga catcaatacg
    29101 cgccccggta atgatccccg cctgcttcat tttctctacc cgaacgtgaa tcgtccccgg
    29161 actgacgcca aattgtttcg ccagttcggc gtaagcggtg cgcgcattgc ccattaatgc
    29221 ttccaggatg ccacggtcca gattgtcgat cagataattt tccataggat tttcttatgc
    29281 agattgatga ttcattctat tttagccttc ttttttaatg aatcaaaagt atgttaggct
    29341 ttttattgaa tgattattgc atgtgtgtcg gtttttgttg cttaatcata agcaacagga
    29401 cgcaggagta taaaaaatga aaaccgctta cattgccaaa caacgccaaa ttagcttcgt
    29461 gaaatctcac ttttctcgtc aactggaaga acgtctgggg ctgatcgaag tccaggcacc
    29521 gattcttagc cgtgtggggg atggcacgca ggataacttg tcgggctgtg aaaaagcggt
    29581 gcaggtaaaa gtgaaagctc tgcctgatgc ccagttcgaa gtgattcatt cactggcgaa
    29641 gtggaaacgt cagaccttag ggcaacacga cttcagcgcg ggcgaagggc tgtacacgca
    29701 catgaaagcc cttcgccccg atgaagaccg tctttctccg ttgcactcgg tctatgtaga
    29761 ccagtgggac tgggaacgcg taatgggcga cggtgagcgt caattctcga ctctgaaaag
    29821 cacggtagag gcgatctggg cgggaattaa agcaaccgaa gctgcggtta gcgaagagtt
    29881 tggcctggca ccgttcctgc cggatcagat ccacttcgta cacagccagg agttactgtc
    29941 tcgttatctg gatctcgatg ccaaagggcg tgagcgggcg atagcgaaag atcttggcgc
    30001 ggtattcctt gtcgggattg gcggcaagct gagcgatggt catcgccacg acgtgcgcgc
    30061 accggattat gatgactgga gcaccccgtc agagctgggt catgcgggtc tgaacggcga
    30121 tattctggtg tggaaccctg tactggaaga tgcgtttgag ctttcttcca tggggatccg
    30181 cgtggatgcc gacacgctga agcatcagct ggcgctgacc ggtgatgaag atcgtctgca
    30241 gctggaatgg catcaggcgc tgctgcgcgg tgaaatgccg cagaccatcg gcggcggtat
    30301 cggccagtct cgtttgacta tgctgctgct gcaactgccg catatcggcc aggttcagtg
    30361 tggagtatgg ccagctgctg ttcgcgagag cgtcccttct ctgctgtaat aatttatcgc
    30421 cgccagcgtc tgagcaggcg gcttcgcatc ccggtatcaa agcgccagat atgatcgaaa
    30481 atgcgcatga tgccgggttt gccgtgtgcc gacatcgcca cggcatgaaa gcgatgctga
    30541 tgtacccgct gcagctcttt cactttactc gtcacgtcgt caggcaaccg ctgagcgata
    30601 aaatcagaaa tcaccaccgc atcggcatca aaccattccc tgctttgcaa gcgttccata
    30661 atggcgcgaa aacaactggc aagatcggtg ccgccacgaa actgctggct taaaaaacgg
    30721 attgcttgtt cgatgccttg tgggcctgaa agctcataac ggacgatctc ggtggaaaat
    30781 agcataatat agcagcgccg gttttctgcg agagcaatgc gcatcaaggc caggcagaac
    30841 gctttcgcac actgttcatt aaagccgccc attgagccgg aagtatccac acagacaata
    30901 aacggcccgc gcggctgttc atcgtaatct ttatgtacca ccggacgttc gatcactttt
    30961 tcacgccacg actcaccgtg caggcgatag gtgagcaact gtttttccac cagccgacgg
    31021 taaaactcat actccagttc cgttatccct agtgtcgcca gttctggcgg caggagacgt
    31081 aaaatatcat cgctttgttg cagaccatca acctgctcag gaaccgtcgc cggttcgcgc
    31141 accatggtgc ggaaggtttc catctgcgca tcgttgcgcg gtattgattt ggcttcccga
    31201 gaacgcccca gctgctctgc caggcgtttc agttccggct gttcgttaag aaattcaccg
    31261 tatttcacaa tcaactgata gtcgccacgt ttaagctggc cggcgctcat atcccacaga
    31321 cgaccagctg cggtattgtt atctgcgaga atcggttcaa gttgtccgct cagcgtcatg
    31381 cgttcctgaa cttcacttaa cagttgttcg cgttcttctt ctaatagctg ttgatttaac
    31441 gtcgttgctt gcacgatcag acttaaacgc cagcgctgga gaaaaagcgt gtgtaacgct
    31501 gaagttattg tgctgttagc atcaaccaac tgccgggctt gttctgccca tggggaattc
    31561 agacgatgca gtaagtccag gatctgtggt agctgcacaa taaactgcgg cgtggagagg
    31621 agctggcttt gctgatagca catcacctct tcggtgagtt ctggcgggac tcgggcatct
    31681 ttcagccgac tgcgcagcgc ctcactccag cggggaacat catcagtgat tgctgccttc
    31741 agtcgtggga atttttcaaa gaagaccgcc agctgcggtg aggccagcag tgcgatgatc
    31801 atctcttcga tcaatccctc ttcgctgacg gccagcatca cattaagcgt atccagcgtt
    31861 agcattgttg tgcctggcga atctgtgcac cgacatcctg taggctggct tcaatgcgac
    31921 ctaaccagtc gccagggata aacagacatt tttgctgttc gctgaataaa gcgtgttgct
    31981 tacgccagtc gctttcaagc tcttccagtt gttgttttat ttcacctggc agaccttcag
    32041 ccgatgaacc gggaagtgcc agcgtactgc cttgtaaact tacatcgcgt acaacaagat
    32101 gttgggtgct atcaacttcc agattcagtt tctgggcaaa gccgataccg ttcagtttgc
    32161 cgcgaatttc accaccttta ctcagccact gttccagcgc gctacgctca aaggagatat
    32221 gaaccacttc catatcatgc agttttaacg gtttttgcag cagcagagtc agagtggaag
    32281 cagtaacgtt aacagggagt tgatactgct gacgacggct aaaaatgccg cccagacgaa
    32341 ttaccgttaa ggctgtttta tcgctttgtt gctgctgaag ttgcaggtga cgttgcacaa
    32401 tcgcgcctag gcgggtcaac atcccttgct gttgccaggc gtgaccggtc atcaatacat
    32461 caatttgttg ttgtatcaaa ttcaggcttt gcgcgtcata ccacaggcaa tctttcagca
    32521 aaatgagatc taccggggca acagcactgc gaccgctaaa aaaggcgctg gcctgcaata
    32581 atcggatcgc ttttttccag cgacgatccg agacataagg cgcatccggt aatttatcca
    32641 gttgctggcg cagcataaaa atcagctcaa atacatgatc gggcagcgta atttcaccaa
    32701 tctctttctg ccagcgttca tattcttcat ctgtgacctg caaggcatca ggaaccggat
    32761 tgtcgttttc atcctgttga ctggtcagca tggagcggaa attcgcttta tcctgcactt
    32821 tatctaacca cagacgaatc agcatgcggt catataacgc ttccagactg ctgtctgctt
    32881 ccggcagctc gttggaggcc gccaccagca gacgcatcgg gattttttct acgtgtgcac
    32941 cgttgcggaa ctggcgctcg ttaatggcgg tgagcaaggt attaagaatt gccgggcccg
    33001 ctttccagat ctcatccaga aagacaattt ctgcttccgg caggtaaccg ctggttaaac
    33061 gttcatagcg cccttcatct tttagcgcct gaatagaaag gggaccaaaa acttcttccg
    33121 gcgtggagaa gcgggtcatc agatattcaa acgcgcgggc attctgaaag gcgaatttta
    33181 agcgccgggc gatcaaactt ttggcaatac ctggcgggcc aaggaggaac acactttcac
    33241 cacttaatgc cgctaataaa cacaagcgga tggcgtggct acgttcataa agcccctttt
    33301 ccaacgaact gctcaggcgg gaaattcttt ccgctaataa atgagggtga gccataatga
    33361 agtggcgtcc tttcgtcaaa agttctgcgt aaattgcgag tatagacgtt tcttgctggt
    33421 ggctaaaata gtctcaaagg ggggtaatgc cagtcagtta agcaactgac tgg
//
LOCUS       DAZPEB010000022        32957 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZPEB010000022 DAZPEB010000000
VERSION     DAZPEB010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32957
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.022"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..256
                     /locus_tag="ACHURJ_000944"
     CDS             <1..256
                     /locus_tag="ACHURJ_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFR1279993.1"
                     /translation="LDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNGLSVV
                     MTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            266..1640
                     /gene="gspE"
                     /locus_tag="ACHURJ_000945"
                     /pseudo
     CDS             266..1640
                     /gene="gspE"
                     /locus_tag="ACHURJ_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414649.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
     gene            1630..2832
                     /gene="hofC"
                     /locus_tag="ACHURJ_000946"
     CDS             1630..2832
                     /gene="hofC"
                     /locus_tag="ACHURJ_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFR1279994.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAGQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSW
                     LLVLFGFLLAIANKLLMRRPTWLIVRQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNTGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(2867..3910)
                     /gene="guaC"
                     /locus_tag="ACHURJ_000947"
     CDS             complement(2867..3910)
                     /gene="guaC"
                     /locus_tag="ACHURJ_000947"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFR1279995.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSDQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(4066..4110)
                     /locus_tag="ACHURJ_000948"
     CDS             complement(4066..4110)
                     /locus_tag="ACHURJ_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1279996.1"
                     /translation="MIIKTLKMSARKRN"
     gene            4135..4755
                     /gene="coaE"
                     /locus_tag="ACHURJ_000949"
     CDS             4135..4755
                     /gene="coaE"
                     /locus_tag="ACHURJ_000949"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFR1279997.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            4755..5498
                     /gene="zapD"
                     /locus_tag="ACHURJ_000950"
     CDS             4755..5498
                     /gene="zapD"
                     /locus_tag="ACHURJ_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFR1279998.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDSENGQVPERLDFELACC"
     gene            5508..5705
                     /gene="yacG"
                     /locus_tag="ACHURJ_000951"
     CDS             5508..5705
                     /gene="yacG"
                     /locus_tag="ACHURJ_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFR1279999.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(5812..6201)
                     /gene="mutT"
                     /locus_tag="ACHURJ_000952"
     CDS             complement(5812..6201)
                     /gene="mutT"
                     /locus_tag="ACHURJ_000952"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFR1280000.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLAYEFPDRHITLWFWLVESWEGVPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(6261..8966)
                     /gene="secA"
                     /locus_tag="ACHURJ_000953"
     CDS             complement(6261..8966)
                     /gene="secA"
                     /locus_tag="ACHURJ_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFR1280001.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(9028..9540)
                     /gene="secM"
                     /locus_tag="ACHURJ_000954"
     CDS             complement(9028..9540)
                     /gene="secM"
                     /locus_tag="ACHURJ_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFR1280002.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNVPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQYAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(9771..10688)
                     /gene="lpxC"
                     /locus_tag="ACHURJ_000955"
     CDS             complement(9771..10688)
                     /gene="lpxC"
                     /locus_tag="ACHURJ_000955"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFR1280003.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSCNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFIAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLTFKAPSAVLA"
     gene            complement(10789..11940)
                     /gene="ftsZ"
                     /locus_tag="ACHURJ_000956"
     CDS             complement(10789..11940)
                     /gene="ftsZ"
                     /locus_tag="ACHURJ_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFR1280004.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(12001..13263)
                     /gene="ftsA"
                     /locus_tag="ACHURJ_000957"
     CDS             complement(12001..13263)
                     /gene="ftsA"
                     /locus_tag="ACHURJ_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFR1280005.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(13260..14090)
                     /gene="ftsQ"
                     /locus_tag="ACHURJ_000958"
     CDS             complement(13260..14090)
                     /gene="ftsQ"
                     /locus_tag="ACHURJ_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFR1280006.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKETAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQLAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(14092..15012)
                     /gene="ddlB"
                     /locus_tag="ACHURJ_000959"
     CDS             complement(14092..15012)
                     /gene="ddlB"
                     /locus_tag="ACHURJ_000959"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFR1280007.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLDASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(15005..16480)
                     /gene="murC"
                     /locus_tag="ACHURJ_000960"
     CDS             complement(15005..16480)
                     /gene="murC"
                     /locus_tag="ACHURJ_000960"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFR1280008.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFLGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(16534..17601)
                     /gene="murG"
                     /locus_tag="ACHURJ_000961"
     CDS             complement(16534..17601)
                     /gene="murG"
                     /locus_tag="ACHURJ_000961"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFR1280009.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(17598..18842)
                     /gene="ftsW"
                     /locus_tag="ACHURJ_000962"
     CDS             complement(17598..18842)
                     /gene="ftsW"
                     /locus_tag="ACHURJ_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFR1280010.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVVFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(18842..20158)
                     /gene="murD"
                     /locus_tag="ACHURJ_000963"
     CDS             complement(18842..20158)
                     /gene="murD"
                     /locus_tag="ACHURJ_000963"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFR1280011.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(20161..21243)
                     /gene="mraY"
                     /locus_tag="ACHURJ_000964"
     CDS             complement(20161..21243)
                     /gene="mraY"
                     /locus_tag="ACHURJ_000964"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFR1280012.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(21237..22595)
                     /gene="murF"
                     /locus_tag="ACHURJ_000965"
     CDS             complement(21237..22595)
                     /gene="murF"
                     /locus_tag="ACHURJ_000965"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFR1280013.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(22592..24079)
                     /gene="murE"
                     /locus_tag="ACHURJ_000966"
     CDS             complement(22592..24079)
                     /gene="murE"
                     /locus_tag="ACHURJ_000966"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFR1280014.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVEQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKV
                     IDVNYHDSGVTIRFSSSWGDGEIESHLIGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTVPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(24066..25832)
                     /gene="ftsI"
                     /locus_tag="ACHURJ_000967"
     CDS             complement(24066..25832)
                     /gene="ftsI"
                     /locus_tag="ACHURJ_000967"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFR1280015.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(25848..26213)
                     /gene="ftsL"
                     /locus_tag="ACHURJ_000968"
     CDS             complement(25848..26213)
                     /gene="ftsL"
                     /locus_tag="ACHURJ_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFR1280016.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(26210..27151)
                     /gene="rsmH"
                     /locus_tag="ACHURJ_000969"
     CDS             complement(26210..27151)
                     /gene="rsmH"
                     /locus_tag="ACHURJ_000969"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFR1280017.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(27153..27611)
                     /gene="mraZ"
                     /locus_tag="ACHURJ_000970"
     CDS             complement(27153..27611)
                     /gene="mraZ"
                     /locus_tag="ACHURJ_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFR1280018.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(28213..29217)
                     /gene="cra"
                     /locus_tag="ACHURJ_000971"
     CDS             complement(28213..29217)
                     /gene="cra"
                     /locus_tag="ACHURJ_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFR1280019.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANAPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHLMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(29299..29385)
                     /locus_tag="ACHURJ_000972"
     CDS             complement(29299..29385)
                     /locus_tag="ACHURJ_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFR1280020.1"
                     /translation="MCNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(29397..29888)
                     /gene="ilvN"
                     /locus_tag="ACHURJ_000973"
     CDS             complement(29397..29888)
                     /gene="ilvN"
                     /locus_tag="ACHURJ_000973"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFR1280021.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIREVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(29891..31615)
                     /gene="ilvI"
                     /locus_tag="ACHURJ_000974"
     CDS             complement(29891..31615)
                     /gene="ilvI"
                     /locus_tag="ACHURJ_000974"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFR1280022.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVVAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            31734..31778
                     /locus_tag="ACHURJ_000975"
     CDS             31734..31778
                     /locus_tag="ACHURJ_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1280023.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(31933..32877)
                     /gene="leuO"
                     /locus_tag="ACHURJ_000976"
     CDS             complement(31933..32877)
                     /gene="leuO"
                     /locus_tag="ACHURJ_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFR1280024.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT         7912 a         9255 c         8018 g         7772 t
ORIGIN      
        1 attagatacc tgcgacggtg gcagcaatgg cattccctcg cctaccacca cccgctatgt
       61 ttcagccatg agtgtggcaa aaggcgtggt gtcgctgacc gggcaagaaa gtctcaatgg
      121 actaagcgtc gtcatgacgc caggttggga taacgcaaac ggcgtcaccg gctgggcgcg
      181 caactgcaat attcaaagtg acagcgcatt gcagcaagcc tgcgaagatg tcttccgctt
      241 tgatgacgcc aactaaggag cggcaatgaa tattccacag ctcaccgccc tgtgtctgcg
      301 ttatcaggga gtcttgctgg atgccagcga agaggtggtt catgttgcgg tagtcgatgc
      361 accttcgcat gagttgctgg acgcattgca tttcgctacc accaaacgta ttgagatcac
      421 ctgctggacg cgccaacaaa tggaaggcca cgccagtcgc acacaacaga cattgcccgt
      481 agctgttcag gagaagcatc agtcgaaagc agagttgctg actcgaacgt tacaatctgc
      541 gctggaacaa cgtgcgtctg atattcatat cgaaccagcg gacaatgcct accgcatccg
      601 cttgcgtatc gacggcgtat tgcatccttt accggatgtt tcaccggatg ccggagtcgc
      661 attaaccgcc agattaaaag tgctgggaaa cctggatatt gcggaacatc gcctgccgca
      721 ggacgggcaa ttcactgtcg aactggcagg aaacgccgtc tcatttcgta ttgcgacctt
      781 accatgtcgg ggtggtgaaa aggtggtatt aaggttgtta cagcaggtga accaggcact
      841 ggatgttaac acgctgggaa tgcagccgtt acaactggcg gactttgctc atgccttgca
      901 acaaccacag ggactggtgc tggtaactgg ccctacaggc agcggcaaaa cggtcacgct
      961 ttatagtgcc ctgcaaaagc tgaataccgc tgacattaat atttgtagcg tcgaagatcc
     1021 ggttgagatc cccatagccg gactaaacca gacgcaaatc catccgcgtg ccgggctcac
     1081 ctttcagggc gttttgcgtg cgttattgcg ccaggatcct gacgtcatca tgatccgatg
     1141 gcgaaacggc agaaattgcc attaaagccg cgcaaaccgg tcacctggtg ttgtctaccc
     1201 tacacactaa ttccacctgc gaaacgctgg tacgtttaca gcaaatggga gtcgcccgct
     1261 ggatgctctc atcagcgctt acgctggtaa tagcccagcg tctggtacgt aaattttgcc
     1321 cacattgtcg ccagcagcaa ggggagccca tccatattcc agacaatgta tggccgtcgc
     1381 cgctgcccca ctggcaggca cccggttgtg tacattgcta ccacggtttt tatggtcgta
     1441 ccgccttgtt tgaagtcctg cccattacac cagccatacg ccagcttatt tccgctaata
     1501 ccgacgttga atcgctggaa acgcacgcac gacaggcggg tatgcgtacg ctttttgaaa
     1561 acggctgcct agccgtggag caaggcttaa ccacctttga agagttaatc cgcgtactgg
     1621 ggatgccgca tggcgagtaa gcaactctgg cgctggcatg gcataaccgg cgacggcaat
     1681 gcgcaagatg ggatgctatg ggcagagagc cgtgctttgc tgctcatggc actacagcaa
     1741 cagatggtta ccccacttag cctgaagcga atcgccatca attctgcgca gtggcgagga
     1801 gataaaagcg cggaagtcat tcatcaactg gcgacgctac tcaaagccgg gttaacgctt
     1861 tctgaagggc tggcactgct ggcgggacag catcccagta agcaatggca agcgttgctg
     1921 caatcgctgg cgcacgatct cgaacagggc attgcttttt ccaatgcctt attaccctgg
     1981 tcagaggtat ttccgccact ctatcaggcg atgatccgca cgggtgaact gaccggtaag
     2041 ctggatgaat gctgctttga actggcgcgt cagcaaaaag cccagcgtca gttgaccgac
     2101 aaagtgaaat cagcgttacg ttatcccatc atcattttag cgatggcaat catggtggtt
     2161 gtggcaatgc tgcattttgt tctgccggag tttgccgcta tctataagac cttcaacacc
     2221 ccactaccgg cactaacgca ggggatcatg acgctggcag actttagtgg cgaatggagc
     2281 tggctgctgg tgttgttcgg ctttctgctg gcgatagcca ataagttgct gatgcgccga
     2341 ccgacctggc ttatagtgcg gcagaaattg ctgttacgca tcccgattat gggttcactg
     2401 atgcggggac aaaaactcac gcagatcttt acgattctgg cgctgacaca aagtgcaggc
     2461 attacttttt tgcagggcgt agagagcgtc agagaaacaa tgcgctgccc gtactgggtg
     2521 caacttctga cacaaatcca gcacgatatc agtaacggtc atcccatctg gctggcgcta
     2581 aaaaataccg gggagtttag cccgctctgt ttgcaattag tgagaacagg agaggcatcc
     2641 ggctcgctgg acctcatgtt agacaacctc gcccatcatc atcgggataa cacaatggcg
     2701 ctggcggata acctcgcagc cttactggaa ccggcgttgc tgatcataac gggaggaatt
     2761 atcggtacgc tggtggtggc gatgtatctg ccaattttcc atttaggcga tgcgatgagt
     2821 gggatgggat aacgctggcg tgttgctcca caccagcgtt ggaagattac aggttgttga
     2881 agatgcggtt ttcttgttcc tgcacacgga taaatgtggt gcgcttggtc agttctttca
     2941 ggcgtgaagc cccaacgtat gtacaagctg aacgcaggcc gcccaaaata tcacgcgcgg
     3001 tattttcaac cgggcctcgc agcggcagct taacggtttt accttctgct gcgcgatatt
     3061 ccgcaacgcc gccaacgtga cgtttcatcg cagactcgga gctcatgccg tagaacagca
     3121 taaatttctc gccgttctcc tcaacgatgc gaccgccgct ctcttcgtgg cccgccagca
     3181 tgccgccaag catgacgaaa tcggcaccgc cgccaaaggc ttttgcgaca tcgcccggcg
     3241 tggtgcagcc accgtcgctg acgatcattc cgcccagacc gtgcgccgca tcggcacatt
     3301 cgattaccgc agaaagctgc ggataaccga cgcctgtttt gacgcgagtt gtacaaacag
     3361 aacctgggcc aatgccaact ttaacgatat cagcaccgga gaggataagc tcctcacaca
     3421 tttcaccagt cactacgttg ccagcacaaa tggttttggt cggccacgct tcacgcgctt
     3481 tcgcaacgaa ctgcacgaag tgttcggaat aaccattcgc cacatcaata caaacaaagt
     3541 ttaatgccgg gttcaggtcg agaatctgtt tagttttttc gaaatccgca tcagacgtac
     3601 cggtagaaac catcacatgt ttcagcacat cagcggaaga attgttgata aacgcttgcc
     3661 actcttcgac agaatagtgt ttatgcacag cagtcaaaat atcaaaagaa gccagcgcag
     3721 aggccataga aaatgtacct acggtgtcca tatttgcggc gataatcggc acgccggacc
     3781 agctctgatc tgaatgtttg aaggtgaatt gacgttccag ttcaacatcg gaacggcttt
     3841 taagagtgga gcgtttaggg cggatgagaa cgtctttaaa acctaacttc agatcttctt
     3901 caatacgcat gtgcggattc ctggggttaa tggctataag ggtaagagcg caactccagt
     3961 gacgctatca tacgcactaa tcaatgcggc gcaagactgc gaaattgtct ttttttacgc
     4021 tacaatccca taaatttacc tggtaaatag tgagcgaacg tttcatcaat ttcgctttcg
     4081 tgcactcatt tttaacgttt tgataatcat gatgaattcc cgggaatata aattatgagg
     4141 tatatagttg ccttaacggg aggcattggc agtggcaaga gtaccgttgc caatgcgttt
     4201 gctgatctcg gaattaacgt cattgatgcc gatattattg cgcgtcaggt ggttgaacca
     4261 ggtgcacctg cgctacatgc cattgctgat cactttggcg ctaacatgat tgctgctgat
     4321 ggaacattgc agcgccgggc cttgcgcgag cggatcttcg ccaacccgga agagaaaaac
     4381 tggcttaacg ccctgctgca tccgctgatt cagcaagaga cgcaacacca gatccagcaa
     4441 gctacttccc cctatgtact gtgggttgtg ccattgctgg tagaaaactc actgtataaa
     4501 aaagcgaatc gagtgctggt ggtggatgtc agcccagaaa cgcaacttaa gcgcaccatg
     4561 cagcgcgatg atgtaactcg cgagcatgtc gaacaaatcc ttgctgctca ggcaacgcgc
     4621 gaagcccgcc ttgccgtggc agatgacgtc attgataata acggcgcacc ggatgctatc
     4681 gcatcggatg ttgcccgcct gcacgcacac tatttgcagc ttgcgtcgca gtttgtctca
     4741 caggaaaaac cgtaatgcag acccaggtcc tttttgaaca tccactaaat gaaaaaatgc
     4801 gtacatggct gcgcattgag tttttgattc agcaactcac cgttaattta cccatcgttg
     4861 accacgctgg cgcgctgcat ttcttccgta atgtcagtga attactggat gttttcgagc
     4921 gcggcgaagt ccgcactgag ctgttgaaag aacttgaccg gcagcaacgt aaactccaga
     4981 cctggattgg cgtgcctggc gtggaccaga gccgtattga agcattaatt cagcagttaa
     5041 aagcggcggg gagcgtatta atttccgcgc cgcgtatcgg gcaatttctg cgtgaagatc
     5101 gtttgattgc tctggtgcgt cagcgactga gcatcccagg cggctgttgc agctttgatt
     5161 tacctacatt gcacatttgg ctgcatctac cccaggcgca gcgcgacagc caggtagaaa
     5221 cctggattgc cagcctgaac ccgctcaccc aggcacttac catggtgctg gatttaattc
     5281 gccagtcggc ccccttccgt aaacaaacca gcctgaatgg tttttatcag gataacggtg
     5341 gcgatgccga cttgctgcgc ctgaatctgt cgctcgattc acagctttat ccgcaaattt
     5401 ccggacataa gagccgtttt gccattcgtt ttatgccgct ggacagtgaa aacggacagg
     5461 taccggaacg tctagatttc gaactggcct gttgctaagg agtgaaaatg tcagaaacta
     5521 ttacggtgaa ttgcccaacc tgcgggaaaa cggtggtatg gggtgaaatc agcccgtttc
     5581 ggccattttg ctccaaacgt tgtcagctga tcgacctcgg agaatgggct gctgaagaaa
     5641 aacgaatccc cagcagtggc gatctttccg aaagcgatga ctggagcgaa gagccaaagc
     5701 agtgacattg gtctgatgcc gcacataggc ctgataagac gcggacagcg tcgcatcagg
     5761 catcttgtgc aaatgtcgga tgcggcgtga acgccttatc cgacctacag actacagacg
     5821 cttaagcttc gcaattaccg gttcattggc tggcggaaaa tcatcggcat taagaccgac
     5881 cagcgacatc cactcaccgg gttgcccttc tttaccccac ggcacccctt cccaactttc
     5941 gaccagccag aaccacagag ttatatgcct gtccgggaat tcatatgcca gtttttcaaa
     6001 tagcgaaaaa tgttgtgggg taatcccgac ctcttcctga agttcacgca ccaccgcctg
     6061 ttccggcgtt tcgcccattt caattttacc gccgggaaac tccagtttat tcgccatgtg
     6121 cgcatctgct gcgcgacgcg ttataaagat ttcattgttc tcgttgcgaa taatacctac
     6181 cgcaatttgc agctttttca ttgttttaaa cctataaaaa aggcgcagaa tcctgcgcct
     6241 tttgcttcaa cagttagctt ttattgcagg cggccatgac actgcttgta ttttttacca
     6301 gaaccgcacg ggcaaggatc gttacgtcct actttgcgct caccggtttg cgccgccagt
     6361 gcagctgcgg ctgcagagtc gtcatcctga tggctaagct gctgcatttg cgctaaacgc
     6421 tcggcttcca tacgacgctg ttgttccagc tcctcaacct cttcaggcat acgtacctga
     6481 actttgctca gcgtactgat aacttcatat ttcaacgact ccagcatcgc tgcaaacatg
     6541 gagaacgatt cacgtttgta ttcctgcttc ggatctttct gtgcatagcc acgcaggtgg
     6601 ataccctgac gcagatagtc catcgctgcc aggtgctctt tccacaggga gtcaagcgtt
     6661 tgcagcatga cgcctttctc gaagtgacgc atcatctcag caccaaccac ttcttcttta
     6721 cgctgataca cttcgatgga ctgcgccaga atgcgctcac gcagcgtctc ttcatgcagt
     6781 tctggttctt tatccagcca ctcggcaatt ggtaaatcga ggtcgaaatc gttcttcaga
     6841 cgttcctgca gccccggaat atcccacatt tcttccagcg actgtggcgg aatgtaggca
     6901 tcaatggtgg ctttgaacac atcttcacga atgctgttga tggtttcgct cacatcgctg
     6961 acatccagca gttcgttacg ctgggagtaa atggcgcgac gctgatcgtt agccacgtca
     7021 tcatattcca gcagttgctt acgaatgtcg aagttacgac tttcaacttt acgctgggcg
     7081 ttggcaatcg ctttagtcac ccacgggtgt tcaatggctt cgcctggctt catacccagt
     7141 ttacgcatca tgccggatac tcggtcggaa gcaaaaatac gcatcagcgc atcttccatc
     7201 gacaggtaga aacgggaaga accagcatcc ccctgacgac cagaacgacc gcgcaactgg
     7261 ttatcgatac gacgggattc gtgacgctcg gtaccgatga tatgcaggcc acctgcttcc
     7321 agtaccgcat cgtgacgtac ctgccagtcg gctttaattt tttcaatttg ctctgcggtc
     7381 ggattttcca gcgcggcaac ttctgcctgc cagctaccac cgagcacaat atctgtacca
     7441 cgacccgcca tattggtcgc gatagtcacc gcagccggat aacctgcctg agcaacaatc
     7501 gccgcttcgt tggcgtggaa tttggcgttc aggacgttgt gcttaatacc ggctttggtc
     7561 agttcgtttg acaccagctc cgatttttcg atggagatag tacccaccag caccggctgg
     7621 cctttcgcag tacgttcttt gatatcttca atgatcgcct gaattttttc cgcttcagtc
     7681 atgtagacca ggtccggcag atctttacga atcattggac ggttggtcgg aacaacgacg
     7741 gtatccagct tgtagatgga gctaaattcg aaagcttcgg tatcagcagt accggtcatc
     7801 cccgccagtt tttcatacag acggaagtag ttctggaagg tgatcgaagc cagcgtttgg
     7861 ttttcgttct ggatctgcac accttctttc gcttccacag cctggtgcag accatcggac
     7921 cagcgacggc cctgcatggt acgaccggtg tgttcgtcaa cgatgataac ttcaccatct
     7981 ttaacgatgt agtcgacgtc gcgggtaaac agcgcatgag cgcgcagcgc cgccgttacg
     8041 tggtgcatca gcatgatgtt ggccggagag tacagagact ccccttcatc catgatgccc
     8101 tctttcacca gcagttcttc aatcagcacc agaccacgtt cggtcaggtt cacctggcga
     8161 gatttttcgt ccaccgagaa gtggccttcg ccctggaagg tttcggagtc ttctttttcc
     8221 tgacggatca ggtgcggaat aattttattc acgcgtttat acatttccga gctgtcttct
     8281 gccgggccgg aaatgatcag cggtgtacgc gcttcatcga tcaggatgga gtccacttcg
     8341 tccaccagcg catagtgcag tttacgctgt acacgttctt cagggctgaa cgccatattg
     8401 tcgcgcaggt agtcaaagcc gtattcgttg ttcgtaccgt aagtgatgtc agctgcgtaa
     8461 gcttcgcgct ttgccggtgc tggcatgccc ggcaggttga taccgacagt caggccaagg
     8521 aattcaaaca gcggacggtt gttttcggcg tcacgttgcg ccaggtagtc gttgacggta
     8581 actacgtgca cgcctttacc ggttagtgcg ttcaggtaag caggcagcgt tgcggtcagg
     8641 gttttacctt caccggtacg catttcggcg atgcagcgtt cgttaagaac cataccgccg
     8701 agtaactgaa cgtcgaagtg acgcatacca aagacgcgct tacttgcctc acgtaccacg
     8761 gcgaaagctt ccgggatcag attttccagc acttcgcctt tttccagacg tgcacgaaac
     8821 tctgcggttt tccctttcag ttcttcgtcg gagagttttt ccatctccgg ttccatggca
     8881 ttgatgatgt tgaccacttt gcgcatccgg cgcagggtgc gatcgttacg actaccgaaa
     8941 actttagtta acaatttgat tagcataata aaatctcaaa cgccccgcgt tgcggagtta
     9001 ataaaatgaa gtaaaggttt attgttgtta ggtgaggcgt tgagggccag cacggatgcc
     9061 ttgcgcctgg cttatccaga cgggcgtgct gaattttgct tgtggggtaa aatgcgcata
     9121 atcaatgcga taaccctttt cagacggcgt gccttcctgg gtcagcagcg cgctgagcgt
     9181 atccagtaat gcaagatgtt gcgcctgaag aggcaaagat tcttcagcaa cgggcagtgt
     9241 ttgcggtgcc attgcgaaag aaagatgacg gattaccgtg cgaatggcat attgatgcca
     9301 gtaatcaacg gaatagttcg aattcgggcg gcgtgtgttc gcttccagca aggccaattg
     9361 accaaagtta actttggctg aaggctcgtg gttgcgggtt gtcgcttttg cgggcacgtt
     9421 tggttcggcg gcgttgctga gcgcaggcaa acctaaactc gccgcgacca tccctaataa
     9481 gagatgcggc cagaagtagc gtttaccaaa ctgtcgccag cgcgtcagta ttccactcac
     9541 gttattgcca tcccgatcga attcaaacgt atttagcgca caaatcttat cattaaagcc
     9601 tgaagtccac agcatgaatg aagataagtt gtgcgtaaaa gtgcttaaga aaacagcgtt
     9661 cgcaccgttt tttatagctc ttataaaatt caaaaaaaaa cgactggttc acctggccgg
     9721 agagagtgcc agatttgcca gtcgaatttt atacgacagt ataaatgtcg ttatgccagt
     9781 acagctgaag gcgctttgaa ggtcaacggc agttctgcgt cgtcctggaa ggtcacatat
     9841 tcccaagctt cctgtttcgc caggacagcc tgcagcagtt tgttattcag tgcatgaccg
     9901 gatttataag cgataaatgc accaataata ttgtgaccac acatgaacaa gtcaccgatc
     9961 gcatcgagca ttttgtgacg cacaaattcg tcttcaaaac gcaggccgtc ttcgttcagt
    10021 acgcgataat cgtcaacaac gatggcacaa tcgaagctgc cgcccaggca caaaccacgg
    10081 gactgcagat attcgatatc acgcatgaaa ccgaacgtac gcgcacggct gatctggcgc
    10141 ataaacgcat cagcggagaa gttcatcgca tagcgctggt tgcaggaatc aatagccgga
    10201 tggttaaaat cgatggtgaa atccagcgaa aaaccattgt acggcttaaa ttcagcccac
    10261 ttatcgccat cttcgacacg aacagtctct ttgatgcgaa caaatttttt ggcgcagttc
    10321 aactcgtcga taccggcgtc aagcagcagg tatacaaacg gagcggcgct gccgtccatg
    10381 atcgggattt ccggcgcgtt aacttcgata acaatgttat cgatgcccag gcccgcgaga
    10441 gcagcattga ggtgctctac ggttgaaatc cgtacatcat gctcgttgac cagacacgta
    10501 cagagcatgg tatcacgcac agatttggca tcggccggga aatctaccgg tggattcaag
    10561 tcggtgcgac gatagatgac cccggtgttg gccggcgcag ggcgtaacgt cagagtgact
    10621 ttcttgccgg tatgtaaacc gacacccgtc gcctgaacga tacgtttaag tgtcctttgt
    10681 ttgatcatcg tattatctcg ccaaattacc tatccaaccg aagtgtacta tacattcggc
    10741 gggccagttt agcacaaaga gcctcgaaac caaaattcca gtcaattctt aatcagcttg
    10801 cttacgcagg aatgctggga tatccagata atccggctct ttcgcagttt gcggcgcatt
    10861 gtcattcacg actttagcaa ccggcttctg ctcctgggtc agcggagcca tcccatgctg
    10921 ctggtagcga tccatcactg gctgctgaac ctgcttattg gtcaccagag tgatttcagg
    10981 acgtttgtcc atgccgatac ctgtcgcaac aacggttacg cgcagctcgt cattcatatc
    11041 cgggtcaaga gaagtaccga taaccacagt cgcgttgtcg gaagcaaatg cacggatggt
    11101 gttacctacc gtttcgaact catccagacg caggtcgaag cccgccgtga tgttaaccag
    11161 cacgccgcgc gcgccagaca ggtcgatatc ttccagcagc ggagaagaga tagccatttc
    11221 agcagcttct tccgcacggt cttcaccgct cgccacgcca gaacccatca ttgcgtagcc
    11281 catctcagac attacggtgc gtacgtctgc aaagtccacg ttcatcaaac ccggacgagt
    11341 aatcagttca gcgatacctt gcacagcgcc tttcagtaca tcgttcgctg cgccaaacgc
    11401 atccagcagg gagataccgc ggcccagaac tttcagcagt ttgtcgttcg ggatagtgat
    11461 cagagagtcc acatgcttgg acagttcagt gatcccctgc tccgcgaatg ccatacgctt
    11521 cttgccttca aagttgaaag gcttagtgac gacagcaacg gtcaggatac ccaaatcttt
    11581 tgccacttca gcgacgactg gtgctgcacc tgtaccggta ccaccaccca tacccgcagc
    11641 aataaagacc atgtctgcac cttccagcgc cgcacgcaat gcatcgcgat cctcatcagc
    11701 cgcattgcgg ccaacttctg gattagcgcc agcgcccagt cctttggtga taccgctacc
    11761 gatttgaatc gtctgtccaa ccgctgtttt acgcagcgct tgtgcatcgg tatttaccgc
    11821 gaagaattca acaccttcaa tgcgctcgcg caccatgtgt tcaacagcat taccgccgcc
    11881 gccgccgacg ccgatgactt taatcaccgc gtcattggta agttccattg gttcaaacat
    11941 agtttctctc cgatttgtgc ctgtcgcctg aggccgtaat catcgtcggc ctcataaaaa
    12001 ttaaaactct tttcgcagcc aactattgag tcgcttgatc cacgagccaa ctgatgctgt
    12061 aacacgtttt tctacttcag cttcaccgtt aagatgtgac tctttcccat agtgaagcaa
    12121 tcccaccgcc gtcgaataat acggctcctg agcataatcc gttaaaccgg taatgttcag
    12181 cggcgcgccg atacgcactt gcgtatgaaa cacgcgctga gcacaggctg caagaccttc
    12241 gatctgcgct gcgccaccgg ttaatacaat gcctgccgcc aggtgatgtt taaccccttg
    12301 ttggcgaagc ttttcctgca actgcaatat ctcttcgttg accaggttga gcagctcggt
    12361 atagcgcggc tcgatcacct ctgccagggt ctgacgttgc agactccgtg gcggacgacc
    12421 gcctacgctc ggcacttcca cgctctcatc ttttccaacg atggaaccca gcgcacaacc
    12481 gtggcgaact ttaatcgctt cggcgtcgct tggcggcgtg ccaaaggcgt aagcgatatc
    12541 actggtcacg acattgccag cataaggaat taccttagtg tggcgcaatg ccccaccggt
    12601 ataaacggcg atatccattg taccacctcc gatatcgacg acgcagacac ccagttcacg
    12661 ttcatcttcc gtcaataccg aataacttga tgccagtccg gcaaatatca gttggtcaac
    12721 tttcagccca caacgttcaa ccgctttgac gatgtttttc gccatatcgt tgtgacatgt
    12781 gatcaggtgc acttttgcct gcatccgcac gcccgaaagt cctaccggat tcttgatccc
    12841 ttcctgatag tcaatcgcat actcttgcgg gatcacatgc agcacacgat gttcatcgcg
    12901 cacacgcacc gatttcgcgg tatggacgac gttttccaca tcttcttgcg tcacttcttc
    12961 ttcagaaata ggcaccatac caatttcatt ctggcagctg atgtgcttac cagaaagcgc
    13021 cagatatacc gaagagatct gacaatctgc catcaattct gcctggtcaa tggcgcgttg
    13081 tacgcacttg accacggatt cgaggtcgtt caccccgcct ttatccatac cacgtgacgg
    13141 gcagctgccc acgccaatga tattgaccat accgtcgggc agaacttccc ctactaaagc
    13201 ggcaaccttc gcggtaccaa tctccagtcc tactaccagt tttctgtccg tcgccttgat
    13261 cattgttgtt ctgcctgtgc ctgattttgt tgctgagtag attcctctgg cggcaagggc
    13321 gcccagccta ctgccgctcc agagtcataa cgcaaatcaa cgtagctaat ccgtttgcca
    13381 tcggtttgcg ccagctgctg taaaaccgga taaagttcta caaagcgagc caaacgtttc
    13441 atcgtatcgc cccggccaag attgagctta atatcgttat tcagcgtcaa ctgccaggaa
    13501 cgccgcgcgg tcatcgccgt ttccttcaga gtaaatctgt cctttgccag catctgcccc
    13561 atttcgcgat agccctgcaa cacttcattg gcgctgcctt ccgggccata cagcattgga
    13621 agcacctgct tgctggtgcg atctggcggc acgctgaagg tgtttccttc cgcgtctacc
    13681 atatgttgat cattccaccg cgcaatcggc acatattcaa ccagatgaat cttcaattca
    13741 tcaggccact gctttctgac gctcacctgc ttaatccacg gcaggcgttg ttctatttgc
    13801 gtctggatga tgttgacatc ctgggtcata aaggtacccg gctcacccaa tgccaggatc
    13861 gactgccgga tatcgtcatt acgcgtgtaa tggcgttcac cggtcaacac cagctttgag
    13921 agcggcaggc gttgcgcatc ttccatccag cccaacacga cccagccgct caccaacact
    13981 gtcgttaaaa cggtcagcag gaaaaggatc cccgccagac gcgttccatt attgcggcga
    14041 gaagaaacct cttcttcgct gtttcgcgtg ttcagagcag cctgcgacat attagtccgc
    14101 cagttccaga attcgtacta ccaactgcga gaagctcata cctgcctgac gtgccgccat
    14161 cggcaccagg ctgtggctgg tcatacccgg tgaggtattg gcttccagca gataaaactg
    14221 tccatcgctg tccagcataa cgtcaatacg tccccatcct ttgcaaccta acgtcgtcca
    14281 tgctttcagc actaatgcct gcaaattggc ctcttgtgac gcatccagac ctgcggggca
    14341 gaaatactgt gtctcatcag agagatactt cgcctcataa tcatagaagg ttccggacgg
    14401 ttgaatacgt attgacggta aaatttcttc accgagtatc gcaaccgtga actccggccc
    14461 acttagccat ttttcaatca atacttcttc atcgtgctga aatgccaatc ttaatgcatc
    14521 ttgtagagca ttttctgcta ctacttttga cattcccaca ctggaacctt cgcggctcgg
    14581 cttaacgata accggcaaac ccagagcaga aatttctgct aactgcttat cgctcaggcc
    14641 tttttcaaac tctgcgcggg ttaacgctac ccacggcgcg accggtaaac cggcaccttg
    14701 ccacagcagt ttgctgcgta gtttatccat tgaaagcgca gatgccatca ctccgctgcc
    14761 ggtataaggc aagcccatca gctcgagcat gccctgcagc gtaccatctt caccgccgcg
    14821 accgtgtagc gcgataaaca ctttctgaaa acccatcgac ttcagttgcg tcacgtcgac
    14881 ttctttcggg tcgacaggat acgcatcaat accaccttca cgcagcccgg ctaacaccgc
    14941 tgcgccagaa ttcagagaaa cttcccgctc agcggaagtc ccgcccaaca ggaccgcgat
    15001 tttatcagtc atgttgttct tcctccggag tttgcggctt cagtttgatt tcagctaaag
    15061 aacgggcaat ttttccgata ttaccagccc cctgaacgag aatcaggtcg ttaccggtta
    15121 ataccggtgc cagcatctcg gctacctgcg cagggtccgg caccagaatg ggatcaattt
    15181 tcccacgtcc acgaattgtg cgacacagcg aacggctgtc cgctcccgga attggcgctt
    15241 cgccagccgg atacacttcc agcatcaaca gggtatcaac ctgcgtcagc acattggcga
    15301 aatcatcata caagtcgcgc gtacgggtaa aacggtgcgg ctgaaacagc attaccaggt
    15361 ttttatccgg ccagcctgcg cgcgccgctt taatggtggc gtcaacttcc gtcgggtggt
    15421 ggccgtagtc atcgaccagc attgccgtac cgcttttacc attcactggc tccagcggga
    15481 attcaccgag gaaatcaaaa cggcgaccag tccccaggaa gctctccagc gcacgcaaaa
    15541 tagcctcgtc gtcaattccc tcttccgtag caaccgcaac cgcagctgcg gcgttcagcg
    15601 cgttatgacg gcctggcgca ttcagggtga cgcgcatcgg ctctttgtcc tggcgcagca
    15661 gcgtaaagtg cccctgcggg ccaatctgct gataatcttc tacacgcacg tcggcatctt
    15721 cgctgaagcc gtaagtcgtg gtctgacgtc ccacacgcgg taacaattcg cggatcaccg
    15781 gatcatcaac acacatcacc gcacgaccgt aaaacggcag gttgtgcaga aaattaataa
    15841 aagtctgttt taaattctca aagtcgccct ggtaggtatc catgtggtcg gcttcgatat
    15901 tggtgacaat cgccaccatc ggttgcagat gcaggaacga tgcatcactc tcatctgctt
    15961 cggcaatcag gtaccgacca tgccccaaac gcgcatgaac ccccgccgct tttaccagcc
    16021 cgccgttaac gaaggttggg tcgagccccg cttctgcgta gatgctggaa accatcgcgg
    16081 tggttgtcgt tttgccgtgc gttcctgcaa tggcgatgcc atgacgaaaa cgcattaact
    16141 cagccagcat ttcggcacga cggatcaccg gaatacgcgc ttcatgagct gcgacaattt
    16201 ccgggttatc ggcagaaatg gcgctggaaa caacgaccac gctggcatca cgtacgtttt
    16261 ccgggcgatg gttgaaataa atcgtcgctc ccagattcat taactgctgc gtgaccggat
    16321 ttggcgctaa atcggaacca ctgatctgat aaccttcatt ggccagaact tcggcaatac
    16381 cgcccatacc ggcaccaccg atgccgacaa aatgtatgtg ccgaacgcga cgcatttcgg
    16441 gcacgatgga acgcagtttt gccaattgtt gtgtattcat tctttacgcc attaacttct
    16501 taaattcata cgatgcaaaa ggcatcgcta caattacgcc cgggcagccc ggctcacttc
    16561 atttgccact cgctcggtgg catccggaat ggatgcagcg cgggcgcgtt ctgccatggt
    16621 taataaggtt tctcgcgacc acccggccag ggtgttggcg acagcatcca cgctaagctg
    16681 tggctgctcg ataattttgg ctgcgcccgc tttttccagc ggtagcgcat tccagtattg
    16741 ctggcggtct ttatgttgaa acggcacaaa caacgccggt agtcctgccg cggcgatttc
    16801 actcaccgtt aaagcaccgg agcggcaaac gacgacatcc gcccacgcat acgccgccgc
    16861 catatcatca ataaattccg tcactttatg ctgcggttgc cccgcttcgg catacgcctg
    16921 ttcaacggat tgttgcgaac ctttaccgct ctgatgccag atagtgactg aatcacccag
    16981 tttcgcagca acctgcggca ttgtctggtt aagaatgcgt gcgccctggg aaccacccac
    17041 taccagcaca cgaaccggac cttcacgtcc agccaaacgt tgctgcggca acggcagcgc
    17101 cagcacatcg gtacgcaccg ggttacccac cacttccgca ttagggaaag cgcctggaaa
    17161 cgcctgcatc actttggtgg caatcttcgc cagccattta ttggttaagc ccgcaatacc
    17221 gttttgttca tgaagtacaa ccggaatgcc taacgaccac gcggccagac cacctggacc
    17281 tgatacatag ccgcccatac cgagtaccac gtcaggtttg tacgctttca taatcgcccg
    17341 cgcctgacgc caggcgttga agatacgcag cggcgcagct atcagtgctt ttataccttt
    17401 tccacgcaga ccagagatac gaatgaaatc aatttcgatg ccatgttttg gcactaagtc
    17461 cgcttccata cggtcggcag tccccagcca gcgaacttgc caaccctgag ccattagatg
    17521 gtgcgcaacc gccagtcccg ggaatacatg tccaccggtt ccgcctgcca tcaccattaa
    17581 tcgctttcct tgaccactca tcgtgaacct cgtacaaacg cctgcgcttt ctccagacgc
    17641 gtttcataat caatacgcaa cagcatcatg atggctgtcg acataatcag taagctcgaa
    17701 ccaccgtaac tgatcagcgg caatgtcaga cctttggtcg gtaacatccc cgccgccgcg
    17761 cctacgttaa ccagcgcctg gaagctaaac cagatgccaa tagaacaggc gagaaaaccg
    17821 gaaaaacggt ggtcaatttc taacgctttg cggccaatcg acatcgcgcg aaaagcgacg
    17881 aagaatacca ttaaaagtgc cagcaccaca ccgacatacc ccagttcttc gccgataatg
    17941 gcgaaaataa agtcagtgtg cgcttccggc agatactcca gtttttgtac cgagttacct
    18001 aaaccttgtc cccaaagttc gccgcgacca aacgccatca gcgattgcgt taactgatag
    18061 ccgctgccaa agggatcttc ccacgggttc cagaatgcgg taacacggcg gatacggtac
    18121 ggttcggcga gtatcagcaa cacaaccgct gaaatgccca taccgataat ggcaatgaac
    18181 tgccacaatt tcgctcccgc caggaacaac atcgccagcg tagtcacaaa caccaccacc
    18241 accgtaccaa ggtctggctg tgccagcagt aacactgcca acaccagaat cacgcccatc
    18301 ggtttcagga agccgcgcag gttattacgt acttcgtcgc ctttacgcac cagatagttg
    18361 gcgatataac aaaacagcga cagttttgtc agctccgcag gctggatacg cagcaaaccg
    18421 agatcgatcc aacgcgatgc ccctttaacc gagctcccca ctaccagcac gatcatcagc
    18481 aggatgatag atccgagcag catcgtggca ctgtagcgtt gccagaactc catcggcaga
    18541 cgcagcgtaa tgatcgccag aatgaatgcc aggatcagat agacgccatc acgcttcgcg
    18601 aagaagaacg gatcgttggt taagcgttgc cctatgggca ttgacgccga ggtcaccatg
    18661 ataaagccaa tcgccgcaag gccgaaggtc agccacagta aggtgcgatc gtacatgatc
    18721 aggctgtcgg tatctttttc ccgcgagccc atcacccagc cttttagcgc cgtggagatc
    18781 cagaccagga tactgaatcc tggcaggcgc ggcattttca ggcgagggag agataaacgc
    18841 atcaacctaa ctccttcgcc agacgggcaa actcattgcc tcgttgttca aagttcttga
    18901 actgatcaag gctggcacag gccggggaga gcagaaccat atcgcccggc tgaacacgcg
    18961 gagccagcaa gcgcatcgcc tgttccatag tttcggtttg ttctgccact tccgggcgta
    19021 gcgccgccag ctgcgcaccg tcacgaccaa aacagtacaa acgcacgttg tcgccattca
    19081 gatagcgcgc cagtggactg aagtccgccg atttaccatc gccgcccagc aacaaatgca
    19141 gcgtgccgtc tacctgcaag ccattcaacg ccgcttccgt actgccgacg ttagtggctt
    19201 tcgaatcgtt aatccagcgt acgccattgt gctccagcac cacttcaaag cgatgcggca
    19261 gaccggtgaa ggtggttaac gctttcaggc tgctggcgcg cggcaaacca gctgcgtctg
    19321 ccagtgccag tgctgccagc gcattggtgt agttatgctg cccggaaagt ttcatctctt
    19381 tcacgttcag cactttctcg cctttcactc gcagccaggt ttctccctgc tgatgattca
    19441 ggtgatagtc acccatgttg acgccaaagc tgacgcagcg ttcatcagca ccgcgaatcg
    19501 gcattgttaa ggcatcatca gcattgacga cacaaacttt cgcattttcg taaatacgca
    19561 attttgccgc acggtattgc tgcaaaccaa acggatagcg gtccatatga tcttcagtca
    19621 cgttcagaat ggtcgccgcc accgcctgta agctggaagt ggtttccagc tggaagctcg
    19681 acagttccag cacgtacagt tcgcactcag catcaagcag catcaacgct ggcaggccaa
    19741 tattgccacc cacaccaacg ttaacccccg ccgctttcgc catttcaccc actagcgtgg
    19801 tgaccgtgct tttgccgtta gaaccggtaa tcgccacaat cggtgcttgt gcttcgcggc
    19861 agaatagctc gatatcgcca acgatttcga ttccggcatc agcggcagcg cttaaggatg
    19921 gatgcgccag tgcaataccg ggactggcga caatcagatc tgccgccatc agccattcat
    19981 cattcagact gcccgtgtgg cgttctacgg cttcgggtaa tttatccagg ccaggcggtg
    20041 tcatacgcgt atccataacg cgcggcgtca caccgcgagc gaggaaaaag tccacgcagg
    20101 aaagcccggt gaggcccagg ccgataatga cgacattttt accctgataa tcagccatga
    20161 ttaacgtacc ttcagcgttg ccagaccaat cagaaccagc atcagcgaaa taatccagaa
    20221 acgcacaatg acgcgcggtt ccggccagcc tttcagttca tagtggtgat gaatcggtgc
    20281 catgcggaaa atacgttgtc cgcgcagttt aaaggagccg acctgcagga tgacagaaag
    20341 cgtttctacc acgaacacgc cccccataat caccagcagg aattcctgac gtagcagtac
    20401 ggcgataatg cctaacgcac cacctaacgc cagcgaacct acatcgccca taaagacctg
    20461 cgccggatag gtgttaaacc acaggaagcc cagtcctgcc ccgactatcg cggtacagac
    20521 aataaccagt tccccggcgt gtcgcagata cggtatgtgt aaatagctgg caaagttcat
    20581 gttgccggtc gcccacgcca ccagcgcaaa accaccggcg acaaatacgg tcggcataat
    20641 tgccaggccg tcgagaccat cggtcaggtt taccgcgttg ccagtaccca caatgacgaa
    20701 gtaagccagc agaatgtaga acagccccaa ctgcggcatc acatctttaa agaatgggac
    20761 caccagctgc gttgcgggcg tgtctttgcc ggcaaggtac agggcgaagg cgacacccag
    20821 cgcaatgacc gacatccaga aatacttcca acgagcgatc aaccctttgg tgtctttacg
    20881 caccactttg cgataatcat caacaaagcc aataacaccg taacctacca gcaccaccaa
    20941 cacgcaccag acgtacggat tggacgggta agcccacagc agtacggaga tcacaatcgc
    21001 cgtcaggatc ataatcccgc ccatggtcgg cgtaccgcgc ttgctgaagt gtgattcagg
    21061 accgtcgtta cgcaccacct gaccaaagga aagtttttgc aaatgagcaa tcatacgcgg
    21121 gcccatccac aatgagatga acagcgcggt cagcaggctg acgatggcgc gaaacgtcag
    21181 ataggaaaag acgttaaagc cggaataata tttgaccaaa tgttcggcca gccaaactaa
    21241 catgtcccat tctcctgtaa agcgcgtact acctcttcca tggcggcact acgtgaaccc
    21301 ttaactaaaa tcgtaattac ctgttgctca gcaatcagtg atttaagacg cgtaattaac
    21361 gcagttttat cagcaaaatg ttcgccaacg ccgctggcgg tgctgatagc atggctttgt
    21421 ttacccacgc ttaacacgcg gtcaatacca gcagcttttg ccgcctcgcc cacctgtaca
    21481 tggcaggctt cgctttcagc gcccagttcc gccatatcgc ccaccaccag cacgcggtag
    21541 cccggcattt cagccagtac ctgaacagcg gcggtcattg aaccgacatt ggcgttgtag
    21601 gagtcgtcga gcagcaactg gttttctgcc agttggatgg ggaacagacg gcctggaaca
    21661 gctttcagat ttgccagccc cgctttgata gcatcaagcg ttgcgcccac ggacatggag
    21721 agcgcagcgg ctgccagcgc attcgcaata ttgtgacgcc ccggcaacgg cagcagaaca
    21781 tcgacgctac cggttggggt ttgtagggta aattccgtac cgtgcgaggt cacatggata
    21841 ttggtggcgg tgaaatcgct gttggcggca ttgggtgaga aacgccacac tttgcgtgag
    21901 ccaattacgc tctgccagtt cagccagtcg ttgttgtcgg cgttcataat ggcgataccg
    21961 ttttccggca ggccgctaaa gatttcacct ttcgctttcg cgacacccgc aagcgagcca
    22021 aaaccttcca gatgcgccgc tgccaggttg ttgaccagcg cagcttccgg gcgagtcaga
    22081 ctcacagtcc aggctatttc gccctgatgg ttcgcgccaa gttcaataac tgcgtaatcg
    22141 tattccggcg ttaagcgcaa caacgtcatc ggtacaccga tgtcgttgtt gagattgcct
    22201 gccgtataaa gcgtgttgcc gcactggctt aaaatcgccg ccgtcatctc tttaacggag
    22261 gttttgccgg aggaacctgt cagagcaacc acgcgcgccg gaacttgctg gcgaacccat
    22321 gcagccagtt caccaaacgc cagacgcgta tccttgacga ttaactgcgg caggtcgata
    22381 tccagcggac ggctgaccag tagtgcgccc gcgccgccag ctttcgcctg gtcggcaaaa
    22441 tcgtgggcat caaaacgttc gcctttcagg gcaacaaaca ggcagcccgg cgtcagtttt
    22501 cgggtatcag tggttacagc atcaagggtg atatctgcac cttgcagttc accgttgaga
    22561 atgtcggtaa gttggctaag ggttacgcta atcatgcaat cacccccagc agacgcgcca
    22621 ccgtgacgcg atcggagtag tccagacgct gattgccaac aatctggtaa tcttcatggc
    22681 ctttgcccgc gaccagcacc acatcgttct ctttagcctg cataacggcg caagtcaccg
    22741 cttcagcacg gccttccatc actttggcat gtccggcatc taacattccc gccagaatat
    22801 cgttgatgat ggcacgcggt tcttcggtac gcgggttatc gtccgtcacc accgccacgt
    22861 cagcaaactc ttcggcaatt gcgcccatca gtggacgctt acctttatcg cgatcgccac
    22921 cacagccaaa gacacaccac agcttgcccg cacagtgcag acgcgccgcc tgtaaggctt
    22981 tttccagtgc atccggcgta tgcgcgtaat ccaccaccac cgtcggtttg cctggcacag
    23041 tgaacacttc catacgtccg caaaccggtt gcagacgcgc ggcggttttc aacagatcag
    23101 ccaatggata gccgagtgcc aacagtgtcg ccagcgcgag cagcaggttg ctgacgttaa
    23161 aagcgcctat cagatggctt tcaatttcgc catcgcccca acttgagcta aagcgaatcg
    23221 tcacaccgct gtcgtgatag ttcacgtcga tcacttttaa ccagcgtccg tgacagttcg
    23281 gattaatatg atcttccatt gataccgcaa ccgcgtccgg cagctttgcc agccaacggc
    23341 ggcccacttc atcgtcggcg ttaataatcg cctgaccgca atgatgttca gaataaagca
    23401 gccatttcgc agcttcgtag tgttccatat caccatgata atcaaggtga tcgcggctta
    23461 agttggtaaa gaccgacgcc gcaaatttca atgccgccac acggtgctgt accagcccgt
    23521 gggaggaaac ttccattgcg caaaacgtcg cgccctgctc caccagcccc gccagctcat
    23581 gctgaacatc gactgccgaa ccggtggtat tttctgtcgg gatcactttc cccagcagac
    23641 cgttaccaac ggtgcccatt accgcgctgg tttcgccaag cagttggctc cactgcgcca
    23701 gcagctgggt agtcgtggtt ttgccgttgg tgcccgttac gcctacgaga cgtaaattgt
    23761 cagagggttc atggtaaaag cggcccgcca gtgcagataa acgctcgttg agctggctga
    23821 gatagatgac cggtacgccg tgcatttcac ggatttcacc gtcggtcgcc tcatctttcg
    23881 cctctgcaat aatggcagcc acaccttgcg ctatcgcctg cgggatatat cgacgcccgt
    23941 ccgcctgatg acctactaca gctacaaaga gatcgcccgc cgcagccaca cggctgtcga
    24001 gtgtcatctc tcgcagtgct cgcgaaggtg cgtctggcac ccacggagca agaaggtcgc
    24061 gcaaattacg atctgccacc tgtcccctcg ccttgattaa tcacaaattc atttttatcg
    24121 cccgttgtca gcgcatccgg ctcgatgttc atggtacgca atacgccgcc catgatggca
    24181 ccaaagaccg gcgcggaaac ggcgccgccg tagtatttac ccgcctgcgg atcgttgata
    24241 acaacaacca gcgcgaagcg cggctgactc gcaggcgcaa cgcctgcggt ataagcaata
    24301 tatttattga tgtagcgacc gtccggcccg acctttttcg cggtaccggt tttaatggcg
    24361 atacgatagc ctttaatcgc cgccttcacg ccgccgccgc ccggtagcgc cacgctttcc
    24421 atcatatgca ccacggtgcg gacaatggat tccgggaaga cacgttcacc gggaaccggg
    24481 gggtcaactt tggtaatcga cagtgggcga taaatgccgt agctgccgat agttgcgtag
    24541 actcgcgcta actgtaacgg tgttaccatt agcccgtagc cgaaagagaa ggtggccctc
    24601 tctatgtcag accaccgttg tttttgagga tataagccac tgcgttctcc gaccaacccc
    24661 aaattggtcg cttttcccag tccaaaacgt gagtaagtat ctactaacgc tgaggacggc
    24721 atcgctaacg ccagcttgga aacaccgacg ttactcgact tctgtaatac cccggtcagg
    24781 gttaattcgc tgtagcgtgc cacgtctttg atttcgtggc cgttaattcg ataaggaatg
    24841 gtattgagta ccgagttttc ccgcaccacg ccacgttgca acgcggtcat caccaccatc
    24901 ggtttaaccg ttgagcccgg ttcaaacacg tcggtgatgg tacggttacg catcgcctct
    24961 ttcggcgtgc cgctcagatt gttagggttg tatgacgggc tgttagccat cgccagcact
    25021 tcacctgtgt tgacatccac cagcacggcg ctaccagatt cagccttgtt aaaggccacc
    25081 gcgttgttca gttcgcgata aaccagcgcc tgcaggcgtt catcaatact cagcgccagg
    25141 ttgtgcgctg cctggctgtc agtagaagaa atatcttcaa ttacgcgacc atagcggtct
    25201 ttacgcacaa tgcgctcacc cggctgcccg gtaagccatt tatcgaaact cttctcaacg
    25261 ccctcaatcc cttgactatc gacgttagta aagccaatga ggtgagcagt cacttcgccg
    25321 gatggataat aacggcgaga ttcttcacgc agatgaatac ccggcagttt cagttttttg
    25381 atgtagtccg ccatgtcagg gtttacctga cgcgccagat aaataaagcg ccctttcggg
    25441 ttggcgttaa tgcgggcaga aagctgatcc agcggaatat tgagcgcgtt agccagcgcc
    25501 ttccagcggt caccgacgct gataccgcca gcgtcatgca cttctttcgg gtcagcccaa
    25561 atcgctttta ccggcacgct caccgctaac gggcgaccag aacggtcagt aatcatgccg
    25621 cgggaggtgg aaacttgctg aacgcgaaga gaacgcatgt cgccctcttt caccagcata
    25681 tccggggaga taacttgtaa ccacgctacg cgtccgagca gaaaagccag cgccaggaga
    25741 atacagccgc ataacaacgc aaaacgccaa ctaataaagt tggcatgttc ttcctgacgt
    25801 tttggtttct gcgttttcgc cgctgctttc atgcgtcgcg tttatcctta tttttgcact
    25861 acgatatttt cttgtgacgg atcaacatgc tgcatttgca gcttttccgt ggcgatcctt
    25921 tccacccggc tatggtcgcc gagcgcattc tcttcaagga tcaggttgcg ccattcaatg
    25981 tctaaagcat ctcgctccag caccagttgt tcgcgctgag cggtcagtaa acgggtatgg
    26041 tgcgccgtgg ttaccacggt caccgccgtc aaaataatgc aaatgaacag gcagagtggc
    26101 agcttcccaa atcgcaaaag atcgtcaccg ataacaccag gcaatgcatg gcgctcgtgg
    26161 cttcccatcg atcctttaac tttgcttaga gcttctgtca ctctgctgat catgcgttcg
    26221 tcctctctgc gatacgcaga actgaactac gggcacgagg attctcagcc acctcttctt
    26281 cgcccggcat taacttgcct aatgctcgca gctgacggcc acccagtttt ttgagctgct
    26341 cttcagtcat cggtaaccct gccggaactt gcggaccgcg gctgttttca cgcataaaac
    26401 gtttcacaat acggtcttcc agcgagtgga agctgatgat cgaaagccgc ccacccgggg
    26461 ccagcacgtt gagcgagctt tttagcgcct gctctatctc ctccagttca ctgtttaccc
    26521 aaatgcgcac cgcctggaag gtacgggtcg cgggatgttt aaacttatct ttcaccggcg
    26581 ttgcagcagc cacgacttcc gccagttctt tggtgcgggt catcggctgt tcgcggttac
    26641 gctcgacaat ggcgcgggca atgcgtttgg caaaacgctc ttcaccatag gttttcaata
    26701 cccaggcgat atcggcttct tctgcggttt gtagccattc agcggctgac tgcccacggg
    26761 ttgggtccat acgcatgtcc agcggaccat cgcgcataaa ggaaaagcca cgttcagcat
    26821 catcaagttg cggtgaagag acgccaagat cgaggagaat gccgtcgatc ttgccgataa
    26881 gatcgcgctc ggcaacgtat tcgcccagcg cggagaaagg tccgtggatg atggagaagc
    26941 gcggatcatc aatagtcttc gcaacggcga tagcctgcgg gtcgcgatcg atcgccagca
    27001 aacgcccctc ttcgccaagc tgcgagagga tcagacgtga gtgaccaccg cgaccaaaag
    27061 tcccatcaat gtagatgcca tcaggacgga tattgaggcc attaacggct tcatccagca
    27121 gcaccgtagt atgtttatag ttttccatca ttttatagag acaagtcctg cagtcgctcc
    27181 gataagtctc cagtagccaa ctgctctgcg tcgatatctt ccttgacctg ttgatgccaa
    27241 gttgtttcat cccacagctc aaacttgttg aactgtccaa ccagcatcac ttcttttgtc
    27301 agcccggcat gttgccgcag tactggcgcg attaacaatc gacctgcgcc atccatctga
    27361 cattcgctgg catgacctaa cagtaggcgc tgcacacggc gctcaactgg gttcatgctc
    27421 gacagacgcg ataatttttg ctcgataatt tcccattcag gcagggggta aagcagcagg
    27481 cacgggtgat gaatgtcaat ggtgcaaacc atttgaccgg cagcgttctc aagcagctgt
    27541 tcccgataac gggtaggcac tgataagcgc cctttgctgt cgagattgac taacgttgct
    27601 ccccggaaca tgccagcctc accccttatt cccactttgc cccacaattt cccactgaaa
    27661 ggagtttacg gagcggagga aaagcttgtc aagccagtca caaggcatac cgaggtaaaa
    27721 aacccgatgt ttacggctaa tatcagcgtt gtttatttta cgtccaacgc gcgaagcaaa
    27781 ttaacgttat gaatagctgt aagaaaaaat ccctcatcgt catcactgtt ttaaaatgac
    27841 tcactatttt ccacagcaaa tataaagtgt cagtttgcga cgcgagcggc attttaggac
    27901 atatcttccc cggttaacag tccttgttac gtctgtgtgg cgcttgatgc cagcgggttt
    27961 gcgcaaaagc aaagaaaagt gtttgttaaa tcggcaattc atcgccgcat gtaacaaaat
    28021 aatgcaaaaa cgcgctgtcg atatcactta attattgtga atttcagctg gtgagttaat
    28081 taacgggcgg gtttaaaacc atttatcaat aatagtcagg aatagtctta tttactttaa
    28141 gcatattgat gtccagtccc gtactctacg cgccagaggg aaattcacct ggcgcgtatt
    28201 tttgttcgcg gcttagctac ggctgagcac accgcggcga tagagattac gtttaatgcg
    28261 cgttaaacca ggttttggct tacgcggttc gtccaggctt gccaggacaa tctccagcac
    28321 acgctctgcg acatcgcggt gacgttgagc cactgccagc accgggcact gtaagaagtc
    28381 gagcagttcg ttatcgccaa aggtggcaat tgccaggtca gaaggcagtt taccatcgcg
    28441 acgtagcgtg acatccatca ccccttgcaa caacgcaaac gacgttgtga acagcgcctg
    28501 cggcatcaga tgcgtttcca gccatttttc gaataactgg gcagccgcct cccgctcata
    28561 gctgttggca tacaggaaat gcacttcgcg cggatcatct ttccaggcag tacggaaacc
    28621 ttgttcacgc aggaagctga cagaaagctc cggcagcgcg ccaagataaa gcaccgtctc
    28681 ggcgggaaac ttacgtaact cttccgccag catttcggca tcatcctgat cggcaccaac
    28741 cacgctggtg aaatgttcac gatcgagggc gcggtccagc gcgacaatcg ggaacggggc
    28801 gttagcccag cgttgataaa aaggatgctc aggtggcaac gacgtcgaaa caataatggc
    28861 atcaacctga cgctgtaaaa ggtgctcaat gcagcgcatt tcgttgtctg gctgatcttc
    28921 tgagcaggca atcagcagtt gataaccccg ttgccgcgcc tggcgctcaa gatagttagc
    28981 gatgcgggta tagctggtgt tctccagatc ggggatcaca agaccaatag aacgtgtgcg
    29041 tccagcacga agcccagctg ccacggcgtt cgggtggtaa ttgtgctcac gcaccacagc
    29101 catgactttt tcaacggttt tgtcgctcac acggtattgc ttcgctttgc cgttaataac
    29161 atagcttgca gtggtccgcg acactcccgc cagccgagcg atttcatcca gtttcacaat
    29221 tgccccttgc gtaaaatgta aaaaccatag ccattgtact ggtatgggtt aaattcatta
    29281 acatctaagc gcagaaaatt aactgcggca actgctttta ttccaggttc ccactgattt
    29341 cgcaaaaaaa gcccagcgtg acatgttggg ctgcaaattg cacactgaga tcattctcaa
    29401 cgcattattt tatcgccgcg cgaaagtccg accacaccag agcgagcaac ttccacaatt
    29461 ttcgccacct cgcgaatcga tgctaaaaat gcatcaagct tatcgctggt gcctgctaat
    29521 tgaacggtat aaagcgaggg ggtgacatcg ataatttgcc cacggaatat ttccgtatta
    29581 cgtttcactt cgtcacgccc gtaaccgctg gcctgaattt tcaccagcat aatttcccgc
    29641 tcaacatgcg cgccctgccc caactcactc acgcgcaaga catcgaccag cttgtgtaat
    29701 tgcttttcga tctgctcaag tactttttca tcgcccacgg tctggatggt catacgcgat
    29761 aatgtcggat cgtcggttgg cgcaacggtc aggctttcaa tgttgtagcc tcgctgggaa
    29821 aaaaggccaa tcacgcggga taacgcgcct gattcatttt cgagtaagac tgataatatc
    29881 cggcgcataa tcaggttctc tccgttttgc ttaaccacat ttcatccatt ccgcccccgc
    29941 gaatctgcat cgggtagacg tgctcactgc catcgacggt aacatcaaca aacaccaggc
    30001 gattattgcg cacctgttcc agcgcctcgc taagtttgct ttccagctca tgcggatgag
    30061 agatctggat cccgacgtga ccataggctt ccgccagacg gacgaaatcg ggtagtgatt
    30121 gcatataaga ttgtgaatga cggccggaat agatcatgtc ctgccactgt ttcaccatcc
    30181 ccagatagcg gttattgaga ttcaccacca gtacgggcaa ctcgtattgc aacgcggtag
    30241 acagttcctg aatgttcatc tgaatactgc cgtcgccagt gacgcaaacc acggtttctt
    30301 ctggcaacgc cattttgacg cccagtgccg caggtaaacc aaaacccatc gtgccgaggc
    30361 caccggaatt gatccagcga cgcggtttgt cgaatggata ataaagtgca gcaaacatct
    30421 ggtgctgccc gacatcggac gtcacgtaag cgtctccctt cgtcaaccgc caaagagtct
    30481 cgatcaccgc ctgcggttta atcttttcac tgtgagtgtc atatttcagg cactgacgag
    30541 cgcgccactg ttcaatttgc tgccaccagt cgcggatctc atccagtggt tgatgggcgg
    30601 attcttgcga caagagttca agcatttgtt cgaggacctg gcgagcatcc cccacaatcg
    30661 ggatatccgc agtcacggtt ttagaaatgg aagtaggatc aatatcgata tgcagaacag
    30721 tggcatttgg gcagtacttt gccagattgt tcgtcgttcg gtcatcaaat cgtaccccga
    30781 cggcgaaaat cacatccgcg ttatgcatcg tcatattggc ttcgtaggta ccgtgcattc
    30841 ccagcatgcc cagtgcctga cgatgcgttg ccggaaacgc ccccagcccc atcaatgagg
    30901 aaacaacggg cagattcaac gcctccaccg tttctttcaa ctgctgatgg cagcccgcca
    30961 tgattgcccc accgcctacg tagacaaccg gtttttttgc cactaccagc gtttgcagag
    31021 cacgcttaat ttgcccttta tgtccggtag tagtgggatt gtaagaacgc atactgaccg
    31081 actccggcca gacatagggt aatttgttcg ccggattaag aatatctttc ggtaaatcaa
    31141 cgactactgg tccaggacga ccacttgccg ccagccagaa agcctttttc agcacctgcg
    31201 gaatgtcttc cgtttgctta accagaaaac tgtgtttaac cactggtcgc gaaatcccca
    31261 ccatgtcgca ctcctgaaag gcatcgtaac ctatcaacga ggtcgctacc tgcccggaaa
    31321 ggacaactaa tggaatggaa tccatataag cggtggcgat gccggtaatc gcattggtcg
    31381 cccctggacc cgacgttacc agcacgacgc cgacttcccc ggtcgcgcgc gccagaccat
    31441 cggccatatg cactgccgcc tgctcatgac gaactaaaac atgatcgata ccacccacgg
    31501 tatgcaatgc atcgtaaata tcaaggactg cgcctccagg ataaccgaat acttgtttaa
    31561 cgccctgatc gataagcgat cggacgacca tctcggctcc agacaacatc tccatggcct
    31621 gcctcactgt ttgatggaaa aaatgtgtaa aacacattaa ccgtccgatc ttgcttaagc
    31681 aattggcagc cagagacata aacgcagggt aataagaata aacaggagga aagatgaaaa
    31741 gaaacgataa aatcctccat tacgaaggat taaattgata ataaatcggc tgaatcccac
    31801 aacttacagc aatttctcta tttttttaat taatcctaaa atagagagcc cacacattca
    31861 gaataaaatt ctacattcaa taaaaaatgc agaataaacc agacattcat gtctgaccta
    31921 ttctgcattc agttagcgtt tgcaaattga gactaattgt tcttccatcc actgatggcc
    31981 tttatcgcgc ccggcagctt catgccatga gagataacag gttctgctgt tttgttttaa
    32041 cggcagcggt aatacctgta attctaagga ttcagcgaac tcttcagcca gccaacgcgg
    32101 cgcaatagcg accaaatgcg tttgcgacac cacgctaagt acgctcatca ttgccatgcc
    32161 ctgatacgcg atactggctt gcttatctac cgtgtcatac caaggttgac taaatgacgc
    32221 gaaacgatcg agcgaaaccg ccgcatgttg ttcgttataa acatcatgtt tcagtaacgg
    32281 gcctttaatt gttggatgat ttttgctggc taccagcacc atttcatctt taaataatgg
    32341 cacgctggta aattcaggac gatggaagtc ttcataacta atcacaaact ccgtttcctg
    32401 ataacgcagc tgatgttcag tgttctgatt taatgaagac ttgaacataa catgtatatt
    32461 tggcgcaatc tgctcaatgt gattataaat ctgcgaggtc agaatgctgt ctaacgggct
    32521 gcaaacacaa agatgaaata cacgttcact actcgcgggt tcaaaacctg aaccaggcaa
    32581 ttcattttgt actagttgca atgcctggcg aactgaacca aaaagttgaa atgcgcgagc
    32641 agtcggttga ataccacggc cataacgaac aaaaagctcg tcattaaaca tcaccttcag
    32701 gcgtgcaaca gcgttactga ccgcaggttg cgacataccc agaacatgag cggcacgggt
    32761 aatgttttgc tcctgcatca cggcatcgaa aacggttaat aagttgagat cgaccatgcg
    32821 tagctgtggc ttgcttaact ccgccgtctc tgaatgatct gtttgtacct ctggcatact
    32881 taactccact gtcacactta actccctttc ccttattgga atgcagagaa ataatcctga
    32941 agaatatgat ttatcat
//
LOCUS       DAZPEB010000023        32488 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZPEB010000023 DAZPEB010000000
VERSION     DAZPEB010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32488
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.023"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..84
                     /locus_tag="ACHURJ_000977"
     CDS             <1..84
                     /locus_tag="ACHURJ_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1280025.1"
                     /translation="ATLQQDGTIPIEAGEFKATSTFQVTYP"
     gene            197..991
                     /gene="panB"
                     /locus_tag="ACHURJ_000978"
     CDS             197..991
                     /gene="panB"
                     /locus_tag="ACHURJ_000978"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFR1280026.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTASVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1003..1854
                     /gene="panC"
                     /locus_tag="ACHURJ_000979"
     CDS             1003..1854
                     /gene="panC"
                     /locus_tag="ACHURJ_000979"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFR1280027.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKIVELA"
     gene            complement(2146..3042)
                     /gene="rpnC"
                     /locus_tag="ACHURJ_000980"
     CDS             complement(2146..3042)
                     /gene="rpnC"
                     /locus_tag="ACHURJ_000980"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFR1280028.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQGKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFAQRFLSKG
                     MSREDVAEMTNLSLAEIDRLIN"
     gene            3316..3696
                     /gene="panD"
                     /locus_tag="ACHURJ_000981"
     CDS             3316..3696
                     /gene="panD"
                     /locus_tag="ACHURJ_000981"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFR1280029.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3700..4931)
                     /locus_tag="ACHURJ_000982"
                     /pseudo
     CDS             complement(3700..4931)
                     /locus_tag="ACHURJ_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
     gene            complement(4995..5435)
                     /locus_tag="ACHURJ_000983"
     CDS             complement(4995..5435)
                     /locus_tag="ACHURJ_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFR1280030.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5540..6310)
                     /locus_tag="ACHURJ_000984"
     CDS             complement(5540..6310)
                     /locus_tag="ACHURJ_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1280031.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLATTFGVLVVFIVVFYLICWSLIQRGRGLRS"
     gene            complement(6307..7233)
                     /locus_tag="ACHURJ_000985"
     CDS             complement(6307..7233)
                     /locus_tag="ACHURJ_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1280032.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKLETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7342..8004
                     /gene="can"
                     /locus_tag="ACHURJ_000986"
     CDS             7342..8004
                     /gene="can"
                     /locus_tag="ACHURJ_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFR1280033.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPHFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8045..8581)
                     /gene="hpt"
                     /locus_tag="ACHURJ_000987"
     CDS             complement(8045..8581)
                     /gene="hpt"
                     /locus_tag="ACHURJ_000987"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFR1280034.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8787..11177
                     /gene="gcd"
                     /locus_tag="ACHURJ_000988"
     CDS             8787..11177
                     /gene="gcd"
                     /locus_tag="ACHURJ_000988"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFR1280035.1"
                     /translation="MAINNTGSRRLLVTLTVLFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPTEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPKLKTNESFQHVTCRGISYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11224..12774)
                     /gene="cueO"
                     /locus_tag="ACHURJ_000989"
     CDS             complement(11224..12774)
                     /gene="cueO"
                     /locus_tag="ACHURJ_000989"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFR1280036.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12940..13287
                     /locus_tag="ACHURJ_000990"
     CDS             12940..13287
                     /locus_tag="ACHURJ_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFR1280037.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13392..14258
                     /gene="speE"
                     /locus_tag="ACHURJ_000991"
     CDS             13392..14258
                     /gene="speE"
                     /locus_tag="ACHURJ_000991"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFR1280038.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPSGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14274..15068
                     /gene="speD"
                     /locus_tag="ACHURJ_000992"
     CDS             14274..15068
                     /gene="speD"
                     /locus_tag="ACHURJ_000992"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFR1280039.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15105..15467)
                     /gene="yacL"
                     /locus_tag="ACHURJ_000993"
     CDS             complement(15105..15467)
                     /gene="yacL"
                     /locus_tag="ACHURJ_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFR1280040.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15642..18239)
                     /gene="acnB"
                     /locus_tag="ACHURJ_000994"
     CDS             complement(15642..18239)
                     /gene="acnB"
                     /locus_tag="ACHURJ_000994"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFR1280041.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18594..20447
                     /locus_tag="ACHURJ_000995"
     CDS             18594..20447
                     /locus_tag="ACHURJ_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFR1280042.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAAVETQSTQ
                     LAVSDAAAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDALLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSNEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTASPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FIDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVRERMQTNPQIQQRREAARERIQPASPEQRQ
                     AVREKMQTNPQIQQRRDAARERIQSASPEQRQVFKEKVQQRPLNQQQRDNARQRVQSA
                     SPEQRQVFRERVQESRPQRLNDSNRTARLNNDQRSAVRERLSERGARRLER"
     gene            complement(20518..21942)
                     /gene="lpdA"
                     /locus_tag="ACHURJ_000996"
     CDS             complement(20518..21942)
                     /gene="lpdA"
                     /locus_tag="ACHURJ_000996"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFR1280043.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22267..24159)
                     /gene="aceF"
                     /locus_tag="ACHURJ_000997"
     CDS             complement(22267..24159)
                     /gene="aceF"
                     /locus_tag="ACHURJ_000997"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFR1280044.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAQAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(24174..26837)
                     /gene="aceE"
                     /locus_tag="ACHURJ_000998"
     CDS             complement(24174..26837)
                     /gene="aceE"
                     /locus_tag="ACHURJ_000998"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFR1280045.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHSI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(26998..27762)
                     /gene="pdhR"
                     /locus_tag="ACHURJ_000999"
     CDS             complement(26998..27762)
                     /gene="pdhR"
                     /locus_tag="ACHURJ_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFR1280046.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28303..29676
                     /gene="aroP"
                     /locus_tag="ACHURJ_001000"
     CDS             28303..29676
                     /gene="aroP"
                     /locus_tag="ACHURJ_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFR1280047.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNSGPQATVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29719..30573)
                     /gene="ampE"
                     /locus_tag="ACHURJ_001001"
     CDS             complement(29719..30573)
                     /gene="ampE"
                     /locus_tag="ACHURJ_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFR1280048.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARVTMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGIWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30570..31121)
                     /gene="ampD"
                     /locus_tag="ACHURJ_001002"
     CDS             complement(30570..31121)
                     /gene="ampD"
                     /locus_tag="ACHURJ_001002"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFR1280049.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31209..32102
                     /gene="nadC"
                     /locus_tag="ACHURJ_001003"
     CDS             31209..32102
                     /gene="nadC"
                     /locus_tag="ACHURJ_001003"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFR1280050.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32305..>32488
                     /locus_tag="ACHURJ_001004"
     CDS             32305..>32488
                     /locus_tag="ACHURJ_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFR1280051.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHG"
BASE COUNT         7833 a         8834 c         8003 g         7818 t
ORIGIN      
        1 gccacgctac aacaggatgg aactatacca atagaagctg gtgagtttaa agccaccagt
       61 actttccagg taacctaccc ttaataagtc caccgcaccg ccatcatctg gcggtgcggt
      121 aattgataaa tcattgcccc caccaatgtg actgataaaa tgccaggcaa cacgacatca
      181 tttatcagga cacgttatga aaccgaccac cattgcttca cttcagaaat gtaagcagga
      241 taaaaagcgt ttcgcgacca tcaccgctta cgactacagc ttcgcgaaac tatttgctga
      301 tgaaggactt aacgtcatgc tggtaggcga ctcgctgggc atgacggttc agggtcacga
      361 ctccaccctg ccggtgaccg ttgccgatat cgcctaccac actgcctccg tacgtcgtgg
      421 tgcgccaaac tgcctgctgt tggctgacct gccgtttatg gcgtatgcca cgccggaaca
      481 agcctttgaa aacgccgcaa cggttatgcg tgccggtgcc aatatggtca aaattgaggg
      541 cggtgagtgg ctggtcgaaa ccgtaaaaat gctgaccgaa cgtgccgttc ctgtatgtgg
      601 tcacttaggt ttaaccccac agtcagtgaa tattttcggt ggctacaaag ttcaggggcg
      661 cggcgatgaa gcgggcgatc aactgctcag cgatgcatta gccttagaag ccgctggggc
      721 acagctgctg gtgctggaat gcgtgccggt tgaactggca aaacgtatta ccgaagcact
      781 ggcgatcccg gttattggca ttggcgcagg caacgtcact gacgggcaga tcctcgtgat
      841 gcacgacgcc ttcggcatta ccggcggtca cattcctaaa ttcgctaaaa atttcctcgc
      901 cgaaacgggc gacatccgcg cggctgtgcg gcagtatatg gctgaagtgg agtccggcgt
      961 ttatccgggc gaagaacaca gtttccatta aggagtcacg ttgtgttaat tatcgaaacc
     1021 ctgccgctgc tgcgtcagca aattcgccgc ctgcgtatgg aaggcaagcg cgtggcgctg
     1081 gtgcctacta tgggtaatct gcacgatggc catatgaagc tggtcgacga agccaaagcc
     1141 cgcgccgatg tggtcgtcgt cagtattttc gttaacccga tgcagttcga ccgcccggaa
     1201 gatctggcac gttatccacg gaccttgcag gaggactgcg agaagctaaa caaacgtaaa
     1261 gtggatttag ttttcgcccc ttcggtaaaa gagatctacc cgaacggtac tgaaacccat
     1321 acttacgttg acgttcccgg gctttcgacc atgctggaag gcgcgagccg tccggggcat
     1381 tttcgcggcg tttcgactat cgtcagcaag ctgtttaacc tggtccagcc ggacatcgcc
     1441 tgcttcggtg aaaaggactt tcagcaactg gcgctaatcc gcaaaatggt tgccgatatg
     1501 ggcttcgata ttgaaattgt cggcgtcccg attatgcgcg ccaaagatgg ccttgcgctc
     1561 agttcccgta atggttatct gacggcggaa caacgcaaaa ttgcgcccgg tctgtacaaa
     1621 gttttaagtt caattgccga caaattgcag gctggcgagc gggatcttga tgaaattatt
     1681 gccattgcag ggcaagaact gaatgaaaaa ggcttccgcg ccgatgatat tcagattcgc
     1741 gatgccgaca cgttgctgga agtctctgaa accagcaaac gagctgtaat tctggtagcc
     1801 gcctggcttg gcgatgcccg cctgatcgac aacaaaatcg tcgaactggc gtaatgcttt
     1861 tactctaaat aattcaagtt gcaggaaggc ggcaagcgag tgacaaattc gtcaggaacg
     1921 aatttgccca gccgcaggct ggccttcggt gagagacatg gatgtctctc attaatcccc
     1981 aggagcttac ataagtaagt gactggggtt agcgaacgca gacacagcac atgcaattta
     2041 aagtatgacg agtataactg gcgctacggc tgatggcgcc aattattaat ttgccccacg
     2101 tcatggcgat ggcgtctgta agcaattatt cataacgcag acaacttagt taatcagcct
     2161 atcaatctca gcaagagata aatttgtcat ctctgcaacg tcttcccgag acatcccttt
     2221 actcagaaaa cgctgggcga attcttgcct ttcctcctgt cttccctgct gaatcccctt
     2281 ctcaatccct ttctcttcaa accactgcgc cagcgtcatc atagactccc ctcccgtttc
     2341 cctgtctctc agcacaccat aaaacaaatc cgcttgttca gtatgaccgc gttgcagcat
     2401 atagttttgc atggcaacta actgacttcc gctagtgtac ccttcgtcaa tcagcgtgac
     2461 cagttgctcc agcaataaca ttaagtcgcg ctggcgaata tgtttttgca gtagttcgag
     2521 aatcgcaatc cgccgatgtt gcatgatttc gtcatccggt gtgatggtga tatccaccag
     2581 cgggaaagga ctgttataga cgcgtcgtgc cagctccggc gagtaaaata tatcaaacca
     2641 gcacattgag agcggataag gtgtggcctc gccctgataa aacagtatcg gcaccaccag
     2701 cggcagctta tcgtgatccg cctccagatg ccggtgcatg gcagcaatag aataacgcat
     2761 catgcgaaag gccattttct tatccggctt gccttggtgt tcaatcacaa catgcagata
     2821 accgggattg ccctgcattt gcacggaata gagcacgtcc gtgctgtgtc ctttcaggct
     2881 ctcttcaatg aaactccccg actctaaatg aagcgtgttg aggtcacaaa gttctcgtaa
     2941 ttccactggc aaatgtatct ccagaaagtc gcgagccgtc tccgcgtgca ttaaaaattg
     3001 tttaaacact gcatcatgcg gtgtggtact cggtgcatcc atcttccctg ccttccgtgt
     3061 tgtcttgcgt gaaggcagta tccagtcaag gcgtgccatc gtcagccacc tctttagtgt
     3121 ttaccgagca gcgttcagag aaatttcaat tggttacagc cgttacatgg cacgcttcgg
     3181 cacggtacgc aaactgctga tgtaaatgcc gtcagatgag tagacactaa acaaaaatcg
     3241 ggcaatactg cgtgagaatt tcctcaaagc aggcatcgcc tgcttcgtta acgacagggt
     3301 agaaaggtag aagttatgat tcgcacgatg ctgcagggca aactccaccg cgtgaaagtg
     3361 actcatgcgg acctgcacta tgaaggttct tgcgccattg accaggattt tcttgacgca
     3421 gccggtattc tcgaaaacga agccattgat atctggaatg tcaccaacgg caagcgtttc
     3481 tccacttatg ccatcgcggc agaacgcggt tcgagaatta tttctgttaa cggtgcggcg
     3541 gcccactgcg ccagtgtcgg cgatattgtc atcatcgcca gcttcgttac catgccagat
     3601 gaagaagctc gcacctggcg acccaacgtc gcctattttg aaggcgacaa tgaaatgaaa
     3661 cgtaccgcga aagcgattcc ggtacaggtt gcttgattgt tatccctgcg gctggttact
     3721 caccagccgc gacatcgtct ccagcgaatc cgttcttaag atataaagtc gttttagtaa
     3781 caacggatta tcccccggtt ttactttgcc tttcatggtt gtcaccgcca ggtgaaatcc
     3841 ggcatcgttt gctgccttca cggctttgtc attaaatccg ccaaacggat acgaaagata
     3901 ccagacatgc ggattaaatt gcgccagagc gcggcgtgaa cgtgcaaaat caaacagaat
     3961 attgtgttca ctacggctcg gtaatatggg gcggcgataa ccatccaccc gatgcaaaaa
     4021 atgggtatgt gactggaaat caaaaacatc gcgaatttcg ttaagttcag aaacgctcat
     4081 aaattgcagc gattttgggt cccacttctg cgggtgacgt ttgatgcgcg aggtaacaat
     4141 aaacgccgtc gccttcatgc catattgttt caacacggga tacgcatagc ggctcaccga
     4201 tttgaggcca tcatcaaagg taatcaccac cgctcgcgca gggagattga tcttattctt
     4261 cacgtaacct tccagctgca ccatgctcag tgtcgcgtat cccctgtcgc gcagccaggc
     4321 catctggtta ttgaaagcgc gtactgaggt ggtcgtcgaa gtatggcgaa aacgggtgtt
     4381 ttcttcatcg cgcagaatat ggtgataggt tagcaccggc aggccattat cgggttgggc
     4441 atccagcgcg ctgatatagg ccagtcgatc gccaatacgg atctgatacc aggtttgatt
     4501 taacctgtct ttcagtttat gcaaaatcgg gtagcgcaaa ttgtccgcca gtaccccata
     4561 atggcgcact gcccgcactc ggcgcgttat agaccggcgt atctttccag gtaattaagt
     4621 tctgattact cagcggcttg ttgaggtcac ccaaaccgtc ttcaactttt tgtcgcccct
     4681 gaactggctc gagatgccct ttatcgataa accctttacc aaagccaaaa ttaaatgcgt
     4741 aataacttgc ggcagtgggc tccacggcaa tgatttgccc ggcccgaata ttccccacgg
     4801 tcaccatctt gtcgccaatt tgcgcccaga tcgcagcatt ttcgatggtt tgcatataac
     4861 gggcaggtaa cgcggcacta acgcttgcgg taaacagcat cagcaggatg aataacagct
     4921 tgtttataca tgaaaaaata accgagggtg aaacaacgct tgtattgtag caaaagcgcc
     4981 atcagaatcg cgggttaata ctcatacaaa ttatgtttga ggacaaaagg cggatttttt
     5041 tgttgttggt gccaaagact actcacctcc ggcgcaccca gttcgacaat acgctcgcga
     5101 aactctgaac tggtcatggt ttcacggcaa gccatcatct gttcaattaa cggtaacgtg
     5161 acaccagaaa tcacttcaca acgggagtgt ttatggctta ataatgaagc cacgcggtac
     5221 ggtggcgctc cggctatatc ggttaagaag atgacaccct caccagagtc cgcttcatgc
     5281 agagcatcgc acatcatgcg gctgagcata ttagagctta atccgcgcca gaaattcacg
     5341 gcccggcact gaagaagtgc cccatgtttt ttctccagcg catccagtat ctcttgcgcc
     5401 cggtcatcgt gacaggtaat tacccaacct aacatcgcct tcctccttag ccgaaaatca
     5461 gtctatcctg accattgttg ataaagggtg atagccgtca aaactcctct cccccgaatc
     5521 cagaggagag gaaatagcct tagctacgca aaccacgtcc acgttggatc agcgaccaac
     5581 agatcaaata aaacaccaca ataaagacca ccagtacgcc aaaggtagtg gccagcggca
     5641 catcatttat accgaggaag ccgtagcgga aaccactgat catataaacg attgggttca
     5701 ggtgcgacag cccttgccag aacggcggca acaaagtcag ggagtaaaac accccgccca
     5761 aatacgtgag tggcgttaac acaaaggttg gcaccaggct gatgtcatcg aacgttttgg
     5821 caaacacgcc gttcagcaaa cccgcaaggg agaatagcac cgccgtgagc accagggtta
     5881 aggcaacgaa tacccacgaa tgcacctgaa acggcacaaa aaacagtgaa attgccgtca
     5941 ccagaatgcc aacaaacaga ccacgcgcca cgccaccgcc gacatatccg gcaataatga
     6001 cgtgagtcgg aaccggcgct accagcagct cttcaatatt acgctggaac ttggcaccaa
     6061 aaaatgatga cgcgacgtta gcgtaggcat tggtgatcac cgacatcatg atcagccccg
     6121 gtacgatgaa ctgcatatag ctgaagccat gcatatcgcc aatacgcgaa cctatcaggt
     6181 taccgaagat aataaagtaa agggtcatgg tgatgactgg cggcaccagc gtctgcaccc
     6241 agatacgcat aaagcgatgg atctctttcg cccagatgct ttttagcgcc acccagtaaa
     6301 gatgcatcat gcgcgatctc cttgtttttc attaaccagt gaaacaaaca gctcttccag
     6361 acggttagct ttgttacgca tacttaatac ctgaatgccc tgctcactta actgcgtaaa
     6421 tacgctgttg atcccctgct cacgcagcac ttcaacttcc agcgtcgcgg tatcgaccag
     6481 tcgatactga tagccatcga gcttcggtaa cgggcttttc ggtgcgagat cgagaataaa
     6541 ggtttccaat ttcagcttcg ccagcagcgc cttcatcgag gtattttcca ccagctcacc
     6601 gtgttgaata atgccgatat tgcggcacag catttctgct tcttccaggt agtgtgtagt
     6661 gagaatgatg gtggtgcctt tgtcgtttaa atccttcaaa aagccccaca ttgagcggcg
     6721 aagttcaata tccacgcctg cggtcggttc gtcgagaatc agtagtttag gttcatgcat
     6781 taacgcacgg gcaatcatta aacggcgctt catcccgcca gataacatac gcgcacgttc
     6841 gttgcgtttt ccccataaat cgagttgttt gagatatttt tcgctgcgga tgtacgcttc
     6901 tttgcgctcc acgccgtagt accctgcctg attcaccaca atttgctgca cggtttcaaa
     6961 cgggttgaag ttaaattcct gcggcaccag tcccaactga cgtttagcgt tcacgacatc
     7021 cttctcgaga tcgtaaccaa atacgctgac ccgcccggag gttttattta ccagagagct
     7081 gataataccg atagtggtcg atttcccggc cccgttcggc ccgagaagcg cataaaaatc
     7141 acccgcttcg acctgcaaat ctattccacg aagcgcctga acgccgcctg gataggtttt
     7201 tttaagctgt tgaagttcca gtgcaatggt cataaatttt tacttacctt acgttcttac
     7261 actttatctg tggtttaaat cgtcccggag ttaccctata ttagccaaac gtaattattt
     7321 ggttacaggt cgttaacctc catgaaagac atagatacac tcatcagcaa caatgcacta
     7381 tggtcaaaaa tgctggtgga agaggatccc gggttttttg agaaactggc acaagcgcaa
     7441 aaaccgcact ttctatggat tggatgttcc gacagtcgcg ttcctgcaga acgtttaacc
     7501 ggtcttgagc cgggcgaact ctttgttcac cgtaatgttg ctaacctggt cattcacacc
     7561 gacctgaact gcctttccgt ggttcagtat gcagtggatg tactagaagt tgaacacatt
     7621 attatctgtg gccactacgg ttgcggcggc gtacaagccg cagttgaaaa cccggaactg
     7681 gggcttatca acaactggct gctgcatatc cgcgatatct ggttcaaaca tagctcattg
     7741 ctcggcgaaa tgccgcaaga gcgccgtctg gataccttgt gtgaactgaa cgtcatggaa
     7801 caggtgtata acctaggcca ctccaccatt atgcaatcag cgtggaaacg cgggcagaaa
     7861 gttaccattc acggctgggc ctatggcatt cacgacggct tgctgcgtga tctggatgtt
     7921 actgccacca accgcgaaac ccttgagcaa cgttaccgtc acgggatttc caacctcaag
     7981 ctgaaacacg ccaaccacaa ataaaaatgc catgccggat gcaacacatc cggcaacttc
     8041 acacttactc gtccagcaga atcactttgc cgatatacgg cagatgacgg taacgctgtg
     8101 cgtaatcaat gccgtaaccc accacaaact catccgggat cgagaaaccg ataaattcta
     8161 ccgggacgtt cacttcacga cgggacggtt tatccagcag cgtacaaatc gccagcgact
     8221 tcggttcgcg caggcttaag atctcacgca ctttcgacag tgtattcccc gagtcgatga
     8281 tatcttcaac aatcagcacg tccttgccac ggatatcttc atccagatct ttgaggattt
     8341 tcacatcacg ggtggtggac atgccgctac cgtagctgga ggcggtcata aagtcgactt
     8401 catgagatac ctgaacttca cggcacaggt ccgccataaa cataaatgag ccacgcagca
     8461 gacccaccag caccatatcg ctgccgctgt ctttgtaacg ctcagtaatc tgacgaccca
     8521 gttcggcgat acgcgcttta atctccgctt cggggatcat tacttctaca gtatgtttca
     8581 tatctctaac catatgattt aaaaacaaat cacttcgcac gggcaggttt tggcaccgca
     8641 acaaagcgca agacacgcag tataccagta aaactattca ttcgcggaat ctgttaataa
     8701 agctcgaatt gtgatgacga tcacacatgt taaaacctat aattaattgc tactaaaata
     8761 ttaatgaatt gaaatggtgt ctctttatgg caattaacaa tacaggctcg cgacgattac
     8821 tcgtcacgct aacagtcctt tttgcagcgc tttgcgggct gtatctactc attggcggag
     8881 gctggctggt cgcgattggc ggctcctggt actaccctat cgctggcctt gtgatgctcg
     8941 gcgtcgcctg gatgctgtgg cgcagtaaac gtgccgcgct ttggctatac gccgccctgc
     9001 tgctcggcac catgatttgg ggcgtctggg aagttggttt cgacttctgg gcgctgactc
     9061 cgcgcagcga tattctggtc ttcttcggca tctggctgat cctgccgttt gtctggcgtc
     9121 gcctggtcat tcctgccagc ggcgcagttg ccgcactggt ggtggcactg ctgattagcg
     9181 gtagtatcct gacctgggcc ggatttaacg atccgcagga aatcaacggc accttaagcg
     9241 ccgatgccac acctacggaa gctatctcac ccgttgccga tcaggactgg cctgcctatg
     9301 gtcgcaatca ggaaggtcaa cgcttttcac ctctgaaaca aattaacgcc gataacgtcc
     9361 ataatctgaa agaagcctgg gtgttccgta ctggcgatgt gaagcagccg aacgatccgg
     9421 gcgaaatcac caatgaagtg acgccgatta aagtgggcga caccctttac ctgtgtaccg
     9481 ctcaccagcg cctgtttgcg ctcgatgccg ccagcggcaa agagaaatgg cattacgatc
     9541 ctaagctgaa aactaacgag tctttccagc acgtaacctg ccgtggcatc tcttatcatg
     9601 aagccaaagc agaaacggct tcgccggaag tgatggcgga ttgcccgcgt cgtatcattc
     9661 ttccggtcaa tgatggtcga ctgattgcga ttaacgctga aaacggcaaa ctgtgcgaaa
     9721 ccttcgccaa taaaggcgtg ctcaatctgc aaagcaatat gccagacacc aaaccgggtc
     9781 tgtatgaacc gacttcgcca ccgattatca ccgataaaac catcgtgatg gcaggttcag
     9841 ttaccgataa cttctcaacc cgcgaaacgt ctggcgtgat ccgtggtttt gatgtcaaca
     9901 ccggggagct gctgtgggct tttgatcccg gcgcgaaaga tccgaacgca atcccgtctg
     9961 acgaacacac ctttaccttt aactcgccaa actcctgggc accagcggcc tatgacgcga
    10021 agctggatct ggtctatctg ccgatgggcg tgaccacgcc ggatatctgg ggcggtaacc
    10081 gcacaccgga acaggaacgt tatgccagct cgattctggc gctgaatgcc actaccggga
    10141 aactggcgtg gagctaccag accgttcacc acgacctgtg ggacatggat cttccggcac
    10201 agccgacgct ggcggacatc accgttaatg gtcagaaagt gccagttatt tacgctccgg
    10261 cgaaaaccgg caacattttt gtgctcgatc gtcgcaatgg cgaactggtg gttccggccc
    10321 cggaaaaacc ggtgccgcaa ggtgctgcga aaggcgatta cgttactccg acccagccgt
    10381 tctctgaact gagcttccgt ccgaagaaag atttgagcgg tgcggatatg tggggagcca
    10441 ccatgtttga ccaactggtg tgccgcgtga tgttccacca gatgcgctat gaaggcattt
    10501 tcacgccgcc atctgaacag ggcacgctgg tcttcccggg taacctggga atgtttgaat
    10561 ggggcgggat ttccgttgat ccaaatcgtg aagtggcaat tgccaaccca atggcactgc
    10621 cgtttgtttc gaaactgatc ccacgcggtc cgggtaaccc gatggagcag ccgaaagatg
    10681 ccaaaggcac gggtacggaa tccggcattc agccacagta cggtgtaccg tatggtgtca
    10741 cgctcaaccc gttcctctca ccgtttggtc tgccgtgtaa acagccagca tggggttata
    10801 tctcggcgct ggatctgaaa accaatgaag tggtgtggaa gaaacgtatt ggtacgccgc
    10861 aggacagtat gccgttcccg atgcctgtac cggtgccgtt caatatgggt atgccgatgc
    10921 tgggcgggcc aatctccacg gcgggtaacg tgctgtttat cgccgctacg gcagataact
    10981 acctgcgcgc ttacaacatg agcaacggtg aaaaactgtg gcagggtcgt ttaccagcgg
    11041 gtggtcaggc gacgccaatg acctatgaag tgaatggtaa gcagtatgtg gtgatctccg
    11101 caggcggtca cggttcattt ggtacgaaga tgggcgacta tattgtggct tatgccctgc
    11161 cggatgatgt gaaataacca taatgcccgg agagatccgg gcatatttcc gaatacggtc
    11221 tttttatacc gtaaacccta acatcatccc cgtatcttca tgctccagca gatggcagtg
    11281 cgccatataa gcatgttctt tcggtgcgtc gtgattaaac ttcaccagca cttcactgac
    11341 attgccttct accttaacgg tatctttcca gcccgcgcga tgagccgctg gcggtttgcc
    11401 attttctgac aagatacgga actgcgtgcc gtgaatatgg aacggatgca gcatcatgtc
    11461 gcccacgcca gagataaccc aacgttcgta ctgccctttc gccgccgcaa acatcggctt
    11521 attcatatca aacgcctgac cgttgatttt attggcatgg tggaaatcga acttcccgcc
    11581 gtggttcata tgattcatat taccgtgccc catatggccc atcatctggc tgtgatccat
    11641 tccggccatc gcctgatcgc catatttctc cattagcatc tgcataccca tcatatcgag
    11701 catcgggtcc atagaaagtt gcagcttgcg taccgtcagc ccttccagcg aaggtaacgc
    11761 aggcaggcta cttaatgtgt ctggcaaagc accggaggca ctaatagcaa tcggctgaat
    11821 ccgcattacc ggatgcggct tgtcaaacgg cgcaatcgcc atccccatct ggctgaccgg
    11881 tagcgtcacc aggtcaaagg gtttgttgtc gttaacctcc accagcactt caaaacgctc
    11941 gcccatcagc accggcagct cgctcacctt caccggttca ggtagcagac caccgtcgct
    12001 ggcaatcaca tacagcgggc gattgtcgct ggtggcgaaa ttgagcgagc gggcattaca
    12061 gccattgagc aaacgcaggc gcagccaacc acgcggggca gcgtgttgtg ggtagattgc
    12121 accgttggtc agcagcgtat cgccaaacca gcccacggcg gcggtcatca catccagttg
    12181 ataatcaatc tgcccatcag cgttaaattt tttatcctga acaatcaccg gaacatcatc
    12241 gataccccac tgttttggca gcattaattt caggatctcg tcatcttcaa ttaccaccag
    12301 cccagccaac cccatcgcca cctgtcgccc agtcttgcca tgttgatgtg gatggaacca
    12361 gcaagtagcg gcaggttgat caacgttcaa cgtcaccgag cgcttgccac ctggcggaat
    12421 aattccctgc gggccgccgt cgacttcacc cggtacttcc agcccgtgcc agtgcaacgt
    12481 cgtctcttcc gtcagttggt tatagatatc aaccgttacc gctttgccgc gctgtaattt
    12541 caccgccggc cccagcagat tgccgttata gccccaggta gttgcagttt tctcgccaaa
    12601 ggtagactga cctgcgccaa tagttaactg aatgcgatta cgggcatcgg tcgtgagcaa
    12661 atcagggatc ggtaacgttg ggcgttccgc cgcaaagact gcgcggctcc acagcggcaa
    12721 ggctgaagcc acacccagcg cgacggaata ttttaagaaa tcacgacgtt gcatagttat
    12781 ttccttattc ttaagcaggc gaaacaaaat caaacgttga gcatagtcct tccccttacg
    12841 ggaaggtcaa gccgcagaca caatttaatc gacaataatg gtcgtcgcct cgctcgcagt
    12901 gtgctaacgt ttatcttctt taagcccctg gtagaagcaa tgaagacgtt tttcagaaca
    12961 gtgttattcg gcagcctgat ggccgtttgc gcaaacagtt acgcgctcag cgagtctgaa
    13021 gccgaagata tggccgattt aacggcagtt tttgtctttc tgaagaacga ttgtggttac
    13081 cagaacttac ctaacgggca aattcgtcgc gcactggtct ttttcgccca gcaaaaccag
    13141 tgggatctca gtaattacga caccttcgac atgaaagccc tcggtgaaga cagctaccgc
    13201 gatctcagcg gcattggcat tcccgtcgct aaaaagtgca aagccttggc ccgcgattcc
    13261 ttaagcctgc ttgcctacgt caaataatcc ctgatacctt tttgtagaaa tattgaccgt
    13321 gcatccgcgg tcaatgttag ctattatgtt gcgccctttt ttacgggtgt taacaaagga
    13381 ggtatcaacc catggccgaa aaaaaacagt ggcatgaaac gctacacgac cagtttgggc
    13441 agtactttgc ggtagataac gttctgtatc atgaaaagac cgatcaccaa gatctgatca
    13501 tttttgagaa cgctgcattt ggtcgcgtaa tggcgctgga tggcgtagta caaaccaccg
    13561 agcgcgacga gtttatctat catgagatga tgacccatgt tccgctactg gcccacggtc
    13621 acgcgaaaca tgtgctgatt atcggcggcg gcgacggtgc catgctgcgt gaagtaaccc
    13681 gacataaaaa cgttgagtca atcacgatgg tagaaatcga tgcgggtgtc gtgtcgttct
    13741 gccgtcagta tctgcccaac cataacgccg gtagctacga cgatccgcgc tttaagctgg
    13801 tgatcgacga tggcgtcaat ttcgttaatc aaaccagcca gacctttgat gtcattatct
    13861 ccgactgcac cgatcctatc ggtcccggcg aaagtctgtt cacctcagca ttttatgaag
    13921 gctgcaaacg ttgcctgaat cctagcggta tcttcgtcgc acaaaacggc gtctgctttt
    13981 tacagcagga agaagccatc gacagccatc gcaaactcag ccattacttc agcgacgttg
    14041 gcttttatca ggcggcgatc ccgacctatt acggcggtat catgactttt gcatgggcga
    14101 cagataacga cgccttacgc catctctcaa ccgaaattat tcaggcgcgt tttctcgctt
    14161 ctggcctgaa atgccgttat tacaatccgg caatccatac ggcagctttt gccttacctc
    14221 agtatctgca agatgcactg gcttcacagc cgtcctaagg agaagataag aaattgaaaa
    14281 aactgaaact gcatggcttt aataatctga ccaaaagtct gagtttttgt atttacgata
    14341 tctgctacgc caaaactgcc gaagagcgcg acggttatat tgcttatatc gatgaactct
    14401 ataatgccaa ccgtctgacc gaaatcctgt cagaaacctg ttccattatc ggggccaata
    14461 ttcttaacat cgcccgccag gattacgaac cacagggtgc cagcgtcact attctggtga
    14521 gtgaagaacc ggttgacccg aaactcatcg acaaaacaga acaccccggc ccactgccag
    14581 aaacggtcgt tgcccatctc gataaaagtc atatttgcgt acatacctac ccggaaagcc
    14641 atcctgaagg tggtttatgt accttccgcg ccgatattga agtctctacc tgcggcgtga
    14701 tttctccgct gaaggcgctg aattacctga tccaccagct tgagtccgat atcgtaacca
    14761 ttgattatcg cgtgcgcggt tttacccgcg acattaacgg tatgaagcac tttatcgacc
    14821 atgagattaa ttcgattcag aactttatgt ctgacgatat gaaggcgctg tatgacatgg
    14881 tggatgtaaa cgtctatcag gaaaatatct tccataccaa gatgttgctt aaagagttcg
    14941 accttaagca ctacatgttc cacaccaaac cggaagattt aaccgacagc gagcgccagg
    15001 aaattaccgc tgcgctgtgg aaagaaatgc gcgagattta ttacgggcgc aatatgccag
    15061 ctgtttaacg gctctggcgg agctccaggc tccgccagat ttatttactt ctgctgcacg
    15121 aaattgcggt aagccgccac gacctgcaga aaatcctcaa cgccgcatag cgacaggctt
    15181 tcttcgtcgt agtagttcat cccctcttcc atttcatcgc cagcgaattc cagttgattg
    15241 gcgcgaacca tcacctcttc accgtccatc cacagggtgt attcatgccc tgcccgttgc
    15301 caggaccgtt cgctaccttt cagtgcgtgc gctgcttgtt ccacttcatc aagcaaggcc
    15361 aggttttctt tcacctcttc attaaaccag tgcccgacca cttcatgccc catggacata
    15421 cgcaccttta ccactccggt aatatcgcgc agaaattcgt aatccatagt gtgttcctct
    15481 gctcccggca atgcgccgaa atcgcattgc gccattgatg taattatcgc agccttaccg
    15541 gagaaaaaca gcggaacggc gatggtttag aaataaaaaa tgcccgggaa tctcattcac
    15601 cgggcattgt gtcgtttatg cgcagcgcgt gcgcttactt attacaccgc agtctggaaa
    15661 atcaccccat cggctttttc ggtgtactga gaaagttggt tgaagttcag ataacgataa
    15721 gtatcaacgg cagttttatc tacttgcgca acgtaggtct ggtactcttc cggcgtcggc
    15781 agtttgccaa tcagcgccgc aacagccgcc agttctgcag aagccaggaa gacattcgcg
    15841 ccagtcccca gacggttcgg gaagttacgg gtagaggtgg aaaccaccgt cgcaccgtct
    15901 gctacacgcg cctggttacc catacacaga gaacagcccg ggatctcgat acgagccccg
    15961 ctcttaccga agacgctgta gtagccttct tcagtcaact gtgcggcgtc catacgggtt
    16021 ggcggtgcca cccacaggcg ggtcggcaac tggcctttat gcgcatccag cagtttaccc
    16081 gcagcacgga agtgaccgat gttagtcatg caagaaccga taaatacttc gtcgatcttc
    16141 tcgccctgta ccgcagacag cggacgcgcg tcatccgggt cgttcggagc acacaggatt
    16201 ggctctttaa tatccgccag atcgatgtca atcactgccg cgtattccgc atctgcatct
    16261 gcttccagca gctcaggatt cgccagccat ttttccatgc cctgaatacg acgttccagg
    16321 gtacgacgat cgccgtaacc ttccgcgatc atccacttca gcaggacgat attagagtta
    16381 aggtattcga tgattggttc tttgttcagc ttgatagtac aaccagcggc agaacgctca
    16441 gcggacgcat cggtaagctc aaaggcctgc tcaactttca gatccggcag accttcaatt
    16501 tccagaatgc ggccagagaa gatgtttttc ttgcctttct tctcaacggt cagcagacct
    16561 tgtttgatgg catacagcgg aatggcgtgt accagatcgc gcagggtgat gcccggctgc
    16621 attttgcctt tgaagcgcac cagaacggat tccggcatat ccagcggcat tacgcctgtt
    16681 gcggctgcaa atgccaccag accagaaccc gccgggaaag agataccgat cgggaaacgg
    16741 gtatgggagt cacctccggt accgacggta tccggcagca gcatacggtt cagccaggag
    16801 tggattacgc cgtcacccgg acgcagcgac acaccgccac ggttcataat gaagtccggc
    16861 agcgtgtggt gcgtgttcac gtcaactggc ttcggatacg ccgcggtgtg gcagaaagac
    16921 tgcatcacca ggtcagccga gaagcccagg cacgccaggt ctttcagttc atcacgggtc
    16981 atcgggccgg tagtgtcctg tgaacctaca gaagtcattt tcggttcgca gtacgcgccc
    17041 ggacgaatgc ctttcacgcc acaggcacgg cctaccattt tctgcgccag cgagaagccg
    17101 cgatcgctct cagcgacatc tttcgcctga cggaacacat cactgtgcgg cagaccaagt
    17161 gcttcacgcg ctttggtggt caggccacgc ccgataatca gcgggatacg gccgccagca
    17221 cgcacttcat caatcagcac gtcggttttc agttcgaagg tcgccagcag ttcgcctgtt
    17281 tcgtggttac gcacttcacc tttgtacggg taaacgtcaa tcacgtcgcc catgttcagg
    17341 ttagagacgt cgacttcgat tggcagtgca cccgcgtctt ccatcgtgtt aaagaagatg
    17401 ggtgcaattt taccgccgag gcacaaacca ccgccgcgtt tgttcggcac atgtggaata
    17461 tcatcgccca taaaccacag aacggagttc gtggcggatt tacgcgatga acccgtaccc
    17521 acaacgtcac cgacgtacgc cagcgggaaa cctttctgtt gcagagcttc aatctgtttg
    17581 atcgggccaa caacacctgg ctggtctggc tcaatacctt cacgggcgtt tttcagcatc
    17641 gccagcgcgt gcagtgggat atccgggcgc gaccacgcat ccggtgccgg agaaaggtca
    17701 tcggtgttgg tttcgccagt gactttgaag acggtaacgg tcagtttttc agccagcgcc
    17761 gggcgattca gaaaccattc ggcatccgcc caggactgca ttacctgctt cgcatattcg
    17821 ttgcctgctt tcgctttctc ttctacgtca tagaagttat cgaacatcag cagtgtgtga
    17881 gaaagtgctt tggcagcgat cggtgccagt ttggcatcat ccagcgcgtc gatcagcgga
    17941 tgaatgttgt aaccaccctg catggtgccc agcagttcga tggctttttc cggagtcagc
    18001 agaggggatt tggcttcgcc tttcgcgata gcagccagga agcctgcttt gacataggcg
    18061 gcttcatcga cgccaggggg aacacggttg gttaacagat ctaacaggaa ttcttcttcg
    18121 cccgcgggcg ggtttttcag cagctctaca agtgcggcca tttggtttgc atccaggggt
    18181 ttgggcgcaa tcccctcagc ggcacgctca gctacgtgct tacggtattc ttctagcacg
    18241 acggttctcc tcgctctcat tgtcatagtg cggcaggcga ttctcttcac gctcctgtga
    18301 gacagcagtt tgtagggtaa atgcccagta ccgcaacggg cagaatagca ggattttgac
    18361 gacgtgttaa tctgtttaca aaaaagcaac ataaaaaagt ggctgaatcg ttaaggatgg
    18421 tctagagatt gtttccctcc gtaaaaactt cgttaattcg catggtgata atcacctttc
    18481 tcaacgaatc ccaagattag tcaaaattta aactaccgcc tctttatact cggattaaca
    18541 gcacctgcgg gtggcagttc gccgaccatt gcgatttcct tgagatccga attatgaaaa
    18601 tgactttgcc gtttaaaccc catgtgctgg cactaatttg cagtgccggg ctttgtgccg
    18661 cctctgccgg gctatatata aaaagccgca cagtggaagc ggctgtggaa acgcaatcga
    18721 cacaactggc tgtgtctgac gctgccgcgg ttacgcttcc tgcaacggtt tccgcgcctc
    18781 ccgtaacacc cgccgtcgtt aaatccgcat tcagcactgc acaaatagat caatgggtcg
    18841 cgcccgtcgc gctgtatccc gacgccctac tttcgcaggt gctgatggca tcaacctatc
    18901 cggcaaacgt tgctcaagca gtgcaatggt cgcacgataa tccacttaaa caaggcgatg
    18961 ctgctattca ggcggtatct gaccagccgt gggacgccag cgttaaatca ctggtggcct
    19021 ttccacaatt gatggcattg atgggcgaaa acccgcaatg ggtgcaaaac ctgggcgatg
    19081 cttttctggc gcagccgcag gacgtgatgg actcggtaca gcgattgcgg caactggcgc
    19141 aacaaaccgg ttcgctgaag tcatcaaacg aacaaaaagt tattaccaca acgaagaaag
    19201 ctgtaccggt aaaacagaca gtcacggcac ccgtcatacc atccaatacc gttttaactg
    19261 ccagccccgt cattacagag cctgcaacaa ccgtcatttc cattgagccc gccaatcctg
    19321 atgtggtcta tattcccaac tacaacccaa ccgtggttta cgggaactgg gccaatactg
    19381 cgtatccgcc ggtttatctg ccaccaccag ccggagaacc gtttattgac agctttgtgc
    19441 gcggattcgg ctatagcatg ggtgttgcta ccacgtacgc actattcagc agcatcgact
    19501 gggatgacga cgatcatgac catcatcatc atgacgatga tgattatcat caccacgatg
    19561 gcggtcatcg tgacggtaat ggctggcaac acaacggcga caacatcaat atcgacgtca
    19621 acaatttcaa ccgtatcacc ggtgagcatc ttactgataa gaatatggca tggcggcaca
    19681 atccaaacta ccgtaatggt gtgccctatc atgatcagga tatggcaaag cggtttcatc
    19741 aaaccgatgt caacggcgga atgagtgcca cgcagctacc tgctccaaca cgcgacagcc
    19801 agcgtcaggc ggcagcaagt cagtttcagc aacgaacaca cgccgccccc gtcattacac
    19861 gagataccca acgtcaggca gcggcacagc ggtttaatga agctgaacac tatgggagct
    19921 atgacgactt ccgcgacttc agccgtcgcc aacccctgac ccagcaacaa aaggacgccg
    19981 ctcgtcagcg ttatcagtca gcttctcctg agcagcgcca ggcagttcgc gagagaatgc
    20041 agactaaccc gcagatccag cagcgaagag aggcagcgcg tgagcgcatt cagcccgcct
    20101 cgcctgagca gcgccaggca gtccgcgaga aaatgcagac taacccacag atccagcagc
    20161 gaagagacgc agcgcgtgag cgtattcagt cagcctcgcc tgagcagcgc caggtgttta
    20221 aggaaaaagt acagcagcgc ccactgaacc aacagcaacg tgataacgcc cgccagcgtg
    20281 ttcaatcagc atcacctgaa caacgtcagg tttttcggga gagagttcag gagagccgcc
    20341 cacaacgtct aaacgacagt aaccgtactg ccagattgaa taacgatcaa cggtcagcag
    20401 tacgcgaacg tctctctgag cgcggagcaa ggcgactgga aaggtaaatt acagacgtaa
    20461 aaaaagcggc gtggttagcc gcttttttaa ttgccggatg ttccggcaaa cgaaaaatta
    20521 cttcttcttc gctttcgggt tcggcaggtc ggtaatgcta ccttcgaaca cttctgccgc
    20581 caggcccaca gactcgtgca gagtcgggtg cgcatggatg gtcagcgcga tgtcttcagc
    20641 gtcacaaccc atttcgattg ccaggccgat ttcacccagc agctcgccgc cgttggtacc
    20701 gacaatcgca ccaccgatca cacggtgaga ttctttgtcg aaaatcagct tggtcatacc
    20761 gtctgcgcag tcggaagcga tagcacgacc agaagcagcc cacgggaagg tggcggtttc
    20821 atagctgatg cctttctctt tcgcttcttt ctcagtcaga cctacccatg caacttctgg
    20881 ttcggtatag gcgatagacg ggataacttt cggatcgaag tagtgttttt taccggcgat
    20941 aacttcagcg gcaacgtgac cttcgtgaac acctttgtgt gccagcatcg gctgaccaac
    21001 gatatcgccg atagcaaaga tgtgcggtac gttggtacgc agctgtttgt caacgcggat
    21061 gaaaccacgg tcgtccactt ccacgccagc tttgcctgcg tcgaggtttt taccgttcgg
    21121 cacacgacca atcgctacca gcacggcgtc gtaacgctgc ggttcagcgg gtgctttttt
    21181 gccttccatc gtcacataaa taccgtcttc tttcgcttca acggcggtaa ctttggtttc
    21241 cagcatcagg ttgaatttct tgctgatacg cttggtgaag actttaacga tgtctttgtc
    21301 agctgccggg ataacctggt cgaacatttc aaccacgtca atctgtgaac ccagcgcgtg
    21361 gtatacggtg cccatttcca gaccgatgat accgccaccc attaccagca ggcgttctgg
    21421 tacttctttc agttccagcg cgtcagtgga gtcccagata cgcggatctt catgcggaat
    21481 aaacggcagt tggatcgggc gagaacccgc tgcaatgatc gcgttgtcga agttgatcac
    21541 ggttttaccg ttctcacctt caacttccag ggtgttagcc ccggtaaatt tacccagacc
    21601 gttgaccact ttgactttgc ggcctttcgc cataccagcc agaccaccgg tcagctgatt
    21661 gatcactttc tctttccagg tacgaatctt gtcaatatcg gttttcggtt cgccgaagac
    21721 gataccgtgt tcagccagcg ctttggcttc ttcgataact tttgctacgt gcagcagtgc
    21781 tttagaaggg atacagccga cgttcaggca aacaccgcca agggtgttgt aacgttctac
    21841 gattacggtt tccagaccta aatcagcgca acggaaggca gcggagtaac ctgcggggcc
    21901 tgccccaagt accacgacct gagttttgat ttcagtactc atcatgacct ctatatattt
    21961 atctccggcg gtcatacccg tcgtctttca ggccgcagcg gcgttagctt cacgccctca
    22021 ccccagtcac ttacttatgt aagcccctgg ggattctggc gcttgctacc ttgctgcacc
    22081 cagaaatcca tagggtatgt gaagtaatac cctaaccacc accgggtcgt tctatccgtc
    22141 ggtattttac aaaattgtta acaattttta aacaacaaac ggcaaccgat ttgtctattc
    22201 gctaataacc tcaatacatt catgagatta ccagaaaaaa gccggccgtt gggccggctc
    22261 ttttacttac atcaccagac ggcgaatgtc agacagcgtg ttgttaatga tggtaatgaa
    22321 acgggcacca tcagcaccgt cgatcacgcg gtggtcgaag gagagagaaa tcggcagcat
    22381 cagacgcggc acgaactctt taccattcca caccggctcc atcgcggact tggaaacgcc
    22441 gaggatagcc acttccggcg cgttcacaat cggcgcgaag tgggtagtac ccaggccgcc
    22501 gatgctggag atggtgaagc aaccgccctg catttcgccc gcagtcagct taccgtcacg
    22561 cgctttctta gaaatagtca tcagctcgcg agacagctcg atgatgcctt tcttgttgac
    22621 gtctttgaat accggaacaa ccagaccgtt cggggtatcc accgccacac cgatgttgat
    22681 gtatttcttc agggtcagac gctgaccgtc ttccgacagc gaactattga agcgaggcat
    22741 ctgctcaaga gctgcagcaa cggctttcat gatgaagaca accggggtga tcttcacatc
    22801 cagcttacgt ttcgccgctt cttcgttctg ctgtttacgg aacgcttcca gctcggtgat
    22861 atcggttttg tcgaagtggg taacatgcgg gatcatcacc cagttacggc tcaggttagc
    22921 accagaaatt ttctggatac ggcccagttc cacttcttcg atttcaccaa acttgctgaa
    22981 gtccaccttc ggccacggca gcatgcctgg gataccgccg ccagtcgccg ccggagctgc
    23041 ttctgcacgt ttgatagctt ctttcacgta agcctgaacg tcttcgcgca ggatacgacc
    23101 tttacggcca gtgcccttca ctttcgccag gttaacacca aactcgcgtg ccagacggcg
    23161 gatcagcgga gtcgcgtgaa cataagcgtc gttttcagca aattcagatt tgccttccgc
    23221 tttcgcagct ggtgctgctg ccggggcttc agcttttgct gcctgtgccg gcgctgccgc
    23281 ttcctgtttc gcaggagctg ccgcaggcgc tgcgccttca acttcgaaga tcataatcag
    23341 cgagccagtt ttcactttat cgccaacgtt gactttcagt tccttcacga cgcctgcaaa
    23401 cggcgccgga acttccatag aagctttgtc gccttctacg gtgatcagtg actgttcagc
    23461 ggcaactttg tcgcccactt tcaccatcac ttcagtcact tcaacttcgt caccgccgat
    23521 atccggaacg ttaacttctt tcacgccagc tgctggtgca ggggccgctg ccggagccgc
    23581 ttcctgttta gccgccggag ctgccgcgcc tgcttcaccc gcgacttcga agaccataat
    23641 cagcgagccg gtagacactt tgtcacccac gttcactttg atctctttca cggtgccagc
    23701 aaacggagcc ggaacttcca tagaagcttt atcgccttct acggtgatca gcgactgttc
    23761 agcttcaact ttatcgccaa ctttcaccag gatttcggtc acttcaactt cgtcgctgcc
    23821 gatatccgga acgttaacgt cttttgccgc cgcagccgcg ggtgctgctg ccggagctgc
    23881 ttctttcttc tcttctgcct gagcaggtgc agcgtctgct gcaccgtcgg cggaatcgaa
    23941 aatcataatc agtgcgccgg tctgggtttt atcgccaaca gagactttga tctctttaac
    24001 gatacccgcc tgcggagacg gaacttccat agaggctttg tcgccttcta cggtgatcag
    24061 cgactgttcg gcttcaactt tgtcgcccac tttgaccagg atctcggtga tttcaacttc
    24121 atcagccccg atgtccggta ctttgatttc gatagccatt attcttttac ctcttacgcc
    24181 agacgcgggt taactttatc tgcatcgatg ttgaatttgg cgattgcgtc agcaaccact
    24241 ttcttatcga tttcgccacg tttagccagt tcgcccagcg ccgcaaccac gacataagaa
    24301 gcatcaactt cgaagtggtg acgcaggttc tcacggctgt cggaacgacc gaagccatca
    24361 gtacccagta cgcggtagtc gtcagccggt acgtaagtac ggacctgctc agcgaacagt
    24421 ttcatatagt cggtagatgc cactgccgga gcgtcgttca tcacctgagc gatatacgga
    24481 acgcgcggag tttccagcgg atgaagcatg ttccagcgtt cacaatcctg accatcacgc
    24541 gccagctcgg tgaaggaggt cacgctataa acgtcagaac ctacgccgta atctttcgcc
    24601 aggatctctg ccgcttcacg gacgtgacgc aggatagaac cggagcccag cagctgaact
    24661 ttacctttgc taccttcaat ggtttcgagt ttgtagatac ctttacggat accttcctca
    24721 gcaccttccg gcattgccgg catgtggtag ttttcgttca gcgtggtgat gtagtagtaa
    24781 acgttctctt gtttttcacc gtacatacgc tccagaccgt catgcataat gacagcaact
    24841 tcgtaagcgt aagccgggtc gtaagagata cagttcggaa tagtcagcga ctgaatgtgg
    24901 ctgtgaccat cttcatgctg cagaccttct ccgttcaggg tggtacgacc ggaagtaccg
    24961 ccgatcagga agccacgcgc ttgctggtcg ccagccgccc agcacagatc gccaatacgc
    25021 tggaagccga acatcgagta atagatgtag aacgggatca tcggcagatt gttggtgctg
    25081 taagaggtcg ccgctgccag ccaggaacaa cctgcgccca gctcgttgat cccttcctgc
    25141 agaatctgac ctttctcatc ttctttatag taagcaacct gctcgcggtc ctgcggggtg
    25201 tactgctgac cgttcgggct gtaaatacca atctgacgga acagaccttc cataccgaaa
    25261 gtacgcgctt cgtcggcgat gatcggtacc agacgatctt tgatcgactt gttcttcagc
    25321 attacgttca gggcacgaac gaaagcgata gtggtagaga tctctttgct ctgctcttcc
    25381 agcagcgcgc cgaagtcttg caggctcggc agctcaagct tctcggtgaa gttcggctga
    25441 cggcttggca gataaccgtg cagtttctga cgctgtgcgt gcagataggt atgctcttca
    25501 gaaccttccg ggaaggtgat gtacggcagt ttttcgatat ctgcatcaga caccggcaca
    25561 ttgaaacggt cgcggatgtg acgcacgccg tccatgttca ttttcttaac ctggtgcgcg
    25621 atgtttttac cttcagccgc gtcgcccatg ccgtaacctt taatggaatg agcaaggatt
    25681 actgtcgctt tgcctttggt ttcctgcgct ttcttgaatg cagcgtagat tttcttcgga
    25741 tcgtgaccac cacggttcag tgcccagatc tgctcgtcag tccagtctgc aaccagtgct
    25801 gcggtttcag gatatttacc gaagaagtgt tcacgaacgt acgcaccatc tttcgatttg
    25861 aaggtctggt agtcgccgtc aacggtttcg ttcatcagct ggatcagttt accgctggta
    25921 tctttacgca gcagttcatc ccaacggcta ccccacatca ctttgatcac gttccagcca
    25981 gcaccttcga agatgccttc cagttcgttg atgatcttgc cgttaccggt gaccgggccg
    26041 tcaagacgct gcaggttaca gttgataacg aagaccaggt tatccagttt ttcacgggta
    26101 gcgatggtga tcgcaccttt ggattccggt tcgtccattt caccgtcgcc gaggaacgcg
    26161 taaacggtct gtttagaggt atctttcagg ccacggtgtt ccagatattt caggaattta
    26221 gcctggtaaa tagcaccaat cggccccagc cccatagata cggtcgggaa ctgccagaat
    26281 tccggcatca gtttcgggtg cggataggaa gagaggccat tgccgtgaac ttcctgacgg
    26341 aagttatcca gctgctcctg agtcagacga ccttccagga aagcacgagc gtacacgccc
    26401 ggggagatgt ggccctggaa gtaaaccagg tcgccgccat cctgctcgtt gcgtgcacgg
    26461 aagaagtggt taaagcacac atcataaatg gttgcggaag actggaagga cgccatatgg
    26521 ccgcccagtt cgaggtcttt tttcgacgca cgcagcaccg tcatgatggc gttccagcgg
    26581 atagctgaac gaatacggcg ttccagttcc agattacccg gatactccgg ttgttcttca
    26641 acggggatgg tgttgatgta gttgctgata cctgtgcctg cggctacgtt tacaccgcct
    26701 ttgcgggctt cagcaagcag ttggtcgatc agatactgag cacgctcaac accttcttca
    26761 cggatgaccg attcgatcgc ctggagccag tcgcgagttt cgatcggatc cacgtcattt
    26821 gggaaacgtt ctgacatggg ttattcctta tctatctaat aacgttgagt tttctggaac
    26881 ctgtcccatt gaactctcgc cggaaagctc aataagacag gttctacgtt tagttgccgc
    26941 gcttttatat gcgcttgatt tacaacatct tctggataat ttttaccagt aaaatcacta
    27001 attctttcgt tgctccagac gacgcagaga acgctcacgg cggctctctt cacgacttct
    27061 gtcgagcaaa atttcttcga taaaggccag atggcgatgc gatgcttcgc gcgcttcttc
    27121 cggcttaccg gccataatcg cttcaaatat gcgggtgcgg tgactactca ccagcggcag
    27181 catctcgcga cgcgaataga gcaattcgaa gttctggcgg acattctggg ccaacatcgg
    27241 ctccatacac cttagcagat gaagcagaac cacattgtgg gccgcttcag tgacagcaat
    27301 ctgatactgg agtacggcgt ttgattccgc gtccagatcg ccagactgct gcgccagttc
    27361 tatggcgtga tggagttcac ggatgcgttc cttgtcttca tcggtactac gcagcgcggc
    27421 gtaataagcg gcgatacctt ccagggcgtg tcgtgtttcg agcaagtcat actgtgactc
    27481 aggatggtcg gagagcagct ccaccagcgg atcgctgaag ctttgccata ggctgctctg
    27541 gacaaaagtg ccgccaccct ggcgacgaag caacaagccc ttcgcttcga gacgttgaat
    27601 cgcctcacgc aaggagggac gggagacgtc aaactgtttt gccagttcgc gttccggtgg
    27661 gagtttttcg cccgggcgga gagtgccttc gaggatcaaa aactccagtt gctgctcaat
    27721 cacatcggag agttttggtt ggcggatttt gctgtaggcc atgagttcct gtcttaagcc
    27781 acttgccgaa gtcaattggt cttaccaatt tcatgtctgt gacgctaaag taacaaagta
    27841 ttcaccttat gtccatacag gttttgattg aaatcatgaa actgtgcaca ttttaacaac
    27901 ttgacatata taacgtttca aagttgtaac tatgcacaaa tgtagacttt acgtaggaaa
    27961 gggatttttt aacgattatt aaactataaa aatccgaatt gaaccgattc acttaccaat
    28021 tttgtgattt ttaattcaat taaaacgaat ttaaattcat tctacatatt gagaggggtt
    28081 gaggctgagc tttacaaacg gtttcttttt aagcaactca tcttcaacca tgcataaagc
    28141 gggtgcattc gctgccgcat accattattc ttgatctgac ggaagtcttt ttgtaacaat
    28201 tcaaacttct ttgatgtaaa caaattaata caacaaacgg aattgcaaac ttacacacgc
    28261 atcactgcgt agatcaaaaa aacaaccacc gcacgaggtt tcatgatgga aggtcaacag
    28321 cacggcgagc agctaaaacg cggccttaaa aaccgccata ttcagcttat cgcgctgggt
    28381 ggcgcgatag ggaccgggtt attcctgggt agcgcctccg taatacagtc cgcagggcca
    28441 gggattatcc tgggttacgc cattgctggt tttatcgcct ttctgatcat gcgtcagctg
    28501 ggtgaaatgg tggtcgaaga acctgtcgca ggctccttta gccactttgc ttataaatac
    28561 tggggcagtt ttgccggttt cgcctctggc tggaactact gggtactgta cgttttagtt
    28621 gccatggctg agctgactgc cgtgggtaaa tacattcagt tctggtatcc ggaaatcccc
    28681 acctgggttt ctgccgccgt attctttgtg gtgattaacg ccatcaacct gaccaacgtt
    28741 aaagtgtttg gcgagatgga gttctggttt gccattatca aagttatcgc tgtggtagcg
    28801 atgatcatct tcggcggctg gctgctattc agtggcaaca gcggcccgca ggcgaccgtt
    28861 agcaacctgt gggatcaggg tggtttcctg ccgcacggct tcaccgggct ggtgatgatg
    28921 atggcgatta tcatgttctc gttcggtggt ctggaactgg tggggatcac cgcagcagaa
    28981 gctgataacc cggagcaaag tataccgaaa gcaactaacc aggttatcta ccgcatcctg
    29041 attttctata ttggttcgtt agccgttctg ctctcactga tgccgtggac ccgcgttacc
    29101 gccgatacca gtccgtttgt gctgatcttc cacgagttag gcgatacctt tgtggcgaat
    29161 gcgctgaaca tcgtggtact gactgcggcg ctctccgtgt acaacagctg cgtatattgc
    29221 aacagccgta tgctgtttgg tctggcacaa cagggtaatg cgccaaaagc gctggcgtct
    29281 gtcgataaac gtggtgtacc agtaaatacc attctggtgt ctgcactggt aacggcgttg
    29341 tgcgtactga ttaactacct tgccccagag tccgctttcg gactgttaat ggcgctggtg
    29401 gtatctgcac tggtaatcaa ctgggcgatg attagcctgg cgcatatgaa attccgtcgc
    29461 gccaagcagg aacaaggcgt ggtaactcgc ttccctgctc tgctttatcc gctgggtaac
    29521 tggatctgcc tgctgtttat ggcggcggta ctggtgatta tgctgatgac cccaggaatg
    29581 gcgatttcgg tatacctgat cccggtatgg ctgatcgtgt taggtatcgg ctatctgttt
    29641 aaagagaaaa ccgccaaagc cgtaaaagcg cattaatctc tctacgccct cacccgtaca
    29701 gggtgagggc aataatcttt acaccaacgc cccgtaaatc gtcagtagtg caatcaccac
    29761 cacgaccacg aacgaggttt tcttcgccat tgaaaccgct gccttcggcg tctccacctt
    29821 atcgacatgc ggttcacgcg ccagagagaa ctgcgccaga cgcgttaaca cctgatactg
    29881 cgaagtatgg aagtcaccca gcgaagcaaa ccaggccggt aacgctttct ccccatgacc
    29941 gatcaaggca tataccacac ccgcaagacg aaccggcacc caatccagta catgaagcac
    30001 ggcatcaatg ccggactgta aacgatgatg cggcgtctgg tatcgcgcca gccagtattg
    30061 ccatgcacgc aaaaacgcat accccatcag cgtaacgggt ccccagattc cccccacaat
    30121 caaccagaac agcggagcaa gataaaaacg aaagttaatc cacagcaatg cattttgcag
    30181 ctcacgcaaa tactcacgtt catcgcagcc tgccgggacg ccgtgaatca tggtgagttc
    30241 gccagccatc gtgacacggg catggctatc attacgtgaa gcagctgtca gataagcatg
    30301 ataatgaaga cgaactttac ctgcgccaat acacagcaaa ccaatcagca gccacaccag
    30361 cagcgtggga acgttgaaca atactccctg caatgcgcgt aacagtaaaa aagtcacgcc
    30421 catcgcaata atggtcatgc ctaacgtgcg cccgagagaa aaatgtttca cccgccgaaa
    30481 gaacgcttca agacgatgat caagctgcca gtgctcgccc aacttaaaca ggcgctcgaa
    30541 aattaacacc agtaaggttg taaatagcgt catgttgtct ccttgctgac cagcgcacga
    30601 aaccgcgccc aatcaaatgc agggccggga tcggttttcc gatccggcgc aatatcacaa
    30661 tggcccgtca tgttattagc gatatctgga tagcgatcaa tcagtgcccg cgtaaccgcc
    30721 gcaagctgtt gatactgcgc atcggtatac gccagcgtat cggtgccttc aagctcaatc
    30781 ccaatagaaa aatcattgca gcgttcgcgc ccctgatact gagagactcc cgcatgccat
    30841 gcacgtttat cgaaaggaac atactggact atttcaccat cacggcgaat caaacagtga
    30901 gcggagacgc gcaaatgggc gatctcagca aagaaaggat gtgcctgcgg atcaatagtt
    30961 ccagtgaata atgcgtcgat ccacggaccg ccaaactcgc ctggcggcag gctaatattg
    31021 tgcaccacca gcagggtggg tgtttcgtca tccgggcggc aatcgtaatg tggtgaggga
    31081 acgcggcgcg cgccaaccag ccacccctgt tctaacaaca tgcaggatct ccttatatgt
    31141 ggtgctaata cccggttcag agtagcatgt ttctacctta tgattcgtta gctatctgga
    31201 gttttaacat gccgcctcgc cgctataacc ctgacacccg acgtgacgag ctgctggaac
    31261 gcattaatct cgatatcccc ggcgcggtgg cccaggcgct gcgggaagat ttaggcggaa
    31321 cagtcgatgc caacaatgac attacggcaa aacttttacc ggaaaactct cgctctcatg
    31381 ccacggtgat cacccgcgag aatggcgtat tttgcggcaa acgctgggtt gaagaggtgt
    31441 ttattcaact ggcaggcgac gatgtcacca taatctggca tgtggatgac ggcgatgtca
    31501 tcaacgccaa tcaacccttg ttcgaacttg aaggcccatc ccgcgtgctg ttaacgggcg
    31561 aacgcactgc gcttaatttt gtgcaaaccc tttcaggagt tgccagtaag gtacgccact
    31621 atgtcgaatt actggaaggc accaacacgc agttgttgga tacgcgcaaa accttacctg
    31681 gcctgcgttc agctctgaaa tacgcggtac tttgcggcgg cggagcgaat caccgtctgg
    31741 ggctttctga tgccttcctg atcaaagaaa atcatattat tgcctccggc tcagtgcgcc
    31801 aggccgtcga aaaagcgtcc tggctgcacc cggatgcgcc agtagaagtc gaagtagaga
    31861 atctggaaga acttgatgaa gccctgaaag caggagccga tatcatcatg ctggataact
    31921 tcgaaacaga acagatgcgc gaagccgtca aacgcaccaa cggcaaggcg ctactggaag
    31981 tgtctggcaa cgtcactgac aaaacactgc gcgaatttgc cgaaacgggc gtggacttta
    32041 tctccgtcgg tgcgctaact aaacacgtac aagcactcga cctttcaatg cgttttcgct
    32101 aattgctgac agatttatcc gctccatcgc gggcggataa tctccccttc cccaactttc
    32161 ttcgtaacgc ctcgcaaatt tcttgtcttt cgctgcaacg aaataaaaaa cctcggaagc
    32221 tgctttcccg tttcgttttt tgcccctcgc ttgtgctgat ggcgtttgcc aaagtagcgc
    32281 caaccaaatc aaggagcgaa acagatggac aagcaacgcg gttttacact tatcgaactg
    32341 atggtggtta ttggcatcat tgccatttta agcgccattg gtattcccgc ttatcaaaac
    32401 tacctgcgca aagctgcact caccgacatg ctacaaacct ttgtgcctta ccgtaccgcc
    32461 gtagagttgt gcgcgctgga acatggtg
//
LOCUS       DAZPEB010000024        31674 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZPEB010000024 DAZPEB010000000
VERSION     DAZPEB010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31674
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.024"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            90..194
                     /locus_tag="ACHURJ_001005"
     CDS             90..194
                     /locus_tag="ACHURJ_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1280052.1"
                     /translation="MENNSRTMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            452..2650
                     /gene="speF"
                     /locus_tag="ACHURJ_001006"
     CDS             452..2650
                     /gene="speF"
                     /locus_tag="ACHURJ_001006"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HFR1280053.1"
                     /translation="MSKLKIAVSDSCPDCFTTQRECIYINESRNIDVAAIVLSLNDVT
                     CGKLDEIDATGYGIPVFIATENQERVPAEYLPCISGVFENCESRREFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEALFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     TKEARPFRLAVIQLGTYDGTIYNARQVVDKISHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGFDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQPQKEMFRKEHFPRV
                     SMNPQEANYAYSRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2647..3985
                     /gene="potE"
                     /locus_tag="ACHURJ_001007"
                     /pseudo
     CDS             2647..3985
                     /gene="potE"
                     /locus_tag="ACHURJ_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
     gene            4061..4210
                     /locus_tag="ACHURJ_001008"
                     /pseudo
     CDS             4061..4210
                     /locus_tag="ACHURJ_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
     gene            4334..4984
                     /locus_tag="ACHURJ_001009"
     CDS             4334..4984
                     /locus_tag="ACHURJ_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2625 domain-containing protein"
                     /protein_id="HFR1280054.1"
                     /translation="MREMNQLVNTEDSAWPIIQNWLKDATNHTELLPVNKNLAETALY
                     QLQVTTKSPMGALVYGSGGLLIDNGWLRIAGSGHPRLPRDPVSWTQRPEFAGVRALPI
                     ADDVAGGIFALNGGDLGEDTGCVYYFAPDTLNWESLEVGYSEFLQWALSGDLDTFYEN
                     VRWQQWREDVIKLSATEAFTFYPFLWVQSEEARTRKVISLTELWEMQYQMKETFTQ"
     gene            complement(5025..5519)
                     /locus_tag="ACHURJ_001010"
     CDS             complement(5025..5519)
                     /locus_tag="ACHURJ_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1280055.1"
                     /translation="MKTNRSLVVIVSLITATLLLTACAQPEQSSLAGDWLLTPKDKTR
                     RLTGSIAVNIAPSRCKTNCRGDNLPDNTRRWQLSGGNEKELTYLHNMSAQEKVGLNPG
                     WQCYTSFFMRVCQGKPGTRPIVNEDYVSESGFFGSMMHVGIIELRRCQSENCQQELKA
                     INTH"
     gene            complement(5734..7374)
                     /gene="pgm"
                     /locus_tag="ACHURJ_001011"
     CDS             complement(5734..7374)
                     /gene="pgm"
                     /locus_tag="ACHURJ_001011"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFR1280056.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALLADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(7400..7945)
                     /gene="seqA"
                     /locus_tag="ACHURJ_001012"
     CDS             complement(7400..7945)
                     /gene="seqA"
                     /locus_tag="ACHURJ_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFR1280057.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIAEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            8130..8894
                     /gene="ybfF"
                     /locus_tag="ACHURJ_001013"
     CDS             8130..8894
                     /gene="ybfF"
                     /locus_tag="ACHURJ_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR1280058.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     RDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPSGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            9034..9327
                     /gene="ybfE"
                     /locus_tag="ACHURJ_001014"
     CDS             9034..9327
                     /gene="ybfE"
                     /locus_tag="ACHURJ_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFR1280059.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            9467..9997
                     /gene="fldA"
                     /locus_tag="ACHURJ_001015"
     CDS             9467..9997
                     /gene="fldA"
                     /locus_tag="ACHURJ_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFR1280060.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            10207..10293
                     /gene="uof"
                     /locus_tag="ACHURJ_001016"
     CDS             10207..10293
                     /gene="uof"
                     /locus_tag="ACHURJ_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFR1280061.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            10286..10732
                     /gene="fur"
                     /locus_tag="ACHURJ_001017"
     CDS             10286..10732
                     /gene="fur"
                     /locus_tag="ACHURJ_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFR1280062.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(10817..11143)
                     /gene="chiQ"
                     /locus_tag="ACHURJ_001018"
     CDS             complement(10817..11143)
                     /gene="chiQ"
                     /locus_tag="ACHURJ_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFR1280063.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQHFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(11193..12599)
                     /gene="chiP"
                     /locus_tag="ACHURJ_001019"
     CDS             complement(11193..12599)
                     /gene="chiP"
                     /locus_tag="ACHURJ_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HFR1280064.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAVFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSLGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPAYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLLGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(13046..14710)
                     /gene="glnS"
                     /locus_tag="ACHURJ_001020"
     CDS             complement(13046..14710)
                     /gene="glnS"
                     /locus_tag="ACHURJ_001020"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFR1280065.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTRQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSSEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            14821..14943
                     /locus_tag="ACHURJ_001021"
     CDS             14821..14943
                     /locus_tag="ACHURJ_001021"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1280066.1"
                     /translation="MIDEKKSGREDLHRLSLLDATLKRRIRHKADYFLISYSGV"
     gene            complement(14913..16859)
                     /gene="nagE"
                     /locus_tag="ACHURJ_001022"
     CDS             complement(14913..16859)
                     /gene="nagE"
                     /locus_tag="ACHURJ_001022"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFR1280067.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPSTAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            17192..17992
                     /gene="nagB"
                     /locus_tag="ACHURJ_001023"
     CDS             17192..17992
                     /gene="nagB"
                     /locus_tag="ACHURJ_001023"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFR1280068.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            18052..19200
                     /gene="nagA"
                     /locus_tag="ACHURJ_001024"
     CDS             18052..19200
                     /gene="nagA"
                     /locus_tag="ACHURJ_001024"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFR1280069.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLARHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            19209..20429
                     /gene="nagC"
                     /locus_tag="ACHURJ_001025"
                     /gene_synonym="nagR"
     CDS             19209..20429
                     /gene="nagC"
                     /locus_tag="ACHURJ_001025"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFR1280070.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            20477..21229
                     /gene="nagD"
                     /locus_tag="ACHURJ_001026"
     CDS             20477..21229
                     /gene="nagD"
                     /locus_tag="ACHURJ_001026"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFR1280071.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETSSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            complement(21280..21378)
                     /locus_tag="ACHURJ_001027"
                     /pseudo
     CDS             complement(21280..21378)
                     /locus_tag="ACHURJ_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            21626..23290
                     /gene="asnB"
                     /locus_tag="ACHURJ_001028"
     CDS             21626..23290
                     /gene="asnB"
                     /locus_tag="ACHURJ_001028"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFR1280072.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSNQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            23671..23747
                     /locus_tag="ACHURJ_001029"
     tRNA            23671..23747
                     /locus_tag="ACHURJ_001029"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23705..23707,aa:Met,seq:cat)
     gene            23756..23840
                     /locus_tag="ACHURJ_001030"
     tRNA            23756..23840
                     /locus_tag="ACHURJ_001030"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23790..23792,aa:Leu,seq:tag)
     gene            23864..23938
                     /locus_tag="ACHURJ_001031"
     tRNA            23864..23938
                     /locus_tag="ACHURJ_001031"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23896..23898,aa:Gln,seq:ttg)
     gene            23973..24047
                     /locus_tag="ACHURJ_001032"
     tRNA            23973..24047
                     /locus_tag="ACHURJ_001032"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24005..24007,aa:Gln,seq:ttg)
     gene            24063..24139
                     /locus_tag="ACHURJ_001033"
     tRNA            24063..24139
                     /locus_tag="ACHURJ_001033"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24097..24099,aa:Met,seq:cat)
     gene            24188..24262
                     /locus_tag="ACHURJ_001034"
     tRNA            24188..24262
                     /locus_tag="ACHURJ_001034"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24220..24222,aa:Gln,seq:ctg)
     gene            24300..24374
                     /locus_tag="ACHURJ_001035"
     tRNA            24300..24374
                     /locus_tag="ACHURJ_001035"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24332..24334,aa:Gln,seq:ctg)
     gene            complement(24528..25703)
                     /gene="ubiF"
                     /locus_tag="ACHURJ_001036"
     CDS             complement(24528..25703)
                     /gene="ubiF"
                     /locus_tag="ACHURJ_001036"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFR1280073.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            25849..27273
                     /gene="miaB"
                     /locus_tag="ACHURJ_001037"
     CDS             25849..27273
                     /gene="miaB"
                     /locus_tag="ACHURJ_001037"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFR1280074.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGSTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            27426..28466
                     /locus_tag="ACHURJ_001038"
     CDS             27426..28466
                     /locus_tag="ACHURJ_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFR1280075.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLTIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            28463..28930
                     /gene="ybeY"
                     /locus_tag="ACHURJ_001039"
     CDS             28463..28930
                     /gene="ybeY"
                     /locus_tag="ACHURJ_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFR1280076.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            29020..29898
                     /gene="corC"
                     /locus_tag="ACHURJ_001040"
     CDS             29020..29898
                     /gene="corC"
                     /locus_tag="ACHURJ_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFR1280077.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            29923..31461
                     /gene="lnt"
                     /locus_tag="ACHURJ_001041"
     CDS             29923..31461
                     /gene="lnt"
                     /locus_tag="ACHURJ_001041"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFR1280078.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
BASE COUNT         7747 a         8083 c         8026 g         7818 t
ORIGIN      
        1 atcatttttt attgatcacc ttcacagttc aaccgtattt cctggataga atatcttcac
       61 cttcattcac atcaggaaag gtaaattaaa tggaaaataa cagccgcact atgccccata
      121 taaggcggac aactcatatt atgaagtttg ctcatcgcaa tagcttcgac tttcacttct
      181 tcaatgcccg ttagtctacc gactaagggc acttcagcgt acaggtcttc ctgactctct
      241 gtattacagg catatgcctg tattccgcag tgctcattct ctgttaaccc ttgtattatc
      301 agccaccgga ctggtaaggg tttctgcatt ctgaaaaaag caatctacct gatttattta
      361 tcagcggggt aactttgctt ttttccggca cgatcgattt ctcattcgag aaattgagga
      421 cctgctatta cctaaaataa agagatgaaa aatgtcaaaa ttaaaaattg cggttagtga
      481 ttcttgcccg gactgtttta ccacgcagcg agaatgtatc tacattaatg aaagtcgtaa
      541 tatcgatgtg gcggcaatcg ttttatcgct caacgatgtt acatgcggaa aactcgatga
      601 aatcgatgcc acgggttatg gcatcccggt atttattgct actgaaaatc aagaacgtgt
      661 acccgcagag tatttgccct gtatttcggg tgtctttgag aattgcgaat cgcgacgaga
      721 attttatggt cgtcagttag aaaccgctgc cagccattat gaaactcaac tgcgcccacc
      781 tttcttccgc gcactggtcg attatgtcaa tcaaggtaac agcgcgtttg attgccctgg
      841 tcatcagggc ggcgaatttt tccgtcgcca tccggcgggg aatcagtttg tggaatactt
      901 tggtgaggcg ctgttccgtt ccgacttgtg caacgcagac gtagcaatgg gtgatctgct
      961 gattcacgaa ggcgcgccat gcattgcaca gcaacatgcg gcaaaagtgt tcaatgccga
     1021 taaaacatac ttcgttctaa atggcacttc atcttctaac aaagtggttt taaacgccct
     1081 gctgacaccg ggtgatctgg tgctgtttga ccgcaataac cacaaatcta accaccacgg
     1141 agcgttgcta caggctggtg caacaccggt ttatctggaa accgcacgta acccgtatgg
     1201 ctttatcggt ggcattgatg cgcactgttt tgaagaaagt tacctgcgtg agctgatcgc
     1261 ggaagtcgca ccgcagcgga caaaagaggc tcgtcctttc cgcctcgccg ttattcagct
     1321 aggcacctac gacggtacga tttataacgc ccgccaggtg gtggataaaa ttagtcatct
     1381 gtgtgactac attctgtttg actccgcatg ggtcggctat gagcaattta tcccgatgat
     1441 ggctgactgt tcaccgctgt tgctggatct taatgagaac gatccgggca ttctggttac
     1501 gcaatctgtg cataaacagc aggctggttt ttctcaaact tcacaaatcc ataaaaaaga
     1561 cagccacatc aaagggcaac agcgttatgt accgcacaaa cgcatgaaca acgcctttat
     1621 gatgcatgcc tccaccagcc cgttctatcc gctgtttgcc gcactggata tcaacgccaa
     1681 aatgcatgaa ggtgtcagcg gtcgtaatat gtggatggat tgtgtggtaa atggcattaa
     1741 tgcccgcaag ctgatcctcg ataactgtca gcatattcgt ccgttcgtac ctgaactagt
     1801 ggatggtaaa ccctggcagt cgtatgaaac agcgcaaatt gcggttgatc tgcgcttctt
     1861 ccagtttgta ccaggggaac actggcactc ttttgaaggc tatgcagaga atcaatactt
     1921 tgtcgatcca tgcaaactgt tgctgacaac cccaggtttt gatgcacgta acggcgaata
     1981 tgaagcgttc ggtgtacccg cgacgattct tgctaacttc ctgcgcgaaa atggcgtagt
     2041 gccggaaaaa tgcgatctta actccatcct cttcctgctg actccggcag aagatatggc
     2101 caaacttcag caacttgttg ccctgctggt acgcttcgaa aaactgcttg agtccgacgc
     2161 gccattagca gaagtgctgc cttccatcta caaacagcat gaagagcgct acgccggtta
     2221 tacccttcgt cagttatgcc aggaaatgca tgatttgtat gcccgtcaca acgtgaaaca
     2281 accgcaaaaa gagatgttcc gtaaggaaca cttcccacgc gtaagcatga atccacaaga
     2341 agccaactac gcctattcac gcggtgaagt ggagctggtt cgtctgccgg atgccgaagg
     2401 ccgtatcgct gccgaaggtg cgcttcctta tcctccgggt gtgctgtgtg ttgttccggg
     2461 tgaaatctgg ggtggtgctg ttttgcgtta cttcagcgct ctggaagaag ggatcaacct
     2521 gctgccaggt tttgcaccgg agctgcaggg tgtctatatc gaagaacatg atggtcgtaa
     2581 gcaagtttgg tgctatgtca tcaagcctcg tgatgcgcaa agcaccctgt tgaaagggga
     2641 aaaattatga gtcaggctaa atcgaacaaa atgggcgtcg ttcagttaac catactgacg
     2701 atggtcaaca tgatgggctc cggtatcatc atgctgccga caaagcttgc cgaagttggg
     2761 acaatctcaa ttatctcctg gctggtgaca gccgtaggct caatggcact ggcatgggca
     2821 ttcgcaaaat gcggtatgtt cagccgtaaa tcaggcggta tgggcggtta tgcggagtat
     2881 gctttcggta agtccggcaa ttttatggcg aactacacct acggtgtctc gctgctgatt
     2941 gctaacgtcg cgattgctat ttcggcggtt ggttacggca ccgaactgct cggcgcgagt
     3001 ttgtcgccag tgcagattgg tcttgcgacc atcggagtgc tgtggatttg taccgtggct
     3061 aactttggtg gtgcgcgcat taccgggcaa atcagtagca ttactgtgtg gggagtcatt
     3121 attccggtcg tcggtctgtg cattattggc tggttctggt ttagtccgac gctgtacgct
     3181 aactcatgga acccgcacca tgtgccgttc tttagcgctg taggttcttc catcgccatg
     3241 acgttgtggg ctttcctcgg tctggagtca gcctgtgcca acgccgaagt agtggaaaac
     3301 ccggaacgta acgtgccgat tgcagttctt ggcggtactt taggtgcggc ggtgatttat
     3361 atcgtctcca ccaacgtgat tgccgggatt gtgcctaata tggagctggc aaattcaacg
     3421 gcaccatttg gcctggcttt cgcacagatg ttcacaccag aagtgggtaa agtcattatg
     3481 gcgctgatgg tgatgtcctg ctgcggttca ctgcttggct ggcggttcac tgcttggctg
     3541 gcagttcacc attgcccagg tgtttaaatc ttcatctgat gaaggctact tccctaaaat
     3601 tttctcccgt gtaaccaaag tggatgcacc ggtgcaggga atgttgacca ttgtgattat
     3661 tcagagtgga ttggcactga tgaccattag cccgtcactg aacagccagt tcaacgtgct
     3721 ggttaacctg gccgtggtga ccaatatcat tccgtatatt ctgtcaatgg cggcattagt
     3781 cattattcag aaggtcgcta atgtgccccc ctcaaaagcg aaagtagcaa actttgttgc
     3841 ctttgttggt gcgatgtaca gtttctatgc gctgtactca tccggggaag aagccatgct
     3901 gtacggttct atcgtgacct tccttggttg gacactgtat ggtctggtat caccatgctt
     3961 tgaactgaaa aataaacacg gttaataaaa caaagtaaaa aagggcgatt gaatgatcgc
     4021 cctttctgtt tgttggcaaa tagttcgtta tttatgccgc atccggcaga tgtagggtct
     4081 aaacgcgatc gaacacagca actatttgcg taattatatg gggcatctgc gacaaaaact
     4141 ggaacaggat ccggcccggc ctcgccattt tattactgaa accggtattg ggtatcggtt
     4201 tatgctttga aaaaagtggc aatgtatgga taaagtcatt ttttattcaa atataaggaa
     4261 cttaatattt aaaaaatgtt ccatacaatt cctcatcata aatgacaccg tagcacacaa
     4321 ggagcaagta gcgatgcgtg aaatgaatca gttagtaaat actgaagatt cagcctggcc
     4381 aataatccag aactggttaa aagacgccac aaaccatacc gaactcttac cggttaataa
     4441 aaatttagcc gaaaccgcac tttatcaatt acaggtgaca acaaaatcac cgatgggtgc
     4501 gcttgtctat ggctccggag gtctgctgat tgataatggt tggttacgca tagcaggatc
     4561 tggtcacccc cggttgccac gagatccagt aagttggaca caacgccctg aattcgcagg
     4621 cgtgcgggct ttacccatcg ctgacgatgt cgcaggggga atttttgctc ttaatggtgg
     4681 cgatcttggg gaagatactg gctgcgttta ttattttgcg ccagatacgc tgaattggga
     4741 aagtctggaa gttggatatt ctgagtttct tcagtgggca ttatccggtg atttagatac
     4801 cttctatgaa aatgtacgat ggcagcaatg gcgagaagac gtaataaaat tatcggcaac
     4861 ggaagccttc acgttctacc ctttcttatg ggtacaaagt gaagaagcaa gaacaagaaa
     4921 agttatttcg cttactgaac tctgggaaat gcagtatcaa atgaaagaaa cattcacgca
     4981 ataagacatg ttcaggtccg ggtttcattc ccggactcac tattttaatg tgtatttatc
     5041 gctttcaatt cttgctggca attttctgac tggcagcgtc gtaactcaat aatcccgaca
     5101 tgcatcatcg agccaaaaaa tccgctttcg ctgacataat cctcattgac tatcggtcga
     5161 gtgccgggct tgccctggca aacccgcata aaaaatgagg tgtagcattg ccaaccggga
     5221 tttaaaccga ccttttcttg tgctgacata ttatgaagat aagtaagttc tttttcgttc
     5281 ccgcctgaca actgccagcg gcgggtatta tcaggtaagt tatcaccacg gcaatttgtc
     5341 ttacaccggg aaggtgcaat atttaccgca atgctacccg ttaatcttct ggttttatct
     5401 ttaggggtca gtaaccagtc gccagctaat gatgattgtt caggttgggc gcatgcagtc
     5461 agcagtaatg tggcggtaat tagtgacact atgacgacca gactcctgtt ggtcttcatt
     5521 gggatttcct tgagctactc agtttgttga caaagtgttc gttgtttttg ccggatgcgg
     5581 cgtgaacgcc ttatccggcc tacaaaatct cgtaaattca atatattgca ccatcctcgt
     5641 aggcctgata agcgtagcgc atcaggcaat tctgtgtttg tcatacgtaa aaaagggcga
     5701 tcttccgacc gccctttttc ttatttaatg tgtttacgcg tttttcagaa cttcgctaac
     5761 aatctcaacc gcttctttct caatctgctt gcgatgttct tcaccgagga agctttcgca
     5821 gtagatctta tatgcgtctt ccgtgcctga cggacgcgcg gcgaaccagc cgttgtcagt
     5881 catcactttc agaccgccga tagaagcacc attgcccggt gccgccgtca ggcgcgcggt
     5941 gatcgggtcg cctgccaggg tgctggcgct caccatttcc ggagacagct tagacagcgc
     6001 cgctttttgt gcggaagtcg cagctgcctg caaacggttg tagctcggcg caccaaagcg
     6061 ttttgccagt tcgttgtagt gttcctgcgg gttcttaccg gtgacagcgg tgatttccgc
     6121 cgccagcaga cacatgatga tgccgtcttt gtcggtggac cacggcgtgc cgtcgaaacg
     6181 caggaaggaa gcccctgcgc tctcttcgcc gccaaagccg aagctgccgt cgaacagacc
     6241 atcaacaaac catttgaaac ctaccgggac ttctaccagc ttgcggccca agtcattgac
     6301 cacacggtcg atcatcgcag aagaaaccag cgttttaccg acagcaacat ctttgcccca
     6361 ctgcggacga tgctggaaca ggtagttgat cgccaccgcc aggtagtggt tcggattcat
     6421 caaacctgcc ggagtgacga taccgtgacg gtcataatcc gggtcgttag caaacgccag
     6481 atcgaactta tcacgcagtg ccagcaggcc cgccatcgca cactcggagg agcagtccat
     6541 acggatcgcg ccgtctttat caaggtgcat aaagcggaag gtttgatcga cctgatcgtt
     6601 aacgatggtc aggttgaggt tgtaatactc gccaatacgc ttccagtatt cgataccgga
     6661 accgcccagc ggatcaacgc ccagcgtcag gcccgctttc tgaatcgccg ccatatcaac
     6721 gatatcggcc agaccttcca caaacggctg caccagatcc tgctctttca catgaccgga
     6781 tgccatcgct tcgtcgaggg agatacgctt cacgcctttc aggccatcgg ccagcagtgc
     6841 gttggccctg tcttccacca ctttggtgac gttggtatca gccgggccac catttggcgg
     6901 attgtacttg ataccaccat cttccggcgg gttatgggac ggtgtaatca cgataccgtc
     6961 tgccagcgga ccaccttttt tattgtgaac caggatggca ttggaaacgg caggcgttgg
     7021 agtgaagcca ttgttttcct gcacaatgac atcaacgccg ttcgctgcca gcacttccag
     7081 tacggaaatg aacgcaggct cggacagggc gtgagtatct ttacccacat agcaagggcc
     7141 agtgatgccg tttttcgcac gttcttcagc aattgcctga gcgatcgcca gaatgtgcgg
     7201 ctcgttaaag ctgtggcgcg ctgcactgcc acggtgaccg gaagtaccga atttcaccgc
     7261 gtgctccgca ttccctgctt ctggtttcag tacataatat tgcgccgtca gttgggcgac
     7321 gttaatcaaa tcactctgtt gtgcaggttg gcctgcacga ttgtggattg ccattgcttt
     7381 gtcctttgtc tgcaacgttt tagatagttc cgcaaacctt ctcaatcaat tccgccggga
     7441 attgcatcga ctgcatgatg tgttcgatca tgctgcattt acggccggtg ttggtgttgg
     7501 tgatcaccca atacggcgtg cctggcacat gtttcggctt ggtctgatta ccatttttca
     7561 gcagcgtttg ttcatctgcc gcaaagtaaa cgcgtgtacg accgtgcaac gattccgttg
     7621 cttcggcaaa cgcctgggcg tcaagagaat atagtgtaga caacagcagc ataaagcgat
     7681 tgaccgctcg cttttgctct gcgtattcgt cagaaagcag aagttcacgc attgcgcgaa
     7741 ccttgtcttt aatcgttttg accggcttcg cttcggcgat agcaggtgac gcaacgcgaa
     7801 cctctttcgt caccggagca gcaggctgtg atgcggcgga aaatttcaac atacgccgta
     7861 aaatgtcgga tgcgctctcg ccgatatgct tagtgtggct ggcaatatag ctgtagagtt
     7921 catcatcaac ttcaatcgtt ttcatcttaa tccagtgcag tgtcttagct gaatacaagt
     7981 cgccaagagt atatgaataa atcccggcag cggatagcgt caagcctggc ttgcagaaaa
     8041 actcggaata aaccttattt gttgcagccc tgtggcacaa tggtcaacat aataccctaa
     8101 cccgacaacg cgaaaaaaag aactttgcca tgaaattgaa tatccgcgcg caaactgcac
     8161 aaaaccagca caataattct cccatcgttc ttgtccatgg tctgtttggt agcctcgaca
     8221 accttggcgt actggctcgc gatctggtaa acgatcacaa tatcatccag gttgatatgc
     8281 gtaaccacgg tctttcaccg agagatccgg taatgaatta cccggcgatg gcgcaggatc
     8341 ttgttgatac tctggatgca cagcagatcg acaaagcaac atttatcggt cactccatgg
     8401 gcggtaaagc ggtaatggca cttacggcac tggcccgcga tcgcatcgat aaactggtgg
     8461 cgatcgatat cgcaccggtc gactatcacg tacgccgtca tgatgagatt ttcgcggcta
     8521 tcaacgcggt cagtgaatcg gacgcacaaa ctcgccagca agcagcagca ataatgcgcc
     8581 agcatcttaa tgaagaaggg gtgattcagt ttctgttgaa atcttttgtt gacggggagt
     8641 ggcgctttaa cgtgccggta ttgtgggatc agtatccgca tattgtgggt tgggagaaaa
     8701 tcccggcatg ggatcaccct gccctgttta tccctagcgg caattctccg tatgttagcg
     8761 agcagtaccg tgatgattta ctggcacaat ttccacaggc acgggcgcat gtgattgcag
     8821 gcgcgggtca ctgggtccat gctgaaaaac cggatgcggt attacgcgct atccgtcgct
     8881 atctcaatga ttaacgcaac tgattaaaaa cccagcgtcc gcgtcgcttt ccgcgcgtgg
     8941 gcgttggcgt gactcatcgc gctgatgtat tatggcgcgc tatccatccg ggcagaggcc
     9001 tggctgattg tttcccccga agtcaccaag atcatggcca aagaacaaac ggaccgtacg
     9061 acattagatc tgttcgcgca cgagcgtcga ccgggacgac cgaaaactaa tccgctttcg
     9121 cgcgatgaac agctgcgtat taataaacgc aaccagctaa aacgcgacaa agtacgtggc
     9181 cttaagcgtg tcgaactgaa gctgaacgcg gaagctgtcg aggcgctgaa cgagctggcg
     9241 gagtcgcgca atatgagccg tagcgaactg atcgaagaga tgttaatgca gcaactggcg
     9301 gcgctgcgta gtcagggcat cgtttaaatt tccactttca tgtagcacag tgtgcagtcc
     9361 tgctcgtttg ctgattccga aagactgcct gttctgctat gattgccttt atccgtgggc
     9421 aattttccac ccccatttca ataagtttca agaggttatt tcactcatgg ctatcactgg
     9481 catctttttc ggcagcgaca ccggtaatac cgaaaatatc gcaaaaatga ttcaaaaaca
     9541 gcttggtaaa gacgttgccg atgtccatga cattgcaaaa agcagcaaag aagatctgga
     9601 agcttatgac attctgctgc tgggcatccc aacctggtat tatggcgaag cgcagtgtga
     9661 ctgggatgac ttcttcccga ctctcgaaga gattgatttc aacggcaaac tggttgcgct
     9721 gtttggttgt ggtgaccagg aagattacgc cgaatatttc tgcgacgcat tgggcaccat
     9781 ccgcgacatc attgaaccgc gcggtgcaac gatcgttggt cactggccaa ctgcgggcta
     9841 tcatttcgaa gcatcaaaag gtctggctga tgacgaccac tttgtcggtc tggctatcga
     9901 cgaagaccgt cagccggaac tgaccgctga acgtgtagaa aaatgggtta aacagatttc
     9961 tgaagagttg catctcgacg aaattctcaa tgcctgatgt gatgcggcgt agactcatgt
    10021 ctacgccgta ttaatagata atgccaatca aaataattgc tacaaatttg taacttttgc
    10081 tgttgtacct gtacaatgtc ccggtgttca agtggccttg ccgttgtaaa tgtaagctgt
    10141 gccacgtttt tattaacaat atttgccagg gacttgtggt tttcatttag gcgtggcaat
    10201 tctataatga tacgcattat ctcaagagca aattctgtca cttcttctaa tgaagtgaac
    10261 cgcttagtaa caggacagat tccgcatgac tgataacaat accgccctaa agaaagctgg
    10321 cctgaaagta acgcttcctc gtttaaaaat cctggaagtt cttcaggagc cggacaacca
    10381 tcacgtcagt gcggaagatt tatacaaacg tctgatcgat atgggtgaag aaattggtct
    10441 ggctacggta tatcgcgtac tgaaccagtt tgacgacgct ggtatcgtca cccgccacaa
    10501 ttttgaaggc ggtaaatccg tatttgaact gacacagcaa catcaccacg atcacctgat
    10561 ctgcctcgac tgcggcaagg ttatcgaatt tagtgatgat tccatcgaag cgcgtcagcg
    10621 tgaaattgcc gcaaaacatg gcattcgcct gactaaccac agtctctatc tttacggtca
    10681 ctgtgccgaa ggcgattgcc gcgaagatga acatgcgcac gaaggcaaat aagccagcct
    10741 gaatgagaaa aagccaacct gcgggttggc ttttttatgc aagggaaata agaaaagcga
    10801 gagttacagc tctcacttat ttgttattac tgcgaatttc ctgccaaact ttatcacaat
    10861 cggctttcac cgcctgatca tttccggttt gcgttgcacg cagacactgc tgataatcca
    10921 gtacgcggac actttcctgg tcggcaaaat gctgatgttg tttctctttc ttcagcacgt
    10981 ttaacacgct ctggcaggct tcaatttttt ctggcgaacc ttgtgcggtg ttgatacagg
    11041 cgctgtatgc ctctttcaga cgactgtctt cctgtggcgc ggttgattgc gcacaagcta
    11101 ccagccccga tgccattatg gcgatgagaa tcagtttttt catagcaaat tcctcaaacg
    11161 tgcggcaggc acaaaacctg ccgcgtcggg catcagaaga tggtgaatgg tgcgattacc
    11221 ataaatttca cgtcacgctc atcctggaag atgttgccgt aaccaccgcc ccagcttggg
    11281 atgtcggagt ggttgtcgta ttcggtgaag tgcagtttga acatcgtgcc tttggcgcga
    11341 ccgtcctgaa tggtgtagac cgcatccagg ctgtatgcag actcttcaat agtccggttt
    11401 ttgtcgtagt acgcatccgg attgctctgc caggtcgcag gtttagcatc ccatgcgtaa
    11461 acgtaggaag cgccgatggc gaagcctaga agattccagt ttttcaggtc atacatcgca
    11521 ccgaagaaga ccgctttttc gccgttagcg ttgaagtcgg aacggttatc ccaccagata
    11581 tccaggcggc cgttggagga agcgtaggct ggagtcatac gttgcaggaa gtatccctgc
    11641 tgaccgtcag ccttaaccca ggtgccttcg aggcgcaaat ccactacgtc agccgcccgg
    11701 taaccaaagg tcaacgcttg cagccaggcg gtgccgtcat aaaggtcgtt gacgctgcga
    11761 tcgtcaactt tatcgcgcgt accatagaac tggtagctgg tggttaacgg gctaccggcg
    11821 atatcaaatt tgtagctggc tttggcaaaa tattgatcga tatacccttc cgcctgacca
    11881 aatgccgctt ccagtacgaa gttatttttg aagtcgtatt tcgccccaag ggagtgcaga
    11941 taatcaactt tggtggtttt atcgttctga taaaactcat ccatttccag atgccacggc
    12001 gctttgtatt cgttggtcca catgtaggag aaactcaacg caccagcatc gccgtaatca
    12061 aaattcgccc cggcttccgc cccctgataa gtacctggca taaagctcca gtgcggcgct
    12121 aacagcgttt gaccagttgg ctgaatgtaa cctgccctcg cccaaaccgg accgtattta
    12181 aatttggccg cagctttata caggcttata ccgcttttgt cgccggacca gtcttcgtca
    12241 taggctttat tactttttga aaacgcgatt tcgttcgggt gggagctgtc gccgttttcc
    12301 gccatttcaa tcgccgtaaa cacagcaata tcaaggccga acatatcagc agcataaccg
    12361 gactgaaaat cgaggttggc attccaggtg gagtgagaaa ggttggtttt gtatttgtcg
    12421 ccgtcggtaa catctttacg gtcgcgttca cgctgccagt aatatatacc gccggttaag
    12481 gttgaatcgt cgatgaatcc ttcggcgcga gcctccggca ttgccataaa gcccgacatt
    12541 gctgtaacac cggcgatagc cagcgccagc gtactacgtt tgccactaaa cgtacgcatg
    12601 ggttaatcct ctttgacgta taaattgctg caccaaaggt gaacagcgaa aaaaataaaa
    12661 attaaaaaag tagcattgag aaaagtctcg ctgactacag gaatagacta tttttcgcga
    12721 cgcgaattat caatctcttt ctgtaaccag aatgtaagaa catgagcaac cgcacatatt
    12781 ttgatgattt ttgttacaag gcttaagttt gcggttgaaa ggcttgcaga agaataaaag
    12841 cgtaaatttc cagcaagatg aaagcggtta cattttattt aattatggtt tgtgatttta
    12901 aatttatttt atggataaat tgcgaagcgg aattaattcg cgaagaaaag cagaaaaaac
    12961 gccgcagagt gcggcgtttg ggattgtttg ctatttcttg ccggatgcga cgtaaatgcc
    13021 tcacccggcc tggtagaact taaaattact cgcctacttt cgcccaggta tcacgcagcc
    13081 caacggtgcg gttaaatacc ggtttttccg ctgtggaatg gcggctgtcg aggcagaagt
    13141 aaccttcacg ctcaaactgg aatgctttac ccgcaaccgc atctttcagc gacggttcag
    13201 caaagccctg tttgatcacc agcgattccg ggttaatcac cgacaggaaa tcatccgcag
    13261 cacctgggtt aggcacgctg aacagacggt cgtacagacg gatttcaacc ggcagcgcat
    13321 gtgccgcgct cacccagtga ataacacctt tgactttacg accatctgcc ggatctttgc
    13381 ttaaggtgtc ggcgtcataa gtacagaaga tggtggtgat attgccttcg gcatctttct
    13441 ccacgcgttc tgccttaatg acgtaagcat tacgcagacg cacttcttta cccagcacca
    13501 gacgtttgta ctgcttgtta gcttcttcgc ggaaatcggc gcgatcgatc caaatctcac
    13561 tgctaaacgg cacctggcgg ctacccattt ccggtttgtt cggatggttc ggcatggtaa
    13621 ccatttcgcc ttcgccctga tagttttcga taaccagttt caccggatcg ataaccgcca
    13681 ttgcgcgcgg ggcgttttcg ttgagatctt cacggatgca ggattccagc gacgccatct
    13741 caatggtgtt gtcctgcctg gtcacgccga tgcgtttgca gaactcacga atagaagcag
    13801 cagtgtaacc acgacgacgc agaccggaaa tggtcggcat acgcgggtca tcccagcctt
    13861 caacgtgctt gtcggtcacc agcaggttca gcttacgctt ggacatcaca gtgtattcca
    13921 gattcaggcg cgagaattcg tactggcgcg ggtgaacagg aatggtgatg ttgtccagca
    13981 cccagtcgta caggcgacgg ttgtcctgga actcaagcgt acacagagag tgcgtaatac
    14041 cttccagggc atcgctgatg cagtgggtga agtcgtacat cgggtagatg caccacttgt
    14101 tgccagtctg gtggtgttca gcaaatttaa tgcggtacag caccggatcg cgcatcacga
    14161 taaacggtga agccatgtcg attttcgcac gcaggcaggc tttaccttct tcaaaaccac
    14221 cggcacgcat tttttcgaac agcgccaggt tctcttcaac gctgcggtcg cggtacgggc
    14281 tgtttttacc cggttgcgtc agggtgccgc ggtattcgcg gatctgttcc ggcgtcagtt
    14341 catcaacgta cgccaggcct ttattgatca gttcgatcgc ataggcgtgg agctgatcaa
    14401 aataatcgga ggagtaacgg acgttaccag accagtgaaa acctaaccac tctacgtcgt
    14461 ttttgatcga ctcaacatac tcgatatctt cttttaccgg gttagtgtcg tcgaaacgca
    14521 ggttgcactg gcctttatag tcctgggcga tcccgaagtt caggcagata gatttcgcat
    14581 ggccaatatg cagatagcca ttcggctccg gcgggaaacg ggtgtgtact gtggtgtgct
    14641 taccactggc cagatcttca tcgatgatct gacggataaa gttagtcggg cgggcttctg
    14701 cctcactcat cgtggattcc tcaaagcgta aacaacgtat aacggcgtat gatcttataa
    14761 gcggaacagg ctgacaactg ttagtttttt aaaattgata atctaacaag gattatgggg
    14821 atgattgatg aaaaaaaaag cggcagagaa gatctccacc gcttgtcatt gttggatgcg
    14881 acgctcaagc gtcgcatcag gcataaagca gattactttt tgatttcata cagcggtgtt
    14941 tgacccgcca caacatggcc ctgagcttta atgatcaagc cactgaaatc gtcgatattg
    15001 ctgcaaacca ccgggctaat catcgagcgg gcgttagcgt tcaggtaatc cagatccatt
    15061 tccagaatcg gttgccctgc gcttacctgc gcaccctctt ccaccagacg tttaaagcct
    15121 ttaccttcca gcgctacggt gtcgataccc atatggacga cgatctccgc gcctttttcg
    15181 gtttccaggc agaacgcgtg gttggtgttg aagattttca cgattgtccc tgcggctggt
    15241 gatacgacga ttttatctgt cggtttcacc gccacaccgt cacccaccgc tttgctggcg
    15301 aatgcttcgt caggaacctg atccagtgcc acgacatcac cggtaatcgg cgataccagc
    15361 tccgcgatag ataccgcgtt tggtacagcc tgcggttttg ctacaggcgc ggcagttgac
    15421 ggagttgctt cagctgacgc agcggctacc ggaccacggg caacgacttt cttcatcgca
    15481 tcgccgatgg attctgcttt cgcgccaaca atcacctgaa tagtctgttt gttcagtttc
    15541 actacaccag aagcacccag acgtttacac atcgtatcgt tgacgcgggc agagtcagcc
    15601 acggtcaggc gcagacgggt gatacaggcg tcaattgctt tcaggttgtc agtgccgcca
    15661 accgcagcaa tatagttggt cgccagttga ttgaggcctt cttcagtgtt gctgttggct
    15721 tcttcagtaa cgatctcatc ttctttatct tcacgacccg gcgttttcag gttgaacatg
    15781 cggataacca aactgaacac cacgaagtag atagcgaaga agacaacacc catcaccagc
    15841 agcatccaga cgttctggct ggcggccggc aggttataca tcaacgcgta gtcgatagcc
    15901 cccgcagaga aagagaagcc agcatggata cccagcagtg ttgccacaaa caggctgata
    15961 ccggtcagca gtgcgtgcag gaggtacagc agcggtgcaa ggaacatgaa caggaattcc
    16021 agcggctcag tcacaccggt caggaacgca gtaacagcaa cagaaagcag catcccgcca
    16081 accatcggac gacgctcttt cggtgctgcg aagtacatcg ccagcgccgc acccggcaga
    16141 ccgaacatca taatcgggaa gaagccggac atgaacatcc ccgcggtgcc gtcaccggca
    16201 tagaagcggt tgatatcacc gtggaaaacc gtacccgccg cgttggtgaa ttcaccaatc
    16261 tggaaccagg cgatggtgtt cagcacctga tgcagaccgg ttgggatcag cagacggttg
    16321 atgaaaccaa agataccgga acccagcgcg cccgcagaaa cgatccactc gcctcctgca
    16381 tggatagcgt gctgtaccgg cggccagacg taaccaaaaa tggccgccag caccaggcag
    16441 aagaagccgg tggcaatcgg cacaaagcgt ttgccgccga agaagctcag gaagtccggc
    16501 agtttaatat cggaccaacg gttataggct gcgccaccaa ccagaccggt aatgataccc
    16561 gccagtacac ccatgtttat ttctgggttg atggtcacca tcgctttggt taacacaaag
    16621 taacctaccg cacccgccag cgccgccgca cctgcgctgt ctttcgacca gctggatgcc
    16681 acaccgatgg cgaagattaa tgcgaggtta tcaaaaatcg caccgcccgc ctgggcaata
    16741 aacgcaacgt taagtaaatc tggctgaccg aatcgcagca acagtgccgc caccggcagc
    16801 accgcgatag ggagctgtaa cgccctaccg agtcgctgga aaaaacctaa aatattcatc
    16861 ttattccccc tacgagaacc ctatttggct cgtttaaagc cgtattttta ttttgctgca
    16921 aattgtactg ccgatgttct gtaatcagat tgttagatca tctgctacag agtgtgtgaa
    16981 aatttaattc gtatcgcaaa ttaaacgcgt gtcttttgtg agttttgtca ccaaatatcg
    17041 ttatcatcac tcccttttac tggctaaacc agaaaactta ttttatcatt caaaaaatca
    17101 ggtcggattg acgcctgtct gcgcaaatcc aagttacgct taaagatgcc taatccgcca
    17161 acggcttaca ttttacttat tgaggtgaat aatgagactg atccccctga ctaccgctga
    17221 acaggtcggc aaatgggctg ctcgccatat cgtcaatcgt atcaatgcgt tcaaaccgac
    17281 tgccgatcgt ccgtttgttc tgggcctgcc aactggcggc acgccgatga ccacctataa
    17341 agcgttagtc gaaatgcata aagcaggcca ggtcagcttt aagcacgttg tcaccttcaa
    17401 catggacgaa tatgtcggtc tgccgaaaga gcatccggaa agctactaca gctttatgca
    17461 ccgtaatttc ttcgatcacg ttgatattcc agcagaaaac atcaaccttc tcaacggcaa
    17521 cgccccggat atcgacgccg aatgccgcca gtatgaagaa aaaatccgtt cttacggaaa
    17581 aattcatctg tttatgggcg gtgtaggtaa cgacggccat attgcattta acgaaccggc
    17641 gtcttctctg gcttctcgta ctcgtatcaa aaccctgact catgacactc gcgtcgcgaa
    17701 ctctcgtttc tttgataacg atgtcaatca ggtgccaaaa tatgccctga ctgtcggtgt
    17761 tggtacattg ctggatgccg aagaagtgat gattctggtg ctgggtagcc agaaagcact
    17821 ggcactgcag gctgcagttg aaggttgcgt gaaccacatg tggaccatca gctgtctgca
    17881 actgcatcca aaagcgatca tggtgtgcga tgaaccttcc accatggagc tgaaagttaa
    17941 gactttaaga tatttcaatg aattagaagc agaaaatatc aaaggtctgt aattgttatc
    18001 cctgccctgg ctccttgctc agggcaatat tttttaaaat cgggggtcag aatgtatgca
    18061 ttaacccagg gccggatctt taccggccac gaatttcttg atgaccacgc ggttgttatc
    18121 gctgatggcc tgattaaaag cgtctgtccg gtagcggaac tgccgccaga gatcgaacaa
    18181 cgttcactga acggggccat tctctccccc ggttttatcg atgtgcagtt aaacggctgc
    18241 ggcggcgtac agtttaacga caccgctgaa gcggtcagcg tggaaacgct ggaaatcatg
    18301 cagaaagcca atgagaaatc aggctgtact aactatctgc cgacgcttat caccaccagc
    18361 gatgagctga tgaaacaggg cgtgcgcgtt atgcgcgagt acctggcaag acatccgaat
    18421 caggcgttag gtctgcatct ggaaggtccg tggctgaatc tggtaaaaaa aggcacccat
    18481 aatccgaatt ttgtgcgtaa gcctgatgcc gcgctggtcg atttcctgtg tgaaaacgcc
    18541 gacgtcatta ccaaagtgac cctggcaccg gaaatggttc ctgcggaagt catcagcaaa
    18601 ctggcaaatg ccgggattgt ggtttctgcc ggtcactcca acgcgacgtt gaaagaagcg
    18661 aaagccggtt tccgcgcggg gattaccttt gccacccatc tgtacaacgc gatgccgtat
    18721 attaccggtc gtgaaccggg cctggcgggc gcgatcctcg acgaagctga catttattgc
    18781 ggtattatcg ctgatggcct gcatgttgat tacgccaaca ttcgtaacgc taaacgcctt
    18841 aaaggcgaca aactgtgtct ggttaccgac gccaccgcgc cagcaggtgc caacattgaa
    18901 cagttcattt ttgcgggtaa aacaatatac taccgtaacg gactttgtgt ggatgagaac
    18961 ggtacgttaa gcggttcatc cttaaccatg attgaaggcg tgcgtaatct ggtcgaacat
    19021 tgcggtatcg cactggatga agtgctgcgt atggcgacgc tctatccggc gcgtgcgatt
    19081 ggcgttgaga aacgtctcgg cacactcgcc gcaggtaaag tagccaacct gaccgcattc
    19141 acacctgatt ttaaaatcac caagaccatc gttaacggta acgaggtcgt aactcaataa
    19201 gagaaagtat gacaccaggc ggacaagctc agataggtaa tgttgatctc gtaaaacagc
    19261 ttaacagcgc ggcggtttat cgcctgattg accagtacgg gccaatctcg cggattcaga
    19321 ttgccgagca aagccagctt gcccccgcca gcgtaaccaa aattacgcgt cagcttatcg
    19381 aacgcgggct gatcaaagaa gttgatcagc aggcctccac cgggggccgc cgcgctatct
    19441 ccatcgtcac cgaaacccgc aatttccacg caatcggcgt acggcttggt cgtcacgacg
    19501 ccaccatcac cctgtttgat ctcagcagca aagtgctggc agaagaacat tacccgctgc
    19561 cggaacgtac ccagcaaacg ctggaacatg ccctgttgaa tgccattgct cagtttattg
    19621 atagctacca gcgcaaactg cgcgagctga tcgcgatttc ggtgatcctg ccagggcttg
    19681 ttgacccgga cagcggcaaa attcattaca tgccgcatat tcaggtagaa aactgggggc
    19741 tggtagaagc tctggaagaa cgttttaaag tgacctgttt cgttggtcac gatatccgta
    19801 gtctggcgct ggcggagcac tacttcggtg caagtcagga ttgcgaagac tccattctgg
    19861 tgcgtgtcca tcgcggaacc ggggccggga ttatctctaa cgggcgcatt tttattggcc
    19921 gcaacggtaa cgtcggtgaa attggccata ttcaggtcga accgctgggt gaacgctgcc
    19981 actgcggcaa ctttggctgc ctggaaacta tcgccgccaa cgctgccatt gaacaacggg
    20041 tgttgaatct gttaaagcag ggctaccaga gccgcgtgcc gctggacgac tgcaccatca
    20101 aaactatctg caaagccgcg aacaaaggcg atagtctggc gtcggaagta attgagtatg
    20161 tcggtcgtca tctgggtaaa accatcgcca ttgctatcaa cttatttaat ccgcaaaaaa
    20221 ttgttattgc cggtgaaatc accgaagccg ataaagtgct gctccctgct attgaaagct
    20281 gcattaatac ccaggcgctg aaggcgtttc gcactaatct gccggtggta cgttctgagc
    20341 tggatcaccg ctcggcaatc ggcgcttttg cgctggtaaa acgcgccatg ctcaacggta
    20401 ttttgctcca gcatttgctg gaaaattaat gtgcttttat agtggcgctt attgttgtca
    20461 atattctggg tagtccatga ccattaaaaa tgtaatttgc gatatcgatg gcgtgctgat
    20521 gcacgataac gtcgccgtac cgggtgcagc ggaatttttg cacgggatta tggataaagg
    20581 cctgccgctg gtgttgctga ccaactatcc ttcgcagact gggcaagatc tggcgaaccg
    20641 ctttgccacc gcaggtgtcg atgtacctga cagcgtgttt tatacctctg cgatggcgac
    20701 tgccgatttc ctgcgtcgcc aggaaggcaa gaaagcgtat gtggtgggcg aaggcgcact
    20761 gattcatgaa ctgtacaaag ccggtttcac tattaccgat gtgaaccctg atttcgtgat
    20821 tgttggcgaa acgagttcct acaactggga catgatgcat aaagcagcct atttcgtcgc
    20881 taacggtgca cgttttatcg ccaccaatcc ggacacccac gggcgcggtt tttatcccgc
    20941 gtgtggcgcg ttgtgtgcag gcatcgaaaa aatctccggg cgtaaaccgt tctatgttgg
    21001 taaaccgagt ccgtggatca tccgcgcggc attaaacaaa atgcaggcgc attcggaaga
    21061 aacggtgatt gtcggcgata acctgcgtac cgatattctg gcgggcttcc aggcaggtct
    21121 ggaaacgatt ctggtgcttt ctggtgtttc atcgctcgac gatatcgaca gtatgccgtt
    21181 ccgcccaagc tggatttacc cgtcggtcgc tgaaatcgac gttatctgaa tagcaaccac
    21241 gtctgcggac gtggttggct ctgatgacaa acgccaaact gcctgatgcg ctacgcttat
    21301 caggcctacg cagctcctgc aatatattga attttcatgc ttttgtaggc cggataaggc
    21361 gttcacgccg catccggcat gaacaaattg cactttgtca gcaatttggc ccccgtaaaa
    21421 tacaaacatt caataaaacc cgctgaaaat tatcgattca tcaataaata tatcctcaca
    21481 ccagaccttt tttcaccaat cgacaatcac cattacgttt tttatttttt caccgccaaa
    21541 tcgcttgcgc acagtgctgc tttgcggcat ttttttaaac aagcaaacac aacaagcaac
    21601 aaataccaga ttaacggaga aggttatgtg ttcaattttt ggcgtattcg atatcaaaac
    21661 agacgcagtt gagctgcgta agaaagcact cgagctgtca cgcctgatgc gtcatcgtgg
    21721 cccggactgg tccggtattt atgccagcga taacgccatt ctcgctcacg aacgtctgtc
    21781 aattgttgac gttaacgcgg gggcgcaacc tctctacaac caacaaaaaa cccacgtact
    21841 ggcggtaaac ggtgaaatct acaaccacca ggcattgcgc gctgaatatg gcgatcgtta
    21901 tcagttccag acagggtctg actgtgaagt gatcctcgcg ctgtatcagg aaaaagggcc
    21961 ggaatttctc gacgacttgc agggcatgtt tgcctttgcc ctgtacgaca gcgaaaaaga
    22021 tgcctacctg attggtcgcg accatctggg gatcatccca ctgtatatgg gctatgacga
    22081 acacggtcag ctgtatgtgg cctcagaaat gaaatcgctg gtgccagttt gccgcacgat
    22141 taaagagttc ccggcgggga gctatttgtg gagccaggac ggcgaaatcc gttcttatta
    22201 tcatcgcgac tggttcgact ttgatgcagt gaaagataac gtgaccgaca aaaacgagct
    22261 gcgtcaggct ctggaagatt ccgttaaaag ccatctgatg tctgatgtgc cttacggtgt
    22321 gctgctttct ggtggtctgg attcctcaat tatttccgct atcaccaaga aatacgcagc
    22381 ccgtcgcgtg gaagatcagg aacgctctga agcctggtgg ccgcagttac actcctttgc
    22441 tgtaggtctg ccgggttcac cggatcttaa agcagcccag gaagtggcaa accatctggg
    22501 cacggtgcat cacgaaattc acttcactgt acaggaaggt ctggatgcca tccgcgacgt
    22561 gatttaccac atcgaaactt atgatgtgac cactattcgc gcttcaacac cgatgtattt
    22621 aatgtcgcgt aagatcaagg cgatgggcat taaaatggtg ctgtccggtg aaggttctga
    22681 tgaagtgttt ggcggttatc tttacttcca taaagcgccc aacgctaaag aactgcatga
    22741 agagacggtg cgtaaactgc tggccctgca tatgtatgac tgcgcgcgcg ccaacaaagc
    22801 gatgtcagcc tggggcgtgg aagcacgcgt tccgttcctc gacaaaaaat tccttgatgt
    22861 ggcgatgcgc attaacccgc aggataaaat gtgcggtaac ggcaaaatgg aaaaacacat
    22921 cctgcgtgaa tgttttgagt catacctgcc cgcaagcgtg gcctggcggc agaaagagca
    22981 gttctccgat ggcgtcggtt acagttggat cgacaccctg aaagaagtgg cggcgcagca
    23041 ggtttctaat cagcaactgg aaactgcccg cttccgcttc ccgtacaaca cgccaacctc
    23101 aaaagaagcg tatctgtacc gggagatctt tgaagaactg ttcccgcttc cgagcgccgc
    23161 tgagtgcgtg cctggcggtc cttccgtcgc gtgttcttcc gctaaagcga tcgaatggga
    23221 tgaagcgttc aagaaaatgg acgatccatc tggtcgtgcg gttggtgttc accagtcggc
    23281 atataagtaa gaaatgcgaa acagccccgg tgaaatccgg ggctgtttca gttattgagg
    23341 tgcttgttga ttctgatatg cagtaaggaa atgttccggg tttgccacat cactgtttga
    23401 caactcatca ctgtaggcca gtatttcata gattaatttc ccaccactat tgttgaattc
    23461 cgaatttcat cctgtacgac aaaagaatgg ctgtagggtg gggaaaaaac acaaagtaaa
    23521 caataattga ctaatatagc gccacgctgt tcgcaaccta accaaatagt cactttcgag
    23581 caattttcct tgaaaaagag gttgacgctg caaggctcta tacgcataat gcgccccgca
    23641 acgccgataa ggtatcgcga aaaaaaagat ggctacgtag ctcagttggt tagagcacat
    23701 cactcataat gatggggtca caggttcgaa tcccgtcgta gccaccatct ttttggcggg
    23761 agtggcgaaa ttggtagacg caccagattt aggttctggc gccgcaaggt gtgcgagttc
    23821 aagtctcgcc tcccgcacca ttcaccagaa agtgttgtac ggatggggta tcgccaagcg
    23881 gtaaggcacc ggtttttgat accggcattc cctggttcga atccaggtac cccagccatc
    23941 ttcttcgagt aagcggttca ccgcccggtt attggggtat cgccaagcgg taaggcaccg
    24001 gtttttgata ccggcattcc ctggttcgaa tccaggtacc ccagccatag aagaaacaat
    24061 ctggctacgt agctcagttg gttagagcac atcactcata atgatggggt cacaggttcg
    24121 aatcccgtcg tagccaccaa attctgaatg tgtcgaatac gttcggcaaa ttcaaaaacc
    24181 aatttgttgg ggtatcgcca agcggtaagg caccggattc tgattccggc attccgaggt
    24241 tcgaaccctc gtaccccagc caatttattc aagacgctta catcgtaagt gcacccagtt
    24301 ggggtatcgc caagcggtaa ggcaccggat tctgattccg gcattccgag gttcgaatcc
    24361 tcgtacccca gccacattaa aaaagctcgc ttcggcgagc tttttgcttt tctgcgttca
    24421 ttcaatgtcg aatgcgatgt tgacacgtct tatccttcaa tgtcggatgc gacgctgccg
    24481 cgtcttatcc gacctacggt tggcacgcat ccggcaatgt tgtaaggcta caaccctaac
    24541 gcatatttca gcgcctgacg tttcaacacg ccagcacgct ctgccgccat taacccgaga
    24601 ttacgcacaa aacgcagcgg cggcagatta ttactgaatc cggcataaaa cagatccata
    24661 ccactttgca taatgaagtt atccgccatg cgccgcatct ggtaacgctt gaggacagga
    24721 taactggccc acgcttcacc gtaactgcgg gcattcacca gaacatcaat cagggcatcg
    24781 acatcacgat aaccaagatt caccccctgc cccgccagcg gatggatggt atgcgcggca
    24841 tcgcccacca gcgcaagccc cggctgcaca tactgcaacg catgtcggcg cgtcagcgga
    24901 aacgcaccag cggcaagcgg tgtaacgtaa cccagacgcg acgggaaatg cttcgcgatt
    24961 tccgcctgga gttgcgccat attcatattc tgcaactggc gaatacgcgc cggagagtca
    25021 taccacacca gcgatgccca gttatcaaac aacggcagaa acgcacgcgg tccgtccggg
    25081 gtaaattgct gccaggtgct gtcgccggga tcgttctcac actgtacgct aatcaacatg
    25141 cacgactgcg catactgcca ggcatgaacg ccaattcccg ccatctgccg cacctgagaa
    25201 tttgcgccgt cggcaccaat caccagtttc gcgcggatcg tttcaccgcc cttcagttcc
    25261 agctcctgaa gatcattatc tcgatgtaat gcaatcagcg agcctggcac acgtaacgtt
    25321 actttcggat gcgcttccag cgcctgccac agcgcctgtt gcagtacggt gttttccacc
    25381 atatagccaa gcaacggtag cttaagttca gcggcgtcaa acaccacatg cgccgtttcc
    25441 cactcccacg tttccagtct gcggtaagga tggcaacgca tagcctgtac tgcatcccag
    25501 acccctaacc ctttaagcaa tgataccgat gcggcgctga tcgccgagat ccgcacgtcc
    25561 ggttggctat cagcgacaaa cggtgcaggt tctgcgtgct cgatcaccgt taccgaaaat
    25621 ccgtgctgtg ccagccccag cgccagcgcg ccgccgacca ttcctccgcc gacaatggca
    25681 atttccgttg gttgatttgt catggtcaat catcctgtta gcaatatgct ttaagtttac
    25741 aggattttta cccccaaggg ctgataacgc tcatactggc cacaacggca gcaaagcatt
    25801 acactatgcg ccctgcattc ctggctacta tttcgcaaga gcaagtcgat gaccaaaaaa
    25861 ctccatatta aaacctgggg ctgtcagatg aacgagtacg attcatcgaa gatggccgat
    25921 ctgctggatg ccacccacgg ctatcaactg accgacgtgg cggaagaagc ggatgtgctg
    25981 ctgctgaaca cctgctcaat ccgcgagaag gctcaggaaa aagtcttcca tcagttgggt
    26041 cgctggaaac tgttaaaaga gaagaatcca gacctgatta tcggcgtcgg tggctgcgtg
    26101 gcatcgcaag aaggcgagca cattcgccag cgcgcccact atgtcgatat tatttttggg
    26161 ccgcaaacgc tgcaccgtct gccggagatg atcaactccg tgcgcggcga ccgcagcccg
    26221 gttgtagata tcagcttccc ggaaatcgag aagtttgacc gtctaccgga accgcgcgcc
    26281 gaagggccga ccgcgtttgt ctccatcatg gaaggctgca ataaatattg cacctactgc
    26341 gtggtgcctt acacccgtgg tgaagaggta agccgtccgt ctgacgatat tctgtttgag
    26401 attgcccagc ttgcggctca gggcgtgcgt gaagtcaacc tgctcggtca gaacgtgaac
    26461 gcctggcgtg gtgaaaacta cgacggctcc accggatcgt ttgccgatct gctgcgtctg
    26521 gttgctgcga tcgacgggat cgatcgtatt cgctttacca ccagccatcc gatcgaattc
    26581 accgacgata tcatcgaagt gtatcgcgac acgccggagc tggtgagctt cctgcatctg
    26641 ccggtacaga gcggttccga tcgcattctg aacctgatgg gccgtactca tacggcactg
    26701 gagtacaaag cgatcatccg taaactgcgt gcggcgcgtc cagatattca gatcagttct
    26761 gatttcatcg ttggcttccc tggcgaaacc accgaagact tcgagaaaac gatgaagctg
    26821 attgccgacg tcaatttcga catgagctac agctttatct tctctgcacg tccgggtaca
    26881 ccagccgccg atatggttga tgatgttccg gaagaagaga agaagcagcg tctgtatatt
    26941 ctgcaagaac gtatcaatca gcaagcgatg gcatggagcc gccggatgct cggcaccacc
    27001 cagcgtattc tggtagaagg gacttcgcgt aagagcatca tggagctttc cgggcgtacc
    27061 gaaaataacc gcgtggtcaa cttcgagggc acaccggata tgatcggtaa attcgtcgat
    27121 gtagaaatta ccgacgtcta cccgaactct ctgcgcggta aagtggtgcg tactgaagat
    27181 gaaatgggcc tgcgcgtggc agaaacaccg gaatcggtga ttgcccgtac ccgcaaagaa
    27241 aacgaccttg gcgtgggtta ttatcagccg taattctcag gcccgccgtt ccggtgggcc
    27301 ttgttttttc tctctttatc cccatcttta tcgcaatgct tgcgcctttt tcctgtggcg
    27361 tgaataattt ccttataggc cggtgaatgt tcagcttccg gcggcataca gataagagga
    27421 acggtttgaa catagacact cgcgaaatca ccctggagcc agcagacaac gcgcgtctgt
    27481 tgagcctgtg cggcccgttt gatgacaaca tcaagcagct cgaacgccgt ctcggcatcg
    27541 agatcaatcg ccgcgacaac cactttaaac tgaccggccg tccgatttgc gtcaccgctg
    27601 cggcagacat tctgcgtagc ctgtatgtcg atacagcccc gatgcgcggt cagattcagg
    27661 atatcgaacc ggaacagatc caccttacga ttaaagaagc gcgagtactg gagcaaagtg
    27721 cggagagcgt gccggagtac ggcaaagcgg tcaatatcaa aaccaaacgc ggcgtaatta
    27781 agccgcgcac gccaaaccag gcgcagtaca tcgccaatat tctcgaccat gacattacct
    27841 tcggcgttgg cccggcgggt acgggtaaaa cctacctggc agtggctgcg gcagttgatg
    27901 ccctggagcg tcaggagatt cgccgtattc tgctgactcg tccggcggtc gaagccggtg
    27961 agaaactggg cttcctgcct ggcgatttaa gccagaaagt agacccgtat ctgcgcccac
    28021 tgtacgacgc gctgtttgaa atgctgggct ttgagaaagt tgagaaactg attgagcgca
    28081 acgttattga agtcgcgccg ctggcctata tgcgtggtcg tacgctgaac gacgcgttta
    28141 tcattctcga tgagagccag aacaccacca tcgaacagat gaagatgttc ctgacccgta
    28201 tcggttttaa ctcaaaagcg gttatcaccg gcgacgtcac acagatcgac ctgccgcgta
    28261 atactaaatc aggcttacgt cacgccatcg aagtactggc cgatgtcgaa gagatcagct
    28321 ttaacttctt ccacagcgaa gacgtggttc gtcaccctgt ggtggcgcgt atcgttaacg
    28381 cctatgaagc ctgggaagaa gccgaacaaa aacgaaaagc ggcgctggcg gcagaacgca
    28441 agcgcgaaga acaggaacaa aaatgagtca ggtgatcctc gatttacaac tggcatgtga
    28501 agataattcc gggttaccgg aagagagcca gtttcaaaca tggctgaatg cggtgatccc
    28561 gcagtttcag gaagaatcgg aagtgactat tcgcgtggtc gataccgccg aaagccacag
    28621 tctgaatctg acctatcgcg gtaaggataa gccgaccaac gtgctctcct tcccgtttga
    28681 agtgccgcct ggcatggaaa tgtcgctact gggcgatctg gttatctgcc gtcaggtggt
    28741 tgagaaggaa gctcaggagc aaggcaaacc actggaggcg cactgggcgc atatggtggt
    28801 gcacggcagt ctgcatttgt taggttacga tcacatcgaa gatgacgaag cagaagaaat
    28861 ggaagccctc gaaacagaga ttatgcttgc tctgggctat gaggatccgt acattgccga
    28921 gaaagaataa tttgccaggc atttgactgg cagacgtccc cgccgttggt gattaacgac
    28981 ggcgttttga ttaactaaca tgagaaccca tacgacgcca tgagcgacga caattcacac
    29041 agtagtgaca cgataagcaa caagaaggga tttttctccc tgttactcag ccaacttttc
    29101 cacggtgaac cgaaaaaccg tgacgaactg ctggcgctga tccgtgattc cgggcagaac
    29161 gaccttatcg acgaagatac gcgcgatatg ctcgaagggg tgatggacat cgcagaccaa
    29221 cgcgttcgcg acatcatgat cccccgctcc cagatgatta ccctgaaacg caaccagacg
    29281 ctggacgaat gccttgatgt catcatcgag tccgcccact cacgtttccc ggtgattagc
    29341 gaagacaaag atcacattga agggattctg atggcgaaag acttgctgcc gtttatgcgc
    29401 agcgatgctg aagccttcag catggacaaa gtgttacgtc aggcggttgt cgttcctgaa
    29461 agtaagcgcg tagaccggat gctgaaagag tttcgctctc agcgttacca catggcgatc
    29521 gtcattgacg aattcggtgg ggtttccggt ctggtgacca ttgaagacat cctggaactg
    29581 attgttggtg agattgaaga cgaatatgac gaagaagatg atatcgactt ccgtcagctg
    29641 agtcgtcata cctggaccgt gcgcgcactg gcttccattg aagacttcaa cgaagcgttc
    29701 ggcacccact ttagcgatga agaagtcgac actatcggtg gtctggtgat gcaggcgttt
    29761 gggcatcttc cggcgcgtgg cgaaactatc gacatcgacg gttaccagtt caaagtggcg
    29821 atggccgaca gtcgacgtat tattcaggtt catgtcaaaa tcccggatga ctcaccccag
    29881 ccgaagctgg atgaataaaa ccgaaactgg atagataact acatggcttt tgcctcatta
    29941 attgaacgcc agcgcattcg cctgctgctg gcgttattat tcggtgcctg cggaacgctg
    30001 gccttctctc cttacgacgt ctggcctgcg gcgattattt cgttgatggg gcttcaggcg
    30061 ttgaccttta accgccgtcc actccagtct gccgctattg gcttttgctg gggatttggc
    30121 ctctttggca gcggtattaa ctgggtctat gtcagcatcg cgacctttgg cggaatgcct
    30181 ggcccggtta acatcttcct ggtggtgctg ctggcggcgt atttgtcgct gtataccgga
    30241 ctgtttgctg gcgtgctgtc gcgtctgtgg ccgaaaacca cctggctgcg cgtggcgatt
    30301 gccgcccctg ctctctggca agtgaccgag tttctgcgcg gttgggtact gacaggcttc
    30361 ccgtggttac agttcggcta tagccagatt gatggcccgt taaaagggct ggcaccgata
    30421 atgggcgtgg aagccattaa cttcctgctg atgatggtta gtggcctgct ggcactggcg
    30481 ttggtcaaac gcaactggcg tccgctggtg gtggccgtcg tgctgtttgc cctacccttc
    30541 ccgctgcgtt acatccaatg gtttaccccg caaccggaga aaaccattca ggtttcgatg
    30601 gttcagggcg atattccgca atcactgaaa tgggatggag atcagcttct taatacgctg
    30661 aagatttact acaacgcaac ggctccgctg atgggcaaat catcgttgat tatctggccg
    30721 gagtcggcga taaccgatct ggaaattaat cagcaaccgt tcctcaaagc actggacggt
    30781 gagttgcgcg ataaaggtag ctcgctggta accgggattg tcgacgcgcg tctcaataag
    30841 cagaaccgct acgataccta caacaccatc atcacactgg gtaaaggtgc accgtacagc
    30901 tacgaatcag ccgatcgcta taacaaaaac catctggtgc cgtttggcga gtttgtcccg
    30961 ctggagtcga ttctgcgtcc gttagcaccg ttctttgatc tgccgatgtc gtcgttcagc
    31021 cgtgggccat atatccagcc gccgctgtca gtaaatggta ttgagcttac tgcggctatt
    31081 tgctacgaga tcattctcgg cgagcaagtg cgcgataact tccgcccgga taccgactat
    31141 ctgctgacta tctccaacga tgcgtggttt ggtaaatcta ttggtccatg gcaacacttc
    31201 cagatggcgc gaatgcgtgc gctggagctg gcgcgcccac tgttgcgcag caccaacaac
    31261 ggcattacgg cggtgattgg cccgcagggt gagattcagg cgatgatccc gcagttcacc
    31321 cgcgaggtgt taaccactaa tgtgacaccg accaccggac tcacaccgta tgcacgtacc
    31381 ggcaactggc cactgtgggt gctgacggca ctgtttggtt ttgccgctgt gttgatgagt
    31441 ctgcgagcac gtaaacgttg atgtgcctga tgtatcctgc atgagcaaaa cgtactttct
    31501 cattgagctt tggggcatgg ttatcatgcc cctttttctt acggttcagg gatacaagcc
    31561 acattgccat attggggatg acgaataatt gcctgcggat caagcgagcg cgtcctgtcc
    31621 atttggatca acccgttctt cgccatgaca aatatttttc taccatccgc tgaa
//
LOCUS       DAZPEB010000025        31372 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZPEB010000025 DAZPEB010000000
VERSION     DAZPEB010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31372
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.025"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(15..3296)
                     /locus_tag="ACHURJ_001042"
     CDS             complement(15..3296)
                     /locus_tag="ACHURJ_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HFR1280079.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISCYKNVPVTTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSKRGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALMPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLLDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARV
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAIAFWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            3414..4607
                     /locus_tag="ACHURJ_001043"
     CDS             3414..4607
                     /locus_tag="ACHURJ_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFR1280080.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(4671..5924)
                     /gene="glyA"
                     /locus_tag="ACHURJ_001044"
     CDS             complement(4671..5924)
                     /gene="glyA"
                     /locus_tag="ACHURJ_001044"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFR1280081.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            6252..7442
                     /gene="hmpA"
                     /locus_tag="ACHURJ_001045"
     CDS             6252..7442
                     /gene="hmpA"
                     /locus_tag="ACHURJ_001045"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFR1280082.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISSGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIYYECFGP
                     HKVL"
     gene            complement(7487..7825)
                     /gene="glnB"
                     /locus_tag="ACHURJ_001046"
     CDS             complement(7487..7825)
                     /gene="glnB"
                     /locus_tag="ACHURJ_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFR1280083.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(7886..9220)
                     /gene="glrR"
                     /locus_tag="ACHURJ_001047"
     CDS             complement(7886..9220)
                     /gene="glrR"
                     /locus_tag="ACHURJ_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFR1280084.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(9210..9923)
                     /gene="qseG"
                     /locus_tag="ACHURJ_001048"
     CDS             complement(9210..9923)
                     /gene="qseG"
                     /locus_tag="ACHURJ_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFR1280085.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGCWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(10088..11515)
                     /gene="qseE"
                     /locus_tag="ACHURJ_001049"
     CDS             complement(10088..11515)
                     /gene="qseE"
                     /locus_tag="ACHURJ_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFR1280086.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGVVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(12073..15960)
                     /gene="purL"
                     /locus_tag="ACHURJ_001050"
                     /gene_synonym="purI"
     CDS             complement(12073..15960)
                     /gene="purL"
                     /locus_tag="ACHURJ_001050"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFR1280087.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRILGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRILHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            16221..17777
                     /gene="mltF"
                     /locus_tag="ACHURJ_001051"
     CDS             16221..17777
                     /gene="mltF"
                     /locus_tag="ACHURJ_001051"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFR1280088.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLCNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(17774..18277)
                     /gene="tadA"
                     /locus_tag="ACHURJ_001052"
     CDS             complement(17774..18277)
                     /gene="tadA"
                     /locus_tag="ACHURJ_001052"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFR1280089.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(18335..18970)
                     /gene="yfhb"
                     /locus_tag="ACHURJ_001053"
     CDS             complement(18335..18970)
                     /gene="yfhb"
                     /locus_tag="ACHURJ_001053"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFR1280090.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDKPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            19179..20027
                     /locus_tag="ACHURJ_001054"
     CDS             19179..20027
                     /locus_tag="ACHURJ_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFR1280091.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            20083..20343
                     /gene="yfhL"
                     /locus_tag="ACHURJ_001055"
     CDS             20083..20343
                     /gene="yfhL"
                     /locus_tag="ACHURJ_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFR1280092.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            complement(20537..20617)
                     /gene="shoB"
                     /locus_tag="ACHURJ_001056"
     CDS             complement(20537..20617)
                     /gene="shoB"
                     /locus_tag="ACHURJ_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFR1280093.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(21037..21417)
                     /gene="acpS"
                     /locus_tag="ACHURJ_001057"
     CDS             complement(21037..21417)
                     /gene="acpS"
                     /locus_tag="ACHURJ_001057"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFR1280094.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            complement(21417..22148)
                     /gene="pdxJ"
                     /locus_tag="ACHURJ_001058"
     CDS             complement(21417..22148)
                     /gene="pdxJ"
                     /locus_tag="ACHURJ_001058"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFR1280095.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKMRDACKRLTDAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(22160..22888)
                     /gene="recO"
                     /locus_tag="ACHURJ_001059"
     CDS             complement(22160..22888)
                     /gene="recO"
                     /locus_tag="ACHURJ_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFR1280096.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTYCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(22900..23805)
                     /gene="era"
                     /locus_tag="ACHURJ_001060"
     CDS             complement(22900..23805)
                     /gene="era"
                     /locus_tag="ACHURJ_001060"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFR1280097.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(23802..24482)
                     /gene="rnc"
                     /locus_tag="ACHURJ_001061"
     CDS             complement(23802..24482)
                     /gene="rnc"
                     /locus_tag="ACHURJ_001061"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFR1280098.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(24755..25729)
                     /gene="lepB"
                     /locus_tag="ACHURJ_001062"
     CDS             complement(24755..25729)
                     /gene="lepB"
                     /locus_tag="ACHURJ_001062"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFR1280099.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(25745..27544)
                     /gene="lepA"
                     /locus_tag="ACHURJ_001063"
     CDS             complement(25745..27544)
                     /gene="lepA"
                     /locus_tag="ACHURJ_001063"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFR1280100.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(27742..28221)
                     /gene="rseC"
                     /locus_tag="ACHURJ_001064"
     CDS             complement(27742..28221)
                     /gene="rseC"
                     /locus_tag="ACHURJ_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFR1280101.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(28218..29174)
                     /gene="rseB"
                     /locus_tag="ACHURJ_001065"
     CDS             complement(28218..29174)
                     /gene="rseB"
                     /locus_tag="ACHURJ_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFR1280102.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGDEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(29174..29824)
                     /gene="rseA"
                     /locus_tag="ACHURJ_001066"
     CDS             complement(29174..29824)
                     /gene="rseA"
                     /locus_tag="ACHURJ_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFR1280103.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(29857..30432)
                     /gene="rpoE"
                     /locus_tag="ACHURJ_001067"
     CDS             complement(29857..30432)
                     /gene="rpoE"
                     /locus_tag="ACHURJ_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFR1280104.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(30429..30584)
                     /gene="rseD"
                     /locus_tag="ACHURJ_001068"
     CDS             complement(30429..30584)
                     /gene="rseD"
                     /locus_tag="ACHURJ_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFR1280105.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            30840..>31372
                     /locus_tag="ACHURJ_001069"
     CDS             30840..>31372
                     /locus_tag="ACHURJ_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR1280106.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRR"
BASE COUNT         7502 a         8403 c         7920 g         7547 t
ORIGIN      
        1 acagttaata gggtttagtg aacgtagtta aaaataaaag gcatcacagt ggtgaataat
       61 gccgctttcg gccaggccgg attaatcgcc gtcaattcag cgcgagcaga ctcatattgc
      121 gcaacctccc cgagtcccgc ggacgccagc atcgccacca tcaggcattt ttctttatgc
      181 tgctgttgta aatcgccata cagcgataac aggtcaggtt gtgaaacagc aaagaaatcc
      241 gcctcaatac tggttttcgc catctcttgc gcccactgtt tcatttcact aaacagttgc
      301 tgtgcggttt gctgttcacc cagcagtcgc agcgccattc cttgccagaa gagataatca
      361 accggctgat cgttgtaata actgtggatg ttaatggtgc gatcgccggt cgccgccaga
      421 cgtaaacaac gcatcgcttc agtttcatcg ccctgcgcgt tggcgcatat cgcctgccag
      481 aaccagatgt cgttatcagt ttgccccggt aaacggcctt cgcttaaatt ctccggataa
      541 tgcagcgcgg catgaagcag ttcgctggcc tgctgcggct ctctggcatc aagatgctgc
      601 caggcgcgta ataactggtt gaggataaac tgactggtga ccttcccttc cccgccttcc
      661 cacgggtgga atttacgcgt ggcgagaatg tccgccgctt tgtctgcctg acccgtgaga
      721 tgccacaaat tgagcagttc tgcggtcatg tcgtcgcgtt tcagcgcaat ttccagatta
      781 ttttccagac gcgccagtcg tttctccggt gtggctccac ttaacttatc aagcaaatcc
      841 cgttcgaaaa gcagacgtgc atcctgcggc gcaagctgat aagcattatc aagataacgc
      901 gcggccagct cataatcgtg ttgcttattc cacgcatgga tcgctaaccc gcgccagccg
      961 tcggcaaact ccggcgacat ctctacgcaa cgttgccaaa aggcaatggc tttgttgtag
     1021 ctacgtttgt tgtagtagaa gcaggccagt aaatggcgag caaaccagca ctcttcaata
     1081 ctctccagcg ccgccacttc ttccagcgta ttcgggaaac ggacaaactg cgggaagaca
     1141 tcaatggctt ttgcgaccag ttcgccacgt tcggctttcg gcagcaagct ggcttgcagg
     1201 taaagcggca gcgtgcgctg gcagtccagt gcgttcagca tctctgccgc cagggtgggc
     1261 attccccagt taatcagttg cccggcggtc atcagggcgt taacgtcgcg tccctgacac
     1321 agaccgcgcc actgcgcgag agctgattcg ctacgaccat cgaaccagtt cagccaccac
     1381 agagtggcgt tcagcggata atcgcgcagc agtttctcgc gctgcacacg cgcgttgtcc
     1441 tgacgaccac ttaacaccag cagcagatta tgcaggcaaa gcacttcctg attggttggg
     1501 caggcgcgaa gactttgttg gcaaaaatcc agaccagcgt cgaagttacc attacgcgcc
     1561 gccagtcgtg ccaggccata atagccaccg gctttgctgt tgccgctcca gaccgcacgc
     1621 cagaaatcct cttcggcttg ttgatattgt ccctgacgtt cgtaagcact ggcgcgaatc
     1681 aaactcgcct gtccgcactg cggatttttg ttcagcgcat gtgcgcgttt cagagcctga
     1741 gtggcatacg ccaccgcttg cgggaaatcg gcgcggttat attccagcat cgccagcgcc
     1801 aggttacagc ggtaatccag cggatccagc gccacgccgc gcaggtagta atcgaacggt
     1861 gaacggctgg cgtgatgata ttgctccaga tgctgaccga taaaccaggc ttcatctgta
     1921 ctggtaatgt cttgtgctgc cagtggcgct ttggcgacgt ccagcaacgg caactcttgc
     1981 ggctgatgtt cctgataact cagtacaata ttgccgtcgg catcggagag ctcaatggtc
     2041 agcctttcag gattgatacc gtgcaacacg ccctggatgg cggtcgcagg catcagtgcc
     2101 acggcatcat caagtaacgc gttgcatttg ccgatttcgc ggatcgccag gcggtatccg
     2161 ttcaacggag agatggcata cagcccccac tcaatccccc gcttactacg ctggagtttt
     2221 atcaccgcat cgcgggaggc attttgcacc atgcccaaag aatgataagg caggaaatac
     2281 tgctcaaaac gcttctcttc gtaagcatca agccaggtaa aatcgggctg gttatcggca
     2341 aaaataccgg tcatcagttc gatatacggg ccgttattgt cggtcagact cttatcccac
     2401 gcctggccaa attcactgtg tccccaactc cactgttttt tacctggcgc aatatggtgg
     2461 ttggcaacgt gcagcaaacc gccatcttca tcgtgacacc acgcgccgac aaaatcgtac
     2521 tgcgattttt cggccatata tgaggttgta acaggcacat ttttatagca agaaatgtcc
     2581 actccggcgg agtagtccac tttgtagtaa gtgccggtgg cgatggggaa agcggagacg
     2641 gcccgtttgc cgtgatcaaa caccgcagtc acatccggtg ggaaaacgct ctgatgccct
     2701 tccccccctt tcactgccgg gttggcccac cacaagaaat gacgcggcgt ggcgttaccg
     2761 ttatagacgc ggctggcgat ttccagcgcc gcccggtcag ggcgcagggt gaaacctgtc
     2821 atcacctgta aaccatgcat cggctccgtt tcgcctaccc acaccgtctg tgcaccgtct
     2881 tcatgggctt cgagggtgaa atcaacgggc ataaaggtgg tcgggcgatg atgttgcggc
     2941 cagttaaact caatcccacc ggaaatccac ggccccagca gccccaccag cgcaggttta
     3001 atgacttcat tgtgataaac aaaatcgcgc tgtttcactt tatcccacgc gcgatgcacc
     3061 cgaccgccca gttccggcag gatcatcact ttgatgtagt cgttttccag ccacaccgcc
     3121 tgccaggatt tcagggtttt ctgctcgctc agcgtatcgg tcacgccgta gggataaacc
     3181 gcgccggacg atccctgata aacgcgattt tccaggaaca tgggatgtat atcctgcggc
     3241 ccggtttcat aggtcgggat ctcaacgcgc tcttgccaca cttttactgg agtcatggtg
     3301 ccctcaatat taacaagaca tactgaatta aaagatttgt ggcagtgtat tgaacaatct
     3361 ggcaatgttt tcgcggaata atcacgcaat taactaaaca aggtttagtg aagatgagag
     3421 cctgcattaa caatcaacag attcgccacc ataacaaatg cgtgattctg gaactgctgt
     3481 accggcaaaa gcgcgccaat aaatcaacgc tggcccggct ggcgcaaatt tcgattccgg
     3541 cagtcagtaa tattttgcag gaactggaaa gcgaaaaacg ggtggtgaat attgacgatg
     3601 aaagccagac gcgcgggcat agtagcggta catggctgat tgcgccggaa ggtgactgga
     3661 cgctgtgcct gaacgtgacg cccaccagta ttgagtgtca ggttgctaat gcttgtttaa
     3721 gtccgaaagg tgaatttgag tatttacaga ttgatgcacc gacgccgcag gcgctgctgt
     3781 ccgaaatcga aaaatgctgg catcgccacc gtaaattgtg gccggaccgc accatcaatc
     3841 tggcgctggc aatccacggt caggttgatc cggtgaccgg cgtgtcgcaa accatgccgc
     3901 aagcgccgtg gacaacgccg gttgaggtga agtatctgct ggaagagaag ctgggcattc
     3961 gggtgatggt cgataatgac tgcgtgatgc tggcgctggc ggagaaatgg caaaataatt
     4021 cgcaggaacg ggatttctgc gtgatcaacg ttgattacgg cattggctcg tcgttcgtga
     4081 ttaacgagca aatttatcgc ggcagtttgt atggtagcgg acaaattggt cacaccatcg
     4141 ttaatccgga tggcgtcgtc tgcgactgtg gacgttacgg ctgcctggaa accgtcgcct
     4201 cgttaagtgc attaaaaaaa caggcgcggg tatggctaaa atcacaaccg gttaatactc
     4261 agcttgatcc tgaaaaactg acaaccgcgc agttaatcgc tgcctggcag agtggagaac
     4321 cgtggatcac cagctgggtt gatcgctctg ccaatgccat tggtttgagt ctgtataact
     4381 tcctcaacat cctcaatatt aatcagattt ggttgtacgg tcgcagttgt gcctttggtg
     4441 agaactggct taatactatt attcgccaga caggatttaa cccgttcgac cgcgacgaag
     4501 gaccgagcgt gaaagcgacg caaattggct tcgggcaatt aagccgcgca caacaggtgc
     4561 tgggaattgg ctatttgtat gttgaggcgc agttacgaca gatttgatgg cgcgataacg
     4621 tagaaaggct tcccgaagga agcctttttt caacagatta accgtttcgc ttatgcgtaa
     4681 accgggtaac gtgcgcagat gtcgagaact ttacctttga tgcgctcgat aacggcttca
     4741 tcattgatgc tgtccagcac gtcacacatc cagccagcca gttctttcgc ttctgcttct
     4801 ttgaagccgc gacgcgtaat tgccggagta cccacgcgaa taccggaggt cacaaacggg
     4861 ctcttcggat cgttcggtac gctgtttttg ttgacggtga tgttagcacg gcccagagcg
     4921 gcgtctgctt ctttaccggt caggttttta tcaaccagat caaccaggaa caggtggtta
     4981 tcagtgccgc cggaaaccac tttgtagccg cgctcgagga acacttctac catcgcttta
     5041 gcgtttttcg cgacctgctg ctggtaagtt ttgaactcag gctccatcgc ttctttcaga
     5101 gcaaccgctt taccggcgat tacgtgcatc aacggaccgc cctgaccacc agggaaaacg
     5161 gcagagttca gttttttgta cagctcttcg ctaccacctt tcgccaggat caggccgccg
     5221 cgcggacccg ccagggtttt gtgagtggtg gtagtaacaa cgtgagcatg aggaaccggg
     5281 ttcgggtaga cgccagcagc aaccaggccc gcaacgtgcg ccatatcaac gaacaggtaa
     5341 gcaccgatgc tgtcagcgat ttcacgcatt ttcgcccagt ccaccacgcc ggaatatgca
     5401 gagaaaccac cgataatcat tttcggcttg tgttctttgg cttgtttttc cagatcggcg
     5461 tagtcgatat gaccggtagc atcgataccg taaggaacga tgttgtacag tttaccggag
     5521 aagttaaccg gagaaccgtg agtcaggtga ccgccatgcg ccaggttcat acccagaacg
     5581 gtatcacctg gttccagcag cgcggtgtag accgcaaagt tagcctggga gccggagtgc
     5641 ggctggacgt tagcgtagtc agcgccgaac agttctttcg cacgatcgat cgccagttgt
     5701 tcaacgatat caacatactc gcaaccgccg tagtagcgtt tgcccggata accttcagca
     5761 tatttgttgg tcagctgaga accctgcgcc tgcattacgc gcgggctggt gtagttttcg
     5821 gaggcgatca gttcgatgtg ctcttcctga cgtacttttt cctgctccat agcctgccac
     5881 agttcggcat cataatcggc aatgttcatt tcacgcttta acatccgcat ctcctgactc
     5941 agctaacaat aaaatttttg gcctttatag gcggtcctgt tggacaacgg cgaacagtat
     6001 aaccgaatca ttgtgcgata acaggtcttg acaaaggaat ttacgcaaac gattaccttc
     6061 aggctacgca aggctttgga gaataaagag cttgcaaccg gaaacggatt tcttttcagg
     6121 tttgtgatgc aaatttttca cttcatcaca ttctttctga aaaacaccaa agaaccattt
     6181 acattgcagg gctatttttt ataagatgca tttgaaatac atcaattaag atgcaaaaaa
     6241 aggaagacca tatgcttgac gctcaaacca tcgctacagt aaaagccacc atccctttac
     6301 tggtggaaac ggggccaaag ttaaccgccc atttctacga ccgtatgttt actcataacc
     6361 cagaactcaa agaaattttt aacatgagta accagcgtaa tggcgatcaa cgtgaagccc
     6421 tgtttaacgc tattgccgcc tacgccagta atattgaaaa cctgcctgcg ctgctgccag
     6481 cggtagaaaa aatcgcgcag aagcacacca gcttccagat caaaccggaa cagtacaaca
     6541 tcgtcggtga acacctgttg gcaacgctgg acgaaatgtt cagcccgggg caggaagtgc
     6601 tggacgcgtg gggtaaagcc tatggtgtac tggctaatgt atttatcaat cgcgaggcgg
     6661 aaatctataa cgaaaacgcc agcaaagccg gtggttggga aggtactcgc gatttccgca
     6721 ttgtggctaa aacaccgcgc agcgcgctta tcaccagctt cgaactggag ccggtcgacg
     6781 gtggcgcagt ggcagaatac cgtccggggc aatatctcgg cgtctggctg aagccggaag
     6841 gtttcccgca tcaggaaatt cgtcagtact ctttgactcg caaaccggat ggcaaaggct
     6901 atcgtattgc ggtgaaacgc gaagagggtg ggcaggtatc caactggttg cacaatcacg
     6961 ccaatgttgg cgatgtcgtg aaactggtcg ctccggcagg tgatttcttt atggctgtcg
     7021 cagatgacac accagtgacg ttaatctctt ccggtgttgg tcaaacgcca atgctggcaa
     7081 tgctcgacac gctggcaaaa gcaggccaca cagcacaagt gaactggttc catgcggcag
     7141 aaaatggcga tgttcacgcc tttgccgatg aagttaagga actggggcag tcactgccgc
     7201 gctttaccgc gcacacctgg tatcgtcagc cgagcgaagc cgatcgcgct aaaggtcagt
     7261 ttgatagcga aggtctgatg gatttgagca aactggaagg tgcgttcagc gatccgacaa
     7321 tgcagttcta tctctgcggc ccggttggct tcatgcagtt taccgcgaaa cagttagtgg
     7381 atctgggcgt gaagcaggaa aacatttatt acgaatgctt tggcccgcat aaggtgctgt
     7441 aatttgatgt tgccggatgg aaacatccgg caacccttga cgcggtttaa attgccgcgt
     7501 cgtcctcttc accggtacgg atgcgaatga cccgtgccac gtcaaagacg aagattttac
     7561 cgtcaccgat tttgccggtt tgcgccgtgc gaataatggt atcgacacag gtatcgacaa
     7621 tgtcgtccgg tacgacaatc tcaattttca ctttcggcag aaaatccacc atatactccg
     7681 cgccgcggta cagctcggta tggcctttct ggcgaccaaa gcctttcact tcggtcaccg
     7741 tcatgccggt aataccgact tcggccagtg cttcgcggac atcgtccagc ttgaagggtt
     7801 ttataatcgc atcaatcttt ttcatgctat tccttgaaaa ggtcgcctgt cttttgatct
     7861 gctaaacgta acacataacg ccaattcatt ccttgaaatc gtttgcatcc agctcgtgtc
     7921 gggaaagcag tttataaaat tctgtccggt tgcgccccgc cattctcgcc gcgtgggtga
     7981 cgttgccttt ggtaatttgc agcagcttac gcaaatagtt gagttcaaac tgattacgtg
     8041 cctcaacaaa ggttggcagc gccgtgtttt caccctccag cgcctgctct actaatgcat
     8101 cgctaataac cggagatgag gtcagcgcca cgcactgttc aatcacgttg accaactggc
     8161 gcacattacc cggccagcta gcggtcatca ggcgtttcat cgcatcagtg gagaacgcgc
     8221 ggacaaacgg tttatgtcgc tctgccgcct ggcgcaacag gtgatttgcc agaagcggaa
     8281 tgtcttctgt gcgctctgcc agagccggaa ttttcaggct gacaacgttg aggcggtaat
     8341 agagatcttc gcggaattcc ccgcgcgcca tcgcttttgg cagatcacgg tgagtggcag
     8401 aaataatccg cacattgata tcaatatcgc ggttactgcc cagcgggcgc actttacgct
     8461 cctgcaacac gcgcagcagt ttgacctgta acggcgcagg catatcgcca atttcatcga
     8521 gaaatagcgt tccgccttcc gccgcctgga ataaaccttc gcgattgctg acagcgccag
     8581 taaacgcgcc acgcgcatga ccaaacagct ccgactccag caattgctcg ggcaatgcgc
     8641 cacagttaat agcaataaat ggtttgctgt tgcgcgggct ggcgttgtgg atagcctggg
     8701 cgaaaatctc tttcccggtg ccgctctgac cgttaatcaa aacgctgacg tctgattgcg
     8761 ccaccagccg cgcctgttcc agcaaacgca gcatcagcgg gctgcgggtg acaattgcct
     8821 cgcgccagcg ttcatcggtg gctggcgcgg attgctccag cgcatcgtca attgcctgat
     8881 atagcgcgtc tttgtcgaca ggcttggtga ggaaactaaa aacgccctgc tgtgttgcag
     8941 caacggcatc ggggatagaa ccatgcgcag taagaataat tactggcatt cccggctgca
     9001 ctttctggat ttcagcaaat agctgcatac cgtccatttc atccatccgc aggtcgctga
     9061 taactaaatc tactttttcg cgattcagta cccgtaatcc ttcagcgcca ctttccgccg
     9121 tgaccacact gtagccttcg ctggtcaggc gcaggccaag cagtttcagc aatcccggat
     9181 cgtcatcgac caataataaa tgcgcaggtt tatggctcat caggagtgac ctcatgggtg
     9241 gatggcgcgg gcttttcact ctcgtgcggc gtatccggcg agaaatttcc agccggtttg
     9301 cgggtcgaga gctggcgttc aatatcggtc aggttttcca gcttgcgggt ggtgagttcc
     9361 agttgctgct gtagaacgtg atgttgctgg cgcaatgtat ccagctcgct gtcgctggac
     9421 tgctggagtt tactgtaacg ttggcgctct tccgccagtt gcagttgcag cgcctgaccg
     9481 tcgcgccaga gttgatacag tgggcgaacc tgtgccggga tctcggtact taacgcttca
     9541 atacgcgcga ccagctggcg gcgctcatac ggcgtaattt tggcgtcggc gagcaaaatc
     9601 ccttgtttaa aggtattttg ccagcagccg tcgtcatatt ggcgagcttg ctgacgcgac
     9661 tgcgcaggca ttaaacgatc agcacaatcc atcgcccgca gccagtaaag cggattggtt
     9721 tcggttgatt taccttgcag cgcccagatg tcgctacatt cagtagaaag atagtcagcc
     9781 agttgataaa ccggaatttt ttcttctgct ggcgtatcaa tggctggctt attgtgattc
     9841 tgcacgcaac ccagcaatgc cagacatggc agccctgcca gccacagccg tcggggcaat
     9901 aatcgttgaa aaatgtgtcg catattcacc agacttaaag cctatcccag tgggcgtaat
     9961 tgttgcagac agtctggaca tggacagcgc ggagaaaccg gagcgtacat atcgtacgtg
    10021 aagatttcga gcactgcccg gagccgaact gacaaataaa atagcctgat aggataggct
    10081 gttagcatta tttcgtgttt ttcgacgacg gtaattcaat gcggaaacaa acgtcttgcc
    10141 cgctctcgtc gaccagatac agttcccctt gcatacggcg aatacaatcc ctggcaatgc
    10201 ttaatcccag accgctgccc ttcaccaccc cttttcgctg gtggcttccc tgaaaaaagg
    10261 gttcgaagat catggcgcgt tcctcttgcg gaatgggcgt gcctgtattg atgacatcaa
    10321 tataaacccg cgcaccatgt gaactgctgc gaaggcaaat gttaccggat tcagccccgt
    10381 agtgcaccgc attggagtaa agattatcca gtacgctcat cagcagcatt ggctccgcca
    10441 ggcaagctgt tgctttgaga tcgacgtcgg tatgcatcat tttagcccgt gcgggcaggc
    10501 tatgagcaga aaccactgtc tccaccagcg gtgctaattc aacattctcc agttccaccg
    10561 cactgtccgc ctgtttacgg ttgtaatcaa gcagttgttc gatcagtttt tgcaaattgc
    10621 ggctgctgct atcaagaatg ctcaccacct ctttttgctc tggcgtaagc ggcccgacaa
    10681 cctggtcagc cagtaattca gtgccctcgc gcatactcgc cagtggcgtt tttaattcat
    10741 gagataaatg tcttaaaaat tgatggcgtt gggattccag ccatgacagg cgctcactta
    10801 accaaagaat acgttgccca accgagcgta actcgctcgg tccactgaac gaaacgctat
    10861 tgcccagaga acgcccttcc cccagacggt tgatcatgcg ctcgatattt ttcaccggcc
    10921 cgataatcat ccgcgtgaaa agtagcacca tcaccagact caccagaaat agcaccagcg
    10981 attgccaacc aaaatattgc ccacgttcgg cgatttcacg ctgaagttgc tgcccacgag
    11041 agaacaccac tgtgcgcgtg gcctgcacca tttcggtatt ggcactggca aaggcttcaa
    11101 gacgcgcggc ggcggcagca tcgggaccac tgttgttaca ctgaagttgg gccagattgt
    11161 tcaaatcctg acgcaatgcc tggtaaagtt tatcgtctgg cagtacgcct gcgtgggcat
    11221 cgagcatttc gctgtaacgc ttgcgctggc tttgataaac cttcgcaagc gtagggtcgt
    11281 ccagcacgca atactgacgg tagctacgct ccatctccag cgccgcgttg gtcatcgctt
    11341 cactacgccg ggcatcaata agcgtagtgc ggttaaccag cgccgcctga tcgctaagcg
    11401 cattcaggct ttgccaggct tgccatgcca gcaccaacag gggtagtaga attagcaaaa
    11461 atgccagcat taccagttgt cgtaatgagc ggggaaaaac gggccagcgt ttcaaggtgt
    11521 tactctcgtc agacgcgaat agcctgatgc taaccgaggg gaagttcaga tacaacaaag
    11581 ccgggaatta cccggctttg ttatggaata aggcggtgcc taactcgacg tttcgcccga
    11641 tggttgatat agctacgctg atatcagaag ttggacggca ggcaccttgt tgtgcgtcat
    11701 tcgaatttta tgtagcacgt cccgaagggg ctgacataag tcggtgaatg agccactgct
    11761 tactattatg cagtaactgt gccaataaag aaaatagttt ggtaacgtac tgattatacg
    11821 ttgctttgag ggatttatgg cttcttcgct atttgaatgt aaatcagcca ctgtgtcgct
    11881 aaaaagagac agcttaagat aaacttatta gattacaata aaatcaatca cttaagtgtc
    11941 gccagaaagc gacacggtaa accacccatt gtcgtgattt acagacacaa aaaagctccc
    12001 ggagttggga gcttatgata gtggttggtg cttaatgccg catccgggct gcaaaaccaa
    12061 tgaactgatg aattacccca actgcttacg cgcattgcgg aaaatgcgca tccatgggcc
    12121 atcctcgccc cagttttccg gatgccagga gttgctgaca gtacggaaaa cacgttccgg
    12181 gtgcggcatc ataatggtga ctcgaccact ttcagtcgtg actgccgtaa taccgttcgg
    12241 tgaaccgttc gggttagccg ggtaggtttc agtgactttg ccgaagttat cgacatagcg
    12301 cagtgccacc agccctttgc tttccagtgc cgccagatgc gccgcatcac gcacttccac
    12361 gcgcccttca ccatgagaga cagcaatcgg catttgcgag cccaccatcc cctgcaacaa
    12421 cagagacggg ctttgggtta cttcaaccag gctgaaacgc gcttcaaagc gatcggaggt
    12481 attgcgcaca aaacgtggcc acaactcact acctgggatc agttcgcgca ggttagacat
    12541 catctggcaa ccgttacata cccccagcgc cagcgtttgc ggacggtgga agaaggttgc
    12601 aaactcatcg cgtacacggt cattgaacag gattgacttc gcccaacctt caccggcacc
    12661 cagcacatca ccgtaggaga aaccaccgca cgcgaccagg gcgtggaaat cttccaggcc
    12721 cgtgcgtccg gtcagcaggt cactcatatg cacgtcgata gcatcaaagc ctgcacggtg
    12781 gaaagctgcc gccatttcaa catgcgagtt cacgccctgc tcacgcagta cggcaacttt
    12841 cggacgtgcg ccagtggcaa tatacggtgc tgccacatct tcgttgatat cgaacgacag
    12901 ttttacattc aggcccggat cggcgtcgtt agatttcgcc tgatgctcct gatcggcaca
    12961 ctccgggttg tcacgcaggc gctgcatctg ccaggtagtt tctgcccacc agacacgcaa
    13021 cgtggtgcgg ctttcgctga atacagtctg cccgttggcg gtaatcacaa aacggtcacc
    13081 ggaaaccgcc tgccctacat aatggacaca atcagcaagc ccatgctgtg ccagtacgga
    13141 ctcgaccgct tcacggtcag cggcacgaac ctgaatcacc gcacccagtt cttcgttaaa
    13201 caacgccgcc aggcgatcgt cgcccagaga ggcaatatcc gcgttaatgc cgcaatgacc
    13261 agcaaaggcc atttccgcca gcgttaccag caggccgcca tcagagcggt cgtggtatgc
    13321 gagcagctta cgctgtgcaa ccagcgcctg aatcgcgtca tagaagcctt tcagttgcgc
    13381 gacgtcgcgt acatctgccg gtttgtcgcc aagctgacga taaacctgcg ccagcgccgt
    13441 tgcgcccagc gcgttattgc ctttgcccaa atcaatcagc aatagtgcgt tatcttcggt
    13501 agaaagctgt ggcgtgatgg tgtgacgcac atcttccacg cgggcaaatg cagaaatcac
    13561 cagcgacagc ggcgacgtca tttcgcgctc ttcgttacct tcctgccagc gggttttcat
    13621 cgacatagag tctttaccca ccgggatcgt caggcccagc gccggacaaa gctcttcgcc
    13681 cacggcttta acggcttcat acaggcccgc atcttcacca gggtggcctg ccgccgccat
    13741 ccagttggcg gaaagtttaa tgcgtttgat atcgccaatc tgtgttgcgg caatattggt
    13801 taacgcttca ccgaccgcca gacgggcaga ggcggcgaaa tccagcagcg caaccggcgc
    13861 acgctcgcca atcgccatcg cttcaccgta gtagctgtcg aggctggcgg tagtgaccgc
    13921 gcagttagcg accggcacct gccacggacc caccatctga tcgcgcgcta ccatgccagt
    13981 tacgctgcgg tcaccgatgg tcaccaggaa agttttctcc gccacggtcg gcagatgcag
    14041 tatgcgtttc accgcgtctg caatggtgat cccttcacgg accagcgcat cgcccttcgc
    14101 tttcagcgtt tgtacatcgc gggtcatctt cggcgtttta ccaagcagga cgtccagcgg
    14161 cagatcgatc ggctgattat caaaatgacg atcgtgtaga gaaagatgca gttcttcggt
    14221 cgcttcacca atcaccgcgt agggtgcgcg ctcacgctta cacagttcgt caaacagcgg
    14281 taattgatcg gcagcaaccg ccagcacgta gcgttcctgg gattcgttac accagatttc
    14341 cagcgggctc atgcctggct cgtcgctcag aatgtcgcgc agttcaaatt taccgccacg
    14401 cccgccgtcg ctcaccagtt ccggcatagc gttagaaaga ccgcctgcgc caacgtcgtg
    14461 gataaacagt attgggttgg catcaccaag ctgccagcaa cggtcaatca cttcctggca
    14521 gcgacgctcc atctccgggt tgtcgcgctg tacggaagca aagtcgaggt cggcatcaga
    14581 ctgaccagac gccatggaag acgccgcgcc gccgccaaga ccgatgttca ttgccgggcc
    14641 gccgagaacg accagcttcg cgccaacgtt gatctcgcct ttttgtacgt gatcggcgcg
    14701 aatgttgccg atcccgcccg ccagcatgat cggtttgtga taaccgcgca gctcttcgcc
    14761 gttgtggctg ttcacttttt cttcataagt acggaagtag ccgttcagtg ccggacgacc
    14821 aaattcgttg ttaaacgccg caccgcccag cgggccttcg gtcatgatgt ccagcgcggt
    14881 gacaatgcgc tcaggcttac cgaaatcttc ttcccacggc tgttcgaagc caagaattcg
    14941 caggttggaa acggagaaac caaccaaacc cgctttcggc ttcgcgccgc gcccggtggc
    15001 accttcatcg cggatttcac cgccggaacc ggtcgccgcg cccggccacg gagaaatcgc
    15061 cgtcgggtgg ttgtgagttt cgactttcat cagaatatgc gccggttcct gatggaaatc
    15121 atagcggccc gtttcgtggt cagcaaagta gcggcccact tcagaacctt ccattacggc
    15181 ggcgttatct ttataagcag agagaacgtg atctggcgtg gtttcgaaag tatttttgat
    15241 catcctgaac agcgatttcg gctgttgttc accgtcgata atccagtcgg cgttaaaaat
    15301 tttgtggcgg cagtgctcag agttcgcctg agcgaacata tacagctcga tgtcgttcgg
    15361 gttacgacca agctttgtga aagcatcctg cagatagtca atttcatctt ccgccagagc
    15421 caggccaaga cgcaggttag cgtcgatcag cgcctgacgg ccctgcccca gcaaatcaac
    15481 gcttgtgacc ggagtcggtt gatggtgggc aaacaactgc tctgcatcat ctaaatcaaa
    15541 aaagacagtt tccatcatgc ggtcgtgcag ttcagcggta acctgctgcc attgttcatt
    15601 ggtcagcgta ccggcttcta tatagtaagc aacgccgcgc tcaaggcggt ttacctgttg
    15661 tagcccgcag ttatgggcaa tatcggtcgc tttcgaagac cagggagaga tggtgccagg
    15721 acgcggggtc accagcagga gtttgccttg cggggcgtgg ctggcgagtg ccggaccata
    15781 tttcagcagg cgttcaagtt gtgcgtgctc atcatcgttt aacggcgcat tgaggtcagc
    15841 aaaatggaca tactcggcgt aaatattgtg aaccgggagc ctggcagcct gaaaacgtgc
    15901 cagcagtttg ttgattcgga atgccgacag tgcaggcgaa ccacgcagaa tttccatcat
    15961 aagtctctcg tcttctaagc tttcggtgta cccaaggggg gggggaaacg ggcgtcatta
    16021 taaagaatct gatgcgctga cgaaaccgtt tgcgtggaaa taaaatcacc atcgtgaatt
    16081 agcaacgcgt gccgccaatg gctgtaataa gttgccatct ggcgcaggtt tacgcaaaat
    16141 gccgctcatt tatgagtaaa cctttcacta ttattacgtt ttttcaagct gggacgcgca
    16201 cgacacagag aattaactaa ttgaaaaaat taaagattaa ttatctgttc atcggcattc
    16261 tggcactgct gctcgcggtc gctctctggc catccattcc ctggtttggt aaagccgaca
    16321 accgtatcgc cgccattcaa gcgcggggag agttgcgtgt gagcaccatt catactcccc
    16381 tgacttataa cgaaatcaac gggaaacctt ttggcctgga ttacgaactg gcgaaacagt
    16441 ttgccgatta cctcggcgta aaactgaaag tgaccgtgcg gcagaatatc agccagctgt
    16501 ttgacgacct tgataatggt aacgccgacc tgctggcggc aggactggtc tataacagtg
    16561 agcgggtaaa aaattatcag cctggcccta cctattattc cgtgtcacaa caactggttt
    16621 ataaagtggg tcagtatcgc ccacgtacgc tgtgcaacct gacggcggag caactcaccg
    16681 ttgcaccggg tcatgtggtg gttaacgatc tccagaccct gaaagaaaca aaattcccgg
    16741 aattaagctg gaaggtagac gacaaaaaag gctctgcgga attaatggaa gatgtcatcg
    16801 aaggaaaact cgattacacc attgctgatt ctgtcgccat cagcctgttt cagcgcgttc
    16861 acccggagct cgccgtagcg ctcgatatca ccgatgaaca accggtgact tggtttagcc
    16921 cgttagatgg cgataatacc ctttccgccg ccctgctcga cttcttcaac gaaatgaatg
    16981 aagacggtac gctggcacgc attgaagaga aatacctggg gcatggcgat gattttgatt
    17041 acgtcgatac gcgcacattt ttacgcgccg tcgatgcggt actgccgcag ttaaagcccc
    17101 tgtttgagaa atacgccgaa gaaattgact ggcgtttgct ggccgctatt gcttatcagg
    17161 aatcgcactg ggatgcacag gccacttcac cgacgggtgt gcgcggcatg atgatgttaa
    17221 ccaaaaatac cgcgcaaagc ctcggcatta cggatcgtac cgatgccgaa cagagcatca
    17281 gcggtggcgt gcgttatttg caggatatga tgagtaaagt gccggaaagt gtgccggaga
    17341 acgagcggat ctggtttgcc ctcgctgcgt acaatatggg ctatgcgcat atgctggatg
    17401 cccgcgccct gacggcaaaa accaaaggga atcctgacag ttgggctgac gtaaaacagc
    17461 gtctgccttt acttagccag aaaccctatt acagcaagct gacttacggc tacgctcgtg
    17521 gacatgaagc ctacgcttat gtcgaaaata ttcgtaagta tcaaattagc ctggtgggtt
    17581 atctgcaaga gaaagagaag caggctacag aagcggcgat gcaactggcg caggattatc
    17641 cggcggtatc gcctacggag ttgggcaaag agaaatttcc ttttctctcg tttctttccc
    17701 agtcgtcatc aaactatttg acccattctc cctctctgct gttttccagg aaagggagtg
    17761 aagagaaaca aaattaatcc gtcgaggatt gcgctttttt ctgcgcttta atttcctggc
    17821 ggcgcatgcg aaagaagtca ctgagcaacg ccgcgcactc atccgccagt attccttccg
    17881 taatttccac tcggtgattc atacccggat gatgcagcac atccattaaa gatcccgcag
    17941 cgccagtttt cgcgtcacgc gcaccaaaga ccacgcgacc aatgcgactg tggatcatcg
    18001 ctccggcaca cattacacat ggttcaagcg tgacatacaa cgtggcgtcg atcagacgat
    18061 aattttgcat caccagacca ccctgccgca gggccatgat ttctgcatgt gcggtgggat
    18121 catggcgacc aatcgggcgg ttccaacctt cgccgattac ccgattgtta tgcactaata
    18181 ccgcgccgac cggcacttcc cgctcatccc aggcacgttt cgccagcgtc agcgcgtgac
    18241 gcatccagta ttcgtggcta aattcgactt cagacaaaaa gaaaactccg gttataaaag
    18301 cgcggcgcat tatacacgga cgctatgctt tactctattc cagttgctgg agttcaccgc
    18361 gcggggttac tcgccaacga tgctgacaga aataaagcag cggattgtcc tgattactgt
    18421 cgctatagcc actgtacagc cgcagcggag tgccgatttt gcgctccagt tgtgcgacct
    18481 tttcatgtcc cagacaacgc atcgtcaata cccaaccacc atagccacgc tgaatttggc
    18541 tggcgataag attaacccgc ggcagccagg gcttatcgaa ataaaccgct tcaaccagcg
    18601 gctgcggaga gccggtaatc aaccagatat cagcatcgga acttaacagg taggtggtta
    18661 atcgctcctg aaccagcgga aaggcggtaa cattgtcgcg aaaccagcgc acgaaatcgg
    18721 cctgcaacgt ctgtaaacgt gcttcgctgt gaccaaaagt gcacccccac agaagcagac
    18781 tcatcggcca gcgtgccgca cgacctttta tcaataacgc aatggctata atcggtaaca
    18841 acggcaggac aagtaacgca ttcagcggtt ggcgacgtag taaatagcgc agaaaactgc
    18901 cgaacatatc ctgctgatgc aatgttccat ctaagtcaaa aaacaccaca cgacgctcgt
    18961 gagttgccaa accatactcc tctggactga aacatcctga ttaattttcg ctactacata
    19021 gcctaacaga tagatcatca cttttccggc aacaatcgac cgcatcatgg ctaactggaa
    19081 tttttaattc atggcaatta gcggcaatgg aatataaaat tcactcgcgt gtgtctcata
    19141 ttgcctgttg tcgccactat ttcatcgcga ggaaagatat gaacggctta cttcgtatcc
    19201 gtcagcgtta ccaggggctt gcccagagtg ataaaaaact ggcggattat ctgctgctac
    19261 aacctgatac ggcgcgccat ttaagctctc agcaactggc caacgaagcc ggagtcagtc
    19321 agtccagcgt cgtgaagttc gcgcaaaaac tcggctataa aggttttccg gcgcttaagt
    19381 tggcgttgag tgaagcgctg gcaagccagc cggaatcgcc ctccgtgccc attcataacc
    19441 aaatccgcgg tgatgatccg ttacggctgg tcggcgaaaa actgattaaa gaaaatactg
    19501 ccgctatgta cgcaacgcta aacgttaata gtgaagagaa actgcatgaa tgcgtaacaa
    19561 tgttgcgctc tgcgcggcgg ataattctga ccggtattgg cgcttcgggt ctggtggcgc
    19621 aaaactttgc ctggaagctg atgaagattg gcttcaatgc tgccgcagtg cgcgatatgc
    19681 atgcgctact cgcaacagta caggcgtcgt cccctgacga tctgttatta gccatttcct
    19741 acaccggtgt acgacgcgag ttaaacctgg cggcagatga gatgctgcga gtgggcggaa
    19801 aagtgctggc gattaccggc tttactccga atgccctgca acagcgtgct tctcattgcc
    19861 tgtataccat tgccgaagaa caagcgacaa acagtgcttc aatctctgct tgtcacgctc
    19921 agggaatgtt aacggatttg ctgttcattg cgctgattca gcaggatctg gaactggcac
    19981 cagaacgtat tcgtcatagt gaagcgctgg tgaaaaaact ggtctgagta aagaatgcgc
    20041 gtataatgcc cgcccggttt gtgttgtttt gagagtttcc ttatggcgtt gttaatcact
    20101 aaaaaatgca tcaattgtga tatgtgtgaa cccgaatgcc cgaatgaggc gatttcaatg
    20161 ggagatcata tctacgagat taacagcgat aagtgtaccg aatgcgtagg gcactacgag
    20221 acaccaacct gccagaaggt gtgcccgatc cccaatacta ttgtgaaaga tccggcgcat
    20281 gtcgagacag aagaacagtt gtgggataaa tttgtgctga tgcaccacgc ggataaaatt
    20341 taatattcta ctctggaagt agaggattaa ttatattact gggaagcctt aacgccatta
    20401 tatttattta attgatgaca ttagcataat cattcactaa gttaatttat atagtatctg
    20461 ccaagacact tatttatagt tattaaaggc gcgtccgatt ggttcaccgg acgcacctta
    20521 agtacgtttc cttgtgttat aagaacagaa ggatcagctg taaaacagca atgatgattt
    20581 tgatgacccg tttaatcagg tatcggcaat cagtcattcg tttttcctta aacaaggaat
    20641 gcagccgttc agattagccc ttacatctcc ccaaaactga acgtgcgagt tattgagggt
    20701 gcatgctgca ctccacacca gagctttgac gacaccactc gtttcaatgg gggaattctg
    20761 tggcatggtg taaagcacag caaaatcttc aataacgaag ccaattttaa tgtacttacg
    20821 aattggcggt cacgttaatc tttccatcaa tattgctttc ttcgtaaagg ctcgagtttt
    20881 tatgctaaag attgcaagtt gcttgtaaaa gataagtaca ctgatccata atcgctgttg
    20941 ttgagggtgc atgctgcaca aaattaaagt taaaaagtaa aacccccgtt ccttaccagt
    21001 tcgggggttt tactttttaa agagaacggt attattttaa ctttcaataa ttaccgtggc
    21061 acaagcatag tggcgttcat ctgccagcgt tacatgcata tttacaacgc cgagcttttc
    21121 cgccagtttt aatgcctcac cccatagccg tagccgtggt ttgccgagct catcattgaa
    21181 tacttcaaat tgattaaacg ccagaccatt gcggatcccg gtgccaaacg cttttgctgc
    21241 ggcttctttc acagcaaaac gcttcgccag aaaacgcacc ggctggtggt gcgttttcca
    21301 gattgcccat tcgttatcgc ttaatacgcg gcgtgccagg cgctcaccgg atcgggcgat
    21361 cactgcttct atgcgagcga tctctacgat atccgtgcct aaacctaata ttgccattag
    21421 ccacgcgctt ccagcatcag acgcttcatt tctgccaccg catctttcag tccggtcatc
    21481 actgcacggc caataatggc atgaccgata ttcagttcat gcatctcagg gatggcggca
    21541 atggctttca cgttgtgata ggtcagaccg tgtccggcgt taactttcag accgagacta
    21601 gcggcaaagg tcgcggcttt ggcgatacgc accagctctt gcgcctgttc ggcgtcagtt
    21661 ttggcatcag catagcaacc ggtgtggatc tcgataaacg gtgcgccaac ctctgccgca
    21721 gctttgatct gctcttcatc ggcgtcgata aagagagaaa cctgaatccc ggcatctgtc
    21781 agacgtttgc aggcatcgcg cattttttca cgctgccctg caacgtccag gccgccttcg
    21841 gttgttactt cctgacgctt ttccggtacc aggcagcaaa aatgtggctt cgtctcaacg
    21901 gcgatcgcca gcatctcttc ggtcactgcc atctccagat tcattcgggt atccagcgtc
    21961 tgccgcagga tgcgcacatc gcggtcagta atgtggcggc gatcttcacg taaatgcacg
    22021 gtaatgccgt ccgctcccgc ctgctcggca ataaacgccg cctgcaccgg atccgggtaa
    22081 gcagtaccgc gtgcgttacg cagcgttgca atatggtcaa tgttgacgcc taacagtaat
    22141 tcagccatga caatcctcat cattcataat gtgttttcac cgttcgctta ggcataaact
    22201 gccggaacag ttccctgctc tttaatggtt taccgccaag atacggctta agcgccatgc
    22261 gggtaaagcg tttcgcggcg cgtagtgtgt ctgcgtcagg aaattcccgc gcgtttaacg
    22321 cttttaactg ccttccggtg aacgttttat tgtcgataac gacgcttgcg ataaaccctt
    22381 tttcttcgcg ataacgatac gtcatggtgt catctaccgg ctcgccgcta cccgcgcaat
    22441 aggtaaaatt gacgccatag cccagatgcc cgagcagtgc cagttcaaag cggcgcagcg
    22501 cgggttctgg cgtaccagtg gcacctgcaa gagactgaat gcagtgcaaa taatcgaaaa
    22561 agagttcaga gaagcgcgtc tcgtattcca gtacgcggga gagaagttcg ttgatgtaca
    22621 gaccgctgta aagcgtgata ccgcttaatg gcagcgccag cgagacagct tcagcactgc
    22681 gcagcgtttt gacttcgcca cgcccgccga aacgtagcaa gagaggggtg aaaggctgta
    22741 atgcaccttt cagagtagag cgtttagagc gtgcgccttt ggcaaccaga cgcacgcgac
    22801 ccgattcctc cgtgaagacg tccagcatca ggctggtttc gctccacggg cgactatgca
    22861 ggacaaatgc gcgctgccag ccttccatcg gagtgactct taaagatcgt caacgtaacc
    22921 gagactgcgc agtgcgcgtt cgtcgtcggc ccaaccggat ttcactttta cccacagttc
    22981 aaggtggaca ggcgcttcga acatttcctg catgtcttta cgcgcttcaa tcccgatggt
    23041 tttgatcttg gcccctttgt tgccaataac catcttcttc tgcccttcac gctcaacgag
    23101 aatcaaacca ttgatgtcat aaccaccgcg ttcgttagag acgaaacgtt cgatctccac
    23161 ggtcacggag tacggcagtt cagcgccgag gaaacgcatc agtttttcgc ggatgatttc
    23221 agacgccata aaacgctgtg agcgatcggt gatgtaatct tccgggaagt gatgggtcgc
    23281 ttcaggcaga tgcttacgca cgattgctgc gatagtgtcg acattcagcc cggtttcagc
    23341 agagatcggc acgatatcga ggaagttcat ctgacttgcc aggaactgta agtgcggcag
    23401 cagatcggct ttttcctgca cgttatccac tttgttcacc gcgaggatca ccggcgcttt
    23461 gccatcgcgc agtttgttga gcaccatttc gtcgtccggc gtccagcggg tgccttcaac
    23521 gacaaaaatc accagctcaa cgtcgccaat agagctgctc gccgctttgt tcatcaggcg
    23581 gttaatagcg cgtttttctt ccatatgcag gccaggggta tcaacgtaga tcgcctgata
    23641 cgcgccttca gtatggatcc ccacaatgcg gtgacgagtt gtttgcgctt tgcgggaggt
    23701 gatggagatc ttctgcccca gcagtttgtt caacaatgtg gatttgccaa cgttcggacg
    23761 tccgacgatg gcaataaatc cgcagtaact tttatcgatg ctcattccag ctccagtttt
    23821 ttcaacgcct gttcggcggc agcctgctca gccttacgac ggcttgaacc tgtgccaacc
    23881 accggttcac tcaggccgct gacctggcag tggatagtaa attcctgatc gtgcgcttcg
    23941 ccacgtacct ggactaccag ataagtcggc agcggcagat ggcgaccctg caaatattct
    24001 tgcaagcgcg ttttcggatc tttttgttta tcgcctgggc taatttcgtc caaacgagtt
    24061 tgataccagt tgaggattaa tttctcgacg gtttgaatat cactgtcgag gaatacgcca
    24121 ccaattaatg cttcgacggt atcggcgaga attgactcac gacgaaatcc accgctttta
    24181 agttcaccag gccctaaacg taagcactcg cctaactcaa attcgcgcgc cagttccgcc
    24241 agcgtattgc cacggaccag cgtggcgcgc atccggctca tatcgccttc atccacacga
    24301 gggaaacggt gataaagcgc attggcgata acgtagctca gaatagagtc gcctaaaaat
    24361 tctaaacgct cgttatgttt gctgctggca ctacgatgag ttaatgcctg ctgcaacagt
    24421 tcctgatgat taaaagtgta gcccagcttc cgttgaagcc gattaattac gatggggttc
    24481 atgcgatacc aataaataaa tgcgtcaaca attcagcaca cgaaacagac ctgatataca
    24541 tggctctgct aactgcttcg ctgcagtttc agtttaccgg tatatgggga ccaacgctgt
    24601 ttcgtgtgcc gtggcaacct ggaggtgcca accttaaact tcgggggaat attctataca
    24661 caacgacggg ggatgtcgtt agccacggga gatttatctc ataaataatt cacgttgtcg
    24721 ccataacggc gacaacgtga acgaagatgg gctattaatg gatgccgcca atgcgactta
    24781 agcgcacacc tgtcggccat tcgccttctt gcttatcgaa actcatccag atagccgttg
    24841 cccgaccgac cagattcgct tcaggcacaa aaccccagta acggctgtcc gcgctgttgt
    24901 cgcggttgtc gcccatcatg aagtattgtc ccggcggaac aatccaggtt gccagttgtt
    24961 gccctggctg ttggtaatac atccccacct ggtcctgcgc aatcggcact gtcagaattc
    25021 ggtgcgtcac atcacccagt gtctctttac gctcggaaag acgaattcca ttttctttgg
    25081 tttcgttttt cggcacttca aagaatccgc tggtcgcttc cccaccatta cggcgtgaga
    25141 aggtctgaac gaaatcgctc ggttccacgt tggagtaggt gaccggtagc gcgttttcac
    25201 acgcctggcc ggaactgcat cccggttgaa tcgtcagctc ttttgagacc ggatcgtaag
    25261 tgactttatc gcccggtaaa cccaccgcgc gcttgatgta atcaagcttt ggatcttccg
    25321 gatatttaaa gaccacgata tcgccgcgtt tcggatgacc ggtttcgatc agcgttttct
    25381 ggtagatagg atctttaatg ccataagcaa acttctctac cagaataaaa tcaccgatta
    25441 acagagtcgg catcatcgaa cctgacggga tctggaacgg ttcataaata aacgaacgta
    25501 caatcaatac gatagccagc accggaaaaa cagaagcacc ggtttccagc cagccaggct
    25561 tcggcgcaac ctttttcagg gttgctttat ccagtgagtc accggcagcc gcctgcgccg
    25621 ctgcctgacg ttcccgccgt ttaggtgcga aaaagaattt atccacgcac cataaaatgc
    25681 ccgtcaccag tgtggcaatc accagaatca gggcaaacat attcgccatg ccaactccta
    25741 agggttattt gttgtctttg ccgacgtgca gaatggcgag gaacgcttcc tgcggcagct
    25801 cgacgttacc gatctgcttc atgcgtttct taccttcttt ctgcttctgc agcagctttt
    25861 tcttacggct gatatcgccg ccataacatt tagccagtac gtttttacgc agctgtttca
    25921 cggtggatcg cgcaataatg tgcgtaccaa tcgctgcctg aatggcgata tcaaactgct
    25981 ggcgtgggat cagatctttc atcttctcca ccaactcgcg accgcggttt tgcgaattat
    26041 cacggtgggt gatcagcgcc aacgcatcaa cacgttcacc gttgattaat acgtctacac
    26101 gtaccatgtc ggacgcctgg aagcgtttga agttgtaatc cagagacgca taaccacgcg
    26161 aggtagattt caggcgatcg aagaagtcga gcaccacttc cgccatcggg atctcgtacg
    26221 tcagcgccac ctgattaccg tggtaaacca tattggtctg cacgccgcgt ttttctacgc
    26281 acagcgtaat aacgttgccg agatatgcct gcggcagcag catgtgacac tctgcaatcg
    26341 gctcgcgcag ttcgtaaatg ttatttaccg caggcagctt ggatgggctg tcgacgtaga
    26401 taacttccct tgacgtggtt tcaacttcat acactacggt cggcgcagtg gtgatcagat
    26461 ccagatcgta ttcacgttcc agacgttcct ggatgatctc catgtgcagc aggccaagga
    26521 agccgcagcg gaaaccaaag cccagcgcgc tggagctttc cggctcatag aacagtgagg
    26581 catcattcag gctgagttta cccagcgcgt cacggaaggc ttcatagtcg tcggaactta
    26641 ccgggaacag accggcgtat acctgcggtt tgactttctt aaagccaggc agcgcctttt
    26701 ctgccggatt acgcgccagc gttaaggtat cgccgactgg agcgccgtgg atatctttaa
    26761 tcgcacatac cagccagcct acttcgccac atttcagttc agtgcggtca acctgtttcg
    26821 gcgtgaagat gcccagacgg tcggcgttat aggtctgccc ggtactcatg actttcactt
    26881 tgtcgccctt acgcagggtg ccgtttttaa tacggataag tgaaacaacg cccaggtagt
    26941 tgtcgaacca tgagtcgata attagtgcct gcaacgggcc ttccggatcg ccttccggcg
    27001 gcggaatgtc gcgcaccaga cgttcgagaa cgtcctgcac gccaacgccg gttttcgctg
    27061 aacagcgcac cgcgtcagtg gcgtcgatgc cgacgatatc ttcaatttct tccgccacgc
    27121 gttcaggatc ggctgccggc aggtcaatct tgttcagtac tggcaccact tcgagatcca
    27181 tttccatggc ggtgtagcag tttgccaggg tttgcgcttc cacgccctgc ccggcgtcga
    27241 ccaccagcaa tgcaccttca caggcagcca gcgaacggga aacttcatag gagaagtcta
    27301 cgtggcccgg ggtgtcgata aagttaagct gataggtttc gccgtcagac gctttgtagt
    27361 ccagcgtcac gctttgcgct ttgatggtaa tgccacgctc acgctcaaga tccatggaat
    27421 cgagaacctg cgcctccatt tcacggtcag acaggccacc gcagatctgg ataatacggt
    27481 cagacagcgt cgatttaccg tggtcaatgt gagctatgat cgaaaagtta cgtatattct
    27541 tcataaagta tgattattgt gccttaatgc ccggttaacc aggcttttag aagtcgctgt
    27601 tctgagctta acgtctgtat taatagaaac gccgcattct acactacaac attgaggcga
    27661 ggaaatgttc ataccgtatg gattgtggta tctggaaacg tcctcgcatt tgttatgcaa
    27721 aatgcaacaa agccagtgaa atcactggct cgcgtcttcc gaagatgttt caaatcgcac
    27781 aaggccaggc ggcaatgcca cgcttaagat gatcggttgc cattctgccc gggcagcaaa
    27841 cttacgcgag tagccgcgcg caatcaggaa cccgccaata ccgccgagga ccgcaccaca
    27901 taatgctgcg acgtcagaag caaagagtag ctgaaatagc gaagcgatga ggaataatcc
    27961 caccagcggc gacatataaa ccagtaatgc ggagctaagc aggctgcctt cggcgatccc
    28021 caattccact ttttgccccg gcactaacgg ttcatcacag ggtacgacaa tggtatgcgt
    28081 ggtttgcggg ccaagtttat tcaacacgcg gctaccgcaa ccggcgcgtg aagcgcagct
    28141 gctgcatgaa gctttaacat cacaactgac cagcgcctgc ccgttttgcc aggagacgac
    28201 ggtagcccac tctttgatca ttgcgctgcc ccgaacttaa tattctcggc aatgcgtttc
    28261 gccgtttgcg gcggcagttc accgacaatg gtgatttcgg cgttatcacg tacgcttgta
    28321 ctgacggttc tgcgtccggt gcgcaacatc tgatcggtgc tcgatggcgt agcgcgatta
    28381 acgtttaccg agaagctgaa taatccgtca gaatagagac gtgattcgat aggcatgttg
    28441 tccatcgtcg gtagcggacg tcgactactg gaaacttcgc taaaaccctg tggcaaccag
    28501 gttggcgtcc agctgaattt agctttttca cctacaggaa cagaaagcaa cggcggcaaa
    28561 tttgccttcg ccagcgtctg catactgctg ctgatatcct gattgacgtt aaaagcaatc
    28621 acgcgaaatt gttccagcgt ttcaccatcg cgatcaagga gatcaacccg catcggtaat
    28681 ttcgattcgg tgtccatcca cacgatgtag ctgtagcgtg taccatctcg ggcaaccacg
    28741 cgaatgactt cgcaaagacg atcagcaata cgcgtgcgcc cgacggagat aaagtcgtag
    28801 taaggagaaa ggcgtttgaa atcggtatag atgagcgatg gcagagaatc aacaatgtaa
    28861 tcgccattaa gcgtgaacgg ttcaagtccc ggttcaaaat agctgatttc atcgccgcgc
    28921 tgtaccactt cccggcgcgg gccatccatt tgcaacaatt gtgcaagagg acggttatcg
    28981 aggcgtgcat gtcgataacg cagagactca acaccctgtt tattgatgct gatgaatgac
    29041 agctcgtaat tcagtgactg actggccagg ttcatctgct gtaataacgc cccggacgcg
    29101 ggagtggccg aggcgttagc agagaataac aggctacctg tcactaatga catggcaaac
    29161 caaagttgct tcattactgc gattgcgttc ctaaagtttg aattcctggc acctgtacag
    29221 cggcttgctg ggtttgcgcc tgctcaaact gaagctgttc agagtggagt cggcgttgca
    29281 gttcgtaatc ctgcaacatt gcattaatgc gacgacgctg ctcctgtacc tgctgctgtt
    29341 gaccattgtt tgcggtcgct tcagaaggta ctcccaggct taccgggctg gctttaccca
    29401 tcatcggcag tgtattaaat accggcgttt cgggctgctg ggacgtttca gattgtccat
    29461 tatagtgctg gacgccaacg ataactgcaa gcgatacgca tgctgctacg cccatttggg
    29521 taagctgtgc cgcccacgga cgtactttct gccagaatgg cattttctgc cattgatgcg
    29581 gcgcaggctg ggcttccggg atcaatgtcg ccggttgacg tactggctct tcttcaatgg
    29641 cggccatcac gcgtgaagag atatcgaaat ggagcacctc gggagtatca ccccgcattg
    29701 agtcacggat taagtgatag ctttcccagg ttttctgcat ttctgggtta tgagccagtt
    29761 cgttaagcag ctcactatcc agcgtttcgc catccattaa agcggaaagt tgttctttct
    29821 gcatgcctaa tacccttatc cagtatcccg ctatcgtcaa cgcctgataa gcggttgaac
    29881 tttgttatca atagcttccc tcgctcggaa gatacgtgaa cgcaccgtac ctaccggaca
    29941 atccatgata gcggctatct cttcatagct caggccatcc agctcccgca aggttattgc
    30001 catgcgcaaa tcttccggga gggactcaat agttcggaaa actatctgtc tcagttcttc
    30061 tgacaacatt aagttctcag ggttcgaaat ttctttcaac gcgccgccac tttcgaagtt
    30121 ttcagcttca atggcatcca catcactgga aggtggacga cgcccctgag caaccaggta
    30181 atttttcgct gtatttacag caatccgata cagccatgta taaaaagcgc tatctccccg
    30241 gaacgaatcc agcgcacgat aggctttaat aaaagcttct tgtaccacat cgggaacatc
    30301 acccgacggc acatagcggg aaaccagact cgccacttta tgctgatagc gcactaccag
    30361 taagttaaag gctttctgat ctcccttctg gacccgttca accaggacct ggtccgttaa
    30421 ctgctcgctc atccgaggta aagtctcccc aaaccaaatt tccacgcgct atcgaaacgc
    30481 cactccatta gctgcaattt gagcaagcaa agggttagag tgtctcgttt ttgtaaagtt
    30541 ccgtaacgca tctgtttttg tttgtcatgc tgtagacgga tcattatcta tcattataag
    30601 tctacagaat ctgaacatcg cattatctgt gtagaaatgc ccatttaact gcctgaagag
    30661 taacccaacg gcctttttat ttcaccacct aatcctccac cagccagtaa cttctctttt
    30721 tctcgccgcc ctgcatcagc gtgtttagca actgtaacaa atattaaaat agcaggtgtt
    30781 tattcgcaca acatgatgct atgctgacca aaccgtgttt agtaaattaa acaaagaaaa
    30841 tgaatactct ccctgaacat tcatgtgacg tgttgattat tggtagcggc gcagccggac
    30901 tttcactggc gctacgcctg gctgaccagc atcaggtcat cgttctaagt aaaggcccgg
    30961 taacggaagg ttcaacattt tatgcccagg gcggtattgc cgccgtgttt gatgaaactg
    31021 acagcattga ctcgcatgtg gaagacacat tgattgccgg ggctggtatt tgcgatcgcc
    31081 atgcagttga atttgtcgcc agcaatgctc gttcctgtgt gcaatggtta attgaccagg
    31141 gggttttgtt tgatacccac gttcaaccga atggcgaaga aagctaccat ctgacccgtg
    31201 aaggaggaca tagtcaccgt cgtattcttc atgccgccga cgccaccggt agagaagtac
    31261 aatccacgct ggtgagcaag gcgcagaacc atccgaatat tcgtgtgctg gagcgcagca
    31321 acgcagttga tctgattgtc tctgacaaaa ttggcctgcc gggcacgcga cg
//
LOCUS       DAZPEB010000026        30870 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZPEB010000026 DAZPEB010000000
VERSION     DAZPEB010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30870
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.026"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            248..631
                     /gene="secE"
                     /locus_tag="ACHURJ_001070"
     CDS             248..631
                     /gene="secE"
                     /locus_tag="ACHURJ_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFR1280107.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            633..1178
                     /gene="nusG"
                     /locus_tag="ACHURJ_001071"
     CDS             633..1178
                     /gene="nusG"
                     /locus_tag="ACHURJ_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFR1280108.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1338..1766
                     /gene="rplK"
                     /locus_tag="ACHURJ_001072"
     CDS             1338..1766
                     /gene="rplK"
                     /locus_tag="ACHURJ_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFR1280109.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGAGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1770..2474
                     /gene="rplA"
                     /locus_tag="ACHURJ_001073"
     CDS             1770..2474
                     /gene="rplA"
                     /locus_tag="ACHURJ_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFR1280110.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2887..3384
                     /gene="rplJ"
                     /locus_tag="ACHURJ_001074"
     CDS             2887..3384
                     /gene="rplJ"
                     /locus_tag="ACHURJ_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFR1280111.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3451..3816
                     /gene="rplL"
                     /locus_tag="ACHURJ_001075"
     CDS             3451..3816
                     /gene="rplL"
                     /locus_tag="ACHURJ_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFR1280112.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4136..8164
                     /gene="rpoB"
                     /locus_tag="ACHURJ_001076"
     CDS             4136..8164
                     /gene="rpoB"
                     /locus_tag="ACHURJ_001076"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFR1280113.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8241..12464
                     /gene="rpoC"
                     /locus_tag="ACHURJ_001077"
     CDS             8241..12464
                     /gene="rpoC"
                     /locus_tag="ACHURJ_001077"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFR1280114.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQML
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            12677..13216
                     /locus_tag="ACHURJ_001078"
     CDS             12677..13216
                     /locus_tag="ACHURJ_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-shock protein C"
                     /protein_id="HFR1280115.1"
                     /translation="MKQEVEKWRPFGHPDGDIRDLSFLDAHQAVYVQHHEGKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKQSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVAFRAFREKKKLRLHVTSAYPISEKQKGKSVK
                     FFTIAYNLLRNKQLPQPSK"
     gene            complement(13626..14759)
                     /gene="thiH"
                     /locus_tag="ACHURJ_001079"
     CDS             complement(13626..14759)
                     /gene="thiH"
                     /locus_tag="ACHURJ_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFR1280116.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAVDVERALNASQFTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAKTEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14756..15526)
                     /gene="thiG"
                     /locus_tag="ACHURJ_001080"
     CDS             complement(14756..15526)
                     /gene="thiG"
                     /locus_tag="ACHURJ_001080"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFR1280117.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAEMLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(15528..15728)
                     /gene="thiS"
                     /locus_tag="ACHURJ_001081"
     CDS             complement(15528..15728)
                     /gene="thiS"
                     /locus_tag="ACHURJ_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFR1280118.1"
                     /translation="MQILFNDQPMQCATGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHILQDGDQILLFQVIAGG"
     gene            complement(15712..16467)
                     /gene="thiF"
                     /locus_tag="ACHURJ_001082"
                     /pseudo
     CDS             complement(15712..16467)
                     /gene="thiF"
                     /locus_tag="ACHURJ_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
     gene            complement(16460..17095)
                     /gene="thiE"
                     /locus_tag="ACHURJ_001083"
     CDS             complement(16460..17095)
                     /gene="thiE"
                     /locus_tag="ACHURJ_001083"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFR1280119.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVISAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17095..18990)
                     /gene="thiC"
                     /locus_tag="ACHURJ_001084"
     CDS             complement(17095..18990)
                     /gene="thiC"
                     /locus_tag="ACHURJ_001084"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFR1280120.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYLTGTHSGVRV
                     PMREIQLSPTLIGGSKEQPQFEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19071..19171)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19224..19700)
                     /gene="rsd"
                     /locus_tag="ACHURJ_001085"
     CDS             complement(19224..19700)
                     /gene="rsd"
                     /locus_tag="ACHURJ_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFR1280121.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQKVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19795..20568
                     /gene="nudC"
                     /locus_tag="ACHURJ_001086"
     CDS             19795..20568
                     /gene="nudC"
                     /locus_tag="ACHURJ_001086"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFR1280122.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVVLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20608..21672
                     /gene="hemE"
                     /locus_tag="ACHURJ_001087"
     CDS             20608..21672
                     /gene="hemE"
                     /locus_tag="ACHURJ_001087"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFR1280123.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKVTRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21682..22353
                     /gene="nfi"
                     /locus_tag="ACHURJ_001088"
     CDS             21682..22353
                     /gene="nfi"
                     /locus_tag="ACHURJ_001088"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFR1280124.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLMVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22396..22986
                     /locus_tag="ACHURJ_001089"
     CDS             22396..22986
                     /locus_tag="ACHURJ_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFR1280125.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVVLSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQVGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            23173..23445
                     /gene="hupA"
                     /locus_tag="ACHURJ_001090"
     CDS             23173..23445
                     /gene="hupA"
                     /locus_tag="ACHURJ_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFR1280126.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23458..24153
                     /locus_tag="ACHURJ_001091"
     CDS             23458..24153
                     /locus_tag="ACHURJ_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFR1280127.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALLLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24155..24580)
                     /gene="zraP"
                     /locus_tag="ACHURJ_001092"
     CDS             complement(24155..24580)
                     /gene="zraP"
                     /locus_tag="ACHURJ_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFR1280128.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24818..26194
                     /gene="zraS"
                     /locus_tag="ACHURJ_001093"
     CDS             24818..26194
                     /gene="zraS"
                     /locus_tag="ACHURJ_001093"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFR1280129.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIVQHGVISVTVSESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            26191..27515
                     /gene="zraR"
                     /locus_tag="ACHURJ_001094"
                     /pseudo
     CDS             26191..27515
                     /gene="zraR"
                     /locus_tag="ACHURJ_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
     gene            complement(27512..28801)
                     /gene="purD"
                     /locus_tag="ACHURJ_001095"
     CDS             complement(27512..28801)
                     /gene="purD"
                     /locus_tag="ACHURJ_001095"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFR1280130.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDDVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACESKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLLLEEVAGGKVFHAGTKLADDEQVVTNGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28813..30402)
                     /gene="purH"
                     /locus_tag="ACHURJ_001096"
     CDS             complement(28813..30402)
                     /gene="purH"
                     /locus_tag="ACHURJ_001096"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFR1280131.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7315 a         7971 c         8420 g         7164 t
ORIGIN      
        1 gcaaaagttc tgagctaatt gccgataaca tttgactcaa tgcgcactaa aagggcatca
       61 tttgatgccc tttttgcacg ctttcgtacc agaacctggc tcatcagtga ttttctttgt
      121 cataatcatt gctgagacag gctctgttga gggcgtataa tccgaaaagc taatacgcgt
      181 ttcgatttgg tttgcctcgc gatcgcgggg tgaaaatgtt tgtagaaaac ttctgacagg
      241 ttggtttatg agtgcgaata ccgaagctca aggaagcggg cgcggcctgg aagcgatgaa
      301 gtgggtcgtt gtggtggcat tgctcttggt ggcgattgtc ggcaactacc tttatcgcga
      361 cattatgctg ccgctgcgtg cgctggccgt agtaattctg attgctgcag cgggtggtgt
      421 cgcgctgtta acgacaaaag gtaaagctac cgttgctttt gcccgtgaag cgcgtaccga
      481 agtccgtaag gtcatttggc cgactcgcca ggaaacattg cacaccacgc tgattgtggc
      541 tgcggttacc gcagtaatgt cactgatcct gtggggactg gatggtattc tggttcgcct
      601 ggtatccttt atcactggcc tgaggttctg agatgtctga agctcctaaa aagcgctggt
      661 acgtcgttca ggcgttttcc ggttttgaag gccgcgtagc aacgtcgctg cgtgagcata
      721 tcaaattaca caacatggaa gatttgtttg gtgaagtcat ggtaccaacc gaagaagtgg
      781 ttgaaatccg tggcggtcag cgtcgcaaaa gcgaacgtaa attcttccct ggctacgtcc
      841 tcgttcagat ggtgatgaac gacgcgagct ggcacctggt gcgcagcgta ccgcgagtga
      901 tgggcttcat cggcggtact tccgatcgtc ctgcgccaat cagcgataaa gaagtcgatg
      961 cgattatgaa ccgcctgcag caggttggtg ataagccgcg tccgaaaacg ctgtttgaac
     1021 cgggtgaaat ggtccgtgtt aatgatggtc cgttcgctga cttcaacggt gttgttgaag
     1081 aagtggatta cgagaaatct cgtctgaaag tgtctgtttc tatcttcggt cgtgcgaccc
     1141 cggtagagct ggacttcagc caggttgaaa aagcctaacc cagcgatcaa aaaagcggcg
     1201 atttaatcgt tgcacaaggc gcgagattgg aatacaattt cgcgcctttt atttttatgg
     1261 gccttgcccg taaaaacgat tttttatatc acggggagcc tctcagaggc gttattaccc
     1321 aacttgagga atttataatg gctaagaaag tacaagccta tgtcaagctg caggttgcag
     1381 ctggtatggc taacccgagt ccgccagtag gtccggctct gggtcagcag ggcgtaaaca
     1441 tcatggaatt ctgcaaagcg ttcaacgcaa aaactgattc catcgaaaaa ggtctgccga
     1501 ttccggtagt aatcaccgtt tacgctgacc gttctttcac tttcgttacc aagaccccgc
     1561 cggcagcagt tctgctgaaa aaagcggctg gtatcaagtc tggtgccggt aagccgaaca
     1621 aagacaaagt gggtaaaatt tcccgcgctc agctgcagga aatcgcgcag accaaagctg
     1681 ccgacatgac tggtgccgac attgaagcga tgactcgctc catcgaaggt actgcacgtt
     1741 ccatgggcct ggtagtggag gactaagaaa tggctaaact gaccaagcgc atgcgtgtta
     1801 tccgcgagaa agttgatgca accaaacagt acgacatcaa cgaagctatc gcactgctga
     1861 aagagctggc gactgctaaa ttcgtagaaa gcgtggacgt agctgttaac ctcggcatcg
     1921 acgctcgtaa atctgaccag aacgtacgtg gtgcaactgt actgccgcac ggtactggcc
     1981 gttccgttcg cgtagccgta tttacccaag gtgcaaacgc tgaagctgct aaagctgcag
     2041 gcgcagaact ggtaggtatg gaagatctgg ctgaccagat caagaaaggc gaaatgaact
     2101 ttgacgttgt tattgcttct ccggatgcaa tgcgcgttgt tggccagctg ggccaggttc
     2161 tgggtccgcg cggcctgatg ccaaacccga aagtgggtac tgtaacaccg aacgttgctg
     2221 aagcggttaa aaacgctaaa gctggccagg ttcgttaccg taacgacaaa aacggcatca
     2281 tccacaccac catcggtaaa gtggactttg acgctgacaa actgaaagaa aacctggaag
     2341 ctctgctggt tgcgctgaaa aaagcaaaac cgactcaggc gaaaggcgtg tacatcaaga
     2401 aagttagcat ctccaccacc atgggtgcag gtgttgcagt tgaccaggct ggcctgagcg
     2461 cttctgtaaa ctaatgcctt tacgtgggcg gtgattttgt ctacaatctt acccccacgt
     2521 ataatgctta atgcagacgt atatccgaga tattcgggtt gtggcaaggc ggcaactgag
     2581 tgagtcgcca agagcatagc taactatgtg actggtgcga atgaaggaag ccaacgccgc
     2641 cacaagttga atagcgacgg atagaaaaga tttgttcgtt ggagcttggc ctatccaggc
     2701 ctccgtcgaa gaccgcagga gtttcgcaag aaacttaatc ccctgcgtag acggtgacag
     2761 aacgctaaga ttattctttt atattctggc ttgtttctgc tcaccgtaat taagacgctc
     2821 tctccgtttg gaggagtgaa gtgagttcca gagattttct ctggcaaaca tccaggagca
     2881 aagctaatgg ctttaaatct tcaagacaaa caagcgattg ttgctgaagt cagcgaagta
     2941 gccaaaggcg cgctgtctgc agtagttgcg gattcccgtg gcgtaactgt agataaaatg
     3001 actgaactgc gtaaagcagg tcgcgaagct ggcgtataca tgcgtgttgt tcgtaacacc
     3061 ctgctgcgcc gtgctgttga aggtactccg ttcgagtgcc tgaaagacgc gtttgttggt
     3121 ccgaccctga ttgcatactc tatggaacac ccgggcgctg ctgctcgtct gttcaaagag
     3181 ttcgcgaaag cgaatgcaaa atttgaggtc aaagccgctg cctttgaagg tgagctgatc
     3241 ccggcgtctc agatcgaccg cctggcaact ctgccgacct acgaagaagc aattgcacgc
     3301 ctgatggcaa ccatgaaaga agcttcggct ggcaaactgg ttcgtactct ggctgctgta
     3361 cgcgatgcga aagaagctgc ttaatcgcag ttatcttttt aacgcattcg cttacgtata
     3421 aacttattct gatattcagg aacaatttaa atgtctatca ctaaagatca aatcattgaa
     3481 gcagttgcag ctatgtctgt aatggacgtt gtagaactga tctctgcaat ggaagaaaaa
     3541 ttcggtgttt ccgctgctgc tgctgtagct gtagctgctg gcccggttga agctgctgaa
     3601 gaaaaaactg aattcgacgt aattctgaaa gctgctggcg ctaacaaagt tgctgttatc
     3661 aaagcagtac gtggcgcaac tggcctgggt ctgaaagaag ctaaagacct ggtagaatct
     3721 gcaccggctg ctctgaaaga aggcgtgagc aaagacgacg cagaagcact gaaaaaagct
     3781 ctggaagaag ctggcgctga agttgaagtt aaataagcca acccttccgg ttgcagcctg
     3841 agaaatcagg ctgatggctg gtgacttttt agtcaccagc ctttttgcgc tgtaaggcgc
     3901 cagtagcgtt tcacactgtt tgactactgc tgtgcctttc aatgcttgtt tctatcgacg
     3961 acttaatata ctgcgacagg acgtccgttc tgtgtaaatc gcaatgaaat ggtttaagcg
     4021 tgatagcaac aggcattgcg gaaagtgttc cattttccgg tcaacaaaat agtgttgcac
     4081 aaactgtccg ctcaatggac agatgggtcg acttgtcagc gagctgagga accctatggt
     4141 ttactcctat accgagaaaa aacgtattcg taaggatttt ggtaaacgtc cacaagttct
     4201 ggatgtacct tatctccttt ctatccagct tgactcgttt cagaaattta tcgagcaaga
     4261 tcctgaaggg cagtatggtc tggaagcagc tttccgttcc gtattcccga ttcagagcta
     4321 cagcggtaat tccgagctgc aatacgtcag ctaccgcctt ggcgaaccgg tgtttgacgt
     4381 ccaggaatgt caaatccgtg gcgtgaccta ttccgcaccg ctgcgcgtta aactgcgtct
     4441 ggtgatctat gagcgcgaag cgccggaagg caccgtaaaa gacattaaag aacaagaagt
     4501 ctacatgggc gaaattccgc tcatgacaga caacggtacc tttgttatca acggtactga
     4561 gcgtgttatc gtttcccagc tgcaccgtag tccgggcgtc ttctttgact ccgacaaagg
     4621 taaaacccac tcttcgggta aagtgctgta taacgcgcgc atcatccctt accgtggttc
     4681 ctggctggac ttcgaattcg atccgaagga caacctgttc gtacgtatcg accgtcgccg
     4741 taaactgcct gcgaccatca ttctgcgcgc cctgaactac accacagagc agatcctcga
     4801 cctgttcttt gaaaaagtta tctttgaaat ccgtgataac aagctgcaga tggaactggt
     4861 gccggaacgc ctgcgtggtg aaaccgcatc ttttgacatc gaagctaacg gtaaagtgta
     4921 cgtagaaaaa ggccgccgta tcactgcgcg ccacattcgc cagctggaaa aagacgacgt
     4981 caaacttatc gaagtcccgg ttgagtacat cgcaggtaaa gtggttgcta aagactatat
     5041 tgatgagtct accggcgagc tgatctgcgc agcgaacatg gagctgagcc tggatctgct
     5101 ggctaagctg agccagtctg gtcacaagcg tatcgaaacg ctgttcacca acgatctgga
     5161 tcacggccca tatatctctg aaaccttacg tgtcgaccca actaacgacc gtctgagcgc
     5221 actggtagaa atctaccgca tgatgcgccc tggcgagccg ccgactcgtg aagcagctga
     5281 aagcctgttc gagaacctgt tcttctccga agaccgttat gacttgtctg cggttggtcg
     5341 tatgaagttc aaccgttctc tgctgcgcga agaaatcgaa ggttccggta tcctgagcaa
     5401 agacgacatc attgatgtta tgaaaaagct catcgatatc cgtaacggta aaggcgaagt
     5461 cgatgatatc gaccacctcg gcaaccgtcg tatccgttcc gttggcgaaa tggcggaaaa
     5521 ccagttccgc gttggcttgg tacgtgtaga gcgtgcggtg aaagagcgtc tgtctctggg
     5581 cgatctggat accctgatgc ctcaggatat gatcaacgcc aagccgattt ccgcagcagt
     5641 gaaagagttc ttcggttcca gccagctgtc tcagtttatg gaccagaaca acccgctgtc
     5701 tgagattacg cacaaacgtc gtatctccgc actcggccca ggcggtctga cccgtgaacg
     5761 tgcaggcttc gaagttcgag acgtacaccc gactcactac ggtcgcgtat gtccaatcga
     5821 aacccctgaa ggtccgaaca tcggtctgat caactctctg tccgtgtacg cacagactaa
     5881 cgaatacggc ttccttgaga ctccgtatcg taaagtgacc gacggtgttg taactgacga
     5941 aattcactac ctgtctgcta tcgaagaagg caactacgtt atcgcccagg cgaactccaa
     6001 cttggatgaa gaaggccact tcgtagaaga cctggtaact tgccgtagca aaggcgaatc
     6061 cagcttgttc agccgcgacc aggttgacta catggacgta tccacccagc aggtggtatc
     6121 cgtcggtgcg tccctgatcc cgttcctgga acacgatgac gccaaccgtg cattgatggg
     6181 tgcgaacatg caacgtcagg ccgttccgac tctgcgcgct gataagccgc tggttggtac
     6241 tggtatggaa cgtgctgttg ccgttgactc cggtgtaact gcggtagcta aacgtggtgg
     6301 tgtcgttcag tacgtggatg cttcccgtat cgttatcaaa gttaacgaag acgagatgta
     6361 tccgggtgaa gcaggtatcg acatctacaa cctgaccaaa tacacccgtt ctaaccagaa
     6421 cacctgtatc aaccagatgc cgtgtgtgtc tctgggtgaa ccggttgaac gtggcgacgt
     6481 gctggcagac ggtccgtcca ccgacctcgg tgaactggcg cttggtcaga acatgcgcgt
     6541 agcgttcatg ccgtggaatg gttacaactt cgaagactcc atcctcgtat ccgagcgtgt
     6601 tgttcaggaa gaccgtttca ccaccatcca cattcaggaa ctggcgtgtg tgtcccgtga
     6661 caccaagctg gggccggaag agatcaccgc tgacatcccg aacgtgggtg aagctgcgct
     6721 ctccaaacta gatgaatccg gtatcgttta cattggtgcg gaagtgaccg gtggcgacat
     6781 tctggttggt aaggtaacgc cgaaaggtga aactcagctg accccagaag aaaaactgct
     6841 gcgtgcgatc ttcggtgaga aagcctctga cgttaaagac tcttctctgc gcgtaccaaa
     6901 cggtgtatcc ggtacggtta tcgacgttca ggtctttact cgcgatggcg tagaaaaaga
     6961 caaacgtgcg ctggaaatcg aagaaatgca gctcaaacag gcgaagaaag acctgtctga
     7021 agaactgcag atcctcgaag cgggtctgtt cagccgtatc cgtgctgtgc tggtagccgg
     7081 tggcgttgaa gctgagaagc tcgacaaact gccgcgcgat cgctggctgg agctgggcct
     7141 gacagacgaa gagaaacaaa atcagctgga acagctggct gagcagtatg acgaactgaa
     7201 acacgagttc gagaagaaac tcgaagcgaa acgccgcaaa atcacccagg gcgacgatct
     7261 ggcaccgggc gtgctgaaga ttgttaaggt atatctggcg gttaaacgcc gtatccagcc
     7321 tggtgacaag atggcaggtc gtcacggtaa caagggtgta atttctaaga tcaacccgat
     7381 cgaagatatg ccttacgatg aaaacggtac gccggtagac atcgtactga acccgctggg
     7441 cgtaccgtct cgtatgaaca tcggtcagat cctcgaaacc cacctgggta tggctgcgaa
     7501 aggtatcggc gacaagatca acgccatgct gaaacagcag caagaagtcg cgaaactgcg
     7561 cgaattcatc cagcgtgcgt acgatctggg cgctgacgtt cgtcagaaag ttgacctgag
     7621 taccttcagc gatgaagaag ttatgcgtct ggctgaaaac ctgcgcaaag gtatgccaat
     7681 cgcaacgccg gtgttcgacg gtgcgaaaga agcagaaatt aaagagctgc tgaaacttgg
     7741 cgacctgccg acttccggtc agatccgcct gtacgatggt cgcactggtg aacagttcga
     7801 gcgtccggta accgttggtt acatgtacat gctgaaactg aaccacctgg tcgacgacaa
     7861 gatgcacgcg cgttccaccg gttcttacag cctggttact cagcagccgc tgggtggtaa
     7921 ggcacagttc ggtggtcagc gtttcgggga gatggaagtg tgggcgctgg aagcatacgg
     7981 cgcagcatac accctgcagg aaatgctcac cgttaagtct gatgacgtga acggtcgtac
     8041 caagatgtat aaaaacatcg tggacggcaa ccatcagatg gagccgggca tgccagaatc
     8101 cttcaacgta ttgttgaaag agattcgttc gctgggtatc aacatcgaac tggaagacga
     8161 gtaattctcg ctcaaacagg tcactgctgt cgggttaaaa cccggcagcg gattgtgcta
     8221 actccgacgg gagcaaatcc gtgaaagatt tattaaagtt tctgaaagcg cagactaaaa
     8281 ccgaagagtt tgatgcgatc aaaattgctc tggcttcgcc agacatgatc cgttcatggt
     8341 ctttcggtga agttaaaaag ccggaaacca tcaactaccg tacgttcaaa ccagaacgtg
     8401 acggcctttt ctgcgcccgt atctttgggc cggtaaaaga ttacgagtgc ctgtgcggta
     8461 agtacaagcg cctgaaacac cgtggcgtca tctgtgagaa gtgcggcgtt gaagtgaccc
     8521 agactaaagt acgccgtgag cgtatgggcc acatcgaact ggcttccccg actgcgcaca
     8581 tctggttcct gaaatcgctg ccgtcccgta tcggtctgct gctcgatatg ccgctgcgcg
     8641 atatcgaacg cgtactgtac tttgaatcct atgtggttat cgaaggcggt atgaccaacc
     8701 tggaacgtca gcagatgctg actgaagagc agtatctgga cgcgctggaa gagttcggtg
     8761 acgaattcga cgcgaagatg ggtgcggaag caatccaggc tctgctgaag agcatggatc
     8821 tggagcaaga gtgcgaacag ctgcgcgaag agctgaacga aaccaactcc gaaaccaagc
     8881 gtaaaaagct gaccaagcgt atcaaactgc tggaagcgtt cgttcagtct ggtaacaaac
     8941 cagagtggat gatcctgacc gttctgccgg tactgccgcc agatctgcgt ccgctggttc
     9001 cgctggatgg tggtcgtttc gcgacttctg acctgaacga tctgtatcgt cgcgtcatta
     9061 accgtaacaa ccgtctgaaa cgtctgctgg atctggctgc gccggacatc atcgtacgta
     9121 acgaaaaacg tatgctgcag gaagcggtag acgccctgct ggataacggt cgtcgcggtc
     9181 gtgcgatcac cggttctaac aagcgtcctc tgaaatcttt ggccgacatg atcaaaggta
     9241 aacagggtcg tttccgtcag aacctgctcg gtaagcgtgt tgactactcc ggtcgttctg
     9301 taatcaccgt aggtccatac ctgcgtctgc atcagtgcgg tctgccgaag aaaatggcgc
     9361 tggagctgtt caaaccgttc atctacggca agctggaact gcgtggtctt gctaccacca
     9421 ttaaagctgc gaagaaaatg gttgagcgcg aagaagctgt cgtttgggat atcctggacg
     9481 aagttatccg cgaacacccg gtactgctga accgtgcacc gactctgcac cgtctgggta
     9541 tccaggcatt tgaaccggta ctgatcgaag gtaaagctat ccagctgcac ccgctggttt
     9601 gtgcggcata taacgccgac ttcgatggtg accagatggc tgttcacgta ccgctgacgc
     9661 tggaagccca gctggaagcg cgtgcgctga tgatgtctac caacaacatc ctgtccccgg
     9721 cgaacggcga accaatcatc gttccgtctc aggacgttgt tctgggtctg tactatatga
     9781 cccgtgactg tgttaacgcc aaaggcgaag gcatggtgct gactggcccg aaagaagcag
     9841 aacgtctgta tcgctctggt ctggcttctc tgcatgcgcg cgttaaagtg cgtatcaccg
     9901 agtatgaaaa agatgcaaac ggtgaattag tagcgaaaac cagcctgaaa gacacgactg
     9961 ttggccgtgc cattctgtgg atgattgtac cgaaaggtct gccttactcc atcgtcaacc
    10021 aggcgctggg taaaaaagca atctccaaaa tgctgaacac ctgctaccgc attctcggtc
    10081 tgaaaccgac cgttattttt gcggaccaga tcatgtacac cggcttcgcc tatgcagcgc
    10141 gttctggtgc atctgttggc atcgatgaca tggtcatccc ggagaagaaa cacgaaatca
    10201 tctccgaggc agaagcagaa gttgctgaaa ttcaggagca gttccagtct ggtctggtaa
    10261 ctgcgggcga acgctacaac aaagttatcg atatctgggc tgcggcgaac gatcgtgtat
    10321 ccaaagcgat gatggataac ctgcaaactg aaaccgtgat taaccgtgac ggtcaggaag
    10381 agaagcaggt ttccttcaac agcatctaca tgatggccga ctccggtgcg cgtggttctg
    10441 cggcacagat tcgtcagctt gctggtatgc gtggtctgat ggcgaagccg gatggctcca
    10501 tcatcgaaac gccaatcacc gcgaacttcc gtgaaggtct gaacgtactc cagtacttca
    10561 tctccaccca cggtgctcgt aaaggtctgg cggataccgc actgaaaact gcaaactccg
    10621 gttacctgac tcgtcgtctg gttgacgtgg cgcaggacct ggtggttacc gaagacgatt
    10681 gtggtaccca tgaaggtatc atgatgactc cggttatcga aggtggtgat gttaaagagc
    10741 cgctgcgcga tcgcgtactg ggtcgtgtaa ctgctgaaga cgttctgaag ccgggtactg
    10801 ctgatatcct cgttccgcgc aacacgctgc tgcacgaaca gtggtgtgac ctgctggaag
    10861 agaactctgt cgacgcggtt aaagtacgtt ctgttgtatc ttgtgacacc gactttggtg
    10921 tatgtgcgca ctgctacggt cgtgacctgg cgcgtggcca catcatcaac aagggtgaag
    10981 caatcggtgt tatcgcggca cagtccatcg gtgaaccggg tacacagctg accatgcgta
    11041 cgttccacat cggtggtgcg gcatctcgtg cggctgctga atccagcatc caggtgaaaa
    11101 acaaaggtag catcaagctc agcaacgtga agtcggttgt gaactccagc ggtaaactgg
    11161 ttatcacttc ccgtaatact gaactgaaac tgatcgacga attcggtcgt accaaagaaa
    11221 gctacaaagt accttacggt gcggtactgg cgaaaggcga tggcgaacag gttgctggcg
    11281 gcgaaaccgt tgcaaactgg gacccgcaca ccatgccggt tatcaccgaa gtaagcggtt
    11341 ttgtacgctt tactgacatg atcgacggtc agaccattac tcgtcagaca gacgaattga
    11401 ccggtctgtc ttcgctggtg gttctggatt ccgcagaacg taccgcaggt ggtaaagatc
    11461 tgcgtccggc actgaaaatc gttgatgctc agggtaacga cgttctgatt ccgggtaccg
    11521 atatgcctgc gcagtacttc ctgccgggta aagcgattgt tcagctggaa gatggcgtac
    11581 agatcagctc tggtgacacc ctggcgcgta ttccgcagga atccggcggt accaaggaca
    11641 tcaccggtgg tctgccgcgc gttgcggacc tgttcgaagc acgtcgtccg aaagagccgg
    11701 caatcctggc tgaaatcagc ggtatcgttt ccttcggtaa agaaaccaaa ggtaaacgtc
    11761 gtcttgttat caccccggta gacggtagcg atccgtacga agagatgatt ccgaaatggc
    11821 gtcagctcaa cgtgttcgaa ggtgaacgtg tagaacgtgg tgacgtaatt tccgacggtc
    11881 cggaagcgcc gcacgacatt ctgcgtctgc gtggtgttca tgctgttact cgttacatcg
    11941 ttaacgaagt acaggacgta taccgtctgc agggcgttaa gattaacgat aaacacatcg
    12001 aagttatcgt tcgtcagatg ctgcgtaaag ctaccatcgt taacgcgggc agctccgact
    12061 tcctggaagg cgaacaggtt gaatactctc gcgtcaagat cgcaaaccgc gaactggaag
    12121 cgaacggcaa agtgggtgca acttactccc gcgatctgct gggtatcacc aaagcgtctc
    12181 tggcaaccga gtccttcatc tccgcggcat cgttccagga gaccactcgc gtgctgaccg
    12241 aagcagccgt tgcgggcaaa cgcgacgaac tgcgcggcct gaaagagaac gttatcgtgg
    12301 gtcgtctgat cccggcaggt accggttacg cgtaccacca ggatcgtatg cgtcgccgtg
    12361 ctgcgggtga agctccggct gcaccgcagg tgactgcaga agacgcatct gccagcctgg
    12421 cagaactgct gaacgcaggt ctgggcggtt ctgataacga gtaatcgtta atccgcaaat
    12481 aacgtaaaaa cccgcttcgg cgggtttttt tatgggggga gtttagggaa agagcatttg
    12541 tcagaatatt taaggaattt ctgaatactc ataatcaatg tagagatgac taatatcctg
    12601 aaactgactg aactaattga gtcaaactcg gcaaggattc gatactattc ctgtgtaact
    12661 ttcttaagga acgagaatga aacaggaagt ggaaaagtgg cgaccttttg gacatccgga
    12721 tggtgatatt cgtgatttat catttcttga tgctcatcag gctgtctacg ttcagcatca
    12781 tgagggcaaa gagcctttag agtatcgctt ttgggttacc tactctcttc actgcttcac
    12841 aaaagattat gaacatcaga cgaacgaaga aaaacaatcg ttaatgtacc acgcgcctaa
    12901 agaatctcgt cccttctgcc agcaccgtta taacttagcg cgcacacact taaaaagaac
    12961 tattttggcg ctgccagaaa gcaacgttat tcatgccggg tatggtagct atgccgtgat
    13021 tgaggtggac ttagacggag gagataaggc attttacttt gttgcgttca gggctttcag
    13081 ggaaaagaaa aaactccgtt tgcatgtaac tagcgcttat cccatttctg aaaaacagaa
    13141 aggtaaatca gtgaaatttt tcaccattgc ctacaactta ttgagaaata agcagcttcc
    13201 tcagccctca aaataacaaa acccacctta aggtgggttt cgccagagaa ttatctctgg
    13261 tattcagaac gccattaccg gactttgcct tgaccttgcg ataatcgcag gttgcgggat
    13321 gtctgaattt cttcagtctg ctgcatcctg gaagatgaga acatgtgttc ttattttcgt
    13381 ctctatcata gttgagtatt tactctctta caatcagatc tctttcattg ctcaacaggc
    13441 gatggcttca gactttgcat tacggaattt ttaagaaagg cagggcgaaa cgaggaagaa
    13501 gcttttcgcc ctggacaaca ttcctgctga cggcactacc ataaaaaaac acaacgcttt
    13561 caacaacctt gcgtttttca tcttccgtag cggctcggaa taaatttacg ttcttacatt
    13621 ccgtctcata gtctttgcga ggcccgtccc agatagctgt cccagtcttt ccataccggc
    13681 tgcaaaccct gagcggttaa cgcggcagca accgcttccg gtctgcgatc gtcgtgcggt
    13741 gagaactgtt ccaactcggg gtgattatcg gcatagccac ctggctgcgt tttcgagaag
    13801 gcgctgacgt tattgatcgc cagcggaata acgcgatcgc gaaaccacgg tgattcccgc
    13861 gtggagagtg acagttcaat ctccggtgca agcagtcgga aggcgcagat ggtttgcact
    13921 aactggcgtt catccataat cgacgcaggc tcaatgccgc cagtacacgg gcgcaggcgc
    13981 ggaaaggaga cagagtaacg gctttgccag taatgctgtt gcagccatag caaatgttcg
    14041 gcaaccatat agcagtcaac gcgccagttg tcggaaaggc caattagcgc gccgaggcct
    14101 atcttatcaa tccccgcacg ccccagccga tccggcgttt ccagccgcca gaagaagtcc
    14161 tgttttttgc ctttcagatg atggcgggca taagtcgcct cgtgatatgt ctcctgataa
    14221 accatcacgc catccagacc aagctgcttt aactcggcgt attccgtctt cgccagcggt
    14281 tgcacttcca tttgtagtga agagaactgt tcacgcaggg cggggagatg acgacgaaag
    14341 taatccatcc ccactttcgc ctgatgttca ccagtgacta atagcagatg ttcaaagccc
    14401 atctcccgta tagcggcgct ttccctggca atatccgctt catccagcgt tttgcgcttg
    14461 atgcgattac tcatggaaaa tccgcagtac gtgcagtcgt tagcgcaaag attggaaaga
    14521 taaagcggga cgtagaaact aactgtgttg ccaaatcgct gacgggtcag acgctgcgcc
    14581 cgttgggcca gttgttccag atagccactg gcggctggcg ataacagcgc catcatatcg
    14641 tcgcgggtga attgcgaggc atttagcgcc cgctctacgt caacagccgt tttgccgttg
    14701 atacgcaggc ggatgtcatc ccagtccagt tgtcgccagc gatcgctgaa ggttttcatg
    14761 ccgatgcctc cagaaatcca gtcagcgggc tggtggcatg agcaaaatga ctgcggctgc
    14821 ccggtccgga ctgacgtgcc agcaggcctg cttctaccgc cagacgaaat gccttcgcca
    14881 tgttgacggg atcgtccgct acggcaatcg ccgtattcac taacaccgcg tcggccccca
    14941 tttccagggc ctgcgctgca tggctgggaa cgccgatgcc agcatcgaca accaccggaa
    15001 ctgtggcctg ctggataata atctccagca tggcacgggt ttccagtccc tgattcgagc
    15061 caatcggcgc gccgagcggc atcaccgctg cacagccgac ttcttccaga cgtttacaca
    15121 ataccggatc ggccccgcag taaggcagca cgacaaatcc ctgttgtacc agcatttcgg
    15181 cggctttcag agtttcgatg ggatcgggca acagccagcg ggcgtcaggg tgaatctcta
    15241 attttaacca gtttgtgcct aacgcttcac gtgccagatg ggcggcgaaa atggcttctt
    15301 ccgctgtttt cgccccggaa gtatttggca gcagggtcac acccgccgcg ataagcggtt
    15361 cgaggatagc gtcgttgtgc tggcgcaagt cgacacgttt catcgccagt gtcaccagct
    15421 ggctgccgga agcgcggatc gcctccacca tcagttgtga agaagcgaat ttccctgtgc
    15481 cggtaaacag atgtgaatca aacgttttgt ccgcaatacg taacatctca accccctgca
    15541 ataacctgaa aaagcaggat ctggtcgcca tcctgcagga tatgttgcgt ccactgctca
    15601 cgcgggacga tttgctgatt aatcgccaga gccgcgcccg cttgtcgttg gtcgagttgc
    15661 tccagtagtt cgtgaacagt ttgcccggtg gcgcactgca tcggttgatc gttaaacagg
    15721 atctgcattg cttcctccgc ataccgggca accactggcg cggcgcaacg ccaggctgcg
    15781 ccactggctc gatttaccgt cgaacagtcg gagttctccc gcaggtgtct ctataccgct
    15841 taataactta atggcttcca gtgcctgcaa agtgcccata accccgacca ccgggccaac
    15901 cacgcccgcc gtgcggcagt tgcgttctgg ctcctggtta tctggccaca ggcagcggta
    15961 acacccctgc tcccagggcg gcgtcagtac catcaactga ccgccaaatc cgaccgcgct
    16021 ggcggtgata agcggcgtgt tgagtaccac gcaggcggca ttaatctcct ggcgagtcgc
    16081 catattgtcg gtacagtcga gcaccacatc ggcccgtgca accgcatctt ttaacgcctc
    16141 acccgttaac cgttgttgta atgctgtcag ttgaatgtcg ggattcaact gtgtcagtcg
    16201 ctgttggctg acctgcgatt tcgggctgtt gatatcttca gtggtaaaga gaatttgtcg
    16261 ttacagattg cttaaatgca catcgtcgtc atctgccagt accagcgtcc cgacgccagc
    16321 gcccgccagg tatagcgcag caggtgtacc cagcccgcca agaccgataa tcagcacctg
    16381 gctgtcgagc agtttttgct gcccgtcaag agcgatatca tcgagcagga tttggcggct
    16441 ataacgcata aaatcacggt cattcatcgc caactcctgc aatttccagc aactgtgccg
    16501 ttgccaaacg ccagtctgcg gcttgagtaa tggcgctgat gacggcgata ctgccgacac
    16561 ccgttgctat caccgcaggc gcgcgtgcca gactgatacc gccaatcgcc acggtgggat
    16621 aatccgccag tcgctcaaca tgccgtgcca gctgttccag cccctgcggt gcagaaggca
    16681 tctgtttggt ttgcgtcggg aacacatgtc ccagcgcgat ataagagggg cgtgctgcca
    16741 gcgcgacgtc gatttccata tcgtcatgtg tcgaaacacc cagccgcagg cctgccgcgc
    16801 ggatggcatt gagatcggtg gcttgcaaat cttcctgccc caaatggacg ccatacgcct
    16861 gatgcttgat cgcgagccgc cagtaatcgt tgataaacaa tcgcgcgtta tagcggcggc
    16921 ccagcgcaat tgccgccacg acatcggctt ccacctcttc atcgcgccga tctttgatgc
    16981 gtagctggag agtacgtacg cctgcatcca acagacgttc gatccactgt acgctgtcca
    17041 ccactgggta cagtcctaaa cgaaaaggta caggaggaaa atcaggctga tacatcacgc
    17101 ttcctcctta cgcaggtaga tttctccgcc tctggcacgg aagttctccg acatatccgc
    17161 cattcccatt tcaatagttt gcgtggcggc gtaatcacgc acttcctggc tgattttcat
    17221 cgagcagaat ttcggcccac acatggagca aaaatgggcg actttacctg actcttgcgg
    17281 cagggtttca tcgtgataag cgcgggcggt aaacgggtcg agggccagat taaactggtc
    17341 ttcccagcga aattcgaagc gggctttcga catggcgtta tcgcgaattt gcgcgcccgg
    17401 atgccctttc gccaggtcag cggcgtgggc ggcaatctta taggtgataa gcccctgctt
    17461 aacatcttct ttattaggca gacccagatg ctcttttggt gttacgtaac agagcatcgc
    17521 gcagccaaac cagccaatca tcgccgcacc aatccccgac gtgaagtggt catagcccgg
    17581 cgcaatatcg gtagttagcg gccccagagt gtaaaacggc gcttcgtggc agtgctctaa
    17641 ctcctcggtc atattgcggc ggatcatctg catcggcacg tggcctgggc cttcaatcat
    17701 cacctgcacg tcatattccc aggcaatttt ggtcagttcg cccagcgtat gcagctcggc
    17761 aaactgcgct tcatcgttgg cgtcctgaat agaaccgggg cgcagaccgt cgcccagcga
    17821 cagcgaaacg tcataagcgg cacagatttc acaaatttcg cggaagtgtt gatagaggaa
    17881 attttcctga tgatgggaga ggcaccattt cgccataatc gaaccgccgc gagagacgat
    17941 accggtcagg cgtttcgcgg tcatcggcac atagcgcaac agtacgcccg catggatagt
    18001 gaagtaatcc acaccttgct cggcctgttc cagcagcgtg tcgcggaacg cttcccaggt
    18061 aagatcttcg gcgatcccgt taaccttctc cagcgcctgg tagatcggca ctgtaccgat
    18121 cggcaccgga ctgttacgca aaatccactc gcgggtttcg tgaatatagc gaccggtgga
    18181 gagatccatc accgtatccg ctccccagcg cgtggaccat accagctttt ccacttcttc
    18241 ttcgatggaa gaggtgaccg ccgagttgcc gatattggcg ttaactttta ccaggaagtt
    18301 acggccaata atcatcggct ccgattccgg atgattaatg ttggccggga taatcgcacg
    18361 tccggcagca acttcatcac ggacaaattc cgcagtgata ttttccggca gacgtgcgcc
    18421 aaagctcatt cccggatgct ggtggcgtaa aacttcgcta cgtatgcgct cgcggcccat
    18481 attctcgcgg atggcgatga attccatttc cggagtgatg atgccctggc gggcgtagtg
    18541 cagttgggtg acacggcgtc ctgctttggc gcgttttggt gttagtacgc cgctaaaacg
    18601 cagttcgtcg aggccatcat ctgccagccg cgctttagtg taatcggaac tgcgcacggt
    18661 aagttcttcg gtatcgccgc gcgcatcgat ccacggctgg cgtagttttg ccagcccttg
    18721 ctgcacgtta atggcgatct gcggatcgcc atatgggccg gaggtgtcgt agaccggaat
    18781 cgcttcgttt tcttcaaact gcggctgttc tttgctgccg ccgattagcg tcgggctaag
    18841 ctggatctca cgcatcggca cgcgaacgcc agagtgtgtg ccagtgaggt aaatgcgttt
    18901 tgagttggga aaggcggtgc cttccagggt gtcgataaaa tgttgggccc gggcgcgttg
    18961 ttcgcggcgg gtcagttttg ttgcagacat agctcattcc aaaaagttaa ggacgtggct
    19021 tgtcagacga cggatgaagc aagagacgat cgccgcaaag gcgatgcgat agcagattaa
    19081 ctcttgttcc cttcgcaggt attagcctga tcaggttccg cggatcccga ataaacggtc
    19141 tcagccagtt aaggcactcc gacaagaaat tagcccccac aaacggggcg ttgaatgtaa
    19201 attacgcgtt aacagcgcag aactcaagca ggatgtttga cgcgggcggc gtcaagcacc
    19261 agcagaatca gcttatcttc cagcacaaag cgcgcttcca gcgcctcgcc gatgtcagat
    19321 aaaacctttt gaaactcaag gtaattatca tgatcgatgg cggtttccag gctggaatcg
    19381 tagtaatcca taatctgttg ggtattggct tcgagttgcg gccaaatctt cgcggcgcgc
    19441 gcgagttgcc cgttgccttc cagcttatga agaatgcgct cataaatact gaaatgtccg
    19501 gcagacaagt aatcgaccag gctctgacaa aaatcatcaa gggctttttc gtttagcctc
    19561 atgtacgatt ctttgccagg cttaatgcca accagattgt agtaagccac gagcagatgc
    19621 ttacgtacat gtagccagcg atcaaccagt ttgttacttc ctctgacgcg ctccgtcagg
    19681 ttatcgagct ggttaagcat gattgactcc gcaagtttgt attcaaaaac tgctcagtga
    19741 gaaatgtaaa aaccatgtta aacatgccag tgatgcaaag gtagtgcaag agctatggat
    19801 cgtataattg aaaaattaga tcacggctgg tgggtcgtca gccatgaaca aaaattatgg
    19861 ttaccgaagg gagaattgcc atatggcgaa gcggcaaatt tcgatcttgt gggtcagcgc
    19921 gcactacaaa tcggcgaatg gcagggggaa cctgtttggt tagtacaaca gcagcggcgt
    19981 cacgatatgg ggtcggtacg tcaggtcatt gatctcgatg ttgtgctgtt tcaactggcc
    20041 ggacgaggcg tacaactggc ggagttttac cgatcgcata aatactgtgg ttactgcggg
    20101 catgaaatgt atccgagcaa aaccgaatgg gcgatgctgt gcagccattg ccgtgagcgt
    20161 tactacccgc aaatcgcccc ctgcattatt gttgccatcc gtcgcgatga ttcgatcctc
    20221 ctcgcccagc atacccgcca tcgtaacggt gtccatacag tacttgccgg attcgtcgaa
    20281 gtgggcgaaa ccctcgagca ggcagtcgcg cgggaagtga tggaagagag cggaattaaa
    20341 gttaaaaact tgcgttacgt gacttctcag ccgtggccgt ttcctcagtc tttaatgacc
    20401 gcgtttatgg cggaatatga cagcggcgac atcgtgatcg acccgaaaga attgctcgag
    20461 gcgaactggt atcgctatga cgatttgccg ttactcccgc cgcccggcac cgtagcgcgc
    20521 cgtctgatag aagatactgt ggcgatgtgt cgggcagagt atgagtgatg atacactgac
    20581 cgcctgacgc actaaggaac agccaaaatg accgaactta aaaacgatcg ttatctgcgg
    20641 gcgctgctgc gccagcccgt tgatgtcact ccagtatgga tgatgcgcca ggcgggtcgc
    20701 tatctaccgg aatataaagt cacgcgcgcc caggcgggcg attttatgtc gctgtgcaaa
    20761 aacgccgagc tggcgtgcga agtgactttg caaccgctgc gtcgctaccc gctggatgcg
    20821 gcgatcctct tttccgatat cctcaccgtg ccggacgcga tggggttagg gctctatttt
    20881 gaagccggag aaggtccgcg ttttacctcg ccagtcacct gcaaagccga cgtcgataag
    20941 ctgccaattc cggacccgga agatgagctg ggttacgtga tgaacgcggt gcgtaccatt
    21001 cgtcgcgaac tgaaaggcga agtgccgctg attggttttt ccggcagccc gtggacgctg
    21061 gcaacctaca tggtggaagg cggcagcagc aaagccttca ccgtgatcaa aaaaatgatg
    21121 tatgccgatc cgcaggcgct gcacgctcta ctcgataaac tggcgaaaag cgtcactttg
    21181 tatctgaatg cgcagattaa agccggtgct caggcagtga tgattttcga cacctggggc
    21241 ggtgtgctta ccgggcgcga ttatcaacag ttctcgctct attacatgca taaaattgtt
    21301 gatggtttac tgcgtgaaaa cgacggtcgc cgcgtaccgg tcacgctgtt taccaaaggc
    21361 ggcggacagt ggctggaagc gatggcagaa accggttgcg atgcgttggg cctcgactgg
    21421 acaacggata tcgccgatgc gcgccgccgt gtgggcaata aagtcgcgtt gcagggtaat
    21481 atggatccgt cgatgctgta cgcgccgcct gcccgcattg aagaagaagt agcgactata
    21541 cttgcaggtt tcggtcacgg cgaaggtcat gtctttaacc ttggtcacgg cattcatcag
    21601 gatgtgccgc cagaacatgc tggcgtgttc gtggaggcag tgcatcgact gtctgaacaa
    21661 tatcaccgct aaggagtgat tatggatctc gcgtcattac gcgctcaaca aatcgaactg
    21721 gcttcttctg tgatccgcga ggatcgactc gataaagatc caccggatct gatcgccgga
    21781 gccgatgtcg ggtttgagca gggcggagaa gtgacgcgag cggcgatggt gctgctgaaa
    21841 tatccctcgc ttgagctggt ggagtataaa gttgcccgca tcgccaccac catgccttac
    21901 attccaggtt ttctttcctt ccgcgaatat cctgcgctgc tggcagcgtg ggagatgctg
    21961 tcgcaaaagc cggatttagt gtttgtcgat ggtcatggga tctcgcatcc tcgccgtctt
    22021 ggcgtcgcca gccattttgg tttgatggtg gatgtgccaa cgatcggcgt ggcgaaaaaa
    22081 cggctctgcg gtaaattcga accgctctcc agcgaaccgg gcgcgctggc cccactgatg
    22141 gataaaggcg agcagctggc ctgggtctgg cgcagcaaag cgcgctgtaa cccgttgttt
    22201 atcgctaccg gccatcgggt cagcgtggac agcgcgctgg cgtgggtaca acgctgcatg
    22261 aaaggctatc gtctgccgga gccaacgcgc tgggcggacg cggtggcctc ggaacgtccg
    22321 gcgttcgtgc gctatacagc aaatcagccc taattcaggt aaactgcgga taatttccgt
    22381 atttgagaac ttaacatgtt acaaaaccca attcatctgc gtctggagcg cctggaaagc
    22441 tggcagcatg tcactttcat ggcttgctta tgcgaacgca tgtaccccaa ttacgccatg
    22501 ttctgccagc aaaccggttt tggtgatggg caaatttacc gtcgtattct cgatctcatc
    22561 tgggaaacgc tgaccgttaa agacgcaaaa gtaaatttcg acagccaact ggagaaattt
    22621 gaagaagcga ttccttcagc cgacgatttc gatctgtacg gcgtttatcc ggcaatcgat
    22681 gcctgcgtgg tgttaagtga actggtccat tcgcgtttga gtggtgaaac gctcgaacac
    22741 gcggtggaag tgagtaagac ctccatcacg accgttgcga tgctggaaat gactcaggtt
    22801 ggtcgcgaaa tgagcgatga agagcttaaa gaaaacccag ctgtagagca agaatgggac
    22861 attcagtggg aaatattccg acttttagct gagtgcgaag aacgcgatat cgagctgata
    22921 aaaggcctta gggcagacct gcgtgaggcg ggtgagagca atattggtat aatttttcag
    22981 caataagacc agaaaacgtg atttaacgcc tgatttgtcg tacttggagt cttccctttc
    23041 gccccccgtc tggtctacat ttggggggcg aaaaaaagtg gctatcggtg cgtgtatgca
    23101 ggagagtgct attctggcat ttccgtcgca ctcgatgctt agcaagcgat aaacacattg
    23161 taaggataac ttatgaacaa gactcaactg attgatgtaa ttgcagagaa agcagaactg
    23221 tccaaaaccc aggctaaagc tgctctggag tccactctgg ctgcaattac tgagtctctg
    23281 aaagaaggcg atgctgtaca actggttggt ttcggtacct tcaaagtgaa ccaccgcgct
    23341 gagcgtactg gccgcaaccc gcagaccggt aaagaaatca aaattgccgc agctaacgta
    23401 ccggcatttg tttctggcaa ggcactgaaa gacgcagtta agtaagattg cgtggcagtg
    23461 aacagtttta acgaaggggt ggtttcaccc cttttgtctt tctggcgtcg atcattgatg
    23521 ctggctggcg ctctgcttct cactgcctgt agtcataact cttcacttcc tccctttacc
    23581 gccagtggat ttgctgaaga ccagggcgcg gtacgcatct ggcgaaaaga cagcggcgat
    23641 aatgtgcatc tgcttgccgt gtttagcccg tggcgcagtg gcgataccac gacgcgagag
    23701 tatcgctggc agggcgataa cctcacgctc atcaatatca atgtttacag caaaccgccg
    23761 gtgaatattc gcgcgcgttt tgacgatcgc ggtgatctga gctttatgca acgtgaatcc
    23821 gatggggaaa agcagcagct ttctaacgac caaatcgatt tataccgtta tcgtgctgat
    23881 cagatccgcc agattagcga tgccttacgt caggggagag tcgtgctgcg ccaggggcgc
    23941 tggcatgcga tggaacagac cgtgaccacc tgcgaagggc aaaccattaa acctgattta
    24001 gattcgcagg cgatagcgca tatcgagcgc cgccagagcc gctcttctgt tgatgtcagc
    24061 gtggcatggc tggaagcgcc cgaaggttcg caattactgt tagtggcaaa ctctgatttc
    24121 tgtcgctggc aacccaacga gaaaacgttc tgatttacca gtggcccata cccatatgac
    24181 cgccaccacc gcagccgccg tagcccatgc ccattccggc tccgcgcgga atacccgctt
    24241 cagccatcgc gatatctcgt ttcacccgta actcatctaa cgactgacgc aaattctcca
    24301 tctctttggc gaccgcgtta attttgctgc tatccggtgg gttcgcggct aacagggcat
    24361 tgtattcata acgcttcgtc accagttgct gttgcagtgc gctgctttga gcgtaaaagt
    24421 cattatggat tttctgccac gctgtctgtt gttcgctggt caaaggcgcg gcattttgct
    24481 gccacatacc gtgtccgccg tgagcaaatg cagatgtcga tcccatcgcc attgctgaaa
    24541 gcgccatcat taccagggca attttcgtgt tccgtttcat ggttaatcct ccagtggttg
    24601 tcttacttcg ggtattgcat cttccgtgcc aacgatgaaa cgctgatatg acgggtaatc
    24661 tggcatgata aacgagtaaa aatgactcgc ctgctgcggg tagcgagtca tttttactca
    24721 ttgaaactta agcctttgtg ttacagcgca gggtaagcgc tgataaaaga tggcatgatt
    24781 tctgctgtca gaaagggatg agcaggcaaa gaagaagatg cgttttatgc aacgttctaa
    24841 agactcctta gctaaatggt taagcgcgat cctccccgtg gtcattgttg ggctggtagg
    24901 gttgtttgcg gtgacggtga ttcgcgatta tgggcgcgag actgccgccg ccagacaaac
    24961 gctgctggaa aaaggcagtg tacttattcg cgctcttgaa tccggctcgc gcgtcggcat
    25021 ggggatgcgc atgcatcatg cgcagcagca ggccttactg gaagaaatgg ccgggcagcc
    25081 tggagtacgt tggtttgcgg tcacggatga acaaggaaca atcgtgatgc atagcaactc
    25141 cggcatggtg ggaaaacagc tttattcccc gcaggaaatg cagcagttac atccgggaga
    25201 tgaagaagcg tggcggcgga tcgatagcgc agacggtgag cctgttctgg aaatttatcg
    25261 ccagtttcaa ccgatgtttg ctgctggaat gcaccggatg cgccatatgc agcagtatgc
    25321 cgcgacacca caagcaattt tcatcgcttt cgacgccagt aatattgtga gtgccgaaga
    25381 tcgtgagcag agaaacaccc tgattatcct cttcgccctg gcgacggtct tgctggcaag
    25441 cgtattgtca ttcttctggt atcgccgcta tctgcgctcg cgccagcttc tacaagatga
    25501 aatgaagcgc aaagagaagc tggtggcact ggggcatctg gcggcaggcg ttgcccacga
    25561 aatccgtaat ccactttcct caattaaagg gctggcgaaa tactttgccg aacgcgcgcc
    25621 agcaggggga gaagcgcatc aactggcgca ggtgatggcg aaagaagccg accgtttaaa
    25681 ccgcgtggta agcgagttgc tggaactggt taagccaacg catctggctt tgcaggcggt
    25741 ggatctcaac acgctgatta accactcatt acagctggta agccaggatg caaacagccg
    25801 ggagatccag ttacgcttta ccgccaacga cacattaccg gaaattcagg ccgatccgga
    25861 caggctgact caggtcctgt tgaatctcta tctcaatgct attcaggcga ttgttcagca
    25921 tggcgtgatt agcgtgacgg tcagcgaaag cggcgcgggc gtgaaaatca gcgttaccga
    25981 cagcggtaag ggaattgcgg cagatcagct tgaagccatc ttcactccgt acttcaccac
    26041 caaagccgaa ggcaccggat tggggctggc ggtcgtgcat aatattgttg aacaacacgg
    26101 tggtacaatt caggtcgcaa gccaggaggg aaaaggctca acgttcaccc tctggcttcc
    26161 ggtcaatatt acgcgtaagg acccacaagg atgacgcacg ataatatcga tattctggtg
    26221 gtggatgatg acattagcca ctgcactatt ttgcaggctt tactgcgcgg ctggggctat
    26281 aacgtcgcgc tggcgaacag cgggcgacag gcgctggagc aggtgcggga acaggttttt
    26341 gatcttgtgc tttgcgatgt gcgaatggcg gagatggacg gcatcgccac gctgaaagag
    26401 atcaaagcgt taaacccggc aattccggtg ctgattatga ctgcgtactc cagcgtcgag
    26461 acggcggtag aggcactgaa aactggggcg ctggattatc tcatcaagcc gctggatttc
    26521 gataacctac aggcgacgct ggaaaaggcg ctcgcgcata cgcacagtat tgatgctgaa
    26581 acacctgcgg tgactgccag ccagttcggt atggtcggta aaagcccggc gatgcaacac
    26641 ctgctcagtg aaatcgccct cgtcgcgcca tcggaagcca cagtgctgat ccacggcgat
    26701 tccggcaccg gtaaagagct ggtcgccagg gcgattcacg ccagtagcgc acgtagcgaa
    26761 aaaccgctgg taacgctcaa ctgtgcggca ctcaacgaat ccttgctgga atctgaattg
    26821 ttcggtcacg aaaaaggggc gtttactgga gccgataaac gccgggaagg gcgctttgtt
    26881 gaggcggacg gcggcacgct gtttctcgat gaaattggcg atatctcgcc gatgatgcag
    26941 gtgcgtctgc tgcgtgcgat tcaggagcgc gaagttcagc gtgtcggtag caaccagact
    27001 atctcggttg atgtccggct gattgcggcg acccatcgcg atcttgccgc agaggtgaat
    27061 gccgggcgtt ttcgccagga tctctactat cgcctgaatg tggtggcgat tgaagtacca
    27121 tcgctgcggc aacggcggga agatatccct ctgctggctg gccattttct gcagcgcttt
    27181 gccgagcgta atcgcaaggc ggtaaaaggt tttacgcccc aggcgatgga tctgttgatt
    27241 cattacgact ggccgggaaa tattcgtgag ctggaaaacg cggtggaacg ggcagtggtg
    27301 ctgctgccgg ggaatatatt tccgaacgcg agctgccgct ggcgattgcc agtacgccga
    27361 tcccgctggg gcaaagtcag gatattcagc cgctggtgga agtggaaaaa gaggtgattc
    27421 tggcggcact ggagaaaacg ggcggcaaca aaactgaagc cgcccgtcag ttagggatca
    27481 cgcgtaaaac gttattggca aaactgtcgc gttagttctg ctcgcgttcg atagcacgcc
    27541 agccgatatc tttccggcag aagcagtcgt cccagtggat atcggtcatt aaggcatagg
    27601 cgcgtttctg cgcttctgcc acggtatgac ccagcgcggt gacgcacagt acgcgcccgc
    27661 cgttggtcac tacctgctcg tcatccgcca gttttgtgcc cgcgtggaac actttgccgc
    27721 ctgccacttc ttccagcagc aggccgtgga tcacgtcacc ggtgcggtaa tcacccggat
    27781 atccacccgc agccatcacc acgccgagag aagcgcgttc atcccactcg gacgttttct
    27841 cgtccagttt gctttcacag gccgccaggc agagctcaac cagatcggac ttcatgcgca
    27901 gcataatcgg ctgggtttcc ggatcgccaa agcggcagtt aaattcaata accttcggat
    27961 tgccctgttt gtcgatcatc aggcccgcgt agagaaaacc ggtgtaggtg ttgccttccg
    28021 ccgccatgcc tttcacggtt ggccagataa tacgttccat ggtgcgctga tgaacgtcat
    28081 cggttactac cggcgcgggg gagtaagcgc ccatcccgcc ggtgtttggt ccggtatctt
    28141 tatcgcctac gcgtttgtga tcctggctgg tagccatcgg cagcacatgc tcgccgtcca
    28201 ccatcacgat aaagctcgct tcttcgccat cgaggaactc ttcgataacg atgcgatgac
    28261 ccgcgtcgcc aaaagcgttg cccgccagca tatcgtgaac agccgcttcc gcttcttcca
    28321 gcgtcatcgc cacgataacg cctttcccgg cagccagacc gtccgcttta atgacgattg
    28381 gcgcgccttt ctcacgcaga tacgccagcg caggttctac ctcggtgaag ttctggtatt
    28441 ccgccgtagg gatcttatgg cgggccagga aatctttggt aaacgctttt gagccttcca
    28501 gttgggccgc acctgcggtt gggccgaaga ttttcagccc ggcggcgcgg aaggtatcga
    28561 ccacgccttt caccagcggc gcttccgggc cgacgatggt cagatcaatc ttttcgtttt
    28621 gtgcgaaatc caacagcgcc gggatatcgg tcacgccaat agcaacgttt tgcagcgcgg
    28681 gttccagcgc agtgcctgca ttacccggag caacaaaaac agtctcaacc agcggcgact
    28741 gggccgcttt ccaggccagc gcgtgctcgc gcccgccgtt accaatcact aatactttca
    28801 tctattgctc cattaatggc ggaagtggcg catgtcggtg aagagcatcg caataccgtg
    28861 ctcgtcggcg gcggcaatca cttcgtcatc acggatggaa ccgccaggct ggattacgca
    28921 ggtcacgccc gcagcggcgg cggcatcaat accgtcgcgg aacgggaaga acgcgtcaga
    28981 agccatcgag gaacctttca cttccaggcc ttcatcggca gctttaatac cggcgatttt
    29041 cgcggagtac acgcggctca tctggcccgc gccaataccg atggtcatat tgtttttggc
    29101 atagacgata gcattggatt tcacgaactt cgccactttc cagcagaaca gcgcatcgcg
    29161 cagttcctgt tcggtcggct gacgttgggt gacgacgcgc agttcttccg caccgaccat
    29221 ccccaggtca cgatcctgaa ccagcagacc gccgttcacg cgtttgaaat caagacccgg
    29281 aacacgctcg ccccactgac cgcaggtcag aacgcgtacg ttctgcttgg cggcggtgat
    29341 tttcagggct tcttcgctgg cggaaggcgc aataatcact tcgacaaact gacgagaaat
    29401 gatggcctgt gcggtttccg catctagctc gcggttaaag gcaatgatgc cgccgaatgc
    29461 ggaggttggg tcggttttgt acgcgcgatc gtaagcatca agaatggaat tgccgatagc
    29521 tacaccgcaa gggttggcgt gcttcacaat cacacatgcc ggctcggcga actctttcac
    29581 gcactccagc gccgcatcgg tatcggcgat gttgttataa gagagggctt taccctgaac
    29641 ctgggttgcg gtagcaacgg aggcttcttt cacattctct tctatataga aggcagcctg
    29701 ctggtggctg ttctcgccgt aacgcatatc ctgcttctta atgaagttca ggttcagcgt
    29761 gcgtgggaag cgaccggcgg cttctttgct ttcaccgtgg tacgccggaa ccatgctgcc
    29821 gaagtagttg gcaatcatgc tgtcgtaggc ggcagtgtgt tcgaaggctt tgatggcgag
    29881 gtcgaaacgg gttgcaagcg tcagcgatcc ttcgttgtca tccatctctt taataatggc
    29941 gtcgtagtcg ctgctcttca ccacgattgc gacatcttta tggttcttag cggcggagcg
    30001 caccatggtc gggccgccga tatcgatgtt ctcaaccgca tcttccagcg agcagccttc
    30061 acgagcaaca gtctgggcga acggatacag gttaacaacc accatatcga taggctggat
    30121 ctgatgttct tccataatgg catcgtcctg gccgcgacgg cccagaatgc caccatgtac
    30181 tttcggatgc agggtcttca cgcgtccatc catcatctcc gggaaaccgg tgtaatcgga
    30241 aacttcggtt accggcagac ctttttctgc taacagacgg gcagtgcccc ctgtagacag
    30301 cagctccaca ccgcgtgcgg aaagtgcctg ggcgaattcg acgataccgg ctttgtcaga
    30361 aacactgagc agagcgcggc ggactggacg acgttgttgc atggtaaatc ccctggattt
    30421 gactattaca gagagcgtta gctgaatttt tcgcgaaaaa ctcagctaac gcccctaacg
    30481 gggcatcctt atttttcgcc cgcattgtaa cgaaaacgtt tgcgcaacgc tcgcgaattt
    30541 ttctctttca atggtgatca caattttgac agtggctacc gtgggaaaaa tacagaaatt
    30601 acattgatga ttgtggataa ctctgtgcgt aaaaaggtat aaagcggggt tttgctgggg
    30661 aatgcagcag tcagtcattt ttctgcaatt tttctattgc ggcctgcgga gaactcccta
    30721 taatgcacct ccatcgacac ggcggatgtg aatcacttca cacaaacagc cggttcggtt
    30781 gaagagaaaa atcctgaaat tcagggttga ctctgaaaga ggaaagcgta atatacgcca
    30841 cactcacgac agtgcgctaa agctcgtcgc
//
LOCUS       DAZPEB010000027        28224 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZPEB010000027 DAZPEB010000000
VERSION     DAZPEB010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28224
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.027"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..856)
                     /locus_tag="ACHURJ_001097"
     CDS             complement(<1..856)
                     /locus_tag="ACHURJ_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HFR1280132.1"
                     /translation="MVKKPQQGTIFAAAQRSDSYFVKCINMLSLYEKIKIRLIILFLL
                     AALSFIGLFFIINYQLVSERAVKRADSRFELIQKNVGYFFKDIERSALTLKDSLYLLK
                     NTEEIQRAVILKMEMMPFLDSVGLVLDDNKYYLFSRRANDKIVVYHQEQVNGPLVDES
                     GRVIFADFNPSKRPWSVASDDSNNSWNPAYNCFDRPGKKCISFTLRINGKDHDLLAVD
                     KIHVDLNWRYLNEYLDQISANDEVLFLKQGHEIIAKNQLAREKLIIYNSEGNYNIIDS
                     VDTEYIAKT"
     gene            complement(956..1867)
                     /gene="gsiD"
                     /locus_tag="ACHURJ_001098"
     CDS             complement(956..1867)
                     /gene="gsiD"
                     /locus_tag="ACHURJ_001098"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFR1280133.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1870..2790)
                     /gene="gsiC"
                     /locus_tag="ACHURJ_001099"
     CDS             complement(1870..2790)
                     /gene="gsiC"
                     /locus_tag="ACHURJ_001099"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFR1280134.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILSSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2808..4346)
                     /gene="gsiB"
                     /locus_tag="ACHURJ_001100"
     CDS             complement(2808..4346)
                     /gene="gsiB"
                     /locus_tag="ACHURJ_001100"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFR1280135.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILVH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQAALLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(4366..6237)
                     /gene="gsiA"
                     /locus_tag="ACHURJ_001101"
     CDS             complement(4366..6237)
                     /gene="gsiA"
                     /locus_tag="ACHURJ_001101"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFR1280136.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLCVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(6224..7189)
                     /gene="iaaA"
                     /locus_tag="ACHURJ_001102"
     CDS             complement(6224..7189)
                     /gene="iaaA"
                     /locus_tag="ACHURJ_001102"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFR1280137.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPAIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIGHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            7393..8628
                     /gene="moeA"
                     /locus_tag="ACHURJ_001103"
     CDS             7393..8628
                     /gene="moeA"
                     /locus_tag="ACHURJ_001103"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFR1280138.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            8628..9377
                     /gene="moeB"
                     /locus_tag="ACHURJ_001104"
     CDS             8628..9377
                     /gene="moeB"
                     /locus_tag="ACHURJ_001104"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFR1280139.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLIVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHNDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEYDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(9453..10115)
                     /gene="fsa"
                     /locus_tag="ACHURJ_001105"
     CDS             complement(9453..10115)
                     /gene="fsa"
                     /locus_tag="ACHURJ_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFR1280140.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKNPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWLGAFGRT
                     SI"
     gene            10246..11145
                     /locus_tag="ACHURJ_001106"
     CDS             10246..11145
                     /locus_tag="ACHURJ_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFR1280141.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEIATALLQACHEAGIHTAVETC
                     LHVPWKYITPSLPYIDLFLADLKHIADAPFKQWTDGNAAKVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APDLLDFAQQYACQKGLTATLRG"
     gene            11151..13583
                     /locus_tag="ACHURJ_001107"
     CDS             11151..13583
                     /locus_tag="ACHURJ_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFR1280142.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLAIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQTLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGNVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13729..14544
                     /gene="ybiV"
                     /locus_tag="ACHURJ_001108"
     CDS             13729..14544
                     /gene="ybiV"
                     /locus_tag="ACHURJ_001108"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFR1280143.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIAFDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14696..15961
                     /locus_tag="ACHURJ_001109"
     CDS             14696..15961
                     /locus_tag="ACHURJ_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFR1280144.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSTSRPEIYPIYWSQTQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     HEDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(16202..17794)
                     /locus_tag="ACHURJ_001110"
     CDS             complement(16202..17794)
                     /locus_tag="ACHURJ_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFR1280145.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLMKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            18013..18933
                     /gene="ldtB"
                     /locus_tag="ACHURJ_001111"
     CDS             18013..18933
                     /gene="ldtB"
                     /locus_tag="ACHURJ_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR1280146.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(18992..20110)
                     /locus_tag="ACHURJ_001112"
     CDS             complement(18992..20110)
                     /locus_tag="ACHURJ_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFR1280147.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKVLQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLASLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(20107..20574)
                     /gene="mntR"
                     /locus_tag="ACHURJ_001113"
     CDS             complement(20107..20574)
                     /gene="mntR"
                     /locus_tag="ACHURJ_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFR1280148.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGTK"
     gene            20760..20888
                     /gene="mntS"
                     /locus_tag="ACHURJ_001114"
     CDS             20760..20888
                     /gene="mntS"
                     /locus_tag="ACHURJ_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFR1280149.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLILLSMLCDMVNNKPQQDKPSDK"
     gene            21160..22743
                     /gene="opgE"
                     /locus_tag="ACHURJ_001115"
     CDS             21160..22743
                     /gene="opgE"
                     /locus_tag="ACHURJ_001115"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFR1280150.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(22792..23307)
                     /gene="ompX"
                     /locus_tag="ACHURJ_001116"
     CDS             complement(22792..23307)
                     /gene="ompX"
                     /locus_tag="ACHURJ_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFR1280151.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(23360..23425)
                     /locus_tag="ACHURJ_001117"
     CDS             complement(23360..23425)
                     /locus_tag="ACHURJ_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1280152.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            23660..24547
                     /gene="rhtA"
                     /locus_tag="ACHURJ_001118"
     CDS             23660..24547
                     /gene="rhtA"
                     /locus_tag="ACHURJ_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFR1280153.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            24846..25349
                     /gene="dps"
                     /locus_tag="ACHURJ_001119"
                     /gene_synonym="pexB"
     CDS             24846..25349
                     /gene="dps"
                     /locus_tag="ACHURJ_001119"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFR1280154.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            25753..26499
                     /gene="glnH"
                     /locus_tag="ACHURJ_001120"
     CDS             25753..26499
                     /gene="glnH"
                     /locus_tag="ACHURJ_001120"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFR1280155.1"
                     /translation="MKSVLKVSLAALTLVFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            26638..27297
                     /gene="glnP"
                     /locus_tag="ACHURJ_001121"
     CDS             26638..27297
                     /gene="glnP"
                     /locus_tag="ACHURJ_001121"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFR1280156.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            27294..28016
                     /gene="glnQ"
                     /locus_tag="ACHURJ_001122"
     CDS             27294..28016
                     /gene="glnQ"
                     /locus_tag="ACHURJ_001122"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFR1280157.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
BASE COUNT         6978 a         7540 c         6973 g         6733 t
ORIGIN      
        1 atgtctttgc aatatactcc gtatcgacag aatcaataat attataatta ccttcgctat
       61 tataaataat cagtttttca cgagcgagtt gattcttggc aatgatctca tgaccttgtt
      121 tcaaaaatag aacttcatca ttagcgctga tttgatcaag atactcgttc aaataccgcc
      181 agtttaaatc gacatgaatt ttatctaccg ctaacaaatc gtgatctttg ccgttgatac
      241 gtagcgtaaa agagatacat tttttacccg gacgatcaaa gcaattgtat gccggattcc
      301 agctgttgtt agagtcatct gaagccaccg accacggtcg tttcgatggg ttaaaatcgg
      361 caaaaataac ccgccctgac tcgtcgacaa gcggtccatt tacttgttcc tgatgataaa
      421 caacgatttt atcattcgcc ctccgtgaaa agagataata tttattatca tcaagtacca
      481 gtcccaccga gtctaaaaat ggcatcattt ccattttcag aatcacggcg cgttgaatct
      541 cctctgtatt ttttaataaa tacagtgagt cctttaatgt cagggccgaa cgttcaatat
      601 ctttaaagaa atagccaacg tttttctgaa taagttcaaa gcggctatcg gcacgtttta
      661 ccgcgcgttc cgataccagt tgatagttaa tgatgaaaaa aagaccaata aatgacagtg
      721 ctgccaataa aaataaaatt atcagcctta tctttatctt ttcgtataaa ctcagcatgt
      781 taatacattt aacaaaataa ctatctgaac gctgtgccgc tgcaaaaata gtcccttgct
      841 gcggcttttt aaccaaatta cccgattcta caaaatcagc agaaagttac aacacttgct
      901 tagggcaata gtttcctgtg aggtgattac cctttcaagc aatattcaaa cgtaactatc
      961 ctttaatttt cggatccagc gcatcgcgta aaccatcgcc caacaaattg aacgccagta
     1021 cggtcagaaa aatagccagg gccggaaaaa cagcgacatg cggcgcgata accatatccg
     1081 ctcgtgcctc attgagcatt gctccccact ctggtgtcgg cggctgcgca ccgaggccga
     1141 gaaatgacag gctggcggca gagataatcg aggtgccaat gcgcatggtg aaaaacacca
     1201 cgatagacga gacggtccca ggcaagatat gacgcaataa aatggtcata tcgctggcac
     1261 caatactgcg tgctgactca ataaaggttt gctgtttcaa caccagcgtg ttgccgcgca
     1321 ccagtcgggc aaacgcgggg atggaaaaaa tggcgactgc gataatcacg ttagcaatgc
     1381 cgcttcccaa caccgcaaca acagcgatcg ccagtaaaat acccgggaag gcaaacagca
     1441 catcgcaaat gcgcatgatc agccgatccc accagccttc ataatatcca gccaacaagc
     1501 ccagcaacgt cccgatcgcc gcaccgataa acacggcaaa cacgcccgcc gccagcgaga
     1561 tttgcgcacc aaccaggaca cggctgaaaa tgtcgcgccc cagtgaatcg acgccaaacc
     1621 agtgctgcaa agaaggtccg ttattcagat tgtcataatc aaaataattt tcggcgtcat
     1681 agggagcgat ccagcgtgca aaaatggcca ccacaatcaa taaaataacg aataatgcgg
     1741 cggtcatcgc catatgctga cggcgaaatc gtcgccagaa ttcatgccac ggtgtacgca
     1801 cctggtcagg tttgaccagt ggcatggcgt ttaacaccgc ctgacgtcgc cagttaaata
     1861 gtcgcatcct tacttgtacc tgatagccgg gttaatggcg gcgtaaagca catccaccac
     1921 taagttgata agaataaatt ccagcgagaa aagcagaatt tccgcctgaa tcaccgggta
     1981 atcgcgcatt tctacggagt caacgagtaa gcgtccaagt ccgggccagt tgaaaacttt
     2041 ctccaccacg atagaaccac cgagcaaaaa gccaaactgt aagcccatca tggtcactac
     2101 cgggatcatc gcgttacgta gcccgtgttt gaggacaacc caggtttcgc tcaccccttt
     2161 cgccctcgcg gtacgcatat aatcttcgct taaaacatcg acaaacgacg cgcgggtaaa
     2221 gcgcgccatc acggctgcca ctgccgcgcc gagcgtcagg gaggataaaa tgtagtgctg
     2281 ccaactgtct gctcccacgg taggcagcca gcccagttca acggagaata cctgaattaa
     2341 aagcatcccc agcgcaaatg ccggaaacga gatccccgac accgcaatgg tcatactcaa
     2401 tcgatccggc caacgattac gccagacggc ggcgataatt cccgccgcca taccaaatat
     2461 caccgcccag accatactgg ttatggtcag ccacagcgtt ggcataaagc ggctggcaat
     2521 ctcatcggca accggacgac gcgacaccat cgacaggcca aaatccccct gcacagcatt
     2581 gctgatatag tgccagaact ggtgatacag cggctgatcc aaccccagct gctgacgcac
     2641 cagttctata acctgcgcat cagcttcggg cccggcaatc aatcgcgccg gatcgccggg
     2701 cagcatatgc acaaataaaa acaccagcac cgagacgata aacagcgtcg gaatcaaccc
     2761 cagtaagcgt ttgataacgt aattaagcat tccactccct gcgttgctta ttgcaaatcc
     2821 gcgtcttcaa agctgaagcc ggtgtctggc atgatccaaa aaccggtcag gtttttactg
     2881 tgtgccgaca ccagtttttc taccaccagc gggatccacg gcgactcttg ccagatgata
     2941 tcctgcgccg ccttatacaa gcgggtcttt tccgccggat cattagtttt cagtgcctga
     3001 gccaggaagt cgtccacctg tttattgctg taaaatgcgg tattaaacag cgtcggtggc
     3061 cagttttgcg aggcgaacag tggcgatagc gcccagtccg cttcgccggt tgaagccgac
     3121 cagccagtgt agaacatccg cacgccgctc tctttttgcc ctttaccttc aacttctgcc
     3181 gcccgctgtc cggcatccat cgcggtcacc tgggctttaa tcccgacctg cgctaactgc
     3241 tgctgggtaa attgcagcac tttctgcgcg gtgctgtggt tatgtgacga ccacagcgtg
     3301 gtactgaaac cgttgggata tcccgcctct ttcagtaatt cgcgcgcttt cactggatcg
     3361 taaggccacg gtttataact ttgcgcgtag gcgatacttg gcggtaccac accagtagct
     3421 ggcgttgcat agcccgcaaa ggcaactttc accagcgccg gacggttaat ggcgtaattc
     3481 agcgcctcac ggaccttcgg gttatcgaac ggcttttgcg tcacgttcat actgatataa
     3541 cgctgcataa ttgacggact ggccatcaac tcgatatttt tgtttttctc cagcagtgcg
     3601 gcctgctcgt agggaatggg gaaagcaaac tgcgcttcac cggtttgcag cattgccgcg
     3661 cgggtgttgt tatccgccac cggacgccag gttatgctgt ccagtttggg caatcctggc
     3721 tgccagtaac ccgcgaattt tttcaccttc acaaaatcgg tctgattcca ggtatccagt
     3781 tcatacggtc cggttcccac cggatgaaaa ccaatctcct tgccatattt ttccagcgct
     3841 gccggtgaaa tcatcgcggt cgccggatgg acaagaatat taataaacgc tgagaacggc
     3901 tgtttgaggg taatctttac cgttgtcgga tcgatcgcct ccgttttggc aatattctta
     3961 tacaggttat agcgtttaag atgattcgcc ggatcgctgg cccggtccag attcgctttc
     4021 accgccgcgg cgttgaaatc ggtgccatcc tggaatttaa ttccttcccg caatttcacg
     4081 gtgtaagtaa tgccgtcatc ggaaacggta taactctccg ccagcacgtt tttcagtttc
     4141 atctctttat ccagaccgaa cagcccctgg taaaacgatt tcgctacggc ctgagataac
     4201 gtgtcattcg cgtcatacgg atcgagcgtg gtgaaattcg atcctaccgc caccaccaca
     4261 tctttggcag cgaatgcaca agatgccatc aacgctgtcg caatgcccag cgccactaac
     4321 ccactacggt gtacagctct tgccatttta ttctccgcct gaatgttatc tacgcatgaa
     4381 tgcgtattct gattgtggtt gtgcgacgta atgccccggc ccgacgcatt gcaacgagac
     4441 ggctgccacc tcttcgccac gcagatgaat attgctggga agatcgtccg acagcagtac
     4501 acgctgcggt cgttgtcggg acggttcagc gaccggaact gccgccagta atttacgcgt
     4561 ataaggatgc tgcgggtttt cgaagaccgc gcgccgtgga ccaatttcaa caatttgccc
     4621 gagatacatc accgccacac gatgactaat ccgctctacc acggccatat cgtgggagat
     4681 aaacagatac gcaatgccga aatcacgctg gagatcgagc aacaagttga taatctgccc
     4741 gcgaatagaa acatccagcg ccgaaacggc ttcgtcggca atgatcactt ttggattcaa
     4801 tgccaacgcg cgagcaatgc agatgcgctg gcgctgaccg ccggaaaact catgcgggta
     4861 acgccaggca tgttcaggta acaggcccac gcgctccagc aaccacgcaa cgcgtgcagc
     4921 cgcgtcttta cctggcaata aaccgtgtac acacagcggt tcgataatcg aatcaccgat
     4981 ggtctgacgt gggtccagcg aagcgtaagg gtcctgaaaa ataaactgaa tatcccggcg
     5041 caatgcctga agtttgccag gtgacaaggt atcgattcgc tgaccgttaa agataatttc
     5101 gccgccctgc gattcgacca ggcgcagcaa cgcccgcccg gtagtggatt taccgctgcc
     5161 agactcgccc accagcgata gcgtttcgcc aggccagaga tcaaaactga ctttctcaac
     5221 ggcatgcact tcccgcgtta cgcgattcaa caaaccgctg cgcaaaggga aacgggtgac
     5281 aagattacgc actcgtaaaa caggttcgcc atccaccacc gttttctgct cgatgggggg
     5341 ttcctgtttc gctggatgtt caagcgatat caatgggaaa cgtcggggat aatctaaccc
     5401 tttcatcgca ccaagttgcg gaacagcagc taacagcgca cgggtgtaag gatgttgcgg
     5461 tgcatgaaaa atctgttcga cggtacccgt ttccaccgcc tcgccctgat acatcaccag
     5521 tacccgatcg gctatctctg ccaccacgcc catatcgtgc gtgataaaga taacacccat
     5581 cgacatctct ttttgcaata ctttgattaa ttgcaggatc tgcgcctgaa tagtgacatc
     5641 cagcgcggtg gttggctcat cggcaatcag caccgccggg cggcatgaca gcgccatcgc
     5701 aatcatcact cgctggcgca tcccgccaga gagttgatgc ggataacgtg aaagaatggt
     5761 ttgtgcctca ggaatgcgta cctgatccag catccgcttc gcctcgacca tcgcttcttc
     5821 acgactggcg ttctgatgca gacgaattga ttcggcaatc tgttcaccca cagtaaatac
     5881 cgggttcagc gatgtcatcg gctcctgaaa tatcatcgcc atatccgcac cgcgaacatg
     5941 gcgcatttgt gcagcgctct gctcgctaag ttcaatcact tcgcgactgc gccgctgcaa
     6001 cagcatttta tcgcactgta ctaaaccgcc cgcctgttcc aacaggcgca tcaatgccaa
     6061 cgcagtcact gacttaccgg agccggattc gccaacaatt gccagcgtct caccgcgttg
     6121 cagactaaaa gagagattgc ggaccgcagc tattttctgc tggtcctgca taaaggcaat
     6181 attcagattt tcaaccgcca gcacattacc ggcatcaagt tcatcactgt gtggcaacgg
     6241 tgtccccttt ttcacggtag ataccggtgg ttggcgtatc gcctgcgtag ccccaggcgc
     6301 gatacattcc ttcggtgtta aacggtagcg cgacattccc ttcatggccg atagcgatta
     6361 agccaccgct accgccaagc gcagggagtt tttccattac tacccgctcg caggcttccg
     6421 cgagacttaa tccgccgtaa tccattaacg cggcgatgtc atatgccgcc agcgcgcgga
     6481 tgaagacttc gcccgtgccg gtacaagaaa ccgccacact ggcgttattg gcgtagcatc
     6541 cggcacccac taagggacta tcgccaactc gtccgggtaa tttattggtc attccgcctg
     6601 tggacgtggc tgccgccaaa ttgccgtcta aatccaacgc cacggccccc acggtgccca
     6661 ttttctgttt ttcatccagt ggcgcaccgc tatggtcgag gacggttgcc ccttccttgc
     6721 gcgctgccag tagttgttca taacgcaaag gcgtggagaa aatcgccggc gagacgcgct
     6781 ccatgccacg agcaaacgca aaattttctg ccccttcgcc aatcatcatc acatgcgggc
     6841 tttgctccat caccagccgg gcggcaagaa ccggattacg cagatgacta acgcccgcca
     6901 ccgcaccggc tttcagggtg ttaccatcca tcacacaggc gtccagttca tgggtttcat
     6961 cacgcgtaaa gacagcgcca attccggcgt taaacagtgg acactcttcc agcagacgca
     7021 ccgcttccgt caccacatcc agcgcacttt cgcccgcttc cagcattttc tgcccggttt
     7081 caacaatggc agacaacgcc tcaatgtagc gtaattcctg ttgcagactc atctgcgcgc
     7141 ggctaattgc acctgcgcca ccatgaattg caatgactgc tttgcccata gtattcgtcc
     7201 attaagggtg gctcgccact tttgctataa atatcagaat ttttatgaat tactcattca
     7261 atttttgaat atagaaagat ggatatggca tgtaaaggca ggagccaacg ctacgcccga
     7321 ggtggcatac agtctgtggc gtttttctga cataataggc aaattcgatt ttgcctccgc
     7381 aggagtgttt tcatggaatt taccaccgga ttgatgtcgc tcgacaccgc gcttaatgag
     7441 atgctttctc gcgtcacccc actgaccgcc caggaaacgc tgccactggt acagtgtttt
     7501 ggtcgtattc tggcgagcga tgtcgtttcg ccgcttgatg ttccggggtt tgataactcc
     7561 gcaatggacg gctacgcggt gcgtttagcc gatattgcct ccgggcaacc gctgcccgtt
     7621 gccggtaaat cctttgccgg tcagccgtat catggtgaat ggcctgcggg cacctgcatt
     7681 cgtattatga ccggtgcgcc ggtgccggaa ggctgcgaag cggtggtgat gcaggagcag
     7741 actgaacaaa cggacaatgg cgtgcgtttt actgctgaag tgcgtagcgg gcaaaatatt
     7801 cgccgtcgcg gtgaagatat ctctgcaggt gcggttgttt tcccggcggg aactcgcctg
     7861 actaccgcag agctgccagt aattgcttca ctggggattg ccgaagttcc ggtgattcgt
     7921 aaagtgcgtg tagcgctttt ttctaccggt gatgaactcc agttgcccgg tcagccgctg
     7981 ggtgacggcc aaatctacga caccaaccgt ctcgccgtac acctgatgtt agaacagctg
     8041 ggttgcgagg taatcaactt agggattatc cgcgacgatc cccatgccct gcgcgccgca
     8101 tttattgaag ccgacagcca ggcggatgta gtgatcagtt ccggcggtgt ttcagtgggt
     8161 gaagcggatt acaccaaaac gattcttgaa gagctggggg agatcgcctt ctggaagctg
     8221 gcgattaaac caggtaaacc gttcgcgttc ggtaaactca gcaatagctg gttctgcggc
     8281 ctgccgggca acccggtttc agcgacgctg accttctatc aactggtaca gcctttgctg
     8341 gcaaaactaa gcggcaacac cgccagcggc ctgcccgcgc gccagcgcgt acgcacagcg
     8401 tcccgcctga aaaaaacgcc aggacgtctt gatttccagc gcggcgtgct gcaacgcaac
     8461 gccgacggtg aacttgaagt gacgaccacc ggacatcagg gttcacatat atttagctcc
     8521 tttagtctcg gcaactgctt tatcgtgctg gaacgcgatc gcggcaatgt ggacgtgggc
     8581 gaatgggtgg aagtagaacc gtttaacgcg ttgttcggag gcctgtaatg gcggaactca
     8641 gcgatcagga gatgctgcgc tacaaccggc aaatcattct gcgcggtttt gattttgacg
     8701 gtcaggaggc gctaaaagat tctcgtgtgc tgatcgtcgg cctcggtgga ctcggctgcg
     8761 cagcttcgca gtatctggca agcgccggtg tcggcaacct gacgctgctc gacttcgaca
     8821 cggtttcgct ctcgaatctg caacgccaga cactgcacaa tgatgccacg gtcgggcaac
     8881 cgaaggtgga atccgcccgt gacgccctga cgcggatcaa cccacatatc gcgattacgc
     8941 cagtcaatgc actgctagat gacgcagaac ttgcagcatt gattgctgaa tacgatctgg
     9001 tgctcgactg tacggataac gttgcggtac gtaatcaact gaacgcaggc tgttttgccg
     9061 cgaaggtgcc gctggtttcc ggcgcggcaa ttcgtatgga agggcaaatc acggtcttca
     9121 cttatcagga cggcgaaccg tgctatcgct gccttagccg tttgtttggc gagaacgcgt
     9181 taacctgcgt agaagcaggc gtgatggcac cgttgatcgg cgtaattggt tcgttgcagg
     9241 caatggaagc gatcaaactg ctggcaggtt atggcaaacc ggccagcggg aaaatcgtca
     9301 tgtacgatgc gatgacctgt cagtttcgcg aaatgaaact gatgcgtaat ccggggtgtg
     9361 aggtgtgcgg gcagtaattg ccacctccag gccagataag acgcggcact ccgccacgtc
     9421 atcagcaatt tatgaggatg cagaacgtcc ggttaaatcg acgttctgcc aaacgctccc
     9481 agccagtcct gttcaaactt cgccacagcg gcatcaaccg ccggatagct aatcatctgt
     9541 tgtgccacat ccagtggcag cgtaattgat tcacatcctg ccagtaagca gtccagcgcc
     9601 tgacgcgggt ttttgaaact cgctgccagc actttcgcct gcggcgcatg cattttcaat
     9661 aactggtgta agtcggtcac agtctgaatg ccgctaccgc cctgagcatc aatacgatta
     9721 acgtaaggcg caacatattc cgcacctgcc agcgccgaca gcagcccttg tgctgcgcca
     9781 tataccgcgg ttcccagcgt tggaatccct tccgctttta acatcttaat agctgccagc
     9841 ccctcggcgg tcaccggaac tttcaccacg atatccgcaa taatagaacg caatttacgt
     9901 gcgtcattca ccatcccttc agcagtggta gccattacct gggcaaacag acgcccctga
     9961 ccgcccatcg cttcatgaag ttgcggaagc acaacatcca gcggtttttt acccgcggcg
    10021 ataatgcttg ggttagtggt cacacccgcc agcggaaaaa tacgtgacag cgccttcact
    10081 gcaacaacgt ctgaagtatc cagatacagt tccataacca tcctcaaaat gtactttttg
    10141 tctgaatgta gactatcacg ggaaaactct ctgaacagtt gacgcacatc aatccaactt
    10201 tcattcgaaa gtattttaat gtttgaacga aacagagagg caactatgat tttcaatatt
    10261 cagcgctact cgacccatga tggccccggt atccgcacgg tcgtatttct taaaggctgt
    10321 tcgctgggct gccgctggtg tcagaacccg gaaagccgcg cccgcacgca ggatctgctg
    10381 tatgacgcac gactgtgtct ggaaggctgc gagctgtgcg ctaaggcagc gccggaagtg
    10441 attgagcgcg cattgaatgg tttgcttatt catcgggaaa agttaacccc ggagcatctg
    10501 acggcgctaa ccgactgctg cccgacacag gcgttaaccg tgtgtgggga agtgaaaagc
    10561 gttgaggaga tcatggcgac cgttctgcgc gataaaccgt tttacgatcg cagcggcggc
    10621 ggcttaacgc tttcgggtgg tgaacccttt atgcagccgg aaatagcgac ggcgctactg
    10681 caagcctgcc acgaggcagg cattcatact gcggtagaaa cctgtctgca tgtgccgtgg
    10741 aaatatatca ccccttctct gccctatatc gatctgtttc ttgccgattt aaaacacatt
    10801 gccgacgcgc cgtttaaaca gtggaccgac ggtaacgccg ccaaggtgct ggataacctg
    10861 aaaaaactcg ccgcagcggg caaaaaaatc attatccgcg tgccgctgat tcagggcttt
    10921 aatgccgacg aaacctctgt aaaagccatt accgattttg ccgccgacga gctgcacgtt
    10981 ggcgaaattc attttctgcc ctaccacacg ctgggcatca acaaatatca cttacttaat
    11041 ctgccctatg acgccccgga aaaaccgctt gatgcgccag acctgctcga ctttgcccag
    11101 cagtatgcct gccagaaagg gttaaccgcg accttacgag gataacaacc atgaccacac
    11161 tgaaactgga cacgctcagc gaccgcatta aagcgcacaa aaatgcgctg gtgcatattg
    11221 tgaaaccgcc ggtctgtacc gagcgcgcgc agcactatac cgagatgtat caacaacatc
    11281 tcgataagcc gatcccggta cgtcgcgcgc tggctctggc gcatcacctg gcgaatcgca
    11341 ccatctggat caagcacgat gagttgatca tcggcaacca ggcaagcgag gttcgcgctg
    11401 cgccgatctt cccggaatat accgtctcgt ggatcgaaaa agagattgat gatctggcag
    11461 atcgtcccgg tgctggtttt gcggtgagcg aagagaacaa acgcgttctg catgaagtgt
    11521 gcccgtggtg gcgcggtcag acggtacagg atcgctgcta cggcatgttt accgatgagc
    11581 aaaaaggtct gctggcgacc ggaatcatta aagcggaagg caatatgacc tccggcgatg
    11641 cgcacctggc ggtgaatttc ccgctgctgc tggaaaaagg tcttgatggt ctgcgcgaga
    11701 aagtagcgga acgtcgctcg cgcatcaatc tgacggtact ggaagatttg cacggcgaac
    11761 agttcctgaa agcgattgat atcgtgctgg tggcagtcag cgaacacatt gaacgttttg
    11821 cagccctggc gcgtgaaatg gccgcgaccg aaacccgcga aagccgtcgc gatgaactgc
    11881 tggcgatagc agaaaactgc gatcttatcg cccaccagcc gccgcagact ttctggcagg
    11941 cgctgcaact gtgttacttc atccagttga tattgcagat cgaatctaac ggtcactcgg
    12001 tatcgtttgg tcgtatggac cagtatctct acccgtacta tcgccgagac gttgaactca
    12061 accagacact ggatcgcgaa cacgccatcg aattgctgca tagctgctgg ctgaaactgc
    12121 tggaagtgaa caagatccgc tccggctcac actcaaaagc ctctgcggga agtcctctgt
    12181 atcagaacgt caccattggc ggacaaactc tggttgatgg tcaaccaatg gacgcggtga
    12241 atccactctc ttacgcgatc ctcgaatcct gcggtcgcct gcgttccact cagcctaacc
    12301 tcagcgtgcg ttaccatgca ggaatgagta atgatttcct cgacgcctgc gtacaggtga
    12361 tccgttgcgg cttcgggatg ccggcgttca acaacgacga aatcgtgatc ccggaattta
    12421 ttaaactcgg tattgaaccg caggacgctt acgactacgc agcgattggt tgtatcgaaa
    12481 ccgccgtcgg tggcaaatgg ggctatcgct gtaccggcat gagctttatc aacttcgccc
    12541 gcgtgatgct ggcggcgctg gaaggcgggc gtgatgccac aagcggcaac gtgttcctgc
    12601 cacaagaaaa agcgttgtcg gcaggcaact ttaacaattt cgatgaagta atggacgcgt
    12661 gggatacgca aatccgttat tacacgcgta aatcaatcga aatcgaatat gtcgtcgaca
    12721 ccatgctgga agagaacgtg cacgatattc tctgctcggc gctggtggat gactgtattg
    12781 agcgagcgaa aagtatcaag caaggcggcg cgaaatatga ctgggtttct ggcctgcagg
    12841 ttggcattgc caacctcggc aacagcctgg cggcagtgaa gaaactggta tttgaacagg
    12901 gcgcgattgg tcagcaacag ctggctgccg cactggcgga tgacttcgat ggcctgactc
    12961 atgagcaact gcgtcagcgg ctgattaacg gtgcgccgaa gtacggcaac gacgatgata
    13021 ctgtcgatac gctgctggct cgcgcttatc agacctatat cgacgaactg aaacagtacc
    13081 ataatccgcg ctacggtcgt ggtccggttg gcggcaacta ttacgcgggt acgtcatcta
    13141 tctccgctaa cgtaccgttt ggtgcgcaaa ctatggcaac gccggacggg cgtaaagccc
    13201 acaccccgct ggcagaaggc gctagtccgg cctctggtac tgaccatctc ggccctactg
    13261 cggtcattgg ctcggtgggt aaactgccta cggcagcgat tctcggcggc gtgttgctca
    13321 accagaaact gaatccggca acgctggaga acgaatctga caagcagaag ctgatgatcc
    13381 tgctgcgcac cttcttcgag gtgcataaag gctggcatat tcagtacaac atcgtttccc
    13441 gcgaaacgct gctggaggcg aaaaaacatc cggatcagta ccgcgatctg gtagtgcgtg
    13501 tcgcgggtta ttccgccttc ttcaccgcgc tctctccaga cgctcaggac gatatcatcg
    13561 ctcgtactga acatatgctg taatctcttc ggcccggctg cggcgtcgcc gggctaaatc
    13621 attcacatca tcaatttcat ccttactttc attcgaaata taatttgtgc tctgcgtcac
    13681 attgttatta cactgttacg gtttcatttt cgagccagga gcacacctat gagcgtaaaa
    13741 gttatcgtca cagacatgga cggtactttt cttaacgacg ccaaaacgta caaccaacca
    13801 cgttttatgg cgcaatatca ggaactgaaa aagcgcggca ttgagttcgt tgtcgccagc
    13861 ggtaatcagt attaccagct tatttcgttc tttcctgagc taaaggatga gatctctttt
    13921 gtcgcggaaa acggcgcact ggtttacgaa catggcaagc aactgttcca cggcgaactg
    13981 acccgacatg aatcgcggat tgttattggc gagttgctaa aagataagca actcaatttt
    14041 gtcgcctgcg gtctgcaaag tgcatatgtc agcgaaaatg cccccgaagc atttgtcgca
    14101 ctgatggcaa aacactacca tcgcctgaaa gctgtaaaag attatcagga gattgacgac
    14161 gtactgttca agttttcgct caacctgcca gatgaacaaa tcccgttagt gatcgacaaa
    14221 ctgcacatag cgttcgatgg cattatgaaa cccgtcacca gtggttttgg ctttatcgac
    14281 ctgattattc ccggtctaca taaagcaaac ggtatttcgc ggttactgaa acgctgggat
    14341 ctgtcaccgc aaaatgtggt agcgattggc gacagcggta acgatgcgga gatgctgaaa
    14401 atggcgcgtt attcctttgc gatgggcaat gctgcggaaa acattaaaca aatcgcccgt
    14461 tacgctaccg atgataataa tcatgaaggc gcgctgaatg tgattcaggc ggtgctggat
    14521 aacacatccc cttttaacag ctgacctctc taccggagcc gtgcgctccg gttttccctt
    14581 ctttttacat cctgtacatc acatcaaatc attaaacttg cattaactta tttataactt
    14641 atattgtcac atgcaaatca taatggtttc gaatgaaagt taatgagagg tggttatggc
    14701 ttctactttt accagcgaca cattgccagc cgatcacaaa gcagctatcc gtcagatgaa
    14761 gcacgcgctg cgggcgcagc ttggcgacgt ccagcagatc tttaatcagc taagcgatga
    14821 cattgccacg cgagtggctg aaatcaacgc actcaaagca cagggcgatg ccgtctggcc
    14881 ggtgctgtct tatgccgata tcaaagcagg tcatgttact gcagagcagc gcgaacagat
    14941 taaacgtcgc ggttgtgcgg tgataaaagg ccatttcccc cgcgaacaag cgctaggctg
    15001 ggatcagtcg atgctggact atctggaccg caaccgcttt gacgaggtct acaaaggccc
    15061 cggcgataat ttcttcggga cgctcagcac ttcacgtccc gagatttacc ccatctactg
    15121 gtcgcagacg caaatgcagg cccgccagag tgaagaaatg gcgaatgcgc agtcgtttct
    15181 caatcgtctg tggacatttg aaagtgatgg aaagcaatgg tttaacccgg atgtgagcgt
    15241 catctaccct gaccgtatcc gccgccgtcc gcccggaacg acctccaaag gtcttggagc
    15301 gcataccgac tccggggcgc tggaacgctg gctgcttcca gcgtatcagc gcgttttcgc
    15361 caacgtcttt aatggcaatc tggcgcaata tgatccctgg catgcggcac atcgtacgga
    15421 agttgaagag tacacggtgg acaacaccac caaatgttcc gtgtttcgga cattccaggg
    15481 ctggacagcg ctctctgata tgctgcctgg tcaggggttg ctgcacgttg tgcccattcc
    15541 tgaagccatg gcgtacgtac tgttacgtcc gctgcttgat gatgtgccgg aggatgaact
    15601 gtgcggcgta gcgcccggaa gagtgttgcc ggtatcagag caatggcatc cactgttaat
    15661 tgaggcgtta accagcattc caaaactcga ggccggagac tccgtctggt ggcactgcga
    15721 cgtcatccat tccgttgccc ccgttgaaaa tcaacagggt tggggcaacg tgatgtacat
    15781 tcctgcggca ccgatgtgcg agaaaaatct tgcctacgcg cacaaggtga aggccgcact
    15841 ggaaaaaggc gcatcgccgg gcgacttccc gcacgaggac tatgaaacaa actgggaagg
    15901 acgctttacg cttgccgacc tcaacattca cggtaagcga gcgttgggca tggatgtttg
    15961 agagttaatg gaagtgaatt gatgtgggat taagaaaatc gattcgacgt tactcagatt
    16021 gctgacaagt gcgctttctt aatgcctgat gcggcgttaa cgccttatct ggcctacaaa
    16081 gtcctgcaga ttcaatatat tacaggtact gcgtaggcct gataagcgta gcgcatcagg
    16141 ctattttgca tttgtcatca gccgccgtca ccccgacagt atcggggtga cggataatca
    16201 cttactcgat ccctttacta cgcaggtaat cttcgtaatt accgctaaag tcgatcacgc
    16261 gttccggggt gatttccaga atgcgggtcg ccagggagct tacgaactca cggtcgtgtg
    16321 aaacaaagat cagcgtgccc tgatacagtt ccagcgccat gttcagtgac tcgatggatt
    16381 ccatatccag gtggttggtc ggttcgtcca tgatcagaat gttcggcttc tgcatcatta
    16441 acttaccaaa cagcatccgc cctttttccc caccggaaag cactttagct ggctttttga
    16501 tgtcgtcctg gctgaacagc aaacgaccga gaatgctgcg taccgcctgc tcgtcatcgc
    16561 cttcctgctt ccactgactc atccattcga acacggtcag atcattttca aactcatatt
    16621 cgtggtcctg ggcgtagtaa ccaatgcgcg cgttttcaga ccattttacg gtgccgctgt
    16681 ccggttgcag atcgcccacc agcgttttca tcagcgttga tttaccgacg ccgttggtac
    16741 ccagtaccgc aagtttttca ccgacctcca gcagcaggtt gagattttta aacagtggac
    16801 cgttatcaaa ccctttggtc agaccttcca cttccagcgc gttacggaac agtttcttat
    16861 cctgttcaaa acggatgaac gggttctgac ggctggaggc tttcacctct tccagtttga
    16921 ttttatcaat ctggcgcgcg cgcgaagttg cctggcgaga tttcgaggcg ttggcgctaa
    16981 agcggctaac gaaagattgc aactcagcaa tctgcgcttt cttcttggcg ttatcggcca
    17041 gcagacgttc acgcgcctgg gtcgccgccg tcatgtactc atcgtagtta cccggataaa
    17101 cgcgcagctc gccgtaatcc agatccgcca tgtgggtaca gaccatgtta aggaagtgac
    17161 ggtcgtgcga gatgatgatc atggtgctgt cacgctcgtt cagcacctgt tccagccagc
    17221 gaatggtgtc gatgtcgagg ttgttggtcg gttcgtcgag caggagaata tccggatcag
    17281 caaacagcgc ctgcgccaga agcacacgca gcttccagcc aggagcaact tcactcatcg
    17341 ggccgtagtg ctgttccact ggaattccca cgccaagcaa caattcaccg gcgcgagctt
    17401 ccgcagagta accgtccatt tcgccgtatt taacttccag atcggccact ttatagccgt
    17461 cttcctcact catttccggc aaagcataga tgcggtcacg ctcctgcttc acttcccaca
    17521 actctttatg ccccatgatc accgtatcca gcacagtgaa ctcttcaaag gcaaactgat
    17581 cctgacgcag cttaccaatg cgctcgttgg gatcgaggga aacgttaccc aaagtcggct
    17641 ctaagtcgcc gccgaggatc ttcataaagg tggatttacc actaccgttc gcgccaatca
    17701 ggccgtaacg gttgccgccg ccaaatttga cggaaatgtt ttcaaacaac ggcttactgc
    17761 cgaactgcat ggtgacgtta ctggaaacta acacaggcgt atcctgaaaa gagatatgac
    17821 aaaccgcgca ttatgccata agtctgaaag aagatcctgc tcctcgctta aggtctacac
    17881 tcaagccaaa ggcaaaaaat gtgatttcgt acacatctga tttcactgtg agctggaatg
    17941 aacttataat gcgcttccaa tactctaata ttctcaaccc aatggcctgc caggcacaaa
    18001 atctcgctta acatgaatat gaaattgaaa acattattcg cagcggcctt cgctgttgtc
    18061 ggcttttgca gtaccgcctc tgcggtaact tatcctctgc caaccgacgg gagtcgcctg
    18121 gttggtcaga atcaggtgat caccattcct gaaggtaaca ctcagccgct ggagtatttt
    18181 gccgcggagt accagatggg gctttccaat atgatggaag cgaacccggg tgtggatacc
    18241 ttcctgccga aaggcggtac tgtactgaac attccgcagc agctgatcct gccggatacc
    18301 gttcatgaag gcatcgtcat taacagtgca gagatgcgtc tgtattacta tccgaaaggg
    18361 accaacaccg ttatcgtgct gccgatcggc attggtcagt taggcaaaga tacgcctatc
    18421 aactggacca ccaaagttga gcgtaagaaa gcaggcccga cctggacgcc gaccgcaaaa
    18481 atgcacgcag agtaccgcgc tgcgggcgaa ccgcttccgg ctgtcgttcc ggcaggtccg
    18541 gataacccga tgggtctgta tgcactctac atcggtcgcc tgtatgctat ccatggcacc
    18601 aacgccaact tcggtatcgg cctgcgtgta agtcatggtt gtgtgcgtct gcgtaacgaa
    18661 gacatcaaat tcctgttcga gaaagtaccg gtcggtaccc gcgtacagtt tattgatgag
    18721 ccggtaaaag cgaccaccga gccagacggc agccgttata ttgaagtcca taacccgctg
    18781 tctaccaccg aagcccagtt tgaaggtcag gaaattgtgc caattaccct gacgaagagc
    18841 gtgcagacag tgaccggtca gccagatgtt gaccaggttg ttcttgatga agcgattaaa
    18901 aaccgctccg ggatgccggt tcgtctgaat taatcttcaa aacttaaagc aaaaggcgga
    18961 ctcataatcc gcctttttta tttgccagac cctagttggc cgggagtata actaacaaaa
    19021 catatcccac caacgccgcc cacaacagca tcggtattga atagagatgg aaacgccacc
    19081 agatgcggcg atcgttcgcc atacgtagcg caatcaaact tgccagcgat ccgggtaata
    19141 acccaaagcc acctacgttt accgcccatg ccagtaataa agacggcggc acatagttca
    19201 gcaacaatat ggtactcggc acgttactga tcacctgcga taaaccgatt gccgttaacc
    19261 ataacccggg ttcagataga tgactcacgt tacccaacac gccttgcaac gctggaagct
    19321 gggtcagtaa atggacgtcg ataaacatcg ccataaacac cagcagcagc gtccagtcca
    19381 cactgagcac cacgcgacgt gccagcagcg caaagcctgc cgccacaatc accagtcccc
    19441 acagctcttg tttgaactcc agcgccgtta gaaagacgat atacagcccc agacaactcc
    19501 acaccagccg cggtttccac tccggtgttt gcacccccgt atggtattgc agcacctttc
    19561 cagggaaaca acaccagcac aggagcagga gcgtcagcat cattgcgcca gccagcggtg
    19621 ccatttgggc aataaatccg gcaaacgaaa gaccagaacg tccccagata agaatatttt
    19681 gcgggttgcc aattggcgtc agtagcgaac cagcattgac tgccagcgcc tcaaaaataa
    19741 tcagacgatt aaccgggatc tcacacagtc tttttagcgt gatagtcagc ggaacaacaa
    19801 taaacagcgc gacatcgttg gtcagaaagg tagaaagcag cgccgccgcc agcaccataa
    19861 acatcgccag ccgacgctcc gtagcaaagc ggcgcaccat tttgcgcccc agcacatcaa
    19921 aataaccgct taactccaca cctttggtca gcagcatcag gccgcttaag gtgatgatgg
    19981 tgtgccagtc gatagcagca ggccaggatt tcggtgcaaa gggcacgaaa aagcttaatc
    20041 cgataccaac aagaattaat aactgaaaaa aacgatcgcc ttgcagcgtg cgtaaaaaag
    20101 gcaggctcat ttggtaccgt gtttctgggt aaacaaacga aaagcgtcga gcgtctcttc
    20161 actaacatgg tgctccatgc cttccgcgtc gcgacgggcg atttccggac tgacgcccaa
    20221 caccaacaag aaattttcga ctatctgatg tcgctcgcgg ctttcctgcg ccagcttctc
    20281 tccttctgcc gttaaaaaca cgccacgcca ggggatcatt tcaatcagcc ccatggttgc
    20341 cagccgctta agcattttag ccaccgtcgg ttgcgaaact cccagacgag cagccatgtc
    20401 cacctgacga gcttccccca cttccctgat caagtcagaa atcagctcaa cgtaatcatc
    20461 aataagctcg cgccgatgcg cctctctgac ctggcggaac ccttcaacgt gctcttccac
    20521 gttcactaac tgcgtcactt tttttgctgt tggcgtacct gcgcgacgac tcattgtgct
    20581 tcctcattat ggtgacgcat acatagcgtt ctcatttcgt gcgcacattg taaaccagag
    20641 ttgcgaaggt acaaaaaatt aacgttttag caatagctat ataatatagc ctgtgctata
    20701 tctgtatgta atgcaatcat ccctcaagga tcgacgggat tagcaagtca ggaggtctta
    20761 tgaatgagtt caagaggtgt atgcgcgtgt ttagtcattc tccctttaaa gtacggttaa
    20821 tactgctctc tatgttgtgc gatatggtca acaacaaacc gcagcaagat aaaccttccg
    20881 ataaatagcg gcgtcgcggt acgccgcttc actcctgctt tcatgcaggc ataacgcgtt
    20941 ttggcctgaa aaaccccact ttttgtcgga tttgcaatcc ccttcgcaaa agatttgttc
    21001 gtcagtagtt gacctgaacg gcggctcgct ctatcttctt gcagccctgc gtatattgcg
    21061 gctcgcggat gcggacccct ttccactctt cacgcactct tgcaggtatt gacccttgac
    21121 gccagggtaa gcacatggcg tttgttacga tagtggcata tgaatttaac cctcaaagaa
    21181 tcgcttgtta cccgtagccg ggtatttagc ccgtggactg cgttctactt tttacagtcg
    21241 ctattaatta acctcggctt aggttacccc ttcagtttgc tctacaccgc tgcgtttacg
    21301 gctattttgc ttttgctatg gcgaacattg cctcgcgtac aaaaagttct ggtcggtgtc
    21361 agttcgctgg tggcggcttg ttatttccct tttgctcagg cctacggcgc gcctaatttc
    21421 aatacattgc tggcattgca ctccaccaat atggaagagt cgaccgaaat cctgacgatt
    21481 tttccgtggt acagctacct ggtcggctta tttatttttg cgctcggcgt aatagcaatc
    21541 aggcgaaaaa aagagaatga aaaagcgcgc tggaatacct tcgacagcct gtgtctggta
    21601 ttcagtgtgg cgacattttt tgttgctccc gtgcaaaacc tggcctgggg tggcgtattt
    21661 aaactgaaag atactggcta tccggtattt cgttttgcta aggatgtcat cgtcaataat
    21721 aacgaggtga ttgaagagca agaacggatg gcaaaacttt ccggaatgaa agatacctgg
    21781 acggtcactg ccgttaagcc gaagtatcag acctatgtgg tggtgatcgg tgaaagcgcg
    21841 cgtcgcgatg ccctcggtgc ctttggcggt cactgggaca ataccccgtt tgccagcagc
    21901 gttaacggtt tgatatttgc tgactacatt gccgccagtg gctccacgca gaaatcgctt
    21961 ggcttaacgc tcaatcgcgt agtcgatggc aaaccacagt ttcaggataa ctttgtcacc
    22021 ctggcaaatc gcgcgggctt ccagacctgg tggttttcca accagggcca aatcggcgaa
    22081 tacgatactg ctatcgccag catcgccaaa cgtgcagatg aagtgtactt cctgaaagaa
    22141 ggtaattttg aagcagataa aaacacgaaa gacgaagcgt tactggatat gaccgctcaa
    22201 gtgctggcgc aagagcactc gcaaccgcag ctgattgttc tgcatctgat gggctcgcat
    22261 ccgcaggcct gcgacaggac acaaggcaaa tacgaaacct ttgtgcaatc gaaagaaacg
    22321 tcgtgctatc tctataccat gacgcaaacg gacgatttac tgcgcaagct gtacgatcag
    22381 ttacgcaaca gcggcagcag cttctctctg gtttactttt ctgaccacgg tctggccttt
    22441 aaagagcgcg gcaaagacgt gcaatacctt gcccatgatg ataaatatca gcaaaatttc
    22501 caggtgcctt ttatggtcat ttccagcgac gataaagcgc atcgggtgat taaagcccgc
    22561 cgctcagcca atgacttctt aggctttttc tcgcagtgga cgggaattaa agcgaaggaa
    22621 ataaatatca aatacccgtt tatatctgag aagaaagccg ggccgatata catcaccaac
    22681 ttccagttac agaaggtgga ttacaaccat ctcggaaccg atattttcga cccgaaacct
    22741 taaaaacaaa aatccgcccc gagaggcgga ttttttatat caccaaagtg attagaagcg
    22801 gtaaccaaca ccggcaatcc aggtgcctac gtcaacgcta cgaatacggc tctgctcgta
    22861 agagaagtcc agagcaacgt tttccatcgg gttgaactgc agaccagcac cgtaggagaa
    22921 accgtagtcg ctggtgtcgt gtttgtaggt cgggtattca gtggtctgga atttaccata
    22981 acccacaccc actacaccgt agatgcttgc ccagtcgtta atgcggtaag ccggaccagc
    23041 agtgatgccg tagtactggt ttttgttgta gtcaccagag cttgcagtac gacttttctc
    23101 ggtgtaagtg aaagaaccga tcacacccag cgggctgttg tcttcttcat agcggtattt
    23161 caggttgaaa ccgcccattt tgttcatttg gccctgagcg tcgctctgtg cgtaaccgcc
    23221 agttacggta gaagtcgcag ctacggaagt acctgcggtg aaagccagaa ctgcggccag
    23281 tgctgaaaga catgcaattt ttttcataac cacctcaaat gtgattcaaa taagtcctaa
    23341 gttttaaata tatcaaaaat taatgggaaa ctcttcgcga tttgtgatgt ctaacgggcc
    23401 atttcatgta acagaacgtt tccatacacc gctatccatc taaatttaaa tcactttttc
    23461 agagaactgc gtaagtatta cgcatgtttt ccctgtcatt catccagatt attcctaatc
    23521 accagactaa tgattccatc aatcctggcg cattttagtc aaaacggggg aaaatttttt
    23581 caacaaatgc tcaaccagca ttgggtatat ccagtacact ccacgcttta cttaagtcta
    23641 gatatttgtg ggagaaagga tgcctggttc attacgtaaa atgccggtct ggttaccaat
    23701 agtcatattg ctcgttgcca tggcgtctat tcagggtgga gcctcgttag ctaagtcact
    23761 ttttcctctg gtgggcgcac cgggtgtcac tgcgctgcgt ctggcattag gaacgctgat
    23821 cctcatcgcg ttctttaagc catggcgact gcgctttgcc aaagagcaac ggttaccgct
    23881 gttgttttac ggcgtttcgc tgggtgggat gaattatctt ttttatcttt ctattcagac
    23941 agtaccgctg ggtattgcgg tggcgctgga gttcaccgga ccactggcgg tggcgctgtt
    24001 ctcttctcgt cgcccggtag atttcgtctg ggttgtgctg gcggttcttg gtctgtggtt
    24061 cctgctaccg ctggggcaag acgtttccca tgtcgattta accggctgtg cgctggcact
    24121 gggggccggg gcttgttggg ctatttacat tttaagtggg caacgcgcag gagcggaaca
    24181 tggccctgcg acggtggcaa ttggttcgtt gattgcagcg ttaattttcg tgccaattgg
    24241 agcgcttcag gctggtgaag cactctggca ctggtcggtt attccattgg gtctggctgt
    24301 cgctattctc tcgaccgctc tgccttattc gctggaaatg attgccctca cccgtttgcc
    24361 aacacggaca tttggtacgc tgatgagcat ggaaccggcg ctggctgccg tttccgggat
    24421 gattttcctc ggagaaacac tgacacccat acagctgctg gcgctcggcg ctatcatcgc
    24481 cgcttcaatg gggtctacgc tgacagtacg caaagagagc aaaataaaag aattagacat
    24541 taattaaatt tacatttctg catggttatg cataaccatg cagaatttct cgctactttt
    24601 cctctacacc gtctttatat atcgaattat gcaaaagcat atttattccg aaaattcctg
    24661 gcgagcagat aaataagaat tgttcttatc aatatatcta actcattgaa tctttattag
    24721 ttttgttttt cacgcttgtt accactatta gtgtgatagg aacagccaga atagcggaac
    24781 acatagccgg tgctatactt agtctcgtta attactggga cataacatca agaggatatg
    24841 aaattatgag taccgctaaa ttagttaaat caaaagcgac caatctgctt tatacccgca
    24901 acgatgtctc cgacagcgag aaaaaagcaa cagtagagtt gctgaatcgc caggttatcc
    24961 agtttattga tctttctttg attaccaaac aagcgcactg gaacatgcgc ggcgctaact
    25021 tcattgccgt acatgaaatg ctggatggct tccgcaccgc actgatcgat catctggata
    25081 ccatggcaga acgtgcagtg cagctgggcg gtgtagctct ggggaccact caagttatca
    25141 acagcaaaac cccgctgaaa agttacccgc tggacatcca caacgttcag gatcacctga
    25201 aagaactggc tgaccgttac gcaatcgtcg ctaatgacgt acgcaaagcg attggcgaag
    25261 cgaaagatga cgacaccgca gatatcctga ccgccgcgtc tcgcgacctg gataaattcc
    25321 tgtggtttat cgagtctaac atcgaataaa tccatcgctg atggtgcaga actttagtac
    25381 ccgataaaag cggcttcctg acaggaggcc gttttgtttt gcagcccacc tcaacgcact
    25441 tatttagtgc atccatctgc tatctccagc tgattaagta aattttttgt atccacatca
    25501 tcacacaatc gttacataaa gattgttttt tcatcaggtt ttacgctaaa taatcactgt
    25561 gttgagtgca caattttagc gcaccagatt ggtgccccag aatggtgcat cttcagggta
    25621 ttgccctata aatcgtgcat cacgtttttg ccgcatctct aaaaatcaat gagttgcaaa
    25681 actggcacga ttttttcata tatgtgaatg tcacgcaggg gatcgtcccg tggatagaaa
    25741 aaaggaaatg ctatgaagtc tgtattaaaa gtttcactgg ctgcactgac cctggttttt
    25801 gcggtttctt ctcatgccgc ggataaaaaa ttagttgtcg cgacggatac cgccttcgtt
    25861 ccgtttgaat ttaaacaggg cgataaatat gtgggctttg acgttgatct gtgggctgcc
    25921 atcgctaaag agctgaagct ggattacgaa ctgaagccga tggatttcag tgggatcatt
    25981 ccggcactgc aaaccaaaaa cgtcgatctg gcgctggcgg gcattaccat caccgacgag
    26041 cgtaaaaaag cgatcgattt ctctgacggc tactacaaaa gcggcctgtt agtgatggtg
    26101 aaagctaaca ataacgatgt gaaaagcgtg aaagatctcg acgggaaagt ggttgctgtg
    26161 aagagcggta ctggctccgt tgattacgcg aaagcaaaca tcaaaactaa agatctgcgt
    26221 cagttcccga acatcgataa cgcctatatg gaactgggca ccaaccgcgc agacgccgtt
    26281 ctgcacgata cgccaaacat tctgtacttc atcaaaaccg ccggtaacgg tcagttcaaa
    26341 gcggtaggtg actctctgga agcgcagcaa tacggtattg cgttcccgaa aggtagcgac
    26401 gagctgcgtg acaaagtcaa cggcgcgttg aaaaccctgc gcgagaacgg aacttacaac
    26461 gaaatctaca aaaaatggtt cggtactgaa ccgaaataat aacgctacac ctgtaaagcg
    26521 cactggcagt tccctctccc ctctggggag aggattaggg tgaggggcgc aaacccgctc
    26581 cggggccatt aattaccctg aatttgatta tttacaccac ggtaacagga acaacatatg
    26641 cagtttgact ggagtgccat ctggcctgcc attccgcttc tgattgaagg tgccaaaatg
    26701 accctgtgga tttcggtcct cggtctggct ggcggtctgg taatcggatt gctggcaggt
    26761 tttgcacgca ccttcggtgg ttggatagcc aaccacgtcg cgctggtctt tattgaagtg
    26821 atccgcggca cacctatcgt cgtccaggtg atgtttatct atttcgccct gccgatggcg
    26881 tttaacgact tacgcatcga cccatttact gcggcggtgg tcaccatcat gatcaactcc
    26941 ggcgcgtaca ttgcggaaat cacgcgtggt gcggtgctgt ctattcacaa aggttttcgt
    27001 gaagcaggac tggcgctcgg tctttcacgt tgggaaacca ttcgctacgt cattttaccg
    27061 ctggcactgc gtcgtatgct gccgccgctg ggtaaccagt ggatcatcag cattaaagac
    27121 acctcgctgt ttattgttat cggcgtggcg gaactgaccc gtcaggggca agaaattatt
    27181 gccggtaact tccgcgccct tgagatctgg agcgccgtgg cggtgttcta tctgattatt
    27241 accctggtgc tgagctttat tctgcgtcgt ctggaaagaa ggatgaaaat cctgtgattg
    27301 aatttaaaaa cgtctccaag cactttggcc caacccaggt gctgcacaat atcgatttga
    27361 acattgccca gggcgaagtc gtggtgatta tcgggccgtc cggttccggt aaatcgaccc
    27421 tgctgcgctg catcaacaaa ctggaagaaa tcacctccgg cgatctgatt gtcgatggcc
    27481 tgaaggttaa cgatccgaaa gttgacgagc gcctgattcg ccaggaagca ggtatggtgt
    27541 tccagcagtt ttacctcttc ccgcatctga cggcgctgga aaacgtcatg tttggtccgc
    27601 tacgcgtgcg tggcgcgaac aaagaagagg cggaaaaact ggcgcgtgag ctgctggcga
    27661 aagtcggtct ggcagaacgt gcacatcact acccttccga actttctggt ggtcaacagc
    27721 agcgtgtggc gattgcccgc gcgctggcgg tgaagccgaa aatgatgctg tttgatgaac
    27781 cgacttccgc tcttgacccg gaactgcgcc atgaagtgct gaaggttatg caggatctgg
    27841 ctgaagaagg gatgacgatg gtgatcgtga cccacgaaat cggttttgcc gagaaagtag
    27901 cttcgcggct gatctttatc gacaaaggcc ggattgcgga agatggcaat ccgcaggtgt
    27961 tgatcaagaa cccgccgagc cagcgcttgc aggaattttt gcagcacgtc tcttaataag
    28021 acacattgcc tgatgcgcta cgcttatcag gcctacagga tatctggcaa cttattaaaa
    28081 ttgcatgaac ttgtaggacg gataaggcgt tcacgccgca tccggcaaaa aagcccgcac
    28141 gttgtcagca atctgcttaa tatcccttcc tccctttcac ccgaaaggga ggcacaccag
    28201 attcctctca tttaaaatcg cccc
//
LOCUS       DAZPEB010000028        27675 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZPEB010000028 DAZPEB010000000
VERSION     DAZPEB010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27675
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.028"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(210..419)
                     /gene="fumD"
                     /locus_tag="ACHURJ_001123"
     CDS             complement(210..419)
                     /gene="fumD"
                     /locus_tag="ACHURJ_001123"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFR1280158.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELDKQAMETVINALVK"
     gene            774..968
                     /locus_tag="ACHURJ_001124"
     CDS             774..968
                     /locus_tag="ACHURJ_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1280159.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSLILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            976..2388
                     /gene="pykF"
                     /locus_tag="ACHURJ_001125"
     CDS             976..2388
                     /gene="pykF"
                     /locus_tag="ACHURJ_001125"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFR1280160.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            2699..2935
                     /gene="lpp"
                     /locus_tag="ACHURJ_001126"
     CDS             2699..2935
                     /gene="lpp"
                     /locus_tag="ACHURJ_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFR1280161.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(2999..4003)
                     /gene="ldtE"
                     /locus_tag="ACHURJ_001127"
     CDS             complement(2999..4003)
                     /gene="ldtE"
                     /locus_tag="ACHURJ_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFR1280162.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLLPVVPAGPNNPLGRYALRFAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRARQ
                     "
     gene            complement(4152..4568)
                     /gene="sufE"
                     /locus_tag="ACHURJ_001128"
     CDS             complement(4152..4568)
                     /gene="sufE"
                     /locus_tag="ACHURJ_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFR1280163.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQNQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            complement(4581..5801)
                     /gene="sufS"
                     /locus_tag="ACHURJ_001129"
     CDS             complement(4581..5801)
                     /gene="sufS"
                     /locus_tag="ACHURJ_001129"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFR1280164.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLHTL
                     FDEKTRLLAITHVSNVLGTENPLAGMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSVLGLNNIAEYELNLMHYALSQLESVPNLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            complement(5798..7069)
                     /gene="sufD"
                     /locus_tag="ACHURJ_001130"
     CDS             complement(5798..7069)
                     /gene="sufD"
                     /locus_tag="ACHURJ_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFR1280165.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTSLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMYITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLAEDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            complement(7047..7790)
                     /gene="sufC"
                     /locus_tag="ACHURJ_001131"
     CDS             complement(7047..7790)
                     /gene="sufC"
                     /locus_tag="ACHURJ_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFR1280166.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLAVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQ"
     gene            complement(7800..9287)
                     /gene="sufB"
                     /locus_tag="ACHURJ_001132"
     CDS             complement(7800..9287)
                     /gene="sufB"
                     /locus_tag="ACHURJ_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFR1280167.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            complement(9296..9664)
                     /gene="sufA"
                     /locus_tag="ACHURJ_001133"
     CDS             complement(9296..9664)
                     /gene="sufA"
                     /locus_tag="ACHURJ_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFR1280168.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            complement(10212..10400)
                     /locus_tag="ACHURJ_001134"
     CDS             complement(10212..10400)
                     /locus_tag="ACHURJ_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFR1280169.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            complement(10500..10910)
                     /gene="menI"
                     /locus_tag="ACHURJ_001135"
     CDS             complement(10500..10910)
                     /gene="menI"
                     /locus_tag="ACHURJ_001135"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFR1280170.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            complement(10907..13963)
                     /locus_tag="ACHURJ_001136"
     CDS             complement(10907..13963)
                     /locus_tag="ACHURJ_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFR1280171.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDVLDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDQEMLGLNIVEFAGDDEALIDERVNALCARLDELIASHQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPELDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTL
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLESLNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            14352..15464
                     /gene="ydiK"
                     /locus_tag="ACHURJ_001137"
     CDS             14352..15464
                     /gene="ydiK"
                     /locus_tag="ACHURJ_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFR1280172.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            15650..15757
                     /gene="rprA"
                     /locus_tag="ACHURJ_001138"
     ncRNA           15650..15757
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHURJ_001138"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            15935..16161
                     /locus_tag="ACHURJ_001139"
                     /pseudo
     CDS             15935..16161
                     /locus_tag="ACHURJ_001139"
                     /inference="COORDINATES: protein motif:HMM:NF020535.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1870 family protein"
     gene            16217..16939
                     /locus_tag="ACHURJ_001140"
                     /pseudo
     CDS             16217..16939
                     /locus_tag="ACHURJ_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            16951..17817
                     /gene="ydiB"
                     /locus_tag="ACHURJ_001141"
                     /pseudo
     CDS             16951..17817
                     /gene="ydiB"
                     /locus_tag="ACHURJ_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
     gene            17849..18607
                     /gene="aroD"
                     /locus_tag="ACHURJ_001142"
     CDS             17849..18607
                     /gene="aroD"
                     /locus_tag="ACHURJ_001142"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFR1280173.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVACAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            18753..20348
                     /locus_tag="ACHURJ_001143"
     CDS             18753..20348
                     /locus_tag="ACHURJ_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HFR1280174.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQSQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFNFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEIADGKLNIVQEGRVKKFIQELPEI
                     TFSGKIALERELDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            20362..21513
                     /locus_tag="ACHURJ_001144"
     CDS             20362..21513
                     /locus_tag="ACHURJ_001144"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFR1280175.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFAEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(21556..22467)
                     /locus_tag="ACHURJ_001145"
     CDS             complement(21556..22467)
                     /locus_tag="ACHURJ_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1280176.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYIKKDVLIKDILFYLSNNYREKITLEQLSKKFRASVSYICHEFT
                     KEYRISPINYVIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPS
                     DYRRQFKNCFAEQEILSEFPQPVSLVG"
     gene            22781..23552
                     /locus_tag="ACHURJ_001146"
                     /pseudo
     CDS             22781..23552
                     /locus_tag="ACHURJ_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
     gene            23572..24510
                     /locus_tag="ACHURJ_001147"
     CDS             23572..24510
                     /locus_tag="ACHURJ_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFR1280177.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAAPLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            24566..25855
                     /locus_tag="ACHURJ_001148"
     CDS             24566..25855
                     /locus_tag="ACHURJ_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR1280178.1"
                     /translation="MSDDKFDAIVVGADVASSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILQANVVVLAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQMVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KEREDFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYSRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            25852..26145
                     /locus_tag="ACHURJ_001149"
     CDS             25852..26145
                     /locus_tag="ACHURJ_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFR1280179.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            26202..>27675
                     /gene="fadK"
                     /locus_tag="ACHURJ_001150"
     CDS             26202..>27675
                     /gene="fadK"
                     /locus_tag="ACHURJ_001150"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HFR1280180.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGATYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVSVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSPIIADNTSLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNVLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYCSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSL"
BASE COUNT         6905 a         7002 c         6802 g         6966 t
ORIGIN      
        1 actgatattt attatcattt gaaataaatt taacttaagc gacaattatt tgtgacgttc
       61 attgcagaaa ataaaaataa tattatcgtt ataaattaaa atacataccg caattcgtat
      121 aatttcagca gttgttattt agaatgatta actggaccgc aactgaagca agaatatcag
      181 gatgaagctt accgcctcat cctgaatggt cattttacca gtgcgttaat gacggtttcc
      241 atcgcctgct tatccagctc actgcgctga atgcgtttat tggctaatgc tgtacgtaat
      301 acgcctgcga taacaatatg ctttggttcc tgccccagat cgcgcatttc cagcacaact
      361 ttaccgacaa ctctgcacat ttctcggtac agttcatcct cttttgtccg atttcccatt
      421 ctttatacct atttatcatg ccaactatca gcatatatca atctaaccaa ttaaacaaaa
      481 atcaaacaaa atcagacaaa taacgcgata aattatttta actgtagcaa ttgagcgatg
      541 atatatttat acaccggatg aactttcact tatcctcaca ctgacaactt tggcaccaga
      601 cgttgcgcaa acagcgaagt ttttgcgtaa ccttttccct ggaacgttaa atctttgata
      661 acaatttatt gtctaacaag ttgtatattt tttgaaacgc tgtttttgtt ttccttttgg
      721 attaatttca gcgtataatg cgcgccaatt gactcttgaa tggtttcagc actttggact
      781 gtagaactca acgactcaaa aacaggcact cacgttgggc tgagacacaa gcacacattc
      841 ctctgcacgc tttttcgatg tcacttatcc ttagagcgag gcaccaccac tttcgtaata
      901 ccggattcgc tttccggcag tgcgcccaga aagcaagttt ctcccatcct tctcaactta
      961 aagactaaga ctgtcatgaa aaagaccaaa attgtttgca ccatcggacc gaaaaccgaa
     1021 tctgaagaga tgttagctaa aatgctggac gctggcatga acgttatgcg tctgaacttc
     1081 tctcatggtg actatgcaga acacggtcag cgcattcaga atctgcgcaa cgtgatgagc
     1141 aaaactggta aaaccgccgc tatcctgctt gataccaaag gtccggaaat ccgcaccatg
     1201 aaactggaag gcggtaacga cgtttctctg aaagctggtc agacctttac tttcaccact
     1261 gataaatctg ttatcggcaa cagcgaaatg gttgcggtaa cgtatgaagg tttcactact
     1321 gacctgtcag ttggcaacac cgtactggtt gacgatggtc tgatcggtat ggaagttacc
     1381 gccattgaag gtaacaaagt tatctgtaaa gtgctgaaca acggtgacct gggcgaaaac
     1441 aaaggtgtga acctgcctgg cgtttccatt gctctgccag cactggctga aaaagacaaa
     1501 caggacctga tctttggttg cgaacaaggc gtagactttg ttgctgcttc ctttattcgt
     1561 aagcgttctg acgttatcga aatccgtgag cacctgaaag cgcacggcgg cgaaaacatc
     1621 cacatcatct ccaaaatcga aaaccaggaa ggcctcaaca acttcgacga aatcctcgaa
     1681 gcctctgacg gcatcatggt tgcgcgtggc gacctgggtg tagaaatccc ggtagaagaa
     1741 gttatcttcg cccagaagat gatgatcgaa aaatgtatcc gtgcacgtaa agtcgttatc
     1801 actgcgaccc agatgctgga ttccatgatc aaaaacccac gcccgactcg cgcagaagcc
     1861 ggtgacgttg caaacgccat cctcgacggt actgatgcag tgatgctgtc tggtgaatcc
     1921 gcaaaaggta aatacccgct ggaagcggtt tctatcatgg cgaccatctg cgaacgtacc
     1981 gaccgcgtga tgaacagccg tctcgagttc aacaatgaca accgtaaact gcgcattacc
     2041 gaagcggtat gccgtggtgc cgttgaaact gctgaaaaac tggatgctcc gctgatcgtg
     2101 gttgctactc agggcggtaa atctgctcgc gcagtacgta aatacttccc ggatgccacc
     2161 atcctggcac tgaccaccaa cgaaaaaacg gctcatcagt tggtactgag caaaggcgtt
     2221 gtgccgcagc tggttaaaga gatcacttct actgatgatt tctaccgtct gggtaaagaa
     2281 ctggctctgc agagcggtct ggcacacaaa ggtgacgttg tagttatggt ttctggtgca
     2341 ctggtaccga gcggcactac taacaccgca tctgttcacg tcctgtaata ttgcttttgt
     2401 gaattaattt gtatatcgaa gcgccctgat gggcgctttt tttatttaat cgataaccag
     2461 aagcaataaa aaatcaaatc ggatttcact atataatctc actttatcta agatgaatcc
     2521 gatggaagca tcctgttttc tctcaatttt tttatctaaa acccagcgtt cgatgcttct
     2581 ttgagcgaac gatcaaaaat aagtgccttc ccatcaaaaa aatattctca acataaaaaa
     2641 ctttgtgtaa tacttgtaac gctacatgga gattaactca atctagaggg tattaataat
     2701 gaaagctact aaactggtac tgggcgcggt aatcctgggt tctactctgc tggcaggttg
     2761 ctccagcaac gctaaaatcg atcagctgtc ttctgacgtt cagactctga acgctaaagt
     2821 tgaccagctg agcaacgacg tgaacgcaat gcgttccgac gttcaggctg ctaaagatga
     2881 cgcagctcgt gctaaccagc gtctggacaa catggctact aaataccgca agtaatagta
     2941 cctgtgaagt gaaaaatggc gcacattgtg cgccattttt tttgtctgct atttaccact
     3001 actgcctcgc gcgtaacata ttcccttgct ctggttcacc attctgcgct gactctactg
     3061 aaggcgcatt gctggctgcg ggagttgctc cactgctcac cgaaaccgga taccctgccc
     3121 gacgatacaa cgctttatcg actaacttct gatctacagc cttattgtct ttaaattgcg
     3181 taaagcctgc tggcagtgtg tatggcattg tctgaacgtt ctgctgttct tctgccgata
     3241 gtggtcgatg tacttcaaca taacgcatcc cgttaggctc cacggaatat ttcaccggtt
     3301 cgttgatcac tttcaccggc gttcccgtcc gcacgctgga gaataaggct ttaatatccg
     3361 gtgcattcat gcgaatacag cccgaactga cgcgtaaacc gacgctgtcc ggcgcactgg
     3421 taccatgaat gaggtattcg ccattaccat gcgcgaagcg cagtgcgtaa cgtcctagcg
     3481 ggttatttgg tccggcagga acgactggca gtaatttaat gccacgctcc agcgaacgtt
     3541 gacgaatgcc tgccgtaggc gtccaggttg gattagggat tttctgcccc acacgcgttt
     3601 ccatcaccgg cgtttccagc ccctgcaagc caatacctat cggataaacc tgcacgatat
     3661 tttctcccgg cggataataa taaaggcgca gctctgcaag gttaacgata atcccctgac
     3721 gcggtgcatc aggtaataac agttgtgaag gaatagttat cgtcgtacca ggttttggca
     3781 ccggggcgat agtgttattg gcttcaagga tcaacattgc cgcagtatca aaacgtcggg
     3841 caatagcctg aaggttttta tccccttctt gcaccgtata cgtttgattt tgcccaacca
     3901 gtcggcttcc ggttggtggt agcggataat caaccgccca ggcagcctgg atggcgctaa
     3961 aagcgccgat aagcgtgagt gtaagcaaag acgcgcgttt cattgtaaac ctcctgtatt
     4021 tgccggagac tcacgctgaa acgtcggatg gcgcttatgt tcacctgaaa ccaaaacact
     4081 cctgtgcagg tcagtgtaaa cattgaccat ccggcaatgt gagccaaccg gatgaaagct
     4141 gtccctttag tttagctaag tgcagcggct ttggcgcgaa ttgcgcgaat catcgcttcc
     4201 agaccttgtg aacgagatgg ggtgagatgt tgggtgagcg ccattttttc aaaccacgga
     4261 cgcacatcga aattgacaat atcctgcggc gtcatctgat cgtagagaat aaatacgacc
     4321 gcaataagcc ctttcacaat cgccgcatcg ctgtcgccct gcaattcaat aattccctgc
     4381 gcattctggc gcatgacaat ccacacctga ttctggcagc cctgaatgct attttgtggg
     4441 cttcggtctt cgtcgcgtaa ttctggcaga cgctggccca gctcaataat gtagagatat
     4501 ttctcttccc agttggcgca gcgtaaaaaa ttacgcagca atttttcttt atccggcaat
     4561 aaagccatag tgcctccctg ttatcccagc agacggtgaa tacgttgcag gccggtcacc
     4621 agacgatcca cttcttcatg agtgttatac atggccagcg acgcccgaca catcgcaggg
     4681 acgttgtaat aggccattaa tggcatagcg cagtgatgtc cggtacgcac agcaatgccg
     4741 taattatcga gaaaactgcc aacatcatag gcgtggtgtt taccgagatt aaaagcaata
     4801 acgccaagcc tgttttgtgg gccatagaga gtgagattcg gtacagattc cagctgtgat
     4861 agcgcgtaat gcatcagatt cagttcatac tcggctatgt tattaagccc cagcaccgaa
     4921 acatattcca gcgccgcgcc aagaccaatg atgcccccgg tattgggtgt accggcttca
     4981 aaccgccatg gtgctttggt ccaggtagtg ccttcactca ggctgacggt ggcgatcata
     5041 gaaccgcccc cttcccacgg cggcatctcc tgcaacaagg cttctttgac ataaagaatg
     5101 ccaatcccgg tggggccata cagtttatgc ccggaaaaca cgtaaaaatc gcaatccagc
     5161 gcctgaacat ccaccggatg atgcatcacc gcctgagcgc catccaccag cacttttgcg
     5221 ccatgctggt gcgcaagcgt gatcattccc gccagtggat tttctgtgcc aagcacgttg
     5281 gagacatgag taattgccag caggcgagtt ttctcatcaa acagcgtatg cagcgtctcc
     5341 agttgcagcg taccgtcggg gttgagaggg atcacacgca gctctgcgcc aacgcgtgcg
     5401 caaagcatct gccaggggac aatgttagcg tggtgctcca tctgactgat gatgatgtta
     5461 tcgcccgccc gcacattgct gttgccccag ctattggcga ccagattgat cccttccgtc
     5521 gtgccgcgga cgaacaccag ctcttccgcc gaacgggcat taataaacag cgatgcccgc
     5581 ttgcgcacgt tctccatttt ctcggtcgcc tgggcgctta aggtatgaat accgcgatgc
     5641 accgccgcgt agccatgacg ataaaactcg gcctcggcgt caatcacctg gctcggtttc
     5701 tgtgcactgg cggcgctgtc gagataagcc agcggcaaac cgtttacctc acgcgaaagc
     5761 accggaaagt cggcccgcac tttgtcgacg gaaaaagtca tcttgcacct cctggcagcc
     5821 gttgaccgat tcgggccagc acctgctgtt taagcccctc atcacgcagt gcttccgtca
     5881 gttcggcagc aaaggcgtaa atgatcatct gctgggcatc ctgctgattg atcccgcgcg
     5941 agcgcagata gaacatctgt tcatcatcaa tacgccccac cgtcgcgccg tggctgcatt
     6001 tcacatcatc tgcatagatt tccagctgcg gtttcgtatc cacttccgcc agtttgccca
     6061 tcagcagatt attgttggtc atctgaccat ccgttttgat ggcgtgctgc gcgacgttga
     6121 tcaaaccgtt aaataccgcg cggcctttgt cgctgacgat agttttgtgc aactgtcggc
     6181 tgttacaaaa acctttattg tgttccagcc aggtacgggt atcacacacc tcgtttttca
     6241 ccggcatcgc caggctattg atccgcagcg tgctgttttc gccattgagt tgcgtactgg
     6301 tgttgtgtcg taacactgcg ccacccagca ggaaactatg gctaaatgcg gtggcatcct
     6361 cagccagcaa caaatcgtta tgagcaaagt ggtgactgag cgggttttca aacgccagct
     6421 tgatatgctg caagtgggcg ttcgctgcga cattgatagt gaaccgtgcc ccggtaaaat
     6481 gacgagcatc attaaggctg acaaaatgtt cgatcaccgt tgcttcggca ccttccgcca
     6541 gatccagatg atgccggtaa tgggcagtgt tcacctcttc acctgccacg ccctgggtga
     6601 tatacattaa cagcaatggc tttgccggcc gttgaccgcg cttcacggcg atatgcgtca
     6661 cgctttgtgc caggctttcc gtcaaatgca gaaacacttc cgcctgaata gcgtcgggta
     6721 acccctgacg gtcgtcgtta atgctcactt catatccgct gccttcaatt gcatcgctca
     6781 gcgcgggcac gtaacgtcca tcgacaaaca ccagccgcac ggcgtctaac gttaacgcta
     6841 aggcatcacg ctgctgtggg gatatctccc ccgcaatgct gacaaactgg ctattggtca
     6901 gcccttccag cgacgtatat ttccagtttt catgtttacg tgtcggcagt ccggtacgca
     6961 gcaattgttg taaatgctgc tgtgcttgcg gggagcgttt tgtcccttca gcttcaaaca
     7021 agtgatgcca ctgttgcagc gcgttactac tgttcggtaa gccagccata accctgctcc
     7081 tccagttgtt tgaccaacgt gaaatcgccg gatttcacaa ttcgtccctg atatagcaca
     7141 tggacgtaat caggcttgat gtagtcgaga atgcgttggt agtgcgtaac aatgatgaat
     7201 gagcgcttgc catcacgcag ggagttcacg ccatcggcga ccacttttaa tgcgtcaata
     7261 tccagcccgg agtccgactc atcaagaatg cataactccg gttccagcac cgccatttgc
     7321 aaaatatcgt tgcgcttttt ctcgccgccg gaaaaaccaa cgtttaccga acgggttaat
     7381 aaatcttccg gcatcttcag gagagcgatt ttctcttcca tcaaatcctg aaaatcaaag
     7441 cggtcgagcg tttcctggcc gcgatagctg cgcactgcat taagtgccgt ttgcaggaaa
     7501 aactggttac tgacgcctgg aatctccacc ggatactgga aggccataaa gatgccttcg
     7561 cctgcacgat cttccggcga cagtgcaagc aaatctttgc ctttgaactc aaccgtgccg
     7621 cccgtcactt cataatcttc gcgcccggca agcgttgccg ataaggtact tttgcccgaa
     7681 ccgtttggcc ccataatggc gtgaacttcg ccgggacgaa cggcgaggct taatccacgc
     7741 aggatagctt tatcttccac gctgacgtgt aaatctttaa tacttaacat gtttattcct
     7801 tatccgacgc tgtgttcaag actgatcgcg aggagttttt gtgcttcaac ggcaaattcc
     7861 agcggcagtt ctgagaacac gtctttgcag aaaccgttaa caatcatcga gatggcgtct
     7921 tcttcgctga tcccgcgttg caggcagtaa aacagttgat cttcaccaat acgtgatgtc
     7981 gttgcctcgt gctccagttg tgcgctgtta ttgcgacatt cgacatacgg gaaggtatgc
     8041 gccccacaat tagcgccaat cagcattgag tcgcactgag tgaaattgcg cgcattggtt
     8101 gccgtcggca tgattttcac taagccgcga taactgttct gactatgtcc ggcagagatc
     8161 cctttcgaga taatggtcga tttggtgttt ttaccgatgt ggatcatctt ggtgccggta
     8221 tccgcttgct gatgaccgct ggtcagcgcc actgagtaaa actcaccaat ggagttatcg
     8281 ccgcgcaaaa tgcagctggg atatttccac gtaatcgcgg acccggtttc tgattgcgtc
     8341 catgacattt tgctgttttc gccttcgcac aaagcacgct tggtgacgaa gttgagaata
     8401 ccgccggtgt tgttatcgcc agggaaccag ttttgtaccg tggaatattt cacctcggcg
     8461 tttttatgga tgatgacttc caccactgcc gcgtgtaact gatagctgtc acgcaccgga
     8521 gcggaacagc cttcaatgta gctgacgtag ctgtcttcgt cggccaccag aatggtgcgc
     8581 tcaaactgcc cggttttttc cgcgttaatg cgaaaatagg tggaaagttc catcgggcag
     8641 cgcacgcctt taggcacata aataaacgta ccgtcagagg ctaccgccgc attaagcgcg
     8701 gcaaagaagt tgtcattccc cggcaccacg gtgccgagat atttacgcac cagttccggg
     8761 tgatcgtgga tcgcctcacc aaaggaacag aaaataattc cctgctccgc cagtttttcg
     8821 cgataagtag tggcaaccga aacagagtcg aaaatggcat ccaccgccac ctctttgcct
     8881 tcccgcacgg gaacgcccaa ctgctcaaac gccgcctcca cctctttact taaaaaggcg
     8941 ttcgcgccag tttgctgcac cgcgccaggt tcagacgcgc aagtgtcgtc acaattaccg
     9001 cacgatggtg ctgagtagta gctgtaatcc tgataattca gcttgtcgta gtgcgctttc
     9061 aaccagtgcg gttcttccat ctccagccat gcgcgatagg cgtttagacg aaactccagc
     9121 atccactccg gctcattacg cttcgccgaa attgcgcgca ccacctcttc gtttatcccc
     9181 tttgccagct catcggtggc taactgggtg aagaatcctt ctttataatt cagcgggccg
     9241 ccggtccagg ttttgacatc gtcagttgct tcagtattac gagacatagt accgcctata
     9301 ccccaaagct ttcgccacag ccacattcat tctgggcttt agggttgtga aatttgaata
     9361 tctgattaag tccttcacga acgaaatcga cttccgtgcc atcaataaac ggcatcgctt
     9421 gcagcgggac aaacagcttc gcgccgtcgt gttcaaacag cagatcgtct ttgtccggct
     9481 cgctaacact gtcgagcaca tagccaaagc ccgcgcagcc cgtttgcttc acgcctaaac
     9541 gcacgccgac catacccggc tgctttgcca ccagctcacg gatgtgtatc gccgctgcgg
     9601 gtgtcagcgt taagccttgc caggcgaaat cttgtgggtt aaaggttcct gaatgcatgt
     9661 ccatcgattt acctcacttc atcgctttca gcgtataaca gcatgttggt gataatgatt
     9721 atcagttcaa cccagcaaac gcaggggctt taccgtaaaa catgcttttt gcctgctttt
     9781 aataagcata gaccctgatg tgtgggttaa caggcacgct aagaattagc gtatctcatt
     9841 gttagataat ggttattatc taaggagcat taaggctgtg atgaaaaaga aaaagttgta
     9901 ttaaaatgac tatttaagag ataggtaaaa aagtgcagcg ttcagaaata agaaaaccct
     9961 taagtctgtg cgacacaggc ttaagggttt ctaccccatc cggcgcttat ctccggcact
    10021 ctcagtggct tagctcttga aggggcgata agaataatct cataaagcta acccgccgtt
    10081 ttaacacaaa ctgcgattag tattattttt gaacaatatc aggcggtaga taagcagtat
    10141 taagaaggtc atcgaacctg gacggaggtt aatccaggtc gatttggcga acttgcggca
    10201 ttaagtcagg atcaatgcac gcccagacgc caggcaaagt agatttcttc ttttaattca
    10261 gcagaagaga gagtaagcag gtcagcaaat tcaagttcta gttgtttcag acgtttgaga
    10321 tattgggcag gtgaaagatt gctctggtca cggcgtaaaa attcaatggc cagctgggtg
    10381 ggatcaagtt gagtagacat agcatcctcg cttttagaca agacgtgtac agtataccac
    10441 cgtttactgt gcagataatg accaaaagca atatgcgtca cacttttctg gtgacaacgt
    10501 cacaaaatgg cggtcgtcaa tcgtgacgaa cagcacaaac gccctttctc atcgaagatt
    10561 tcaatctgcc agacctggtg acgcgaaccg agatgcaacg gtttacatat gccgcgcacc
    10621 cgcccttctc gtgccgagcg gacgtgatta gcattgattt ccagaccaac cactttttgc
    10681 tcaccttcgg tacataaata accggcaacg gaaccgatac tttcggccag taccacggat
    10741 gctcctccat gcagcaaccc gaaaggctgc tttgtccgcg agtctactgg cattgtcgct
    10801 tcaagggtgt catcaccaat atgttcaaag cgaatatcca ggaaccccac catgtttcct
    10861 tcacccatag cattcagtgc ttccagggtg attttccgtt tccatatcat ttaataatct
    10921 ccagtaaagc ctgcacagga tggcgtaccc ccgtgccttc aacccgtttt acctggctac
    10981 ggcaggaata tccggtcgcc agacagcggt tacgcggcag tcgctgcata gcctgatgcc
    11041 aggataactc atagatcccg agcgaatttt catggttttt cgcttcatgt ccgtaagtcc
    11101 ctgccatgcc gcagcaaccc acgctgacat tttccagttt cgcgccaaaa cgggcaaata
    11161 tcgcggccca ttgtgctggc gcacccggca aggcggtaac ttcggtacag tgaccaaaga
    11221 aataccatga ttcaccgctg actgtagcca caggctgtga ctcaagtgcg cttgccagcc
    11281 attcattcgc cagtaagacg ttaaactcgc cacgctcctc gcccagcgcc agtttatatt
    11341 catcgcgata acaaagtacc agcgccggat cgacgcccac cattggcata cccagcttcg
    11401 ccatacggtt gaggaaatcc gccgtctttt tcgccgtctt cgcaaaacga ttaagaaaac
    11461 ctttaatatg ctgggctttg ccatttggcg aaaatggcag taacacaggc tggaaaccta
    11521 atttttcgac cagacggaca aaatccgcca ccacttgcgc atcgtaatag ctggtaaagg
    11581 ggtcctgcac caccaacact gtgcgcgctt tctgctctgc attgagagat tcaagctgtt
    11641 ccagcgtcat gtttgccgag cgatgcccca ccatttgttg ttgtagcgag gggaccgaca
    11701 gcagcggcaa atcaaccatg ccgatatgtt tttccgagag tttgcgcacc agcggctggt
    11761 taatgaagaa gttaaaggtt ttcggcgcgc gtgccatcag cggcgcgtag ctctcgaccg
    11821 tagcgacgag gtggtcgcgc agcgggcgta aataacgggt gtgatagagc tgcagaaaac
    11881 gagagcgaaa ctccggcaca tcaattttga tggggcacag ggtcgaacac gctttacagg
    11941 ccagacagcc cgacatcgcc tctttgactt cgtgtgagaa gtcatattcg cctttattcg
    12001 catgccagct attgcgcgtg cgggcaatta acgtccgcaa actgacgccg gattcaggca
    12061 gttcttgttc cagtttgagt ggatcaacgc cgcgatccgc caacaaacgc agccattcac
    12121 gcaccagcgt tgcgcgccct ttcggtgaat gaatccggtt ctgggtgatc ttcatcgacg
    12181 gacacatcgg actacgggca tcaaagttga agcataaacc gttgccgtta cactccatcg
    12241 caccgcgcca ctgctggcgt accgcaatgg ggatctgccg atcgaatgta ccgcgcttca
    12301 ccgcgtccac tttcatcatc ggcgcatcga gaccttctgg cgggcaaatc ttccctgggt
    12361 tgagtcggtt atgcgggtca aatgccgctt tcactttgcg cagttctgca aaaagttcct
    12421 caccgaaaaa cgccgggctg tattcagcgc gaaaaccttt gccgtgctcg ccccacaaca
    12481 aaccaccgta tttcgcagtc agcgccacca cgtcatcaga gatttgcttc atcaaaatct
    12541 cttgttgagg atcgcacata tccagctctg gacggacgtg caagacacct gcgtcgacgt
    12601 gaccgaacat accgtagctt aagccgtggc tgtcgagcag cgcgcgaaat tcagcaatat
    12661 aatccgccag gtgttccggc ggtacgcagg tatcctcagc aaacggaatt ggcttagcgg
    12721 cacctttggc attgccaagc agaccaacgg cttttttgcg cattgcatag atacgttcaa
    12781 cccccgccag ctcacggcac acctgccagc cgatgacacc tgcttgatga ctggcgatca
    12841 gctcatccaa ccgcgcacag agtgcattta cccgctcatc aatcagcgcc tcatcatcac
    12901 cagcaaattc cacaatgttc agcccgagca tctcttggtc aggcacatcg gtaatcaact
    12961 cgctgacgga atgccagaca atatcttccc gcgccagatt cagcactttt gagtccaccg
    13021 tctctaccga aagcgcccgc gcctcaacca taaacggcgc gttacgcagc gcagagtcaa
    13081 aagagtcata tttgacgttc accagacggc gcactttagg caagcgcgta atatccagcc
    13141 gcgcttcggt aataaaggcc agcgtccctt ctgaacccgt cagaatgcgc gtcaggtcga
    13201 actcggtcat ctcatcgtta aagacatgac gcagatcgta accggtaaga aagcggttaa
    13261 gtttggggaa gttgtcgata attaactggc gttgctgacg gcaacgttga taaaccgtgt
    13321 tataaattcg cccgattgtg gtattggatt tacccagcgt ttccgccaat tcgacgggta
    13381 aaggttgcgt atcgagaaca tcgcccccca acaacaccgc gcgtacgcca agtacgtgat
    13441 ctgacgtttt gccatagacc agcgatccct gaccggatgc atcggtattg atcatcccac
    13501 cgagcgttgc ccggttgctg gtcgaaagtt ccggcgcaaa aaagtagccg aacggtttca
    13561 ggtactgatt gagttgatct tttatcaccc cggcctcaac gcgcacccag ccctcttcag
    13621 ggttaatttc gatgatgcgg ttcatatggc gggacatatc aacaataatc ccctggttga
    13681 gcgcctgacc gttagtgccg gtgccgccgc cgcggggggt aaagatcagc gatgaatagc
    13741 gttcctgcgc ggcaagacgg gcgatcagcg ccacatctgc ggttgaacgc ggaaatacca
    13801 ccgcatcggg gagaagttgg taaatactgt tgtcggtcga cattgtcaga cgatcggcat
    13861 aacttgtcgc cgtatcgccg gtaaaacctt gttgctccag ctcttgcaaa aaattaagca
    13921 ccagttgaac gacgccgggt gcctgagaaa tctgtggaat cattatattg cccctttcct
    13981 gcggtctgtg atgtaggtcg atacactatt ctttcaggcg gctgcaatag cgcactgaaa
    14041 ggtgatgttt atttactcta tggatttcga gttgcaggaa ggcggcaagc gagtgaactc
    14101 caggagctta caatagtaag tgactggggt gaacgaacgt agccgcagca catgcaactt
    14161 gaaatacgac gagtaaatcg tttgcgtgtt gcctgagttg ttgtaccaca tttttttcta
    14221 acacgcccat cagaattaag ggcagaatcg gcctgttaaa aaccgctgaa attgctcatc
    14281 attatgcagg tgagtttcgc gtgttcacgt cgcgtcgacg atttgacgca caaaaaaggt
    14341 gaaaagtagt tatggtaaat gttcgtcagc ccagggatgt cgcacaaatt ctgctttcgg
    14401 tgctgttttt agccatcatg attgtggcat gtctgtggat tgttcaaccc tttattctcg
    14461 gctttgcatg ggccggtacg gtggttatcg ccacctggcc ggtattgtta cgtttgcaaa
    14521 agatcatgtt tggccgccgc tctctcgccg ttctggtgat gacgctgtta ttagtgatgg
    14581 tgtttatcat ccctattgct ttgctggtta acagtatcgt cgacggcagc ggcccgctaa
    14641 ttaaagccat ttccagcggc gacatgacgt tacccgatct ggcgtggctt aataccattc
    14701 cggtgattgg cgcgaagctg tatgcaggct ggcacaactt gctggatatg ggggggacgg
    14761 cgatcatggc gaaagtccgc ccttatattg gcaccaccac cacctggttc gttgggcagg
    14821 cggcgcatat cgggcgcttt atggtgcatt gtgcgctgat gcttctcttc agtgccctgc
    14881 tgtactggcg cggtgaacag gtggcacaag gcattcgcca ttttgcaacc cgtctggcag
    14941 gcgttcgcgg agatgccgcc gtactgctgg cggcacaggc tatccgcgcg gtggcgctgg
    15001 gtgtggtggt gacggcgtta gtacaggcag tgcttggcgg tatcggcctc gccgtatccg
    15061 gcgtacctta tgcaacgttg ctaacggtgt taatgatcct ctcctgcctt gtccagcttg
    15121 gcccgttgcc ggtactgatt ccggcgatta tctggctcta ctggactggc gataccacct
    15181 ggggaacggt attgttagtg tggagcggtg tggttggcac gctggataac gtcatccgcc
    15241 caatgttaat tcgcatgggt gccgatttac cgctgatcct gatcctctct ggtgttattg
    15301 gtggtttgat tgctttcggg atgatcggtc tgtttattgg tccggttctg ttagccgttt
    15361 cctggcgtct gtttgccgcg tgggtggaag aagtcccgcc gccgacggac caaccggaag
    15421 aaattctcga agaacttggc gaaatcgaga aaccgaataa gtaatttctc atcaggcggc
    15481 tctgccgcct gattgttaac ccccgctaat tgatatttgc ttgctcttcc ccatattttc
    15541 ctgcttaccg ccattcagct ggtagtacct gtcgcaaatt ctttacagtt tttaaactaa
    15601 tgagacgaat ctgatcgacg caaaaagtcc gtatgcctac tattagctca cggttataaa
    15661 tcaacatatt gatttataag catggaaatc ccctgagtga aacaacgaat tgctgtgtgt
    15721 agtctttgcc catctcccac gatgggcttt tttttaacat ttttccactt cgctacctcg
    15781 cccctcactc tttccaataa tgttgcacaa gatactgtga ctctcttcaa gccgtcagca
    15841 ggctgataat gttatttata ttttattgtt tatttcatgc agccattaca cgatgaacgc
    15901 ttatgaactc caggcactcc gacatatttt tgccatgact attgatgaat gcgcacctgg
    15961 attgcccaga ccggtgacag tgaaagctgg cgtcagtggg aaaatggcaa gtgtgccatt
    16021 cctgatcgtg tagtcgagca actgttggct atgcgtcagc aaagaaaaaa acatcttcat
    16081 gccatcatag agaaaataaa taaccgcatc ggcaacaaca ccatgcgttt tttccccgac
    16141 ttaaccgcat ttcaacgagt ctaccctgat ggcaatttta ttgactggaa gatctatcaa
    16201 tcggtagccg ctgaaggtaa tgctgttgaa aagcaaattc cccagccagt tggtggacgc
    16261 cagcgtagct aatgaattac cgcaaatgaa cagcaaaccg ttagtctggc tggaaggtgt
    16321 ttcatcggta ctgttcggtg tagccgcatt ctcgaccttt tatgtgattg tggtgtggat
    16381 gcccaaatat gcgatggctt ttgctggtat gtcagaagct gaggcattaa aaaccatctc
    16441 ttattacagt atgggctcgt tggtctgtgt ctttattttt gccgcactac tgaaaaaaat
    16501 ggtccggccc atctgggcta atgtatttaa ctctgcactg gcaacaataa cagcagccat
    16561 tatctatctg tacccttctc cactggtgtg taatgccggc gcctttgtta tcggtttctc
    16621 agcagctggc ggcattttac agctcggtgt ttcggtcatg tcagagtttt tcccaaaaag
    16681 caaagccaaa gtcaccagta tttatatgat gatgggtgga ctggctaact ttgttattcc
    16741 gctgattacc ggttatctgt cgaacatcgg ccttcaatat atcattgttc tcgattttac
    16801 tttcgcgctg ctggccctga ttaccgcaat tattgttttt atccgctatt accgcgtttt
    16861 tattattcct gagaatgatg tgcggtttgg cgagcgtaaa tttagcaccc ggttaaacac
    16921 aattaagcat agaggttaaa ggagttaatt atggatgtta ccgcaaaata cgaattgatt
    16981 gggctgatgg cctatcctat ccgccacagt ttatcgcctg aaatgcagaa taaagcctta
    17041 gaaaaagcgg gattgccatt tacctatatg gcctttgaag tggataacga tagttttccc
    17101 ggagcaattg aaggattaaa agctctaaaa atgcgcggaa ctggtgtatc aatgccgaac
    17161 aaacaactgg cttgttaata tgttgatgaa ttaacgccag cagccaaact ggtgggtgcc
    17221 atcaacacca tcgttaatga tgatggctac ctgcgcggct ataacaccga cggcacgggc
    17281 catattcgcg ccattaaaga gagcggtttt gatatcaaag gcaaaacaat ggtgctgtta
    17341 ggggccggag gtgcctcaac ggctattggc gcgcaggggg caattgaagg tttaaaagaa
    17401 attaaactct ttaaccgtcg ggatgagttc ttcgataaag ccctcgcctt cgcgcagcgg
    17461 gttaacgaaa acaccgattg tgtcgtcacg gttaccgatc tcgccgatca gcaagccttt
    17521 gctgaagccc tggcttccgc cgacatttta accaatggca caaaagtggg tatgaaaccc
    17581 cttgagaatg aatcattagt taatgatatc agtctgttac atccgggact tctggtcact
    17641 gaatgcgtgt ataacccgca tatgacgaag ttattgcagc aggcgcaaca agctggttgc
    17701 aaaacgattg atggatacgg catgttgttg tggcaagggg ctgaacagtt cacgttatgg
    17761 actggcaaag atttccctct ggaatatgtt aaacaggtca tggggttcgg tgcctgacag
    17821 gctgaccgcg tgcagaaagg gtaaaaaaat gaaaaccgta actgtaaaag atctcgtcat
    17881 tggtacgggc gcacctaaaa tcatcgtctc gctgatggcg aaagatatcg cccgcgtgaa
    17941 atccgaagct ctcgcctatc gtgaagcgga ctttgatatt ctggaatggc gtgtggacca
    18001 ctttgccgac ctctccaatg tggagtctgt catggcggcg gcaaaaattc tccgcgaaac
    18061 catgccagaa aaaccgctgc tgtttacctt ccgcagtgcc aaagaaggcg gcgagcaggc
    18121 gatttccacc gaggcttata ttgctctcaa tcgtgcagcc atcgacagcg gcctggttga
    18181 tatgatagat ctggagttat ttaccggcga tgatcaggtt aaagaaaccg tcgcctgcgc
    18241 ccacgcgcat gatgtgaaag ttgtcatgtc caaccatgac ttccataaaa cgccggaagc
    18301 cgaagaaatc attgcccgtc tgcgcaaaat gcagtccttc gacgccgata ttcctaagat
    18361 tgcgctgatg ccgcaaagta ccagcgatgt gctgacgttg cttgccgcga ccctggagat
    18421 gcaggagcag tatgccgatc gtccaatcat cacaatgtca atggcaaaaa ctggcgtaat
    18481 ttctcgtctg gctggtgaag tatttggctc ggcggcaact tttggtgcgg taaaaaaagc
    18541 ctctgcgcca gggcaaatct cggtaaatga tttacgcaca gtattaacta ttttacatca
    18601 ggcataagca ataatatttc ggcgggaata tcctcccccg ccgaactaaa aaaaatatat
    18661 tcaaccgtat ttaataaaaa tatttcgtga gtctctgtgc gctaaatatc catctggcgt
    18721 agggaaaatc acacctgaat cagaaattaa caatgaaacc tgtaaaacca cctcgtatta
    18781 atggacgagt gccggtcctg tcggcacagg aagcggtgaa ttatattccc gacgaagcaa
    18841 cactttgtgt gttaggcgct ggcggcggta ttctggaagc cacgacgtta attactgctc
    18901 ttgctgataa atataaacag actcaaacgc cacgtaattt atcgattatt agtccaacag
    18961 ggcttggcga tcgcgccgac cgtggtatta gtccgctggc gcaagaaggt ctggtgaaat
    19021 gggcattatg tggtcactgg ggacaatcgc cgcgtatttc tgaactcgca gaacaaaata
    19081 aaattattgc ttataactat ccacaaggtg tacttacaca aaccttacgc gccgccgcag
    19141 cccaccagcc tggtattatt agtgatattg gcatcgggac atttgtcgat ccacgccagc
    19201 aaggcggcaa actgaatgaa gtcacgaaag aagacctgat taaactggtc gagtttgata
    19261 acaaagaata tctctattac aaagcgattg cgccagatat cgccttcatt cgcgctacca
    19321 cctgcgacag tgaaggctac gccacttttg aagatgaggt gatgtatctc gacgcattgg
    19381 ttattgccca ggcggtgcac aataacggcg gtattgtgat gatgcaggtg cagaaaatgg
    19441 ttaagaaagc cacgctgcat cctaaatctg tccgtattcc gggttatctg gtggatattg
    19501 tggtggtcga tccggatcaa agccaactgt atggcggtgc gccggttaac cgctttattt
    19561 ctggtgactt cacccttgat gacagcacca aacttagcct gcccctaaac caacgtaaat
    19621 tagttgcgag gcgtgcatta ttcgaaatgc gtaaaggcgc ggtggggaat gtcggcgtcg
    19681 gtattgctga cggtattggc ctggtcgccc gggaagaagg ttgtgctgat gactttattc
    19741 tgacggtaga aacaggtccg attggcggaa ttacttcaca ggggatcgcc tttggcgcga
    19801 acgtgaatac ccgtgccatt ctggatatga cgtcccagtt taatttttat cacggtggtg
    19861 gtctggatgt ttgttatttg agttttgctg aagtcgacca gcacggtaac gtcggcgtgc
    19921 ataaattcaa tggcaaaatc atgggtaccg gtggatttat tgatatcagt gccacctcga
    19981 agaaaattat tttctgcggc acgttaaccg cgggcagttt aaaaacagaa attgccgatg
    20041 gcaaattaaa tatcgtccag gaaggacggg tgaagaaatt tattcaggaa ctaccggaaa
    20101 ttacttttag cggaaaaatc gcgctcgaac gggaactgga tgttcgttac atcaccgaac
    20161 gtgctgtatt cacgctgaaa gaagacggcc tgcatttaat cgaaatcgcc cctggcgtcg
    20221 atttacaaaa agatattctc gacaaaatgg atttcacccc agtgatttcg ccagaactca
    20281 aactgatgga cgaaagatta tttatcgatg cggcgatggg ttttgtcctg cctgaagcgg
    20341 ctcattaaca ggagtatcac gatggatttt tctttaactg aagaacaaga actgctgctg
    20401 gccagtattc gcgaactgat tacgactaac tttgcggaag agtatttccg cacctgcgat
    20461 caaaacggga catatccgcg tgagtttatg cgggcgctgg cggataacgg catttctatg
    20521 cttggcgtgc cggaagagtt cggtggcatc cctgcggatt acgtcaccca gatgctggcg
    20581 ctgatggaag tgtcaaaatg cggtgctccg gcatttttga ttaccaacgg tcaatgtatt
    20641 cacagtatgc gccgtttcgg ttctgcagag cagctacgta aaacggcaga gagcaccctg
    20701 gaaacgggtg atcccgccta tgccctggcg ttgacggagc caggcgcagg ctcagacaac
    20761 aacagtgcca ctaccactta cacgcgtaaa aacggcaagg tttacatcaa cggacagaaa
    20821 acctttatta ccggtgcgaa agagtacccg tatatgctgg tgttggcgcg cgatccgcaa
    20881 ccgaaagatc ccaaaaaagc cttcaccctg tggtgggtcg actccagtaa gcccggcatt
    20941 aagattaacc cgctgcataa aatcggctgg catatgctca gcacctgcga agtctatctc
    21001 gacaacgtgg aggttgaaga gagcgacatg gtgggcgaag aaggaatggg tttcctcaat
    21061 gtgatgtaca actttgagat ggagcgcctg atcaacgccg cgcgcagcac cggctttgcc
    21121 gaatgcgcct ttgaagatgc cgcccgctat gccaaccaac gtatcgcttt tggtaagccc
    21181 attggtcata accagatgat ccaggaaaaa ctggcgctga tggcgattaa gatcgacaac
    21241 atgcgcaaca tggtgctgaa agtggcatgg caagccgatc agcatcagtc actgcgcacc
    21301 agcgcggcgc tggcaaaact gtattgcgca cgtaccgcaa tggaagtcat tgatgatgcg
    21361 attcaaatca tgggcggtct gggctatacc gatgaagcgc gcgtctcccg cttctggcgt
    21421 gatgtccgtt gtgaacgtat cggcggcggt acagacgaaa ttatgattta cgtagcaggt
    21481 cggcagatcc tgaaagacta tcagaacaaa taatctgcag gcggcgcagc ttcttaacaa
    21541 actgcgccgc cagatttatc caacaagact taccggttga ggaaattcag ataggatttc
    21601 ttgttccgca aaacagtttt taaattgtct acggtaatcg ctgggcgaac aaccgacatg
    21661 gcgcaaaaac agtttggcaa agtgatcgac attttcataa cccacacgcc aggagatctc
    21721 tgcctgtgat aattcagtat tagtgagtga ccattttgct tccgtcatac gccgttgaat
    21781 aacatagtta ataggggaaa tacgatactc tttggtaaat tcatggcaaa tataactgac
    21841 gctggcacga aattttttcg ataactgttc taaagtgatt ttttcgcgat aattattact
    21901 cagatagaaa agaatatctt ttatcagaac atctttttta atatacccct gctccgaacg
    21961 ataagcattt ttaaaatttt cgtagtaaag aatggctaac gtataggcaa atgcatccca
    22021 taacgaagat gtttggctat ttttactttg cggcaaaatc acacttagct cattaaataa
    22081 ggtttttatg acttcttttc cctgccccgc ggcaattacc ggacaagaat gcggttgcag
    22141 tagctgattt tcctcagccc cctgaaactg aaagccgtac agcgcacagg tacacgtcgt
    22201 tgccggatcg ttaacgtcag aggccaccgc atgcagcctg ccgcgttcta tcaccacaat
    22261 gtcatccgca tgtgcgacat ataacgaaga atcgatggta aaccttgcaa cccctttctt
    22321 gacgtaaatc aactcggttt cattgtcatg aacgtgatgt ccggactccc attttggatc
    22381 atcgctaaat gcaaaacgtg aaagccgtgg cgttttaccg gcgacaaaca gcgtttcact
    22441 ggcgttatca aaacagcgtt gatacatgac aacctcccta ttccatgagc aagcaaaaac
    22501 aatatatgcc ggataaaata cggcgtgtat tgcggttata caaccgcgtt tagcgagagc
    22561 taaacaagat tatttacact gaatgcaaga ttgtacggtc atggaataac tatattgccg
    22621 caaattgcac ttttgtttta gcgatcgcat ttttttgcaa gattgttggc aaggaaaaca
    22681 gcttgctccg tcgaaaaccc ccaccgctat cgcacactat tttcaggcca tttttacctt
    22741 ccatcggaga tggttccgta tgcgactcac aggagaaatc atgaaaataa taacctgctt
    22801 taagctggtg cctgaagaac aggacattgt tgtcactcca gaatacaccc tgaatttcga
    22861 caatgccgac gccaaaatca gccagttcga tctcaatgcc attgaagctg caagccagct
    22921 cgcaacagat gacgatgaga tagccgcgct gaccgttggc ggctcattgt tgcagaactc
    22981 gaaagtgcgc agtgcgcaaa gatgtgctat cccgcgggcc gcacagcctg tatttggtgc
    23041 aggatgcgca acttgaacat gcactgcctc tcgataccgc aaaagcgctg gcggcagcaa
    23101 ttgaaaagat cggcttcgat ttactgatct ttggtgaagg ttccggcgac ctttatgccc
    23161 agcaggttgg cttgctggtc ggagaaattc tgcaacttcc ggtgattaat gcagtgagtg
    23221 ctattcagcg tcagggtaat acactggtga ttgaacgcac gcttgaagat gatgttgaag
    23281 ttattgaact ctctgttcca gccgtgctct gcgtcacctc cgatattaac gtgccacgca
    23341 ttccttcgat gaaagccatt ctcggcgcgg gtaaaaaacc ggtaaatcag tggcaggcaa
    23401 gtgatattga ctggagccag agcgcgccac ttgctgaact ggtcggcatt cgcgtaccgc
    23461 cgcaaacaga acgtaagcac atcattatcg ataacgattc gccggaggcc attgccgagc
    23521 tggcggaaca tctgaagaaa gccctgaact gaagcctacg gagaagaaac gatgagtcaa
    23581 ttaaacagcg tctgggtctt tagcgataat cctgaacgtt atgctgaact gtttggcggc
    23641 gctcagcaat ggggccaaca ggtgtatgcc attgtacaaa ataccgacca ggcgcaggca
    23701 gttatgcctt atggtccaaa atgtatttat gttcttgagc aaaacgacgc gctgcaacgc
    23761 actgaaaatt acgccgaaag cattgctgcc ccgctgaaag ataaacaccc cgctatgttg
    23821 ctgttggccg cgacgaaacg tggtaaagct ctggcagcac ggttaagtgt gcaactgaat
    23881 gcggcgctgg tgaacgatgc cacggcggtg gatattgtcg atggtcacat ttgcgccgaa
    23941 caccggatgt atggcgggtt agcgttcgct caggaaaaga tcaacagccc gctggcgatc
    24001 attacccttg cacccggtgt tcaggaaccg tgcaccagtg atacctctca tcagtgcccg
    24061 acagaaacgg taccttatgt tgctccgcgt catgaaattc tctgtcgcga acgccgtgcg
    24121 aaagccgcaa gcagcgtgga cctgagcaaa gcaaaacgtg tggttggcgt cggtcgtggt
    24181 ctggcggcgc aggatgacct gaaaatggtc cacgaactgg cggcggtgct gaatgctgaa
    24241 gtcggctgtt cacgtccaat tgccgaaggc gagaactgga tggagcgtga acgttatatc
    24301 ggtgtctccg gcgtgttgct gaaatccgat ctctacctga cgctggggat ctccgggcag
    24361 atccagcata tggttggcgg caacggcgca aaagtgattg tcgccatcaa taaagataaa
    24421 aatgcgccaa tcttcaatta tgccgactac ggtctggtgg gcgatatcta caaagtcgtc
    24481 cctgccctga ttagccagtt gagccgctaa ttcccctttc aaccacatct cccgctgtaa
    24541 acgcagcggg aagggagcat aacgcatgtc ggatgacaaa tttgatgcca ttgtggtcgg
    24601 tgcggacgtt gctagtagcg ttgccgcact ggtcatggcg cgagccggac tggatgtcct
    24661 ggtgatagaa cgcggcgaca gtgccggatg taaaaacatg accggcgggc gtctttatgc
    24721 ccacacactt gaagcaatca ttccaggctt tgcagtatca gcgccggtag aacgcaaggt
    24781 cacacgcgag aaaatctcct tcttaaccga agaaagcgcc gttaccctcg attttcaccg
    24841 cgagcaacca gatgttccgc aacacgcatc ttataccgta ttgcgtaatc gtctggaccc
    24901 gtggttgatg gaacaagccg agcaggcggg cgcacagttt atcccgggcg ttcgcgtcga
    24961 tgcgttggtt cgtgaaggaa acaaggtcac tggcgtgcag gccggggatg atattctcca
    25021 agcgaatgtg gtggttctag ctgatggcgt aaactcgatg cttggccgct cgctgggaat
    25081 ggttcccgct tccgatccgc atcattacgc tgttggtgtt aaagaggtta ttggcctcac
    25141 accagaacag atcaacgatc gctttaatat tacgggcgag gaaggtgccg cctggctgtt
    25201 tgccggctcc ccttctgacg gcctgatggg cgggggattt ctctatacca acaaggattc
    25261 catatccttg gggctggttt gtggattggg tgatatcgcc catgcgcaaa aaagcgtgcc
    25321 gcaaatgctg gaagatttta aacaacaccc cgccattcgc ccactaatta gcggcggcaa
    25381 actgcttgaa tattccgcgc atatggtgcc agaaggcggt ctggcaatgg tgccgcagat
    25441 ggttaacgat ggcgtgatga tcgttggtga cgccgcaggc ttctgcctga atttgggttt
    25501 tacagttcgt ggcatggatt tagccattgc atcggctcag gctgccgcca caacggtgat
    25561 cgccgccaaa gaacgcgagg atttctccgc cagcagtctg gcgcaataca aacgtgagct
    25621 ggaacaaagc tgcgtcatgc gcgatatgca gcatttccgc aagatcccgg cgctgatgga
    25681 aaacccgcgc ctgtttagcc aatactcacg aatggtcgcc gacatcatga acgagatgtt
    25741 caccattgac ggcaaaccaa accagccggt acgcaaaatg atcatgggac acgcgaagaa
    25801 aattgggctg atcaacttgc tgaaagatgg cattaaggga gcaaccgcgc tatgagccag
    25861 aacgctacgg ttaacgtcga catcaaatta ggcgtcaata aattccatgt tgatgagggc
    25921 cacccgcata tcattctggc ggcaaatccc gatatcaatg aattccgtaa attaatgaaa
    25981 gcttgccctg ccggacttta taagcaggat gacgccggca acattcattt cgattccgcc
    26041 ggttgtctgg agtgcggcac ctgtcgggtg ctgtgcggca acactattct cgaacagtgg
    26101 caatatcccg caggcacctt cggtattgat tttcgctacg gctaatcatg catcccacag
    26161 gcccgcatct cgggcctgat gttctgtttc gagagtccaa aatgaaagtg acattaacgt
    26221 tcaacgaaca acgtcgtgcg gcgtatcgtc agcaagggtt atggggcgat gcttcactgg
    26281 ccgattactg gcagcagacc gctcgtgcga tgccagacaa aattgccgtg gtcgacaatc
    26341 atggtgcaac gtacacctat agcgcgctcg atcacgccgc gagctgtctg gcaaactgga
    26401 tgttagcgaa gggtattgaa tcaggcgatc gcatcgcatt tcaactgcct ggctggtgtg
    26461 aatttaccgt tatctatctt gcctgcctga aaatcggtgc ggtttccgtg ccgctgttgc
    26521 cttcctggcg ggaagcagaa ctggtgtcgg tgctcaataa gtgtcaggca aaaatgttct
    26581 ttgcaccgac gttgtttaaa caaacacgtc cggtagattt aatcctgccg ctgcaaaatc
    26641 agcttccaca actacaacaa attgtcggcg tggacaaact ggctcccgcc acctcttccc
    26701 tctcattaag tccgattatc gccgacaata cctcactgac cacggcgata acgacccacg
    26761 gcgatgaatt agctgcggtg ctgtttacct ccggaaccga gggtctgcca aagggcgtga
    26821 tgctaacgca taacaatatt ctcgccagtg agcgggctta ttgcgcgcga ctgaatctga
    26881 cctggcagga tgtctttatg atgcctgcgc cacttggtca cgcaacgggc tttctgcatg
    26941 gcgtaacggc accattctta attggcgctc gcagcgtgtt gttagatatt ttcactcctg
    27001 atgcgtgtct cgcgctgctt gagcagcagc gttgcacctg tatgctaggc gcaacgccgt
    27061 ttgtctatga tcttttgaat gtactagaga aacaacccgc ggacctttca gcgctgcgtt
    27121 tctttctttg cggcggtacc acaatcccca aaaaagtggc acgtgaatgc cagcagcgcg
    27181 gcattaaatt attaagtgtt tatggttcca cagaaagttc gccgcatgcg gtggtgaatc
    27241 tcgatgatcc tttgtcgcgc tttatgcaca ccgatggtta cgctgccgca ggtgtagaga
    27301 ttaaagtggt cgatgacgca cgcaagacct taccgccagg ttgcgaaggt gaagaagcct
    27361 cgcgtggccc caatgtgttt atggggtatt ttgatgaacc tgaattaacc gcccgtgcgc
    27421 tggacgaaga aggctggtat tgcagcggcg atctctgccg tatggatgag gctggctata
    27481 taaaaattac cggacgcaaa aaagatatta ttgtccgcgg cggcgaaaat attagcagcc
    27541 gtgaagtgga agatatttta ttgcagcatc ctaaaattca cgatgcctgt gtggttgcaa
    27601 tgcccgatga acgtttaggt gaacgatcat gcgcttatgt cgtgctgaaa gcgccgcatc
    27661 attcattatc gctgg
//
LOCUS       DAZPEB010000029        27664 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZPEB010000029 DAZPEB010000000
VERSION     DAZPEB010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27664
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.029"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..605
                     /locus_tag="ACHURJ_001151"
     CDS             <1..605
                     /locus_tag="ACHURJ_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1280181.1"
                     /translation="IFKTAIASGLAEEDPASATRPFRTEVKRSRLLIDEYLLIRKIAD
                     VQNEWFGLCMDLALVTGQREGDLAAMRWEDIRDGRLYVEQQKTGAKIRISLPTTISRL
                     NLTLTDVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFHEIRSL
                     SGRLHSAEKGSDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            841..1659
                     /gene="ybhA"
                     /locus_tag="ACHURJ_001152"
     CDS             841..1659
                     /gene="ybhA"
                     /locus_tag="ACHURJ_001152"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFR1280182.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEILNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDMPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1660..2718)
                     /gene="modC"
                     /locus_tag="ACHURJ_001153"
     CDS             complement(1660..2718)
                     /gene="modC"
                     /locus_tag="ACHURJ_001153"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFR1280183.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2721..3410)
                     /gene="modB"
                     /locus_tag="ACHURJ_001154"
     CDS             complement(2721..3410)
                     /gene="modB"
                     /locus_tag="ACHURJ_001154"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFR1280184.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3410..4183)
                     /gene="modA"
                     /locus_tag="ACHURJ_001155"
     CDS             complement(3410..4183)
                     /gene="modA"
                     /locus_tag="ACHURJ_001155"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR1280185.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFIIDSTTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVVSKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4349..4498)
                     /gene="acrZ"
                     /locus_tag="ACHURJ_001156"
     CDS             complement(4349..4498)
                     /gene="acrZ"
                     /locus_tag="ACHURJ_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFR1280186.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4627..5415
                     /gene="modE"
                     /locus_tag="ACHURJ_001157"
     CDS             4627..5415
                     /gene="modE"
                     /locus_tag="ACHURJ_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFR1280187.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNANNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5483..6955
                     /gene="modF"
                     /locus_tag="ACHURJ_001158"
     CDS             5483..6955
                     /gene="modF"
                     /locus_tag="ACHURJ_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFR1280188.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKILLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQHLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7216..8232
                     /gene="galE"
                     /locus_tag="ACHURJ_001159"
     CDS             7216..8232
                     /gene="galE"
                     /locus_tag="ACHURJ_001159"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFR1280189.1"
                     /translation="MRVLLTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGIGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8242..9288
                     /gene="galT"
                     /locus_tag="ACHURJ_001160"
     CDS             8242..9288
                     /gene="galT"
                     /locus_tag="ACHURJ_001160"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFR1280190.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNHDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9292..10440
                     /gene="galK"
                     /locus_tag="ACHURJ_001161"
     CDS             9292..10440
                     /gene="galK"
                     /locus_tag="ACHURJ_001161"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFR1280191.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLAEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10434..11474
                     /gene="galM"
                     /locus_tag="ACHURJ_001162"
     CDS             10434..11474
                     /gene="galM"
                     /locus_tag="ACHURJ_001162"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFR1280192.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11676..12428
                     /gene="gpmA"
                     /locus_tag="ACHURJ_001163"
     CDS             11676..12428
                     /gene="gpmA"
                     /locus_tag="ACHURJ_001163"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFR1280193.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12586..13638)
                     /gene="aroG"
                     /locus_tag="ACHURJ_001164"
     CDS             complement(12586..13638)
                     /gene="aroG"
                     /locus_tag="ACHURJ_001164"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFR1280194.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            13954..14334
                     /locus_tag="ACHURJ_001165"
     CDS             13954..14334
                     /locus_tag="ACHURJ_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFR1280195.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAATDAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14448..15389
                     /gene="zitB"
                     /locus_tag="ACHURJ_001166"
     CDS             14448..15389
                     /gene="zitB"
                     /locus_tag="ACHURJ_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFR1280196.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSIVVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHAHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(15386..16105)
                     /gene="pnuC"
                     /locus_tag="ACHURJ_001167"
     CDS             complement(15386..16105)
                     /gene="pnuC"
                     /locus_tag="ACHURJ_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFR1280197.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMLLMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSCALSH"
     gene            complement(16143..17186)
                     /gene="nadA"
                     /locus_tag="ACHURJ_001168"
     CDS             complement(16143..17186)
                     /gene="nadA"
                     /locus_tag="ACHURJ_001168"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFR1280198.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(17435..17510)
                     /locus_tag="ACHURJ_001169"
     tRNA            complement(17435..17510)
                     /locus_tag="ACHURJ_001169"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17475..17477),aa:Lys,seq:ttt)
     gene            complement(17657..17732)
                     /locus_tag="ACHURJ_001170"
     tRNA            complement(17657..17732)
                     /locus_tag="ACHURJ_001170"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17697..17699),aa:Lys,seq:ttt)
     gene            complement(17736..17811)
                     /locus_tag="ACHURJ_001171"
     tRNA            complement(17736..17811)
                     /locus_tag="ACHURJ_001171"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17776..17778),aa:Val,seq:tac)
     gene            complement(17863..17938)
                     /locus_tag="ACHURJ_001172"
     tRNA            complement(17863..17938)
                     /locus_tag="ACHURJ_001172"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17903..17905),aa:Lys,seq:ttt)
     gene            complement(17941..18016)
                     /locus_tag="ACHURJ_001173"
     tRNA            complement(17941..18016)
                     /locus_tag="ACHURJ_001173"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17981..17983),aa:Val,seq:tac)
     gene            complement(18052..18127)
                     /locus_tag="ACHURJ_001174"
     tRNA            complement(18052..18127)
                     /locus_tag="ACHURJ_001174"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18092..18094),aa:Lys,seq:ttt)
     gene            complement(18292..19083)
                     /gene="cpoB"
                     /locus_tag="ACHURJ_001175"
     CDS             complement(18292..19083)
                     /gene="cpoB"
                     /locus_tag="ACHURJ_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFR1280199.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(19093..19614)
                     /gene="pal"
                     /locus_tag="ACHURJ_001176"
     CDS             complement(19093..19614)
                     /gene="pal"
                     /locus_tag="ACHURJ_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFR1280200.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(19649..20941)
                     /gene="tolB"
                     /locus_tag="ACHURJ_001177"
     CDS             complement(19649..20941)
                     /gene="tolB"
                     /locus_tag="ACHURJ_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFR1280201.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPSAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(21074..22255)
                     /gene="tolA"
                     /locus_tag="ACHURJ_001178"
     CDS             complement(21074..22255)
                     /gene="tolA"
                     /locus_tag="ACHURJ_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFR1280202.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEKAK
                     AEAEKKAAAEKAAADKKAAADKKAAEKAAAEKAAADKKAAAEKAAAEADDIFGELSSG
                     KNAPKTGGGAKGNNASPAGSGNSKNNGASGADINNYAGQIKSAIESKFYDASSYAGKT
                     CTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKPPSQAVYEVFKNAPLDFK
                     P"
     gene            complement(22320..22748)
                     /gene="tolR"
                     /locus_tag="ACHURJ_001179"
     CDS             complement(22320..22748)
                     /gene="tolR"
                     /locus_tag="ACHURJ_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFR1280203.1"
                     /translation="MARARGRGRRELKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(22752..23444)
                     /gene="tolQ"
                     /locus_tag="ACHURJ_001180"
     CDS             complement(22752..23444)
                     /gene="tolQ"
                     /locus_tag="ACHURJ_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFR1280204.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(23441..23845)
                     /gene="ybgC"
                     /locus_tag="ACHURJ_001181"
     CDS             complement(23441..23845)
                     /gene="ybgC"
                     /locus_tag="ACHURJ_001181"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFR1280205.1"
                     /translation="MNTTLFRWPARVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(23995..24288)
                     /gene="ybgE"
                     /locus_tag="ACHURJ_001182"
     CDS             complement(23995..24288)
                     /gene="ybgE"
                     /locus_tag="ACHURJ_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFR1280206.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIIGLIFFFF"
     gene            complement(24288..24401)
                     /gene="cydX"
                     /locus_tag="ACHURJ_001183"
     CDS             complement(24288..24401)
                     /gene="cydX"
                     /locus_tag="ACHURJ_001183"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFR1280207.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(24416..25555)
                     /gene="cydB"
                     /locus_tag="ACHURJ_001184"
     CDS             complement(24416..25555)
                     /gene="cydB"
                     /locus_tag="ACHURJ_001184"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFR1280208.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(25571..27139)
                     /gene="cydA"
                     /locus_tag="ACHURJ_001185"
     CDS             complement(25571..27139)
                     /gene="cydA"
                     /locus_tag="ACHURJ_001185"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFR1280209.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT         6989 a         7280 c         6780 g         6615 t
ORIGIN      
        1 acatttttaa aacagctata gcttctggtc ttgccgaaga agatccagct tctgcaacca
       61 ggccattcag aactgaagtg aagcgcagtc gactgttaat tgacgagtat ctgttaatca
      121 ggaaaatagc agacgtccag aacgaatggt tcggcctttg catggatctt gcactggtaa
      181 caggacagcg cgaaggagat ctggcggcta tgagatggga ggatataaga gatggcagac
      241 tatacgtgga acaacaaaaa acaggggcaa aaattcgcat ttctctcccc accacgattt
      301 ccagattgaa tttaacactt accgatgtac tggataactt gaaaaaaata aacggtaaaa
      361 acgaaaagtt actcggaggg aagacagcca ggacaatcgc agcacagttc aggattgccc
      421 gagacacatc cggattgaaa tgggaaggtg acccaccgcc atttcatgaa atccgaagtt
      481 tatccggtcg cctgcactca gcagaaaaag gcagtgattt cactcaggcg ctccttggac
      541 atcgttcatc cagcatgacg gataaatacc gtgacggacg agggagggaa tggaaagaca
      601 tttagaatta atttcgaatg atttcgaaac accacttgta attaattgat tataaaggat
      661 taattcctaa gaggcatttg cttcatgaat gctcctttgc atttagcaac tgactgtaac
      721 agctaaaatt agtcgctttt ggcggtgaat gctgaattaa aacgcagaat tttgtccgat
      781 agtggtatcg gtgtaccatt cgcgcagacg aaaattctta accttaatct ggaatacgcc
      841 atgaccacac gcgtgattgc tctcgactta gacggcacct tattgacccc gaaaaagacc
      901 ctgcttcctt catcgataga agccctggcc cgcgctcgcg aagcaggcta tcaattaatc
      961 atcgtcacag gtcgccatca cgtcgctatt catccttttt atcaggcgct ggcgctggat
     1021 acacctgcta tttgctgtaa tggcacctat ttgtatgatt atcatgcaaa aaccgtgctg
     1081 gaagcggacc caatgcccgt taataaagcc ctgcaactca ttgagatact gaatgaacac
     1141 cacattcacg gtctgatgta tgtggatgat gcaatggtct atgagcaccc gaccgggcat
     1201 gtcattcgca cgtctaactg ggcgcaaacc ctgccgccgg aacagcgtcc gactttcaca
     1261 caagtcgctt ctctggctga aacggcgcaa caagttaacg ccgtatggaa gttcgccctg
     1321 acgcacgatg acatgccgca attgcagcat tttggtaagc atgtcgaaca tgaactggga
     1381 ctggagtgtg aatggtcctg gcacgatcag gttgatattg cacgcggcgg caacagcaaa
     1441 ggtaaacgtt tgacgaaatg ggttgaggcg caaggctggt cgatggaaaa cgtcgtggcg
     1501 ttcggcgata actttaatga tatcagtatg ctggaggccg ctggtacagg cgtggcgatg
     1561 ggcaacgccg atgacgcggt aaaagcgcgc gccaacattg tgattggtga taacaccacc
     1621 gacagcattg cccagttcat ttatagccac ctgatttaat caggcggtta tcgacacact
     1681 tttaatttgc gcgtacagcc acaggcctgg tttgatcgcc agttcatccc tggcccacgg
     1741 gctgatacgc gcccacagcg ttttaccgcc gacttccagc tccacttcca cctgaccgtt
     1801 gtcgtcataa ctattaacaa cttttgcccg caatacgtta cgaatgctgg tttgctgcgg
     1861 cggttgtaaa accagagaaa catcggaagc ctgaatgcgg atacgtagcg cagcttgcag
     1921 cggttcgtcc agcttattga cccacaaatg ctgatcgccc agcgccagcg cggtcatcgc
     1981 gtaatgcgga tgatgctcaa gcaccgtcac tttcagaatg ctactttgtt gctctttcgg
     2041 cagccacgga ttcatcacgc tactacccca gacttcttcc agtgcgccaa aggctttcac
     2101 ctgaccgttt tccagtacca tcactctgtc tgccagatgg aggatctcat ccagcgaatg
     2161 gctgacatac aacatcggga tgttgatttc ccgcgtcaga cgttgcagat aaggcaacag
     2221 ttcgcgttta cgcggaatat ccagtgacgc cagcggttca tccagcaaca gcaattctgg
     2281 tgccgtcagc aaagcccgcc caatcgctac gcgctgtttt tcgcctccgg acaggctgcc
     2341 tggtaaacgg tcaagcaacg gttcaatgcc taaaagcgcc accagcttat cgaactgatc
     2401 gaccatgctt ttcgccatgc cgtagcgcag attgccacgc actttgtaat gcggaaacag
     2461 ccgcgcatcc tgaaaaacat agccaacgcg acgcttttct ggcgtcaggc agataccttt
     2521 ttcggcatca tttagtaccc gaccattgag gacaatcctc cctttttgcg ggcgcgtcag
     2581 tccactgatg gcgttaatca gcgaagtttt tccggcaccg gagacgccaa agatagcagt
     2641 gatgccattg gcgggcagcg tttcattaat agtcaggcaa tggttgccca acgtctggga
     2701 aaaattcagt tccagcatga ttagcgcccc gcccgttcac ggctgattct ggccagccat
     2761 tctgaaatca acagggagat catcgccagc gcaatagaaa taatgcacag tctcgccgcg
     2821 ccactttccc cgccgggggt ctggatcagg gtatacatgg cagagggaat ggttcgcgtt
     2881 tcgccaggaa tgttcgacac aaaggtgatg gttgcaccaa actcaccgag agaacgagca
     2941 aaagccagta ccgtaccaac aataattccc ggtaaggtca gcggtaacgt gatagtaaag
     3001 aaaacgcgcc agcgcccggc acctagtgtt cttgcggcct gttcgagttt gacatcaacc
     3061 ccttccagcg ccagacgaat tgcccgcacc atcagcggaa acgacatgac ggcggcagcg
     3121 agaaccgcgc cgcgccagct aaaggcgaag gtaataccaa accagtcata cagacgttca
     3181 ccgataaatc cgcgccgtcc catcgaaact aataataagt aaccgacgac cacgggcggt
     3241 aacaccagcg gtagatgcag tacgctgtcg agcagagctt tgcccggaaa cgtgcaacgc
     3301 accagtaacc aggcaaaaaa gatcccaaac ggcaggctaa acagcacagc cagggaagaa
     3361 actttcaggc ttaataaaac tgcctgccat tctggatcgg tcagtatcat tacttggttg
     3421 taaatccgta acgtttaaag atttccgctg cctgcggtcc cttcagataa tcataaaatg
     3481 ctttcactgt cgcattgtta tgcccttcca caacagcaac cggatattcc acttttttat
     3541 gtgaatcttc cgggaaggtg gcaaccactt ttaccccttt gctgacaact gcgtcagaac
     3601 cgtaaacaat gcccaacggc gcttcattgc gttcgaccaa cgccagcgcg ccacgaacat
     3661 cttccgccgg agccaatttc ggggagagcg tatcccatgc gcccagtttt tgcagtgctt
     3721 cttttgcata aatgccagca gggacatgtt ccggatcgcc aaccgcgagg cgaccgccat
     3781 tcagcagcga agtccagtta gttgtgctgt cgatgatgaa atctttctgc tcgctggctt
     3841 tcggtgctac aacgaccagg ctattgccga gcagtgtctg gcgcgaagcc gtatcgatcg
     3901 cttttttatc aaccgcataa tccatccatt tctgatcggc agagataaac agatccgcag
     3961 gcgctcccgc ttcaatctga cgggccagag tagatgacga agcgaaagaa gaaaccacat
     4021 ccacgccttt ttctttttta tactgcgtag caatgtcctg cattgcgtta gtcagtgatg
     4081 cggcggcgaa caccgtgatt ttcccttcat ctgccagtgt attgccagca acagcgaaag
     4141 agagtgccgc cccggcaaac aagttcaacc atttacgagc cattggtaat cccttaatgt
     4201 aacgttatat agatgacaat ataacggcta actcagaatt ttcccagcag ttattgctaa
     4261 cctttgtgag gtagataaga aaaaatatcg gctggaaaag caggaagttg agaaaaagaa
     4321 atgcccgact aagtgggcat tcagggaatc aatgattttg tccgggctgg tcttttttac
     4381 caacaccaga aaagatgttg aatacttcac caagaccgta aatcagaccc aggatgatgg
     4441 ccatcacgac aggtaccatg attacggcga ataccagact ttttaataac tctaacatgg
     4501 tcaactccag atatagtcat gagactattc taaccgctaa gcacagaaaa gcactcccct
     4561 tttgtgcggt cagctttgcg tggcgttcgt tttccgtcac aataagactt ttgccaggac
     4621 attgttatgc aggccgaaat ccttctcacc cttaagctcc aacaaaaatt attcgccgac
     4681 ccgcgccgga tttcgctact aaaacacatt gcgctttccg gttccattag ccagggagcg
     4741 aaagatgccg gtatcagcta taaaagcgcc tgggatgcca ttaacgagat gaatcagtta
     4801 agcgagcata ttctggttga gcgcgcaaca ggcggtaaag gtggcggcgg cgcagtactg
     4861 acccgctatg gtcagcgact gattcagctc tatgacttac tggcgcaaat ccagcaaaaa
     4921 gcctttgatg tgttaagtga cgatgacgcc ctgccgctga acagcctgct ggccgcgatc
     4981 tcacgttttt cactgcaaac cagcgcccgt aaccagtggt tcggtaccat caccgcccgc
     5041 gatcatgatg acgttcaaca gcatgttgat gtcttactgg ctgacggaaa aacacgcctg
     5101 aaagtcgcaa ttaccgcaca aagcggcgca cgtctggggc tggatgaagg caaagaagtg
     5161 ttgatattgc taaaagcgcc gtgggtaggc attactcagg acgaagcggt cgcacaaaac
     5221 gccaacaacc aattaccggg tattattagt catattgagc gcggcgcaga gcagtgcgaa
     5281 gtattaatgg cgctacccga cgggcaaaca ctgtgcgcca cagtgccggt aaatgaagcg
     5341 acttctcttc agcaagggca gaatgtcacg gcctacttta atgccgacag cgtgattatc
     5401 gccacgctgt gctaagcgtg ttgacaattt gttatgaaac acgtatccct gtcagtaatc
     5461 gctgcacaaa atgggatata aaatgtcatc gttgcaaatt ttgcaaggca cgtttcgtct
     5521 tagcgacaca aaaacgctgc aattgcctca gctaacgtta aacgcgggtg atagttgggc
     5581 gtttgtcggt tcgaatggaa gcgggaaatc ggccctggcc cgcgcgctgg cgggggaact
     5641 tccgcttttg aaaggtgaac ggcaaagcca gttttcccac atcactcgtc tctccttcga
     5701 gcaattgcaa aaactcgtca gcgacgaatg gcagcggaat aacaccgata tgctcggccc
     5761 tggcgaagat gacaccggac gcactacggc tgagatcatt caggatgaag taaaggatgc
     5821 accgcgttgc atgcaactgg cgcagcagtt cggtattacc gccctcctcg accgacgctt
     5881 taaatacctt tccactggcg agacgcgaaa aatcctgctg tgtcaggcgc tgatgtcgga
     5941 gcctgacttg ttgattcttg atgagccgtt cgatggcctg gatgttgcct cacgtcagca
     6001 gctggctgag cgactcgcct cgttacatca gtccggtatt actctggtac tggtgctcaa
     6061 tcgcttcgat gagatcccgg agtttgtcca gtttgctggc gtgctggcgg attgcacgtt
     6121 agcggaaact ggcgctaaag aggaactgct ccaacaagca ctcgtcgcgc aactggcaca
     6181 tagtgaacag cttgaaggtg tgcaactgcc ggagccggat gaaccttcag cacgtcacgc
     6241 cttacccgcc aacgaaccgc gcattgtgct gaacaatggc gtggtttctt ataacgatcg
     6301 ccccattctt aataacctta gctggcaggt gaatccaggt gaacactggc aaattgtcgg
     6361 gccaaatggt gcaggaaaat cgacgttatt aagcctggtt actggcgatc atccgcaagg
     6421 ttacagcaac gatttgacgc ttttcggacg acgtcgcggc agcggcgaaa ccatctggga
     6481 tatcaaaaag catatcggtt acgtcagcag tagtttgcat ctggattacc gggtcagcac
     6541 taccgtgcgt aatgtgattc tttctggcta ttttgattcg attggcattt atcaggccgt
     6601 ttcggatcgc cagcaaaaac tggtgcagca gtggctggat attctcggca ttgataaacg
     6661 tacggctgac gctccgttcc atagtctttc ctggggacag cagcatctgg cgctgatcgt
     6721 ccgcgcactg gtgaaacatc cgacgttgct tattctcgat gaaccactac aggggcttga
     6781 tccgctcaat cgccagctta tccgccgttt tgttgatgtg ctgattagcg aaggtgaaac
     6841 gcaattgttg tttgtttcgc accacgctga agatgcgcct gcctgtatta cccatcgcct
     6901 tgagttcgtg ccggacggtg gactctatcg ctatgtgctg acaaaaatat attgagtcgg
     6961 tagtgctgac cttgccggag gcggcctaag cgccctctcc ggccaacggt tcgacgcatg
     7021 caggcatgaa accgcttctt ttttcagata aaaagcgcaa tcattcataa accctctgtt
     7081 ttataatcac ttaatcgcgc ataaaaaacg gctaaattct tgtgtaaacg attccactaa
     7141 tttattccat gtcacacttt tcgcatcttt gttatgctat ggttatttca taccataagc
     7201 ctaatggagc gaattatgag agttctgctt accggtggta gcggttacat tggaagtcat
     7261 acctgcgtgc aattactgca aaacggtcat gatgtcatca ttcttgataa cctctgtaac
     7321 agtaagcgca gcgtactgcc tgttatcgag cgtttaggcg gcaaacatcc aacgtttgtt
     7381 gaaggcgata ttcgtaacga agcgttgatg accgagatcc tgcacgatca cgctatcgac
     7441 accgtgatcc acttcgccgg gctgaaagcc gttggcgaat cggtacaaaa accgctggaa
     7501 tattacgaca acaatgtcaa cggtactctg cgcctgatta gcgccatgcg cgccgctaac
     7561 gtcaaaaact ttatttttag ctcctccgcc accgtttatg gcgatcagcc caaaattcca
     7621 tacgttgaaa gcttcccgac cggcacaccg caaagccctt acggcaaaag caagctgatg
     7681 gtggaacaga tcctcaccga tctgcaaaaa gcccagccgg actggagcat tgccctgctg
     7741 cgctacttca acccggttgg cgcgcatccg tcgggcgata tgggcgaaga tccgcaaggc
     7801 attccgaata acctgatgcc atacatcgcc caggttgctg taggccgtcg cgactcgctg
     7861 gcgatttttg gtaacgatta tccgaccgaa gatggtactg gcgtacgcga ttacatccac
     7921 gtaatggatc tggcggacgg tcacgtcgtg gcgatggaaa aactggcgaa caagccaggc
     7981 gtacacatct acaacctcgg cgctggcata ggcagcagcg tgctggacgt ggttaatgcc
     8041 ttcagcaaag cctgcggcaa accggttaac tatcattttg caccgcgtcg cgagggcgac
     8101 cttccggcct actgggcgga cgccagcaaa gccgaccgtg aactgaactg gcgcgtaacg
     8161 cgcacactcg atgaaatggc gcaggacacc tggcactggc agtcacgcca tccacaggga
     8221 tatcccgatt aaggaacgac catgacgcaa tttaatcccg ttgatcatcc acatcgccgc
     8281 tacaacccgc tcaccgggca atggattctg gtttcaccgc accgcgctaa gcgcccctgg
     8341 cagggggcgc aggaaacgcc agccaaacag gtgttacctg cgcacgatcc agattgcttc
     8401 ctctgcgcag gtaatgtgcg ggtgacaggc gataaaaacc acgattacac cgggacttac
     8461 gttttcacta atgactttgc ggccttgatg tctgacacgc cagatgcgcc agaaagcaac
     8521 gatcctttga tgcgttgcca gagcgcgcgc ggtaccagcc gggtgatctg cttttcaccg
     8581 gatcacagta aaacgttgcc agaactgagc gttgcggcat tgacggaaat cgtcaaaacc
     8641 tggcaggagc aaaccgcaga gctgggaaaa acgtacccgt gggtgcaggt ctttgaaaac
     8701 aaaggtgcgg cgatgggctg ctctaacccg catccgcacg gacagatttg ggcaaatagc
     8761 ttcctgccta acgaagctga gcgcgaagac cgcctgcaaa aagaatattt tgccgaacag
     8821 aaatcaccaa tgctggtgga ttatgttcag cgcgagctgg cagacggtag ccgtaccgtt
     8881 gtcgaaaccg aacactggtt agccgttgtg ccttactggg ctgcctggcc gttcgaaacg
     8941 ctactgctgc ccaaagccca cgttttgcgg atcaccgatt tgaccgacgc ccagcgcagc
     9001 gatttggcgc tggcgttgaa aaagctgacc agtcgttatg acaacctctt ccagtgctcc
     9061 ttcccctact ctatgggctg gcacggcgca ccatttaatg gcgaagagaa tcaacactgg
     9121 cagctgcacg cgcactttta tccgcctctg ctgcgctccg ccaccgtacg taaatttatg
     9181 gttggttatg aaatgctggc agagacccag cgagacctga ccgcagaaca ggcagcagag
     9241 cgtttgcgcg cagtcagcga tatccatttt cgcgaatccg gagtgtaaga aatgagtctg
     9301 aaagaaaaaa cacaatctct gtttgccaac gcatttggct accctgccac tcacaccatt
     9361 caggcgcctg gccgcgtgaa tttgattggt gaacacacgg actacaacga cggtttcgtt
     9421 ctgccctgcg cgattgatta tcaaaccgtg atcagctgtg caccacgcga tgaccgtaaa
     9481 gttcgcgtga tggcagccga ttatgaaaat cagctcgacg agttttccct caatgcgccc
     9541 attgtcgcgc atgaaaacta tcaatgggcg aactacgttc gtggcgtggt gaaacatctg
     9601 caactgcgta acaacagctt cggcggtgtg gacatggtga tcagcggcaa tgtgccgcag
     9661 ggtgccgggt taagttcttc cgcttcactg gaagtcgcgg tcggaaccgt attgcagcag
     9721 ctttatcatc tgccgctgga cggcgcacaa atcgcgctta acggtcagga agcagaaaac
     9781 cagtttgttg gctgtaactg cgggatcatg gatcagctaa tttccgcact cggcaagaaa
     9841 gatcatgcct tgctgattga ctgtcgttca ctggggacca aagcagtttc catgccgaaa
     9901 ggtgtggctg tcgtcatcat caacagtaac ttcaaacgta ccctggttgg cagcgaatac
     9961 aacacccgtc gtgaacagtg cgaaaccggt gcgcgtttct tccagcagcc agccctgcgc
    10021 gatgtcacca ttgaagagtt caacgctgtt gcacatgagc tggacccaat cgtggcgaaa
    10081 cgcgtgcggc atatcctgac tgaaaacgcc cgcaccgttg aagctgccag cgcgctggag
    10141 cagggcgacc tgaaacgtat gggcgagttg atggcggagt ctcatgcctc tatgcgcgat
    10201 gatttcgaaa tcaccgtgcc gcaaattgac actctggcag aaatcgtcaa agctgtgatt
    10261 ggcgacaaag gtggcgtacg catgaccggc ggcggatttg gcggctgtat cgtcgcgttg
    10321 atcccggaag agctggtgcc tgccgtacag caagctgtcg ctgaacaata tgaagcaaaa
    10381 acaggtatta aagagacttt ttacgtttgt aaaccatcac aaggagcagg acagtgctga
    10441 acgaaactcc cgcactggca cccgatggtc agccgtaccg actgttaact ttgcgtaaca
    10501 acgcagggat ggtagtcacg ctgatggact ggggtgcgac tttactttca gcccgtattc
    10561 cgctttccga tggcagcgtc cgcgaggcgc tgctcggctg tgccagcccg gaatgctatc
    10621 aggatcaggc cgcgtttctg ggggcctcta ttggtcgtta tgccaaccgc atcgccaata
    10681 gccgttatac ctttgacggt gaaaccgtga ccctttcgcc aagtcagggc gttaaccagc
    10741 tgcacggcgg cccggaaggg ttcgacaaac gtcgctggca gattgtgaac cagaacgatc
    10801 gtcaggtgct gtttgccctg agttcagatg atggcgatca gggcttcccg ggtaatctcg
    10861 gcgcaacggt gcaatatcgt ctgaccgacg ataaccgtat ctccattact tatcgcgcca
    10921 cagttgataa accttgcccg gtgaatatga ctaatcacgt ctatttcaat cttgacggcg
    10981 agcagtctga cgtgcgcaat cacaagttgc agattctggc ggacgaatat ctgccggttg
    11041 atgaaggcgg cattccgcac gacggcctga aatctgtcgc cggaacgtct tttgatttcc
    11101 gcagcgccaa aatcatcgcc agtgagtttc ttgccgacga cgatcagcgc aaagtgaaag
    11161 gttacgatca cgcattcttg ttacaggcca aaggcgatgg caagaaagtg gcggcgcatg
    11221 tctggtcagc agatgaaaaa ttgcagctga aggtctacac caccgctccg gctctgcaat
    11281 tctactccgg caacttcctc ggcggcacac cgtcgcgggg aacagaacct tacgccgact
    11341 ggcaagggct ggcgttggaa agcgagtttc tgccggacag cccgaaccac cctgaatggc
    11401 cgcaaccgga ctgcttcctg cgtcctggcg aagagtattc cagcctgacg gaatatcagt
    11461 ttattgctga gtaatgttaa gccctcgtca tgagggcttt atctcatatt gctcaaatca
    11521 ccagcaaaca ccgacatatt tgcaactcaa tattcacaac aaccttacac tgcgccacta
    11581 ttttcgctat ggttatgcgt aagcattgct gttgcttcgt cgcggcaata taatgagaat
    11641 tattatcatt aaaagatgat ttgaggagta agtatatggc tgtaactaag ctggttctgg
    11701 ttcgtcatgg cgaaagtcag tggaacaaag aaaaccgttt caccggttgg tacgacgtgg
    11761 atctgtctga gaaaggcgta agcgaagcaa aagcagcagg taagctgctg aaagaggaag
    11821 gttacagctt tgactttgct tacacttctg tgctgaaacg cgctatccat accctgtgga
    11881 atgtgctgga cgaactggat caggcatggc tgcccgttga gaaatcctgg aaactgaacg
    11941 aacgtcacta cggtgcgttg cagggtctga acaaagcgga aactgctgaa aagtatggcg
    12001 acgagcaggt gaaacagtgg cgtcgtggtt ttgcagtgac tccgccggaa ctgactaaag
    12061 atgatgagcg ttatccgggt cacgatccgc gttacgcgaa actgagcgag aaagaactgc
    12121 cgctgacgga aagcctggcg ctgaccattg accgcgtgat cccttactgg aatgaaacta
    12181 ttctaccgcg tatgaagagc ggtgagcgcg taatcatcgc cgcacacggt aactccctgc
    12241 gtgcgctggt gaaatatctt gataacatga gcgaagaaga gattcttgag ctgaatatcc
    12301 cgactggcgt gccgctggtg tatgagttcg acgagaattt caaaccgctg aaacgctatt
    12361 atctgggtaa tgctgacgag atcgcagcga aagcagcggc ggttgcaaac cagggtaaag
    12421 cgaagtaaac gtcattcgtt taaaatgaga aagccgactg caagtgagtc ggcttttttg
    12481 ttgctaacaa tggagcacat tgcctgatgc gacgcttcgc gacttatcag gcctgtggtg
    12541 attcatcgga tacgccactc tgacggcgca tccgacaatt aaaccttacc cgcgacgcgc
    12601 ttttactgca ttcgccagtt gacgtaacag agcatcggta tcttcccagc cgatgcaggc
    12661 atcggtgatg ctcttaccgt aggccagcgg ctccccgctc tcgaggctct gattgccttc
    12721 caccagatgg ctttccacca tcacgccaat aatggccttt tcgccaccgg caatctgctg
    12781 gcaaacgtca gcacaaacat ccatctgctt tttgaattgt ttggacgagt tagcatggct
    12841 gaaatcgatc atcacctgtg ctggcaggcc tgctttgttc agcccttctt tcacttcagc
    12901 aacgtgcttc gcgctgtagt taggctcttt accgccgcgc agaatgatat ggcaatcgcc
    12961 gttaccgctg gtattcacaa tcgccgaatg cccccatttc gttacggaca ggaagcagtg
    13021 cggcgcaccg gcggcattaa tggcatcgat agccacttta atcgtaccgt cggtgccatt
    13081 tttgaagccg actggacaag aaagccctga tgccagttcg cggtgcacct gcgattcggt
    13141 ggtacgtgcg ccaattgcgc cccagctcat caggtcagcg agatattgtg gggtgatcat
    13201 atcgagaaac tcacccgccg ctggcagacc gctgtcgtta atatcaagca gcaatttacg
    13261 ggctatacga agaccgtcgt tgatctggaa gctgttatcc atatgcggat cgttaatcag
    13321 ccctttccag cccaccgtgg tacgcggctt ttcaaaatag acgcgcatta cgatttccag
    13381 ctcatctttc agctcttcac gcagcgccag caagcgagtg gcatactctt tagccgcgac
    13441 aggatcatga attgagcacg ggccaatcac caccaacagg cgatcatcat tacctttcag
    13501 gatcttatgg atcgcttttc gggcatgggc aaccgtattc gcggcatttt cagtagcggg
    13561 gaatttttcc agcaatgcga caggaggaag taactctttg atttctttga tgcgtaaatc
    13621 gtcgttctga taattcatgt ctgttccagt gttgccatac ttatcttagt gaatgcaata
    13681 cttccaatct atatcttccg tcagaatgtg taaacggggt tttacattat gaacggatta
    13741 atcctggaat tagcaagaaa aacgccagat tgtcgcgaaa aacgagatct ctcctacaat
    13801 ttctaactgt aactcctttg tttgttaatt atttcaagat tctctgctgc gtttcataaa
    13861 ctggctgaaa tcttaaacca atgccttata ttcacctgca aatgcactgt tggaagaggt
    13921 tatccgacat aacgaccata acaggagcat cctatgaaaa tgacaaaact ggccacatta
    13981 tttctgactg ccactctaag ccttgccagc ggtgccgcac tggctgccga tagcggagcg
    14041 caaactaata acggccaggc aaacgccgca actgatgcgg gccaggtagc ccctgacgcc
    14101 cgtgaaaatg tcgcgccaaa taacgtcgac aataacgggg taaataccgg ttctggcggc
    14161 acaatgctgc attcggatgg ttcttcaatg aacaatgacg gaatgaccaa agatgaagag
    14221 cacaagaaca ccatgtgcaa agatggtcga tgtccggata tcaataaaaa ggtacaaacc
    14281 ggtgatggca tcaataatga tgtcgatacc aaaaccgacg gtaccacgca gtaaccctgc
    14341 aaaatagcca ataatgggag agcggagtct ctccctttta atttttcact tctaaaagca
    14401 gtatgatgtc ataaagttaa gtgagaataa ttaagggatg actcgtcatg gcgcactcac
    14461 actcacacac gtcctcacac ctgccagaag ataataatgc tcgtcgcttg ttgtatgctt
    14521 tcggcgtgac tgccgggttt atgctggtag aagtcgttgg tggttttctt tctggttctc
    14581 tggcattgct ggccgatgcg ggtcatatgt tgaccgatac tgccgccctg cttttcgccc
    14641 tactcgccgt gcaattttca cgtcgtcctc ccaccattcg ccacactttc ggttggctaa
    14701 gactgaccac gctcgcggcg tttgtgaatg ccatcgccct cgtggtaatt accattttga
    14761 ttgtctggga ggcgatagaa cgcttccgca cgccgcgtcc ggtcgagggc ggcatgatga
    14821 tggcaattgc cgtggctggg ttgctggcaa atatactttc tttctggtta cttcatcacg
    14881 gcagcgaaga gaaaaacctc aacgtacgag cagcggcatt gcatgtgctc ggggacctgt
    14941 tgggttcggt tggggctatt attgccgcgt tgattattat ctggaccggc tggacacctg
    15001 ctgaccccat tctctcgata gtggtgtcgc ttctggttct gcgtagcgcg tggcgactgt
    15061 tgaaagatag cgtgaatgaa ttacttgaag gtgcaccggt atcgctggat atcgctgaac
    15121 tgaagcgtcg tatgtgccgg gaaatcccgg aagtccgcaa tgtgcaccat gtacatgtat
    15181 ggatggtagg tgagaagccg gtgatgacgc tgcatgtgca ggtgatcccg ccacacgctc
    15241 acgatgcctt gttggatcag atccaacact acctgatgga tcactatcag attgagcacg
    15301 ccaccattca gatggaatat caaccttgtc atgggccaga ctgccacctg aatgaagggg
    15361 tgtctggcca ttcacatcat caccattaat gggacagcgc gcatgagcct ctttcacgtg
    15421 cgctgttgat ccacatccga ctgccgttga gcgcaataaa ggtcaggatg atgtactcca
    15481 gagacatggc gtaaacgccc tgaagtgcaa aaataacgac gctaatcacg ttaataatca
    15541 cccacaacag ccagttttcc acatacttac gcgtcatcag aagcattgcc acgatagata
    15601 acaccatcat gcatgaatcc cagaacggga aagcgtccgg ttgcagttca ggcatcacaa
    15661 cctgtaatcc taatgcttgc atgatcatga ctgccacgcg ggtcaaaaat gcaaacaccg
    15721 gattgataaa taccgtcatc agaccaatcg aaacaacgca aaccgccaac cagctgagcg
    15781 ccttcggcaa tggtaaccag cgaattttca actccgcctc gttctgactg gtttgtcgcg
    15841 accacgcata ccaaccgtaa atattcgcgg caaagaaaaa cacctgtaat agcaggctgg
    15901 catacagctg aatctgaaag aaaataatgc caaacaaggt gacgttgatc aggccaaaga
    15961 agtagttgct gatcttctcc agactggcaa ggccaataca cagcaacccg gcgatcgtgc
    16021 ctaccgcttc gatccatgag agatcataac cgcctgctcc tattggtata tgtaccagga
    16081 tattctgcac actaaaaaaa tccatctttc ccccaaagcg taacgttacg ccttattatt
    16141 cgttatccac gtagtgtagc cgcaaaatcc agcatacgat tgagcggcac cagcgccctc
    16201 tctcgcagcc tttcatcaac atgaacctcg tgattgcttc cttcctgttc taatgcctct
    16261 gcgatggcct gaaggccatt catggccatc cacggacaat gcgcgcagct gcggcaggtt
    16321 gcaccctcac ctgcggttgg tgcttccagt aactctttat ctggcaccgc ctgctgcatt
    16381 ttgtagaaaa taccccgatc ggttgccaca ataagcctct gatgtggcaa tgttttcgca
    16441 gcagcgatca gttgactggt ggaaccgacc gcatccgcca tatcgacaat agcttgtggt
    16501 gattctggat gcaccagtat ggcagcatcc gggtattctt cttgcaagcg ggttaacgcc
    16561 tgagtcttaa attcatcatg cacaatacag gcaccctgcc agcatagaat gtctccaccc
    16621 gtctgttttt gcacgtaacg ccccagatgt ttgtcgggtg cccagatgat tttttcaccc
    16681 aaactatcaa gatgatcaat aagttcgacg gcaatgcttg aagttaccac ccaatctgcg
    16741 cgcgctttta ccgcagcaga agtgttggcg tagacgacga cagtacgatc gggatgggca
    16801 tcgcaaaatg cgttaaattc ttcaacaggg cagccgagat ccagtgaaca ttcagcctga
    16861 agtgtcggca tcagaattgt tttttccgga ctgagaattt tggcggtttc tcccataaat
    16921 ctcaccccag cgactaacaa agtagaagcg ggatgctttg caccgaagcg cgccatttcc
    16981 agagaatcag aaatacagcc accggtttct tctgccagtt gttgaatttc gggatcggta
    17041 tagtagtggg caaccatcac cgcattacgt tcttttagca gacgttttat cttctcgcgg
    17101 taatacgctt tttcatcaat gcttaacggc gtcggcttcg gggggaaagg ataaatcgcc
    17161 gtgtctggat caaacattac gctcatctta ccatctcgtt ttacaggctt aacgttaaaa
    17221 ccgacattag cgtaatattc gctgttatcg gtgtaatttg ttttatatgc taaacaagat
    17281 agtcgaatgg tggggaaaag tcactcgaat tacgcctaat tccaccgacg tagagttgct
    17341 tgcacagtaa gctctgacga tgtcatcagg tttacaaagt gaaattgagg atgcctgaat
    17401 agcaaaaaag cgcctttagg gcgctttttt acattggtgg gtcgtgcagg attcgaacct
    17461 gcgaccaatt gattaaaagt caactgctct accaactgag ctaacgaccc atacgggtgt
    17521 tacctgaaag atttctactc ggggccaatc tttaagattg gtgtgccgtg caggatgact
    17581 cgcttcgctt gccctacggg ccgttgctgg cgcaacgtta tccttcacgc ttaacatctg
    17641 agtttgatgt taaaattggt gggtcgtgca ggattcgaac ctgcgaccaa ttgattaaaa
    17701 gtcaactgct ctaccaactg agctaacgac ccgagtggtg ggtgatgacg ggatcgaacc
    17761 gccgaccccc tccttgtaag ggaggtgctc tcccagctga gctaatcacc cgataatgcg
    17821 ctggatacta caaactactt ccaccgaacc catcgtaatt agtggtgggt cgtgcaggat
    17881 tcgaacctgc gaccaattga ttaaaagtca actgctctac cgactgagct aacgacccgg
    17941 tggtgggtga tgacgggatc gaaccgccga ccccctcctt gtaagggagg tgctctccca
    18001 gctgagccaa tcacccaata ctacgctgga tactacaact aactatgtta gtggtgggtc
    18061 gtgcaggatt cgaacctgcg accaattgat taaaagtcaa ctgctctacc aactgagcta
    18121 acgacccact ttttcgttgc tttcggtttg tttgatatcc cgtggcaacg gcggcatata
    18181 ttactgattt cagacttgag cgcaacaaaa atttcgatgc agatcactca actgcttatg
    18241 attcgcacga cacgaccaga aataatgcaa cttctggtcg tgtgttatgc attacatcgc
    18301 gttcagacgt ttttgtgcct gtttagcgcc atcagtacca gggtatttac tgataacctg
    18361 ctggtatacg gctttcgctt ttgcggtgtc acctttgtcc tgcatgatga cgccgacttt
    18421 aaacatcgca tcagcagcct ttggtgactt cggatagttt ttcactaccg aagcaaaata
    18481 gtacgccgca tcatcttttt tacccttgtt gtagtttaac tgacccagcc aataattggc
    18541 gtttggcagg taagttgaat cagggtaatt tttgatgaaa ttctgaaatg ccaccattgc
    18601 gtcatcctgg cgggatttat cctgcaccag cgcaatagct gcattgtaat ccgtgtttgc
    18661 atcgccgctt ttcaccggcg cgccagcatt cgcagtacca gcatcagctg tcggcgtcgt
    18721 tgatgccgcc gcaccgcttt gatcgctgct ggttgattgc gccgctgcac caccgctgct
    18781 gaggctgtcg atctgcaaca ggatctgctt ttgccgctcc acgacctgat tcagttgata
    18841 ctgattttcc tgaatctgac cacgcaggga atcaatatcg gattgattat cagaaagttg
    18901 ttgctggagt tgggttaaaa gctggctgtg agcattagaa atacgctcaa gttgagtgac
    18961 gcggtcttcg accgagcctg agccgacact actgattggt gcctgagcaa aagcggccca
    19021 gggggccgct ataccaacca gtaacgacag actcaatagt tgatgtctga agttactgct
    19081 catgcaattc tcttagtaaa ccagtaccgc acgacggttt ttggagtatg ccgcttcgtc
    19141 atgacccagt actgcaggtt tttctttacc gtaagaaacg atggagatct ggtctgcaga
    19201 aacgccttta ccctgcaggt acatcttaac ggcgttcgca cgacgttcac ccagggagat
    19261 gttgtattcc ggagtaccac gttcgtccgc gtgaccttct acggtgactt tgtaagacgg
    19321 gttgctacgc aggaagtttg catgtgcatc cagcatttga gcgaagtcag aacggatatc
    19381 gtacttgtcc agatcgaagt aaacgatgtt gttctgctgc agctgttgca tttgcagacg
    19441 agcctgctct tcggaagaca tgttgccgtt gccgccgttc gcatccatac cagtgccggc
    19501 acccagcatg ccttcgctgc cgtcattgct ggcgttcttg ttggaagaac atgccgcaat
    19561 tgccataaca ggcagagcaa tcatcagccc tttcagcact ttgttcagtt gcatttcaat
    19621 gattccttta ctattcaatt aattattatc acagatacgg cgaccaggca gggaatttga
    19681 cctgtccatc agttgccgga agacgcgctt tgaaacgccc atctgtagaa accaaattca
    19741 gcacggatcc catcccctga gaagagctgt agattaccat agtgccatta ggtgccagac
    19801 ttggcgtttc atccaggaac gtggacgaca gaacttgtac gcctcccgtt gccagatctt
    19861 gtttggcaat gtgctgctgc ccaccattgg agctgaccat taccataaat ttaccgtcgc
    19921 tgctgacatc cgcatcctgg ttctgcgaac cttcccaggt aatacgttgt ggcgcaccgc
    19981 cgttgatatt cactttataa acctgcggac gaccggcctg gtcagaagta aatgccaggt
    20041 tctggctatc cgggaaccag gtcggttcgg tattgttact gcgaccatca gtcacctggc
    20101 ggatctgacc agaagccaaa tccattacgt acaggttcag actaccggtt ttcgacaagg
    20161 cgaatgccag tttgctgccg tctggcgaga atgcaggtgc accgttgtga cgcgggaatg
    20221 aagccacctg acgtacagcg ccatttgcca gcgtctgaat aaccagcgcg gaacgaccgc
    20281 tttcgaaggt cacataagcc agtttagaac cgtctggtga ccacgccgga gacatcagcg
    20341 gctgcggtga acggtgaacg acaaactggt tgtaaccgtc atagtcagat acgcgcagtt
    20401 catacgggaa ctgaccgccg ttggtctgaa caacgtaggc aatacgggta cggaacgcac
    20461 ctttaatgcc ggtcagtttt tcaaacactt catcactggc ggtatgacca gcataacgca
    20521 gccactgctt gttcactttg tacgagttct gagcaagtac agtacccggt gcgccgccag
    20581 tgtcaacaag ttgataagca acattgtaag aaccatccgg attcggagtg acctgaccga
    20641 caactacagc gtcaatgccc agtgcggacc atgcagatgg ttgtacttcc tgcgcactac
    20701 ccggctgctg tggcagacga gcgcgatcta acggattaaa tttaccgctg ttacgcaagt
    20761 cagcagcaac gatgccgcca atatcttcag gtgccgcacc aggccccgcc cactggaaag
    20821 gaacaacacc aataggacga ccggaatcta caccgctgtc gatcacaatg cggacttcag
    20881 catgcagaac tgatgcccac agtatgagaa aaccaaatgc tactcgtaat gcctgcttca
    20941 tcatatctcc cttatctgga ccgatggccc acgataattt agcagaatgt taacaaacta
    21001 aaatacacaa aactaccaga accccgtggc aaccggtgcc tgatgttgac cgtccgaaca
    21061 gtcaacatcg cgattacggt ttgaagtcca atggcgcgtt tttgaacact tcatatactg
    21121 cctggcttgg tggtttcggg atcttcgcaa gtttagctgc tgccaacgca gcctgacaaa
    21181 gtgcgggatc gccaccttca ggtttgatat ccagtaacat accatcgggt gccagtttta
    21241 tgcgcagcgt acaggttttg cctgcatagg acgatgcgtc atagaactta ctttcgatag
    21301 cagatttaat ctgcccggca tagttattga tatcggcccc tgatgcgcca ttgtttttag
    21361 aattaccact cccggcaggc gaagcattgt tccctttcgc ccctcccccc gttttcggtg
    21421 cattcttacc agagcttagc tcaccgaaaa tatcatctgc ctctgcggcg gctttttctg
    21481 ccgctgcttt cttatcagct gctgcctttt cagcagccgc tttttctgct gcttttttgt
    21541 cggctgcagc tttcttatca gctgcagcct tttcagcagc cgctttcttc tcagcttctg
    21601 ctttggcttt ttcagcagct tcagttgccg ctttctttct tgcttcagct gcagctgctt
    21661 ctgccgcttc cgctttcttc ttcagtgccg cagcggctgc ctcggctttt ttctgcgctg
    21721 cggctttggc ggcttctgct tctgcttttt tctttgcgtc tgcagccgct ttcttcgctg
    21781 cttcttccgc agctttagca tctgcttcgg ccttcgcttt agcatctgcc gccgctttcg
    21841 ctgccgcctc ttcagcttgc ttctgcttta actcggcctg ttttgcggct tcttcagcct
    21901 gctttttctg ctcctgagcc gctaaccgct ctttctcaag ttgcttcagg cgttcctgtt
    21961 cagccgcttg tttctcacgc agttcttcag cagcctgctg ttccttcatc ttgcgctgtt
    22021 catcagaacg cttcgcgctt gattcctggc tttgcatgcg tttgtactgc tcaactaccg
    22081 cacctgaatc aaccatgaca gcgtcgatgg acgaaccacc gccgcctccg gctgaagctt
    22141 ctatattctc atcgaacgaa ctccagatca gcgccgcaaa taagatgaca tgcagcactg
    22201 ctgaaataat tatcgcccgc ttgagcttgt cgttttgttc ggttgccttt gacactctcg
    22261 gtttccaaaa actgttcgcc tgttacccgc tctctttcaa gcaagggaaa cgcagatgtt
    22321 tagataggct gcgtcattaa accaaccgat ttcacacccg cactatgtaa caagttcagt
    22381 gctttaatta tttcatcgta aggcacatct tttgcgccac cgatcagaaa gaccgttttc
    22441 gggttggcct tgaagcggct ggacacttcc gccaccacct gctctggtgg taaacgctcc
    22501 aggcgatctt tctcaaccac cacggtgtac tgaccaatac cagacacttc aacaatcact
    22561 ggcggattat cgttactgct caccgcctgt gattcagtag cgtctggcag atcgacctcc
    22621 acgctctggg tgatgatggg cgctgtcgcc atgaagatca gcaacagcac cagcagtacg
    22681 tccaacaacg gtacaatgtt gatttcggac ttaagttcgc gacgacctcg tccacgcgct
    22741 ctggccatga cttacccctt attgctctcg ctaacggtaa acgcctggcg gtgcagaatc
    22801 gcggtaaact cttccataaa gttgtcgtaa ttcagttcca gtttgtttac gcgctggttg
    22861 aggcggttgt aggccataac tgccgggata gcggcaaaca gaccaattgc agtcgcaatc
    22921 aacgcttctg cgataccggg cgcaaccatt tgcagtgttg cttgttttac cgccccgagg
    22981 gcgataaagg catgcatgat cccccagacc gtaccaaaca gaccaatata cgggctgatg
    23041 gagccaaccg taccgaggaa cggaatgtgc gtttccagat tttcaagttc acggttcatg
    23101 gagatacgca tagcacgcga cgccccttcc actacggctt ccggcgcatg gctattggca
    23161 cgatgcaggc gcacaaactc tttgaacccg ctgtaaaata tttgttccga accagtcaga
    23221 ttatcccgtt tcccctgact ctcttgatag aggcgagaga gttcgattcc agaccagaat
    23281 ttatcttcaa acgcttcggc ttcgcgcgcc gctgcgttaa gaatacgggt ccgctggata
    23341 ataatggccc aggatgcgat tgaaaaacca atcaaaatca acatgataag tttaaccaga
    23401 aggctagcct tcaggaacaa atcaaggata ttcatgtcag tcactgctta aactccgcga
    23461 caatagactt gggaagcgca cgaggcttca ttttgagtgg gtcaacgcaa acaaccagaa
    23521 cctctgcttc attcagcaaa gtattctcgg cgttgacaat acgttgcgtg aaaaccaaag
    23581 aggtgccacg cattgatgtt atttcagtct gtatttcgag catatcgtcg agccgcgcag
    23641 gtgcgtaata ttccaccgtc attttacgta ccacaaaggc aacgcgttca gccatcagcg
    23701 cctgctgact gaagtgatga tgacgcagca tctctgtgcg tgctctttca taaaaagcga
    23761 cgtaactggc gtggtacacc acaccaccgg catcggtatc ttcatagtag acgcgagccg
    23821 gccatcgaaa cagcgttgta ttcactttac atcccggtaa tgcaacaaaa gttagagctt
    23881 ttaaacgctg ctactatacg cgcgcgattg aggtttggga atgagggcaa gttaagggag
    23941 cgtaaaaatt tatgggcctg gagcggccca taatttttta aagagaattc tgatttaaaa
    24001 gaagaagaaa atcagcccga taatgagaac aatatcggca agcaatgggc aaaaaatccc
    24061 ttgccaaaga accttctgcg gacgaaagcc cacgccgtga atcacaccag cacagacggc
    24121 ccacatcagc aataaaccat gccagatttc cagttcactg gtcttcgcgg caaaacgaga
    24181 tgggtcccaa aacatacatc ctgctaacag aagcgccatc acgaaggaaa gcgcccgcag
    24241 ggggcgcttg tccattaccg catacaaggt cgcgataatc ttactcatca gatgtcttct
    24301 tgaccggctt tgcctgattc gacgtgttca agcgccagcg cggtgattac cccaaacgaa
    24361 caggcaagaa gcgttcccag aatccatgcg aaataccaca tttttagctc cttacttagt
    24421 acagagagtg ggtgttacgt tcaatatctt ctttggtgat acgaccgaac attttccagt
    24481 aacaccaggc ggtgtagagc agaatgatcg gtaccagaac caccgcaacc caggtcatga
    24541 cgttaagcgt cagctggctg gaagttgcat cccacattgt cagacttgcg ttcatcatgg
    24601 tgctggacgg catcacaaac gggaacattg cgataccggc tgtcaggatg atgcaggcca
    24661 gcgtcaggga ggagaacaca aacgcccacg cggctttatc catacgtgca gtcaggatgg
    24721 tcagcagcgg cagaaccaca cccagtgccg gaatagccca cagaattggc gtgttgttga
    24781 agttaaccag ccatgcgcca gcttcacgaa ccacttcttt attcagtggg ttagaggctg
    24841 cgtaatggtc cattgtcgat ttcacgacat aaccatcgat accgtacatc acccatacgc
    24901 cagccagtgc gaaacagacc agtgtcacca gcgcagccac ctgagccgtt gcacgggtac
    24961 gcaggtgcag ttcgcccacg gtacgcattt gcagataggt tgcgccctga gtaatgatca
    25021 tccctacgct caccacgcct gccagcaggc cgaacgggtt aagcaactgg aagaagttac
    25081 cggtgtagta cagacgcaga tattcatcaa cgttgaacgg tacgccctgc aacaggttac
    25141 cgaacgcaac accaattacc agcggcggaa cgaagctacc aatgaagatg ccccagtccc
    25201 acatgttacg ccagcgggtt tcttcaatct tggagcggta gtcaaaaccg accggacgga
    25261 agaacaaaga cgccagcacg aggatcatcg ccacatagaa gccggagaac gcagcggcat
    25321 agaccatcgg ccaggcagca aagagtgcgc cgcccgcggt gatcagccaa acctggttac
    25381 cgtcccagtg tggtgcaatg gagttaatca taattcgacg ctcggtgtcg ttacgaccga
    25441 ggaaacgggt gagcatgccc acccccatgt cgaaaccgtc agtgactgca aaaccaatca
    25501 gcagaacgcc aaccagcagc caccagataa aacgcaatac ttcataatcg atcatttgac
    25561 gactcctgtc ttagcgtgcc ggctgagtag tcgtggaaga ctgctcaaag tgatagcgac
    25621 cggttttcag gctgcttggg ccgaggcgtg caaatttgaa cattaagaac aattctgcca
    25681 ccaggaacag ggtatacagg ccgcaaatca gcaccattga gaagatgaga tcgcctgcgg
    25741 tcagtgacga attcgccaca gctgtcggca gcacttcacc gatagcccac ggttggcggc
    25801 catattcagc cacgaaccag cccgcttcta cagcaatcca cggcagcgga ataccgtaca
    25861 gcgcggcgcg cagcagccat tttttctcgc caatgcggtt gcggatgaca ctccagaaag
    25921 agagcgcgat gattgccagc agcaggaagc cacacgccac cataatacgg aacgcgaagt
    25981 acagcggcgc tacacgcgga atggagtctt tagttgcctg ttgaatctgc gcttcagtcg
    26041 catcagccac gtttggcgta tagcgtttca gcagcagacc gtaaccgagg tctttcttca
    26101 tgctattgaa ctggtcacga accgcctggt cggtagaacc agaacgcagt tgttcgagca
    26161 gagagtacgc cttcatcccg ttacgaatgc gttcttcatg ctgcaccatc agctctttca
    26221 ggccgataac cggggtatcc acggaacgcg ttgcaatgat gcccagtgcg taagggatct
    26281 gaatcgcaaa tttgttcgtc tcctcttcct gatcaggaat gccgaacaga gtaaaggcag
    26341 caggcgcagg ttgcgtttcc cactcggctt caatagcagc cagtttggtt ttctgcacgt
    26401 cgcccatttc gtagccggat tcatcaccca gaacaataac agacagaaca gcagccatac
    26461 cgaagctggc agcgatagca aaggagcgtt tagcgaaggc gaagtcacga cctttcagca
    26521 tataccatgc gctgataccg aggatgaaca tcgcgccagt cacataacca gacgctacag
    26581 tgtgaacgaa tttcacctga gcaaccgggt taagcaccag ctcggagaag ctcaccatct
    26641 ccatacgcat agtttcaaag ttgaaatcgg acgcgattgg gttttgcatc cagccgttcg
    26701 caaccagaat ccacagtgcg gacaggtttg aaccgagcgc caccagccag gtgacacaca
    26761 tatgctgaac tttacccaga cgatcccaac cgaagaagaa cagacctaca aaggtggatt
    26821 cgaggaagaa ggccatcaga ccttcgattg ccagcggcgc accgaagata tcccctacat
    26881 agtgggaata gtaagaccag ttagtcccga actggaactc catggtcaga ccggtagcca
    26941 cacccagagc gaagttgata ccaaacaact tgccccagaa cttggtcata tctttataaa
    27001 tctgtttgcc ggagaggacg tagaccgttt ccataatggc cagcaggaac gccataccga
    27061 gcgtcagtgg cacaaaaagg aagtggtaca tcgcggtcaa ggcaaactgt aagcgcgaca
    27121 gttcgactat atctaacatc atgactcctt gctcatcgca tgaagactcc gagagtgaac
    27181 cccgttagaa agggtcacac gcatgcccca atagaaattt gtttgaacac tttcatcata
    27241 ccatgtgaat gcacacaaaa acggcgtaaa gaaaggttgc aaacatgtta ataaaaactc
    27301 aaattgatcc cacgtatata ttacgccgca aaatccttac aataaacagg tttttgttga
    27361 agcaaatttg catttttcga cggtgatcaa tttatagcta aattaccgcc tttcagccaa
    27421 tttgatcgag aacaatttat ctctttttga tgcccatttc caagacttat acattgataa
    27481 atatcaattc ctacaaatta tgttaatcat gtttcaataa ggtaacttta gtgacattta
    27541 tgtttaaaat gtgtgagtta taaggctgaa attagtttcg gttggcaatt aaagataaaa
    27601 agagacgaag caaaaacagc acggttgacc ttaattcttt ttttggtcac tggctaatac
    27661 gacg
//
LOCUS       DAZPEB010000030        27513 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZPEB010000030 DAZPEB010000000
VERSION     DAZPEB010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27513
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.030"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..190
                     /locus_tag="ACHURJ_001186"
     CDS             <1..190
                     /locus_tag="ACHURJ_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFR1280210.1"
                     /translation="TGTVLGLNARDSKHAGRMFSMHNCQINPGAGPEITTPWKSYQHE
                     KNRNGLMPLIRRRTMLQL"
     gene            380..493
                     /gene="yhiY"
                     /locus_tag="ACHURJ_001187"
     CDS             380..493
                     /gene="yhiY"
                     /locus_tag="ACHURJ_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFR1280211.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            556..1623
                     /locus_tag="ACHURJ_001188"
     CDS             556..1623
                     /locus_tag="ACHURJ_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR1280212.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHMRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            1620..4355
                     /gene="rbbA"
                     /locus_tag="ACHURJ_001189"
     CDS             1620..4355
                     /gene="rbbA"
                     /locus_tag="ACHURJ_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFR1280213.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMSAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSTLSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDMWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            4355..5479
                     /locus_tag="ACHURJ_001190"
     CDS             4355..5479
                     /locus_tag="ACHURJ_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1280214.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            5552..5827
                     /locus_tag="ACHURJ_001191"
     CDS             5552..5827
                     /locus_tag="ACHURJ_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFR1280215.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            5824..6183
                     /locus_tag="ACHURJ_001192"
     CDS             5824..6183
                     /locus_tag="ACHURJ_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFR1280216.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLLEYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(6303..6704)
                     /gene="nikR"
                     /locus_tag="ACHURJ_001193"
     CDS             complement(6303..6704)
                     /gene="nikR"
                     /locus_tag="ACHURJ_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFR1280217.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(6710..7516)
                     /gene="nikE"
                     /locus_tag="ACHURJ_001194"
     CDS             complement(6710..7516)
                     /gene="nikE"
                     /locus_tag="ACHURJ_001194"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFR1280218.1"
                     /translation="MTLLNVSGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDETVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRATEKV"
     gene            complement(7513..8277)
                     /gene="nikD"
                     /locus_tag="ACHURJ_001195"
     CDS             complement(7513..8277)
                     /gene="nikD"
                     /locus_tag="ACHURJ_001195"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFR1280219.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKAVYPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(8277..9110)
                     /gene="nikC"
                     /locus_tag="ACHURJ_001196"
     CDS             complement(8277..9110)
                     /gene="nikC"
                     /locus_tag="ACHURJ_001196"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFR1280220.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSH
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVSGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(9107..10051)
                     /gene="nikB"
                     /locus_tag="ACHURJ_001197"
     CDS             complement(9107..10051)
                     /gene="nikB"
                     /locus_tag="ACHURJ_001197"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFR1280221.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(10051..11625)
                     /gene="nikA"
                     /locus_tag="ACHURJ_001198"
     CDS             complement(10051..11625)
                     /gene="nikA"
                     /locus_tag="ACHURJ_001198"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR1280222.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKLSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKTPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(11736..12323)
                     /gene="acpT"
                     /locus_tag="ACHURJ_001199"
     CDS             complement(11736..12323)
                     /gene="acpT"
                     /locus_tag="ACHURJ_001199"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFR1280223.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(12378..13414)
                     /locus_tag="ACHURJ_001200"
                     /pseudo
     CDS             complement(12378..13414)
                     /locus_tag="ACHURJ_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
     gene            13546..14763
                     /locus_tag="ACHURJ_001201"
     CDS             13546..14763
                     /locus_tag="ACHURJ_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1280224.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKSWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(14767..15324)
                     /gene="dcrB"
                     /locus_tag="ACHURJ_001202"
     CDS             complement(14767..15324)
                     /gene="dcrB"
                     /locus_tag="ACHURJ_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFR1280225.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAENNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(15397..16062)
                     /locus_tag="ACHURJ_001203"
     CDS             complement(15397..16062)
                     /locus_tag="ACHURJ_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFR1280226.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSILGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            16283..16528
                     /gene="tusA"
                     /locus_tag="ACHURJ_001204"
     CDS             16283..16528
                     /gene="tusA"
                     /locus_tag="ACHURJ_001204"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFR1280227.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(16630..18828)
                     /gene="zntA"
                     /locus_tag="ACHURJ_001205"
     CDS             complement(16630..18828)
                     /gene="zntA"
                     /locus_tag="ACHURJ_001205"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFR1280228.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIAHQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQSATHPLAQAIVREAQVAALAIPAAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEYKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(18902..19528)
                     /locus_tag="ACHURJ_001206"
     CDS             complement(18902..19528)
                     /locus_tag="ACHURJ_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFR1280229.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYF"
     gene            19669..20028
                     /locus_tag="ACHURJ_001207"
     CDS             19669..20028
                     /locus_tag="ACHURJ_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFR1280230.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(20031..20300)
                     /locus_tag="ACHURJ_001208"
     CDS             complement(20031..20300)
                     /locus_tag="ACHURJ_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFR1280231.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(20290..20886)
                     /gene="rsmD"
                     /locus_tag="ACHURJ_001209"
     CDS             complement(20290..20886)
                     /gene="rsmD"
                     /locus_tag="ACHURJ_001209"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFR1280232.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSLGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            21036..22529
                     /gene="ftsY"
                     /locus_tag="ACHURJ_001210"
     CDS             21036..22529
                     /gene="ftsY"
                     /locus_tag="ACHURJ_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFR1280233.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPVEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLF
                     RGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMG
                     EILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFELQGKSVMLAAGDTFRA
                     AAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSH
                     LMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDG
                     TAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            22532..23200
                     /gene="ftsE"
                     /locus_tag="ACHURJ_001211"
     CDS             22532..23200
                     /gene="ftsE"
                     /locus_tag="ACHURJ_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFR1280234.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            23193..24251
                     /gene="ftsX"
                     /locus_tag="ACHURJ_001212"
     CDS             23193..24251
                     /gene="ftsX"
                     /locus_tag="ACHURJ_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFR1280235.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTNFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            24496..25350
                     /gene="rpoH"
                     /locus_tag="ACHURJ_001213"
     CDS             24496..25350
                     /gene="rpoH"
                     /locus_tag="ACHURJ_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFR1280236.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            25621..26724
                     /gene="livJ"
                     /locus_tag="ACHURJ_001214"
     CDS             25621..26724
                     /gene="livJ"
                     /locus_tag="ACHURJ_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFR1280237.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(26923..27306)
                     /gene="panM"
                     /locus_tag="ACHURJ_001215"
     CDS             complement(26923..27306)
                     /gene="panM"
                     /locus_tag="ACHURJ_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFR1280238.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT         6475 a         7172 c         7675 g         6191 t
ORIGIN      
        1 aacggggaca gttttaggtc ttaacgcccg tgacagtaaa catgctggaa gaatgttcag
       61 catgcataat tgccaaatta accctggtgc agggcctgag ataaccactc cctggaagtc
      121 ttaccagcat gaaaaaaaca gaaatggact tatgcctttg attagaaggc gcacaatgtt
      181 acagctctga accatagaca acatgaatat taatgttact atctggttga gactgttgat
      241 aaaacgtaaa aaggaatgct ctgttcctgc aactgaacag gcgtgaaacg cgatccaaca
      301 ggatggatac cgttttgtca gccagtttta tagatcacac ttatgagtat gcgtgtttca
      361 tttacggcca ggaatgctga tgatgacaac attactaccc gttttcacta agccttcccc
      421 gttggcgctc aatgctctgc gcgctggtcg tatttgtcgt ttccttctta tcccggatgg
      481 aagaatccgc tgaacgtcgt tcggcggcag ttgtaacacg cgtcgtttgc ttacagggat
      541 tatggcgggc cgattatgga taagagtaag cgccatctgg cgtggtgggt tgtcggggca
      601 ctggcggtgg ctgccgtcgt ggcgtggtgg ctgttgcgcc cggcgggtgt gccggaaggc
      661 tttgctgtca gcaatgggcg cattgaagcg acggaagtgg atatcgccag caaaattgcc
      721 gggcgtatcg acaccattct ggtgaaagaa ggccagtttg ttcgcgaagg tgaagtgctg
      781 gcgaagatgg atactcgtgt gttgcaggaa cagcgactgg aagccatcgc gcaaatcaaa
      841 gaggcacaaa gcgccgttgc cgccgcgcag gctttgctgg agcaacgaca aagcgaaact
      901 cgcgccgcgc agtcgctggt taatcaacgc caggcagaac tggactccgt agcaaaacgt
      961 catatgcgtt cccgttcact ggcccaacga ggggctattt ctgcgcaaca gctggatgac
     1021 gaccgcgccg ccgctgagag cgcccgagct gcgctggaat cggcaaaagc tcaggtttct
     1081 gcttctaaag ccgctataga ggcggcacgt accaatatca ttcaggcgca aacccgcgtc
     1141 gaagcggcac aagccactga acggcgcatt gccgcagata tcgatgacag cgaactgaaa
     1201 gccccgcgtg acggacgcgt gcagtatcgg gttgccgagc caggggaagt gctggcggca
     1261 ggcggtcggg tgctgaatat ggtcgatctc agcgacgtct atatgacttt cttcctgcca
     1321 accgaacagg cgggcacgct aaaactgggc ggtgaagcac ggctgatcct cgatgccgcg
     1381 ccagatctgc gtattccggc gaccatcagt tttgtcgcca gcgtcgccca gttcacgcca
     1441 aaaaccgtcg aaaccagcga tgaacggctg aaactgatgt tccgcgtcaa agcgcgtatc
     1501 ccaccggaat tactccagca gcatctggaa tatgtcaaaa cgggcttgcc gggcgtagcg
     1561 tgggtgcggg tgaatgaaga acttccgtgg cctgacgacc tggtggtgag gttgccgcaa
     1621 tgacgcatct ggaactggtt cccgtcccgc ctgtcgcgca actggcgggc gtgagccagc
     1681 attatggaaa aaccgttgcg ctgaacaata tcactctcga tattccggcc cgctgtatgg
     1741 tcgggctgat tggcccggac ggcgtcggga agtcgagttt gttgtcgttg atttccggtg
     1801 ctcgtgtcat tgagcagggc aatgtgatgg tgctgggcgg cgatatgcgc gacccgaagc
     1861 atcgccgcga cgtctgcccg cgcatcgcct ggatgccgca ggggctgggc aaaaacctct
     1921 accacacctt gtcggtgtat gaaaacgtcg attttttcgc ccgcctgttc ggtcacgaca
     1981 aagcggagcg ggaagtgcga attaatgagc tgctgaccag cacggggtta gcaccgtttc
     2041 gcgatcgtcc ggcagggaaa ctctccggtg ggatgaagca aaaacttggg ctgtgctgcg
     2101 cgttaatcca cgacccggaa ctgttgatcc ttgatgagcc aacaacgggg gttgacccgc
     2161 tctcccgcgc ccagttctgg gatctgatcg acagtattcg ccagcggcag agcaatatga
     2221 gcgtgctggt cgccaccgcc tatatggaag aggccgaacg cttcgactgg ctggtagcga
     2281 tgaatgccgg agaagtgctg gcaaccggca gcgccgaaga gctgcggcag caaacgcaaa
     2341 gcgccacgct ggaagaagca tttattaatc tgttaccgca agcgcaacgc caggcgcatc
     2401 aggcggtagt gatcccaccg tatcaacctg aaaacgcaga gattgccatc gaagcgcgcg
     2461 atctgaccat gcgttttggt tccttcgttg ccgttgatca cgtcaatttc cgcattccac
     2521 gcggggagat ttttggattc cttggttcga acggctgcgg taaatccacc accatgaaaa
     2581 tgcttaccgg actgctgccc gccagcgaag gtgaggcgtg gctatttggg caaccggttg
     2641 atccaaaaga tatcgatacc cgccgtcgtg tgggctatat gtcgcaggcg ttttcgctct
     2701 ataacgaact caccgtgcgg caaaacctgg agttacatgc ccgtttgttt cacatcccgg
     2761 aagcggaaat tcccgcgcga gtggctgaaa tgagcgagcg ttttaagctc aacgacgttg
     2821 aagatgttct gccggagtca ttgccgctcg gcattcgcca gcggctttcg ctggcggtgg
     2881 cggtgattca tcgcccggag atgttaatcc tcgatgagcc tacttctggt gtcgatccgg
     2941 tggcgaggga tatgttctgg cagttgatgg tcgatctctc gcgccaggac aaagtgacta
     3001 tcttcatctc cacccacttt atgaacgaag cggaacgttg cgaccgcatc tcactgatgc
     3061 acgccggaaa agtgcttgcc agcggtacac cgcaggaact ggttgagaaa cgcggagccg
     3121 ccagtctgga agaggcattt atcgcctatt tgcaggaagc ggcagggcag agcaacgaag
     3181 ccgaagcgcc gcccgtggta cacgacacca cccacgcgcc gcgtcaggga tttagcctgc
     3241 gccgtctgtt tagctacagc cgccgcgaag cgctggaact gcgacgcgat ccagtacgtt
     3301 cgacgctggc gctgatggga acggtgatcc tgatgctgat aatgggttac ggcatcagta
     3361 tggatgtgga aaacctgcgc tttgcggtgc tcgaccgcga ccagaccgtc agtagccagg
     3421 cgtggacact caacctctcc ggttcccgtt actttatcga acagccgccg ctcaccagtt
     3481 atgacgagct tgatcgtcgg atgagtgcgg gcgatatcac ggtggcgatt gagatcccgc
     3541 ccaatttcgg gcgcgatatc gcgcgtggta cgcctgtgga actcggcgtc tggatcgacg
     3601 gagcgatgcc cagtcgtgcc gaaacggtaa aaggttacgt gcaggcaatg caccagagtt
     3661 ggcttcagga tgtggcgagc cgacaatcga cacccgccag ccaaagcggg ctgatgaaca
     3721 ttgagacgcg ttatcgctat aacccggacg taaaaagcct gccagcaatt gttccggcgg
     3781 tgatcccgct tctgctgatg atgatcccgt caatgctaag cacccttagc gtggtgcggg
     3841 aaaaagagct tgggtcgatt atcaaccttt acgtgacccc caccacgcgt agtgaatttt
     3901 tgcttggtaa acagttgcca tacatcgcgc tggggatgct gaactttttc ctgctctgcg
     3961 gcctgtcggt gtttgtgttt ggcgtaccgc ataaaggcag tttcctgacg ctcaccctgg
     4021 cggcgctgct gtatatcatc attgccaccg gaatggggct gctgatctcc acctttatga
     4081 aaagccagat tgccgccatt ttcggaacgg cgattatcac gttgatcccg gcgacacagt
     4141 tttccgggat gatcgatccg gtagcttcgc tggaagggcc tggacgttgg atcggcgagg
     4201 tttacccgac cagtcatttt ctgactatcg cccgcgggac gttctcgaaa gcgctggatc
     4261 tgactgatat gtggcaactt tttatcccgt tgctgatagc catcccgctg gtgatgggct
     4321 taagtatcct gctgctgaaa aaacaggagg gatgatgcgc catttacgca atatttttaa
     4381 tctgggtatc aaagagttgc gcagtctgct cggtgataaa gcgatgctga cgctgattgt
     4441 cttctcgttt acggtgtcgg tgtattcgtc agcgaccgtt acgccaggat cgttgaacct
     4501 cgcgccgatc gccattgccg atatggatca atcgcagtta tcgaaccgga tcgttaacag
     4561 cttctatcgt ccgtggtttt tgccaccgga gatgatcacc gccgatgaga tggatgccgg
     4621 actggacgcc ggacgctata ccttcgcgat aaatattccg cctaattttc agcgtgatgt
     4681 cctcgccgga cgccagccgg atattcaggt gaacgtcgat gccacgcgca tgagccaggc
     4741 atttaccggc aatgggtata tccagaatat tatcaacggt gaagtgaaca gctttgtcgc
     4801 gcgctaccgt gataacagcg aaccgttggt atcgctggaa acccggatgc gctttaaccc
     4861 gaaccttgat cccgcgtggt ttggcggggt gatggcgatc atcaacaaca ttaccatgct
     4921 ggcgattgta ttgaccggat cggcgctgat ccgcgagcgt gaacacggca cggtggaaca
     4981 cttactggtg atgccgataa cgccgtttga gatcatgatg gcgaagatct ggtcgatggg
     5041 gctggtggtg ctggtggtat cgggattatc gctggtgctg atggtgaaag gtgtactggg
     5101 cgtaccgatt gaaggctcga tcccgctgtt tatgctgggc gtggcgctca gtctgtttgc
     5161 caccacgtca atcggcattt ttatggggac gatagcgcgt tcaatgccgc aactggggct
     5221 gctggtgatt ctggtgctgc tgccgctgca aatgctttcc ggtggatcca cgccgcgcga
     5281 aagtatgccg cagatggtgc aggacattat gctgaccatg ccgacgacac actttgttag
     5341 cctcgcgcag gccattctct accggggtgc cggattcgaa atcgtctggc cgcagttcct
     5401 gacgctgatg gcaattggcg gcgcgttttt caccattgcg ctgctgcgat tcaggaagac
     5461 gattgggaca atggcgtaag gaagggaaac ttgacttcat actgtgatgt catactattg
     5521 atgtcataaa gtgaagtcat aaggacatgc catgagaacc aaggtacagg ctttacggaa
     5581 gaaacaaaaa aatacattgg atcaaatatt taaaactcct gttcctcaag ggatcaagtg
     5641 gtctgatata gagtcactgg ttaaagcatt aggcggagaa attaaggaag gaagaggttc
     5701 gcgttgtaag ttcatactaa atatgagcgt tgcgtgtttc catcggcctc atccgtcgcc
     5761 agataccgat aaaggcgctg tagaaagcgt gcgtgactgg ttactaagta taggggtaaa
     5821 accatgatca aactgaagac gccaaactct atggagattg ccgggcaacc agctgttatt
     5881 acctacgtgc cagaactgaa tgcatttcgt ggcaaattcc tgggcttgtc aggttattgt
     5941 gattttgtct ctgacagcat tcaggggctg caaaaagaag gggaactttc cctgctagag
     6001 tatcttgagg attgtaaagc tgcgggtatt gagccgtatg cgcgtacaga aaagattaag
     6061 acctttacgt tacgctatcc agaatcgctg agtgaacgcc ttaataatgc ggctgcgcag
     6121 caacaagtct ctgttaatac ctatattatt gaaacactga atgagcgttt gaatcattta
     6181 taaattttag cggcgtacat tacgcagaat atctgccttt acaggatcta tctatgcaac
     6241 tgaacttaat tttataaatt aagttcagtt aatatctttt ctttcctcgt ttcagaaata
     6301 actcaatctt ccttcggcaa gcactgcaaa tgcccatgcc gcacgccgcg ctgggcgata
     6361 acgtcatcgg caaaatgctg cacgtcgccc atatcacctt tcagcacggc gatttccaga
     6421 cagtcgtcgt gattgatatg cacatgcagc gtggcgacgg agagatcgtg gtgatggtgc
     6481 tgggtggaga caatgcggct ggctaagtcg cgtttttcgt gttcatacac ataggagagc
     6541 accgcgaaac cttgcgtgcc gtgctgctga gtggcctcct gggccagggc gctacgcaga
     6601 atatcgcgga tagcttcgga acggttgtta taaccacgac gctggctcag gctgtccagc
     6661 gtctccagta aatcgtcatc aagcgtgatg gtgactcgtt gcatttgcgt taaacctttt
     6721 ctgtggcgcg acggcgcacg gggaatgcgg gtaataccgc gttttgtagc acacgtccgg
     6781 cgtcagagga aaaggttaat ttatctccca ccgcctgggt ttcgacgatt tgtccgttgt
     6841 ccatcaccat cacccgctgg caaaagcgtt ctacaagacg taagtcgtgg gtgatgaaca
     6901 ggcaggcggt gccaaactgt tgttgcagct ttttcagcag gcgaatgacg cccgcctgta
     6961 acacgagatc aaggttagaa acggtttcat ccagaatcag caatttcggt tcgaccgcca
     7021 gcgcgcgtgc caggcagacg cgctggagct ggccgccgct taactgcggt gggcgtttgt
     7081 cgagaacgct gtcatcgaga tcgaccgcct tcagcatttc gctggcgcgc gccagttgtt
     7141 cggatttttt cagtgatagc aggtggcgca tcggttcacg caggatctcg cgcacggttt
     7201 tgcgcggatt cacggcactg atggagtcct gaaacaccat ctgaatatca cggcggaagg
     7261 ctttacgttg ggcgcgatta agtttcgcca gcgattcgcc gcgccagcta atattccctt
     7321 gtgagggaga ctctaagcct accagcagcc gcgcgagggt gctttttccg cagccgctgc
     7381 gtcccagcag ggcgacggtt tcgccgcttt tcagggtcag ggaaacgtta ttcagcaccg
     7441 cctgatgttg atgttttccg ctaaatccac cgtgggcata gtgatgggaa aggccgctga
     7501 cgttaagtaa ggtcatgatg ccagctccat accgtagagg gcgagatgag cggaaaccag
     7561 gctgcgcgtc accgtatgtt tgggggcgtt aaacagcgtt tctacatcgc cctgttcgac
     7621 aattttacct tgtgacatca ccgccacgtc atccgccaga cgcgccacaa cgcccatatc
     7681 atgggtgacc agcagcattc ccggcgcttg tttttgcata atgctttcca gcagatcgag
     7741 gatgcgcgcc tgtgctacca cgtcgaggtc ggtggtcggt tcatcggcga tgataaacgg
     7801 tgattcacac agcaccgcca tcgcaatcat catgcgctgc aacatgccgc cgctcatctc
     7861 gaacgggtac agcttcagca cgcgcgcggc gttttccagc cccaccgctt ctatggcagc
     7921 ggtaagcgta gcgtcatcgg cgggtttccc taacgccagg caggtttccc gcgcgtgggt
     7981 gtgcatggtg tgtagcggat taaaggcgct gcgcgggttc tgcatgatgg tggcaatttt
     8041 gatgccgcgc agggcgcagg gataaaccgc tttgccatcg gctaaaattt cccccgccgt
     8101 ctggcgaacg ccagcgggca aaatgcccag cgtcgcggcg caggttaatg atttcccgct
     8161 gccgctaccg ccgactaacg ccagcacgcg cccgcgttgc agggttaacg atacaccgtg
     8221 taccagcggc tgcgcggcct gtagcgcgat attacgtagt tcaatctgtt gcggcattag
     8281 tgtgcgtgct ccgtcaccag atgagggtcc agatgatcgc gcagtgcgtc acccaccagg
     8341 ttaaaggcca tcacgctgat aaacagcgcc agccccggcc agaacatttg cagcggctgg
     8401 gtccagatat actggcgcgc gtcgttaatc atcacgcccc attcggcggt cggcgcggtc
     8461 acaccgaggc caaggaaaga catccccgag acgtgcagca tcatatggcc gatatccagc
     8521 gttgccagca ccagcagcga agggatcacc gcgcctgcca gatgatcgac aaacactcgc
     8581 acatggcccg caccggaaag ccgtgacgcc agcacaaact cgcgttggcg tagtgaaatc
     8641 accaggctgc gcaccatgcg tgcataccac gcccagtgcg acagggcgat ggcgataatt
     8701 acgttggtca gcccggtgcc gagcacgcca accataaaga acgacagaat cgaggtcggg
     8761 aaggtcataa acatatcggc gacgcgcatg gtggcctgat caacgcgccc gccaatcaac
     8821 ccggcgctgc cgccaataac cagccctaat gtcagcacca gcagcaggca ggccattacc
     8881 gaaccgagcg acacgcgggt cgctgccatc agccgcgaga aaatatcgcg acctaagtga
     8941 tcggtgccca gccagtgctg cgcatccggc gaaagcaggt gcgacggcaa atcaatcgcc
     9001 tgggggtcat acggcagcca ccactggctg gtgagcgcaa tcagcgccag cagggcgata
     9061 atgatcagcg ccaggcgtac cgaccagcgg gaagagagga aaaagttcac gcgtgcgctc
     9121 cttcatgacg acgaatgcgc gggtccagcg cggcgttgag caaatcgaca atcaaattac
     9181 agaccacaaa aaccaccacc atcatcagcg taaagcactg aatcaccgga tagtcacggt
     9241 taaaaatcgc cgacaccgca tagcgcccga cgccaggcca ggcaaagatg ttttcgataa
     9301 tcatcgtccc gccaatcaat tcgccaatgt gcatccccac ggcggtgatc atcggcagcg
     9361 aggcattgcg cagaatatgg cggcgttcgg tctgtttgtc attcaggccg cgtagccgcg
     9421 cccaggtgac gtgacgctga ccggcgacgt ccagcatact ggcgcgcagc agtcgcgcgt
     9481 taatcgccag cgacataaag gcgatggaaa ccgcaggcaa aatgatgtgc tgccagccgc
     9541 cgtagcccat cgcgggtagc cattgcagat acaccgaaaa cgccatcact agcaaaaatg
     9601 ccagccagaa gttgggcatc gacacgccaa gaaacgcgat aaagcgcacg gcgaaatccg
     9661 gcaaacgatc gcggtggcgc gccgcccaga taccgagcgg tacggaagtg agcagaatta
     9721 ataccagcgc cgcacctgca agttccagcg tggcgggcag gaagttcagc atatcgtcca
     9781 gcaccgggcg ttggctggcg aatgagatac caaagtcaag atgcagcgcc ttccacaacc
     9841 aggtgccgta ctggacgtac agcggctgat ccagtccgag catggtgcgg gtagaggcca
     9901 gcatctccgg cgtcggcggc aggttagaca gacgcaaata atcgagcgcc gggtcgccgg
     9961 taccgaggcg cagcatcaga aaaatgatca ccgaggcggc gagcaccatc gggatcagca
    10021 gcagaaagcg gcgtaatacg taacgcaaca ttaaggtttc accggtttaa tctgttcgaa
    10081 cggaatttcg gtggcgatcg gcgcgtaggg gatgttaccc agctccggtt ttgataccac
    10141 catcattgag atgtaactga taggcagata aaccgcctcg tcatgcagac gggtcagaat
    10201 gtcgcgatac agtgcctgac gttgcgtttc gtcatgggtc gccagcactt cgccgatctc
    10261 tttatcaatc agcggtttgt cggctaatcc ttgctgtgcc tggaagtcag cgtgcgacgg
    10321 tacgcgcatt gaactcagga aggcgtgtgg atcgtatggc gcgccccagg tgcggtggaa
    10381 aatcatgcca aaacgaccgt cgcgctgacg agcatagata ctgctctctt cttcgccaat
    10441 cagcgagaca tctgcgccaa tctggcgcat atcagcctga atgatttccg ccatcgattt
    10501 gcttaacgca tcggtgccga tgaacgacag ttcaatgcgc agcggctgac catttttctc
    10561 gcggatgtct ttgcccgcag gcagcgtcca accggctttt tccagcaacg ctttcgcttt
    10621 ctgcggatcg tactggcgcg gtttcaggcc gaggttggcg tagggcacag aaggggcaaa
    10681 cagggtgtcg gcgacctgct gggtgccata caacgcgtta tcaatcagcg attttttgtt
    10741 taccgcgtaa ttaagagctt cacgtactgc cagctcattg gtgggggttt tggcggtatt
    10801 aagcgccagc atcacggttt cgatcggctg tgacagttgg gtatggtaag ccgggttctg
    10861 gctaaagcgg gcgaaggtat cgagcggtaa taacccttcg tttccgtaca gcaggtcgat
    10921 atcgccagtt tcaaacgcca ccgcgcgggt agtcgggtcc gggatgacgt taaaggtgat
    10981 ctttttaatc gctggctttt cgccccagta gttttcgtta cggacgaaga catcgtactg
    11041 attcagtttc gattcctgca aaatccacgg tccggtgcca atcggcgctt taattccgtt
    11101 catggtttca tggtttttaa actgcgaggg agcgataaaa cggaaaggac ggggcagggc
    11161 cagttcttgc aggaaaggat agtaggcgct tttcagggta atttgcagct ctgttttact
    11221 gagtgcttta acatcaacaa tctggtttgc cagctccagc caggcgtgac gttgacggtt
    11281 atcgagcact gcgcggaagt tttctgccgc cgcctcggca tcgaacggtt caccgttgga
    11341 taatttcacg tcatcacgca gggtgaaggt ccaggtttta ccatcttctg aatgagtcca
    11401 gctttttgcc agccacggga tcaccgaacc gtctgcctga tatttcacca atggttcata
    11461 aaccatgctc tgggcgaaca tctggttagg cgtgtaaagg tgcgggttta gtggcccgac
    11521 attcaccggc caggcggtag tgatttcatc tggtgcagcg gcatggacga taaaagacgc
    11581 acaagccagc agcgcaaata gagtgcggcg gagtgtggag agcatgataa ccccaatgga
    11641 ttaaaataga tggcggaaat aagtatgacg attttaagta ttcgtcatac tgattacctg
    11701 ttctggatca atagatgggc agtcgggtgg gcggatcagt taactgaatc gatccattgc
    11761 acactgtcgg cggtgagcgt aaagggggtg ggggtgcaga tcgcaaggct taaattttct
    11821 agctgacaat ggctgactga cagcgaggag tgataggtgc tatctacgct gacgatttgc
    11881 caggcgctgc cgccacgctg tttaacgatg gcttctttgc gcgtccagat gcgccagaac
    11941 atttccagtt gctgatcagg atgcacggcg tccatttcag cgtgttcccc gaggctgaac
    12001 acggcgttcg ccagccagcg ccagttggcg cgcgggcgaa tcacttcgat atcgcagccg
    12061 acttcgcctt catcgctcaa cagcagagcg atatcgtcac cgctatggct taagttgaac
    12121 catagcggcg tttccggcgc gaatgcaggt ttgccttgtt cgccatagat gatctccggt
    12181 agcggggaaa gcgtgtgcga aagcaatgca cgccccgcca gccagcgttc gcgtcgtgga
    12241 ccttgcggtg cttgctcgcg taaacccggt ggcagtggag ctgcgcttaa ggtcgaaact
    12301 ttccccagaa ctatccgata catatcaggg ccaacgttta atggaaaatg aaagtgcgta
    12361 tcgtatcact tgtcgcctca tcccggtaac cgacttttcg gtctgcctag ccccagtaaa
    12421 atcgccagtt tgctaccgcc tttggtggtt tccatccaga ttttacacac gctggttaat
    12481 ggcaccgaaa gtagcatccc taccgggccg agcagccatc cccaaattaa caatgaaaga
    12541 aataccacca tggtggacat ccccaggcga tggcccatca tccgtggtcg atgaccatat
    12601 ggaccactaa aaacaatgcg ccgaccagaa tacatgcgta aacaccatta aacagcagca
    12661 cctgaatcat tggcggtacg gcggaaatta ccgcgccgat attgggcacg tagttgagca
    12721 aaaacgccag tactgcccac atcagcgcaa actgtacgcc cattagcgcc agccccagcc
    12781 agacgatgac acctgtccat aaactgagta gcgtctttaa cgccagataa tgcgaaacgc
    12841 ctttaagtgc gcggtgtaat cctgcgatgt gaatctgtgg gttattcagc gcaaaacgca
    12901 ttttgtaagg gacgtggcgc acttcaaaca gcataaaaac tacggtcatc accagcaaaa
    12961 gcacgctcgc cattgccccg gaaagcccga tcattagcgc tgtggtgaag gtaaccactt
    13021 tttccgagtc catccgctgc agcattcgct ccggcgacat atgcaaatta agaaaaggca
    13081 acatctcctg caatttaaaa agtttgcgcg tcagctcctt attaaacttc ggcagcatag
    13141 agataaattc gttaaacgat gccgccagta cgccgactag cgcggttagt gcgatcagca
    13201 tcaccaccac tacaatcgta atggtaacgg ggcgttgtac tccccgacga ataaaccagg
    13261 taacgagcgg gttgaggacg atggcaaaaa acagcgccag taacagctgc acaatgatat
    13321 ctgccgctgc gtgaatgccc gcgaggatca ctaccagcga ggccaacttg agcagaatgt
    13381 gcatgcccgt tttatcgggt tgaggggttt ccattggggc ttccttgtga ctttttgtgt
    13441 taagtgtagc gggagtcacg tcagcaatat tctgattctt actgctgatt ttcacgtcag
    13501 cacgtggtaa aaatgaaaca ctgttgtaaa aatgtggtga tcctcatgcc cgaacccgta
    13561 gccgaacccg cgctaaacgg attgcgcctg aatttgcgca ttgtctccat tgtcatgttt
    13621 aacttcgcca gctacctcac catcgggttg ccgctcgctg tattaccggg ctatgtccat
    13681 gatgtgatgg gctttagcgc tttctgggca ggattggtta tcagcctgca atatttcgcc
    13741 accttgctga gccgtcctca tgccggacgt tacgccgatt tgctgggccc caaaaagatt
    13801 gtcgtcttcg gtttatgcgg ctgctttttg agcggtctgg gatatctgac ggcaggatta
    13861 accgccagtc tgcccgtcat cagcctgtta ttactttgcc tgggacgcgt gatccttggg
    13921 attgggcaaa gttttgccgg aacgggatcg accctgtggg gcgttggcgt ggttggctcg
    13981 ctgcatatcg ggcgagtgat ttcgtggaac ggcattgtca cttacggggc gatggcgatg
    14041 ggtgcgccgt taggcgtcgt gttttatcac tggggcggct tgcaggcgtt agcgttaatc
    14101 attatgggcg tggcgctggt ggccattttg ttggcgatcc cgcgtccgac ggtaaaagcc
    14161 agtaaaggca aaccgctgcc gtttcgcgcg gtgcttgggc gcgtctggct gtacggtatg
    14221 gcactggcac tggcttctgc cggatttggc gtcatcgcca cctttatcac gctgttttat
    14281 gacgctaaaa gttgggacgg tgcggctttc gcgctgacgc tgtttagctg tgcgtttgtc
    14341 ggtacgcgtt tgttattccc taacggcatt aaccgtatcg gcggcttaaa cgtagcgatg
    14401 atttgcttta gcgttgagat aatcggcctg ctactggttg gcgtggcgac tatgccgtgg
    14461 atggcgaaaa tcggcgtctt actggcgggg gcagggtttt cgctggtgtt cccggcatta
    14521 ggtgtagtgg cggtaaaagc ggttccgcag caaaatcagg gggcggcgct ggcaacttac
    14581 accgtattta tggatttatc gcttggcgtg accggaccac tggctgggct ggtgatgagc
    14641 tgggcgggcg taccggtgat ttatctggcg gcggcgggac tggtcgcaat cgcgttatta
    14701 ctgacgtggc gattaaaaaa acggcctccg gaacacgtcc ctgaggccgc ctcatcatct
    14761 taaaacttac tgaataacca gcgtattaat gatgttttct gcggtggtct gcgctttttg
    14821 ctgatcgtca gcgggcagcg taatttgcat ggtcagcagt tgattaccca cgttacccag
    14881 aataacggaa gagtacgccg tctggccttt cgcggagata atactgtcta actgctgcat
    14941 tttgtgacct ttcagctcaa tggctttatt ggttaccact tgcagctgcg gatcgcggct
    15001 acgttgctga tcttccagac gcttcgccag caccgccaga tcttctttcg gatcatcgcc
    15061 catgatgacg atgactgctt tctgcccggt ggcgtcggac cagacatgca tgttattggc
    15121 ctgcgttccc agcttaccgc tctggtcggt catatccgct ggcagcgaga aacttaactt
    15181 gccatcaagc aggttgacgg gattcccggt agcgttattt tccgcgaccg aaccctgcgc
    15241 cgtagcgtta gtgtctttat catcacaggc cgcaagcccc ataaccagca ggccaattcc
    15301 gacatattta accagattgc gcattgactt cttcctttcg ataaacggcc ataacggctc
    15361 attcatccat cttatcacaa ctctgataac gaacctttaa ctcgcctgca aagcgttgat
    15421 ttcggattta tctgccagtc ttttcaacag catattgagt aatacgccat acattggcag
    15481 gaagaaaacg atactgatta acactttgaa acagtaatcg accagcgcga tttccatcca
    15541 gtgttcagcc ataaaggcat ccgggctacg ccagaaggca atgaagaaaa aggccagcgt
    15601 gtcgctgacg ttaccgaaca gtgtggacgc tgtcggtgcc agccaccagc ggcgactctg
    15661 acgcaggcgg ttaaaaacgt gcacgtcgag gatctgtccc agcgcgtagg ccatgaaact
    15721 ggcggtggcg atacgggcga caaacaggtt gaagtgggcg agtgcgccga atccctgcca
    15781 ggaacccata tagaatagcg acgagatgac gtaggagatc agcaacgccg ggatcatcac
    15841 cgcaaagatg atacgacggg ccagcggtgc gccaaaaata cgcacggtca ggtcggtagc
    15901 aaggaaaata aacgggaagc taaacgcgcc ccaggtggta tggaaaccca aaatggagac
    15961 gggaagctga accagatagt tactggaggt gatcaccagc agatgaaata acgataacca
    16021 gaacaacgcc ttatagcgtt gagtttgcga gaaaacgttc atattgtacc tttttgatta
    16081 accattgggg tgagggaacc caatacgtac gacacgtctg ttaccttaac ataataacgt
    16141 gcttaccctt ttttcgagcc gccgcatgat actgctttac gttgacaatg caatggttaa
    16201 ttttcacgca atcgttaacc tggtttgctt acggactcac agggcgtaaa ctagcgccgt
    16261 ttttttatgt gatgagaaga aaatgaccga tctcttttcc agccctgacc acacactcga
    16321 cgcacttggc ctgcgctgcc cggaacccgt aatgatggtg cgcaaaactg tgcgcaatat
    16381 gcagcccggc gaaacgttgc tgattatcgc cgacgatccg gccactaccc gcgatattcc
    16441 tgggttttgt acctttatgg aacacgaact ggttgcgaaa gagacggatg gactgcctta
    16501 tcgttatttg attcgtaaag gcggttgatg ggggctgatt ggcttcgatg ccgccttttc
    16561 ccctcaccct aaccctctcc ccagaggggc gaggggaccg atcgcgctca atgttgcgat
    16621 cggtttgcct tatctcctgc gcaacaatct taacgcattc gctgtcacca gcaccgtcgc
    16681 acccgtatct gccagcaccg ccagccatag tccggtcatc cctaaaagcg tggtgacgag
    16741 gaagatccct ttcagtccca gcgcaatggt gatgttctgg cggatattgg cgtgagtggc
    16801 gcgtgccagt tcaatcattt gcaccaggcc gcgcaggtgg ttatgggtta atgctgcatc
    16861 agcggtttcc agcgccacgt ctgtgccact gcccattgca atcccaatgg cggcagcttt
    16921 catcgctggc gcgtcgttaa taccgtcacc gaccatcgcc agcggcgcat gttgattcag
    16981 ctcggtcacc gctttgactt tatattccgg caacaggccc gctttaaact ccagccccag
    17041 ctccccggca attgccgccg ctgcgcgtgg attatcgccg gtgaggatca cccctttgac
    17101 gcccagcgcg ttcagttcac tgatggcagt tgcagcatcg gcgcgcaggg tatcctgcaa
    17161 tgcaatgata cccagcacgt catcattacg tactaccagc acaaccgttt gcccggcgct
    17221 ttccagttca ttaatcaaac cagcaaatgc atcagcggga tgtttcccgg cagcgcatat
    17281 caacacgcgc tcaccgttaa cctgcgcttc aatgccagac ccgaccagcg cccgctgtga
    17341 ttcggcggcg ggaatggcga gtgcagcaac ctgtgcttcg cgcacgatgg cctgcgccag
    17401 tggatgcgtc gcgctttgct cgaccgccgc cgccagtgtc agcagttcag attcactaat
    17461 acccgttgcc ggatgaatcg cggtaacgcg cggtttaccg acggtcagcg taccggtttt
    17521 atcaaacgcc acctgagtaa cacgacctag ttgttccagc gccgctccgc ctttaatcaa
    17581 cgccccacga cgcgctgctg ccgccagccc ggaggtaatc gccgcgggcg tggagataac
    17641 taacgcacac gggcagccaa tcagcagcag cgtcaggcct ttataaatcc actcctgcca
    17701 gctggcggca aacagcagcg gtggcaccag cgtcaccagc agggcgacgg ccataatcgc
    17761 tggcgtataa atacggctga aacggtcgat aaaccgctca atgggggcgc ggcgctcttc
    17821 ggcttcttcg atcagtttca gaatccggtc aatggcgctg gcacccggtt ctgacagcac
    17881 ttccagcgtt accagacggt ctacgctggt ggctcctgca ggaactttat cgcccgtcgc
    17941 gcgctccacc ggaatggatt ctccggtcag ggcgctttca tcaaaactgg caaacggtga
    18001 gagcagttta ccgtcggcag gcaaacgccc acctgcggcg acttcaatca catcgccagg
    18061 ccgcaggctg ttaatcgcca cctcttcccg ctcaccgtta cgcaggcgcg tggcggtttc
    18121 tggtttcagc gccattaacg cgctaacgcc ctgacgcgcg cggctggcgg cccagccttc
    18181 cagtcgttca ccaatcaaaa acagcagcaa caccatcgca gcttcagccg ttgcgccaat
    18241 aaacagtgca ccaatagcgg ctacgctcat taaggtttca atggcgaagt agctgccgga
    18301 tttgatcagc cgtaatgcct gatgagcaat cgggtacagc ccaaccagcg tggtcgcgat
    18361 aaacgccagt tgcccgaacg gatgattgaa ctgctccaga ccccagctga ttgccatcat
    18421 cacgattagt gtaatcagcg gcagattctc tttcaggcgc gatgcttgtg gctcgtcggc
    18481 ggcctgctca tcgcgcaggg aatagcccgc tttttgcacc gcagattcaa cttgtgcacg
    18541 aatgtcattg tcggcatcaa ccaccagttt ttcggtggcg aacaacacct gcacctgatt
    18601 cacacctgca agctggcgca cggcattttc taccttgcgc gcacaggcgg cgcagtccat
    18661 gccactgact ttccagctat agcgggtgcc ggagacgttt tcagagagcg ttggcgagct
    18721 ggagcatgcg ccgtcgcagc aacagtcgtt ggcgttctgt accgtggtta gcggtttgaa
    18781 cgcagcaaat tgaggggctt tcttgccgtg attgtcaggt gtcgacatgg catactccgg
    18841 ttaagttttt tctcattaac cgaaggatac actctggagt cgactccaga gtcaagtttt
    18901 atcagaaata cagtgagcgg acgatgagga agtgaccggc aaagtagcag gccgcagcaa
    18961 tcgcgttatc cgcacggaag cgtcggcgat agtggctccc cagccagaca aagttactga
    19021 taaacagcaa cgaagcgcca acaaacgcag agagcgccgg agcggtcgga cggaagaacc
    19081 acagttcacc tgccagccac accatcacca gcgtcatgcc gataaacgtg cagataggcc
    19141 aacgatactc ttccagccgc gtccagataa tcgccagtaa cagcgcaccc agaaccagca
    19201 acaccagtgg cagaggccag aagaaagaga gcgtcatctg actggcgaaa tagatggtgt
    19261 acagcaggtg cgagaggaaa aacgcgccga tggcgtacat cagacgttga cgtggcaaca
    19321 gggttagcgc atcgcccagc agtgaggcgc acagccctgc cagcaccaga tagctaatgg
    19381 cgtcgaacat cggcgcttgc caggccagta acagcaggag aagaagggtt aacggtttaa
    19441 acacccagcg ttgccaggtt ggcccacgat acgatgcatc cacagatagc catgcggaaa
    19501 gacagacagc gataaacgac caaagcatct tagttccttg aattcgttat tttttcgtaa
    19561 gcggaatgcc tacggtcttg cttccagttt agtggcgagg gtggacggat gacaacacca
    19621 taactagcag ggtatgctta tttccgcatt ttccgatgag ggataaagat gagcaaacca
    19681 cctcttttct ttattgttat cattggctta attgtcgtcg ccgcatcgtt tcgttttatg
    19741 caacagcgac gggaaaaagc tgataatgat atggctccgc tccagcaaaa gctggtggtg
    19801 gtgagcaaca agcgggaaaa accgattaac gatcgccgtt cgcgccagca ggaagtgact
    19861 ccggcaggca ccagtatgcg ctatgaggca agcttcaaac cgcaaagcgg aggaatggag
    19921 cagacgtttc gcctcgacgc ccagcagtac catgccctga cagtgggcga taaaggtacg
    19981 ctgagctata aaggaacgcg ctttgttagc tttgttggcg aacaataagg ttatttttta
    20041 actttaaatt tcttctgcca gaccagcagt tcaaacacgc cgaaaaggaa aatccgcacc
    20101 ttttcggcgg tggtcatctg cgggccatct ttcggtaaag tggatttcaa tagcgccagc
    20161 tgcataccat gcatcagcac ggtaaaaatc agcgccacgt taacgaaaat attcagcggg
    20221 cgcgggaagg gatgcaccag attgaggatt aaaaatcccc aaacgcagag cattaacaaa
    20281 cgaccaatat taatcagcat cactttctcc ttgtgcttcg cgttgataca gccgataagc
    20341 cacctgaccc gccacttttt cccgatgtaa tgaccagttt gccggaacag tgggcagacc
    20401 gttttcgact tcgctttcga cataaatcag ggcttcgtca gccagccagc cgttatcttc
    20461 cagtaaattt atcgtctctt ctaacaagcc acggcggaac ggtggatcga caaacacgat
    20521 attatgcggt gtaccttttt gcgccaggaa tgacatcgcg ttgctgttca ccacgcgtgc
    20581 attgcctgct tttagtgtcg ccagattctt aattaactgc tgagaaaccg cgcgatccat
    20641 ctcaatcaac gtggcccccg cagcgtagcg cgataacgct tccagtccca gcgcgccgct
    20701 cccggcgaag caatccagac attgggcgtc aacaatgacc ggagccagcc agttaaacaa
    20761 tgtttcgcgt acgcggtcgg tggtggggcg cagacctagg ctatcaggaa ccgggagttt
    20821 acggcctcgc cactgcccgc caataatgcg gatttggccg ctgccggaat gattcggttt
    20881 tttcatgata aattgctcaa tccgccagat aacacatact tgcaggcggt gatgtgaatt
    20941 aagttaagtg atagactatt tcatcatttt tttagctgct atgtacatag cgttaacgct
    21001 gtgccatgaa gcaacagcga ggagtgtagt cgcaaatggc gaaagaaaaa aaacgtggct
    21061 ttttttcctg gctgggcttt ggtcaaaaag agcagacccc ggaaaaagag acagaagttc
    21121 agaatgaaca accggttgta gaagaaatcg ttcaggcgca agagcctgcg aaggcttctg
    21181 aacaagccgt cgaagagcaa ccgcaggcgc atactgaagc cgaggcggaa acttttgctg
    21241 ccgacgttgt ggaagtcact gaacaggttg ctgaaagtga aaaagcgcag cctgaagcgg
    21301 aagtcgttgc acagccggaa ccggtcgtag aagaaacgcc ggagccagtg gctatcgaac
    21361 gtgaagagct gccgttgccg gaagacgtca acgccgaaga ggtttcgcca gaagagtggc
    21421 aggctgaagc ggaaaccgta gagattgtcg aagcggcgga agaagaagcg gctaaagaag
    21481 aaatcaccga cgaagagctg gaagcacagg cgctggctgc cgaagcggca gaagaggcgg
    21541 tgatggtggt tcctccggta gaagagcagc cggtggaaga aatcgctcag gagcaggaaa
    21601 aaccgaccaa agaaggtttc ttcgcgcgcc tgaaacgcag cctgttaaaa accaaagaaa
    21661 atctcggttc cggatttatc agcctgttcc gcggtaaaaa aatcgacgat gatctgtttg
    21721 aggagctgga agaacagctg ttgatcgccg atgtgggcgt ggaaaccaca cgtaaaatta
    21781 tcaccaatct gacggaaggc gcatcccgca agcagcttcg tgatgccgag gcgctctatg
    21841 gcctgctgaa agaagagatg ggcgagattc tggcgaaagt cgatgagccg ctgaatgttg
    21901 aaggtaaaac gccgttcgtg atcctgatgg tgggcgtcaa cggtgtgggt aaaactacga
    21961 cgattggtaa gctggcacgt cagtttgagc tgcagggtaa atcggtgatg ctggcggcgg
    22021 gcgatacttt ccgtgcagca gcggttgaac agcttcaggt ctggggtcag cgcaacaata
    22081 ttccggtgat tgcccagcat accggtgcgg attccgcctc tgttatcttc gacgccattc
    22141 aggcagctaa agcgcgtaat atcgacgtcc tgattgccga taccgccgga cgcctgcaga
    22201 acaaatcgca cctgatggaa gagttgaaga aaatcgtccg cgtgatgaag aaactcgacg
    22261 ttgaagcgcc gcatgaagtt atgctgacta ttgatgccag taccgggcag aatgcggtaa
    22321 gccaggccaa actgttccac gaagccgttg gcttaaccgg catcacgcta acgaaactgg
    22381 acggcacggc gaaaggcggg gtaattttct cggtggctga ccagtttggt atccctatcc
    22441 gctacattgg tgtcggcgaa cgtattgagg atttgcgtcc gtttaaggcg gacgacttta
    22501 tagaggcact ttttgcccga gaggattaac aatgattcgc tttgaacatg tcagcaaggc
    22561 ttatctcggt gggagacagg cgctgcaggg cgttacgttc catatgcagc cgggtgagat
    22621 ggcgtttctg accggtcatt ccggcgcagg gaaaagtacc ctcctgaagc tgatctgtgg
    22681 gattgagcgg cccagcgccg ggaaaatctg gtttagcggc catgacatca cgcgtctgaa
    22741 aaaccgtgaa gttccgtttc tgcgccgcca gattggcatg attttccagg atcaccatct
    22801 actgatggac cgtactgtct acgataacgt ggcgatcccg ctgattatcg ctggtgccag
    22861 cggtgacgat attcgtcgcc gggtgtcggc ggcactggat aaagtcgggc tactggacaa
    22921 agcgaagaac ttccctattc agctttccgg cggtgaacaa cagcgtgttg gcattgcccg
    22981 cgcggtggtg aataagcccg cggtactgct ggcggacgaa ccgaccggta acctggacga
    23041 cgcgttgtcg gaagggattc tacgtctgtt tgaagagttt aaccgcgttg gggtaaccgt
    23101 attgatggca acgcacgaca tcaacctgat ttcacggcgt tcctatcgca tgctcaccct
    23161 gagcgatggt cacttgcatg gaggcgtggg ccatgaataa gcgcgatgca atcaatcata
    23221 ttcggcagtt tggtgggcgt ctggatcgct tccgtaaatc ggtcggcggc tcaggcgacg
    23281 gcggtcgtaa cgcaccgaaa cgcgcgaaat cctcgccaaa accggtaaat cgcaaaacca
    23341 acgttttcaa cgaacaggtg cgctatgcct tccacggcgc attgcaggat ctgaaaagca
    23401 aaccgttcgc cacgttttta acggtgatgg ttatcgccat ctccctgacg ctgcccagcg
    23461 tctgttatat ggtgtacaaa aacgttaatc aggcggcgac gcagtattat ccgtcaccgc
    23521 aaatcactgt ttacttgcaa aaaacgctgg acgatgacgc cgctgcgggc gtggtggcac
    23581 agttgcaggc cgagcaaggc gtggagaaag tgaactatct ttctcgtgaa gacgcactgg
    23641 gcgagttccg taactggtct ggttttggtg gtgcgctgga tatgctggaa gaaaacccgc
    23701 ttccggcagt ggcggtggtg atcccgaaac tcgattttca ggggacggaa tcactcaata
    23761 cgctgcgtga tcgtatcacg cagattaacg gcattgacga agtgcggatg gatgacagct
    23821 ggtttgcccg tctggcggcg ttgaccgggc tggtcgggcg cgtttcggcg atgatcggcg
    23881 tgttgatggt ggcggcagtg ttcctcgtca tcggcaacag cgtacgtctg agcatctttg
    23941 ctcgccgtga ctccatcaac gtgcagaaac tgattggtgc gacggatggt ttcatccttc
    24001 gcccgttcct gtatggtggc gcactgctgg gattttctgg cgcattgttg tcattaattt
    24061 tgtcagaaat tctggtgctg cgattgtcat cggcggttgc ggaagtggca caggttttcg
    24121 gaacgaattt tgatatcaat ggtttatcgt tcgatgaatg cctgctattg ctgctggtat
    24181 gctcgatgat tggctgggtg gcagcgtggc ttgccacggt acaacattta cgccacttta
    24241 cgcctgaata ataaaagcgt gttatactct ttccctgcaa tgggttccgt agcagggaaa
    24301 gagatcccgt tgtctcttcc cggtatttca tctctatgtc acattttgtg cgtaatttat
    24361 tcacaagctt gcattgaact tgtggataaa atcacggtct gataaaacag tgaatgataa
    24421 cctcgttgct cttaagctct ggcacagttg ttgctaccat tgaagcgcca gaagatatcg
    24481 attgagagga tttgaatgac tgacaaaatg caaagtttag ctttagcccc agttggcaac
    24541 ctggattcct acatccgggc agctaacgcg tggccgatgt tgtcggctga cgaggagcgg
    24601 gcgctggctg aaaagctgca ttaccatggc gatctggaag cagctaaaac gctgatcctg
    24661 tctcacctgc ggtttgttgt tcatattgct cgtaattatg cgggctatgg cctgccacag
    24721 gcggatttga ttcaggaagg taacatcggc ctgatgaaag cagtgcgccg tttcaacccg
    24781 gaagtgggtg tgcgcctggt ctccttcgcc gttcactgga tcaaagcaga gatccacgaa
    24841 tacgttctgc gtaactggcg tatcgtcaaa gttgcgacca ccaaagcgca gcgcaaactg
    24901 ttcttcaacc tgcgtaaaac taagcagcgt ctaggctggt ttaaccagga tgaagtcgaa
    24961 atggtggccc gtgaactggg cgtaaccagc aaagacgttc gtgagatgga atcacgtatg
    25021 gcggcacagg acatgacctt tgatctgtct tccgacgacg attccgacag ccagccgatg
    25081 gcaccggtgc tctatctgca ggataaatca tctaactttg ccgacggcat cgaagatgat
    25141 aactgggaag agcaggcggc aaaccgtctg accgacgcga tgcaaggtct ggacgaacgc
    25201 agccaggaca tcatccgcgc gcgctggctg gacgaagaca acaagtccac gttgcaggaa
    25261 ctggctgacc gttacggtgt ttccgctgaa cgtgtacgcc agctggaaaa gaacgcgatg
    25321 aaaaaactgc gcgctgctat agaagcgtaa tttccgctat taagcagaga accctggatg
    25381 agagtccggg gtttttgttt tttgggcctc tacaataatc aattccccct ccggcaaaac
    25441 gtcaatcccc acgcagattg ttaataaact gtcaaaatag ctattccaat atcataaaaa
    25501 tggggtatgt tttagcagag tatgctgcta aagcacgggt agtcatgcat aaaacgaaat
    25561 aaagtgctaa atcacaacac cacaacacac gtaataacca gaagaatggg gattctcagg
    25621 atgaatataa agggtaaagc gttactggca ggatgtatcg cgctggcatt cagcaatatg
    25681 gctctggcag aagatattaa agtcgcggtc gtgggcgcaa tgtccggtcc ggttgcgcag
    25741 tacggtgacc aggagtttac cggcgcagag caggcggttg cggatatcaa cgctaaaggc
    25801 ggcattaaag gcaacaaact gcaaatcgta aaatatgacg atgcctgtga cccgaaacag
    25861 gcggttgcgg tggcgaacaa agtcgttaac gacggcatta aatatgtgat tggtcacctc
    25921 tgttcttcat caacgcagcc tgcgtctgat atctacgaag acgaaggcat tttaatgatc
    25981 accccagcgg caaccgcgcc ggagctgacc gcccgtggct atcagctgat cctgcgcacc
    26041 accggcctgg actccgacca ggggccgacg gcggcgaaat atattcttga gaaagtgaaa
    26101 ccgcagcgta ttgctatcgt tcacgacaaa cagcaatacg gcgaaggtct ggcgcgagcg
    26161 gtgcaggacg gcctgaagaa aggcaatgct aacgtggtgt tctttgatgg tatcaccgcc
    26221 ggggaaaaag atttctcaac gctggtggcg cgtctgaaaa aagagaatat cgacttcgtt
    26281 tactacggcg gttatcaccc ggaaatgggg caaatcctgc gtcaggcacg cgcggcaggg
    26341 ctgaaaactc agtttatggg gccggaaggt gtggctaacg tttcgctgtc taacattgcg
    26401 ggcgaatcag cggaagggct gctggtgacc aagccgaaga actacgatca ggttccggcg
    26461 aacaaaccca ttgttgacgc catcaaagcg aaaaaacagg acccaagcgg cgcattcgtt
    26521 tggaccacct acgccgcgct gcaatctttg caggcgggcc tgaatcagtc tgacgatccg
    26581 gctgagatcg ccaaatacct gaaagcgaac tccgtggata ccgtgatggg tccgctgacg
    26641 tgggatgaga aaggtgatct gaaaggcttt gagttcggcg tatttgactg gcacgccaac
    26701 ggcacggcga ccgatgcgaa gtaatcatta atcggcaact ttgggttgcc gccaaattgc
    26761 taatatcgag tacgttgctt catgccggat gcggcgtaaa cgccttatcc ggcctacaag
    26821 atccaaagaa atcagtaaat tgcaacacac attgtaggcc tgataagcgt agcgcatcag
    26881 gcaatttctc gttggtcatc acttttgaaa tcaacttgac gattaacgct tctcccagcc
    26941 gccctgttgc gccgtaaatc ccagtgcctg cataaacgcc gtcatcacac cgcgatcttc
    27001 cacgcctgca tccgccatcc accagcatga aacgccagga ttgttacgca aaacctcttc
    27061 cagcagatat tgccccacac cgcgacgtcg ggtgacttcc cgcacacgca gggaatccag
    27121 tgctccctcg gtgccgctta aggttacccg tacggcagcg agcaggcgct cgttaaaacg
    27181 cgcggcgtag atacggtggt tatcgtcaac ctgtaaagag gaaggggaat actccggcca
    27241 gatcttttgc aggtcaatcc ggtcctggtc gctaaagttt tctaatcgaa tgatggtcag
    27301 cttcatgggt aacccgtgta aatcacaaaa gtggaaccag tgtagcgaaa taatttaatc
    27361 ggaggctttc tcttttttat ttcttttggc aggtgattaa ttttttaaca gcaataatta
    27421 caaagttaaa acattagaga atgaaaaata cccagcataa tcccctgaat ggtagtgaat
    27481 tattccgccc tttgtgccgt tattttatgc tga
//
LOCUS       DAZPEB010000031        27246 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZPEB010000031 DAZPEB010000000
VERSION     DAZPEB010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27246
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.031"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(175..705)
                     /gene="ppa"
                     /locus_tag="ACHURJ_001216"
     CDS             complement(175..705)
                     /gene="ppa"
                     /locus_tag="ACHURJ_001216"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFR1280239.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            1015..1971
                     /gene="ytfQ"
                     /locus_tag="ACHURJ_001217"
     CDS             1015..1971
                     /gene="ytfQ"
                     /locus_tag="ACHURJ_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFR1280240.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENRIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            2111..3613
                     /gene="ytfR"
                     /locus_tag="ACHURJ_001218"
                     /pseudo
     CDS             2111..3613
                     /gene="ytfR"
                     /locus_tag="ACHURJ_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
     gene            3627..4649
                     /gene="ytfT"
                     /locus_tag="ACHURJ_001219"
     CDS             3627..4649
                     /gene="ytfT"
                     /locus_tag="ACHURJ_001219"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFR1280241.1"
                     /translation="MPQSLPDTTTPKRRFRWPTGMPQLVALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     EVSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            4636..5631
                     /gene="yjfF"
                     /locus_tag="ACHURJ_001220"
     CDS             4636..5631
                     /gene="yjfF"
                     /locus_tag="ACHURJ_001220"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFR1280242.1"
                     /translation="MIKRNLPLMITIGVFVLGYFYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVVGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(5664..6662)
                     /gene="fbp"
                     /locus_tag="ACHURJ_001221"
     CDS             complement(5664..6662)
                     /gene="fbp"
                     /locus_tag="ACHURJ_001221"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFR1280243.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            6838..8211
                     /gene="mpl"
                     /locus_tag="ACHURJ_001222"
     CDS             6838..8211
                     /gene="mpl"
                     /locus_tag="ACHURJ_001222"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFR1280244.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADTANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(8294..8845)
                     /gene="yjgA"
                     /locus_tag="ACHURJ_001223"
     CDS             complement(8294..8845)
                     /gene="yjgA"
                     /locus_tag="ACHURJ_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFR1280245.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEE"
     gene            8939..10291
                     /gene="pmbA"
                     /locus_tag="ACHURJ_001224"
     CDS             8939..10291
                     /gene="pmbA"
                     /locus_tag="ACHURJ_001224"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFR1280246.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGVDCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            10475..10861
                     /gene="cybC"
                     /locus_tag="ACHURJ_001225"
     CDS             10475..10861
                     /gene="cybC"
                     /locus_tag="ACHURJ_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFR1280247.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDTLKVVEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEGKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(10905..11369)
                     /gene="nrdG"
                     /locus_tag="ACHURJ_001226"
     CDS             complement(10905..11369)
                     /gene="nrdG"
                     /locus_tag="ACHURJ_001226"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFR1280248.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(11559..13697)
                     /gene="nrdD"
                     /locus_tag="ACHURJ_001227"
     CDS             complement(11559..13697)
                     /gene="nrdD"
                     /locus_tag="ACHURJ_001227"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFR1280249.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYSNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(14091..15746)
                     /gene="treC"
                     /locus_tag="ACHURJ_001228"
     CDS             complement(14091..15746)
                     /gene="treC"
                     /locus_tag="ACHURJ_001228"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFR1280250.1"
                     /translation="MTNPPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAVLKKVCEFWADRGVDGLRLDVVNLISKDPRFPDDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(15796..17217)
                     /gene="treB"
                     /locus_tag="ACHURJ_001229"
     CDS             complement(15796..17217)
                     /gene="treB"
                     /locus_tag="ACHURJ_001229"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFR1280251.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNARQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAETFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPNYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(17318..18283)
                     /gene="treR"
                     /locus_tag="ACHURJ_001230"
     CDS             complement(17318..18283)
                     /gene="treR"
                     /locus_tag="ACHURJ_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFR1280252.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPEFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLSRSFPER"
     gene            18468..18521
                     /gene="mgtL"
                     /locus_tag="ACHURJ_001231"
     CDS             18468..18521
                     /gene="mgtL"
                     /locus_tag="ACHURJ_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFR1280253.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            18662..21358
                     /gene="mgtA"
                     /locus_tag="ACHURJ_001232"
     CDS             18662..21358
                     /gene="mgtA"
                     /locus_tag="ACHURJ_001232"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFR1280254.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTVVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQLSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(21564..21950)
                     /gene="ridA"
                     /locus_tag="ACHURJ_001233"
     CDS             complement(21564..21950)
                     /gene="ridA"
                     /locus_tag="ACHURJ_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFR1280255.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAIRR"
     gene            complement(22023..22484)
                     /gene="pyrI"
                     /locus_tag="ACHURJ_001234"
     CDS             complement(22023..22484)
                     /gene="pyrI"
                     /locus_tag="ACHURJ_001234"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFR1280256.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(22497..23432)
                     /gene="pyrB"
                     /locus_tag="ACHURJ_001235"
     CDS             complement(22497..23432)
                     /gene="pyrB"
                     /locus_tag="ACHURJ_001235"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFR1280257.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQSELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(23436..23570)
                     /gene="pyrL"
                     /locus_tag="ACHURJ_001236"
     CDS             complement(23436..23570)
                     /gene="pyrL"
                     /locus_tag="ACHURJ_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFR1280258.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(23850..24245)
                     /locus_tag="ACHURJ_001237"
     CDS             complement(23850..24245)
                     /locus_tag="ACHURJ_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFR1280259.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     SYPTWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(24376..25089)
                     /gene="bdcA"
                     /locus_tag="ACHURJ_001238"
     CDS             complement(24376..25089)
                     /gene="bdcA"
                     /locus_tag="ACHURJ_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR1280260.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADKDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMSHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            25160..25753
                     /locus_tag="ACHURJ_001239"
     CDS             25160..25753
                     /locus_tag="ACHURJ_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFR1280261.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGNKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
     gene            25898..26350
                     /gene="tabA"
                     /locus_tag="ACHURJ_001240"
     CDS             25898..26350
                     /gene="tabA"
                     /locus_tag="ACHURJ_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFR1280262.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPHEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPARVRKAVVKMLMA"
     gene            26473..>27246
                     /locus_tag="ACHURJ_001241"
     CDS             26473..>27246
                     /locus_tag="ACHURJ_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR1280263.1"
                     /translation="MSKISDLNYSQHITLADNFKQKSEVLNTWRVGMNNFARNAEGQD
                     NTRNILDPKTFLEFLVKIFTLGYVDFSKRSNEAGRNMMAHIESSSYIKNNDGSEIIKF
                     VMNNPEGERADLSKVEIEITLSTFTTMGTRQGHTAIIFQQPDGSTNRYEGKSFERKDE
                     SSLHLITNKILACYQREANKEIARLLNISQELNNSQDLNNSQVSCKDSVDSTITDLLE
                     KPLNNALLAIRKEHLLLMPYVCNESISYLLGEKGILKEID"
BASE COUNT         6488 a         6782 c         7248 g         6728 t
ORIGIN      
        1 ttccagcaag tggctatgaa cagcaaaatg gtggtcgggt ttgcttgttc ttaccgttca
       61 agccttgcaa ccgctgagaa taatgatggt ggaatagaga gtttatttat cgcgggcata
      121 aaaaaaccct tactaaccga agcccggcgt tcagggttat tgcgctagaa gaacttattt
      181 attctttgcg cgctcgaagg aagcaacgat ttcagcttta gcggcttctg cgttttccca
      241 accttcaact ttcacccact tgcctttttc gaggtctttg tagtgctcga agaagtgagc
      301 gatttgcgct ttcagcagtt caggcagatc gtttacgtct ttaatgtgat cgtattcttt
      361 gctcagcttg ctgtgcggaa ccgcaaccag tttcgcatct tcaccggctt cgtcggtcat
      421 tttcagaacg ccaaccggac ggcaacggat cacagaacca ggctgcagcg ggtacggagt
      481 cgggaccagt acgtcaaccg ggtcaccgtc cagagacagg gtgtggttga tgtaaccgta
      541 gttgcacgga tagaacatcg cggtggacat gaagcggtca acgaacagtg cgccgctctc
      601 tttgtcgatt tcgtatttga tcggatctgc gttagccggg atctcaataa caacgtagat
      661 gtcttccggc agatctttac ccgcagggac gttgagtaag ctcatgtctg tttcctttaa
      721 aaatatgtgg caaacaagtg ccgagtatta tagccaactc gcgccgaatg tctttgcttg
      781 ttttcgtctt cattttcctt tttcgccagt tttaagacgg tatacagagc aggaaaatcc
      841 ataaccgtag ccgcattttt catagtgaga tgaaagcgat tacaaacttg tgattaacgt
      901 tttatttact tttttgaagt gtgatgtaac gcaatccgtt acataacgaa ttgtctatag
      961 ttttttcgcg aacatctttt caccaataat aattaccccg acgaggacaa ccctatgtgg
     1021 aaacgcttac ttgtagtctc tgcagtctcg gcagccatgt cgtctatggc gttggccgct
     1081 ccattaaccg taggattttc gcaggtcgga tcggaatccg gctggcgcgc cgcagaaacc
     1141 aatgtggcga aaagtgaggc cgaaaaacgc ggaattacgt tgaaaattgc cgatggtcag
     1201 caaaagcagg aaaaccggat taaagcggta cgttccttcg ttgcacaagg ggtggatgcg
     1261 atctttatcg ctccagtggt tgcgacgggt tgggaaccgg tattaaaaga ggcgaaagat
     1321 gccgaaatcc cggtcttctt gctcgaccgt tccatcgatg tgaaagacaa atctctctat
     1381 atgaccaccg ttactgccga caacatcctc gaaggtaagt tgatcggtga ctggctggta
     1441 aaagaagtga atggcaaacc atgcaacgtg gtggagctgc agggcaccgt tggggccagc
     1501 gtcgccattg accgtaagaa aggctttgcc gaagccatta agaatgcgcc aaatatcaaa
     1561 attatccgct cgcagtcagg tgacttcacc cgcagtaaag gcaaagaagt catggagagc
     1621 tttatcaaag cggaaaacaa cggcaaaaac atctgcatgg tttacgccca taacgacgac
     1681 atggtgattg gtgcaattca ggcaattaaa gaagcgggcc tgaaaccggg caaagatatt
     1741 ctgacaggtt ctatcgatgg cgtaccggat atctacaaag cgatgattga tggcgaagcg
     1801 aacgccagtg ttgaactgac gccgaatatg gcgggacccg cttttgacgc gctggagaaa
     1861 tacaaaaaag acggcaccat gcctgaaaag ctgacgttaa ccaaatccac cctttacctg
     1921 cctgataccg caaaagaaga gttagagaag aagaaaaata tggggtattg agggttgcta
     1981 tgcctgatgc cgattcgtag gccggataag gcgctcgcgc cgcatccggc gatggtgcac
     2041 tgaagcctga tgcgacgctt accgcgtctt atcatgccta ctgggagcac gctttacacc
     2101 gggggaaacc atgacgaccg accaacacca ggaaatcctc cgcaccgaag gattaagtaa
     2161 atttttcccc ggcgtcaaag cgttagacaa cgttgatttc agcctgcgcc gtggcgaaat
     2221 catggcgctg ctcggtgaaa acggggcggg aaaatcaacg ctaatcaaag cattaactgg
     2281 tgtataccac gccgatcgcg gcaccatctg gctggaaggc caggttatct caccgaaaaa
     2341 taccgcccac gcgcaacaac tcggcattgg caccgtctat caggaagtca acctgctacc
     2401 caatatgtcg gtcgctgata atctatttat aggccgcgaa cccaaacgct tcggccttct
     2461 acgccgcaaa gagatggaaa agcgcgccac cgaactgatg gcatcttacg gtttctccct
     2521 cgacgtgcgc gaaccgctca accgcttttc agtcgcgatg cagcaaatcg tcgccatttg
     2581 ccgggctatc gatctctccg ccaaagtgct gatcctcgat gaacccaccg ccagcctcga
     2641 cacctaggaa gtggagctat tatttggcct gatgcgtcag ttgcgcgatc gcggcgtcag
     2701 cctgatcttt gtcactcact ttctcgatca gagctatcag gtcagcgatc ggatcaccgt
     2761 tttacgcaac ggcagtttcg taggctgtcg ggaaacgcgc gagctaccgc agatcgaact
     2821 ggtaaaaatg atgctggggc gcgagctgga tacccacgcg ctacagcgtg ccgggcgaac
     2881 attgttgagc gacaaacccg ttgccgcgtt caaaaattac ggcaaaaaag gaacgatagc
     2941 accgtttgat ctcgaagtgc gccccggcga gatcgtcggc ctggcaggct tgctgggatc
     3001 cggacgtacc gaaaccgccg aagtgatctt cggtatcaaa cctgctgaca gcggcacggc
     3061 gttgatcaaa ggcaaaccgc aaaacctgcg atcgccacat caggcttcgg tactgggcat
     3121 cggcttctgc ccggaagaca ggaaaaccga tggcatcatc gctgccgcct cggtgcggga
     3181 aaatatcatc ctcgctctcc aggcccagcg cggctggcta cgacctattt cccgcaaaga
     3241 gcagcaagag attgccgaac gctttatccg ccagcttggc attcgcacac cttcaactga
     3301 acaaccgatt gaatttctct ccggcggcaa tcagcaaaaa gtgttgcttt cacgttggct
     3361 actgacccga ccgcaatttc tgatcctcga tgagccaacc cgtggcattg atgttggtgc
     3421 ccacgccgag atcatccgcc tgattgaaac gctatgcgcc gatggtctgg cgctgctggt
     3481 gatctcctcc gaactggaag aactggtggg ctatgccgac cgggtgatca tcatgcgcga
     3541 tcgcaaacag gtggcggaga tccggctggc agagctttcc gttccggcta tcatgaacgc
     3601 cattgcggcg taaggagaac agtgtgatgc ctcaatctct tccggacacc actacgccaa
     3661 aaaggcgctt tcgctggccg acggggatgc cgcagctggt agcactattg ctggtgctgc
     3721 tggtcgacag cctggtagcc ccgcatttct ggcaggtagt gcttcaggat gggcgtttgt
     3781 tcggtagccc catagacatt cttaaccgtg cagcccccgt tgcgttactg gcgattggta
     3841 tgacgctggt gatcgccacc ggtgggattg atctctccgt aggggcggtg atggctatcg
     3901 ccggagccac aacggctgcg atgacggtcg cggaagtcag cctgccgatt gttttgttaa
     3961 gcgccctagg caccggcatc ctggcgggat tgtggaacgg catactggta gcgatcctca
     4021 aaattcagcc atttgtcgcc accctgatcc tgatggtcgc cgggcgcggc gtggcgcaac
     4081 tgatcacctc cggacagatc gtcacgttta actcgccgga tctctcatgg tttggcagtg
     4141 gatcgctgtt gttcctgcca acgccagtca ttattgcggt actgacgctt ctcctgttct
     4201 ggctgttgac ccgcaaaacg gcactgggga tgtttatcga agccgttggt atcaacattc
     4261 gggcggcaaa aaatgccggg gtaaacacgc ggatcatcgt catgctcact tatgtgttga
     4321 gcgggctgtg tgcggcgatt gcgggcatta tcgtggcggc ggatattcgc ggtgccgatg
     4381 ccaacaacgc cggattatgg ctggagctgg acgccattct cgcggtggtg attggcggcg
     4441 gatcgctgat gggcgggcgc tttaacctgc tgctctcggt agtgggggcg ctgattattc
     4501 aggggatgaa caccggaatt ttgctttcgg gctttccacc ggagatgaac caggtggtga
     4561 aagcggtggt ggtgctttgc gtgctgattg tccagtcgca acgctttatc agtctgatta
     4621 aaggagtacg tagccgtgat aaaacgtaat ttgccactga tgatcaccat cggcgtcttt
     4681 gtgctgggct atttctactg cctgacgcag tttcccggtt ttgcttccac aagagtgatc
     4741 tgcaatatcc tgaccgataa cgcttttctg gggattattg ccgttggtat gacctttgtg
     4801 atcctctctg gtggtatcga tctctccgtc ggttcggtga tcgcctttac tggcgtgttt
     4861 ctggcaaaag tggttggcga tttcggcctc tcgccgctgc tggcgtttcc gctagtgctg
     4921 gtgatgggct gtgccttcgg cgcatttatg gggcttctga tcgacgccct gaagatcccg
     4981 gcatttatca ttacgcttgc ggggatgttc tttttgcgcg gcgtcagcta tctcgtttcg
     5041 gaagagtcga ttccgataaa ccatcccatt tatgacacgc tctcaagcct tgcgtggaaa
     5101 atccccggcg gcggtcgcct tagtgcgatg gggctgttga tgctggcggt ggtggttatc
     5161 ggtattttcc ttgcgcatcg tacccgtttt ggcaatcagg tgtacgctat tggcggcaac
     5221 gcgacgtcgg cgaacctgat ggggatttct actcgcagca ctactattcg catttatatg
     5281 ctctccaccg gactggcaac actggcgggg attgtcttct cgatttatac ccaggccgga
     5341 tatgcactgg cgggcgtagg tgtggaactg gacgccatcg cgtcggtggt gattggcggt
     5401 acgttgttaa gtggtggcgt cgggacggtg cttgggacgc tttttggcgt ggcaattcag
     5461 ggactgatcc agacttacat caactttgac ggcacgctga gttcctggtg gacgaaaatc
     5521 gccatcggta ttttgttgtt tatttttata gcattacagc gtgggttaac ggtgctgtgg
     5581 gagaatcgtc agagttcgcc agtgacaaga gtcaacattg cgcagcgata aaaatgcctc
     5641 tccgtgtgga gaggcgcagg agattacgcg tccgggaact cacggataaa gcgttcgaca
     5701 tcttcaacca tatggtcgtt gccgacaaag aacgaacggc gctggtgcag agtttccggg
     5761 atgatatcca gaatacgctc tttgccatcg ctcgctttac cgcccgcttg ttccgccagg
     5821 aatgccatcg ggttgcactc ataaagcaaa cgcagtttgc cgtccgggtg gctggcggtg
     5881 cttgggtaga gataaatacc gcctttcagc aggttacggt ggaaatccgc aaccagtgaa
     5941 ccgatataac gtgaggtata cgggcggttg gtggatttat cttcttcctg gcagaattta
     6001 atgtacttct tcaccccgtt cggaaactta atgtagtttc cttcgttgat ggagtaggtt
     6061 ttgcctttct ccgggaagcg catccgttcc tggcacaggc agaaaacgcc cagcgaagga
     6121 tcgtaagtaa aggcgtgaac accgcatccg gtggtgtaaa ccagcatggt agaggagccg
     6181 tataccacgt aacctgccgc aacctgtttg ttaccaggct ggaggaaatc ttcttccgtt
     6241 accggcgtgc cgacgggcgt aactcggcgg tagatggaga aaatggtacc gacagagacg
     6301 ttaacatcga tgttggacga gccatccagt gggtccatca gcaccacgta ttttgcatgt
     6361 tcacagcctt caaagacaac aatctcatct tcttcttcag aggcaatgcc tgcaacgata
     6421 tcgcgtgctt tcagtgcggc tttcagtttt tcattagcga acaagtcgag tttttgctga
     6481 acctcgccct gcacgttctc agcaccgctg gcacccagga tatcaaccag tcctgctttg
     6541 ttgatatcgc gatggataat cttggcgccc agttttattg ccgacagcaa agcagtgagc
     6601 tcaccggtag catgagaaaa ctcgtgctgc ttttcgacaa taaattcacc taacgttttc
     6661 ataaaacttt ccctgcaatg tttatggagt aaagcgaccg caacaatctt aacaaataat
     6721 ctcaatgttg cgctcaggtg aatcgcgcca gcaaattacg gattatcctg aaatgcgttt
     6781 ctcacttgcc cgacatatgc gtaaaatgag cggcagatta aaaaaggata gtgacgtatg
     6841 cgcattcata ttttaggaat ttgtggcacg tttatgggcg gtctggcgat gctggcgcgc
     6901 cagttaggcc atgaagtaac gggttcggac gccaatgtgt atccgccgat gagcacctta
     6961 cttgagaagc aaggcattga actgattcag ggttacgatg ccagccagct cgatccgcag
     7021 ccggatctgg tgattattgg caacgccatg acccgtggaa atccgtgtgt ggaagcggta
     7081 ctggaaaaaa acatccctta tatgtcaggt ccacagtggc tgcacgattt tgtgctgcgc
     7141 gaccgctggg tgctggccgt tgccggtaca cacggcaaaa ccaccaccgc gggaatggcg
     7201 acctggattc tggaacagtg tggttacaaa ccgggatttg tgatcggcgg tgtgccgggg
     7261 aactttgagg tttcggcgcg tctgggcgaa agcgacttct ttgttatcga agcggatgag
     7321 tacgactgcg ccttcttcga caaacgctct aaatttgtcc attactgccc gcgtacgctg
     7381 atcctcaaca accttgagtt cgatcacgcc gatatctttg acgacctgaa agcgatccag
     7441 aaacagttcc accatctggt gcgtatcgtt ccggggcagg gccgtattat ctggccggaa
     7501 aatgacatca acctgaaaca gaccatggcg atgggctgct ggagcgagca ggagctggtg
     7561 ggtgagcagg ggcactggca ggcgaaaaag ctgaccactg atgcttccga atgggaagtc
     7621 ttgctggatg gcgaaaaagt gggcgaagtg aaatggtcgc tggtaggcga acataatatg
     7681 cataatggcc tgatggcgat tgcggcggcc cgccatgttg gcgtagcgcc ggcagatacc
     7741 gctaacgcgc tgggttcgtt tattaatgct cgtcgccgtc tggagttgcg tggtgaagcg
     7801 aatggcgtca cggtatatga cgattttgcc catcacccga cggcgattct ggcaacgctg
     7861 gcggcgctgc gtggcaaagt tggtggtaca gcgcgcatta ttgctgtgct ggaaccgcgc
     7921 tcgaatacca tgaaaatggg gatctgcaaa gacgatttgg caccttcatt aggtcgtgcc
     7981 gatgaagtct tcctgctgca accggcgcat attccgtggc aggtggcaga agtggcagaa
     8041 gcctgcgttc agcctgcaca ctggagtggc gatgtggata cgctggcaga tatggtggtg
     8101 aaaaccgctc agcctggcga ccatattctg gtgatgagca acggcggttt tggtgggatc
     8161 catcagaaac tgctggatgg tctggcgaag aaggcggaag ctgcgcagta attcggcctc
     8221 agcctgagat agcattgccg gataaggcgt ttacgccgca tccggcaaac catactcaca
     8281 tcaacaacga aaattactct tcattctccg ccagttcgcg cagatactgg aaaatctggc
     8341 gtgcggattt cggcggctta ttcccttctt tctctttctt cgcgttgcgg atcagagtac
     8401 gcagttgctg acgatcggca tcgggccaca gatttaatac ctcagcgatg gcgtcatcac
     8461 cttgatcgat cagacgatcg cgcaggtttt ccagtttatg gaacagcacc acctgctggt
     8521 tgtgacggtt tttcagctta tccagcgcct gacggatcgg ttctacatcg cgctggcgca
     8581 gcatcttacc gatgagctgc aactggcggc ggcgaccttc catcttaata cgctgggcga
     8641 gctcaatagc cgcgcgcaga tccgcatcta acgggatctt atccagcgcg tttttcccca
     8701 gatcaacaat ttccgcgcca aggcgtttta gctcctcggc atcacgttta atttcacttt
     8761 tactgaccca gataatttca tcgtcttcgt cttcgatgtc atcaccggga acgtcgtcga
     8821 gccagtcttc gggctgctta gtcatctcag gctccttaaa aaaagaggct aatgttacca
     8881 gttaagatgc gcactgaaaa acggttctct gttagacttc agagaaactc tctacattat
     8941 ggcacttgca atgaaagtaa tctctcaagt tgaagcgcag cgcaagattc tggaagaagc
     9001 agtttccact gcgctggagt tggcctcagg caaatcggac ggtgcggaag ttgccgtcag
     9061 caagaccacc ggcattagcg taagcacgcg ttatggtgaa gtggagaatg ttgaattcaa
     9121 tagcgatggc gcgctgggga tcactgttta tcaccagaac cgcaaaggta gcgcatcatc
     9181 caccgattta agcccgcagg ccattgcccg tactgtacag gcggcgctgg atattgcccg
     9241 ttatacctcg ccagatccct gtgccggcgt ggcagacaaa gagctgctgg cctttgacgc
     9301 accagatctc gacttgttcc accctgcgga agtttccccg gatgaagcca ttgaactggc
     9361 ggcccgcgca gaacaggcgg cattgcaggc ggacaaacgc atcaccaata ccgaaggtgg
     9421 cagctttaac agccactacg gtgtcaaagt ttttggcaac agccacggca tgttgcaggg
     9481 ttactgctca acgcgtcatt cgctctccag ctgtgtaatt gccgaagaaa atggcgatat
     9541 ggagcgtgat tacgcctaca ccattggtcg tgcgatgagc gatctgcaaa cgccagagtg
     9601 ggttggggtc gactgtgctc gccgtacttt atcgcgcctg tcaccgcgta aactctccac
     9661 catgaaagcg ccggtcattt ttgccaatga agtggcaacc gggctttttg gccatctggt
     9721 gggggcgata gcgggtggat cggtttaccg taaatctacc ttcctgctgg attcgctggg
     9781 taaacaaatt ctgccggact ggctgaccat tgaagagcat ccgcatctgc tgaaagggct
     9841 ggcgtcgacg ccattcgaca gcgaaggtgt gcgcaccgag cgtcgcgata ttattaaaga
     9901 tggcatcctg actcagtggc tgctgaccag ctattcggcg cgaaaactgg ggctgaaaag
     9961 caccggacat gcgggcggta ttcacaactg gcggattgcc ggacaaggtc taagcttcga
    10021 gcagatgctc aaagagatgg gcaccgggct ggtggtgacg gaattgatgg gccagggcgt
    10081 gagtgcaatt accggtgatt attcccgtgg tgcggcgggc ttctgggtag aaaacggcga
    10141 aattcagtat ccggttagcg aaatcaccat cgcaggtaat ttaaaagata tgtggcgcaa
    10201 tattgtcacc gtcggtaacg atattgaaac acgcagtaat atacagtgtg gttctgtgct
    10261 gttgccggag atgaaaatcg ccggacagta aaaaaactgg cgcgacctgt cgcgctataa
    10321 actaaaaaat tacacaaaat cattcgcact gcatcgagac ggaaagtgaa tgagggccgg
    10381 ggagcgtact cgcagtacgt gacccggtcg aatgagcgta gtcgtcgatg aggcagtgtg
    10441 aatgatgaag tgtaaataaa aaaggaagtg agcaatgcga aaaagcctgt tagctattct
    10501 tgcagtctcc tcgttggtat tcagttcggc gtcgtttgcc gctgatcttg aagacaatat
    10561 ggaaaccctc aacgacactt taaaagtggt cgaaaaagcc gataacgcgg cgcaagtcaa
    10621 agacgcgtta acgaagatgc gcgccgcagc gctggatgcg caaaaagcaa cgccgccgaa
    10681 gctcgaaggc aaatcaccgg acagcccgga gatgaaagac ttccgtcacg gtttcgatat
    10741 tctggtcggt cagattgacg acgcgctgaa gctggcaaat gaaggtaaag taaaagaagc
    10801 gcaggctgcc gcagagcaac tgaaaacgac ccgcaacgcc tatcaccaga agtatcgtta
    10861 attcctcatt tccctttgcc tgcactcagg taacagggaa cttatcatcg caaatgatgc
    10921 accacctgat tgctgctgcc gcgccagatc agggaagggt cttttaaatc ctgcacaaat
    10981 ttgccgtcga ccagcacgtt aatcaaatca acaacctgca tttgcgctgc gttgagttcg
    11041 tcgagtttat agcctgtcca cacccagatg tctttacccg gacactcggc gcggatgcgt
    11101 tgtaccagtt tcagaatatc cggcacgttt tgcggatgca gcggatcgcc gccggagagg
    11161 gaaataccct ggcgtttgat acgagtgtca ttcagatcgt taatgatctg gtcttccatt
    11221 gctttagtaa atggctgacc ggaatttacc cgccatgtgc ttttgttata gcaaccgggg
    11281 cattcatgaa cacacccgga gacaaacaga gtgcaacgag tgccggggcc gttgacgatg
    11341 tcgacaggat agtactgatg ataattcatt atttcgcctg gcttatcagt ccgcgtgtag
    11401 actgcatccg gtaatcaatg cctgatgcga cgctgtcgcg tcttatcagg cctacagccg
    11461 ttgccaaatg taggccggat aaggcgttta cgccgcatcc ggcaatcaac gcgggatgcg
    11521 acgctttcac atcacatccg gcatttgctt agtgcagatc aacctatctg cccatttccc
    11581 aaatgtttaa cgcggcgctt aacttcttcc tgcttgccgg cgttaaacgg acgtgcatcc
    11641 gggctaccta aatatccgca cacgcggcga gttaccgaca cacgggaggc gtcatggtta
    11701 ccacatttcg ggcaggtgaa gcctttgctg gtgcactcga actcaccggt aaagccacac
    11761 tcgtagcact catcaatcgg tgtattggtg ccgtaatacg gtacatgctg atagctgtaa
    11821 tcccagacat cttccagcgc cttcaggttg tgctgaatgt ttgaatactc gccgtagcaa
    11881 atgaaaccac cgttcgccag cggcgggtaa ggcgcttcaa agtcgatctt gtcgtacggg
    11941 ttcaccttct tctccacatc gaggtggaaa ctgttggtgt agtaaccttt atcggtcacg
    12001 cccggcacca cgccaaactc agcagtatcg agacggcaga agcggtcgca caggttttca
    12061 ctcggcgtgc tgtagagact gaaaccataa cccgtttctt ccttccactg atccactgcc
    12121 tgacgcagac gttcaacaat ggcgatacct ttcgcgcgaa gctgctcgtt gtcgtaaaca
    12181 tgctcgccgc cgaacagcgc gttaatggtt tcgtggatgc cgatgtaacc cagcgaaata
    12241 gacgcacgac cgtttttgaa gatttcagaa acatcatcgt cagcattgag acgcacgcca
    12301 caagcacctt ccatatagag gatcggggcc acgcgcgctt tcacgccttc gagacgagcg
    12361 atacgggtca tcagcgcctt acgtgccagc accagacgtt catccagcag cttccagaag
    12421 gtggcttcat cgccttttgc ttccagagca atacgcggca ggttcaggct gatcacgccg
    12481 aggttgttac gaccatcgtg gatctgctcg ccgttttcat tttcccacac gccgaggaag
    12541 ctgcggcagc ccatcggagt tttaaacgaa ccggtgactt tcactacctg atcgtagttc
    12601 aggatatccg gatacatgcg cttgcttgcg cactccagcg ccagctgttt gatgtcgtag
    12661 ttcggatcgc cttttttatg gttcaggcca tcgcgaatcg caaacaccag tttcgggaac
    12721 accgcagttt tacggttttt accgaggcct gcgatacggt tacgcaggat agattcctga
    12781 atcaggcgcg actcccagct ggtgcccaga ccaaaaccaa aggttacaaa cggcgtctga
    12841 ccgttggcgg tgtgcagggt gtttacttcg tactccagcg actggaaggc atcgtagcac
    12901 tctttaatgg tacgagagtt agcgtagcct tcggcgtccg ggatgttcca ctcttctgcg
    12961 gtcttgcgat gtttgttgta gctggcggtg acaaacggtg ccagcacttc atcgatacgg
    13021 ttaatggtgg tgccgccata aatatggctg gcgacctgag caataatctg cgcagttaca
    13081 gcggttgccg tagagatcga cttcggcggt tcaatctcgg cgttccccat cttaaagccc
    13141 tgggtcagca tgcctttcag gtcgatcaac atgcagttga acatcgggaa gaacggcgag
    13201 taatcgagat cgtgatagtg aatatcgcca cgctcatgtg cctgcaccac gtcacgcggc
    13261 agcaggtgct gacgtgcata gtgtttagcc acgatcccgg ccagcaggtc gcgctgggtt
    13321 ggaatcacct tgctgtcttt gttggcgttt tcgttgagta acgaggcgtt ggtctgctcg
    13381 accagaccac ggatctcctg attcaggcga ccgcgttttt cacgttcaat gtcgcgatcg
    13441 tgacggtact cgatgtaagc acgagccagt tgtttgtatg gacctgacat cagctggttt
    13501 tcaacagcgg tctggatctc attgatatcc acctggttgc ggccctgcat ctgctcgctg
    13561 acaactgcag caacagtggc gcaataatcg gcatcatcga cttccgctgc tttagctgca
    13621 cgcagaatcg cttctttgat gcgctctgat ttaaacggca ctttgcagcc gtctcgtttc
    13681 atcacatgcg gtgtcatgat cgctccatat ttttaagaac aggttatcca cagaaattgg
    13741 gaaaggcgtt ttccggtttt ttcgtttcgt tttccggtgc tttccgcaat cgccatccgc
    13801 ttttccacaa cgccgcacct ttttcgtggg gagctgtagt agcaattata gtcgattaat
    13861 acaacatatt gggttgggac gcattttaaa gtctatatat agtgctttgc atcaaggatg
    13921 tttgaacttt ttttgatgta gctcaaagta aaaagcagag cgtacggatg acgggcgcta
    13981 cagcgttatg taaatttttt aatgaatttg ctggttgaaa aataaacaaa aacaacatgc
    14041 tgacagacaa aaccccggaa tgaccggcat taccggggct tagggaagat ttacttctgt
    14101 aaccaccaga cagcctcaaa aggccgtaaa ttcatggcac agggttgtgg tgaggcttct
    14161 tcgtagttat gcatcacaag ctgccagttg ccgcgcattt gccctggctg ccagggttgg
    14221 atctcacggc taaggttggc aatgaccagc aaggtttgcc ccttccattc acggcgatag
    14281 caccacaata cagggctgtt tggcagcaga tcctggtaat tgccccatgt caggatggct
    14341 tcctgcttac gcagtgcgat taacttttgg taggtgtaaa acaccgagga atcatcggcc
    14401 agcgcggctt ctacgttgat ttgttgatag ttatcaccca ggccaatcca cggttcgcca
    14461 gccgtaaatc cggcattatc gccgttgctc cattgcatgg gcgtgcggct gttgtcacgg
    14521 gatttactgg cgaggattgc caataactcg tcggcatcac gaccatcgtt gcgcagctcg
    14581 gcaaacatat tgaggctctc tacgtcgcga tagtcagtaa tgcgcgtgaa atgcgggttg
    14641 gtcatgccaa tctcttcgcc ctggtagata tacggcgttc cctgcatgcc atgcagcacc
    14701 atcgccagca tttttgccgc aggcacgcgg tattcacctt catcaccaaa gcgagaaaca
    14761 atgcgcggct gatcgtggtt acaccagaac aaggcattcc atgctacgtt gtgcattcct
    14821 tgttgccagt ggcggaacaa tgttttcaac gccacaaagt caggtttagc cagcgtccat
    14881 ttttcaccac cgggataatc gaccttcagg tgatgaaaat taaaggtcat cgacaattca
    14941 ctgcctgtca gtgccgcgta tcgctggcaa tgctcaaggc tggtggagga catttcacct
    15001 acggtcatta acccgcgtgg cgtaaacaca tcgcggttca tctcgtgcaa aaactcgtgt
    15061 gctcgtggcc cgtcggtgta gaagcgacgc ccgtcgccat ccaggtcatc agggaaacgc
    15121 gggtctttgg agatcagatt caccacatcc aggcgcaacc cgtcgacccc acgatcggcc
    15181 cagaactcac agactttttt cagcactgcg cgtaccgctg gattctccca gttgagatcc
    15241 gcctgttctg gtgcaaagag atgcaaatag tactgttcgc tttccgcatg ccagcgccac
    15301 gcactaccgc caaattttga acgccagttg ttcggtggcg tttctggttc tccatcgcgc
    15361 cagatataaa actggcggta agggctttct ttgttcagcg cctcgcgaaa ccaggcatgt
    15421 tgggtagagg tatggttaaa caccatatcg agaatgatac gaatcccgcg cgattttgcc
    15481 tgtgtcacca gttcgtcaaa atcgtccagc gtgccgtagg tgggatcaat cgccgtatag
    15541 ttcgctacgt cgtaaccgtt atcgacctgg ggagagacat aaaagggggt tagccagatg
    15601 gcatcaacgc ccagtttatg cagatagtcc aggcgttgga taacgccacg taaatcgccg
    15661 gtaccgctac ccgtggtgtc ctgaaaactc tttggataaa tctggtagat aacgccgttt
    15721 tgccaccagt ggggaggatt agtcattacg ttattcctgc aaatgcgagg gggcgcaatt
    15781 gcgccccgaa gaaaattaaa caatgtccag cgtgcccagg cggtatttcc gctgatagat
    15841 aaacgaggtg agtacaatcg ggatgatgat ggcgatagcc attgccagcg caaacacctg
    15901 ccagtagttc ggttgaatcg agagaattcc cggcaggccg cctacgccga tgccattcgc
    15961 cataacgccg ttcaggccgc atagcaatcc tgccagacca gaaccaatca tcgcgcacag
    16021 catcgggaag cgatatttca ggttgatgcc gtacattgca ggctcagtga ccccaagcca
    16081 ggcggagatc gccgcaggca cggagatctc gcgttcattg tgcttgcggc tggaaatgat
    16141 aatgccgatc acggcggagc cctgagcgat attcgacagc gcaatcagtg gccacactgg
    16201 cgtaccaccc atgctttgaa tcatctgcaa atcaatagca agcgtggtct ggtgtacccc
    16261 ggtgatcacc agcggggcgt acaggaagcc aaacaatgcc gcgccaatcg gagcaaagct
    16321 gccggtcatc aggtgacgta ccgcaaaggc aacgccatcg ccaatcatgc gaccaaacgg
    16381 accaatcagc gcatgggcga ggaacaccgc gaggatcagc gaacagacgg gcaccaccac
    16441 cagatagagg taatctggca cgatgcgttt aaggcgagtt tcaataacgc ccagcgccag
    16501 cccggctaac agtgccggga tcacctgcgc ctgatagccc actttggcga tgctgaacat
    16561 gccaaagtcc cacacttccg gcagctgctg cccgagcaga taagcgttca tcagttgggg
    16621 ggaaaccagt gtcacgccaa gcacgatacc aaggatcggt gtgccgccca tttttttcac
    16681 cgctgaccag caaatcccga ccggcaggta gaagaagatc gcttcaccga tcaaccacag
    16741 aaaatcgtag atcgtttgca gggaagggta catttgcgcc agcgtttgac cgttgctcat
    16801 gggcaaatcg ccgatcacat tgcgaaaacc gaggatcaaa ccgccgctaa tcaacgcggg
    16861 cagcaacggg aagaaggtct ctgcgaaatg agagatcaac tgctcatgcc atttcatatt
    16921 ctgccgggcg gcttttttta cctgctcttt atcaacctgc gcctgtccgg ttgacgctat
    16981 cagtgcttga tagtagtcac ccacgttggt gccaatcacc acctgaaatt gcctggcatt
    17041 ggtgaaacag cctttcacca tggggagttg ctcaatttct ttcggtctgg cattggccgg
    17101 ttggttgagg acaaagcgta ggcgagtaat acagtggctc accgtcgcaa tattgccgcg
    17161 cccgccgacc agttcaatca accgatcgat atccgtttgg tttattttgc tcatcataaa
    17221 gccccatggc agatgacatt tttggttggc tgcagaatat attgcgcgga aaaatttaaa
    17281 aacgggaacg ttcccgaaac gcagcgaaga tcacaattta tcgttcagga aacgatctgg
    17341 acagggtggc ggggatgatg atttgttgcg gttcgctgcg cccggttacc tgtgcgatca
    17401 actggcaggc cgcctggcgt ccagcttcgg cgtaaccggg atctacggtg acgatctccg
    17461 gatggaggaa tttcattaac ggcgtattac cgacgctcgc cagttgcaag gtgtcgatgc
    17521 gttgctcttg caggtattta cttgcgccaa gtgccagcgt gtcggttgcg cacagtaagg
    17581 cggtagtttc aggcgtaatc acttttgcta cgttctcata gccttgcttc atagcaaggc
    17641 ctggcagagc ggcaacggga tgcagtttat gcgctttgca gaacgccagg taggcttcgt
    17701 gacgtcgctt accggtcgtc acgtcactgt gcggcacgcc gagataactg atattacgat
    17761 gcccctggtc atacagccgt tgcatcagga tcttgattgc cccttcgtcg tcataacaga
    17821 ccgaagcaaa gccttttgcg tcacgcgcca gcagaaccag cgatgactgc cagtgggcta
    17881 acatttcttc tgttatgccg gtaaaaccga acagcactac gccgtcgata ttacgccgtt
    17941 tcagcacccc caaatgttcg gcaactaatt gcggggaaaa ctgactttcc atcatgattg
    18001 ggtcgtaacc ttgttcatag aactctggca gcatggtttg aacggcgaga ttttctgaca
    18061 acgaatccag gcgggtaaca atgatggcga ccactttatc gctttgccca cgcatagcgc
    18121 gcgcagagcg ggaaggggaa aatccatgct gattcatcac tgcttcaaca cgctcgcggg
    18181 tgcgctggct cacgccgctt tcgttattca gcacccggga aactgtagat ttccccacgc
    18241 cgcttaagcg cgcgatgtct ttgatggtca gccgattttg catcctgttg tcctgtaacg
    18301 tgttgtttaa ttatttgagc ctaactttac ccgtgcattc agcaatgggt aaagtctggt
    18361 ttatcgttgg tttagttgcc agcaggtatt atatcgccat agatgctacg aatattattg
    18421 gattctcctt attatttgcg gcgctttttt cacttaccgg aggttatatg gaacctgatc
    18481 ccacgcctct ccctcgacgg agattaaaac ttttccggta agcccgtctt ttcacggcgt
    18541 taccggatgc gtaaggccgt gacgttttaa cgtccctgct cagctttatt accttcaggt
    18601 aaggcttcgc cacgcctgaa gacatttctg tactgtttca gacagtgcgg agggactcct
    18661 tatgtttaaa gaaattttta cccggctcat tcgccattta ccttcccgtc tggttcatcg
    18721 tgatccattg cctggcgcgc agcagacagt gaatacggtg gtcccgccgt ccttaagtgc
    18781 gcattgcctg aaaatggcgg tgatgcccga agaagaattg tggaaaacgt tcgacaccca
    18841 tccggaaggg ttaaatcagg cggaagtgga atctgcccgc gaacaacatg gtgaaaataa
    18901 attacccgca caacaactgt cgccgtggtg ggtacattta tgggtctgct atcgcaaccc
    18961 ctttaatatt ttactcacca ttctcggcgc tatttcttac gccacggaag atttatttgc
    19021 tgcgggcgtt atcgcgctaa tggtcgctat ttctacgttg ctgaacttta ttcaggaagc
    19081 acgttccact aaagcggcag atgccctgaa agcgatggtc agcaataccg cgacggtgct
    19141 gcgcgtaatt aacgacaaag gcgaaaatgg ctggctggag atcccgatcg accagctggt
    19201 gcccggcgat attataaaac tggcggcggg ggatatgatc ccggcagatt tacgtatctt
    19261 gcaagcgcgg gatctgttcg tcgctcaggc gtcgttaacc ggtgagtctc tgcccgtgga
    19321 aaaagccgct accactcgcc agccggagca cagcaatccg ctggagtgcg acacgctgtg
    19381 ttttatgggc accaccgtgg tgagcggcac ggcacaagca atggtgattg ctacaggtgc
    19441 caatacctgg tttggtcaac tggcggggcg tgttagtgag caggaaagcg agccgaatgc
    19501 ctttcagcaa gggatcagcc gcgtcagtat gctgctgatt cgctttatgc tggtgatggc
    19561 accggtggtg ctgttaatca atggctacac caaaggcgac tggtgggaag cggcgctgtt
    19621 tgcgctttcg gtcgcggtag gcctaacgcc ggaaatgttg ccgatgattg ttacctcgac
    19681 gctggcgcgc ggcgcagtaa agctgtcgaa acagaaagtg atcgtcaaac atctggatgc
    19741 tattcagaac tttggcgcaa tggatattct gtgcactgat aaaaccggca ccctgacgca
    19801 ggataaaatt gtgctggaga atcataccga tatctccggt aaaaccagcg aacgcgtgct
    19861 gcatagcgcg tggttgaaca gtcattatca gaccggactt aaaaacctgc tcgatacagc
    19921 ggtgctcgaa ggtacggatg aagagtcagc gcgctcgctg gccagtcgtt ggcaaaaaat
    19981 cgatgagatt ccgtttgatt tcgagcgtcg ccggatgtcg gtggtagtgg cagaaaatac
    20041 cgagcaccat cagctggttt gcaaaggtgc attgcaggaa atcctcaatg tgtgttcgca
    20101 ggtgcgtcac aatggcgaga ttgtgccgct cgatgacatc atgctgcgta agattaagcg
    20161 ggttactgat acgctgaatc gtcaggggct gcgcgtggtt gcggtggcga cgaaatacct
    20221 gccagcgcgt gaaggagatt accagcgggc ggatgaatcc gacctgatcc tcgaaggata
    20281 tattgctttt cttgatccgc caaaagagac aaccgctccg gcactgaagg cattaaaagc
    20341 gagtggaatt accgtaaaaa tccttactgg cgacagtgag ttagttgcag cgaaagtgtg
    20401 ccatgaagtg gggctggacg cgggagaggt ggtcattggt agtgatattg aaacgctatc
    20461 tgacgacgaa ctggcaaatc ttgcacagcg caccacgctg tttgcccgcc tgacgccgat
    20521 gcataaagaa cgcattgtga ccttactgaa gcgcgaaggg catgtggttg gctttatggg
    20581 cgatggtatt aatgatgcgc ccgccttacg cgcggcggat atcggcattt ctgtggacgg
    20641 cgcggtagat attgcccgtg aagcggctga tatcatcctg ctggaaaaaa gcctgatggt
    20701 gctggaagag ggggttattg agggacgtcg cactttcgcc aacatgctga aatacatcaa
    20761 aatgacggcg agttctaact ttggtaatgt gttcagcgtg ctggtggcga gtgctttctt
    20821 gcccttcctg ccgatgttgc cgttacactt gcttattcag aacctgctgt acgatgtgtc
    20881 acaggtggcg atcccgtttg ataacgtcga cgacgagcaa attcaaaagc cgcagcgttg
    20941 gaatccggcg gatctggggc gctttatgat cttcttcgga ccgatcagct cgatcttcga
    21001 tattctgacg ttttgcctga tgtggtgggt attccatgcc aacacgccgg aaacgcaaac
    21061 gctgttccag tcgggatggt ttgtggtggg cttactgtcg caaacgctga ttgtgcatat
    21121 gatccgcacc cgccgtgtgc cgtttattca gagctgtgca tcgtggccgt taatgatcat
    21181 gaccgtgatc gtgatgattg tcgggatcgc attgccgttt tcaccgctgg ccagttatct
    21241 gcaattacag gcgttgccgt taagctattt cccgtggctg gttgcgattc tggcagggta
    21301 tatgacgtta acccagttgg tgaaaggatt ttatagccgt cgttacggct ggcagtaaag
    21361 aataaactag gcatggtggc ccggattcag attgcagaca aagtgagttt tgttcatgcc
    21421 ggatgcggtg taaacgccta atccggccta caaattcaat caattgcagg aatcacgtag
    21481 gcctgataag cgtagcgcat caggcgattt tgcgtttgtc atcagactta atccgggcat
    21541 gatagcccgg atttccatca agattagcga cgaatagcga tcgcttcgat ctcaatcttt
    21601 acgtctttcg gcagacgggc aacttcaacg caagaacgtg ccgggaaggt ggcgttgtgt
    21661 tcggtgaaga aggcttcgta agtggcgttt acggttgcga agtcgttcag atcttttaca
    21721 aacacggtag ttttaacgat gtcgcccact ttcagaccag cggcttcgac gatcgctttt
    21781 acgttatcca gcgactgacg tgcctgtgca gcgacgtctg ccggtacttc gcccgttttc
    21841 ggatttaccg ggatctgacc ggaggtgatg atcatatttc ccagatcaac gccctgtacg
    21901 taaggaccga tagctgctgg tgcattttcc gtcgcgatag ttttgctcat gatttctcct
    21961 ttattacagc ggtaaaaagt ctggctcatt atagggagcc agacttttat taccaaccgc
    22021 aattaattgg ccagcaccac attatgggaa aactcttttt cacagtattt gcatttgagc
    22081 gcgatgtcat tggcgcgttt tcgcacggca aagctggatg aaaccggttc ggcatggctg
    22141 atacagttgc tgttcgggca gaccaacaca ttgtcgatgc gctctggcag actggggcgc
    22201 gatttaccca ccacttcata gttgtcgata cggttaaccg tggcttgcgg cgcatacaat
    22261 gccagttgat ctacctgatc ttcactcaaa aaggtatttt cgattttgat cagatctttg
    22321 cggcccatct cgccagaagg caggttcaga ccaatggtga tgcgctgatc cgtttcggtc
    22381 agcttgaaca gactcaacag cttaaaaccg atctgggcgg ggatatggtc aattaccgtg
    22441 ccgcgtttaa tagcttcaac ctgcaattta ttatcgtgtg tcatctctat ttccccttac
    22501 agtaccagat cgcgattcag aaccagtgcc agtaacgcct ggcgagcgaa aatcccgttg
    22561 cctgcctgct ggaagtacca ggcgtgtggc gttttatcaa catccgtcgc aatttcatca
    22621 acacgcggca gcggatgcag tactttcata ttggctttgg cgttgtggag atcgctggcg
    22681 cgaagaacaa actgcgcttt cacgttggcg tattcggatg ggtccaggcg ctctttttgc
    22741 acgcgggtca tatacaggat gtctacttcc gccatcactt cttcaataga gctgtgcaga
    22801 ctccatgcga tccctttttc atcgagcata tccagaatgt attgcggcat tgccagcgcg
    22861 tccggcgcga tgaagtaaaa acggttgccg tcgaacttcg ctaacgcctg agtcagggag
    22921 tgaacggtgc ggccatattt caggtcacca accattgcga cgtggagatt gtccagacgc
    22981 ccctgggttt cctgaatagt gaataagtcc agcaaggttt gcgtcggatg ttggttggag
    23041 ccatcaccgg cattcagtac cggtacattg ccggaaaact cggtggccag gcgcgccgca
    23101 ccttcctgcg gatgacgcat cactatcgca tcgacgtaag tgctgataac cgaaatggta
    23161 tcggccagcg tttcgccctt tttacccagt gatgtattgg cgctgtcgga gaagcccacc
    23221 acgctggcac ccaggcggtg catggaagtt tcgaaagaga ggcgggtacg ggtagaggct
    23281 tcgaagaagc agctggcaat gactttgtgc ttcaacagct ctgattgcgg gtttgctttc
    23341 agttttgccg ctgttgccag caccagatta aggtcatcgc gactaaggtc gtttatggaa
    23401 atgatatgtt tctgatatag cggattagcc atcttttatc tcctgacgcc tgggcaaaaa
    23461 aaagcccctc gattgagggg ctgggaatgg gtgatcaacg ggaagaaaaa cggcaggcca
    23521 gcgtcttttt tcagacgcgg taagacaaaa tgtcgaacac actgaaccat acatcctccc
    23581 ggcaaattgt ccggcattat actcatcgtc agaagcggtg caagcaattg atgcaatatt
    23641 ttgtcagcgc aaacggttta tttgaattta aagttaaaat atatgcattt ttatttactg
    23701 tgattctggg gggaactgtc gttcggtatc aataattgca gacatttcct gcttttttct
    23761 ttttcacttt cacgcaatca gatatgcatt ttattccatt catccgactt atagggcgga
    23821 gtgtgaaaac gaacggctag cactattgct tagagctcag ggatgcgcgc tatcacttta
    23881 atttcaaaat caaagcctgc cagccatgta acgcccaccg ccgtccaggt tggataagat
    23941 ggcgcgctaa atatttcatt tttcaccgtc atgatgtctt caaattgttt ttctggatcg
    24001 gtatgaaagc tcgtaacatc aatgatatcg tcaaaagtgc atcccgcagc tgccagggtc
    24061 gcatgcaaat tatcaaaggc cagtctgact tgttgctgaa aatcgggttc tggtgttccg
    24121 tcctctcgac ttcctacttg cccggaaaca aacagcaaat cgccggaacg aatagccgca
    24181 gaataacgat gctcagcata cagtgaatgt cgtccagcag ggaaaacagc ggttctttct
    24241 accatttggt tatcctcaag atttacgaca tgaacagaag atttatattt accaggagcc
    24301 gcttttagcg gacgacgtga gtaaacaaaa cccagacatc atgggaaatg gctgggctta
    24361 attgagcgta gtcggttatg cgccaaacgc gccatcaatg gtatgcatcg cgccggtaac
    24421 aaaactggct tctggccctg ctaaccatgc gaccatacca gcgacctctt ccggttgccc
    24481 atgtcttttg atggccataa aactatgcga catatcgcgc attggcccgt tggcgggatt
    24541 agcgtcggta tcaattggcc ctggctggac gacgttgatg gtgatcccac gcggtccgaa
    24601 atcacgggcc agcccgcgcg ccatgccttg cagggcagat ttactggcgg cataagcagc
    24661 catgcctgca acaggcatac gatcgccatt cacggagccg atgattaaga tgcgtccgcc
    24721 ttcgggcatc tgccgggcgg cttcaacaga ggcatggtaa ggagcatgaa tattgatttt
    24781 gaaaaggcga tcaatatcgt cggcatttaa ttccagggca tcgccaaaga cgccaatacc
    24841 tgcatttacc accaggatat ccaatgcgcc gctcttacga acgacatcaa taacagcgtc
    24901 tttgtcagca ctatctgtga atactgctgt cgctccagtc tcttgtgcca ggcgttcagc
    24961 ggcatctttc gaccccgcat aggtgaatcg tacattggcc ccatcggtga cgaaacgacg
    25021 tacgatagcg gcaccgattc cacggctacc accgaggatg agaactgtct tacctgtaaa
    25081 agcgcccata aggactcctt gatttattat gtaacatgca ttacaaaact gttttaactt
    25141 tctgtcaaca agttttgtaa tggtcactaa aaaacaatct cgcgttccag gtcgtcccag
    25201 acgtttcgct cctgagcagg cagtctctgc ggcaaaagtg ctttttcacc aaaaaggttt
    25261 cgatgctgtc agtgttgctg aagttactga ttatcttggt attaaccccc cgagcctcta
    25321 cgcggctttt ggcaataagg ctgggttatt tagccgtgta ctcaatgaat atgtcggtac
    25381 ggaagctatt ccgcttgccg atattcttcg tgatgatcgt ccggtagggg agtgcctggc
    25441 tgaggtatta aaagaagcgg cgcgccgata tagccaaaac ggcggctgcg ctggctgtat
    25501 ggttcttgaa ggcattcata gtcatgatcc acaagcgcgt gatattgccg ttcaatatta
    25561 tcacgccgca gaaacgacca tttatgacta tatcgccagg cggcatccac aaagcgcaca
    25621 atgtgtgact gattttatga gtaccgtgat gtcagggctt tctgcgaagg cacgggaggg
    25681 gcactccatc gaacaactct gtgcaacagc tgcactggcg ggggaagcga taaaaactat
    25741 tctcaaggag tgatgcgggc cttgacccga aaggcgggaa cgcgcctgcc gataagttgt
    25801 gataagacaa taattcacgc gttaaggcta gcggaattga tcatcttttc gtataacgat
    25861 ggaaatgaaa cgttgtttta attaaggagt ggaaaagatg atcatcggaa atattcataa
    25921 tcttcagccg tggctacccc aggagttacg ccaggcgatt gagcatatca aagcacacgt
    25981 tacggcagaa acgccaaagg gcaagcacga tatcgaaggc aatcgtctgt tttatcttat
    26041 ctcggaagat atgaccgagc cgcacgaagc tcgccgtgcg gagtaccatg cccgctatct
    26101 cgacattcag attgtgttaa aaggtcagga aggcatgacc ttcagcacgc aacctgcagg
    26161 caccccggat accgactggt tagctgataa agacatcgca tttttgccgg aaggcgttga
    26221 tgagaaaaca gttatcctta atgaaggtga ttttgttgtg ttttatccgg gggaagtgca
    26281 taaaccgctg tgcgcagtgg gcgcgccagc ccgggttcgc aaagcagtag tgaagatgct
    26341 gatggcgtga tgacttttcg ccgtaaataa ctccaggttt acggcgagtt tgtgaaaaga
    26401 gcgttttttg atattttttt atgagtaaaa tttgtaatgc ttagacgttc ttattaactc
    26461 aaggagttcg tcatgagcaa aatatcagat ttgaattatt ctcaacacat tacattagcc
    26521 gacaatttta aacaaaaaag tgaagtttta aatacctggc gtgttggaat gaataatttt
    26581 gcccgtaatg ccgaggggca ggataacaca agaaatatcc ttgatcctaa gacatttttg
    26641 gaatttttgg taaaaatatt tactctaggt tatgtggatt ttagcaaacg ctccaacgaa
    26701 gcgggaagaa atatgatggc tcatattgag tcctcatctt atatcaaaaa taatgatggc
    26761 agtgagataa taaagtttgt tatgaataat cctgaagggg aacgagcgga tttatctaag
    26821 gtggaaatag aaatcacact ttccactttc actactatgg ggactcgtca aggacatacg
    26881 gctattatat ttcaacaacc tgatggttcg actaatcgtt atgaaggaaa gtcctttgaa
    26941 agaaaagatg agagttcatt acacctgatt actaacaaga ttctggcatg ttaccaacgc
    27001 gaagctaaca aggaaatagc tcgtctatta aatatttcac aggagttaaa taattctcag
    27061 gacttgaata actcgcaggt gagttgtaaa gattcagttg attctacgat tacggattta
    27121 ttagaaaaac cattgaataa tgcattatta gcaataagga aagaacatct gctattaatg
    27181 ccttatgtat gtaatgaatc gatttcatac ttgcttggtg aaaaagggat actgaaagaa
    27241 atagat
//
LOCUS       DAZPEB010000032        27205 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZPEB010000032 DAZPEB010000000
VERSION     DAZPEB010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27205
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.032"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(37..1335)
                     /gene="eno"
                     /locus_tag="ACHURJ_001242"
     CDS             complement(37..1335)
                     /gene="eno"
                     /locus_tag="ACHURJ_001242"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFR1280264.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(1423..3060)
                     /gene="pyrG"
                     /locus_tag="ACHURJ_001243"
     CDS             complement(1423..3060)
                     /gene="pyrG"
                     /locus_tag="ACHURJ_001243"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFR1280265.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(3288..4079)
                     /gene="mazG"
                     /locus_tag="ACHURJ_001244"
     CDS             complement(3288..4079)
                     /gene="mazG"
                     /locus_tag="ACHURJ_001244"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFR1280266.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4150..4485)
                     /gene="mazF"
                     /locus_tag="ACHURJ_001245"
     CDS             complement(4150..4485)
                     /gene="mazF"
                     /locus_tag="ACHURJ_001245"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HFR1280267.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKRIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(4485..4733)
                     /gene="mazE"
                     /locus_tag="ACHURJ_001246"
     CDS             complement(4485..4733)
                     /gene="mazE"
                     /locus_tag="ACHURJ_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HFR1280268.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(4811..7045)
                     /gene="relA"
                     /locus_tag="ACHURJ_001247"
     CDS             complement(4811..7045)
                     /gene="relA"
                     /locus_tag="ACHURJ_001247"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFR1280269.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7093..8394)
                     /gene="rlmD"
                     /locus_tag="ACHURJ_001248"
     CDS             complement(7093..8394)
                     /gene="rlmD"
                     /locus_tag="ACHURJ_001248"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFR1280270.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     SALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8451..11207
                     /gene="barA"
                     /locus_tag="ACHURJ_001249"
     CDS             8451..11207
                     /gene="barA"
                     /locus_tag="ACHURJ_001249"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFR1280271.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11439..12779)
                     /gene="gudD"
                     /locus_tag="ACHURJ_001250"
     CDS             complement(11439..12779)
                     /gene="gudD"
                     /locus_tag="ACHURJ_001250"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFR1280272.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIMIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGDRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLCARDNAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12800..14140)
                     /locus_tag="ACHURJ_001251"
     CDS             complement(12800..14140)
                     /locus_tag="ACHURJ_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFR1280273.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELHVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14142..15512)
                     /gene="gudP"
                     /locus_tag="ACHURJ_001252"
     CDS             complement(14142..15512)
                     /gene="gudP"
                     /locus_tag="ACHURJ_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFR1280274.1"
                     /translation="MFRSATTSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRAT
                     LSIAGSEMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWS
                     MFTLLQGFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSI
                     FNSAQYFATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQ
                     KELEYIAAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYF
                     FITWFPVYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKT
                     PIVMGMLLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSG
                     GLFNMFGNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIEL
                     KPVAGQ"
     gene            complement(15929..16378)
                     /locus_tag="ACHURJ_001253"
     CDS             complement(15929..16378)
                     /locus_tag="ACHURJ_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFR1280275.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16396..17178)
                     /gene="truC"
                     /locus_tag="ACHURJ_001254"
     CDS             complement(16396..17178)
                     /gene="truC"
                     /locus_tag="ACHURJ_001254"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFR1280276.1"
                     /translation="MLEILYQDEWLVSVNKPSGWLVHRSWLDRDEKVVVMQTVRNQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17178..17507)
                     /locus_tag="ACHURJ_001255"
     CDS             complement(17178..17507)
                     /locus_tag="ACHURJ_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFR1280277.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQHALILAELEKLDA
                     LFADDAS"
     gene            complement(18129..18674)
                     /gene="syd"
                     /locus_tag="ACHURJ_001256"
     CDS             complement(18129..18674)
                     /gene="syd"
                     /locus_tag="ACHURJ_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFR1280278.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18742..19590
                     /gene="queF"
                     /locus_tag="ACHURJ_001257"
     CDS             18742..19590
                     /gene="queF"
                     /locus_tag="ACHURJ_001257"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417274.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFR1280279.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATCGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19702..21066
                     /gene="ppnN"
                     /locus_tag="ACHURJ_001258"
     CDS             19702..21066
                     /gene="ppnN"
                     /locus_tag="ACHURJ_001258"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFR1280280.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            21623..22912
                     /gene="sdaC"
                     /locus_tag="ACHURJ_001259"
     CDS             21623..22912
                     /gene="sdaC"
                     /locus_tag="ACHURJ_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFR1280281.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            22970..24337
                     /gene="sdaB"
                     /locus_tag="ACHURJ_001260"
     CDS             22970..24337
                     /gene="sdaB"
                     /locus_tag="ACHURJ_001260"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFR1280282.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            24449..25204
                     /gene="xni"
                     /locus_tag="ACHURJ_001261"
     CDS             24449..25204
                     /gene="xni"
                     /locus_tag="ACHURJ_001261"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFR1280283.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(25309..26446)
                     /gene="fucO"
                     /locus_tag="ACHURJ_001262"
                     /pseudo
     CDS             complement(25309..26446)
                     /gene="fucO"
                     /locus_tag="ACHURJ_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
     gene            complement(26474..27121)
                     /gene="fucA"
                     /locus_tag="ACHURJ_001263"
     CDS             complement(26474..27121)
                     /gene="fucA"
                     /locus_tag="ACHURJ_001263"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFR1280284.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIAFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCASYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
BASE COUNT         6582 a         7295 c         6553 g         6775 t
ORIGIN      
        1 cggaggctgg catttttaaa tcagataaag tcagtcttat gcctggcctt tgatctcttt
       61 acgaccgttg tacggtgctt tttcgcccag agcttcttcg atacgaatca gctggttgta
      121 tttagcaaca cggtcagaac ggctcataga accagttttg atctggcctg cagcagtacc
      181 aacagccagg tcagcgatgg tagcgtcttc agtttcgcca gaacggtgag agataactgc
      241 agtgtagcca gcatctttcg ccatcttgat tgcagccaga gtttcggtca gagaaccgat
      301 ctggttgaat ttgatcagga tggagttagc gatacctttt tcgatacctt ctttcaggat
      361 cttggtgttg gttacgaaca ggtcgtcacc aaccagctgg attttgtcgc ccagaacttt
      421 ggtctggtat gcgaaaccgt cccagtcaga ttcgtccaga ccgtcttcga tagaaacgat
      481 cgggtactgt ttggtcagtt cttccaggaa gtgagtgaat tcttcagagg tgaacgcttt
      541 gttgccttcg ccagccagaa cgtatttacc atctttgtag aattcagaag ctgcgcagtc
      601 catcgccaga gtgatgtctt tgcccagttc ataaccagca gctttaacag cttcagcgat
      661 aacagccaga gcttcagcgt tggaacccag gttcggcgca tagccacctt cgtcaccaac
      721 agcagtattc atgcctttcg ctttcagaac ttttgccagg tgatggaaaa cttcagaacc
      781 catgcggatg gcttctttca cagttttcgc gccaaccggc tgaatcatga attcctggat
      841 atcaacgttg ttgtcagcgt gctcaccacc gttgatgatg ttcatcatcg gaaccggcat
      901 agagtatttg cccggagtac cgttcagttc agcgatgtgc tcgtacagcg gcataccttt
      961 agcagctgca gcagctttgg cgttagccag agatacagcc aggattgcgt tcgcgccgaa
     1021 tttggatttg ttttcggtgc cgtccaggtc gatcatgatc ttgtcaatgc cagcctgatc
     1081 tttagcatct ttgccaatca gcgcctgagc gatcgggccg tttaccgcag caacagcttt
     1141 ggttacgcct ttacccagga aacgggattt gtcgccatcg cgcagttcca gagcttcacg
     1201 ggaaccagta gaagcacctg acggagcagc tgccataccg acgaaaccac cctccagatg
     1261 tacttcggct tcaacagtcg ggttaccacg ggagtcgatg atttcacgac cgatgatttt
     1321 tacgattttg gacattaggt tttcctcaag tcactagtta aactgaaact ccagacaaac
     1381 aacacgtacc cagggtacgc gttgccgttc taactttttt acttacttcg cctgacgttt
     1441 ctggaactcg ctggcggctt tcacaaagcc tgcaaacagc gggtgaccat cacgtggagt
     1501 agaagtaaac tccggatgga actggcaagc cacgaaccac gggtgattcg gaacttcgat
     1561 gatctcgacc aactgatcat ccccggaacg gcccgcaacg cgcagacctg catcttcaat
     1621 ctgtttcaac agcatgttgt tgacttcgta acggtgacga tgacgctcaa caattgtcgg
     1681 cgcattgtac agctggcgaa ccaggctatc gtcaaccaac tggcactgct gtgcgccgag
     1741 acgcatggta ccgccgagat cgctcttctc gctacgaact tcaacgttgc cgttttcatc
     1801 gcgccactcg gtaatcagcg ccacaaccgg gtacttacag tctggcacaa attccgtaga
     1861 gttggcgttc tccatgttgg caacatggcg agcgtaatca attaacgcca cctgcatacc
     1921 caggcaaatg cccagataag gaatattgtt ctcacgcgca aaacgcgcgg tcgtaatcat
     1981 gccttctacg ccacgatagc cgaaaccgcc aggtacgagg attgcgtcca gacctttaag
     2041 gatttcaacg ccgcgcgttt caacatcttg tgaatcgatc agtttgatgt tgacgctgac
     2101 acgattcttc agcccaccgt gtttcagtgc ttcgatcact gatttataag catccggcag
     2161 ttcaatgtac ttgccgacca taccgatggt gacttcactt accgggttcg cttcttcgaa
     2221 gataacctgt tcccattcgg acagattcgc ttccgggcag tttaagctga atcgtttaca
     2281 aatataatcg tccagcccct gagatttcaa caggcccgga attttataga tggaatcgac
     2341 gtctttcaga gaaataaccg ctttttccgg aacattacag aacaatgcaa tcttcgcacg
     2401 ttcgttcgcc ggaacagcgc gatctgaacg acaaatcagg atgtcaggct ggataccgat
     2461 ggagagcagc tcttttacag agtgctgagt cggtttggtt ttgacttcac cagacgctgc
     2521 catgtacggc accagcgtca ggtgcataaa cagagtgtgc tcacggccaa tttcaacagc
     2581 catctggcga atcgcttcga ggaacggcaa ggattcgata tcacctactg taccgccgat
     2641 ttctaccagt actacgtcat gaccttcgcc accttccagc acgcgctctt tgattgcgtt
     2701 agtgatgtgc ggaataacct gcacggttgc gccgaggtag tcaccgcggc gttctttacg
     2761 cagaacgtca gagtagatac gacccgtggt gaagttgttg cggcggctca ttttggtacg
     2821 aatgaaacgc tcgtagtgcc ccaggtccag gtcggtttca gcgccgtctt cagtaacgaa
     2881 cacttccccg tgttggattg ggctcatagt acctggatcg acgttgatgt acggatccag
     2941 tttcatgatg gtcacattga ggccacgggc ttcaagaatg gctgcgaggg aggctgcggc
     3001 aatgccttta cccagagagg atacgacccc gccggtcaca aaaatatagt tcgttgtcat
     3061 gctgaacctg agaagttagg ttgaaaagac gatggaataa ccaggacggg aaagcagtat
     3121 acccgaacat gacctgtgcc acaaactttc attatccctc ctcttcgcca gcgcactatt
     3181 gaaatcagga gtgagaaaat agcccctttg gggtaaatgt ttttgacgca aatcaagcgc
     3241 ttgtcattta aaaaatgaca caaatggcac ttgaccgcgt aattccctta gagatcaatt
     3301 tcctgccgtt ttacctgttg ccagacttct tccattgttt cgaggtcaac acctgtcatt
     3361 tccagtccac gcgcggcaac aatacgctcc acttcgcgaa aacgacgctc gaatttttcg
     3421 ttcgcttttt gcaatgcgat ttctgctttc gtccctaaat ggcgagccag attaaccgtg
     3481 gcaaacagca ggtcccccat ttcctcctcc agtttagcct ggtcgacaac agcctgccgc
     3541 gcttcgtaca tcacctcgtc gatctcttcg tagactttat caaccaccgg gccaagcgtc
     3601 gtccagtcga agccaacgtt ggcgcaacgt ttctggattt tttgcgcccg cattaaagcc
     3661 ggtaaactac gaggaatatc gtccagcgcc gaatgctgcg ctttctgcgc gcgctcttcg
     3721 gttttgattt gctcccaacg ggcaagcact tcactactgt tttcggcaga actatcagca
     3781 aaaacatgcg gatggcgacg ctctaattta tcgctaatag cagcgcaaat atcattaaag
     3841 tcaaagcgcc cttcttcctg agccatttgc gcgtaaaaca ccacctggaa tagcagatcg
     3901 cccagttcac cgcgaagatc gtcaaaatct tcacgggcga tggcgtccag cacttcgtag
     3961 gtttcttcaa gggtgtaagg cgcaatggtg gcaaatgtct gctctttatc ccacgggcag
     4021 ccgttttccg gatcgcgcag gcgctgcata atagtgagca aacgatcgat ttgattcatt
     4081 gaattgtcct gaaaattgcg ggtctgtcag gtggaaacct gtgaccagaa tagaagtgag
     4141 ttagtaacac tacccaatca gtacgttaat tttggcttta atgagttgta attcctctgg
     4201 ggcaactgtt cctttcttcg ttgctcctct tgcccgccag gcgattcttt ttacctgatc
     4261 agctaacgct acgccatcac gttcctgacc ggataaaaca acttcgaacg gatatccttt
     4321 tgattgcgtt gtacaaggaa cacacagaca catacctgtt ttgttgttgt acatgaaagg
     4381 actcaggaca acagctggac gatgtccagc ttgctcgcta ccttttgtcg ggtcaaaatc
     4441 aacccaaatc agatcgccca tatcgggtac gtatcggctt accattacca gacttcctta
     4501 tctttcggct ctccccagtc gatattctcg tggaggtttt ccggcgtgat gtcgttgacc
     4561 agttcagcaa gcgtaaatac gggctcttta cgcactggct caataattaa tttgccatcc
     4621 accaggtcaa tcttcacttc atcatcaata ttcagattga gcgcctgcat taacgtagcc
     4681 gggatccgca ccgccggtga atttccccaa cgctttacgc tactgtggat catcaccctt
     4741 tcctcaaacc gctatcatat gtagatacag tatatatcaa tctacattgt agatacgagc
     4801 aaatttctgc ctaactcccg tgcaaccgac gcgcgtcgat aacatccggc acctggttga
     4861 gtttacccag cacgcggccc agcacctgca ggttgtaaat ctcaatggtc atgtcgatgg
     4921 tcgccagttg ctgtttggtg tcgctacggc tggcaacgcc aagtacgttc accttctcgt
     4981 tggcgagaat ggtcgtgata tcacgtaaca acccactacg atcattagcc accacgcgga
     5041 ccaccagcga atatccggcg gagtagctct caccccatac cgcgtcaaca atgcgttctg
     5101 gcgcatgaga gcgcagttcc gccagttgtt cgcaatcggc gcggtgtact gaaataccgc
     5161 gcccctgggt aatgaagccg acaatctcat ctccaggaat cggctggcag cagcgcgcga
     5221 tgtggtgcat caggttgcca acaccttcga ctaccacgcg accgttatct ttactgcggt
     5281 tttgcggcgt gtagcttttt tgctgaagtt gcttcagcgc ggcggcgtcc tgctcttcgg
     5341 cactcggctt attaaattgc gattgcagga agttcaccat ctgattgaga cggatatccc
     5401 cgccaccaat cgccgccagc aactcgtcga catcattaaa gttgtaacgc ggcagcagat
     5461 gtttttctgc ttctttcagg ctgatcccca gatgttccag ctcgtcgtca aggatttgcc
     5521 gcccagccag aatgtttttg tcacggtcct gtttacggaa ccaggcgtga attttcgaac
     5581 gcccacggct ggttgtgacg taaccgaggt ttgggtttaa ccagtcacgg ctggggttcg
     5641 gctgtttctg ggtgataatt tcaatctggt cgcccatctg cagctggtag gtgaacggca
     5701 caatgcgccc gccaattttt gccccgatgc agcggtgtcc gacatcactg tggatgtggt
     5761 aagcgaagtc cagcggcgtt gatcccgcag gcaaatcaac gacatcacct ttcggcgtaa
     5821 agacatacac ccggtcgtca aagacctggc tgcgtacttc gtcgagcatt tcgccggaat
     5881 cagccatctc ttcctgccac gcaatcagtt tacgcagcca ggcaatccgg tcttcatgtc
     5941 ccgaacgtgc gccgccagct gccgcgccct ctttatattt ccagtgcgca gcaacaccca
     6001 actctgcatc ttcatgcatc tgtttggtgc ggatttggat ctcaacggtt ttgccacctg
     6061 gccccagaac cacggtatga atagactgat aaccgtttgg tttcgggtta gcgacataat
     6121 cgtcaaactc atccggcagg tggcgatagt gagtgtgcac tatccccagt gcggcataac
     6181 aatcctgtaa acgctcggcg acaatacgta ccgcacgcac atcaaacagc tcatcgaagg
     6241 cgaggttctt tttctgcatt ttgcgccaga tgctgtagat gtgtttcgga cggccataca
     6301 cttcagcttt aacgccttca gctttcatct cagcgcgcag atgaccgaca aactcttcga
     6361 tatagtgttc gcggtcgaga cggcgttcat gcagcagttt tgcgatgcgt ttgtattcgg
     6421 tcgggtgcag ataacggaag cagtaatctt ccagctccca tttcagttgc ccaatcccaa
     6481 gacggttcgc caacggcgcg tagatattgg tgcactcttt tgccgccagt acgcgttcat
     6541 cttccggcgc atcttttact tcgcgcagat gagcaatacg ctccgccagt ttgatgacca
     6601 cgcagcgaaa atcatcgacc atcgccaata acatccggcg aacgttatcg acctgttcgg
     6661 aggaaacaga atcagtgtgc gtcgctttca gctggcggat cgccgccata tcacgcacgc
     6721 cgtgaataag gttaacgaca gacttaccga cgctctcacg cagcacatct tcgctgacta
     6781 cgttggcatc ggccagaggg aacagcagcg ccgcccgcag cgtgtcaatg tccatactta
     6841 atgtcgagag gatctccacc atctcaacac cacgccacaa taacagactg gcatccggat
     6901 gcccctgcgt ctgctgcaga caatacgccc aggtttcggc taagcactca cacgacttct
     6961 ggctggtaat acccagactt gcgatccatt tttccggatc aaattcacca gccttattga
     7021 tatgtgcact tcttaccgca accattgtcc tctcctttag ggaccagacc tgccgaaatc
     7081 ggcaaatcgt aactatttaa cgcgcgagaa aagtaccatc gattccagat gtcccgtgtg
     7141 tgggaacata tccagcatcg ccagtcgcgc aatggtatat cctgctttta ataacgcttc
     7201 gctatcccga gccagcgttg cagggttaca ggatacataa actatacgaa taggttccag
     7261 ttttataatt tgctgcataa cacctgcggc acctgctcgc gccgggtcca gcaacacttt
     7321 atcgaagccg tttttcgccc acggctgctt tgtgacatct tcttcaagat tttcgtgata
     7381 aaacgtcaca ttctgtaagc cattaagacg cgcattctgc tggccttttt ccaccagcgc
     7441 cggaacacct tctacaccga ccacactggc agcttgtgtc gccaatggca gtgtaaagtt
     7501 gcccataccg cagaacagat ccagtacgcg atcttcaggt tgcacatcca gccattccaa
     7561 cgcactcgct accatttttt ggttcacacc cgcattgacc tgaataaaat cgcgcgggct
     7621 aaaagttaag cgcaacccgt tagagtcata ccagggcatc tcaccagaga cggtttcgag
     7681 tatctcacta tcgggggcga gatacagatc caggccttca gaatgcgaaa agcgttccag
     7741 tttttcgcga tctgccgaac ttagcggtgc ggtatggcgc aaaatcatca gcgtgccgct
     7801 ggttgcctgt accagttcaa catgaccaag atggcgcatt gcttgtaagc tgcccaggca
     7861 tgccctgact ttgggcagca atgcttcaag ttggggcact aaaatgggac attgtttaac
     7921 gtcgacaatg tcactggagc ccgctttgcg aaaccccatc tgaagttgct gtgttttcgg
     7981 taagtagttc agacttaaac gcgcgcgacg gcgatagccc cagggaacat cggcgatcac
     8041 ttcagagact tcgtgtttca ttaatcgggc gagtgccgca cttttgcttc gctgctgtaa
     8101 atccacgctg gcgtgttgtt gctgacagcc gccgcatacg ccaaaatgag gacagcgtgg
     8161 cgtttcgcgt tccgggctat cgcttaaccg gcgtacgact ttagcgcggg catactgttt
     8221 tttatcttca gtaacagtaa cttccgcgtt ttcctgcggc aataatccgg ggataaatag
     8281 cgttttgccg ttatgtcgcg ccacgccctg accaaaagag tcgaggtcgt tgactgaaac
     8341 ggttatgatc tgacgcgtcg tcgtgcgtcg ttttgcagag tagaattgcg ccattggcga
     8401 gactttctca atttaacagt gtgaccttaa ttgtcacata acggaactcc atgaccaact
     8461 acagcctgcg cgcacgcatg atgattctga tcctggcacc gaccgtcctt attggtttat
     8521 tgctgagtat atttttcgtc gtgcatcgct ataacgactt gcagcgtcaa ctggaagatg
     8581 caggtgccag cattattgag ccgcttgcag tttctactga atatggcatg agcctgcaaa
     8641 atcgcgaatc tatcggtcag ttaataagcg tactgcatcg tcgccattcc gatattgttc
     8701 gcgcgatttc ggtttatgat gaaaataacc gactctttgt cacctccaat tttcatcttg
     8761 atccctcatc aatgcagctc ggcagcaacg tgccgtttcc tcgccagctc actgtcactc
     8821 gtgacggcga tattatgatc ctccgcacgc cgattatttc tgagagttac tcccccgacg
     8881 aatcgcccag tagcgatgcc aaaaatagtc aaaatatgtt gggatatatt gcgcttgagc
     8941 tggatcttaa atcggttcgc ttgcagcaat ataaagagat ctttatttcc agcgtgatga
     9001 tgctgttttg tatcggtatt gcgcttattt ttggctggcg cttaatgcgc gatgtaaccg
     9061 gtccgattcg caacatggtg aataccgtcg accgcatccg tcgcgggcaa ctcgacagcc
     9121 gggtggaagg atttatgctc ggcgagctgg atatgctgaa aaacggtatc aactcgatgg
     9181 caatgtcgct ggctgcttat cacgaagaga tgcagcacaa tatcgaccag gcgacgtccg
     9241 atctgcgtga aacgctggag cagatggaaa ttcagaacgt tgagttggat ctggcgaaaa
     9301 agcgcgccca ggaagcggcg cgtattaaat ccgagtttct ggcaaatatg tcacacgagc
     9361 tgcgtacacc actgaatggt gttattggct ttacccgcct gacgctgaaa acagaattaa
     9421 caccaacgca gcgcgatcac ctgaatacga ttgaacgttc ggcaaataat ttgctggcaa
     9481 ttattaatga tgttctcgac ttctcgaaac tggaagcagg taagctgatt ctggaaagta
     9541 ttccattccc actacgcagc acgctggatg aagtcgttac tctgctggca cattcttctc
     9601 acgataaagg gttggaactg acgctcaata ttaaaagcga cgtgcctgat aacgtgatcg
     9661 gcgacccact gcgattacag caaatcatca ctaacctggt ggggaatgca attaaattca
     9721 ccgagaatgg caacattgat attctggtag aaaaacgtgc gctgagtaat accaaagtgc
     9781 agattgaagt gcagattcgg gataccggca ttggtattcc tgaacgcgat caatcgcgct
     9841 tattccaggc cttccgacag gctgatgcca gtatttcccg ccgtcatggt ggcaccgggc
     9901 tggggctggt gattacacaa aaactggtta atgaaatggg cggcgatatt tcgttccata
     9961 gccagccgaa tcgcggttca actttctggt tccacattaa tctcgatctg aacccgaaca
    10021 ttattatcga agggccatcc acccagtgcc tcgcaggtaa gcgcctggcc tatgtcgaac
    10081 caaactccgc agcagcgcaa tgcacgctgg atattttaag tgaaacgccg ctggaagtgg
    10141 tttatagccc aacgttctcc gcgctgcctc ccgcgcatta cgacatgatg ttgttaggca
    10201 tcgcggtgac cttccgcgag ccgctaacaa tgcaacatga gcgattagcg aaagcggtat
    10261 cgatgaccga tttcctgatg ctggcacttc cttgccatgc acaagtcaat gctgaaaaac
    10321 tcaagcaaga tggtatcggc gcgtgtctgc tgaaaccatt aacacctaca cgcctgttgc
    10381 ctgccctgac ggaattttgt catcacaaac aaaacacgct tttgcctgta accgatgaaa
    10441 gtaagctggc aatgacagtc atggcggttg atgacaaccc cgctaacctg aaacttatcg
    10501 gcgcattgct ggaagatatg gtgcaacatg tggaactttg cgatagcggg catcaggcgg
    10561 ttgaacgggc gaaacagatg ccgttcgatt tgatcttaat ggatattcaa atgccggaca
    10621 tggatggcat tcgggcctgc gaactcatcc accagctccc ccatcagcaa caaacgccgg
    10681 ttatcgcggt aacggcgcat gcaatggccg ggcaaaaaga gaagctgctt ggcgcaggga
    10741 tgagcgatta tctggcgaaa ccgattgaag aagagcgatt gcataatttg ttgttgcgct
    10801 acaagcctgg cagcggtatt tcctctcgcg tcgtgacgcc cgaagtcaac gaaattgtgg
    10861 tgaacccgaa tgcgaccctc gactggcaac tggcactacg ccaggcagca ggaaaaaccg
    10921 atttagcgcg cgatatgctg caaatgttac tcgatttcct gcctgaagtt cgcaacaaag
    10981 ttgaggaaca gctggttgga gaaaacccgg aaggcctggt tgatttgatt cataaactgc
    11041 atggcagttg cggctatagc ggtgtgccgc gtatgaagaa tctctgccaa ctgatcgaac
    11101 aacagctacg tagcggtact aaagaagaag atttggagcc ggagctgctg gaactgttgg
    11161 acgagatgga taatgtcgcg cgcgaagcca gcaaaattct cgggtaacgg acgtcttgtc
    11221 gagtagcaat cggcttttta tgacgctggt ttcagattgg tgacaaagtg cctgtgttta
    11281 tgccggatgc ggcgttaacg ccttatccgg cctacaaaac caattaaatt caatgaatta
    11341 aaaaatgatg tagacctgat acatagcaca acaggctatt ttgcgtttag catcagtctc
    11401 aagccggctc cagatagagc cggttttggt tttctgtctt aacgcaccat gcacgggcgc
    11461 ttgttatcga acgtccagcc aggaatcaga tactgcattc ccatcgcatt gtcacgcgcg
    11521 caaagcccgt gtttctgata cagctcatgg gctttcatca cttgatccat atcgatttct
    11581 acacccagcc ccggtttttc cggcacctga accagaccgc ctttgatctc aaacggttct
    11641 ttggtcaagc gctgattgcc ttcctgccaa atccagtgcg tatcaatcgc ggtgatttta
    11701 cccggtgcag cggcggcaac atgggtaaac atcgccaggg aaatatcgaa gtggttgttt
    11761 gagtgtgaac cccaggtcag gccaaattca tggcacattt gcgccacacg taccgaacct
    11821 tgcatcgtcc agaaatgcgg atccgccagc gggatatcaa cggattgcag ggagagcgta
    11881 tggcccatct gccgccagtc ggtggcgatc atattggttg cggtcggcag gccagttgcg
    11941 cgacggaact ctgccatcac ttcacgcccg gagaaacctt gctccgcacc acacggatct
    12001 tctgcataag ccagcgaccc tttcaggtat ttaccaattt taatcgcttc gttcagcgac
    12061 caggcaccgt taggatcgag cgtaatacgc gcctgcggga agcgttgcgc cagtgccaca
    12121 atagactcgg cctcttcttc cccggccagt acaccgcctt tcagtttgaa atcgttgaag
    12181 ccatattttt catacgccgc ttccgccagg cgcaccaccg catccggcgt catcgcttct
    12241 tcatgacgca ggcgatacca gtcgcatgag tcatccggct ggctttgata cggcagcggt
    12301 gtggctttgc gatcaccgac gaagaacaga taaccgagca tttcgacttc gctacgctgt
    12361 tgaccatcgc ccagcaggga tgccacgttt acccccagat gctgccccag cagatccagc
    12421 attgccgctt ctatcccggt aactacatga atagtggtac gcaaatcaaa tgtctgcaaa
    12481 ccgcgcccac cagcatcacg atcggcaaaa gtattacgca ccagcgtcag aacgtttttg
    12541 tattcaccca gcgttttacc taccaccagc ggaatcgcat cttccagcgt tttacggatt
    12601 ttctcgccgc cgggaatttc ccctacgcca gtgtgaccag aattatcttt gataatcata
    12661 atattacgcg taaagaacgg tgcgtgtgca ccactcagat tcatcagcat actgtcatga
    12721 cccgccaccg ggatgacctg catttcagta acaacaggcg tcgtaaattg agaactcata
    12781 tttatgtcct ttttcagaat caatgacggc cgaaaacggg acgtttacgg tcaaaggtcc
    12841 agccggggat caggtactgc atcggacctg cgtcgttacg cgcaccgcca ggcagacgtt
    12901 tataggcctc atgtgccttt tgtacctgtt cccagtccag ttccacgccc agaccgggcg
    12961 catcaggaac ggcaattttt ccgtttttaa tctccagcgg atttttggtc aggcgacaat
    13021 cgccctcctg ccaaatccag tgggtatcga tagcggtagg attacccggt gccgccgcgc
    13081 ccacatgggt aaacatcgcc agagagatat cgaaatggtt attagaatgg cagccccagg
    13141 tcagccccca gtcgtcgcaa agctgcgcca cacggactgc accggaaagc gtccagaagt
    13201 gcggatcggc aagtggaata tctaccgcat tgagcatcac cgcatgaccc atttcgcgcc
    13261 agttggtggc gatcatgtta gtcgcgacgg gcaagccggt cgcccgccga aactccgcca
    13321 taacttcacg accggagaaa ccctgttctg cgccgcatgg atcttcggca taggtaagaa
    13381 catcattcag ccctttgcat agagaaatgg cttcatcaag tagccaggca ccgttgggat
    13441 caacggtaat ccgcgcatca gggaagcgtt tcttcaatgc acgaacagtg tcgatttctt
    13501 gctcgccagg taacaccccg cccttaagtt tgaaatcttt aaagccatag cgatcctgtg
    13561 aggcttccgc cagacgcaca acggcttcgc tgttcatcgc tttctgatgg cgcaactgat
    13621 accactcatg gttgcccggc gtattttcca gataaggaag atcggttttg gtccgatcac
    13681 cgatataaaa cagataaccg aggacggtaa tagtctcgcg ttgcttgcct ggccccaaca
    13741 gttcgcagac cggaacattc agcgccttac ctagcaggtc aagcaaggcg gcttccagcg
    13801 ccgccacggc attaacgtgc aattcaaaag tccaggcacc tttgccgaag gtatcaaaat
    13861 cggctgcctg attaccttta tgcacctgct gaaccacttt attcaggcgc gcaacttcct
    13921 ggcccagaac catcggaata gcatcgacca gtgtttgata aatcacctct ccgcccggcg
    13981 cttcaccaat gccggtatgc ccggcgttat cggtgagtac cacaatattg cgggtgaaat
    14041 atgcgttatg tgccccacca atattaagca acatgctatc gtgcccggcg accggaatga
    14101 ctttcatatc agtaataaca ggactggatt gtgtcgtcat cttattgccc cgcgacaggt
    14161 ttcagctcga tacgcttgat atcgcccacc agcaccaggt agctcagtac cgcgattaag
    14221 gcatgaacac caacataaat cagcgcccca ttaaaggagc cagtcgtgcc aacgatataa
    14281 ccaattgcga ttggcgtgac gatgccagaa atgttaccga acatgttgaa caggccaccg
    14341 gaaagaccgc tgatctcttt tggcgcggta tctgccatta ctgcccaacc cagcgccccg
    14401 atgcccttac cgaagaaggc cagcgccata aagccgatga tcatccactc aacgttgacg
    14461 tagttgcaga acaccatcac catcgacagc aacatgccca ttacgatcgg tgttttacgc
    14521 gcaatgttca gcgatcccgt gcggcgcatc agccagtcgg aaataatccc acccagcaca
    14581 ccgccgataa aaccgcaaac cgccggaacg gaagccacaa agcccgcttt cagaattgac
    14641 atcccacgcg cctgcaccag ataaaccgga aaccaggtaa tgaaaaagta agtcagggcg
    14701 ttgatacagt actgaccgat ataaacgccg atcatcatcc gtgacccgag cagttgtttg
    14761 atctgccccc acttcacgct gaacggcact ttaactttgg tgttttgctg atccatattg
    14821 atcagcgcgc cacccgcggc gatgtactcc agctctttct gatttacccc cggatgttga
    14881 tttggctcgt ggatgacttt caaccagata aagctgatga caatccccag accgcccata
    14941 aagaagaaga cgtgtgacca gcccacttca tgcgtcagcc agcccataat cggcgcgaag
    15001 atcaccgttg cgaagtattg agcggagtta aaaatcgaca ccgccgttcc cctttcctgc
    15061 gccggaaacc aagccgcaac aatgcgactg ttgccgggga aagatggcgc ttcagcaagc
    15121 ccgaccagga agcgcagagt aaacagggca acgataatgc cgaatccact aaagatatcg
    15181 acgaagcctt gcagcaaggt aaacatcgac cagataaaga tcgaccagaa gtagacgcgt
    15241 tttgaaccaa aacgatccag caaccaacca ccagggatct gcccgataac ataagcccat
    15301 gagaaggcag agaacacata gcccattccc acgggatcaa ggccgatatc tttggccatt
    15361 tccgaaccgg caatagaaag cgtagcgcgg tcgccgtagt tgaaggatgt gacgataaac
    15421 aacatcacca ctatccagta acgagcattc gtgcgttttt ccacactgct cgcagcctga
    15481 cttaaagaac tcgttgttgc actcctgaaa attcgcgtta gccacgctaa ctctggactg
    15541 cgacatagcc aggaaatcag aggtgacgta gggtgttttt tgccgttttt ataggtcgtt
    15601 cgccgaatac ggcgcgtgtt tatatctggc aatagcagta taaaaagcgc gccatagcgg
    15661 ctcaccgtgc aacaacacaa cattaatgcg ttcaatgagg cccgattttg gcattagccc
    15721 tggacggtgg aatccacttc acggaaatga aaacaagaac aagaaaggaa gggttaaaac
    15781 gaagaaataa agaagagtat gaaatggatc gcttgactcc aggcaaacgc cagtaaaaat
    15841 ccgcgctatg aagcagtttt tactggcatt tgcctgaaaa gattcgattc agcaccgcta
    15901 aaacgacatt taccgctcgc tgaacatatc aggacaacag cgtgccccac tgttcgaccc
    15961 acggatttga ttccgtttcc ggttccgggt tctcactggc gtcaatcaac agcatttcgc
    16021 caactcgctg agcgctctgt tcctgcaaca aggcatcgaa ctgtttgccg ccattgcaga
    16081 aatttacata actgctgtcg ccgagcgcaa tcacgccata acgcagattc ggctggaagc
    16141 ccagactatc tttgattccc tgaaagagcg gcacaatgct atcaggaagg tcgccctgcc
    16201 cggtcgtgga cgtaaccacc agaacatact tatcctgata aggcagccag tcgcttaatt
    16261 caggatcttc aaataccgtt gctttgtggc cctgtgcggt cagaattgct tctgcttctt
    16321 ccgccactaa cagcgaattt ccgtacatgg tgccgacaaa aataccaatt tccgccatac
    16381 cgttttctcc ctggattagg aacttatctc tccatcctga cccgatggcg cactgaactc
    16441 aaccctttca ttttcaggaa gcagaccgcg ccagccaaat tgtgataacg cctgcatcca
    16501 ggtgtcgtcc aggcccgcgt gaatagtcag cggctcgcca gtaaaaggat gcgtcaatga
    16561 caactgactg gcatgtagca ttaaccgctg gaggccaaaa tgctcagcac cgctgcgatt
    16621 ctggcgtaaa tcgccatgtt tgctatcgcc aataatcgga tgacgcaaat gggcaagatg
    16681 tcggcgaagc tgatgtttgc gtccggtttt cggctccagt tccaccaggc cgtagcgcgt
    16741 ggtcgggtaa cgtccggtcg ctaccggcat ttctacggtc gccagaccgc gataatgcgt
    16801 cactgctggc tgcgggcctt tatcttcgcg ggcaaattta tcagcgattt tgtccagttc
    16861 ttccaccagt ggataatcca gcaccgcttc ttccatcaac cagccgcgca caatcgcatg
    16921 gtaacgtttc tggatttggt gctgttcaaa ctgttgtgcc agcagccgtc cggcctcgct
    16981 ggataatccc atcaacaaca caccagaagt gggtcggtcc agacgatgag cagtaaaaac
    17041 atgctgtcct atctggttac gcacggtttg catgaccact actttctcgt cgcgatccag
    17101 ccagctacgg tgaaccagcc agccggaggg tttatttacc gaaaccagcc attcatcctg
    17161 atagagtatt tccagcatta gctcgcatca tccgcaaaaa gagcatccag tttttccaac
    17221 tcagccagaa taagcgcgtg ttgcggatgg tccgtcgcca gcgccatttc ataatagggt
    17281 gcaacagcaa aagcgcccgg taacggctgg ttgttattta acaaatcgtg cattcgcggg
    17341 atcagcaccc actgcaacca ctccagtggt tccatggtgt ccataaagaa cggttgggta
    17401 ctattaaatt gatgcggctg gggttcatcg tttcgccagt gctgatgttc acgcagtaac
    17461 gcttcaagcg cctgcaactg gagacgaacg cggtcatgag tggtcataaa gcaacctcaa
    17521 taagaaaaac tgccgcgaag gatagcagga ataaaaaaag ggagcactgt attcacagcg
    17581 ctcccggttc gtttcgcagc attccagcta cttttgttgc tccctgctca tccttgacaa
    17641 cttttcctct ggccttgcgg ccaatcgttc atcctgaact attgcttcct gctcacacca
    17701 ccccgatgtg atacttcatc ctgaagtgtc cttggccttc ctgaccagcc gaatcatcct
    17761 gaccggttct cattctccat cctggaggtg tcctttaacg cgtcctgcgt catcctcttc
    17821 gcttcatcca gaagcctttc cctgaaacac catcctggtg tgtcctgcag aagtgtcatc
    17881 atcctgatgt tcacttcgtt gtctgactcc ctgtcgacga agatagaatc gtctttttcg
    17941 cgaagtctta caaggcgctt acagacaatg ccttaaggaa aatttcatat gaaagttaag
    18001 aattaacaat caacattttg attttaaatg aaaataacga ttcttatctt tttaagtatt
    18061 cgcttatttc ctggcgatct cctacagagt ctgtaagaga tctctcacaa ccagatgggg
    18121 tatttggatt acagaagagg cttaagttga ttaaggaatt ccgcaagatt tgaggctaaa
    18181 tgcgtccgtt tacgtgttcc cagcgtctct ttgcacactt caccggagag attacacacc
    18241 gagatgacct ctaactcttc ttccagcgtc gcgataaata gcgtgggcgg cagttttagc
    18301 cttttctgag tcaccagatg accaatcaga ttttcctgca cccgacggaa gtcgtcttca
    18361 ctccaggttt gcagcaatgt cagtttgata tcgccaaact gcgcgtgcat atccccggca
    18421 aactgagtcg tataaaaggt atgaattgtg ggttgtatca caatatcaaa agcgcgttca
    18481 accgcgttta cattttgttc cccggtaaac ggctggggtt gccagtatac ggcatcttcg
    18541 gtagttgaaa taatgcacgg tgaaggaaca ccgtacagtt cctcacttaa cggccagctt
    18601 ttatgctctt catgccacgc atcgcagtag cgtgcagtaa aatctttcag ggcttgtgcg
    18661 gtcaaatcgt ccactaattt ctctcttcac ttaaaccaga tacacttgtc gtttagttta
    18721 tctggtttat gacggtgaaa catgtcttct tatgcaaacc atcaggcact tgcgggcctg
    18781 actcttggaa aatcaaccga ttaccgggat acctatgacg ccagcctact gcaaggcgtt
    18841 ccacgcagcc tgaatcgcga cccgctgggt ctgaaagcgg ataacctgcc ttttcacggt
    18901 acggatatct ggacgctgta tgaactttcc tggctgaatg cgaaaggttt gccgcaggtc
    18961 gctgtcggtc atgttgaact tgattacacc agcgtaaatc tgattgagtc gaagagtttt
    19021 aagctctatc tcaacagttt taaccagacg cgttttaata actgggatga ggtgcgccag
    19081 acgctggagc gcgacttaag cacttgcgct cagggtaaga ttagcgtggc gttatatcgt
    19141 cttgatgaac tggaaggcca gccgataggt cattttaatg gcacttgcat tgatgaccag
    19201 gatatcacta tcgataacta tgaattcact actgactatc tggagaatgc cacctgtggt
    19261 gaaaaagtag tggaagagac gcttgtcagc cacctgctga aatcaaactg cctgatcacc
    19321 catcaaccag attggggttc gatccaaatt cagtatcgtg gacgccaaat tgacagagaa
    19381 aaactgctgc gttacctggt ctcattccgt catcacaacg agttccacga acagtgcgtg
    19441 gaacgcatct ttaatgacct gttacgcttc tgccagccag aaaaattgag cgtttacgca
    19501 cgttataccc gtcgtggcgg tctggacatt aacccgtggc gcagtaatag cgattttgtc
    19561 ccatcgacca caagactggt tcggcaataa attttttctc aattttgcgt gctggattca
    19621 cgcagaaggt tgtgaaaggt catcaggcag ggctattgta atcaaaggga atgacgatat
    19681 tcgtcccata aggagttttt cttgattaca catattagcc cgcttggctc catggatatg
    19741 ttgtcgcagc tggaagtgga tatgcttaaa cgcaccgcca gcagcgacct ctatcaactg
    19801 tttcgcaact gttcacttgc cgtactgaac tccggtagtt tgaccgataa cagcaaagaa
    19861 ttgctgtctc gttttgaaaa tttcgatatt aacgtcttgc gccgtgaacg cggcgtaaag
    19921 ctggaactga ttaatccccc ggaagaggct tttgtcgatg ggcgaattat tcgcgctttg
    19981 caggccaact tgttcgcggt cctgcgtgac attctcttcg tttacgggca aatccataac
    20041 accgttcgtt ttcccaacct gaatctcgac aactccgtcc acatcactaa cctggtcttt
    20101 tccatcttgc gtaacgctcg cgcgctgcat gtgggtgaag cgccaaatat ggtggtctgc
    20161 tggggcggtc actcaattaa cgaaaacgag tatttgtatg cccgtcgcgt cggaaaccag
    20221 ctgggcctgc gtgagctgaa tatctgcacc ggctgtggtc cgggagcgat ggaagcgccg
    20281 atgaaaggtg ctgcggtcgg acacgcgcag cagcgttaca aagacagtcg ttttattggt
    20341 atgacagagc cgtcgattat cgccgctgaa ccgcctaacc cgctggtcaa cgaattgatc
    20401 atcatgccag atatcgaaaa acgtctggaa gcgtttgtcc gtatcgctca cggtatcatt
    20461 atcttccctg gcggtgtggg tacggcagaa gagttgctgt atttgctggg aattttaatg
    20521 aacccggcca acaaagatca ggttttacca ttgatcctca ccggcccgaa agagagcgcc
    20581 gactacttcc gcgtactgga cgagtttgtc gtgcatacgc tgggtgaaaa cgcgcgccgc
    20641 cattaccgca tcatcattga tgacgccgct gaagtcgctc gtcagatgaa aaaatcgatg
    20701 ccgctggtga aagaaaatcg ccgcgacaca ggcgatgcct acagctttaa ctggtcaatg
    20761 cgcattgcgc cagatttgca aatgccgttt gagccgtctc acgagaatat ggctaatctg
    20821 aagctttacc cggatcaacc cgttgaagtg ctagctgccg acctgcgccg tgcgttctcc
    20881 ggtattgtgg cgggtaacgt aaaagaagtc ggtattcgcg ccattgaaga gtttggtcct
    20941 tacaaaatca acggcgataa agagattatg cgtcgtatgg acgacctgct acagggtttt
    21001 gttgcccagc atcgtatgaa gttgccaggc tcagcctaca tcccttgcta cgaaatctgc
    21061 acgtaatctc ccctcccccg gtgacgtttg ttgccgggtt ttctcgtttt tggtcactta
    21121 ctcatcaact catttcattt gttatatgaa tgtttcttac caccctcacg cgacaaatat
    21181 catcacagtt aatatgtcat acaatttatg ttgcaacgca aacgtttccc tattttcata
    21241 aaccgtattt tatcgctatg aaaaagaaat tatcgccatg attaactaaa agtattgatt
    21301 ttttcagttc aacctacata tattgcgcgc cctggaagaa gtcagatgtc gtttaatggg
    21361 caaatattgc ccttaaattc tcttttactt ttgatttaca gagtaaagcg ttgggataat
    21421 ctatcttcca agtagattat tgtatttgag atcaagatca ctgatagata cataacttgt
    21481 gtgtatcttt ccgccctcaa attattacgg cggtaaatga ttaagccatc gccgatagac
    21541 agatttcatt tttacggtca ggcaccttcc cgggctgaac tggctaaaag ctgaattatt
    21601 tgcattcctc caggagaaat agatggaaac gactcaaacc agcacgattg cgtcgaaaga
    21661 ctctcgtagt gcctggcgca agacagacac catgtggatg ctgggccttt acggcacggc
    21721 aatcggcgcg ggcgtgctgt tcctgccaat caacgccggt gttggcggta tgatcccgct
    21781 gatcatcatg gctatccttg cgttcccgat gacgtttttt gctcaccgcg gcctgactcg
    21841 cttcgtactg tctggtaaaa acccgggcga agacatcacc gaggttgtag aagaacactt
    21901 tggtattggc gcaggtaaac tgattaccct gctctacttc ttcgctatct acccgatcct
    21961 gctggtttat agcgtggcaa tcaccaatac agttgaaagc ttcatgtctc accagctggg
    22021 tatgacgcca ccgccgcgtg cgattctgtc gctgatcctg atcgtgggta tgatgaccat
    22081 cgttcgcttc ggtgagcaga tgatcgttaa agcgatgagt attctggtgt tcccgtttgt
    22141 tggcgtattg atgctgctgg ctctgtacct gatcccgcag tggaacggcg cagcactgga
    22201 aacgctgtct ctggacactg catctgcaac cggaaacggt ctgtggatga ccctgtggct
    22261 ggcaattccg gtaatggtgt tctcgttcaa ccactctccg atcatctctt ctttcgccgt
    22321 tgcgaagcgt gaagagtacg gcgatatggc agaacagaaa tgctcgaaga tcctggcatt
    22381 cgcacacatc atgatggtgc tgaccgtaat gttcttcgtc ttcagctgtg tactgagcct
    22441 gactccggca gacctggctg cggctaaaga gcagaacatc tcgattctgt cttacctggc
    22501 taaccacttt aacgcaccgg ttatcgcgtg gatggctccg attatcgcga ttatcgctat
    22561 caccaaatcc ttcctcggcc actacctggg cgcacgtgaa ggcttcaacg gtatggtgat
    22621 taaatctctg cgtggtaaag gtaagtctat cgaaatcaac aagctgaacc gtatcactgc
    22681 gctgttcatg ctggtaacga cctggattgt tgccaccctg aacccgagca tcctgggtat
    22741 gattgaaacc ctgggcggtc caatcatcgc gatgatcctg ttcctgatgc cgatgtacgc
    22801 aattcagaaa gtaccggcaa tgcgtaagta cagcggtcac atcagcaacg tattcgttgt
    22861 cgtgatgggt ctgattgcaa tctccgcaat cttctactct ctgttcagct aagtcctttc
    22921 gcgccgcttt cgggcggcgc ttcctccgtt ttaacgcgat gtatttccta tgattagcgt
    22981 attcgatatt ttcaaaatcg gcattggccc ttccagttct cataccgttg gaccaatgaa
    23041 agcgggtaaa caatttaccg acgatctgat tgcccgtaac ctgcttaaag acgtgacccg
    23101 cgtggtggtt gacgtgtacg gctcgctctc tctgaccggt aaaggccacc acactgatat
    23161 cgccattatt atgggcctgg cgggtaacct gccggatacc gtggatatcg attccatccc
    23221 tggttttatt caggatgtga atactcatgg tcgcctgatg ctggcaaacg gtcagcatga
    23281 agtggagttc ccggttgatc agtgcatgaa cttccacgcc gacaaccttt ctctgcatga
    23341 aaacggtatg cgcattaccg cgctggcggg cgataaagtc gtttacagcc agacttacta
    23401 ctctattggc ggtggcttta tcgttgatga agagcatttt ggccagcagg atagcgcacc
    23461 ggttgaagtt ccttatccgt acagttcagc agccgatctg caaaaacatt gtcaggaaac
    23521 cgggctgtca ctctctggcc tgatgatgaa aaacgagcta gcgctgcaca gcaaagaaga
    23581 gctggaacag cacctggcga acgtatggga agtcatgcgt ggcggtattg agcgcggtat
    23641 ttccaccgaa ggcgtgttgc ctggcaaact gcgcgttcca cgccgtgctg cggcactacg
    23701 ccggatgctg gtcagccagg ataaaaccac cactgacccg atggcggttg ttgactggat
    23761 caacatgttt gcactggcag tgaacgaaga gaacgctgct ggcggacgcg tggtgactgc
    23821 gccgactaac ggtgcgtgcg ggattatccc ggcagtgctg gcgtactacg acaagtttat
    23881 ccgcgaagtg aacgctaact cactggctcg ttacctgctg gtagccagtg ccattggttc
    23941 tctttataag atgaacgcgt caatttctgg tgctgaagtc ggctgccagg gtgaagttgg
    24001 tgtggcgtgc tcaatggcgg cggctggtct ggcagaactg ttaggtgcaa gcccggcgca
    24061 ggtgtgcatc gcggcggaaa tcgccatgga gcacaacctc ggtctgacgt gtgacccggt
    24121 cgccggacag gtccaggtgc catgcatcga gcgtaacgcc attgcggcag taaaagcggt
    24181 gaacgccgca cgtatggcgc tacgccgtac cagcgagccg cgcgtctgcc tcgataaagt
    24241 tatcgaaacc atgtacgaaa caggtaaaga tatgaacgcc aagtaccgcg aaacctctcg
    24301 cggcggcctg gcaatgaaga tcgttgcctg cgattaatcg ctctccagag gcctcgtttt
    24361 gcgaggcctc ttcccgattt ctcatccagc cgtagcctgt tccggcatcg aatgttaccc
    24421 ttatcgcctg atctttaagg gggttatcgt ggctgttcat ttgcttattg tcgatgcact
    24481 gaatcttatt cgtcgcattc atgccgttca gggatcgccc tgtgtcgaaa cctgccagca
    24541 tgcgctcgat cagctcatta tgcacagcca gccaacccac gcggtcgccg tttttgatga
    24601 tgaaaaccgc agtagcggct ggcgtcatca gcgtttacca gattacaaag cgggtcgacc
    24661 gccaatgccg gaagagttgc acgacgagat gcctgcatta cgcgccgcct ttgagcaacg
    24721 cggcgtcccg tgctggtcaa ccagcggcaa cgaagccgat gacttagccg ccacgctggc
    24781 ggtcaaagtg acacaggccg ggcatcaggc aacgattgtt tcgacagata aaggctactg
    24841 tcagttactt tcaccgacat tacgtattcg tgattacttc cagaaacgtt ggctggatgc
    24901 gccatttatc gataaagaat ttggcgttca accgcagcag ttgcccgatt actggggact
    24961 tgcggggatc agcagttcaa aggtaccggg tgttgcggga atcggaccaa aaagcgccac
    25021 gcagctgctg gtcgagtttc agagtctgga agggatatat gagaatctgg atgcggttgc
    25081 cgaaaagtgg cgcaaaaaat tagaaaccca taaagagatg gcgtttctgt gccgcgatat
    25141 tgcccgctta caaaccgatt tgcatatcga cggcaattta cagcaattgc ggttggtacg
    25201 gtaacggcga gccggatgca aacgattatc aacacattga atttaatgca cttatatacc
    25261 ggataagacg cggctagcgt cgtatccggc attatcacat cagcgcattt accaggcggt
    25321 atggtaaagc tctacaatat cctcaagcgt tgcttcacgc gggttgccac cggtacaaac
    25381 atcatccagt gccgcctgcg ccagtgccgg aatgtcttcc ttgcgtacac caacatcacg
    25441 caaatgtggc ggaataccga catcacggtt gagagcaaac accgcttcaa cagcggcatt
    25501 acgcgcctct tccagccact ttcacgccca taacgcgcgc gatatcgcgg tacttctcac
    25561 cggtaaagtc agcgttatag cgcatgacat gcggtaacag gatggcgttc gcaacaccgt
    25621 gtggagtgtt gtaaaacgcg cccagtggat gcgccatacc atgcaccaac cctaacccaa
    25681 cattcgagaa gcccataccc gcaacatact gcccgagcgc catttcttct ccggcatcct
    25741 tatcaccagc aaccgatcct cgcagcgccc cagcaatgat ttcaatcgct ttaatgtgca
    25801 gtgcatcggt tagcgcccac gcgccacggg taatataccc ctcaatagca tgagtgagcg
    25861 catcgacacc cgtcgcagct ttcagcgctg gaggcatacc atccatcatg tcagcgtcaa
    25921 taaacgccac ctgcgggata tcatgcggat caacgcaaac aaacttgcgc cgtttctctt
    25981 cgtcagtgat cacgtagtta atggtcactt ctgccgcagt acctgctgtg gtaggaattg
    26041 ccagaatcgg tacactgggt ttattggtcg gggaaagccc ttccaggcta cgcacatcgg
    26101 caaactccgg gttgttgctg ataatgccaa tcgctttaca agtatcctgt ggagaaccac
    26161 caccaatagc gatcaggtaa tccgcgccgc tattctggaa tacaccgagc ccttctttga
    26221 cgacagtaat tgttgggttg ggcactacgc cgtcgtaaat cgcccatgcc agccctgcag
    26281 catccatctt atcggtcact ttcgccacca cgccgcattg caccagcgtt ttatcggtga
    26341 cgatcagcgc cttctgataa ccacggcgtt tcacctcatc ggttaaagcc ccaacagcac
    26401 cccgaccaaa ccatgccgtt tcgttcagaa tcattctgtt agccatcatc cttctccttg
    26461 ttgctttacg aaattactct tcaatccgta acccataggt tttgaatttc tccagcacta
    26521 cggcaatctc ttcatcgctc agcactggca ccgggtccgt aatcgccagg gtcgtcaggt
    26581 aaagttgcgc cagcacttca acttcatgcg ccagccataa cgctttttcc agattcacct
    26641 cacaagcgat aagcccatga tgttgtaaca aagttgcctt acgatttttg agagccagcg
    26701 caacatgttc agaaagttcg cgtgttccaa aggtcgcata agacgcgcaa ggaatagaat
    26761 taccacctgc cgccgcaatc atgtagtgaa tagcggggat cggtcggtta agaatggaaa
    26821 ctgctgtgca atgaacggca tgattgtgaa caaccgcgtt ggcatccggt ctgctttgat
    26881 aggccgccat atggaaacgc cattcgcttg aggggagctt tccttcctca tgtttaccgt
    26941 taccatcaat aaaggcaata tgcgactccg tcagtttttc atatggaatg cctgtaggcg
    27001 taatcagcat cccatcctga taacgtacac tgacgttccc cgctgtcccc tggttcagtc
    27061 ccaggcgggt catttccagg caagtgtcaa taatctgacg agcaagttta tttcgttcca
    27121 ttagctacct ctctctgatt caaaacaggg caataatgtt gttcctttca cactattgaa
    27181 ttagccgttt aattacccac catat
//
LOCUS       DAZPEB010000033        27086 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZPEB010000033 DAZPEB010000000
VERSION     DAZPEB010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27086
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.034"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..334)
                     /locus_tag="ACHURJ_001264"
     CDS             complement(<1..334)
                     /locus_tag="ACHURJ_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR1280285.1"
                     /translation="MNKEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGE"
     gene            complement(415..1842)
                     /locus_tag="ACHURJ_001265"
     CDS             complement(415..1842)
                     /locus_tag="ACHURJ_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFR1280286.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETEPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLDIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKQLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHLLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTEKQTVEPEPQPQPMPVDDGGPGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(1839..2546)
                     /locus_tag="ACHURJ_001266"
     CDS             complement(1839..2546)
                     /locus_tag="ACHURJ_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR1280287.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            2709..3686
                     /gene="ybeQ"
                     /locus_tag="ACHURJ_001267"
     CDS             2709..3686
                     /gene="ybeQ"
                     /locus_tag="ACHURJ_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HFR1280288.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEHAIFWLKKTALQGHTFASN
                     ALGWILDRGEDPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(3756..4238)
                     /locus_tag="ACHURJ_001268"
     CDS             complement(3756..4238)
                     /locus_tag="ACHURJ_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFR1280289.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            4473..7055
                     /gene="leuS"
                     /locus_tag="ACHURJ_001269"
     CDS             4473..7055
                     /gene="leuS"
                     /locus_tag="ACHURJ_001269"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFR1280290.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKS
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            7070..7651
                     /gene="lptE"
                     /locus_tag="ACHURJ_001270"
     CDS             7070..7651
                     /gene="lptE"
                     /locus_tag="ACHURJ_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFR1280291.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            7651..8682
                     /gene="holA"
                     /locus_tag="ACHURJ_001271"
     CDS             7651..8682
                     /gene="holA"
                     /locus_tag="ACHURJ_001271"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFR1280292.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRTLHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            8684..9325
                     /gene="nadD"
                     /locus_tag="ACHURJ_001272"
     CDS             8684..9325
                     /gene="nadD"
                     /locus_tag="ACHURJ_001272"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFR1280293.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIANKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNQED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            9349..9959
                     /locus_tag="ACHURJ_001273"
                     /pseudo
     CDS             9349..9959
                     /locus_tag="ACHURJ_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
     gene            10219..10536
                     /gene="rsfS"
                     /locus_tag="ACHURJ_001274"
     CDS             10219..10536
                     /gene="rsfS"
                     /locus_tag="ACHURJ_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFR1280294.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            10540..11007
                     /gene="rlmH"
                     /locus_tag="ACHURJ_001275"
     CDS             10540..11007
                     /gene="rlmH"
                     /locus_tag="ACHURJ_001275"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFR1280295.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            11038..12939
                     /gene="mrdA"
                     /locus_tag="ACHURJ_001276"
     CDS             11038..12939
                     /gene="mrdA"
                     /locus_tag="ACHURJ_001276"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFR1280296.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTTHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            12942..14054
                     /gene="mrdB"
                     /locus_tag="ACHURJ_001277"
                     /gene_synonym="rodA"
     CDS             12942..14054
                     /gene="mrdB"
                     /locus_tag="ACHURJ_001277"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFR1280297.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            14065..15153
                     /gene="rlpA"
                     /locus_tag="ACHURJ_001278"
     CDS             14065..15153
                     /gene="rlpA"
                     /locus_tag="ACHURJ_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFR1280298.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            15293..16504
                     /gene="dacA"
                     /locus_tag="ACHURJ_001279"
     CDS             15293..16504
                     /gene="dacA"
                     /locus_tag="ACHURJ_001279"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFR1280299.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVIGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDMYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            16615..16878
                     /gene="ybeD"
                     /locus_tag="ACHURJ_001280"
     CDS             16615..16878
                     /gene="ybeD"
                     /locus_tag="ACHURJ_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFR1280300.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVEILYEELGKIDIVRMVL"
     gene            16979..17620
                     /gene="lipB"
                     /locus_tag="ACHURJ_001281"
     CDS             16979..17620
                     /gene="lipB"
                     /locus_tag="ACHURJ_001281"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFR1280301.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            17879..18832
                     /locus_tag="ACHURJ_001282"
     CDS             17879..18832
                     /locus_tag="ACHURJ_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFR1280302.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIALNLLTIFEAVYVHK
                     GIVNAAKVLNLPPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEEQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNKEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            19041..20006
                     /gene="lipA"
                     /locus_tag="ACHURJ_001283"
     CDS             19041..20006
                     /gene="lipA"
                     /locus_tag="ACHURJ_001283"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFR1280303.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVAIEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(20107..20310)
                     /gene="tatE"
                     /locus_tag="ACHURJ_001284"
     CDS             complement(20107..20310)
                     /gene="tatE"
                     /locus_tag="ACHURJ_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFR1280304.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(20439..21227)
                     /locus_tag="ACHURJ_001285"
     CDS             complement(20439..21227)
                     /locus_tag="ACHURJ_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFR1280305.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            21320..21703
                     /gene="crcB"
                     /locus_tag="ACHURJ_001286"
     CDS             21320..21703
                     /gene="crcB"
                     /locus_tag="ACHURJ_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFR1280306.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLTANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(21758..21967)
                     /gene="cspE"
                     /locus_tag="ACHURJ_001287"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(21758..21967)
                     /gene="cspE"
                     /locus_tag="ACHURJ_001287"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFR1280307.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(22142..22702)
                     /gene="pagP"
                     /locus_tag="ACHURJ_001288"
     CDS             complement(22142..22702)
                     /gene="pagP"
                     /locus_tag="ACHURJ_001288"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFR1280308.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAPTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            23291..24676
                     /gene="dcuC"
                     /locus_tag="ACHURJ_001289"
     CDS             23291..24676
                     /gene="dcuC"
                     /locus_tag="ACHURJ_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFR1280309.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(24717..25397)
                     /gene="dpiA"
                     /locus_tag="ACHURJ_001290"
                     /gene_synonym="citB"
     CDS             complement(24717..25397)
                     /gene="dpiA"
                     /locus_tag="ACHURJ_001290"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFR1280310.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTSARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(25366..27023)
                     /gene="dpiB"
                     /locus_tag="ACHURJ_001291"
                     /pseudo
     CDS             complement(25366..27023)
                     /gene="dpiB"
                     /locus_tag="ACHURJ_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
BASE COUNT         6884 a         6800 c         6602 g         6800 t
ORIGIN      
        1 cttccccaca gcacatagcg gtttgggcaa cgaattgggt ataaatcaac cctctctccg
       61 agccaccagc aattaatgct ttccactctt caggggaaga gcgatgccat gtcagatata
      121 agccagctaa atcattggca tgaccatcat cagtcatctg ataaatagtc tgaattaaat
      181 catcacgatt atttattccc caggatgatg agagatcgaa ttcttttata ccggtataca
      241 tttttggcga gtcatgcgcg ggtttgcaac cctgattaag tatttccatg ggcgcagaaa
      301 gcgcaaaaag aagatattgt tcttccttat tcatttgtta ataatccctt aagtgcatta
      361 actgactact tttttatatt acttgccggg ctttccgcac attaacgtcg actgtcaccc
      421 gaacagatgg agtattttcc ctatcaaacc ggcaaagata aaaatgtaga aaataatttt
      481 gatgacgccc agacagcccg gcccaccatc gtcaacgggc attggctgcg gttgcggctc
      541 cggttcaaca gtttgcttct cggtaaaatt cgccgcctga attaataatc gacgaataat
      601 taatgcttcc agtggacaac gatcttctgc atcaagacgg cttacgatcg gcattaatga
      661 ttcttctgtc ggttggctgg cctgcactaa ttgtttggca actaaatata atggccataa
      721 gagatgttct gtatcacgcg tgagtgagat ccgcggctta cgtcctttgt gcaaaaccat
      781 ctcgaccagt gaacgcccca gcgcagagag tttttcatcg ctgagtattt cgtataaatg
      841 aaaacgcgta cgggcgctgt cgccggggac ataaagtagc tgtttaccct gatcgttaac
      901 cagttcacga gcttcaatca tcatcaccac taacgggtaa taatcgaccc gatgctgacg
      961 gcaccattgc aatattaaag catccgcctg ggtggaggga tactcgcgcc agtagtcaca
     1021 aagttctgcc agcaagcttt cgccttctcc acaatagacg cgttgcgcgt attcataata
     1081 acgcgcatct tcattatcag gttcagcttc ccgccacgac cgggcgtaat ccagaagttc
     1141 ggggatatcc aggcgacctg cgctatacca gcggagtaaa tttctgtgca gttttgcatc
     1201 atcggggatg atcactgcgc gatgttgcgc taaatacaat gtcagccatt ctggatgcga
     1261 tgaccaaatc cgagcgaaca tttggtaaaa atcgatggtg tcattttgca cggcaacggg
     1321 cagatgcaga atggtctgga agttaaaaac attccccttc gcaatggcat ataaaaagga
     1381 ttccagttct tcttcgtcta tttccccgac gttttcctcc tgcaaccacc gcaatctttc
     1441 tgctaataac accacgcact cgaaggataa atgggcagtg ttcatggcga ttgtgcacag
     1501 cgaccagcgt aattcatcaa tctcttccat cgagcaataa tttaattgct gaatgaatcg
     1561 ctgccaggcg gagggcagaa aacgttcgct atcagaggca agtaacgcgc gaaaggcgag
     1621 cagaatttca tgttctgcta cctcatattc ttctggttgc caaacagatt tagcaggcga
     1681 ctgcgcaatc cgtagcgcct cctcatacgc ctgacgaagt tgtttaaaac cctgcggttc
     1741 ggtttctgga tgaaaggacg gtaacagcgc cagataagcg cggcggataa tatcgacgtc
     1801 agtcgtttcc tctatatcga ggatcttcca gcaatttttc ataagtcgct catatcctta
     1861 agcgtttccg ggcatgtggg gtagtcagcc agtggttgcc aggacaaggt agggtaatac
     1921 gcatcatcac tggcaaataa ttgataaaac atatcattgc cggaatcgtc gtattcttta
     1981 cgcgctaaga attcgcgtaa taaaggcaga tgatcatctt cgggagactg ccaatattgc
     2041 cgaccaaagg tataaccggc aaagtattgc cgccagttac tataataacg tagagcccgt
     2101 agatgaatac gcgactggat ccatagactt tcttcttcac tcaaccagtt attaagtacg
     2161 cccattcgac ttaaaaaacc catccggaca taatcccatg ctttaattcc tccctcacca
     2221 cagcattcgg ctgtgctggc gacaaattgc gcataagcct gaccttgctc atttaattca
     2281 gcaagatagt cacgccattc acatggagaa taacgaaacc agcggtgata aaaccctgcc
     2341 aaccgagcgg catgaccgtt ctcgatcatt cggtgaattg tgcgcagcag gtcatcacgg
     2401 tcatcaattc cccatgagga agaaaggtca aaaaagcatg tacctaaata cattttgggg
     2461 gatgaatgcg aaggtaaaca gcattcattg cgtatttcca tcggagtgga aagtgcaaac
     2521 aatatttttt gcgaccccat gtccatataa tttatcctta aacattcatt taatgaatgt
     2581 aaactacatt tcgagactca gttatttata cttttctgac gcgacataat acgcataaaa
     2641 taaggcttac gcaaaaatgg aacacataaa atataatatg ttttatctac aaccataagg
     2701 aaatgataat gatttttacg tcaagttgct gcgataattt atcaatagat gagattatcg
     2761 aacgtgctga aaaaggcgat tgcgaggctc aatatattgt tgggttttat tataatcgcg
     2821 atagcgcaat tgactctccg gacgacgaaa aggcctttta ctggctgaag ctggccgccg
     2881 agcaaggtca ttgtgaagca caatattcct taggacaaaa gtatactgag gataaaagcc
     2941 gtcataaaga taatgagcat gccatctttt ggctgaaaaa aactgcccta caaggccata
     3001 ctttcgcttc caacgccctt ggctggatac tggatcgtgg agaagacccc aattataaag
     3061 aagcggttgt ctggtatcag atagccgcgg agagcggaat gtcttatgcg caaaataatc
     3121 ttgggtggat gtacagaaat ggcaacggag tcgcaaaaga ctatgcgctg gcatttttct
     3181 ggtacaaaca agctgcatta caaggccata gtgacgcgca aaacaatctg gccgatcttt
     3241 atgaagacgg aaaaggcgtt gctcaaaaca agacactcgc cgcattctgg tatttgaaaa
     3301 gcgcacagca gggtaatcgg cacgcccagt ttcaaattgc atgggattat aacgctggcg
     3361 aaggggtgga ccaggactat aagcaagcga tgtactggta tctgaaggct gccgctcagg
     3421 aaagcgtcgg cgcttacgtc aacatcggtt atatgtataa acacggacaa ggcgttgaga
     3481 aagattatca ggctgccttt gaatggttta cgaaagccgc tgaatgcaat gacgccactg
     3541 cctggtataa cctggccatt atgtatcact acggagaagg aagacctgtc gatctccgac
     3601 aggctctcga cctgtatcgt aaagttcagt catccggaac cagagatgtc agtcaggaaa
     3661 ttcgtgagac tgaagattta ctgtagagat aaaaacaagg gccggggaat ttcattcggc
     3721 ccatattctc tttattgctg tgcgaaatca gactattacg gctcaaacgg gcgtctctgg
     3781 aactggtcat gaccacattt cgggcatagc gtcagcactt ccggtgtgta gatcgggaga
     3841 tggaagtgac atttctcgca gaccagattt cccagcccga ccacttctcc gctgtgataa
     3901 accccatgat gattgaggtc ctggaaaact tcgcgccatt caagctgcgt tttatcggtg
     3961 atatctgcca gctcctgcca taagctttct ttaatcaccc gcataaagac gctgtcagat
     4021 tcttctttca ggctctcttc atagctcatg gcgaactctt ccaggtcacg tcttacagct
     4081 cgcgtcagct catcgacctc ggttcgcgtt aactcccctg tttttattac gcgctcgcgc
     4141 gcctgttcca ccagtgcgtc gatatcacgt tcgccattgc gcaggcgttc gctcagtgac
     4201 gcgaccagtt cacggtaata ttgagcaacc ttgttcatcg tttcatctcc cgggtcgtta
     4261 actgactata ataatagacg ttctttgttc cttgctttgc taatacgccc acaaactctg
     4321 taaattaatg tatgaataat ccgggcatca tttcaacgtc gagtgcgcga aaggctgttt
     4381 tgacgcggac gtttgggcta tgctatgcgg atctgaaaaa ccacctcaac gctacatttg
     4441 tagccgtatt gaaaacagga ccactggctg ccatgcaaga gcaataccgc ccggaagaga
     4501 tagaatccaa agtacagctt cattgggatg agaagcgcac atttgaagta accgaagacg
     4561 agagcaaaga gaagtattac tgcctgtcta tgcttcccta tccttctggt cgactacaca
     4621 tgggccacgt acgtaactac accatcggtg acgtgatcgc ccgctaccag cgtatgctgg
     4681 gcaaaaacgt cctgcagccg atcggctggg acgcgtttgg tctgcctgcg gaaggcgcgg
     4741 cggtgaaaaa caacaccgct ccggcaccgt ggacgtacga caacatcgcg tatatgaaaa
     4801 accagctcaa aatgctgggc tttggttatg actggagccg cgagctggca acctgtacgc
     4861 cggaatacta ccgttgggaa cagaaattct tcaccgagct gtataaaaaa ggcctggtat
     4921 ataagaagac ttctgcggtc aactggtgcc cgaacgacca gaccgtactg gcgaacgaac
     4981 aagttatcga cggctgctgc tggcgctgcg ataccaaagt tgaacgtaaa gagatcccgc
     5041 agtggtttat caaaatcact gcttacgctg acgagctgct caacgatctg gataaactgg
     5101 atcactggcc agacaccgtt aaaagcatgc agcgtaactg gatcggtcgt tccgaaggcg
     5161 tggagatcac cttcaacgtt aacgactatg acaacacgct gaccgtttac actacccgcc
     5221 cggacacctt tatgggttgt acctacctgg cggtagctgc gggtcatccg ctggcgcaga
     5281 aagcggcgga aaataatcct gaactggcgg cctttattga cgaatgccgt aacaccaaag
     5341 ttgccgaagc tgaaatggcg acgatggaga aaaaaggcgt cgatactggc tttaaagctg
     5401 ttcacccatt aacgggcgaa gaaattcccg tttgggcagc aaacttcgta ttgatggagt
     5461 acggcacggg cgcagttatg gcggttccgg gtcacgacca gcgcgactac gagtttgcct
     5521 ctaaatacgg cctgaacatc aaaccagtta tcctggctgc tgacggctct gagccagatc
     5581 tttctcagca agcactgact gaaaaaggcg tgctgttcaa ctctggcgaa ttcaacggtc
     5641 ttgaccatga agcggccttc aacgccatcg ccgataaact gactgcgatg ggcgttggcg
     5701 agcgtaaagt gaactaccgc ctgcgcgact ggggtgtttc tcgtcagcgt tactggggcg
     5761 cgccgattcc gatggtgacg ctggaagacg gtaccgtaat gccgaccccg gacgaccagc
     5821 tgccggtgat cctgccggaa gatgtggtga tggacggcat taccagcccg attaaagcag
     5881 atccggagtg ggcaaaaact accgttaacg gtatgccagc gctgcgtgaa accgacactt
     5941 tcgacacctt tatggagtcc tcctggtact atgcgcgcta cacttgccca gagtacaaag
     6001 aaggtatgct ggattccgaa gcggctaact actggctgcc ggtggatatc tacattggtg
     6061 gtatcgaaca cgccattatg cacctgctct acttccgctt cttccacaaa ctgatgcgtg
     6121 atgcaggcat ggtgaactct gacgaaccag cgaaacagtt gctgtgtcag ggtatggtgc
     6181 tggcagatgc cttctactat gttggcgaaa acggcgaacg taactgggtt tccccggttg
     6241 atgctatcgt tgaacgtgac gagaaaggcc gtatcgtgaa agcgaaagat gcggcaggcc
     6301 atgaactggt ttataccggc atgagcaaaa tgtccaagtc gaagaacaac ggtatcgacc
     6361 cacaggtgat ggttgaacgt tacggcgcgg acaccgttcg tctgtttatg atgtttgctt
     6421 ctccggctga tatgactcta gaatggcagg aatccggtgt ggaaggggct aaccgcttcc
     6481 tgaaacgtgt ctggaaactg gtttacgagc acacagcaaa aggtgatgtt gcggcactga
     6541 acgttgatgc gctgactgaa gatcagaaag cgctgcgtcg cgatgtgcat aaaacgatcg
     6601 ctaaagtgac cgatgatatc ggccgtcgtc agaccttcaa caccgcaatt gcggcgatta
     6661 tggagctgat gaacaaactg gcgaaagcac caaccgatgg cgagcaggat cgcgctctga
     6721 tgcaggaagc gctgctggcc gttgtccgta tgcttaaccc gttcaccccg cacatctgct
     6781 tcacgctgtg gcaggaactg aaaggcgaag gcgatatcga caacgcgccg tggccggttg
     6841 ctgacgaaaa agcgatggtg gaagactcca cgctggtcgt ggtgcaggtt aacggtaaag
     6901 tccgtgccaa aatcaccgtt ccggtggacg caacggaaga acaggttcgc gaacgtgctg
     6961 gccaagaaca tctggtagca aaatatcttg atggcgttac cgtgcgtaaa gtgatttacg
     7021 taccaggtaa actcctcaat ctggtcgttg gctaagcgcg ggaggaagcg tgcgatatct
     7081 ggcaacattg ttgttatctc tggcggtgtt aatcaccgcc gggtgtggct ggcatctgcg
     7141 tgataccacg caggttcctt ccactatgaa ggtcatgatc ctggactcag gcgatccgaa
     7201 cgggccatta agccgtgcgg tgcgtaacca gttacgtctg aatggtgtcg agttgcttga
     7261 taaagaaacc acgcgtaagg acgttccatc cctgcgtttg ggtaaagtga gcatcgcgaa
     7321 agataccgca tcggtattcc gtaacggtca aacagcagag tatcagatga tcatgacggt
     7381 gaatgcgacc gtgttgatcc ccggccgtga tatctacccg attagcgcca aagtcttccg
     7441 ttcgttcttt gataacccgc aaatggcgtt agcgaaagat aacgaacaag acatgatcgt
     7501 aaaagagatg tacgaccgtg ctgccgaaca gctgattcgt aagctgccaa gcatccgtgc
     7561 tgcggatatt cgttccgacg aagaacagac gtcgaccaca acggatactc cggcaacgcc
     7621 tgcacgcgtc tccaccacgc tgggtaactg atgattcggt tgtacccgga acaactccgc
     7681 gcgcagctca atgaagggct gcgcgcggcg tatcttttac ttggtaacga tcctctgtta
     7741 ttgcaggaaa gccaggacgc tgttcgtcag gtagctgcgg cacaaggatt cgaagaacac
     7801 cacacttttt ccattgatcc caacactgac tggaatgcga tcttttcgtt atgccaggct
     7861 atgagtctgt ttgccagtcg acaaacgcta ttgctgttgt taccagaaaa cggaccgaat
     7921 gcggcgatca atgaacaact tctcacactc accggacttc tgcatgacga cctgctgttg
     7981 atcgtccgcg gtaataaatt aagcaaagcg caagaaaatg ccgcctggtt tactgcgctt
     8041 gcgaatcgca gcgtgcaggt gacctgtcag acaccggagc aggctcagct tccccgctgg
     8101 gttgctgcgc gcgcaaaaca gctcaactta gaactggatg acgcggcaaa tcaggtgctc
     8161 tgctactgtt atgaaggtaa cctgctggcg ctggctcagg cactggagcg tttatcgctg
     8221 ctctggccag acggcaaatt gacattaccg cgcgttgaac aggcggtgaa tgatgccgcg
     8281 catttcaccc cttttcattg ggttgatgct ttgttgatgg gaaaaagtaa gcgcacattg
     8341 catattcttc agcaactgcg tctggaaggc agcgaaccgg ttattttgtt gcgcacatta
     8401 caacgtgaac tgttgttact ggttaacctg aaacgccagt ctgcccatac gccactgcgt
     8461 gcgttgtttg ataagcatcg ggtatggcag aaccgccggg gcatgatggg cgaggcgtta
     8521 aatcgcttaa gtcagacgca gttacgtcag gccgtgcaac tcctgacacg aacggaactc
     8581 accctcaaac aagattacgg tcagtcagtg tgggcagagc tggaagggtt atctcttctg
     8641 ttgtgccata aacccctggc ggacgtattt atcgacggtt gatatgaaat ctttacaggc
     8701 tctgtttggc ggcacctttg atccggtgca ctatggtcat ctaaaacccg tggaaacgct
     8761 ggcgaatttg attggtctga cgcgggtcac aatcatccct aataatgttc ctccgcatcg
     8821 tccccagccg gaagcgaaca gcgtgcagcg taaacacatg cttgaactgg cgattgccaa
     8881 caagccatta tttactcttg atgaacgcga gctaaagcgc aatgccccct cttacactgc
     8941 gcaaacactg aaagagtggc ggcaggaaca aggaccggac gtgccgctgg cgtttattat
     9001 tggtcaggat tcactgctga cctttccgac ctggtacgaa tacgaaacga tactcgacaa
     9061 tgcacatttg atcgtctgtc ggcgtccagg ttacccactt gaaatggcgc aaccgcaata
     9121 ccagcaatgg ctggaagatc atttgacaca taaccaggaa gatcttcacc ttcagcctgc
     9181 cggtaaaatt tatctggctg aaacgccgtg gtttaacatc tcggcgacca tcatccgcga
     9241 acgtttgcaa aacggtgaat cgtgtgagga tttattgccg gaaccggtac tgacttacat
     9301 taaccaacaa ggcttgtatc gctgatacct gtcgttacgg agggcatgat gcgactgtgg
     9361 ttaattcgtc atggtgaaac gcaagcgaat atcgatggtc tttacagcgg tcatgcgccc
     9421 cccccctgac cgcgcgcggt attgagcaag cgcaaaatct gcatacgctg ctacatggtg
     9481 tttcctttga tctggtttta tgcagtgaac tggaacgggc acagcatacc gcgcgactgg
     9541 ttctcagtga ccgccagctc cccgtgcaaa tcatccctga actcaacgaa atgttttttg
     9601 gcgactggga gatgcgacat catcgcgacc tcatgcaaga agatgccgaa aactatagcg
     9661 cgtggtgcaa tgactggcag catgcaatcc ccacgaacgg tgaaggattt caggcatttt
     9721 cgcaacgtgt ggaacgcttt atcgcaaggc ttagtgaatt tcagcactat cagaatattt
     9781 tagtcgtcag ccatcagggt gtactgagtc tgttaatcgc ccgtttaatt ggcatgcctg
     9841 ccgaggctat gtggcatttt cgcgttgacc aggggtgttg gagcgccatt gatatcaacc
     9901 aaaaattcgc gacgctacgc gtcctcaata gccgtgccat cggggtcgat aatgcatgac
     9961 ttttctgttt ttttacgggt aagccgcaac gaccattgac agacccgggc aggctgatat
    10021 tctccgcagc cagacttttt tcgccagaca cgactttgta gaaattgttt tacaaaaatg
    10081 gcgatgcaat ctgcggcgcg gggtgggatg atagcccact ttcgaaagcc gattcggcga
    10141 caattgtccc gaaatcgcct ctggttcagg tatactgaca gaccattttt atctatttga
    10201 ttcacccagg gggaaaactt gcagggtaaa gcactccagg attttgttat cgacaaaatt
    10261 gatgacctca aaggtcagga catcatcgcc ttagacgttc aaggcaaatc cagcatcacc
    10321 gactgcatga tcatctgtac gggtacgtcc agccgtcatg ttatgtccat tgctgaccac
    10381 gttgtgcagg agtctcgcgc agcgggcctg ttaccgctcg gcgtagaagg tgaaaacagc
    10441 gccgactgga ttgtcgtgga tttgggcgat gtgattgtcc atgtcatgca ggaagagagc
    10501 cgtcgcctgt atgaactgga aaaactctgg agttaatgcg tgaagctgca acttgtcgcc
    10561 gtgggaacga aaatgccgga ctgggtacaa accggtttta ccgagtacct gcgtcgtttt
    10621 ccgaaagata tgcccttcga gctgattgaa attccggccg gaaaacgcgg caagaatgcg
    10681 gacatcaagc gcatactcga caaagagggt gagcagatgt tggcggccgc aggcaaaaac
    10741 cgcattgtca ccctcgatat tccaggcaag ccctgggata cgccgcagtt agccgctgag
    10801 ctggaacgct ggaagctgga tggtcgcgac gtcagtctac tgattggcgg gcctgaaggg
    10861 ttgtcgcctg cctgtaaagc ggcggcggag cagagctggt cgctgtcggc gcttaccctc
    10921 ccccatccgc tggttcgcgt gctggtcgca gagagtctgt accgggcgtg gagcatcacc
    10981 accaaccatc cttatcaccg tgagtgataa gggagctttg agtagaaaac gcagcggatg
    11041 aaactacaga actcttttcg cgactatacg gcagagtccg cgctgtttgt gcgccgggcg
    11101 ctggtcgcct ttttggggat tttgctgctg accggcgtgc ttatcgccaa cctgtataat
    11161 ctgcaaattg ttcgctttac cgactaccag acccgctcta atgaaaaccg cattaagctg
    11221 gtgcctatcg cgcccagccg cggcattatt tacgaccgta atggtatccc tctggccctc
    11281 aaccgcacta tctaccagat agaaatgatg ccagagaaag tcgataacgt gcagcaaacg
    11341 ctggacgctt tgcgcagcgt ggttgatctg accgatgacg atattgctgc attccgaaaa
    11401 gagcgcgcac gttcacaccg tttcacctct attcccgtga aaactaacct gaccgaagta
    11461 caagtagctc gctttgccgt caatcagtac cgttttccgg gggttgaagt taaaggctat
    11521 aaacgtcgtt actatcctta cggttcggcg ttgacccacg tcatcggcta tgtgtcgaaa
    11581 atcaacgata aagacgtcga acgcctgaat aatgacggca aactggccaa ctatgcggca
    11641 acgcatgata tcggtaagct gggcattgag cgttactatg aagacgtgct gcacggtcag
    11701 accggttatg aagaggttga agttaacaac cgtgggcgtg ttatccgcca gttaaaagaa
    11761 gtaccaccgc aagccggaca cgatatttac ctgacgctgg atctcaaact ccagcaatat
    11821 attgaaacgc tgctggcggg tagccgcgca gctgtggtag tcaccgatcc gcgtacaggt
    11881 ggggtgctgg cgctggtttc cacgcctagt tatgacccaa acttgtttgt tgacggtatc
    11941 tccagcaaag attattccgc cttgttgaac gacccgaata caccgctggt gaaccgcgcc
    12001 acacaggggg tttatcctcc cgcgtctaca gttaaaccct atgtggcggt ttcggcattg
    12061 agcgccgggg tgatcacgcg caatacgacg ctgtttgacc caggctggtg gcaactgcca
    12121 ggttcggaaa aacgttatcg tgactggaaa aaatggggcc acgggcgtct gaatgtcaca
    12181 agatcgctgg aagaatctgc ggataccttc ttctatcagg tggcctacga tatggggatc
    12241 gatcgcctct ccgaatggat gggtaaattc ggttatggtc attacaccgg tatcgacctg
    12301 gcggaagaac gttccggcaa catgcctacc cgcgaatgga aacagaaacg ctttaaaaaa
    12361 ccgtggtatc agggtgacac cattccggtt ggtatcggtc agggttactg gacagcgacc
    12421 ccaatccaga tgagtaaggc actgatgatc ctgattaatg acggtatcgt gaaggttccg
    12481 catttgctga tgagcaccgc cgaagacggc aaacaggtgc catgggtaca accgcatgaa
    12541 ccgcccgtcg gcgatattca ttccggttac tgggagctgg cgaaagacgg tatgtacggt
    12601 gttgctaacc gccctaacgg tacgacgcat aaatactttg ccagcgcacc gtacaaaatt
    12661 gcggcgaaat ctggtaccgc tcaggtcttc ggtctgaaag cgaacgaaac ctataatgcg
    12721 cacaaaattg ccgagcgttt acgtgaccac aaactgatga ccgcctttgc gccatacaac
    12781 aatccgcaag tggctgtcgc catgattctg gagaacggcg gcgcgggtcc ggcggttggt
    12841 acgctgatgc gtcagatcct cgaccacatt atgctgggtg ataacaacac cgatctgcct
    12901 gcggaaaatc cagcggttgc cgcagcggag gaccattaat catgacggat aatccgaata
    12961 aaaaaacatt ctgggataaa gtccatctcg atcccacaat gctgctgatc ttactggcat
    13021 tgctggttta cagcgcccta gttatctgga gcgccagcgg tcaggatatt ggcatgatgg
    13081 agcgtaaaat cggccaaatc gcgatgggtc tggtcatcat ggtggtgatg gcgcaaattc
    13141 ctccacgcgt ttatgaaggc tgggccccct atctctatat catctgtatt attttgctgg
    13201 tggcggtaga tgctttcggt gccatctcta aaggtgctca acgctggctg gacctcggta
    13261 ttgttcgttt tcagccgtcg gaaattgcca aaatagccgt accactgatg gttgcgcgct
    13321 ttatcaaccg cgacgtttgc ccaccgtcgt tgaagaacac aggcatcgcg ctggtgctga
    13381 tatttatgcc cacgctgctg gtggctgcac agcctgacct gggaacatca atcctcgttg
    13441 cgctttccgg tctgtttgta ctgttcctct ctggccttag ctggcgtctg attggcgtcg
    13501 cagtagtgct ggtggcggcg ttcattccta ttctgtggtt cttcctgatg catgattacc
    13561 agcgccagcg cgtaatgatg ctcctagacc cggaatcaga cccactcggc gcgggctatc
    13621 acattattca gtctaaaatt gctattggct ccggcggatt acgcggcaaa ggctggctgc
    13681 atggcactca gtcacaactt gaatttctac ccgaacgcca taccgacttt atcttcgcgg
    13741 tactggcgga agagctggga ttagtgggca ttttgattct gctcgctctc tacattctgc
    13801 tgatcatgcg cgggctgtgg atagccgcca gagcgcaaac cacctttggt cgcgtcatgg
    13861 ctggcggctt aatgctgata ttattcgttt atgtcttcgt aaatattggt atggtaagcg
    13921 gtattctgcc ggttgtaggg gttccgctcc cactggtcag ttatggagga tcagcgctaa
    13981 ttgtgctgat ggctgggttc gggattgtaa tgtcaatcca cacccacagg aaaatgttgt
    14041 cgaaaagcgt gtaagaggtg cgcaatgcgt aagcagtggc tcgggatctg catcgcggca
    14101 ggaatgctcg cggcatgtac aagcgatgat ggtcagcaac agacggtaag tgtaccgcag
    14161 cctgcggtat gtaacggccc catagttgaa attagcgggg cggacccgcg tttcgaacca
    14221 ctgaacgcga cggcaaatca ggattaccag cgcgacggta aaagctacaa aatcgtgcag
    14281 gatccgtctc gatttagcca ggcgggactg gcggcaatct atgatgccga accaggcagt
    14341 aacctgacgg cctctggcga agctttcgat ccgacacagc tgacggcggc ccatccaacg
    14401 cttccgatcc ccagctacgc cagaatcact aacctggcta acgggcgaat gatcgtggtg
    14461 cgcattaatg atcgcggccc ttacggcaac gaccgcgtta tttcgctttc tcgcgcggca
    14521 gctgaccgtc ttaacacgtc aaacaacacc aaagttcgta tcgatccgat tattgtcgcc
    14581 caggatggtt cgctttctgg tcctggtatg gcttgtacca cagtcgccaa acagacttac
    14641 gccctgcctg cacctcccga tttaagcggt ggcgcgggaa caagttcagt gtctggcccg
    14701 cagggtgaca ttcttccggt cagtaattcg acgctaaaaa gcgaagatcc gaccggcgcg
    14761 ccggtaacca gtagcggttt cctcggcgca ccaacgacct tagcgcctgg tgtactggaa
    14821 ggcagcgaac cgacgcctgc gccacagccc gttgttacag ctccgtcgac aacgcctgca
    14881 acctcgcctg caatggtgac accgcaagcc gcctcgcaaa gcgccagcgg caactttatg
    14941 gtgcaagtcg gggccgttag cgatcaggct cgtgcgcaac agtaccaaca gcaactggga
    15001 cagaagttcg gcgtccccgg tcgcgtaact caaaatggcg cggtctggcg gatccagctt
    15061 ggccccttcg ccagcaaagc cgaagccagt accttgcagc aacgtttgca aaccgaagcc
    15121 caattacagt catttattac caccgcgcag tagcgtaaag caggcatctg atgtgtcaat
    15181 ttctgtaaat gacgttaaca aagtcacgcg gaaagtcaga tgcctgccgg tagtgcattt
    15241 gctatagtag ggcacttttt ttaattccat cacggatgtc gtagttcaga ccatgaatac
    15301 cattttttcc gctcgtatca tgaagcgcct ggcgctcacc acggctcttt gcacagcctt
    15361 tatctctgct gcacatgccg atgacctgaa tatcaaaact atgatcccgg gtgtaccgca
    15421 gatcgatgcg gagtcctaca tcctgattga ctataactcc ggcaaagtgc tcgccgaaca
    15481 gaacgcagat gtccgccgcg atcctgccag cctgaccaaa atgatgacca gttacgttat
    15541 cggccaagca atgaaagccg gtaaatttaa agaaactgat ttagtcacta tcggcaacga
    15601 cgcatgggcc accggtaacc cggtgtttaa aggttcttcg ctgatgttcc tcaaaccggg
    15661 catgcaggtt ccggtttctc agctgatccg cggtattaac ctgcaatcgg gtaacgatgc
    15721 ttgtgtcgcc atggctgatt ttgccgctgg tagccaggac gcttttgttg gcttgatgaa
    15781 cagctacgtt aacgccctgg gcctgaaaaa cacccacttc cagacggtac atggtctgga
    15841 tgctgatggt cagtacagct ccgcgcgcga tatggcgctg atcggccagg cgttgatccg
    15901 tgacgtaccg aatgaatact cgatctataa agaaaaagaa tttacgttta acggtattcg
    15961 ccagctgaac cgtaacggcc tgttatggga taacagcctg aatgtcgacg gcatcaaaac
    16021 cggacacact gacaaagcag gttacaacct tgttgcttct gcgactgaag gccagatgcg
    16081 cttgatctct gcggtgatag gcggacgtac ttttaaaggc cgtgaagccg aaagtaaaaa
    16141 actgctgacc tggggcttcc gtttcttcga aaccgttaac ccactgaaag taggtaaaga
    16201 gttcgcctct gaaccggttt ggtttggtga ttctgatcgc gcttcgttag gggttgataa
    16261 agacatgtac ctgaccattc cgcgtggtcg catgaaagat ctgaaagcca gctatgtgct
    16321 gaacagcagt gaattgcatg cgccgctgca aaagaatcag gtcgtcggaa ctatcaactt
    16381 ccagcttgat ggcaaaacga tcgagcaacg cccgctggtt gtgttgcaag aaatcccgga
    16441 aggtaacttc ttcggcaaaa tcattgatta cattaaatta atgttccatc actggtttgg
    16501 ttaaaaatta aacacttgaa agtgtaattt ccgtccccat atactaagca tcagtaaaaa
    16561 aactcccgcc ttctggcggg agttgctatt taattacgtt acgccggagc tgacatgaaa
    16621 accaaactta acgaactgct tgaattccct actcctttta cttacaaagt tatggggcag
    16681 gcgttacctg agctggttga tcaggtggtt gaagtggtac agcgccatgc gccaggtgac
    16741 tacaccccaa cggtaaaacc aagcagcaaa ggcaactacc actcggtatc tatcactatc
    16801 aacgccactc atatcgagca ggttgaaata ctgtatgaag aactgggcaa aatcgatatt
    16861 gtccgcatgg ttctgtaact cgcttctccg ttacccggct cctggtcggg taactcccct
    16921 ctcctcgctg tgatatactt ttcccccact tttactcatt ctccacggag atgccgtttt
    16981 gtatcaggat aaaattcttg tccgccagct cggtcttcag ccttacgagc caatctccca
    17041 ggctatgcat gaattcaccg atacccgcga tgatagtacc cttgatgaaa tctggctggt
    17101 cgagcactat ccggtattca cccaaggtca ggcaggaaaa gcggagcaca ttttaatgcc
    17161 gggtgatatt ccggtgatcc agagcgatcg cggtgggcag gtgacttatc acgggccggg
    17221 acaacaggtg atgtatgtgt tgcttaacct gaaacgccgt aaactcggtg tgcgtgaact
    17281 ggtgaccttg cttgagcaaa cagtggtgaa taccctggct gaactgggta tagaagcgca
    17341 tcctcgggct gacgcgcctg gtgtctatgt cggggaaaag aaaatttgct cactgggttt
    17401 acgaattcga cgcggttgtt cattccacgg tctggcatta aacgtcaata tggatctttc
    17461 accattttta cgtattaatc cttgtgggta tgccggaatg gaaatggcta aaatatcaca
    17521 atggaaaccc gaagcgacga ctaataatat tgctccacgt ttactggaaa atattttagc
    17581 gctactaaac aatccggact tcgaatatat taccgcttaa ttccatacat caatggccca
    17641 atttacactg ggtcattact cataatacct gctaatgctt tacatctcct tcgcgtttgt
    17701 atattttctt tatcacttgc agaaatgcgg tgccacgcct tgctccgtaa ctcaccccat
    17761 tgattaacct attgaaatag ttgattatgg tgagcaaaag cgttccgttc caattagaca
    17821 taagattatt gtaatttttt aaatattatt tttccatgaa taaaattgga gtaagtgcgt
    17881 ggatagtaat aatcaaattg aaccctgttt aagcagaaag tcgtcagaag gtaaaccaca
    17941 aatatttaca accttacgaa atattgctct taaccttctg actatttttg aagctgtata
    18001 tgtacataaa gggatcgtta atgcagcgaa agtgcttaat ctgcccccct cggcaatcag
    18061 tcagtctatt cagaaactgc gcgttatatt ccctgaccca ttgtttattc gcaaaggcca
    18121 aggtgtcact cctaccgcat ttgcgatgca tctacatgag tatatcagtc agggccttga
    18181 gtccattctt ggcgcgctgg atatcgaagg aagctatgat aagcaacgaa cgataactat
    18241 tgcgaccact ccctcggtcg gagccctggt ccttcctgtc atctatcggg cgattaaaac
    18301 tcactatccg cagcttttac tgcgcaaccc gcccatcagc gacgcggaaa accaactcag
    18361 tcagtttcaa accgatctca tcatcgataa catgttttgc accaatcgta cggtgcaaca
    18421 tcatgttctg ttcaccgaca atatggtgtt aatttgccgc gagggaaatc cactactctc
    18481 tttagaagat gacagagaga ctatcgacaa cgctgcgcat gtacttctgt taccggaaga
    18541 gcaaaatttc agcggtctgc ggcagagagt tcaagaaatg tttccggacc ggcaaatcaa
    18601 tttcaccagc tacaacattt tgacaatcgc tgcactggtt gccaacagtg acatgttagc
    18661 gattattccc agccgttttt ataacctgtt tagccgctgc tggccgctgg aaaaactgcc
    18721 ttttccgtcc ttaaataagg agcaaataga tttctctatc cactacaaca aattcagcct
    18781 gcgtgatccc atcctgcacg gagtaataga tgttatccgt aatgcatttt gaaaaagtat
    18841 tgttacagcc agatcacagc gcagaacgat aaaatggcac aatccaacaa caattttaca
    18901 ttttcgcgac cgctttggct gctttcaggt ccgtttcaat gatatactgc cagtcgttaa
    18961 ttcaaaaata gttgataatt acaacaatct attgaattga aacgctttcc ttcgtaattc
    19021 gcaactggaa cacgcacgct atgagtaaac ccattgtgat ggaacgcggt gttaaatacc
    19081 gcgatgccga taagatggcc cttatcccgg ttaaaaacgt ggcaatagag cgcgaagccc
    19141 tgctgcgcaa gccggaatgg atgaaaatca agcttccagc ggactctaca cgtatccagg
    19201 gcatcaaagc cgcaatgcgc aaaaatggcc tgcattctgt ctgcgaggaa gcctcctgcc
    19261 ctaacctggc ggaatgcttc aaccacggca cagcaacgtt tatgatcctc ggcgctattt
    19321 gtacccgccg ttgtccgttc tgtgatgttg cccacggtcg cccggtagct cctgatgcca
    19381 atgaaccagt gaaactggcg cagaccattg ccgatatggc gctgcgttat gtggttatca
    19441 cctccgttga ccgtgatgac ctgcgcgatg gcggtgccca gcactttgcg gattgcatta
    19501 ctgccattcg ggaaaaaagc ccgcaaatca aaattgaaac tctggtgccg gatttccgtg
    19561 gtcgtatgga tcgtgctctg gatattctga ctgcaacgcc accagatgtg ttcaaccata
    19621 acctggaaaa cgtaccgcgt atttaccgtc aggtacggcc tggggcagat tacaactggt
    19681 cgctgaagct gctggaacgc tttaaagaag cgcatccgga aatcccgacc aagtctggtc
    19741 taatggtggg actgggtgaa accaatgaag aaattattga ggtaatgcgc gacctgcgcc
    19801 gtcatggtgt gacgatgtta acgctggggc aatatttgca gccaagccgc catcacctgc
    19861 cggttcaacg ttacgttagc ccggatgagt tcgacgaaat gaaagccgaa gcgctggcga
    19921 tgggctttac ccatgctgca tgcggtccgt ttgtccgttc ttcttaccac gccgatttgc
    19981 aggcgaaagg gatggaagtt aagtaatcct gtaataatta cttacatctg tctgataaaa
    20041 aacccgcttt ttgaagcggt ttttttgtat cgaacaagat attgagggag cgtcctgctc
    20101 gccacgtcac tctttatgag agagcttttc agcctgaaga tcaacgtctg cgcctttttt
    20161 cgccgcagca tcatcatcat tcatcgcctt cttgaaccct ttaatggccg ctccaaggtc
    20221 tccgcccagc gtacgtaact tcttagtccc aaacagcaga acgaccagcg ccgcaactac
    20281 tagcagtttg gtaatactaa tctcacccat agataccttc ttgacatata accggcaaca
    20341 gaacgcgcct taaaaaccag agaatattaa cggtcattcg acgcgcgcac acaatagcaa
    20401 tctgtaacaa ggtgaatcaa gcgccgagta aaaaaacatc ataataattg cggcggcgca
    20461 aagcgacggt tgtttaagac gggcagttgc gcgcgcacct gacgcacacg ttcgggcgtc
    20521 acttccgcca taatgagtgc aggcatttct gacgctgccg caatggtgac gccaaaggga
    20581 tctataatcc ggctttgccc gatatttttg ttcccgcact cgcccgccgc caccatataa
    20641 caggtggtat ccagcgcacg agcggcaagc aacgttgacc agtgatgctc tttgagcggc
    20701 ccgcgaaccc aggcggcagg aagtaccagg atttcagctc cctgtaatgc ctgtgccagc
    20761 gccagctctg gaaagcgtaa gtcataacag gtcatcagac cgaccttcat cccttccacc
    20821 tccagtaacg gagcgatttc attaccagca tcaacacggc gtgattcctg aatggcaaat
    20881 gcatcataga gatgcagttt ggcataacgg gcgacgatgt ttcctgcctg aagtgccacc
    20941 agcatattcc atgcgcgccc cggcgttgaa ggaacatgaa tcgtcagaat tgtcgtcatc
    21001 atgttacgtt tactttctcg ccgtaaacgt ccgaggaatt cgccttccag cagctgtgct
    21061 gatttaaccg atagatctgc atcatgatca tcgcgtgcca gcaatgcttc cggcaggaca
    21121 aacagcgata cgtcgttttc cgccgcctgc gccatcaacg aggcacaaat ctcagcgttc
    21181 ttttcccaca cagatgtaac agcaaactgt cctgcagcaa ccagcattgc cacctcctgt
    21241 cagagtcgta aaattcgcca acgacgatta ttcggcgtta cacttatcac tcatacaaat
    21301 caaatagcag gattttgcag tgttacaact tcttttagca gtttttattg gcggtggtac
    21361 gggaagcgtg gcgagatggc tgttaagtat gcgatttaac ccactgcatc aggcgattcc
    21421 gttggggacg ctgacagcaa acctgattgg ggcattcatc ataggaatgg gattcgcatg
    21481 gttcagcagg atgacgaaca ttgatccagt gtggaaagta ttaatcacca ccggattttg
    21541 tggcggtcta acaaccttct caacattttc ggcagaagtg gtgtttttgt tacaagaggg
    21601 tcgctttggc tgggcattac tgaacgtttt cgtcaacctt ctggggtctt ttgccatgac
    21661 agcactggct ttctggctgt tttcggcctc aaccgcacac taaagaccca aaaaaaaccc
    21721 gctgattaag cgggttttga attcttgctg acgtatctta cagagcgatt acgtttgcag
    21781 cagaagggcc tttggcaccg ttagtgattt cgaactctac gcgctgacct tcagcaagag
    21841 ttttaaaacc attagtctgg attgcagaga agtgtacgaa cacgtctttg ctgccgtctt
    21901 ccggagtaat gaaaccgaat cctttggact cattaaacca cttaacgtta cctttaatct
    21961 tagacatcaa aattaccttt acatgaaaaa tagacacaaa tgctgcgtcg gttaccagta
    22021 caccaattgt ggtacgcttt gtccagtgca actttactaa aaagtgataa aagtcgcgtt
    22081 atttttgatg ttacttattt tagctattga ttttaaagaa gttactaaaa cttcatttgt
    22141 ctcaaaactg aaagcgcatc caggcaaagt acacattgcc attgttgtag gtacccggaa
    22201 tgtaggtcat ctgaaaagtc actgggccat aacccacgga ggccaatggc agtagaaccg
    22261 ggagagggat gtaattccag ttatcgcgtg ccgttacgcc agcggtgaat cccagaccta
    22321 aatgaaaatt ttcatccgcc agcggtcgcc aggtactttc ccatccgtat ccggcaatcg
    22381 gttcccattt gttccacgag tccttaaatg ccatggcata caggccatgc cagtttcctt
    22441 tttcatccca acgcgacagg ccaaaaccgc caccccacgg tcgctcgtta tagcgatcgg
    22501 ttttttcttt gtcgtaagcg aaacgtgcat gccaggtgat ggcaggaata tataaatcat
    22561 aatgttcagg ctgttgccag gttggtgcaa tattttctct aaacgttgtc atccactcat
    22621 ctgcgttagc aaaaacttta ccaacgctga ttaactgaat aaaaacaaag gaaaagatag
    22681 cgacatattt actcacgttc atttgtgacc ataaaacatt tatcaaaaat ctactactag
    22741 catagcaaag ctacaattaa cataacctta atagacccaa cataaagaat aatctgaata
    22801 gctgctctat ttatttccgc ttgttcaagt taaatatttc cgcgcatcga ttaaagatga
    22861 tgcgtcgttt tctgcgatgg gaatagtcaa aaaagaaaaa ccaagtcttt tttgatgaca
    22921 aaatgcaatc aaggaaaaat tattttattt tttaacgaga tacaacaatc accttaacaa
    22981 agtatataat atatatcgtc aacctatgct catattagat tattttttga gcgcacagcc
    23041 gcagctaaca ccattgcaat taacaaattt gcatcaatcc accatcaatt tgcacacatt
    23101 attatgtgat aattgccaac cgctaaatat gcgttttgtt atctatgtat aaaaacagta
    23161 acttcaatgt cttaatggca gtttttcttg attttaatca gcattcatcg ccaatttatt
    23221 gggcatattt tttccttaag ctttaggaat tttttattat ttactttggg gcctggagac
    23281 aggaaaaatt atgctgacat tcattgaact ccttattggg gttgtggtta ttgtgggtgt
    23341 agctcgctac atcattaaag ggtattccgc cactggtgtg ttatttgtcg gtggcctgtt
    23401 attgctgatt atcagtgcca ttatggggca caaagtgtta ccgtccagcc aggcttcaac
    23461 aggctacagc gccacggata tcgttgaata cgttaaaata ttactaatga gccgcggcgg
    23521 cgacctcggc atgatgatta tgatgctgtg tggatttgcc gcttacatga cccatatcgg
    23581 cgcgaatgat atggtggtca agctggcgtc aaaaccattg cagtatatta actcccctta
    23641 cctgctgatg attgccgcct attttgtcgc ctgtctgatg tctctggccg tctcttccgc
    23701 aaccggtctg ggtgttttgc tgatggcaac cctatttccg gtgatggtaa acgttggtat
    23761 cagtcgtggc gcagctgctg ccatttgtgc ctccccggcg gcgattattc tcgcaccgac
    23821 ttcaggggat gtggtgctgg cggcgcaagc ttccgaaatg tcgctgattg acttcgcctt
    23881 caaaacgacg ctgcctatct caattgctgc aattatcggc atggcgatcg cccacttctt
    23941 ctggcaacgt tatctggata aaaaagagca catctctcat gaaatgttag atgtcagtga
    24001 aatcaccacc actgctcctg cgttttatgc cattttgccg ttcacgccga tcatcggtgt
    24061 actgattttt gacggtaaat ggggtccgca attacacatc atcactattc tggtgatttg
    24121 tatgctgatt gcctccattc tggagttcct ccgcagcttt aatacccaga aagttttctc
    24181 tggtctggaa gtggcttatc gcgggatggc agatgcgttt gctaacgtgg tgatgctgct
    24241 ggttgccgct ggggtattcg ctcaggggct tagcaccatc ggctttattc aaagtctgat
    24301 ttctatcgct acctcgtttg gttcggcgag tatcatcctg atgctggtat tggtgattct
    24361 gacaatgctg gcggcagtca cgaccggttc aggcaatgcg ccgttttatg cgtttgttga
    24421 gatgatcccg aaactagcgc actcttccgg cattaacccg gcgtatttga ctatcccgat
    24481 gctgcaggcg tcaaaccttg gccgtaccct ttcgcccgtt tctggcgtag tcgttgcggt
    24541 tgccgggatg gcgaagatct cgccgtttga agtcgtaaaa cgcacctcgg taccggtgct
    24601 tgttggtttg gtgattgtta tcgttgctac agagctgatg gtgccaggaa cagcagcagc
    24661 ggtcacaggc aagtaaatag taatgccgga ggcaagtttc ctccggcttt atcatgtcac
    24721 ccactgtggt atatgcgttg tggtctgcca actttgccgt gaacaatttc agcaataatc
    24781 agatggcggc tggcgcaata ttcaagataa cgtctggcag acgtgcggct gatggtcagt
    24841 gcctgcgcca ccgtttctgc cgtatgttgc acaccaggct ctttaaacag ttttcgcacc
    24901 gcgtttagcg tcaagggatc aatgccggtc ggcagttcgt ctttaggttc accgcgagca
    24961 taggcattaa acatctcatc aatttgcttc tggctggcgc tatcaatact ttccagcata
    25021 tgtttacgct ggcggaaacg ggttagcgtt tgccccagcc gttcataggc aatgggctta
    25081 atgagataat caaatacacc acaacgtacg gcttcagaca ccgtttccat atcgctggct
    25141 gcagtggtaa acaccacgtc gccgggataa tgcgcctgca ccagttcatg caataaatta
    25201 atccctctgc cgtcaggaag atagttatcg agcaagatta gccccggctt aaaacgctcg
    25261 atcatcattc gggcctgcgc caggtttccc gccagtaata tctgactgaa tccgggaatg
    25321 tgacgaatat attccgcatg catctctgcc agcggcgttt cgtcctcaac gatcaatagg
    25381 gttaatggag ctgtcattag gtttcacttt cggaatatag attgaaaata aggtaccgca
    25441 gggatcatta tcttcgagag tgataacacc accgcagcgc gttacgtagc tggcaatcaa
    25501 gtacaaccca atgccatgtt caccgggctc gtcagcacgc gtactgaccc cctgctcaaa
    25561 tattttgtct cgtagagact ctggaacgcc gcagccctga tcggcgactt caatcaccac
    25621 atcatcgcct tcatcgctga ggaataattc aacgatcttg tttccttcat cgctacgcag
    25681 gctggcttcg aaggcgttat caagtaaatt gcccacaatg gctgcaaact cggtgctatc
    25741 cagtcctggc ggcagttgcg aaagctggct accggggaca atgatcattt ttagccccag
    25801 ttcccgggcg cgctgcactt taccaaaaag cagccccgcc acctagcgat cggcaaacgc
    25861 ctcgcgcagg ctgtcaataa gctgttgctg ggcctgagac tccccctgca ccatcgccag
    25921 cacgcgatca tactctttca tctgcaacag accattgagc gtcgacatcc aattgagatg
    25981 ctcgtgtcgc aatgtacgaa ggctctcaac gtattgtttt atttgcgtca gttgcgcatt
    26041 gagggtggaa atttcgtctt tactgcgaaa gctgataatg gcccccagca aatcatcacc
    26101 tgaacgaata gcttcccgat tggcaataac gctcagaccg ttaaagttag ccaccatatc
    26161 ctgacgtttt tcatcaatct gttcggtaaa gaaatcggcg ggcctgacca cttcagcaat
    26221 gggtttaccc aaccattgcc gtccggggga actcagcccc agcatctttc ttgcgttacg
    26281 attgatggcg gtaatgtaac cgtgcggatc caccgcaatc agcccttcat aaaccgaact
    26341 aaacagcgcc tcttgctgac ggaccacgcg tgcgatttgc tttggctcca tgcccatcat
    26401 ctgccgacgg atatgcgcgg ccaggaacca cgacagcaac atcagaatcc ctaacagcac
    26461 gacaaacaca cctgccatcg gtaataaaaa ctcagcccgc cagctatcga ttttactcac
    26521 caggtagcca atcgacacca cgccgatgac ttttccatcg tcatcaaaga ttggcgtttt
    26581 ggcgcgcatc gccatcccca ttgacccttt cccggtaatg aagtagctct cccctttctc
    26641 cagcgcgccc tgtttggtga actgcatagg ataaccaatt ttctccggat taggatggta
    26701 aaggcggatc gagtgccggt cgccatcacc acataatcaa aatcggtatc tctttgtaat
    26761 ttgttagcga tggtcgccag ccgtttgtag tcacgcgttt tcaccgcaga gatgacactg
    26821 tcattggagg caataatttt cgcctgattc attgccatgt cgcgtacatg aagcgttaaa
    26881 tagtcctcaa aactggccgt aaaatattgc gccaaggctg caatgacaaa tattgagaac
    26941 accagaatca gcaaaaagat acgcagcgga aatgccagtc tttggaaaaa tgcaaactgt
    27001 ttattctcgt taagctgcaa cattgttttc cttgcgggtt atcccgagcc tctttgacag
    27061 atgtaattaa aatgttgaat caaaat
//
LOCUS       DAZPEB010000034        26780 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZPEB010000034 DAZPEB010000000
VERSION     DAZPEB010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26780
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.035"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..327)
                     /gene="rpnB"
                     /locus_tag="ACHURJ_001292"
     CDS             complement(<1..327)
                     /gene="rpnB"
                     /locus_tag="ACHURJ_001292"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFR1280311.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDA"
     gene            complement(524..1297)
                     /gene="hisP"
                     /locus_tag="ACHURJ_001293"
     CDS             complement(524..1297)
                     /gene="hisP"
                     /locus_tag="ACHURJ_001293"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFR1280312.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEAHERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(1305..2021)
                     /locus_tag="ACHURJ_001294"
     CDS             complement(1305..2021)
                     /locus_tag="ACHURJ_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1280313.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGIYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVLHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(2018..2704)
                     /gene="hisQ"
                     /locus_tag="ACHURJ_001295"
     CDS             complement(2018..2704)
                     /gene="hisQ"
                     /locus_tag="ACHURJ_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFR1280314.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFFGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(2794..3576)
                     /gene="hisJ"
                     /locus_tag="ACHURJ_001296"
     CDS             complement(2794..3576)
                     /gene="hisJ"
                     /locus_tag="ACHURJ_001296"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFR1280315.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(3797..4579)
                     /gene="argT"
                     /locus_tag="ACHURJ_001297"
     CDS             complement(3797..4579)
                     /gene="argT"
                     /locus_tag="ACHURJ_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFR1280316.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(4845..5414)
                     /gene="ubiX"
                     /locus_tag="ACHURJ_001298"
     CDS             complement(4845..5414)
                     /gene="ubiX"
                     /locus_tag="ACHURJ_001298"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFR1280317.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(5509..7026)
                     /gene="purF"
                     /locus_tag="ACHURJ_001299"
     CDS             complement(5509..7026)
                     /gene="purF"
                     /locus_tag="ACHURJ_001299"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFR1280318.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQDYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(7063..7551)
                     /gene="cvpA"
                     /locus_tag="ACHURJ_001300"
     CDS             complement(7063..7551)
                     /gene="cvpA"
                     /locus_tag="ACHURJ_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFR1280319.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(7810..8472)
                     /gene="dedD"
                     /locus_tag="ACHURJ_001301"
     CDS             complement(7810..8472)
                     /gene="dedD"
                     /locus_tag="ACHURJ_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFR1280320.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(8462..9730)
                     /gene="folC"
                     /locus_tag="ACHURJ_001302"
     CDS             complement(8462..9730)
                     /gene="folC"
                     /locus_tag="ACHURJ_001302"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFR1280321.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSAKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(9800..10714)
                     /gene="accD"
                     /locus_tag="ACHURJ_001303"
     CDS             complement(9800..10714)
                     /gene="accD"
                     /locus_tag="ACHURJ_001303"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFR1280322.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVEMGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPLGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLSAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(10870..11529)
                     /locus_tag="ACHURJ_001304"
     CDS             complement(10870..11529)
                     /locus_tag="ACHURJ_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR1280323.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRCSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGMLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(11612..12424)
                     /gene="truA"
                     /locus_tag="ACHURJ_001305"
     CDS             complement(11612..12424)
                     /gene="truA"
                     /locus_tag="ACHURJ_001305"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFR1280324.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAPRKDAAWTLGVNANLPDDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(12424..13437)
                     /locus_tag="ACHURJ_001306"
     CDS             complement(12424..13437)
                     /locus_tag="ACHURJ_001306"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFR1280325.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(13503..14639)
                     /gene="pdxB"
                     /locus_tag="ACHURJ_001307"
     CDS             complement(13503..14639)
                     /gene="pdxB"
                     /locus_tag="ACHURJ_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFR1280326.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIAVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            14738..15733
                     /gene="flk"
                     /locus_tag="ACHURJ_001308"
     CDS             14738..15733
                     /gene="flk"
                     /locus_tag="ACHURJ_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFR1280327.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(15730..16908)
                     /locus_tag="ACHURJ_001309"
     CDS             complement(15730..16908)
                     /locus_tag="ACHURJ_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1280328.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPLKFALLVVGRLILGFGESQLLTGALTWGLGIVGAKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(17201..18421)
                     /gene="fabB"
                     /locus_tag="ACHURJ_001310"
     CDS             complement(17201..18421)
                     /gene="fabB"
                     /locus_tag="ACHURJ_001310"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFR1280329.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            18580..20586
                     /gene="mnmC"
                     /locus_tag="ACHURJ_001311"
     CDS             18580..20586
                     /gene="mnmC"
                     /locus_tag="ACHURJ_001311"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFR1280330.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQQDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIAC
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(20707..20985)
                     /locus_tag="ACHURJ_001312"
     CDS             complement(20707..20985)
                     /locus_tag="ACHURJ_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFR1280331.1"
                     /translation="MIAEFESRILALIDGMIDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(21019..21567)
                     /gene="epmC"
                     /locus_tag="ACHURJ_001313"
     CDS             complement(21019..21567)
                     /gene="epmC"
                     /locus_tag="ACHURJ_001313"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFR1280332.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(21567..22376)
                     /locus_tag="ACHURJ_001314"
     CDS             complement(21567..22376)
                     /locus_tag="ACHURJ_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFR1280333.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFAGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(22376..23200)
                     /gene="mepA"
                     /locus_tag="ACHURJ_001315"
     CDS             complement(22376..23200)
                     /gene="mepA"
                     /locus_tag="ACHURJ_001315"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFR1280334.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFAPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(23204..24289)
                     /gene="aroC"
                     /locus_tag="ACHURJ_001316"
     CDS             complement(23204..24289)
                     /gene="aroC"
                     /locus_tag="ACHURJ_001316"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFR1280335.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGILLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(24324..25256)
                     /gene="prmB"
                     /locus_tag="ACHURJ_001317"
     CDS             complement(24324..25256)
                     /gene="prmB"
                     /locus_tag="ACHURJ_001317"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFR1280336.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPDAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            25422..25973
                     /gene="smrB"
                     /locus_tag="ACHURJ_001318"
     CDS             25422..25973
                     /gene="smrB"
                     /locus_tag="ACHURJ_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFR1280337.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(26046..>26780)
                     /locus_tag="ACHURJ_001319"
     CDS             complement(26046..>26780)
                     /locus_tag="ACHURJ_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFR1280338.1"
                     /translation="EFEFHLIIATPDSVNYAIFKATFMPNSQPDLVSWTGDSSTQPSM
                     SKISDSRVSMSACPGLEQYDSQTKTGWTCNELKMFVYYDGNLHGCPWIVSSFVKSRDP
                     FAKTYDDDFPDYIGPTKVSSSCPAVPLAPYDVSWNENYVVHNKVVRLQSTGGVIEQTL
                     PTFLMENGKLCNGNNFDERGVYCRFIAQQMTFSTSGCDNAKVTVTPEPQPITSRQLHD
                     MKLRVDTTSRQPIDSTCRFTYILNMY"
BASE COUNT         6602 a         7321 c         6758 g         6099 t
ORIGIN      
        1 cgcatcaaga tggttttgca ttgccgcaat ggaataacgc atcatgcgaa aagccattaa
       61 ttcttccggc ttactttggt gctctatcac tacataaata taacccactc cctcctgcgt
      121 tttcacagac cacaagaggt cggaataata ttgccgcagg tcttcatcaa taaaactgtt
      181 tggttccagt ttaagcgtcg ttaaatcaca cagtttgcgc agcggcgcgg gaagatgaat
      241 atcaataaaa tcccgcgcgg tgtctggatg gcgtaaaaaa gatttaaata ccgcatcatg
      301 cggcgtggaa gttgtcgata ttgtcatggc gttccgtcac ccctcaaatt taagatgacg
      361 cgacaataac cacgattaac acccaatgca gcaggcagtt tttatctttg gacagcgaat
      421 tcagaagtat ttttagcgga ctgcaacttt tacatttcag cggaagctga ctcgcaaatt
      481 ccccatgaaa atactccggc agcgttagcc accggagagg aaattatttc agcgatccct
      541 taaggaactg ttgcagacga gggctttgcg ggttgccaaa taactgctcc ggcgcgccct
      601 cttcttctat tttcccctga tggaggaaaa tgacatgagt agaaacatgg cgagcaaagc
      661 ccatttcgtg agtcactacc accatggttt tcccctcttc tgccagttgc tgcataatac
      721 gcaacacttc gcctaccagt tcaggatcga gcgccgaggt aggttcatca aacagtaaaa
      781 cttccggttc catcgccagc gcccgcgcga tagaaacacg ctgttgctga ccgccggaaa
      841 gatgcaccgg atatttcccc tgcgcacgtt cgtctatccc gacttttgcc agatacttga
      901 ccgcccgctc gtgcgcttcc tgcttgctca ggcccaacac ctgaatcggc gcttccatga
      961 cgttttccag caccgtcata tggctccaga gattgaagtg ctggaatacc atcgtcaggc
     1021 gtgtgcgcag taagcgcagt tgatttttat cggcgacttt gagttgaccg tctttgtcgc
     1081 gcaccagatt gatcgtctgg ccattgacca cgatcgaccc ttcactcggt ttttcgagga
     1141 agttaatgca gcgcagaaag gtacttttcc ccgatcccga cgatccgatg atgcttatta
     1201 catctccggc attcgcttgc agtgagaccc ctttcagcac ttcatgttcg ccgtagcgtt
     1261 tgtgcaaatc gataacgttt aatttattct cggacatcgt attctcagtg cgttgaagaa
     1321 ggtttcacat gctgcaacca gcgtttttcc gctctgcgaa agaggctgat caggacataa
     1381 gagatgatta aatagagcac cgcggcaatg ccgaaggcgg taaaaggttg atacgtggcg
     1441 gcgttaatat cgcgggctat tttcagcaga tccggcacag tggccgtaaa cgccagcgca
     1501 gtagagtgca gcatcaggat cacttcgttg ctgtatgccg gtaacgcaat acgcagcgca
     1561 gaaggcaaaa taatgcaacg atacatttta aaagtcgaga agccataggc tctggcggct
     1621 tcaatttccc catgcagaac cgaacggatt gccccagcaa aaatctcggt agtgtaagcg
     1681 caggtgttaa gcgtcagcgc cagcacggta cagttcaggc cactgcggaa gaaagcgtta
     1741 aggaattcgg ttcccttaac aatctcaagc gtgtatatgc cggaatagaa caccagcaac
     1801 tgaacataca gcggcgtacc gcgaaaaata taggtaaata accagattgg aaactggatg
     1861 tatttattac tggagacacg accaatcgcc agaaacagcg ccaggactcc gcctatcact
     1921 accgacaaaa taagcagcca cagagtgatc gccacaccag taaagcgata accgtcggtc
     1981 cacagcagcg gtttccagta ttcatgtaag atttcgatca caggtcagcc ctcttcacac
     2041 ccacggagta gcggcgctca aggaacagca gcacaccatt ggaaacagtg gtgaaaacca
     2101 ggtaaatcac gccacagacg atggcgaaat agaacggttc ccaggtactt ttgcctgcca
     2161 gttgggtggc tttgaccaca tcttccaggc cgagtaacga aaccagtgcg gtagatttga
     2221 ggatcacctg ccagttgttg ccaatgcctg gcagggcgta acgcatcatc gccggaaaca
     2281 tgatccgccg aaacacttgc ccacgagtaa aaccgaacgc cgtcgccgcc tctatatgtc
     2341 ctttcggcac tgccataaaa gcaccacgga acgtttcggt aaaataagca ccgtagataa
     2401 aaccgagagt gataatacca gcgaccatcg gatcgatatc aatctgcccg acgcccatcg
     2461 cctccgtcac cgtgtttagc gcaatctgca aaccgaagaa aatcagcagc atcaacacta
     2521 agtccggcac gccacgaatc agcgtggtgt acccttcgaa aatcagcccc gaaagccgat
     2581 tttgcgagag cttaccgcca gcgccaatta aaccgatgat tacagcgagc actacagagc
     2641 tgatagccag ctccagcgtg acgagcgccc cctgtaaaat aacacctgaa aacccatata
     2701 acatgctgcc tgtcctgtcg tgtgtggtga attaccgcct cttgtctccc tccggttacc
     2761 cgaagggagc gggctttctt tcaccgatgg cgattagcca ccataaacat caaaatcgaa
     2821 gtactttttc gctaatttct cgtaagtacc gtcagcgcgc atttcggcaa aggctttgtt
     2881 cagtgcttcg cgcagttcgt tatcttcttt acgcaggccc atgccggtcc ctacgccaaa
     2941 cagtttttca tctttaacag acgggccacc gaatttgtaa tctttaccga caggttgttt
     3001 gaggaaacct tcgctggcag cgacctcatc ctggaacgcg gcatcaatac gtccggcagt
     3061 caggtcagaa taaatgttgt cctgcccctg atacgagacg atttcaatgc cttttggtgc
     3121 ccaatgttca ttaccgaacg tctcctgggt ggtgccctgc aatacgccta cccgtttgcc
     3181 tttcagcgac tcgactgtcg gctgaatgtc agaatttttc gccaccacca aacgagaatc
     3241 ggcagcgtac agtttgtcgg tgaaggctat ttcttgctga cgtttttccg taatggaaag
     3301 cgatgacatg atggcgtcaa tcttcttcgc ttttaaggac gggattaacg catccagcgg
     3361 attttcgaca aacgtacatt gcgtattgat gcgtttgcat aattcctttg ccagatcgat
     3421 atcgaagcca accagttcgc cttgtgaatt ttttgattca aatggcgcat aggtcgggtc
     3481 ggtaccgatg cggatgtttt gcggaatcgc agcaaatgcc gcagttgcgc tggagaaggc
     3541 cagaaccaga gagagcgata gcaccagttt tttcataatg tcctcaactg acagtctttt
     3601 tatagggaat atttacaggt ttgttacact tatcgtgcca taaaattgac catcaaggca
     3661 aaatatcccg ccccgttaga tgtattattt ttcattaaaa atgcttaagt atgcatttca
     3721 atgcaccacg atagtgcaac aaacctagcg tgcaccatga tgatcattcc atcaggtaca
     3781 gcttcccagc gacgtatcag tcaccgtaga cattaaagtc gaaatacttt ttcgccatct
     3841 tgtcgtaggt gccgtcctga cgcagctcgc caagcgcctt attgaaggca gccgtcagtt
     3901 cagcatcatc tttacgtagc cctacaccgg tgccatcacc gaagtatttt ttgtctttta
     3961 ctgatgagcc agcaaaggcg aaatctttac cagcaggttg cttgaggaat ccttcgctgg
     4021 cagcaacttc atcttgtaac gcagcatcca gacgtcctgc agccagatcg gaatagacca
     4081 aatcctggtt ggcataggcc accacatcca cgcctttgct acgccaggtc tcgttagcgt
     4141 aagcttcctg ggttgatccc tgcagcacac caacatgttt acctttcagt gaatccagcg
     4201 ttggctgaat cggtgaacct ttggcagcaa tcaaacgaga atctgcggcg tacagcttgt
     4261 cggagaaggc aatctcctgc tgacgtttat cggtaatgga aagcgacgaa ataatggcgt
     4321 cgattttttt cgctttcagt gaggggatca gcgcgtcaaa gtcactggca acccaggtac
     4381 atttcacctg catccgtttg cacatctcgt taccgagatc gatatcaaag ccaacaaaat
     4441 cacctttagc atctttcgac gagaacggcg cataggtggt atcggttccg atacgtaccg
     4501 tctccggtag cgccgcatag ctggaagccg ctgcggagag accgactaac aaagacagag
     4561 cgagaatcga cttcttcata cataaccctc aagtgaaaat ggctttttta tgttgtgtat
     4621 tgtgttgtgt gtttgcaggc tctttcatgc aggtcttatg ccatcttgac gacaacagta
     4681 acattcaacg ttaaatatgt taataagacg ttgcattatt gtcctgaagt tgaagatagc
     4741 aggtatagcg gttgaatcgc agcgtttcga ttgaatggca gaaacaaaat gtcgaggatt
     4801 tgatcgcggt tgcaaaattg ccctgaaaca gggcaacagc ggagttatgc gccctgccag
     4861 cgggcaaaga gatcttcagg aagggttatt gcaaactggt caagaacacg attaaccgtc
     4921 tgatttatca catcatcaag ggattgcggg cgatgataaa acgccggaac aggaggcata
     4981 atcaccgcac cgatttctgc cgcctgagtc attaaacgca gatggcctaa gtgcaatggt
     5041 gtttcacgca cgcagagcac caacgggcga cgctctttca gcaccacatc tgccgcacgg
     5101 gtcagtaagc catcagtata gctatggaca atgccggaaa gggttttgat tgaacagggt
     5161 aaaatcacca tccccagcgt ctggaaagaa ccggaagaga tgctggcgga aatatcgcgc
     5221 gcatcgtgcg tgacatcagc taatgcctgc acttcgcgca gggaaaaatc cgtttcgagg
     5281 gataaggtct ggcgcgccgc ctggctcatc accagatgcg tttcgatatc tgtgacatcg
     5341 cgcagaacct gtaataagcg cacgccataa atcgcgccgc tggcaccgct gatgcctaca
     5401 atgagtcgtt tcataaatag ttgccctgtc agacttgcag gcagactttg cagtatttcg
     5461 gcgggagttg caagtcaggg tgccagaccg gcaccctcag cgaaggcatc atccttcgtt
     5521 atgcatttcg agattttcca cttcgttctg acgttgcact gctttggcgt catcattacg
     5581 taacgtatcg aggaaatcga ggtagtcctg atcaacatct ttggtgacgt agacgccgtt
     5641 gaacaccgag cattcaaact gctggatatc cggattttca gcgcgaacgg cgtcgatcag
     5701 atcgttcaga tcctggaaaa tcaacccgtc agcaccgatg atctggcgaa tttcatcaac
     5761 ttcgcgaccg tgagcgatca gttccgtggc gctcggcata tcaataccat aaacgttcgg
     5821 gaagcgaatt tccggggccg cagaagcgag gtacactttc ttcgctccgg cttcgcgtgc
     5881 catctcgata atctgctcag aagtggtgcc acggacgatg gagtcgtcga ccagcaggac
     5941 gtttttatcg cggaactcgg cgcggttggc gttcagttta cggcgcacgg acttacgacg
     6001 cagctgctgg cccggcatga tgaaggtgcg gccaacatag cggtttttaa cgaagccctg
     6061 gcggtacggc ttgcccagaa tacgagcaat ttccagcgcg atatcacacg aggtttccgg
     6121 gatcgggatc accacgtcga tatccagatc ttcccattcg cgggcaattt tctcacccag
     6181 tttcgtgccc atattcacac gcgcgctgta aacggaaatt ttgtcgataa acgagtccgg
     6241 gcgggcaaag tatacatact caaacaggca cggattgctg accggattgt cagcacattg
     6301 acgggtaaac aactgccctt cttcagtgat gtaaatcgct tcgcccggcg cgacgtcacg
     6361 caggaaatca aagcccagcg tatcgagcgc tacgctttcg gaagcgacca tatattctgt
     6421 acggttctcg tcaatatcac gttttcccag taccagcgga cgaatcccgt ttggatcgcg
     6481 gaaagcaacc ataccgtggc cgataatcat cgccacacag gcatatgcgc cgcggattaa
     6541 gcggtttgtg gcagcaatgg cagcgaaaat attgtcggct tccagcgggt agtggcggaa
     6601 gttgtccagc tcgctagcga agatattaag cagaatttcc gagtcggaag tggtgttgat
     6661 gtggcggcgt ttttcttcaa acagtttttt acgcaactcg tgagcgttgg tcagattgcc
     6721 gttgtgggca agcgtaatgc catacgggga gttaacgtaa aacggctgcg cttcagaggc
     6781 gctggagctg ccagccgtag ggtaacgcac atgaccaatg cccatattgc cctgcaaacg
     6841 ctgcatatgg cgagcttcaa atacatcgct caccagcccg ttcgctttac gcaaacggaa
     6901 gcagttattg gcatctatgg tgatgatgcc ggcggcatcc tgaccgcgat gctgaagcac
     6961 cgttaaggca tcataaatcg actggttaac cggcataaca ccggcgatac cgacaatacc
     7021 gcacatacgt ctttttcctc gttaagccac atctcagagc acttacgctc tgggcaagaa
     7081 acttgacgag ctttgcagat aatcaaaaaa ccatctgatg ataaaactga actgcgggat
     7141 cagctgtgat ttgctccagt cttcgctttt cgacaccccg gtaaaggagt cgagaaagaa
     7201 gagaatggcg gcaacaatca acacaccgcg caacgcaccg aaacagacgc ccagcacccg
     7261 atcggtgcct gacaaccccg ttttctccac caactggcct atcacgaagt tcacgatagc
     7321 accaacgatc agggtagcga taaacagtac cgcgatggca attccatttc gaaccagttc
     7381 gtcttcaaag cccgtaaacc agactgacag gtaagtgtag taatgactgg caacaaagaa
     7441 agcacaaccc catgtcacca gcgataacgc ttcacgaaca aagccgcgga tcaggctaac
     7501 cagagaggaa aaagcaatca ccgcgattat ggcgtaatca atccagacca tatgtgtccc
     7561 acgattttac gccctgtcat cctgttcggg gcgcattcta acagaaaaag aaaacgtttg
     7621 cgtagggatt tccttcccac gcatcaataa aaatggcgct gaaaaaatat tcaacgccat
     7681 cgacttttta tgcctttgcg gcatcgtgca atgcgtgtcg gatgcggcgt aaacgcctta
     7741 tccgacctac ggttctaaca ctgcccaagc ctgataagac gcgccagcgt cgcatcaggc
     7801 aagactgtat taattcggcg tatagcccat taccacgcca cttaagccag aaagttgctt
     7861 caactcaccc agcgaacctt tcagcttatc tttcgaggca tccggcccaa ccagaatacg
     7921 ggtaatttta ccctgcactg gcgtggatgg cgacgtataa acccgataac cggcaccgcg
     7981 cagcttaccg acaatctcat tcactttatc ggcatttttc agcgcaccca gttgcacaac
     8041 ataggcttta cccgttggcg ctgctttttc ttccacgacc ggcttcggtt ctggcttcgg
     8101 cgcaggtggt gcttcaacct tcggtttagg cggctccacc ggtttcggct tcggtggggc
     8161 gaccggtgca ggttccggtt caaactcggt gttattggct gcaatagtgg ccggatcgag
     8221 cgacggtgct gcggcatcac ctgcccgcac ctcttccgct gcgccttccg gcggctgcgt
     8281 cggcaacgcc tgggtggcgg ctggcatcat atcaggctca tcgcgatcgc ccgcttttgg
     8341 aaccagcggg atagccgcaa actcatcctg ataatgtttt ttctgcccgt ccagcagccc
     8401 tggaagtaca atcaccccca gcgccaccag cacgatcgtg cccactaacc gattctgaaa
     8461 cttacttgcc accgcttctc ctcgcgtcaa tcacttccat gacatgtgcg accgtgtgga
     8521 aagaaccaca caccagcacg gtgtcttccg ctttagcgtc cgccattgcg gcatcccatg
     8581 cctgcgcaac gctatcaaat gatttgccgt tacccaaatg ctcaagcagt tgttctgccg
     8641 tggcaccgcg cggcccttcc agtggcgcac aataccagtc atcaaccacg cttttcaacc
     8701 aggccagagt tccggcaata tctttatcat gtagcatacc gataaccgcc agcacgcgcc
     8761 cgtttttcgg tagcgctttc atacgcccgg tgagatattc cgccgcatgt ggattatgcg
     8821 cgacatcaaa aataacgcgt ggcgactcgc tcacaatctg gaaacgtccc ggcaaaattg
     8881 cgctggcaat cccgtcgcga atggcatttt cactgacttc cagcccgctg gcacgcagtg
     8941 ccgccagcgc tgttgcggca ttcggttgcg ggacaagcgg caacggcaga ttttccagcg
     9001 tgccgtgagc atcgctaaac gcccagtcat gatcggtgac ggaatagttc cactcaacgc
     9061 cccgacgttg taacagtgca cctttttcct gcgccacatc agcaatggta gaaggcattt
     9121 ccggctcacc gacaattgcc ggttttgcgc tgcgaaagat gcctgctttc tcgcgaccaa
     9181 tactttcgcg atctggcccc agccagtcgg tatgatccag cgcaatactg gttactaccg
     9241 cgacatcggc atcgacaata ttggttgcgt ccagacgacc gcccagccct acttccagaa
     9301 tcaccacgtc aagttgtgcc tgtttgaaca gccacaacgc cgacagcgta ccgtactcga
     9361 aataggtcag ggaaatatca ccgcgtgccg attcaatctc cgcaaaagag gcggtgtggg
     9421 ccgattccgg caattcctgc ccctgcacac gtacgcgctc ggtataacgc accagatgcg
     9481 gcgaactgta gacgcccact ttgtaccctg ccgccatcag aatcgactcc agcgtacggc
     9541 aggtggtgcc tttgccattc gtacccgcaa cggtaaacac aaatggcgct ggtttcagaa
     9601 cgccaagacg agccgcgacc tggctcacgc gctcaaggcc gagatcgata gttttactgt
     9661 gcaggttttc cagataagaa agccacgaag ccagaggcga cgcggcttga ggagtgcgtt
     9721 tgataatcat ggtatccgct gattcgttac ggtgagaata gcaaaagggc agagccagtg
     9781 gccctgccct tatcagttat caggcctcag gttcctgatc cggtaccggg ggtaccacta
     9841 cgccttcacg cggcgcttca ggattcggcg ctgacagatt catcaacttc gccagaatgc
     9901 tcgccagttt caggcgcatt tccggacgac ggacgatcat gtcgatcgcg cctttctcga
     9961 tcaggaattc actgcgctgg aatcctagcg gcagtttttc gcgaacggtc tgttcgataa
    10021 cacgcggacc ggcaaagcca attaacgctt tcggttcagc gatgttgaga tcgcccagca
    10081 tggcgaaact tgcagaaaca ccgcccatcg tcgggtcggt cagcacggag atgtacggca
    10141 agccgcgctc ctgcattttt gccagtgccg cagaggtttt cgccatctgc atcagcgaca
    10201 tcagtgcttc ctgcatacgt gcgccaccag aggcggagaa gcagatcagc gggcagttat
    10261 cttccagcgc ctgctcaacg gcacgcacga aacgtgcacc cacaacagac cccattgaac
    10321 cgcccataaa ggcgaactcg aatgccgcag cgacaaccgg cattccatac agagtgcctt
    10381 tcatcaccac cagcgcatct ttttcgccgg tttctttctg cgcagaagcc agacggtctt
    10441 tatacttctt ggagtcacga aacttcagca catctttcgg ctcaagctcg ctacccatct
    10501 ccacaaggct tccttcatct aacaggctat gcaggcgatt acgcgctgtc atacgcatgt
    10561 gatggtcaca cttcggacag acctcaagat tacgttccag ctcagcgcgg tataaaacct
    10621 gaccgcagct atcacactta gtccacaccc cttcaggaat gctcgccttg cgggtgggag
    10681 taatgttgct tttaattcgt tcaatccagc tcattaggga cctttctgtc tgaacctggt
    10741 tcgatgccag ttttatcttt ggggacgcat aatgccattt ttgcccccaa cagaccatga
    10801 atgttgcaca ttaaaacata acagcccgaa actttggata aaaaagtggt cgaaccgcgg
    10861 agttactttt tattttgcgg cgcgtgccgc agcgcgtttg tgacgaataa attcgaaaac
    10921 cccaggcaat attgaaacaa aaataatgcc aacaatcatc agcttaaggt tgctctgaat
    10981 gaaggggagc atgccgaaga aataacccgc gtaggtaaaa atcagtaccc acaacagtgc
    11041 gccgatcacg ttataagcgg cgaaatgacg gtacgacatg tggcccattc ccgcaacaaa
    11101 cggcgcgaac gttctgacga tcggtacaaa acgggcgaga ataatcgttt tgccgccatg
    11161 tctctcgtaa aactgatgag tcttatcaag ataactgcaa cgaaagattt tcgaatccgg
    11221 gttactgaac agtttgtcac caaataaccg tccaatggta tagttcactg catcaccgac
    11281 aatagcggca atcaacataa gcaccaccat catatggaca ttgagatcgt tagtttccag
    11341 cgacgccaat gcaccagcaa caaacagcag cgaatcacca ggcaggaatg gcgttaccac
    11401 cagccccgtt tcgcagaaca gaatcaaaaa cagaatggca taaacccaga cgccgtactc
    11461 cgcgaccagc tctgccagat gcacgtcaat gtgcaggata aaatcgatga ggaaataaat
    11521 caggtccata tttgcctatg ccttgtactc gtcatctttc aggctgtaac tacgttggct
    11581 acagcctgaa atgttccgag cattattctc gttagtccgc cagaaatagc gggcccattg
    11641 gcggttttgg aagatcatac cggtcagggt aatccaccgc gaccagatac agcccttccg
    11701 cttttgccgt tgctgccgcc agcgttctgt cctttgccgc cagcaactct gctatccagc
    11761 tctccggctg gttgtgggca ccgacttcca tcaggctgcc gacaatattc ctgaccatat
    11821 gatgtacaaa ggcattcgct ttgatatcta ccaccacata aggaccgtga cgcgtgacat
    11881 taatatgcat aacgttgcgc cacggggttc gggactggca ctgcaccgca cggaacgagg
    11941 tgaaatcatt ctcgcccagc aagcattgcg cagcccgatg catccgttca gcgtccagcg
    12001 gttcgtaaaa atgggttacc cctttactca gtaccgccgg gcgcagccga tgattgtaga
    12061 tgatgtagcg ataacggcga gccgtggcgc taaatcgggc atgaaaatca tcaggtacag
    12121 ctttaaccca acgcacggcg atgtcatcag gtaaattcgc atttacgccc aaggtccacg
    12181 ccgcgtcttt gcgcggcgcg gttgtttcga aatgcaccac ctgcccggta ccgtgtaccc
    12241 ctgcgtcagt acgcccggcg cagaagacgg tgatgggttc gttcgccacc tgggagagcg
    12301 ccttttccag cttctcctgc acactgcgga cttcattctg ccgttgccag ccgtaatact
    12361 tactgccgtc gtactcaatg cccagcgcaa ttttataaac tggcagttgt tgctggtcgg
    12421 acattagtac agatactcct gcaccagttt ctcggcgatt tttactgcca tcagcgcgcc
    12481 gccaaagcga acgttatcgg ccaccgacca gaactggact tgctccggca taccgtagtc
    12541 attacgcacg cagccaacag aaagatgcgg agtacccgaa gcatccccta cctgagtcgg
    12601 gaattcgttc tcttcagaga gcacaatatc ttcgccctga gcaaacgcat cacgcgcttc
    12661 ttctgccgcc agcggacgca gtgcttcaaa gttgaccatc tgggcatgac cgtagaatac
    12721 cggtgcctgg acgacgcttg ccgaaatcat cagcccttcg tcctgcagga ttttgcgtac
    12781 ttcgtcaacg atacgacgtt cttcacgcac gctaccttcg ctatccggca acaacggcag
    12841 catattgaac gccagttgac gaccgaagaa atcttcttcg tcaatcggaa tgccgttgag
    12901 caatttcgca ctctgccccg ctaacgcatc gaccgctttt ttgccctggg cggaggctga
    12961 aatcaggctg gtaacactga tacgcgacaa accgccctga tcgattaacg gtttcagtgc
    13021 cgccagcagc tggctggtca gactgtctgg tacggcgatg acattccggt tgcggtaatc
    13081 ggtcagtaca aacgggttta cttccggcac caccagcggt acgtcgggtt cgagagcaaa
    13141 cagtccgctg ctgtcgatca ccaggcaacc tgagttggtc gcttcttcaa cccaggcagc
    13201 ggtagcttct ttgcctgcga caaaaaatgc cagctgcgcc tgcgtccagt cgaattcagc
    13261 ggcatcctgc acggtgattg ttttaccacc aaagcgcagt tgttcgcctg cgctttcgtt
    13321 acgtgccagt gcataaattt ccccaaccgg gaactgacgt tcagccagcg tttcaagcag
    13381 ggcttcgccc acagcgccag ttgcgcccag gacggcaatg ttccagcctt cagacatggt
    13441 ggtttactcc agaaatagca aagctccctg ccaatgttac agcagagagc atgaagaaga
    13501 gattaacgtg ccggatgatg aacggcgtta aaacccagtt tacacagcaa tgatgccgca
    13561 ctggcgtcat cacaaattac atacagagat gaccattcac ggcgctcaag atagtttttg
    13621 cgcagtttat cgaactcacc cggtatcccg gcgactttac gcagcggtgc gtcatcgcgg
    13681 cgcacatcat acaccaaatg caccagcctt ttcagcgtcg gttgatcgag cgggccatgc
    13741 agcgtaatgc gaccaaactc tggcgcgggc agtaatgtat ccagcgcaac gtgctgttca
    13801 tgcccaataa acttgctata agcttcaaac acttgcgtag taccgcgtgc tttaccttcc
    13861 agggtataac ctgcgatatg cggcgtgccg atatccactt ttgtcagcaa ctccacgtta
    13921 agttccggtt cgccttccca gacatccagc actacgctta acttctggcc ttcattcagg
    13981 caggtcagca acgcagtatt atcgacgact gcgccacggc aggcgttaat cagaatcgct
    14041 cccggcttca ggctacggat cagtttttca tccgccagat gcagcgtttt gtacggccca
    14101 tctttgaaga gtggcgtatg gaaagtcaga atatccgcat gctgaactaa ttcatccagc
    14161 gagcggaaat caccctcatc cccacggtca gcgcgaggcg gatcgcaaag caatgtttta
    14221 atccctaacg cttccagtcg cgcctgcaat cgacgtccaa cgttaccaac gcccacgatc
    14281 cccaccgtac ggtcgtgcag tgaaaatcca tcgcgttcgg caagcatcag tagtgaggag
    14341 aaaacatatt ccaccaccgc aatcgcatta cagccaggtg cagcggaaaa accaattccc
    14401 gcctgcttca accatgcttc atcgacatgg tcggtccccg cagtggcagt gccaacaaat
    14461 ttaatgggtt ttcctgccag caaagattca ttcactttcg tgaccgaacg caccatcagc
    14521 gcatccgcgt ctgccagttg agcgacggcg attggacgcc cgggaaccgc ggtcacctca
    14581 cccaaacggc taaataagtc gcgggcataa ggcatatttt catcaacaag gattttcacg
    14641 tttgtgttac ctgtatgaga cgagagttaa ccggacaagt gtgccataat ctcgcggcca
    14701 ggcatacttg cgaagatttc aggtataagg atacgtaatg atacaaccta tttccggccc
    14761 tcctcctggg caaccaccag gtcagggaga taacctgccg tctggcgcgg gcaatcagcc
    14821 tttatccagt cagcaacgta cttcgctgga aagcttaatg acgaaagtga cctcactgac
    14881 gcaacagcaa agagcagaac tgtgggcggg tatcaggcac gatattggtc tgtcgggaga
    14941 ttcaccgctg ctttcgcgtc acttccctgc cgctgagcat aatctggcgc aacgtctgct
    15001 ggccgcgcaa aaaagccatt ctgcccgcca gcttttagcg caattagggg agtatttacg
    15061 tctggggaat aatcgtcagg cggtcacgga ttatatccgt cataactttg gtcagacgcc
    15121 gctgaatcag ctctcaccgg agcaattaaa aaccattctc accctgttgc aggaagggaa
    15181 gatggttatt ccgcaaccgc agcagcgcga ggcgaccgac cgtcctttat taccggcgga
    15241 gcacaatgcg ctaaaacagc tggtgaccaa acttgcggcg gcaacggggg aacccagcaa
    15301 acagatctgg caatcgatgc tggaactttc cggggtgaaa gatggcgagt taattccagc
    15361 gaaactgttt aaccatctgg tgacctggct acaggcgcgt cagacgctaa gccagcaaaa
    15421 tacgccgacg ctggaatcac tacagatggc gctaaaacaa cctttagatg ccagtgaact
    15481 ggcggcgtta tcggcatata tccagcaaaa atatggcctt tctgcgcaat catcgctttc
    15541 ttctgcccag gccgaggata ttcttaatca gctttatcaa cggcgggtta aagggattga
    15601 tccgcgtgat atgcaaccgc tgcttaatcc ttttccaccg atgatggaca cgttgcaaaa
    15661 tatggcaacg cgtcccgcgc tgtggatact gttagtcgcg attatcctga tgctggtctg
    15721 gctggttcgt taaccccgac gaaatgacag tatcgtgaca ataataccca gcaccgcaga
    15781 gatcgccccg gcaagaaata ccgaagagta accaaacgtg gtcgccagca ttcccgccag
    15841 cggcccggag acgccgaggg cgatatcctg aaacgcggcg taaccgccca gtgcggtgcc
    15901 gcgaacttgt gaggggacgc gtttaaccac ctccacgccc agcgcaggaa agataagcga
    15961 acatccggct ccggttaacg ccgcgcccgc taatgcgacc catgcacctg gggcttgcca
    16021 gagcagcaac aagcccaccg tttctacaag cagagagaca atcgccactt tcacgccgcc
    16081 aaaacggtcc ggcatccagc caaacatgac gcgcatcacg acaaatgcgc cgccaaacgc
    16141 ggtaagagta aagcccgcca tcgcccatcc tttgctggca aagtagagcg aaacgaaagt
    16201 cccgataacc gcaaaaccaa cgccttgtag tgccagccct aaccctggtt tccagataag
    16261 cccgacaacg ctccacagcg atggacgttc tcccgccagg gccggtactt tgcgcactgt
    16321 gccgttacag gcccacgcca gtaagggtaa taccattgtg gtgatcgcca gtgcggcaaa
    16381 accgtaatgg ctatgaatca acaggccaag cggcgcacca acagcgaggg caccgtaaat
    16441 cgccattccg ttccatgaca tcactttgcc agagtgtttt gcccctacga tgcctaatcc
    16501 ccaggtcaga gcgcctgtca gtaactggct ttcaccaaag ccaagaatca aacgcccgac
    16561 gaccaacagg gcaaatttca acggtgcgga gacaggcaaa atcgccgcca gcagcaacgc
    16621 gccgccagcc agaccacaag ctaacatccc ctgaagcgcc gaacgttttg caccatattg
    16681 atcggccagt cgcccggcat agccacgcgt cagcaccgta gccagaaact gaatcccgac
    16741 ggcaatcccg accatggtat tgccatagcc cagatcatga tgaacaaaca gcgggataac
    16801 cggcaacggc agccctacgg tcatgtaggt gagaaaaacc gcaaaagcga tgcggaagag
    16861 cgaaaaattg gcagaagatc gtgtttcggt ttggcttaca gcagtcatgc attactccag
    16921 aatgcagcgc aaggcgagga gtatccccgt ctcatctctc tggtttcagg gttacagtgc
    16981 gttggcagga tttaacgcgt acgtcttttc agaaggaaat cgacaaagcg ggaagtttgc
    17041 ctggaactgg cggcgattgt caatgatgtg aaaaagggaa ccatcaggtt cccttttgcg
    17101 ttagtgccgg aggccgcatt ggcgcgtaac gtcggatgcg acgctggcgc gtcttatccg
    17161 acctactccg actactccga ctactccgac ctactgcgaa ttaatctttc agcttgcgca
    17221 ttaccagcgt ggcgttggtg ccgccgaagc cgaagctgtt agacataacg gtggtcagtt
    17281 cgcgatcggt cgtttcggtc acgatgttca gacctgccgc ctgctcgtcc agctcttcaa
    17341 tgttgatgct cggggcgata aagccgtgtt ccagcatcag cagagagtag atagcttcct
    17401 gtacgccagc agcgcccaga gagtgaccgg tcatggcttt ggttgcagaa atcgccgggc
    17461 tcttatcgcc gaacacttca cggatagctg ccagttcttt cacgtcgcca accggagtcg
    17521 aagtaccatg agagttcagg taatcgattg gggtatcaac gccgtgcatc gccatcttca
    17581 tgcagcgtac tgcgccttcg ccagacggag caaccatgtc tgcaccatca gaggttgcgc
    17641 cgtagccaac gatttcagca tagatgtgag caccacgcgc cagcgcgtgt tccagctctt
    17701 caaccactac cataccgccg ccgccagcga taacgaaacc gtcacggtga gcgtcgtaag
    17761 tacgggaggc tttttccggg gtgtcgttgt atttagtaga cagcgcaccc atcgcgtcga
    17821 attcgcaggc catttcccag cacagctctt cgccgccgcc agcaaacacg atgtcctgtt
    17881 tgcccagttg gatctgctct actgcgttac cgatacagtg tgcggaagtc gcacacgcgg
    17941 agctgatgga gtagttaacg ccatgaattt taaacggcgt ggcgaggcag gcagaaacgc
    18001 cggatgccat cgctttggtg accacatacg ggccaaccgc tttcaggccg cgcgggccgc
    18061 gcattgcgtc agcgccgaat acctggaaac gcggggaacc gccgccagaa cctgcaatca
    18121 ggccaacgcg cgggttattc tggtaagctt ccggagaaag gcccgcatct gcgattgcct
    18181 gctccataga aaggaatgca taaatggatg cgtcgctcat aaagcgcaca actttgcggt
    18241 caatgaggcc agtggtatcc agttttacgt tgccccagac gtggctacgc atgccggaat
    18301 ccttaagctc ctgagagaaa gtgatccctg aacgtccttc acgcagagat gccaggactt
    18361 cctgctggtt attaccgatg ctggaaacaa tgcccaggcc agtaatcact gcacgtttca
    18421 ttcaatacct ctgtaagtcg cacatagagt aagtttcgaa tgcacaatag cgtacacttg
    18481 tacgccgaac aagtccgatc agccatttaa tagagaaatt tgcgcagcct tacacacatc
    18541 gctaagatcg agccaccgcc tgtaagacga gtaacttacg tgaaacacta ctccatacaa
    18601 cctgccaacc tcgaatttaa tgctgagggt acacctgttt cccgagattt tgacgatgtc
    18661 tatttttcca acgataacgg gctggaagag acgcgttatg tttttctggg aggcaaccaa
    18721 ttagaggtac gctttcctga gcatccacat cctctgtttg tggtagcaga gagcggcttc
    18781 ggcaccggat taaacttcct gacgctatgg caggcatttg atcagtttcg cgaagcgcat
    18841 ccgcaagcgc aattacaacg cttacatttc attagttttg agaaatttcc cctcacccgt
    18901 gcggatttag ccttagcgca tcaacactgg ccggaactgg ctccgtgggc agaacaactt
    18961 caggcgcagt ggccaatgcc cttgcccggt tgccatcgtt tattgctcga tgaaggccgc
    19021 gtgacgctgg atttatggtt tggcgatatt aacgaactga ccagccaaca ggacgattcg
    19081 ctaaatcaaa aagtagatgc ctggtttctg gacggctttg cgccagcgaa aaacccggat
    19141 atgtggacgc aaaatctgtt taacgccatg gcaaggttgg cgcgtccggg cggcacgctg
    19201 gcgacattta cgtctgccgg ttttgtccgc cgcggtttgc aggacgccgg attcacgatg
    19261 caaaaacgta agggctttgg gcgcaaacgg gaaatgcttt gcggggtgat ggaacagaca
    19321 ttaccgctcc cctgctccgc gccgtggttt aaccgcacgg gcagcagcaa acgggaagcg
    19381 gcgattatcg gcggtggtat tgcctgcgcg ttgttgtcgc tggcgctatt acggcgcggc
    19441 tggcaggtaa cgctttattg cgcggatgag gcccccgcac tgggtgcttc cggcaatcgc
    19501 cagggggcgc tgtatccgtt attaagcaaa cacgatgagg cgctaaaccg ctttttctct
    19561 aatgcgttta cttttgctcg tcggttttac gaccaattac ccgttaaatt tgatcatgac
    19621 tggtgcggcg tcacgcagtt aggctgggat gagaaaagcc agcataaaat cgcacagatg
    19681 ttgtcaatgg atttacccgc agaactggct gtagccgttg aggcaaatgc ggttgaacaa
    19741 attacgggcg ttgcgacaaa ttgcagcggc attacttatc cgcaaggtgg ttggctgtgc
    19801 ccagcagaac tgacccgtaa tgtgctggaa ctggcgcaac agcagggttt gcagatttat
    19861 tatcaatatc agttacagaa tttatcccgt aaggatgact gttggttgtt gaattttgca
    19921 ggagatcagc aagcaacaca cagcgtagtg gtactggcga acgggcatca aatcagccga
    19981 ttcagccaaa cgtcgactct cccggtgtat tcggttgccg ggcaggtcag ccatattccg
    20041 acaacgccgg aattggcaga gctgaagcag gtgctgtgct atgacggtta tctcacgcca
    20101 caaaatccgg cgaatcaaca tcattgtatt ggtgccagtt atcatcgcgg cagcgaagat
    20161 acggcgtaca gtgaggacga tcagcagcag aatcgccagc ggttgattga ttgtttcccg
    20221 caggcacagt gggcaaaaga ggttgatgtc agtgataaag aggcgcgctg cggtgtgcgt
    20281 tgtgccaccc gcgatcatct gccaatggta ggcaatgttc ccgattatga ggcaacactc
    20341 gtggaatatg cgtcgttggc ggagcagaaa gatgaggcgg taagcgcgcc ggtttttgac
    20401 gatctcttta tgtttgcggc tttaggttct cgcggtttgt gttctgcccc gctgtgtgcc
    20461 gagattctgg cggcgcagat gagcgacgaa ccgattccga tggatgccag tacgctggcg
    20521 gcgttaaacc cgaatcggtt atgggtgcgg aaattgttga agggtaaagc ggttaaggcg
    20581 gggtaatctg ctctcgcagt gtttgccgga tgcggcgtga acgccttatc cgacctacgt
    20641 gtgacgtgta ggcctgataa gacgcgataa gcgtcgcatc aggcatggtg ctccagacgc
    20701 cgcaacttac tgctgtgacg cctgctgaaa caggttttcc cacatatcgg tcaccagcgc
    20761 ctggtcacgc ggcgacaatt caccggcacc aatggctttt tccagactct ggctaacggt
    20821 cgtatgcacc gcctgagcgg agtggtcatc accactttcc agttctgcga tggctaatgt
    20881 caggtggcca cgcaaatacc cactggcaaa caactcatca tcactggcat ggtcaatcat
    20941 gccgtcgatt aatgccagaa tgcgtgattc aaactccgcg atcatcttct ttcctcattg
    21001 taaaacgtgg caccggcttc agttgagcgc ttccggccac gggaactgtt ccgccgtaag
    21061 ttccggcgtg tgataataat tttgtagtgc tttaatgaag cgtgccggac gctcgggaat
    21121 gccatttgtc agataatcca tcacctgcgc atgtacccgc cgttgaaaca ccacacgatc
    21181 cggttcgaag tcgccttcca gattgtcgca actgacgtta aacggatatc ccgccgccac
    21241 acagaacaac cagtcgagtg cctgcggctt cacttcaaca tcttcaaact ggctttgcgt
    21301 ttgggcatca cgcccatccg ggcaatacca gtagccgaaa tcaaccagct cacggcgcgc
    21361 ttttccggca atacaccagt gcgaaatctc atgaattgcg ctggcataaa agccgtgggc
    21421 aaagacaatt cggttatacg gtacttccgc atcagcagga agatagatcg gttcgtcgtc
    21481 gcctttaatc agacgggtat taaattcatc ggcaaagcag ctattaaaaa tttcaatcaa
    21541 ctgctcatag tggtgtgtac tgttcattag ttcatcccca accagtggag gatctcctgt
    21601 ccgtggctat cgtaaagtaa tttggcactc atcaccgccg agacgataac gatcatcggg
    21661 cggatcagtt tttgtccttt gctcagcacc agtcttgaac ccatgcgcgc gccgaggaac
    21721 tgccccacca gcatgacaaa gcccgtcgcc caaatcactt tgccgccgag aataaacagc
    21781 agcagaccgc caatgtttga cgttgcgttg agtaatttgg cgtgagccgt ggctttggcg
    21841 aggttgaatc cgcacagcgt aacgaaggcc agcgcgtaaa acgatccggc agccgggcca
    21901 aagaatccgt cataaaaacc gacgcagcca ccggcaatta acgcgaaggg taaaccgtac
    21961 atccggcgct ggcgatcttc ttcacccagc tttggcatca gcaagaaata gaggccgata
    22021 caaattacca gaatgggcaa aatctgccgt aagacatcag cctgaacgta ttgcaccagc
    22081 agtgcgccgc tcattgagcc ggcaaaggtc atggcgatat tgagtttctg atcgcttaaa
    22141 ctaaccactt tgcggcgaat aaagtagata gtggcggaaa tagagccgcc gcaggcttgc
    22201 agtttattgg ttgccagcgc gttagcggga gacattcccg cagccatcaa tgccggaatg
    22261 gtgagtaacc caccgccacc ggcaatcgag tcgataaatc ccgccagcat ggcgacaaaa
    22321 aagagaactc ccagcaacag cggggaaacc ataaacaggc tattaaacgt ttccattaga
    22381 tcacgtgctc atccagtagc gcctggcagg aaggcggcaa cggaggcggt gtcttcttct
    22441 caggctttgt tgttcctggt tttggaggtg caaaccagct ttgcagttct gccccgcaac
    22501 catcgcctgg tggcggtaaa ggttgatctt cacactccag actatcggca gggcaacgta
    22561 atcgtacatg catatgcgcg cgatgctgga accagggtcg cactttgcgc aaccagtcgc
    22621 gatcggtgcc cgcatcaagg caaagttgtt gtttaatcgc cggattaaca aaaatgcgcg
    22681 tgacgtcttt gtcctgggcg gcgagtttga tcaagctgaa aatttctggc ttccacagcg
    22741 tggagacaac gtgtttaccg tcgcgggaaa ctaagtccag tgcttgcggg cgcaagagct
    22801 gcgcggaggt ccagcgagtt ttcggcagtt gcagaaagat atcgacatcc agtccggtct
    22861 ggtggctggc atgaccgccg ttgaaacgcc caccagcggg catccccata tcgccaatca
    22921 gcaccgtacc catgcccaga ttgctcacct ggctactcag acgctggata aacatcacca
    22981 gatccgggtg accgaaatag cgacgctgat cggtacgcat gacctgataa tgttcggact
    23041 gtatcggcaa cgtgtcagcg ccgacaatac agccattaga aaaactgcct atcgattgtg
    23101 cgctacccgg cacaggttgg gttatttttt gccacggcgt cgctgccaag ctagcgctac
    23161 tggcaagcag agccagcagc gcaatcgcgg ttttgttcat tttttaccag cgtggaatat
    23221 cagtcttcac atcggcattt tgcgcccgct gccgtaacag gtgatccatt aaaacgatcg
    23281 ccagcatcgc ttcagcgatc ggcactgcac ggatcccgac acagggatcg tgacgacctt
    23341 tggtgatcat ctcaacttct tcgccaaagc ggttaatagt gcgccccggc acggtaatgc
    23401 tggaggtcgg tttcagcgcc atatgagcaa tgatttgctg cccgctgctg ataccgccga
    23461 gaatgccgcc cgcatggttg ctctggaaac cgtctttggt gatttcgtcg cggttctggc
    23521 tgccgcgcag cgccactacg tcaaagccgt cgccaatttc cacgcctttc accgcgttga
    23581 tgctcatcag cgcatgggcg atgtcggcat ccaggcggtc aaataccggc tcgccaagtc
    23641 cggcggggac gccactggca acaacggtga ctttcgcgcc gatggagtcg ccctcttttt
    23701 tcagcgcgcg catcagttca tctaacgcgt cgattttgtc cggatccggg cagaagaacg
    23761 gattttgttc aaccagcgac cagtctttga tttccagcgg aatgtcgccc atctgggtca
    23821 agcagccgcg gatttcaata ccaaatttct cggcgagata ttttttggca atcgcccctg
    23881 ccgccacgcg catggcggtt tcgcgggcgg aagaacgtcc accgccgcga taatcgcgca
    23941 gaccgtattt ttgttcgtag gtgtaatcag catggcctgg gcggaaaacg tctttaattg
    24001 cgctgtaatc ctgagagcgc tggtcagtgt tttcgatcaa caagccaatg ctggtgccag
    24061 tagtaacgcc ttcaaaaaca ccagagagaa ttttgacctg atccggctcg cggcgctggg
    24121 tggtatagcg cgatgtccca gggcgacgac ggtcgaggtc atgttgcagg tccgcttccg
    24181 tcagcagaat gcctggcgga acaccatcga cgatgcagcc gagcgccagc ccatgcgatt
    24241 cgccgaaggt ggttacgcga aagagttgtc caattgtgtt tccagccatc acggctccgt
    24301 tattgttgtg tttgcgtgtt tacttaatct ttataaatcg cgaaatattc acgggccgcc
    24361 agcagctgct ctttggtgag cataaacaca ccatcgccgc cgttatcaaa ctccagccag
    24421 gtgaacggaa catccggata ttgttccata agatgtacca tgctgttgcc gacttcacaa
    24481 atcaacacgc catcatcagc aaggtaatct gccgcgtttc caagaatgcg acgcgtcagt
    24541 ttaaggccgt cggtgccaga tgccaggccc agttccggct cgtgacggta ttcgtttggc
    24601 aggtcggaca tatcttccgc gtcgacatac ggcgggttag tgacaatcag atcgtactgc
    24661 actttcggca agtcgcggaa cagatcggaa cgaatcggaa tgacgttgtg gatcagaccg
    24721 tgttcttcga tgttctgttc agcaaccgcc agcgcgtcgg gagagatatc caccgcgtcg
    24781 acttctgcat ccgggaaggc ataagcacag gcaatggcga tgcagccgct accggtacac
    24841 atatctaaaa tatgctgcgg ttgtttgctg atgagtccgg caaatttatt gttgatcagt
    24901 tcaccaatcg gcgagcgcgg caccagcacg cgttcatcga cgtaaaattc atgaccgcag
    24961 aaccaggctt tgttggtcag gtaagccacc ggaatgcgct cgttgacgcg gcggatcacg
    25021 cgttcaacaa tacgatgttt ttcgctggag gtcagacgcg cggtgcgcat atcttccggg
    25081 atatccagcg gcaggtagag cgatggtaac accagctgta cggcttcatc ccacgggtta
    25141 tcggtgccgt gaccgtacca gatatttgcc gcgctgaagc ggctcactga ccagcgcaac
    25201 atgtcctgaa tggtttgcag ctcatttact gcttcatcaa cgaaaatttt atccacgtat
    25261 tcctccaggg catgatcgca ataatttcgg cggctagttt gccatgaaga tgacgataaa
    25321 tcagcattca cgcgcggtga gtgaggaaaa atacgtttaa aacgatcgat tgtgctacga
    25381 gtcgggtaaa ctgtaggaaa attagaaata gagacagata aatgaaaaag aaaacaacac
    25441 ttagcgagga ggaccaggct ctgtttcgcc agttgatggc ggggactcgc aagattaagc
    25501 aggacacgat tgtccaccga ccgcagcgta aaaaaatcag cgaagtgccg gtgaaacgct
    25561 tgatccagga gcaggctgat gccagccatt atttctccga tgagtttcag ccgttattaa
    25621 ataccgaagg tccggtgaaa tatgttcgcc cggatgtcag ccatttcgag gcgaagaaac
    25681 tgcgccgcgg cgattattca ccggaattgt ttttggattt acacggtctg acgcagctgc
    25741 aggccaagca ggaactgggc gcgttgattg ccgcctgtcg ccgcgaacat gtgttttgtg
    25801 cctgcgtgat gcatgggcac gggaagcata ttttgaagca acaaacaccg ctgtggctgg
    25861 cacaacatcc gcatgtgatg gcatttcacc aggcaccgaa agagtacggc ggtgatgcgg
    25921 ctttgttggt gttgattgaa gtggaagagt ggttgccgcc ggagttgccc tgaggagttg
    25981 agcgtgttgt gccaggagcg cggaatggca ggggcgaatg accctgccag gtgagaaaag
    26041 gtgcgctaat acatattcag tatgtaagtg aatcggcagg tagaatctat gggctgtcga
    26101 gatgtcgtgt ccacgcgtag cttcatatca tgcagttgcc tggacgtaat cggttgcggc
    26161 tcaggcgtca ccgtcacttt ggcattatca cagccggaag tcgagaaggt catctgctgt
    26221 gcgataaaac ggcagtagac accacgctca tcaaaattat tcccgttaca cagtttgcca
    26281 ttttccatca ggaaagtggg cagtgtttgc tcaataacac cacctgttga ctgcaacctg
    26341 acgactttgt tatgtacaac ataattttca ttccaggaaa catcatacgg tgccaggggc
    26401 accgccggac atgaagaact caccttcgtt gggccaatat agtcaggaaa atcatcatca
    26461 tacgttttag cgaagggatc tctactctta acaaacgacg acacaatcca cggacaacca
    26521 tgtaaattcc catcgtaata aacaaacatc tttaattcat tacatgtcca tcccgttttg
    26581 gtctggctat catactgctc taatccagga caggctgaca tggataccct gctgtccgaa
    26641 attttcgaca tagatggttg agtagaacta tcgccagtcc acgaaactaa atccggctgt
    26701 gaattgggca tgaatgtagc cttaaatata gcgtaattaa ctgaatctgg cgtcgctata
    26761 atcagatgaa actcaaactc
//
LOCUS       DAZPEB010000035        25510 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZPEB010000035 DAZPEB010000000
VERSION     DAZPEB010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25510
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.036"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..184
                     /locus_tag="ACHURJ_001320"
     CDS             <1..184
                     /locus_tag="ACHURJ_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015655 - alanine:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0032328 - alanine transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HFR1280339.1"
                     /translation="ACMAITNLTAILLLSPVVHTIASDYLRQRKLGVRPVFDPLRYPD
                     IGRQLSPDAWDDVSQE"
     gene            254..1030
                     /gene="yaaA"
                     /locus_tag="ACHURJ_001321"
     CDS             254..1030
                     /gene="yaaA"
                     /locus_tag="ACHURJ_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFR1280340.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(1183..1479)
                     /locus_tag="ACHURJ_001322"
     CDS             complement(1183..1479)
                     /locus_tag="ACHURJ_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFR1280341.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGNRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(1693..2979)
                     /gene="thrC"
                     /locus_tag="ACHURJ_001323"
     CDS             complement(1693..2979)
                     /gene="thrC"
                     /locus_tag="ACHURJ_001323"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFR1280342.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(2980..3912)
                     /gene="thrB"
                     /locus_tag="ACHURJ_001324"
     CDS             complement(2980..3912)
                     /gene="thrB"
                     /locus_tag="ACHURJ_001324"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFR1280343.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(3914..6376)
                     /gene="thrA"
                     /locus_tag="ACHURJ_001325"
     CDS             complement(3914..6376)
                     /gene="thrA"
                     /locus_tag="ACHURJ_001325"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFR1280344.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTSGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGACRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(6401..6520)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(6456..6521)
                     /gene="thrL"
                     /locus_tag="ACHURJ_001326"
     CDS             complement(6456..6521)
                     /gene="thrL"
                     /locus_tag="ACHURJ_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFR1280345.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(6735..7421)
                     /locus_tag="ACHURJ_001327"
     CDS             complement(6735..7421)
                     /locus_tag="ACHURJ_001327"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFR1280346.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLVESLHDVDFTVATTARSRAKYHYYATPVELVLLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPTKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(7821..7961)
                     /gene="yjjY"
                     /locus_tag="ACHURJ_001328"
     CDS             complement(7821..7961)
                     /gene="yjjY"
                     /locus_tag="ACHURJ_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFR1280347.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            8057..8773
                     /gene="arcA"
                     /locus_tag="ACHURJ_001329"
     CDS             8057..8773
                     /gene="arcA"
                     /locus_tag="ACHURJ_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFR1280348.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(8833..10185)
                     /gene="creD"
                     /locus_tag="ACHURJ_001330"
     CDS             complement(8833..10185)
                     /gene="creD"
                     /locus_tag="ACHURJ_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFR1280349.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMPLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDWATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTMDDQLRIWK
                     "
     gene            complement(10243..11667)
                     /gene="creC"
                     /locus_tag="ACHURJ_001331"
     CDS             complement(10243..11667)
                     /gene="creC"
                     /locus_tag="ACHURJ_001331"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFR1280350.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDSQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPELGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTVVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLEQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVALKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(11667..12356)
                     /gene="creB"
                     /locus_tag="ACHURJ_001332"
     CDS             complement(11667..12356)
                     /gene="creB"
                     /locus_tag="ACHURJ_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFR1280351.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QLVPDVMILDVGLPDISGFELCSQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLHAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(12369..12842)
                     /gene="creA"
                     /locus_tag="ACHURJ_001333"
     CDS             complement(12369..12842)
                     /gene="creA"
                     /locus_tag="ACHURJ_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFR1280352.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            13053..13922
                     /gene="robA"
                     /locus_tag="ACHURJ_001334"
     CDS             13053..13922
                     /gene="robA"
                     /locus_tag="ACHURJ_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFR1280353.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(13919..14566)
                     /gene="gpmB"
                     /locus_tag="ACHURJ_001335"
     CDS             complement(13919..14566)
                     /gene="gpmB"
                     /locus_tag="ACHURJ_001335"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFR1280354.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAARRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            14618..15139
                     /gene="yjjX"
                     /locus_tag="ACHURJ_001336"
     CDS             14618..15139
                     /gene="yjjX"
                     /locus_tag="ACHURJ_001336"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFR1280355.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(15224..15550)
                     /gene="trpR"
                     /locus_tag="ACHURJ_001337"
     CDS             complement(15224..15550)
                     /gene="trpR"
                     /locus_tag="ACHURJ_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFR1280356.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(15640..17577)
                     /gene="sltY"
                     /locus_tag="ACHURJ_001338"
     CDS             complement(15640..17577)
                     /gene="sltY"
                     /locus_tag="ACHURJ_001338"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFR1280357.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            17788..19455
                     /gene="ettA"
                     /locus_tag="ACHURJ_001339"
     CDS             17788..19455
                     /gene="ettA"
                     /locus_tag="ACHURJ_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFR1280358.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(19616..19723)
                     /gene="nadS"
                     /locus_tag="ACHURJ_001340"
                     /pseudo
     CDS             complement(19616..19723)
                     /gene="nadS"
                     /locus_tag="ACHURJ_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
     gene            complement(19762..20994)
                     /gene="nadR"
                     /locus_tag="ACHURJ_001341"
     CDS             complement(19762..20994)
                     /gene="nadR"
                     /locus_tag="ACHURJ_001341"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFR1280359.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(21015..22397)
                     /gene="radA"
                     /locus_tag="ACHURJ_001342"
     CDS             complement(21015..22397)
                     /gene="radA"
                     /locus_tag="ACHURJ_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFR1280360.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(22446..23414)
                     /gene="serB"
                     /locus_tag="ACHURJ_001343"
     CDS             complement(22446..23414)
                     /gene="serB"
                     /locus_tag="ACHURJ_001343"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFR1280361.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            23520..24164
                     /locus_tag="ACHURJ_001344"
     CDS             23520..24164
                     /locus_tag="ACHURJ_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFR1280362.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            24192..25208
                     /gene="lplA"
                     /locus_tag="ACHURJ_001345"
     CDS             24192..25208
                     /gene="lplA"
                     /locus_tag="ACHURJ_001345"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFR1280363.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAEIFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            25240..25503
                     /locus_tag="ACHURJ_001346"
                     /pseudo
     CDS             25240..25503
                     /locus_tag="ACHURJ_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         6131 a         7028 c         6336 g         6015 t
ORIGIN      
        1 ggcctgcatg gctattacca atttgaccgc cattttactg ctctcgcctg tggttcatac
       61 cattgccagt gattatctac gccagcgtaa actcggcgtg cgcccggtgt ttgatccgtt
      121 gcgttatccg gatatcggtc gccagctttc tccggacgcg tgggatgatg tttcgcagga
      181 gtaatcacaa ctatcgatca actcattctc tttttttgct aaagtcggca taaatttcct
      241 gcaaggactg gatatgctga ttcttatttc acctgcgaaa acgcttgatt accaaagccc
      301 gttgaccacc acgcgctata cgctgccgga gctgttagac aattcccagc agttgatcca
      361 tgaagcgcgg aaactgacgc ctccgcagat tagcacgctg atgcgcatca gcgacaaact
      421 ggcgggtatc aacgccgctc gctttcatga ctggcagcca gatttcacgc cggcgaatgc
      481 ccgccaggcg attctggcgt ttaaaggtga tgtctacacc ggcttgcagg ccgaaacctt
      541 cagcgaagac gatttcgatt ttgcccaaca gcatttgcga atgctttccg gcttgtatgg
      601 cgtactgcgc ccgctcgatt taatgcagcc ttatcgtctg gaaatgggga tccgtcttga
      661 gaatgcccga gggaaagatc tgtatcaatt ctggggagat atcatcacca acaagctgaa
      721 cgaggcgctc gcagcacaag gcgataatgt ggtgattaat ctggcgtcag atgagtattt
      781 taaatcggtg aagccgaaga aattgaatgc cgagattatc aagccggtgt tcctcgatga
      841 gaagaacggc aaatttaaga tcatcagctt ctacgctaag aaagcacgcg gtctgatgag
      901 tcgtttcatt attgaaaatc ggctgaccaa accagagcaa ctgactggtt ttaatagcga
      961 aggttacttc tttgatgaag cttcctccag caatggcgaa ctggtgttta aacgctacga
     1021 gcagcgttaa gtttgcagtc aatgccggat gcggcgtgaa cgccttatcc ggcctacaaa
     1081 agcatgcaga ttcaatatat tgcagaattc acgtaggcct gataagacgc gccagcgtcg
     1141 catcaggcgc tgaatgccgg attgttaccc ggcatttgtc atttagcggt gatgtttgcc
     1201 tggaccatga ccgccgtgat gatcatgagg agctttctta tggtggcgcg gcggtggcgg
     1261 cggtccgtgt gggtgccagc gattgcctcg ccattcataa tgttgtttcc accagccgtg
     1321 gtcgcgccag tgacctccat cccagtaata gccacgatta tcacgattgc ctatctgtaa
     1381 ttttactgac gggactaacg taatttccgc agcctgtgct gccatgggag cgaccagaac
     1441 cagggaaagt gcgagtacga tagattgcat ctttttcaca ggttactcct tttatcattg
     1501 cccgttatgg gtcgatatcc ggagattacg cgatgagaac gacggttgtt attctccgca
     1561 atcctaatct ctaagttacc gcatttattg agaatatctc ctttttccct gtcgtttttc
     1621 tccataattt cttcataaaa aagccgggct gcataaaagc aaacccggcc tgattgagat
     1681 aatgaataga ttttactgat gattcatcat caatttacgc aacgcagcaa aatcggcggg
     1741 cagattatgt gaaagcaagg gtaaatcagc acgttctgcc agctcttttg gcagatccaa
     1801 cgtttcaccg agaatcgctt ccacgctctc tttaaatttc gccggatgcg cggtgccgag
     1861 gaacaagcca tattcgcctg gattcaactg gtcacgcagc gcacgatacg ctacggcagc
     1921 gtgcggctcc gaggtgtagc ccagttcttt taactcacgc attgtctgtt gcgtggtttc
     1981 atcatccacg gctgcataac ccagctcttt cagttgccag attttgcggc ggaacaactc
     2041 ttccacacgc ggccagttgt tcggctgact cacgtccatc gcgttggata acgtcgcctg
     2101 agtcgctttg ggtgaccact gaccgtcgtg caggaaacgt ggcacggtat cgttcacgtt
     2161 ggtcgcagca ataaaacgtt tcaccggcag accgagtgac ttcgccagca gacccgccgt
     2221 caaatcgccg aagtttccgc ttggcaccga gacaaccagc tggttgcgcg cttcctgcgg
     2281 cagctgcgca actgcttcaa agtagtagca aatctgcgcc agcaaacggc tgatgttaat
     2341 cgagttagct gagtttaacc ccagcgccac tttcagctct tcatcatcaa acgcctgctt
     2401 caccagcgcc tgacaggcat cgaaatcgcc gtcgatggca acagtttcga tattgccgcc
     2461 caatgtacag aacagttttt cttgcagtgg actgattttg cctcgtggat agaggataac
     2521 cactttcaca ttcggtaaac cgtagaaagc atgagccact gccgctccgg tatcaccgga
     2581 ggtcgcggtc agaatggtca ctggcttatc gcccgcaata tgggtcagca tttgtgccat
     2641 aaagcgaccg ccgaaatctt taaatgccag cgttggcccg tggaacaatt ccagacaacc
     2701 gacatcgctt tcaacattgg cgaccggagc cgggaaggca aacgccgcgc gcacgcgctc
     2761 ttccaggatt tcctgcggga tttcatcacc aataaacgcc gagaggatct tcgcactgcg
     2821 ggtgacaaaa tccagcttca gcatctcatc aatttcagtc aggctgaatt ccggcaggtc
     2881 gtgcggaaaa aacagcccct gatttttgcc caacccctgg gttacggctt gcgcaaagct
     2941 gacctgctca ttgtgatctt tcagattgta gagtttcatt tagttttcca gtactcgtgc
     3001 gcccgccgta tccagccggc aaatatgaac aaaaccttcc tgattttgca ggtagttctt
     3061 acccaaccag tcggcaacgc gctgggcggt atccggcttg tcacacagag cgaacaaggt
     3121 cgggccggag ccggagatac cgctcgctac cgcgccgatt tccgcaaccg cctgccgcgc
     3181 ctgccggaag ccaggcagta accgttcacg gtagggttca gcgataacat ctttcatcag
     3241 cttcgcggca agctcaggct gacgggaata gcaggcgtga atgaagccag ccagatgtcg
     3301 cccgtgcgca atgcaatcct ggcggcgata ctgcgccggt aaaatagccc tggcttctgc
     3361 cgttgagact ttaatccccg gatacgccag cacccacagc cactcatcaa accctggcac
     3421 ttgctggctg atgatgtcgt tttcttcgat catcaactgc ataccaccaa gaaaacacgg
     3481 tgccacgttg tcgtaatgaa tgctgccgga gattcgtcct tccagctcgc ccatcaaagc
     3541 cagcaaacga gtgtcattaa gcggcttgcc acagtgttca ttcatcgcca tcagcgccgc
     3601 gaccaccgaa caggcgctgg agcctaagcc cgaaccgatt ggcatattct tttccagagt
     3661 catcgccact ggaatttgct tgccaagctc ctggcaaaaa cgctcccagc actgataaac
     3721 gatattttcc cgtggttctg acggcagctt atcggcaaag cgtccgaggt tgttgagact
     3781 gaatgtctct gccgcctcaa ccgtgactac atctccgagc aatgcaccat caacaggtgt
     3841 caccgccgcc ccgagcacat caaacccgac gctcatattg gcactggaag ccggggcata
     3901 aactttaacc atgtcagact cctaacttcc atgagagggt acgtagcaga tcggcaaaga
     3961 caccggcagc tgtaacgtca ttgcccgcac catatccgcg cagcaccaac ggcagcggct
     4021 gataatagtg gctataaaag gccagggcgt tttcgccatt tttcactttg aacagcggat
     4081 cattaccatc cacttcggca atcttcacgc ggcaggcacc atcttcatca atattgccaa
     4141 catagcgcaa aacttttcct tcatcacggg ccttcgccac gcgcgcggca aagagatcgt
     4201 cgagctgtga cagattcgcc ataaaagcgg caacatcacc ctcagcgtta aactctgcgg
     4261 gcagcacagg ttcaatttca atatccgcca gctccagttc acgtcccgtt tcacgagcga
     4321 gaatcaatag cttacgcgct acatccatac cagaaagatc atctcgcgga tccggttcgg
     4381 tataacccat ttcccgcgcc agagtagtcg cctcggagaa actcatgcct tcgtctaact
     4441 tgccgaagat ataagaaagc gaacctgaaa gaatgccgga gaacttcatc aattcatcac
     4501 cagcattgag cagattttgc aggttctcaa taaccggtaa tccagcccca acgttggtgt
     4561 catagaggaa tttacgccgc gatttttccg ccgcatgacg caacagatgg tagtaatcca
     4621 tcgacgaggt gttggccttt ttgttcggcg tgacaacgtg gaaaccttcg cgcaagaagt
     4681 cggcatattg atccgccact gcctggctgg aggtgcagtc aacaatgacc gggttcagca
     4741 gatgatattc tttcacgagg cgaattaagc gcccgagatt aaacggctct ttggcttgcg
     4801 ccagttcttc ctgccagttt tccagattta ggccatgcac attggtgagc agtgccttcg
     4861 agttggcaac accgcagaca cgtaagtcga tatgtttatt cttcagccag ctttgttgac
     4921 gcttcagttg ctccagcagc gcaccgccaa cgccaccgac gccaatcaca aacacttcga
     4981 taacctgatc ggtattgaac agcatctgat gagtaacgcg cacgccagtg gtcgcatcat
     5041 cgttatttac cacgacagag attgagcgtt cagaagatcc ctgagcaatg gcgacaatgt
     5101 tgatattggc gcgggccagc gcggcaaaga atttcgccga gatcccacgc aaggtgcgca
     5161 taccatcacc taccaccgag ataatggcca gccgttccgt caccgccagc ggctccagta
     5221 agccttcttt cagttccagg tagaactctt cctgcattgc ccgttcagct cgcacacagt
     5281 cgctttgtgg aacgcagaaa ctgatactgt attcggaaga tgattgcgta atcagcacca
     5341 cggaaatacg ggcgcgtgac attgcagcaa agacgcgcgc cgccatgccg accattcctt
     5401 tcatccccgg accagaaacg ctgaacattg ccatattatt cagattggaa atgcctttga
     5461 ccggtaattc gtcttcatca cggctggcac caatgagcgt acctggtgct tgaggatttc
     5521 cggtattttt aatcaggcaa gggatctgga actgggcgat gggggtaatg gtgcgggggt
     5581 gaagaacttt agcgccgaag taggaaagct ccatcgcttc ctggtaggac atcgacttca
     5641 acaacctcgc atcgggcacc tgacgcgggt cgcaggtata gaccccgtca acgtccgtcc
     5701 aaatctcgca acaatcggcg cgtaaacagg cagccagcac cgccgcggag tagtcggaac
     5761 cgttacgtcc aagtaccacc agttcgcctt tttcattacc ggaggtgaaa cctgccatca
     5821 acaccatgtg atcagccgga atgcggcttg ccgcaatacg gcgggtggac tcagcaatat
     5881 cgacggtaga ttcgaggtaa tgccccactg ccagcagttt ttcgaccgga tcgataacgg
     5941 taacgttgtg accacgagct tctaacacgc cggccataat ggcgatcgac attttctcgc
     6001 cacggcaaat cagcgcagcg ttgatgctat ccgggcactg ccccaacaaa ctaatgccat
     6061 gcaggacatg ttttatttgg gcaaattcct gatcgacgaa agttttcaat tgcgccagcg
     6121 ggaaccccgg ctgggcggcg gcgagtcccg tcaaaagttc ggcaaaaata cgttcggcat
     6181 cgctgatatt gggtaaagca tcctggccgc taatggtttt ttcaatcatc gccaccaggt
     6241 ggttggtgat tttggcgggg gcagagagga cggtggccac ctgcccctgc ctggcattgc
     6301 tttccagaat atcggcaaca cgcagaaaac gttctgcatt tgccactgat gtaccgccga
     6361 acttcaacac tcgcatggtt gttacctcgt tacctttggt cgaaaaaaag cccgcactgt
     6421 caggtgcggg cttttttctg tgtttcctgt acgcgtcagc ccgcaccgtt acctgtggta
     6481 atggtgatgg tggtggtaat ggtggtgcta atgcgtttca tggatgttgt gtactctgta
     6541 atttttatct gtctgtgcgc tatgcctata ttggttaaag tatttagtga cctaagtcaa
     6601 taaaatttta atttactcac ggcaggtaac cagttcagaa actgctatca gacactcttt
     6661 ttttaatcca cacagagaca tattgcccgt tgcagtcaga atgaaaagct gaaaaatact
     6721 tactaaggcg ttttttattt ggtgatattt ttttcaatat catgcagcaa acggtgcaac
     6781 attgccgtgt ctcgttgctc taaaagcccc aggcgttgtt gtaaccagtc gaccagtttt
     6841 atgtcatctg ccactgccag agtcgtcagc aatgccatga ctcgttcgcg taaagcttgc
     6901 agttgatgtt ggtctgccgt tgtgtcactt ttcgtcggtt gttgtattaa tgttgctaat
     6961 tgatagcaat agaccatcac cgcctgcccc agattgagcg aaggataatc cgccaccatc
     7021 ggcacaccag taagaacgtc agccaacgct aactcttcgt tagtcagccc ggaatcttcg
     7081 cgaccaaaca ccagcgcggc atggctcatc catgaagatt tttcctctaa cagcagcacc
     7141 agttctactg gcgtggcgta gtaatgatat ttcgcccgac tgcgcgcagt ggtggcgaca
     7201 gtgaaatcga catcgtgtaa cgattcaacc agtgtcggga aaactttaat attatcaata
     7261 atatcaccag atccatgtgc gacccagcga gtggctggct ccagatgtgc ctgactatcg
     7321 acaatccgca gttcgctaaa acccatcgtt ttcattgccc gcgccgctgc cccaatattt
     7381 tctgctctgg cgggtgcgac cagaataatc gttatacgca tattgccact cttcttgacc
     7441 aaatgaccgc gaacagggtg atcactgtca acttattaca cggtgcgaat ttacaaatcc
     7501 ttaacgtaag tcgcagaaaa agccctttac ttatcttaag taaggctaaa ctatttcctg
     7561 actgtactaa cggttgaatt gttaaaaaat gctacatatc cttctgttta cttaggataa
     7621 ttttataaaa aataaatctc gacaattgga ttcaccacgt ttattagttg tatgatgcaa
     7681 ctagttggat tattaaaata atgtgacgaa agctagcatt tagatacgat gatttcatca
     7741 aactgttaac gtgctacaat tgaacttgat atatgtcaac gaagcgtagt tttattgggt
     7801 gtccggcccc tcttagcctg ttatgttgct gttaaaatgg ttaggatgac agccgttttt
     7861 gacactgtcg ggtcctgagg gaaagtaccc acgaccaagc taatgatgtt gttgacgttg
     7921 atggaaagtg catcaagaac gcaattacgt actttagtca tgttacgccg atcatgttaa
     7981 tttgcagcat gcatcaggca ggtcagggac ttttgtactt cctgtttcga tttagttggc
     8041 aatttaggta gcaaacatgc agaccccgca cattcttatc gttgaagacg agttggtaac
     8101 acgcaacacg ttgaaaagta ttttcgaagc ggaaggctat gatgttttcg aagcgacaga
     8161 tggcgcggaa atgcatcaga tcctctctga atatgacatc aacctggtga tcatggatat
     8221 caatctgccg ggtaagaacg gtcttctgtt agcgcgtgaa ctgcgcgagc aggcgaatgt
     8281 tgcgttgatg ttcctgactg gccgtgacaa cgaagtcgat aaaattctcg gcctcgaaat
     8341 cggtgcagat gactacatca ccaaaccgtt caacccgcgt gaactgacga ttcgtgcacg
     8401 caacctgctg tcccgtacca tgaatctggg tactgtcagc gaagaacgtc gtagcgttga
     8461 aagctacaag ttcaatggtt gggaactgga catcaacagc cgttcgttga tcggccctga
     8521 tggcgagcag tacaagctgc cgcgcagcga gttccgcgcc atgcttcact tctgtgaaaa
     8581 cccaggcaaa attcagtctc gtgctgaact gctgaagaaa atgaccggcc gtgagctgaa
     8641 accacacgac cgtactgtag acgtgacgat ccgccgtatt cgtaaacatt tcgaatctac
     8701 gccggatacg ccggaaatca tcgccaccat ccacggtgaa ggttatcgct tctgtggtga
     8761 tctggaagat taatcggctt taccaccgtc aaaaaaaacg gcgcttttta gcgccgtttt
     8821 tatttttcaa ccttatttcc agatacgtaa ttgatcgtcc attgtaactt ctttactggc
     8881 tttcattttc ggcagtgaaa acgcatacca gtcgatatta cgagtcacaa acatcatgcc
     8941 ggccagcgcc accaccagca cactggttcc caacaacagc gcgctatcgg cagagttgag
     9001 cagtccccac atcacaccat ccagcaacaa cagcgcgagg gtaaacaaca tgctgttgcg
     9061 ccagcctttc aataccgcct gcaaataaat accgttcatt aacgccccaa tcagactggc
     9121 gattatccat gccacggtaa aaccgatatg ttcagaaagc gccagcaaga gcaaataaaa
     9181 catcaccaat gaaagcccca ccagcaaata ttgcattggg tgtaaacgtt gcgcggtgag
     9241 cgtttcaaaa acaaagaacg ccataaaagt cagtgcaatc agcagaatgg cgtacttagt
     9301 cgcccagtcc gttaattggt attgatcggc tggcgtcgtc actgcgacgc taaacgcggg
     9361 gaagttttcc cagccggtat cattgcctga agcaaaacgc tcaccgagat tattagcaaa
     9421 ccagctgctt tgccattgcg cctgaaaacc tgactcgcta acttcccgtt tggctggtag
     9481 aaaatcacct aaaaaactgg gatgcggcca gttgctggtt aagggcattt cgctattacg
     9541 cccggcaggc accacagaaa gatcgccggt accgcttaaa ttcagtgcca tattcagctg
     9601 caggttctgc ttccgccagt ccccttcggg taaagggata tgcacgccct gcccgccttg
     9661 ctctaacccg gtaccgggtt caatggtcag tgccgttccg ttaacttcag gcgctttcac
     9721 cacaccaata ccacgcgcat ccccgacgct aatcacaata aagggcttgc ctaagatgat
     9781 atttggcgcg ttgagctcgc taagacgcga aacatcgaaa tcggctttta gcgttaaatc
     9841 actgtgccag acctgaccgg tataaatccc tatcttgcgt tcttccacgt tctgattgcc
     9901 atcaaccatc aatgactcag gtaaccaaaa atggataaaa cttcgtttcc gctccacggt
     9961 tttatcctct tcctgcaccg tataaagctc ggtcacagga atagcgataa gcggcccaac
    10021 gagtttttgc ggcccgctgg tactttggcg aatcgcatct tccacatcgc tacggtaatc
    10081 ggcacgttcg acaatcactt gccgaatcag cattatcgga atcaacaaca gcaatactgc
    10141 accaaacaag gtggtcattt tccagaacag gggggatttc aacatagtct tctcctttgc
    10201 aatggcagca ggatacgaag gctatgtggg aagaatttga agctatgtga agtgacggtg
    10261 aagtcgaagc gaggccagca cgccaccttc ctgcacgttg cgcagcgtga cttcgccgtt
    10321 aaacaaacgg gcgacctcac tgacgaacgc caaccccaga ccgctgcttt tttgcccatt
    10381 tgcacgcggc aaagagtaaa agcgttcaaa aatacgtgac agcgcgtagt caggaatacc
    10441 actaccggta tccagcacct taagggcgac gtgttcctga tccacttcgg cgcttagcgt
    10501 tatgcgaccg ctctcggggg taaaatcgat ggcgttatcc agtaaattcc ccagcgcctg
    10561 ctccagtaac gccggttcag cagcaacatt aacctcagtg ggcataacat gtagagtgat
    10621 gtttttttct gccaactgca cggtgcgcgc ttcgctgacg cggcgaaata atgccgccac
    10681 atcaacaaca gtcagaacga cttcctgacg attctccagt cttgcctggc gtagtaacgt
    10741 ttctaccaat gcctgcatac gcgcattttg cgtcagaatg ttgtcggtaa aacgagccac
    10801 cacttccggt ggcggacctt cgcgtaaaat ttccgccgcg ccacgaatcg ccgccagtgg
    10861 gctttttagc tcatgggtta acgcataaac atactgctca atatagtttt tcccttccag
    10921 cttcacgcgc atactttcca gcgcctgcgc aagcttacgc aactcgctac tgccgagttc
    10981 aggaagagga acgggcttat tgtcagtgac ggaatcggca tagcgagtga gccgggcaat
    11041 ggagcggttg atccaccaaa ccatgcctgc gccaatcacc agtgcaatcc ccagcaaaat
    11101 ggcgctggcc cataaaattc gccgctcgct acgcttaatg accggagcca tcgccgcgtt
    11161 cggtttgcct acgctcaaaa cgccaataag ccgcgagccg tccataatcg gcgcggcaac
    11221 atacatcacc gaactttcgg gatcggcagg attttgcaac gtgctgcgcg caccatactg
    11281 accacgcaac gttagccaga cgtcattcca gcgcgaataa tcctgcccaa cggctttatt
    11341 tgccgaatcg aacaatactt taccctgcga atcggtcata tagacatgat attcattgcg
    11401 cactttgtta atgccaccga tattggcgcg aaacggacga tgttgtagct gattaaacgc
    11461 ctgcgccagt tgcccatgcg ttgggtcccc agagagcaaa tcgggacgcg ccagctccgc
    11521 cagcaacgtt gcggtgtcga tcaacgtccc ctccgttgct cttcgcacgc ccggtttaac
    11581 ttctttgaca aaaatagcca gtacgaacca ggccgccacc gccaccagta aaaaatagcc
    11641 cagcagcaac cgcatgccga tacgcattac aggcccctca ggctatatcc catgccgcga
    11701 tgagtattaa tcggtgaaag atcggggttg atggcacgca gcttggcatg caacgtttta
    11761 atgtgggtat caacggtgcg atcgtaggtg tcctgcgcat cttcccacac gctatccatc
    11821 agttgctggc gggaccagac gcggcctggc gacttgagta acgtcttcag caataaaaac
    11881 tcataccgcg tcagcgttaa tggcgtgtca aaccagctga tctgcgccgc gggttcattc
    11941 aattcaaaat gaccaatacg gatgacggga gacggcgacg agaacttctt cacccgacgc
    12001 agtaaggtgc gcaccctggc gcacacttcg cggggtgaaa acggtttagc cacatagtcg
    12061 tcagcgccaa tttccagccc aagcaggcga tcgacctctt cacttcgggc cgtcaggaac
    12121 agtacaggta acgccggatg gagcgccagt aactggctgc acaattcaaa gccgctgata
    12181 tcaggcaaac caacgtcgag aatcatgacg tcgggaacca gctggcgagc tttatccagc
    12241 accggcaagc ctcgctcaaa gacctcgacg gcaaaacctt cctgctgcaa catgtagacc
    12301 agcgtgtcgg ctatcccttg ctcatcttcc actaaccaga ccgtttcccg ttgtataaat
    12361 cgcctctgtt attgccgcca cggcatgaca ggaaccgcgc taatcgcgtt tttcggcgaa
    12421 ccttctacaa ctttgtcgga gtaagccaga taagcgagcg cgttgcgttt ggcgtcataa
    12481 aagcgcacga cctgtaacga cttaaagacc agggacgtgc gttttttgaa tactacctcg
    12541 ccctgagctt tgccgttttt aatacgatcc gacagttcaa tcggcccgac ttgctgacaa
    12601 gaaatagccg catcggaggt atcttccgcc agacccaatc cccctttaat accacctgtt
    12661 ttcgcccggc tcacataaca ggtgacattt ttcacatcgg gatcgtcaaa ggcttccaca
    12721 acaattttgt gatccgggcc gatcatttta aatacggtgt cgaccgaacc aatctcttct
    12781 gcatgagcca atggccccag cattatcagg cttaaagaca ggatcaaatg cttgtatttc
    12841 atattgttac catttttatc tattacacga gcaattagtt cgtcacggta aataacatat
    12901 tgattaagaa caaaatctca attcttttat ttccgtcaag ccctaaaaca tactctacta
    12961 agaaaaaaac actgaatgct aaaacagcaa aaaatgctat tatccaatta cctgatgtca
    13021 ggtgctcgtt gttgaaagga tgaggatatt ttatggatca ggccggcatt attcgcgacc
    13081 ttttaatctg gctggaaggt catctggatc agcccctgtc gctcgacaat gtagcggcga
    13141 aagcaggtta ttccaagtgg cacttacaga gaatgtttaa agatgtcact ggccatgcta
    13201 ttggcgcgta tattcgtgct cgtcgtttgt cgaaatcggc ggtcgcacta cgcctgactg
    13261 cgcgtccgat tctggacatc gcgctgcaat accgcttcga ctctcaacag acatttaccc
    13321 gcgcattcaa gaagcagttt gcccagactc ctgcacttta ccgccgttct cctgaatgga
    13381 gcgcctttgg tattcgcccg ccgctacgtc tgggtgaatt cacaatgcca gagcacaaat
    13441 ttgtcaccct ggaagatacg ccgctgattg gtgttaccca gagctactcc tgttcgctgg
    13501 agcaaatctc tgatttccgc catgaaatgc gttatcagtt ctggcacgat tttctcggca
    13561 acgcgccgac cattccgcca gtgctctacg gcctgaatga aacgcgtccg agtcaggata
    13621 aagacgacga acaagaggta ttctatacca ccgcgttagc ccaggatcag gcagatggct
    13681 atgtactgac ggggcatccg gtgatgctgc agggcggcga atatgtgatg tttacctatg
    13741 aaggtctggg aaccggcgtg caggagttta tcctgacggt atacggaacg tgcatgccaa
    13801 tgctcaacct gacgcgccgt aaaggtcagg atattgagcg atactacccg gcagaagacg
    13861 ccaaagcggg agatcgccca attaatctgc gctgtgaact gctgattcct atccgccgtt
    13921 aacgctgcag ctcatctaat gcaggggcgt ccagatgcga gatgtcccct gcggtttcaa
    13981 cgacccagcc agacgccagc cacaggcttt cctgataatc cacgcgcgaa atagaacagt
    14041 tacgcagacg taagcggcgc gctgcccatg ctggtaatcc gagaatcgta ctcaccagac
    14101 atcccagtgc aataccatgg ctcaccagca gcggtcggct tccctgcggt aagtcccggc
    14161 aagattccag tgccgcattg acgcgatcgc tgagctcctg cattgactcg ccttcaggaa
    14221 tacgcccgtc aacggtgcca ttgaccagct gccgacgcca gttctcttct tcttcggtca
    14281 gagaatcgat atgtcttttt tccagcacac ccatgtttaa ttcacgcagg cgagaatcaa
    14341 agatgatgtc acagccgcag gcctgggcga tgatttccgc cgtacgccgg gtgcgtccta
    14401 aatcgctact gataatatgc gtaatgccaa gctctttggc acgggttgcc acctgcatcg
    14461 cctgttgctc acctttggcg gtcagcgggc tgtcagactg gccctgaata cgtcgctcgg
    14521 cgttccactg cgtttcaccg tggcggacta ggtatacctg taacatgctt tttttccgta
    14581 tactgctgta acgttaattt tctgagtaat gctgattatg caccaagttg tctgtgcgac
    14641 caccaatccc gctaaaattc aggccattct gcaggcattt cacgagatct tcggcgaagg
    14701 atcctgccat attgcatccg ttgccgtcga gagcggtgta ccggaacagc cctttggcag
    14761 tgaggaaacg cgcgctggcg cacgaaatcg ggtagcaaat gcccgccgtt tacttccaga
    14821 ggctgatttt tgggtggcga ttgaagctgg catcgatggt gacagcactt tcagttgggt
    14881 ggtgattgaa aacaccagcc agcgcggcga ggcgcgttcg gctactttgc cgttacctgc
    14941 ggtgattctg cagaaagtgc gtgaaggtga ggcgcttggt cccgtgatgt cgcgttatac
    15001 cggtattgat gagattggtc gtaaggaagg ggcgatcggc gtgtttaccg ccgggaaact
    15061 cactcgcgcc agcgtctatc accaggcggt gatcctggcg ctgagtccgt ttcacaatgc
    15121 cgtgtatcag gcattgtagg acggataagg cgttcacgcc gcatccggca ttcggtgcac
    15181 gatgcctgat gcgacgctgc cgcgtcttat caggcctaca aaatcaatcg cttttcagca
    15241 acacctcttc cagccactgg cgcagctcca cgggcgcggc tttcaggctg ttagatccac
    15301 gcgtaatcgt cgcgatgccc gcgccgagtt cattttttaa ctcacgctga ctcatttcgc
    15361 cacgcaacag ctcttcgaca atacgcacgc gagtccccaa cgcttcgcgc tcatctggcg
    15421 tcagcatcag gtttaacaac ggtaaatgga gatcgttttg gtaggcattc ttaagcaggt
    15481 cgacaaaacg taaccactcc tggtgacgct gttctgccat cgctgctgaa tagggtgatt
    15541 gttgggccat aatatgtcgc cattgttagc gggggaagca aaatgcctcc cccggttgta
    15601 ctcgctaaag agtacgatag catatcataa acgtgcggat cagtaacgac gtccccattc
    15661 cgtggcgctc atcaacgtcg gtttatcccc catgaaataa cggtagtaag cgtcataagc
    15721 cagcacgttc ttcacataac cgcgcgtctc ggagaacgga atactctcga caaatgccac
    15781 agcgtcgata cgcccggcgc tgttgccaag ccaggttcgc acccgtccag gtccggcgtt
    15841 ataagctgct gaggagaaaa tacggttatt gccaaactgc tgataaacat attgcaggta
    15901 actggtgcca atgttgatat tcgtttccgg atccagcaat tgcccagggc tgctataacc
    15961 aggaatagag aacatcttca ccgtatgggt cgctgtacca ggcataatct gcatcaggcc
    16021 gctggcccct accggtgatt tcactttcgg attccaggcg ctctcctgac gggcaatcgc
    16081 catcgcatag ctttgcggga tctctttccc actggtgtaa cgtttgaaaa gatcgttgta
    16141 agccagcggg aatcgctctt ccagatgatc ccaaagcttc ccggcgatcg ttgcctgaac
    16201 gctaagatcc caccattggt tattgaaagc ataccgagcc agttgagcct gctctgtttt
    16261 tgacttgctc ttcaccagat tggcccactc gctacgcgcg gtgttatcga gattccagta
    16321 catcaactcg cgcacgcgcg ccatctccgg cccctgagtc agggcgctgt caacattctg
    16381 cggcgcttta tcaatcttca gctcatactc ttctccgatg cgttgtgctg caaccatcgg
    16441 gtagaaacca cgctgttgca tgagttgatg caaaatctct ttcgcttcag cttcacgtcc
    16501 gcgttccagc aataaatccg cctgccagta acgccattca tctttctctt ttgcttccat
    16561 cggcagacgc gccagccagg tattcaggcc gcgacgatcg ccggtgccaa gtgccattcg
    16621 cacacggcgt tcaatcaacg acgtggattg cgagcgcata atggcgtcat cgcgccattt
    16681 cgcctgctcg tcggtgacat cgttgcccat caaacgccag gcgacgatat cgcgcagctc
    16741 ctgaatctga tcttcattaa gctgctgcgc ctgggcaagc gacgggatca tcagccgtgc
    16801 attttcagca tcctgccgcg ccacactggc aaacgccacc gctgccattt gacgggtaaa
    16861 atcggtcgct ccggttgtac gcgcgaaggt cagtaccgta ttagggttgt tcgccagtga
    16921 aatgattgcc gaggcgatag tctggtaatc ggcaggcatc tgccctgcca gcactgttac
    16981 caggccagtg ttacccgctt tcatcgccag acggatacgc tctaaatacg ccagcggatc
    17041 ttgtttacct gacgcacgcc agacgctaaa taacttgtca caggcgttag gctggctctt
    17101 gccggttagc cacagctctt tcgccccttg ccaggcttct tcactctgcc cggtgttcca
    17161 tttcgcatag tagtaattac attgcgcttc ggtagttccg ggcttttccg ggctaaaggc
    17221 taacaagcca cgccagtctt cacgccgcgc cagttcattg acgaaacgag attgcagcgt
    17281 gcgagcggga ggaagcgtgg ggttagcgcg aacaaagtta gtgaccgtca ccgccggttg
    17341 attcatcaga tcatcggtga tctggcggta ttccaggtag ggataaagcg gataatcctt
    17401 cagtccaggc atcatttgtt cgaccacatc catttgtcga ttatcccagg cctgcttgat
    17461 ttgcgcgtaa cggctacgct gctcatccag tgagtcggct cgcgccacgc tgctgaccgt
    17521 cagcagacag acaccggcag ccaacagccg ccaggtaact tgtttggctt tttccacaag
    17581 cgcatcctct aagtgtaaat acatcaatgc agcgtcatgc cgcgtaatag atttatgcta
    17641 accagtcatt gccgtttacg ccacgttacg gacacttttt tacttttact gcgaaggtga
    17701 tcgaccaact gggattaggc ggggaaaaag gctacacttc gcctttgaaa actcatcatc
    17761 acgataaaca tagaggcgaa gtccaacgtg gctcaattcg tttataccat gcatcgtgtc
    17821 ggcaaagttg ttccgccgaa acgtcatatt ttgaaaaaca tctctctgag tttcttccct
    17881 ggggcaaaaa ttggtgtcct gggtctgaac ggcgcgggta agtctaccct gctgcgcatt
    17941 atggcaggca ttgataaaga catcgaaggt gaagcgcgtc cgcagccaga catcaagatt
    18001 ggttacctgc cgcaggaacc gcagctgaac ccggaacaca ccgtgcgtga gtccattgaa
    18061 gaagcggttt ctgaagtggt taacgccctg aaacgcctgg atgaagtgta tgcgctgtac
    18121 gccgatccgg atgccgattt tgacaagctg gccgctgaac aaggccgtct ggaagagatc
    18181 attcaggctc acgacggtca taacctgaac gtacagctgg agcgtgcggc ggatgcgcta
    18241 cgtctgccgg actgggacgc gaaaatcgct aacctctccg gtggtgagcg tcgtcgcgta
    18301 gcgttgtgcc gcctgctgct ggaaaaacca gacatgctgc tgctcgacga accgaccaac
    18361 cacctggatg ccgaatccgt ggcctggctg gaacgcttcc tgcacgactt cgagggcacc
    18421 gtggtggcga ttacccacga ccgttacttc ctcgataacg ttgcaggctg gatcctcgaa
    18481 cttgaccgcg gtgaaggtat tccgtgggaa ggcaactact cctcctggct agagcagaaa
    18541 gatcagcgcc tggcgcagga agcttcacaa gaagcggcgc gtcgtaagtc gatcgagaaa
    18601 gagctggagt gggtacgtca gggaactaaa ggccgccagt cgaaaggtaa agcacgtctg
    18661 gcacgctttg aagagctgaa cagcaccgaa tatcagaaac gtaacgaaac caacgaactg
    18721 tttattccac ctggaccgcg tctgggcgat aaagtgctgg aagtcagcaa cctgcgtaaa
    18781 tcctacggtg atcgactgct gattgatgac ctgagcttct cgatcccgaa aggggcaatc
    18841 gtcgggatca tcggtccgaa cggcgcgggt aaatcgaccc tgttccgtat gatctctggt
    18901 caggaacagc cggacagcgg caccatcact ttaggtgaaa cggtgaaact ggcatcggtt
    18961 gatcagttcc gtgactcaat ggataacagc aaaaccgttt gggaagaagt ttccggcggg
    19021 ctggatatta tgaagatcgg caacaccgag atgccaagcc gcgcctacgt tggccgcttt
    19081 aactttaaag gggttgatca gggtaaacgc gttggtgaac tttccggtgg tgagcgcggt
    19141 cgtctgcatc tggcgaagct gctgcaggtt ggcggcaaca tgctgctgct cgacgaacca
    19201 accaacgacc tggatatcga aaccctgcgc gcgctggaaa acgccctgct ggagttcccg
    19261 ggctgtgcga tggttatctc gcacgaccgt tggttcctcg accgtatcgc cacgcacatc
    19321 ctggactacc aggatgaagg taaagttgag ttcttcgaag gtaactttac tgagtacgaa
    19381 gagtacaaga aacgcacgct gggcgcagac gcactggagc cgaagcgtat caagtacaag
    19441 cgtattgcga agtaatgcgt aaaatgccgg atgcggcgtg aacgccttat ccggcctaca
    19501 aaccaagaaa tttcaatacg ttgtgaatct ccaggcctga taagacgcgg taagcgtcgc
    19561 atcaggcatt gagcaccgaa tgccggatac ggcgtgaacg ccttttccgg cctacaatag
    19621 ccttagtatc cgcaatttcg tagcgggtca cccgtgctga ttcttttaaa ccttgtttaa
    19681 tctcgacagc ctcttccaga gaggttttca actcatcaaa aaacttattt tatacctcgc
    19741 ctttgagccg tttcatcgcg gttatctctg ctcccccatc atctcccgca ccagctccac
    19801 gcagcgcagg aaacggctgt cataatcgtc ctcttcaacc cgcacgaatt cgatattgtt
    19861 ctcttccagc atctccacca gcaagttctg gaactctttg cgatccaccg aactgccgag
    19921 gctgcgtaaa ccatccgcca cccacggcgt gttgttctcc agcaggatca ccagatcgaa
    19981 acggtattca tcaatcaacg cctgtacgaa cggatgctca cgcccttcgt actttttgca
    20041 gaacgcctgg gtagtgacaa aatcagtgtc aataaacgcc actttattgg catatttcac
    20101 tgcaaaatca atgtattgtg cgtggcccag cgcgatttta tcgtaatcgg aatactgcaa
    20161 tgcgatctca tcaccgccga ggtgtgaaaa gacataatcg cgaccatatt cccacgcact
    20221 ggtggtattg aagatattgg caagtttgtt taccagggtg gatttaccgc tcgactcgcc
    20281 accaaggatc gccacggtac gtacaaagaa cggcttcact tccgtaggaa tatattccca
    20341 gtagcggaac gggttttcgc ggatctgcgc accgctgata ctcataaagg tacgtttcgg
    20401 atcgaccagc accgtatcga tccccagatg ttccatatac tgtggcgcat cggcttcttc
    20461 cgaggtgtag atcaggtccg gctgaatccc cttttcagcc ataaactttt tgatgccgtt
    20521 gctccacaca tcccagccgt gcggatacgg ctccatgccc tcctcgttga aagcatgaat
    20581 gcgaatattt ttctgatatt taaaagtttg taataaccag cgcagacgat ccggcaccgt
    20641 gggctgctgc gacatggcgc tgtcttcaaa caacgcgcgg tcgcgggtat cgtcaaaacc
    20701 cataatgata tgcagctcgt caacctggct acaggcgcgc tggataaggt agatatgtcc
    20761 ggtatgcagc gggtagaact taccgaatac gacaccaatg gttttcttct gtcggggaaa
    20821 ttcaagcccc aaaaaacggt gcaacgcctc cagcttttgc gcgctggggc ttttaatttt
    20881 ggcattcagt aactggctta aatacccttt ggtcataccg ctggcatcag ctacctgctg
    20941 tagcgtgcag ccctgttgct tgatggcagt tttcaggtaa tcaaatgacg acatatctcc
    21001 ctccgtatct ctcattataa gtcgtcgaac acgctaagcg cgtcggagag ttttttaacg
    21061 ccaaaaatct gcatcccttc cggcgctttt ttcggtacgt tagccgccgg aacaatcgcc
    21121 cggcgaaaac cgtgtttcgc cgcttctgaa attcgttcct gaccgctggg caccgggcgg
    21181 atctcccctg ccagcccgac ttcaccaaac accaccagat cctgtggcag cggtctgtca
    21241 cgcaggctgg aaaccatcgc cagcagtaac gctaaatcgg cactggtttc ggttaccttc
    21301 acgccgccga ccacgttcac aaatacatcc tgatcggcca tttgcagacc accgtgacgg
    21361 tgcaagacag ccagcaggat tgccagacgg ttttgttcca gccccactgc cacgcggcgc
    21421 ggattcgcca tcatcgagtg atcgaccagc gcctgaatct ccaccagcaa cggacgcgtt
    21481 ccttcccaca ccaccatcac ggagctgccg gaggtcactt catctccgcg acttaagaaa
    21541 attgccgaag ggttgctgac ttcacgcagc ccctgttcgg tcatcgcgaa gacgcccagc
    21601 tcattcaccg cgccgaagcg gtttttatgg ctgcgcaagg tgcgaaaacg ggagtcggca
    21661 tcaccatcca gaagcaccga acagtcgata cagtgttcca gcactttagg gccagccagc
    21721 gagccatctt tggttacgtg cccaaccatg acaatcgcca caccgcgcgt tttggcgaag
    21781 cgcgtcagat aagccgccgt ttcacgcacc tgcgccacgc taccaggcga tgattgtaca
    21841 tccgccatat gcatcacctg gatcgagtca attaccatca gtttcggttg ctcttcttcg
    21901 gcaatcaggc agatctgttc gatgctggtt tccgacaaca tattgagatt gtcagtcggc
    21961 aggccaaggc gatgagcgcg cattgccacc tgttgcagcg actcttcgcc agtgacatac
    22021 agcgttttca tctgctgggc cagtttgcac agcgtttgca gtagcagcgt ggatttcccc
    22081 gcacctgggt taccgccaat cagaatggcg cttcccggca ccacgccgcc gcctagtacg
    22141 cggtcgaact ctttaaatcc ggtggaaaaa cgcggcagct cttcaaggct gatatcggag
    22201 agtttctgga cttttgccac cccggcgcta ccggcatagc cgctgagacg ctcgttacgc
    22261 gccaccgttg gcgacgcagc aagacgcacc tcggtgatgg tgttccaggc atgacaggca
    22321 ctgcactgcc cctgccagcg cggataatcg gccccgcatt cattacaaac aaaggcgcgt
    22381 tttggagctt ttgccacggt gtacctcgtt aatgctgtgc cgcccgcagg atggcgggcg
    22441 agcaattact tctgattcag gctgccagag aggatgcaga atacccccat caggtcagcg
    22501 tgacggatgg tgacttccgc cttttcattc acttttggct tggcatggta ggcaatcccc
    22561 agccctgctg ctttgatcat cggcaggtca ttagctccat cgccaatcgc cacggtctgc
    22621 gccagcggga tttcatactc ctgcgcgagg cgagtcaaag ttttcgcttt gtactgcgcg
    22681 tctacgatgt cgccgatcac attgccggta aatttaccgt ccatgatctc cagttcattg
    22741 gctaccacgg cggtcaggcg cagcttgtcg cgcagatatt cagcaaagaa agtaaagccg
    22801 ccggaggcaa tcgccacttt ccagcccagc gtttccagct tgagcaccag ttgcgttaag
    22861 cctggcatca gcggcagatt ttcacgcacc tgttgcagaa tattggcgtc agcgcctttc
    22921 agcgtcgcca cacggctgcg caggctggcg gtaaaatcga gttcgccgcg catcgcccgt
    22981 tcggttactt ccgccaccat ctcgcccgtt ccggccagtt tggcaatttc atcaatacat
    23041 tcaatctgga tggcggtgga atccatgtcc atcaccagca aacccggcgt gcgcaggtgc
    23101 gggattttcc ccagcggggc gacatccagt tgcgcttcat gggccaggcg cgtagcccgt
    23161 gcggtgagtg aacctgccag acgaatcacc tgataatctt ccacgcacca ggcggcaaca
    23221 atcaccatcg ccgcacccag tttgctctgg tattgggtca gacgctgttt atccagccca
    23281 cgaccataca gcagccagcc gctacgacct gcgtggtaat ccagtggcat cacttcatca
    23341 ccacttaatg aaagaggcag accaggccat aaagagacat cttcaggcag gtcgcaccag
    23401 gtaatgttag gcattaagga tcctgtaaaa tcgttcgaag cagggaaaat aacgcatgag
    23461 gctaccttgt atccattgct tctggcaaca ttaagtctca aattttcaaa gggtggaaga
    23521 tggctcgcac aaaactgaaa ttccggctgc atcgggcagt gattgtcctg ttctgtcttg
    23581 ccttgttagt ggcgctgatg cagggagcgt catggtttag tcaaaaccac cagcgacaac
    23641 gtaatccaca gctggaagaa ctggcccgca ccctggcgcg tcaggtaacg ctgaacgttg
    23701 caccgctgat gcgtaccgac tcaccggatg aaaaacgcat tcaggcgatc ctcgatcagt
    23761 taacggatga aagccgtatc ctcgacgcgg gtgtgtatga cgaacaaggc gatcttatcg
    23821 cacgttctgg cgaaagcgtc gaagtgcgcg accggctggc gctcgacggt aaaaaagcgg
    23881 gcggctattt taaccagcag attgtcgaac caattgccgg gaaaaacgga ccgctcggct
    23941 atctgcgcct gacgctcgac acccatacgc tcgccaccga agcccaacag gtggataaca
    24001 ccactaacat tttacgcctg atgttgctgc tttcactggc aatcggcgta gtgctgaccc
    24061 gcacgctgct acagggtaaa cgtacccgct ggcagcaatc gcccttcctg ttaaccgcca
    24121 gcaaaccggt gccggaagag gaagaaagcg agaaaaaaga gtgacccatt actacaagaa
    24181 aggaaatcgt tatgtccaca ttacgcctgc tcatctctga ctcttacgac ccgtggttta
    24241 acctggcggt ggaggagtgt atttttcgcc agatgcccgc cacgcagcgc gttctgtttc
    24301 tctggcgcaa tgccgacacg gtagtgattg gtcgcgcgca gaacccgtgg aaagagtgta
    24361 atacccggcg gatggaagaa gataacgtcc gcctggcacg gcgcagtagc ggtggcggcg
    24421 cggtgttcca cgatctcggc aatacctgct ttacctttat ggctggcaag ccggagtacg
    24481 ataaaaccat ctccacgtcg attgtgctca atgcgctgaa cgcgcttggc gtcagcgccg
    24541 aagcgtccgg gcgtaacgat ctggtggtga aaaccgccga aggcgaccgc aaagtctcag
    24601 gatcggccta tcgcgaaacc aaagatcgtg gcttccacca cggcaccttg ctgctcaatg
    24661 ccgaccttag ccgcctggca aactatctca atccggataa aaagaaactg gcggcgaaag
    24721 gcattacctc agtgcgttcc cgcgtgacca acctcaccga gctgctgccg gggatcaccc
    24781 atgagcaggt ttgcgaggcc ataaccaagg cctttttcgc ccattatggc gagcgtgtag
    24841 aagcggaaat catctccccg gacaaaacgc cagacttgcc aaacttcgcc gaaatctttg
    24901 cccgtcagag tagctgggaa tggaacttcg gtcaggctcc ggcattctcg catctgctgg
    24961 atgaacgctt tagctggggc ggcgtggaac tgcatttcga cgttgaaaaa ggccatatca
    25021 cccgcgccca ggtgtttacc gacagcctca accccgcgcc gctggaagcc ctcgccgggc
    25081 gactgcaagg ctgcctgtac cgcgcggata tgctgcaaca agagtgcgaa gcgctgttgg
    25141 ttgacttccc ggaccaggaa aaagagctac gggagttgtc gacgtggata gcgggggcgg
    25201 taaggtaact cgctgattaa aataccaaag gaagggaata tgatcgttct ggaaaaccag
    25261 gagcgggaga ttatcaattt aatgttaagc cagcgtattt cctggctagc cgccgtgcgt
    25321 atcaggcata aattatcgct tgctgaagta agtaaaatgt taggtatctc gattaattct
    25381 ctaaaataga tcgaaaagac ggaacggttt agctcaaaca tcaaaagcaa aatggcagaa
    25441 atttatggtt gtccaccaga gttgctgata tgcccttcct ggatgacggc tgaacataaa
    25501 tgaatagatt
//
LOCUS       DAZPEB010000036        25242 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZPEB010000036 DAZPEB010000000
VERSION     DAZPEB010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25242
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.037"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..403)
                     /locus_tag="ACHURJ_001347"
     CDS             complement(<1..403)
                     /locus_tag="ACHURJ_001347"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFR1280364.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGTIVGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLP"
     gene            782..1093
                     /gene="rpsJ"
                     /locus_tag="ACHURJ_001348"
     CDS             782..1093
                     /gene="rpsJ"
                     /locus_tag="ACHURJ_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFR1280365.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            1126..1755
                     /gene="rplC"
                     /locus_tag="ACHURJ_001349"
     CDS             1126..1755
                     /gene="rplC"
                     /locus_tag="ACHURJ_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFR1280366.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            1766..2371
                     /gene="rplD"
                     /locus_tag="ACHURJ_001350"
     CDS             1766..2371
                     /gene="rplD"
                     /locus_tag="ACHURJ_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFR1280367.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            2368..2670
                     /gene="rplW"
                     /locus_tag="ACHURJ_001351"
     CDS             2368..2670
                     /gene="rplW"
                     /locus_tag="ACHURJ_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFR1280368.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            2688..3509
                     /gene="rplB"
                     /locus_tag="ACHURJ_001352"
     CDS             2688..3509
                     /gene="rplB"
                     /locus_tag="ACHURJ_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFR1280369.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            3526..3804
                     /gene="rpsS"
                     /locus_tag="ACHURJ_001353"
     CDS             3526..3804
                     /gene="rpsS"
                     /locus_tag="ACHURJ_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFR1280370.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            3819..4151
                     /gene="rplV"
                     /locus_tag="ACHURJ_001354"
     CDS             3819..4151
                     /gene="rplV"
                     /locus_tag="ACHURJ_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFR1280371.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            4169..4870
                     /gene="rpsC"
                     /locus_tag="ACHURJ_001355"
     CDS             4169..4870
                     /gene="rpsC"
                     /locus_tag="ACHURJ_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFR1280372.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            4883..5293
                     /gene="rplP"
                     /locus_tag="ACHURJ_001356"
     CDS             4883..5293
                     /gene="rplP"
                     /locus_tag="ACHURJ_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFR1280373.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            5293..5484
                     /gene="rpmC"
                     /locus_tag="ACHURJ_001357"
     CDS             5293..5484
                     /gene="rpmC"
                     /locus_tag="ACHURJ_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFR1280374.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            5484..5738
                     /gene="rpsQ"
                     /locus_tag="ACHURJ_001358"
     CDS             5484..5738
                     /gene="rpsQ"
                     /locus_tag="ACHURJ_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFR1280375.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            5903..6274
                     /gene="rplN"
                     /locus_tag="ACHURJ_001359"
     CDS             5903..6274
                     /gene="rplN"
                     /locus_tag="ACHURJ_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFR1280376.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            6285..6599
                     /gene="rplX"
                     /locus_tag="ACHURJ_001360"
     CDS             6285..6599
                     /gene="rplX"
                     /locus_tag="ACHURJ_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFR1280377.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            6614..7153
                     /gene="rplE"
                     /locus_tag="ACHURJ_001361"
     CDS             6614..7153
                     /gene="rplE"
                     /locus_tag="ACHURJ_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFR1280378.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            7168..7473
                     /gene="rpsN"
                     /locus_tag="ACHURJ_001362"
     CDS             7168..7473
                     /gene="rpsN"
                     /locus_tag="ACHURJ_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFR1280379.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            7507..7899
                     /gene="rpsH"
                     /locus_tag="ACHURJ_001363"
     CDS             7507..7899
                     /gene="rpsH"
                     /locus_tag="ACHURJ_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFR1280380.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            7912..8445
                     /gene="rplF"
                     /locus_tag="ACHURJ_001364"
     CDS             7912..8445
                     /gene="rplF"
                     /locus_tag="ACHURJ_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFR1280381.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRIKEAKKK"
     gene            8455..8808
                     /gene="rplR"
                     /locus_tag="ACHURJ_001365"
     CDS             8455..8808
                     /gene="rplR"
                     /locus_tag="ACHURJ_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFR1280382.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            8823..9326
                     /gene="rpsE"
                     /locus_tag="ACHURJ_001366"
     CDS             8823..9326
                     /gene="rpsE"
                     /locus_tag="ACHURJ_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFR1280383.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            9330..9509
                     /gene="rpmD"
                     /locus_tag="ACHURJ_001367"
     CDS             9330..9509
                     /gene="rpmD"
                     /locus_tag="ACHURJ_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFR1280384.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            9513..9947
                     /gene="rplO"
                     /locus_tag="ACHURJ_001368"
     CDS             9513..9947
                     /gene="rplO"
                     /locus_tag="ACHURJ_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFR1280385.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            9955..11286
                     /gene="secY"
                     /locus_tag="ACHURJ_001369"
     CDS             9955..11286
                     /gene="secY"
                     /locus_tag="ACHURJ_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFR1280386.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            11318..11434
                     /gene="rpmJ"
                     /locus_tag="ACHURJ_001370"
     CDS             11318..11434
                     /gene="rpmJ"
                     /locus_tag="ACHURJ_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFR1280387.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            11581..11937
                     /gene="rpsM"
                     /locus_tag="ACHURJ_001371"
     CDS             11581..11937
                     /gene="rpsM"
                     /locus_tag="ACHURJ_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFR1280388.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            11954..12343
                     /gene="rpsK"
                     /locus_tag="ACHURJ_001372"
     CDS             11954..12343
                     /gene="rpsK"
                     /locus_tag="ACHURJ_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFR1280389.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            12377..12997
                     /gene="rpsD"
                     /locus_tag="ACHURJ_001373"
     CDS             12377..12997
                     /gene="rpsD"
                     /locus_tag="ACHURJ_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFR1280390.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            13023..14012
                     /gene="rpoA"
                     /locus_tag="ACHURJ_001374"
     CDS             13023..14012
                     /gene="rpoA"
                     /locus_tag="ACHURJ_001374"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFR1280391.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            14053..14436
                     /gene="rplQ"
                     /locus_tag="ACHURJ_001375"
     CDS             14053..14436
                     /gene="rplQ"
                     /locus_tag="ACHURJ_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFR1280392.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            14543..14911
                     /locus_tag="ACHURJ_001376"
     CDS             14543..14911
                     /locus_tag="ACHURJ_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFR1280393.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILRGIRHDDPQYQEVSRRLSLLELKLRQAGF
                     STDFLRGDYADKLLDKINDN"
     gene            14922..15347
                     /gene="zntR"
                     /locus_tag="ACHURJ_001377"
     CDS             14922..15347
                     /gene="zntR"
                     /locus_tag="ACHURJ_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFR1280394.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            15403..15621
                     /gene="arfA"
                     /locus_tag="ACHURJ_001378"
     CDS             15403..15621
                     /gene="arfA"
                     /locus_tag="ACHURJ_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFR1280395.1"
                     /translation="MSRYQHTKGQIRDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(15618..16028)
                     /gene="mscL"
                     /locus_tag="ACHURJ_001379"
     CDS             complement(15618..16028)
                     /gene="mscL"
                     /locus_tag="ACHURJ_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFR1280396.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(16158..17534)
                     /gene="trkA"
                     /locus_tag="ACHURJ_001380"
     CDS             complement(16158..17534)
                     /gene="trkA"
                     /locus_tag="ACHURJ_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFR1280397.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(17556..18845)
                     /gene="rsmB"
                     /locus_tag="ACHURJ_001381"
     CDS             complement(17556..18845)
                     /gene="rsmB"
                     /locus_tag="ACHURJ_001381"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFR1280398.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAKFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(18891..19838)
                     /gene="fmt"
                     /locus_tag="ACHURJ_001382"
     CDS             complement(18891..19838)
                     /gene="fmt"
                     /locus_tag="ACHURJ_001382"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFR1280399.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERYIRAFNPWPMSWLEIERQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLA"
     gene            complement(19853..20362)
                     /gene="def"
                     /locus_tag="ACHURJ_001383"
     CDS             complement(19853..20362)
                     /gene="def"
                     /locus_tag="ACHURJ_001383"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFR1280400.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            20492..21616
                     /gene="dprA"
                     /locus_tag="ACHURJ_001384"
     CDS             20492..21616
                     /gene="dprA"
                     /locus_tag="ACHURJ_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFR1280401.1"
                     /translation="MVDTDIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     YALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLATSLLEHGGALVSEFPLDVSPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            21588..22061
                     /gene="smg"
                     /locus_tag="ACHURJ_001385"
     CDS             21588..22061
                     /gene="smg"
                     /locus_tag="ACHURJ_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFR1280402.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLTEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLEI
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            22090..22632
                     /locus_tag="ACHURJ_001386"
     CDS             22090..22632
                     /locus_tag="ACHURJ_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFR1280403.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            22637..23209
                     /gene="tsaC"
                     /locus_tag="ACHURJ_001387"
     CDS             22637..23209
                     /gene="tsaC"
                     /locus_tag="ACHURJ_001387"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFR1280404.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVLGETGGRLNPSEIRDALTGELFRQG"
     gene            23214..24032
                     /gene="aroE"
                     /locus_tag="ACHURJ_001388"
     CDS             23214..24032
                     /gene="aroE"
                     /locus_tag="ACHURJ_001388"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFR1280405.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLQGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHLGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            24029..24286
                     /locus_tag="ACHURJ_001389"
     CDS             24029..24286
                     /locus_tag="ACHURJ_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFR1280406.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(24262..24816)
                     /locus_tag="ACHURJ_001390"
     CDS             complement(24262..24816)
                     /locus_tag="ACHURJ_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFR1280407.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGGVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6457 a         5996 c         6569 g         6220 t
ORIGIN      
        1 aaggcagcgg gttttttaat gattcttttc ctctcatgag cagaccaata actacggctc
       61 cgcaagcgaa tgaggcagca aggaaaacca gtcgtggtaa gaaggcccat gaatgccagg
      121 cgccaagggc ggcgagaaac ttcacatcac catagcctaa tccttcttta tgtcgcagta
      181 tgcgataccc ccagtaaatg acagcgaatg tgccgtaacc aacaattgtc ccccataacg
      241 catcggctaa accatcagga tgacaaactt gatagaacaa aagacctgac cagagtaacg
      301 ggcacgtaaa tctgtcaggt aacaagccat gctttgcatc ccagaaaaaa agtagggctg
      361 ataaacaagc gtacaaaatc agaaagggaa gggtagtgtc cataaaacat cctctgtaat
      421 tggagtatca ccatcgaata actccggatg ctcagcaaca gagagagcgc tgatttgtta
      481 acctgattcc ggtgaaaaac gctatcttca caaccttaca aatttcgtgg aatgcagctc
      541 gcaatctgcg cgttgcgatt aatttctacc cgatgcttgc gtcaggctca gatttgcgta
      601 taatgcgcgg gcttgtcgta gttgacagca ggttcaatct gaacctcagt tagccgaatt
      661 tggctaccta acaatgctcc caatcgggga gctacgtaag aacggttaca ctctcccatc
      721 aatcgtaatg ggtctgagga gtaatcattt tcgtttataa aataattgga gctctggtct
      781 catgcagaac caaagaatcc gtatccgcct gaaagcgttt gatcatcgtc tgatcgatca
      841 agcaaccgcg gaaatcgtcg agactgccaa gcgcactggt gcgcaggtcc gtggtccgat
      901 cccgctgccg acacgcaaag agcgcttcac tgttctgatc tccccgcacg tcaacaaaga
      961 cgcgcgcgat cagtacgaaa tccgtactca cttgcgtctg gttgacatcg ttgagccaac
     1021 cgagaaaacc gttgatgctc tgatgcgtct ggatctggct gccggtgtag acgtgcagat
     1081 cagcctgggt taatcaggtc attgagcgat tgagaggttg aaacaatgat tggtttagtc
     1141 ggtaaaaaag tgggtatgac ccgtatcttc acagaagacg gcgtttctat cccagtaacc
     1201 gtaatcgaag ttgaagcaaa ccgcgttact caggttaaag acctggctaa cgatggctac
     1261 cgtgctattc aggtgaccac cggtgctaaa aaagctaacc gtgtgaccaa gcctgaagct
     1321 ggccacttcg ctaaagctgg cgtagaagct ggccgtggtc tgtgggaatt ccgcctggct
     1381 gaaggcgaag agttcactgt aggtcagagc attagcgttg aactgtttgc tgacgttaaa
     1441 aaagttgacg taactggcac ctctaaaggt aaaggtttcg caggtaccgt taagcgctgg
     1501 aacttccgta cccaggacgc tactcacggt aactccttgt ctcaccgcgt tccgggttct
     1561 atcggtcaga accagactcc gggcaaagtg ttcaaaggca agaaaatggc aggtcagatg
     1621 ggtaacgaac gtgtaaccgt tcagagcctt gacgtagtac gcgttgacgc tgagcgcaac
     1681 ctgctgctgg ttaaaggtgc tgtcccgggt gcaaccggta gcgacctgat cgttaaacca
     1741 gctgtgaagg cgtaaggaga tagcaatgga attagtattg aaagacgcgc agagcgcgct
     1801 gactgtttcc gaaactacct tcggtcgtga tttcaacgaa gcgctggttc accaggttgt
     1861 tgttgcttat gcagctggtg ctcgtcaggg tactcgtgct cagaagactc gtgctgaagt
     1921 aactggttcc ggtaaaaaac cgtggcgcca gaaaggcacc ggccgtgcgc gttctggttc
     1981 tatcaagagc ccgatctggc gttctggtgg cgtgaccttt gctgctcgtc cgcaggacca
     2041 cagtcaaaaa gttaacaaga agatgtaccg cggcgcgctg aaaagcatcc tgtccgaact
     2101 ggtacgtcag gatcgtctga tcgttgtcga gaagttctct gtagaagcgc cgaaaactaa
     2161 gctgctggca cagaaactga aagacatggc tctggaagat gtgctgatca tcaccggtga
     2221 gctggacgaa aacctgttcc tggctgcgcg caacctgcac aaggttgacg tacgcgatgc
     2281 aactggtatc gacccggtta gcctgatcgc cttcgacaaa gtcgtaatga ctgctgatgc
     2341 tgttaagcaa gttgaggaga tgctggcatg attcgtgaag aacgtctgct gaaggtgctg
     2401 cgtgcaccgc acgtttctga aaaagcgtct actgcgatgg aaaaatccaa caccatcgta
     2461 ctcaaagttg ctaaagacgc gaccaaagca gaaatcaaag ctgctgtgca gaaactgttt
     2521 gaagtcgaag tcgaagtcgt taacaccctg gtagttaaag ggaaagttaa acgtcacgga
     2581 cagcgtatcg gtcgtcgtag cgactggaaa aaagcttacg tcaccctgaa agaaggccag
     2641 aatctggact tcgttggcgg cgctgagtaa gtcggaggag taatacaatg gcagttgtta
     2701 aatgtaaacc gacatctccg ggtcgtcgcc acgtagttaa agtggttaac cctgagctgc
     2761 acaagggcaa accttttgct ccgttgctgg aaaaaaacag caaatccggt ggtcgtaaca
     2821 acaatggccg tatcaccact cgtcatatcg gtggtggcca caagcaggct taccgtattg
     2881 ttgacttcaa acgcaacaaa gacggtatcc cggcagttgt tgaacgtctt gagtacgatc
     2941 cgaaccgttc cgcgaacatc gcgctggttc tgtacaaaga cggtgaacgc cgttacatcc
     3001 tggcccctaa aggcctgaaa gctggcgacc agattcagtc tggcgttgat gctgcaatca
     3061 aaccaggtaa caccctgccg atgcgcaaca tcccggttgg ttctactgtt cataacgtag
     3121 aaatgaaacc aggtaaaggc ggtcagctgg cacgttccgc tggtacttac gttcagatcg
     3181 ttgctcgtga tggtgcttat gtcaccctgc gtctgcgttc tggtgaaatg cgtaaagtag
     3241 aagcagactg ccgtgcaact ctgggcgaag ttggcaatgc tgagcatatg ctgcgcgttc
     3301 tgggtaaagc aggtgctgca cgctggcgtg gtgttcgtcc gaccgttcgc ggtaccgcga
     3361 tgaacccggt agaccaccca catggtggtg gtgaaggtcg taactttggt aagcacccgg
     3421 taactccgtg gggcgttcag accaaaggta agaagacccg cagcaacaag cgtactgata
     3481 aattcatcgt acgtcgccgt agcaaataat tttagaggat aagccatgcc acgttctctc
     3541 aagaaaggtc cttttattga cctgcacttg ctgaagaagg tagagaaagc ggtggaaagc
     3601 ggagacaaga agcccctgcg cacttggtcc cgtcgttcaa cgatctttcc taacatgatc
     3661 ggtttgacca tcgctgtcca taatggtcgt cagcacgttc cggtatttgt aaccgacgaa
     3721 atggtcggtc acaaactggg tgaattcgca ccgactcgta cttatcgcgg ccacgctgct
     3781 gataaaaaag cgaagaagaa ataaggtagg aggaagagat ggaaactatc gctaaacatc
     3841 gccatgctcg ttcttctgct cagaaggttc gccttgttgc tgacctgatt cgcggtaaga
     3901 aagtgtcgca ggctctggat attttgacct acaccaacaa gaaagcggct gtactggtca
     3961 agaaagttct ggaatctgcc attgctaacg ctgaacacaa cgatggcgct gacattgacg
     4021 atctgaaagt tacgaaaatt ttcgtagacg aaggcccgag catgaagcgc attatgccgc
     4081 gtgcaaaagg tcgtgcagat cgcatcctga agcgcaccag ccacatcact gtggttgtgt
     4141 ccgatcgctg agactctgga gactagcaat gggtcagaaa gtacatccta atggtattcg
     4201 cctgggtatt gtaaaaccat ggaactctac ctggtttgcg aacaccaaag aattcgctga
     4261 caacctggac agcgatttta aagtacgtca gtacctgact aaggaactgg ctaaagcgtc
     4321 cgtatctcgt atcgttatcg agcgtccggc taagagcatc cgtgtaacca ttcacactgc
     4381 tcgcccgggt atcgttatcg gtaaaaaagg tgaagacgta gaaaaactgc gtaaggtcgt
     4441 agcggacatc gctggcgttc ctgcacagat caacatcgcc gaagttcgta agcctgaact
     4501 ggacgcaaaa ctggttgctg acagcatcac ttctcagctg gaacgtcgcg ttatgttccg
     4561 tcgtgctatg aagcgtgctg tacagaacgc aatgcgtctg ggcgctaaag gtattaaagt
     4621 tgaagttagc ggccgtctgg gcggcgcgga aatcgcacgt accgaatggt accgcgaagg
     4681 tcgcgtaccg ctgcacactc tgcgtgctga catcgactac aacacctctg aagcgcacac
     4741 cacttacggt gtaatcggcg ttaaagtgtg gatcttcaaa ggcgagatcc tgggtggtat
     4801 ggctgctgtt gaacaaccgg aaaaaccggc tgctcagcct aaaaagcagc agcgtaaagg
     4861 ccgtaaataa ggagcgtcgc tgatgttaca accaaagcgt acaaaattcc gtaaaatgca
     4921 caaaggccgt aaccgcggtc tggcgcaggg tacggatgtt agcttcggca gcttcggtct
     4981 gaaagctgtt ggccgtggtc gtctgactgc ccgtcagatc gaagcagcac gtcgtgctat
     5041 gacccgtgca gttaagcgtc aaggtaagat ctggatccgt gtgttcccgg acaaaccgat
     5101 cactgaaaag ccgctggcag tgcgtatggg taaaggtaaa ggtaacgtgg agtattgggt
     5161 tgccttgatt cagccgggta aagtcctgta tgaaatggac ggtgttccgg aagagctggc
     5221 ccgtgaagca ttcaagctgg cagcagcgaa actgccgatt aaaaccacct ttgtaactaa
     5281 gacggtgatg taatgaaagc aaaagagctg cgtgagaaga gcgttgaaga gctgaacacc
     5341 gagctgctga acctgctgcg tgagcagttc aacctgcgta tgcaggctgc aagtggccag
     5401 ctgcaacagt ctcacctgtt gaagcaagtg cgtcgcgatg tcgcacgcgt taagacttta
     5461 ctgaacgaga aggctggtgc gtaatgaccg ataaaatccg tactctgcaa ggtcgcgttg
     5521 ttagcgacaa aatggagaaa tccattgttg ttgctatcga acgttttgtg aaacacccga
     5581 tctacggtaa attcatcaag cgtacgacca aactgcacgt acatgacgag aacaacgaat
     5641 gcggtatcgg tgacgtggtt gaaatccgcg aatgccgtcc gctgtccaag actaaatcct
     5701 ggacgctggt tcgcgttgta gagaaagcgg ttctgtaata cagtacactc tctcgatacg
     5761 aataaacggc tcagaaatga gccgtttatt ttttctaccc atatccttga agcggtgtta
     5821 taatgccgcg ccctcgatat ggggattttt aacgacctga ttttcgggtc tcagtagtag
     5881 ttgacattag cggagcacta aaatgatcca agaacagact atgctgaacg tcgccgacaa
     5941 ctccggtgca cgtcgcgtaa tgtgtatcaa ggttctgggt ggctcgcacc gtcgctacgc
     6001 aggcgtaggc gacatcatca agatcaccat caaagaagca attccgcgtg gtaaggtcaa
     6061 aaaaggtgat gtgctgaagg cggtagtggt gcgcaccaag aagggtgttc gtcgcccgga
     6121 cggttctgtc attcgcttcg atggtaatgc ttgtgttctt ctgaacaaca acagcgagca
     6181 gcctatcggt acgcgtattt ttgggccggt aactcgtgag cttcgtagtg agaagttcat
     6241 gaaaattatc tctctggcac cagaagtact ctaaggagcg aatcatggca gcgaaaatcc
     6301 gtcgtgatga cgaagttatc gtgttaaccg gtaaagataa aggtaaacgc ggtaaagtta
     6361 agaatgtcct gtcttccggc aaggtcattg ttgaaggtat caacctggtt aagaaacatc
     6421 agaagccggt tccggccctg aaccaaccgg gtggcatcgt tgaaaaagaa gccgctattc
     6481 aggtttccaa cgtagcaatc ttcaatgcgg caaccggcaa ggctgaccgt gtaggcttta
     6541 gattcgaaga cggcaaaaaa gtccgtttct tcaagtctaa cagcgaaact atcaagtaat
     6601 ttggagtagt acgatggcga aactgcatga ttactacaaa gacgaagtag ttaaaaaact
     6661 catgactgag tttaactaca attctgtcat gcaagtccct cgggtcgaga agatcaccct
     6721 gaacatgggt gttggtgaag cgatcgctga caaaaaactg ctggataacg cagcagcaga
     6781 cctggcagca atctccggtc aaaaaccgct gatcaccaaa gcacgcaaat ctgttgcagg
     6841 cttcaaaatc cgtcagggct atccgatcgg ctgtaaagta actctgcgtg gcgaacgcat
     6901 gtgggagttc tttgagcgcc tgatcactat tgctgtacct cgtatccgtg acttccgtgg
     6961 cctgtccgct aagtctttcg acggtcgtgg taactacagc atgggtgtcc gtgagcagat
     7021 catcttccca gaaatcgact acgataaagt cgaccgcgtt cgtggtttgg atattaccat
     7081 taccactact gcgaaatctg acgaagaagg ccgcgctctg ctggctgcct ttgacttccc
     7141 gttccgcaag taaggtaggg ttactaaatg gctaagcaat caatgaaagc acgcgaagta
     7201 aaacgcgtag ctttagctga taaatacttc gcgaaacgcg ctgaactgaa agcgatcatc
     7261 tctgatgtga acgcttccga cgaagatcgt tggaacgctg ttctcaagct gcagactctg
     7321 ccgcgtgatt ccagcccgtc tcgtcagcgt aaccgctgcc gtcaaacagg tcgtccgcat
     7381 ggtttcctgc ggaagttcgg gttgagccgt attaaggtcc gtgaagccgc tatgcgcggt
     7441 gaaatcccgg gtctgaaaaa ggctagctgg taattgtcac caattgaatc acgggagtaa
     7501 agacagatga gcatgcaaga tccgatcgcg gatatgctga cccgtatccg taacggtcag
     7561 gccgcgaaca aagctgcggt caccatgcct tcctccaagc tgaaagtggc aatcgccaac
     7621 gtgctgaagg aagaaggttt tattgaagat tttaaagttg aaggcgacac caagcctgaa
     7681 ctggaactta ctctgaagta tttccagggc aaagctgttg tagaaagcat tcagcgtgtc
     7741 agccgcccag gtctgcgcat ctataaacgt aaagatgagc tgccgaaagt tatggcgggt
     7801 ctgggtatcg cagttgtttc tacctctaaa ggtgttatga ctgatcgtgc agcgcgccag
     7861 gctggtcttg gtggcgaaat tatctgctac gtagcctaat cggaggaaaa aatgtctcgt
     7921 gttgctaaag caccggtcgt tgttcctgcc ggcgttgacg taaaaatcaa cggtcaggtt
     7981 attacgatca aaggtaaaaa cggcgagctg actcgtactc tcaacgatgc tgttgaagtt
     8041 aaacatgcag ataataccct gaccttcggt ccgcgtgatg gttacgcaga cggttgggca
     8101 caggctggta ccgcgcgtgc cctgctgaac tcaatggtta tcggtgttac cgaaggcttc
     8161 actaagaagc tgcagctggt tggtgtaggt taccgtgcag cggttaaagg taatgtgatt
     8221 aacctgtctc tgggtttctc tcatcctgtt gaccatcagc tgcctgcggg tatcactgct
     8281 gaatgtccga ctcagactga aatcgtgctg aaaggcgctg ataagcaggt gatcggccag
     8341 gttgcagcgg atctgcgcgc ctaccgtcgt cctgagcctt ataaaggcaa gggtgttcgt
     8401 tacgccgacg aagtcgtgcg tatcaaagag gctaagaaga agtaaggtaa cactatggat
     8461 aagaaatctg ctcgtatccg tcgtgcgacc cgcgcacgcc gcaagctcca ggagctgggc
     8521 gcaactcgcc tggtggtaca tcgtaccccg cgtcacattt acgcacaggt aattgcaccg
     8581 aacggttctg aagttctggt agctgcttct actgtagaaa aagctatcgc tgaacaactg
     8641 aagtacaccg gtaacaaaga cgcggctgca gctgtgggta aagctgtcgc tgaacgcgct
     8701 ctggaaaaag gcatcaaaga tgtatccttt gaccgttccg ggttccaata tcatggtcgt
     8761 gtccaggcac tggcagatgc tgcccgtgaa gctggccttc agttctaagg tagaggtgta
     8821 agatggctca catcgaaaaa caagctggcg aactgcagga aaagctgatc gcggtaaacc
     8881 gcgtatctaa aaccgttaaa ggtggtcgta ttttctcctt cacagctctg actgtagttg
     8941 gcgatggtaa cggtcgcgtt ggttttggtt acggtaaagc gcgtgaagtt ccagcagcga
     9001 tccagaaagc gatggaaaaa gcccgtcgca atatgattaa cgtcgcgctg aataacggca
     9061 ctctgcaaca ccctgttaaa ggtgttcaca cgggttctcg cgtattcatg cagccggctt
     9121 ccgaaggtac cggtatcatc gccggtggtg caatgcgcgc cgttctggaa gtcgctgggg
     9181 ttcataacgt tctggctaaa gcatatggtt ccaccaaccc gatcaacgtg gttcgtgcaa
     9241 ctattgatgg cctggaaaat atgaattctc cagaaatggt cgctgccaag cgtggtaaat
     9301 ccgttgaaga aattctgggg aaataaacca tggcaaagac tattaaaatt actcaaaccc
     9361 gcagtgcaat cggtcgtctg ccgaaacaca aggcaacgct gcttggcctg ggtctgcgtc
     9421 gtattggtca caccgtagag cgcgaggata ctcctgctat tcgcggtatg atcaacgcgg
     9481 tttccttcat ggttaaagtt gaggagtaag agatgcgttt aaatactctg tctccggccg
     9541 aaggctccaa aaaggcgggt aaacgcttgg gtcgtggtat cggttctggc ctcggtaaaa
     9601 ccggtggtcg tggtcacaaa ggtcagaagt ctcgttctgg cggtggcgta cgtcgcggtt
     9661 tcgagggtgg tcagatgcct ctgtaccgtc gtctgccgaa attcggcttc acttctcgta
     9721 aagcagcgat tacagccgaa gttcgtctgt ctgacctggc taaagtagaa ggcggtgttg
     9781 tagacctgaa cacgctgaaa gcggctaaca ttatcggtat ccagatcgag ttcgcgaaag
     9841 tgatcctggc tggcgaagta acgactccgg taactgttcg tggcctgcgt gttactaaag
     9901 gcgctcgtgc tgctatcgaa gctgctggcg gtaaaatcga ggaataagta gcagatggct
     9961 aaacaaccgg gattagattt tcaaagtgcc aaaggtggct taggcgagct gaaacgcaga
    10021 ctgctgtttg ttatcggtgc gctgattgtg ttccgtattg gctcttttat tccgatccct
    10081 ggtattgatg ccgctgtact tgccaaactg cttgagcaac agcgaggcac catcattgag
    10141 atgtttaaca tgttctctgg tggtgctctc agccgtgctt ctatctttgc tctggggatc
    10201 atgccgtata tttcggcgtc gatcattatc cagctgctga cggtggttca cccaacgttg
    10261 gcggaaatta agaaagaagg ggagtctggt cgtcgtaaga tcagccagta cacccgctac
    10321 ggtactctgg tgctggcaat attccagtcg atcggtattg ctaccggtct gccgaatatg
    10381 cctggtatgc aaggcctggt gattaacccg ggctttgcat tctacttcac cgctgttgta
    10441 agtctggtca caggaacgat gttcctgatg tggttgggcg aacagatcac tgaacgaggt
    10501 atcggcaacg gtatttcaat cattatcttc gccggtattg tcgcgggact cccgccagcc
    10561 attgcccata ctatcgagca agcgcgtcaa ggcgacctgc acttcctcgt gttgctgttg
    10621 gttgcagtat tagtatttgc agtgacgttc tttgttgtat ttgttgagcg tggtcaacgc
    10681 cgcattgtgg taaactacgc gaaacgtcag caaggtcgtc gtgtctatgc tgcacagagc
    10741 acacatttac cgctgaaagt gaatatggcg ggggtaatcc cggcaatctt cgcttccagt
    10801 attattctgt tcccggcgac catcgcgtca tggttcgggg gcggtactgg ttggaactgg
    10861 ctgacaacaa tttcgctgta tttgcagcct gggcaaccgc tttatgtgtt actctatgcg
    10921 tctgcaatca tcttcttctg tttcttctac acggcgttgg ttttcaaccc gcgtgaaaca
    10981 gcagataacc tgaagaagtc cggtgcattt gtaccaggaa ttcgtccggg agagcaaacg
    11041 gcgaagtata tcgataaagt aatgacccgc ctgaccctgg ttggtgcgct gtatattacc
    11101 tttatctgcc tgatcccgga gttcatgcgt gatgcaatga aagtaccgtt ctacttcggt
    11161 gggacctcac tgcttatcgt tgttgtcgtg attatggact ttatggctca agtgcaaact
    11221 ctgatgatgt ccagtcagta tgagtctgca ttgaagaagg cgaacctgaa aggctacggc
    11281 cgataattgg tcgcccgaga agttacggag agtaaaaatg aaagttcgtg cttccgtcaa
    11341 gaaattatgc cgtaactgca aaatcgttaa gcgtgatggt gtcatccgtg tgatttgcag
    11401 tgccgagccg aagcataaac agcgccaagg ctgatttttt cgcatatttt tcttgcaaag
    11461 ttgggttgag ctggctagat tagccagcca atcttttgta tgtctgtgcg tttccatttg
    11521 agtatcctga aaacgggctt ttcagcatgg aacgtacata ttaaatagta ggagtgcata
    11581 gtggcccgta tagcaggcat taacattcct gatcataagc atgccgtaat cgcattaact
    11641 tcgatttatg gcgtcggcaa gacccgttct aaagccatcc tggctgcagc gggtatcgct
    11701 gaagatgtta agatcagtga gctgtctgaa ggacaaatcg atacgctgcg tgacgaagtt
    11761 gccaaatttg tcgttgaagg tgatctgcgc cgtgaaatca gcatgagcat caagcgcctg
    11821 atggatcttg gttgctatcg cggcttgcgt catcgtcgtg gtctcccggt tcgcggtcag
    11881 cgtaccaaga ccaacgcacg tacccgtaag ggtccgcgca aaccgatcaa gaaataatcg
    11941 gggtgattga ataatggcaa aggcaccaat tcgtgcacgt aaacgtgtaa gaaaacaagt
    12001 ctctgacggc gtggctcata tccatgcttc tttcaacaac accatcgtga ctatcactga
    12061 tcgtcagggt aacgcgttgg gttgggcaac agccggtggt tccggtttcc gtggttctcg
    12121 caaatccact ccgtttgcag ctcaggttgc agcagagcgt tgcgctgacg ccgtgaaaga
    12181 atacggcatc aagaatctgg aagttatggt taaaggtccg ggtccaggcc gcgaatctac
    12241 tattcgtgct ctgaacgccg caggtttccg catcactaac attactgatg tgactccgat
    12301 ccctcataac ggttgtcgtc cgccgaaaaa acgtcgcgta taacgcctcg ttttccaggt
    12361 ttgttggaga aagaaaatgg caagatattt gggtcctaag ctcaagctga gccgtcgtga
    12421 gggcaccgac ttattcctta agtctggcgt tcgcgcgatc gataccaagt gtaaaattga
    12481 acaagctcct ggccagcacg gtgcgcgtaa accgcgtctg tctgactatg gtgtgcagtt
    12541 gcgtgaaaag caaaaagttc gccgtatcta tggtgtgctg gagcgtcagt tccgtaacta
    12601 ctacaaagaa gcagcacgtc tgaaaggcaa caccggtgaa aacctgttgg ctctgctgga
    12661 aggtcgtctg gacaacgttg tataccgtat gggcttcggt gccactcgtg cagaagcacg
    12721 tcagctggtt agccataaag caattatggt aaacggtcgt gttgttaaca tcgcttctta
    12781 tcaggttagt ccgaatgacg ttgtaagcat tcgtgagaaa gcgaagaagc agtctcgcgt
    12841 gaaagccgct ctggagctgg ctgagcagcg tgaaaagcca acttggctgg aagttgatgc
    12901 tggcaagatg gaaggtacgt ttaagcgtaa gccggagcgt tctgatctgt ctgcggacat
    12961 taacgaacac ctgatcgtcg agctttactc caagtaaagc ttagtaccaa agagaggaca
    13021 caatgcaggg ttctgtgaca gagtttctaa aaccgcgcct ggttgatatc gagcaagtga
    13081 gttcgacgca cgccaaggtg acccttgagc ctttagagcg tggctttggc catactctgg
    13141 gtaacgcact gcgccgtatt ctgctctcat cgatgccggg ttgcgcggtg accgaggttg
    13201 agattgatgg tgtactacat gagtacagca ccaaagaagg cgttcaggaa gatatcctgg
    13261 aaatcctgct caacctgaaa gggctggcgg tgagagttca gggcaaagat gaagttattc
    13321 ttaccttgaa taaatctggc attggccctg tgactgcagc cgatatcacc cacgacggtg
    13381 atgtcgaaat cgtcaagccg cagcacgtga tctgccacct gaccgatgag aacgcgtcta
    13441 ttagcatgcg tatcaaagtt cagcgcggtc gtggttatgt gccggcttct acccgaattc
    13501 attcggaaga agatgagcgc ccaatcggcc gtctgctggt cgacgcatgc tacagccctg
    13561 tggagcgtat tgcctacaat gttgaagcag cgcgtgtaga acagcgtacc gacctggaca
    13621 agctggtcat cgaaatggaa accaacggca caatcgatcc tgaagaggcg attcgtcgtg
    13681 cggcaaccat tctggctgaa caactggaag ctttcgttga cttacgtgat gtacgtcagc
    13741 ctgaagtgaa agaagagaaa ccagagttcg atccgatcct gctgcgccct gttgacgatc
    13801 tggaattgac tgtccgctct gctaactgcc ttaaagcaga agctatccac tatatcggtg
    13861 atctggtaca acgtaccgag gttgagctcc ttaaaacgcc taaccttggt aaaaaatctc
    13921 ttactgagat taaagacgtg ctggcttccc gtggactgtc tctgggcatg cgcctggaaa
    13981 actggccacc ggcaagcatc gctgacgagt aaccggatca caggttaagg ttttactgag
    14041 aaggataagg tcatgcgcca tcgtaagagt ggtcgtcaac tgaaccgcaa cagcagccat
    14101 cgccaggcta tgttccgcaa tatggcaggt tcactggttc gtcatgaaat catcaagacg
    14161 actctgccta aagcgaaaga gctgcgccgc gtagttgagc cgctgattac tcttgccaag
    14221 actgatagcg ttgctaatcg tcgtctggca ttcgcccgta ctcgtgataa cgagatcgtg
    14281 gcaaaactgt ttaacgaact gggcccgcgt ttcgcgagcc gtgccggtgg ttacactcgt
    14341 attctgaagt gtggcttccg tgcaggcgac aacgcgccga tggcttacat cgagctggtt
    14401 gatcgttcag agaaagcaga agctgctgca gagtaatctg aagcaacgta aaaaaacccg
    14461 ccccggcggg tttttttata cccgtagcat ccccacttat ctacaatagc tgtactcttt
    14521 ttgttcatcc cctggagtat ttatgtggtt acttgaccag tgggcagagc gtcatatagc
    14581 agaagcgcaa gcgaaaggtg agtttgataa tctggcaggt agcggcgaac cattgatact
    14641 ggatgatgat tctcatgtgc caccggaatt acgtgcgggg tatcgcttgc tgaagaatgc
    14701 tggttgctta ccgccagaac ttgagcagcg gagggaagca attcagcttc tggatatcct
    14761 gagaggtatc cgtcacgatg atccgcaata tcaagaggtt agccgtcgat tgtcattact
    14821 ggaattgaag ctgcgacaag ctggatttag taccgatttt ttacgcggcg attatgctga
    14881 caagttgttg gacaaaatca acgataacta gtggagtatg tatgtatcgc attggtgagc
    14941 tggcaaaaat ggcggaagta acacctgaca cgattcgtta ttacgaaaaa cagcagatga
    15001 tggagcatga agtgcgtact gaaggcgggt ttcgcctgta taccgaaagc gatctccagc
    15061 gattgaaatt tatccgccat gccagacaac taggtttcag tctggagtcg atccgcgagt
    15121 tgctgtcgat ccgcatcgat cctgaacacc atacctgtca ggagtcaaaa ggcattgtgc
    15181 aggaaagatt gcaggaagtc gaagcacgga tagccgagtt gcagagtatg cagcgttcct
    15241 tgcaacgcct taacgatgcc tgttgtggga ccgcccatag cagtgtttat tgttcgattc
    15301 ttgaagctct tgaacaaggg gcgagtggcg ttaagagtgg ctgttgattt tttgcactgg
    15361 caggattaca ctcgcgccgt aaaataacca attggagttt ttatgagccg atatcagcat
    15421 actaaagggc agataaggga taatgcgata gaagcattac tacatgatcc cttattccga
    15481 cagcgcgtag agaaaaataa gaaggggaaa ggcagttaca tgcgaaaagg aaaacatggc
    15541 aatcggggga actgggaggc cagtggcaag aaagtaaatc acttttttac cactggtctt
    15601 ctgctttcag gcgcttgtta agagcggtta ttctgctctt tcagcaaatc acgaatttct
    15661 gccagtaata cttcttcttt agttggtgca ggtgcggcag ctggttcttc ttttttccga
    15721 ttcagtttgt tgattagctt aatcgccata aagatggcaa aggccacaat cagaaaatca
    15781 aagacgtttt gaatgaagac accgtaatgc atcacaacag cagggatatc cccctgcgca
    15841 tcgcgtagcg tgacagcaaa ctgtttaaag tcgatcccgc caattaataa gcccagaggt
    15901 ggcatgatga tatcggcaac cagtgaagat acaatcttcc cgaatgccgc accgataatg
    15961 acacccaccg ccaaatccac cacgttcccg cgcatcgcaa attcgcgaaa ttctttaata
    16021 atgctcatgt tattctccct atgaagccga caaccataag tctaacaaat gttaagccat
    16081 tttcctgcca ggaatgaatt taattaatcc ttgataatca aagggaaata ataaggcgtc
    16141 attagacgcc ttattaatta caagaagaaa gggcttggct ggaagagtct ttcgacgtcg
    16201 gtaataaact ttttatctgt gaggaacata attacgtgat cgccttgctc aatgcgcaga
    16261 ttgtcattgg caatcatcac gtcgtttcca cgtaccaccg ctccaataat cgttcctggc
    16321 ggtagcttga tttcgtcaat gactctgccg acaacgcgtg aggtgctttc atcaccgtga
    16381 gcaacggctt caatagcttc tgctacgccg cggcgcaatg aggaaacacc aacaatatct
    16441 gcttttcgca catggctaag caacgcagaa atagttgctt gttgtggtga aattgcaata
    16501 tcgatcacgc tcccctgaac cagatccaca taagcgcgac gctggatcaa taccatcacc
    16561 tttttcgcac ccatacgttt ggcaagcatg gcggacatga tattggcctc gtcatcgttg
    16621 gtgacggcaa taaacagatc aacttgatcg atatgttctt cggccagcag ttcttgatcc
    16681 gacgcatcac caaaaaagac gatcgtattc tgcaactttt ccgccagttc ggcagcgcgc
    16741 tgctgattac gttcgatgag tttaacgctg taatcttttt ccagacgacg cgccagccct
    16801 gcaccgatat taccgccacc aaccagcatg atccgcttat acggtttttc cagtcgctgt
    16861 aattcactca tcaccgcgcg gatatgctgt gaagcggcaa taaagaacac ttcatcacca
    16921 gcttcaacaa tggtcgaacc ttgtggacga atggggcgat cgtggcggaa aattgctgcc
    16981 acacgagtat cgatatgtgg catatgttcg cgcatggtcg aaagtgcatt accaatcagc
    17041 gggccgccat aataggcttt aactacagcg aggctgactt taccctcagc gaagttcacc
    17101 acctgcaatg cgcctggata ctcaatcagt cggtaaatat tatcgataac caactgctct
    17161 ggtgcgatca gatgatcaat cggtacagca tctgaatgaa atagcttatc ggcatcgcgt
    17221 acgtagtctg gtgagcggat gcgagcgatg cgattagggg tgttgaaaag tgagtaggct
    17281 acctggcagg caaccatatt ggtttcatct gaactggtta cagcaaccag catatcggcg
    17341 tcgtcggcac ctgcctcccg caatacgcgt ggatgagagc catgcccctg cacgactcgc
    17401 aggtcaaatt tatcctgcaa ggtccgcaga cgctcaccgt tggtatcgac aacagtaata
    17461 tcgttgttct cgccaaccag gttttccgcc agtgtgccgc caacctggcc ggcacccaga
    17521 atgataattt tcatcagtct cgacccgtta tctcatcact ttttgattag cttagcgtaa
    17581 aagaagccgt cgccctcttc ggcaccaggc agattttgtt tacccggttg ctctggtgtt
    17641 cctgtttcgc aaagttcggc atcagcggta cgttgcaaaa aagctttaat ctgcaggcta
    17701 ttctcttccg gtaacaccga acaggtggca tagaccaggg ttccaccgga ttttaaatgc
    17761 ggccaaatgg cgtcgagaat ttcagactgc aattgcgcga gttccgggat atcgcgatcg
    17821 cgacgtaacc atttaatatc cggatggcga cgaatcaccc ccgttgctga acaaggcgca
    17881 tctaataaaa tgcgatcaaa ctgttgctcg ccacaccatt gggaagggta acggccatca
    17941 ccttgtttca cggttgcctt cataccaagg cgttttaaat tgtcgtaaac gcgagagagg
    18001 cgctgttcgt caatatcaac cgcaacaacc tgcgcttctg gtgccacctc aaggatatgc
    18061 gttgttttac cgccgggggc ggcacaaaga tccagaatgt gttcaccgtt ttgtggcgca
    18121 agccaggtca tgcaaccttg tgctgatgca tcctgaacgg taacccatcc gtcttcaaaa
    18181 ccaggtagcg catgaacagg tgcaggtgtt tccagacgta cagcatcagg gtaatccgca
    18241 tgcgggaaac ctttcattcc tgcttcatcc agcaatgcaa gccagctgtc gcgggaatga
    18301 tgcgtacgat taacgcgcag ccacattggc ggacgctggt tattggcttc gacgatggat
    18361 tgccactgct ctggatacgc tttttgcaga cgcttcagca accaggaagg atgcagataa
    18421 cgtgcatcac tggcattaaa cttggctaat aactcttctt gctgacgctg gaactggcgt
    18481 aatacaccgt taatcaaccc tttaagttgc ggacgcttaa ttgcgatagc gccttcaacc
    18541 gtttcagcca gcgcagcatg aggtggaatg cgggtataaa gcagttgata caaaccaacc
    18601 ataatcaggt aatgcacagt gcgctgtttg ccggtcatcg gacgggccat taacttatta
    18661 atcagccagt ctaactgcga aagcgtacgc agtacgccaa agcacaactc ttgaagaagt
    18721 gctttgtctt tatcagaaac tttttgctgg agcggtggca gaatgttgct taatgattgc
    18781 ccttgctcga cgacttgttc aacggcctgg gccgccatgc tacgtaaatt acgttgtttt
    18841 ttcataagta taaaaatgcc cggtaagacc gggcttagaa gagtggacta tcaggccaga
    18901 cggttgcccg gaacaaacca ttcccgacga gaattcagga ggtcttgcgc gctcatcgct
    18961 ttcttacccg caggttgtaa cgagagcaga ttcaggattc catcaccagt tgcaacctga
    19021 atgccttgtt tgttggcttc aaggatcgtt cctggtgcag cgttggttgc cgtatcaatg
    19081 accgacgctt tccagacttt aaccggctgt ctttcaattt ccagccagct cattggccat
    19141 ggattgaaag cgcgaatgta gcgttcaagc tgtgctgccg aaagtgacca gtcaatacgc
    19201 gcttcttctt tactcaactt ttcggcgtaa gtgacaagag tttcgtcctg aacttctggt
    19261 ttcgccgtgc cgtctgccag ttgtttcaac gtggtgataa gcccttgtgg gccaagctct
    19321 gccagcttgt cgtacagcgt accactggta tcttctgcag taatcgggca ggagagctta
    19381 tagagcatgt caccggtgtc taaaccgaca tccatttgca taatggtcac accagtttct
    19441 gcatcacccg cccatagtga gcgttggatt ggtgcagcac cgcgccagcg tggcagcagt
    19501 gaaccatgaa cgttgataca gccaagacgc ggcatctcca gcactgcttt aggcagaatt
    19561 aagccatagg cgacgacgac cataacatca gcctgcagat cggcgaccag ttgctggttt
    19621 tcttgtggac gcagggaaac aggttgaaaa acgggcagac ctttttcctc agccagaact
    19681 ttaaccgggc tgggcatcag ttttttaccg cgtcctgccg gtcggtctgg ctgggtgaac
    19741 acgccaacga cgttatgacc agaagacaac agcgcgtcga gatgacgcgc tgcaaagtca
    19801 ggtgtacccg caaaaataat acgtagtgat tctgacacgt tagttcttat ccttaagccc
    19861 gggctttcag acgatccagt ttttcaactt tctgacgaat acgttgttgt ttcaacggtg
    19921 acagataatc cataaacagt ttgccgacca ggtgatccat ctcatgctga atacagatgg
    19981 ctaacagacc gtctgcttcc aattcaaatg gtttaccgtc gcggtccagg gcgcgaattt
    20041 taactttctc tgcgcgtggc actaaagcac gttgttcagg gatcgacagg caaccttctt
    20101 caatgcctgt ttcgccgctt ttttctaaaa gctccggatt gattaacacc agccgttcgt
    20161 cacggttttc cgaaacatca ataacaatga tacgttgatg gatatcaacc tgggttgccg
    20221 ccaggccaat accttcttct gcgtacatcg tctcgaacat atcatcgacg atacgctgaa
    20281 tttctgcatt cacttcttct accggtttag caactttgcg aagccgctcg tccggaatat
    20341 gtaacacttg caaaactgac ataaatctcc agagatgtgt tcaggagtta gaaagattat
    20401 ttcttctatt ctagacaaat ccccctctga ttgacagcat cactgaccaa tcgcaaagat
    20461 tgctaaggct gcttatggca gggagataag gatggtcgat acagatattt ggctgcgttt
    20521 aatgagcatc agcagcttgt acggcgatga tatggtccgt atagctcact ggctggcaaa
    20581 acagtcgcat attgatgcgg ttgtattgca gcaaacaggg cttacattgc ggcaggcaca
    20641 acgctttctt tcatttccgc gaaagagtat cgaaagctca ctttgttggt tggagcaacc
    20701 caaccatcat ttaatccctg cggacagcga attttatcct cctcaacttc tggcgacgac
    20761 agattacccc ggcgcactgt ttgttgaagg agaactgtac gcgctgcatt catttcagct
    20821 tgccgtagtg gggagtcggg cgcattcatg gtatggcgag cgatggggac gattattttg
    20881 cgaaactctg gccacgcgtg gagtgacaat tacgagtgga ctggcgcgtg gaatcgatgg
    20941 tgtggcgcat aaagcggcct tacaggtaaa tggcgtcagc attgctgtat tggggaacgg
    21001 acttaatacc attcatccgc gccgccatgc ccgactggct accagtttgc ttgaacatgg
    21061 tggggcactt gtctcggaat ttcccctcga tgtttcaccc cttgcttaca atttcccacg
    21121 aagaaatcgc attatcagtg gtctaagtaa aggtgtactg gtggtggaag cggctttgcg
    21181 cagtggttcg ttggtgacag cacgttgtgc gcttgagcag gggcgtgaag tttttgcctt
    21241 gccaggacca atagggaatc cgggaagcga agggcctcac tggttaataa aacaaggtgc
    21301 gattcttgtg acggaaccgg aagaaattct ggaaaacttg caatttggat tgcactggtt
    21361 gccagacgcc cctgaaaatt cattttattc accagatcag caagacgtgg cattgccatt
    21421 tcctgagctc ctggctaacg taggagatga ggtaacacct gttgacgtcg tcgctgaacg
    21481 tgccggccaa cctgtgccag aggtagttac tcaactactc gaactggagt tagcaggatg
    21541 gatcgcagct gtacccggcg gctatgtccg attgaggagg gcatgccatg ttcgacgtac
    21601 taatgtattt gtttgaaacc tatattcaca cagaagctga gttgcgtgtg gatcaagaca
    21661 aacttgaaca ggatcttacc gacgcaggat ttgatcgaga agatatctac aatgccctgc
    21721 tttggctgga aaagcttgcg gattatcagg aaggactgac agaaccgatg caactggcct
    21781 ctgatccact ctccatgcgt atttataccc ctgaggagtg tgaaagactg gatgccagct
    21841 gtcgtggttt tctgcttttc cttgagcaaa ttcaggtgct taaccttgaa atccgtgaaa
    21901 tggtgataga gcgagtgctc gcgctggata ccgcagagtt cgacctggaa gacctgaaat
    21961 gggtaatcct gatggtgttg ttcaatattc cgggctgcga aaatgcgtac cagcaaatgg
    22021 aagaattact ctttgaagtg aatgaaggta tgctgcatta atcatttctt taattcagca
    22081 taagttgtta tggcgaaatc agcactgttc acggtgcgta ataatgagtc ctgcccaaag
    22141 tgcggggctg aactggttat tcgatccggg aaacacggtc cgtttcttgg atgctcacag
    22201 tatccggcgt gtgactacgt ccgtcctctg aaatcttcag cggatggaca tattgtcaaa
    22261 gttctggagg ggcaggtttg ccctgcatgt ggcgcaaatc tggtattacg ccagggacgc
    22321 tttggtatgt ttattggttg cagtaactac cctgaatgcg aacataccga acttatcgat
    22381 aaaccggacg aaacagcaat tacatgcccc caatgtcgga cgggccatct ggtccagcgc
    22441 cgctcccgtt atggcaaaac atttcactcc tgtgatcgct acccggagtg tcaatttgcc
    22501 attaacttca aacccatagc tggagaatgc cctgagtgtc attatccact actcatcgaa
    22561 aagaaaaccg cgcagggtgt aaaacacttt tgtgccagta aacaatgtgg aaagccggtt
    22621 tcggcggaat aataacgtga ataataacct gcaaggagac gctatcgcag ctgcgataga
    22681 tgtcctcaat gaagaacgtg tcatcgccta tccaacggaa gccgttttcg gtgttgggtg
    22741 cgatcctgat agcgaaacag cagtgatgag actgttggag ttaaaacagc gtccggttga
    22801 taaggggctg attttaatcg cggcaaatta cgagcagctt aaaccctata ttgatgacac
    22861 catgctgact gatgcgcagc gtgaaaccat tttttcccgc tggccaggtc ctgtcacctt
    22921 tgtctttccc gcgcctgcga caacaccgcg ctggttgacg ggccgctttg attcgcttgc
    22981 tgtacgagtc accgaccatc cgttggtggt tgctttgtgc caggcttatg gtaaaccgct
    23041 ggtttctacc agtgccaact tgagtggatt gccaccttgt cgaacagtag acgaagttcg
    23101 cgcacaattt ggcgcggcgt tcccggttgt gcttggtgaa acgggggggc gtttaaatcc
    23161 ttcagaaatc cgcgatgccc tgacgggtga actgtttcga caggggtaac ataatggaaa
    23221 cctatgctgt ttttggtaat ccgatagccc acagcaaatc gccattcatt catcagcaat
    23281 ttgctcagca actgaatatt gaacatccct atgggcgcgt gttggcaccc atcaatgatt
    23341 tcatcaacac actgaacgct ttctttagtg ctggtggtaa aggtgcgaat gtgacggtac
    23401 cttttaaaga agaggctttt gccagagcgg atgagcttac tgaacgggca gcgttggctg
    23461 gtgctgttaa taccctcaag cggttagaag atggacgctt gcagggtgac aataccgatg
    23521 gtataggttt gttaagcgat ctggaacgtc tgtcttttat ccgtccgggt ttacgtattc
    23581 tgcttatcgg cgctggtgga gcatctcgcg gcgtactact accactcctt tccctggact
    23641 gtgcggtgac aatcactaat cggacggtat cccgtgcgga agagttggct aaattgtttg
    23701 cgcacactgg cagtattcag gcgttgggta tggacgaact ggaaggtcat gagtttgatc
    23761 tcattattaa tgcaacatcc agtggcatca gtggtgatat tccggcgatc ccgtcatcgc
    23821 tcattcatct aggcatttat tgctatgaca tgttctatca gaaaggaaaa actccttttc
    23881 tggcatggtg tgagcagcga ggctcaaagc gtaatgctga tggtttagga atgctggtgg
    23941 cacaggcggc tcatgccttt cttctctggc acggtgttct gcctgacgta gaaccagtta
    24001 taaagcaatt gcaggaggaa ttgtccgcgt gaatcaggcc atccagtttc cggacaggga
    24061 agagtgggac gagaataaaa aatgtgtatg ttttcccgct ctcgtgaatg gtatgcaact
    24121 gacatgcgcg atctctggcg agagtctggc gtatcgcttt actggagata cgccagaaca
    24181 gtggttagcg agttttcgtc agcatcgctg ggacctggaa gaagaagcgg aaaacttaat
    24241 tcaggaacaa agtgaagatg atcaaggctg ggtctggtta ccctgatcca gatattcgtc
    24301 cttccatttc acgtaattat tcgcggaata gcgtaaccca gccttctctt catcacttaa
    24361 cgggcggatc tgtttgacgg ggctaccgag atacagatat ccgctctcca gccgtttatt
    24421 ttgtgggacc agactacccg caccaatcat cacatcatct tctactattg cgccatcaag
    24481 taaaattgag cccatcccaa ccaaaactcg attgccaatg gtgcagccgt ggagcatcac
    24541 cttgtgacca acagtgacat cttcgccaat ggttaatggg ttgccatctg ggttgtacga
    24601 ggatttatga gtgacatgca acatactgcc atcctggata ttggtgcgtg ctccgatctg
    24661 tacataatgt acatctccac gaatcacaac gagcggccag atccccacat catcagccag
    24721 acgaacgcca ccaatcacga cactgctatc gtcgatcatt acgcgctgac cgatttgtgg
    24781 aaaaagatcg cggtatgggc gtaaaacatc agacatactt acctcagcaa taaatgattt
    24841 actaatgact ttgggggcat tattggcctt gtgcaagtct tttagtatgc aaaaaagcac
    24901 cgttttgtgt gcgattgcag caaaaagggt gaaaaaacaa caaacagaaa aaaagatcaa
    24961 aaaaatactt gtgcaaaata ttgggatccc tataatgcgc ctccgttaag acgacaacgt
    25021 gaaacacttc acaggatggt cggaacaacg aagagaaaaa atcctgaaat tcagggttga
    25081 ctctgaaaga ggaaagcgta atatacgcca cctcgcgaca gtgagctgaa agccgcgtcg
    25141 caactgctct ttaacaattt atcagacaat ctgtgtgggc actcgaagat acggattctt
    25201 aacgtcgcaa gacgaaaaat gaataccaag tctcaagagt ga
//
LOCUS       DAZPEB010000037        25118 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZPEB010000037 DAZPEB010000000
VERSION     DAZPEB010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25118
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.038"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..393
                     /gene="hyfA"
                     /locus_tag="ACHURJ_001391"
     CDS             <1..393
                     /gene="hyfA"
                     /locus_tag="ACHURJ_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFR1280408.1"
                     /translation="QLNESLCIGCKLCAVVCPFGAISASGSRPVNAPAQYVFQADGSL
                     KDGEENAPTQHALLRWEPGVQTVAVKCDLCNFLPEGPACVRACPNQALRLITDDSLQR
                     QMKEKQRLAASWFANGGEDPLSLTQEQH"
     gene            393..2410
                     /gene="hyfB"
                     /locus_tag="ACHURJ_001392"
                     /pseudo
     CDS             393..2410
                     /gene="hyfB"
                     /locus_tag="ACHURJ_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            2421..3368
                     /gene="hyfC"
                     /locus_tag="ACHURJ_001393"
     CDS             2421..3368
                     /gene="hyfC"
                     /locus_tag="ACHURJ_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFR1280409.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLLLTPLFTGISRQIRARMHSRRG
                     PGIWQDYREIHKLFKRQEVAPISSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            3385..4614
                     /locus_tag="ACHURJ_001394"
                     /pseudo
     CDS             3385..4614
                     /locus_tag="ACHURJ_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit D"
     gene            4626..5276
                     /gene="hyfE"
                     /locus_tag="ACHURJ_001395"
     CDS             4626..5276
                     /gene="hyfE"
                     /locus_tag="ACHURJ_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFR1280410.1"
                     /translation="MIGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            5281..6861
                     /gene="hyfF"
                     /locus_tag="ACHURJ_001396"
     CDS             5281..6861
                     /gene="hyfF"
                     /locus_tag="ACHURJ_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFR1280411.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVDVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            6851..8566
                     /gene="hyfG"
                     /locus_tag="ACHURJ_001397"
     CDS             6851..8566
                     /gene="hyfG"
                     /locus_tag="ACHURJ_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HFR1280412.1"
                     /translation="MNVNSSSNSGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLHKDAMDYRL
                     RPEPTTDSETYAFINEGNSDARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            8576..9115
                     /gene="hyfH"
                     /locus_tag="ACHURJ_001398"
     CDS             8576..9115
                     /gene="hyfH"
                     /locus_tag="ACHURJ_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFR1280413.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTNKA
                     DLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLRAQASVCPECKQ
                     RATLINDDTDVPLVAKEQL"
     gene            9112..9870
                     /gene="hyfI"
                     /locus_tag="ACHURJ_001399"
     CDS             9112..9870
                     /gene="hyfI"
                     /locus_tag="ACHURJ_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HFR1280414.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKRHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            9863..10276
                     /gene="hyfJ"
                     /locus_tag="ACHURJ_001400"
     CDS             9863..10276
                     /gene="hyfJ"
                     /locus_tag="ACHURJ_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFR1280415.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHRSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTENEDWLAQVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFALLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            10306..10650
                     /locus_tag="ACHURJ_001401"
     CDS             10306..10650
                     /locus_tag="ACHURJ_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase"
                     /protein_id="HFR1280416.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPM
                     PPPFSPVQLIELHVIKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTP"
     gene            10729..12318
                     /locus_tag="ACHURJ_001402"
                     /pseudo
     CDS             10729..12318
                     /locus_tag="ACHURJ_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020454689.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
     gene            12340..13184
                     /gene="focB"
                     /locus_tag="ACHURJ_001403"
                     /pseudo
     CDS             12340..13184
                     /gene="focB"
                     /locus_tag="ACHURJ_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
     gene            complement(13222..14283)
                     /locus_tag="ACHURJ_001404"
     CDS             complement(13222..14283)
                     /locus_tag="ACHURJ_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFR1280417.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTERLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            14496..15959
                     /gene="bepA"
                     /locus_tag="ACHURJ_001405"
     CDS             14496..15959
                     /gene="bepA"
                     /locus_tag="ACHURJ_001405"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFR1280418.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADNADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHLTQRHLARAMEDQQRSTPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            15980..16339
                     /gene="arsC"
                     /locus_tag="ACHURJ_001406"
     CDS             15980..16339
                     /gene="arsC"
                     /locus_tag="ACHURJ_001406"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFR1280419.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(16477..17178)
                     /locus_tag="ACHURJ_001407"
     CDS             complement(16477..17178)
                     /locus_tag="ACHURJ_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFR1280420.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(17273..18562)
                     /gene="uraA"
                     /locus_tag="ACHURJ_001408"
     CDS             complement(17273..18562)
                     /gene="uraA"
                     /locus_tag="ACHURJ_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFR1280421.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEEADITDK"
     gene            complement(18648..19274)
                     /gene="upp"
                     /locus_tag="ACHURJ_001409"
     CDS             complement(18648..19274)
                     /gene="upp"
                     /locus_tag="ACHURJ_001409"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFR1280422.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            19598..20635
                     /gene="purM"
                     /locus_tag="ACHURJ_001410"
     CDS             19598..20635
                     /gene="purM"
                     /locus_tag="ACHURJ_001410"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFR1280423.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            20635..21273
                     /gene="purN"
                     /locus_tag="ACHURJ_001411"
     CDS             20635..21273
                     /gene="purN"
                     /locus_tag="ACHURJ_001411"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFR1280424.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDVTARVQTQEHAIYPLVISWFADGRLKIHENAAWLDGQRLPPQGYAADE"
     gene            21445..23511
                     /gene="ppk1"
                     /locus_tag="ACHURJ_001412"
     CDS             21445..23511
                     /gene="ppk1"
                     /locus_tag="ACHURJ_001412"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFR1280425.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNPEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPHLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            23516..25057
                     /gene="ppx"
                     /locus_tag="ACHURJ_001413"
     CDS             23516..25057
                     /gene="ppx"
                     /locus_tag="ACHURJ_001413"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFR1280426.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRVEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
BASE COUNT         5716 a         6576 c         6684 g         6142 t
ORIGIN      
        1 caactcaacg aaagcctctg tattggctgc aagctttgcg ccgtggtctg cccatttggc
       61 gcaatcagcg cttcaggaag ccgtccggtg aatgcccctg cgcaatatgt ttttcaggct
      121 gacggctcac tcaaagatgg cgaagaaaac gcgccgaccc aacatgcttt gctgcgctgg
      181 gaaccgggtg tccagaccgt cgcggtgaaa tgcgacctgt gtaatttctt gccagaaggt
      241 ccggcctgcg ttcgcgcttg cccgaatcag gcgttacggc tgattaccga cgatagcctg
      301 caacgtcaga tgaaagaaaa acagcgcctt gccgcaagct ggtttgccaa tggcggggag
      361 gatccccttt ccctcactca ggagcaacac taatggatgc cctgcaatta ttaacctggt
      421 cgctgatcct ctttctgttt gccagtctgg cttcgctgtt tttactcggt ctggacagac
      481 tggctattaa gctttccggc atcacatcgc tggtgggcgg cgtgattggc atcatcagcg
      541 gaattacgca attacatgct ggtgtaactt tagtcgcccg ttttgccacc ccttttgact
      601 ttgccgattt aaccctgcga atggatagcc tctcggtatt tatggtgctg gttatctcct
      661 tgctggtggt ggtttgttcg ctctattcat tgacttatat gcgcgaatac gagggcaaag
      721 gcgcggcggc gatgggcttc tttatgaatc ttttcatcgc atcgatggtt gccctgctgg
      781 tgatggacaa cgctttttgg ttcatcgtgc tgtttgaaat gatgtcgctg tcttcctggt
      841 ttctggtcat tgccaggcag gataaaacgt cgatcaacgc tggcatgctc tactttttta
      901 tcgcccacgc cggatcggtg ctgattatga tcgccttctt gctgatgggg cgcgaaagcg
      961 gcagcctcga ttttgccagt ttcgcacgct ttcactttct ccggggctgg cgtcggcgat
     1021 gtttctgctg gcctttttcg gttttggcgc gaaagccggg atgatgccgt tgcacagctg
     1081 gttgccgcgc gctcaccctg ccgcaccatc gcacgcttcg gcgttgatgt ctggcgtaat
     1141 ggtcaaaatc ggtattttcg gcatcctgaa agtagcgatg gatctgctgg cgcaaacggg
     1201 tttgcctctg tggtggggca ttctggtgat ggcgatcggc gcaatctccg cgctcctggg
     1261 cgtgctatat gcgctggcgg aacaggatat caaacggctg ctggcctgga gtaccgtcga
     1321 aaacgtcggc attattttgc tggcagtcgg tgtggcgatg gacggtctgt cactgcacga
     1381 cccgctgctc accgtggttg gactgctcgg cgcactgttt catctgctca accatgcgct
     1441 gttcaaaggg ctgctatttc tcggcgcggg cgcgattatt tcgcgtttgc atacccacga
     1501 catggaaaaa atgggggcac tggcgaaacg gatgccgtgg acagccgcag catgcctgat
     1561 tggttgcctc gcgatatcag ccattcctcc gctgaatggt tttatcagcg aatggtacac
     1621 ctggcagtcg ctgttctcac taagtcgtgt ggaagccgta gcgctacaac ttgcgggtcc
     1681 tattgctatg gtaatgctgg cagtcactgg tgggctggca gtaatgtgct tcgtcaaaat
     1741 gtacggtatt actttctgtg gtgcgccgcg cagtacacac gctgaagagg cacaggaagt
     1801 gccaaatacg atgatcgtcg ccatgctact gctcgcggca ctctgcgtat taattgcgct
     1861 tagtgccagt tggctggcac cgaagataat gcatattgcc catgcgttta ccaatacccc
     1921 tcccgtcact gtcgccagcg gaatagcact tgtacccggc acgtttcata cacgggtcac
     1981 tccttcatta ctgttgctgt tactactggc gatgcctttg ctgcctggcc tttactggct
     2041 gtggtgtcgt tcgcgccgcg cagcgtttcg tcgcacagga gatgcctggg catgcggcta
     2101 cggctgggaa aacgcgatgg ctccgtcagg caatggcgtg atgcagccgc tgcgtgtggt
     2161 cttctctgcg ttatttcgtc tacgacaaca gctcgaccct acgctgaggc taaataaaga
     2221 tcttgcgcac gtcaccgcca gggctcagag cacagaaccc ttctgggatg agcgggtgat
     2281 ccgccccatc gtgagcgcca cccaacggct ggccaaagaa atacagcatc tgcaaagcgg
     2341 cgactttcgt ctctattgcc tgtatctagt tgccgcactg gttgtgctgc taatcgctat
     2401 tgccgtctaa ggaaatcacc atgagacaaa ctctttgcga cggatatctg gtcatttttg
     2461 cgttagcaca ggccgtgatt ctgctgctgc taaccccgct ttttacgggt atttcccggc
     2521 agatacgcgc gcgtatgcac tcccgccgcg ggccggggat ctggcaggat tatcgcgaga
     2581 tccacaaact gtttaaacgc caggaagttg cgcctatatc ttcaggtctg atgttccgcc
     2641 tgatgccgtg ggtattaatc agcagcatgc tggtgctggc gatggcccta ccacttttta
     2701 ttaccgtttc cccttttgcg ggcggcggcg atctgatcac ccttatctat cttcttgccc
     2761 tgtttcgttt tttctttgct ctttccggac tggataccgg aagcccgttt gcgggagtcg
     2821 gtgccagtcg cgagttgacg ctcggcattc tggtcgaacc catgctcatt ctctcactgc
     2881 tggtattggc gctgatagca ggttccacgc atatcgagat gatcagcaat acgctggcaa
     2941 cgggctggaa ctcgccgcta accaccgtac tggcgttact ggcctgtggt tttgcctgct
     3001 tcattgagat gggaaaaatt ccctttgatg ttgctgaagc agaacaggaa ttacaggaag
     3061 gcccgctgac cgaatattcc ggtgccgggc tggcgctagc gaaatggggg ctggggctga
     3121 aacaggtcgt gatggcagca ctgtttgtgg ccctgtttct gccctttggg cgcgcgcaag
     3181 aactttctct cgcctgcctg ctgacttcac ttgtcgttac gctgctcaag gttttgctga
     3241 tttttgtact ggcctcaatc gcagaaaaca cgctggcacg cgggcgtttt ttactcattc
     3301 accatgtgac ctggcttggc ttcagccttg ctgcgcttgc atgggtcttc tggttaaccg
     3361 gtctgtaagg agcactgacg gaatatggaa aatcttgctc tgacgacgtt aatgctgcct
     3421 tttatcggcg cactggtcgt ttcgttttcg ccacaacgtc gggccgccga atgggggttt
     3481 tgttcgccgc actgaccacg ctgtgcatgt tgtcgctgat ctccgcgttt tatcaggccg
     3541 ataaagttgc cgtcacgttg acgttggtca acgtggggga tgtggcattg tttggcctgg
     3601 tcattgatcg cgtgagtacg ctgattctgt ttgtggtggt gtttctcggt ttgctggtca
     3661 cgatctactc cacgggttat ctgacggata aaaatcgcga acacccgcat aacggcacga
     3721 atcgttatta cgcatttttg ctggtgttta tcggcgcgat ggcgggactg gtactctcct
     3781 cgacgctgct cggtcagttg ttgttttttg aaattagggc ggctgctcct gggcgttgat
     3841 cagttattac cagagcgata aagcgcagcg ttcaacacta aaagcgttgc ttatcaccca
     3901 tatcggttcg ttggggttgt atcttgccgc cgccacgctg tttttgcaga ccggaacgtt
     3961 tgtgcttagc gcgatgagcg agttacatgg cgacgcacgt tatctggttt atggcggcat
     4021 cctgtttgcc gcgtggggga aatcggccca gctaccgatg caagcgtggc taccggacgc
     4081 aatggaagcg ccaacaccga tcagagccta tctccacgcc gcatcgatgg tgaaagtggg
     4141 cggctggcac tggagggcag catcgcctac atcgtcaacc acgcgttcgc taaaagcctg
     4201 tttttccttg tagcaggtgc gctgagttac agctgcggca cgcgcttgtt gccgcgtctg
     4261 cgtggcgtat tgcactccct gccgttgcca ggcgtgggtt tctgcgtggc agcgctggcg
     4321 attaccggtg tgccgccgtt caacggcttc ttcagtaaat tcccgctgtt tgccgccggt
     4381 tttgcgttgt cagtggagta ctggatcctg ctgcccgcca tgattctgct gatgattgaa
     4441 tcggtcgcca gtttcgcctg gtttattcgc tggtttggtc gcgttgtgcc tggcaaaccg
     4501 agcgaggccg tcgccgatgc cgcaccgctg ccagggtcaa tgcgcctggt gttgattgta
     4561 ctgattgtga tgtcgctgat ttccagcgta atcgccgcga cctggttgca gtaaggagat
     4621 gatgaatgat cggttctatg atcgtaaata atctggcggg actgatgatg ctgacatcgc
     4681 tgtttgtgat tagcgtcaaa agctatcgcc tgtcatgcgg attttacgcc tgccagtcgc
     4741 tggtgctggt gtctattttc gccactctct cgtgcctgtt cgccgcagag caactgctga
     4801 tctggtccgc cagcgccttt atcaccaaag tgctgctggt accgttaatc atgacttacg
     4861 ctgcacgaaa tattccccag aacatcccgg aaaaagcgtt attcggtccg gcaatgatgg
     4921 cactgctcgc ggcgttaatt gtcctgcttt gcgcatttgt cgttcagccc gtgaagctac
     4981 cgatggctac cgggctgaaa ccggcgctgg cggtagcgtt aggtcatttt ctgcttggcc
     5041 tgctgtgcat tgtcagccag cgcaatatcc tgcggcaaat ttttggttac tgcctgatgg
     5101 aaaacggctc ccatctggtg ctggcgcttc ttgcctggcg agcaccggaa ctggtggaaa
     5161 taggtatcgc taccgacgcc atcttcgccg tcattgtgat ggtgttactg gcaagaaaaa
     5221 tatggcgtac ccacggcacg ctggacgtga acaacttgac cgcgctgaag ggataatgag
     5281 atgagttatt ctgtgatgtt cgctttactc ctgctcacgc cgctgctttt ttcgctgctc
     5341 tgttttgcct gccggaaacg ggggctctct gcgacttgta cggtgactgt attacatagc
     5401 ttagggatca cactgctgct gattctggca ctctgggtgg tccaaactgc cgctgatgca
     5461 ggagaaatat tcgctgcggg actgtggctt catattgatg gtctgggcgg tttgttcctc
     5521 gccattcttg gtgtgattgg ctttctcacc ggtgtttact cgattggcta catgcgtcat
     5581 gaagtggcac acggcgagct ttcacccgtt acactgtgcg attactacgg tttcttccat
     5641 ctgtttttgt tcaccatgct gctggttgtt accagcaata acctgattgt gatgtgggcg
     5701 gcgatcgaag ccaccacctt aagctcggcg tttctggtag gcatttacgg tcagcgttca
     5761 tcgctggaat cagcatggaa gtacatcatt atttgtactg ttgatgtcgc ttttggtctg
     5821 ttcggtaccg tgctggtata cgccaacgcc gccagcgtta tgccgcaggc agaaatggcg
     5881 atattctgga gcgaggttct taagcaatcg tccttgcttg acccaacatt aatgctgttg
     5941 gcctttgtgt ttgtgctaat tggctttggt accaaaaccg ggctatttcc catgcacgcc
     6001 tggctgccgg atgctcacag tgaagcgccg agtccggtca gcgccctgct ctccgccgta
     6061 ttgctgaact gcgcgctgtt ggtgctgatt cgctattaca tcattatttg ccaagccatc
     6121 ggcagcgatt tccccaaccg gttgttgctc atcttcggca tgttgtcggt tgccgtggcg
     6181 gcatttttca ttctggtaca gcgggacatt aagcgtctgc tggcgtactc cagcgtggag
     6241 aacatggggc tggtcgcggt ggcgctaggc attggcgggc cgctgggaat ttttgccgcg
     6301 ctgctgcaca ccttaaacca cagtctggca aaaacgctgc tgttctgcgg ttccggcaat
     6361 gtactgctca agtacggcac gcgcgatctc aacgtcgtct gtgggatgct caaaatcatg
     6421 ccatttaccg ccgtgctgtt tggcggcggt gcgctggcgc tggcagggat gccgcccttc
     6481 aacatttttc ttagcgaatt tatgaccgtt accgcaggac tggcacgtaa tcacctgctg
     6541 attatcgtcc tgctgttatt gctgttaacg ctggtgctgg cgggcctggt acggatggct
     6601 gcgcgggtgt taatggcgaa accgccgcag gccgttaacc ggggtgatct cggctggttg
     6661 accacctcgc caatggtgat tctgctggtc atgatgctgg cgatgggaac gcatattcca
     6721 caacctgtca tcaggatcct ggcgggcgct tccactatag tcctctcagg gacgcacgac
     6781 ctgcctgcac aacgtagcac ctggcatgat tttttgcctt caggcaccgc atctgtttcg
     6841 gagaaacaca gtgaacgtta attcatcgtc aaatagtggc gaagcgattc tcgccgccct
     6901 gaaaacgcag ttccccggcg cggtactgga tgaagagcga caaacgcctg aacaggtcac
     6961 cattacggtg aaaatcaatc tgctgcctga cgttgtacat tatctttatt atcaacatga
     7021 tggctggctt ccagtcctgt ttggcaacga cgagcggaca cttaacggtc attacgcggt
     7081 ttattatgcc ctttcaatgg aaggggccga aaaatgctgg atcgtggtga aggcactggt
     7141 cgatgccgac agtcgggagt ttccgtcagt cacaccgcgc gtccctgccg cggtctgggg
     7201 cgagcgagaa attcgcgata tgtacgggct gattccggtt ggcctgccgg atcagcgtcg
     7261 cctggtgttg cccgatgact ggccggaaga tatgcatccg ctgcacaaag atgcgatgga
     7321 ttatcgactg cgccctgaac cgacgacaga ttccgaaacg tatgcgttta tcaatgaggg
     7381 caacagcgat gcgcgggtga tccctgtcgg cccgctgcat atcacctccg atgaaccggg
     7441 tcacttccgc ttgtttgtgg atggcgagca aattgtcgat gctgattacc gcctgtttta
     7501 tgtccatcga ggcatggaga aactggcaga aacgcggatg ggctacaacg aagtgacctt
     7561 cttatcggac cgcgtgtgtg ggatttgcgg ttttgcccac agtgtggcct ataccaactc
     7621 ggttgaaaat gcactgggga ttgaggtgcc gcaacgagcg cataccattc gctcgattct
     7681 gctggaagtc gaacggctac acagtcattt gctcaacctt ggcctctcct gccattttgt
     7741 tggttttgat accggcttta tgcaattttt ccgcgtgcgg gaaaagtcga tgacgatggc
     7801 ggaattgctg accgggtcgc gtaaaaccta cggtctgaat ctgattggtg gtgttcgccg
     7861 cgatattctc aaagagcaac gtctgcaaac gctgaaactg gtgcgcgaga tgcgcgccga
     7921 cgtgtcggag ctggtagaga tgctgcttgc tacgccgaat atggaacaac gcactcaggg
     7981 cattggcatt ctcgaccgac aaatcgcccg tgattatagc cctgtagggc cgctgatccg
     8041 cggcagtggt tttgcccgtg atttgcgctt tgatcacccc tacgccgact acggcaatat
     8101 tccaaaaaca ctgtttacct tcaccggcgg cgatgttttc tcccgcgtga tggtccgtgt
     8161 caaagagacg tttgattcgc tggcaatgct ggaatttgcc ctcgacaaca tgccggatac
     8221 cccactgctg accgaaggct ttagctataa acctcacgca ttcgcgctgg gctttgttga
     8281 agcgccacgc ggtgaagacg tgcactggag catgctcggt gataaccaaa aattgttccg
     8341 ctggcgctgc cgtgccgcca cctacgccaa ctggccggtg ttgcgttaca tgctgcgcgg
     8401 caataccgtt tctgacgcac cgctgattat cggtagcctt gatccctgct actcctgtac
     8461 cgaccgtgtg acgctggtag atgtgcgcaa gcgccagtca aaaaccgtgc cgtataaaga
     8521 gatcgaacgc tacggcattg atcgtaaccg ttcgccgctg aagtaaggac agaagatgct
     8581 gaagttactg aaaactatta tgcgcgccgg aaccgcgacg gtgaaatatc ccttcgcgcc
     8641 actggaggtc agcccaggct ttcgcgggaa accggacctg atgcccagcc aatgtattgc
     8701 ctgcggcgcc tgtgcttgtc cggcaaatgc gctgactatc cagaccgacg accagcaaaa
     8761 ttcgcgcacc tggcagctct atctggggcg ttgtatttac tgcggacgtt gtgaagaagt
     8821 gtgcccgacc agagccatcc agcttaccaa taactttgaa ctgaccgtca ccaataaagc
     8881 cgatctctat acccgcgcga cgttccatct acaacgttgc agccgttgcg aacgcccgtt
     8941 tgccccgcaa aaaaccgtcg cactggctgc tgaattgttg gcacagcaac aaaatgcgcc
     9001 acaaaaccgc gaaatgttgc gggcgcaagc gagcgtctgc ccggagtgca aacaacgcgc
     9061 gacgctgatc aacgacgata cagatgtacc gctggtggct aaggagcagc tatgagtcca
     9121 gtgcttacac aacatgtcag ccagcccatc acgctggatg agcaaacgca aaagatgaag
     9181 cggcatttgc tacaggatat ccgtcgctca gcctacgttt atcgcgtcga ttgcggcggc
     9241 tgcaacgcct gtgaaatcga aatttttgct gccatcaccc ccgtattcga cgcagaacgt
     9301 tttggcatta aggttgtttc atcaccgcgt cacgccgata ttttgttatt tactggcgca
     9361 gtcacccggg cgatgcgtat gcctgcactt cgggcgtatg agtctgcccc cgatcataaa
     9421 atctgtgttt cctacggcgc gtgcggtgtc ggcggcggta ttttccacga tctctacagc
     9481 gtctggggcg gcagcgacac cattgtcccc attgatgttt ggatccccgg ctgcccgcca
     9541 acaccggccg ccaccattca cggtttcgcc gtggcgctcg gtttgctgca acagaagatt
     9601 cacgctgtgg attatcgcga tcccaccggg gtgactatgc aaccgttgtg gccacagatc
     9661 ccgccatcac agcgtatcgc cattgagcga gaagcgcggc ggctggcggg ctatcgtcag
     9721 gggcgagaaa tttgcgatcg gctcctgcgc catttaagcg acgatcctac aggaaatcgg
     9781 gttaacacct ggttgcgcga tgccgacgat ccacgtctca atagtatcgt tcagcaactc
     9841 tttcgcgtac tccgggggtt acatgactga agagtgcggg gaaattgttt tctggacgct
     9901 gcgaaaaaag tttgtcgcca gtagcgacga gatgccggaa caccgctctc aggtaatgta
     9961 ttactcgctg gctatcggcc atcacgttgg cgtgattgat tgtctgaatg tcgccttccg
    10021 ctgcccactg acggaaaacg aagattggct tgcacaggtc gaagaggagc aagcccgacg
    10081 taagatgctg ggggtgatga cctttggtga gattgttatt gacgccagcc acaccgccct
    10141 gttgacccgg gcattcgcgc tactggcaga tgacgcgacg tctgtgtggc aggcgcgtag
    10201 cattcaattc attcatctgt tggatgaaat tgtgcaggaa ccggccatct atctgatggc
    10261 cagaaaaatt gcgtgagaag gatttctcat taataaggac tgttgatggc tatgtcagac
    10321 gaggcgatgt ttgccccgcc acaaggaata acaattgaag cggtaaacgg aatgctcgcg
    10381 gagcggttag cacagaaaca cggtaaggcg tctttattac gcgccttcat cccgatgccg
    10441 ccgccgttca gcccggtaca acttattgaa ctgcatgtta tcaaaagcaa cttctattac
    10501 cgctaccatg atgatggcag cgatgtgacg gcaacaacag agtatcaggg cgagatggtc
    10561 gattattcgc gtcacgccgt ccttctcggc agtagtggaa tggcggagct acgctttatt
    10621 cgtacccacg gcagccgttt tactccctag gattgcacac tgtttaactg gctggcacgc
    10681 attatcactc cggttctgca atcatggctc aatgatgaag aacaacaggt ggcgctgcgt
    10741 ttgctggaga aagatcgcga tcatcatcgg gtactggttg atattactaa tgcagtgctg
    10801 tcacatcttg atctcgacga tctgatcgct gacgtcgctc gtgagatcca tcattttttc
    10861 ggtctggctt cagtcagtat ggtactgggc gatcatcgaa agaacgagaa gtttagcctg
    10921 tggtgcagcg atctttctgc ctcacattgt gcgtgtctgc cacgcaatat gcctggcgac
    10981 agtgtattgc tgacacaaac gctacaaacc cgacaaccga ccttgacgca ccgtgcagac
    11041 gatctgtttc tctggcaacg cgacccgtta ttactcttac ttgcatctaa cggctgcgaa
    11101 tctgcgctcc ttataccgct tacctttggc aaccatacac cgggtgcatt gttgctggcg
    11161 catacctctt ccactctctt tagtgaggaa aactgccagc tactacaaca catagccgat
    11221 cgcatcgcta ttgccgttgg caatgccgat gcctggcgta gcatgaccga tttgcaggaa
    11281 agtttgcagc aagaaaacta ccagcttagc gagcagctcc tttcgaatct gggcatcggt
    11341 gacattatct atcaaagcta ggcaatggaa gacctgctcc agcaggtaga tattgtagcg
    11401 aagagcgaca gtacggtgtt gatttgtggt gaaaccggaa ccggcaaaga ggtgatcgcc
    11461 agagcgctcc atcaacttag cacgcgacgc gacaaaccgc tggtcaaaat caactgcgct
    11521 gccatccccg ccagtcttct ggaaagtgag ttattcggtc atgacaaagg ggcgtttact
    11581 ggtgcgatta atacacatcg tggtcgtttt gaaattgccg atggcggcac gttgtttctc
    11641 gatgaaattg gcgatctgcc gttagaactt cagcctaaac tgctgcgcgt attgcaggaa
    11701 cgggagattg agcgtctcgg cgggagtaga acgatcccgg tgaatgtcag agtcattgcc
    11761 gccaccaacc gtgatttgtg gcaaatggtt gaagatcgcc agtttcgcag caatctcttt
    11821 tatcgcctga atgtcttccc attggaattg ccgccgctac gcgaccgtcc ggaagatatc
    11881 cctcttttag caaaacattt cacgcaaaaa atggcgcgcc atatgaatcg cgcaattgac
    11941 gccatcccga ccgaggcgct acgccagttg atgtcgtggg actggccggg caacgtgcac
    12001 gagctggaaa acgtgattga gcgagcggta ctgctgactc gcggtaacag tctgaattta
    12061 catcttaatg tccgacaaag ccgtttactg ccgacgctaa atgaagattc agcgcttcgc
    12121 agttcaatgg cgcagttgct gcacccgacg acgccagaga atgacgaaga agaacgtcag
    12181 cgcattgttc aggtattgcg agaaaccaat ggcattgttg ccgggccccg tggcgcggcg
    12241 acacgattag ggatgaagcg caccacgctg ctgtcacgaa tgcagcgtct ggggatctcg
    12301 gttcgcgagg tgttgtaatc tgcttttgca ggagtatgca tgagaaacaa actctctttc
    12361 gacttgcagt tgagcgccag aaaagcggca atcgctgaac ggattgccgt ccataaaatt
    12421 gcccgcagta aagtgtcggt ctttttaatg gcgatgtccg ctggcgtgtt tatggcgatc
    12481 ggatttactt tttacctttc cgttatcgcc gatgccccgt cttcacaggc attaacccat
    12541 ctggtgggcg gcctttgctt tacactcggc tttattttgc tggcggtttg cggcacagcc
    12601 tgttcacctc gtcggtaatg acggtgatag caaaaagtcg gggcgttatt agttggcgaa
    12661 cttggctgat taacgcactt ctggtggcct gtggtaatct ggcaggtatt gcctgtttca
    12721 gtttgttaat ctggttttcc gggctggtga tgagtgaaaa cgcgatgtgg ggagtcgcgg
    12781 ttttacactg cgccgagggc aaaatgcatc atacatttac tgaatctgtc agcctcggca
    12841 ttatgtgcaa tctgatggtt tgcctggcgc tgtggatgag ttattgcggg cgttcgttat
    12901 gcgacaaaat cgtcgccatg attttgccca tcaccctgtt tgtcgccagt ggctttgagc
    12961 actgtatcgc caatttgttt gtgattccgt tcgccattgc cattcgccat ttcgcccccc
    13021 ccttctggca actggcgcac agtagcgcag acaattttcc ggcactgacg gtcagccatt
    13081 ttattaccgc caatctgctc ccggtgatgc tgggtaatat tatcggcggt gcagtgctgg
    13141 tgagtatgtg ttatcgggct atttatttac gtcaggaatc ctgaaaaatc agcccggcga
    13201 aacagttcgt cgggctgaag attattcttg cgcgatttgc ccatcgggcc aggcgtggat
    13261 cacggctttg atcagcgtcg ccaatggaat ggcgaagaat acgccccaga atccccacaa
    13321 accaccgaag atcaccaccg ataaaataat caccagcgga tgcaggttaa ccgcttcgga
    13381 gaacaacacc ggtactagca ggttgccgtc cagcgcctga ataatcagat acaccgcgaa
    13441 gcagctccag aattccgtgc ctgcgccaaa ctggaatagc gccacgccaa ccaccggaat
    13501 ggtcaccaca aatgcgccaa tgtacggaat aagaaccgag aagccgacca gcaccgccag
    13561 cagcagcgaa tagttcagcc caaagagcaa gaaccccagc caggtggcga tccccaccac
    13621 gatcatctcc agcactttgc cgcggatata gttggtgatt tgttgattca tctccttcca
    13681 cacctgtcct gccagtccac ggttgcgcgg cagcacccga cgaacggcgt tcagcatctg
    13741 ctctttgtct ttcagcagga agaagaccat caatggcacc agcaccagat agacggcaat
    13801 ggtcagcaaa ccgaccagcg aggcgaggga aattttcacc accgaatcgc ccatggtcag
    13861 catccgactg cgcatatttt cggccattgc atcaataatg cccgcgtcca ttaacgccgg
    13921 atagcggcgc ggcaacgtgg cggcaaagtc agaaagctta ttgagcatcc ccggcatatc
    13981 gcggattaag tagatgccct gttgccaggc gataggcagt accacgaacg ccatcagtag
    14041 caatataccg acaaaaacca ccaatacaat cgacgtcgcc cagcggcggg agcagccaat
    14101 agattgcagg cgctcggttg gccattccag caaataggcc agcacaatag ccaccagcaa
    14161 cggagcaagc aggccactaa agaaaaagat aatgccaaat ccggcaacta aaataaccag
    14221 caaggcaatc gcttccgggt cgctaaaacg gcggcgatac cattgcatca acatttcgag
    14281 cataaaaacc ttccctgaac ctcaagagcg ggattgcgat ccgcaattgt atcgaaatgt
    14341 cacaaaaaag acttcgcttt ttatgacgga ttcaggaaac tgaaaagtca tttgagtggg
    14401 ctaatcttcg ccgttacact caaaggcggc gcggtgggaa cgatatttca cagtatcggt
    14461 caaatgacta tcttcagaaa tacaggatag aggttatgtt caggcagttg aaaaaaaacc
    14521 tggttgcaac cctcattgct gctatgacca ttggtcaggt agccccggcg tttgccgaca
    14581 acgcagacac cttgccggat atgggaacct ccgcaggaag cacgctttcc attggtcagg
    14641 aaatgcagat gggcgactat tatgtccgcc agctacgcgg cagcgcgccg ttaattaatg
    14701 acccgctgtt aacgcaatat attaattcgc tggggatgcg tctggtttcg catgccaatt
    14761 cggttaagac accgtttcat ttttttctaa tcaacaacga cgaaattaac gcctttgctt
    14821 tctttggcgg caacgtggtg ctgcactctg ccctgttccg ttattccgat aacgaaagtc
    14881 aactggcttc agttatggcg cacgaaatct cccacctcac ccaacgtcac ctggcgcgag
    14941 cgatggaaga tcagcagcgc agcacgccgc tgacctgggt cggcgcgtta ggttctattt
    15001 tactggcgat ggccagtccg caggcgggga tggcggcgct gaccggtaca ctggcgggaa
    15061 cgcgtcaggg gatgatcagt ttcacccagc aaaatgaaca ggaagcggac cgcattggta
    15121 ttcaggtgct gcaacgctcg ggattcgatc cgcaggcgat gccaaccttc ctcgaaaaat
    15181 tactcgatca ggcgcgttac tcctcgcgcc cgccggaaat tttattgact cacccgttgc
    15241 cggaaagtcg tctggcagat gcccgcaacc gtgctaatca gatgcgaccg atggtggtgc
    15301 agtcgtcgga agatttctat ctggcgaaag cgcgcacact ggggatgtat aattccggac
    15361 gtaaccagct caccagtgat ttgctggatg aatgggcgaa aggaaacgtt cgtcagcaac
    15421 gagcggcgca atatggtcgt gctttacagg cgatggaagc caataaatac gacgaggcgc
    15481 gtaaaacgct gcaaccgtta ctggcggcag aacctggcaa cgcatggtat ctcgatctgg
    15541 ctaccgatat cgatcttggg caaaacaaag ccaatgaggc aatcaatcgc ctgaaaaatg
    15601 cccgtgattt gcgcaccaat ccggtgttgc agctcaacct ggcaaacgct tatctgcaag
    15661 gcggtcaacc acaagaagcg gccaatattc tcaatcgcta cacctttaat aataaagatg
    15721 acagcaacgg ctgggattta ctggcacagg cggaagccgc gctaaataac cgcgatcagg
    15781 agctggctgc gcgagcagaa ggttatgcgc tcgccggacg actcgatcag gccatttcgc
    15841 tgttgagtag cgccagttcg caggtgaaat taggcagcct gcaacaagcg cgttacgatg
    15901 cgcgcatcga ccagttgcgc cagctgcagg aacgctttaa gccttatacc aagatgtaat
    15961 tcgcattaaa ggaagaacaa tgaccaaaca ggtaaaaatc taccataacc cacgctgttc
    16021 aaagagccgg gaaacgctga acctgctgaa agaaaacggc gtggagccag aagtggtact
    16081 ctaccttgag acacccgccg atgcggcaac gctgcgcgat ttgctgaaaa tgctggggat
    16141 gaatagcgcc cgtgaattga tgcgccagaa agaggatctt tataaagaac tgaacctggc
    16201 tgacagttcg cttagcgaag aggcgctgat tcaggcaatg gtggataatc cgaagctgat
    16261 ggagcgcccg attgtggtcg cgaatggcaa agcgcggatt ggtcgtccac cggagcaggt
    16321 actggaaatc gtgggttaag atgtgctcca tcgctagttg aagcacattg ccggatgcga
    16381 cgcttgccat gtcttatccg gcctacgaat actatggatt aacttcgcgt agttcggata
    16441 aggcgttcgc gccgcatccg acaataaaca ccttatctac aacttcagaa tttctttcac
    16501 aaacggaatg gtcagcttac gttgcgcggt aatcgacgca cgatccaact gatccaacgt
    16561 cataaatagc gtgcgcattt ctctgtcgag ccgcttcagc aagaaacgcc ccacatcttc
    16621 cggcagttca aaaccacgca aacgcgcgcg taactgtagc gcctgcaact tatcttcatc
    16681 agaaagtggc tgcaatttgt agatctgccc ccagtcgagt cgcgacgcga gatccggtaa
    16741 tcccagattc aactgccgcg gtggacgatc gccggtgatc aacaaccgtg ttttgcccga
    16801 ttccagaatt cgattgtaga gatcgaaaat cgccatctcc cacaactcat cgcctgcaat
    16861 acactcaatg ttgtcgatac aaaccagcga caaatgctcc ataccgtcga gcacttccgg
    16921 aacaaaccaa gtgcgtttat ccagcgggac atagcccacc gcatcgccac gctgcgacaa
    16981 ttccgcgcaa gccgcgtgca gcagatggct gcgccccgcg ccttcgcgtg cccagagata
    17041 gatgtaaccg ctatgttcct gacgcagcac gttttgcagc gcggccagta aagaggagtt
    17101 atcccccggc cagaaacttg caaaggtttc gtcgtcagga agataaagtg gcaaagagag
    17161 ctgtgccggt gtgttcagag atacctcaac caggatttca caaaatcgcg agaagtttac
    17221 cacagaatcc catgatgttt gaaccgggca gcaacactgc ccggtcggta cattatttgt
    17281 ctgttatatc cgcctcttct gcgtccagca ccacttcttc agggcgcagc acactgatca
    17341 gtttgaaaat caaacttaag ccgataccga cgatggtcgc cagcgccata ccttttaact
    17401 ccgccgcgcc gatgtttacc ttcgcgccac tgacgccgat gatcaaaatc acggaagtca
    17461 ggatcaggtt ctgtgctttg ttgtaatcca ctttcgattc gatcaaaaca cgaataccgg
    17521 aagcaccgat gacaccataa agcagcagcg aaacgccgcc catcaccggc aatgggatca
    17581 tctggatagc ggcagccagt ttaccgacgc aggaaagcag gatagcgaaa atcgccgccc
    17641 cgccgataac ccaggtactg taaacacggg tgatcgccat cacgccaatg ttttctccgt
    17701 aagtggtatt tggcgtggag ccaaagaagc cggagatcac ggtcgacagg ccgttagcaa
    17761 acatcgaacg gtgcagacct ggatcgcgca gcagatcttt tttgacgata ttagccgtta
    17821 ctaccaggtg ccctacgtgt tcggcaataa ccactaacgc cgctggcaga atagtcagaa
    17881 tggcaaacca ctcgaagcgc ggcgtataga gggttggcag cgcaaaccag tgagcattaa
    17941 taatcggcgt ggtatcgaca attcccattg cgaaagagag cgcgtagccc accagcacgc
    18001 caattaaaat cgggataatt gccaggaaac cacgaaacag cacggaacct aaaaccgtga
    18061 ccgccagggt ggtaatagag atgatgatgg ttttggagtc tggcgtttgc ccttcagccg
    18121 ggagtaaacc cgccataccg gcagctacgc ccgccagctc cagaccgatg acggcaacga
    18181 ttgcgcccat tgccgcaggt ggaaacagca cgtccagcca gccggtcccc gctttcttca
    18241 cgataaaaga aaccaggcag aacagcacgc cgcacataat aaagccgccc agcgcgactt
    18301 cataccctaa cggcaacagt aacaataccg gtgaaataaa ggcaaagctg gaaccaagat
    18361 aagccggaat tttcccttta cagatgaaga gatacagcag cgttccaata ccgttaaata
    18421 acagtacagt cgccgggtta atatgaaata agacgggcac caggacggtt gcaccaaaca
    18481 tggcgaacaa atgttgcaaa ctaagcggga ttgtctgtaa aagtggcggt ctttcactca
    18541 ccccgatagc acggcgcgtc atagtattat cctctgtatt atgtgttata ggcgctttac
    18601 tcaaaaaaaa gccgactctt aaagtcggct ttaattattt ttattcttta tttcgtacca
    18661 aagattttgt caccggcatc gccgaggccc ggaataatgt atccgtgctc gttcagtccc
    18721 tgatcaatcg atgcggtata cagttcgacg tccgggtgcg ctttttccag cgcagcgata
    18781 ccttctggcg cagcgaccag caccagaact ttgatgctgc tgcagcccgc ttttttcagc
    18841 aggtcgatgg tcgcgataac ggaaccaccg gttgccagca ttgggtcaac gatcagcgcc
    18901 atacgctcat cgatgttaga aaccagtttc tggaagtacg gtaccggctc cagcgtttct
    18961 tcattacggt acataccgac aacgctgatg cgcgcgctcg gaacattttc cagcacaccg
    19021 tccatcatac caagacccgc acgcagaatt ggcacaacgg taattttctt acctttgatc
    19081 tggtcgattt ctaccgggcc gttccagcct tcgatagtta ctttttccgt ttcgaggtcg
    19141 gcggtcgctt cgtaagtcag caggctaccc acttcggaag cgagttcgcg aaagcgcttg
    19201 gtgctgatat cttgctcacg catcagtccc agcttgtgtt tgacgagtgg gtgtttgact
    19261 tccacgatct tcatactctt tctcctttga ggggcagcca caaaaaaatc gacggattat
    19321 acctcctttc ttcaaggcgg caatattctt ttcgttgact ttagtcaaaa tgataacgat
    19381 ttgagataaa gttattttat attcaaatgg ttatgaaaga agatcattcc atccgaaaac
    19441 taacctttgc cctggcacaa gtcttctttc gccgcgcgtc tggggaaaag acgtgcaaaa
    19501 aggttgtgca aagcagcctc gcaaacgttt gctttccctg ttagaattgc gccgaatttt
    19561 atttttctac cgcaagtaac gcgtggggac ccaagcagtg accgataaaa cctctcttag
    19621 ctacaaagat gccggtgttg atattgacgc gggtaatgct ctggttggaa gaatcaaagg
    19681 cgtagtgaag aaaacgcgtc gtccggaagt gatgggcggt ctgggcggct tcggtgcgct
    19741 gtgtgcattg ccgcaaaaat atcgtgaacc cgtgctggtt tccggcactg acggcgtagg
    19801 taccaagctg cgtctggcaa tggacttaaa acgtcacgac accattggta ttgatctggt
    19861 cgccatgtgc gttaatgacc tggtggtgca aggtgcagaa ccgctgtttt tcctcgacta
    19921 ttacgcaacc ggaaaactgg atgttgatac cgcttcagcg gtgatcagcg gcatcgcgga
    19981 aggttgtctg caatcgggct gttcactggt gggtggcgaa acggcagaaa tgccggggat
    20041 gtatcacggt gaagattacg atgtcgcggg tttctgcgtg ggcgtggtag aaaaatcaga
    20101 aatcatcgac ggctctaaag tcagcgacgg cgatgttctg attgcactcg gttccagcgg
    20161 tccgcactcg aacggctatt cgctggtgcg caaaattctt gaagtcagcg gttgtgatcc
    20221 gcaaaccacc gaacttgatg gtaagccatt agccgatcat ctgctggcac cgacccgcat
    20281 ttacgtgaag tcagtgctgg agttgattga aaaggtcgat gtgcatgcca ttgcgcacct
    20341 gaccggcggc ggcttctggg aaaacattcc acgcgtattg ccagataata ctcaggcagt
    20401 gattgatgaa tcttcctggc agtggccgga agtgttcaac tggctgcaaa cggcaggtaa
    20461 cgttgagcgc catgaaatgt atcgcacctt caactgcggc gtcgggatga ttattgccct
    20521 gcctgctccg gaagtggaca aagccctcgc cctgctcaat gccaacggtg aaaacgcgtg
    20581 gaaaatcggt atcatcaaag cctctgattc cgaacaacgc gtggttatcg aataatgaat
    20641 attgtggtgc ttatttccgg taacggaagt aatttacagg caattattga cgcctgtaaa
    20701 accaacaaaa ttaaaggcac cgtacgggca gttttcagca ataaggccga cgcgttcggc
    20761 cttgaacgcg cccgccaggc gggtattgca acgcatacgc tcatcgccag cgcgtttgac
    20821 agtcgtgaag cctatgaccg ggagttgatt catgaaatcg acatgtacgc acccgatgtg
    20881 gtcgtgctgg ctggttttat gcgcattctc agcccggcgt ttgtctccca ctatgccggg
    20941 cgtttgctga acattcaccc ttctctgctg ccgaaatatc ccggattaca cacccatcgt
    21001 caggcgctgg aaaatggcga tgaagagcac ggtacatcgg tgcatttcgt caccgatgaa
    21061 ctggacggtg gcccggttat tttacaggcg aaagtcccgg tatttgctgg tgatacggaa
    21121 gatgacgtca ccgcccgcgt gcaaacccag gaacacgcta tttatccact ggtgattagc
    21181 tggtttgccg atggtcgtct gaaaatacac gaaaacgccg cgtggctgga tggtcaacgt
    21241 ctgccgccgc agggctacgc tgccgacgag taatgccccc gtagttaaag cgccagctct
    21301 gccgctggcg tttttcaatt cacctgtaaa tcgcaagctc cagcagtttt tttccccctt
    21361 ttctggcata gttggacatc tgccaatatt gctcgccata atatccaggc agtgtcccgt
    21421 gaataaaacg gagtaaaagt ggtaatgggt caggaaaagc tatacatcga aaaagagctc
    21481 agttggttat cgttcaatga acgcgtgctt caggaagcgg cggacaaatc taacccgctg
    21541 attgaaagga tgcgtttcct ggggatctat tccaataacc ttgatgagtt ctataaagtc
    21601 cgcttcgctg aactgaagcg acgcatcatt attagcgaag aacaaggctc caactctcat
    21661 tcccgccatt tactgggcaa aattcagtcc cgggtgctga aagccgatca ggaattcgac
    21721 ggcctctaca acgagctatt gctggagatg gcgcgcaacc agatcttcct gattaatgaa
    21781 cgccagctct ccgtcaatca acaaaactgg cttcgtcatt attttaagca gtatctgcgt
    21841 cagcacatta cgccgatttt aatcaatcct gacactgact tagtgcagtt cctgaaggat
    21901 gattacacct atctggcggt ggaaattatc cgtggagaca ccatccgtta cgcgctgctg
    21961 gagatcccat cagataaagt gccgcgcttt gtgaatttac cgccagaagc gccgcgtcga
    22021 cgcaagccga tgattcttct ggataacatt ctgcgttact gccttgatga tattttcaaa
    22081 ggcttctttg attatgacgc gctgaatgcc tattcaatga agatgacccg cgatgccgaa
    22141 tacgatttag tgcatgagat ggaagccagc ctgatggagt tgatgtcttc cagtctcaag
    22201 cagcgtttaa ctgctgagcc ggtgcgtttt gtttatcagc gcgatatgcc caatgcgctg
    22261 gttgaagtgt tacgcgaaaa actgactatt tcccgctacg actccatcgt ccccggcggt
    22321 cgttatcata attttaaaga ctttattaat ttccccaatg tcggcaaagc caatctggtg
    22381 aacaaaccac tgccgcgttt acgccacatt tggtttgata aagcccagtt ccgcaatggt
    22441 tttgatgcca ttcgcgaacg cgatgtgttg ctctattatc cttatcacac ctttgagcat
    22501 gtgctggaac tgctgcgtca ggcttcgttc gacccgagcg tactggcgat taaaattaac
    22561 atttaccgcg tggcgaaaga ttcacgcatc atcgactcga tgatccacgc cgcacataac
    22621 ggtaagaaag tcactgtggt ggttgagtta caggcgcgtt tcgacgaaga agccaacatt
    22681 cactgggcga agcgcctgac cgaagcaggc gtgcacgtta tcttctctgc gccggggctg
    22741 aaaattcacg ccaaactgtt cctgatttca cgtaaagaaa acggtgaagt ggtccgttac
    22801 gcacacatcg ggaccgggaa ctttaacgaa aaaaccgcgc gtctttatac tgactattcg
    22861 ttgctgaccg cagatgcgcg tatcaccaac gaagtacggc gggtatttaa ctttattgaa
    22921 aacccatacc gcccagtgac atttgattat ttaatggtgt caccgcaaaa ctctcgccgt
    22981 ctgttatatg aaatggtaga ccgcgaaatc gccaacgcgc agcaagggct gcccagtggt
    23041 atcaccctga agctaaataa ccttgtcgat aaaggcctgg ttgatcgtct gtatgcggcc
    23101 tccagctccg gcgtaccggt taatctgctg gttcgcggaa tgtgttcgct gatccccaat
    23161 ccggaaggca ttagcgacaa cattcgtgcc atcagtattg ttgaccgtta ccttgaacat
    23221 gaccgggttt atatttttga aaatggcggc gataaaaagg tctacctttc ttccgccgac
    23281 tggatgacgc gcaatattga ttatcgtatt gaagtggcga caccgctgct cgatccgcac
    23341 ctgaagcagc gggtgctgga catcatcgac atattgttca gcgatacggt caaagcacgt
    23401 tatatcgata aagaactcag taatcgctac gttccccgcg gcaatcgccg caaagtacgg
    23461 gcgcagttgg cgatttacga ctacatcaaa tcactcgaac aacctgaata accctatgcc
    23521 aatacacgat aaatcccctc gtccgcagga gtttgctgcg gtcgatcttg gttcaaacag
    23581 ttttcacatg gtcatagccc gtgtggtaga tggtgccatg cagattattg gccgcctgaa
    23641 acagcgagtg catctggcgg acggcctggg gccagataat atgttgagtg aagaggcaat
    23701 gacgcgcggt ttgaactgtc tgtcgctgtt tgccgaacgg ctacaagggt tttctcctgc
    23761 cagcgtctgt atagtcggca cccatacgct gcgtcaggcg ctgaacgcca ctgactttct
    23821 gaaacgcgtg gaaaaggtca ttccctaccc gattgaaatt atttccggta atgaagaagc
    23881 ccgtctgatt tttatgggcg tggaacatac ccaaccggag aaaggtcgca aactggttat
    23941 tgatattggc ggcggatcta cggaactggt gattggtgaa aatttcgaac cgattctcgt
    24001 tgaaagccgc cggatgggtt gtgtcagctt tgcccagctt tacttccctg gcggggtcat
    24061 caataaagag aattttcagc gcgctcgtat ggcggcagca caaaaactgg aaactttaac
    24121 ctggcaattc cgtattcagg gctggaacgt ggcaatgggc gcttccggta ccataaaagc
    24181 cgcccatgaa gtgttaatgg aaatgggcga gaaagacggg ataattaccc cggaacgtct
    24241 ggaaaaactg gtaaaagaag ttttacgtca ccgtaatttc gcatcgctga gtttaccggg
    24301 tctttccgaa gagcggaaaa cagtcttcgt tcctggactg gcgattttat gcggtgtgtt
    24361 tgatgcttta gccatccgtg aactgcgcct ttctgacggg gcgttacgcg aaggcgtact
    24421 gtacgaaatg gaaggacgtt tccgtcatca ggatgtgcgt agtcgcaccg ccagcagcct
    24481 cgccaaccag tatcacatcg acagcgaaca ggcccgacgg gtgctggata ccactatgca
    24541 aatgtacgaa cagtggcggg aacagcaacc gaagctggcg catccgcaac tggaggcgct
    24601 actgcgatgg gccgccatgc tgcatgaggt cgggttgaat atcaaccaca gcggtttgca
    24661 tcgccactcc gcttatattc tgcaaaacag tgacttgccg ggttttaatc aggaacagca
    24721 gctgatgatg gcgacactgg tgcgctatca ccgcaaagcg attaaactcg acgatctgcc
    24781 gcgctttacc ttgtttaaga agaaacagtt cctgccactg attcagctat tgcgccttgg
    24841 cgtattactc aacaatcaac gtcaggcaac caccacaccg ccaacattga cactgattac
    24901 tgatgacagt cactggacac tgcgtttccc gcatgactgg tttagtcaga atgcgttggt
    24961 actgcttgat ctggaaaagg agcaagaata ctgggaaggc gtggctggct ggcggttgaa
    25021 aattgaagaa gaaagtacac ctgaaatcgc cgcttaatgc tttgcgggcc gacgagaaat
    25081 gtcggcccgc attattcagg cactttcgcg aatgggtt
//
LOCUS       DAZPEB010000038        25005 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZPEB010000038 DAZPEB010000000
VERSION     DAZPEB010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25005
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.039"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..808
                     /locus_tag="ACHURJ_001414"
     CDS             <1..808
                     /locus_tag="ACHURJ_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFR1280427.1"
                     /translation="PRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTG
                     GRLLMFGVGVIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVIN
                     LTFTVLAIMTVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAF
                     AMSWGPVCWVLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHN
                     GFSYWIYGCMGVLAALFMWKFAPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(852..1250)
                     /gene="psiE"
                     /locus_tag="ACHURJ_001415"
     CDS             complement(852..1250)
                     /gene="psiE"
                     /locus_tag="ACHURJ_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFR1280428.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLIIVDHK
                     SPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            1520..1798
                     /locus_tag="ACHURJ_001416"
     CDS             1520..1798
                     /locus_tag="ACHURJ_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1280429.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(1845..3941)
                     /locus_tag="ACHURJ_001417"
     CDS             complement(1845..3941)
                     /locus_tag="ACHURJ_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFR1280430.1"
                     /translation="MKKRHLLSLLALGISMACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNVGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     MADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVLKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(3941..4678)
                     /locus_tag="ACHURJ_001418"
                     /pseudo
     CDS             complement(3941..4678)
                     /locus_tag="ACHURJ_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
     gene            complement(4675..5313)
                     /locus_tag="ACHURJ_001419"
     CDS             complement(4675..5313)
                     /locus_tag="ACHURJ_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFR1280431.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKILLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(5427..5669)
                     /gene="yjbE"
                     /locus_tag="ACHURJ_001420"
     CDS             complement(5427..5669)
                     /gene="yjbE"
                     /locus_tag="ACHURJ_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFR1280432.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(6131..7780)
                     /gene="pgi"
                     /locus_tag="ACHURJ_001421"
     CDS             complement(6131..7780)
                     /gene="pgi"
                     /locus_tag="ACHURJ_001421"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFR1280433.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     regulatory      8017..8213
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            8305..9654
                     /gene="lysC"
                     /locus_tag="ACHURJ_001422"
     CDS             8305..9654
                     /gene="lysC"
                     /locus_tag="ACHURJ_001422"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFR1280434.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNDGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            9906..10178
                     /locus_tag="ACHURJ_001423"
     CDS             9906..10178
                     /locus_tag="ACHURJ_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFR1280435.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(10311..11183)
                     /gene="rluF"
                     /locus_tag="ACHURJ_001424"
     CDS             complement(10311..11183)
                     /gene="rluF"
                     /locus_tag="ACHURJ_001424"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFR1280436.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            11531..12479
                     /locus_tag="ACHURJ_001425"
                     /pseudo
     CDS             11531..12479
                     /locus_tag="ACHURJ_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
     gene            12549..13355
                     /locus_tag="ACHURJ_001426"
     CDS             12549..13355
                     /locus_tag="ACHURJ_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR1280437.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALVWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            13365..13772
                     /locus_tag="ACHURJ_001427"
     CDS             13365..13772
                     /locus_tag="ACHURJ_001427"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFR1280438.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGDARVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            13772..14266
                     /locus_tag="ACHURJ_001428"
     CDS             13772..14266
                     /locus_tag="ACHURJ_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFR1280439.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIDTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            14332..15129
                     /locus_tag="ACHURJ_001429"
     CDS             14332..15129
                     /locus_tag="ACHURJ_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFR1280440.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSAFGVQALRVAIPALIVSL
                     FVSADMVSNMLSAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            15140..15982
                     /locus_tag="ACHURJ_001430"
     CDS             15140..15982
                     /locus_tag="ACHURJ_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000437910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFR1280441.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITTALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VTMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLSRF
                     FAPA"
     gene            16016..17245
                     /gene="sorE"
                     /locus_tag="ACHURJ_001431"
     CDS             16016..17245
                     /gene="sorE"
                     /locus_tag="ACHURJ_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HFR1280442.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRMLILGGTGPMGLLAIDYALHGPVNPSQLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLTHAEAISHD"
     gene            17628..18317
                     /gene="pepE"
                     /locus_tag="ACHURJ_001432"
     CDS             17628..18317
                     /gene="pepE"
                     /locus_tag="ACHURJ_001432"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFR1280443.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(18408..20039)
                     /locus_tag="ACHURJ_001433"
     CDS             complement(18408..20039)
                     /locus_tag="ACHURJ_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFR1280444.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTAALTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQSDEDEGRDEY"
     gene            complement(20259..23942)
                     /gene="metH"
                     /locus_tag="ACHURJ_001434"
                     /pseudo
     CDS             complement(20259..23942)
                     /gene="metH"
                     /locus_tag="ACHURJ_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
     gene            24142..24966
                     /gene="iclR"
                     /locus_tag="ACHURJ_001435"
     CDS             24142..24966
                     /gene="iclR"
                     /locus_tag="ACHURJ_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFR1280445.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMEVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         5880 a         6611 c         6534 g         5980 t
ORIGIN      
        1 tcctcgctgg ctgatgtcac gcggcaagca agaacaggcg gaaagtatcc tgcgcaaaat
       61 tatgggcaac acgcttgcaa ctcaggcagt acaggaaatt aaacactccc tggatcatgg
      121 ccgcaaaacc ggtggtcgtc tgctgatgtt tggcgtgggc gtgattgtca tcggcgttat
      181 gctctccatc ttccagcaat ttgtcggcat caatgtggtg ctgtactacg cgccggaagt
      241 gttcaaaacg ctgggggcca gcacggatat cgcgctgttg cagaccatta ttgtcggagt
      301 tatcaacctc accttcaccg ttctggcaat tatgacggtg gataaatttg gtcgtaagcc
      361 actgcaaatt atcggcgcac tcggaatggc aatcggtatg tttagcctcg gtaccgcgtt
      421 ttacactcag gcaccgggta ttgtggcgct actgtcgatg ctgttctatg ttgctgcctt
      481 tgccatgtcc tggggtccgg tatgctgggt actgctgtcg gaaatcttcc cgaatgctat
      541 tcgtggtaag gcgctggcaa tcgcggtggc ggcccagtgg ctggcgaact acttcgtctc
      601 ctggaccttc ccgatgatgg acaaaaactc ctggctggtg gcccatttcc acaacggttt
      661 ctcctactgg atttacgggt gtatgggcgt tctggcagca ctatttatgt ggaaatttgc
      721 cccggaaacc aaaggtaaaa cccttgagga gctggaagcc ctctgggaac cggaaacgaa
      781 gaaaacacaa caaactgcta cgctgtaatc ttcctgtcca gcacgccgcg ccagttcggc
      841 gtgctgtctt tttactcccg cttcagccgt ttcgaattac acagccacag agtgatcacc
      901 agtaacagga tcgcagccga gtagatcaac acatccagtg gcgatttatg atcgacgatg
      961 atcaagcgca caatggcggt gatcccaata tagacaaagt aacgtagcgg gaagtgaaaa
     1021 ccggactgaa agtacttcac aatcagcgcg ataaattcga aatagagaaa gtaaaccacc
     1081 agctcatatt tgctggtttg ttctggcgcg aacagcacat ctgccagatg caccgtttct
     1141 ttgccgagga agacaaccaa aatcaggccg aggcacagca ggccaagatt gagtacggtc
     1201 tgcaaaatgg tggagataaa ctccacgcgc ggacgagaga gtgacgtcat agcagtacct
     1261 ccttcacagt ggcgagcttc ctgtataacg caaaaatgtg acggagatct atattttttg
     1321 tgtatgtttt gttcaacctg gatttggagc gcgcctccac caaagccgga tatacctacc
     1381 cgtaggccgg ataagccgcg cctggcggcg catccggcaa gacaaaccgc acccaagccg
     1441 gtaattcaaa aatcacccat tcaggtgatt cccagccacc agcaatgcgc gaaaaatcga
     1501 caatgttcct ctggaacaaa tgaaacgtaa cctgataaag gtggtaaaaa tgaagcctta
     1561 ttttgctgct ttgatgttat cagtctctgt tcttcctgct tatgctggtc cgctcggtac
     1621 tgctgataaa gccgacctac cgcaaagcaa tgtctccagc ccaatgatgg cccagtcgct
     1681 caggcaacca gacctgcaac cgatatcgac ggacaggaag acagaatgtt ttcgccttta
     1741 cacaccagat cgcaaaccgg gggtgaactg cgttcctgat ggctcgacag gccattaaga
     1801 atgaaggtgc cagtgagtgc gaaaactggc acctttccca tgacttagcg gaaattgacg
     1861 ctacggtcgc tggtcatgtc atacaactgg aacttacgcc caagttgctg accaccgtca
     1921 cgcgtcagcg gcgtccagcc aatcgccgca cggctgcgtg tcgggccaga cgagaagaga
     1981 tccaacggta ccgagacata cacgcctttg gtgaagtccc cttcgccgta ctcctctttc
     2041 aaaacgttgg tgatcgtggc atagccaccc accaccacgc cgctgtcaaa gcgtttggcg
     2101 atctccagcg tgccgccttt atcccctgcc agatactgcc cgacgctggc tttcaccaac
     2161 acgtcctggg cgaaagatgg cgtccagtag gcggtcagat gtccggtttt cacgctgtag
     2221 tcggtgaatt tcatcatatc ttttgcacta cgccagtcgc gctgtttaac gtagttggca
     2281 tccagaccaa acgcccagtt actgtccagc ggacgataca acacttcggc ccccgcaccg
     2341 ccaaacatgg tttcgagata acctccatag acctgaccgt agaagccgtt gcccagatgc
     2401 tggaagtagt tggcttgcag gttattcaca tagacatcgt tctgcacata ctcgcgcaca
     2461 tgggtacgaa cgcgcggtaa gtgcgagtcc tgcggcggat tggtgtagtt aaacttgtcg
     2521 tagttgttgg caagatttgc aaacaggctg ccggtggtca gcagatggtc cgtcagccac
     2581 aaatctgcca ttcccatcac gcccagctga tacatgtaaa agttttccgg gccaccgacc
     2641 gactggttca gcaccggatc gatatggaaa tcaaagcgtg atttgtcgat ataccagccc
     2701 tgctcggtgg actgcggaac cactggctcg acgcgttttt gcgccagctt cgtttcgtgg
     2761 cccaacggct ctccggcgag atgatttttc aggctggcga catcggtttc cgtcgtcacc
     2821 tgcggcatgt taagacgatt ttccgtaatg cggatcgttt tgatcccatc cggcagatcg
     2881 ttcatcacga tccgattagc acggatgatc ccttcgcgcg aatcacgata tttcacctgc
     2941 tcgccggtga catacagcgt atcgcctttc gcctggatct gcggatcggc aagtccgaca
     3001 ttgtatttca acagcgttaa ctgattcgcc accaccgaat gctgcaaaat ggcatcctgc
     3061 ggctgcggtt gatattgcgg gcgggcgtta tcgttgtaag acgggcgcag atcgttaaag
     3121 ttggtgcgca gcgtgacgcc aaacataaag gtgttgccac gttcatagct aaggttaacg
     3181 tcggcccaat cggtaacgcg ataaatcgca ccgacgttaa acttactttt ttgctccagc
     3241 ttcccggcaa aatcctgctg ataattattg ccttcatact ccagtttcag gcgcagcggt
     3301 tgccagggcg tctggtattc cacgccgcca aacagtgagg caggaccgtg gaacatctgg
     3361 ctaccgtcga tagatcccgc ctgtttgtag ctgttatcgc gatagcaata tttatcactg
     3421 gctgaacaga gcggattttt cacattaccg ctggtgccca gataacccca gcccaggccg
     3481 agcgtaaaat cgaacggccc ccaggctttg ctggcaacaa gatattccgc atcaaacagc
     3541 cccgtaccgc cgatatccct cgcaccaacc gccacttgcg gcagccagta actctcttcc
     3601 cacaaacgca gtttgagatc gaaggcttta tctttatacg tttgatcgcc agagaacgct
     3661 tcgacgctgc tgtactgccg ggtgcgcacg tcggtgtagc gcagcgttgt ttccagccac
     3721 gggaagagtt gcactgaagc tgagtaataa cggtactgat cgttatcgcg atagttcagg
     3781 ctcaactccc cttcccgcgc catgcgtgcg gtgggcgttt gtaataatcc tacgccaccg
     3841 aaatccgact gcgacgggcc aatgggcgca ggatatgttt cgccgtagca agccatgcta
     3901 atgcccagcg ccagtaagct aagcagatgt ctttttttca ttattggggt atccgctgcg
     3961 tcagagtctg aagaatgtcg gcgttaaggg catcaggcgt ctcactccag acggagtccg
     4021 cgaggccaac ataaataatg ctgccgggca tcggctctac gtgacgcttg ttccagtaag
     4081 ccaccggcgc tttttgcgtg cgtccgtccg ggtaaaccac ccacgcgtag ctgcgatccg
     4141 caccgctaag caggttctgc ccggagagat agctcgccac gtcgcgacct ggagtgaatg
     4201 gctgcttgcc aggacggctg ataagcccga acaacgtgac cgtggacggc ggtggtccga
     4261 cccacagcgt gtagttgccc tgcaacggtg ggttaccgcg ttcggcaacg cgcacgatat
     4321 cgggatcaag attgattttt tgcctgcccg tcactttcaa cgcctgaatt tgctggcgta
     4381 aggcgttaat ggcggcggca tcgtcggcgc tggagtctgc ggcctgttca gccagccgtg
     4441 tcagcaacgc ctgctgctga cgcaatgctg ccgccgttgc cagctcttcg ctaatcaccg
     4501 caccgggcca ccagttgttt gccagccgcg gttagcctac cagatcgatt aaatgctctg
     4561 cgcctgttag tgttttggcc tcactgctgc cattgctgaa caccttgacg gttcctgccg
     4621 cgaagaccga tgacgctccc acactcaaaa gcaacgcgac aaaagtttgt ttaatcattg
     4681 ttttgccgcc ttgatgagag tggttttcac cgggaaataa tccgcgccga gatactgttc
     4741 cgactggcga atttgccctt cgctgtcgat ccagtagcgg ttatgccagc gcgcctggtc
     4801 ggtggagact tcttcgtcca gcacgcgaac cagggtttca tcgctgccga ctttgacggt
     4861 atcggtgcca tcccatttga agactgagcg tgcggtggcg tagcgtacct gctggtactc
     4921 ggtccagccc atcgtgcgcg tccatgttgc accatcaaca atttgtgcgg gtttaatcag
     4981 cgggtcggcg gcaaggttat tcacttcaat caggttgtcg ccgccaagca aaatcttcac
     5041 cagacgacca tgttgtgtga caagagtggc ttgatcctga gtgacccatt tttgttgtcc
     5101 gtcttcagcg aaggccagca ccacaaacaa ttgcggcccg ccattaagct gcatgtactg
     5161 gctggcgtag ggcatatttt gaatatcatc gtccgtcagc tgtacgcctg gcgtgccgaa
     5221 cagactgtcc cacagtgaat tgcccagctc tttagtcgtg gctgaacagg cctgtaatag
     5281 caggcagata agaatgagtg caggtcgctt cacgactctt ctccagaggg gcagaataac
     5341 cacaccggag tgtggttatg gtaaatccct tcatacttca agccacagat gcaatgactc
     5401 aaattatttt ggggatacat actttattac tgggtactgg tggtcgtggt tgtggtggtc
     5461 ccggtattag aaccatcacc gccaccggtt gccgcgagcg cgacacccac cgccgagctt
     5521 acggtgctga cgctggtcgc ggaggaacta cccgccgaaa ccgacgttgc tgccgaccct
     5581 gccgcttcgc ccacctgtac aggtgcagcc cacgcagaag tcgccgcaag cgcagatatg
     5641 gcaaaaatgc catacagaac ttttttcata acaatttccc ttcattgaat gaatggagat
     5701 ttacccaaaa acatttcggg cgggatcgag tatacacaac taaagcatgc gaattacggc
     5761 gcggggaatt agcgatggcg ttttaggata attggaatga atatcaaata taagcttcaa
     5821 cttatattta aataacaata aaaatcataa acttaaaata aaaacaaaag taattaatct
     5881 gcgtattctc cgaaataaac aacgattaaa aatctatttt aggattaacc tcacggtatg
     5941 atttccgtta aatcacagac aaggggattt atctgataaa acaatgccga cttattgtcg
     6001 gcattgtttt taaggttatt taactcctca tactatgcat taatgtcgat attggcatca
     6061 ggctccggtt gccggatgcg gcgtaaacgc cttatccggc ctacatatcg gcatcaggca
     6121 cgcatgatga ttaaccgcgc cacgctttat agcggttaat cagaccattg gtcgagctat
     6181 cgtggctgct gatttctttg tcatctttca gctctggcag aatacggttc gccagttgtt
     6241 tacccaactc cacgccccac tggtcgaagg taaagatgtt caggatcacg ccctgagtaa
     6301 agattttgtg ctcatacagc gcaatcaacg cccccaggct gaacggagtg atttcgcgca
     6361 gcaggatgga gttggtcggg cggttacctt cgaacacttt gaacggcacc acgtagtcaa
     6421 gcgttgccgg atctttaccc tgatcgcgat attcctgctc aaccacttcg cgggatttac
     6481 caaacgccag cgcttcggtc tgggcgaaga agttagacag cagtttctgg tggtgatcag
     6541 agagcgggtt atgggtgata gccggggcga tgaaatcgca cggtaccatt ttggttccct
     6601 ggtggatcag ctggtagaac gcgtgctgac cgttagtgcc tggttcaccc cagataatcg
     6661 ggccagtctg gtaatccaca acgttaccgt tacggtcaac atacttaccg ttggactcca
     6721 tattgccctg ctggaagtac gccgcgaaac ggtgcatata ctggtcatac ggcagaatcg
     6781 cttcagtttc cgcaccaaag aaattgttgt accagatgcc aatcagcgcc agcaggacag
     6841 gcaggttttt ctcggcaggc gtggtggaga aatgcttgtc catcgcgtgt gcgccagaaa
     6901 gcagttcaac gaagttatca aagccgatgg agagaacaat cgacaggcca atcgctgacc
     6961 acaaagagta acggccgccg acccagtccc agaactcgaa catgttggca gtatcaatac
     7021 caaactcgcc aacggctttg gcattggtgg aaagcgccgc aaagtgtttt gcaacgtgtt
     7081 tttcatcacc tgccgctttc aggaaccagt cacgcgcgct atgggcgttg gtcatagttt
     7141 cctgagtggt gaaggtttta gatgctacca agaacagcgt ggtttccggg tttacttttt
     7201 tcagcacttc agcgatgtga gtcccatcga cgttagaaac aaagtgcatg ttcaggtgat
     7261 ttttgtacgg acgcagtgct tcggtcacca tgtacgggcc gaggtcagaa ccaccgatcc
     7321 cgatgttcac tacgtcagtg attgctttgc cggtataacc cttccactca ccggagataa
     7381 tcgcttccga gaaggttttc atcttctcca gcaccgcgtt gacttccggc attacgtctt
     7441 tgccatcaac cagaatcggg gtattgctac ggttacgcag cgctacgtgc agcacggcgc
     7501 ggttttcagt gcggttgatc ttctcgccag agaacatcga cttaatcgcg cccgccagat
     7561 cgcactcttt cgccagatcc tgtaatttcg ccagcgtctc ttcagtgatg cggtttttgg
     7621 agtaatccac cagcatctga tcgtcgaatg ttgcggagaa cttagaaaaa cgatcgccgt
     7681 ctttagcaaa aagatcggcg atcgtaacgt ctttcatttc atcgaagtgt ttctgtagtg
     7741 cctgccaggc agcggtctgc gttggattga tgtttttcat tagcaatact cttctgattt
     7801 tgagaattgt gactttggaa gattgtagcg ccagtcacag aaaaatgtga tggttttagt
     7861 gccgttagcg taatgttgag tgtaaaccct tagcgcagtg aagcatttat tagctgaact
     7921 actgaccgcc aggagtggat gaaaaatccg catgaccccg tcgttgacaa ccgccccgct
     7981 caccctttat ttataaatgt actacctgcg ctagcgcagg ccagaagagg cgcgttgccc
     8041 aagtaacggt gttggaggag ccagtcctgt gataacacct gagggggtgc atcgccgagg
     8101 tgattgaacg gctggccacg ttcatcatcg gctacagggg ctgaatcccc tgggttgtca
     8161 ccagaagcgt tcgcagtcgg gcgtttcgca agtggtggag cacttctggg tgaaaatagt
     8221 agcgaagtat cgctctgcgc ccacccgtct tccgctcttc ccttgtgcca aggctgaaaa
     8281 tggatcccct gacacgaggt agttatgtct gaaattgttg tctccaaatt tggcggtacc
     8341 agcgtagctg attttgacgc catgaaccgc agcgctgata ttgtgctttc tgatgccaac
     8401 gtgcgtttag tagtcctctc ggcttctgct ggtatcacta atctgctggt cgctttagcg
     8461 gaagggctgg aacctggcga gcgattcgaa aaactcgacg ctattcgcaa catccagttt
     8521 gccattctgg aacgtctgcg ttacccgaac gttatccgtg aagagattga acgtctgctg
     8581 gagaacatta ctgttctggc agaagcggcg gcgctggcaa cgtctccggc gctgacagat
     8641 gagctggtca gccacggcga gctgatgtcg accctgctgt ttgtcgaaat cctgcgcgaa
     8701 cgcgatgttc aggcacagtg gtttgatgta cgtaaagtga tgcgtaccaa cgaccgattt
     8761 ggtcgtgcag agccagatgt agccgcgctg gcggaactgg ccgcgctgca gctgctccca
     8821 cgcctcaatg acggcttagt gatcacccag ggatttatcg gtagcgaaaa taaaggtcgt
     8881 acaacgacgc ttggccgtgg aggcagcgat tatacggcag ccttgctggc ggaggcttta
     8941 cacgcatctc gtgttgatat ctggaccgac gtcccgggca tctacaccac cgatccacgc
     9001 gtagtttccg cagcaaaacg cattgatgaa atcgcgtttg ccgaagcggc agagatggca
     9061 acttttggtg caaaagtact gcatccggca acgttgctac ccgcagtacg cagcgatatc
     9121 ccggtctttg tcggctccag caaagaccca cgcgcaggtg gtacgctggt gtgcaataaa
     9181 actgaaaatc ctccgctgtt ccgcgcgctg gcgcttcgtc gcaatcagac tctgctcact
     9241 ttgcacagcc tgaatatgct gcattctcgc ggcttcctcg cggaagtttt cggcatcctc
     9301 gcgcggcata atatttcggt agacttaatc accacgtcag aagtgagcgt ggcattaacc
     9361 cttgatacca ccggttcaac ctccactggc gatacgttgc tgacgcaatc tctgctgatg
     9421 gagctttccg cactgtgccg ggtggaagta gaagaaggtc tggcgctggt cgcgttgatt
     9481 ggcaatgacc tgtcaaaagc ctgcggcgtt ggcaaagagg tattcggcgt actggaaccg
     9541 ttcaacattc gcatgatttg ttacggcgca tccagccata acctgtgctt cctggtgccc
     9601 ggcgaagatg ccgagcaggt ggtgcaaaaa ctgcatagta atttgtttga gtaaatactg
     9661 tatggcctgg aagctatatt tcgggccgta ttgattttct tgtcactatg cttatcaata
     9721 aacgagcctg tactctgtta accaacgtct ttatcgagaa taattgcctt taattttttt
     9781 atctgcatct ctaattaatt atcgaaagag ataaatagtt aagagaaggc aaaatgaata
     9841 ttatcagttc tgctcgcaaa cgaattcccg tacactgaac tctctggctc actgagggca
     9901 ccatcatggc actcccccgt attacccaaa aagagatgac tgaacgcgag cagcgtgaac
     9961 ttaaaacact gctggatcgg gcgcggattg cgcatggtcg cgtactgacc aactccgaaa
    10021 ccaacagcat taagaaagag tacatcgata aattaatggt cgagcgtgag gctgaagcga
    10081 aaaaagcccg ccaattgaag aaaaaacagg cttataaacc ggatcctgaa gcgtcattta
    10141 gctggtcagc aaatacgtcg acgcgcggca ggcgctaatg aacaatcaac gcctgagatt
    10201 ttcactcgtc tgtgtggaca caatcttccc ctcaccctaa ccctctcccc gaaggggcga
    10261 ggggactgtc cgagctcttt ttggacgttt tcatcagtct gaggcgttaa tcagcgtccc
    10321 ttctttttac gccccggcga ggtaaaacgc ttaccgttgg acgccgggcg accgcctttt
    10381 tccgccgttt tttccatctt aacgactgga cgtttgatgc ccgctgtttt cggtttggcc
    10441 ttcgctttag gttttgcctc ggaagaggaa ttttcgatga gcttaaagag gtcgattaac
    10501 tcatcgtcgg ttaaatcgcg ccactccccc agcggaatgc cgcttaagct gacgttcata
    10561 atgcgcgtac gttccagctt tttcacttca tagccgaaat gctcgcacat gcgccggatc
    10621 tgacggttca gcccctgcac cagggtaatg cggaagacaa acggcgcttc ttttttgact
    10681 ttgcactttt tcgtcaccgt cccgaggatc ggcacccccg cccccatacc acgaataaat
    10741 tcatcggtaa tcggtttatc gaccgtcacc agatactctt tctcatgatc gttacccgca
    10801 cggaggatct tattcaccag atcgccgtga ttggtgagga aaatcagccc ctgggagtct
    10861 ttatccaggc ggccaatcgg gaacacgcgt ttgctgtggt taacgaaatc gacaatgtta
    10921 tcgcgctcgc catcttcggt ggtgctgaca ataccgacgg gcttgttcag ggcgataagt
    10981 accaaatctt cggcttcccg aggctcaatc aactgaccgt ttactttcac aatgtcgccg
    11041 ggtttcacct gatcgccaat ggtggctcgc ttgccattaa ggaacacatt gccttgctcg
    11101 atatagcgat ccgcttcgcg gcgtgagcaa attccgcttt cgctgatgta tttatttaaa
    11161 cggactgatg agtcgggcag cataaattct cctgtaaaac gcggaatata ccgtacctct
    11221 tagcggggaa aaacagggtt atgcggcaac gtcgaaatta aatgtgatct gccacgcctt
    11281 ttatcacaaa agtgtctccg gaagtacgat gcaaatttgc ctggcaaaat aaggataatc
    11341 aacatattac gaatcgctgc acacctgtgc tgatgactac tacgcagaga aacaaatgtg
    11401 caagagaaag cgctatatct ccccatgcaa agagtgaaaa atcgctttga aaggccctca
    11461 tgatggggtt atatcagatc aagcggtgag aaagcgtatt tgcacaaatg tgcagaacca
    11521 ggaggcggca atggaaaaca gtgacgatat ccgtttgatt gtgaagattg cccaactcta
    11581 ttacgaacag gatatgacgc aggcgcaaat cgcgcgcgaa ctgggtattt accgcaccac
    11641 catcagccgc ttgcttaaac gaggccgcga tcagggaatt gtcaccatcg ccatcaacta
    11701 tgactacaac gaaaatctct ggctggagca gcaactgaag caaaagtttg gcctgaaaga
    11761 cgttgtggtg gtgtcgggta aatgatgagg atgaagagac tcaactggcg atgatggggt
    11821 tacacggcgc gcaactgctg gatcgcttgc tggagcctgg cgatattgtc ggtttttcct
    11881 ggggtcgcgc ggtgagcgca ctggttgaaa acttgccgca ggcggggcaa tcgcggcagt
    11941 taatctgcgt accaattatt ggcggaccgt ccggtaaact cgaaagccgc tatcatgtaa
    12001 acacattaac ctacagcgcg gcagcgaagc tgaaagggga atcgcatctc gcggattttc
    12061 cggctctgct ggataaccca ttaattcgta atgggatcat gcagtctcag cactttaaaa
    12121 ccatctctgc ctactgggat aatctggatg tcgccctggt gggaattggc tcaccggcca
    12181 ttcgcgacgg cgctaactgg catgcgtttt atggtggtga agagagtgac gacctgaatg
    12241 cccgccaggt tgctggcgat atttgctcgc gcttttttga tattcacggc gcaatggttg
    12301 aaacgaatat gagcgaaaaa acactctcta tcgaaatgaa taaattaaag caggcacgat
    12361 attccattgg cattgccatg agtgaagaaa aatacagcgg aattgttggt gcactgcgtg
    12421 gaaaatatat taattgtctg gtaacgaata gcagcacagc tgaactatta ctgaaataaa
    12481 aatcttcccg atgtttagcg taataaaaat tacggcgggg ccctattatc taaaatcagg
    12541 agtctgttat gcaaacgtgg ttaaatttgc aggataaaat cattattgtc accggcggcg
    12601 catccggtat tggtctggcg attgtagagg aattattagc acaaggcgca aacgtacaga
    12661 tggtagatat tcacggtggc gatggtcaat atgaaggtca taaaggctat cagttttggc
    12721 cgaccgatat ttccagcgcc aaagaggtaa atcatacggt agcggaaatt atccagcgtt
    12781 ttggtcgcat cgacggtctg gtcaataacg ccggggtcaa tttcccgcgt ctgctggtcg
    12841 atgagaaagc gcctgccggg cagtatgaac tcaacgaagc tgcattcgaa aaaatggtca
    12901 atatcaacca gaaaggcgtt tttctgatgt cgcaggcggt ggcgcgacag atggtcaaac
    12961 aacatgatgg cgtgattgtg aatgtttcct cagaaagtgg actggaaggc tcagaaggcc
    13021 aaagctgtta cgccgctacc aaagccgcgc ttaatagctt cacgcgatcc tggtcgaaag
    13081 aattgggtaa acacggtatc cgtgtggtcg gtatcgcgcc ggggattctg gaaaaaacag
    13141 gactgcgcac gccggaatat gaagaagcgc tggtgtggac gcgcaatatc accgtcgagc
    13201 agctgcgtga aggctatacc aaaaacgcca ttcctattgg gcgcgccgga agattagcag
    13261 aagtcgctga ttttgtttgt tatctgctgt ctgaacgcgc cagctatatc accggagtaa
    13321 ccactaacat tgcgggcggc aaaacgcgcg gctaaggagg ctttatggtc aatgccattt
    13381 tttgcgccca cggcaaatta gcttgcgcca tgctggaatc ggttcagatg gtgtacggcg
    13441 acgccagggt cgaggcagtt gaatttgtgc ccggcgagaa cgccggagac atcgtagcaa
    13501 agctggaaaa gttagtaagc attcacaatc aagatgagtg gctgattgcc gtggatttac
    13561 agtgcggcag cccgtggaat gcggcagcga tgcttgcgat gcgcaatcct cgtctgcggg
    13621 taatcagcgg cctttctctg ccgctggcgc tcgaactggt tgataaccag gacagcatga
    13681 atgttgatga actgtgtgaa cacctgacgc aaatcgcaaa gcaaacctgc gttgtctgga
    13741 aacaactggc aacggcagag gaggatttct gatgaacatt acgctcgccc gcattgatga
    13801 ccgcctgatc cacggtcagg tcactaccgt ctggtcgaaa gtggctaacg cccagcgcat
    13861 tattatctgc aatgacgaag tttataacga tgaagttcgc cggacattat tgcgtcaggc
    13921 tgcgccaccg ggaatgaagg ttaacgtggt caatattgaa aaagccgtcg ctgtttatca
    13981 caacccgcaa tatcaggacg aaaccgtttt ttatcttttc actcgcccac aggatgcctt
    14041 agcgatggtt cgccaagggg taaaaataga caccctaaat attggcggca tggcctggcg
    14101 gcccggtaaa aaacagttaa ccaaagcggt ttcattagat gatgatgata ttaacgcatt
    14161 tcatgagtta aataatcttg gcgtcatact ggatttacgc gtcgtagcgt cagatccctc
    14221 aatcaatatt atcgacaaaa taaacgaaca gttaatcgca aattaaataa tagcgcttgt
    14281 gtacatatgc tttaacaggc agggatgacg acacttaaaa ggtgaaaaat catggaaata
    14341 agtaccctac aaatcattgc tatatttctt ttttcctgta ttgccggaat gggcagcgtg
    14401 ctggatgaat ttcagactca tcgcccgtta attgcctgta cggtgattgg tttaattctc
    14461 ggtgatttaa aaaccggaat aatactcggt ggtacgctgg aattgatagc tctcggctgg
    14521 atgaacgtcg gcgcggcgca atctccggat tctgcactcg ccagcataat ctccgccatt
    14581 ctggttatcg ttggtcagca aagcatcgcc accggaatcg ccatcgcgtt gcctgtggct
    14641 gcggcaggcc aggtgctgac tgtgtttgcc cgtaccatca ccgtagtgtt ccagcacgcg
    14701 gcggataaag cagcggaaga agcacggttt cgcactctcg atattctgca tgtctccgcg
    14761 tttggcgtgc aggcgctgcg cgttgctatt ccggcactga ttgtctcact gttcgtcagc
    14821 gccgatatgg tgagcaatat gctgagcgcg ataccggaat tcgtgacgcg cggtctgcaa
    14881 attgctggcg gttttatcgt ggtggtcggt tacgccatgg tgctacgtat gatgggcgtg
    14941 aaatatttga tgcctttctt tttcctcggt ttcctcgcag gtggctacct cgatctcagt
    15001 ctgctggcgt tcggtggcgt cggcgtgatc atggccctgg tctacatcca gctaaatcca
    15061 cagtggcgta aagctgaacc tcaactccag gccaccgcct ccaccgccct tgaccaactt
    15121 gacgattaac ggagccatca tggaacagag aaaaattaca cgcagcgatc tggtgagcat
    15181 gtttctgcgc tccaacctgc aacaggcgtc ctttaacttt gaacgtattc acgggctggg
    15241 tttttgctac gacatgatcc ccgccatcaa acgactttac ccattaaaag aggatcaggt
    15301 tgcggcgctc aggcgacacc tggtgttctt caataccacg ccagccgtat gtggcccggt
    15361 catcggcgtc actgccgcca tggaagaggc gcgggccaac ggcgcggaaa ttgatgacgg
    15421 taccattaac ggcatcaaag tcggtctgat gggaccattg gcaggagttg gcgatccact
    15481 ggtctgggga acgctgcgcc cgattaccac cgcgctcggc gcatctctgg cactttcggg
    15541 caacattctc ggcccgctgc tgttcttctt tattttcaat gcggtgcgtc tggcgatgaa
    15601 gtggtatggc ctacagctcg gctttcgcaa aggggtgaat atcgtcagcg atatgggcgg
    15661 gaatgtgctg caaaaactca ccgaaggcgc gtcgattctc ggactgtttg tgatgggcgt
    15721 gctggtcacc aaatggacgt caatcaacgt accgttggtg gtttcacaaa cgcatgccgc
    15781 cgatggctcc accgtcacca tgaccgtgca gaacattctc gaccaacttt gccctggttt
    15841 gctggcgctc ggtctgacgc tgcttatggt tcgtctgctc aacaaaaaaa ttaacccggt
    15901 atggctgatt ttcgccctgt ttggcttagg gattatcggt aatgcgctgg gcttcctgtc
    15961 cagattcttc gccccggcat gactgccggg gccatcgctc aacatgaggt ggtttatgaa
    16021 aacaacagct ctgcgtcttt atggtaaacg tgatttacgc ctggaaacct ttgaccttcc
    16081 tgaaatgcag gaggatgaaa tcctcgcgac ggtggtcact gacagcctgt gcctctcttc
    16141 ctggaaagag gccaatctgg gtgaaaacca taaaaaagta cccgacgatg tggcgaccaa
    16201 ccccatcatc atcggccacg agttttgcgg cgatattctg gccgtgggta aaaagtggca
    16261 gcacaaattc cagccgggtc agcgttatgt gattcaggcc aacctgcaac tccccgaccg
    16321 cccggactgc cccggctact ccttcccgtg ggtgggcggc gaagccacgc atgtagttat
    16381 tcccaacgag gtcatggaac aagattgcct gctggcatac gacggcgaaa cctattttga
    16441 aggctcactg gttgaaccgc tttcctgcgt gattggcgcg ttcaacgcca actatcatct
    16501 tcaggaaggt agttataacc acacgatggg gattcgcccg caagggcgca tgctgatcct
    16561 cggcggcacc ggaccaatgg gactgttggc gattgattat gcgctacatg gacccgttaa
    16621 cccgtcgcag ctcgtcatta ccgataccga caacgataaa ttgagttatg cgcgcaagca
    16681 ctatccatca gaaccgcaaa cactgattca ttatctcaat gccgccgatg cagcatttga
    16741 tacgctaatg gcgctgagtg gcggtcacgg cttcgatgat attttcgtct ttgtgcctaa
    16801 tgaaggactg gtgactctcg cctcttcctt gctggcgaca gatggttgcc tgaatttctt
    16861 cgccggaccg caggataaac atttcagcgc gccaattaat ttctacgatg tgcattatgc
    16921 atttacccac tacgtgggca cgtcaggcgg caataccgac gacatgcgcg cggcggtcaa
    16981 actgattgaa gagaaaaaag tgcaggccgc aaaagtggta acacatattc ttgggctgaa
    17041 tgccgcgggc gaaaccacgc ttgaattgcc tgccgtcggc ggcggcaaaa agctggtgta
    17101 taccgggaaa tacctgccgc tgacgtcact cacgcagatt caggatcaag cactggcggc
    17161 gattctggcg cgtcatcagg ggatctggtc cggtgaggcg gagcaatatc tgctcactca
    17221 tgcagaggca atttcccatg attaatcgcg ctacttaact ctgcatgtcg ctggctggtc
    17281 gccgcggcaa cttcgcgacc tgatgtgcgg ctgattgctg aagcggccca atgtgcacga
    17341 gaaggataaa aaaacatcag cgccttaagg ggcgctcaaa ttggtggcaa agtacacatt
    17401 gtttatgccg gatgcggaat aaagccttat tcggcctaca taatctcgaa aattcaatat
    17461 attgcagtta tctggtaggc ctggtaagcg tagcgcatca ggcaatgctg tgtttgtcat
    17521 cagtctcagc tcccggtgca atacgtgaca tcgtgagcca tgtcagtaag tggttaaatt
    17581 cgcagtggta gcatgccttc cgtgatttct tactaaccgg agaagtaatg gaactgcttt
    17641 tattgagtaa ctcgacgctg ccgggtaaag cctggctgga acatgcactg ccgctaattg
    17701 ctgaacagtt gcagggtcgc cgctcagcgg tgtttatccc tttcgctggc gtaacgcaga
    17761 cctgggatga ttacacagcg aaaacggctg cggttctcgc tccgctgggt gtttctgtca
    17821 ccggtattca tagcgttgtc gatcccgttg ccgcgattga aaatgctgag atcgtgattg
    17881 tcggcggcgg gaatactttc cagttgctga aacagtgccg cgagcgcggg ctgctggcac
    17941 caattactga cgtggttaaa cgtggtgcgc tgtatattgg ctggagcgca ggcgctaacc
    18001 ttgcttgccc aaccattcgt accaccaacg atatgccgat tgtcgatccg caaggtttcg
    18061 atgcgctaaa tctgttcccg ttgcaaatca acccgcactt caccaacgcg ctgccggaag
    18121 gccataaagg tgaaacccgt gagcagcgta ttcgcgaact gctggtcgtc gcgccagaac
    18181 tgacgattat tggtctaccg gaaggtaact ggatcacagt gagtaaaggt cacgctacgc
    18241 tgggtggccc gaacaccacc tatgtgttta aggccggtga agaagcggtt ccgctggaag
    18301 ctggtcaccg tttttaagtg aattgccgga tgcatgatcc ggcatcttgt tcatagtgcc
    18361 tgatgcgacg cttgacgcgt cttatcaggc ctacaggatt cacaatatta atactcgtcc
    18421 cgtccttcat cttcatccga ctgttccagc acactgtaag ccaccgaaca aaacagcgag
    18481 ttcaggcgct gcatatctcc cagtaagcct aaatgtagcg aactggtttc aatgctctgc
    18541 acgttttgct gatgcaggcg atcgacgtgg gcgtgggaat agcggcgatt aagaatgcga
    18601 aagcgatgct tactacggcg caaacgacga gcgctggtga catcgccaga gaagaacacc
    18661 gacattgcca gctttaaatt actgagcaat tgctcataga gcgcatccag ttccttcaac
    18721 ccatcaagcg aaaatgcccg ccgtgctgcc agtgatttgt cagcaatttc gctgcccatg
    18781 cgctcgacga tatcggaggc ctgttcaagg ttgagcgaca tttcaataat ctccgcccag
    18841 cggcgcgact cttcctccgc cagctcttct tttggcatcc gcgccagata cagcttaatg
    18901 gcggtgtaga gaacgttgat atcatctgcc agcttacgca gctctttctc ctgccgtggc
    18961 tcaccgtgca tcactttatt caacccttcc atcatctgtt ccatggcgtc gccaatgcgc
    19021 agggtttcgc gcgcggcgtt cgccagagca agcgtgggcg tatccagcgc gctgacatcc
    19081 agatgtttag gccgtagctg ggtatccagt tccggttcat cgcgaataat cgttttgcaa
    19141 aaccgtgcca tcgggtcaac aaatggcagc atgaccaggc aacgtacaag gttgtagaag
    19201 acgtggaaat agatcaccag ttccgctttt ggcaatgaca gcttccccat tgtctctgcc
    19261 agcaaatgga caaacggcag gataatcagg ctacccacca gcttaaacag cagactaccc
    19321 agcgcgacac ggcgggctgc ggcattggcg gcactgttgt tgagcatcgc cagcaggccg
    19381 gaaccgaggt tagcaccaat caccagacag agcgccacgg ggaaggagat aatgcctgcg
    19441 gcggtcagag ccgcggtcag cagtacagca gcaaggctgg agtagctgat aatggcgaac
    19501 atcgcgccaa tcagcgcatc cagcagaata tcgccggtca gcgaggcaaa gatcacctga
    19561 acgccgtttg cctgggtgat cggcgttacg gcctgcacaa tcaactccag cgccagcaaa
    19621 atcagcccaa gaccaatacc gacgcggccc agttgcccgg cgcgtgactg tttgcgtccg
    19681 aggaaaaaaa tcacgccgat aaaaataagt aacggtgaca gccaggataa gtcgaaggtg
    19741 agaatacgcg ccattagcgc cgtcccgaca tctgccccca gcacaatgac cagagccggt
    19801 gcgagggcta ccagatcctg agcgacgaac gaggtcacca gcatggtggt ggcattactg
    19861 ctctgtacca gtgcggtaac gccgatcccc gcgcaaaagg cgagcggctt tttttcgacg
    19921 ctccggctaa ggacagtacg caaacgcgcg ccgaagacgc gcattacgcc ggttcgaaca
    19981 atatgagtcc cccagaccag cagggcgacg gcagaaagca ggtgaagcag cgttaacacg
    20041 agttcaggtt ctccttatcg ttatatgttc ctgaagcaat tgaatgacca aaggaccgga
    20101 aacacgctga ctggcatctt tactgtgcct tttgacattc gtttttccgg ctaagattat
    20161 tccagcctga ttccagtata agtgtttcat attaataaag agacagggca ctcattgagc
    20221 gccctgtttg ttgtaaggaa aagtgacaga tttgtgaatc agtccgcgtc ataccccaga
    20281 ttcggtgcca gccagcgctc aacatctgag acgctcatac ctttacggcg ggcatagtct
    20341 tcaacctgat cgcgctgaat ttgcgccacg gcgtagtact tgctgtccgg gtggctgaaa
    20401 taccaacccg aaaccgatgc accgggccac atggcgaaag attctgtgag tttcatgccg
    20461 gtatgttttt ccacttccag cagctcccag atggtggctt tttccgtatg ttccgggcag
    20521 gccgggtagc ccggtgccgg acggatgccc tagtagtttt cgcggatcag ttcttcgttg
    20581 ctgaggttct cgttcggcgc atagccccag tagactttac gcacacgctc atggagatac
    20641 tccgcaaagg cttcggctaa acggtcggca agcgctttca ccatgatttt gttgtaatca
    20701 tcgtgctgcg cttcaaaggc atcagccagt gcgtcctctt ccagcccgcc agtcacggca
    20761 aatgcgccga tgtaatctgc tttaccagaa agcttcggcg caacaaagtc agcgagacag
    20821 tagttagcga atcccgtttt ttcggtctgt tgacgcagat ggtggctgac attgatcaca
    20881 tgggtgcgcg tttcgtcacg gtagatttca atgtcatcgc ccacacggtt tgccgggaac
    20941 aggcccacca cgccacgcgg attcagcgtt ttctcggcgc ttaatttatc cagcatgtcg
    21001 ttggcgtctt taaacagccg ctgcgcctca acgcccacca cttcatcttc cagaatgcgc
    21061 ggatacttcc cggccagcga ccaggtcata aagaacggtg tccagtcgat gcaattacgc
    21121 agagtttcga tgctggcttc aacttcctgc acgccgagac ggtgcgccac cggcggcgtg
    21181 taagcctgcc agtcaaaagc gaagtcgtta tcgcgcgccg cttccagcgt gaccggtggt
    21241 gtgcgcggtt tcttgcgccc gtgctgaata cgtacggttt cgtactcctt gcgggtacga
    21301 gcgacaaaat catcgcgctg agtatcggaa agcagcgccg ccaccacacc aacggtgcgc
    21361 gaggcattct gcacatacac cgtcgggccg ctgtagttct gctcaatttt caccgccgtg
    21421 tgcgcttttg aggtggtcgc gccaccgatc agtaacggaa tggtgaagcc ctgacgctcc
    21481 atctctttcg ccacgttaac catctcgtcc agcgacggcg tgataagccc cgaaaggcca
    21541 atcaaatcag cattcacttc tttagcagta cggagaattt tttccgcagg caccatcacg
    21601 ccgagatcga caatttcgta gttgttacat tgcagcacca cgccaacgat atttttaccg
    21661 atatcgtgga cgtcgccctt cacggtggcg atcaccatct tgccgttggt tttgccctgc
    21721 tctttgctgg cttcaataaa cggttcgaga tacgccaccg cctgtttcat gacgcgcgcc
    21781 gatttgacca cctgtggcag gaacattttg ccttcgccaa acagatcgcc gaccacgttc
    21841 atgccgtcca tcaacgggcc ttcaatcact tcaatcgggc gcgtagcctg ctggcgggct
    21901 tcttcggtat cctgctcgat aaactcggta atgcctttga ccagcgagta ttccagacgt
    21961 ttattcactt cccacgagcg ccactccgcc tgctgggcgt tatcggtgtc gtcggttttg
    22021 ctgccgcgat atttctcggc aagctccagt aaacgctcag tgccatcgtc acgacgatta
    22081 agaatcacat cttccaccgc gtcgcgcagt tcagcgggta ggtcgtcgta aatcgccagt
    22141 tgccccgcgt tgacgatccc catatccatg ccattgcgaa tagcgtagta gaggaacact
    22201 gcgtgaatgg cttcgcgcac cggatcgttg ccacggaatg agaaagaaac gttagatacg
    22261 ccgccggaaa tcagcgcgtg cggcagttcg cgtttgatgt cttcacacgc gccgataaag
    22321 tcctgcgcgt agttgttgtg ctcttcaata ccggtggcga ccgcgaagat gttcgggtcg
    22381 aagatgatat cttctggcgg gaagccaacc tcttcggtga ggattttgta cgcccgacgg
    22441 caaatctcga ttttccgtgc gcgagtatcg gcctgtccct gttcatcaaa ggccatcacc
    22501 accactgccg caccgtagcg acgcaacaat ttcgcgtgat ggataaaggc gtcgacgccc
    22561 tctttcatcg agattgagtt aacaatgcct ttgccctgaa tacacttcag ccctttttcg
    22621 ataacgtccc attttgagga gtcgatcata atcggcacgc gagcgatatc cggttcaccg
    22681 gcaatcagat tgagaaaacg caccatcgcc gcttcggcat cgagcatccc ttcatccatg
    22741 ttgatatcga taatctgcgc gccgttttcc acctgctgac gcgccacatc ctgcgcctcg
    22801 ctgtatttct cttctttgat caggcgcttg aacttagcag agccggtgac gttggtgcgt
    22861 tcacccacgt tcacaaacag gctatcgtcg ccaatgttca gcggctccag gccagacaaa
    22921 cggcaggcta cgggaatttc cggcagtttg cgcggcgcta atccttctac tgcacgactc
    22981 atcgctgcaa tatgttgcgg cgtggtcccg cagcaaccac cgacgatatt gagaaagccc
    23041 gcttgcgccc attcacgtat ctgttttgcc atcgtgtcgg cgtcgagatc gtattcacca
    23101 aaggcgttag gcagcccggc gttcgggtgg gcggtgacgt agcattccgc aatccgtgat
    23161 agttcctgca cgtactggcg cagttcatcg ggccccagcg cacagttcag gccaaaggtt
    23221 agcgcttcgg cgtggcgcaa tgagttgtaa aatgcttcgg tggtctgccc ggagagcgtg
    23281 cgcccggagg cgtcggtgat ggtgccggag atcataatcg gcagctcaac gcccagcgct
    23341 tcaaactccg ttttcaccgc aaaaaccgcc gctttggcgt taagggtgtc gaaaacggtt
    23401 tcaatcagga tcagatccgc accaccttcc accagcgctt tggtggattc gcaataggcc
    23461 gccaccagct ggtcaaaagt gatgttgcgg aatgccggat cgttgacgtc cggagaaata
    23521 gacgccgtgc ggttggttgg gccgagaaca ccggcaacgt agcgcggttt ctccggcgtg
    23581 cgcgcggtcc actcgtcagc acaggcgcgc gccagtttcg ccgccgcaaa gttgatttcc
    23641 gccgacaggg attccatctg gtaatccgcc atcgcaatgg tcgtggagtt gaaggtgttg
    23701 gtttcgatga tatccgcgcc cgcttcaaag taggcgttgt ggatagcggc gatcacttcc
    23761 ggtttgctga gcaccagcag gtcgttgttg cctttgagat cgcatggcca gtcggcaaag
    23821 cgttcaccac gaaaatcggc ttcgttcagt cgataactct ggatcatggt gcccataccg
    23881 ccgtccagca ccagaatacg ttcatttaac tgcgcacgca gttgttccac tttgctgctc
    23941 acacttgctc ccgacacgct caacccagat ttaataaaaa ttcaacaaac catactggca
    24001 taaacgcatc tgtggtaaaa gcgaccacca cgcaacatga gatttgttca acattaactc
    24061 atcggatcag ttcagcaact attgcattag ctaacaataa aaatgaaaat gattcccacg
    24121 atgcagaaaa aggagactgt catggtcgca cccattcccg cgaaacgcgg cagaaaaccc
    24181 gccgttgcca ccgcaccagc gactggacag gttcagtctt taacgcgtgg cctgaaatta
    24241 ctggagtgga ttgccgaatc caatggcagt gtggcactca cggaactggc gcaacaagcc
    24301 gggttaccca attccacgac ccaccgcctg ctaaccacaa tgcaacagca gggtttcgtg
    24361 cgtcaggttg gcgaactggg acactgggca atcggcgcac atgcctttat ggtcggcagc
    24421 agctttctcc agagtcgtaa tttgttagca attgttcacc ctatcctgcg caatctaatg
    24481 gaagagtctg gcgaaacggt caatatggag gtgcttgatc aaagcgatca cgaagcgatt
    24541 attatcgacc aggtacagtg tacgcatctg atgcgaatgt ccgcgcctat cggcggtaaa
    24601 ttgccgatgc acgcttccgg tgcaggtaaa gcctttttag cccaactgag cgaagaacag
    24661 gtgacgaagc tgctgcaccg caaagggtta catgcctata cccacgcaac gctggtgtct
    24721 cctgtgcatt taaaagaaga tctcgcccaa acgcgcaaac ggggttattc atttgacgat
    24781 gaggaacatg cgctggggct acgttgcctt gcagcgtgta ttttcgatga gcaccgtgaa
    24841 ccgtttgccg caatttctat ttccggaccg atttcacgta ttaccgatga ccgcgtgacc
    24901 gagtttggcg caatggtgat taaagcggcg aaggaagtga cactggcgta cggtggaatg
    24961 cgctgacttt ttctggcggg cagaggcaat attctgccca tcata
//
LOCUS       DAZPEB010000039        24287 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZPEB010000039 DAZPEB010000000
VERSION     DAZPEB010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24287
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.040"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            174..311
                     /locus_tag="ACHURJ_001436"
                     /pseudo
     CDS             174..311
                     /locus_tag="ACHURJ_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
     gene            complement(419..1654)
                     /locus_tag="ACHURJ_001437"
     CDS             complement(419..1654)
                     /locus_tag="ACHURJ_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFR1280446.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAASDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(1801..1877)
                     /locus_tag="ACHURJ_001438"
     tRNA            complement(1801..1877)
                     /locus_tag="ACHURJ_001438"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1841..1843),aa:Pro,seq:tgg)
     gene            complement(1920..2006)
                     /locus_tag="ACHURJ_001439"
     tRNA            complement(1920..2006)
                     /locus_tag="ACHURJ_001439"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1970..1972),aa:Leu,seq:cag)
     gene            complement(2027..2102)
                     /locus_tag="ACHURJ_001440"
     tRNA            complement(2027..2102)
                     /locus_tag="ACHURJ_001440"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2067..2069),aa:His,seq:gtg)
     gene            complement(2161..2237)
                     /locus_tag="ACHURJ_001441"
     tRNA            complement(2161..2237)
                     /locus_tag="ACHURJ_001441"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2201..2203),aa:Arg,seq:ccg)
     gene            complement(2340..3725)
                     /locus_tag="ACHURJ_001442"
     CDS             complement(2340..3725)
                     /locus_tag="ACHURJ_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR1280447.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAVGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNESGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3916..4656)
                     /gene="wecG"
                     /locus_tag="ACHURJ_001443"
     CDS             complement(3916..4656)
                     /gene="wecG"
                     /locus_tag="ACHURJ_001443"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFR1280448.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4659..6011)
                     /gene="wzyE"
                     /locus_tag="ACHURJ_001444"
     CDS             complement(4659..6011)
                     /gene="wzyE"
                     /locus_tag="ACHURJ_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFR1280449.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTREVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(6008..7087)
                     /gene="rffT"
                     /locus_tag="ACHURJ_001445"
                     /gene_synonym="wecF"
     CDS             complement(6008..7087)
                     /gene="rffT"
                     /locus_tag="ACHURJ_001445"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFR1280450.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRVLAIAAGEVA"
     gene            complement(7084..8334)
                     /gene="wzxE"
                     /locus_tag="ACHURJ_001446"
     CDS             complement(7084..8334)
                     /gene="wzxE"
                     /locus_tag="ACHURJ_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFR1280451.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8336..9466)
                     /gene="rffA"
                     /locus_tag="ACHURJ_001447"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8336..9466)
                     /gene="rffA"
                     /locus_tag="ACHURJ_001447"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFR1280452.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9471..10145)
                     /gene="rffC"
                     /locus_tag="ACHURJ_001448"
                     /gene_synonym="wecD"
     CDS             complement(9471..10145)
                     /gene="rffC"
                     /locus_tag="ACHURJ_001448"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFR1280453.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(10123..11004)
                     /gene="rfbA"
                     /locus_tag="ACHURJ_001449"
     CDS             complement(10123..11004)
                     /gene="rfbA"
                     /locus_tag="ACHURJ_001449"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFR1280454.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLVQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDQERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(11023..12090)
                     /gene="rffG"
                     /locus_tag="ACHURJ_001450"
     CDS             complement(11023..12090)
                     /gene="rffG"
                     /locus_tag="ACHURJ_001450"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFR1280455.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLIYAGNLMSL
                     APVAQSDRFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(12090..13352)
                     /gene="wecC"
                     /locus_tag="ACHURJ_001451"
     CDS             complement(12090..13352)
                     /gene="wecC"
                     /locus_tag="ACHURJ_001451"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFR1280456.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13349..14479)
                     /gene="wecB"
                     /locus_tag="ACHURJ_001452"
     CDS             complement(13349..14479)
                     /gene="wecB"
                     /locus_tag="ACHURJ_001452"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFR1280457.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKAPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELVANYPFIDPNKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14535..15581)
                     /gene="wzzE"
                     /locus_tag="ACHURJ_001453"
     CDS             complement(14535..15581)
                     /gene="wzzE"
                     /locus_tag="ACHURJ_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFR1280458.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15593..16696)
                     /gene="wecA"
                     /locus_tag="ACHURJ_001454"
     CDS             complement(15593..16696)
                     /gene="wecA"
                     /locus_tag="ACHURJ_001454"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFR1280459.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMALGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16936..18195)
                     /gene="rho"
                     /locus_tag="ACHURJ_001455"
     CDS             complement(16936..18195)
                     /gene="rho"
                     /locus_tag="ACHURJ_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFR1280460.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18280..18381)
                     /gene="rhoL"
                     /locus_tag="ACHURJ_001456"
     CDS             complement(18280..18381)
                     /gene="rhoL"
                     /locus_tag="ACHURJ_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFR1280461.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18522..18851)
                     /gene="trxA"
                     /locus_tag="ACHURJ_001457"
     CDS             complement(18522..18851)
                     /gene="trxA"
                     /locus_tag="ACHURJ_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFR1280462.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18982..20247
                     /gene="rhlB"
                     /locus_tag="ACHURJ_001458"
     CDS             18982..20247
                     /gene="rhlB"
                     /locus_tag="ACHURJ_001458"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFR1280463.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEVLEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20381..21865
                     /gene="gppA"
                     /locus_tag="ACHURJ_001459"
     CDS             20381..21865
                     /gene="gppA"
                     /locus_tag="ACHURJ_001459"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFR1280464.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(21912..23933)
                     /gene="rep"
                     /locus_tag="ACHURJ_001460"
     CDS             complement(21912..23933)
                     /gene="rep"
                     /locus_tag="ACHURJ_001460"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFR1280465.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKYYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            24150..>24287
                     /locus_tag="ACHURJ_001461"
     CDS             24150..>24287
                     /locus_tag="ACHURJ_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1280466.1"
                     /translation="MAINFSPKVGEILECNFGYYPVSQNGQFSTTYYDGRIPPEMIKN
                     RL"
BASE COUNT         6108 a         6649 c         6002 g         5528 t
ORIGIN      
        1 gccagaggta ggattgaaaa cgctctcctg attttccaat tcattttctg gataaataaa
       61 taatttattt ttgtcactat tatttatgta atcatcctgt cagggagagg gatctcaatt
      121 atcaatgctt aattacgtca tcattttgat gacatgaaac cggagcattt tgcatggaat
      181 tttcgttttc acccaaactt cttgttgttg ctgtcgccgc cgctcttcct ctcatggcca
      241 gcgcagcaga taccccgtca actgccaccg cacgcaaagg ctttgccgga tacgatcacc
      301 caaaccagta tccacagaac gctgcaattt attgaatttg cacgatcatg taggccggat
      361 aaggcgttta cgccgcatcc ggcaatcaac cgcaggcggc cacaaaatgg ccgctatttt
      421 acttattcac caccagcaaa tgtgcctgca taatgtcact ggccgggcga ccgtgcgcca
      481 gcaaatcagc cattgcttta agatatggcg gtagatggcg gaaataacgc tgatacccgg
      541 cacacaaata attcagcccc ggtttgccgc tggcatcgag catgaagcgg tgtttcgggc
      601 agcctcccca gcacgctttt aacacgttac aactgcgaca ctgcgccggt aattgcttaa
      661 atttatcttc accaaacacc tgctgttgcg gggaatcgat catttctgca attgtttgct
      721 gatgcatatt ccccagccga tattgcggat aaacatagtg atcgcaggcg taaacgtcgc
      781 cgttgtgctc aacaatcacc gagcgcccac aggttggctg atgatggcaa accgcacccg
      841 gcgcaccgac aaaattggca aacgcccatt cgatattcat cacgaaaatc ttgccgacgt
      901 cgcgtttgat ccagtggtca aaaatcgcca ccagaaattc accaaattca tcggggcgca
      961 cagaccattc cgtcagttcc ccctgaatat caccaggcgc atgtagtttc agtccatcgc
     1021 tggcagctgt ttcatcagcc aggcgctcga ccaccggaat aaactggatg aattcgactc
     1081 ccgcatcgca caaaaaatca taaacctgca acggttgctg cgcgctggtg cggttgacgc
     1141 agaccagcac gttatagtcg acatgatgtt tttgcagcag cgtcagggca cgcatcacca
     1201 gcttatgcgt tggtctgcca cctttggtca cgcgatattg attgtggatc tcagccggac
     1261 cgtccagcga taacccaaca agaaaatgat tttctgccag aaatgcacac catttatcat
     1321 cgagcagcac gccgttagtc tggaagctgt tacttatctt cctgccagca ccgtatttcg
     1381 cctgtaactt tacggcacgg cggtaaaaat ccagcccgag taacgttggt tcgccgccct
     1441 gccaggtaaa agccacttcg ttttgcggtt cgctggcagc gatatagtga cggacatacg
     1501 cttccagcgt gtcatcgtcc atatgcgtga ctggcttttc gcggtaaagg gattgttttt
     1561 cgagataaaa acagtagtca cagttcagat tacaatcgga accactcggt ttcgccatca
     1621 catgaaaagc acgcgttgga acctgttgca gcatggtcac tccttgcctg aagtcatcct
     1681 tcaatacgtt aagggcgaaa tcataccgtg gggcaatgag ttattacggg cgtttattgc
     1741 gaagagaggg tgacgaaatg cacagaaaac aaaaaacccc gccgaagcgg ggttcaaaat
     1801 tggtcggcga gagaggattc gaacctccga cccactggtc ccaaaccagt tgcgctacca
     1861 agctgcgcta ctcgccgaaa tactgctttt tgaattttta gttcaattat ttaaagtcgt
     1921 ggtgcgaggg gggggacttg aacccccacg tccgtaagga cactaacacc tgaagctagc
     1981 gcgtctacca attccgccac cttcgcattg tcacaacttc taataatggg gtggctaatg
     2041 ggattcgaac ccacgacaac tggaatcaca atccagggct ctaccaactg agctatagcc
     2101 accactacaa atcttgttac gcggtattac taccaccgca gctcaagcgc cgggactaaa
     2161 tggcgcgccc gacaggattc gaacctgaga cctctgcctc cggagggcag cgctctatcc
     2221 agctgagcta cgggcgctta gcgccgttgc gggggtggat aatacggact tcccacccct
     2281 ctgtctagtc cctttttaaa taaattgcgc gtttggttac ggtttgtgcg ttttgctgct
     2341 tattcctcca gtttatgcgc tttcccgtgg cgattaaggc caaaaacttt ataaatcgcc
     2401 gtcaccgcca gcataaagat gatgccaaca aacagcgaca tacgcgtatc ttcattaaag
     2461 tacatgccga tcaaaacgca aatcaggaat gccattgtta cgtagttggc ccacgggaac
     2521 aggattgagc ggaacggatg gctggcaatc gccgctttat gtgcacgccg aaaacgcagc
     2581 tggcttatca atatcacaaa ccatggcacc atccccggaa gcacgctggc actgtagacg
     2641 tagacaaaca cacgctgcgg attgggaatg atgtagttca ggcatgagcc aattagcaga
     2701 atagcaatag atactgccac acccgcaact ggtacgccgt gacgggaaac tttcgccatc
     2761 gccgccggta actgacggtt tttcgccagt gcgtagagca tacgtccgca actgtacatg
     2821 ccgctgttac agccagagag cgcagccgtc agcaccacaa agttgataat gcccgccgct
     2881 gcggtaatac cgattttggc aaaagtcagt acgaacgggc tgccgttgct gccactttca
     2941 ttccacggga agatggtgac gataacgaaa atcgcgccta cgtagaaaat caggatccgc
     3001 cacagcactt tgcctacggc actgcgcaac gtcacctgcg gattcttcgc ttcaccggca
     3061 gtaatgccaa tcagctccac gccctggtag gacgccacca caatacacag cgcggtcagg
     3121 aaccctttcc agccacccgc aaagaaaccg ccatgctctg tgagattgct aaaaccaatc
     3181 gactgcccgc cattgccaaa gccaaagaaa atcacgccca ggccaatgac aatcatcacg
     3241 ataatcgtgg tgactttgat catcgcgaac cagaactcga tttcgccgta caaccgcacc
     3301 gccgccagat tcgccaacgc caccagcgcc actgcgatca atgcgggtat ccactgcgcc
     3361 atctccggga accagaactg gacataaacg ccaacggcgg tgatttctga gatccccacc
     3421 gccatccaca taaaccaata agaccaggcg gtgagataac caaagaacgg gctcatataa
     3481 cgatgcgcat aaacggcgaa cgaaccggta accggttcga ggaacaacat ttcgcccatt
     3541 gaacgcatga tgaaaaagac gaatagcccg gcgatgatat aggccaacaa tacggatggc
     3601 ccggcccatt tcaaggtact ggcggccccc ataaacaggc cgacgccaat ggtgcccccc
     3661 agggcgatga gttcgatatg tcgagcttcc agcccacgct gtagctctgg tttgttatct
     3721 gccataaatc ctcgtgttgt gtttgcatgc tttccggtgt taccggttat cgttatgggt
     3781 acatcgagtg ttgcgaatgt tttcgtaatt caggagaaat ggcaaataaa gcattaaaaa
     3841 tttgaatgct ttgtgtaata aaaaagcaga caggcgacgg agtgaccact ccgtcgcttt
     3901 acaaagagag gaaaatcaga ggttgccggt gtagtgccag cgtaaataac gcagcaaacg
     3961 aagctgacgc ttaatgcggc tcggctgcga aagcaggcgg tagagccact ccagtcccag
     4021 cgtttgccag attttcggtg cgcgttttac gtgaccggtg aaaacatcgt aagtcccgcc
     4081 aacgcccata tacagcgcat ctggatgcac cagacggcag tcgcgcatga agatctcctg
     4141 ctttggcgat cccatcgcaa cggtgacgat ttgcgctccg ctggcatgaa tgcgttcaaa
     4201 cagcgcctga cgctgctcgg gtttaaaata accatcctga ctgccaacga tattcacatt
     4261 ccactggttg cgcagtttag cttcagtttg cgccagcact tcaggtttac cgcccacaag
     4321 aaataccggc gtcccttctt tgcctgcgcg cgccatcagc tcttcccaga gatcggcacc
     4381 ggcaacgcgg gaaacctgcg cctgcgggta ctttttacgt actgaacgta caacgctgat
     4441 gccatccgca tatttaaatt cggcagcgtt aattaactcc ctgacctcgg cgttatcttc
     4501 aatggtcagc attttttcag cattaatggc aaccagcgtt ccctgcttaa gctgcccgtc
     4561 agcaaacaga taatcgaggg cgtgctgcat atcacgccaa ccaatcaact gtaagccacg
     4621 cagcgtatag gtcggtgccg tggtgttgtt attcattgtt atccttcaac ctgcgtccgg
     4681 agcgatgatt ttgtacgttt atgaatgagt ccggcgcttt caaaaagcca gtacaacagt
     4741 tttgcgatca tcagacatgc gccgaagacc acgataaaaa agactacgcg tgagacaaac
     4801 gaatccagcc cttcacgtgc cagcacgatc atattgaaga tcgccccaaa gcagaaactg
     4861 tgcaatatcg cagccttata gcgattaggc tcgcggttgc ccagctcata cagccagtcg
     4921 aaccatttga tgatcagacc aaccacgatc gccccgagcg ggatgaacaa cgcgccgccc
     4981 atcaccacca gtgagcctat aagcgtaggc gagatcgcca gtccggagtg gttattcagc
     5041 acttccctgg taaagtagtt ggctgagttc agcaccatac tcgggcgacc cggccacagc
     5101 caggaaggga taaagacata gaaatcgcgg acaattggag ccaggccctg gaagtcgatg
     5161 ttgtcgtagt tctgcaacag caacgccaga ttctcccacg gcgagaaggt gtcgcgagtg
     5221 agatagagaa acgtatagaa cgcttcatcg ccgctcacat tcattccata gcgttttagc
     5281 gccagccaga acatgcccac aatccccagc acgcccgccg ccgccagcat ccacaacgaa
     5341 atccagccgc gaataatgcc aataaacagg aagatagcga atgcgatgat gatattggcg
     5401 cgagtgccgc cgacaatcat ataagtcagc aagccaaagg cgaccgtgct gacgaggaaa
     5461 aacagccacg ctttgctatc ctggcgcaga aagtagacca ccagcatcgc cgggatgaaa
     5521 aagtaaaaga agcgttttaa cgccacgccg gagacttcac tggagaagat ctgactgtag
     5581 gagttaagcc ggaacagcaa aaagccgttg tgcatgaaga agatgccgac gcttaccagc
     5641 gcgatgccca tcaggatcac ccacgtaaga ttggtctcca cgcggttcat ggtaaacagc
     5701 ggacggcgcg gtacatcagc aacgcgtttg cgtaggcggg ttttgtaggt gacatagtaa
     5761 accgcgtaga agcagcccgc agaaagcaac gcctgcaaca agatttctgg cggcgcgaca
     5821 ccaacatcaa agcgaaatac cagcacgctg gtcagcggga agccgaagaa aaaggtgagc
     5881 aaaaacagca atgaaaagaa gacattgaag ttaaagcgca cacggcgaaa ctcaaaccaa
     5941 gtcagcgtgg caataaacag cgtgcagagc agccagacca caaacaggcc actgaattgc
     6001 agcagactca tgcgacctcc ccggcggcaa tcgccaacac ccgctgccag ccttgtagat
     6061 agttagggct aaagaaggca atggtgtttt tatccaccga cgccaactgg cgctgcgctt
     6121 cacgcacaat atcctcgttg agatcgtcgg tagtaaacag caccggcaaa tgctgttccg
     6181 tcatatcctg ccagaacgga ttttcccggt taagcacaca aggaatgccc gcctgaatca
     6241 gtaagcaaag cgtaccaatg ccctgctggc gggcaaaaat aaagtaacca agatcgcact
     6301 gacgaagtag cgccagatag gcgtcaaatt ccagtttttc gctcaaaatt tgcagatttt
     6361 cttcgctgaa taactccagc cccgcctgac gaacttcctc aatgtacgct tcgttattag
     6421 gcggatatcc catcggcacc accactttta ccgtatcgcc aaattgctga tgaacggcgc
     6481 gcaaggcagc aatatggtca ttgctgcggt caccggagtt ccccaccaga atggtcattt
     6541 tcccttcacg ttgccgatcg ttcgccatcg tattgagaga agggtccatc cgcgtcggga
     6601 agtacagcag ttcgccccgc acctttggat gcgttttggc aaaaaagctc aaatcaccgc
     6661 gggtggcaaa tacacagccg actcgctttt gcgccaggcg acgtagcggg taaaaaagct
     6721 tatatctcaa gccactggaa agctcataca ggtctgcccc ccagatatgc cagaaaaact
     6781 ggctgggctt aatgccacca ctcagcagag ccagccacag tgtgggattg aactgaccgt
     6841 ggaaaaaaaa tcgctgctga cggttagctt ttgctttcgc gatgaccgct tccgccagcg
     6901 attttttccc agggaaaaat tgcacagaaa gcgccggaca gctatcactt aagccgtcgt
     6961 ccttgccaac aaccataaac tcgcgcgcgt gctcgctcgt cgcggccagc gcgtcattga
     7021 aaaaccgcaa aacggttcgg ttatggtgag ggatatccga tcccagtacg tgaatcagta
     7081 cagtcatgcc cgcctacgcc agagtaaaaa cacgccacaa caaagagaaa aatagacgat
     7141 ataagttgcc atatatgcct gcgccgcgcc cagtgcacca tgcgcaggga ttagccagtg
     7201 ggcaaatacc atcaataaag tgaactggct gacttccgcc agaatataaa accgcagtga
     7261 cgctttggcg atcaccagat aaccaaagac ataagcgccc acttttagca catcacccac
     7321 taactgccag gcaaagagat cgcgcatagc ggtaaattta ttcgacaaca gcaaccagat
     7381 agcaaaatca cgcagcaacc agacggtaaa actcgccgcc gccactgccg gtaagacgaa
     7441 ttttagcgat ttaaccactt cccgggtgat atcgcgcttt tccgttagcc gcgacaacgt
     7501 gggcagcaaa tatacgctga acgatgccgt aatgaattgc aggtaggcat cggaaatact
     7561 gctcacccct tgccagatcc ccacttcatc ccagctatac tgcgccgcca gcagtttacg
     7621 catcatgatg taagcaacag gcaaggtcac cgacgtaatc aacgccatga gcgtaaattt
     7681 gctcaactgc cctgccagac cgttatccca gctgggtttc agatagctta acgggatgac
     7741 accacgtttg atcaacatga tggcggcagg aattaccacc agcgcgggaa tcagcgccag
     7801 acccagcaac gccccttcat aaccgcccaa acggtaactg acgtagtacg cgagaacgcc
     7861 aatcaagctg ccgacaatca gcgataacgc attacctgcg gcatcgcgaa agcctttcat
     7921 cagcgccagt aacaggttgc cccaggcgat ccccatttgc accagcgcca ctaaacgcac
     7981 cagcccctga tagtcggtat taccaaacaa tccctggctg attggcgcag ctgccagcac
     8041 aaaaaccagc gccatcagcg tagagaaacc aagtaccatc gctgatgaag tgccgaccac
     8101 gcggcgcagc tgttgcggat tatcatggta ctgggcaacg tatttggtta caccgttaaa
     8161 gatgccagcc ccggcaagca cgccgagcac ggtaatcaac tggcggaaat ttgccgccag
     8221 cccaagcccc gccggaccaa atgacaccgc cagcaactta ccgaccagta acccggcacc
     8281 aatcttgacc agtgtactgg ccgccgtcca caaggacgct tttgccaacg acatatcagg
     8341 agaagtagtt caacaaagtc gcaattaccg tacgctgatt gacgggcgac aggttgtaga
     8401 acagcggcag gcgcagcagg cgctcgctct ctttggtggt gtagcgatct tcaccgtgga
     8461 actcaccaaa gtgttccccc gcagggcaac cgtgcagcgg aatgtaatga aacaccgcca
     8521 tgatttccgc ttctttcaga aagttaatca acgcgctccg gtcatcaata tcccgcagtt
     8581 taatgtagaa catatgcgcg ttctgcacgc agccatcagg aatcgacggc agctcgatac
     8641 gcccggcttt cgccagaggc gctaacgcat cgtagtaatt ttgccacagc gccagacgtt
     8701 gctggttgat acgatccgct gcttccagtt gcgcccacag gtatgcagct tgcagatcgg
     8761 acatcaaata gctggagcca atatcgcgcc aggtatattt atcgacctga ccacggaaga
     8821 actggctgcg gttagtgccc ttttcacgga tgatctcggc tcgttcgatt aacgctttat
     8881 cgttaatcag cgtcgcgccg ccttcaccac ccgccgtgta gtttttggtt tcatggaagc
     8941 taaagcagcc aatatgacca atggttccca gtgcacgccc tttgtaagtg gacatcacgc
     9001 cctgagcggc atcttccacc acaaacaaat tatgcttttt cgccaacgcc ataatggtgt
     9061 ccatttcgca ggccacaccc gcgtaatgga ccggcacgat aacgcgcgtt ttgtcggtga
     9121 tcgctgcttc aatcaacgtt tcgtcgatgt tcatggtgtc cgggcgaaca tccacaaaga
     9181 cgattttcgc gccacgcagc acaaaggcat tggcggtgga gacaaaggtg tagctcggca
     9241 tgatcacttc atcgccaggc tggatatcga gcagcagcgc cgccatctcc agcgaagcgg
     9301 tgcaggacgg cgtcagtaac actttggcgc tgccaaaacg ttgctccagc cactgctggc
     9361 agcgacgggt aaaaccgcca tcgccacaca gtttgccgct acccattgcc gactgcatat
     9421 agtcgagttc ggttcccacc accggcggtg cgttaaatgg aatcatgtga tcacctgtat
     9481 aaccagtacg cggtgctttc tacattcgca ccactttgta tgtatcgttt aagcgcggcg
     9541 gtgttgccca tttgggtcgc cacccgcaaa gttgttttac cgcgagcata cgcccagttt
     9601 agcgccgttt gcatcagctc agcacctgca ccgcgtccag ccagcaggcc aattcgcgca
     9661 tctgtcgcat tgagttcccg taaagagaca tagccgcgaa tatcgccgga cgccgcacgt
     9721 agaatcagac attgatgatc aaaggtgccg cgcacggcat tttcaatcca ctgtgcataa
     9781 aagcgaccgc tggcgtcagg cgcataccac ggcgcacgaa aacggctttg cgcaaatgcg
     9841 gcgctagcta actgacgtaa tgcgggaata tcggtctctt gtgccacgac agcaccgcta
     9901 tcactgacat tgttcacggg tagcgccaaa tcaacttcac cttctaccag ggagaatccc
     9961 agctgttgca gggcatccag ttcacccgta tttgatgccg caattttggc ctgcacccgt
    10021 gaccacggcg ctaacgcgtc tggcgtcagg agcggtgctt cagacgtaat gcgcacgatg
    10081 gcgctgttaa caccaaagaa ggcgttttcc caggttagtg gctcaatact ggcgcggacg
    10141 ggcacgaagt aactccagca gatattggcc gtagccagtt ttcgctaatg aactggcagc
    10201 acgcttcaca ccctcgtcat cgagccagcc gttacgccag gcgatctctt ccaggcaggc
    10261 aatcttaaag ccctggcgtt tttctactgt ctgcacaaag gtactggctt caatcaggct
    10321 gtcgtgagtg ccagtgtcca gccaggcaaa tccgcgcccg agcagttcaa cggtcaggtt
    10381 gcccgcctcg aggtacatct ggttgatgga ggtaatctcc agttcaccac gctccgacgg
    10441 cttcacctgc tttgcgtact ccacgacttt actgtcgtag aaataaagcc cggtcaccgc
    10501 ccagtttgac ttcggctgtt ttggcttttc ttccagcgag atagcgcgga aattgtcgtc
    10561 aaactccacc acgccaaagc gttcctggtc catcacctga tagccaaata ccgtcgcccc
    10621 ttccgtgcgc gccgcaacat gacgcagctt cggactgaag ccctgaccga agaagatgtt
    10681 atcgcccagc accagacaag aaggttcacc gttgaggaag gtttcaccga tgataaaggc
    10741 ctgcaccaga ccgtccgggc tgggctgttc ggcgtattcc agctggatac cgaactcact
    10801 accatcgccc agcaggcgct ggaaataacc tttatcttcc ggcgtagtga tgatgagaat
    10861 ttcgcggata ccggccaaca tcagcaccga cagcggatag taaatcattg gcttatcgta
    10921 aatcggcaac agttgcttcg atacgccgcg cgtaatcgga tgcaatcggg tgccggaacc
    10981 gcccgccagg ataatacctt tcatgtttgc ctccgctgaa aattagcctt tcagacctaa
    11041 acgctcgccc tgatagctgc cgtcctgcac ctgcttccac cagctttcat tagccagata
    11101 ccactgcacc gttttacgca ttccactttc aaaggtttcc tgtggcagcc agccaagttc
    11161 acgggcaatt ttcgaagcat caatggcata acgcagatca tgccccggac ggtcagcgac
    11221 aaaggtgatc aagtcacgat agtgcaccac gccgtgcggc ttgttcggag ccagttcttc
    11281 cagcagctcg caaatggttt ccacaacatc gagattctta cgctcgttgt gaccaccaat
    11341 attataggtt tcaccgactt tcccggtggt cgccacgcaa tacagcgcgc gggcgtgatc
    11401 ttccacatac agccagtcac ggatttgctg cccgttgcca tataccggca gcggtttacc
    11461 cgccagcgcg ttgaggatca tcagcgggat cagtttttcc ggaaagtggt aaggaccgta
    11521 gttattcgag cagttggtga taagcgtcgg cagaccgtag gtccgcagcc aggcgcgcac
    11581 cagatggtcg ctgctggctt ttgacgcgga ataagggctg ctcggcgcat acggcgtggt
    11641 ttcggtgaag aaatcatccg tcgagtgcag gtcaccatat acttcgtcgg tggagatatg
    11701 atgaaaacgg aacgctgatt ttttatcttc cgtcagcgta ttccagtaag cccgcgccgc
    11761 ttcaagcaat gtataagtcc cgacaatgtt ggtttcaata aacgctgccg ggccgtcaat
    11821 agaacggtca acatggcttt cggctgccag atgcatgaca cagtctggct gatgctcagt
    11881 gaatacgcgt gccagttctg cccgatcgca gatatcaact ttctcaaagg caaagcgatc
    11941 gctttgcgcg accggtgcca gcgacatcag gtttccggcg tagatcagct tatcgaccac
    12001 taccaccgcg tcgctcgttt cgttgatgat ataacgcacc agcgccgagc caataaaccc
    12061 ggcaccacct gttatcagaa tttttctcat cagcgccaga ctcctttggc atcgacgaca
    12121 tactgctgat ggacattgtc gccattgata actttaaact gactatgatc gaccagcatc
    12181 accagcacgt ctgccgttgc cagcgcctcg tcaagctgcg ccagagtaca aagcccggtc
    12241 agttttttcg gcaactggtg gatgttaggc tcaacaacca gagtttcgcc gctatgccac
    12301 tgggcgatca gttcagcgat ttccatcgcc gggctttcgc gcaggtcatc aatattcggt
    12361 ttaaacgcca gaccaaagca ggcgattttc agttcactgg cgcgtttatc ggtagccgcc
    12421 aggcaatcag ccaccgccgc tttcacctga tcgataaccc agaacggttt gtgatcgttc
    12481 acttcgcgcg cggtacggat aagccgcgcc tgctgggggt tctgtgccac gataaaccac
    12541 ggatcaacag caatgcagtg accgcccacg ccagggccag gctgaagaat attaacgcga
    12601 gggtgacgat tcgccaggcg aatcagttcc cagacgttaa tcccctgatc ggcacaaatc
    12661 agcgacaatt cattagcaaa agcgatattc acatcgcgga agctgttttc ggtgagctta
    12721 cacatttccg ccgtccgcga gttagtgacg acacactcac cttcgaggaa aattttgtac
    12781 agttcgctgg cgcgggtcga acaaaccggc gtcataccac caatcacgcg atcgttttta
    12841 atcagctcga ccataacctg gcccggtaac acgcgttccg ggcagtaagc aatgttgacg
    12901 tccgcctgct cgcccacctg ctgtgggaaa gtgagatccg gacgcatctc tgctaaccat
    12961 tctgccatct tctcggttga ccccaccggc gaggtggatt caaggatcac cagcgcgcct
    13021 tttttcagca ctggcgcaat ggagcgagca gccgattcaa cgtaggtcat atctggctca
    13081 tgatcgcctt taaacggcgt gggtacagca atcagccagg catccgcttc aactggcgtc
    13141 gtgctcgctc gtaaaaaacc gccttctacg gcagttttta ctacgctcgc caaatcaggc
    13201 tcgacgatat ggatttcgcc acgattgatg gtatcaaccg cgtgttggtt gatatcgaca
    13261 ccaatcacct gtttttgccg tgaggcaaac gctgctgctg taggcagccc gatgtaaccc
    13321 agtccgataa cagaaatggt cgcaaaactc atagtgatat ccgattattt tttaacgctt
    13381 ccagaatgcg agagcatgcc tgaccatcac catacgggtt atgggcgcgg ctcatagttt
    13441 gatattcgtt ttcgtctttt aaaagacgcg tcacttcctc gacaattcgc tgcttatccg
    13501 tgcctaccag acgcaccgta cccgccgtca ccgcttccgg acgctcagtg gtatcgcgca
    13561 tcaccagcac cggtttcccc agcgaaggcg cttcttcctg aatgccgcct gagtcggtca
    13621 aaatcagcca ggcgtggttc atcaaccaga caaacggtaa atactcctgg ggatcgatca
    13681 gaatgacatt tttcacatgc cccagaatgc gattgaccgg ctctctgacg ttcgggttga
    13741 gatgcaccgg atagacaatc tggatgtcct ggtgcgtggt ggcaatgtct gccaacgcgt
    13801 ggcagatttc ttcaaaacca cgaccgaaac tctcacgcct gtgaccggtc accagaatca
    13861 tctttttatt ggggtcgata aacgggtaat ttgccaccag ttctgaacgc agcgtgtcgc
    13921 tgctcatcac ctgatcacgc acccataaca gtgcatcaat gactgtatta ccagtaatga
    13981 agattcggct atccgcaacg ttttcacgca gcaagttttg ccgggaagtt tcggttggag
    14041 agaagtgata catcgccaga tgcccggtca atgtacggtt agcctcttcc ggccacggcg
    14101 aatagagatc gcccgtgcgt aaaccagcct caacgtgacc aacaggaata cgctgataaa
    14161 acgccgccag gctggttgcc agcgtcgttg ttgtgtcgcc gtgaaccagc acgacgtctg
    14221 gtttgaactc ggcaagaata ggttttagcc cttccagaat ccgacaggtt atctctgtca
    14281 ggccctgtcc tggctgcatt atgttgagat cgtagtcagg tacaatggaa aagagtttca
    14341 gcacctgatc gagcatctcc cgatgctgcg cagtgacgca aactttagcc tcaaaaaaag
    14401 gagcttttgc caacgcatgc accaacggag ccatcttgat ggcttccggg cgcgtaccaa
    14461 atacagtcag tactttcaca tcgattctct tcgaataagc ggcgagcgcc tttgcgctcg
    14521 ccgcagcagt gttgctattt cgagcaacgg cgggttaatg cgacaccagc cccgatcagc
    14581 cccccgacaa tgccccacat aatcatcagg aaggcacgac gtgggctatc gcgttttacc
    14641 ggttcttccg gcgtacgcaa atagcgatag gtctgaaaac gcggatccag ggttggacca
    14701 acattcaggg tgtttaacat ggcccgattc tgatcatagt cgagatcaaa ggccggaccg
    14761 acggcctgta aattttccag tcgagcctgg agcattggac gcccaagcag gaacatttct
    14821 gaatcaggta attcctcggc aggcacatct gtcgcactgc gcgaaatatt atgctgctca
    14881 gcaattttca gcgcctgctc aatgctgttc atccggcggt cgtagatggc tttcgccacc
    14941 tcttcctgac gcttcacctg agctttcatc tggatggtgc gtgccgccca tgcgcctttc
    15001 agctcatcat tcagatggct ggctgcacgc tggctggcaa aagcaacata ctgacgtaac
    15061 aggttattag cgtcaggcgc ggtttcggca ataagcttca cgctgtcatt gaccgcgcgg
    15121 gtaaagtctc cggggataaa ctggatgttg ttaatcattt catccagcaa cgccgcatcg
    15181 gctttgctgt tgcccaccat ccgctgtttg taatagtcgg tttgcagcca gaactctctg
    15241 cgggtatccc acgaggccag ttgcataaca aactctttat aggcttcgtc catgaccgat
    15301 ggttgatcgg cagaagccat gtttgaacgg acatccaggt tacgcaaaaa ttgctgctgc
    15361 gagtaatatc cccccagcat attcaccgtt ggacgatcgg taatcgccgt cgagctccac
    15421 tcctgacgag caaaaaaagt atacgccagc gcgattaacg caaacgccag ccccatgcca
    15481 ataatccata gcttcccagc ccacaaggta cgaaacaacc cacgaatatc cagttcattt
    15541 tcagcgtctt cggccggttt cccaggcatt ggttgtgtca tcacatcctc atttatttgg
    15601 ttaaattggg gctgccacca cgatttctac gcagtctgcg ttttacgcgc ttaataaagc
    15661 gagcaacttt ccaggcacgc ttaatgcaat atccatagag gaagaatgct agcaaaaaga
    15721 gcaccagcat gacccactcc gggacaaaat gagaatattc tgccagcacg ccaatggaag
    15781 cgagcagtgc tgcggcaagg gtaatcagca caaacgcctg acgggaagta aacccggcac
    15841 gcatgatcaa atggtgaata tgctgacggt caggagagaa tgggctcatg cctttacgca
    15901 ggcgacggta cataatcgcc accatatcca ttagcggaat ggcgattatc cacaaagcgg
    15961 taaccgggct gatgggatgg gttttgccct gggtcgtttc gagcaggatc cagataacgg
    16021 taaaaccaat cagcgtactg cccgcatcac ccataaagac tttgtagcgg cgacccagga
    16081 taccaaggtt aagcatgatg tatggcagga tggcggcgat catcgcaaag caccagattg
    16141 cgaggctggt ttgcccgtcg aaccacaaaa tcataccgat tgctgcaaac gagacgcagg
    16201 acaacccgcc cagcaagcca tcaatgccat caaccatgtt gaacgcatta atggccgccc
    16261 agacggcaaa tagcgtcagg aagtaaccaa acggtccgag cgccatctcc caggagccaa
    16321 agatataacc caggctactg agataaagtt tgccgaacac catcataaca atgccaacag
    16381 cggcctgtat ggtggcacgg atttttacgc tgatatcaaa acggtcatcc agcgcgccaa
    16441 tgaaaacaag cacaccggca caagcgagat agagagatgc atgcggaata tagtaatcga
    16501 caattccgaa cgtgaagcaa atccctgcgt aaaccgaaat ccccccaacg agaggtatca
    16561 atccctggtg acgtttgcgg aagtttggtt tatccactaa accgactttt tttgccacct
    16621 tacgggcaaa aaacagaaac agtgtcgtga ataaaaaaat actgatgaga tcagtactca
    16681 ctgtcagtaa attcacaatg catgctctca gagaaaatta ttagcagaag tataaccacg
    16741 aagaccttta ttcagaaggg aaagtccgtt ccgaacccac cagtcctgtg caaaaattaa
    16801 ccatttgata tgcaaattgc ataacatgcc agcaaattcc aggaattttc ctaacatcgc
    16861 cagcgcggca ttaagattac agatataaaa gcaaaacgcc acgtaaacac gtggcgtttt
    16921 tggcataaga caaatttatg agcgtttcat catttcgaag aaatcgtcat tggtcttggt
    16981 catggccagt ttattaatga ggaattccat tgcatcgatt tcgcccatcg ggtgaatgat
    17041 tttgcgcagg atccacattt tctgcagttc ttcctgagtc gtgagcagct cttctttacg
    17101 ggtaccggaa cggttgtagt cgatagccgg gaagacgcgt ttttcagcga tcttacgaga
    17161 gaggtgcagt tccatgttgc ctgtaccttt aaactcttcg taaataactt cgtccatttt
    17221 agaaccggta tcgataagcg ccgtcgcgat aatggtcagg ctgccgccct cttccacgtt
    17281 acgcgccgca ccgaagaagc gtttcggacg atgaagggca ttggcgtcca caccaccggt
    17341 caacacttta cctgacgccg gaacaacagt gttgtaagcg cgcgccagac gagtgatgga
    17401 gtcgagcaga atgataacgt ctttcttgtg ctcaaccagg cgtttggcct tctcgatcac
    17461 catttccgca acctgaacgt ggcgagatgc gggttcgtca aaggtagaag caacaacttc
    17521 accttttacc agacgctgca tctcggttac ttcttccgga cgttcgtcga tcagcagaac
    17581 catcagcaca caatccgggt ggttgtaagc aatgctctga gcaatgttct gcagcagcat
    17641 agttttaccg gctttcggcg gtgccacaat cagaccacgc tgaccacgac cgataggtga
    17701 tgccagatcc agtacgcgag cggttaaatc ttccgtagaa ccgttaccac gttccatacg
    17761 cagacgagag tttgcgtgca gcggggttaa gttctcaaag aggattttgt tgcgggcgtt
    17821 ttcaggtttg tcgaagttaa cttcgttaac tttcagcagc gcaaaatagc gttcaccttc
    17881 tttcggcggg cgaatcttac cagagatggt atcaccagtg cggaggttga aacggcggat
    17941 ttggctaggg gaaacgtaga tgtcatcagg accggcgagg taggagctgt ctgcggaacg
    18001 gaggaaacca aatccatcct gcaatatctc cagtacgcca tcaccaaaga tatcttcgcc
    18061 actctttgcg tgctgcttca ggatggcaaa aataatgtcc tgcttacgca tacgagccag
    18121 gttttccagc cccatatttt cgccgagagt gatcagctca gaaaccggcg tattctttaa
    18181 ttcggtaaga ttcatagtgg tgtgagttct taaacttggg gtaaatctcg aacttaatgt
    18241 tgtgaatggt atggcaggat catccatgcc tgtttacggt catcgactca tgtcttttcg
    18301 ctgcctggtc acaggagagt acgcagaact gaaacgacaa gacggattga gtgacgagcc
    18361 agaaatctgt tcacttcgca tttaagataa aacgggaagc gttggaaatt acaagattca
    18421 aacttaataa ggtatgttta atacgaagtc aacactaagt tagcatgact cacgccgggc
    18481 gtccagtttt taacgacggg gcacccgaac atgaaattcc cttacgccag gttagcgtcg
    18541 aggaactctt tcaactgacc tttagacagt gcacccactt tggttgccgc cacttcaccg
    18601 tttttgaaca gcagcagagt cgggatacca cggatgccat atttcggcgc agtgccaggg
    18661 ttttgatcga tgttcagttt tgcaacggtc agtttgccct gatattcgtc agcgatttca
    18721 tccagaatcg gggcgatcat tttgcacgga ccgcaccact ctgcccagaa atcgacgagg
    18781 atcgccccgt ccgctttgag tacatccgtg tcaaaactgt cgtcagtcag gtgaataatt
    18841 ttatcgctca tatataactc cacaggaata agcctggcgt gttggtttcg ttgttggtgt
    18901 aacattaacc aactaaaggt tgactttatt tcaccggata cgctttcgta aagcaatagt
    18961 aagctgatat tctaccacac tatgagcaaa acacatttaa cagaacagaa gttttccgac
    19021 ttcgccctgc atccgaaggt tgtagaagtc cttgaaaaaa aagggtttca taactgtacg
    19081 cccattcagg cactggccct tccgctgacg ctggcgggtc gtgacgtagc cgggcaggcg
    19141 caaaccggta ccgggaaaac gatggcgttt cttacgtcaa cgtttcatta tcttctctct
    19201 catcctgcga ttgccgatcg caaggtgaat caaccacgtg ccttaattat ggcaccgacg
    19261 cgtgaacttg ccgtgcagat ccatgccgac gcagaaccgc tggcggaagc taccggcctg
    19321 aagctgggtc tggcttacgg tggtgatggc tacgacaaac agctgaaagt gctggaaagc
    19381 ggcgttgaca ttctgattgg cactacgggg cgtttaattg actacgccaa gcagaaccac
    19441 attaacctcg gtgccattca ggtggtggta ctggacgaag ccgatcgcat gtacgatctg
    19501 ggctttatta aagatatccg ctggctgttc cgccgtatgc cgcctgcaaa ccagcgcctc
    19561 aacatgctgt tctccgccac gctttcgtac cgggtacgtg aactggcgtt cgagcagatg
    19621 aacaatgccg aatatattga agtggaaccg gaacagaaaa cgggccaccg tattaaagaa
    19681 gagcttttct acccttctaa cgaagaaaaa atgcgtttgc tgcaaacgct gatcgaagaa
    19741 gagtggccag acagagcgat tattttcgcc aataccaaac accgttgtga agagatctgg
    19801 ggccacctgg cggcagatgg tcatcgtgtc ggtttgttga caggcgatgt cgcgcagaaa
    19861 aaacgtctgc gtattcttga tgaatttacc cgtggcgatc tggatattct ggttgccacc
    19921 gacgttgccg cgcgtggttt gcatattccg gcagtgacgc acgtctttaa ctacgattta
    19981 cccgatgact gtgaagatta cgttcaccgt attggtcgta caggtcgcgc aggcgcaagc
    20041 ggtcactcta tcagcctagc gtgtgaagag tatgcattga atttgcctgc tattgagacc
    20101 tatattggtc actcaattcc ggtaagcaaa tacaatccgg acgcattgat gaccgatctg
    20161 ccaaaaccgc tgcgcctcac gcgcccacgt acaggcaatg gtccgcgtcg tactggcgct
    20221 ccgcgtaatc gtcgtcgttc aggttaaata aaatgtcata ctcaaaatca ttcaaactgt
    20281 atcaagatgg caagagaatg aatcccgatg agcatactgt agtaagtgat tcaggtgaaa
    20341 gaatgcagcc aacacagaga cagattgaag gatgaagagt atgggttcca cctcgtcgct
    20401 gtatgcagcc attgatctcg gttcgaatag ttttcatatg ctggttgtgc gcgaggtggc
    20461 tggaagcatc cagacgctga cgcgaataaa acgcaaagtg cgtctggctg ctggcctgaa
    20521 tagcgaaaat gccctgtcta atgaagcaat ggagcgcggt tggcaatgtc tgcgcctgtt
    20581 tgctgaacgt ctgcaagata tccctccctc gcaaattcgc gttgtcgcta cggcgacatt
    20641 acgtcttgcc gtcaatgcgg gtgattttat tgccaaagcg caggaaatcc tcggttgtcc
    20701 ggtacaggtg atcagcggtg aagaggaagc acgtctgatt tatcagggcg ttgctcacac
    20761 aactggcggt gccgatcagc gcctggtggt ggatataggc ggtgccagta ctgaactggt
    20821 aaccggcacg ggtgcacaaa ccacctcgtt gttcagcctg tcgatgggct gcgtcacctg
    20881 gctggaacgc tattttgccg atcgtaatct ggggcaggaa aattttgatg ctgcagaaaa
    20941 agcggcacgc gaagtgttac gtccggttgc cgatgaatta cggtatcacg gctggaaagt
    21001 gtgcgttggt gcttccggca ccgtgcaggc gttacaggaa atcatgatgg cacaggggat
    21061 ggatgaacgc attaccctgg aaaagttgca gcaattgaaa cagcgagcca ttcattgcgg
    21121 tcggctggag gaactggaga ttgacgggct gacgctggaa cgtgcgttag tgttcccgag
    21181 tggtctggcg atcctgatcg ccatttttac cgaactgaat attcagtgta tgaccctggc
    21241 gggcggtgcg ctgcgtgaag gcctggtcta cggcatgttg catctggccg tcgagcagga
    21301 tattcgcagc cgtacgctgc gtaatattca gcgccgcttt atgatcgata ttgatcaggc
    21361 gcagcgcgta gccaaagttg cggctaactt cttcgatcag gtggaaaagg aatggcatct
    21421 tgaagcaata agccgcgatt tgctcatcag cgcctgtcag cttcatgaaa tcggcctgag
    21481 cgttgacttt aaacaagcgc cgcaacacgc tgcttatctg gtgcgtaatc tggatcttcc
    21541 cggttttacc cccgcacaga aaaaattgct ggcgacgcta ctgctcaacc agactaatcc
    21601 ggtcgatctc tcatcgctgc atcagcaaaa tgccgtaccg ccgcgcgtcg cagaacaact
    21661 ctgccgttta ctccgcctgg ccatcatttt tgccagccgt cgccgtgacg atctcgtgcc
    21721 agagatgaca ttacaggcta accatgaact gttgaccttg acgcttccgc aaggttggct
    21781 aacccaacat ccgctgggta aagagattat tgctcaggaa agccagtggc agagctatgt
    21841 ccactggccg ctggaagtgc attaatgagt aagtgccgga tgcgatgctg acgcatcttt
    21901 tccggccttg attatttccc tcgttttgcc gccatcatcg ctttcagatt cgccagatgg
    21961 ctttgccctt tctgcatccg ttcttcggcg ctgaccactt tgcgctcctg ttcccaaatc
    22021 agatcatcct gcggcagctc cagcaaaaag cggctcggct ccgggcgcac cagctcgccg
    22081 tactgacggc gttctttaca cagcgtaaag gtcaattcct tctgggcgcg ggtaatgccg
    22141 acataggcca gccgccgctc ctcatcgata ttatcttcat cgatgctgct ctggtgcggc
    22201 aaaaaccctt cttccatacc gaccatgtag acataaggaa actccagccc ttttgacgcg
    22261 tgcagagtca tcagttgcac ctgatccagc tcttcttcac tctcaccacg ctccatcatg
    22321 tcgcgcaaag taaagcgcgt caccacctgg gtgagcgtca tcggctcatc cagttcgctg
    22381 ccttccagca tctccgtcat ccagctaaac agttggttga cgttcttcat gcgcatttcg
    22441 gcggctttcg ggctgggcga tgtttcgtac agccaggatt cataatccat gccgtggatc
    22501 agatcgcgca ccgcggcaat cggctcccgc tccgccagac gctggatttc tgccaaccag
    22561 tgagtaaagc gggtcaatgc ttcataacca cgtccgctaa gcgtctgact caggcccata
    22621 tcaaagctgg cggtaaacat gcttttattg cgcgtcatcg cccactcacc cagctttttc
    22681 agcgtcgccg ggccaatctc tctcttcggc gtgttaacga tacgcagaaa tgcgctgtca
    22741 tcgtccgggt tagtcagcac gcgcagataa gccagcaagt ccttgatttc aggacgagag
    22801 aaaaacgacg taccaccaga aattttgtac gggatgcggt tttgcatcag gaatttttca
    22861 aacactcgcg actgatggtt accgcgataa agaatagcgt aatatttgta ctgcgtttta
    22921 ttgacgaagt gatgcgcaat cagttcacca gtgacgcgct cggcttcatg ttcttcgtta
    22981 ttcgcgctta aaactttaag ctctgtgccg taacccagtt cggagaacag acgcttctca
    23041 aagacgtgcg ggttattggc gatcaggatg ttcgccgctt tcaaaatacg tccggaagag
    23101 cggtagttct gctcaagctt aatcaccttc agcgccggaa aatcctgact cagcagcacc
    23161 aggttttgtg gacgtgcgcc gcgccaggag tagatcgact ggtcgtcatc gcccaccacg
    23221 gtaaagcgcg cgcggctgcc caccagcagt ttcaccagtt catactggct ggtgttggtg
    23281 tcctgatact catccaccag cagatagcga attttgttct gccagcgctc acggacttct
    23341 tcattgcgtt gcagcagcaa cgtcggcaat aaaatcagat catcgaagtc gagaacattg
    23401 cacgctttca ggtgtgcatc atacagccca taacaatggg caaaaatacg gtcccgctca
    23461 ccaatcgcac tggctgccgc ctgggacggt gttttgagat cgttcttcca gttagagatg
    23521 gtcgaaatca actgttgcag gagaactttg tcatcttcaa tcagcccttc ggtcaactct
    23581 ttgagcaacg ccagctgatc ggtatcgtca aacagcgaaa agttcgcctt catccccagc
    23641 gccgcatact cacgcttgat gatatccagc cccagcgtgt ggaaggttga gatcatcagc
    23701 ccacgcgcct ctttgcgacc cagcgtctgt ccgacacgct ctttcatctc gcgcgccgcc
    23761 ttattggtaa aggtcaccgc cgcgatatgt cgcgcctgat aaccgcaacc gcggatcaga
    23821 tgggcgattt tattggtgat cacacgagtt ttaccggaac ccgcgcccgc cagcaccagg
    23881 caggggccgg taacgaattc gacagcttgt tgttggccgg ggtttagacg cataggtgta
    23941 ttgctcaatc ttcgaacggg gggaggattg tagcagaatc gcatttgaat gtgaccgcgt
    24001 gtaagccgct aaccggttgg ctgcggatgt ttaaatcaga gagggaagac cgaaaccgga
    24061 gttcatcgtg aatccattag acttagaaaa tatcgggtcg taacgttgtg ttattacttt
    24121 ttcagagttt actaaggaga ggtgtactcg tggcgattaa tttcagtcct aaggttggtg
    24181 aaatactgga atgcaacttt ggttactatc ctgttagcca gaatggacag ttcagcacca
    24241 cgtattacga tggccgcatt cctcctgaaa tgataaaaaa caggtta
//
LOCUS       DAZPEB010000040        24226 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZPEB010000040 DAZPEB010000000
VERSION     DAZPEB010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24226
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.041"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(83..964)
                     /gene="yddG"
                     /locus_tag="ACHURJ_001462"
     CDS             complement(83..964)
                     /gene="yddG"
                     /locus_tag="ACHURJ_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFR1280467.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEAGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTQQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            1196..4243
                     /gene="fdnG"
                     /locus_tag="ACHURJ_001463"
     CDS             1196..4243
                     /gene="fdnG"
                     /locus_tag="ACHURJ_001463"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:1781..1783,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFR1280468.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIAVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLAGQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGERVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            4256..5140
                     /gene="fdnH"
                     /locus_tag="ACHURJ_001464"
     CDS             4256..5140
                     /gene="fdnH"
                     /locus_tag="ACHURJ_001464"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFR1280469.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            5133..5786
                     /gene="fdnI"
                     /locus_tag="ACHURJ_001465"
     CDS             5133..5786
                     /gene="fdnI"
                     /locus_tag="ACHURJ_001465"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFR1280470.1"
                     /translation="MSKSKMIVRTKFIDRVCHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(5837..6121)
                     /locus_tag="ACHURJ_001466"
     CDS             complement(5837..6121)
                     /locus_tag="ACHURJ_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFR1280471.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(6267..7277)
                     /gene="adhP"
                     /locus_tag="ACHURJ_001467"
                     /pseudo
     CDS             complement(6267..7277)
                     /gene="adhP"
                     /locus_tag="ACHURJ_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
     gene            complement(7411..9108)
                     /gene="maeA"
                     /locus_tag="ACHURJ_001468"
     CDS             complement(7411..9108)
                     /gene="maeA"
                     /locus_tag="ACHURJ_001468"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFR1280472.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     IDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            complement(9265..9402)
                     /gene="sra"
                     /locus_tag="ACHURJ_001469"
     CDS             complement(9265..9402)
                     /gene="sra"
                     /locus_tag="ACHURJ_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFR1280473.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            complement(9504..9719)
                     /gene="bdm"
                     /locus_tag="ACHURJ_001470"
     CDS             complement(9504..9719)
                     /gene="bdm"
                     /locus_tag="ACHURJ_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFR1280474.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            10065..10496
                     /gene="osmC"
                     /locus_tag="ACHURJ_001471"
     CDS             10065..10496
                     /gene="osmC"
                     /locus_tag="ACHURJ_001471"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFR1280475.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSFMLGEAGFTPTSIDTIADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            complement(10552..11478)
                     /locus_tag="ACHURJ_001472"
     CDS             complement(10552..11478)
                     /locus_tag="ACHURJ_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFR1280476.1"
                     /translation="MSDTLLTLRGVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALVEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGDAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     YERCPLATSGCEVRQSLAIREDGRELRCWRAL"
     gene            complement(11471..12457)
                     /locus_tag="ACHURJ_001473"
     CDS             complement(11471..12457)
                     /locus_tag="ACHURJ_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1280477.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIVLLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVRADVIHHPRHPYTIGLLQCAPEHGVPRQLLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            complement(12454..13350)
                     /gene="ddpC"
                     /locus_tag="ACHURJ_001474"
     CDS             complement(12454..13350)
                     /gene="ddpC"
                     /locus_tag="ACHURJ_001474"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFR1280478.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLIWMLKGSPLTVTGAVIIVLML
                     LMMFFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            complement(13347..14369)
                     /locus_tag="ACHURJ_001475"
     CDS             complement(13347..14369)
                     /locus_tag="ACHURJ_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1280479.1"
                     /translation="MTFWSILRQRCWGLVLVMAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            complement(14371..15921)
                     /locus_tag="ACHURJ_001476"
     CDS             complement(14371..15921)
                     /locus_tag="ACHURJ_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFR1280480.1"
                     /translation="MKRSIPFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLA
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDKDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            complement(15935..16516)
                     /gene="ddpX"
                     /locus_tag="ACHURJ_001477"
     CDS             complement(15935..16516)
                     /gene="ddpX"
                     /locus_tag="ACHURJ_001477"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HFR1280481.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFTCFISPGTQHVS"
     gene            complement(16774..19173)
                     /gene="dosP"
                     /locus_tag="ACHURJ_001478"
                     /gene_synonym="pdeO"
     CDS             complement(16774..19173)
                     /gene="dosP"
                     /locus_tag="ACHURJ_001478"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HFR1280482.1"
                     /translation="MKLTDAENAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFRGKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNHDYL
                     LSTAHNAIDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMHAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            complement(19198..20579)
                     /gene="dosC"
                     /locus_tag="ACHURJ_001479"
                     /pseudo
     CDS             complement(19198..20579)
                     /gene="dosC"
                     /locus_tag="ACHURJ_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
     gene            complement(20951..22270)
                     /locus_tag="ACHURJ_001480"
     CDS             complement(20951..22270)
                     /locus_tag="ACHURJ_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFR1280483.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIT
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            complement(22401..23936)
                     /gene="gadC"
                     /locus_tag="ACHURJ_001481"
     CDS             complement(22401..23936)
                     /gene="gadC"
                     /locus_tag="ACHURJ_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFR1280484.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            complement(24092..>24226)
                     /locus_tag="ACHURJ_001482"
     CDS             complement(24092..>24226)
                     /locus_tag="ACHURJ_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1280485.1"
                     /translation="IMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT
                     "
BASE COUNT         6014 a         6135 c         6135 g         5942 t
ORIGIN      
        1 aattactggt atttgaaatt tattttttaa tattgtcgga atttatctga ttaactgccg
       61 ggccgtagac ccggcaatta ttttaaccac gacgtgtcgc cagccagcag agcagggaac
      121 cgccgcagac cattagcgcg ccttgccaga atgagaacga cagcggggcg ctgagcagca
      181 cggctgcaag cgctgaggaa agtacgggcg taaaatacga gcctaccgcc attatggtga
      241 cattgccatg caatataccg acattccatg cagcataagc aaatcctaag gtaaatgccg
      301 cagagatgag tttaatcatg acgggcgtgc taaataccat ttctggttgt tgcgtaagaa
      361 aatagtaaac ccacagactt gctcccgtta gcaggacaaa aacggtaatt ccgttaaatc
      421 cgcgtgcgta tttattcgtt actgtgcaat aggctgccca gataaacgca ccaataaacg
      481 ccaggaaata actcaatggg ctggtggtga tattattgat gatttcatca taatgtaacc
      541 cattctcacc gcctaacacc cagcagacgc cgaccagggc taataataat cctggtacaa
      601 tcagccagtt ggttttctga ccattaaaca gaatggcaaa gagaattgtc aggctgggcc
      661 acagatagtt caccataccc gcttcaatcg cctgatgacg ggtcgccgca taccctaagg
      721 aaagcgccag acagatttca tagctgacga ataacagact cccggcgagt aaatagcctt
      781 tcggaatctg ccgaatacgc ggaaatccaa ccgtgaagat taacagcagc ccgcttaatg
      841 aatagatagc agctgcgccg ccgaccgggc cgagcccctc gctgacaccg cgaatcaatc
      901 ctaccatcgt gctccacagg acgatcgcta ttagccctat gagcgttgct ttttgtcgtg
      961 tcatgctcgc tgttttgtct ctcttgccgt taaaaattaa gctgaatttt atagcatttt
     1021 tttaactggc ctgtcaggca gtggtgcgtt tttctaccgc tattgaggta ggtcaatttg
     1081 cgcaggcgga ttattttgtg gcaaacacgt gttctttttg atttcgcgca aaaagattca
     1141 gaattttact gttagtttcc tcgcgcagta atatccctga aaaaagagga aagcaatgga
     1201 cgtcagtcgc agacaatttt ttaaaatctg cgcgggcggt atggctggaa caacggtagc
     1261 ggcattgggc tttgccccta agcaagcact ggctcaggcg cgaaactaca aattattacg
     1321 cgctaaagag atccgtaaca cctgcacata ctgttccgta ggttgcgggc tattgatgta
     1381 tagcctgggt gatggcgcga aaaacgccag agaagcgatt tatcacattg aaggtgaccc
     1441 ggatcatccg gtaagccgtg gtgcgctgtg cccaaaaggg gccggtttgc tggattacgt
     1501 caacagcgaa aaccgtctgc gctacccgga atatcgtgcg ccaggttctg acaaatggca
     1561 gcgcattagc tgggaagaag cattctcccg tattgcaaag ctgatgaaag ctgaccgtga
     1621 cgctaacttt attgaaaaga acgagcaggg cgtaacggta aaccgttggc tttctaccgg
     1681 tatgctgtgt gcctccggtg ccagcaacga aaccgggatg ctgacacaga aatttgcccg
     1741 ctccctcggg atgctggcgg tagacaacca ggcgcgcgtc tgacacggac caacggtagc
     1801 aagtcttgct ccaacatttg gtcgcggtgc gatgaccaac cactgggtgg atatcaaaaa
     1861 cgctaacgtc gtaatggtaa tgggcggtaa cgctgctgaa gcgcatcccg tcggtttccg
     1921 ctgggcgatg gaagcgaaaa acaacaacga tgcaaccttg atcgctgtcg atcctcgttt
     1981 tacgcgtacc gcttctgtgg cggatattta cgcacctatt cgttccggta cggacattac
     2041 gttcctgtct ggcgttttgc gctacctgat cgaaaacaac aaaatcaacg ccgaatacgt
     2101 taaacattac accaacgcca gcctgctggt gcgtgatgat tttgctttcg aagacggcct
     2161 gttcagcggc tacgacgctg aaaaacgcca gtatgataag tcgtcctgga actatcagtt
     2221 cgatgaaaac ggctatgcaa aacgcgatga aacactgact catccgcgct gtgtgtggaa
     2281 cctgctgaaa gcgcacgttt cccgctacac gccggacgtc gttgaaaaca tctgcggtac
     2341 gccaaaagcc gacttcctga aagtgtgtga agtgctggcc tccaccagcg caccggatcg
     2401 cacaaccacc ttcctgtacg cgctgggctg gacgcagcac accgtgggtg cgcagaacat
     2461 ccgtactatg gcgatgatcc agttgctgct cggtaacatg ggtatggccg gtggcggcgt
     2521 gaacgcattg cgtggtcact ccaacattca ggggctgact gacttaggtc tgctctctac
     2581 cagcctgcca ggttatctga cgcttccgtc agaaaaacag gttgatttgc agtcgtatct
     2641 ggaagcgaac acgccgaaag cgacgctggc tggtcaggtg aactactgga gcaactatcc
     2701 gaagttcttc gttagcctga tgaaatcttt ctatggcgat gccgcgcaga aagagaacaa
     2761 ctggggctat gactggctgc cgaagtggga ccagacctac gacgtcatca agtatttcaa
     2821 catgatggac gaaggcaaag tcaccggtta tttctgccag ggctttaacc cggttgcgtc
     2881 cttcccggac aaaaacaaag tggtgagctg cctgagcaag ctgaagtaca tggtggttat
     2941 cgatccgctg gtgactgaaa cctctacctt ctggcagaac cacggcgagt cgaacgatgt
     3001 cgatccggcg tctattcaga ctgaagtatt ccgtctgcct tcgacctgct ttgccgaaga
     3061 agatggttct atcgctaact ccggtcgctg gttgcagtgg cactggaaag gtcaggacgc
     3121 gccgggcgaa gcgcgtaacg acggcgaaat tctggcgggt atctaccatc atctgcgcga
     3181 gctgtaccag gccgaaggtg gtaaaggcgt agaaccgctg atgaagatga gctggaacta
     3241 caagcagccg cacgaaccgc aatctgacga agtggctaaa gagaacaacg gctacgcgct
     3301 ggaagatctc tatgacgcta atggcgtgct tattgcgaag aaaggtcagt tgctgagtag
     3361 ctttgcgcat ctgcgtgatg acggtacaac cgcatcttct tgctggatct acaccggtag
     3421 ctggacagag cagggcaacc agatggctaa ccgcgataac tccgacccgt ccggtctggg
     3481 gaatacgctg ggatgggcct gggcgtggcc gctcaaccgt cgcgtgctgt acaaccgtgc
     3541 ttcggcggat atcaacggta aaccgtggga tccgaaacgg atgctgatcc agtggaacgg
     3601 cagcaagtgg acgggtaacg atattcctga cttcggcaat gccgcaccgg gtacgccaac
     3661 cgggccgttt atcatgcagc cggaagggat gggacgcctg tttgccatca acaaaatggc
     3721 ggaaggtccg ttcccggaac actacgagcc gattgaaacg ccgctgggca ctaacccgct
     3781 gcatccgaac gtggtgtcta acccggttgt tcgtctgtat gaacaagacg cgctgcggat
     3841 gggtaaaaaa gagcagttcc cgtatgtggg tacgacctat cgtctgaccg agcacttcca
     3901 cacctggacc aagcacgcat tgctcaacgc aattgctcag ccggaacagt ttgtggaaat
     3961 cagcgaaacg ctggcggcgg cgaaaggcat taataatggc gagcgtgtca ctgtctccag
     4021 caagcgtggc tttatccgcg cggtggctgt ggtaacgcgt cgtctgaaac cgctgaatgt
     4081 aaatggtcag caggttgaaa cggtgggtat tccaatccac tggggctttg agggtgtcgc
     4141 gcgtaaaggt tatatcgcta acactctgac gccgaatgtc ggtgatgcaa actcgcaaac
     4201 gccggaatat aaagcgttct tagtcaacat cgagaaggcg taagggggcg aacagatggc
     4261 tatggaaacg caggacatta tcaaaaggtc cgcaactaac tccatcacgc cgccttctca
     4321 ggtgcgtgat tacaaagcag aagtcgcaaa acttatcgac gtttccacct gtatcggctg
     4381 taaagcctgt caggtggcgt gttcggagtg gaacgacatc cgtgatgaag tggggcactg
     4441 cgtcggggtt tacgataacc ccgccgatct gagcgccaag tcctggacgg tgatgcgctt
     4501 tagcgaaacc gaacagaacg gcaagctgga gtggctgatc cgtaaagacg gctgtatgca
     4561 ctgtgaagat cccggctgcc tgaaggcgtg cccgtctgct ggtgcaatca ttcagtacgc
     4621 taacgggatt gtcgatttcc agtcggaaaa ctgcatcggc tgtggttact gcattgccgg
     4681 gtgtccgttt aatattccgc gcctcaacaa agaggataac cgggtatata aatgcacgct
     4741 ctgcgtcgat cgcgtcagcg tcggccagga accggcttgt gtgaaaacct gtccgaccgg
     4801 ggctatccac ttcggcacca agaaggagat gctggagctg gcggaacagc gcgtggcgaa
     4861 actgaaagcg cgtggttacg aacatgctgg cgtctacaac ccggaagggg tcggtggtac
     4921 gcacgttatg tacgtgctgc atcacgccga tcagccggag ctgtatcacg gtctgccgaa
     4981 agatccgaag atcgacacct cggtaagcct gtggaaaggc gcgttgaaac cgctggcagc
     5041 ggctggcttt attgccactt ttgccgggtt gattttccac tacatcggta ttggcccgaa
     5101 taaggaagtg gacgatgacg aggaggatca tcatgagtaa gtcgaaaatg attgtgcgca
     5161 ccaaatttat tgatcgcgtc tgtcactgga ccgtggtgat ttgcttcttc ctggtggcgc
     5221 tgtccgggat ttcgttcttc ttcccgacgc tgcaatggct gacgcaaacc ttcggtacgc
     5281 cgcagatggg acgcattttg cacccgttct tcggcattgc gattttcgtc gcgctgatgt
     5341 ttatgtttgt gcgttttgtg catcacaaca tcccggataa gaaagatatt ccgtggctat
     5401 tgaacattgt cgaagtatta aaaggcaatg agcacaaagt ggcggatgtt ggtaagtaca
     5461 acgccgggca aaagatgatg ttctggtcga tcatgagcat gattttcgtg ctgttggtga
     5521 ccggggtgat tatctggcgt ccgtactttg cgcagtactt cccgatgcag gttgttcgct
     5581 acagcctgct gatccacgcg gctgccggta tcatcctgat ccacgccatc ctgatccata
     5641 tgtatatggc attctgggtg aaaggatcga ttaaagggat gatcgaaggg aaggtaagcc
     5701 gtcgctgggc gaagaaacac catccgcgct ggtatcgtga aatcgagaag gcagaagcga
     5761 aaaaagagag tgaagaaggg atataatctc ttttgaactt taagctgaaa atggcgcttt
     5821 aagaagcgcc atttttttat tgcgtaacca gacggcgcag tcgcgacaca tccaccgttt
     5881 tttcagcctc tgccagactc caggcgtttt gcagattcag ccacatttgc ggcgaactgc
     5941 cgatcaccac ggaaagttta attgccattt ctggcgtcaa agctgctttt ccggtcagca
     6001 atcggcttgc cgttgagggc gcaatttcca ttgctctggc aaactcgcgc aggctgacat
     6061 taagttcgtc cagtgattcc tgaataatgt cccccgggcg gggatgattg gccattttca
     6121 ttagtgtctt tactctcagt agcgttcctt ctctgggaac actatcatta ttgttccttc
     6181 ctacgcaaca ataatccatt tagccatttc atgttggaga gtgtgcgatg ctgcgacccg
     6241 aacatgggag tcgcagcaaa gacctcttag tgacggaaat caatcaccat acggccacgg
     6301 attttgcctt cttccatctc ggtaaagatg gtgttgatgt ccgctaacgg acgcagggcg
     6361 actttcggca ccactttgcc ttcggcggca aactggaagg cttcggttag atcctggcgc
     6421 gtgccgacca gcgaaccgac cacttcaatg ccatccagca caagacgtgg gatatccagg
     6481 ctcatagact ccggcggcag accgacagcc acaacacgac cgcctgcacg gacagcatca
     6541 actgccgagt taaacgcagc tttagctact gctgttacca ccgcagcgtg agcgccaccg
     6601 gttttctcct gcacaattta ggcggcgtct tcggtgcgtg agttaatcgc taaatctgca
     6661 cccatttcgg ttgccagttt taactgctca tcattgacat caatggcgat cactttggcg
     6721 ttaaagacat tcttcgcgta ttgcagggcg aggttaccca gaccgccaag accgtagata
     6781 gcaatccact gaccgggacg aatttttgac agtttaacgg ctttgtaggt ggtgaccccc
     6841 gcacaggtaa tgctgctggc cgccgccgag tccagaccat ctggcacttt taccgcgtaa
     6901 tcggcgacca cgatgcactc ttccgccatc ccgccatcaa cgctgtatcc ggcattttta
     6961 actgaacggc agagcgtttc gttaccactg ttacagtatt cgcaatgacc gcatccttcg
     7021 taaaaccacg ccacgctggc acgatcgcct ggttttaatg aggtgacacc tgggccaact
     7081 tctgccacca caccgatgcc ttcatggccc agaattaccc cagttttgtc accaaaatcg
     7141 ccattcttaa catgaagatc ggtatgacat acaccacaac actccatttt cagcagggct
     7201 tcgccatgtt tcagtgagcg cagtgtttta tccgtaacgt caacatgatg atccttcgta
     7261 acaactgcag ccttcatagt tcctcctttt cggatgatgt tctgcatagc aggtgaggca
     7321 aatgagattt attcgccact acccagtatg gatgagatct gaaaaaggga gagggaaata
     7381 gcccggtagc cttcactacc gggcgcaggc ttagatggag gtacggcggt agtcgcggta
     7441 ttcggcttgc cagaaattat cgtcaatggc ttgttgcaaa gcttcagcag acgttttcac
     7501 cgccacgcct tgctgctgcg ccattttgcc aaccgcaaac gcaattgcgc gggagacttt
     7561 ctgaatatct ttcagttccg gtagtaccag accttcgccg ttcagcacca gcggcgaata
     7621 ctgagcaagc gtttcacttg ctgacatcag catctcatcg gtgatacgtg acgcgccgga
     7681 agcaataaca cccagcccga tgccagggaa aataaaggcg ttgttacact gggcgatagg
     7741 gtagatttta tctttccata ccactggatt aaacgggctg ccagtggcga ccagcgcgtt
     7801 accttcggtc caggcgataa tgtcctgcgg tgtggcttcc acgcgtgacg tcgggttaga
     7861 cagcggcatc acgatcggac gcggacagtg tttatgcatc tcacggatga tctcttccgt
     7921 aaacagcccg gtctgtcctg agacgccaat cagaatatct ggttttacat tgcgcaccac
     7981 atccagcagt gacagcacat cgctgtcggt atcccagtca ctgaggtttt cgcgtttctg
     8041 caccagtttg gtctggaaag gcagcaggtt cggcatcttg tccgtcagca ggccaaagcg
     8101 atcgaccata aagactttct gccgcgccgc ttcctcgctt aatccttcgc gctgggtctg
     8161 ggcgatgatc atttcggcaa tgccgcatcc cgctgaacct gcaccaagga agacgatttt
     8221 tttctcgctt aactgaccac ctgccgctcg gcttgctgcg atcagtgtgc cgactgttac
     8281 cgccgcagtg ccctgaatgt catcgttaaa agaacaaatt tcattgcgat agcggttaag
     8341 taacggcatc gcattttttt gagcaaagtc ttcaaactgc aacagcacgt ctggccagcg
     8401 ttgtttcaca gcctggataa attcatcaac gaattcatag tattcgtcgt caatgatacg
     8461 cggattacgc cagcccatat acagcggatc gttaagcagc tgttggttgt tcgttccgac
     8521 atccagcacc accggaaggg tatacgccgg gctgatgccg ccacaggcgg tatagagcga
     8581 cagtttaccg atcggaatgc ccatcccgcc gatgccctgg tcaccaagcc ccagaatacg
     8641 ttcaccgtca gtcaccacaa tcactttgat attatggttc ggtacgtttt gcagaatatc
     8701 gtccatattg tggcggttct ggtaagagat aaacacgccg cgtgaacggc ggtagatctc
     8761 agaaaaacgc tcacaggctg cgccgacggt tggggtataa ataacaggca tcatctcatc
     8821 aagatgattg tttaccagac ggtagaagag ggtttcgttg gtgtcctgga tgttacgcag
     8881 gtagatgtgt ttgtcgattt cggttttgaa tccctgatac tggatccatg ctcgttccgc
     8941 ttgttcttcg atggtttcga ccacttccgg cagtaacccc agcaggttga agttacggcg
     9001 ttcttccatg ctgaaggcac tgcctttatt caacaacgga aattccagca gtacagggcc
     9061 agcgtaaggg atataaagcg aacgctgttt ttttgttttt ggttccatgt cactcactct
     9121 tttttgaata tccatccctg gcacgctttt atcgtctttg ctttaccgcc agggcgtcgg
     9181 ccccaagtat aaagcagata aaaacaaaaa caccattgcg caggcaatgg tgtttaatcg
     9241 tcattgagga ctgatggtta tgaattactt ttcagcgggg cgttttctgc cggttgggtt
     9301 atttaccacg ctggatttgt caccttcggt aactattttg cgctggttag aaattttatg
     9361 gtccagtcca agaatatgac gtgcctgacg gttcgatttc attttaactc ctcaatcctg
     9421 ttgctagttt taaggacaac atcgccgtag cgaagaaaca cgtgctaaac ccctaaatta
     9481 ggttgccgat caagcatagc accttaaagc gtagggtgct ggccactgac cacataattg
     9541 atcgtttgct ggtagatatc actgaggatg tcgttatcag aagcttccac ccatttggtc
     9601 agctccatga gaatgtcatc ttcagtgaca acaccgtgct ctgcccgaag gccttgctca
     9661 atggcattaa ccagagcggg ttctgctgtt gaattttctg cctgataata agtaaacata
     9721 gtgattctcc gtgtctgtgt atttatggtg tttgctacgg atcgcagatt tataaagcac
     9781 attcagcatg gcaaatattt gccgctttgt tgttaagatt agtccttgtt gatgattttt
     9841 atattttaac ctcatgatat tgataggtat tattattgtg gtgagttgat taatattaat
     9901 gcaatatctc ttgggttata tattcccggt tatctattgt ggaaatttaa tttaagtgca
     9961 gaagtaatat ttctgccgga tttttattcg gaatatcctg cttatcctcg tgctgtttct
    10021 cacgtagtct ataatttcct ttttaagccc acatgagagc aacaatgaca atccataaga
    10081 aaggtcaggc acactgggaa ggcgatatca aacgcgggaa gggaacagta tccaccgaga
    10141 gtggcgtgct gaaccaacag ccgtatggat ttaacacgcg ttttgaaggc gaaaaaggaa
    10201 ctaaccctga agaactgatt ggcgcagcgc atgccgcatg tttctcaatg gcgctttcat
    10261 tcatgctggg ggaagcggga tttacgccaa catcgattga taccatcgcc gatgtgtcgc
    10321 tggataaagt ggatgccggt tttgcgatta cgaaaatcgc actgaagagt gaagttgcgg
    10381 tgccgggtat tgatgcctct acctttgacg gcatcatcca gaaagcaaaa gcaggatgcc
    10441 cggtctctca ggtactgaaa gcggaaatta cgctggatta ccagttgaaa tcgtaaagca
    10501 ttgccggatg acgtgtcagg cgcgtgaatg cctgatgcgt tgttagcatc tctacagcgc
    10561 ccgccagcac cggagctcac gtccgtcctc ccttattgct aatgattgcc ggacttcaca
    10621 tccgctggtt gcaagggggc aacgttcgta gaaaaaacag ccttgcggca acgtgcggtt
    10681 tcccggcaga tccgttttac gtaatgccca ttcttcctcc agcggtttat caatggcggg
    10741 gagggaatcc agcaataatc gggtgtatgg atgtgccgga gccgttagca cctgttgcgc
    10801 gtcgcccagt tctacaatct gcccgagata catcaccgcc acccgatcgc tcatatgacg
    10861 tatcaccgag acattgtgtg aaatcagcac ataggtcagc ccgtgatttt cctgtagcgt
    10921 taccagtaaa ttgaggatct gcgcctgcac ggagatatcc agcgcagagg ttggctcatc
    10981 aagcacaatc acgtcaggct gcgaagagag tgctctggca atggcgatac gttgccgctg
    11041 cccgccggag aacgcatgag gcaggcggtc gagatactcc ggacgaatac ccacctgcac
    11101 agccagctcc tctaccaacg ctcgccgctg ttgttcacta ctacgcttag ctatccagag
    11161 cggttctgtg atgatgcgcc acaccggtaa gcgcggattg agcgaagaga gcgggtcctg
    11221 aaacaccatc tgcataatgc gttgtgagcc agaacggatg tactgcccgt ggctcggttg
    11281 cagcataccc attaaaagct gtgcgagggt gcttttgccg cagcctgact cgccgacgat
    11341 ccctaaggtt tcaccacggc ggatctgtaa atcaatacca ttaatcgcat gaacatgttc
    11401 cgtagtttta ccaagccagt ttttacgggc cgggaaattg atatggacgc cgcgtaacgt
    11461 taataacgtg tcagacactt atgacctcct gctgcggata ccaacaggcg cagcgctggt
    11521 tgttgtcacc acacgctgtc agcgccggga cgttttcaca ctgtgcacct gccgcatagc
    11581 aacgatcgcg aaaagcgcag ccgtcaggca aatgggtgag gtttggtacc gtccctggaa
    11641 tggcgggtaa tagctggcgt ggtactccat gttctggtgc acattggagc aaaccaatgg
    11701 tatacggatg ccggggatga tggataacgt ctgccctcac gccgctttca attacgcttc
    11761 cggcatacat cacgtaaacg ctatcgcaca gttgcgacac cacggccata tcatggctga
    11821 tgaacagaac cgcagttcca ctggcgcggg ctttatgttt aagcaaacgc agtacctgca
    11881 actgcaccgt gacgtccagc gccgtagtcg gttcgtcggc aataatcaat tgcggctcgc
    11941 aggagaatgc cagcgcaatc attacccgct ggcgcatacc acctgaaagc tcaaacggat
    12001 agcgcgacat aacttccacg gcatccggga tttgcatctc ttccagcagg acaatcgctt
    12061 tagctctggc ttcccgacga cttattggtt gatgatggcg gatcacgtcc atcatctgaa
    12121 gacctattcg acgtgtcgga ttgagggcgg tcatcggttc ctgaaagatc atcgccactc
    12181 gtgcgccgcg ccactgacga agctgttttt cccgagcgtt aagaacatcg tctcccagca
    12241 gtgaaatctg cccccgatgt acgcaataac tgcccgtcgg tagcagacgc ataatcagca
    12301 ttgcggtgac tgatttacct gagccggatt ctcccaccag accgacaatt tcaccgcggt
    12361 taatctgcaa ggacacattg ttgagcgcgt gaacatcacc gttaaaaccg gggaaactca
    12421 aatgcagctg ttgaatgtcc agaacgggct gggtcatgac tgctttcctc ctgctttcgg
    12481 gtcaagcaga tcgcgaatac catcaccaaa gagattaaac ccaacggcgg taagcaaaat
    12541 cgctgcccct ggaaatgcgc aataccacca ctgatcgagt acatagttgc gaccattcgc
    12601 taccatcgcg ccccattcag cactcggttg ttgagcaccc aggccaataa atcccaacgt
    12661 ggcggccatt aaaatcgcgc taccgatatc cagcgatgcc tgcacgatca gcggcggcag
    12721 ggagttacgt aaaatatgcc agctgatcag atgccaacgc gacgcaccaa aggttttcgc
    12781 cgcctgaaca taggtatact ggcgtacaac taatgcttgc ccccgcgcca ggcgcacata
    12841 aaagggaatt cgcacaatag caatcgccag catggcgtta aataaactcg gcccgagagc
    12901 ggcagccagt gccattgtca gtaccaacga aggaatcgac agcataatgt ccatgatgcg
    12961 catgatgatg gcgtctgcgc gtccgccaag cacaccggat agacatccga gtagcgaacc
    13021 aatcataccc gcaatggcaa ccacgactaa tccggcgaga attgattgct gactgccgac
    13081 cagtacgcgg ctgaacagat cgcgtcccac ttcatcggtg ccaaaccagt gcgccgcaga
    13141 aggcggcaaa aggcgggcgg ttaaatcaat ggcgttggga tcatgcgtcg caagccacgg
    13201 tgaaaaaaac atcatcaata gcattaatac aatgatgact gcgccggtca cggtgagcgg
    13261 actgcctttc agcatccata tcagttttgc accgttaaat cgcgtttgtt tttgtgggcg
    13321 tacggcggac gtttcctcgc ttagcatcat tcaccacctc cacgtccgat acgcggatca
    13381 atccacaaat agagcaaatc caccaccagg ttgaccagca cataagcaaa tgaaaccacg
    13441 acggcaaagc ccatcactgc cgggaagtcg agcgcctgta ttgatgttac tacccatgca
    13501 cccattccgg gccaggcaaa aacggtttcg gtgagcactg cgccatacaa caaatcgccc
    13561 agcgccagac ccaatacggt aatcgatggg atcaacgcat tgggtagcgc ataacataaa
    13621 acgatatacc agccgggcaa gccgctggcc cgggcggtac gaatgtagtc ttcactcaat
    13681 tgttccagca tcgctgagcg gatctggcga gcgacaattc ccaggtgaac gaacgccagc
    13741 gttaatgccg gtaagatgag atgttgcaac gcattgaaga agacttcacc gttgccttca
    13801 agcagcgcat cgagcagata aaagccggta acgtgcgttg gtggatccag ccagtcatca
    13861 agccttccgc cgccgggaag aatttgcaga tgaccataaa acagcacaat gacgcccagt
    13921 ccaagccaaa acgcaggtgt ggagattccg gtaatggcca ttattcgcac cagatgatcc
    13981 agccagcgat ttcgccagac tgcagagagt atgcccagcg ggatgccaat caggagtgcc
    14041 agcagcaggg cacaaaaagc cagttccagc gtcgccggga aaaatatacg caactcttcc
    14101 aacaccggac gcccggtacg aatggatgtt cccaggtcac catgaaacag gtcgctgacg
    14161 tagcggtaaa actgtacata cagtggctgg tccagtccca gttgctggcg aatattttcc
    14221 acgatagcat cgctggcgcg gtcacccgcc agtaaccgcg ccggatcgcc agggatcaga
    14281 tgcgagataa taaacgtaat cacgcagacg cccgccatca cgagcaccag cccccagcag
    14341 cgttggcgta aaatactcca gaaggtcacg ttatttactc atggtattga tattgaagac
    14401 ctgttccagc atgggattga acacaaagcc tttcacctct ttgttcatcg ccagttggta
    14461 gtttttctgg aacaggtaca cataagcagc gtcatcaatg acgattttct gtgcctgctg
    14521 gtagtcccgg gtacgctgcg tctggtcggt ggtcgcaagc gcattgcgca gtaacttatc
    14581 gacctcactg ttttcataga acgagcggtt acccggcaga ccttttttgt ctgactcaaa
    14641 ccagtaattc ataaacatat acgggtcggc aaaatccgga ctccagttgc caatcgcaat
    14701 gtcgtaatca cctttaccca ctctgtcgcg catggtggcg ttcgccagct tttccagctt
    14761 cacattgatg cccagcttgt tgagactgga ttgtgtcgcc agagcaatag gctcccagtt
    14821 cggatcctta tcggagtaga gaaacgtcag gctggtgggt ttgctcgtca ctttatccca
    14881 ttcggctttg gctttcgttt cgtcatggtt gtattgcatt gccgtcgcat cgtagcccca
    14941 catgccttcc ggaatcgggc cgcgcatctg ttttccgtta ccactcagaa tgccgttaac
    15001 catgccctga taatcggtag accaggaaat ggcccgacgc agatccgcct gattaagagg
    15061 cgctttgctg ttattcagat acaggtaggt gacgcgcagt gacggatact ctgccacatt
    15121 gactttgttt tcctgcttca gggcgttgag ttgatccacc ggcagcgcat cggcaatgtc
    15181 aatatcacca cgggagagct gcaggcgacg ggaggcactt tcaccaataa tttttaccga
    15241 tactcgctta aagttcggtt tattgccagg gtaatgcgga tttggcacca gaactaattg
    15301 ctgacctttt tgccagcttt tcagcataaa tggtccggaa ccggcggtat tttgcgcgag
    15361 gaagccgcgg gcatcatccg ctgcatgttc ctttaacacc gccggattga taatggatgc
    15421 accgtcattc gccagcgtgt agaggaacgg tgcgaatggc tggctaaggg taaacttcac
    15481 cgtatgttcg tcgggagcat caatctttaa atctttggga aatgcttctg ctggcccctg
    15541 gccgattttt agtagccgct caaaagagag ttttactgct tcggcagtga ccggtgtgcc
    15601 atcggcaaat ttagcattgt ccttcagggt gaacgtccac tctttttgat cgtcagacgc
    15661 tttccagcta cttgccagat cgccttcaac gtcggttgag cctttatcac cgtccgtttt
    15721 gtactgaacc agccgctgat aagacgggta ggtcaccgtc cagtcgttat tatctattgt
    15781 taccgccggg gcgagggttt gtggatcggc ggccttacca atcaccagca tatctttcgg
    15841 tacggcggcg tgcgcaaccg ggaaagttgt ggcaaggacg agcgcgagca atgtaggacg
    15901 aaacggtatc gatctcttca tgacagtttc ctgattaact gacgtgctgt gtgccagggg
    15961 atataaaaca ggtgaattga tcagcgagca gtgggtaact cgctgcctgg ggtaattcga
    16021 agtgccacca ttcgctggag ataccgacaa aaccgccacc agtcataatc gcattcagca
    16081 acagccgatt gcgctgagcg gcgggcggga cggaagggtg ataggcatgg gagcgctcgt
    16141 gcatttcatc gaaccctgcg cccatatcga ggatgttccc gtgctcatca cgaagcgtca
    16201 ggtcgatcgc cgtgccacgg ctgtgattag aaccgaccgt cacatcaaca acatattgcg
    16261 ggtctgggca ggcttgccac aacatcgcct gtgcttgttg tgggcgatac gcatcgtaaa
    16321 tcaccagttg taagcctgac agctgggcga tactgatact tttcgccagc gcggtaatcg
    16381 catccttgtg tagcagacaa cgcgcttgct gataaatagc tttacctgtg atgttatcag
    16441 cgcaggcgta tttcaattcg atctccagat cagggaagat tacggctaaa tcaaccagtt
    16501 cggtggtatc cgacataaac ggttcctgta taagacaaaa attgctgcgc tttccgctta
    16561 tgcagatctc atgccatgcc gggataagcg ccagaatgct ggcttaaagt tatatttttt
    16621 aacttttgat caacatttgt gcagcgtagt gcagttttgg tgcaagaggg gaagttaagg
    16681 aaggaatctc ccggaatcgt agatgaaatc acagtattta agtgacggtg tcacgttaaa
    16741 tgaaaacccg cgagtgcggg cgagaggaat ttgtcagatt ttcagcggta acacgctgct
    16801 catccagcct ggaatttctt cggcgggtag ggggcgggaa aagaaatatc cctgaataac
    16861 gcgacagtgg atcttgcgta gcatctcaaa ttgctctttg gtttcgacgc cttccgccac
    16921 gacggttaaa ttgaggcttt gcccaatgct ggtaatggct tcaagtaagg caaggatgcg
    16981 tttttcggtc agacaacgat cgacaaaact tttgtcaatt ttgatttccg ttaccggaag
    17041 actgactaag cgggataatc cggaaaagcc cgtaccaaaa tcatctaccg ataagcccac
    17101 gcccatatca cgcaggatct gaatgcgctt aaagatttcg gtatcgtgtt ccatcatcat
    17161 gctttccgtg atttctaccg tcagctggtg gccgtcaata ccccaggcgt gcattgcatc
    17221 agacacctga ttaggcagtt gattactgcg aaagtgcagt gccgacaagt taacggaaag
    17281 cgccgggata tgaatattct ggctacgcca ttctgctaac tgacggcaag cttccgcgat
    17341 gacccagcgc ccaatatttt cgatttcacc aatctcttct gcgagaggaa taaaccgtga
    17401 agggggcaca tgaccatgct ggggatcgtg ccagcgcgca agggcttcga tgccgtacag
    17461 ttcacccgtt tctgcgaaga tttgcggctg gtaaacaagt ttcagttggt tattgctaat
    17521 cgcttctttc agcgctgcgc ctaaaaccaa acgctctttt accatttcgt tcatcgccgg
    17581 gctgaagaac tgccagccgt taccgccatt cttgcgaata taatctattg cattgtgagc
    17641 agtggagagt aggtaatcgt ggtttttacc cacgtcgtag ctgatgccaa tactcaaggt
    17701 aagcgggaat ggtttatcgt caatcattat cggcttgctg accacattcc gtagctcatc
    17761 ggcgatctgg gtaatgttac tgacgtcgtt ttccaggctc acgaggacaa actgcgtacc
    17821 ttcgatacga cagagatact gatccggttt gagttttcca cgaaagcgat tgaccacttc
    17881 cagcaatgcc tgatcggccc acgcatagcc aaggctatca atcacatcct gaatatggtc
    17941 aacaccgatg agatacacca ccggagagac ggctttgtcg accaggtcat cgaggtaatt
    18001 gtgcaggtta ttgcgatttg gcagaccggt catcggatca aattggatga gttgttcaat
    18061 atgctggcgg cttttttcct gttccagcgc cagcgcggcc atatgctgac tgatatctgc
    18121 cacgcgttcg ataaaggcgc tggtttctgc tcctgacgag gttttaattt gcaggatccc
    18181 cgcaggcgcg ccatcacgct gacgaatggt cgctgaccag ctttgcgcat tttgaacttc
    18241 tgcaccgtgc gaagatgacg cccagtgtat cggcatcccg ttgcgcagtg cgaacagcga
    18301 aacatgcgat tcgttgagaa cagattcgat gttacgacaa atgatttccc ccatttcatg
    18361 aaatggcggg ctgctgcaca tggcggcgag aatattgcct tcaagctggc ggatttgccg
    18421 ttcttcggtg atatccgaga aagtcattac caggttctgc agatgcgcga gcacgtcata
    18481 aaccgggctg atagaggctt taatccagat tttttcaccg gtgcgcgtca acagcagaaa
    18541 ttcgtcctga tcgcgggcgg ttttccatag caactgttgt aaacgaatgc ggttatcggc
    18601 agggaattca ggaatgttca ggagtgtatc gggctgcata ccgctggctt cgctaatgca
    18661 gtaaccaaac atttcggtaa atgcgcgatt gcactgcaca atatggcgtt ccggatcgag
    18721 gacaatcacc ggtcggtcga gatggtcaac ggcaataatc aattgtcggg tctgttcttt
    18781 ttgcgccatt tctacgctgg catcccgtac cagcgccagg taataaactt tcccctcggc
    18841 gctcactttc gatagcgcaa aacgggtcca gattttactg ccgtcttttt tctccagctg
    18901 cagctcccga ctcatcccct cgacgcgcgc tttaccacct tcacggttat gacgaatgta
    18961 ttcaggatgc gcaggacgca aatcccgcgg aatcagcata tcaatgttat tgccaatgac
    19021 ttcttcacgt ttgtaccccc agagcttctc tgcggcgggg ttgaaaaaca tcacttcatc
    19081 attttcgtta attaacaccg cgcccatcat attttgttca agggcgggga aaaaaatgcc
    19141 atcggcggca ttttccgcat cggttagctt catgattacc tctgcatcct ggcgcatcta
    19201 aagactggct ttccagagtt caacacggtt tctacctcgt cttttggcga tatacagagc
    19261 ttcatcggct atttgaatga ggcgctcata gtcaggatga ccattaaaca tggcggcacc
    19321 gatggaaagt gagagggcaa tatcctcgcc gtttgcggct ttcagtttgg ttttctccac
    19381 ccgactgcga atacgttctg cggtacgtaa cgtttcgttt tcagaagctt cagtcaaaac
    19441 aatgataaat tcatcgcccc cgtagcgaaa aacataatca ctactgcgga cgttgtcata
    19501 aaaggcctga gagactttac gcagaatttc atcgccagtg ttatggcccc acgtatcgtt
    19561 gatctctttg aatttatcaa cgtcaataat cagcactgac agcggtgtac cggtccggtt
    19621 ggcatgggca atttcgcgtt tgaagatagt cggcaggaag cggcggttaa gtaatttcgt
    19681 cagtacatcc ataccgactt cgtggcgcga tacttcttca aacaattcac gcagcaaggt
    19741 aataatttgc gatacagtat ttcttatctg taataaaaat ttcacccgca gacttctgtt
    19801 attcaaattc ctggtgttac gcatggtttg attgaatata ccgtcgaaat cctgaatcag
    19861 acgggagata tgcctacttc ggcaatgcca ctaaaataat gtcgaccttt atggttaaac
    19921 cacaggccaa aatcagcctg gcttaaaggc aaactactgc ctaaatcaga atccagcagg
    19981 attttataga taatatctat ttcccatgaa agtattgagg ctatttgccg ttctttttct
    20041 tcttcggcgt tttctagtaa cgagaagata cgatagtttt catcttcctt tgaggcacta
    20101 ctgtcactaa aggtaaacgc gcgggtcatc acttccatag cgatatcaat actgttaatc
    20161 gagaaatggt agacctgaag tttttctgcg gcagaataat ccgaagagaa gattaccgga
    20221 tagaggattt ttttcagcac ccgaaacccc atctcgacaa tttctaccgg aattcctatg
    20281 cgggcatgca cttccgcgac ggtatgctgg atttgtatta gcctttcgac atcgtcaacc
    20341 tgggcagaaa gcacgttaat aatccagcgt tccatcgcac ttttcaactg ccgctcaact
    20401 tgttcattac tcaagaattc ttcggcatgc gggtcgatgc ggacaattcg ataaaactcg
    20461 atactcagat aatgagcatg cgcaacggca atttccgcgg ctttagcacg aatgagtgga
    20521 tctgcctgtt cgacaagtcc ggtccactca tctttcattc ttttaaaata catctccata
    20581 attcacaccc ttataaggct gggaaatcag acggaatcaa aacgcaacgt gcgagatcga
    20641 ctaactgcac catattctcc tgaaatatga agatatactg aaaagaaata agcgacttag
    20701 gacagtttca atctacgcta ctgttcttca gaagagtata gcccatcgta attatttttc
    20761 ggtgacagcg aatatcgtat ggtttttcat attcatacat ttttattagg gatttatagc
    20821 tgtttaacta agtgtggtta attgcgtggt taatttgact tatgtaagca tgattactag
    20881 tgggatattt cacaaggtgt aacaagccgg tgggtaaagc accggcttgt tacaaagtaa
    20941 gcatgggagt ttaactgccc caacgacttt gcagatagct gaccgcttgt tgagtctgcg
    21001 gtttattcag atagtcctca cggaacaaga tggtgccgct aatttctggt accgcatcgt
    21061 taagatcgag ctgctttttc agttccggta cgccgccgtt aatcatccag tctggctcta
    21121 tctttgaagg ttcacccact ttatagaagg cgataccgat atacaggcgg gtcctggtcg
    21181 gtttaacgac atccgcccac cattttgcca acacgtcata acgcgcggca ctccgtgaga
    21241 acggccagta aatttgggga gcaatgtaat ccagcaatcc ttgttccacc catcgacggg
    21301 tgtcagcgta ggattcgtca taggctgccg cgcctcgggt atcggaaccg agcggatcgt
    21361 gtgatcggtt acgccacacg cctgccgggc taacgccaaa ttcgactccc ggcttaatgc
    21421 ttttaatggt gtgcgatact tttgtaatta actgctgagt attgttgcgc cgccagtctg
    21481 cttttgacgc aaatgcgcct ccgtatttac ggtacgtttc gttatcattt agccgtgaac
    21541 ccggtgactc cgtatagaaa tagtcgtcaa actgcacgcc atctaccgga tagcgggaaa
    21601 ccacttcggc gactattgat gtgatccagt cctgaacctc agggatgccc gggtcgagga
    21661 caaagcgatc gccagacgtt ctgatccagt cacggtgttg cacatagacg ctcgccggtt
    21721 gttgagacag agtgctattc agttccctga tagtaccggg cttcgtatta accgatacgc
    21781 gataggggtt aaaccaggcg tgtactttca tcccacgctt gtgggcttcg tcgagcataa
    21841 attgcagcgg atcgtaaccc ggattttcac caatcttacc ggtcataaga tcggaccacg
    21901 gcaaaatttt tgatggccac agggcggtac cgtccggctt gacctggaaa aagaccgtgt
    21961 ttataccgag acgttgcaga tgatccagtt tgtcgatcat cgcctgttgt tgtacacggg
    22021 cccggctggt ggggttacta atgttaaccg aggaaaccgg tggccagtcg aggcgagaca
    22081 ccgtggccag ccagatgcca cgcatcggtt gtgacgattg ttgcgtcgtg gctggtggct
    22141 ttgaacctgc tggtggtgtc accatggact ctggaggcgt gcttttacaa ctacacagta
    22201 aaagtgccag tgcaaccagt atcgctggtc ttctaatcgt taatttcttg tttcgggagc
    22261 agatatccat atgtgctggt ttccggtaaa cagatgtgcg ctcattctcg tacttatccc
    22321 ggttaagtca atacgacagc aagcacgaaa aagggagcga tgaattatcg ctcccttgtc
    22381 ttataaccat tcagacatgg ttagtgtttc ttgtcattca tcacaatata gtgtggtgaa
    22441 cgtgcacgcg ggtgcaggaa gaagtgacct tttggtgcgt tctgactgtt gattggctcc
    22501 agagtgacgc cggtattcgc tttgccttta cgatcatgaa cagcatagag aataaagggc
    22561 agggcaagta ccaccaggaa actaacgacc agtaattcaa catacatatc ggtagagtca
    22621 ccctggatgt tatccggcgg caggaaggaa acaataaacg ccataattga agtcagcaga
    22681 ccgacaattg ccacgaccag tttcacccct ttaccaccag ggatattaaa tgtgcgtttt
    22741 aagtcaggat gtttaagaac caacacaatg tagccaataa acagcatgaa ataagcacac
    22801 agataaatca ccaccgtcag cgccagtgcg atcaggaagg acatgttgtt accgccaccg
    22861 gtattggtga ggatgatcaa cgcgatagaa gtaatcacca gctgcgaaat gaccagcgtt
    22921 accggtacgc catttttgtt cattttagcg aatgccgctg gcagcaggtt tttctgcgct
    22981 gtgacataca tcccgcgaga aggaccaaca atccaggagg cgatttccgc cagaacaccc
    23041 agcaacagca gtgcggagat cacgcgaacc gtccactcaa tttccggtgc cacatgggac
    23101 atcagaacgg taaaggtttg cattacccct gcggagaggt tgatttcatt acccggaatg
    23161 accatcgcaa tagataaacc gccaacagag cttaagcaga ttgccgccac catcagcagt
    23221 aacatagcca gtggatagtc gcgcccgggg ttgctcattt cattgacgtg agttgcggaa
    23281 gcttctacgc ccatataact caaaatgaag gcaacaaata caaccagtgt gcccacttta
    23341 gagaagtcag ggaagaaggt cttcgaatcc atttcgatag caacgggggc accggagtgc
    23401 agataaatag ccgctaatgc gatcaaaata aatgcaggta acaggatacc ggcgaagaag
    23461 ccaactttag caattcgcgc cgtgtatttc gtgccaccaa actgcgttaa tgccagcgcc
    23521 caaagaatga tgagtgctgc aatagttttg gtaatggggt cttcattcag cgctggccat
    23581 ttcaggatgt aggagagtgc ccctaacacg aaatagagca tcggaataaa accaatggcg
    23641 atttgcagat agccaaatga gatcgctgca aatccccatc tcggccccag agtatttgat
    23701 acccaggcga agacaccacc ttcttcccag ccgtcgacgg tggccatttc cgcagcacaa
    23761 agtcccacgg gaataaacca taaaatcccg cctaatagca ggaagaagac taatgaaaag
    23821 cccgatgttg cgaaggtagg gtattcataa acagccatta ccatcgatgc cgttatggca
    23881 aagaatccga gtaatgtgag ctgcttagct ttacctgtct gtactgatgt agccatatta
    23941 tccccctaaa acggtattcc tgtcggaacc gcacctgttt tgttctgaag cgtattcaga
    24001 acaatatttt ccgttgctaa tgccagtgaa cagactttgg aaattgtccc gaaacgggtt
    24061 cgtttcggga caccgttacc gttaaacgtt atcaggtatg tttaaagctg ttctgctggg
    24121 caataccctg cagtttcggg tgatcgctga gatatttcag ggaggctttg tagtcttcca
    24181 gcaacagttc agcaaagtcc atttcgaagc cgcgacgaca cataat
//
LOCUS       DAZPEB010000041        24060 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZPEB010000041 DAZPEB010000000
VERSION     DAZPEB010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24060
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.042"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            322..549
                     /gene="feoA"
                     /locus_tag="ACHURJ_001483"
     CDS             322..549
                     /gene="feoA"
                     /locus_tag="ACHURJ_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFR1280486.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            566..2887
                     /gene="feoB"
                     /locus_tag="ACHURJ_001484"
     CDS             566..2887
                     /gene="feoB"
                     /locus_tag="ACHURJ_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFR1280487.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAIDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            2887..3123
                     /gene="feoC"
                     /locus_tag="ACHURJ_001485"
     CDS             2887..3123
                     /gene="feoC"
                     /locus_tag="ACHURJ_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFR1280488.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            3326..4204
                     /gene="rpnA"
                     /locus_tag="ACHURJ_001486"
     CDS             3326..4204
                     /gene="rpnA"
                     /locus_tag="ACHURJ_001486"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HFR1280489.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMGCLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLTDI
                     MRFTGVSEEELAAASQ"
     gene            complement(4233..5003)
                     /gene="bioH"
                     /locus_tag="ACHURJ_001487"
     CDS             complement(4233..5003)
                     /gene="bioH"
                     /locus_tag="ACHURJ_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFR1280490.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSQGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDDWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPRSKSYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            5041..5724
                     /gene="gntX"
                     /locus_tag="ACHURJ_001488"
     CDS             5041..5724
                     /gene="gntX"
                     /locus_tag="ACHURJ_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFR1280491.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCRWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            5783..6358
                     /gene="nfuA"
                     /locus_tag="ACHURJ_001489"
     CDS             5783..6358
                     /gene="nfuA"
                     /locus_tag="ACHURJ_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFR1280492.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            6719..8035
                     /gene="gntT"
                     /locus_tag="ACHURJ_001490"
     CDS             6719..8035
                     /gene="gntT"
                     /locus_tag="ACHURJ_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFR1280493.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(8080..10164)
                     /gene="malQ"
                     /locus_tag="ACHURJ_001491"
     CDS             complement(8080..10164)
                     /gene="malQ"
                     /locus_tag="ACHURJ_001491"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFR1280494.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLETMFADDGVNKLLKD
                     LDRRRRAAAKKQ"
     gene            complement(10174..12567)
                     /gene="malP"
                     /locus_tag="ACHURJ_001492"
     CDS             complement(10174..12567)
                     /gene="malP"
                     /locus_tag="ACHURJ_001492"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFR1280495.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQEVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATIGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKLLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLQDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            13179..15884
                     /gene="malT"
                     /locus_tag="ACHURJ_001493"
     CDS             13179..15884
                     /gene="malT"
                     /locus_tag="ACHURJ_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFR1280496.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIATVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(15927..16943)
                     /gene="rtcA"
                     /locus_tag="ACHURJ_001494"
     CDS             complement(15927..16943)
                     /gene="rtcA"
                     /locus_tag="ACHURJ_001494"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFR1280497.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGAVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIEIDGVTR
                     VSIE"
     gene            complement(16947..18173)
                     /gene="rtcB"
                     /locus_tag="ACHURJ_001495"
     CDS             complement(16947..18173)
                     /gene="rtcB"
                     /locus_tag="ACHURJ_001495"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFR1280498.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKDSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAEDLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIKICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDVMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMTAQSD
                     LVEVIYTLRQVVCVKG"
     gene            18362..19960
                     /gene="rtcR"
                     /locus_tag="ACHURJ_001496"
     CDS             18362..19960
                     /gene="rtcR"
                     /locus_tag="ACHURJ_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HFR1280499.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWHPTLCLCQQESLVIDRL
                     ELLHDARSCSLFETLKRDIASVSPETEVVSIEIELHNPWDFEEVYACLHDFARGYKFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQTSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFFDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSATGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(19942..20700)
                     /locus_tag="ACHURJ_001497"
     CDS             complement(19942..20700)
                     /locus_tag="ACHURJ_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFR1280500.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(20717..21547)
                     /gene="glpG"
                     /locus_tag="ACHURJ_001498"
     CDS             complement(20717..21547)
                     /gene="glpG"
                     /locus_tag="ACHURJ_001498"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFR1280501.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVVMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIIAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(21592..21918)
                     /gene="glpE"
                     /locus_tag="ACHURJ_001499"
     CDS             complement(21592..21918)
                     /gene="glpE"
                     /locus_tag="ACHURJ_001499"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFR1280502.1"
                     /translation="MDLFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            22108..23613
                     /gene="glpD"
                     /locus_tag="ACHURJ_001500"
     CDS             22108..23613
                     /gene="glpD"
                     /locus_tag="ACHURJ_001500"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFR1280503.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(23665..>24060)
                     /locus_tag="ACHURJ_001501"
     CDS             complement(23665..>24060)
                     /locus_tag="ACHURJ_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1280504.1"
                     /translation="SVMAGIGWQFSRRTPPELKNRLHCWLVFSPVYFWLILSNFILYI
                     SPEKSALLENIRNFFLTFVWPPLNFSPFWPQPWTDFVGPISAQLGFALGYYCHWCRKN
                     RNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
BASE COUNT         5655 a         6552 c         6080 g         5773 t
ORIGIN      
        1 caaactcata atatattccg gcaatattta tcacttcatt aacaactgaa accttaatta
       61 aacattagcc agtccgggta attcactatt cgaattatat tttcgctgcg atataacctt
      121 gagccacatc aacattgagt cagattatta ttcaaaccaa cattcgcaca cattttaagt
      181 attgctgata gaaaccattc tcattatcat tgtgttattg attatttaat ctctcctttg
      241 ttggcaaatc atctggtctc atgtcgctgt caaacgcccc atgaggtagt tatccagtta
      301 atgagaaaca agtaggcacc tatgcaatac actccagata ctgcgtggaa aatcactggc
      361 ttttcccgtg aaatcagccc ggcatatcgc caaaaactgc tttctcttgg catgttacct
      421 ggctcctctt ttaatgtggt gcgcgtcgct ccactcggcg accccattca tatcgaaacc
      481 cgtcgtgtga gcctggtatt acgcaaaaaa gatctggcct tattagaagt ggaagcggtt
      541 tcctgttaat acggtgataa caacaatgaa aaaattaacc ataggcttaa ttggtaatcc
      601 aaattctggc aagacaacct tatttaacca gctcactggc gcacgtcagc gtgtaggtaa
      661 ctgggctggc gttaccgtcg aacgtaaaga agggcaattc tccaccaccg atcatcaggt
      721 cacgctggtg gacctacccg gcacctattc tctgaccacc atctcatcgc agacctcgct
      781 cgatgagcaa atcgcctgtc actacatttt gagtggcgac gccgacctgc tgattaacgt
      841 ggtggatgcg tctaaccttg agcgtaacct gtacctgacg ctacaactgc tggaactcgg
      901 cattccctgc attgtggcac tgaacatgct cgacattgcc gagaagcaaa atattcgtat
      961 tgaaattgat gctctgtcgg cgcgtctggg ctgtccggtg atcccgctgg tttcaacccg
     1021 tggtcgcggt attgaagcgc tcaagctggc gattgatcgc tataaagcta acgagaatgt
     1081 ggaactggtg cattacgcac agccgctgct caacgaagca gattcactgg caaaagtgat
     1141 gccttccgac atcccgctga aacaacgtcg ctggctgggc ctgcaaatgc tggaaggcga
     1201 tatctacagc cgcgcctacg ccggtgaagc gtcgcagcat ctggatgccg ccctcgcccg
     1261 tctgcgtaat gagatggacg atccggcgct gcacattgcc gatgcgcgtt accagtgcat
     1321 tgctgccatc tgtgatgtgg taagcaacac cctgacggca gaacccagcc gtttcaccac
     1381 tgcggtagat aaaatcgtgc tcaaccgttt cctcggtctg ccgattttcc tctttgtgat
     1441 gtacctgatg ttcctgctgg ctatcaacat cggcggggcg ttacagccgc tgtttgacgt
     1501 cggctccgtg gcgctatttg tgcatggtat tcaatggatt ggctacacgc tccacttccc
     1561 ggactggctg actatcttcc tcgcccaggg cctgggtggc ggcattaaca ccgtgctgcc
     1621 actggtgccg cagattggca tgatgtacct gttcctctcc ttccttgagg actccgggta
     1681 tatggcgcgt gcggcgtttg tgatggaccg tctgatgcag gcgctgggct tgccggggaa
     1741 atcctttgtg ccgctgatcg tcggtttcgg ttgtaacgta ccgtcggtaa tgggtgcacg
     1801 tacgcttgat gcaccgcgtg aacgtctgat gaccatcatg atggcaccgt ttatgtcctg
     1861 cggcgcgcgt ctggctatct tcgcagtatt cgcggctgcc ttcttcgggc agaacggtgc
     1921 gctggcggtc ttctcgctgt atatgctggg tattgtgatg gcggtgctga ctggcctgat
     1981 gctcaagtac accatcatgc gcggtgaagc gacgccgttt gtcatggagt tgccggtcta
     2041 tcatgtacca cacgttaaaa gcctgattat ccagacctgg cagcgtctga aaggcttcgt
     2101 tctgcgtgct ggtaaagtga tcatcatcgt cagcattttc ctgagcgctt tcaacagctt
     2161 ctcgctgagc gggaaaatcg tcgataacat caacgactcg gcgctggcgt ccgtcagccg
     2221 ggtgatcacc ccggtcttca agccaattgg cgtgcatgaa gataactggc aggcaacggt
     2281 tggcctgttt acaggtgcca tggcgaaaga agtggtagtg ggtacgctca acaccctcta
     2341 caccgcagaa aatattcagg acgaagagtt caatccggca gagtttaacc tcggtgaaga
     2401 gctgttcagt gcgatagatg aaacctggca gagcctgaaa gacaccttca gccttagcgt
     2461 actgatgaac cccattgaag ccagcaaagg caacggcgaa atgggtaccg gggcgatggg
     2521 cgtgatggat cagaaattcg gtagcgcagc agcagcttac agctacctga ttttcgtcct
     2581 gctgtatgta ccatgtatct cggtgatggg agccatcgcc cgtgaatcaa gccgtggctg
     2641 gatgggcttc tccatcctgt gggggctgaa tatcgcttac tcgctggcaa cattgttcta
     2701 tcaggtcgcc agctacagtc agcacccaac ttacagcctg gtgtgcattc tggcggttat
     2761 cctgtttaac atcgtggtta tcggtctgct gcgccgcgcg cgtagccggg tggatatcga
     2821 actgctggca acccgcaagt cggtaagcag ttgctgcgca gccagcacca ccggtgattg
     2881 ccattaatgg cttcacttat tcaggtgcgc gatttgctgg cgttacgggg ccgtatggaa
     2941 gcggcccaga taagccagac attgaacact ccacagccaa tgattaacgc catgctgcaa
     3001 caactggaaa gtatgggcaa agccgtgcgg attcaggaag aacctgacgg ctgcctctct
     3061 ggcagttgta aaagctgccc ggaaggaaaa gcctgtctgc gcgagtggtg ggcgcttcgt
     3121 taaccttact ccatcgcctg ttttggaaag cccggtatgc gtctgcatcc gggctttttt
     3181 gcgtgcggct ttccataaaa atgcaactct tgcagcacgg cgtaagttcc tttgaaagca
     3241 tctcgcaggg atgaaaactc gctaatacac aggtgtggag tggcgcgtag agtcgcggca
     3301 ttcaaacaac aggtgaagga acgccatgag caaaaagcag agttccaccc cacacgatgc
     3361 gctgttcaaa ctctttttac gccaaccgga cacggctcgt gattttcttg cgtttcattt
     3421 accggcaccc attcacgcgc tttgtgatat gaaaaccctc aagctggagt cgagcagctt
     3481 tattgatgac gatctgcgtg aaagctattc cgatgtgctg tggtcggtga aaacggaaca
     3541 aggaccagga tacatctatt gtctgattga acatcaaagc acctcaaaca aactgatcgc
     3601 atttcgcatg atgcgttacg ctattgccgc aatgcaaaat caccttgatg ctggatacaa
     3661 aacgttgccg atggtggtgc cattgttgtt ttaccacggt attgaaagcc cctatcccta
     3721 ttcgctgtgt tggctggatt gtttcgccga tcccaaactg gcaaggcagc tttatgcctc
     3781 cgcatttccg ctgattgatg tcaccgtcat gcctgatgat gaaatcatgc agcaccgacg
     3841 tatggcgctg ctggagttaa ttcaaaaaca tattcgtcaa cgcgacctga tggggctagt
     3901 agagcaaatg ggctgcttat taagtagtgg atacgctaat gacagacaaa tcaaagggct
     3961 gtttaattac atactgcaaa ccggcgacgc ggtacgtttt aacgatttta tcgacggcgt
     4021 tgccgaacgt tcaccgaaac acaaggagag tttaatgaca attgcggaaa gattgcggca
     4081 ggagggggaa caatccaaag ccctgcatat agccaaaata atgcttgaat ccggagtccc
     4141 tcttacagac atcatgcgct ttaccggggt gtcagaagaa gagttggctg cggcgagcca
     4201 gtaaatttct gtctcgccat ttcaaaagtt gcctacaccc tctgcttcaa cgccaccagc
     4261 aggtgacaaa actcgaccgg atgcgaaata aacggcgcat gggccgcttt ggcgaagata
     4321 tatgatttgc tgcgaggcca gagtttatcc agcatcggca ccactttgcg cggcaccaga
     4381 ccatcgagat agccatacaa tcgcaaaaac ggcatgggca cgttttgcag cggctgacgg
     4441 agatcaaccg ttttcaggat ttccagcccg ccattaagca cgtcaacctc cggcatcggt
     4501 aacgccagaa cggttttctt caacgcccgc gcatcctggc gcgccgtttc agtccccatg
     4561 gtttgtaacg ccaggaaccg ctccactgta cgctgaaaat catcactgag ttgctgctga
     4621 aatcccgcca gcacgtccgg ttttatcccc ggccaatcgt cacgagcact aaaacaaggt
     4681 gacgacgcca cggtgaccag cgcctgaaca cgctcgggat gggttaacgc aatctggctt
     4741 gccaccagcc cgcccagact ccagcctaac caaatggctt tatcaggtgc ctgttgcagc
     4801 acggcttcgg ccatatcagc aagtgacagc gcaccaaatc cctggctgcg cccgaagccg
     4861 ggcagatcaa caaggtgcag cgtaaaatgc gagctaagtt cctcgtcaat gcaacgccac
     4921 acttcggcat tcagtcccca tccgtgcagc agcacaagat gaacattccc ctgacctttg
     4981 gtctgccacc agatgttatt catccgctat tgttctcttt tgacttacaa ggatgaacat
     5041 atgctaacag taccgggatt atgctggcta tgccgaatgc cgctggcgtt aggtcattgg
     5101 gggatttgtt cggtctgctc acgcgccgcc cgtacagata aaacgttatg cccacaatgt
     5161 ggattacccg ccacacactc acatcttccc tgcggtcgct gcctgcaaaa accaccgccc
     5221 tggcaaagac tggtcacggt tgccgactat gcgccgccgt taagtccgct tatccaccag
     5281 cttaaatttt cccggcgcag tgaaatcgcc agcgccctgt cacgtctgtt actattggaa
     5341 gtcttacacg ctcgtcgcac caccgggttg caattgccgg atcgcatcat cagcgttccg
     5401 ttatggcagc ggcgtcactg gcgtcgggga tttaatcaga gcgatttgct gtgtcagccg
     5461 ttatcacgct ggttgcactg ccgatgggat agcgaagccg tcacacgaac acgggccact
     5521 gcgacccagc attttctcag tgcccggcta cgcaagcgca acctgaaaaa tgcctttcgt
     5581 cttgaattgc ccgtgcaagg tcgccatatg gtgattgtgg atgatgtcgt taccaccgga
     5641 agtaccgtcg cagagattgc gcagttgctt ttacgcaatg gtgcggcgac tgtccaggtc
     5701 tggtgccttt gtcgaacctt gtagagcctc gatgataggc gtattataac caactaaaat
     5761 agtcaactat taggccatta ctatgatccg tatttccgat gctgcacaag cgcactttgc
     5821 caaactgctg gcaaaccagg aagaagggac acaaatccgc gtatttgtga ttaaccctgg
     5881 cacgcctaac gctgaatgtg gtgtttctta ttgtccgccg gatgccgtgg aagccaccga
     5941 cactgccctg aaatttgacc tgctgaccgc gtatgttgat gagttaagcg ctccttacct
     6001 ggaagatgca gagatcgatt ttgttaccga ccagttgggt tctcagctaa cactgaaagc
     6061 gcctaacgcc aaaatgcgta aagtggcaga cgatgcacca ctgatggagc gcgtggagta
     6121 tatgctgcag tcgcagatca acccacagct tgctggtcac ggtggccgcg tttcactgat
     6181 ggaaatcacc gaagacggtt acgccattct gcaatttggc ggcggctgta acggttgttc
     6241 catggtcgat gtgacgctga aagaagggat cgagaagcag ctgctaaacg aattcccgga
     6301 gctgaaaggt gtgcgcgatc tcaccgaaca ccagcgcggc gaacactcct actactaagt
     6361 tatcccctct tttgtagatt gcccgatgcg acgctaaagc gtcatttcgg gcctacgttg
     6421 aacatgtgcc agacgttggc gcagcattgc tttggcaaat ccctcccttc gtttctccgc
     6481 gataatatga ccaacctctc ataatttaaa tttactccgc tctggtgatt ctcaaacgcc
     6541 agatgttacc ggtatcattc acatgggtac caaacatact cctgacatct gactacaata
     6601 attagtttta gtgggtatca gtcgtggtgc cgcaatatct ctgttcccga ttgggataat
     6661 tagagtttgt cgtcagaaaa ttgacgttac ccataacaaa tgaaaggcca ggtaaatcat
     6721 gccattagtc attgttgcta tcggtgtaat cttgttgttg ctcctgatga tccgtttcaa
     6781 aatgaacggc ttcatcgctc tcgtcctcgt ggcgcttgct gttggattaa tgcaagggat
     6841 gccgctggat aaagttattg gctccatcaa agccggtgtc ggcgggacgc tcggtagcct
     6901 tgccctgatc atgggttttg gcgcgatgct gggcaaaatg ctggcagact gcggtggcgc
     6961 acaacgtatc gccaccaccc tgattgccaa atttggtaaa aaacacatcc agtgggcggt
     7021 ggtactgacc ggttttaccg ttggttttgc cctgttctat gaagtgggct ttgtgctgat
     7081 gctgccgctg gtgtttacca tcgcggcttc tgcgaatatt ccactgctgt atgttggtgt
     7141 accaatggca gctgcactgt ctgtgaccca cggcttcctg ccaccgcatc cgggtccgac
     7201 tgcgattgcc accattttca atgccgacat gggtaaaacc ctgctgtacg gtactattct
     7261 ggcaatcccg accgtgattc tcgccggtcc ggtttacgct cgcgtgttga aaggtatcga
     7321 taagccgatc cctgaaggtc tgtacagcgc gaaaaccttc agcgaagaag agatgccgag
     7381 ctttggcgtc agcgtctgga cctctctggt gccggtagtg ctgatggcga tgcgtgcgat
     7441 tgccgaaatg atcctgccga aaggtcacgc tttcctgccg gtagcggagt tcctcggtga
     7501 cccggtaatg gctacgctga ttgccgtgct gattgcgatg ttcacctttg gtctgaaccg
     7561 tggtcgttca atggatcaga ttaacgacac gctggtttct tccatcaaaa tcattgcgat
     7621 gatgctgttg atcatcggtg gtggcggtgc gttcaagcag gtgctggtag acagcggcgt
     7681 ggacaaatac attgcttcca tgatgcacga aaccaacatt tctccgctgc tgatggcctg
     7741 gtcgattgct gccgtactgc gtatcgcgct gggttccgca accgttgcgg caatcactgc
     7801 gggtggtatc gcggcaccgc tgattgcaac gacgggtgtt agcccggagc tgatggttat
     7861 tgcggtgggt tccggtagtg tgattttctc tcatgtgaac gatccgggct tctggctgtt
     7921 caaagagtac tttaacctga ctatcggcga gaccatcaaa tcctggtcga tgctggaaac
     7981 cattatctcg gtgtgcggtc tggtaggctg tctgctgctg aatatggtga tttgataaca
     8041 ctgactgccg gatgcatcgc atccggcatc aaccgcactc tactgcttct tcgctgcagc
     8101 tctgcgccgt ctgtccaaat ccttcagcaa cttattcacg ccatcatcgg caaacatcgt
     8161 ctcaagcgtt gcggaaagct tgcgtcgcca gtttttatac tggtaactgg tgccaggaat
     8221 attcaccggt tcggccatat ccagccagtc ttccggctgt agtcctaaca gagcactgtt
     8281 actgtcggca atgtagcgct gcaaaccacg attaagcgtc ggcgtcatcg acatcaacga
     8341 tgccttatgc ccggcacgtt ttggcagaca accatattta tgcagtgcat ccagcagccc
     8401 ttgcttcgcc agttcgcgat cctgatacag accgcgcagt accacttcat ccggatacag
     8461 ccccagggtt ttgcccaacg ttagatcccc gctctcccag taaccgcgca gcgtaggcag
     8521 gtcatgtgtc gccgcaaccg ccatcgactg ctccggatac gctttcggtg cacggaacgt
     8581 cttctcgtgg tcgttttcga aatagagcac tttgtaggag tacacaccgc tgctgcgcag
     8641 cttaccgaca atctctaccg gtacggtacc gagatcttca ccaatcacca tacagcgatg
     8701 acgtttactt tcgagtgcca gaatcgagag cagatcatcc accggatagt gaacatacgc
     8761 gccctgatct gccgtctcgc catacggtat ccaccacaaa cgcagcatcg acatcacatg
     8821 gtcaattcgt aatgcgccgc agttttgcat attggcacgc aacagctcga taaacggttc
     8881 ataggcacgc gcggtgatga tatgcgggtc cattggcggt aatccccagt tctgccccaa
     8941 cgggccgagg atatccggcg gcgcgccaac cgacgctttc aggcaatata gttcacggtc
     9001 acaccaggtt tccgccccac cttccgctac gccaaccgcc agatcacgat acaagccaat
     9061 cggcatttca tagccctggc ttatctccca gcaggcggca aactggctgt aagccagcca
     9121 ctgcaaccag agataaaaat cgacgtcatc acgatgttct tcgcagaact gacgcacttc
     9181 tggtgaatcc acgttctgat acatctctgg ccatgcaggc cagccccagc gcatttcgtc
     9241 ctctttcact tgctgggcat gtagcgcatc aaaggctgcc tgccagaaca ggctgtcgcc
     9301 ctgctctgca acaaactggc gaaacgcggt catctgctca tcatcacgtt gcgcgaaacc
     9361 tttccacgcc attcgtaatg ctgtcatttt tagggcggta accgtggagt aatcgaccca
     9421 gtcggcatcg cgcgcctgtt gcagcgtctg ttgcgtggtc ggcaactgcc accaggcctg
     9481 agcctcttcg ctaagatgga aatcttcaac ggcgttaacg tcgatataaa tcacattcag
     9541 ccaacggcga gaagacggac tgtatgggct ggcactctcc ggatttgccg gatagagcgc
     9601 atgaatcggg ttcaggccga tgaacgaccc gccacgtttt gccacatcca ccagcatcgc
     9661 tttgagatcg ccaaaatccc caatacccca gtttttttcc gatcgcagcg tataaagctg
     9721 aacgcaggca ccccacagct tttgtttatt cagcaacgcc tgcggttcgt aacagcgttt
     9781 cggggcgaca atcacccggc aatgcgcgcg ctggtcgtcc tgggtgagtg tcagcgtgtg
     9841 ataaccttcc ggcagcttcg tcggtagatt gaacgctttg ccccccgtta catggccttt
     9901 gtactgtgtt ccttcttcgg tggtcagcag ccagctatat tcgccgctgc cctccaccac
     9961 catcggcatt tttttgccgc tggtataaac catgacattc gggactggcg ttaccgccac
    10021 tttcgtggcg gtacgttgat gcatcgcgtc aagcaaacgc cgtttggttt cggcgctaat
    10081 cgactgcggt ttaccgtggg cattgatgta attggggcta atccccgccg ccagcgcggc
    10141 attatccaga cgtttgcttt ccatcgagct tccttagcgt tttgcctgcc agatacgagc
    10201 ctgataatcg cgaatagagc gatccgagct aaacataccg cagcgggcgg tattgaggat
    10261 cgccgcgcga gtccaggcct cttggtcgcg gtacagcaca tccacctgct tttgtgcctc
    10321 aacataggct gcgaaatccg ccatcaccag atacggatcg ccgccttgtt tgccgatact
    10381 gtgcagcatc tggtcgaagg catgcttatc gccgtcgctg tatttaccgc tttccagctc
    10441 tttcaatact gcgtccagca ccttatcttt cttccgccat ttcaccgggt cgtagccttt
    10501 ggccagaatt gccttcactt gttccacggt atgaccaaaa ataaagatat tttcttcacc
    10561 gactttctcg gcgatttcaa cgttcgcacc atccagcgta ccgacagtaa gcgcaccatt
    10621 gagcgccagt ttcatattgc cggtaccgga agcttcttta cctgcagtcg aaatttgttc
    10681 ggagatatcc gccgccggga tcagtttttc cgccgccgaa acgcaataat cctgcaggaa
    10741 caccaccttc aacttatcgc caaccagtgg atcgttgttg atcacgtcag ccactttgtt
    10801 gatcgcaaag ataatattct tcgccaggta gtagcccggt gccgctttcg cgccgaagag
    10861 gaagacgcgc ggtacgcgat cagcctgcgg gttttcacga atttctttgt acaacgccag
    10921 aatatgcagc agattcaggt gctggcgttt gtactcatgc agacgtttaa tctgaatatc
    10981 gaaaatcgcc tgtggattga tctcaatacc ggtacgaact ttcacaaact ccgccagacg
    11041 gactttattc gcctgcttga tctcgcgata ttgctgacgg aatttcgcat catcagcgaa
    11101 tttttccaga ttgatcagct gatcgagatc gttagcccac tctttttgca gtaatttatc
    11161 caacagagcc gccagtgcag ggttgcactg tttgatccag cgacgtgggg taataccgtt
    11221 ggtgacgtta tggaatttgt tcggccatag ctggtgatat tccgggaaca gatctttcac
    11281 caccagatcc gagtgcagcg ccgcaacacc gttcaccgcg aaaccgccaa ccacacacag
    11341 gttcgccata tgcacttgtt tatcgtgcac caccgccagt ttggcccaca ctttttcatc
    11401 gcccggccag gttttctcca ccagcgtttt aaagcgagta ttaatttcgt taataatctg
    11461 catatggcgc ggcagtaagc ctttcaccag tttcacatcc cagcgttcca gcgcttctgg
    11521 catcagggta tggttggtgt aagcgaaagt tttgctggta atggcccagg cgtcatccca
    11581 gctcatctgg tgctcatcga tcagcacgcg cagcagttct ggaatcgcga tagttgggtg
    11641 ggtatcgttc agctgaataa cttcataatc cgccagttcg tgcagtttac gccccgccag
    11701 atgatggcga cgcaaaatat ccgctaccga acaggcacac tggaagtact gctgcatcaa
    11761 gcgcagcttt ttaccggcag tatggttgtc gtttggatag agaactttgg tcagtttttc
    11821 cgcattgatg ccctgctgtt cggcacgcag gaaatcaccg tcgttaaatt tagtcagatc
    11881 aaacggatgc gcgtgcgtcg cctgccacag acgcagcggc tgcgccacgc cattacgata
    11941 gccgacaacg gggagatccc acgcttgacc ggtaatggta aactccggct cccagcgtcc
    12001 gtctttcgtc actttaccgc caatccctac ctgcacatcc agtgcttcgt tgtggcggaa
    12061 ccacgggtag ttactgcgat gccagtcatc cggcgcttca acctgtttgc catcgacaaa
    12121 agactggcgg aacaaaccat attgatagtt cagaccgtaa cccgtcgcag actgaccgat
    12181 agttgccatt gagtcgagga agcacgccgc cagacgtccc agaccaccgt tacccagcgc
    12241 cgggtcgatc tcttcttcca gcaggtccgt cagattgatg tcataagcct tcaacgaatc
    12301 ctgtacttcc tgataccagc cgagattcaa caggttgttg cccgtcaggc gaccaatcaa
    12361 aaactccatt gagatgtagt taacatgtcg ctgattcgcc accggcttgg cgaatggctg
    12421 agcacgcagc atttcggcca gtgcttcact cactgctagc caccactggc gaggagtcat
    12481 ttcagccgca gaatttaagc cataacgctg ccactgacgt gaaagcgctt cctgaaattg
    12541 cttatcgtta aaaataggtt gtgacatagg agttccactt ttcttagatt ttcaacacaa
    12601 cgttatcgct agtttgccag gctcgatgtt gaccttcctc atcctgcggg ggattaggca
    12661 gggaggagtt gcggggatga gcaaggaaat gtgatctcaa ccacttaaag ctagtgcaaa
    12721 ccacaggatt agcagcaaat caatgcaata cagcgcagaa aatctgtatc taagtgcaaa
    12781 aaatggccgt tgcgtatttt caaaaagcgg aaggtaactc tataaattaa gtaaaggagt
    12841 gaaacagttt cacaagtaaa atatccagtg tgctccatct cattcttaat agatttatta
    12901 agatcatctt tttagatggc actttcatca ggaatgaaga agaaaccctt gcttaaatga
    12961 atctgatgaa cataagggaa accagtattc acgctggatc agcgtcgttt taggtgagtt
    13021 gttaataaag atttggaatt gtgacacagt gcaaattcag acacataaaa aaacgtcatc
    13081 gcttgcatta gaaaggtttc tggccgacct tataaccatt aattacgaag cgcaaaaaaa
    13141 ataatatttc ctcattttcc acagtgaagt gattaactat gctgattccg tcaaaactaa
    13201 gtcgtccggt tcgactcgac cataccgtgg ttcgtgagcg cctgctggct aaactttccg
    13261 gcgcgaacaa cttccggctg gcgctgatca cgagtcctgc gggctacgga aagaccaccc
    13321 tcatttccca gtgggcggca ggcaaaaacg atatcggctg gtactcgctg gatgaaggtg
    13381 ataaccagca agagcgtttc gccagctatc tcattgccac cgtgcagcag gcaaccaacg
    13441 gtcactgtgc gatatgtgag acgatggcgc aaaaacggca atatgccagc ctgacgtcac
    13501 tcttcgccca gcttttcatt gagctggcgg aatggcatag cccactttat ctggtcatcg
    13561 atgactatca tctgatcact aatccagtga tccacgagtc aatgcgcttc tttattcgcc
    13621 atcaaccaga aaatctcacc ctggtggtgt tgtcacgcaa ccttccgcaa ctgggcattg
    13681 ccaatctgcg tgttcgtgac caactgctgg aaattggcag tcagcaactg gcatttaccc
    13741 atcaggaagc gaagcagttt tttgattgcc gtctgtcatc gccgattgaa gccgcagaaa
    13801 gcagtcggat ttgcgatgac gtttccggtt gggcgacggc actacagcta atcgccctct
    13861 ccgcccggca gaatacccac tcagcccata agtcggcacg ccgcctggcg ggaatcaatg
    13921 ccagccatct ttcggattat ctggtcgatg aggttttgga taacgtcgat ctcgcaacgc
    13981 gccattttct gttgaaaagc gccattttgc gctcaatgaa cgatgcgctc atcacccgtg
    14041 tgaccggcga agaaaacggg caaatgcgtc tcgaagagat tgagcgtcag gggctgtttt
    14101 tacagcggat ggatgatacc ggcgagtggt tctgctatca cccgctgttt ggcaacttcc
    14161 tgcgccagcg ctgccagtgg gaactggcgg cggagctgcc ggaaatccac cgtgccgccg
    14221 cagaaagctg gatggcccag ggatttccca gcgaagcgat tcatcatgcg ctggcggcag
    14281 gcgatgcgct gatgctgcgc gatattctgc ttaatcacgc ctggagtttg ttcaaccata
    14341 gcgaactgtc gctgctggaa gagtcgctta aggccctgcc gtgggacagt ttgctggaaa
    14401 atccgcagtt agtgttattg caggcgtggc taatgcaaag ccaacatcgc tatggcgaag
    14461 ttaacaccct gctagcccgt gctgaacatg aaatcaagga catcagagaa ggcaccatgc
    14521 acgcagaatt taacgctctg cgcgcccagg tggcgattaa cgatggtaat ccggatgaag
    14581 cggaacggct ggcaaaactg gcactggaag agctgccacc gggctggttc tatagccgca
    14641 ttgtggcaac ctcggtgctg ggtgaagtgc tgcactgcaa aggcgaattg acccgctcac
    14701 tggcgctaat gcagcaaacc gaacagatgg cacgccagca cgacgtctgg cactacgcct
    14761 tatggagttt aatccagcaa agtgaaattc tgtttgccca agggttcctg caaaccgcgt
    14821 gggaaacaca ggaaaaagcg ttccagctga tcaacgagca gcatctggaa cagctgccga
    14881 tgcatgagtt tctggtgcgc atccgagcac aactgttatg ggcctgggca cggctggatg
    14941 aagccgaagc gtcggcgcgt agcgggattg aagtcttgtc gtcttatcag ccacagcaac
    15001 agcttcagtg cctggcaatg ttgattcaat gctcgctggc ccgtggtgat ttagataacg
    15061 cccgtagcca gctgaaccgt ctggaaaacc tgctggggaa tggcaaatat cacagcgact
    15121 ggatctctaa cgccaacaaa gtccgggtga tttactggca aatgaccggc gataaagccg
    15181 ccgctgccaa ctggttgcgt catacggcta aaccggagtt tgcgaacaac cacttcctgc
    15241 aaggtcaatg gcgcaacatt gcccgcgcac aaatcttgct gggcgagttt gaacctgcgg
    15301 aaattgttct cgaagaactc aatgaaaatg cccgcagtct gcggttgatg agcgatctca
    15361 accgtaacct gttgctgctt aatcaactgt actggcaggc cggacgtaaa agtgacgccc
    15421 agcgcgtgtt gctggacgca ttaaaactgg cgaatcgcac cggatttatc agtcattttg
    15481 tcatcgaagg cgaagcgatg gcgcaacaac tgcgccagct gattcaactc aatacgctgc
    15541 cggaactgga acagcatcgc gcgcagcgta ttctgcgaga aatcaatcaa catcatcggc
    15601 ataaattcgc ccatttcgat gagaatttcg ttgaacgtct gctaaatcat ccggaagtgc
    15661 cagaactgat ccgcaccagc ccgttgaccc aacgtgaatg gcaggtcctg gggctgatct
    15721 actccggtta cagcaatgag caaattgcgg gcgagctgga agtggcggca accaccatca
    15781 agacgcatat ccgcaatctg taccaaaaac tcggcgtcgc ccaccgtcag gccgcggtac
    15841 aacacgcgca gaaactgctg aagatgatgg gatatggggt gtgagtttag ccggataatg
    15901 cattggatcc ggtttacatc tttacatcat tcaatactca cccgcgttac gccatctatt
    15961 tctatcaaac caaaccgcac cggcaagaaa cgctccacca cggcgatatt ggtcagcaaa
    16021 tggcatgaag gatgggcgac cgtaaattct cccgcgcccg ccagcgccat cggtagcacc
    16081 agctggtcgg cgagatattc ccccactgcc gccgggcttg ccaggtagcg tttcacctct
    16141 ttcactaact gcgcggctac cacctcggca ctgacgcgct tttcaccgac gacgaaaaag
    16201 cgttcagtga tattttcact ttcgacttcc agcgagacgg tattacccgg cccctggtcg
    16261 cgcggcaggt tatgaatatt ctgttcatgc agggaaaaac tccccgccag tgtagcgatt
    16321 tcacgctcag caacatggcg cggcacacca gctaatagaa cttctccacg catctgcaca
    16381 atgttcccgc gctcgccaag ttgcaaggtg ttaaacgatg ccacgggcga gacttccgtt
    16441 gccaccacgc cgcctccggc aggataaaaa ccgtgacgta acagcgtggt ttgctgatga
    16501 attcctattt tcgccagcag cggctccagc acccggcgga taaaatccgc aggcggcgcc
    16561 gacgggttat cggtgcctcc gctcacttca acacgcgaag gtccatcggc aaaccacagc
    16621 gcgggcagca ccgtttgcag caccagcgta cagcttccgg cactaccgat agcaaagcgg
    16681 tactcgccgc cgcgcacggc gccaggccgg aagaccagac gctgcgaccc cagctccgcg
    16741 ccttccaccg ttgccctaca aatttccgca gccgctttta ccgcggtcag atgctggcgc
    16801 aacagccccg gtttcgcccg cccggcacga atgctggtga tggtaaatgg ctggccggtt
    16861 atcatcgaca ggctcagcgc cgagcgcaga atctgcccgc caccttcgcc ctgtgcgcca
    16921 tccagcgcaa tcatcctttt catcatttat ccttttacgc acaccacctg acgcagggta
    16981 tagataactt ccaccagatc gctttgtgcc gtcatcaccg catcaatatc tttatacgcc
    17041 atcgggattt cgtcgatcac ttcggcatct ttacggcatt ccacatgcgc ggtggcacga
    17101 atttgatctt ccacgctgaa cagttttttc gctttagttc ggctcattac ccgcccggca
    17161 ccgtggctgc acgaacagaa cgactcttca tttcccagcc cacggacgat aaagcttttt
    17221 gctcccatcg aaccgggaat aattccatat tgaccagcac gcgcagacac cgcgccttta
    17281 cgcgtcacgt agatctcttc accaaagtgc tgttcttttt gcacatagtt gtggtgacag
    17341 ttgatctctt ccatcgccag cgtttgtggc tgtctgaccg ttttctgcgt aatgctctgc
    17401 aatgccgtta ccacgttttc catcatcaca tcgcggttaa ggctggcaaa aagctgcgcc
    17461 caggccacgg ctttcaggta atcatcaaag tattccgtac cttccataaa gtacgccaga
    17521 tcacgcgacg gcaacgtctc aagcgtttcc tgcatctctt tttgtgccag atcgataaag
    17581 taagtcccga tggcgttacc aattccgcgt gaaccggagt gcagcataat ccacacctgg
    17641 tccgactcat caaggcagat tttaataaag tggttaccgg ttcccagcgt tcccaggtgt
    17701 ttatagttat tggtattcag gaaacgggga tatttttgcg ttaaccactg ataaccggct
    17761 tcaagctcag cccatttcgc atcgacgttg acaggcgggt tttcccatgc gcctttatca
    17821 cgtttacaac gcccggtagt acgcccgtgc ggcacggccg tttcaatcgc ctgacgcagc
    17881 tctgccaggt tttcaggcag gtcttccgcc gttaacgcgg tacgcagcgc gttcattcca
    17941 cagccaatat ccacgcccac cgccgccgga ataatcgccc ctttggtcgg gatcacgcta
    18001 ccaatggtgg aatctttacc caggtgtaca tcaggcatta ccgcaatatg tttgaaaata
    18061 aacggcatct tcgccgtatt aataagttgc tgacgcgcat cggcttctac cggcacgcct
    18121 ttggtccaca tttttaccgg ggcattttca gtggtcagta attcgtaatt cattttgttt
    18181 ttctcttttc gttgtttgct gtcctgataa ttgcaaccgt cgtgccagaa aattaaataa
    18241 gtaaattaat tttttaattc attgttttta aaaagattat atctttatgt ccgtaaccgg
    18301 agatttcccg caaagccgat ttaccgataa tgaaatatcg tcttttataa ggatatctaa
    18361 gatgcgtaaa acagtagctt ttggctttgt cggaacggtg ctggattatg ccgggcgcgg
    18421 cagtcagcgc tggtcaaaat ggcatccgac actctgttta tgccagcaag aatcgttggt
    18481 catcgatcga ctggaattat tgcatgacgc ccgctcgtgc tcgctatttg aaacgcttaa
    18541 acgcgatatc gccagcgttt cgccagaaac agaagtggtg agcattgaga ttgaactgca
    18601 taacccgtgg gatttcgaag aggtttacgc ctgcctgcat gatttcgccc gtggttacaa
    18661 gtttcagcca gaaaaagaag actatttaat tcacatcacc accggcaccc acgtcgcgca
    18721 gatttgctgg tttctgctgg cggaagcgcg ttatctaccc gcccggttga tacaaacttc
    18781 accaccgcgc aaaaaagaac aaccgcgcgg tgccggtgaa gtgacgatta tcgatctcga
    18841 tctaagccgt tataacgcca tcgccagccg ctttgccgag gaacgccagc aaacgcttga
    18901 ttttcttaag tccggcattg ccacgcgtaa cccccacttc aaccgcatga ttgagcagat
    18961 tgaaaaagtg gcgattaaat cccgcgcacc gatcctgctt aacggcccaa ccggtgcggg
    19021 aaagtcattt ctggcgcgac gtatatttga attaaaacag gcgcggcatc agtttagcgg
    19081 cgcgtttgtg gaagtgaact gcgccaccct gcgcggcgat accgccatgt cgacgctgtt
    19141 tggtcatgtg aaaggcgcgt ttaccggggc gcgggaatct cgtgaaggtt tattacgcag
    19201 cgccaacggc ggaatgttgt tttttgatga gattggcgaa ctgggcgcag acgaacaggc
    19261 aatgctgcta aaagccattg aagagaaaac cttttacccg tttggcagcg atcgccaggt
    19321 gagcagtgat tttcagctta tcgccggaac ggtgcgcgat ttgcgccagc tggttgccga
    19381 aggcaaattt cgcgaagatc tgtacgcgcg gatcaatctc tggaccttca ccctgccggg
    19441 tctacgccag cgccaggaag atattgaacc gaaccttgat tatgaagtgg agcgccacgc
    19501 cacactcact ggcgacagcg tgcgttttaa caccgaagcg cggcgcgcct ggttggcttt
    19561 tgcgacctct ccccaggcaa catggcgcgg taactttcgc gaactttctg ccagcgtcac
    19621 gcggatggcc acctttgcca ctagcggacg catcactctg gacgtggttg aagatgagat
    19681 aaaccgtctg cgctataact ggcaggagag ccgcccctcc gcgcttacgg cgttgctggg
    19741 cgcagaggca gaaaacatcg atctcttcga ccgtatgcaa ctggaacacg ttatcgctat
    19801 ctgccgccag gcaaagtcgc tttctgcaac cggacgacag ctttttgatg tttcgcgcca
    19861 gggaaaagcc agcgtcaatg acgcggatcg gctacgcaaa tacctggcgc gttttggtct
    19921 gacgtgggaa gccgtgcagg atcagcacag ctccagttga atatgatggt ccgtcagcac
    19981 ctgcattacg cttactggcg gcggggcgtc ggtgtagacg gcatctacca tgctgatgct
    20041 gcccatattg accatcgcgt tacggccaaa tttcgagtga tcgacaacca gcataacgtg
    20101 gcgcgagttc tcaataatgg cgcgcttggt gcgaacttcg tggtaatcga actccagcag
    20161 cgagccgtcg ctatcgatgc cgcttatccc cagaatgccg aaatcaaggc ggaactggga
    20221 gataaaatcg agcgtcgctt cgccaatgat cccgccatcg cggctgcgta attcgccacc
    20281 agcgagaata atgcggaaat cttcttttac catcaacgtg ttagcaacat tgagattgtt
    20341 ggtgacaata cgcaaattgc tgtgattaag cagcgcgtgc gccaccgctt ccggcgtggt
    20401 gccgatatcg ataaacagtg tcgagccgtt ggggatttgc tccgccactt tgcgggcaat
    20461 cctctctttt tcttcggtct gggtggcttt gcgatcgtgc cacggcgtgt taaccgaact
    20521 ggaaggcagc gccgcaccgc catggtgacg caggatcagg ttttgctccg ccagctcatt
    20581 gaggtcgcgg cgaatagtct gcgggctgac ggagaaatgc tctaccagct cttcagtact
    20641 gacataaccc tgctgtttaa ccagttcgat aataccgttg tgacgttgtg tttgtttcat
    20701 ttataaatcc ctggaattat tttcgttttc gcgcattgag cgaatcaaca aaagccatcg
    20761 ctaaacccac ggctaacccg gcgatgtgtg ctccgttcgc catcgacatc ccaaacaaat
    20821 caaaccatcc ggcgataatc cagatcagcg caaagataat taacccacgt tgcaggtaaa
    20881 tgccactttg cggatcgcgc tcaccacgta gccagacgta gcccatcagc gcatacacca
    20941 cgccagaaag cccgccaaac cacggcccgc tgaatttttg ctgcacatag ccgcttaaca
    21001 gggcactaat gagagtaata acaattagct taccgctacc gaggcgtttt tccaccgcac
    21061 cgccgagata ccaccaccag agcaggttaa agaggatatg catcagcgag aagtgcatta
    21121 acgcgtgggt gaagtaacgc cagaactcaa atttcagtgt tggatcgaat ggccaggcca
    21181 gccataacat cacttcctga tcgccgagaa tttgcatggc gataaacacc accacgcagg
    21241 cgatcatcac cacccaggtt accggacctg cgcgttcacg caaggcagca aagaaaggat
    21301 aacggcgata atgcagacca ctgccggtat ggcccgcctg ccagctggcc gccagataac
    21361 gcggatctgc cgggttttcg agaaaacgcg ccagctccgc ccgcacgcgc tcggcctggg
    21421 actcatccgc cagccagaca tcgctttggt tatgttgttg aatcgtaagg ataacaccct
    21481 gcgtcgccat gtaatcaaca aacgcctgcg ccacgcgggg gttagcaaaa gaggtaatca
    21541 tcaacatcgt tgctgtcgct tattccacac aaaaggggac agtataaagc gttacgcgcc
    21601 gtacgccacc tctgcgggaa actgacgctg ccaggcttca aaaccgccgt caatgctata
    21661 gaccacatcg tagccctgtt gcagcagata ctgcgccgcg cctttgctgc tattgccgtg
    21721 ataacacatc accatcaccg gagtgtcaaa gtcgttatca cgcataaacg cgcccagcgt
    21781 gtcgttggtt aaatggaaag cctgcaccgc atgtcccatt gcgaaactct gtggatcgcg
    21841 aatatcgacc agcaccgcct ctttttcctg caacttctgg tgcgcgtcgg caacgttaat
    21901 acattcgaac agatccatgc gtctctcttt ctttacaaac aagtgggcaa atttaccgca
    21961 cagtttacgt cgaagcggca gataaacgcc ataatgttat acatatcact ctaaaatgtt
    22021 ttttcaatgt tacctaaagc gcgattcttt gctaatatgt tcgataacga acatttatga
    22081 gctttaacga aagtgaatga gggcagcatg gaaaccaaag atctgattgt gataggtggc
    22141 ggcatcaatg gtgctggtat cgcggcagac gccgctggac gcggtttatc cgtgctgatg
    22201 ctggaggcgc aggatctcgc ttgcgcgacc tcttccgcca gttcaaaact cattcacggt
    22261 ggcctgcgct accttgagca ctatgaattc cgcctggtca gcgaggcgct ggctgaacgt
    22321 gaagtgctgc tgaaaatggc tccgcatatc gccttcccaa tgcgttttcg cctgccacat
    22381 cgtccgcatc tgcgcccggc gtggatgatt cggattggtc tgtttatgta cgatcatctg
    22441 ggtaaacgca ccagcctgcc gggatcaacc ggtttgcgtt ttggcgcaaa ctcagtgtta
    22501 aaaccggaaa ttaagcgcgg attcgaatat tctgactgtt gggtagacga cgcccgtctg
    22561 gtactcgcca acgcccagat ggtggtgcgt aaaggcggcg aagtgcttac tcggactcgc
    22621 gccacctctg ctcgccgcga aaacggcctg tggattgtgg aagcggaaga tatcgatacc
    22681 ggcaaaaaat atagctggca agcgcgcggc ctggttaacg ccaccggccc gtgggtgaaa
    22741 cagttcttcg acgaggggat gcatctgcct tcgccttatg gcattcgcct gatcaaaggc
    22801 agccatattg tggtgccgcg tgtgcatacc cagaagcagg cctacattct gcaaaacgaa
    22861 gataaacgta ttgtgttcgt gatcccgtgg atggacgaat tttccatcat cggcaccacc
    22921 gacgtagagt ataaaggcga tccgaaagcg gtgaagattg aagagagtga aatcaactat
    22981 ctgctgaaag tgtataacgc gcactttaaa aaacagctta gccgtgacga tatcgtctgg
    23041 acctactccg gtgtgcgtcc gctgtgtgat gatgagtccg actcgccgca ggctattacc
    23101 cgtgattaca cccttgatat ccatgatgaa aacggcaaag caccgctgct gtcggtattc
    23161 ggcggtaagc tgaccaccta ccgaaaactg gcagaacatg cgctggaaaa actgacgccg
    23221 tattatcagg gtattggccc ggcgtggacg aaagagagcg tgctgccggg tggtgccatt
    23281 gaaggcgacc gcgacgatta cgcagctcgt ctgcgccgtc gctatccatt cctgactgaa
    23341 tcgctggcgc gtcattacgc acgcacttac ggcagcaaca gcgagctgct gctcggcaat
    23401 gcgggggcga taagcgacct cggggaggat ttcggtcatg agttctacga agcggaactg
    23461 aaatacctgg tggatcacga atgggtccgc cgcaccgacg atgccctttg gcgtcgcaca
    23521 aaacaaggca tgtggctgaa tgcggatcaa caatctcgtg tgagtcagtg gctggtggaa
    23581 tatacgcagc agaagttgtc gctggcgtcg taaggatgat gcccggttga agtgcaaccg
    23641 ggctatttgg attccttaga ctattcattg taaataattg aataacggcc cctgagctaa
    23701 aatcgccaaa cttaagcacg ttacccaatc gccccacttc ttcctctggt ttctattttt
    23761 tctgcaccaa tggcaataat atcccaatgc aaaaccaagc tgtgcactaa tcgggccgac
    23821 aaaatcagtc caaggctgcg gccaaaaagg ggaaaaattc aggggaggcc agacaaacgt
    23881 cagaaagaaa tttcggatat tttccagcaa cgctgatttc tctggagaaa tataaagaat
    23941 gaaattcgag agaattaacc agaaatagac cggcgaaaag accagccagc aatgcagtct
    24001 gttttttaat tcaggaggcg ttctgcggga gaattgccaa ccgatgccag ccattacact
//
LOCUS       DAZPEB010000042        23521 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZPEB010000042 DAZPEB010000000
VERSION     DAZPEB010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23521
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.043"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(82..2301)
                     /locus_tag="ACHURJ_001502"
     CDS             complement(82..2301)
                     /locus_tag="ACHURJ_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFR1280505.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSSEEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVLHPAYGNVRIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(2412..3824)
                     /gene="ftsP"
                     /locus_tag="ACHURJ_001503"
     CDS             complement(2412..3824)
                     /gene="ftsP"
                     /locus_tag="ACHURJ_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFR1280506.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDSRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFLEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(3899..4636)
                     /gene="plsC"
                     /locus_tag="ACHURJ_001504"
                     /gene_synonym="parF"
     CDS             complement(3899..4636)
                     /gene="plsC"
                     /locus_tag="ACHURJ_001504"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFR1280507.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(4869..7127)
                     /gene="parC"
                     /locus_tag="ACHURJ_001505"
     CDS             complement(4869..7127)
                     /gene="parC"
                     /locus_tag="ACHURJ_001505"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFR1280508.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LVSERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(7673..8155)
                     /locus_tag="ACHURJ_001506"
     CDS             complement(7673..8155)
                     /locus_tag="ACHURJ_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFR1280509.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPKAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTVWGIIRHWLPASGEKMRKAPILFHYTNLAEGMTEQRLETDVYVPLA
                     "
     gene            complement(8208..8600)
                     /gene="ygiW"
                     /locus_tag="ACHURJ_001507"
     CDS             complement(8208..8600)
                     /gene="ygiW"
                     /locus_tag="ACHURJ_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFR1280510.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSRRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            8752..9411
                     /gene="qseB"
                     /locus_tag="ACHURJ_001508"
     CDS             8752..9411
                     /gene="qseB"
                     /locus_tag="ACHURJ_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFR1280511.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLASEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            9408..10757
                     /gene="qseC"
                     /locus_tag="ACHURJ_001509"
     CDS             9408..10757
                     /gene="qseC"
                     /locus_tag="ACHURJ_001509"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFR1280512.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDS
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNVQGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPSGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            10867..11448
                     /gene="mdaB"
                     /locus_tag="ACHURJ_001510"
     CDS             10867..11448
                     /gene="mdaB"
                     /locus_tag="ACHURJ_001510"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFR1280513.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            11479..11793
                     /locus_tag="ACHURJ_001511"
     CDS             11479..11793
                     /locus_tag="ACHURJ_001511"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFR1280514.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(11841..13733)
                     /gene="parE"
                     /locus_tag="ACHURJ_001512"
     CDS             complement(11841..13733)
                     /gene="parE"
                     /locus_tag="ACHURJ_001512"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFR1280515.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQIYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(13762..14343)
                     /gene="yqiA"
                     /locus_tag="ACHURJ_001513"
     CDS             complement(13762..14343)
                     /gene="yqiA"
                     /locus_tag="ACHURJ_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFR1280516.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(14343..15170)
                     /gene="cpdA"
                     /locus_tag="ACHURJ_001514"
     CDS             complement(14343..15170)
                     /gene="cpdA"
                     /locus_tag="ACHURJ_001514"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFR1280517.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(15195..15617)
                     /locus_tag="ACHURJ_001515"
     CDS             complement(15195..15617)
                     /locus_tag="ACHURJ_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFR1280518.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTTVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(15618..16247)
                     /gene="nudF"
                     /locus_tag="ACHURJ_001516"
     CDS             complement(15618..16247)
                     /gene="nudF"
                     /locus_tag="ACHURJ_001516"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFR1280519.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHSLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            16452..17933
                     /gene="tolC"
                     /locus_tag="ACHURJ_001517"
     CDS             16452..17933
                     /gene="tolC"
                     /locus_tag="ACHURJ_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFR1280520.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSIVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            18081..18752
                     /locus_tag="ACHURJ_001518"
     CDS             18081..18752
                     /locus_tag="ACHURJ_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFR1280521.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLASCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            18758..19918
                     /locus_tag="ACHURJ_001519"
     CDS             18758..19918
                     /locus_tag="ACHURJ_001519"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFR1280522.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLPLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(19956..20744)
                     /gene="ygiD"
                     /locus_tag="ACHURJ_001520"
     CDS             complement(19956..20744)
                     /gene="ygiD"
                     /locus_tag="ACHURJ_001520"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFR1280523.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLAPVPVT
                     LDKEARGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            20887..21648
                     /gene="zupT"
                     /locus_tag="ACHURJ_001521"
     CDS             20887..21648
                     /gene="zupT"
                     /locus_tag="ACHURJ_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFR1280524.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGISPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAVAGIMVALSVDELMP
                     LAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(21706..21876)
                     /gene="yqiD"
                     /locus_tag="ACHURJ_001522"
     CDS             complement(21706..21876)
                     /gene="yqiD"
                     /locus_tag="ACHURJ_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFR1280525.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(22136..22789)
                     /gene="ribB"
                     /locus_tag="ACHURJ_001523"
     CDS             complement(22136..22789)
                     /gene="ribB"
                     /locus_tag="ACHURJ_001523"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFR1280526.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(22893..23041)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            23163..23453
                     /gene="ubiK"
                     /locus_tag="ACHURJ_001524"
     CDS             23163..23453
                     /gene="ubiK"
                     /locus_tag="ACHURJ_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFR1280527.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRMSELENRSTEIKKQPDPETLPPTL"
BASE COUNT         5749 a         6335 c         5930 g         5507 t
ORIGIN      
        1 tcggcatata aataaatgcc taatgagata gattctgatg taatatcccc ggtagcaatt
       61 ctgctaccgg gaacagcgaa tttaacgtcc aaccgccgct ttaggccgtt tcttcgcacc
      121 agcattcacc ggacgagatt gcgtagacga cgcttttttt gccgtagcag gcgtctgacg
      181 ctgggtcgcc atcggcgtat gtttcgtcaa cgccggacgg gtattgcggt tctggcgacg
      241 agcttcacgc atctcttcaa tggttggcgc aggcactaag caatcgcgac ggctaccaat
      301 cagatgcttt ttacccatcg cttccagcgc ctggcggatt aacggccagt ttgccggatc
      361 gtggtaacgc aacaacgctt tatgcaaacg acgctgcttg tcgcccttcg gtacgaagac
      421 gtcttcactc ttataaccaa tcttcgccag cggttttttc ccggtgtaat acatggtggt
      481 tgagttcgcc agcggcgatg ggtagaagtt ctgtacctgg tcgagacgga aacgatgctt
      541 tttcagccac agcgccagat tcaccatatc ttcatcacgc gtaccggggt gcgcggagat
      601 gaaatagggg atcagatact gctctttacc tgcctgtttt gagtaagtat cgaacagctc
      661 tttaaagcgg tcatagctgc ccatgcccgg cttcatcatc ttcgataacg gcccttcttc
      721 ggtatgttcc ggggcaatct tcagataacc gccgacgtga tgggtcgcca gttctttgat
      781 atagcgcgga tcttccacgg cgatgtcata acgcacacca gaggcaatca ggatcttttt
      841 aatgcctttc agatcacgcg cacggcgata gaggttgatc gttggttcgt ggttcgtgtc
      901 catgtgcgga caaatatccg gataaacgca cgacaaacgg cggcaggttt gctcagcgcg
      961 tggcgatttg cagcgcaaca tatacatgtt ggcagttggc ccaccgagat cggaaattac
     1021 gcccgtaaaa cctggaacgg tgtcgcggat cgcttcgatc tcattaatga tcgaatcttc
     1081 ggaacggctc tgaataatgc gcccttcgtg ctcggtgata gaacagaaag agcagccgcc
     1141 aaagcagcca cgcataatgt tgaccgaaaa acggatcatt tcgtaagccg gaatacggac
     1201 attgccatag gccggatgta gcacgcgctt gtatggcagc gcaaaaacgc tgtccatctc
     1261 ttcggtagaa agcggaatag caggcgggtt gatccacaca tagcggtcgc cgtgtttttg
     1321 catcaatgcg cgggcacagc ctgggttggt ttcgtggtgc agaatacgcg aagcatgggc
     1381 gtacagcact ttatcgccct tcactttctc gaaagaaggc agcaacacgt aggttttttc
     1441 ccacggtttc gggcgcggtg gctgcacggt tacggctttg gcttcctgct ttttcggtgc
     1501 caccggtttg ttatccgcgc acggcaaatc ttcaccatac ggatgcggga ttgggtcgat
     1561 ttttccaggg gtatcaagac gggtggaatc cacgccgctc cagccaggca gggcctcttt
     1621 cacgataatc gcggtattac gcacatcgcg gatttcacta atcggctcgc ccatcgccag
     1681 acggtgcgcc acctccacca gcggacgctc accgttacca aacatcagca tgtcggcttt
     1741 cgaatccacc agcacggaac ggcgcacggt atcggaccag taatcataat gtgcggtacg
     1801 acgcagacta gcctcaatac cgccgaggat caccggtaca tctttccacg cctctttaca
     1861 acgctgggta taaaccagtg tggcgcgatc cgggcgctta cccgcgacgt tatccggcgt
     1921 gtaggcatcg tcatgacgta aacggcgatc ggcggtataa cggttgatca tcgaatccat
     1981 gttgccagca gtaacaccga aaaacagatt cggtttaccc agacgcataa agtcgtcttt
     2041 gctgctccag tccggctggg cgatgatccc gacgcgaaag ccctgcgctt ccagcatacg
     2101 accgcaaatc gccatcccga agcttgggtg atcgacatac gcgtcgccag taaccaaaat
     2161 gatgtcgcag ctatcccagc caagttgatc catctcttca ctagacatcg gcaaaaacgg
     2221 tgccggtcca aaacaggcgg cccagtactg cggccaggag aacaggtcgc gatccggttg
     2281 gatcagggag atagagctca ttttgcttcc agaaatgata aaaaaataat caaaggccgg
     2341 ggattataag ccggaacgaa agagaaatcg aaaagtattc catactcgcc ctcctcgggc
     2401 gagtatgaag attacggtac cggattgact aacagttgcc caatcgagcc acggtccgcc
     2461 atttccagcg tctgactgtt gaagtagaac gggaagtgcg cccaggaagg ctgaccgaaa
     2521 taaacaagca gctccacttg tccgtcaacc caaacggtat ctttccagcc tctgtcttcc
     2581 aggaacggca tcgcaccatt cacgttacgg atctggaaca tcacgccttc aatatggaac
     2641 gcttgcggct cgtccgcgcg taccgtccag cgttcccacg ttccttgctg cgcggtgaca
     2701 tcaatacggt tgacgtccca cagttgtcca ttaatacccg ggtcatcacc cagactgata
     2761 tcgcgactgc gaatcggcga accggccatg atttcagttg gcagcaagcg catcggaaga
     2821 ctgtctgtga ccagcggcag aaggccggtt gggcgcagcg ttagcaccag ggtagaaacc
     2881 agaatgctgg atggctcaaa gaagccacga atacgatcaa caatgctcgc cgcttcgcca
     2941 caggtgatcg acacttcatc accgttgctc atatccacca gaatctcgcg gcgctcgccc
     3001 ggtgccaacg aaagttgctt caccgataca ggagcaggca ggaatccctg atcgccagaa
     3061 atcacatgta acgggcgact atcgctcatc tgtagttgat agcgacgaga gttcgacgcg
     3121 ttcagcagtc gcaagcgcac ccagccacgc gagacttcaa cgtacgggct ttgtacaccg
     3181 ttaaccagca gcgtatcacc aacaaagccg ccgcttcccg gttcgttgta ttctggcgta
     3241 ccaaagttat ccagccgttt atcctggata atgaccggaa aatcatccac accataatgg
     3301 ttggggatag gcagcgactt gctgacttca tcttccacca gccacattcc ggcaaggccg
     3361 ttatagacct gctgagccgt gcggttggga gtattggcgt gataccacag agtagctgcg
     3421 ttctggcgaa tgggcagtac gggtgcccag tcagcgtttg gcgacatcat ccgtgccgga
     3481 ccgcccatca gcgggcctgg tacctgtagc ccggccaccg tcattgagac attttctgtc
     3541 aggcggttgc tgtaaataag cttaacatcg tcgcccttcc agacgcggat agtcggcccc
     3601 aggtaacgac cattgattcc ccagaccgac gcgcgtgtcc ctggcgtaaa tgaccagtgc
     3661 gcacgttgta cagtcataaa cagcggttgc ccacggcgag attcaagtag cggcggaacg
     3721 ggtagcggtt gctgttgccc ggctgcgctg gccttcaggg gaacagcgcc ggcacaaagt
     3781 gcaatccccg atgcctgaat gaactgacgc cgactgagtg acatatttgc tccatgtaaa
     3841 actggctaaa gtaataccgt gcaggaaagt gttccccgca aaaatacaga ttgccgactt
     3901 aaacttttcc ggcggcttcg cgttctgcga cttctttatc gagctcggcg attttttgtt
     3961 ccattatcga acgacaatgg gcagccagct cacgaacctg atctttgcca tactgactga
     4021 cgtcaattgg cggcagcatt tcgacaatca ccagaccgtt gtgcagtcga ttaagattaa
     4081 tcttattcga agttgtagag acgcacacgg gaataatcgg gacgcccgcc gcaattgccg
     4141 cgtgaaatgc tccagtcttg aacggtagca ggccgcgacc acggctgcgg gttccttccg
     4201 ggaacatcca gatggaaata cggcgttttt tgaaatgatt cacgacttcc gcaatggtgc
     4261 cgtgagcttt agtgcgattg tttctgtcga tcaataagtt gccggttaac cagtacaact
     4321 gcccgaagaa ggggatccac agcaagctct ttttacctac cgtcaccgtc ggcggttgca
     4381 caatgttcga tgctgtcacc atgtcatagt tgttctggtg gttagcgata tagatagcat
     4441 tgccgtagct ttcagcatct gcaggtttgc ggcactcaac tttcaggccg aacagcggcg
     4501 caagacggcc aaacatatgc ccaaaggtgg ccacatgttt cgggttacgc gggctgaaaa
     4561 ggcagtaaat ggagccgaat acacagacta agatgctgta aatcacggta ataataagac
     4621 gaaagatata tagcataaca acctctgaag gtctgagaga ctggcattgt acgtcacctg
     4681 aaatcaggtt agggcattgt taaaagccgc tctctggaaa agcgtattgt taatcgcaac
     4741 gcatgaataa acaacggttt caagggaatt tttatcgttt ccgggggttg aatacgctgc
     4801 cggatgacga cttaacgttt catccggcgt tccttgcaag cgggaggaaa caggccctcc
     4861 ccggcatatt actcttcgct atcaccgctg ctggcacggc gaggagagtc gatctcaaca
     4921 cgatcgatac gctgcaaacc gcgcatcaac gtaccgcggc gtccacgttc gccagtgact
     4981 ttctgtaact cttccgggcg cagtttaatt ttgcgtttcc caacatgaat ggtcagcgtg
     5041 ctttgcggcg gcagaacgta caattgcgcc agaccatctt ctccacgcgc ggcttctgcc
     5101 gatggaatgt tgataatctt gttgcctttg cccttcgaca gctgcggcag atcgcttacc
     5161 gggaacatca acatacggcc tgcctgagtg attgccagca gcatatcgga agcatcttca
     5221 atcaccaccg gcggcataac atgggcattt tccggtaagg tgatcaaagc cttacctgca
     5281 cggttacgcg ccaccagatc gttaaaggtg cagacgaaac cgtaacccgc atcggaagcc
     5341 atcagcagtt tctgatcgtc gctttccatc agcatatggt caacggtcgc cccaggcggc
     5401 aacgttaatt tgccggtgag tggttcgccc tgaccacgcg ccgacggcag cgtaatcggg
     5461 tcgatggcat agctacgacc ggtggaatca acaaacacta ccggttggtt gctcttacct
     5521 ttcaccgccg ctttgaagct atcacccgct ttataattca ggcccggcgc gtcgatatca
     5581 tggcctttag cgctgcgtac ccagcccatc tgcgacagca caatggtgac aggttcagac
     5641 ggcagcatgt cgtgctcgct catcgctttc gcttcttcgc gttcctgcaa cggcgaacga
     5701 cgatcgtcac cgtaggcttg cgcgtctgcc tgcagttctt tcttcagcag gttattcatt
     5761 ttacgctcgg aaaccaaaat gccctgcaac tggtcgcgct ctttttccag ctcactctgc
     5821 tcaccgcgaa tcttcatctc ttccagtttg gcaagatgac gcagtttcag ttcgaggatc
     5881 gcttccgcct gggtttccgt aaggccaaac cgcgacatca gcgccggttt cggttcatct
     5941 tcgttacgaa tgatctcaat cacttcgtcg atgttgagaa acgccaccag caaaccttcc
     6001 aggatatgca ggcgcttgag gactttctcc agacgatagt tcagtcggcg gcgcacggta
     6061 tcgcgacgga acaccagcca ttcggagagg atttccagca ggtttttcac cgcaggacga
     6121 ccatccagac cgatcatatt gaggttaatg cgatagctct tttccagatc ggtggtagcg
     6181 aagaggtggt tcatcacctg atccatatcc acgcggttgg aacgcggcac aatcaccaga
     6241 cgggtcgggt tctcgtggtc agattcatcg cgcagatcgt caaccatcgg cagctttttg
     6301 ttgcgcattt gcgcagcaat ttgctccagt acgcgcgcac ctgaaacctg atgcggcaat
     6361 gcgctgataa ccaccgcgcc atcttctttc ttccacaccg cgcgcatacg cactgaacca
     6421 cgtccgttct cgtagatttt acggatctcg gcgcgcgaag tgataatttc cgcttcagtc
     6481 ggataatccg gcccctgcac gatatccagc agctgatcga gcgtggtttt cggctggtcg
     6541 attaatgcga ttgccgcctg agctacttca cgcaggttat gcggtggaat atcggtcgcc
     6601 atgccgacgg caataccggt ggtgccgtta agcaaaatgt ttggcagacg ggcaggcagc
     6661 attttcggct cctgcaaagt gccgtcgaag tttggcaccc agtcagccgt cccctgcccc
     6721 agctcgctca atagcagctc ggaatatttc gacaaccggg attcggtgta acgcattgcc
     6781 gcgaacgatt tcggatcgtc cggcgcgccc cagttcccct gaccatcaac cagcggataa
     6841 cggtaagaga acggctgcgc catcaggacc atcgcttcat aacaggcgct atcgccgtgc
     6901 ggatggtatt tacccagtac gtcaccgacg gtacgggccg attttttaaa tttggcgctg
     6961 gcattcaggc ccagttcaga catcgcatac acaatgcggc gctgaacagg tttaagacca
     7021 tcgccaataa acggcaacgc acggtccatg atcacgtaca tggagtagtt taagtaggcg
     7081 ttttccgtaa attcatgtag cgcaaggcgc tctgccatat cgctcattaa ttctgattcc
     7141 tcaacttatt cgccagcctt caaaggggca atattgccgg agatactaca ttatctgccg
     7201 cgtcgagtca caggagatca gcgtgaatag cgccgccgga cgtgctccga cggcgataaa
     7261 ggcatcaatg tgccttgata tacaactctt tgctgtagac ttgatcggca tagagggcag
     7321 cacaaggttg cgcccatgcc acaccactcg gcatgggggt ccgctccggg cgattcgaga
     7381 agttgaggtt atgcgagtcc agggaagcct aaagcatcaa agtagtcgac tgtaagtata
     7441 cggttcagcc cagtttagct gtgtctccac cagtgccccg cgcctcctgc cataagtgcg
     7501 gcttgctcat ggataccccc gagacggctt acggcgcacc ggataataaa tttcgcgtat
     7561 acatggtatt ccttaccgga gaaattataa gcccaacaac aatacgcctg tggcattgtt
     7621 cgctcaaact tatcgcgagt gatttggctc acactccggt aaagattccc catcacgcta
     7681 acggcacata aacatccgtt tccagtcgct gttctgtcat accttcggca agattggtgt
     7741 agtgaaacag aatcggtgct ttacgcattt tctcgccgct tgcaggcagc cagtggcgaa
     7801 tgatgcccca taccgtgtgt gaaatatcgt ccagctcgcc aacgtggcgg gccacggcat
     7861 aacgtccacc ggtaagttca ccattgctca caccataacg attatcggga atcggttcgc
     7921 taacgctgcc gcagatatca aagcgaaacg ctttcggtgc ggtggtggca gggtcgtccc
     7981 aggcgacgcc aaaagtctgg ctttggttaa caggagaaag ccccgtctct ttacgccaca
     8041 tgataaattt cgccacactg tagttgagca actcagggct acagcggtgc ggcaacatcg
     8101 ccacagggat tgatgggaaa tcgataatgt ttacatccag tgtcaggttt gtcattttgc
     8161 ctccctgcga aattattccc gggaacgcgt cccgggagcg gtaacaatta cggatttact
     8221 ttgcggatct gtttgacgtc aatttcaaca gaattccagt ctttatcgac ttccccctga
     8281 atctcaaccg tatctttcgg cgtcaccgtc acaccgttcc agcgtttgtg gtcgatatca
     8341 acattgatag taccgctggc atctttaaac acgtagagat cgtcagagat gcgttcaacg
     8401 atattgccgc gcagggttac ccaggtgtcg tcacgccggg attttgcgct ttctacagtc
     8461 gttacgctgc cgttcggccc ctggaatcct ccggcctgac tttgcgttgc cgatgggcca
     8521 gaaaaaccgc cctgctctgc tgccatcacc ggtgcactgc acagggccat tactgcgatt
     8581 actgctgcga attttttcat gtttattact ccctttaatg tctgtttccg agcatttaac
     8641 aagatagtcc ttaacaactt cttaagggaa aaaaataaaa tttaatgctg tacagagcgc
     8701 gttacaacac ggtttactgg cagcaaatac ggttatcgca gggatgaaaa aatgcgaatt
     8761 ttactgatag aagatgacat gctgattggc gacggcatca aaactggcct tagtaaaatg
     8821 ggttttagtg tcgactggtt tacacaaggt cgtcagggaa aagaggcgct ttatagcgca
     8881 ccttatgatg cggtgatcct ggatttaacc ttaccaggca tggatggtcg cgatattttg
     8941 cgcgaatggc gagagaaagg tcagcgtgag ccggtactga tcctgaccgc gcgtgatgcg
     9001 ttagcggaac gtgtagaagg gctgcgtctg ggagctgacg attatctgtg taaacctttt
     9061 gcgttgatag aagtcgccgc caggctggag gctctgatgc gccgaaccaa cggccaggcc
     9121 agcaacgagc tgcgccacgg taacgtcatg ctcgaccccg gcaaacgtat cgccacgctg
     9181 gctagcgaac ccttaacact gaaaccaaaa gaatttgccc tgctggaatt actgatgcgt
     9241 aacgctggtc gggtactgcc gcgcaaactg attgaagaga aactgtatac ctgggacgaa
     9301 gaggtcacca gtaatgccgt tgaagtgcat gtgcatcatc tgcgacgcaa actcggtagt
     9361 gattttattc gtaccgtgca tggtattggc tacacattag gtgagaaatg aaatttaccc
     9421 aacgtcttag tctgcgcgtc aggctgacgc taatcttttt aattctggcc tcggtgacct
     9481 ggctgctttc cagctttgtc gcctggaaac aaacaacgga taacgtcgat gaattgttcg
     9541 acacccaact gatgctgttt gccaagcggt taagtacgct cgatctcaac gaaatcaacg
     9601 cggcggatcg catggcacag acgccaaata aattaaaaca cggtcatgtt gatgacgatg
     9661 cgctgacctt tgccatcttt acccacgacg gcagaatggt ccttaatgat agcgataacg
     9721 gagaagatat tccctatagc tatcaacggg aaggttttgc tgacgggcaa ctggtcggtg
     9781 aagacgatcc ttggcgtttt gtctggatga cctcacctga tggcaaatat cgcatcgttg
     9841 ttggccagga atgggaatac cgtgaagaca tggcgctggc gattgttgcc gggcaattga
     9901 tcccgtggct ggtcgcactg ccgattatgt taatcatcat gatggtacta ctgggtcgtg
     9961 aactcgcgcc gctgaacaaa ctggcgctgg cactacgtat gcgtgaccct gactcggaaa
    10021 aaccactaaa cgcgactggc gtacccagcg aagtgcgacc actggttgag tcgctaaatc
    10081 aactgttcgc ccgcacacat gcgatgatgg ttcgagaacg acgctttacc tccgatgcag
    10141 ctcacgaact acgtagcccg ttaacagcgc tgaaagtgca aaccgaagtt gcgcagctct
    10201 ctgacgatga tccgcaggca cggaaaaaag cactgctcca attacattcc gggatcgatc
    10261 gcgctactcg tctggttgat caactgctca cgctatcgcg gctggactca ctggataacc
    10321 ttcaggacgt cgcggagatc ccgcttgaag atctcctgca atcgtcggtg atggatattt
    10381 accacacggc gcagcaggcg aaaattgacg tgcgactgac actcaatgtc cagggcatca
    10441 aacgcaccgg acaaccgcta ttgctaagtt tgttggtgcg aaatttgctg gataacgccg
    10501 tgcgttacag tccacagggc agcgtggtgg acgtcacgct gaatgccgat aatttcatcg
    10561 tgagggataa cggccccggt gtgacaccag aggcactggc gcgaattggc gaacgcttct
    10621 atcgcccctc cggacaaacc gctaccggca gtggacttgg gctatcgatt gtccagcgaa
    10681 tcgccaagct gcatggcatg aatgttgagt ttgggaatgc ggaacaaggt ggatttgagg
    10741 cgaaagtaag ctggtaaggg taagactttt gctaaattcg caaaagactt tgcacatttt
    10801 gctaatttca ccgtaccgct ctgtgacgta ctatagtcgg caaacgtctc accttgaggt
    10861 taaaaaatga gcaacattct gattatcaac ggcgcgaaaa aattcgccca ctccaatggt
    10921 caactgaatg acaccctgac cgaagtcgcg gatggcacac tgcgcgacct cgggcatgat
    10981 gttcgcatcg ttcgcgccga cagcgactac gatgtcaaag cggaagtaca aaactttctc
    11041 tgggctgatg tggtgatctg gcagatgcca ggctggtgga tgggcgcgcc gtggacagtg
    11101 aaaaaataca ttgatgatgt attcaccgaa ggtcacggga cgctgtatgc cagcgatggc
    11161 cgtacccgca aagatccgtc aaaaaaatac ggttccggcg gcctggtaca gggcaaaaaa
    11221 tatatgcttt ctctgacctg gaacgcacca atggaagcct tcaccgaaaa agatcaattc
    11281 ttccacggcg ttggcgtcga cggtgtgtat ctgccgttcc ataaagcaaa ccaattcctt
    11341 ggcatggaac cgctgccgac atttatcgct aatgacgtga taaaaatgcc tgatgttccc
    11401 cgctatactg aagaatatcg caagcatctt gtggaaattt ttggttaact agagctcagg
    11461 ctttagaagg agttaaccat gcttaccgta atcgcagaaa tccgtactcg tcctggtcaa
    11521 catcaccgtc aggcggtatt ggatcagttt gctaaaatcg ttccaaccgt actgaaagaa
    11581 gaaggttgcc acggctatgc gccaatggtg gattgcgctg ctggcgtgag tttccagtct
    11641 atggcaccgg attctatcgt gatgattgag cagtgggaaa gcatcgcgca ccttgaagcg
    11701 catctgcaaa ccccgcacat gaaggcgtac agcgaagccg taaaaggtga cgtgctggag
    11761 atgaatattc gtattctgca gccagggatt taatcctgcc ttgtttgccc ggccatcctg
    11821 accgggcaat gttctttcct ttaaacctca atctccgcca tgtcgccttt ctcttgcaac
    11881 cagttgcggc gatcttccga gcgtttcttc gccagtagca tgtccatcat cgcgtcagta
    11941 cgctgatcgt cttcatcatc gatagtcaac tgcaccagac ggcgagtgtt cggatcaagc
    12001 gtggtttcgc gcaattgcat cgggttcatt tcccccagac ctttaaaacg ctggacgttc
    12061 ggcttgcctt tcttgcgttt taattgctca agtacgccct ctttctcttc ttccgtcagc
    12121 gcgtaataaa cctctttccc gagatcaata cggtagagcg gtggcagtgc gacgtaaacg
    12181 tgaccgtgtt tcaccaacgc gcggaagtgt tttacaaaca aagcgcagag cagcgtggca
    12241 atgtgcagac catcggagtc agcatccgcc aggatacaga tcttgccgta acgaagctgg
    12301 ctcaaatcgt cgctgtcagg atcgataccg atcgctaccg aaatatcgtg cacttcctgc
    12361 gaagccagca cttcgtcgga agagacttcc caggtgttaa ggatcttacc tttcagtggc
    12421 atgatcgcct gatattcgcg atcgcgcgcc tgcttggcag atccgcctgc ggagtcacct
    12481 tccacaagaa acagttcggt gcggttaagg tcctgcgcgg tacagtcagc cagtttgcca
    12541 ggcagcgccg gaccgctggt cagcttttta cgcaccactt ttttagccgc acgcatacga
    12601 cgctgggcgc tggaaatcgc catctctgcc agcaactccg ccgcctgaac gttctggttc
    12661 agccacagga taaaggcatc tttcaccacg ccagaaacga atgccgcgca ctgacgcgaa
    12721 gagagacgct ctttggtctg cccggcaaac tgcggatcct gcatttttac tgacagcaca
    12781 taggcgcagc gatcccagat atcttccgcc gacagcttta caccgcgcgg cagaatgttg
    12841 cggtattcac agaactcacg catcgcgtcc agcaggccct gacgcagacc attaacatgg
    12901 gtaccgccct gcatcgttgg gatcaggttg acgtagcttt cggtcagcag ttcaccgcct
    12961 tccggcagcc acagtagcgc ccagtccacc gcttcagtat cgccagcgaa attaccgata
    13021 aacggttttt ctggcagcgt cggtaaaccg tttaccgctt ccgccaggta atcattcaga
    13081 ccgtcctgat agcaccagcg ctgttcggtg ttgttgatct catctttaaa ggtaatctca
    13141 acgccggggc acagtactgc tttggctttc agcacatgcg tcaggcgtga aacagaaaaa
    13201 cgcgggctat cgaagaaggt ttcatccggc cagaaatgga cgctggttcc ggtattgcgt
    13261 ttaccgcaag tgccgacaac ctgtaaatcc tgcacctttt cgccattttc aaaggcgatg
    13321 ttataaatct gaccatcgcg gcgcacgtta acttctacgc gcttcgacag ggcgttaacc
    13381 accgaaatcc ccacgccatg caggccgcca gagaactggt aatttttgtt agagaattta
    13441 ccgcctgcat gcagacggca aagaatcagt tcaaccgccg gtacaccctc ttccgggtga
    13501 atatccaccg gcatcccgcg cccatcgtca ataacttcta acgactggtc agcatgtaaa
    13561 ataacgtcca cgcgttttgc gtgacccgcc agtgcttcat ccacactgtt atcaatgact
    13621 tcttgcccca aatggttagg gcgagtggta tcggtataca tccccggacg gcggcgaacc
    13681 ggctcaagcc cggtgagtac ctcaatggca tcagcgttat aagtttgcgt catggtttaa
    13741 gttagtaatt cgagttgatc gtcagagatg gtgcagacca agaaaatcga cgatcgggtt
    13801 gaaataatct tcgaagcccg tgaatgcgtg gttgccgcct tctatgacag tctggcggca
    13861 ggaagcgtag tacgccaccg cctggcggta atccagcact tcatctcccg tctgttgcag
    13921 cagccagatc aaatccggcg cttccagcgg gtcaatctgc atgactttaa gatcgtaaat
    13981 atggcgtgac tctagcacat attgctgccc ggtgtagggg ttctcgttct gaccgagata
    14041 gtccgtcagc agttcaaacg ggcgcaccgc cgggtttacc accactgcgg gcagcataaa
    14101 acattgtgac aaccaggtgg cgtaatatcc ccccagtgac gaaccgacaa tacccagcga
    14161 atcgccgcca tgttccagga caatggattc cagcagctct gccgcgtcgg aaggatacgg
    14221 cggcaactgc ggaatgatca tctcaacgtc agggtgatgt tccgccagcc agttttttaa
    14281 caagctcgct tttgcagagc gcggcgagct gttgaaaccg tgtaaataaa gaagcgtaga
    14341 catcagtagc cttctgaagc ggtatcaggt tggaaacgtg tgtccgccag gcgatgcacc
    14401 tcggtggtca gcgtgccatc agcatgtaac tcgagagtac gccagccggg cgcgatggta
    14461 tccagcgtaa agttggaaca gtgcggctta aactgcacac aggtcgacgg cgttgccagc
    14521 aggcggcgac cgttccagtc gagatccagc tcctgatgaa tatgaccgca cagcaagtat
    14581 ttgacgtgcg gaaacttcgc cagcacggta tccagttcgc ccgcgttacg cagactgtgt
    14641 tgatcgagcc aactacaacc cgcaggtagc ggatgatgat gcagcagcag caacgtatgg
    14701 cgttctggcg catcggccag tttacgttcc agccactcaa gctgaaactc gctcagctca
    14761 ccgtgcggca cgccaaacac ctggctatcc agcaacagga tttgccattg ctcaccaata
    14821 aacacgcgct tcgccgggga gatacccgca tcctgtaacg cgctgtacat cgcgggctgg
    14881 aaatcgtggt tgcccggcag ccagacgcag ggcgcacgaa aacttgcgat gccttcagcg
    14941 aaatgctgat aggccgcaga ggattgatcc tgcgctaaat cacctgtcgc gacaatcagg
    15001 tcgaattcgt gctgatgtgg acgaatcgcc tccagcaccg cctggtagct ttcccaggtg
    15061 tttaccccta acagggcttc gtgcttttgt gcaaacaggt gagtatcggt aatttgtaaa
    15121 atcctgactc tggcctcacc agccagagga agggttaaca ggctttccaa atggtgtcct
    15181 taggtttcac gacgctagta aaccggaatc gccatcgctc catgtgctaa acagtatcgc
    15241 aaccagtccg ctaaaaactg gttaatttga tgcttttcgt cgcgttgatg caacttttta
    15301 ttaggataat cataccgcgc tttgaagcga aaaatctgct ggcttgaaca cacttcagcc
    15361 accatcgcgt catgatacag acgcaccgtc attgacggaa ggctccagta actgatcgcg
    15421 ggcgcagtct gttctattgt caccaaggta gtgtatcggg tcgattccac agtcgtcagc
    15481 cgatattgtg cgtttgccac ctgatagctt acagtttcgc cgggtgcgtc attgcgcggt
    15541 aacaaacggc gcaattgtga aaagttcatc tcgcacaggc gcatcatttc aggaaagtca
    15601 ggtgtgtaac gcttcattta tgcccactca ttttttaacg cttggtggtg cagctgcagc
    15661 cattgcaaag cgatgaccga cgctgcgttg tcgattttcc cctcttctac ccactggtat
    15721 gcctgttccc ggcttaccac atgaacgcga atatcttcgt tttcatcagc cagaccatga
    15781 ataccgcttg cggtcgtggc gtccacttcg cccaccataa ttgacgaacg ctcactggtg
    15841 ccccccgggc ttgccaggaa acttaacacc ggtttggtcc gtttgactat cagccccgcc
    15901 tcttcaatcg cttcgcgacg ggcaacatct tccacacttt caccctcttc aatcatcccg
    15961 gcaaccatct ccagtagcca gggggtttcg ctagtgtcgt acgcggcaat tcgaatctgc
    16021 tcaatcagca caacttcatc acgcactggg tcaaagggta gcaagactgc ggcgtgaccg
    16081 cgctcaaaaa tttcccgccg cacctcatga ctcatttgcc cgttgaatag actatgacga
    16141 aatctataaa gatctaatga aaaaaagccg cgataaagtg tttctcgtgc aataatttct
    16201 acatcgtttt tgccaaatgt aacgggcagg ttgtctggct taagcattgt taatgtcctg
    16261 gcactaatag tgaattaaat gtgaatttca gcgacgtttg actgccgttt gagcagtcat
    16321 gtgttaaatt gaggcacatt aacgccctat ggcacgtaac gccaaccttt tgcggtagcg
    16381 gcttctgcta gaatccgcaa taattttaca gtttgatcgc gctaaatact gcttcaccac
    16441 aaggaatgca aatgaagaaa ttgctcccca ttcttatcgg cctgagcctt tctgggttca
    16501 gttcgttgag ccaggccgag aacctgatgc aagtttatca gcaagcacgc cttagtaacc
    16561 cggaattgcg taagtctgcc gccgatcgtg atgctgcctt tgaaaaaatt aatgaagcgc
    16621 gcagtccatt actgccacag ctaggtttag gtgcagatta cacctatagc aacggctacc
    16681 gcgacgcgaa cggcatcaac tcgaacgcga ccagtgcgtc cctgcagtta actcaatcca
    16741 tttttgatat gtcgaaatgg cgtgcgttaa cgctgcagga aaaagcagca gggattcagg
    16801 acgtcacata tcagaccgat cagcaaacct tgatcctcaa caccgcgacc gcttatttca
    16861 acgtgttgaa tgctattgac gttctttcct atacacaggc acaaaaagaa gcgatctacc
    16921 gtcaattaga tcaaaccacc caacgtttta acgtgggcct ggtagcgatc accgacgtgc
    16981 agaacgcccg cgcgcagtac gataccgtgc tggcgaacga agtgaccgca cgtaataacc
    17041 ttgataacgc ggtagagcag ctgcgccaga tcaccggtaa ctactatccg gaactggcgg
    17101 cgctgaatgt cgaaaacttt aaaaccgaca aaccacagcc ggttaacgcg ctgctgaaag
    17161 aagccgaaaa acgcaacctg tcgctgttac aggcacgctt gagccaggac ctggcgcgcg
    17221 agcaaattcg ccaggcgcag gatggtcact taccgacgct ggatttaacg gcttctaccg
    17281 ggatttctga cacctcttat agcggttcga aaactcgtgg tgccgctggt acccagtatg
    17341 acgacagcaa tatgggccag aacaaagtgg gcctgagctt ctcgctgccg atttatcagg
    17401 gcggaatggt taactcgcag gtgaaacagg cccagtacaa ctttgttggt gccagcgagc
    17461 aactggaaag cgcgcatcgt agcatcgtgc aaaccgtacg ttcctccttc aacaacatta
    17521 atgcatctat cagtagcatt aacgcctaca aacaagccgt agtttccgct caaagctcat
    17581 tagacgcgat ggaagcgggc tactcggtcg gtacgcgtac cattgttgat gtgttggatg
    17641 caaccaccac gctgtacaac gctaagcaag agctggcaaa tgcgcgttat aactacctga
    17701 ttaatcagct gaatattaag tcagccctgg gtacgttgaa cgagcaggat ctgctggcac
    17761 tgaacaatgc gctgagcaaa ccggtttcca ctaatccgga aaacgttgcc ccgcaaacgc
    17821 cggaacagaa tgctattgct gatggttatg cgcctgatag cccggcaccc gtcgttcagc
    17881 aaacatccgc acgcactacc accagtaacg gtcataaccc tttccgtaac tgatgacgac
    17941 gacggggctt cggccccgtc tgaacgtaag acaacgtaaa gatacgggtt atctgccgca
    18001 ttcttccccc ttctcgcttc aatttcgacc agccatcctc tattctgatg ggtatttacc
    18061 actggtcccg gaagacaaaa atgaaacgga caaaatccat acgccacgca tcgttccgca
    18121 aaaactggag cgcacgccat ctgacaccag tcgctctcgc ggttgccact gtttttatgc
    18181 tggctagctg tgaaaagagt gatgaaacag tgtctctcta tcaaaatgct gacgactgtt
    18241 cagctgcaaa cccaggcaaa agcgccgaat gtaccaccgc gtacaacaat gcgctgaaag
    18301 aagccgaacg tactgcgccg aaatacgcca cccgtgaaga ctgtgttgct gaatttggtg
    18361 aaggtcagtg ccagcaggca ccagcccagg ctggcatggc accagaaaac caggcgcagg
    18421 cccagcaatc cagcgggagt ttctggatgc cgctgatggc cggttacatg atggggcgtc
    18481 tgatgggcgg cggcgcggga tttgcacagc agccgctgtt ctcctcgaaa aacccagcca
    18541 gcccggctta cggtaaatat accgacgcga cgggtaaaaa ctatggcgca gcccagccag
    18601 gccgcaccat gaccgtaccg aagacagcca tggcaccaaa accggcgacc actactaccg
    18661 ttacccgtgg cggttttggt gaatctgttg ccaaacaaag cactatgcag cgtagcgcaa
    18721 ccggcacctc ttctcgttca atgggtggct gataccgatg gaaagagtca gtattaccga
    18781 gcgcccggac tggcgtgaaa aagcccacga atacggtttc aattttcaca ccatgtacgg
    18841 cgagccgtac tggtgtgaag atgcttacta caagttgccc ctcgcccagg ttgaaaagct
    18901 ggaagaagtc accgccgaac tgcaccagat gtgcctgaaa gtggtggaaa aagtgatcgc
    18961 cagcgatgag ctgatgacca aattccgcat tccaaaacac acctggagtt ttgtgcgtca
    19021 gtcatggctg actcaccagc catcgcttta ttcgcgtctt gatctggcat gggatggcac
    19081 tggcgaacct aaactgctgg aaaataacgc cgatacacca acgtcactat acgaggcggc
    19141 gttctttcag tggatctggc tggaagatca gcttaacgcc ggtaacttgc cggagggcag
    19201 cgaccagttt aacagtctgc aagaaaagct aatcgatcgc tttgtcgagt tgcgtgaaca
    19261 gtatggcttc cagttgctgc atctcacctg ctgtcgcgac acggtggaag atcgcggaac
    19321 cattcagtat ttgcaggact gcgcgacgga agctgaaatc gctaccgagt tcctctacat
    19381 tgacgatatc gggttaggtg aaaaaggtca gttcacggat ttacaggatc aggtaatttc
    19441 caacctgttc aagctgtatc catgggaatt tatgttgcgt gagatgttct ccaccaagct
    19501 ggaggatgca ggcgtacgct ggctggaacc ggcgtggaag agcattatct ccaacaaagc
    19561 gcttctaccg ctactgtggg agatgttccc gaatcacccg aacctgctgc ccgcttattt
    19621 tgcggaagat gatcatccgc aaatggaaaa atatgtggtt aaaccgatct tctcccgtga
    19681 aggcgcaaac gtgtcgatca ttgagaacgg caaaaccatt gaagcagcgg aaggtccgta
    19741 tggcgaagaa gggatgattg ttcagcaatt ccatccgtta ccgaaattcg gcgacagcta
    19801 tatgctgatt ggtagctggc tggtgaacga tcaacccgcc ggaattggca ttcgtgaaga
    19861 ccgtgcattg atcacccagg atatgtctcg gttttatcca catatttttg ttgagtaatc
    19921 aatgatgccg gatggcattc gccatccggt aattgttaac ctatctgcac cgacagcata
    19981 ctcaggctgc ccatttctat accatcgacc ggaatggtaa ttggctcctg cccatcccac
    20041 gcacctaaga catacaacaa cggcagatag tgctccggcg ttgggttcga taacgcgcca
    20101 ccttcatggt cgaggtaatt caccagagga tgctgctcta ccgccccttg ccacgtcaga
    20161 ttcgctttca catactcatt aaacgacatc gcccaaggat atggtgaact atcaccgtgc
    20221 cacttcactg tacgcaggtt atgcaccacg ttgccactgg cgaccaacat tattccttca
    20281 tctcgcagcg ctgccagttt gcgccccatt tcgaaatgcc aggcggcagg tttgctactg
    20341 tcgatactca actgcaccat cgggatatcg gcgtcaggat acatcttaat cagcacgccc
    20401 caggaaccgt ggtcaaagcc cctggcttct ttatccagcg tcaccgggac cggcgctaac
    20461 agctcaacca gatgctgtgc cagcacaggc gaacccggag caggataatg cgtatcgtac
    20521 agcgcctgcg ggaagccacc aaagtcatga atcgtgggcg gcgtctccat cgcggtcact
    20581 cctgttccac gggtaaacca gtgagccgaa accaccacaa tcgcttgcgg gcgtggcaat
    20641 gtcatcccca acttctgcca gctgcgggta tacaaattat cttccagcac gttcatcgga
    20701 ctaccgtgac ctaaaaacaa tgctggcata cgtgttgaag acatgatgat atccttaact
    20761 aagggtgtca ttttgatatc ctcacaatac gcttgttcgg cggagtaaga acccggataa
    20821 caatgatgat gatcatcagt tattttgacg atctgcctga aggtgaagat ttataaggag
    20881 ttgtcgatgt cagtacctct cattctgacc atactggcgg gggcagccac gtttattggc
    20941 gcgtttctcg gcgttctcgg acaaaaaccc tcgaaccgct tactggcgtt ttcgctaggt
    21001 tttgcggcgg ggatcatgtt gctcatctca ttaatggaaa tgcttcctgc cgcactagca
    21061 gctgaaggaa tatcgcctgt gttgggttat ggaatgttta tcttcggtct gcttggctac
    21121 tttggcctgg accgcatgtt gccgcatgcg catccgcagg atttaatgca aaaatcggtg
    21181 cagccgttgc caaaatcgat caagcgcaca gccattctgc tcactctcgg catcagtctg
    21241 cataacttcc cggaagggat tgccaccttt gtcacggcga gcagcaacct ggagctggga
    21301 tttggcatcg cactggccgt cgcgttgcac aatatccctg aaggtctggc agtggcaggc
    21361 ccggtttatg cggcaacggg gtctaaacgc accgcgattc tgtgggcggg gatttctgga
    21421 ctagcagaaa ttcttggtgg tgtgctggcg tggttaatcc tcggtagcat gatttccccg
    21481 gtggtcatgg cggcggttgc aggaattatg gtggcgctct cggttgatga attaatgccg
    21541 ctcgccaaag agattgaccc taataataac cccagctatg gcgtactatg tggaatgtca
    21601 gtgatgggat tcagtttagt gctgctacaa acggcgggaa ttggttaaaa acaacatcag
    21661 aagttgtcca gcacatggac aacttctgcc agcttcacga aagccttatt ctaccttttc
    21721 ggctgtctct tctctcgtac tgtttaagta tttgttccgc gcttcaagca gtgcgtctct
    21781 gtcctggcga aacgcccgac aataacgttt caaatgcagg aaataaccca ccacaaccag
    21841 agcaatcaat acaatccagt accaggcgat aaacatcctt tacccttaat atttaacaat
    21901 actaaacatt tagcgcataa atttcacata tcctttttcg gatatattca tatggtcgca
    21961 taataacgga acaattatat gattttgatc ataatgaaaa agaatgcctg atattaatga
    22021 aattttctca tagaaagaga agcggaatta acatcttgca tagcagggca aaataaaaac
    22081 cgaagccagc tacaacttcg gtttcttgat taaggcagta attaagcagc ggttttcagc
    22141 tggctttacg ctcatgtgcc tgacggtatg ccaccaggtc ttcaatagtc acgagcgcca
    22201 tattgtgttt attggcaaac tcaatgcact ctggtgcacg cgccatcgtg ccatcgtcat
    22261 tagtcagctc acacagcaca ccagccggtt taaagcctgc cagcgtcatc aaatcaatag
    22321 tcgcttcagt atgaccgcca cgcgtcagta caccacctgc ctgcgcgcga agtgggaaaa
    22381 cgtggcccgg gcgattcaga tcagaaggtt ttgcgccatc ggcaatcgct gcgcgaacgg
    22441 tcgtaatacg atcagcggca gaaacaccgg tagtcacacc ttcagccgct tcaatggtca
    22501 cggtaaaacc ggtgccatag gcgctggtgt tattttctac catcattggc agatcaagtt
    22561 gtttacggcg atcttcagtg atgcacaggc aaacaatacc gctaccgtgg cgaatggtca
    22621 gcgccatctg ctcaacggtc atggtttctg ccgggaagat catatcgcct tcgttttcac
    22681 ggtcttcatc atcaagcacc attacaccgc gtccttcacg cagcgcagcc agtgcatttt
    22741 caacacgttc gaaaggcgta ccaaaagagg aaagtagcgt ctgattcatg gtaaaaaaaa
    22801 ctcactaaaa ttatggttac cagaatcagg gcagtcttag gagtggcggc atatagccaa
    22861 aataacgtga gcgggtccat gcccgacaga atcgttactc tctcccatcc ggactctaac
    22921 cgtcggcccc ggaattacac cggatctgct gtcctttgag ttcgcaccca aagcgctcgc
    22981 gggctttcaa ctgagttgat ttaccgccgg tggggaattt cgccccgccc tgagaataag
    23041 cggattcact ataacgctaa tgattagcgg cagcaacgca tagcttcaca taattctggt
    23101 ttatgactta cccttatcgc actacaatgg tactcaacac ctatcagtac agggaaacta
    23161 caatgattga cccgaaaaaa attgagcaaa tcgctcgcca agttcacgaa tcaatgccta
    23221 aaggaatcag ggagttcggg gaagatgtgg agaaaaaaat ccgccaaacc ctacaagctc
    23281 agctgacgcg cctcgacctt gtaagccgtg aagagttcga cgtccaaacg caagtcctgt
    23341 tacggactcg tgagaaactg gcgctgcttg aacaaagaat gagcgaactg gaaaatcgca
    23401 gtactgagat taaaaagcag ccagatccag agactctccc cccaacgctg taaacatttg
    23461 tctttaatgc actgtaaatt atgaattaat cttctaattt acagtgcttt ctattacctt
    23521 a
//
LOCUS       DAZPEB010000043        23494 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZPEB010000043 DAZPEB010000000
VERSION     DAZPEB010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23494
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.044"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(299..613)
                     /gene="pspE"
                     /locus_tag="ACHURJ_001525"
     CDS             complement(299..613)
                     /gene="pspE"
                     /locus_tag="ACHURJ_001525"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFR1280528.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(688..909)
                     /gene="pspD"
                     /locus_tag="ACHURJ_001526"
     CDS             complement(688..909)
                     /gene="pspD"
                     /locus_tag="ACHURJ_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFR1280529.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(918..1277)
                     /gene="pspC"
                     /locus_tag="ACHURJ_001527"
     CDS             complement(918..1277)
                     /gene="pspC"
                     /locus_tag="ACHURJ_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFR1280530.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(1277..1501)
                     /gene="pspB"
                     /locus_tag="ACHURJ_001528"
     CDS             complement(1277..1501)
                     /gene="pspB"
                     /locus_tag="ACHURJ_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFR1280531.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(1555..2223)
                     /gene="pspA"
                     /locus_tag="ACHURJ_001529"
     CDS             complement(1555..2223)
                     /gene="pspA"
                     /locus_tag="ACHURJ_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFR1280532.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKESGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            2390..3367
                     /gene="pspF"
                     /locus_tag="ACHURJ_001530"
     CDS             2390..3367
                     /gene="pspF"
                     /locus_tag="ACHURJ_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFR1280533.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPATVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(3487..4752)
                     /gene="puuE"
                     /locus_tag="ACHURJ_001531"
     CDS             complement(3487..4752)
                     /gene="puuE"
                     /locus_tag="ACHURJ_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HFR1280534.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(4790..6059)
                     /gene="puuB"
                     /locus_tag="ACHURJ_001532"
                     /pseudo
     CDS             complement(4790..6059)
                     /gene="puuB"
                     /locus_tag="ACHURJ_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
     gene            complement(6061..7548)
                     /gene="puuC"
                     /locus_tag="ACHURJ_001533"
     CDS             complement(6061..7548)
                     /gene="puuC"
                     /locus_tag="ACHURJ_001533"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFR1280535.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLVGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FSDCSDLQKAASSTAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEEQALQLANDSQYGLGAAVWMRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(7823..8380)
                     /gene="puuR"
                     /locus_tag="ACHURJ_001534"
     CDS             complement(7823..8380)
                     /gene="puuR"
                     /locus_tag="ACHURJ_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFR1280536.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(8560..9170)
                     /gene="puuD"
                     /locus_tag="ACHURJ_001535"
                     /pseudo
     CDS             complement(8560..9170)
                     /gene="puuD"
                     /locus_tag="ACHURJ_001535"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
     gene            9382..10800
                     /gene="puuA"
                     /locus_tag="ACHURJ_001536"
     CDS             9382..10800
                     /gene="puuA"
                     /locus_tag="ACHURJ_001536"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFR1280537.1"
                     /translation="METNIVEVENFVQQPEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDNRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKLLVRLMAEKHKMHSTFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLALNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            10949..11114
                     /locus_tag="ACHURJ_001537"
                     /pseudo
     CDS             10949..11114
                     /locus_tag="ACHURJ_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11104..12489
                     /gene="puuP"
                     /locus_tag="ACHURJ_001538"
     CDS             11104..12489
                     /gene="puuP"
                     /locus_tag="ACHURJ_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFR1280538.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSINLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YDGDRTPVSET"
     gene            12623..12868
                     /locus_tag="ACHURJ_001539"
     CDS             12623..12868
                     /locus_tag="ACHURJ_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2543 family protein"
                     /protein_id="HFR1280539.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            13181..14824
                     /gene="sapA"
                     /locus_tag="ACHURJ_001540"
     CDS             13181..14824
                     /gene="sapA"
                     /locus_tag="ACHURJ_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFR1280540.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            14821..15786
                     /gene="sapB"
                     /locus_tag="ACHURJ_001541"
     CDS             14821..15786
                     /gene="sapB"
                     /locus_tag="ACHURJ_001541"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFR1280541.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            15773..16663
                     /gene="sapC"
                     /locus_tag="ACHURJ_001542"
     CDS             15773..16663
                     /gene="sapC"
                     /locus_tag="ACHURJ_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFR1280542.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            16663..17655
                     /gene="sapD"
                     /locus_tag="ACHURJ_001543"
     CDS             16663..17655
                     /gene="sapD"
                     /locus_tag="ACHURJ_001543"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFR1280543.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVIADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            17657..18463
                     /gene="sapF"
                     /locus_tag="ACHURJ_001544"
     CDS             17657..18463
                     /gene="sapF"
                     /locus_tag="ACHURJ_001544"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFR1280544.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            18531..18884
                     /locus_tag="ACHURJ_001545"
     CDS             18531..18884
                     /locus_tag="ACHURJ_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFR1280545.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            19252..20040
                     /gene="fabI"
                     /locus_tag="ACHURJ_001546"
     CDS             19252..20040
                     /gene="fabI"
                     /locus_tag="ACHURJ_001546"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFR1280546.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            20185..21312
                     /locus_tag="ACHURJ_001547"
     CDS             20185..21312
                     /locus_tag="ACHURJ_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HFR1280547.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTARDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEYTKSSTAELCQLAETLSLHPISLSLLERLLNSTRVNTQPDNKLAALLCARIN
                     GSPACFAACMDSSNEYKKISPLLRKGENEINQWADRHSVEHATVQAIQWLTRAPDRFS
                     TAQFSPLLEHEQSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            21380..23314
                     /gene="rnb"
                     /locus_tag="ACHURJ_001548"
     CDS             21380..23314
                     /gene="rnb"
                     /locus_tag="ACHURJ_001548"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFR1280548.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGAIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAMRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(23392..23494)
                     /locus_tag="ACHURJ_001549"
                     /pseudo
     CDS             complement(23392..23494)
                     /locus_tag="ACHURJ_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         5308 a         6207 c         6118 g         5861 t
ORIGIN      
        1 aagacgcgtt acaagtttct gtaaatgagt tgcggtaaat gttccaccgt agatttcgtc
       61 caggtaatca gtaattttct gtttcatgtt gcctttcagt ggtagcgatc ccatgaaacg
      121 agagactact ccgcagaaaa taaactgtca attgagcaca agggttttag tcacgcagga
      181 aatcattttt taccagggaa aaagcgtatt tgtgagctga cgcaaataat ctatggggat
      241 gtaaataagg tgtgacagaa aaaaaacggc gcataagcgc cgctcatggt gaattctttt
      301 aacctttgac cttcggcatt gcgatgtctt tcaagccacc ggcgttctca acgtgggtat
      361 atcccatctc gctaaggatc tcttttgctt gccctgactg gcgtccggca ttgcaataca
      421 ctttcacggt gtcgttttta tccggaacgg cggtggcaat gcgctctttc acttccttca
      481 ggggaatatt gatggccccc tgaacgtgct cttgctgata ctgctctgga acacgaacat
      541 cgatccagtg ttcagcggcg aaaacgggca gtgaaaacac cagtgccaga gcaagtaagc
      601 cttttttaaa catgtaagcg tccttctatg gatgaaaatt gccatccatt atacgcctga
      661 gatttaacaa tttttgcgaa actcccctca ccttttataa cgctgtgcca gtttattagc
      721 agcccgactt aacagcggtt ccagcgccac agccagcaac attttcagcg gtcggcgagc
      781 aactgatttt atcgcccagc ccgctacacc cgccgggcca tagcgcagtg cggtaagaag
      841 taccagcttg cctgctaatt tgaaaccagg ctttaccttt tgcccggcct gttgccagcg
      901 agtattcata actttcctca cagttgacgg aaacggctac gtaacgtgaa agtatcggaa
      961 gtgacataac gttccatctc gcgtaaacgc gtttcacttg ccgccagttc acggtcgact
     1021 tcatccagca attcgctgct ggagggtagc tgctcaccaa aggccatgtt gtccggcatt
     1081 ggatcaagcg caaatgacaa aatgatgtaa gcaaccaggg taaacagcgc cagaccgaag
     1141 aaaatcgaca gcaccaccag gatacgcacc agttttaccg gtacatcaaa atagttggca
     1201 atcccggcgc agacgccgcg gaccatgccc tgctgtggaa tacgccataa ttttttattg
     1261 agattaatgc ccgccattag cgatccctcc agttcggatg ttctgcatca agaatagatt
     1321 ccagcgcctg aatacgttcg cgcatccgtt ttgcttcatc agccagttgc gctaatcgct
     1381 gctgctcact ttgcgacaat tcactgcgac cagaacgatt gctgtaatgc agccataacc
     1441 agatcggtaa aacaaacagc acaaaaatgg ttaacggaat agccagaaat agcgcgctca
     1501 taagtactcc ttagacgatg agcggcggcg cgttcggacg ccgcctagat gttattattg
     1561 attgtcttgc ttcattttgg cttttaattg tgccagttgt tcgctgattg catcatcggc
     1621 tttcagttcg gcaaactgat cgtccagcga tttttgttta ccgaagctgt ggctttctgc
     1681 ttccgcttcc atctggtcaa tacgacgttc gaaagattcg aaacgagcca ttgcttcatc
     1741 cagtttgcca ctgtccagct gacgacgcac atcgcgcgac gagtttgccg cctgatggcg
     1801 taacatcaat gcctgctggc gagcgcgtgt ttcgctcaat ttgttttcca gctcaccgct
     1861 ctctttcttc atgcgtgcca gcgtatcgtc caccagcgtc acttcatgtt ccagactctt
     1921 aatcagatcg gtcagtttct gtttttcaat taacgccgca cgtgccaaat cctctctctc
     1981 tttcagcagc gccagttcgg ctttttcctg ccattcaact tcacgcgccg acgcttgttc
     2041 aatacggcga gtcagctgtt tcttttctgc caacgcacgc gccgaagtag aacgtacttc
     2101 aaccagtgta tcttccatct cctggatcat cagacgcacc agtttctgtg gatcttccgc
     2161 tttctctaac agagcgttga tgttggcatt cacgatgtcg gcaaagcgag aaaaaatacc
     2221 cataatgttg tcctcttgat ttctgcgttc aggattgtcc tgctgaactg ttaatacaat
     2281 ttgcgtgcca attttttatc tttttgattt ataaagatct gattgaagaa tcatcagcaa
     2341 catgccagga tgaattagcg aattacacta acaagtggcg aatttcatca tggcagaata
     2401 caaagataat ttacttggtg aggcgaacag ctttctcgaa gtgctggaac aggtttcgca
     2461 tctcgcaccg ctggacaaac cggtgctcat catcggcgaa cgcggcaccg gtaaagagct
     2521 gattgccagc cgcctgcatt atctctcctc ccgttggcaa gggccgttta tttcccttaa
     2581 ctgcgcggcg ttaaatgaaa atctgctgga ttccgaactg tttggtcacg aagcgggggc
     2641 gtttaccggt gcgcaaaaac gtcatccagg gagatttgaa cgtgccgacg gcggtacgct
     2701 atttcttgat gaactcgcta cggcaccgat gatggtgcag gagaaattat tgcgcgtgat
     2761 tgagtacggt gaactggagc gcgttggcgg cagccagcca ttgcaggtga atgtgcggtt
     2821 ggtatgcgcg acgaatgccg atctcccggc gacggtcaat gaaggcactt ttcgcgctga
     2881 cctgctcgac cgactggctt ttgatgttgt acaactgcca ccactgcgcg agcgcgaaag
     2941 cgacataatg ttgatggcag aacactttgc catccagatg tgtcgggaaa tcaagctgcc
     3001 tctgttcccg gggtttacgg agcgcgccag agaaacattg ctgaattatc gttggccggg
     3061 aaatattcgt gaattgaaaa acgtggtgga acgttcagtg tatcgccacg gcaccagcga
     3121 ttatccgctt gatgacatca ttattgatcc ctttaaacgg cgtccgcctg aagacgctat
     3181 cgccgtttca gaaaccacct cgcttccaac actgccgctg gatttacgtg agtttcagat
     3241 gcagcaggaa aaagagttgc tgcaactcag tttgcaacaa gggaaatata accagaagcg
     3301 cgcggctgaa ttactggggt taacttatca tcagttccgc gcgttgttga aaaagcacca
     3361 gatttagcga agttgagcaa tacaacttgc cggatgcggc gtgaatgctt tattcggcat
     3421 acataatcaa taagtcgttt gcaggcctga taagcgcagc gcatcagaca ttattgcgtt
     3481 gggctattaa tcgctcagcg catcctgcaa aattttcatt gccgcatcga attgcgcatc
     3541 cggaatggtc agcggataca ggaagcgaat cacgttgccg tatgcgccac aggtcagcag
     3601 gagcagcccc tgcgccagcg cgcgttgctg gattttctgt gcaatcgccg ctgacggctc
     3661 gcccgtttgc ggatcgttaa actctgccgc aatcatcgac cccaggccgc gtaccgcagc
     3721 aatggccgga acgctttctt tggcatcaat caacgtgttt ttgagacgct ggcccagttg
     3781 attcgcgcgt tcgcagagtg attctttgtc gataatattg agcaccgcgt gcgcggcagc
     3841 caccgccagc gggttcccgg cgtaggtgcc acccaacccg cccggcgcgg gcgcgtccat
     3901 aatattcgcg ttaccgacca cgcccgaaag cggcatcccg cccgcgaggc ttttcgccat
     3961 cgtcattaag tccggcttat cggcgtaatg atccatggca aacagtttac cggtacgcgc
     4021 aaagccgctt tgcacttcat cggcaatcat cacaataccg tgctcgtcgc acaggcggcg
     4081 aatggcggca accagctctt ttggcgcaac gttgaaaccg ccctcgccct gcaccggttc
     4141 gaaaataatg gccgccacct gcttcgcttc aatgtccgat ttaaacaagc gttcgatggc
     4201 gtcgagggag tcctgtgttg aaatgccgtg taaatctgac ggataaggta cgtgatacac
     4261 cgaaccaggg aacgggccga agccgatttt gtacggcgca acttttccgg tcagcgccat
     4321 ggtcatatat gtacgaccgt ggaagccgcc gctaaacgca atcacgccag gtcgtccggt
     4381 atgggcgcga gcaattttca ccgcgttttc caccgcttcc gcgccggtag tgaagaatgc
     4441 ggttttagcc tgcccgctca ctggggcaag ggcgttaatt ttctccgcca gcgtgacgta
     4501 gctttcgtac ggcacaatct gatacgccgt gtgggtaaac tgttgcaatt gctgctccac
     4561 cgccgcgacc agatcagggt ggcgatgccc ggtattcagc accgcaatgc ctgcggcgaa
     4621 atcgatgtat tcgttgccct ctacgtcctt cagcgtagcg ttttctgccg actgggcgaa
     4681 gaagttacac atcacgccaa ccccgcgtgg ggtagcagaa agacgacgct gatggaattc
     4741 attgttgctc atgtttcaca ccttttgact cttcactgtt aatcggaaat taaacgccca
     4801 gacgatcgcg caggctgtaa taagccgcgc ccatcgcggt aaatggcacg cgcagcgtgc
     4861 gcccgccggg gaacgggtaa tgcggcagat tggcgaaggc atcgaaacgc tcggcgtcac
     4921 cgcgcagcag ttcagcaatc aaacgcccgg cgaggtgggt acaggtgacg ccgtggccgc
     4981 tgtagccctg catgtaatag atgttggtat cgaggcgacc gaactgcggc atccgtgaaa
     5041 gggtcagcag gaagttgccc gtccagcgat agtcgatttt gacgcccttc agctgcggga
     5101 aggttttcag cagtttcggc acaacaagac gctcaacgtc atccgggtcg cgcgcgccgt
     5161 agaccacgcc gccgccgtac agcaaacggt tgtcggcggt aagacggtag taatcaagca
     5221 gataattaca gtcttccaca cagtagtttt tcgggatcag cgaacgggct aaatcttccg
     5281 acagccgttc ggtggtgatc acctgcgtgc cgcacggcat gctgcgtttc gccagttccg
     5341 gctctacttt atcgcccaga tacgcattcc cggcgacaat cacatacttc gccgtcacct
     5401 gacctttggc agtgcgcaca acggctggcg tggtgtgctg gatctgcgtc acggcagaaa
     5461 gttcatacac gcgcccgccg ttgaggcgga tggcgtccgc ttcgccaatg gcgaggttta
     5521 gtgggtgaat atgcccaccg ctgtgatcca gcagcgcacc ggtatagcga tcgctggcta
     5581 cttcacggcg aatggcgttg gcgtccagca attccagctg tttattgccg tagcgttccc
     5641 agttctcttt ctgctcttca agtgtggcga gctgtttatc attcatcgcc acaaacaggc
     5701 tgccggggcg gtagtcgcag tcaatctgat aacgtttgat acgttcgcgg atgatctcac
     5761 caccttcgaa catcatgctg ccgagcatag gggcggtgtc catgccgtag cttttttcga
     5821 tcacgtcgat gtcgcggttg taggagttca caagctgccc gccattgcgc ccgcttgcgc
     5881 caaagccgat gcgtgaggct tcgagaacca ctacgtcaaa gcccgcttcc gccagatgca
     5941 gcgcggagga gagcccggta tagccgccgc caaccacgca aacgtcgcag gtgattgact
     6001 cattcagggt gtcgaatggc acgtatttat gcgtaataac tgctggtatg ttcggtcatt
     6061 tcaggcctcc aggcttatcc agatggtttt cagttcggtg aatttttcaa gggcatgcag
     6121 ggatttgtcg cgaccgttgc cgctctgctt atagccgcca aacggcacgg tcatatcgcc
     6181 gtcgttgtag ttattgacga agacggaacc ggctttcagg cggcggctca tacggtgcgc
     6241 gcgggagagg tcgcgcatcc ataccgccgc gccaaggccg tactggctgt cgttggcaag
     6301 ctgtagcgcc tgttcttctg atgtgaaacg cgtgaccacc agcaccggac cgaaaatctc
     6361 ttcgcggctt aaggacgcat tcgggtctac atccacaaag atggtcgggc cgatggcggc
     6421 agccagcccg gcgttacggc catccagcag cagttgccct ttgccttcgc cctcacgaat
     6481 aaagctatgg accgagtcgg cgtgggcgca gtcgattaaa gtgcccatgg tggttgcggg
     6541 gtcaagcgga tggcccggct gccagttttg cgcctgctgt tttaacaggg ctaagaattc
     6601 atcggcgatg ctctcttcca gcaacaggcg tgttccggcg atgcacacct gaccctggtt
     6661 gtagaagatg cctgctgcgg tggagctcgc cgctttttgc agatccgaac aatcggagaa
     6721 aacgatgttg gcgcttttgc cgcccgcttc cagccagacg cgtttcatgt tgctgtcgcc
     6781 cgcatctttc agcagctgtt tcccggtacg ggttgaaccg gtaaaggcaa tggcgtcgat
     6841 atcgttatga cgcgacagcg cctgccccgc ttcatgacca aaacccgtca ccacgttcaa
     6901 cacaccatcc ggcaagcctg cttctttcgc cagccccacg agacgaatcg cactgagcgg
     6961 tgatttttca gacggtttta gaatcacgct gtttcccgcc gccagcgccg ggccgagttt
     7021 ccagcaagtc agcaacagcg ggaagttcca cggcacgatg gcggcaatca cgccgaccgg
     7081 ttcacgcacg atcatcgcca gctcatggct actggtggtc gccacttcgc catacacttt
     7141 gtcgatcgct tcggcgtacc agcgaatggc gcgcgccgcg ccgggaatat catcacgcag
     7201 actgtgacga atcggtttgc cggtgtcgag agtttccagc agtgccagct cttcggcgtg
     7261 ggcttccatt aaatcggcga gtttattcag taccgcttta cgtttagccg gagaagagag
     7321 tgaccagtcg ccgcgttcaa atacgccgcg tgctgcgctc accgcacggt cgatatcgac
     7381 gctcttgccg cgggcaattt tcgccagcgg tgcctgggtg actggatcaa cggtttcaaa
     7441 ggtttcattt tccgccgcag cagtatattc accgttaata aataagcggt tttcaatggc
     7501 gagacttaac gctttatcct gccagtaagc cagatgatga aaattcatta tgactcctgt
     7561 ttcacgtcta tcagatatat gcagtttgtg aacgtcaggg cgcagagatc cccgggccgt
     7621 atgcccgttt atttctggcg acattcttta tatacacttc atccttcaag ccgcctctgc
     7681 cttggctgcg ttcgctcccc cgaatcactt acctgagtaa gctcatcggg attcgctcac
     7741 ttgccgcctt gatgcaactt gaatgatttt gtgtatagat tgccgggcgc attgcgctta
     7801 cccggcttac agaacaaaaa gattaaaacg tggtgggcgt atgggcgctg ataattcggc
     7861 aaatacctgc cgacgtatta ctgaaactgt gcgggatgcc ggtattaatg gcataacttt
     7921 gacccgcgac gaggtggtaa tcctgaccat taatcgtcag aacaatttca ccttccagta
     7981 ctgtgcctat ttcctcaccc tgatgcttaa ttctttcccc agtggttgtg cccggctggt
     8041 acgtttcaaa gatcatcgcc agcgtgcgat tcgggttacc gttatgaacc agcttcattg
     8101 acacaccctg actgcccatc tcaattaagt cgtcctgatt aatgacgacc tgcggctcat
     8161 caggtttttc cggctcggaa aagaattccg agagtgacag accatacacc ttcagcagct
     8221 tttgcagcgt actgatggca gggctgactt tatcttgttc tatcgtactg atagcactgt
     8281 gagtcagccc ggagagttcg gcggcacgac gttgtgaaag cccctgctgc tggcggattt
     8341 ccgacaagcg ttttcctggc gccagtccct catcactcat atttgcattt ccttaaactg
     8401 tagtggtcag agtcgctgtt tttcagcgat atggtgctga caagcggtga taaagccctc
     8461 gaacaatata cgcgaaagag cgtactcgct actgttccat tccgggtgcc actgtacgcc
     8521 cagcgcaaaa ggatgattga tgacgctaac cgcctcaact aagccatccg gcgagcgagc
     8581 ttcaacacgc aaccgtgggc taacgacctt cgccccttgt ccatgtagag agtttaccca
     8641 aaagttgcta cattcaggta acaacgcaga cagtaatccc ccctcttcaa cctgaacttc
     8701 atgcgacggt gcatattgct gttccaccgg cagttcggat cttcccgatg ttccagcaat
     8761 tcaggctgtt cgcacagctt gcgatgcaac gaaccaccgg ttgccaccac cagttcttgt
     8821 aaaccccggc agatggcgaa aatggggatg cgcctttcga gtgcggcatt aattatcgcc
     8881 atgctcagaa gatcacgccc gggatcggcg tcaggctcat cgccgttttc accatatagg
     8941 tgcggctgca cattgctggg actaccagga agataaatgc catcgagttt cggcaaaagt
     9001 tgttcaagta acgacggttc cgccagcgca tgtggtagcg caattggcaa gccgcctgcg
     9061 tggatgatgg cattcaggta cttttcttgc agagtctggg tcgcatgacc cttaagcctg
     9121 ttcctgcaca ttacgacacc gataaccgga ttgttcatta tatttttcat gctcaatctc
     9181 acaaagtgga ctaaattatc gccattactg cgctttaaat gacctttatg ttcaatattt
     9241 tttcaatcta gcagtgggtt ttcatttttg caaactaaat ttaacatttg gcaaacattt
     9301 agtttgcata cagattcgaa tggtggtcat tatattttac gctttgataa cgagcggaaa
     9361 acaaaccaaa ggcgaagaat catggaaacc aatatcgttg aagtagagaa ctttgttcag
     9421 cagccagaag agaggcgggg tagcgccttt acgcaggaag tgaaacgcta cctggagcgc
     9481 tacccgaata cgcaatatgt tgatgtttta cttactgatt tgaatggttg cttccgtgga
     9541 aaacgtatcc ctgtttcaag cctgaagaag ttggagaaag ggtgttattt cccggcctcg
     9601 gtgtttgcaa tggatattct gggcaacgta gtagaagaag cgggtctggg tcaggaaatg
     9661 ggcgagccgg atcgtacttg tgttcctgtt ctcggttcct taaccccttc tgccgccgat
     9721 ccagagttta tcggtcagat gctcctgacc atggtcgatg aagatggcgc tccctttgac
     9781 gttgagccgc ggaacgttct taaccgcctg tggcagcagc tacgccagcg cggattgttc
     9841 ccggttgtag cggtagagct ggagttctat ttactggatc gtcagcgcga cgctgaaggg
     9901 tatctgcaac cgccctgcgc gcccggcacc gataaccgca atacacaaag ccaggtttac
     9961 tccgttgata acctcaacca cttcgccgac gtgctcaatg atattgatga actggcgcag
    10021 ttacagttaa ttcccgccga cggtgcggtc gccgaagcct cgccgggtca gtttgaaatc
    10081 aacctttacc ataccgataa cgtgctggaa gcctgcgatg atgcgctggc gctaaaactc
    10141 ctggtgcgtc tgatggcaga aaagcataag atgcactcca cttttatggc gaagccgtat
    10201 gaagagcacg cgggcagcgg gatgcatatc catatcagta tgcaaaataa tcgtggcgag
    10261 aatgtgcttt ctgacacaga aggcgaagat tcgccgctgc tgaaaaagat gctcgccggg
    10321 atgattgacc tgatgccgtc gtcgatggcg ttgctggcac taaacgtgaa ctcgtatcgc
    10381 cgcttccagc cgggaatgta tgtgccgacg caggcgtcgt ggggccataa caaccgcacc
    10441 gtcgccctgc gtattccgtg cggcgaccgt cataatcacc gcgtggaata tcgcgtggcg
    10501 ggtgccgatg ccaacccata tctggtgatg gcagcgattt ttgccggtat tttgcatggc
    10561 cttgataacg agctgccgtt gcaggaagaa gtcgaaggca acgggctgga acaggaaggc
    10621 ttacccttcc cgattcgcca gagcgatgcc ctgggtgagt ttatcgagaa tgatcacctg
    10681 cgccgctatt taggcgaacg gttttgccat gtgtatcacg cctgtaaaaa tgatgagctg
    10741 ttgcagtttg agcgcctcat cacagaaaca gaaattgagt ggatgttgaa aaacgcatag
    10801 taccccttgc ggggttgttt catcgacctg agcggagcca ggcctgacat ttgctggtaa
    10861 tccagacatt tcccggatgt cgcgttatgg agcgcaggcg gcaacgggct tccgttaacg
    10921 attaagactg tgacgaggaa atgagatgat gccgatgatt aaatccccgc atggcgaggg
    10981 aggctgcgta tgcgcccctc ctgctactga ctggactccg tcccctttgc ttcccttgct
    11041 aaacaggttt gatttcaggt cgacacgacc gcaaacctta ttacgcaggg ggaggcagca
    11101 attatggcta ttaattcacc actgaatatt gctgcgcaac ccggcaaaac ccgtctgcga
    11161 aaatcactga aattgtggca ggtggtgatg atgggtctgg cctatctcac gccgatgacc
    11221 gtatttgata cttttggtat tgtctccggc attagcgacg gtcacgttcc ggcgtcctat
    11281 ttgctggcgc tggcgggcgt gctgtttacc gctatcagct acggcaaact ggttcgccag
    11341 tttccggagg ccggttcggc ctatacctac gcgcaaaagt cgattaaccc gcacgtcgga
    11401 tttatggtcg gctggtcatc gctgctggat tatctctttt tgccgatgat caacgtcttg
    11461 ttggcgaaaa tctatctctc cgccctcttc ccggaagtgc cgccgtgggt gtgggtagta
    11521 accttcgtcg ccattttaac cgccgcgaat ctgaagagca tcaacctggt cgctaacttc
    11581 aataccctgt ttgtactggt gcaaatctcc atcatggtgg tgtttatctt cctggtagtt
    11641 cagggactgc ataaaggaga aggcgttggc accgtctggt cacttcagcc gtttatcagc
    11701 gagaacgcgc acctgatccc gattattacc ggggcgacga ttgtctgttt ctccttcctc
    11761 ggtttcgatg cggtgaccac gctttcggaa gagacgccgg acgccgcacg cgtgatcccg
    11821 aaagccatct tcctgacggc ggtctacggt ggcgttatct ttatcgcggc gtcgttcttt
    11881 atgcagctgt tctttcccga tatcagccgc tttaaagatc cggatgccgc actgcctgaa
    11941 attgcgctgt acgtcggcgg caagctcttc cagtcgattt tcctctgcac cacgtttgtg
    12001 aacacgttag cctctggcct ggcttcacat gccagcgtgt cgcgtctgct gtatgtgatg
    12061 gggcgtgaca atgtgtttcc ggagcgcgtg tttggctatg tgcacccaaa atggcggact
    12121 ccggcactca acgtcattat ggtcgggatt gtcgcgttgt cggcgctgtt cttcgattta
    12181 gttaccgcca cagcgttaat taacttcggt gctctggtgg cgtttacctt cgtgaatctg
    12241 tcggtgttta atcatttctg gcggcgtaaa ggaatgaata aaagctggaa ggatcacttc
    12301 cactatttgc tgatgccgct ggttggcgcg ctgacggtgg gtgtgctgtg ggttaacctc
    12361 gagtcaacgt cactgacact cggtctggta tgggcttcgt taggcggcgc atatttatgg
    12421 tatctgatcc gccgctatcg caaagtgccg ttgtatgatg gtgacagaac gccggtgagt
    12481 gaaacgtaat agtcggatgc gcggcgcatt atcctgccgg agggtaatgc accgcgccat
    12541 taactataaa aagatgtatg acccggatca cgagtgctat catccgccct tatgcttagc
    12601 gcattaaccc caggggcaaa taatgaacca cgatatcccg ctaaaatatt ttgatattgc
    12661 cgatgagtac gcgaccgaat gcgcagagcc tgtcgcagac gctgaacgca caccgctggc
    12721 ccactacttc cagttactgc tcacccgttt gatgaacaat gaagagatca gcgaagaagc
    12781 ccaacatgaa atggctgctg aagcggggat taatcccgta cgcattgatg agatcgcaga
    12841 gtttttgaat caatggggta atgagtaatt atttaattgc agacgatcgg tcagtatcgt
    12901 tgtccggata aagcgttcac gccgcatccg gctaacaatg acacgcactg cagcgtgctt
    12961 taatgggttt ctcacgatcg taccgctctg ctcctcgcta agttgttctg tgagttatag
    13021 ttatagagga agcacgggta cgaatgttga acagatggcg acgccacctg ttcaacgacc
    13081 aaccacacgg ccctcattac gacccctgaa cacaaacggt aatggcaaat gtcattaaat
    13141 attagtaaga tagcctcttt acataaaatc ccttaatatt atgcgccagg tattatcgtc
    13201 tcttttggtg attgctggac ttgtgagtgg tcaggcaatc gccgcgcctg aatccccccc
    13261 gcatgctgat atccgcgaca gcggttttgt ctattgcgtc agcgggcaag tcaacacctt
    13321 taacccatcc aaagcgagca gtgggttaat tgtcgatacc cttgccgccc agttttatga
    13381 tcgactgctg gatgtcgatc cctataccta tcgcctgatg ccggaacttg ccgaaagctg
    13441 ggaagtactc gacaacggcg cgacctatcg cttccacctg cgtcgcgatg ttccgtttca
    13501 aaaaaccgac tggtttactc ccactcgtaa aatgaatgcc gacgatgtgg tgtttacctt
    13561 ccagcgaatt tttgaccgca acaacccgtg gcataacgtc aacggcagca acttccccta
    13621 cttcgacagc ctgcaatttg ccgataacgt gaaaagcgtc cgcaaactgg ataatcatac
    13681 cgttgagttc cgactcgccc agccggatgc ttcttttttg tggcacctcg caacccatta
    13741 tgcttcggtc atgtcggcag aatatgcccg gaagttagag aaagaagatc gccaggagca
    13801 actcgaccgt caaccggtcg gcaccggtcc gtatcagttg tcggaatacc gcgccgggca
    13861 atttattcgc ctacaacgtc atgatgactt ctggcgcggt aaaccgttaa tgccgcaggt
    13921 agtggtggat ttaggctccg gcggcaccgg acgtctgtcg aaactcctga ccggggaatg
    13981 cgacgttctg gcctggcctg ctgccagcca gctatccatt ttgcgtgacg acccgcgctt
    14041 gcgtttaacg ctgcgtcctg ggatgaacgt cgcctatctg gcatttaaca ccgccaaacc
    14101 gccgctaaat aatcccgctg tccgccatgc actggcactg gcgattaata accagcgcct
    14161 gatgcaatcc atctattatg gtacggctga aacggcggcc tctattttac cgcgcgcctc
    14221 gtgggcctat gacaacgagg ctaaaattac tgaatacaat ccggcgaaat cgcgcgaaca
    14281 gttgaaggcg ttggggctgg aaaacttaac gctgaaactg tgggtgccca cacgttcgca
    14341 ggcgtggaac cccagtccac tgaaaactgc cgaactgatt caggcggata tggcgcaggt
    14401 tggcgtaaaa gtggtgattg tgccggtaga aggtcgcttt caggaggcgc ggttgatgga
    14461 tatgagccat gatctgacgt tatccggttg ggcgacggac agtaacgacc cggacagttt
    14521 cttccgtcct ttactgagct gcgcggcaat tcattcacag accaacctcg cccactggtg
    14581 cgatccgaaa ttcgacagcg tgttgcgtaa ggcgctctcc tcgcagcagc tggcggcgcg
    14641 tattgaagcc tatgacgaag cgcagagtat tctggcgcag gaattgccca ttttgccgct
    14701 ggcgtcgtca ttgcgtttgc aggcctatcg gtacgatatc aaaggtctgg tacttagccc
    14761 gtttggtaac gcctcctttg ctggggtgta tcgcgagaaa caggatgagg tgaaaaaacc
    14821 atgattatct tcaccttacg ccgcattttg ttattgattg tcaccttgtt cctgctgacc
    14881 ttcgttggct tcagcttgag ctatttcacc cctcatgcgc cgttacaagg cgcgtcactg
    14941 tggaatgcct gggtgttctg gtttaacggc ctgatccact gggattttgg tgtttccagc
    15001 attaacggtc aaccgattgc cgaacagtta aaagaggtct tcccggcgac gatggaactg
    15061 tgcatcctcg ccttcggttt tgcgctgatt gttggcatcc ccgtggggat gattgccggg
    15121 attacgcgcc ataagtggca ggataacttg atcaacgcca tcgccttact gggcttttca
    15181 atcccggtgt tctggctggc gcttctgttg acgctgtttt gttcactcac gctaggttgg
    15241 ttgcccgttt cagggcgttt cgatctgctc tacgaagtga aaccgattac cggttttgcg
    15301 ttgattgatg cctggctttc ggactcacca tggcgggatg aaatgatcat gagcgcaatc
    15361 cgccatatga tattgcccgt gatcaccctg tcggttgcgc caacaactga agtgatccgc
    15421 ctgatgcgta tcagcactat cgaagtgtat gaccagaact atgtaaaagc ggcggcgacc
    15481 cgcggtttgt cgcgctttac tattttgcgt cgccacgttc tgcataacgc gttaccgccg
    15541 gttattcctc gcctgggctt acagttttcc accatgctga cgctggcgat gatcaccgaa
    15601 atggttttta gctggccggg tttagggcgc tggttaatta acgccatccg ccagcaggat
    15661 tacgcagcca tttccgccgg agtgatggtg tgtggctcac tggtcattat tgttaacgtg
    15721 atttctgata ttttgggtgc catggctaac cctctgaaac ataaggaatg gtatgcctta
    15781 cgatagcgta tacagcgaaa agcgcccgcc gggcacgctg cgtaccgcct ggcgcaaatt
    15841 ttatagtgac gcctctgcga tggtcggcct gtacggctgc gcggggctgg ctgtactgtg
    15901 tatttttggc ggctggtttg cgccttacgg catcgaccag caatttctcg gttatcaatt
    15961 attgccgcca tcatggtcgc gctatggcga agtttctttc ttcctgggga ctgacgacct
    16021 cggacgcgat gttttaagcc gtttactgag cggagctgcg ccaaccgtgg gtggcgcatt
    16081 tgtggtgacg cttgccgcga cgatctgtgg cctagtactg ggcaccttcg ccggggcgac
    16141 acacggcctc cgctcagcgg tgcttaacca tattctggat accttactgg cgatcccttc
    16201 gctgctgttg gcaattatcg ttgtagcgtt tgccggaccg agtttgtccc acgctatgtt
    16261 tgctgtctgg ctggcattgc tgccgcgtat ggtacgttcg atttacagca tggtgcatga
    16321 cgaactggaa aaagagtacg ttatcgccgc ccgtctggat ggcgcatcaa cgctgaatat
    16381 tctctggttt gctgtgatgc caaacatcac cgccgggctg gtcaccgaga tcacccgcgc
    16441 actgtcgatg gcaattctcg atatcgccgc gctgggcttt ctcgatctcg gcgcacaact
    16501 cccctcgcct gaatggggag cgatgctcgg tgatgcgctg gaactgattt atgtcgcccc
    16561 gtggactgtc atgctgccag gtgcggcaat tatgattagc gtgttgttgg ttaacctcct
    16621 tggcgatggc gttcgtcgtg cgattattgc gggggtggaa taatgccatt actcgatatt
    16681 cgtaacctga ccattgaatt taaaaccggt gatgagtggg ttaaagccgt cgaccgggta
    16741 agtatgacct taaccgaagg tgaaatccgc ggtcttgttg gtgaatccgg ttccggcaag
    16801 agtttgattg cgaaagcgat ttgtggggtg aataaagata actggcgtgt tattgctgac
    16861 cgtatgcgtt ttgatgatat cgatttgctg cgtctctccg cacgcgaacg gcgcaaactg
    16921 gttggccata acgtgtcgat gattttccag gaaccgcagt cgtgtcttga cccttcagaa
    16981 cgtgtgggtc gccagttgat gcaaaacatc ccagcctgga cctataaagg ccgttggtgg
    17041 cagcgttttg gctggcgcaa acgccgtgcc attgaactgc tgcaccgcgt ggggattaaa
    17101 gatcacaaag atgcgatgcg cagttttccc tatgagttga ccgaaggtga atgtcagaaa
    17161 gtgatgatag ccattgcact ggcgaatcaa ccgcgtctgc tgattgctga cgaaccgacc
    17221 aactcaatgg agccaacaac ccaggcgcaa atctttcgcc tgctgacgcg tctcaaccaa
    17281 aacagcaata ccactatttt gcttatcagc catgacttac aaatgcttag ccaatgggcg
    17341 gataaaatta acgtgcttta ctgcggtcaa acagtggaaa ccgcgccgag taaggagttg
    17401 gtgacgatgc cacatcatcc ttatacccag gcgctgatcc gcgcgatacc agacttcggc
    17461 agcgcgatgc cgcataaaag tcgcctcaat acgctgcccg gcgctatccc actgctggaa
    17521 cagttaccga ttggttgtcg tctggggcca cgttgcccgt atgcacaacg agaatgcatt
    17581 gtgacgccac gtttgacggg ggcgaaaaat catctctatg cctgtcattt cccgctgaac
    17641 atggagaaag agtgagatga tcgaaacgct gcttgaagtg cgtaatttaa gtaaaacgtt
    17701 ccgctaccgg accggctggt ttcgccgtca gaccgtagaa gcggtaaaac ccttgagctt
    17761 tacgctacgt gaaggccaga cactggcgat tattggcgag aatggttcgg gtaaatccac
    17821 gctggcaaaa atgctggcgg gaatgataga gcctaccagc ggcgagttat tgattgacga
    17881 tcatccactg cattttggcg attattcctt ccgcagtcag cgtattcgca tgatttttca
    17941 ggatccttcg acctcgttga atccccgtca acgtatctcg caaattctgg attttccact
    18001 gcgcctgaac accgacctgg aaccggaaca gcgtcgcaaa cagattattg aaacgatgcg
    18061 tatggttggg ctgctaccgg atcacgtcag ttactatccg catatgttgg cacccggaca
    18121 aaaacagcgt ctgggtctgg cgcgcgcgct gatattgcgc ccaaaagtca ttattgccga
    18181 tgaagcactc gcttcactgg atatgtcgat gcgttcgcag ttgattaatc tgatgctgga
    18241 gttacaggaa aaacagggca tttcgtatat ttatgttacc cagcatatcg gaatgatgaa
    18301 gcacattagc gaccaggtgc tggtgatgca tcagggcgag gttgtcgagc gaggcagcac
    18361 cgcggatgtg ctggcatcgc cgctgcatga actcaccaaa cggctgattg ccgggcactt
    18421 tggcgaagca ttgacagcgg atgcgtggcg taaagatcgc taattttctg aatcctcctt
    18481 ccagattatc cacctcattc tgacaagaga ttaaatcttg tcagaatgag gtgatggata
    18541 aaataaaatc aaatgcgcgt gatttaagac gcaatctcac tttgcaggaa cgaaagctct
    18601 ggcgatatct tcgcagccga cgttttggtg atttcaaatt tcgccgtcaa catccggtgg
    18661 ggagctacat tctcgatttt gcttgctgct cggcgcgcgt agtcgttgag ctggatggtg
    18721 ggcagcatga tttggcagtt gcctatgata ccaggcgcac tagctggctt gagtcgcagg
    18781 gctggaccgt gctgcgtttc tggaataacg agattgattg taatgaggag gcggtgctgg
    18841 agattattct gcaggaactg aaccgccgat caccctcacc ctgaaagggc gagggggccg
    18901 accgagccga atagctgttg tggtgaaaac atggagactg tgctggagaa tattcggcaa
    18961 ggtctgaacc atcccagcca tcgccataaa agggttaggg gctgtatgag cctgtttgtt
    19021 gctggggtaa caatatttgc acaatatggt cccctcgccc ctctggggag agggttagtg
    19081 taaggggaaa agcgcccacc ctgccgcagc ctgctccggt cggacctagc aactatagct
    19141 actcacagcc aggttgatta taataaccat ttatctgttc gtactgttta ctaaaacgac
    19201 gaatcgcctg attttcaggc acaacaagca tcaacaataa ggattaaagc tatgggtttt
    19261 ctttccggta agcgcattct ggtaaccggt gttgccagca aactatccat cgcctacggt
    19321 atcgctcagg cgatgcaccg cgaaggagct gaactggcat tcacctacca gaacgacaaa
    19381 ctgaaaggcc gcgtagaaga atttgccgct caattgggtt ctgacatcgt tctgcagtgc
    19441 gatgttgcag aagatgccag catcgacacc atgttcgctg aactggggaa agtttggccg
    19501 aaatttgacg gtttcgtaca ctctattggt tttgcacctg gcgatcagct ggatggtgac
    19561 tatgttaacg ccgttacccg tgaaggcttc aaaattgccc acgacatcag ctcctacagc
    19621 ttcgttgcaa tggcaaaagc ttgccgctcc atgctgaatc cgggttctgc cctgctgacc
    19681 ctttcctacc ttggcgctga gcgcgctatc ccgaactaca acgttatggg tctggcaaaa
    19741 gcgtctctgg aagcgaacgt gcgctatatg gcgaacgcga tgggtccgga aggtgtgcgt
    19801 gttaacgcca tctctgctgg tccgatccgt actctggcgg cttccggtat caaagacttc
    19861 cgcaaaatgc tggctcattg cgaagccgtt accccgattc gccgtaccgt tactattgaa
    19921 gatgtgggta actccgcggc attcctgtgc tccgatctct ctgccggtat ctccggtgaa
    19981 gtggtccacg ttgacggcgg tttcagcatc gctgcaatga acgaactcga attgaaataa
    20041 tcattctgtt ggtaaatatt gggcggcgtt ctgccgcccg ttatctctgt tatacctttc
    20101 tgatatttgt tatcgccgat ccgtctttct ccccttcccg ccttgcgtca ggataacgat
    20161 ttcctttacg accaaggagc gcccatggaa caacgccaca tcaccggcaa aagccactgg
    20221 tatcatgaaa cgcaatccag tactgcggag tatgacgttc tgcctctggt cccggaagcc
    20281 gcaaaggtca gcgatccctt tctgctcgac gtgatccttg atgaagaaac gctagctccc
    20341 ttcctttcat ggctggtccc tgcgcgcgtt cttgcagtgg aattgttccc tgaccagctt
    20401 accgtgaccc gttcacagac cttcaccgct tatgaacgct tgtctacggc cctgacggtt
    20461 gctcaggttt gcggcgtcca gcggttatgt aactactatt cggcgcgact tacgccgctc
    20521 cccgggcctg attcctctag ggaaagcaat cataggttgg cacaaatcac gcaatatgcc
    20581 cgccaactgg ctagctcgcc ttctattatc gacaaccgat cgcgccagca tctgaatgac
    20641 gtcggtctta ctgccaggga ctgtgtaatc attaaccaaa tcattggttt tattggcttt
    20701 caggcgcgga ccattgcgac atttcaggct tatctcgggc acccggtacg ctggttaccc
    20761 ggtctggaaa tacaaaacta cgccgacgcg tcactgtttg ctgatgaatc aatacgctgg
    20821 cgaagcagct atgaggtgga aaaactacct gaagagtaca caaaaagttc aacagcagaa
    20881 ctttgccaac tggccgaaac actctctctc caccctattt cactttccct tctcgaaagg
    20941 ttgttaaaca gtacacgggt taatacacag ccggataata agcttgcggc gttgttatgc
    21001 gcgcggataa atggcagtcc tgcttgtttt gccgcctgta tggattcatc aaatgaatat
    21061 aaaaaaatca gcccccttct gcgcaagggc gaaaatgaaa ttaaccaatg ggctgaccgt
    21121 cattctgttg agcacgctac cgttcaggcg atacaatggc tgacccgagc acccgatcgc
    21181 tttagcaccg cccagttcag ccctttactc gaacacgaac aatcatcaac gcagattatt
    21241 aatctgctgg tatggagcgg gctgtgtggc tggataaatc gcttaaaaat cgcgttgggt
    21301 gagacatatt aaccttgccg cgtcagacag attcgcgtaa aactgtcagc cgctctaatg
    21361 gccaccaaaa tagacaatta tgtttcagga caacccgctg ctagcgcagc ttaaacagca
    21421 actgcattcc cagacgccac gcgctgaagg ggtggtaaaa gccacagaaa aaggctttgg
    21481 cttcctggaa gtcgacgcgc aaaaaagtta tttcattccg ccgccgcaga tgaaaaaagt
    21541 catgcatggc gaccgaatta tcgcggtgat ccacagtgaa aaagaacgtg aatccgcaga
    21601 gccagaagaa ctggttgaac cgttcctgac tcgtttcgtg ggtaaagttc agggcaaaaa
    21661 tgaccgtctg gccatcgttc ctgatcatcc actcttaaaa gacgccattc cttgccgcgc
    21721 agcccgtggc ctgaaccacg agtttaaaga aggcgactgg gcggttgccg aaatgcgccg
    21781 tcatccgctg aaaggcgatc gttctttcta tgctgaattg acacaataca tcacttttgg
    21841 tgacgaccac tttgtaccgt ggtgggttac acttgcgcgc cataatctgg aaaaagaagc
    21901 accagacggc gtcgctaccg aaatgctcga tgaaggtctg gttcgtgaag atctgaccgc
    21961 gctggatttt gtcaccatcg acagtgccag cacagaagat atggatgacg cccttttcgc
    22021 taaggcgttg ccggatgaca aacttcagct gattgtggcg attgccgatc caaccgcgtg
    22081 gattgctgaa ggcagcaagc tggacaaagc cgcgaaaatt cgcgcattca ccaactatct
    22141 gcctggcttc aacatcccta tgctgcctcg cgagctttct gacgatctct gctcactgcg
    22201 cgccaatgaa gtccgcccgg tactggcatg ccgcatgacg ctctccgctg atggcgccat
    22261 tgaagataat atcgaattct ttgccgccac catcgaatcc aaagcaaagc tggtgtatga
    22321 ccaggtttct gactggctgg aaaataccgg tgactggcag cctgaaagtg aagcaattgc
    22381 cgaacaagtc cgtttgctag cgcaaatttg ccaacgccgc ggcgagtggc gtcataacca
    22441 cgcactggtg tttaaagatc gcccggatta ccgctttatt ctcggtgaaa aaggtgaagt
    22501 gctggatatc gtcgccgagc ctcgtcgcat tgccaaccgt atcgtcgaag aagcgatgat
    22561 tgccgctaac atttgtgcgg cccgcgtact gcgcgataag ctcggttttg gcatctataa
    22621 cgtgcatatg ggctttgatc cggcgaatgc cgacgcgctg gcagcgttgc tgaaaacgca
    22681 cggtctgcat gtcgatgccg aagaagtgct cacgctggac ggtttctgca aactgcgtcg
    22741 tgaactggac gcgcaaccaa ctggtttcct cgacagccgc attcgtcgct tccagtcatt
    22801 tgctgaaatt agcactgaac ccggtcctca ctttggcctc ggtctggaag catacgccac
    22861 ctggacttcg ccgatccgta aatatggcga catgatcaac caccgtctgc tgaaagcggt
    22921 tatcaaaggc gaaactgcga cgcgcccaca ggatgaaatc actgtccaaa tggccgagcg
    22981 tcgccgtctc aaccggatgg cagaacgtga tgttggtgac tggttatacg cacgcttcct
    23041 gaaagacaaa gccgggaccg acacccgttt cgcagcggaa attgtcgata tcagccgtgg
    23101 cggcatgcgt gtgcgtttgg ttgataacgg cgctatcgcc tttattccgg cacctttctt
    23161 acacgctatg cgcgatgaac tggtttgcag ccaggaaaac ggcactgtac aaattaaagg
    23221 tgaaacggtt tacaaagtaa ctgacgttat tgacgtcacc attgccgaag tccgcatgga
    23281 aacccgcagc attattgcgc gcccggtcgc gtaatctcct ttcacggcct attcctcatg
    23341 gatgggccgt ttatttcccc gctctacctt cgttatatcc aggcagattt tttaactgac
    23401 tttcgtttga aaactggcgt ttttccaaaa ctggattaca aataaatata taaaaaaaca
    23461 atgcgtttag ccgccgttaa tttcatgtaa gtca
//
LOCUS       DAZPEB010000044        23411 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZPEB010000044 DAZPEB010000000
VERSION     DAZPEB010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23411
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.045"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..1005
                     /locus_tag="ACHURJ_001550"
     CDS             <1..1005
                     /locus_tag="ACHURJ_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFR1280549.1"
                     /translation="PKSEKYVFIHGPENPDETWYYDFHHRRGVIAEGGKVSNLDAMDI
                     TAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNVGVAAPFGPVNVQ
                     KQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAFIGDTLSPKGEKV
                     PELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTHHRAYPGLVNVPR
                     HWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQSAFNWHPSGEWL
                     GFVLDNRIACAHAQSGEVEYLTESHANPPSADAVVFSPDGQWLAWMEGGQLWITETDR
                     "
     gene            complement(1007..1339)
                     /locus_tag="ACHURJ_001551"
     CDS             complement(1007..1339)
                     /locus_tag="ACHURJ_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFR1280550.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSHVEKSEANAQATNAVI
                     PPARMPDN"
     misc_feature    1579..1651
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            1645..2058
                     /gene="ibpA"
                     /locus_tag="ACHURJ_001552"
     CDS             1645..2058
                     /gene="ibpA"
                     /locus_tag="ACHURJ_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFR1280551.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            2170..2598
                     /gene="ibpB"
                     /locus_tag="ACHURJ_001553"
     CDS             2170..2598
                     /gene="ibpB"
                     /locus_tag="ACHURJ_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFR1280552.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            2794..4455
                     /locus_tag="ACHURJ_001554"
     CDS             2794..4455
                     /locus_tag="ACHURJ_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFR1280553.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAPALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(4452..5168)
                     /locus_tag="ACHURJ_001555"
     CDS             complement(4452..5168)
                     /locus_tag="ACHURJ_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR1280554.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            5463..7079
                     /locus_tag="ACHURJ_001556"
     CDS             5463..7079
                     /locus_tag="ACHURJ_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFR1280555.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMIWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGGWV
                     YTFLERILIPTDLHHFIYGQFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHALLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            7079..8402
                     /locus_tag="ACHURJ_001557"
                     /pseudo
     CDS             7079..8402
                     /locus_tag="ACHURJ_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
     gene            complement(8399..8473)
                     /locus_tag="ACHURJ_001558"
                     /pseudo
     CDS             complement(8399..8473)
                     /locus_tag="ACHURJ_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8533..8964)
                     /gene="tnpA"
                     /locus_tag="ACHURJ_001559"
                     /pseudo
     CDS             complement(8533..8964)
                     /gene="tnpA"
                     /locus_tag="ACHURJ_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            8972..10166
                     /locus_tag="ACHURJ_001560"
                     /pseudo
     CDS             8972..10166
                     /locus_tag="ACHURJ_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
     gene            10337..10684
                     /locus_tag="ACHURJ_001561"
     CDS             10337..10684
                     /locus_tag="ACHURJ_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFR1280556.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            10674..11036
                     /locus_tag="ACHURJ_001562"
     CDS             10674..11036
                     /locus_tag="ACHURJ_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFR1280557.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            11033..11530
                     /locus_tag="ACHURJ_001563"
     CDS             11033..11530
                     /locus_tag="ACHURJ_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFR1280558.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGGENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(11538..12722)
                     /gene="emrD"
                     /locus_tag="ACHURJ_001564"
     CDS             complement(11538..12722)
                     /gene="emrD"
                     /locus_tag="ACHURJ_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFR1280559.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCVGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFLFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            12804..12878
                     /gene="ysdE"
                     /locus_tag="ACHURJ_001565"
     CDS             12804..12878
                     /gene="ysdE"
                     /locus_tag="ACHURJ_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFR1280560.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            complement(13141..13230)
                     /gene="tisB"
                     /locus_tag="ACHURJ_001566"
     CDS             complement(13141..13230)
                     /gene="tisB"
                     /locus_tag="ACHURJ_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFR1280561.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            13795..13893
                     /gene="ivbL"
                     /locus_tag="ACHURJ_001567"
     CDS             13795..13893
                     /gene="ivbL"
                     /locus_tag="ACHURJ_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFR1280562.1"
                     /translation="MTTSMFNAKLLPTALSAAVVVVRVVVVVGNAP"
     gene            13999..15687
                     /gene="ilvB"
                     /locus_tag="ACHURJ_001568"
     CDS             13999..15687
                     /gene="ilvB"
                     /locus_tag="ACHURJ_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFR1280563.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            15691..15981
                     /gene="ilvN"
                     /locus_tag="ACHURJ_001569"
     CDS             15691..15981
                     /gene="ilvN"
                     /locus_tag="ACHURJ_001569"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFR1280564.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            16055..16645
                     /gene="uhpA"
                     /locus_tag="ACHURJ_001570"
     CDS             16055..16645
                     /gene="uhpA"
                     /locus_tag="ACHURJ_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFR1280565.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            16645..18147
                     /gene="uhpB"
                     /locus_tag="ACHURJ_001571"
     CDS             16645..18147
                     /gene="uhpB"
                     /locus_tag="ACHURJ_001571"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFR1280566.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            18157..19476
                     /gene="uhpC"
                     /locus_tag="ACHURJ_001572"
     CDS             18157..19476
                     /gene="uhpC"
                     /locus_tag="ACHURJ_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFR1280567.1"
                     /translation="MLPFLKAPADAPLMTDKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            19614..21005
                     /gene="uhpT"
                     /locus_tag="ACHURJ_001573"
     CDS             19614..21005
                     /gene="uhpT"
                     /locus_tag="ACHURJ_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFR1280568.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(21050..22816)
                     /gene="adeD"
                     /locus_tag="ACHURJ_001574"
     CDS             complement(21050..22816)
                     /gene="adeD"
                     /locus_tag="ACHURJ_001574"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFR1280569.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYITAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLDL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVYQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFVFTTLEVTE"
     gene            22991..>23411
                     /gene="adeQ"
                     /locus_tag="ACHURJ_001575"
                     /gene_synonym="yicO"
     CDS             22991..>23411
                     /gene="adeQ"
                     /locus_tag="ACHURJ_001575"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFR1280570.1"
                     /translation="MNNDNTDYVSNESGMLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISN"
BASE COUNT         5355 a         5981 c         6368 g         5707 t
ORIGIN      
        1 ccgaagtcag agaaatatgt tttcatccat ggcccggaaa atcctgatga aacatggtat
       61 tacgatttcc atcatcgacg cggagtcatt gctgaaggcg gcaaggtgag caatctcgat
      121 gcaatggata ttaccgcgcc gtatacgcca ggagcgctgc gcggcggcag ccatgtgcat
      181 gtctttagcc cgaacggtga aagggtgagc tttacctata acgaccatgt aatgcatgaa
      241 ctcgatccgg cgctggattt gcgaaacgtc ggtgttgctg cgccgtttgg cccggtcaac
      301 gtacaaaagc agcatccgcg tgaatacagc ggtagccact ggtgcgtgct ggtgagtaaa
      361 accacgccca cgccgcagcc tggcagcgat gaaatcaatc gtgcttatga agaaggatgg
      421 gtaggaaatc acgcgctggc gtttattggc gacacacttt cgccaaaggg cgagaaagtg
      481 ccggagctgt ttatcgttga gttaccgcaa gatgaagctg gctggaaagc ggcaggtgat
      541 gcgccgttaa gtggaacgga aaccaccctg cccgcgccac cgcgcggcgt cgtgcagcga
      601 cgtttaacct ttacccacca tcgggcttat ccggggttag tcaacgtccc gcgccactgg
      661 gtgcgctgta atccgcaggg tacgcaaatc gcgtttttaa tgcgtgatga taacggcatt
      721 gtgcaactgt ggcttatctc gccacagggc ggcgagccgc gccagttaac ccataacaaa
      781 acggatattc agtctgcatt taactggcat ccgtcaggag aatggttggg ctttgtgctg
      841 gataatcgaa ttgcttgtgc ccatgcgcaa agtggcgagg ttgagtattt aaccgaaagc
      901 cacgccaatc caccttctgc ggacgccgtg gtcttctcgc cggatggtca atggctggcg
      961 tggatggaag gcggccagct gtggatcacc gaaactgatc gctaaatcag ttatcaggca
     1021 ttctggccgg tggaataacc gcattggtgg cttgcgcgtt ggcttcgctt ttttcaacgt
     1081 gtgagcgcac cgagctgtct ttgcggaaca catcccacgg cagtaaaagc gtgtccatca
     1141 cagcggtaaa cggcatatcg agaatcgcca gcgacttagt gccccagttg gtttcatcgt
     1201 cgccaatcat ggttgcgctg gcgcgggtgc cgggatacgt tccttctttc cctccggtat
     1261 gggacatcac gctggagcat cctccgagta atgtcattcc gctgcatatc atgaacgcta
     1321 acaacacatt tcttatcata ataaattcat ctgttgatcg tgggtgttgg cctgatgagt
     1381 tatagcgatc ccttgctgaa aataacatca tcattacgtc gcactgtggc ggctatcgca
     1441 ctttaacgtt tcgtgctgcc ccctcagtct atgcaataga ccataaactg caaaaaaaag
     1501 tccgctgata aggcttgaaa agttcatttc cagacccatt tttacatcgt agccgatgag
     1561 gacgcgcctg atgggtgttc tggctacctg acctgtccat tgtggaaggt attacattct
     1621 cgctgatttc aggagctatt gattatgcgt aactttgatt tatccccgct ttaccgttct
     1681 gctattggat ttgaccgttt gtttaaccac ttagaaaaca accagagcca gagtaatggc
     1741 ggctaccctc cgtataacgt tgaactggta gacgaaaacc attaccgcat tgctatcgct
     1801 gtggctggtt ttgctgagag cgaactggaa attaccgccc aggataatct gctggtggtg
     1861 aaaggtgctc acgccgacga acaaaaagag cgcacctatc tgtaccaggg catcgctgaa
     1921 cgcaactttg aacgcaaatt ccagttagct gagaacattc atgttcgtgg ggctaacctg
     1981 gtaaatggtt tgctgtatat cgatctcgaa cgcgtgattc cggaagcgaa aaaaccgcgc
     2041 cgtatcgaaa tcaactaatt ccctaaggcc gcctgacgcg gcctgacatc tccatgctcg
     2101 ccgtcaggga gcatatgcga atcttcggat ttgcaggtac ttactcgctt cttagaagga
     2161 gaaatgacta tgcgtaactt cgatttatcc ccactgatgc gtcaatggat cggttttgac
     2221 aaactggcca acgcactgca aaacgccggt gaaagccaga gcttcccgcc gtacaacatt
     2281 gagaaaagcg acgataacca ctaccgcatt acccttgcgc tggcaggttt ccgtcaggaa
     2341 gatttagaga ttcaactgga aggtacgcgc ctgagcgtaa aaggcacgcc ggagcagcca
     2401 aaagaagaga aaaaatggct gcatcaaggg cttatgaatc agccatttag cctgagcttt
     2461 acgctggctg aaaatatgga agtctctggc gcaaccttcg taaacggttt actgcatatt
     2521 gatttaattc gtaatgagcc tgaacccatc gcagcgcagc gtatcgctat cagcgaacgt
     2581 cccgcgttaa atagctaact agctattctc tttgccccgc cattctggtg gggctttttt
     2641 tgtctttctt cttcaacaat gcgttaaatt tcgactgttt aagatatttc ggcacgttct
     2701 ttcccgtgat aatgtgcgct ataaggcaaa tctgcttcac cgattcagcc ataatccgta
     2761 gcaattaaaa tgttatctca agagaaatgg acgatgagtg atatagcatt aacggtcagt
     2821 attctggctt tggtggcagt cgtcggtttg tttatcggca acgtcaaatt tcgcggcata
     2881 ggattaggta ttggcggcgt gctgtttggt gggatcatcg tcggccattt tgtttctcag
     2941 gcggggatga cattaagtag cgatatgctg catgttattc aggaatttgg cctgatcctg
     3001 ttcgtttata ccatcgggat tcaagtaggg ccgggcttct ttgcctcatt gcgcgtctcc
     3061 ggcttacgcc tcaacctgtt tgctgttctg atcgtcatca tcggtggtct ggttaccgcc
     3121 atcctgcata aactgtttga tattccactg ccggtagtgc tggggatttt ctccggtgcg
     3181 gttaccaata cgccagcgct gggggcaggg cagcagatct tgcgcgacct gggtacacca
     3241 atggaaatgg tcgatcagat ggggatgagt tacgcgatgg cgtatccatt cggcatttgc
     3301 gggattttgt tcaccatgtg gatgttgcgg gttattttcc gcgtcaatgt cgagacagaa
     3361 gctcagcagc acgagtcttc acgcaccaat ggcggcgcgc tgatcaagac tatcaatatt
     3421 cgcgttgaga accctaacct gcatgattta gctattaaag atgtaccgat tctcaacggc
     3481 gacaaaatta tctgctcgcg tctgaaacgc gaagaaaccc taaaagttcc ttcgccagat
     3541 accattatcc aactgggcga tttgctgcat ctggtggggc agccagcgga tttacataat
     3601 gcgcaactgg tgattggtca ggaggtcgat acttcgctgt ccacgaaagg cactgatttg
     3661 cgcgtcgagc gtgtggtggt caccaatgaa aacgtgctcg gaaaacgtat tcgcgacctg
     3721 cactttaaag aacgctatga cgttgttatc tcgcgcctga accgtgccgg ggtcgaactg
     3781 gtcgccagtg gcgatatcag cctgcagttc ggcgatatcc tcaatctggt ggggcgtccg
     3841 tccgcaattg atgccgttgc caatgtgctg gggaatgcgc agcaaaaact gcaacaggtt
     3901 cagatgctgc cagtgtttat tggcatcggg cttggcgtat tgttaggttc tattcccgtc
     3961 tttgtgcctg ggttcccggc cgcgttgaaa ctggggctgg cgggcggtcc gctgattatg
     4021 gcgttgatcc tcgggcgtat cggcagtatc ggcaagctgt actggtttat gccgccaagt
     4081 gccaacctcg cgctgcggga gctggggatc gtgctgttcc tctcggtcgt tggtctgaaa
     4141 tctggtgggg attttgtgaa taccctggtc aatggcgaag ggctaagctg gattggttat
     4201 ggtgccctga tcaccgccgt tccgctgatt actgttggca ttctggcgcg gatgttagcc
     4261 aaaatgaatt acctgaccat gtgcgggatg ctggcaggtt ccatgaccga tcctccggcg
     4321 ctggcgtttg ctaataatct tcatccaacc agtggtgcgc cggcgctctc ttacgccact
     4381 gtctatccgt tagtgatgtt tctgcgaatt atcacccccc aattactggc ggtgctcttc
     4441 tggagtatcg gttaactctt ttccgggtgg agtcgccgta aatggtactc cacctggtat
     4501 tcgctggcat ttctgaacat cactgaataa ttcaaaaact ccccgctctc gctatatgac
     4561 aacgaggtga tccgcagcag tggcgtgtgt tccgctacct tcatttgccg cgccagcagt
     4621 ctatcggcga gcaccggcgt caggctttca taattaccgc caatcaaaat cccacactct
     4681 tgttcaatat attcaaactt cgacccttcc agatgttgca gcgaaagatt acggaacagt
     4741 ttaaccggca tatagctgtc ttccagcatc agcggtttcc cttccacaaa acgaacgcga
     4801 cgggagaagt agatctgctc gttgatttga atccgtaact ggctggcaat ggccggaggc
     4861 gcaggcatga tttcaaaaat cagcacctgg ctggtgaccg tttttccctg ccgttttaac
     4921 acctccacca gtccggtcag gctggcggtt tgatgcagca catctttgcg caccaggtaa
     4981 gtgccgctgc cgtggcggcg gaccaccagc ccccacgcta ccagcaggtc aatcgcttta
     5041 cggatggtca tccgggatac tgcaaattct tccgccaact ttttttcacc gggaagaagg
     5101 ctgtttagcg tgaaatccgc ggaattaagt cgaattctta accgctccgc aatgcttttg
     5161 tagatcatct ctagatctct tttttgcctg caagcccggg taatgcgccc acaagttctg
     5221 cttaatcgat tgaaaaaaca tcttttttta aagatgtgtt cgatgctgtg taccagtgct
     5281 cacagatgtc tactttttcg cgaaaacgta gatctctacc gcccaacgaa aagcatgaaa
     5341 gcgatcacga atcccattgg gtcgtcatgt tctgccgatc gcatcttcct atcctcgctc
     5401 caggcctgcc gcataaccaa tcaggcttcc tacttacaga attgagaaaa gaggatgtgg
     5461 aaatgctcag tcaaattcaa cgctttggcg gcgcgatgtt cacgccagtg ctgctgtttc
     5521 ccttcgccgg gattgtggtg ggtcttgcca tcttgctgca aaacccgatg tttgtcgggg
     5581 aatcactgac cgatccgaac agtttattcg cgcaaatcgt acacattatt gaagagggcg
     5641 gttggacggt attccgtaat atgccgctga tttttgctgt cggtttaccc attggccttg
     5701 ctaagcaagc gcaggggcgt gcttgtctgg cggtgatggt gagtttcctg acctggaact
     5761 atttcatcaa cgcgatggga atgatctggg gaagctactt cggcgtcgat ttcactcagg
     5821 acgcggtggc aggtagcggt ctgacaatga tggccgggat taaaaccctc gataccagca
     5881 ttatcggcgc aattatcatt tccggcattg tgacggcgct gcataaccgt ctgttcgata
     5941 aaaaactgcc ggtttttctc ggcattttcc aggggacgtc ttatgtggtg attatcgcct
     6001 tcctggtgat gatcccctgt gcctggctga cgttgctcgg ttggccaaaa gtacaaatgg
     6061 ggattgaatc tctgcaagcg ttcctgcgtt cggcgggtgc gcttgggggg tgggtttaca
     6121 ccttcctcga acgtattctg atcccaaccg atttacacca cttcatctac ggacagttta
     6181 tctttggtcc ggcagctgtt gaaggcggca ttcagatgta ctgggcgcag catctgcaag
     6241 agttcagtct gagcgccgag ccgctgaaat cgttgttccc ggaaggcggt tttgccctgc
     6301 acggtaactc aaaaatcttt ggtgccgtgg gcatttcttt agcgatgtac ttcactgccg
     6361 caccggaaaa tcgggtaaaa gtggcgggct tgctgattcc cgcaacctta accgccatgc
     6421 tggtgggaat taccgaaccg ctggaattta ccttcctgtt catttcaccg ttgctgtttg
     6481 cggtacacgc cttgctggcg gcctcaatgt cgaccgtgat gtatctcttt ggtgtggtgg
     6541 gcaacatggg cggaggtctg attgaccagg ttttaccgca aaactggatc ccgatgttca
     6601 gcaaccacgc ggatatgatg ctgacccaaa tcgccattgg gttgtgcttt accctgctgt
     6661 acttcgtggt tttccgcaca ctgattctgc aattcaacat gtgcacgccg ggacgtgaag
     6721 atgcggaagt gaaactctac tcaaaagccg aatacaaagc ctcgcgaggc caaaccaccg
     6781 ctgcagagcc aaaaaaagag ctggatcagg ctgccggtat cctgcaagcc ctgggcgggg
     6841 tcggcaatat ctccagcatt aacaattgcg cgacgcgttt acgtattgca ctgcatgaca
     6901 tgtcacaaac gctggatgac gaagtcttta aaaagctggg agcgcacggc gtcttccgta
     6961 gtggcgatgc cattcaggtg atcattggtc tgcatgtatc ccagctgcgt gaacagctcg
     7021 atagcttaat taattctcat caatcagcag aaaatgttgc cattacggag gcagtataat
     7081 gaccaaattc tcagtggttg tcgcaggcgg tggaagcacc tttacgccag gcatcgtgtt
     7141 gatgctcctg gcgaatcagg accgtttccc gcttcgtgca ctgaaatttt atgataacga
     7201 tggtgcgcgg caggaagtga ttgccgaagc ctgtaaagtc atccttaaag agaaagcgcc
     7261 ggacattgcg tttagttaca ccaccgatcc tgaagtggca ttcagcgacg ttgattttgt
     7321 catggcgcac atccgcgtcg gcaaataccc gatgcgcgaa ctggatgaaa aaatcccgct
     7381 gcgccacggc gttgttggtc aggaaacttg cggacccggc ggaatagcgt acggcatgcg
     7441 ttccattggc ggcgtcctgg aactggtgga ttatatggaa aaatattcac caaatgcctg
     7501 gatgctcaac tactccaacc cggcagcaat tgtcgcagaa gccacgcgtc gtctgcgccc
     7561 gaatgcgaaa atcctcaaca tctgtgacat gccaatcggt attgaaagcc ggatggcgca
     7621 aattgttggg ctgcaagatc gcaaacagat gcgcgtgcgc tactacggcc tgaaccactt
     7681 tggctggtgg acatcaattg aagatttgca gggcaacgac ctgatgcccc agctgcggca
     7741 atatgtctct aagcacggtt atgttccacc gcagcaagat acacatactg aagcgagctg
     7801 gaacgacacc tatgcaaaag cgcgggatgt ccaggcactg gacccggata cattgccgaa
     7861 cacctatctg aaatattatc tcttcccgga ttatgttgtt cagcattcca accctgaaca
     7921 tacccgcgcg aatgaggtga atggaacatc gcgagaaaca ggttttcgat gcttgccgcg
     7981 ccattacagc ggcaggaaat tcagcggcgg ccaagctgga gattgacgaa catgcgtcat
     8041 acatcgtcga tctggcggcg gcaattgcct tcaacactca ggagcggatg ttgctgattg
     8101 tgcctaacaa cggggcaatc cataactttg atgatgaagc gatggtcgag atcccgtgtc
     8161 tggtcgggca caacggacca gaaccactgg tggtcggcga tatcccgcag tttcagaaag
     8221 ggttaatgag tcagcaagtg gcggtggaaa aactggtcgt ggacgcctgg gaacagcgtt
     8281 catatcagca cctgtggcag gcgattacgt tatcgaaaac ggtaccgagc gcctcggtcg
     8341 ccaaagctat tctggatgag ttgctggagg ccaacaaagc gtactggcca gagttacgtt
     8401 aattgtgtgg actggcatcc gttgggtgct ggtccgtatt ctttagcacg gctgacggcg
     8461 cataaccaaa tttatccggt gcgccgtaaa accccgtcct tcagggcggg gatataaggc
     8521 gcggttttcc acctaaccag gtgtttgctg ttgttcaatg tattggcgga tgacggatat
     8581 tggcgcacct ccgcagctat tggcaaaata gccaggactc cacaaaacgc ctttgtagta
     8641 atacctgact gcaatatctg gtcgatctcg tcgcagtaac ctacccgata cgcctttgag
     8701 gctgtttacc agactggata tcgccagttt gggaggatag ttaattaaca aatggacgtg
     8761 atctggttcg ccatccattt aaaccagttc agcttcaaaa tcagcacata catttgaaaa
     8821 gtaagtgcgt agtttttctg tcgcgtcata gtcaaaaatc tgacgtcggt atttggtgac
     8881 aaagaccagg tgaacatgca tcaggaaaac acaatgcctt ccacgccgga tatcggtttc
     8941 ttttttcaca gaccaaagta tcatttggcc tgtgaaacga ctacaagcat ttaaattcca
     9001 gttaagaccc ggtggtcaac aggagcgtga aatgaggcgc ttcgcaggcg catgtcgttt
     9061 cgttttcaat cgtgctctgg cacgtcagaa tgagaatcat gaggccggta ataaatacat
     9121 cccttacggg aaaatggctt cctggctggt tgagtggaaa aatgccactg aaacgcaatg
     9181 gcttaaagat gccccgtcac agccattgca acagtcactg aaagaccttg agcgggctta
     9241 caaaaacttc ttccagaatc gggctgcttt tccccgattc aaaaagcggg gacagaatga
     9301 tgtattccgc tacccgcagg gcgttaagct cgatcaggaa aacagccgta tttttctgcc
     9361 gaaactgggc tggatgcgct accggaacag ccgttaggtc acgggtgttg tgaaaaatgt
     9421 cactgtcagc cagtcctgcg gtaagtggta catcagtatt cagacagaaa gtgaagtatc
     9481 aactccggtt cacccttcag catcaatggt cgggctggat gctggcgtgg ctaaactcgc
     9541 cacgctgtca gatggcacag tctttgagcc tgtaaacagt tttcagaaaa accagaagaa
     9601 gctggcgaga cttcagcgac agttaagccg caaggtcaaa ttcagcaaca actggcagaa
     9661 gcagaaacgc aaaatacagc gactgcattc ctgtatcgca aatatccgca gggactacct
     9721 tcacaaagtc acaacgaccg tcagcaaaaa ccacgcaatg atagtcattg aggatttgaa
     9781 ggtcagcaac atgtcaaagt cagcagcggg tacggtcagc ctgccggggc gcaatgtccg
     9841 ggcaaaatca ggtttaaacc gttcgatact ggatcagggc tggtatgaaa tgcgccgcca
     9901 gcttgagtac aagcagctct ggagtggcgg tcaggtgctt gctgttccgc cagcgttgcg
     9961 cgtgctgtgg tcatacagcg aaagaaaatc gcctgtcaca aagtaaattc agatgccagg
    10021 tatgtggata tacagcgaac gccgatgtaa atggcgctcg taacatttta gcggcggggc
    10081 acgccgttct tgcctgtgga gagatggtgc agtcaggccg cccgttgaag caggaaccca
    10141 ccgaaatgat tcaggcgaca gcctgaacgt agcagggatc ctcgtccttc agggcgtgga
    10201 ggatgtcaat ttgcgctatg atacccatct gacctccttg tagcggatgg caaaagcata
    10261 tcaggaaacg ggcggacgaa attcttaaaa aaaaccgcta aagatagagc gttagtgaaa
    10321 ttgcaaagga gcaacaatga aaatttcccg ccttggagaa gcgcccgatt accgcttctc
    10381 gctggctaat gagcgtactt ttctggcgtg gatccgtacc gcactaggat ttctggcggc
    10441 gggggttggg cttgatcaac ttgcgccaga tttcgccacg cccgtcattc gtgaactgct
    10501 ggcgttgttg ttgtgtctgt tttccggcgg gttagcaatg tatggctacc tgcgctggtt
    10561 acgcaatgaa aaagcgatgc gtctgaaaga ggacctgccc tacaccaaca gcttattaat
    10621 cattagctta atattgatgg tcgtggcggt gatcgttatg ggactggtgt tgtatgccgg
    10681 atagccgcaa agccagacgc attgccgacc caggactgca gccggagcgc acatcactgg
    10741 cgtggtttcg taccatgctg ggctacggcg cgttgatggc gttggctatc aaacacaact
    10801 ggcaccaggc gggcatgtta ttctggattt cgattggcat tctcgccatc gtggcgctga
    10861 tcctctggca ctacacccgt aatcgcaatt taatggatgt cacgaatagc gatttttccc
    10921 aatttcacgt agtccgtgac aaatttttga tctccctcgc ggtgttatct ctcgcaatac
    10981 tgtttgctgt aacgcatata catcaactta tcgtatttat tgagagagtc gcatgacagg
    11041 aagtcaggtc attgatgctg aagaggacag gcataaactg gttgtagagt acaaggatgc
    11101 tctgcaacct gccgattttt atcacaattt caaacagcgg ggcatccgct ccgtacagct
    11161 cattccctat cttgaatttg atgaccgggg cgatctgacg gccgcttcgg tgacagcaga
    11221 gctgtggggg aaatttttaa ttgccctgtt cgagtgttgg gtgcgggcag atatcagtcg
    11281 tatctcgatt gagctttttg acgccacctt acaaaaatgg tgtggcggcg aaaacccgca
    11341 accgcgccgt gactgccagg cgtgcgactg gcatcgcctg tgcccgcacg ctcggcagga
    11401 gacgccagac agcgtgcttt gtgcgggcta tcaggcgttt tattcttact ccgcaccgca
    11461 tatgcgggtg atgcgtgatc tgataaagca gcatcgttct cccatggagc tgatgacgat
    11521 gctgcggtga cgtgcgctta aacgggctgc ccctgatgcg acatccgcgt cgccagcggc
    11581 agccagcaca gcacgatcaa caatcccatt aaggtcatca ataaccccag gctaccctga
    11641 ccggtttgcg gcaacatcgc agagagcgac gccagcacgc cggaaccaat gttttgcaga
    11701 ccgccgacca gcgcgccagc cgtgcccgcc aggaagagga acggctccat cgcgccgctg
    11761 gtcgccagcg gaaacagcat cccggcaccg aaaaagaaca gcgcggcggg aacgagcagc
    11821 gtccagacat tcatcacgcc aaaccagtcg gggatccaca tcagcaagcc agccagcagg
    11881 cagcagataa ccgactgcca cattaacgtg gagaagcgtt tattgggacg tccggcaaac
    11941 catgcgccaa aaaacgctgc cggaatcggc agaataaaca aaatactgac cgtcatactg
    12001 ctcagcccta acaccgcgcc catcagcacg ccggagcagg cttcaaaggc ggcaatcccg
    12061 gccagaccgc caatcagcat cagcaaataa cagttaaaac cgctgttacc gaaaagcgtt
    12121 ttataactgg taagcaggcg cgtgcgcggt gcatcgaccg gacgcgtttc cggcatccag
    12181 cgggccatac tgaaggtcac accaacacaa agaaccaaca aaaagagata acaggcgcgc
    12241 cagttccaca tcgtatccag cagaccgccg attagcggtg cgagcaacgg actgacgaga
    12301 atccccatgt ttaacaggct gttagcatgg cgcaactgtg tccgttcata taaatctcgc
    12361 ggcaaagtac gcgccattac gccgccaacg ccggtgccca tcccctgcat cgcgctggcg
    12421 gcaatcaata ccgtcaaact ggaggtcgtg accgcgacca gcgttgccag cataaaaatg
    12481 gacattccga cgaggatcac cggtcggcga cccacacggt cggaaatcgg gccataaaac
    12541 agctgtgaga caccgtaagt cagcagataa gcgcccatta cgctctgcac cgccccttca
    12601 cggacgttga gatcgcgcgc catatcggca atagctggaa tataaatggt ttgcgccatc
    12661 tgaccgacgg ccacgagtaa taccaacatc aataacaaat tgacgtttct ttgccttttc
    12721 attatcacgg atgcttttat aaaaataatt agaataagtc actacgcatt cccataaatg
    12781 cgggagagga atctatcaca ttgatgaatg ttagccagat atacgccagg aatggggaat
    12841 tgtttagcgg cagaatatgt aaacaaaagc ggcaataaat gttgccggga gggattacgt
    12901 gcgaactgta accctctccc caaaggggcg agggggaaac caccgttccc tttcagaaaa
    12961 tgagctaaac attgcacggg acggtcccct cgcccctttg gggagagggc tagggtgagg
    13021 ggcacaggca gcgcaggtgc aaacattctg ctggtagaag aagcccccaa ctccaacccg
    13081 cacgctaaat ataaaagggg agcggttacc cgctcccctt tggtgcgact tgaatctgaa
    13141 ttacttcagg tatttcagaa cagcatcaag cagttgcagt gctgcaacaa tgagtttgag
    13201 gataagaatg gcgatatcca ccaggctcat acgcgtctcc tgtggttcag gagcactgcc
    13261 agctgacgta cctttccgct gcctgttgcc agctgttgtg cacgcggtat ctcaaggaga
    13321 aacgttgtgc ttgttgacat aacacagtgt gctcacgcgg ggttaaggcg ctgacggcac
    13381 cacccgtttc agccaggact tagtgcgccg ggtaacgatg cggcccccgc ataatacatt
    13441 gcgtacagtg atattataga agtttactgt ataaataaac agtgatattt ggacaaaacg
    13501 caaactgtga tcgatactca actatgccct aaatacgtca aaattcgtgc cgaaattgcg
    13561 cgttctgcgc ggaacacgta tactttcagt gttgacataa tacagtgtgc tttgcggtta
    13621 ccagctgcag gcggctgatg aaacctcgct ccggcggggt tttttgttac ctgcaattca
    13681 gtacaaaacg tgatcagccc ctcaattttc ccttgctgaa aaattttcca ttgtctcccc
    13741 tgtaaagctg tgcttgtata aatattgtta aacacaaaac caacaaggtc cccaatgact
    13801 acttccatgt tcaacgcaaa actactacca actgcgctat ccgccgcagt ggtcgtcgtg
    13861 cgtgtggtgg tggtcgtcgg caatgcgccg tagggactgg aacaacacac gattccaaaa
    13921 ccccgccggc gcaaaccggg cggggttttt cgtttaagca cctcccggaa agtcggccca
    13981 gaagaaaagg actggagcat ggcaagttcg ggcacaacat cgacgcgtaa gcgctttacc
    14041 ggcgcagaat ttatcgttca tttcctggaa cagcagggca ttaagattgt gacgggcatt
    14101 ccgggcggtt ctatcctgcc tgtttacgat gccttaagcc aaagcacgca aatccgccat
    14161 attctggccc gtcatgaaca gggcgcggga tttatcgctc aggggatggc gcgcaccgac
    14221 ggtaaaccgg cggtctgtat ggcctgtagc ggaccgggtg cgactaacct ggtgaccgcc
    14281 attgccgatg cgcggctgga ctccatcccg ctgatttgca tcaccggtca ggttcccgcc
    14341 tcgatgatcg gcaccgacgc cttccaggaa gtggacacct acggcatctc tatccccatc
    14401 accaaacaca actatctggt cagacatatc gaagaactcc cgcaggtcat gagcgatgcc
    14461 ttccgcattg cgcaatcagg ccgcccaggc ccggtgtgga tagacattcc taaggatgtg
    14521 caaacggcag tttttgagat tgaaacacag cccgctatgg cagaaaaagc cgccgccccc
    14581 gcctttagtg aagaaagcat tcgtgacgca gcggcgatga ttaacgctgc caaacgcccg
    14641 gtgctttatc tgggcggcgg tgtgatcaat gcgcccgcac gggtgcgtga actggcggag
    14701 aaagcgcaac tgcctaccac catgacttta atggcgctgg gcatgttgcc aaaagcgcat
    14761 ccgttgtcgc tgggtatgct ggggatgcac ggcgtgcgca gcaccaacta tattttgcag
    14821 gaggcggatt tgttgatagt gctcggtgcg cgttttgatg accgggcgat tggcaaaacc
    14881 gagcagttct gtccgaatgc caaaattatt catgtcgata tcgatcgcgc agagctgggt
    14941 aaaatcaagc agccgcatgt ggcgattcag gcggatgttg atgacgtgct ggcgcaattg
    15001 atcccgcagg tggaagcgca accgcgtgca gagtggcacc agttggtagc ggatttgcag
    15061 cgcgaattcc cgtgtccaat cccgaaagcg tgcgatccat taagccatta cggcctgatc
    15121 aacgccgttg ccgcctgtgt cgatgacaat gcgattatca ccaccgatgt gggtcagcat
    15181 cagatgtgga ccgcgcaagc ttatccgctc aatcgcccac gccagtggct gacctccggt
    15241 gggctgggca cgatgggttt tggcctgcct gcggcgattg gcgcggcgct ggcgaacccg
    15301 gatcgcaaag tgttgtgttt ctccggcgac ggcagcctga tgatgaatat tcaggagatg
    15361 gcgaccgcca gtgaaaatca gctggatgtc aaaatcattc tgatgaacaa cgaagcgctg
    15421 gggctggtgc atcagcaaca gagtctgttc tacgagcaag gcgtttttgc cgccacctat
    15481 ccgggcaaaa tcaactttat gcagattgcc gccggattcg gcctcgaaac ctgtgatttg
    15541 aataacgaag ccgatccgca ggctgcattg caggaaatca tcaatcgccc tggcccggcg
    15601 ctgatccatg tgcgcattga tgccgaagaa aaagtttacc cgatggtgcc gccaggtgcg
    15661 gcgaatactg aaatggtggg ggaataagcc atgcaaaaca caactcataa caacgtaatt
    15721 ctggagctca ccgttcgcaa ccatccgggc gtaatgaccc acgtttgtgg cctttttgcc
    15781 cgccgcgctt ttaacgttga aggcattctt tgtctgccga ttcaggacag cgacaaaagc
    15841 catatctggc tactggtcaa tgacgaccag cgtctggagc agatgataag ccaaatcgat
    15901 aagctggaag atgttgtgaa agtgcagcgt aatcagtccg atccgacgat gtttaacaag
    15961 atcgcggtgt tttttcagta accgctcaag gcttgaacaa catcgcgctt atcgttaagg
    16021 taagcgcgtt ttttttaccc gccaggacaa gaccatgatc accgttgccc ttatagacga
    16081 tcacctcatc gtccgctccg gctttgcgca gctgctgggg ctggaacctg atttgcaggt
    16141 agttgccgag tttggttcgg ggcgcgaggc gctggcgggg ctgccggggc gcggtgtgca
    16201 ggtgtgtatt tgcgatatct ccatgcccga tatctccggt ctggagctgc taagccagct
    16261 gccgaaaggt atggcgacga ttatgctctc cgttcacgac agtcctgcgc tggttgagca
    16321 ggcgcttaac gcgggggcac gcggctttct ttccaaacgc tgtagcccgg atgaactcat
    16381 tgctgcggtg catacggttg ccacgggcgg ctgttatctg acgccggata ttgccattaa
    16441 actggcatcc ggtcgtcagg acccgctaac caaacgtgaa cgccaggtgg cggaaaaact
    16501 ggcgcaagga atggcggtga aagagattgc cgccgaactg ggcttgtcac cgaaaacggt
    16561 acacgtccat cgcgccaatc tgatggaaaa actgggcgtc agtaacgacg tagagctggc
    16621 gcgccgcatg tttgatggct ggtgatgaag acgttgttct cccgcttaat taccgttatt
    16681 gcctgctttt ttatcttctc tgccgcatgg ttttgcctgt ggagtatcag cctgcatctg
    16741 gttgagcgcc ctgatatggc ggtgctgtta tttccgtttg gtctgcgtct ggggctaatg
    16801 ctgcaatgcc cgcgcggata ctggccggtg ctgctgggcg cggagtggct gctgatttac
    16861 tggctaacgc aggcggtcgg tttaacccat tttccgttat tgatgatcgg tagtttactg
    16921 acgttactgc ccgtagcgct gatctcgcgc tatcgccatc agcgtgactg gcgcaccttg
    16981 ctgttacagg gggcggcgtt aacggcggcg gcgttgttgc agtcgctgcc ctggctttgg
    17041 cacggcaaag agtcgtggaa tgcgctgttg ctgactttaa ctggcggcct gacgctggcc
    17101 ccgatatgtc tggtgttctg gcactatctc gccaataaca cctggctgcc gctcggcccg
    17161 tcactggttt ctcagccaat caactggcgc gggcgacatc tggtctggta cttgctgctg
    17221 tttgttatca gtctctggct ccagttggga ttgccggacg aactgtcgcg ctttacgcca
    17281 ttctgtctgg cgctgccgat tatcgcgctg gcctggcact atggttggca aggggcgctg
    17341 attgcgacgt tgatgaacgc catcgcgctg atcgccagtc aaacctggcg cgatcatccg
    17401 gtggatttat tgctctcgct gctggtgcaa agtctgacag ggttgttgct tggcgctggc
    17461 atccagcggt tgcgtgaact taaccagtcg ctgcaaaagg aactggcgcg caatcagcat
    17521 ctggctgaac ggttgctgga aaccgaagag agcgtgcgcc gtgatgtggc gcgtgagctg
    17581 catgatgata tcggtcagac catcactgct attcgtactc aggcgggcat tgttcagcgg
    17641 ctggcggcag ataacgccag cgtgaagcag agcgggcagc tcatcgaaca actatcgctg
    17701 ggcgtttacg acgcggtgcg ccgtttgttg ggtcggttac gtccgcgcca gttggatgat
    17761 ctcaccctgg agcaggccat ccgctcactg atgcgggaaa tggagctgga agggcgcggt
    17821 attgtcagcc atctcgaatg gcgaatcgat gaatcagcgt taagcgaaaa ccagcgcgtg
    17881 acgctgtttc gtgtctgcca ggaagggctg aacaacattg tgaaacatgc tgatgccagc
    17941 gcggtcaccc tgcaaggctg gcagcaggat gaacggttga tgctggttat tgaagacgat
    18001 ggcagcggtt tgccgccggg ttccgggcaa caaggttttg gcctcaccgg aatgcgcgag
    18061 cgcgtaacgg cgctgggtgg cacattacac atttcctgtc tgcacggcac gcgtgtcagc
    18121 gtttctctac ctcaacgcta tgtctaaggt ttgatgatgt tgccgtttct gaaagcgcct
    18181 gccgatgcgc cattaatgac tgataaatat gaaattgatg cccgctatcg ctactggcgt
    18241 cggcatattc tgctgaccat ctggctgggt tacgcgctgt tttacttcac gcggaaaagt
    18301 tttaacgccg ccgtaccaga aatccttgct aacggcgtgc tcagccgtag cgatatcggc
    18361 ctgttagcga ccctgtttta cattacctat ggcgtgtcga agtttgtctc cggcattgtc
    18421 agcgatcgct caaatgcccg ttattttatg gggatagggc ttatcgccac gggcattatc
    18481 aacattctgt ttggcttctc gacgtcgcta tgggcgtttg ccgtactctg ggtgctgaac
    18541 gcctttttcc agggctgggg ttcaccggtg tgtgcgcgtc tgttaacggc ctggtattca
    18601 cgtaccgagc gcggtggttg gtgggcatta tggaacacgg cgcataacgt cggcggcgca
    18661 ctcattccca ttgtgatggc agcggctgca ctacattacg gctggcgtgc cgggatgatg
    18721 attgctggtt gtatggcgat cgtcgtggga atttttctct gctggcggct gcgcgatcgc
    18781 ccgcaggcgt taggtttacc ggcggtcggt gaatggcgac acgacgcgct ggagatagct
    18841 caacaacaag aaggggcagg gctgacgcgt aaagagatcc tcaccaaata tgtgttgctg
    18901 aatccgtata tctggctgct ttcgttttgc tatgtgctgg tctatgtggt ccgggcggcg
    18961 atcaacgact ggggcaattt gtatatgtcc gagacgctgg gcgtcgatct ggtcacggcg
    19021 aatacggcag tgacgatgtt tgaactgggt ggatttatcg gtgcgctggt agcgggttgg
    19081 ggctcggaca aattgtttaa cggcaaccga gggccgatga atttgatttt cgccgccgga
    19141 attttgcttt cagtcggctc cctgtggctg atgccatttg ccagctacgt gatgcaggca
    19201 acctgcttct tcaccattgg tttttttgtc tttggcccac agatgttaat cggtatggcg
    19261 gcggcagagt gttcccacaa agaggcggca ggggcggcga cggggtttgt cggcttgttt
    19321 gcttatctgg gggcgtcgct tgctggttgg ccgctggcga aagtactcga tacctggcac
    19381 tggagcggat tttttgtggt tatctctatc gccgccggga tttccgcact gctgttactg
    19441 ccctttttga acgcccagac accgcgcgaa gcgtgatgca tctcaccttt tcacttcata
    19501 tccggcaaaa ctaagaaatt ttccaggttt tgcctggacg ctatctcagg cctgatttgc
    19561 tgctgatttt tacaatgcat gcctcacgca ggtattcatt tcaggagtaa cccatgctgg
    19621 ctttcctaaa ccaggttcgc aagccgaccc tggaccttcc gctcgaagtg cggcgcaaaa
    19681 tgtggttcaa accgttcatg caatcctacc tggtggtctt tatcggctac ctgacgatgt
    19741 acctgattcg caagaacttt aacatcgcgc agaacgatat gatttcgacc tacgggttga
    19801 gcatgacgca gctggggatg atcggcctgg gtttctccat cacttatggc gtgggtaaaa
    19861 cgttggtttc atactacgcc gacggcaaaa acaccaaaca attcctgccg ttcatgctga
    19921 tcctgtctgc catttgtatg ctgggcttca gtgccagtat gggcagcggc tcggttagcc
    19981 tgttcctgat gattgccttc tacgccttaa gcggcttttt ccagagtacc ggcggttcgt
    20041 gcagttactc caccatcacc aaatggacgc cgcgtcgtaa acgcgggaca ttcctcggtt
    20101 tctggaatat ttctcacaac cttggcggtg caggcgcagc aggtgtggcg ctgttcgggg
    20161 caaattacct gttcgatggc catgtcatcg gcatgtttat cttcccgtcg attatcgcgc
    20221 tgattgtcgg ttttatcggc ctgcgttacg gcagcgactc cccagaatct tatggcctcg
    20281 gcaaagctga agaactgttc ggcgaggaga tcagcgaaga ggacaaagag acagaatcta
    20341 ccgatatgac caagtggcag atctttgttg agtatgtgct gaaaaacaaa gtgatctggc
    20401 tgctgtgctt cgccaacatt ttcctctatg tggtacgtat tggtatcgac cagtggtcaa
    20461 ccgtatacgc gttccaggaa ctgaaactct ctaaagcggt ggcgattcag ggctttacgc
    20521 tgtttgaagc tggtgcgctg gtcggtacgc tgctgtgggg ctggctctct gacctggcga
    20581 acggtcgccg tggcctggtg gcctgcatcg cgctggcgct gattatcgcc acgctcggtg
    20641 tgtatcaaca tgccagcaac gaatatatct atctggcttc tctctttgcg ttgggtttcc
    20701 tggtctttgg cccgcaattg ttgattggtg tggctgctgt tggctttgta cctaaaaaag
    20761 cgattggcgc tgccgatggt attaaaggca cctttgctta cctgattggt gacagctttg
    20821 ccaagttagg tctgggaatg attgccgatg ggacgccggt attcggcctt accggctggg
    20881 caggcacctt cgccgcactt gatatcgccg cgattggttg tatctgcctg atggcgatag
    20941 tggcggtgat ggaagaacgc aaaatccgcc gcgagaaaaa aattcagcag ttgacagtgg
    21001 cataaacgta actggtgact tttgcccggc atgacgccgg gcttttttat tattccgtga
    21061 cttccagcgt agtgaagaca aacttctcgc catcaaatag cccctgactg gttagtttga
    21121 gcgcggggat cactggcaga gaaagaaacg ccatctgaat aaacggctca tcgggtaacg
    21181 gaccgcattc acgggcggcg gctttcaagg cgtcaatttg ttccgccagc gactgcgccg
    21241 tgtcggtgct catcaggccg gcaatgggca acggcagatg actttggacc tggccgttac
    21301 gtaccacgca cagcccgccg ccatcctgaa tcacctgata gaccgccagc gccatctctt
    21361 cggcactgcg accgatcacc acaatattat ggctgtcatg gctgaccgtc gcagccagcg
    21421 caccttcatt cagtccaaag ccgccaagca aaccacaagc cggagccagc cgttgcccgt
    21481 aacgctcaag tacggcaata aagcacacat catcgcgatc aaaaccattt tcgctgtaga
    21541 cgctggagtg ggagtgcgta atcaattcgt tatggatgac gtcaatgacc cgatagcgtt
    21601 ttccgggcgt aaattgcagg gcaaagtcgc tggcggaaac tggctggcgg gcaatggtgt
    21661 tgccatatgg cggagcggat tgtgccagtc tcgccgactc ttccgcctgt aaggtttgcg
    21721 catcaatcgg ctcgcctttc accagtacct gctgcaccgt gaccttacgc gcatcgctca
    21781 acaggacgat atcggcctgc ttgccgggtg ccagtaagtc gaggtgattc agaccaaagt
    21841 ggcgcgccgt cgaccagctg gcgacgcgat atgccacatg cagcggcaca ttgtgttgtt
    21901 cgatcaggcg gcgaattaag gcatcgatgt gtccttcatg ggcgatctcc cacgggttac
    21961 ggtcatcggt acagagcatg cattgcgggc tgttaaattc gttgatcaac ggtgccagcg
    22021 cgttgagatt gcgggcagcg gacccttcgc ggatcatcaa cgacatgccg agttgtaatt
    22081 tccggcgtcc ttcttccagc tgataacttt cgtggcagtt ttcaataccc gcagtaatat
    22141 aggcgttaag ttctttacca cccaaacccg ggcagtgacc gtccagcgtc aggtggcgaa
    22201 atgcatccag tttatcgagc agcgcattct gcccgctaat tacgccaggg tagtccatca
    22261 tttctgcaag gccggtaacc tgcggatggt cccgccaggc gagcatctgt tcaagggtaa
    22321 aactggcacc gttaacatcg cagccttcca gggcgggtac gcaagagctg acctgtaagt
    22381 actggttttg ccttgcctgt tcggcacagc gggcaaacca ggcgaatccg gcttctccca
    22441 tcacgttgac gatttcatga gggtcgcaaa taacggtcgt caggccgcgc ggcagggtag
    22501 cggtttcaaa ggtgaccggc gtcatcatgc tggattcaat atgcaggtga gcatcaataa
    22561 accctggcac cgccgttgcg ccgcgagcat caatccgctg caaagccgga gcatcagcgt
    22621 attctgcgcc aacaccggca atgtaacgtc ctttaatcac aattgggccg gaaatttctc
    22681 cgccattgat caggtcgaga atagagacat tatcaataat ataatcggca acagcgtcgc
    22741 cacgggaaac ggctaacaat tcctggtatt cagcccggct aatgtgatga aatttatggt
    22801 taatggaatt attcatattt ttctcatttt gattttatca atttgattat tatttatcat
    22861 aaagaaacgt cgcgagatta ttaaagaata tctattaatg tgcaattgaa atgtgataat
    22921 tatcactgaa ccgttgttgt catttgtttt acaaaagcaa gggattaaat ctcctccaat
    22981 ggacaaaaaa atgaataatg acaataccga ttacgtgagt aatgaatcag ggatgctttc
    23041 gcgattattt aaactacctc agcatgggac caccgtccgc acagaattga ttgcggggat
    23101 gaccactttt ttaaccatgg tgtacatcgt ttttgtgaac ccgcaaatcc tcggcgcggc
    23161 acaaatggac ccgaaagtgg tgtttgttac cacctgtttg attgccggta tcggcagtat
    23221 tgcgatgggg atatttgcta acttacccgt ggcgctggct ccggcaatgg ggctgaacgc
    23281 cttctttgcc ttcgtggtcg tgggggcgat gggcatctcc tggcagaccg ggatgggcgc
    23341 aatattctgg ggcgcagttg gactattttt gctcacgctg tttcgtatcc ggtactggat
    23401 gatctccaac a
//
LOCUS       DAZPEB010000045        23030 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZPEB010000045 DAZPEB010000000
VERSION     DAZPEB010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23030
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.046"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..562)
                     /locus_tag="ACHURJ_001576"
     CDS             complement(<1..562)
                     /locus_tag="ACHURJ_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR1280571.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGG"
     gene            complement(781..1758)
                     /gene="epmA"
                     /locus_tag="ACHURJ_001577"
     CDS             complement(781..1758)
                     /gene="epmA"
                     /locus_tag="ACHURJ_001577"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFR1280572.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLNLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            2083..3891
                     /gene="frdA"
                     /locus_tag="ACHURJ_001578"
     CDS             2083..3891
                     /gene="frdA"
                     /locus_tag="ACHURJ_001578"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFR1280573.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRMAGEQATERAATAGNGNEEAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3884..4618
                     /gene="frdB"
                     /locus_tag="ACHURJ_001579"
     CDS             3884..4618
                     /gene="frdB"
                     /locus_tag="ACHURJ_001579"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFR1280574.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4629..5024
                     /gene="frdC"
                     /locus_tag="ACHURJ_001580"
     CDS             4629..5024
                     /gene="frdC"
                     /locus_tag="ACHURJ_001580"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFR1280575.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPDAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            5035..5394
                     /gene="frdD"
                     /locus_tag="ACHURJ_001581"
     CDS             5035..5394
                     /gene="frdD"
                     /locus_tag="ACHURJ_001581"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFR1280576.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            5457..6590
                     /locus_tag="ACHURJ_001582"
     CDS             5457..6590
                     /locus_tag="ACHURJ_001582"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFR1280577.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQMPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6679..7212
                     /gene="blc"
                     /locus_tag="ACHURJ_001583"
     CDS             6679..7212
                     /gene="blc"
                     /locus_tag="ACHURJ_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFR1280578.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDGGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGLFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(7209..7526)
                     /gene="sugE"
                     /locus_tag="ACHURJ_001584"
     CDS             complement(7209..7526)
                     /gene="sugE"
                     /locus_tag="ACHURJ_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFR1280579.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7708..7854)
                     /gene="ecnB"
                     /locus_tag="ACHURJ_001585"
     CDS             complement(7708..7854)
                     /gene="ecnB"
                     /locus_tag="ACHURJ_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFR1280580.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7965..8090)
                     /gene="ecnA"
                     /locus_tag="ACHURJ_001586"
     CDS             complement(7965..8090)
                     /gene="ecnA"
                     /locus_tag="ACHURJ_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFR1280581.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(8142..8708)
                     /gene="efp"
                     /locus_tag="ACHURJ_001587"
     CDS             complement(8142..8708)
                     /gene="efp"
                     /locus_tag="ACHURJ_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFR1280582.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8750..9799
                     /gene="epmB"
                     /locus_tag="ACHURJ_001588"
     CDS             8750..9799
                     /gene="epmB"
                     /locus_tag="ACHURJ_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFR1280583.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEIDETFRQAMAKLRRVGVTLLNQSVLLRDVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQLLIKQGS"
     gene            10173..11042
                     /locus_tag="ACHURJ_001589"
     CDS             10173..11042
                     /locus_tag="ACHURJ_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFR1280584.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEVEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(11245..11598)
                     /locus_tag="ACHURJ_001590"
     CDS             complement(11245..11598)
                     /locus_tag="ACHURJ_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFR1280585.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(11736..13382)
                     /gene="groL"
                     /locus_tag="ACHURJ_001591"
     CDS             complement(11736..13382)
                     /gene="groL"
                     /locus_tag="ACHURJ_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFR1280586.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(13426..13719)
                     /locus_tag="ACHURJ_001592"
     CDS             complement(13426..13719)
                     /locus_tag="ACHURJ_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFR1280587.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            13995..15251
                     /gene="yjeH"
                     /locus_tag="ACHURJ_001593"
     CDS             13995..15251
                     /gene="yjeH"
                     /locus_tag="ACHURJ_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFR1280588.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAAQVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPCAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRSASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEKMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIVLAGLWLLLPKRKTPENGIIT"
     gene            complement(15267..15743)
                     /locus_tag="ACHURJ_001594"
     CDS             complement(15267..15743)
                     /locus_tag="ACHURJ_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFR1280589.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDERDRLDHKDDRQD"
     gene            16080..17516
                     /gene="aspA"
                     /locus_tag="ACHURJ_001595"
     CDS             16080..17516
                     /gene="aspA"
                     /locus_tag="ACHURJ_001595"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFR1280590.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            17634..18935
                     /gene="dcuA"
                     /locus_tag="ACHURJ_001596"
     CDS             17634..18935
                     /gene="dcuA"
                     /locus_tag="ACHURJ_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFR1280591.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            19051..19389
                     /gene="cutA"
                     /locus_tag="ACHURJ_001597"
     CDS             19051..19389
                     /gene="cutA"
                     /locus_tag="ACHURJ_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFR1280592.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            19365..21062
                     /gene="dsbD"
                     /locus_tag="ACHURJ_001598"
     CDS             19365..21062
                     /gene="dsbD"
                     /locus_tag="ACHURJ_001598"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFR1280593.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFTFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNHALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NITANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            21099..21674
                     /locus_tag="ACHURJ_001599"
     CDS             21099..21674
                     /locus_tag="ACHURJ_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1280594.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKQLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            21781..21856
                     /locus_tag="ACHURJ_001600"
     tRNA            21781..21856
                     /locus_tag="ACHURJ_001600"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21814..21816,aa:Phe,seq:gaa)
     gene            22054..>23030
                     /locus_tag="ACHURJ_001601"
     CDS             22054..>23030
                     /locus_tag="ACHURJ_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1280595.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGTGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGS
                     YKGQTVTQLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRKPISENVMNAS"
BASE COUNT         5738 a         5942 c         5700 g         5650 t
ORIGIN      
        1 gcccaccatt taataacaaa atagtaatgc ttactaacag caatactaaa ttcagacaca
       61 ttactgcaat accgcccacc gcagtaatgc gggcaatttt attaatcccc tttgaagcaa
      121 cgacggtgac cagaatcatc catgctactg ccagcagacc aaccacctgc gtaggctcca
      181 gtccggcaat acgccagtgc tgggtcatgt cgctaccata gaggaatgtt gagaacggta
      241 cccaaacttt cgcggaggta ctcaccatcc agatgatata agaggaaaac cacataaacg
      301 taccaatgaa ggcaaaacgt ggtccgacac tattattcat ccaggaatag ataccacctt
      361 cttctttgcg ataagcagct cccatttcag ccatcattaa ggcgaatgga ataaagaata
      421 acaatgcaga aaatatataa aagggaatcg cactataacc cattaagtaa taagccgatg
      481 ggctattggc aaatccaaat acggaagtaa agatcatcaa tatgagtcct atcagactca
      541 tcttttttat cgtgtgaggc atgaaaccat ccttcatttc agcgcgtgta taacacgtgc
      601 ccactgaact gcactgcgca acattgagca ggcagcgtaa aaaccaatac agaattgata
      661 cccggatgat atcaaatatc cagtcttaaa ttgtggcctt taacggaaaa atgaaaacat
      721 atgctacaga atggcgctta tcacgccatt cttcgctgtt aattcagtaa tttttcagaa
      781 ttatgcccgg tcaacgctaa aggcgatgac ttcagccagt gtctccgcgc ccagcgccaa
      841 catcaccaga cgatcaacac ctaatgccac gccggaacag tcaggcatac cgactttcaa
      901 ggcttcaatc agattctggt caatggggtg ctgcggcaaa ccgcgcgccg cgcgcttacg
      961 gttatcttgt tcaaagcgtt gttgctgctc acgggcatcc gttaattcat ggaaaccatt
     1021 cgccagctca atacctttat aataaacctc aaagcgttca gcgacccgat gatcttcggt
     1081 actgatttgc gccagtgatg cctggctggc tggaaagtgg tacacaaagg tcggtttttc
     1141 tttgccaata tttggctcta cgccaaaggt aaacagcaat tgtagcaacg tgtcgcggtc
     1201 ttcttcggta tcagcaacgt tactcaaatt cagtttcgct gcgacttccc gcagttgcgt
     1261 tttgtcggca gagagcgggt caatttccag ataacgcaag aaagcttgtt gataagaaag
     1321 gctttctgct gccgggcagt ccagcacctg ttgtaagaga tcgtccacct cgttcatcaa
     1381 ccggtacata tcatagtgcg gtcgatacca ctcaagcata gtgaactcag ggttgtgata
     1441 acgccccatc tcttcattac ggaagctgcg gcacagctgg aataccggcc cacaaccggc
     1501 aaccagcagg cgtttcatat ggtattccgg gctggtcatt aaccagagat tcatcccctg
     1561 cgaatgcccg gggccaacga aacgtgtctc aaacgggacc aaatgaatat cggttaccgt
     1621 cgcctggctc atacaaggcg tctccacctc cagcactcca cgatcagcaa agaaacgacg
     1681 gatctccgcc ataatcgccg cgcgttttaa taagttagga atggatgcgc tcggctgcca
     1741 ggatgccgtt tcgctcatag ttaaatctcc agtttttgac aagggcacga agtctactcg
     1801 caacgcgacg gcgagacaaa ttttacgcag gaatcaaaca gcggtgggca gtgactaaaa
     1861 aaagcacgat ctgatggttt agtaattaaa ttaatcatct tcagtgataa tttagccctc
     1921 ttgcgcacta aaaaaatcga tctcgtcaaa tttcagactt atccatcaga ctatactgtt
     1981 gtacctataa aggagcagtg gaatagcgtt cgcagaccgt aactttcagg cacttaccct
     2041 gaagtacggg gctgtgggat aaaaacaatc tggaggaatg tcgtgcaaac ctttcaagcc
     2101 gatcttgcca ttgtaggcgc cggtggcgcg ggattacgtg ctgcaattgc tgccgcgcag
     2161 gcaaatccaa atgcaaaaat cgcactaatc tcaaaagtat acccgatgcg tagccatacc
     2221 gttgctgcag aagggggctc cgccgctgtc gcgcaggatc atgacagctt cgaatatcac
     2281 tttcacgata cagtagcggg tggcgactgg ttgtgtgagc aggatgtcgt ggattatttc
     2341 gtccaccact gcccaaccga aatgacccaa ctggaactgt gggggtgccc atggagccgt
     2401 cgcccggatg gtagcgtcaa cgtacgtcgc ttcggcggca tgaaaatcga gcgcacctgg
     2461 ttcgccgccg ataagaccgg cttccatatg ctgcacacgc tgttccagac ctctctgcaa
     2521 ttcccgcaga tccagcgttt tgacgaacat ttcgtgctgg atattctggt tgatgatggt
     2581 catgttcgcg gcctggtagc aatgaacatg atggaaggca cgctggtgca gatccgtgct
     2641 aacgcggtcg ttatggctac tggcggtgcg ggtcgcgttt atcgttacaa caccaacggc
     2701 ggcatcgtta ccggtgacgg tatgggtatg gcgctaagcc acggcgttcc gctgcgtgac
     2761 atggaattcg ttcagtatca cccaaccggt ctgccaggtt ccggtatcct gatgaccgaa
     2821 ggctgccgtg gtgaaggcgg tattctggtc aacaaaaatg gctaccgtta tctgcaagat
     2881 tacggcatgg gcccggaaac tccgctgggc gagccgaaaa acaaatatat ggaactgggt
     2941 ccacgcgaca aagtttctca ggccttctgg cacgaatggc gtaaaggcaa caccatctcc
     3001 acgccgcgtg gcgatgtggt ttatctcgac ttgcgtcacc tcggcgagaa aaaactgcat
     3061 gaacgtctgc cgttcatctg cgaactggcg aaagcgtacg ttggcgtcga tccggttaaa
     3121 gaaccgattc cggtacgtcc gaccgcacac tacaccatgg gcggtatcga aaccgatcag
     3181 aactgtgaaa cccgcattaa aggtctgttc gccgtgggtg aatgttcctc tgttggtctg
     3241 cacggtgcaa accgtctggg ctccaactcc ctggcggaac tggtggtctt cggccgtatg
     3301 gccggtgaac aagcgacaga gcgtgcagca actgccggta atggcaacga agaggcaatt
     3361 gaagcgcagg cagctggcgt tgaacaacgt ctgaaagatc tggttaacca ggatggcggc
     3421 gaaaactggg cgaagatccg cgacgaaatg ggcctagcaa tggaagaagg ttgcggtatc
     3481 taccgtacgc cggaactgat gcagaaaacc atcgacaagc tggcagagct gcaggaacgc
     3541 ttcaagcgcg tgcgcatcac cgacacctcc agcgtgttca acaccgacct gctctacacc
     3601 attgaactgg gccacggtct gaacgttgct gaatgtatgg cgcactccgc aatggcacgt
     3661 aaagagtccc gcggcgcaca ccagcgtctg gacgaaggtt gcaccgagcg tgacgacgtc
     3721 aacttcctca aacacaccct cgccttccgc gatgctgatg gcacgactcg cctggagtac
     3781 agcgacgtga agattactac gctgccgcca gctaaacgcg tttacggtgg cgaagcggat
     3841 gcagccgata aggcggaagc agccaataag aaggagaagg cgaatggctg agatgaaaaa
     3901 cctgaaaatt gaggtggtgc gctataaccc ggaagtcgat accgcaccgc atagcgcatt
     3961 ctatgaagtg ccttatgacg caactacctc attactggat gcgctgggct acatcaaaga
     4021 caacctggca ccggacctga gttaccgctg gtcctgccgt atggcgattt gtggttcctg
     4081 cggcatgatg gttaacaacg tgccaaaact ggcatgtaaa accttcctgc gtgattacac
     4141 cgacggtatg aaggttgaag cgttagctaa cttcccgatt gaacgcgatc tggtggtcga
     4201 tatgacccac ttcatcgaaa gtctggaagc gatcaaaccg tacatcatcg gcaactcccg
     4261 caccgcggat cagggtacta acatccagac cccggcgcag atggcaaagt atcaccagtt
     4321 ctccggttgc atcaactgtg gtctgtgcta cgccgcgtgc ccgcagtttg gcctgaaccc
     4381 agagttcatc ggtccggctg ccattacgct ggcgcatcgt tataacgaag atagccgcga
     4441 ccacggtaag aaggagcgta tggcgcagtt gaacagccag aacggcgtat ggagctgtac
     4501 tttcgtgggc tattgctccg aagtctgccc gaaacacgtc gatccggctg cggccattca
     4561 gcagggcaaa gtagaaagtt cgaaagactt tcttatcgcg accctgaaac cacgctaagg
     4621 agtgcaacat gacgactaaa cgtaaaccgt atgtacggcc aatgacgtcc acctggtgga
     4681 aaaaattgcc gttttatcgc ttttacatgc tgcgcgaagg cacggcggtt ccggctgtgt
     4741 ggttcagcat tgaactgatt ttcgggctgt ttgccctgaa aaatggcccg gacgcctggg
     4801 cgggattcgt cgacttttta caaaacccgg ttatcgtgat cattaacctg atcactctgg
     4861 cggcagctct gctgcacacc aaaacctggt ttgaactggc accgaaagcg gccaatatca
     4921 ttgtaaaaga cgaaaaaatg ggaccagagc caattatcaa aagtctctgg gcggtaactg
     4981 tggttgccac catcgtaatc ctgtttgttg ccctgtactg gtaaggagcc tgagatgatt
     5041 aatccaaatc caaagcgttc tgacgaaccg gtattctggg gcctcttcgg ggccggtggt
     5101 atgtggagcg caattattgc gccggtgatg atcctgctgg tgggtattct gctgccactg
     5161 gggctgtttc cgggtgatgc gctgagctac gagcgcgttc tggcgttcgc gcagagcttc
     5221 attggtcgcg tattcctgtt cctgatgatc gttctgccgc tgtggtgtgg tttacaccgt
     5281 atgcaccacg cgatgcacga tctgaaaatt cacgtgcctg cgggcaaatg ggttttctac
     5341 ggtctggctg ctatcctgac agttgtcacg ctgattggta tcgttacaat ctaacgtatc
     5401 gccaatgtaa atccggcccg cctatggcgg gccgttttgt atggaaacca gaccttatgt
     5461 tcaaaacgac gctctgcgcc ttattaatta ccgcctcttg ctccacattt gctgcccctc
     5521 aacaaatcaa cgatattgtg catcgcacaa ttaccccgct tatagagcaa caaaagatcc
     5581 ccggtatggc ggtggcggta atttatcagg gtaaacctta ttactttacc tggggctatg
     5641 cggacatcgc caaaaagcag cccgtcacac agcaaacgtt gtttgagtta ggttcggtca
     5701 gcaaaacatt tacgggcgtg cttggtggcg atgctattgc tcgaggggaa atcaagttaa
     5761 gcgatcccgc aacaaaatac tggcctgaac ttaccgctaa acagtggaat gggatcacac
     5821 tattacatct tgcgacctac accgctggcg gcctgccatt gcagatgccg gatgaagtga
     5881 aatcctcaag cgacttgctg cgcttctatc aaaactggca gcctgcatgg gctccaggaa
     5941 cacaacgtct gtatgccaac tccagtatcg gtttgttcgg cgcactggct gtgaagccgt
     6001 ctggtttgag ttttgagcag gcgatgcaaa ctcgtgtctt ccagccactc aaactcaacc
     6061 atacgtggat taatgtaccg cccgcagaag aaaagaatta cgcctgggga tatcgcgaag
     6121 gcaaggcagt tcatgtttcg ccaggggcgt tagatgctga agcttatggt gtgaagtcga
     6181 ccattgaaga tatggcccgc tgggtgcaaa gcaatttaac cccccttgat atcaatgaga
     6241 aaacgcttca acaagggata caactggcac aatctcgcta ctggcaaacc ggcgatatgt
     6301 atcagggcct gggctgggaa atgctggact ggccggtcaa tcctgacagc atcattaacg
     6361 gcagtgacaa taaaattgca ctggcagcac gcccggtaaa agcgattaag cccccaactc
     6421 ctgcagtacg cgcatcatgg gtacataaaa cgggggcgac cggcggattt ggtagctatg
     6481 tcgcgtttat tccagaaaaa gagctgggta tcgtgatgct ggctaacaaa aactatccca
     6541 atccagcgag agtcgccgcc gcctgtcaga ttctcaacgc tctacagtaa aatttcatcg
     6601 ggtccgaatt ttcggacctt ttctgcgctt ttccttgctg tcatctacac ttagaaaaaa
     6661 accagtaagg aaacatttat gcgcctgctc cctctcgttg ccgcagcgac agctgcattt
     6721 ctggtcgttg cctgcagttc tcctacgccg ccgcgtggcg tgacagtagt gaataatttc
     6781 gacgctaaac gctatctcgg tacctggtat gagattgccc gttttgatca ccgttttgaa
     6841 cgtggactgg aaaaagtcac cgcaacatac agtctgcgtg atggcggcgg cctgaatgtc
     6901 atcaataaag gctataaccc tgacagagaa atgtggcagc agagtgaagg gaaagcgtac
     6961 tttaccggcg caccaacacg cgctgcgctg aaagtgtcat tctttggtct tttctatggc
     7021 ggttataacg tcattgcact tgatcgggaa taccgccatg cgctggtttg cggcccggac
     7081 cgcgactacc tgtggatact ctcccgcacg ccaaccattt ctgacgaagt gaaacaggag
     7141 atgctggcag tcgcgacccg ggaagggttt gatgtcagta agtttatttg ggtacagcag
     7201 cccggtagtt agtgagtgct gagtttcaga ccaataatcc ccaatacgat taacgccaga
     7261 ctcgccaggc gcatcgggtt agcggactca ccgagcagca caatgccggt gatagccgcg
     7321 ccgactgcgc caatacccgt ccacacggca taagccgtcc ctactggtaa cgatttcatc
     7381 gcccaggcaa gtagcgccat actgacaatc atcgccgtca cggtaataac actcggcgtc
     7441 aaacgactaa agccgtgggt atatttcagg ccaacggccc ataccacttc cagcagacca
     7501 gcaataacta agataatcca ggacatatca ggctccggag aattggggcc gtccccggtg
     7561 aaaagacgcg tttgcaggtc gtcctgcaaa gctgatggtt gaacggatat tttgccaggt
     7621 ggcaggggaa tttcaactat taccttagtg actatcgcag aaaaaacaaa cggcacgaca
     7681 cagctgtcgt accgttattg cttattgcta ttgctgcgct ttcgttgctg cgccagaaat
     7741 cgcgttaccg ccatcagaaa tgtcttcacc aacgccacgc gtggtgttgc aggcagttaa
     7801 tactgttgaa agcaccagaa cagaaaagat cgctgcaatt gtcttcttca ccataacgtc
     7861 ttccttttag gtaatgttat ttatgtttgc ctatagcaaa ttaataatag acaacatcgc
     7921 caaaaatgac ggaaatctga tgatttttcg gaagagaaga aaaattagct ggcagcgcga
     7981 gagatggagt tgccgagatg tttgatgtct tcgccgaaac cgcgagcggt gttacagccc
     8041 gtgagcagcg tactggcaag caaaacaaga acgataaggc gtttcatcat ctctgacctg
     8101 aataagtgat ggtgcagcct gcaggccgca ccacaaccgc attacttcac gcgagagacg
     8161 tattcaccag agcgggtatc cactttgatg acttcgccga tttgtacgaa cagcggaacc
     8221 ttaaccacag cgccagtaga cagggtagcc ggtttgccgc cagtacctgc ggtatcacct
     8281 ttcaggcccg gatcggtatc aacgatttcc agttcaacga agttcggcgg agtaacggag
     8341 atcggctgac cattccacag agttacgata cactctgcct gatccagcag ccatttagcg
     8401 ttatcgccaa ttgcttttgc atcagcagac agctgctcga aagtttcgtt gttcatgaag
     8461 tgccagaact caccgtcgtt gtacaggtaa gtcaggttca tatcgacaac atcagcgcct
     8521 tcagcggaat cagtagattt gaaggttttt tctacgcgag taccggtcag cagacgacgc
     8581 agtttaacgc gagcaaatgc ctggccttta cccggtttta cgaattcact cgcttcaacc
     8641 gcgtaaggtt cgccgtctaa catgatttta agaccagcac gaaaatcgtt gctatagtac
     8701 gttgccataa ggccctctga aatttgttaa ttggtagcta agccacaaaa tggcgcatat
     8761 tgtaacccta aataccccat ccagagaaga ttggttaacg caacttgccg atgttgtgac
     8821 cgatcctgat gaacttctgc gtcttttgaa tatagacgcg gacgaaaaac tgttggccgg
     8881 acgcagcgcc aaaaagctgt ttgccctgcg tgtgccccgc tcatttatcg atcgcatgga
     8941 gaaaggcaat ccggacgatc ctcttttgcg tcaggtactt acctcgcaag atgagtttgt
     9001 cgtcgcgtcc ggattctcca ccgacccact ggaagaacag cacagcgtag tgcctggttt
     9061 gttgcataaa taccacaacc gggcgctttt gctggtcaaa ggcggctgcg cggtaaattg
     9121 ccgctattgc ttccgtcgcc acttccccta tgccgaaaat cagggcaaca agcgtaactg
     9181 gcagactgca cttgagtatg ttgctgcgca tccggaactg gacgagatga ttttctccgg
     9241 cggcgatccg ctgatggcga aagatcacga gctggactgg ttgctcacac aactggaagc
     9301 catcccgcat atcaaacgtc tgcggattca cagtcgtctg ccgattgtga tcccggcacg
     9361 catcacagat gcgctggttg aacgcttttc acattctacg ctgcaaatcc tgctggtgaa
     9421 tcacatcaac catgccaatg agatagatga aacattccgt caggcgatgg ctaagttgcg
     9481 ccgggtaggc gttactttgc tgaaccagag cgttctgtta cgtgatgtga acgataacgc
     9541 acaaacgctg gcaaacctga gtaatgcgtt gttcgatgcc ggcgtgatgc cgtattacct
     9601 gcatgtgctc gataaagtac agggcgcagc gcattttatg gtgagtgatg acgaagcacg
     9661 gcagattatg cgtgagttgc tgacattggt gtcgggatat ctggtgccga aactggcgcg
     9721 agaaattggc ggcgaaccca gcaaaacgcc gctggatctc cagctacgcc agcagttact
     9781 gattaaacag ggaagttaaa cattaaaatt gcctgatgcg ctacgcttat caggcctaca
     9841 tcgtgcattg aaattgaatg gatttgcaat gaactgtagg ccggataagg cgtttacgcc
     9901 gcatccggca agagcaacaa acgcaagtac agcaacctgt cattaaactg agaaatttaa
     9961 tctttgcccg ttgcataacc tttaattatt catatgcatt gctgttgtta ctttttatat
    10021 agctaacacc aataattaac ctttcttttc tgtttatata gagtgagcac ggctatttat
    10081 gttattttaa caactgttac taccaagccc atttatctgg cttataaatt aaaattaatt
    10141 atttaattga gaaatggtta gggagaacct acatggccat aagcatcaag ggtgttaata
    10201 ccggtgttat tcgcaagagt aataatttca ttgcactggc gctgaaaatt aaagaacctc
    10261 gcaataaaga atcgttgttc tttatgtctg tcatggaact gcgcgacctg ctgattgcgc
    10321 tggaaagtcg tctgcatcaa aagcataaac tggatgctgc tgcacgcctt cagtatgagc
    10381 aagctcgcga taaagtcatt aagaaaatgg cagaaaacat cccagaaatt ctggttgatg
    10441 agcttaaaaa tgccgatatt aatcgtcggg ttaacacgct ggaacttacc gataatcaag
    10501 gcgaaaatct taccttcgta ttaacgctac atgacggtag caaatgtgag ttagtcgtca
    10561 atgagttgca gattgaaatg ctagcgcgag caatcattca tgcaattaat aatgcggaaa
    10621 tgcgtgaact ggcattacgt attacctcac tgttagattt cctgccgtta tatgacgttg
    10681 actgccagga aaatggcaat ctggaatatg acacctattc ccaaccggaa tggaaacata
    10741 acttgttcga ccattatctg gcggtgcttt accgctttaa agatgaaagt ggcaaagaac
    10801 aatttagcgg cgcagtggtg aaaacgcgtg aagcaacgcc gggtaaagaa gtagaggcga
    10861 tcactcgccg aatgcttgat ttcagcccac gactgaaaaa actcgcgggt gtgccatgtc
    10921 aggtctatgt tcgcaccgta gcggcgaata acgcccaacc gctaacccag gatcaatgct
    10981 tacgcgcgct gcatcactta cgtgttcaat ccaccagcaa aactgcacca caggcgaaat
    11041 aatctcatct ttgctaatgc gaggctggag ccatccagcc tgaaatgact gataacgtga
    11101 tcgttggtca tgccggatgc ggcgtaaacg ccttatccgg cctacaaatt ctcaagcatt
    11161 caatcagttg caatgcctgc tgtaggcctg ataagcgaag cgcatcaggc aataatacgt
    11221 ttgtcaccca gcctctggat cacatcagtt cgggcactta taaacctgac cgataatctg
    11281 gctatccgtc gggacaaaac tggacaacat tccctgcgac gggctgctga tgccataaat
    11341 cacgttaccg cccattgcag ccgcctggtt gcgcagatcg tttgctgcgc cgcgcataga
    11401 accgccctct tctccgtgtt gcccggaaag ccagttgctt tgcttacctg tcgcagtacc
    11461 aatcagctgg cactctgcgc ctggctgctc gtccacaatg cgtacactct gaccggcagc
    11521 actcaattcg ttgctggagc tacaacccgc catcagtagc gcggcaccga caatccctgc
    11581 taagtatttt acgtgcatgt tattccccat aatcaatgag ctggacgcca ctcgcgtcgt
    11641 ccgtgtctga atcttatact agaaagatga ccaaaagaaa aacccccaga catttctgcc
    11701 cgggggtttg tttatttctg cgaggtgcag ggcaattaca tcatgccgcc catgccaccc
    11761 atgccgccca taccgccagc agcgcctaag tcagctgcat cgtttttcgg caggtcggta
    11821 accatgcact cggtggtgat catcaggcca gccacagaag ccgcgtactg cagagcagaa
    11881 cgggttactt tggttggatc caagataccc atgtcgatca tgttgccgta ttcttcggtt
    11941 gctgcgttgt aaccgtagtt gccgtcgccg cctttaacag tgttagcaac aacagacggt
    12001 tcttcgccgc agttcaatac gatctgacgc agcggagctt ccattgcacg cagtgcaact
    12061 ttgataccca cgttctggtc ttcgttctga ccacgcaggt cagccagttt agacgctacg
    12121 cggatcagcg caacaccacc accagcaacc acgccttctt ctaccgcagc acgggtcgcg
    12181 tgcagggcat cttcaacgcg tgcttttttc tctttcattt caacttcggt agcagcacct
    12241 actttgataa ctgcaacgcc gcctgccagt ttcgctacgc gctcctgcag tttttcacgg
    12301 tcgtagtcag aagttgcttc ttcaatctgc tgacggatct gagcaacacg gccctggatt
    12361 gcagcttctt cacccacgcc atcaatgatg gtggtggtgt ctttgttgat cacaacacgt
    12421 ttagcctgac ccaggtcttc cagggttgct ttttccagct ccataccgat ctcttcagag
    12481 attacggtac cgccagtcag ggttgcgata tcctgcagca tagctttacg gcgatcgccg
    12541 aagcccggag ctttaaccgc agcaactttc acgatgccac gcatggtgtt aacaaccaga
    12601 gttgccagcg cttcgccttc aacatcttca gcgatgatca gcagcggttt acctgcttta
    12661 gcaacggctt ccagaaccgg cagcatttcg cggatgttgg agattttctt gtcagccagc
    12721 aggatgaacg ggctttccag ttctactgcg ccagtttccg gcttgttgat gaagtaagga
    12781 gacaggtagc cacggtcgaa ctgcatacct tcaaccacgt ccagttcgtc ctgcagaccg
    12841 gtaccgtctt caacggtgat aacgccttct ttaccgactt tgtccatcgc ttcagcgatc
    12901 agtttaccta cggtttcgtc ggagttagcg gagatggtac caacctgagc aatcgcttta
    12961 gagtcagagc atggtacgga cagcgctttc agttcttcaa ctgcagcggt aacagctttg
    13021 tcgataccac gtttcaggtc catcgggttc atgcccgcag caactgcttt cagaccctca
    13081 gtgatgatag cctgagccag tacggttgca gtggtggtac cgtcgcctgc agcgtcgttc
    13141 gctttagagg caacttcttt caccatctgc gcacccatat tttcgaactt gtcttccagt
    13201 tcgatttcac gagcaacgga aacaccatct ttggtgatgg tcggtgcacc gaaagattta
    13261 tccagaacta cgttacggcc tttcggaccg agggtaactt tcactgcatc tgccagtacg
    13321 tttacgccgc gcagcatttt cacacgagcg tcgttaccga attttacgtc tttagctgcc
    13381 attatcttta ttccttaaat tcgtatgttc agtgtcgtgc gaggattacg cttcaacaat
    13441 tgccagaatg tcgctttcgg acatgatcaa cacttcttca ttgtcgatct tctcagattt
    13501 cacaccgtag ccatcgttga aaataacgat gtcgccaact ttcacatcca gcggcttcac
    13561 ttcgccattt tcaaggatac ggccattgcc gacagccagc acttcgccgc gggtggattt
    13621 agccgctgca gagccggtca gaacgatgcc gccagcagat ttagtttcaa cttctttacg
    13681 cttgacgatc acgcgatcat gcaatggacg aatattcatt gataactctc ctttgagaaa
    13741 gtccgtatct gttatgggtg acgccggagc ttacgtggtt tcccggctgg tgaccagaga
    13801 aatggggatg aggattcgcc ccttcaaggg ggaaaaagaa aaaaaattct gatcagcaca
    13861 aaatcgggtg aaaaccctga ttcacctcac atttcatcgc aatttcttca tcgccgtcat
    13921 aagcgaatct gattgtgcta ccatcgaaaa tctacgcagt tgcccaaaat ttgggcgcaa
    13981 tcggatatca acccatgagt ggactcaaac aagaactggg gctggcccag ggcattggcc
    14041 tgctatcgac gtcattatta ggcaccggcg tgtttgccgt tcctgcgtta gctgcacagg
    14101 tagcaggcaa taacagcctg tgggcgtggc ccgttttgat tatcttagtg ttcccgattg
    14161 cgattgtgtt tgcgattctg ggtcgccact atccctgcgc aggcggtgtc gcgcacttcg
    14221 tcggtatggc gtttggttcg cggcttgagc gagtcaccgg ctggttgttt ttatcggtca
    14281 ttcccgtggg tttgccagcc gcgctacaaa ttgccgccgg gttcggccag gcaatgtttg
    14341 gctggcatag ctggcaactg ttgttggcag aactcggtac gctggcgctg gtgtggtata
    14401 tcggtactcg cagtgccagt tccagtgcta atctacaaac cgttattgcc ggacttatcg
    14461 tcgcgctgat tgtcgctatc tggtgggcgg gcgatatcaa acctgcgaat atccccttcc
    14521 cggcacctgg taatatcgaa cttaccgggt tatttgctgc gttatcagtg atgttctggt
    14581 gttttgtcgg tctggaagca tttgcccatc ttgcctcgga atttaaaaat ccagagcgtg
    14641 attttcctcg cgcattgatg attggtctgc tgctggcagg attagtctac tggggctgta
    14701 cggtagtcgt cttacacttc gacgcctatg gtgaaaaaat ggcggcggca gcatcgcttc
    14761 caaaaattgt agtgcaactg ttcggtgtag gagcgttatg gattgcctgc gtgattggct
    14821 atctggcctg ctttgccagt ctcaacattt atatacagag cttcgcccgc ctggtctggt
    14881 cgcaggcgca acataatcct gaccactacc tggcacgcct ctcttctcgc catatcccga
    14941 ataatgccct caatgcggtg ctcggctgct gtgtggtgag cactttggtg attcatgctt
    15001 tagagatcaa tctggacgct cttattattt atgccaatgg catctttatt atgatttatc
    15061 tgttatgcat gctggcaggc tgtaaattat tgcaaggacg ttatcgacta ctggcggtgg
    15121 ttggcgggct gttatgcgtt ctgttactgg caatggtcgg ctggaaaagt ctctatgcgc
    15181 tgatcgtgct ggcggggtta tggctgttgc tgccaaaacg aaaaacgccg gaaaatggca
    15241 taatcacata atccggcgtt tcgacattaa tcctggcgat cgtctttatg atcaaggcgg
    15301 tcgcgctcat catcctttcg ctggtactca ccatcaaaag tattaccgcc accggtcccg
    15361 gcgctaaaac cgccgcctgg catacgagaa aagcgcaaat gcggcatcaa cttcactgtc
    15421 agatgctttt gcaccggcgg taataaaagc agaagaccga ggaagtcggt gaaaaagccc
    15481 ggtaataaaa gcagcaaacc ggcaatgatc agcgaaacac ttttaatcat ctccgccgct
    15541 gggttttcac ccgccgccat tttttgctgc atcagcacga aattcttaaa gccctggtta
    15601 cgtaccagtg acatcccgat aactgacgtg aatataacca gcacgagggt cagcaatacc
    15661 cccaatacat gggcaacctg aataaagatt gaaatctcaa tatagacata aaggaaaatg
    15721 gcaataaaag gtaaccagcg caaaggtttc tcctgtaata gcagccggta aaacccggct
    15781 acctgaatgg gttgcgaatc gcgttcagct tatattgtgg tcattagcaa aatttcaaga
    15841 tgtttgcgca actatttttg gtagtaatcc caaagcggtg atctatttca caaattaata
    15901 attaaggggt aaaaaccgac acttaaagtg atccagatta cggtagaaat cctcaagcag
    15961 catatgatct cgggtattcg gtcgatgcag gggataatcg tcggtcgaaa aacattcgaa
    16021 accacatata ttctgtgtgt ttaaagcaaa tcattggcag cttgaaaaag aaggttcaca
    16081 tgtcaaacaa cattcgtatc gaagaagatc tgttgggtac cagggaagtt ccagctgatg
    16141 cctactatgg tgttcacact ctgagagcga ttgaaaactt ctatatcagc aacaacaaaa
    16201 tcagtgatat tcctgaattt gttcgcggta tggtaatggt taaaaaagcc gcagctatgg
    16261 caaacaaaga gctgcaaacc attcctaaaa gtgtagcgaa tgccatcatt gccgcatgtg
    16321 atgaagtcct gaacaacgga aaatgcatgg atcagttccc ggtagacgtc taccagggcg
    16381 gcgcaggtac ttccgtaaac atgaacacca acgaagtgct ggccaatatc ggtctggaac
    16441 tgatgggtca ccagaaaggt gaatatcagt acctgaaccc gaacgaccat gttaacaaat
    16501 gtcagtccac taacgacgcc tacccgaccg gtttccgtat cgcagtttac tcttccctga
    16561 ttaagctggt agatgcgatt aaccaactgc gtgaaggctt tgaacgtaaa gctgtcgaat
    16621 tccaggacat cctgaaaatg ggtcgtaccc agctgcagga cgcagtaccg atgaccctcg
    16681 gtcaggaatt ccgcgctttc agcatcctgc tgaaagaaga agtgaaaaat atccaacgta
    16741 ccgctgaact gctgctggaa gttaaccttg gcgcaacagc aatcggtact ggtctgaaca
    16801 cgccgaaaga gtactctccg ctggcagtga aaaaactggc tgaagtcact ggcttcccat
    16861 gcgtaccggc tgaagacctg atcgaagcga cctctgactg cggcgcttat gttatggttc
    16921 acggcgcgct gaaacgcctg gctgtgaaga tgtccaaaat ctgtaacgac ctgcgcttgc
    16981 tctcttctgg cccacgtgcc ggcctgaacg agatcaacct gccggaactg caggcgggct
    17041 cttccatcat gccagctaaa gtaaacccgg ttgttccgga agtggttaac caggtatgct
    17101 tcaaagtcat cggtaacgac accactgtta ccatggcagc agaagcaggt cagctgcagt
    17161 tgaacgttat ggagccggtc attggccagg ccatgttcga atccgttcac attctgacca
    17221 acgcttgcta caacctgctg gaaaaatgca ttaacggcat cactgctaac aaagaagtgt
    17281 gcgaaggtta cgtttacaac tctatcggta tcgttactta cctgaacccg ttcatcggtc
    17341 accacaacgg tgacatcgtg ggtaaaatct gtgccgaaac cggtaagagt gtacgtgaag
    17401 tcgttctgga acgcggtctg ttgactgaag cggaacttga cgatattttc tccgtacaga
    17461 atctgatgca cccggcttac aaagcaaaac gctatactga tgaaagcgaa cagtaatcgt
    17521 acagggtagt acaaataaag aaggcacgtc agatgacgtg ccttttttct tgtgaacagt
    17581 aacttaaaaa caacaaccta atatcaactt gttaaaaaac aaggaaggct aatatgctag
    17641 ttgtagaact catcatagtt ttgctggcga tcttcttggg cgccagattg gggggaatag
    17701 gtattggttt tgcaggcgga ttgggggtgc tggttcttgc cgctattggc gttaaacccg
    17761 gtaacatccc gttcgatgtc atttccatta tcatggcggt tatcgccgct atttctgcca
    17821 tgcaggttgc tggcggtctg gactatctgg ttcatcagac agaaaagctg ctgcgccgta
    17881 acccgaaata catcacgatc ctcgcaccta tcgtgaccta tttcctgaca atctttgctg
    17941 gtactggcaa catctctctg gcgacactgc cagttatcgc tgaagttgcg aaggaacaag
    18001 gcgttaaacc ttgccgtccg ctctctactg cagtggtatc cgcgcagatt gcgatcaccg
    18061 catcgccaat ctcagcggca gtggtttaca tgtcttccgt gatggaaggt catggcatca
    18121 gctacctcca tctgctctcc gtggtcatcc cgtccaccct gctggcggtt ctggtgatgt
    18181 cctttctggt cactatgctg ttcaactcca aactctctga cgatccgatt tatcgcaagc
    18241 gtctggaaga gggcctggtt gaactgcgcg gtgaaaagca gattgaaatc aaatccggtg
    18301 caaaaacgtc cgtctggctg ttcctgctgg gcgtagttgg cgtggttatc tatgcaatca
    18361 tcaacagccc aagcatgggt ctggttgaaa aaccgctgat gaacaccacc aacgcaatcc
    18421 tgatcatcat gctcagcgtt gcaactctga ccaccgttat ctgtaaagtc gataccgaca
    18481 acatcctcaa ctccagcacc ttcaaagcag gtatgagcgc ctgtatttgt atcctgggtg
    18541 ttgcgtggct gggcgatact ttcgtttcca acaacatcga ctggatcaaa gataccgctg
    18601 gtgaagtgat tcagggtcat ccgtggctgc tggccgtcat cttcttcttt gcttctgctc
    18661 tgctgtactc tcaggctgca accgcaaaag cactgatgcc gatggctctg gcactgaacg
    18721 tttcaccgct gaccgctgtt gcttctttcg ctgcggtgtc tggtctgttc attctgccga
    18781 cctacccgac gctggttgct gcggtacaga tggatgacac gggtactacc cgtatcggta
    18841 aattcgtctt caaccatccg ttcttcatcc cgggtactct gggtgttgcc ctggccgttt
    18901 gcttcggctt cgtgctgggt agcttcatgc tgtaatgatc aatcgcgggg cgttcacgcc
    18961 ccgctttctt tcccgccgac taacatcctt cccccgtacg ttgtatagtg acctctctct
    19021 tgcggttcca tctgttcttg cgaggtgttt atgcttgatg aaaaaagttc gaataccgcg
    19081 tctgtcgtgg tgctatgtac ggcaccagat gaagcgacag cccaggattt agctgccaaa
    19141 gtgctggcgg aaaaactggc ggcctgcgcg accttgatcc ccggcgctac ctctctctat
    19201 tactgggaag gtaagctgga gcaagaatac gaagtgcaga tgattttaaa aactaccgta
    19261 tctcaccagc aggcactgct ggaatgcctg aagtctcatc atccatatca aaccccggaa
    19321 cttctggttt tacctgttac acacggagac acagattacc tctcatggct caacgcatct
    19381 ttacgctgat cctgctactt tgcagcactt ccgtttttgc cggattattc gacgcgccgg
    19441 gacgttcaca atttgtcccc gcggatcaag cctttacttt tgattttcag caaaaccaac
    19501 atgaccttaa tctgacctgg cagatcaaag acggttacta cctctaccgt aaacagatcc
    19561 gcattacgcc ggaacacgcg aaaattgccg acgtgcagct gccgcaaggc gtctggcatg
    19621 aagatgagtt ttacggcaaa agcgagattt accgcgatcg gctgacgctt cccgtaacca
    19681 tcaaccaggc gagtgcggga gcgacgttaa ctgtcaccta ccagggctgt gctgatgccg
    19741 gtttctgtta tccgccagaa accaaaaccg ttccgttaag cgaagtggtc gccaacaacg
    19801 cagcgtcaca gcctgtgtct gtttcgcagc aagagcagca caccgcgcaa ttgccctttt
    19861 ccgcgctctg ggcgttgttg atcggtattg gtatcgcctt tacgccatgc gtgctgccaa
    19921 tgtacccact gatttctggc atcgtgctgg gtggtaaaca gcggctctcc actgccagag
    19981 cattgttgct gacctttatt tatgtgcagg ggatggcact gacttacacg gcgctgggtc
    20041 tggtagttgc cgccgcaggg ttacagttcc aggcggcgct acagcaccca tacgtgctca
    20101 ttggcctcgc catcgtcttt accttgctgg cgatgtcaat gtttggcttg tttactctac
    20161 aactcccctc ttcgctgcaa acacgtctca cgttgatgag caatcgccaa cagggcggct
    20221 cacctggcgg tgtgtttgtt atgggggcga ttgccggact tatctgttca ccatgcacca
    20281 ccgcaccgct tagcgcgatt ctgctgtata tcgcccaaag cgggaacatg tggctgggcg
    20341 gcggcacgct ttatctctat gcgttgggca tgggcctgcc gctgatgcta attaccgtct
    20401 ttggtaaccg cttgctgccg aaaagcggcc cgtggatgga acaagtcaaa accgcgtttg
    20461 gttttgtgat cctcgcactg ccggtcttcc tgctggagcg agtgattggt gatgtatggg
    20521 gattacgctt gtggtcggcg ctgggtgtcg cattctttgg ctgggccttt atcaccagcc
    20581 tacaggctaa acgcggttgg atgcgcgtgg tgcaaataat cctgctggca gcggcattgg
    20641 ttagcgtgcg cccacttcag gattgggcat ttggtgcgac gcataccgcg caaactcaga
    20701 cgcatctcaa ctttacacaa atcaaaacgg tagatgagtt aaatcatgcg ctcgttgaag
    20761 ccaaaggcaa accggtgatg ttagatcttt atgccgactg gtgcgtcgcc tgtaaagagt
    20821 ttgagaaata caccttcagc gacccgcagg tgcaaaaagc gttagcagac acggtcttac
    20881 ttcaggccaa catcacggcc aacgacgcac aagatgtggc gctgttaaag catcttaatg
    20941 tccttggcct accgacaatt ctcttttttg acggacaagg ccaggagcat ccacaagcac
    21001 gcgtcacggg ctttatggat gctgaaacct tcagcgcaca tttgcgcgat cgccaaccgt
    21061 gaacgacact tgcagcggga aagacggagg aaaatagcgt gcaacgtgaa gatgtactgg
    21121 gagaagccct gaaattatta gaattacaag ggattgccaa caccacgctg gagatggttg
    21181 ctgaacgtgt ggattatcca ctggacgagc tacgccgctt ctggccagac aaagaggcaa
    21241 tcctctacga tgcgctgcgt tatcttagcc aacagataga tgtctggcgt cgtcagctga
    21301 tgctggacga aacgcaaacc gccgaacaaa agcagctggc acgttatcag gcgttatcgg
    21361 agtgcgttaa aaacaaccgc tatccgggct gtctatttat cgctgcctgt acgttttatc
    21421 ccgatcctgg ccaccctatt catcaactgg ccgatcagca aaaaagcgcg gcctacgatt
    21481 tcacccacga actgttaacc acactggaag ttgacgatcc ggcgatggta gcaaagcaga
    21541 tggaactggt gctggaaggc tgtttaagcc gaatgctggt gaatcgtagc caggcggatg
    21601 tcgacactgc acatcggctg gcggaagata tcctgcgctt cgcccgctgc cgccagggtg
    21661 gtgcattgac ctgacagaaa cacagaaaag aagcgatttg ccgcaatctt aagcagttga
    21721 atcgctttta ctgaaattag gttgacgaga tgtgcagatt acggtttaat gcgccccgtt
    21781 gcccggatag ctcagtcggt agagcagggg attgaaaatc cccgtgtcct tggttcgatt
    21841 ccgagtccgg gcaccaaatt catatcaacg gacctctacg gaggttcgtt tttcgtttca
    21901 gaacgccacg atttaagcgt tctgcctaca aatcaattct accgaactca accagattcc
    21961 ccccacatca accccattgt gtgggtataa ttgcgggcat accccagttc gacagaattt
    22021 gtaccctctt taacgcattc agaaggctga actatggcac tgactgacgc aaaaatccgg
    22081 gctgcaaagc ccactgacaa ggcttataaa ctcactgacg ggactggcat gttcctgctg
    22141 gtacatccta atggctcccg ttactggcgt ctccgttatc gtattctggg taaggagaag
    22201 actctggcac ttggtgtgta tccagaagtt tctctctccg aagctcgtac aaaacgggat
    22261 gaggcccgaa aactgatttc ggagggggtt gacccttgcg aacagaaaag agctaaaaaa
    22321 gtagtccctg atttacagct ctcttttgaa catattgcgc gacgctggca tgccagtaat
    22381 aaacaatggg cacaatcaca cagcgataaa gtactcaaaa gcctcgagac tcacgttttc
    22441 ccctttatcg gcaaccggga tatcacaaca ctcaataccc cggatctgct tatccctgtt
    22501 cgtgctgcag aagctaaaca aatttatgaa atcgccagtc gtctgcagca aagaatatct
    22561 gccgtaatgc gttatgccgt acagtctggc atcatcagat ataatcctgc tctggatatg
    22621 gctggcgcat tgactacggt aaaacgccag catcgccccg ctcttgatct ttctcgcctg
    22681 cctgaacttt tgtcgcgtat tggcagttac aaagggcaaa ctgtcaccca gcttgccgtt
    22741 atgctgaatt tactggtttt tattcgttcc agtgaactca gatacgcccg ctggtctgaa
    22801 attgatattg acaatgccat gtggactatt ccagccgaac gcgaacccct gccaggcgta
    22861 aaattctcac accggggctc caagatgcga acaccacatc ttgtgccact cagcaaacag
    22921 gctgtagcca tactgacaga actacagaca tgggctggtg aaaatggtct gatctttacg
    22981 ggagcacatg acccgcgtaa accaatcagt gaaaatgtaa tgaacgcatc
//
LOCUS       DAZPEB010000046        22865 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZPEB010000046 DAZPEB010000000
VERSION     DAZPEB010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22865
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.047"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            40..969
                     /gene="aaeR"
                     /locus_tag="ACHURJ_001602"
     CDS             40..969
                     /gene="aaeR"
                     /locus_tag="ACHURJ_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFR1280596.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            complement(1097..2542)
                     /gene="tldD"
                     /locus_tag="ACHURJ_001603"
     CDS             complement(1097..2542)
                     /gene="tldD"
                     /locus_tag="ACHURJ_001603"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFR1280597.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVGHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVRGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(2698..6498)
                     /gene="yhdP"
                     /locus_tag="ACHURJ_001604"
     CDS             complement(2698..6498)
                     /gene="yhdP"
                     /locus_tag="ACHURJ_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFR1280598.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGGNDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFHLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDDVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKLLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPAAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLPVNVKVDGNLSSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDLTISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            complement(6566..8035)
                     /gene="rng"
                     /locus_tag="ACHURJ_001605"
     CDS             complement(6566..8035)
                     /gene="rng"
                     /locus_tag="ACHURJ_001605"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFR1280599.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            complement(8025..8618)
                     /gene="yhdE"
                     /locus_tag="ACHURJ_001606"
     CDS             complement(8025..8618)
                     /gene="yhdE"
                     /locus_tag="ACHURJ_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFR1280600.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAHKKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVVLADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            complement(8627..9115)
                     /gene="mreD"
                     /locus_tag="ACHURJ_001607"
     CDS             complement(8627..9115)
                     /gene="mreD"
                     /locus_tag="ACHURJ_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFR1280601.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            complement(9115..10200)
                     /gene="mreC"
                     /locus_tag="ACHURJ_001608"
     CDS             complement(9115..10200)
                     /gene="mreC"
                     /locus_tag="ACHURJ_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFR1280602.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPTTGIAQPTPQQPTTGNAATA
                     PAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            complement(10266..11309)
                     /gene="mreB"
                     /locus_tag="ACHURJ_001609"
     CDS             complement(10266..11309)
                     /gene="mreB"
                     /locus_tag="ACHURJ_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFR1280603.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNN
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            complement(11614..13554)
                     /gene="csrD"
                     /locus_tag="ACHURJ_001610"
     CDS             complement(11614..13554)
                     /gene="csrD"
                     /locus_tag="ACHURJ_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFR1280604.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKYPGMSLRLVYQDPMGNYFHSLMTTAPLTGAISFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            13706..14680
                     /locus_tag="ACHURJ_001611"
     CDS             13706..14680
                     /locus_tag="ACHURJ_001611"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFR1280605.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVMVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAIIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            15658..16128
                     /gene="accB"
                     /locus_tag="ACHURJ_001612"
     CDS             15658..16128
                     /gene="accB"
                     /locus_tag="ACHURJ_001612"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFR1280606.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            16139..17488
                     /gene="accC"
                     /locus_tag="ACHURJ_001613"
     CDS             16139..17488
                     /gene="accC"
                     /locus_tag="ACHURJ_001613"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFR1280607.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(17580..18627)
                     /locus_tag="ACHURJ_001614"
                     /pseudo
     CDS             complement(17580..18627)
                     /locus_tag="ACHURJ_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787050.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
     gene            complement(18624..19586)
                     /locus_tag="ACHURJ_001615"
     CDS             complement(18624..19586)
                     /locus_tag="ACHURJ_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFR1280608.1"
                     /translation="MSKVFTIGEILVEIMASKIGQPFDQPGIWNGPYPSGAPAIFIDQ
                     VTRLGVPCGIISCVGNDGFGDINIHRLAADGVDIRGISVLPQEATGSAFVTYHNSGDR
                     DFIFNIKNAACGKLSAQHVDENILKDCTHFHIMGSSLFSFHMVDAVKKAVTIVKANGG
                     VISFDPNIRKEMLDIPEMRDALHFVLELTDIYMPSEGEVLLLSPHSTPERAIAGFLEE
                     GVKEVIVKRGNQGASYYSANEQFHVESYPVEEVDPTGAGDCFGGAWIACRQLGFDVHR
                     ALQYANACGALAVTRRGPMEGTSRLAEIEAFIQRHDMSIREAAQ"
     gene            complement(19608..20597)
                     /locus_tag="ACHURJ_001616"
                     /pseudo
     CDS             complement(19608..20597)
                     /locus_tag="ACHURJ_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525780.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(20598..22097)
                     /locus_tag="ACHURJ_001617"
     CDS             complement(20598..22097)
                     /locus_tag="ACHURJ_001617"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFR1280609.1"
                     /translation="MTDVILDVSHIAKTFGHVQALKDITLSLRKGRVHTLLGENGAGK
                     STLMKILAGVYPPTQGTITLRGETITINNPQHSRQLGIAIIFQELSLSNNMTVAENIY
                     ANNEPRRFGIINDKKMLADCQNLLAERGIPLDPLEMVGNMSMAHRQLVEIAKALSYAA
                     DVVIMDEPTSSLSDNEAEILFNIIEKLKQRGCAVIYISHRMDEIMRISDDISVIRDGE
                     YIATHEKKNSDIQHLIAQMVGREMKNIWPARLGEIPDENVPAKLEVKNLSHPSLFKEI
                     SFAVRPGEVLGFFGLVGAGRSDVMKALFGLVSYQGTVLIDGKEVRITNPKQAIDHGIA
                     FVTENRKEEGLVLMHDVNMNTHHVAFQYNASRMGLINHRQEEAKTLQSIARMNTKVSS
                     VHQAVGALSGGNQQKIVLSKWLEKTPRILLLDEPTRGVDVGAKFEIYNVIRQLAAAGT
                     AIILVSSELPEVMALSDRLVVMRNKTIADIYSCENLTQTQVMTAATGVR"
     gene            complement(22158..>22865)
                     /locus_tag="ACHURJ_001618"
     CDS             complement(22158..>22865)
                     /locus_tag="ACHURJ_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFR1280610.1"
                     /translation="LFANQDLNKQLSDVEDFITKKVDAIIISPVDSKGVQAAIIKADK
                     AGIPVITVDVAAEGVPVVSHVATDNYAGGVEAGKLMGKLLNGKGTVAIISYPALQSVV
                     ARVDGFKKGLSDTPDIKIVAEQPSITRAEALTTAQNIMQANANLNGLFGFGDDAALAA
                     VIAAKSAHNDNIKIIGFDGMKEARDAVDSEKTFAAVIRQYPDQMGAKAIDAAVDHLNG
                     KPVEKMIPVQPGVYTGK"
BASE COUNT         5669 a         6193 c         5459 g         5544 t
ORIGIN      
        1 tattatgtga tctaaatcac ttttaagtca gagtgaataa tggaacgact aaaacgcatg
       61 tcggtgtttg ccaaagtagt tgaatttggc tcttttaccg ccgccgccag acagctacag
      121 atgagcgttt cgtccatcag tcagacggtg tcaaaactgg aagatgagtt gcaggtaaag
      181 ctgttaaacc gtagcacacg cagcattggc ctgaccgaag ccggtagaat ttactaccag
      241 ggctgccgtc gtatgcttca tgaagtgcag gatgttcatg agcaactgta tgccttcaat
      301 aacaccccca tcgggacgct acgcattggc tgttcttcaa ctatggcaca aaatgttctc
      361 gccgggctga cagccaaaat gctgaaagaa tacccaggtt tgagcgtcaa tctggttacc
      421 ggaattccag cccccgacct gattgccgac ggtctggatg tggtgatccg cgtcggcgcg
      481 ttgcaggatt ccagcctgtt ttcccgccgt cttggcgcga tgccaatggt ggtgtgcgcc
      541 gcgaaaagct atctcacaca atacggcata ccggaaaaac ccgccgattt aagtagccat
      601 tcatggcttg aatacagcgt gcggcccgac aatgaatttg aactgattgc accggaaggg
      661 atctcgactc gcctgatccc gcaagggaga tttgtgacta atgatccgat gacgctggtg
      721 cgctggctaa cggcgggtgc cgggatcgcc tacgtgccgc tgatgtgggt gatcaacgag
      781 atcaatcgtg gggagctgga gatcctgctg ccgcgttatc agtcagatcc acgcccggtt
      841 tatgcgttat ataccgaaaa agataagctg ccgctgaagg tacaggtcgt gatcaactcg
      901 ctgacggatt attttgttga ggtcggtaaa ttgtttcagg agatgcacgg gcgcgggaaa
      961 gagaagtaat tcatattgta ctgttacgtt ctacaaacct gtgccaacgg gttcccctca
     1021 ccctaacccc agaggagcga ggggaccgtt cgtgcacgta gaaagattaa ttatccttct
     1081 gaaaataatg aaattattac gcagtaccgc caacagtcag gttatcaact ttcagcgttg
     1141 gctggcccac gccaaccggc aaactttgcc cttctttacc gcagacaccc acgccgttat
     1201 ccagtttcag gtcgttgcca accatcgaaa tctgctgcat ggtttcgata ccggaaccaa
     1261 tcaacgttgc gcctctcacc ggcttcgtta ctttaccgtt ttcaatcaga tatgcttctg
     1321 aagtggagaa aacgaatttg ccggaggtga tatccacctg accgccacca aagttcggtg
     1381 catagatacc gtactcaacg gattcaataa tttcctgcgg ggtcgattta cccggcagca
     1441 tataggtgtt ggtcatacgc ggcatgggca gatgggcgta ggattcacgg cgaccgttgc
     1501 cagtcggcgt catccccatc aaacgcgcgt tgagtttatc ctgcatgtag cctttcagaa
     1561 tgccgttctc aatcagcacg ttgtactggc ctggcgtacc ttcgtcatca atcgccaccg
     1621 aaccacggcg atcgaccatc gtgccgtcat caaccacggt acacagttct gaagccacca
     1681 gctccccgac ctgcccacta aatactgaag tgccgcggcg gttgaagtcg ccttccagac
     1741 cgtgacctac cgcttcatgc aacagcacgc ccggccaacc tgcgccaagt actaccggca
     1801 tggtgcccgc tggtgcagca acggcagaaa gattgaccag cgccatacgc actgcttctt
     1861 ttgcccatgc atccgcacgg acttcgccgt caagatcggc aaggaagaac tcataaccaa
     1921 aacgaccgcc accgccactg gcaccgcgtt cgcgtttgcc atcttcttcg accagaacgc
     1981 tcacggaaag acgcaccagc ggacggacat ccgccgctag cgtgccgtcg gtggccgcaa
     2041 ccagaattaa ttcatagaca ccactgaggc tggcagtcac ttcctgtacg cgcttgtccg
     2101 cttcgcgagc aaccttatcg acgcgacgca ggatatccag cttctcttca cggctcatgc
     2161 tttgcagcgg atctaccgag gtatacaacg ggctatgccc taccgcgccc agcgtctgta
     2221 ctttgccatc accactatca cggacgatgg tgcgcgccgc ttgcgcactc tgttccagcg
     2281 ccagcaggct gatttggtca gcgtaagcaa atccggtttt ttcaccgctg attgcacgca
     2341 cgccaacgcc ctgatcgatg ttgtaagaac catctttaat aatgcggtct tctaaaaccc
     2401 aggattcgtg atagctcgac tgaaaataga gatcgccata atcaaggcga cgttcggcca
     2461 gttgaccgag gatcgcgaac aagtcctgat gtttcaggcc gttcgccgct agcaattgtt
     2521 cacttactag gttaagactc atcgtttttg ctactcgtta gttactgcag tagaggattt
     2581 tttacggctg ccggatgcgg cgtgaacgcc ctatccgacc tacggttatg ttctggctcg
     2641 ttggcctttg gcaacgatta tcatatgaga ttggggcaat tacgcgccct cgtcaaatca
     2701 ttgcgctttt tctttacgcg gttggcgcaa cacttcgttg atttgcggat cgtccagcgg
     2761 acccgaaatg tgatagcgca aaatggagac tttgctccac agcggcccca gcactttact
     2821 ggctgcaaat actgccgcgc caacaatggg gttaaccgca aaagccgcag ccacgcccac
     2881 cgtcgcagaa atctctggtg cgacaaccgc ttccatattc aggtcgcgac gtaccagatt
     2941 taccgaccct ttcatggcga tatccgcctc caggccatcc accagcgtgt cgtcggtgtg
     3001 cataacgccg tctttaatcc acgcggtgct gcgaatggag tcaaaataga acccttcgcc
     3061 aaaagtgtct ctgaaatcaa aacgcagctt acgcatcagg gcatcaacgc tcaataagcg
     3121 cagcaattgc ccggcatgtc cggtattgat ttcggtaatt tcgcctttac ccagttgagt
     3181 atgaatgatg ccattcaacg tcgcctcatc tggttgccag ggtgctttgc gccagtgtaa
     3241 atcgtaatcc acattaaatg acgactggcg tatgggcgtc gtgacaccaa aaaattctgc
     3301 ggcggcatca attttctgcc cgcgcagttt tcctttcagc gaggtacgtt cattccccgg
     3361 attattaacc cattcaccat cggcagtaag ccgcgagaaa ccagtatcaa tcagtccatt
     3421 ggtcagcgtt aacgtatcgc cagaaatggt gagatcactg tcaatacgac cgaatttttg
     3481 cccccagaac cagcactctg tgcatcgtat ttgtgcgtcc ggccagccac ggaagttaat
     3541 gcgctccgtt gtcgggaaag gtgatgacgg tgttgaatca ccacgagttt tcgccacgct
     3601 cgggttgtaa taaagatatt tgatattcgc cagccacggc gcgttattac gcatcgccag
     3661 cgtggcattg atttcacgcc cctgtgcctc aaccagggtg ccatttgccg tcggttgcga
     3721 aacaatactc aggttattcc attgctgatt tcccagtgac aacataggcg tacgtaacgt
     3781 tatgtgttgt gggaaactcg ctgcaccacc gacactctcc gcagcgcctt tctggaacag
     3841 ggccagccac tcggcaccat tcatcggcgg catattgagt tcaacgccac tttgttccgg
     3901 caacggcggg agcgttttac tgtctgccgc ccaaatagca cgatcgagcg tcagcttttg
     3961 accaagcaac cagcggctat tgaaatggtt atccgcacca gcctgtccgg ttaattcaaa
     4021 gctgctgaga ttgccatcaa ccttaacgtt taccggcagt ggttcacccg caggtttggc
     4081 taacggtgaa ggtaagtgac tgctcacatt attcagatcg ccgttcagct ctacgttata
     4141 ggtcgcgcca gcatgataag gcagatcaat gcccacttta ccatcccacg ccacgctgcc
     4201 actcaatgct tcgttcaccg ctgcaggcag aacgccggtt ttcgccggtt gccagttacc
     4261 gttgaggttt accgcaacct ggtaggcttt tgccccttct ttggtggaaa aatccacgtt
     4321 caacggctga ttaaaccagc ttgctgtcag tggttcactt tgcagatcgc cattgataaa
     4381 gctgaattta ccgctcaaat ttttcagggt gctgtcgagt agtttgataa acagactgtt
     4441 attacgcagc gtcacttcac ctttcgcggt taccagttcg ccgttcagcg ggatatcaag
     4501 atgtaagcga gcattcacat cgccgtcgag ctggagttct tgcagggtcg cacccagaga
     4561 atctttcagc ggtgtctcat caaagtaagg gccaacggct ttacccggac ctttaatgtc
     4621 agcgtcaatc agcagttttt ctttcgagta gtcagggatc actgcggtga gattactcgc
     4681 gcgcacgccg cccagattaa cgtcatcggt tttcatccat aaaccgtcgt taataaagtc
     4741 cagttcaata tcaaggttag ttaatgcagg ccagtccggc tggaaggcaa acttcgcgtt
     4801 acgcagcggc accagcactt caaactgacc ttcgttgtgt ttatagggga agagttgcgg
     4861 attgccacca taaaccagcg tcgcgttatc cgcttcaccg ccctgaatcg cgccacttaa
     4921 gtaatcaact aggtctttac ccatcaagtt ttccgggaaa tagcgccagg cttgtgaacc
     4981 atcatcggta ctgatgccag ccagaatacc cagccagggt tcatcgttag caggttgcag
     5041 gtaacgaaaa ccgccgcgcg catggacagc tttggcttta acgtcaatat tacgcccatc
     5101 caggtggaaa cctttgtcat tgttcagcca gcttatagtt gcctggccgt cggcgatttc
     5161 cagtggcgca cggaataccg tttcgtaagg catctttgcc tgcttcatcg acgcggtaag
     5221 caaaccattt tcaacgctgc cggaaagcgt cccggagaag tgttccgcac ccggtaataa
     5281 tttccattgc ttccaggcca gatcgctcca cgatgcctga aaacgggtct tgtctgccgc
     5341 ctgaagcggg atatccagcg ccagagtgtt aatcttgccg ctcggttgtg tggagcgcca
     5401 aacatcaccc agtgcaggtg aaagtttcgc ggccagcggg cgtatgccct ccaggcctgc
     5461 cagctccaga ttactggcgc gaatccggag ttcgtcactg cgtttattgt ctttgccgcc
     5521 aacgtcctgt tccggtatcc aggccagcgt caatgctccg ctcggccagg gtttgccgtc
     5581 catcgtgatc cgtgtatcgg gaatagagaa ctgccagccc ggattttcac gcgtaatatg
     5641 cgcggtcaga ttatccaccg acagcgtatg cgtttgcttc tcgcccaacc agctggcacc
     5701 gccctgtttc agccagacgt caccgccggt tacatcgcct ttgtcgatcg tcatccagcc
     5761 ttcaagggag aactgtgccg tttccagagc aatattgtcc tgcatccatt taccgagcca
     5821 cggcttcagg tcgatgtcat ccgcctgtag ccagacgcga ccattgctta acaacccctc
     5881 atcatcgcgc aaatccatgc gcacctgcat cacgccgtgc tgtccggtaa ggctggagag
     5941 gcttaccagg ccttccgcac ggtgtcgacg tggatcgttc agccaggtga gttgtgggat
     6001 cgccagctcg gcgcgctgac cggatggcgt cagaaaactg acttcactgt cgcggagatc
     6061 gaaatggtca aattgacgaa gaaacagatc gctgatgtga ctggcttcca ggctatcatt
     6121 accaccaccg ctggtgatag gagtgttggt gcgaaagcgc agctgccaga aagtgaggtc
     6181 gcgaaactgc cagcgcatat gtaacaagct ctgccagaca tccagcgcca gagtaacgcg
     6241 tttaaccgaa aattcgccgc catcttttag ttctgcacgg atgtcgtgtg cttcaagcgt
     6301 cgggccaaaa ttctgccagc tggctgagag ctgactggct tctaccggca tgccagtcgc
     6361 ggattctatt ttgttgagga tttccggacg ccaggcgtca agatgcggta aagcaatacg
     6421 caggccgcta accagcaggg ctgcgatcac aacgagcgcg gctccagtaa gcagtaaaat
     6481 ccccggcaat cgcctcaccc gtcactcctt gtctgctaaa aatgtgactc aaaaaccctt
     6541 tgccggatgg cggcccagca tctgtttaca tcattacgac gtcaaactgc tcctggttat
     6601 agagcggttc aatttgtact ttaacctgtt tgccaacgaa aatttccact tccgccagcg
     6661 agtgtgactc ttcgcctttc aaggcttcag ctactgccgg agaagcatag accaggaaac
     6721 ggtcggagtc gtaagcatgg tggacacgaa caatctcgcg catgatttca tagcataccg
     6781 tttccacggt tttcaccgtt ccgcgaccgt ggcaggttgg gcattcgtta cacagtacgt
     6841 gctcaatgct ttcgcgggtg cgtttacgcg tcatctccac cagccccagc gccgaaaaac
     6901 cattaacgct ggttttcacc cggtctttgc tcaacgcctg ctccagcgag tgcagcacgc
     6961 ggcggcggtg atcttcatta ttcatatcga tgaaatcaat aatgataatc ccacccagat
     7021 tacgcaaccg taactggcga gcgatagcct gcgtcgcttc aatattggta ttgaaaatgg
     7081 tgtcgtccag attgcgatga ccgacaaacg ctccggtatt gatgtccacg gtggtcatcg
     7141 cttcggtctg gtcgataatc agataaccac cggatttcag ttctactttg cgttccagcg
     7201 ctcgctggat ttcgttttcg acatcaaaga gatcgaaaat cggctggcgt ccggtgtaat
     7261 gctccagctt gctggtcatc tcgggaatgt actccgaggt aaactccagc aatgcttcgt
     7321 aagtcaggcg tgagtcaacg cgaatgcggt ccagttcggc gtcggcgaaa tcacgcagaa
     7381 cacgctgcgc cagcgccagt tcgccgtaca gctgataacg ggtctgcggg cgttttttac
     7441 gctccataac tttggtccag acgcgtttca gataagcggc atcggaggcc agttcagcct
     7501 cgccaacccc ttccgctgcg gtacggatga taaacccgcc ctgctcgtcg caatactctg
     7561 cgaccacttt tttcaggcgt tcacgttctg attcgctttc aatacgttgg gaaaccccaa
     7621 cgtgagaagc ccctggcata aacaccagat agcgagaagg cagcgtgata tcggtggtca
     7681 ggcgcgcacc tttagtgcca agcggatctt tcaccacctg caccatcaga tcttgcccct
     7741 gacgaaccag ttccgagatg tcgcgcaccg tgaattgctt ttgttcttca cccgccacac
     7801 attcggtgtg cggcatgata tcggatgcat gaagaaacgc ggctttatcc agcccaatat
     7861 ctacaaaagc cgcctgcata cccggaagta cacgacttac acgacccttg tagatattgc
     7921 ctactattcc gcgtcgcgcc tcacgttcaa tatgaatttc ctgcagaata ccgccatcaa
     7981 tatacgccac tcgcgtttcc gaaggcgtta cgtttactaa caattcagcc gtcatgttta
     8041 tcccttttct cacgcagtgc gttaaaatta cttaataatt catacgtttc aaccagcggt
     8101 aagccgacta cggcgtgata gctgccattt atcttcctga caaaacagcc acccagcccc
     8161 tgaataccgt atgcacctgc tttatctaac ggttcaccgc tggcgacata gcccgcgatg
     8221 tcttcgtctg ttaacgttct gaaagtcaca tcggtgacca ccaggcaatc gagaatgtgc
     8281 tggctgtcgg ccaacaccac tgccgtcatc acctgatggg tctgacccga taatttgcgc
     8341 aacatctgcg ccgcatgctc tgcgtcgcgc ggtttctcca gcacttctcc attcaggata
     8401 acgatagtat ccgcacccag caccgggaga tcctgcgccg tttgcgcgac acccgcctgt
     8461 gctttcttgt gtgccagacg cacaacatac tgctgcgcgc tctcctgcgg ctgacgctgc
     8521 tcctcaatgc ccgtaacaat acgttcaaag gtcacgccaa gttgcgcaag taactcctga
     8581 cgacgcggag aaccggaagc taaatacaga gaagtcatag aaacctttat tgcactgcaa
     8641 actgctgacg gactttacgc atcagcaaga aaatccacgg ccagagcacc ccattgacta
     8701 cactactcca gaacacttcc ggtctgaaag agacgttaat cactaaaaac tctgcccaga
     8761 aaacaataat atccaccacc agcgaaagca acatgacgac tagcgcctgc tgccataatg
     8821 cgaggttgcg gaaaagctgg tatttcagcg ccaccaggta agcaatgatg ctcatcgcca
     8881 atacgcgtac gccaagcgtc gagccgctga tcagatccag tatggcaccc atcacaaaac
     8941 ctgtgcccac atttacgcga tggggcaagg ccaggatcca atacaacaag ataagtaaca
     9001 cccagtttgg ccggaaaaca atcaggttat ccggccaggg catgatttgc agcaacagcg
     9061 caatgaggaa agagagccag attacccaac gtccctggct acgatagctc gccactattg
     9121 ccctcccggc gcacgcgcag gcggttgagc accactttgc ggcggcgtag ccctttgtgg
     9181 agagcgatta gcagcaggcg cagtagctgc atttcctgtc gtcggttgct gcggagtcgg
     9241 ctgagcgatc cccgttgtcg gttcaggtaa ctttggcccc atcgcgtctg gcgaaggcaa
     9301 tacctgcggc atcatctgca tcagacgttc attagcaaca cgatgcacct cttccggcgt
     9361 catcgggtta gcgccgttac gatccgcgcc ccacagcagc agcagataac gcaaacgttg
     9421 cagtcctgca gtcggacgcg cctgaatcac agtataagcg cgctgggtat cgagttttac
     9481 ggaagagaca accgcgaccg gatagccttc cgggaaacga ccgcccagac cggaggtcac
     9541 cagcacatca ccaacacgaa tatccgtatt cgctggcaga tgctcaagct gcaaatcatc
     9601 tgtacaaccg ttaccggctg caattacgcg gatatcgttg cgcagcacct ggattggcag
     9661 cgcgtgggtc gcatcacaaa tcagcagcac gcgactggtc agtttagcga cggccaccac
     9721 ctgaccaaca acacctttgt cgctgatgac cggctggcct tcataaacgc cattaacgct
     9781 acctttatcg ataacaactt gatcgctata aggatcgtta accgtggaga taacctgagt
     9841 caccattttc tgctcatcct gacgcagcgg ggaacccagc agctcgcgca gacgcgcgtt
     9901 ctcctgtttg tattgtccaa gcatcagcag ttcactgttt ttcagcaaca gttcctgacg
     9961 taacgcccgg ttttcaagtt ctagttggtc acgcgaggcc agcgtctgcg atacgccatc
    10021 cagcaattca cgaggagcat tggaaacaaa gtagaaagga ctgacggcgg tatccatata
    10081 agtacggatt tgactgaacg tccccaggcg gctgtcggca ataataatgc cgagcgccac
    10141 cagcaccgcc agaataaggc gaatctgtag cgacgggcca cggctaaaaa ttggcttcat
    10201 aagttatgcg tattctcgta tcagaccagg cagggtaaac agacatttcc cctgcctgca
    10261 tccgattact cttcgctgaa caggtcgccg ccgtgcatgt cgatcatttc cagcgctttg
    10321 ccgccaccgc gcgccacaca ggtcagcggg tcttcagcaa caacgactgg aatgccggtt
    10381 tcttccatta acaaacggtc aaggttacgc agcagtgcgc caccaccggt gagcaccatg
    10441 ccgcgctcgg agatgtcgga agccagttcc ggcgggcact gttccagtgc aaccattacc
    10501 gcgctcacaa taccggtcag cggttcctgc agtgcttcga ggatttcgtt ggagttcagg
    10561 gtaaaaccgc gtggaacacc ttctgccagg ttacggccac gaacttcgat ttcacggact
    10621 tcatcgcccg ggtaagccga accgatttcg tgcttgatac gttctgcggt ggcttcaccg
    10681 atcagagaac cgtaattacg acgcacatag ttgatgatag cttcgtcgaa acgatcacca
    10741 ccaatgcgca cggaagagga gtaaaccacg ccgttcaagg agataacagc aacttcagtg
    10801 gtaccaccac cgatatcaac caccatagaa ccggtcgctt cagaaaccgg caggccagca
    10861 ccaattgcgg cagccatcgg ttcttcaatc aggaagactt cacgggcacc agcgccctgc
    10921 gcggattcac gaattgcgcg gcgttcaacc tgggtcgcgc caaccggtac acaaaccaga
    10981 acgcgcgggc ttggacgcat aaagttgttg ctgtgcactt gtttgatgaa gtgctggagc
    11041 attttttcag tcacgaagaa gtcggcgata acgccgtctt tcattgggcg aatggcagca
    11101 atattgcccg gcgtacggcc cagcatctgc ttcgcgtcat gacctactgc agctacgctt
    11161 ttcggtgaac cggcacgatc ctgacgaatg gccaccacgg aaggctcatt caatacgatg
    11221 ccttgtcctt ttacataaat gagggtattc gcagtaccca ggtcaatgga caagtcattg
    11281 gaaaacatgc cacgaaattt tttcaacata ctaagggata atcctgaaag ctggggcgga
    11341 aaagaaaatc cgcttacttt accaaccaca cgcagcagcg acaaggcgca aaaatcatct
    11401 gctacggtga aaattagtgc agttcgtttc ctttgttaca aatctctgcc tgagtccaga
    11461 aaggcttaat gcatcagcag cattcctcgc ctgtttgcaa ccgcgtaagg tcattcatct
    11521 gcatatgtgc tgcaacaatc tggcgagcag acaagcacac tcccatgaga cgcagcgcgc
    11581 gttattctac gtgaaaacgg attaaacggc aggttaaacc gagtatcttt gtgaatattt
    11641 tttcacgtta gtatcaagtg gctgtgagga cgcgaaaaaa tccccttgcc cgcctgtaac
    11701 accgcgctga atcagggtct gccactcgct tcgcgaacgc acaccggtgg cgtaaacctg
    11761 ggtgctggtc ccggagcagg cttccaccag gctttgaacc agcagctggt tctccgttcg
    11821 cttctcaatg tttctgacca gccccggatg gagcttgagt aactcaacat taagttcttt
    11881 gatccaactg gtgcttacca gcgtcaaacc agcctggttg acggctaccc gtacccctaa
    11941 agcattcact aaacgaataa caggttgtaa acgactgata tgttgaccta catcggcctc
    12001 tgcaagttca ataattatgc gttttcgttg tgatttttca cattgcatta acgtatcgcg
    12061 cagccaacgc tgaaaacgcg ggcgaatcag cgactcaacg gtaacctgaa tcgccagatt
    12121 ttcctctggc cagtaacgca atagtggaat aagacggctg atttgcagac ggtcatactc
    12181 ttccgataag ccaaactgca agaccatcgg catatactcc gccgagctaa cctcttcatt
    12241 accatcgaag atgcggcaca tgagttcgcg atgatgaacc tgaccttcgc gagtaaccgc
    12301 cggtttttga taaaggcgcg ggccgccgcg actgagcatt tgctcgataa gcgtacgcca
    12361 gcgaacatta ccgcgtcctt tttcaggcaa cgagtcatcg taaatagccc agctattgcc
    12421 gccctgcaat cccgcattac gcgtggcaga ctctgcatgt tccattacct gctcggtatc
    12481 ctgaccacta cgccaggcgc agataccaat gtggatcata tcgtcgcgat cgagcatttt
    12541 attgttcggc aaggtatcga cggctttgat taactgaccg gcgatgctct ctgcctcttt
    12601 taacgtccgg tgcggtaaca gcgcagcaaa atcactgcgg tggtaacgcg ccagcagtgc
    12661 gccagggtag cgcatcataa atgtcgacag cagattcgtc agagtgaaga actgttcttc
    12721 aacctggctg tgcccccaag tatcgctcaa catattgaaa tccggcagac gaatcatcat
    12781 cacgatcccg tgggtaccta ctttctcctg atcttccagt aacgttgcta actgattatc
    12841 gaaaaagagt cggttattga ggccggtttt cacgtcctgg gcggcataag agcggatcag
    12901 cgtatcaaga cggctgtgtt gttcgcgtgc gttctgaatt tcacgaagca gcgtatccag
    12961 cgcactgctg gttctgggcg gccattcata gatggttccc aacacattag agccacgctc
    13021 accgtttaag atacgagtag cccgggtttc cagcaattct tgcccggcaa gttgccgttg
    13081 taaccagcgt accgccaaga agagcataac aatgataaag ctaatcgccc ccgtgagcgg
    13141 cgcggtggtc atcaacgaat ggaaatagtt gcccatcgga tcctgataaa ccagacgcag
    13201 cgacatcccc ggatacttta tcaacggaac gctcagttca cgaaacagat cgctggagcc
    13261 aaccggacga taactaccat ttctggccag ggtataaacc tgtttatcac catggagcag
    13321 gtctacacga acgatatctg ccgacatcat taattcggta atttgtggcc ttaatgtgct
    13381 gaagtcattc gacacaaggt gggtatcaat cgccgtcgcc accgcctgaa cgcgatgact
    13441 aaacttatac tgaattgcgt tgtagaaact tagcgaacag cccagcaaag tcacaaaaat
    13501 tgttaacccg gtgagcagcg taacaaaggc cgaaaatttc gtcgttaatc tcatccttgt
    13561 gttaactccg atagtgagga agcgggcata ctagcaaatc agatttatct cgcaatttat
    13621 tgcgcttcat cggctttgct tttccattag cgagtataat cttcagaaat tattttccaa
    13681 tccatcatgc acatgaggac cacttatgca ggcgttactt ttagaacagc aggacggcaa
    13741 aactctcgca tcagtacaga ctctggacga aagtcgcctg ccggagggcg atgtcatggt
    13801 cgatgttcac tggtcgagcc tgaactataa agatgcgctg gcgattaccg gtaagggaaa
    13861 aatcatccgt aattttccga tgattcctgg gatcgatttt gccggaactg tacgcaccag
    13921 cgaagatccg cgttttcatg ccggtcagga ggtgttactc actggctggg gcgttggtga
    13981 aaaccactgg ggtgggctgg cggagcaggc gcgagtgaaa ggtgactggc tggttgccat
    14041 gccgcaaggg ctggacgcgc gtaaagcaat aattatcggt actgccggtt ttaccgccat
    14101 gctgtgtgtg atggcgctgg aagatgccgg tgttcgcccg caggacgggg agattgtcgt
    14161 gacgggtgcc agtggtggcg tcggcagtac cgccgtggcg ctgctgcata agttgggtta
    14221 tcaggtcgtt gccgtttccg gtcgcgaaag tacccatgaa tatctgaaaa gtttaggtgc
    14281 cagccgtatt ctccctcgtg atgagtttgc cgaatcccgt cctctggaaa aacaagtctg
    14341 ggctggggca attgacactg ttggcgacaa agtgctggca aaagtgctgg cgcaaatgaa
    14401 ttacggcggc tgcgtggcgg cctgtggtct ggcgggtggt tttactctgc caaccacggt
    14461 catgccattt attctgcgta atgtccgttt gcaaggggtg gattcagtaa tgacgccacc
    14521 agaacgccgc gcacaagcct ggcagcgact ggtcgccgat ttaccggaat cattctatac
    14581 ccaggcggca aaagagatat ctctgtcaga ggcaccgaac tttgccgagg ccatcattaa
    14641 taaccagatc cagggtcgca cgctggtgaa ggttaactaa ccatttagca aggaataata
    14701 agagagggaa ctcattttga catcattatt caatcggtta acgggaaaag cggttagccg
    14761 gacagctttc gtcgaacacc ttggtcagga agttgtacaa catcatccaa actggaaagt
    14821 catgatttcg actgaccaca aattgatgcg cattgatact cctctaaaca gccattattg
    14881 atacgcctcc gtcgcctgtt aggtttatgg tgctttgcct gggcgacact acgcttaacc
    14941 tgttactgat ttctggtacc atggggtgaa taatctgatt ttatttgact acaaattaac
    15001 cactcgaacc tatttaatgc tgagaattgt cagtcggttg attttgcgtg ctttagcatt
    15061 cacatctatc cagacgatgc agtgaaaatt gggtaatccc cagcaaccgc tgcgtaatgt
    15121 cgtctatctt gtcgcgatcc tggcatccct acattatttg tggtctgtga agattatctc
    15181 attgcagccc ctcatcttcg cagggctggc tttacagctt ttcaccttac gttataagaa
    15241 gttccgtcga tgatggcgct aatttcgtga attgtgcggc ttgttgcaaa ttacacggtg
    15301 ttgaaggtta tttacatgtt agctgttgat tatcttccct gataagacca gtatttagct
    15361 gccaattgct acgaaatcgt tataatgtgc gacctcgtcc tccctgacgc agtttttgcg
    15421 ctgcggaaaa ggtgacattg gcgcaacgaa ggtatatttt gttttttgcc ggaggatagc
    15481 agcagatcgc tgcacaatgt ccgtcaagtc taacattgat actctggggc aaaatagacc
    15541 ggcgtcccgg cctgctggaa tttatcgcta tgcatacagc tgtcggggca tacgctttac
    15601 agacggcggt gaaacgcctg tcacaatcac actaaacaaa gagtacggaa cccactcatg
    15661 gatattcgta agattaaaaa actgatcgag ctggttgaag aatcaggcat ctccgaactg
    15721 gaaatttctg aaggcgaaga gtcagtacgc attagccgtg cagctcctgc cgcaagtttc
    15781 cctgtgatgc aacaagctta cgctgcacca atgatgcagc agccagctca atctaacgca
    15841 gccgctccgg cgaccgttcc ttccatggaa gcgccagcag cagcggaaat cagtggtcac
    15901 atcgtacgct ccccgatggt tggtactttc taccgcaccc caagcccgga cgcaaaagcg
    15961 ttcatcgaag tgggtcagaa agtcaacgtg ggcgataccc tgtgcatcgt tgaagccatg
    16021 aaaatgatga accagatcga agcggacaaa tccggtaccg tgaaagcaat tctggtcgaa
    16081 agtggacaac cggtagaatt tgacgagccg ctggtcgtca tcgagtaacg aggcgaacat
    16141 gctggataaa attgttattg ccaaccgcgg cgagattgca ttgcgtattc ttcgtgcctg
    16201 taaagaactg ggcatcaaga ctgtcgctgt gcactccagc gcggatcgcg atctaaaaca
    16261 cgtattactg gcagatgaaa cggtctgtat tggccctgct ccgtcagtaa aaagttatct
    16321 gaacatcccg gcaatcatca gcgccgctga aatcaccggc gcagtagcaa tccatccggg
    16381 ttacggcttc ctctccgaga acgccaactt tgccgagcag gttgaacgct ccggctttat
    16441 cttcattggc ccgaaagcag aaaccattcg cctgatgggc gacaaagtat ccgcaatcgc
    16501 ggcgatgaaa aaagcgggcg tcccttgcgt accgggttct gacggcccgc tgggcgacga
    16561 tatggataaa aaccgtgcca ttgctaaacg cattggttat ccggtgatta tcaaagcctc
    16621 cggcggcggt ggtggtcgcg gtatgcgcgt agtgcgcggc gacgctgaac tggcacaatc
    16681 catctccatg acccgtgcgg aagcgaaagc tgctttcagc aacgatatgg tttacatgga
    16741 gaaatacctg gaaaatcctc gccacgtcga gattcaggta ctggctgacg gtcagggcaa
    16801 cgctatctat ctggcggaac gtgactgctc catgcagcgc cgccaccaga aagtggtcga
    16861 agaagcacca gcaccgggca ttaccccgga actacgtcgc tacatcggcg aacgttgcgc
    16921 taaagcgtgt gttgatatcg gctatcgcgg tgcaggtact ttcgagttcc tgttcgaaaa
    16981 cggcgagttc tatttcatcg aaatgaacac ccgtattcag gtagaacacc cggttacaga
    17041 aatgatcacc ggcgttgacc tgatcaaaga acagctgcgt atcgctgccg gtcaaccgct
    17101 gtcgatcaag caagaagaag ttcacgttcg cggccatgcg gtggaatgtc gtatcaacgc
    17161 cgaagatccg aacaccttcc tgccaagccc aggcaaaatc acccgtttcc acgcacctgg
    17221 cggttttgga gtgcgttggg agtctcatat ctacgcgggc tacaccgtac cgccgtacta
    17281 tgactcaatg atcggtaagc tgatttgcta cggtgaaaac cgtgacgtgg cgattgctcg
    17341 catgaagaat gcgctgcagg agctgatcat cgacggtatc aaaaccaacg ttgatctgca
    17401 gatccgcatc atgaatgacg agaacttcca gcatggtggc actaacatcc actatctgga
    17461 gaaaaaactc ggtcttcagg aaaaataatt tgtgacgcgc ggtgttttca tcgcgcttag
    17521 caaagaacgt aatcaacgcc gggcctgtta acgctgcccg gcgtttttat tgccgttagc
    17581 taacggtagc ctgcaatttc cgctttcaac gcgctcattt cagcatcgcc atcaacaatt
    17641 cccagctcaa agcagtattt ctttgtttcc cctggctcaa taaagggcag acgcccctgc
    17701 gctcgttctt tatcacgacc gtcaggaaaa ctattactga cctccagtcc cataacatac
    17761 tcacccgcgc ctaaattttt ccactggata aagttgggta gctgcatggt gtcgaatttc
    17821 tcgtaaattc cccaccccag ttcggcgttc accagagcaa ctttactcat cccctgactg
    17881 tcgctattca acgtgagata gtaaacctgc tcgtcaaaac cgggctgtgg ggtacaaatc
    17941 tccagatgac tggttaaccc ttccgccgca tgcggagttc gtggcgttac gcttttagcc
    18001 gacagcaaga tttctggcct gtggcgataa taaaggccag ccgtaattaa agtgataaag
    18061 aatttgcagc ggctggcggg tgaaaccatg attagtcacc ttgtcttcaa tgcgcagcac
    18121 attttcgcca taacggcagc gaatggtcct ttccagcgtc agattttcgc cgaacagtcg
    18181 cgtttcccgt actttgccat tgatgacaaa ttcaatgccg tcgtcggtcc gttctgtgcg
    18241 ataccccacc tcttctgctg gagtcgcagc caaacgcccg tgtaacccca gcgtttctcc
    18301 ttcatcctca caaggcgtgc caatgtagct aagtccgcag gtagtcagta accccgcgta
    18361 aaagctacgt aaaaatccgt tgccctgaga ctcaaaaaaa gcgggggcga caatgccgtt
    18421 gggggtgata aaagaaagcg ccgtgtcctt gtagctggcc catgcaatat ccagcgcgcg
    18481 atcttttacc accgtaaatg ccagtcccgc accagtacgg acatcaaacg cttctgtacc
    18541 ctttgctctg ccactacgta actcatagtg tttaattccg gcaacctgcg agagatcgcc
    18601 aatccacgac caaatttcgt ttttcattgt gctgcttccc gaatggacat gtcatggcgc
    18661 tggatgaacg cttcgatttc cgcaaggcga gacgttccct ccattgggcc acgtctggtt
    18721 accgccagcg caccgcaggc attggcatat tgcagcgctc gatggacgtc aaaccctagc
    18781 tggcggcagg caatccacgc gccaccaaag cagtctcccg caccggtcgg gtcaacttct
    18841 tccaccggat agctctcaac atgaaactgc tcattcgcgg agtagtaact ggcaccttga
    18901 ttgccgcgct tcacaatcac ctctttaaca ccttcttcaa gaaaacctgc gattgcacgc
    18961 tccggcgtcg aatgtggcga caagagcaac acttcgccct cgctgggcat atagatatcc
    19021 gttaactcaa gaacaaagtg gagggcgtca cgcatttctg gaatatcgag catctcttta
    19081 cgaatattag ggtcgaaaga aataacacca ccatttgctt taacgatagt taccgctttt
    19141 tttactgcgt ctaccatatg aaacgaaaac agggaagagc ccataatatg aaaatgggta
    19201 cagtccttca gaatattttc atcaacatgc tgcgccgata atttaccgca agcggcattc
    19261 ttaatattaa aaataaaatc gcgatcgcca gagttatgat aagtaacaaa ggcactcccg
    19321 gtagcttcct gaggtaagac tgaaatcccc ctgatatcca caccgtcagc agccagacga
    19381 tgtatattaa tatcaccaaa gccatcattc cccacacagc taatgatgcc gcacggaacg
    19441 ccaagacgtg tcacctggtc gataaaaata gcgggcgcac cactgggata agggccgttc
    19501 cagatacctg gttgatcaaa tggctggccg attttactcg ccataatttc aaccagaatt
    19561 tctccaattg tgaagacctt actcatatga ttaattcctt gaaatgatta gctgcgtttt
    19621 cttttatttc ttacgtcaaa ataaacggct acaagaatga ctaatccttt aaccaccatc
    19681 tggtaaaaat agggaacatt cattaagttc atgccattat taataacgcc gatagttaat
    19741 gcgccgatca ccgtccccac cacggttcca tagccgccgt tcaggctggt gcctcccagt
    19801 acagccgcag caatagcatc cagttcgtag ctcaacgcgg aattcggtta cccggaatag
    19861 aggcgtgagg cgaggatcat cccgctgata cctgccagca cggctgtaat cataaagact
    19921 ttaattttca agcgggtgac atttatccct gaataaattg ccgcttcttt attaccacca
    19981 gtgatataaa tttttcgtcc aaaggttgtt ttagtaagaa tgaagtggtt aatcagcaat
    20041 aagatgatta atatccagat tggaataggt atatagagaa attctccatt ccccagtgcc
    20101 agaaaggcat catctttaat catgatgggc ataccatcgg tcactatata agcgatacca
    20161 cggaagatcc ccatagtagc aacggtgaca ataaacgacg ggatccccgc tatagccgtc
    20221 aagccaccat ttatcatccc cagagccagt gctgcaataa tggataatgg gaccaataaa
    20281 ataaatggaa ttccccatga agccccaagc gcagcaaatg tgccaatcaa tgcaatggtt
    20341 gaaccgaccg ataaatcaat atcgccaagc aaaagtacat aagtcattcc ataggcggta
    20401 attgcaatta tcgaaacctg aagaataata ttagtaatgt tgttggcggt aaaaaaactg
    20461 tcattaataa aactgaacag aatgattaac accaccattc cactgaggat accgccatat
    20521 tgcctgaaga cttttttaaa attatttgca atgggaattt ccgaaggttt cggactggca
    20581 agagttttct ctgtcattta acgtactcct gttgcagcgg tcattacctg tgtctgcgtt
    20641 aaattttcac atgaataaat atcggcaatc gttttattac gcattaccac cagacgatcg
    20701 ctaagtgcca tgacttctgg cagttcagag gacaccagaa taattgctgt ccctgccgca
    20761 gccagttgac gtatcacgtt atagatttca aattttgcgc cgacatccac tccgcgcgtt
    20821 ggctcatcga gcaataaaat acgcggagtt ttctccagcc acttcgataa cacgattttc
    20881 tgttgattac cgccgctcag cgctcccact gcctgatgta cagaactcac tttagtgttc
    20941 attctggcaa ttgattgcag cgtcttcgcc tcttcttgtc ggtgattgat gagtcccatc
    21001 cgcgaagcgt tatattgaaa cgccacatgg tgcgtgttca tattgacgtc gtgcatcagc
    21061 actaaccctt cttccttgcg gttttcggtc acaaaagcaa tgccatgatc aatagcctgt
    21121 ttaggattag tgatcctgac ttccttacca tcaatcaaca cagtcccctg atacgaaacg
    21181 aggccaaaga gcgccttcat cacatcggaa cgcccggccc ccaccaggcc aaaaaatccg
    21241 aggacctccc ccggacgcac agcgaaactg atctctttaa aaagcgaagg atgggaaaga
    21301 ttttttactt ccagttttgc aggaacattt tcatccggta tttccccaag acgtgctggc
    21361 caaatatttt tcatttcccg accaaccatt tgggcaatca aatgctggat atcactgttt
    21421 ttcttttcat gcgtggcgat atactcaccg tcgcggatca ccgaaatatc atctgaaatc
    21481 cgcataattt catccatgcg atgagagata taaataaccg cacagcctcg ctgctttaat
    21541 ttttcaataa tgttaaacag aatctccgct tcattatcac ttagcgaaga tgtcggttca
    21601 tccataatca cgacatccgc tgcatagctt aaggctttag caatttctac cagttgccta
    21661 tgtgccatcg acatattgcc gaccatttct agtgggtcta gtggtatgcc ccgttccgcg
    21721 aggagattct ggcaatctgc gagcattttt ttatcgttaa taatgccaaa acgtcgcggt
    21781 tcattattgg cataaatatt ttccgccacg gtcatattat tactcaggct caattcctga
    21841 aaaataatcg caattcctaa ctggcgtgaa tgctgcggat tattaatggt gatagtttca
    21901 ccacgtagtg ttatcgttcc ctgggttggc ggataaacac cggccagtat tttcattaat
    21961 gtcgatttac cggcaccatt ctcgcctaat aaagtatgca cccgcccttt acgtaaagag
    22021 agggtaatat cttttaatgc ttgaacatga ccaaatgtct tcgcaatatg cgaaacatca
    22081 agaataacat ccgtcataat gacctcggtg tcacaacaaa acgccaataa cgttttgttg
    22141 tgaggtgaag atgtttacta tttacctgta taaacgccgg gttgtaccgg gatcattttt
    22201 tctaccggtt taccattgag gtgatcaaca gcggcatcaa tggctttagc acccatttga
    22261 tcgggatatt gccgaatcac ggccgcaaac gttttttcac tgtcaacggc atcacgcgct
    22321 tctttcatgc catcaaaacc aatgattttg atgttgtcgt tatgcgcaga ttttgctgcg
    22381 ataaccgctg ccagcgccgc atcatcacca aagccaaaca gaccattcag gttggcattt
    22441 gcctgcataa tgttttgcgc agtcgttaac gcttcagccc gcgtaatact aggctgttca
    22501 gcaacaattt taatatccgg tgtatcgctt aaccctttct taaaaccatc aacgcgcgca
    22561 accaccgatt gtagtgccgg ataagaaata atggctacag tacctttacc attcaaaagt
    22621 ttgcccatta acttgcctgc ttcaacgcct cccgcatagt tatcggtcgc aacatgcgat
    22681 acgaccggaa cgccttccgc agcgacgtcg acggtgataa cgggaatacc ggccttatcg
    22741 gctttaatta tcgccgcctg gacgcctttc gaatcgaccg gagagataat aatggcatcc
    22801 acttttttag taataaaatc ctcaacatca gaaagttgtt tgtttaaatc ctgattggca
    22861 aatag
//
LOCUS       DAZPEB010000047        22768 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZPEB010000047 DAZPEB010000000
VERSION     DAZPEB010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22768
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.048"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            89..718
                     /locus_tag="ACHURJ_001619"
                     /pseudo
     CDS             89..718
                     /locus_tag="ACHURJ_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            821..1324
                     /locus_tag="ACHURJ_001620"
                     /pseudo
     CDS             821..1324
                     /locus_tag="ACHURJ_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
     gene            complement(1391..2848)
                     /gene="nuoN"
                     /locus_tag="ACHURJ_001621"
     CDS             complement(1391..2848)
                     /gene="nuoN"
                     /locus_tag="ACHURJ_001621"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFR1280611.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQSLISIVRLAMPLM"
     gene            complement(2855..4384)
                     /gene="nuoM"
                     /locus_tag="ACHURJ_001622"
     CDS             complement(2855..4384)
                     /gene="nuoM"
                     /locus_tag="ACHURJ_001622"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFR1280612.1"
                     /translation="MLLPWLILIPFIGGFLCWQTEHFDVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4548..6389)
                     /gene="nuoL"
                     /locus_tag="ACHURJ_001623"
     CDS             complement(4548..6389)
                     /gene="nuoL"
                     /locus_tag="ACHURJ_001623"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFR1280613.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEILHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYFCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQSVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWQGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6386..6688)
                     /gene="nuoK"
                     /locus_tag="ACHURJ_001624"
     CDS             complement(6386..6688)
                     /gene="nuoK"
                     /locus_tag="ACHURJ_001624"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFR1280614.1"
                     /translation="MIPLQHGLILSAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6685..7239)
                     /gene="nuoJ"
                     /locus_tag="ACHURJ_001625"
     CDS             complement(6685..7239)
                     /gene="nuoJ"
                     /locus_tag="ACHURJ_001625"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFR1280615.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7251..7793)
                     /gene="nuoI"
                     /locus_tag="ACHURJ_001626"
     CDS             complement(7251..7793)
                     /gene="nuoI"
                     /locus_tag="ACHURJ_001626"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFR1280616.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7808..8785)
                     /gene="nuoH"
                     /locus_tag="ACHURJ_001627"
     CDS             complement(7808..8785)
                     /gene="nuoH"
                     /locus_tag="ACHURJ_001627"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFR1280617.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALIVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8782..11508)
                     /gene="nuoG"
                     /locus_tag="ACHURJ_001628"
     CDS             complement(8782..11508)
                     /gene="nuoG"
                     /locus_tag="ACHURJ_001628"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFR1280618.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSLE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11561..12898)
                     /gene="nuoF"
                     /locus_tag="ACHURJ_001629"
     CDS             complement(11561..12898)
                     /gene="nuoF"
                     /locus_tag="ACHURJ_001629"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFR1280619.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12895..13395)
                     /gene="nuoE"
                     /locus_tag="ACHURJ_001630"
     CDS             complement(12895..13395)
                     /gene="nuoE"
                     /locus_tag="ACHURJ_001630"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFR1280620.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13398..15200)
                     /gene="nuoC"
                     /locus_tag="ACHURJ_001631"
     CDS             complement(13398..15200)
                     /gene="nuoC"
                     /locus_tag="ACHURJ_001631"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFR1280621.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15294..15956)
                     /gene="nuoB"
                     /locus_tag="ACHURJ_001632"
     CDS             complement(15294..15956)
                     /gene="nuoB"
                     /locus_tag="ACHURJ_001632"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFR1280622.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15972..16415)
                     /gene="nuoA"
                     /locus_tag="ACHURJ_001633"
     CDS             complement(15972..16415)
                     /gene="nuoA"
                     /locus_tag="ACHURJ_001633"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFR1280623.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17046..17984)
                     /gene="lrhA"
                     /locus_tag="ACHURJ_001634"
     CDS             complement(17046..17984)
                     /gene="lrhA"
                     /locus_tag="ACHURJ_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFR1280624.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            18903..20120
                     /gene="alaA"
                     /locus_tag="ACHURJ_001635"
     CDS             18903..20120
                     /gene="alaA"
                     /locus_tag="ACHURJ_001635"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFR1280625.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20204..20802
                     /gene="yfbR"
                     /locus_tag="ACHURJ_001636"
                     /pseudo
     CDS             20204..20802
                     /gene="yfbR"
                     /locus_tag="ACHURJ_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
     gene            complement(20861..22693)
                     /locus_tag="ACHURJ_001637"
     CDS             complement(20861..22693)
                     /locus_tag="ACHURJ_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFR1280626.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPVGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
BASE COUNT         5844 a         6273 c         5696 g         4955 t
ORIGIN      
        1 tgtatagatc taacaaccgt aataaaacaa atactcataa ctcattataa ataatgataa
       61 aataacgtct tttattgata atttatatat gggaaaaatt ccttttgtta tattgtttct
      121 gatttcactc cctctgatta tttttaccaa ccctgggtca ctgcattacc accaacgccc
      181 cgacacgtca gtcctgagca attagaaaaa acagttcgtt atcttacaca aactgtgcat
      241 ccacgtaatg ccgacaatat tgataatctg aataggtccg tagagtatat aaaagagttc
      301 tttgtcagta gcggtgccag agttaccgcg cagggcgtcc ccgattggct attacgactc
      361 tgcgcctggt agccaggatt acctttaccc gcaatgtcct ggctttatcc cgatcggggg
      421 gactttattg cctggtcggc agaatacagg atatcaacgc cgttcgtcag gtaaaaacgg
      481 cattgttatc atcccgggat ttatctgttt attctatgaa tgccccatgg tttattcccg
      541 gtattgattt ctctgaccac ctgaattatt ggcaacacga tattcccgcc gtaatgatta
      601 ctgacaccgc tttttatcgt aataaacaat accacttgcc cggtgataca gcagacagat
      661 tgaattatca gaaaatggct cagatggtgt tataaccttg ttacacaaca gtaaataata
      721 aaaatttctc gaggcgggtc cagaaacagc gacatgtaca ttttattttc cctcgtctgt
      781 tttaagatgc gaaacataaa attcaatgga ggaaaaataa atgggaatga ttggttattt
      841 tgcagaaatt gactcagaaa agattaacca atctgcttga gtctactgaa aaacctttga
      901 tggataatat acatgacacg ctttcaggat tacgccgact ggatattgat aaacgctggg
      961 attttttaca cttttgggct gacaggcacc cctgcttttg atcccgcgaa gaatgatccg
     1021 ctgagtcgtg ccgtgcttgg tgaacacagc ctcgaagatg gcatagatgg cttcctggga
     1081 ttgacctgga atcaggaatt agccgcgaca atcgaccgac tggaatcact ggatcgaagt
     1141 aagctgcgta aacagttttc cattaagcga ctatgaaatg gagatttatc cgggagtgac
     1201 cttttcagaa gagttagagg ggcaactctt tgcctcgatc atgcttgata tggaaaagct
     1261 gattagcgcg tatcgacgta tgctgcgaca agggaatcat gcgctgacgg ttattgttgg
     1321 gtgatggtgt cagagcgcca aaaaaaaccg ccgaacatgt cggcggtttt ctgacttaca
     1381 aactaacaga ttacatcagc ggcattgcta accgcacaat actaatcagc gattgtggcc
     1441 atacacccag caccagtacc aacagtgcag aaatcagcac cacgataccg cccgcgctgt
     1501 actgccagtt tgacggtgca tcgcgacccg gttgttccgg tgcgtgcagg tacaggctca
     1561 ccgccacgcg caggtagtag tagaggccga ttgccgaacc gacaaccacg gcacccacca
     1621 gccaccacaa gtgtgcctgg acaccgaccg ccagcacgta gaacttaccg ataaagccca
     1681 gcgtcatcgg gataccggcc agagacagca tcatcaccgt catcactgcc gcgaggatcg
     1741 gacgatgcca gaacagaccg cggtaagaga acagggaatc agcatccggg ccacgatacg
     1801 ggctggacat caggctgacc acgccgaacg cgccgaggct gctgaacaga taaccggcca
     1861 ggtaaacccc taccgcttcc atcgacatct cgccggtttg cagcgcaatc agcgccacca
     1921 gcagatagcc gaggtgagag atagatgagt aaccgagcag acgtttgatg ttggtctggc
     1981 tcaacgccat caggttaccg aagatgatgg aggcaaaggc gataatcgcc agtactacgc
     2041 gaatcgcttc gctgtcaccc accggtgcat agaggaacag acgcatcacc acaccgaaga
     2101 tagcgatttt gctcgccgtc gccaggaaag tggaaaccgg cgcaggcgcg ccctggtata
     2161 cgtctggcgt ccacaggtgg aacggcacca gagagagttt gaagccgagg ccaacaatca
     2221 tcatgccgaa acctgccagc aacagcggct cgttgagcat accgtcgccg aggtttttac
     2281 ccagcgcgac aaacgacagg tcgccagact gcgcatacac cagcgccata ccaaacagca
     2341 ggaaagaaga cgctgcggca gaaaggatgg tgtatttgat actggcttcc agcgaacgtt
     2401 tctggcggaa agcgtaaccg accaggccaa acagcggcaa agagatcagt tcaataccga
     2461 ggaacagaga cgccagatgg tttgcattcg ccagcaggat cccgcccagc gcggcaatta
     2521 acaccaacag gtagaactca tccttgttgt cgttatagcc ttcaagccac gggtaggcga
     2581 aagtacaggt ggcgaggctc gccaacaata ccagcccggt gtaaagcatg gcgaaaccat
     2641 caacgcgcat cagcggcgta acgtccatag cgcccgcctg gccaacaaac cagagcgaaa
     2701 ccagcgccgc gttaagccca ataaccgaga gcgtagcgtt gaggaaatga ttgcgtcgcc
     2761 acgcaatgga gagcatcaca accaccaccg ttaagccgac gatcagcaac ggtagcagtg
     2821 cgatcaggtt ttgtggagtt attgtcatgg cgatttacgg ccttgtagta gtaacggaat
     2881 taacaaacca ctgctggata ttgccaatcg cggagtgcga ggtatccaga atcggctgcg
     2941 gatagaagcc cagcagtacc agcagcacca ccagcaacag gatcataaac agctcacgca
     3001 gcgacatccc tggcagttcc tggctggcaa tctggctttt cgctttaccg aagtaagcgc
     3061 gatgcaacat cgccagcgaa taaacagatg caaagaccag cccaaaggta gagataacgg
     3121 taatcaccgg gacaacctgg aagctgccga acagaatcat aaattcgccg acgaagttac
     3181 cggtgccagg catcccaagc gttgccaccg caaagaacag cgacaatgct ggcagccatt
     3241 tcatcttgct ccacagaccg cccatcatgc gcatgtcgcg ggtatggata cgttcataaa
     3301 gctgaccaca aagaataaac agacccgccg ccgacaaacc gtgcgcaatc atctggatta
     3361 ccgcgccctg gtaggccaac tggctgccgg tgtagatagc aatcagcacg aagcccatgt
     3421 gggaaaccga ggtgtaggcg atcagacgtt tgatatcggt ctgggcgaag gccatccacg
     3481 caccgtagaa gatgccgata acacccagcc acatagcaat tggcgcgaac tctgccgacg
     3541 cgttcgggaa cagcggcagg gagaaacgca gcaaaccgta agcggcagtt ttcagcaaga
     3601 tccccgcgag atcaacggaa ccggcggtcg gagcctggga gtgcgcatcc ggcagccagc
     3661 catgcagcgg aaccaccggc attttgactg cgaaggcgat gaagaagccc agcattaaca
     3721 ggtattccac accactggac attggcgtat tcagcagctc ttcatagttg aaggtccaga
     3781 cgccggtcgc attgtagtga acaaaaacca gcgccaggat ggcaatcaac atcaccaggc
     3841 cactcgcctg ggtgtaaatg aagaacttgg ttgccgccgt gatacgcgtt ttaccgtcag
     3901 aggctttatg cccccacagt gcgatcagga agtacatcgg caccagcatc atttcccaga
     3961 agaagaagaa caggaacatg tcgatggcaa ggaacacgcc gataacgccg cccaggatcc
     4021 acatcaggtt gaggtggaag aagccctgat atttttcgat ctctttccac gaacagagta
     4081 ccgccagcac accgagcaga ccggtcagca cgaccatcag cagcgacaga ccgtcaatgg
     4141 cgagatgaat agagatacca aaacgtggga tccacggcat gtcgaattca gactgccact
     4201 gcggaattcc ggcggattgc gtcagtgaat aaccgccctg caaccacagt tgcagcgaca
     4261 gcgccagcgt caatcccatg gtgatcagcg cgatccagcg tggcaccttg acgtcaaagt
     4321 gttcggtctg ccagcacagg aagccgccaa taaagggaat taatattagc cagggtagta
     4381 acatggcgat ctttattcct tgtaaaagtc ccgtcaggac cggattttca acgaattctc
     4441 acgacagaaa attctcttcg gactgggggt agtgtcggat gcgccgcgaa ggcagcttat
     4501 ccgacctacg ttcttatatg cccaggggca atcccacaat ccttaactca acgcagtacc
     4561 atcaacagtg ccagcaccac gaccgcaccg atgctcatgg atgccacata ccagcgcaga
     4621 taaccgttct cgcttaacag caggccttta cctgcaaagc gggaaagaac cgccggaatg
     4681 ttcatcattg agttcagcgg atcgcgtttc agcaaccagg caatacccag aaacggcttg
     4741 acgaacactt tgtcgtacag ccagtcaaag ccccaggcgt tgtaccacca ggtgcccagc
     4801 agacggcccg gcgcactgtt ggcaatggag gtcaccagag tacgtttacc ctgccacagc
     4861 caggctgcca gcagaatgcc gaccaccgcg accacgccag aggtaatttc cagggtcaac
     4921 atgctgccgt gcgccagttc cgtcgtttgc ggaagcacgc tctgcagcgg cggtacaatc
     4981 agtgcgccaa cgaaggtgga aaggatcagc agcacaatca gcggcaggct gtgagttacc
     5041 cctttcacgg cgtgagcgtg aatttgttct tttccgtgga agacgatgaa aatcatacgg
     5101 aaggtgtaga gcgaggtcat aaacgcaccg accagacctg ccaccatcag attgatatga
     5161 ccattcgcca tcgcacccgc gaggatctca tccttactga agaagcccgc agtgaccagc
     5221 ggtagtgccg acagtgctgc gccgcccacc aggaagcaga aataaaccag cggaatagat
     5281 ttacgcagac cgcccatctt gaagatgttc tgttcgtgat ggcaggccag aatgacggaa
     5341 ccggatgcca ggaacagcag cgctttaaag aacgcgtgag tcatcaagtg gaaaatcgcc
     5401 gcatcccatg cctgcacgcc aagcgcgagg aacatgtagc caatctggct catggtagag
     5461 taagcgagaa cacgtttgat gtcggtctgt accagcgcgg caaaaccggc cagcagcagc
     5521 gtaaccgccc cgacaatgcc caccagatgc agaatttccg gcgtcatcag gaacaggccg
     5581 tgggtacggg cgatcaggta gacacccgcg gttaccatgg ttgcggcgtg gatcagcgcg
     5641 gagacaggcg tcgggcccgc catcgcgtcg gcaagccatg tctgcaacgg caactgcgca
     5701 gatttaccga ccgcaccgcc cagcagcatc agcgtcgccc acatcagcat gttattgccg
     5761 tcagcaaagt gcgctggtgc cagttccacc atttcgcgga agttcagggt gcccagttcg
     5821 ttgtaaagaa tgaacagcgc gaaagcgagg aacacgtcac ccacacgggt cacgacgaac
     5881 gctttcattg ccgctgcgcc attcttcgga tcggtgtaat agaacccgat cagcagatag
     5941 gagcacaggc ccacgccttc ccagccgagg tacatcagca gcaggttatc ggcgagaacc
     6001 agaactacca tgctggcgat gaacaggttg gtgtaggcga agaagcgaga gtagccctct
     6061 tcaccgcgca tgtaccagga ggcgtacatg tgaataagga aacccacacc agtgaccacc
     6121 gagagcatgg tcagcgacag gccgtccagc accaggttaa aaccgatgtt aaagtcgcct
     6181 accgacatcc acgtccacag cggctggctg tatgcctgct cgccgttagc gaagaaatca
     6241 acgccgataa aggcggttac cagcgccgcc aggcccacag agccaacgcc gacgatcgcc
     6301 gaaacgtttt cagaccagcg cccacgggag aacgccagca ggacgaagcc aatcaatggc
     6361 aaaataatgg ttaaggcaag catgttcatc cgcgcatctc acttactgaa tcgatgttca
     6421 ggttctggcg acgacggtga agttgcagca gcagcgcaag gccgatactc gcttctgccg
     6481 ccgcgaggct gatggcgaga atgtacatca cctgaccgtc ggtctgaccc cagtagcttc
     6541 cggcgaccac aaaggccagc gcggaggcgt taatcatgat ttccagacca atcagcataa
     6601 acagcagatt gcgacggata accagaccgg ttaagccaag aacgaataag attgccgaga
     6661 ggatcagtcc atgttgtaag gggatcatgc gtgctcctcc gtttttcttt tcgcgctgtc
     6721 gtctttacga ttgctcagca cttcacccgc acgctcttca cgaccgacgt ggaaggccac
     6781 aaccagaccc gcgagcagca gcatagaagc cagttccacc gccagtacgt aaggcccgaa
     6841 cagcgtaata cccactgctt tagcactgat tggcgtaccg tcgataccct gatcattaac
     6901 accaaggatg gcgtaaacaa tcaccaccag catgatggcc gacaaaattg ccggaccaat
     6961 ccacacctgc ggtttcagcc actggcgttc ctgttcgatt tctgaaccgc ccaggttgag
     7021 catcatcacc acgaacacga acagcaccat aatggcaccc gcgtagacga taatttccag
     7081 cgcaccggcg aagtaagcgc ccagtgagaa gaacaccccg gaaatcgcca gcagcgaaat
     7141 aatcaggtac agcagtgcgt gtaccggatt ggtatgggtg atcactcgca aggtcgcaag
     7201 tatggctatc aggccacaga tataaaaagc gaactccatt gcccctctcc ttacggtaac
     7261 aggctcttga cgtcgatagg cttggcttcg ttctctgctt cgcccttatc tttgccgtcg
     7321 attgccatac ctgccatccg gtagaagtta tattccgggt atttgcccgg accggagatc
     7381 agcagatcct ctttttcgta aaccagatcc tggcgcttgt attcccccat ttcgaaatcc
     7441 ggggttaact gaatcgccgt ggtcggacag gcttcttcgc acagaccaca gaaaatgcag
     7501 cgtgagaagt tgatgcggaa aaattccggg taccagcgac cgtctttggt ttctgctttt
     7561 tgcagcgaga tacagccgac cgggcaggct accgcgcaga ggttacaggc tacgcaacgc
     7621 tcttcgccgt ccgggtcgcg ggtcagaacg atacgaccac gataacgggg cggcagatag
     7681 accggctctt ccgggtacat tcgcgtttcg cgtttggcga acgcgtgcag gccgatcatc
     7741 cagatactac gaacctgggt gccgaaacct actaacaatt cttttaaggt catggtctta
     7801 ttgcccctta ttgcgcctgc cagagaatga cagccgccgt taccagcaag ttgatcagcg
     7861 tcagcggcag gcagattttc cagccgaagg acattacctg gtcataacgc ggacgcggta
     7921 acgacgcacg aatcaaaatg aacatcatca taaagaacgc ggttttcaac gcgaaccaga
     7981 tgaatggcgg taacaacggg ccttgccagc caccgaagaa cagcgtcaca atcaatgcag
     8041 agatggtcac aatcccgatg tactcaccca cgaagaacag accgaacttc ataccggaat
     8101 attcaatgtg gtaaccatcc gccagttcct gctcggcttc cggctggtca aacgggtgac
     8161 ggtgacatac cgccacgccc gcgatggcaa aggtaataaa accaaagaat tgcgggataa
     8221 cgttccacac atgcgcctgg ctgttgacga tgtcggtcat gttgaatgaa ccggcctgcg
     8281 ccaccacgcc catcagggaa agcccgagga acacttcgta gctcagggtc tgcgcagaag
     8341 cacgcatcgc acccagcaac gagtatttgt tgttacttga ccagcccgca aacagcaccg
     8401 cgtaaaccgc cagacctgcc atcatcagga agaacaaaat cccgatgttc aggtcggcaa
     8461 ccacccaacc cggactgact ggcacaatcg caaaggccag cagcagcgag gtaaaggcaa
     8521 tcatcggtgc cagggtaaag atgacgcgat ccgagaattt cgggatccag tcttctttaa
     8581 agaacatttt gatcatgtcc gcaaccagct ggagcgaacc gccccagcca acacggttag
     8641 gtccataacg gttctggaac agacccagca ggcgacgttc gccaaagctc atgaatgccc
     8701 cacaggtgac aaccaccagc aggatcacca ccgctttgag gatggtcagc aggatctcaa
     8761 tcagttccgg tgatatccaa ctcattgttg tgcctccttg agatcctcaa gatgcgcgcc
     8821 agccagcacc ggagcaatgc cggacatacc catcggcaag cccacctgcc ctgccgtcag
     8881 tccttcggcg atttcaaccg gcagcgtgac cgtgttgcca tcgtaactaa aggagacgcg
     8941 tgtacctgcg ttcacaccca acttcgcggc atccgctggg ttgagtttga tgtacggctg
     9001 cggcatacgg ctctggaaga ccggagcacg ctgtgacaat tcatcgctgc caaacaggtg
     9061 gtaatacggc gcaatacgcc atttcccgtc ctgcggctgg aagcgtgccg gtacgctggt
     9121 gaagtaatcc agaccattct cgctggtttc aaacagacgc acgcccggat cgccaaagcg
     9181 cagtttgccg cccacttcgt cctggaattt gttccacgcc tgcggggagt tccagcccgg
     9241 tgcccaggca aacggcactt gcgaacggtg cgcggtcggc tggttgttac cttccatcga
     9301 gaaggtgaac atggtgtcaa tatcctgcgg ctgacgcggc tcatgaacgc tgatattggc
     9361 gcgcatggcg gtacgaccgc tgtaacggtg cggttcacgg gccagtttct gcccacgaat
     9421 acggaatgtc gcatccggcg cagcatcttt gatacctgcc agttccggga ttttcgccac
     9481 aacagcgtca atcacatggt cgagctgcgt ccagtccact tcacggctca gcagggtgct
     9541 gtgcagcgag tgcagccagc gccagctttc cagcatgaca gttttgctgt cgtaataagc
     9601 cggatcgtaa acctggaaga aacgttgggc gcgaccttca ttattaatta ccgtaccgtc
     9661 gctttcagca aagctggcgg cggaaagtac cagatgggcg ttttccatga tcgctgtacg
     9721 ctgatggtca accaccatca ccagcggtgc tttagccagc gcagcattca cgcgggtagc
     9781 agaagcgtga cgatgcagat cgttttccaa caccaccacc gcgtcggcgc gtccggtttc
     9841 cagttcggtt aacgcttctt caagcgaacc gccacccata atgcccagcc ccatgctgtt
     9901 gacggaacgg gcaatcatgg tgataccgac gtcagcgccg cgacctttca gggctttcgc
     9961 gacgttagcc gccgcctgaa tcacctctaa gctaccggcg ttcgtcccgg agataatcaa
    10021 cggtttcttc gcacctgcca gtgcctgcac gatgacgtcg attttgcttt gcagctcagg
    10081 ttcgataccg tcaaccgctg gtgcagagtt atccagcgca tgggcgatgg caaaacctaa
    10141 acgcgcctga tcttcaaccg gtgcgcggta agtccacgcc gcgatatcat ccaaacgggt
    10201 gtcatcaacg ttggtaacaa acagcggatg cttcgcacgt tgaccgatgt taaggattgc
    10261 cgcaatctgc cagtcagcca ctttctgtgc tgccgccatt tcgcgcgctt tacctttcac
    10321 cgcctgacgc actgccagcg cgacgcgcgc gccggtctgg gtaacgtctt cgcccagcac
    10381 cagtaccgca tcgtaagatt cgatttcgcg cagagccgga gtataaatgc cgccttcacg
    10441 cagcactttc agcgccagtt gcagacgttc ctgctcaccg tgagcgatac cggtgtagaa
    10501 gttttcttcg cccaccagtt cacgcagtgc aaagttgctt tccacgctgg cacgcggaga
    10561 accaataccg atcactttct tcgactgacg cagaatatct gccgcgccct gcattgcctg
    10621 ttcggcgttg agggtaatga aatcatcgcc acgacgctgt actggctgac gcggacgatc
    10681 cttcaggttg acgtaaccgt aaccgaaacg accacggtcg cagaggaagt agtggtttac
    10741 cgtaccgttg taacggtttt cgatacgacg cagttcgccg taacgttcac cggggctgat
    10801 gttacagccg atggaacatt gctggcagat gctcggcgca aactgcatat cccatttacg
    10861 gttgtaacgc tcggagtgcg ttttgtcggt aaatacgccg gtcgggcaaa tttcgaccag
    10921 gttaccggaa aattcgcttt ccagcgtgcc gtcttccggg cgaccgaagt agacgttgtc
    10981 gtgcgcgcca taaacgccca gatctgtacc gtcagcgtaa tctttgtagt aacgcacaca
    11041 gcggtagcag gcgatgcagc ggttcatttc gtgagatatg aatggcccca aatcctgatt
    11101 acggtgggta cgtttggtga aacggtagcg acggaagctg tgtccggtca tcacggtcat
    11161 atcctgaaga tggcagttac cgccttcttc acataccgga cagtcgtgcg ggtggttggt
    11221 cattaaccac tcgaccacac tttcacggaa ctgtttcgct tcttcgtcgt caatggaaat
    11281 aaaggtgcca tcggaagccg gtgtcataca ggacatcacc aggcgaccac gcgtgtcttc
    11341 cgcgttttgg tattgcttca ccgcacactg gcggcaagca ccgacgcttc ccagcgccgg
    11401 atgccagcaa aagtaaggaa tatcaaggcc cagagacaga caagcttcca gcaggttgtc
    11461 cgctccgttg acctcgtatt ctttgccgtc tacatgaatt gtagccatta gcatgcttcc
    11521 agttttctcg gtcagggacc gagcgttaat cgaaattcgg ttaccagcgc tctttcagca
    11581 ggttcggctg aatcccatta atcaaatggg tattgctgaa cggctgtttg attcccgcct
    11641 caaattcttc gcggaaatat ttgatggcgc tctgtaacgg ctccactgca ccaggtgcgt
    11701 gggcacagaa agttttaccc gggcctaaga atcgacacag ttgctcaagt gtttcaatat
    11761 cgcccggctg gccttcgcca cgctccagcg cacgcagaat tttcacgctc cacggcagac
    11821 cgtcgcggca cggcgtacac cagccgcagg actcacgggc gaaaaactct tccaggttac
    11881 gcaccagcga caccatgttg atctcatggt caaccgccat cgccagcgcc gtacccagac
    11941 ggctgcccgc tttaccgata ctttcgaatt ccatcggcag atcaaggtgc gcttcggtca
    12001 ggaagtcagt ccccgcgccg cctggctgcc aggctttaaa tttcagacca tcacgcatac
    12061 caccggcgta atcttcgagg atctcgcgtg cggtggtgcc gaacggcagt tcccacagtc
    12121 ccggattttt cacccgaccg gagaagccca tcagcttggt gccagcatct ttacttttcg
    12181 agatgttctg ataccactcc acgccgttag cgaggatcgc cggaacgtta cacagggttt
    12241 cgacgttgtt gacacaggtc ggtttgcccc atacgccgga ggttgccggg aaggggggtt
    12301 tcgagcgtgg gttagcacga cgcccttcta gggagttgat taacgctgtt tcttccccgc
    12361 agatgtagcg ccctgccccg gtatggacga acagttcgaa gtcgaaacct gttcccataa
    12421 tgtttttgcc aagcaagccc gcttcagtgg cttcggcaat ggcgcggcgc agatttactg
    12481 ccgcttcgat atattcgcca cgcaggaaga tgtagccacg gtaagctttc agcgcaaacg
    12541 cggagatgag cataccttcc accagcaggt gcggcagttg ctccatcaac aggcggtctt
    12601 tataggtgcc cggctccatt tcatcggcgt tacacagcag gtaacggatg ttcatggatt
    12661 cgtctttcgg catcaggctc cacttcaggc cagtagaaaa gcccgctccg ccgcgccctt
    12721 tcagaccagc gtcttttacc tgattaacga tttcgtccgg agacagcccg gtcagcgcct
    12781 tacgcgcccc ttcgtaaccg tttttgctgc ggtattcgtc cagccacact ggctgtttgt
    12841 catcgcgcag acgccaggtc agcggatgcg tttcgggagt acggataatg tttttcattt
    12901 ataccgctcc agcagttcag ggatcgcttc cggggtcaga tgcgcgtgag tgtcctcatc
    12961 gatcatcatg tttggccctt tatcacagtt ccccaggcag caagttggca gcagcgtaaa
    13021 gcggccatca aatgtcgttt gccctggttt gatgttcagc tttttctcga gcgccgcctg
    13081 aataccctga tacccgttga tatgacagac cacgctgtcg caataacgga tcacatggcg
    13141 accaaccggc tggcggaaga tctgactgta gaacgttgcc acaccttcga cgtcgcttgc
    13201 cggaataccc agcacatcgg cgatcgcgtg gatcgcacca tccggcaccc agccacgctg
    13261 cttctgaacg attttcagcg cttcaatgga cgccgcacgc gggtcttcgt agtggtgcat
    13321 ctcgtgctca atcgcttcac gctctgccgc actcagctca aaagcctcgg tttgtggttg
    13381 ttgattctcg tgcataatta gcggtccaca tctgacataa caaaatcgat actgcccaga
    13441 taaacaatca ggtcagacac caggctgccg cggatcgccg ccggaatttg ctgcaaatgc
    13501 gcataactcg gcgtgcggat acgagtgcga taactcatgg tgctaccgtc gctggtcagg
    13561 tagtaactgt tgatcccctt ggtcgcctca atcatctgga aagattcatt ggcaggcatc
    13621 accggccccc acgacacttg caggaagtgg gtgatcaggg tttcgatatg ttgcagcgtg
    13681 cgctctttcg gcggcggcgt ggtcagcggg tgatccgctt tgaacgggcc ttccggcatg
    13741 ttgttgaggc actgctcaag aatgcgcaga ctctggcgca gctcttccac tttcagcatt
    13801 acgcgggtgt agcagtcaga aacaccacca ccaaccggga tttcaaagtc gaagttttca
    13861 tagccagaat aaggacgcgc cttacgcacg tcgaagtcga tcccggtagc acgcaggccc
    13921 gcgccagtgg tgccccactc cagcgcctct ttcgcgccat aggcggcaac gccctgggaa
    13981 cgacctttca gaatggtatt ttgcagcgcc gctttctcgt aagacgccag acgtttcggc
    14041 atccagtcga ggaactcacg cagcaggcga tcccagccgc gcggcaggtc gtgcgctacg
    14101 ccgccaatac ggaaccacgc cgggtgcata cggaaacccg tgatcgcttc caccagatcg
    14161 taaattttct gacgatcggt aaaggcgaag aacacgggcg tcattgcgcc gacgtcctga
    14221 ataaaggtcg agatgtacag caggtggctg ttgatacgga acagttcgga gagcataacg
    14281 cgaatgacgt taacgcgatc cggcacggtg atcccggcca gtttctctac cgccagcacg
    14341 taaggcattt cgttaacgca gccgccgagg tattcaatac ggtcggtata cggaatgtag
    14401 ctgtgccagg actggcgctc gcccattttc tccgcaccac ggtggtggta gccgatgtct
    14461 ggtacgcagt cgacaatctc ttcgccgtcg agttgtaaaa cgatacggaa agccccgtgc
    14521 gccgacgggt ggttcggacc gaggttgagg aacatgaagt cctcgttttc ggtgccgcgc
    14581 ttcatccccc actcttccgg tttgaaggtc agggcttcca tctccagatc ctgtttggct
    14641 ttggtcagct caaacggcga gaattcggta gcgcgcgccg ggtaatcttt acgcagcggg
    14701 tgacctttcc aggtttgcgg catcatgatg cggcgcaggt tcgggtgacc gtcgaaagta
    14761 atgccaaaca gatcccaggt ttcacgctca taccagttag cgttcgggaa cagtttggtg
    14821 aaggtcggta cgtgcaggtc gttttctgcc agcgccacct tcagcatgat gtcgcggtta
    14881 cgatcgatag aaatcagatg gtagaaaacg gaaaaatccg cggcaggtaa cccttcgcgg
    14941 tgtgtgcgca gacgttcgtc catgccgtgt aagtcaaaca gcatgacgta aggtttcggc
    15001 agtttcttta agaaatcgcc aacttccagt aattgttcac gcttgatcca cacaacggga
    15061 accccggtgc gagtcgcctg aacagtaaag gcatccggcc caaaacggtt gcgcagttcg
    15121 ccaattaccg gatcatcaag atgatcgcgg gtctgccagg cgggttcttg cgcggttaag
    15181 tcggtcatat tgttcaccat tgcaaatggt ccgtggtgac tgtcggcgca gtaagcttcg
    15241 cgaaatagat ttaggaataa gcgaagaaaa atccctttgc cgacaggcgc aaattaaatc
    15301 tcgtcaggtg tacgcaggtt ggttacggca atgcgttcac cgcgcttgcg ttcgcgctct
    15361 gattgcatat tggcgcgata aacgccctga tcgccaacca cccaggagag cggacgacgt
    15421 tctttgccga tagattcctg caacagcatc agtgcctgca tgtacgcttc aggacgcggc
    15481 gggcagcccg ggatatacac atcaaccggg atgaatttat cgacgccctg cacaacggaa
    15541 taaatatcgt acataccacc agagttggca caggcaccca ttgagataac ccattttggt
    15601 tccagcatct ggtcatacag acgctgaata accggtgcca ttttggtaaa gcaggttcct
    15661 gcaaccacca tcaggtcagc ctgacgcggc gaagcacgca atacttctgc gccaaaacgc
    15721 gccacgtcat gcaccgcggt aaacgaagtc accatctcaa cgtaacagca ggaaagaccg
    15781 aagttatacg gccaaattga gtttttacga ccccagttaa ccatgtcatt gagcttgccc
    15841 ataaacacgt ttttgttaac ttcttgctcc agagggtcgg ttacgatctc ctgcttttgc
    15901 agggggtaac ggtcgttctc accgttggga tctatgcggg tgagcgtata atccatctta
    15961 atgcctcgcg gttagcgttg acgattagcg atactgttcg tttccgggtt catacgctcg
    16021 cggcgtgaac gcgcgggcgt ccagtccagc gcgccaatac gcaccagata aaccagacct
    16081 gccagtaaca caaaaataaa aattgcagct tccacaaagc ctacccagcc gctttcgcgg
    16141 atagaggttg accatgcgaa cagatacagc gcttcaacgt cgaagataac gaagaacatg
    16201 gccaccagat aaaacttggc ggacaggcgt aagcgggcgg agccgaccga gtcgataccg
    16261 gattcaaacg gcacgttttt cgacctcgcg cgtgcgcgac cgcctaaaaa ccaaccacct
    16321 accagcatta ggcaacacag gccaatggca acgataagaa agatagcgaa tgcccagtga
    16381 tgagcgatga cttcagtgga tgttgacata ctcattgctt actcatcaaa agtagcgcca
    16441 gattcactgc tcttcacggc agatggacgc cacatcgatt catggggagg aataaaaaaa
    16501 accttacaat cactgtagaa attcttttat acagctaatt gatgtggtct tttactcctt
    16561 tctataacct tttgtcaact ttaacaaaag tttcttcaca ttagtttaca taatatcaac
    16621 accattagca tttaatgccc tttcacccca gatccttgac gactccagga taattagatg
    16681 ttgttgaatc gtgtccgttg tgaagcaatg gaaaaaatac gggtctattt tgacaggaat
    16741 ttgtgtcgat tcctcccccc aaaagagagt attttcttga tctgtgacac gcttttgtca
    16801 ttccataaca aaaacgcagc aacaaattta cgtatttttt aacatcattg tagcaggtga
    16861 tttttttcag gcgattattt gtgcgttcgg gacgtgaatc tctggtgatt gaaaaatgaa
    16921 cagttttgta cgttctgcac tatgcgagga aggattttta ctaaaaaaaa gccgctgggg
    16981 tttaaaacac ccccagcggc tcgtttttta cactattgtc tcaggaatta tctatcgtcc
    17041 gtcgattact cgatatccct ttcaatcaac aatgaatcat ccccttccgg agcagacatt
    17101 gggctgtatt gccacggatt atggtagctc tccatcgcct gatagatcac ctgggccagc
    17161 tcattattac tggacgggtc atagcacagc aaatattcgg tatcaggtag cggcggtaag
    17221 ccatctactc cactcagcac acgcaggtcc gggctcatca tctcaaccgg ccttgccgtc
    17281 acgccaagac cggctttcac tgccgcacga acggccggaa gcgtcgaggc gacataagcc
    17341 agtcgccatg gaatatctgc tttattaagc gttgccagca ccatatcgcg aaacggactt
    17401 ggatcatcca gcaatacaag agggatcggc tcgccttttt gcaatacgta ttccgctgcg
    17461 cagtaccagt gtgttggcga ggtacgcagg ttcaatgcct taaacgctga aggacgatgg
    17521 gtggttacca tcaaatccac ttcctgggat tccagcattt ctgccatata ggcattacgt
    17581 ttaacgcgga catccagcgc taatttcgga taaaccgaac tcacgcgatt taacaggaaa
    17641 ggtaagatcg tatcggcaga ttcatctgaa gcaccgatag ttaacacgcc ctgaagatta
    17701 ctgaacatta atgatgagca ggcctcatca ttaaaacgca ggattttcct ggcgtaacca
    17761 agaagttgaa tgccatgttc agttaacagt ttgttgcgac cgtgacgagc gaacagttct
    17821 ttcccaacga gttgttccag acgctgcatt tgctgactta cggcggactg agtacgacac
    17881 acagcggcag ctgcggcagc aaaagtgttc agatcggcaa cagcaacaaa tgttctcagc
    17941 agatcgaggt cgaggttaat tatcggacga tttgcactta tcatatatta tcacttactg
    18001 gcggctcata ctgagctggt taatgctgtg cacacacaaa caagcaattc catttgtaat
    18061 gtgcctccct ggcagtttca tccggaaccc ggacgaaagt aaaaatgcat atgagttgca
    18121 ctaaaaaagc gactcacatt gttccgttat aatgcctgaa gtagatcaca gaatatatct
    18181 tcagggatcg catatctatt aagttactca ctcttttcta tttatgacat gcgcgtgttt
    18241 gtataaatgt aaatgtgagt ccttgttcca ctctcgtgca gcatcgctgg tcatacgcga
    18301 acacgtacaa acagcaatgg tgaggcggca tcaagagcag ggatccgtca tttatgcgag
    18361 cattttaccc caaaaacctt ttatttataa gggccattgc gaatcatctg atgcaaagtt
    18421 atgttatgtt aggcaaagta atcttctatt attaataagc acatcaaaac ctttttgaat
    18481 attaaataat aattaattag catcatcctc attcattaat tccgcttaac aatagtttca
    18541 caccttgccc cattacgaca cctcggaaat catcacgctg ttaaactctc cgtaatgcgg
    18601 aaatatcatc atcgcgtttc attagtgaat tcttaaatga ggcattttca cacaattatc
    18661 ttacagataa aaaaaccaga cttacaatta agaatcagaa caattcacta tataacattg
    18721 catgtaaagc atatacacct cattattttg tcatcattaa gttattaaca gcacaatcga
    18781 gccttcccct ctggcaaaat cttattctgc agaccttcaa aacaccgtcc tgggggagta
    18841 cattgttcta agctgacttc cacggcaggg agtggcgata acagcaaaaa aggtcaagat
    18901 tcatgtcccc cattgaaaaa tccagcaaat tagagaatgt ctgttatgac atccgtggtc
    18961 cggtgctgaa agaagcaaaa cgcctggaag aagaaggtaa caaggtactg aaactgaaca
    19021 tcggcaaccc agccccgttc ggttttgacg cgccagatga aatcctcgtt gacgtgatac
    19081 gcaacctgcc taccgctcaa gggtattgcg attccaaagg tctttactcc gcgcgtaaag
    19141 ccatcatgca gcactaccag gctcgtggca tgcgtgatgt taccgtggaa gatatttaca
    19201 tcggcaatgg tgtatcggag cttatcgttc aggcaatgca ggcattgctg aacagcgggg
    19261 acgaaatgtt ggttcctgca ccagattacc cactatggac cgcggcggtt tcgctttcca
    19321 gcggtaaagc ggtgcattat ctttgcgatg aatcctctga ctggttcccg gacctcgatg
    19381 atattcgcgc taaaattacg cctcgtacgc gtgggatcgt tattatcaac ccaaataacc
    19441 caaccggcgc ggtatattcc aaagagcttt taatggagat tgtggagatt gcacgtcagc
    19501 ataatctcat tatcttcgcc gatgaaattt atgacaaaat tctttacgac gacgctgagc
    19561 atcactcaat tgcgccgctg gcacctgacc tgctgaccat tacctttaac ggactgtcga
    19621 aaacgtaccg cgttgcaggc ttccgtcagg ggtggatggt gttgaacggg ccgaaaaaac
    19681 acgccaaagg ctacatcgaa ggtctggaaa tgctggcttc aatgcgcctg tgtgctaacg
    19741 ttcctgcgca acacgccatt cagaccgcgc tgggtggtta tcagagcatc agtgaattta
    19801 ttacccctgg cggtcgtctt tatgagcagc gtaaccgcgc gtgggaactg atcaacgata
    19861 ttccgggcgt ttcctgcgtg aaacctcgtg gtgcgctgta tatgttcccg aaaatcgacg
    19921 ccaaacgctt taacattcac gacgatcaga aaatggtgct ggatttcctg ttgcaggaaa
    19981 aagttctgtt ggtgcaaggg acggcattca actggccgtg gccggatcac ttccgcattg
    20041 tcacgctacc gcgtgtcgat gatatcgagc tgtctttgag caagttcgcg cgtttccttt
    20101 ctggttatca tcagctgtaa tcttaatttc actgccggag attgcatccg gcagcgttat
    20161 cccgccacaa tgacctgatg atgtcatcat acgtaaggtc actatgaaac agagccattt
    20221 ttttgcccat ctctcccgcc tgaaactcat taaccgctgg ccgctaatgc gcaacgtgct
    20281 gacggaaaat gtgtccgaac acagtttgca ggtagcgatg gtcgcccatt cgctggcagc
    20341 tatcaaaaat cgaaaatttg gcggtaatgt caacgccgaa cgtatcgctt tactggcgat
    20401 gtaccacgat gcctcagaag tgccaccggc gatctcccta ctccggtgaa atacttcaat
    20461 tcgcaaatcg ctcaggagta caaggctatt gaaaaaatcg ctcagcaaaa actggtcgat
    20521 atggttccgg aagagctgcg ggatatcttt gcgccgttaa ttgacgagca tgcatatagc
    20581 gatgaagaaa aatcgctggt gaaacaggca gatgcactgt gtgcatatct gaaatgtctg
    20641 gaagaactcg cggccggaaa taatgaattc ttgctggcaa aaacgcgact ggaagcgacg
    20701 cttgaagcgc gtcgcagcca ggagatggac tacttcatgg aagtatttgt tcccagcttc
    20761 catctttcgc tcgatgagat tagccaggat tcaccgctgt aagcagccgg agtctgcgtc
    20821 gcatcaggca ataagcgccg gatgcgacat caggctcttg tcaaaacgga aacagcatcg
    20881 ggatcatcac cacacaaacc gccatcacga taatggtgaa cggtaccccc aacttcacaa
    20941 agtcactgaa gctgtaattt cccggaccta aaaccagtgt gttaacaggt gaagaaaccg
    21001 gtgtcataaa ggcggcggat gctgccatcg ccacgaccat cgcgaatgga taaggcgaca
    21061 cccccatcgt tttggcagca gccagcgcaa tcggagccat caacaccgcc gtcgcggtat
    21121 tagagataaa tagcccaata accgccgaca agacaaacaa acaccccagc atcatatgtg
    21181 gcccgtaacc accgccaatg tccatcagcc ctttcaccgc cagcgcgaca cctcccgttt
    21241 tctgtaatgc cacagcaaac ggcatcatcc caacgatcaa aataatgctc ggccagtgaa
    21301 tggatttata ggcgctttca gcatctatac agcggaattt ccccatcagc aggcaggcga
    21361 tgatagcggc aacaggatta ggaatttcat ctgtcagcat taacgccacc attagcacca
    21421 gacagaaaat ggcatggggt gcctggctgt gcgcgggcga tgcttcactc acctcttccg
    21481 gtaagttcag cgctacgaag tcgcggccct gtttggccag cataccgatc agtttccagt
    21541 tacccacaac caggatgata tcgcccagca gcagaggctc atccgccagc gaaccttcca
    21601 gcgccacgcc attgcgcttt agccccacca cattcagtcc gtagcgggta cgaaaaccaa
    21661 tttcgcgcac cgatttacca atcagttctg actcaggaat taatgaaatc tctgccatgc
    21721 ccacatcaag ggcctggtca gaaaaatact cgccgcgcag taccatcggc tccagcaatt
    21781 gctcactaca aaattgccgg agatcgacat cagccgcaga catatcaata agcaaaacgt
    21841 cacgcgcgcg aaattcagaa accccattaa cgttcacgat aacgcgacga aaacgccgcc
    21901 agcgttcaac accgatgacg ttagcgccat aacgctcacg taatttgaga tcatccagcc
    21961 gttgaccaat cattggcgat ccggggcgaa tagccagacg tcgcgcacgc ccggtcagtc
    22021 gatattcacg gataagatcg cgaaaggttc gacgcgtcca gccttcgcgc tgcggggtct
    22081 gggtatcccc tttcagcatg aaacgcatca ctaacatata caaaataccc agcaccagca
    22141 cgaccaggcc aacaggtgtt acgctaaaga aactgaagcc gtgatagcct tcacgcagca
    22201 attcactgtt gactaccagg ttcggcggcg tcgccaccag cgtcatcatg ccgctaatca
    22261 gcccggcaaa acttaacggc atcatcagac gtgacggcga agtttgcata cgcatggcaa
    22321 cgcttaacac cacgggaata aagatagcga caacgccggt tgaactcata aacgcgccaa
    22381 gccccgcgac ggtcagcatc aacaaaacca acattttgat ttcactgttg cccgccactt
    22441 tgaccagcca tgttcccatt acggtggcaa caccggtacg gaccaaacca tcgccaataa
    22501 taaacaaggc ggcaatcagg acaacgttag gatcagaaaa gccggaaaat acttcgggga
    22561 ccgtcagcgt tccgcttaat gcaaacgcga caataacaaa caaagcgacc gcatccatac
    22621 gcactctgcc cgtcgcaaac aagacgatgg caacggccag taatgaaaga acccaaatca
    22681 attcaccgtt cacaacttat ccttgttaat tgagggggat gacttgattc tgccataaaa
    22741 aagccccgac gagacggggc taaatcat
//
LOCUS       DAZPEB010000048        22695 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZPEB010000048 DAZPEB010000000
VERSION     DAZPEB010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22695
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.049"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(157..3306)
                     /gene="acrB"
                     /locus_tag="ACHURJ_001638"
     CDS             complement(157..3306)
                     /gene="acrB"
                     /locus_tag="ACHURJ_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFR1280627.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(3329..4522)
                     /gene="acrA"
                     /locus_tag="ACHURJ_001639"
     CDS             complement(3329..4522)
                     /gene="acrA"
                     /locus_tag="ACHURJ_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFR1280628.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDNKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTAPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            4664..5311
                     /gene="acrR"
                     /locus_tag="ACHURJ_001640"
     CDS             4664..5311
                     /gene="acrR"
                     /locus_tag="ACHURJ_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFR1280629.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            5439..8801
                     /gene="mscK"
                     /locus_tag="ACHURJ_001641"
     CDS             5439..8801
                     /gene="mscK"
                     /locus_tag="ACHURJ_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFR1280630.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLELQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIVGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYIDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            8862..8960
                     /locus_tag="ACHURJ_001642"
                     /pseudo
     CDS             8862..8960
                     /locus_tag="ACHURJ_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9013..9174)
                     /gene="rsmS"
                     /locus_tag="ACHURJ_001643"
     CDS             complement(9013..9174)
                     /gene="rsmS"
                     /locus_tag="ACHURJ_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFR1280631.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(9188..9715)
                     /gene="priC"
                     /locus_tag="ACHURJ_001644"
     CDS             complement(9188..9715)
                     /gene="priC"
                     /locus_tag="ACHURJ_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFR1280632.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRTT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            9785..10162
                     /locus_tag="ACHURJ_001645"
     CDS             9785..10162
                     /locus_tag="ACHURJ_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFR1280633.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            10315..10866
                     /gene="apt"
                     /locus_tag="ACHURJ_001646"
     CDS             10315..10866
                     /gene="apt"
                     /locus_tag="ACHURJ_001646"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFR1280634.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            10995..12866
                     /gene="dnaX"
                     /locus_tag="ACHURJ_001647"
     CDS             10995..12866
                     /gene="dnaX"
                     /locus_tag="ACHURJ_001647"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFR1280635.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVPSALEKAP
                     AKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELAAKLAAEAIERDPWAARVS
                     QLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGAQQKLAEALSTLKGSTVEL
                     TIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQTLRRFFDAELDEESIRPI"
     misc_feature    12259..12323
                     /gene="dnaX"
                     /locus_tag="ACHURJ_001647"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            12919..13248
                     /locus_tag="ACHURJ_001648"
     CDS             12919..13248
                     /locus_tag="ACHURJ_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFR1280636.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            13248..13853
                     /gene="recR"
                     /locus_tag="ACHURJ_001649"
     CDS             13248..13853
                     /gene="recR"
                     /locus_tag="ACHURJ_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFR1280637.1"
                     /translation="MQTSPLLTQLMEALRCLSGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            13963..15837
                     /gene="htpG"
                     /locus_tag="ACHURJ_001650"
     CDS             13963..15837
                     /gene="htpG"
                     /locus_tag="ACHURJ_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFR1280638.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            16018..16662
                     /gene="adk"
                     /locus_tag="ACHURJ_001651"
     CDS             16018..16662
                     /gene="adk"
                     /locus_tag="ACHURJ_001651"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFR1280639.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            16794..17756
                     /gene="hemH"
                     /locus_tag="ACHURJ_001652"
     CDS             16794..17756
                     /gene="hemH"
                     /locus_tag="ACHURJ_001652"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFR1280640.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTCEL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEYIEMMANLVAAYR"
     gene            complement(17753..18712)
                     /gene="aes"
                     /locus_tag="ACHURJ_001653"
     CDS             complement(17753..18712)
                     /gene="aes"
                     /locus_tag="ACHURJ_001653"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFR1280641.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYPLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIVGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            18864..20168
                     /gene="gsk"
                     /locus_tag="ACHURJ_001654"
     CDS             18864..20168
                     /gene="gsk"
                     /locus_tag="ACHURJ_001654"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFR1280642.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(20301..21977)
                     /gene="ybaL"
                     /locus_tag="ACHURJ_001655"
     CDS             complement(20301..21977)
                     /gene="ybaL"
                     /locus_tag="ACHURJ_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFR1280643.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(22215..>22695)
                     /gene="fsr"
                     /locus_tag="ACHURJ_001656"
     CDS             complement(22215..>22695)
                     /gene="fsr"
                     /locus_tag="ACHURJ_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFR1280644.1"
                     /translation="SYYTFYLMQKFGLSIQNAQLHLFAFLFAVAAGTVIGGPVGDKIG
                     RKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFSAILVYAQELLPGRIG
                     MVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLLGMLTIFLPDNRHKD"
BASE COUNT         5572 a         6010 c         5917 g         5196 t
ORIGIN      
        1 taaattaact aacaatcagt attttttatg aattttatcc gtggttaata ctggttttcg
       61 tatgagatcc tgagttggtg gttcaattac tccttaatgt tcgtaggtta tgcataaaaa
      121 aggccgcttg cgcggcctta gtgattacac gttgtatcaa tgatgatcga cagtatggct
      181 gtgctcgata tcttcattct tgcggctaaa gcggcggcga accaccacaa agaataccgg
      241 aacgaagaag attgccagta ccgttgcggt caccatcccg cccattacac cggtacctac
      301 tgcgttctgc gcgccggaac cagcaccagt actgataacc agcggcataa cgccgaggat
      361 aaacgccagc gaggtcatca ggatcggacg taaacgcatc cgcactgcat caagcgtcgc
      421 ttcaatcaga cctttacctt ctttatccat caagtctttg gcgaattcga cgataagtat
      481 cgcgttcttc gccgacaacc caatggttgt gagcaggcct acttggaagt aaacgtcatt
      541 ggtcaggcca cggaaggtgg cagccagcaa cgcaccgata acccccagcg gaacgaccag
      601 cataacggag aacggaatcg accagctctc gtacagcgcc gctagacaca ggaatacgac
      661 aatcaacgaa atcgcgtaca gtgaaggtgc ctggttgccg gagaggcgtt cctgatagga
      721 catccccgtc cagtcatagc caacaccggt aggcagtttg ctcgccagtt gttccattag
      781 ctccattgct tcaccggtac ttttacccgg tgccgcctgg cctaagattt ccatggatgg
      841 caggccattg taacgttcca gacgcggcga accgtactcc caacgagaag aggagaatgc
      901 cgagaacggc accatctgac catcagcagc acgaacatac cagtcgccga tatcatccgg
      961 cagcatacgg tatttcgctt ctgacatgac gtaaactttc ttcacacgac cgcggtcgat
     1021 aaagtcgttc acatagctac cgccccatgc agcgcccaga gtggtgttaa tgtcgttgat
     1081 agaaacaccc agcgcctgcg ctttttcctg gtcgatatca atcttaaact gcggggtatc
     1141 ttccagaccg tttggacgta cgctggtcaa catatcagga tgctttgctg cttctgcaag
     1201 cagctggtta cgcgcctgag tcagtttttc gtgaccaagg ccagcctggt caatcagctc
     1261 aaagtcaaag ccggttgcag tacccagttc cacgattgcg ggtaggttaa aggcgaaaac
     1321 catcgcatct ttgatttgcg agaaagcgcg tgttgcacgc atggtaatcg cttcaacttt
     1381 gttttcttcg cccggacgat cggcccagtc cttcaaggaa acgaacgcaa taccggtatt
     1441 ctgaccacgt cccgcaaagc cgaagccgtt aacggcgaac accgactcaa cgttgttctt
     1501 ttctttggtc agatagtaat gcgttacctc attgagcact ttctgcgtac gttcctgcgt
     1561 tgcacctgct ggcagctgaa ccatggtcat aaatacgccc tggtcctcat ctggcaagaa
     1621 ggagcttggc agacgcacga acagataggc catgccgacc acgatgatca gatacagcac
     1681 caggtaacgc cccgtactgc gcagaatacc gcctacgctg tcggtgtagt ggtgcgtgct
     1741 cttctcgaac atgcggttaa accagccgaa gaagcctttt ttaccttccc cgtgatcgcc
     1801 tttggcaatc ggtttcagca tggtggcaca aagagccgga gtcaggatca acgctaccag
     1861 taccgacagc gccattgctg aaacaatggt aatagagaac tgacgataga tagcaccagt
     1921 agaaccgcca aagaaggcca tcggtacgaa taccgctgac agtaccatcg cgataccgac
     1981 cagagcgccc tgaatctgcc ccatcgactt acgggtagct tcttttggcg gcaaaccttc
     2041 ttccgccata acacgctcca cgttttctac cacaacgata gcgtcatcca ccaacaagcc
     2101 gatggcgagc accatcccga acattgttag cgtgtttatc gagaagccaa aggcggcaag
     2161 gacggcaaag gtcccgagca ataccaccgg tacggcaatg gtcggaatca acgtcgcgcg
     2221 gaagttctgc aggaacagat acataaccag gaacacgagg atgatcgctt cgaccagcgt
     2281 tttaaccact tcgtgaatag agattttcac gaacggcgtg gtgtcgtacg ggtaaacaat
     2341 tttcagaccc gacgggaaga acggttccat cttcgccagt tcagcacgga ttgccgcagc
     2401 ggtatccagc gcatttgcac cggtcgccag cttgatcccc agaccggaag ccggttggcc
     2461 gttaaactct gcgatgatgt cgtagttctc accacccagc tcaatcttcg ccacatcacg
     2521 cagcaggacg cgggaaccat cctgattcac tttcagcagg attttgccga actcttcagt
     2581 agaggtcaga cgcgtctgag caataataga ggcgttaagc tgttggcctt tcaccggcgg
     2641 cgtaccaccg agctgacccg ccgcaacctg ggcgttctgc gctttgatgg cggtaatgac
     2701 atcaaccggc gttagctgga atttgttcag ctcattcggg ttcatccaga tacgcatcgc
     2761 gtactgtgaa ccgaacaact gaacatcacc cacgcccgac gtacggctga tggcatcttt
     2821 catattcgcc gctacgtagt cagagatatc ctcctgcgtc atggtgccat cggtgttgat
     2881 aacgccgaca accatcagga agctgctgga tgatttctca acgctcaccc cttgctgctg
     2941 aacttcttgc ggcagcaacg gcatcgccag ctgcagtttg ttctgcacct gaacctgcgc
     3001 gatatccgca tcagtaccag actcaaaggt cagggtgatc tgcacggtcc ccgtggagtc
     3061 actgttagag gacatgtaca tcaggttatc gataccgttc atattctgtt cgataacctg
     3121 tgtcaccgtg tcctgcactg ttttcgcatc agcgccgggg taggaggcgg agatcgttac
     3181 tgccggcggt gcaatcgtag gatattgcgc caccggcagt ttgaggatcg ccagcccccc
     3241 tgccaacatg atgataatgg cgatcaccca cgcaaaaatc gggcgatcga taaagaaatt
     3301 aggcatgtct taacggctcc tgtttaagtt aagacttgga ctgttcaggc tgagcaccgc
     3361 ttgcggcttg ctggttatta tcagcggtaa cttcttgtgc ttttacctgg acaccaggac
     3421 gcactttctg cagcccactt attactacgc gatcgcctgc tttcagacct tctgtcacca
     3481 gccacttatc gccaatagcc tggcttgcaa cgatcggacg ggtttccact ttgtcatccg
     3541 cgccaactac cagtacggtg gcatcaccac gcggcgtacg ggttacgccc tgttgcggga
     3601 ctaaaatagc gtttggatta agcccttctt ccagacgtgc gcgcacgaac atacccggca
     3661 gcagagtgtg atccgggttc gggaagatag cgcgtagggt gatagaccca gtggtctgat
     3721 caacggtaac gtcagagaat tccagcgtac cgtcctgcgg gaacttaatg ccgtcactgg
     3781 tgatcagcga cactttggct ttgccgttct cttgtttcag cgtgccattc gccagttcct
     3841 gtttcaggcg caggaagtcg ttgctggact gggtcacatc aacgtagatc ggatcaagtt
     3901 gctgcacggt tgccagcgca gttgcctgac cgttctgtac caatgcgcct tccgtcacgt
     3961 tcgacttacc aatgcgaccg ctaatcggag cggtgacttt ggtgtaagcc agattgatcc
     4021 gcgcagtttc aacggcagct ttcgccgcag ttaccgcagc attcgcctgt tgcgcatcag
     4081 ccagagcctg atcgtactct tgcttactga tgtactgagt accgagcagt ttctgataac
     4141 gattcaccgt caattgcgcg atattggctg cagcctgggc tttcgccaga tcacctttcg
     4201 cactgtcgta tgtcgcctga taggtcgcag gatcaatctg atagagagag acaccagctt
     4261 cgatgtcgct accttctttg aaattacgct tcaggataat cccgctaact tgaggacgaa
     4321 cttctgcgat ccggtaggca ctggtgcgac ccggaagctc ggttgtgatc tgcagaggtt
     4381 cagttttgac tgttactacg ccaacggcgg gcatctgctg gccaccttgt tgggcctgtt
     4441 tgttgtcaca tcctgttagg gctaagctgc ctgagagcat cagaacgacc gccagaggcg
     4501 taaaccctct gtttttgttc atatgtaaac ctcgagtgtc cgatttcaaa ttggtcaatg
     4561 gtcaaaagtt aataaaccca ttgctgcgtt tatattatcg tcgtgctatg gtacatacat
     4621 tcacaaatgt atgtaaatct aacgcctgta aattcacgaa catatggcac gaaaaaccaa
     4681 acaagaagcg caagaaacgc gccaacacat cctcgatgtg gctctacgtc ttttctcaca
     4741 gcagggggta tcatccacct cgctgggtga gattgcaaaa gcagctggcg ttacgcgcgg
     4801 tgcaatctac tggcatttta aagacaagtc ggatttgttc agtgagatct gggaactgtc
     4861 agaatccaat attggtgaac tagagcttga gtatcaggca aaattccctg gcgatccact
     4921 ctcagtatta agagagatat taattcatgt tcttgaatcc acggtgacag aagaacgacg
     4981 tcgattattg atggagatta tattccacaa atgcgaattt gtcggagaaa tggctgttgt
     5041 gcaacaggca caacgtaatc tctgtctgga aagttatgac cgtatagaac aaacgttaaa
     5101 acattgtatt gaagcgaaaa tgttgcctgc ggatttaatg acgcgtcggg cagcaattat
     5161 tatgcgcggc tatatttccg gcctgatgga aaactggctc tttgccccgc aatcttttga
     5221 tcttaaaaaa gaagcccgcg attacgtcgc catcttactg gagatgtatc tcctgtgccc
     5281 cacgcttcgt aatcctgcca ctaacgaata accctgaatc tgactccagg atttttcctg
     5341 gacattttcg tcgttgctat tctggttcac tgcgtcgtga tattcttgcg gtttgacttt
     5401 ttcaggtcgt tcttcaggtt cagaaacctt cattcatcat gactatgttc cagtattaca
     5461 aacgatcacg gcattttgtt ttttcagcat ttattgcttt tgtttttgtc ttgttatgcc
     5521 agaacacggc gtttgcgcgg gcgtcatcga atggtgatct gccgacaaaa gcggacctgc
     5581 aggcgcaact tgactcacta aataaacaaa aagatctttc tgctcaggac aaactggagc
     5641 tgcaggatct gacagataca ttagccaccc tcgataaaat cgatcgcata aaagaagaga
     5701 cagttcagct acggcaaaaa gtcgctgaag cgccggaaaa aatgcgccag gcgaccgcgg
     5761 cgttaacagc acttagcgat gtcgataacg acgaagaaac gcgcaaaatt ctgagtacgc
     5821 tttcgttgcg ccagctggaa actcgcgttg cccaggcgct ggacgatttg caaaacgcac
     5881 aaaacgatct ggcgtcttat aacagccagc tggtttcgtt acagacgcag cccgaacgcg
     5941 tgcaaaatgc gatgtataac gcttcgcagc agctgcaaca aattcgcagt cgtctggatg
     6001 ggactgatgt cggcgagact gccttacgtc ccagccagaa agtgttaatg caggctcagc
     6061 aggcgttgct gaatgcggag attgaccagc agcgtaaaag cctggaaggg aacaccgtct
     6121 tgcaggatac cttgcaaaag caacgtgatt acgtgacggc gaacagcgcc cgtctggagc
     6181 accagttgca gctgttgcaa gaagcggtaa acagcaagcg cctgacttta actgaaaaaa
     6241 cggcgcagga agccgtctcc ccggatgaag ccgcgcgtat tcaggctaat ccgctggtga
     6301 aacaggaact ggaaattaac cagcagttaa gtcagcgtct gattaccgcg actgaaaacg
     6361 gtaatcagtt gatgcagcaa aacattaaag tcaaaaactg gctggagcgg gcgctgcaat
     6421 cggaacgcaa tattaaagag cagattgccg tcctgaaggg cagcctgctg ttgtctcgta
     6481 tcctttacca gcaacaacaa acgctgccct cggcggatga actggaaaac atgaccaacc
     6541 gcatcgcgga tttgcgtctc gaacagtttg aagttaacca gcagcgtgat gcactcttcc
     6601 agagcgatgc gttcgtcaac aaactggaag aaggtcacac caacgaagtc aacagcgaag
     6661 ttcacgatgc gttattgcaa gtggttgata tgcgtcgcga attgctggat caactcaaca
     6721 aacagttggg taaccagctg atgatggcca ttaacctgca aatcaaccag cagcagttaa
     6781 tgagcgtatc gaaaaacctg aaatccatcc tgactcagca aatcttttgg gtgaacagta
     6841 accgtccaat ggactgggac tggatcaaag cgttcccgca aagcctgaaa gatgaattta
     6901 agtcgatgaa aatcacggtg aactgggaaa aagcctggcc tgccgttttt atcgctttcc
     6961 tcgccggttt gccgctgctg ttgattgtcg ggctgatcca ctggcgtctg ggctggctga
     7021 aagcgtatca acaaaaactg gcttccgctg tgggttccct gcgtaacgat agccagctca
     7081 acacaccaaa agcgatcctt atcgacctga tccgcgcgct gccggtgtgc ctgattattc
     7141 tcgcggttgg cttgattctg ttgaccatgc agctcaacat cagcgaactg ctatggtcgt
     7201 tcagcaaaaa actggcgatt ttctggctgg tgtttggcct gtgctggaag gtactggaga
     7261 agaacggcgt tgccgtgcgt cacttcggca tgccggaaca gcagaccagc cactggcgtc
     7321 ggcaaattgt ccgcatcagt ctcgcattgc tgcctatcca tttctggtct gtggtggcag
     7381 aactttcccc gctgcatctg atggatgatg tgctggggca agcgatgatt ttcttcaacc
     7441 tgctgctgat tgccttcctg gtatggccaa tgtgccgcga aagctggcgt gataaagagt
     7501 cgcacaccat gcgactggtc accattaccg tgctgtcgat tatcccgatt gcattgatgg
     7561 tgctgactgc cacgggttat ttctacacca cgctgcgcct ctccgggcgt tggattgaaa
     7621 ccgtttatct ggtgatcatc tggaacttgc tctaccagac ggtattgcgc ggcttaagcg
     7681 tagcggcgcg gcgtatcgcc tggcgtcgtg cgctggcgcg tcggcagaat ctggtgaaag
     7741 agggcgcaga aggtgccgaa ccgccggaag aacccaccat tgcactggaa caggttaacc
     7801 agcagacgct gcgtattacc atgttgctga tgtttgcgct gttcggtgtc atgttctggg
     7861 caatttggtc cgatttgatc accgtgttca gctatatcga cagcattacg ctctggcatt
     7921 acaacggtac tgaagctggc gctgcggtgg tgaaaaacgt caccatgggc agtctgttgt
     7981 ttgcgattat cgcctcaatg gtggcctggg cgttgattcg caacctgcct ggtttgctgg
     8041 aagtgctggt gctctcgcga ctgaatatgc gccagggcgc gtcgtatgcc attactacca
     8101 tccttaacta catcattatt gccgttggtg cgatgacggt gttcggatcg ctgggcgtct
     8161 cttgggataa actccagtgg ctggcggcag cattatccgt aggtcttggt tttggtttac
     8221 aagaaatttt cggtaacttc gtctccggtt tgatcattct gttcgaacgt ccggtgcgta
     8281 ttggcgatac ggtaaccatt ggtagcttct cggggacggt aagtaagatc cgtattcgtg
     8341 cgacaacgat taccgatttc gatcgcaaag aagtgatcat cccgaacaaa gcgtttgtta
     8401 ccgagcgtct gatcaactgg tcgttgactg acactactac gcgtctggtg atccgtctcg
     8461 gcgtggccta tggctccgat ctggaaaaag tgcgtaaagt gttactgaag gcggcgactg
     8521 agcacccaag ggtgatgcac gaaccaatgc cggaagtctt ctttacggcg tttggtgcca
     8581 gcacgttgga tcatgagctg cgtctgtatg tgcgtgaact gcgtgaccgt agtcgtactg
     8641 tcgatgagct gaaccgtact atcgatcagc tgtgccgtga aaacgacatc aacattgcct
     8701 ttaaccagct tgaagtgcat ctgcacaacg agaagggcga tgaggtgacg gaagtaaaac
     8761 gcgactacaa aggcgatgac ccgacgccag cggtagggta ataaccaaag ggggcgacat
     8821 ttagttgccc cgagattact gacaaagtgc gcgttgttca tgccggatgc ggcgtgaccg
     8881 ccttatccgg cctacgaaac cgcaagaatt caatgtattg caggagcggt gtaggcctga
     8941 taagcgtagc gcatcaggca gttttgcgtt tgcccacaat cttaggggac atttagcggc
     9001 cccatttatt tctcactttt ccgcctcatc atcgcgcgtt aatttctttt cataatcgcg
     9061 ttttacaata tccagcgcgc gcagcacggt gcgggctggg atctgatttt cttccagcag
     9121 cacaatcaaa tcgacagcca gtttgacatc gtcaggggca ttttccagtg acatactctc
     9181 tccattgcta gcgggttaag cgcgctagcc tgttttcgat tttttccagc gcatggcggc
     9241 agcgtgccag gcgcgcttca taggcttcca cttcacgatg cagcgtttgc tgttccacga
     9301 gatcggtcac ccgcgccagg cgggctctgc gttcggcaac catctcacgt agccgccgct
     9361 caaaatcctg atgctgaata cgtttacgct gccagcgggc aattttcggt ggcgcactgt
     9421 cccactcgcg caatgaccag gcggaggctt cacgcgcgat ggcttccagt tgtgccgcca
     9481 gatgttccgc cagccaggcc acttgcggca gctgttgctg ctcaactgca tgacgaagcg
     9541 cagccagatt atcgccagcc tcatcgagac aagcctgcag tgttgtcgta cgggtttgaa
     9601 aaaggtgtct gtcaaaacgg gcacttagcg tggcaaattg cgctaccggg gcacaacgct
     9661 gacgcagcgt agcgagctgt ccttccagtt tttccagcag cagggcggtt ttcacgataa
     9721 gacctcaatg aaaatgataa ttgttatgct aaagtagcca ctctgtaagc tgacaacact
     9781 caatatgcaa cgaatcattt taatcatcat tggctggctg gcggtagtgc tgggtacgct
     9841 gggcgtggta ttaccggtat taccgacgac gccgtttatc ctgctggcgg cctggtgctt
     9901 tgcccgttct tccccgcgct ttcacgcctg gttgctgtac cgctcatggt ttggcagcta
     9961 tctacgtttc tggcagaaac atcatgcgat gccgcgcggc gtcaaaccgc gggcgatttt
    10021 gcttattttg ctcacgtttg ccatttctct gtggttcgtc cagatgccat gggtgcgcat
    10081 catgttgctg gtaattctcg cctgtttgct tttctatatg tggcgaattc cggtgattga
    10141 tgaaaagcaa gaaaagcact gaagcacaac aatcgcagtt gcaattattg cgtacagcca
    10201 gtacattctg gcgttttcga gcacaggcgc aggcggtcaa aggttaaaca actgttactt
    10261 ttgatacgtt taaaacgcgc cgtgagtacc accgtaacaa gcaggcatac acttatgacc
    10321 gcgactgcac agcagcttga gtatctcaaa aatagcatca aaagcattca ggactaccca
    10381 aaacccggca ttcttttccg cgatgtcacc agcttactgg aagacccgaa agcttacgct
    10441 ctcagcatcg acttgctggt tgagcgttac aaaaatgcgg gcattaccaa agttgtcggc
    10501 accgaagcgc gtggcttctt gtttggcgct ccggtagctc tgggtctggg cgttggcttt
    10561 gtaccggtcc gtaaaccggg caaactgccg cgtgaaacca tcagtgaaac ttacgacctg
    10621 gaatacggca ccgatcagct ggagatccac gttgatgcca tcaaaccggg cgacaaagtt
    10681 ctggtggtgg acgatctgct ggcaaccggc ggcactatcg aagcgaccgt taaactgatc
    10741 cgtcgtctgg gtggtgaagt ggctgacgct gcgttcatta tcaacctgtt cgatctcggc
    10801 ggcgaacagc gtctcgaaaa acagggcatt accagctaca gccttgtccc gttcccgggc
    10861 cattaattat cgccagtctt gtgctgccca cgctacggac agcacaagat gtgcattcag
    10921 cctcgccgtt ctgacggggc tgtgttagca ttaccccttc gtgaatccac cttccagcgt
    10981 ttcagagcct gccaatgagt tatcaggtct tagcccgaaa atggcgccca caaacctttg
    11041 ctgacgtcgt cggccaggaa catgtgctga ccgcactggc gaacggcttg tcgttagggc
    11101 gtattcatca tgcttatctt ttttccggta cccgtggcgt cggaaaaacc tctatcgccc
    11161 gactgctggc gaaggggcta aactgcgaaa ccggcattac cgcgacgccg tgcggcgtgt
    11221 gcgataactg tcgtgaaatc gagcaggggc gctttgtcga tctgattgaa atcgacgccg
    11281 cctcgcgcac caaagttgaa gatacccgcg acctgctgga taacgtccag tacgctccgg
    11341 cgcgtggtcg tttcaaagtt tatctgatcg acgaagtgca tatgctgtcg cgccacagct
    11401 ttaacgcact gttaaaaacc cttgaagagc cgccggagca cgttaagttt ctgctggcga
    11461 cgaccgatcc acagaaattg ccggtgacga ttttgtcgcg ctgtttgcaa tttcatctca
    11521 aggcgctgga tgtcgagcaa attcgccatc aacttgagca catcctcaac gaagaacata
    11581 tcgctcacga gccgcgggcg ttgcaattgc tggcgcgcgc cgctgaaggc agcctgcgag
    11641 atgccttaag tctgaccgac caggcgattg ccagcggtga cggccaggtt tcaacccagg
    11701 cagtcagtgc gatgctgggt acgcttgatg acgatcaggc gctgtcgctg gttgaagcga
    11761 tggtcgaggc caacggcgag cgcgtaatgg cgctgattaa tgaagccgct gcccgtggta
    11821 tcgagtggga agcgttgctg gtggaaatgc tcggcctgtt gcatcgtatt gcgatggtac
    11881 aactttcgcc tgctgcactt ggcaacgaca tggccgccat tgagctgcgg atgcgtgaac
    11941 tggcgcgcac cataccgccg acggatattc agctttacta tcagacgctg ttgattggtc
    12001 gcaaagaatt accgtatgcg ccggaccgcc gcatgggcgt tgagatgacg ctgctgcgcg
    12061 cgctggcgtt ccatccacgt atgcctctgc ctgagccaga agtgccgcgc cagtcctttg
    12121 cacccgtcgc gccaacggca gtaatgacgc caacccaggt gccgccgcaa ccgcaatcag
    12181 cgccgcagca ggcaccgact gtaccgctcc cggaaaccac cagccaggtg ctggcggcgc
    12241 gccagcagtt gcagcgcgtg cagggagcaa ccaaagcaaa aaagagtgaa ccggcagccg
    12301 ctacccgcgc gcgtccggtg ccatcggcgc tggaaaaagc gccagccaaa aaagaagcgt
    12361 atcgctggaa ggcgaccact ccggtgatgc agcaaaaaga agtggtcgcc acgccgaagg
    12421 cgctgaaaaa agcgctggaa catgaaaaaa cgccggaact ggcggcgaag ctagcggcag
    12481 aagccattga gcgcgacccg tgggcggcac gggtgagtca actttcgcta ccaaaactgg
    12541 tcgaacaggt ggccttaaat gcctggaaag aggagagcga caacgcagta tgtctgcatt
    12601 tgcgctcctc tcagcggcat ttgaacaacc gcggtgcaca gcaaaaactg gctgaagcgt
    12661 tgagcacgtt aaaaggttca acggttgaac tgactatcgt tgaagatgat aatcccgcgg
    12721 tgcgtacgcc gctggaatgg cgtcaggcga tatacgaaga aaaacttgcg caggcgcgcg
    12781 agtccattat tgcggataat aatattcaga ccctgcgtcg gttcttcgat gcggagctgg
    12841 atgaagaaag tatccgcccc atttgatcgt aagcacagct tacgttcgtc atccttaacg
    12901 tgattgagag agaaacctat gtttggtaaa ggcggtctgg gtaacctgat gaagcaagcc
    12961 cagcagatgc aagaaaaaat gcagaaaatg caggaagaga tcgcgcagct ggaagtcacc
    13021 ggcgaatctg gcgcaggtct ggtaaaagtg accatcaacg gtgcacacaa ctgccgtcgc
    13081 gtagagatcg acccgagcct gctggaagac gacaaagaga tgctggaaga cctggtggct
    13141 gcagcattca acgacgcagc acgtcgtatt gaagaaacgc agaaagaaaa aatggcctct
    13201 gtatcctccg gaatgcagct gccgcctggc tttaagatgc cgttctgatg caaaccagcc
    13261 cgctgttaac acagcttatg gaagcactgc gctgtctgtc gggcgttggc ccgaagtcgg
    13321 cgcagcgtat ggcgttcacg ctgcttcagc gcgatcgtag cggcgggatg cgtctggcgc
    13381 aggcgctcac ccgggcgatg tcggaaatcg gccactgcgc cgattgccgc actttcaccg
    13441 aacaggaagt ctgtaacatc tgttcgaatc cgcgtcgtca ggaaaacggt caaatctgtg
    13501 tggtggagag tccggcggac atctacgcca ttgagcagac ggggcagttt tctggtcgtt
    13561 attttgtgtt gatggggcat ctgtcaccgc tggacggcat cggtccggat gatatcgggc
    13621 ttgatcgtct ggaacagcgt ctggcagagg aaaaaatcac tgaagtgatc ctcgccacca
    13681 atcccacggt tgaaggtgaa gctaccgcta actacattgc cgagctttgc gcgcaatatg
    13741 acgtggaagc tagccgaatc gctcatggcg taccggttgg cggcgaactg gaaatggtcg
    13801 atggcaccac gctgtcacac tcccttgccg ggcgtcataa gattcgtttt taagcaaacg
    13861 agagcaggat cacctgctct cgcttgaaat tattctccct tgtccccatc tctccctcat
    13921 cctgttttta accttaaaat ggcattattg aggtagacct acatgaaagg acaagaaact
    13981 cgtggttttc agtcagaagt gaaacagctt ctgcacctga tgatccattc tctctattcc
    14041 aataaagaaa tcttcctgcg tgagcttatc tctaacgcct ccgatgcggc ggacaagctg
    14101 cgtttccgtg cgctatctaa cccggacctg tacgaaggtg atggcgaact gcgcgttcgt
    14161 gtctctttcg ataaagacaa gcgcacgctg accatctccg ataacggcgt ggggatgacc
    14221 cgcgacgaag tgattgacca tctggggact atcgctaaat ccggtaccaa atcattcctc
    14281 gaatccctgg gttctgacca ggcgaaagac agccagctga tcggtcagtt tggtgttggt
    14341 ttctactctg cgtttatcgt ggccgacaaa gtgactgtgc gtactcgcgc ggcaggcgaa
    14401 aaaccagaaa acggcgtctt ctgggaatcg gctggcgaag gtgaatatac cgttgccgac
    14461 atcaccaaag aagatcgtgg tactgaaatc accctgcatc tgcgtgaagg cgaagacgag
    14521 ttcctcgatg actggcgcgt gcgttccatc atcagcaaat actccgacca tatcgcgctg
    14581 ccggtagaga tcgaaaaacg cgaagagaaa gacggcgaaa ccgttatctc ctgggagaaa
    14641 atcaacaaag cgcaggcgct gtggactcgt aacaagtcgg aaatcaccga tgaagagtac
    14701 aaagagttct acaaacacat cgcccacgac tttaatgatc cgctgacctg gagccacaac
    14761 cgcgttgaag gtaagcagga gtacaccagc ctgctgtaca tcccgtccca ggctccgtgg
    14821 gatatgtgga accgtgatca taaacacggc ctgaaactgt acgtccagcg tgtgttcatc
    14881 atggacgacg cagaacagtt catgccgaac tatctgcgct tcgtgcgtgg tctgattgac
    14941 tccagcgatc tgccactgaa cgtttcccgt gaaatcctcc aggacagcac ggtaacgcgt
    15001 aacctgcgca atgcgctgac caagcgtgtg ctgcaaatgc tggaaaaact ggcgaaagac
    15061 gacgcggaaa aataccagac cttctggcaa cagtttggcc tggtactgaa agaaggtccg
    15121 gcggaagatt tcgctaacca ggaagcgatc gccaaactgc tgcgttttgc ttctacccat
    15181 accgattctt ctgcgcagac cgtatctctg gaagactacg tttcccgcat gaaagaaggg
    15241 caggagaaaa tctactacat caccgcagac agctatgcgg cagcgaagag cagcccgcac
    15301 ctggaactgc tgcgtaagaa aggcatcgaa gttctgctgc tttccgaccg catcgatgag
    15361 tggatgatga actatctgac tgagttcgac ggtaaaccgt tccagtcggt gtctaaagtt
    15421 gacgagtcgc tggaaaaact ggctgacgaa gttgatgaga gcgcgaaaga agcggagaaa
    15481 gcactgactc cgttcatcga ccgtgtgaaa gccttgctcg gcgagcgcgt gaaagatgtc
    15541 cgtctgactc accgtctgac cgatacgcca gcgatcgttt cgaccgacgc ggacgaaatg
    15601 agcactcaga tggcgaaact gttcgctgcg gcgggccaga aagtgccaga agtgaaatac
    15661 atcttcgaac tgaacccgga tcacgtactg gtgaaacgtg cggcagatac tgaagatgaa
    15721 gcgaagttca gcgagtgggt agaactgctg ctggatcagg cgctgctggc agaacgcggc
    15781 acgctggaag atccgaacct gtttattcgt cgtatgaacc agctgctggt ttcctgatgt
    15841 catgccggat gacctttgtg tcatccggca tttttctttt catcatctgc actttccgca
    15901 aattatctcg ccattaaccg tttcagcctc aggtgccttt cttgaggcaa tcgcctgttg
    15961 gtggtatcgt ttatcgcttt ttcaaaaaat tcgacacatt ttaaggggat tttcgcaatg
    16021 cgtatcattc tgcttggcgc tccgggcgcg gggaaaggga ctcaggctca gttcatcatg
    16081 gagaaatatg gtattccgca aatctccact ggcgatatgc tgcgtgctgc ggtcaaatct
    16141 ggctccgagc tgggtaaaca agcaaaagac attatggatg ctggcaaact ggtcaccgac
    16201 gaactggtga tcgcgctggt taaagagcgc attgctcagg aagactgccg taatggtttc
    16261 ctgttggacg gcttcccgcg taccattccg caggcagacg cgatgaaaga agcgggcatc
    16321 aatgttgatt acgttctgga attcgacgta ccggacgaac tgattgttga tcgtatcgta
    16381 ggccgccgcg ttcatgcgcc gtctggtcgt gtttatcacg ttaaattcaa tccgccgaaa
    16441 gtagaaggca aagacgacgt taccggtgaa gaactgacta cccgtaaaga cgatcaggaa
    16501 gaaaccgtac gtaaacgtct ggttgaatac catcagatga ctgcaccgct gatcggctac
    16561 tactccaaag aagcggaagc gggtaacacc aaatacgcga aagttgacgg caccaagccg
    16621 gttgctgaag ttcgcgctga tctggaaaaa atcctcggct aatcaagccg cagcagatct
    16681 gctttcgggc aggtctgctc atccacctca ggcaatacct ctcacagcaa ttagttcttc
    16741 ttcctcactt ttccgctaca attatcaaca agttgaatcg ataagaggcg gtaatgcgtc
    16801 agactaaaac cggtatcctg ctggcaaacc tgggtacgcc cgatgccccc acacctgaag
    16861 cggtaaaacg ctatctgaaa caatttttaa gcgacagacg cgtggttgat acctcacggt
    16921 tgttatggtg gccattgctg cgcggcgtta ttttgccgct gcgctcgccg cgtgtggcga
    16981 agctgtatgc ctctgtctgg atggaaggtg gctcgccgct gatggtttac agccgccagc
    17041 aacagcaggc gctggcacaa cgtttaccgg agacgcctgt agcgctggga atgagctacg
    17101 gctcgccatc actggaaagc gccgtcgatg aactcctggc agagcatgta gatcatattg
    17161 tggtgctgcc gctttatccg caattctcct gttctacggt cggtgcggta tgggatgaac
    17221 tggcacgcat tctggcgcgc aaacgtagca ttccggggat atcgtttatt cgtgattacg
    17281 ccgataacca cgattacatt aatgcactgg cgaacagcgt acgcgcttct tttgccaaac
    17341 atggcgaacc ggatctgctg ctgctctctt atcatggcat tccccagcgt tatgcagatg
    17401 aaggcgatga ttacccgcaa cgttgccgca caacgacttg cgaactggct tccgcactgg
    17461 ggatggcacc ggaaaaagtg atgatgacct ttcagtcgcg ctttggccgg gaaccctggc
    17521 tgatgcctta taccgacgaa acgctgaaaa tgctcggaga aaaaggcgta ggtcatatac
    17581 aggtgatgtg cccgggcttt gctgcggatt gtctggagac gctggaagag attgccgagc
    17641 aaaaccgtga ggtcttcctc ggtgccggcg ggaaaaaata tgaatatatt ccagcgctta
    17701 atgccacgcc agaatatatc gaaatgatgg ctaatcttgt tgccgcgtat cgctaaagct
    17761 gagcggtaaa gaactgagcg ccgtcgcgaa gagcctcgtc ggcggttttc atcattcgtg
    17821 aataatgcaa aaaggcatgc agcgtacctg ggtagagttt gaactcacag ggctgctgat
    17881 gcgccgctaa cgtctggtaa agcagacggc tgtcatccag cagcggatcg aactccgccc
    17941 cggcaataaa acagggcgga acttcgcgag tgagatcatt attaaacaga cagtaatacg
    18001 gtgactcgcg gtccgcgtcg ttgcttaaat acgcctcttc atacatctgc aaatcctgtt
    18061 gcgttaagcc atcccagaca ccgcccaaca gacgacgagt cacggaatcc cgtaatccgt
    18121 aaagcccata ccacagcaaa acacccacaa ttttaccgca atcgatctgt ttatcacgca
    18181 accacaacgc actggcgagc gccagcatgg cacctgcgga atcaccggca aagccaatgc
    18241 gggacatatt gatttgataa tcctccgcct gctggtggaa ataacaacaa gcagccacaa
    18301 tttcctctat cgcttgcgga aaacgcgctt caggtgaaag ggggtaatca ataccaatca
    18361 ccgtacattg gctgtagctt gccagcaggc gcatgatgcg atcgtgggta tcgagattgc
    18421 cgagaataaa accgcctcca tgcaaataaa atagcgtcgc cgggctatct ggctgcggac
    18481 aaaagagacg tgtttccacc tgcccatatt ttgttggaac catgtaagct ctggttgcca
    18541 tttctggagc gcccgcattc cagaatcggc gctcaagcgt gtaatactgt cgttgctcag
    18601 caatcgttcc cgttgcgggc caggacggta aatccggctg aagagtattc acaacggtct
    18661 tcatttcagc agaaataagg tccagaacag gtagtttgtt ttccggcttc atacaaaaac
    18721 tcctttcaaa ttacgtcatt gtaaggaacc actgccatga aaatgcgatc ccgcctgctg
    18781 atattgaaac tggctgcgtc tcgcgcgctc ccgtcagatt gtgttaacat tcgccgctca
    18841 gttaaccacc cgtaaaaaca accatgaaat ttcccggtaa acgtaaatcc aaacattact
    18901 tccctgtaaa tgcacgcgat ccgctgcttc agcaattcca gccagaaaac gaaaccagcg
    18961 ccgcctgggt cgtaggtatt gaccagacgc tggtcgatat tgaagcgaaa gtggatgatg
    19021 aattcattga gcgttatgga ttaagcgccg ggcattcact ggtgattgag gatgacgtag
    19081 ccgaagcgct ttatcaggag ctaaaacaga aaaacctgat tacccatcag tttgcgggtg
    19141 gcaccattgg taacaccatg cacaactact cggtgctcgc ggacgaccgt tcggtgctgc
    19201 tgggcgtcat gtgcagcaat attgaaattg gcagttatgc ctatcgttac ctgtgtaaca
    19261 cttccagccg taccgatctt aactatctac aaggcgtgga tggcccgatt ggtcgttgct
    19321 ttacgctgat tggcgagtcc ggggaacgta cctttgctat cagtccaggc cacatgaacc
    19381 agctgcgggc tgaaagcatt ccggaagatg tgattgccgg agcctcggca ctggttctca
    19441 cctcttatct ggtgcgttgc aaaccgggtg aacccatgcc ggaagcaacc atgaaagcca
    19501 ttgagtacgc gaagaaatat aacgtaccgg tggtgctgac gctgggcacc aagtttgtca
    19561 ttgccgagaa tccgcagtgg tggcagcaat tcctcaaaga tcacgtctct atccttgcga
    19621 tgaacgaaga tgaagccgaa gcgttgaccg gagaaagcga tccgttgttg gcatctgaca
    19681 aggcgctaga ctgggtagat ctggtgctgt gcaccgccgg gccaatcggc ttgtatatgg
    19741 cgggctttac cgaagacgaa gcgaaacgta aaacccagca tccgctgctg ccgggcgcta
    19801 tagcggaatt caaccagtat gagtttagcc gcgccatgcg ccacaaggat tgccagaatc
    19861 cgctgcgtgt atattcgcac attgcgccgt acatgggcgg gccggaaaaa atcatgaaca
    19921 ctaatggagc gggggatggc gcattggcag cgttgctgca tgacattacc gccaacagct
    19981 accatcgtag caacgtacca aactccagca aacataaatt cacctggtta acttattcat
    20041 cgttagcgca ggtgtgtaaa tatgctaacc gtgtgagcta tcaggtactg aaccagcatt
    20101 cacctcgttt aacgcgcggc ttgccggagc gtgaagacag cctggaagag tcttactggg
    20161 atcgttaagt tatcgtcggt tcgtaggcca gataaggcgt tcacgccgca tctggcattt
    20221 ggctctcgat gcctgatgcg acgctggcgc gtcttatcat gcctacatat ttttcatatt
    20281 ttacatccgg caaccaccgt ttaccccgtc accacctcac ccgccggtgg cgtttccagc
    20341 agttccagca tggtacgggc gatttcacgc tcgcccatca ctacctgatt cgcaccacgt
    20401 tcggtgatat acgccacttc atcgtcataa tgggcgcggg caataatctc aatatccgga
    20461 tttttcgcgc gggcagatgc cacaatctca cccgcttcat aaccgttggg aatcgtcagg
    20521 atcagccatt ttgcacattc cagatgcgcc agttgcataa tttcttcgtt cgccgcattg
    20581 cccaatactg cgcggacccc gcgctctcgc agctcatcaa cacgggttcg tgacgtctca
    20641 atcaccacca gcggaatatc agaggcgagc aatttctccc ccagcaggct gcctacacga
    20701 ccgtaaccca ccagtagcgc atggttgcaa atatccactg ggatctgctt ctcttcttcg
    20761 attgcctctt ccagcgtctg ctcttccagc gtttcggtct tcgccagata tttctccagt
    20821 agtgcgaaca gtaccgggtt gagcataatc gacaggatcg cccctgccag taccaggttt
    20881 tgtccggcct gcggcagtaa attcaatgcc attcccagtc ccgccaggat aaacgcgaac
    20941 tcaccaatct gcgccaggct ggcggcgatg gttaatgccg tacgttggga gtgaccaaac
    21001 agtcgcacca ggaaaaatgc ggctaacgac ttaccaaaca gaataatcgc cagcgtcgcc
    21061 agcactgcca gcggttgctg aatcagaatt aacggatcaa acaacatccc gacggagaca
    21121 aaaaacagca ccgcaaacgc gtcgcgcaat ggcagcgtat cgtgggcggc acggtgactc
    21181 agttcagact cgttcagtac catcccggca aagaacgcac cgagtgcaaa ggagacatca
    21241 aacagctcta ccgcaccaaa ggcaatccct aacgccagcg ccagcaccga cagggtaaac
    21301 agctcgcgag aaccggttgc cgcgctgcgt gccataatcc acggcaccag acggcgacct
    21361 accagcatca taatggcgat aaatgcgatc actttgccga tggtgatccc catatcgact
    21421 gcaagagtgg caaagcccac atcgccctgt tccatcattc ctgccactgc gggcagcaac
    21481 accagcgtca gaaccattac caggtcttcc acaatcaacc aaccgatggc gatttgccca
    21541 cgctgactgt caattaattg ccgttcttca agtgcgcgca gtaacaccac ggtactggcg
    21601 gtggaaagac ataaaccgaa cacgataccg gtcattaacg accagcccag cacggcagag
    21661 agcgccatac ccagcagcgt cgccacggct atctgggcga tcgcaccggg aatggcgatg
    21721 gcctttaccg ccatcaaatc cttcagcgaa aagtgcaaac cgacgccaaa catcaacaga
    21781 atgacgccca gttcagccag ttccggcgca agcttggtat cggcaacaaa gcccggagtg
    21841 aatggtcctg ccagcacacc cgctaacaga tatcccacca gaggagaaat acgtagttta
    21901 ttggccagca tgccgaggat aaaggcgagc acaaggccgc caacaatggt ggtgataagc
    21961 ggggtggcgt gatgcattcc gtctcctttt cctggtggtt attgtccatt tttggccggg
    22021 aaaaccaaaa ttacaggtaa tagtttatga caatttcatt gatgatgttc atgaataatt
    22081 attgaatttt gcagaaaaat ggaattagct gcaaaaaaag cacggatcgg aaaacagaag
    22141 gcggtttaac agaggaaagg cttatggcga ctgtaaaacg atgcagccaa agtttgcctt
    22201 tggctgcaat gaaatcagtc tttatgccgg ttatcaggca ggaatatggt caacatcccc
    22261 aatagtggca ggaaagcaca gattttatag actaactcga tgctggtgtg atcggcgata
    22321 agccccagaa ctgccgctcc cagacctccc atgccaaaag caaaaccgaa aaagagtcca
    22381 gaaaccatac cgatacgtcc cggaagtagc tcctgagcgt agaccagaat ggcagagaat
    22441 gccgaagcga ggataaatcc aataatcacc gttaaaaccc ccgtccagtg caggctggcg
    22501 tagggtaaaa tcagcgtaaa cggcgcaacg ccgaggatag agccccaaat cacatatttc
    22561 cgtccaattt tatcccctac aggcccgccg atcaccgtac ctgccgcaac ggcaaacagg
    22621 aaggcaaaca gatgaagctg ggcattctgg atagataatc cgaatttttg catcagataa
    22681 aaggtgtaat agctg
//
LOCUS       DAZPEB010000049        22588 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZPEB010000049 DAZPEB010000000
VERSION     DAZPEB010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22588
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.050"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..257)
                     /locus_tag="ACHURJ_001657"
     CDS             complement(<1..257)
                     /locus_tag="ACHURJ_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFR1280645.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGICTGEGLEIVGQGDC"
     gene            complement(248..718)
                     /locus_tag="ACHURJ_001658"
     CDS             complement(248..718)
                     /locus_tag="ACHURJ_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFR1280646.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(903..1697)
                     /gene="thyA"
                     /locus_tag="ACHURJ_001659"
     CDS             complement(903..1697)
                     /gene="thyA"
                     /locus_tag="ACHURJ_001659"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFR1280647.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYAPHPGIKAPVAI"
     gene            complement(1704..2579)
                     /gene="lgt"
                     /locus_tag="ACHURJ_001660"
     CDS             complement(1704..2579)
                     /gene="lgt"
                     /locus_tag="ACHURJ_001660"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFR1280648.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(2730..4976)
                     /gene="ptsP"
                     /locus_tag="ACHURJ_001661"
     CDS             complement(2730..4976)
                     /gene="ptsP"
                     /locus_tag="ACHURJ_001661"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFR1280649.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGLPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPILLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEDEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(4989..5519)
                     /gene="rppH"
                     /locus_tag="ACHURJ_001662"
     CDS             complement(4989..5519)
                     /gene="rppH"
                     /locus_tag="ACHURJ_001662"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFR1280650.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNTSAYRRKRG"
     gene            complement(5872..6018)
                     /gene="ygdT"
                     /locus_tag="ACHURJ_001663"
     CDS             complement(5872..6018)
                     /gene="ygdT"
                     /locus_tag="ACHURJ_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFR1280651.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            6204..6893
                     /gene="mutH"
                     /locus_tag="ACHURJ_001664"
     CDS             6204..6893
                     /gene="mutH"
                     /locus_tag="ACHURJ_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFR1280652.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWTPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            6962..7675
                     /locus_tag="ACHURJ_001665"
     CDS             6962..7675
                     /locus_tag="ACHURJ_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFR1280653.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            7813..8031
                     /gene="ygdR"
                     /locus_tag="ACHURJ_001666"
     CDS             7813..8031
                     /gene="ygdR"
                     /locus_tag="ACHURJ_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFR1280654.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            8139..9179
                     /locus_tag="ACHURJ_001667"
     CDS             8139..9179
                     /locus_tag="ACHURJ_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFR1280655.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPSIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKTAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(9211..10404)
                     /gene="lplT"
                     /locus_tag="ACHURJ_001668"
     CDS             complement(9211..10404)
                     /gene="lplT"
                     /locus_tag="ACHURJ_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFR1280656.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNSTPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(10397..12556)
                     /gene="aas"
                     /locus_tag="ACHURJ_001669"
     CDS             complement(10397..12556)
                     /gene="aas"
                     /locus_tag="ACHURJ_001669"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFR1280657.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDASRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTLDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            12945..13976
                     /gene="galR"
                     /locus_tag="ACHURJ_001670"
     CDS             12945..13976
                     /gene="galR"
                     /locus_tag="ACHURJ_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFR1280658.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(13983..15245)
                     /gene="lysA"
                     /locus_tag="ACHURJ_001671"
     CDS             complement(13983..15245)
                     /gene="lysA"
                     /locus_tag="ACHURJ_001671"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFR1280659.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSVPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRSLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            15367..16302
                     /gene="lysR"
                     /locus_tag="ACHURJ_001672"
     CDS             15367..16302
                     /gene="lysR"
                     /locus_tag="ACHURJ_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFR1280660.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     MIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASICAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(16289..16981)
                     /gene="ygeA"
                     /locus_tag="ACHURJ_001673"
     CDS             complement(16289..16981)
                     /gene="ygeA"
                     /locus_tag="ACHURJ_001673"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFR1280661.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(17110..18525)
                     /gene="araE"
                     /locus_tag="ACHURJ_001674"
                     /pseudo
     CDS             complement(17110..18525)
                     /gene="araE"
                     /locus_tag="ACHURJ_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
     gene            complement(18840..19601)
                     /gene="kduD"
                     /locus_tag="ACHURJ_001675"
     CDS             complement(18840..19601)
                     /gene="kduD"
                     /locus_tag="ACHURJ_001675"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFR1280662.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(19631..20467)
                     /gene="kduI"
                     /locus_tag="ACHURJ_001676"
     CDS             complement(19631..20467)
                     /gene="kduI"
                     /locus_tag="ACHURJ_001676"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFR1280663.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLHNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(20754..21935)
                     /locus_tag="ACHURJ_001677"
     CDS             complement(20754..21935)
                     /locus_tag="ACHURJ_001677"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFR1280664.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PCTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            22190..>22588
                     /locus_tag="ACHURJ_001678"
     CDS             22190..>22588
                     /locus_tag="ACHURJ_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HFR1280665.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLG"
BASE COUNT         5794 a         6048 c         5440 g         5306 t
ORIGIN      
        1 atacagtcac cctgtccgac aatttccagc ccttcgccgg tacagatgcc atgacaataa
       61 cccgccctct ggagatgctt cgcgacggta aatacccgca gccagatttc atcttccagc
      121 gccagcttac gggtgctcgt taaactttca cgctgtaacg caggcagaaa gcgtgcagcc
      181 cccagcaaca atacgctact gatcgccata gcaatcaaca cttccagcag agaaaaacct
      241 tgctctttta caggcatcct tctgtttctc cttgctgaca aagccggagt cttccccacg
      301 gcgaaaccac cagccaccac tcgcccgttg agtttttgaa gcgaatatgc ccggcccatg
      361 cggtattgcg caggccaaag aaagcaagcg aaggtgtcag gtcgctcatt tcgacttcgg
      421 gccagcgtgg cacaaagacc aatggtgaac tgccatgaca ggtattggcc ccagcagcgg
      481 aactcacaag gcaccataac gtcccctccc tgataacgct gatactgtgg tcgcggttat
      541 gccagttggc atcttcacgt aaatagagca aatagtcccg cgcctggctg gcggtttgcc
      601 atagccgttg cgactgctgc cagtattgcc agccatagag tccacttgcg cttagcatga
      661 ccaaaatcag catcgcgacc agcgtttcaa tcagcgtata accacgttgt gttttcatgc
      721 cggcagtatg gagcgaggag aaaaaaagac gagggccagt ttctatttct tcggcgcatc
      781 ttccggacta tttacgccgt tgcaggacgt tgcaaaattt cgggaaggtg tctcgaagaa
      841 tttaatggag ggtaaaaaaa accgacgcac actagcgtcg gctctggcag gatgtttcgt
      901 aattagatag ccaccggcgc tttaatgccc ggatgcggag cgtagccttc aatctcaaaa
      961 tcttcgaaac ggtagtcgaa gatggattcg ggtttacgtt tgataatcaa cttcggcagc
     1021 ggacgcggtt cgcggcttaa ttgcagatga gtttgatcca tatgattgct gtacaggtgc
     1081 gtgtcgcccc ccgtccagac aaaatcgccc acttccagat cacactgctg cgccatcata
     1141 tgcaccagta acgcgtagct ggcaatgtta aacggcaggc cgaggaaaac gtcacaggag
     1201 cgctgataaa gctggcaaga gagtttgccg tctgccacat agaactggaa gaacgcatgg
     1261 cacggtgcca gcgccatttt atccagttcg cctacgttcc acgctgaaac aataatgcgg
     1321 cgcgaatccg ggtcgttttt cagctggttc agtaccgtag tgatctggtc aatatgacga
     1381 ccatccggcg ttggccaggc gcgccactgt ttaccataca ctggcccgag gtcgccgttt
     1441 tcatcggccc attcgtccca gatggtgaca ttgttttcgt gtagataagc aatgttagtg
     1501 tcgccctgca gaaaccacag cagttcatgg atgatggaac gcaggtggca acgtttagtt
     1561 gtcaccagcg ggaatccatc ctgcaggtta aaacgcatct gatgaccaaa aatggaaagc
     1621 gttccggttc cggtacggtc gtttttctgt gtgccttcgt cgagcacttt ttgcatcagt
     1681 tctaaatact gtttcatggt tcctcaggaa acgtgttgct gtgggctgcg acgatatgcc
     1741 cagaccatca tgatcacacc cgcgacaatc atcgggatgg aaagaatttg tcccatgctg
     1801 atgtactgca cccaggcacc agtaaactgc gcgtcgggct ggcggaaaaa ctcaacaatg
     1861 atgcgaaacg cgccgtaacc aatcaggaac aaacctgaga cagctcccat tgggcgcggt
     1921 ttacgaatat acaggttgag gataataaac agcactacac cttccagcaa cagctcgtaa
     1981 agctgtgatg ggtggcgcgg cagcacaccg taagtatcga aaatggattg ccactgcggg
     2041 ttggtttgca gcagcaaaat atcttctgta cgggagccag ggaacagcat ggcaaacggg
     2101 aagttcgggt caacgcggcc ccacaattca ccgttaataa agttgcccag acgcccggca
     2161 ccaagaccaa acggaatgag tggtgcgata aaatcagaga cctggaagaa ggaacgttta
     2221 gtacggcggg cgaagataat catcaccacg ataacgccaa tcaggccgcc gtggaaggac
     2281 atgccgccgt cccagacacg gaacagatac agcggatcgg ccataaactg cgggaagttg
     2341 tagaacagaa cataaccaat acgtccaccg aggaagacgc cgaggaagcc cgcatagagt
     2401 aagttttcaa cttcattttt ggtccagcca ctgcccggac gattcgcccg acgtgttgcc
     2461 agccacattg caaaaatgaa acccaccaga tacatcaggc cgtaccagtg aagcgccacg
     2521 ggtcctattg agaaaatgac cggatcaaac tccggaaaat gcagatagct actggtcatc
     2581 tgtcaccaca agttcttgtt atttcgctga aagagaacag cgattgaaat gcgcgccgca
     2641 ggtttcaggc gctccaaagg tgcgaataat agcacaaggg gacctggctg gttgccggat
     2701 accgttaaaa gatatgtata tgatccgcgc tataaccctc cgcgaatcag cccgcccatg
     2761 ccgcgacgct ccataaaggc tgcaacctga tgacgaactt cggtcgccag ttgcgcttcc
     2821 agactacgct gcgcaagatt ttctgcttcg gcaaaatcaa tgcgccgcag caggtatttt
     2881 acccgcgcta cagaacgtcc gttcatagac agatggcgat acccaagccc aatgaggatt
     2941 gccacgcaca tgggatcgcc cgccatttca ccgcacaaac ggagatcgat accatgtatt
     3001 tccgcttccc gggcgatcat tgccagagct cgtaacattg caggatgaag gctgtcataa
     3061 atgttcgcca cccgggtatt gttgcgatca acggccagaa tgtattgagt cagatcgttg
     3121 gtgccaacag agatgaaatc gacccgcttt gccagatgcg gcagcataaa taccattgac
     3181 ggtacttcca gcatgatgcc gatacgtggt ttgggaattt cgtaaccgat catctcctcg
     3241 acttcacgtc cggcacgttc aatcaggcgg cgtgcttcgt caacttcatc gaggctggtg
     3301 accatcggca acagaatatt caggttgccc gtagcggcat tagcacgcag catcgcccgc
     3361 acctggatca agaagatctc cggctgatcg agcgtaatgc gaatcccacg ccaacccagg
     3421 catggattct cttcgctgat cggcatgtaa ggcagctgct tatctgctcc gacatccagc
     3481 gtacgcaagg tgacgggttt atcattgaac atttgcagca tcccctgata ctgcgccacc
     3541 tgttcatctt ccgacgggaa accactttgc agcatgaatg ggatttcagt gcgataaaga
     3601 ccgatgccat caatacggct gcccagtttt tcttcatgtt ccgggcttaa accagcattg
     3661 agcatgactt ttatacgctc accgcttttt aactgcgcgg gtaaattgac gtcatcttcc
     3721 gccagacggc taagctcaat ctcttcacta attagccgct gatattcttg cagcagtatc
     3781 ggctccggat cgaccagcaa ttcaccgcga tagccatcaa cgatcagcgt cctacgatgc
     3841 agcaccgaag gctgaatatc cgcgcccatc acggtaggga gccccagcgc acgtaccatg
     3901 atcgcagcat gggagttggc tgcgccatct cgcacgacaa caccgactaa gcgatcctgg
     3961 ggcagctcag caagagttgt cgctgacagt tcatctgcca ccagaatgaa acgttccggc
     4021 caggcgttcg gcccttgatt agcgtcatca agatgaaaca gcaatcgctg acccagcgca
     4081 cgtaaatcgc cagcccgctc tttgagatag ttatcgctta gcgcggcaaa ctgttcggca
     4141 aatttttcaa tgaccgtttt taccgcccac tctgccaccg agcctttatc aacctcggca
     4201 aacaattcgc gacgcagccg ggtgtccgaa agcaggtgcg agtaaagatc gaaaatagcc
     4261 gccgtttctt tttgtgctcc ggcggcaaag cgtttgctgt agcggcgaaa ctcgtttgcc
     4321 gcttcttcca gcgccccggt caatcgttcg cgttccagag ccggatccag cgttgatgcc
     4381 tgatacacct gttccattaa aggtaacgtg gcatcctgcc agccttcggc aatcgccaca
     4441 ccaggtgctg ccggtaatgc gcggattcgc gtctggcgat attgcccaaa caaggcagtc
     4501 aactgcgact gagaaagaat agctgccatc tgggtggcaa gcgtcaccag gaaggattct
     4561 tcactttcgt catactggcg caactctcgt tgctgtacca ccagtacacc aagcaactgg
     4621 cgacgttgaa taattggtac gcctaaaaac gcgcggaaac gttcttcttt tacggagggg
     4681 atgtatttga agctggggtg cttttgcgca tctgcaaggt ttatcggttc cgccagcctg
     4741 ccaaccaggc cgacgatccc ttcatcaaac gcgagcgtta cagtgcgacc gcgtggtttt
     4801 ttcagccccc gggtcgccat caggtagtaa caacgtcgat catgatcggc caggtagacc
     4861 gaacagacct cggtatccat cgcaagacag atgtcggtaa ccagaatatt taacgcctca
     4921 ttcaggcgtg gtgcgctggc taccttttcg actatttcgc gcaggcgagt gagcataatt
     4981 ggcgtgactt aacctctttt acgtcgataa gcagatgtgt tttgtggttt tggcgtattt
     5041 tcctgcagtg acatcaccac actcgcgaac tctttcatta ccctacggta gacatcacgt
     5101 ttaaatgaca ccacctgtct gaccggatac cagtaactta cccatcgcca gccgtcaaac
     5161 tctggtgtac tgctggtttg catattgatt tctgcatcgc cgctcaccag ctgcaagaga
     5221 aaccattttt gtttttggcc gatacaaacc ggcttcgtgt cccaacgcac caaacgtttc
     5281 ggtaatttgt agcgcaacca gttacgcgtt gaagcaagga ttcgaacgtc tttgcggctt
     5341 aatcctactt cttcaaacaa ttcacggtac atcgcctgct ctgcggattc tccggggttg
     5401 attccgcctt gcggaaattg ccaggagtgc tgaccaaatc gccgggccca cattacctgc
     5461 ccctggcgat tacaaatcac gatacctacg tttgggcggt agccatcgtc atcaatcacc
     5521 ggactacctc aaaataaagc tttatatacg aatgattgtt tcatactcca ggaagacggt
     5581 aaaccactct ctgcagggca ttacacacta ataacaattg aataactcac agttatgtgc
     5641 agagttataa acagaggaag gggtggatag ccgtttttat tcaccttttc tgtggataga
     5701 gttgtgaaga actacggaat tactacggga aaacccggag cattctgaat aagcccgata
     5761 taggcatttt aaaatattca ttataagtca atgcattaca tgtaatttga gtaatgaaaa
     5821 agagtaattc gtgacccagg tcacacctct catttacggg ttggcgaaag atcaaccaat
     5881 gccgtattta tccacagaat gtgccactaa gttaagcact gaaccactaa aaactggagt
     5941 ttcgtcgcac gtcaaggctg taaatggaaa cagtagtgga ggtttttcac agttatccca
     6001 gctttctgtg gataacatgg tgtaagatcc tgtttatttt cagtgaccag atttggaaaa
     6061 cctattgcag tgctgcgcaa ctcgattatc ggcagcctaa aaagcaatat aaatcagaga
     6121 attgaacaac acatgtggaa aaagttacac tgcgaatatt cggtacataa ttgctgtttg
     6181 ttttttaacc aaggtatcat gacatgtccc aacctcgccc actgctctct cctcccgaaa
     6241 ctgaagaaca gttgttagcg caagcacagc aactttctgg ttatacattg ggagaactgg
     6301 cggcacttgc cgggctggtt acgccagaga atttaaaacg cgataagggc tggattggcg
     6361 tgttactgga gatctggcta ggtgccagcg cagggagtaa acctgagcaa gattttgctg
     6421 ctctgggcgt ggaacttaaa actatccctg tggatagtct tggtcgtccg ctggaaacaa
     6481 cattcgtttg tgttgccccg ttaacgggta atagcggggt gacctgggaa accagccacg
     6541 tgcgccacaa gctcaaacgc gtactgtgga taccggttga aggcgagcgc agcatcccgc
     6601 tggcgcagcg tcgcgtagga tccccgttgc tgtggacccc gaatgaagag gaagaccgac
     6661 agctacgcga agactgggaa gaattaatgg atatgattgt tctcggtcag gttgagcgga
     6721 tcaccgctcg tcacggggaa tatttacaga taagaccgaa agcagcgaat gcgaaagcgc
     6781 ttaccgaagc cattggtgcc cggggcgaac ggattctgac gctgccacgc ggcttttatt
     6841 tgaagaagaa tttcaccagt gcactactgg cccgtcattt tctgatccag tagccatcgc
     6901 tttgacctgc cgctttccgg gcatataatt accgcttcat ttttttggca gggcttttta
     6961 gatgttattt gcatggataa ccgatcctaa cgcctggctt gcgctcggta cgctgacgct
     7021 gctggagatc gttcttggga tcgacaatat tattttcctt tctctggtgg tggcaaagct
     7081 tcccacagca caacgtgctc atgcgcgccg tctggggttg gcgggagcca tggttatgcg
     7141 tctggcgctg ctggcatcaa tcgcctgggt tacgcgcctg acaaatccgc tttttacaat
     7201 attcagtcag gaaatttctg cccgtgattt gattctgctt ctgggtggct tgttccttat
     7261 ctggaaagcc agcaaggaaa tccacgaatc cattgaaggt gaagaagaag ggctgaaaac
     7321 acgcgtttca tcattcctcg gcgctatcgt gcagattatg ctgctggata ttatctttag
     7381 cctcgactcg gtgattacgg ctgtgggtct gtcagatcac ctgtttatta tgatggcagc
     7441 cgtggtgatt gccgtaggcg tgatgatgtt cgccgcgcgc tcgattggtg attttgtcga
     7501 acgccatcct tcggtaaaaa tgctggcgct ctctttcctg attctggtgg gctttaccct
     7561 gattctggaa agtttcgaca tccacgtacc gaaaggttac atctacttcg cgatgttctt
     7621 ctctattgcg gttgaaagcc tcaacttgat tcgcaacaaa aagaatccgc tctgatactc
     7681 cgtacgctct cctgcgggag ggcgtactca ctccttttac cctcacagtt taagattttc
     7741 ctgctttcaa gatatataac gtcgttttat aaacagacta ttatcatagg taggccaaat
     7801 gaggataacc ggatgaaaaa atgggcagta ataatttctg cagtcggact ggcgtttgct
     7861 gtttccgggt gttccagtga ttacgtcatg gcgaccaaag atggccgtat gattttgacc
     7921 gatggaaaac ctgaaattga tgatgatacc gggctggtga gttatcacga tcagcaaggt
     7981 aacgcgatgc aaattaaccg tgatgatgtt tcgcaaatta ttgaacgtta acaaataagg
     8041 tcagcatccg gctggcctta agatttttct ctttcccttt tcccttccct ctgccatttt
     8101 tatattcctt atgtcgtgat tataaaaagg aaacggctat gcaatatcac cgtatacccc
     8161 acagttcgct ggaagtcagc acgctggggc ttggcacgat gacgtttggt gaacagaaca
     8221 gcgaagccga cgcccacgca caactcgact atgccgtcgc tcagggcatt aaccttatcg
     8281 acgttgccga aatgtaccca gtacctccgc gccccgaaac gcaagggtta accgaaacct
     8341 acgtcggcaa ctggctggcg aaacatggca gccgcgaaaa gttaattatc gcctccaaag
     8401 tgagcggacc gtcgcgcaat aatgacaagg gcatccgccc ggatcaggcg ctggatcgga
     8461 agaatatccg cgaagcgctg catgacagcc tcaagcgcct acagactgat tacctcgatc
     8521 tttatcaggt gcactggccg cagcgcccga ccaactgctt cggcaaactc ggttatagct
     8581 ggacggattc tgcgcctgcg gtttcgctgc tggatacgct ggacgcactg gcagagtacc
     8641 aacgcgcggg aaaaattcgt tatatcggcg tgtcgaacga aactgcattt ggcgtaatgc
     8701 gctacctgca tctggcggac aaacacgatc tgccgagtat tgtcaccatt cagaaccctt
     8761 acagtctgtt aaaccgcagt tttgaagtag gtctggcaga agtcagccag tatgaagggg
     8821 tcgaactgct ggcctattca tgcctgggtt tcggcacgct gaccgggaaa tatctcaatg
     8881 gtgcaaaaac cgctggcgca cgtaatacgc tctttagtcg gttcacccgc tatagcggtg
     8941 agcaaacgca aaaagccgtc gcggcgtatg ttgatatcgc cagacgtcat ggcctggacc
     9001 ctgctcagat ggcgctcgcg tttgtacgcc gtcaaccgtt tgttgccagc actctgctgg
     9061 gcgcaaccac gatggatcag ctgaaaacta acatcgaaag tttgcatctg gagttaagcg
     9121 aagacgtatt agctgaaatt gaagcggtgc atcaggttta tacttatccg gcaccataag
     9181 attaacgccg gttaaaaccg gcgttaaata ttaatgacgg cgctgccaga tccacagcgc
     9241 cgttattgcc agcgcaaaca gcgcaccgaa gccaatgcca atgggcacga ccgggatgcc
     9301 taccattacc gccagcgagt aaatgcccag catcaacaac atggcgctgt tttcgccaag
     9361 gttttgtact gcaatcgcat tccccgcccc gacgcttttt ttaccccgct cctgtagcaa
     9421 cgcattgagc ggaacgacaa aaaagccccc catcacgcca atcagcatca acaaggcata
     9481 ggctggcagc agctcgtgtt gcagggaaaa aatcagtacc accacgccaa tcaaaatccc
     9541 ggctggcata cagcgtgaca cggtttccag cgtaactaac ttcgctgccg cacctgcgcc
     9601 aaccacgata ccaatcgcta ccatcgcgtt gagataggtg ggcgtagagt tatcggtaat
     9661 gcccagcgcc accggtaccc acagcaccaa caggaaacgc agcgtgacac ccgctcccca
     9721 gaataaactg gtgcccacca gcgaaaaacg cgtttcacca ttgcgccata gcgaggtgca
     9781 ggcattcagg aaactgcggg tcatgttgat gagattccaa gactgccccg gacgcgccgc
     9841 cgccagtttg ggaatgtaga tattggcaac gaccgcacca ccgtaggcca gtgcgcatgc
     9901 ggccagggcg acgaggacat gccagtcagc cagcacacca ccggctacgg aaccgagcaa
     9961 aatcgccgct atggtagaag cttccattaa accgttagct ttcactaact tactacccgt
    10021 ggttaattcg ccgagaatac cgtatttcgc cggtgaatag gctgcagcac caacccccac
    10081 cagcgtatag ccgagaaacg gattgatacc aaagcagata ctggctgcgc ccagcagctt
    10141 caggccgttg gcaaacatca tcacccggcc tttggcgaag ctatccgcca cctgcccgac
    10201 aaacggcgca aaaagaatgt aagcacctac aaacaccatt tgcaggatgg gctggctcca
    10261 ctccggatag aactgcgctt tcagtaacgc cagagtggca aacagtaggg cattatcgcc
    10321 aaacgcagag agaaactgcg ccacgataac cgctttcatc cccttcgacc acaacgaagt
    10381 gttagtgtgc actgactcac tcatcgtgtt gttccgcttc gtctacccag cttttcaacg
    10441 tgacaaagtc aggtttgccg ctgccaagta atggcatctg tttcagatag cgaatatcgc
    10501 gcggtacagc aagctccggc acgccgtgct cgcgggcata ctgttgcaac ttatcgagcg
    10561 tcagttcgtt atctgtggtg aaaagcacca gtgcctcgcc tttgctggca tcgctcttaa
    10621 tcgcagtggc atggacttta tctggcgaaa caccaagtgc cagttgttcc accatttcca
    10681 gcgacaccat ttcgcctgca attttggcaa agcgttttgc gcggccctga atctgcacaa
    10741 agccctgctc gtcaaaacgc acaatatcgc cagtgtcata ccagccgcgc tccatttcgc
    10801 cgcgaacatt ctcggcggtg ggcacttcca gtacacctgg cttctccacc cgcagatagc
    10861 cgttcattat gttcggccct ttcagttgca ggcgtccgcc ctcttcgata ccaggaaccg
    10921 acaacagacg cgcatccatt cctggtagaa tacgccctac cgtaccgggt ttcgccgcca
    10981 tcggtacgtt gatagaaacg acaggcgcgc actcggttac accatagcct tcaaggatgc
    11041 gcaggccaaa tttatcctgc caaagctgtt tggtactttc ttgtagtttt tctgcgcctg
    11101 ccaccacata gcgtagacga tagaagtcat acgggttggc gaaacgcgcg tagtgaccga
    11161 ggaaagtcga ggtaccgaac aacacggtgc aactgcggtc atacaccaac tccggcacaa
    11221 tgcggtaatg cagcgggctt ggataaagga acacttctgc acctgtaagc agtggcgtaa
    11281 acaggcctac cgtcagccca aaggagtgaa acagcggtaa tgccgacata aagcgatcgt
    11341 tggtggtgaa gtcggcaatc gttttaatct gctcgacgtt cgccagaatg cttttatggc
    11401 tatggacgac gccttttgga tggccttcag aaccggaggt aaagaggatc agcgcctctt
    11461 cttccggctg ctgttttacc tgcgccaggc gcggcatcag caaatgagcg aagatccata
    11521 ctttgtcggc agtggtgaca tctgctttta aatcttccag atagacccag cgcacctgag
    11581 taagttgctc cggcagatgc cagagtttgc ctttatcgag aaactggcgg gaagtgaaga
    11641 tggttttgat ttcagccgcc gtaatagcac tggtcagccc ttttacccca gcagtgtagt
    11701 tcatcattgc gggaatgcgg cgacgcgcga tggccccaaa aatcactgcc gcactgatgc
    11761 ctgcattggg cagcattaag ccgatgcgtt cgccttcaac actgtatttt tcaaggatgc
    11821 gtccaacaaa cagcgttttc gtaagcaatt tgcgatagga gtctggggta aagttgacgt
    11881 cttcgacaca tttcttcccg gctccgaagc ggtacattgc actcagtaaa gattcgtaca
    11941 gagtttcacg cgggcgcacc gccatacgcg cttccatcat tatttgatgc agcatttcgc
    12001 cagcgatttt gcgacggtca cgggcacgcg acgcatccgg catcgccacc tgcgttggag
    12061 gcaaaatatg cagagtaatt tgcgggaaca agcgacgttt aaccagacct ttcaggcggc
    12121 tgaagtgcgt aagttccgcc ccttcaatac gcacaggaat aaccgttgca ccagacttcg
    12181 ccgcgacaaa acccgcgcca tcgtagattt tcatcagcga gcctgtagtg gtgatgcgtc
    12241 cttcagggaa aatcaccact ggtcggccct gttccaccag acgtaccaga tgtttaatag
    12301 ccataggttg cgtcgggtcg agaggaacaa agtcgataaa tgatttcagc caacgcatat
    12361 accactgttg gcttattgag gtgtaaacgg caaacactgg acgcacaggt aaaaacagtc
    12421 caagcaaaat gccatcaata aaagagacgt gattaggcgt aattagaacg cgctcgccct
    12481 tcagtgcctg ggtgtcaccc gtaacgcgaa cgcgatacaa aacacggcac aaatttcgaa
    12541 aaaagctaaa aagcattcca actccctttg ctctgattca gtaaaagcga atggagggag
    12601 attacacgag ataaagaacg cgagcgacag taaattaggt gcgaaaaaaa acctgcgcat
    12661 ccgcgcaggt tggtgcaaga gacagggtac gaagagcgta ccgaataatc tcaccaatca
    12721 atacctctgg gatcaccact ttagcaacct gaagccaaac gccaccagcg gtcaatcgca
    12781 acagcgtttc gcaaagtgta accaaaggtt tgcaatctct ttttttgtct tacttatttg
    12841 cgtttggctc acattcccac gatgaaaaca cgccactcct tgaaccaacg ggcgttttcc
    12901 gtaacactga aagaatgtaa gcgtttaccc actaaggtat tttcatggcg accataaagg
    12961 atgtagcccg actggcaggc gtttcagtcg ccaccgtttc ccgcgtcatt aataattcac
    13021 ccaaagccag cgaagcttcc cggctggctg tgcatagtgc aatggagtct cttagctatc
    13081 acccgaacgc caacgcccgt gcgctggcgc agcagaccac tgaaacggtc ggtctgatcg
    13141 ttggtgatgt ttccgatccg tttttcggtg caatggtgaa agcggtcgaa caggtggctt
    13201 atcacaccgg taatttctta ttgattggca acggttacca caacgaacaa aaagagcgtc
    13261 aggctattga gcaactgatc cgccatcgct gtgctgcgtt ggtcgtccat gccaaaatga
    13321 tcccggatgc cgatttagcc tcattaatga aacaaatgcc cggtatggtg ctgatcaacc
    13381 gtatcctgcc tggctttgaa aaccgttgta ttgctcttga cgatcgttac ggtgcctggc
    13441 tggcaacgcg tcatttaatt cagcaaggtc atacccgcat tggttatctg tgctctaacc
    13501 actctatttc tgacgccgaa gatcgtctgc aagggtatta cgatgccctt gctgaaagtg
    13561 gtattgcggc caatgaccgg ctggtgacat ttggcgaacc agacgaaagc ggcggcgaac
    13621 aggcaatgac cgagcttttg ggacgaggaa gaaatttcac tgcggtagcc tgttataacg
    13681 attcaatggc ggcgggtgcg atgggcgttc tcaatgataa tggtattgat gtaccgggtg
    13741 agatttcgtt aattggcttt gatgatgtgc tggtgtcacg ctatgtgcgt ccgcgcctga
    13801 ccaccgtgcg ttacccaatc gtgacgatgg cgacccaggc tgccgaactg gctttggcgc
    13861 tggcggataa tcgccctctc ccggaaatca ctaatgtctt tagtccgacg ctggtacgtc
    13921 gtcattcagt gtcaactccg tcgctggagg caagtcatca tgcaaccagc gactaaccgc
    13981 agttaaagca attccagcgc cagtaattct tcgatggtct ggcgacggcg aatcaaccgc
    14041 gcctgaccat tatcaaacag aacttctggt aacagcgaac ggctattgta gttggatgac
    14101 attgatgcgc catatgcccc tgtatcatgc agtaccagat aatcacctgc cttcacttcc
    14161 ggcaaggcgc gggtttcaac atttccccct tcctgctggg taaagacatc gcccgattca
    14221 cataacggtc cggcgacgac ggtttccacc gttggcgcgt gttccagaga acgaccatca
    14281 gctgccaggg cactgatatg gtggtaacta ccgtacattg ccgggcgcat cagatcgttg
    14341 aacccggcat caaccagcac aaagtggcgg ctccccattt gtttgacgct ccgcacctga
    14401 gtaattaata cgccagactg cgctaccagg aagcgacccg gttcaatttc cagtttcaca
    14461 gggtggccca aatggcgggc gatttgctca cgcgcggcat tccacagacc ataataatgt
    14521 tcggtatcaa ccgcctcttc accctgttga taaggaacag aaagcccacc gcccgcagaa
    14581 atagcctgta aatcctgacc gaattcgatg acctgacgca ccatagcacc acacacctgt
    14641 tccagatggg cataatcaac gccagaacca atgtgcatgt gaatgccgac cagctgcaga
    14701 tgatgacgtt gtatcacgtc cagtgcggcg ggcagatcgg tgtaccagat accgtgcttg
    14761 ctgttttcgc caccggtatt ggttttttgg ctatgtccgt gaccaaaccc cggattaacg
    14821 cgcagccata cccgatgccc tggcgaaacc tggcccagtt ggtcgagcat atcaacagaa
    14881 cccgcattca ccggaatttg caattcactg acgcgttcaa gcgtcgcctg atcgataaca
    14941 tctgccgtaa aaacaatatc atcggggtgc gtttgcggat tgtaacccgc cgccaacgca
    15001 cgctctattt cgcctaacga gacggaatcc actttcacgc cctgctcacg cattaagcgc
    15061 aaaatatgaa tattggaaca ggctttctgt gcaaagcgca ccacatcaaa ctgtttcagc
    15121 gctgcaatct gccgacgaat aatttgcgca tcgtagaccc acaccgggca gccaaattca
    15181 gcgggcaaac gcagcagatt ttcggcggtg agatcggtat cggtgctgaa cagtgaatgt
    15241 ggcataacaa actccagata agtgcttttt tatgattacg ccacatcata aaaagaataa
    15301 aaaatatcga tttatgtcga gtctatgcaa aaatgatatg gattaccgga ttgcgagaga
    15361 gcgctaatgg ccgccgttaa cttacgtcat attgaaattt ttcatgcggt aatgaccgcc
    15421 ggaagcctga ctgaggcggc acacctgcta cacacctcac agccaaccgt cagccgcgaa
    15481 cttgcgcgct ttgagaagat gatcgggctg aaattgtttg agcgcgtacg tgggcgatta
    15541 catcctaccg tgcaaggact gcgtctgttt gaagaagtgc aacgatcctg gtacggactg
    15601 gatcgaattg tcagcgctgc agaaagtctg cgcgagtttc gccagggaga actgtctatt
    15661 gcctgcctgc cggtcttttc gcaatctttt ttaccgcagc tcctgcaacc ctttctggca
    15721 cgttatcccg atgtcagctt aaatatcgtg ccccaggaat caccgctact tgaagagtgg
    15781 ctctcggccc agcgtcatga tttaggactc actgagacac tccatacgcc tgcgggaaca
    15841 gaacgtaccg aattactctc tttagatgaa gtgtgtgtgt tacctccggg tcatccgctg
    15901 gcggtaaaaa aggtattaac gccggatgat tttcagggtg agaactacat cagcctttcc
    15961 cgtactgaca gctatcgcca gttgctggat cagctattta ctgaacatca ggttaaacga
    16021 cgcatgatcg tagaaaccca cagcgccgcg tcaatctgcg caatggtacg ggcgggggta
    16081 ggtgtttcgg tggttaaccc gctcaccgca ctggattatg cggcaagcgg tttagtggtg
    16141 cggcggttca gtattgcggt tccgttcaca gtcagcctga tccgccccct gcaccgcccg
    16201 tcatcagcgc tggtgcaggc gtttagtgag catttacaag cggggttacc gaaactggtc
    16261 acttctcttg acgctatttt gtcgtcagct acgacagcat aaaagcgaca gcatcctcgg
    16321 catggatcgc cgcagtatca aacacaggca aaacactgcg ctcttctggc accagtaaac
    16381 caatttctgt gcagccaaaa atgacgccct gtgcgccctg ttctgcaagg cgagcaatca
    16441 cttgcgcata ataagcgcgt gacgcttcgg taaattgccc cagacacagt tcttcaaaaa
    16501 taatctgatt aattttcgcc cgttcatccg cttcaggaat aagacaattg atggaaaatt
    16561 gttccgtcag ccgcccgcga taaaaatcct gttccatggt gtaacgcgta cccagcagcg
    16621 ccacacgagt cattccggcc ccggtaattg cacgtccggt ggcatccgca atgtgtaaga
    16681 aaggcagagt gcaacgtgac tcaatggcat ccgccacttt atgcatcgta ttggtacata
    16741 acacaatacc ttctgcgccc gcccgctgta agccaagcgc cgcctcagcc agaatgtccc
    16801 cggttttatc ccattccccg cgacgctggc actcttctat ttcatgaaaa tcgacgctat
    16861 gtagcagcac ttgcgcagag tgaagcccac caagccgctg tttaatgcct tcatttatca
    16921 aacgatagta aggaatggtg gattcccagc tcattcctcc cagcaaacca attgttttca
    16981 ttcctctctc ctgatgtgtg ttaccgcaac aaggctgaat aaggaacgag acaaacgcct
    17041 caacggccaa gtgcccaatc tctattaacg aaaaaagggc cggatgtaca gcacatccgg
    17101 cccgtgaaat cagatgccga tatttctcaa cttctcgcct gccatcagtt tgcgttcgat
    17161 atgttccagc gtgacatttt tggtttccgg aatgagccag aaagtgatgc ccacaaacgc
    17221 aatgttcagc gcagtgtaga gccagaacgt accggcagcg ccaatgctgt cgagcagcgt
    17281 caggaaggtc gccgataatc atattcgaca cccagttcgt ggtggtcgaa caggtaatac
    17341 cgaaatcgcg gcatttcagc ggctgaattt cagagcacag gatccacacc actggcgcgg
    17401 cgctcatcgc ataaccggca atacacatca tcgtcatgcc aacagagagc caggacaatc
    17461 cactggaagc cgtaccgtta tcaaactgca tcaggcaata gcccagcacc agagtgccta
    17521 acgccatcac gctgaaacca attttcagag ccggtttacc cctgctttat ctaccgtaaa
    17581 caccgcaata aaggtggcga acataaaggt cagccctacg accagagtcg caatcatctg
    17641 ttgttctgtg gtcgtaaagc ccgccatttt gaagatacgc ggcgcgtagt acatgatgat
    17701 gttcataccg gtaaactgct gcatcgcctg caacaacata ccgagaaaca cagcacgacg
    17761 gacgttacgg ttgatcttaa acagtgccca accgccctgt tttaacttca ggctttcacg
    17821 aatttcgttg agttcttctc gcgctttttc cgacgtatcg cgcagcatac gcaatacttc
    17881 ttccgcctca atatgacgcc ccttttccgc caaccagcgc gggctatttg gcaggaagac
    17941 caccagaata atcagcagaa ctgctggtaa agcaagaacc cccaacattg cgcgccagtt
    18001 accgctataa ctgaacgctg tatcggataa aaacgccagc acgatgccga gtgtgaccat
    18061 caactggtac atactgatca tcttaccgcg aacgttttca cttgccattt cagaaagata
    18121 cagaggagcg gtgtaagacg cgatcccgac agcaatgccc agccccacac gagcggcgat
    18181 taacatctct acgctggtcg caaaagcgga ccctatagaa ccgagtacaa acaggatggc
    18241 ccccgccatc aggctgtatt tacgccccag gcggaacgac agccaaccat taaacagcgc
    18301 accaattgct gcgccgagca tcatgctact aaccacccat tcctgcaaat gactggtcag
    18361 cacaaagtga tcggtaatga acggcaacgc tccggcgatt acgccgatat caagaccaaa
    18421 taacaatcct gcgaccgcag cagctaccga aacaaacata ttcatacgcc gcgtatcccg
    18481 caaagaacgt ggcgttaaag cagattccgt attgatagta accatttttt cctgccagca
    18541 gagagtaaga catagtgaaa aaatacgtga acaactcacg caggtgtcag gtcggaaaca
    18601 gcataaatat ggattaaatt gctgcgacat gtcgttatgt gatggatatt ccaattttca
    18661 aattaagttg aattattgag attattatta accacctaat tttacagcag ataaaattca
    18721 taaagttcat taattgataa ttaatatgga ttatttcata accatgatat ggattatgat
    18781 gatctacagg tataaaaaac cctgccatgc ggcagggtca taaaagtaag aagaatgaat
    18841 taacgcgcca gccaaccgcc atccacggca atggtataac cattcacata atctgaagcg
    18901 ctggaggcaa ggaacactat cggccccatc aggtcactcg gcagtcccca acgaccagct
    18961 ggaatgcggt cgagaatttc cgcgctacgt tgttcatctg cccgtagttg ttgagtattg
    19021 ttggtcgcca tgtaacccgg agctatcgca ttaacattaa tgttgtgttt agcccattcg
    19081 ttcgccatca atcgcgtcac acccatcacg ccgctttttg atgcggtata agaaggcaca
    19141 cggatcccgc cctggaagga gagcattgac gcgatattga taatcttgcc gccattgcct
    19201 tgcgcgataa agtgtttcgc cgctgcctga gacatgaaga atacgctctt gatattcagg
    19261 ttcatgacat cgtcccagtc tttttcgctg aactcgagag cgtcttcgcg gcgaatcaat
    19321 ccggcgttat tcaccaggat atcaatatga ccaaattcag ctaccgcgcg atccagcagt
    19381 ccaggaatgc catcaatctt tcgcagatcg gcggtcaggc ttaaaaaacg acgccccagc
    19441 gccgtgacct gctcgatggt ttcagtcggt tcaacgatgt taatgccaac aatgtcacag
    19501 cccgcttgcg ccagccccaa cgccatcccc tggcccagcc cagtatcaca accagtgact
    19561 accgcaactt taccttcgag agaaaatgca cttaaaatca taacaatacc ttattcgttt
    19621 atgcccacaa ctagcgcaaa tctttaacgg ccacatggtc catatcatca aagacctggt
    19681 tttcaccaac catgccccag ataaaggtat aagctttggt tccgacgccg gaatggatcg
    19741 accagctcgg ggagatcacc gcctgctcgt tatgcatcac aatgtgacgc gtttcttgcg
    19801 gttgccccat catgtggaaa acgcaggcgt catcatccat attgaaatag aaataaactt
    19861 ccatccggcg ctcgtgggtg tgacacggca tggtgttcca caagttacct ggagccagct
    19921 ccgtcagccc catactcaat tggcaggttt ccagcacatc cgggacaaaa tatttgttaa
    19981 tagtgcgacg gttactggtg aggttatcgc ctaacgtgac tggagatact tcgtccgggg
    20041 tgactttttt ggtgggatac gtcgtatgcg cgggtgcgca attgtaataa aactttgccg
    20101 gagtggcggt atcaatactg gcaaagacaa cttcttttgc acctttacca acatacaggg
    20161 cgtcgcggtg accgatttca tagcattggc catcgacagt aatcgtaccg gcaccgccaa
    20221 tattgataac acctaactcg cgacgttcaa ggaaatagct tacgccgagt tgtttaccaa
    20281 cttccccgcc aacggaaacc gttttagtta tcggcataat gccgccaaca ataattcggt
    20341 caatgtggct gtaaaccatg gtgtactcat cggcgacaaa taccttttca accaaaaatt
    20401 cattgtgcag cccttgggta tccagcgttt ttgcgtgcgc actgtggatg ctctgtctta
    20461 cgtccacatc aacctccgat aatcagtgaa gcgtgaaaat agaaaacgat agtgaaacac
    20521 tgtttcgttt tctctataaa gggatgatat gcccatgggc tagggttttc aattaaaaat
    20581 aaaacaatgt tttattttta agttaaggat taaaaaaagt gcagtgtcga tcacgaatat
    20641 gtcatgagaa tggaaaaatg agggagccca gaaaatgtat gtcgacttgc cagccatcat
    20701 tattcaacag attaatgacg gcagatattt atcggtaagg aggaaatgca gagctactcg
    20761 tcacgttcaa tggtcaatgc cacgccctgg cccccgccga tacaaagcgt tgccagccct
    20821 ttgcgggcat tacgtttcac catttcatga accagagaaa ccaggattcg gcaaccggaa
    20881 gcgcctatcg ggtgaccgag tgcgatcgcg ccaccattga cattgacccg acgctcatcc
    20941 cactcaagca tcttgccaac cgaaagcgcc tgtgcagcaa acgcttcatt agcctcgata
    21001 agatcgacat cagccaactg ccagcctaca cgctccaggc aacggcgggt cgcatacacc
    21061 ggcgcaattc ccatcaatgc cggatctaca ccaacgctgg caaatgcgcg gatgcgggcc
    21121 agcacgggta aattcaacgc tcgtgctttg gcttcgctca tcatcattac cgcagctgcg
    21181 ccatcgttta tggatgatgc attccccgcg gtcacagaac cgagactatc aaatgaagga
    21241 tttaaacggg ctaaaccttc tgcactggcg tcagtgcatg gctgttcatc ggtatcaaca
    21301 accaacgtct gaccgttact ttggatcatt accgggacga tctcatcttt aaatcgtccg
    21361 gcgtcaatcg ccgctcgcgc tttttgttgc gagctaagtg cgtaagcatc ctgcaactga
    21421 cggctgatgc catattcgcg agccagattt tcggcggtga caccaatatg ataatcattg
    21481 aaggcatccc acaacccatc atgcaccaga ctgtcaacca actggctatt gccaagctgt
    21541 gcaccggtgc ggctatcagt cagaacatgt ggtgcgcggc tcatgttttc ctggccaccg
    21601 gcgatgacaa tatcagcctc gccacactgt atcgcctgag tagccagatg cagtgcttta
    21661 agcccagaac cgcaaacgtc attaatagtg attgcagaaa cgctattagg aagaccacct
    21721 ttaatagccg attgccttgc cggattctgc cctgcccctg cagtcaacac ctgaccaaga
    21781 attacttcat ccaccgcata tgcaggaacg ccggtacgtt ctattaacgc tttcacgacc
    21841 aaactaccaa gttccacggc ggaatgaccc gctaacgcac cacgaaagca gccgataggt
    21901 gtccgtaacg ccccgacaat cacaacgtct ttcatcacaa cctcgcacca attaacagtg
    21961 caatagtaaa tgattgttaa ttacagttat cttaattgtt taaaaaaagt gatttttatc
    22021 acaaaagaaa tatgcctgag cagcagtcag agacataact ggcacgtaag gtttgcaacc
    22081 actaacccac caatagaggg gtagataggg cgttaatctc ccatacttaa cctggtttat
    22141 ggtaaattgc cctccatttt gtttaatttg tagatgatac gttcagataa tgtctaatat
    22201 ttggtctaaa gaagaaactc tgtggagttt cgcgctctac ggcacagccg ttggtgcagg
    22261 cacgctcttc cttcctattc agttaggttc ggcaggggct gtggtcctgt ttattactgc
    22321 tctggtcgcc tggcctttaa catattggcc acataaagct ttatgccagt tcatcctctc
    22381 atcgaaaaca tcagcaggtg aagggataac gggcgcggta acacactact atggcaagaa
    22441 gattggtaat ctgattacca cgctgtactt catcgccttt tttgtcgtcg tgttgatata
    22501 tgcagtggca attaccaact cacttacaga acagctggca aagcatatgg ttattgatct
    22561 tcgcatccgt atgttggtga gtctgggt
//
LOCUS       DAZPEB010000050        22122 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZPEB010000050 DAZPEB010000000
VERSION     DAZPEB010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22122
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.051"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..1298
                     /locus_tag="ACHURJ_001679"
     CDS             <1..1298
                     /locus_tag="ACHURJ_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFR1280666.1"
                     /translation="WLELEGAKDLDDALSLLTILYHHVPSVTSMPVYLGQLDALLQPY
                     VRILTQDEIDVRIKRFWRYLDGTLPDAFMHANIGPSDSPITRAILRADAELKQVSPNL
                     TFIYDPEIPPDDLLLEVAKNICECSKPHIANGPVHDKIFTKGGYGIVSCYNSLPLAGG
                     GSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQIAIIDARCEFLYQQSHFFENSFLV
                     KEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAARYGKEAAANEVGYRISAQLAEFVA
                     NTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPYGDEPDPIIHLQTVAPHHAYYYSGI
                     SDILTLDETIKRNPQALVQLCLGAFKAGMREFTANVSGNDLVRVTGYMVRLSDLEKYR
                     AEGSRTNTTWLGEEATRNTRILERQPRVISHEQQMRFSQ"
     gene            1270..2133
                     /locus_tag="ACHURJ_001680"
     CDS             1270..2133
                     /locus_tag="ACHURJ_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFR1280667.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVLWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQHLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(2347..3129)
                     /locus_tag="ACHURJ_001681"
     CDS             complement(2347..3129)
                     /locus_tag="ACHURJ_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR1280668.1"
                     /translation="MICRFIDTHCHFDFLPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEVGLDL
                     FGDDPQLERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLSRTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
     gene            complement(3126..4199)
                     /locus_tag="ACHURJ_001682"
     CDS             complement(3126..4199)
                     /locus_tag="ACHURJ_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFR1280669.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGCYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVIDAPQANDPTFNNEDLA"
     gene            complement(4321..4482)
                     /locus_tag="ACHURJ_001683"
     CDS             complement(4321..4482)
                     /locus_tag="ACHURJ_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFR1280670.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(4609..5214)
                     /gene="osmY"
                     /locus_tag="ACHURJ_001684"
                     /pseudo
     CDS             complement(4609..5214)
                     /gene="osmY"
                     /locus_tag="ACHURJ_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
     gene            complement(5607..7196)
                     /gene="prfC"
                     /locus_tag="ACHURJ_001685"
     CDS             complement(5607..7196)
                     /gene="prfC"
                     /locus_tag="ACHURJ_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFR1280671.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKLTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(7287..7964)
                     /gene="yjjG"
                     /locus_tag="ACHURJ_001686"
     CDS             complement(7287..7964)
                     /gene="yjjG"
                     /locus_tag="ACHURJ_001686"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFR1280672.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGIAKPNKKIFDYAL
                     ELAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHLEQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(7979..8425)
                     /gene="rimI"
                     /locus_tag="ACHURJ_001687"
     CDS             complement(7979..8425)
                     /gene="rimI"
                     /locus_tag="ACHURJ_001687"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFR1280673.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKNFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIVVDPDYQRQGLGRVLLKHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(8394..8807)
                     /gene="holD"
                     /locus_tag="ACHURJ_001688"
     CDS             complement(8394..8807)
                     /gene="holD"
                     /locus_tag="ACHURJ_001688"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFR1280674.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            8910..9941
                     /gene="rsmC"
                     /locus_tag="ACHURJ_001689"
     CDS             8910..9941
                     /gene="rsmC"
                     /locus_tag="ACHURJ_001689"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFR1280675.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATVDDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            10209..10295
                     /locus_tag="ACHURJ_001690"
     tRNA            10209..10295
                     /locus_tag="ACHURJ_001690"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10243..10245,aa:Leu,seq:cag)
     gene            complement(10334..10570)
                     /locus_tag="ACHURJ_001691"
     CDS             complement(10334..10570)
                     /locus_tag="ACHURJ_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFR1280676.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAAIMLMIMNLNQG"
     gene            10711..11499
                     /gene="fhuF"
                     /locus_tag="ACHURJ_001692"
     CDS             10711..11499
                     /gene="fhuF"
                     /locus_tag="ACHURJ_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFR1280677.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(11537..12213)
                     /gene="bglJ"
                     /locus_tag="ACHURJ_001693"
                     /pseudo
     CDS             complement(11537..12213)
                     /gene="bglJ"
                     /locus_tag="ACHURJ_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
     gene            complement(12171..12896)
                     /locus_tag="ACHURJ_001694"
     CDS             complement(12171..12896)
                     /locus_tag="ACHURJ_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1280678.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDF
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMNETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIVSLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            13515..14285
                     /locus_tag="ACHURJ_001695"
     CDS             13515..14285
                     /locus_tag="ACHURJ_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFR1280679.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAIITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            14276..14749
                     /locus_tag="ACHURJ_001696"
     CDS             14276..14749
                     /locus_tag="ACHURJ_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFR1280680.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTRGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            14857..15396
                     /gene="dnaT"
                     /locus_tag="ACHURJ_001697"
     CDS             14857..15396
                     /gene="dnaT"
                     /locus_tag="ACHURJ_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFR1280681.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            15399..16136
                     /gene="dnaC"
                     /locus_tag="ACHURJ_001698"
     CDS             15399..16136
                     /gene="dnaC"
                     /locus_tag="ACHURJ_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFR1280682.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            16185..16679
                     /gene="yjjA"
                     /locus_tag="ACHURJ_001699"
     CDS             16185..16679
                     /gene="yjjA"
                     /locus_tag="ACHURJ_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFR1280683.1"
                     /translation="MMETVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            16933..19224
                     /gene="opgB"
                     /locus_tag="ACHURJ_001700"
     CDS             16933..19224
                     /gene="opgB"
                     /locus_tag="ACHURJ_001700"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFR1280684.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRHRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(19363..20385)
                     /gene="lgoD"
                     /locus_tag="ACHURJ_001701"
     CDS             complement(19363..20385)
                     /gene="lgoD"
                     /locus_tag="ACHURJ_001701"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HFR1280685.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVRLGKNIADLKNGLQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            20524..21438
                     /locus_tag="ACHURJ_001702"
     CDS             20524..21438
                     /locus_tag="ACHURJ_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFR1280686.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTLINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            21653..>22122
                     /locus_tag="ACHURJ_001703"
     CDS             21653..>22122
                     /locus_tag="ACHURJ_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1280687.1"
                     /translation="MEKENITIDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHNFTQFVLVRIGMGIGEAPMNPCGVKVIN"
BASE COUNT         5469 a         5788 c         5459 g         5406 t
ORIGIN      
        1 aatggctgga gctggaaggc gcgaaagatc ttgatgacgc actctctctg ctgaccattc
       61 tttaccacca cgtaccgtcg gtcacatcga tgccggtcta cctagggcaa ctggatgcgt
      121 tgttgcaacc gtatgttaga attctaacac aagacgagat cgatgttcga ataaaacgtt
      181 tctggcgtta cctcgacgga accctgccag acgcctttat gcacgccaat atcggcccgt
      241 ctgattcgcc cattacccgc gcgatcttac gtgcagacgc agagttgaag caggtttcac
      301 cgaacctgac ctttatctac gatcctgaaa tcccccctga tgacctgctg ctggaagtgg
      361 cgaagaacat ctgtgaatgt agcaaaccgc acatcgccaa cggtccggtg catgataaaa
      421 ttttcacaaa agggggctac gggattgtga gctgttacaa ctcactgccg ctggcgggtg
      481 gtggcagcac gctggtacgc cttaacctga aagccattgc cgagcgcagc gaatcgctgg
      541 atgacttctt tacgcgcact ctaccgcact actgccagca gcagatcgcc atcatcgatg
      601 cgcggtgtga attcctctat caacaatcac acttctttga gaatagcttc ctggtgaaag
      661 aagggctgat taaccctgaa cgttttgtgc caatgtttgg catgtatggg ctggcggaag
      721 cggttaactt gctgtgtgag aaagaaggga ttgccgcgcg ctacggtaaa gaagccgccg
      781 caaatgaagt gggttatcgc atcagcgcgc aactggcgga gtttgtcgcc aatacccccg
      841 tgaaatatgg ctggcaaaaa cgcgccatgt tacacgcaca gtcggggatc agttccgata
      901 tcggcaccac gccgggcgcg cgtttgccgt atggcgatga gccagatccg atcatccatc
      961 tgcaaactgt cgccccgcat catgcttatt attattccgg catcagcgac attctgacgc
     1021 tcgacgaaac catcaaacgt aatccgcagg cgctggtaca gctttgcctc ggtgccttta
     1081 aagccggaat gcgtgaattt accgccaatg tcagcggtaa cgatctggtt cgcgttaccg
     1141 gttatatggt gcgtttgtcg gatttagaaa aatatcgcgc cgaaggttca cgcaccaaca
     1201 ccacctggct gggcgaagaa gccacacgca acactcgtat tctggaacgc cagccgcgcg
     1261 tgataagcca tgaacagcag atgcgcttta gtcagtaaga ttatcccctt ctcctgcgtt
     1321 gacgggccag gcagtcgtct ggctctgttt ctgcagggct gcaatctgcg ctgcaaaaac
     1381 tgtcacaatc cgtggacgat gggacgctgc aatgattgcg gagagtgcgt gccacagtgt
     1441 ccgcatcagg cgttgcagat tgttgacggc aaagtgctgt ggaacgctgt ggtctgcgag
     1501 cagtgtgata cctgcctgaa gatgtgtccg caacatgcca cgccaatggc gcaatccatg
     1561 agcgtggacg aagtgcttag ccatgtccgc aaagcggtgc tgtttattga agggataacg
     1621 gtaagcggcg gcgaagccac gacccaactg ccgtttgtag tggcgctgtt tactgctatc
     1681 aaaaacgatc cgcaactgcg ccacctcacc tgcctggtgg acagtaacgg catgttgagc
     1741 gaaaccggct gggaaaaatt actcccggtg tgtgacggcg caatgctcga tctcaaagcg
     1801 tgggggagcg aatgtcatca acacttaacc ggacgcgata atcagcagat taagcgcagc
     1861 atctgtttgc ttgcagagcg cggcaagctg gcggaactgc gtttgctggt aattccagac
     1921 caggtggatt atttgcatca catcgatgag ctggcgacgt ttatcaagag acttggcgat
     1981 gttccggttc gcctgaatgc gtttcatgcc cacggcgtgt atggcgaggc gcaaagctgg
     2041 gcgagcgcca cgccggaaga cgttgagccg ttggctgatg cgttaaaggt gcgcggggtg
     2101 agccggttga tatttccggc gctctatttg tgacatgccg gatacggcct acggttcggc
     2161 acagacttgt aggcatgata agacgcgaca agcgtcgcat caggcattgt gcgccaactg
     2221 ccggatgcgg cgtgaacgcc ttatccggcc tacggttcgg cacaaacctg taggcatgat
     2281 aagacgcgac aagcgtcgca tcaggcatta tgcgccgact gccggatact gcctacgaag
     2341 ggtaaactac ccactaacgc taaacaacgc atacgtatta ttaagcaaca cttcggcaat
     2401 ctcatcttct ggttccgggc gcaactcgca aagcacgtca aacacccgca cagcctgttc
     2461 cggtcggtta ggctgcccct gaaagccgtt gagcggcata tccggcgcgt cggtttccag
     2521 caacaacgat gccagcggta attttgcgat gacatcgcgg gttttactgg cgcgtggata
     2581 ggtgatagta ccgcctacgc cgattttgta gcccagctgt acaaaccgct cggcctgttg
     2641 caggctgccg gaaaaaccgt gaaccacgcc agtgcgcgat aaatcgtggc gtttaagatg
     2701 catcgccagt ttgtcgtgtg tgcgccgtga atgcaggatc accggcagat cgtagcgttt
     2761 cgccagtttc agctgttcgt cgagtaacca ctgctgcctc tcaagttgcg gatcgtcgcc
     2821 gaagagatcc agaccgacct cccccactgc caccaccttc gccggacgcc tttccagcgc
     2881 ctgctgtagc tgctcaagag acacatcgct atgtttttcc aacatacctg gatgcaagcc
     2941 caatgcggca tacagcggtt gatatttttc cgctaacgcc tgcacacggg caaaattttc
     3001 cgcctctgtc gccggaacaa tgatcttgcc tacgcccgct tgtgccgcgc gttgcaggct
     3061 ggcctcttca tcgccactaa acggcaggaa atcaaaatgg cagtgggtgt cgataaaacg
     3121 gcaaatcaag ccagatcctc attgttaaat gtcgggtcgt tcgcctgcgg agcgtcaatc
     3181 acttcggcaa ccagcgtatc gttggcgact ggcgcaggcg gaataacgat cgattccggc
     3241 gtcaccactg gcaccagatg gcgggtaagc ggcgcttttt cagttaatag cttgccaacc
     3301 gtggcgagga aataacaacc gcataaacgc ccaagtttat agtcttcacg cagcgccgga
     3361 atccgactgc caagcgcgat actatgtaat ggcttcggcg gataaatttc gaatatccgc
     3421 agcttgcccg gtggtttctc aataaattgc tgaatgtcac gatagctggt ttcatgatgc
     3481 tgcaccagat tgaccagcgg ctgcaggcta ctgtcaccca gccagcgttc catgcgtttg
     3541 aaccactgcg gcgtgtagta catttgtgac ggcacagtgc gaatgacgac caacgtttta
     3601 gcgccctgcc ttgccgcctc tttaaccgga atcgcatcac tgatcccgcc atccaggtag
     3661 ttaatgcctt ccagcgacac tccgctacga taaaagccag gtatcgcact ggaggcgcga
     3721 atcacatcca gccagttttg tttggttggt aaaaagtaat taggcgcgta gtcatcctga
     3781 cgacaggcgc acatataaaa cgatttgccg ctgtcaaaca accgcgcggc ggtatccatt
     3841 tgcaacggca tctggcttgc ggtggcctcc accagccagt cgagatcgat aagatttcct
     3901 ccacggacaa agcgcaatgg atcgaaaaat tcgcgttttg tggtatagcg catgatgact
     3961 ttacgcgcgt aaccgggctg attgcagata aacgccgaga ggttctgcgc cccggcagat
     4021 gtgccgagat aaagatcgaa aggattaaac tgcgcgcgca taaactcatc cagcacgcca
     4081 gccgtgaaaa ttccacgctg tccgccgcct tcgcacacca gagctattcg tccaggctgg
     4141 aacggcctta gcgacaacgg cgcaatatta ccaagcgtta caggtattcg ctgccccacc
     4201 tctgccttcc tgtttttgtt gtgaaagaat gacaaagtaa cgcaatttat tattcgctaa
     4261 aaccgcaaaa gccagtccgc tggactggct ttggataaaa ttttcttaat ggtctgaaat
     4321 ctagggtcgt tttcggccca tgaacaaact caccaggaac agaataatcc cgacgacaaa
     4381 gacaatttta gctgcgcctg cagcggtacc ggccaaacca ccaaacccaa gtgcggcggc
     4441 gattaacgcg ataaccagaa atatgatgcc ccaacgaaac ataagcgtct cctttaccat
     4501 agttaatgtc accgcttgat atgagcgtga aaatcactca atgcgatatt tttagtgtgg
     4561 tgcacatcgc gcctcccgac aaagttcggg aggacgaatt acgacgaatt acttagtttt
     4621 cagatcattt ttaacgcttt tcacaccatc taccgctttg gcgatacttt cagcacggtc
     4681 actttgtgcc tgagaatcta cggtaccgga gagctgaacc acgccgtcgg tggtttcaac
     4741 tttcacatga cgggaaggga cgatatcgtc cgccagcagt ttggctttga tttcactggt
     4801 ggtggcggtg tcacccgcgt agcccttcac cgagccttct ttagcgtcgc gaacgtgcag
     4861 tttgtcgctg acagaggtca ccccttcaac gcctttcgcc actttcactg cctcttcggc
     4921 ctgagcctag ctttcaacga aaccgctcag ggtcacgact ttttgctcgg ttttgacaga
     4981 gatatcggtg ctcttgatgt tgtcatgatc caccagcgcc gccttcactt tcgcggtgat
     5041 ggcgctgtca tccatgaaat taccgacttt attcatagag ctatcgactt tttgccctgc
     5101 gctttcattg gtagtctgtg cgttgttttc cgcgtaggca gagccggtcg cgacggcaga
     5161 ggtcaacatt acagccagca gagttttcga aatcttcagt cttgtcatag tcatcgattt
     5221 attcctgtat gtttgctcgt aatttgagcc tggcaacacg aggttgcatt gctgaatagg
     5281 gagagacttc accctctaca gaagtcaatg gccgccatca caaaagcgat gggtgatgaa
     5341 taacgaccat tacagcctct gaatcagtta ttaatatcgg tagaatgaca atcgacggct
     5401 ttagatactg atatctacgc attgaacggt atttaacgcc gtcagaaatg tcattacttt
     5461 gttaaatata gatcacaatt ttgaaaccgc tcgtgatatc agcgaaaaca taagcaaaag
     5521 tgaatgtttt aagaacattc cgtaagcggc taataaggaa gggaaattga cagggcgcag
     5581 cggctaccgc gccctggagg caagaattaa tgctcgcggg tctggtggaa ctgaacgtcg
     5641 ggataacgtt cctgcgccag gcgcaggttg accatgctgg tagcgatgta agcgaggtta
     5701 tcgccgccat caagcgccag ttggctttcg ttcttacgct tgaactcttc gaatttcttc
     5761 gcgtccgcac attctaccca gcgggcagtg gcaacgttga ctgattcata tactgcttca
     5821 acgttgtatt cgctcttcag gcgcgatacc accacatcaa actgcagcac accaacagca
     5881 ccaacgatca aatcgttgtt ggagatcgga cggaacacct gcaccgcgcc ctcttcggaa
     5941 agctgtacca gccctttgag cagctgtttt tgcttcagcg gatctttcag gcggatacga
     6001 cggaacagtt ctggtgcgaa gttcggaata ccggtgaact tcatcatctc accctgggta
     6061 aaggtgtcgc cgatctgaat agtgccgtgg ttgtgcaggc cgaggatatc gcccggatac
     6121 gcttcttcaa cgtgcgaacg gtcgcccgcc ataaaggtca gcgcgtcgga gataaccaca
     6181 tctttcgcag tacgcacctg gcgcagcttc atgccttttt catatttacc ggatactacg
     6241 cgcataaacg ccacgcggtc gcgatgtttc gggtccatat tggcctgaat tttaaatacg
     6301 aagccggtta atttgtcttc gctcgcctct acggtacggg tatcagtctg acgcggcatc
     6361 ggcgcagggg cccactccac caggccatcc agcatatgat caacgccgaa gttacccagc
     6421 gcagtaccga agaataccgg agtgatttcg cccgcaagga acagttcttt gtcgaactcg
     6481 ttagacgcgc ctttcaccag ttccagttcg tcacgtagct gctgtgccag atcttcacca
     6541 accgcagcgt cgagatccgg gttattcagc cctttaacaa tacggacttc ctgaatcgtg
     6601 tggcctttac cgctctgata aagataggtt tcgtctttat aaaggtggta aacgccttta
     6661 aacagcttgc cgcagccaat cggccaggtg atcggtgcgc agccaatttt cagctcgttc
     6721 tcaacttcat cgagcagttc catcgggtcg cggatatcac ggtcaagttt gttcataaag
     6781 gtgaggatcg gcgtgtcgcg cagacgggta acttccatca gcttacgggt acgatcttca
     6841 acaccttttg cagcgtcgat aaccatcagg cagcagtcca ccgctgtcag ggtacgatag
     6901 gtatcttccg agaagtcttc gtgccccggg gtgtctagca ggttaaccag gcaatcgtga
     6961 tacggaaact gcatcacaga cgtagtaatg gagatcccac gctgcttttc catctccatc
     7021 cagtccgact tagcgtgctg gttggaacca cggcctttta ctgtaccagc ggtctgaatg
     7081 gcctgtccga acagcagcac cttctcggtg atggtagttt taccggcgtc cgggtgagaa
     7141 ataatggcaa aagtgcggcg cttcgccacc tcttgcaaat aaggagacaa cgtcataatt
     7201 ctttcttctt gagtaagcgg catcgcgccg cgcttgttga aaacgaaaaa ttgcggctat
     7261 tttacccatc aacggggggg aggcaatcag tgtttacaca ggagctgctc cagttcgtgc
     7321 aacgatgaaa cggtccaggt gggcgcgatg ccttctggtt gctcgagatg gtgcgcattc
     7381 agccagcaag tcgcaagccc ggcgttgatg ccaccgagaa tatcggactc ggcagtgtcg
     7441 ccaaccatca gcacgcgtga acggtcagga ttgcccgcca gttccagcgc ataatcgaaa
     7501 attttcttat tcggtttggc aatgccaact tcttcggaaa tcaccagcaa atcaaagtaa
     7561 tcacgcaggc ccgtccgttc cagacgcact tgctgcaagg cgctaaaacc gttggtgatg
     7621 atgccgattt tagcgttgcc acgaatggcg ttaagcagag aaaccgcgcc cggcagcggc
     7681 gtgcagattt ccgccatcgc attaataaag gcatcgttaa gcaagcctgc ttcaactttc
     7741 agccgttcgg cccagctctc gaaacgcccg tgctgaagct gtaatgaagt gatcgcgccg
     7801 ttttgataat ccacccacag tggcttgtta acggcctgat agtcctgaaa atcttcagcg
     7861 gtaaaggtga cgctgtaatc aagaaacatc cgctgcaggc cggtgaatga gtcaaaggta
     7921 aacagcgttt catcggcatc aaagaaaatc cagtcccact tcattattcc accttgtctt
     7981 acatactgat tggcaacgcc atgatgatgg cgtcttcgcg accgtccgtg gtggggtagt
     8041 aattgcggcg aatcgtcgcc tcgttaaagc ctaaactttc gtacagggca atggcggcag
     8101 cgtttgaagc acggacttcc agccatagtg tcgccacgcc gcgtttttcc agttcgtcga
     8161 tcagatgttt cagcagcacc cttcccaatc cctgacgctg atagtcagga tcgaccacaa
     8221 tattgaacaa tgtagcttca tccagcacca cttgcgtaat agcaaacgcc gccattttgc
     8281 cgttttgcgt taactgaaag ttgagataac gctcgccctg gttgctggca aagttttttt
     8341 cactccacgg aaaggcgtgg gcgcgttgtt caatgtggta agccgctggt aaatcagtcg
     8401 tttcgaggga agaaatcgtg ttcatatgtg caaatctgtt gccataacgc ggcgcgtgcc
     8461 gttgggtttg cccgtaattc ggtgagcgcc ggtgatgcca cctgagcgcc ttccagtgat
     8521 agcggttcgt cagtacccaa ccgccagctg ttgcagcgac tgccttgcgg cagcatcgcg
     8581 attttttctg gcgtcagttg cagcacttga tcggggctga cggttaatgc gcgcagaaca
     8641 tcgctcacta aaggatcggt cagggcggga agatcgtttg ccaccatcac cagacggacg
     8701 tgtgccggga tcgcaatggc aatctccccc tgcaacgcgc cagggcgacg cagcgaccac
     8761 tgggtaatgc ccagttgctg taactgccag tctcgtcggg atgtcatagc gaatcgctcc
     8821 tgttgtcagg ggcgcaaata tagcaaattc gtcgataccg cgccaacata tggctataat
     8881 cgccgccagt atcaattgag gagcattcca tgtctgcatt taccccggca agtgaagtct
     8941 tgctgcgtca cagtgatgat ttcgaacaaa gccgtattct gtttgccgga gacttacagg
     9001 atgacctgcc cgcgcgttta gataccgcgg ccagccgtgc tcatacccag caattccacc
     9061 actggcaggt attaagccgc cagatggggg ataacgcccg tttcagtctg gtcgccacgg
     9121 tggatgacgt cgcagattgc gatacgctga tttactactg gccgaagaac aaaccggaag
     9181 cccagttcca gttgatgaat ttactttctc tgctgccagt ggggacagat atttttgtcg
     9241 ttggcgagaa ccgcagcggc gtgcgcagcg ccgagcagat gctggcagat tatgcgccgt
     9301 tgaataaagt cgacagcgct cgtcgctgtg gcctctattt tggtcgtctg gaaaaacagc
     9361 cggtatttga tgccgataaa ttctggggcg aatacagcgt cgatggcctg acagtcaaaa
     9421 cgctgcctgg cgtgtttagc cgcgacggtc tggatgtcgg tagccagttg ctgctctcga
     9481 cgttaactcc gcacacgaaa ggtaaagtgc tggatgtcgg ctgtggcgcg ggggtgcttt
     9541 cagttgcctt tgcgcgccat tcgccgaaaa ttcgtctcac cttgtgcgat gtctctgcgc
     9601 cagcggtaga agccagccgc gcaacacttg cggccaactg tgttgaaggt gaagtctttg
     9661 ccagcaacgt cttttccgag gtgaaaggtc gttttgatat gatcatctcc aacccgccgt
     9721 tccacgatgg gatgcaaacc agcctggatg cggcgcaaac gctgattcgc ggcgcggtgc
     9781 gtcatcttaa tagtggcggc gagctgcgaa ttgtagcgaa cgccttcctg ccttatccgg
     9841 acgtgctgga tgaaacattt ggcttccatg aagtgattgc gcaaaccggg cgtttcaagg
     9901 tatatcgcgc cattatgacc cgccaggcga aaaaaggtta agtatcccgc cgcaagcctt
     9961 gccggatgcg gcgtaaacgc cttatccgac ctacaaaacc tgcacgttag cccttcgtag
    10021 gccagataag acgcgccagc gtcgcatctg gcatctgccc ccgcatgcct accaaacctg
    10081 cacgctagtt tcctgatgga catttttcca gcaattacac ctccggcgat aattaactat
    10141 tgacgaaaag ctgaaaacca ctagaatgcg cctccgtggt agcaattctt tttaagaatt
    10201 gatggtatgc gaaggtggcg gaattggtag acgcgctagc ttcaggtgtt agtgtcctta
    10261 cggacgtggg ggttcaagtc ccccccctcg caccaattat ctttacttcc tttcttgttt
    10321 cttccttgat ttttcatccc tgattcaaat tcattatcat cagcatgatg gcagcaataa
    10381 gcgccaggca cgatggtagc aaaacgtaat gccgtaactg tttgtgatac agcatcaccg
    10441 gcgtcaatac taatcccatc agaatgacaa tccgccactg atccggcgat aaatccgcat
    10501 acatcagccc tgcaatcagc aatcccggca gcaacactcc ccagccactg cccagcgtac
    10561 gttgcaacat ggtttcatct ctttcattga taatgataac taatatcata tgataatttt
    10621 tatcatttgc aagccagaga aatcccttgc tatcgggtaa acctatcgct aggattagca
    10681 atcattatca tttagattac tatcccgatt atggcctatc gttccgcacc gctctatgaa
    10741 gatatcatct ggcgaacgca tctccagccg caggatgctg ggcttgcaca agccgtacgt
    10801 gcgacgatcg ccaaacatcg tgaacatttg ctcgagttta tccgcctgga tgaacccgct
    10861 ccgcttaacg ccatgacgct ggcgcaatgg tcatcaccga atgcgctaag ttctctactc
    10921 gcggtctatt ccgatcatat ctatcgcaac caaccaacga tgatccgcga gaacaaaccg
    10981 ctgatctcac tatgggcaca atggtatatc ggcctgatgg tgccaccatt aatgctggcg
    11041 ctactgacgc aggaaaaggc attagatgtg tcgccggaac atttccatgc tgagtttcac
    11101 gaaaccggac gcgtcgcctg tttctgggtc gatgtgtgtg aagataaaaa cgcaacacca
    11161 cattcgccgc agcagcgaat ggaaacgtta atcagccagg ctctggttcc ggttgtgcag
    11221 gcactagaag cgaccggaga gatcaacggc aaacttatct ggagtaatac cggttatttg
    11281 atcaactggt atctcactga gatgaagcaa ctgctcggcg aggcgacggt tgaatcgctg
    11341 cgccatgccc tcttttttga gaaaacgctc acgaacggtg aagataatcc actctggcgt
    11401 accgtcgtgc tgcgcgatgg cctgctggtg cgccgaacct gctgccagcg ttatcgctta
    11461 ccggatgtgc agcaatgtgg cgattgtacg ctgaaatgaa agcactgccg gggaagtaaa
    11521 cccggcatca tgcggattaa tagggatgca acacattact tgcttcgcaa tgccgcatac
    11581 ataacagaat atctgcggcc tccaacaccc ctgcatcaga actgacgccc agtttcgaca
    11641 tcacattaaa tttatgtgca cggatcgttt tgatattgcg tttaagctgc tcggcaattt
    11701 gtgtcattga gtagccacgc gacataaagc gcaatatttc tctctccgtc gggcttagcg
    11761 tccggctttg gttaatgtac cactgattgt tcagtcggtc ggtcgcctga cgtacaccat
    11821 ttaatgacaa aaagagttcc tgatgaaaaa tctccagcgt tgacgcttta cttaatacac
    11881 cgtccagcgg tgatggcgac aatgaaccaa tcagccgagc ttcaatatca tcatccgcaa
    11941 taactaaacg ccgggtacgc ggaaacttaa tcgccagttc agtcaggcaa gataatccct
    12001 ctctgcgctc acttctcatg gcagaaaaag aaaaatgacc gccgcaaatg agatccgcga
    12061 catggcatcc tgaaatgact cctgactggt ataggtatgg agcttataag ggttacccgc
    12121 aaactttctg aataaactct caataccgat actgctcatg acgcattttt ctatgagtgc
    12181 gacatttctc ttcttaattc ggctgtgttc cattctgcgg catctccata aacagaaggc
    12241 aagttaagtt ctcttgcacc ctgcgcgcta tttatccatg catacatttc agcgttgctg
    12301 tgaattgcca gtcgacgcat cgcactgttt ttttgggcgc tgatagtttt attacttttc
    12361 ttaagcaacg aaactatctg gttgatgccc catcctttac ctaatagccg cagtaccttg
    12421 cgctctgaaa gagtgagaat gacggagcga tagctaaaat catgagtttc attcattgcc
    12481 ggagaggtca gcatggcgct gatacgctct gcgtgcgtat tgccggaacg tacggtcttg
    12541 accagattct caatgggttc aacatcagac aataacgtcg ccgtagggca catgagcaat
    12601 tctactgcct gggaatacca ggagcgtgac accatgaaaa cccagtgaat ttcccgatat
    12661 tgtgagatta aagaataata atgttcacaa acagaacgtg gatcttcact ttcaccggct
    12721 aaatcagcaa tgactaatcc ggaacgacgt aattgtaata aggtaaagtc ctccgcagag
    12781 gcgctggata ttatttcata ttcagggaag tgagtcctca tgacctcttt taaccctgct
    12841 tgcataacag gtattttact gataacaatt ccatttttgc agcatcctgg caacatattt
    12901 cctccacatc cactgtctaa aaatcatgat ggtttattca ggatattcat tccttaaatg
    12961 tttccagcaa attaatagat taagcgaata aattatcctt agcattcact ttcatatcag
    13021 catactcctg ctgcacaaca actccactgc atgtgtcaga aggttagcat caaatgataa
    13081 cctggtgaaa tagcctggag acatttaaaa tatctacatt aagttgttag gaatacttaa
    13141 tgaaaaacag aaatcacatt catttaattt ataattaaat gatatccctt cccgcagaat
    13201 gaaaagtcac acaaccaata tttattcaat gaaatctgat ggttttaact tgttaacatt
    13261 aaaaaacgtg cccggttatc tattatcctg attcgtaaag gccaaacaat aaattcatta
    13321 tagtgataat attcatgtga tagtttgttt gcggcgagag ataattcgct ttttatcacc
    13381 gagcttgtcc cgataaccag gattaatcag cgcaattttg atcttaacct accgcaaaac
    13441 aaccgtgctt catgatacaa atggattctc attatctgaa caatacgcag cacgtctatg
    13501 acaaagggcg agttatgcaa actgagcaac agcgagccgt aacacggctt tgtatccagt
    13561 gtggattatt tcttttgcaa catggtgcgg aaagcgcgtt ggttgatgag ctttcctcac
    13621 gactgggtcg ggcgttgggg atggacagcg tcgaaagttc tatctcttcg aacgccatag
    13681 tgctgacaac cattaaagat gggcaatgcc tgacatcgac acgtaaaaat cacgatcgcg
    13741 gcattaatat gcatgtggtg actgaagtcc agcacattgt gattctggcg gagcaccatc
    13801 tgctggatta caaaggcgta gagaaacgat ttagccaaat tcagccatta cgttacccaa
    13861 gatggctggt agccttaatg gttggccttt cttgcgcctg tttctgcaaa ctcaataaag
    13921 gtggctggga tggtgccatc atcaccttct ttgccagtac ggccgcgatg tatatccgcc
    13981 agctgctggc acaacgtcat cttcatccgc agatcaactt ttgcctcacc gctttcgccg
    14041 ccaccaccat ttccggattg cttttgcaac ttcccacttt cagcaataca cccactatcg
    14101 cgatggccgc cagcgttctg ctgctggtgc cgggatttcc gttgattaat gccgtcgccg
    14161 atatgtttaa aggccacatc aataccggac tggcacgctg ggcgatcgcc agtctgctga
    14221 cactggctac ctgcgtcggc gtggtgatgg cactgacgat ttgggggcta cgcggatggg
    14281 tgtgatcgag tttctgtttg cattggcgca ggatatgatc ctcgccgcca ttccagcggt
    14341 cggctttgcg atggtgttca acgttcccgt tcgggcatta cgctggtgtg cgctgcttgg
    14401 cgcgattggt catggttcac gaatgatctt aatgaccaga gggctgaata ttgagtggtc
    14461 aacctttatg gcctccatgc tggtcggcac cattggtatt caatggtcgc gctggtatct
    14521 ggcgcatccg aaagtgttta ccgtggcggc tgttatccct atgttcccgg gcatatcggc
    14581 ttataccgca atgatttcgg cggtaaaaat cagccagtta ggttacagcg aaccgttgat
    14641 gattaccctg ttaaccaact ttcttacagc ttcatcgatt gttggtgcgt tatccatcgg
    14701 tctttccatt cctggattat ggttgtaccg caagcgccct cgcgtataaa attgcctcgc
    14761 ttaaagcaca cggatgagag acagcctcct ctcctccgtg tgttactata aaagttatct
    14821 cccttctcgt tcatcgttcc atatttgaga aacagtatgt cttccagagt tttgaccccg
    14881 gacgtcgttg gtattgacgc cctggtacac gatcaccaaa ccgttctggc aaaagctgaa
    14941 ggcggtgtgg ttgccgtatt tgctaacaat gccccggcgt tttatgccgt cacgcctgca
    15001 cgcctggctg aactgctggc gctggaagaa aagctggcgc gtccgggaag cgatgtcgct
    15061 ctggacgatc aactctatca ggaaccgcaa gccgctcccg ttgctgtacc catggggaaa
    15121 ttcgccatgt atccggactg gcaacccgat gccgatttta tccgcctggc ggcgctatgg
    15181 ggtgtggcac taagagagcc ggtgaccacc gaagaactgg cctcattcat tgcctactgg
    15241 caggcggaag gtaaagtctt tcaccatgtg cagtggcaac aaaaactggc gcgcagcctg
    15301 caaatcggtc gtgccagcaa cggcggactg ccgaaacgag atgtgaatac ggtcagcgaa
    15361 cctgacagtc aaattccacc aggattcaga gggtaacgat gaaaaacgtt ggcgacctga
    15421 tgcaacgcct gcaaaaaatg atgcctgccc atatcaaacc tgcattcaaa acgggtgaag
    15481 aactactggc ctggcagaaa gaacaaggag cgatccgctc cgccgctctc gaacgtgaaa
    15541 atcgggcgat gaaaatgcag cgcaccttta accgctccgg tattcggcca ctgcatcaga
    15601 actgctcctt tgagaactat cgcgttgagt gtgaagggca gatgaatgcg ttaagcaaag
    15661 cgcgccagta cgtcgaagag tttgacggca acatcgccag cttcatcttt tctggtaagc
    15721 caggaaccgg caaaaaccat ctggcggcgg caatctgcaa cgagctgctg ctacgcggta
    15781 aatccgtatt gatcatcacc gtggccgata ttatgtcggc gatgaaagat accttcagga
    15841 atagcggtac cagtgaagaa caactgctta acgatctgag caacgttgat ctgctggtga
    15901 tcgatgagat cggcgtgcag accgaatcga aatacgaaaa agtgatcatc aaccagatcg
    15961 tcgatcgccg ctcttcttcc aaacgcccaa ccgggatgct gaccaacagt aatatggaag
    16021 agatgaccaa gttgctgggc gaacgcgtga tggaccgtat gcgcctgggt aacagtttgt
    16081 gggtgatctt caactgggat agctaccgta gccgggtaac aggtaaagag tattaatccc
    16141 cgtttctggc ggtatactgc gacacaattc caccttgagt aatgatgatg gaaactgtta
    16201 aacatcttct gtgctgtgcc attgccgcca gcgcattaat ttccaccggg gtgcatgctg
    16261 cgtcctggaa agatgcgctc tccagcgccg ccagcgaact tggcaaccaa aacagcacga
    16321 cacaggaagg cggttggtcg ctcgcgtcat taactaactt gcttagcagc gggaaccagg
    16381 ccttaagcgc agataacatg aacaacgccg caggcattct gcaatactgc gcgaagcaaa
    16441 agctggcgtc ggtaaccgat gccgaaaaca tcaagaacca ggtgctggaa aagctgggcc
    16501 tgaacagtga agagcaaaaa gaagacacca actatctgga tggcattcag ggtttgctga
    16561 aaaccaaaga tggtcagcaa ctcaatctgg ataacatcgg aacgactccg ctggcagaaa
    16621 aggtgaaaac caaagcctgc gatctggtgt taaagcaggg gctgaacttc atttcctgaa
    16681 ccgcgaaacg ccgcgtttta gctgttaccc ggctgacgcg gctctctcct gcccttttcc
    16741 ctgcttctgg acggttttcc ccctgttaca cgggaagcga ccgcgatcaa aaaagtctgt
    16801 ttataaacta attcctaaca acatcgcatt tttttgacac tagaattgca gcaatatggc
    16861 gacgccatta cattgttctt ctcaaaaagc ttaaacgttg gctaaacggc ccggtttagt
    16921 gaggatcgcc cgttgtcaga actactctct ttcgcccttt ttctcgcctc tgtgctgatt
    16981 tacgcatgga aagcgggacg taacacctgg tggtttgcag ccacattaac ggtgctgggg
    17041 ctatttgtcg ttttaaatat caccctgttt gccagcgact attttactgg cgatggtatt
    17101 aacgacgcgg ttctctatac cttaaccaac agcctgaccg gtgctggcgt cagcaaatac
    17161 atcctgccgg gtatcggcat tgtgctgggg ctgacagcgg tgttcggtgc gctgggctgg
    17221 atcctgcgcc atcgtcgcca tcatccgcac cattttggtt acagcctgct ggcgctctta
    17281 ctggcgctgg gttcggtgga cgccagcccg gcatttcgtc agataacgga actggtgaaa
    17341 tcccagtcac gcgatggcga cccggacttt gcggcttatt ataaagagcc gtcgaaaact
    17401 atccctgacc cgaaactcaa cctggtttat atctacggcg aaagtctcga gcggacctat
    17461 tttgataacg aagctttccc ggatctcacg cctgaactgg gcgcgttgaa aaatgaaggc
    17521 ctggatttca gccacacgca gcagctgcca ggaacggatt acacgattgc gggcatggtg
    17581 gcttctcagt gcggcatacc gctgtttgcc ccctttgaag gcaacgcctc cgcctctgtc
    17641 tccagcttct tcccgcagaa catctgtctg ggcgatatcc tgaaaaactc gggttatcag
    17701 aactatttcg tgcagggcgc gaatctgcgt tttgccggta aagatgtgtt cctgaagtcg
    17761 catggcttcg accacttata cggctcagaa gagctgaaaa gcgtggtggc cgacccgcac
    17821 tatcgcaacg actggggatt ctacgacgat accgttctcg atgaagcgtg gaaaaagttt
    17881 gaagagcttt cccgctcagg tcagcgattc tcactgttta ccctgacagt cgatacccat
    17941 cacccggatg gttttatctc gcgtacctgt aaccgcaaaa aatatgattt tgacggtaag
    18001 ccgaaccagt cattcagcgc ggtaagttgc agccaggaga atatcgcggc gtttatcaac
    18061 aaaatcaaag cgtcaccgtg gtttaaagat accgtcatcg tcgtctcttc tgaccattta
    18121 gcgatgaaca acacggcgtg gaaatacctc aataagcagg atcgcaataa cctgtttttt
    18181 gtcattcgtg gcgacaagcc gcagcaagag acgctggcag tgaagcgtaa cacgatggat
    18241 aacggcgcga cggtgctgga cattctcggt ggcgataact atctcggact tggtcgtagc
    18301 agtttatccg ggcagtcgat gtcggaaatc ttcctcaata tcaaagagaa aacattggcg
    18361 tggaagccgg atatcatccg cctgtggaaa ttccctaaag agatgaaaga gttcaccatc
    18421 gaccagcaga aaaacatgat tgccttctcg ggtagccatt tccgtttgcc gctgctgttg
    18481 cgggtttcag acaaacgcgt ggaaccgctg ccggaaagcg aatactcagc accgctgcgt
    18541 ttccagctgg ccgatttcgc tccacgcgac aatttcgtct gggttgaccg ttgctataag
    18601 atggcacaac tctgggctcc ggaactggca ctctccaccg actggtgtgt ctcgcaaggg
    18661 cagcttggcg gtcagcaaat tgttcaacat gttgacaaaa caacatggca gggcaaaacg
    18721 gcatttaaag atacggtcat cgacatggcg cgttataaag gcaatgtcga tacgctgaaa
    18781 attgttgata acgatattcg ctacaaagcc gacagtttca tcttcaacgt cgccggtgcg
    18841 ccagaagagg tgaaacagtt tagtgggatt tcccgtccag agtcgtgggg ccgctggtcc
    18901 aacgcgcagc tgggcgatga agtaaaaatc gagtacaagc atccgctgcc aaagaaattt
    18961 gacctggtga ttaccgccaa agcatacggc aataacgcca gccgtcctat tccggtacgc
    19021 gtaggcaatg aagaacaaac ccttgtgctg ggcaatgaag tgaccaccac cacactgcat
    19081 ttcgataacc caaccgatgc cgacacactg gtaattgtgc cgccggaacc tgtctcaacc
    19141 aacgaaggga atatcctcgg acactcgcca cgtaagctcg ggatcggcat ggtggaaatt
    19201 aaagtggtgg aacgtgaagg ataattcctg actgatgtgt aggtcgcata aggtgcgacc
    19261 tacacacctt ctgcggccta caaaacatca ttgagaaaca gaaaaaaaca gcgcccaaca
    19321 cttccccgga cgctgcatgg caactttaat aatctcgcct tatcagaaag taattacacc
    19381 cttaatcaac tcacgattgt taatcacatc gcgctcgtag gtttctgcca gcgtggcgaa
    19441 cggatagcga tgggttaaca tcatgtcagc agtgattttt ccttccgcca tcagtcgacc
    19501 gactttagca aaatcttccg gcgtggcgtt gcggctaccc atcatcgtcg tttctttttt
    19561 atggaattcc ggatcggaga actgcaactc acctttaaac aggccgacaa ataccaccgt
    19621 gccgccgtga cgaatcaaat tcacggtgtt attcatcgca tgttgattac ctgtcgcgtc
    19681 gatcactttc tgcgccagcg aaccaccaaa ctgcgcccgt agctgcgcgt caaaatcttc
    19741 ggctgacggg tccagtaaag gtaattccag acgcgttgcc acatgttcac ggcgcgccgg
    19801 actggtatcc gccaccacca cctgtgcgcc atcggcttta gcgattgccg ccgcgcccag
    19861 accgattggc cccgccccga ccaccagcac ctgctcgccg ggagcaatgg ctgcgcgacg
    19921 caccgcatga gcgctaatag cgaaaggttc aatcaatgct gccgcctgcg ggtcaatacc
    19981 gtctgcgggc aaaatgttcg ccaccggcac gctcaaatac tcactaaaac cgccatcctg
    20041 atgcacgcca atgactgaaa ttttttcaca gcaattggta cgcccgcttt tacacgccgg
    20101 gcattgctga caggcaacat aagggatcac agcaacttgc agaccatttt taagatcagc
    20161 aatattttta cccagcctaa caatctcccc acatatttca tggcctaaaa cacgtggata
    20221 actaaaaaat ggttgatttc caccccaggc atgaatatcg gtaccgcaaa tcccgacaga
    20281 cttaattttt attaatgctt cattgtcacc cggaataggt atctcgcgtt gtttccagac
    20341 taattctttc ggctgctggc aaattaaaac attcatcgta gacatgattc ggctctccat
    20401 tttttgttgc cttagatatc aatgaaaatg aaaaatggca gcggaacgga aaatctgttt
    20461 tgtgaaaaca cacgcataaa aatgttttaa atcgggtttt aatggactca caaggaggaa
    20521 aatatgagtc gttcacaaaa tttacgccac aatgtgatta accaggtcat tgatgatatg
    20581 gcgcggggtc atatcccttc cccacttcca tcgcaaagtg cgctggcgga aatgtacaac
    20641 atcagccgca ccacagtgcg tcatattctc agccatttac gcgaatgcgg cgtcttgacg
    20701 caggttggaa acgactatgt tattgcgcgt aaacctgacc acgatgacgg tttcgcctgt
    20761 actaccgctt caatgagtga acaaaacaaa gtgtttgagc aggctttttt cacgctgata
    20821 aaccagcgcc agttacgccc aggggaaact ttttctgaac tgcaactggc tcgggccgcg
    20881 ggtgtcagcc cggtggttgt aagagaatat cttttaaaat tcggccgtta taatcttatt
    20941 caaagcgaaa agcgcggcca gtggagcatg aagcaattcg atcaatccta cgccgagcag
    21001 ctgtttgagc tacgcgaaat gctcgaaaca cattcacttc agcactttct caacctgcct
    21061 gatcatgatc cccgctggct gcaggcgaaa accatgctcg aacgtcatcg gctactgcgt
    21121 gacaacatcg gcaatagctt tcgcatgttc tcgcaacttg acagagattt tcactcctta
    21181 ctgctttcag ctgccgacaa tatctttttt gatcaatcgc ttgagatcat ctcagtcatc
    21241 tttcattttc actatcaatg ggacgaaagc gatcttaaac agcgcaatat cattgccgtc
    21301 gatgagcata tgaccattct cagcgctctg atttgccgca gtgacctcga tgccaccctt
    21361 gcgctccgta accatttgaa ttcagctaaa caatcaatga ttcgatccat taatgagaac
    21421 acgcgttatg ctcattaagt acggattaaa aacagcaaac tattagcggg tgcacggttt
    21481 tatcaaaaga taaataccgg gaaattcccg cttacctatg ctcacaatca gtcgactaaa
    21541 aatgtttttg tgagcggtag taaagtccta aaactttaac ctgccgttaa tgtcaacctt
    21601 gcgtaatgca caattacgta aacaattaaa attataaata caggagtccg gcgtggaaaa
    21661 agaaaatatc accatcgatc cgcgttcttc atttactcca tcttcgtcgg cagatattcc
    21721 cgtgccacca gatggattag ttcaacgcag tacccgaatt aaacgcattc aaaccaccgc
    21781 catgttgtta ttattttttg cggcggtaat caattatctc gaccgcagtt cgctgtcggt
    21841 agcaaattta acgattcgtg aagaattggg attaagtgcc actgaaatcg gcgctttgct
    21901 ctccgtgttt tcactcgctt acgggattgc gcaacttcct tgcggcccac tattggatcg
    21961 taaaggccca cgcctgatgc tgggactggg gatgttcttc tggtcactgt tccaggcaat
    22021 gtctggcatg gtgcacaact ttacgcagtt cgtgttggtg cgtatcggta tggggattgg
    22081 tgaagcgccg atgaacccat gcggtgtaaa agtcattaac ga
//
LOCUS       DAZPEB010000051        21506 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZPEB010000051 DAZPEB010000000
VERSION     DAZPEB010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21506
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.052"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..95
                     /locus_tag="ACHURJ_001704"
     CDS             <1..95
                     /locus_tag="ACHURJ_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR1280688.1"
                     /translation="LNAVLDYIDAVAATDTSTAPDVIWPELPEA"
     gene            complement(67..504)
                     /locus_tag="ACHURJ_001705"
     CDS             complement(67..504)
                     /locus_tag="ACHURJ_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR1280689.1"
                     /translation="MFYSPSLNIFVNPALKDDYINANSWPDDALAVSDDVYNEFAINT
                     PPDDKIRVAGKNGLPTWALIPPPSYEELIQQAESERQLLLNQANEYMNSKQWPGKAAI
                     GRLKGEELAQYNLWLDYLDALELIDTSSAPDIEWPTPPAVQAR"
     gene            complement(506..892)
                     /locus_tag="ACHURJ_001706"
     CDS             complement(506..892)
                     /locus_tag="ACHURJ_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1280690.1"
                     /translation="MPVSHYLCIFLNVGLGELSLAGTASGVIGLNGYVTIPLIISGSR
                     RTLIIQWGQARFGGSGGEDAGYLNDFPFAFPSACYGMIVSHVGHTPSGAGILSASAIT
                     SNQFRGFSSIATAANAVLGRYIAIGV"
     gene            923..1489
                     /locus_tag="ACHURJ_001707"
     CDS             923..1489
                     /locus_tag="ACHURJ_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016529756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR1280691.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALNCAGCERIFEDKISGTKPDRPG
                     LKKLLRTLSAGDTLVVWKLDRLGRSMRHLVTLIEELRQRGVNFRSLTDSIDTSTPMGR
                     FFFHVMGALAEMERELIVERTRAGLAAARAKGRVGGRRPKLTTEQWAQIGRLLEAGES
                     RQRIALIFDVGVSTIYRKFPANESNESP"
     gene            1632..2804
                     /locus_tag="ACHURJ_001708"
     CDS             1632..2804
                     /locus_tag="ACHURJ_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HFR1280692.1"
                     /translation="MAQDYHHGVRVVEVNEGTRSITTVSTAIVGMVCTGDDADAKMFP
                     LNKPVLITDVLTASGKAGESGTLARSLDAIADQAKPVTVVVRVPQGETEEETTTNIIG
                     AVTAEGKKTGMKALLSAQSQLGVKPRILGVPGHDTKAVATELLSVAQSLRGFAYLSAY
                     GCKTVQEAITYRENFSQREGMLIWPDFTGWDTVLNAEATAYATARALGLRAKIDEQTG
                     WHKSLSNVGVNGVTGISADVFWDLQDPATDAGLLNQNDITTLIRKDGFRFWGSRCLSD
                     DPLFAFENYTRTAQVLMDTMAEAHMWAVDKPLNPSLARDIIEGIRAKMRSLVSQGYLI
                     GGDCWLDESVNDKDTLKAGKLTIDYDYTPVPPLENLMLRQRITDQYLVNFSSQVSA"
     gene            2814..3329
                     /locus_tag="ACHURJ_001709"
     CDS             2814..3329
                     /locus_tag="ACHURJ_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFR1280693.1"
                     /translation="MALPRKLKHLNLFNDGNNWQGIVESLTLPKFTRKYEKYRGGGMP
                     GAVDVDLGLDDSALDTEFSIGGTELLLFKQMGKATVDGIQLRFTGSIQRDDTGEVQAV
                     ELVVRGRHKEVDSGEWKTGESNTTKVTSTNSYAKLTINGEVLYEVDLINMVEIVDGVD
                     LMEAHRNALGL"
     gene            3384..3686
                     /locus_tag="ACHURJ_001710"
     CDS             3384..3686
                     /locus_tag="ACHURJ_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFR1280694.1"
                     /translation="MSDKQTEKTIQLDTPIMRGKTEITEIVLRKPQSGALRGTRLQAI
                     MDMDVNAMMTVIPRISSPALTAQEIAEMDPADLTAMSVEVVTFLLKKSVLAGLPTA"
     gene            3701..3820
                     /locus_tag="ACHURJ_001711"
     CDS             3701..3820
                     /locus_tag="ACHURJ_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFR1280695.1"
                     /translation="MADIATIFHWPPSVTDVMPLTEVLEWRDKAIQRSGASHE"
     gene            3813..6890
                     /locus_tag="ACHURJ_001712"
     CDS             3813..6890
                     /locus_tag="ACHURJ_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR1280696.1"
                     /translation="MSDNNLRLQVILNAVDKLTRPFRSAQASSRELAAAVKKSRDAIK
                     QLDQAGSSLDSFRKLQAENQKLGDRLNYARQRANLLSQELGAMGPPSQRQVVALGRQR
                     LAVQRLEERQKKLQQQTALVRAELYRAGISANDGASATARITRETMRYNRQLSEQEAR
                     LRCVGEQQRKMHAARGAYARRLEVRDRIAGAGATTTAAGLAMGAPVMAAVKSYTSMED
                     AMKGVAKQVNGLRDDNGNRTARFYEMQGAIKAASEQLPMENGAVDFAALVEGGARMNV
                     ANPDDSWEDQKRDLLAFASTAAKAATAFELPADELSESLGKIAQLYKIPTRNIEQLGD
                     ALNYLDDNAMSKGADIIDVMQRLGGVADRLDYRKAAALGSTFLTLGAAPEVAASAANA
                     MVRELSIATMQSKSFFEGMNLLKLNPEVIEKQMTKDAMGTIQRVLEKVNALPQDKRLS
                     AMTMLFGKEFGDDAAKLANNLPELQRQLKLTAGNDALGSMQKESDINKDSLSAQWLLV
                     KTGAQNTFSSLGETLRQPLMDILYTVKSVTGALRRWVEANPELTGTLMKVAAVVAAVT
                     VGLGTLAVALAAVLGPLAVIRLGFSVLGIKTLPSVTATVTRTSNALSWLAGAPLALLR
                     RGLASSGNAAGLLTAPLSSLRRTASLTGNVLKTVAGAPVALLRSGLSGLRAVAVMFMN
                     PLAVLRGGLAATGTVLRMLASGPLAMLRVALYAVSGLLGALLSLIGLVVTALAGVALV
                     VWKYWQPITAFLGGVVEGFKAAAGPVSAAFEPLKPVFQWIGDKVQALWGWFTDLLTPV
                     KSTSAELQSAAAMGRRFGEALAEGLNMVMHPLDSLKSGVSWLLEKLGIVSKEAAKAKL
                     PESVTRQQPATVNADGKVMMPSGGFPSWGYGFAGMYDSGGYIPRGQFGIVGENGPEIV
                     NGPANVTSRRNTAALAAVVAGMMGVAATPAELPPLHPLALPAKGGEAMVSRAATVPPV
                     QRIEAPMQIIIQTQPGQSAQDIAREVARQLDERERRLKAKARSNYSDQGGYDA"
     gene            6887..7372
                     /locus_tag="ACHURJ_001713"
     CDS             6887..7372
                     /locus_tag="ACHURJ_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1280697.1"
                     /translation="MMMVLGLYVFMLRTVPYQELQYQRSWRHAANSRVNRRPSTQFLG
                     PDNDMLTLSGVLMPEVTGGRLSLLALEQMAEQGKAWPLIEGSGTIYGMYVIEGLNQTK
                     TEFFRDGMPRRIEFTLSLKRVDESLSDMFGDLSAQLNNLQGTATSALSDISKTVGGLL
                     S"
     gene            7369..8469
                     /locus_tag="ACHURJ_001714"
     CDS             7369..8469
                     /locus_tag="ACHURJ_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HFR1280698.1"
                     /translation="MNFSSELLNKGNKTPAFSISIEGRDITTVLDNRLMGLTLTDNRG
                     FEADQLDLELDDADGKIVLPRRGAVITLALGWKGQPLFPKGAFTVDEIEHTGAPDRLT
                     IRARSADFRETLNTRREKSWHKTTVGEVVKEIAARHKLKMALGEDLSDKLVEHIDQTN
                     ESDGSFLMRLARQYGAIASVKNGNLLFIRQGQGKSASGKPLPVITITRKDGDSHRFTL
                     ADRGAYTGVIASWLHTREPAKKESTTVKRKRRTKKQKKEPEAKQGDYLVGTDENVLVL
                     NRTYANRSNAERAAKMQWERLQRGVASFSLQLAEGRADLYTEMPVKVSGFKQPLDDAE
                     WTITTLTHTVSPDNGFTTSLELEVRIDDFEME"
     gene            8560..8778
                     /locus_tag="ACHURJ_001715"
     CDS             8560..8778
                     /locus_tag="ACHURJ_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001504085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFR1280699.1"
                     /translation="MMICPLCGSAAHTRSSFQVSSLTKERYNQCQNINCSHTFVTHET
                     FVRSIATPKESNPVQPHPMKSGQVALSL"
     gene            complement(9014..10699)
                     /locus_tag="ACHURJ_001716"
     CDS             complement(9014..10699)
                     /locus_tag="ACHURJ_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFR1280700.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTAHSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            10969..11346
                     /locus_tag="ACHURJ_001717"
     CDS             10969..11346
                     /locus_tag="ACHURJ_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFR1280701.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(11376..11633)
                     /gene="grxA"
                     /locus_tag="ACHURJ_001718"
     CDS             complement(11376..11633)
                     /gene="grxA"
                     /locus_tag="ACHURJ_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFR1280702.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            11793..12080
                     /locus_tag="ACHURJ_001719"
     CDS             11793..12080
                     /locus_tag="ACHURJ_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFR1280703.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            12064..12786
                     /gene="nfsA"
                     /locus_tag="ACHURJ_001720"
     CDS             12064..12786
                     /gene="nfsA"
                     /locus_tag="ACHURJ_001720"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFR1280704.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSDRATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            12847..13749
                     /gene="rimK"
                     /locus_tag="ACHURJ_001721"
     CDS             12847..13749
                     /gene="rimK"
                     /locus_tag="ACHURJ_001721"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFR1280705.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIVHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERYATTEYCLKTGG"
     gene            13837..14313
                     /locus_tag="ACHURJ_001722"
     CDS             13837..14313
                     /locus_tag="ACHURJ_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFR1280706.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            14664..15776
                     /gene="potF"
                     /locus_tag="ACHURJ_001723"
     CDS             14664..15776
                     /gene="potF"
                     /locus_tag="ACHURJ_001723"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFR1280707.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            15871..17004
                     /gene="potG"
                     /locus_tag="ACHURJ_001724"
     CDS             15871..17004
                     /gene="potG"
                     /locus_tag="ACHURJ_001724"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFR1280708.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTQLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVHEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERHEDGLVLYSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            17014..17967
                     /gene="potH"
                     /locus_tag="ACHURJ_001725"
     CDS             17014..17967
                     /gene="potH"
                     /locus_tag="ACHURJ_001725"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFR1280709.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVAAAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            17964..18809
                     /gene="potI"
                     /locus_tag="ACHURJ_001726"
     CDS             17964..18809
                     /gene="potI"
                     /locus_tag="ACHURJ_001726"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFR1280710.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            18869..19357
                     /locus_tag="ACHURJ_001727"
     CDS             18869..19357
                     /locus_tag="ACHURJ_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFR1280711.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            19398..20525
                     /gene="rlmC"
                     /locus_tag="ACHURJ_001728"
     CDS             19398..20525
                     /gene="rlmC"
                     /locus_tag="ACHURJ_001728"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFR1280712.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGFRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(20700..21431)
                     /gene="artJ"
                     /locus_tag="ACHURJ_001729"
     CDS             complement(20700..21431)
                     /gene="artJ"
                     /locus_tag="ACHURJ_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFR1280713.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
BASE COUNT         5156 a         5269 c         5917 g         5164 t
ORIGIN      
        1 gacttaacgc cgtgttggat tacattgacg cggtggcggc aacagatacc agcaccgcgc
       61 cggatgtcat ctggcctgaa ctgccggagg cgtaggccat tcaatatctg gagcactgga
      121 ggtatcaatc agttccagtg cgtccagata atccagccac aaattatatt gcgccagttc
      181 ctcacctttc agacgaccaa tagcggcttt accgggccat tgtttactgt tcatgtattc
      241 gttggcctga ttaagcaata attgcctttc tgattctgcc tgttgaataa gttcttcata
      301 tgatggtgga ggtattagtg cccatgtggg taatccattt tttcctgcaa cacgaatttt
      361 gtcatctggg ggcgtattga ttgcaaattc attataaaca tcatcactga cagccagagc
      421 atcatctggc catgaatttg cattaatgta atcatcctta agcgcaggat tcacaaaaat
      481 gtttaaagat ggactataaa acatattaaa cccctatagc gatataacga cctaatacag
      541 catttgcagc agtcgctatg cttgaaaaac cgcggaactg atttgatgta attgcagaag
      601 ccgacaggat tcctgcgcct gaaggtgtat gccccacatg actaactatc atcccataac
      661 atgctgacgg aaaggcaaaa ggaaaatcat taagatatcc tgcatcttca ccaccagacc
      721 caccaaatct cgcctgcccc cactgaataa tcagtgttct ccgggaacct gaaataatta
      781 acggaatcgt tacataccca ttcaggccaa tgacacccga tgcagtgcca gccagagata
      841 attctcccaa accaacgttt aagaaaatgc agagataatg gctaactggc atcatccccg
      901 gtttttattc aggggattga tcatgcttat tggctatgta cgcgtgtcaa caaatgacca
      961 gaacaccgat ttgcaacgta atgcactgaa ctgcgcggga tgtgagcgga tttttgagga
     1021 taaaatcagt ggcactaagc ccgacagacc ggggcttaaa aaactgctca ggacactatc
     1081 ggcaggagac acgctggttg tctggaagct ggacagactg ggacgcagta tgcggcatct
     1141 tgttacgctg atagaagagt tgcgccagcg tggtgtgaat ttccgaagcc tgactgacag
     1201 tattgatacc agtaccccaa tgggccgttt cttttttcac gtcatggggg ctctggctga
     1261 aatggaacgt gaactgatag ttgaacgtac cagagcaggg ctggctgcag ctcgtgccaa
     1321 aggcagagta ggtggacgcc gtcctaagtt gaccaccgaa cagtgggcac agattggacg
     1381 tttactcgag gccggagaat caagacagcg tattgcactg atttttgatg tgggcgtttc
     1441 taccatttat agaaaatttc cggcgaatga gagcaatgaa tcgccctgag tcagtattat
     1501 tttgattttt cctgtaagcg gacaaatacc gtcattttgt gtgaataacg acacaactgc
     1561 gcttagctgt ttgtcaggca caatcacttc aacatagggc gaagcctaat ccaatcagga
     1621 ggttcgccac tatggctcag gattaccacc acggggtgcg cgttgttgaa gtcaacgaag
     1681 gcacccgatc cattaccacg gtgagcaccg ccatcgtggg tatggtctgc acgggcgatg
     1741 atgccgatgc aaaaatgttt cctcttaata aacctgtgct gatcactgat gtgctgaccg
     1801 ccagcggtaa agcgggtgag tctggcacgc tggcccgttc gctggatgcc atcgctgacc
     1861 aggcaaaacc cgtgaccgtt gttgtgcgtg tgccgcaggg tgaaacggaa gaagaaacca
     1921 caaccaatat cattggcgca gtgaccgccg agggtaaaaa aacaggtatg aaagccctgt
     1981 tatctgccca gtcacagctc ggcgttaaac cgcgcattct cggcgtgcca ggtcacgaca
     2041 cgaaggcggt agctactgag ttgctgagcg tggcgcaaag cctgcgtgga tttgcttacc
     2101 tgtcagcgta tggttgcaag acggtgcagg aagcaatcac ttaccgtgaa aacttcagtc
     2161 agcgtgaagg aatgctgatc tggcccgact ttactggctg ggacacggtg ctgaatgccg
     2221 aagcaacggc ttatgccacc gcccgtgcgc tcggtctgcg cgctaaaatt gatgagcaga
     2281 ccggatggca caaaagcctg tccaacgtgg gcgtgaacgg tgtcaccgga atttctgctg
     2341 atgtgttctg ggatctgcag gacccggcaa ccgatgcagg tctgcttaac cagaacgata
     2401 tcaccacgct tatccgcaaa gacggtttcc gcttctgggg ttcccgctgt ctgagcgatg
     2461 atccgctctt tgccttcgaa aactacaccc gcacggcgca ggtgctgatg gacacgatgg
     2521 cagaagcgca catgtgggcg gtggacaaac cgctgaaccc gtcgctggcc cgcgacatta
     2581 tcgaaggtat ccgcgccaaa atgcgtagcc tggtcagtca gggctatctc attggtggtg
     2641 attgctggct ggacgagtcg gtgaacgata aagacacgct gaaagccggg aaactcacca
     2701 tcgattacga ctacacgcca gtgccgccac ttgaaaacct gatgctgcgt cagcgcatca
     2761 ccgatcagta cctggtgaat ttctccagcc aggtcagcgc gtaaggggac aacatggctt
     2821 taccacgcaa attaaaacac ctgaacctgt ttaacgacgg gaacaactgg caggggatcg
     2881 ttgagtcgct gacgctgccg aaattcaccc gcaaatatga gaagtatcgc ggcggcggta
     2941 tgcccggagc ggtggatgtg gatctgggac tggatgacag tgcactggat acagaatttt
     3001 ccattggtgg tactgaactg ctgctgttta agcagatggg caaagccacg gtggatggca
     3061 tccagctgcg ctttaccggc tctattcagc gtgatgatac cggggaagtg caggccgtgg
     3121 agcttgtggt gcgtggacgt cacaaagaag tagattccgg cgagtggaag acgggcgaaa
     3181 gcaacactac caaagtgacc agtaccaaca gctacgcgaa gctgaccatc aatggtgagg
     3241 tgctctatga agtggacctg atcaacatgg tggaaattgt ggacggtgtg gacctgatgg
     3301 aagcgcaccg caacgccctc ggcctctgat gtatctgaac ggcgcggaat gccgcgccag
     3361 aacctaattt acaggacaac aaaatgagcg ataagcagac tgaaaagacc attcaactgg
     3421 atactcccat catgcgcggt aaaacagaaa ttaccgaaat tgtgttgcgt aaaccgcagt
     3481 ccggtgcgct gcgcggcaca cgcctgcagg ccattatgga tatggatgtg aacgcgatga
     3541 tgaccgtgat cccccgcatc tccagtccgg cactgactgc acaggaaatt gcagagatgg
     3601 acccggcaga tctcactgcc atgtcggttg aggttgtcac ttttttgttg aagaagtcgg
     3661 tgcttgccgg tttaccgaca gcctgacggt tgacgatctg gtggctgata tcgccaccat
     3721 ctttcactgg ccgccatccg ttactgacgt tatgccgctg accgaagtgc tggaatggcg
     3781 ggataaagcg attcagagaa gcggggccag ccatgagtga caataacctg cgtctgcagg
     3841 tgattcttaa tgcggttgac aaactcaccc gcccatttcg atccgcgcag gccagttcaa
     3901 gagaactggc tgctgctgtc aaaaaatccc gcgatgcaat aaagcagctt gatcaggccg
     3961 ggagcagtct ggacagcttc cgaaagctgc aggcagaaaa tcagaaatta ggcgacaggc
     4021 tgaactatgc ccgccagcgt gcaaatttgc tcagtcagga actgggagcg atggggccgc
     4081 cttcgcaacg tcaggttgtt gctctgggcc gtcaacggct ggctgttcag cgcctagaag
     4141 aacgccagaa aaagctgcag cagcagacgg cgcttgtgcg tgcagaactt tatcgtgctg
     4201 gtatttcagc taatgatggc gccagtgcga cggcccgcat tacccgtgaa acgatgcgtt
     4261 ataacaggca gctttctgag caggaagcga ggttacgatg tgtcggggag caacagcgaa
     4321 aaatgcacgc cgcccggggg gcttacgcca ggcgtcttga ggtaagggat cgtattgcag
     4381 gtgccggagc caccaccacg gctgcagggc tggcaatggg cgcaccagtg atggcggcag
     4441 taaaaagcta taccagcatg gaagatgcca tgaaaggtgt ggcaaagcag gtcaatggtc
     4501 tgcgtgacga taatggcaac cgcactgcgc gtttttacga aatgcagggt gccatcaagg
     4561 ctgccagcga acagttgccg atggaaaacg gtgctgtgga cttcgccgca ctggttgaag
     4621 gtggtgcgcg catgaacgtc gcaaaccctg acgacagctg ggaagaccag aaacgtgacc
     4681 tgctggcctt cgccagcacg gcggcaaagg cggcaacagc ctttgaactg ccagcggatg
     4741 aactgtcaga aagtctgggg aaaatcgccc agctctacaa aatccctacc cgcaatattg
     4801 agcagctcgg tgatgcgctg aactatctgg atgataacgc catgtcgaaa ggggcggaca
     4861 tcattgatgt catgcaacgc ctgggcggtg tggctgaccg tctggattat cgtaaagcgg
     4921 cggcgctggg ttccaccttc ctgacactcg gcgctgcgcc tgaggttgca gccagtgcag
     4981 caaacgcgat ggtgcgtgaa ttgtccattg ccaccatgca aagcaagagt ttctttgaag
     5041 ggatgaatct gctgaaactc aatccagaag tgattgaaaa gcagatgacg aaggatgcga
     5101 tgggaactat ccagcgggtg ctggagaagg tgaatgcgct gccacaggat aagcgcctgt
     5161 ctgccatgac catgttgttt ggtaaagagt ttggcgatga cgcagcgaaa ctggcaaaca
     5221 acctgccgga acttcagcgc cagttaaaac tgactgcggg caatgatgcg ctcggttcca
     5281 tgcagaaaga atccgacatc aacaaggatt cactttctgc tcagtggttg ctggtcaaaa
     5341 ccggagcaca gaacaccttc agcagcctgg gggaaacgct gcgccagccg ctgatggata
     5401 ttctgtacac ggtgaaaagc gtcacggggg cgttgcgtcg ctgggtggaa gctaacccgg
     5461 aactgacggg cactctgatg aaagtagccg ctgtggtagc tgccgttacc gtgggcctcg
     5521 gcaccttagc ggtggcgctg gctgcagtgc tggggccgct ggcagtcatc cgtctgggat
     5581 tctctgtgct gggtatcaaa acgttacctt ccgttacggc aacagtaacc cgaaccagca
     5641 acgcgttgtc ctggctggct ggcgcaccac tggcactgct gcgacgcggg cttgcttcat
     5701 cgggcaacgc tgcaggttta cttactgcgc cgttgtcgtc tttgcgccgc acggcatcac
     5761 tgacgggaaa tgtcctgaaa actgtagcag gtgcgccggt tgcacttttg cggtctggat
     5821 tatccggttt acgtgcggtt gctgtgatgt ttatgaatcc actggcggta ctacgcggcg
     5881 gactggctgc cacaggcacg gtgctgcgta tgctcgcatc cggtccactg gcgatgctgc
     5941 gcgttgccct gtatgccgtc tctggtctgt taggtgctct gctcagtctg ataggtcttg
     6001 tggttactgc actggcgggt gtggcgctgg ttgtctggaa atactggcag cccatcaccg
     6061 catttctcgg tggcgtggtg gaaggattca aagcggcggc aggtcctgtc agtgccgcgt
     6121 tcgaaccgct taagcctgtg ttccagtgga ttggcgacaa agtacaggcg ctgtggggct
     6181 ggtttactga tctgctgacg cccgttaagt cgacctctgc cgaactgcag agtgcagcgg
     6241 caatggggcg acgattcggg gaggctctgg cggaagggct gaatatggtc atgcatccgc
     6301 tggactccct gaaatccggc gtttcctggt tgctggagaa acttggcatt gtcagtaaag
     6361 aggccgcaaa ggcgaaactg ccggaaagcg tgacgcgtca gcaacctgcg acggtgaatg
     6421 cagacggtaa agtgatgatg ccatcgggtg gttttccgtc atggggatat ggctttgcgg
     6481 ggatgtatga cagcggcggg tatatcccgc gcgggcagtt tggcatcgtc ggtgaaaacg
     6541 ggccggaaat tgttaacggc ccggcaaatg tgaccagccg gagaaataca gctgcactgg
     6601 ctgccgttgt tgccggaatg atgggtgttg ctgctacgcc agcagagctt ccaccgttgc
     6661 accctttggc acttcccgcg aaaggcggtg aagcgatggt gagtcgtgca gccactgtgc
     6721 cgcccgttca acggattgag gcaccgatgc agatcatcat tcaaacgcag ccaggacaaa
     6781 gtgcgcagga tattgcgcgg gaggtggccc gccagcttga tgaacgtgaa cgcaggctga
     6841 aggcaaaagc caggagtaac tacagcgatc aggggggata cgacgcatga tgatggtgct
     6901 gggattgtac gtgtttatgc tgcgcaccgt gccgtatcag gagctgcagt atcaacgcag
     6961 ctggcgacat gcggcaaaca gccgggtaaa ccgacgcccg tcaacgcagt ttctgggacc
     7021 ggacaacgac atgctgacgc tttctggtgt tcttatgccg gaggtaacgg gcggcaggct
     7081 gtcgttgctg gctctggagc agatggcaga acagggaaaa gcatggcccc tgattgaagg
     7141 cagcggcacg atttacggca tgtatgtgat tgagggactg aatcagacta aaacggagtt
     7201 tttccgcgac ggtatgccgc gccggattga gttcaccctg tcgctaaaac gcgtggatga
     7261 atccctgtcc gatatgttcg gtgatctcag tgcgcaactg aataatctgc agggaacggc
     7321 aacatctgcc ttaagcgata tcagtaaaac ggtgggaggg ctgctgtcgt gaatttcagc
     7381 tctgaactgc ttaacaaagg caacaaaact ccggcattca gcatcagtat tgaaggcagg
     7441 gatatcacca ctgtgctgga caaccgcctg atggggctga cgctgacgga taaccggggc
     7501 tttgaagcgg accagcttga tctggagctg gacgacgccg atggaaaaat cgtgctgccg
     7561 cgccgtgggg ctgtcattac gctggcgctg ggctggaagg gacagccgct tttcccgaaa
     7621 ggggcattca cggtggacga gattgaacac actggcgcac cggatcgcct gactatccgg
     7681 gcgcgaagtg ctgattttcg tgaaacgctg aatacccgcc gtgaaaagtc gtggcacaag
     7741 accaccgttg gggaagtggt gaaggaaata gctgcgcggc acaaactgaa gatggcattg
     7801 ggtgaagact tgtcggataa actcgtggag catatagacc agactaatga gagtgacggc
     7861 agttttttga tgcggctggc gcgccagtac ggtgctattg cgtcggtgaa aaatggcaat
     7921 ctgttattca tccggcaggg gcagggtaaa agcgccagcg gtaaaccact accggtgatc
     7981 actatcacac gcaaggacgg cgacagtcac cgctttaccc tggcagatcg cggagcttac
     8041 acgggcgtca ttgccagctg gttgcatacc cgcgaacccg cgaagaaaga aagcaccacg
     8101 gtgaagcgta agcgcaggac taagaagcag aagaaagagc cggaagcgaa gcagggcgat
     8161 tacctggtgg gtacggatga aaacgtgctg gtacttaatc gcacttatgc caaccggagc
     8221 aacgccgaac gggcagcgaa aatgcagtgg gaacgcctgc aacgcggcgt tgcatcattc
     8281 tcgctacaac tggcggaagg gcgggcagat ctctacacag aaatgcctgt gaaggtcagt
     8341 ggctttaaac agccgctaga tgatgcggaa tggaccatta cgactctgac gcatactgtc
     8401 agcccggata acggttttac gaccagtctg gagcttgaag tgaggattga tgatttcgaa
     8461 atggaatgat tcttcgcaat ggagaacttt taagtttgca aaatggaata atgcggtatc
     8521 attattgtga atttagcaaa aatggggaga actcgaaaaa tgatgatttg cccactgtgt
     8581 ggaagtgccg cccatactcg cagcagtttt caggtatctt cattgaccaa agagcgttac
     8641 aaccagtgcc agaacattaa ctgcagccat acttttgtta cccatgaaac ttttgttcgt
     8701 tcgattgcaa cgccaaaaga gtcaaatccg gttcagccgc atccaatgaa atcaggacag
     8761 gtggcgctct ctctttgacg ctgccgccat tttgtcgcca tcgttaaaaa acagtgcttc
     8821 taacatcatg attttaaaag gcataaattt cagacaacaa aaaacccatc aaccttgaac
     8881 cgaaatggcg gggttgatgg gctccacaaa atggggacat caaagaaaag cagtggcact
     8941 aattaagact gatgccctgc ggaaaagttc tgcggttgtg caaaaaaatt tcattttcag
     9001 ggcaacttca gttttatcct aatcctggcc ataccatgac gatgattgtc cctgccagcg
     9061 tcagcaggac gttggcgatt gcgtaggtgc ccgcatagcc cagcgcaggg atgttactgt
     9121 gagctgtatc actgatgatc tccattgccg gcgcgcaggt gcgtgcgccc atcattgcgc
     9181 cgaacaacag tgcgcggttc attcgcaata cataagcacc gaacaagaaa cagataacca
     9241 cgggcacaag actgacaatt aatccggcaa tcaacatctg accgccaatc gcgcccaggc
     9301 cgttattaat accgctaccg gcgctcagac caacgcctgc cataaacacc atcaagccga
     9361 actctttcac catgcttaat gcaccttgcg gaatgtaacc gaaggtcggg tggttagcac
     9421 gcataaagcc cagcataatt ccggcgaata acaacccggc agcgttcccc atgccgaaac
     9481 tgaatgtgct gaactggaag gtgatcatcc cgatcatcag cccaataaca aagaaggcgc
     9541 aaaatgccag caggtcagtg acctggctgt gaatcgagat aaagccgatg cgatcggcga
     9601 tggtttttac gcgacgggca tcaccgctga cttgtaaaac gtcacctttg ttaagcacga
     9661 cgttgtcatc tatcggcatc tcaatctggc tacgaatgac gcggttaagg aagcaaccgt
     9721 gatcggtcaa cttcagttgt gcgagacgtt tacctacagc gttatggttt ttaacgacca
     9781 cttcttcagt gacgatacgc atgtcgagaa ggtcacgatc gaaaacttct ttaccgttac
     9841 ggaagctggg atcgagtcgg gcatgggcgt cgggatagcc taccaacgct atttcatcgc
     9901 ccatttgtag cacggcatca ccgtctggat ttgccagaat cccgttacgt cgaatacgtt
     9961 caatgtagca gccggtttgt cgataaatac ccagttcacg cagatttttg ccgtcggtcc
    10021 aggccaccag ctccgggccg acgcgatagg cgcggatcac cggtaaataa accttacggt
    10081 tggcatcagt gtccaggcca cgttcgcggg cgatttgctg ggcgctggtc tgtaagtcct
    10141 gatgctgcaa tttcggcaag taacgcgcac caacaatcaa actcaccaga ccgattaaat
    10201 aggttaaggc atacccgagg ctcagattat ccagtgccag tgagagctgc ctgctttcca
    10261 tgccggaatg acgcagtgta tcgccagcac cgaccagaac cggtgtcgac gtcatagagc
    10321 ctgctaacat accggccgtc aggccaatat cccagccaaa cagcttacct aaccctaagg
    10381 cgatcaccag cgcactgcca accatcacca gtgctaacat taggtaattt ttcccatcgc
    10441 gaaaaaaaat ggaaaaaaag ttcggtccgg cttcgacccc gacgcagaaa ataaacagca
    10501 taaagccaag attaagcgca tcggtgttaa tgctgaaatg ttgttggcct aataacagcg
    10561 atacgactaa aacgccaatg gaattaccca gttggatcga accaagtcgt aactttccga
    10621 gacatagccc aagcgcgagg accacaaata ataacagaat gtaattccca tttaacaatt
    10681 cggcgacgtt tatattcacg gaggctaact tcttgtttac tagtaagctg ttgaaagaaa
    10741 tcgtaattta cgataatgtt ttttaccaga attcagggcg cagattcatt cagcgcacct
    10801 aaacgatagt aaagtaacaa tatattttac tagtgtaatc acattaggta tcaacggcta
    10861 tatgaattgc gttggcctat attagcatgg aatgcgaagc ggctttatct tactgaacgc
    10921 cacactggcg aaaaatgtgt tcgatagacg cagtgtcagg aggaacgagt gaaacataaa
    10981 caacgttggg cgggggcaat ctgctgtttt gtcctcttca ttgtggtgtg cctttttctg
    11041 gcgacgcaca tgaaaggcgc ttttcgggct gccgggcatc ctgaaatcgg cttgctgttt
    11101 ttcattcttc ctggagcagt tgccagtttc ttttcacagc gtagagaagt cctgaaacct
    11161 ctatttggcg caatgctggc ggcaccctgt tcaatgctca ttatgcggct gtttttttca
    11221 ccgacgcgct cattctggca agagctggca tggttactaa gcgcggtgtt ctggtgtgcg
    11281 ctgggggcac tgtgtttctt atttatcagt agtttgttta aaccacagca cagaaaaaat
    11341 cagtaaagcc ctcaacgcga gggcttgtca gacgatcagg cgtccagatt ttctttcacc
    11401 catgcagcaa aatcggtata gccgccgata tgttgctgat cgacaaaaat ctgcggcacg
    11461 gtttctacgg gtttacctgc cttttgttgt agatcttctt tagtgatccc ttccgcacga
    11521 atatctacat actgatactg aaaatcatcg cgttcattgc tcaatttctc agccagatct
    11581 tttgcacgca cacagtaagg gcaacccgaa cgaccaaaaa taacggtttg cattatttct
    11641 ctcctcatag atttatgcct gtaatgatca cgctaaaatg tattcgctga aagtaggttt
    11701 aacctgttgc attaattgct aaaagctata actgttaaac acaatacagt gaaaagtttt
    11761 agactgaagg ctcactttgc agagggaagc gtatgcgcgc gatcggtaaa ttgcctaaag
    11821 gcgtgttgat actggaattt atcggaatga tgctactggc ggtggcgctg ctgtcggtaa
    11881 gcgactccct gtcgctgcct gagccatttt ctcggccaga agtgcagatt ctgatgattt
    11941 ttctcggtgt tttgctcatg cttcccgctg cggtggtggt tattcttcag gtggcaaaac
    12001 gtcttgcccc acagctgatg aaccgtccac cgcaatattc acgttcagaa agagaaaaag
    12061 ataatgacgc caaccattga acttatttgt ggccatcgct ccattcgcca tttcactgat
    12121 gaacccattt ccgaagcgca gtgtgaggcg attattaaca gcgaccgtgc gacgtccagt
    12181 tccagttttt tgcagtgtag tagcattatt cgcattaccg acaaagcatt acgtgaagaa
    12241 ctggtgacgc tgaccggcgg gcaaaaacac gtagcgcaag cggcggagtt ctgggtgttc
    12301 tgtgccgact ttaaccgcca tttacagatc tgtccggatg ctcagctcgg cctggcggaa
    12361 caactgttgc tcggtgtcgt tgatacggca atgatggcgc agaatgcatt aaccgcagcg
    12421 gaatcgctgg gattgggcgg ggtatatatc ggcggcctgc gcaataatat tgaagcggtg
    12481 acgaaactgc tgaaattacc gcagcatgtt ctgccgctgt ttgggctgtg ccttggctgg
    12541 cctgcggata atccggatct caagccgcgt ttaccggcct ccattttggt gcatgaaaac
    12601 agctatcaac cgctggataa agacgcactg gcgcagtatg acgagcaact ggcggaatat
    12661 tacctcaccc gtggcagcaa taatcgccgg gatacctgga gcgatcatat ccgccgaaca
    12721 atcattaaag aaagccgccc atttattctg gattatttgc acaaacaggg ttgggcgacg
    12781 cgctaaaacc gccacgtcga tgtatgatac gcgggctttt gaccaggtct gacagagagg
    12841 tgcagggtga aaattgccat attgtcccgg gatggaacgc tctattcgtg taagcggctg
    12901 cgtgaagccg ctatacagcg cggtcacctg gttgaaattc ttgatccgct ttcttgctac
    12961 atgaacataa atcctgcggc gtcttctatt cactacaaag gccgcaagtt accccatttt
    13021 gacgcagtga tcccgcgtat tggcaccgcc attacctttt atgggacggc ggcactgcgc
    13081 cagttcgaga tgctggggag ctatccgctc aatgagtcgg tcgccattgc ccgggcgcgt
    13141 gacaaattgc gttccatgca actgctggcg cgtcagggca tcgacctgcc tgtcacgggc
    13201 attgtgcatt cgccggatga taccagcgat ttaatcgaca tggtcggtgg tgcgccgctg
    13261 gtggtcaagt tggttgaagg cacgcaggga attggcgtcg tgctggcgga gacgcgtcag
    13321 gcggcggaaa gcgtgattga cgctttccgc ggtctgaacg cgcatattct ggtgcaggaa
    13381 tatatcaaag aggcgcaagg gtgcgatatc cgctgtctgg ttgttggcga tgaagtggtc
    13441 gctgcgattg aacggcgggc gaaagagggc gattttcgtt ccaatttgca tcgtggcggc
    13501 gcggcaagcg tcgccagtat cacaccacag gagcgtgaaa tcgcgataaa agccgcgcga
    13561 acgatggcgc tggacgttgc tggtgtggat attctgcgtg ctaatcgcgg gccgttggtg
    13621 atggaagtga atgcgtcgcc ggggctggaa ggaatagaaa aaaccactgg tatcgacatc
    13681 gcgggtaaaa tgatccgctg gatcgaacgc tacgctacga cagaatattg cctgaaaacg
    13741 ggtggttagt cgcaatcaca ttactgatca tggttttgcc tgcgcttttt gcgtaagctg
    13801 tgccggtctt tttatcgaaa gaggttgtac aaaattatga catcgctggt cgttcctggt
    13861 ctggatacgc tgcgtcaatg gctcgatgac ctggggatga gtttttttga atgtgataac
    13921 tgtcaggctc tgcatctgcc ccatatgcag aatttcgacg gtgtctttga tgccaaaatc
    13981 gatctgatcg ataacacgat cctgttttct gccatggcgg aagtccgacc ttcagccgta
    14041 ttgccgctgg cggcggattt atctgccatc aatgccagtt cgctgaccgt gaaagcattt
    14101 cttgatatgc aggatgataa tctgccaaag ctggtggttt gccagtcttt atccgttatg
    14161 cagggcgtaa cctacgagca gtttgcatgg ttcgtgcgtc agagcgaaga gcagatttcg
    14221 atggtcattc ttgaagctaa tgcccatcaa ctgctgttac cgactgacga tgaagggcaa
    14281 aacaacgtta ccgaaaacta tttcctccac tgataactcc tttcgagcac gcagtcgctg
    14341 gtgcagtgac tgcgcactgc aaaattatct gctgttttta accttttctt aaagattatt
    14401 tcacttctct tgtgccgatt tggctttatc acatagagca aatacgcata acaatttgtt
    14461 aaataccgtt ttttaatccg agctatagtc tcaaaccctg gctaaagtta ttcttgcgat
    14521 gcttttatat agcgagcagt gctggccggg agagagttat cttttcttac accgcgccga
    14581 taaaaaatat gcacgtttat tgcatatctt ttagtatgac aacttttgtt cgtttgttaa
    14641 cgaactttca gaaggaaaga gatatgaccg ccttaaataa aaaatggctt tcgggtctgg
    14701 ttgcgggtgc tctgatggcc gtctctgtcg gcacgctcgc ggctgaacaa aaaacactcc
    14761 acatttataa ctggtctgat tatatcgccc ctgacacggt ggccaatttt gaaaaagaaa
    14821 ccggtattaa agtcgtctac gatgttttcg actctaacga agtactggaa ggcaaattaa
    14881 tggcgggtag caccggcttt gacttggtgg ttccatctgc cagctttctt gaacgccagt
    14941 tgactgcggg agttttccag ccgctggaca aaagcaaatt gccggagtgg aagaatctcg
    15001 acccggagct gctgaagctg gtcgccaaac acgatcccga caataaattt gctatgccct
    15061 atatgtgggc gacgaccggg attggctata acgttgataa agttaaagct gtgctgggcg
    15121 aaaacgcgcc cgtcgatagc tgggacttga tcctcaaacc tgaaaatctg gaaaaactga
    15181 aaagctgcgg tgtctctttc ctggatgcgc cagaagaagt ttttgctacc gtgttgaatt
    15241 atctcggcaa agatcccaac agcactaaag cggatgatta caccggaccg gcaaccgatc
    15301 tgctgttaaa gctgcgcccg aacattcgtt atttccattc atctcaatac attaacgacc
    15361 tggcaaacgg cgatatttgc gtcgctatcg gctgggcagg tgatgtctgg caggcgtcaa
    15421 accgcgcgaa ggaagcgaag aatggagtga atgtctcgtt ctcgattcca aaagaagggg
    15481 cgatggcgtt ctttgatgta ttcgccatgc ctgcggatgc caaaaacaaa gacgaagcct
    15541 atcagttcct gaattacctg ctgcgcccgg atgtagtagc gcatatttcc gaccatgtgt
    15601 tctatgccaa cgccaataaa gcagccacgc cgctggtgag tgcggaagtc cgtgagaacc
    15661 cgggtattta tcctccggcg gacgttcgtg cgaagctatt cactctgaaa gtacaggatc
    15721 cgaaaatcga ccgtgtgcgc acccgcgcgt ggaccaaagt gaagagcgga aaataatccg
    15781 cagccttaga tgccgggcgg gcgcaccaca cccgccggca attcgcacca tcatggtgcg
    15841 cttgcacaca ttcaatgccg gagagcagcc gtgaatgacg ctatccctcg cccgcaggcg
    15901 aaaacccgta aggcgctgac gccgctatta gaaatccgca acctcaccaa atcctacgat
    15961 ggtcagcatg cggtcgatga tgtcagcctg accatctaca aaggtgaaat cttcgcgctg
    16021 ctgggcgctt ccggctgtgg caagtccacg cagctgcgta tgctggcagg ttttgaacaa
    16081 ccttctgccg ggcagataat gctcgatggc gtcgatttgt cacaggtgcc gccttacctg
    16141 cgccccatca atatgatgtt tcagtcttac gcgctgtttc cgcatatgac cgtggaacag
    16201 aacatcgctt ttggcctgaa acaggacaaa ctaccgaaag cggaaattgc cagtcgggtc
    16261 catgagatgc tcggcctggt gcacatgcag gagttcgcca aacgcaaacc gcatcagctt
    16321 tccggtggtc agcgacagcg tgtggcgctg gcccgaagcc ttgccaagcg cccgaaacta
    16381 ttactgctcg acgagccgat gggcgcgctg gataaaaagc tgcgtgaccg gatgcagctt
    16441 gaagtggtgg atattctgga gcgtgtcggc gtgacctgtg tgatggtcac tcacgatcag
    16501 gaagaggcga tgacgatggc gggacgtatc gccattatga atcgcgggaa atttgtccag
    16561 attggcgagc cagaagagat ctacgaacat ccaaccaccc gctacagtgc tgaatttatc
    16621 ggttcggtta atgtctttga aggtgtactc aaagagcgtc atgaggatgg gcttgtgctt
    16681 tattcgcctg ggctggtgca tccactgaaa gtcgacgcgg atgcctcggt ggtcgataac
    16741 gtgccggtac atgtggcgct gcgcccggaa aaaatcatgc tttgcgaaga gccgcccgcc
    16801 aatggttgta acttcgcggt gggggaggtg atacacattg cctatctcgg cgatctttcg
    16861 gtgtatcacg ttcgtctgaa aagtgggcag atgattagcg cccagctaca aaacgcccat
    16921 cgtcatcgta aagggttacc gacctggggc gacgaagtgc gtttgtgctg ggaagtggac
    16981 agctgtgtgg tgctgacggt ttaaggagca aagatgaata cacttgaacc tgctgcccag
    17041 tcgaaaccgc cgggcggatt taagctgtgg ttgtcgcagc tgcaaatgaa gcatgggcgc
    17101 aaactggtca ttgcgttgcc atatatctgg ttgatcttgc tgtttctgct gccatttctg
    17161 attgtcttta aaataagcct ggcggagatg gcgcgcgcta ttccacctta taccgaactg
    17221 atggagtggg ctgacgggca actgtccatc actcttaatc tcggtaattt tctgcaactg
    17281 accgacgatc cgctctattt cgatgcttat ctccagtcgc tacaggtggc tgcgatttcg
    17341 acattttgct gtttactgat cggctatccg ctggcgtggg cggtggcgca cagtaagcct
    17401 tcgacccgta atattttatt actactggtg atcctgccgt cgtggacctc gtttctgatc
    17461 cgcgtttatg cctggatggg aatattaaaa aacaacggtg tgctgaataa ttttctgctg
    17521 tggctggggg ttatcgatca accgctgacc attcttcata ccaatctggc agtttatatt
    17581 ggcattgttt acgcctacgt gccgtttatg gtgctgccga tttataccgc gttgattcgt
    17641 attgattatt cgctagtgga agcagcgctg gatctcggtg cacgaccgct gaaaacgttc
    17701 tttactgtga tcgtgccgct gactaaaggt gggattattg ccggatcgat gctggtgttt
    17761 atcccggctg tgggcgagtt tgtgatcccg gaactgctcg gtggcccgga cagcatcatg
    17821 atcgggcgcg tgctatggca agagtttttc aataaccgcg actggccggt ggccgcggcg
    17881 gtagcgatca tcatgttgct gctgctaatt gtgccgataa tgtggttcca caaacaccag
    17941 caaaaaagcg tgggagaaca cggatgaata atttaccggt agttcgttcg ccctggcgga
    18001 ttgtgatttt gctgctgggc tttacctttc tctacgcgcc aatgctgatg ctggttatct
    18061 attcgtttaa cagctcgaag ctggtgacgg tgtgggccgg ctggtcaacg cgctggtatg
    18121 gtgagttatt gcgcgatgac gcgatgatga gtgcggttgg tttaagcctg acaattgctg
    18181 cctgtgcggc aacggcggcg gcgatcctcg ggactattgc ggcggtggtg ctggtgcgct
    18241 ttggcaggtt tcgcggatca aatggctttg cctttatgat cacagcgccg ctggtaatgc
    18301 cagatgtcat cacgggcttg tcgctgttgt tattattcgt cgcgcttgct catgccattg
    18361 gctggcctgc ggaccgcggt atgcttacca tctggctggc gcatgtcact ttctgtacgg
    18421 cttatgtggc ggtcgttatt tcgtcgcgtc tgcgggaact ggatcgctcg atagaagaag
    18481 cagcgatgga tctcggtgcg acgccgctga aagtgttttt cgtcattacg ctaccgatga
    18541 tcatgcctgc gatcatttct ggctggttac tggcttttac tttgtcgctt gatgatctgg
    18601 tgatcgccag ctttgtttcc gggccgggag ccaccacgtt accgatgctg gtcttttcca
    18661 gcgtgcggat gggggtgaat ccggaaatca acgcccttgc aacgttaatt ctcggtgcgg
    18721 tcggaattgt cggatttatc gcctggtatc tgatggctcg cgcagaaaaa cagcggatac
    18781 gcgatatcca gcgtgcaaga catggctgaa gacactaaaa tttgccaacc tggctacata
    18841 atgccgcgca tgccgcggca ttgttttcat ggaagacgaa acgttgggat tttttaagaa
    18901 aacatcttca tctcatgctc gcctgaatgt gcctgcgctg gtgcaggtag cggcgctcgc
    18961 cattattatg atccgtggcc tcgacgtgct gatgattttc aatacgctgg gcgtgcgcgg
    19021 tattggcgag ttcattcatc gcagcgtaca aacctggagc ttaacgctgg tatttttaag
    19081 cagtctggtg ctggttttta tcgagatctg gtgtgcgttt tcactggtga aagggcgtcg
    19141 ctgggcgcgc tggctatatc tgctgacaca aatcaccgcc gcaagttact tgtgggcggc
    19201 ttcgctgggg tatggttatc cggagctgtt cagcattccc ggtgaatcaa aacgtgaaat
    19261 cttccatagc ctgatgctgc agaagctgcc ggatatgctc atcctgatgc tgctgttcgt
    19321 tccctcgacc agtcggcggt tcttccagtt gcaataatgt gtataatcgt cgcccctgat
    19381 gatgtgaagg tcaatgtatg cagtgcgcac tttacgacgc gggtcgctgt cgttcctgtc
    19441 agtggataac gcagccgatt ccagagcaac tctccgctaa aaccgccgat cttaaaaatc
    19501 tgctcgccga ttttccggtt gaggaatggt gcgcgccggt gtcaggcccg gaacaagggt
    19561 ttcgtaataa agccaaaatg gtggtgagtg gtagcgttga aaaaccactg ctcggtatgc
    19621 tgcatcgcga tggcacgccg gaagaccttt gtgactgccc gctttatcct gcctcttttg
    19681 cgcccgtttt tgcggcgcta aaaccgttta tcgcccgtgc ggggttaacg ccctacaacg
    19741 tggcgcgtaa gcgtggcgaa ctgaaataca ttctgctgac tgaaagccag agcgatggcg
    19801 gcatgatgct gcgctttgta ctgcgttctg aaaccaaact ggcacaactg cgtaaggcgc
    19861 tgccgtggtt acaggaacaa ctaccgcaac tgaaagttat taccgtcaat attcagccgg
    19921 tacatatggc gattatggaa ggggagacgg agatctacct gactgaacaa caggcgctgg
    19981 cagagcgttt taatgacgta ccgctgtgga tccgtccgca aagtttcttc cagactaatc
    20041 cggcggtcgc cagccagctg tacgctaccg cgcgcgactg ggtacggcag ctgccagtta
    20101 aacatatgtg ggatctgttc tgcggtgtgg ggggctttgg tttacactgc gcgacgcctg
    20161 acatgcagtt aaccgggatc gaaattgcgc cagaggccat tgcctgtgca aagcagtcag
    20221 ccgctgaact gggcttaact cgtttacaat ttcaggcgct ggactccact cagtttgcca
    20281 ctgctcaggg ggaggtgccg gagctggtgc tggttaaccc gccgcgccgc ggcattggta
    20341 aaccgctgtg tgattatctc tcaacgatgg caccgcgttt tatcatctac tccagctgta
    20401 acgcccaaac tatggcgaaa gatatccgcg aactgcctgg gtttcgcatc gaacgggtac
    20461 agctttttga tatgttcccg cacaccgcgc actatgaagt gctgacgctg ctggtgaagc
    20521 aataaaaaag ccgcaggtgc ggcttcagat tgctgatcag gtgcgctttg ttcatgccgg
    20581 atgcggcgtg tacgccttat ccggcctaca aaatcatgct aaatcaatac attgcagagt
    20641 tgatgtgggc cagataagcg cagcgcatca ggcaatctta cttttgtcat cagtctcaat
    20701 tactgcggga accactggtc attgattttc tgatatgtac cgtcagcttt aattgctgcc
    20761 agcgcgttat tcagtttttc cagcagagct ttgttatccg gacgtacagc gatgcccaga
    20821 ccggtgccga agtattgcgg atcggtcact ttttcagtag caacgcccag ttgcggattg
    20881 gttttcagcc attcgtttac caccgctgtg tcaccaaata ccccatcaat acgaccattt
    20941 ttcagatcga taaaggcatt ctgataactg tcataggaga cagttttcac ttcggggtgc
    21001 tgatcctgaa tatatttctg gtgcgtggtg ccgttttcca tcccaatacg tttgcctttc
    21061 agatcggcaa acgttttgta ggtatctttt ttggcaatca cgacggctga gttttcatag
    21121 tagggcgtgg taaacgatac ctgcttgcta cgctcgggcg tgatatccat accggagatt
    21181 acggcgtcat attttctgaa tttcagtgac gggatcaggc tgtcgaacgc gtgattagta
    21241 aaagtacatt ctgcctgcat ttgtttgcac aaggcttttg ccagatcgat atcaaagccg
    21301 acaatctcat tattagcacc tatagattca aagggtggat aggtggctga aacgccaaaa
    21361 ttgattttct ctgcggcaga agcaccgaaa gtaaaggaag caagtaaagc ggcaagaact
    21421 aactttttca tgatggaact cccgtctgtc aatcttatga tttttggccg tgtctgcggc
    21481 atgggataac aatgccatca agtgaa
//
LOCUS       DAZPEB010000052        20717 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZPEB010000052 DAZPEB010000000
VERSION     DAZPEB010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20717
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.053"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(73..4533)
                     /gene="mukB"
                     /locus_tag="ACHURJ_001730"
     CDS             complement(73..4533)
                     /gene="mukB"
                     /locus_tag="ACHURJ_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFR1280714.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLDGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVVFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVCADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAAYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLVISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGTDEAPSQAS"
     gene            complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="ACHURJ_001731"
     CDS             complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="ACHURJ_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFR1280715.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="ACHURJ_001732"
     CDS             complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="ACHURJ_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFR1280716.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLQVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="ACHURJ_001733"
     CDS             complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="ACHURJ_001733"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFR1280717.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7458..8237
                     /gene="elyC"
                     /locus_tag="ACHURJ_001734"
     CDS             7458..8237
                     /gene="elyC"
                     /locus_tag="ACHURJ_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFR1280718.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTAKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8214..9107)
                     /locus_tag="ACHURJ_001735"
     CDS             complement(8214..9107)
                     /locus_tag="ACHURJ_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFR1280719.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="ACHURJ_001736"
     CDS             complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="ACHURJ_001736"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFR1280720.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10004..10186)
                     /gene="ycaR"
                     /locus_tag="ACHURJ_001737"
     CDS             complement(10004..10186)
                     /gene="ycaR"
                     /locus_tag="ACHURJ_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFR1280721.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10238..11470)
                     /gene="ycaQ"
                     /locus_tag="ACHURJ_001738"
     CDS             complement(10238..11470)
                     /gene="ycaQ"
                     /locus_tag="ACHURJ_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFR1280722.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="ACHURJ_001739"
     CDS             complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="ACHURJ_001739"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFR1280723.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGSKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAEQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="ACHURJ_001740"
     CDS             complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="ACHURJ_001740"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFR1280724.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14275..16547)
                     /locus_tag="ACHURJ_001741"
                     /pseudo
     CDS             complement(14275..16547)
                     /locus_tag="ACHURJ_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16754..17038)
                     /gene="ihfB"
                     /locus_tag="ACHURJ_001742"
     CDS             complement(16754..17038)
                     /gene="ihfB"
                     /locus_tag="ACHURJ_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFR1280725.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17198..18871)
                     /gene="rpsA"
                     /locus_tag="ACHURJ_001743"
     CDS             complement(17198..18871)
                     /gene="rpsA"
                     /locus_tag="ACHURJ_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFR1280726.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18982..19665)
                     /gene="cmk"
                     /locus_tag="ACHURJ_001744"
     CDS             complement(18982..19665)
                     /gene="cmk"
                     /locus_tag="ACHURJ_001744"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFR1280727.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VATFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(19838..20601)
                     /gene="ycaL"
                     /locus_tag="ACHURJ_001745"
                     /pseudo
     CDS             complement(19838..20601)
                     /gene="ycaL"
                     /locus_tag="ACHURJ_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
BASE COUNT         4987 a         5723 c         5032 g         4975 t
ORIGIN      
        1 tatgtgccat ttttttagtg cagaaacgga gttttcggaa aaagaaaagg cggcattgct
       61 gccgccttaa ttttaactcg cctgagaagg cgcttcgtca gttcctggaa gcgtttcagg
      121 gagttgcggc gcaaatcctc gcaggccgac gacatgaaca tgttcggtat tctggaagac
      181 tttacgcacc agtttatagg tggtgccttt ctccgggctg atattttccg gcgctgcgat
      241 gatgagctgc atttgcaaac gctcacacaa ttcaaacagc gtggcgatag aacgagcatc
      301 cagtcgcgct gcttcatcga ggaacagcag acggcaagga gagatatctt taccgcgcag
      361 gcggcgagat tcatcttccc agctttgtac caccatcacc agaatcgaca taccggtacc
      421 aatcgcctca ccggtcgaca atgcaccaga ctctgcgcgc aaccaaccat cggaaccacg
      481 gttaacctca acttccattt ccagatagtt gcggtaatcc agcagttctt cgccaatggt
      541 ctgcggcgtg cgctgcccca tatcaatctg cggattaaga cgctgataca atttcgccaa
      601 cgcttccgag aaggtcagac ggttgctgtt aaacagatcc tgatgctgct cgtgctgttc
      661 agagagcaca tccagcagca tggcgtgcgt ttcacgcacg ttcacgttga ggcgcacgct
      721 gttcacctga ccaaacgata cgttctgcaa cccctggttg agcatacgga tacggttctg
      781 ctcgcgctga atggttttgc gaatgatgtt cgccacgctg cgggaactga tcaccagttt
      841 ctgttcacgg gaggttaatt cttcggtcag acggctaagt tcgatctcca tctgttcgat
      901 agcttccacc ggatcatcgg tacgaataat atcctgacga atacgttcac gcagatgctg
      961 ataagccgcc acgaagaact gaattttacg ctccggacgt ttcggatctt ccgacatgcg
     1021 cagcacgtcg cgcagatgtt cgttatccgc caccgccaga cgcagcgcac ccaatgcctt
     1081 atccgacata gagcgcaaat catcagcgga gagataagcc agctcacgac ggtgtaagcg
     1141 gcgctcaacg ccgttatctt tcaccatgcg catcaccgca caccagcccg ctttggcggt
     1201 cactacctgc tcgcgcatct caaagtaatc ccgctccagc ttgcgcagtt tgcgggtcag
     1261 gttgtccatc tccgcttcgc agaaggtaag cgctttttcc agttgattgc ggcgtgaacg
     1321 gttattgctc agttgcgcat gcagctcgtc acggcgaata cgcgcccgct cttctgcccc
     1381 gctatcagca cacacgccga tatcctgcaa ttcacgttgc agatcgttga gtagctcttt
     1441 tttggtgtcg taagaacttt tcagcgaagc cagcacctgg ttgtactgac tcaactgcgc
     1501 tgcgtgaccg cgcaacgctt cgcgagcgcg ggtacgctcc gcttccgcct gttccagacg
     1561 ttcacgcagt ttttcgttga gatcgctgtt accgctaagc atttctgccg agtcagagta
     1621 gctaaagtgc gcacgacgct gcaccacttc cgtcagggca aacgcctgct gacgggcatc
     1681 gcgctgcatc tgttgagagt acgcgtaatc ttcttttaac tgttcgaact gttccgggtc
     1741 gctctgcaat accgaaacga tcggttccag tttcgccagt tgattgccaa actgctgaac
     1801 aaaacgcgcg gcttcctggg cttcatccag acgttcgcgg atttcatcga cgcgatccgc
     1861 caggctatca tcagccaaca ggttgagacg cggcagaatg cggttcagcg ccgtaacgcc
     1921 ctctttcgcc tgctcaaact gaatacgctg ctgctggtta tcattttcat gattacttaa
     1981 cgcccgctcc agttcgacgc gacggctgtt cagttgacgg atttctgctt ccgggtcaga
     2041 ctcaaacaca accgccagat gactgccgat aaagcggctg aacgcctgat gcagacgctg
     2101 agttttctgt acatcaaagg agagcgtggc gaagcgttcg gaaagcactt cacgctcggc
     2161 atggaggctt tcaatacggc tttcacgcgc agcacgacca aacagcggca cttccgggaa
     2221 acgtgaataa cgccactgac gatcggcgat tttcaccact accgcttttt ccagctcatc
     2281 aacgctgaac acgctgtcat cgaatgactg cggatctcct tcgatcagat agagatcttc
     2341 cgggcaatcg gtcaagccat ctaggtgttc agttacctgt gacagatctg gcaccacgat
     2401 ggcgtggcgt gacgggccat acagcgctga gaagtacggc gcatcttcca ggctaacgtc
     2461 gtcataaatt tctgacagca gcacaccacc aaaacgctcc gccagcgcgt tcagacgctg
     2521 atcttcagag ccgccaggct ggcttaaacg ttcgatctct tcatcgacgg cgtttttgcg
     2581 cgcgcccact tcatcgcgtt caacaatcgc ctctcgctca cgctccagca actgttgcag
     2641 atattcggtg acgtcctggc tggaggtaaa ctcttcgccg cactgttcgc tcaactggtt
     2701 gagactgttt tgcgctgcca gccaaaccgg cgcacgctgc atcaaactct gaatgcgaga
     2761 ctgcagctgt tcctgctcct ggcgcagtgc catgcgctct tcacgggcgt tagacacgct
     2821 atcggaaaga gaggcaatgc gtgcttccag ttcctgatgc agggcttcca gttcgtcgat
     2881 atcaaaattc ttgccctgac gtttgcagaa atctgccagc agacgctcag cttcttgctg
     2941 ctcgcgtaga cgctgttcca gttcgcttaa tcgcatccgc agcggctgaa cctgctctgc
     3001 caggtgacgc tgatcgaccc cttcgcgcaa tagttcgcga gcgacatccc acgcctcgtt
     3061 acgcgccagt gggccgttga ttgccaccac cagctgataa gcctgctcaa actggctgtg
     3121 cgcggtttgc gccatgctca ttttctgctc aagagagagc attttttcag tcgcttccag
     3181 ctctttcgcc tggaaggttt ccagccattc ggcggcgctg tcggcggtta agtccggcag
     3241 atggcacagt tctttggcac gattaagcgc agcaatcgcc tggttatact ggatcgcgcg
     3301 cgtttgctgg acgtccagcg cctgctggta gtcagcaagc tggcttttca gctcgtccac
     3361 ttccagttcg gcagcttccg cgcgagcctc gttctcttcc tggcgttcga tggcttctgc
     3421 caccacttcg ttttgctctt ccagacggat ctgcagctca tcgagatccg cttcgtagcg
     3481 ttcgatcttc tcctgctgac gcagtgcggt ttgcaccagg ttcaggtgat cgctggccgc
     3541 ctgataatcc gcttccagat cgccttcggc accgttgtgc tctgccagct cacgcgccat
     3601 atcgacgtgt ttgtactgct cagccgccag ttgctgacgc gaagtatgta actcgcgacg
     3661 aaactccagg gctttgtcga gatggacacg gcgctcgttg gcgtgacgca tgtagtccgc
     3721 cgccacgtag ttggtagctt cgctgatcag atgcttaaac aggtcacggt ccgactgggt
     3781 gacacgaatc gcttccagcg tcatgcggtt ttcacgcagc gccgcttcca tgtcctggaa
     3841 cgctttacgc acgccgctgt tttccggcaa cagatagtcg cgcagagaac gggtaatggc
     3901 actggagatc ccgccataca gcgaagcttc gatcagacga tagaatttgc tacggtcaga
     3961 tgcggagcgc agacgacgcg cgatgatgcc cagatcgaac atcagcgagt ggtaatcggt
     4021 aatggagttg aactgtttaa actgcacgcc ttccatcgcc tcgagcttgt ctttcagctc
     4081 gttaagcggc agcacgcgcg cctggcgttc gttcagggtt tcggtcacca gctgtgtcgg
     4141 ctgcaccgac atcggcagtc cctgaatggc aaatggtttg atatccactt tacgatcgcg
     4201 tccggcaacc tgttgcagac gcacaccgac caccacgcgc tggtggcgcg agttaatggt
     4261 gtcgagcatc gaataacaga cacccgcttt cagcttaccg tgcagacctt tatcgcgcga
     4321 accgctggtg gccccggctt ccgtagtgtt acggaaatgc agcagggtca ggtcggggat
     4381 cagcgccgta acgaacgccg ccatggtggt ggatttaccc gccccgttac cgccagaaag
     4441 cgtcgtgacc agctcgtcaa ggtcaaaagt tcgggcaaaa aagccgttcc agttaatcag
     4501 cgtcagtgag cgaaatttac cgcgttcaat cattattctt cctctccgct atctggctga
     4561 ttctcttcgg tttcatcgtt gagttgcaga tgattttcaa tcggcattgc ttcgccatca
     4621 cgaatcaggc gacgctgtgc ttcacgggga tcgtcgccag cacgcacatc ggctccgaag
     4681 cggaacaccg attcggtaat gcgaaacttg ctgctgtcgt ggcccataaa ccacaccatg
     4741 cctaaacgac gcagacggtt gagcgaagaa cgtactttct cctgcaactt ctgacggtca
     4801 acgtctgaac cggttgaacg gttgttcacc agtttcagca gttttgcttc atcggccagg
     4861 gtgagcagtt cgtcgtacag ttcctgctgg gtgaaaatcc cctcattcgc cagccgttcc
     4921 gggctgagat agagataaca gaggattttc ccgaccatca tatccagttc cgacaagacg
     4981 gaacgcggga taagagtggt ggaacgtggg cgtaaataga agaacccttc cggtgcgcga
     5041 ataagctcaa cgttataacg cgcgtaaaac tcttccagat attcctgaaa atccatcagg
     5101 aatgcatgat tatccagttc gtcgaggcca atatggcggc ctgaacgtaa ggcgctgtcc
     5161 agcgccggaa ataacggatt cgccagcgcc tgcgccagct taaccggcat cacttgttca
     5221 atatttgtcg atgacatgcg cctgtacctt ggctccgtaa tcattaatcg gctgccattt
     5281 cgctggcagt ccggtgaaat ctgcttgcgc tacgccaaga cgtaccgcct gatcaataac
     5341 aatacgcgca acgtcaaagt gacgtgcacg cggatactgt gacagatatt cgcgtaccac
     5401 cagaccaaga tccagcggca cttgtctggt tttgtacacg gcaagttgtt cttcgatgat
     5461 cgccgccagc tgttcgcgga tctcgttaaa ctcttcgtat tccagatcct caggaagttc
     5521 cccagtcact tcttcatcgc gcagtgccat ctcttcgtca cgcatatcca gcagacgatc
     5581 ggcattggca taagttagcg cccacggctc atcaaaatag gtttgtaccg actgacgtaa
     5641 ccgctgagca aagacgcggt ttttatccat atcgatcgcg gtacgaataa atttgtgtac
     5701 gtggcggtcg tagccaatcc acaagtcgat ggattgctgg ccccaactga taatacgatc
     5761 gagtttgctc tgcagatcga acaccagacg atcgacgaaa tgcagatcgt catgggtcat
     5821 cgtcgcatcc tgaatgcgca acagattagc ctgcaatttg tcgcctgccg cttccagcgt
     5881 atcctgcaat tcacgcagcg ttccggaagt ttccgaaagc aacaattcac agctggaaat
     5941 cgccgcccgc cagtctttgt tcagcaactg ggcgatatcg tccttcacct gctgctgctg
     6001 ttcgtccatc agacgttgcg tcaggtcgat actgtcgaaa atttctgcta ccgaatattt
     6061 cagtggcgca tagacattac ggtgccagtg aaattcatca ccgccctctt cggcggcatc
     6121 tgctgcgcgt ttgagctcac ccgccacaat cgacaactgc atagaaagac gcagcgtaga
     6181 aaactcgcgc tgacggatgt agtagtcagt aatgccgatg ccgagcggcg tcagacggta
     6241 aattgcgttc ccttccgcct gctcgctggt aaagcggttc agcagacgtt gacgcaccat
     6301 gtcgttgatc gcgttattgg cgcgcacgcc gatggtttcg ctggtttgct caaacgcatc
     6361 actcacatgg cggaatgcat ccaccagctc gccttcactc atctcaccat ccagacgctc
     6421 gccgttcagc gtggcaaccg ccagcagaaa agagagtcgg tctacctgca gcgagatgga
     6481 gaagtcattt tttctggccc aggcaaccag ttcggggact gtctgggaaa attcactcat
     6541 actttatcct tgctctgcgg tttgcgcgcg gtgacatgaa tataacgccc cagggtaata
     6601 tacggttcct gacggcaata acgcgtttct aattcaagta atgcttcata gcagtcgcgc
     6661 tgctggtgct tctcgcgcag ataatcatga aacacgcgaa cgcctgtctt acccataatt
     6721 tgccaaccag cttcttccag ccacagataa acctgcgccg ggtcgcgtgg ataatctggc
     6781 gaaagcgtcc gttttttctt tttcggcatt cccgcctgca cgtaatcaaa attcccggcg
     6841 accatgttat gcatcaacaa accatgcgca ttgtagaaca ttaacgacaa cacgccgcct
     6901 ggacgcaaca ctgaccacag ggtctgcaat acactgcggg gatcggccac ccactcgagc
     6961 accgcatgga acaatatcag atcaacgggc gtttccaaat gcgaagcaac atcctgagcg
     7021 gcgcaatgta taaattgcat gttgtcgctc acaccttttg cttctgccgc ctgttttgcg
     7081 cggtcgatca tctgcgcaga aagatcgcat aaaatgacct gatgcccacg ctcggccatt
     7141 ttgattgcgg tctgcccttc tccaccgcca gcatccagca cacgcagttt ttgcgggccc
     7201 atttccgcca gcacgcgatc gagatcctgc cacagaatag cctgtcgaag ctgccctttg
     7261 gtggtgccgt aaatgttacg ggaaaacttt tccgcaatat catcaaaatt gcgatcctgc
     7321 attgctttct ccaccggttg gctaagacgt tttctgataa agacgctatt ttgtcacagc
     7381 cacggcgaga atgaacccgt ctggtgtaat atcgctggcg atcccttgct atatggttaa
     7441 aaaaggaacc agaaaggatg ctttttacac tgaaaaaagt gattggcaat atgttgctgc
     7501 cacttccgct gatgttgtta attattggcg ctggcctggc gctgctgtgg tttagccgtt
     7561 ttcaaaaaac cgcgaagatt tttattagca ttgggtggct ggcactcttg ttattgagtt
     7621 tgcaaccggt agccgaccgc ctgctgcgtc ccatcgaaag cacctatccg acatggaata
     7681 attcgcaaaa agtggactat atcgtggtgc tcggaggtgg ttatacctgg aacccgcagt
     7741 gggcaccgag ctctaattta atcaataaca gtctgccacg cctgaatgaa ggtattcgct
     7801 tatggcgcga aaatccggga tcaaaactga tcttcacggg aggcgtagca aaaaccaata
     7861 cggtgagtac agcggaagta ggtgccagag ttgcgcaatc gctgggcgtg ccgcgcgagc
     7921 aaattatcac cctggatttg ccaaaagata ccgaagaaga agctgcggca gtgaaacagg
     7981 cgattggtga cgcccctttc ctgctggtga cctccgcatc acatctgccg cgggcgatga
     8041 tcttttttca gcaggaaggg ttaaatcctc taccagcccc ggcgaatcag ttggccatcg
     8101 actcgccgct taatccgtgg gaacgggcaa tcccttctcc ggtctggctg atgcatagcg
     8161 atcgtgttgg ctatgaaacg cttggccgca tctggcagtg gctgaaaggt ccgtcaggcg
     8221 agccacggca agagtgattt tgatgccaga tcgaagttgc gccgactaaa tcgtccggta
     8281 ttaaccagtt gcgcaacttc atcccataac acatacaacc aacgccgcca gatgaacgac
     8341 tccgccactg gcgcgcgttg caggtaactc cagagcaaat cttccgccag agaattatcc
     8401 attagtcgga acagttcgta ttctcgcggt gcccaaagca ttagtcccgg cccgaccatc
     8461 gccagtaact gatcgctgcg cgaatctttc agcatgctgc gtaaacagaa gttaccgtga
     8521 atcaacacac aattgtcgtt aaagccttca aataatgccg ggagacattc gcgagtgcga
     8581 aacaggatcc gcttatcctg catcgtcaga ccggtgttat tgaactgatt gagcgtggtc
     8641 catagcactt caacatgttg ccggtaccat gagggccaga aattttcctg agtattgtcg
     8701 accgcgccga cgcaaccgcg actgtcctga cggtgccatg ccagtaaggc ttcaacgatt
     8761 tggtctttga gctgttccca gcgttctggt gttcgggctg gtgcctccac cgaaacacca
     8821 cgcatccgct ccagtagcag gacatcaggg ccggggtgtt cttcatgcgt catcacacca
     8881 taaatagtcg gcatacggac agtcccactt cttgccagca tggtggtttt ccatgccagt
     8941 tgtcgggctt ttccgggcgt actaaagctt cttgccatta acggcattgg gtttccctgg
     9001 ctgtcataca aagcccacaa cgccgtatcc gctttttcat tgacgcactc aatacgactg
     9061 agtttttcgc ccagtaaatg gcttaattcg gcacgcagct gttccattca gattaccctc
     9121 ataaaagcta ctgctttcat aatgagcgcc aatataaaag atgtcacctg aatgagatca
     9181 attgttgaaa agaaaaattg aggaaaatcg taacggtacg acactcctcc caaaattggc
     9241 tgaagtgtcg tgaagtgaaa ttagcgcatt tcagcgcgaa cgcgctcaag atcttcaggg
     9301 gtatccacac ctgtgccagg aacttcctga gcaacagcaa catggatttt ttcgccgtac
     9361 cacagaacac gaagctgctc taacatttcg atgtgttcta acggacttgc ctgccagttg
     9421 acgtaacgac ggataaagcc tgcacggtag ccataaatgc caagatgacg caggaagtta
     9481 tcgccaacgg tttcaaggcc ttctgcaaaa cgatcacgat cccaaggaat ggtggcgcgt
     9541 gagaagtaca gtgcataccc ttcagcgtcg agaaccactt tcaccgcatt cgggttaaac
     9601 gcttcttccg cattgtggat tggcaccgcc agagtcgcca tacccacctg acgctgagcg
     9661 aggttatcag caacctgacg aatgattgtc gcagggatca tcggttcatc accctgcaca
     9721 ttaacgatca ccgtgtcgtc gctgaatgcg catttttcga caacttccgc cagacgttct
     9781 gttcctgact gatgatcggc gcgcgtcata catacttcac cgccagcggc ttcaacggcg
     9841 cgggcaacat cctcatgatc ggttgccacg atgatgcgct cggcacctga ttcacgcgcg
     9901 cgttcaagaa catgaacaat catgggtttg ccgttaatat caaccaatgg tttaccgggc
     9961 agacgcgtgg acgcgtagcg cgcgggaata atgaccacaa aactcatgat ttactctcat
    10021 cagcagtcag cacgcgggct tccgtttcca gcaacactgg aatgccatcg cggagcggga
    10081 aagccaggtt atccagtttg caaataagct cttgtttttc ctggttatac caaagttttc
    10141 cgttgcaaac cgggcaggca atgatttcaa gcaaacgatg atccatagtt cctccagatg
    10201 gatcgggtta tgaatgcata aatcttatca taatcattta tgcgacgggg tctatttccc
    10261 agccagtgcg gcaatcagta aagagacctt gcgggcagcg tcctaatgtc acccgcgttg
    10321 cctgctgcca gctcgcgaaa tcagtaatcg cctgacgtaa ccctttttgc agcattgtcg
    10381 ttggtttaat gccttcctgt aaccacagag agataacttc aaggatgcct gtctggcgat
    10441 gcattttggc atccattcgc ccaactaatt gcccacgatg taataatggc agaacaaaat
    10501 agccatactg gcgtttcggc gctggggtat agcactccag ccggtagcta aaatcaaaaa
    10561 gctgctctgc gcgtttgcga tcccagacaa caggatcaaa aggcgaaagt accgcgctgt
    10621 gcgttgcagt gagctttccg gccagcgcac gctcgagtag cggcagcaaa tcagcatgca
    10681 gccaaagatt gcccaatttt tcaacatgca cagcaatgat ttgctgctgt tcagccctcg
    10741 cttcgcgcca cgccgccagt gccggacgtt tcagccgata gtaatctgcc agccactgtt
    10801 cgcggaatat tcccaggcta cgcgcactgt tatccagcat gatgatttct gcttctgttt
    10861 gcgaaacgag atcgcgctca tcatcccagt caggcatgac acggtgggtt aaatcataaa
    10921 cgcgctggaa gttgcgccgt tcaatcacca tcacctttcc ggcagtaaat aaaccttcca
    10981 gatgccgttt atgcggtttc cattcccacc agccgcttgc acctttacga ggatgctcaa
    11041 aatcggctga acgtaccggc cccttatcat gaatatgctg aattaactgt gcaatttccg
    11101 cctcatgttc ctgcatccag gcgtctttgt atttccagcc cattttttca ggtgccagca
    11161 tgcggtggcg aataagacga aagtcgctac gcggcataaa gcaggcttca tgcgcccagt
    11221 attccattaa ttcgccacgc gccagagact catccagcca ctgggcagga taatttccca
    11281 gacgactgaa aagcaccaga tatggactac gggcaacaat attgatggta tcgatttgca
    11341 gcaaggacat gcgggagatc gttgccggaa tatcctccaa cgacgctcga cggcggggtt
    11401 tgtttaacag gccttgtgcg gcaaggtgaa gattacgcgc atcagcaagg gagaggtgcg
    11461 gcagcgacat tcatgactcc atcaatcgaa cgctgccgcg gcgtaactag ttgccagaag
    11521 ccagcgaggt tagttgcgta agcagtttcg ctggttcatc acctgaaagc tgtgcgtcta
    11581 caggcaaata ccaccaattt tcttctgcaa aggcccggca tttcaccgca tctttttcag
    11641 tcattaccag cgtttgctcg gcgcttacca acgcactgac atccgcatgg ttcaaagact
    11701 gatgatcggc cagcggtaca catttttccg gttgtacgcc acacatcttc agcgtggcaa
    11761 aaaagcgcgg cggatgccca atccccgcca tcgccactac atgttcaagc tgagcaacgt
    11821 cacaacgcgt accggtacgt aaattcaccg cctgacccgg cagcagatgc atgggaattt
    11881 caccgctgcg agggacaccg ccgttgacga ttaccgcatc aaccgacttt aagcgccccg
    11941 ctcgctcacg cattggcccc gccggcaacc accagccatt gccaaagcga cgcacaccat
    12001 caataacgac aatttccaca tcacgcgcca gacggtaatg ctgtaaaccg tcgtcggtta
    12061 cgatgatctg cacatcaggg tgttgcgcca gaatggcttt taccgcatca gaacgaacgg
    12121 gagaaaccgc aacaggcgca tcagtgcgtt gataaatcaa cacaggttca tcacccgcct
    12181 gtgctgttgt ggtatctgcc gacaataaca gcggataaga ttcagcctta ctaccatatc
    12241 cccgcgatac gacccccacg cgaataccgc gctgttgcaa ctgttccacc agccagacaa
    12301 cgaccggggt ttttccgttg ccgcctgcgg tgagattacc aaccacgaca accggtacgg
    12361 gggcacgcca ggcgcgcttc agttttagtt tatagcaaag acggatcgcg ccactcacca
    12421 ggccatacaa ccaggagagt ggcagcaata gccgccacaa aggggattca ccagaccaga
    12481 ttttttcgat cattggccaa actgcatttt gtgaagttgc gcgtaaacgc cgcggtgctc
    12541 aagcaaatcg ttatgcgtac cgcgttccac aatgacacca tcctcgacga ccacgatttc
    12601 gtctgccttt tcaatggtag acaagcggtg ggcaatcacc agagaggtac ggtttttctg
    12661 caactcatcc agtgccgcct gaatcgcacg ttcggattcg gtatccagag ccgaggtagc
    12721 ttcatccaga atcagaatcg ggctatcacg cagcaaggct cgagcgatag caatacgctg
    12781 acgctgaccg ccagagagca gcacgccgtt ttcaccaatc actgtatcga gaccgttatc
    12841 catcttattg atgaagtcca tggcgtaggc catacgcgcc gcttcttcaa tttgctcacg
    12901 gctgtactgt tcagtccgtg cgtaagcaat gttgttagca accgtatcgt taaacagatg
    12961 gacattctgc gacaccagag caacctggtt acgtaacgac gccagggtat actcgcgcag
    13021 atcgtgacca tccatcagga tttcgccttc atcaatatcg taaaaacgag tgatcaggct
    13081 ggcgatggtt gatttacctg aaccagagcg tccaaccaga gcaaccgtct tccctgccgg
    13141 aattttcagg ttgatgttac gcaatgcagg tacgtcacgt cccggataag taaaggtgac
    13201 attgcggaat tccacgtcgc cagtcgcacg ctcgatcacg cgcttacctt cgtctttctc
    13261 ctgctcactg tccagaatgg taaacagcgt ctgacaagcc gccataccgc gctggaactg
    13321 ggcgttaacg ttggtcagtg atttcagcgg acgcatcagt gcaatcattg aagagaaaac
    13381 aacggtaatc gtaccagcag tcaggctatc catgacactt gggaagctcg ccgcatacag
    13441 aacaaacgcc agcgccaaag aggcgatcag ctgaatgatc ggatcagaga tggaagaggc
    13501 tgaaaccatt ttcatcccct gaagacgcat tcggttgctg actttatcaa agcgtttcgt
    13561 ttccacttcc tgaccaccga aaatcaatac ttctttgtgg cccttcagca tttgttcggc
    13621 gctggtggtc acctgcccca tggtgttctg catgttttta ctgatgttgc gaaaacgctt
    13681 cgatactacg cgaatcgcaa tcgaaacaat cggtgccagc acaatcaaaa tgatcgacag
    13741 ttgccaactg taatagaaca tcatgatgaa caggccgatg attgacgcac cttcacgcac
    13801 aaccgttatc agtgcgccgg aagaagaaga agcaacctgt tcggaatcgt aggtaatacg
    13861 tgacaacagc gtccccgttg actgtttgtc aaaaaatgaa accggcattc ccatcatgtg
    13921 accaaacagg cggcgacgca tggtcattac cacctttcct gatacccagg agatacagta
    13981 gctggagaca tagctggtga taccacgtaa aatcatcagc ccgatcacca ccagcggcat
    14041 ccacaccagc acggagcgat ctgttttacc aaagccatca tcaagaagtg gcttaaggag
    14101 cgataacatg aaggtatcgc tggctgcgtt gaggattaac gctacgcccg ccacgatcag
    14161 acccgcttta aaaggcgcaa tggttggcca cagtcggcgg aacgtctgcc acgtagagag
    14221 atctttgtcg ttatgcattc aaaaaaccag catttgttga aatagccgca tattctaccc
    14281 gttatccact ggcacgccaa accactgatg ataccaacga ggtaaaattt gctctctgag
    14341 gctgctaatc cgccagcctt gcgctgaaaa attgaccgtc acttgaccct gatgtggagt
    14401 atcaagccat tgatatcctt gctgttgata gcgatgctta actttattag agggcaatcg
    14461 ccatgcgtta tagcgttata gcgcgatgcc gatgcgagtg ccacttttcc attcactcgc
    14521 tgaattaatg gcaacgatga tgaggtatta ctgccatggt gaggtacctg aagcaatgtt
    14581 gtctgcactt gctgccagta acggcttagc atcttttgtt cagcggggac ttcaatatca
    14641 ccggttagaa gaatgctatt cgtcccgtca taaaccttaa ccacacagga atggttattt
    14701 cctttatcgt tgctagcttg taaaggccag tgcacgctga aacgcaatcc ttgccattgc
    14761 cacgcttcgc cacgcacaca gggctgatga tgttcccagt ttaacggact tctgatccat
    14821 aacatcggcc atgtgtgcaa tattgagtcc agccctcccc ggtgatccag atgttcatga
    14881 ctcagaataa cgccttccgg ttcaagatta tgccagtgga gccaggggat aatcagttgt
    14941 tgcccactat ccccttcggg ccaggccagt cctgtgtcat agagaatcgc tttgccgttt
    15001 ctggctatca ccattgccag cccttgcccg acatcaagca tgtacacctg ccactcgtca
    15061 ggtcgaggtt tttgccacag cggccagcac atcagcaagc ctacagccac acacattgct
    15121 ggcaacgttc gccaggcgtt taatcgccat accaccagta agaaccatgg ggaaaatgat
    15181 agccattgcc aacgttcagc aatgttgatc cacccttctt gcaaactctt taacccccag
    15241 aaaagtaaag ccaaagaccg gtcggcaaga aaccataacc cttgctccag gattaacggc
    15301 ccgcttaaat gcacaaccat cgcggcgagg atcaacggaa ccgtgataaa tgtcaccaag
    15361 ggaattgcta atagatttgc aataaacgag gtcagactaa tgccatgaaa tatgacgatt
    15421 tgcacaggca taagcaggag tgtgattccc agttgcaaat gaatgaggga aacaactgca
    15481 cgcaataccg gcggcagttg ccactcagga cagggaaacc actggtacca aaaaatcagt
    15541 gccgcgaccg cagcggcaga gagccataaa ctttgcgaga gaatggcaac aggatccatc
    15601 agcaaaattg ccgccagaca acatatccat acatcccagc cactccactg tcgcccactt
    15661 aacttaagca ttccccacgt agcaagcgcc accacggtac gcaatgcagg aggttgcatt
    15721 cccgtcaacc aggcataaaa agcagcacag cagattccac caattaatgg catttgccag
    15781 tggatccagc gcccaggcag aaaaacttgc ccaccgcgaa tgagtccggc agccagcaac
    15841 gccgcaaaag cgatgtgcaa tcccgatatc gccattaaat gcgccgttcc agtatcacgc
    15901 atgatatttt tgatttcttt ggggacggat aaccgttccc ccatacctaa accaagaata
    15961 accgcattcc acatataggg ttgcagcgtt gtttgtagtg acgccagata ctgtgcacgc
    16021 aggctacaat tcggttcaat gacacttgcc tgcagaaagc ggccgatgag cggctgatgc
    16081 tgggcaatgg cataacgctg gctatcaaag ccgccatcat taagttggcc atgaactgca
    16141 cgaactttga gtttcattga ccattgttgt ccagcacaaa ccgcttgcgg aagatattcg
    16201 ccatacagca caagacctgg cgcagggaat atacgtcgac cttgtagatg agtaatttga
    16261 ccgtaatgag tggtcatgcc gtcagttgca gtgatctcaa caattgcatc ctgcgtcgcg
    16321 ccagttaagg tttctcctgc ccacaaaatt tgctttgctg ataatatgcc ccaaacgaaa
    16381 aacagcaacg tcagcgcgat ataacggacg gttttaacag ggataaatgc cagtacgcag
    16441 gcgaagagag tcagaaacgc aagggttaat gtcccaggca attggggcaa aatcagcaac
    16501 ggaaaaattc cgcaaattat gcatacgccg actgtcgtta ttttcatgca cccctccctg
    16561 tttatggaaa gtgtgcaact ttgttgagcg gttgtctggt gggaattttg cgttccccga
    16621 ggcacattca gaagaattta acgaaagggt gaaaactgct caaaactctg cgaggcagct
    16681 tccagttcct gattagagaa atgaaaaaag cacccgacag gtgcttttct cgcgttcaag
    16741 tttgagtaaa aacttaaccg taaatattgg cgcgatcgcg cagttcttta ccaggtttaa
    16801 agtgaggaac gtattttcct tccagttcta ctttatcgcc agtcttcgga ttacgtccgg
    16861 tacgtggtgc gcggtagtgc aaagagaaac tgccgaaacc gcggatttca atacgctcgc
    16921 cctgcgcaag agtcgaggcc atatgctcca gcatctcttt tactgcatct tcaaccgtct
    16981 tggcgggaat gtgcgattgc tgggtggcaa gtctttctat caattctgac ttggtcatga
    17041 ttcctccggt tccttaaagg caaattaagc ggctgctgtt gctgcattga ttaagggcgg
    17101 ccgtagccgc ccttagtgct tgattacagg acgaaacctg caatctgtca agtaaactca
    17161 acaaacttcg gaataaaaat cccgaagagt cagagaatta ctcgccttta gctgctttga
    17221 aagcttcagc cattgcgttg ttggagaagt ttgcatcttc ctgtttgtta acagttgcga
    17281 ttgcatcttt ctcgtcagct tcgtctttcg cacgaacaga caggctgatt gcgcggtttt
    17341 tacgatcaac gccggtgaat ttagcttcaa cttcgtcgcc aacgctcaga accagggtag
    17401 cgtcttcaac gcggtcacgg gatgcttcag aagcacgcag gtaaccttca acgccgtcag
    17461 ccagttctac ggttgcgcct ttagcgtcaa ctgcagttac tttaccggtt acgatagcgc
    17521 ctttcttgtt cagagcaacc cagttgttga acggatcttc tgcgagctgt ttaacgccca
    17581 aggagatacg ttcacgttct gcgtcaacct gcagaacaac tgcagcgatt tcgtcgcctt
    17641 ttttgtattc acgaactgct tcttcgcctg caacgttcca ggagatgtca gacaggtgaa
    17701 ccaggccgtc gatgccgccg tctaggccga tgaagatacc gaagtcagtg atagacttga
    17761 ttttaccttc aacacggtcg cccttgttgt gggtttccgc gaactgctgc cacgggttag
    17821 ctttgcactg tttcagaccc agggagatac gacgacgttc ttcgtcgata tccagaacca
    17881 taacttccac tacatctcca acgttaacaa ctttggacgg gtggatgttt ttgttggtcc
    17941 aatccatttc ggaaacgtgt accaggcctt caacgccttc ttcgatttca acgaagcagc
    18001 cgtagtcggt caggttggtc acgcgaccag tcagtttggt accttccgga taacgtttag
    18061 cgatagctac ccacggatct tcgcccaact gtttcaggcc cagggataca cgggtacgtt
    18121 cgcggtcgaa cttcagcact ttaacagtga tttcgtcgcc cacgttgacg atttcgctcg
    18181 gatgcttaac gcgtttccag gccatgtcag tgatgtgcag caggccgtca acgccgccca
    18241 gatcaacgaa tgcaccgtag tcagtgaggt tcttaacgat acctttaact tccatgcctt
    18301 cctgcaggtt ttccagcagc tgatcgcgct ctgcgctgtt ttcggattcg ataacggcac
    18361 gacgagaaac aacaacgttg ttgcgcttct gatccagctt gattacttta aattcaagct
    18421 ctttgccttc caggtgcaga gtgtcacgca ccggacgaac gtctaccaga gaacctggca
    18481 ggaacgcacg aataccgttc agctcaacag tgaagccgcc cttaactttg ccgttgataa
    18541 caccggtaac agtttcagca tcttcgtaag ctttttccag cgtgatccag gcttcgtgac
    18601 gtttagcttt ctcacgggac agcagagttt caccgaagcc gtcttctact gcgtccagag
    18661 caacgtcaac ttcgtcacct acctggattt ccagctcgcc ctgggcgttt ttgaactgct
    18721 cagccgggat ggcggactca gatttcagac cagcgtcaac cagtactacg tctttgtcga
    18781 tagcaacaac aacgccacga acgatagaac ccgggcgggt ttcgatttct tttaaggact
    18841 cttcaaagag ttgagcaaaa gattcagtca tgtttaatct tcaggtttat atttaacgtc
    18901 cacctggctc catgccggat ggggttgttt aacatacccg ctgtcattcc attgcaacgg
    18961 gggtactgca aattcggtcg cttatgcgag agccaatttc tggcgcgcgt attgtagcgc
    19021 tttttcaatc acttgctcaa tgcttaaggt ggtggaatcc aacactaaag catcggctgc
    19081 cggaaccagt ggcgctaccg ctcggttacg atcgcggtcg tcgcgttctt tgatctcggc
    19141 caaaaggcgc tcaaagttaa cactaaagcc cttctcctgc aactgtagca tgcggcgatg
    19201 cgcacgttct tccgaggagg cgtcaaggaa aattttcact ggtgcatcag ggaataccac
    19261 cgttcccatg tcgcggccat cggcaatcag acctggtaat tcgcgaaacg cgcgttggcg
    19321 acgcaataat gcttcacgaa cgcgtgggaa tgtcgcgact tgtgaagccg cattcgccac
    19381 ttcctgagta cgaatttcgc cgctgacatc ttccccttcg aggatcactt ccagattgcc
    19441 attggttgac acaaaacgta catccagatg ggatgccagc ggtaccagcg catcttctga
    19501 cgcaacatca acatggtgat gtaatgccgc caatgccagt acgcgataaa ttgcacccga
    19561 atccagcaga tgccattgca atgcttccgc catagcctta cacaaggtgc ctttccctgc
    19621 accgcttggg ccatcaatgg taataaccgg ggcaattgcc gtcatcttta tctccttaaa
    19681 acaggcgtac cgttaacata accgcgcgca ttatacgcgc cgacgccaca aatcgttaac
    19741 gttgtgtgca aaaaccagtt ttgtctgtat tcagatgggg atttattgcg caattatagc
    19801 gggctggcag aagaccagcc cgaaagatga caatgattta cttaccagag gcgatacgat
    19861 cacggatgtg ttgcgcacgc tctgttgatg gtgggtgaga gtcaaacatg gactgggtgc
    19921 gaccgccatc cagactagcc agtttttcaa agctgccaac cagcccctag gtgctgatgc
    19981 cacgtttctt caacagatca aaggagaaat catctgcttc ggactcttta tcacgggagt
    20041 atttagcatt aatagcgcct tctgcgatat cacccaattg tgagcgggaa agctgggaag
    20101 ccacaccgct ggtagctgaa atggcatcgc gtgcggcaac gatcgcataa gaaactttca
    20161 tttcagccag cgagtgaccc aacgcgacgt ggcccagttc atggcccaga acaccttcaa
    20221 tttcgttgtc attcatcatg tccatcaggc cactgtagac acggacacag ccgttcgcca
    20281 tcgcccatgc gttgacgtcg ctggtcatat aaacctatag ttgaccggcg tgccattaat
    20341 attgttaccc aatgctttgg cgatttttgc cagacgttta ccgtacttgc tggatttact
    20401 tgcgacttga ttgccgctgt ccatttcggc acagccctga ttggcaatcg cttttgcatc
    20461 ggcatcgctt aatgttgctg ctttataggc atttaaaccg gagctgatag ccatattggt
    20521 atcaataccg tgggtatttt gacaccctgt cagtaatgct gcagaggtcg caatcgccag
    20581 cagtaattta gtattcttca tgtgaatcat ccgttgatta atcatatata aacattatgt
    20641 atcaacaact tacattgaca agcgacgcta ccattaatca aaatgactca caaggtccga
    20701 aaaaaaacgc cgacaat
//
LOCUS       DAZPEB010000053        20465 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZPEB010000053 DAZPEB010000000
VERSION     DAZPEB010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20465
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.054"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..880
                     /locus_tag="ACHURJ_001746"
     CDS             <1..880
                     /locus_tag="ACHURJ_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CS1-pili formation C-terminal domain-containing
                     protein"
                     /protein_id="HFR1280728.1"
                     /translation="GDQTSWQLSHTFYNDSFSHKELGVTVGGLNTDTINSAVNGRWDG
                     QYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGASGTDDLLGAVLVDVK
                     GFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVEVNDSSQGNQQGTT
                     NIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKKFPIVGLNSAMLLVAE
                     DGGFTLEINGSEKELYLLSGQQFLKCPLSVEKKRASIRYSGDVTCSVVTYSQLPELKQ
                     PKLRGNVQTAQREVAP"
     gene            877..1966
                     /locus_tag="ACHURJ_001747"
                     /pseudo
     CDS             877..1966
                     /locus_tag="ACHURJ_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CfaE/CblD family pilus tip adhesin"
     gene            complement(2054..3352)
                     /gene="exuT"
                     /locus_tag="ACHURJ_001748"
     CDS             complement(2054..3352)
                     /gene="exuT"
                     /locus_tag="ACHURJ_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFR1280729.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSRGGLA
                     IARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFSLVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQESHNDPAPQH"
     gene            3835..5247
                     /gene="uxaC"
                     /locus_tag="ACHURJ_001749"
     CDS             3835..5247
                     /gene="uxaC"
                     /locus_tag="ACHURJ_001749"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFR1280730.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            5262..6749
                     /gene="uxaA"
                     /locus_tag="ACHURJ_001750"
                     /pseudo
     CDS             5262..6749
                     /gene="uxaA"
                     /locus_tag="ACHURJ_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
     gene            6832..7383
                     /locus_tag="ACHURJ_001751"
     CDS             6832..7383
                     /locus_tag="ACHURJ_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFR1280731.1"
                     /translation="MTAYWLAQGVGFIAFLIGITTFFNRDDRRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTRALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(7388..8632)
                     /gene="sstT"
                     /locus_tag="ACHURJ_001752"
     CDS             complement(7388..8632)
                     /gene="sstT"
                     /locus_tag="ACHURJ_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFR1280732.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDLSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(9248..10213)
                     /gene="alx"
                     /locus_tag="ACHURJ_001753"
     CDS             complement(9248..10213)
                     /gene="alx"
                     /locus_tag="ACHURJ_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFR1280733.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINARVNYRHDKQRGE"
     gene            complement(10497..11483)
                     /locus_tag="ACHURJ_001754"
     CDS             complement(10497..11483)
                     /locus_tag="ACHURJ_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFR1280734.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLLHSKVSDSVLESEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(11562..12245)
                     /locus_tag="ACHURJ_001755"
     CDS             complement(11562..12245)
                     /locus_tag="ACHURJ_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFR1280735.1"
                     /translation="MQPRLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNASKQL
                     TWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKNYDE
                     ASGMQKALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYSIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHSQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            complement(12322..12825)
                     /locus_tag="ACHURJ_001756"
     CDS             complement(12322..12825)
                     /locus_tag="ACHURJ_001756"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFR1280736.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            12910..14046
                     /gene="rlmG"
                     /locus_tag="ACHURJ_001757"
     CDS             12910..14046
                     /gene="rlmG"
                     /locus_tag="ACHURJ_001757"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFR1280737.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTQELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            14331..14645
                     /gene="higB"
                     /locus_tag="ACHURJ_001758"
     CDS             14331..14645
                     /gene="higB"
                     /locus_tag="ACHURJ_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HFR1280738.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNMIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            14642..15058
                     /gene="higA"
                     /locus_tag="ACHURJ_001759"
     CDS             14642..15058
                     /gene="higA"
                     /locus_tag="ACHURJ_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFR1280739.1"
                     /translation="MIAIADILQAGEKLTAVVPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLADLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(15103..17121)
                     /gene="fadH"
                     /locus_tag="ACHURJ_001760"
     CDS             complement(15103..17121)
                     /gene="fadH"
                     /locus_tag="ACHURJ_001760"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFR1280740.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLHTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSESGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELNARRAIAQGTRLALEI"
     gene            complement(17347..19698)
                     /gene="ygjK"
                     /locus_tag="ACHURJ_001761"
                     /pseudo
     CDS             complement(17347..19698)
                     /gene="ygjK"
                     /locus_tag="ACHURJ_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
     gene            complement(19715..>20465)
                     /gene="ygjJ"
                     /locus_tag="ACHURJ_001762"
     CDS             complement(19715..>20465)
                     /gene="ygjJ"
                     /locus_tag="ACHURJ_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HFR1280741.1"
                     /translation="VPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWW
                     GDFTPDLGYVIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYD
                     DKYTYPAMMNGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRAD
                     GLDVPGSESGMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGG
                     RFATAFDSRFYMKNASHDPSDQIFLMQYFYW"
BASE COUNT         5003 a         5357 c         5364 g         4741 t
ORIGIN      
        1 tggcgatcaa acgtcatggc agttatcgca taccttttat aatgattcat tcagccataa
       61 agaacttggc gtaaccgtcg gaggcctgaa caccgatacc ataaacagcg cggttaacgg
      121 gcgttgggat gggcaatacg gaaatgtcta cgctaccgta tctgatagtt atgaccgtaa
      181 gaatcatgat catctttcgg cctttacggg cacttatagc tctacgctgg ctgtcagtcg
      241 ctatggcgtt aatttgggag ccagtggtac agacgatttg ctgggtgcgg tattggtgga
      301 tgtgaaaggc ttctctgaac aggatgaaga gagtcaggat ctgcaactcg aagcgcgggt
      361 ggcaggcagc cgaactttgc agcttggtca aagcgacagt gtgttgttcc cttatcctgg
      421 atttcagtct ggttttgttg aggttaacga cagtagccag ggcaatcagc aggggacaac
      481 aaacatcatt aacggtgcag ggaatcgtga attaatgttg ttgcctggca agctgcgcta
      541 tcgcgaagtg tctgccagct ttaactacaa ctatatcggt cgtttattac tgccggcagc
      601 ggtgaaaaaa ttcccgattg tggggctgaa cagcgccatg ttactggtag ctgaagatgg
      661 cggatttaca cttgaaatta acggcagcga aaaagagctc tatctgcttt ccgggcagca
      721 attccttaag tgtccactga gtgttgaaaa gaaacgcgcc agcattcgtt acagcggaga
      781 tgttacttgt agtgtggtga cttattcaca attaccggag ttgaaacagc ctaaattacg
      841 tggaaacgtt cagacggcgc aaagggaggt tgcaccatga gaaatcgatt gattgcggcg
      901 atattgggct tgtttggcac gctcactggc gttcaggcag ctcctgacgt gaccagtgaa
      961 attacgtatg atttggcatc tggtagagcg gattattact tctggaagga tgaagcgtcg
     1021 gcaggaaata atggatatat gtggtatgaa tgttcgtatc ctgacctcca acaaacctgt
     1081 acagctaatg gaaatatatc gacagtacaa atctatttaa ctgaacaacg cagtgggatg
     1141 cgttggccgg taaaactcaa aggatttaaa acagccattg taagtagcga tgaagcgccg
     1201 ctaggatgca agggggggca aagggcttca gacgaatctt aaggattcta atagatcttc
     1261 atgtacagaa gatggtcaac attattatat atacgataca aagtttctta cgctctacct
     1321 tgagcagaca gagatgaaga atttgccgat tggtggcgtc tggaagggga aagttaaatt
     1381 acattcgaac agcccggccc aggactattt cgcaaatatt accctgaata cgctcgaccc
     1441 caaccatatt gacgtgttct tcccggagtt cgcccacgcc acgccgaggg tgcagttaga
     1501 cttgcatcca acaggaagcg ttaacggcag caactacgcg caagatctga ccatgttgga
     1561 catgtgcctg tacgatggtt ttaacggtaa tgccatcagt tatgaaatca tgctcaaaga
     1621 tgaagggcga ccagctgcag ggcgcagaga cggtgacttc tctatctatc gtcagggagg
     1681 aacctctacc gacgagggag aacgcattga ttaccgggtc aaaatgtaca acccggaaac
     1741 cggtgggcaa attgatgtgc gcaataatga aaatatggtc tggaacagca ttaacctgaa
     1801 aagtgtgcgt ccggtggtac tgcccggtat tcgctatgcc gtgatgtgtg tgccgacacc
     1861 cttggctctg gcagtcgata aattcagcgt gatggataaa caggccgggt actacatggg
     1921 caaattgtca gtaatcttta cgccttcctt gccaaccatc aattaaccct gcaaagcgga
     1981 caaacgcttt tttctatata aacgacgaag ccgcctgata aggcggcttc gtcgttataa
     2041 cttacgttaa acgttaatgt tgcggtgcgg gatcgttatg tgattcctgc gctacctcaa
     2101 tcgccggttt gttctgcaac acggtccaga taaccagcgc acctaacagg tcgaacactg
     2161 ccagtactgc gaacagcggg ctgaagccga tggtgtcagc cagtgcaccg acaaccagcg
     2221 aaaacagcgt acttgccagc catgcggaca tcccggttaa accgttcgcc gtcgccactt
     2281 cgttacgacc gaacacgtca gaagagagcg taatcagcgc gccagacagt gcctggtgag
     2341 caaaaccacc gatacacagc agcataattg cgacatacgg gttggtgaac aggccgatca
     2401 tacccgggcc aatcatcagc actgcaccca gcgttacgac catcttacgg gaaacgatca
     2461 ggttcacacc aaaccaacgc tggaacagcg gcggcaggta accaccgagg atacaaccga
     2521 ggtcagcaaa cagcatcggc atccaggcga acatcgcgat ctctttcagg ttaaagccgt
     2581 aaactttaaa catgaacagc gggatccacg cgttaaaagt accccaggct ggttctgcca
     2641 ggaaacgcgg cagcgcgata ccccagaact gacggttacg caggatctga ccaacggaca
     2701 ttttcttcgc cgtgctcacc tggtgctggg cttcctgacc attaataata tagtcgcgtt
     2761 cttcatcggt cagatgcttc tggtcgcgcg gatgtttata gaaaatcagc catgccatcg
     2821 cccagataaa gctcaatgca ccggagatga taaatgccat ctgccagctg tgcattacga
     2881 ttgcccatac caccagcggc ggcgcaatca tcgcaccaat cgaagaacct acgttaaagt
     2941 aacctaccgc gatggaacgc tctttcgccg ggaaccattc ggagctggct ttcagacccg
     3001 ccgggatcat cgctgcttcc gcggcaccga ctgcaccacg agcaatcgcc aggccacccc
     3061 tgctaccagc cagcgcggtt gcaccacaga atacggccca cagtacagca aacattgcat
     3121 aaccgatttt cgtacccagc acatccagta catagcccgc taccggttgc atgaccgtat
     3181 aagcagcaga ataggctgcg atgatatagg aatactgttg ggtggagatg tttaactctt
     3241 ccatcagagt tggcgcagct gccgccacag tgttacgcgt caggtaacca agcacggtgc
     3301 caagcgtcac cagtgcgatc atataccaac gtaacccttt aattttacgc atgtaaaacc
     3361 tcatcgtttt gttatatccg caggaagcgc ggccatcatc ccgccagagg ctgaagatgt
     3421 tagttacgac cgggtaaccg aaaaaaaacc ggcacgcccc gaacgttgcc atgaaagttt
     3481 cgtgctaatt cggcttccgt accggttaac ccgattcatc taacaaatga atgaatcggc
     3541 aatttgcatt ccgtcgactt atgatttgcg acggcagaaa gatgacttgt catacaactt
     3601 taaaaggtga gagccatcat aaatgtgcga atatttgtag ggacattacc tgacgacagc
     3661 gaggccagta ctggcgcggc ctgcagcgag atttaccact ttgagagtaa tttttttaac
     3721 tacgtttatt gatctaactc acgaaaatat cttcggactc tggaaattgg tgtgataact
     3781 ttgtcagcat cgcaccataa gcaagctagc tcactcgttg agaggaagac gaaaatgact
     3841 ccgtttatga ctgaagattt cctgttagat accgaatttg cccgccgtct gtatcacgac
     3901 tacgcaaaag accagccgat tttcgattac cattgccatt tgccgccgca gcagattgcg
     3961 gaagactatc gttttaaaaa cctgtatgac atctggctga aaggcgatca ctacaaatgg
     4021 cgcgctatgc gtaccaacgg tgtggccgag cgtctgtgta ccggtgatgc gtctgaccgt
     4081 gaaaaatttg acgcctgggc ggcgactgtt ccgcacacta tcggcaaccc gttataccac
     4141 tggacgcacc tcgaactgcg tcgtccgttt ggtatcactg gcaaattgct ttctccgtca
     4201 actgccgatg aaatctggaa cgaatgtaac gaactgctgg cgcaggataa cttctccgcg
     4261 cgcggcatca tgcagcagat gaacgtgaaa atggtcggca ccaccgatga cccgatcgat
     4321 tctctggagc atcacgcaga gatcgccaaa gacggctctt tcaccatcaa agtgctgccg
     4381 agctggcgtc cggacaaagc cttcaatatc gaacaggcga cctttaacga ctacatggcg
     4441 aagctgggcg aagtttccga taccgacatt cgccgctttg ctgacctgca aactgccttg
     4501 accaaacgtc tggatcactt cgccgctcac ggctgtaaag tgtctgacca tgcgctggac
     4561 gtggtgatgt ttgctgaagc gaacgaagcg gaactggaca gcattctggc gcgccgtctg
     4621 gctggcgaaa ccctgagcga gcacgaagtg gcacagttca aaactgcggt actggtgttc
     4681 cttggtgccg aatatgcacg tcgcggctgg gtacagcagt accacattgg cgcgctgcgt
     4741 aataacaacc tgcgtcagtt caaactgctg gggccggatg taggctttga ctccatcaac
     4801 gaccgtccga tggcagaaga gttgtctaag ctgctgagca agcagaacga agaaaacctg
     4861 ctgccgaaaa ccattctgta ctgcctgaac ccgcgcgata acgaagtgtt gggcaccatg
     4921 atcggtaact tccagggcga aggtatgccg ggcaaaatgc agttcggttc cggctggtgg
     4981 tttaacgatc agaaagacgg tatggaacgt cagatgaccc aactggcgca gctcggtttg
     5041 ctgagccgct ttgtcggtat gctgactgac agccgtagct tcctgtcata cacccgtcac
     5101 gaatacttcc gccgcattct gtgccagatg atcggtcgct gggtggaagc gggcgaagca
     5161 ccggcggaca tcaacctgct gggcgagatg gtgaaaaata tttgctttaa caatgcgcgt
     5221 gactacttcg ccattgaact gaactaaggt ctggggttga tatgcaatac atcaagatcc
     5281 atgcgctgga taacgtcgcg gtcgctttag cggatttggc tgaaggcaca gaagtcagtg
     5341 tcgataacca gactgttacg ctgcgccagg atgttgctcg tggacataaa tttgcgttaa
     5401 cggatatcgc aaaaggggcc aatgtcatca aatatggcct gccgattggt tatgcattgg
     5461 cggatattgc ggcgggggaa cacgtccacg cccacaatac gcgcacgaat ctgagcgatc
     5521 tggatcagta tcgctatcaa cctgattttc aggatctgcc tgcgcaagcg gcagatcgtg
     5581 aagtgcagat ctatcgtcgc gctaacggcg atgtcggggt gcgtaacgag ctgtggatcc
     5641 tgccaaccgt gggctgtgtc aacggcatcg cgcggcagat ccagaaccgt tttctgaaag
     5701 agaccaacaa cgctgaaggt accgacggcg tgttcctctt cagccacacc tacggctgct
     5761 cacagctggg cgacgatcac attaataccc gcaccatgct gcaaaacatg gtgcgccacc
     5821 cgaacgcagg cgcagtgctg gtgattggtc tgggctgtga aaacaactag gttgccgcat
     5881 tccgtgaaac gctgggcgat atcgatcctg aacgcgttca tttcatgatc tgccaacagc
     5941 aggatgatga gatcgaagcc gggatcgagc atttgcatca gctgtataac gtgatgcgca
     6001 acgataaacg cgagccaggc aaactcagcg aactgaagtt tggtctggag tgcggtggtt
     6061 ctgacggtct ttctggtatt actgctaacc cgatgctggg gcgtttctct gactacgtga
     6121 ttgctaacgg cggtactacc gtactgaccg aagtgccgga gatgtttggc gcagagcagt
     6181 tgctgatgga ccattgccgc gacgaagcaa cgtttgaaaa actggtcacc atggtcaacg
     6241 actttaaaca gtactttatt gcccatgatc agccgatcta cgaaaaccca tcaccgggga
     6301 acaaagcggg cggtatcacc acgctggaag acaaatcact tggctgtacc cagaaagcgg
     6361 gttccagtgt cgtggttgac gtgctgcgtt acggcgagcg tctgaaaacg ccagggctga
     6421 acttgttaag tgcgccgggt aacgatgccg tagcgaccag cgccctggcg ggtgcgggct
     6481 gccatatggt gctgttcagt actggtcgcg gcacgccata cggtggattt gtgccgacgg
     6541 tgaaaatcgc caccaacagt gaactggcgg cgaagaaaaa acactggctc gactttgacg
     6601 cgggtcagct gatccacggt aaagcgatgc cgcagttgct ggaagaattt atcgacatca
     6661 tcgttgagtt tgccaacggt aaacaaacct gcaacgagcg taacgacttc cgcgaactgg
     6721 cgatcttcaa aagcggcgta acgctataaa gtcgaaatga gttgttaata aaaaacggcg
     6781 tttcataatg tgaagcgccg tttgcttttc catacctgat aaggatttat catgaccgcg
     6841 tattggctgg cccagggcgt gggtttcatc gcctttctga ttggtatcac aacatttttc
     6901 aatcgtgacg accgtcgctt caaaaagcag ctttcggtct atagcgccgt tattggcgta
     6961 catttttttc ttctgggcac ctatcccgct ggtgccagtg ccatccttaa tgccattcgt
     7021 acattgatta ccttacgcac gcgcagctta tgggtaatgg cgatttttat tgtgctgact
     7081 ggcggaattg gcctcgcgaa gttccatcat cctgtcgaac tattgccggt tatcggtacg
     7141 attgtcagta ccagggcgct gttccgctgt aaagggctca ccatgcgctg cgtgatgtgg
     7201 ttttcaacgt gttgctgggt gattcacaac ttctgggcgg ggtcgatagg cggcacaatg
     7261 attgagggta gttttctgct tatgaatggc ttgaatatca ttcgtttctg gcggatgcag
     7321 aaaaggggaa ttgatccgtt taaagtagag aaaacccctc ccgccgtaga cgaaaggggt
     7381 taaacaatta attacgcagg gcgctatttg ccagacgatc gtcttccgcc tggcaagctg
     7441 ccgcagtgaa cagcacgtca gttgaagagt tcagcgcggt ttcgcaagag tcctgcaata
     7501 cgccgatgat aaagccgacg gcaaccacct gcatggcgat atcgttcgaa ataccgaaca
     7561 tattacaggc cagcgggatc agcagcagag aaccccccgc cacgccggat gcgccacagg
     7621 cacacagaga agccaccacg ctcaacagca gcgccgtggg cagatcgacc gggataccca
     7681 gcgtattaac cgcagccagc gtcaacacgg taatggtgat tgctgcgccc gccatattga
     7741 tggtggcacc cagcggaata gaaacggaat aggtatcgcg atccagattc agcttttcac
     7801 acagtgccat attcaccgga atgttcgccg cagagctgcg ggtgaagaag gcatacacgc
     7861 cgctttcgcg caggcacagc agcaccagcg ggaacgggtt acgacgaatt ttccaccaca
     7921 ccagcaatgg gttaaccacc agcgccacca gtaacataca gccaaccagc acgaccagca
     7981 gttgcgcgta gccccacagt gtggagaaac cggtggttgc cagggtagaa gaaaccagac
     8041 caaaaatacc aatcggtgcg aagcgaatga ccagtttcac cataaaggtg acggcattcg
     8101 acagatcgtt gaccaggttt ttggtggtct cgttaccgtg acgcagtgcg aaaccgaggc
     8161 caattgccca caccaggatc ccgatgtagt tacctttcag cagcgcgtcg atggggttgg
     8221 aaaccatgct cattaccagc ccgcgcatca cttctacaat gcctgacggt ggcgaaatat
     8281 cacccgcgct actggataag tgcagggtag aagggaaggc gaagctgaaa acgactgcgg
     8341 ccagagcagc agagaaggta cccagcagat agaggaacaa aatagggcgg atattggttt
     8401 tctgcccgtg ctggtggtta gcaatagatg ccatcaccag catcaacacc aggatggggg
     8461 caacggcttt cagtgcgccg acgaacaaag tacctaacag accaacagct tccgccgcgg
     8521 gttttgagat ccatgccaga agaatcccca gaacaaggcc gaccaggatt tgttttacca
     8581 ggctgccatg agccagacgc cggaatagcc ccggtgaacg ttgcgtagtc atttttcgat
     8641 cctttcattg tgttgtcgca catccctggt gcgttctgtc gacgcatctt tcaggatgta
     8701 acaaagtgtt tgcaaggtcg agtataagga aagttacagc ttcgggaagc gaaaaatgct
     8761 ggattttaaa gagaggtcat attttgcaac ttaattgtta catttgtttg acataaacag
     8821 agggaaggac tggctactaa ggctgattag ataacatatg ggcaaaacta tttggaatat
     8881 gagaacggtt gcgctccttt aatgtgctat gccatataaa gtcactggcg cagggagcgc
     8941 gcagggggca gccaatcgcc gccgccccct gcacccccag gctctggcga acaaaatcgc
     9001 cgctgcgcga ttccctcagc ttatcccttg cggctaccgg gtcgggcgca agggaacgtc
     9061 cctgtaagat gcgccctcgc cgcgcatcca tgcgcgtcgc cccggcctcc agggaacgct
     9121 tcggcgattt tgacgccacc aacacccgtg cggtcttttt gtaaagagct acaagccaat
     9181 tacataatta atagctcacc tttgtctgga atgagttatc tctaggaatg gcttaatata
     9241 cagcgaatca ttctccccgc tgcttatcat gccgataatt caccctggcg ttgataataa
     9301 acgtcatcac cagaatgcca aacaccacgc ccagcgagac ggcgattgga atatggtaga
     9361 agtcgacaat cagcatcttg ataccgataa acaccaggat caccgccagg ccatatttga
     9421 gcatcgagaa acgctctgct acgcccgcca gcaggaaata catcgcacgc aggccgagga
     9481 tcgcaaacag gtttgaggtc agcacaatga acgggtcagt ggtcacggcg aagatagccg
     9541 gaatgctatc cacggcgaaa atcacgtcgc tcaattccac cagaatcagt accagcatca
     9601 gcggtgtggc gtacagcaat ccattcttac gcacaaagaa atgctcgttg tcgatggtgt
     9661 cggtcatgcg caaatgaccg cgtagccagc gcaccagcgg cttgtcgcca atgcctgatt
     9721 catcttcatg ggcgagcgcc atcttcacgc cggtaaacag caggaaggca ccgaagatat
     9781 acaggatcca gtcgaactgc gaaattagcc agctgccagt gaagatcatg atggtacgca
     9841 gaacaatcgc cccgagcacg ccatacacca gcacgcggcg ttgtaatgcc gccggaacag
     9901 agaaatagct gaacaacatc agccagacaa agacgttatc gaccgccagc gatttctcaa
     9961 tcagataacc cgtgagaaag gccagtgcct gtggatcggc aacggcgcga ccttcggttt
    10021 gcaccagata ccaccagaaa gcggcgttaa acagtaacga cagcgtcacc caaaccagcg
    10081 accaggccgc agcctgtttc atggtcatgg catgtgcccc acgacgcccc tgcaacaaca
    10141 ggtcgatagc cagcataatg gcgacaacaa cagcgaatcc gccccatagc aacggcgtgc
    10201 cgacagtatt catagaagtt ccttacacat aaaaaaaacg gccaacgtcg gaagacgtca
    10261 gccgctgctt tttatgcata gacctcgcct tccggcaagg tctcacttac aacaaaaaag
    10321 gaatacgtct gcgtattcct ttcgggttgc ccggtgaccg gatgtggttt ttcacacatc
    10381 gtaatgacga tcgaccggca atgaagttac tcccctttgc aggtaacaaa gtatgacaga
    10441 ccattcagcc agtcaatggt ctgttacata ccttttacac tgttttactt tggcaattat
    10501 agttttacgc tatctgccgg aaaaatcacc ccagtctggc ggcggatctc agtcagcagt
    10561 ttggcggtga tgcgactgac cgccagccct ggatgatcca ccagatgctc atccaccagg
    10621 gtagcaaaca gctctgcttc gtagagcatg gtattaatat gctgcggctg ggtgagatcc
    10681 tgcatctggc tgccacgcgg cacgaagcac actttctggc attcagacag tttttcaatc
    10741 accagcgatc ccgcttcgcc ctgaatctcg ctttccagga cagaatcact gacctttgag
    10801 tgcagcaacg taacgctgaa atcaccgtaa tccatcacca ccacgccgtg ggcatctacg
    10861 ccgcttgcca acaaactggc ggttgcctgc acgcttttcg gttcaccgaa taatgccacc
    10921 gccgacgcca ggcagtaaaa gccgatatcc ataattgaac cgttagagaa tgccggatta
    10981 aaggtgttgg gattctcacc atccaggtaa cgttgatacc gcgaggaata ctggcaatag
    11041 ttgaaaaaga ctttacgcaa tttgccgact ttcggcagcg cctggcgcag caaatgaaag
    11101 ttcggcaggc tggcggtttt aaatgcctca aacagcacca cctgattttc ccgcgcacag
    11161 gcaatggcgg catccacttc cgccagattc gacgccagtg gtttctcgca aatcacatga
    11221 attttatggc taaggaaaag ttgtgtctgg gaaaagtgca gggcattcgg gctggcaata
    11281 tacaccgcgt caatggcatc gctttccgcc atcgcttcca gcgaggtaaa cagatgctcg
    11341 acagaaaaat cattggcgaa gtgctgggcc tgttcaaggc tgcgggaata tacggcggtt
    11401 aacttgtatt taccgctctc atgggcggcc tcgacgaact ggcgagtgat ccagttcgta
    11461 ccaatcacag cgaaacgtat cataacgcgt ggatactcca taaagggatt ctgtcgtcaa
    11521 tgtagcacgc ctttttcatc ttcgacgtgc gcagatacgc attattcttt tgccggacag
    11581 ccatgctcac taaacggccc aatcatgacc gatggcccga gatatttcgg ctggctatgg
    11641 aggattttgg cgtcaatcac ggcgctgaca cgagcgagtt tctctcgcag ttgagtataa
    11701 aaaccgtgct tcatattgag gtcaggcgcg ttaaaaccga tagcgtcgat gctgtactgt
    11761 tttgccagcc agatggcgcg ttggttatga aattcttgcg agataatggt gatatggttt
    11821 tcaccaaaaa cctttttggc acgcactacc gaatccagcg ttgagaatcc ggcatagtcg
    11881 cagaagatca ctttcgccgg tacgccttta gcgatcaaag ccttctgcat acctgatgct
    11941 tcatcgtaat ttttacgtcc gttatcgcca cttaccagta gccatttcac ttttccggcg
    12001 tggtacaacg ctgctgcggt atcaatacgt cgggtaaagt agcgattacc cggcctggcc
    12061 cccagcaata agcccacgtt acgcgccgga actgcgttga catcgctcca ggtcagctgc
    12121 ttgctggcgt tgaccattac tcgatcggcg ataaaaatgg tcgctcctgc cacaagtaac
    12181 agtaagcagg caatctttag agtccggcga gaaaaacaaa tacggagaag aaggcggggc
    12241 tgcatggtcg ttatccttaa cgaaaggggg gaggtcgatt ttaggcgcta aagaaagaaa
    12301 agataacgcc tgacatcatt ttcagatttt gtcctgccca agcgacaact gcgttagcca
    12361 cagccgggtg tcgaactcca gctggtggta ctgcggctcc atatggcagc acaactggta
    12421 aaacgctttg ttatgctctt tctccttcag gtgcgccagt tcgtgcacca cgatcatacg
    12481 taaaaacggt tccggcgcgt tgcgaaacac agtagcgacg cggatctctg cttttgcttt
    12541 cagcttgccg ccctgcacac gagaaacagc ggtatgtaac ccgagcgcgt tttttagcac
    12601 atggatctta ttgtcataca tcactttatt gatcggcggg gcattgcgca gaaactgatt
    12661 ttttagatcc tgagtatatt gccagagggc tttatcggtg gcgtagtcgt gtgttcccgg
    12721 atagcgtttt gccagcacat cgcccagacg ctgttcgtta atcagcgttc gcacctggga
    12781 aagtagctgc tcgggataac cctgaagata agtaagattg ctcataaccg cccacgaaaa
    12841 agagaaaaag ggtatactca cgcacccttt tcaggggcaa cgtcgaaatt ttatcattca
    12901 ggagggccga tgagccactt agacaacggt ttccgttcac tgacactaca acgttttccg
    12961 gcgacggatg acgttaaccc gctacaggcg tgggaagcgg cggatgaata tttgctgcaa
    13021 cagttggacg acacagaaat ccgcggcccg gtgttgatcc tgaatgatgc ctttggtgcg
    13081 ttaagctgcg cgctggcgga acataagccg tacagcattg gcgactcata catcagtgaa
    13141 ctggcgacgc gcgagaattt acgcctcaac gggattgatg agtcgagcgt gaagtttctc
    13201 gacagcaccg ccgactaccc gcaacagccg ggtgtggtgc tgatcaaagt gccgaaaaca
    13261 ctggcattgc tggaacaaca actgcgtgcg ctgcgcaaag tggtcacgtc ggatacacgt
    13321 attattgccg gtgccaaagc ccgtgacatt cacacttcca cgcaggaact gttcgaaaaa
    13381 gtgctcggcc cgaccaccac cacgctggca tggaaaaaag cgcgcctgat taattgcacg
    13441 ttcaatgaac cgccgctggc cgatgcgccg cagaccgtta gctggaagct ggaaggtact
    13501 gactggacta tccataacca tgcgaatgtc ttctcccgca ccgggcttga tatcggcgcg
    13561 cgcttcttta tgcaacatct gccagagaat ctcgaagggg agattgtcga tctcggttgc
    13621 ggtaatggcg ttattggtct gacgctgctt gataaaaatc cgcaggcgaa agtggtgttt
    13681 gtcgatgaat cgccgatggc ggttgcttcc agccgtttga acgttgaaac caacatgcca
    13741 gaggcgttgg atcgcagcga gtttatgatc aacaacgcgc tctccggcgt ggagcctttc
    13801 cgctttaatg ctgtgctctg caacccgccg tttcaccaac aacatgcgct gaccgataac
    13861 gtcgcctggg agatgttcca ccatgcccgc cgctgcctga aaatcaacgg cgagctgtat
    13921 attgttgcca accgtcacct ggattacttc cataaactga agaagatttt cggcaactgc
    13981 accaccatcg ccacgaataa taaatttgtg gtgctgaaag cagtgaagct ggggcgtcgt
    14041 cggtaagatt gggagttacc tgccggatgc ggcgttaacg ccttatccgg cctacgttca
    14101 gcacgttact cccaggccgg ataagacgcg gcaagcgtcg catccggcaa tgtctgccca
    14161 gttctggaat cgcgcttcca taaaacaaaa aatccctctc accatcctct acattctctt
    14221 gtttagcgtt tttctacgtt tattcttccg tcacacagat aaattcatcc gttgccaatc
    14281 tgtcaacgga tgttatcata ttgccaattt gtcaacggac gtgatgaaga atgcacctga
    14341 taactcaaaa agcattgaaa gatgctgcgg aaaaataccc gcaacataaa acggagttgg
    14401 tggctctggg gaacatgatt gctaagggat atttcaaaaa acctgagtca ttaaaagcag
    14461 tattcccatc tctggataac ttcaaatatc tggataagca ttatgttttc aatgttgggg
    14521 gcaatgaatt acgtgttgta gcaatggtct tttttgaatc gcaaaagtgc tacatacgtg
    14581 aagttatgac gcataaagaa tacgatttct ttaccgctgt tcatcgtact aaggggaaaa
    14641 aatgattgcg attgccgaca tcttgcaagc aggagaaaag ctaactgcgg tggtaccttt
    14701 tctggcgggt attcagaacg aggaacaata cacccaggcg ctggaactgg tagatcatct
    14761 gctgctcaac gatcctgaaa accccttgct ggatctggtg tgtgccaaaa taaccgcgtg
    14821 ggaagaatca gcgcccgaat ttgcggaatt taatgccatg gctcaagcca tgcctggcgg
    14881 tatagccgtg attcgtaccc ttatggatca atatggttta acccttgccg atctgccgga
    14941 aattggcagt aaatctatgg tgtcacgcgt tttgagcggg aagaggaaat taacgctgga
    15001 acacgctaaa aaattggcaa cgcgattcgg catttctccc gccttgttta ttgattaacg
    15061 cgacgtgccg gatgcgttgc atccggcgct tcaaataacg atttaaatct ccagcgccag
    15121 ccgtgttccc tgggcaattg cccgtcgtgc gttcagctcc atagccacat cgcagccgcc
    15181 aattaaatgc acagttttcc cgctatcaat cagcggttgc gccagcgcgc ggtttggctc
    15241 ttgccctgcg cagatcacca cattgtccac agctaatacc tgcgtttcgc cgttgatcac
    15301 cacatgcagc ccgtcatcgt caatcttctg ataacttacg cctgggatca ttttcacacc
    15361 ccgcgagagc agggtggtgc gatggatcca gccggtggtt ttgcctaacc cctgtcccgg
    15421 tttgctggct ttgcgctgga gcatcacaat ttgccgtggg ctacgcggga tctgcattcc
    15481 ctgcgggctt aagccaccag cctgttgtag gctgctgtcg atcccccact cattacagaa
    15541 cctggcgata ttctggctgg tggattcgcc cggctgactt aaatacatcg ccgtatcaaa
    15601 accaatcccg ccacaaccga tgatggcaac cttgttgcct accggcgctt tgtcgcgcag
    15661 tacatcgaga taactcaata ccttcggatg atcgatcccg tcgataggcg gaacgcgcgg
    15721 cacgatccca ctggcgagga tcgtttcatc gaacgcctgt aactgatccg ccgtcacggt
    15781 gtgattgagt ttcagcgtca cgcccgtcac ttcgatcatc cggtggtaat agcgcagcgt
    15841 ttcgtaaaac tcctctttgc cggggatctg tttggcgata ttaaactgcc cgccgctctc
    15901 gctgtgggca tcaaacaatg ttacctgatg cccacgcgcc gccgcgttaa tggcaaacgc
    15961 cagcccagca ggtcccgcac cgaccaccgc cagatttttt ttctgcacgg caggaaggat
    16021 tggcattttg gtttcgtggc aggcgcgagg attcaccagg cacgaggtga ctttgccaac
    16081 gaagatctga tcgaggcagg cctgattgca gccaatgcag gtgttgatct catcggctcg
    16141 ccccgattgc gcttttgaca gcagttccgc atcagcaaga aacggtcgtg ccatcgatac
    16201 catatcggca tcgccgcgcg agagaatatc gtcggcaacc tgcggatcgt taatccggtt
    16261 ggtggttacc agcggcagcg agacgtggcc tttcagtttg cgcgtgaccc agctaaatgc
    16321 gccgcgcggc acgggcgtgg caatggtcgg aatacgtgct tcatgccagc caatgccggt
    16381 gttgataatg gtcgcgcccg ccgcttcaat ggcctgcgcc agctctaccg tttcggcaaa
    16441 agtcccgccg tcttcgacca ggtcgagcat cgacagtcgg tagataataa tgaagtcgtt
    16501 gccgacgcgt tcgcgcaccg cacgcactac ttctacggca aatcgcatcc ggttgcggta
    16561 atcgccgccc cactggtcac tacgctgatt ggtgtgcagc gtcagaaatt cgttgatcaa
    16621 atacccttcg gaccccatta cctctacacc gtcgtatcct gcctcccgtg ccagttgcgc
    16681 gcagtgggcg aaatcgtcga tcagttgcag gatctcttca tgggtgagtt cgtgaggaac
    16741 gaaacggttg atgggggcct gcaatgcgga gggagcgacc agatgcggtt ggtagctgta
    16801 gcgcccggta tgcaaaattt gcagggctat tttgccgcct tcctgatgta ccgcttcggt
    16861 aatggtgcga tggtgtggga tctggctggc gtcgttgagc attgcgccac cttccatgcc
    16921 aacgcctgtt aaatctggtg caatgccgcc gctgacaatc agcgccacgc cgtgacgggc
    16981 gcgttcggca taaaacgctg ccagccgctc ggcaccgtcc gggtattcct ccagcccggt
    17041 gtgcattgag cccatcaaca cgcggttttt taacgtggta aaacctaaat ccagcggggc
    17101 gaacagcgac gggtagctca taaaattgtc cagtatgtaa aataattgtt atgtggtcgg
    17161 atgagttcta atttagccgt cgccggagaa aagggaaaag gggagtgcgg ggtttgtgat
    17221 gggattcaaa aagaatacgc ccggtggtaa cgccggatgc gacgcttgcc gtgttttatc
    17281 cggccaggag gattgcacat gtagggcgga taaggcgttt acgccgcatc cgccagcagt
    17341 tgcaaattat tgcttacgga aaaaatcgtt atacaacata tacaaatgcg cggcactcca
    17401 ggagaaattt ggtgcgcctt gctgcgcgcc agtcagcggg ttgtaattct cctgaattgg
    17461 gccatcggcg gttaatcctt tggcgtgccg gaagaacgta tccgccagct tcagggcatc
    17521 atcacgataa ccgtaacgct ccatcccttt cagagcaaac cagaactgat ccacccatac
    17581 gcgcccgcgc cagtagatat cagcgccaaa agccggattg gttaacgccg ccgttcccag
    17641 cgggacaaag gtgttgaact ctttaggatc gagcatcacc ttcaccaccg cgtcggcatt
    17701 ggcctgcgtt gccgcaccgt taaacagcgg cgaccagcct tccggccctt taccgcgctc
    17761 aacaatcggt ttgcccgcgc agccgttcgc cagtggttta tcttcaatac gtacgtcata
    17821 gtagaactgg gtagtcgggt cgaacataca ggtgttgatg tagtccgcga gctgctgtgc
    17881 caactggcga tagcgtttgg cctcttctgg cttaccgagg atggttgcca tctccgccag
    17941 ataatggtta tcgctgtaca tatagctggc ctgatccacc gactcctgca atagcgagta
    18001 gcccagcaac gttccgtcct gactgcggtt ttcggcgaat ttcaccgtcc agtcgctacg
    18061 tttgccacca ttagcgacat atttatccaa ctgttctttg tcgataaaac caaagacggc
    18121 ggcatcatca cgccccgatt cccacgacgc agcgacctgt gccggaattt ccagactgtc
    18181 gtactggcct ttctccacca cgcgggcgta gttgttcagc ccagactgcg tctcttcttt
    18241 gttgcctttc ttcaccgcaa acagcatctc gccgctctca gtgttgtggg ctttgtcgcg
    18301 ggtcgcgcca tattccggca cgccgttgcc gttgtgatcg cggttacgta accaccagtc
    18361 gtgataggcg accagtttcg ggtacatctc tgccaaccag gctttatcct gggtgacgtt
    18421 gtacacttct atcaccgacc aggcggcaag gctgggtttg gtattacgtt cgttccagtt
    18481 gccgccatcg ccaccacgct cggggctaag gttccacgct atcaggtcgg ggacaaagcc
    18541 cacatcctgc ggacgcacgc ggtcgccagg ctggatctgc caggagaaga ccgcgcggat
    18601 attctctttg gcgatgtccg gattgaaatg cgccatcgca aacgcctgct tctaggtatc
    18661 ccacggccag gtctgattgc cggagaacca gcgcccggtc accgacggtg taacggtgtt
    18721 aaatttcacc gcaccgcccg gtgagcgcca gttaccgttg agcgtttcga tcgctttcac
    18781 cgcgacgcgc gtctgttccg gcgtcgcatc cggattggtt aaccctttct tcaggtattc
    18841 ttcccagcgt tgctgcgagg cggtgagata aaacgccgga cgcgccagaa tatcgcggat
    18901 ctgcatttgc tctttgctaa cttcctgagc ggtcagcaga tgggaatagg tggtgtagag
    18961 cgtggtcgaa ccgttgatat gcgccttact ggtaaagcga ttaccgttga tttcagtctg
    19021 caccggcagg gatttatgca cctgatattc cgattcgccg gaggtcagca gatcccaggt
    19081 ggcgcgcact ttgccaaagg tgactttcag gccatcacgg gtggcgctga ttttgcgctg
    19141 atagtcaggg tattcgccag caatggtttt atcggaaagc ggtttccctt ctttcgcttc
    19201 cagtttttcc agcagttcgc cgtcccacac cagatccagc ggtttattgc tggtgatttt
    19261 ggtttccagt agtgacgtgc gcggcgtggc gaagcgcaga gtcatttcga cttgcacatc
    19321 ttttgctgtc agtttttgca ccagcgcacc gggaatactg tatgcctcca gcgtaaagtc
    19381 gactttcttg ccgtcctgcc agacggtcag gcggtcgaaa ttgctggcca taaagttgat
    19441 gtactcttcc gtcagcagcg caacgcccgg aaagccgccc atggtgttag ggccgtctgg
    19501 caacagatga ccatgccagg ctccgagatc gaaaaacgga ttaaaacgct ggtgatcgtc
    19561 gtaatcgtaa tccttcatgt actgcggcgc gccagtacgg ttaatcacgt ttttataatt
    19621 gtcggcgtta gcggcatggg cggaaaaact tatcagcaga gcgcaggtta ctggcgttaa
    19681 aatagttttt attttcatga aattacagtc cttattacca gtagaaatat tgcatcagga
    19741 agatttggtc gctgggatcg tgagaggcat ttttcatgta gaagcggcta tcaaaagccg
    19801 tggcgaaacg tccgccgccg tattcgtacc agataccaaa ctgcatgaaa gaggtggttt
    19861 catcgtcagc gtggtcaggg cgatgtttgg cgtaggtgta agcggtggag agataaacgc
    19921 cctttaacgc ttcatacatg gcgctcacca tcatcccact ttcacttccc ggtacgtcca
    19981 gcccgtcggc gcgtccggta tggtgccagg cgcgagcggc aaagttagga gttagcaggc
    20041 ccgtgagata gagctgtcct gtgccgtcgg tgatttccag accgttcatc caggtgatgc
    20101 cttcggcaag gtcatactgg atgtaaccgt tcatcattgc cggataggtg tatttgtcgt
    20161 catagcgatc gtatttgcca aaatgtagcc aggctttgct ttcatcatga tgtccggcag
    20221 gagtcgcggt gacgctataa cgaaagttgc cgcttaagtt ctgtaccttc agcgcagtca
    20281 taatgtcgcg ggtattggga atgacgtagc ccagatcggg ggtgaaatcg ccccaccatt
    20341 gcaggtcatc cagcgaggaa tcctgacgca gactgagcat gatttcggtg ccatcattgg
    20401 tacgataacc gccatagaaa cggttaattc cgccctcgaa accaccccag ctatggtctg
    20461 gcacc
//
LOCUS       DAZPEB010000054        20406 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZPEB010000054 DAZPEB010000000
VERSION     DAZPEB010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20406
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.055"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            128..943
                     /gene="mepH"
                     /locus_tag="ACHURJ_001763"
     CDS             128..943
                     /gene="mepH"
                     /locus_tag="ACHURJ_001763"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFR1280742.1"
                     /translation="MAQINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            1071..1652
                     /gene="sodB"
                     /locus_tag="ACHURJ_001764"
     CDS             1071..1652
                     /gene="sodB"
                     /locus_tag="ACHURJ_001764"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFR1280743.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(1798..2973)
                     /locus_tag="ACHURJ_001765"
     CDS             complement(1798..2973)
                     /locus_tag="ACHURJ_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1280744.1"
                     /translation="MKINYPLLALALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAA
                     GMLISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSR
                     ILTSLNHGAFFGLGSVVAASVEPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGW
                     RMSFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGA
                     MFTLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYPGGKLADRSVNGTLKGFLL
                     LLMVIMLAIPFLARNEFGAAISMVVWGAAAFAVVPPLQMRVMRVASEAPGLSSSVNIG
                     AFNLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(3139..3228)
                     /gene="cydH"
                     /locus_tag="ACHURJ_001766"
                     /gene_synonym="ynhF"
     CDS             complement(3139..3228)
                     /gene="cydH"
                     /locus_tag="ACHURJ_001766"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFR1280745.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            3527..4552
                     /gene="purR"
                     /locus_tag="ACHURJ_001767"
     CDS             3527..4552
                     /gene="purR"
                     /locus_tag="ACHURJ_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFR1280746.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(4549..5481)
                     /gene="punR"
                     /locus_tag="ACHURJ_001768"
     CDS             complement(4549..5481)
                     /gene="punR"
                     /locus_tag="ACHURJ_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFR1280747.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            5594..6805
                     /gene="punC"
                     /locus_tag="ACHURJ_001769"
     CDS             5594..6805
                     /gene="punC"
                     /locus_tag="ACHURJ_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFR1280748.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKAGNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEAGCQNH
                     GNAEVAHSESH"
     gene            7096..8244
                     /gene="cfa"
                     /locus_tag="ACHURJ_001770"
     CDS             7096..8244
                     /gene="cfa"
                     /locus_tag="ACHURJ_001770"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFR1280749.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSMVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(8284..8925)
                     /gene="ribE"
                     /locus_tag="ACHURJ_001771"
     CDS             complement(8284..8925)
                     /gene="ribE"
                     /locus_tag="ACHURJ_001771"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFR1280750.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            9140..10513
                     /gene="mdtK"
                     /locus_tag="ACHURJ_001772"
     CDS             9140..10513
                     /gene="mdtK"
                     /locus_tag="ACHURJ_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFR1280751.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSVI
                     ILQRASR"
     gene            complement(10554..11810)
                     /locus_tag="ACHURJ_001773"
     CDS             complement(10554..11810)
                     /locus_tag="ACHURJ_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFR1280752.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDGVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            12118..12194
                     /locus_tag="ACHURJ_001774"
     tRNA            12118..12194
                     /locus_tag="ACHURJ_001774"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12152..12154,aa:Val,seq:gac)
     gene            12199..12275
                     /locus_tag="ACHURJ_001775"
     tRNA            12199..12275
                     /locus_tag="ACHURJ_001775"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12233..12235,aa:Val,seq:gac)
     gene            12383..12688
                     /locus_tag="ACHURJ_001776"
     CDS             12383..12688
                     /locus_tag="ACHURJ_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFR1280753.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            12814..14418
                     /locus_tag="ACHURJ_001777"
     CDS             12814..14418
                     /locus_tag="ACHURJ_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFR1280754.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFNRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFVQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(14430..15242)
                     /gene="ydhT"
                     /locus_tag="ACHURJ_001778"
     CDS             complement(14430..15242)
                     /gene="ydhT"
                     /locus_tag="ACHURJ_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFR1280755.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFRRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIAIAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGSGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(15246..16031)
                     /gene="phsC"
                     /locus_tag="ACHURJ_001779"
     CDS             complement(15246..16031)
                     /gene="phsC"
                     /locus_tag="ACHURJ_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFR1280756.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(16028..16696)
                     /locus_tag="ACHURJ_001780"
     CDS             complement(16028..16696)
                     /locus_tag="ACHURJ_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFR1280757.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(16760..17398)
                     /locus_tag="ACHURJ_001781"
     CDS             complement(16760..17398)
                     /locus_tag="ACHURJ_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFR1280758.1"
                     /translation="MNHRDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAELYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(17411..19513)
                     /locus_tag="ACHURJ_001782"
     CDS             complement(17411..19513)
                     /locus_tag="ACHURJ_001782"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFR1280759.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKCVLPAEEYAEIHWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGSPTRETLQR
                     LGLEDIAADLAAHNLLPV"
     gene            complement(19483..20160)
                     /locus_tag="ACHURJ_001783"
     CDS             complement(19483..20160)
                     /locus_tag="ACHURJ_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFR1280760.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITVRILQRKKSWL
                     TVGQVIY"
BASE COUNT         5111 a         5312 c         4763 g         5220 t
ORIGIN      
        1 ctcgccaacg aaacgtattt tttaacaata aaagctatta actttctctt cttctatgca
       61 ttagaatcat caagttttgt aaatcagacg caggcatgat agacctgcct taacagaggg
      121 acgctcagtg gcgcagataa accgtatttc gatcacgctc tgtgctttac tttttaccac
      181 cctgccttta acgcctatgg cccatgcttc aaagcaagcc agggagagtt ctgctaccac
      241 tcatatcacc aaaaaagcag ataaaaagaa aagcacggca accaccaaaa agacccagaa
      301 aacagcgaaa aaagccgcca gtaaaagtac gaccaaaagc aaaaccgctt cttccgttaa
      361 aaaatcttcc attaccgctt ctaaaaacgc caaaactcgc agcaaacacg ccgtcaataa
      421 aacggcctca gccagcttta ccgaaaagtg taccaaacgt aagggctata aatcgcattg
      481 tgtgaaagtc aaaaatgccg cgtcaggaac tcttgccgac gcgcacaaag cgaaggtgca
      541 aaaagctacg aaagtggcaa tgaataaact gatgcagcaa attggtaagc catatcgttg
      601 gggtggcagc tcaccgcgta ccggttttga ttgcagcggc ctggtttatt acgcttataa
      661 agatttggtg aaaattcgta ttccgcgtac ggcgaatgaa atgtatcacc tgcgtgatgc
      721 agcgccaatc gaacgtagtg aactgaaaaa cggcgacctg gtctttttcc gtactcaggg
      781 acgcggcaca gccgatcatg tcggcgtgta tgtcggcaac ggcaaattta ttcagtcacc
      841 gcgcacaggt caggaaattc aaatcacttc tctcagtgaa gactactggc agcgccacta
      901 tgttggcgct cgtcgggtaa tgaccccaaa aacacttcgc taaaacttta ccctgttgtt
      961 acggcaacag ggtaagttca tcttttgtct caccttttaa tttgctaccc tatccatacg
     1021 cacaataagg ctattgtacg tatgcaaatt aataataaag gagagtagca atgtcattcg
     1081 aattacctgc actaccatat gctaaagatg ctctggcacc gcacatttct gcggaaacta
     1141 tcgagtatca ctacggcaag catcatcaga cttatgtcac taacctgaac aacctgatta
     1201 aaggtaccgc gtttgaaggt aaatcactgg aagagattat tcgcagctct gaaggtggcg
     1261 tattcaacaa cgcagctcag gtctggaacc atactttcta ctggaactgc ctggcaccga
     1321 acgccggtgg cgaaccgact ggaaaagtcg ctgaagctat cgccgcatct tttggcagct
     1381 ttgccgattt caaagcgcag tttactgatg cagcgatcaa aaactttggt tctggctgga
     1441 cctggctggt gaaaaacagc gatggcaaac tggctatcgt ttcaacctct aacgcgggta
     1501 ctccgctgac caccgatgcg actccgctgc tgaccgttga tgtctgggaa cacgcttatt
     1561 acatcgacta tcgcaatgca cgtcctggct atctggagca cttctgggcg ctggtgaact
     1621 gggaattcgt agcgaaaaat ctcgctgcat aataactgat ggcaaatgca gcattgcctg
     1681 atgcgcttcg tttaccatgc caacgcggat gaggcgttaa cagcgcattc ggcaagataa
     1741 atcgcacgtt gtcagcaact gtaacgcaga aggttatcct tctgcgtttt tgtttaatta
     1801 gctgttagca acgcaaactg tttcaggttg ttttctggct gacataaaca ccagcaataa
     1861 tgccagtccc gcgacaatcg ctcccatcac cggcacaaag ctgtatccca gcccagcgga
     1921 aattaccgca ccaccagcag ctgctcccag cgcatttcca agattaaagg caccaatatt
     1981 gactgatgaa gacagacctg gcgcttcact ggcgacacgc atcacgcgca tctgtaacgg
     2041 cggtacgacc gcaaaggctg ctgcgcccca caccaccatg ctaatagctg cgccgaactc
     2101 attgcgggcc aggaacggga ttgccagcat aatcaccatc aacaacaaca aaaagccttt
     2161 caacgtgccg ttaactgaac gatctgccag tttgccgccg ggatagttac cgatagagaa
     2221 tccgacacca atcagcacca gcattgccgt gacgaacacc ggtgttgcgt gggtaatact
     2281 ttgcagtacc ggagagatat aggtgtagag agtaaacatt gcaccagctc ccagtaccgt
     2341 cgtcagcaat gcagacaaca cctgcggacg cattaatacc gccagctctt ttttcacttc
     2401 aggtcgtgcc cctgcaccac ctttaggtaa tgagaagaac agacttacca ttgaaatcac
     2461 tcccagcccc gccgttgcca gaaatgacat ccgccagccg atggtttcac ccaaccaggt
     2521 cgccgccggc acgccaccga tatttgccag ggttaacccc ataaacatag tggcaactgc
     2581 gctggcctgt ttatgttttg gctccacgct tgcggccacg actgaaccca aaccaaaaaa
     2641 tgctccgtga ttcaggctgg tcaaaatgcg tgaaagcatc agggtcatat aatccggcgc
     2701 gatggcggaa agtacgttgc cgagcgtgaa aattgccatc aggaaaatca acgcactgcg
     2761 gcgggcacga tgagaaagta gaagcgtcat cagcggcgcg ccaaccatta cgccaactgc
     2821 ataggcactg attaacattc cggcagcggg aatcgagaca tccacaccgc gcgcaatgac
     2881 gggcaacaag cccattggcg agaactccgt tgtcccgata ccaaacgcgc caatcgccag
     2941 cgccagcgcc agcaacggat agttaatttt catgccttat ctccacctct tcgcgtcatt
     3001 acgcgatatt cattaaagtg gcgaaagcat gacagcaatc acaaaaaaat gaaaataaca
     3061 aaaagagaaa acacttttgc cattttgcta acaaacagga aggagatgcg agggagaacg
     3121 cgctccctcg agaggaaatc agtgcagcgc ggcagtcaaa cccacggcca cgatcaaacc
     3181 gaggacgata atcgttgtta ccagtgaaaa tttaaggtcg gtgctcatca agttttctcc
     3241 ttttttatta ccacacaaat agtgatatta cgcattttta cacactgtga tgaaaaaatc
     3301 tcccgtcatt tataatgata agtgttttta ccacttcccc ttttcgtcaa gatcggccaa
     3361 aattccacgc ttacactatt tgcgtactgg ccattgaccc cttcctgacg ctccgtgtcg
     3421 tttttccggc gtaccgcaac acttttgttg tgcgtaaggt gtgtaaaggc aaacgtttac
     3481 cttgcgattt tgcaggagct gaagttaggg tctggagtga aatggaatgg caacaataaa
     3541 agatgtagcg aaacgagcaa acgtttccac tacaactgtg tcacacgtaa tcaacaaaac
     3601 acgtttcgtc gctgaagaaa cgcgcaacgc cgtgtgggca gcgattaaag aattacacta
     3661 ctcccctagc gcggtggcgc gtagcctgaa ggttaaccac accaagtcta tcggtttgct
     3721 ggcgaccagc agcgaagcgg cctattttgc cgagatcatt gaagcggttg aaaaaaattg
     3781 cttccagaaa ggttacaccc tgattctggg taatgcgtgg aacaatcttg agaaacagcg
     3841 ggcttatctg tcgatgatgg cgcaaaaacg cgtcgatggt ctgctggtga tgtgttctga
     3901 gtacccagag ccgttgctgg cgatgctgga agagtatcgc catatcccaa tggtggtgat
     3961 ggactggggt gaagcaaaag ctgacttcac cgatgcggtc attgataacg cgttcgaagg
     4021 cggctacatg gccgggcgtt atctgattga acgcggtcac cgcgaaatcg gcgtcatccc
     4081 cggcccgctg gaacgtaaca ccggcgcagg ccgccttgcc ggttttatga aggcgatgga
     4141 agaagcgatg atcaaggtgc cggaaagctg gattgttcag ggtgactttg aacccgaatc
     4201 tggttatcgc gccatgcagc aaatactgtc gcagccgcat cgccctactg ccgtcttctg
     4261 tggtggcgat atcatggcaa tgggcgcact ttgtgctgct gatgaaatgg gtctgcgcgt
     4321 cccgcaggat gtttcgctga tcggttatga taacgtgcgc aacgcccgct attttacgcc
     4381 ggcgctgacc acgatccacc agccaaaaga ttcgctgggt gaaacagcgt tcaacatgct
     4441 gttggatcgt atcgtcaaca aacgtgaaga accgcagtcc attgaagtgc atccgcgctt
     4501 gattgaacgc cgctccgtgg ctgacggccc gttccgcgac tatcgtcgtt aatcacccgt
     4561 tgcgggagtc tcttccggct cccgcagcca ctccttattc agcgtctcac tatcgccgag
     4621 atactcaagc agccaggtta acgcaggcga catatcattt tgctgccatg tcagacaaca
     4681 tgccgaatcc gggaaggggt tttccaattc aagtgctacc cacttcccct cattaagcca
     4741 cggtttggcg aaatgtgttg gcaccatccc tatgcataat cctgccgaga tacaggttgc
     4801 cgatgattcc cagtcaggca cgacaactct tttttggtta tccagtaacc aggtaatacg
     4861 tttaggtagc gttcgcgagg tgtcttcgcg caccaacgac ggccagttgc gcaacgtatc
     4921 atcgctgaat gggccatcca tcaacgccag cgggtggtgg ctggcaacaa cgcaactcca
     4981 gcttagcatc cccatatccc ggaaggcata acgaccgcct accggaatcg cgcgtgttgc
     5041 gccaatcgcc agttccacgc gcccgtcgga aagcgcatcc cagacaccgt tgaacacttc
     5101 ctgaaagaca agaagttcga catcatcaaa atggcgataa aaatcaacga tcatctgccg
     5161 tgtacgttct ggcctgacaa tattatccac tgcgatagct aactgaccgc gccagccgtt
     5221 cgctatctgc tgacattgct ggcgggtgat ctgcattttt ttgacaacag agcgcccttc
     5281 tttgagaaac cacgctccag cagcggtcag ctccacatca cggtgccgtc gttcaaagag
     5341 cggcaccgcc agccactctt ccagctgacg cacggtatag ctgaccgcag aaggaacgcg
     5401 atgcagctcc tgtgccgcag cgctaaaact accattacgc gctaccgcat caacaacttc
     5461 gagtgaatat tctgaccaca tagtctgcct gcaaaatttt tgaaatcagt catcaaatat
     5521 taccgtttca caacactaat ttcactccct acactttgcg gcggtgttta attgagagat
     5581 ttagagaata tacatgcaac ctgggaaaag atttttagtc tggctggcgg gtttgagcgt
     5641 actcggtttt ctggcaaccg atatgtatct gcctgctttc gccgccatac aggccgacct
     5701 gcaaacgcct gcgtctgctg tcagtgccag ccttagtctg ttccttgccg gttttgccgc
     5761 agcccagctt ctgtgggggc cactctccga ccgttatggt cgtaaaccgg tgttattaat
     5821 cggcctgaca atttttgcgt taggtagtct ggggatgctg tgggtagaaa acgccgctac
     5881 gctgctggta ttgcgttttg tacaggctgt gggtgtctgc gccgcggcgg ttatctggca
     5941 agcgttagtg acggattatt atccttcaca gaaagttaac cgaatttttg cgaccatcat
     6001 gccgctggtg ggtctatctc cggccctggc tcctctgtta ggaagctggc tgctggtcca
     6061 tttttcctgg caggcgattt tcgccaccct gtttgccatt accgtggtgc tgattctgcc
     6121 tattttctgg ctcaaaccca cgacgaaggc cggtaacaat agtcaggatg gtctgacctt
     6181 taccgacctg ctacgttcta aaacctatcg cggcaacgtg ctgatatacg cggcctgttc
     6241 agccagtttt tttgcatggc tgaccggttc accgttcatc cttagtgaaa tgggctacag
     6301 cccggcagtt attggtttaa gttatgtccc gcaaactatc gcgtttctga ttggtggtta
     6361 tggctgtcgc gccgcgctgc agaaatggca aggcaagcag ttattaccgt ggttgctggt
     6421 gctgtttgct gtcagcgtca ttgcgacctg ggctgcgggc ttcattagcc atgtgtcgct
     6481 ggtcgaaatc ctgatcccat tctgtgtgat ggcgattgcc aatggcgcga tctaccctat
     6541 tgttgtcgcc caggcgctgc gtcccttccc acacgcaact ggtcgcgccg cagcgttgca
     6601 gaacactcta caactgggtc tgtgcttcct cgcaagtctg gtagtttcct ggcttattag
     6661 tatcagcacg ccattgctca ccaccaccag cgtgatgtta tcaacagtag tgctggtcgc
     6721 gctgggttac atgatgcaac gttgtgaaga agctggctgc cagaatcatg gcaatgccga
     6781 agtcgctcat agcgaatcac actgatctat atcgatatac ttatacttag gctgctaaca
     6841 aaattttgtt gtatgattga aattagcggc ctatactaat ttcgagttgt taaagctacg
     6901 ataaatatta tgtttttacg gggacaggat cgttcccgac tcactatgga tggtcatttc
     6961 ggcaagggtt cctcctttcc ctctgttcta cgtcggatta tagactcgcg gttttttctg
     7021 cgagatttct cacaaagccc aaaaagcgtc tacgctgttt taaggttctg atcaccgacc
     7081 agtgatggag aaactatgag ttcatcgtgt atagaagaag tcagcgtacc ggatgacaac
     7141 tggtaccgta tcgccaacga attacttagc cgtgccggta tagccattaa cggttctgcc
     7201 ccggcggata ttcgtgtgaa aaaccccgat ttttttaaac gcgttctgca agaaggctct
     7261 ttggggttag gcgaaagtta tatggatggc tggtgggaat gtgaccgact ggatatgttt
     7321 tttagcaaag tcttacgcgc aggtctcgag aaccaactcc cccatcattt caaagacacg
     7381 ctgcgtattg ccggcgctcg tctcttcaat ctgcagagta aaaaacgtgc ctggatagtc
     7441 ggcaaagagc attacgattt gggtaatgac ttgttcagcc gcatgcttga tcccttcatg
     7501 caatattcct gcgcttactg gaaagatgcc gataatctgg aatctgccca gcaggcgaag
     7561 ctcaaaatga tttgtgaaaa attgcagtta aaaccaggga tgcgcgtact ggatattggc
     7621 tgcggctggg gcggactggc acactacatg gcatctaatt atgacgtaag catggtgggc
     7681 gtcaccattt ctgccgaaca gcaaaaaatg gctcaggaac gctgtgaagg cctggatgtc
     7741 accattttgc tgcaagatta tcgtgacctg aacgaccagt ttgatcgtat tgtttctgtg
     7801 gggatgttcg agcacgtcgg accgaaaaat tacgatacct attttgcggt ggtggatcgt
     7861 aatttgaaac cggaaggcat attcctgctc catactatcg gttcgaaaaa aaccgatctg
     7921 aatgttgatc cctggattaa taaatatatt tttccgaacg gttgcctgcc ctctgtacgc
     7981 cagattgctc agtccagcga accccacttt gtgatggaag actggcataa cttcggtgct
     8041 gattacgata ctacgttgat ggcgtggtat gaacgattcc tcgccgcatg gccagaaatt
     8101 gcggataact acagtgaacg ctttaaacgg atgtttacct attatctgaa tgcctgcgca
     8161 ggtgctttcc gcgcccgtga tattcagctc tggcaggtcg tgttctcacg cggtgttgaa
     8221 aacggccttc gagtcgctcg ctaaaggcta ttctatcgcc ccctctccgg gggcgatttc
     8281 agatcaggct tctgtacctg gttgattcat ggcattttct cgtgccgcca gcacacgttc
     8341 taccgtatct accactgcct gagtttgtgg atcaatttca atgttgacgc gtgcgccgag
     8401 ttttttcttc ccaagagtcg tgcgttccag agtttccgga attaaatgga cgcaaaaacg
     8461 cgttggcgtg acttcgccga cggtcaggct aataccgtcg atgccaataa atcctttgta
     8521 caggatatat ttcatcaact gactatcctg gactttaaac cagatctggc gattattttc
     8581 cgaggttaat attttcgcca cttcagcggt ggtcataata tgacctgaca ttaagtgtcc
     8641 gccaatttca tcactgaatt tcgccgcacg ctcaacgttt acccaatccc ccacttttaa
     8701 atcgccaaga ttggtaatgc gtaacgtttc tttcatcagg tcaaaactga catggttgcc
     8761 gttaatttcc gtcacggtca ggcagcaacc gttatgcgcc acggaagcac cggtttccag
     8821 gccgtccagc atgtggtcgg gtaactccac cacatgcgta cgaaaatttg gtttctcgtc
     8881 aatcgacacc agttttgcgg tgccctgtac aatccccgta aacatactta caactcctga
     8941 aatcagttaa gacattctgt tcagcacaat agcaggtggc aaacgccctt accagtgaag
     9001 gggtaagaat gactattttt tcactggaga attaataaat cctcgctaca atagactgaa
     9061 tttcccctgc ttcttctttt tgctgcccat tcaggcggct ttttagtctc tcatataact
     9121 acaaataaaa ggtgttcacg tgcagaagta tatcagtgaa gcgcgtctgt tattagcatt
     9181 agcaatcccg gtgattctcg cgcaaatcgc ccaaactgcg atgggttttg tcgataccgt
     9241 gatggcgggc ggctatagtg ccaccgacat ggcggcggtc gctatcggta cttctatctg
     9301 gcttccggcg atcctctttg gtcacggact gctgctggca ttaacgccgg ttatcgcgca
     9361 attaaatggt tccggtcgac gtgagcgcat tgcgcatcag gtgcgacaag gtttctggct
     9421 ggcaggtttt gtttccgttc tcattatgct ggtgctgtgg aatgcaggtt acattatccg
     9481 ctccatggaa aacatcgatc cggctctggc ggacaaagcc gtgggttatc tgcgtgcgtt
     9541 gttgtggggc gcgccgggct atctgttctt ccaggttgcc cgtaaccaat gtgaaggtct
     9601 ggcaaaaacc aagccgggta tggtaatggg ctttatcggc ctgctggtga acattccggt
     9661 gaactatatc tttatttatg gtcatttcgg tatgcctgag ctcggtggcg ttggttgtgg
     9721 cgtggctact gcggcggtgt attgggtcat gttccttgcc atggtttctt acattaaacg
     9781 cgcccgctcc atgcgcgata ttcgtaacga aaaaggcacc gcgaaacccg atcctgcggt
     9841 tatgaaacga ctgattcaac tcggtttacc gattgcgctg gcactattct ttgaagtgac
     9901 actgtttgcc gtcgtggctc tgttagtgtc tccgctcggt attgttgatg tcgcaggaca
     9961 ccagattgcc ctgaacttta gttcactgat gtttgtgctt ccaatgtcgc tggcggcagc
    10021 ggtaactatc cgcgtaggtt atcgtctggg tcagggctca acgctggatg cgcaaaccgc
    10081 tgcgcggacc gggcttatgg tgggtgtctg tatggcaacc ctgacggcca ttttcacggt
    10141 ttcactccgg gagcaaatcg ccctgttgta caacgacaat cccgaggttg taacgctggc
    10201 tgcgcatttg atgttgctgg cggcggtata tcagatttct gactcaatcc aggtgattgg
    10261 cagtgggatt ttgcgtggtt ataaagatac gcgttccatt ttctatatca cctttacagc
    10321 ttactgggtg ctgggcttgc caagcggcta tattctggca ctgaccgatc tggtcgttga
    10381 acctatgggg ccagcaggct tctggatagg ctttattatc ggcctgacat cggcagccat
    10441 tatgatgatg ttgcgtatgc ggttcctgca acgtctgccg tcagttatca ttctgcaacg
    10501 agcatcccgc taataaagac aaggcgcaac cttcacgggt tgcgcctgtg tttttacgca
    10561 ggctggagcg ttgcgccaat cccgtcttcg tctggctgta atttcagagc gttgcacaga
    10621 gtttgccgac tggcatctgt tatctcaacg tgtatatcac cggtaacttc ccctttcaca
    10681 ttgatgaagt caggtgagat ttctttcgca ctggtttgca ggcaaatcgc tccctctccg
    10741 cgcaggcgtt tcactttcag ttgcacatgc gttgccgcac cagtgatcag ccacagtttt
    10801 ccgccattca gattgatgtt accaacaata aaagtgacac cgtcaccttt taccgtactg
    10861 ccgtcgagaa tcaacacacc gccctgctgt acagtaatcg ttgattgttg cagatctgcg
    10921 ccatagccaa taatcattga agcgccgtcc agtaatgcaa cactcccggt cagttcaacc
    10981 ggtgcgcgat cttcctctac gacaaaattt tcgtccgcgc tggtgggtga caacaggatc
    11041 actgttccac cttgtattac cgcattttcc agcgtaccgg catgcacttc agcccgacca
    11101 cccacattca cggacaggtt ttgcgtctta ccggaactgt agagctgaag gccatccgtc
    11161 cccagacgat aaatggatcc attctcaaca atggtgtcag agtcttcacc accgagatta
    11221 ttcatggtgc caccagcaag caacgtatca ctggctttcc cggccagcat aaaaacgccg
    11281 ccctgattaa tctcacaatt gctggcttcc ccacctggat acaccaacaa ttcaccacct
    11341 tcatctacca cgaccgctga ggttgtccca ttaaccaaca ggccgccctc ttgatcgaga
    11401 atgatttttt ccgcgcgatg tccttccagt acacgcaggt taccgccatt ttccagcaac
    11461 aaaccgcagg cataaccctg atcaacgcta aattcacctt cgggatggcg gccattaacc
    11521 gtagcgagcg tagagagact aattgcgcct ccctcggact gaacaatatc tgtggcaatg
    11581 gcaccgtcgc tgaccgatag cgtgccattg ttaccgtact gtgtgcattt ggcgatccca
    11641 ccggttgcca ctttcaacca gccagccccc gtgactacgg tattttcggc tcttccgcca
    11701 gcttcaacaa tcaactcgcc ttcagtaagt tgcgtcgcgc caatgacctc gccggtctta
    11761 acaatttgca cattcccgtc gctcatcaag tttttcgcat cagatcccat gatttattcc
    11821 tttgctgcat ctgtgtgcct ttattgctac ctaaatgtaa aggctacgga ggatttatcc
    11881 acgacagatt tgagatggtg gcaaacaact ctgtttaaac tctgatacac gaattattgg
    11941 gttgtatcag atgtaaatgc gatcctgaat aaaaatcgcc cttgcaaatc aacaaaatat
    12001 agccaatttg cttaaaagct ctgcaaacgg tcgaaatagc gcagaaaatt acgttttgcc
    12061 tcttgccacc ttcccactct gccgctaata ttcgtccccg ttgtcaccta caacgttgcg
    12121 ttcatagctc agttggttag agcaccacct tgacatggtg ggggtcgttg gttcgagtcc
    12181 aattgaacgc accatcctgc gtccgtagct cagttggtta gagcaccacc ttgacatggt
    12241 gggggtcggt ggttcgagtc cactcggacg caccagattt tcttaatctg gtcttctcct
    12301 ttttccctct gtttcttctc tgtatccaat acgttaaaag atttacactg tcttcgtatg
    12361 cgttatcaga aggagaatcg ctatggcaac tttgttacaa cttcattttg cttttaatgg
    12421 cccatttggc gacgcaatgg ctgagcagct taaaccgctt gctgagtcga ttaatcagga
    12481 acctggtttt ctgtggaagg tatggacaga aagtgaaaag aaccacgaag ccggtgggat
    12541 ctatcttttt actgatgaaa aaagcgccct tgcctatctg gaaaaacata ctgcccgact
    12601 taaaaatctc ggcgttgagg aagttgtcgc caaagttttc gatgtcaatg agccactgag
    12661 tcaaatcaat caggcaaaac tcgcctgaca gaatttaatc aagggcggtt agcgcccttt
    12721 tcatccctgt ctgaaatttc tcaaattcta aaaatctcaa ccaaacttat ctgataacac
    12781 taaattcgaa agaatgcgta caggtaagta acaatgaaaa aaattgctat tgtgggtgcc
    12841 gggcctacgg ggatctacac cttattctcg cttctacagc aacaaactcc actttctatt
    12901 tctatcttcg agcaggctga cgaggccggt gtcgggatgc catacagtga tgaggaaaac
    12961 tcaaaaatga tgctggcaaa tattgccagt attgaaatac cgccgattaa ttgtacgtat
    13021 ctcgaatggc tacaaaagca agaagccagc catctccagc gttatggcgt taaaaaagaa
    13081 accttgcacg atcgtcagtt tttaccgcga attctgctgg gcgaatattt ccgcgatcaa
    13141 tttttacgat tagtagacca ggcacgacag caaaaatttg cagtggctgt ttatgaatca
    13201 tgccaggtta ccgatctgca aattacaaat gctggcgtca tgctcgctac aaatcaggat
    13261 ttacccagcg agacgtttga tttagcggtg attgccacgg gtcacgtctg gcctgatgaa
    13321 gaagaagcaa ccagaacgta ttttcccagt ccgtggtcag gcctgatgga agcaaaggtc
    13381 gatgcgtgta acgtgggtat tatgggaaca tccttgagcg gactggatgc ggcaatggca
    13441 gtggctattc agcatggttc gttcattgaa gatgataaac aacacgtcgt ttttaaccgc
    13501 gataacgcaa gtgaaaagct aaatattacg ttgatgtcgc gcacgggtat tttacccgaa
    13561 gccgatttct attgccctat tccctacgag cccttacaca tcgtcaccga tcaggcatta
    13621 aatgctgaga ttcaaaaagg tgaagagggc cttttggatc gggtatttag attgatagta
    13681 gaggaaatca agtttgctga tccagactgg agccaacgca tagccttaga gagcctgaat
    13741 gtcgattcct ttgttcaagc ctggtttgcc gagcgcaaac aacgcgaccc atttgactgg
    13801 gcagaaaaaa atctccagga agtcgaacgc aataaacgag aaaaacatac tgttccctgg
    13861 cgttatgtca ttctgcgcct gcatgaagcc gtacaggaaa ttgttccaca tctgaatgaa
    13921 cacgaccata aacggttcag taaaggcctt gcccgggttt ttatcgataa ttatgcggca
    13981 atcccttcag agtctattcg tcggctgctg gccttacgtg aagcggggat cattcatatt
    14041 ctcgccctcg gtgaagacta cgaaatggaa attaatgagt cgcgcaccgt cctgaaaacg
    14101 gaagacaaca gctactcgtt tgacgttttt attgatgccc gcggacagcg tccgcttaaa
    14161 gtgaaagata ttcctttccc tgggctacgc gagcaattac agaaaacagg ggatgaaatc
    14221 cctgatgttg gcgaagatta tacgttacag caacccgaag atattcgtgg acgcgtagcg
    14281 ttcggcgcgt tgccctggtt gatgcacgac cagcctttcg ttcagggact tacggcatgt
    14341 gcagaaattg gtgaggcgat ggctcgggcg gtcgtaaaac ctgcatcccg tgcacgtcgg
    14401 cgtctttcgt ttgattaaag gtaaagctat cagcactcaa caaactgatg ctgttcatta
    14461 agacgatagc ggactccggt tcgaatattg ttttcacctt caatggcgac ggcgaagcga
    14521 actctttcgc cgtcatgata tgccagtgcg gcgcttcccc ctgacccgag aataattgag
    14581 tcgaccacgc ccgtgctcac taccgttgaa ttttcaccgc tggcaatgat gtgtacattg
    14641 tcgccgctgt tggcaattct ggcgtttgcg ccaaagctgg cgatttgcac caggtcacca
    14701 ttactggcga catgacaccg ttcacccaat gtacaaacgc gtactcgcat tccggtattc
    14761 gcaatccgac tactatctcc agcgctgctt atccgcgaag aattaccggt tacagcaacc
    14821 cgtgcgcgtt caccggagct gccaatgtga ctgttaaagc cgacacttcc aatccgcacg
    14881 ctataaccca tactggcaat ttgcgcggca tacccagcga ttgcgattcg ggcgttatcc
    14941 tcacaacagc cgatgcgagc atgatcgtcg ctgcgggcaa actgactgca aaatggtatc
    15001 tctccggggt ctgtggccag tttttgcata gaggaaactt cctgatgcgc gaagttttca
    15061 tccgccagcc attttttcca cgcatactca accagactct ccgcccagtc cgtatatcct
    15121 tcctcaatca gtgcatgatg aatatcggca taactaccgc cgcgtcggaa atggcgcagg
    15181 aaccagcgat acatcaccgc tccgatacgc cattctcgca ggtctgctcg tgtaatgatc
    15241 attcgttagt gccggtgata gccatcgacc atgcttttaa aggtttcgtg tggcgtacgc
    15301 cccgtggtgc aaagataaag atgtccgaag ataaaaaaga ggcttataaa tgccagagca
    15361 aaatgcgcct gcaataacca gtatcttacg ccaggaaaca catctcccac ggcttgcgga
    15421 tagagacaca acagccccgt caatagtaac aacggcagca atccatacat gacaccaaca
    15481 taggcgacct gctgtaaggg attaaattta gactgtgttg ttgccgggaa aggatgttct
    15541 tccccctgca taatgccaaa caaataaaat cgcgtttgtt ttgccgctcg ttccagccac
    15601 ccctgacgac gaatgcgata gtggtgacca ttatccccaa cggcattgat cagcacaaag
    15661 ccgagccagc atgccagtaa caaaaatccg caaacttcat gcaccgcaac cagactttta
    15721 accgcagttg cgcccaccat cgcaaagtga tttatcagcc cacttgccag caacaataca
    15781 aagagtaacg cattcgacca gtgccataat ctgaccgctt tgctgtaaag ataaaccttc
    15841 tcaccatgat cggtggctga tttcttcacg ccacgagcac gaagcaaggc gtgcagcccc
    15901 agcaccaacc acatcccaac cagcaatact ccggcaatga tcaaccacac cggccaaaat
    15961 tcgggagtga actgtggcac atagttcgct aactggctct gaaactgttc agcatgttgc
    16021 gacgggttca tacattttcc tttttaatca aatgttgacc gaaccggcga tacagatgcg
    16081 gtttcccggc accaggtagc tgatattgat agtatttatt atcctgcaac cacgcctgaa
    16141 tttctgggct atcttcgcga ccaaagatca atgcgtgttc cggacaggcg ctgacgcaaa
    16201 tgggcggaaa gccttttgcc agtcgggact cagcgcagaa atcacattta tctgccactt
    16261 tagtgacagg gttaaggtag cgaacctggt acggacaagc gccaatgcaa taactgcagc
    16321 cgatgcactg cgatttttct acccgcacga tcccctgctc atcacgccac gatgcccccg
    16381 tcgggcaaac gtcgatgcac ggagcatctt cgcagtgctg acatgactgg cgaaaaaagt
    16441 gatactgcgt ttcgttgtcg ttatcggtga ctggaatgtg tgctatcgat aaacggcttc
    16501 cctgagcggg gacgtggtta gttttacggc atgcgcgggc gcaaatatta cagccattgc
    16561 accgtgactc gtcatgaatc atggcatacc gaacttcctt ttcttgcctc gtgttcgcta
    16621 acagagaaga cgcagaacca gtaaaaaaga ttactgttcc catccccaga acaaatttgc
    16681 gtcgagtgaa ggacatctga ttatccccta atgcagtggc tgcaactgtc caccctatcc
    16741 atgacatctc cctgaaattt caggggttct gaaactgctc tacctcgaac cattcgcata
    16801 agccataagc cttgccatag gtcataaatc gctcactaaa caagtacaac tccgccgtcg
    16861 atgactccac ctgccgtata tcggcatatt caggtgcaac atccatcgcg gcgatcgcgg
    16921 cttcaatctg cgcttcctgg aagtaatatg gcgcttgcat cagcatggcg actttgtaag
    16981 gacgaggata tgtctggcac tcaaaacgaa cggcgtgtgc aatggcgcga caaatgtcct
    17041 gctccacaac ttgcaaagac atcgccgcgt agttttcact catggcctgc gttgagtaat
    17101 agtaatcatc ctggcttccc gtcatacaag cgatatcagc gtagtctccg ttctgcttaa
    17161 tgctttccag caaaactgac agctcctctt ctgcaaccga atagggtggc gagagaaaca
    17221 cggcgcgcgc caccagctgc ccagccgcag aatgctggcg aatgaactct gccaggattt
    17281 cagccggttc tggttcggtt accgtatcaa cggggtgacg catcccctgt aaccattgcc
    17341 gcttcgcctc gtcaacttcg ctgactttcg ctaacggcaa ttcatcccga tggttcatct
    17401 cacccattcc ttacacaggc agtagattgt gtgctgccag atcggcggca atatcttcca
    17461 gccccagacg ctgcaatgtt tcgcgggtgg gactaccaag ctgtgggtcc cagcccattt
    17521 ctttgtagaa catcgtgagc gaagcatgca tatcgtcacg atccattttg tcagtacctt
    17581 cagtaaagac cgggatctgc ggatccttgt cgaataccca ggaacagata agatcgtgtt
    17641 cattacgcat atctttggtt tgcataagtt ttaccgtgta ggcacgatgc aacgtaaaga
    17701 tacgctctgc agctaaatct aatttttcct gagtcatctc ttcgccggtg atcgctttga
    17761 agaatttggc ttcaagcgcc agatcgcccc ggtaattacg gcttttcagc ggcgaaacgg
    17821 tcattggcca gacccagtta cacagggtga cggcgttatg caaacagacc cgcaacagcg
    17881 accacttagc atatttaatt tttgcgtcat tgattggcgt gtagtttttg gtttcatcgt
    17941 aggcatcttc agaaccaaaa agctctttcg ccacttcacg ttgcagtttc aatggcaagc
    18001 cggaaccaat aaagttgata tgggtatgtg tcatgcagtc acggttgaac atacagttaa
    18061 caatggaacc gacctgcgct gacgcttcat tggcatggtg aaccggatag ccaaacggcg
    18121 accagagttt atttttcgcg tagccccagt actcttcacc caaattccag cgttctgcga
    18181 tggcatatga accatcagcc aggtgactca gctcacccac acgatgcgcc agacggtagt
    18241 aaaaatcttt aatgaaatta acgtcacccg cttccagttg atcccagtga atttccgcat
    18301 actcttcagc aggcagaaca cacttgaaca cccctttgct gtaacaataa gtaaagtcgc
    18361 gatgcaactg cccgtagtta caccataggc cgtagtcatc gaacaaattc agaccaacca
    18421 agttaccaat cacacggcca tcgtctttat cttcaaaatc tttcggaccg ttcgggaaga
    18481 tggtggtatg gacaaagttg gctacacaag tgttaccacc tgtgctgggc acgccaaact
    18541 ctttcacccg aggaatattc atttgggtca tacaacggat cgggcaagag tggcagccac
    18601 tcatttttac tgtgtatttc tctgccgccg gacctaagtc aaaaacagat ttataggtac
    18661 gaaagccgac cgtgttctga ttgcctggcg gaatttcccc cgtttcaatc ggaccacctt
    18721 cagccgcgcc ccaaaacagc cctttacgtg ctgtccaacg tgacttggga tctgaatact
    18781 ctgcccacga ttgcggcgta cttggcacga catggttgtt attcgcacca ataagttcag
    18841 tcatcatgta atcgttgaga cgcttcatct cctgacgatc ggcaatgttg acccctttcg
    18901 tcccttcaac cgcaatcgct ttcaggtttt tcgaacccat aattgcgcca gttcccgcac
    18961 cgccgctgtg gttacggcta ttcaacatgc cagagagagg aacaaggttt tccccagcct
    19021 ggccaatagc cgccacacag gtttccggac tggtcaatcg acaaatttct tccgtcgttg
    19081 cgcgcgtccc ttttccccat aagaaatcgg ctttttccag gctaactttg tcatctttaa
    19141 tcttcagcca taccggtgat ttcgccttcc cttcgataat aatgacgtcg tatccagcga
    19201 atttcatttg cgctgcaaaa aagccaccca tatgggcatc gacaactaaa tttcctttgg
    19261 taaaagtaga aagtgaggtg atatttacgc gagaactaca gggggcacca gatccagtta
    19321 atgggccggt agcaaagact aatttattcg cttcatcgaa aggtttcgtg cctggcggta
    19381 cttcgtcata cataattttg tagccgaagc ccatgccacc gacaaaactt ttaaacttac
    19441 tggaatcttc gagggtaata tttcctgtcg tgagattgac tcttaatata ttacctgtcc
    19501 aaccgttagc catgattttt tcctttgcaa gattctcaca gtaatatctt tccactcgat
    19561 aatttttaac gcccctgttg ggcaggcgtt tgcacattca ccgcataaca cacatttcga
    19621 ggatttttta ctttcggtat ttacggtggc catcatccac gggcatgccg tagtacaggc
    19681 gctacagcca atacagcgtt tatgatcgac ggtaatacag ccttctttct gctgccaggt
    19741 aatcgcacca atcgggcaga cgttcatgca ttgcggctct ttgcattgac ggcaggtgtc
    19801 cgcggtatag ttgagatcgc catacaggcc gccgccagag ccaaccccgt tgtcgccaaa
    19861 gaaataattg cgatggattt tgatacggga gaagaatgtc cctactgagc catcgttgaa
    19921 gttggtacaa gagatttcac agcggtggca acccgtgcat cgcgctcgct gggtcaccag
    19981 cacccctttg ggtgtattta tcaaccctac tgtgccgcta tcaatatctt cctgtttgca
    20041 acccaaaagt gacagtaacg caggggcaat tgttagacca gcaaggcctt ttcctgatat
    20101 acgcaagaat tctaatcgcg ttaagccaat atctaatagt ggacgatcaa ccgggttcat
    20161 ttatttcgca tcctcttcgc aaacacagtg ctgacaattc agcagcctga tattaagcat
    20221 tcaattaata cgctgctgac tctgtcccca acaccagata ttttccctaa cccgatagtg
    20281 tatgagaaaa gatttaacgc cgataaacta atcctttatg gttaggagaa aaaataatgt
    20341 gattattctg agcccttaac attgatcgtt atcaattaaa attacaaagc aagaaattat
    20401 aagtga
//
LOCUS       DAZPEB010000055        20351 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZPEB010000055 DAZPEB010000000
VERSION     DAZPEB010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20351
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.056"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(56..481)
                     /gene="nudI"
                     /locus_tag="ACHURJ_001784"
     CDS             complement(56..481)
                     /gene="nudI"
                     /locus_tag="ACHURJ_001784"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFR1280761.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEYGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYTWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            760..1302
                     /locus_tag="ACHURJ_001785"
     CDS             760..1302
                     /locus_tag="ACHURJ_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFR1280762.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            1402..2604
                     /locus_tag="ACHURJ_001786"
                     /pseudo
     CDS             1402..2604
                     /locus_tag="ACHURJ_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
     gene            2824..3606
                     /locus_tag="ACHURJ_001787"
     CDS             2824..3606
                     /locus_tag="ACHURJ_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFR1280763.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDHRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLSWQPAAVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYPDVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKSIACARDISRLLGWKSPFDLQAS"
     gene            3621..4826
                     /gene="rhmD"
                     /locus_tag="ACHURJ_001788"
     CDS             3621..4826
                     /gene="rhmD"
                     /locus_tag="ACHURJ_001788"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050032 - L-rhamnonate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HFR1280764.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQSRQSFGINVLGTLVVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKRNAPVGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            4883..6172
                     /locus_tag="ACHURJ_001789"
     CDS             4883..6172
                     /locus_tag="ACHURJ_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1280765.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVMAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFA
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            6190..6993
                     /gene="yfaU"
                     /locus_tag="ACHURJ_001790"
     CDS             6190..6993
                     /gene="yfaU"
                     /locus_tag="ACHURJ_001790"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HFR1280766.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTSYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVSSATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(7034..7936)
                     /locus_tag="ACHURJ_001791"
     CDS             complement(7034..7936)
                     /locus_tag="ACHURJ_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFR1280767.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(8129..9319)
                     /gene="glpC"
                     /locus_tag="ACHURJ_001792"
     CDS             complement(8129..9319)
                     /gene="glpC"
                     /locus_tag="ACHURJ_001792"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFR1280768.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(9316..10575)
                     /gene="glpB"
                     /locus_tag="ACHURJ_001793"
     CDS             complement(9316..10575)
                     /gene="glpB"
                     /locus_tag="ACHURJ_001793"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFR1280769.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLH
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLLCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPHFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(10565..12193)
                     /gene="glpA"
                     /locus_tag="ACHURJ_001794"
     CDS             complement(10565..12193)
                     /gene="glpA"
                     /locus_tag="ACHURJ_001794"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFR1280770.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            12466..13824
                     /gene="glpT"
                     /locus_tag="ACHURJ_001795"
     CDS             12466..13824
                     /gene="glpT"
                     /locus_tag="ACHURJ_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFR1280771.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            13829..14905
                     /gene="glpQ"
                     /locus_tag="ACHURJ_001796"
     CDS             13829..14905
                     /gene="glpQ"
                     /locus_tag="ACHURJ_001796"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFR1280772.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(14947..15189)
                     /locus_tag="ACHURJ_001797"
                     /pseudo
     CDS             complement(14947..15189)
                     /locus_tag="ACHURJ_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            15184..15288
                     /locus_tag="ACHURJ_001798"
                     /pseudo
     CDS             15184..15288
                     /locus_tag="ACHURJ_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15368..16018
                     /gene="inaA"
                     /locus_tag="ACHURJ_001799"
                     /pseudo
     CDS             15368..16018
                     /gene="inaA"
                     /locus_tag="ACHURJ_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416740.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
     gene            complement(16072..16326)
                     /gene="yfaE"
                     /locus_tag="ACHURJ_001800"
     CDS             complement(16072..16326)
                     /gene="yfaE"
                     /locus_tag="ACHURJ_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFR1280773.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(16326..17456)
                     /gene="nrdB"
                     /locus_tag="ACHURJ_001801"
     CDS             complement(16326..17456)
                     /gene="nrdB"
                     /locus_tag="ACHURJ_001801"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFR1280774.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDSEVDTDDLSNFQL"
     gene            complement(17603..19888)
                     /gene="nrdA"
                     /locus_tag="ACHURJ_001802"
     CDS             complement(17603..19888)
                     /gene="nrdA"
                     /locus_tag="ACHURJ_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFR1280775.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINNLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGECP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            20016..20159
                     /gene="ypaB"
                     /locus_tag="ACHURJ_001803"
     CDS             20016..20159
                     /gene="ypaB"
                     /locus_tag="ACHURJ_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFR1280776.1"
                     /translation="MALLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
BASE COUNT         4755 a         4959 c         5493 g         5144 t
ORIGIN      
        1 aattcgttaa ccactaaatt ttcaatctga cagccagtga tggctgtcat cattgttaaa
       61 gaagaccttt caaacgtaac gtttttcggg tggcaacatt caaatcataa tgcaccagat
      121 cttcaggttt tacccacgtg tagtcctgaa actcttcgtt tattttcact tctcggttgg
      181 cagaaacgca gtcaaaaatc aggtaaatca tataaatctc ttccttgcga ccatctgcat
      241 acgtcttggt gcgaatatca tcgctgaagg tccacggcgt gatttctgtc aaaagcagct
      301 gttctcccag ttcttcgcga atttcgcggc gtagtgcctc ttcaattcgt tcgccatact
      361 ccacgccgcc acccgaaagc gcccattgac cggggaaaac gccgcgatcg tcggccattt
      421 tgcacagcag ataagcacca tcattttgaa tcaaagggca tacaatagtc cgttgtcgca
      481 caggggtatt ccttataaat aaaaagaaac catgagaata cggtaactca gaaatatgtg
      541 aaagtgacag taatctcttt tgcgatgata actgcactta ttattatttt tgtgtgtatc
      601 ggtaaagtgt gtttattttt tcggcgttta attatttaag aagtatttag ttacaataag
      661 tgactagatc ccttttttcg ctcattgaat cctctgtgcc aggcagtaca atcccgcagc
      721 tttgggaatt ttgattaatt gcttgtggga aaggggataa tgaaaaaaat tgcgctagca
      781 ggtctggccg gaatgctgtt ggtttctgca tcggtcaatg caatgagcat cagcggtcag
      841 gcgggtaaag aatacaccaa tattggtgtc ggttttggga ctgaatcgac gggcctggcg
      901 ttaagcggta actggacaca taacgacgac gacggtgacg tcgctggcgt ggggctgggg
      961 ttgaatctgc ctctagggcc gttaatggcg accgttggcg gaaaaggcgt ttacaccaac
     1021 ccgaattacg gcgatgaagg ttatgccgca gcggtaggag gtggtttgca gtggaaaatt
     1081 ggcaacagct tccgtttgtt tggcgagtat tactactctc cggattcact ctccagcggt
     1141 attcaaagtt atgaggaagc gaatgctggc gcgcgttaca ccattatgcg tccagtcagt
     1201 attgaggcgg gttatcgcta cctgaatttg tcgggtaaag atggtaaccg cgacaacgcc
     1261 gtggctgacg gcccgtatgt tggggttaac gccagtttct gatcccttcc ggcgcggcat
     1321 tctgtcgcgc cattcagttt cttactacgc cttatctccc tttgcgttat agtgattcga
     1381 taacaaaagc aggagataac catgttaaaa gtggaaatgt tatccaccgg ggatgaagtg
     1441 ttacacgggc aaatcgttga cactaacgct gcctggctgg ccgatttttt ctttcatcag
     1501 gggctgccat tatctcgcct caatacggtg ggggataacc ttgatgactt agtcaccatt
     1561 cttcgcgaac gtagtcagca cgccgatgtg ctgatcgtta acggcgggct gggaccgacc
     1621 agcgatgatt taagcgcact cgccgctgcg acagcaaaag gtgaaggcct ggtgctgcat
     1681 gaagcctggc tcaaagagat ggaacgctat ttccacgaac gtggacgagt aatggcaccg
     1741 agcaaccgta aacaagcgga gctgcctgcc agtgctgaat ttatcaataa cccggtaggc
     1801 accgcctgtg gttttgccgt gcagcttaat cgttgcctga tgttctttac tcccggcgta
     1861 ccgtcagaat ttaaggtgat ggtcgagcac gaaatcctgc cgcgcctgcg cgagcgtttt
     1921 tctttaccac agccgccggt ttgtctgcgt ttaactacct ttggtcgttc ggaaagcgat
     1981 ctggcacaaa gcctggacac tctacaactg ccgccgggcg taacaatggg ctatcgctct
     2041 tcaatgccga ttatcgaact gaaactcacc ggaccggcaa gcgagcaaca ggcgatggaa
     2101 aaactgtggc tggacgttaa acgtgttgcc ggacagagcg tgattttcga aggcaccgaa
     2161 ggactgcccg tgcagatcag tcgcgaattg caaaaccgcc agttcagcct gacgttgagc
     2221 gagtaattca ccggtggttt attggctttg caactttctc gcgcaggtgc tccattgctg
     2281 gcgtgtgaag tggttccttc acaggaagaa aacctggcgc aaactgcgca ctggattaca
     2341 gaacggcggg ccaaccattt tgccgggctg gcactggctg tttcgggttt cgagaacgaa
     2401 catctcaact ttgcgctagc cacaccagac ggcactttcg ctctgcgtgt gcgtttcagc
     2461 actacgcgct acagcctggc tatccgtcag gaagtgtgcg caatgatggc actgaatatg
     2521 ctgcgccgtt ggttaaacgg ccaggatatc gccagtgagc atggctggat tgaggttatt
     2581 gagtccatga ccttatctgt ctgattccgc cactctgtgc cggtttactg caccggcaca
     2641 ttccccctcc ggcaaaactt gtccaacctg ttgcactcaa taagaaaaaa tccgtgcact
     2701 gattcacaaa tttgtgcgta atttctccag tccgctggac gaggagcggg atttgatgcg
     2761 cttcacaatt ttactccccc tgagcaacga gactgattcc agtatctaag actggagtcc
     2821 agtatgttgg aatcaagcaa agttcccgcc ctgacacggg ctattgatat tctaaatttg
     2881 attgcccgca ttggcccctg tagtgctgcg acgatcattg acacattggg aatccctaaa
     2941 agcacggcct atttgctgct taatgaactc aggcgtcagc gttttcttag ccttgatcac
     3001 caggaaaatt tttgtttgtg gaccaggctg gtggagcttt ctggtcatgc gttaagcaag
     3061 atggatcacc gggaactggc tcgaccgcgt ttgacgcaac tgatggatac caccgggctg
     3121 ttatgtcatc tggggattat tgataacggc agcgcttatt acattttaaa agtggagtca
     3181 tcagccacta tcagcgtgcg ttcccatgaa ggaaaaagcc tttcgcttta tcgctccgga
     3241 ataggtaaat gcctgctttc ctggcaacct gcagcggtac agcaaagtat tatcgaaggg
     3301 ctggtctggg agcaggcgac acccacaacg attactcatc cacagcaact tcacgaagaa
     3361 ctggcgcgta ttcgccgtca gggctggagc tatgacaacg gcgaagatta ccccgatgtc
     3421 cgttgtgtcg ctgcgccggt gtttaacgcc aataacgagc ttaccgctgc gatctcggtg
     3481 gtcggtaccc gcttacaaat caatgaagaa taccgtgatt atctggcggg caagtccatt
     3541 gcctgcgcca gggatatttc acgtctgttg ggatggaaaa gtccctttga cttacaagcc
     3601 tcataacaat ggagaacatc atgaccctac caaaaattaa acaggttcgc gcctggttta
     3661 ctggcggtgc gacagcagaa aaaggcgctg gcggcggtga ttatcacgac cagggggcga
     3721 atcactggat agacgatcat attgccaccc cgatgagtaa ataccgcgat tacgagcagt
     3781 cacgccagtc atttggcatt aacgttcttg gcacgttggt tgttgaagtc gaagcagaaa
     3841 acggccagac cggattcgct gtttcgacag ccggtgaaat gggctgtttt atcgtcgaaa
     3901 aacaccttaa ccgtttcatt gagggtaaat gtgtcagtga tatcaaactg atccacgatc
     3961 aaatgctcaa tgccaccctg tattactccg gctctggtgg cttggtgatg aatacgattt
     4021 cctgtgtcga tctggctctg tgggatctgt tcggcaaagt ggtcgggctt ccggtttata
     4081 aacttttagg cggcgctgtt cgtgatgaga ttcagttcta cgccacaggt gcgcgtccgg
     4141 atctggcaaa agagatgggc tttatcggtg gcaaaatgcc gacgcactgg gggccacatg
     4201 atggcgatgc ggggatccgc aaagatgccg ctatggtcgc ggatatgcgt gaaaaatgcg
     4261 gtgaagattt ctggttaatg ctcgactgct ggatgagtca ggacgtgaac tatgcgacca
     4321 aactagccca cgcttgcgcg ccctataacc tgaaatggat cgaagagtgc ctgccgccac
     4381 agcagtatga aggttatcgc gaactgaaac gcaacgcgcc agtcggaatg atggtcacca
     4441 gcggtgagca ccacggcaca ctgcaatctt ttcgtacgct ttcagaaacc ggtatcgaca
     4501 ttatgcagcc ggatgttggc tggtgcggtg gtttaaccac gctggtggaa attgccgcaa
     4561 tcgccaaatc cagggggcaa ctggtggtgc cgcacggttc gtctgtttac tctcaccatg
     4621 cggtgatcac cttcaccaat acgccattca gcgaattcct gatgaccagc ccggattgtt
     4681 caacgatgcg tccgcagttt gacccgattc tgctcaatga gccggttccg gtgaatggtc
     4741 gtattcataa atcagtgctt gataaacccg gttttggcgt cgaactcaat cgtgactgca
     4801 atttgaaacg cccctacagc cactaatcac tccgggcgtt gctgtgacaa cggtaacgcc
     4861 cacatattct aaggagcttt ttatgagcac cgctttgctt gacgccgtgg tgaagaaaaa
     4921 ccgtgtgcgt ttaattccgt ttatgttggc gctgtatgtg ctggcgtttc tcgaccgttc
     4981 gaatatcggt tttgccaaac agacctacca gattgatacc gggttgagta atgaagctta
     5041 tgcgctggga gcaggcattt tctttgtggt atatgcgttt ctgggggttc cggcgaatct
     5101 tttgatgcgc aaactggggg ccagaacctg gattggtacg acaacactgc tgtggggatt
     5161 tctttcggca gccatggcat gggccgatac tgaagcgaaa tttctgatag ttcgcactct
     5221 gcttggtgct gcggaggccg gatttttccc tggtatgatt tatctcacct cgcaatggtt
     5281 tccgcagcgt aatcgcgcca gcattatggg gctgttctat atgggcgcac cgctggcgtt
     5341 aacactggga tcaccgcttt ccggcgcgct gttggagatg catggattta tggggcatcc
     5401 cggctggttc tggatgtttg tgattgaagg attgttggca gtcggtgctg gggtattcac
     5461 attcttttgg cttgatgaca caccggagca ggcacgtttt ctgagtaaac aagaaaaaac
     5521 gttgcttatc aatcaactgg caagtgaaga acaacagaaa gtgacttctc ggctgagcga
     5581 tgcgctgcgt aatggccgag tctggcaact agcgattatc tacctgacca ttcaggtggc
     5641 ggtttacgga ttaattttct tcctgccgac ccaggttgcg gcattgctgg gaacaaaagt
     5701 gggctttaca gcgtcggtgg tcaccgccat tccgtgggtt gcggccttgt ttgggacctg
     5761 gcttattccg cgctattccg ataaaaccgg cgaacggcgt aatgtcgcag cgctgacatt
     5821 actggcggcg ggcattggta ttggtctgtc cgggctgctt tctccagtaa tggcgatcgt
     5881 agcgctgtgt gttgcagcta tcgggtttat tgccgtgcag ccggtgttct ggacgatgcc
     5941 gacacaactg ctttccggta cggcgctggc tgcggggatt ggttttgtaa acctgtttgg
     6001 tgcagtgggc gggtttattg ccccgatcct gcgcgtgaaa gcagaaacgt tatttgccag
     6061 cgatgcggca ggattactga cactggcagc ggtggcggtc atcggttcgc tgattatttt
     6121 cactctgcgt gtaaatcgta ctgttgcgca gaccgacgtg gcacatcatt aaataggtta
     6181 aggaacacga tgaacgcatt attaaccaat ccctttaaag aacgtttacg caagggcgaa
     6241 gtgcaaattg gtctgtggtt aagctcgact acttcttata tggcagaaat tgccgctact
     6301 tccggttatg actggttgct gattgacggg gagcacgcgc caaacaccat tcaggatctt
     6361 tatcaccagc tacaggcggt agcgccctat gccagccaac ccgtgatccg tccggtggaa
     6421 ggcagtaaat cgctgattaa acaagttctg gatattggcg cgcaaactct attgatcccg
     6481 atggtcgata ctgccgatca ggctcgtcag gtgtcgtctg ccacgcgcta tcctccctac
     6541 ggtgagcgtg gtgttggggc cagtgtggca cgggctgcgc gctggggacg cattgagaat
     6601 tacatggcgc aagtcaacga ttcgctttgt ctgttagtgc aggtggaaag taaaacggca
     6661 ctggataacc tggacgagat cctcgacgtc gaagggattg atggcgtgtt tattggacca
     6721 gcggatcttt ctgcgtcgtt gggctacccg gataacgccg ggcacccgga agtgcagcga
     6781 attattgaaa ccagcattcg gcggatccgt gctgcgggta aagcggctgg ttttctggct
     6841 gtggctcctg atatggcgca gcaatgcctg gcgtggggag cgaactttgt cgctgttggc
     6901 gttgacacga tgctctacag cgatgccctg gatcaacgac tggcgatgtt taaatcaggc
     6961 aaaaatgggc cacgcataaa aggtagttat tgatatcaaa ggcccatggg gatcggctgt
     7021 gggcctgtgt taattagtgg ttattcgctg ccagatcggc ttcgcttagc tgggtggccg
     7081 cgagcacctg gtcacgatca atgccctgtt ccagcatgcg caaagcaatt ttaatggctt
     7141 gttcatgcat accttcctgc cagccaattt gatgcccttc ctgccgtagc cgttcagcaa
     7201 tagtcattaa tctctccttt tgtagtggtg aacgttcggc aatctcctca ataaaacggg
     7261 tgaaacggga ggtatcgccg cattgcagca gataattaaa cagtgtttgt agctggctgt
     7321 cattagtgaa gcctctaacc aaaagcgtgg tgatcctgtc gaccatgccg attaaatcgc
     7381 ggtcgcgaat atgcttttga atcagttcca gcagagctat acgccgatgt tgcatgatct
     7441 catcgtcagg tacgatggtg atatccacca acggaaaagc ttcggtgtac aactgccggg
     7501 caagttgcgg atcgtcaaac tcatccagcc agttaagtga gtacgggtag ggcgatgttt
     7561 cgccatgata aaacagcaat ggcaccacca gcggaactct gtcatagcct ttatccaggt
     7621 gacgctgcat ggcggcagtg gcatagcgca ttagccgaaa agccatattc ttttctgcag
     7681 agctttgatg ttcaatcacg cagtagatat agccgtcacc gtcgctggtt tccacggacc
     7741 acaaaacatc cgagtagtaa gcgcgtaaac ttttttcaat aaaactagtg ggttccaggc
     7801 gtaaggtttg caggttgcaa agcttgcgca gtggttctgg taaatgtatt tcgagaaaat
     7861 cccgtgcggt ttcgggtgtg aacataaagg ttttaaatac cgcatcatgc ggggaggagg
     7921 ttgttgattc ggtcatggtt gtccgtcacc tgatatttat gatgacgcga cattacggtt
     7981 atcctaggcc tttgacatct gccagtttca gcctttgcga gacgttttca gaggaatttt
     8041 cactgcgttg caacgcatac attgggcacg gaatcgtgta cgcaaaaatg cccggtaacc
     8101 acatgcttac cgggcatcag aaaggagttt aagccagcgc ctgggccagt agcgtaatcg
     8161 gatgttcgca gcgaagactt gtggacatct caatctgcca tttacaggtt tcgcagtcgg
     8221 tgaccaccag atctgcgccg ctttcttcta tctggcggaa cagtggtgcg ccgatggctt
     8281 gtgaggtggg gtagttctct tttttgaaac cgtaagtacc cgcaataccg cagcactggg
     8341 aatccagcac cgttaactca agccccggga ttttacgcaa cagctccagg gtgtagagcg
     8401 tccagcccat tttttccata tggcacggag tgtgataaac cactttcagc ggcagcggtt
     8461 tcagcggtaa cgttttgcct tcgtccagct tgcgccacag ccagcgggtt gccagttcga
     8521 tatgatcgcg caagcctttg ttgtcgacat tcagcacttc cgggtattcg tcgcgcaggg
     8581 caaatgtaca ggttgaggag gtggcaatca ccggaatgcc ttttactccc acagcttcgc
     8641 ggatcgactc tacattcgta attgcctgtt tgcgtgcttt atcggtaaag ccgttggcga
     8701 ttagcggtac gccgcagcat ttttctttgc tgagcagttg tacaccggta cccattgcgt
     8761 tgagcacttt aattaaatct ttacctaact gcggatggtt gtagttaacg aagcagccgt
     8821 gaaagaaagc gacctggtct ttatattgtg cttgctgagc cgctatgctg cgataccagc
     8881 gacggaacgt gccgaaggag tatttcggta gcgtgcgacg atgatcgatt tttaacgccg
     8941 catcaagcag ctgccgcacc ggtttcagcg aggtagcggt gttgacgatt ggtgcgaacg
     9001 gcgtggaaac gctacccatc aggtcggtat gactcaacac aaaattacgc agcgacgggc
     9061 gcgtggtgtc atattttgcc cgcgcgcgct ggataatatc gccaatcttc acatcggacg
     9121 ggcaggcgac ttcacaacgt ttgcagttga tgcaatattt cagcgcctcg tcatacagtg
     9181 cgccatcttt caaacgcaga cgctcgccat ccggcccggc ttgttttggc cctggataac
     9241 cgggattcac ccggctcacc gggcaggcgg tggtgcagac ggtgcactta atgcagtttt
     9301 cgaagctggt gtcgttcatt gttggcctcc tgcgcgttgg gcaatctgtt gagcggcatg
     9361 taaagcactg acggcacaaa caccgccgcc gcatccctgg gcgatgggat caaatccgcc
     9421 cagcaccgaa ccgatggcaa acaggttctc aatggtttgc cctgcctgcg acgggcgtag
     9481 cgtctcatca gtggttacac cgaactgctg ccacggttgc ggcgcaaaaa aatcgccctt
     9541 ataccattca ccccgcgtgg cggtttgtag cacatcaagg ccgagaatcg gctcgcgaat
     9601 gccgttacgt tccgctacca gtccgccact aaagaaactg ccgctggcga gaaccgcgaa
     9661 atgtggacgt agcggaatat cggcgtgatt gcgggtccag atttcattca ctacgccatt
     9721 tttacaggtc acttttttca cttcatcgcc cggcatccac acgccaccct ggcgcacaaa
     9781 ctggcgctgt aactggtttt gcagacgaat gcccagcacg gaaggcggca gcgttggcaa
     9841 aagcatcagt gaacaaagta gtttttcatt caaccaacgc cacagtttgt catcggccag
     9901 gccgaagcag gcaggcatca ggatcatttc gcaggtattg gcgacaggaa taagcgcatc
     9961 aagtaacagc ggccagtttt cttcattatc aaggaaacgg gcgatattca cagcgcgaaa
    10021 ttcggtggcg ttattgcgca ggacatccag ttccggcagc tctatttctg cggtttcaac
    10081 cgaaagatcc agttcgtgca acgaagccgc cgcaagatgc gcctgaaaat ccatcaggcc
    10141 gcttatcccg accacacata ttttcttcgc gggcaacggc cagacgggga cttctggcga
    10201 acttagccag gtagagcgca gagtgccgag cggcgtaatc cgctggtgag ccagttctac
    10261 gctgccctgc aattgcgcac cgctttcagc gattaatgcc tgcgcctggc aagcgagatc
    10321 gagcacgcgt tgtggcccga gaagagagta aggatgtgca ggtgcctgct gacgcaaaga
    10381 ttccagtcca ctgtgaatgt ctgccacggg ttgaccatct ggcagatggc tcagcaaatc
    10441 cagcgaaccg gatgagaaat gcaacgcgct ttgaccacgc gtgacaatgg cacagcgcag
    10501 gccgtgtttt tgcagttgca ggccacagag taatccggcg aggccgccgc ccataatgac
    10561 agtatcaaag cgcatctttc tgctccttct ccagaccaca cagtccctga taaacccagc
    10621 gggtaaattc gctttcgcgc agtgcgtctc cccaggcgat gggttgcacg cctttccagc
    10681 gttcgttaag gaaggtggaa agttgctcga tagattgcgc ggacgtcgtg acgttaaacc
    10741 gttgcagcag tccggcagcg cggcaggcgc agagttcgcc ctggcaggtg cccatcccca
    10801 cacgggtacg acggcgtaaa tccagcaggc tattaacgtt taaattttct accgcgtact
    10861 gcacttcacc cgcagttacc gcttcgcact cacataccag gctacggtgc agacggcctt
    10921 cgctcagcca ggcaggcgtg cgatcgccat gacgataaac cgcagaaccg cgcagcgggg
    10981 caggcaggga gatgacttta cgcaaggtaa cttcagcggg ttcttgtgaa ccaggcagtg
    11041 ccagatcggc agtcgtacag gggcgcgtgt tgcccagttt gcggcatacc gcgtcggtag
    11101 cccattcagc catcagccga taggtcatca gtttgccacc ggtgatggtg ataaatccgt
    11161 ccagaccatc gcgttcagca tggtcgagca gcacgatgcc acggctgacg ttacgtccgc
    11221 tcggatcgtc atcgctggca accagcgggc gcacgccaga ataggcccgc aaaatgcgcg
    11281 ttttcgccat cacgggggcc agtttttccc cttcacgcag cagaatatca acctcttctg
    11341 ccgtcactcg attatcgtca atctcgttgt aatcaatacg taaagaggtg gtaccaatca
    11401 gcgaaatggt atcgccaggc accagaatat cggcgtcgga aggtttacgg cagcggttga
    11461 tcacatgctg gttaatgcgg tgatccatga tcagcagcga tcctttcgcc gggaacatgc
    11521 gaatgcgcag atcggcatat tcggcaatgt gttgccccca gatcccagcg gcattaacca
    11581 cgacaggtgc atgaagggcc tgagtttcgc cggtgagatg gttacgtaca cgaacaccgc
    11641 acaccgtcgc gccttcacga atcagccccg tgacttcatg agcggtaagg ataacggcac
    11701 cgtgttcttt ggcatccagc atgtttgctg cggtcagacg aaatggatca acggtgccat
    11761 ccggaacttt caccgcgcca atcagtgccg ggttaacggc aggttcgata atgcgcgctt
    11821 gctgcgggtc tatagcttct gcgctgatcc ctgcttcttc gcaggcgcga ataaaagtgg
    11881 cctggaatga gaggtcatct tccggcaggg tgataaacag gccgttggtt ggttcaacgc
    11941 agtgacgtgc aatgcgtttc aggatctggt tttcactaat gcattcgcgg gccgattccg
    12001 catcggttac cgcatagcgc gcaccgctgt gcagcaggcc gtggttacgc ccggtggcac
    12061 cggttgcgat gtcgtggcgc tcaaccaaaa tcacgcgcag cccgcgcagg gcacagtcgc
    12121 gggcaatccc ggctcccgtt gcgccgccgc caatgataat cacgtcactt gattgcgagt
    12181 cgcgagtttt cattgttatc cctctgaagt tcgtttttta ccatttagcc atagtaaaaa
    12241 catgaattgt ttgatttcgc gcatgttcgc tcataattcg aaagtgaaac gtgatttcat
    12301 gcgtcatttt gaacattttg taaatcttat ttaataatgt gtgcggcaat tcacatttaa
    12361 tttatgaatg ttttcttaac atcgcggcaa ctcaagaaac ggcaggttct ctcactgaat
    12421 caggctgtta atcataaata agaccacggg ccacggaggc tatcaatgtt gagtattttt
    12481 aaaccagcgc cacacaaagc gcgcttacct gctgcggaga tcgatccgac ttatcgtcga
    12541 ttgcgctggc aaattttctt ggggatattc tttggctatg cggcttacta tttggttcgt
    12601 aagaactttg cgcttgctat gccttatctg gtcgagcagg gattctctcg cggtgattta
    12661 ggtttcgcgc tttcggggat ctcgatcgct tatggatttt cgaaattcat aatgggttcg
    12721 gtatcagatc gctcgaatcc gcgcgttttc ctccccgcag gtttgattct ggcggcggca
    12781 gtgatgttgt ttatgggctt tgtgccatgg gcgacgtcga gcattgcggt gatgtttgta
    12841 cttttgttcc tctgcggttg gttccagggg atggggtggc cgccgtgtgg tcgtactatg
    12901 gtgcactggt ggtcgcagaa agaacgtggc ggcattgtgt cagtgtggaa ctgtgcgcac
    12961 aacgttggtg gtggtattcc gccgctgctg ttcctgctgg ggatggcctg gttcaatgac
    13021 tggcatgcgg cgctctatat gcctgctttc tgcgccattc tggtggcatt attcgccttt
    13081 gcgatgatgc gcgatacccc gcaatcctgt ggcttgccgc cgatcgaaga gtacaaaaat
    13141 gattatccgg acgactataa cgaaaaagcg gaacaggagc tgacggcgaa gcaaatcttc
    13201 atgcagtacg tactgccgaa caaactgctg tggtatatcg ccatcgccaa cgtgttcgtt
    13261 tatctgctgc gttacggcat cctcgactgg tcaccgactt atctgaaaga ggttaagcat
    13321 ttcgcgctgg ataaatcctc ctgggcctac ttcctttatg aatatgcagg tattccgggc
    13381 actctgctgt gcggctggat gtcggataaa gtcttccgtg gcaaccgtgg ggcaaccggc
    13441 gttttcttta tgacactggt gaccatcgcg actatcgttt actggatgaa cccggcaggt
    13501 aacccaaccg tcgatatgat ttgtatgatt gttatcggct tcctgatcta cggtcctgtg
    13561 atgctgatcg gtctgcatgc gctggaactg gcaccgaaaa aagcggcagg tacggcagcg
    13621 ggctttaccg ggctgtttgg ttacctgggc ggttcggtgg cggcgagcgc gattgttggc
    13681 tacaccgtgg acttcttcgg ctgggatggc ggctttatgg taatgattgg cggcagcatt
    13741 ctggcggtta tcttgttgat tgttgtgatg attggcgaaa aacgtcgcca tgaacaatta
    13801 ctacaaaaac gcaacggagg ctaatggcat gaaattgacg ctgaaaaacc ttagcatggc
    13861 gatcatgatg agcactatag tcatgggaag cagtgcaatg gcggcggaca gcaacgaaaa
    13921 aatagtcatc gcccatcgcg gtgccagtgg atatttgccg gagcatacgc tgccagcaaa
    13981 agcgatggcg tatgcgcagg gagcggatta tctggaacag gatttggtga tgaccaaaga
    14041 cgaccatctg gttgttctgc atgaccatta tctcgatcgt gttactgatg ttgccgatcg
    14101 tttcccggat cgggcgcgca aagacggtcg ttactacgcg atagatttca cgctggatga
    14161 aattaagtcg ttgaaattta ccgaaggttt cgatattgaa aacggtaaaa aagtgcagac
    14221 ttatccgggg cgtttcccaa tgggtaagtc cgacttccgg gtgcacacct ttgaagaaga
    14281 gattgaattt gttcaggggt taaatcactc taccgggaaa aatatcggta tttatccaga
    14341 aatcaaagcg ccgtggttcc atcatcagga agggaaggat attgcggcaa aaacgctgga
    14401 agtgctgaag aaatatggtt acaccggtaa agacgataaa gtttatttgc aatgttttga
    14461 tgctgatgag ctgaagcgta ttaagaatga gctggaaccc aaaatgggca tggagctcaa
    14521 tctggtacag ctgattgcct ataccgactg gaatgaaacg cagcagaaac agccggatgg
    14581 aagctgggtt aattacaact acgactggat gtttaagccg ggtgccatga aacaggtggc
    14641 ggaatatgca gatggtattg gtccggatta ccatatgttg attgaggaga catcgcagcc
    14701 gggtaatatc aaactcactg gcatggtgca agatgctcag cagaataaac tggtagtgca
    14761 tccttatacc gtgcggtcag ataaactgcc tgaatacact cctgatgtga atcagttata
    14821 tgatgctctg tataacaaag cgggtgtaaa tgggctgttt actgatttcc ctgataaggc
    14881 agtaaaattt cttaataaag agtaatgtta ctgcaggatg gcgttcatgc gccatcctgt
    14941 ctcatatcaa atttttccaa tcgccactaa ttgttccatg cacatttgta gtaaaaacag
    15001 tggactgcct tctaccggct tttctttagc cagcaacgaa atctgtcgat agaaagttgg
    15061 ttcgagagga acggaacaca attcacctgc aatgcttttc agcgttaact ctggttgtaa
    15121 ggcgatgcct aatccctgtc gaataaaatt catcgctgta tccgcctgat taaattcaca
    15181 ttttatctgc tgggcgcact attagtggta gtggctggtg gaatctatgc gctgcaaatg
    15241 aagggtaaaa gcggtgtcaa gctagtagtg gcaaaagaaa ttcattaata cgacacgttt
    15301 cattaagatt ttcctcaggt aaccacctat agtcattgtc gttaataaaa acgacaggcg
    15361 atagggtatg gcagtttcag caaagtacga cgaatttaat cactggtggg caacagaggg
    15421 cgactgggtt gaagagccta actatcgccg taatggtatg agcggggtgc aatgcgtcga
    15481 gcgcaacggc aaaaagctgt atgtaaagcg catgacgcat catctgtttc attctgtacg
    15541 ttatccgttc ggccgaccaa cgattgtccg tgaggttgcg gtaattaaat aactggaacg
    15601 ggccggtgtc atcgtgccga agatcgtttt tggtgaagcg gtgaaaattg agggtgaatg
    15661 gcgagcgttg ctggtgactg aagatatggc ggggttcatc agcattgctg actggtatgc
    15721 ccagcatgca gtatcgcctt attctgacga agtacggcaa gccatgttga aagcagtggc
    15781 gctggcgttt aagaaaatgc atagcattaa tcgtcagcat ggctgttgtt atgttcgcca
    15841 tatctatgtg aaaacagaag gtaaggcaga agctggtttt cttgacctgg agaaaagtcg
    15901 tcgccgcttg cgtcgcgata aagcgattaa tcatgacttc cgccagctgg aaaaatatct
    15961 ggagccaatc cccaaagcgg actgggagca ggtgaaagcg tattactacg ccatgtaatc
    16021 gttgtaggcc agataagaca cataagcgtt gtatctggca ctacaaccaa ttcacatctc
    16081 gatttcaata tcgccttttg cccggcaaca acagggcaaa atttcccccg gctgaataaa
    16141 ggctaacggt tcggcaatcc agtcaacttg acctgcaacc aggcgtgtgc gacaggagcc
    16201 gcagtaacct tcgcgacact ggtactcaac cgccacattg tgggattcca gcgctgccag
    16261 aagggaaggg tgttcatcct ggcacagcag ttgtgtgcca gtgatgcgca gggtaacgcg
    16321 ggccatcaga gctggaagtt actcaaatcg tcggtgtcca cttccgagtc aatctgcccg
    16381 accagataag aactgacttc cacttcctgc ggagcaacct gcacgttatc agacaccagc
    16441 caagtgttga tccacgggat cgggttggag cgcgtctgga acggcagatc caaaccgact
    16501 gcctgcatac ggatattggt gatgtattca acgtactggc agagaatgtc tttattcaga
    16561 ccaatcatcg aaccgtcgcg gaacagataa tccgcccagt ctttctcctg ttgagctgcc
    16621 tgaacaaaca ggtcatagca ctcctgctta cactcttcgg caatttccgc catctcagga
    16681 tcgtccgcgc cgctgcgcag cagattcagc atatgctggg tgccggtcag gtgcagggct
    16741 tcgtcgcggg caatcaggcg aataattttg gcgttgcctt ccatcaattc gcgttctgca
    16801 aaggcaaagg aacaagcaaa gctgacgtag aagcggatcg cttccagcgc gttaacgctc
    16861 atcaggcaaa gatacagttt tttcttcagc tcgcgcaggc taacggttac ggttttaccg
    16921 ttaacggtgt gggtgccttc gcccagaagg tgccagtagc tggtcatttc gatcagttca
    16981 tcgtaatagc tggagatccc ttccgcacgt ttctggatct gctcgttggt gacgatatcg
    17041 tcaaacacaa cagacggatc gttaacgata ttacgaatga tatgagtata ggaacgggaa
    17101 tgaatcgttt ctgagaacgc ccaggtttcg acccaggttt ccagttccgg aatagaaata
    17161 agcggcaata gcgccacgtt cggactacga ccctgaatgg aatccagcag cgtctgatat
    17221 ttcaggttgc tgataaaaat gtgtttttcg tgctccggca gcgcctggta atcgatacgg
    17281 tcgcgggaga cgtcaacttc ttccggacgc cagaagaaag agagctgctt ttcgatcagc
    17341 ttttcgaaga tgtcatattt ttgctgatcg tagcgggcca cgttgaccgg ctgaccaaag
    17401 aacatcggtt ctttgagctg atcatttttc gtctgtgaaa aggtggtata tgccatgagt
    17461 gtgtcctgtt gggagtttaa tgccggataa ggcattttac gccgcatcct gtgcctgatg
    17521 cgacgctggc gcgtcttatc aggcctacaa atccgagccg taggccggat aaggcgttca
    17581 cgctgcatcc ggcatctcaa tatcagatct tacatgcgcc gctttcgcag ccatcgtcct
    17641 ggattgacgg taccagatcg tcctgcgcgt cttcagcgcc gtcacgggtg ttctgataat
    17701 acagcgtttt gaccccgaat ttgtaggcgg tgagcaggtc tttcagcaac tgctgcatcg
    17761 gcacttttcc tgacgggaaa cgtgacggat cgtagttggt gttagcagag atcgactgat
    17821 cgataaattt ctgcatgata cctaccagtt gcaggtagcc atcgttaccc ggcatttccc
    17881 acagcagctc ataggcgtcg tgcaggtgct cgtagtccgg caccacctgg cgcaaaatac
    17941 cgtctttcga cgctttgatg ctgacgtaac cgcgcggcgg ttcaataccg ttagtggcgt
    18001 tagagatctg cgaagaggtc tcggacggca tcagggcaga aagcgtggag ttacgcaggc
    18061 cgtgcgtttt gattgattca cgcaatgctt cccagtcgta atgcagcggc tcattagcga
    18121 tggcatccag atctttctta taggtatcga tcggcaggat ccctttcgcg taagtggttt
    18181 cgttaaacca cgggcactcg ccttgctctt tagccagctc attagacgct ttcagcaggt
    18241 aatactgaat cgcttcgaag gttttatgcg tcaggttgtt ggcgctgccg tcggagtagc
    18301 gtttaccgtg cttcgccagg tagtaagcga agttgatcac accaataccc agcgtacgac
    18361 gacccatcgc tccacgtttg gcggccggga tcgggtaatc ctgataatcc agcagcgcgt
    18421 caagtgcacg aaccgccaga attgccagct cttccagttc atccaggtta ttaattgcgc
    18481 ctaggttgaa agcagacagc gtacacagcg cgatttcacc gttctcgtcg ttgacgtcgt
    18541 tcagcggttt ggtcggcagg gctatctcca ggcacaggtt agactgacgc actggcgcga
    18601 tggccggatc aaacgggcta tgggtattac agtggtcaac gttctgaata tagatacgac
    18661 cggtagacgc acgttcctgc atcatcagcg agaacagctc aacggctttc acacgctgct
    18721 tgcggatgct gtcgtctttc tcatatttgg tatacagacg ttcaaactct tcctgatcgg
    18781 cgaagaacgc gtcgtacagc cccggtacgt cggacgggct gaacagggtg atatcttcac
    18841 ctttcagcag acgggtatac atcagtttgt tgatttgtac cccgtagtcc atatgacgca
    18901 cgcggttgcc ttccacacca cggttgtttt tcaacaccag caggctttcc acttccagat
    18961 gccacatcgg gtagaacagc gttgccgcac cgccgcgcac accgccctga gagcaggatt
    19021 tcaccgctgt ctggaaatgt ttgtagaacg gaatgcagcc ggtatggaac gcttcaccac
    19081 cgcgaatcgg gctacccagc gcacgaatac gcccggcgtt gatgccgatc ccggcacgct
    19141 gggaaacgta tttaacaatc gcgctggagg tggcgttgat ggaatccagg ctgtcaccgc
    19201 actcgatcag tacgcaggag ctgaactgac gagtcggggt acgcacgccg gacatgattg
    19261 gcgtcggcag cgaaatttta aatgtggaaa ccgcgtcgta aaaacgcttc acatattgca
    19321 ggcgcgtttc acgcgggtag ttcgagaaca ggcacgcggc aaccagaata taaaggaact
    19381 gggcgctctc atagatttcg ccggtcacgc ggttctgtac cagatatttg ccttccagct
    19441 gcttaacggc agcataagag aaggtcatat cacggtcgtg atcgataaac gtgtccatct
    19501 gcttgaactc ttcttccgtg tagtcttcca gcagatgatt atcgtatttg cccatctcga
    19561 ccattttcac cacgtggtcg tacagcgcag gcggctcaaa ctggccgtag gcttttttac
    19621 gcaggtggaa gatcgccagg cgcgcggcga gatactgata atccggcgca tcacgggaga
    19681 tcaggtctgc ggcagccttg ataatggttt cgtggatgtc agaggtcttg ataccgtcat
    19741 aaaactgaat gtgggagcgc agctcgacct gggaaatcga aacgttatgc agtccttctg
    19801 ccgcccaatc cagaacgcga tggattttgt cgagattgat gcgctctgtg ctaccgtcgc
    19861 gctttgtcac cagcagattc tgattcatgt atgtcgtacc tgtttttgga attctttccg
    19921 caatagtttc atgcagacta cctgtagtac gggaagctgc ttagaacact atatataggg
    19981 ggtatgtttg ggattcactg caagatagtg tgaaaatggc cctcttgcaa gtgcataact
    20041 ttgtggataa ctcaggaagg aaaaagtggc tttcgcgcac cttaggtcag acaaggtgtc
    20101 cgggaaagtc aatgggaaga gaaaaatttg ttaaaaataa ctgttcggcg atttcttgaa
    20161 cggcagaata ggttggataa attgatatag atggtttatt aatctttttg ctaatgattg
    20221 caaaaattga tctaagcacg agtcatcaca taatctgatg tatcagttgt ggtcaatata
    20281 taagcgaatg taagcgattg ttgatgtcga aatggttgtt ttctattcgg ttattgttaa
    20341 atgcgaagcc a
//
LOCUS       DAZPEB010000056        20113 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZPEB010000056 DAZPEB010000000
VERSION     DAZPEB010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20113
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.057"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..1985
                     /locus_tag="ACHURJ_001804"
     CDS             <1..1985
                     /locus_tag="ACHURJ_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HFR1280777.1"
                     /translation="VGAFLAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDT
                     FNTEQVEVIKGPSGTDYGHSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVN
                     QVIGDTTAVRLNVMGEKTHDAGRDKVKNERYGVAPSVAFGLGTANRLYLNYLHVTQHN
                     TPDGGIPTIGLPGYSAPSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDI
                     NDNTTIRNTTRWSRVKQDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILT
                     NQTNLTSTFYTSSIGHDVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTR
                     NGANANGQTDTFAIYAFDTLQITRDSELNGGIRLDNYHTEYDSATACGGSGRGAITCP
                     AGVAKGSPVTTVDTAKSGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQS
                     GSGNSANRTDFKPQKANTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQY
                     GKKRVEGYEISVAGNITPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLW
                     SQYQATDDISVGAGARYIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLN
                     VYNLFDTDYVASINKSGYRYHPGKPRTFLLTANMHF"
     gene            2027..2704
                     /gene="ybiX"
                     /locus_tag="ACHURJ_001805"
     CDS             2027..2704
                     /gene="ybiX"
                     /locus_tag="ACHURJ_001805"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFR1280778.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            2778..3044
                     /gene="ybiI"
                     /locus_tag="ACHURJ_001806"
     CDS             2778..3044
                     /gene="ybiI"
                     /locus_tag="ACHURJ_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFR1280779.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            3309..3569
                     /gene="ybiJ"
                     /locus_tag="ACHURJ_001807"
     CDS             3309..3569
                     /gene="ybiJ"
                     /locus_tag="ACHURJ_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFR1280780.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLTEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(3672..3731)
                     /gene="ybiE"
                     /locus_tag="ACHURJ_001808"
     CDS             complement(3672..3731)
                     /gene="ybiE"
                     /locus_tag="ACHURJ_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFR1280781.1"
                     /translation="MRLSANRQGNTCAGRSVTA"
     gene            complement(3797..4882)
                     /gene="hcxB"
                     /locus_tag="ACHURJ_001809"
     CDS             complement(3797..4882)
                     /gene="hcxB"
                     /locus_tag="ACHURJ_001809"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFR1280782.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(5023..5985)
                     /gene="ybiB"
                     /locus_tag="ACHURJ_001810"
     CDS             complement(5023..5985)
                     /gene="ybiB"
                     /locus_tag="ACHURJ_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFR1280783.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLEATGEAATISDGLARVNQAF"
     gene            complement(6013..8163)
                     /gene="dinG"
                     /locus_tag="ACHURJ_001811"
     CDS             complement(6013..8163)
                     /gene="dinG"
                     /locus_tag="ACHURJ_001811"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFR1280784.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGRHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            8283..8765
                     /gene="ybiA"
                     /locus_tag="ACHURJ_001812"
     CDS             8283..8765
                     /gene="ybiA"
                     /locus_tag="ACHURJ_001812"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HFR1280785.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRALLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            complement(8997..10361)
                     /gene="rhlE"
                     /locus_tag="ACHURJ_001813"
     CDS             complement(8997..10361)
                     /gene="rhlE"
                     /locus_tag="ACHURJ_001813"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFR1280786.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSSDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            10590..11261
                     /gene="cecR"
                     /locus_tag="ACHURJ_001814"
     CDS             10590..11261
                     /gene="cecR"
                     /locus_tag="ACHURJ_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFR1280787.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNTTTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            11264..12259
                     /gene="hlyD"
                     /locus_tag="ACHURJ_001815"
     CDS             11264..12259
                     /gene="hlyD"
                     /locus_tag="ACHURJ_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFR1280788.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLKLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQVKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            12252..13988
                     /locus_tag="ACHURJ_001816"
     CDS             12252..13988
                     /locus_tag="ACHURJ_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR1280789.1"
                     /translation="MNDAVITLNGLEKRFPGMGKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATMIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELASEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            13981..15114
                     /locus_tag="ACHURJ_001817"
     CDS             13981..15114
                     /locus_tag="ACHURJ_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1280790.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            15125..16231
                     /locus_tag="ACHURJ_001818"
     CDS             15125..16231
                     /locus_tag="ACHURJ_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1280791.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFTRASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIATNYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(16193..16603)
                     /locus_tag="ACHURJ_001819"
     CDS             complement(16193..16603)
                     /locus_tag="ACHURJ_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFR1280792.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            16736..17487
                     /locus_tag="ACHURJ_001820"
                     /pseudo
     CDS             16736..17487
                     /locus_tag="ACHURJ_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
     gene            17484..18725
                     /gene="clsB"
                     /locus_tag="ACHURJ_001821"
     CDS             17484..18725
                     /gene="clsB"
                     /locus_tag="ACHURJ_001821"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFR1280793.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNKLTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWHPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            18764..19681
                     /locus_tag="ACHURJ_001822"
     CDS             18764..19681
                     /locus_tag="ACHURJ_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFR1280794.1"
                     /translation="MLTWLFFIAVIVLLVVYAKKVDWEEVWKVIRDYNRVALLSAVGL
                     VVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTWVGGIGMRYRLYSR
                     LGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVAQTTLRILGIGLLM
                     IIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALTQMLISSVNWMVMGAIIWLLLGQSV
                     NYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGSIIAALLAYRVLYY
                     FIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(19717..>20113)
                     /locus_tag="ACHURJ_001823"
     CDS             complement(19717..>20113)
                     /locus_tag="ACHURJ_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HFR1280795.1"
                     /translation="IIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVII
                     VNAALMSERPIWVISCLVIVLWPGIISHGRNKLLELAGKCHSEELWSPVRCAFTGALT
                     LYYYFIGFFGILAAIAITLVWQRHTRFFH"
BASE COUNT         4724 a         5446 c         5301 g         4642 t
ORIGIN      
        1 gcgtgggtgc gtttttggcg ggtgagaacg gtaactccac cactggcgac gccatttata
       61 tgcgtggtgc cgatacctct aacagtattt atattgatgg cattcgcgat atcggcagcg
      121 tctcgcgcga caccttcaat accgagcagg tcgaagtgat taaagggccg tccggcaccg
      181 actacgggca cagcgcaccg acgggctcga tcaatatgat cagcaaacag ccgcgcaatg
      241 attccggcat tgacgcctcc gccagtattg gcagcgcctg gttccgccgc ggcacgctgg
      301 acgtcaatca ggtcattggt gataccaccg cggtgcgcct gaatgtaatg ggcgaaaaaa
      361 cgcacgatgc cggacgcgac aaagtcaaaa atgagcgtta cggcgtcgcc ccttctgtcg
      421 cttttggcct tggtacagcg aatcgtttgt atcttaatta tctgcatgtc acccagcaca
      481 acacgccaga cggcggcatt ccgaccatcg gtttgccggg ctattctgcc ccatctgcgg
      541 gaacggcggc cctgaatcat tccggaaaag ttgatactca taacttttac ggcacggatt
      601 ccgattacga cgattcgacc accgacaccg ccaccatgcg ttttgagcac gacatcaacg
      661 ataacaccac cattcgcaat actacccgtt ggtcgcgcgt aaagcaggat tacctgatga
      721 cggcgattat gggcggggcg tcgaatatta ctcagcccac cagcgatgtg aatagctgga
      781 cctggtcacg cacggcgaat accaaagatg tgagtaataa aattctcacc aaccagacca
      841 acctgacctc gacgttctat accagttcta tcggtcatga tgtcagtacc ggcgtggaat
      901 ttacccgtga aacgcagact aactacggcg ttaatccggt gacgttaccc gcggtaaata
      961 tttatcatcc tgacagcagc attcatcccg gcggcctgac gcgcaacggc gcaaacgcca
     1021 atggtcagac ggataccttc gcaatttacg cctttgatac gctgcaaatc acccgtgatt
     1081 ctgagctgaa cggcgggatc cgtctggata attatcatac tgaatatgac agtgccaccg
     1141 cctgcggcgg cagcggacgc ggtgccatca cctgcccagc tggtgtggca aaaggttctc
     1201 cggtcaccac cgtcgacacc gccaagtcgg gcaatctggt gaactggaaa gccggggcgc
     1261 tgtatcacct gacggaaaac ggtaatgtct atattaacta tgccgtttcc cagcagcctc
     1321 cgggcggcaa caacttcgcc cttgcgcagt ctggcagcgg taacagtgcc aaccgcacag
     1381 attttaaacc gcaaaaagcc aacaccagcg agattggcac caaatggcag gttctggata
     1441 aacgcctgtt gctcaccgcc gcactgttcc gtactgatat cgaaaatgaa gttgagcaaa
     1501 atgatgacgg aacttactcg caatacggta agaaacgtgt cgaaggctat gagatatccg
     1561 tggccgggaa tatcactccc gcgtggcagg tgattggcgg ctatacccag caaaaagcaa
     1621 ccatcaaaaa cggcaaagat gttgcccagg atggttcctc atcgctgccg tataccccgg
     1681 agcacgcctt caccttatgg agccagtatc aggcaaccga tgatatctct gttggcgcgg
     1741 gcgcacgcta tatcggcagt atgcataaag gttcagacgg cgcggtggga acgccagcgt
     1801 ttaccgaagg ttactgggtc gccgatgcca aactggggta tcgggttaat cgcaatctcg
     1861 acttccagct aaacgtttac aacctgtttg ataccgatta cgtcgcctca atcaacaaga
     1921 gcggctaccg ttatcacccg ggcaagccaa gaaccttctt gctcacagcc aatatgcatt
     1981 tctgattcag atgtggggcg caggccccac tttttggaga aattgtatga tgtaccacat
     2041 tcccggcgtg ttatcgccac aggacgtcgc tcgttttcgc gaacaactgg aacaagccga
     2101 atgggtggat ggacgcgtca ccaccggcgc acaaggtgcg caagttaaga acaatcaaca
     2161 ggtagacacc cgcagcacgt tatacgccgc cctgcaaaat gaggtgctga acgcggttaa
     2221 ccaacatgct ttattctttg ccgcggcctt gccgcgtacc ctttccacgc cgctgtttaa
     2281 tcgctatcag aacaatgaaa cctatggttt tcatgtggat ggcgcagtac gcagccatcc
     2341 acaaaacggc tggatgcgca ctgacctttc tgccacgctg tttttaagcg atccacaaag
     2401 ctacgacggc ggcgaactgg tcgttaatga caccttcgga caacatcggg taaaactccc
     2461 ggcaggtgat ctcgtgttgt atccctccag cagcctgcat tgcgtgacac ccgtaacccg
     2521 cggcgtacga gtggcatcat ttatgtggat ccagtcgatg atccgcgatg ataaaaagcg
     2581 cgccatgctg tttgaactag acaaaaatat tcagtcgctg aaaagccgct atggtgaaaa
     2641 tgaagagatc ctgtcgctgc tcaatcttta tcataatctg ctgcgggaat ggtcggagat
     2701 ctgacgcttg aactgcccag gttcgtccct atattagcta cacttaactg tacaagcatt
     2761 gatatgggga ggacgatatg gcatccggtt gggctaacga tgacgccgtc aacgaacaga
     2821 tcaacagtac aattgaagat gcgattgccc gcgctcgggg tgaaattccg cgtggcgaaa
     2881 gcctgtatga atgtgaagag tgcggtgccc ccatcccgca agctcgtcgg gaagccattc
     2941 ctggcgtgcg cttatgtatt cattgtcagc aggagaaaga tttacaaaaa ccagcgtata
     3001 caggatataa tcgcagaggt tcgaaagaca gccagttacg ttaactacaa ctggcgagat
     3061 gcatagcgag tataatttgt attttgcgtg gcattccgtg cctttaacgc tgctgtgact
     3121 gccacgcggc ataaaacaag tccataagta caacaaataa atggtttatc agtatgttag
     3181 atgtcaatca atttatttga acaaggcggt caattctctt cgattttatc tctcgaaaaa
     3241 aaacgtgata ctcatcacat cgacgaaaca acgtcactta tacaaaaatc acctgcgaga
     3301 gattaattat gaaaactatc aatactgttg ttgctgctat ggctctttca actctatcat
     3361 ttggcgtgtt cgcggcggaa ccggtaacgg catcccaggc acagaacatg aataaaatcg
     3421 gcgtggtttc tgccgatggc gcatccaccc tcgatgccct ggaagcgaaa ctgactgaga
     3481 aagccgcggc agccggtgcc agtggataca gcatcacttc tgccaccaac aacaataaat
     3541 taagcggtac tgcggtaatt tataagtaat tcgtaggacg gataaggcgt ttacgccgca
     3601 tccggcaaca gatgcctgat gcgacgctac cgcgtcttat caggcctaca aaatccgaac
     3661 cgttggacgg atcaggcggt tacgctacgt ccagcacaag tatttccctg tctgtttgcc
     3721 gacagacgca tatgctctaa ccctcattga tcctatatta cccttgtttg cccgtccgtt
     3781 actggacggg ctttttttag ctggctaact gctgacagaa agcctgcaac gtctcttccg
     3841 acataccaat ctgccgcgcc gcatcacaaa tggcctgcca gcttcccgca tccaatggaa
     3901 tcccctgctc ctgccgttcg cgacgcgtgt tcacttccca ctcgcccggt agcaaaatcg
     3961 gcttatcgtc atcatgcggc gaggctttca cccactcggc aaaggcttca gtctgcgcgc
     4021 tacaatccgg cgcgccgaac agttccgggt tgatgatgat agtggtcatg cagttaagaa
     4081 tggcatcggg actggtttgt aacgtttcct gatgcgtcgt tttaccgcca gaaagcgccc
     4141 cgccaagaat ttcacacatc gctgcaaggg cgtagccttt atgttcggca aaggtcaaca
     4201 gcgaccccaa cggcgactcc tgcattaccg ccggattggt cgtcggcacg ccgttaacgt
     4261 caatcaggca acctggcggc acggggacgc ctttatgcca ggcgacgcga gttttaccaa
     4321 atgcgatggc gctggtggcg taatcaagca acagcgggaa attatcttta cgagggaaaa
     4381 ccacacagaa cggattggtg ccaaagcggc tgtcgcgacc gtggaacggt gcgaccattg
     4441 gaataccgac cacgctaaca aagtggatag agacaaaccc cgccgctgca cactgctccg
     4501 cccagtaacc gatacggccg atatgatgcg agttatgtag cgccacggca gcaataccgt
     4561 gctgatgcgc tttctcaatc cccagcgcca tcgcttcgtg tgccgcgacc tgaccgaatg
     4621 cgcgatcgcc atcgagcgtg actgccgccc ccgcctcttt aacgattttg gcatgatggt
     4681 taatttgcag atgcccctga ctccaggaac gcacatagct tgggatcatg ccaataccat
     4741 gagaatcatg ccctgccagg tttgccgcga ttaaatgatc ggccactaat ttcgcttctt
     4801 gttcctcgct acccatctga cgaaatacag cctgaataaa actgtgcagc gtctgagcat
     4861 caaagcgatg accactttcc atggttaact cctgtttatg ttatgtgttt gttgtttttt
     4921 tatgctgcac gccgggcgcg ggaaggtcaa taaagatgca ggaatttcat attgcggtgg
     4981 ggtaaaagcc ggatgacatg gctcatccgg ctgagaaaag aattaaaatg cctgattaac
     5041 gcgcgccagg ccgtcgctga tagttgccgc ttcacccgta gcctccaggc agcaagccat
     5101 ctggattttc agcgattcgg gaatcggttc gctgccagca aggcaacgct caatccactg
     5161 cgccgtggtt tctgggtctt ttgcttgtgg cagtaactcg ctaccagcag tgtcctgttt
     5221 ttcatacagc acccgcatcc cttcacggtc aatgagattg atctgcgggc agcgctgcgg
     5281 attagcatac acttcgcctt cagtaccatg cattagtagc gcccgcccgc cgatatcgct
     5341 aaagaacttc gcgactcgcc cgatgtattc cggatgcgaa acgctggaaa gacgcagcgc
     5401 ctcaccttcg gcaaatggcg tcgccagttt cgccagggta tgtgcactgt tacgcacgcc
     5461 catccgccag cgcatcgcca gttgtttttc cagcggcggg caaaacgcgc cgacaggcat
     5521 aaacaccggt tgatgttcgt cgagcttcgc ctgcgcctgc ccgccgtgca acgttggtgt
     5581 aatacccatc agttcgaaaa tggtttctgt cagcacgcga gttggatctt cgctaacccc
     5641 gtgaaccacc acaggaaaac cgagtttgtg caggagaata gccaacaacg gcgtcaggtt
     5701 ggcctgttta cgcgcgccgt tgtaactggg gatgacaatc ggcatcggct tgcctgcagg
     5761 cggcgtcagc ttgatggtgt gattttgcat ggcttcgtaa aagccgagca tctctgcttc
     5821 cccttcccct ttgatacgta gcgcaatcaa tacgccgccc aactcaaggt cagggacttc
     5881 accattgagc atatgggcat acagaccgcg cgcagtatcc cggtctaaat cgcgcgcgtg
     5941 gtttttcccg cgcccgatct ctttaatgat tttgcgatag tccatttacg actccttacc
     6001 tgactcacat cattaacgcc gccgacggcg tggggatttc gttttttctt tctttttaac
     6061 tataccttca gggacttccg gttgctctat cggaaatacc ggtaatgcat ccagtagtcg
     6121 cttgccataa tttttggtca gcaagcgttt gtcgtagata accacttcgc cccagcaacc
     6181 gtggcttcga atcagtcgcc caacctgctg aatcaggtta aacgaggcgc tcggcaggct
     6241 ttgcacctca aacggatagc ggttgaggct tttcagccat tccccttcgg tgatcaccac
     6301 cgggctgtcg atgggcggaa aagcgatttt gtggatatgc acctggctga gcagatcacc
     6361 tttcaaatca agcccttcgg caaatgactg taagcccacc agcacactac gctcaccgtt
     6421 ggcaacgcgt ttgcggtgca gttcaactaa gcgataacgc ggctgatcgc cctgcaccag
     6481 cagcatcagg cgtaaatccg tcacatagtc gagaaagcgc tgcatcgccc gtccgctggc
     6541 aaacagtacc aacataccga gatgtttttt gctctccacc tgctcacgga aaaaggccgc
     6601 catttcggca atatgctgct cttcgttgtc gatggaaggc tcaaagcgca tccggggaat
     6661 aacaattttg ccctgttcgc agtggttaaa gggggaatcc agcgccacaa aacggtcgcc
     6721 cgctttctct ttcagcccgc tcatctcctg caaacgcgaa aaactgttca gcgaacgcaa
     6781 ggttgcggag gtgacaataa tgtgcggaat actgcgccac agcagccttt ccagctgatc
     6841 gctgacacgt attcccacgc agtgaaacca gagatgcagc tgcccttcgc gctcttcccg
     6901 cgtcgcccat ttggtcaccg gcgcaccgga agattgcgcc agagacgcca gccgccagag
     6961 tttgctttgc gcctcgaaca tccccaacgc gcggttcatc tgcaaaatca accgatgcag
     7021 acgcacaata tcgtggcggc cggttttctc acttaaatcg ttaagaaata actccgccag
     7081 accacgcagc atctcggtga gttttgccag ccgctggcag atctccagca cttcatcagg
     7141 cagttcgccc atcgcaaaac ggtgctctgc ctcctgcccg gcaggcatgt agagattgag
     7201 aatgttgttt aacgaggcga taagctcata caactcttca caatgcgcat tcaaacgttc
     7261 agggatcgcc agcggtggga tggtcttcgg gcgaaactgc tccatgcagg tagcgaccag
     7321 tttcgtgaac aagtccagct gtagtcgata ccagggcgcg gtgatttcag cgctcatctc
     7381 cagcgcatcc cgggccacat ctggcaggtg atgaccttcg tccagcacca gcagcagatt
     7441 tttcgggtca ggcagtacgg cttcgctttc catcgccgcc atcaccagcg catggtttgc
     7501 caccaccact tccgcttcct gaatctcccg acgagcaaca aaaaacgggc attcacgata
     7561 gtagtaacag ttacggttaa ggcagctggc tttgtcggta ctcaaacgac gccagagatc
     7621 atcatctata gcgatatccg tatgatcgcg cagaccatcc catttataag tgtcgagatc
     7681 gcccttcagc ttcgcacaac gtttttgctc ttcctgatta tttggcgtca gttcgtcgtc
     7741 aagaaacgcc agcagatcct gttgcgtggg ttcagtgctg gcgagcgccg tcagattacg
     7801 cggacaaacg tagcgcccac gcccgaaagc ggcagtgaat ttaagatcgg gaatgatctt
     7861 tttcagcagc ggtaaatctt tgctgtaaat ctgatcctgc aacgccacgt tggcggtact
     7921 caccaccagc gttttttgct cttcgcgggc aatggcgatg ccggggatca aataggagag
     7981 cgttttccca acgccggtgg gggcttcaat cgccagatgc cgcccttctt ctccggccag
     8041 cgttttggcg acgtccgcaa tcatctgccg ctgcggcgca cggggaataa agtcggggat
     8101 ctgttcctga agcgccttat accaggcggc aatttgcgct ttaagcgcgg cggttaatgc
     8161 catgagaaaa cctgaaatac tgtataaaca gccaatattg tggcattttt cggttttcag
     8221 gggtaatggt ttttctgttt ctggaaagcg gctcgcaata accaccaaca taatgcggtt
     8281 taatgcccgt tcgagcacaa aggatccaac acgtcatgca agacaccatc ataaatttct
     8341 acagcaccag cgatgactac ggtgattttt ctaactttgc cgcctggccc atcaaggttg
     8401 acgggaaaac ctggcccacc tccgaacact attttcaggc acaaaaattc cttgatgaaa
     8461 aataccgcga agagatccgt cgggtttctt cccccatggt cgccgctcgc atggggcgta
     8521 atcgttccaa gccacttcgt aaaaactggg agtcggttaa agaacaggtg atgcgtaaag
     8581 cacttcgtgc taaattcgaa cagcatgcag aactgcgtgc gctcttgctg gcaaccgcgc
     8641 ccgcaaaact ggttgagcat acggaaaacg atgcttactg gggagacggt ggtaatggta
     8701 agggcaagaa tcgactgggc taccttttaa tggagttgcg cgaacaattg gctatagaga
     8761 agtaacgtct cggtggctta tgggcggtaa aacagataaa ttttttcccg tgttctcaaa
     8821 ttgctgacaa gtgcttgttg tgaaatgccg gatgcggcgt aaacgcctta tccggcctac
     8881 aaaagcatgc aaattcaata tattgcagag ttaacgtagg cctgataagc gtagcgcatc
     8941 aggcagtttt gcgtttgttc atcagcctga tgccgggcat agcccggcat aaaagattac
     9001 tgcgcagcgg caggtttacg cggacggcgg cgacgttgtt gttcgcctgc cggtttggca
     9061 tcgccgaggc ggcgagacgg tttttctgca ggtttcgcgc ttgcagattt tgcgccacct
     9121 tccccacggc gtggttgctg ttgaccgcga ccaccacctt gcccacgacc gccgccgcca
     9181 cgttgctggc gaccgttctg gatcggttcg gctttgattg acgggtccgg ctcatagccc
     9241 ggaatcgcaa tgcgcgggat ctcttttttc agcagttttt cgatatcacg cagcagtttg
     9301 tgttcatcaa cacacaccag cgacaacgct tcaccggtag cagccgcacg accggtacgc
     9361 ccgatacggt ggacataatc ttccggtacg tttggcagtt catagttgac gacgtgcggc
     9421 aactcttcga tatccagacc gcgcgcagcg atatcggtcg ccaccagtac gcgaatgtca
     9481 ctcgatttaa aatcagccag cgcacgggta cgcgcacctt gcgatttatt cccgtggatc
     9541 gccgcactac ggatgccatc tttattgagc tgctcagcca gatggttagc gccgtgtttg
     9601 gtacgggtaa acaccagcac ctgctgccag ttccctttac caatcatgtg cgacagcaat
     9661 tcgcgtttgc gtttcttatc gacaaagtga acgtgctggg tcacctgatc agacgcggta
     9721 ttgcggcgtg ccacttcgat ttccagcggg ttgtgcaaca gtttttccgc cagggcttta
     9781 atatcgtcag agaaggtcgc agagaacagc aggttctggc gtttcgctgg cagttttgtt
     9841 aacacgcgac ggatatcgtg gataaagccc atgtcgagca tgcggtcagc ttcatcgagg
     9901 acgaggattt caacctgatc cagcttcact gcattttgat gttccaggtc cagcaaacgt
     9961 cccggtgttg ccaccagaac atcaacgccg ccgcgcagct tcatcatttg cgggttaatg
    10021 ctgacaccgc caaacaccac cagcgaacga atgttcaaat atttgctgta atcacggacg
    10081 ttttcgccaa tctgcgcagc cagttcacgg gtcggggtaa gaatgagcgc acgtaccgga
    10141 cgacgccctt tggcgtgcgg ctggcgagtg atcaggtgtt gcaacagcgg cagcgtaaag
    10201 cccgctgttt taccggtacc ggtctgggcg ctagccatca ggtcgcggcc ttccagcacc
    10261 gcagggatcg cctgctgctg aataggggtg ggttcacggt aaccctgctc ggcaacggcg
    10321 cgcaggatat cagggcttaa acccaaagaa tcgaaagaca taactactcc gaaccgcccc
    10381 gaccgttacc ggtgtagttt tcagggagat actgaaaagg aaaatgacaa aaaccacaat
    10441 gtcatgaggg agcggagtgt agcagttttt gtgacgcagc gcataaatta tccctgtgac
    10501 tggcgttgct aaaattctta gccatactgg acaactcccc tatggagtca taatcttaat
    10561 caatcatttg attaagaatg gactgtgcga tgaataatcc tgccatgaca atcaagggtg
    10621 aacaggcgaa aaaacagctg attgctgccg cactggcgca gtttggtgaa tatggaatga
    10681 acaccaccac tcgcgagata gccgcccagg ccgggcagaa tatcgctgcc atcacctact
    10741 acttcggttc gaaagaagat ttgtacctcg cctgcgccca gtggattgcc gattttattg
    10801 gcgagcagtt ccgtccgcat gccgaggaag ccgaacgctt gttcgcgcaa ccacagcctg
    10861 atcgggctgc catccgtgaa ctgatccttc gcgcctgcag gaacatgatt aagctgctca
    10921 cccaggatga caccgtcaac ctcagcaagt ttatctcccg tgagcagctc tctcccacgg
    10981 cagcctacca cctggtgcac gaacaggtga ttagtccgct acacagccac ctgacacgcc
    11041 tgattgccgc ctggaccggc tgcgacgcca atgacacccg catgatcctt catacccatg
    11101 cgttgattgg tgagattctg gcgttccgtc ttggcaaaga aacaattctg ttacgtaccg
    11161 gctggaccgc gttcgatgaa gaaaagaccg aactgatcaa ccagacggtg acctgtcata
    11221 tcgacctgat tttgcaagga ttatcgcaaa ggagtttgta gtgatgaaaa aacctgtcgt
    11281 gatcggactg gcggtagtgg tacttgccgc cgtggttgcc ggaggctact ggtggtatca
    11341 aagccgccag gataacggcc tgaagctgta tggcaacgtg gatattcgta cggtgaatct
    11401 tagtttccgt gttggggggc gcgttgaatc gctggcggtg gacgaaggtg atgcgatcaa
    11461 agcgggccag gtgctgggcg aactggatca caagccgtat gagattgccc tgatgcaggt
    11521 gaaagcgggc gtctcggtgg cacaggcgca gtatgacctg atgcttgccg ggtatcgcga
    11581 tgaagaaatc gctcaggccg ccgcagcggt gaaacaggcg caagccgcct atgactatgc
    11641 gcagaacttc tataaccgcc agcaaggact gtggaaaagc cgcaccattt cggcaaatga
    11701 cctggaaaat gcccgctcct cgcgcgacca ggcacaggca acgctgaaat cagcacagga
    11761 taaattgcgt cagtaccgtt ccggtaaccg tgaacaggac atcgctcagg cgaaagccag
    11821 cctcgaacag gcgcaggcgc aactggcgca ggcggagctg aatttacagg actcaacgtt
    11881 gatagcaccg tctgatggca cactgttaac gcgcgcggtg gagccaggca cggttctcaa
    11941 tgaaggtggc acggtgttta ccgtttcact aacgcgtccg gtgtgggtgc gcgcttatgt
    12001 tgatgaacgg aatcttgacc aggcccagcc ggggcgcaaa gtgctgcttt ataccgatgg
    12061 tcgcccggac aagccgtatc acgggcagat tggtttcgtt tcgccgactg ctgaatttac
    12121 cccgaaaacc gtcgaaacac cggatctgcg taccgacctc gtctatcgcc tgcgtattgt
    12181 ggtgaccgac gccgatgatg cgttacgcca gggaatgccg gtgacggtgc aattcggtaa
    12241 cgaggcagga catgaatgat gccgttatca cgctgaacgg cctggaaaaa cgctttccgg
    12301 gcatgggcaa gcccgccgtc gcgccgctcg attgtaccat tcacgccggt tatgtgacgg
    12361 ggctggtggg gccggatggt gcaggtaaaa ccacgctaat gcggatgctg gcgggattac
    12421 tgaaacccga cagcggcaat gccacgatga ttggctttga tccaatcaaa aacgacggcg
    12481 cgctgcacgc cgtgctcggc tatatgccgc agaaatttgg tctgtatgaa gatctcacgg
    12541 tgatggagaa cctcaatctg tacgcggatt tgcgcagcgt caccggcgag gcacgtaagc
    12601 aaacttttgc tcgcctgctg gagtttacgt ctcttgggcc gtttaccgga cgcctggcgg
    12661 gcaagctctc cggtgggatg aaacaaaaac tcggtctggc ctgtaccctg gtgggcgaac
    12721 cgaaagtgtt gctgctcgat gaacccggcg tcggcgttga ccctatctca cggcgcgaac
    12781 tgtggcagat ggtgcatgag ctggcaagcg aagggatgtt aatcctctgg agtacctcgt
    12841 atctcgacga agccgagcag tgccgtgacg tgttactgat gaatgaaggc gagttgctgt
    12901 atcaggggga accaaaagcc ctgacacaaa ccatggccgg acgcagcttt ctgatgacca
    12961 gtccacacga gggcaaccgc aaactgttgc aacgcgcctt gaaactgcca caggtcagcg
    13021 acggcatgat tcaggggaaa tcggtacgtc tgatcctcaa aaaagaggcc acaccagacg
    13081 atattcgcca tgccgacggg atgccggaaa tcaacatcaa cgaaactacg ccgcgttttg
    13141 aagatgcatt tattgatttg ctgggtggtg ccggaacctc agaatcgccg ctgggtgcaa
    13201 tattgcatac ggtagaaggc acacccggcg agacggtgat cgaagcgaaa gaactgacca
    13261 agaaatttgg tgattttgcc gccaccgatc acgtcaactt tgccgttaaa cgtggggaga
    13321 tttttggttt gctggggcca aacggcgcgg gtaaatcgac cacctttaag atgatgtgcg
    13381 gtttgctggt gccgacttcc ggccaggcgc tggtgctggg gatggatctg aaagagagtt
    13441 ccggtaaagc gcgccagcat ctcggctata tggcgcaaaa attttcgctc tacggcaacc
    13501 tgacggtcga acagaattta cgctttttct ctggtgtgta tggcttacgc ggtcgggcgc
    13561 agaacgaaaa aatctcccgt atgagcgagg catttggcct gaaaagtatc gcctcccacg
    13621 cgaccgatga actgccatta ggttttaaac agcggctggc gctggcctgt tcgctgatgc
    13681 atgaaccgga cattctgttt ctcgacgaac caacgtccgg cgttgatccc ctcacccgcc
    13741 gcgaattttg gctgcatatc aacagcatgg tagagaaagg cgtcacggtg atggtcacca
    13801 ctcactttat ggatgaagca gaatattgcg accgcatcgg cctggtgtac cgtgggaaat
    13861 taattgccag cggcacgccg gatgatttga aagcgcagtc ggccaacgac gagcaacccg
    13921 atcccaccat ggagcaagcc tttattcagt tgatccacga ctgggataag gagcatagca
    13981 atgagtaacc cgatcctgtc ctggcgtcgc gtacgggcgc tgtgcgttaa agagacgcgg
    14041 cagatcgttc gcgatccgag tagctggctg attgcggtag tgatcccgct gctactgctg
    14101 tttatttttg gttacggcat taacctcgac tccagcaagc tgcgggtcgg gattttactg
    14161 gaacagcgta gcgaagcggc gctggatttc acccacacca tgaccggttc gccctacatc
    14221 gacgccacca tcagcgataa ccgtcaggaa ctgatcgcca aaatgcaggc ggggaaaatt
    14281 cgcggtctgg tggttattcc ggtggatttt gcggaacaga tggagcgcgc caacgccacc
    14341 gcaccgattc aggtgatcac cgacggcagt gagccgaata ccgctaactt tgtacagggg
    14401 tatgtcgaag ggatctggca gatctggcaa atgcagcgag cggaggacaa cgggcagact
    14461 tttgaaccgc ttattgatgt acaaacccgc tactggttta acccggcggc gattagccag
    14521 cacttcatta tccccggtgc ggtgaccatt atcatgacgg tcatcggcgc gattctcacc
    14581 tcgctggtgg tggcgcgaga atgggaacgc ggcaccatgg aggctctgct ctctacggag
    14641 attacccgca cggaactgct gctgtgtaag ctgatccctt attactttct cgggatgctg
    14701 gcgatgttgc tgtgtatgct ggtgtcagtg tttattctcg gcgtgccgta tcgcgggtcg
    14761 ctgctgattc tgttttttat ctccagcctg tttttactca gtaccctagg gatggggctg
    14821 ctgatttcca cgattacccg caaccagttc aatgccgctc aggtcgccct gaacgccgct
    14881 tttctgccgt cgattatgct ttccggcttt atttttcaga tcgacagtat gcccgcggtg
    14941 atccgcgcgg tgacgtacat tattcccgct cgttatttcg tcagcaccct gcaaagcctg
    15001 ttcctcgccg ggaatattcc agtggtgctg gtggtaaacg tgctgttttt gatcgcttcg
    15061 gcggtgatgt ttatcggcct gacgtggctg aaaaccaaac gtcggctgga ttagggagaa
    15121 gagcatgttt catcgcttat ggacgttaat ccgcaaagag ttgcagtcgc tgctgcgcga
    15181 accgcaaacc cgcgcgattc tgattttacc tgtgcttatt caggtgattc tgttcccgtt
    15241 cgccgccacg ctggaagtga ctaacgccac catcgccatc tacgatgaag ataacggcga
    15301 gcattcggtg gagctgaccc aacgttttac ccgcgccagc gcctttactc atgtgctgct
    15361 gctgaaaagc ccacaggaga tccgcccaac catcgacaca caaaaggcgt tactcctggt
    15421 gcgtttcccg gctgacttct cgcgcaaact ggataccttc cagaccgcgc cgttgcagtt
    15481 gatcctcgac gggcgtaact ccaacagtgc gcaaattgcc accaactacc tgcaacagat
    15541 cgtcaaaaat tatcagcagg aactgctgga aggaaaaccg aaacctaaca acagcgagct
    15601 ggtggtacgc aactggtata acccgaatct cgactacaaa tggtttgtgg tgccgtcact
    15661 gatcgccatg atcaccacta tcggcgtaat gatcgtcact tcactttccg tcgcccgcga
    15721 acgtgaacaa ggtacgctcg atcagctact ggtttcgccg ctcaccacct ggcagatctt
    15781 catcggcaaa gccgtaccgg cgttaattgt cgccaccttc caggccacca ttgtgctggc
    15841 gattggtatc tgggcgtatc aaatcccctt cgccggatcg ctggcgctgt tctactttac
    15901 gatggtgatt tacggtttat cgctggtggg attcggtctg ttgatttcat cactctgctc
    15961 gacacaacag caggcgttta tcggcgtgtt tgtctttatg atgcccgcca ttctcctttc
    16021 cggttacgtt tcgccggtgg aaaacatgcc agtatggctg caaaacctga cgtggattaa
    16081 ccctattcgc cactttacgg acattaccaa gcagatttat ttgaaggatg cgagtctgga
    16141 tattgtgtgg aatagtttgt ggccgctact ggtgataacg gccacgacag ggtcagcggc
    16201 gtacgcgatg tttagacgta aggtgatgta acttcttatc tttcgccagc aaagacacta
    16261 ccgccgggcc ggcaaggatt gccagccctg caattgccag caaaaagttg ttttccagta
    16321 cgcgggacaa cagccacagc acaatcagcg ctgcgccaaa ataccaggtc gtggtcagct
    16381 cttccaacac gtctgccacc tcacgccagc gttgctcggg gtggcgctga aacaccaaca
    16441 tcatcactac cgtgacacaa tacagtgcgc ataatcccac gccgacgtga accagagtct
    16501 tactcatcca gcccaccacc agcagcgcca ctaccagtaa atgcaacata atctgccagc
    16561 aacttagacc cgttgcgaca cgaacacgtt gttgccactt catggcttct ctcctgaaga
    16621 atttatctct acttactcag agtaccgaac tttacggaaa attccgtaag accgcacctt
    16681 tttgtgacgc cgactacact atttcaatga gataagaggc aaagacagga aaattatgcc
    16741 cgatcaaaca caacaatttt cgttcaaggt gctcaccatc aacattcaaa aaggttttac
    16801 cgcgtttaac cgacgcttca ttttgccgga acttcgcgac gcggtgcgta ccgtcagcgc
    16861 cgatattgtt tgcctgcagg aagtgatggg cgcgcacgaa gttcatccgc tgcatgtgga
    16921 aaactggccc gatacctcgc actacgagtt tctcgccgac actatgtgga gcgattttgc
    16981 ctacggtcgc aatgccgtat acccggaagg gcatcacggc aacgccgtac tgtcgcgtta
    17041 tcccattgaa cattatgaga atcgcgatgt ttcggtcgat ggtgcggaaa agcgcggcgt
    17101 gctctactgc cgcattgtgc cgccgatgac cggaaaagcg attcatgtga tgtgcgtaca
    17161 tctgggcctg cgtgaggcgc accgtcaggc gcagcttgcg atggggtgaa tgagctaccg
    17221 gacggcgaac cggtattggt ggcgggtgat ttcaatgact ggcggcaaaa agctaatcat
    17281 ccgttaaaag tgcaggccgg actggatgag atttttaccc gcgcccacgg acgcccggcg
    17341 cgcacgtttc cggtgcaatt tcctctacta cgactggaca ggatctacgt caaaaatgcc
    17401 agcgccagcg cgccaaccgc gttgccgctg cggacatggc gacacctttc tgatcatgcc
    17461 cctttaagtg cggagattca tttatgaaat gtagctggcg cgaaggcaat aagatccagt
    17521 tgctggaaaa cggcgagcaa tattatcccg cggtgtttaa ggcgattggc gaggcacaag
    17581 aacgcatcat tcttgaaacg tttatctggt ttgaggataa cgtcggcaaa cagctgcatg
    17641 cggcactact ggcagcagcg caacgcgggg ttaaagcgga agtcttgctg gatggctacg
    17701 gttcgccgga tctcagcgat gagtttgtca ataaactgac ggcagctggc gtagtgttcc
    17761 gctactacga tccccgccct cgcctttttg gtatgcgcac caatgtgttt cgccggatgc
    17821 atcgcaaaat tgtggtgatc gacgcgcgta tcgcctttat tggcgggctg aattactccg
    17881 ccgagcatat gtccagctac ggtccagagg ctaaacagga ttacgcggta cgccttgaag
    17941 ggccgattgt cgaagatatt ctccagtttg agctggaaaa cctgcctgga cagagcgcgg
    18001 cacggcgctg gtggcgacgt catcacaaag cggaagagaa ccgccagccg ggagaagcgc
    18061 aggtattgct ggtctggcgc gataacgaag aacatcgcga tgatattgaa cgccattatc
    18121 tgaaaatgct cactcaggcg cggcgggaag tgattatcgc caacgcctac ttcttccccg
    18181 gctatcgatt tttacacgcc ttgcgtaaag cggcacggcg cggggtgcgg atcaaactga
    18241 tcattcaggg cgaaccggat atgccgattg tcagagtcgg tgcgcgcttg ctgtataact
    18301 atctggttaa aggcggcgtt caggtttttg agtaccgccg ccgcccgctc cacggcaaag
    18361 tggcattgat ggacgatcac tgggcgacag tagggtccag taatctcgat ccgctcagtt
    18421 tgtcactgaa tctcgaagca aatgtcatca tccacgatcg tcattttaac cagacgctgc
    18481 gcgataatct gaacggcatt attgccgcag attgtcagca ggtggatgaa accatgctgc
    18541 ccaaacgcac ctggtggaac ctgaccaaaa gcgtgctggc gttccacttt ttacgccact
    18601 tcccggcgct ggttggctgg catccggcac acacaccacg tctgacgcag gttgatcccc
    18661 ccgcacaacc gacaatggaa acgcaggatc gggtagaaac tgaaaacacg ggggtaaaac
    18721 cctgatgagt aaatcacacc cgcgctagcg cttagcaaag aagatcctca cctggctgtt
    18781 ttttatcgcg gtgattgtgt tgctggtggt ctacgccaaa aaagtggact gggaagaggt
    18841 ctggaaggtc atccgcgact acaatcgcgt tgcgctgctt agtgcggtcg ggctggtggt
    18901 cgtcagctat ctgatttacg gctgctatga cctgctcgcc cgcttttact gcggtcacaa
    18961 actggcgaag cgccaggtga tgctggtgtc gtttatctgc tacgccttca acctgacgct
    19021 cagtacctgg gtcggcggca ttggtatgcg ctatcgtttg tactcccgac tagggttacc
    19081 gggcagcact attacgcgga ttttctcgct cagtattacc accaactggc tgggctatat
    19141 tttactggca gggattatct ttaccgcagg cgtggtggag ttgccggatc actggtatgt
    19201 cgctcaaact acgctgcgta ttctcggcat tggcttactg atgattatcg cggtttattt
    19261 gtggttttgc gctttcgcga agcaccgcca tatgaccatc aaagggcaaa agctggtgct
    19321 gccttcatgg aaattcgccc tcactcagat gctgatttcc agcgttaact ggatggtaat
    19381 gggggcgatt atctggctgt tacttggtca aagcgtgaac tatttctttg tactgggcgt
    19441 gttactggtt agtagtattg ctggcgtcat cgtgcatatt ccggcgggga tcggtgtgct
    19501 ggaagcggtg tttatcgcac tactggctgg ggagcatacc tcgaaaggga gcattatcgc
    19561 cgcgctgctc gcttatcgcg tcctgtatta ctttatcccg ctgctgctgg cgctgatctg
    19621 ttacctgcta ctggaaagcc aggcgaagaa gctacgggcg aaaaatgaag cggcgatgtg
    19681 aaatttagtt aagttcttta gtatctgcat tcacgactaa tgaaaaaaac gcgtatgcct
    19741 ttgccagaca agcgttatag ctatcgcggc aagtatccca aagaagccga taaaatagta
    19801 atagagtgtt aatgcacctg taaaagcgca acgaaccgga ctccacaact cttcactatg
    19861 gcattttcct gccagttcaa ggagcttatt tcgtccatgc gagataatgc ctggccataa
    19921 cacaatcact aagcaactta ttacccaaat gggccgttca ctcattaatg ccgcattcac
    19981 gatgattacc agggccaacc ctgtcaacgt catcataatg ataaaacggt gagaaccagg
    20041 atcaacatta aataataaac cggccagcat actgattaaa aacatcgcgc ccgcagtgct
    20101 gaacacaatg ata
//
LOCUS       DAZPEB010000057        20071 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZPEB010000057 DAZPEB010000000
VERSION     DAZPEB010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20071
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.058"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(301..1113)
                     /locus_tag="ACHURJ_001824"
     CDS             complement(301..1113)
                     /locus_tag="ACHURJ_001824"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFR1280796.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGMHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKRS"
     gene            1375..2136
                     /gene="udp"
                     /locus_tag="ACHURJ_001825"
     CDS             1375..2136
                     /gene="udp"
                     /locus_tag="ACHURJ_001825"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFR1280797.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            2277..3704
                     /gene="rmuC"
                     /locus_tag="ACHURJ_001826"
     CDS             2277..3704
                     /gene="rmuC"
                     /locus_tag="ACHURJ_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFR1280798.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDAHSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            3799..4554
                     /gene="ubiE"
                     /locus_tag="ACHURJ_001827"
     CDS             3799..4554
                     /gene="ubiE"
                     /locus_tag="ACHURJ_001827"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFR1280799.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            4568..5173
                     /gene="ubiJ"
                     /locus_tag="ACHURJ_001828"
     CDS             4568..5173
                     /gene="ubiJ"
                     /locus_tag="ACHURJ_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFR1280800.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            5170..6810
                     /gene="ubiB"
                     /locus_tag="ACHURJ_001829"
     CDS             5170..6810
                     /gene="ubiB"
                     /locus_tag="ACHURJ_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFR1280801.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            6889..7158
                     /gene="tatA"
                     /locus_tag="ACHURJ_001830"
     CDS             6889..7158
                     /gene="tatA"
                     /locus_tag="ACHURJ_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFR1280802.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            7162..7677
                     /gene="tatB"
                     /locus_tag="ACHURJ_001831"
     CDS             7162..7677
                     /gene="tatB"
                     /locus_tag="ACHURJ_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFR1280803.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELIQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            7680..8456
                     /gene="tatC"
                     /locus_tag="ACHURJ_001832"
     CDS             7680..8456
                     /gene="tatC"
                     /locus_tag="ACHURJ_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFR1280804.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            8498..9280
                     /gene="tatD"
                     /locus_tag="ACHURJ_001833"
     CDS             8498..9280
                     /gene="tatD"
                     /locus_tag="ACHURJ_001833"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFR1280805.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAVQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDFTPKPSS
                     RRNEPAHLHHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(9277..9765)
                     /gene="rfaH"
                     /locus_tag="ACHURJ_001834"
     CDS             complement(9277..9765)
                     /gene="rfaH"
                     /locus_tag="ACHURJ_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFR1280806.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLALMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            9932..11425
                     /gene="ubiD"
                     /locus_tag="ACHURJ_001835"
     CDS             9932..11425
                     /gene="ubiD"
                     /locus_tag="ACHURJ_001835"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFR1280807.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGVPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            11471..12172
                     /gene="fre"
                     /locus_tag="ACHURJ_001836"
     CDS             11471..12172
                     /gene="fre"
                     /locus_tag="ACHURJ_001836"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFR1280808.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDYQIVVDIPYGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLSELEALSLKHP
                     GLRVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(12364..13527)
                     /gene="fadA"
                     /locus_tag="ACHURJ_001837"
     CDS             complement(12364..13527)
                     /gene="fadA"
                     /locus_tag="ACHURJ_001837"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFR1280809.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCN
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(13537..15740)
                     /gene="fadB"
                     /locus_tag="ACHURJ_001838"
                     /pseudo
     CDS             complement(13537..15740)
                     /gene="fadB"
                     /locus_tag="ACHURJ_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
     gene            15930..17261
                     /gene="pepQ"
                     /locus_tag="ACHURJ_001839"
     CDS             15930..17261
                     /gene="pepQ"
                     /locus_tag="ACHURJ_001839"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFR1280810.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHASVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHLQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFSGIRIEDNVVIHENNVENMTRDLKLA"
     gene            17261..17875
                     /locus_tag="ACHURJ_001840"
     CDS             17261..17875
                     /locus_tag="ACHURJ_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFR1280811.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGIGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            17914..19365
                     /gene="trkH"
                     /locus_tag="ACHURJ_001841"
     CDS             17914..19365
                     /gene="trkH"
                     /locus_tag="ACHURJ_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFR1280812.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            19377..19922
                     /gene="hemG"
                     /locus_tag="ACHURJ_001842"
     CDS             19377..19922
                     /gene="hemG"
                     /locus_tag="ACHURJ_001842"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFR1280813.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMSSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAQLTNKPTLK"
BASE COUNT         4672 a         5068 c         5480 g         4851 t
ORIGIN      
        1 tgtgaaaaac aaaatgtttc actttaatat ctttttatca ggaaggtctg agatgattta
       61 taattaatgt gaatgtgttg ataataatta atgtttgttt gtgaatggga gtagatggta
      121 aatctctaag gtgtcttcca ttgaagagac tcatttatga ctatgacttg ccgaccttct
      181 taaggtcaaa tagcggagtc gtaatctcca atcaattcat actgattatt ttttggggga
      241 cagagatggc agcaaaacgc actcgttcac tcaccatctc aaatatagac taagaggaaa
      301 ctaactccgc ttcccaccat actgcttaaa ccattccagc atacgctgcc agccatcttt
      361 cgcagattcg gcatgatagc tcgggcgata atcagcgttg aatgcatgcc cggcgtccgg
      421 gtacacgata atctctgctt tcgcattagc agcccgcagc gcctggcgca tggtttcaac
      481 gctctcctgc ggaatgctgt tatcctgacc accatataag ccgagaaccg gcgcgttaag
      541 atcggttgcg atatcaacag gttgtttcgg tgaatttagc gacttgtcgc ccgtcagttt
      601 gccgtaccac gccactgcag cttttagctg tggattatgc gcggcataca gccaggtgat
      661 acgtccaccc cagcagaatc cggtgatcat taaacgatga acatcgccgc cgttgcggga
      721 agcccaactg gcgacatgat cgagatcggc cagcacctgc gagtcaggca cttttgctac
      781 cagaccgcta agcaacgtgg ggatatcggc aaaatcattc ggatcgcctt cgcggaagta
      841 aagttcaggt gcgatagcca gatacccctc cagcgccaga cggcgacaaa tgtcgcggat
      901 atgttcatgc atgccaaaaa tttcctgcac tacaatgacc actggcagtg ggccatcgct
      961 ttgctttggt ctggcatggt aagcaggcat gttatcccct tgtgaaggga tagaggtgaa
     1021 gcccgccaca atcgcgtcgt ccggggtctg aacgatggtc gaagcgagag gcgatgcagc
     1081 aggtgcaaat ccagattgtt gtgttgttgc catggtattc tccgtaccct tataaaaatg
     1141 ttgcgcaatg ttaactatag tcagcatgca acaaatcaca ttgcctgaat cggctcatct
     1201 tttatgcagt cctgcagaat gaagagtgat ttatgtgatt tgcatcactt ttggtgggta
     1261 aatttatgca acgcatttgc gtcatggtga tgagtatcac gaaaaaatgt taaacccttc
     1321 ggtaaagtgt ctttttgctt cttctgacta aaccgattca cagaggagtt gtatatgtcc
     1381 aagtctgatg tttttcatct cggcctcact aaaaacgatt tacaaggggc tacgcttgcc
     1441 atcgtccctg gcgacccgga tcgtgtggaa aagatcgccg cgctgatgga taagccggtt
     1501 aagctggcat ctcaccgcga attcaccacc tggcgtgcag agctggatgg taaacctgtt
     1561 atcgtctgct ctaccggtat cggcggcccg tctacctcta ttgctgttga agagctggca
     1621 cagctgggca ttcgcacctt cctgcgtatc ggtacaacgg gcgctattca gccgcatatt
     1681 aatgtgggtg atgtcctggt taccacggcg tctgtccgtc tggatggcgc gagcctgcac
     1741 ttcgcaccgc tggaattccc ggctgtcgct gatttcgaat gtacgactgc gctggttgaa
     1801 gctgcgaaat ccattggcgc gacaactcac gttggcgtga cagcttcttc tgataccttc
     1861 tacccaggcc aggaacgtta cgatacttac tctggtcgcg tagttcgtca ctttaaaggt
     1921 tctatggaag agtggcaggc gatgggcgta atgaactatg aaatggaatc tgcaaccctg
     1981 ctgaccatgt gtgcaagtca gggcctgcgt gccggtatgg tagcgggtgt tatcgttaac
     2041 cgcacccagc aagagatccc gaatgctgag acgatgaaac aaactgaaag ccaagcggtg
     2101 aaaatcgtgg tggaagcggc gcgtcgtctg ctgtaattct cttctcctgt ctgaaggccg
     2161 acgcgttcgg ccttttgtat ttttgcgtag cgcctcgcag gaaatgcctt tccaactgga
     2221 cgtttgtaca gcacaattct attttgtgcg ggtaagttgt tgcgtcagga ggcgttgtgg
     2281 atttctcaat catggtttac gcagttattg cgttggtggg tgtggcaatt ggctggctgt
     2341 ttgccagtta tcaacatgcg cagcaaaaag ccgagcaatt agctgaacgt gaagagatgg
     2401 tcgcggagtt aagcgcggca aaacaacaaa ttacccaaag cgagcactgg cgtgcagagt
     2461 gcgagttact caataacgaa gtgcgcagcc tgcaaagtat taacacctct ctggaggccg
     2521 atctgcgtga agtaaccacg cggatggaag ccgcacagca acatgctgac gataaaattc
     2581 gccagatgat taacagcgag cagcgcctca gtgagcagtt tgaaaacctc gccaaccgta
     2641 tttttgagca cagcaatcgc cgggttgatg agcaaaaccg tcagagtctg aacagcctgt
     2701 tgtcgccgct acgtgaacaa ctggacggtt tccgccgtca ggttcaggac agcttcggta
     2761 aagaagcaca agaacgccat accctgaccc acgaaattcg caatctccag caactcaacg
     2821 cgcaaatggc ccaggaagcg atcaacctga cgcgcgcgct gaaaggcgac aataaaaccc
     2881 agggcaactg gggcgaggta gtattgacgc gggtgctgga ggcttccggt ctgcgtgaag
     2941 ggtatgaata tgaaacccag gtcagcatcg aaaatgacgc ccactcgcgg atgcagccgg
     3001 atgtcatcgt gcggctgccg cagggaaaag atgtggtgat cgacgccaaa atgacgctgg
     3061 tcgcctatga acgctatttt aacgccgaag acgactacac ccgcgaaagc gcgctacagg
     3121 aacatatcgc gtcggtgcgt aaccatatcc gtttgctggg acgcaaagat tatcaacagc
     3181 tgccggggct gcgaactctg gattacgtgc tgatgtttat tcccgttgaa cccgcttttt
     3241 tactggcgct tgaccgccag ccggagctga tcaccgaagc gttgaaaaac aatatcatgc
     3301 tggttagccc gactacgctg ctggtggcgt tgcgtactat tgccaacctg tggcgttatg
     3361 agcatcaaag ccgcaacgcc cagcaaatcg ccgatcgtgc cagcaagctg tacgacaaga
     3421 tgcgtttgtt catcgatgac atgtccgcga ttggtcaaag tctcgacaaa gcgcaggata
     3481 actaccgcca ggcaatgaaa aaactctctt caggtcgcgg aaatgtgctg gcgcaggcag
     3541 aagcgtttcg cggtttagga gtagaaatta aacgcgagat taatccggat ttggctgaac
     3601 aggcggtgag ccaggatgaa gagtatcgac ttcggtcggt tccggagcag ccgaatgatg
     3661 aagcttatca acgcgatgat gaatataatc agcagtcgcg ctagcccatt aggggtagtt
     3721 aagccgggta gaaatctagg gcatcgacgc ccaatctgtt acacttctgg aacaattttt
     3781 tgatgagcag gcattgagat ggtggataag tcacaagaaa cgacgcactt tggttttcag
     3841 accgtcgcga aggaacaaaa agcggatatg gtcgcccacg ttttccattc cgtggcatca
     3901 aaatacgatg tcatgaatga tttgatgtca tttggtattc atcgtttgtg gaagcgattc
     3961 acgattgatt gcagcggcgt acgccgtggg cagaccgtgc tggatctggc tggtggcacc
     4021 ggcgacctga cagcgaaatt ctcccgcctg gtcggagaaa ctggcaaagt ggtccttgct
     4081 gatatcaatg aatccatgct caaaatggga cgcgagaagc tgcgtaatat cggtgtgatt
     4141 ggcaacgttg agtatgttca ggcgaacgct gaggcgctgc cgttcccgga taacaccttt
     4201 gattgcatca ccatttcgtt tggtctgcgt aacgtcaccg acaaagataa agcactgcgt
     4261 tcaatgtatc gcgtgctgaa acccggcggc cgcctgctgg tgcttgagtt ctcgaagcca
     4321 attatcgagc cgctgagcaa agcctatgat gcatactcct tccatgtgct gccgcgtatt
     4381 ggctcactgg tcgcgaacga cgccgacagc taccgttatc tggcagaatc catccgtatg
     4441 catcccgatc aggataccct gaaagccatg atgcaggatg ccggattcga aagtgtcgac
     4501 tactacaatc tgacggcagg ggttgtggcg ctgcatcgtg gttataagtt ctgacaggag
     4561 accggaaatg ccttttaaac ctttagtgac ggcaggaatt gaaagtctgc tcaacacctt
     4621 cctgtatcgc tcacccgcgc tgaaaacagc ccgctcgcgt ctgctgggta aagtattgcg
     4681 cgtggaggta aaaggctttt cgacgtcatt gattctggtg ttcagcgaac gccaggttga
     4741 tgtactgggc gaatgggcag gcgatgctga ctgcaccgtt atcgcctacg ccagtgtgtt
     4801 gccgaaactt cgcgatcgcc agcagcttac cgcactgatt cgcagtggtg agctggaagt
     4861 gcagggcgat attcaggtgg tgcaaaactt cgttgcgctg gcagatctgg cagagttcga
     4921 ccctgcggaa ctgctggccc cttataccgg tgatatcgcc gctgaaggaa tcagcaaagc
     4981 catgcgcgga ggcgcaaagt tcctgcatca cggcattaag cgccagcaac gttatgtggc
     5041 ggaagccatt actgaagagt ggcgtatggc acccggtccg cttgaagtgg cctggtttgc
     5101 ggaagagacg gctgccgtcg agcgtgctgt tgatgccctg accaaacggc tggaaaaact
     5161 ggaggctaaa tgacgccagg tgaagtacgg cgcctatatt tcatcattcg cactttttta
     5221 agctacggac ttgatgaact gatccccaaa atgcgtatca ccctgccgct acggctatgg
     5281 cgatactcat tattctggat gccaaatcgg cataaagaca aacttttagg tgagcgacta
     5341 cgactggccc tgcaagaact ggggccggtt tggatcaagt tcgggcaaat gttatcaacc
     5401 cgccgcgatc tttttccacc gcatattgcc gatcagctgg cgttattgca ggacaaagta
     5461 gctccgtttg atggcaagct ggcgaagcag cagattgaag ctgcaatggg cggcttgccg
     5521 gtagaagcgt ggtttgacga ttttgaaatc aagccgctgg cttctgcttc tatcgcccag
     5581 gttcataccg cgcgattgaa atcgaatggt aaagaggtgg tgattaaagt catccgcccg
     5641 gatattttgc cggtcattaa agcggatctg aaacttatct accgtctggc tcgctgggtg
     5701 ccgcgtttgc tgccggatgg tcgccgtctg cgcccaaccg aagtggtgcg cgagtacgaa
     5761 aagaccttga ttgatgaact gaatttgctg cgggaatctg ccaacgccat tcagcttcgg
     5821 cgcaattttg aagacagccc gatgctctac atcccggaag tttaccctga ctattgtagt
     5881 gaagggatga tggtgatgga gcgcatttac ggcattccgg tgtctgatgt tgcggcgctg
     5941 gagaaaaacg gcactaacat gaaattgctg gcggaacgcg gcgtgcaggt gttcttcact
     6001 caggtctttc gcgacagctt tttccatgcc gatatgcacc ctggcaacat cttcgtaagc
     6061 tatgaacacc cggaaaaccc gaaatatatc ggcattgatt gcgggattgt tggctcgcta
     6121 aacaaagaag ataaacgcta tctggcagaa aactttatcg ccttctttaa tcgcgactat
     6181 cgcaaagtgg cagagctaca cgtcgattca ggctgggtgc caccagatac caacgttgaa
     6241 gagttcgaat ttgccattcg tacggtctgt gaacctatct ttgagaaacc gctggccgaa
     6301 atttcgtttg gacatgtact gttaaatctg tttaatacgg cgcgtcgctt caatatggaa
     6361 gtgcagccgc aactggtgtt actccagaaa accctgctct atgtcgaagg ggtaggacgc
     6421 cagctttatc cgcagctcga tttatggaaa acagcgaagc ctttcctgga gtcgtggatt
     6481 aaagatcagg tcggtattcc tgcgctggtg agagcattta aagaaaaagc gccgttctgg
     6541 gtcgaaaaaa tgccagaact gcctgaattg gtttacgaca gtttgcgcca gggcaagtat
     6601 ttacagcaca gtgttgataa gattgcccgc gagcttcagt caaatcatgt acgtcaggga
     6661 caatcgcgtt attttctcgg aattggcgct acgttagtat taagtggcac attcttgttg
     6721 gtcagccgac ctgagtgggg gctgatgccc ggctggttaa tggcaggtgg tctgatcgcc
     6781 tggtttgtcg gttggcgcaa aacacgctga ttttttcatc gctcaaggcg ggccgtgtaa
     6841 cgtataatgc ggctttgttt aatcatcatc taccacagag gaacatgtat gggtggtatc
     6901 agtatttggc agttattgat tattgccgtc atcgttgtac tgctttttgg caccaaaaag
     6961 ctcggctcca tcggttccga tcttggtgcg tcgatcaaag gctttaaaaa agcaatgagc
     7021 gatgatgaac caaagcagga taaaaccagt caggatgctg attttactgc gaaaactatc
     7081 gccgataagc aggcggatac gaatcaggaa caggctaaaa cagaagacgc gaagcgccac
     7141 gataaagagc aggtgtaatc cgtgtttgat atcggtttta gcgaactgct attggtgttc
     7201 atcatcggcc tcgtcgttct ggggccgcaa cgactgcctg tggcggtaaa aacggtagcg
     7261 ggctggattc gcgcgttgcg ttcactggcg acaacggtgc agaacgaact gatccaggag
     7321 ttaaaactcc aggagtttca ggacagtctg aaaaaggttg aaaaggcgag cctcactaac
     7381 ctgacgcccg aactgaaagc gtcgatggat gaactacgcc aggccgcgga gtcgatgaag
     7441 cgttcctacg ttgcaaacga tccagaaaag gcgagcgatg aagcgcacac catccataac
     7501 ccggtggtga aagataatga agctgcgcat gagggcgtaa cgcctgccgc tgcacaaacg
     7561 caggccagtt cgccggaaca gaagccagaa accacgccag agccggtggt aaaacctgct
     7621 gcggacgctg aaccgaaaac cgctgcacct tccccttcgt cgagtgataa accgtaaaca
     7681 tgtctgtaga agatactcaa ccgcttatca cgcatctaat tgagctgcgt aagcgtctgc
     7741 tgaactgcat tatcgcggtg atcgtgatat tcctgtgtct ggtctatttc gccaatgaca
     7801 tctatcacct ggtatccgcg ccattgatca agcagttgcc gcaaggttca acgatgatcg
     7861 ccaccgacgt ggcctcgccg ttctttacgc cgatcaagct gacctttatg gtgtcgctga
     7921 ttctgtcagc gccggtgatt ctctatcagg tgtgggcatt tatcgcccca gcgctgtata
     7981 agcatgaacg tcgcctggtg gtgccgctgc tggtttccag ctctctgctg ttttatatcg
     8041 gcatggcatt cgcctacttt gtggtctttc cgctggcatt tggcttcctt gccaataccg
     8101 cgccggaagg ggtgcaggta tccaccgaca tcgccagcta tttaagcttc gttatggcgc
     8161 tgtttatggc gtttggtgtc tcctttgaag tgccggtggc aattgtgctg ctgtgctgga
     8221 tggggattac ctcgccagaa gacttacgca aaaaacgccc gtatgtgctg gttggtgcat
     8281 tcgttgtcgg gatgttgctg acgccgccgg atgtcttctc gcaaacgcta ttggcgatcc
     8341 cgatgtactg tctgtttgaa atcggtgtct tcttctcacg cttttacgtt ggtaaagggc
     8401 gaaatcggga agaggaaaac gacgctgaag cagaaagcga aaaaactgaa gaataaattc
     8461 aaccgcccgt cagggcggtt gtcatatgga gtacaggatg tttgatatcg gcgttaattt
     8521 gaccagttcg caatttgcga aagaccgtga tgatgttgta gcgcgcgctt ttgacgcggg
     8581 agttaatggg ctactcatca ccggcactaa cctgcgtgaa agccagcagg cgcaaaagct
     8641 ggcgcgtcag tattcgtcct gttggtcaac ggcgggcgta catcctcacg acagcagcca
     8701 gtggcaagct gcgactgaag aagcgattat tgagctggcc gtgcagccag aagtggtggc
     8761 gattggtgaa tgtggtctcg actttaaccg caacttttcg acgccggaag agcaggaacg
     8821 cgcttttgtt gctcagctac gcattgccgc agaattaaac atgccggtat ttatgcactg
     8881 tcgcgatgcc cacgagcggt ttatgacatt gctggagccg tggctggata aactgcctgg
     8941 tgcggttctt cattgcttta ccggcacacg cgaagagatg caggcgtgcg tggcgcgtgg
     9001 aatttatatc ggcattaccg gttgggtttg cgatgaacga cgcgggctgg agctgcggga
     9061 attgttaccg ttgattccgg cggagaaatt gctgatcgaa actgatgcgc cgtatctgct
     9121 cccgcgcgat ttcacgccaa agccatcatc ccggcgcaac gagccagccc atctgcacca
     9181 tattttgcaa cgtattgcgc actggcgtgg agaagatgcc gcatggctgg ctgccaccac
     9241 ggatgccaat gtcaaaacac tgtttgggat tgcgttttag agtttgcgga actcggtgtt
     9301 cttcacactg tgcttaatct ctttattaat aagattaagc aatagcatgg aacgagcctc
     9361 accatcgggt tcggtgaaaa tggcctgaaa gccttcgaac gcgccttcgg taataatcac
     9421 cttatcaccc ggataaggtg ttgccggatc gacaatgtct ttcggtttat ataccgatag
     9481 ctgatgaatc accgccgatg ggactatcgc tggcgacgcg ccaaagcgca cgaagtggct
     9541 gacaccgcgg gtcgcgttga tagtcgtggt atgaatcact tctggatcaa attcgacaaa
     9601 caggtagttg ggaaacaatg gctcactgac tgcagtacgt tttccacgca cgattttttc
     9661 cagggtgatc atcagtgcca ggcaattcac agcctgtctt tcgaggtgtt cctgggcacg
     9721 ttgaagttgc ccgcgcttgc agtacagtaa ataccaggat tgcataatga ctcttatccg
     9781 tttaatcggg gcgcaaggat agcaaaagct ttacgctaag ttaattatat tccccggttt
     9841 gcgttatacc gtcagagttc acgctaattt aacaaattta cagcatcgca aagatgaacg
     9901 ccgtataatg ggcgtagatt aagaggctac aatggacgcc atgaaatata acgatttacg
     9961 cgacttcttg acgctgcttg aacagcaggg tgagctaaaa cgtatcacgc tcccggtgga
    10021 tccgcatctg gaaatcactg aaattgctga ccgcactttg cgtgccggtg ggcctgcgct
    10081 gttgttcgaa aaccctaaag gctactcaat gccggtgctg tgcaacctgt tcggtacgcc
    10141 aaagcgcgtg gcgatgggca tgggacagga agatgtttcg gcgctgcgtg aagttggtaa
    10201 attattggcg tttctgaaag agccggagcc gccaaaaggt ttccgcgacc tgtttgataa
    10261 actgccgcag tttaagcaag tattgaacat gccgacaaag cggctgcgtg gtgtgccctg
    10321 ccaacaaaaa atcgtctctg gcgatgacgt cgatctcaat cgcattccca ttatgacctg
    10381 ctggccggaa gatgccgcac cgctgattac ctgggggctg acagtgacgc gcggcccgca
    10441 taaagagcgg cagaatctgg gcatttatcg ccagcagctg attggtaaaa acaaactgat
    10501 tatgcgctgg ctgtcgcatc gcggcggcgc gctggattat caggagtggt gtgcggcgca
    10561 tccgggcgaa cgtttcccgg tttctgtggc gctgggtgcc gatcccgcca cgattctcgg
    10621 tgcagtcacc cccgttccgg atacactttc agagtatgcg tttgccggat tgctgcgcgg
    10681 taccaagacc gaagtggtga agtgtatctc caatgacctt gaagtgcccg ccagtgcgga
    10741 gattgtgctg gaagggtata tcgaacaagg cgaaactgcg ccggaagggc cgtatggcga
    10801 ccacaccggt tactataacg aagtcgatag tttcccggta tttaccgtga cgcatattac
    10861 ccagcgtgaa gatgcgattt accattccac ctataccggg cgtccgccag atgagcccgc
    10921 ggtactgggc gtggcgttga acgaagtgtt tgtaccgatt ctgcaaaaac agttcccgga
    10981 aattgtcgat ttttacctgc cgccggaagg ctgttcttat cgtctggcgg tagtgacgat
    11041 caaaaaacag tacgccggac acgcgaagcg cgtcatgatg ggcgtctggt cgttcttacg
    11101 ccagtttatg tacactaaat ttgtgatcgt ttgcgatgat gacgtcaacg cacgcgactg
    11161 gaacgatgtg atttgggcga ttaccacccg tatggacccg gcgcgggata ctgttctggt
    11221 agaaaatacg cctattgatt atctggattt tgcctcgcct gtctccgggc tgggttcaaa
    11281 aatggggctg gatgccacga ataaatggcc gggggaaacc cagcgtgaat ggggacgtcc
    11341 catcaaaaaa gatccagatg ttgtcgcgca tattgacgcc atctgggatg aactggctat
    11401 ttttaacaac ggtaaaagcg cctgatgcgc gtttgttttg ccctatttat cgatccgaca
    11461 gagaaagcgc atgacaacct taagctgtaa agtgacctcg gtagaagcta tcacggatac
    11521 cgtatatcgt gtccgcatcg tgccagacgc ggccttttct tttcgtgctg gtcagtattt
    11581 gatggtagtg atggatgagc gcgacaaacg tccgttttca atggcttcga cgccggatga
    11641 aaaagggttt atagagctgc atattggcgc ttctgaaatc aacctttacg cgaaagcagt
    11701 catggaccgc atcctcaaag attatcaaat cgtggtcgat attccctacg gagaagcgtg
    11761 gctgcgcgat gatgaagagc gtccgatgat tttgattgcg ggcggcaccg ggttctccta
    11821 tgcgcgctcg attttattga cggcgttggc gcgtaatccc aatcgcgata tcaccattta
    11881 ctggggtggg cgtgaggagc aacatcttta tgatctctcc gagcttgagg cgctttcgct
    11941 gaagcatcct ggcctgcgtg tggtgccggt ggttgaacaa ccggaagcgg gctggcgtgg
    12001 gcgtactggc accgtgttaa cggcggtctt gcaggatcat ggtacgctgg cagagcatga
    12061 tatctatatt gccggacgtt ttgagatggc gaaaattgct cgtgacctgt tttgcagtga
    12121 gcgtaatgcg cgggaagatc gtctgtttgg cgatgcgttt gcatttatct gagatataaa
    12181 aaaacccgcc cctgacaggc gggaagaacg gcaactaaaa ctttattcag tggcatttag
    12241 atctatgtcg catccggcaa tcggtgcacg atgcctgatg cgacgctggc gcgtcttatc
    12301 aggcctacat tggtgcagga tcggtagacc ggataaggcg ttcacgccgc atccggcaag
    12361 tggttaaacc cgctcaaaca ccgtcgcaat accctgaccc agaccgatac acatcgtcgc
    12421 cagaccaaac tgaacgtctt tgcgttccat cagattcagc agcgtggtgc tgatacgcgc
    12481 accggaacag cccagcggat gacccagcgc gatcgcacca ccattgaggt tgatcttctc
    12541 gtcaatctgc tccattagcc cgagatcttt aatgcacggc aggatctgcg cggcaaaggc
    12601 ttcgttcatc tcaaacacgc cgatatcgct ggcagaaagc cccgcttttt tcagcgccag
    12661 cttcgaggcc ggaaccgggc cgtaacccat aatcgatggg ttacaaccaa cgaccgccat
    12721 cgaacgcaca cgagcgcgcg gcttaagacc taattcatgg gcgcggcttt cactcatcac
    12781 cagcatggca gctgcgccat cggaaagtgc agaagatgtg cccgccgtta ccgtaccgtt
    12841 tactggatca aacgccggac gcagcgtggc gagggcttcc acggtggttt ccggtcgaat
    12901 cacctcgtcg taattaaact gcttcaggac gccgtcggca tcgtgaccac cggtcgggat
    12961 gatttcattt ttaaatgcgc ccgactgcgt ggcggcccag gcgcgggcgt gtgaccgcgc
    13021 ggcaaaggca tcctgcattt cacggctgat accgtgcata cgcgccagca tttctgccgt
    13081 taagcccatc atgcccgccg ctttggcgac attgcggctc aggccggggt gaaaatcgac
    13141 gccgtgactc attggcacat ggcccatatg ctccacgccg ccaaccagac atgcctgcgc
    13201 atcgccagtc atgatcattc gtgctgcgtc atgcagtgcc tgcatggatg aaccacacaa
    13261 gcgattgacg gtaaccgccg ggacagagtg cggcacttct gccagcagcg ccgcgttacg
    13321 ggcgatatta aaaccctgct ccagcgtctg ctgcacacaa ccccagtaaa tatcgtcgag
    13381 ggccgccgct tccagcgccg ggttacgcgc cagcaggcta cgcattaaat gagcggagag
    13441 atcttctgca cgcacgttac gaaaagcacc gcccttcgaa cggcccatcg gggtgcgaat
    13501 tgcatcgaca atgacaacct gttccattgt gactccttaa gccgttttca ggtcgccaac
    13561 cggacgggct ggctcaaccg gaggatagta cggttcgtta tgacgcgctt tattacgcag
    13621 accttccggc acttcataca gcgggccgag gtgctgatat tgctgtgcca tatcgaggta
    13681 ttttgcgcta ccgagggtgt tcagccagcg gaacgcgccg ccgtggaacg gagggaagcc
    13741 caggccgtag accagcgcca tatccgcttc cgccggagtg gcgataatgc cctcttccag
    13801 acagcggacc acttcgttga ccatcgggat catcatgcgg gcgataatct cttcttcgct
    13861 gaaatcgcgc ttcggctggc tcacttctgc cagcaggtct tcaacggcgg cgtcttcttc
    13921 tttcttcggc ttacctttgc tgtcttcttt ataacgccag aaaccgaggc cgttcttctg
    13981 accaaagcgg ttggcatcaa acagcgcgtc gatggcatcg cggtaatctt tctgcatccg
    14041 ctgcgggaag cctgctgcca tgacagcctg agcgtgatgc gcggtatcaa tgcccacgac
    14101 atccagcaga tatgccggac ccataggcca gccaaactgt ttttccatca ctttgtcgat
    14161 cttgcggaaa tccgcgccgt cgcgcagcag ctggctgaaa ccggcgaaat acgggaacag
    14221 cacgcggtta acaaagaagc cggggcagtc gttaaccaca atcggcgtct tgcccatctt
    14281 gctcgcccag gcgacaactt tcgcgatggt ttcgtcggag cttttctcgc cgcgaataat
    14341 ttctaccaac ggcatccggt gtaccgggtt aaaaaagtgc attccgcaga agttttccgg
    14401 gcgttccagc gcgttggcca gttcgctgat aggaatggtt gaagtgttag acgccagtac
    14461 ggtatccggg cgtacttttt gttcggtttc tgccagtacg gcttttttca ctttcgggtt
    14521 ttcgacaacc gcttctacca caacatccac gcggtcaaac ccggcgtagt cgagcgttgg
    14581 gtggatcgtg gagatcacgc cagccagttt cagaccatcg atcttgccgc gttcaagctg
    14641 cttgttcagc agtttcgcgg cttcggtcat gccgagggtt aacgacttgt cgttgatatc
    14701 tttcatgaca accggtacgc ctttccacgc agactggtaa gcaatgccgc cacccatgat
    14761 gcctgcgccc agtacagcgg cctgtttcgg tgtttcaacg tctttggtga gtttcttcgc
    14821 tttgcctttt acatattgat cgttaaggaa aatgccgacc agtgcgcggg cttcgttggt
    14881 gtgcgccagc gggacaaagc ttttgttttc cagatttaat gcttcttcac gaccaaaacg
    14941 ggccgcagct tcaatggttt ttactgcggt gatgggcgcc ggataatgtt tccccgccgt
    15001 ttgtgcgacc atccctttag cgatagtgaa gctcatggtg gcttcaatct tgctcagttt
    15061 tagtggttcc agcttcggct gacgttttgc tttccagtcg aggtcgccgt taatggcctg
    15121 gcgtaaaacc gcctttgcgc cttcaaccag tttttctgct ttgactacgc catccaccag
    15181 accgattttc agcgcctgat ccgcgccgac atctttaccg gcggcaatga tttccagcgc
    15241 actgtcagcg cccagcatac gtggcatacg tacagaaccg ccaaagcccg gcatgatgcc
    15301 cagtttggtt tccggcagac cgatgcgcag atccggcgtc gccagacgat aatcggtcgc
    15361 cagcacgcat tcgcagccac cacccagcgc atagccgttg acggcagcaa tggtcggcac
    15421 cggcagatct tccaggcgat taaacacgct attggcaaag tgcagccact gacttaactg
    15481 ttcttcagga acgaggaacg aggaacgagg aacagggaca aaaattcggt gatatcagca
    15541 ccgacgataa aggctgcttt gttcgaacgc agcagcagcc cttttagatc tgattgctgt
    15601 tccagcacgc cgatggcctc gccgaggctg gcgacggtcg cagtgtcgag tttattaact
    15661 gaacctgggg catcaaatac cagttcggca atgccatctt ccagccagtc aaggtacagg
    15721 gtgtcgcctt tgtaaagcat gtcagtctcc tgaatccgca aggtgatctg gtcgtaccag
    15781 atgcatcgaa gtgtgtattt tgtgttaaaa acatgtaaat aaaagattaa agaaatgccg
    15841 atctgatcac gctcggcaga aatcacgctc tggatgaacg atgtgctaag atgcggagac
    15901 ttaaggtcaa aaaaacagaa gggtaaaaaa tggaatcact ggcctcgctc tataaaaatc
    15961 atatagctac cttacaagaa cggactcgcg atgcgctggc gcgcttcaag ctggatgcat
    16021 tacttattca ctccggcgag ctgttcaacg tttttctcga cgatcatccc tatccgttta
    16081 aagtgaaccc gcaattcaaa gcgtgggtgc cggtaactca ggtgccaaac tgctggttgc
    16141 tggtggatgg cgtgaacaag ccgaaactgt ggttctatct gccggttgat tactggcaca
    16201 acgtcgaacc gctgccgacc tccttctgga ctgaagatgt ggaagtgatc gcactgccga
    16261 aagccgatgg cattggtagc ctgctgcccg ctgcgcgcgg caatatcggt tatatcggtc
    16321 cggtgccgga acgtgcgctg caactgggta ttgaggccag caacatcaac ccgaaagggg
    16381 tgatcgacta cctgcattac taccgctcct tcaaaaccga gtacgaactg gcctgtatgc
    16441 gtgaagcgca gaaaatggcg gtcaacggtc atcgtgcggc agaagaagcg ttccgttctg
    16501 gcatgagcga gttcgatatc aacatcgcct atctgaccgc gaccggtcat cgtgataccg
    16561 acgtacctta cagcaacatt gtggcgctta acgaacacgc ttcggtgctg cattacacca
    16621 aactggatca tcaggcaccg gaagagatgc gcagcttcct gctggatgcc ggggccgaat
    16681 ataacggcta tgcggctgac ctgactcgta cctggtcggc aaaaagcgac aacgactacg
    16741 cacagctggt gaaagacgta aatgatgaac aacttgcgct gatcgctacc atgaaagctg
    16801 gcgtcagcta tgtggattac cacctccagt tccatcagcg catcgccaaa ttgctgcgta
    16861 aacatcaaat catcaccgat atgagtgaag aagcgatggt cgaaaacgat ctcaccggac
    16921 cgtttatgcc gcacggtatc ggccatccgc tgggcctgca ggtgcatgac gtagccggtt
    16981 ttatgcagga tgatagcggt acacacctcg cggcaccggc aaaatatccg tacctgcgct
    17041 gcacccgtat tctccagccg ggcatggtgt taaccatcga accgggtatc tacttcatcg
    17101 aatcgctact ggcaccgtgg cgtgaagggc agttcagcaa gcacttcaac tggcagaaaa
    17161 ttgaagcact gaaaccgttc agcggcattc gtatcgaaga caacgtggtg atccacgaaa
    17221 ataacgtgga aaacatgacc cgggatctga aactggcgtg atggaaagct ggttaattcc
    17281 tgcggcaccg gtcacggtcg ttgaagagat caaaaagagc cgcttcataa cgatgttggc
    17341 gcataccgat ggcgttgagg cggcgaaagc gtttgttgaa tcggtgcggg cagaacaccc
    17401 cgatgcccgc caccattgcg tggcgtgggt ggcgggtgcg ccggatgatt ctcaacagct
    17461 gggtttctct gacgacgggg agccggcggg aacggcaggt aaaccgatgc tcgcccagct
    17521 aatgggcagc ggcatcgggg aaattaccgc tgtggtagtg cgctactacg gcggcatatt
    17581 gcttggcacc ggtgggttag tgaaagcgta tggcggcggc gtgaatcagg cgctgcgcca
    17641 gctaacgacc caacgcaaga cgccattaac cgaatatact ttgcaatgtg aatatagtca
    17701 gttaaccggc attgaagcgt tgctggggca gtgtgacggc aaaattatca acagtgatta
    17761 tcaggcattc gttctgctgc gggtggcgct tccggcggcg aaagtggctg aattttccgc
    17821 aaagctggcg gattttagcc gtggttcatt gcaattgtta gcgattgaag aataatcccc
    17881 acttcatttt gcagaactaa ggaagcggca gagatgcatt ttcgcgccat tacccgaatc
    17941 gttggactac tggtcatctt attttcaggg accatgatta tccctgggct ggtagcactc
    18001 atctaccggg atggagcggg ccgcgctttt acccagacct tttttgtcgc cctcgccatt
    18061 ggctctatgc tgtggtggcc gaaccgcaaa gagaagggcg aactgaaatc ccgtgagggg
    18121 tttctgatag tggtgctgtt ttggaccgtg ctgggtagtg tcggtgcgct cccttttatc
    18181 ttctcggaaa gcccgaacct cacgattacc gatgcgtttt ttgaatcttt ctctggcctg
    18241 accaccaccg gggccactac gctggtgggg ctggattcgc tccctcatgc catcctcttt
    18301 tatcgccaga tgctgcaatg gtttggcggg atggggatca tcgtgttagc ggttgcgata
    18361 ctgcctatcc tcggcgtggg tgggatgcag ctctatcgcg cagaaatgcc cggcccgctg
    18421 aaagataaca aaatgcgccc gcgaattgcg gaaacggcga aaaccctgtg gttgatttat
    18481 gtcttgctga ccgtcgcctg tgcgctggcg ttgtggtttg ccggaatgga tgcctttgat
    18541 gccatcggcc atagctttgc gactatcgct attggcggct tctcgacaca tgatgccagt
    18601 atcggttatt tcgacagtcc gactattaac actatcattg ctatcttcct gctgatctcc
    18661 ggttgtaact acggtctgca cttttcactg ttaagtgggc gtagtctgaa ggtttattgg
    18721 cgcgatccgg aatttcgcat gtttatcggc gtacagttta cgctggtggt tatttgtaca
    18781 ctcgtactgt ggtttcataa tgtctacagt tcggcgctga tgacaattaa ccaggcgttt
    18841 ttccaggtgg tatcgatggc gacaaccgcc gggtttacga ctgacagcat tgcccgctgg
    18901 ccgctctttt tgccggtact gcttttatgt tcagctttta tcggcggttg cgccgggtca
    18961 acgggcggtg gcctgaaagt gatccgcatc ctgctgctgt ttaagcaggg gaaccgtgag
    19021 ctgaaacgac tggtgcatcc gaacgccgtc tatagcatta agctggggaa tcgcgcactg
    19081 ccggaacgta tcctcgaagc cgtttgggga tttttctccg cctatgcatt ggtgtttatt
    19141 gtcagtatgc tggcgattat cgccacgggc gtggatgatt tttctgcctt tgcctcagtt
    19201 gttgcgacat tgaataacct ggggccaggg cttggcgtgg ttgctgataa ctttaccagt
    19261 atgaacccgg tggctaaatg gatcctgatt gccaacatgc tgtttggtcg tctcgaggtc
    19321 tttacattgc tggtgctctt taccccgact ttctggcgtg aatgatggag taatacgtga
    19381 aaacattaat tcttttctca acaagggacg gacaaacgcg cgagattgcc tcctacctgg
    19441 cttcggaact gaaagaactg gggatccaga cggatgtcgc caatgtgcac cgcattgaag
    19501 aaccacaatg ggaaaactat gaccgtgtgg tcattggtgc ttctattcgc tatggtcact
    19561 accactctgc gttccaggaa tttgtcaaaa aacatgcgac gcgactgaat tcgatgccga
    19621 gcgcctttta ttccgtaaac ctggtggcgc gcaaaccgga gaagcgtact ccacagacca
    19681 acagctacgc gcgcaagttt ctgatgagtt cacaatggcg tcccgatcgc tgcgcggtca
    19741 ttgccggggc gctgcgttac ccacgttatc gctggtacga ccgttttatg attaagctga
    19801 ttatgaagat gtcaggcggt gaaacggata cgcgcaaaga agttgtctat accgattggg
    19861 agcaggtggc gaatttcgcc cgagaaatcg cccagttaac caacaaaccg acgctgaaat
    19921 aagcataaag aataaaaagt gcgcggtcag aaaattattt taaatttcct cttgtcaggc
    19981 cggaataact ccctataatg cgccaccact gacacggaac aacggcaaac acgccgccgg
    20041 gtcagcgggg ttctcctgag aatctcaaca g
//
LOCUS       DAZPEB010000058        19878 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZPEB010000058 DAZPEB010000000
VERSION     DAZPEB010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19878
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.059"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..833
                     /gene="eutC"
                     /locus_tag="ACHURJ_001843"
     CDS             <1..833
                     /gene="eutC"
                     /locus_tag="ACHURJ_001843"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFR1280814.1"
                     /translation="TAPALSEAKCTTTTCAAPVTSESCALDLGSAEAKAWIGVENPHR
                     ADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRSKDTVLKEVPEEWVKAQGLL
                     EVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPDVQVVISDGLSTDAITVNYE
                     EILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILGAKVVILLVGERPGLGQSES
                     LSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVIVDLAKRMLEQKASGINMTR
                     "
     gene            843..1502
                     /gene="eutL"
                     /locus_tag="ACHURJ_001844"
     CDS             843..1502
                     /gene="eutL"
                     /locus_tag="ACHURJ_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFR1280815.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            1515..2015
                     /gene="eutK"
                     /locus_tag="ACHURJ_001845"
     CDS             1515..2015
                     /gene="eutK"
                     /locus_tag="ACHURJ_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFR1280816.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAAGRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVREPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            2061..3113
                     /gene="eutR"
                     /locus_tag="ACHURJ_001846"
     CDS             2061..3113
                     /gene="eutR"
                     /locus_tag="ACHURJ_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFR1280817.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRLANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFVTDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(3119..4018)
                     /gene="hemF"
                     /locus_tag="ACHURJ_001847"
     CDS             complement(3119..4018)
                     /gene="hemF"
                     /locus_tag="ACHURJ_001847"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFR1280818.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(4022..4891)
                     /gene="amiA"
                     /locus_tag="ACHURJ_001848"
     CDS             complement(4022..4891)
                     /gene="amiA"
                     /locus_tag="ACHURJ_001848"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFR1280819.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            5105..5530
                     /locus_tag="ACHURJ_001849"
     CDS             5105..5530
                     /locus_tag="ACHURJ_001849"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFR1280820.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADMLSLGKRLIEDEEY"
     gene            5517..5966
                     /locus_tag="ACHURJ_001850"
     CDS             5517..5966
                     /locus_tag="ACHURJ_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFR1280821.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFIIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            6173..6748
                     /locus_tag="ACHURJ_001851"
     CDS             6173..6748
                     /locus_tag="ACHURJ_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFR1280822.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFRGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            6844..7743
                     /gene="yfeX"
                     /locus_tag="ACHURJ_001852"
     CDS             6844..7743
                     /gene="yfeX"
                     /locus_tag="ACHURJ_001852"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFR1280823.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(7801..9105)
                     /gene="pbp4b"
                     /locus_tag="ACHURJ_001853"
                     /pseudo
     CDS             complement(7801..9105)
                     /gene="pbp4b"
                     /locus_tag="ACHURJ_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
     gene            complement(9110..10534)
                     /gene="murP"
                     /locus_tag="ACHURJ_001854"
     CDS             complement(9110..10534)
                     /gene="murP"
                     /locus_tag="ACHURJ_001854"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFR1280824.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKSVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRSQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(10538..11434)
                     /gene="murQ"
                     /locus_tag="ACHURJ_001855"
     CDS             complement(10538..11434)
                     /gene="murQ"
                     /locus_tag="ACHURJ_001855"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFR1280825.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            11598..12455
                     /gene="murR"
                     /locus_tag="ACHURJ_001856"
     CDS             11598..12455
                     /gene="murR"
                     /locus_tag="ACHURJ_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HFR1280826.1"
                     /translation="MLYLTKISNAGSEFTENEQKIADFLQANVSELQSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNTTALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCVEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVASLKMIQR
                     SSELTQRLK"
     gene            12584..13375
                     /gene="ucpA"
                     /locus_tag="ACHURJ_001857"
     CDS             12584..13375
                     /gene="ucpA"
                     /locus_tag="ACHURJ_001857"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFR1280827.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            13679..14695
                     /gene="cysP"
                     /locus_tag="ACHURJ_001858"
     CDS             13679..14695
                     /gene="cysP"
                     /locus_tag="ACHURJ_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFR1280828.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKTDVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            14695..15528
                     /gene="cysT"
                     /locus_tag="ACHURJ_001859"
     CDS             14695..15528
                     /gene="cysT"
                     /locus_tag="ACHURJ_001859"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFR1280829.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            15528..16403
                     /gene="cysW"
                     /locus_tag="ACHURJ_001860"
     CDS             15528..16403
                     /gene="cysW"
                     /locus_tag="ACHURJ_001860"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFR1280830.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            16393..17490
                     /gene="cysA"
                     /locus_tag="ACHURJ_001861"
     CDS             16393..17490
                     /gene="cysA"
                     /locus_tag="ACHURJ_001861"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFR1280831.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            17624..18535
                     /gene="cysM"
                     /locus_tag="ACHURJ_001862"
     CDS             17624..18535
                     /gene="cysM"
                     /locus_tag="ACHURJ_001862"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFR1280832.1"
                     /translation="MSTLEQTIGNTPLIKLQRMGPNNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(18538..18906)
                     /locus_tag="ACHURJ_001863"
     CDS             complement(18538..18906)
                     /locus_tag="ACHURJ_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFR1280833.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTAHEEARINAMLEGLSQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            19011..19862
                     /gene="pdxK"
                     /locus_tag="ACHURJ_001864"
                     /gene_synonym="thiJ"
     CDS             19011..19862
                     /gene="pdxK"
                     /locus_tag="ACHURJ_001864"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFR1280834.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
BASE COUNT         4577 a         5044 c         5607 g         4650 t
ORIGIN      
        1 aaacggcccc cgcgctgtca gaagcaaagt gcaccaccac cacctgtgcg gcaccggtga
       61 cctcggaaag ctgcgcgctg gatttaggtt ctgcggaagc aaaagcgtgg attggtgttg
      121 aaaacccgca tcgcgcagac gtattaacag aactgcgtcg cagcaccgtg gcccgcgttt
      181 gtaccggtcg tgccggtccg cgtccgcgta cccaggcgtt gctgcgtttc ctggccgatc
      241 actctcgttc gaaagatacc gtactgaaag aagtgccgga agagtgggtg aaagcgcagg
      301 gcttgctgga agtacgctcc gaaattagcg acaaaaatct ctacctgact cgcccggata
      361 tgggccgccg cctgtgtgca gaagctgttg aagcgctgaa agcacagtgt gttgctaatc
      421 cggacgtaca ggtcgttatt tctgatggcc tgtcaactga tgccatcacc gtgaactacg
      481 aagagatcct gccgccgctg atggcgggcc tgaaacaggc cgggctgaaa gtcggcacgc
      541 cattctttgt tcgttatggt cgcgtgaaga ttgaagatca gatcggtgag atcctcggcg
      601 cgaaagtggt gatcctgctg gtgggggaac gtccggggtt gggtcagtca gaaagcctct
      661 cctgttacgc cgtttactcg ccgcgtatgg cgaccaccgt tgaggccgat cgcacctgca
      721 tctctaacat tcaccagggc ggcacgccgc cggttgaagc cgcagccgtc attgtggatt
      781 tggcgaaacg tatgctggag cagaaagcat ccggcatcaa catgacccga taaggaggca
      841 tcatgccagc tttagatttg attcgaccgt cggtaaccgc catgcgggtg attgcctccg
      901 ttaacgctga atttgcgcgt gaactaaaat tgccgccgca tattcgtagc ctcgggctga
      961 tttctgctga ttccgatgac gtcacctaca ttgcggctga cgaagcgacc aagcaggcga
     1021 tggtcgaagt ggtatatggc cgctcgctgt atgcgggagc agcacacgga ccgtcaccga
     1081 ctgccggtga agtgctgatt atgcttggtg ggccaaaccc ggcggaagtg cgtgctggtc
     1141 tggatgcgat ggttgcgcat attgaaaatg gcgctgcttt ccagtgggct aatgacgcag
     1201 aaaacaccgc attcctggca catgtagttt cgcgtaccgg ttcttatctc tcatcaaccg
     1261 ccgggatcac gcttggcgat ccgatggcgt atctggtggc accgccgctg gaagcgacct
     1321 acggcattga tgcagcgttg aaatctgccg acgtacagct ggtgacctat gtcccgccac
     1381 cgtctgaaac caactactcg gcagcatttt taaccggtag ccaggccgcg tgtaaagcag
     1441 cctgtaacgc ctttaccgat gcagtgctgg aaatcgcgcg taatccaatc cagcgtgcgt
     1501 aacggaggtt gccgatgatc aatgcactgg gattgctgga agtggacggc atggtcgccg
     1561 cgatagatgc agcggatgcc atgctcaaag cagctaacgt tcgtctgctc agtcacgaag
     1621 tgcttgaccc tggtcgctta acgctggtgg tggaaggcga tctggcggcg ggtcgtgcgg
     1681 cgctggacgc aggttgtgct gccgcgatgc gtaccgggcg tgtcatcagc cgcaaggaga
     1741 tcggtcggcc agacgatgac acccagtggc tggttactgg ctttaaccgc cagccgaagc
     1801 aacccgtaag ggaacccgac gcgccagtta tcgtcgcgga atctgctgac gagttgttgg
     1861 cgctgttaac atcagtacgt cagggaatga cggcaggaga agtggctgcc cactttggct
     1921 ggccgctgga aaaagccaga aatgcgctcg aacagctctt ttctgccggg acgttacgta
     1981 aacgcagtag tcgttatcgt ctcaagcccc attaacctgt cggaggtgct gggtgtccta
     2041 caacacccgg cattaacatc atgaaaaaga cccgtacagc caatttgcac catctttatc
     2101 atgaaccctt acccgaaaac ctgaagctca cgccgaaggt cgaagtggat aatgttcatc
     2161 aacgacagac aacggatgtc tatgaacatg ctttaacgat taccgcctgg cagcagattt
     2221 acgatcagct gcatccgggc aagtttcatg gtgaatttac ggaaattcta ctcgatgata
     2281 ttcaggtttt tcgtgaatac accggtctgg cgctgcgtca gtcgtgcctg gtctggccga
     2341 actcgttctg gtttggcatt ccggcgacgc gcggtgagca gggatttatc ggttcgcaat
     2401 gtctgggaag cgcggaaatc gccacccgcc ctggtggcac tgaatttgaa ctgagcacgc
     2461 cggatgatta cacgatcctg ggcgtggtgc tttctgaaga tgtcatcacc cggctggcta
     2521 actttttgca taacccggat cgggtattac atatgctgcg tagccagtcg gcgctggaag
     2581 tgaaagagca gcataaagcc gcgctgtggg gctttgtcca acaggcgctg gcgacgtttt
     2641 gcgagaaccc ggaaaatctc catcagcctg cagtgcgaaa agtgctgggg gataatttgc
     2701 taatggcgat gggggcgatg ctggaagacg cgcaaccaat ggtgacggcg gaaagcatca
     2761 gtcatcagag ttaccgtaga ttactttccc gcgcccgtga atatgtgctg gaaaatatgt
     2821 ctgagccggt gacggtgctg gacttgtgta atcaactgca tgtcagtcgc cgcacgctac
     2881 aaaacgcgtt tcacgctatt ttaggcattg gcccaaacgc gtggctgaaa cgcattcgcc
     2941 tgaacgccgt acgccgcgaa ctgataagcc cgtggtcgca aagcacaacg gtaaaagacg
     3001 ccgccatgca gtggggattc tggcatctgg ggcaatttgt cacggattac cagcagctgt
     3061 ttgccgagaa gccgtcgttg acgttgcatc agcggatgcg ggaatggggg tgagggagtt
     3121 acacccaatc cctgacctta ataaactcac ttaacgccgc ttctgggctg ccatcttttg
     3181 gctgataatc atactcccag cgtaccagtg gcggcattga catcaggatc gactcggtgc
     3241 gcccgccagt ttgcaggcca aacagagtgc cgcgatccca gaccagattg aactcgacat
     3301 aacgaccgcg acggtagagc tgaaaattgc gctcgcgctc gccgtaggcc attgctttac
     3361 gtcgctctac aattggtaaa taagcgtcgg tgtagccttt gcctaccgcc tgcataaagg
     3421 caaaacagtg gtcgaaatct ggcgtgttca gatcatcaaa gaacagcccg ccaataccgc
     3481 gctgttcgtt gcgatgtttg aggtagaagt aatcgtcgca ccactttttg taacggggat
     3541 aaacgtcttc accaaatggc aggcacaggt cacgggcggt gcggtgccag tgaatggcgt
     3601 cttcttcaaa accatagaaa ggggttaaat cgaagccgcc gccaaaccac cagacgggct
     3661 ctgcacccgg tttttcggca ataaaaaaac gcacattcgc gtggctggtg ggaacatacg
     3721 ggttatgcgg atgcactacc agtgaaacgc ccatcgcctc gaaactgcgc ccggcaagtt
     3781 ccgggcgatg agcggtggcg gaagcaggca tcgcctcgcc gtggacgtga gaaaagttga
     3841 cgcctgcctg ttcgaaaaca ccaccattac gcaacacccg actacgcccg ccgccgccag
     3901 cttcgcgctg ccaactatct tcgacaaatt ctgcgccatc gacggcggtc agctgctgac
     3961 aaatcgtatc ctgaaggttg agcagaaact gtttaacctg gtgtgcgtcg ggtttcataa
     4021 cttatcgctt tttcgaatgt gctttctggt tgtcgaacca gtggaaataa ctgatcacgc
     4081 cttcagcaat cgctgtggcg attttctgac gaaacgccgc cgtgcctaac agccgctctt
     4141 cttccgggtt ggtgataaac gaggtttcca ccagcaccga aggaaccgac ggcgatttca
     4201 acaccacaaa cgcggcttgt tcggtgttgc ggctgtgcag tttatgcacc ggcttaatct
     4261 ttttcagaat atgcgagcca agcgtcaggc tgtttttaat agtatcggtt tgcaccagat
     4321 caaacagcac ttgctgcaat aggtgatcct tgtcagtcgc ctttttaccg gcaacttcat
     4381 cggcgcggtt ttcgcgttca gacaggtatt tcgccattgc gctacttgcc ccacggttag
     4441 agagggcaaa taccgaagca ccggcagctt tcgggttggt aaagccatcg gcatgaattg
     4501 acataaacag atctgcgcca tgtttatggg cgatttcaac gcgatcgtaa agtgggataa
     4561 acgtatcgcc agaacgcgtt aaacgcgcat caatcccatg attacgcaaa atggaacgga
     4621 cgtttttagc aatcgccagc accacatgtt tttctttcga accgttgcgt ccgatcgctc
     4681 cggtatcaat tccgccgtgg cctggatcga gaacaacgac acgtttgccg ccagattttt
     4741 tggctttcgg cttgctgtgt ccgttgctgg tttttaaagg ttcgtctttg gcgatggctt
     4801 gcgacattcc tgacaacgtc agggcagcca aaccggcttt cagcacctgg cggcgcgaag
     4861 tgagtgtttt tagtggttta aaagtgctca tacggcctga gttgtaataa taaagttcca
     4921 gatgttatat cgtatcgcgt aatccgttac gacggtttga tgttgagaat tgttttactt
     4981 ttcatttcaa tagttgacaa ctccccatta tgccactttt tcaaccgatt ttcgtcagat
     5041 attggctaat tctaccgttc acgccataat cagcctttta acccgcaaaa aggtaacgaa
     5101 taccatggag atacgcgtat ttcgccagga agatttcgaa gaggtcatca ccctttggga
     5161 gcgttgcgac ttgctgcgtc cgtggaacga tccggaaatg gacatcgagc gtaagatgaa
     5221 ccatgacgtc agtttgtttt tggtcgctga ggtgaacggt gaagtggtcg gaacggtgat
     5281 gggcggttat gacgggcatc gcgggtctgc ttattatctt ggcgtgcatc cagagtttcg
     5341 tgggcgtggg attgccaatg cgttgcttaa tcggctggag aaaaagctga ttgctcgtgg
     5401 ctgcccgaaa attcagatca atgtgccgga agataacgac atggtgctcg gaatgtatga
     5461 acgcctggga tatgaacacg ccgacatgct gagtctgggt aagcgtttga ttgaagatga
     5521 agagtactga gttccatcct gtccattatg atgcgcacgg acgcctgcgt ttacccttgc
     5581 tcttctggct tgtgctattg cttcaggcgc gaacctgggt gctgtttatc attgccggtg
     5641 cgtcgcgcga gcagggtact gcgctgctaa atctgtttta tcccgatcac gataatttct
     5701 ggctggggtt gattcccggt attccggcgg tgctggcgtt tttgttgagc ggtcggcggg
     5761 cttcgtttcc tcgcacctgg catgtgctct attttctgtt gctgttggcg caggtggttt
     5821 tactttgctg gcaaccgtgg ctgtggctga acggtgaatc cgttagcggt atcggtctgg
     5881 cgctggtagt ggcggatatt gtggcgttaa tctggctgct gaccaatcga cgtttacgcg
     5941 cttgttttaa tgaagaaaaa gaataacgca gaggtgcgct ttgtttatgc cggatgcggc
     6001 gtgaacgcct tatccggcct acaaaacatc gaaaattcaa tatattgcag gagaaagtag
     6061 gcctgataag cgtagcgcat caggcaattt tgtgtttgtc atcaaccttt aacggcactt
     6121 tttggtgaat ttgcactcca agcaacgtta ttgaataacc aaaggaagtg acatgaaatc
     6181 gctgcgttta atgttatgcg ctatgccgtt gatgctgacc ggctgttcca cgatgtcgtc
     6241 agttaactgg tcggctgcta acccgtggaa ctggtttgga tcgtccacca aagtgagcga
     6301 gcagggtgtg ggtgaattaa cggcgtccac accactgcaa gaacaagcca ttgctgatgc
     6361 gcttgatggc gattatcgcc tgcgcagcgg aatgaaaacc gcgaacggca acgtggtgcg
     6421 cttttttgaa gtgatgaaag gcgacaacgt ggcgatggtg attaacggcg atcaaggcac
     6481 gatcagtcgc attgacgtgc tggatagcga tattcctgct gacaccggtg ttaaaatcgg
     6541 tacaccgttt agcgaccttt acagcaaagc atttggcaat tgccaaaaag ctgatggtga
     6601 tgataatcgt gccgtcgaat gtaaagccga aggcagtcaa catattagct accagttcag
     6661 aggggaatgg cgtggtcctg aagggttaat gccttcggac gataccctga aaaactggaa
     6721 agtcagtaaa attatctggc ggcgttaatt tgcgtctgaa caaaccgccg ccgcgaaaaa
     6781 acacgtaaaa tagcgctcaa caatgccacg gattgcgtgg cattcttatt ttcaggagga
     6841 acaatgtctc aggttcagag tggcattttg ccagaacatt gccgcgcggc gatttggatc
     6901 gaagccaacg tgaaagggga agttgacgcc ctgcgtgcgg ccagtaaaac atttgccgac
     6961 aaactggcaa cttttgaagc gaaattcccg gacgcgcatc ttggtgcggt ggttgccttt
     7021 ggtaacaaca cctggcgcgc tctgagcggc ggcgttgggg cagaagagct gaaagatttt
     7081 ccgggctacg gtaaaggcct tgcgccgacg acccagttcg atgtgttgat ccacattctt
     7141 tctctgcgtc acgacgtaaa cttctctgtc gctcaggcgg cgatggaagc ctttggtgac
     7201 tgcattgaag tgaaagaaga gatccacggc ttccgttggg tggaagagcg tgacctgagc
     7261 ggctttgttg acggtacaga aaacccggcg ggtgaagaga cgcgccgcga agtggcggtt
     7321 atcaaagacg gcgtggatgc cggcggcagc tatgtgtttg tgcagcgttg ggagcacaac
     7381 ctgaagcagc tcaaccggat gagcgttcac gatcaggaga tgatgatcgg gcgcaccaaa
     7441 gaggccaacg aagagattga cggcgacgaa cgtccggaaa cctctcacct cacccgcgtt
     7501 gatctgaaag aagatggcaa agggctgaag attgttcgcc agagcctgcc gtacggcacc
     7561 gccagtggca ctcacggtct gtacttctgc gcctactgcg cgcgtctgca taacattgag
     7621 cagcaactgc tgagcatgtt tggcgatacc gatggtaagc gtgatgcgat gttgcgcttc
     7681 accaaaccgg taaccggcgg ctattacttc gcgccgtcgc tggacaagct gatggcgctg
     7741 taagcgtttc tgattctatg attaaaggcc agccagtaac aaaactgact ggccttttta
     7801 ttacttttgc ttcaacgccg catacacctg atcaactacc caaccataag ttgcaatcgg
     7861 taactgaccg ctttcgaaca tattgggatt tttttgtgga tcggcaactg gcgaatgggg
     7921 cttgttgctt aacatcacaa tcgccatatg attgaccgga tcgataacgg ttactgtccc
     7981 ggtccagcca gtgtgaccgt aggtttgcgg gcttgccagc gtgccaaacg tcggcgtcat
     8041 ggtggcatta ccattcacgc gccagccgag gccaaaagtg gcatcttcct tagagctggt
     8101 ggtgaacatc ttcaccgttt ccgcactgaa cagctgcaca tcgccatagc ccccgccgtt
     8161 cagcatcgtt tgcattaaca ccgcaatatc gccggtattg gaaaacaaac ctgcgtgccc
     8221 ggaaacgccg cccatcgaat aaaaggcttt ttcatcgtgc acctgacccc agagagtgga
     8281 ggtgcggata ttcgggaaat ggatcacgcc atcgcgggta ttgccgttca attccgtggc
     8341 ggcaatttgt tgcggtttaa agcctttcag cagcgggtta aataccgtat gtgtcaggcc
     8401 gagcgggcga taaatcgact cttcaacata gcggtcaagc ggctgaccgg taaccgactc
     8461 gacgataaat ccaagcagca tataatcgac atcgctgtag atatgtttgc tgccgggctg
     8521 atattccagc ggcgtgcgct tgatcatctc cagcgtctgg cctttatcct aggaatataa
     8581 cacgcccgcg accgctttat tcgggtattg cggatctgcc gggaaaccgc cgctgtgatg
     8641 cagcaggtca gaaatccgca gggtgttttt gcctttaatg gtgtcatttg gactatcggc
     8701 aaaccccgga atatatttcg caatccgatc gtcaggatgc agtttgcctt cgctcatcag
     8761 cttttgcagg gcgaagttcg tggcgtacat tttggtgttt gaggccaggt catacagcgt
     8821 cccggtggtg gctttgacag gctgttccat caacacgctg ccatcgtact ttttcgccgc
     8881 tccccaggct ttgcgataca caatctgatt atctttaatg atcagcaggt ttacgccggg
     8941 ataaccggca tcaacttgct ggctaatcca gcgatccatc tggttaagcc gtttgacgtt
     9001 aaaccctgct ttctctggcg agctttctgt cagaacaggg tattttgcgg cgctgacgct
     9061 acaactaaca gccagcagag aaagatagag cattgtccgt ttcatgatat cagtccagat
     9121 tgacgttacg gcagccaaag agcgtggtga aaataaaccc gcaaacccat gccaccagaa
     9181 tcccgccagc ataaaccgcc atggccggaa ggatgccttg tgcggaagtc atcagcggca
     9241 gcgctaccag accagacggc ccaaaggcgc tgtttaagcc catcggcaga ccccaccagg
     9301 ctatcaggcc gataaacaaa ccgcccgccg caccgcctaa acaggcggtg acaaacggct
     9361 tcatgcgggg cagggtgaca ccgtaaatca gcggttcacc aacgcccagc aggccgggaa
     9421 taatcgcccc gcgtacctga ctgcgtagcg cactgtgcgg ttgcgaccgc cagtagagtg
     9481 ccagcgccgc gcccacctgg cccgcgcctg ccattgaaag gatgggaaat aagctgttga
     9541 atccctggct gtccattaac gcgaggtaaa caggaataaa gccctgatgc acgccaaata
     9601 ccacggcgat caggaacagc ccggctaaaa ccgcacaacc gaacggatta ctgttcaggt
     9661 gcataaacag ccatgacata ccttcgaata accagccacc cagcggcata atgatcaggt
     9721 acgcaagcgt agcggtgatc agcagggtga ttaacgaggt cagcagcatg tcgagatcgt
     9781 ccggcataaa gcggcgcacc atgccttcaa tgcgggcgca ggcccacgcg gcaatcagca
     9841 cgccgataat attgccgcgc ggatcgatgg gcagaccaaa gaaatcgtga aaaccggcgt
     9901 agtaaccggt ggttgcagcg gggttataac cgagcaaaaa gagtgcggca ataattgcgc
     9961 cattgacgcc cgtgccgcca aatgcctggg cggcgttata gcccaccaga atcaccagga
    10021 aggtgaacaa acctttgctg aacaccttca taaaattcag cgcgtcgggt agcgtgccct
    10081 gagcatctgc cggaacgtgc atcaccgtgg caattaacgt cgctatcccc agcagcagac
    10141 cggcggcaat aaaaccgggg atcagcggcg tgaagatggt ggcgaatttg gcgagaaatt
    10201 gttgcacgcc ggaggtttgt ttggctttta actgacgttt gttctgggcg gcgatttctg
    10261 cggcatcctg taccggtgcc tcgcccagca gctcgctcat cgcttttgcg gcacgatgcg
    10321 ctttaccagg tccaaaaacg acctgcacct ggtcgctggt caaaatgacg ctcttcacgc
    10381 cttccagcgt tttgatattg ggatcaacca gtgaactgtc atgtacaccc agacgcaggc
    10441 gcgtcataca gttaccacaa ctggcgatat ttcccggtcc gccgacacgg gtaagaatgg
    10501 tgttcagaag ttcactgctg atctctttgg ccatgggtta ttccttgtct aaaacctgac
    10561 gaataaagcc gccgtgttga tccaggcgtt ttttagcttc tgcggcatcg agatttttca
    10621 gcaccatcac aatggccgtt ttacagttgc gctcgcaggc aattaacgcc gcttccgctt
    10681 gttcggcatt acatccggtg gcgtttttaa cgatattgac ctggcgcaca tgcagttttt
    10741 cgttggtggc gaccacatcg accataaggt tgccgaacac tttgccggat ttaatcatca
    10801 gcccggtgga aagcatattg agcaccagtt tctgcgctgt acctgctttc atccgcgaag
    10861 aaccggtaac aacttcggca cctacaatcg gtgtaatggc aaactcagcg gtggttgaaa
    10921 cggcgctccc cggattacag gaaattccca ctgtgcggca gccgagctgg cgtgcgtatt
    10981 ccagtccggc aatcacatac ggcgtgcgac cgctggcagc aatgccaacc accacatcct
    11041 gtgccgttaa attaatattt ttcagatcat taacaccgcc ttcccggcta tcttccgcgc
    11101 cttccaccgc gtgctgaatg gcatattcgc cgccagcaat caaaccaacc accagacccg
    11161 gtttcacgcc gtaggtgggc ggacattcgc tggcatccag aatccccaga cgaccggatg
    11221 ttcccgcacc gaggtaaatc agacgcccgc cgccgcttac ctgggcgtgg ataacatcga
    11281 tcgccgcggc gatatccggc agtacgcgct caacggcaag cggtacggtt ttatcttcat
    11341 cgttgataat ccggcacatt tccagcgttg atacgcggtc aatttcagcc gaggcggcgt
    11401 tcgagccttc agtaatcatc ttttcaagtt gcatcggtgg tgaccttact atttacgatc
    11461 aaataattgc agatgtatgg aatcatatat cctgaattga cagacttaaa ggaatcattg
    11521 agtctacact cgcagagtgt gaagagcgtc ataagaactt aagcggaatt tatgctgcca
    11581 gcccaggaga gtggaagatg ttgtacctga cgaaaataag caatgcgggg agtgaattca
    11641 ccgaaaacga gcaaaaaatc gctgactttc tgcaggccaa cgtgagtgag ctgcaatccg
    11701 tttcttcacg gcagatggcg aaacagctcg gcattagcca gtcgagcata gtgaaatttg
    11761 cccaaaaact cggcgcgcaa ggatttactg aattgcgtat ggcgttaata ggcgaataca
    11821 gtgctagccg ggagaaaacg aataccacgg cgctgcacct gcacagttcg attaccagtg
    11881 atgattcgct ggaagtgatt gcccgcaaat tgaatcgcga aaaagagctg gcgctggagc
    11941 aaacctgtgc gttgttcgac tacgcgcggc tgcaaaaaat cattgaggtc atcagtaaag
    12001 caccgtttat ccagataacg ggcctgggcg gatcggcgct ggtggggcgc gatctgtcgt
    12061 tcaaactgat gaaaattggc tatcgcgtgg cctgcgaagc cgacacgcac gtacaagcca
    12121 ccgtttccca ggcgttgaaa aaaggcgatg tacaaatcgc tatctcttac agcggtagta
    12181 aaaaagagat tgtcttgtgc gtagaagcgg cacgaaagca gggggcaacg gtgattgcca
    12241 ttacttctct ggctgactct ccgctgcggc ggctggcgca tttcacgctc gataccgttt
    12301 ccggtgaaac cgaatggcgt agctcgtcga tgtctacccg cacggcgcaa aattctgtta
    12361 ccgatttact gtttgttggt cttgtgcaac tcaatgatgt ggcgtcattg aaaatgattc
    12421 agcgcagcag tgagctaaca caacgcctga aatgatgcat aaagcagcga ctggattgag
    12481 attttcctga attagtgagc tgatccgcag caatattttg tttatcctgt attttcagag
    12541 ggaatggagt gtaacgctct gtattaacaa ggagagcatt aaaatgggta aactcacggg
    12601 caagacagca ctgattacgg gcgcattgca gggaattggc gaaggaattg ccagaacttt
    12661 tgcacgtcat ggcgcgaacc taatcttgct ggatatctcc cctgagatcg aaaagctggc
    12721 ggacgaactg tgtggtcgtg gtcatcgctg tacggcggtt gtcgccgatg tgcgtgaccc
    12781 ggcgtcggta gccgcagcta tcaaacgcgc gaaggaaaaa gaagggcgca ttgatatcct
    12841 ggtgaataac gcaggcgttt gtcgtctggg cagtttcctc gatatgagcg atgacgatcg
    12901 cgatttccat attgatatca atattaaagg cgtatggaac gtcacgaagg cggtgctgcc
    12961 ggagatgatt gcgcgcaaag atggtcgcat tgtgatgatg tcttcagtca ctggtgatat
    13021 ggtggccgat cctggcgaaa cggcgtacgc cttaacgaaa gcggcgattg ttggcctgac
    13081 aaaatcgctg gcggtggagt acgcgcagtc tggtattcgc gttaacgcca tttgcccggg
    13141 atacgtgcgc acaccaatgg cggaaagcat tgcccgccag tcgaacccgg aagatccaga
    13201 gtcggtgctg actgaaatgg cgaaagcaat cccgatgcgt cgcctcgccg atccgctgga
    13261 agtcggcgaa ctggcggcct tcctcgcatc ggatgaatcc agctatttaa ccggtacaca
    13321 gaatgtgatt gatggcggca gcacactgcc ggagacggtt agcgtcggta tctgattcac
    13381 ctctgtttcc tccctgcatg tgtggggagg atttcgtctt gaactaagtt caccaggcta
    13441 ttttatttgt cattttggcc ccgggcagtg ctcgaaatac tcacgtacta tgtgtacgct
    13501 ccggtttctc cgcgctgtcc gtgtccaaac tgcccgcaac aattacgcct gttgaaccaa
    13561 gttcttattc cctttttaac ttccaaatca ccaaacggta tataaaaccg ttactccttt
    13621 cacgtccgtt ataaatatga tggctattag aaagtcatta aatttataag ggtgcgcaat
    13681 ggccgttaac ttactgaaaa agaactcact cgcgctggtc gcttctctgc tgctggcggg
    13741 ccatgtacag gcaacggaac tgctgaacag ttcttatgac gtctcccgcg agctgtttgc
    13801 cgccctgaat ccgccgtttg aacagcaatg ggcaaaagat aacggcggcg acaaactgac
    13861 gataaaacaa tctcatgccg ggtcatcaaa acaggcgctg gcaattttgc agggcttaaa
    13921 aaccgacgtt gtcacttata accaggtgac cgacgtacaa attctgcacg acaaaggcaa
    13981 gctgatcccg gctgactggc agtcgcgcct gccgaataat agctcgccgt tctactccac
    14041 catgggcttc ctggtgcgta agggcaaccc gaagaatatc cacgactgga acgacctggt
    14101 gcgctccgac gtgaagctga ttttcccgaa cccgaaaacg tcgggtaacg cgcgttatac
    14161 ctatctggcg gcatggggcg cagcggataa agctgacggt ggcgacaaag ccaaaaccga
    14221 acagtttatg acccagttcc tgaaaaacgt tgaagtgttc gatactggcg gtcgtggcgc
    14281 gaccaccact tttgccgagc gcggcctggg cgatgtactg atcagcttcg agtcggaagt
    14341 gaacaacatc cgtaaacagt atgaagcgca aggctttgaa gtggtgattc cgaaaaccaa
    14401 cattctggcg gaattcccgg tggcgtgggt cgataaaaac gtgcaggcca acggtacgga
    14461 aaaagcagca aaagcctacc tgaactggct ctacagcccg caggcgcaga ccatcatcac
    14521 cgactattac taccgcgtaa ataacccgga agtcatggac aaactgaaag ataaattccc
    14581 gcagaccgag ctgttccgcg tggaagacaa atttggctcc tggccggaag tgatgaaaac
    14641 ccacttcacc agcggcggcg agttagacaa gctgttagcg gcggggcgta actgatgttt
    14701 gcagtttcat caagacgcgt gctgccgggc tttaccttaa gcctcggcac cagtctgctg
    14761 tttgtgtgcc tgattttgct gctgccgctc tccgcgctgg tgatgcagct ggctcagatg
    14821 agctgggcgc agtactggga ggtgatcacc aacccacaag tggtcgcggc ctacaaagtg
    14881 acgctgctgt cggcgtttgt ggcatcgatt tttaacggcg ttttcggtct gctgatggcg
    14941 tggattttaa cacgttatcg ctttccgggc cgcacgctgc ttgatgcgct gatggattta
    15001 ccctttgcgc tgccaacggc agtcgccggt ttaacgctgg cctcgctctt ttccgtaaac
    15061 ggtttttacg gtgaatggct ggcgaagttt gatatcaaag tcacctatac ctggcttggt
    15121 attgcggtgg cgatggcctt taccagcatt ccgtttgtgg tgcgtaccgt gcagccggtg
    15181 ctggaagagt taggcccaga atatgaagaa gcggcggaaa cgcttggtgc aacgcgctgg
    15241 cagagtttct gcaaagtggt gctgccggaa ctttctccag cgctggtggc gggcgtggcg
    15301 ttgtcgttta cccgtagtct tggtgagttt ggcgcggtga tatttatcgc cgggaacatc
    15361 gcttggaaga cggaagtgac gtcgctgatg atttttgtgc gcttgcagga gtttgattac
    15421 ccggcggcga gcgcgattgc ttcggtgatc ctcgcggcat ccctgctgct gctgttctca
    15481 attaacactc tgcaaagtcg ctttggtcgg cgtgtggtag gtcattaatg gcggaagtta
    15541 cccaattgaa gcgttatgac gcgcgcccga ttaactgggg caaatggttt ctgattggca
    15601 tcgggatgct ggtatcggcg ttcatcctgc tggtgccgat gatttatatc ttcgtacagg
    15661 cgttcagcaa agggctgatg ccggttttac agaatctggc cgatccggac atgctgcacg
    15721 ccatttggct gacggtgatg attgcgctga ttgccgtacc ggtgaacctg gtgttcggca
    15781 ttctgctggc gtggctggta acgcgcttta acttccccgg acgccagtta ctgctgacgc
    15841 tactggacat tccgtttgcc gtatcgccgg tagttgccgg tctggtgtat ctgctgttct
    15901 acggctctaa cggtccgctg ggcggctggc tggatgaaca taatttgcag attatgttct
    15961 cctggccggg aatggtgctg gtcaccatct ttgtgacctg tccttttgtg gtgcgcgagc
    16021 tggtgccggt gatgttaagc cagggcagcc aggaagacga agcggcgatt ttgcttggcg
    16081 cgtctggctg gcagatgttc cgtcgcgtca cattgccgaa cattcgctgg gcgctgctgt
    16141 atggtgtggt gctaaccaac gcccgcgcga ttggcgagtt tggtgcggtg tcggtggttt
    16201 ccggctcgat tcgcggcgaa acactttcgc tgccgttaca gattgaattg ctggagcagg
    16261 actacaacac cgtcggctcc tttaccgccg ccgcgctgtt gacgctgatg gcgattatca
    16321 ccctgttttt aaagagtatg ttgcagtggc gtctggagaa tcaggaaaaa cgcgcgcagc
    16381 aggaggagca tcatgagcat tgagattgcc aatattaaga agtcgtttgg tcgcacccag
    16441 gtgctgaacg atatctcact ggatattcct tctggtcaga tggtcgcgct gctggggccg
    16501 tccggttccg gaaaaaccac gctgctgcgc attattgccg ggctggagca tcaaaccagc
    16561 gggcatattc gcttccacgg taccgacgtg agccgcctgc acgcacgcga tcgtaaagtc
    16621 ggtttcgtgt tccagcatta cgcgctgttc cgtcatatga cggtgttcga caatatcgct
    16681 tttggcctga cggtgctgcc acgtcgcgag cgcccgaatg ccgcagccat caaagcgaaa
    16741 gtgacaaaat tgctggagat ggtccagctt gcgcatctgg cggatcgtta tccggcacag
    16801 ctttccggcg gccagaaaca gcgcgtggcg ctggcgcgcg cgctggctgt tgaaccgcaa
    16861 attctcctgc ttgatgaacc gtttggcgcg ctggatgcgc aggtgcgtaa agagctgcgt
    16921 cgctggctgc gtcaactgca tgaagagctg aaattcacca gcgtgttcgt gacccacgac
    16981 caggaagaag cgaccgaagt agctgatcgt gtagtggtga tgagccaggg caatatcgag
    17041 caggctgatg cgccggacca ggtatggcgc gaaccagcga cccgatttgt gctcgaattt
    17101 atgggcgaag tgaaccgcct gcagggaacc attcgcggcg ggcagttcca tgttggcgca
    17161 catcgctggc cgctggggta tacgcctgcg tatcaggggc cggtggatct cttcctgcgc
    17221 ccgtgggaag tggatatcag ccgccgtacc agtctcgatt cgccgctgcc ggtacaggta
    17281 ctggaagcca gcccgaaagg tcactacacc caattagtgg tgcagccgct ggggtggtac
    17341 aacgaaccgc tgacggtcgt gatgcatggc gacgatgccc cgcagcgtgg cgagcgttta
    17401 ttcgttggtc tgcaacatgc gcggctgtat aacggcgacg agcgtatcga aacccgagat
    17461 gaggaacttg ctctcgcaca aagcgcctga taggttgagt gaatgttaaa cgcccggagg
    17521 cgcttcccgc gatccgggct ttttaatggc aaggtttgta acctgtagac ctgataagac
    17581 gcgcaagcgt cgcatcaggc aacaccacgt atggatagag atcgtgagta cattagaaca
    17641 aacaataggc aatacgcctc tgataaagtt gcagcgaatg gggccgaata acggcagtga
    17701 agtctggttg aaactggaag gcaataaccc ggcaggttcg gtgaaagatc gtgcggcact
    17761 ttcgatgatc gtcgaggcgg aaaagcgcgg ggaaattaaa ccgggtgatg tgttaatcga
    17821 agccaccagt ggtaacaccg gcattgcgct ggcaatgatt gccgcgctta aaggctatcg
    17881 catgaaattg ctgatgcccg acaacatgag ccaggaacgc cgtgcggcga tgcgtgctta
    17941 tggtgcggaa ctgattcttg tcaccaaaga gcagggcatg gaaggtgcgc gcgatctggc
    18001 gctggagatg gcgaatcgtg gcgaaggaaa gctgctcgat cagttcaata atcccgataa
    18061 cccttacgcc cattacacca ctaccgggcc ggaaatctgg cagcaaaccg gcgggcgcat
    18121 tacccatttt gtctccagca tgggaacaac cggcactatc accggcgttt cacgctttat
    18181 gcgcgaacaa tccaaaccgg tgaccattgt cggcctgcag ccggaagaag gcagcagtat
    18241 tcccggcatt cgccgctggc ctgcggaata tctgccgggg attttcaacg cttctctggt
    18301 ggatgaggtg ctggatattc atcagcgcga tgcggaaaac accatgcgcg aactggcggt
    18361 gcgggaagga atattctgtg gcgtcagctc aggcggcgcg gttgctggag cactgcgggt
    18421 ggcaaaagct aaccctggtg cggtggtggt agcgatcatc tgcgatcgtg gcgatcgcta
    18481 cctctccacc ggagtgtttg gggaagagca ttttagccag ggggcgggga tttaagatta
    18541 ttgcgcaggc accgttttat ccgtctgcgc aattagcgca tgtaaaaaag gctgtgcgtt
    18601 ctcatcgctc ttaccgggga tcttcacaat atacggcttc ccggtaatcg atgacgacga
    18661 agcaacctta tcaataaact gctcggcagt gtcaatgcgg ttacgggtat tgccgagctt
    18721 cagacgcaga tgagaaaccg cttcatcgca ggtatgttca tcaccgttgc gcacaaatat
    18781 caaatccttt ttctgtgata atccctccag catggcgttg atgcgggctt cttcatgggc
    18841 agttaacttc gcgtaagcgg ggagtgtcat tagtagtgta atgaccagac aaatgatttt
    18901 cttcattgaa ttaccttatc cttgttttgt aatagccgat ttgactgcca gaatcaggta
    18961 aagtttcggt tggaatacga tgaggatcat cggcaaggag aaaaaattaa atgagtagtt
    19021 tgttgttgtt taacgataag agcagggcgc tgcaggcgga tatcgtcgcc gtgcagtcgc
    19081 aggtggttta cggcagcgtg ggcaacagca ttgccgtgcc tgctatcaaa cagaacggtc
    19141 tgaatgtttt tgccgtgccg acggtattgc tgagcaatac gccgcattat gacactttct
    19201 acggtggtgc gattccggat gaatggttta gcggttattt gcgtgcgctt caggagcgtg
    19261 atgcgctgcg ccagctgcgc gctgtaacca cgggctatat gggaacggca tcgcaaatca
    19321 aaatccttgc cgagtggctg actgcgctac gcaaagacca tcctgaccta ttgatcatgg
    19381 tcgatccggt gattggcgat attgatagcg gaatttatgt caaacctgac cttcccgagg
    19441 cgtatcgaca atatttactg ccgctggcgc agggaattac ccccaatatc tttgagctgg
    19501 aaatcctgac cggtaaaaat tgccgcgatc tcgacagtgc cattgctgcc gcaaaaagtc
    19561 tgctttcaga cacattaaaa tgggtggtga ttaccagcgc ctccggtaat gaagaaaatc
    19621 aggagatgca ggtggtggtg gtcagtgccg acagcgtgaa tgtcatttcc cattcacggg
    19681 taaaaaccga cctgaaaggg actggcgacc tgttttgtgc tcagcttatc agtggtttgc
    19741 tgaaagggaa ggcgttaaac gatgcggtgc accgtgcggg gttgcgcgta ctggaagtga
    19801 tgcgctacac ccagcagcat gagagcgatg aattgatttt gccgccgctg gcggaagcat
    19861 aaaaaatggc gccgatgg
//
LOCUS       DAZPEB010000059        19774 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZPEB010000059 DAZPEB010000000
VERSION     DAZPEB010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19774
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.060"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..993
                     /gene="wecH"
                     /locus_tag="ACHURJ_001865"
     CDS             <1..993
                     /gene="wecH"
                     /locus_tag="ACHURJ_001865"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFR1280835.1"
                     /translation="QPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIANV
                     LNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINME
                     LALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMAVIGIIANPNTVP
                     QKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFIIS
                     RGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLGI
                     YGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(1035..1472)
                     /gene="yiaA"
                     /locus_tag="ACHURJ_001866"
     CDS             complement(1035..1472)
                     /gene="yiaA"
                     /locus_tag="ACHURJ_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFR1280836.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(1518..1859)
                     /gene="yiaB"
                     /locus_tag="ACHURJ_001867"
     CDS             complement(1518..1859)
                     /gene="yiaB"
                     /locus_tag="ACHURJ_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFR1280837.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(2024..3467)
                     /gene="xylB"
                     /locus_tag="ACHURJ_001868"
                     /pseudo
     CDS             complement(2024..3467)
                     /gene="xylB"
                     /locus_tag="ACHURJ_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
     gene            complement(3584..4861)
                     /gene="xylA"
                     /locus_tag="ACHURJ_001869"
     CDS             complement(3584..4861)
                     /gene="xylA"
                     /locus_tag="ACHURJ_001869"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFR1280838.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVSFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAAKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGR"
     gene            5227..6198
                     /gene="xylF"
                     /locus_tag="ACHURJ_001870"
     CDS             5227..6198
                     /gene="xylF"
                     /locus_tag="ACHURJ_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR1280839.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNGETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALTANNNKIDAVVASNDATAGG
                     AIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAEIAVELGN
                     GQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            6276..7817
                     /gene="xylG"
                     /locus_tag="ACHURJ_001871"
     CDS             6276..7817
                     /gene="xylG"
                     /locus_tag="ACHURJ_001871"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFR1280840.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            7795..8976
                     /gene="xylH"
                     /locus_tag="ACHURJ_001872"
     CDS             7795..8976
                     /gene="xylH"
                     /locus_tag="ACHURJ_001872"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFR1280841.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            9054..10232
                     /gene="xylR"
                     /locus_tag="ACHURJ_001873"
     CDS             9054..10232
                     /gene="xylR"
                     /locus_tag="ACHURJ_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
                     /protein_id="HFR1280842.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNREVML
                     "
     gene            complement(10340..11164)
                     /locus_tag="ACHURJ_001874"
     CDS             complement(10340..11164)
                     /locus_tag="ACHURJ_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFR1280843.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            11484..13514
                     /gene="malS"
                     /locus_tag="ACHURJ_001875"
     CDS             11484..13514
                     /gene="malS"
                     /locus_tag="ACHURJ_001875"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFR1280844.1"
                     /translation="MKLASCFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLINKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASSALREWKKTNPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            13692..14945
                     /gene="avtA"
                     /locus_tag="ACHURJ_001876"
     CDS             13692..14945
                     /gene="avtA"
                     /locus_tag="ACHURJ_001876"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFR1280845.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNVFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(15096..15569)
                     /locus_tag="ACHURJ_001877"
     CDS             complement(15096..15569)
                     /locus_tag="ACHURJ_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFR1280846.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(15671..16519)
                     /gene="yiaJ"
                     /locus_tag="ACHURJ_001878"
     CDS             complement(15671..16519)
                     /gene="yiaJ"
                     /locus_tag="ACHURJ_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HFR1280847.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            16720..17718
                     /gene="yiaK"
                     /locus_tag="ACHURJ_001879"
     CDS             16720..17718
                     /gene="yiaK"
                     /locus_tag="ACHURJ_001879"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HFR1280848.1"
                     /translation="MKVTFEQLKAAFNRALISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            17730..18198
                     /locus_tag="ACHURJ_001880"
                     /pseudo
     CDS             17730..18198
                     /locus_tag="ACHURJ_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
     gene            18316..18789
                     /gene="yiaM"
                     /locus_tag="ACHURJ_001881"
     CDS             18316..18789
                     /gene="yiaM"
                     /locus_tag="ACHURJ_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HFR1280849.1"
                     /translation="MKKILEVILAINLAVLSCIVFINIILRYGFQTSILSVDELSRYL
                     FVWLTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLK
                     TIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQGA"
     gene            18792..>19774
                     /locus_tag="ACHURJ_001882"
     CDS             18792..>19774
                     /locus_tag="ACHURJ_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HFR1280850.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPDMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSTWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVG"
BASE COUNT         4989 a         4952 c         4804 g         5029 t
ORIGIN      
        1 cagcccaaaa tttactggat tgataacctg cgagggatag cgtgtttaat ggtggtgatg
       61 attcacacca ctacctggta tgtgaccaat gctcatagtg ttagccccgt cacatgggat
      121 atcgccaatg ttctgaattc tgcctctcgt gtcagcgtgc cgctattttt catgatttcc
      181 ggctatctct tttttggcga acgcagcgcc cagccgcgcc atttcttgcg tatcggctta
      241 tgtctgattt tttatagcgc aatcgcactg ctctacattg cgctgtttac ctccatcaat
      301 atggagttag cgctgaaaaa cctgctgcaa aagccagtgt tttaccactt gtggtttttc
      361 ttcgcgattg cggtgattta tctggtttca ccgctgattc aggtgaagaa cgtcggcgga
      421 aaaatgttgc tggtgctaat ggcggtgatt ggcattatcg ctaacccaaa cacagtgccg
      481 cagaaaattg acggttttga atggctgcca attaacttat atatcaatgg cgatactttt
      541 tactacattc tgtatggcat gttgggccgc gctataggga tgatggacac acagcataaa
      601 gcactgtcgt gggtgagcgc cgcgctgttt gcgacggggg tttttattat ctctcgcggg
      661 acattatatg aattgcagtg gcgcggaaat tttgccgata cctggtatct ttactgtggg
      721 ccgatggttt ttatctgcgc aatcgcgcta ttgactctgg ttaaaaacac gctggatacg
      781 cgtaccattc gcggacttgg cttaatctcc cgccattcat tgggtatata cggattccac
      841 gccttgatta tccatgcgct gcgcacccgg ggaattgagc ttaaaaattg gccaatactg
      901 gatattattt ggatcttttg cgcgacgttg gcagcgagtt tgttactttc tatgctggta
      961 caacgaatcg acagaaacag attagtgagt taagtaaaag cccgttcaca ttggactgac
     1021 cgggcttacg tgaattattc gctgtattct tcctgggtaa cctgtgtttc ttttggcggg
     1081 tttattccgg catcacgaat attcttctgc accgctactg caccaaacaa gcttaagaag
     1141 aaagccagtc cataaaaacc tttttcactg agtaataatg tcgcattcca cagacctacc
     1201 atcagtaatg caacagagat aataaagaca gtcaggcagg tcatataata aatggaagtg
     1261 gtcggtatgc cttcatactt gtcccgaacg gtcttttgat aagacgcagc agaaaacagt
     1321 cctaacacca gtacggcaaa ataataccct ttttcattta actgcatctc tgcattccat
     1381 agccctaaca gataggtaac gataccacca acgagagcta tccatgacac aatactaaag
     1441 gccggtgaat aggttgatat tttgttgtcc atcacagtat tcctttcatt cctgaatgtg
     1501 taagagcttt atgttgctca taccgagcga aacaaacgca tttgaccaaa caagcagaca
     1561 aaaaaggcga tggggtagat caccatttca taagcattga taggtgcatt taaaacaccg
     1621 ataaacaaga gaccgatagt gattaacgca actaactggc agatatgggt aaaaaaatta
     1681 tccagttgcc cgagtttttc tgcgcgaaga tatgcatagt taccaaaagt tgctgtcatt
     1741 aacacgccaa gaaaatagcc ttttccactt agcaatgggc atgagatcca tagcccaacc
     1801 agataaacca gcgccccgac aacaaataat agttttgcca cagtttttga ggttttcata
     1861 acgatctcca tatctaccag cgatacatta cgagtaacca acgaaagaca aaactgaaaa
     1921 atgccattaa caaatgattt tcagaataaa ttcatactaa atattaatta gtgagatata
     1981 tagatgtgaa ttatccccca cccggtcagg caggggataa cgtttacgcc attaatggca
     2041 gaagttgctg atagaggcga cggaacgttt cacgtcgtgg ctgataagcg gcataacgct
     2101 gcgcatctgg tagatgcgac tgttctaacg gtagctgcgg caacaactca atgagcgatt
     2161 tctctggatt cgccgcaatc tgcgccagcc ttgctgcgcc cagtgctggc cccacatccc
     2221 cccgtacggt aatcgagctg ctgaccgctg atatccgcca gcatctgacg ccagtactca
     2281 ctacgcgccc cgccaccaat caacgtaaca ctttgcggtt taatgccgca ggcatgcacg
     2341 acatccatgc catctgccag cgcataaccc acgccttcca gcactgctcg cgccagttca
     2401 ttggggccat gttgatgagt caaaccaaag aaaaccccct tcgcctgggg attattgtgt
     2461 ggtgtacgct cgccggaaag ataaggcaga aaccaaactg gctcggcact ttcatcagcc
     2521 tgttgagctg cagcgattaa agctgggaca ttgcacaggc cggttaattt cgcgacccaa
     2581 tccagacacg atgctgcact cagcattaca gacattaaat gccaacgctg cggtagcgca
     2641 tggcaaaagc tgtgtacagc gctttctggc ttgcttaaga acccttcgct gacagcaaaa
     2701 tagacccccg acgtccccag cgataacatt gcctgattag catcaaccat tcccacacca
     2761 actgcattgt cgccaccgcc tgcgacaact ggcaccgtcg ccatacccca cgctttcgca
     2821 acttcaggta acaaagcacc agtaatttcg ctgccttcgt ataatgcggg catctggtca
     2881 cgagataagt cgcaagcctg cagcatgacg tcactccagt cacgctttgc gacatccagc
     2941 cacatggtgc cagctgcgtc agacatatcg ctggcaaact cccccgtcat acgcagacgc
     3001 aagtaatctt tcggtaataa tactttgtcg atttgacgga atatctccgg ctcatgccgc
     3061 tgaacccata gcaatttagg cgcagtaaat ccgggcatca tcaggttgcc ggtaatcact
     3121 cgtgattgcg gaactctcgc ttccagcaaa gtgcactctt gcgcacagcg cccgtcgttc
     3181 cacaaaatgg cagggcgcaa tacccgttgt tgagcatcca gtaaggttgc tccatgcatc
     3241 tggccggcaa tacccaatgc tttaacgtcc tgcagagaat gctgatcgcc cagagctttc
     3301 attgcgcgat cagttgcctg ccaccactgt tccgggtctt gttccgacca gagtggatgc
     3361 gggcgcgaaa cggtcagctt ttccgtttgc gaagcaacca cctcaccctg ctcgttgagc
     3421 aaaataactt ttacgcccga ggtgccaaga tctatcccga tatacatatc gatcgttcct
     3481 taaaaaaatg cccggtatcg ctaccgataa ccgggccaac ggactgcaca gttagccgtt
     3541 atttgtcgaa cagataatga tttaccagat tttccagttg ctcctagcga ccactctgat
     3601 gcaccggaga caaattatgt tcctgagcat atttggctaa atctgccagt gacatttggc
     3661 ctttcaggat ttgctggccc aattcgctat tccagccgga ataacgctgc gcgatgcgtt
     3721 tatccagctc gccatcttca atcatgcgcg ctgcaatttt cagcgccagt gccatcgtat
     3781 ccatcgcgcc gatatgaccg taaaacagat catatttatc agtactttga cgacgtactt
     3841 tggcatcgaa gttcagacca ccggtggtga aaccgcctgc tttgagaatt tcatacatca
     3901 ccagcgcatt ctcttccaca ctgttcggga actggtcggt gtcccagccc agttgcgcat
     3961 cgccacggtt ggcgtcgaca gaaccgaaca ggccaagcgc aatggcggtg gctatttcat
     4021 gatggaaaga gtgacctgcc agcgtcgcgt ggttagcttc aatgttcagt ttaatctctt
     4081 tttccagacc aaactgtttc aggaagccat agaccgtcgc ggcatcgtaa tcatattgat
     4141 gtttagtcgg ttcttgcggt ttcggttcga taagcaacgt gccctggaag ccgattttat
     4201 gtttatgctc aaccaccatc tgcataaagc ggcccagttg ttcacgctcc tgacgcaagt
     4261 cggtatttaa cagcgtttcg taaccttcac gaccacccca caggacatag ttttcaccgc
     4321 ccaatttatg ggttgcttcc atcgctgtaa caacttgcgt tgccgcccag ctgaagactt
     4381 caggatctgg gttcgtcgcc gcacccgcgc catagcgagg gtttgtaaag cagttagcgg
     4441 ttccccacag cagcttcacg ccgctctctt cttgcttggc tgccaggaca tcaaccattt
     4501 gcgcaaaatt attgatgtac tcttttaacg acgcgccctc aggggaaaca tccacatcgt
     4561 ggaagcaata aaatgacaca tgtaacttgt ggaaaaactc aaatgcgaca tctgctttac
     4621 gcttcgccaa cgccagtgcc tcaccaggct gctgccacgg acgattaaac gcccccacac
     4681 caaacatgtc cgccccgttc cagcagaagg tgtgccagta acaggcggca aaacgcaagt
     4741 gctcttccat acgcttaccc aacaccagtt cgtcgggatt gtagtgacgg aatgctaacg
     4801 ggtttgagga ttttgagcct tcataacgaa cgcgatcgag ctggtcaaaa taggcttgca
     4861 tattgaactc cataatcagg taatgccgcg ggtgatggat gatgtcgtaa tattgggcac
     4921 tccctttcag ttgctcaatt atgttatttc acactgctat tgagataatt cacaagtgtg
     4981 cgctcgctcg caaaataaaa tgggatgatg aaactgggta attcctcgaa gataaaaatg
     5041 caataagtac aattgcgcaa caaaagtaag atctcggtca taaatcaaga aataaaccaa
     5101 aaatcgtaat cgaaagataa aaatctgtaa ttgttttccc ctgtttagtt gctaaaaatt
     5161 ggttacgttt atcgcggtga ttgttactta ttaaagctgt cctctaacta cagaaggccc
     5221 tacaccatga aaataaagaa cattctactc accctttgca cctcacttct gcttaccaac
     5281 gttgcggcac acgccaaaga agtcaaaata ggtatggcga ttgatgatct acgtcttgaa
     5341 cgctggcaaa aagatcgaga tatttttgtg aaaaaggctg aatctctcgg tgcgaaagta
     5401 tttgtacagt ctgcaaatgg caatggagaa acacaaatgt cgcagattga aaacatgatt
     5461 aaccggggtg tcgatgttct tgtcattatt ccgtataacg gccaggtatt aagtaacgtt
     5521 gtaaaagaag ccaaacaaga aggtattaaa gtattagctt acgaccgtat gattaacgat
     5581 gcggatatcg atttttatat ttctttcgat aacgaaaaag tcggtgaact gcaggcaaaa
     5641 gccctggtcg atattgttcc gcaaggtaat tacttcctga tgggcggctc gccggtagat
     5701 aacaacgcca agctgttccg cgccggacaa atgaaagtgt taaaacctta cgttgattcc
     5761 ggaaaaatta aagtcgttgg tgaccaatgg gttgatggct ggttaccgga aaacgcgcta
     5821 accgccaata ataacaaaat tgatgctgta gttgcctcaa acgatgccac cgcaggtggg
     5881 gcaattcagg cattaagcgc gcaaggttta tcagggaaag tagcaatttc cggccaggat
     5941 gcggatctcg caggtattaa acgtattgct gccggtacgc aaactatgac ggtgtataaa
     6001 cctattacat tgctggcaaa tactgccgca gaaattgccg ttgaattggg caatggtcag
     6061 gagccaaaag cagatacctc actgaataat ggcctgaaag atgtcccctc ccgcctcctg
     6121 acaccgatcg atgtgaataa aaacaacatc aaagatacgg taattaaaga cggattccac
     6181 aaagagagcg agctgtaagc gttacgcccc ggcgcggagc gggggcgtga tttctctcca
     6241 tgccgcgtga atgaattggc ttaggtggag tcgttatgcc ttatctactt gaaatgaaga
     6301 acattaccaa aaccttcggc agtgtgaagg cgattgataa cgtcagcttg cggttgaatg
     6361 ctggcgaaat cgtctcactc tgtggggaaa atgggtctgg taaatcaacg ctgatgaaag
     6421 tgctgtgtgg tatttatccc catggctcct acgaaggcga aattattttt gcgggagaag
     6481 agattcaggc gagtcacatc cgcgataccg aacgcaaagg tatcgccatc attcaccagg
     6541 aattggccct ggtgaaagaa ttgaccgtgc tggaaaatat cttcctgggt aacgaaataa
     6601 cccacaatgg cattatggat tatgacctga tgacgctacg ctgtcagaag ctgctcgcac
     6661 aggtcagttt atccatttca cctgataccc gcgttggcga tttagggctt gggcaacaac
     6721 aactggttga aattgccaag gcacttaata aacaggtgcg cttgttaatt ctcgatgaac
     6781 cgacagcctc attaactgag caggaaacgt cggttttact ggatattatt cgcgatctac
     6841 aacagcacgg tatcgcctgt atttatattt cgcacaaact caacgaagtc aaagcgattt
     6901 ccgatacgat ttgcgttatt cgcgacggtc agcacattgg tacgcgtgat gctgccggaa
     6961 tgagtgaaga cgatattatc accatgatgg tcgggcgaga gttaaccgcg ctttacccta
     7021 atgaaccaca taccaccgga gatgaaatat tacgtattga acatctgacg gcatggcatc
     7081 cggttaatcg tcatattaaa cgagttaatg atgtctcgtt ttccctgaaa cgtggcgaaa
     7141 tactgggtat tgccggactc gttggtgccg gacgtaccga gaccattcag tgcctgtttg
     7201 gcgtgtggcc cggacaatgg gaaggaaaaa tttatattga tggcaaacag gtagatattc
     7261 gtaactgtca gcaagccatc gcccagggga ttgcgatggt acccgaagac agaaagcgcg
     7321 acggcatcgt tccggtaatg gcggttggta aaaatattac cctcgccgca ctcaataaat
     7381 ttaccggtgg cattagccag cttgatgacg cggcagagca aaaatgtatt ctggaatcaa
     7441 tccagcaact caaagttaaa acgtcgtccc ccgatcttgc tattggacgt ttgagcggcg
     7501 gcaatcagca aaaagcgatc ctcgcccgct gtctgttact caacccgcgc attctcattc
     7561 ttgatgaacc taccaggggt attgatattg gcgcgaaata tgagatctac aaattaatta
     7621 accaactcgt ccagcagggt attgccgtta ttgtcatctc ttccgaatta cctgaagtgc
     7681 tcggccttag cgatcgtgta ctggtgatgc atgaagggaa actaaaagcc aacctgataa
     7741 atcataacct gactcaggag caggtgatgg aagccgcatt gaggagcgaa catcatgtcg
     7801 aaaagcaatc cgtctgaagt gaaattggcc gtaccgacat ccggtggctt ttccgggctg
     7861 aaatcactga atttgcaggt cttcgtgatg attgcagcta tcatcgcaat catgctgttc
     7921 tttacctgga ccaccgatgg tgcctactta agcgcccgta acgtctccaa cctgttacgc
     7981 cagaccgcga ttaccggcat cctcgcggta ggaatggtgt tcgtcataat ttctgctgaa
     8041 atcgaccttt ccgtcggctc aatgatgggg ctattaggtg gcgtcgcggc gatttgtgac
     8101 gtctggttag gctggccttt gccacttacc atcattgtga cgttggttct gggactgctt
     8161 ctcggtgcgt ggaacggatg gtgggtcgcg taccgtaaag tcccttcatt tattgtcacc
     8221 ctcgcgggca tgttggcatt tcgcggcata ctcattggca tcaccaacgg cacgactgta
     8281 tcccccacca gcgccgcgat gtcacaaatt gggcaaagct atctccccgc cagtaccggc
     8341 ttcatcattg gcgcgcttgg cttaatggct tttgttggtt ggcaatggcg cggaagaatg
     8401 cgccgtcagg ctttgggttt acagtctccg gcctctaccg cagtagtcgg tcgccaggct
     8461 ttaaccgcta tcatcgtatt aggcgcaatc tggctgttga atgattaccg tggcgttccc
     8521 actcctgttc tgctgctgac gttgctgtta ctcggcggaa tgtttatggc aacgcggacg
     8581 gcatttggac gacgcattta tgccatcggc ggcaatctgg aagcagcacg tctctccggg
     8641 attaacgttg aacgcaccaa acttgccgtg ttcgcgatta acggattaat ggtagccatc
     8701 gccggattaa tccttagttc tcgacttggc gctggttcac cttctgcggg aaatatcgcc
     8761 gaactggacg ctattgcagc atgcgtgatt ggcggcacca gcctggctgg cggtgtggga
     8821 agcgttgccg gagcagtaat gggggcattt atcatggctt cactggataa cggcatgagt
     8881 atgatggatg taccgacctt ctggcagtat atcgttaaag gtgcgattct gttgctggca
     8941 gtatggatgg actccgcaac caaacgccgt tcttgatttt gataaaaatt ttctcaaagc
     9001 cggttacgta ttaccggttt tgagtttttg catgattcag caggaaaaga accatgttta
     9061 ctaaacgtca ccgcatcaca ttactgttca atgccaataa agcctatgac cggcaggtag
     9121 tagaaggcgt aggggaatat ttacaggcgt cacaatcaga atgggatatt ttcattgaag
     9181 aagatttccg cgcccgcatt gataaaatca aggactggtt aggagatggc gtcattgccg
     9241 acttcgacga caaacaaatc gagcaagcgc tggctgatgt cgacgtcccc attgttgggg
     9301 ttggcggctc gtatcacctt gcagaaagtt acccacccgt tcattacatt gccaccgata
     9361 actatgcgct ggttgaaagc gcatttttgc atttaaaaga gaaaggcgtt aaccgctttg
     9421 ctttttatgg tcttccggaa tcaagcggca aacgttgggc cactgaacgc gaatatgcat
     9481 ttcgtcagct tgtcgccgaa gaaaagtatc gcggagtggt ttatcagggg ttagaaaccg
     9541 cgccagagaa ctggcaacac gcgcaaaatc ggctggcaga ctggctacaa acgctgccac
     9601 cgcaaaccgg gattattgcc gttactgacg cccgggcgcg gcatattctg caagtatgtg
     9661 aacatctaca cattcccgta ccggaaaaat tatgcgtgat tggcatcgac aacgaagaac
     9721 tgacccgcta tctgtcgcgt gtcgcccttt cttcggtcgc tcagggcgcg cggcaaatgg
     9781 gctatcaggc ggcaaaactg ttgcatcgat tattagataa agaagaaatg ccgctacagc
     9841 gaattttggt cccaccagtt cgcgtcattg aacggcgctc aacagattat cgctcgctga
     9901 ccgatcccgc cgttattcag gccatgcatt acattcgtaa tcacgcctgt aaagggatta
     9961 aagtggatca ggtactggat gcggtcggga tctcgcgctc caatcttgag aagcgtttta
    10021 aagaagaggt gggtgaaacc atccatgcca tgattcatgc cgagaagctg gagaaagcgc
    10081 gcagtctgct gatttcaacc accttgtcga tcaatgagat atcgcaaatg tgcggttatc
    10141 catcgctgca atatttctac tctgttttta aaaaagcata tgacacaacg ccaaaagagt
    10201 atcgcgatgt aaatcgcgag gtcatgttgt agcgatgatg agaattgtcg gcgtcacatc
    10261 agggaactca gcacagccac ctgatgcgag caaaagcctt atccgaccga caaatcagat
    10321 aaggctcaag gaaatgcaat tacatatgcg ccgcgattaa ccgttggtta tcctggtaca
    10381 ttgcgaacag gtagttatta taactcttcc ctttggtcga gtagcccttc agcttgtgaa
    10441 tcatcgctgt ggcagtcact tcctgatccg ctttacgcag ctgcgcacgc gatttacgga
    10501 acgaagagta agccgggtgc gtattcaggt tagtgacata ggcgctcacc gattctttga
    10561 cagaactaaa ctgtgagtac cctttcactt tacccggcgc attggtacaa cgtcctttca
    10621 tgcatttcat gccgaacaga ttgttattgt tgcgcgccag cttcgacgtt ccccaaccgc
    10681 tttctgctgc agccatcgtc gccaccatac tggtagggat aatgtctacg cgttcaagca
    10741 aggtattcca cgggattttt cgcgtattac cagaccactt caccttgtag cgtttggcga
    10801 tgtctttcag acgcgcacgc tcagcaggtg accattggcc ctgatactgt tttgaaatca
    10861 gccagttacg ttctgcagta atggctgcat tttggctggt aatgtaaggc attacggtcc
    10921 ggagaaacgc ctttttcctt ggtgttccgg aagggtattt tcgcaaatca ggaagtgaac
    10981 tactctttgc actattgcga gaatactctt gtttactgct tacctgttta ttactagctt
    11041 tagttaagtg ggacttttga ctcgctgttg ttgtgtgcgt cttcgctaac acctcactcg
    11101 aaaacaccag agtgagtaac ataagaatca tcgccccata tcgtcgtatg ggagtcaaaa
    11161 tcatcaggtc tcctggtcgg atttaatcat tccaacacct tatatttttc acaaatttga
    11221 gagttgaatc tcaaatcata tcaaaaatag ctgtcaagag caccccaagg aatagtccaa
    11281 atctgaaact atgtcacgag ttaacgattc agattggcgc taaatcgcag aaaatgtggg
    11341 gtttatcgca aaattcagcc gttttttgcg cgcgatcgct cacccttgct tctcatcctg
    11401 tggacttacc gctcagggat gagttttgtc tggcttatcg ctggcaaact gtctgaaatc
    11461 gcagcaataa ggactcatcc accatgaaac tcgcctcctg ttttctgaca ctccttcctg
    11521 gcttcgccgt tgccgccagc tggacttctc cggggttccc tgcctttagc gaacagggaa
    11581 cgggaacatt tgtcagccac gcgcagttgc ccaaaggtac gcgtccactc acgctaaatt
    11641 ttgaccaaca gtgctggcag cctgcagatg cgataaaact caatcagatg ctttccctgc
    11701 aaccttgtag caacacgccg cctcaatggc gattgttcag ggacggcaaa tatacgctgc
    11761 aaatagacac ccgctccggt acgccaacat tgatgatttc catccagaac gccgccgaac
    11821 cggtagcaaa cctggtccgt gaatgcccga aatgggatgg attaccgctc acgctggatg
    11881 tcagcgccac tttcccggaa ggagccgccg tacgggatta ttacagccag caaattgcga
    11941 tagtgaagaa cggtcaaata acgttacaac ccgctgctac cagcaacggt ttactcctgc
    12001 tggaacgggc agaaactgac acatccgccc ctttcgactg gcataacgcc acggtttact
    12061 ttgtgctgac agatcgtttc gaaaacggcg atcccagtaa tgaccagagt tacggacgtc
    12121 ataaagacgg tatggcggaa attggcactt ttcacggcgg cgatttacgc ggcctgatca
    12181 acaaactgga ttacctccag cagttgggcg ttaatgcttt atggataagc gccccatttg
    12241 agcaaattca cggctgggtc ggcggcggta caaaaggcga tttcccgcat tatgcctacc
    12301 acggttatta cacacaggac tggacgaatc ttgatgccaa tatgggcaac gaagccgatc
    12361 tacggacgct ggttgatagc gcacatcagc gcggtattcg tattctcttt gatgtcgtga
    12421 tgaaccacac cggctatgcc acgctggcgg atatgcagga gtatcagttt ggcgcgttat
    12481 atctttctgg tgacgaagtg aaaaaaacgc tgggtgaacg ctggagcgac tggaaacctg
    12541 ccgccgggca aacctggcat agctttaacg attacattaa tttcagcgac aaaacaggct
    12601 gggataaatg gtggggaaaa aactggatca gaacggatat cggcgattac gacaatcctg
    12661 gattcgacga tctcactatg tcgctggcct ttttgccgga tatcaaaacc gaatcaacta
    12721 ccgcttctgg tctgccggtg ttctataaaa acaaaacgga tactcacgct aaagtcatcg
    12781 aaggctttac acctcgcgat tacttaaccc actggttaag tcagtgggtc cgcgactatg
    12841 ggattgatgg ttttcgggtc gataccgcca aacatgttga gttgcccgcc tggcagcaac
    12901 tgaaaaccga agccagctcc gcgcttcgcg aatggaaaaa aactaacccc gacaaagcat
    12961 tagatgacaa acctttctgg atgaccggtg aagcctgggg ccacggcgtg atgcaaagtg
    13021 actactatcg ccacggcttc gatgcgatga tcaatttcga ttatcaggag caggcggcga
    13081 aagcagtcga ttgtctggcg cagatggata cgacctggca gcaaatggcg gagaaattgc
    13141 agggtttcaa cgtgttgagc tacctctcgt cgcatgatac ccgtctgttc cgtgaagggg
    13201 gcgacaaagc agcagagtta ttactattag cgccaggcgc ggtacaaatc ttttatggcg
    13261 atgaatcctc gcgtccgttc ggtcctacag gttctgatcc gctgcaaggt acacgttcgg
    13321 atatgaactg gcaggatgtt agcggtaaat ctgccgccaa cgtcgcgcac tggcagaaaa
    13381 tcagccagtt ccgcgcccgc catcccgcaa ttggcgcggg caaacaaacg acactttcgc
    13441 tgaagcaggg ctacggcttt gttcgtgagc atggcgacga taaagtgctg gtcatctggg
    13501 ctgggcaaca gtgacttttc cggcttcccg ttcatcagta cctcgggaag ccgccaacca
    13561 ggataaaatg tcagccctaa tcagcgttgc aggataaagc accgctcact cttcaacaga
    13621 ccgatttgca ccccagcaaa tgtagcgtta ttgttacctt ccttgctaca gagttcgaca
    13681 gatatcccgc tatgacattc tccctttttg gtgacaaatt tacccgccac tccggcatta
    13741 cgctgctgat ggaagatctg aacgacggtt tacgcacgcc tggcgcgatt atgctcggcg
    13801 gcggtaatcc ggcgcagatc ccggaaatgc aggactactt ccagacgcta ctgaccgaca
    13861 tgctggaaag tggcaaagcg actgatgcac tgtgtaacta cgacggtcca caggggaaaa
    13921 cggagctact cacactgctt gccggaatgc tgcgcgagaa gttgggttgg gatatcgaac
    13981 cacagaatat tgcactaaca aacggcagcc agagcgcgtt tttctactta tttaacctgt
    14041 ttgccggacg ccgtgccgat ggtcgggtca aaaaagtgct gttcccgctg gcaccggaat
    14101 acattggcta cgctgacgcc ggactggaag aagatctgtt tgtctctgcg cgtccgaata
    14161 ttgaactgct gccggaaggc cagtttaaat accacgtcga ttttgagcat ctgcatattg
    14221 gcgaagaaac cgggatgatt tgcgtctccc ggccgacgaa tccaacaggc aatgtgatta
    14281 ctgacgaaga gttgctgaag cttgacgcgc tggcgaatca acacggcatt ccgctggtga
    14341 ttgataacgc ttatggcgtc ccgttcccgg gtatcatctt cagtgaagcg cgcccgctat
    14401 ggaatccgaa tatcgtgctg tgcatgagtc tttccaagct ggggctgcct ggctcccgct
    14461 gcggcattat catcgccaat gaaaaaatca tcactgccat caccaatatg aacggcatta
    14521 tcagcctggc acctggcggt attggtccgg cgatgatgtg tgaaatgatt aagcgtaacg
    14581 atctgctgcg cctgtctgaa acagtcatca aaccgtttta ctaccagcgt gttcaggaaa
    14641 ctatcgccat cattcgccgc tatttaccgg aaaatcgctg cctgattcat aaaccggaag
    14701 gagccatttt cctctggcta tggtttaagg atttgcccat tacgaccgag cagctctatc
    14761 agcgcctgaa agcacgcggc gtgctgatgg tgccggggca caacgtcttc ccgggactgg
    14821 ataaaccgtg gccgcatacg catcaatgta tgcgcatgaa ctacgtacca gagccggaga
    14881 aaattgaggc gggggtgaag attctggcgg aagagataga aagagcctgg gctgaaagtc
    14941 actaaaaatt gcctgatgcg ctacgcttat caggcctaca tgatttctgc aatatattga
    15001 atttggaaga atttgtaggc cggataaggc gtttacgccg catctggcat aaacaaagcg
    15061 cactattccg gcgttgagaa acgccggaaa acgtttcaaa caggctgctg ccgtagccga
    15121 tgccgctgca ctttctcgac atccatgcac tgcaacgcct ttgtcgggca ggcttcaaca
    15181 caggccggcc ccgtctcccg atgccagcac agatcgcact taatcgccct cgcctttttg
    15241 cgcgaagaaa cgacctccat cgcaccaaac gggcaagcca gcatacagct tttacagcca
    15301 atgcaacgtg tttgttcaac gaaaatatgc ccatgttcgc ggcttatcgc gtcaacaggg
    15361 cagacattcg cgcacggtgc atcttcacac tgatgacagg ctactgccgt ggtccagctg
    15421 tggtctttaa tgacacgaat acgggaaata aactcgtctg gtgacaacgc agcgcaatcc
    15481 tgattctcct gatgcgacac tgcgcaagcc acttcacagg tacggcaacc gatacatttc
    15541 gtcgcatccg caataataaa ccggttcatg atcactcctt tgcactaaaa cagcagagtg
    15601 ccagagagcg cgtccctact gctttgatcc aacgaaggaa atgttaaagt tttggcactt
    15661 gtccaaagcg ttatgtgatc gcgcccaggt catccctgac agtaaatccc agttcattag
    15721 aaatagcctg cgcggtttca cgcagtggtt tcaggagatt tttctctccc acctgtttca
    15781 gacgtgatgt cgaaagcgaa atcgacacgg cgtacggcac ccgcccatga atatcaaaca
    15841 ccggaacagc aatacaggag acgccgagtt cgttttcttc tctgtccatc gccgctccgc
    15901 tttcacgaat gtgcgccagt tcgtcgaaca tcgcgggcag ctcggtaatg gtattgcggg
    15961 ttaacggctg gatctcatgc tgatggtttt cccagtatga cttcacgtag tccgggtgac
    16021 caaacgccat gtagatcttg cccattgcgg aacagtagag cggcatatgc tggccaatat
    16081 aggcacgggt tcgcagcatc ccggttgtgg gttccagctt ataaatcaaa atagcgtgat
    16141 cgtcttcgcg gctggagaag ttaatggttt caccagtggc gatgttcagt gcctcaagat
    16201 gcggagcggc gatatgaatg atattcagcg aagacagcgc cttctgcccg acggcaataa
    16261 atttggtagt caggcggtaa ctccctgcgg cgggcgcggt ggtcacatag ccgcaggact
    16321 gtaatccctg caataagcga tggacggtac tcttatttaa accagccagc tccgaaagat
    16381 gcgccaacgg acaaccgttt ggatagttgc tcaaaatctc aatcagcatc aacccgcgaa
    16441 acagactctg gcttccggct ggacgctctt tttcctgcgc catctcgttc tctttttttc
    16501 ccatcacttc tttccccatt ttgtcgcgtc ctgatggtag cgcaaagtgt gccgtagttc
    16561 acgatctcga cagataattt ataaccactt gatttttatg tcttttgaaa ttcatcaatc
    16621 agattgcttt gttaaaaagt gatcgatata tttgaaatca agtttcgcat attgaaattt
    16681 taagccaaaa aagcgatcaa aaaaacaagg aagcctggga tgaaagtgac atttgagcag
    16741 ttaaaagcag cctttaatcg ggccttaatt tcacgcggcg ttgacagcga aacggctgac
    16801 gcctgtgcag agatgttcgc ccgcaccacc gaatccggcg tttattctca cggcgttaat
    16861 cgtttccctc gtttcattca acaactggaa aacggcgata tcattcctga tgcccaaccc
    16921 aaacgtataa ccagcctcgg cgcaattgaa cagtgggatg cccagcgttc gatcggcaac
    16981 ctgacggcga aaaagatgat ggatcgcgcc attgaactgg ctgccgatca cggtattggt
    17041 ctggtggcac ttcgtaatgc caaccactgg atgcgcggcg gcagctacgg ctggcaggcg
    17101 gcggaaaaag gctatattgg catttgctgg accaactcca tcgccgtaat gccgccgtgg
    17161 ggcgcaaaag agtgtcgcat tggcactaac ccgctgatcg tcgccattcc ttccacgccg
    17221 atcaccatgg tcgatatgtc gatgtcgatg ttctcttacg gcatgttaga agttaaccgc
    17281 ctggcgggcc gtcagctccc ggtcgatggt ggctttgatg atgagggcaa tttgaccaaa
    17341 gaacctggcg ttatcgagaa gaatcgccgc attttgccga tgggctactg gaaaggttct
    17401 ggcatgtcga ttgtgctgga tatgatcgct actctccttt ccgacggcgc atcggttgcc
    17461 gaagtcaccc aggacaacag cgacgaatac ggcatttcac aaatttttat tgccattgaa
    17521 gtggacaagc ttatcgacgg tcccacccgc gatgccaagc tgcaacgcat catggattac
    17581 gttactaccg ctgaacgcgc tgacgaaaac caggccatcc gcttacccgg ccacgaattt
    17641 actaccctgc tggccgaaaa ccgccgtaac ggcatcactg ttgatgacag cgtgtgggcc
    17701 aaaatccagg cgttatgagg agataagtca tgatttttgg acatatcgcg cagcccaatc
    17761 cgtgccgttt gcccgccgcc attgaaaagg cgctcgattt tctgcgcgct accaacttca
    17821 acgtcctgga gccgggcgtt gtcgaaatcg acggcaagaa tatttatgcg caaattatcg
    17881 atttaaccac acgcgaagcg gtggaaaacc gtccggaagt gcatcgtcgg tatatcgata
    17941 ttcagtttct ggcgtggggt caagagaaaa tcggcattgc tattgataca ggaaataata
    18001 aagtcagcga atcactatta gagcagcgcg atattatttt ttatcatgtc agtgaacatg
    18061 aatcatttat agaaatgata ccgggcagct acgctatttt cttcccgcag gatgttcacc
    18121 gaccaggttg tattctgcaa acaggcctct gagatacgaa aaattgtggt taaagtcgca
    18181 ttaacagcgc tgaattaatc aaataatcgt ctacgccaga acgactgggt aatgttattg
    18241 ctctaactat attaataatt taggttgctg cacatgcaac ttgaattatg acagacatag
    18301 cctcaaggaa tagctatgaa aaaaatactt gaagtaatac tggcgattaa tctcgccgta
    18361 ctttcctgta ttgtgtttat aaacattatt ttaagatatg gttttcagac aagcatttta
    18421 tctgtagatg aattgtcacg ttatttattt gtctggttaa catttattgg cgcgattgtg
    18481 gcttttatgg ataacgccca cgttcaggtc acctttctgg tggagaaact ctcccccgca
    18541 tggcaacgac gagtcgctct ggttacccat tccttaatct tatttatttg tggcgcactg
    18601 gcctggggcg caacactaaa aacaattcag gactggagcg attattcacc gatcctcggt
    18661 ttgcccatcg gcctgatgta tgccgcttgt ctgcccacca gcctcgttat cgcattcttt
    18721 gaactgcgtc atttatatca actcatcacg cgtagcaatt ctctaacgtc gccaccgcaa
    18781 ggagcttaat catggctgtg ctgatttttc tgggctgtct gttgggtggt attgctatcg
    18841 gtttgcctat tgcctgggca ctgttgttgt gcggggcggc gttaatgttc tggctggaca
    18901 tgtttgatgt ccagatcatg gcacaaacgc tggtgaacgg agccgatagc ttctccctgc
    18961 tggcgattcc gttctttgtg ctggcgggtg aaatcatgaa tgcgggcggc ttgtcaaaac
    19021 gcattgttga cctgccgatg aaactggtgg ggcataaacc aggcgggctg ggctacgtcg
    19081 gcgtgctggc agctatgatt atggcgagcc tttctggttc tgccgttgcg gataccgccg
    19141 ccgttgccgc tctactggtg ccgatgatgc gcagcgccaa ctacccggtc aaccgggcgg
    19201 cggggctgat tgcctctggc ggcattatcg cgccaattat tcccccttcc attccgttta
    19261 ttatcttcgg cgtttccagc ggattatcca tcagtaagct gtttatggcg ggcattgccc
    19321 ctgacatgat gatgggcgca acgctgatgc ttacctggtg gtggcaggcc agtcgcctta
    19381 atttaccccg ccagcaaaaa gcaacgatgc aggaaatctg gcactctttt gtctccggta
    19441 tctgggctct gtttcttcca gtcatcatta ttggcgggtt tcgctccggt ctgtttaccc
    19501 ctaccgaagc cggggccgtt gctgctttct atgcgctgtt tgtcgccaca gttatttacc
    19561 gtgaaatgac cttcgccaca ctttggcatg tactcattgg cgcggcgaaa accacctcag
    19621 tggttatgtt tctggtggcc tcggcacaag tttccacctg gctgattacc attgctgaac
    19681 tgccgatgat ggtttctgat ttactgcaac cgttggtcga ttcaccgcgt ctgctgttta
    19741 tcgtcattat ggtggctatc ctgattgtcg gcat
//
LOCUS       DAZPEB010000060        19728 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZPEB010000060 DAZPEB010000000
VERSION     DAZPEB010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19728
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.061"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..1629
                     /locus_tag="ACHURJ_001883"
     CDS             <1..1629
                     /locus_tag="ACHURJ_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFR1280851.1"
                     /translation="DQWDETPPVQRVTQSIDARLIQLTEKQNKTGGDFKEIDLLTRQL
                     KKLHDGQPDATATGKKGRAKKLKNHFTPEQIAALREKIISRLEWHQRGWFDSLTLCSE
                     AGIRNRMILKSRQIGATWYFAQEALLMALRDDVAQPYQRNQIFLSASRRQAFQFKSII
                     QKAAAEVDVELKGGDKIILSNGAELHFLGTSAATAQSYTGNFYFDEFFWVSRFAELRK
                     VAGAMATLSGLRRTYFSTPSTETHEAYVYWNGDRWNEKKAAHKRQRFSVDWKTLHNGL
                     ICPDRTWRQIVTLEDVVNHGWKHTDIDEIRDENTEDEFRNLYMCEFVREGESAFNLNI
                     LIGCGVDGYDDWKDWKPFAPRPMGNRPVWIGYDANGSSGNGDSGAVSVVVPPAVPGGR
                     FRTVETRRVQGLEFEEQARVIEEFTCRYNVEHIGIDATGGHGDAVYQIVKRFFPAAIP
                     YTFTLSSKRSLVLKMLQIMRAGRWEYDRAERELVAAFNAVRKVKTPGGFITYETDRAR
                     GISHGDLAWATMLAVINEPIGGEGENERFTVMEF"
     gene            1629..2675
                     /locus_tag="ACHURJ_001884"
     CDS             1629..2675
                     /locus_tag="ACHURJ_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001706445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR1280852.1"
                     /translation="MSRKNKKVRMSSRIDLADALRKESSLSAFTFDGPYRLTGHDLLD
                     NMYCADNGRWYETPVDWYGLARAARQTSWHQSALYFKRNVLLGCYIPHPLLSRQDFSA
                     LALDWFVFGNAFLELRSNMLGEPLKLRHALAKYMRRGSDLESWWYVQDGKDAFQFRPG
                     KVCHLMNPDINQEIYGMPEYLGALLSASLSHSADMFRKLYYDNGSHAGCIIYIGAAQV
                     NRESMDSLKETLQGARGGGAFKNVLIHAPNGGKEGVQILPFQQITAKDEFMNVKAASR
                     DDVLAAHRVPPQLMGAMPGEKSAFGDVEKAARVYAINELMPVMEAMKHINDWLGEEVI
                     RFNPYALLDTQPTS"
     gene            complement(3797..4108)
                     /locus_tag="ACHURJ_001885"
     CDS             complement(3797..4108)
                     /locus_tag="ACHURJ_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012907691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFR1280853.1"
                     /translation="MTTPTRRISFYLKPAAVKNEGEACAWLDSLTPEARKSGQRVAFL
                     AGLALLKTNPAEAYRLAAWADDEMLPVTQISSKKFEAQSAPVAKITSQMAGNIRALFP
                     E"
     gene            complement(4113..5072)
                     /locus_tag="ACHURJ_001886"
     CDS             complement(4113..5072)
                     /locus_tag="ACHURJ_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFR1280854.1"
                     /translation="MKICIDDGSTNIKLAWTENGERRNAISPNSFKSEWSAPFGGTQP
                     ANYMLDSVRYGFDPVSDRFVQTTDTQYQYSDVNVIAIHHALVKSGITPQEVDVVVTLP
                     LSEYFDTNAQPDMANINRKKANVMRPVEYQNGEAFTIRNVRVMPESIPAGFKALADMS
                     PFESLLIVDLGGTTLDVAKVQGQLAGISQVFCDPHVGVSLMADAVLSVMATNGMRTSH
                     HIANTIIEHRHDEAWLRQHIHNDAHYNSLMAVIREKEETLKQRVIRALAGFSGYGRVM
                     VVGGGAEIVAPAIREACGVNATFITDGVPQFALVNGLYAMNKE"
     gene            complement(5149..7971)
                     /locus_tag="ACHURJ_001887"
     CDS             complement(5149..7971)
                     /locus_tag="ACHURJ_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFR1280855.1"
                     /translation="MTTEYIRDWQQPRHAVGREGTGIPAPESALSSWLDAYQAENERR
                     QEKADAAFSATPLGNLINKSLDAQEKQDKTITLAGDARRQARGAVDEAMASLRLLPSY
                     LRDPLIRHLSFLRKKQEVDRRKGKKSWQAERYARGTLRKIFERLDRTDGHWLTPGYRS
                     LAGRERLDDLLYLPQLNKHQIQTLATMTAAMFSSTFEKLCDGFGATDGELTMDVTLKA
                     YQMLARMALHLHAMPPHYDALTTDKGRRNEPDTELLPGAILRLTCAEWWKRKLWLLRC
                     EWREEQLRAACLVSRKTSPYLSQDALSEFRAQREKTRDFLKSFMLENEDGFTIDLETV
                     YYAGVSNPVHRKAEMMATMKGLELLAEARGDRAVFLTVTCPSKYHATTENGHPNPKWN
                     GATMRDSSDYLVNSFFAAVRKKLNRDGLRWYGIRTVEPHHDGTVHWHMMVFAHPEEID
                     TIVSHTRDIAIQEDRHELGDDITPRFKAEYVDGSKGTPTSYIATYIGKNLDSRAVDGI
                     DPKTGKPRVDHETGKSMAESVERAIGWARLHRVRQFQFFGIPSRQVWRELRRLASQMA
                     RNPEGPQRLKDDAMDAVLAAADAGCFASYIEKQGGVLVPRQDYLIRTAYDLADELNDY
                     GEQSVQIYGIWSPLIGESSRVCTHPDNWKLVRRKPEAEDSARENGFDLQGGPAAPWTR
                     GNNCPRVQETDNNGTEQPEERPAPWPQLPDGVEVNEWMRSLKRHERRALMRSLRDKQA
                     KNSSDEMQNWTQSRKQPRPLPDNHELLAKEWRESAESLGLHIGEQQMQHLLRGGSLYV
                     DGSIIAPQGFEIVRKPDTRPDSRITQLWQHLSRNHGVSSTEIRHNPVASYLEQLGASD
                     PEAAARLASTLQQDQNTMKTPVTVLSDMLRAIRDAEHAQRISETTERARRKADLLRGG
                     LTSRNKKQTETGFTNPVNEQKTRRDI"
     gene            complement(7978..8343)
                     /locus_tag="ACHURJ_001888"
     CDS             complement(7978..8343)
                     /locus_tag="ACHURJ_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1280856.1"
                     /translation="MILANDFLEYLLNTERDLAVRVRERYDMYLKSLPVPQLADGKIV
                     IDGRYMIDSHEGNYRLYRIEGGTPSVIGIYQRPSSAIVDVIADSIRITHRYADTEDTV
                     LEIQRLAAVCRDTLNGMTQ"
     gene            complement(8416..8646)
                     /locus_tag="ACHURJ_001889"
     CDS             complement(8416..8646)
                     /locus_tag="ACHURJ_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="derepression protein"
                     /protein_id="HFR1280857.1"
                     /translation="MANRKQHRAIAERRHIQTEINRRLSRAFRVAKIMHINMLHERSC
                     ELSNLYSSAVFSYLADDLRELQQLIQQQNKLH"
     gene            complement(8694..9014)
                     /locus_tag="ACHURJ_001890"
                     /pseudo
     CDS             complement(8694..9014)
                     /locus_tag="ACHURJ_001890"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9014..9844)
                     /locus_tag="ACHURJ_001891"
     CDS             complement(9014..9844)
                     /locus_tag="ACHURJ_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFR1280858.1"
                     /translation="MKKFRLFQILPLFAAILLVGCDRVEPGNVGIKVNKLGDDKGVGE
                     VVGVGRYWTGWNTEVYIFPTFKQMKSYDEPFNFQMSDGTTIGYHIGVAYKVDPSKVTT
                     VFQTYRKGVDDITDTDLRQKIADALNRLASKMTTDKFIDGGKSELLDSALKDIQEEMT
                     PIGIQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQREQEVKQREAEANMLRAEAAG
                     QADAIRTKAQAEADAIRLRGEALRQNPGVMELEAINKWNGTLPQYMTSNTAVPFVPVK
                     "
     gene            complement(9841..10044)
                     /locus_tag="ACHURJ_001892"
     CDS             complement(9841..10044)
                     /locus_tag="ACHURJ_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1280859.1"
                     /translation="MNKKTWFRAYMWALVCVLVSLILYAGLLPRMISSDSSFLVLLGI
                     FIAMLYPAGVVRLFSKYIKEIKQ"
     gene            complement(10056..10355)
                     /locus_tag="ACHURJ_001893"
     CDS             complement(10056..10355)
                     /locus_tag="ACHURJ_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1280860.1"
                     /translation="MSAINHQELRELATDLQRMATHQKLLAFRAMLSPSAVLALLDEL
                     EHARTMAPAIRLTLHHEIKDFCATLESPGESETPEAMQQELLQRIDNVFDFFLNQ"
     gene            complement(10352..10618)
                     /locus_tag="ACHURJ_001894"
     CDS             complement(10352..10618)
                     /locus_tag="ACHURJ_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFR1280861.1"
                     /translation="MTILDYIATHPGCSGGEIAAALNTPTTAINAELRRLWRGGLVIR
                     TNRSTGGRARKAGGQASYHVNPMPFGCSNPLTHMFNLLLKEARA"
     gene            complement(10615..10818)
                     /locus_tag="ACHURJ_001895"
     CDS             complement(10615..10818)
                     /locus_tag="ACHURJ_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1280862.1"
                     /translation="MAIKHFPVVRFTSRDREYEVDERLITTIDKHRSEKDAHHIYLTD
                     GTYFCATNVVRVNLIRQVQESRR"
     gene            complement(10818..11135)
                     /locus_tag="ACHURJ_001896"
     CDS             complement(10818..11135)
                     /locus_tag="ACHURJ_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1280863.1"
                     /translation="MKTIRTRGGIHIYKHEVSMLRTNQKILKDAYFDRGRDLAKLCAA
                     LKEISQYFTDIEDDIANKGASITLANRAHFMGFNVFTAIRTLKEQKEEIEKLKYRHKA
                     KRV"
     gene            complement(11171..11284)
                     /locus_tag="ACHURJ_001897"
     CDS             complement(11171..11284)
                     /locus_tag="ACHURJ_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1280864.1"
                     /translation="MSIFISWLVLIISVACAIGIMRIIHSVKKIERFFTEE"
     gene            complement(11281..11523)
                     /locus_tag="ACHURJ_001898"
     CDS             complement(11281..11523)
                     /locus_tag="ACHURJ_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HFR1280865.1"
                     /translation="MGKEYKTLINKALERFYFRLSASGAHAERAARDSLTRAIRSLYD
                     VAFYADDLDALNELSELICAAECGEHIEPYKLGNIA"
     gene            complement(11535..11822)
                     /locus_tag="ACHURJ_001899"
     CDS             complement(11535..11822)
                     /locus_tag="ACHURJ_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1280866.1"
                     /translation="MTKEHAQGVFIRFIDFRGELLLRVSAIDGVTPAGKNGAAEATYI
                     YLNGTRLLVELPYQTVREIISEAEKARQVNGDEPYIEIICMDSEAEIQKAD"
     gene            complement(11833..12174)
                     /locus_tag="ACHURJ_001900"
     CDS             complement(11833..12174)
                     /locus_tag="ACHURJ_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFR1280867.1"
                     /translation="MKQQRNSRFRNGAERHANRFATSASRSNIRYSLSDTHATPDGYP
                     AKQIGEHAWLIEKAGIVVHKCPRNPFTGNRIFALSSGDNQFGQDFTLYEALRTVDRLL
                     RGQSFIKQADL"
     gene            complement(12193..12519)
                     /locus_tag="ACHURJ_001901"
     CDS             complement(12193..12519)
                     /locus_tag="ACHURJ_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cox family DNA-binding protein"
                     /protein_id="HFR1280868.1"
                     /translation="MNEELLRALFKIPDPITVDEFARRTGKTESAVRKLVERRLIPLA
                     TEREVLGEEGSSRRLLILWNEWLEMVYDATKQLPPERKDWRNHWLNKAKKLAEDLGLG
                     FLNFAA"
     gene            12614..12916
                     /locus_tag="ACHURJ_001902"
     CDS             12614..12916
                     /locus_tag="ACHURJ_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001242988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1280869.1"
                     /translation="MILEIGEKLKLMRESERITSRNEAAELIGIPHNALWRYETGESI
                     PKGDVMMKILNCPRFEKYALWFMTGKIAPESGQVAPALAHYGQEPTDLPPSERKIG"
     gene            12983..13972
                     /locus_tag="ACHURJ_001903"
     CDS             12983..13972
                     /locus_tag="ACHURJ_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003805948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1280870.1"
                     /translation="MAIKALDGGRYKVDVRPRGRSGRRIQRIFKKKADAVAFERYVLS
                     HMHDKEWLEKPTEQRHLSDLLPLWWELGGRNKPYANGVLTRLKKIIKEMNDPRVSQIN
                     ARFMAAYRSSRLSLGVKESTVRRDESDLGGMFTLLANAGEFHGENPLRALPSLKRKSP
                     EMTYLTTEEIAKLLDAVSGDARRITLLCLSTGARWGEAKNLRAEHIINNRVTFNKTKN
                     GKVRIIPVSDEVVSEIKTKKSGLLFDVNYEEYRKVLRSVKPDLPKGQAVHVLRHTFAA
                     HFMINGGNILTLQRIMGHATIQQTMTYAHLAPDFLQDAISLNPLKGGIHISST"
     misc_feature    14139..14264
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            14140..14187
                     /gene="pheL"
                     /locus_tag="ACHURJ_001904"
     CDS             14140..14187
                     /gene="pheL"
                     /locus_tag="ACHURJ_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFR1280871.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            14287..15447
                     /gene="pheA"
                     /locus_tag="ACHURJ_001905"
     CDS             14287..15447
                     /gene="pheA"
                     /locus_tag="ACHURJ_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFR1280872.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(15490..16611)
                     /gene="tyrA"
                     /locus_tag="ACHURJ_001906"
     CDS             complement(15490..16611)
                     /gene="tyrA"
                     /locus_tag="ACHURJ_001906"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFR1280873.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLKQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(16622..17692)
                     /gene="aroF"
                     /locus_tag="ACHURJ_001907"
     CDS             complement(16622..17692)
                     /gene="aroF"
                     /locus_tag="ACHURJ_001907"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFR1280874.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDLETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVGQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            17900..18265
                     /locus_tag="ACHURJ_001908"
     CDS             17900..18265
                     /locus_tag="ACHURJ_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFR1280875.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFGDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVESASQ
                     LHEVWQKGADENASTIRLN"
     gene            18415..18933
                     /locus_tag="ACHURJ_001909"
     CDS             18415..18933
                     /locus_tag="ACHURJ_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFR1280876.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAKQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            18923..>19728
                     /gene="dgcN"
                     /locus_tag="ACHURJ_001910"
     CDS             18923..>19728
                     /gene="dgcN"
                     /locus_tag="ACHURJ_001910"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFR1280877.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINT"
BASE COUNT         4696 a         4995 c         5007 g         5030 t
ORIGIN      
        1 gaccagtggg atgaaacgcc acccgtgcag cgtgtcacgc agtccatcga tgcgcgcctc
       61 atccagctta ctgaaaaaca gaataaaaca ggtggtgact ttaaggaaat agacctgctg
      121 acccggcagc ttaaaaaact gcatgatggc cagccggatg cgacggccac aggaaagaaa
      181 ggccgggcga aaaaacttaa aaatcatttc acgccggagc agattgccgc actgcgggaa
      241 aaaatcatca gcaggctgga gtggcatcag cggggctggt ttgactccct gaccctttgc
      301 agtgaagccg ggatacgtaa caggatgatc ctgaaatccc gacagattgg ggcgacctgg
      361 tattttgcac aggaagcact gctgatggcg ctgcgtgacg atgtggcaca accttaccag
      421 cgtaaccaga tttttttgtc tgcgtcgcgt cgtcaggcgt tccagtttaa aagcattatt
      481 cagaaggccg cggctgaagt tgatgtggag ctgaaagggg gcgataaaat catcctctcc
      541 aacggcgcag aactgcattt tctcggtact tctgctgcga cggcacagtc ctacacaggt
      601 aatttttatt ttgatgaatt tttctgggtc agtcgctttg ctgaactgcg caaggtggct
      661 ggcgctatgg caaccctcag cggactgcgg cgcacctact tctccacgcc atccaccgaa
      721 acgcacgagg catacgtcta ttggaacggc gaccgctgga acgagaaaaa ggccgcgcat
      781 aaacgccagc gtttttctgt ggactggaaa acgctgcata acggacttat ctgccccgac
      841 cggacgtggc ggcaaattgt cacgctggaa gatgtggtta atcacggctg gaaacacacc
      901 gatattgatg aaattcgtga tgaaaacacc gaagacgagt tccgcaatct ctatatgtgt
      961 gagtttgtcc gcgaagggga atcggcattt aacctgaata tcctgattgg ctgcggtgtt
     1021 gatggatacg acgactggaa agactggaaa ccttttgctc cccgcccgat ggggaatcgt
     1081 ccggtatgga ttgggtatga cgcaaacggc agcagtggaa acggcgacag cggcgcggtg
     1141 tccgtggtgg ttcctccggc tgttcctggt ggccgttttc gaacggtgga gacgcgacgc
     1201 gttcaggggc tggagtttga agaacaggcc agagtcattg aagagttcac gtgtcgctac
     1261 aacgtggaac acatcggcat tgatgcgact ggcgggcacg gggatgccgt ttatcagata
     1321 gtgaaacggt ttttccctgc tgctattccg tacaccttca cgctgtcatc aaaacggtcg
     1381 ctggtactga aaatgctgca aataatgcgt gccgggcggt gggaatacga tcgtgccgaa
     1441 cgcgagctgg tcgcggcctt taacgccgtg cgtaaggtga aaacaccggg cggctttatc
     1501 acttacgaaa cggaccgagc gagggggatc agccacggcg accttgcgtg ggcaaccatg
     1561 cttgctgtca ttaatgaacc gattggcggc gaaggagaaa acgagcgttt cacggttatg
     1621 gagttctgat gagcagaaaa aataaaaaag tgcgcatgag ttcacgcatt gatctcgctg
     1681 atgcgctcag gaaagaatca tcgctcagtg cattcacatt tgatggtcct tatcgcctga
     1741 ccgggcatga cctgctggac aatatgtact gtgctgataa cgggcggtgg tatgaaaccc
     1801 cggtggactg gtacggtctg gcaagagctg cccggcaaac gtcctggcat cagtctgcgc
     1861 tttactttaa gcgcaatgta ttgctcggct gctatattcc gcacccgctg ctttcccggc
     1921 aggatttctc ggcgctggcg ctggactggt ttgtgttcgg taacgcattc cttgagcttc
     1981 ggagcaatat gctcggcgaa ccgcttaaat tacggcacgc cctggcgaaa tacatgcgac
     2041 gcggaagcga tcttgaatca tggtggtatg tgcaggatgg caaggacgcg tttcagtttc
     2101 gtcctggcaa agtgtgccac ctgatgaatc cggatattaa ccaggaaatc tacggcatgc
     2161 cggaatatct tggcgcatta ctctcggcca gcctttctca ttcggcggac atgttcagaa
     2221 aactgtacta cgacaacgga tcccacgccg ggtgcatcat ctacatcggt gcagcgcagg
     2281 taaaccgcga aagcatggac tccctgaaag aaacgctaca gggggcacgt ggtggtggtg
     2341 cgtttaaaaa cgtgctcatt catgcgccca acgggggcaa agagggggtg caaattttgc
     2401 cgttccagca gatcaccgca aaggatgagt tcatgaatgt taaggcggca tcccgtgatg
     2461 atgtgctggc tgcgcaccgc gttccgccgc aactgatggg ggcgatgccg ggcgaaaaaa
     2521 gtgcgtttgg tgatgtggag aaggccgcgc gggtttacgc aattaacgag ctgatgcccg
     2581 tcatggaggc catgaagcac atcaatgact ggcttggcga agaggtgatc cgctttaacc
     2641 cttacgcact gttagatacc cagcccacat cctgacgcgc ttcgcttgtc tgctgcttcg
     2701 ccggggcata aaaaatttat gccccgactc tccagctcct gtatcagtca gataatttca
     2761 cgacgctttc ctgcttattg ccatcatcga cggtcagatt cttacgcaat cccactgcgc
     2821 tgactgcatg ttctcgccgc ctcagtgcga ttttgacggc cttacctttc accccatcaa
     2881 accagaatcc ctcacgtatt tttcacgctc agcgtgagaa atacggccat tctgtcgtgt
     2941 cgctgcgaca tcgttaaggg aacgctattt accccctgaa acgcgggctg ttcccccgtc
     3001 acctgcgcgc agaaaaaacg catttttttg tgcacgcacg gatccttgac ggatccagcc
     3061 gccatgcggg ccggaagtac aaaaagtcgt tcaaaaaaat tgtgcaaact tgtgcactat
     3121 cgtgcaaaca aaaaaaagcg ccttatcggc gcttcaaaag tatcaattgt tgctgtgtat
     3181 taatcgccaa ccacgaacat atgcttcata ggcatctctg tgccttacag ttccagcctg
     3241 gctaaacgga atgttagcta aaactaaatc attctgagcc atagcgcgtc cttcaagcgc
     3301 atcttttatg cctagctcaa aagcacaggc agcgcatttg tgccgacctt cttgtccttg
     3361 atattcaggg agagacagaa atgttgggtt ataacgatga gggttcttgc aaatacctgt
     3421 tttagcccgc actttattta cctcatagga aaaatatgcg tgcctttaca gaggtgcgtg
     3481 taagcaataa aatatacgac gcacattttg atttacttcg gaaggcacgc atatcaggtt
     3541 aagtgagtcc gaccgagttt tacgccccaa tagttgctgc tattggggcg ttttgcatgg
     3601 acaatgccgc gcaattatct tgtcgctcac aatgcgaacg atcttacaaa aaggcacatt
     3661 actgtcaaga taattgatcg ttttaatcga tagataatag acaatctatt tgtttaacag
     3721 atcgattatt gaagtaagtg tgccaaatgg aatgatactg tttctataaa cgtgagcatt
     3781 tttcgtgcag atgcttttac tcaggaaata acgcccggat attcccagcc atctggctgg
     3841 ttatcttagc cactggtgca gactgtgctt caaacttttt tgagctgatt tgtgtcacag
     3901 gtaacatctc atcatcagcc catgcggcca gtcggtaagc ctctgccgga ttcgtcttca
     3961 gaagtgccag cccggccaga aaagccacgc gttggccgct tttgcgggct tctggtgtaa
     4021 ggctgtccag ccaggcgcat gcttctcctt cgttcttgac ggcagctggc ttcagataga
     4081 aacttattct tctggttggt gtcgtcattg gtttactcct tgttcattgc gtacagccca
     4141 ttaaccagag caaattgtgg caccccgtcc gtgatgaaag tcgcattaac tccgcaggct
     4201 tcgcggatag cgggtgccac aatctccgcc cctccaccga caaccatcac ccgcccgtaa
     4261 ccggaaaaac ccgccagcgc gcggatcact cgttgtttca gtgtctcctc cttttcacga
     4321 ataaccgcca tcaggctgtt gtaatgcgcg tcattgtgaa tgtgctggcg cagccaggct
     4381 tcatcgtgac ggtgctcaat aatggtattg gcgatgtggt gactggtacg cataccgtta
     4441 gtggccatca ccgacagtac ggcatcggcc atcagggaaa cgcctacgtg tggatcgcaa
     4501 aacacctggc tgatacctgc cagttgtccc tgaacctttg ccacatccag cgtggttccg
     4561 cctaaatcca caatcagcag ggattcaaac ggactcatgt cagccagtgc cttaaagcca
     4621 gccggaatgg attcaggcat aacccgtaca ttacggatag tgaatgcttc gccgttctgg
     4681 tattccaccg ggcgcataac gttcgctttt ttgcggttga tgttggccat gtccggctgt
     4741 gcgtttgtgt cgaaatactc gctcagtggc agggtgacaa ccacatccac ctcctgtggc
     4801 gtgatgcctg atttgaccag cgcgtgatga atggcaatga cattcacatc actgtactga
     4861 tattgcgtgt cagtcgtctg gacaaagcga tcgctgaccg gatcaaaacc atagcgcacg
     4921 ctatcaagca tgtagttcgc gggctgcgtg ccaccgaacg gcgcagacca ttccgacttg
     4981 aagctgttcg ggctgatggc gttgcggcgt tcgccgttct cagtccatgc cagcttgatg
     5041 ttggtggagc cgtcgtcgat acaaattttc atgtcgattt tccttatgtt gattaattaa
     5101 tcgtttacgg gatttttaaa tcccgttttc gcctgttttg tgcgcgcttc atatatcgcg
     5161 gcgcgttttt tgctcattta cgggatttgt gaatcccgtt tctgtctgtt ttttgtttct
     5221 actggtcagg ccaccccgca gcaggtctgc tttgcggcgg gcgcgttcag ttgtttcact
     5281 gattctctgt gcgtgctctg cgtcacgaat ggcgcgcagc atgtcagaaa gcacggtaac
     5341 gggggttttc atggtgttct ggtcctgctg aagtgtggat gccagacgtg cggcggcttc
     5401 ggggtctgat gcccccagct gttccagata gctggcgacc gggttatggc ggatctccgt
     5461 gctgcttacg ccgtgattac ggctcaggtg ctgccagagc tgcgtgattc ggctgtccgg
     5521 gcgggtatcc ggtttgcgta caatttcaaa tccctgtggt gcaatgatgc tgccgtcaac
     5581 gtacaggctg ccgccccgta acaggtgctg catctgctgt tcaccgatat gcaggccgag
     5641 agattcggca gattcccgcc attctttagc gagtaattcg tggttatcag gcaaaggccg
     5701 tggctgtttg cggctctgtg tccagttctg catttcatca ctgctgtttt ttgcctgctt
     5761 gtcacgcagc gaacgcatca gcgcccggcg ttcgtgccgt ttcagtgagc gcatccattc
     5821 gttcacttca acgccgtcag ggagctgcgg ccacggtgct ggccgttctt ccggctgttc
     5881 tgtcccgttg ttgtccgttt cctgtacacg gggacagtta ttgccacgag tccaaggggc
     5941 ggcagggccg ccctgaaggt caaaaccatt ttcgcgggcg ctgtcttccg cttccggttt
     6001 acgtcttacc agcttccagt tatccggatg tgtgcacaca cgggaagact ccccgatgag
     6061 tggtgaccag atcccgtaaa tctgtacgct ctgttcgccg taatcgttca gctcatctgc
     6121 gaggtcgtag gcggtgcgaa tcaggtagtc ttggcgtgga acaagtacgc cgccctgttt
     6181 ctctatgtag gaggcaaaac acccggcatc agcggcagcg agcactgcat ccattgcgtc
     6241 atccttcagc cgttgcgggc cttccgggtt gcgtgccatc tggctggcaa ggcggcgcag
     6301 ttcacgccac acctgacggg aggggatgcc aaagaactgg aactggcgga cccggtgaag
     6361 gcgcgcccag ccgatggcgc gctccacgct ctcggccatt gattttccgg tttcgtggtc
     6421 aacgcgtggc ttgcccgttt tcgggtcgat accatccacg gcgcggctgt ccaggttctt
     6481 tccgatgtag gtggcgatgt agctggtcgg tgtgcctttt gagccgtcga cgtactctgc
     6541 cttaaagcgt ggggtaatat catcacccag ctcgtgacga tcttcctgaa tggcaatatc
     6601 gcgggtgtgg gacacaatgg tgtcgatttc ttccggatga gcaaagacca tcatatgcca
     6661 gtgcacggtg ccgtcatggt gaggctccac cgtgcggatg ccataccagc gcaggccgtc
     6721 gcggttcagt ttcttgcgga ccgccgcaaa aaacgagtta accaggtaat cgctggagtc
     6781 gcgcatagtg gccccgttcc atttgggatt cggatgaccg ttctccgttg ttgcgtggta
     6841 ttttgacggg caggtgacag tcagaaacac cgctctgtcg ccacgggctt cggccagaag
     6901 ttccagcccc ttcatggtgg ccatcatttc tgccttacgg tgaaccgggt tacttactcc
     6961 cgcgtaatac actgtctcaa gatcaatcgt gaacccgtct tcgttttcca gcatgaaact
     7021 tttcaggaaa tcgcgtgttt tctcgcgctg tgcgcgaaac tcgcttaacg catcctggct
     7081 cagatagggc gatgtttttc tggaaaccag acaggcggcg cggagttgtt cttctctcca
     7141 ctcgcaacgt aacagccaca gtttgcgttt ccaccattcc gcacaggtca ggcgaaggat
     7201 tgcgcccggc agcagctccg tgtctggttc gttcctccgg cctttgtctg ttgtcagtgc
     7261 gtcataatgc ggaggcatgg cgtgcaggtg taacgccatg cgggccagca tctgatacgc
     7321 cttcagcgtt acatccatgg tcagctcgcc atcagtcgca ccaaagccat cgcagagttt
     7381 ttcgaaggtg ctgctgaaca tcgccgccgt catggtggcc agcgtctgta tctggtgttt
     7441 gttgagctgc ggcaggtaaa gcaaatcgtc caggcgttcg cgtccggcaa gggagcgata
     7501 acccggtgtc agccagtgtc cgtcagtgcg atccagacgt tcgaatattt tgcgcagggt
     7561 tccgcgtgca tagcgttctg cctgccagct ctttttgcct ttccggcgat cgacttcctg
     7621 ttttttgcgc aggaaggaga ggtggcgaat aagcggatcg cgcagatagg acggcagcag
     7681 gcgcagcgag gccatggctt catccaccgc gccgcgtgcc tgtcttctgg cgtctcctgc
     7741 cagcgtgatg gttttgtcct gtttttcctg tgcgtccagg cttttattaa tcaggttgcc
     7801 cagtggcgtg gcagagaacg ccgcatcagc cttttcctgt cggcgctcgt tctctgcctg
     7861 gtaggcatcc agccaggagg aaagcgcgga ttcaggtgcg gggatccccg ttccttcacg
     7921 ccccactgcg tggcgcggtt gttgccagtc cctgatgtac tctgtcgtca tactgattta
     7981 ctgcgtcata ccgttcaggg tgtcacggca aacggcagcc agccgctgaa tttccagcac
     8041 ggtgtcttct gtgtcggcat agcgatgtgt gatgcggatg ctgtcggcaa tcacatcgac
     8101 gattgcagag gatgggcgct ggtaaatgcc aataacggac ggagtgccac cttcaatgcg
     8161 gtaaagcctg taatttccct cgtggctgtc aatcatgtag cgaccatcaa taacaatctt
     8221 tccgtcagcg agctgcggta caggcaggga tttcaggtac atgtcataac gttcacgcac
     8281 gcgaacggca agatcacgct ctgtgttgag caggtattca agaaagtcgt tggcgagaat
     8341 cattgcggca atcctcttgt tacagatgtg cgaaggcctc ctgccgcaag gtgcaggaaa
     8401 ggcccggaac aggaattaat ggagtttgtt ttgctgctgg atgagctgct gaagctcgcg
     8461 cagatcatcc gccagataac tgaaaacagc ggatgagtag aggtttgaaa gttcgcagct
     8521 acgctcatgc agcatattga tgtgcatgat tttagcgacg cggaatgcgc gggaaagtct
     8581 gcggttgatt tcagtctgga tgtgacgacg ctccgcgata gcgcggtgct gtttgcggtt
     8641 tgccatggtg tggcctcttt ggttgtaagt tttgaaaact caccatccag agctgcgaaa
     8701 ctgtgggtgg cgagacgtac ggggttcgca gtaccggcaa ccaaagaacc cggcccgacc
     8761 gaagtcggcc ccgtacgccc cgccataatt ctgacgcgaa aaaaacgtgg caatacagta
     8821 cgcacaaaaa aaccgctggc gcggttgtgc gctttggttg tcagcaggct gcgaaacccg
     8881 gcacccgttt tatgaggtgc agcgaaaatg taacctgact gattgcggca tggcaagcgg
     8941 tttttttgtg agaacggcat actaaaaaat cctgatactg ctccggccag cggtttgcac
     9001 tggccgggtg tcattacttc acgggaacga acggaacagc ggtgttactg gtcatgtatt
     9061 gcggcagcgt gccgttccat ttgttgattg cttccagctc cataacgccg ggattctggc
     9121 gcagagcttc gccgcgtaaa cgaatggcgt cggcttcagc ctgggctttt gtgcgaatgg
     9181 catcggcctg tccggcagct tctgcgcgca acatgttggc ttctgcttcg cgctgcttga
     9241 cttcctgttc gcgttgcagg gttttctggt ttgccgtgac tttggcgtta atgctgtcga
     9301 taacggttgg cggatattcc ggtttaccga cataagagag gctcatgacc tgaataccga
     9361 tgggggtcat ctcttcctga atatctttaa gtgctgaatc cagcagttca gacttaccac
     9421 cgtcgataaa cttatcggtg gtcattttgc tggccagtcg gttgagtgca tcggcgatct
     9481 tctggcgcag gtcagtgtcg gtaatgtcgt ccacgccttt gcggtaggtc tgaaacaccg
     9541 tggtaacttt ggatggatca actttgtagg ccacgccgat gtgatagccg atggttgtgc
     9601 cgtcactcat ctggaagttg aacggctcat cgtaactctt catttgtttg aaggtcggga
     9661 agatgtaaac ctctgtattc cagcctgtcc agtagcggcc aacgccaacc acttcaccga
     9721 cgcctttgtc gtcgcccagt ttgttgactt tgatgcccac attacctggc tcaacgcgat
     9781 cgcaaccaac cagcaggatg gcggcaaaaa gcgggagaat ctgaaagagt ctgaatttct
     9841 tcattgtttg atttccttga tgtacttact gaaaaggcga acaacgcctg ccgggtacag
     9901 catggcaatg aaaattccca gcaataccag gaaggagctg tctgatgaaa tcattcgggg
     9961 gagtagtcct gcatacagaa tgagagagac gaggacgcat accagcgccc acatgtatgc
    10021 gcgaaaccag gtctttttgt tcatatcgcg gtcctttact ggttaaggaa aaaatcgaaa
    10081 acgttgtcga tgcgttgcag cagctcctgc tgcattgctt ccggcgtttc tgattcacct
    10141 ggtgactcca gcgtcgcgca aaaatccttg atttcatgat ggagcgtcag acgaatggca
    10201 ggagccatgg ttctggcgtg ctccagctca tccagcagtg ccagcacggc agatggcgag
    10261 agcattgcgc gaaacgccag taatttttga tgcgttgcca ttcgttgcag gtcagtcgcc
    10321 agttcgcgta attcctggtg gttgatggcg ctcatgctct ggcttccttc agtagcaggt
    10381 taaacatgtg agtaagtgga ttgctacacc cgaacggcat cgggtttacg tggtaagaag
    10441 cctggcctcc tgctttgcga gcgcgaccac ctgtgctgcg gtttgttctg atgactaagc
    10501 cgccgcgcca gagtcggcgt aactcagcat tgatggctgt ggttggggta ttcagtgctg
    10561 cggcgatttc tccgccgcta caacccggat gggtagcgat gtagtccaga atggtcatct
    10621 gcgtgactcc tgtacctgtc ggataaggtt cacccgcacc acattcgtgg cgcagaagta
    10681 agtgccgtca gtgagataga tgtgatgtgc atccttttcc gaacgatgtt tgtcgatagt
    10741 ggtaatcagt cgttcgtcga cctcgtattc gcggtctctg gaggtgaagc gaacgacagg
    10801 aaaatgctta attgccatta caccctcttg gctttatgtc tgtatttgag cttttctatt
    10861 tcttcttttt gttcttttaa tgttcttatg gctgtgaaaa cattaaagcc cataaaatgt
    10921 gctctgttcg ctaatgtaat actggcacct ttgttagcta tatcatcttc aatatcagtg
    10981 aaatactgac tgatttcttt tagtgcggcg caaagctttg cgagatctcg ccccctgtca
    11041 aaatatgcat ccttcagtat tttttgattt gtcctgagca tgctaacctc gtgtttatat
    11101 atatggatac ctccgcgagt gcggattgtt ttcatgtttt cttatttaat cgtgtgtttt
    11161 atttgtgctg ttattcttca gtgaaaaaac gctcaatctt ttttactgaa tgaataattc
    11221 gcataatccc aatagcgcag gccaccgaaa taatcagaac aagccatgag ataaatatac
    11281 tcatgcgata ttccccagct tatacggttc aatatgttcc ccgcattctg cggcacagat
    11341 cagctcggaa agttcgttaa gtgcatccag atcatcagcg taaaaagcca cgtcatacag
    11401 acttcggatt gctctggtca atgagtcacg ggccgcacgt tcagcatgag cgcctgatgc
    11461 acttaagcga aaataaaaac gctcaagtgc tttgttaatg agagttttat attctttgcc
    11521 catcacaacg ccctttaatc tgctttctgt atttcagctt ctgaatccat acaaataatt
    11581 tcgatatagg gttcatcgcc attaacctgg cgtgcctttt cagcttcgct aatgatttct
    11641 cgtacggtct ggtacggaag ttccacaagc agccgtgtgc cgttcagata aatgtaagtg
    11701 gcttcagcgg ctccgttttt acccgccgga gtcactccat caatagcgga tacgcgtaat
    11761 aacagttcac cgcgaaaatc aataaaacgg ataaatacac cttgtgcatg ctctttggtc
    11821 ataaagcacc tgttataaat cagcctgttt aataaaactt tgcccacgaa gcagacgatc
    11881 aaccgtgcga agtgcttcgt ataatgtgaa atcctgtccg aactgattgt cgccgctgct
    11941 tagtgcaaaa atgcggtttc cggtaaacgg attgcgtggg catttgtgga ctacgattcc
    12001 agctttctca atcagccagg catgctcgcc gatttgtttt gctgggtagc catccggtgt
    12061 tgcgtgtgta tcactcaggc tgtagcggat gttgctgcgt gatgcactgg tagcgaaacg
    12121 gttggcgtgg cgttcagcac cattacgaaa gcgtgaatta cgttgctgtt tcatatcaaa
    12181 actccctgca tctcatgcag caaaattaag aaagcctaat cccaaatctt ccgccagctt
    12241 cttggcttta ttaagccagt gattgcgcca atctttacgc tcaggaggaa gttgtttcgt
    12301 tgcgtcatag accatttcga gccactcgtt ccataggatg agaaggcggc gtgaacttcc
    12361 ttcctcgcct aaaacttcac gctcagtggc cagagggatg agccgacgtt ccaccaactt
    12421 tcttacggct gattcggtct tgcccgtgcg gcgggcaaat tcatcgacgg tgatggggtc
    12481 tgggatctta aacaatgccc tcaatagttc ttcattcatg tgataatctc cctgtttggg
    12541 gtataggtga gatatttacc ccatagttca actcattgtt tgcataatgc actacatatt
    12601 tggagaatgc aatatgattt tggagattgg tgaaaaacta aaactaatgc gtgaaagtga
    12661 gcgcattaca agtcgcaatg aggctgcgga gttaataggt attccgcata acgcgctatg
    12721 gcgttatgag actggcgaat ccatacccaa aggggatgta atgatgaaaa tcttaaattg
    12781 cccgcggttc gaaaaatatg ccttgtggtt tatgactgga aaaattgcgc ctgaatccgg
    12841 gcaggtagct ccggctctcg cacactatgg gcaagagcca acggacttac ccccatccga
    12901 aaggaaaatt ggttaaccct ttattattct tacgttttac aaactgggaa tgtctttcct
    12961 cgtttcaccg gagggcttgc caatggcaat taaggcactc gatggtggac ggtataaagt
    13021 ggatgttaga ccgcgtggcc gaagtggacg tcggattcag cggattttta agaaaaaggc
    13081 agatgcagtg gcctttgagc gctatgttct cagccacatg cacgataagg aatggcttga
    13141 aaagccaaca gagcaacgtc atctctcaga tctgcttcca ttatggtggg aattgggtgg
    13201 acgcaataag ccatatgcta acggcgttct aaccagattg aaaaaaatca tcaaagaaat
    13261 gaatgatcca agggttagcc agattaatgc tcgtttcatg gcagcttatc gaagctcccg
    13321 tttatcctta ggagtaaaag aatctactgt tcggcgtgat gagtcggatc tcggaggaat
    13381 gtttacactc ctggcaaatg ccggggaatt tcacggagaa aatccactcc gcgccctccc
    13441 ctctttgaaa cgaaaatcac ccgaaatgac gtacctcacc acggaagaaa tcgccaaatt
    13501 gctggatgca gtaagcggtg atgcccggcg gattacgcta ctttgtctca gtactggggc
    13561 gagatgggga gaagcgaaaa atctgcgcgc ggaacacatc atcaataatc gcgtgacgtt
    13621 taacaaaact aaaaacggaa aagttcgaat tattcctgtc tctgatgagg ttgttagtga
    13681 gatcaaaaca aagaaatccg gccttttgtt tgacgtcaat tatgaggaat atcgcaaggt
    13741 gcttcgcagt gttaagccag acctaccaaa gggacaggct gtacatgttc tacgccatac
    13801 ctttgcagct cactttatga ttaatggagg aaatatactt acgctccagc gaattatggg
    13861 gcacgccacg attcagcaaa ctatgaccta tgcgcacctc gctcctgatt tcctccagga
    13921 tgcaatttca cttaatccgt taaaaggagg catccacatt tcatccacat aagtgggttt
    13981 tatagggtaa tcagtaactc ccaccaataa caaaacccac tgaaaacaaa gggaaaacat
    14041 tgaatttagt ggggtttaca acgcaccttc gggcgcgttt tttgttgaca gcgtgaaaac
    14101 agtacgggta ctgtactaaa gtcacttaag gaaacaaaca tgaaacacac accgtttttc
    14161 ttcgcattct tttttacctt cccctgaatg ggaggcgttt cgtcgtgtga aacagaatgc
    14221 gaagacgaac aataaggcct cccaaaccgg ggggcctttt ttattgataa caaaaaaggc
    14281 aacactatga catcggaaaa tccattactg gcgctgcgag agaaaatcag cgcgctggat
    14341 gaaaaattat tagcattact ggcagagcgg cgcgaactgg ccgtcgaggt gggaaaagcc
    14401 aaactgctct cgcatcgccc ggtacgagat attgatcgtg aacgcgattt actggaaaga
    14461 ttaattacgc tcggtaaagc gcaccatctg gacgcccatt acattactcg cctgttccag
    14521 ctcatcattg aagattccgt attaactcag caggctttgc tccagcaaca tctcaataaa
    14581 attaatccgc actcagcacg catcgctttt ctcggcccca aaggttctta ttcccatctt
    14641 gcggcgcgcc agtatgctgc ccgtcacttt gagcaattca ttgaaagtgg ctgcgccaaa
    14701 tttgccgata tttttaatca ggtggaaacc ggccaggccg actatgccgt cgtaccgatt
    14761 gaaaatacca gctccggtgc cataaacgac gtttacgatc tgctgcaaca taccagcttg
    14821 tcgattgttg gcgagatgac gttaactatc gaccattgtt tgttggtctc cggcacgact
    14881 gatttatcca ccattaatac ggtctacagc catccgcagc cattccagca atgcagcaaa
    14941 ttccttaatc gttatcctca ctggaagatt gaatataccg aaagtacgtc agcggcaatg
    15001 gaaaaggttg cacaggcaaa atcaccgcat gttgctgcgt tgggaagcga agctggcggc
    15061 actttgtacg gtttgcaggt actggagcgt attgaagcga atcagcaaca aaacttcacc
    15121 cgatttgtgg tgttggcacg taaagccatt aacgtgtctg accaggttcc ggcgaaaacg
    15181 acgttgttaa tggcgaccgg gcaacaagcc ggtgcgctgg ttgaagcgtt gctggtgctg
    15241 cgcaaccaca atctgattat gacccgtctg gaatcacgcc cgattcacgg taatccatgg
    15301 gaagagatgt tttatctgga tattcaggcc aatcttgaat cagcggaaat gcaaaaagca
    15361 ttgaaagagt taggtgaaat tacccgttca atgaaggtat tgggctgtta cccaagtgag
    15421 aacgtagtgc ctgttgatcc aacctgatga aaaggtgccg gatgatgtga atcatccggc
    15481 actggattat tactggcggt tgtcattcgc ctgacgcaat aacacgcggc tttcactctg
    15541 aaaacgctgt gcgtaatcgc cgaaccagtg ctccaccttg cggaaactgt caataaacgc
    15601 ctgcttatcg ccctgcttca gcaactcaat cgcctcgccg aaacgcttat agtaacgttt
    15661 gattaacgcc agattacgct ctgacgacat aataatgtcg gcataaagct gcggatcctg
    15721 agcaaacagt cgcccgacca tcgccagctc aaggcggtaa atcggcgaag agagcgccag
    15781 aagttgctca agctgaacat tttcttctgc cagatgcagc ccataagcaa aagtagcaaa
    15841 gtggcgcaga gcctgaataa acgccatatt ctgatcgtgc tcgacagcgc taatacgatg
    15901 cagccgagcg ccccagacct gaatttgctc cagaaaccat tggtatgctt ccggcttacg
    15961 tccatcacac cagaccacaa cttgctttgc caggctaccg ctgtccgggc cgaacatcgg
    16021 gtgtaacccc agtaccgggc catcgtgcgc cgccagcatg gcctgtaatg gtccattttt
    16081 cactgatgcc agatcaacca gaatacaatc tttcggtaaa ggcggtaatt tgccgataac
    16141 ttgctcagta acgtggattg gcacactaac aatcaccatt ccggcatcgg caacaatatc
    16201 agccgctcga tcccagtcat gttgctccag aatccgcacc tgataacccg agagtgtcag
    16261 catcttctcg aacaggcgtc ccatctgacc gccaccgccg acgataacca ccggacgcag
    16321 tgacggacaa agtgttttaa atcctttgtc gttttcactg gagtaagatt cacgcatcac
    16381 ccgacgcaaa acatcctcaa tcagatctgg cggtaccccc agagcttccg cctctgcacg
    16441 acgcgaggcc aacatagatg cctcgcgctc cggaacataa ataggcagtc caaagcggct
    16501 tttcacctcg cccacttcag caaccagttc cagacgcttc gctaataaat tcagcagcgc
    16561 tttatcgact tcatcaattt gatcgcgtaa tgcggtcaat tcagcaacca taataaacct
    16621 cttaagccac gcgagccgtc agctgcccgt tcagatcctg atgaatttca cgcagcaagg
    16681 catcggtcat ttcccagcta atgcaggcat cggttacgga tacaccgtat ttcatttcac
    16741 tgcgcggttg ctcagaagac tgattgccct cgtggatatt actttcgatc atcagaccaa
    16801 taattgagcg attgccatct ttgatttgag caaccacgga ttctgccacc gcaggctgac
    16861 ggcgataatc tttattggaa ttaccgtggc tgcaatctac catcagagac gggcgcagtc
    16921 ccgcctgttc catctctttt tcacattgcc caacatccgc agggctatag ttcggcgctt
    16981 taccaccgcg caggatgaca tgcccgtccg gattcccctg agtttgtagc aacgcaacct
    17041 gccctgcctg gttaatgcca acaaaacggt gcggctgggc ggcggcgcgc atagcgttaa
    17101 ttgctgttgc cagactgccg tcggtgccgt ttttaaaacc aaccggcatg gaaagcccgg
    17161 aggccatttc acggtgagtt tgcgattccg ttgtacgagc accaattgct gaccagctaa
    17221 acagatcgcc caggtattgc gggctattcg gatctaacgc ttccgtcgcc agtggcagtc
    17281 ccatattcac cagctcaagc agcaatttac gcgcgatctg cagcccggct tctacatcaa
    17341 aagagccatc catatgggga tcgttaatta accctttcca gccgacagtg gtacggggtt
    17401 tttcaaaata gacgcgcatt accagataga ggctatcgct gacctctgcg gcaagggctt
    17461 taaatcgacg agcatattcc agagcagttt ccagatcatg aatggaacaa ggaccacaaa
    17521 ctaccagcag acgaggatcg cgcccggcga taatatctga aatggtttta cgcgagtcag
    17581 caatctgggc ttcttgttgc aggctcaatg gaaaagcggc cttcagttgt tccggagtca
    17641 ttaaaacctg ttcgtcggta atatgtacgt tattcagcgc gtctttttgc atgatggcga
    17701 tcctgtttat gctcgtttgc gatagttgat cctcagcgag gatgacgtaa cgataacaca
    17761 taaagtaaag ttttcaatcc atatttcgta cgcttttctt tacacgaaca atttactcgc
    17821 gctttgatgc ttatttcgta caaataaatt tacaccccct ttaaaaacaa tccgctatgc
    17881 tttgaaaaag gagaaagaaa tgatgaaaaa gtttatcgcc cccttattgg ctttactggt
    17941 tagcggatgt cagattgatc cttatactca cgcgccaacc ttgaccagca ccgactggta
    18001 tgatgtcggt atggaagatg cgatatcggg cagcgccata aaagatgacg atgcatttgg
    18061 cgattcacag gcggatcgcg gtctatacct taaaggatat gccgaaggac aaaagaaaac
    18121 ttgccagacc gattttactt atgcccgggg actttccggt aaaagctttc ctgccagctg
    18181 taacaatgtt gaaagtgcca gccaactgca tgaagtatgg caaaaagggg ctgatgaaaa
    18241 tgccagcaca atacgattaa attaattaaa accccacaaa taaattaaac ataagatttt
    18301 actcattgtg ttttatttct cacattgatg acgatcgcat gaaaaaatga tattttaatg
    18361 agcattctga aaaaatatcg atgcatttcg agcgaagaag gtggggatcc ctgaatgcgt
    18421 ttttctcacc gactgttctt acttctgatt ctcctgctga cgggagcacc aatactcgct
    18481 caggagcctt ctgacgttgc gaaaaacgtg cgtatgatgg tttccggtat tgtcagttac
    18541 acccgctggc ctgcgctatc agggccacca aagttatgta tcttttcatc ttcacgtttt
    18601 agtaccgccc tacaagagaa tgccgcgaca tctttgcctt atctgcctgt catcattcac
    18661 actcaacaag aggcgatgat ttcaggctgc aatggttttt attttggcaa cgagtcaccg
    18721 acattccaga tggaattaac cgaacaatat ccgtcaaagg cgttattatt aattgccaaa
    18781 caaaataccg agtgcattat tggtagcgcc ttttgtctga tcatccacaa taatgacgtc
    18841 agatttgccg taaacctgga tgccttatcg cgtagcggtg taaaggtcaa cccggatgtc
    18901 ttaatgctcg cacggaagaa aaatgatgga taacgataat tctcttaata agcgccccac
    18961 gtttaaaaga gcattacgca acatcagtat gaccagcata tttatcacta tgatgctgat
    19021 ctggttgctg ctttccgtga cctcggtgct gaccctgaaa cagtacgcgc aaaaaaacct
    19081 ggcactgaca gcagcaacaa tgacttacag tctggaagca gctgtcgttt ttgccgatgg
    19141 ccctgcagca actgaaacac tggcagcgct gggccagcaa gggcaatttt caactgcaga
    19201 agtacgtgat aagcagcaaa atattctggc gtcctggcat tacacccgta aggatccagg
    19261 cgatactttc agcaatttca taagccactg gctcttcccc gcccccatca ttcagccgat
    19321 tcgtcacaat ggtgaaacca ttggcgaagt acgcttaacc gctcgcgaca gttcaatcag
    19381 ccattttatc tggttttcgc tcgccgtact gaccggttgt attctgctgg catcaggcat
    19441 cgcaattacc ctcacccgcc atttgcacaa tggcctagtg gaagcgctga aaaatatcac
    19501 cgatgtcgta catgatgtgc gttccaaccg caatttttcc cgacgagttt ctgaagaacg
    19561 tatcgctgag tttcaccgct tcgctctcga cttcaacagt ctgctggatg aaatggaaga
    19621 gtggcagctt cgtttacagg ccaaaaatgc gcagcttctg cgtaccgcgc tacatgaccc
    19681 attaaccggg ctggctaacc gcgcagcgtt tcgtagcggc atcaatac
//
LOCUS       DAZPEB010000061        19438 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZPEB010000061 DAZPEB010000000
VERSION     DAZPEB010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19438
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.062"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..732
                     /gene="rihB"
                     /locus_tag="ACHURJ_001911"
     CDS             <1..732
                     /gene="rihB"
                     /locus_tag="ACHURJ_001911"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFR1280878.1"
                     /translation="IMRKQIVADNIHGETGLDGPVFESLTRQAESTHAVKYIIDTLMA
                     SDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFTPSAEFNIFADPE
                     AARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSDIMNFTLKTQFEN
                     YGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCDELGVLGKPANTK
                     VGITIDTDWFWGLVEECVRGYIKTH"
     gene            832..2082
                     /locus_tag="ACHURJ_001912"
     CDS             832..2082
                     /locus_tag="ACHURJ_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFR1280879.1"
                     /translation="MDVMRSVLGMVVLLTIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPAGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDHLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFAHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRVPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(2138..3226)
                     /locus_tag="ACHURJ_001913"
     CDS             complement(2138..3226)
                     /locus_tag="ACHURJ_001913"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFR1280880.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGIADIRYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTAPAHTTVDSFGAGDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(3229..4086)
                     /gene="nfo"
                     /locus_tag="ACHURJ_001914"
     CDS             complement(3229..4086)
                     /gene="nfo"
                     /locus_tag="ACHURJ_001914"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFR1280881.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(4160..5209)
                     /locus_tag="ACHURJ_001915"
     CDS             complement(4160..5209)
                     /locus_tag="ACHURJ_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFR1280882.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            5309..6190
                     /gene="yieE"
                     /locus_tag="ACHURJ_001916"
     CDS             5309..6190
                     /gene="yieE"
                     /locus_tag="ACHURJ_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFR1280883.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANIPR"
     gene            6395..7864
                     /gene="lysP"
                     /locus_tag="ACHURJ_001917"
     CDS             6395..7864
                     /gene="lysP"
                     /locus_tag="ACHURJ_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFR1280884.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            8157..10137
                     /gene="cirA"
                     /locus_tag="ACHURJ_001918"
                     /pseudo
     CDS             8157..10137
                     /gene="cirA"
                     /locus_tag="ACHURJ_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(10169..11005)
                     /gene="yeiG"
                     /locus_tag="ACHURJ_001919"
     CDS             complement(10169..11005)
                     /gene="yeiG"
                     /locus_tag="ACHURJ_001919"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFR1280885.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSSYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            11263..11931
                     /gene="folE"
                     /locus_tag="ACHURJ_001920"
     CDS             11263..11931
                     /gene="folE"
                     /locus_tag="ACHURJ_001920"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFR1280886.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            11948..13105
                     /gene="yeiB"
                     /locus_tag="ACHURJ_001921"
     CDS             11948..13105
                     /gene="yeiB"
                     /locus_tag="ACHURJ_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFR1280887.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNRAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            13247..14287
                     /gene="galS"
                     /locus_tag="ACHURJ_001922"
     CDS             13247..14287
                     /gene="galS"
                     /locus_tag="ACHURJ_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFR1280888.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAIFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            14567..15565
                     /gene="mglB"
                     /locus_tag="ACHURJ_001923"
     CDS             14567..15565
                     /gene="mglB"
                     /locus_tag="ACHURJ_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFR1280889.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQSVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            15626..17146
                     /gene="mglA"
                     /locus_tag="ACHURJ_001924"
     CDS             15626..17146
                     /gene="mglA"
                     /locus_tag="ACHURJ_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFR1280890.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLRRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDKSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            17162..18172
                     /gene="mglC"
                     /locus_tag="ACHURJ_001925"
     CDS             17162..18172
                     /gene="mglC"
                     /locus_tag="ACHURJ_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFR1280891.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPCWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(18415..19316)
                     /gene="preA"
                     /locus_tag="ACHURJ_001926"
                     /pseudo
     CDS             complement(18415..19316)
                     /gene="preA"
                     /locus_tag="ACHURJ_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
BASE COUNT         4632 a         4809 c         5145 g         4852 t
ORIGIN      
        1 attatgcgta aacaaatcgt tgccgataat attcacggtg aaaccggact ggatggtccg
       61 gtattcgagt cgctgacccg ccaggcagaa agtacccacg cggtgaaata catcattgat
      121 accctgatgg caagcgatgg cgatatcact ctggtgccgg ttggtccgct ttcaaatatc
      181 gcggtggcaa tgcgtatgca acccgcgatc ctgcccaaaa tccgtgaaat tgtgctaatg
      241 ggcggcgctt acggtacagg caacttcacg ccatctgccg agttcaacat ctttgctgat
      301 ccggaagcag cccgcgtggt gttcacctcc ggcgttccat tagtgatgat gggcctcgat
      361 ctcaccaacc agactgtttg caccccggac gtgattgcac ggatggaaag ggcaggcggc
      421 cctgccggag agctgttcag cgacatcatg aacttcactc tcaaaacgca gttcgaaaac
      481 tacggcctgg ctggcggccc ggtgcacgac gccacctgca tcggttatct gattaaccct
      541 gatggcatta aaacccagga gatgtacgtc gaagtggacg tcaacagtgg cccttgctat
      601 gggcgtaccg tctgcgacga gctgggcgtt cttggcaagc ccgctaatac caaagtcggc
      661 atcactattg ataccgactg gttctggggc ttagtcgaag agtgcgtgcg cggctacatc
      721 aaaacccatt aagtgttgat aacaagccgg gtcagtatcg caagatccaa gcccggctat
      781 ttctgatggg cagaaagccg tcgcccaata tgacatgaag agataataac tatggatgtc
      841 atgagaagtg ttctgggaat ggtggtattg ctgacgattg cgtttttgct gtcagtaaac
      901 aagaagaaga tcagcctgcg taccgttggc gcggcgttag tgttacaggt cgtgattggc
      961 gggattatgc tttggttacc ggcagggcgt tgggtcgctg aaaaagtcgc ttttggcgtg
     1021 cataaagtga tggcgtacag cgacgcgggt agcgcattta tcttcggttc gctggtcggg
     1081 ccgaaaatgg acacgctgtt tgatggcgca ggatttatct ttggtttcag ggtattaccg
     1141 gcaattatct tcgtcactgc actggtgagt attctctact acatcggtgt gatggggatt
     1201 ttaattcgca ttctcggcgg tatattccag aaagcattaa atatcagcaa gattgagtca
     1261 ttcgtcgcgg tcaccaccat tttcctcggg caaaacgaaa ttccggcgat cgtcaaaccc
     1321 tttatcgatc atctgaatcg caatgaatta tttacagcga tttgtagtgg catggcctcg
     1381 attgctggtt cgacaatgat tggttacgcc gccctgggcg tacctgtgga atatttgctg
     1441 gcggcatcgt taatggcgat cccaggcggg atcttgtttg ctcgcctgtt aagcccggct
     1501 acggaatctt cgcaggtttc ctttaataac ctctctttca ccgaaacacc gccaaaaagc
     1561 attattgaag ccgctgcgac aggggcaatg accgggctga aaatcgccgc cggtgtagcg
     1621 acagttgtta tggcatttgt cgccatcatt gcgttaatta atggtattat cggcggcgtt
     1681 ggcggctggt ttggttttgc acatgcctcg ctggagtcca ttttaggtta cctgttggcc
     1741 ccattggcgt gggtgatggg ggttgactgg agtgatgcaa atcttgccgg gagtttgatt
     1801 gggcagaagc tggcgatcaa tgaatttgtc gcttatctca atttctcgcc atatctgcaa
     1861 acgggtggca ctctggatgc taaaaccgtg gcgattattt ctttcgcgtt gtgcggtttc
     1921 gctaactttg gttctatcgg ggtggtggtg ggggcgtttt ctgcggttgc gccacaccgt
     1981 gtgccggaaa tcgcccaact tggtttacgc gcgctggcgg cggcgacact ttctaacctg
     2041 atgagtgcta ctattgcagg attctttatt ggtttagcgt agtgcgcaat ttatgagccg
     2101 gataagatgc ggtcaagcgt cgaatccggc aacattgtta caccattggc actaatgaaa
     2161 gcgcgttatc ggcagacagg gtagggttgt ttaagctgcc gctggcgcgc gaaattgccg
     2221 cgcaggctac cgcaaaacgg gcgctgtcgc ggaaactgta tccttcaaga aagctatata
     2281 ccaggcccgc cataaaacca tcgccagcac caaaactgtc tactgtcgta tgcgctggcg
     2341 cggtcagcaa aaattgttct ccatcctttt cgctgcaata aactgactca tcgggcaaat
     2401 aaacaaacag ttgctgaaca ccttgctgat gcaatgcatt cactgcggca ttacggtcag
     2461 catcgctggt gatcgcctgt ccccataaaa tttccagctc cggtaaagtg ggtttcaggg
     2521 tgtgaatatg cgccagccag tgtttgattt tgcccgcttt gaattctgaa acggtatcga
     2581 caaacaccgg gatttcatca gcgagggtaa agacccattc cagcgcctcg gctgtcaggt
     2641 tgcaatcagc cagtaccacg cccgcatgac gaagtaaatc gcgcgacccg ttcagtagtt
     2701 gcggtgtcaa ctgttccagc agatgggtat cgttaatcgc cagcacggtt tgatcgtctc
     2761 gattggcaat tgccagatac gtcgatgtgc tttgaccatg caaacgaacg cagccggaga
     2821 cattcacgcc ggcacggcgc gtttcttcca ggagcatttc gccataaaag tcatcgccaa
     2881 tcactgaaag caaatggacg tcacggccta acagcgccag attgtgggcg atgttgcgtc
     2941 ccacgccgcc cgccgagcaa tgaattgtac cgggatgaga agccgcttgc gggtaacgga
     3001 tatccgcgat cccgcgaata tccatattga ttgtccccac cactacgcag tattcctgct
     3061 cggtgagaat gtaacctttg cctttaatcc gccctttgcg cattaaatcc ataatatgcg
     3121 ccgcaacgcg cgaacgactg atttgcagca tgtccgcaat ttcgttctgc tgaatcagcg
     3181 ggttacgccg taaaattgca aggatctcct tttcccggtt attcatcttc aggctaccgc
     3241 tttttcagtt tgttgcgctt tcagccaggc gatctcttct gcccagatat ccgggttgat
     3301 ggtttcaagg atcagcggaa tgccgtcgaa acggtcgtcc tgcatgatcc agcggaacgc
     3361 atcatgaccg atattgcctt caccgaggct atgatggcgg tcaacgcggc tgccaaaggt
     3421 gcttttcgca tcgttaaggt gcatcccgcg cagatactta aagccaacaa tacgggcaaa
     3481 atccgcgaac gttttctcgc attcggctgg agtacgcaaa tcatacccgg cagcgaaagc
     3541 atggcaggta tcaatgcaga cgccgacacg ggatttatct tccacgccgt cgataatcgc
     3601 cgcgagatgt tcgaatttaa accctaagtt actgccctga ccggcggtgt tttcaatcac
     3661 cgctgttaca ccttgagttt tatccagcgc aatgttgatg gattcggcaa tacgcgcaag
     3721 gcaatcctct tctgaaatct gcatcagatg gctgccaggg tggaagttga gcaaagaaag
     3781 ccccagttgt tcgcaacgct gcatttcatc tataaaggca tcgcgcgatt tttccagagc
     3841 ttcagtgacc ggatgtccga ggttaatcag atagctgtcg tggggaagaa tttgcgccga
     3901 tgtgtagtga tatttttcac aggctgcttt gaattcatcg atggtttgcg tcgtgagcgg
     3961 tgcggcacgc cactgacgtt ggtttttggt gaacaaggca aacgcggttg cgtcgatttc
     4021 ggcggcgcga attgcggcat ttgccagacc gccagcagca ctaacgtgcg ctccaatgta
     4081 tttcatgcga ggactcctgt taaacccgct ggaggaaaac ggtaatgata gcgggttaac
     4141 aggagcaaga tgtagtggtt tatgcgatga cgctttgaat cacatagtta atcgcaccac
     4201 caccaacaat cagccaggca aacagtacca gtgccatcag cagaggtttc gccccggctt
     4261 ttttcagcgc gctgacgtga gtggtcagac ccagcgccgc catcgccatt gccagcagga
     4321 aggtatccag cgttaccagc atgttcacca cgctctgcgg taacaggtgg aacgagttaa
     4381 agatggcaac gacgatgaac aagatggcaa accacggaat agtgattttg cttttctcgc
     4441 cgctgttcgc cccagacagc tgtttaacac gcgccgccag caggatgagg aacggagcca
     4501 gcatcatcac gcgcagcatt ttggaaataa ctgctgcgtt ttccgcatcc gggctgatgg
     4561 catgacctgc cgccaccacc tgcgccactt cgtgcacagt agaaccaatg tagataccga
     4621 aagtttccgg actaaaccat tgagacatca gcggatatat cgccgggtag aggaaaatcg
     4681 cgacggtccc gaagataaca acggttgcaa cagccacggt tactttactg gcttccgctt
     4741 tcactaccgg ctcagtcgcc agtaccgcgg cagcaccaca gatactgcta ccggcaccga
     4801 tcaaccagct ggtgtgctta tccagaccaa acactttctg ccccaggaag caagccagca
     4861 ggaaggtact ggacagcgtc aacacgtcaa tgatgatccc actgataccg acatcggcaa
     4921 tttgcgagaa cgtcagacgg aagccataaa gaatgatacc cagacgtaat aaatattgct
     4981 tggcaaacag cacaccaccg tcacagcttt tccagatgtg cggatagatg gtgttgccta
     5041 aaaccatccc caacaagatt gcgagggtga gggcactaaa cccggcaccc gcaaccgcgg
     5101 gaatggaacc accccacagg gcgaccccgg tgataactgc actcagggct aaccccggaa
     5161 taaaatgcca cagtgtacga tgttgtttct gtaaggtgat attcgtcata accctctcct
     5221 tataaccggg cataaggtta cgactaactg gtttaaaaat aaaattgatt atatatttat
     5281 aattaatctt tataagtggt aagcgactat gcacatcacc ctccggcagt tggaagtttt
     5341 tgcagaagta ttgaaaagtg gatcaaccac ccaggcgtcg gtgatgctgg cgttgtcgca
     5401 atcagcagtg agcgcagcct tgaccgacct ggaagggcag cttggcgtgc aactgtttga
     5461 tcgcgtgggg aaaagactgg ttgttaatga acacgggcgg ctgctctatc cgcgtgcgtt
     5521 ggcattgctt gaacaggcgg ttgaaatcga acaactgttt cgcgaagaca acggcgcgat
     5581 tcgtatctat gccagtagta ccatcggtaa ctacattctg cctgcagtta tcgcccgtta
     5641 tcgccatgat tatccgcagt tgccgattga acttagcgtt gggaacagcc aggacgtgat
     5701 gcaagcggtg ctggatttcc gcgttgatat tggctttatt gaaggaccgt gccacagcac
     5761 tgaaatcatt tctgaaccgt ggctggaaga cgagctggtg gttttcgccg cgccgacttc
     5821 gccgttggct cgtggtccgg tcaccttaga acagctggcc gctgcgccgt ggatcctgcg
     5881 tgaacgcggt tccggcacgc gggagattgt cgattatcta ttgctgtcac atttaccgaa
     5941 gtttgagatg gcgatggaat taggtaactc cgaggcaatc aaacatgcgg tgcgtcatgg
     6001 gttgggaatt agttgcctgt cgcgacgggt gattgaagat caattgcagg caggcacatt
     6061 aagtgaagtt gcggtccctc tgccgcgcct gatgcgtacg ttgtggcgta tacatcatcg
     6121 gcaaaaacac ctttccaacg cgctacggcg ctttctggac tattgcgatc ccgcgaatat
     6181 accgcgttaa gttgctgtac aagaacatgc tggtgctgtg tcgatttcgt gacgcagcgc
     6241 cctcagcatg cattcgccag aaaagagatt ggctgcttta cttataatcc cagggcgatc
     6301 atgaaggtgt cttataaccg tgtatttctg ccggaaggat tgccaatcgt ctgctacaat
     6361 cgcgcctcat ttttaagatg gatagcattt ttgtatggtt tccgaaacta aaaccacaga
     6421 agcgccgggc ttacgccgtg aattaaaggc gcgtcacctg acgatgattg ccattggcgg
     6481 ttccatcggt acaggtcttt ttgttgcctc tggcgcaacg atttctcagg caggtccggg
     6541 cggggcattg ctctcgtata tgctgattgg cctgatggtt tacttcctga tgaccagtct
     6601 cggtgaactg gctgcatata tgccggtttc cggttcgttt gccacttacg gtcagaacta
     6661 tgttgaagaa ggctttggct tcgcgctggg ctggaactac tggtacaact gggcggtgac
     6721 tatcgccgtt gacctggttg cagctcagct ggtcatgagc tggtggttcc cggatacacc
     6781 gggctggatc tggagtgcgt tgttcctcgg cgttatcttc ctgctgaact acatctcagt
     6841 tcgtggcttt ggtgaagcgg aatactggtt ctcactgatc aaagtcacga cagttattgt
     6901 ctttatcatc gttggcgtgc tgatgattat cggtatcttc aaaggcgcgc agcctgcggg
     6961 ctggagcaac tggacaatcg gcgaagcgcc gtttgctggt ggttttgcgg cgatgatcgg
     7021 cgtagctatg attgtcggct tctctttcca gggaaccgag ctgatcggta ttgctgcagg
     7081 cgagtccgaa gatccggcga aaaacattcc acgcgcggta cgtcaggtgt tctggcgaat
     7141 cctgttgttc tatgtgttcg cgatcctgat tatcagcctg attattccgt acaccgatcc
     7201 gagcctgctg cgtaacgatg ttaaagacat cagcgttagt ccgttcaccc tggtgttcca
     7261 gcacgcgggt ctgctctctg cggcggcggt gatgaacgca gttattctga cggcggtgct
     7321 gtcagccggt aactccggta tgtatgcgtc tactcgtatg ctgtacaccc tggcgtgtga
     7381 cggtaaagcg ccgcgcattt tcgctaaact gtcgcgtggt ggcgtgccgc gtaatgcgct
     7441 gtatgcgacg acggtgattg ccggtctgtg cttcctgacc tccatgtttg gcaaccagac
     7501 ggtatacctg tggctgctga acacctccgg gatgacgggt tttatcgcct ggctggggat
     7561 tgccattagc cactatcgct tccgtcgcgg ttacgtattg cagggacacg acattaacga
     7621 tctgccgtac cgttcaggtt tcttcccact ggggccgatc ttcgcattca ttctgtgtct
     7681 gattatcact ttgggccaga actacgaagc gttcctgaaa gataccattg actggggcgg
     7741 cgtagcggca acgtatattg gtatcccgct gttcctgatt atttggttcg gctacaagct
     7801 gattaaagga actcacttcg tacgctacag cgaaatgaag ttcccgcaga acgataagaa
     7861 ataagtttcc tcccttcctt gctaagccct ctcaaccgag agggcttttt caattccatt
     7921 tccctgacaa atcatgcgga tataaaattt aacatttgga ttgataattg ttatcgtttg
     7981 cattatcgtt acgccgcaat caaaaaaggc tgacaaatca gaggctgttc cggctttctg
     8041 ggatgatcac ctgcatacaa ataagtccac cgcgatgctg ccgtacgcaa ggggacgtga
     8101 agaaggtgtg agcgataacc cattttattt tcgtagttac ctcatggaga tatggaatgt
     8161 ttaggttgaa ccctttcgta cgggtcaggc tgtgtttgtc cgctatttct tgtgcatggc
     8221 ctgtgttagc ggtcgatgat gatggcgaaa cgatggttgt cactgcatct tccgttgaac
     8281 aaaacctcaa agatgcaccc gccagtatca gcgtcattac ccaggaagac ctgcagcgaa
     8341 aaccggtaca gaatctgaag gatgtcctca aagaagtgcc tggcgtacaa ctgacgaacg
     8401 aaggggataa ccgtaagggc gttagtattc gtggtctgga cagcagctac accctgattc
     8461 tcgtcgacgg taaacgcgtt aactcctgca atgccgtctt ccgccacaat gatttcgatc
     8521 tgaactggat cccggtcgat tccatcgaac gtattgaagt ggttcgtggc ccgatgtcgt
     8581 cgctatacgg ctccgatgcg ctcggcggtg tagtgaatat catcaccaaa aaaatcggtc
     8641 agaaatggtc gggtaccgtt accgtcgata ccaccattca ggaacatcgc gatcgcggtg
     8701 atacctataa cggtcagttc tttaccagtg gaccattaat tgatggtgtg ctgggaatga
     8761 aagcttacgg cagcctggca aaacgtgaaa aggatgaccc gcaaaactca acgaccaccg
     8821 ataccggaga aatgccgcgt attgaaggat tctccagccg cgacggcaat gtcgaatttg
     8881 cctggacacc gaatcaaaat cacgatttta ctgccggata cggtttcgac cgtcaggaac
     8941 tcgctggaca aaaaccgcct ggaacgccag aactactccg tcagccataa tgggcgttgg
     9001 gattacggca ccagcgaact gaaatactac ggtgagaaag tcgagaacaa aaaccctggc
     9061 aacagcagcc cgataacttc cgaaagcaat acggtcgacg gcaaatacac gttgccgctg
     9121 acggcgatta atcagtttct cacggttggc ggtgaatggc gtcacgacaa acttagcgat
     9181 gcggtgaacc tgaccggggg aaccagctcc aaaacgtctg ccagccagta cgcgctgttt
     9241 gtggaagatg aatggcggat cttcgagccg ctggcgctga cgaccggcgt gcgtatggac
     9301 gatcacgaaa cctacggtga acactggagt ccgcgtgcct acctggttta taacgccacc
     9361 gacaccgtaa cggtgaaagg gggctgggcg acggcattta aagcaccttc tctgttgcaa
     9421 cttagccctg actggacgag caattcctgc cgtggcgcat gtaagattgt gggtagcccg
     9481 gatctgaaac cagaaaccag cgaaagttgg gagctggggc tttactacat gggtgaagaa
     9541 ggctggctgg aagggggtga atccagcgtt accgttttcc gtaacgatgt gaaagatcgt
     9601 atcagcatca gccgtacgtc tgacgtcaac gctgcaccgg gctaccaaaa ctttgttggt
     9661 tttgagacgg gcgctaacgg acggcgcata ccggtattta gctactacaa cgttaacaaa
     9721 gctcgtattc agggcgtgga aaccgaactg aaaattccgt tcaacgatga atggaaactg
     9781 tcgatcaact acacctacaa cgatggtcgt gatgtcagca acggcgaaaa caaaccgcta
     9841 tccgatctgc cgttccatac tgctaacggt acgctggact ggaaaccgct ggcgctggaa
     9901 gactggtcat tctatgtttc tggtcactat accgggcaga aacgcgccga cagcgcgacg
     9961 gctaaaacac cgggcggtta taccatctgg aataccggcg cggcctggca ggtgactaaa
    10021 gacgtcaaac tgcgcgcagg cgtgctgaac cttggcgaca aggatctcag tcgtgacgac
    10081 tacagctata acgaagacgg acgtcgttac tttatggcag tggattatcg cttctgatga
    10141 gaagatgccc ggcgaaccgg gcggactttc acttcagtaa atactgcgca tggaagcgca
    10201 ggtgatcctc tataaaagag gcgatgaagt agtaactgtg atcatatccc ggctgaatac
    10261 gcagcgtcat cggccacgct ttctggcgcg cggcttctgc cagtaccgca ggttgcaact
    10321 ggtcggcaag aaactgatca ttatcgccct gatcgataag cgtcgggatc gcatcctgcg
    10381 cgttactggc atacatcagc gcgcaactgt cccattccag ccatgcattt ttgtcctcac
    10441 ctaaatagct gctaaacgct ttgattcccc acgggacgct gcacggattc acaattggcg
    10501 caaaggccga aacgctggtg tatttacccg gatttttcag cgccataatc agcgcaccgt
    10561 gaccacccat tgagtgaccg ctaatggcgc agcggtcgct gacattaaat tgcgactgaa
    10621 ccagcgccgg taattcatcg cgcagataat catacatccg gtaatgcgtc gcccacggcg
    10681 gttgcgtggc attaagataa aagcctgcgc cctggcctaa atcgtagcca tcgtcgttgg
    10741 caaccttttc gccgcgcggg ctggtgtctg gcatcaccag tacaatcccc agttctgccg
    10801 ccacgcgctg ggcacccgcc ttggtggtga agttctcgtc attgcaggtt aatccggaaa
    10861 gccagtacag cactggtggc ggggtgtgat cacgaggggg tgggagaaag atactgaacg
    10921 tcatcgggca gtttaaggta ctggagtcgt gtcgccagcg ttgctgccag ccttcaaaac
    10981 agcggtgctc ttcgagcatt tccatgcgtg gctccttgtt gtgttgtttg caatttcttc
    11041 atcataccgt ttattcaatg tgctgtgagt aactttcact tccgtatttg cataacgatg
    11101 ttttaacatc tgctgatgaa aggcagcggc aattacaata attatcgctg tgaatactgg
    11161 attatgtgcg ccgcctcacg cacaataatc aggctgtaaa tcagcttaat aactttgccc
    11221 ccacgcaggg cggaggcgtc acacctgcag gagaaatcat aaatgccatc actcagtaaa
    11281 gaagcggccc tggttcatga agcgttagtt gcgcgaggac tggaaacacc gctgcgcccg
    11341 cccgtgcatg aaatggataa cgaaacgcgc aaaagcctta ttgctggtca tatgaccgaa
    11401 atcatgcagc tgctgaatct cgacctggct gatgacagtt tgatggaaac gccgcatcgc
    11461 atcgctaaaa tgtatgtcga tgaaattttc tccggtctgg attacgccaa cttcccgaaa
    11521 atcaccctca ttgaaaacaa aatgaaggtc gatgaaatgg tcaccgtgcg cgatatcact
    11581 ctgaccagta cctgtgaaca ccattttgtt accatcgatg gcaaagcgac ggtggcttat
    11641 atcccgaaag attcggtgat cggtctgtca aaaattaacc gtatcgtgca gttctttgcc
    11701 cagcgtccgc aggtgcagga acgtctgacg cagcaaattc ttattgcgct acaaacgctg
    11761 ctgggcacca ataacgtggc tgtctccatt gatgcggtgc attactgcgt gaaagcgcgt
    11821 ggcatccgcg atgcaaccag tgccacaaca acgacctctc ttggtggatt gttcaaatcc
    11881 agtcagaata cgcgccacga gtttctgcgc gctgtgcgtc atcacaactg attaaaaggc
    11941 aggaaccatg gagcgcaacg taacgctcga ttttgttcgc ggcgtcgcca ttctggggat
    12001 cctgctatta aacatcagcg cctttgggct accaaaggcg gcttatctca atcgcgcctg
    12061 gtacggcgct attacgccgc aggatgcatg gacctgggca tttctcgatc tcatcggcca
    12121 ggtgaaattc ctcacgcttt ttgcgctgct gtttggtgcg ggcctgcaaa tgttgctgcc
    12181 ccgtggcaga cgctggatcc agtcgcggtt aacgctgtta gtcttgctgg gttttattca
    12241 cggtttattg ttctgggacg gcgatattct gctggcttac gggctggtgg gcttaatctg
    12301 ctggcggctg gtgcgcgatg cgccatcggt aaaaagcctg tttaatacag gcgtcatgct
    12361 ttatctggtg gggcttggcg ttttgctgtt attggggctg atttccgaca gccagaccag
    12421 ccgcgcctgg acgccggatg catcggctat tttgtatgaa aaatactgga agcttcacgg
    12481 cggcgttgaa gcgatcagta atcgtgccga tggtgttggc aacagtttac tggcactggg
    12541 cgcacagtat ggctggcaac tggcagggat gatgctcatt ggtgcggcgc tgatgcgcag
    12601 cggctggctg aaagggcagt tcagcttacg tcactatcgt cgtactggtt ttgtgctggt
    12661 ggcgattggg gtgaccatta accttcctgc catcgccctg caatggcagc tggactgggc
    12721 gtatcgctgg tgtgccttct tacttcaaat gccgcgggaa ctgagtgcgc cgtttcaggc
    12781 gattggctat gcgtcgctgt tttatggctt ctggccgcaa ttgagccgct ttaagctggt
    12841 gcttgcgatc gcctgcgtcg gacggatggc gctgaccaac tatctattgc aaacgctgat
    12901 ttgtaccatg cttttttacc acctcggttt gtttatgcat tttgaccgcc tggagctgct
    12961 ggcgtttgtt attccggtat ggctggcgaa tatcctcttc tctgttatct ggctgcgtta
    13021 cttccgccag gggccggtgg aatggctctg gcgtcagtta actttgcgtg ctgccggaac
    13081 ggcaatatct aaaacatcaa gataacgatc tggatcacat tcgttaacaa aacggctgta
    13141 accgtttcca ttgctgtgac tcgattcacg aagtcctgta ttcagtgctg acaaaatagc
    13201 cgccagcaag cagtcattta ctgcaatctc ataacaggta gtgaatatga tcaccattcg
    13261 tgatgtagcg cgtcaggctg gcgtctctgt ggcaacggtt tcccgggtgc tcaataacag
    13321 cacgctggtc agtgccgaca cgcgtgaagc agtaatgaaa gccgtgagtg agctggatta
    13381 tcggccaaac gccaatgccc aggcgctggc aactcaggtt agcgacacca ttggcgtggt
    13441 ggtgatggac gtttctgatg cgtttttcgg cgcgctggta aaagcggtgg atctagtcgc
    13501 tcagcagcat cagaaatacg tgctaatcgg caatagctat catgaagcgg aaaaagagcg
    13561 tcacgccatt gaggtgttaa ttcgccagcg ttgtaatgcg ttgattgttc actcaaaagc
    13621 attgagtgac gatgaactgg cgcaatttat ggataacatt cccggtatgg tgttaatcaa
    13681 ccgcgttgtg ccggggtacg cccatcgttg cgtttgcctg gataatctca gcggtgcccg
    13741 aatggcgacg cgcatgttgc tgaataacgg tcatcaacgt attggttatc tttcttccag
    13801 tcacggcatt gaagatgacg ccatgcgtaa agcaggctgg atgagtgcgt tgaaagagca
    13861 ggatattatt ccgccggaaa gctggattgg cactggtacg ccggacatgc cgggcggtga
    13921 ggcggcgatg gttgaactgc tggggcgcaa tctacaactt accgctatat ttgcttataa
    13981 cgacaatatg gccgctggcg cactgacagc attaaaagat aatggcattg cgattccgtt
    14041 acatctctca atcatcggtt tcgatgatat tcccatcgcc cgttacaccg acccgcaatt
    14101 aacgaccgtg cgttatccca ttgcttcaat ggctaaatta gccaccgaac tggccttgca
    14161 gggggcagca ggcaatattg atcctcgtgc cagccactgt tttatgccga cgttagtgcg
    14221 tcgccattct gtcgcaacgc gccagaatgc ggcggcgatc actaactcaa caaatcaggc
    14281 gatgtaaccg ctttcaatct gtgagtgatt tcacagtatc ttaacaatgt gatagctatg
    14341 attgcaccgt tttaacgttg taacccgtat gtaacagtga ataatcactt tcaccgaggt
    14401 aatagcgtca taacaacaat taaagccgtt ttctggagcg ttaccgggca tggaagaacg
    14461 aattttaaaa agtgagcttc ggcgttcagt aacacttcat taactctact gccctgccga
    14521 gcatttatct caagcactac cctgcataag aaaaaccgga gataccatga ataagaaggt
    14581 gttaaccctg tctgctgtga tggccagcat gttattcggt gccgctgcac acgctgctga
    14641 tactcgcatt ggtgtaacaa tctataagta cgacgataac tttatgtctg tagtgcgcaa
    14701 ggctattgag caagatgcga aagccgcgcc agatgttcag ctgctgatga atgattctca
    14761 gaatgaccag tccaagcaga acgatcagat cgacgtattg ctggcgaaag gggtgaaggc
    14821 actggcaatc aacctggttg acccggcagc tgcgggtacg gtgattgaga aagcgcgtgg
    14881 gcaaaacgtg ccggtggttt tcttcaacaa agaaccgtct cgtaaggcgc tggatagcta
    14941 cgacaaagcc tactacgttg gcactgactc caaagagtcc ggcattattc agggcgattt
    15001 gattgctaaa cactgggcgg cgaatcaggg ttgggatctg aacaaagacg gtcagattca
    15061 gtccgtactg ctgaaaggtg aaccgggcca tccggatgca gaagcacgta ccacttacgt
    15121 gattaaagaa ttgaacgata aaggcatcaa aactgaacag ttacagttag ataccgcaat
    15181 gtgggacacc gctcaggcga aagataagat ggacgcctgg ctgtctggcc cgaacgccaa
    15241 caaaatcgaa gtggttatcg ccaacaacga tgcgatggca atgggcgcgg tagaagcact
    15301 gaaagcacac aacaagtcca gcattccggt gtttggcgtc gatgcgctgc cagaagcgct
    15361 ggcgctggtg aaatccggtg cactggcggg cacagtactg aacgatgcta acaaccaggc
    15421 gaaagcgacc tttgatctgg cgaaaaacct ggccgatggc aaaggtgcgg ctgatggcac
    15481 caactggaaa atcgacaaca aagtggtccg cgtaccttat gttggcgtag ataaagacaa
    15541 cctggctgaa ttcagcaaga aataagactg attgggcgca gtcgtttact gcgcccttat
    15601 tgtatggcca acaaggtaaa attatatggt cagctcaacg actccgtctt ccggggaata
    15661 cttgttggaa atgagcggta tcaacaagtc ctttcctggt gttaaggcac ttgataacgt
    15721 taatttaaaa gtccggccac attctatcca tgcattaatg ggggaaaacg gtgcaggaaa
    15781 atcgacatta ttaaaatgcc tgtttggtat ttatcaaaaa gactccggca ccattttatt
    15841 ccagggtaaa gagatcgatt tccattccgc caaagaggca ctggaaaatg gtatttcgat
    15901 ggtacaccag gagttaaacc tggtattaca acgttcggtg atggataaca tgtggctgag
    15961 gcgatatccc accaaaggca tgtttgtcga tcaggacaaa atgtaccgcg aaaccaaagc
    16021 gatttttgat gaactggata ttgatatcga tccgcgtgcg cgcgtcggca cattatccgt
    16081 ttcgcaaatg cagatgatcg aaatcgccaa agcgttttcc tataacgcga aaattgtgat
    16141 tatggatgaa ccgacttctt cgttaaccga aaaagaggtc aatcatctgt tcactattat
    16201 tcgtaaatta aaagagcgcg gctgcggtat tgtttatatc tcgcataaaa tggaagaaat
    16261 cttccagtta tgtgatgaag ttaccgtatt gcgcgacggt cagtggatcg ccaccgaacc
    16321 gctggcagga ctgacgatgg acaagatcat cgccatgatg gttgggcgtt ctcttaatca
    16381 gcgttttcct gacaaagaaa acaagccggg cgaagtcatc ctcgaggtgc gtaacctaac
    16441 gtcactgcgc cagccgtcga ttcgcgatgt ctcgtttgat ctgcataaag gggagatcct
    16501 cggtattgcc gggctggtgg gggcgaaacg taccgatatt gttgagacgt tatttggtat
    16561 tcgcgagaaa tcggctggca ccattacgtt gcacggcaaa cagatcaata accataatgc
    16621 caacgaagcc ataaaccacg gatttgcact ggtaactgag gagcgccgct caacgggaat
    16681 ttatgcctat ctggatattg gttttaactc gttaatttcc aatattcgca actacaaaaa
    16741 taaagttggt ttactggata aatcgcggat gaaaagcgat acccagtggg tgattgattc
    16801 gatgcgggta aaaacaccgg ggcatcggac gcaaattggt tcgctctcag gtggtaatca
    16861 gcaaaaggtg attattgggc gctggctact aacgcaacca gaaatattaa tgctcgatga
    16921 accgacgcgc ggtattgatg tcggggcgaa gtttgagatc tatcagttaa ttgccgaact
    16981 ggcgaagaaa ggcaaaggga ttattattat ctcctctgaa atgcctgagt tgttagggat
    17041 aacagaccgt attctggtca tgagcaatgg tctcgtttcc ggaattgtcg acacaaaaac
    17101 aacaacgcaa aacgaaattc tgcgtcttgc gtctttgcac ctttaagatt aggggcttcc
    17161 catgagtgcg ttaaataaga aaagttttct tacttacctg aaagagggcg gtatttacgt
    17221 cgttctttta gttttgctgg cgattattat tttccaggac ccaacatttt taagtctgtt
    17281 gaacttaagt aatattctca cccagtcatc ggtgcgtatt attatcgcgc tcggtgtggc
    17341 agggttaatt gtcacccagg ggaccgacct ttctgcgggg cgtcaagtag ggctggcagc
    17401 agtggtggca gcgacattat tgcagtccat ggataacgcc aataaagtgt tcccggaaat
    17461 ggcgacgatg ccgattgcgc tggttattct gattgtctgt gccattggtg cggtgatcgg
    17521 tttgatcaac ggtctgatta tcgcttatct caacgttacg ccgttcatta ccacgctcgg
    17581 cacgatgatc atcgtctatg gcatcaactc gctctattac gactttgtcg gggcgtcgcc
    17641 aatttctggt tttgacagtg gcttctctac ctttgctcag ggctttgtcg cgctggggag
    17701 tttccgtctc tcttacatca ccttctacgc gttgattgcg gtggcgttcg tctgggtgtt
    17761 gtggaacaaa acccgcttcg gtaagaacat ttttgccatt ggcggtaacc cggaagcggc
    17821 aaaagtatct ggtgtcaacg tcggcctgaa cctgctgatg atctacgcgt tgtctggcgt
    17881 gttctatgcc tttggcggga tgttagaagc cggacgtatc ggctctgcca ccaacaacct
    17941 cggctttatg tatgagctgg atgctatcgc ggcgtgcgtg gtaggcggtg tatcgttcag
    18001 cggcggtgtg gggacggtga ttggcgtggt gaccggggta attattttta ccgtcatcaa
    18061 ctatggcctg acgtatatcg gcgtaaaccc atgctggcag tacatcatca aaggggcgat
    18121 tattatcttc gccgtagcgc tggattcact gaaatacgcg cgtaagaaat gatgtgaatg
    18181 tggtccgcat tcggtcataa acgcacataa catcatgatc tgtgtggttt tgtaggccgg
    18241 ataaggcgtt cacgccgcat ccggcaacag gagccaaatg ccggatgcaa cgctggcgcg
    18301 ttttatccgg cctacaataa gctcaacata ctgatttgta tggttttgta ggccggataa
    18361 ggcgttcaag ccgcatccgg cgacaggaac caaatgccgg atgtgacgcg ggttctacaa
    18421 cgttaccggg tgttctttct cgcctttctt aaacttcact tccccgagct caatacaacc
    18481 caccgggcag acgtgaccac aaagcagaca acccacacat ttctcggtat tacaatgcgg
    18541 tgtgcgggtt ttctcgctcc attccatcgc ctggtgaccg ccgtcgtaac aggaaatata
    18601 acagcgtcca cagccaacac atttgtcaag attgatacgg ggatagacaa tataactgcg
    18661 gtctaaatct tccgccggga caatattgtt attcgccaga cctaccatct cctgcaacga
    18721 atcaaatccc tgatcggcga gataatgcga taacccgctc gccatatctt ctacgatgcg
    18781 attgccgtac tgcataatgc cggtggtgac ctgtaacgtt gctgcgccga gcaataaaaa
    18841 ctccgccgca tcctcccagg tttcaatgcc gccgataccg ctgattggga aatcgcgcag
    18901 ttctggatgg gtgcgcattt gctgaatgaa gcgcagggcg atcggtttta ccgctttacc
    18961 ggaatatccg gaaatactcg atttaccgtt aacgatcggc ataccgattt tctgattaag
    19021 atcgatattg gtgatggatt taacggtgtt aattgccgca atgccatctg cgccgccgcg
    19081 cttcgccgcc agcgccactt cgcacatatc accgatattc ggcgtcatct tcgccagcat
    19141 tggcagcgtg gaaccgcgtt tcaccgcccg acaatatttt tctaccagca ccggactttg
    19201 cccgacatcg ctacccatcg catgagaagt catttgcgga caggagaagt tacactcgat
    19261 catatccgca ccagctcctc ccattgctgc tcattttccc ccatgatcga agcgattgga
    19321 tttgccccta tatttccaga catctgttat cacttaaccc attacaagcc cgctgccgca
    19381 gatattcccg tggcgagcga taacccagcg cactatgcgg atgccattcg ttataatg
//
LOCUS       DAZPEB010000062        19090 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZPEB010000062 DAZPEB010000000
VERSION     DAZPEB010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19090
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.063"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(9..3890)
                     /gene="entF"
                     /locus_tag="ACHURJ_001927"
     CDS             complement(9..3890)
                     /gene="entF"
                     /locus_tag="ACHURJ_001927"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFR1280892.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVDGLAQADTLRMRFTEDNGEVWQWVDDALTFELPEIIDLRTNIDPHGTAQALMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKMEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYQFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     ADVPDLTNLCYNAPLKPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYLLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTSV
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFVPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPLLPLSANGKLDRKALPLPELKAQAPGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQVARQVTPGQVM
                     VASTVAKLATIIDAEEDSTRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYI
                     DPQWSIIGIQSPRPNGPMHTAANLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIRATLNR"
     gene            complement(3887..4105)
                     /gene="ybdZ"
                     /locus_tag="ACHURJ_001928"
     CDS             complement(3887..4105)
                     /gene="ybdZ"
                     /locus_tag="ACHURJ_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFR1280893.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            complement(4108..5310)
                     /gene="fes"
                     /locus_tag="ACHURJ_001929"
     CDS             complement(4108..5310)
                     /gene="fes"
                     /locus_tag="ACHURJ_001929"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFR1280894.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     PIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDATAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5553..7793
                     /gene="fepA"
                     /locus_tag="ACHURJ_001930"
     CDS             5553..7793
                     /gene="fepA"
                     /locus_tag="ACHURJ_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFR1280895.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATRDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            7833..7931
                     /locus_tag="ACHURJ_001931"
                     /pseudo
     CDS             7833..7931
                     /locus_tag="ACHURJ_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7968..8588
                     /gene="entD"
                     /locus_tag="ACHURJ_001932"
     CDS             7968..8588
                     /gene="entD"
                     /locus_tag="ACHURJ_001932"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFR1280896.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKAEHLAGRIAAIYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(8710..8862)
                     /gene="hokE"
                     /locus_tag="ACHURJ_001933"
     CDS             complement(8710..8862)
                     /gene="hokE"
                     /locus_tag="ACHURJ_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFR1280897.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            8831..9100
                     /locus_tag="ACHURJ_001934"
     CDS             8831..9100
                     /locus_tag="ACHURJ_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1280898.1"
                     /translation="MTAARAYFVSMFILPPCEEGTTIQIVQVWVGSPRVDRNIVLGLH
                     LSRPQSKPETGKLQAPAVILPLLSKIAVYLYSKSCFGLLTPHLIN"
     gene            9315..10433
                     /locus_tag="ACHURJ_001935"
     CDS             9315..10433
                     /locus_tag="ACHURJ_001935"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFR1280899.1"
                     /translation="MPLPDFHVSEPFTLGIELEMLVVNPPGYDLSQDSSILIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQVTVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            10499..10747
                     /locus_tag="ACHURJ_001936"
     CDS             10499..10747
                     /locus_tag="ACHURJ_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFR1280900.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            10812..11180
                     /locus_tag="ACHURJ_001937"
     CDS             10812..11180
                     /locus_tag="ACHURJ_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFR1280901.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            11274..11926
                     /gene="nfsB"
                     /locus_tag="ACHURJ_001938"
                     /pseudo
     CDS             11274..11926
                     /gene="nfsB"
                     /locus_tag="ACHURJ_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
     gene            12034..13281
                     /gene="ybdG"
                     /locus_tag="ACHURJ_001939"
     CDS             12034..13281
                     /gene="ybdG"
                     /locus_tag="ACHURJ_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFR1280902.1"
                     /translation="MQDLISQVGDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGT
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESILNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPAGNDIRSLAGAFKQ"
     gene            complement(13349..14725)
                     /gene="pheP"
                     /locus_tag="ACHURJ_001940"
     CDS             complement(13349..14725)
                     /gene="pheP"
                     /locus_tag="ACHURJ_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFR1280903.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYICSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNEVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSHRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(14827..17970)
                     /gene="cusA"
                     /locus_tag="ACHURJ_001941"
     CDS             complement(14827..17970)
                     /gene="cusA"
                     /locus_tag="ACHURJ_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFR1280904.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQVGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMPFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKNDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIVEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGGAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(17982..>19090)
                     /gene="cusB"
                     /locus_tag="ACHURJ_001942"
     CDS             complement(17982..>19090)
                     /gene="cusB"
                     /locus_tag="ACHURJ_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFR1280905.1"
                     /translation="RKILFWYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVR
                     IDPTQTQNLGVKTATVTRGPLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVG
                     DKVQKGTPLLDLTIPDWVEAQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLI
                     ATQKIQTRFTLKAPIDGVITAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWL
                     VKDASQFTLTVPARPDKTLTIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWL
                     QLNTASEPMLLIPSQGLIDTGSEQRVITIDADGRFVPKRVAVFQASQGVTALRSGLAE
                     GEKVVSSGLFLIDSEANISGALERMRSESATHAH"
BASE COUNT         4620 a         5284 c         4864 g         4322 t
ORIGIN      
        1 atattaattt acctgtttag cgttgcgcga ataatcggcc caatttttac aaacgcccct
       61 ggagagataa tatccacatg cgcacaatcc tgacgataga tatccagctc ggcaatccag
      121 ggcgaccaag cgcgttcggg gctcatacct tcctgaagcg tgcgttcagc aacaaacagc
      181 gtcgctttac catcaaacgg tacgctatga gcagtcgtca gcagacgcac agcatcagcg
      241 tagttgcctt caatggtggt aaacaactcc gttgaagtac ttccctgctg tgccgccagg
      301 aaggcctcgc gttcgcggtt aatctccgcc agcacttccg ggtccagacc attagcctct
      361 ttttcctgcc agttttgcgt ttctggcggc caggtatcca gcaaaccaag aaatgccacc
      421 tgttcgccac gggcacgcag tcgcgccgca atgccctgcg ccagcgtacc gccaagggaa
      481 taccccagca ggtaataagg gccgtgcggt tgttgttcaa gtaacgttgc cagatgcgct
      541 tcacagactt catccagatt tgccgcggta tgcatggggc cattggggcg cggcgactga
      601 atgccgataa tcgaccattg tggatcgata taacgcgaga gcacgctaaa ctgccaggca
      661 aaaccggatg ccggatggaa acaaaacagc gtcgggccat taccttcacg caacggcaga
      721 atggtttcga atcccatacg ccgggtgctg tcttcttcag catcaataat cgttgccagt
      781 ttagcgacgg ttgacgcgac catcacttgc cccggcgtca cctggcgggc aacctgccga
      841 cttaactgcg ctgccagttt cattgccagt agcgaatgac caccaagcgc aaagaaatca
      901 gcatcggcat cctgcacgtc acaacccagc aacgacgcga atgccgcggc aataatcgtt
      961 tcactgcccg cttttggcgc acgccccggc gcttgtgcct tcagttcagg caacggtaag
     1021 gctttgcgat ccagcttgcc gttggcgcta agaggtaaca gtggaagttg cagcagaacc
     1081 acgggcacca tatgtggtgg caatgtttcg cgaagctgcg cctgtaatgc gctggtatcc
     1141 aacggcagac ctgattgcga caccagatag cccaccaact gacgcgcatc accaccggtg
     1201 gctgccgcct ggttaatcac acaggcgtgg gtaacggctt gttcgacatc cggcagcgcc
     1261 tgcatcacgc gatcgatttc gcccagttca atacgctgcc cgcgaatttt tagctgatcg
     1321 tcactgcgcc cgaggtactc caccgcgccg ttatccagcc agcgggcaac gtctccggta
     1381 cggtacatcc gttcaccagg gacaaaagga tcggcaataa agcggctggc ggtcagatcg
     1441 ggtcgtccaa gatacccctg cgccagttga atgccggtga gatagagatc acccgccaca
     1501 cccggcggca ccggatgcat catcgcatca agaatacgca gacccgtatt ccataccgga
     1561 taaccaatcg gcacactgct gccgcgcacc tgtgccagtt cctcgccaaa agccggatac
     1621 cagctgacat ctaccgccgc ttccgtcggg ccatatagat tatgcaacgg cacgctggtt
     1681 aactgttgcc attcgcggca taaatcagcc ggtaaggcct caccactaca gaaaacctgt
     1741 ttcaacgtcg cgcaactctg gcgagcggtt tgcggcgtca gcgatgcaac aaatgctgcc
     1801 agcatcgacg gcacaaagtg cgtggtcgtt acgccatatt cggcaaagaa ttgctgcata
     1861 gcgagcgggt cgcggtgcgc ttccggttca gccatcacca gtttagcccc ggcaataaac
     1921 ggccagaaaa actcccacac cgagacatca aaactgcacg gcgttttttg ggcaacgaca
     1981 tcttcgcctg taagcagata atgattttgc atccacaaca ggcggttaac gatagccgtc
     2041 tgcccgacca ttaccccttt cggcctgccg gtggagccag aggtaaagat gatataagcc
     2101 gtgtgatgcg gttgtgaaag ttgcagcggc gcactgccct gcggtttaag cggggcgtta
     2161 tagcaaaggt tggttaaatc tggaacatcg gcaaagcgcg gcagttgatc gtcggtggtg
     2221 attaacagcg acggacgcgc atcttccagc atcattttca ggcgatcgtc cggatagccg
     2281 gtatccagcg gtagccaggc cgcgccagct tcaactatcg catgtagcgc cagggtcaaa
     2341 aagaccgagc gcggtagcgc caccgccacg ctgtcgcccg gtttaacgcc acgctcacgc
     2401 agcagattcg ccagcgccac cacctgctcg cgcatttccc gatagctgaa ctggtaacgc
     2461 gcatctgcca gcgccggagc atccggtgtt tttgccgctt gttctgccac cagcgcgctc
     2521 agcgtggttt ctggaatctc aacctgagtg gcgttgatct gcgccagctg cgcatactca
     2581 cctggcagca taatatcgac atcgccgcac aacagcgccg gatccgcggc gaactgggca
     2641 atcagcattt tcaggcgttc agcatgctgg attaacgttg gctcatcgta acgctgttta
     2701 ttggcgagga tctcaatact caaatcaccg tgttcatccg ggaacagggc cagttcaagg
     2761 tcattaaccg gaccggttgc caaggtatgg gtttgcgcct gaacaccagg aatatccagt
     2821 tggtaatcaa ataccttgat attgagtacc ggaccaaaca gcggttcatc acctgccgct
     2881 cgcccgctgt cacggacaat ttgttcggca tcgtaacgtt gatgacgacg catttttttc
     2941 agttgtgctg ccagtcgggt tgccagttcc ggcagcgttt cttgcgccgc aatgtgaata
     3001 cccaacggca aaacgttgag cacgggtccg gtagccgtca gcgccgccga gcccagtcga
     3061 cgcataaaga taaatccagc ggcgtagtcc atgcgattgc acaatcgccc cagccacaaa
     3121 gccgtcagcg caagggctaa atcggtacgc tgcacacctg aaagttgcgt agccaactgg
     3181 cggaattccc cgtcggtaaa ttccattttc agacgtaaaa tatctgccga agcgctgcgc
     3241 cccggtaaag gtgccggaga aagtgacgcg ggcggcggca gttgacgacg ctgttctgcc
     3301 cagaatgccg catcacgctg ccaggcttcg ctttcgcggt attgctggta ctcttccact
     3361 acatcagcga aaggcgtaaa cggcgaagca ggcgttggtt caccacgcag ccatgtgcag
     3421 taaatattgg cgatctggcg ggtaatggcc gggaaactga agccatcgac cagcaaatgg
     3481 tgataacgct gataccagta ccagcggtta tccgccacct gtatcagctg atgaaagacc
     3541 agtggtttac cgctatcgac gcgcagatct tgttgcaaat ccgcctgcat taatgcctgc
     3601 gcagtaccgt gcggatcaat gttggttcgc aggtcgataa tttctggcag ttcgaacgtc
     3661 agcgcatcat cgacccactg ccagacttcg ccgttatctt ccgtaaaacg catccgcagc
     3721 gtatctgctt gcgctagtcc gtcaaccacc gcgcgggcca gtaatggcgc atcaacctct
     3781 ccggttaact caacgtaatg cgctacgctc caggcggagg gtaaatccga cagtttttct
     3841 gccatccaga tgccgggctg tgcggcgacc aaaggtaaat gctggctcat tgcgcctcct
     3901 gcaactgggt gaaattcgcc ggtgtcagag tacgccagtg ggcttcaagc cactgctggc
     3961 aggactcttg tgactgcggc tgacacacaa tgtcccagcc tgccggtaag gcgcattgtt
     4021 gcggccacag actgaattgc ccctgcgcat tgcgcaaaat gtaaaacgct ccctgtggat
     4081 catcgaaggg attactgaat gccatattca actcctgtca tggaaaagtg gttgccagag
     4141 gtcgattagc ccctgcatca agccaccgcg ccaacaaagc gcatcatgtc cgccgtcaac
     4201 ctggcgccag aaaatggatt cttttatggg gtgtaattgc gcatacagcg cctgattggc
     4261 ccgcataatc atcggctcgc gaatacccgc ttccagcaca atgcgcagac cttcggcgct
     4321 aacttcacca gctttaagct tttcaagtaa cacgccctct tgctgcccgc cccgatgcgg
     4381 ccaccagtac gatcctgact ggcttaatac acaaccaaaa cgttcaggcc agtgcagtcc
     4441 ggcatacagc gcggaaagtc caccaaaact ctgcccggca accacggtgc gatcggcgcg
     4501 atcgctaaaa ggggcaatag ctttcaccag gggtaataac tcttgctgta ctgcgagcca
     4561 gaaatccgca ttacacggca gttcgtgagc gcggtgcgtg gtgtcgatag cgtcgatcaa
     4621 cacatacacg gcgggaggaa gttgctgacg atgggtcagc gaagtcagca ctggccagac
     4681 gggcatactt tgcgcccaaa attcgccatc gagcaaaact gccagcgggc gttcttctgc
     4741 tgttgcatcg ccggtggtaa aaatccatac acgacgtgaa tttttcaacc gttcactttt
     4801 ccagataatt tctttggctg gtatttctgg cgcttgcgga caatcccatc ccggttgcag
     4861 aggcgcttgc ggcatttcga gtgcagaaac agcgtgccct cgcccgcctt tccagctttg
     4921 tggattcagc ggatcggcta ttgcctgggg taatagtttt cgccagcctt cacgtaattc
     4981 gaggcgatcg ggggatggtg cagaaaaaat gtcatcgcgt tcggtgggaa taaagcagta
     5041 gctgccgcgc cagttggcat tgagttgtgt cgtccactgc cagacatcag tacctgcaat
     5101 gcgctgcatc gactggggct ggctgttctg atgatgatcg gtcacaccag tgatgtagac
     5161 ccatacgcgc tttatcggcg agtattcttc agaaccttgg ggatcacgcc accagaaagt
     5221 gacctcaaac atttcgtcat tcagacgctg ccattccggg ccatgtttcg actgccacca
     5281 gctctcactt cctactttta acgccgtcac accataaccc catgtttact gtgcaatttt
     5341 tcattgattg cagaaatata ttgataatat tattgataac tatttgcatt tgcaatagcg
     5401 taatggcgcg ccgtgggaag cgcggacatt aattaaccaa ctgcactgcg tgtctttcag
     5461 gatcaaaggt tttcgcggta gcgggatgcg tcgtgttgag gacgaccatg cccgacagtt
     5521 gcaattcgtg gcaaaaatgc aggaataaaa caatgaacaa gaagattcat tccctggcct
     5581 tgttggtcaa tctggggatt tatggggtag cgcaggcaca agagccgacc gatactcctg
     5641 tttcacatga cgataccatt gtcgttaccg ccgccgagca gaacttgcag gcacctggcg
     5701 tttcgaccat caccgcagat gaaatccgca aaaacccggt tgcccgcgat gtgtcggaga
     5761 tcatccgtac catgccaggc gttaacctga ccggtaactc caccagtggt cagcgtggga
     5821 ataaccgaca gattgatatt cgcggtatgg gtccggaaaa cacgctgatt ttaattgacg
     5881 gcaaaccggt cagcagccgt aactcggtgc gtcagggctg gcgtggcgag cgcgataccc
     5941 gtggtgatac ttcctgggtg ccgcctgaaa tgattgaacg tattgaagtt ctgcgtggtc
     6001 cggctgcagc gcgttatggc aacggcgcgg cgggtggcgt agttaacatc attaccaaaa
     6061 aaggcagcgg tgagtggcac ggttcctggg acgcttattt caatgcgcca gaacataaag
     6121 aggaaggtgc cactaaacgc accaacttca gtctgaccgg cccgctgggc gacgaattca
     6181 gcttccgctt gtatggcaac ctcgacaaaa cccaggctga cgcgtgggat atcaaccagg
     6241 gccatcagtc cgcgcgtgcc ggaacgtatg ccacgacgtt accagccggg cgcgaagggg
     6301 tgatcaacaa agatattaat ggcgtggtgc gctgggactt cgcgcctctg caatcactcg
     6361 aactggaagc gggttacagc cgccagggta acctgtatgc gggcgatacc cagaacacca
     6421 actctgacgc ttacactcgc tcgaaatatg gcgatgaaac caaccgcctg tatcgccaga
     6481 actactcgct gacctggaac ggtggctggg ataacggagt gaccaccagc aactgggtgc
     6541 agtatgaaca cacccgtaac tcgcgtattc cggaaggtct ggcgggcggt accgaaggga
     6601 aatttaacga aaaagcgaca caggatttcg tcgatatcga tcttgatgac gtgatgctgc
     6661 acagcgaagt taacctgccg attgatttcc tcgttaacca aacgctgacg ctgggtacgg
     6721 agtggaatca gcaacggatg aaggacttga gttccaatac ccaggcgctg accggggcga
     6781 acaccggcgg cgctattgat ggtgtgagtg ccaccgaccg tagcccgtat tcaaaagcag
     6841 aaattttctc gctatttgcc gaaaacaaca tggagctgac tgacagcacc atcgtaacgc
     6901 cggggctgcg tttcgatcat cacagtattg tcggcaataa ctggagcccg gcgctgaaca
     6961 tatcgcaagg tttaggcgat gacttcacgc tgaaaatggg cattgcccgc gcctataaag
     7021 cgccgagcct gtaccagact aacccgaact acattctcta cagtaaaggt cagggctgct
     7081 atgccagcgc gggcggctgc tatctgcaag gtaacgatga cctgaaagca gaaaccagca
     7141 tcaacaaaga gattggtctg gagttcaaac gcgacggctg gctggcgggc gtcacctggt
     7201 tccgtaacga ttatcgcaat aagattgaag cgggctatgt ggctgtaggg caaaacgcag
     7261 tcggcaccga tctctatcag tgggataacg tgccgaaagc agtggttgaa ggtctggaag
     7321 gatcgttaaa cgtaccagtt agcgaaacgg tgatgtggac caataacatc acttatatgc
     7381 tgaagagtga aaacaaaacc acgggcgacc gtttgtcgat catcccggag tacacgttga
     7441 actcaacgct gagctggcag gcgcgggaag atttgtcgat gcaaacgacc ttcacctggt
     7501 acggtaagca gcagccgaaa aaatacaact acaaaggtca gccagcggtt ggcccggaaa
     7561 ccaaagaaat cagtccttac agcattgtgg gtctgagcgc gaccagggat gtgacgaaga
     7621 atgtcagtct gaccggcggc gtggacaacc tgttcgacaa acgtttgtgg cgtgcgggta
     7681 atgcccagac cacgggcgat ctggcagggg ccaactatat cgcgggtgcc ggggcgtata
     7741 cctataacga gccgggacgt acgtggtaca tgagcgtaaa cactcacttc tgatgctaac
     7801 gtcagattgt tgataaagtg cgcgtcgttc atgccggatg cggcgtgaac gccttatccg
     7861 gcctacaaaa atcttgccaa ttcaatatat tgcaggacca tgtaggcctg ataagcgtag
     7921 cgcatcaggc agtttagcgt ttgtcaccag tctctaatat ggtcgacatg aaaactacgc
     7981 atacctccct cccctttgcc ggacatacgc tgcattttgt tgagttcgat ccggcgaatt
     8041 tttgtgagca ggatttactc tggctgccgc attacgcgca actacaacac gctggacgga
     8101 agcgcaaagc cgaacattta gccgggcgga tcgctgcaat ttatgcgctg cgggaatatg
     8161 gctataaatg tgtgcccgca atcggcgagc tacgccaacc ggtgtggcct gcggaggtat
     8221 acggcagtat tagccactgc ggggctacgg cattggccgt ggtttcccgt caaccgattg
     8281 gcgttgatat cgaagaaatt ttttctgcgc aaacagcgac cgaattgaca gacaacatta
     8341 ttacaccagc agaacacgag cgactcgcag actgcggttt agccttttct ctggcgctga
     8401 cactggcatt ttccgccaaa gagagcgcat ttaaggcaag tgagatccaa actgatgcag
     8461 gttttctgga ctatcagata attagctgga ataaacagca ggtcatcatt catcgtgaga
     8521 atgagatgtt tgctgtgcac tggcagataa aagaaaaaat agtcataact ctgtgccaac
     8581 acgattaatt gacaacatct ggtacgattc gcccgcagcc atcactgacc gcgggcgaaa
     8641 gtgtaaagca ggtgccttac catcctgacc tgacaaccgg atatgcgggg attgctcccc
     8701 gcacaacggc tacttcttcg gttcgtaagc gagaacagcc ttaaactcaa tattacgttc
     8761 ctttacggtg aactcacaca gcgaatcccc gaccagaagc gtaaatccca gcaccgttaa
     8821 acacagcact atgactgccg caagggcata tttcgtcagc atgtttatat tgcctccatg
     8881 cgaagaaggg actaccatcc aaattgttca ggtttgggta ggtagcccca gggttgatag
     8941 aaatatcgtc ctggggcttc atctttccag acctcagagt aaacctgaaa ccggaaagct
     9001 tcaggcaccc gcagttatct tacccttgct tagcaaaata gctgtttatt tatacagtaa
     9061 gtcctgtttc ggacttctca ctccccattt gatcaactga agctgaaccc gtttttttct
     9121 gcatgcctca atcgtcaaaa ttcttcgcca cagtcagcac tgccataact tgtcacattg
     9181 caaaagtagc caaattgctt ctgtcataat cagttcacca tcaccaaaaa aaagaaacat
     9241 tccagaactt ccgcatctcg cccagccgct cccacttcta tactgaatag aaaacgccaa
     9301 cataagagaa acctatgcca ttacccgatt ttcatgtttc tgaacctttt acactcggta
     9361 ttgaactgga aatgctggtg gttaatccgc cgggctatga cttaagccag gactcttcaa
     9421 tactgattga cgcggttaaa aataagatca cggccggaga ggtaaagcac gatatcaccg
     9481 aaagtatgct ggagctggcg acggatgttt gccgtgatat caaccaggct gccgggcaat
     9541 tttcagcgat gcagaaagtc gtattgcagg cagccgcaga ccatcatctg gaaatttgcg
     9601 gcggtggcac acacccgttt cagaaatggc agcgacagga ggtatgcgat aacgagcgct
     9661 atcaacgaac gctggaaaac tttggttatc tcattcaaca ggtgaccgtt tttggtcagc
     9721 atgttcatgt tggctgcgcc agtggcgatg acgccattta tttgctgcac ggcctgtcac
     9781 ggtttgtgcc gcacttcatc gccctttccg ccgcgtcgcc atatatgcaa ggaacggata
     9841 cgcgttttgc ctcctcacga ccgaatattt tttccgcctt tcctgataat ggcccgatgc
     9901 cgtgggtcag taactggcaa caatttgaag ccctgtttcg ctgtctgagt tacaccacga
     9961 tgatcgacag cattaaagat ctgcactggg atattcgccc cagtcctcat tttggcacgg
    10021 tggaggttcg ggtgatggat accccgttaa cccttagcca cgcggtaaat atggcgggat
    10081 taattcaggc taccgcccac tggttactga cagaacgccc gttcaaacat caggagaaag
    10141 attacctgct gtataaattc aaccgtttcc aggcctgtcg ctatgggctg gaaggcgtca
    10201 ttaccgatcc gcacactggc gatcgtcgac cgctaacgga agacaccttg cgattgctgg
    10261 aaaaaatcgc cccttccgca cataaaattg gtgcatcgag cgcgattgag gccctgcatc
    10321 gccaggtcgt cagcggtctg aatgaagcgc agctgatgcg cgatttcgtc gccgatggtg
    10381 gctcgctgat tgggctggtg aaaaagcatt gtgagatctg ggccggtgac taagtaggga
    10441 attgccttac agaagaatat ccctgacaat ggtgttttaa cttacattta acaatgttat
    10501 gaaacaccct ttagaaacct tgactaccgc agcaggcatt ttgctgatgg ctttcctctc
    10561 ttgcctgctg ctgcccgccc ccgcactggg gctgacgctg gcacaaaaac tggtgaccat
    10621 gttccatctg atggatctta gccagcttta cactttattg ttttgtctgt ggtttttagt
    10681 gctgggcgct attgagtatt ttgttctgcg ctttatctgg cgacgctggt tctcgctggc
    10741 ggattaaacg tcgattatcg ccgtacttcg cggcatactt tgcttattct ccttcgccta
    10801 aaggaacgtt tatggataag caatcactgc acgaaacggc gaaacgcctg gcccttgagt
    10861 taccctttgt cgagctttgc tggccttttg gcccggagtt cgatgttttt aaaattggcg
    10921 gcaaaatttt tatgctgtcg tcggagctgc gcggcgtccc ctttatcaat ctgaagtccg
    10981 atccacaaaa atccctgtta aatcagcaga tatacccgag cattaagcca gggtatcaca
    11041 tgaataaaaa gcactggatt tcggtgtatc ccggcgagga aatctccgaa gcgttacttc
    11101 gcgatctgat caacgattcg tggaatctgg tggttgatgg tctggctaaa cgcgatcaaa
    11161 aaagagtgcg tccaggctaa agcggaaatc tatagcgcat ttttctcgct taccatttct
    11221 cgttgaacct tgtaatctgc tggcacgcaa aattactttc acatggagtc tttatggata
    11281 tcatttctgt cgccttaaag cgtcattcca ctaaggcatt tgatgccagt aaaaaactta
    11341 ccccggaaca ggccgagcag atcaaaactc tcctgcaata cagcccatcc agcaccaact
    11401 cccagccgtg gcattttatt gttgccagca cggaagaagg taaagcgcgt gttgccaaat
    11461 ccgctgccgg taattatgtg ttcaacgaac gtaaaatact tgatgcctcg cacgtcgtgg
    11521 tgttctgtgc aaaaaccgcg atggacgatg cctggctgaa gctggttgtt gaccaggaag
    11581 atgctgatgg ccgctttgcc acgccggaag cgaaagccgc gaacgataaa ggtcgcaagt
    11641 tcttcgctga tatgcaccgt aaagatctgc atgatgatgc agagtggatg gcaaaacagg
    11701 tttatctcaa cgtcggtaac ttcctgctcg gcgtggcggc tctgggtctg gacgcggtac
    11761 ccatcgaagg ttttgacgcc gccatcctcg atgcagaatt tggtctgaaa gagaaaggct
    11821 acaccagtct ggtggttgtt ccggtaggtc atcacagcgt tgaagatttt aacgctacgc
    11881 tgcgaaatct cgtctgccgc aaaacatcac cttaaccgaa gtgtaattct ctcttgccgg
    11941 gcatctgccc ggctatttcc cctcagattc tcctgatttg cataaccctg tttcagccgt
    12001 catcataggc tgctgttgta taaaggagac gttatgcagg atttaatatc ccaggttggg
    12061 gatttagcgg gtattgagat cgatcacacc acctcgatgg tgatgatttt cggtattatt
    12121 tttctgaccg ccgtcgtggt gcatattatt ttgcattggg tggtactgcg gaccttcgaa
    12181 aaacgtgcca tcgccagttc acggctttgg ttgcaaatca ttacccagaa taaactcttc
    12241 caccgtttag cttttaccct gcaggggatt atcgtcaata ttcaggcggt attctggctg
    12301 caaaaaggca ccgaagcggc agatattctg actacctgcg cgcagttgtg gatcatgatg
    12361 tatgcgctgc tttcagtctt ctcgttgctg gatgttattt tgaatctggc gcagaaattc
    12421 ccggcggcat ctcagttacc gctgaaaggg atatttcagg ggattaaact gatcggcgca
    12481 atcctggtcg gcattttgat gatctcgctg ctgattggtc agtcgccagc gattctgatc
    12541 agcggtcttg gtgcaatggc tgccgtgctg atgttggtat ttaaagatcc gattcttggt
    12601 ctggtggcag gtattcagct ttccgcgaac gatatgctga aactgggcga ctggctggag
    12661 atgccgaaat acggcgcgga tggcacggtg atcgatattg ggttaaccac cgtcaaagtg
    12721 cgtaactggg acaataccat taccactatt cccacctggt ctctggtttc tgactccttt
    12781 aaaaactgga gcgggatgtc agcatctggc gggcgacgta ttaagcgcag tatcagtatt
    12841 gatgtcacca gtattcgttt tcttgatgaa gacgaaatgc aacgtctgaa taaagcgcac
    12901 ttgttaaaac cttatttaac cagccgccat caggaaatta atgagtggaa tcgccagcaa
    12961 ggttctacgg agtcgatatt aaacctgcgc cgaatgacca atattggaac ctttcgtgcc
    13021 tatctgaacg aatatctgcg taaccatccg cggattcgta aagatatgac cttaatggta
    13081 cgccaactgg ctccaggtga taacggttta ccgctcgaga tctacgcgtt taccaacacc
    13141 gtggtgtggc tggaatatga aagcattcag gctgatatat tcgatcacat atttgcgatt
    13201 gtcgaagagt ttggtctgcg acttcatcag tcgccagccg gcaatgatat tcgctctctg
    13261 gcgggtgcat ttaagcagta attaaaaaaa ccgctctcat cgaatggatg agagcggaca
    13321 tcgttttgtc ggatgtagcg tgaatgcctt atttccgacg cagcgtttta aatgccacaa
    13381 acaggaatac aatccacacc ggcagcagga tcgctgacaa gcgcatatca tccatcgtgc
    13441 acatcagcag caaaatcatg ccgaggaagg caatgcaaag atagttgccg aacggataaa
    13501 gcagcgcctt aaactgtgtt tcacgtccct gacgtcgcat cgccgcgcga aaacgcagat
    13561 gcgccaggca gatcataatc cagttcaaca gcagcgttgc taccaccagc gccatcagca
    13621 gaccaaacgc tttttgcggc agcagatagt tgattaacac caccagcgaa gtgatcgctc
    13681 cggaaagcat cagcgagtta atcggcacgc cgcgatggct gacgcgagtc aaaaacttcg
    13741 gcgcattacc ctgcacagaa aggccaaaca gcatgcggct gttagagtaa accccgctgt
    13801 tatacactga cagcgatgct accagaatga cgaagttcag cgcagaagct acctcgttgc
    13861 tgtcgagatt atggaaaatc atcacaaacg ggctactgtt ggatttcact tccacccacg
    13921 gatagagcgc cagtaaaacc accagtgaac agatgtaaaa cagcaggatg cgatacacca
    13981 cctgatttac cgcttttgga atgctttttt ccggatcgcg cgcttcagcg gcagtaatcc
    14041 caatcagctc cagaccgccg aaggagaaca taattaccgc cagcgacaaa atcagcccat
    14101 tccagccggt ggcgaagaaa ccaccgtagc gccagaggtt gtcgatactg gctttctcgc
    14161 cgccgtgacc agaaaacagc agccacaggc caaagccgat cataccgatg attgccagca
    14221 ctttaatcaa cgcaaaccag aactcggttt cgccatataa gcgcacgttc accaggttaa
    14281 cggcgttgat gataataaag aaggcggcag cccaaatcca cgttggaaca tccgggaacc
    14341 agtactgcat atagatgccc gcagcggtca gctctgccat tcccaccagc acgaacatta
    14401 cccagtagtt ccagccagag aggaagcccg caaacggtcc ccagtattta taggcaaagt
    14461 gggcaaatga accggatacc ggctcttcga ccaccatctc gccgagctgg cgcataatca
    14521 ggaaagcgat gatcccggcg acgccgtagc ccagcaatac agccggaccc gccatctgaa
    14581 tcgccgggcc aatgccaaga aacagaccag taccaattgc gccacccagc gcaatcagtt
    14641 gaatatgacg gttatgtaat ccgcgatgaa gcgtcggctc ttgatttgac gcagtatctt
    14701 ccgatacggt tgacgcgttt ttcacgcctt tcccctgtgt gtcttttttg ttgaggggca
    14761 ccttttaaca tttagtgccc atcgtcgcaa gacacaatcc acacggttaa acggggtatc
    14821 ctgcttttat ttccgtaccc gatgtcggtg cagccacatc agcttatacg ccgccgggat
    14881 aataaacagc gacagcaaag gcgcggtgat catgccgcca atcatcggtg cggcaatccg
    14941 gctcatcacc tctgaaccag cccccgttcc ccacagaatc ggcagcagac cggcgataat
    15001 caccgccacc gtcatcgctt tcgggcgcac gcgcagtacc gcgccgtgat acaacgcctc
    15061 atccagcttc tgctcactga atgtttgcgg attattcaac gacggctcgg cctctatggc
    15121 gtgacgtaaa tacatcagca tcaccacgcc aaattcggcg gcgcccccgg caagggcaat
    15181 aaaaccggtg cccgttgcca cagaaagatg aaagcccatc caccacagca accagatgcc
    15241 gcccaccagc gcaaacggta cgctgctgat aatcagcaac gcttcgccca cccgacggaa
    15301 tgccagatac aacagcacga agatgatcat cagtgtcatc ggcaccatga gtttcagctt
    15361 atggttggcg cgctccagta gctcgaactg cccggagaat gccacgctgg tgcccggttt
    15421 taactgcact ttttcaacta tcgctttttg caaatcgtga accaccgaca ccatgtcacg
    15481 atcgcgggca tcgatataaa tccagctcgt cgggcgcgca ttctcggttt tcagcatcga
    15541 cggtccggta gagactttaa cgtcggccac gtctgccagg gtgatttgct gcttcatcgg
    15601 cgtcaggatc ggtagctggc gcagtgcctg cgggctatcg cgccagcttt gcggataacg
    15661 cagattaatg ggataacggg caatcccttc caccgtttcg ccaaccatcg ccccgcccac
    15721 cgcagaagtc acaaacaact gcacatccgc caccgtcata ccgtaacgcg cggctttttc
    15781 acggttaatc tcaacgttga tatagcgccc accttccaga cgctcggcaa gcgcagaagc
    15841 cacgcctggc accgttcgcg ctacttcttc aatttgctca gccatcgcgt cgatatccgc
    15901 cagcacagtg ccggaaactt taatgccgat ggggctttta atgccggttg agagcatatc
    15961 gatacggtta cgaattggcg gcacccacag attcgccagc cccggcagcc gcacggtgtt
    16021 atccagttcc tcaatgattt tgtccatcgt catgccaggc cgccactgat cctgcggctt
    16081 aagctggatg gtcgtttcaa ccatctcgag cggagcagaa tcggtggcgg tttccgcttt
    16141 cccggttttg ccaaataccc gcgccacttc aggtacgctc ataattagct tgtcgttttt
    16201 ttgcagcata ctcgccgcct ctgctgcgga aatccccggc agcgtcgatg gcatatacaa
    16261 caagtcgcct tcattgatct gcggtaaaaa ttccccgcca actttattga gcggccagag
    16321 aaccgtcagc accgaaagcg ctgccaccaa cagcgtggtt ttcggccagt gcagtacttt
    16381 cagcaataga ggatgataaa cacgaatcaa aaagcgattg agcgggttac tgctttccgg
    16441 cggaattttg ccacggatcc agtaacccat caggatcggg atcactacga tcgccagcag
    16501 cgccgcaccc gccatcgcat acgttttggt gaacgccagc gggccaaaca gacgcccttc
    16561 ttgcccttcc agggtgaaga ttgggataaa cgacagcgtg ataatcagca gactgataaa
    16621 cagcgccggc cccacttcaa cagatgcatc ggtaatcacc tgccagcgcg ttttattatc
    16681 cagcgtggcg tcaggatgct ggtgctgcca ctcttccagc cgtttatgcg cattctcgat
    16741 catgacgata gcagcatcga ccatcgcccc gacggcaatc gcaatgccac ccaacgacat
    16801 aatattggca ttcagtccct ggaagggcat gacaataaaa gcaatacaca accccagcgg
    16861 caacgaaata atcgccacca gcgccgagcg cacatgccag agaaacagcg cacagactac
    16921 cgccaccaca ataaactctt ccagcaactt gccgctgagg ttgtcgatgg cgcggtcaat
    16981 gagctggctg cgatcgtatg ttgtaactat ctccacgcct tccggcagac tacttttcag
    17041 cgtttccagt ttgtccttca cggcggcgat cacttctcgg gcgtttttgc cggagcgcag
    17101 gatcaccacc ccgcccgcca cttcgccttc gccgttcagt tcggcaatgc cccggcgcat
    17161 ctccgggcca acctggacct tcgcaacatc gcgcagataa acgggcacgc cattttcact
    17221 ggcttttaaa acgatgtgat taaagtcgtc gagcgtttgc agatagccgc tggcgcgcac
    17281 catatattcc gcttccgcca gttcgatcga cgaaccgccc gcttcctggt ttgaagcatc
    17341 cagcgcgctt tttacttcgg cgagactgat gccatactgc gccaggcgct ggggatcgat
    17401 aaccacctga tactctttca ccacaccgcc caccgacgcc acttccgcaa cgtcagggat
    17461 ggttttcagc tcatatttga gaaaccagtc ctgtaatgag cgtaaatcgg ccagatcgtg
    17521 cttaccgctg cgatccacca gtgcatattc atagatccag ccaacacccg tggcatctgg
    17581 ccccagctcg gcgctgactc ccgcaggcag cttaccctgt acctggttga ggtactccag
    17641 cacccgcgaa cgcgcccagt acggatcggt accatcttcg aaaatgacat acacataaga
    17701 gtcgccaaac tgcgagaaac cacgcacagt cttcgcgcca ggcaccgaca acatggtggt
    17761 ggttagcgga taagtcacct gattttcaac gatttgcggt gcctgcccgg gatagctggt
    17821 tttaataatc acctgcacat cggagagatc cggcagcgca tccactggcg tattaatgat
    17881 ggtccaggtg ccccagatgc taagaaacaa cgcgcccatc agcaccaaaa aacggttcgc
    17941 caccgagcga cgaataatcc attcaatcat tggttattcc ctcaatgcgc atgggtagca
    18001 ctttcagagc gcatccgctc cagtgcgcca gaaatattgg cttcggaatc aatcaggaaa
    18061 aggccgctgg aaaccacctt ttcaccttcc gccagaccag agcgtaatgc ggtgacacct
    18121 tgcgatgcct ggaaaacggc aacgcgtttc ggtacaaagc gcccgtcggc atcaatggta
    18181 atcacccgct gttcgctacc ggtatcaatc agcccttgtg acggaatgag cagcatcggt
    18241 tcgctggcgg tattgagttg caaccaggcg ttcatgcccg gttttagcgc ctcgtcggcg
    18301 ttgtcgactt ccagacgcag ctgcagcgtg cgggtcgcgg catccacgcc gggtagcagc
    18361 gtccatttgc ggatggtgag tgttttatcc ggtcgtgccg gaacggtgag cgtaaactgc
    18421 gaggcatctt tcaccagcca agcgatagac tccgggatcg cagcagtgac ccacaccggg
    18481 tccataccct gaattttcgc taccacgtta tctttggcga tattcattcc cgcgcgcaga
    18541 tcaaacgcgg tgatcacgcc atcaatgggc gctttgagcg taaagcgagt ctggattttt
    18601 tgcgtggcga tcaggcggcg aatatccgcc tccggcattc ccgccagtcg cagccgctca
    18661 agaatgcctt cagtctgggt cgccgtaccg ccggtttcgc gcagcagtaa atactcactc
    18721 tgcgcttcca cccagtcagg aatggtcagg tcgagaagcg gtgtgccctt ttgtacttta
    18781 tcgcccacgg taagcggata caccttgtcg ataaacccgg cagcgcgagc ctgcacaatg
    18841 gcatactgat actcgttgta actgacattc gccgggaaac tctgggcaaa agtcagcggt
    18901 ccgcgcgtga cggtagccgt tttcaccccc agattctgcg tctgagtcgg gtcaatgcgc
    18961 acaccagacg cagaactctc ttcatcggca tatttcggca ccagatccat atccataaac
    19021 ggcgatttac ctggtttatc gaaccgcgta ttggggtaca tcgggtcgta ccagaataag
    19081 attttacgtt
//
LOCUS       DAZPEB010000063        18961 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZPEB010000063 DAZPEB010000000
VERSION     DAZPEB010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18961
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.064"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..745)
                     /gene="fsr"
                     /locus_tag="ACHURJ_001943"
     CDS             complement(<1..745)
                     /gene="fsr"
                     /locus_tag="ACHURJ_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFR1280906.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSVGAVLLAAALVGTSSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLTAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISS"
     gene            963..2615
                     /gene="ushA"
                     /locus_tag="ACHURJ_001944"
     CDS             963..2615
                     /gene="ushA"
                     /locus_tag="ACHURJ_001944"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFR1280907.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDVEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQAPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVTKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(2652..3131)
                     /gene="ybaK"
                     /locus_tag="ACHURJ_001945"
     CDS             complement(2652..3131)
                     /gene="ybaK"
                     /locus_tag="ACHURJ_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFR1280908.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMAAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(3335..4129)
                     /locus_tag="ACHURJ_001946"
     CDS             complement(3335..4129)
                     /locus_tag="ACHURJ_001946"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFR1280909.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMTPLPTRLLKKLKNADALIVEADVSTSDTPFANLPTCEALEERINEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQITMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            4267..4608
                     /locus_tag="ACHURJ_001947"
     CDS             4267..4608
                     /locus_tag="ACHURJ_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFR1280910.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSTL
                     INNNRKLTTEMAFRLAKVFDTIVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(4823..7327)
                     /gene="copA"
                     /locus_tag="ACHURJ_001948"
     CDS             complement(4823..7327)
                     /gene="copA"
                     /locus_tag="ACHURJ_001948"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFR1280911.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARVVTAEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALI
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNPALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKVVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            7589..8521
                     /gene="glsA"
                     /locus_tag="ACHURJ_001949"
     CDS             7589..8521
                     /gene="glsA"
                     /locus_tag="ACHURJ_001949"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFR1280912.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAVVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENIEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGLN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            8524..9816
                     /locus_tag="ACHURJ_001950"
     CDS             8524..9816
                     /locus_tag="ACHURJ_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFR1280913.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVAHAFGAYAVQFLHEGSQEEHLILLYALGSIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            9847..9944
                     /locus_tag="ACHURJ_001951"
                     /pseudo
     CDS             9847..9944
                     /locus_tag="ACHURJ_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9941..10348
                     /gene="cueR"
                     /locus_tag="ACHURJ_001952"
     CDS             9941..10348
                     /gene="cueR"
                     /locus_tag="ACHURJ_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFR1280914.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(10349..10807)
                     /locus_tag="ACHURJ_001953"
     CDS             complement(10349..10807)
                     /locus_tag="ACHURJ_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFR1280915.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(10804..11721)
                     /locus_tag="ACHURJ_001954"
     CDS             complement(10804..11721)
                     /locus_tag="ACHURJ_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFR1280916.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            11867..12544
                     /gene="fetA"
                     /locus_tag="ACHURJ_001955"
     CDS             11867..12544
                     /gene="fetA"
                     /locus_tag="ACHURJ_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFR1280917.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNRQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            12531..13310
                     /gene="fetB"
                     /locus_tag="ACHURJ_001956"
     CDS             12531..13310
                     /gene="fetB"
                     /locus_tag="ACHURJ_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFR1280918.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDIFWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(13373..14227)
                     /gene="cnoX"
                     /locus_tag="ACHURJ_001957"
     CDS             complement(13373..14227)
                     /gene="cnoX"
                     /locus_tag="ACHURJ_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFR1280919.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(14288..15097)
                     /gene="ybbO"
                     /locus_tag="ACHURJ_001958"
     CDS             complement(14288..15097)
                     /gene="ybbO"
                     /locus_tag="ACHURJ_001958"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFR1280920.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNNMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(15087..15710)
                     /gene="tesA"
                     /locus_tag="ACHURJ_001959"
     CDS             complement(15087..15710)
                     /gene="tesA"
                     /locus_tag="ACHURJ_001959"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFR1280921.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            15681..16367
                     /gene="ybbA"
                     /locus_tag="ACHURJ_001960"
     CDS             15681..16367
                     /gene="ybbA"
                     /locus_tag="ACHURJ_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFR1280922.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            16364..18775
                     /gene="ybbP"
                     /locus_tag="ACHURJ_001961"
                     /pseudo
     CDS             16364..18775
                     /gene="ybbP"
                     /locus_tag="ACHURJ_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
BASE COUNT         4380 a         4925 c         5142 g         4514 t
ORIGIN      
        1 agctgctgat gctcgccata tagaaatatt tcgagaaaat gaggattaac agaatgctga
       61 ctgccagtac cactttgttg cgcggcagtg gattgattat cgtcgctttg ggttttcctt
      121 tattcattcg gtgctgtgcc gagtaccaac ggctaatttg cgccaacacc acgatcgcca
      181 gcagtgccgc aagcacaaac caggcaacgt tgcctttgcc ataaggcgcg ataatcaccg
      241 ccgtcagcaa gggtcccagg gaactgccaa agttgccgcc gacctgaaag atagattgcg
      301 ccaggccatg ccgcccgccg gaagccatac gggctacgcg agaagattcc ggatgaaaga
      361 ccgatgaact ggtaccgacc agcgccgccg ccagcagaac tgcgccaaca ctgcccgcca
      421 gcgcaagcag caccagacca cttaaggtaa agcacatgcc aattggcaac gaccacggca
      481 tcggatattt atcggtccag tagccgacca ctggttgcag tagcgaagag gcgagctgga
      541 aggtgagggt tatcatgcca atctgcataa atgtcagaga aaattctgac tgaagcagag
      601 gataaatcgc cagaatcagc gattggatca tgtcgttcag cagatgtgag aggctgatag
      661 cacctaaaat accaaacgat gttcgggcct tggtcgttga cgcagccgcg cccgccacag
      721 gctgggtttg ttcactcatt gccataggaa agtcactttt tcagggttgc gatgtaaaga
      781 atgatcttat ttgtgattat tacccgacta acatacctgt aagcgtcgcc tgaaggaagt
      841 ctcaacgccg aatacagaat ttctaatctg gatgcagatt tatcttcacc ggacgcagac
      901 ttgtctatta tgttgcgtca tactattttt caacacgttg aaatcaggtc agggagagaa
      961 gtatgaaatt attgcagcgg ggcgtggcgt tagcgctgtt aaccacattt acactggcga
     1021 gtgaaactgc tctggcgtat gagcaggata aaacctacaa aattacagtt ctgcatacca
     1081 atgatcatca tgggcatttt tggcgcaatg aatatggcga atatggtctg gcggcgcaaa
     1141 aaacgctggt ggatggtatc cgcaaagagg ttgcggctga aggcggtagc gtgctgctac
     1201 tttccggtgg cgacattaac actggcgtgc ccgagtctga cttacaggat gccgaacctg
     1261 attttcgcgg tatgaatctg gtgggctatg acgcgatggc gatcggtaat catgaatttg
     1321 ataatccgct caccgtatta cgccagcagg aaaagtgggc caagttcccg ttgctttccg
     1381 cgaatatcta ccagaaaagt accggcgagc gcctgtttaa accatgggcg ctgtttaagc
     1441 gtcaggatct gaaaattgcc gtcattgggc tgacaaccga tgacacagca aaaattggta
     1501 acccggaata cttcactgat gtcgaatttc gtaagcccgc cgatgaagcg aagctggtga
     1561 ttcaggagct gcaacagaca gaaaagccag acattattat cgcggcgacc catatggggc
     1621 attacgataa tggtgagcac ggctctaacg caccgggcga tgtggagatg gcacgcgcgc
     1681 tgcctgccgg atcgctggcg atgatcgtcg gtggtcactc gcaagctccg gtctgcatgg
     1741 cggcagaaaa caaaaaacag gtcgattacg tgccgggtac gccatgcaaa ccagatcaac
     1801 aaaacggcat ctggattgtg caggcgcatg agtggggcaa atacgtggga cgggctgatt
     1861 ttgagtttcg taatggcgaa atgaaaatgg ttaactacca gctgattccg gtgaacctga
     1921 agaagaaagt gacctgggaa gacgggaaaa gcgagcgcgt gctttacact cctgaaatcg
     1981 ctgaaaacca gcaaatgatc tcgctgttat cgccgttcca gaacaaaggc aaagcgcagt
     2041 tggaagtgaa aataggcgaa accaatggtc gtctggaagg cgatcgtgac aaagtgcgtt
     2101 ttgtacagac caatatgggg cggttgattc tggcagccca aatggatcgc actggtgccg
     2161 actttgcggt gatgagtgga ggaggaattc gtgattctat cgaagcaggc gatatcagct
     2221 ataaaaacgt actgaaagtg cagccattcg gcaatgtggt ggtgtatgcc gacatgaccg
     2281 gtaaagaggt gattgattac ctgaccgccg tcgcacaaat gaagccagat tcaggtgcct
     2341 acccgcaatt tgccaacgtt agctttgtga cgaaagacgg caaactcaac gacctgaaaa
     2401 tcaaaggcga accggtcgat ccggcgaaaa cttaccgtat ggcgacatta aacttcaatg
     2461 ccaccggcgg tgatggctat ccgcgccttg ataacaaacc gggctatgtg aataccggct
     2521 ttattgatgc cgaagtgctg aaagcgtata tccagaaaag ctcgccgctg gatgtgagtg
     2581 tttatgaacc gaaaggtgag gtgagctggc agtaatccga aagtgccgga tatttgcatc
     2641 cggcacaatg cttaatcgcg gcgggcgata tcagcaaatt tggcatcgag gatcttcgcc
     2701 agatcgcctg ccgccagttc gatatccagt ccgcgcttgc cgccagaaac ataaatagtg
     2761 gcaaattctt gtgcgggggc gtcgataatc gtcggcagac gttttttctg ccccagtggg
     2821 ctaatccccc caaccaggta tcccgtcgaa cgctgcgcgg ccatcggatc ggccatctca
     2881 actttcttgg cacccagcgc ttttgctact tttttaagat ccagttgacc tgcgaccggc
     2941 gtaacggcca ctgccagatg tttcatatca ccgttcactg ccaccagtag cgttttgtag
     3001 acttgatccg gattcaaacc taattttttg acgacttcat cgccaaaatt ggtttcagcc
     3061 ggatcgtgct cgtaggtatg gatttgaaac gaaatcttgt ttttttcgag taatttaact
     3121 gcgggtgtca tggtaatttt cctgccttaa acaaaaaaac gaagcaagca tacgcctgat
     3181 ggcggtccaa aaaaaagagt catcttgcct aagagtattg gcaggatggt gagattgagc
     3241 gacaatcgag ttacaccgtc gcttaaagtg acggcataat aataaaaaaa tgaaattcct
     3301 ctttgacggg ccaatagcga tattggccat ttttttagcg caacatttgc ggcaaattcc
     3361 cttctccata caggtgtagt gcaccgaccg cgaccacata tcgccccggc ggcatggcgc
     3421 gtaatttatc ccgccaggcg agatttcgct gatgcatcag cacatcgtac agcgactgac
     3481 tgaacgtatt gggcagcgtt atatcattat tttgcggagg tgcattcagc caccagctca
     3541 tcatttgttg cagcaaccgt gcgttggtat gccagtgggt cagcgtatcg tccagcagcg
     3601 ccagtccttt gtcagggagc tggagcaaca tggtaatctg gttttcagcc ccttccagtt
     3661 caatcacggg tttatgttgt tgcttcgccg cctgcaataa ctggtaatcg ataccgtatt
     3721 ctgcccgcag ccccagtttt tgtgcctgcg tcgcctgaag aaccatcgcg atttgccaca
     3781 gcggttgagt agaaaagagc gacggagaaa tgcccatctc ctggctaatg tgctgcagat
     3841 tttggagttg ctcctcgtta atgcgctctt ccagcgcctc gcaagtaggc aaattagcaa
     3901 aaggcgtatc gctggtggaa acatccgcct cgacgatcag cgcatcggcg tttttgagct
     3961 ttttgagcaa acgggtgggc aggggagtca tatcgtggct acccatatga atactgccaa
     4021 tcagatgaaa atggcgattg ccgggaaggg tgatatcgat ggcaggccag gtgtaatgat
     4081 taccgcgcag cgcggcccaa agcgttttta cccggtacaa cagatccata cgacctccct
     4141 ttgtgaaata tcatgctagc gcgcggtgag ggatggcgca aatggagttg gggacttgtt
     4201 taatgtgttt gtatgattca gtatgttctt gcatcgctat tcacagggaa gcaacagtta
     4261 aaaaccatga aacaggcaac aagaaaaccg acgacacccg gagatattct tctctatgaa
     4321 tatctggaac cgctcgattt gaaaatcaat gagttagcag agttgctgca tgttcatcgt
     4381 aatagcgtca gtacactgat caataataat cgtaaactca ccactgagat ggcattccgt
     4441 ctggcgaaag tttttgatac catagtcgat ttttggctaa acctccaggc ggcagttgat
     4501 ctttgggaag ttgaaaacaa catgcgcacc caggaagaat tgggacggat tgaaacagtg
     4561 gctgaatatt tggcacgccg tgaagagcgt gcaaaaaagg tcgcgtaagg ccaaaacatc
     4621 gcttcatctc gtaggccgga taaggcgttc acgccgcatc cggcatccga gcaccattgc
     4681 ctgatgcgac gcttacccgt cttatcaggc ctacaaacct gtgcctgaac cgtaggccgg
     4741 ataaggcgtt tacgccgcat ccggcagtca tgcgtcgatg ccaaatgcgc cgccctaaag
     4801 cagcgcatcc gcaatgacgt acttattcct tcggtttaaa ccgcagcaac cggttggcgt
     4861 tactcactac ggtaatcgac gagagcgcca ttgccgctcc ggcaactacc gggttaagca
     4921 gtgttccagt aaacggccac aaaataccag cggcgaccgg aataccaatg ctgttgtaga
     4981 taaacgcacc gagcaggttc tgcttcatat tgcgcagcgt tgcgcgggaa atcgcgagcg
     5041 catctgcaac acccatcagg ctatggcgca tcagggtaat cgccgcggtt tcaatggcaa
     5101 catcactgcc gccacccatc gcaataccga catccgcctg agccagcgct ggcgcgtcgt
     5161 taatgccgtc gcccaccatt gccacctgac gtccttcact ttgcagacgt ttgatcgctt
     5221 cggctttacc gtctggcagc accccggcga ttacctcatc aatccctgct tctttggcga
     5281 tcgcattggc ggtggttggg ttatccccgg tcaacatcac cagacgatat cccgctttat
     5341 gcaggcgttg cagcgccgcc acgctatcac tacgcaacgg atcgcgtaca gccagcaggg
     5401 ctactacttt cccgtcaatc gccagcagca caggcgttgc tccttgcgat gcctgagctg
     5461 aaatatcggc ttcaatcgct ttagtatcga cttgctggtc atttaacagc gccggattac
     5521 ccagcaataa cgcatgacct tcagcttcac cgcttacgcc cagcccgcgc aatgtgcgga
     5581 aaccgttgac ctgcggcagc tgcatatcgc ctgctttatc gaggatcgct cgtgccagcg
     5641 gatggctgga accttgctcc agcgccgccg ccagacgcaa tgcttgcgct tcatcaacat
     5701 cagcaaatgt ttttactgcg acaacctgcg gcttcccttc ggtcagcgtc ccggttttat
     5761 cgaacacgac ggtatcgagt gtactggcgc gttgcagcgc gtcggcgtcc cgcaccagca
     5821 cgccaaactc agccgcccgc ccgacgccgg aaataatcga cattggcgtc gccagcccca
     5881 gcgcacacgg acaggcaata atcagcaccg tggtggctat caccagggta tagacaatct
     5941 gcggtgccgg accaaagaaa taccagattg ctgcactgat cagcgcaatc accactacta
     6001 ccggcacaaa tacggctgag attttatccg ccagctgacc gatttctggc ttgctgctct
     6061 gggcctggcg caccatgcga ataatccttg acagcgtggt atggctgcca accgcactgg
     6121 cgcgaaatag cacgctaccg tcctgtacca ctgtcccggc atggacgctt tcgccttcgc
     6181 ctttttgctg cgggattggt tcgcccgtca gcatcgcttc atccagccat gcttcgccct
     6241 gggtaatctc gccatctacc ggcacgcgat cgccggtcgt caggcgcagt aacatccctg
     6301 gctgaacttc cgccagcggc acgctttttt caccctctgc ggtgaccact cgcgccgtcg
     6361 gtggggttaa atcgagtaat ttctccagcg ccttagaaga acgctgacgc gcgcgcgctt
     6421 ccagcatatg gccgagatta atcagaccga taatcatcgc actggcttca tagtaaagat
     6481 gccgcgcttc catcgggaac cactgcggcc acaggttgac gctcatcgaa tagagccacg
     6541 ccacacccgt acccagcgcc accagcgtat ccatcgtcgc cgcaccgttc agcaggcttt
     6601 tccacgcgct gcggtaaaaa tgcccgccgg cgaaaaccat taccgccagg gttatcaggc
     6661 cgataactaa ccacaggctg cggttgtcag cggtgaccat catgttatcg ccgatcatcc
     6721 cccagaccat taccggaata cccaccgcca gtgcgacaat cgcctgccag cggaagcgct
     6781 tcatcgtagc gacggcggtt tcctgctggc gctcgcggcg tttcgcgtca tcttcaatcg
     6841 cttccgcgcc gtagcccgct ttttccaccg cctgcactaa atcttgtggg gaggcactgc
     6901 ccatcaccag cgcagtacgc tccgccaggt ttacccgtgc ctgagtgacg cccggtacgc
     6961 tttgcagcgc attttgtacg cgggtgacac agctggcgca gctcatgccg ctcagcagca
     7021 actgctggct gtcatcgtca tcggcggtcg ctgccggaag cgcctcagaa accgctgtca
     7081 gtgcttccga cgggattgat gactccgcca gcggtttagc ctttgggtgg cttacagatg
     7141 cgtcataacc cgcttgtttg atggtttcga tcagctgttc tgcactggca gtcccggtaa
     7201 cgtgcgcttc agtgatagac acatccgctt gttccacatc cgggcgctgt tcgagacttt
     7261 ctttcacgcg tttaacgcag tgaccgcagg acaggccgtc cagggtcagg tcgatagttt
     7321 gtgacataaa acactccttt aagacagttt tgactggctg tgataaaggt taaaccttcc
     7381 agcaagggga aggtcaagaa attaataaac caggcgggta aaagtccgta aagattaaaa
     7441 aatcggctcg atttgcatca ggattagaca tttatttctt tgttttcctg tagttaagtt
     7501 gctggtgcta agttaaatca ggatgcctga aaatcggcac cggggtgagg aattacctcc
     7561 cgcatctata aaaaggagtt aacaaaagat gttagatgca aacaaattac agcaggcagt
     7621 ggatcaggct tacacccaat ttcactcact taacggcgga caaaatgccg attacattcc
     7681 ctttctggcg aatgtaccag gtcaactggc ggtagtggct atcgtgacct gcgatggcaa
     7741 cgtctatagt gcgggtgaca gtgattaccg ctttgcactg gaatccatct cgaaagtctg
     7801 tacgttagcc cttgcgttag aagatgtcgg cccgcaggcg gtacaggaca aaattggcgc
     7861 tgacccgacc ggattgccct ttaactcagt tatcgcctta gagttgcatg gcggcaaacc
     7921 gctttcgcca ctggtaaatg ctggcgctat tgccaccacc agcctgatta acgctgaaaa
     7981 tattgaacaa cgctggcagc gaattttaca tatccaacag caactggctg gtgagcaggt
     8041 agcgctctct gacgaagtca accagtcgga acaaacaacc aacttccata accgggccat
     8101 agcctggctg ctgtactccg ccggatatct ctattgtgat gcaatggaag cctgtgacgt
     8161 gtacacccgt cagtgctcca cgctcatcaa tactgttgaa ctggcaacgc ttggcgcgac
     8221 gctggcggcg ggtggtctga atccgctgac gcataaacgc gttcttcagg ccgacaacgt
     8281 gccgtacatt ctggccgaaa tgatgatgga agggctgtat ggtcgctccg gtgactgggc
     8341 gtatcgcgtt ggtttaccgg gcaaaagcgg tgtaggtggc ggtattctgg cggtcgtccc
     8401 tggcgtgatg ggaattgccg cgttctcacc accgctggac gaagaaggta acagtgttcg
     8461 cggtcaaaaa atggtggcat cggtcgctaa gcaactcggc tataacgtgt ttaagggctg
     8521 atcatgatga acacggaagg taataacggt aacaaacccc tcggtctatg gaatgtcgtt
     8581 tccatcggta ttggggcaat ggtgggggcg gggatcttcg cgctgctggg gcaggctgcg
     8641 ttgctaatgg aagcctcgac ctgggtcgcc tttgcttttg gcggtattgt ggcgatgttt
     8701 tccggttatg cctatgcgcg tctgggggcg agctatccca gcaatggcgg cattatcgac
     8761 ttctttcgtc gcggattagg caacggcgtc ttttcgctgg cgctctcgtt actgtacctg
     8821 ttgacgctgg cggtgagcat cgccatggtc gcccatgctt ttggcgctta tgccgtgcag
     8881 tttttgcatg aaggcagcca ggaggagcac cttattttgc tctacgcgtt ggggagcatt
     8941 gcggtgatga cgcttttcaa ctccttaagc aaccatgcgg tagggcggct ggaagtgatc
     9001 ctcgtcggca ttaaaatgat gatcctgtta ttgctgatta ttgccggtgt ctggtcgctg
     9061 caaccagcgc atatttccgt ctctgcgccc cccagctccg gtgcgttctt ctcctgtatt
     9121 gggataactt tccttgccta tgcgggcttt ggcatgatgg cgaacgcggc ggataaagtg
     9181 aaagatccgc aggtcattat gccacgggcg tttctggtgg cgattggcgt taccacgttg
     9241 ctttatatct cgctggcact ggttttgctt agcgatgtat cggcattaga gttagaaaaa
     9301 tatgccgata ccgccgtagc gcaggctgct tctccgctgc tcggacatgt gggttatgtg
     9361 atcgtcgtca tcggcgcttt actggcgacg gcttcagcca ttaacgcgaa cctgttcgcc
     9421 gtgtttaaca tcatggacaa catgggcagc gaacgcgaac tgccgaagct aatgaataaa
     9481 tccctgtggc ggcagagtac ctggggcaac atcattgtcg tggtgttgat tatgctgatg
     9541 acggcggcac tgaatttagg ctcactcgcc agcgttgcca gcgccacctt tttgatttgc
     9601 tacctggcgg tgtttgtggt ggcgatccgc ctgcgtcatg atattcacgc ctcgttgccg
     9661 attcttatcg ttggtacgtt ggtgatgttg ttggtgatcg ttggctttat ctacagtctg
     9721 tggtcccagg gtagccgtgc gttgatatgg attattggct cactcttact cagccttatt
     9781 gtggcaatgg tcatgaagcg caataaaacc gtataacatc tctctgtgcg cagtacttcc
     9841 tgtattattg tggtggcggt cgatattcgc actggcaaaa aaacgtgctt gaatatctgt
     9901 tgaaaccctt taacaaagca caggaggcgt tgcgcgaacg atgaacatca gcgatgtagc
     9961 aaaaattacc ggcctgacta gcaaagccat tcgcttctat gaagagaagg ggctggtgac
    10021 gccgccgatg cgcagcgaaa acggttatcg cacctacacg cagcagcatc tcaacgaact
    10081 gaccttactg cgccaggcac ggcaggtggg ctttaacctg gaagagagcg gcgagctggt
    10141 gaatctgttt aacgacccgc agcggcacag cgccgacgtc aaacggcgca cgctggagaa
    10201 ggtggcggag atcgaacgac acattgagga gctgcaatcc atgcgcgacc agctgctggc
    10261 actggcgaat gcctgccctg gcgatgacag cgccgactgc ccgattatcg aaaatctctc
    10321 cggctgctgt catcatcggg cagggtgatt aagacgagac ggctcggata tgtagggtta
    10381 tcccttctat cgcaatgact tcaacatgcg tacctgcacc gagatcctcg ctggcgctga
    10441 caggccatga actgtcaccg acgcgcatat gaccgcgccc gttgaccagc ggagattcca
    10501 gcacaaaacg tcggccaatc agctgctgcc cgcgctggtt taaatgacta tcgctgtgct
    10561 tttgttcgcg cacccgccgc gacaaccatt tccaccacag ccaggcggcg agcagcgtca
    10621 ggatggcaaa cattacccct tgccactccc aacccagcgg caccagccag accaccaggc
    10681 cggtaatcac cgctgccacg ccgctccaca acaaataacc atttccgccc agcatctcgg
    10741 ctgccagcag caaaccgccg agactgagcc agaaaatatg tggatgaacg accattaact
    10801 ccatcatggc tgagtccgtt tgttggcgct gtctttcacc agttcggcaa tcccggcaat
    10861 cgaccccatc aggctgctgg cctctaatgg catcatcact actttgctgt tactggagga
    10921 accgatctgc tgtaacgctt cggtgtattt ctgcgctacg aagtagttca ccgcctgaat
    10981 atcaccggag gcgatagctt cagacaccat tttggtggcg cgggcttctg cttcggcgga
    11041 acgttcacgc gcttcagcct gtaaaaacgc cgactgacgt tcgccttccg ctttcaggat
    11101 ttgcgactgt ttttcacctt cggctttgag gatttccgcc tgacggatcc cttccgcttc
    11161 aagaatgtaa gcgcgtttgg tacgttccgc tttcatctgc gcgttcattg aagagataag
    11221 ctctgccggt gggcgcacgt cgcgaatttc aatacgggtg actttaatcc cccacgggtt
    11281 ggtggcttca tcgacaatac gcagcaggcg tgagttgatg ctgtcgcgct gagagagcat
    11341 ttcgtcaagt tccattgaac ccaacacggt acggatgtta gtcatggtca ggttgatgat
    11401 cgccagctcc agattgctga cttcataggc agcgcgtggc gcgtcaatca cctgaataaa
    11461 acagactgcg tcgatggtaa cgttggcgtt atctttcgag ataacttcct gggaagggat
    11521 atcgagcact tgctccatca tattgatctt gcgaccaatg cgatccataa acggcaccac
    11581 cagactgagc cccggctgta acgttttggt atagcgacca aaacgttcta ccgtccattg
    11641 atagccctgc ggcacgattt tgacgcccgc gccgacaatg accagcgcga caaaaatgag
    11701 aatcgggata aagataagca tcggaaaaac ctcctgttgt accgtccata atcagcaaaa
    11761 ttgctgcttg attaaacaaa ttatacctga ttactgaaag agagttcccc cttattcctg
    11821 cgaaggataa actgttttta gtaaaaatca gaaaaaggga acagcgatgc aggaaaatag
    11881 tcctttgctt cagctacaaa acgtaggata tctggcgggt gatgcgaaga ttcttaataa
    11941 catcaatttt tcgctgcgtg ctggcgaatt taagttaatt accggtcctt ctggttgtgg
    12001 caaaagtacg ctgctaaaaa tagttgcttc attgatcagc ccaaccagcg gaacgttact
    12061 gtttgaaggt gaggatgtca gcacactaaa gccagaaatc taccgccaac aagtctctta
    12121 ctgcgcccag acaccgacgc tgtttggcga tacggtatac gataatctga tctttccctg
    12181 gcagatccgt aaccggcagc ctgacccagc catttttctc gattttctcg aacgcttcgc
    12241 cttgccggac agcattttga cgaaaaatat cgccgagcta tctggtggtg aaaaacaacg
    12301 catctcactg attcgtaacc tgcaatttat gccgaaggtt ttattgctgg atgaaatcac
    12361 cagtgcgctg gatgaaagta ataaacataa cgttaatgag atgatccatc gttatgtgcg
    12421 cgagcaaaat attgccgtgc tgtgggtgac acacgataaa gacgaaatta atcatgcgga
    12481 taaagtgatt acactgcaac cgcatgccgg agaaatgcag gaagcacgct atgaactcgc
    12541 ataatattac taacgaatca ttggcactgg cattaatgct ggtggtggtg gcaatcttaa
    12601 ttagccataa agaaaaactg gcgctggaga aagatatttt ctggagcgtc gggcgcgcga
    12661 taattcagct gattattgtc ggctatgtgc tgaagtatat tttcagcgtg gatgatgcca
    12721 gcctgacatt attgatggtg ttatttatct gctttaatgc ggcgtggaac gcgcaaaaac
    12781 gcagtaaata tattgctaaa gcttttatct catcgtttat tgccattacg gttggggcgg
    12841 gaattaccct ggcggtgctg attctctcag ggtcgattga atttatcccg atgcaggtga
    12901 tccctatcgc cgggatgatt gccggtaacg ccatggtagc ggtggggttg tgttacaaca
    12961 atttagggca acgggtcatt agcgaacagc aacagattca ggagaaactg agtcttggcg
    13021 cgacgccgaa gcaggcttca gcgatattga ttcgcgacag tattcgcgcg gctttaattc
    13081 cgacggtcga ttcagcaaaa acggttggct tagtgagttt accaggaatg atgtccgggc
    13141 tgatatttgc cgggattgat ccggtgaagg cgattaaata tcagattatg gtgaccttta
    13201 tgctgctctc aaccgccagc ttgtcgacca ttattgcctg ctatttaacc tatcgtaagt
    13261 tttataattc gcgccaccag ttggtggtga cgcaattgaa gaagaaatga tgcgatgccg
    13321 gatgcgcagc atcgcatccg gcgttgtggt tcatgtgccg aacaacggaa tatcaataca
    13381 acaatgcata cagctggcgg cgatacttcg acgccagtgc atcacccgta cccagcgcag
    13441 cgaggatctc ctggaacgtt ttacgcgtct gaccgtctgc ggcggtgaga tctttacgca
    13501 gatgcccgaa cagcaactcc agcgcctctt cattgcgccc aacctgatgc agttgcagcg
    13561 ccagttgcgt cgccagtgcg gcatcttctg gattctccgc cacctgctgt tgcaactgtt
    13621 gaatttccgg cgtatcagcc gcctgcttca gcagttcgat ttgcgccacc agcccctggt
    13681 agcgggtgtc ctgatcctgc aacggaatgg ttttcagcac cgcttccgca tcttcagaac
    13741 ggttcagcgc aatcagcgtt tctgccagca gcaggccgat ctccccgttc tgattcgaca
    13801 actgccaggc gtctttcagc aatggcaggg catcggtgta attgccttcc tgcatcagtt
    13861 gcatcgcctg ctgcgctttc agctcttctt cgcgcggcag cactttatcc agcagggcgc
    13921 ggatcgcctc ttccggttgc ggcccctgga agccatctac cggttgcccg ttctggaaca
    13981 gatacacggt cggaatcgcg cgcagaccaa actgggcggc aatcatctgc tccgcgtcgc
    14041 agtccagctt cgccagaata aattgcccgt tgtactgcgc cgcgaggctt tccaggatcg
    14101 gggttaactg caaacagtgc tggctacgtt cagaccaaaa atagaacagc accggagtgg
    14161 tcatcgactg ttcaagaacc tgttgcaggt tagattcgtt aatgttgaca atattttcta
    14221 cggacatgga gtcgctctct gttgtcgata ttttctttga catgggggct taagcgcgcg
    14281 cttcaactca cccctgcaat attttgtcca tcacgcgccc aggcagcagg cgcttgagca
    14341 ccattactgc ccaggtcacc agcgttaccg gatagcgcat cttcggcttc tcgctaataa
    14401 aagcatggcg cactttgtcg accaccgctt ccggtcccaa cgtaaagcgg gcggcgatgc
    14461 cgggattttc gactggttta tcactttgcg tctggttgac gttgtcggtg aagcgagtac
    14521 gaatgggacc gggttcgatc aggctgactt taattccgct gtggcgtagc tccatgcgca
    14581 gcgcgtctga ccacgcctcc agcgcatatt tactggccgc gtaagcgcca cgacccggcg
    14641 tggagattaa tcccatcacc gatgatgtca tcacaatacg cccttcaccg tgcggtaaca
    14701 tcgcgggtaa caggcgcatg gtgagctggt gtgcgccgaa aaagttggcg gaaaactgct
    14761 gttccatctg cgcacggctg atggtggaaa gggggccata catgccgaat ccggcattgt
    14821 taaagatccc atacagacaa ttatcggtca gggcgatcac ctcgtctgct gcgcgatcaa
    14881 cactttctgg tgaatccaga tcgatcaaca cgccggtaaa tcccatgttg ttcatacgct
    14941 caacatcatc cggttttcgg caacctgcca gcacatgaaa accctggcgt tttaattcga
    15001 gcgcgctttc caggccaatt ccactggaac atccggtaat taagaccgat ttttgcataa
    15061 ctttacctgt caggatctcc gttgctttat gagtcatgat ttactaaagg ctgcaactgc
    15121 ttcgccatcc agtcggcaat aaacggctgg gcgtcgcggt tgggatgaat accgtcatcc
    15181 tgcatccatt gtggcttgag gtagacctct tccataaaaa agggcagcag cggaacatca
    15241 aactctttgg cgagtttggg gtaaatggcg ctaaaggctt cattataacg gcgaccatag
    15301 tttgcaggca gacgtatttg cattaacaat ggttcagcgt tggcggcttt gacatcctgc
    15361 aaaatctggc gcagcgtttg ctcggtttgc tgtggctgaa aaccacgcaa accgtcattg
    15421 ccgcccagtt caaccagcac ccagcgcggc tgatgctgtt tcagcagagc cggaaggcgc
    15481 gccagtcctt gttgcgaggt gtcgccgctg atgctggcat taactaccga cgttttactc
    15541 tgccacttat cattcaacaa ggcaggccag gccgcgctgg cagacattcg atacccggcg
    15601 ctcaggctat cacccagaat caataacgtg tccgctgcgg cggcacggaa ggttaacagg
    15661 accaggaaca ggaagggcaa atgccagcgg aaaacattgt tgaagttcat catcttaaga
    15721 agtccgtcgg tcagggggag catgaactct ccatcctcac cggagttgag ctggttgtca
    15781 aacgtggcga gaccatcgca ctggtgggcg agtcgggatc gggtaagtca accttgctgg
    15841 cgatcctcgc cgggcttgat gacggcagca gtggcgaagt gagtctggtg ggacaaccgc
    15901 tacataatat ggacgaagaa gcgcgggcaa agttgcgcgc gaagcacgtc ggctttgttt
    15961 ttcagtcatt tatgttaatt cctaccctta acgcgctgga aaacgtcgag cttccggctc
    16021 tgctgcgcgg tgagagtagc gcggaaagtc gtaacggggc gaaagcgttg ctcgaacagt
    16081 tagggctggg taaacgtctg gatcatcttc cggcacagct ttccggcggt gaacagcaac
    16141 gagtggcgct ggcacgagcc tttaatggtc gacctgatgt gctgtttgcc gacgaaccca
    16201 ccggcaacct tgaccgccag acgggcgata aaattgccga cctgctgttt tccctcaacc
    16261 gtgaacatgg caccacgttg attatggtga cccacgacct gcaactggcg gcacgctgcg
    16321 accgctgctt acggctggtg aacgggcagt tgcaggagga agcatgattg cacgttggtt
    16381 ctggcgcgaa tggcgttcgc cgtcgctatt aattgtctgg ctggcgctaa gcctggcggt
    16441 ggcctgcgtg ctggcgctgg gcaatatcag cgatcgcatg gagaagggct taagccagca
    16501 aagccgtgag tttatggcgg gcgatcgggc gttgcgcagt tcacgcgaag tgccgcaagc
    16561 gtggctggag gaagcgcaaa agcgcggcct gaaagtcggc aagcagctga ctttcgccac
    16621 aatgaccttt gcaggcgaca caccgcagct ggcgaacgtc aaagcggtgg atgatatcta
    16681 cccgatgtat ggcgatctgc aaactaatcc ccctggcctg aaaccgcagg cgggcagcgt
    16741 attgctggcc ccacgcctga tggcactgct taacctgaaa acgggcgaca ccattgacgt
    16801 gggcgatgcc accttgcgga ttgccggaga agtgattcag gaaccggatt ccggttttaa
    16861 ccccttccag atggctccgc gtctgatgat gaatctggcg gatgtcgata aaaccggagc
    16921 cgtgcagccg gggagtcggg tcacctggcg ttataaattc ggcggcaacg agaaccagct
    16981 cgacggctat gagaaatggt tgttacctca gcttaaaccc gaacaacgct ggtacggtct
    17041 ggaacaggac gaaggcgcgc tggggcgatc gatggaacgc tcgcaacagt tcctgctgct
    17101 ttcggcgctt ctgaccttgc tgctggcagt ggcagcggtg gcggtagcga tgaatcatta
    17161 ctgtcgcagt cgctacgatc tggtggcgat cctcaaaacg ctgggggcag ggcgagcgca
    17221 actgcgtaag ctaatcgtcg gtcagtggtt gatggtgctg acgctttcag ccgttaccgg
    17281 tggggccata ggcctgttgt tcgaaaacgt gttgatggtg ctgctcaagc ccgttctgcc
    17341 tgctgcacta ccgccagcca gcctctggcc gtggctgtgg gcgcttggca ccatgacggt
    17401 catctcgctg ctggtggggc tacgaccata ccgcttgttg ctggcaacgc agcctttacg
    17461 cgtattacgt aatgatgtgg tagcgaacgt ctggccgctg aagttttatc tgccgattgt
    17521 cagtatggtg gttgtgctgc tgctcgccgg attaatgggt ggcagcatgc tgctttgggc
    17581 ggtgctggcg ggcgcggtag tactggcttt gctgtgcggt gtgctgggct ggatgcagct
    17641 gaatgtactt tgccgcatga cgctgaaatc gctacctctg cgcctggcgg ttagccgcct
    17701 gttacgtcag ccgtggtcaa cgttaagcca gctttcggca ttttcgctct cctttatgct
    17761 gctggcgctg ttgctggtat tgcgttgcga tctgctcgac cgctggcagc agctaccgcc
    17821 agaaagcccg aactactttt taattaacat cgccacagaa caggtagcac cgctaaaagc
    17881 gttcctcgcg gaacatcata tagtcccgga atcattttat ccggtagtgc gggcgcggct
    17941 gacggcgatt aacgataagc cgacagaagg taatgaagat gaggcgctta atcgcgaact
    18001 caatcttacc tggcaaaata cgcggcccga tcataatccg attgtcgccg gtaactggcc
    18061 gccaaaagcc gatgaagtgt cgatggaaga ggggctggcg aaacgcttaa acgttgccct
    18121 cggtggtacc gtgactttta tgggcgatac ccaggagttc cgcgctaaag tgaccagcct
    18181 gcgcaaagtg gactgggaaa gtctgcggcc taatttctat tttattttcc cggaaggggc
    18241 attagacggg caaccgcaga gctggcttac cagtttccgc tgggagaatg gcaacggcat
    18301 gttgacgcaa ctcaaccgcc agttcccgac cattagcctg ttagatattg gcgcgatttt
    18361 aaaacaggtc ggttaggtgc tggagcaggt aagtcgggcg ctggaggtga tggtggtgct
    18421 ggtcaccgcc tgcggtatgt tgctgttgct ggcacaggtg caggtgggaa tgcgccagcg
    18481 tcatcaggag ctggtggtat ggcgcacact cggtgcgggg aaaaaactgc tgcgcacgac
    18541 attgtggtgt gagttcgcca tgcttgggtt tgtttccggc ctggtggccg caattggtgc
    18601 ggaaacggca ctggcagtgt tgcaggcgaa agtgtttgat ttcccgtggg agccagactg
    18661 gcgattgtgg attgttctgc cgtgcagcgg tgcgctgctg ctgtcgcttt tcggcggctg
    18721 gctgggtgcg cgactggtta agggtaaggc gctgttcagg cagtttgcgg ggtgatgaga
    18781 gtaaataagg ggcgttctgg ttgaatggaa cgccttgttt aaattaattc tacaatatgt
    18841 ttcattgatg atgttattga attggtgatt tcctatcttt ctattgctga tattaatctg
    18901 aattggtgga gtttaataat tttaaatata gccctacaaa aataactcag actaataaaa
    18961 t
//
LOCUS       DAZPEB010000064        18701 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZPEB010000064 DAZPEB010000000
VERSION     DAZPEB010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18701
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.065"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..378
                     /locus_tag="ACHURJ_001962"
     CDS             <1..378
                     /locus_tag="ACHURJ_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1280923.1"
                     /translation="ICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDEKF
                     MNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSHRDFLNLKEIKLEHPLPSIKLYI
                     SYNKSSLNNLVFSRFIDRLNESF"
     gene            525..1508
                     /locus_tag="ACHURJ_001963"
                     /pseudo
     CDS             525..1508
                     /locus_tag="ACHURJ_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            1512..2042
                     /locus_tag="ACHURJ_001964"
     CDS             1512..2042
                     /locus_tag="ACHURJ_001964"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFR1280924.1"
                     /translation="MAIHQVSPFREEPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQ
                     KSIEIDGFTQPIVVTHTDKNAMEIVDGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRN
                     QRIAATIRHNRARGRHQITAMSEIVRELSQLGWDDNKIGKELGMDSDEVLRLKQINGL
                     QELFADRQYSRAWTVK"
     gene            complement(2043..3192)
                     /gene="ybdL"
                     /locus_tag="ACHURJ_001965"
                     /pseudo
     CDS             complement(2043..3192)
                     /gene="ybdL"
                     /locus_tag="ACHURJ_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308666.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
     gene            3301..4389
                     /gene="hcxA"
                     /locus_tag="ACHURJ_001966"
     CDS             3301..4389
                     /gene="hcxA"
                     /locus_tag="ACHURJ_001966"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFR1280925.1"
                     /translation="MPHNPIRVVVGPANYFSHPRSFNHLNDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4399..4596)
                     /locus_tag="ACHURJ_001967"
     CDS             complement(4399..4596)
                     /locus_tag="ACHURJ_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFR1280926.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4778..6883)
                     /gene="cstA"
                     /locus_tag="ACHURJ_001968"
     CDS             complement(4778..6883)
                     /gene="cstA"
                     /locus_tag="ACHURJ_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFR1280927.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPKRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(7064..7477)
                     /gene="entH"
                     /locus_tag="ACHURJ_001969"
     CDS             complement(7064..7477)
                     /gene="entH"
                     /locus_tag="ACHURJ_001969"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFR1280928.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTCDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7480..8226)
                     /gene="entA"
                     /locus_tag="ACHURJ_001970"
     CDS             complement(7480..8226)
                     /gene="entA"
                     /locus_tag="ACHURJ_001970"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFR1280929.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8226..9083)
                     /gene="entB"
                     /locus_tag="ACHURJ_001971"
     CDS             complement(8226..9083)
                     /gene="entB"
                     /locus_tag="ACHURJ_001971"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFR1280930.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFELQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPVPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(9097..10707)
                     /gene="entE"
                     /locus_tag="ACHURJ_001972"
     CDS             complement(9097..10707)
                     /gene="entE"
                     /locus_tag="ACHURJ_001972"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFR1280931.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASGSIAVID
                     GELQLSYRELNQAADNLACTLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLHNDSGE
                     HNLQDAINHPADGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLSQWLASRASA"
     gene            complement(10717..11892)
                     /gene="entC"
                     /locus_tag="ACHURJ_001973"
     CDS             complement(10717..11892)
                     /gene="entC"
                     /locus_tag="ACHURJ_001973"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFR1280932.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKVQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QASARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVLASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12267..13223
                     /gene="fepB"
                     /locus_tag="ACHURJ_001974"
     CDS             12267..13223
                     /gene="fepB"
                     /locus_tag="ACHURJ_001974"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFR1280933.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPTGLNASQSQGKRHDIIQLSGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(13227..14477)
                     /gene="entS"
                     /locus_tag="ACHURJ_001975"
     CDS             complement(13227..14477)
                     /gene="entS"
                     /locus_tag="ACHURJ_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFR1280934.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWLVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAEIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            14588..15592
                     /gene="fepD"
                     /locus_tag="ACHURJ_001976"
     CDS             14588..15592
                     /gene="fepD"
                     /locus_tag="ACHURJ_001976"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFR1280935.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15589..16581
                     /gene="fepG"
                     /locus_tag="ACHURJ_001977"
     CDS             15589..16581
                     /gene="fepG"
                     /locus_tag="ACHURJ_001977"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFR1280936.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16578..17393
                     /gene="fepC"
                     /locus_tag="ACHURJ_001978"
     CDS             16578..17393
                     /gene="fepC"
                     /locus_tag="ACHURJ_001978"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFR1280937.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIKHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTHWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLERTAPSTANS"
     gene            complement(17390..18523)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHURJ_001979"
     CDS             complement(17390..18523)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHURJ_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFR1280938.1"
                     /translation="MSSLNIKQGSDAHFSEYPLASPSSNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSSAVITPAEAIQWQDLEKTFTKLRVLDLDVNIDRGG
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSKEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
BASE COUNT         4445 a         5265 c         4766 g         4225 t
ORIGIN      
        1 atttgtagta ataagcatcc gcgaattact gacaatagca cttatgaaca aattatggca
       61 gaagagttca cccaattaat atccaaatca gcaggagttg atgatattca aatggaaata
      121 gacgagaagt ttatgaatcg caaaatttca tttcgtggtt cctctttgct gacaataatt
      181 aatagcattg cagttacaga tttactggga atagtcccct atgaacttta taactcccac
      241 cgtgactttc ttaatttaaa agagataaaa ctagaacatc cgttaccttc aataaagctt
      301 tacatttcct ataataaatc ttctttaaat aatttggtgt tctcccgatt tattgaccgt
      361 ctaaatgaga gtttttgata tataactaca gacagctact ataactttca tctatttatt
      421 cacagcgcga atgaaaaata aaacactctg ctttacagtg tttactttat aattaacagt
      481 cttatctcac tgttcaccct gtaacgttca gagagcgtct tcagatgtct atttataaaa
      541 ttcctcttcc gctcaatatt ctggaagctg cacgagagcg tattacctgg acactcaaca
      601 ccctaccccg cgtttgtgtc tcattttctg gcggcaaaga ctctggttta atgctgcatc
      661 tgacagccga acttgcccga caaatgggca aaaaaatctg cgttttgttt atcgactggg
      721 aggcgcaatt ctcttgcact attaactatg ttcagtccct gcgcgagttg tacaccgatg
      781 tcatcgaaga gttttactgg gttgcgctcc cgcttacgac gcaaaattcc ctttcacaat
      841 accaacccga atggcagtgc tgggaacctg atgtcgaatg ggtgcgtcag cccccgcaag
      901 atgcgataac cgatcctgac tttttctgct tttaccagcc aggcatgacc ttcgaacaat
      961 ttgtacgtga gtttgccgaa tggttttcac aaaaacgtcc ggcggcgatg atgatcggca
     1021 tccgtgcgga tgagtcctac aaccgttttg tcgccatcgc cagtttaaat aaacaacgtt
     1081 ttgccgacga taaaccatgg actacggccg caccaggcgg tcatagctgg tacatttacc
     1141 ccatttacga ctggaaagtg gctgatatct ggacctggta tgcaaatcat caaagtctct
     1201 gcaacccact gtataaccta atgtatcagg caggcgttcc tctgcgtcat atgcgaattt
     1261 gcgaaccttt tggcccggag caacgacaag gattatggct ctatcacgtt atcgaaccgg
     1321 atcgctgggc tgctatgtgc gcacgagtca gcggggtaaa aagtggcggc atttacgccg
     1381 gacatgacaa tcatttctat gggcatcgga aaatcctcaa gccagaacat ttagactggc
     1441 aagaatatgc attattgctg ctcaatagca tgccggaaga tgatcgcatc aaagcgatta
     1501 atgaaatccg catggcgatc catcaggtta gcccctttcg tgaagagcca gtggattgcg
     1561 ttctgtgggt taaaaacagc caacttatgc ctaatgatta caacccaaac aacgtggcac
     1621 cacccgaaaa aaaactgctg caaaaatcca ttgaaataga tggttttacc caacccattg
     1681 tggtcacaca tacggataaa aatgccatgg aaatcgtgga tggttttcac cgccatgaaa
     1741 ttggtaaagg ctcatcatca ttaaaattac gcttaaaagg ttatttgccg gtgacctgtc
     1801 tggaaggtac gcgtaatcaa cgcatcgccg cgactattcg tcataatcgc gctcgtggac
     1861 gccatcaaat caccgccatg tcggaaatcg tccgtgaact tagccagttg ggatgggacg
     1921 ataataaaat tggcaaagag ctgggaatgg acagcgacga agtgctgcgc ctgaagcaaa
     1981 ttaacggcct gcaagagttg tttgccgacc gtcaatattc ccgcgcctgg acggttaaat
     2041 agctaaagct ggcgcaggcg ttcagctgct gccagcaacg tcgattcctt cttggcaaaa
     2101 cagagacgaa tcagtttatg tgggaaggga tcggcgcaaa acaccgacag cggaatcgcc
     2161 gccacgccgt gctcccgcgt cagccactgg caaaactcaa catcatccag ggtagaaacc
     2221 tcgctgtaat ccaccagcaa aaagtatgta ccttcacacg gtaaaatctc cagccggctt
     2281 tcatttaaag cattcaccag aatatcgcgc ttctggcgat aaaagtccgg taacgcaaga
     2341 taatgctcag gttctgcacg tagcatatca gcaagcgcca gctgtgccgg ggtattcacc
     2401 gaaaaggtca gatactgatg taccttgcga atttcggcgc tgatgggcgc tggcgcaaca
     2461 caataaccca ctttccagcc ggtcatatga tagatcttgc caaatgaaga aaccgccact
     2521 gcccgctcac gcagctgcgg atgcgccagc acactggcat ggccctgttg tgaaaagttg
     2581 atgtgctcgt agacttcatc gctaatgaca aaaatctcgt gcccggcgat cgcctgccac
     2641 aaagcggcga aatcagcctg ctgccagaca gttgcactgg ggttatgcgg agtgttgagg
     2701 atcaccagtc tggtgcgctc gcttaataat gcggcaaatt cctgccagtc aaggtggttg
     2761 cagtgccata cgcttcacta ttcccccaga aagcgcgatg gcgggggcgt aactgtcata
     2821 gctgggatca aaacaaatca cttcatcgcc attgcgcacc agtgcggtaa tcgccgcgta
     2881 taacgcttcc gtcgcccctg ccgttacggt gatatcgcta tcggcatctg gttgatagcc
     2941 atacaaacgt tccgttttct gagcaatcgc ctcgcgcaag gcctgcacgc cggtcatggg
     3001 cgcgtattgg tttgccccct gggcaacgtg gtacgccagc cgctcctgta aatagcgcgg
     3061 accatcaaaa tcaggaaagc cttgcgacag gttaatcgcc tggtgttgct gcgccagcgc
     3121 gctcatctgg gtgaaaatag tggtgccaag ttgtggaagt ttgctttgtg gaatcagagg
     3181 gttatttgtc atttttattg tacctgacga agttgcgtgt tgaaggcact ataacacgat
     3241 gctagtattt ggcaatcaag acgtttagat gtctaaataa aacaataagg acaacacaac
     3301 atgcctcaca atcctatccg cgtggtcgtc ggcccggcta actacttttc acatccaaga
     3361 agtttcaatc acctgaacga ttttttcact gatgaacaac tttctcgcgc ggtgtggatc
     3421 tacggcaaac gcgccattgc tgcggcgcaa accaaacttc cgccagcgtt tggacttcca
     3481 ggggcaaagc atattttgtt tcgtggtcat tgcagcgaaa gcgatgtaca gcaactggcg
     3541 gctgagtccg gtgacgatcg cagcgtggtg attggcgtcg gcggcggcgc actgctcgac
     3601 accgcgaaag ccctcgcccg ccgtctcggt ctgccatttg ttgccgttcc gacgatcgcc
     3661 gctacctgcg ccgcctggac accgctctcc gtctggtata acgatgccgg acaggcgctg
     3721 cattatgaga ttttcgacga cgccaatttt atggtgctgg tggaaccgga gattatcctc
     3781 aatgcaccgc aacaatatct gctggcgggg atcggtgaca cgctggcgaa atggtatgaa
     3841 gcggtggtgc tggctccgca accagaaacg ttgccgctaa ccgtgcggct ggggatcaat
     3901 aatgcgcagg ccattcgcga cgtcttgtta aacagtagcg aacaggcact tgccgatcag
     3961 caaaatcagc agttaacgca atcattttgc gatgtggtgg atgctattat tgctggtggt
     4021 gggatggtcg gtggtctggg cgatcgtttt acgcgtgtgg cggcggctca tgccgtgcat
     4081 aacggtctga ctgtcctgcc gcaaaccgag aagtttcttc acggcaccaa agtcgcttac
     4141 ggaattctgg tgcaaagcgc cttgctgggt caggatgatg tgctggcgca attaaccggg
     4201 gcgtatcagc gttttcatct gccgactacg ctggcggagc tggaagtgga tatcaataat
     4261 caggcggaga tcgacaaagt gattgcccac accttgcgcc cggtggagtc cattcattac
     4321 ctgccagtca cgctgacacc agatacgttg cgtgcagcgt tcgaaaaagt ggaatcgttt
     4381 aaagcctgac gtgcaaattt agcagcagcg cgcgccgcct tttccaccgt agcgcgcgtc
     4441 ctgccgctca cggaaaaact cctcgtaggt catcggcgtt tgatcgggat ggttaacccg
     4501 catatgttcg acatagttgt cgtaatcagg cataccaatc atcagcttcg ccgcctgacc
     4561 taaatatttt ccggcttttg ccagtgaatc aaacatcttg gcctccgcta accatgttgc
     4621 tgaaagagtg ccggatgcgg cgtgaacgct ttatccggcc tacaaatgct atcaacgcac
     4681 tcggtcagtc ccctcgcccc atcggggaga gggttagggt gagggggaac cctcggcact
     4741 atgccaacat tcgccaacat cccccctcac tctgacttta gtgcgcgcct ttcgcctgcg
     4801 ctacgatctc ctcgacattt tccggcatcg gctcatacgg cgtttctttc gccgttggct
     4861 tcggatcttt caatgccgca agtgccgtct taatcgagaa cagtgccaga accaccacga
     4921 ccaccataaa gaagatggtt aacccggcat ccagacggtt gttgaacacc agttgcgcca
     4981 gctgtgactc agtatactgc gacggaatat tgccgctgtc gatcattgcc tggaacttgt
     5041 tagcaatggc caggaagccg actttcgcat ccgggctaaa cgctttctgc cagccagcgg
     5101 tcagggtaca aatcagcagc caggccgttg gtaccagcgc cacccaggcg taacgttgac
     5161 gtttcatctt gaacaacacc acggcacaga gcatcagcgc catccctgcc agcatctggt
     5221 tggcaatacc aaacagcggc cacagagtgt taataccgcc cagcggatcg accacgccct
     5281 gatggaggaa gtagccccac gccagtacgc acagcgccgt tgccagcagg ttagcaggca
     5341 gtgaatcggt ccgcttcagg ccaggagaca ccacgcccag cagatcctgc aacataaagc
     5401 gcgcagcacg cgtacccgca tctaccgccg tcagaataaa cagagcttca aacagaatgg
     5461 cgaagtgata ccagaacgcc acatccatca taccacccag cgcaccgtgc aggatgtaag
     5521 ccatccccac cgccaacgtc ggcgcaccgc ccgcacggga aatgatcgac tgttcaccca
     5581 cttcgctggc aatctggttg agcgtatctg gtgtaatgct aaagccccag ctactcacca
     5641 cctgcgcggc agaagcgacc acatccgccg tccctgctgg agccagcacc gccattgggc
     5701 tgttcatggc aaagtaaacg cccggatcga tgatacaggc ggaaaccagc gccataatcg
     5761 ccacgaagga ttccattaac attccaccgt agccgataaa acaagcctgc ccttcgttcg
     5821 ccagcatctt cggcgtggtg ccggaagaga tcagcgcatg gaagccagac accgcgccac
     5881 aggcgatggt gataaacagg aacgggaaca ggttaccggt ccataccggg ccagtgccat
     5941 cgacaaattt ggtcagcgca ggcatggtca gcgtcgggcg cataatcaaa atgcctaccg
     6001 ccagaccgac gatcgtcccg attttcagga aggtagagag gtagtcacgc ggggccagca
     6061 gcaaccacac cggcagcacc gccgccacaa aaccgtaacc caccagcatc caggtcagct
     6121 gcacgccggt aaagtcaaag tacggtgccc aggtcggact ttctgccacc cagccgccag
     6181 agataatggc gaaaatcagg aataccagac cgatgaccga cacttcacca atacgccccg
     6241 gacgcagata gcgcaggtag atccccataa acagcgccag cggaatggtg aacgcaacgg
     6301 tatacgttcc ccacgggcta tgagtcaggg ctttcaccac gatcatcgcc agtactgcaa
     6361 ggataatgac catgatcata aagcaggcca ccagcgctat caccccggcg gttggcccca
     6421 tctcttcttt gaccagctca cccagcgatc gaccgtcacg gcgcgtagaa acaaacagca
     6481 ccatgaaatc ctgcaccgca ccggcgagaa ccaccccggc gagtagccag atcatccccg
     6541 gcaggtagcc catttgcgct gccagcaccg gcccaaccaa cggacctgct ccggcgatgg
     6601 ccgcaaaatg gtgaccgaac agcactttct tgtccgtcgg cacatagtcc agcccgtcgt
     6661 tatggcgcac cgctggcgtc atacgcttcg ggtcaaccgc cagcacattt ttggcgatat
     6721 acaacccata aaaacggtaa gcgatcagat agatacagac cgacgccacc acaatccaca
     6781 gcgcgttgat ctgttcccca cgatttaaag caatgtatcc cagagcaaat gctcccatta
     6841 cagagagcac tgtccagacg aggtatttcc ctgatttgtt catagttgtt atccgtgtgc
     6901 gtgtccatag agatgttaca ttttgtttct ataacatctt tgtacgattt aacaacacgg
     6961 taactatgta aatcggagtt gaaaccaata tttaacttaa caatgttaac tcgatcactc
     7021 cgttaaccga gttttcctgc aatctcattt aattctgtcg ggctcatccc aaaactgccg
     7081 tacccagccg acaagtgcag caacgccgcc cctgttcatc gaaaacgacg atttcccagc
     7141 tctgattttg ccgcccaaga tgcagcggct ggcagacgcc gcgtaccttg ccttcagaca
     7201 ccgggcgatg atgggtggcg ttaagttccg tgcccaccac acactgcccg tcacaggtca
     7261 tcataaatcc ggccatcgat cccagcgttt cagccagcgc cgccgacgcg ccgccatgca
     7321 gcaggccaaa cggctgatga gtacgggtat caaccggcat ttcggcttcc agcacatcat
     7381 ctcccagacg ggtatacaca attcccagat gcgccaccat tgtgttatcg ctggtggcgt
     7441 tcagttcgtc gagcgttaaa tggcgtttcc agatcatgct tatgccccca gcgttgagcc
     7501 gccatcgacc acaatatcct gtagggtgat atggctggcg aggtcagagg cgaggaacaa
     7561 aatcgtgttg gcgatctctt gtggacgggc gattttcccc agcggaatgc cgagtttaaa
     7621 ctgctcgcca aagccgcgaa tacgctgttc ttcggcgtca tcgctcaccc acagcgtgcg
     7681 ttgcatatcg gtgtcggtgg agccaggcga aaccacatta cagcgcacgc cgctacccgc
     7741 cagttccagc ccgacgctca acgccaggct tttcagcgcc gctttcgatg cgccataagc
     7801 actcatacca atacgcggcg tgtgtgcggc gtcggacgcc acagtgacaa tcgccccgcc
     7861 ccgctgacgg cgaaactggt tcatggtttg ctggaacagg ttaaacgcac cgccgacgtt
     7921 aaccgcaaaa gtctgctgcc agtcctcttt actgagctga tcggtcgcgc ccatgcgcaa
     7981 aattcccgcc gcattgatca gcacgtccag tcgctccgtt tcagctaaca gtcgctgaca
     8041 tacctgcgcc acctgcccgg cgtcggcaac atccattact tcggtcgcaa agggatattg
     8101 ctcctgagtg aacgcttgat caaaacctgt aactttcgct cccgcctcaa caaacgccag
     8161 cgccgtggcg tagccgatac ctttacctgc gccggttacc cagacatttt taccgctgaa
     8221 atccattatt taacctcgcg ggagagtagc ttccaccagg cgtcgatggt cgggtttttc
     8281 gccagcatga caaagtcgat atcaccatgc actttgcgcc agcgcgccgc cagcgccatc
     8341 atgcgcaccg aatccagacc gtagtcgatc aggttgtcgt catcgaacgg ttcatcggac
     8401 tcgtccagca acggcaggat aacctcacgc agcgccgctt tgctggcggg gacaggtgct
     8461 ggcagtaatt cttcagtcat caccacccgg ccagaccgcc cggccacata tttcagcgac
     8521 atcaaatgct cgtcacggct gaaatcggcc agcgcatccg ccaccataaa cggtttaata
     8581 tcgcgcataa atgcgtcggt tgcggtggtc atacagccaa tgtgggcata taccccggta
     8641 ataatcagct ggttacgtcc actctctttg agcatttgct ccagcggaga acgatgaaac
     8701 gcgctgtagc gccacttcac cagcaccgtg tcgtcggcat ctggcgtcag gcgatccacc
     8761 accttttgct gttccggcga acgggtcagg cccggccccc acatatcatt caacagcgcc
     8821 cgatcttcat cgctctgctc tttcggctgg gcggtgtaat aaactgggat attgtgctgt
     8881 ttgcagtagt cgcgcagcgc agcaatattc gcgatcacct gctccatcat cgggcagttc
     8941 tcgccccaga agctgacaaa atagtcctgc atatcatgga ttaacaacgc ggcacgttgc
     9001 agttcaaagg cccagtcaac tttattctgc ggaatatcgt gagactccgg cagtgcgtaa
     9061 gcctgtaatt ttggaatagc catcgtgttc tctccttcag gctgatgcgc gtgacgccag
     9121 ccactgactt aattgttttt tatcgacttt cccgaccgcc gtcagcggaa gtgaatctac
     9181 acactccacg cgatccggta atttaaattc ggcaataccc tgttcacgca ggaaacgacg
     9241 cacctgcacc gcgcgcagcg gctcttttac caccagataa gcacagcttt tttcgcccat
     9301 cagctcatct tccatgctca ccagtgcggc atagatcacc gccggatggc gcagcagcag
     9361 gttttcgatc tcttcggcag cgatcttctc gccgccacgg ttgatctgat ctttctcgcg
     9421 cccctgcacg gtgatgtaac cctctggatc aatagagatc agatcgccgg aacagtaaaa
     9481 accgttggca tcaaaggcgc tggcattgtg ctgcggactt ttgtaataac cgcggaaggt
     9541 gtacggcccg cgcgtcatca ggcgtccgac ttccccttgc ggcagtggat ttccttcggc
     9601 atcggcaacc cacacttcat cgtccggaca cattgggtaa ccctgggtat ggataatttt
     9661 ctccgcgcta tcatcaagac gggtgtagtt caccagccct tccgccatgc caaacacctg
     9721 ctgcaactgg cagccaatct cagcgggaat acgcgccgca agggtggcag aaagacgtgc
     9781 gccgccgacc tgtaacagtt tcagcgaggc aagctgcgcc cggctttcgc cttcagccag
     9841 cgcctgcaac cacaggctga ctgccggcgg caccagcgcg gtgacgttca cctgatgttt
     9901 ttcaatcaat gggaagcaaa gcgtagcgct gggatcggcc gccagaacaa ccgttcctcc
     9961 ggcaagaaag acgcccagcg atcctggcga actcatggcg tagttatgag ccgccgggat
    10021 cgcgcacagg tagcgcgtct gttgtgtgaa ctgacaaatc tcgacgctac gacgcacgct
    10081 gtagtagtag tcgttatgag tgcgcgggat cagcttcggt gtaccggtgg tgccgccgga
    10141 aagctggaaa taggccactt catcagcagg tgatggcgtg gcagtaaaac cgtctgccgg
    10201 atggttaatc gcatcctgca agttatgctc accgctgtcg ttgtgcagtt gcaccacgcg
    10261 aatggaggaa tgttctgtga cgaaagtatt gaggaaatca tccccgctaa acagcgcatg
    10321 ttggcgatcg gcaatcagca atgcgggttc aatctggctg gcataggcgt tcagttcact
    10381 acgctgatgg ctgaacaacg ccagcaccgg cgcaacgccc agtttcagca gcgcgaaaaa
    10441 ggtaatatac aattcagcga cgttacccag ttgtaccagc gcggtttcac caggtttaat
    10501 gccctgacgg cgtaaagtac acgcgaggtt atccgccgcc tgattcagct cccggtaact
    10561 caactgtagc tcgccgtcga taaccgcgat gctgccactc gcagcgtggc gagtcagaat
    10621 gtcggtcagc ggcaaatcct gccagtagcc tttttcccga tagcgacggg caaactcttc
    10681 cggccagcgg gtgaatggaa tgctcatcct cgctccttaa tgcaatccaa aaacattcaa
    10741 catggtagaa agtttgacgc ctgtttcgcg ccactcaccc aacggtgacg acgcaagcac
    10801 aatccccgct ccggcaaaca gacgcacctg attttcccgc agcttcgcgc agcggatggt
    10861 caccacccat tcgccgttac cttcgctgtc acaccaaccc acaatgccgc caaacagttc
    10921 gcggtcgaac ggctccagtt cagcaataac ctgagtcgcg gcctgatgcg ggaaaccgct
    10981 cagcgcgggg gtcggatgca gcagacaggc cagagtcagt gcgttttctt gcgaattcgc
    11041 tttaccttca aagggagttg cgagatgcca cagcgtcggc gtggtaatca attgtggaga
    11101 ggagggaacg tgtaactcac tactgcgttc gcgcagtacc tctttcatcg cctgagtcac
    11161 cagttcatgt tcatggcgat ctttttctga cgccagcaga cgattacccg cttcgcgatc
    11221 gagcacttca tccggctgac gacgcgcgga accggctaac ggaatggagc taaaacgctc
    11281 gccgtcttta cgtagcagca gttccgggct ggcccccagc aggacgccac catcagccag
    11341 cgggacatgg aagttgtaac taaccgggtt ttgcgcaatc aaccgttcca gcaatacgcc
    11401 actatcaatg gcggcgtcag tggtgatatc aatcaaccgt gacaacacca ctttgtcaac
    11461 ctgcggcgtg gcggtaagtg cggcagcgcg ggcaaccatc tgttcaaacg tggtttgttc
    11521 cggaattgcc tggcgttcca ccacattcag cgactggctg cgggtgaaac ggcgggcgga
    11581 agcttgtttt tcctgacggg agaacgactg ccaggattca ggaatataca gcgacgaagg
    11641 ctgacgtgga tcgaagggaa tcgccccgac catcaccgga tttttgatgc cctgcacttt
    11701 ggcatcggca aacagcgcgg cgagtttttg ctggaaggga ctgtcgggcg aatccccgtt
    11761 cacagccggt tcatcgaagc gggcgaaaca tcctgacgtc gtaaaactgc ggtacggcga
    11821 cataaagaaa aagcgattgg gcgcaagtgt tgccatggtc tgctgtactt cctcagccag
    11881 tgacgtatcc atatcatcct ccacaaaatg ataaaggctt taataatgat tatcatttat
    11941 attttcggtc gctaacctaa aagcaaacgc gcactatgtc aactcttgag gtaacgcaat
    12001 ctgcaactcg gggttgcatc tggttgtggt tacaatgaaa atgagaagca ttattgatgg
    12061 attcgcataa gcgcaatgtg atgtcctgcg ccgttcttcc ccctctccct tccagggaga
    12121 gggctggggt gagggtcaat attcgcacca gtgctggctg ctcccatcac cctaaccctc
    12181 tccccaaagg ggcgagggta cggatagtgc gctttgtcga atttgtcatt acgcccttaa
    12241 ccttattaat aacaggaagc tgatttgtga gactcgcccc gctctaccgc aacgcccttc
    12301 tattaacagg acttttgctt tccggaatag ccgcagttca ggccgccgac tggccgcgtc
    12361 agattactga cagccggggc actcataccc tggaaagcca gccgcagcgt attgtttcca
    12421 ccagcgtcac cctgaccggc tcactgctgg cgattgatgc tccggtgatt gccagcggcg
    12481 cgaccacgcc aaataaccgc gtcgcggatg accagggctt tttacgccag tggagcaagg
    12541 tggcgaaaga acgcaaacta caacggctct atatcggcga accgagcgcc gaagccgttg
    12601 ccgcgcaaat gccggatctg attttaatta gcgcaaccgg cggggattcg gcgctggcgc
    12661 tgtatgatca gctttccacc atcgccccga cattaatcat caattacgac gacaaaagct
    12721 ggcagtcgct gttaacgcag cttggcgaaa ttaccgggca tgagaaacaa gcggcagagc
    12781 ggattgcaca gtttgataag caactggcag cggcgaaaga gcaaatcaaa ttaccgccgc
    12841 agccggtcac tgctattgtc tataccgccg ctgcacacag tgccaatctc tggacgccgg
    12901 aatcagcaca agggcagatg ctggaacaac tcggctttac gctggcgaaa ttgcccacag
    12961 ggttaaacgc cagccaaagc cagggcaaac gccatgacat cattcagctt agcggagaaa
    13021 atctggctgc agggttaaat ggcgagtcac tattcctgtt cgccggtgat cagaaagacg
    13081 ccgatgcgat ttatgccaat ccactgctcg cgcacctgcc cgcagtacaa aacaagcagg
    13141 tttatgcgct gggaaccgag acgttccgcc tggattacta cagcgccatg caagtgctgg
    13201 aacgacttaa ggcgctgttt taagcattaa ctgtcggacg ctgtcacctg cggcggcgtc
    13261 tggcgaaaac gtcgcaactc caccagcacc agcaataaca acacgccgat aatcaacaaa
    13321 ccaaaaccgc tcgcgcttgc ggaagcaacc ggtgtcatca tcgctcccag accgcctaac
    13381 agcgccgcgc ctatggcatc gcccgtcacg ttttgtgccg tccacaaacc gttgattcgc
    13441 cctaacattg cttccggcgt ttgcgtttgc agcattgtat attgcagcaa cgaactgacc
    13501 gcactcagcc agccgaacag cgccagacaa accacgccta aaatccacat cggcatcagg
    13561 ccaaataaac caatggcgag gaacgatccc aacgtggaga gcaacatcaa caatcctggt
    13621 cgcgcgctat gcgccagttt cccactggtt aatgcgccaa tagccgcacc gagcgggatc
    13681 gccgcataga gaaaaccaat ctctgccgcc gacatctgcc agttgtcagc cagcgccgga
    13741 tacagtaccc gcaccgcgct cgccatcgtc aataaaccac ccagcaacgc aatcccgccc
    13801 accagcgggc tggcgagcag aaaacgaaat cctgccagta atgatttcaa cggatgctcg
    13861 cgcggctgcg gtggcggtgg caacgccgga aggcttaaca acggtagcaa ggtaataaac
    13921 gtgcccgccg ccgccagccc gtagttccag gctacgccac cggtcgccag caataaaccg
    13981 ccaatcatgg gcgaaatcac cgaccccaga cgcacggtca acatggtgat cgccccggcc
    14041 tgcattaagt tttcacgccc taccagtgct ggtgtcgccg ccaatagcgc cgtaacgcca
    14101 agcgatgcga aaaaaccatc ccataaacca agtaaataga ttgccagcaa tgacggctcc
    14161 ggcagcagtg cattaaggca cagtccaatg aagccaatgc cacaggtgcc gcgcgccagc
    14221 aaaatcactt ttttgcgctc atagcgatcc gccagcacac cgccaaccat caggccaaca
    14281 aacatcgcgc cgccggtcag cgtcaccgaa agccccacca gccaggtaga atgcgtcatc
    14341 atctggatct gcaccggcac cgcgacgccg agcaaaccca gagacacaat tgagataaaa
    14401 cgagcgagga atactgcgcg aaacgccggg tgcgttttca acaggctgag gttaagcagc
    14461 caggattgtt tattcattac aatgccttgc catcaaatat tttaaatacc tattcttagg
    14521 ctgcacatgc taacatatcc aaataagatc gataacgata attaatttca ttatcatgga
    14581 agttcgtatg tctggttctg ttgccgtgac acgcgccatt gccgtgcccg gattgctgtt
    14641 attactgatt atcgcgacgg cattaagcct gctcattggg gcaaaatcac tccccgcttc
    14701 cgtagtgctg gaggccttct ccggcacctg ccagagcgcc gactgcacca tcgtgctcga
    14761 cgcgcgactg ccgcgcaccc ttgccggttt actggcaggc ggcgcgcttg gccttgccgg
    14821 ggcgttgatg caaaccctca cccgaaatcc acttgccgac cccggcttgc ttggcgtgaa
    14881 cgccggagcc agctttgcca ttgtgctggg tgcggcgctg tttggttact catccgcgca
    14941 ggaacaactg gcgatggcct tcgccggggc gctggtggcc tcattgattg ttgcctttac
    15001 cggcagtcag ggcggcgggc agttaagtcc ggtgcgttta accctggcgg gcgtggcgct
    15061 ggcggcagtg ctggaagggc tgaccagcgg catcgccctg cttaatcctg acgtctacga
    15121 tcaactgcgt ttctggcaag ccggttcgct ggatattcgc aatctacata ccttaaaagt
    15181 ggtgctgatc ccggtgctga tcgccggagc aaccgcgcta ttactgagcc gcgcgctgaa
    15241 cagtttaagc ctcggcagcg acaccgcgac ggcgctgggc agtcgcgtgg cgcgcacaca
    15301 gttaattggt ctgctggcga ttaccgtgct ttgtggtagt gcgacagcgg tagttggccc
    15361 gattgccttt attggcctga tgatgccgca tatggcgcgt tggctggtgg gtgccgatca
    15421 tcgctggtcg ctgcccgtaa cgctgcttgc tacccctgcc ctgctgctgt ttgccgatat
    15481 catcgggcgg gtgattgtgc ccggtgaact gcgcgtttct gtggtcagtg cctttattgg
    15541 tgcaccggtg ctgatcttcc tcgtgcgacg taaaacgcga ggtggtgcat gatttacgtc
    15601 tctcgccgat tactcatcac ctgtttgctg cttatttccg cctgtgtggt tgcaggtatc
    15661 tggggattac gcagcggtgc cgtcacgctg gaaacctcgc aggtattcgc cgcgctgatg
    15721 ggcgatgcgc cgcgcagtat gacgatggtg gtcaccgaat ggcgtttacc acgcgtgctg
    15781 atggcgctgt tgattggcgc ggcactgggc gtcagcggtg cgatttttca gtcgctgatg
    15841 cgtaacccgc tcggcagccc tgacgtaatg ggctttaaca ccggggcgtg gagcggcgtg
    15901 ctggtggcga tggtgctgtt tggtcaggac ctgacggcta tcgcgctggc agcaatggtg
    15961 ggcggcattg tcacttctct gttggtctgg ctgctcgcct ggcgtaacgg tatcgacacc
    16021 tttcggttga ttattatcgg catcggcgtt cgcgccatgc tggtggcctt taatacttgg
    16081 ctgttgctga aagcgtcttt agaaacggcg ctaacagcgg gtttgtggaa tgccggatcg
    16141 ctcaatggcc tgacgtgggc aaaaacttcg ccttccgcac ccatcattat attgatgctc
    16201 attgccgccg ccttgctggt gcggcgaatg cgcttgctgg agatgggcga tgataccgcc
    16261 tgcgcgctgg gcgtcagcgt cgagcgttcg cgtctgttaa tgatgctggt tgcagtggtg
    16321 cttaccgctg cggcaacggc gcttgccggg ccgatttcct ttattgcttt agtcgcgccg
    16381 cacattgccc gacgcattag cggcaccgct cgctgggggc tgacccaggc ggcgctgtgc
    16441 ggagcgctgt tactgctggc ggccgatctc tgcgcccaac aactgtttat gccgtatcaa
    16501 cttccggttg gcgtcgttac cgtcagcctc ggcggtattt accttatcgt cttgttaatt
    16561 caggagtctc gcaaaaaatg accgaatcag tagcccgttt gcgcggcgaa cagttaaccc
    16621 tgggatatgg caaatatacc gttgcggaaa atctgactgt agaaattcct gatggtcact
    16681 tcacggcaat tatcggacca aatggctgtg gtaaatccac gttactgcgt accttaagcc
    16741 gcctgatgac gcctgctcac gggcatgtct ggctggatgg cgagcacatt aaacattacg
    16801 ccagtaaaga agttgcacgc cggattggtc tgttggcgca aaatgctacc acgccgggcg
    16861 atatcaccgt gcaggagctg gtggcgcgtg gacgttatcc gcatcaaccg ctgtttaccc
    16921 actggcgcaa agaggatgaa gaagcggtaa cgaaagcgat gcaggccacg ggaataactc
    16981 atctggcaga tcaaagcgtg gatacccttt ctggcggaca acgccagcga gcgtggatcg
    17041 cgatggtgct ggcccaggaa acggcaatta tgctgctcga cgaacccacg acctggctgg
    17101 atatcagtca tcagattgat ctactggaac tgttaagcga actgaaccgc gagaaaggct
    17161 ataccctggc ggcggtactg cacgatctta atcaggcctg tcgttacgcc agccatttga
    17221 ttgcattgcg ggaagggaaa attgttgctc agggagcgcc gaaggagatt gtcactgctg
    17281 aactgattga gcgcatttat ggtctgcgat gcatgatcat tgacgatccg gtagccggaa
    17341 cgccgcttgt ggtgccgctc gaacgaacgg caccttcaac cgcaaatagt taaactaagt
    17401 ggtctgccat catggcatcc tgttttctgg atgccatcgc atagcgcaat aacacgctac
    17461 cacaagccac catcccgccg atcagcgcgg aaaggatcac aataatcgcc ttacccggac
    17521 cgtctttttt cactggcaat gacgggctta actgatattt aaacggcgta aaattcacat
    17581 cgttgacatt tgcttttgtt aattgctcga caagatactg ccgattacgt aattcaccgt
    17641 tcagttccgc aacgtcagta accgcttttt caatttccag tttgcgttca ataccgtctg
    17701 cgccgagaga aatagagaaa tcggggtcat ctttaacggc ctgaccatta ctgtatacgg
    17761 gctttttaat tcctgccgcg ttggcaatgt cgagtgaata attgaggcgc tgaatgtttg
    17821 catcaagttg atttttcgtt ttaatgcgat cctgagccag tttttctttt tcaaactggg
    17881 ttttgatctc cagtttatta cggacgtttt ctatcgactc tttcaccacc aacgcagaga
    17941 tataatcgat atacccgctc aaaacggtct gcgcctcttt actggttggc gcggtaaaac
    18001 ttagcgtcca ggaggtatac agtgacggtt catctttttt cttactggca ttgtcatcaa
    18061 ccgctttcat tttttcgctc aatgcaacaa tagcgcgatg caaatccagt tcatcgattt
    18121 tcgcctcttt taattggtcc atcacataag gtgatgaacg caggtactct tccagcaagc
    18181 taacagactg aaacttcttg ataaacaggt taaatgcacc gccacggtcg atattaacat
    18241 cgagatcaag aacgcggagt ttggtaaagg ttttctccag gtcctgccac tgaatggctt
    18301 cggcgggagt aataacggct gagctggtcc atttttgcgg caggatgaaa gagatcagta
    18361 aacctgcgca ggcaaatgca aaaacgaccg ccatgaccgt ttttttggcc cgccataaaa
    18421 cttcgattag attaagtaaa tcaatttcat tactactggg cgacgccaga ggatactcgg
    18481 aaaaatgagc gtcacttccc tgtttaatat tcagtgatga catgggtata acctgaaaat
    18541 gagtccaata gggggaaatc tccacggcgc tcactttacc agttggtgag aattatccga
    18601 agctgaagtt tgtaaattcc ttccactgaa tgcaacattc actctattta agagaaatat
    18661 tcatcaaacc tataaatcaa acaaataaaa aacccataat t
//
LOCUS       DAZPEB010000065        17871 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZPEB010000065 DAZPEB010000000
VERSION     DAZPEB010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17871
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.066"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            1..682
                     /gene="csgG"
                     /locus_tag="ACHURJ_001980"
                     /pseudo
     CDS             1..682
                     /gene="csgG"
                     /locus_tag="ACHURJ_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(746..1237)
                     /locus_tag="ACHURJ_001981"
     CDS             complement(746..1237)
                     /locus_tag="ACHURJ_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFR1280939.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(1339..1893)
                     /gene="ycdY"
                     /locus_tag="ACHURJ_001982"
     CDS             complement(1339..1893)
                     /gene="ycdY"
                     /locus_tag="ACHURJ_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFR1280940.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWMEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(1917..2654)
                     /gene="ycdX"
                     /locus_tag="ACHURJ_001983"
     CDS             complement(1917..2654)
                     /gene="ycdX"
                     /locus_tag="ACHURJ_001983"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFR1280941.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(2709..3647)
                     /gene="ghrA"
                     /locus_tag="ACHURJ_001984"
     CDS             complement(2709..3647)
                     /gene="ghrA"
                     /locus_tag="ACHURJ_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFR1280942.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHWEDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            3881..3968
                     /locus_tag="ACHURJ_001985"
     tRNA            3881..3968
                     /locus_tag="ACHURJ_001985"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3915..3917,aa:Ser,seq:gga)
     gene            4013..4149
                     /locus_tag="ACHURJ_001986"
     ncRNA           4013..4149
                     /ncRNA_class="other"
                     /locus_tag="ACHURJ_001986"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(4240..4926)
                     /locus_tag="ACHURJ_001987"
     CDS             complement(4240..4926)
                     /locus_tag="ACHURJ_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFR1280943.1"
                     /translation="MSFMAFVTCLTIAFIAGFAVARKLTLRKVASSVIIAKSQPVPSS
                     ATSPAAKASTRSYIPQKTYRDSRWQERDELFQGMVAEGILLERKEWLITRCEKMYFDN
                     LEKWFGHYCHIHCQVSLGRLINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKI
                     VCVIELDDPSHERADRIIRDRWLDTIMTLARVPFVRASVTKLEEIPPVWETRDKIRPL
                     VAATKSEPVH"
     gene            complement(5507..6295)
                     /gene="phoH"
                     /locus_tag="ACHURJ_001988"
     CDS             complement(5507..6295)
                     /gene="phoH"
                     /locus_tag="ACHURJ_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFR1280944.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(6917..8188)
                     /gene="efeB"
                     /locus_tag="ACHURJ_001989"
     CDS             complement(6917..8188)
                     /gene="efeB"
                     /locus_tag="ACHURJ_001989"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFR1280945.1"
                     /translation="MQYKDENGVNEPSRRRLLKVIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFSLPGVKDANDYLGSALLRV"
     gene            complement(8194..9321)
                     /gene="efeO"
                     /locus_tag="ACHURJ_001990"
     CDS             complement(8194..9321)
                     /gene="efeO"
                     /locus_tag="ACHURJ_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFR1280946.1"
                     /translation="MTINFCRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITAQAEDLAQLRGVLGLD"
     gene            complement(9379..10207)
                     /gene="efeU"
                     /locus_tag="ACHURJ_001991"
                     /pseudo
     CDS             complement(9379..10207)
                     /gene="efeU"
                     /locus_tag="ACHURJ_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
     gene            complement(10876..12384)
                     /gene="putP"
                     /locus_tag="ACHURJ_001992"
     CDS             complement(10876..12384)
                     /gene="putP"
                     /locus_tag="ACHURJ_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFR1280947.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQES"
     gene            complement(12543..12752)
                     /locus_tag="ACHURJ_001993"
     CDS             complement(12543..12752)
                     /locus_tag="ACHURJ_001993"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1280948.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            12807..16769
                     /gene="putA"
                     /locus_tag="ACHURJ_001994"
     CDS             12807..16769
                     /gene="putA"
                     /locus_tag="ACHURJ_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFR1280949.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNCNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLENLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLVDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTYETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQCNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELHKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGIQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(16809..17447)
                     /gene="rutR"
                     /locus_tag="ACHURJ_001995"
     CDS             complement(16809..17447)
                     /gene="rutR"
                     /locus_tag="ACHURJ_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFR1280950.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFIIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            17735..>17871
                     /locus_tag="ACHURJ_001996"
     CDS             17735..>17871
                     /locus_tag="ACHURJ_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFR1280951.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     AL"
BASE COUNT         4488 a         4821 c         4380 g         4182 t
ORIGIN      
        1 ttcggtatac aacattcagg acgaaaccgg gcaatttaaa ccctacccgg caagtaactt
       61 ctccactgct gttccgcaaa gcgccacggc aatgctggtc acggcattga aagattctcg
      121 ctggtttata ccgctggagc gctagggctt acaaaacctg cttaacgagc gcaagattat
      181 tcgtgcggca caagaaaacg gcacggttgc cattaataac cgaatcccgc tgcaatcttt
      241 aacggcggca aatattatgg ttgaaggttc gattatcggt tatgaaagca acgttaaatc
      301 gggcggtgtt ggggcaagat attttgggat cggtgcagac acgcaatacc agctcgatca
      361 gattgccgtg aacctgcgtg tcgtcaatgt gagtaccggt gagatcctct cctcggtgaa
      421 caccagtaag acgatacttt cctatgaagt tcaggccggg gttttccgct ttattgacta
      481 ccagcgcttg ctggaagggg aagtgggtta cacctcaaac gaacccgtta tgctgtgtct
      541 gatgtcggct atcgaaacag gggtcatttt cctgattaat gatggtatcg accgtggtct
      601 gtgggatttg caaaataaag cagaacggca gaatgacatt ctggtgaaat accgccatat
      661 gtcggttcca ccggaatcct gacagataaa aaagccggaa ttatcatccg gcattttact
      721 gtttaagcga aagcgtgagt gcccctcacg ctttattttt gatttcctgc tcacggtgag
      781 ccgtctttgc actacgcgcc gccagccaca ggccgctgtt tttcattgcg taaccaaata
      841 ccagcccaag cgccagcgaa ggtaacacca gtttccagtc accctgcccg gcaaacgtcg
      901 cgcatgcgcc tataaacgtc cccggaacaa atgaaagcag cagctgtttg gcctgaatac
      961 acatcagaaa cgccacaata ccggttatga cataaccgag aatttccaga tgtggtgcca
     1021 gtgcactacc gtaaataatg accatcgccc acaccacgcc actaagcagg gttgcagcgg
     1081 agatcgccag ccctttcagc ccaccttgcg ggcaggcaaa gtaggccgta cagcccagga
     1141 agcccgccca gcttagtaag cccagggaaa cagccaccca tccccagata ccggagagaa
     1201 tgcctgttgt gattgcaata gagagaagta tgttcatgcc gcgcatgata gcagaaaacg
     1261 cggccatgaa tgagatcgag cacacatttt atgcaacaac aaattatctg ttgcagttat
     1321 gagtgacgat gatcacattt actcttcaga atcttcttcc agctcgtccc acattgcact
     1381 aatggcatca cgggttagcg gtgccatggt gcgccagaaa ggcgtggttg catgggcctc
     1441 cactttgccg aggaacgcac cacaccaggg taacagatac tcactgaaca gtgtttccag
     1501 tgcttcgctc tcatcttccg ttgactgatc ttccatccag gaagccgcaa gcagcaatgt
     1561 gccgatgtga tcggctggcg tatccgccag tggcatccct cgttcggaaa gaaaagcgcg
     1621 cacttccgct tccgttgcgt cctcaaccca tgcgctacga tatggcggca cggcacattc
     1681 atcgccgata aacaacgcat tgtaatcggc agagacttgc gccatatcac agcttttctg
     1741 taaacgcgtc agtaactcat cctgctccag tggccagttc gcagccagtt tcccctcacg
     1801 aatcagggta aacagcggca ccagtaaagg atcttgcggt tggcggtaat agagcgaacc
     1861 cagcacacga cagaggatag aaaactcgtt cattaatata tttccattac tagtaattaa
     1921 aggtctgcaa attccgcaat cggtgccata ccgcgagatt caaggaagtt cagtaagcgg
     1981 cgcggagaaa cattcaaaat gcgctctggc ggaaaatcta ccgcgtcgag gattttaaga
     2041 cactcttcaa attcccccat ggtaaacgcg gtgtgagaat ccgagcctaa tgccacccaa
     2101 cctccagcat cacgtaccgc tgcggctacc gcacgacagt tgtcttcact gcccttacgt
     2161 gagtgtaaaa atgaagaatt attgatttcc agcgccacct gatgtttcgc ggctgcttca
     2221 gcaacggctt tcacatctat ttcatatttg ggatttccgg gatggcttat tatatgcaca
     2281 ttgccgctgg cgatagtggc gatcatcgct tgtgtattgg tcgctttgtc atgtggtgca
     2341 aaaaccggct catgaaaacc ggcaataatc agatccagcg agtcaaacat tttaccgctg
     2401 caatcaattt caccatcaac atttttaatg ttagcttcga tgccgcgcag gatccctacc
     2461 ccatcaacca ctcgcggcca gatacgcatg ttaatgaagt gccagtgatg cggagcatct
     2521 tccatatccg ggccatgatc ggtgatcgca aaaagtttaa tgcccttttg tttggcctgg
     2581 gcaatgtaat cactcaatgt gctatatgca tgtgtgctgg caacggtatg catatgaagg
     2641 tcgacgggat acataactct ctcctgtatt caattcccgc caaggatagc aggaatcctg
     2701 atgctttatt agtagccgcg tgcgcggtcg acttgcccgc agaccctctc ccctttttcg
     2761 agctgggcaa tggtgcgaga aatgtactcc acagcttcag cgggacgggt aatcgcggcg
     2821 acatgtggtg ttatcgtcac gcgtggatgt tgccagagcg gactttcagg cggtaagggt
     2881 tcacgattaa aaacatccaa cattgcgcct ttaactttgc cgctatccag cgccgcgagc
     2941 aggtcatctt ccacaacatg aacaccacgc gccaggttga ggagatacgc gccatccggt
     3001 aatttttcga gtaattgttg attaataatg ccgacggttt caggggtatt cggtaacaaa
     3061 ttaatcaata cccgacattg gctcagaaat gcagacagtt cttcccgtcc ggcaaagctt
     3121 tgcacgccag gccacgattt acgggttcga ctccagcaac gcagcggaaa gcgccaggtt
     3181 tgcagactct gagcaacttt actgcccagt acgcctgcgc ccaaaatgcc gatggtaaaa
     3241 tcttcccaat gatattcagg cagcggttgc caatgcgaac tattttgctg gatgcgataa
     3301 tcgtcaaaac gtcgaaacca atgcagcacc tgactgacag catattcctg catttgctcg
     3361 cccataccag tatcttccag gcgaaaaagt ggaacagaag ggttcagcat ttcagggtgt
     3421 gcctgtagct tgctcaaaat agaatcaaca ccggccccga gtgcgaacac cgctttaaga
     3481 tcgcgccctg ccagcatttc aacaggagga tgccagacta aagcataatc agcagagtca
     3541 ttatctccgc ttttccatgc tctgactctt gcctgaggaa tagctttgcg cagtgcctca
     3601 atccaccatt gggtatcgaa cgttgggtga taaaagatga tatccatact gactcccgaa
     3661 aagcgttgtg cgaaatttat tcgcacttat cgttatgatc tacaaaggcc accagcataa
     3721 caaatccgtg gtcggtggca aaaaaagcag atttcgctta ttaaaaccac acattgattg
     3781 aagtttgaat aaacgcgcga ttttttcaaa aagtttgttg acctcaggtc atgatttccc
     3841 taaattagcg cccgttccag caagacagga acaacaattt ggtgaggtgt ccgagcggtt
     3901 gaaggagcac gcctggaaag tgtgtatacg gcaacgtatc gggggttcga atccccccct
     3961 caccgccata tttaaagaag agctcgtacg aaagtacggg cttttttttc gtatattgca
     4021 cacaccgggg ggatgagaag ccccgaccgg ggttcgacaa ctggcgacag ccagttggac
     4081 agaccgtgaa cgcagtgagc gggctgcccg cagggcgagc gaagcgagtc aatccccccc
     4141 tcaccgccat ctttaaagaa gagctcgtac gaaggtacgg gctttttttc gtatattgca
     4201 cacaccgggg ggatgagaag ccccgaccgg gatacttagt taatgcaccg gttcactctt
     4261 tgtcgccgcg actaatgggc gaattttgtc acgagtctcc cagaccggag gaatttcctc
     4321 caatttagtg acagaagctc gtacaaaagg cacccgtgca agagtcataa ttgtatccaa
     4381 ccaacggtca cgaataatgc gatccgccct ttcatgactc gggtcatcca gctcaataac
     4441 acagactatt ttattgttct tctttgaaac cagaacataa tccattgcca tgccattata
     4501 aattgcaaag aaacgctttc tttcttcttc gctaaactca ggttgttctg gaaagttgat
     4561 caagcgcccc agagagacct ggcaatggat atgacaataa tgtccaaacc atttttccag
     4621 attatcgaaa tacatttttt cacaacgagt gattaaccac tcttttcttt ccaataaaat
     4681 accttcggca accattccct gaaataactc atctcgctct tgccaacgag agtcacgata
     4741 ggttttctgc ggtatatagc tccttgtcga cgccttagcg gcaggggaag ttgcagatga
     4801 agggacgggt tgcgatttag cgatgattac cgatgaggca acttttcgga gtgtcaattt
     4861 tctggcaaca gcaaaaccgg cgataaatgc aattgtcaaa caagttacaa aagccataaa
     4921 gctcataatt aattttcacc tatccatgtt gattatattt cattaccaat gttactcgct
     4981 gcagcgtgtt acatgccaac aatgcaacga tcaatatata gttagtatca tgttttgatt
     5041 attacaacca tccctattta gccattgcgg cttgccttcc acaaaaaaat aacaacacac
     5101 aatattagca aagaattaat taagattaac cttacattta taatattatc cctaaagaat
     5161 aaatcagagg aatatattcg gtgttaaaca gtcagtcaaa gtatagccca ttgttgatta
     5221 aacctcataa gcaaaattgg caccagatct cctgtacagg ttgaatggcc gaaaaaagat
     5281 aggcagatgc tcattttttt ggcttctgga agaaaaactc tccgcgctgg cagtattaca
     5341 ctggtctgat gctatgacga aaaatgcctg gtaagagtat ctaaaccaga ctccagttca
     5401 gcttatcaac acgctcaaag tcgcccccct gaacgctaac ctgaagtgaa aatctgttga
     5461 aataaaacaa agcccggttc gcccgggctc tgcaccgata acacacttaa ctgtatgcat
     5521 gcagcgtacg ttggcaaagt gccgaacgta cgcaatcctc ttttccgaac cggacgatcc
     5581 caaccatttc atcttcttcg aaacgttcca gcgcgtcact taatccggag cacacgccgc
     5641 gaggcaaatc gcattgcgtg atatcaccgt tgacgataac cgtcacgttc tccccgaggc
     5701 gggttaaaaa cattttcatt tgcgcggcag tcacattctg cgcctcgtca agaatgacga
     5761 ctgcattttc aaaggtacgt ccacgcatat aggcgaacgg cgcgatttcc accttcccta
     5821 tttccggtcg caggcagtac tgcataaagg aagcccctaa gcgccggacc agcacgtcgt
     5881 agaccgggcg aaaataggga gcaaactttt ctgcgatatc accaggtaag aagccaagat
     5941 cttcatcggc ttgcagaact ggacgggtga cgataatcct gtcgacatcc ttatgtatca
     6001 gggcctctgc cgcttttgct gcgctgatcc aggtttttcc gcacccggct tcgcccgtgg
     6061 cgaatatcag ctgcttactc tcaatagcct tcagatagtg caattgcgct tcatttcgcg
     6121 cgaggatggg cgaggtatcg cgactgtcgc gggccatacc aatggcttct acgccgctca
     6181 tctgcacaag cgaggtgacc gattcttctt cacgctgctt atggctgcgc gaatcccgtc
     6241 tcagcacacg ttttgcctcg cgacgagctt tgatcactgc tttttgtctt cccatggaga
     6301 gcaccttgag ttgtttgtat tcatcacacg cgccgttggc agcgcgatta tgcgcacgaa
     6361 catcagaggg ttggcttcct tgtaagccat agtttgcttt tggataaaat gccgaaaaac
     6421 ggctacgcgc accgtttacg gcgtcggtaa cacatgaaaa gaaaggatga ggttgaaaat
     6481 gcaaagtgac gagatgacta ccggaggaga aaactccgcg agtggtggcg cgttgattat
     6541 ctaaaacatg tccagtacag gacgttacca tccgcgatct ccatagtgac tgactatcac
     6601 tgccgggaac ttccgctgct acttaataag tacaacagat ctcgcatttt attgcaacaa
     6661 tatatttact tatatttaac tataaaacac tatttcagtg acattagttt ctactggaaa
     6721 gatgacagag tgatgacagt gatgaaaaaa gctgtgtgct ttcagcagga tttgcatcag
     6781 gcatcagtgc acgatcgccg gatgcggcgt gaacgcctta tccagcctac caaacccgca
     6841 ctgattgtag gcatgataag acgcgcaagc gtcgcatcag gcatctgcgc acaaatgcct
     6901 tatccgccta aaaacattaa acccgcaata acgcgcttcc gagataatcg ttcgcgtcct
     6961 tcactcccgg cagcgaaaaa aaataaccgc cgccgatagg tttaacgtat tcctccagcg
     7021 cttcgccatt gagccttttt tgtactgtca ggaagccttt ttccagatcg tgttggtagc
     7081 agacaaacag caaccccata tccagttgtc cggagttggt gacccccaac gaataactgt
     7141 agccgcgacg cagcatcagg ctggactcac tctccgccgt gcggggattc gccagccgga
     7201 tatggctgtc cagcgcgatc accttccctt ccgggtcgct ggcgtaatca ggcacatcat
     7261 gctcatgctg cattcccagc ggcgcaccgg tttgcttatc acggccaaaa atcgtctgct
     7321 gttctttcag cggcgttctg tcccaaaatt ccactcgaaa ctgaatcaag cgtactgcct
     7381 gatagctgcc accgattgtc cacgcaggct cctgctgatc tgccgttacc cacaccactt
     7441 tttgcatcaa cttatcattc tggctatcgg gattggcagt gccgtcttta aagcccagca
     7501 aattaatcgg cgtctctttg cctttactac gcgctgcgtg atcggaaata aacccttccc
     7561 gcttccagcg cacgctgagc aaatccggcg tgtgtttgat gatatcgcgc agcgcatgga
     7621 tcaccgtgtc ctgggtgttg gcgcaaattt gtagcaacac atcaccatgg cataacgccg
     7681 catccagcga gtcgttgggg aaacgcgtca tcttctgcaa ctttttcggc atctgtggcg
     7741 caaggccaaa ccgctcatca aacaatgagt gacccaccga taacgtgatg gtgagattat
     7801 cgggcgcaat gtagccgcca agaatgccgg aatcgagtgg tggcaggcgc ggatttggcg
     7861 tttctggtgc cgctccgccc tgagtcagaa aagcaaaacg ctgagtcaac aagcgaaaca
     7921 accgttcaag atcggcttta tcgctggcaa gcacatcaaa cgccaccagc atcattgcgg
     7981 cctgttgtgg cgtcaggatc cctgcctgat gctcaccata aaacggctgt ttctcattgc
     8041 gtgcatccgg tgaaagcgta cccggcgcac tttgcgtttt ttgtgcatga gcgaccggac
     8101 aacttcccgc cagcgccagc gcacctatca ctttcagtaa acgtcggcgt gacggttcat
     8161 tcacgccgtt ttcatcttta tactgcataa cgcttaatcc agtcccagca caccgcgaag
     8221 ttgcgccaga tcttccgcct gcgcagtaat cggtcctttc agtgcattcc ggtcggcatc
     8281 ggtcaatttg tcgtaggttt caaaaccgtc tttagtacgg tatttcgcca gaatggtatc
     8341 gaccttttta aagttggcat cgacttttgc cagcagttcc gggttggctt tttgcagttg
     8401 tggacgcagc aaatcgacaa ttttctgcga gccttcaacg ttagcctgga aatcccacag
     8461 atcggtgtgg ctgtagcgat cttcttcacc gctaattttg ctggctgcca cttcctcaat
     8521 cagtccggct gcgccgccga ccacttttga aggtgggaaa gccagttcac tgatgcgttt
     8581 ttgcaaatcg accacatctg tataaagctg ctcagcgtac tgatccatcc ctttggtggt
     8641 gttgtcgcca aacaatgctt tttccagacg gtggaaacca gtgaattttg ggtcggcggc
     8701 tttttgctcg taatcatctt cacgggcgtc aatgctgcca tccagatcgg agaacagttc
     8761 agcaatcggt tcaatgcgct catagtgctg gcgcgtcggc gcatacagtg ctttcgcttt
     8821 ttcgatatcg ccagctttaa tcgcgtcggt aaaggctttg gtgtcggtca ccagctgcgt
     8881 ggtttccgcc atgacatacg ctttatatgc agtaattgca ccaccaagac ttaacagcgc
     8941 atcactttgc gccgcatccg ccgttgcctc acctttgacg atcaacttcc ctttcgggtt
     9001 agtcagcaga ccgcaggtca tatcgtattc gccaggctgt aaattcgccg tcattttctg
     9061 gctaaaacca ggggcgatat tttcccgctc ttccaccacc atcacgcctt tgaggatctc
     9121 ccactccagc gccttctggc tatggttctg aataatgaac tgtgttttcc cggcgttaac
     9181 cgtaatggtc atcggttcgc actgcttatc cgtcacggtc actttgacct gcggcacatc
     9241 agcggcgtta gccataaaag cagaagaaaa cagcgcagcc acgctcaact gcaatgcgtt
     9301 acggcagaag ttaatggtca tgacgagact atccctttaa agtatgttgt aactaagcaa
     9361 gagtttgcgt cgtatttgtt acgcggagcg agacgctgtc gcccctgcgc gtggtggcag
     9421 agcaaatgcc accagcgccg ggatgagata aataaaccag acggcgactt cgctgacgct
     9481 cggcgcttcc tgatagccaa aaatcccttc catcagcgtg ccaaacagcg agtgagttga
     9541 gagcaccgca ctcatatcga aggcgatttc ctgaaagtgg ttccacaatc cggcttcatg
     9601 aaatgcgcga atggcaccag ctgccagccc tgcggcgacg aagagaataa acaggctggt
     9661 ccatttaaaa aatgcaccaa gattgaggcg aataccgccc cagtagagca gaaagccgag
     9721 caccacggct gtagcaagac cgagcattgc acccagcggc ggccagatcc cgacatcttg
     9781 ttgaaatgcc gccagcagga aaaagaccga ctccagccct tcccttgcaa cggcaaaaaa
     9841 gaccatcatc accagcgccc agccatgatg atttccacgc tacaatgcgc tatcgactgc
     9901 ctgttccagt tgcacttacg ttgcgcgaca ctttgcgcat ccagaaaacc atccaggtaa
     9961 ggatcaccac ggcgatcacc gccacgatac cttcaaacag ttcctgttct ttttgcggaa
    10021 attcgccggt ggtttcgtta atgaagatcc ccaggcccag gcacaacgca gcggcaagca
    10081 acacgccaat ccacatcaca ccaatccatc ggcctcgctg ggtacgctta agatagctgg
    10141 caatcaaact gacaatcagc gcggcttcaa gtccttcgcg caacataatg agaaacggaa
    10201 caaacatgcc acccaccctt aaacgttttt ctgagtcaat ctatgcaaag aaaagtaaat
    10261 cgcagtgata gcgattatca ttacggtaaa gaaaaataca agcggaatgt ttataaaatg
    10321 atgttggaat gtaaacaaag agaggtaatg ccaggggata taaacgatta ttccagggta
    10381 gaaagatggg attgtttgca aagaaccatg tgttcttgcc ggatgccgcg taaacgccat
    10441 atccagccga caaaactgtc cagactcaat gcatcagagc ttccatgtaa aactgataag
    10501 cgggtgtatc aagcaatttc gtccatccgc gattatcaaa tctaactttt tgcccggaac
    10561 ataataacag ctcgggtgtt ggtggcgtca aaatcgctga agcgttcccc gaaggccggg
    10621 acagcccaca tggatgtggg ctgagggcgc gtcttacagg acgttacctc gcgcccgacc
    10681 cggtagccga aggggataag ctgaaggtac cgcgcagcgg cgattttgtt cgccagagcc
    10741 cgggggtgca cagggggcgg cggcgattgg ccgccccctg cgcgctccct gcaccattca
    10801 atttatatgg cgaggcacat taagggagcg caacccgctg ctgaagccaa acaaaaccgc
    10861 cgcaggctaa gtcccttagc tttcctgcaa ccgtgacggc ggagccgaat gatagtgcgc
    10921 atcggcctcg gcaaagcgtt tttgcatcgc cgctgacggc gctttaccca gcaaactaaa
    10981 cactacaatc ccgatactgc cgaagatgaa gcccggaata atttcgtaca gtcccagcca
    11041 gccgaactgc ttccagacga taaccgtcag cgcaccgatg atcatccccg ccagtgcacc
    11101 gttacgcgtc atgcgtgacc acatcaccga gaacagcacc accggaccaa acgccgcgcc
    11161 aaagcctgcc cacgcgtagc tcactaagcc cagcacgcgg ttttccgggt ttgccgccag
    11221 cgcaatcgcc accagcgcca ccaccagcac catcacacgc cctacccaca ccagctcttt
    11281 ctggctggca tgtttacgca gaaacgcttt gtataaatct tcggtaatcg cactggagca
    11341 caccagcagt tggcaactta aggttgacat taccgccgcc agaattgccg acagcagaat
    11401 cccggcaatc cacgggttaa acagaatttg cgccagttcg ataaacacgc gctcggcgtt
    11461 ctggtttacc gcgcctgcta ccgccgggtg ctcattaaag taagcaatcc caaagaagcc
    11521 gacagccacc gcccctgcca ggcagaggat catccaggtc atactgatac gacgcgcatg
    11581 gacaatgctg tggtgagaat ccgccgccat aaaacgcgcc agaatgtgcg gctgcccgaa
    11641 gtaacccagc ccccaaccca tcagtgagat aatggcaaca aagttcagac ctttgagcat
    11701 atcaacgttt tcgatgctct tttgtttgat cacttccagc gagtcaccaa agccaccgac
    11761 actgataatg acgataaccg gtgtcaggat cagggcaaaa atcatcaggc tggcctgtac
    11821 agtgtcagtc cagctcaccg cgaggaaacc gccaataaag gtgtaaagga tcgtcgccgc
    11881 agctcccgcc cacagagccg tttcgtagct catgccaaag gtactttcaa acagacgcgc
    11941 gcctgccaca atgcccgaag cgcaataaat ggtgaagaac agcaaaataa ccagcgcgga
    12001 gataatgcgc aaaatgcggc ttttatcttc aaaacgcccg gtgaaataat ccggcaatgt
    12061 taaggcgtta ttattgtatt cggtatgcac acgcaaccgc ccggccacca gcttccagtt
    12121 gatccacgcg cctaatgtca ggccaatggc gatccagctt tcggaaatcc cggaaagaaa
    12181 aaccgcgcca ggcaacccca ttaacagcca gccgctcata tccgacgcac ccgccgataa
    12241 tgccgtcaca aatggcccaa gactacgacc gcccagaata tagtcgtcaa agtttttcgt
    12301 tgatcgccag gcgataaacc caatcaatat catgccaaag atatagacac aaaatgtcac
    12361 caacatcggt gtgctaatag ccatctaaag tctccaaaaa attattatcg gcaatgtcga
    12421 aacttgccgt tatatctgca accggaacgg ggtaacagag tttatttttt accagggcga
    12481 ccgtatcctg ccggaagcgc tggttattca caatcgattt aacacaccat ttacattaaa
    12541 ttttagtgct cagcgacact atttttcatc aggttgcact ctctcacatt ttttgcggtt
    12601 gcacctttca aaaatgttaa ctgccgcaga gaaaaagtct gagttatttt tttaatccct
    12661 gtcatatcaa tttcttttat taacatttca ttcattttta agcttgctac gcaggtcaca
    12721 tttaacatgg ttgcacaaag ttgcaacatc atggatattt cacgataacg ttaagttgca
    12781 cctttcagaa caacaggagt aatggcatgg gaaccaccac catgggggtt aagctggacg
    12841 acgcgacgcg tgagcgtatt aagtctgccg cgacacgtat cgatcgcacg ccacactggt
    12901 taattaagca ggcgattttt tcttatctcg aacaactgga aaacagcgat actctgccag
    12961 agctacctgc gctgctttct ggtgctgcca atgagagcga tgaagcaccg actccggcag
    13021 aggaaccaca ccagccattc ctcgactttg ccgagcaaat tttgccccag tcggtttccc
    13081 gcgccgcgat caccgcggcc tatcgtcgcc cggaaaccga agcggtttct atgctgctgg
    13141 aacaagcccg cctgccgcag ccagttgctg aacaggcgca taaactggca tatcaactgg
    13201 ccgataaact gcgtaatcaa aaaaatgcca gtggtcgcgc aggtatggtc caagggttat
    13261 tgcaggagtt ttcgctgtca tcgcaggaag gcgtggcgct gatgtgtctg gcggaagcgt
    13321 tgttgcgtat tcccgacaaa gccacccgcg acgcgttaat tcgcgacaaa atcagcaact
    13381 gtaactggca gtcacacatt ggtcgtagcc cgtcactgtt tgttaatgcc gctacctggg
    13441 ggctgttgtt tactggcaaa ctggtttcca cccataacga agccagcctc tcccgctcgc
    13501 tgaaccgcat tatcggtaaa agcggtgaac cgctgatccg caaaggtgtg gatatggcga
    13561 tgcgcctgat gggcgagcag ttcgtcacgg gtgaaaccat cgcggaagcg ttagccaatg
    13621 cccgcaagct ggaagagaaa ggtttccgtt actcttacga tatgctgggc gaagctgcac
    13681 tgactgccgc agacgcacag gcgtatatgg tttcctatca gcaggcgatt cacgccattg
    13741 gtaaagcgtc taacggtcgt ggcatctatg aaggtccggg catttcaatc aagctgtcgg
    13801 cgctgcatcc gcgctacagc cgcgcccagt atgaccgggt aatggaagag ctttacccgc
    13861 gtctgaaatc actcaccctg ctggcgcgtc agtatgatat tggtattaac atcgatgccg
    13921 aagaggccga tcgtctggag atctccctcg atctgctgga aaatctctgt ttcgagccgg
    13981 aactggcagg ctggaacggc atcggttttg ttattcaggc ttatcaaaaa cgctgcccgt
    14041 tggtgatcga ttacctggtt gatctcgcca cccgcagccg tcgccgtctg atgattcgcc
    14101 tggtgaaagg cgcgtactgg gatagtgaaa tcaagcgtgc gcagatggac ggccttgaag
    14161 gctatccggt ttatacccgc aaggtgtata ccgacgtttc ttatctcgcc tgtgcgaaaa
    14221 agctgctggc ggtgccgaat ctaatctacc cgcagttcgc gacgcacaac gcccatacgc
    14281 tggcagcgat ttatcaactg gcaggtcaga actactatcc gggtcagtac gagttccagt
    14341 gcctgcatgg tatgggcgag ccactgtacg agcaggtcac agggaaagtt gccgacggta
    14401 aacttaaccg accgtgtcgt atttatgccc cggttggcac atatgaaacg ctgctggcgt
    14461 atctggtgcg tcgcctgctg gaaaacggtg ctaacacctc gtttgttaac cgtattgccg
    14521 acacctcttt gccactggat gaactggtcg ccgatccggt cactgctgta gaaaaactgg
    14581 cgcaacagga agggcaaact ggattaccgc atccgaaaat tcccctgccg cgcgatcttt
    14641 acggtcacgg gcgcgacaac tcggcagggc tggatctcgc taacgaacac cgtctggcct
    14701 cgctctcctc tgccctgctc aatagtgcac tgcaaaaatg gcaggccttg ccaatgctgg
    14761 aacaaccggt agcggcaggt gagatgtcgc ccgttattaa ccctgcggaa ccgaaagata
    14821 ttgtgggctt tgtgcgtgaa gccacgccgc gtgaagtaga acaggcgctg gaaagtgcgg
    14881 ttaataacgc gccaatctgg tttgccacgc ctccggctga acgcgcggcg attttgcatc
    14941 gcgctgccgt gctaatggaa agccagatgc agcaactgat tggtattctg gtgcgtgaag
    15001 ccggaaaaac cttcagtaac gctattgccg aagtgcgcga agcggtcgat tttctccact
    15061 actacgccgg acaggtgcgg gatgatttcg ctaacgaaac ccaccgtcca ttagggcctg
    15121 tggtgtgtat cagtccgtgg aacttcccgc tggctatttt caccgggcag atcgctgccg
    15181 cactggcggc aggtaacagc gtgctggcaa aaccggcaga acaaacgccg ctgattgccg
    15241 cgcaagggat cgccattttg ctggaagcgg gtgtaccccc tggcgtggtg caattgctgc
    15301 caggtcaggg ggaaaccgtg ggcgcgcaac tgacgggtga tgatcgcgtg cgcggggtga
    15361 tgtttaccgg ttcaaccgaa gtcgctacgt tactgcagtg caatatcgcc agccgcctgg
    15421 acgctcaggg tcgccctatt ccgctcatcg ctgaaaccgg cggcatgaac gcgatgattg
    15481 tcgattcttc agcactgacc gaacaagtcg tcgtggatgt gctggcctcg gcgttcgaca
    15541 gtgcgggtca gcgttgttcg gcactgcgcg tgctgtgcct gcaagatgag attgccgacc
    15601 acacgttgaa aatgctgcgc ggtgcaatgg ccgaatgccg gatgggtaat ccgggtcgcc
    15661 tgaccaccga tatcggtccg gtgattgata gcgaagcgaa agccaatatt gagcgccata
    15721 ttcagaccat gcgtagcaaa ggccgtccgg tgttccaggc ggtgcgggaa aacagcgaag
    15781 atgcccgtga atggcaaagc ggcacctttg tcgccccgac gctgatcgaa ctggatgact
    15841 ttgccgaatt gcacaaagag gtctttggtc cggtgctgca tgtggtgcgt tacaaccgta
    15901 accagctacc agagctgatc gagcagatta acgcttccgg ctatggtctg acgcttggtg
    15961 tccatacgcg tattgatgaa accatcgccc aggtcactgg ctcggcccat gttggtaacc
    16021 tgtatgttaa ccgtaatatg gtgggcgcag tggttggtgt gcagccgttc ggcggcgaag
    16081 ggttgtccgg taccgggccg aaagcaggcg gtccgctcta tctctaccgt ctgctggcga
    16141 atcgcccgga aagtgcgctg gcagtgacgc tcgcgcgtca ggatgcagag tatccggtcg
    16201 atgcacagtt gaaagccgca ttgactcagc cgctaaatgc actgcgggaa tgggcagcaa
    16261 atcgtccaga attgcaggcg ttatgtacgc aatatggcga gctggcgcag gcaggaatac
    16321 aacgattgct gccggggccg acgggtgaac gcaacacctg gacgctgctg ccgcgtgagc
    16381 gcgtgttgtg tattgccgat gatgagcagg atgcgctgac tcagctcgcc gccgtgctgg
    16441 cggtgggcag ccaggtactg tggccggatg acgcgctgca tcgtcagcta gtaaaggcat
    16501 tgccatcggc agtcagcgaa cgtattcaac tggcgaaagc ggaaaatata accgctcaac
    16561 cctttgatgc ggtgatcttc cacggtgatt cggatcagct tcgcgcattg tgtgaagccg
    16621 ttgctgcgcg ggatggagca attgtttcgg tgcagggttt tgcccgtggc gaaagcaata
    16681 tccttctgga acggctgtat atcgaacgtt cgctgagtgt gaataccgct gccgctggcg
    16741 gtaacgccag tttaatgacg atcggttaaa caggtagccg gaggatgtta caccctcctc
    16801 cggcatcttt aacgtggtcg aatcccctca ataataatcc gctgcacgtt ttcaaccgtt
    16861 tgattgaaaa atacctcatc gcgcaacgtt gcgcctgtca ccgcctccac ctgaggggcg
    16921 aaatcggcgt aatgttgagt ggaagcccaa ataataaaaa tcaaatgctg cggatcaatc
    16981 ggcgcgagtt tgccgctttt gacccaaccg gcaatcagcg ccgatttctc atcaattaat
    17041 gccttcaaat cgcccgtcag ttcatccatt aacagcggcg cgcctgccag catctccata
    17101 cagaacaggc gcgaagcctg cggataatcg cgtgagactt ccagcttcag acggatgtac
    17161 tctttgatcg ccgccagcgg ggcgaaatct tcacgaaacg cttttaacgg tgccagccag
    17221 atatcgagaa tctgccgcag cacggcaata tacagcgcct cttttgacgg aaagtaatac
    17281 agcagattgg tttttgaaac acccgccaac tctgcgatct gctccagcct tgtgccgtga
    17341 aaaccgaatt gtgaaaaagt gtccagtgct gcgctaagaa tcgctttttt cttcgcgctt
    17401 actgcgcgcg aacgtttacc cgttgttttc actgcgcctt gcgtcatgcg ctctcccctc
    17461 ttgtagattg gcaacaggat agcaaaaccc ccgcccggcc tcgaccttct gcactctcat
    17521 cgcgcacgga tgcatgtttt atgtgcaact gttttgaccg tttagtccac tttttaccag
    17581 atattttaga taacacaatg ctaacacatt aataacattc actttttaaa aactggcatc
    17641 cgctttgcaa acaagccaat acgcggctga aaaggatatg caggatgcag cgccccgtct
    17701 gactttcact ttacgagacg aagagaggtt gatgatgaaa attggcgtat tcgtacctat
    17761 tggcaacaac ggctggctca tttcgaccca cgcgccgcag tacatgccga cctttgaact
    17821 gaataaagcc attgtgcaaa aagcggagca ttaccatttc gatttcgccc t
//
LOCUS       DAZPEB010000066        17800 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZPEB010000066 DAZPEB010000000
VERSION     DAZPEB010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17800
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.067"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..388
                     /locus_tag="ACHURJ_001997"
     CDS             <1..388
                     /locus_tag="ACHURJ_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFR1280952.1"
                     /translation="FLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSFVVPLAFVKNLD
                     KTLTVNTGALLTMSVSANGGMPPYKYAWKKDGQPVDGQTTDTFSKPGAQSADAGKYTC
                     VVTDSAEKAQSVTSVECTVTVSAAAG"
     gene            403..777
                     /locus_tag="ACHURJ_001998"
     CDS             403..777
                     /locus_tag="ACHURJ_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFR1280953.1"
                     /translation="MKKDLKTLALARLSGFRHKTVKVPEWGNVSVVLREPSAEAWYLW
                     QEVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHARLLRQALELIADAESARKK"
     gene            873..1082
                     /locus_tag="ACHURJ_001999"
     CDS             873..1082
                     /locus_tag="ACHURJ_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HFR1280954.1"
                     /translation="MWMEFDRISPLGDERGDIRNAQIVKAVFGAQGMNVALKDVMLCW
                     GEDEDKPEVDPFAALEDALSFAAQS"
     gene            1130..1390
                     /locus_tag="ACHURJ_002000"
     CDS             1130..1390
                     /locus_tag="ACHURJ_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1280955.1"
                     /translation="MATLRELIIKIVSFRGTHIDAFFRRASSAAHTNARGLITVDLFC
                     CTEAIARQVPEAGSEVLSSVIASGREVLTRGLIAGDDVLTRE"
     gene            complement(1525..3126)
                     /locus_tag="ACHURJ_002001"
     CDS             complement(1525..3126)
                     /locus_tag="ACHURJ_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc49 family transposase"
                     /protein_id="HFR1280956.1"
                     /translation="MNIRIWSGILPCMDISALNTTNDIEKLRAMALAMVQEVMSENAE
                     KERELLEKSRRIQLLEEMLKLVRQQRFGKKCETLAGMQRSLFEEDVDADIAALTAHLD
                     KLLPQSPEEDEKASRSRPIRKPLPVHLPRVEKMIQPDTDHCPECDEPLHYIRDAVSEK
                     LEYIPAHFVVNRYVRPQYSCPCCQKVFSGEMPAHILPKSAVEPSVIAQVIINKYGDHL
                     PLYRQQQVFARSDVGLPVSSMADMVGAAGAALSPLAALLHRELINRPVVHADETTLKI
                     LNTKKGGKSCSGYLWAYVSGERTGPSVVCFDCRTGRSHEYPENWLQGWGGTLVVDGHK
                     AYRTLANKVPEITLAGCWAHARRGFADLYKISKDPRAAIAVKKIAGLYRLEKKISSRP
                     VEKIRQWRQRYARPILEELWSWLEEQEPQCSPGKALHKAIAYALSHRVELSRFLEDGA
                     VPLDNNVCERAIKNVVLGRKSWLFAGSQMAGERAAQIMSLLETAKRNGLEPHAWLTDV
                     LMRLPEWPEERLAELLPLEGFTFSG"
     gene            complement(3133..3525)
                     /gene="tnpB"
                     /locus_tag="ACHURJ_002002"
     CDS             complement(3133..3525)
                     /gene="tnpB"
                     /locus_tag="ACHURJ_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950651.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR1280957.1"
                     /translation="MLSPDNVFIAIKPVDMRRGIDSLTQYIQDELRSTWHEGAAFVFV
                     NKVRSRIKVLRWDKHGVWLCTRRLHKGSFRWPRANDAAWHLTPDEFNWLIAGVDWQQV
                     KGHDLTKWVWQNEPELRPENTKNTLLTQ"
     gene            complement(3512..3841)
                     /locus_tag="ACHURJ_002003"
     CDS             complement(3512..3841)
                     /locus_tag="ACHURJ_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR1280958.1"
                     /translation="MSKPRWTIEQKRQHVAAWRASGLTRQQYCELNDIPFTSLREWPQ
                     DVAKAERRANEPTVLPVHIAPPLHADVPQPVTNEPVTLFLPGGVRMCCQPSQLTDVFR
                     ALKYAQS"
     gene            3886..7146
                     /locus_tag="ACHURJ_002004"
                     /pseudo
     CDS             3886..7146
                     /locus_tag="ACHURJ_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304285.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
     gene            7139..7480
                     /locus_tag="ACHURJ_002005"
     CDS             7139..7480
                     /locus_tag="ACHURJ_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1280959.1"
                     /translation="MAEIKTLHLVPREGMQVSEKPSVARVRFGDGYEQRRPTGLNARL
                     KTFQAVFRVTDEATRRWLEEFLSWHGGYRAFLWRPPKHNRTVRVVCREWSVTDNARYS
                     DFSCTIEQVVN"
     gene            7480..8178
                     /locus_tag="ACHURJ_002006"
     CDS             7480..8178
                     /locus_tag="ACHURJ_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFR1280960.1"
                     /translation="MQDIHEESLSESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGRKYEAYPIDGSGFEMNGRGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSQLTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWTYRSDECGYTGGAVADEFDKPTTDIRKDRCSKCMRGCELRRNV
                     GNFGGFLSINKLSQ"
     gene            8189..8932
                     /locus_tag="ACHURJ_002007"
     CDS             8189..8932
                     /locus_tag="ACHURJ_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR1280961.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVISTPEGERYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGELPWLSEADRRLQIKSALPWWLVCRGEIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIDMPDFHREDDWWRNGQNLYLDNMAVT
                     GFYRVPLSSAPPGDILLCCFGASVPNHATIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSVWRHRHWHASAFTGICNDLAAASACM"
     gene            8830..9510
                     /locus_tag="ACHURJ_002008"
     CDS             8830..9510
                     /locus_tag="ACHURJ_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFR1280962.1"
                     /translation="MATTNAFCLASPPLARICLHGDLQRFGRRLSLYVNTAAEAIRAL
                     SMQMPGFRRQMNEGWYQIRIAGDDTAPEAVYARLHEQLGEGTVIHIVPRLAGAGKGGL
                     QIVLGAAAIVGSFFTAGASMALWGSALAAGGFSATTMLFSLGASMILGGVAQMLAPKA
                     KTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRDEGGGGK
                     VVVIGRQG"
     gene            9751..13224
                     /locus_tag="ACHURJ_002009"
     CDS             9751..13224
                     /locus_tag="ACHURJ_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1280963.1"
                     /translation="MGKGGGRAHTPREAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPESFESSGSETVLGVEVTRAKPVTR
                     TITSANIDRLRVTFGVQSLVETTSKGDRNPSSVRLLIQLQRNGNWVTEKDVTINGKTT
                     SQFLASVILDNLPPRPFNIRMVRETADSTTDQLQNRTLWSSYTEIIDVKQCYPNTAIV
                     GMQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCL
                     WDMLTHPRYGMGKCLGAADVDKWALYAIGQYCDQTVPDGFGGTEPRMTFNAYLAQQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDVVWPYTNSDVVVDDNGVGFRYSFSAL
                     KDRHTAVEVNYTDPQNGWQTSTELVEDPEAILRYGRNLLKMDAFGCTCRGQAHRAGLW
                     VIKTGLLETQTVDFTLGSQGLRHTPGDIIEICDNDYAGTMTGGRVLSIDAATRTLTLD
                     REVTLPETGTSAVNLINGSGKPVSVDITAHPAPDRIQVSTLPDGVETYGVWGLSLPSL
                     RRRLFRCVSVRENTDGTFAITAVQHVPEKEAIVDNGARFEPQSGSLNSVIPPAVQHLT
                     VEVSAADGQYLAQAKWDTPRVVKGVRFSLRLTSGSGEDSRLVTTAITADTEHRFSGLP
                     LGEYTLTVRAINSYGQQGEPATTTFRINAPAKPATIELTPGYFQITAVPRLAVYDPTV
                     QFEFWFSEAKIADTSQVETSARYLGTGSQWSVSGPHIKPGKDFWFYVRSVNLVGKSAF
                     VEASGRASNDAEGYLGLFREKIGKLHLAQGLWELIDNSQLADEMAEMKTTITETRNEI
                     TQTVSKTLEDQSATIQQIQRVQKDTNDDLAALYMLKVQKTKNGIPYVAGIGAGIEDTD
                     GQPLSNILLLADRIAMINPEDGNTTPLFVAQGNQLFMNDVFLKRLFAVSITSSGNPPT
                     FSLTPEGRLTARNADISGNVNANSGTLNNVTINENCRVLGKLSANQIEGDLVKTVGKA
                     FPRDSRAPERWPSGTVTVRIYDDQPFDRQIVIPAVAFSGAKHEREHTDIYSSCRLIVR
                     KNGAEIYNRTALDNTLIYSCVIDMPAGHGHMTLEFSVSAWLVNNWYPTASISDLLVVV
                     MKKATAGISIS"
     gene            13292..13891
                     /locus_tag="ACHURJ_002010"
     CDS             13292..13891
                     /locus_tag="ACHURJ_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFR1280964.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLHARTNAPG
                     SDNLNGINVKYRYEFTDALGLITSFSYANAEDEKKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF"
     gene            13956..14294
                     /locus_tag="ACHURJ_002011"
                     /pseudo
     CDS             13956..14294
                     /locus_tag="ACHURJ_002011"
                     /inference="COORDINATES: protein motif:HMM:NF019996.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
     gene            complement(14646..14883)
                     /locus_tag="ACHURJ_002012"
                     /pseudo
     CDS             complement(14646..14883)
                     /locus_tag="ACHURJ_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001456549.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14827..15363
                     /locus_tag="ACHURJ_002013"
     CDS             14827..15363
                     /locus_tag="ACHURJ_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HFR1280965.1"
                     /translation="MAGPAGPAGAPGPKGDKGDPGVAGPAGPAGPQGPKGDTGAPGQG
                     TELLTTANTWTQAQTFNGGINGNLTVTGNGSFNDIQIRSDKRNKRNLVKLDNALDRLE
                     ALTGYLYEIQYSADGWQTSVGLIAQDAQKALPELVTEDADVISGEKRLRLNYNGIIAL
                     LVEGFKTLRHEIKELREK"
     gene            15449..15961
                     /locus_tag="ACHURJ_002014"
     CDS             15449..15961
                     /locus_tag="ACHURJ_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1280966.1"
                     /translation="MGVTSGWVGSSAKSETGEQWMGAAGTKLGLSKPFMMSQMVGRTM
                     GCKIATEYYKWKSSDKVDNWGAVGADWPLEEKSKGTITNAASCGSGRLVGAVVTLSHF
                     LTNSTPTAAVYLAGGKAGNITVNVGGATQTMIYQGVVSGFQYYWSGSVSSAFVEAMKK
                     TGVPQDLKIS"
     gene            16034..16249
                     /locus_tag="ACHURJ_002015"
                     /pseudo
     CDS             16034..16249
                     /locus_tag="ACHURJ_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227781.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
     gene            complement(16391..16715)
                     /locus_tag="ACHURJ_002016"
                     /pseudo
     CDS             complement(16391..16715)
                     /locus_tag="ACHURJ_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            16759..16854
                     /locus_tag="ACHURJ_002017"
     CDS             16759..16854
                     /locus_tag="ACHURJ_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HFR1280967.1"
                     /translation="MVCKNVPRLAGELPIVRVLNDFQPLPILRAN"
     gene            complement(16851..17099)
                     /locus_tag="ACHURJ_002018"
     CDS             complement(16851..17099)
                     /locus_tag="ACHURJ_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFR1280968.1"
                     /translation="MRIEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(17362..17457)
                     /locus_tag="ACHURJ_002019"
                     /pseudo
     CDS             complement(17362..17457)
                     /locus_tag="ACHURJ_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(17454..>17800)
                     /locus_tag="ACHURJ_002020"
     CDS             complement(17454..>17800)
                     /locus_tag="ACHURJ_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFR1280969.1"
                     /translation="PAKVLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRR
                     RGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIA
                     RVRSVEEILHAL"
BASE COUNT         4197 a         4291 c         5437 g         3875 t
ORIGIN      
        1 attcctggcg gaagtgcgtc agcacacctg gtcatccggt accaacggtg tggtggctgc
       61 aacgttctca ctgcgtctga aaggcaaacc ggtgtccttt gtggtaccgc tggcgtttgt
      121 gaaaaatctg gataagacac ttaccgtgaa taccggtgcg ctgctgacaa tgtcagtcag
      181 tgccaacggg ggaatgccgc cgtataaata cgcctggaag aaggatggtc agccggttga
      241 cgggcagacg acagacacct tcagtaagcc aggtgcgcag tccgctgatg cggggaaata
      301 tacctgcgtg gtgaccgatt cggcagagaa agcacagagt gtgacgtctg ttgaatgcac
      361 cgtgacagtg agcgcagctg ccggataagg ggatgggtca tcatgaaaaa ggatctgaaa
      421 acgctggcgc tggccagact gtcagggttt cgtcataaaa cggtgaaggt gccggaatgg
      481 gggaatgtca gcgtggtgct gcgggagcct tcggcagagg cctggtatct gtggcaggaa
      541 gtgctcaatg gtgatggaga ggatgacgat accctgtcgg tggtggcgaa aacccgccgt
      601 aacttggaag cggatgtgac gctgttctgc gatgtcctgt gtgatacgga cctgcagcgg
      661 gtgttcactc cggacgaccg tgagcaggtg ctggccgtct atggtccggt acatgcccgg
      721 ttgctgcgtc aggcactgga actgatcgct gatgcagagt cggccagaaa aaagtagccc
      781 gcccggaaat tcgctttctg atgcgacttg cgctccgtct ggggcgcacc ttatccgaac
      841 tgcgccacag cctgagtgcg agcgaggcga tgatgtggat ggagttcgac aggatatccc
      901 cgctgggtga tgagcgcggg gatatccgta atgcacagat cgtgaaagcg gttttcgggg
      961 cacaggggat gaatgttgca ctgaaggacg tcatgctctg ctggggcgag gatgaggata
     1021 agccggaggt ggatccgttt gcggcgctgg aagacgcgct gagctttgca gcacagtcat
     1081 gaatgatgag aaccgctgag gcggtttttt tacgcccgga gaaaggtgaa tggcgacgtt
     1141 acgtgaactg attatcaaaa ttgtaagctt ccggggaact catattgacg cgtttttcag
     1201 gcgcgcgtca tcggcggcgc atacgaacgc tcgtggtttg ataacggttg atttattttg
     1261 ttgcacagag gcgatcgcca ggcaggtacc ggaggcgggc tctgaagtac tgagtagcgt
     1321 gatcgcgtca ggccgcgaag tactgactcg gggattaatt gcaggtgatg atgtactgac
     1381 ccgggaatga ctccgggctg attcaggttc ttattcgctg tcagcccgcg tggttgctgg
     1441 tctggctcat ttcctgccgt caggagttca actcccgact gcaggttatg gcccggtgca
     1501 cgaacacctg acggcaggta tcactcaccc ggagaaggta aatccctcaa gaggcagcaa
     1561 ctctgccagt cgctcctccg gccactccgg cagacgcatc aggacgtctg tcaaccaggc
     1621 atgcggctcc agaccgttgc gtttcgcggt ttccagcaag ctcattattt gcgcggcgcg
     1681 ttctcccgcc atctgcgaac cggcgaacag ccacgatttt ctgcccagaa ccacgttttt
     1741 gatggcccgt tcacacacat tattatccag cggcaccgca ccatcttcca ggaagcggct
     1801 cagttccacg cgatgagaca gcgcataggc aatagctttg tgtaatgcct ttcccggaga
     1861 acattgcggt tcctgctctt caagccatga ccacagttct tccagtatcg gacgggcata
     1921 acgctgtcgc cactggcgga ttttttccac ggggcggcta ctgatcttct tctcaagacg
     1981 gtacaacccc gcgattttct tcacggctat ggcagcccgt ggatctttac tgattttata
     2041 caggtcggcg aagcccctgc gggcatgggc ccagcatccg gccagcgtga tctccggcac
     2101 tttgtttgcc agagtccggt aagctttatg tccgtcgaca accagcgtcc cgccccagcc
     2161 ctgaagccag ttttcaggat actcatggct acgtccggtc cggcagtcga agcacacaac
     2221 tgacggtccc gtcctttctc cactgacgta tgcccacaga taaccggagc aggatttacc
     2281 gcctttcttc gtgttcagga tcttcagggt agtctcatct gcatgcacca ccggacggtt
     2341 tatcaactcg cgatggagta acgccgccag gggagataat gcggcacccg ccgcgccaac
     2401 catgtcagcc atcgaactga cgggcagccc gacatctgaa cgggcaaaga cctgttgctg
     2461 gcgatacaga ggcaggtggt caccgtattt attgatgatc acctgtgcga tgactgatgg
     2521 ctcaacggca cttttcggga ggatatgtgc cggcatttca ccgctgaaca ccttctggca
     2581 acagggacaa ctgtattgcg gacggacata acggttcacc acaaagtgag cgggaatata
     2641 ctccagcttt tcactcaccg catcgcggat atagtgcagc ggctcgtcac attcagggca
     2701 atggtcagtg tccggctgga tcattttttc cacccgtgga agatgaaccg gtaagggttt
     2761 gcgtatcggg cgtgaacggg acgctttttc atcttcttca ggggattgcg ggagcagttt
     2821 atccagatgt gcggtaagcg cggcgatatc ggcatcaaca tcctcttcga atagggagcg
     2881 ttgcatacca gccagcgttt cacatttttt tccgaagcgc tgttgacgaa ccagtttcag
     2941 catttcttcc agaagctgga tgcgccggct tttctccagt aattcccgct ctttttcggc
     3001 attctccgac atgacttctt gtaccatggc aagtgccata gcacgcagtt tttcgatgtc
     3061 attcgtggtg ttgagagcgg agatatccat acaggggagt ataccactcc agatacggat
     3121 attcattatt tttcactgag ttagcagtgt atttttcgtg ttttcagggc gcagttcagg
     3181 ttcattctgc cagacccatt tcgtcaggtc atgtccctta acctgctgcc agtcaacacc
     3241 ggcaatcagc cagttaaact cgtcgggagt gaggtgccag gcagcgtcat ttgcacgtgg
     3301 ccagcggaag ctgcctttgt gcagacggcg ggtacacaac cacaccccgt gtttatccca
     3361 ccggagaact ttgatacgcg aacggacttt gttaacaaag acgaaggcgg ctccctcatg
     3421 ccatgtcgac cggagttcat cctgtatata ctgcgtcagt gagtcgatgc cccggcgcat
     3481 gtcaacaggt ttaattgcga tgaagacatt atcaggactg agcatatttg agtgccctga
     3541 agacatcggt aagttgtgac ggctgacagc acatccgtac tccaccggga aggaagagcg
     3601 tgactggctc attcgtgacg ggctgtggta catcagcatg cagtggtggc gcaatatgaa
     3661 cgggaagaac ggtcggttca tttgcccggc gttcagcttt tgcgacatcc tgcggccatt
     3721 cgcgaagtga tgtgaaggga atatcgttga gttcacagta ttgctgacgt gtaagaccac
     3781 tggcgcgcca ggcagcaacg tgctgccttt tctgttcaat ggtccatcgg ggttttgaca
     3841 tcgattatct ccggtaagtt ggtgaatgga tgtcggtaat actaactgac cgaagatccc
     3901 tgtgaagatg acttccccgt aacgacactc aaaatttcgg caaattcaca gtcattccag
     3961 tcggagatcc agcgggcgtc ccgtatgggc agtgaatatt accggaccct gcagaatggc
     4021 ggacgtcatg ccgctgctgc cgcacgggaa cagcgccgcg cccttgcaga actgaacagt
     4081 cagttgacgg aaattcgcgg ttctgctgtc ggaatggctg gcgcatttgc cggtgccttt
     4141 gccaccggac acctgatttc gctggccgat gagtggggtt ccgtgaatgc ccgtctgaaa
     4201 caggcatctc agtcatcgga tgaattctcg tcatcacaga aagtgctgat ggatatcagt
     4261 cagcgaacag gtaccgcgtt ttcggataat gcggccctgt ttgcccgttc ggctgcctcg
     4321 atgcgtgaat atggttacag tgctgatgat gtgctgaagg tgacggaggc catttcgaca
     4381 ggactgaaac tgtcaggggc tggagttgcg gaatccggtt cggtgatcac ccagttcagc
     4441 caggcactgg cacagggtgt actgcgtggc gaagaattta atgctgttaa cgaaaatggc
     4501 gaccgggtga tccgcgcgct tgctgcgggg atgggtgtgg cccgtaaaga tctgaaggca
     4561 atggcggatg acggaaaact gacagcggat aaagtggtcc ctgcgttaat cagccagctg
     4621 gggatattac gtgatgaata tgcggccatg ccggaaacgg tttccggtag tatcacgaag
     4681 gtggaaaacg cctttatggc ctgggtgggc gaagtgaatg aggcctgcgg ggtgacaaaa
     4741 acgctctccg gcgtgctgaa cggtgttgcc ggacagattg ataatgtggc aacagctgtg
     4801 ggggcgctgg ttgccgtcgg ggttgcccgg tactttggca atatggcctc cggagcgatg
     4861 tctgccacgg caggacttgt gacggctgca cgtaatgaag ttgcactggc ggaagcacag
     4921 ttcaggggaa cgcagattgc cacggcgcgg gcaagggcag ccgtgtaccg tgctcagcag
     4981 gccgtggcgg cagcccgcgg gacggagatg cagattgctg cagaggcccg tctggcggcc
     5041 acacaggaac gcctgaacag aaatattgct gccagaagcg ccgcccagaa tgcgctgaac
     5101 agtacaacgg cggtgggctc acgtctgatg agcggtgcgc tggggctggt tggtggcgta
     5161 cccggactgg tgatgctggg ggctgcagca tggtacacgc tgtaccagaa tcaggagcag
     5221 gcctgggagt ctgcgcgcca gtatgcactg acgatagatg aaatcgcgca taaaacgccg
     5281 tcaatgtctt tgcctgaagc ctcagataat gaaggacgaa cacgggcggc gctgacagag
     5341 cagaaccggc tgattgatga ataggctagt cgggtgaaat ccctgcagga aaaaatcgca
     5401 ggatatcagt atgttctggc gaacccgggc tggacgaccg gtgacggatt catgataaac
     5461 catctgacct cggtgaagac cgtaacggaa gggcttgctc aggcaacaga gcagcttgcc
     5521 gttgagcagt cccgtctggc actgatgcag gaaaaagcgc agtccattca ggatgtgctt
     5581 gccgggctgg aagaccgtcg tgtggcgtta attcgtcagc aggcggcaga gcagaataag
     5641 gtgtaccagt ccatgctggt tatgaacggt cagcatacgg aattcaaccg tctgctgggg
     5701 ctgggtaatg aactgcttca gcagcggcag ggactggtga atgtgccgtt acggctgcca
     5761 caggccactc tggatgataa acagcagagt gccctgacaa aaacagagcg tgagctggcc
     5821 ctgtccagac tgaaagggga agaaaaagag cgtgtccgac tggggtatgc ggcggatgac
     5881 ctcggttttg tgggtgatcc gtatcaggag gcgagacaac gttatatcag taatgccctg
     5941 gaagcctggc gcaataacga ggcgaataaa cccaaatccc ggggtggaaa atcagagacg
     6001 gaaaaagcgg aagacagttt ttcccggctg ctgaagcagc agaaagagca actggcactg
     6061 gcggggcaga atacagagct ggcgaagctg aaataccaga ctgcgcaggg cgaactgaaa
     6121 accctgacgg agatgcagaa gcaggaactg ctgcgcaatg cggccctgat tgaccagcaa
     6181 aaaatccggg agcagttgcg gtcccgggaa gagaccctga agaatgataa tgtggctgct
     6241 cgtgcatcaa atgaagcgga actgctgggg tacgggcagg gagagcggct ccgtgaacgc
     6301 atgcgggagt tgcagcagat ccgcgacagt ttccgccaga aggatgcgga ccttcagtct
     6361 cagtatcaga ccggggatat cagtgaggat ttttacagac aggctctggc acagaacgcg
     6421 cagtatctga gtgaacgtct gaaagagcag gaagcctttt atgccgaatc ggatgtgcag
     6481 cgtgcggact ggcagaaagg gctgcaggag ggattcagta actgggtgga taatgcgtcc
     6541 gattacgcct cacaggcagc acagcttgcg acggagggta tctcagggat ggtgaataac
     6601 atcacggaga tgctgaacgg aaataaagtg gaatggcgca gctgggcctc atcagtgctg
     6661 caggaaatat caaaagttct tatgaatgcc gcgattgtca acgggatcaa gacggcggca
     6721 aacagtatgt ccggagcggg aggatttatc ggcagtattg gtagctggct gggcggtgcg
     6781 gtggccaatg caaaaggcgg tgtgtatacc tcggcaaacc tgagtgcgta cagcaacagt
     6841 attgtggata cgcccacgta ctttgccttt gcaaaagggg cgggactgat gggggaggcc
     6901 ggtcctgaag ccattatgcc cctgacccgg gcggcggatg gctcgctggg tgtgcgagcg
     6961 gtgggcagta tgaacggcag tgcaggtctg gtgtattccc cggtctacca tatcgccatt
     7021 cagaatgacg ggactaatgg ccagataggg ccggaagctg cgggcagcct tgtgcaactg
     7081 attgaccagc gggtgcaggc ggtgatgctg tccatgcgac gtgacggagg aatgctgagt
     7141 ggctgagata aaaacgctgc atctggtccc gcgtgaaggg atgcaggtga gtgagaaacc
     7201 gtcggtggcg agggtacggt ttggtgacgg ttatgaacag cgccgcccga cgggacttaa
     7261 tgcccgactg aagacgtttc aggcggtgtt ccgggtgacg gatgaggcga cccggcgatg
     7321 gctggaagag tttttatcgt ggcatggtgg ttaccgtgcc tttttgtggc gaccgccgaa
     7381 acataaccgg acggtgaggg tggtatgccg ggagtggagc gtcacagata acgccaggta
     7441 cagtgatttc agttgtacga ttgagcaggt ggtgaactga tgcaggatat tcacgaagaa
     7501 agtctgagcg agtcggttaa atcagagcag tcaccgcggg tggtactctg ggaaattgac
     7561 ctgacggtgc agggtggtga gcggtatttt ttctgtaatg agctgaatga aaaaggggag
     7621 ccggtcacct ggcaggggcg taagtatgag gcatacccga ttgacggcag cggctttgag
     7681 atgaacggcc ggggcagcag tgccagaccg tcgctgacgg tgtccaatct gttcggtctg
     7741 gtcaccggga tggcggaaga cctgcagagt ctggtggggg ccacggtggt ccgccgccgg
     7801 gtgtatgccc gttttctgga tgcggtgaat ttcgttgcgg gcaatccgga ggcggacccg
     7861 gagcaggagc tgagtgaccg ctgggtggtg gagcagatgt cgcagctgac agccatgacg
     7921 gcctcgtttg tgctggctac accgaccgag acggacgggg cgctgtttcc cggtcgtatc
     7981 atgctggcga acacctgtat gtggacctac cgctctgatg agtgtggtta cacgggcggg
     8041 gctgtggcgg atgagttcga taaacccacc acggatatcc gtaaggacag atgcagcaag
     8101 tgcatgcgcg ggtgtgaact gcgcaggaat gtcggcaatt ttggcggttt cctttccatt
     8161 aataaacttt cgcagtaaat cccgatttat gacacagact gaatcagcga ttctggcgca
     8221 tgcccggcgg tgtgcgcctg cggagtcgtg cggcttcgtg ataagcacgc cggaggggga
     8281 gcggtatatc ccttgtgtga atatttctgc agagccggag gcgtattttc gtatcgcacc
     8341 ggaagactgg ctgcgggcag agatgcaggg ggagattgtg gcactggtcc acagtcatcc
     8401 cggtgagctg ccctggctga gcgaggctga ccggcggctg cagataaaaa gcgcactgcc
     8461 ctggtggctg gtctgccggg gggaaattca taaattccgc tgtgtgccac atctgacagg
     8521 acggcgcttt gagcacgggg tgacggactg ttacacgctg ttccgggatg cataccatct
     8581 ggcgggaatt gatatgccgg attttcatcg cgaggatgac tggtggcgca acggccagaa
     8641 cctgtacctg gacaatatgg cggtcaccgg cttttaccgg gtgcccctgt cctctgcacc
     8701 gccgggcgat attctgctgt gctgctttgg tgcttcggta ccgaaccatg ccaccattta
     8761 ctgcggcaac ggtgagctgc ttcaccatct gcctgaacaa ctgagtaaac gggagaggta
     8821 ttccgaaaaa tggcaacgac gaacgcattc tgtctggcgt caccgccact ggcacgcatc
     8881 tgccttcacg gggatttgca acgatttggc cgccgcctca gcctgtatgt gaacacggca
     8941 gcggaagcca ttcgcgccct gtcgatgcag atgccgggct ttcgccgtca gatgaacgaa
     9001 ggctggtacc agatacgtat tgccggtgat gacacggcac cggaggcggt gtatgcccgt
     9061 cttcacgaac agctgggtga gggaacggtc atccacattg tgccgcgact ggccggggcc
     9121 ggaaagggtg gactgcagat tgtgttgggg gcggcagcca tcgtgggctc tttcttcact
     9181 gccggggcat caatggcgtt atggggttca gccctggcag ccggtggttt ttctgccacc
     9241 acgatgctgt tttcacttgg agccagcatg attctgggcg gtgtggccca gatgctggcc
     9301 ccgaaggcaa aaacaccgga ttaccgcgca acggataacg gcagacagaa cacgtacttt
     9361 tcctcgctgg ataacatgat tgcccagggg aacccgatgc cggtgcctta cggggaaatg
     9421 ctggttggct cccgccgtat atcccaggac atcagcaccc gtgatgaagg cgggggcgga
     9481 aaggtcgtgg ttatcgggcg acagggataa aacataaaaa aatcccgcag tgatcgcgga
     9541 gctgcgggga cagacaaatg aagatcaatg tgaaggagtt gtttttgtta ctcgggcaaa
     9601 aaaacactaa cgcagcgaaa ttataagcgc cacagtcagt gtgtgaaaat gtgaagatat
     9661 tcagaaattt tattccgtca tgacgcaggc acccggtgag gtgcctgttg tttttgtgag
     9721 tgaacaatta tcacggtaag aggtgatgta atgggcaaag gtggcggcag ggcgcacaca
     9781 ccgcgtgagg cgaaagacaa tctcaaatcc acgcagatga tgagcgtgat tgatgcgatt
     9841 ggtgagggac cgatagaagg cccggtgaaa ggcctgcaga gtattctggt gaacaaaacc
     9901 ccgctgacgg acacggacgg taatcccgtg atacacggtg tgaccgcggt ctggcgtgcc
     9961 ggggagcagg agcagacacc accggaaagc tttgagtcct ccggctctga aactgtactg
    10021 ggtgtcgaag tgaccagggc aaaaccggta acacgcacca ttacgtcagc gaacattgac
    10081 cgcctgcggg tgaccttcgg ggtgcagtca ctggtggaga ccacgtcaaa gggtgaccgt
    10141 aatccgtcct ctgtccgtct gctgattcag ttacagcgta acggtaactg ggtgacagaa
    10201 aaggatgtca ccattaacgg caagaccacc tcacagttcc ttgcgtcggt gattctggat
    10261 aatctgcctc cccgcccctt taacatccgg atggtcaggg agacggcgga cagcaccacg
    10321 gaccagctgc agaacagaac gctgtggtcg tcatacaccg aaatcatcga tgtgaaacag
    10381 tgctacccga acacggccat tgtggggatg caggtggatg cggagcagtt tggtggtcag
    10441 cagatgacgg tgaactacca tatccgcggt cgcatcatcc aggtgccgtc aaactatgac
    10501 ccggaaaaac gcacgtacag tggtatctgg gacggcagtc tgaaaccggc atacagcaac
    10561 aacccggcct ggtgcctgtg ggacatgctg actcacccgc gctacggcat gggaaaatgt
    10621 ctgggggcgg cggatgtgga caagtgggcg ctgtatgcca tcgggcagta ctgcgaccag
    10681 acggtcccgg atggtttcgg ggggaccgag ccgcggatga cctttaatgc gtacctggca
    10741 caacagcgta aggcgtggga cgttctcagt gatttctgct ctgcgatgcg ctgtatgccg
    10801 gtatggaacg gtcagacgct gacgttcgtt caggaccgcc cgtcggatgt ggtgtggccg
    10861 tacaccaaca gcgatgtggt ggtggatgat aacggcgtgg gattccgcta cagcttcagt
    10921 gccctgaagg accggcacac ggcggtggag gtgaattaca ccgacccgca gaacggctgg
    10981 cagacctcca cggaactggt ggaagacccg gaagccatac tgcgctacgg acgcaacctg
    11041 ctgaagatgg atgcgtttgg ctgtacctgc cgcggtcagg ctcaccgtgc aggtctgtgg
    11101 gtgataaaga ccggactgct ggaaacgcag acggtggact tcacgctcgg gtcacagggg
    11161 ctgcgtcaca caccgggtga cattattgaa atctgtgata acgactatgc tgggactatg
    11221 accggcggac gtgtcctgtc cattgatgct gccacccgca ccctgacgct ggaccgtgag
    11281 gttaccctgc cggagacagg tacatcggcg gtgaacctga ttaacggcag tggtaagccg
    11341 gtgagtgtgg acatcaccgc acaccccgcg ccggaccgga tacaggtcag taccctgcct
    11401 gatggtgtgg agacatacgg ggtgtgggga ctctccctgc cgtcactgcg ccgtcgcctg
    11461 ttccgctgtg tctccgtccg ggaaaacacg gatggcacct ttgccatcac ggcggtgcag
    11521 cacgtaccgg aaaaagaagc catcgtggat aacggtgccc gctttgagcc gcagtcaggc
    11581 tccctgaaca gcgtcatccc accggcagtg cagcacctga cggtggaggt gagcgcagct
    11641 gacggccagt atctggcaca ggcgaaatgg gacacgccgc gggtggtgaa gggggtgcgc
    11701 ttcagtctgc gactgaccag cggaagcgga gaagacagcc gtctggtgac caccgctatc
    11761 actgcggata cagagcatcg tttcagtggt ctgccgctcg gggaatacac cctgacagtc
    11821 agggcaatta acagttatgg ccagcagggc gaaccggcca ccaccacctt ccggattaac
    11881 gcgccagcaa aacccgccac cattgaactg acgccggggt attttcagat aacagcggta
    11941 ccgcgtcttg cggtgtatga cccgacggta cagtttgagt tctggttttc ggaggcaaaa
    12001 atcgcagaca catctcaggt ggaaacctct gcccgttatc tggggaccgg cagtcagtgg
    12061 agtgtatccg gcccgcacat taagcccggg aaggatttct ggttttacgt gcgcagcgtc
    12121 aacctggtgg ggaaatctgc ttttgtggag gccagtggac gggccagcaa tgatgcagaa
    12181 gggtatctgg ggctgtttcg ggaaaaaata ggaaaactgc atctggctca ggggctgtgg
    12241 gagctgatag acaacagcca gcttgcggat gagatggcgg agatgaagac caccatcacc
    12301 gaaacccgca atgaaatcac acagacggtc agtaaaacgc tggaggacca gagcgccacc
    12361 atacagcaga tacagcgcgt gcagaaggac acaaatgatg accttgctgc actttacatg
    12421 ctgaaggtac agaaaacaaa aaatggcata ccctatgttg ccggtattgg agcggggatt
    12481 gaggatactg atggccagcc cctgagcaac atactgctgc tggctgaccg tattgcgatg
    12541 attaacccgg aggacggcaa caccacgccg ttatttgtgg cgcaggggaa tcagttgttc
    12601 atgaacgatg tgttcctgaa gcggctgttt gcggtgagta tcacctcgtc cggcaatccc
    12661 ccgacgtttt ccctgacgcc ggagggcagg ctgaccgcaa gaaatgctga tatcagcggt
    12721 aacgtgaatg cgaactccgg gacgctcaac aacgtcacga ttaacgagaa ctgccgggtt
    12781 ctgggaaaac tgtccgcgaa ccagattgaa ggcgatctcg ttaaaacagt gggtaaggct
    12841 tttccgcggg actcccgggc accggagcgg tggccatcag ggaccgttac cgtcaggatt
    12901 tatgacgatc agccttttga ccggcaaatt gttattccgg cggtggcatt cagcggcgct
    12961 aagcatgaga gagagcatac tgatatttac tcctcatgcc gtctgatagt gcggaaaaac
    13021 ggtgctgaaa tttataaccg taccgcgctg gataatacgc tgatttacag ttgcgttatt
    13081 gatatgcctg ccggtcacgg tcacatgacg ctggagtttt cggtatcagc atggctggtg
    13141 aataactggt atcccacagc aagtatcagc gatttgctgg ttgtggtgat gaagaaagcc
    13201 accgcaggca tcagtatcag ctgaatttta taacccatat acgggcgcca gaaatggcgc
    13261 cttttttatt gcagaaaagc gagaggtaat tatgcgtaaa gtttgtgcag ccattttgtc
    13321 cgcagccatc tgtctggctg tatccggtgc gcctgcatgg gcgtctgaac atcagtccac
    13381 actgagcgcg gggtatcttc atgcccgtac gaacgctccc ggcagcgata atctgaacgg
    13441 gattaacgtg aaataccgtt atgagtttac ggacgcgctg gggctgatta cgtccttcag
    13501 ttatgccaat gctgaagatg agaaaaaaac gcactacagc gatacccgct ggcatgagga
    13561 ttccgttcgt aaccgctggt tcagcgtaat ggcggggccg tctgtgcgcg tgaatgaatg
    13621 gttcagcgcg tatgcgatgg cgggtgtggc ttacagccgt gtgtcgactt tctccgggga
    13681 ttatctccgc gtaactgaca acaaggggaa aacgcacgat gtgctgaccg ggagtgatga
    13741 caaccgccac agcaacacgt ctctggcgtg gggggctggc gtgcagttta acccgaccga
    13801 atccgtggcc attgatattg cttatgaagg ttccggcagt ggcgactggc gcactgacgg
    13861 tttcatcgtg ggtgtcggct ataaattctg attagccagg taacacagtg tcatgacagc
    13921 ccgccggttc aggcgggctt ttttgtgggg tgaatatgac agtaaagatt tctggtgtac
    13981 tgaaagacgg cacaggaaaa ccggtacaga actgcacaat ccagctgaaa gcaaaacgta
    14041 acagtaccac ggtggtggtg aacacgctgg cctcagaaaa tccggatgaa gccgggcgtt
    14101 acagtatgga cgttgagtat ggtcagtaca gcgttattct gttggtggag ggattcccgc
    14161 cgtcacatgc cgggactatc accgtgtatg aagattctca acccggtacg ctgaatgatt
    14221 ttctcggtgc catgactgag gatgatgccc gtccggaggc actgcgtcgc tttgaactga
    14281 tggtggaaga ggtggtgcgt aacgcagagg aggcgaagaa gaatgccgga gaggcggaga
    14341 cgtcagcgag gaatgccggc atatcagcca gtcaggcaga agagagcgct gcaaatgctg
    14401 acacttcagc aggggatgca tcggagtcag cccggcaggc ggcagaaagt gcagcctcag
    14461 caaagcagtc agaggaggcg tcctcgtcct cggcctctgc ggccgctcaa aaagccagtg
    14521 agtcattaca aagtgcaaca gatgctgagt tgtcaaaaaa gacggcagaa agtgcagccg
    14581 gtaatgcagt cagggatgca acgaccgcaa cagaaaaagc ccgggagtca gcagaaagcg
    14641 cacagtcagc ggaacaaagc aggatagcgg cggaagaggc cgtaaaccga atccccaccg
    14701 tggtgggacc tcccgggcca aagggggaac cggggcccgc gggtcctcag gggccgaagg
    14761 gagataaagg agagcgtggc gacaccggcc cggcaggggc caaaaggtga caaaggcgat
    14821 ccgggagtgg ctggaccagc aggtccggca ggtgcgccgg ggccgaaagg cgataaaggt
    14881 gatccgggag tagcaggtcc agcaggtccg gcggggccac agggaccgaa gggagacaca
    14941 ggagcccccg ggcaaggaac agaactgctt actactgcca atacatggac tcaggcacaa
    15001 acttttaatg gtggtattaa tggcaatttg acggtgaccg gaaacggctc atttaacgat
    15061 attcagatcc gttcggataa acgcaacaag cgaaatctgg taaaactgga taatgcgtta
    15121 gatcgtctgg aggcacttac tggttatctt tacgagatac agtactctgc cgacggttgg
    15181 caaacgtcgg ttggtttaat tgctcaggat gcacaaaaag cattgcctga actggtaact
    15241 gaagacgcag acgttatatc tggtgaaaaa cgtctgcgtc ttaactacaa cggcataatt
    15301 gcattgttag tcgagggctt taaaacactt cgtcatgaga ttaaagaact ccgggagaag
    15361 taaacgacag ctgttgtagt ttctggtttc tactgaattt aaattgtggg gtgacactca
    15421 ccccacgcat tcagaagggg gagatgagat gggggtaaca tcgggatggg taggctcttc
    15481 ggctaagagc gaaacaggtg agcagtggat gggagccgcc ggaaccaaac tgggactgag
    15541 taaacctttt atgatgagcc aaatggttgg gcgaactatg ggctgtaaaa ttgcaactga
    15601 gtactataaa tggaaatcct ctgacaaggt tgataactgg ggcgcagttg gcgctgactg
    15661 gcctttagaa gaaaaaagta aaggtacaat tacaaacgcc gcaagctgcg gatcagggag
    15721 gctggtaggg gctgtcgtta cactttctca ctttttgacg aactctacac cgacagctgc
    15781 tgtttattta gccggtggta aagcaggtaa catcaccgta aacgtaggtg gtgctacaca
    15841 aaccatgatt tatcagggcg ttgttagtgg gttccagtat tactggtcag gttctgttag
    15901 ttccgctttc gtggaggcaa tgaaaaagac gggagtaccc caggatctaa aaattagtta
    15961 aatggtaaat aaataatttt aaaaacttca cgttctacat accggagacg ccggatatat
    16021 aggatattgt tttaagttgc cagagaaatt tttccggacg gatgctgata ataatgatgt
    16081 gccaatgacg gcagcatcat ggatggcact gagtgaagcg acagagcagg cgatgtttgc
    16141 gaagggagtg gagattaata cacggcagtt gcagatgaaa gccgaggttg aggcgctgac
    16201 agacctgaag gcgatccgca gttatgttgt cggatggcct gctggctgaa taaaaaaacg
    16261 ggaccacgac cagtcccgga accatgagtt ttagggtatt agtttgttat catagttagc
    16321 gtgctaagta tgccatgtca ggttgattag tgaagtgatg ttgttcgcat ttttgcacgg
    16381 cggagaatat tcagattttt ggaaatccat atttttcccg tgcgcgatta catgcttcat
    16441 ttacgatgct attttcgccg gacattgcga attccctgca tgtggacggt ttttgtaaac
    16501 agagcaatat gcgttttctc cgggggggcc ggcaagagcg ccacatcggg gatttttctg
    16561 attggtgccg cgcatacagc gatgaaaagg agatatttgc tcagtgagac tgaccgaaac
    16621 ctttccgcca gcatcgtcag cttctgccca gtaaaaagag acgcggaaaa tgcacaacag
    16681 gcaccacacg tcatgcatgg attcagattg ctcataattc accagtacag ctataaatcg
    16741 taaagagaac agcggtacat cgtatgtaag aacgtaccgc ggctggctgg tgaacttccg
    16801 atagtgcgag tattgaatga tttccagccg ttaccgattt tacgtgctaa ttagtgaaca
    16861 aaccactcgt cagcagactc ccaggtatct ttcagagttt cctgaacaaa agttttagct
    16921 gaatctttat cggcggtgcg cgtaacagaa aggccatcgt tgctggtagc ttttacgatc
    16981 acctctacat cgtcataacg tttactgatg cgtcgggtta attcttcctt taacgcatcc
    17041 acagcaccgg ttggcatttt agtcattttt tctttggcta tgcagatttc aatacgcata
    17101 aaagtccctc tacactgtgt ttgtatacag tatcattttt aactgtatgg ataaacagta
    17161 ttaagggtga aggaaagtga ttttttgggg catgcatggg gcaaaaatat ttgttgtggg
    17221 gcattttggg gcatgattgg gacactttgt tacatatgaa ctttgccgat ttttatatga
    17281 catggaaata caacgctttg attcttctaa ataaaaaaca tgctcttggg cgttctttag
    17341 tgatttttaa aaattccgta tcacgcagtt aaagtggcgg gcatactctt caaggctggt
    17401 gatccccaac cgcacccatt taggatgcga ccattgcggt agaccgatgt aaatcataac
    17461 gcgtgaagga tctcttccac gctacgcaca cgggcgatgc gcgggtagat atgattaatg
    17521 ctgttattgt gttgctcggc gctggcggca ctacaggcgt cttcggcaat caccagatta
    17581 aagccaagtt cccaggcatt gcgggcggtg gattcaacac cgatattggt cgagatccca
    17641 cataacacta ttgtatcgat acctcggcgg cgcagttgca actccagatc cgtaccgtaa
    17701 aacgcacccc actgacgttt gatgatttcg atatcactgt cagttgcacc taatgcagct
    17761 ggatgttgcc accagttttc gggcaacact ttagcggggg
//
LOCUS       DAZPEB010000067        17732 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZPEB010000067 DAZPEB010000000
VERSION     DAZPEB010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17732
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.068"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..224)
                     /locus_tag="ACHURJ_002021"
     CDS             complement(<1..224)
                     /locus_tag="ACHURJ_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HFR1280970.1"
                     /translation="MTWNLLALATALQTVPEQNIDVTNSENALIIKMNDYGDLQINIL
                     FTSRQMIIETFICPVSSISNPDEFNTFLLR"
     gene            complement(351..1082)
                     /gene="rlmB"
                     /locus_tag="ACHURJ_002022"
     CDS             complement(351..1082)
                     /gene="rlmB"
                     /locus_tag="ACHURJ_002022"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFR1280971.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     AQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLVRT
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(1263..3704)
                     /gene="rnr"
                     /locus_tag="ACHURJ_002023"
     CDS             complement(1263..3704)
                     /gene="rnr"
                     /locus_tag="ACHURJ_002023"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFR1280972.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAECRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRLVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3743..4168)
                     /gene="nsrR"
                     /locus_tag="ACHURJ_002024"
     CDS             complement(3743..4168)
                     /gene="nsrR"
                     /locus_tag="ACHURJ_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFR1280973.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4373..5671)
                     /gene="purA"
                     /locus_tag="ACHURJ_002025"
     CDS             complement(4373..5671)
                     /gene="purA"
                     /locus_tag="ACHURJ_002025"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFR1280974.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5775..5972)
                     /locus_tag="ACHURJ_002026"
     CDS             complement(5775..5972)
                     /locus_tag="ACHURJ_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFR1280975.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(6054..7058)
                     /gene="hflC"
                     /locus_tag="ACHURJ_002027"
     CDS             complement(6054..7058)
                     /gene="hflC"
                     /locus_tag="ACHURJ_002027"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFR1280976.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(7061..8320)
                     /gene="hflK"
                     /locus_tag="ACHURJ_002028"
     CDS             complement(7061..8320)
                     /gene="hflK"
                     /locus_tag="ACHURJ_002028"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFR1280977.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTYPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8406..9686)
                     /gene="hflX"
                     /locus_tag="ACHURJ_002029"
     CDS             complement(8406..9686)
                     /gene="hflX"
                     /locus_tag="ACHURJ_002029"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFR1280978.1"
                     /translation="MFDRYDAGEQAVLVHIYFTKDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9763..10071)
                     /gene="hfq"
                     /locus_tag="ACHURJ_002030"
     CDS             complement(9763..10071)
                     /gene="hfq"
                     /locus_tag="ACHURJ_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFR1280979.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(10157..11107)
                     /gene="miaA"
                     /locus_tag="ACHURJ_002031"
     CDS             complement(10157..11107)
                     /gene="miaA"
                     /locus_tag="ACHURJ_002031"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFR1280980.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(11100..12947)
                     /gene="mutL"
                     /locus_tag="ACHURJ_002032"
     CDS             complement(11100..12947)
                     /gene="mutL"
                     /locus_tag="ACHURJ_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFR1280981.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12957..14294)
                     /gene="amiB"
                     /locus_tag="ACHURJ_002033"
     CDS             complement(12957..14294)
                     /gene="amiB"
                     /locus_tag="ACHURJ_002033"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFR1280982.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSNVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKHSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14313..14774)
                     /gene="tsaE"
                     /locus_tag="ACHURJ_002034"
     CDS             complement(14313..14774)
                     /gene="tsaE"
                     /locus_tag="ACHURJ_002034"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFR1280983.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14746..16293)
                     /gene="nnr"
                     /locus_tag="ACHURJ_002035"
     CDS             complement(14746..16293)
                     /gene="nnr"
                     /locus_tag="ACHURJ_002035"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFR1280984.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLFAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLRQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16292..17431
                     /gene="queG"
                     /locus_tag="ACHURJ_002036"
     CDS             16292..17431
                     /gene="queG"
                     /locus_tag="ACHURJ_002036"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFR1280985.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQPICPWNRYSQLTTEEDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17414..17467)
                     /locus_tag="ACHURJ_002037"
     CDS             complement(17414..17467)
                     /locus_tag="ACHURJ_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1280986.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         4079 a         5023 c         4405 g         4225 t
ORIGIN      
        1 tttcttaata agaaggtatt aaattcatct ggattgctga tactacttac cggacaaata
       61 aaggtttcga taatcatttg gcgggaagta aaaagaatat tgatttgcaa atcgccataa
      121 tcattcattt taataattaa tgcgttctcg ctatttgtta catcaatatt ttgttcaggt
      181 acagtttgca gcgccgtcgc cagcgccaac agattccatg tcatatatta ttccatatag
      241 atttagttta aatattaata aaatgaatat ttgcagtacg taattatctt accagctata
      301 gacaaaaaaa aaccatccaa atctggatgg cttttcataa ttcagaggaa ctagctgcgc
      361 tgacgcaccg cttcaaataa gcaaattccg gttgcaaccg aaacgttcag agaagaaaca
      421 cttcctgcca ttgggatgct gatcaactca tcgcaatgtt cacgggtcag gcgacgcata
      481 ccttcacctt ccgcgcccat caccagcgcc aggcgtccgg tcattttgct ctgatagagt
      541 gtatgatccg cctcacctgc cgtaccgacg atccagatat tctcttcctg caacatacgc
      601 atggtacgca ccaggttagt cacccgaatc agcggaacgc tttctgccgc gccacaggcc
      661 acttttttcg ccgtggcgtt gagctgggcg gagcgatctt tcggcacaat caccgcatga
      721 acaccagcgg catctgcgct acgcaggcac gcgccgaggt tatgcggatc ggttacaccg
      781 tcaaggatca gcaggaacgg ctgatcgagc gaagcgatca gatccggcaa atcattttcc
      841 tggtactgac gtcctggctt cacgcgggcg ataatgccct gatgcacggc accgtcgctt
      901 ttctcgtcga gatattggcg gtttgccagc tggataacca cgccctgggc ttcgagggcg
      961 tgaatcagcg gtaacagacg tttatcttca cggcctttta aaataaagac ttcctgaaaa
     1021 cgttcagggg cgcgctccag cagggcctgc actgcgtgga tgccgtaaat catttcgctc
     1081 attaatgtac tcgttgttta cgttttgcct gatgcactac gtttatccgg cctacatgat
     1141 ctctgcaata tattgaattt gcgtgattat gtaggccgga taaggcgttc acgccgcatc
     1201 cggcatgaac aaagcgcatt ttgtcagcaa tctaaccctc ttctgtttga agagggtaat
     1261 gatcactctg ccactttttt cttcgccgca cgcttcgctt tggtcgctgc agctattttc
     1321 tgcgttttcg ccgatggctt tttcgctttt ctcgcgtctt tcttcgctgc tttcggcttc
     1381 gtttttttct caccgcggaa ggcgctgtct ggctcaaagt ttaccttttt accgaccaga
     1441 cgacgcttgc cgcctttttt acctgcatcg ccttttttcg ctttctcgcg cgccgtttta
     1501 ccgacgttgc gcggtgcgcg ttcgctggag atcaggctaa agtcgatttt gcgctcgtcc
     1561 atattaaccg cttcgacgcg aacttccacg cgatcgccca gacgataagt ctggccgctg
     1621 gattcaccca tcagccgttg ccctacctgg tcaaagcgat agtagtcatt gtccagcgaa
     1681 gagacatgga ccagaccatc aatgaataag tcgtccagac ggacaaagaa tccaaagcca
     1741 gtgacgctgg aaattacgcc tttaaagacg ttacctacct ggtcgagcat gaagtcacac
     1801 ttcagccagt cagccacatc gcgcgttgct tcgtcggcac gacgttccgc catcgaacag
     1861 tgctgaccca gttgcagcat ttcttccatc gaataatggt agccgccggt ttcagtggtg
     1921 ttgccctgat gcccctgctc tttcgccagc agatatttaa tggcgcggtg cagcgtcagg
     1981 tctggataac gacgaatcgg cgaagtaaag tgcgcatagg actgcaatgc caggccaaag
     2041 tgaccacggt tttctggatc gtaaatcgcc tgtttcatcg agcgcagcag catggtttgc
     2101 agcatttctg catcaggacg atcggcaacc gactccagca gctccgcgta gtcacgcggt
     2161 tccggcttgt taccgcccgg cagttccagc cccagctccg ccagtactga acggaaagag
     2221 gtaatcgctt cggtgctcgg cttgtcgtga atacggaaca atgctggttc tttggctttc
     2281 tcaacgaaac gcgccgccga gatattcgcc agaatcatgc actcttcaat taacttgtgt
     2341 gcgtcgttac gctgagtctg ttcgatacgc tcaatacggc attcagcgtt gaaaataaac
     2401 ttcgcttctt cgctctcaaa tgagatcccg ccacgttctt cacgggcttt atccagcact
     2461 ttatagaggt tatgcaactc ttcgagatgc ttaaccagcg gagcgtactg ctcgcgcaga
     2521 tcctgatcgc cctgcagaat atgccagact ttggtgtagg tcagacgcgc gtgagagctc
     2581 atcaccgctt cgtagaattt gtagcccgtc aggcggcctt tcgacgaaac cgtcatctcg
     2641 cacaccatac acaggcggtc tacctgcgga ttgagcgagc acagaccgtt agagagcact
     2701 tccggcagca tagggataac ctgcgaaggg aagtacaccg acgtaccacg attacgcgct
     2761 tctctatcca gcggcgttgg cggacgcaca tagtagctga cgtcggcaat cgcgacccat
     2821 aaacgccagc cgccgccgcg ttttttctcg cagtaaaccg catcgtcaaa gtcacgggcg
     2881 tcttcgccat caatggtgac cagcggtaaa tcgcgcaaat cgacacggcc cgcttttgct
     2941 tcttccggca cttcttcttt cagcccggca acctgttgct caacagcctg cggccagatg
     3001 tacggaattt catgggtacg caaagcgata tcaaccgcca tgccggtgcc catattgtcg
     3061 cccagcactt cgacgatttt acccaccgct ttggtacggc gagtcgggcg ttgggtcagt
     3121 tcaacaacca ccacaaagcc catccgcgcg cccatgatct gatcgggcgg gattaagata
     3181 tcgaagctca gacggctatc gtcaggaacc acaaagccga cgcccgcttc ggtaaagtag
     3241 cgaccaacaa tctggctggt ttttggcacc agtacgcgga caatacgcgc ttcacgacga
     3301 cctttacggt cagcacccag cggctgagcc agcacctgat cgccatgaat gcaggttttc
     3361 atctgctcgc tggagagata caaatcatct ttacgccctt caacccgcag aaagccgtag
     3421 ccatcacggt ggccaataac ggtacctttc accaggtcga ggcgttccgg cagcgcatag
     3481 cactgacgac gagtgaagac cagttgacca tcgcgctcca tcgcgcgcag gcggcgacgc
     3541 aggccttcaa gctgctcttc gccttcaatg tgcagttcta ccgccagctc atcacggctg
     3601 gccggttttt cacgtttggt taaatgttcg aggataaatt cccgactagg gatgggattc
     3661 gcgtattttt cagcttcgcg ttcctggaaa ggatcttgtg acatctcggt tcctccgttg
     3721 tcatctctga tgaagatttt cgtcactcca ccagcaataa tttataaagc ggttgattct
     3781 cttcaaccaa atcggcaagc gtgtagttat ccagttccgt aagaaaactt tgcacggcct
     3841 tagaaagcgc ctgtttcaac cgacaggcag gtgtaatgtg gcaaaactca ctgctgcaat
     3901 tcaccagcga taagggctcc agctcgcgca ccacatcacc aatacgtatc gcactcgccg
     3961 gtttacccag gcgaatgccg ccattttttc cacgaacagc agtcacgtag ccggcacgac
     4021 taagttgatt gattattttg accatatgat tacgggagac gccgtagacg tcagtcactt
     4081 cagaaatact ggtcatccgc ccttctggca atgacgccat gtagatcagc gcacgtaatc
     4141 cgtaatcagt gaaactcgtt aactgcacat caacctcaaa agggaaatcg ggaaaaaata
     4201 catttatatt gatgataaac cagccacaag ctgtgtcgct aatttatttc agttcgggga
     4261 aggaaaaaag cgaggattta acactgtgtc ggatagcgta atggcttaac cgacacagca
     4321 atgtatcaca ctgttagccc ggcaggcaaa atatctgcca ggcgtaccag aattacgcgt
     4381 cgaacgggtc gcgcagaatc atagtttcag tacgatccgg accggtagag atgatatcga
     4441 tcggcacacc ggtcagctct tcaatacgct tgatgtagtt cagcgccgcc tgcggcagcc
     4501 cgctacgatc tttcacgccg aaagtggatt cagaccagcc cggcatggtt tcgtaaatcg
     4561 gctctacacc tttccagtcg tcagctgcca gcggagtggt agtcacttcg cgaccatccg
     4621 gcatacggta agccacgcag agtttaacct ctttcaggcc atccagaacg tccagtttag
     4681 tcaggcagaa gccagacagg gagttcagct gtaccgcacg acgaacggca acggtgtcca
     4741 gccagccggt acgacgacga cgacccgtcg ttgcgccgaa ttcgttaccc tgcttgcaga
     4801 ggaactcgcc agtttcatca aacagttcgg tcgggaaagg acctgcaccc acacgagtgg
     4861 agtaagcttt gaggataccc agaacgtaat caacataacg cgggcccagg ccggaaccgg
     4921 tcgccacgcc accagcagtg gtgttggaag aagttacgta cggataagta ccgtggtcga
     4981 tatccagcag cgtaccctgc gcaccttcaa acatgacgaa atcgccacgc tgacgcgcct
     5041 ggtcgagcag gtcagaaacg tcaaccacca tagaagtcag gatgtcggca acagccatcg
     5101 tatcatccag aactttctgg taatcaaccg cttcagcttt gtagtagtta accaactgga
     5161 agttgtgata ttccatcact tctttcagtt tttcagcgaa ggtttctttg tcgaaaaggt
     5221 cgccaacacg cagaccgcga cgtgccactt tatcttcata agcaggcccg ataccacgac
     5281 cggtggtgcc gatcgctttc gcgccacgcg ctttctcacg cgcgttatcc agcgcaacgt
     5341 gataatcaag gatcagcgga catgcttcag acagcagcag acgctcacga acggggatgc
     5401 cacggtcttc cagttctttc atctctttca tcagcgcggc cggagacagc acaacaccgt
     5461 taccgatgat gctggttaca ttctcgcgga gaatacctga tggaataaga tggagaacgg
     5521 ttttttcacc gttgattacg agagtatggc ctgcgttgtg accgccctgg tagcgtacaa
     5581 catatttagc tcgttcagtc agaagatcga cgatcttacc tttaccttcg tcaccccatt
     5641 gggtgcccag tacgacgacg ttgttaccca tttttcaaaa tcaccgtttg cttaaaaatg
     5701 gattctacca tcgctttttc agagttacag cactttttgc actcaaaaat gacctcgatc
     5761 agtctgtttt ttgttcagcc aatcgttttc ctcaacatgt agtagaccac aacgcccgca
     5821 accacaagtc caccgccaaa acgacgtaaa atattatcgg gcaaattggt catcgcagag
     5881 atcatcttct tccatgcctt cgggtaaagc atcggcccta aaccttccag taccaaaacc
     5941 agggcaagcg ccagccagat tgtcgaattc attcattatc cttatagaaa aagaaaacca
     6001 ccgacatccc tgaggatgcg gtggctttat tgacctgtac cgcagtcgtt atattaacgc
     6061 gttgcggaag tcggcgtctt catgtagcgg aagaaatcgc tatccgggct catgaccatc
     6121 acgtcctgat tgccagagaa gctgttctca taagcacgca ggctacggat gaatgcgtag
     6181 aagtccggat ctttactgaa tgcatcagca aacagtttgg ctgcttcggc atcgccttca
     6241 ccacgcatga tgcggccctg acgctcagct tctgccagcg ttctggtcac ttcatagtcg
     6301 gcagtcgcgc gcagtttttc cgcttcttcc tgaccttgtg aacggtgacg acgcgctacc
     6361 gcttcacgct cggcgcgcat acggttgtag atcgcttcag acacttcggt cggcaggttg
     6421 atctgcttga tacgcacatc gacaacttca atacccagcg ccgccatact gttcgggttg
     6481 atgaccggaa ctttgccctt cgtctcagcc gttacgcgct ctgccgcttc ggcaatggcg
     6541 ttatctgctg ccggggtagt aacttcatct tctgtacccg cagaaccgga gttcagcgcg
     6601 tcacgtactt cgagggtcag acgaccacgg gaatcagtga cgatatcttt cacgtccagg
     6661 cgaccaattt cagaacgcag acggtcagag aacttacgtt tcaacagcac ttccgcttgc
     6721 gaaatgtcgc caccacccgt tgccaggtag taacggctga aatcgctgat gcgccatttg
     6781 atgtaagagt cgacgatcag gtctttcttc tctttggtca caaagcggtc ggcctggttg
     6841 tccatggtct gaatacgtgc gtcgagcatt ttcaccgttt caatgaacgg tatcttgaaa
     6901 tgcagacccg gctcataaac cagaggtttg ttgtcatcgt cacgcagtac cttaccaaaa
     6961 cgcagcgtaa taccgcgctc accttctttg acgacaaaga cagacatgta aagcactacc
     7021 agcacgatga tgataatcgc gataactgac ttacgcatcg ttattccccc tgacgctggt
     7081 agtcgttacg ctgcgcgttg gcgcggcgtt ggtccataat atcgccctga ctggtggacg
     7141 acgtgttgct tgctccactg gttgtggagg aagaggctgg cggcaaacgc agcagattgc
     7201 tggcaccgtt atcgctcttc gccgcagggg cgttaccacc tttcagcatc tggtctaacg
     7261 gcagaaccat caggttgcca cctttatcgt taaccagcac tttgcgggtg ttacccaaca
     7321 ctttttccat cgtctcgata tacagacgct cgcgagtaat ttccggcgcg gctttatatt
     7381 ccggcagaag tttagcaaag cgcgccactt caccctgagc ttccaggatg gtctgggcct
     7441 tgtacgcacg cgcctcttcg aggatacgtt gcgcctgacc gttcgcacgc ggctgaactt
     7501 cgttggtata cgcttctgct tcacgaatgt attgctgttc gttttcacgc gcggcaatcg
     7561 catcgtcaaa cgccgctttt acttcttccg gcggacgagc agcctggaag ttgacgtcca
     7621 gcagagtgat gcccatgtca tacggacgaa tcgtctcttc cagttcgcgc tgggtatcgc
     7681 tacgaatcac ggtacgacct tccgtcagaa tgcggtccat ggtgtattta ccgataaccc
     7741 cgcgcagggc gctgtcggta gcctgacgca ggctgtcatc cgggctggtc acgctataca
     7801 gatatttttc cggataggta acgcggtact gcacgttcat ctcaacgcgc actacgttct
     7861 cgtccgacgt cagcatcaca ccggaagcgg ccagttcacg cacggcttcc acgtttaccg
     7921 gtttgacttc gtcgataaac gtcggtttcc agttcagacc cggctcaacc agatggctga
     7981 atttaccaaa gcgtgttacc acgccgcgtt cggcttcttt aatggtatag aaaccactgg
     8041 ccgcccagat aatgacaatc gctgccgctg cgatggtaac gacacgaccg ccaagctgcg
     8101 ggcgcgggcc ttgcgatgaa ctgccaccgc cagatccggt gcctttaccg ccgcccagac
     8161 caccgagctt tttgctcagt ttgcggaaga tatcatctaa atcaggtggc ccttgatcgc
     8221 gaccgccttt gtttccattt ccctcagagt tgccgccagg tttgctgctt ccccacgggt
     8281 cgcggtcttg tccgttatta ccgggctgat tccacgccat gtttgtgctc catatttgtt
     8341 atgcggtgat ccctgttgtc tttcgacgcc agaggatatg actccacgct tcagacgcta
     8401 cgccgttaga tcaggtaatc gatcaacgcc ggttcttgtt tacagaggcg acgccagtca
     8461 acgatcggca tacgaacttg cagacttacg ctgccgtcct cctccatcca ctctttttct
     8521 attgcctgaa gctgataaaa acgacttctc agacgccctt cctgcggtgg cagacgcaat
     8581 gtatgctgcg ccacctcgcc ggaaagccgc tccgtcaaag cctgaaaaag ctgtggtatc
     8641 cccgctccgg tctgtgcgga aagccagaca cggatcggtt tgttctcttc gtcccgatca
     8701 atacgcggtt cgaaatcttc cagcatatcg atcttgttca tcaccagcag ggttgggatc
     8761 tcgtgagcgt cgatctcttc aagaaccgta ttcaccgctt cgatgttttc ttgtacacgc
     8821 acatccgccg catcaatgac gtgcagcagt aatgtggctt gccgcgtctc ttgtaacgtg
     8881 gctttaaatg ccgccaccag atcgtgcggc agatggcgaa taaaccctac ggtatctgca
     8941 agtacggttt caccgacatc cgcgacgtca atacgccgca acgtcgggtc gagggtggca
     9001 aacaactggt ctgccgcgta gacccgcgct tcggtgatgc gattgaaaag ggtagattta
     9061 ccggcgttgg tatatcccac cagcgaaaca gtaggaacgt cggctttgat acgcgattgc
     9121 cgcccctgct cacgctgctt ttcaactctt tccaggcgcg actgtatctg cacgatgcga
     9181 ttacgcaaca aacgacggtc ggtttcgagc tgggtttcac ccggaccacg caaacctatc
     9241 ccgcctttct gtctttcaag gtgggtccag ccacgcacca ggcgcgtagc cagatggcgc
     9301 agctgcgcca gctcaacctg caacttaccc tcatgggtac gcgcacgttg ggcgaaaata
     9361 tctaaaataa ggccggtgcg gtcgatgaca cgacactcgc acaaacgctc caggttacgc
     9421 tcttgcgccg ggctcagggc atggtcaaaa agaacgaccg aagcacccgt cgctttgaca
     9481 gcttccgcaa tttcaactgc tttaccttca cctacaaaat actttgggtg cggcgcttta
     9541 cggctaccgg taatcacctg caatgcttcg acaccggcgg aagaaaccag agattcaaac
     9601 tcctggaggt cttccatatc tttgtctttc gtaaaataga tgtgtaccag taccgcctgc
     9661 tcaccagcat cataacggtc aaacaagcgt ataaccctct aaatagatca gcggggaacg
     9721 caggatcgct ggctccccgt gtaaaaaaaa cagcccgaaa ccttattcgg tttcttcgct
     9781 gtcctgttgc gcggaagtat tctgcgcgct gctaccatga tggtagttac tgctggtacc
     9841 gccaccggcg ttgttactgt gatgagaaac cggacgagac gggacaacag tagaaatcgc
     9901 gtgcttgtaa accatctggc tgaccgtgtt tttcaacagg atcacgaact gatcaaaaga
     9961 ctcgatttgc ccttgcagct taataccatt caccaaataa atagaaactg gaacacgttc
    10021 ccgacgcagt gcgttcagga acggatcttg taaagattgc cccttagcca ttctctcttt
    10081 tccttatatg cttatttgta ctttgaacct ttcgattctg aaaaaattgc gcacgatacg
    10141 tctcaattgt acacattcag cctgcgatag caccaacaac ctgtaatact tcgtcacgcg
    10201 cctgttctgg tttttcactg tcaagccagt gaaccccttc ccaaccacgc agccaggtta
    10261 tctgccgctt cgccaactgt ctcgtggcgc aaacacctcg ataaaccatt tcatcgtatg
    10321 agatttcgcc ttcaaggtaa gaccacatct ggcgataacc cacgcaacga atggaaggca
    10381 agtccgtatg caaatctcct cgggcaaaaa gcgcccggac ttctgcttca aaacctgaag
    10441 ccaacatctg atgaaaacgc tgctcaatgc gttgatggag cagttcacgg ctcgccgggg
    10501 cgatggcgaa ctgatgcacc tgatacggta gagcgtctcc tgacgtttgc gtcagttccg
    10561 ttaaagtttt acccgaaatg aaaaaaactt ccagtgcccg ggaaagcctt tgtggatcat
    10621 ttggatgaat ccttgctgcc gcaaccggat ctacctcctg aagttgacga tgcaatgact
    10681 cccaaccttg ctctgccgcc tgttgctcaa ttctggcccg cacttccggg tctgccgacg
    10741 gtagcggcga caacccttcc agcaatgcct tgaaatacaa catcgtaccg cccactaaca
    10801 gtgggatccg ccccgccgcg gtgatatcgg ccatttccgc cagcgcatcg cggcgaaaat
    10861 cagcagccga gtaagcctgc gacggatcgc gaatatccag caatcggtgc ggcgcggcga
    10921 gtaactcttc agcgttcggc ttcgccgtcc cgatatccat ccctttgtaa ataagggcag
    10981 aatcaacgct tatcaactct actggtaaaa ttttacgcag ctcaatggct aacgccgttt
    11041 taccggaggc cgtcggcccc atcaaaaaaa tcgccttagg caggctcgcc ttactgatat
    11101 cactcatctt tcagggcttt tatcgccgga tgtaaatcaa cagattgtaa cagaccaccc
    11161 ggcggcgttt tcacaagttg cggacataac cgttccacgt ccgccagcag ggttatggcc
    11221 tgtgccattg accactgcgc atgttcgctc atcagatttc gtgcaatcca ctgcgcaata
    11281 ttgccaggtt cgaatacgga ctgcttcgcc aggtagccta tcagttcagg aatcaagatt
    11341 tgtaaatttt gttggcgtaa gggtaaaggc acggccctga tggtcacatg ctgtgcatct
    11401 gactggaaat caatacccaa ttccgccagg gcagactgcg ctttttctaa tgccgatttt
    11461 tcttcgccag aaacttttag ccgcaacgga ataagcagcg gctgagcgca aactggcgct
    11521 tcacccgggg tcagttgtac ctgacgcagc caacgttctg ccactggcaa ggctaaaagt
    11581 gaaatgttgc cgtcgcgctc cagcaacgca cagtcggaat ggacgatagt cagtacccga
    11641 ccaaaactct gactgttcgc cgcaagtgca ggttcctgcg gttccggcgc ttttaatttt
    11701 tgcatcggcg cgggcgtttg caaaagctgg cgatacactt caccttgctg tttctggtag
    11761 cctggctgcg cattcggcca aggggcagcc ggacgactgc ctgatgctgg cgcaggagtg
    11821 tagcgcggag ctaccggctc acgagctgcc ggttctgcaa agtgattgcg ccccgccgcc
    11881 acgcggtttt ccggaatgga acgcggtgca ggttggggtt catcgtccag cggtagcggc
    11941 gtttccagtt gctgttgtag cacgctcagc acgccctgat agataaaatc atgcaccaga
    12001 cgcgactgat ggaaacgcac ttcgtgtttg gcggggtgca cgttgacgtc cacctgatgc
    12061 ggatcgatct ccagatacag cacaaatgcc ggttgctgat cggcccccag tttgtcttcg
    12121 caggcctggc ggatcgcgtg attgatcagg cgatcgcgca tcatgcgacc gttcacgtag
    12181 caatactgaa tttctgccag tgcgggcgtg gtgtgatttg gatcggccac ccagccgcgt
    12241 agcgtgagat cgccgtgttg ccattcaatc gccagcgctt gttcaagaaa agcggtgccg
    12301 caaatcgcgc ctaagcgccg ttctttttgc ccgccttccg gcactgcgcg gtactgacgc
    12361 acaattttac cgttatgcga caggttgatc gtgacgtcga aacgcgccag cgcaatgcgg
    12421 cggatgatct catcaatgtg gttaaattcg gttttctcgg tgcgcaggaa tttgcgccgc
    12481 gccggggtgt tgtagaacag atccagcacc tccagcgtcg tccccacagg atgcgccgcc
    12541 ggttttaccg tcacgttcat atcgcgccct tcggcatagg cctgccaggc ttcctgctgt
    12601 tctgcggtgc gtgaagtgag cgtcaggcgg gaaaccgaac tgatactcgc cagcgcctca
    12661 ccgcgaaagc ccaggctgat aatggcttcg agatcgtcca gagaggcgat tttactggtg
    12721 gcatgacgag ccagcgccag cgccagctca tcttttttga taccgcagcc gttatcacga
    12781 atgcggataa gtttcgcccc accgcgttcg atatcaatat cgatacgcgt cgcacctgca
    12841 tcgaggctgt tttccaccag ttctttgact accgacgcag gtcgctcgac cacctcacct
    12901 gcggcaatct ggttcgccag ttgtggcggt aagacctgaa ttggcatcaa tcgtccttag
    12961 tttggcagcg tgcgatctgg cgtcgtcacc gtactggccg tttgtgccgt tgcaccctgc
    13021 ggcgcagatt gcatcggatg cgcaaggaaa taattgcgca ggcctttgta aatggcttct
    13081 gccagctgtt gttggtaatc gtcgctcgcc agcaaacgtt cttcgctgtt gttgctgata
    13141 aaaccggttt cgaccagtac tgacgggata tccggcgaac gcagaacgcc gagactcgcg
    13201 tgttctggtc ggcgtttatg tatttcgcca atgcgttgca actgactgat catactggtc
    13261 gctacatcat accctacccg ctgggaatga ccgaactgta aatccagcac cgcctggctt
    13321 aaatagggat cagactgact gttcgccagc acatcgcccg ccccacccag cagctccgaa
    13381 tgtttctcgt gctgctccag ccagctggcc atttcgctgt tggcgcgacg gttagagagc
    13441 acccatacgg aagcgccagt cgcactgcgg ttcggtgcgg catcagcgtg aatcgacacg
    13501 aggaaattag cgttttgctt acgtgccaca ttgctgcgcc ccatcaccga aataaagtaa
    13561 tccccgtcac gggttaaaac gcctttaaac atcggatcgt cattgagcaa agtacgcaat
    13621 ttacgcgcga tggcgatggt gacatttttc tcccgcgtac caccggggcc gatagcgcca
    13681 gggtcctgac cgccgtgtcc ggcatcaata gcgatgataa ttttatcgcc agtgttagcc
    13741 gtcgcgcgcg ctgccggacg cgttaccgta ttactgctga taacacccgt agtgcggtta
    13801 ctttccgttt taaacggatt gcgcgccggt tcgctgacgc gcggtgcgac aaccgcaggc
    13861 gtttcaacgc gtttcgcaac cacgggcggc ggaggaggcg gtggcggcac atcggcgtta
    13921 atcgtaaaga cgacagtgta attgctgcca ttctgccgct tcaccgcttc ggttttacca
    13981 ttttcggtaa gatcgaccac cagccgtagc gtttgtgcat ctttaggcgt tccagagcga
    14041 atcgccttca ccagattatt gccgctgaac aacaacggca gtccctgaat cacgcccgtt
    14101 tgtttgatat cgagcgccac ggtgcgtttg ctttgatggc taaacgcata atcaggatcg
    14161 ccaataaaac tcaacgttat ccgcgcctgt tgattaccgt tagaaacctg aatatcagag
    14221 agcgtcgcgg cacccaccgg cgtgcacagc agcagcagcg tcgctaccaa ccaatttctg
    14281 atgcgataca tcatcccgcc acctttcaaa ggttaaccgg ctaaacgcgc cagcaacaat
    14341 tcacccgcag aggaaaccgc actcacgcgc gcctcacggc cttgtgcctg ataatcaatg
    14401 tgtatttcga catccgggtc aggaagaaca cctgtacctt gttgtggcca ctccaccagg
    14461 cagatggcat cgttggcaaa ataatcgcgg atccccataa actccagctc ctcgggatcg
    14521 gcaaggcggt acaaatcaaa gtgataaacc attaaattgt cgagcgtata gggttcgacc
    14581 agcgtataag tggggctttt gacattaccc tgatgaccca gagcttgtaa aaagccccgg
    14641 ctaaaggtgg tcttacctgc gcctaaatcg ccatacagat agattacggt tgcgccatcg
    14701 caggctttcg ctacccgctc gcccaggtct aatgttgcct gctcatcagg gagcggaatt
    14761 actcgattca tcatggtttt tatcagtcac ttccgggtta acaatacgct gtagcgtgga
    14821 aaagagatcg gttgccagca tcccgcgcgt tccaaaacgc gccgccagta cgtcagctgc
    14881 cgcaccgtgc gcgacacagc ccgcacaggc ggcatcatac ggcgacagtt tttgcccaag
    14941 caatgcgcca ataataccag agagcacatc gcccatgccg ccgctcgcca tgcctgcatt
    15001 tccggcatca ataatgccta aagcgtcagg atgggcggcg accacggttc cggcaccttt
    15061 cagcaccgct acgccgccat aacgttgtac cagacgtttg gcgcaatgta agcggtcact
    15121 ttcaatttca gcgacggaac agcctaacaa ccgtgcggcc tcgccaggat gcggcgtgat
    15181 cacgcgattg tgacgcttat cgggattgat tgccagcagg ttcaatgcat cggcatccca
    15241 caacatcggt ttgcgaaaat tctcaacttt ttgcagtgct tttttccccc actcttgctg
    15301 gcccagaccg ggaccaatca ccaccacatc ggcccattcc aggctttcgg taagagagtc
    15361 catcgtcagt tcatgcacca tcaattccgg tcgtgcagtc agcagcggcg caatgttttc
    15421 actgcgggtc agtactcgga ctaaaccagc accagcacgc agcgccgctt cccccgtcat
    15481 acgaatagcc cccgccgtgc cgtgatcgcc accgataatt accagccgcc cgtgatcgcc
    15541 tttatgcgaa gtcgggcgac gcggttttag ccagtgagaa agttgttctg ctgaaaaccg
    15601 ctgaattttc gtctcctgac ctgccagcca actatccagc cccagtgagt caaaatgcag
    15661 ttgtccggta acatcccgcg cttttccagt gagcaagcct ggtttcagcg caataaaagt
    15721 gatggtgtga tcggcgttga tcaccgcgcc tggcgtagcg ccagtttcag ccagcaggcc
    15781 ggaagggata tcaaccgcca caatcggcgc aggatgggaa ttagcgtggt cgattaactg
    15841 gctaatggat tcgcggggcg cttgccgcaa accggtaccg agcagcgcat caacaatcag
    15901 atctaccgat tcgggccaga caatattcga agcatggatc tcgccacccg cgtttaacca
    15961 tgcttcgcgt gccagcgcgg cctcttccgg caacggtttg tcgctctcct gggcaaacaa
    16021 cgtgacctca atgccgaccg ctttggccag tcgcgcgacc acgtagccat cgccgccgtt
    16081 attaccatga ccgcacagca ccagccagtg gcgggcgtca ggatacgccg aacgacacac
    16141 ctggaatgcg gcctcgccag cgcgaagcat cagctcatag agtgtgagcc ccagcacatc
    16201 tgccgcctcg cgttctccgc ggcggatatc gtcggcgtac cagacggtgt gtggtatact
    16261 tacggggttt ttcttcattg tatggtccgt catgtcagag cccctcgatc tcaatcagtt
    16321 agcgcaaaaa attaaacagt gggggctgga actgggcttt cagcaggtag gtattaccga
    16381 taccgatctc agcgagtccg agcccaaact gcaagcatgg ctggacaaac aataccacgg
    16441 cgaaatggac tggatggcac gtcacggtat gctgcgcgct cgccctcatg agttattgcc
    16501 cggtacgctg cgcgtgatca gcgtgcggat gaattacctt cctgctaacg ccgcatttgc
    16561 cagcacgctg aaaaacccca aactcggcta tgttagccgt tatgcgctgg gccgtgacta
    16621 tcacaaactt ctgcgcaacc gactcaaaaa gctgggcgag atgattcagc aacattgtgt
    16681 ttcgctgaat tttagaccgt ttgtcgattc tgcgcctatt ctcgagcgcc cgttagctga
    16741 aaaagctggg ctcggctgga caggtaagca ctcacttatc ctcaatcgcg aggccggttc
    16801 gttcttcttt ttaggcgaat tgctggtcga tattccgctg cccgtggatc aaccagtcga
    16861 ggaaggatgc ggtaaatgcg tggcctgtat gacgatttgc ccgaccggtg ccatcgtcga
    16921 gccatatacc gtcgatgctc gccgctgtat ctcttatctc accatcgaac ttgaaggggc
    16981 gatcccggaa gagttgcgac cgttaatggg aaaccgtatt tacggttgcg atgactgcca
    17041 gcctatctgc ccgtggaatc gctattcaca actcaccaca gaagaggatt tcagcccgcg
    17101 taagccgcta cacgcaccgg aactcattga gttattcgcc tggagcgaag agaagttttt
    17161 aaaagtcacg gaaggatcgg cgattcgtcg aattggtcac ctgcgttggc tgcgtaatat
    17221 cgccgtagca ttaggcaatg ccccttggga tgaaacgatt ttgacagcgc tggaaagtcg
    17281 taaaggtgag cacccacttc tcgatgagca catagcgtgg gcgattgcgc agcaaataga
    17341 gagacgaaat gcgtgcatag tcgaagtgca attgccgaaa aaacagcgtc tggttcgggt
    17401 gattgaaaaa gggttaccgc gtgacgcctg attcatccac agcttgtgaa taaaaataaa
    17461 aacgcattgc aattcaagac gaataaaatc gtcaagtgat cgactcgaca aaacggaatc
    17521 ttaattttta acttatctat caataggtta aaaaaatact attcaccgtg cgaagtttct
    17581 ttgaaattaa gattacagcc tgaggctgtg gataactctg tttacaagag tttgtcagag
    17641 acaacgaaaa ccagcaataa cgcatcgctg tggatatttt attgagagaa gaatttggag
    17701 cgggaaacga gactcgaact cgcgaccccg ac
//
LOCUS       DAZPEB010000068        17699 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZPEB010000068 DAZPEB010000000
VERSION     DAZPEB010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17699
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.069"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(1..610)
                     /locus_tag="ACHURJ_002038"
                     /pseudo
     CDS             complement(1..610)
                     /locus_tag="ACHURJ_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
     gene            complement(789..2147)
                     /gene="plaP"
                     /locus_tag="ACHURJ_002039"
     CDS             complement(789..2147)
                     /gene="plaP"
                     /locus_tag="ACHURJ_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFR1280987.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(2137..2199)
                     /gene="yoeI"
                     /locus_tag="ACHURJ_002040"
     CDS             complement(2137..2199)
                     /gene="yoeI"
                     /locus_tag="ACHURJ_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFR1280988.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(2414..3343)
                     /locus_tag="ACHURJ_002041"
     CDS             complement(2414..3343)
                     /locus_tag="ACHURJ_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR1280989.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLALEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQHFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(3389..4213)
                     /locus_tag="ACHURJ_002042"
     CDS             complement(3389..4213)
                     /locus_tag="ACHURJ_002042"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR1280990.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGSGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGMVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(4296..4550)
                     /gene="yoeB"
                     /locus_tag="ACHURJ_002043"
                     /pseudo
     CDS             complement(4296..4550)
                     /gene="yoeB"
                     /locus_tag="ACHURJ_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
     gene            complement(4547..4798)
                     /gene="yefM"
                     /locus_tag="ACHURJ_002044"
     CDS             complement(4547..4798)
                     /gene="yefM"
                     /locus_tag="ACHURJ_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFR1280991.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            5081..5131
                     /gene="hisL"
                     /locus_tag="ACHURJ_002045"
     CDS             5081..5131
                     /gene="hisL"
                     /locus_tag="ACHURJ_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFR1280992.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    5107..5230
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            5277..6176
                     /gene="hisG"
                     /locus_tag="ACHURJ_002046"
     CDS             5277..6176
                     /gene="hisG"
                     /locus_tag="ACHURJ_002046"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFR1280993.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            6182..7486
                     /gene="hisD"
                     /locus_tag="ACHURJ_002047"
     CDS             6182..7486
                     /gene="hisD"
                     /locus_tag="ACHURJ_002047"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFR1280994.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDEALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPAAD
                     MARRVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSLESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            7483..8553
                     /gene="hisC"
                     /locus_tag="ACHURJ_002048"
     CDS             7483..8553
                     /gene="hisC"
                     /locus_tag="ACHURJ_002048"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFR1280995.1"
                     /translation="MSIVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYGVSAETIGVECRTVPTLDNWQLDLQGISDKLEGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     ECLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            8553..9620
                     /gene="hisB"
                     /locus_tag="ACHURJ_002049"
     CDS             8553..9620
                     /gene="hisB"
                     /locus_tag="ACHURJ_002049"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFR1280996.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFLQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            9620..10210
                     /gene="hisH"
                     /locus_tag="ACHURJ_002050"
     CDS             9620..10210
                     /gene="hisH"
                     /locus_tag="ACHURJ_002050"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFR1280997.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            10210..10947
                     /gene="hisA"
                     /locus_tag="ACHURJ_002051"
     CDS             10210..10947
                     /gene="hisA"
                     /locus_tag="ACHURJ_002051"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFR1280998.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            10929..11705
                     /gene="hisF"
                     /locus_tag="ACHURJ_002052"
     CDS             10929..11705
                     /gene="hisF"
                     /locus_tag="ACHURJ_002052"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFR1280999.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDGETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            11699..12310
                     /gene="hisIE"
                     /locus_tag="ACHURJ_002053"
     CDS             11699..12310
                     /gene="hisIE"
                     /locus_tag="ACHURJ_002053"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFR1281000.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(12407..13384)
                     /gene="wzzB"
                     /locus_tag="ACHURJ_002054"
     CDS             complement(12407..13384)
                     /gene="wzzB"
                     /locus_tag="ACHURJ_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFR1281001.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDFLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGPAVPKVSDIQTSLIGRYSTA
                     FSALAETLDNQEEPEKLTIEPTVKNQSLPLAVSYVGETAEGAQKQLAQYIQQVDDQVN
                     EELEKDLKDNIALRMKNLQDSLKTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSSNYYQTRQNLLDIESLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            13793..14797
                     /locus_tag="ACHURJ_002055"
     CDS             13793..14797
                     /locus_tag="ACHURJ_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010432020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HFR1281002.1"
                     /translation="MKFLVTGAAGFIGFYVSKRLLAAGHQVVGIDNLNDYYDVNLKQA
                     RLDLLKHDNFSFYKIDLADREKMAALFADERFDRVINLAAQAGVRYSLENPNAYADAN
                     LIGFLNILEGCRHNNVQHLLYASSSSVYGMNRKMPFSTDDSVDHPVSLYAATKKANEL
                     MAHTYSHLYGLPTTGLRFFTVYGPWGRPDMALFKFTKAMLEGKSIDVYNFGKMKRDFT
                     YIDDIAEAIIRLQDVIPEKDPQWTVETGSPATSSAPYRVYNIGNSSPVELMDYINALE
                     EALGIEANKNMMPLQPGDVLETSADTKALYDVIGFKPETSVKEGVKNFVEWYRNFYKV
                     "
     gene            complement(14863..16029)
                     /gene="ugd"
                     /locus_tag="ACHURJ_002056"
     CDS             complement(14863..16029)
                     /gene="ugd"
                     /locus_tag="ACHURJ_002056"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFR1281003.1"
                     /translation="MKITISGTGYVGLSNGILIAQNHEVVALDIVQAKVDMLNKKQSP
                     IVDKEIEEYLATKDLNFRATTDKYDAYKNADYVIIATPTDYDPKTNYFNTSSVEAVIR
                     DVTEINPNAVMIIKSTIPVGFTESIKERFGIENVIFSPEFLREGKALYDNLHPSRIVI
                     GEQSDRAERFAALLQEGAIKQDIPILFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNSRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADSILARKPKVVGVYRLIMKSGSDNFRASSIQGIMKRIKAKGVPVIV
                     YEPAMKEDDFFRSRVVRDLDAFKQEADVIISNRMSADLADVADKVYTRDLFGND"
     gene            complement(16278..17684)
                     /gene="gndA"
                     /locus_tag="ACHURJ_002057"
     CDS             complement(16278..17684)
                     /gene="gndA"
                     /locus_tag="ACHURJ_002057"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFR1281004.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGNKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKDGVFHTEWLD"
BASE COUNT         4584 a         4496 c         4351 g         4268 t
ORIGIN      
        1 cagtgacaaa gggatgccag cgtttttcaa acagaatatg agcgatcccg gttcggcgcg
       61 gcggtaaggt cgcgactttg agttttggct tcttcaactc tttcatcacc acccagagcg
      121 ttatcaccag caaaacggca accaacggcc acacagacaa attaaaagtc tcagcaatag
      181 agttatgttc agtactgtag tgctgcaagg tttgatttaa accactggca tgtggagaac
      241 gcatcaccgc actcatcacc atataagtga aaagcgcgat ccaactgccg atcaatccct
      301 cacccgcgcg ataccaggtc ccggtggcac atccacccgc cagaacaatt cccagcccga
      361 agatataccc acctataaca gtaccaggcc acgggaacgc cccggcttcg taagtcagta
      421 ggcccgccta aattaacgca aaaaccccca cactttgtac cgaaattgca atcagcaagg
      481 cgtaaaacat gcgattattt ttcgcgatat acatatcgcg aaaaccacca gtcaggcaaa
      541 aacgcccacg ctgcatgaca aatcccagca gagcaccaca aattagcccg cttaatatca
      601 ttgaaaacat aaattaatta accagatgaa tgttaatgag gaaattattc atgactggtg
      661 gaatgcagac caataaccaa attctctaaa ttagaacaaa tggttcttaa tgaggatttc
      721 gataaaaaag aataaaaaaa ccggagccaa atattcattc gactccggtt gattattaga
      781 aaagatgatt atgctacgtc ttcgtactgc ggaaccgggt tacggaagct cttggtgacg
      841 caagccaggt aaatcagacc gatagcagcc cagatcagac ccagaaccat tgagctttct
      901 tccaggttaa cccacagcgc accaacggtc agcgcaccac acatcggcag gaacagatac
      961 tggaagtgat ctttcagcgt cttattacgc ttctcacgga tccagaactg cgagatgacc
     1021 gacaggttaa cgaaggtgaa cgccaccagc gcaccaaagt taatcagcgc cgttgccatt
     1081 accaggtcga agttgattgc cagcagggca atcgcgccaa ccaggatgat gttcatcgcc
     1141 ggagtacgcc atttcgggtg tacataaccg aagaagcttt tcgggaatac gccgtcacga
     1201 cccattacgt acatcagacg cgctacgcct gcatgcgccg ccataccgga cgccagtacg
     1261 gtaatggtgg agaagatcag cgcgccaacc tggaacgctt tacccgcaac gtacagcatg
     1321 atttcaggct gtgacgcatc cggatcttta aagcgagaga tatccgggaa gtacagctgc
     1381 aggaagtaag ttgcaaagat gaagatcatg ccgccgatca gcgcggtcag gaaaatcgca
     1441 cgcgggatca cgcgctctgc atctttggtt tcttccgaca ggttgctgat gccgtcaaag
     1501 ccggtaaagg agaagcacag gattgtcgcc ccggtaatca tcgggataac atgcgcatcg
     1561 ccagaccaga atggacgagt gctcgccagc gtaccagcgc cttcaccttc aaatacgcca
     1621 taaacaacca tgcccagaat caccgcgatc agcaccacct gcaacacgac gattacagtg
     1681 ttgaagttcg ctacggattt cagactacgc aggttaaagg cggtcatgaa ggccaccaac
     1741 gccaccacaa acatccacga tgggatggaa ggcaccagag cttcaaaata gattttcgcc
     1801 agcagaatgt tgatcatcgg cgcgaacaga tagtcgagca gagaagacca acccaccata
     1861 aagccgacag tcgggctaat ggatttctgg gcgtaagtgt atgcagagcc agcagaagga
     1921 tagcggcgaa ccagcttccc gtagctcaga gccgtaaaca ggatcgcaat caatgcgaac
     1981 gcataggctg tcggcacatg accatccgtg aggcctgaaa cgataccaaa agtatcaaac
     2041 agcgtcatcg gctgcatata ggcaagaccc atcattacaa ccggaactaa cgtaagcgtt
     2101 ttacgcaatt ccacgcgaga ggtgtttgga gtaacgttat gcgacatggt cattctcctt
     2161 tacggtgata accgtcgcgt aagcaaaaaa ttgccccatt tttttggatt cctcagcgac
     2221 aacaactgtc gatttttagt aaatatctat ccggtacgaa gcccggcctc ttggtatgaa
     2281 tgattggttt gaagcaaaaa ataaccgacg ctgatgaaac gtcggttttt agtcattttt
     2341 tgacagcggc gcattgtgcc taaatggggg ggaaatgaca agagaatgag aggcttgtca
     2401 taataatttt tctttaaatg gctgattttc cgtcatcaga ttttgcataa acaccggcga
     2461 tcgcactatt tgctaaaatt tcatcccgcc accagccact ggcaagtcct gcggtctgtt
     2521 cattccagcc aatccagacg ggttcaaaga gagtttgggc gaccactttc ttctcgatta
     2581 acgcgccact atcgagaaaa tgttgcgcca gataacgggg taaatagccg cagccgaggc
     2641 cgctaatttg cagctccagc ttggttttaa aatcgaaaac ggtaatggct tcttgttcgt
     2701 cgagaagttg tgaggccgta gaagcgcctg cctgcgcagt atcccccacc acaatcgccc
     2761 gatatcgctt aataatacga cgatttaacg gctcttcttc cagggctaac ggatgatgag
     2821 gcgcgaccgc aaagacttgt tccagatcgc ccaaccgtga aaaaccaaac tcactggaag
     2881 atggtggctc atgcatcgcg ccaacaataa tatccgctcg cccctgagtc agggcatccc
     2941 atgaaccacc cagtacgcca ttgataaact tcaggcgcgt cacgctatga tgttgataaa
     3001 aagcttcaat cagtggcgct agcagagaaa aaggaaacgt gtcgtccacg ccaatcacca
     3061 gctcgttttc ccagccttca tgcagtttga tcgcctgctt ttccagttcc cgcacggtat
     3121 gcagaacttc ccgccctttc tctaataaca tttttccggt gcgggtgaat ttagcgcggt
     3181 gaccgctacg atcaagcaat tgaatattaa ggtcgctttc cagcttgtga acggtgtaac
     3241 tcagagcgga cggcgtcttg tagagtttgg ccgacgccgc cgcaaaactg ccttcttttt
     3301 ctaaggcatc gaggatcatc aacacatcca gcagtggttt catacacgcc cccttgcagt
     3361 gtatgcggcg tcctgctggc gtgatcgttt actccagcgg cattaccagc ggatcgggat
     3421 actggtattc aaatcccagt tcattacaaa tccgactgcc atcaataatc ttacctttgc
     3481 cgctgtccag actatttctg aactgcggag gttccagccc cagtaaacgg gccatctgcg
     3541 gatagaaaac attacgcgca gggtgagctg gcgcacatat attatagatg tgtccgcctt
     3601 taggtgcctg taacaacaga gtgatagcgc caataacatc ttctaaatgg actaaattaa
     3661 caccatgttc accatcaggc gcggtttttc cggcaaagaa gcgtccggga tgacgccccg
     3721 gtccgaccag gcccgcaaga cgcagaatat cgaccgaagt accgggtaaa ttgtgcagcc
     3781 agtcttcgag ttcttctaat actcgtccac tgttggttac cggattacgc ggggtagttt
     3841 ctttcaccat gccttgcgca tcgccataga cagatgttga gctggtaaaa ataattcggg
     3901 gaatacgatg ggccagcgcg ctatccacta actcttgtac cgcttgtaaa tagaactcat
     3961 cgccggagcc gctacgacgt gccggaagcg taatgaccag tgcatcggca tccatcaggg
     4021 catccagatc gtcagaatcg caaactaact caggctccat gcgaagcaga tagctatcaa
     4081 tgccactcat tcgggccgct tcgacaccat cttgtgtggt tttactcccg gtgacttgcc
     4141 agcctcgcgc tgaaagtgac atcgccagcg gcatgcctaa ccaccctaac ccgacaattg
     4201 cgaccttttt catccgttat ctcctgatac accttagatc tataaggcta cgctagcgta
     4261 tcaaaactga caattcattc tatgaatgaa tctgttcaat aatgataacg acatgctgca
     4321 atgagcagtg aatcgtcggt aaccgcgtat accagacggt gctcctctgt aatgcgtcgg
     4381 gactagaaac ctgacaaatt atgtttcagg ggttctggct tccccttacc ttcaaatggc
     4441 gttctgcggg tatctttgat aagttcattg atctttttaa caattcgctt atctgtttcc
     4501 tgccagtaca gataatcatc ccatgattcc tcagaccaga ttagtttcac tcaataatgt
     4561 ccttttctgt tcctttgcct gatttcaggc tatcgattga gtccatcaat ctccgggcgt
     4621 tagcggggga acgcagtaga taagccgtct cttccagcga gttgtattct tcgagtgaca
     4681 tcagaacaca agcctcccca ttctgacgag taataaggat cggggcatga tcttcaacgg
     4741 ctttcatcat tgttgccgac aaattctgac gcgcttcgct gtagctaatt gtacgcatgt
     4801 caatctcctc ttttgtacag ttcattgtac aatgatgggc gttaattaac tatttattaa
     4861 ttagtttgta gatcaaggta ttgtcagtga gacgaaaatc caggcttcgc tatttttggt
     4921 gccgtcagct tagaggacag tcctcttagc cccctccttt ccccgctcat tcattaaaca
     4981 aatccatcgc catagaatat ataaaaaagc ccttgctttc taacgtgaaa gtggtttagg
     5041 ttaaaagaca tcagttgaat aaacattcac agagactttt atgacacgcg ttcaatttaa
     5101 acaccaccat catcaccatc atcctgacta gtctttcagg cgatgtgtgc tggaagacat
     5161 tcagatcttc cagtggtgca tgaacgcatg agaaagcccc cggaagatca ccttccgggg
     5221 gcttttttat tgcgcggttg ataacggttc agacaggttt aaagaggaat aagaaaatga
     5281 cagacaacac tcgtttacgc atagctatgc agaaatccgg ccgtttaagt gatgactcac
     5341 gcgaattgct ggcgcgctgt ggcattaaaa tcaatcttca cacccagcgc ctgatcgcga
     5401 tggcagaaaa catgccgatt gatattctgc gcgtgcgtga cgacgacatt cccggtctgg
     5461 taatggacgg cgtggttgac ctcgggatta tcggcgaaaa cgtgctggaa gaagagctgc
     5521 ttaaccgccg cgcccagggt gaagatccac gctactttac cctgcgtcgt ctggatttcg
     5581 gcggctgccg actttcactg gcaacgccgg ttgatgaagc ctgggatggc ccgctctcct
     5641 taaacggtaa acgtatcgcc acctcttatc ctcacctgct caagcgttat ctcgaccaga
     5701 aaggtatctc ttttaaatcc tgcttactga acggttctgt tgaagtcgcc ccgcgtgccg
     5761 gactggcgga tgcgatttgc gatctggttt ccaccggtgc cacgctggaa gctaacggcc
     5821 tgcgcgaagt cgaagttatc tatcgctcga aagcctgcct gatacaacgc gatggcgaaa
     5881 tggaagaatc caaacagcaa ctgatcgaca aactgctgac ccgtattcag ggtgttatcc
     5941 aggcgcgtga atcaaaatac atcatgatgc acgcaccgac cgaacgtctg gatgaagtca
     6001 tcgccctgct gccaggtgcc gaacgcccaa ctattctacc gctggcgggt gatcaacagc
     6061 gcgtagcgat gcacatggtc agtagcgaaa ccctgttctg ggaaacgatg gaaaaactga
     6121 aagcgctggg tgccagttca atcctggtcc tgccgattga gaagatgatg gagtgatcgc
     6181 catgagcttt aacacaatca ttgactggaa tagctgtact gcggagcaac aacgccagct
     6241 gttaatgcgc ccggcgatct ccgcttctga aagcattacc cgcactgtta acgatattct
     6301 cgataacgtg aaagcacgcg gcgatgaggc cctgcgggaa tacagcgcga agtttgataa
     6361 aaccacggtt accgcactga aggtgtctgc tgaggaaatt gccgccgcta gcgaacgcct
     6421 gagcgacgag ctaaaacagg cgatggcggt ggcagtgaag aatattgaaa ccttccacac
     6481 tgcgcaaaaa ctgccgccgg tagatgtaga aacgcagcca ggcgtgcgtt gccagcaggt
     6541 cacgcgcccg gtcgcttcag ttgggttgta tattcctggc ggctccgccc cgctcttctc
     6601 aacggtatta atgctggcga ctccggcgcg tattgcgggc tgtaaaaaag tggtgctgtg
     6661 ctcaccgccg ccgattgccg atgagatcct ttatgcggcg cagctgtgcg gtgtgcagga
     6721 cgtgtttaac gtcggcggcg cacaggccat tgccgcactg gcgtttggta cggaatctgt
     6781 gccgaaagtg gacaaaatct tcgggccggg caacgccttt gtcaccgaag cgaaacgtca
     6841 ggtgagccag cgtctggacg gtgcggcgat cgatatgccc gcaggcccgt cggaagtgtt
     6901 ggtcattgcc gacagtggcg caacgccgga tttcgtggct tctgatttgc tttctcaggc
     6961 tgaacacggc ccggactcac aggtgatttt actgacgccc gctgctgata tggcgcgtcg
     7021 cgttgccgag gctgtcgaac gccagctggc agaactgccg cgtgctgaaa ccgcccgtca
     7081 ggcactgaac gccagccgcc tgatcgtgac taaagattta gcgcagtgcg tagagatctc
     7141 caaccagtac ggcccggagc acctgatcat ccagacccgc aacgcccgcg atctggtcga
     7201 tggcattacc agtgccggtt cggtatttct tggtgactgg tcactggaat cggcaggtga
     7261 ttacgcctcc ggcaccaacc acgttctgcc gacttacggt tacaccgcca cctgttccag
     7321 cctcgggctg gcagatttcc agaagcgcat gaccgtgcag gaactgtcga aagaaggttt
     7381 ctccgcgctg gcttcgacca ttgaaacatt ggccgccgcc gagcgcctga ccgcccataa
     7441 aaatgccgtt actttacgtg ttaacgccct taaggagcaa gcatgagcat cgtgactatt
     7501 accgatttag cgcgtgaaaa cgtgcgcaac ctgacgccgt atcagtcggc gcgtcgtctg
     7561 ggcggtaacg gcgatgtctg gctgaacgcc aacgaatatc ccacagccgt ggaatttcag
     7621 cttactcagc aaacgctcaa ccgctacccg gaatgtcagc cgaaagcggt gattgaaaat
     7681 tacgcgcaat atgcaggcgt aaaaccggaa caggtgctgg tcagccgtgg cgcggacgaa
     7741 ggtattgaac tactgattcg cgctttttgc gaaccgggta aagacgccat cctctactgc
     7801 ccgccaacgt acggcatgta cggcgtcagc gccgaaacca ttggcgtcga gtgccgcaca
     7861 gtgccgacgc tggacaactg gcaactggat ttacagggca tttccgacaa gctggaaggc
     7921 gtaaaagtgg tctatgtttg cagccccaac aacccgaccg ggcaactgat caatccgcag
     7981 gattttcgca ctctgctgga gttaacccgc ggtaaagcga ttgtggttgc cgatgaagcc
     8041 tatatcgagt tttgcccgca ggcatcgctg gctggctggc tggcggaata tccgcacctg
     8101 gctattttgc gcacactgtc gaaagctttt gctctggcgg gccttcgttg cggatttacg
     8161 ctggcaaacg aagaagtcat caacctgctg atgaaagtga ttgcccccta cccgctctcg
     8221 acgccggttg ccgacattgc ggcccaggcg ttaagcccgc aggggatcgt cgccatgcgt
     8281 gagcgggtgg cgcaaattat tgcagaacgc gaatgcctga ttgccgcact gaaagagatc
     8341 ccctgcgtgg agcaggtttt cgactctgaa accaactaca ttctggcgcg ctttaaagcc
     8401 tccagtgcag tgtttaaatc tttgtgggat cagggcatta tcttacgtga tcagaataaa
     8461 caaccctctt taagcggctg cctgcgaatt accgtcggaa cccgtgaaga aagccagcgc
     8521 gtcattgacg ccttacgtgc ggagcaagtt tgatgagtca gaagtatctt tttatcgatc
     8581 gcgatggaac cctgattagc gaaccgccga gtgattttca ggtggaccgt tttgataaac
     8641 tcgcctttga accgggcgtg atcccggagc tgctgaagct gcaaaaagcg ggctacaagc
     8701 tggtgatgat cactaatcag gatggtctgg gaacacaaag tttcctgcag gcggattttg
     8761 atggcccgca caacctgatg atgcagatct tcacctcgca aggcgtgcag tttgatgaag
     8821 tgctgatttg tccgcacctg cccgccgatg agtgcgactg ccgtaagccg aaagtaaaac
     8881 tggtggaacg ttatctggct gagcaagcga tggatcgcgc taacagttat gtgattggcg
     8941 atcgcgcgac cgacattcaa ctggcggaaa acatgggtat taatggttta cgctacgacc
     9001 gcgaaaccct gaactggccg atgattggcg agcaactcac taaacgagac cgttacgccc
     9061 atgtagtgcg caacaccaaa gagacgcaaa ttgacgtcca ggtgtggctg gatcgcgaag
     9121 gtggcagcaa gattaacacc ggcgttggct tctttgatca catgctggat cagatcgcca
     9181 cccacggcgg tttccgtatg gaaatcaacg tcaaaggcga cctctatatc gacgatcacc
     9241 acaccgtcga agataccggc ctggcgctgg gtgaagcgtt aaaaattgcc cttggcgaca
     9301 aacgcggtat ttgccgcttt ggttttgtgc taccgatgga cgaatgcctt gcccgctgtg
     9361 cactggatat ctctggtcgc ccgcacctgg aatataaagc cgaatttacc taccagcgcg
     9421 tcggcgatct cagcaccgag atgatcgagc acttcttccg ttcgctgtct tacaccatgg
     9481 gcgtgacgct acacctgaaa accaaaggta aaaacgatca ccaccgtgta gagagcctgt
     9541 tcaaagcctt tggtcgcacc ctgcgccagg ccatccgcgt ggaaggcgac accctgccct
     9601 cgtcgaaagg agtgctgtga tgaacgtggt gatcctggat accggctgcg ccaacctgaa
     9661 ctcggtgaag tctgccattg cgcgccacgg ttatgaaccc aaagtcagcc gtgacccgga
     9721 cgtcgtgttg ctggccgata aactattttt acccggcgta ggcaccgcac aagcagcgat
     9781 ggatcaggtg cgtgagcgcg aactgtttga tctcatcaaa gcctgtaccc aaccggtgct
     9841 gggcatctgc ttagggatgc aactgcttgg gcggcgcagc gaagagagca acggcgtcga
     9901 cttgctgggc atcatcgacg aagacgtgcc gaaaatgacc gactttggtc tgcccctgcc
     9961 gcatatgggc tggaaccgcg tttacccgca ggcaggcaac cgcctgtttc aggggattga
    10021 agatggcgcg tacttttact ttgttcacag ctacgcaatg ccagtcaatc cgtggaccat
    10081 cgcccagtgt aattacggcg aaccgttcac cgcggcggta caaaaagata acttctacgg
    10141 cgtgcagttt cacccggagc gttctggtgc cgctggcgct aagttgctga aaaacttcct
    10201 ggagatgtga tgattattcc ggcattagat ttaatcgacg gcaccgtggt gcgtctccat
    10261 cagggcgatt acggcaaaca gcgcgattac ggtaacgacc cgctgccgcg cttacaggat
    10321 tacgcagcgc agggtgccga agtgctgcat ctggtggatc tgaccggagc aaaagatccg
    10381 gcaaaacgtc aaatcccgct gattaaaacc ctggtcgcgg gcgttaacgt tcctgtgcag
    10441 gttggcggcg gcgtgcgtag cgaagaagac gtggcggcgt tactggaagc gggcgttgcg
    10501 cgcgtggtgg ttggctccac cgcggtgaaa tcaccagaaa tggtgaaagg ctggtttgaa
    10561 cgtttcggtg ccgatgcctt agtactggcg ctggatgtcc gtattgacga gcaaggtaac
    10621 aagcaggtgg cggtgagcgg ctggcaagag aactcgggcg tttcactgga acaactggtg
    10681 gaaacctatc tgcccgtcgg cctgaaacat gtgctttgta ccgatatctc gcgcgacggc
    10741 acgctggcag gttctaacgt ctctttatat gaagaagtgt gcgccagata tccgcaggtg
    10801 gcatttcagt cctccggcgg cattggcgac attaacgatg ttgctgctct acgtggtact
    10861 ggcgtgcgcg gcgtaatagt tggtcgtgca ttactggaag gtaaattcac cgtgaaggag
    10921 gccatcgcat gctggcaaaa cgcataattc cgtgtctcga cgttcgtgat ggtcaggtgg
    10981 tgaaaggcgt acagtttcgc aaccatgaaa tcattggcga tatcgtgcca ctggcaaaac
    11041 gctacgccga agaaggtgca gatgaactgg tgttctacga tatcaccgct tccagcgatg
    11101 gccgtgtggt agataaaagc tgggtatctc gcgtggcgga agtgatcgac attccgtttt
    11161 gtgtggcggg tgggattaag tctctggaag atgccgcgaa aattctttcc tttggcgcgg
    11221 ataaaatttc cataaactcg cctgcgctgg cggacccgac gttaattact cgcctggccg
    11281 atcgctttgg cgtgcagtgt attgtagtcg gtattgatac ctggtacgac ggcgaaaccg
    11341 gtaaatatca tgtgaatcaa tataccggcg atgaaagccg cacccgcgtc actcagtggg
    11401 aaacactcga ctgggtacag gaagtgcaaa aacgcggtgc cggagaaatc gtcctcaaca
    11461 tgatgaatca ggacggcgtg cgtaacggtt acgacctcga acaactgaaa aaagtgcgtg
    11521 aagtttgcca cgtcccgcta attgcctccg gtggcgcggg caccatggaa cacttcctcg
    11581 aagccttccg cgatgccgac gttgacggcg cgctggctgc ttccgtattc cacaaacaaa
    11641 taatcaatat tggtgaatta aaagcgtacc tggcaacaca gggcgtggag atcaggatat
    11701 gttaacagaa caacaacgtc gcgaactgga ctgggaaaaa accgacggac ttatgccggt
    11761 gattgtgcag cacgcggtat ccggtgaagt gttaatgctg ggctatatga acccggaagc
    11821 cttagacaaa accatcgaaa gcggcaaagt caccttcttc tcgcgcacta aacagcgact
    11881 gtggaccaaa ggcgaaacgt cgggcaattt cctcaacgta gtaaatattg cgccggactg
    11941 cgacaacgac acgctactgg tactggcgaa tcctatcgga ccaacctgcc acaaaggcac
    12001 cagcagctgc ttcggcgaca ccgctcacca atggctgttc ctgtatcaac tggaacaact
    12061 gctcgccgaa cgtaaatctg ccgacccgga aacctcttac accgccaaac tgtatgccag
    12121 cggcaccaaa cgcattgcgc agaaagtggg cgaagaaggc gtggaaaccg cactggccgc
    12181 gacggtacat gaccgctttg agctcaccaa cgaggcgtct gatttgatgt atcacctgct
    12241 ggtattgttg caggatcagg atctggattt aacaacggtg attgagaacc tgcgtaaacg
    12301 tcatcagtga gttgcggggt aagcggatgc gatgttgtcg tcgcatccgg caaaaaaaac
    12361 gggcaaggtc tcaccaccct gcccattttc tttaaaaccg aaaagattac ttcgcgttgt
    12421 aattacgcag agcgttacgc cccagcacaa tccccgcgcc aaccatgccg cccagcagca
    12481 ctgccaaaat caaggtaatt gcctttttcg ggctatcgcg acgaataggt aacgtcggtt
    12541 tcattacata gcggtaagca tgaatatcaa gatcatcaac ctttaagctt tcgatatcca
    12601 gcaggttttg acgtgtctga tagtagtttg atgagaacac caacggacgg gtcgcctcat
    12661 gcttaatcat cgactccagc gcttcgctcc ccaaaaggaa caacgtatct tgtgtcacat
    12721 cttcagtctg ctgaacctgt ggctttgtca cctgcgcctg attcgcatac tgcaacgctt
    12781 cctgaatctg acggatacgc agatctttct gctcctgcgc gactacttcc tgagttttca
    12841 gagaatcctg caagtttttc atacgcagcg cgatgttgtc tttcaggtct ttttccagtt
    12901 cttcgtttac ctggtcgtca acttgctgga tatactgcgc cagctgtttc tgcgcacctt
    12961 cagctgtttc accaacataa gaaaccgcta aaggtaagga ctgattttta acggtaggct
    13021 caatggtcag tttttcaggt tcttcctgat tatccagcgt ttccgctaat gctgagaaag
    13081 cagtgctgta acgtccgata agtgaagtct gaatgtcgga gactttcggt acagccggac
    13141 cgtaaatcac attcatcgcg ttggtataac cagcgatttg ccccacatca ggttgagtaa
    13201 cgatagctgt ggaagtccac ttttctttgg caacggccag ataccctata gccagggcaa
    13261 tagccacaat gacagagatg ataattgtca tcttgccgcg ccacaactgc accaggaaat
    13321 caatcaaatc aatctgttcc gggtcatggt tttgcccaga aacattatta ttttctactc
    13381 tcatcattac cctaacggac gaaataattt taagtcgcca tagtttagcg ataatcgcca
    13441 tgaatgctat aggataaatg ataaaaataa tgaattatta caaagggaac ataagagaat
    13501 cagggcgaaa atcgcactga taacaagatg ttagtcgctg ccaaaaagat cgcgggtgta
    13561 gactttgtct gccacatcct taagctcttc tgccatacgg ttggagataa tgacgtcggc
    13621 ttgtatagtc agcttagaac agctttgatt gtaaaatgag cacgttacca gaaaaaacag
    13681 ccaggtttag aactgatacc gattatcttt ttttcttgtc taacggtatg atttaacttt
    13741 cagttatgcc agatgaaaat tggctatatt ctagcctgag cgaggattat aaatgaaatt
    13801 tctggttacg ggagctgctg gctttatcgg tttctatgta agtaaacggc ttcttgcagc
    13861 tggtcatcag gttgtaggga ttgacaactt aaatgattat tacgatgtca acctcaaaca
    13921 agcacgactt gatttactca agcacgacaa tttcagtttt tataaaattg acctggccga
    13981 tcgcgagaaa atggccgcac tgtttgcaga cgagcggttc gatcgcgtaa taaacctcgc
    14041 tgcgcaagcc ggtgtacgtt actcccttga aaatcccaat gcatatgcag atgcaaacct
    14101 gattggattc ctgaacatac tggaaggatg ccgccataat aatgttcagc atctacttta
    14161 cgcttcctcc agttctgttt atggcatgaa ccgcaagatg cctttctcta cagatgactc
    14221 tgtagatcat cctgtttcgc tttatgcagc aactaaaaaa gcgaatgaac tcatggccca
    14281 tacgtattct catttgtatg gcttaccgac cacagggctg cgtttcttta cggtttatgg
    14341 tccatgggga cgtccggata tggcattatt taaatttact aaagccatgc tggaaggtaa
    14401 aagcattgat gtttacaact tcggcaaaat gaagcgtgac tttacttata ttgatgatat
    14461 tgccgaagct attattcgct tacaggatgt tattccagaa aaagatccac agtggactgt
    14521 agaaactgga tcgcctgcaa caagttcagc accgtatcgt gtctataaca ttggtaatag
    14581 ttcgcctgtg gagttgatgg actatatcaa tgccttagaa gaggctctgg gtattgaagc
    14641 caacaagaat atgatgcctc tccaacccgg tgatgtactg gaaaccagtg ctgatacaaa
    14701 agcactgtat gacgtaatag gattcaaacc tgaaacgtca gttaaagagg gggtaaagaa
    14761 ctttgtagaa tggtatcgta acttctataa agtttgattt tacaaaacca taagaaaagg
    14821 ccctaatata ttagggcctt ttcttagaat aaaacaaaat aattaatcat tgccaaacaa
    14881 gtcgcgcgta taaactttat ctgctacatc agccagatcg gcagacatac ggttagaaat
    14941 aataacatca gcttcttgtt tgaacgcatc cagatcacgt accacgcgcg aacggaaaaa
    15001 atcgtcctct ttcatcgctg gttcataaac gattactggc acacctttcg ccttgattcg
    15061 cttcataata ccctgaatcg aggaagcacg gaaattgtct gaaccactct tcataatcag
    15121 acgataaacg ccaacaactt tcggtttacg tgcaaggata gaatcggcaa taaagtcttt
    15181 tcgcgtgcgg ttagcatcaa caattgccga aatcagatta ttcggtacag attgatagtt
    15241 tgccagtaac tgcttggtat ctttcggcag acaataacca ccataaccaa atgagggatt
    15301 gttgtagtga ttaccgatac gcggatcaag gcaaaccccc tcaataattt ggcgggagtt
    15361 aagacccaga ctttcagcgt aactatctag ttcattaaaa taagccacac gcatcgccag
    15421 ataagtattg gcaaaaagtt taatcgcctc agcctcggtt gagtcagtaa acaatattgg
    15481 tatgtcttgc ttaatagcgc cttcctgtaa taacgctgca aaacgttcag cgcgatcgga
    15541 ttgctcacca atcacaatgc gtgatggatg caagttatca taaagagctt tcccttcgcg
    15601 taaaaactcc ggcgaaaaga tcacattttc aataccaaac cgttctttaa tggactctgt
    15661 aaaaccaaca gggatagttg attttataat cattaccgcg ttgggattaa tttctgtcac
    15721 atcgcgaatg accgcttcca cgcttgaggt attaaaataa tttgttttcg ggtcataatc
    15781 ggtaggcgtg gcaataataa cataatcggc atttttatac gcgtcatact tatctgtcgt
    15841 agcgcggaaa ttgagatctt tagtcgccag atactcttca atctccttat caacaatcgg
    15901 tgactgcttc ttgttaagca tgtctacttt ggcctgaacg atatccagtg caaccacttc
    15961 gtgattttgc gcaatcagaa taccatttga aagaccaaca taacctgttc ctgaaattgt
    16021 tattttcatt agctctgact tcttccggtt aaacatttag agtggtcatt aatccaccac
    16081 cacaacttca tctactgcgg gatttttaac gctaatgtag atgaactgtc aaggcagcga
    16141 tcctgctgtg cggcgctgta ttatatcgcg tttttaacta taaattataa aaaaaggccc
    16201 taacctgccg ctttgtataa taaaaaagcc cggagggttt ctccgggcct tgcattgatt
    16261 aattgattta aatcagatta atccagccat tcggtatgga acacaccgtc tttatcaatg
    16321 cgtttataag tatgcgcacc gaaatagtca cgctgtgcct ggatcaggtt cgcaggcaga
    16381 acagcggcac ggtagctatc ataataggca acagcagcgg cgaaggtcgg aaccgggata
    16441 ccgttctgta ctgcgtaagc gacgacatcg cgcagcgcct gctggtagtc atcggcgatt
    16501 tgcttgaagt acggggccag cagcaggtta gcgatctgcg gattttcggc atatgcatcg
    16561 gtgattttct gcaggaactg cgcacggatg atgcagccag cacggaaaat cttcgcgatt
    16621 tcgccgtagt tcagatccca gttgtactct tcagacgccg cacgcagttg agagaacccc
    16681 tgagcgtaag aaacgatttt gcccagatac agtgcacggc gaactttttc gatgaactca
    16741 gccttgttgc cagctggctg cgcttgtggg ccagagagaa ctttagacgc agcaacgcgc
    16801 tgatctttca gagaagagat gtatcgtgca aacacagact cggtaatcag cgacagcggt
    16861 tcgccgagat ccagtgcgct ctggctggtc catttgcccg tacctttgtt tgccgcttca
    16921 tccaggatca catcaaccag gtagttaccg tcttcatctt ttttagtgaa gatgtctttg
    16981 gtgatatcga tcagatagct gctcagttca ccgttattcc actcggtaaa ggtctgcgcc
    17041 aactcttcgt tggaaaggtt caggccgcct ttaagtagag aataggcttc agcaatcagt
    17101 tgcatatcac cgtattcaat accgttgtga accatcttca catagtgacc tgcaccatcg
    17161 gcaccaatat aggtaacgca tggctcaccg tcttcagcca ctgcggcgat tttggtcagg
    17221 atcggtgcaa ccagttcata ggcttctttc tgtccgccag gcataatgga aggacctttc
    17281 agcgcacctt cttcaccgcc ggaaacaccg gtaccaatga agttaaagcc ttctgcagaa
    17341 agctcacgat tacgacgaat ggtgtcctgg aagaaggtat taccaccatc aatgatgatg
    17401 tcacctttat cgaggtatgg cttgagggaa tcaatagcag catccgtgcc tgcacctgct
    17461 ttcaccatta acaggatgcg acgaggcgtt tccagagatt caacaaactc tttcaccgta
    17521 tagtaaggaa ccagtttctt gcctggattt tcggcaatca cttcttccgt cttctcacgg
    17581 gaacggttga aaatagagac ggtataacca cggctttcga tgttgagcgc aaggttgcgc
    17641 cccatcactg ccataccgac gacgccgatc tgttgctttg acattgttta ctcctgtta
//
LOCUS       DAZPEB010000069        17608 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZPEB010000069 DAZPEB010000000
VERSION     DAZPEB010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17608
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.070"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            444..725
                     /gene="drpB"
                     /locus_tag="ACHURJ_002058"
     CDS             444..725
                     /gene="drpB"
                     /locus_tag="ACHURJ_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFR1281005.1"
                     /translation="MEERLSRSPGGKLALWAFYTWCGYFVWAMARYIWVMSRIPDAPV
                     SGFESDLGSTAGKWLGALVGFLFMALVGALLGSIAWYTRPRPARSRRYE"
     gene            765..1460
                     /locus_tag="ACHURJ_002059"
     CDS             765..1460
                     /locus_tag="ACHURJ_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HFR1281006.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCH
                     VIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTARGKQLAQHNAHFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            1527..2945
                     /gene="dcm"
                     /locus_tag="ACHURJ_002060"
     CDS             1527..2945
                     /gene="dcm"
                     /locus_tag="ACHURJ_002060"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFR1281007.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMAKGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            2926..3396
                     /gene="vsr"
                     /locus_tag="ACHURJ_002061"
     CDS             2926..3396
                     /gene="vsr"
                     /locus_tag="ACHURJ_002061"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFR1281008.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGSPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(3385..4305)
                     /gene="yedA"
                     /locus_tag="ACHURJ_002062"
     CDS             complement(3385..4305)
                     /gene="yedA"
                     /locus_tag="ACHURJ_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFR1281009.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLMAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            4478..5395
                     /locus_tag="ACHURJ_002063"
     CDS             4478..5395
                     /locus_tag="ACHURJ_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFR1281010.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDSLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKIRGQAH"
     gene            5474..5656
                     /locus_tag="ACHURJ_002064"
     CDS             5474..5656
                     /locus_tag="ACHURJ_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFR1281011.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            5827..7521
                     /gene="dgcQ"
                     /locus_tag="ACHURJ_002065"
                     /pseudo
     CDS             5827..7521
                     /gene="dgcQ"
                     /locus_tag="ACHURJ_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
     gene            complement(7518..8333)
                     /locus_tag="ACHURJ_002066"
     CDS             complement(7518..8333)
                     /locus_tag="ACHURJ_002066"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFR1281012.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGVPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(8631..8847)
                     /gene="yodD"
                     /locus_tag="ACHURJ_002067"
                     /pseudo
     CDS             complement(8631..8847)
                     /gene="yodD"
                     /locus_tag="ACHURJ_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
     gene            9010..9198
                     /gene="dsrB"
                     /locus_tag="ACHURJ_002068"
     CDS             9010..9198
                     /gene="dsrB"
                     /locus_tag="ACHURJ_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFR1281013.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            complement(9242..9865)
                     /gene="rcsA"
                     /locus_tag="ACHURJ_002069"
     CDS             complement(9242..9865)
                     /gene="rcsA"
                     /locus_tag="ACHURJ_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFR1281014.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(10150..10935)
                     /gene="fliR"
                     /locus_tag="ACHURJ_002070"
     CDS             complement(10150..10935)
                     /gene="fliR"
                     /locus_tag="ACHURJ_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFR1281015.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFVIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            complement(10944..11213)
                     /gene="fliQ"
                     /locus_tag="ACHURJ_002071"
     CDS             complement(10944..11213)
                     /gene="fliQ"
                     /locus_tag="ACHURJ_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFR1281016.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            complement(11291..11959)
                     /gene="fliP"
                     /locus_tag="ACHURJ_002072"
     CDS             complement(11291..11959)
                     /gene="fliP"
                     /locus_tag="ACHURJ_002072"
                     /inference="COORDINATES: protein motif:HMM:NF013011.4"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFR1281017.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILRPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPPPLL
                     CPLN"
     gene            complement(11959..12324)
                     /gene="fliO"
                     /locus_tag="ACHURJ_002073"
     CDS             complement(11959..12324)
                     /gene="fliO"
                     /locus_tag="ACHURJ_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFR1281018.1"
                     /translation="MNNHATVQSSTPVSAAPLLQVSGALIAIIALILAAAWQVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            complement(12327..12740)
                     /gene="fliN"
                     /locus_tag="ACHURJ_002074"
     CDS             complement(12327..12740)
                     /gene="fliN"
                     /locus_tag="ACHURJ_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFR1281019.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMHRLSR"
     gene            complement(12737..13741)
                     /gene="fliM"
                     /locus_tag="ACHURJ_002075"
     CDS             complement(12737..13741)
                     /gene="fliM"
                     /locus_tag="ACHURJ_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFR1281020.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            complement(13746..14210)
                     /gene="fliL"
                     /locus_tag="ACHURJ_002076"
     CDS             complement(13746..14210)
                     /gene="fliL"
                     /locus_tag="ACHURJ_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFR1281021.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALNTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            complement(14315..15442)
                     /locus_tag="ACHURJ_002077"
     CDS             complement(14315..15442)
                     /locus_tag="ACHURJ_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFR1281022.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLISDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADQNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            complement(15439..15882)
                     /gene="fliJ"
                     /locus_tag="ACHURJ_002078"
     CDS             complement(15439..15882)
                     /gene="fliJ"
                     /locus_tag="ACHURJ_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFR1281023.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERLSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            complement(15901..17269)
                     /gene="fliI"
                     /locus_tag="ACHURJ_002079"
                     /pseudo
     CDS             complement(15901..17269)
                     /gene="fliI"
                     /locus_tag="ACHURJ_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
     gene            complement(17269..>17608)
                     /locus_tag="ACHURJ_002080"
     CDS             complement(17269..>17608)
                     /locus_tag="ACHURJ_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFR1281024.1"
                     /translation="ASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
BASE COUNT         4291 a         4429 c         4676 g         4212 t
ORIGIN      
        1 ataaatgtat atcaatatgt tatgagttga gcggaaacat agggggtatc aatgattgtt
       61 gcgccaaacg gctggtgtga tcaggcgcac attaatgaag gcataaaaaa aagccggcat
      121 catgccggcc caaaaaatat aaacaagacg ataaaaggct aataacggaa gcatcatgac
      181 acagaataat aaaaaggtat aattgtaatc agtgctaata tattgattaa atgaattaat
      241 aaagccgata tcaaagataa ataaaatcgc tcaaaacgat ttttcctgta ttcactgccg
      301 ttgcgcaaaa tttatctatt tgttcaaaaa atgattgagt cttgactggc tcatccaatg
      361 tggaaaaatg tgacttttat cacataatag tactaagtct gaattttccg ggttatctca
      421 aaatggaata cggttcgaca aagatggaag agagactctc tcgcagccct ggcggcaaac
      481 tggctttgtg ggctttctat acatggtgtg gctatttcgt ctgggcaatg gcgcgttaca
      541 tctgggtgat gagccggatc cccgatgccc ccgtgtcggg atttgaaagc gatttaggct
      601 ctaccgcggg aaagtggtta ggggcactgg tcggattttt atttatggct ctggtcgggg
      661 cattgttagg cagcattgcc tggtacaccc gacctcgtcc cgcacgtagc agacgctatg
      721 aataaaaact gctaaggtgg cgactctttg gcaacggagc gcgtatggac ttacaacact
      781 ggcaggcaca gttcgaaaac tggttgaaaa accatcatca gcaccaggac gcggcccatg
      841 acgtgtgtca ttttcgccgc gtctgggcta cggcacagaa gctggcggca gatgacgatg
      901 tcgatatgct ggtgatttta accgcctgtt attttcacga tattgtgagc ctggcgaaaa
      961 atcatccgca aaggcagcgt tcttcaatcc tggcggcaga agagacgcgt cgcctgctcc
     1021 gtgaagagtt cgagcaattt ccggcggaga aaatcgaggc cgtttgtcat gtcattgccg
     1081 ctcacagttt cagcgcgcaa atcgccccct taacaacgga agctaaaatc gtccaggatg
     1141 cggatcggct ggaagccctg ggggcgattg gcctggcgcg cgtgtttgcc gtttcagggg
     1201 cattgggcgt ggcgctgttt gatggtgaag atccgttcgc acagcatcgc ccgcttgatg
     1261 ataaacgcta cgcgctggat catttccaga ctaagttgct gaagctaccg caaaccatgc
     1321 aaaccgcaag aggcaagcag ctggcgcagc acaacgcgca ttttttagtc gagtttatgg
     1381 cgaagctcag tgccgaactg gcgggggaga atgaaggtgt cgatcacaag gtgatagatg
     1441 cgttttcacc cgcgggataa gcgcgtggcc ctaaatggct gtaattatgt taacctgtcg
     1501 gccatctcag atggccggtt aaatctatgc aggaaaatat atcagtaacc gattcataca
     1561 gcaccgggaa tgccgcacag gcaatgctgg agaaactgct gcaaatttat gatgttaaaa
     1621 cgctggtggc gcagcttaat ggtgtgggtg agaatcactg gagcgcggca attttaaaac
     1681 gtgcgctggc gaatgactcg gcatggcacc gtttaagtga gaaagagttc gcccatctgc
     1741 aaacgttgtt acccaaacca ccggcacatc atccgcatta tgcgtttcgc tttatcgatc
     1801 tatttgccgg aattggcggc atccgtcgcg gttttgaatc gattggcgga caatgcgtgt
     1861 ttaccagcga atggaacaaa catgcggtac gcacttataa agccaaccat tattgcgatc
     1921 cggcgacgca tcattttaat gaagatatcc gcgatatcac cctcagccat aaagaaggcg
     1981 tgagtgatga ggcggcggcg gaacatattc gtcaacacat tcctgaacac gatgttttac
     2041 tggccggttt cccttgtcag ccattttcgc tggctggcgt atcgaaaaag aactcgctcg
     2101 ggcgggcgca cggttttgcc tgcgataccc agggcacgct gttttttgat gtggtacgca
     2161 ttatcgacgc gcgtcgtccg gcgatgtttg tgctcgaaaa cgtcaaaaac ctgaaaagtc
     2221 acgaccaggg taaaacgttc cgcatcatca tgcagacgct ggacgaactg ggctatgacg
     2281 tggctgatgc agaagataac gggccggacg atccgaaaat catcgatggt aaacattttc
     2341 tgccgcagca ccgtgaacgc atcgtgctgg tgggttttcg tcgcgatctt aatctgaaag
     2401 ccgattttac tctgcgtgat atcagcgaat gtttccctgc acagcgagtg acgctggcgc
     2461 agctgttgga cccgatggtc gaggcgaaat atatcctgac gccggtgctg tggaagtacc
     2521 tctatcgata tgcgaaaaaa catcaggcgc gcggtaacgg cttcggttat ggaatggttt
     2581 atccgaacaa tccgcaaagc gtcacgcgta cgctgtctgc gcgttattac aaagatggcg
     2641 cggaaatttt aatcgatcgc ggctgggata tggccaaagg tgagaaagac tttgacgatc
     2701 cgctgaatca gcaacatcgt ccacgtcggt taacgcctcg ggaatgcgcg cgcttaatgg
     2761 gttttgaagc gccgggagaa gcgaaattcc gcattccggt ttcggacact caagcctatc
     2821 gccagttcgg taactcggtg gtcgtgccgg tctttgccgc ggtggcaaaa ctgcttgagc
     2881 caaaaatcaa acaggcggtg gcgttgcgtc agcaagaggc acaacatggc cgacgttcac
     2941 gataaggcca ctcgcagcaa aaatatgcgc gcgattgcca cgcgtgatac ggcgattgag
     3001 aagcgcctcg ccagtctgtt aaccgggcag ggcctggcgt ttcgcgttca ggacgccagt
     3061 ctgcccggaa gcccggattt tgtcgttgat gaatatcgct gcgtgatatt tacccatggc
     3121 tgcttctggc atcatcatca ctgctatctg tttaaagtgc ctgcgactcg aaccgagttc
     3181 tggctggaga agataggtaa aaatgttgaa cgcgatcgcc gcgatatcag tcgtttgcag
     3241 gaactcggct ggcgcgtact gattgtctgg gagtgtgcat tacgtgggcg cgagaagctg
     3301 acggatgaag cgcttaccga gcgtctggaa gagtggatct gcggcgaagg tgccagcgcg
     3361 cagatcgaca cgcaggggat tcatttactc gcttgatgcg tcctgaataa ctggcgcaac
     3421 tacgggtttt gccgggaaga gatattttcc caacgtgacc agtaccaccg cgaagacaat
     3481 tacgccgagc gccagccatt caatcttcga cagcgtctct ccgcccagtc ccgtacccag
     3541 caagaccgcg accaccgggt taacgtaggc gtagctggtg gcgagagccg gactgacatt
     3601 acggattaaa tacatataag cgttgatggc gataatcgaa ccaaacagcg ccagatagcc
     3661 gaccgcaagg aagcctgaaa gggaagggag cgccgtcagt ttttcacctg caatcatcga
     3721 cgcgatcatt aacaccacgc ctgccgccag catctcaatc gcacccgcca tcatccctac
     3781 aggtaaggta atgcgcgagc cataaaccga gccaaacgcc cagctaatcg agccgattaa
     3841 aatcagaatc gcgccccacg gattgccgct taaatttcca ccgctattga gcatgatgat
     3901 tccggcaagc ccaatggcga tacccaccca ttccaatttg cgcgttttaa tgccaaacag
     3961 gcggctgaag cacagggtaa agaggggcac ggttgcaacc acaacggcgg cgatgccgga
     4021 aggaacattt tgatgctcgg caaccgtcac catgccatta ccgacagcca gcaataacag
     4081 gccaatcagc gcggcattga gcagcggacg tagcgggggg agcttgtgtc cgcgcagtag
     4141 caaaaatgcc atcaataaaa taccggctgc caggaatcga acgcctgcca tcattaacgg
     4201 aggccagctt tccacgccaa tccgaatgac aaaataggtt gagccccaaa tgatatacaa
     4261 cgcaaacagc gcgccaaaaa gcggtaacaa ctggcggaaa cgcataatcc ctcacggtgg
     4321 aaataaaaag gtggttcata gtaaacgtga aaatcattct gctggcgaga gatataattg
     4381 catttgattg ttaaaaaaat gttgacctgt gaagcaagta tcagagctgc cgtttttgct
     4441 tcatacttac acccttcaac aataaaaatg agagggaatg cttttggccg ggagtagttt
     4501 actgacgttg ctcgatgata tcgccacact actggacgat atctccgtga tgggcaaact
     4561 ggcggcgaag aaaaccgccg gtgtattagg ggatgactta tcgctcaatg cgcaacaagt
     4621 ttcaggcgtg cgggccaacc gggaacttcc cgtggtctgg ggcgtggcga aaggatcgct
     4681 gattaataaa gtgattctgg tgccgctggc gctgatcatc agtgcgttta tcccgtgggc
     4741 gattacgcct ctgttgatga ttggtggcgc gtttctctgc tttgaaggag tagagaaagt
     4801 gctgcatatg ctggaggcgc gtaaacataa agaagatccg gcgcagagcc agcagcgtct
     4861 ggagaagctg gcggcgcagg attcgctgaa gtttgaaaag gacaaaataa aaggggcgat
     4921 tcgtaccgat tttatattgt ctgccgaaat cgtcgccatc acgctgggga ttgtggccga
     4981 agcgccgttg cttaatcagg tgctggtgct ttcaggcatc gcgctggtag tgaccgtggg
     5041 cgtctacggt ctggtagggg ttatcgttaa gattgatgac ctggggtatt ggctggcgga
     5101 aaaatccagc gcgctgatgc aggcattagg taaagggtta ttgatcattg cgccctggct
     5161 gatgaaagcg ttatcgattg tcggcacgct ggcgatgttc ctcgtcggcg gcgggattgt
     5221 ggtacatggt attgcgccgc tgcatcacgc cattgaacat ttcgctgggc agcaaagtgc
     5281 agtggtggcg atgatattac cgactgtttt aaatctgatt cttggattta tcatcggcgg
     5341 catcgtggtg ctgggagtga aagccgtagc gaaaatacgc ggtcaggcac attaatactc
     5401 gcacaggcta ttatgcaaaa ttcgccatct tcaactaagg tgaacaagtt tcactcgaaa
     5461 aaaggaggcg agtatgagct ttatggttag tgaggaagtt acggttaaag agggcggccc
     5521 gcggatgatt gtcaccggat actccagcgg tatggttgag tgtcgttggt atgacggtta
     5581 cggggtcaag cgggaagctt ttcatgaaac cgagcttgtt ccgggggagg ggagtcgttc
     5641 tgcggaagaa gtttgaaagg gtatcattaa caccaggccg gatcacttcc ggcctggtgt
     5701 gccaatgcct gcaatgaatg atagtcgcgt cttatcatgc caccattata aaattatcgc
     5761 tgttttgcgg tacgctatcg ccaggttatg ccagaatcat aaaaaagcag gttgggagtc
     5821 gtcagggtgc agcacgagac aaaaatggaa aaccagagct ggttgaaaaa aatcgcacgc
     5881 cgcctggggc ctggtcatgt cgttaatctc tgctttatcg tggtattgct tttttccacc
     5941 ttgctgacct ggcgtgaagt ggtagtgctg gaagatgcct atatctccag tcagcgtaat
     6001 catctggaaa acgtagccaa cgcgctcgat aagcatttgc agtataacgt cgacaaactg
     6061 atctttttgc gtaatggcat gcgcgaagct ctcgtagcgc cactggattt cacttcactg
     6121 cgtaatgctg taaccgagtt cgaacagcat cgcgacgagc acgcctggca aattgaactc
     6181 aaccgacgac gcaccctgtc agtcaatggc gtatcggatg cattagtcag cgaggggaat
     6241 ctcctgtctc gcgaaaatga aagcctcgac aatgaaatta ccgctgcact ggaagttggt
     6301 tacttgctgc gactggcgca caacacctcg tcgatggttg aacaggcgat gtatgtctcg
     6361 cgtgccggat tttacgtttc gacgcagccg accttgttta cgcgcaatgt accaacgcgt
     6421 tattacggct atgtcaccca accctggttt atcggccatt cgcaatgaga aaatcgtcac
     6481 cgcgcggtac gctggtttac ttcgcaaccg gaacacgcca gcaatactga accgcaggtt
     6541 accgtcagtg ttccggtaga cagtaataac tactggtatg gcgtgctggg gatgagtatt
     6601 cccgtgcgta ccatgcagca atttttaaga aacgccatcg ataaaaacct cgatggtgag
     6661 tatcagctct atgacagtaa gctgagattt ttgacctctt ccaatcctga ccatccaaca
     6721 gggaatattt ttgatcctcg tgaactggcc ttgctggcgc aggcgatgga acatgacacg
     6781 cggggcggca ttcgtatgga cagtcgctat gttagctggg aacgtctgga ccatttcgac
     6841 ggtgtgctgg tgcgcgtcca tacgctaagc gaaggcgtgc gcggcgattt cggcagtatc
     6901 agcattgcat taaccctgct gtgggcgctc tttaccacca tgttactcat ctcctggtat
     6961 gtgattcgcc ggatggttag caacatgtat gttctgcaaa gctcgttgca gtggcaggcg
     7021 tggcacgaca ccttaacccg tttatataat cgtggcgcac tgtttgaaaa agctcgtccg
     7081 ctcgcgaaat tgtgtcagac gcaccaacat cctttttctg tcattcaggt cgaccttgac
     7141 cattttaaag cgattaatga ccgctttagt catcaggcgg gcgaccgtgt tctttctcat
     7201 gctgccggat taattagcag ttccttgcgt gcgcaggacg ttgccgggcg ggtcggtggt
     7261 gaggagtttt gtgtgattct gccaggcgcg agtctgacgg aggctgcgga agtcgcagaa
     7321 cgtattcgcc tgaagttaaa tgaaaaagag atgttgattg ctaagagtac gacgatacgc
     7381 atcagtgcct cgttgggggt aagtagcagc gaggaagccg gtgattatga ttttgaacaa
     7441 ctccagtcac tggctgaccg tcggctttat ctcgctaaac aggccgggcg taatcgggta
     7501 tgcgcgagcg ataacgctta acgagcggag aaaaaatggt ccagcccttc acgccatcct
     7561 tccggtcctt cacgctgcgt tcgccagacg cgggccggat cctcatcatg cagatgcacc
     7621 ccttcacggt ttagcccttt cacaatcacc gcgtaatcca ttacctccag taagggcgca
     7681 tcgtttggcc catcgcccag gccaagtgtg gttgggcgtt tgcctgataa ttgttgatag
     7741 gtcgcgataa tccagttggc ggcctgatct tttccggcag aggcatccag gacgtgccag
     7801 aagcgcgcac cctgcataaa ctgtaagccc agttcgttca gacgagcggt aaattgtgcc
     7861 atacgctcgt cactgtcgcg ccagattagc gttaccgacg cctcatgcag ctgcgtcagc
     7921 gccgcctggc tacggcttaa tcccgtccat tcggcgatgg ttgcatcgtc gacatcatcg
     7981 aaagtcgtga atttaaaatg ttctttctcg cgtagcgtat ttaaaacctg gctgatttcg
     8041 ccatggctaa tacctgagat gatgcgtgga aaaccgtcta tatcctgcca ttgttcagca
     8101 agctggatca ctgcgccatt ttcggcaatc agtggtaccc cttgtaaccc caacgttttt
     8161 tgcaagtaca gcatttccgc tgaagtttta ctgctacaga gaatgacggg aacattcgct
     8221 tcgcgtaaac ggctgagcca gggggcagcc ggttgccagt cataactatg actgtccagc
     8281 agggtgccat caagatcgct aaaaaccagt agtggttgtt gaattgaaaa catggtcgcg
     8341 cagttctccc cttaccagga tgattctcaa aacagcacaa ataatcaaag atgatttttt
     8401 cgggagcgat cccgttgacg ccagagccac caaggggtga cgcacgtcac atttctattc
     8461 ataagtagcg ttaatcattc atatcgcgaa tattttcttg tcagcgaaaa aaattgcgga
     8521 taaggtgatg aacacatcag atttcctggt gtaacgaatt ttttaagtgc ttcttgctta
     8581 agcaagtttc atcccgaccc cctcagggtc gggatttttt tattgtgcat tcaacgattc
     8641 acttcagaga cgctgaagtc atgaatatcc agttcgaagg catccgccaa ttcacgccat
     8701 gtatgatatt cacgtccatc gacgctaacg tgttcagaat cgttctggct gcgatgaact
     8761 tatttcattg atcgccgcca gcagggcatc gacatcgacg ctgacctcac gttttgcggt
     8821 atcgctgtac tcttttgcgg ttttcatcat ctttcctcgc aaccgttact ctgttgttgc
     8881 cgccagtcgt taagtccgcc tggcatctct taagtataga ccgtcgagaa aatcagcact
     8941 cgcgcgcgcc tggcgctgcc agaaaaacga aattgttcta cactggcaca aagccacagg
     9001 aggaaaacga tgaaggtgaa tgatcgggta acagtcaaaa cggatggcgg tccgcgtcgt
     9061 cctggcgtgg tactagcagt tgaggagttt agtgaaggca caatgtacct ggtttcgctg
     9121 gaagactacc cgctcggcat ctggttcttt aatgaagcag ggcatcagga cggtatcttt
     9181 gtggagaaag cagagtaatc ttattcagcc tgactggtgg gaaaccacca gtcagaatgt
     9241 gttagcgcat gttgacaaaa ataccattag tcacattatc cgtcagtcgg acgacatggt
     9301 agataacctg tttattatgc gttttgatct tacgtttaat attaccttta tgcgatgaaa
     9361 cggtcttggc tttgatattc atttggtcag agatttgaat ggttccctga cctgccatcc
     9421 acattcgcaa catactcgat tcggttcggc tcaatgataa cgtcggcata tttaaaaaag
     9481 aggttatcgt tgtctctttt ttcagaatat cgccaaggat atcgtcgaga gattccggtt
     9541 taatcgattt agaacttatc aataaatttt ttctgaccaa tagatattca tcaaaatgaa
     9601 cattggcaat tgccataaaa acgataaata acgtattggg atgttgatta atgatgtgct
     9661 tgatgtgctg actgttagaa gcatcatgga tgaaacagtc ctcattaata aacaccactg
     9721 aagggcgctg tgaatcacaa gctatggcaa ggtcatcaac ggtttcaatg tcgttgattt
     9781 ctcttttttt aacccctcta ctcaacagat acccggttaa acctagtcgg gtgtaactac
     9841 ataaatccat aataatcgtt gacatggcat accctcactc aatgcgtaac gataattccc
     9901 cttacctgaa tatttcatca tgactaaacg gaacaacatg ggtcacctaa tgcgccactc
     9961 tcgcgatttt tcaggcggac ttactatccc gtaaagtgtt gtataatttg cctggaattg
    10021 tcttaaagta aatgttgcga tatgtgagtg agcttaaaac aaatatttcg ccgcaggagt
    10081 atcctggaag atgttcgtag gtattatttt tcggataatc cttaggataa catgataaac
    10141 gttacggaat tatattaatg gcaattcact aataatatca gccagcaaat taaacatctc
    10201 actgaataaa tgttcgcaaa aaggtgcaat taacggcatt aatgccgcca ttaaagagat
    10261 accgacagtc agagttaatg gaaatccaat aacaaaaatg gataattgcg gggccatacg
    10321 attcagtaaa cctaatgcca gattcagggt cagcagcaga gtaatgagcg gtaacgccag
    10381 catcagcccg ttaaggaaaa tcaaactccc cgctttggtg agtgccagaa acgcattgct
    10441 gttcaacggt tcgccaccaa ttggcagggt gtgaaaggta tcgaccagca gtgaaatcaa
    10501 ccataaatga ccgttaaatg tcaggaacag cagtaacgcc agcatatcca tgatacgcgc
    10561 taaaacgggc atattgagat ggctggccgg atcgacaaac gtcgcaaatg acagccccat
    10621 ttgcagaccg ataatttcgc cagcggttcg cacagcggca aaggcaaatt gcatggtaaa
    10681 accaagcgca atgccgatca ggatctgctg cacggccagc cacagagcaa agaacgaaaa
    10741 aacaggaaca tcgttggcag gtaatgatgg ggcaatgacg aacgtgatca tcattgccag
    10801 ccccagtttt acccgtttcg gcacgctgcg ttcgctcaga attggtgcgg tagaaatgag
    10861 cgccagcacg cgcagtaacg gccagaagta caggcttaac caggaaagcc attgatcgct
    10921 tgtcacctgc atcattgtac ggcctacccg atgatatacg gcaggttagt gaacaaggtg
    10981 cggacgtaat ccagcaacag attgagcatc cacggtccgg caataataat ggcaataaat
    11041 acggcgatga tttttggaat aaacgacagc gtcatttcgt tgatctgcgt ggcggcctgc
    11101 aaaatactga tgataaggcc cgtgaccagc gccaccagca acagcggggc agccagtgcc
    11161 agcgcgactt tcatcgcttc agtccccatc atcatgaccg attcaggtgt cattttgcct
    11221 ctctagctgt aaaagctctg cgccagcgaa ccgaccagca attgccagcc atccaccagt
    11281 acaaacagca tcagtttaaa gggcagagca atggtggcgg ggggaccatc atcatcccca
    11341 atgccatcaa cacgctggca atcaccaggt cgataatcaa aaaaggaatg aaaatcgtga
    11401 agcctatctg aaatgcggtt ttcaactcgc tggtcacgta ggccgggcgc aaaatgcgca
    11461 tcggcacggc ttcaggtccc tgcaacgggc cggtattcgc tagtctggca aacaacccta
    11521 aatctgcctc acgggtctga cgcagcataa actcacgtag cggctgcgcc cctttttcca
    11581 gcgcctcctg cattgatatt ttctcttcgc tgaatggctg gtacgcatcg acataaattt
    11641 tgtcgatcac cggtgacata ataaaaaagg tcaaaaatag tgccagcccc agcaatacct
    11701 ggttaggtgg cgcggagggc gttcccagcg cgttacgcaa taaaccaaaa acaatgatga
    11761 tgcgggtgaa actggtcatc atcagtaaaa ttgccggaat aaacgtcaac gaggtgatga
    11821 acaccagcgt ctgcaccggg agcgaccagc tttgtccacc gccaggcagc ggctggctgg
    11881 tgatacccgg cagttgcgcg aaggcgaggg gcgtaaccag ccagagaagg acaggtgcga
    11941 cagacaacaa acgacgcatc aggatctccc actacgctta agcaaatttt tcatgaccga
    12001 ctgaaaatcg gtctgcggta tctcttctgt tggtgctgaa gggggaagtt tatgcagcag
    12061 attgatttgc cctgcggtaa cgccgagcac cagccgtgca tcttccacat cgaccaccac
    12121 aacccgttca cgcgcgccca gcgaagcact ggcgctaatt ttcagaccgt taacgccagt
    12181 gcgtttcggg gcaaatccca accgttttac ctgccaggca gcagcgagga tcagggcaat
    12241 aatggcgatg agtgcgccgc tgacctgcag cagtggcgca gcagaaaccg gcgtggaaga
    12301 ttgcacagta gcgtggttat tcatcactaa cggctcaggc gatgcattcg ctcagacgga
    12361 gtaatgatat cggtgatccg cacgccatat ttatcggcaa cgaccaccac ttcgccctgg
    12421 gcgattaaat aaccgttgat cagaatatcc agtggttcgc ccgccagacc gtccagcgcc
    12481 acgacggacc cttgcgtcag acgcaacagc tctttgatgg tcatccgcgt acggcccagc
    12541 tcgacggtca gcttgaccgg aatatccatt atcaggtcga tatcctgcaa cgttccgctg
    12601 acatcaccac cgccaaattg ctggaacacc gcgtcggcag cgcttttgct gctggttgat
    12661 ttttgttcgc tcaacgcttc agcccacaga tcgtccattg cgccgttgtt gtcatcggcc
    12721 ggattattca tgtcactcat ttgggctgtt cctcgttcag agaatttaaa atcgggttaa
    12781 tcaaatgttc tatccgtaac gcatactgac cgttgagagt gccgtactga ctggtcagca
    12841 ccgggacgcc gtcaacatgg gcgatgatgc gatcaggttt ttctatcggc aggacgtcgc
    12901 cgggtttcag ttttaaaatc tgcgacaggc gtagcgagat atcggcaaag ttggcgacca
    12961 gctccagctg tgaatgctgc acctggcgca ccaggttatc gcgccagttc tgatcttcat
    13021 tacgcgagtt ttccagcggc gggttaacca acaattcccg tagcggctcg atcatgctga
    13081 atggcagaca gatattaaat tcgccggtca ggttgccaat ctccacatgg aacggcgtgt
    13141 taaccacaat gtcgttcggc gaggtggtga tattggtaaa tttcacctgc atttccgaac
    13201 gcacgtactc aacttccagc ggattaatcg ccttccaggc gtcgctatag ccttcaagcg
    13261 ccagctttaa catgcggttg atgacgcgct gttcggtatg ggtaaactcg cgaccttcca
    13321 ctttggtcgg gaagcgtcca tcgccgccaa acaggttatc cacggcgata aacaccagac
    13381 tcggtgaaaa caccaccagc cctgtgccgc gcagcggttt cagatggata aggttcaggt
    13441 tggtcggcac cggcaggttg cgggcaaatt catggtacgg ctgaatgcgg atggccccga
    13501 cggttatatc cgggctacga cgcagcaggt tgaacagccc catacgaaaa tggcgggcaa
    13561 agcgctcatt aatgatttcc agcgcctgca aacgttcgcg cacaacccgt cgttgggtat
    13621 tcggatcgta cggacgaatg tcactttcgc cgctaacact ggctgtcggt tcgtctttga
    13681 cttcgctgtc accattcaac agcgcatcaa tttcagcttg agaaagaata ctatcgccca
    13741 tgtcgttatc gcagaataaa agcggtatac agcacgtcgg tgacatcctg tttcggttgc
    13801 ccggcaacaa gcggggtgga aagcgtggtt ttaatctcgg caatcaggtt tttcttgcct
    13861 tcttctgtcg ccagtacggc agcatcctga cgcgaaaaca gtaacagcaa acggctacgg
    13921 acttccggca aatactcact cagccgcgag cgggtagctt catctttcag gcgcagggtt
    13981 atgccgatat aaagcacgcg atccgcatcg cccaaattga ccgtgaaggt attcagcgcg
    14041 tagaagaccg gggagggtac gacgcgttgc tgcgctttgt cgtcagcggc aacctgatgc
    14101 gaatgccagt agctgtaacc tgcgctggca caggccgcga gggtaatgaa taccagaatc
    14161 gggatccaaa gcgatcgctt gcttttcttg cttatcgcgt aatcagtcat gtgttgcggt
    14221 cttcctgtgt cgctactgct tatcggctaa ttatcctgcg ttttgagcgc aacaaagcgg
    14281 ggaaaagacg cggattacgg tgctacctct gacgttaggc gaaaatatca acgccgctgt
    14341 tgcctgttac acgcccttgt aaagagacgg gaaccggaag cgtatcgtcg tcttcccccg
    14401 ccagaggttc atggtttact gtgcgttggc tttgctgttg ttgggaagcg gcctgctgct
    14461 gaccactaaa gctttcgcca ctgatgttgc tttgccctaa ctgaatgcca ctttcggcca
    14521 gctgcgtacg cagtaccgga agcgctgctt ccagcgcggc gcgtacatgc tgatgcggtg
    14581 aaaccatctg gatttgcgcc tggttatcat ccactttgag ggagatttgc acttcaccta
    14641 aatcctgcgg gtgcagacgc aactctgcac tttgttgccc ctggcgggtg aacagcgaaa
    14701 tatgctggct taatgattgt tgccattcgt gagaacccag cggtgcactc aaaacaggcg
    14761 cggcgacggt gggcagtggc tgtgtctggt gtggagtaat tagcgggctg gcggcagcgg
    14821 tcaccggtga cggtgtgctg atgacttccg ctttactctg ggcttctgct accagcggtg
    14881 ttaatggctg agctggcgtg ccgggggcat catcaggctg tgctgttgtg agttgcgcag
    14941 aagtcagttt tgtgaagagc gttggtttct ctgccggtaa cacggttgac ggcacatcag
    15001 tcactttggg cgtattgtca aaacccggca acatcgcaaa aagggcgctc aggcttgcgg
    15061 tgacgtcttc attcagatca tccgcttttt cgtcttttgt cgtattttgg tcagccaccg
    15121 cagccaacgt catcgtctga gcggtggtta acggtgttga tgtggattgt tcgtcgttga
    15181 tgacaggcag tgtttcatcc acggggatca gtaaatccgc ttgttgcgcg tcggcaagaa
    15241 tatcgctgat cagcggctcg ccttttgtcg tgggcttatc tgttgccacc agcaactggg
    15301 gggccgcttt gtcggtagtt gtctcgcctg ctaatgcttc gctcaacaac gcgagaaaat
    15361 cttgtgcagc atcgctggct ttgccgccag gcaatgtggt ggtgtcaacg tcggcggtaa
    15421 ttaagggcgc taagcgaatc attcaggttt cctcatggcg gcgcgctggg cgaactcatc
    15481 catctttttc tgatcgaggc ggttttctgc aagcagtgcc gccgtggata gccgttcctg
    15541 cagtgtctgc caggcctgca aacgttgttt cttttctcgc cagctgttca gggcaatgtc
    15601 aactttctgc gtccactgat taagttgctg gcgatgctga gtaatggctt tttccagcgt
    15661 ctggataaac tgctgatagt tgatccagcg gttgctggtc atcccggcac tcatatcgct
    15721 gttgaggtta ttgcgatatt cattctgata atcgatcagc attttgagct gttcttccgc
    15781 ctgctgacac ccgcgacgca tttcacccag caggcgcgcg gcatcctcta cctctttttc
    15841 tgccagatct ttcagggtcg ccagcgcgcc atgttctgcc atctgccgtt atctcctggg
    15901 ttatgacact gtcgggaaaa tacgctccag cccctggaga gacgcttccc agtccgcgcg
    15961 ttcaaaaatg ccttgttgca aatagccctc cagctgcggc cacagggcga tggctttgtc
    16021 gagcatcgga tcgctgcctt tggcatacgc gccgacgcta accagatcgc ggttacgctg
    16081 aaaactcgac aacagctgtt tgaaggtgcg cactcgcgcg taatgttgct cactgatcaa
    16141 cgccgtcatt gcgcggctga tcgacgcttc aatatcgata gccggatagt gcccggcttc
    16201 cgccagtcgg cgagacagca caatgtgacc gtcgaggatt gcccgcgcag agtcggcaat
    16261 ggggtcctgc tggtcatcgc cttcagtgag cacggtataa aacgcggtaa tcgatccgcc
    16321 gccgctaatg ccatttccgg cacgctcgac cagtgccggt aatttggcaa acaccgacgg
    16381 gggataacct ttagtggcgg gtggttcgcc aatcgccagc gcaatttcac gctgggccat
    16441 cgcgtagcgg gtgagggagt ccataatcag caacacatgc tgaccacgat cgcgaaaatc
    16501 ttcggcaatg cgcgtggcat aggcggcacc ctgcattcgc aggagaggag aaacatccgc
    16561 cggagcggca atcaccacgg agcgtgcacg cccttcggca ccgaggatat tctcaataaa
    16621 atcttttact tcgcgtccgc gttcaccaat caaacccacg acaatgacat cggcgcgggt
    16681 gtaacgtgcc atcatcccca gcagcacact tttaccaacg ccggacccgg caaacagccc
    16741 catacgctgc ccacgcccaa cggtaagcag ggcattgatt gggcgcacgc cggtgtccag
    16801 cacatgttca atcggtgtac gttgcaacgg gttaaatggc ggggtaatca gcgcaccggt
    16861 ttccgtcgta tcgggggagg ccatcgagcg gtttaccgct gccgtccaga acacgaccta
    16921 ataacgccgg accgagcggt aactgtttgc cgctttgcag cccttctgcc gaaatgtttt
    16981 tggcataaac acgcgcgccg ggcaggacac cttcgacttc ctccagcggc attaaaaaca
    17041 gccgttgacc gttaaagcca acgacttcac tttctacttc gtgcgtttca gtgccgttct
    17101 ggcgctcaat cacacaggtt gcgccgagcg gcaattgtaa tccggtggct tccagcacca
    17161 gcccggtagc gcgggttaat cgcccgtagc gacgtaccgc aggcaactgc gccattttgg
    17221 cttcaaagtt atccagcgtg gttagccagc gagtcaggcg cgtggtcatt acaccactcc
    17281 tggtgctgcc agacggcaga gttcttgcca gcgagtggcg acactggcgt cgagatcgcc
    17341 ttcatcggcg gagactttac agccgccagg atggagggtg ggatcgcccc gcaagcgcca
    17401 gccatgcaaa cttaaggtag cgccgagcat atcatccaca cgttgcagat catccgggtg
    17461 cacgcgcagc tgtggtttac cgctgaataa cggttcttgc tgcaacaact gttggatctg
    17521 tttgatgagt gccgagttat ccactgttgg cgtctgaccg atgacctgac gtgctgcctc
    17581 cagcgccatc tgcatcaggc gcgaagca
//
LOCUS       DAZPEB010000070        17601 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZPEB010000070 DAZPEB010000000
VERSION     DAZPEB010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17601
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.071"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(31..2478)
                     /gene="glgP"
                     /locus_tag="ACHURJ_002081"
     CDS             complement(31..2478)
                     /gene="glgP"
                     /locus_tag="ACHURJ_002081"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFR1281025.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2497..3930)
                     /gene="glgA"
                     /locus_tag="ACHURJ_002082"
     CDS             complement(2497..3930)
                     /gene="glgA"
                     /locus_tag="ACHURJ_002082"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFR1281026.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRASLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3930..5225)
                     /gene="glgC"
                     /locus_tag="ACHURJ_002083"
     CDS             complement(3930..5225)
                     /gene="glgC"
                     /locus_tag="ACHURJ_002083"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFR1281027.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLKAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5243..7216)
                     /gene="glgX"
                     /locus_tag="ACHURJ_002084"
     CDS             complement(5243..7216)
                     /gene="glgX"
                     /locus_tag="ACHURJ_002084"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFR1281028.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWDSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(7213..9399)
                     /gene="glgB"
                     /locus_tag="ACHURJ_002085"
     CDS             complement(7213..9399)
                     /gene="glgB"
                     /locus_tag="ACHURJ_002085"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFR1281029.1"
                     /translation="MSDRIDRDVINALIAGHFSDPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDASISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9672..10775)
                     /gene="asd"
                     /locus_tag="ACHURJ_002086"
     CDS             complement(9672..10775)
                     /gene="asd"
                     /locus_tag="ACHURJ_002086"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFR1281030.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVVDELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10967..11560
                     /gene="yhgN"
                     /locus_tag="ACHURJ_002087"
     CDS             10967..11560
                     /gene="yhgN"
                     /locus_tag="ACHURJ_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFR1281031.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11617..12957)
                     /gene="gntU"
                     /locus_tag="ACHURJ_002088"
     CDS             complement(11617..12957)
                     /gene="gntU"
                     /locus_tag="ACHURJ_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFR1281032.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGPLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12961..13488)
                     /gene="gntK"
                     /locus_tag="ACHURJ_002089"
     CDS             complement(12961..13488)
                     /gene="gntK"
                     /locus_tag="ACHURJ_002089"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFR1281033.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMLRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13627..14622)
                     /gene="gntR"
                     /locus_tag="ACHURJ_002090"
     CDS             complement(13627..14622)
                     /gene="gntR"
                     /locus_tag="ACHURJ_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFR1281034.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVSYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14846..15541)
                     /gene="yhhW"
                     /locus_tag="ACHURJ_002091"
     CDS             complement(14846..15541)
                     /gene="yhhW"
                     /locus_tag="ACHURJ_002091"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFR1281035.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15664..16701)
                     /locus_tag="ACHURJ_002092"
     CDS             complement(15664..16701)
                     /locus_tag="ACHURJ_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFR1281036.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFISDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLSVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     PEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17033..17521
                     /gene="yhhY"
                     /locus_tag="ACHURJ_002093"
     CDS             17033..17521
                     /gene="yhhY"
                     /locus_tag="ACHURJ_002093"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFR1281037.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
BASE COUNT         4340 a         4795 c         4384 g         4082 t
ORIGIN      
        1 cctttggccc cgttctattt attggtgaac ttacaatctc accggatcga tatgccagat
       61 atgatcggcg tactctttga tagtacggtc agaagagaag tagcccatat tggcaatgtt
      121 cagcatcgct tttgcggtcc actcttcctg aagctcgtag agttcgtcga ctttatcctg
      181 acaatcgaca tagctgcgat aatccgccag aacctgatag tgatcgccga agttgatcag
      241 cgaatcaacc agatcgcgat agcgacccgg atcttccgga ctgaatacac cgctgccgat
      301 ttgcgtcagc acctgatgca gctcctcatc tttctcgtag tattcacgcg gtttgtagcc
      361 ctgacgacgc agttcttcca cttcttccgc tgtgttacca aaaataaaga tattgtcagc
      421 accgacatga tccagcatct cgacattcgc accgtccaac gtaccgatag tcagcgcacc
      481 gttaagcgca aacttcatgt tactggtgcc ggaagcttcc gtccctgcca gcgaaatctg
      541 ttcagacaga tctgccgccg gaatgatcaa ctgcgccagg ctaacgctgt agttcgggat
      601 gaacacgact ttcagcttat cgccaatctg cggatcgttg ttgatcactt tcgctacgtc
      661 attgatcaaa tgaataatgt gcttcgccat gtaataggcc gaagccgcct taccgccaaa
      721 aatattcacg cgcggtaccc acttcgcatc cgggtcggcc ttgatgcggt tatagcgggt
      781 aatcacatgc aacacattca tcaattgacg tttgtattcg tgaatacgtt tgatttgtac
      841 atcgaacaac gcctttggat tcaccaccac attcagctgc tgggcgatat actctgccag
      901 acgctttttg ttctccagct tcgcctgatg cacagcgtga ttaaccattg ggaaatcaca
      961 gtgttgttgc agctcattaa gcaggctaag gtcggtgcgc cagttacggc ccaggtgttc
     1021 gtccagcacg gctgaaagcg atgggttcgc taccgctagc cagcgacgcg gcgtcacacc
     1081 gttggtgacg ttggtgaaac gacccgggaa gattttcgca aagtcggcaa acaacgattg
     1141 caccatcaga ttagagtgca gttccgatac accgttaact ttgtggctca caacaaccgc
     1201 cagccaggcc atacgcacac gacgaccgtt ggattcatca atgatcgacg cccgtcccag
     1261 cagatcggta tcgttcggat actgttcctg caaggttttc aggaaatagt cgttgatttc
     1321 aaagatgatc tgcaggtgac gcggcagaat tttacccagc atatcaaccg gccaggtttc
     1381 cagcgcctcg ctcatcagcg tgtggttagt gtaggagaag acctgacaac acacctcaaa
     1441 cgcgtcgtcc cagctaaatt ggtgctcatc gatcagcaga cgcatcatct caggaatcga
     1501 cagtaccgga tgggtatcat tgagatgaat cgcgatttta tccgccaggt tatcgtaggt
     1561 tttatgcaac tgataatggc ggcttaaaat gtcctgaatg gtcgaggaaa ccagaaagta
     1621 ttcctgacgc aggcgcagct cacgcccgga gtaggtggag tcatccggat acagtacgcg
     1681 agatacgttc tcggagtggt ttttatcttc cactgccgcg aagtagtcac cttggttgaa
     1741 tttaccgagg ttaatttcgc tactggcttg cgcactccac aaacgcaacg tgttggtcgc
     1801 gtcggtgtcg taaccaggga ttatctgatc gtaagcgact cccagaatct cttcggtttc
     1861 aatccagcgc gtttttttac cttcctgctg aatgcgacca ccaaaacgga ctttatagcg
     1921 cgtgttgtgg cgtttgaatt cccacgggtt accgtattcc agccagtagt ctggcgactc
     1981 tttctggcta ccgttaacga tgttctgctt gaacataccg tagtcatagc ggatgccgta
     2041 accgcgcccc ggcaacccta acgtcgccag agaatcaagg aagcaagccg ccagacgtcc
     2101 caggccaccg ttaccgaggc ctgggtcatt ttcttcatca atcagctctt cgagatttaa
     2161 ccccatcgct tccagtgcgc cctgtacatc ttcgtaaatt cctagcgaca acatggcgtt
     2221 ggagagcgta cggccaatca aaaactccat cgacaggtag taaacctgac gagtttcttg
     2281 cgacaactgg gcacggtttg aacgtaacca gcgctccacg agacgatcgc gcacagcaaa
     2341 taacgttgcg ttcagccatt catgtttatt ggcgacgacc gggtcctttc caatcgtaaa
     2401 catcagcttg taagcgatag agtgcttaag agcttctacg ctaagcgtgg gcgatgaata
     2461 tgtaaacgga gcattcatat aggcgtttcc tgaaaactat ttcaagcgat agtaaagctc
     2521 acggtacgac ttcgccgcga cctgccagct aaaatccatt gccatagcct gacgttgcac
     2581 aaaccgccac agtgaagcac gagaccacag tacaaaagca cgtcgaatag cccgtaacag
     2641 cgaccaggca ttactatctt caaagacaaa cccactggcg acgccatctg caaggttctc
     2701 aagagaacag tcagaaaccg tatcagcaag cccaccggtg cgccgcacta acggcagcgt
     2761 accgtacttc aatccataaa gttgcgttaa gccgcacggt tcaaaacggc tgggcaccag
     2821 aatgacgtcc gcgccgccca taatgcgatg cgaaaatgct tcgtgatagc caatctgaac
     2881 gcccacctgg ccggggtatt ccgctgccgc cgcaaggaaa ccttcctgca gcaccggatc
     2941 gcccgcgccg agtagcgcca gctgcccgcc ctgctccaga agacccggta aggcttccag
     3001 caccaggtcg agacctttct ggctggtcag acggctcacc actgcaaaaa gcggcacttt
     3061 atcgtcaacc ttaagcccca ttgcgatttg taactggcgc ttattttccg ctttatcttc
     3121 caacgtatcg cgggtgtaac gcgaggccaa cagtaagtcc gtctctggac tccagatttt
     3181 ctcgtccacg ccgttcagta cgccggaaag acgcccttca cggtgacgct gttgcaacag
     3241 accttccata ccgtaggcaa actgcggttc ggtgatctcg cgagcgtagg ttggactgac
     3301 cgccgtaatg tgatcggcat agtacagacc ggccttcagg aaagagattt gtccgttgaa
     3361 ttccagccca tgaatattaa agaatgacca tggcaattgg atgtcattca tgtgatgtgc
     3421 ataaaacatg ccttgatagg ccaagttgtg cacagtaaac accgacttcg ccggacgccc
     3481 gcgcgccgcc agatacgcag gcgcaaggcc tgcatgccag tcgtgcgcat gcaccacatc
     3541 aggacgccag aatgggtcaa gcccgctggc catttctgcc ccaacccacc ccagcagcgc
     3601 aaaacgcaat acgttgtcgg tataggcaaa taagttggta tcgtgatacg ggcttcccgg
     3661 acgatcatag agatgcggcg cgtcaatcag gtatatgcca accccgttgt aatgaccgaa
     3721 caacagcgtg atatgtccgg cgaaggtatc acgacgggat actacctgcg catcggtcac
     3781 gccacggcga atatcgggaa atgcaggcaa cagtacgcga gcgtcaacgc cgtctgcgat
     3841 ttgtgctgcg ggtaatgccc caataacatc agccagaccg ccggttttaa gcagcgggaa
     3901 catctctgaa catacatgta aaacttgcat tatcgctcct gtttatgccc taacttccgt
     3961 agcatttcgc gcgttaccag cacgatgcct tcttctgaac gatagaaacg acgtgcatct
     4021 tcctctgcgt tttcaccaat caccatgcct tccgggataa cacaagcacg atcgatgacg
     4081 cagcggcgca gacggcacga gcgacctacc catacttccg gtaacaatac ggcggaatca
     4141 atgttgcaga atgaattcac gcgaacgcgc gagaacagaa cggactgcac caccaccgaa
     4201 ccggagatca cacaaccgcc ggataccagt gagttaaggg tcatcccgtg gctaccggag
     4261 cgatcctgca cgaatttcgc tggcggtaat gattcattgt aggtgcgaat tggccaattg
     4321 cgatcgtaca tatccagttc cggcaccaca gaggccagat cgagattcgc tttccagtaa
     4381 gctttcagcg tacccacatc gcgccagtac ggctcggcat ccgggtcgga ttgtacgcaa
     4441 gagagcggga acgggtgcgc ataggccaga ccggcttcgg tgatcttggg aatcaaatct
     4501 ttgccaaagt cgtggctgga gttctcatca cgatcgtctt cttccagcag ttcatacaga
     4561 tagtcggcgt caaagacgta gatacccata ctcgccagag atttgctcgg atcgttcggc
     4621 attgacggtg ggttagcagg tttttcaacg aattcgataa ttttatcgtt ctcatcaacc
     4681 gccataacgc caaatgcgga ggcttcttca atcggtactg gcatacaagc aacggtgcaa
     4741 cgtgcgcctt tttcgacgtg atcgataagc atacgcgagt agtcttgctt gtagatatgg
     4801 tcgcccgcca ggatcaccac gtattccgct ttataacggc ggataatgtc gaggttttgg
     4861 gtgaccgcat ctgcggtgcc gcgataccag ttttcccctt tcattctctg ctgtgctggc
     4921 agcagatcga caaactcgtt catttcttca ttgaagaatg accagccgcg ctgaatgtgc
     4981 tgcaccagag tgtgggactg gtactgggtg atcacgccca tacgacggat cccggagttg
     5041 atgcagttag acagcgcaaa gtcgataatg cggaacttac cgccgaagtg tacggccggt
     5101 tttgctcgct tattggttaa atccttcagg cgggtaccac gtcctcccgc cagtatcagg
     5161 gcaacagatt tcaatggcag ctggcgcgcc aacattaagt gatcgttctt ctctaaacta
     5221 accatgacta actccttttt tatcatctct ggaacacaca caatccgtgt gcaggtccct
     5281 gccagacagc cgtaatcact gggttatcct ctccagcgaa tgggggaatg gcgtgccact
     5341 ccccagcagg taaaacaatc tctgttacct caagcgtggc gttaattgcg atcaaaaagc
     5401 gatccgagag cagaatttgc agctgtttcg gcccgttttg ccactcatcc gtgcttaaag
     5461 gttgagcata tcgatttagc caacggacat tgccatcgcc ttcttcccac cagcgattct
     5521 ccaccaaagc gggaatgcgc ttgcgcagat ggattaacgc ggcggtaaat gcggttaaac
     5581 cactgcttgc ctgcgaccag tccaaccagg ttaattggtt atcctgacag taggcattgt
     5641 tattgccatg ctggctgtga ccatgttcgt caccggccag taacatcggc gtaccctggg
     5701 agagcaacaa cgttgttaac aaggcgtgaa tactgtcgcg ccgccgttca accaggtcaa
     5761 gagaaccgcc taacccttct ttaccatgat tgttactgta attgttgttg gtcccgtcgc
     5821 gattttcttc tccgtttgct tcattgtgtt tatggttgaa gcaaacgcag tcgcgaagcg
     5881 taaaaccgtc atgcgcagtg acgagattaa tcgcggcact cggcagacga ccattacgtt
     5941 taaaaacatc gctggaggca gcaaaacgcc cggcaaacgc ccccagaggc aaatcataat
     6001 gtagccagaa acgacgggca gcatcgcgga aatgatcgtt ccactcggca aacagcggcg
     6061 ggaaatttcc cacctgataa ccaccagggg cgatatccca cggttcagca attaacttca
     6121 cctgcgagag caccgggcag ttctggatag cggtaaacaa cggcgcatcc tgacggaact
     6181 ctggcgtacg gcccatgact gccgccagat caaagcggaa accatcgacg tgacaggttt
     6241 ctacccaata acgcaggcag gcgctggcat aatccaccac cgccggatga ctcaaattga
     6301 gcgtgttgcc gcaaccggtc cagttgtgat aatcgccgtc ttctcttatc caataatagc
     6361 tacggttatc gatcccacgc agtgagaata acgggccgtc gaggtccagt tccgcactat
     6421 ggttgagcac gatatcaaga atgacttcga tacccgcttt atgcagtgct ttgattgcat
     6481 cgcgaaactc atccagcgcc gtttctggcg agcaggcata cgccggatgc agcgcaaaca
     6541 tcgccaccgg gttgtaaccc cagtagttac ttagccccat gcgttgcaga cgtggttcac
     6601 tggcaaactg cgccactggc agcagttcca gcgcggtaat gcccaattgt ttcaaatagt
     6661 tgatcatcac cggatgcccg agggctttat aagtgccacg gatctcgacc ggaatctccg
     6721 ggtgcaagta cgttaatcct ttgacatggg cttcataaat gatggtgctg tcccacggcg
     6781 tgcgcggcgg ggcatcatct tcccagtcat agtgatcaac caccactacg catttcggcg
     6841 caatggcggc gttgtcgcga tagtcaggtt cattatgacc ggcgtgcagc agcgggttat
     6901 ctttaaattc cccgtcaatt tgccgcgcgc aaggatcaat caacaacttc gccgggttaa
     6961 agcgatgccc ctcggcgggt tgccaggggc catgaacgcg ataaccataa cgcaaacccg
     7021 ggcgcgcatc cggcagataa ccgtgccaaa tgtcgccact gtgccctggc aagtcatagc
     7081 gatgttcctg gccattggcg tcaaagacac acagttctac ccgctcggca tgagcggaga
     7141 aaagtgtgaa gttgacgccc tgaccgtcgt aatgcgcgcc gaggggagcg ggtttgccaa
     7201 tggcgagttg tgtcattctg cctcccgaac cagccagata gtggccagcg gtggtagtgt
     7261 caggcttagt gaatgctgac gaccgtggct ggcaatctca tcgctgtgta ccgtgccgcc
     7321 attgcctgca ttactgccgt gatagtgcat ggaatcggta ttgaggattt cacgccattt
     7381 gcctggctga ttaatgccga agcgataatc atgacgcggc accggcgtaa agttactggc
     7441 aacgataatt tcgttaccct ctttatcgcg acgcacaaag atcagcaccg agcgttcttt
     7501 gtcatccacc accagccatt caaagccgta cgggtcaaaa tccagttcat gcattgcttt
     7561 atggtggcgg taggtgaggt tcagatcgcg caccagacgc tggacaccgt ggtgccagtt
     7621 atcgccacct tccaacagat gccagtcgag gctggcgtca tggttccact cacggccctg
     7681 ggcaaattcg ttccccatga acagcagttt cttgcccggg aatgcccaca tccagccata
     7741 gtaggcgcgc aggttagcga atttctgcca tgcgtcgccc ggcatgcggt cgagaatcga
     7801 ttttttaccg tggaccactt catcatgcga caacggcagg acgaagtttt cagtgtagtt
     7861 gtagagaatc ccgaaggtga gtttatcgtg atgatactga cgataaaccg ggtcgagctt
     7921 catgtagtcc agggtgtcat gcatccagcc gaggttccac ttgtaccaga agcccagacc
     7981 gcccatatcc tgcggacgag aaacgccagg gaaatcggta gactcctcag ccattgtcac
     8041 cgcaccggaa acctgctcac caagaatacg gttggtatta cgcaagaatt caatcgcttc
     8101 aagattctcg cgcccgccaa attcgttcgg gatccactcc ccctctttac ggctgtagtc
     8161 gcgataaatc attgacgcca ccgcatcgac gcgcagcgca tcaataccaa aacgttcaat
     8221 ccagtaaagc gcgttaccga cgaggaagtt actgacttca cggcgaccat aattgtagat
     8281 cagcgtattc cagtcctgat gatagccttc gcgcggatcg ctgtgttcat acaagttcgt
     8341 gccatcaaat tcggcaagcg caaagtcatc ggtcgggaag tggcctggca cccagtcgag
     8401 aatcacgttc agacccgctg cgtgtgcggc atcaatgaaa taacggaagt cgtcgcgagt
     8461 accaaaacgg cgggttggtg catacaggcc ggttggctga taaccccaac tgccatcgaa
     8521 gggatgctcg ttaatgggca gtagttcgag gtgggtaaag cccatccatt tagcataagg
     8581 caccagttga tcggccagct cgcggtagct caaccagaaa ttgttatcgg tgtggcgacg
     8641 ccaggagccc aggtgaactt cataaataga gattgacgca tcaaactgat tcgctttttt
     8701 gcgctcttca gtctgtacaa ccttttccgg cagcccgcaa ataagagacg cggtttccgg
     8761 gcgcatttgc gcttcgaagg cataagggtc ggacttcaga cgcaagttgc cattggcatc
     8821 aatcatctcg tatttataga gctgaccgtt atgcgcccca gggataaaca gttcccaaat
     8881 gccgctctct ttacgcagac gcatcgggtg acggcgaccg tcccagtagt tgaattgccc
     8941 aaccaccgag acccgacggg cgtttggagc ccaaacagag aaacgcgtac ctgtgacgcc
     9001 atccatagta tctgcatgcg cgcctaaggt ttcatacggg cgcaggtgag taccttcaga
     9061 taatagccag gcatccattt cctggattag cgggccaaaa cggtaaggat catcaatcag
     9121 gttttgctga ccatgccaga caacagccaa ctgatagcgg aaaaaattct tacgtcgcgg
     9181 aatgacaccg ctaaagaatc cccgtgagtc aagacactcc agttttgcga gtttgcgccc
     9241 ggttttcggt tcaatcaccc acacatcggt agcgtcgggt aaaagggcac ggacttccag
     9301 tcccgcggtg gttttatgca ttcccagtac ggaaaaagga tccgaaaaat ggcctgcaat
     9361 tagcgcgtta atcacgtctc tatcgatacg atcggacatg cttgtcttcc tgttttattg
     9421 tgtcacccca tccaattgga tttttgactt ctggttgtga catttttttg acctgaacgg
     9481 cgcagcactc tgtgcatcct ctctgcgtcg tcctcacttc aggtaaggct gtgaatactc
     9541 atgtattcag ccacccttaa agaatagcca atgctctatt taactcccgg taaatcatga
     9601 aacatctgcg cttactcctg tattacgcac taacaggggc ggcatcgcgc cccagattta
     9661 atgaataaag attacgccag ttgacgaagc atccgacgca gcggctccgc ggccccccac
     9721 agcagctggt cacccacggt aaaggctgac aggaactccg gtcccatatt cagcttacgc
     9781 aggcggccta ccggcgtggt cagcgtgccg gtaacggcag ctggggttag ctcacgcata
     9841 gtgatttccc gatcgttcgg aacgactttc gcccacggat tgtgcgcagc cagcagttct
     9901 tcgacggtcg gaatagacac atcttttttc aatttaatag tgaatgcctg gctgtggcag
     9961 cgcaatgccc cgacacgcac acataaacca tctaccggaa ttacggaaga tgtgttgagg
    10021 atcttgttgg tttccgcctg ccctttccac tcttcgcggc tctgaccgtt atcgagctgt
    10081 ttgtcgatcc acggaatcag gctacccgcc agcggcacgc caaagttatc caccggcagc
    10141 tcaccgctac gggttaaggt tgtgactttg cgttcgatat cgagaatagc agaggacggg
    10201 gtcgcgagtt catctaccac atggccatac agatggccca tctgggttaa taactcacgc
    10261 atatgtcgcg caccaccgcc ggaagcggcc tggtaggttg caacggacac ccaatcaaca
    10321 agatcattgg cgaataaacc acccaacgac atcaacatca ggcttacggt acagttaccg
    10381 ccaacaaaag tcctgatgcc attatttaat ccgtcggtaa tgacgtcctg attgacgggg
    10441 tcaagaatga tgatggcgtc atctttcatg cgcagagacg atgctgcgtc aatccagtaa
    10501 ccttgccatc cgctttcacg aagctttgga tagatttcgt tggtataatc gccgccctga
    10561 caggtcacaa tgatatcgag ggcctttagc gcctccagat caaaggcatc ctgaagtgtg
    10621 ccagtggttc cgccaaaaga cggcgcagcc tggccaagct gagaagtaga aaagaagaca
    10681 gggcgaatgg cgtcgaagtc gcgctcttca accatgcgtt gcatgagaac ggagccgacc
    10741 ataccgcgcc agccgataaa accaacattt ttcataagcg ttttttcctg caaagatgtg
    10801 tgctgtataa atgtgccggt ctcctcttgg cacatctttc accatacaaa aagcagccaa
    10861 agtcgcaagt gaaattaatc aatgatagcg aagccatcag taatgcgact tatcctgctt
    10921 tgttagcaca cagaaagtcc gcggcaatta tcagggaatt tgagttatga atgaaatcat
    10981 ttctgcagca gttttattga tcctgattat ggatccgctc ggaaacctgc ctattttcat
    11041 gtccgtactg aaacatactg aaccgaaaag acggcgggca atcatggtgc gagagttgct
    11101 tattgctctc ctggtgatgc tggtgttcct gtttgcgggt gagaaaattc tggcatttct
    11161 cagcctgcga gcagaaaccg tctccatttc tggcggcatc attctgtttc taatcgccat
    11221 taagatgatt ttccccagcg cttcaggaaa tagcagcggg cttccggcag gtgaagagcc
    11281 atttatcgtg ccgttggcaa ttccgttagt cgccgggccg actattctcg ccacgctgat
    11341 gttgttgtct catcagtacc cgaatcagat gggacatctg gtgattgctc tgctgctggc
    11401 ctggggcggc acctttgtca tcctgctaca gtcttcgcta tttttacgtc tgctgggcga
    11461 gaaaggggtg aacgcacttg aacgcctgat gggattgatt ctggtgatga tggcaaccca
    11521 gatgttcctc gacggcattc gaatgtggat gaaggggtaa cgaggagagt ttttcgaaaa
    11581 gaacccggta gcgtcacaac taccgggcga acaaacttaa ctcaacagct ggaacgcaat
    11641 catcccaacg atggcaccaa cagtgccgag gatggtttcc atcatggtcc aggttttcag
    11701 cgtttcggct tcggtcgcgc cggtaaattt accgaacaac cagaaaccgg cgtcgttaac
    11761 gtggctgaca acaatcgaac caccagcgat acaaatcgac agtgccgcca tttgcgcacc
    11821 ggagtagttc agttgttcaa tgaccggcat caccagcccc accgccgtta aacaggcaac
    11881 ggtggcagac ccctgaatga tgcgcactgc agcggccagc acgaagcagg tgatagcaat
    11941 cggcaggccc atgccggtta acgcttcgcc cagtgccgga cctacgccag agtcaaccag
    12001 cacctgtttg aacacgccgc ctgcaccaat caccagcaga atgatccccg ccggttgtag
    12061 cgcgtgaccg caaatctcca tcactttgtc ttttggcatg ccctgacgca ttgccaggcc
    12121 ataaatcgct accagacaag caaccagaat cgcggtaaat ggatgaccaa taaactcgaa
    12181 ccattcgtaa gcagttgatc cttctggcac aaaacgagcg gcaatggttt tcagccccac
    12241 cagcaccagc ggcaacagga tcagcgacag gctgaatccg aaggacggca ttttgccttc
    12301 gccgagatgc ggttcgctga tgtcgtcagg aatatgcaac tccacgtaac ggctgatgaa
    12361 attaccccac agcggcccgg caataatcat tcccggaatt gccgcacaca gaccaatcag
    12421 gatcatccag ccaaagtcgg cattcatctg cgatgccagc aacatcggcg ctggtccagg
    12481 caccagaaat gccgccgctg ccgccacgcc tgcgaataat gggattacca gcttcaccag
    12541 gttcgtaccg gtgtggcgcg ccattgagaa agcaacgcta atcagcagaa ctatcgccac
    12601 ttcaaagaac agcggtagcg cacagaccaa ccccgcaagg ccgatggcat aatgcgcgcg
    12661 gctgtgaccg aaggatttga gcattttgac ggcaatctga tcgactgcgc cggtttcatg
    12721 taagatcttg ccaaacatag ctcccagggc gacaaccacc gccaggaagc cgagggggcc
    12781 tcccatccct ttttccatcg tcgctgcgat tttatcgagc ggcataccgg aaaaaaggcc
    12841 agcccccatg gacaccacca tcaaagccag gaaagcgtgc atacgcgcct tcatgactaa
    12901 aaacagcagc agtaaaacag accctactgc tgttaaaaca agcgttaatg tagtcactac
    12961 ttatttgcct tttttaataa cctcaatggt gcttgccaca acaccttcca gcggttgatc
    13021 gatatccacc accagtacat cggtttcgtc cgcacccggc tcctgcagcg tttcaaactg
    13081 cgtcaccaac atttgggttt taaagaaatg gcctttgcgc gctttcaagc gactttcaat
    13141 cacatcaaaa tcgcctttca ggtagatgaa agagagattc ggattacctt cacgcagcaa
    13201 gtcgcgatag tgctttttca gtgcagaaca gacgatcagc gacactttat tggtgcgcag
    13261 catagcaaac gcggcgtcgt tcagcgcctg caaccagggt ttgcgatcgt cgtcattcag
    13321 cggttcgcca gacgccattt tttcgatatt gcgccgtgga tggaggaaat caccatcaag
    13381 aaacgcggca tgaagttgat gcgccacttc actggcgacc gcagatttgc cgctgcccga
    13441 tacgcccatc aagacgtaaa tgtggtgatc atggttagtc gtgctcaaag tggtgccccc
    13501 acaatacaag aattaaaaat gttacgggta actgttatcg gtaacattgt ccagccggac
    13561 aatatcagaa gcaatatcca tccgtgcctt aagtgtataa gtgtgagcta cttcaaattt
    13621 gtaggcttaa atagatccgc ccggtgacaa ggtgaaacct aaatctaaca ttttcggtgt
    13681 cacagattcg ccacgaatac gcgccagcag gcgttcagcg ccaatactgc ccatccgctc
    13741 acgcggcgtc agcacgctcg ctaaccgcgg ctccatcacc tggccaatgt catgaccgtg
    13801 gaaaccggca atcgccatat cgtcaggaac cttcaatccc agacgctgac attcaaacgc
    13861 cgcgccgacc gccaggtcat cgttggtaca gaacacgcca tccagctgcg gatattcccg
    13921 ccgcgcctgg cgaatcagtt caataccgga agagtaagaa gaagattgct caaccatcac
    13981 gctatacgac accagacctg catccagcat cgcctgttcg tatcccttct gtttgatgat
    14041 agtacgttcg tcgagacgcg cgccgagata ggcaatgtgg caatgcccgc gagcaataat
    14101 ggcggtggtc atctggcgtg ccgcttcaaa gttatcaaaa ccgacggcga tatcgaggca
    14161 cggcgactgg ctgtccatga gttccaccac cgggatcccc gccacttcaa tcatctttaa
    14221 ggtgcgcggc gtgtgggtac gttcggtgag gatcagtccg tcgatattcc atgagagcat
    14281 ggattcgaga cgttcttgct ccatttccgg tttataaccg tagtgagcca gcatggtctg
    14341 ataaccgtgc gcgtcggtga cgctttcgat tccgcgtaat acttccgcga aaacctggtt
    14401 ggtgagagaa ggtaacagga cgccaatcgc ccggctggtg gcgttagaga ggatatcggg
    14461 cgcacgattg ggaatatagc ccagttcatc aagagcggcg gcaatcttgc cgcgtagagc
    14521 gacggaaacc tgctccgggt tgcgtaaaaa acggctgacc gtcattttgg tcacgcctac
    14581 acggtcagcc acatcctgaa gtacgggtct tttctttttc atcgtcctga aggtacaaaa
    14641 gagatagatt ctcttagttt aacacggacg catcagaacc ttcccgggga aaacagtgct
    14701 tgtttacaat tattagatat acactaaatc aattgcatcg cattgtgcta acgacgcggc
    14761 aaaaccccgg aagcgtgcat cagcacgcga ccggagcgag cgaggaagcc tgcaaaaagg
    14821 caggcttcaa agatgccgtt gagttttaaa ccggcggcag atcgaacagt aacacttcgc
    14881 tgtcgctatc cgcatggatg gagattgcct gctcatccca gattgccaga ccatcgctgg
    14941 tcgaggcttt cacgccgtta atggtgacat tgcctttcac cacctggatc cagacgcggc
    15001 gttcagcggc aatctgatgc accgactgct catctttcag caacgcccag cggtacagtt
    15061 ccatatcctg atgcactttc aatgaaccat ctcgcgcatc cggcgagagc accagctgtt
    15121 tgccctgtac ggcatcgaag cgacgctgtt cataacgcgg cgtaataccg ttttcttcag
    15181 gcatgatcca gatctgatac agatgcagac gctcggtgct gcttgggttg tactctgagt
    15241 gacgaatacc cgtacccgca ctcataatct ggaactcacc cgccggaacc tgctctttat
    15301 tgcccatgct gtcctgatgc tcaacagtac cttccagcac gtaggtcaaa atttccatat
    15361 ctttatgcgg gtgagtgccg aagccctgcc ctgcttcaat cacgtcgtcg ttaatcacgc
    15421 gcagcgcgga gaagcccata aagttcggat cgtaatagtt ggcaaaagag aaagtatgcc
    15481 aggagtccag ccagccatga tttgcatgac cacgttcatt tgctttgcgt aagtagatca
    15541 tttgtattcg ccccctgaat gatttcgatg ggcttagtgt ggacccgatc tgcctgggat
    15601 gatagagggt gaaaattgcc ccctctgttc aaaaaaattg aactattcga ggggccatca
    15661 aacttaattc gcgagagtca ctgtggaggg agaggcttgc tcaaatccgc gttcaaggat
    15721 ttccagattg gtaagaactt cagattcctt gacgtaattt ggcgcaccgt gggtgatggt
    15781 ttgatacaac gcatcataaa cgcgcccgta atcgcccatc tccggcttca tctcttctct
    15841 gaccgtcacg ccctcgtcat tgacatactc cggcacaccg accgaatcat ccgctgcgaa
    15901 tcccggttcg cccggcataa tattagcctt caggctggtt tcctgctggt cgataccgta
    15961 tttaataaac gaacctttct taccgtgaac gataaatttc ggataatcga ttttcaccag
    16021 atggctggtt ttgacgatgg ctttcaggtc gccataaaac agttgcgctt caaaggtgtc
    16081 gtcaggattg gctttattac gcaggctgcg gatgtcataa gcgacgtgat ccgggcgacc
    16141 gaacagagaa ataatctggt ccatcgtatg cacaccaagg ccataaaacg cgccatcctg
    16201 cggcagccca ggtttggttt ctgccaccgg gcggtaatag tcaaaatggc tttccacttc
    16261 aacaatctct cccaacttgc cactttcaat cgcttttttc gctgtcagga agcaggagtc
    16321 aaagcgacga ttctgatacg gcgtgacgct cagccctttg cttttcgcca acgcaaacag
    16381 ctccttcgcc tgggcaagcg tcggggtgaa cggtttttcg accagcacat ttttcccggc
    16441 ttccagcgcg cgtttcgcgt actcgaaatg gctgtccgcg tgggtgcaga caacaaccag
    16501 cttaacatcg ggatcgttta gtacttcgtc gagatcgctg atgaaatgga tatgggaata
    16561 aatgggagcc tgttcttccg gcttcgcatg gcgacgaaaa atatgcgcga catgccagct
    16621 atccttgcgg ttaagtacat acggcagatg gtaacgggtg gtgcttttgc cgaagccaat
    16681 aaaggcgcag ttgatgacca tgatttcgtc ctttttaagg tggttattta caccttagcg
    16741 caaagcggac gtggttccta ctggagtgcg cataacgaac acaagcactc ccgtggataa
    16801 attgagaacg aaagatcaaa aaaaagccag cacccggctg gctaagtaat actggaagca
    16861 atgtgagcaa tgtcgtgctt tcaggttctc cgcgagggtc ttcctgatcg cgagacaata
    16921 ataatcattc tcattcgcac ttgtccaaca ctttttgcaa aaaaatgcat ttgactcgca
    16981 tttgaaagtc aatgatgttg aaagggacat ttaccccaaa gaggacaaag gaatgagtga
    17041 gatagtaata cgccacgcag aaacacggga ttacgaggcc atcaggcaga ttcacgccca
    17101 gccggaggtg tattgcaaca cactacaggt gcctcatcct tccgatcata tgtggcagga
    17161 gcgactcgcc gatcgtcccg gcatcaagca actcgtcgcc tgtattgatg gagacgtcgt
    17221 gggccatctc accattgacg tgcaacagcg cccacgccgc agtcatgttg ccgattttgg
    17281 tatctgtgtc gactctcgtt ggaagaaccg cggcgtcgcc agcgccctga tgcgagagat
    17341 gattgaaatg tgcgacaact ggttgcgggt agatcgcatt gaactaaccg tgtttgtcga
    17401 taacgccccg gcaattaagg tctataaaaa atacggcttt gaaattgaag ggactggtaa
    17461 gaagtacgca ttgcgtaatg gtgaatatgt cgatgcatat tatatggcgc gggtgaagta
    17521 agatagtgcc ctttttctga gatggaaaaa gggtgtcatt caaaatcgac ataccttcct
    17581 ttaaggtatt tatttaccca a
//
LOCUS       DAZPEB010000071        17503 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZPEB010000071 DAZPEB010000000
VERSION     DAZPEB010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17503
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.072"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..744
                     /locus_tag="ACHURJ_002094"
     CDS             <1..744
                     /locus_tag="ACHURJ_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFR1281038.1"
                     /translation="SSTDKPAKLKFHQYARATLLSNRGRDHNCDGRRTVEIHKLDLSD
                     WQAFNKLATKCNAYDGVTMNGDNSFGWNHEATLDNIHAQKYNKAYAGARLTAELKYLL
                     QDVESFEPNSKYTIHEVVLGPGYGTPDYTGQTIGYVVTLPAQMPNCWSSELPTIDLYI
                     DQLHTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNSAAIYSGLGFERVPQDRD
                     IRSDRMHLTPANHPDLWTQENGEWIYLRN"
     gene            complement(815..1264)
                     /gene="rplI"
                     /locus_tag="ACHURJ_002095"
     CDS             complement(815..1264)
                     /gene="rplI"
                     /locus_tag="ACHURJ_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFR1281039.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(1306..1533)
                     /gene="rpsR"
                     /locus_tag="ACHURJ_002096"
     CDS             complement(1306..1533)
                     /gene="rpsR"
                     /locus_tag="ACHURJ_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFR1281040.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            complement(1538..1852)
                     /gene="priB"
                     /locus_tag="ACHURJ_002097"
     CDS             complement(1538..1852)
                     /gene="priB"
                     /locus_tag="ACHURJ_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFR1281041.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            complement(1859..2254)
                     /gene="rpsF"
                     /locus_tag="ACHURJ_002098"
     CDS             complement(1859..2254)
                     /gene="rpsF"
                     /locus_tag="ACHURJ_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFR1281042.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            2581..2856
                     /gene="yjfY"
                     /locus_tag="ACHURJ_002099"
     CDS             2581..2856
                     /gene="yjfY"
                     /locus_tag="ACHURJ_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFR1281043.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            complement(2931..3482)
                     /locus_tag="ACHURJ_002100"
     CDS             complement(2931..3482)
                     /locus_tag="ACHURJ_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HFR1281044.1"
                     /translation="MAINGLFVRTDPQRRRYGVALLVGVLSGIISAFVKWGAEVPLPP
                     RTFSGGRDEFNPPYIFLRDYLGIDPTQTVYTFSEHIINPVMVTHIIFSLVFAIGYCIV
                     AEIFPKVKLWQGILAGLIVTVAVHGIFCPALNLTPPLTQLPFDEYASEILGHIFWFWI
                     IEIMRRDLRNRITHEPDPEVAIR"
     gene            complement(3579..4265)
                     /gene="ulaF"
                     /locus_tag="ACHURJ_002101"
     CDS             complement(3579..4265)
                     /gene="ulaF"
                     /locus_tag="ACHURJ_002101"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFR1281045.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGKYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(4265..5119)
                     /gene="ulaE"
                     /locus_tag="ACHURJ_002102"
     CDS             complement(4265..5119)
                     /gene="ulaE"
                     /locus_tag="ACHURJ_002102"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFR1281046.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARDWVKARMA
                     KAGMVEAA"
     gene            complement(5129..5779)
                     /gene="ulaD"
                     /locus_tag="ACHURJ_002103"
     CDS             complement(5129..5779)
                     /gene="ulaD"
                     /locus_tag="ACHURJ_002103"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFR1281047.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(5793..6257)
                     /gene="ulaC"
                     /locus_tag="ACHURJ_002104"
     CDS             complement(5793..6257)
                     /gene="ulaC"
                     /locus_tag="ACHURJ_002104"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFR1281048.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(6267..6572)
                     /gene="ulaB"
                     /locus_tag="ACHURJ_002105"
     CDS             complement(6267..6572)
                     /gene="ulaB"
                     /locus_tag="ACHURJ_002105"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFR1281049.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            complement(6588..8036)
                     /gene="ulaA"
                     /locus_tag="ACHURJ_002106"
     CDS             complement(6588..8036)
                     /gene="ulaA"
                     /locus_tag="ACHURJ_002106"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFR1281050.1"
                     /translation="MSAQHSGCAEYPVTSSSGDRLYNIFTVFFNQVMTNAPLLLGIVT
                     CLGYILLRKSVSVIIKGTIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISD
                     TYASMMATIDRMGDAYSWVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGL
                     IAVTLFIFGYSMWTTIICTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASW
                     IAYKVAPFLGKKEESVEDLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAM
                     AGKVNWTVYILQTGFSFAVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAA
                     IYSFAPNAVVWGFMWGTIGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFG
                     GWRAALKICLVMGMIEIFGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMA
                     VIIVIALAYMFFAGRALRAEEDAEKQLAEQSA"
     gene            8340..9404
                     /gene="ulaG"
                     /locus_tag="ACHURJ_002107"
     CDS             8340..9404
                     /gene="ulaG"
                     /locus_tag="ACHURJ_002107"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFR1281051.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTPPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQAAPQEIRVQWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            9512..10267
                     /gene="ulaR"
                     /locus_tag="ACHURJ_002108"
     CDS             9512..10267
                     /gene="ulaR"
                     /locus_tag="ACHURJ_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFR1281052.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(10264..11013)
                     /gene="yjfP"
                     /locus_tag="ACHURJ_002109"
     CDS             complement(10264..11013)
                     /gene="yjfP"
                     /locus_tag="ACHURJ_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR1281053.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSSDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            11195..11524
                     /gene="bsmA"
                     /locus_tag="ACHURJ_002110"
     CDS             11195..11524
                     /gene="bsmA"
                     /locus_tag="ACHURJ_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFR1281054.1"
                     /translation="MVSRKRNSVIYRFACLLLVLMLSACSALQGTPQPAPPVTNHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            11673..11948
                     /gene="yjfN"
                     /locus_tag="ACHURJ_002111"
     CDS             11673..11948
                     /gene="yjfN"
                     /locus_tag="ACHURJ_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFR1281055.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(12065..13690)
                     /locus_tag="ACHURJ_002112"
     CDS             complement(12065..13690)
                     /locus_tag="ACHURJ_002112"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFR1281056.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSLLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMCHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(13774..14832)
                     /locus_tag="ACHURJ_002113"
     CDS             complement(13774..14832)
                     /locus_tag="ACHURJ_002113"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFR1281057.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNAIQERLISRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQ
                     QAGQESRFIYIEDLGLGVGGVLTDLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQW
                     VEPLWKSILSNKGLMPLLWRFFPGHPNLLASWFEGEKPQIAAGESYVRKPIYSREGGN
                     VTIFDGQNNVVDHADGDYADEPMIYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIRED
                     NTLITKDTSRFVPHYIAG"
     gene            complement(14835..15473)
                     /locus_tag="ACHURJ_002114"
     CDS             complement(14835..15473)
                     /locus_tag="ACHURJ_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFR1281058.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWLNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(15483..15881)
                     /locus_tag="ACHURJ_002115"
     CDS             complement(15483..15881)
                     /locus_tag="ACHURJ_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFR1281059.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAVSLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(15899..16558)
                     /locus_tag="ACHURJ_002116"
     CDS             complement(15899..16558)
                     /locus_tag="ACHURJ_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HFR1281060.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSRIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKTMGAMTLNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(16609..17307)
                     /locus_tag="ACHURJ_002117"
     CDS             complement(16609..17307)
                     /locus_tag="ACHURJ_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HFR1281061.1"
                     /translation="MGTLKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSR
                     AQDVLARLQRQQGE"
     gene            complement(17380..>17503)
                     /locus_tag="ACHURJ_002118"
     CDS             complement(17380..>17503)
                     /locus_tag="ACHURJ_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HFR1281062.1"
                     /translation="QKMMPLSSVGISSVQQEEYYIVFGALSLKSSLEDILLEIT"
BASE COUNT         4463 a         4677 c         4189 g         4174 t
ORIGIN      
        1 tcgtccaccg acaaaccagc aaaattaaaa tttcatcaat acgcacgcgc tacattactg
       61 tcaaatcgtg gaagagatca taattgcgat ggcagacgta ctgttgagat ccacaaacta
      121 gatctctctg actggcaagc attcaataag ttagcaacga agtgtaatgc ttacgatgga
      181 gtaacaatga atggtgataa ttcttttgga tggaaccatg aagctacatt agataatatt
      241 cacgcccaga aatacaataa agcttacgcg ggtgcgaggt taaccgcaga actgaaatat
      301 ttattgcaag atgtcgaatc cttcgaaccc aacagtaaat acacaataca cgaagtggtt
      361 ttgggacctg gttatggtac tccagactat accggccaaa cgataggata cgttgtaacc
      421 cttcctgccc aaatgcctaa ttgctggtca tctgaactac caacaatcga tctttatatc
      481 gaccaacttc acactgttac aggcgtttct aatgccctcg gattcatcat tgctgcattg
      541 ctaaacgcgt attctgattt accccatgat ttgaaaattg gtctgcgttc cttatcaaat
      601 tcagcagcaa tctactcggg tttaggtttc gagcgagtgc cgcaggaccg agacataagg
      661 agtgatcgta tgcatctaac gccagcaaac catcctgact tatggacaca agagaatggt
      721 gaatggatct acttgcgaaa ctaatacctg acgaaaaata gaaagcaaaa cgccgaccaa
      781 tggtcggcgt ttttacgtct cgttgaataa cgaattattc agctactacg tttacgatca
      841 ctttcgcgaa tacttcgctg tgaacctgga agctcacttc gtgttcgcca gtggtacgca
      901 gaacgccgtt cggcagacga acttcgctct tagccacttc aacgccagct gcagttacag
      961 cgtcagcgat gtcgcgagta ccgatggaac cgaacagttt accttcgtcg ccagctttag
     1021 acgcgatggt aacagtttcc agtgcattga ttttctcagc gcgagcatta gcagctgcca
     1081 gaacttcagc cagtttagct tccagttcag cgcgacgtgc ttcgaagaat tcaatgtttt
     1141 tcttggtagc tggaacagct ttaccctgcg gtaccaggaa gttacgagca tagcccgctt
     1201 taacgtttac ctgatcacct aggctaccca ggtttgctac tttatcaagc agaataactt
     1261 gcattacctt atcctctcaa agtcgtatta atggaccgtg accgattact gatggcgatc
     1321 agtgtacggc agcagggaca ggtagcgagc gcgtttgata gcgcgagcca gctgacgctg
     1381 gtattttgca cgggtaccgg tgatacggct tgggacaatc ttaccgcttt cggtgatgta
     1441 gtttttcagc gtagcgatat ctttatagtc gatctcttga acgccttccg cggtgaaacg
     1501 gcagaacttg cgacgacgga aataacgtgc catatggcta gtctccagaa tctatcaatt
     1561 caatctgctc ggcatgcaaa accattttgc tcagtccgtt ctttgccttg tggcatgaaa
     1621 tgaacccctg aacggttatg cgactgccga ccgttatact gtgagtaatg gcttggtttt
     1681 cgtgtccgct aacaataacg ggcatttggc accacgcctg ccggtgaaaa ccggcttcct
     1741 cctgcacaga acgatgctca agcacgaact ggcagtgagg aattcctgat ggactgacct
     1801 ttcgaagggg agtcctgcac acggtgccgg acaacaccag acggttggtc atcagaaatt
     1861 actcttcaga atccccagct tcagcatcat cagcggtttc gtttgcgaaa tcatcgcgac
     1921 gctcacggcg ctcgtctttc gctttaacca tcggagatgc ttcggtaaca gcgtgcttgg
     1981 tacgcataac catgctgcgg ataacggcat cgttgaagcg gaaggtagtt tccagctcat
     2041 cgatcacttc ctgcggagct tcaacgttca tcagaacgta gtgtgctttg tgcagtttgt
     2101 tgatcgggta agccagctga cggcggcccc agtcttccag acggtggatc ttgccttctg
     2161 caccagtgat ggcagcagtg tagcgctcga tcatgcccgg aacctgttcg ctctggtcag
     2221 gatggaccat aaaaacgatt tcgtaatgac gcatcgaatt gctccttacg gattattcag
     2281 cctcctgtct gggtcagccg aatcccgggg aggcaaggaa cgtgttaaag gtcggctgaa
     2341 aaatgacgcg ttatagtact tgtatgcccc actaaactca aggccagccg acaaataaat
     2401 ctcatctttc gctcaaaaga actaactcgc tgatttgaag ttcatcacat tccgtcacag
     2461 tgttttttcg aacaactgca tcagaaatgg tcaccatcaa cttctttatc attgattaaa
     2521 attaaatcag aagatcatca cagaggagag cacacaaagt cagccgtcga ggaggtatct
     2581 atgttcagtc gtgttttagc ccttctggct gtgcttttgc taagtgcaaa tacatgggca
     2641 gccattgaaa ttaataacca ccaggccaga aatatggacg acgtacagag cttaggcgtg
     2701 atttatatca atcataattt cgccactgaa agcgaagcac gtcaggcatt aaatgaagag
     2761 acagatgcgc agggcgcaac gtactaccac gtaattctga tgcgggaacc ggggagtaac
     2821 ggcaatatgc acgccagcgc ggatatttat cgctagcacc aggtataacc aacgaaacat
     2881 tgccatagtt tgctttgccc ccttcgcagg gggctttttt atacggttat tcaccggatt
     2941 gcgacttccg gatctggctc atgagtaatg cgattacgta agtcgcggcg cataatttct
     3001 attatccaga accagaaaat atgccccagg atttcagagg catattcgtc aaaaggcaat
     3061 tgagtgagcg gcggcgttaa attcagcgcc gggcaaaata taccgtggac tgccactgtc
     3121 acaataagcc ctgctaatat cccctgccat aatttcactt tcgggaaaat ttccgcgaca
     3181 atacaatacc ctatcgcaaa aaccagtgag aagatgatat gcgtcaccat caccgggttg
     3241 ataatatgtt ccgagaatgt atacacagtt tgggttgggt cgatacctaa ataatcccgt
     3301 agaaagatat acggcgggtt aaattcatcg cgaccaccgg aaaatgtgcg cggtggcaag
     3361 gggacttccg ctccccattt aacaaatgct gaaataattc ctgaaagtac acccacaagt
     3421 aacgcaacgc catagcggcg acgttgcgga tccgttctga caaacaatcc attgatagcc
     3481 ataaaaacac ctgttgttta tacgattttt taactgaagc ggccccggaa tcagaccaat
     3541 tacatcccgc aggaggtaat aattccgggg cgcactgctt acttctgccc gtaataggcg
     3601 ttaggaccgt gcttacgcat gaagtgttta ttcatgagga agctgtcgat gtgattcagt
     3661 tgtgggttaa tactgcgcgc aatccacgcc attttcgcca cttcttccat caccaccgcg
     3721 ttatgcaccg cgtcgtgggc atctttcccc caggcgaacg gcccgtgctg atacaccaca
     3781 attcccggcg tatgcagcgg ctcggcgtca cccagcgttt cgataatcac tttgccggtg
     3841 ttcagttcat actcgccctg cacctcttct tcgcttaatc cacgcgtaca cgggatgtcg
     3901 ccaaagaagt agtcggcgtg cgtggtgcct aacgccggga tcgccagccc cgcctgcgcc
     3961 catgcggtgg catgagtgga atgggtatgg acaatgccgc cgagcgacgg gtaacgacgg
     4021 tagagttcaa gatgcgtcgc ggtgtcagaa gacgggcgat acttcccttc taccaccttg
     4081 ccgctcatat cgaccaccac catatcgtct gctttcatgg tttcgtaggc aacgccgctg
     4141 ggcttgatca ccaccagccc gcgttcgcgg tcgatagcgc tgacgttgcc ccaggtaaag
     4201 gtcaccagcc catagcgcgg cagatccatg ttggcttcaa acacctgctg ttttagcttt
     4261 tgcattatgc cgcctccacc atgcctgctt tggccatgcg cgctttcacc caatcacgcg
     4321 ctttcaccac ttctgccgcc gggtcttccg ccgtttcgct ccacatctca atcaggtacg
     4381 gcccgcaata gccactctgt ttgagcgttt cgaagcaacg ttcgaaatcc accacacctt
     4441 cgccaaatgg cacgtttttg aagacgcccg gtttggtgtc tttcacatgc accgcgacga
     4501 tatgcccgat tccggcctgc aactccatct gcacatcgtt gtcccacgcc gacaggttgc
     4561 cgatatctgg gtagagctgg aaccacgggt tgttgagata gtgcgcgtaa cccagcgcct
     4621 tgctgatgga gttcatcaac ggataatcca tgatctccat cgccagcgtc acctgcgcgc
     4681 ggctcgccat ctcaacgctc tctttcaggc cgtcacggaa acgacgacgc gtttcgttat
     4741 tggcttcctg atagtaaacg tcatagcccg ccagctggat cacgcgaatg ccaacgtcct
     4801 gggcgaactg gatggcttta cgcataatct ccagcccctg cgcccgcacc gcgtcatctt
     4861 cgctgcccaa cgggaaacga cgatgggcag aaaggcacat ggacggcaca cgcacgccgg
     4921 tttcaacaat cgcattcacc agcgccagac gctgctcgcg gctccagtcg aggcgcgaca
     4981 ggcgctcgtc agtttcatct accgacatct cgacaaaatc gaagcccaac gtttttgcca
     5041 gtcgcaggcg ttccagccag cactccccgg cggggagcgc tttttcatag atgccaagcg
     5101 ggatttgttt ggacaacata tccgctcctt agccccacag ttcggcgatg gaacgtttga
     5161 actgacgcgc ggcttcaacc ggagaagcgg catcacggat gctgcgaccg gcgataaaca
     5221 catgaattgg aatgcctttg aacagcggca gatcttccag cgccaggcca ccggtgacgg
     5281 tgactttgaa gcccatatcg gaaagacgtt tgatcgcagt gatatcggct tcaccccacg
     5341 ccacgccagc ggcctgcgcg tcacggctgc ggtgataaac cacctgctga atacctgcgt
     5401 cgcgccactg ttgcgcctgt tcccaggtcc agtaaccggt cagttcgatc tgcacgtcgc
     5461 cgttaaactc ttttgctacg tccagcgcac ctttggcggt gttgatatcc gcacaacaaa
     5521 tcacggtcac ccagtcagcg ttggcttcga agcacatacg agaaagaatt ttgcccgcat
     5581 cggcaatttt ggcgtctgcc agcacgattt tgtgcgggta gagcgctttc agatcgcgaa
     5641 ccgcacgcac gccttcgccc acgcacagaa tggtacccac ttcgataatg tcgacttctt
     5701 cggcaatcag gcgagtggtt tcgtaggcgc tatccatagt ctggttgtcc agcgcgactt
     5761 gcaacatcgg taatgacatc ttcaattcct tcttaagctg ccgcgttggt gcggtcaatg
     5821 agatccagta cttcctgctc ggtacggcag gcacgtaaac ggtcgaaatt ttcttcatct
     5881 tcaaacaggt tgacgatctg catgatgccc acttcctggt gcgtgttggc atcgaccgcc
     5941 gccatggtga tgaggatatc caccgggtcg ttatcttcgt ggttgaactc cagcggcttt
     6001 ttcagcgtca ccagcgcaaa accggttttc ttaacgccct cttccgggcg accgtgcggc
     6061 attgccaggc ctggtgcgag cacaaagtac ggaccgtgct gttcaaccgc atccagaatc
     6121 gcctggtagt aacgcggctc gacaacatcc gccgccacca gcaggtcaac gccgattttt
     6181 actgcgtcct gccatgtctc agcttcagcc tgcaggcgga tggatttatt ctccgccagc
     6241 gaatcacgta atttcatggc gcgtccttac ttcacatcct gcgggaaatg cgctttgatc
     6301 acttccagca gtttcgggcc aaagtcggca ggagagagca tgttgcgcac gccaaccacg
     6361 tatttgttgc cggtcacggt gatttcgccc gcaatgtgcg tagaagcgat gatgatatcc
     6421 gcgccgctca actcgctttt gtattcgcca accgcgcagc tatttaccgt gtggtcaatg
     6481 tttgattggg ttaaaaactg gtccactttc atcttcatga tcatggaact gccttgtccg
     6541 ttgccacaca cagccagaat acgtacggtc ataatcaaaa ctccttatta agcagactgt
     6601 tctgccagtt gtttttctgc atcttcttct gcgcgcagtg cgcggccagc gaagaacata
     6661 taagccagtg caattacaat gatgacggcc ataaaggcga taccgatgga gaagaagccc
     6721 tgcatcatcg gcggtgccag aatcgaccag tccgccatgc ccatccaggc gctcataccg
     6781 gtgagtttca ccgcccagac gcagccaaag atttcaatca tccccatcac cagacatatc
     6841 ttcagcgccg cacgccagcc gccgaagtgg ttagcgaaca cgccgatggt ggcgttggag
     6901 aagaacatcg ggataaagcc gggaataatc aggatcgagg aaccgcaggc gaccaggatg
     6961 ccaaccgcaa tcagctgacc gatggtgccc cacataaagc cccaaaccac ggcgttcggc
     7021 gcgaagctat agatagccgc acagtcaatc gccagaaccg caccagggat caggcgctgg
     7081 gagataccgt taaatgcttc agagagttcc gccacaaaca tgcgcacgcc ctgagtgatg
     7141 atgaagatcg ccaccgcgaa ggagaaacca gtttgcagga tgtacaccgt ccagttcact
     7201 ttgcctgcca tcgcctgcac ggtgtcgata ccgaaggaga gtagaatggc accaaagaag
     7261 atggtcatca caatcgccgt ggagacgatg ttgtcgtgga aaatattcag ccagcctggc
     7321 agtttgaggt cttcaacgct ttcctctttt ttgccgagga acggcgcaac tttataggca
     7381 atccatgatg caaactgttg ctggtgaccg atggagaaac cgcagccgtc cgtcacttcc
     7441 tgagtcggct tgtacatcat attggaggta atgccccagt agagcgaaac cagaatcgcc
     7501 gtgcagataa tggttgtcca catggagtag ccgaaaataa acaacgtaac ggcaatcagc
     7561 cccgcctgct ggaacatgat atgaccagtc agcatgatgg tacgaatacc ggtaatgcga
     7621 cgcagcagca cgtaacagat gttcagcgcc agcgctaaca acaccgcgta acctacccag
     7681 ctataggcat cgcccatgcg gtcgatggtt gccatcatcg acgcgtaagt atcggaaatt
     7741 gcgccgttaa tgccgtagac ttcggacatt ttcgccacca ccggtttgaa ggtgctggtg
     7801 aggatgccgg accctgcctg caacaacatg aaaccaatta tggttttaat cgtgccttta
     7861 ataataacgc tgacactttt gcgcagtagg atgtagccca gacaggtcac aatacccagc
     7921 aacaacgggg cattggtcat gacctggtta aaaaacacgg taaagatgtt gtagagacga
     7981 tctcctgaag aagaggtaac cgggtattcc gcacacccgg aatgttgtgc actcactcta
     8041 attttcaaaa gcaatcacaa caagattatt tatgattaaa tgtgacgcga cccgcaaata
     8101 attccatacg gatagagtgg gattcacaca acgaaacaat tatttattta aatttagtta
     8161 aattcattta gttaatttaa ctatacattc cattaaatct ttccacactc aaaatcaaca
     8221 acaacttccc tggcaaaaag tcaaaaaatg gtcattgcct tcctgaaaaa atagtgtcag
     8281 gattaatcaa aaccaatcac atattgattc gaattgaact atcatgaagg taaatggcga
     8341 tgagtaaagt gaaaagtatc acccgagaat cctggatcct gagcactttc ccggagtggg
     8401 gtagctggct gaatgaagaa attgaacaag agcaggtcgc ccctggcaca tttgcgatgt
     8461 ggtggctagg ctgcaccggg atctggttga aatcagaagg tggcaccaac gtttgcgttg
     8521 atttctggtg cggcactggc aaacaaagtc acggtaaccc gttaatgaaa cagggtcacc
     8581 agatgcagcg catggctggc gtgaaaaaac tgcagccaaa cctacgcacc cccccgtttg
     8641 ttcttgatcc gtttgcgatt cgccagatcg acgcggtact ggcgactcac gatcacaacg
     8701 atcatatcga cgttaacgtc gctgctgccg tgatgcagaa ctgtgctgat gacgtaccat
     8761 tcattggacc gaaaacctgc gttgatttgt ggattggctg gggcgtaccg aaagagcgtt
     8821 gcatcgtggt caaaccgggc gatgtggtga aagtgaaaga cattgaaatt catgcgcttg
     8881 atgctttcga ccgtactgca ctgatcaccc tgcctgccga tcaaaaagcg gctggcgtac
     8941 tgccggacgg catggacgat cgcgcggtga actatctgtt caaaacgcct ggcggctccc
     9001 tgtatcacag cggcgactcc cactactcta actattatgc gaagcacggt aacgaacatc
     9061 agatcgacgt ggctttaggt tcatacggcg aaaacccgcg cggtatcacc gacaaaatga
     9121 ccagcgccga tatgctgcgt atgggtgaag ccctgaatgc gaaagtggtt atcccgttcc
     9181 accacgatat ctggtcaaac ttccaggccg ctccgcaaga gatccgcgtg cagtgggaga
     9241 tgaaaaaaga tcgtctgaag tatggcttca agccgtttat ctggcaggtt ggcggcaaat
     9301 ttacctggcc actggataaa gacaacttcg agtaccacta tccgcgcggt ttcgatgatt
     9361 gcttcactat tgaaccggat ctgccgttca aatcattcct gtaatctgtc gtttataccg
     9421 gatagcgtgg agaagttcgc gctatccggc gtagtatttc ctggctattt caaatatcat
     9481 ctaaaaaaat caaattttat cggaatagct catgactgaa gcacaaagac atcaaatcct
     9541 cctggaaatg ctcgcacaat tgggctttgt gaccgttgag aaagtcgttg agcgtctggg
     9601 aatttcgcct gccactgcgc gacgcgatat caataaactt gacgaaagcg gcaaactcaa
     9661 aaaggtgcgc aatggcgcag aagctattac ccaacagcgc ccgcgctgga cgccgatgaa
     9721 tctgcatcag gcgcagaatc acgatgaaaa agtacgtatt gctaaagcgg cctcgcagct
     9781 ggttaatccg ggcgaaagcg tagtcatcaa ctgcggctcc accgcgtttc tgcttgggcg
     9841 ggaaatgtgt ggcaagccag tgcaaatcat cactaattat ctaccgctgg caaattacct
     9901 gatcgatcaa gaacatgaca gcgtgatcat tatgggtgga cagtacaaca aaagtcagtc
     9961 catcacttta agcccgcagg gcagcgaaaa cagtctctat gccgggcact ggatgtttac
    10021 cagcggaaaa gggctgaccg cagaagggtt gtataaaacc gatatgctga cagcaatggc
    10081 agagcagaag atgctgagcg tggtagggaa actggtggta ctggttgata gcagtaagat
    10141 tggcgaacgc gcgggaatgc tttttagccg tgccgatcaa atcgatatgc ttatcaccgg
    10201 caaaaatgct aacccggaaa tcctgcaaca actggaagcg caaggggtca gcattctgcg
    10261 tgtttaaaga tgctggcgga aaaatgtcac ggcagcatct aacgcctcag gcgtaatgcg
    10321 gtgacgcacg cctggctgcc atgaacaggt tagcagttta tcccgtcccg tctcgcttaa
    10381 ggcctgctgc aaacgtagtg attcgtcggc aggcacaacg tcatcgtcga ggccatgcca
    10441 cagcagtaga ggtctgtccc caagttgttc caggtggttt gtcgcttccc actctgccag
    10501 cggcgcgata atgttattga attcattctg ctgtgctgcc gtttcaggta tcagcggtgg
    10561 aaacagtgaa cgggcgagcg atgtaaaata gcccgatccc atcatgctgg cggtacattt
    10621 caccgtgggg tggcgagcgg taattcccag tgccgtcatc gcgcccatcg acgcgccacc
    10681 gactgccaga cggtcatcaa gcagccagtt ttcttcggct attgccgcac gtaaagtagt
    10741 gaattcctgc atactttgta gcaagatttg ccagaattga tttaaccgcc gcgcttcgtc
    10801 gctactaaaa cggctaccgt gatcgggcgc atccggcatg atcacccgca aaccagcctg
    10861 cgccagcgca acggcaaaat agctatacac caggctggat gaagtaaagc cgtgataaaa
    10921 aattacgcac ggtaacgggg tatctttttg cccgacagga taagcatgaa gaacgggaat
    10981 atctgccagc tcgcgtgatt ctatttcaat catctgtctc tcctttttgt ctggtatgac
    11041 atgaagaaat ccttagcaca agtttctgct ggcggtatta gttcataatg ttgagatgtg
    11101 ggttacgctt tcgtcacgtt ttcattcgaa aatcgcgtac caggtaacaa ttcgggaaca
    11161 ttcccccaaa gttaaattaa taaggcacta cactatggtt agcaggaaac gtaatagcgt
    11221 tatttaccgg tttgcctgtt tattattggt gttgatgtta agtgcctgta gcgcactgca
    11281 aggtacgcca cagccagcac cgccagtaac gaaccatccg caagagattc gccgcgacca
    11341 gacgcaaggg ttacagcgaa taggtagcgt aagcacgatg gttcggggtt ctccggatga
    11401 cgcattagca gaaatcagag caaaagctgt cgctgcaaaa gctgattatt acgttgtcgt
    11461 catggtggac gaaaccatcg tgacaggaca gtggtattca caagccattt tgtaccgtaa
    11521 ataaatcaga caacttacat agtcagcttt acattgcttt gcgtccctat gcgtttacct
    11581 gtgcacaata aattaccggc tggaagcctg ggcgtttatt accgcgatgg aatgccctgc
    11641 aacatgtggg gaaacgaaaa atggagctga cgatgaaaca attacttgct tcaccctcgc
    11701 tgcaattagt gacttatcct gcgagcgcca cggcacagtc tgcagaattc gccagtgctg
    11761 actgcgtgac gggcctgaat gaaataggtc agatctcagt tagcaatatt tcaggggatc
    11821 cgcaggacgt ggaacgtatc gtggcgttaa aggccgatga acaaggtgcg tcatggtacc
    11881 gcattattac catgtacgaa gaccagcagc ctgataactg gcgcgtacag gcgatactgt
    11941 atgcatgagt gtcatctaca gcaaatgaac aatgccggat tcggcgcgag cgccttgtcc
    12001 ggcctacaat tttcacatat ttcattgagt taatcgaaac cagcgtcgca tcagtcgtat
    12061 acgcttacac acacactccc cccgtcgccc gcagcaataa atcattctgg atctgctctg
    12121 acaaccgtac gccgccgcgc gtatctaaca tcacctgaca ccacgcctgc gccattggcg
    12181 gagaagcata tttcaacatt tgcgcaccgc agcccagcag gaatagctga tgagtaatct
    12241 ctcgccccag ttcttcagct ggcttacgca gctgctgctg taaacgacga accgcgcgat
    12301 caaaatagcg atcctgccct ttcacttcca caaatgcttc cgacaataag tcgtatacgc
    12361 ccgcttgctt attgagaacg cgcaacacat ccaggcacat aatattgccg gaaccttccc
    12421 aaatactgtt taccggcatc tcccggtaaa gccgcggcag ctcgctctcc tcgcaataac
    12481 caatgccgcc cagcacctcc atcgcttcgg ccacaaacgg catacctctt ttgcagatca
    12541 caaattttgc cgcaggcgta aataaacgcg cccacagggc ttctttggca tcggcacgcc
    12601 ggtcccacgc tcgcgcaaga cgaaacagca acgccgtttg cccttcaagc tgaagtgcca
    12661 tgcgacttaa gacatgacac ataaggggct gttggatcaa tggattacca aaaacatggc
    12721 gttgatgtgc atgataaatc gccagcgaaa atgcacggcg catcatggca tggctaccca
    12781 gggcgcaatc aaaacgcgtc atcccaccca ttttcaggat cagacgaatt ccttcccctt
    12841 cctgccccaa caaccagcca atggcatcct gaaactccac ttcgcaactg gcgttagagc
    12901 gattacccag cttatctttc agccgctcga ggcgaatcgc gttgcgttgc ccatcaggca
    12961 aaaagcgcgg cacaaaaaag caggacaaac cgcccgtagt ctgcgccagc accagatgcg
    13021 catcgctttg cggcaccgag aaaaaccatt tatgccccac cagccgataa gagccatctt
    13081 ccagacgctc tgcacgggtg gtgttgctca tgacatcgga accgccctgc ttttccgtca
    13141 ttcccatgcc aatcaacaaa ccgcgttttt gcccacctgg caataagaga gaatcgtagc
    13201 gatcgctcag cagcggcgtg gtccagtcct gaaacggcgt gggtaacatc tgtaacaaca
    13261 atggcgtggc ggcaaaggtc atcgttattg gacataacga cccagcctca acctgcgcat
    13321 gtaacataaa acgcgccgcg cgcgccacaa atgcgccgga gcgagcgtct tcttcccagg
    13381 caagattgtg cacccgattg gtacataacg cctgcatcag caggtgccag gcggggtgaa
    13441 aacgcacatc gtccagacgg cgtccttgcg catcgtagcg caataattca ggcggattca
    13501 cattcgccag ccgccccagt tcaagagatt cagccgttcc taactgctga ccaatactgg
    13561 ctagaaaatc gctatcccag ccagcacctt cacgcgttac cgcttcacag agcgcgccat
    13621 cagacaggta taagttgctg ttatttaatg gtataggttg attaaaaacg gtgtgagttt
    13681 gccagtgcac tgtgtctccc tccatcaatg gcagtcaccc ttagtatggt cactgccatg
    13741 gattcctgac agaaagagat tgctaaaaca ttcttatcca gcaatgtaat gcggaacgaa
    13801 acgtgaggtg tctttggtga tcagcgtgtt atcttcacgg atccccattc cgcacgcttc
    13861 atcatcgaca atccagctac cgatgagtgt gtagctatcg ccaaatcgcg gcagaggttg
    13921 aaacgcctgg tagatcatcg gttcatcggc gtaatcacca tcagcgtggt caacgacgtt
    13981 attctgaccg tcgaaaatgg tgacgtttcc gccttcgcgt gagtagatcg gtttacgcac
    14041 atagctttcg ccagcggcaa tctgcggttt ttcgccctca aaccaggatg caagaaggtt
    14101 aggatgacca ggaaagaagc gccaaagcag cggcattagc cctttattac tcaagatact
    14161 tttccataac ggctccaccc attgctcgcg acgcttgcgc agcagcggac cgttatcgtc
    14221 acgcatcatc cactccagcg gatacagctt aaatgcacgc tggatgacat tatcatcaag
    14281 atcggtcagt acgccgccga cgcccaaacc gagatcttca atgtagataa accgcgactc
    14341 ctgccctgcc tgctgggcgc agtcctgcaa gtacagcacg gtactcctgt cttcatcggt
    14401 gtcctgacag cagcaaaaat aaaacggttc ccgactgtaa agctcactaa agcgcgaaat
    14461 caggcgttcc tgaatggcgt tgtactccag cagcttcacc ggcgcattgc cacaccaggc
    14521 aaaatccatc cggccataca gcgaaggatc gcgtgcgcgc cagctttcgg cgatcacatc
    14581 ccagtacaac ggcgggatcg ccagttgcgt caggatctct tcgtctttaa ccgcgcgatc
    14641 caccacctca aggcacatct gatgcagttc cgcagtcggt ttttcgatct gctcttcaat
    14701 ctggcgcaga gtaaaacggt aagcccgact ctcgtcccaa tagatttcat tgtcgatgat
    14761 atgaaagtcg aaaccgttgt tagcggcgat ctggtccaga tcccgtcgca caggaacgtt
    14821 gtgtctcagc atgattagcc tccccaatgc ccacgggcgc tggaagaacg accatagccg
    14881 ccgcgcgaaa cggttgacgc ttttttggtg gtaaagccgc ctgaagagta agactctttt
    14941 ttgccagaac cggagcgcca ggagtaatca ccggaagtgt tgagccagac ggggcgggaa
    15001 gcaaatgagg atccgccgct gttatagacg aacggctcat cgcgatcttt acgaataacc
    15061 cggcttaaca aaaatccaga aacgaccggg atccaactct gttcaacatt gtcgtagtag
    15121 caattttcgt acttagactg acaattctgc tgagtcatat tcttcggaac atcggcataa
    15181 aatgccgctt tggcgttgtt ccagccacgc gcgcaaatgt cggcattatt accgtcatca
    15241 atacaatcct gcactgtcga ataaaacgtt ccgtcgccgt cgttatcgac atcgccacta
    15301 tcgccacaac ctttcaatac aaaaaaagcg gcaccgccca ttagcgccaa cgttaagtat
    15361 ttttgtgcgt agcgattgcg acgcggttca aaaggattat tcggtctacc aatgcgactg
    15421 atcgctccgt gaccaatttt actgttattt cttgatttgc gttttctggc catgatcgtc
    15481 cattaccatg tcatacaagc tgcgttaaaa ataccgccag ccagcgcggc ggttcccata
    15541 aacattcctg ctgcggtatt gtgattaata attttttcgc ttaatgcggg catataaagt
    15601 ctgacaccag caaaaacgag taactgaatc accagtgcaa tcccgcccca ggcgaaatag
    15661 tctggaatac tcaccgcatt gattgccgca ctggataagg ggatcacgta acccaacaat
    15721 gtaccgctga atgccagtga caccgcggta ttattgtttt tgattaattg ccattcgttg
    15781 tgcggtgtaa ttttggagta gataaacaga aaaataatca ccatagccac gccaataaaa
    15841 aaataggcac taaaggcaag aagtgaatcc agtatatgca ttgtaaacgt ccttgtaatt
    15901 aaccaataat atgaagtgat gtcagcggga tatcgacacc caacgcgcgc gaaaataacc
    15961 actctggctc gccgagatcg ttaaaagatt cctcaccatt taacagtaaa tattcgtaag
    16021 tatcttcggt tacttgccgc tggtagccca tggtaaaatt atggacctcc catttggcat
    16081 ggttttgatt ttctactttt tccagcatgt agacgggttc gatatttccc ggttcttcgc
    16141 tgttaaaaaa tcgctgccag cgtttttcct gccagtttaa ggtcattgcc cccatcgttt
    16201 tggcgttgat cgcctcgcgc cagtgactct ctttactgat accgtagctc tcttcataga
    16261 caaagagctt gatatcatca atgtcatcaa tatcttcgcc gccggtggta ttgatttgca
    16321 gaaattcatc gcctgaagtg tagtaacgga aaatctggct accgcctccc agatcgatgc
    16381 ggctgacagc ggctaccgta aattcctcac ccggtagcgc aatcagcaat tcatcttcca
    16441 gcaaacgaaa cgctaacgtg tcgagcgtaa agccactatt gagatgaagt cccagcggac
    16501 cgcgagcgat agcaggctta ttatccttgc caaacagacg ctggaaaaaa ccagacatat
    16561 tttatcctta aaatagttgc cagcctttta gcgaggctgg caaaaaaatt actcgccctg
    16621 ttggcgttgc agtcttgcta atacatcctg ggctctactt ttattgctac cgccaattcc
    16681 ggcttccgcc agcttttcgt caaggtcgcg accgtctgcg actttctcca actgtgcggc
    16741 agcatccagg cgagcctgac gttcggcctg acgcgtttgc aggcgtttta aggattctgc
    16801 cgctgtcgaa acgctggaag atgcgccaac ggtagaggtt gttacagcct gttgtgcacg
    16861 ctgcatggct tcagtggctt taacgacttc catttgctgc tcaaactgag cgatacgctg
    16921 cgctgtcgct gtaactgctt tttccacgcc atcacgggag acttccagat tcgacaacac
    16981 ttgctcttca gcggtaatga gattctcaag gcgcgcgatt tcttcagcaa cttcgttaat
    17041 caacgacgga ttaacgttct tgcttaacgc ttccagcgca cgggcttcca gactggcttt
    17101 gcgctcacgt aaatctttca gcttatcgtg acttaatttc acccgcgcca gcagatcaac
    17161 gcgagatttt ccggctttat cgagttcagc tttagcgtca cgaatatgct gttccagcat
    17221 gcgcacgccc tgggtttctt caatggattc ttccgcctgg gagataaacg atttccccag
    17281 cgtaaataaa ctttttaaag ttcccataaa tacccgtcct tgtaattagt gtgaatattc
    17341 ttctgtaatt tcagccagat ccaatgcgtt atctaccagc taggttatct caagcaggat
    17401 atcttcaaga gaagatttaa gcgataacgc cccgaaaaca atgtaatact cttcctgttg
    17461 cacgctggag attcctaccg atgacaacgg catcatcttc tga
//
LOCUS       DAZPEB010000072        17502 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZPEB010000072 DAZPEB010000000
VERSION     DAZPEB010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17502
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.073"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..321)
                     /locus_tag="ACHURJ_002119"
     CDS             complement(<1..321)
                     /locus_tag="ACHURJ_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BAX inhibitor (BI)-1/YccA family protein"
                     /protein_id="HFR1281063.1"
                     /translation="MESYSQNSNKLDFQHETRILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPTMYVAIGLLLVCMYGLSKDINKINAVIAGVIYLFLLSLVAIVVASLVP
                     VYAII"
     gene            complement(527..1231)
                     /locus_tag="ACHURJ_002120"
     CDS             complement(527..1231)
                     /locus_tag="ACHURJ_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFR1281064.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(1368..1820)
                     /gene="moaE"
                     /locus_tag="ACHURJ_002121"
     CDS             complement(1368..1820)
                     /gene="moaE"
                     /locus_tag="ACHURJ_002121"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFR1281065.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFAGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(1822..2067)
                     /gene="moaD"
                     /locus_tag="ACHURJ_002122"
     CDS             complement(1822..2067)
                     /gene="moaD"
                     /locus_tag="ACHURJ_002122"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFR1281066.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHMAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(2060..2545)
                     /gene="moaC"
                     /locus_tag="ACHURJ_002123"
     CDS             complement(2060..2545)
                     /gene="moaC"
                     /locus_tag="ACHURJ_002123"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFR1281067.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(2548..3060)
                     /gene="moaB"
                     /locus_tag="ACHURJ_002124"
     CDS             complement(2548..3060)
                     /gene="moaB"
                     /locus_tag="ACHURJ_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFR1281068.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(3082..4071)
                     /gene="moaA"
                     /locus_tag="ACHURJ_002125"
     CDS             complement(3082..4071)
                     /gene="moaA"
                     /locus_tag="ACHURJ_002125"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFR1281069.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(4059..4203)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            4468..5376
                     /gene="yvcK"
                     /locus_tag="ACHURJ_002126"
     CDS             4468..5376
                     /gene="yvcK"
                     /locus_tag="ACHURJ_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFR1281070.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(5568..7589)
                     /gene="uvrB"
                     /locus_tag="ACHURJ_002127"
     CDS             complement(5568..7589)
                     /gene="uvrB"
                     /locus_tag="ACHURJ_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFR1281071.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(8168..8845)
                     /gene="bioD"
                     /locus_tag="ACHURJ_002128"
     CDS             complement(8168..8845)
                     /gene="bioD"
                     /locus_tag="ACHURJ_002128"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFR1281072.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            complement(8838..9593)
                     /gene="bioC"
                     /locus_tag="ACHURJ_002129"
     CDS             complement(8838..9593)
                     /gene="bioC"
                     /locus_tag="ACHURJ_002129"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFR1281073.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRRSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(9580..10734)
                     /gene="bioF"
                     /locus_tag="ACHURJ_002130"
     CDS             complement(9580..10734)
                     /gene="bioF"
                     /locus_tag="ACHURJ_002130"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFR1281074.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFVHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYNTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(10731..11771)
                     /gene="bioB"
                     /locus_tag="ACHURJ_002131"
     CDS             complement(10731..11771)
                     /gene="bioB"
                     /locus_tag="ACHURJ_002131"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFR1281075.1"
                     /translation="MAHRPRWTLSQVAELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPNERDMPYLEQMVQGVKDLGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            11858..13147
                     /gene="bioA"
                     /locus_tag="ACHURJ_002132"
     CDS             11858..13147
                     /gene="bioA"
                     /locus_tag="ACHURJ_002132"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFR1281076.1"
                     /translation="MTKDDLAFDQRHIWHPYTSMTSPLPVYPVVSAEGCELILSDGRR
                     LIDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEVRQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHNLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            13206..13682
                     /locus_tag="ACHURJ_002133"
     CDS             13206..13682
                     /locus_tag="ACHURJ_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFR1281077.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVEHIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            13834..15117
                     /locus_tag="ACHURJ_002134"
     CDS             13834..15117
                     /locus_tag="ACHURJ_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFR1281078.1"
                     /translation="MNTFSVSRLALALAFSVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(15258..>17502)
                     /locus_tag="ACHURJ_002135"
     CDS             complement(15258..>17502)
                     /locus_tag="ACHURJ_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFR1281079.1"
                     /translation="KGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSSHNTSGN
                     MDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGTINGDDH
                     VFGLSAAQRYGGIFVPPHIAIIHQYMREMMAGGGKMILGSDSHTRYGALGTMAVGEGG
                     GELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVKNKVMEF
                     VGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQLNPQPMA
                     YYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAHGKAKLS
                     LLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPSSQPVFM
                     DLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREGSKPANG
                     QMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQGFVKGAT
                     QQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRSNPIGLA
                     EFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGS
                     MVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTF
                     EVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLIN
                     FNKNRQM"
BASE COUNT         4092 a         4734 c         4442 g         4234 t
ORIGIN      
        1 gataatggca tatacaggaa ctaaacttgc aacgacaatc gccaccaaag agagtaaaaa
       61 cagatatatt acgcctgcga tgacggcatt tatcttgttg atatccttgc ttaagccata
      121 catacaaact aataataacc ctatggcgac atacattgtt ggtgaatcat atttggttgc
      181 cgtaatttcg agatacttag ctccccaggc cagtcctgcg gttgccacca aacctaaagc
      241 ggtaatgagc catataccgt ttaatatcct ggtctcgtgc tgaaaatcta atttattgct
      301 gttttgtgag tatgattcca ttcttttagt tgtccttaga aaaaatatta atattcacca
      361 tcaataaatt taaacccacg catacaaaat acttttgtat ttatggatac ggaaaacgct
      421 tccgctcggc cataaggcgt attttatacc tggtacttgt taagtgtcgt ttttagtaag
      481 aaaagaaatc ccgacacatg gccgggatat tgatggtgaa aaagaattaa cggcggttgc
      541 caaaaattcg cagcaacatc aggaacaggt tgatgaagtc cagatacaag gttaacgcgc
      601 caagaatgga atatttgcgc aggttcgacg tgtcgcgggt atcaatctgc tcacccatat
      661 ttttcaattt ctgagtgtca tacgccgtca atccgacaaa gacaatcacg ccgatgtagg
      721 taactgccca catcaatgct tcgcttttca gccagaagtt gaccagcgat gccagcacaa
      781 tgccgattaa cgccataaac agcatattgc caaagccact taaatcgcgc ttcgtggtgt
      841 aaccgtacag gctcattgcg ccgaacatcc cggcagtaac gacgaaagta ctggcgatag
      901 aagcagcggt atagacaatg aatatactgg aaagcgtaag acccgtcagc gccgaataaa
      961 gcataaagag catcgtcgtt acacctgcgc tcagcttttg aatcatcgct gataacacaa
     1021 taaccaatgc taattgcgcg atgatcagac cgattaaaaa gacacggtta gtgaacaaca
     1081 gctccatcac ggccgcggaa ttagccgcat accaggcaac aaatgcggtc agcaacaagc
     1141 caacggtcat ccagccatag acttgcgcca tataagtttg caagccagcc cggggttgta
     1201 cgattgaatc agaacgtggg aatctgtcca tgacgatctc ctgaagatat aaggaatatc
     1261 ttaaggatac tgcaaaatga tgaggctgtg catcgacgca gcgtaaacgc atgtactgag
     1321 cggtgaaatt gccggacgca gcggtgcctt atccggctaa caaaaaacta ccagcgtttt
     1381 gccgcctgct gatcgctctc ccgagcttca acccagcggt cgccttccgg cgtggcttcg
     1441 cgcttccaga acggtgcgcg ggttttgaga taatccataa taaactgccc ggcttcaaac
     1501 gcactgctgc gatgcgcact ggtgacaccg gcaaaaacga tttcatcgcc cggccataat
     1561 tccccgatgc ggtgaatcac agtgacgcgc cccagcggcc agcggttacg cgcttcatca
     1621 acaatttctg ccagtgcttt ttcagtcatt cccggatagt gttcgagggt taatgctttg
     1681 acgctgtcgc ccaggttatg gttgcgcacc ttaccagtaa aggtgactac cgcaccgtct
     1741 tcgtcacgct ccgccagcca cgggtactct tctcctacgc tgaacggctg cggaccaaca
     1801 acaattttgg tttctgccat cttaacctcc ggttaccggc gggaagaaag ctacttcgtc
     1861 gccgtcagtc agcggatggt caaaactcac cagcgtctgg ttgacggcag ccagtaattt
     1921 gccatcttcc agcgccagcg cccagcgatc gctctgcgca gccatgtgct ggcgtaacgc
     1981 ttcaacagtt gggaaatccg cagccacttc ggttgcatct gttcccacca actcgcgcac
     2041 ctgcgcgaaa aaaagaactt taatcatccg cttccacctt aaagtcaccc gacttgccgc
     2101 cgctcttcgc cagcaaacgt accggaccaa tcaccatatc tttttgcacc gctttgcaca
     2161 tgtcataaat ggtcagcgcc gccacggagg ccgcggttaa tgcttccatt tcgacaccgg
     2221 ttttcccggt caggcggcat aaggtttcta tacgcacccg attgtgctcc ggctcggcct
     2281 gtaaattgac ttcaactttg ctgagcatca gcggatgaca gagcgggatc agatcccagg
     2341 tgcgttttgc cgcctgaata ccggcaatac gcgcagtggc aaatacgtcg cctttgtggt
     2401 ggcgaccatc aataatcatc gccagcgtct cgctgcgcat ggtgacaaag gcttccgccc
     2461 gcgcttcacg cacggtttcc gctttggcgg agacatccac catgtgcgct tcgccagcgg
     2521 cgttgatatg ggtcagttgc gacatactta tttcttcaaa tgtggatgga aattacacgg
     2581 acgcgtacgg gcatccagct gcggcgcgat gatattttcc catgcggtac ggcacgcttt
     2641 ggtcgaaccc ggcatggcga aaatcagcgt tttgttggcg acgcccgcaa ccgcacgaga
     2701 ttgcaacgtg gaagtgccaa tctcttcaaa cgacaacatc cggaacactt caccaaaacc
     2761 ttcaacttca cggtcgaaca acggcagcaa tgcttcggga gcctgatcac cttccgtcag
     2821 gccagtacca cccgtaatca ataccacttg tacatcgtcg ctggcgatcc acgcagatac
     2881 ctgagcgcga atagcgtagc ggttttcttt cacaatggct ttatcgacaa cgtgatggcc
     2941 cgcttcttgc gccgaatcgc gcagatagtg accggaggta tcgtcttctt caccgcgacg
     3001 attagaaacc gtaagaatag caatacgggt cgggataaat tcagtgctta cctgactcat
     3061 ctgatctctc cttttgacgt tttagccgcc aatgtacgat aagttttgcg taataccggt
     3121 gttgttttga tgcaggaaat gggtctgttt cttctcccgc agcgccgctg aaatacgcgc
     3181 ttccagcgcc tgttgctggg tatcgtcttc cagcagatcg cgcaggttaa cgccgccttc
     3241 accaaacagg cagagatgga gtttaccaat ggaggaaacg cgcaggcggt tgcaagtggc
     3301 gcagaagtct ttttcatacg gcatgataag gccaatctct ccggcgtaat ctggatggca
     3361 aaagacttgc gcgggaccgt cgctgcgttg acgtaattgg tggatccagc cgcgacgcag
     3421 tagctcgtca cgcagaacct gaccagagat gtgatgctta cggaagagct cgctgccctc
     3481 gcccgtttcc atcagttcga tgaaacgcag ctggataggg cgatgctgga tccagttcag
     3541 aaaggtgtcg agctggtgat gattaacatc acgcatcagc acggtattga ctttgacctt
     3601 ctcaaaaccg gcctcaaatg cagcatcaat ccctgccatg acctggttga atttatcctg
     3661 cccggtaata gcgtgaaact ggcgggcgtc cagactgtcg acactgacgt taatgccagt
     3721 aagtcccgca tcgcgccagt tcgccacatc gcgttccaga cggtaaccat tggtggtgac
     3781 cgcaatctgg cggatagcgt cgttttcccg cacagcggcg atgatatcgg taaagtcgcg
     3841 gcgtaaagac ggctcacctc ctgtcaggcg cactttttcg gtgcccagac tggcgaaggc
     3901 gcgcgtaacc cggcgaattt catcgacggt aagaaagcct ttattggtga cgccgctcgg
     3961 tttgtagcca tccggcaggc agtaggtgca acgaaagtta cacacatcgg taatcgacag
     4021 gcgcaagtag taaaacttac gcgcaaatgc atcagtcagt tgtgaagcca tgtacacctt
     4081 tccagatacg ggaggcgaag tcatttcttc cttcgccctg gtggcaatct ttacgagcat
     4141 tgtcacggcc aaagcaccgt atcagttgac ccaggtgcag aggctagagt gtttagtggt
     4201 taagccgata ctagcgtgta aatgagattt ttaccatcca cactttcgct atgcaatcat
     4261 gtatatagcg tcatgatcgt gctatttcgc tacagaataa gggaaaatgg cagttttact
     4321 ttgatataca tcatcaggga ggcatagcgt gcatcgtcct ttagaggtag aagaaaatag
     4381 tttgtatcct caatattggc aggttaattg ctgtttcccc gcaatttgcg ctactgtagc
     4441 gcggataatc tgactccagg aaactatatg cgcaatcgta cgctggctga tcttgatcgt
     4501 gtcgttgctc tcggcggagg gcatggactg ggacgcgttc tctcatcact ttcgtctttg
     4561 ggttctcgtt taacgggtat cgtcaccacc acggataatg gtggctcgac ggggcgtatt
     4621 cgccgttcag aaggcggcat tgcctggggc gatatgcgca actgcctcaa ccaactgata
     4681 acggaaccga gcgtcgcctc cgcgatgttt gaataccgtt ttggcggcaa tggcgaactt
     4741 tccgggcaca accttggaaa cttgatgtta aaggcgctgg atcaccttag cgtgcggcct
     4801 ctggaagcca tcaatttaat tcgtaatctg ctgaaagtgg atacgcattt gattccaatg
     4861 tcagagcatc ccgtcgatct gatggcgatt gacgatcagg ggcatgaagt ttacggcgag
     4921 gtcaatatcg accagttaac tacgccgatt caagagttat tattaacgcc gaatgtaccc
     4981 gcaacgcgtg aggcagttca cgctatcaat gaagcggatc tcatcattat tgggcctggc
     5041 agtttttata ccagcctgat gccaattctg ctgctgaagg aaatcgccca ggcattacgc
     5101 cgcacgccag cgccgatggt ttatatcggc aatctggggc gtgagttgag tttacctgcg
     5161 gctaatttga agctggaaag caagctggca attatggagc agtatgttgg taaaaaagtc
     5221 attgatgcgg tcatcgtcgg gccaaaagtg gatgtctcgg cggtgaaaga gcggattgtg
     5281 atccaggagg tactggaggc cagcgatatt ccgtatcgtc atgaccgcca gttgttacat
     5341 aacgcgctgg aaaaggcgtt acaggcttta ggttaacgga aatccgaagg aaaattccgg
     5401 cttcctattg aagacaaagt gcgcgttgtt tatgccggat gcggcgtgaa cgccttatcc
     5461 ggcctacaaa ccgcgcaaat tcaatatatt gcggagaaaa tgtaggcctg ataagcgtag
     5521 cgcatcaggc tgttttccgt ttgtcatcag tcttcttcgc tatcctgtta cgatgcggca
     5581 ataaacaact cacgcaactg atgcaactgg tcacgaattt gtgccgcttc ttcgaactcc
     5641 agattctgcg cgtgttgcat catcagccct tccagttcat ggattttctg ctgcaacgct
     5701 ttcggcgaca tatccatcgg cacattatcc ggctcaacaa tcgggcgcga ttttcctctg
     5761 cccttcgctt tggttttggc aatgttctgc cccagcgcca ggatatcgac cactttcttg
     5821 ttcaagcctt gcggcgtaat gccgtgttcc tcgttgtact tctgctgttt ctcacggcga
     5881 cgttcggttt cgccaatcgc tttcgccatt gatggggtga tcttatcgcc gtagagaatc
     5941 gctttaccgt taacgttacg tgccgcacga ccaatggtct ggatcaacga acgttcggaa
     6001 cgcaggaagc cttctttgtc agcgtcgagg atcgccacca gcgacacttc cggcatatcc
     6061 agaccttcgc gcagtaagtt gatccctacc agcacgtcga actcacccag acgcaagtcg
     6121 cggataatct ccatacgttc gacggtgtcg atatctgagt gaagataacg cacgcgctcg
     6181 ccatgttctt cgagatattc ggtaagatct tccgccatcc gcttggtcag tgtggtgacc
     6241 agtacgcgtt cgttaattgc cgctcgctga cgaatctccg aaagaagatc atcaacctgt
     6301 gtcgccaccg gccgcacttc gataatcggg tcaagcaatc cggttggacg caccacctga
     6361 tccaccacat cgccgccgga tttttccagc tcgtaattac ccggcgtcgc cgaaacatag
     6421 atggtttgcg gcgctaatgc ttcgaactct tcaaacttaa gcggacggtt atccagcgct
     6481 gatggcaggc ggaagccgta ctccaccagt gtctctttac gcgcccggtc accgcgatac
     6541 atgccgccaa tttgtggaat ggtgacgtga gattcatcga cgaccagcag cccatcggca
     6601 ggcaggtaat caaacagcgt cggcggtggc tcacccggtc cacgaccgga gaggaagcgc
     6661 gagtagtttt caatccccga acagtagccc agctcgttca tcatctccag atcaaactgg
     6721 gtacgctggg tcagccgctg ctcttccagc agtttgttgt tttccaacag cactttgcgt
     6781 ctggcggcca gttcttcttt gatctcctcc atcgcctgta cgatgcgctc gcgcggtgtg
     6841 acgtagtgcg ttttcgggta gatggtaaaa cgtggaatag tggaaacaat ctgcccggtc
     6901 agcgggtcaa ataacgacaa tcgttccact tcctcgtcaa acagttccac gcgaagtgca
     6961 atgtcatccg attctgccgg gaagatatca atcacctcgc cacgaacgcg gaaagtacca
     7021 cgctggaatg cttgatcatt acgagcgtat tgcagctccg ccagtcggcg cagaatcgcg
     7081 cgctgatcga taatcatacc gaccgtgaga tggagcatca tcttgagata taaatcagga
     7141 tcgcccagac cataaatcgc ggaaacagag gccactacaa ccacatcacg ccgctccagc
     7201 atcgctttgg tggcggataa acgcatctgc tcaatatgtt cgttaaccga ggcatctttc
     7261 tcaatgaaag tgtcggaact cggtacatag gcttccggct gatagtagtc gtagtaggag
     7321 acgaaatact ccaccgcgtt ttccgggaag aactctttca tttcgccata cagctgggcc
     7381 gccagcgttt tgttgggcgc aagtaccatg gttgggcgct gaaggtcagc aatgacattg
     7441 gcaatggtga aggttttccc tgagccagtc acgccaagta acgtctggtg cgccaggcca
     7501 tcttccagcc cctcttcgag acgtcgaatc gcctctggct gatcgccaga aggtttaaaa
     7561 gcggaattca gtttgaacgg tttactcatg agtcgctacc tgaaggagtt gggcgggcag
     7621 gtatgtaatt ttactcgtcg tacttaatta tgccaacaaa ttatactgga taaaaaaaca
     7681 gttcatcacc ataatatttc tgatacagcg taaactccgc tgtcaatcat gagcaaaatt
     7741 tactctgtgg cgagataaaa ctccggcctt accgggttat ccccaaagca acggcttttt
     7801 taacatttgt caagatgagt gatgacagtt ttatggcaag agatgcctgt tcagtgactg
     7861 ccattgcatt tatctaacca gttaaaaaat aaaagatatt tctttgagcc gtctttaacg
     7921 ccaattcgcg tgcaagccgc gtattctctc gcttgcctcg tgttttctaa ctctaataca
     7981 catggttatc cacaggaata gtggataact gtctccagcc cctatccccc gccgcctggg
     8041 caattcccac attcgccgca tgaggcggaa gtaaaatttt tttcgtgatt attttagaat
     8101 ttaattggtt aaattgcagt caatcgaaga cgcgatctcg ctcgcaattt aaccaaatac
     8161 agaatggcta caacaaggca aggtttatgt actttccggt tgccgcattt tctggatttt
     8221 ctgcaagcca ggggatctct cccagcagcg gcgcgggaat catgcgggtg agcgtggtca
     8281 tatattcagc gtgacgtttt cccggaggcg taacatcgtt cgccacccaa cccgccagag
     8341 tcagtccggc gtgttgtatt gcctgcgcag tcaacatcgc gtgattaata cagccgagtt
     8401 tcacaccaac taccagtatc accggcagtt gttcctgtgt tacccaatct gcaaaagtga
     8461 aagtgtcaga aagcggcgta aaccagccgc cagcaccttc cactaacacc cagtcagcct
     8521 gttgttcaag cgcgcgtaat ccggcgctca ttaccaatga ttctatcggc ctgccctctt
     8581 gcgcgctgat gatgtgcggc gaagtgggtt ctgcgaaggt gtaaggattt actgttgcgt
     8641 aatccagctg caggctgctg ttgtgctgta acgccagcgc gtcactatta cgtaagcctt
     8701 ccggggtctt ttcgctgcca gaggcgaccg gtttataacc tgccgtccga taacctactg
     8761 ccttcgcggc ttgtaaaagt gcacaactgg cgacggtttt ccccacttcg gtatccgttc
     8821 cggtgacaaa ataacgttta ctcacgagca atcactccca aaaaaagatg atacgtcaga
     8881 ggatatcgcc cctgttgttg cggccaggcc agttgcaatc gctgcaattg cgaacgcgtt
     8941 aatattcgcg ggtcgcgccc ttcatgaaga tgcgtagcac cgatgccttt cagcgaacgc
     9001 atggcactga gcgcatcatc aaaccatagt gtgatgggct gaatatgatg ttgataatgc
     9061 acgccgttca gcgactgttc gatttcatct ggcggtaaaa agcgattagc atgcggacgc
     9121 tcgtccaccg cctgccacgc ctgatgcagt tcgggtaacg atccctgcac cagcgtggta
     9181 aacgccacca cgcctccggg gcgcaccacc cgatacagct cgcggagtgc cgtggataaa
     9241 ttaccgcacc actgcaccgc gagattgctc catgcaagat cgaacgtcgc agtcgctaac
     9301 ggcagggatt cgatatctcc cgccagataa tggtctgcag catctttctg gcgggcttgc
     9361 acaagcattg gcggtgagag atctaacgcc gtcacctgtg cgtgacgctc tcgccagtgg
     9421 cggctcatcc agcctggccc acaccccgcg tccagtacgt gggtgtattt acgctgtggg
     9481 agcattgcca gtaagacgtc agcactccgg cgctgtaact ctgcgtgttg ctcatagtgc
     9541 gcggctgccc gaccaaatgc cgctgcaatg gcttgtttat taaccgttgc catgcagcac
     9601 ctccagcaga cggtcgatat cctgcatttc atgcgctgcg gttagcgtta agcgcagtcg
     9661 cgcagtacca gcgggtacgg ttggcgggcg aatcgccgtg acccagcagc cttgctggcg
     9721 cagtttttct gccagttgta acgcacggct gttatcaccg acgatcaatg gctggatggc
     9781 gctgcatgaa tcagcaagcg taaacggcaa atcctgtact ccggcacgaa aacgcgcaat
     9841 gagtgacacc agtttttcgc gccgtgcatc cccctcatca cggcgaatga ccgccagcga
     9901 cgcacgtaat gcctgcgcct gagcgggcgg catactggtg ttgtagataa ggtggcgggc
     9961 gaattgcagc agataatccg ccaccgtact ggagcaaagc accgctgccc cgctgacgcc
    10021 aaatcctttg ccaaaagtca ccaccagcaa ttctggtttt accttttgca gccagcagct
    10081 gccgcgcccc tgctccccga taacgcccgt gccgtgggca tcatcgacca tcaaccagcc
    10141 attgtgctgt tgcgttacct gctggatttc cgccagtggc gcactatcgc cgtccatgct
    10201 gaacacgcct tctgtcacca ccagttgctg ccccggacag ggggaagcaa gcagtcgcgc
    10261 cagatgagtg acatcgttat gaacaaaacg gcgaagctgc gacgggctta aactggcagc
    10321 ctccagcaat gaggcatggc taagccggtc agcgacaatg cggtcctctt ttgccatcat
    10381 cgcggcaatg accgcctgat tagcggcaaa accagagata aatagcagtg cccgcgaata
    10441 gcccagccac tcggccagtt cttcttccag cgcctgatgc gcaacgctat aaccgctgac
    10501 gtgaccggaa ccgccgctac cgacgccaaa ttgctccgca ccctgctgcc aggcacggat
    10561 aatttgcgga tgatggctta aacctaaata atcgttactg gaaaagttca gatactggcg
    10621 atcatccgcc accagccagc gtccggctcc ttgcgccact ggataacggc gacgcagggc
    10681 atcggcagca cgccgcgcat cgagcgccgc gttgattttc tcctgccagc tcatagtgct
    10741 gccgcgttgt aatattcgtc agtatccggg gtcatcagcg cctgttcaag acgttgctgt
    10801 tgttcgttat cccctgccag cacggcagtt tgctgcggat ttagccccag tttgcggaac
    10861 agttgcaggt ctttatcttc ttccggattc ggcgtggtca gcagtttgca accgtagaaa
    10921 atcgagtttg cgcctgccat aaagcacatc gcctgagtct gttcgttcat ctgctcgcgt
    10981 ccggcagaaa ggcgcacgta agaggtcggc atcatgatcc gcgcgaccgc aatggtgcga
    11041 ataaaatcaa aggcatcaac gtcatcgtta tcggcaagcg gcgtgccttt caccttcacc
    11101 agcatgttga ttggcacgct ttccggcggc gtcggcaggt ttgccagttg cagcaataat
    11161 ccggcgcgat cttttaccgt ttcgcctaag cccacaatgc cgccagaaca gactttgatc
    11221 ccggcatcgc gcactttttc cagcgtatcg aggcgttcct gataagtgcg ggtggtgatg
    11281 atattgccgt aaaactccgg cgaggtgtcg aggttgtggt tgtagtaatc cagcccggcg
    11341 ttcgcgaggc gctgcgcctg agattcactc aacgtgccca gcgtcataca cgcctccagc
    11401 cctaaatctt ttaccccctg caccatctgc tccaggtacg gcatatcgcg ttcgttggga
    11461 ttcttccacg ccgcgcccat gcaaaaacgc gtcgatcccg ccgctttcgc tttgcgcgcc
    11521 gactccagca cctgttcaac ttccatcaac cgctcggctt ccagaccggt tttatagcgc
    11581 gagctttgcg ggcaatattt gcaatcttcc gggcaagcac cggttttaat cgacagcaac
    11641 gtgctgacct gcacctgacg aggatcgaaa tgctggcgat gcacctgctg cgcttcaaac
    11701 agcagatcca gcaacggttt ttcaaataat tctgcgactt gcgacaatgt ccagcgtggg
    11761 cggtgagcca tggggcttct ccaaaacgtg ttttttgttg ttaattcggt gtagacttgt
    11821 aaacctaaat cttttcaatt tggtttacaa gtcgattatg acaaaggacg atcttgcctt
    11881 tgaccaacgc catatctggc acccatacac atccatgacc tccccgctgc cggtttatcc
    11941 ggtggtgagc gccgaaggtt gcgagctgat tttgtctgac ggcagacgcc tgattgacgg
    12001 tatgtcgtcc tggtgggcgg cgatccacgg ctacaatcac ccgcagctta atgcggcgat
    12061 gaagtcgcaa attgatgcca tgtcgcatgt gatgtttggc ggtatcaccc atgcgccagc
    12121 cattgagctg tgccgcaaac tggtggcgat gacgccgcaa ccactggaat gcgtttttct
    12181 cgcggactcc ggttccgtag cggtggaagt ggcgatgaaa atggcgttgc agtactggca
    12241 agccaaaggc gaagtgcgcc agcgttttct gaccttccgc aatggttatc atggcgatac
    12301 ctttggcgcg atgtcggtgt gcgatccgga caactcaatg cacaacctgt ggaaaggcta
    12361 tctgccagaa aacctgtttg ctcccgcccc gcaaagccgc atggatggtg aatgggatga
    12421 acgcgatatg gttggctttg cccgcctgat ggcggcgcat cgtcatgaaa tcgcggcggt
    12481 gatcattgaa ccgattgtcc agggcgcagg cgggatgcgc atgtaccatc cggaatggtt
    12541 aaaacgaatc cgcaaaatgt gcgatcgcga aggtatcttg ctgattgccg acgagatcgc
    12601 caccggattt ggtcgtaccg gcaaactgtt tgcctgtgaa tatgcagaaa tcgcgccgga
    12661 cattttgtgc ctcggtaaag ccttaaccgg cggcacaatg accctttccg ccacactcac
    12721 cacgcgcgag gttgcagaaa ccatcagtaa cggcgaagcc ggctgcttta tgcacgggcc
    12781 aacttttatg ggcaatccgc tggcctgcgc ggctgcaaac gccagcctgg cgattctcga
    12841 atctggcgac tggcagcaac aggtggcggc tattgaagta cagctgcgcg agcaacttgc
    12901 ccccgcccgt gatgccgaaa tggttgccga tgtgcgcgtg ctgggggcaa tcggcgtagt
    12961 cgaaacaact cgtccggtga atatggcggc gctgcaaaaa ttctttgtcg aacagggggt
    13021 atggatccgg ccttttggca aactgattta cctgatgccg ccctatatta ttctcccgca
    13081 acagttgcag cgtctgaccg cagcggttaa ccgtgcggtg caggatgaaa catttttttg
    13141 ccaataacgg gaagtcagcg tgtgggtttc tggctacact ttctgcaaac tagaaaggag
    13201 ggttcatgaa actcatcagt aatgatctgc gcgatggcga taagctgccg catcgtcatg
    13261 tctttaacgg catgggttac gatggcgata atatttcacc gcatctggcg tgggatgatg
    13321 ttcctgcggg aacgaaaagt tttgttgtca cctgctacga cccggatgcg ccaaccggct
    13381 ccggctggtg gcactgggta gttgttaatt tacccgctga tacccgcgta ttaccgcaag
    13441 ggtttggctc tggtctggta gcaatgccag acggcgtttt gcagacgcgt accgattttg
    13501 gtaaaaccgg atacgatggc gcagcgccgc cgaaaggcga aacccatcgc tacattttta
    13561 ccgttcacgc gctggatgta gagcatattg atgtcgatga aggagccagc ggcgcgatgg
    13621 tcgggtttaa cgttcatttc cactctctgg caagcgcatc gattaccgcg atgtttagtt
    13681 aatcactctg ccagatggcg caatgctatc tggtatcact taaaggtatt aaaaacaact
    13741 ttttgtcttt ttaccttccc gtttcgctca agttagtata aaaaagcagg cttcaacgga
    13801 ttcatttttc tatttcatag cccggagcaa cctgtgaaca cattttcagt ttcccgtctg
    13861 gcgctggcat tggcttttag cgtgacgctg accgcctgta gctcaacacc acccgatcaa
    13921 cgtccttctg atcaaactgc gcctggtacc tcttctcgcc cgattctgtc ggcaaaagaa
    13981 gcgcagaatt tcgatgctca acactatttt gcatccctga caccaggtgc tgcagcgtgg
    14041 aatccttccc cgattaccct gcctgcgcaa cctgactttg ttgtcggccc ggcgggcact
    14101 caaggtgtaa cgcataccac gattcaggcg gcggtagatg cggcaattat caagcgtacc
    14161 aacaagcgcc agtatattgc cgtgatgcct ggtgagtatc agggaacggt gtatgtccct
    14221 gctgctccgg gtggaattac tctgtacggt acaggtgaaa aaccgattga tgtgaagatt
    14281 gggctttccc ttgatggggg catgagccct gccgactggc gtcacgacgt caacccgcgc
    14341 ggcaaatata tgccaggtaa accagcgtgg tatatgtacg atagctgcca gagcaaacgc
    14401 agcgacagta tcggtgttct ctgctctgcg gtcttctggt cacaaaacaa tggcctgcaa
    14461 ctgcaaaacc tgaccatcga aaacacgctg ggcgatagcg tagatgcagg taaccatccg
    14521 gcggtggcac tgcgtactga tggtgaccag gtacagatta acaacgttaa cattctcggt
    14581 cgtcagaaca ccttctttgt caccaacagc ggtgtgcaga accgtctgga aacgaatcgt
    14641 cagccgcgta cgctggtgac caacagctac attgaagggg atgtggatat cgtttctggt
    14701 cgcggcgcag tggtgttcga taacaccgaa ttccgcgtgg tgaactcacg tactcagcaa
    14761 gaagcgtatg tgtttgcacc ggctacgctg tccaacattt actacggttt cctcgccgta
    14821 aacagccgtt tcaatgcttt cggtgatggt gtggcgcaac tgggccgctc gctggatgtt
    14881 gatgccaata ccaacggtca ggtggtgatc cgtgatagcg ccatcaacga aggttttaac
    14941 acggctaaac cgtgggccga tgcggtgatc tctaatcgtc cgtttgcggg taataccggc
    15001 agcgtagatg ataacgacga aatacagcgc aatctgaatg acactaacta caaccgcatg
    15061 tgggaataca ataaccgcgg cgtgggtagt aaagtggttg cagaggcgaa gaagtaagag
    15121 caattaacta tttgccggat gcggcgtaaa cgccttatcc ggcctacggt tcgatgcgat
    15181 ttgtaggtcg gataagatgc gcaagcatcg catccgacaa taagtgccga atgcgaccta
    15241 cattcacatg gcgcttttta catctgacgg tttttattga agttaatcaa actacccgcc
    15301 ttgataatct cgcgctcttc agcagtcaga ctttccatat agagcgtaat ttccgttacc
    15361 ggcgcatctt catggatcac ataaccttta aacgtcgtac ccggattatc cagcgccgct
    15421 ttaatgccag ggatgtaaat gtaatccccc acttcaaagg ttggcacttc cgccatctgc
    15481 agcggtaaca tcccccagtt gatgacgtta gaacggtagc gtttagtcgc gtactcttcg
    15541 gcaatattcg ccagaccgcc aatcacacgc tggcagctcg ccgcctgttc acgcgcagaa
    15601 ccatcgcctg gtttcaccgc atataccatg ctgccaattt cagtttgcag cggatcaata
    15661 tgctcctgac cagcaatctg cttaatgcgc gcaaacacct ctgtcagctc gctgacattc
    15721 cccgccagac gctgattttc cagctcagca gtcgctttac ttctgccaac ataaccggga
    15781 tcgcggcgtg acagggtaaa ctccgccaga ccaatcggat ttgaacgata agaagaggtt
    15841 tcaccggaag gaatcagttc gtcggtggtg gtcacttcgt cgaggatctt cgagcacact
    15901 ttcaggacga tattgtcagt cagcgcaccc aattccggcc agtctttaat gttcggtccg
    15961 taaatcagcg gttgctgagt tgcccctttc acaaagccct gataaacacg gtttttatac
    16021 ggcgttacat cgaaggcgta ctccggcacg ttgtcccagc aatcaagttc gctggcagag
    16081 gttaaatagc caccatttgc cgcagtcgca gcgatagaac gagcgtccat caacgccacc
    16141 gctgacatct gcccattagc tggcttagag ccttcgcggt tcgggaagtt acgcgtggtg
    16201 tggcgaatac ttaatccgtt gttgattggc gtatcgcccg cgccaaagca tgggccgcag
    16261 aacgcggttc tgatgattgc gcctgcgcca atcaaatctg ctaccacacc ttttttggct
    16321 aaatccataa acaccggctg tgatgacggg taaaccgcca gcgagaaggt gtcattgcca
    16381 caggattgac cgcgcagtgc attcgccgcc gcgatgacgt tttcgtagtt accgccagaa
    16441 cagcccgcga taatcccctg ctgcactttc aggcgaccat tttccacttt atccagcagc
    16501 gagagtttgg ctttaccgtg cgccacgcgt tcggactcaa tttcaatctc acgcagaatg
    16561 tcggtcaggt tctggttcag tgtgtcgatt tcatacacgt tgctcgggtg gaacggcagc
    16621 gcaatcattg gtttgatggc gcttaaatca acgctgatgc agccatcgta gtacgccatc
    16681 ggttgagggt taagctggca gtaatcctgg ccgcgaccgt gcagcgccag ccagttatgc
    16741 acttcttcat cagtttgcca gacggagctt aaacaggtgg tttccgtggt catcacgtca
    16801 acgctgttac ggaaatcggt agagagcgcg ctaacgcccg gtccaacgaa ctccatgact
    16861 ttgtttttga cgtaaccgtt tttgaacacc gcgccaatga tagccagcgc cacatcctgt
    16921 ggccccacat acggcgcagg ttttccggtc agatgcaccg caaccacgcc cggatagtcg
    16981 atatcccagg tgtcattaag cagctgtttt accaactcac cgccgccctc accgactgcc
    17041 attgtcccta atgcaccgta acgggtgtgg ctgtctgacc cgaggatcat tttgcccccg
    17101 cctgccatca tctcacgcat atattgatgg atgatcgcaa tatgcggagg cacaaaaata
    17161 ccgccataac gctgagctgc cgataaacca aaaacatggt catcaccgtt aatggtgccg
    17221 ccgacggcgc agagtgagtt atggcagttg gtcagcacat acggcagcgg gaaacgttcc
    17281 attccggacg ctttagcggt ctgtacaata ccaacaaagg taatatcgtg agaggctaat
    17341 gaatcaaact taattttaag tttatccata tttccggacg tattatgaga agagagaata
    17401 gaccaggcaa tagtgccttt tttggcttct tcttttttaa tttcgccggt gaaatgttct
    17461 tcggcaatta tttcgttatt actggcgaga aacacgcctt tt
//
LOCUS       DAZPEB010000073        17495 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZPEB010000073 DAZPEB010000000
VERSION     DAZPEB010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17495
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.074"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..642)
                     /locus_tag="ACHURJ_002136"
     CDS             complement(<1..642)
                     /locus_tag="ACHURJ_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFR1281080.1"
                     /translation="MSLNFTVIVWLRLSIGGVYELAFCRSSLKSKKYFIILLALAAIA
                     GLGTHAAWSSNGLPRIDNKTLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKG
                     TQDARELGNAFSADIPDFDLYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAI
                     KDLQSKAPDKNIVIFTHNHCLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDG"
     gene            893..2032
                     /gene="arnB"
                     /locus_tag="ACHURJ_002137"
     CDS             893..2032
                     /gene="arnB"
                     /locus_tag="ACHURJ_002137"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFR1281081.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            2036..3004
                     /gene="arnC"
                     /locus_tag="ACHURJ_002138"
     CDS             2036..3004
                     /gene="arnC"
                     /locus_tag="ACHURJ_002138"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFR1281082.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            3004..4986
                     /gene="arnA"
                     /locus_tag="ACHURJ_002139"
     CDS             3004..4986
                     /gene="arnA"
                     /locus_tag="ACHURJ_002139"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFR1281083.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VAHLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHATRQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISIAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            4983..5873
                     /gene="arnD"
                     /locus_tag="ACHURJ_002140"
     CDS             4983..5873
                     /gene="arnD"
                     /locus_tag="ACHURJ_002140"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFR1281084.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKTEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            5873..7525
                     /gene="arnT"
                     /locus_tag="ACHURJ_002141"
     CDS             5873..7525
                     /gene="arnT"
                     /locus_tag="ACHURJ_002141"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFR1281085.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            7522..7857
                     /gene="arnE"
                     /locus_tag="ACHURJ_002142"
     CDS             7522..7857
                     /gene="arnE"
                     /locus_tag="ACHURJ_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFR1281086.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            7857..8243
                     /gene="arnF"
                     /locus_tag="ACHURJ_002143"
     CDS             7857..8243
                     /gene="arnF"
                     /locus_tag="ACHURJ_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFR1281087.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(8237..8503)
                     /gene="pmrD"
                     /locus_tag="ACHURJ_002144"
     CDS             complement(8237..8503)
                     /gene="pmrD"
                     /locus_tag="ACHURJ_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFR1281088.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(8613..9968)
                     /gene="menE"
                     /locus_tag="ACHURJ_002145"
     CDS             complement(8613..9968)
                     /gene="menE"
                     /locus_tag="ACHURJ_002145"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFR1281089.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQY
                     ALVLEGENTFPALTSLHIQMVEGTHSALWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLLFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(9965..10927)
                     /gene="menC"
                     /locus_tag="ACHURJ_002146"
     CDS             complement(9965..10927)
                     /gene="menC"
                     /locus_tag="ACHURJ_002146"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFR1281090.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELLQMPSIAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALRLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(10927..11784)
                     /gene="menB"
                     /locus_tag="ACHURJ_002147"
     CDS             complement(10927..11784)
                     /gene="menB"
                     /locus_tag="ACHURJ_002147"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFR1281091.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(11799..12557)
                     /gene="menH"
                     /locus_tag="ACHURJ_002148"
     CDS             complement(11799..12557)
                     /gene="menH"
                     /locus_tag="ACHURJ_002148"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFR1281092.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(12554..14224)
                     /gene="menD"
                     /locus_tag="ACHURJ_002149"
     CDS             complement(12554..14224)
                     /gene="menD"
                     /locus_tag="ACHURJ_002149"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFR1281093.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPSLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(14313..15608)
                     /gene="menF"
                     /locus_tag="ACHURJ_002150"
     CDS             complement(14313..15608)
                     /gene="menF"
                     /locus_tag="ACHURJ_002150"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFR1281094.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLEEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(15687..15992)
                     /gene="elaB"
                     /locus_tag="ACHURJ_002151"
     CDS             complement(15687..15992)
                     /gene="elaB"
                     /locus_tag="ACHURJ_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFR1281095.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQVSDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(16047..16508)
                     /locus_tag="ACHURJ_002152"
     CDS             complement(16047..16508)
                     /locus_tag="ACHURJ_002152"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1281096.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLDT
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            16573..17490
                     /gene="rbn"
                     /locus_tag="ACHURJ_002153"
     CDS             16573..17490
                     /gene="rbn"
                     /locus_tag="ACHURJ_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFR1281097.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
BASE COUNT         4115 a         4696 c         4438 g         4246 t
ORIGIN      
        1 cccatccaga taaactttgc ctttttccac atgcatgact aaaccatcca gataatcagg
       61 tttaaatgtc gcatcacgct tatttttagc aatatatgtc aggcaatgat tatgggtgaa
      121 aataacgata tttttatcag gcgctttgct ttgtaagtcc ttaattgcac tataaatctc
      181 attaccgcac tgaagaagtc gtttatctac cgtcagcttt ttacccgcgg aaaaccaggt
      241 cgccgactga atggtccgga cggtattact ggaataaaga tcgaaatcag ggatgtcagc
      301 gctaaaagcg ttgcccagtt cacgggcatc ctgggtacct ttaaccgtaa tacctgtttt
      361 atctgacaag cattgattgg ttgaacgatc gcaacgttca gcatggcgaa acaaaacgac
      421 aaccgggtgc tgctgtgcca gtctggccaa tgttttattg tcgatacgtg gcaaaccatt
      481 gctactccag gcggcatgag tacccagtcc ggcaattgca gcgagcgcca gtaaaatgat
      541 gaaatatttt tttgacttca atgatgagcg gcaaaaagct aactcgtaaa ctccacctat
      601 agacaagcgc aaccagacaa ttaccgtgaa attgagcgac atttctggcg ataattagca
      661 gttggtgtaa tattaaaaat cctacgatgt cggcaaaatg cctcaaaatt ttgccaaatg
      721 caaagcctaa ataagaaaaa atataaaaat ttcaatattt acgtctaata ttagtttctt
      781 aaggttaagt taatattcta tccttaaaat ttcgttccaa atggcaaaat atacacaaca
      841 ctctttatag caaatataag tggacaggta ttcaatggcg gaaggaaaag caatgtcaga
      901 atttttgcct ttttcgcgac cagcaatggg cgtggaggaa ctcgctgcag ttaaagaagt
      961 tctcgaatcc ggttggatca caaccggtcc gaagaatcag gcgcttgagc aagccttttg
     1021 ccagttgacg ggaaatcagc atgccatcgc ggtcagttca gccaccgccg gaatgcatat
     1081 cacgctaatg gcgttggaaa ttggcaaggg cgatgaagtg attacgcctt ccctgacctg
     1141 ggtttcaacc ctcaatatga tttccttgtt gggtgcaacg ccggtaatgg tggatgtcga
     1201 ccgcgatacg ctgatggtca cgcctgaagc gatcgagtca gccattacgc cacgcactaa
     1261 agccatcatt ccggtgcatt atgccggtgc gccagcagat attgacgcca ttcgcgccat
     1321 tggcgaacgt tacggcatcg cagttatcga agatgctgcc catgccgtcg gtacgtatta
     1381 caaagggcga catattggcg caaaaggtac cgctattttt tcatttcatg ccattaaaaa
     1441 tattacctgt gcggaaggtg gcctgattgt aactgataat gaaaaccttg cccgccagct
     1501 acggatgctg aaatttcacg gcctgggtgt cgatgcctat gacagacaaa cctggggccg
     1561 agcaccgcag gctgaagtct taacaccggg ctataagtac aatctgaccg atattaacgc
     1621 cgcaattgcc ctgacacagt tagcaaaatt agagcacctc aacacccgtc ggcgcgaaat
     1681 tgcccagcaa tatcagcaag cactggctgc tctccccttt cagccattaa gccttcccgc
     1741 ctggccgcac gttcacgcct ggcatctgtt tattattcgt gtcgatgaac aacgttgtgg
     1801 tatcagtcgc gatgcgttga tggaagcgtt aaaagaaaga ggcattggta ccgggttaca
     1861 tttccgcgcc gctcacacac aaaaatatta tcgcgagcgt tttcccacgc tgtcgttacc
     1921 gaataccgaa tggaatagcg aacgcatctg ttctttgccg ctgttcccgg atatgactac
     1981 cgccgatgcc gaccgtgtca tcacagccct tcagcaactc gcaggacaat aagccatgtt
     2041 tgaaatccac cctgttaaga aagtctcggt ggttattccc gtttataacg agcaggaaag
     2101 cttaccggaa ttaatcaggc gcaccaccac agcctgtgaa tcgttgggga aagagtatga
     2161 gatcctgctg attgatgacg gcagtagcga taattccgcg catatgctgg tcgaagcctc
     2221 acaagcggag aacagccata ttgtgtctat tttgcttaac cgcaattacg ggcaacattc
     2281 agcgattatg gcgggattca gtcacgttac tggcgactta attattaccc ttgatgccga
     2341 tctccagaat ccgccagaag aaatcccccg cctagtggca aaagccgatg aaggttacga
     2401 cgtggtaggg actgtacgcc agaaccgcca ggacagctgg tttcgtaaaa cagcttcgaa
     2461 gatgattaac cggcttattc agcgcaccac cggcaaagcg atgggtgact acggttgtat
     2521 gctgcgcgcc tatcgccgtc atattgtcga tgcgatgttg cactgccatg aacgtagcac
     2581 ctttatcccg attctggcga atatcttcgc ccgccgtgcc attgaaattc cagtacatca
     2641 tgccgagcgt gagtttggtg aatccaaata cagttttatg cgcctgatta atttgatgta
     2701 cgacctggtg acctgcctta ccacaacgcc gctacgtatg ctgagtctgc tcggcagcat
     2761 tattgcgatt ggaggtttta gcattgcggt gctgctggtg attttacgcc tgaccttcgg
     2821 accacaatgg gcggcagaag gcgtctttat gctatttgcc gtgctgttta cttttattgg
     2881 cgctcagttt atcggcatgg gattactcgg tgaatatatc ggcaggatct acaccgatgt
     2941 ccgcgcccgc ccccgctatt ttgttcagca agttatccgt ccatccagca aggaaaatga
     3001 ataatgaaaa ccgtcgtttt tgcctaccac gatatgggat gcctcggtat tgaagctctg
     3061 ctggctgccg gttacgaaat tagcgccatt tttacccata ccgataatcc cggtgaaaaa
     3121 gccttttatg gttcggtggc tcatctggcg gcggaaagag gcattccggt ttatgcgccg
     3181 gataacgtta atcatccgct gtgggtggaa cgcattgccc aactgtcacc agaggtgatt
     3241 ttctcttttt attatcgcca tcttatttgc gacgagattt tgcagctcgc tcccgcaggt
     3301 gcatttaatc tgcacggttc gctgttacca aaatatcgtg gtcgcgcgcc gctgaactgg
     3361 gtgctggtga acggtgaaac ggaaacgggc gttacattgc accgaatggt aaaacgtgcc
     3421 gatgctgggg ccattgtggc gcaactgcgc gttgccattg cgccagacga tatcgctatt
     3481 acgctgcatc ataaattgtg ccatgccacg cgccagctac tggaacagac attacccgcc
     3541 attaaacacg gtaatattct ggaaatcgcc cagcgcgaaa acgaagccac ctgttttggt
     3601 cgcagaacgc cggatgacag tttccttgaa tggcataaac cggcatccgt actgcacaac
     3661 atggtacgtg ccgttgccga tccgtggccg ggtgccttca gctatgttgg caatcagaaa
     3721 ttcaccgtct ggtcgtcgcg tgttcatcct catgccagca aagcacagcc ggggagcgtg
     3781 atttctattg caccgctgtt gattgcctgt ggcgatggcg cgctggaaat cgtcaccgga
     3841 caggcgggcg acggcattac tatgcagggc tcgcaattag cgcagacgct gggcctggtg
     3901 caaggttcac gcttgaatag ccagcctgcc tgcaccgccc gacgccgtac ccgggtactc
     3961 atcctcgggg tgaatggctt tattggcaac catctgacag aacgcctgct gcgcgaagat
     4021 cattatgaag tttacggtct ggatattggc agcgatgcga taagccgttt tctgaatcat
     4081 ccgcattttc actttgttga aggcgatatc agtattcatt ccgaatggat tgagtatcat
     4141 gtcaaaaaat gtgatgtcgt cttgccgctg gtggcgatag ccacgccgat tgaatatacc
     4201 cgcaacccgc tgcgcgtatt tgaactcgat tttgaagaga atctgcgcat tatccgctac
     4261 tgcgtgaagt accgtaagcg aatcatcttc ccgtcaactt cagaagttta tgggatgtgt
     4321 agcgataaat acttcgatga ggaccattct aatttaatcg tcggcccggt gaataaacca
     4381 cgctggattt attcggtatc aaaacaatta cttgatcggg tgatctgggc ctatggcgaa
     4441 aaagaggggt tacagttcac cctcttccgc ccgtttaact ggatgggacc acgactggat
     4501 aaccttaatg cggcacgaat cggcagctcc cgcgctatta cgcaactcat tctcaatctg
     4561 gtagaaggtt caccgattaa gctgattgat ggcggaaaac aaaaacgctg ctttactgat
     4621 attcgcgatg gtatcgaggc gttataccgc attatcgaaa atgcgggaaa tcgctgcgac
     4681 ggtgaaatta tcaacattgg caatcctgag aacgaagcaa gcattgagga actgggcgag
     4741 atgctgctgg cgagcttcga aaaacatccg ctgcgccatc atttcccacc gtttgcgggc
     4801 tttcgcgttg tcgaaagtag cagctactac ggcaaaggat atcaggacgt agagcatcgt
     4861 aaaccgagca tccgcaatgc ccaccgctgc ctggactggg agccgaaaat tgatatgcag
     4921 gaaaccatcg acgaaacgct ggatttcttc ctgcgcaccg ttgatcttac ggataaacca
     4981 tcatgaccaa agtaggctta cgcattgatg tcgatacctt tcgtggcacc cgtgaaggcg
     5041 tgccgcgtct gctggaaatc ttgagtaagc ataatattca ggccagcatt tttttcagcg
     5101 tcggcccgga caatatgggc cgccatctct ggcgactggt gaagccacag tttttgtgga
     5161 agatgctgcg ctcaaacgcg gcatcgcttt atggctggga tattttactg gcaggtacgg
     5221 cctggccagg taaagagatt ggtcatgcca atgccgatat cattcgtgaa gcggctaaac
     5281 atcacgaagt cggcctgcac gcctgggatc accatgcctg gcaagcccgt agcggtaact
     5341 gggatcggca aacaatgatc gacgatattg cacgcggtct tcgcactctg gaagagatta
     5401 tcggtcaacc ggtaacctgt tctgccgctg cgggctggcg tgccgaccag aaggtgatcg
     5461 aagcaaaaga agcgttccat ttgcgctaca acagcgattg tcgtggggcc atgccgttcc
     5521 gtccattgct cgaatcagga aaccctggca ctgcgcaaat tccggtgacc ttacccacct
     5581 gggatgaagt gattggtcgg gatgtgaaaa cagaagattt taacggttgg ttactcaacc
     5641 gcatcctgcg agataaaggc acgccggttt ataccattca tgcagaagtt gaaggctgcg
     5701 cttatcagca taattttgtg gatctcctca aacgcgcagc tcaggaaggc gtgacatttt
     5761 gccctttaag cgaactgtta tcagagacgt tgccgctcgg acaagttgtt cgcggaaata
     5821 ttgccggacg tgaaggctgg ctgggttgcc aacaaattgc gggtagtcgc tgatgaaatc
     5881 ggtacgttac cttatcggcc tcttcgcgtt tattgcctgc tattacctgt taccgatcag
     5941 cacgcgtctg ctctggcaac ccgatgaaac gcgttatgcg gaaatcagtc gagaaatgct
     6001 ggcatccggc gactggattg tgccccatct gttagggcta cgttatttcg aaaaacccat
     6061 tgccggatac tggattaaca gcattgggca atggctattt ggcgcgaata actttggtgt
     6121 gcgggcaggc gttatctttg cgaccctgtt aactgccgcg ctggtgacct ggtttactct
     6181 gcgcttatgg cgcgataaac gtctggctct actcgccaca gtaatttatc tctcattgtt
     6241 tattgtctat gccatcggca cttatgccgt gctcgatccg tttattgcat tctggctggt
     6301 ggcgggaatg tgcagcttct ggctggcaat gcaggcacag acgtggaaag gcaaaagcgc
     6361 aggattttta ctgctgggaa tcacctgcgg catgggggtg atgaccaaag gttttctcgc
     6421 ccttgccgtg ccggtattaa gcgtgctgcc atgggtagcg acgcaaaaac gctggaaaga
     6481 tctctttatt tacggctggc tggcggttat cagttgcgta ctgacggttc tcccttgggg
     6541 actggcgata gcgcagcggg agcctgactt ctggcactat tttttctggg ttgagcatat
     6601 tcaacgcttt gcactggatg atgcccaaca tagagctccg ttctggtact acgtgccggt
     6661 catcattgcc ggtagcctgc cgtggctggg attactcccc ggtgcactgt acacaggctg
     6721 gaaaaaccgc aagcattccg caaccgtcta tttgttgagc tggacgataa tgccgctgct
     6781 gtttttctcc gtcgctaaag gtaaattgcc cacctatatt ctttcctgct ttgcatctct
     6841 ggcaatgctg atggcgcatt acgctttgct ggcagcaaaa aataatcctc tggcgctgcg
     6901 gattaatggc tggattaaca tcgcttttgg cgtcactggc attattgcca catttgtggt
     6961 ctccccgtgg ggaccaatga acacgccggt gtggcaaacc ttcgagagct ataaagtctt
     7021 ttgtgcctgg tcgatttttt cgctatgggc atttttcggc tggtacacct taacaaacgt
     7081 cgaaaagacc tggtctttcg ccgcgctttg cccgctgggg ctggcgttgc tggtaggatt
     7141 ttcaattcct gacagagtca tggaaggaaa acatccgcaa ttttttgtcg agatgacaca
     7201 agaatcactt caaccaagcc gctatattct tactgatagc gtcggtgttg ccgcaggtct
     7261 ggcatggagc ctgcaacgcg atgacatcat catgtatcgc cagacaggtg agttgaaata
     7321 cggccttaat tatccggatg cgaaagggag atttgtcagc ggtgatgagt tcgcaaactg
     7381 gcttaatcaa catcgtcagg aggggattat tacactcgtg ctttcggttg accgcgatga
     7441 agatatcaac agtctcgcca ttccgcccgc agatgccatc gatcgtcagg agcgtctggt
     7501 gctgattcag tatcgtccca aatgatctgg ctaacattag tctttgccag cttgcttagc
     7561 gttgccgggc agttgtgtca gaaacaggca acctgctttg tggcgataaa caaacggcgc
     7621 aaacatatcg tgctgtggct gggactggcg ctggcttgtc ttggtcttgc catggtgctc
     7681 tggctgctgg tcttgcagaa cgtaccggta ggcattgcct acccgatgtt aagtctgaat
     7741 tttgtctggg tgacgctggc tgcagtaaaa ctgtggcacg aaccggtatc gccgcgtcac
     7801 tggtgtgggg tggcgttcat tattggtggc attgtgatcc tcgggagtac ggtgtaatgg
     7861 gcctgatatg gggattattc agcgtcatta ttgcctcagt tgcgcaatta agcctgggtt
     7921 ttgcggcaag tcatctgccg ccgatgacgc acttatggga ttttatcgcg gctctgttag
     7981 cttttggcct ggatgcccgg atactgttac tcgggctgct gggttatctg ctttccgttt
     8041 tttgttggta taaaacgcta cataaacttg cgctaagcaa agcctatgcc ttgctcagta
     8101 tgagttatgt gctggtatgg attgcttcaa tggtacttcc cggctgggaa gggacttttt
     8161 cgctgaaagc actactggga gtagcctgta ttatgagcgg gttgatgctg attttcctgc
     8221 ccacgacaaa acaacgttac tgagttttcc ctgccgctgt acactgccgt tcccactcat
     8281 cggggctata actgcttgcc gagaggactt tcaccgtatg cagcttttca cgattaatac
     8341 aataaagcgc attctgcaaa ggcgagagta aatcccccac tttcacggcg aagtcactct
     8401 tcacttcggc gatcattttt atcgcgccgc cagcatcgca gagcataagt acatgtctgt
     8461 tatcttgttt attgcagcac gattttttga ccagccattc cattgcatta tcctgtttgc
     8521 taagagtttt cacatcaatt tacagattaa cagataaact ctttatcaac ggcaatggaa
     8581 tggcagaaat gagaagtgaa acggagggtt aattattgct gacgttgcac ccactctttt
     8641 agcgcctgac gtgaaatttt aataccgccg tttttcagct ccggcggtaa agtcagccag
     8701 cgcaccggtt gttgaaaacg agccagctta tctttcaccc attcgccaag atcaacggtt
     8761 tgctggtcat actccaccac cgctaccggc cgatgaccaa actccttgtc ggcaacggga
     8821 acgataaaca cctgcaaaac cgcaggatgt gcagcaataa cacgctcgac ttcttccggc
     8881 tgaataccct caccgccact gaataacaga ttgtctaaac gtccgacaat ggtcagcttg
     8941 ccattatgca tctcaccgcg atcgcgcgta gcgtaccagc cttcgtcatt aaccagtgaa
     9001 accagttgcc cgttacgcca gtaaccttct gccatactgg cagcccgcag ccacacttca
     9061 ttattaacga ttttcacttc ccgacccggc agcggcgaac caacgtctgc caggccgtcg
     9121 gcttctttcg cacataccgt ggaggcaaac tcggtcagac catagccgca aaagcaacga
     9181 atcccctgct cgcgcgcctg ttccgtcaac tcgaccggga tagccgcgcc gccaagtaac
     9241 accgctttca gggaaacgga actgcggtta accagcaaac gccagagttg tgttggcacc
     9301 agtgaagcgt gagtacagcc tgccagcatt tgctccaatg gctgtttatc acgcaccgtc
     9361 atccgcgcac cagcgtataa ccagcgccac ataattccct gaccggagac gtgaaacagc
     9421 ggtaaagaga gcaaccagtc atcgtgatcg ccaaacggaa tcagcgataa cacaccttgc
     9481 gcactggcaa gatgggcctg ataagtatgg acagcggctt tcggtaaacc ggtggagccg
     9541 gaggtcaatg tcattgaaca aagacgtgtt ggttgccaca gagctgaatg tgtgccttca
     9601 accatctgaa tatgcagtga agttaacgcc gggaaagtat tttctccttc cagcaccagc
     9661 gcatattgca gtgtaagatt tggcagtaat tcctcaagca acggttgcgg aagctgtgga
     9721 tttacgggca gcacccgcgc cccgcattgc agtaacgcca gccaggcgag cagcgtttgc
     9781 ggcgtattcc acgcccgcaa catcacgccg ctgccctcaa ccacaccctg cgccgcaaag
     9841 cccgtagcta actcatcgac gcgagcacaa agctcgcgcc agttgagttg ctcgtcatta
     9901 agacgtaagg cgatggcttc tccccgcact tgccgccagt gacgccacgg ccagtcagag
     9961 aagatcataa caaccgctcc agtgcatcaa cttccacgac aggcagcgtg ctacccggcc
    10021 agcgacgtac ctgctgcgcc tgcatcagat ccagcgtgtc cagccctgga atggtgtccg
    10081 gcgttaacca ggcggcaatc cgcgccagtt gcgttaagcc taagctcgac tcaatagaag
    10141 aactgatcac cgccgtcagc ctcaacgcgt gcgccgcctg tacctgctcg cgtacttttt
    10201 ccagactgcc cgtgagcgtg ggtttgataa ctaccgcgcg cacgccctct tcagccacaa
    10261 aggcaaaatc cggctcgcgc aggctttcat cccaggcaat ggcaatgccg gtttcacggg
    10321 caaacgctcg cgaatcatcg cgggttttgc acggctcttc gagaaacgcg atgcggtggc
    10381 gataatccgg gttaacatat ttggcaaact gctgaccttt cagcggtgtc caggcgcgat
    10441 ttgcgtcaag acgcaaatgc agatccggaa ttgcctccag caacagatta accaccatgc
    10501 cgtcgcgcac ggcttcgtac aatcccactt tgaccttcgc cactttctcg cctggcatat
    10561 cagcaagttt gaggatcaga tcgtcaggat cgccattaca cagtggtgcc gcacggtaat
    10621 tggccgcctg cgggagcgta tctgccagct ccgccaatgc acagcttacg ccaaaggcaa
    10681 tggaaggcat ctgcagtagc tcgcaatcgc ctgccagcca gttatttacc caggcaagca
    10741 gcacgctttg cgcctcttcc caggtttcct ggctgaagcc cggcagtggg gagatctccc
    10801 cccacccttc gcgctcgccc tcacgcaggc aaacgtacag cccgtcgcgg gtttttaacc
    10861 gcctgtcgcg cagaaccacc cccgcgtcca tggggatctg ccagcggtat acctgcgcgc
    10921 tacgcattac ggattccgtt taaatttgct gaaatcaggc tgacgtttct ggttgaaagc
    10981 gttgcgacct tcctgacctt cttccgtcat gtagaacagc atggtggcgt tgcccgccag
    11041 ctcctgcagc cccgcctgcc cgtcacagtc ggcgttcagt gcagctttca ggcagcgcag
    11101 cgccatcgga ctgttttgca gcatttcgcg gcaccagcga acggtctctt tttccagatc
    11161 cgccagcggt accacggtgt tgacaaggcc catatccagc gcctgtttcg cgtcgtactg
    11221 acggcacagg aaccagattt cccgcgcttt tttctgtccg acgatgcgag ccatgtagga
    11281 agcgccccag ccgccgtcga aggaaccgac tttcgggcca gtctgaccga agatggcatt
    11341 atctgccgcg atagtcaggt cgcacatcat gtgcagaacg tgaccgccgc cgatggagta
    11401 gccagccacc atcgcgacaa ccggtttcgg acaggtacgg atctgacgct ggaagtccag
    11461 cacgttcagg tgatgtacgc cggaatcatc tttatagccg ccgtaatcac cacgcacttt
    11521 ctggtcaccg ccggagcaga acgctttatc acctgcgcca gtcagaatga tcacgccgac
    11581 gttgtcgtca taacgcgcat ctgccagtgc ctggatcatc tcttttaccg tcagaggacg
    11641 gaaggcattg cgcacctgcg gacgattaat ggtgattttt gcgataccat cggtggattt
    11701 ttcataacga atgtcctcga aaccttcgga gcagtcgtgc cattcaaccg gtgcgtaaag
    11761 cattgcttca tcaggataaa tcatattgtg tcctttagtc agaaacgcaa gatctgcgcc
    11821 agacttgcga ttacgccagc gggattttcc cgatgcgcgt tatgtccggc gcgaggaatg
    11881 acatggcagt cggcagccag ttccgccgcc agggcgcgga atttgctgtc acgttcacca
    11941 cataaataat aaaacgcaaa tgtgcgggcg ctaaggttag cacgtaaatc aggctggacg
    12001 gcgagagaag tcgcctccag catggcggca agggttgcgc cattattgtt gctgcgcagc
    12061 gccaccagct cccggcgttg atcgtcattg agcgaggcaa aaacaggctg ttgataccag
    12121 tcggcaaata ccgccgttaa cggttctgtg cgaaaacgct gcgcccattg gcgatcggaa
    12181 cgctgacgtt ccgcacgttg ttcagcattt tgcagccccg gatgcccgcc ttcgacaata
    12241 accccacaaa gccccgccag cccctggcaa gccgccatca tcgccacccg tccgccaagc
    12301 gagtacccca ccagccagaa gtcaaggatg ttgtaactaa ccaaggtttt acgcagtaag
    12361 tcggtgacat catcaaatcc atcgacgcta atcgccgccg aaccaccatg acctgggaga
    12421 tcaacataca accgtgagta gtcggcaaac gcctcgccca cttcttgcca ttcgtggcaa
    12481 tcgccggaaa aaccgtgcaa aaacaccagc cagggtaaac ctggttttcc gtgttttgcc
    12541 tgcgcgtgca ggatcataaa tggcttacct gcgccagaag ttgctggagc gtttgcgcac
    12601 catcggtgtc gttaaccacc atttcaatca ccgtggtggt tggcgtgcgc caggcgtcgg
    12661 caaatgccgt ttcaagttcc tgccagtttt gcggacgatg atatttcagc tcgaacatcg
    12721 cggcggcgtg ctcaaaatgg acgttttgcg gcatcagata gaaacgctca cgctcgcttt
    12781 gcggcgttgg caacagcgag aaaatttgcc cgccgttgtt gttcaccaca attaatacca
    12841 gcggcgcaga aacctgacgc aataacgcca gcgcgttgag atcgtaaagt gcggagagat
    12901 cgcccacaat cgccagcgtc ggtttgccgc ttgcccgctg aacgcctgcg gcggtcgaaa
    12961 gcagtccgtc gataccgctg gcccctcggt tgctgtacac cgggtaacct gccggaagtt
    13021 gcgaaagcgc atcaatcaga cgtaccacca ggctgttacc gacaaacaat tgcccctgtt
    13081 caggcagata gtcgctgatg cgatgcgcca gttgcgcttc gccaaacgca tcgcgacggg
    13141 caataaccgc ctgcattgcc tgttccgcca ggcgcgggat ttcaacgcac cagggctggc
    13201 gtttttctgc cggatgcagc tccagccagt cggcaatatt ggcaattaag cggcgtccac
    13261 gatggtgcgc cggatcaagc cgcccttcga tgtcatccac aatccagtac tcttcgggtt
    13321 cacagcttgc ctgccattgc aggagccgtt tgcccgtcag gctgcttccc agttgcacca
    13381 caatttgcgc ctgctgcagc tcgctggtcg ctttggcatt gcctaaccaa agatcggcac
    13441 acggcagcgg ctgcccggtt tgtgacagca catcgccaat cagcggccag ccaagagttt
    13501 gcgcccacag ggcaactttt ttgccctctt ccgcactcat gcgcccggca accaccacgc
    13561 cgcgcttttg tcgccagaag aaccagtcgc gctgtttttc actttccaga ctaggcgctt
    13621 cacgcagcca cggtttgtcg tcctgccacc agtcacccag acgctgttgc cagctaagcc
    13681 cggtatcgtc catttcgcca tacagcggtt cagcaaacgg gcagttgata tggactcccc
    13741 ccgcatgaag cgtaccgaga gcgtggtcga tggtagaaac cagccaacgt gcggggatat
    13801 cctgggtcgg gcgcggcaat gaaatactgt gcgtggggtg agaggcgaac attcccggct
    13861 ggcgaattgc ctgattcgcg ccgcagtcaa ttagctccgg cgggcgatcg gcggttaaga
    13921 gaatcagttt ttctccggtt aacccggctt caatcagtgc cggatagaga tttgccaccg
    13981 cagtgccgga ggtcacaatc accgccaccg gctgcttgct gactttcgcc agccccagcg
    14041 ccagatgccc caacccacgc tcatcgaaat gggtgtggtg aatgaatgcg gaattctccg
    14101 ccgccgctaa cgttaacggt gtagaacgcg agcctggggc gatacagatg tgtctgacgc
    14161 cgtgacgcgt taatgcttcc agaatgaccg ccgcccagcg tcggttaaat gcgcttactg
    14221 acatgagttt gtccggtatc aatattgcgg ctaagtataa ggagctacaa aaatagatta
    14281 ttgatatgaa tcggtaatga tgcgactcat tactattcca tttgtaataa agtacgcaac
    14341 cctgccgctt tgttgtcgat ttcctgccac tcttgctcgg ggtcggaacc acggacaatg
    14401 cccgcgccag catataatcg cacgacattg ccgctaattt ttgctgagcg cagggaaacg
    14461 cagaattcgc tttgttgtaa tgagagatag cccgcagaac cggcgtacca ttctcgggtg
    14521 aacggttcgt gacgggcgat aaactgtcgc gccagatcgc gcggtaagcc agcaactgct
    14581 gccgttggct gcaactgatg taaacagatc acatcatccg ctttgttgag tgaagtccag
    14641 atacagcggc gaagatgctg cactttacgc agacgcagta cctgcggcgg taaaacatcc
    14701 agcgtctggg tatcggcctg taatcgttgg cagatatctt ccaccaccag catgttctcg
    14761 cgctggtttt tatcatccgc catcagccac tcttctaact gctgcgcctg cttatcatca
    14821 ggatgatttg ctactgttcc cgccagcgct tcagtacgca gcgctttgtc acgccgccgc
    14881 cataaccgtt ccggtgaaga gccaagaaaa gcattttcgc catcaaaggc catgtaaaaa
    14941 tggtagcaat tcagattcag tcgacgactg gcagccatca tcgccgccgc gttgaccgga
    15001 cttgcgaaat gcaggtcagt tgcccgagcg agcaccactt tgtcgagctc accttcggcg
    15061 atggttttcg ttgccagttc gattaattgc gtccagcccg ttttgtccgg ccagtgttgt
    15121 tctcgcgtgg tggttaaatg taacccaggc aagggcttga tactcaccag tgtggcgata
    15181 aattcttttg cctgaatcgc atcgtgctga agggagcttt cgctgaatag cgtcagccgc
    15241 agcgtggctt taccgccaca gcgtcgccat tccaggcggg gtaaaagtaa attgccctgc
    15301 gacgggtcaa aagcattcag cccccaaatg cgtaagtcgg cgtgttccgg gtgctggcga
    15361 agaaaacgtt gtgcctggtc caacgacgta aaacgggtaa tcgcgcccag gacggcagct
    15421 tcttcatcac cattacgttg ttgccagtag aattgcgggt acgtttgctg actggccagc
    15481 cagctcaagg catcaaaagc gtctttgaga gggaaaggaa tatcgataac ccgaatgccg
    15541 ggtgtcgccg gaatctcttg cgacaaatgg cgcagtagat tttccagcgc cgtagtaagt
    15601 gattgcacgc ggacctctcc ctgttaaaag cctcacatta tacggggtac tacaaaaaaa
    15661 atgcagtacc ccggtgtagg gaggttttaa cggcgtgcca gcaacagtcc tagtaccagc
    15721 ccaacggccg cgcccacacc aattccttgc caaggttttt cgtggacgta gtcatcagca
    15781 cgataaacag cctgcttcgc ccgatagtaa taactgtctg aaacctggct aacccgtttt
    15841 ttcacatcat ccagcgcttt ttctgcacgc gctttcagct caacatattt ctgatcagcg
    15901 ggatcgccag aggagcggag cacctcttcc agtgtctcac taagcagcgt caggtcgtca
    15961 tcgatacgtg tatcaccaaa ctgattagac attctcgttc tccatttgcg taaaacctgt
    16021 cactaactat agacaagggt tgttgattac gcctgaatta cctcgcgtgc catcccaatg
    16081 tgtgggatac catcttcttc atagacctcc gtcacaggga taaaaccgaa actctggtag
    16141 aagttttgca gatgcgcctg cgccccgagg taaacaggct tatcaggcca gtgatgcgtg
    16201 caggtgtcga gggttttact catcagttgc tgccccactt tttcgccacg caacgcttca
    16261 ctgacaatca cccgaccaat aacgaccggc tcaagatcat catcactttt cagaatcctc
    16321 gcatatgcca ccagttcatc atttttccaa ccgagaatat ggcgattatc ccccgtcaaa
    16381 tcatcaccat cgatatcctg ataagggcaa ttctgctcga caacaaacac cgcgcaacgc
    16441 agttgtaata aggcataaag ctgggaaacc gataattcag agtgatgcag atcttgccat
    16501 tcaatcataa tgctctcctt ttagatgtat accttatact agccggaata ttttttgaaa
    16561 cgtgatgaac tcatggaatt aattttttta ggtacttcag ccggtgtgcc aacccgcacg
    16621 cgcaatgtca cggcaatatt gcttaacctg caacatccga cccagagcgg actttggttg
    16681 tttgactgcg gtgaaggcac ccagcatcag ctactgcata ccgcctttaa ccctggaaaa
    16741 ctggacaaga tttttatcag tcaccttcat ggcgatcatc tttttggttt acccggcttg
    16801 ctgtgcagtc gttctatgtc aggcattatc caacccttaa cgatttatgg tccgcaaggt
    16861 atccgtgaat ttgtggaaac cgcgctgcgg attagcggct catggaccga ttatccgctg
    16921 gaaattgtcg aaattggcgc tggcgaaatt ctcgatgatg gcctgcgcaa agtaaccgct
    16981 tatccgctgg aacacccact ggaatgttat ggctatcgta ttgaagaaca tgataaaccg
    17041 ggtgcattaa atgcccaggc attaaaagct gctggcgtgc cgcctggccc actgtttcag
    17101 gaattaaaag cgggcaaaac aatcacgctg gaagatggaa ggcagattaa cggcgcagat
    17161 tacttagctg ctccagtgcc aggtaaagcg ctcgctattt tcggcgatac cggcccctgc
    17221 gatgccgcac ttgacctggc taaaggtgtc gatgtcatgg tgcacgaagc gacgctggat
    17281 ataaccatgg aagccaaagc caatagtcgc ggccatagct ctacacgcca ggctgcgaca
    17341 ctagcccgtg aggctggagt cggtaagcta atcattaccc acgtcagctc gcgctatgat
    17401 gacaaaggtt gtcagcacct gttacgtgaa tgcaggtcaa ttttcccggc gactgaacta
    17461 gcgaatgatt tcaccgtgtt taacgtttaa ctctg
//
LOCUS       DAZPEB010000074        17488 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZPEB010000074 DAZPEB010000000
VERSION     DAZPEB010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17488
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.075"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(39..2636)
                     /gene="topA"
                     /locus_tag="ACHURJ_002154"
     CDS             complement(39..2636)
                     /gene="topA"
                     /locus_tag="ACHURJ_002154"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFR1281098.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            3016..3267
                     /locus_tag="ACHURJ_002155"
     CDS             3016..3267
                     /locus_tag="ACHURJ_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFR1281099.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(3303..4352)
                     /gene="sohB"
                     /locus_tag="ACHURJ_002156"
     CDS             complement(3303..4352)
                     /gene="sohB"
                     /locus_tag="ACHURJ_002156"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFR1281100.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMGSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKY
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            4572..5330
                     /locus_tag="ACHURJ_002157"
     CDS             4572..5330
                     /locus_tag="ACHURJ_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFR1281101.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDICPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            5327..5918
                     /gene="cobO"
                     /locus_tag="ACHURJ_002158"
                     /pseudo
     CDS             5327..5918
                     /gene="cobO"
                     /locus_tag="ACHURJ_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
     gene            complement(5958..6830)
                     /gene="rluB"
                     /locus_tag="ACHURJ_002159"
     CDS             complement(5958..6830)
                     /gene="rluB"
                     /locus_tag="ACHURJ_002159"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFR1281102.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(7041..8936)
                     /locus_tag="ACHURJ_002160"
     CDS             complement(7041..8936)
                     /locus_tag="ACHURJ_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HFR1281103.1"
                     /translation="MAGKFRCILLLIAGLFVSSLSYAENPEIPSYEEGISLFDVEATL
                     QPDGVLDIKENIHFQARNQQIKHGFYRDLPRLWMQPDGDAALLNYHIVGVTRDGIPEP
                     WHLDWHIGLMSIVVGDKQRFLPQGDYHYQIHYQVKNAFLREGDSDLLIWNVTGNHWPF
                     EIYKTRFSLKFPDIAGNPFSEIDLFTGEEGDTYRNGRILEDGRIESRDPFYREDFTVL
                     YRWPHALLSNAPAPQTTNIFSHLLLPSTSSLLIWFPCLFLVCGWLYLWKRRPQFTPVD
                     VIETDVIPPDYTPGMLRLDAKLVYDDKGFCADIVNLIVKGKIHLEDQYDKNQQILICV
                     NEGATRNNEVLLPAEQLLLEALFRKGDKVVLTGRRNRVLRRAFLRMQKFYLPRKKSLF
                     YRPDTFLQWGGLAILAIILYGNLSPVGWAGMSLVGDMFIMICWIIPFLFCSLELLFAR
                     DDDKPCVNRAIITLFLPLICSGVAFYSLYINVGDVFFYWYMPAGYFSAVCLTGYLTGM
                     GYIFLPKFTQTGQQRYARGEAIVNYLARKEAATHSGRRRKGETRKLDYALLGWAISAN
                     LGREWALRIAPSLSSAIRAPEIARNGVLFSLQTHLSCGANTSLLGRSYSGGGAGGGAG
                     GGGGGGW"
     gene            complement(8964..9584)
                     /locus_tag="ACHURJ_002161"
     CDS             complement(8964..9584)
                     /locus_tag="ACHURJ_002161"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFR1281104.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(9581..10462)
                     /gene="rnm"
                     /locus_tag="ACHURJ_002162"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             complement(9581..10462)
                     /gene="rnm"
                     /locus_tag="ACHURJ_002162"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFR1281105.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLTTLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            10600..10644
                     /gene="trpL"
                     /locus_tag="ACHURJ_002163"
     CDS             10600..10644
                     /gene="trpL"
                     /locus_tag="ACHURJ_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFR1281106.1"
                     /translation="MKAIFVLKAWWRTS"
     misc_feature    10621..10714
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            10736..12298
                     /gene="trpE"
                     /locus_tag="ACHURJ_002164"
     CDS             10736..12298
                     /gene="trpE"
                     /locus_tag="ACHURJ_002164"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFR1281107.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLTLLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKNTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRTGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGIVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            12298..13893
                     /gene="trpD"
                     /locus_tag="ACHURJ_002165"
     CDS             12298..13893
                     /gene="trpD"
                     /locus_tag="ACHURJ_002165"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFR1281108.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQRKLEPTNTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGICFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            13894..15255
                     /gene="trpCF"
                     /locus_tag="ACHURJ_002166"
     CDS             13894..15255
                     /gene="trpCF"
                     /locus_tag="ACHURJ_002166"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFR1281109.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     GLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPTHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            15267..16460
                     /gene="trpB"
                     /locus_tag="ACHURJ_002167"
     CDS             15267..16460
                     /gene="trpB"
                     /locus_tag="ACHURJ_002167"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFR1281110.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVCEFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRETPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            16460..17266
                     /gene="trpA"
                     /locus_tag="ACHURJ_002168"
     CDS             16460..17266
                     /gene="trpA"
                     /locus_tag="ACHURJ_002168"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFR1281111.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLRDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
BASE COUNT         4223 a         4673 c         4347 g         4245 t
ORIGIN      
        1 gcgacccttt gtttataaaa acctgacaga attaaaggtt atttttttcc ttcaacccat
       61 ttgccatcaa cataaaatgc tgaccagcca gtcgcctttc cgtctttttc cgaagagaca
      121 tattgctgtt tggttttacg gctaaagcga accatggtct tattaccttc cggatcctgc
      181 tgtggcgcat cggccagata acgcagtttt tccggcagac ggtcgcggaa gcgataaagc
      241 tcttccacca gtggcgcacg cgtttcacgc gatttcggga aagtgttggc agccaggaaa
      301 acacccgcag cgccgtcacg cagcacgaaa taagcatctg atttttcgca cggcagctca
      361 ggtaatggca ccggatcttc tttcggtggt gccacttcgc cgttacgtaa aatcttacgt
      421 gtgtttttac actcttcgtt ggtgcaggcc atgtatttac cgaaacgccc cattttcagg
      481 tgcatttcag agccacattt ttcacactca acgatcgggc cgtcataacc tttaatgcgg
      541 aattcgccct cttcgatctc gtaaccgtcg caggttgggt tattaccaca gacatgcaac
      601 ttacgtttcg gatcgatgag atagctgtcc atcgccgtgc cgcatttcgg gcaacgacgt
      661 tttgcgcgca gcgcgttggt ttcagcgtct tcgccttcca gtacgttcag cacttcgttt
      721 tccggcacca ggttaatggt ggttttgcaa cgctctttcg gcggcagcgc ataaccagaa
      781 cagccaagga ataccccggt gctcgctgtg cgaatcccca ttttgcgacc acaagtcggg
      841 cagtcaatgc tggtcagaac catctggttc gggcgcatac caccctcttc cggatctttt
      901 tcagctttat ctaactgctg ggtgaaatcc gagaagaagt gatccagtac agctttccac
      961 tctgcttcgt gatttgccac ctggtcgagg ctgttttcca tctgtgcggt gaagtcgtag
     1021 ttcattaact cgcggaaatt ttcttcaagg cgatcggtga cgatttcgcc cattttttcc
     1081 gcatagaaac gacgattttc tactcgcacg tagccacgat cctgaatggt cgaaatgatc
     1141 gacgcatagg tagacggacg accgataccg cgtttttcca gctctttaac cagcgatgct
     1201 tcactgaaac gggctggcgg cttggtaaag tgctgggctg gtgtaagttc aacgagcgtc
     1261 agagcatcgc ctttattaac tgctggtaag atgcgatctt catcgccttt acgcaacgca
     1321 ggcatcactt ttgtccagcc atcaaaacgc aaaatacgac cgcgtgcttt caggcggaaa
     1381 tcgcccgcac caacggtcag cgtcgtggag tcatatttcg ctggggtcat ctggcaggct
     1441 acgaactgac gccagattaa ctggtacagt ttctgcgcat ctgcttccat atccttcagc
     1501 gattccgcca tcacattgac gtcagaagga cgaatcgctt cgtgcgcttc ctgtgagttt
     1561 tctttgctgg cgtactgatt cggactttcc ggcagatatt tcttaccaaa attatcgctg
     1621 atataaccgc gaaccatatt taccgcgtcc tgactcaggt tagtggagtc ggtacgcatg
     1681 taagtgatat agcctgcttc atacaaacgc tgcgccatca tcatggtttt tttcacgcca
     1741 aatccaagac gggtgctggc agcttgttgc agcgtagagg taataaaagg agcgccaggt
     1801 ttactggttg tcggtttgtc ttcacgttcc agcacgctgt agcgcgcttt ttccagcaga
     1861 cttaccgcag cctgagtttg ttctttgttg accggacgga acggtttgtc gttctgatga
     1921 gtcacctgta acgccaacgc ttcaccagat ggcgtggtcg tgctggcatc gacttcccag
     1981 aactcttccg gcacgaacgc tttaatttca cgctcacgct cgaccaccag gcgaaccgcc
     2041 accgactgca cacgaccggc agacagacca cgagcgatct ttttccatag cagcggcgaa
     2101 accatatacc ccaccacgcg gtccataaag cgacgcgcct gctgggcatt aacacgatca
     2161 atattcagct cacccggttt gttaaatgcc tggcggatcg cgtttttagt aatttcgtta
     2221 aacaccactc ggctatagcg cgcatcatca cccccaatca cttcccgcag gtgccatgca
     2281 atggcttccc cttcgcggtc aaggtcggtt gcgagataga tgtggtcggc tttttcagcc
     2341 agttgtttca gttcagagac gaccttctct ttaccaggca acacttcata gtgcgcctcc
     2401 caattgtgcc acgggtcaac ccccatacgg ttgacgagag cgccacgttc atccttttta
     2461 ggctttttag ccgtcttggt ggaggtagag tcggcactct ttttggcagc tgagccactg
     2521 gtcggcaaat cgcggatgtg accgacgctg gatttcacca cgtagtcact acccagatac
     2581 ttgttgatcg ttttggcttt tgccggggac tcaacgatga caagagcttt acccatattc
     2641 accttaccta atttgattct tccaggaatg cgtcgcacgt tgataaacct tccactggca
     2701 acgagttacc gatatggact cgttctctgc ggatatcaac cccttgtcgt gactctgttc
     2761 acgaaaattc aggtgactga gttaacaggt cttggcctac aggcgctata gcacgacgga
     2821 attttggtcg aatgtcaagc aaatctattg ccagattgac gaaagcgtca cactgtacct
     2881 gataaaatcc gatacgcaac tttattagca tgcaaaatcc aaagttgcca gtaaggcgct
     2941 cttgccacca gcgtggtata tttgtctgca agccatgcct ggcgtagact aatgtcctta
     3001 tttaaggagt aaataatgaa caaagagaca caacccatcg accgcgaaac gctgttgaaa
     3061 gaggccaaca aaatcattcg tgagcatgag gatacgctcg cagggattga agccaccggc
     3121 gtcacgcagc gtaatggcgt gctggtattt accggcgact actttttaga tgaacaagga
     3181 ttgccaacag ccaaaagcac cgcggtgttt aatatgttca aacacctggc ccatgtgctt
     3241 tcagaaaaat atcatctggt tgattaaagc aaaacgcgag gtcttagcct cgcgtttgtc
     3301 ttttacatca atggcttttg accccgctgc caccagcgta gcaacaatcg atcggcgctc
     3361 tctgccgcgc tgccggtgaa tcggtcaatg agtcgtttac gctgcatata gcgtacatta
     3421 accacttcac ggccttccat caggctaaga ataacttcat cactggtgtt gatttcatca
     3481 accaggcctt tctctaccgc ctgttgtccg taccagtgtt cacctgttgc cacctgttca
     3541 atatccagag acggacgcat acgcttcaca aaatctttaa acaactgatg cgtttcgttc
     3601 aactcttcgc ggaatttctc ccgcccttct tcggtatttt cacccagcaa cgtcagagta
     3661 cgcttatact gcccggcagt gtgcagttcg atatcaatat atttgctttt caggaagcgg
     3721 ttaaagttgg gcatttgcgc caccacccca atggaaccca caatagcaaa cggtgcggaa
     3781 acaattttgt ccgccacaca ggccatcatg taaccgccgc tggcagcgac tttgtctacc
     3841 gtaacagtta aaggaatgtt tttatcacgc agacgctgca gctgcgaagc cgccaacccg
     3901 taaccatgca ccatgccacc agggctttcc agacggagca caacctgatc ctgcggtttg
     3961 aatgctgcga gtaccgccgt tatctcttca cgtagcgagt tcacttcatg ggcgtccatg
     4021 ctgcctttaa aatccagcac ccagacgcga ggtttactgt cagttgccac ctcgcccagt
     4081 ttggctttcg cttttgctgc tttcgcttct tgcttgtgct ttttcttctg cgctttgtgc
     4141 cactgttttt gctgatgtga gcccatcagc gccgcggcca gttcttcttt catctcctta
     4201 tactgttcgc tgagattgtt gacccgtaac tcgccacgct ggcgtttatt acgttgagca
     4261 acattgacaa taatggcggc aatcgccgca atcgctagca caacggtaac gattttcgcc
     4321 aaaaacaaac catattcaga caacaattcc acgagtcccc accttggtta aacaacgctt
     4381 tggctcacag tttacaacag gcatgttaag ccgtctcgca gagatgttat tgcagcgaaa
     4441 gtatgacaaa gtatccattt tgcggtgtta tcgccccagg ctgattgaaa tgcccttctg
     4501 tttcaggcat aaagccccaa agtcataaag tacactggca gcgcgacaga caacgtcgtt
     4561 gaggagtagc catgcattac cagccaaaac aagatttact caatgatcgc attatcctgg
     4621 tgacgggagc cagcgatggg attggtcgtg aagccgcgat gacgtatgca cgctatggtg
     4681 cgacagtgat tctgttgggc cgtaatgaag aaaaattacg tcaggtagcc agccacataa
     4741 acgaagaaac tgggcgtcag ccacagtggt ttattctcga tttgctgacc tgcacgtccg
     4801 aaaattgcca acaactggca cagcgcattg tcgttaatta tccgcgtctg gatggtgttt
     4861 tgcataatgc cggattgctc ggcgatattt gcccaatgag cgaacaaaat ccgcaggtct
     4921 ggcaggacgt catgcaggtc aacgttaatg ccacctttat gctcacccag gcactgcttc
     4981 ctttattact caaatcggac gccggttcac tggtctttac ttcatcaagc gttggacgtc
     5041 agggacgagc taactggggt gcatatgcag cgtcgaaatt tgccaccgaa gggatgatgc
     5101 aggtactggc cgatgaatat cagcagcgcc tgcgcgtcaa ctgcattaac ccaggcggta
     5161 cgcgcaccgc aatgcgcgca agcgccttcc cgaccgaaga tccacagaaa cttaaaacac
     5221 ccgctgatat catgccgctc tacctctggc tgatgggcga tgacagccgc cgtaaaaccg
     5281 gcatgacctt tgacgcccaa ccgggccgta aaccaggaat ttcccaatga gtgatgaacg
     5341 ctaccaacag cgtcagcagc gagtgaaaga aaaagtagat gctcgtgtgg cacaggcccc
     5401 aggatgaacg cggtattatc atcgtcttta ccggcaatgg aaaaggcaaa accaccgcgg
     5461 catttggtac ggcaacacgc gcagttggtc acggaaaaaa agtgggcgtc gtgcagttta
     5521 ttaaaggcac ctggcctaat ggcgaacgca atctgctgga accacatggc gttgagtttc
     5581 aggtgatggc aacgggcttt acctgggata cacaaaaccg cgagtctgat accgccgcct
     5641 gccgcgaagt ctggcaacat gcaaagcgga tgcttgctga ttcctcactg gatatggttt
     5701 tgcttgatga actgacgtat atggtggcgt atgactattt gccactggaa gaagtggtgc
     5761 aggcgttaaa tgaacgtcca catcaacaga cggtgattat cacgggtcga ggctgtcatc
     5821 gggatattct tgaactggcg gatacggtaa gtgagctgcg ccccatcaaa catgcgtttg
     5881 atgccggtgt taaggcgcaa atagggattg attattaatc tgtaaaaatg ccggatatga
     5941 cgtccggcat actttgatta accgttatta cgcccgccag aacgacgacc gccgctcacc
     6001 tgactgtgac gtttcaccgc acgacgaatc tgattagcct tcatgcgacg acggtctttt
     6061 tctacagcga ctttagagct ggtttctggc ggaagctcca ccagttcgcg cagatagtta
     6121 gtctgggcga ggtccagctc cgtccagcca ccgcgtggca gaccttttgg cagtgggata
     6181 tcaccgtaac gaacacggat caggcggctc acctgcacac caaccgcttc ccacagacga
     6241 cgaacttcac ggttacggcc ttcggtcaga gtcacgttgt accactggtt gatcccttcg
     6301 ccgccgctga acttgatggt tttaaaagct gccggaccat cttccagttg cacgccacga
     6361 ctcaaatcac gcagtttcgc gtcgtcaacc tgaccaaata cacgcacggc gtattcacgt
     6421 tcaacttcac ggcttggatg cattaaacgg tttgccagtt caccatcggt ggtgaacagc
     6481 agcagaccac aggtgttaac gtccagacga cctacggcaa tccagcgtgc gccacgcagc
     6541 tttggcagac ggtcaaacac cgtcggacgc ccttccggat cgttacgggt acacaactca
     6601 ccttccggtt tgtaataggc cagaacgcga caaatttgtt cagcggactc acgtaccgaa
     6661 atcagatgac catcgatacg aattttcagc ccaggggtaa cttcaacgcg atcgccgagt
     6721 ttagcaattt tgccatcaac actcacacga ccggcttcaa taatagattc gatttcacgg
     6781 cgggagccgt ggccagcgcg ggccagcact ttctgtaatt tttcgctcat agagcttcct
     6841 tttggtgtcg ccttcccagg cgtctgatat gcttttgtgc aacagtgagt cagaacttac
     6901 atctgactca ctgatagtaa agtctgtttg aactcttcag gccttaagta aacgactcga
     6961 cgtttatcgg cctggctttg aggcgcaaca tactacacta tctttatggt taaagcttgt
     7021 ttcgattaac atcagcttaa ttaccagcca ccaccgcctc cgccacccgc gccgccgcca
     7081 gcaccaccac cggaataact tcgccccaac aaactggtat tggccccgca acttaggtgc
     7141 gtctgtaatg agaataaaac gccgttacgg gcaatctctg gagcgcgaat cgccgaagaa
     7201 agcgaagggg caatgcgtaa cgcccattcc ctccccaaat ttgccgatat agcccaacct
     7261 agcaacgcgt aatccagttt ccgtgtttcc cctttccgcc gacgtccact gtgtgttgct
     7321 gcctctttac gcgcaagata gttaacgata gcttcaccgc gggcataacg ttgctgccca
     7381 gtttgggtaa actttggcag aaaaatatac cccatgccag tgagataacc ggtcaggcaa
     7441 acagcgctaa aataacccgc tggcatatac cagtaaaaga atacatctcc gacattgata
     7501 tagagagaat agaaggccac gcctgaacaa atcagtggta aaaacaaagt gatgattgca
     7561 cgattaacgc aaggcttgtc atcatcgcgg gcaaacaaaa gctcaaggga acaaaataaa
     7621 aaaggaataa tccagcagat cataataaac atatcgccta ccagactcat tcctgcccaa
     7681 cctacgggac tcaggttacc gtagagaatg atcgccaata ttgccagtcc accccattgc
     7741 aaaaacgtat caggccggta aaacaaagac tttttacgcg gcagataaaa tttctgcatc
     7801 cgtaaaaatg ccctgcgtaa gactctgttg cgtctccccg taagaacgac cttatcgcct
     7861 ttacgaaata acgcttccag taataactgc tctgcgggca gtaatacctc attatttctg
     7921 gtcgcgcctt cattaacaca aatcaggatt tgctggttct tatcatactg atcttccaga
     7981 tgaatttttc ccttcacgat cagatttacg atatcggcac agaaaccttt atcgtcgtaa
     8041 accagcttcg catcgagacg taacatgccg ggcgtgtaat ctggcggaat gacatcggtt
     8101 tcaatcacat ctaccggcgt aaattgcggc ctgcgcttcc agagatataa ccatccacaa
     8161 accaggaata gacacggaaa ccaaattaac aacgatgacg tggagggtaa aagaagatgg
     8221 ctgaaaatat tcgtcgtttg tggagccggg gcattgctaa gtaacgcgtg aggccagcgg
     8281 tagagtaccg tgaaatcttc acgataaaac ggatcgcggg attcaattct tccgtcctca
     8341 aggatgcggc catttcgata tgtgtcgccc tcttctccag taaagagatc gatttcgcta
     8401 aatggattac ccgcgatatc agggaacttg agtgaaaatc gggtcttata gatttcaaac
     8461 ggccagtggt taccagtcac gttccagatt aacagatctg aatctccctc acgcaggaaa
     8521 gcatttttaa cctgataatg aatttgataa tgatagtcgc cttgaggcaa gaaacgttgt
     8581 ttatcgccca cgacaatact cattaacccg atatgccagt caagatgcca gggttcagga
     8641 ataccatcac gagtgacgcc aacaatatga tagttcagca gtgcagcgtc cccatcaggc
     8701 tgcatccaga gtcgtggtaa atcacgataa aatccgtgct taatctgctg atttcgcgcc
     8761 tgaaaatgaa tattttcttt gatgtcgagc accccatccg gttgcagagt ggcttcaaca
     8821 tcaaagagcg agatcccttc ttcataagaa gggatctccg ggttttctgc ataacttaga
     8881 gatgatacaa acagccctgc tatcaacagc aaaatgcagc gaaacttccc tgccatagtc
     8941 ttccatccgt ggaacgcaat ttgttataag aaaggcttca catcacccac gccttcacgc
     9001 accacgaccg gcgtatcatc ggtaagatca atgaccgttg tcggtttctg tccgagatag
     9061 ccgccgtgga taatcaaatc cacctgtttt tccagacggt ctttaatttc ttctggatcg
     9121 gattcggtaa attcgctgcc tggcagcatt agcgaagtgg aaagcatcgg ttcgcccagt
     9181 gcttcaagca acgcctgcgc aatcgggtta gaaggcacac gcataccgat ggttttgcgt
     9241 ttttcctgca acagacggcg tggcacctcc ttcgtcccct tcaggatgaa ggtatagttg
     9301 cccggcgtgt tgtttttcat taaacgaaac gcaacgttat ccacgaatga ataggtcgaa
     9361 agttcagaaa gatcgcgaca catcagggta aagttgtgac cgtccggcag ctggcgaata
     9421 cgacaaatac gctccatcgc gtttttatct tcaattttac agccgagcgc atagccggaa
     9481 tcagttggat aaacaatcac cccgccttta cgcacgatct ccaccgcctg gttgatcaga
     9541 cgttgctgcg ggttatcagg atgaatataa aaaaactggc tcataattcc ctctctgtgg
     9601 tgttctgcgg ctgttcccat agctgccaga cgccttcaac gcctgcgggc agccagagtt
     9661 tacgacccag ttcgatccac gggcatggct gatgaaaatc agatccttgt gatgcccata
     9721 aatgatgctg acgcgcaagt gtcgtcaact gggtgcgttc attgggcgat tgctggcact
     9781 gcgcgacttc catcgcgtcg ccgtggtgtt cggcaaaatg cgcaaccaat cttttcagcc
     9841 atttagcact aagattgtac cgcccaggat gagctagcac cgccttaccg ccagaatgat
     9901 gaatgacatc aatagcttgt tctattgtac accactgtgg cggaacgtat ccggttttcc
     9961 cgcgcgccag atacttttta aagacatctg ccattgaact ggctttgccg cactcaacaa
    10021 ggaaacgcgc aaaatgaccg cgcgtcactg cgccgccctg cgccagtcgt tgtgcgcctt
    10081 ccagcgcccc aggtatttgc gctttttcaa gtctttcggc aatttgctgc gcccgctgat
    10141 tgcgccgttc tgtctgctgc gcgaggaact cacacattag cggatgagta atatcaatat
    10201 tcagcccgac aatatgaatt tcatgatttt cccagaccgt ggaaatttcc acgccgggaa
    10261 taagattcag cgccagtccc gaacgtgaaa tttcctctct tgctggcgcg attgcagctg
    10321 tggtgtcatg gtcggtgatc gccagggtgc cgacgcgcat ctcgactgca cggtgcacca
    10381 atgcttctgg cgtcaggcag ccatcggaag ctgtggtatg gctgtgcagg tcgtaaatca
    10441 ctgcgtaatt cgtgtcgctc aaggcgcact cccgttctgg ataatgtttt ttgcgccgac
    10501 atcataacgg ttctggcaaa tattctgaaa tcagctgttg acaattaatc atcgaactag
    10561 ttaactagta cgcaagttca cgtaaaaagg gtatcgacaa tgaaagcaat tttcgtactg
    10621 aaagcttggt ggcgcacttc ctgaaacggg cagtatattc accatgcgta aagcaatcag
    10681 atacccagcc cgcctaatga gcgggctttt ttttgaacaa aattagagaa taacaatgca
    10741 aacacaaaaa ccgactctcg aactgctaac ctgcgaaggc gcttatcgcg acaacccgac
    10801 tgcgcttttt caccagttgt gtggggatcg tccggcaacg ctgctgctgg aatccgcaga
    10861 tatcgacagc aaagatgatt taaaaagcct gctactggta gacagtgcgc tgcgcattac
    10921 agcattaggt gacactgtca caattcaggc actttccggc aacggcgaag ccctgctgac
    10981 actactggat aacgccctgc ctgcgggtgt ggaaaatgaa caatcaccaa actgccgtgt
    11041 gctgcgcttc ccccctgtca gtccactgct ggatgaagac gctcgcttat gctccctttc
    11101 ggtttttgac gctttccgtt tattgcagaa tctgttgaat gtaccgaagg aagaacgaga
    11161 agcaatgttc ttcggcggcc tgttctctta tgaccttgtg gcgggatttg aagatttacc
    11221 gcaactgtca gcggaaaata actgccctga tttctgtttt tatctcgctg aaacgctgat
    11281 ggtgattgac catcagaaaa aaaacacccg cattcaggcc agcctgtttg ctccgaatga
    11341 agaagaaaaa caacgtctca ctgctcgcct gaacgatctt cgccagcagc tgaccgaagc
    11401 cgcgccgccg ctgccggtgg tttccgtgcc gcatatgcgt tgtgaatgta accagagcga
    11461 tgaagagttc ggtggcgtgg tgcgtttgtt gcaaaaagcg attcgcaccg gagaaatttt
    11521 ccaggtggtg ccgtctcgcc gtttctctct gccctgcccg tcaccgctgg cggcctatta
    11581 cgtgctgaaa aagagtaatc ccagcccgta catgtttttt atgcaggata atgatttcac
    11641 cctgtttggc gcgtcgccgg aaagttcgct caagtatgac gccaccagcc gccagattga
    11701 gatctacccg attgccggaa cacgcccgcg cggtcgtcgc gccgatggtt cactggacag
    11761 agacctcgac agccgcatcg aactggaaat gcgtaccgat cataaagagc tttctgaaca
    11821 tctgatgctg gtggatctcg cccgtaatga tctggcacgc atttgcaccc ccggcagccg
    11881 ctacgtcgcc gatcttacca aagttgaccg ttactcttac gtgatgcacc tggtctcccg
    11941 cgtggtcggt gagctgcgcc acgatctcga cgccctgcac gcttaccgcg cctgtatgaa
    12001 tatggggacg ttaagcggtg cgccgaaagt acgcgccatg cagttaattg ccgaggcgga
    12061 aggtcgtcgc cgcggcagct acggcggcgc ggtaggttat ttcaccgcgc atggcgatct
    12121 cgacacctgc attgtgatcc gctcggcgct ggtggaaaac ggtatcgcca ccgtgcaagc
    12181 cggtgctggc atagtccttg attctgttcc gcagtcggaa gccgacgaaa cccgtaataa
    12241 agcccgcgct gtactgcgcg ctattgccac cgcgcatcat gcacaggaga ctttctgatg
    12301 gctgacattc tgctgctcga taatatcgac tcttttacgt acaacctggc agatcagttg
    12361 cgcagcaatg ggcataatgt ggtgatttac cgcaaccata ttccggcgca gaccttaatt
    12421 gaacgcctgg cgacgatgag caatccggtg ctgatgcttt ctcctggccc cggtgtgccg
    12481 agcgaagccg gttgtatgcc ggaactcctc acccgcttgc gtggcaagct gcccattatt
    12541 ggcatttgcc tcggacatca ggcgattgtc gaagcttacg ggggctatgt cggtcaggcg
    12601 ggcgaaattc tccacggtaa agcctccagc attgaacacg acggtcaggc gatgtttgcc
    12661 ggattaacaa acccgctgcc agtggcgcgt tatcactcgc tggttggcag taacattccg
    12721 gccggtttaa ccatcaacgc ccattttaat ggcatggtga tggcagtacg tcacgatgcg
    12781 gatcgcgttt gtggattcca gttccatccg gaatccattc ttactaccca gggcgctcgc
    12841 ctgctggaac aaacgctggc ctgggcgcag cggaaactag agccaaccaa cacgctgcaa
    12901 ccgattctgg aaaaactgta tcaggcgcag acgcttagcc agcaagaaag ccaccagctg
    12961 ttttcagcag tggtacgtgg cgaactgaaa ccggaacaac tggcggcggc gctggtgagc
    13021 atgaaaattc gcggcgagca cccgaacgag atcgccggag cagcaaccgc gttactggaa
    13081 aacgccgcgc cgttcccgcg cccggattat ctgtttgccg atatcgtcgg caccggtggt
    13141 gacggcagta acagcatcaa tatttccacc gccagtgcgt ttgtcgccgc ggcctgtggg
    13201 ctgaaagtgg cgaaacacgg caaccgtagc gtctccagta aatctggttc gtccgatctg
    13261 ctggcggcgt tcggtattaa tcttgatatg aatgccgata aatcgcgcca agcgctggat
    13321 gagttaggca tatgtttcct ctttgcgccg aaatatcaca ccggatttcg ccatgcgatg
    13381 ccggttcgcc agcaactgaa aacccgcact ctgttcaacg tgctgggacc attgattaac
    13441 ccggcgcatc cgccgctggc gttaattggt gtttatagtc cggaactggt gctgccgatt
    13501 gccgaaacct tgcgcgtgct ggggtatcaa cgcgcggcgg tagtgcacag cggcggtatg
    13561 gatgaagttt cattacacgc gccgacaatc gttgccgaac tgcatgacgg tgaaattaag
    13621 agctatcagc taactgcaga agattttggc ctgacaccct accaccagga gcaactggca
    13681 ggcggaacac cggaagaaaa ccgtgacatt ttaacacgct tgttacaagg taaaggcgac
    13741 gccgctcacg aagcagccgt cgcggcgaat gtcgccatgt taatgcgcct gcatggccat
    13801 gaagatctgc aagccaatgc gcaaaccgtt cttgaggtac tgcgcagtgg ttccgcttac
    13861 gacagagtta ccgcactggc ggcacgaggg taaatgatgc aaaccgtttt agcgaaaatc
    13921 gtcgcagaca aggcgatttg ggtagaaacc cgcaaacagc agcaaccgct ggccagtttt
    13981 cagaatgagg ttcagccgag cacgcgacat ttttatgatg cgctacaggg tgcacgcacg
    14041 gcgtttattc tggagtgcaa gaaagcgtcg ccgtcaaaag gcgtgatccg tgatgatttc
    14101 gatccggcac gcattgccgc catttataaa cattacgctt cggcaatttc ggtgctgact
    14161 gatgagaaat attttcaggg gagctttgat ttcctcccca tcgtcagcca aatcgccccg
    14221 cagccgattt tatgtaaaga cttcattatc gacccttacc agatctatct ggcgcgctat
    14281 taccaggccg atgcctgctt attaatgctt tcagtactgg atgacgacca atatcgccag
    14341 cttgcagccg tcgctcacag tctggagatg ggggggctga ccgaagtcag taatgaagag
    14401 gaactggagc gcgccattgc attaggggca aaggtcgttg gcatcaacaa ccgcgatctg
    14461 cgtgatttgt cgattgatct caaccgtacc cgcgagcttg cgccgaaact ggggcacaac
    14521 gtaacggtaa tcagcgaatc cggcatcaat acttacgctc aggtgcgcga gttaagccac
    14581 ttcgctaacg gttttctgat tggttcggcg ttgatggccc atgacgattt gcacgccgcc
    14641 gtgcgccggg tgttgctggg tgagaataaa gtatgtggcc tgacgcgtgg gcaagatgct
    14701 aaagcagctt atgacgcggg cgcgatttac ggtgggttga tttttgtcgc gacatcaccg
    14761 cgttgcgtca acgttgaaca ggcgcaggaa gtgatggctg cggcaccgtt gcagtatgtt
    14821 ggcgtgttcc gcaatcacga tattgccgat gtggtggaca aagctaaggt gttatcgctg
    14881 gcggcagtgc aactgcatgg taatgaagat cagctgtata tcgatacgct gcgtgaagct
    14941 ctgccaacac atgttgccat ctggaaagca ttaagcgtcg gtgaaaccct gcccgcccgc
    15001 gagtttcagc acgttgataa atatgtttta gacaacggcc agggtggaag cgggcaacgt
    15061 tttgactggt cactattaaa tggtcaatcg cttggcaacg ttctgctggc ggggggctta
    15121 ggcgcagata actgcgtgga agcggcacaa accggctgcg ccggacttga ttttaattct
    15181 gctgtagagt cgcaaccggg catcaaagac gcacgtcttt tggcctcggt tttccagacg
    15241 ctgcgcgcat attaaggaaa ggaacaatga caacattact taacccctat tttggtgagt
    15301 ttggcggcat gtacgtgcca caaatcctga tgcctgctct gcgccagctg gaagaagctt
    15361 ttgtcagcgc gcaaaaagat cctgaatttc aggctcagtt caacgacctg ctgaaaaact
    15421 atgccgggcg tccaaccgcg ctgaccaaat gccagaacat tacggctggg acgaacacca
    15481 cgctgtatct gaagcgcgaa gatttgctgc acggcggcgc gcataaaact aaccaggtgc
    15541 tcggtcaggc tttactggcg aagcggatgg gtaaaaccga aatcatcgcc gaaaccggtg
    15601 ccggtcagca tggcgtggcg tcggcccttg ccagcgccct gctcggcctg aaatgccgta
    15661 tttatatggg tgccaaagac gttgaacgcc agtcgcctaa cgtttttcgt atgcgcttaa
    15721 tgggtgcgga agtgatcccg gtacatagcg gttccgcgac gctgaaagat gcctgtaacg
    15781 aggcgctgcg cgactggtcc ggcagttatg aaaccgcgca ctatatgctg ggcaccgcag
    15841 ctggcccaca tccttatccg accattgtgt gtgagtttca gcggatgatt ggcgaagaaa
    15901 cgaaagcgca gattctggaa agagaaggtc gcctgccgga tgccgttatc gcctgtgttg
    15961 gcggcggttc gaatgccatc ggcatgtttg cagatttcat caacgaaacc aacgtcggcc
    16021 tgattggtgt ggagccgggt ggtcacggta tcgaaactgg cgagcacggc gcaccgctaa
    16081 aacatggtcg cgtgggcatt tatttcggta tgaaagcgcc gatgatgcaa accgaagacg
    16141 ggcagattga agaatcttac tccatctccg ccggactgga tttcccgtct gtcggcccac
    16201 aacacgcgta tcttaacagc actggacgcg ctgattacgt gtctattacc gatgatgaag
    16261 cccttgaagc cttcaaaacg ctgtgcctgc acgaagggat tatcccggcg ctggagtctt
    16321 cccacgccct ggcccatgcg ctgaaaatga tgcgcgaaac cccggaaaaa gagcagctac
    16381 tggtggttaa cctttccggt cgcggcgata aagacatctt caccgttcac gatattttga
    16441 aagcacgagg ggaaatctga tggaacgcta cgaatctctg tttgcccagt tgaaggagcg
    16501 caaagaaggc gcattcgttc ctttcgtcac gctcagggat ccgggcattg agcagtcatt
    16561 gaaaattatc gatacgctaa ttgaagccgg tgctgacgcg ctggagttag gtatcccctt
    16621 ctccgaccca ctggcggatg gcccgaccat tcaaaacgcc actctgcgcg cctttgcggc
    16681 aggtgtgact ccggcacaat gttttgaaat gctggcactg attcgccaga aacacccgac
    16741 cattcccatt ggcctgttga tgtatgccaa tctggtgttt aacaaaggca ttgatgagtt
    16801 ttatgcccag tgcgagaaag tcggcgtcga ttcggtgctg gttgccgatg tgccagttga
    16861 agagtccgcg cccttccgcc aggccgcgtt gcgccataat gtcgcaccta tcttcatctg
    16921 cccgccaaat gccgatgacg acctgctgcg ccagatagcc tcttacggtc gtggttacac
    16981 ctatttgctg tcacgagcgg gcgtgaccgg cgcagaaaac cgcgccgcgt tacccctcaa
    17041 tcatctggtt gcgaagctga aagagtacaa cgctgcacct ccattgcagg ggtttggtat
    17101 ttccgccccg gatcaggtaa aagcagcgat tgatgcagga gctgcgggcg cgatttctgg
    17161 ttcggccatt gttaaaatca tcgagcaaca tattaatgag ccagagaaaa tgctggcggc
    17221 actgaaagtt tttgtacaac cgatgaaagc ggcgacgcgc agttaatccc acagccgcca
    17281 gttccgttgg cggcatttta actttcttta atgaagccgg aaaaatccta aattcattta
    17341 atatttatct ttttaccgct tcgcttaccc ctgtcgaacg tcaacttacg tcatttttcc
    17401 gcccaacagt aatataatca aacaaattaa tcccgcaaca taacaccagt aaaatcaata
    17461 attttctcta agtcacttat tcctcaga
//
LOCUS       DAZPEB010000075        17312 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZPEB010000075 DAZPEB010000000
VERSION     DAZPEB010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17312
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.076"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(11..820)
                     /locus_tag="ACHURJ_002169"
     CDS             complement(11..820)
                     /locus_tag="ACHURJ_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HFR1281112.1"
                     /translation="MTIEKSRFSSGAAPQPNVNTGVINGNDFAAIVPVIAGRIGGREA
                     NIVSAKALHKALGVGRDFTNWIKGRIDQYGFIAGTDFIRVENLSTPKRASAKTRQQIE
                     HDYLLSLDMAKEVAMVERNEQGRAIRRYFIQCEEELQRSVPEIAARYRRQLKARLSAA
                     NNFKPMCDALNMARAEMGKTTQQRHYSNESNMIARIVLGGMTAKQWAQANGITGEPRD
                     SMNAAQLEHLAYLESTNITLIDMGMEYEQRKGELTRLSQRWLAKRLEVNHA"
     gene            complement(834..1265)
                     /locus_tag="ACHURJ_002170"
     CDS             complement(834..1265)
                     /locus_tag="ACHURJ_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1281113.1"
                     /translation="MEKKNRPLQAANSDIRVSDVTPLTKSLQAPKRTPKKHRARVYML
                     RTGIEGWTENDILRYCRLSSGRNYASELERRLDIQLERIDEKNPDGIGSHLRYRFTGR
                     GDVLKVIRLVNHNADAGGYQGLSQPEIADILNLYPDNLTAA"
     gene            complement(1265..1468)
                     /locus_tag="ACHURJ_002171"
     CDS             complement(1265..1468)
                     /locus_tag="ACHURJ_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR1281114.1"
                     /translation="MNIDTIPTKGYIRRFRLAELLGVSVSTIDRKVRNGSLPRPVKLG
                     EKITAFDAVEIHNWLAERRGKVA"
     gene            complement(1581..2429)
                     /locus_tag="ACHURJ_002172"
     CDS             complement(1581..2429)
                     /locus_tag="ACHURJ_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1281115.1"
                     /translation="MKKLPPAPFFRLNRAAELLEIDIDDLLIMAAAESIYLSAYLCGY
                     DGTLHSIASLYRNCSLFPDVKEDCLLLADIITEVEDEKSFKAGVVNHIDCHVLGLWHV
                     NASYILDIAGLDSIDTPARIFSPAHRKRELDVSANYYFFLDYTKHAFHEISVEDLYIT
                     RPWVEYIGECMDKNQPIYSPETRINLQALYEDVKNVEHGNRERHRQKREICLLALIYV
                     KRHYPEECKGKNGKETNEAWANATINHWALLGGGYDEPSIDYLKKLIADADRTPEERT
                     TAGKKR"
     gene            complement(2525..3784)
                     /locus_tag="ACHURJ_002173"
     CDS             complement(2525..3784)
                     /locus_tag="ACHURJ_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1281116.1"
                     /translation="MPKLTDMQIRAWIKSGERFEGRADGNGLYLRYREADKTPTWRFR
                     YKLAGKSRAMLIGSYSELSLSKARETAKELSARVALGYDVAGEKQKRKTEALAKMEAE
                     KNAMRVSELAAEYFERQILPRWKHPDILRRRIDKDINPCIGSMKVEDVKPRHIDDMLK
                     GIVDRGAPTIATDVLRWTRRIFDYGIKRHALEINPCSAFEVADAGGKEAARDRWLTRD
                     ELIQLFKAMRTAKGFSRQNEITFKLLLALCVRKMELCAARWEEFDLDGAVWHLPEERS
                     KNGDPIDIPLPSPAVEWLRELHTFSCNSAWVLPARKMQNRMIPHIQESTLPVALAKVR
                     AEMPDVPNFTIHDFRRTARTHLAALGVDPVVAERCLNHRIKGVEGIYNRHQYFDERKA
                     ALAQWADLLVALESGKDYNVTPLRRAN"
     gene            complement(3975..4838)
                     /locus_tag="ACHURJ_002174"
     CDS             complement(3975..4838)
                     /locus_tag="ACHURJ_002174"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFR1281117.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDISAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            4965..5681
                     /gene="rph"
                     /locus_tag="ACHURJ_002175"
     CDS             4965..5681
                     /gene="rph"
                     /locus_tag="ACHURJ_002175"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFR1281118.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            5747..6388
                     /gene="pyrE"
                     /locus_tag="ACHURJ_002176"
     CDS             5747..6388
                     /gene="pyrE"
                     /locus_tag="ACHURJ_002176"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFR1281119.1"
                     /translation="MKPYQRQFIEFALGKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGLEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(6425..7021)
                     /gene="slmA"
                     /locus_tag="ACHURJ_002177"
     CDS             complement(6425..7021)
                     /gene="slmA"
                     /locus_tag="ACHURJ_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFR1281120.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(7128..7586)
                     /gene="dut"
                     /locus_tag="ACHURJ_002178"
     CDS             complement(7128..7586)
                     /gene="dut"
                     /locus_tag="ACHURJ_002178"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFR1281121.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(7564..8784)
                     /gene="coaBC"
                     /locus_tag="ACHURJ_002179"
     CDS             complement(7564..8784)
                     /gene="coaBC"
                     /locus_tag="ACHURJ_002179"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFR1281122.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEGLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSENNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            8956..9624
                     /gene="radC"
                     /locus_tag="ACHURJ_002180"
     CDS             8956..9624
                     /gene="radC"
                     /locus_tag="ACHURJ_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFR1281123.1"
                     /translation="MKNNSQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            9841..10077
                     /gene="rpmB"
                     /locus_tag="ACHURJ_002181"
     CDS             9841..10077
                     /gene="rpmB"
                     /locus_tag="ACHURJ_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFR1281124.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARSEKY"
     gene            10098..10265
                     /gene="rpmG"
                     /locus_tag="ACHURJ_002182"
     CDS             10098..10265
                     /gene="rpmG"
                     /locus_tag="ACHURJ_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFR1281125.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            10363..11172
                     /gene="mutM"
                     /locus_tag="ACHURJ_002183"
     CDS             10363..11172
                     /gene="mutM"
                     /locus_tag="ACHURJ_002183"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFR1281126.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(11211..11690)
                     /gene="coaD"
                     /locus_tag="ACHURJ_002184"
     CDS             complement(11211..11690)
                     /gene="coaD"
                     /locus_tag="ACHURJ_002184"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFR1281127.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(11698..12975)
                     /gene="waaA"
                     /locus_tag="ACHURJ_002185"
     CDS             complement(11698..12975)
                     /gene="waaA"
                     /locus_tag="ACHURJ_002185"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFR1281128.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQVLLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            13388..14446
                     /gene="rfaQ"
                     /locus_tag="ACHURJ_002186"
     CDS             13388..14446
                     /gene="rfaQ"
                     /locus_tag="ACHURJ_002186"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFR1281129.1"
                     /translation="MDKLFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLRVDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            14443..15567
                     /locus_tag="ACHURJ_002187"
     CDS             14443..15567
                     /locus_tag="ACHURJ_002187"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFR1281130.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     YPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYHLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            15560..16357
                     /gene="rfaP"
                     /locus_tag="ACHURJ_002188"
     CDS             15560..16357
                     /gene="rfaP"
                     /locus_tag="ACHURJ_002188"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFR1281131.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWSAIHRLRDVGVDTMYGVAFGEKGI
                     NPLSRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            16373..>17312
                     /gene="waaO"
                     /locus_tag="ACHURJ_002189"
                     /gene_synonym="rfaI"
     CDS             16373..>17312
                     /gene="waaO"
                     /locus_tag="ACHURJ_002189"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008918 - lipopolysaccharide
                     3-alpha-galactosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HFR1281132.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAIVSRFTY
                     MDQDILNLILLGKVKFIDAKYNTQFSLNYELKKSFVCPINDETVLIHYVGPTKPWHYW
                     AGYPSAQPFIKAKEASPWKNEPLMRPVNSNYARYCAK"
BASE COUNT         4149 a         4240 c         4438 g         4485 t
ORIGIN      
        1 ccacttcagg ttacgcatgg tttacctcca gacgtttagc gagccagcgt tgcgacaggc
       61 gggtgagttc tcctttgcgc tgctcatatt ccatgcccat atcaattaac gtgatattgg
      121 tgctttcgag ataggcgagg tgttcaagct gtgctgcgtt catgctgtcc cgtggttcgc
      181 cagtaatgcc atttgcctgc gcccactgct tcgccgtcat gccacccagc acgatacggg
      241 cgatcatgtt gctctcgttg ctgtagtggc gctgctgcgt cgttttgccc atctcggcac
      301 gggccatatt cagcgcatcg cacattggct taaagttgtt tgcggcactg agacgggctt
      361 ttagctggcg acgatagcgg gcggcgattt caggcacgct acgctgtaat tcttcctcgc
      421 actggatgaa ataacggcgg atagcgcggc cctgctcgtt gcgttcaacc attgccactt
      481 ctttagccat atccagcgat agcaggtaat catgctctat ttgctggcga gtttttgcgc
      541 tcgcccgttt tggcgtgctc aaattttcaa cacggataaa atcggttccg gcaataaaac
      601 cgtactggtc aattcggcct ttaatccagt tagtaaagtc gcggcccacc cccagcgctt
      661 tatgcaacgc cttagcgctc acaatattgg cttcacgccc gccaatgcgt ccggcaataa
      721 cgggaacaat agcggcaaag tcgttgccgt taattactcc cgtgttaacg ttgggttgcg
      781 gggcggcccc cgaactgaat cggctttttt cgatagtcat attttcggct ccgttatgcg
      841 gcggtgagat tgtccgggta tagattgaga atgtcggcaa tttcaggctg agaaagtccc
      901 tgatagccgc cagcgtcggc gttatggttc acaagccgaa taacttttag aacgtcaccg
      961 cgcccggtaa agcgatagcg taaatgtgac cctatcccgt cggggttctt ttcgtctata
     1021 cgctcaagct gaatatcaag cctgcgctct aactcgctgg cgtagttcct tccagacgaa
     1081 aggcggcaat aacgcagaat gtcattttct gtccatccct ctataccagt acgcagcata
     1141 tagactctgg cacgatgctt tttcggtgtg cgctttggtg cttgaaggga ttttgtaagg
     1201 ggcgtaacat cagatacgcg aatatctgaa ttagccgcct gtaatgggcg gtttttcttt
     1261 tccatcaggc caccttcccg cggcgttctg ccagccagtt gtgaatctca accgcatcaa
     1321 acgcagttat tttttctccc agctttaccg gacgtggtag tgaaccgttt cttactttac
     1381 ggtcgatggt ggatacacta acgcccagca attcagccag gcggaagcgt ctgatatagc
     1441 cttttgtagg gatagtgtcg atgttcatga ttcccgtaac ctcttagcga tagttgttgc
     1501 ttcgttacgg gtatttaatg gcgtgcacgc tcaaaaagga atgatcgtat tttttactat
     1561 cgtgatagta aaatttacta tcaccttttt ttccctgccg tggtgcgttc ttccggtgtt
     1621 ctgtctgcgt cggctatgag tttcttcagg taatctatgg atggctcatc atagccgccc
     1681 cccaaaagcg cccagtggtt tattgtggcg ttcgcccatg cttcgttggt ttccttcccg
     1741 ttcttccctt tacactcttc tgggtagtgc cgcttcacat aaatcaacgc caagaggcaa
     1801 atttcgcgct tttgcctgtg gcgctctctg tttccgtgct caacgttttt aacatcctca
     1861 taaagagctt gtaagtttat gcgagtttcg gggctgtata tgggctggtt tttgtccatg
     1921 cattcgccga tatattcaac ccacggcctt gttatgtaca aatcctctac ggatatttcg
     1981 tggaaggcat gttttgtata atcgagaaaa aaataataat tcgcacttac gtctagttct
     2041 ctttttctgt gtgcggggct gaatatgcgt gcgggagtgt caatagaatc aagccctgcg
     2101 atgtcgagta tataggaggc gttaacatgc catagcccaa gcacatggca atctatatga
     2161 tttacaacgc ctgccttaaa gcttttttcg tcctctactt cagtgattat gtcggcaagt
     2221 aataagcagt cttctttgac gtctgggaat aaagagcaat tgcggtaaag actcgcgatg
     2281 ctatgtaatg tgccgtcgta gccgcaaagg tacgcgctca aatatataga ttctgccgcc
     2341 gccataatca aaagatcatc aatatctatt tctaataatt cggctgctct gttaagcctg
     2401 aaaaaagggg cgggtggtaa ttttttcatt gtgtcacctt ttacgctgcg ccttattttg
     2461 tgattgagcc aggcggtaag gcttcccgct tttcgccccg tcgagctagg ctcaatctat
     2521 tcagttagtt cgcccttctg agaggcgtta cgttgtagtc ttttccgctt tccagtgcca
     2581 ccagcagatc agcccactgt gccagtgctg ctttacgctc atcaaaatac tgatggcggt
     2641 tataaatccc ctctacgccc ttaatgcgat gattgaggca tcgttccgcc acaacaggat
     2701 caacacccaa cgctgctaaa tgagtacgtg cggtgcgtcg aaagtcgtga atcgtgaaat
     2761 taggcacatc cggcatttcg gcgcgaacct tagccagtgc tacgggtaaa gtgctttcct
     2821 gaatatgtgg gatcattctg ttttgcatct tcctggccgg aagcacccat gcgctattac
     2881 atgaaaaggt gtgtagctct ctcaaccatt caacggctgg ggaaggtaga ggtatatcaa
     2941 tagggtctcc gtttttgctg cgttcttccg gcaaatgcca taccgcacca tctaaatcaa
     3001 actcttccca tcgtgcggcg cataattcca ttttgcggac gcataacgct aacagtaatt
     3061 tgaacgtgat ttcgttctgg cgactgaatc ccttagccgt gcgcatggct ttgaatagct
     3121 ggattaactc atcgcgggtt aaccagcggt cacgggcagc ttctttccct ccggcgtctg
     3181 ccacctcaaa ggctgaacag gggttaatct ctagcgcgtg ccgtttgatt ccgtagtcga
     3241 atatgcggcg cgtccatctc agcacgtccg ttgctatggt cggcgctcca cggtcaacaa
     3301 tacctttcag catgtcatcg atatggcgcg gtttcacgtc ctctaccttc atgctgccaa
     3361 tgcaggggtt tatatctttg tcgatacggc ggcggagtat atcggggtgc ttccagcgcg
     3421 ggaggatctg acgctcaaag tattcagcgg caagctctga aacgcgcata gcgttcttct
     3481 ctgcttccat cttcgccagt gcttcggttt tgcgcttctg cttctctcct gcaacgtcat
     3541 agcccagcgc aacgcgagcc gatagctctt tggctgtctc tctggccttt gatagtgata
     3601 gctcgctata cgaaccaatt agcatggcgc gggacttccc tgcgagttta tagcggaatc
     3661 tccatgtggg ggttttgtcg gcttcacggt aacgtaggta taaaccgtta ccgtctgccc
     3721 gcccctcgaa tcgctctccg ctcttaatcc atgcgcggat ctgcatgtct gttagttttg
     3781 gcattttgcg aatatccaaa ctttgaatag ggggtgtacc caaagcaatg tacccaaacc
     3841 tcattgggta cacctttgtg tacaccataa tcatgcgctt tgttgcgtct ttatgatagc
     3901 ccatgataac aaaaaagccc gctgggttgc gggcttggtg tgatttatgc gttgtcctaa
     3961 atgttgctga tagcttactc gatattctga atctgctcgc gcatctgctc aatcaacact
     4021 ttcagctcaa tcgcggagtt tgtcacttcg gcattgatag acttcgacgc aagagtgttc
     4081 gactcgcggt tgaattcctg catcataaag tccagacgac gaccaacggc ttcttttttc
     4141 ttcagaatgt tgtaggtctc tttgacatgc gcttccaggc ggtccagttc ttcggcaacg
     4201 tcaattcgtt gtgccagcag aaccagctcc tgctccagac ggttgttttc caactgaacc
     4261 tgagcatctt ccagcttcgc gaccagacgc tcacgctgcc attgcaggat ttccggcata
     4321 tgggcgcgga ctttgaccac ttcggcggtg acgccttcca gacgctgctc gatcaatgct
     4381 ttcagtgcct gaccttcggt ttcgcgcgcg acaataaagt cgtccagcgt accatccagc
     4441 gccgcgagaa tttcagcggc aatggcgtca agatcctgct cctgggctgc catcacgccc
     4501 ggccagcgta gaatatcaac cgggttgatt tccccttcgt cactctgcat ttttacccag
     4561 ttcgcggcag ttaccagctg tttagccagt ttttcgttga ggatcagctc accttgcgcg
     4621 ctaatatctg gctcatagcg cagggtacat tccactttac cgcgcgtcag gcgagaacga
     4681 atacgctcgc gaacgacagg ttcaaggcta cggaactgct ccggcagacg aaagtaagtt
     4741 tccagataac gctggtttac cgagcgcatt tcccaggttg cgctccccca ttcacccttg
     4801 atttcacgcc gggcgtaggc ggtcatactg cggatcatag acgttcctgt ttataaaagg
     4861 agaggtggaa ggattatagc catcgatgcc ttgtcaggat aggaataacc gccggaagtc
     4921 cgtataatgc gcagccacat ttgtttcaag ccggagattt caatatgcgt ccagcaggcc
     4981 gtagcaataa tcaggtgcgt cccgttaccc tgactcgtaa ctatacaaaa catgcagaag
     5041 gctcggtgct ggtcgaattt ggcgatacca aagtgttgtg taccgcctct attgaagaag
     5101 gcgtgccgcg cttcctgaaa ggtcagggcc agggctggat caccgcagag tacggcatgc
     5161 tgccacgttc tacccacacc cgtaacgctc gtgaagcggc gaaaggtaag cagggtggac
     5221 gcacaatgga aatccagcgt ctgatcgccc gtgctcttcg cgcggcagta gatttgaaag
     5281 cgctgggtga gttcaccatt acgctggact gcgacgtgct tcaggctgat ggtggcacgc
     5341 gtaccgcgtc gattacgggt gcctgcgtgg cgctggcaga tgcgctacag aagctggtgg
     5401 aaaacggcaa gctgaaaacc aacccgatga aagggatggt agccgcagtt tctgtcggaa
     5461 ttgtgaacgg cgaagcggtt tgcgatctgg aatacgttga agactctgcc gcagagaccg
     5521 acatgaacgt agtgatgacc gaagacgggc gcatcattga agtgcagggg acggcagaag
     5581 gcgagccgtt cacccatgaa gagctactca ccttgttggc tctggcccga gggggaatcg
     5641 aatccattgt agcgacgcag aaggcggcgc tggcaaactg atttttaagg cgactgatga
     5701 gtcgcctttt ttttgtctgt agaaaagtaa gatgaggagc gaaggcatga aaccatatca
     5761 gcgccagttt attgaatttg cgcttggcaa gcaggtgtta aagtttggcg agtttacgtt
     5821 gaagtccggg cgcaaaagcc cctatttctt caacgccggg ctgtttaata ccgggcgcga
     5881 tctggcactg ttaggccgtt tttacgctga agcgttggtg gattccggcc ttgagttcga
     5941 tctgctgttt ggccctgctt acaaagggat cccgattgcc accactaccg ccgtggcgct
     6001 ggcggagcat catgaccttg acctgccgta ctgctttaac cgcaaagagg caaaagacca
     6061 cggtgaaggc ggcaatctgg ttggtagcgc gttacaagga cgcgtaatgc tggtagatga
     6121 tgtgatcacc gccggaacgg cgattcgcga atcgatggag attattcagg ctaatggcgc
     6181 gacgcttgct ggcgtgttga tttcgcttga tcgtcaggaa cgggggcgcg gcgagatttc
     6241 ggcgattcag gaagttgagc gtgattacaa ctgcaaagtg atctctatca tcaccctgaa
     6301 agacctgatt gcttacctgg aagagaagcc ggaaatggcg gaacatctgg cggcggttaa
     6361 ggcctatcgc gaagagtttg gcgtttaaag aaactcgccg gatgaaaagt catccggcgt
     6421 catattactg caactgtgcc gcaattagcg gccagcgggc gtcaaaatca tccgtcgggc
     6481 ggtatttaaa ttcgctgcgg acaaaacgtg acagcatacc ttcacagaag gccaggatct
     6541 ggcttgccag cagggtttca tcggtggtgt aaccttcacc ctcacgcatt ctcttttcac
     6601 gcaatacctg acgcagctgc gcttcaatac gctcgaacag ctggttgatg cgcccttgca
     6661 ggcgatcctg ttcaaacatt agcgcatgac cagtgaggat gcgggtcagg ccaggattac
     6721 gctcaccaaa accgagaagc agcaacacaa tcagacgcag gcgcgctgtg gtgtctttct
     6781 catctttcag aatcaggttg atgcgagtaa tcaggctatc ttcgataaac tcaatcaggc
     6841 tatcgaacat gcgggtctta ctggggaagt ggcgatacag tgccgcttcg gaaacgccga
     6901 cagaggcggc cagttttgcc gtcgtgatac gttggcttcc atcgctggat tccagcatca
     6961 gcgccagaga ctgaagtatt tcctcgcgac ggttcctttt cgcagtttgt ttttctgcca
     7021 tgttacaaaa tacccctgaa aataagcact tgccaggcgg cacccacgct atgaccgcaa
     7081 acgaaatgtt tgcggctatg ttatgacgtt attcggatgc gtatgtgtta ctgacgacca
     7141 gagtgaccaa agccgccttc accgcggtcg gtggcgtcga aatcttccac cagattaaat
     7201 tcagcctgta ctaccggaac aaaaatcatc tgggcgatgc gttcgccagg ttgaatggta
     7261 aagctgtcct gaccacggtt ccacacggaa atcatcaact ggccctgata gtcggaatcg
     7321 atcaatccta ccaggttacc aagcacgata ccgtgcttat gtcccaatcc ggagcgcggc
     7381 agcatcattg ccgccagtga aggatcggca atatgaatcg ccagcccggt cggaaccagc
     7441 gtagtgtcac ccggagccag ttctacggcg tcgtcgagac aggcacgcag gtcaagtccg
     7501 gcagagccag aggtggcata agtcgggagc ggaaattcct tcccaacgcg cgggtccaga
     7561 atcttaacgt cgattttttt catcataacg ggtcacgatc tcgtcgagta atagttggcc
     7621 aaggagctct ttgcgctcaa gcggtaagac tttatctccg tcctgccaga aaaggtgtaa
     7681 tgcgttgttt tcgctgttaa atccttgagt tggctgggaa acatcgttcg cgcagatcag
     7741 atcaaggttt ttacggatac gtttttgccg ggcgtattct tccacattat ttgtttcggc
     7801 ggcaaatcca acgacgtagg gtcgatggtc ttttagtgcg gcaacgcctg cgacgatatc
     7861 ggggttttta accattttta ttgttaattc atcaccctgc gtggcctgct ttttgatttt
     7921 ttctggggcc acggtagctg cgcgataatc cgccacggcg gcgcagccga taaaaatatt
     7981 ttgctgctgt acagaagcat tcacggcggc ttccatttcc agcgcggtca tcacatcaac
     8041 acgtttaaca aacggtggcg tcggtagtga aaccggacct gataccagcg tgacgttcgc
     8101 gccacgacgg gcagcggcgg cggcgatagc aaaacccatc ttgccggagc tgtgattaga
     8161 gatataacgc acgggatcga gcggttcacg cgtcggaccg gcggtaatca taatgttcag
     8221 atgtttcagg tcgttgacgg gcgaaaaatg cgccaccgcc atatccacaa tggttaacgg
     8281 atcgagcatt cgcccaggac cgatatcacc acaagcctga ctgccactgt ctggccccca
     8341 gatgagcaaa ccacgggaag caagcccctc taaattatgc tgcgtggcag cggcacggta
     8401 catctgctgg ttcatggcgg ggagcacggc tacaggcgca ggtgtagcca gacaaatcgt
     8461 cgataccagg tcattcgcca ttccggcagc aacacgggca atcaaatctg ccgtggcagg
     8521 ggcgagaatc actaaatcag cccatttacc cagctcaata tggcccatag cggcttcggc
     8581 tgccgggtcc agcaaactgt cggaaacggg ataaccagaa accgcctgca agctaagtgg
     8641 ggtgataaag gcttttgccg cttcggtcat ggctacgcgg acgtcggccc cgcgatcgcg
     8701 caaacgacgc accagttcag gggttttata ggcagcaata ccgccgctaa cgccgagaac
     8761 gattttttta ccggccaggc tcatgatgat ttttcctgtt gggtgacacc agaagttggc
     8821 gattttatca caatactttt gttgtcgtgc cttcacctga gaatcacttt gcgaggcgct
     8881 ttccaggatt gaaaactggc cgtcgattta acggaacggc tatgacagga tgcgagcacc
     8941 acaaaggagg tgaaggtgaa aaacaattca cagctgttga tgccgcgcga aaaaatgctg
     9001 aagtttggta ttagcgcctt aacggatgtc gagctgctgg cgctatttct gcgtaccgga
     9061 acgcgcggta aagatgtatt aaccctggca aaagagatgc tggagaattt cggctctctt
     9121 tatggcttgt taacctctga atatgaacaa tttagtggcg ttcatggaat tggcgtggcg
     9181 aaatttgccc agttaaaggg gattgctgaa ctggcgcggc gttactacaa cgtgcggatg
     9241 cgtgaagaaa gccctttact cagcccggag atgacgcggg aatttttaca aagccagctt
     9301 acgggtgagg agcgggagat ctttatggtg atctttctcg actcacaaca ccgggttata
     9361 acgcatagcc gtcttttttc cggcacgcta aaccatgttg aagtccatcc tcgggaaatt
     9421 atccgcgaag cgataaaaat aaacgcctcg gcgctgatcc ttgcacataa tcacccttcg
     9481 ggttgtgctg aacccagtaa agcggataaa ctcattactg aacggataat aaagagttgt
     9541 cagttcatgg atttacgcgt gctcgaccat atcgtgattg ggcgtggaga gtatgtttct
     9601 tttgccgaac gcggctggat ttaacccgct atgcgcgatc cttcgggatc tttgtctgtt
     9661 cgggacttga gcacatcgct gagtcagcgt atactacgcc acctttgaga atctcgggtt
     9721 tggcatttgg gcctggcaat cgagagttca catagaactg cgatgactgg gctgtaaagc
     9781 ctgacgaggc gccaataccc catacgaagc tcgagctaat ttgatttttg gagaatagac
     9841 atgtcccgag tctgccaagt tactggcaag cgtccggtga ccggtaacaa ccgttcccac
     9901 gcactgaacg cgactaaacg ccgtttcctg ccgaacctgc actctcaccg tttctgggtt
     9961 gagagcgaga agcgttttgt caccctgcgc gtatctgcta aaggtatgcg tgtaatcgat
    10021 aaaaaaggca tcgatacagt tctggctgaa ctgcgtgccc gtagcgaaaa gtactaagta
    10081 cttagaggaa ataaatcatg gctaaaggta ttcgtgagaa aatcaagctg gtttcttctg
    10141 ctggtactgg tcacttctat accactacga agaacaaacg tactaagccg gaaaaactgg
    10201 aactgaaaaa attcgatcca gttgttcgcc agcacgtgat ctacaaagaa gcgaaaatca
    10261 aataattctc gctttgatgt aacaaaaaac ctcgctccgg cggggttttt tgttatctgc
    10321 ttgcccccat attgactgca tctgttcatt cctggagatg ctatgcctga attacccgaa
    10381 gttgaaacca gccgccgcgg catagaaccg catctcgttg gtgcaaccat tcttcatgca
    10441 gtggtgcgca acggacgctt gcgctggccg gtttcagaag agatctaccg tttaagcgac
    10501 caaccagtgc ttagcgtgca gcggcgggct aaatatctgc tgctggagct gcctgagggc
    10561 tggattatca ttcatttagg gatgtctggc agcctgcgca tccttccaga agaacttccc
    10621 cctgaaaagc atgaccatgt ggatttggtg atgagcaacg gcaaagtgct gcgctacacc
    10681 gatccgcgcc gctttggtgc ctggctgtgg accaaagagc tggaagggca taatgtgctg
    10741 gcccatcttg gaccggagcc gcttagcgac gatttcaatg gtgagtatct gcatcagaag
    10801 tgcgcgaaga aaaaaacggc gattaaaccg tggctgatgg ataacaagct ggtggtaggg
    10861 gtagggaata tctatgccag cgaatcactg tttgcggcgg ggatccatcc ggatcggctg
    10921 gcgtcatcac tgtcgctggc agagtgtgaa ttgttagctc gggtgattaa agcggtgttg
    10981 ctgcgttcga ttgagcaggg tggtacaacg ctgaaagatt ttctgcaaag tgatggtaaa
    11041 ccgggctatt tcgctcagga attgcaggtt tacgggcgaa aaggtgagcc gtgtcgggtg
    11101 tgcggtacgc cgattgtggc gactaaacat gcgcagcggg caacgtttta ttgtcggcag
    11161 tgccagaagt aattcatgcg cgccggatcg cataccatcc ggcataaacg ctacgctaac
    11221 ttcgccatca gcgcctgatg gacattctcc ggcaggaaat gggtgacatc gccctgatgg
    11281 cgcgccacct ctttcaccaa cgatgaagag ataaacgacc actctttcga cggcatcaga
    11341 aacacacttt ccagttccgg cattaagtgg cgattcatat gcgccagctg catttcatat
    11401 tcaaaatccg ccaccgcacg caggccacga atcagcaccg tagcgtgttg attacgggcg
    11461 aagttcgcca tcaaatcact aaacccaact acttccacgt tccccagatg cgcggttgcc
    11521 tgctgtgcca gcgccacacg ctcttccagg gtaaacatcg gttttttact ggggctggcg
    11581 gcaatcgcca gaataacgtg atcgaacatt tgcgtggcgc gcgtcacgat atcgatatga
    11641 ccattggtaa tgggatcgaa agtacccgga taaatcgccc gtttttgcat aacaacctca
    11701 atgcgttttc ggtggcaggt aaggttccag cagttgaagc aggcgctgta gcgcgccctg
    11761 gttttgatac agtacttcaa cggcatgacg gccatagaaa ctacggtaat cggcgtcggt
    11821 gagtaaagag gaaacctctt ttgcaagcgt agtggcatcg gtaacggtaa tcagcccgct
    11881 tgcctgctcc agccgcgcgc aaatgtcttt aaaattaaaa gtatgcggcc ccatcaatac
    11941 cggaatagcg tgtgcggcag cttccagcgg attatgccca ccacgttcaa ccagtgaacc
    12001 gccaacaaag gcgagatcgg caatgccgta cagtaacatc aactcgccca tcgtatcgcc
    12061 aaccacaacc tgcgtgctgg tggaggggac ttcccctgaa gagcgtgtga tatagcttag
    12121 tccagcctgg cggacaaggt taatcgcatc cgggaagcgt tctggatgac ggggtaccag
    12181 gatgagcaat aaattcggga attgctgtaa caatacctga tgtgcggcga tcaccacact
    12241 ctcttcacct tcgtgagtgc tggtggcaat ccataccggg cggtgtggtg cccactggcg
    12301 gcgcagcgtc acggctttag cagccaactg cggcgttaca gaaatatcga atttcaggct
    12361 accggtaacg gttacctggt tattttttgc gcccagcgcc acaaaacgtg caccatcttc
    12421 ttcattttgg gcggcaatca gcgtaatacg acgcagcaag cgacggacga atttacccag
    12481 tttggcataa cctgcggccg agcgggcaga gagtcgcgcg ttcgcgatca ccagcggaat
    12541 tttacgttta tgtagcgccg caatcaggtt aggccatagt tcggtttcca taatcaacac
    12601 cagtttaggg tcgactttat tcaggaaacg attaagtgca tcgggcaggt catacggcag
    12661 ataaacgtgc tgaacatcct tcccgaaagc cgattgtacg cgctccgaac cggttggcgt
    12721 catggttgta acggtaatcg gtaaatcagg ataacgatga cgcagcgcgc gcaccaacgg
    12781 gatcgccgcc agagtttcac cgacggagac ggagtgcagc ataatgccgc ctggttttag
    12841 cggatggcgg taaaaaccgt aacgttcacc ccagcgtttt cgataggccg gagccttacg
    12901 tccgcgcacc cagagccgta tccagatcag cggctgaata aggtagagaa gggcggtgta
    12961 aagcaattcg agcatagtaa atagctgact tatggatgtg ctggggattc tatgtattta
    13021 gctgtggctt taccattact tttcccgttt ttgacttaaa tagcaattta cccgccaatt
    13081 cttgctgaac atctatactt tttttgtatt tttatgcgat tcattgattt ctgctgtacg
    13141 tgcaggaagg aatcgcagag caacagtgaa agaatatggt tgctaatgat tttatttgat
    13201 tgatttcttt attgaaaatc aacaatgtgc ttaaaaatca ttcgggtcgg attgccgcga
    13261 aagaagtgat acactagcgc gtcaatatca gtgcggtatc agtattcagg tagctgttga
    13321 gcctggggcg gtagcgtgct ttttctgctt caccttaatc ggataatctc aacaaaaaga
    13381 gttacttgtg gataagctat ttcgaagaat tttgctcatt aagatgcgtt ttcatgggga
    13441 tatgttatta actactcccg tcattagttc gctgaaaaaa aattaccctg acgcaaaaat
    13501 cgatgtgctg ctttatcagg acaccatccc gatcctgtct gaaaatccag agattaacgc
    13561 gctctacggc ataaaaaata aaaaagcaaa agcctcagaa aaaattgcca acttttttca
    13621 tctcatcaag gtattacgtg ccaataagta tgaccttatc gtcaatctca ccgatcaatg
    13681 gatggttgct atactggttc gcttattaaa tgcccgtgtg aaaatttccc aggattatca
    13741 tcatcggcag tctgcttttt ggcgtaaaag tttcacccat ttggtgccgt tgcagggtgg
    13801 aaatgtggtg gaaagtaact tatccgtgct gaccccattg cgagttgatt cgttggtgaa
    13861 gcagacaacc atgagttacc cgcctgcaag ctggaaacgt atgcgtcgcg aacttgatca
    13921 tgctggtgtt ggacaaaatt atgtggttat ccaacctacg gcgcggcaaa tcttcaaatg
    13981 ctgggacaac gccaagtttt ccgctgtgat tgatgcctta catgctcgtg gttatgaagt
    14041 tgttctgacg tccggcccag ataaagacga tctggcctgc gtcaatgaaa ttgcgcaggg
    14101 atgccagacg ccaccagtaa cggcgctggc tggaaaggtg accttcccgg aacttggtgc
    14161 gttaatcgat catgcgcagc tgtttattgg cgttgattcc gcaccggcgc atattgccgc
    14221 tgcagttaat acgccgctga tatcgctgtt tggtgcgaca gaccatattt tctggcgtcc
    14281 ctggtcaaat aacatgattc aattctgggc gggagattac cgggaaatgc caacgcgcga
    14341 tcagcgtgac cgaaatgaga tgtatctttc ggttattccg gcggcagatg tcattgctgc
    14401 tgtcgataaa ttactgccct cctccacgac aggtacgtcg ttatgatcgt tgctttttgt
    14461 ttatataaat attttccctt tggcggtttg cagcgcgatt ttatgcgtat tgctcagaca
    14521 gtcgccgccc gaggtcatca tgttcgggtt tatacccagt cgtgggaagg cgaataccct
    14581 gatgtatttg aactgatcaa agtgccggtt aaatcgcata ccaatcacgg gcgcaatgcg
    14641 gagtattttg cctgggtgca aaaacattta cgcgaacatc ccgtcgataa agtcgttgga
    14701 ttcaacaaaa tgccggggct ggacgtttat tatgccgctg atgtttgtta tgccgagaaa
    14761 gtcgcgcagg aaaaaggctt tttctatcac ctgacgtcac gttatcgcca ttatgccgcc
    14821 tttgagcggg caaccttcga acagggcaag ccgacacagc tgctgatgct gacagataag
    14881 caaatcgccg atttccagaa acattatcag actgaagcgg agcgttttca tattctgcca
    14941 ccggggattt atcctgatcg taaatatagc cagcagccag ctaatagccg tgaaatcttc
    15001 cgtaagaaga atggaataac cgaacaacaa tatttattgt tgcaggtcgg ttcagacttc
    15061 acgcgtaaag gtgtcgatcg ttccattgaa gcacttgctt cgttaccgga ttcgctgcgc
    15121 cacaacacat tgctatatgt tgttggacag gataaaccgc gaaaatttga ggcgctggca
    15181 gaaaaacgcg gcgtgcgcag taatgttcac ttcttctcgg ggcgcaacga tgtctcggaa
    15241 ttaatggcgg cggcggattt attactgcat cctgcctacc aggaagcagc gggaattgtg
    15301 ctactggaag cgattactgc aggattaccg gtactaacaa cagccgtttg tggctatgcg
    15361 cattatattg tcgacgccaa ttgcggcgag gctattgctg agccattccg ccaggaaaca
    15421 ttgaatgaga ttttacgcaa agcgttaacg caatcttcat tgcgccaggc ctgggcggaa
    15481 aatgcgcgac attatgctga tacacaagat ttatacagtc tgccagagaa agcggcggat
    15541 atcataacgg gtggtctgga tggttgaact taaagagccg tttgccacgt tatggcgcgg
    15601 taaagatcct tttgaggaag ttaaaacctt gcagggtgag gtttttcgtg aactggaaac
    15661 tcgccgcact ctgcgatttg aaatggcggg caaaagctat tttctcaaat ggcatcgcgg
    15721 cacgaccctg aaagagataa tcaaaaattt actctcattg cggatgccag tattaggcgc
    15781 agaccgcgaa tggagtgcga ttcatcgact gcgggatgtc ggcgttgata ctatgtatgg
    15841 ggtggcattt ggcgaaaaag gtattaatcc gctgagcaga acctcgttta ttattaccga
    15901 agatctgacc ccaaccatca gtctggaaga ttactgtgct gactgggcga ccaacccacc
    15961 agatgttcgc gtaaagcgta tgcttattaa gcgtgtcgcg acgatggtgc gcgatatgca
    16021 tgctgcgggc attaaccacc gtgactgtta tatctgtcat ttcctgctgc acttgccttt
    16081 ttccggtaag gaagaggagt taaaaatttc ggtaattgac ctacaccggg cgcagcttcg
    16141 cacgcgcgtt ccacgtcgtt ggcgcgataa agatcttata gggctttatt tttcttcaat
    16201 gaatattggc ctgactcagc gggatatctg gcggtttatg aaagtgtatt ttgccgcccc
    16261 gcttaaagac attctcaagc aggaacaagg actgctgtcg caagcagaag caaaagccac
    16321 aaaaatcagg gaaagaacga ttcgaaaatc gttgtaattt ccggagagaa atatgagtgc
    16381 ccactatttt aatccacaag agatgatcaa taagacaatc atcttcgatg aaaggccagc
    16441 ggcgtcagtg gcatcatcat tccatgttgc ttatggcatt gataaaaact ttctttttgg
    16501 ttgtggtgtt tcaatcacgt cagttttgtt acataacaac gacgtgagtt ttgttttcca
    16561 cgtttttatt gatgatatcc ctgaagccga tatccagcgt ttagcccaat tggcgaaaag
    16621 ctatcgtacc tgtatccaga tccatctagt aaattgtgaa cggcttaagg cattaccgac
    16681 gaccaaaaat tggtctattg ccatgtattt ccgttttgta attgcagatt actttattga
    16741 tcaacaagat aagatccttt acctggatgc tgatatcgcc tgtcagggaa acttaaagcc
    16801 gctgataaca atggatcttg ccaataacgt tgctgctgtt gttactgaac gcgatgctaa
    16861 ctggtggtcg ttacggggtc aaagtctgca gtgtaatgaa cttgaaaagg gctactttaa
    16921 ttcaggtgtc ctgttaatta atacactagc gtgggcgcag gagtccgttt ctgctaaagc
    16981 gatgtcgatg cttgctgata aagccatcgt ttcccgtttc acctatatgg atcaagatat
    17041 ccttaatctt atcctgttag ggaaagttaa attcattgat gctaaataca atacgcaatt
    17101 tagtttaaat tatgaattaa aaaaatcatt tgtttgtcca attaatgatg aaaccgtatt
    17161 aattcattat gtcggcccga caaaaccctg gcattactgg gccggttatc caagtgcgca
    17221 accttttatc aaagccaaag aagcatcgcc ctggaaaaat gaaccgttaa tgcggccagt
    17281 taactcaaac tatgctcgtt attgcgccaa gc
//
LOCUS       DAZPEB010000076        17175 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZPEB010000076 DAZPEB010000000
VERSION     DAZPEB010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17175
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.077"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(64..345)
                     /locus_tag="ACHURJ_002190"
     CDS             complement(64..345)
                     /locus_tag="ACHURJ_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFR1281133.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQQHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            complement(608..1717)
                     /gene="apbC"
                     /locus_tag="ACHURJ_002191"
     CDS             complement(608..1717)
                     /gene="apbC"
                     /locus_tag="ACHURJ_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFR1281134.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1849..3882
                     /gene="metG"
                     /locus_tag="ACHURJ_002192"
     CDS             1849..3882
                     /gene="metG"
                     /locus_tag="ACHURJ_002192"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFR1281135.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPSNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMRQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            4023..7817
                     /locus_tag="ACHURJ_002193"
                     /pseudo
     CDS             4023..7817
                     /locus_tag="ACHURJ_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            7827..11459
                     /gene="yehI"
                     /locus_tag="ACHURJ_002194"
     CDS             7827..11459
                     /gene="yehI"
                     /locus_tag="ACHURJ_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein YehI"
                     /protein_id="HFR1281136.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRRWPGEPVSVITGSLIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSIPARTMAALACIAQ
                     NDSQQLLDEIVQQEGLEYATEVVIARQFIARCYESDPLVVTLQYQDEDYGYGYRSETY
                     NEFDLRLRKHLSLAEESCWQRCADKLIAALPGITKVRRPFIALILPEKPEIANELVGL
                     ECPRTHFHSKEWLKVVANDPTAVRKLEHYWSQDIFSDREASYMSHENHFGYAACAALL
                     REQGLAAIPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYHKNFP
                     HATLAALAELLALKEPPARPGNPIIEDKKLPAQQKARDEYWRTLLQTLMASQPQLAEE
                     VMQWLSTQPQSVLKSYLSAPPKPVIDGTDNSNLPEILVSPPWRSKKKMTAPRLDLAPL
                     ELTPQVYWQPGEQERLAATEPARYFSTESLAQRMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILNGWNSPEAQLAEQALRSGHIEALINIWEND
                     NYSRYRPEKSVWNLYLLAQLPREMALTFWLRINEKKHLFAGEDYFLSILGLDALPGLL
                     LAFSHRPKETFPLILNFGATELALPVARVWHRFAGQRNLARQWILQWPEHTATALIPL
                     VFVKPCDNSEAALFALRLLYEQGHSELLQTVANRWDRADMWPALEKILTQNPMEIYPA
                     RIPKAPDFWHPQMWSRPRLITNNQTVTNDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSAR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLETALRTTRRWSLADFHSLFVNHPFTRLVTQRLIWG
                     VYPANEPRCLLKAFRVAAEGEFCNAQDEPIDLPADALIGIAHPLEMTAEMRSEFAQLF
                     ADYEIMPPFRQLSRRTVLLTPDESTSNSLTRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYNLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVYRDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            11520..11837
                     /gene="yehK"
                     /locus_tag="ACHURJ_002195"
     CDS             11520..11837
                     /gene="yehK"
                     /locus_tag="ACHURJ_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YehK"
                     /protein_id="HFR1281137.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDADPLINDALIYIRDECIDERKLRGESPETVIAIFNRELQNIFNQ
                     EIE"
     gene            12144..13232
                     /locus_tag="ACHURJ_002196"
     CDS             12144..13232
                     /locus_tag="ACHURJ_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFR1281138.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDEAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVECIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            13243..15522
                     /locus_tag="ACHURJ_002197"
     CDS             13243..15522
                     /locus_tag="ACHURJ_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HFR1281139.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQDESQALLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDASGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQKIN
                     KPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQKAGEQLLKT
                     ILTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFTPDGLTQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLQEAARNKLEADMLDAGGIGSITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCATIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVNLPGVSLSAAVALLERRSQAIHAPALDRGAALGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     EADFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQNWGVFHV"
     gene            15515..16651
                     /locus_tag="ACHURJ_002198"
     CDS             15515..16651
                     /locus_tag="ACHURJ_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFR1281140.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVCQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIPSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            16648..>17175
                     /locus_tag="ACHURJ_002199"
     CDS             16648..>17175
                     /locus_tag="ACHURJ_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFR1281141.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANGQALKEAQCSCGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEH"
BASE COUNT         4315 a         4450 c         4371 g         4039 t
ORIGIN      
        1 agccgaaatc atttatataa atgatttcgg ctttctaaat gcaattaaaa tcaggttgcc
       61 aaattaatcg tagcgattta acacgattcg acgtttgcta tcgctacctg caacaaccac
      121 ttctgtcgtt acacctttga cgggcgaaga aaacagatga cgggtgtcaa cattatacgt
      181 taactgacca caatgctgtt gaggttgacc attaaccata gccatactgc atgttggggg
      241 actgatcctg ccattaatcg ctaatgtggc agtctctgaa gagaatgcgg gagaagccaa
      301 tccataggca agaaaaataa ttcctgacag ccaatactta ttcattgaac gttatccctg
      361 tagtaaaggt tatgcctgga tagaatgagt gcataacaaa ctatagctgt acatccacta
      421 cacagccacg aaggatgata atgaagcatt gcctgtatga tcaatcgact ttgtagagtt
      481 tcggacgaag gtccgcagaa tattcgcagt attaaataag tgttcattaa tgcggcaaaa
      541 actaatacac cgcatcaatg taacatctct gcggtgtatg aagaataacc agaatggtta
      601 ataggcgtta gaccgcgcgg aaggaaatct cgcctggaat gacttcaccc tgccagtaga
      661 gctgagctgc aacgcggtca gccagctggc ggtagatagc ggtaaattcg ctctccggac
      721 ggctgataac cgttggcgta cctttatcga gatcctcacg taaggagatg tgtaacggca
      781 tctgccccag cagctgtgta tggtattttt cagccagttt ctctgcacca ccggtaccga
      841 aaatcggttc atggtgacca cagttactgc aaatatgcac actcatgttc tcgacaatac
      901 cgagcaccgg aacctcgact ttctcgaaca tcacaatgcc ttttttcgca tcgatcagcg
      961 cgatgtcctg cggggtggtg accacgactg caccggttac tggaatgttt tgcgccagcg
     1021 tcaattgaat gtcaccggtg cctggcggca tatcaagcac cagatagtcg agatccggcc
     1081 acaaggtttc ctgcaacatc tgcattaacg ccttgctcgc catcggtcca cgccacacca
     1141 tcgcattgtc gtcagtgacc agataaccaa ttgaattggt tgccaggcca tgagacataa
     1201 tcggtgccat atgggtaccg tccggcgaag ttggacgctg attttctgcg cccaacattg
     1261 ttgggattga tgggccgtag atatcggcgt ccagaatgcc gactttcgcc ccttctgccg
     1321 ccagcgccag tgccaggttt accgccgtgg acgatttacc cacgccgcct ttaccggaac
     1381 tcaccgcgat aatgttcttc acaccgttaa ttcccggctg attcttcacg cgtttcagag
     1441 tggctatgtt atgcgacagt ttccagtcga tagctttcgc cccggtgatg cgcagcagtt
     1501 cggcgctaca ttgctctttt aactcttcga acgcactatg ccagacaaac ggcataacca
     1561 gttccacatg caacgtgtcg tccatccagg cgacatggtg caacgctttc agcgtggtga
     1621 ggttatgctt cagggttggg tgctgaaaat tggccagtgt cccggcgacc attgcgcgca
     1681 aggcttctgg tgatttggcc tgggattgtt cgttcatccc gactcctttt ttgtatagat
     1741 aaaccatcag ttgatagttt acctgaagaa tatagagaag tacttactta acattttccc
     1801 atttggtact atctaacccc ttttcactat taagaagtaa tgcctactat gactcaagtc
     1861 gcgaagaaaa ttctggtgac gtgcgcactg ccgtacgcta acggctcaat ccacctcggc
     1921 catatgctgg agcacatcca ggctgatgtc tgggtccgtt accagcgaat gcgcggccac
     1981 gaggtcaact tcatctgcgc cgacgatgcc cacggtacac cgatcatgct gaaagctcag
     2041 cagcttggta tcaccccgga gcagatgatt ggcgaaatga gtcaggagca tcagactgat
     2101 ttcgcaggct ttaacatcag ctatgacaac tatcactcga cgcacagcga agagaaccgc
     2161 cagttgtcag aacttatcta ctctcgcctg aaagaaaacg gttttattaa aaaccgcacc
     2221 atctctcagc tgtacgatcc ggaaaaaggc atgttcctgc cggaccgttt tgtgaaaggc
     2281 acctgcccga aatgtaaatc cccggatcaa tacggcgata actgcgaagt ctgcggcgcg
     2341 acctacagcc cgactgaact gatcgagccg aaatcggtgg tttctggcgc tacgccggta
     2401 atgcgtgatt ctgaacactt cttctttgat ctgccctctt tcagcgaaat gttgcaggca
     2461 tggacccgca gcggtgcgtt gcaggagcag gtggcaaata aaatgcagga gtggtttgaa
     2521 tctggcctgc aacagtggga tatctcccgc gacgcccctt acttcggttt tgaaattccg
     2581 aacgcgccgg gcaaatattt ctacgtctgg ctggacgcac cgattggcta catgggttct
     2641 ttcaagaatc tgtgcgacaa gcgcggcgac agcgtaagct tcgatgaata ctggaagaaa
     2701 gactccaccg ccgagctgta ccacttcatc ggtaaagata ttgtttactt ccacagcctg
     2761 ttctggcctg ccatgctgga aggcagcaac ttccgcaagc cgtccaacct gtttgttcat
     2821 ggctatgtga cggtgaacgg cgcaaagatg tccaagtctc gcggcacctt tattaaagcc
     2881 agcacctggc tgaatcattt tgacgcagac agcctgcgtt actactacac tgcgaaactc
     2941 tcttcgcgca ttgatgatat cgatctcaac ctggaagatt tcgttcagcg tgtgaatgcc
     3001 gatatcgtta acaaagtggt taacctggcc tcccgtaatg cgggctttat caacaagcgt
     3061 tttgacggcg tgctggcaag cgaactggct gacccgcagt tgtacaaaac cttcactgat
     3121 gccgctgaag tgattggtga agcgtgggaa agccgtgaat ttggtaaagc cgtgcgcgaa
     3181 atcatggcgc tggctgatct ggctaaccgc tatgtcgatg aacaggctcc gtgggtggtg
     3241 gcgaaacagg aaggccgcga tgccgacctg caggcaattt gctcaatggg catcaacctg
     3301 ttccgcgtgc tgatgactta cctgaagccg gtactgccga aactgaccga gcgtgcagaa
     3361 gcattcctca atacggaact gacctgggat ggtatccagc aaccgctgct gggccacaaa
     3421 gtgaatccgt tcaaggcgct gtataaccgc atcgatatga ggcaggttga agcactggtg
     3481 gaagcctcta aagaagaagt aaaagccgct gccgcgccgg taactggccc gctggcagat
     3541 gatccgattc aggaaaccat cacctttgac gacttcgcta aagttgacct gcgcgtggcg
     3601 ctgattgaaa acgcagagtt tgttgaaggt tctgacaaac tgctgcgcct gacgctggat
     3661 ctcggcggtg aaaaacgcaa tgtcttctcc ggtattcgtt ctgcttaccc ggatccgcag
     3721 gcactgattg gtcgtcacac cattatggtg gctaacctgg caccacgtaa aatgcgcttc
     3781 ggtatctctg aaggcatggt gatggctgcc ggtcctggcg ggaaagatat tttcctgcta
     3841 agcccggatg ccggtgctaa accgggtcat caggtgaaat aatccccctt caaggcgctg
     3901 catcgacagc gccttttctt tataaattcc taaagttgtt ttcttgcgat tttgtctctc
     3961 tctaacccgc ataaatactg gtagcatctg cattcaactg gataaaatta cagggatgca
     4021 gaatgagaca ctttatctat caggacgaaa aatcacataa attcagggca gttgagcaac
     4081 agggaaacga gttgcatatc agttggggaa aagttggcac caaagggcaa agccagataa
     4141 aaagtttttc agatgctgcg gcagcggcaa aagcggagct taagctgatt gcggagaagg
     4201 tgaagaaggg gtatgtggag caagcgaagg ataattcttt gcaaccttcc caaacggtaa
     4261 cgggctctct caaggtagcg gacttatcca ccattattca ggaacaaccc tctttcgtag
     4321 cagaaacccg tgcgcctgac aaaaatacag atgctgtttt accgtggctg gcgaaagata
     4381 ttgctgtcgt ttttccgccc gaagttgtac acaccacgtt aagtcatcgc cgctttcccg
     4441 gagttcctgt tcagcaagca gacaaattgc cccaattacg tcgcttagcc tgtagtgtgt
     4501 cgcaacggga taataaaaca gccacatttg acttcagcgc ctgttcttta gaatggcaaa
     4561 acaccgtcgc ccaggcgatc agtcagatcg acggcctgaa aacaacacag ttaccatcac
     4621 cagtaatggc tgtactcacg gcacttgaaa tgaaatgcac aagatataaa gtgcgtgagg
     4681 atgttatgga tcagatcgtc caggaaggcg gtctggaata tgctactgat gtaataatac
     4741 accttcaaca gattgatatt gaatgggatt atgcgaataa tgtcattatt attctgccgt
     4801 ctggcattgc acctagctac ttggagcaat attccagatt tgaatgacgc ctacgtaaac
     4861 atttatcact gacggaagag tctctctggc aaaaatgtgc acaaaaactt attgccgcaa
     4921 ttccacatat tccagaatgg cggcaaccat taattgcttt gttattaccc gaaaaaccag
     4981 aaattgcaca tgaaattgcc cagcgtctac tggggcaaaa aaaattaccc tcgcttgagt
     5041 ggttaaaaat agtggcgact gatgagcaca ttcttgcctc attagaaaaa tatcacgaac
     5101 catatgccat ttttgatgat tactattgtg gtgcgatatg gtcagccacc gtattacagg
     5161 agcaaggtgt tgcagccctg ccccgatttg ctccctatgc cgcaagtgac tactgcgccg
     5221 atgtgttgcg tcatatcaat catccgttcg cattgacact gcttatacgt gtagccgggc
     5281 aaactaaacg ctgtcacgat cggatgacga aagccattgc tgcgttccca catgcagcaa
     5341 tggcggcact gacggaactt cttgggcaaa aagaagagaa cagttggcgc attatgctaa
     5401 tgacaatgct tatctcacaa ccagcactgg cagaacaggt cattccctgg ctctcgacac
     5461 ccgcagttgc cgtactgaaa tcatgccagc aacaactgac acagccctca aaccatgcca
     5521 gcgccgatct actgccagcc gtagtagtct cccctccctg gctttcgaaa aagaaaaaat
     5581 cgccgattcc ggtgctggat ttagcgccat taggcattga gccaatctgt tatctgacag
     5641 aagaaatcag taatcaactt ttggcgaaat atatctggta ttcaaaacac atcacggtta
     5701 gccatgaaga aagtactacc aacctgttgg caaggatggg ttttcaacga cggatcgctg
     5761 gtacatatat taaagctccc gaagcggtag ttgaggcatg gctaaatgaa gattattcaa
     5821 ccttactaag tgaatttaag gtgtttcatt cacctaccgg gcattattgg cagttgggga
     5881 ttttgacaac attgccgctg gagaaagcag taaaagcatg gaatgccctt accctatctc
     5941 cacataccga taccgaatac tccatgttac attttggact caaagggtta cctgggttag
     6001 taaactcact tgcacgctat ccacaagaag ccttgcccat cacgaattac ttcgcagcga
     6061 gtgagctggc tcctgccgtc gcccgtgcct tcaacaaact gaaaacgcta cgcgaaaacg
     6121 cccgtagctg gctgttgaaa tacccggaac atgcccttac cggcctgctg cctgcggcgc
     6181 tcggcaaagc cggtgaagca caggataacg cccgcgctgc cttgcgtatg cttaccgaaa
     6241 acggtcatca gccattactg caagaaatcg cccgacgtta taaccagccg gaagtaaccg
     6301 atgcggtgaa cgctctgctt gcgctcgatc ccttagataa tcacccgaca aaaatcccca
     6361 ctcttccggc cttttatcag ccatcgctct ggacgcgccc ggtattaaaa gcaaatgccc
     6421 aatcactgcc agatagcgcc ctcctccacc tcggtgaaat gctccgcttc cctcaggaag
     6481 aggctctgta tccgggatta ttgcaggtga aagacgtctg ttccgccgac tcactggcgg
     6541 gatttgcctg ggatctgttt accgcctggc agaccgctgg cgcgccgtcg aaagagagtt
     6601 gggcgttcac tgcgttaggc gttctcggta acgatgacac cgcccgcaaa ctgacgccat
     6661 taatacgcgc ctggcctggt gaatcccagc ataaacgcgc caccgttggg ttggatattc
     6721 tcgctgctat cggtagtgat atcgccctta tgcagcttaa cggcatcgcc cagaaactga
     6781 aattcaaagc attacaggag cgggcaaaag aaaaaattgc cgacattgcc gagagccgcg
     6841 aactcacggt ggcggagctt gaagatcggt tagcaccgga tctcggtctg gatgataacg
     6901 gttcgctgct gctggatttt ggcccacggc agttcaccgt cagctttgat gaaaccttaa
     6961 aaccgtttgt gcgtgatgtt tccggcagcc gcctgaaaga cctgcccaaa ccgaacaaaa
     7021 gcgatgatga aacgcgggcg aacgatgcgg ttaaccgcta caaattgctg aaaaaagatg
     7081 cgcgtaccat cgccgcccag caggtagcaa ggctggaatc cgccatgtgc ctgcgccgcc
     7141 gctggtcgct ggaaaacttc cagctcttcc tggttgagca tccgctggtt cgtcacttaa
     7201 cccgccgtct gatttggggc gtttatagcg ccgaaaacca gctactggct tgctttcgcg
     7261 tagcagaaga taacagctcc agcaccgctg acgatgatct tttcaccctg ccggaaggcg
     7321 atatctctat cggcactcct cacgttctgg aaatatcacc aacggatgct gccgcctttg
     7381 gtcagctttt tgccgactac gaactgctac caccgttccg ccagctcgac cgtaacagct
     7441 acgccctgac agaagccgag cgcaatgcca gtgaactgac ccgctgggca ggcagaaaat
     7501 gcccgagtgg tcgggtcatg gggctggcga ataaaggctg gataaagggc gaaccacagg
     7561 atggaggctg gatcggatgg atgatcaaac ctttgggtcg ctggtcgtta atcatggaaa
     7621 tcgatgaagg ctttgcggta ggcatgtcgc cagccgaact cagcgctgag cagctcttaa
     7681 gcaagctgtg gctatgggaa ggcaaagcag aaagatatgg ctgggggagt aattcaacac
     7741 aggaagcgca gttctccgta atcgatgcca tcaccgccag cgagctaatt aacgatattg
     7801 aagccctgtt tgaataagga aagagcatgg acaaggaatt accgtggctg gcggataacg
     7861 cccaactgga actgaaatat aaaaaaggca aaacgccgct cagtcatcgt cgctggccgg
     7921 gcgaaccagt gtccgttatc actggaagtc tcatccagac attgggtgat gaattgctac
     7981 aaaaagctga gaagaaaaaa aacattgtct ggcgttatga gaatttttca ctggagtggc
     8041 agtccgccat cacgcaggcc atcaacttga tcggcgaaca caaaccctca atcccggccc
     8101 ggacaatggc ggcgctagcc tgtatcgcgc aaaatgacag ccaacagttg ctcgacgaaa
     8161 tcgtccaaca agaggggctg gaatatgcga ctgaggtggt gattgcacgc cagtttattg
     8221 cgcggtgtta tgagagtgat cctctggtag tgacattgca gtatcaggac gaggattatg
     8281 gctatggtta tcgctcagaa acctataacg aattcgatct ccgactgcgt aagcatctct
     8341 ctctggcaga ggaaagctgc tggcagcgtt gcgccgacaa actcattgcc gcactaccag
     8401 gaataaccaa agttcgccgc ccttttattg cgctgatcct cccggaaaaa ccagaaatag
     8461 ccaatgagtt ggtaggcctt gaatgcccgc gaactcattt tcattctaag gagtggttaa
     8521 aagttgttgc taatgacccc acagcggtga gaaaactcga acactactgg agccaggata
     8581 tatttagcga tcgagaagcc agctacatgt cgcatgaaaa ccacttcggc tacgcggcct
     8641 gcgccgccct tttgcgcgaa caaggactgg cagccattcc gcgcctcgcg atgtatgccc
     8701 ataaagaaga ttgcggcagt ctgctggtac aaattaacca tccgcaagtc atccgcacct
     8761 tgctactggt ggctgataaa aacaaaccca gcctgcaacg tgtagctaaa taccataaaa
     8821 acttccccca tgcgacgctc gccgcactgg cagaactgct ggcgttaaaa gaaccaccag
     8881 cccgcccagg taatccaatc atcgaagaca aaaagctgcc tgcacagcaa aaagcacgcg
     8941 atgaatactg gcgtacgctg ttacaaacgc tgatggcatc gcagccacaa ctggcagaag
     9001 aggtgatgca gtggttaagt actcaacccc agtcagtgct gaagagttat ttatcggcac
     9061 cgcccaaacc ggttattgat ggcaccgata acagcaatct gccagaaatc ctcgtttcac
     9121 caccgtggcg tagtaagaaa aaaatgacag ctccacgtct tgatttggca ccgctcgaat
     9181 taactccgca agtttactgg caaccaggcg aacaagagag gcttgccgcc actgagcctg
     9241 cccgttattt cagcacggaa tctcttgcgc aacgcatgga acaaaaaagt ggacgagttg
     9301 tattacagga actgggtttt ggggatgatg tatggctgtt tctgaattat atactccccg
     9361 gaaaactgga tgctgcacgc aattcactca ttgttcagtg gcattactac caggggcggg
     9421 ttgaagagat cctgaatggc tggaactccc cggaagcaca attagcagaa caggcgctcc
     9481 gcagcggtca catagaagcg ttaattaaca tatgggaaaa tgacaactac tcacgttatc
     9541 gtccggaaaa gagtgtctgg aacctgtatt tattggcaca gttgccgcgt gagatggctt
     9601 tgaccttctg gctgcgtatc aatgagaaaa agcatctgtt cgcgggtgag gactattttc
     9661 tcagtatcct cggattggat gcgctaccag gtctgctgtt ggctttttca catcgtccaa
     9721 aagaaacatt tccgttaatt ttaaatttcg gcgcaacaga actggccctg cccgttgccc
     9781 gcgtctggca ccgttttgcg ggccagcgta atctggctcg ccagtggatt ttacaatggc
     9841 cggaacatac ggctactgca cttattccac tcgtctttgt taaaccctgc gacaacagcg
     9901 aagcggcatt atttgccctt cgtttactgt atgaacaagg acatagtgaa ttactgcaaa
     9961 cggttgcaaa ccgctgggat cgcgctgata tgtggccagc cctggaaaaa atacttaccc
    10021 agaacccgat ggaaatttac ccggcacgca ttccaaaagc ccctgatttc tggcatccgc
    10081 aaatgtggtc caggccgcgc cttatcacta ataatcaaac tgttaccaat gacgctctgg
    10141 aaattatcgg cgaaatgctg cgctttaccc aggggggacg tttttatagc gggctggaac
    10201 aactgaaaac gttctgccag ccacaaacgc tggcagcttt tgcttgggat ctcttcactg
    10261 cgtggcaaca agctggtgcc cccgcaaaag acaactgggc atttctggcg ttaagtctct
    10321 ttggtgacga aagcacggca cgggatctga cgacacagat cctcgcctgg ccacaagaag
    10381 gcaaatctgc ccgtgctgtc agcggcctga acatccttac cctgatgaat aatgatatgg
    10441 cgctgataca gctgcatcat atatcgcaac gggctaaatc ccgcccctta cgtgataacg
    10501 cggcggaatt tcttcaggtg gtcgcagaaa atcgcgggct aagccaggaa gagctagcgg
    10561 acagattagt cccaaccctg ggccttgatg atccgcaggc gttgagtttt gattttggtc
    10621 cccggcagtt taccgttcgc ttcgatgaaa atctcaaccc ggttatcttt gatcagcaaa
    10681 acgttcgcca gaaaagcgtt ccccgtttgc gcgccgatga cgatcaactg aaagcgcccg
    10741 aggcactggc ccgactaaaa gggctaaaaa aagatgctac tcaggtgagc aaaaacctgc
    10801 tcccgcgtct tgaaacggcc ctacgtacca cccgacgctg gtcgctggca gattttcatt
    10861 ctctgtttgt taatcatccc tttacccgtc tggttaccca gcgattaata tggggggttt
    10921 atccggcaaa tgaaccgcgt tgtttactca aagcctttcg tgtggccgca gagggggagt
    10981 tctgcaatgc gcaagatgag ccaattgacc tgcctgcgga cgctctgatt ggcattgccc
    11041 acccgttaga aatgacagca gaaatgcgca gtgaatttgc acagcttttt gccgattacg
    11101 aaattatgcc gccttttcgc cagttgtcgc gccgcacggt gctgctcaca cctgacgagt
    11161 caaccagtaa cagcctgact cgctgggaag gtaaatccgc taccgttggg caacttatgg
    11221 gaatgcgata caaaggctgg gagtcaggct atgaggacgc atttgtctat aacctgggtg
    11281 agtaccggct ggtccttaag ttttcacccg gttttaacca ctacaatgtt gatagcaaag
    11341 cgctaatgag cttccgttct cttcgagtgt accgtgacaa taaatccgtc acttttgccg
    11401 aacttgatgt gtttgatttg agtgaggcgt taagcgcacc tgacgtcatt ttccattaaa
    11461 acacagcggg cagtgatgca actgcccgtt atcaacacga cttaccagcg gggaaagcga
    11521 tgatcgtgca aaaagagctg gttgctattt acgattatga ggtccctgta cctgaagatc
    11581 cgttttcctt cagacttgag atccataaat gctctgaatt atttacaggt tccgtctatc
    11641 gactggagcg attccggcta cgtccaacat ttcatcaacg tgatcgagaa gatgctgacc
    11701 cgctaataaa tgatgcgttg atttatataa gagatgagtg tattgatgag cggaaattac
    11761 gaggtgaatc acctgaaact gtaatagcaa tttttaatcg tgaactacag aatatattca
    11821 accaagaaat agaataatat actctaaata attcaaattg gtccgatccg gcgcaacgtc
    11881 ccaatggcct ggattataaa tctcattatc ttaattgcaa cggggtccag ccgtggttat
    11941 aattcgttcg ttggaacacg atgttgcaca ggctgtggtg ttaggcctga aaatagtaaa
    12001 taaaagaacc tgcctcacca gcaggttttt ttatttactg tgatctgctt tccagatatt
    12061 tttcgctcaa acaactaatg cgccaaacat ttattgcgcg taaaatatcg tttatttcat
    12121 taatacattt cagggatgaa tatatgtcac cgcagaataa tcatctgcag cgtccgcctg
    12181 ctgctgtgtt atacgccgat gaactggcaa aattaaaaca aaatgataac gcaccttgcc
    12241 cgcccggttg gcagttaagt ttgcctgcgg cccgtgcttt tatccttggc gacgaagcgc
    12301 aaaatatcag ccgtaaagtg gtgattagcc cctccgctgt cgaacgtatg ttagtcactc
    12361 ttgctaccgg ccgcggtttg atgttggtgg gggaaccggg taccgcaaaa tctcttcttt
    12421 ctgaattact ggcaaccgct attagcggcg atgccgggtt aaccatccag ggcggggcat
    12481 ctaccaccga agatcaaatc aagtatggct ggaactacgc cctgcttatc aatcatggtc
    12541 cgtcaacgga agcgctggtc cctgccccgc tttatcaggg aatgcgcgac ggcaaaatcg
    12601 tccgtttcga agagataacg cgtacaccgc tggaagtgca ggactgtctg ttaggtatgc
    12661 tttctgacag ggtgatgacg gtgccggaac tcactggtga agccagtcag ctctatgcgc
    12721 gagaaggctt taatattatt gccactgcca atacccgcga tcgcggcgtc aatgaaatga
    12781 gtgctgcgct aaagcgtcgc ttcgattttg aaacggtttt tccgattatg gatttcgccc
    12841 aggagttgga actggtcgcc agtgcttcgg cgcgtttgct ggcccatagc ggtattccgc
    12901 ataaagtgcc tgacgcggta ctggaattac tggtccgcac cttccgcgat ctgcgtgcca
    12961 acggcgaaaa gaaaacgtca atggatacac tgacagcgat tatgtccacc gctgaagccg
    13021 tgaacgtggc ccatgctgtg ggcgtccgcg cctggttttt agcgaatcgc gcgggggagc
    13081 ctgccgacct ggtggaatgt attgccggaa ccatcgtcaa agataacgaa gaagatcgcg
    13141 cgcgtctgcg ccgttacttc gaacagcgtg tcgccacaca taaagaagct cactggcagg
    13201 cttattatca agcccgccac cgcctgccgt gaggaaagat gcatgagcga gccgttaatt
    13261 gtcggcatcc ggcatcatag tccggcctgc gcccggctgg tgaaatcgtt aatcgaaagc
    13321 cagcggccac gatacgtgtt gattgaaggc ccggctgatt ttaatgaccg ggtagacgaa
    13381 ctgtttttag cccaccagct tccggtagct atttacagtt attgccagta tcaggacggt
    13441 gcagcccccg ggcgtggtgc ctggacgcca tttgctgaat tttcgccgga gtggcaggcg
    13501 ctacaagccg cacgtcgcat tcaggcacaa acttacttca tcgatttgcc ttgctgggcg
    13561 cagagtgaag aagaggacga ttcgcctgat acgcaagatg aaagccaggc cttactgctg
    13621 cgtgccaccc gcatggataa cagcgatacc ctgtgggatc acttgttcga agatgaaagc
    13681 cagcaaactg cattaccctc tgcgctggcg cactattttg cccaactgcg gggcgacgcc
    13741 tccggcgatg cgctcaatcg tcagcgcgaa gcctttatgg cccgctggat tggatgggcg
    13801 atgcagcaaa ataatggcga cgtgttagtt gtctgcggtg gctggcacgc tccggcactg
    13861 gcaaagatgt ggcgcgaatg ccctcagaaa attaacaagc cagaattgcc ctcgctggca
    13921 gatgccgtta caggttgtta tctcacaccc tacagtgaaa agcgccttga tgtgctggca
    13981 ggataccttt caggaatgcc tgccccggta tggcaaaact ggtgctggca gtggggcttg
    14041 cagaaggccg gtgaacaact gctaaaaact atccttaccc gtttgcgcca gcacaaattg
    14101 cccgcttcta ccgcggatat ggctgccgct catctgcatg cgatggcgct ggcacagttg
    14161 cgcggtcata cactaccgtt acgcactgac tggctggatg ccatagcagg ctcgctgatt
    14221 aaagaagccc tgaacgcgcc gttgccgtgg agctatcgcg gcgttattca tcccgatacc
    14281 gatccgattc tgctaacgtt gatagacaca ttagcgggtg acggattcgg taaacttgcc
    14341 ccttctacgc cacaaccgcc tctgccaaaa gatgtcacct gcgaactgga acgtaccgca
    14401 atctcccttc cggcggagct taccttaaat cgctttaccc ccgatgggct gacgcaaagt
    14461 caggtgttac atcggctggc aatactggag atccctggga ttgtacgcca gcagggaagt
    14521 acactgacac ttgcaggcaa cggtgaagaa cgctggaaat taacccgccc gcttagccag
    14581 catgcggcat tgattgaggc cgcctgtttt ggtgccacac tccaggaagc cgcacgcaat
    14641 aaattagaag ccgatatgct ggacgcgggc ggaatcggca gtatcaccac atgtcttagc
    14701 caggcggcgt tagcgggtct ggcgtccttc agtcaacaat tactggagca actcacacta
    14761 ttaatcgccc aggaaaatca atttgccgaa atgggccagg cgctggaagt gctttatgcc
    14821 ttatggcggc tggatgaaat tagcggtatg caaggcgcgc agatattaca aacgacgtta
    14881 tgcgcgacta tcgatcgcac gctgtggctg tgtgaatcta acggcagacc ggatgaaaag
    14941 gagtttcacg ctcacctgca tagctggcaa gcgctttgcc atattctgcg cgatctacat
    15001 agcggcgtta atttacccgg cgtttctctt tctgcggcgg tagccttact ggagcgacgc
    15061 agtcaggcaa ttcatgcccc ggcgctggat cgcggcgcgg ctcttggcgc actaatgcgt
    15121 ctggaacatc ccaacgccag tgccgaagcg gcgctgacga tgctggcgca gttatccccg
    15181 gcacaatctg gtgaggcgct gcacggtttg ctggcgctgg cccgccatca actggcctgt
    15241 cagccggcat ttatcgccgg tttcagcagt catttaaatc aactgagtga agccgatttt
    15301 attaacgccc tgcccgattt acgcgcggcg atggcctggc taccaccacg agaacgcggg
    15361 acgctggcgc atcaggtgct tgagcattat caactggcgc aacttcccgt ttcggcgctg
    15421 caaatgccgt tgcattgtcc accacaggcc attgcacatc atcaacaact cgaacagcag
    15481 gcactggcat cgctgcaaaa ctggggagtt ttccatgtct gaactgaacg atcttctgac
    15541 cacccgtgag ctacaacgct ggcgattaat tcttggcgaa gcggcagaaa cgacgctttg
    15601 tgggctggat gacaacgccc ggcagataga ccacgcgctg gagtggctgt atgggcgcga
    15661 tcctgaacgg ctccagcgtg gtgaacgttc cggtggatta ggtggctcaa atctcaccac
    15721 ccctgagtgg atcaacagta ttcacacgct gtttccgcag caggtgattg agcggctgga
    15781 aagcgatgcc gtgctgcgct acggcattga agatgtggtg acgaatctcg acgtgctgga
    15841 acgtatgcag ccttctgaaa gcctgctacg cgctgttttg cacaccaaac atctgatgaa
    15901 ccccgaagta ctggctgccg cccgccggat agtgtgccag gttgttgaag aaattatggc
    15961 tcgactggca aaggaagttc gtcaggcttt ttctggtgtc cgcgatcgcc gtcgccgttc
    16021 atttattcca ctggcgcgaa actttgattt caaaagtact ctgcgcgcca acctgcaaca
    16081 ctggcacccg caacacggca agttgtatat cgaatccccc cgctttaaca gccgcattaa
    16141 acgccaaagc gaacaatggc aactggtctt actggttgat caaagcggct cgatggtcga
    16201 ttcggtgatc ccctctgcgg tgatggcggc ctgtttgtgg cagttacccg gcattcgtac
    16261 ccatctggtg gcgtttgaca caagcgtcgt tgatctcacg gcagacgttg ccgatccggt
    16321 agagttatta atgaaagtac agttgggcgg cgggaccaat atcgccagtg ccgtggagta
    16381 tggtcggcaa cttattgaac aaccagcgaa aagcgtcatt atcctcgtga gcgattttta
    16441 cgaagggggt tcatcatcat tactgacgca tcaggtgaaa aagtgtgtcc agagcggcat
    16501 caaagtgctg ggactggcag cgctcgatag cacagcaaca ccttgctatg accgcgatac
    16561 ggcccaggcg ctggttaatg tcggcgcaca aatagccgcc atgacaccgg gcgagctggc
    16621 atcatggctt gcggagaatc ttcagtcatg aattcactac gtccggaatt attagaactg
    16681 acaccgcagg ccctgacggc gttaagcaat gccggttttg ttaagcgcag tcttaaggaa
    16741 ctggaaaatg gcaacgtccc ggagatcagc catgagaacg gcgctttaat cgccaccttc
    16801 agtgacggtg tccgtaccca gctggcgaac ggccaggcac tgaaagaggc tcagtgcagc
    16861 tgcggggcca gcggtatgtg ccgtcatcgc gtgatgctgg tgttaagtta tcaacgactt
    16921 tgtgctaccg cccagcctac gggaaaagaa gaggaatggg atccggcaat ctggctggaa
    16981 gaactggcta cccttcccga tgccacccgc aaacgcgcac aggcgctggt cgctaaaggc
    17041 atcaccattg agttgttctg tgcgccgggt gaaattccct ctgcccgctt accgatgagc
    17101 gatgtgcgtt tttattcccg cagcagtatt cgtttcgccc gttgtgattg tattgaaggc
    17161 acactttgcg aacat
//
LOCUS       DAZPEB010000077        16798 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZPEB010000077 DAZPEB010000000
VERSION     DAZPEB010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16798
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.078"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..2018
                     /gene="fhuE"
                     /locus_tag="ACHURJ_002200"
     CDS             <1..2018
                     /gene="fhuE"
                     /locus_tag="ACHURJ_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HFR1281142.1"
                     /translation="DYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLEISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAIDMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSGWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNMEGSIVYGAPRNFSITGTYQF"
     gene            complement(2078..3511)
                     /gene="ptsG"
                     /locus_tag="ACHURJ_002201"
     CDS             complement(2078..3511)
                     /gene="ptsG"
                     /locus_tag="ACHURJ_002201"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFR1281143.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMHDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(3806..4603)
                     /locus_tag="ACHURJ_002202"
     CDS             complement(3806..4603)
                     /locus_tag="ACHURJ_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR1281144.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(4614..5618)
                     /gene="holB"
                     /locus_tag="ACHURJ_002203"
     CDS             complement(4614..5618)
                     /gene="holB"
                     /locus_tag="ACHURJ_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFR1281145.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYTVTWLSREVTMSQDALLAALRLSAGSPSAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(5615..6256)
                     /gene="tmk"
                     /locus_tag="ACHURJ_002204"
     CDS             complement(5615..6256)
                     /gene="tmk"
                     /locus_tag="ACHURJ_002204"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR1281146.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(6246..7268)
                     /gene="yceG"
                     /locus_tag="ACHURJ_002205"
     CDS             complement(6246..7268)
                     /gene="yceG"
                     /locus_tag="ACHURJ_002205"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFR1281147.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(7271..8080)
                     /gene="pabC"
                     /locus_tag="ACHURJ_002206"
     CDS             complement(7271..8080)
                     /gene="pabC"
                     /locus_tag="ACHURJ_002206"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFR1281148.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(8200..9441)
                     /gene="fabF"
                     /locus_tag="ACHURJ_002207"
     CDS             complement(8200..9441)
                     /gene="fabF"
                     /locus_tag="ACHURJ_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFR1281149.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKAIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNLDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(9529..9765)
                     /gene="acpP"
                     /locus_tag="ACHURJ_002208"
     CDS             complement(9529..9765)
                     /gene="acpP"
                     /locus_tag="ACHURJ_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFR1281150.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(9976..10710)
                     /gene="fabG"
                     /locus_tag="ACHURJ_002209"
     CDS             complement(9976..10710)
                     /gene="fabG"
                     /locus_tag="ACHURJ_002209"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFR1281151.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(10723..11652)
                     /gene="fabD"
                     /locus_tag="ACHURJ_002210"
     CDS             complement(10723..11652)
                     /gene="fabD"
                     /locus_tag="ACHURJ_002210"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFR1281152.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(11668..12621)
                     /gene="fabH"
                     /locus_tag="ACHURJ_002211"
     CDS             complement(11668..12621)
                     /gene="fabH"
                     /locus_tag="ACHURJ_002211"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFR1281153.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVANQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(12689..13759)
                     /gene="plsX"
                     /locus_tag="ACHURJ_002212"
     CDS             complement(12689..13759)
                     /gene="plsX"
                     /locus_tag="ACHURJ_002212"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFR1281154.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLRPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(13840..14013)
                     /gene="rpmF"
                     /locus_tag="ACHURJ_002213"
     CDS             complement(13840..14013)
                     /gene="rpmF"
                     /locus_tag="ACHURJ_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFR1281155.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(14065..14586)
                     /gene="yceD"
                     /locus_tag="ACHURJ_002214"
     CDS             complement(14065..14586)
                     /gene="yceD"
                     /locus_tag="ACHURJ_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFR1281156.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            14785..15369
                     /gene="yceF"
                     /locus_tag="ACHURJ_002215"
     CDS             14785..15369
                     /gene="yceF"
                     /locus_tag="ACHURJ_002215"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFR1281157.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLCEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(15481..16440)
                     /gene="rluC"
                     /locus_tag="ACHURJ_002216"
     CDS             complement(15481..16440)
                     /gene="rluC"
                     /locus_tag="ACHURJ_002216"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFR1281158.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRSQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
BASE COUNT         4179 a         4559 c         4052 g         4008 t
ORIGIN      
        1 atgattacag cgtaacgtct acctctgcgg gtaccaaaat gcagatgact caacgtgata
       61 ttcctcagtc ggtcactatt gttagccagc agcggatgga agatcagcag ttacaaacgc
      121 tgggcgaagt gatggaaaac acgctggaga tcagcaaaag tcaggcggat tccgatcgtg
      181 ctctttatta ttcccgcgga ttccagatcg ataactatat ggttgatggt atccccacct
      241 attttgaatc gcgctggaat ctgggcgatg cactttctga tatggcactg tttgaacgcg
      301 tagaagtagt gcgtggcgcg acaggactca tgaccgggac gggtaatcca tctgcggcaa
      361 ttgatatggt tcgaaaacac gcgaccagtc gtgaatttaa aggcgatgtc tcggcggaat
      421 acggtagctg gaacaaagaa cggtatgtgg cggatttaca aagcccactc accgaagacg
      481 gtaaaatccg cgcgcgaatt gtcggcggct accagaataa cgactcatgg ctggaccgct
      541 acaacagtga aaagaccttc ttctccggca ttgtcgatgc tgatttaggc gatcttacga
      601 cgctttcagc cggttacgaa tatcagcgca ttgatgttaa tagccctacc tggggcggtt
      661 taccgcgctg gaatactgat ggcagcagca acagttacga tcgtgcacgc agtaccgcac
      721 ctgactgggc gtacaacgat aaagagatca acaaggtctt tatgaccctg aagcagcggt
      781 ttgctgatac ctggcaagcg acactgaatg ccacccactc tgaagtcgaa tttgacagca
      841 aaatgatgta tgtcgatgcc tatgtaaaca aagcggatgg tatgctggtt gggccataca
      901 gtaattatgg acctggcttt gattatgtcg gcggcaccgg ttggaacagt ggcaaacgta
      961 aagttgatgc gctagatttg ttcgctgacg gtagttatga attgtttggt cgtcagcaca
     1021 atctaatgtt tggtggcagt tacagcaaac aaaacaatcg ttacttcagt tcatgggcca
     1081 acatcttccc ggatgaaatt ggcagtttct acaactttaa tggcaatttc ccacaaaccg
     1141 actggtcacc acagagcctg gcacaggacg ataccacaca tatgaaatcg ttatatgctg
     1201 ccactcgagt cacccttgcc gatccgctgc atctgatcct cggcgcacgc tataccaact
     1261 ggcgggttga tacgctgact tacagcatgg agaaaaacca caccacgcct tacgctggtc
     1321 tggtgtttga catcaatgac aactggtcga cctacgccag ctatacctct attttccagc
     1381 cgcaaaatga tcgtgacagt tcaggcaaat atctggctcc aatcaccggt aacaactacg
     1441 agctgggtct gaaatcgggc tggatgaata gccgtctgac caccacgtta gccatcttcc
     1501 gtattgagca ggataatgtc gctcagtcca ccggtacacc tatccccggc agcaacggcg
     1561 aaaccgccta taaagcggtg gatgggacag tcagtaaagg ggtggaattt gaactcaacg
     1621 gcgcaattac cgacaactgg cagctgacat ttggcgcaac gcgctatatt gcagaggata
     1681 acgaaggaaa cgccgttaat cctaatctgc cacgcaccac ggttaaaatg ttcaccagct
     1741 atcggttgcc tgtcatgcca gagttgacag tcggcggtgg tgtgaactgg caaaatcgcg
     1801 tgtataccga caccgtgaca ccgtatggca ccttccgcgc cgagcaaggt agctacgcgc
     1861 tggtggatct cttcacccgc taccaggtga cgaaaaactt ctcgttacag gggaacgtca
     1921 ataacctgtt cgacaaaacc tacgatacca acatggaagg ttctatcgtc tacggcgcac
     1981 cgcgtaattt cagcattacc ggcacgtatc aattctgaat aacacctgta aaaaaggcag
     2041 ccatctggct gccttagtct ccccaacgtc ttacggatta gtggttacgg atgtactcat
     2101 ccatctcggt tttcaggtta tcggatttag taccgaaaat cgcctgaaca ccagaacccg
     2161 caaccactac gcccgctgcg cccagtttct tcaggccagc ctgatccact ttagacacat
     2221 cagcaacgct gacgcgcaga cgggtaatac atgcgtcgag gttagtaatg ttttctttac
     2281 caccaaatgc agcaaccaga gccggtgcca tttcgctggt acctgttgct tttgcatctt
     2341 cagtcgcgtc ttcacgaccc ggcgttttca gatccagtgc tttaatcagc acgcggaaga
     2401 tggtgtagta aacaatcgca taaccgatac cgacgatcgg gaacagccac agtttgctgc
     2461 tgttaccaga cagaacgata aagtcgatca gaccgtgcga gaacgacgta ccgtcatgca
     2521 tccccagaag aatacagatt gggaatgcca ggcctgccag aatcgcgtgg atgatgtaca
     2581 ggatcggcgc aacgaacatg aaggagaact cgatcggctc ggtgataccg gtcaggaacg
     2641 aggtcagcgc cgcggagatc ataataccgc ccactttcgc gcggttttct ggtttagcag
     2701 agtgccagat agcaattgcg gcagctggca gaccgtacat tttgaacagg aagccaccag
     2761 acagtttacc cgcagtcggg tcacccgcca tataacgcgg aatgtcgccg tggaaaacct
     2821 gacctgctgc gttggtgtat tcaccaatct gcatctggaa aggtacgttc cagatgtggt
     2881 gcagaccaaa cggtaccagg caacgttcga tgaaaccgta aatgccaaac gcaactaccg
     2941 ggttctggta agcagcccac tgagagaagg tctggattgc agaaccaatc ggcggccaaa
     3001 tgaaggacag cacaacgcca gtaaagatgg cagccaggcc agaaatgatc ggcacaaagc
     3061 gtttaccggc aaagaagcca agatactcag gcagcttaat acggtagaaa cggttaaaca
     3121 tgtacgctgc gatcgcaccg gagataatcc ctccgagtac gccagtatcc gccaggtgtt
     3181 tagaggcgat ttcttcagca ggtaaatgca gtaccagtgg cgcaaccacg gccatggttt
     3241 taaccatgat gccataggca acaactgcgg ccagcgcgga tacgccatcg ttattggtaa
     3301 agccgagggc gacaccgatc gcaaaaatca gtggcatgtt tgcaaagacg gaaccgcctg
     3361 cttctgccat aacatgcgat acaacggcgg gcagccagct gaaattcgcg gaaccgacgc
     3421 ccagcagaat acctgcgata ggtagtacgg ataccggcag catcagcgat ttaccgacct
     3481 tttgcaggtt agcaaatgca ttcttaaaca taattgagag tgctcctgag tatgggtgct
     3541 ttttttacgt tctcacgcgt ggcaaggggg gagagcctcg ccgtgtacag ggcatctaag
     3601 cgccctttat ttattacaca gagtaaaata attcagtgcc aatttgtttg acggctatca
     3661 cgtttcaact tcagatcatt ggagaagttg cacattttga caaaacatat gtcacaatat
     3721 ttcaaacgcg gttattcacc gcccaatttt cggcggtgaa ctttactcgt tttagccatt
     3781 aattacgagc tttaaaaaaa ctcattcaac ggatggattg aaggcgggaa gcgtcgatgt
     3841 gaaacagacg ggcgaagtta tcggtggtta cctgcgccag ttcttcaacg gcaacacctt
     3901 tcaacacagc catgtattct gcaacgtcac gaaccatcgc aggttgattc tcttttcctc
     3961 gatgcggtac cggcgcaagg taaggtgagt cagtttccac cagtaatcga tccaggggga
     4021 cataacgcgc agcatcgcgc agttgctccg cattacggaa ggtcacaatg ccggaaaagg
     4081 agatgtaaaa tccgagatcc agtaatttac ccgccgtttc tctgtcctct gtaaaacagt
     4141 gtagtacgcc accgcaatcc gtcacttttt cttcgcgcag aattgccagc gtatcggcac
     4201 gggcgtcacg ggtatggacg ataaccggct tgttcagttc acgaccaatc tggatatgat
     4261 ggatgaagga ctcttgctga cgtactttag tttccggtgt gtaataataa tccagcccgg
     4321 tttcacccag cgctacaaca ccctcttctg ccgccagacg gcgtaaatct tctacatcgt
     4381 agggatcatt ctggttgagt ggatgcacgc cacaagaaaa tacgacgttg tcacgttcgc
     4441 ctaccagatc ccgcatatgt aaataacccg gtaatgttgt ggcgactgcc agacaaaatt
     4501 tcacatcgcg tgcggcggct ttcgccagaa cgtcatccac gtccttatgc aaagattcat
     4561 aatccagacc atcgagatgg cagtgtgagt cgactaaaaa catgatgtct ctcttaaaga
     4621 tgaggaaccg gtagcacaac gcccggttgc aggtaatgct caatacgcag taaaagatcg
     4681 gtgatgagaa gctcgcggtt gatgcctgtg acagacatta actgttcacg aatgtggcaa
     4741 acatccccca gtatagcctg caggcgcgag ggagaaagat ggtttgccag ttcggcgacc
     4801 aggcccggca catcaacatt ggtcacctgt gcagcgccat gatggcgttt tagcgcatcc
     4861 atcagcaacg ttgccagcca gtgtaaacgc gccggagctt gttcatgatt aagggccgcc
     4921 agcagcgaat accagtcgcc cgatggtacg ctatatgcca acgcctgaca caatgtttca
     4981 cgagcctgcc agttatctcc ctgaaacaac gccagtgccg cgctaggcga accggcactt
     5041 aagcgcaatg cggcaagtaa tgcatcctgt gacattgtca cttcgcgtga aagccaggtc
     5101 acggtgtact gttccggcgg cggcgcaagg taatgtaacc gacaacgact acgtaatgtt
     5161 gccagtaaac gttcaggctc gcgggtagcc aggaaaaacc aggtttctgc tggtggctct
     5221 tcaagcgttt tcagcaatgc gttagccgcg gcgtcggtca gtaaggcagc atcggttacc
     5281 cagacaactt tcgcaccacc taggcgtgcg tgctcattca gcttttcggt gacctcacgt
     5341 accgcatcaa tgcccagcgt atttttccct ttttcgggag ccagggtgta gtaatcggga
     5401 tgcgtgccag cctgcatcaa ctgacatcca cgacagtgac cgcaactttt gtggccctgc
     5461 ggttgttggc agagtaaata acggctcagg gcgtagatta aagcatcatc gcccatgccc
     5521 ggtaacgcct gaatgagtag cgcatggtga cctcttccgg cctgatagct ggctaccagt
     5581 ttttcgaaat caggtcgtaa ccatggatac catctcatgc gtccaactcc ttcacccagt
     5641 gggtcacggt agtacggatt gcatccatca cggcctccag cggctgggtg gcatcaatgg
     5701 tatgaatact tttatcttgt gcagccagtt ccagatagcg ggcgcgggtg cgattaaaga
     5761 aatcgaaaga ctcttgctca atacgatcca gctcgccgcg cgcacgcgcg cgttttaagc
     5821 caacttccgg ggtaacatcg agatagagcg ttaagtcagg gcgaaaatcc ccgagaacag
     5881 catcacgcag tgttgccagc atatgttggt caataccacg tcctccgccc tgatacgcct
     5941 gagtggagag atcgtggcga tcgccaatca cccaggtgcc gttagccaga gctggtttga
     6001 tgaccgtttc taccagttga acgcgcgcgg cataaaacat cagaacttcg gctttatcgg
     6061 taatgacttc atcgcctacc gatttgatat ccagcaccag gcttcttaac ttttcggcaa
     6121 gttgcgtacc gccaggttcc cgagtgaaaa ccatgtcgcg gatacccagt tgctcaagcg
     6181 tctcaaccac cacattacgc gcggtagttt tgcctgcgcc ttccagcccc tcaatgacga
     6241 tatacttact gcgcattttt ttccttaagc actttcagat aatcctgcac agacttgtta
     6301 tggctggcaa gattggtatt aaacgtgtga ccacctttac catcggccac aaaatagaga
     6361 tacggcgttt ttgccggatg cgcagcagcc ttcagcgaat ccgcccccgg cgtagctatc
     6421 gcacctggcg gcaaaccggt aatggtatag gtgttatacg ctgtcggcgt ttccaggtct
     6481 gcacgagaaa gtttgccatt ataacgctct cccatcccgt aaatcacggt tgggtcggtc
     6541 tgcaggcgca taccaatgcg taaacggttg ataaataccg aggcaacctg atcgcgttca
     6601 ctggcaacgg cggtttcttt ttcgataatt gatgccatcg tcaccagctg gtttttatct
     6661 ttataaggca gaccgtccgc acgcccttcc caggcgctat cgaccgcttt caccattttc
     6721 ttgtgcgctc gcttgagtaa cgcgacatcg gtggtattgg cggtatacat ccaggtgtct
     6781 ggccagaacc aaccttcaat ccactccggg ttttccagtt caagtgcctg cgctacggtg
     6841 gcgtacttat cgtcactcag cgtgtgcttg atatacggag cctcacgcaa ttgcttgagg
     6901 tagtcgctca gacgcatccc ttctaccagt cgcagcggga actgtgcttc tttaccgctt
     6961 tccagcaatt tcagcatctc gcgcacggtc atctgcggtg taaagcggta agtcccggct
     7021 ttaaagtgag aaagatccgg ttcgatacgc agcaaccatt gaaaaacccg tgggcgattg
     7081 atgatcttat cggcataaag ctgttcacca agcgccagac gtccggtccc tggcttcagg
     7141 gtaaatatcg tctcttcttt gataagcaat ttgctgtcgg caagatggcg aaccttccag
     7201 acgcccacac cagcggcgat acccagtacc accagcaata acaagataat caataacact
     7261 tttttcatga ctaattcggg cgctcacaaa gtggggctaa atattcatat aacgttgctg
     7321 acgaaaagga gacatcgcca caggcacgta cgggcatcac tggcattaac gcattacaaa
     7381 taaccatctc atctgcctgc aacgcctctt ccagagaggc ttgcacttcg acaagctgat
     7441 aagaggattg tgccagcaaa cggatacaga attgtcgcat aatgccgtta acacctgcct
     7501 gatccagtcg cggcgtataa actacattgc ccttccgcca gaacaaatta gccgcacagc
     7561 attccgtaac ccacccttcg ctgtcaagga ccagcgcctc atcagcgttt gtctgctcaa
     7621 gatgagagcg gatcaatact tgctcaagcc gattaagatg tttaatacct gcaagatggg
     7681 gattgcgccc cagccgcacc gggcttagcg ccaacgtcat cccttcgtta cgcaaacggt
     7741 cgtaatgtgc aggataaacc gtaacggaga gaatccgcgt tgccggtcca ctgttcaatg
     7801 tgctgtaccc tcgcccgcca ctaccgcgac tgatcacgac tttcagcaca ccattttgct
     7861 gttctgctgc cagcgttttc atctcttgtt caagctgagg ccagaagtca cagaaaatca
     7921 tcaaccgctg acaagcatcc tgtagtcgct ggatatgcgc cgataacaaa ctgactttac
     7981 cgtcgataac tctggcggtg gtaaaacaac catcaccaaa ctgcgttgcc cgatcgctta
     8041 ctgccagcga ttcctgctta tgaccgttaa ttaagaacat accggctcct tatcagagaa
     8101 ttgttagtgt ggcagcatgc tcactacgga acaagtcgga ataaaagcta agaaaaaagg
     8161 cccgcaagcg gaccttttat atgggtggga aatgacaact tagatctttt taaagatcaa
     8221 agaaccatta gtgccaccga agccgaagga gttacacagc gtgtattcca ttccgctgac
     8281 ctgacgtgct tcatgcggta cgaaatccag atcgcaacct tcatccaggt tatccaggtt
     8341 gatggttggc ggaacagcct gatcgcgcag cgccaggatg gagtagatag actctactgc
     8401 ccccgccgca cctaacaggt gaccggtcat agatttcgtg gagcttacca acacacggct
     8461 tgcagcttca ccgaagatgg ctttcaccgc ctgcgcttca gctttatcgc cagccggcgt
     8521 agaagtaccg tgcgcgttaa cgtagccaat ctgactcgct tcaatgcctg catcacgcag
     8581 agcatttgcc attgccagag ctgcgcctgc gccattttct ggcggtgacg tcatatgata
     8641 agcatcgctg ctcataccaa agccgacgag ttcagcgtaa attttcgcac cgcgtttttt
     8701 cgcgtgttcg tactcttcta gtaccagcat accggcacca tcgcccagta cgaagccatc
     8761 acgctcttta tcccacgggc ggctcgccgc ttgcgggtta tcattgcggg tagataatgc
     8821 acgtgccgcg ccaaaaccac caacgcccag cggcgtactg gctttctctg cgccacctgc
     8881 aaccatcacg tcagcatcgc catacgcgat aatacgcgca gcatgaccaa tgttgtgcac
     8941 gccggaagta caggcggtcg cgatagagat gcttgggcca cgcaggccat acatgatagt
     9001 cagatgacct gccaccatgt tcacaatcgt tgacggaacg aagaatgggc tgatcttacg
     9061 tggaccaccg ttcatcagag atgtgtggtt ttcttcgatc agtccgaggc cgccaatccc
     9121 ggagccaatt gcggcaccaa tgcgggttgc gttctcttcc gttatttcaa ggccagaatc
     9181 ctgcatggcc tgaacgccag cgacaattcc atattgaatg aaggcatcca tcttgcgctg
     9241 ttctttgcgc gagataatgt cctcacagtt aaaatccttt actaagccag caaatttcgt
     9301 tgcataggcg ctagtatcga aatggtcgat taggctgatg ccactctgac cggcaagcag
     9361 agctttccag gtagactcta cggtattgcc gacaggagac aacatgccca gtccggtcac
     9421 aactacacga cgcttagaca cgtttgtcct ccagggaggg aaaaaatgat tctagtggga
     9481 caaaaagata aaactcaggc ggtcgaacga ccgcctggag atgttcactt acgcctggtg
     9541 gccgttgatg taatcaatgg cagcctgaac ggtggtgatt ttctcagctt cttcgtccgg
     9601 aatctcagta tcaaactctt cttccagagc cattaccagc tcaacggtgt caagagaatc
     9661 cgcgcccagg tcttcaacga aagaagcatt gttggtaact tcttcctgct taacgcccag
     9721 ctgttcgccg ataattttct taacgcgttc ttcgatagtg ctcatactct taaatttcct
     9781 atcaaaactc gctttcgcga tggttttcgt agtgtataaa atgttgaaaa atttgcaact
     9841 aaatcccggc aggtcttacc acgattttac gttattttga ggccttaccc cccaataacg
     9901 caaatcattt tgcactaatt attactcata accacgcaga ctgcgcgcaa catgagcatt
     9961 ttgtgcaaat cgcggtcaga ccatgtacat cccgccgttc acatgcaaag tttcacccgt
    10021 gatgtaagct gcttcgtcgg atgccaggaa tgcaaccgcg ttggcgattt cctgtgcgcc
    10081 gccgaggcga cccgcaggaa cctgcgccag gatacccgca cgctggtcat cgctcagcgc
    10141 acgtgtcatg tccgtttcaa taaagcccgg agcaacaacg tttacagtaa taccgcgtga
    10201 cgcaacttcg cgcgccagtg atttactgaa gccgatcaag cccgctttcg ccgcagcgta
    10261 gttggcctga ccgccatttc ccatggtacc aaccacagaa ccgatagtga taatacgacc
    10321 atgacgcttt ttcatcatag cgcgcattac cgcttttgac agacggaaaa cagatgaaag
    10381 gttggtttcg ataatatcgt tccactcttc atctttcatt cgcattaaca ggttatcacg
    10441 agtgataccg gcattattga ccaggatatc cacttcacca aattctgcgc gaattttttc
    10501 cagaacagat tcgatagatg ccgggtcggt cacattcaac atcagacctt tgccgttagc
    10561 acctaaataa tcactgatcg cctgagcgcc attttcactg gtcgcagtgc caataacttt
    10621 cgcgccacgg gctgcgagcg tttcagcaat tgcgcggcca attccgcggc ttgcaccggt
    10681 taccagtgcg atttttcctt caaaattcat gattttcctc ttttaaagct cgagcgccgc
    10741 tgccatcgct gaaggttcgt tcagcgccga ggcggtcagg gtgtcgacaa tgcgtttcgt
    10801 caggccagtt agcactttgc ccgggccgac ttcatagaga tgttctacgc cttgcgctgc
    10861 catgtactca acagacttcg tccactgaac cgggttatac aactgacgta ccagtgcgtc
    10921 acgaatggca tcaccattgg tttcgcattt cacatcaacg ttattcacaa caggaactgt
    10981 tggtgcgtta aaggtgattt tcgctaattc tactgccagt ttgtcggctg ctggtttcat
    11041 cagcgcacag tgagacggta cgctcactgg taacggcagc gcgcgttttg cgcccgcagc
    11101 tttacaggca gcgccagcac gctcaaccgc ctctttatga ccggcaataa ccacctgtcc
    11161 cggagagtta aagtttaccg gagaaacgac ctgaccttct gcagcttctt cacacgcttt
    11221 cgcaatagac gcatcatcca gaccgatgat tgccgccata gcgcccgtgc cttctggtac
    11281 ggcttcttgc atgaacttgc cgcgcatctc aaccagacgc accgcatcag cgaaatcaat
    11341 cacgccagcg caaaccaacg cggagtattc ccccaggctg tgaccggcca tcattgccgg
    11401 tgctttaccg ccctgctgct gccatacgcg atacagcgca acagatgcag tcaacagcgc
    11461 tggctgggtt tgccaggttt tattcagttc ttcagctggc ccctgctggg tcagcgccca
    11521 caggtcgtag cccagcgccg cagaagcttc agcaaacgtt tcttcgacaa ttggatagct
    11581 cgccgccata tcagccagca ttccaacggt ttgagaaccc tgtccaggga acacaaatgc
    11641 aaattgcgtc atgttttaat ccttatccta gaaacgaacc agcgcggagc cccaggtgaa
    11701 tccaccgcca aaggcttcaa gcagaaccaa ctgccccggc ttaatgcgcc cgtcgcgtac
    11761 agcttcatcc agcgcgcacg ggacagaggc cgcagaggta ttaccgtggc gatccaacgt
    11821 caccacgaca ttgtccatag acataccgag ttttttcgcc gttgcactga taatacgcag
    11881 gttagcctga tgcggaacca gccagtccag ttgagaacgg tcaagattat tcgccgccag
    11941 cgtctcatca acgatgtgcg ccagttccgt taccgcaacc ttgaagactt cgttgcccgc
    12001 catcgtcaga tgaattgaat tctctggatt cacgcggtcg gcgtttggca gcgtcagcaa
    12061 ttcaccataa ctaccgtcgg catgcagatg ggtggagatg attcccggct cttcagaggc
    12121 agccagcacc gcagcgcccg cgccatcgcc aaaaataata atagtcccac gatctgttgg
    12181 atcgcaggtg cgcgccagta catcggaacc gacgaccaga gcatacttca ccgccccaga
    12241 tttcacgtat tgattggcta cgcttaatgc ataggtgaaa cctgcgcagg ctgctgcaac
    12301 gtcaaatgcc gggcaacctt taatgcccaa catgctttga atctgacaag ctgcgctcgg
    12361 gaaagcgtgc gtagcagaag tcgttgccac aacgatcagg ccaatctggt ctttctcaat
    12421 gcccgccatc tcaattgcgc gtgtcgccgc ttcaaagccc atggttgaaa cggtttcgtt
    12481 tggcgcggca atgtggcgtt cgcggatacc ggtacgagtg acaatccact cgtcagaggt
    12541 gtccaccatt ttttccaaat cggcgtttgt ccgcacttgt tcgggcagat agctgccagt
    12601 accaataatc ttcgtataca tgtacgctca gtcacttttc ggttatatac cgtcacttgc
    12661 aaactgcgag ttcgctggca gcgtcctgct accgcagagt tccgcttttg ccaccgtcca
    12721 gcagctcaaa accagctggg tatacagatt ccaggcgagc ggcaattcgc tgaggaactt
    12781 gtcgctgcac cgcctgcact gcctgttcaa tcgcgaccgc aaaagctcgc tgattggctg
    12841 caccatgact ttttatcacc gtgccgcgca atcctaacag acaggcgccg ttatactggt
    12901 cggggttgag gtgactgaat cgcctcgtca ggctcttttg tagccaacgc tttaataaca
    12961 gtagccacca cgaccgtttt ttcccttcac cctgagattt cagcagagaa aggaacatcc
    13021 tgacaacacc ttccatcgtc tttaacgtga catttcctgt aaagccgtca caaaccagca
    13081 catctgtctt gccagttaac aactcattgg cttcaagata gccgatataa ttgatagaag
    13141 ggattgtttt aagcaccgct gaggcatccc gaatactgtc gagacccttt acttcttctt
    13201 caccaatatt gagcaacgcc acgcgaggat tgggaatttc caccacctct tcagccagaa
    13261 ctgagcccat aatggcaaat tgcaccaaca ttgtgctgtc acaatcgacg ttggccccta
    13321 aatcgaggac caccgttttg cccttttgct gatgtggtaa taccgtcacc agcgccggac
    13381 gctcaatccc ctccaggggc ctgagtaata attttgccag ccccatcagt gccccggtat
    13441 taccggcact gacacaggct tgcgctcgac cttctttcac cagctccagg gccatacgca
    13501 ttgaactccc acgactagcg cggatagctt gcgaaggccg ggcatcactg gcgataactg
    13561 actgcgcagg aataatctgc agacgcgaac gttgttcaaa gtcagcttta gcaagtaatg
    13621 gcgtgatggc gtcgggattg ccgactaaaa gaagagtgag ttgcgaatta gaattcagtg
    13681 cctgcaatgc tgcaggcact gtcacggaag ggccaaaatc ccctcccatg acatctaacg
    13741 ccagggttag acgtgtcaag gtatcgtcgc cgcccggttt ggtctttccc cagttgcctg
    13801 gggaaaatcc tcactaagct tcatcacgca gacgcgtgat tacttagcga tgaccttgcg
    13861 gccgcggtag taaccgtcgg cagtgatatg gtgacgcagg tgtttttcac cagaagtttt
    13921 gtctacagac aggctggtga ctgcggtcag cgcgtcatgg gaacgacgca tgccacgttt
    13981 ggaacgggtt ggtttattct gttgtacggc catggacctt actcctcaat tacggtttat
    14041 ccccgatcca acggggagca ccaattactt acgctttaag ctggctaata cggcaaatgg
    14101 gtttggcttt tgcacttctt caggcagttc accaaagacc atgtccgctt cggacacttc
    14161 acagtgttca gaatcatgca ccggaactac cggcaaggcg aggatgattt catcttcaac
    14221 cattgcaagc agatcgattt caccgaattc gttaacctca atcggttcat acgcttccgg
    14281 cagtgcttca gcctgttcgt ctgaacgcac aggactaaaa caatacgttg tgtagacctg
    14341 atgagtaaac ggcttcccgc aacgctgaca ctcgagcgtt accgtcacct tcgcatcgcc
    14401 gtttaacact gcgagacgtt ggttatcgat agcgaacgac atggagcatt ccacatcact
    14461 gtccacactg actacggatt cggcgacgcg ctcaacctga tcaggggtat agataccctg
    14521 gtaatcaagg cgtttttgag ccgtacgaac cggatcgaga gtcaggggta attttacctt
    14581 ttgcataggg cgcgcatatt aactttgtaa cgtcatagag tcaaagaaaa aggcagcctg
    14641 tggttgcctt ttgccaataa ttcgcacaca ttgcgggtta ctgctttatt ttcgttcagt
    14701 gctgataacg aacatcaacc tgcaaaacga agcgcatagt ttaaaatggc tatcatcaat
    14761 acgctatatc tgatggaaaa aaatatgcct aaacttattt tagcctccac ctcgccatgg
    14821 cgtcgcgctc ttctggaaaa actgcaaatc tcttttgaat gtgcagcacc agaggtcgac
    14881 gaaaccccac gcagcgacga atcaccgcga cagttggtgc ttcgactggc acaagaaaaa
    14941 gcgcaatctc tggcgtcacg ttatccggat catttaatta ttggttcaga ccaggtatgt
    15001 gttcttgatg gcgaaatcac cggtaaaccg ttaacggaag aaaatgcccg tctgcaatta
    15061 cgcaaagcca gcggcaatat cgtcaccttc tatactggac tggcgttgtt taattcggcg
    15121 aatgggcatc tacaaacaga agtggagcct tttgacgtcc atttccgtca cctgtgcgag
    15181 gcggagattg ataattacgt gcgtaaggag catcccctgc actgcgcggg tagctttaag
    15241 agtgaaggat ttggcattac gctgtttgag cgtttagagg gacgtgaccc taacacgctg
    15301 gttggtttac cgctaatcgc gctgtgtcag atgttacgtc gggaagggaa aaacccgctg
    15361 atgggatagt tgtttgcgcc ggatgcggtt acgtcgcatc cggcaatcgg tgcaggcggc
    15421 ctgatgcggc gtaaacgcct tatcaggcca accgcccatt atcaggctta tatgcttgta
    15481 ttagcgcgcg ttacgcagct tttgcaaaca acgcttcaaa ccttcatcca tcggcgcttc
    15541 gatacgcatc acctcaccgg tccccggatg ggtaaacttc aacgctgcgg cgtgcaggaa
    15601 cagacgattt aatcccgtgc ctgcttcagt gagctgtctg tcaaattcac ggtcaccgta
    15661 gcgatcgtca aaggcaatcg gatgacccgc atactgtgta tgcacacgga tctgatgagt
    15721 acgtccggtt accgggctac aacgcaccag ggtggcaaat gcatagcgtt cttccacttt
    15781 aaagcgtgtt tccgacggtt tgccttcctg actgacacgc acgatacgtt cgccgctttg
    15841 cagaatattt ttcaataacg gcgcttgaac gctcttcaca tgcgactgcc actgactgcg
    15901 caccagcgcc aggtaatctt tttgcattcc tttttcacgt aattgctcat gcagagaacg
    15961 caacgccgag cgttttttcg ctaccagcaa cacacctgaa gtgtcccggt cgagacgatg
    16021 aaccagttca aggaaccgcg cttccgggcg caacgcccgc aaaccttcga taacgccgaa
    16081 gcttaaacca ctgccgccat gtaccgccgt accggaaggt ttattcagca ccaggatatg
    16141 atcatcttca tataagatga cgtccgccag cgccgccacc ttttgcagat gtggcgaaac
    16201 cgcctcttct tcccgctcag caacgcgaac cggtgggata cgcacctcat cacccgcttc
    16261 gagtttatat tcaggcttaa tacgtttttt gttcacccgc acttcgcctt tacgcaaaat
    16321 acggtaaatc atactttttg gtacgccttt caattgggta cgcaaaaagt tgtcgatacg
    16381 ttgccccgct tcgtcagcgg tgatagcaac aatttttacg gatggagtct ctgttttcat
    16441 ggtgggcgat tctaattagc caacaggatt cgcgccactc atttttctat gcttatattt
    16501 agttttacac cttattactt cactacgtga tcactttatt gatggttatt aaaccaatca
    16561 ccagcaagaa gtgaaaaatg taatgaacgc atcccacaca attaaacacg gagcagtatg
    16621 atggttaaaa gggtgaaact ggcaggtgtg gagcctcgca gatttccggc atcatagtgc
    16681 ccaatacggg atgcataaag ctccctcaga ctggagactc cacaaatgaa atatacaccg
    16741 gttggcgttg gtatcgcaaa acatgtcatt cagattcact tcatcaatga gcacacag
//
LOCUS       DAZPEB010000078        16674 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZPEB010000078 DAZPEB010000000
VERSION     DAZPEB010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16674
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.079"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            246..665
                     /locus_tag="ACHURJ_002217"
     CDS             246..665
                     /locus_tag="ACHURJ_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFR1281159.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            665..1932
                     /gene="umuC"
                     /locus_tag="ACHURJ_002218"
                     /pseudo
     CDS             665..1932
                     /gene="umuC"
                     /locus_tag="ACHURJ_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
     gene            complement(1978..2508)
                     /gene="dsbB"
                     /locus_tag="ACHURJ_002219"
     CDS             complement(1978..2508)
                     /gene="dsbB"
                     /locus_tag="ACHURJ_002219"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFR1281160.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     MISQPFKAKKRDLFGR"
     gene            complement(2654..4195)
                     /gene="nhaB"
                     /locus_tag="ACHURJ_002220"
     CDS             complement(2654..4195)
                     /gene="nhaB"
                     /locus_tag="ACHURJ_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFR1281161.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            4417..5136
                     /gene="fadR"
                     /locus_tag="ACHURJ_002221"
     CDS             4417..5136
                     /gene="fadR"
                     /locus_tag="ACHURJ_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFR1281162.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(5188..6720)
                     /locus_tag="ACHURJ_002222"
     CDS             complement(5188..6720)
                     /locus_tag="ACHURJ_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFR1281163.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSVELLERCPPRMG
                     NL"
     gene            7050..8348
                     /gene="dadA"
                     /locus_tag="ACHURJ_002223"
     CDS             7050..8348
                     /gene="dadA"
                     /locus_tag="ACHURJ_002223"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFR1281164.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFDSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            8358..9417
                     /gene="dadX"
                     /locus_tag="ACHURJ_002224"
                     /pseudo
     CDS             8358..9417
                     /gene="dadX"
                     /locus_tag="ACHURJ_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
     gene            complement(9804..11540)
                     /locus_tag="ACHURJ_002225"
     CDS             complement(9804..11540)
                     /locus_tag="ACHURJ_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFR1281165.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGTAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(11635..12549)
                     /gene="ldcA"
                     /locus_tag="ACHURJ_002226"
     CDS             complement(11635..12549)
                     /gene="ldcA"
                     /locus_tag="ACHURJ_002226"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFR1281166.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNKTFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVSAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            12649..13260
                     /gene="emtA"
                     /locus_tag="ACHURJ_002227"
     CDS             12649..13260
                     /gene="emtA"
                     /locus_tag="ACHURJ_002227"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFR1281167.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(13262..13996)
                     /locus_tag="ACHURJ_002228"
     CDS             complement(13262..13996)
                     /locus_tag="ACHURJ_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFR1281168.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            14197..14451
                     /locus_tag="ACHURJ_002229"
     CDS             14197..14451
                     /locus_tag="ACHURJ_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFR1281169.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFQTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGLFRLLRRE"
     gene            14630..15070
                     /gene="ycgY"
                     /locus_tag="ACHURJ_002230"
     CDS             14630..15070
                     /gene="ycgY"
                     /locus_tag="ACHURJ_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcgY"
                     /protein_id="HFR1281170.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALY
                     RQRRCKSPGDDSETLTRRNDAIFGNHVWQTFAQYFPPGLEKPAV"
     gene            complement(15149..16674)
                     /locus_tag="ACHURJ_002231"
                     /pseudo
     CDS             complement(15149..16674)
                     /locus_tag="ACHURJ_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
BASE COUNT         4185 a         4138 c         4353 g         3998 t
ORIGIN      
        1 tgataaatat caaacaatgt ttaatgtcat tatggcgaat gcttctattc tatttttttg
       61 ccgggtgata tttttcattt ctgctggatg agcgtcgtcg ccagaaggcc acgtgagcac
      121 aggataagag aacgaaaaat cagcagtcta tacagcgaca aatattgata gcctgaatca
      181 gtattgatct gctggcaaga atagactact gtatataaaa acagtataac ttcaggcaga
      241 ttattatgtt gtttatcaag cctgcggatc tccgcgaaat tgtgactttt ccgctattta
      301 gcgatcttgt tcagtgtggc tttccttcac cggcagcaga ttacgtcgaa cagcgcatcg
      361 atctgaatca actgttgatc cagcatccca gcgcgactta cttcgtcaaa gcaagtggtg
      421 attctatgat tgatggtgga attagtgacg gtgatttact gattgtcgat agcgctatta
      481 ccgccagcca tggtgatatt gtcatcgctg ctgttgacgg cgagtttacg gtgaaaaaat
      541 tgcaactacg cccgacggta cagcttattc ccatgaacag tgcgtactcg cccattacca
      601 tcagtagcga agatacgctg gatgtctttg gtgtggtgat ccacgtcgtt aaggcgatgc
      661 gctgatgttt gctctctgtg atgtaaacgc gttttatgcc agctgtgaga cggtgtttcg
      721 ccctgattta tggggtaaac cggtggttgt actatcgaat aatgacggtt gcgttatcgc
      781 ccgaaacgct gaggcaaagg cgctgggtgt taaaatgggc gatccctggt tcaaacaaaa
      841 agatctgttt cgtcgctgtg gtgtggtttg ctttagcagc aattatgagc tttacgcaga
      901 catgagcaat cgggttatgt cgacgctgga agaactatcg ccccgcgtcg agatttacag
      961 tattgatgag gcattctgcg atctgacagg tgtgcgtaat tgtcgcgatc tgactgattt
     1021 tggcagagaa attcgcgcaa cggtgctaca acgtacccat cttactgttg gtgtggggat
     1081 cgcccagacc aaaacgctgg ctaagcttgc caatcatgcg gcaaaaaaat ggcagcggca
     1141 gacggggggg tggtggattt atcaaatctg gaacgccagc gtaaattaat gtctgctctc
     1201 cccgtggatg acgtctgggg gattggacgg cggatcagca aaaaactgga cgcgatgggg
     1261 atcaaaaccg ttcttgattt ggcggataca gatatccggt ttatccgtaa acattttaat
     1321 gtcgtgctcg aaagaacggt gcgtgaactg cgcggtgaac cctgtttgca actggaagaa
     1381 tttgcaccga caaagcagga aattatctgt tcccgctcgt ttggtgaacg tatcacggat
     1441 tatacgtcga tgcggcaggc catttgtagt tacgctgccc gggcggcgga aaaacttcgc
     1501 agtgagcatc aatattgtcg gtttatctcc acatttatta agacgtcacc atttgcgctc
     1561 aatgaacctt attacggcaa tagcgcgtcg gtaaaactgc tgacgcccac tcaggacagc
     1621 agggatatca ttaacgccgc tacgcgatct ctggatgcca tctggcaagc gggccatcgc
     1681 taccaaaaag cgggcgtgat gctgggggat ttcttcagtc agggagtcgc gcagctcaat
     1741 ttattcgatg acaacgcacc gcgccccggg agtgagcaat tgatgacggt aatggataca
     1801 ctgaatgcta aagagggcag aggaacactc tattttgccg ggcaggggat ccaacaacaa
     1861 tggcagatga agcgagccat gctttcacca cgttatacaa cgcgaagttc tgatttactg
     1921 cgggtcaaat aaatatagcg gcaggaaaaa agtgctcccg caggagcgcc gaatggatta
     1981 gcgaccgaac agatcacgtt ttttcgcttt aaacggctgg gaaatcatca ccagcaccgc
     2041 gacaatcagg taagcgataa aaataccgag cagccactgc ggcatttcca gacctaaaaa
     2101 atcccactga cgctcggcgc aatcgccaga ggcgacaaac acttgcggca cccatttatc
     2161 cagcggcagc cattccggga aacgaaccat aaaatcacag gtggcaaacg gcgaaggata
     2221 gagctgaagc atggtgtgct cgtaagttaa ctgcacaccg cggaacgcac tatacaacca
     2281 gataaccatc gctacataac gcagcggagt tttcggggcg atcgcgccaa tcagcgcagc
     2341 acccagaacg ccgaataacg cgcagcgttc ataaatacag agcacgcaag gtttcagtaa
     2401 catcacatgc tggaaccaca gcgccgtcag ttccagtgcc agagcagtaa acgccatcaa
     2461 cagccacgcg ccccggcctt gtgaacattg gttcaaaaat cgcaacataa tcatttccct
     2521 gcaatatgca tagagtgcgc agtttaaacc aattcattcg ctgcgccacc aggaggcagc
     2581 aaaaatagga cgcaattgga caattatcgg gcgaaaaggc aaaccgggca tttatatgcc
     2641 cggtaagttg ttatcaaagc gttgctatcc agcccatttg cataaaccat tcggttacag
     2701 gggcaagcgt aaactcgacg cagagcagcc cgacgagtgt caggacgagg gtgtaaggca
     2761 gggccatcca caccatgcgg ccataagaga ggcgaatcaa tggcgcgagt gcagaggtca
     2821 gcaggaacag gaacgcagcc tgaccgttcg gcgtagcgac agagggcaga ttggtaccgg
     2881 tattaatggc gaccgccagc agctcgtatt gcttcaacgt gatagcgcca ctttccattg
     2941 ccgctttcgc ttcgttgata taaatcgtcc ccacgaagac gttatccgaa atggatgaca
     3001 gcagaccgtt gaaaatatag aacaacgaca gctgagcatg ttccgatgcc tgcaacacaa
     3061 actgaataat cggcgaaaac agttgttggt cgataatcac cgcgactacc gagaaaaaca
     3121 ccgtcaacag tgcggtgaat ggcagagatt cggtgaaggc tttaccgata gcatgttcat
     3181 cggtgacacc ggtcaatgat gtcgccagaa taatgaccga caaaccaatc aagccaacct
     3241 ccgccagatg caacgccagc gcagtcacca gccagacgcc gataatcgcc tggacaatca
     3301 gacggatttt atcctgacgg gtacgctggt ggcggctttg atcgtcaaac tgttgcaaca
     3361 cttcgcggac tttctccggc agcgtttcac cgtagccaaa ccaacgcagc ttctctacca
     3421 gcaggcaggt taacaggcca caaatcagaa ccggaacggt caccggcgac atgcgcagga
     3481 agaaatcgcc aaaatgccag ccagccgctt tagcgatgat caggttctgt ggttcgccca
     3541 ccatggtcat tacgccgcct aatgcggtgc cgacaccggc atgcatcatc aggctacgca
     3601 gaaagccacg gaactgttcc agaaccactt tgtaatgctt gtcgatatga ctatcgtctt
     3661 gcaggtcggt atcttcagta cgggaagagg ctacgcggtg ataaatacca taaaaaccga
     3721 ctgcaacgct gatcaccacc gccacgacgg ttaaggcatc gaggaacgcg gagaggaacg
     3781 ccgccgccac gcaaaaagag agcgacagca gcattttgga acgaatgctt aacagcaaac
     3841 gggtaaatat gaacagcaat agctgtttca taaaatagat acccgccacc ataaacatca
     3901 gtaacagcaa gacttcaaga tttgccgcca cctcttcacg gacgtgttcc gcgctggtca
     3961 tgccgatgaa taccgcttca atagccaaca gaccaccggg gagtagcggg tagcatttca
     4021 gggccatcgc cagagtgaaa ataaattccg ctaccagcaa ccagccagcg acgaaagggc
     4081 tgatgaggaa aattaacgga tttacgatta agaaaatgat gagggcgagt ttgtaccagt
     4141 cgggggactg gcccaaaaaa ttgcgccata gcgcgcggcc ccaggagatc tccatgatgg
     4201 tttcccttac cttacaaata atcaatgatg tttttatgtt taaacgcaaa gcttaacggt
     4261 caggcaggag tgaggcaagt cttgatagtc aaggggaaag agatgcggaa aatgaagcct
     4321 tgatcccttt tttcttcttt ttgtctgcta tcagcgtagt tagccctctg gtatgatgag
     4381 tccaactttg ttttgctgtg ttatggaaat ctcactatgg tcattaaggc gcaaagcccg
     4441 gcgggtttcg cggaagagta cattattgaa agtatctgga ataaccgctt ccctcccggg
     4501 actattttgc ccgcagaacg tgaactttca gaattaattg gcgtaacgcg tactacgtta
     4561 cgtgaagtgt tacagcgtct ggcacgagat ggctggttga ccattcaaca tggcaagccg
     4621 acgaaggtga ataatttctg ggaaacctct ggtttaaata tccttgaaac actggcgcga
     4681 ctggatcacg aaagtgtgcc gcagcttatt gataatttgc tgtcggtgcg taccaatatt
     4741 tccactattt ttattcgcac cgcgtttcgt cagcatcccg ataaagcgca ggaagtgctg
     4801 gctaccgcta atgaagtggc cgatcacgcc gatgcctttg ccgagctgga ttacaacata
     4861 ttccgcggcc tggcgtttgc ttccggcaac ccgatttacg gtctgatcct taacgggatg
     4921 aaagggctgt atacgcgcat tggtcgtcac tatttcgcca atccggaagc gcgcagtctg
     4981 gcgctgggct tctaccacaa actgtcggcg ttgtgcagtg aaggcgcgca cgatcaggtg
     5041 tacgaaacag tgcgtcgcta tgggcatgag agtggcgaga tttggcaccg gatgcagaaa
     5101 aatctgccgg gtgatttagc cattcagggg cgataatccc ttccgtttaa aaagcaaacc
     5161 cctcaaacga ggggtttttt gttgttttta cagatttccc attcttggcg ggcaacgttc
     5221 cagcaactcg acgctgccat cttcgttttg ctgttccagc atcacatcaa atccccacag
     5281 gcgatgcaca tgcttcagca cttctttgcg cccccgatcc agcggtgcgc gattatgtgg
     5341 aatgtaacgc agcgtcagcg aacggtcgcc gcgcaaatcc acgttccaga tctgaatatt
     5401 cggctccaga ttacttaagt tatattgcga cgataaccgg ttacggatct cccgataacc
     5461 ttcttcatta tgaatagcgg aaatctccag ataattatgc cgatcgtcat ccagcacggt
     5521 gaagaagcgg aaatcacgca tcactttcgg tgacagaaac tggctgataa aactctcatc
     5581 tttgaaatca cgcatcgcga aatgcagcgt ttccagccag tcggaaccgg cgatatccgg
     5641 gaaccagtat ttgtcttctt ccgttggcga ctgacaaatc cgtttaatat cctggaacat
     5701 ggcgaacccg agggcatacg ggttgatgcc gctgtaccac gggctgttat aggggggctg
     5761 gaagaccaca ttggtgtggc tgtgcaaaaa ctccagcata aaacgttccg ttactttccc
     5821 ttcatcatac agatggttaa ggatggtgta gtgccagaag gtggcccagc cttcgttcat
     5881 cacctgagtc tgtttttgcg gataaaaata ctggctcacc ttacgcacaa tacgcaggat
     5941 ttcacgctgc catgattcca gcagtggcgc atttttctcc ataaaataga gcaggttttc
     6001 ttgtggttcg gacggatagc ggcgcgcttc agcaaccgtt ttctcttcct cgcgcttcgg
     6061 cagggtacgc cagagcatat tgacctgact ttgtagatac tcttcgcgac ttttctgccg
     6121 ggctttctct tcttgcagcg agattttttg cgggcgtttg taccggtcca cgccgtagtt
     6181 catcagcgcg tggcacgagt ccagaagccg ttctacttca tccacgccat aacgttcttc
     6241 gcactcggta atatatttac gggcaaaaat cagataatcg acaatcgaac tggcgtcggt
     6301 ccaactgcgg aataagtaat tgtttttaaa gaaagagtta tgcccatagc aggcgtgtgc
     6361 catcaccagc gcttgcatgg taatggtgtt ctcttccatc aggtaagcga tacacgggtt
     6421 agagttaatg acgatttcat aggccagtcc ttgctgaccg tgcttataca gccgttcagt
     6481 ctcgataaac tttttaccga atgaccagtg cggatagtta attggcatgc cgacgctgga
     6541 gtaggcatcc atcatctgtt ctgaggttat cacttcaatc tggtgcgggt aggtatccag
     6601 ccggtagagt ttcgccaccc ggtctatctc tgccagataa acatccagca ggtcgaacgt
     6661 ccagtcgggt ccatcgctca accgtgtggt gtccttattc atagaatcga tcgtcgccat
     6721 acgcgcacct cattgttgtc ggcgctctct gtgtggagca cctcatttca agcatagaac
     6781 acctgttaaa aaccgcgtcg ccggagaatt tttttctttg cgatttctta ttatcagagt
     6841 gccactaatc cgcttctgaa cggaatttta tgctggataa aaagggcgtt cagcaggaga
     6901 tactaaagac gccatattgc cgcagagtca gggagatgtg agccagctca ccataaaaaa
     6961 gctgtatgtt gaataatatt ttcaactgag ttatcaagat gtaattagat tattattctt
     7021 ttactgtatc taccgttatc ggagtggcta tgcgagttgt catactggga agtggtgtgg
     7081 taggcgttgc cagcgcctgg tacttaaatc aggcaggaca tgaggtcacc gtcattgatc
     7141 gggagccggg ggccgccctg gaaaccagcg ccgcgaatgc cgggcaaatc tcccccggat
     7201 atgctgcacc gtgggcggca ccaggtgtgc ctttaaaagc gatcaaatgg atgttccagc
     7261 gccatgcgcc gctggcggtt cgtctcgacg gtacgcagtt tcagttgaaa tggatgtggc
     7321 aaatgttacg taactgcgac accagccact acatggaaaa caaagggcgg atggtgcgtc
     7381 tggcggaata cagccgtgat tgcctgaaag cattgcgcgc cgaaaccaat attcagtatg
     7441 aagggcgtca gggtgggacg ctgcaactgt tccgtaccga acaacagtat gaaaatgcga
     7501 cccgcgatat cgccgtgctg gaagatgccg gcgtaccgta tcagctgctg gaatccagcc
     7561 gcctggcgga agtggagcca gcgctggcag aagtagcgca caaactgacg ggtggtctgc
     7621 aactgcccaa tgatgaaacc ggagactgcc agctgtttac ccagaatctg gcgcggatgg
     7681 cagagcaggc gggggttaaa ttccgcttta atacgcccgt tgaccaactg ctttgcgacg
     7741 gcgagcaaat ctacggcgtg aagtgtggcg atgaagtgat taaggccgat gcgtatgtga
     7801 tggcgtttga ttcttactcg acggcgatgc tcaaaggcat tgttgatatt ccggtgtatc
     7861 cgctgaaagg ctactcgctg actattccaa ttgcgcagga agatggtgcg ccagtatcca
     7921 ccattcttga tgaaacctac aaaatcgcca ttacccgttt cgataaccgc attcgtgttg
     7981 gcggaatggc ggaaattgtt ggttttaaca ccgagctgtt gcaaccgcgt cgtgaaacgc
     8041 tggaaatggt ggttcgcgat ctctatccac gcggcggtca tgtcgagcag gcgactttct
     8101 ggacgggttt gcgcccgatg acgccagacg gtacgccggt tgtcgggcgt acacgcttta
     8161 aaaatctgtg gctgaatacc ggtcacggca cgctcggctg gacgatggct tgcggttccg
     8221 gtcagttgtt aagcgatctg ctctctggtc gcacgccagc gatcccatat gaggatctaa
     8281 gcgtagcgcg ctacagccgt ggatttacgc catcacgtcc gggccattta catggcgcac
     8341 acagctaagg aaacgagatg acccgtccga tacaggccag cctcgatctg caggcattaa
     8401 aacagaatct gtccattgtc cgccaggccg cgccgcacgc gggtggtaaa agcgaacgct
     8461 tacgggcatg gtattgagcg tatctggagc gcgctcgggg ccaccgatgg ctttgcatta
     8521 cttaacctgg aagaggcaat aacgttacgt gagcgcggct ggaaggggcc gatcctgatg
     8581 ctggaaggat ttttccatgc tcaggatctg gagatttatg accagcaccg cctgaccacc
     8641 tgcgtacaca gcaactggca gctcaaagca ctgcaaaatg cgcggctaaa agcaccgttg
     8701 gatatttatc ttaaagtgaa cagtgggatg aatcggttgg gcttccagcc cgatcgcgtg
     8761 cttaccgtct ggcagcagtt gcgggcaatg gcgaatgttg gcgagatgac cctgatgtcg
     8821 cattttgccg aagcggaaca tcctgatgga atttccagcg cgatggcgcg tattgagcag
     8881 gcggcggaag ggctggagtg tcggcgttcg ttgtccaatt cggcggcgac tctgtggcac
     8941 ccggaagcgc attttgactg ggttcggcct ggcattattt tgtatggcgc ttcgccgtcc
     9001 ggtcagtggc gtgatatcgc caataccgga ttacgtccgg tgatgacgct aagcagtgag
     9061 attattggtg tccagacgct aaaagcgggt gagcgtgtgg gctacggcgg tcgctatact
     9121 gcgcgcgatg aacagcgaat cggcattgtc gccgcagggt acgccgacgg ttatccgcgc
     9181 cacgcgccta ccggtacccc tgttttagtg gacggcgtgc gcaccatgac ggtggggacc
     9241 gtctcgatgg atatgctggc ggtcgattta acgccttgcc cgcaggcggg tattggtacg
     9301 ccggttgagc tgtggggcaa ggagatcaaa attgatgatg tcgccgccgc tgccggaacg
     9361 gtgggctatg agttgatgtg cgcgctggcg ctacgcgtcc cggttgtgac ggtgtaactt
     9421 gttgtaagcc ggatcggagg caacgtcttc tgggtgcaaa aaaaatcatc catccggctg
     9481 gtcagcaact gtagttgtta atgtgacaga gccattgccc atgatagtgt ccattaaaag
     9541 gatggacact atttccccgg aacctgaact caccgcacag gcgttctaca taaaacgctt
     9601 acgcttcatt gttgactcgg agtcgacttc agataaatca cgctttaccc ttgatggagc
     9661 ctgtacatag atttgtgtaa ttgcctgatt ttgatatgtt caattcaaca tcaaatgaag
     9721 gttaaattat ggacgacaaa caattgcagg ctcaggctgc gttcagcaaa gcatcgcaac
     9781 cggcgataga tgcttcatta aatttaagat tcagcttcct cttcagccac ccgtacgcca
     9841 atcttcaaca cttcattatc ttctttctcg gccaccgtcc agatcatccc ggcaaactct
     9901 acctggtcac cgacaaccgg tgccgtgcct aacaactgct ggacaatttc acccagcgtt
     9961 tgctgcttat cacgatattc tcgcccgtct tccaggccat atatcagcgc aacatcagca
    10021 tatttggcgc tggcttcgag aatgaagtca ccaaagaagc gttgatccag cgcgaccggc
    10081 ggcgactggc tgaacagttt accgagcgcc ggaagatcgc gttcccgacc aattacacac
    10141 aacacatcgc cttcgcgcag tcgggtgctg ccggtaggat gaagcaactg gttatcacga
    10201 aacagtgccg caatacgcgt ctcttttggc atatgcaaat cgcgcagtgc cgcgcccacg
    10261 caccatttat cggcactcaa ttgataaaca aactgctccc acggattttc cggatgaata
    10321 tccaggccaa cgcgtgacac cggacgtccc actggcggaa ccaccacttt ggctttttta
    10381 gccgcccacg agagtgatgt tccctgcaag agcagtgaaa ccagaaccac aaagaaggcg
    10441 acattaaaga acagacgtgc attctccagc cccgccatca tcgggaacac ggccaggatg
    10501 attggcaccg cgccgcgtaa tcctacccag ctgataaaca cgcgctcacg cagattgaaa
    10561 ccgcggaagg ggagcaatcc agcaaatacc gaaaggggac gggcgaagaa tatcatccat
    10621 gccgacaaaa ttagcgccgg aatggcaata ggcagcagat cgcttgggtt aaccaatagc
    10681 cccagcacca ggaacatggc gatttgcgcc agccaggcga ggccgtcgaa attttgcagg
    10741 atgccgtagc ggttgcgaat cggccgatta cccagcagaa aaccgcacag ataaacagcc
    10801 agaataccgc tgccttccag cgccgtggtt aaagcaaaaa ttaaaatacc gccgcttaat
    10861 gccagcaatg gatataatcc ggcaggcagg gcgatgcgat taatcatttg cagcagtaaa
    10921 taaccaccgc caagtccgat gacaatcccg aggccaaatt gctgcagaat atcgaccacg
    10981 aacatccaac tgacactgct ttcatgttgc tggatcatcg caatcagggt aatcgtcaga
    11041 aagaccgcca ttggatcatt actgccggat tcaatttcca gcgtcgagcc aacacgttcg
    11101 ttaagcccct taccacccag cagagaaaag accgctgcag catcggtaga gccgacgata
    11161 gcgccgatta ataagccttc aatcaaatca agattaaaca gccacgccgc catcatgccg
    11221 gttaaaccag aggtgataag cacgcccagc gtcgccagcg acagtgccgg tcctaacgcc
    11281 acacgaaagg aactggcctg agtgcgcatc ccgccgtcga gcaaaataat cgccagtgcc
    11341 aggttactta ccatgtaggc gaaggggtaa ttatcaaacg ggataccgcc gacgccatcg
    11401 actcctgcca gcatgccgat cgccagaaaa ataaccagaa taggaatgcc aagacgggaa
    11461 gaaaatgaac taagtaatat actgctggtg acaaggatgg atcctaagat gaaaaggcta
    11521 attattgttg tggcatccaa cggtcggtta ctcctgatta cgctgtctct tatataaacc
    11581 ctaccatatt agcggcagag acagcgtttt acttagccct gaagcgtgat ttttttacat
    11641 tttaagaaca ggatgaccgc taatcgtcaa ctgagtgcct tcccgggtgt tattcagaat
    11701 ggcatgtgcg cccaacggca gcgtaaccgt gcgttgttca tgaccaaaat cgaggccggt
    11761 aatgagtgga atcgacaggc gggaacgcaa aaacgcggac actgactcca ggttgtaacc
    11821 tgcgtcataa tcattgggcg tgctgccgct aaagctaccg agaataatcg ccttctgacg
    11881 tggcaaaatt cccgcatgat agagctgcaa cagcatacgt tcgacgcgga aagggtgctc
    11941 gttaatatct tccagtacca ggataccgtt ctcaattttt ggcatccacg gtgtaccaat
    12001 cagtgaaatc agcatcgcaa gattgcctcc ccacaacgtg ccttcggtct gacatgtcgg
    12061 gccttcccct tgccattcaa tggtgaaggt tttattgcgt aacgccagcc agaaatggtg
    12121 ctcggtaaag gcgttcagtt catccgcacc aaagtttgct accagcatgg ggccgctaaa
    12181 ggtgatgaca ttgccttgcg ccagaagacc gcactgaatg gcggtaaaat cgctatgtcc
    12241 gcaaatgagt aacgggtcat gttgttggcg agccaccagt gcctgccagt caatatctgc
    12301 cagtaaacga ctggcaccgt aaccgccgcg tacagccagc acgatggtgt tgggtgtagt
    12361 cagtctggca agggaattga gatcttccag acgctccgtt tccgtacccg caaaacgctc
    12421 acaacgacgg gcaataacct cgacgttatt gacttgatgc cccgcgtcgg taaggcgctg
    12481 gataccacgc agcgcggcgt gctgtttaat gctgtaaccc gatggggcaa ttaagtgaaa
    12541 cagagacatg gcaattcctt gctgacaaca gaaacgaaat gtatatcatg ccgcttaggt
    12601 gtgccgttgt cacctcaacg gcgattccag gctataagga tagaagaagt gaaattgaga
    12661 tggtttgcct ttttgattgt gttattagcg ggttgttcat caaagcatga ctatacgaac
    12721 ccgccgtgga acgcgaaagt tccggtgcaa cgtgcgatgc agtggatgcc aataagccag
    12781 aaagccggtg cagcctgggg cgtcgatcca caattgatca cggcgattat cgctatcgaa
    12841 tcgggtggta atccgaacgc ggtgagtaaa tcgaatgcca ttggtttgat gcagttaaaa
    12901 gcttcaacct ccggacgtga tgtttatcgc cgtatgggct ggagtggtga gccgacgacc
    12961 agcgagctga aaaatccaga gcgtaatatt tcaatggggg cggcttacct gaatattctg
    13021 gaaaccggcc cgctggcagg cattgaagat ccgaaggtac tgcaatatgc gctggtggtg
    13081 tcatacgcta acggggcagg tgcgctgcta cggactttct cgtcagatcg gaaaaaggcg
    13141 atcagcaaaa tcaacgattt ggatgctgac gagttcctcg atcacgtagc gcgcaatcat
    13201 cctgcgccgc aggctccgcg ctatatctac aaacttgagc aggcactgga cgcgatgtaa
    13261 atcagtcgcg tactttgtcc gctttctccc gggcttcccg ctcgagagag aaaataatcc
    13321 gttgtaattg ccgctccacc gtcgggctga cgttaagaaa acggaagctc agacggggag
    13381 tggtgatggt ttcattcttg ccatcaatca ctttgcgctc gctgatggag attaactggg
    13441 cgtcaaagtg aaaaacaccc cattgcccca tgttgacttc aatctgagcg aagcgcatgc
    13501 cttcttgtaa ttcggcaggc tttgctgttt ccagtaatgc gcccatgccg cctaacgaca
    13561 aatcatacag gcggaaacgt aacgtactgt tatccgccag tttggtctgg caaaaataag
    13621 gcggatggag tggggcggag atgcggaaat atcggcgtcg ttgtacaaac cataaggtgg
    13681 gaggcggtac ggtaataaat gccggaagct gcaagtattc actctgctgt agttgttcaa
    13741 cagtaaactc gactttcgca ccctgagttt cggcggtaat ggtaatatgc tgcgccttta
    13801 gcacggcgat gttgtcttcg gcttgactgc cgaaatccag caccagttta tccggggtta
    13861 ttgccagtat tttgctgatc agctgcccgc cattccaact gagacgcaaa ggaattgcgg
    13921 ctttgtgcaa atcgcgtaac acgcccagga cggctaacgg attttgtttc aggaactgct
    13981 catggtaatg actcacgcgg aaaaactcct gactgacaaa ctgtctttgg tttatcggtc
    14041 acagttaaca aaacttaata caaatgcgtg aatatttttt acatgttgtt cttaaatcag
    14101 ccgacatacg cccagcatcg atcccgccct tgcctatact tagagcgttg acgtaagcat
    14161 ttcttgcgtc cgattcatcg aaacgagggc ttgaacatgg gaattattgc ctggattatt
    14221 tttggcctga tagccggcat tatcgccaag ctaatcatgc cggggcgtga tggtggtgga
    14281 tttttccaga cctgtattct cgggatagtc ggtgcggtgg tcggcggctg gctggcgacc
    14341 atgtttggca ttggcggctc catcagcggt tttaatttgc acagcttcct ggtggcggtg
    14401 gtgggagcta ttctcgttct gggcttattc cgcctgctgc gaagagaata agattttcat
    14461 aaggcggata gcgatacaga tgccgctatc cgcttttcac atcagaacgt atattcgaca
    14521 cctgctttac gggtgaaaaa aatcaatata gcacttcgat atccgataaa gtcggttccc
    14581 ggtaacaaca acagaatcct tttttaatta ttgtttcgtt gtttttggtg tgattcagaa
    14641 aataaatatt ttagacagta gccagtcagg cgttaaaaat ggggcggata gtgaggcggt
    14701 tttggctgcg cttattgaac atttagaatt gatcaatcct tctggcagat taagccaaaa
    14761 tacccgttcg gcaatgcttc agttacgcga agaggaatgg tctgaattct ttttttggtt
    14821 gctaaattct ctggagtgtc tggattatgt gattattaat ttaactccag aaagcaaaaa
    14881 aacgttgatg agtgaacacc gtaacaatat tcaggtagca atcgatgctt tatataggca
    14941 acgtcggtgt aaatcaccgg gggatgatag tgaaacactt acccgtagaa atgacgctat
    15001 cttcggcaac cacgtatggc aaacatttgc gcaatacttc ccgccaggac ttgaaaaacc
    15061 ggctgtgtag aaaacaccat agctatggtg ttatctgccg acaacgccat ccggagaagc
    15121 cgaatgacgt tgtcggagta agcgctggtt aaggtgtggg ttgtgcctct ttggttgagg
    15181 gttgcgtcgt tgctgactta acggtcggac gcgtcgccgg aacattgtca cacggttgct
    15241 ctttcgggca gatcaaatcc agcattttca gcgtcacgcc attggtccag ccaaagccat
    15301 cctgtaatgg atattcgcca cctctttttg cccgtagttt tgtaatcctt ctgtcgcgac
    15361 ccactgtaac ggtgcccagc catttggcgc atcccattgt tgcccacttt tcaccgacgt
    15421 ggtgttcagg ccgccgggtt gcagcagatg tgttttcgtc gccgtcgcca ttttgttggc
    15481 gcgatctttc gctgccgcat tgacgtacag cgggaacagg gcggccgcgg ttaactgatt
    15541 gcgcacttta tgacttttca ggtcgtaatc ggcataccag ccttgttgat cgttccacag
    15601 gtatttttcg attccttttt gacgggcatt cgccagcgtt tcgtactggt ttgccatcgc
    15661 gttatctcct gcagctttgc tggcgcgggc gaggattttt tccattttaa acatcaggct
    15721 gttcagatcg accggtacga tgctggtggt gcgtaaggta tttaactgct gcgggttgtc
    15781 catccagcgc gagctgaaat cccagccaga cgcagcggca gagcgcaggt cgcggtaaat
    15841 ttcagtggca ggtcgattcg gattgctttt ggcggtggca atatcttcca cccatgactc
    15901 tggtcgtggc gtatcgcgat cgtcccagta gcggttgaga agggtaccat cctgaagttt
    15961 gacaacgcgt ttttcctgtt gtccggcttg caggttttca acaccgtcca tccagtaagc
    16021 atattctttt tgcatttgcg gcaggtattg cttcaacgcg gcatcgcctt catgctgcgc
    16081 cagtaactct accatcaggg caaagaaggg cggttgcgag cggcttaaat agtaactgcg
    16141 gttgccgttg ggaatatgac cgtaagtgtc tatttcatga gcaaaattgg ccaccatatc
    16201 cgcgacttta tcccagtgac cgctttcggc aagtcctaac atggtgaagt aactgtccca
    16261 gtaatatacc tcgcgaaaac gtccgcccgg cacgacataa ggttccggca gcggtaacag
    16321 agaatcccat ttttcggtgt tttcggtaga acgcgttaat accggccaaa gtccgtcaat
    16381 atgttcgcgc agtgactgcc cctctggcgg aacatatttc tcgccttctt tcggcagggt
    16441 gaaattgacg ttaacgaaat ggcgcagatc aaatccgctc tggttttgct gcatccgata
    16501 atcagcggat cgctgttcgg cacggcatcg gcaaaggttt tttggtccgg aaaaagtttg
    16561 gcgttttgca catcattaaa cagcggccct aataaaatat caggcggctg tggtgttacc
    16621 ggtgtttctt ctgcctgcac cgatagcgca gcgaaacaca aaaagataca ggct
//
LOCUS       DAZPEB010000079        16669 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZPEB010000079 DAZPEB010000000
VERSION     DAZPEB010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16669
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.080"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..94)
                     /locus_tag="ACHURJ_002232"
     CDS             complement(<1..94)
                     /locus_tag="ACHURJ_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR1281171.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVSWW"
     gene            complement(499..1599)
                     /gene="chaA"
                     /locus_tag="ACHURJ_002233"
     CDS             complement(499..1599)
                     /gene="chaA"
                     /locus_tag="ACHURJ_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFR1281172.1"
                     /translation="MSNAQEAVKTRHKEISLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNKLQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            1869..2099
                     /gene="chaB"
                     /locus_tag="ACHURJ_002234"
     CDS             1869..2099
                     /gene="chaB"
                     /locus_tag="ACHURJ_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFR1281173.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKDDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            2257..2952
                     /gene="chaC"
                     /locus_tag="ACHURJ_002235"
     CDS             2257..2952
                     /gene="chaC"
                     /locus_tag="ACHURJ_002235"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFR1281174.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGCTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(2996..3349)
                     /gene="ychN"
                     /locus_tag="ACHURJ_002236"
     CDS             complement(2996..3349)
                     /gene="ychN"
                     /locus_tag="ACHURJ_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFR1281175.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            3533..4927
                     /gene="ychO"
                     /locus_tag="ACHURJ_002237"
                     /gene_synonym="iatA"
     CDS             3533..4927
                     /gene="ychO"
                     /locus_tag="ACHURJ_002237"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFR1281176.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYFTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLLANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEYNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(4928..5578)
                     /gene="narL"
                     /locus_tag="ACHURJ_002238"
     CDS             complement(4928..5578)
                     /gene="narL"
                     /locus_tag="ACHURJ_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFR1281177.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(5571..7367)
                     /gene="narX"
                     /locus_tag="ACHURJ_002239"
     CDS             complement(5571..7367)
                     /gene="narX"
                     /locus_tag="ACHURJ_002239"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFR1281178.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDEQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPASSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            7706..9097
                     /gene="narK"
                     /locus_tag="ACHURJ_002240"
     CDS             7706..9097
                     /gene="narK"
                     /locus_tag="ACHURJ_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFR1281179.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            9613..13335
                     /locus_tag="ACHURJ_002241"
     CDS             9613..13335
                     /locus_tag="ACHURJ_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFR1281180.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERAYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNQLMLTLGRGGPVVWLSEADAKELGIADNDWIEVYNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITGQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGND"
     gene            13353..14891
                     /gene="narH"
                     /locus_tag="ACHURJ_002242"
     CDS             13353..14891
                     /gene="narH"
                     /locus_tag="ACHURJ_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFR1281181.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            14888..15598
                     /gene="narJ"
                     /locus_tag="ACHURJ_002243"
     CDS             14888..15598
                     /gene="narJ"
                     /locus_tag="ACHURJ_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFR1281182.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEVIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            15598..16275
                     /gene="narI"
                     /locus_tag="ACHURJ_002244"
     CDS             15598..16275
                     /gene="narI"
                     /locus_tag="ACHURJ_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFR1281183.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFAGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(16458..16589)
                     /locus_tag="ACHURJ_002245"
     ncRNA           complement(16458..16589)
                     /ncRNA_class="other"
                     /locus_tag="ACHURJ_002245"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
BASE COUNT         3993 a         4173 c         4471 g         4032 t
ORIGIN      
        1 gccaccagct gacaatcgct gcggtaataa tccccgccag gatcggtgct gccaggtcgt
       61 gccagaaagt catggcaaac tgcgcgagcg tcatatagcc gccttgttgt aatgacaaca
      121 ttttgcggct attcttgaag tgtctggttt caagattagc ccccgttttg ttgtcaggtt
      181 ttacctctca acgtgcgggg gttttctctt tccagcaacc aatgccacca gggataaagc
      241 ccccgcaaca ttgcgcctca ccggataatt ccggcttggt gtggatacta cgtctcaatt
      301 catcttcact tcatccctga aatgtttgca ataaagagta cattccggct tttcaacagc
      361 tgttgcagtg gtttcgtgca ggttgtggta caggctcgca attctgatta acgagctgtc
      421 caggtagtat gaagaaatac aataagcagg aatttatctt atctcccccg atttccgtca
      481 tcggaggaga tataactgtc aggcaaatat cgtcatcaaa taggcggcaa acagtgccag
      541 atgcgctgcg ccattgagca cgttagtacg tccggtggag aaggagatat ggcacagcac
      601 taaagaggcc accatcacca ccatttctgg cgcaccaagt gcaaactgta atttgttacc
      661 cgtcataaag gcaattagcg tgacgacagg tacggtaagt gaaatggttg ctaacactga
      721 accaaagaac agattcatcg cgcgctggac ctggttgttc aacactgctt ttaacgcacc
      781 taaaccttcc ggcgacagaa tcaacagagc caccaggaag ccagtaaagg cgacaggggc
      841 attcatgctg tcgagcaatg tctccagcgg gctggcattc attttggtga ctgcaataac
      901 ggcaatcaga tggataatca accagatagc atgccacacg ctgctatggg cagacggttt
      961 accgtgatgc gggtcgtcgt catcactgtc atcttcgtgc tcgtagacaa acaaactttg
     1021 atgcgttttg gtctggatca gcaaaaatac gccatacatt gccgcagaaa ttaatgctac
     1081 cagtaacgcc tgaccggttg aaaaattcgc cgcaggcaga gccattggaa ataccagtac
     1141 gattatcgcc agggggaaca gcgcaattaa atactgcttg ataccaaaca gattcatata
     1201 ttgggtggca aacttacgac cgcccaacaa taatgaaaag ccaaccagcc caccggtaac
     1261 aatcataatg attgaataga gcgtatcacg cattagcgtt ggcgcggcgt cgccggttgc
     1321 cattaaagct gaaatcaaac tgacttcaag aataaccact gaaaggctaa gaataagcga
     1381 accgtaaggt tctcccaagc gatgggctaa tacgtccgca tgacggacaa cactaaaggc
     1441 gctacttaaa ataccaataa gcgcaagaag attgatggca atgaccactg gtagtgtctg
     1501 gctgcttccc cacaggaaca gcactaccag cgccagaacc gggaaaataa gcgaaatctc
     1561 cttgtggcgg gtttttaccg cctcttgagc atttgacatt atggttatcc ctttgcagat
     1621 gaatttatcg aaaatgtaaa aaataggtag gaaaaataac agaaagtgtc tggatatcgg
     1681 taacatttta cgaattttta ccccactgtc gattttttac tcattgggac ataaaaataa
     1741 gtattacgtt tagacaatgt ttgttttagc gtttcttaag aacagtctga catgaaaatt
     1801 cttatgtttt ggcaagagta gatattgttg accacactta atgttcaact ttgtaaaagg
     1861 agtcaacgat gccgtataaa acgaaaagcg atctgccgga aagcgtaaag cacgttctac
     1921 cgtctcatgc ccaggatatc tataaagaag cgttcaacag cgcatgggat caatataaag
     1981 ataaagacga tcggcgtgat gacgccagtc gcgaagaaac cgcgcataaa gtggcctggg
     2041 ctgctgtgaa gcatgaatat gccaaagggg atgatgataa atggcataaa aaatcgtaaa
     2101 accggtagct tagttaaagc tattcgtgcg gtgttgcctt gcaagtggtc cgtggattgc
     2161 atattgtccc gttagtggtt tcaaaatgag cagtaaaaat gtccggaaga caccaaaaag
     2221 ttgtcgcagg gaagtatgca gtggcggagg tgtaaggtga taacgcgtga tttcttgatg
     2281 aatgccgatt gtaaaacggc atttggtgcc attgaagaat cactcttatg gtcagcagag
     2341 caacgagcgg cttcgctggc ggcgacgctg gcttgtcgac ctgatgaggg accggtgtgg
     2401 atcttcggtt atggatcgtt gatgtggaat ccggcactgg agtttaccga gtcgtgcacc
     2461 ggtacactgg ttggatggca tcgcgcgttt tgcctgcgcc tgaccgccgg gcgcgggact
     2521 gcgcaccagc cgggacggat gcttgcactg aaagagggcg gacgcactac aggcgtcgcc
     2581 tatcgactgc cagaagagac gctggagcag gaactaaccc tgttgtggaa gcgcgagatg
     2641 attaccggct gttatctgcc aacctggtgt cagcttgatc ttgatgatgg atgcacagta
     2701 aacgccattg tgtttattat ggacccgcga catccagaat atgaatctga cactcgcgct
     2761 caggtcatcg cgccgttgat tgcggcggcg agcggtccgc tgggaaccaa cgcacaatac
     2821 ctgttttcac tggaacagga gcttatcaaa ctgggaatgc aggacgatgg gctgaatgat
     2881 ttgctggtat cggtaaaaaa actgctggcg gagaattatc cggatggtgt gttacggccg
     2941 ggattcgcct gagtaaactt cccgcatagt ggggcgtcag acgccccctc aaacattaaa
     3001 atgtgagcac tttatcggct gacagcgtcc attgcgccag ttccacaaga gtaccgattt
     3061 ccaccccatc aatcagagga agtgtactaa tcccgcgccc gtcggtacag gttttgcaca
     3121 atttcaccgg tacattctga gcggtaagga tctccagcat ttgctgaatg ttgtagcctt
     3181 cccctggttt ttgcccgcgc aacccggctg tgaccgcatc agacatgagg aacagacgca
     3241 gatccagatt gctctcctgc tctcgtaacg caatggccag ccgcaagctg ttaaacaagg
     3301 attcgctccc gtaaggtgcg ccattggcaa cgatcacgat tttttgcatt atttactcct
     3361 gtattcaggg aattagacac tcatcttcta tcttactgct tctgcagcgt ctgaccaatc
     3421 ggtcacattt ttaaggattt tcctgaaagc gcgagaaaat acgacaaaag ttgccagtaa
     3481 tcgttattct ttaaggctat ggttttcatt attaccggaa gttaccgacg ttttgagccg
     3541 tttcgttcct cgcattatcc cgttttattt actcttgctt gtagcaggcg gtacagctaa
     3601 cgcacaatct accttcgagc aaaaagcggc aaatcccttt gataataaca atgatggtct
     3661 gccggattta ggcatggctc ccgaaaatca tgatggggaa aaacactttg ctgaaattgt
     3721 gaaagatttc ggcgaaacca gtatgaatga taacgggctg gatactggcg agcaggcaaa
     3781 agctttcgca ttgggaaaag tccgcgacgc gcttagtcaa caggttaatc agcacgtaga
     3841 gtcctggcta tcaccgtggg gaaatgccag tgttgacgtc aaagtggata acgaaggaca
     3901 tttcaccggc agtcgtggaa gctggtttgt gccgttacaa gataatgatc gttatttcac
     3961 ctggagccag cttggtctta ctcagcagga taatgggttg gtgagcaatg tgggcgttgg
     4021 gcaacgctgg gcgcgcggca actggctggt gggttataac actttttatg acaacttgct
     4081 ggacgaaaat cttcagcgag cgggctttgg tgccgaagcg tggggcgaat atttgcgatt
     4141 attggcaaac ttttatcagc cgtttgctgc atggcatgaa cagacagcca cgcaggaaca
     4201 acggatggcg cgcgggtacg acctgacagc tcggatgcgc atgccgttct atcaacacct
     4261 caataccagt gtcagcctag aacagtattt tggtgatcgt gttgatttgt ttaactctgg
     4321 tacgggttat cacaatcccg tcgcgttgag tctgggatta aattacaccc ctgtgccatt
     4381 agtcactgtg acggcccagc ataaacaggg tgaaagtggc gagaatcaaa ataacctcgg
     4441 gctgaatctt aattaccgct ttggtgtacc gctcaaaaaa caactttctg cgggcgaggt
     4501 tgccgaaagt cagtcgttac gtggtagtcg ctatgacaat ccgcagcgaa ataatctacc
     4561 gactcttgag taccgacagc gaaaaacgtt aacggtgttt ctggcgacac cgccgtggga
     4621 tctaaaacct ggcgaaacag tgccgctgaa attacaaatc cgcagtcgtt acggtattcg
     4681 gcaactgatt tggcagggcg atacgcagat attaagtttg acgccgggcg cacaagccaa
     4741 cagcgcggag ggctggacgc tgatcatgcc tgactggcag aacggggaag gggcaagcaa
     4801 tcactggcga ttgtcggtgg tggtggaata taaccagggg cagcgtgtct cctccaatga
     4861 gatcacgcta acgcttgtcg aaccgttcga tgcattgtca aacgacgaac tgcgctggga
     4921 accgtaatca gaaaatgcgc tcctgatgca cccataccgc tgcttccacg cgagacttga
     4981 gcttcatttt cttcagcatg tgcttgacgt gcacttttac tgtgctttcg gtgatatcca
     5041 ggcggcgggc aatcatcttg ttcggcaaac cctgggcaat cagcttgaga atatcgcgct
     5101 cgcgtggggt taactggtta acatcgcgct cagtagtggc acggttagcg cgcaagctgg
     5161 cggccagaac aggcgttaat gcttcgctta ataccatttc gccagcagct gcctgatgca
     5221 atgctttcag cagatcttcc ggttccatat cttttaacag ataaccatcc gcgccgcgtt
     5281 tcagtgcggt gaccacatct tcttcatggt tagagacgct gaataccaca atgcgccctg
     5341 agagggactt ttcgcgcagt ttatccagcg tttccagacc gttcatgccg ggcatattga
     5401 gatctaacag gatcagatcg ggatcaagag actccgccag ttcaataccc tgttcgccat
     5461 tactcgcttc gccaaccacg gtgatatctg gtgccatact gataagctgt tttacgccag
     5521 ttcgcagcat cgggtgatcg tcgatcagca ggatagtagc cggttcctga ttactcatgg
     5581 gtatctcctt ggacgtctgt gaaagttttt tcgggaataa aggtgaccac cacttcggtg
     5641 ccacctgatt cacgacggcg gacgcggcaa tcgcctcgta aactttgcgc acggtcgcgc
     5701 attattatca tgccgtagtg attgctgcgg atggcatttt caggcacgcc gcagccgtta
     5761 tcttggacgg tcagtttgac ctgattatcg ttttgcgcca ccgtcaccac gacctcactc
     5821 gcttgcgaat gtttgagggc gttacttaat gcctcacggg caatttgcaa caagtggatt
     5881 gcctgatgtg aaggcaccag acgaggcggc aattgataat ccagcttcac cgggaagcca
     5941 aatttggcgc tgtactcttc gcaactcgcc tccagcgccg gacgtaatcc aggctcggtg
     6001 agctgcaagc ggaatgtggt gagcaattca cgcaactgcg cccaggatgc attcagttcg
     6061 ttacggatct gacttaacag ttcgcggctg cttgctggca gcgcatcgcc ctgcatctgt
     6121 aaacaactca cctgcatctt catgcaagag agagattggg caatagaatc atgcagttcg
     6181 cgcgcaatgg tggcacgctc ttccatcacg atcaactgtt gctgacgttc ctgatggcga
     6241 tccagcgcca gcgtggcggt gagttgttca accagggtat ccaccagttg ttgttgatca
     6301 tggctaagat gacgcccctg cggcagggtc gccagcaaaa taccgtactg cgtatgagag
     6361 tcagccagcc gccacttcag ggttgtgccg cgatcaccaa cgggtaatac gccgcgcggg
     6421 cagagctggc agcctttatc atcacaagtc atatctggct ggcaggtaaa ctcctgatga
     6481 ttctcttcat catcagtgtc atacacccgc agttcgatat cacgtagcag ggttaaattc
     6541 tgtaagccgt tgagtacagg tgacaggcgt tcacacagcg gggcgcggga atgcaaacgg
     6601 cggttagcct gccataaaaa agagaggatc tgatttttat gctccagccc ggcggttttc
     6661 tcctgaaccc gctgctcaag tacggcataa ctttcggcca gttctgcaga catattgttc
     6721 aatgcagttc caagcatcgc catttcgttg cgcccgctga tgttcgcgcg ttgggtaaaa
     6781 tcgcgatgac tgacggcact cgccattgcc agcagttgcc gccacggttg tagcagtcgc
     6841 gcccgcaacc agataatagt gaacaccagt aaaagtgcca taaataccgc cattacccga
     6901 tggaccagta ccactgtctc aatgcgcatt tccgtggtgc ggtcaaaacc agataccagc
     6961 tgatcaagcc cggcaacaaa ctggctgaca tccgctgaca ccgtttcgcg gttttgtgca
     7021 cgcatcagtg cagggatcag ttcattacgc cagtaatctt gtaaaccctg taattgcgcc
     7081 agttgttcgt ctcgttctgc tgctcgagtt aactcggcgc taaatgccgt ttgttccatc
     7141 tctttaatta agggcttgtc tttctcactt aatggcactg ccgccaacag acggtaactt
     7201 tgcatgcgca gcgatcccgc tttgttgatc gcatgggcgc tgccctgaac gccttgcacc
     7261 agccagccag aaaccgccat ccctgccagt ccaatagcag tagaaagcaa cacaataagc
     7321 gcaacctgat taaccagggt gagcggagag agacaacgtt taagcatgta aacctcttcc
     7381 ttcaggcttt aaatgagcaa taaccttaat gaatgtgacg atacattctg gaatggcagt
     7441 attctcggct attggctgaa gtatacccat acccggaaag agttactcct tatttgccgt
     7501 gtggttagtc gctttacatc ggtaagggta gggattttac agcaccgtga aaaatctcat
     7561 aatttttatg aaatcactgt actcactatg ggtattgata aatatcaatg atagataaag
     7621 ttatcttatc gtttgattta catcaaattg cctttagcta cagacactaa ggtggcagac
     7681 atcgaaacga gtatcagagg tgtctatgag tcactcatcc gcccccgaaa gggctactgg
     7741 agctgtcatt acagattggc gaccggaaga tcctgcgttc tggcaacaac gcggtcaacg
     7801 tattgccagc cgtaacctgt ggatttccgt tccctgtctg ctgctggcgt tttgcgtatg
     7861 gatgttgttc agcgctgttg cggtgaactt accgaaagtc ggttttaatt ttacgaccga
     7921 tcagctattt atgttgactg cgctgccttc ggtttctggc gcgttattac gtgttccata
     7981 ctcctttatg gttcctatct tcggtggtcg tcgctggacg gcgttcagca ccggtattct
     8041 gattattcct tgcgtctggc tgggttttgc cgtgcaggat acctccacgc cttatagcgt
     8101 cttcatcatc atctctctgc tatgcggctt tgctggcgcg aacttcgcat ccagtatggc
     8161 aaacatcagc ttcttctttc cgaaacagaa gcagggtggc gcgctgggtc tgaatggtgg
     8221 tctgggaaac atgggcgtca gcgtcatgca gttggttgct ccgctggtgg tatcactgtc
     8281 gattttcgca gtatttggta gccagggcgt caaacagccg gatgggactg agctgtatct
     8341 ggcgaatgcg tcctggatat gggtgccgtt ccttgccatc ttcaccattg cggcgtggtt
     8401 tggcatgaac gatcttgcta cctcgaaagc ctccatcaag gagcagttgc cggtactcaa
     8461 acggggtcat ctgtggatta tgagcctgct gtatctggca accttcggct ccttcatcgg
     8521 cttctccgcg ggctttgcaa tgctgtcaaa aacgcagttc ccggatgttc agattctgca
     8581 atacgctttc ttcgggccgt ttattggtgc gctggcgcgt tctgcaggtg gtgcattatc
     8641 tgaccgtctg ggcggaactc gtgtcacgct ggtgaacttt attctgatgg cgattttcag
     8701 cggcctgctg ttcctgacct taccgactga cgggcagggc ggaagcttca tggcgttctt
     8761 cgcggtcttc ctggcgctgt tcctgacagc tgggctgggt agtggttcca ctttccagat
     8821 gatttcagtg atcttccgta aactgacaat ggatcgcgtg aaagcagaag ggggttctga
     8881 cgaacgtgcg atgcgtgaag cggcaaccga cacggcggcg gcgctgggtt tcatctctgc
     8941 gattggcgcg attggtggct tctttatccc gaaagcgttt ggtagctcgc tggcattaac
     9001 gggttcgcca gtcggcgcaa tgaaggtatt tttgattttc tatatcgcct gcgtagtgat
     9061 tacctgggcg gtatatggtc ggcattctaa aaaataaacc gttactcgtc atactccggg
     9121 ttacatgtgc tgcggctgcg ttcattcacc ccagtcactt actttagtaa gctcctggga
     9181 ttcattcact tgccgccttc ctgtaaaccg aattatatag agtaaaatat ttgattatcc
     9241 tttgcgcggc atgatgtcgc gcttttttta agcgttattt agttacaaca tactaatgtt
     9301 atatggttta tttcgccgga tttcattaag agccattaat atgttcccca tggggaatac
     9361 tccttaatac ccatctgcat aaaaatctta atagtttaaa taactacagg tataaaacgt
     9421 cttaatttac agtctgttat gtggtggctg ttgattatcc taaaggggta tcttaggaat
     9481 ttactttatt tttcatcccc atcactcttg atcgttatca attcccacgc tgtttcagag
     9541 cgttaccttg cccttaaaca ttagcaatgt cgatttatca gagggccgac aggctcccac
     9601 aggagaaaac cgatgagtaa attcctggac cggtttcgct acttcaaaca gaagggtgaa
     9661 acctttgccg atgggcatgg ccagcttctc aataccaacc gtgactggga ggatggatat
     9721 cgccagcgtt ggcagcatga caaaatcgtc cgctctaccc acggggtaaa ctgcaccggc
     9781 tcctgcagct ggaaaatcta cgtcaaaaac ggtctggtca cctgggaaac ccagcagact
     9841 gactatccgc gtacccgtcc ggatctgcca aaccatgaac ctcgcggctg cccgcgcggt
     9901 gccagctact cctggtatct ttacagtgcc aaccgcctga aatacccgat gatgcgcaaa
     9961 cgcctgatga aaatgtggcg tgaagcgaag gcgctgcata gcgatccggt tgaggcatgg
    10021 gcttctatca ttgaagacgc cgataaagcg aaaagcttta agcaggcgcg tggacgcggt
    10081 ggatttgttc gttcttcctg gcaggaggtg aacgaactga tcgccgcatc taacgtttac
    10141 accatcaaaa actacggccc ggaccgtgtt gctggtttct cgccaattcc ggcaatgtcg
    10201 atggtttctt acgcatcggg tgcacgctat ctctcgctga ttggcggtac ttgcttaagc
    10261 ttctacgact ggtactgcga cctgcctcct gcgtctccgc aaacctgggg cgagcaaact
    10321 gacgtaccgg aatctgctga ctggtataac tccagctaca tcatcgcctg gggttcaaac
    10381 gtgccgcaga cgcgtacccc ggatgctcac ttctttactg aagtgcgtta caaaggcacc
    10441 aaaactgttg ccgtcacacc agactacgct gaaatcgcca aactgtgcga tctgtggctg
    10501 gcaccgaaac agggcaccga tgcggcaatg gcgctggcga tgggccacgt aatgctgcgt
    10561 gaattccacc tcgacaaccc aagccagtat ttcaccgact atgtgcgtcg ctacaccgac
    10621 atgccgatgc tggtgatgct ggaagaacgc gacggttact acgctgcagg tcgtatgctg
    10681 cgcgctgctg atctggttga tgcgctgggc caggaaaaca atccggaatg gaaaactgtc
    10741 gcctttaata ccaatggcga aatggttgcg ccgaacggtt ctattggctt ccgctggggc
    10801 gagaagggca aatggaatct tgagcagcgc gacggcaaaa ctggcgaaga aaccgagctg
    10861 caactgagcc tgctgggtag ccaagatgag atcgctgagg taggcttccc gtactttggt
    10921 ggcgacggca cggaacactt caacaaagtg gaactggaaa acgtgctgct gcacaaactg
    10981 ccggtgaaac gcctgcaact ggctgatggc agcaccgccc tggtgaccac cgtttatgat
    11041 ctgacgctgg caaactacgg tctggaacgt ggcctgaacg acgttaactg tgcaaccagc
    11101 tatgacgatg tgaaagctta taccccggcc tgggccgagc agattaccgg cgtttctcgc
    11161 agccagatta ttcgcatcgc ccgtgaattt gccgataacg ctgataaaac gcacggtcgt
    11221 tcgatgatta tcgtcggtgc ggggctgaac cactggtatc acctcgatat gaactatcgt
    11281 ggtctgatca acatgctgat tttctgcggc tgtgtcggtc agagcggggg cggctgggcg
    11341 cactatgtag gtcaggaaaa actgcgtccg caaaccggct ggcagccgct ggcgtttgcc
    11401 cttgactggc agcgtccggc gcgtcacatg aacagcactt cttatttcta taaccactcc
    11461 agccagtggc gttatgaaac cgtcacggcg gaagagttgc tgtcaccgat ggcggacaaa
    11521 tcccgctata ccggacactt gatcgacttt aacgtccgtg cggaacgcat gggctggctg
    11581 ccgtctgcac cgcagttagg cactaacccg ctgactatcg ctggcgaagc ggaaaaagcc
    11641 gggatgaatc cggtggacta tacggtgaaa tccctgaaag agggttccat ccgttttgcg
    11701 gcagaacaac cagaaaacgg taaaaaccac ccgcgcaacc tgttcatctg gcgttctaac
    11761 ctgctcggtt cttccggtaa aggtcatgag tttatgctca agtacctgct ggggacggag
    11821 cacggtatcc agggtaaaga tctggggcaa cagggcggcg tgaagccgga agaagtggac
    11881 tggcaggaca atggtctgga aggcaagctg gatctggtgg ttacgctgga cttccgtctg
    11941 tcgagcacct gtctctattc cgacatcatt ttgccgacgg cgacctggta cgaaaaagac
    12001 gacatgaata cttcggatat gcatccgttt attcacccgc tgtctgcggc ggtcgatccg
    12061 gcctgggaag cgaaaagcga ctgggaaatc tacaaagcca tcgcgaagaa attctccgaa
    12121 gtgtgcgtcg gccatctggg taaagaaacc gacatcgtca cgctgcctat ccagcatgac
    12181 tctgccgctg aactggcgca gccgctggat gtgaaagact ggaaaaaagg cgagtgcgac
    12241 ctgatcccag gtaaaaccgc gccacacatt atggtcgtag agcgcgctta tccggcgact
    12301 tacgaacgct ttacctctat cggcccgctg atggagaaaa tcggtaatgg cggtaaaggg
    12361 attgcctgga acacccagag cgagatggat ctgctgcgta agctcaacta caccaaagcg
    12421 gaaggtccgg cgaaaggcca gccgatgctg aacaccgcaa ttgatgcggc agagatgatc
    12481 ctgacactgg caccggaaac caacggtcag gtagccgtga aagcctgggc tgccctgagc
    12541 gaatttaccg gtcgtgacca tacgcatctg gcgctgaata aagaagacga gaagatccgc
    12601 ttccgcgata ttcaggcaca gccgcgcaaa attatctcca gcccgacctg gtctggtctg
    12661 gaagatgaac acgtttctta caacgccggt tacaccaacg ttcacgagct gatcccatgg
    12721 cgtacgctct ctggtcgtca gcaactgtat caggatcacc agtggatgcg tgatttcggt
    12781 gaaagcctgc tggtttatcg tccgccgatc gacacccgtt cggtgaaaga agtgataggc
    12841 cagaaatcca acggcaaccc ggaaaaagcg ctcaacttcc tgacgccgca ccagaagtgg
    12901 gggatccact ccacctacag cgacaaccag ttgatgctga ccttaggtcg cggtggcccg
    12961 gtggtctggc tgagcgaagc cgatgccaaa gagctgggca ttgccgataa cgactggatt
    13021 gaagtttaca acagcaacgg tgcgctgact gcccgtgcgg ttgtcagcca gcgtgttccg
    13081 gcagggatga ccatgatgta ccacgcgcag gaacgtatcg ttaacctgcc gggttcggaa
    13141 attactggcc agcgtggggg gatccacaac tcggtcaccc gtatcacgcc gaaaccgacg
    13201 catatgattg gtggctatgc ccatctggca tacggcttta actactacgg caccgtaggt
    13261 tctaaccgcg atgagtttgt tgtagtgcgt aagatgaaga acattgactg gttagatggc
    13321 gaaggcaatg actaggtaca ggagagcgta aaatgaaaat tcgttcacaa gtcggcatgg
    13381 tgctgaatct cgataagtgc atcggctgcc acacctgttc agttacctgt aaaaacgtct
    13441 ggaccagccg tgaaggcgtg gagtacgcgt ggttcaacaa cgtggaaacc aagccgggtc
    13501 agggcttccc gactgactgg gaaaaccagg aaaaatacaa aggcggctgg atccgtaaaa
    13561 tcaacggcaa actgcagccg cgcatgggta accgtgccat gttgctgggt aaaatcttcg
    13621 ctaacccgca tctgccgggg atcgacgatt attacgagcc gttcgatttt gactatcaga
    13681 acctgcatac cgcgccggaa ggcagcaaat cgcagccgat tgcccgtccc cgttcgctga
    13741 ttaccgggga acggatggcg aaaatcgaaa aagggccgaa ctgggaagat gacctgggtg
    13801 gtgagtttga caaactggcg aaagacaaga acttcgacaa catccagaag gcgatgtata
    13861 gccagttcga aaacaccttt atgatgtatt tgccgcgcct gtgcgaacac tgcctgaacc
    13921 cggcatgtgt ggcgacctgc ccgagcggtg cgatttacaa gcgtgaagaa gatggcatcg
    13981 tcctgatcga ccaggataaa tgccgtggct ggcgtatgtg catcaccgga tgcccgtaca
    14041 aaaaaatcta cttcaactgg aagagcggta agtctgagaa gtgcatcttc tgctatccgc
    14101 gtattgaagc gggtcagccg accgtgtgct cagaaacctg tgtcggtcgt atccgttatc
    14161 ttggcgtgct gctgtacgat gccgacgcga ttgaacgtgc agccagcacc gagaacgaga
    14221 aagatcttta ccagcgtcag ctggaggtgt tcctcgatcc gaacgatccg aaagtcatcg
    14281 agcaggcgat taaagacggt attccgctga gcgttattga agccgcacag cagtcgccgg
    14341 tttataaaat ggcaatggaa tggaaactgg cgctgccgct gcatccagaa tatcgcacac
    14401 tgccgatggt ctggtacgtg ccgcctctgt ctccgattca gtctgcagca gacgcgggtg
    14461 agctgggtag caacggcatt ctgccagacg tcgaaagctt gcgtattccg gtacagtatc
    14521 tggcgaatct gctgaccgcc ggtgatacca aaccggtact gcgcgcactg aaacgtatgc
    14581 tggcgatgcg tcattacaaa cgtgctgaaa ccgttgacgg taaagttgat acccgtgcgc
    14641 tggaagaggt cggtctgacc gaagcccagg cacaggagat gtaccgttat ctggctattg
    14701 ctaactacga agatcgcttt gtggtgccga gtagtcatcg tgaactggca cgggaagcct
    14761 tcccggagaa aaatggctgc ggctttacct ttggtgatgg ctgccacggc tcagatacca
    14821 aattcaatct gttcaacagc cgtcgtatcg atgccatcga tgtgaccagc aaaacggagc
    14881 cgcatccatg atcgaactcg tgattgtatc gcgtctcctt gaatatccgg atgctgcctt
    14941 atggcagcat caacaagaga tgtttgaggt gattgctgcg tcgaaaaatc tgtcgaaaga
    15001 agatgcccat gcgctgggca ttttcctgcg cgatttaacg gcgatggatc cgctcgatgc
    15061 ccaggcccag tacagcgaac tgttcgaccg tggccgcgcc acgtcactgt tgctatttga
    15121 acatgttcac ggtgaatccc gtgaccgcgg ccaggcgatg gtggacctgc tggcgcagta
    15181 cgagcagcac ggcttgcagt taaacagccg cgaattaccg gaccatctgc cgctgtatct
    15241 ggagtacctg tcgcagctgc cgcaaagcga agccgtggaa ggtttgaaag atatcgcgcc
    15301 gattctggca ttgctgagcg cgcgtctgca acagcgtgaa agccgttatg ccgtgatgtt
    15361 tgatctgctg ctgaaattgg ccaataccgc tatcgacagc gacaaagtgg cggaaaaaat
    15421 tgccgacgaa gcgcgcgatg atacgccgca ggcgctggat gctgtctggg aagaagagca
    15481 ggttaaattc tttgctgaca aaggctgcgg tgattcagca atcactgctc atcagcgtcg
    15541 ctttgccggt gccgtcgcgc cgcaatatct gaatatcacc accggaggac agcactaatg
    15601 caattcctga atatgttctt ctttgatatc tacccgtaca tagccggggc ggtcttcctg
    15661 attggtagct ggctgcgtta tgactacggg cagtacacct ggcgcgcggc gtccagccag
    15721 atgctggatc gcaaagggat gaacctggcg tcgaacctgt tccatatcgg tattctgggg
    15781 atttttgctg gtcacttctt cggtatgctg acgccgcact ggatgtatga agcctggctg
    15841 ccgattgaag tgaaacagaa aatggcgatg tttgctggtg gtgccagcgg cgtgctgtgt
    15901 ctgattggcg gcgtgctgtt gctgaagcgt cgtctgttca gcccacgcgt gcgtgcaacc
    15961 actaccggag cggatatcct gatcctgtcg ctgctcgtta tccagtgcgc gctgggcctg
    16021 ttgaccattc cgttctccgc tcagcatatg gacggtagcg agatgatgaa actggttggc
    16081 tgggcgcagt cggtggtgac cttccacggt ggcgcttctc aacacctcga tggtgtggcg
    16141 tttatcttcc gtctgcacct ggtgctgggg atgacgttat tcctgctgtt cccgttctcg
    16201 cgtctggtac acatctggag cgtaccggtg gagtatctga cacgtaagta ccagctggtg
    16261 cgcgctcgtc actaaacgaa ttttagttca catagaccct gcttcggcgg ggttttttat
    16321 gggcacggtg cggggtgagt tgtcggatgc gcttcgctta tccgacctac aggggaggat
    16381 attgtaggcc cggtaaacgt ggtgccatcg ggcaaaatta gctcggagta acaggttttg
    16441 attattcaag gaagcgatgg tggtggggga aggattactc agcgctgcgc gcttcgccct
    16501 tcgggtcgtt gcctgcggca acgctctctc gctggcgctc aagtcgaacc ttggtcgaag
    16561 cttctcatcc ttccccgctt gggcagaata tttaattgcg gattcgtttg agagtttacg
    16621 gacttttgaa aagtgatggt ggtgggggaa ggattcgaac cttcgaagt
//
LOCUS       DAZPEB010000080        16619 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZPEB010000080 DAZPEB010000000
VERSION     DAZPEB010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16619
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.081"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..244)
                     /locus_tag="ACHURJ_002246"
     CDS             complement(<1..244)
                     /locus_tag="ACHURJ_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR1281184.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPIVTWI"
     gene            351..647
                     /locus_tag="ACHURJ_002247"
     CDS             351..647
                     /locus_tag="ACHURJ_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFR1281185.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(781..1206)
                     /gene="arsC"
                     /locus_tag="ACHURJ_002248"
     CDS             complement(781..1206)
                     /gene="arsC"
                     /locus_tag="ACHURJ_002248"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFR1281186.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(1219..2508)
                     /gene="arsB"
                     /locus_tag="ACHURJ_002249"
     CDS             complement(1219..2508)
                     /gene="arsB"
                     /locus_tag="ACHURJ_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFR1281187.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHILSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(2563..2916)
                     /gene="arsR"
                     /locus_tag="ACHURJ_002250"
     CDS             complement(2563..2916)
                     /gene="arsR"
                     /locus_tag="ACHURJ_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFR1281188.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRN
                     LARQNCSADSKNICS"
     gene            3655..3738
                     /gene="dinQ"
                     /locus_tag="ACHURJ_002251"
     CDS             3655..3738
                     /gene="dinQ"
                     /locus_tag="ACHURJ_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFR1281189.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(3792..5144)
                     /gene="gorA"
                     /locus_tag="ACHURJ_002252"
     CDS             complement(3792..5144)
                     /gene="gorA"
                     /locus_tag="ACHURJ_002252"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFR1281190.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKMLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSEHLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGSEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(5216..6058)
                     /gene="rlmJ"
                     /locus_tag="ACHURJ_002253"
     CDS             complement(5216..6058)
                     /gene="rlmJ"
                     /locus_tag="ACHURJ_002253"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFR1281191.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            6261..8303
                     /gene="prlC"
                     /locus_tag="ACHURJ_002254"
     CDS             6261..8303
                     /gene="prlC"
                     /locus_tag="ACHURJ_002254"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFR1281192.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTQALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            8311..9063
                     /gene="rsmJ"
                     /locus_tag="ACHURJ_002255"
     CDS             8311..9063
                     /gene="rsmJ"
                     /locus_tag="ACHURJ_002255"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFR1281193.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVTTPNAVVTKGHRFDIYAGTPV"
     gene            complement(9112..10581)
                     /gene="dtpB"
                     /locus_tag="ACHURJ_002256"
     CDS             complement(9112..10581)
                     /gene="dtpB"
                     /locus_tag="ACHURJ_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFR1281194.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINSVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            10747..10800
                     /locus_tag="ACHURJ_002257"
     CDS             10747..10800
                     /locus_tag="ACHURJ_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1281195.1"
                     /translation="MVLNVVLAGDFDCVRRS"
     gene            complement(10899..11333)
                     /gene="uspA"
                     /locus_tag="ACHURJ_002258"
     CDS             complement(10899..11333)
                     /gene="uspA"
                     /locus_tag="ACHURJ_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFR1281196.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            11724..12059
                     /gene="uspB"
                     /locus_tag="ACHURJ_002259"
     CDS             11724..12059
                     /gene="uspB"
                     /locus_tag="ACHURJ_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFR1281197.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(12303..13802)
                     /gene="pitA"
                     /locus_tag="ACHURJ_002260"
     CDS             complement(12303..13802)
                     /gene="pitA"
                     /locus_tag="ACHURJ_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFR1281198.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKIPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            14034..15236
                     /locus_tag="ACHURJ_002261"
     CDS             14034..15236
                     /locus_tag="ACHURJ_002261"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFR1281199.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            complement(15551..16603)
                     /locus_tag="ACHURJ_002262"
     CDS             complement(15551..16603)
                     /locus_tag="ACHURJ_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HFR1281200.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNILFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGYVIFGIGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILIYLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
BASE COUNT         4268 a         4236 c         4314 g         3801 t
ORIGIN      
        1 caatccacgt aacaatgggt attgtcgagc aactacagaa cggggtaacg gctcccaaca
       61 gagcagccag gagatagcct ctcccttttc tggcccccat catctgctgg attttatggt
      121 ctggcacctt ttgtcttatc aggctgacac cggcactaat cactataaac agcaacgaaa
      181 gctcgacggc gagaaacaca aacatctctg cggcatcttg cagcatcgca agccaactac
      241 tcatacgtaa cctcattgga taaatctata tttccagaat aatcgaaatg aaattcagat
      301 caagatattt ctggtattat cgaaatatca accagacaga ggggtgtcat atgcagttgg
      361 aagaagtcgc taaagcgctg aaagagctag gtcacccgac ccgtttgttc atatttaaac
      421 atcttgtcaa agccggggaa caagggttgc cggtcggtga gttgcaaaag cagcttggga
      481 tacctggctc cacactcagc caccatatat cagcactggt atcggtagga ctggtcaaac
      541 aaaaccgtga gagtcggacg ctgatgtgtg tttcgcaata cgcaatgctg gaagcgataa
      601 ttgaattctt acgcgaggag tgctgcgtaa atagcaaaat tgcctgatgc gctacgctct
      661 acatatttca tgccatatat tgaatttgca cggttttgta ggtcggataa gacgcgttag
      721 cgtcgcatcc gacataaaca aagcgcactt taccaactat cggcagtgga tatcgccgct
      781 ttatttcagg cgcttacccg cttcatcaac cactttctcg ccatcttcct tggagaatgc
      841 gcctttttgc gcatctggca gaatttccag caccacttct gaagggcggc acaggcgagt
      901 tcccagcggc gtcaccacaa tcgggcgatt aatcagaatc gggtgctgaa gcataaagtc
      961 gattaaccga tcgtcagtaa atttatcttc cgcaaggccc agctcctcat acggttcgac
     1021 gtttttacgc agcagcgcgc gtacggaaat ccccatatcg gcaatgagtt tgaccagttc
     1081 atcgcgcgtt ggcggagttt ccagataatg gataatggtc ggttctgtgc cgctgttgcg
     1141 gatcatctcc agcgtattac gcgacgtgcc gcaggccggg ttgtgataaa tggtaatgtt
     1201 gctcatatca gtatctcatt acaaagtgaa agagagacgt agcgccagcg cagccagcgt
     1261 cacaaacagc acaggcaggg tcatgataat ccctgtacgg aagtaatatc cccagctgat
     1321 agtcatattc ttctgcgaaa gtatgtgcag ccagagtagt gtagccaggc taccaattgg
     1381 ggtaattttc ggtcccaaat cgcagccaat cacattggca taaaccatcg cttctttgat
     1441 aacgccagat gccgtgctgc catcaatgga caacgcgcca accagtaccg tcggcatatt
     1501 gttcataata gaagagagga aggcggtgag gaatccggtg ccgagcgtcg cggcccacag
     1561 gccgttatcc gccagcacgt tgagtacgcc agaaagatat tccgttaatc cggcattgcg
     1621 caggccataa accaccagat acatgccgag cgagaagatg acaatttgcc agggggcacc
     1681 gcgcaggact ttacccgtat taatcgcatg accgcgttta gcgacgacaa ataatatcag
     1741 cgcgcccaca gctgcaatgg cgctcaccgg aatgccgagc ggttccagga cgaaaaatcc
     1801 caccagcaga agcagtaaaa caacccagcc agttttgaac gtagcaggat ctttgatcgc
     1861 ttctgcggga gatttcagca gcgccatatc gtagttctgc ggaatatctt tgcgaaaata
     1921 gagatgtagc atcaccagcg tggcaacaat cgcggcgata tccaccggca ccatcaccga
     1981 ggcgtattcg cgaaagccga ggccaaagaa atcagcggaa acgatattca ccaggttgga
     2041 gacaataagc ggcaggctgg cggtatcggc aatgaatccg gccgccatca cgaacgccag
     2101 cgtcgtgcct ttactgaatc ctaaagccag cagcatggcg atgacaatcg gcgtcaaaat
     2161 aagcgctgcg ccatcattgg caaacagggc ggcaacggca gcaccgagca ggacaatcca
     2221 ggtaaacagc aagcgaccac gaccattacc ccagcgtgag acgtgcagcg ccgcccattc
     2281 aaaaaagccg gactcatcca gcagcaggct gatgataatg acggcgataa acgcagccgt
     2341 cgcgttccag acgatattcc acaccaccgg aatatcaccc ggatggacca cgcccgtaac
     2401 taacgccagt actgcgccga gcgttgcact ccagccgatg cctaaacctt tcggctgcca
     2461 gataaccaat acaatggtca ggacaaagat agcgcctgcc agtaacataa tgcctcctgg
     2521 ataaaacaca tgcgaaaata catatgtgtt tagctaaatt ttttaactgc aaatgttctt
     2581 actgtctgcg gaacagtttt gtcgagccag gttgcggaca attgcctgaa tcttttcctg
     2641 ttcacatcgc caggcttcat caattatttt cgccgcccat gctggaatat gcggcgataa
     2701 gcggtaatga acccatttac cttgcttgcg gtccagcaat agcccgcttt cacgcagcaa
     2761 tgccaggtgg cgggagatct tgggctgcga ctggtcgaga gcagtgcaga gatcgcagac
     2821 gcataactct cccagttcgc tgagcagtaa aacgatgccc agacgggttt catcagcaag
     2881 aattttgaac aattggatgg gtaacagaaa tgacatattg cgctcctgat tgttgcaggt
     2941 agtgtctctc ttcgaagcgg ataagtcaaa aacatatatg atttaacgaa tgtgtaagtg
     3001 cagaggaagg taataggtgt gaattttgag ttggctattc atttgaaagg aggtctgaat
     3061 caggacgtaa aaaaaccgac tttgcgtcgg ttttttactt tccagccctg agttggtggc
     3121 tctggctgga gtgagagagt tcttatctaa cagctcaata ctattagcca gtggctaaca
     3181 ttgcaagaat tagcgatttc ttcagctggc ggagggagca cttcggtata tatcgcagtg
     3241 acgatttata tactttcact gggtcatcgt catattaagc cttgtccgaa tccggatgta
     3301 aaaaaccgac tttgcgtcgg ttttttactt tccagccctg agttggtggc tctgactgga
     3361 gtgagatagc catcatctaa cgcatcaatg ctaataccat actgaaacta ttgcaaggac
     3421 gtgctggttt tataacctgc atgtactgta tgattatcca gttagctctg aggcattttc
     3481 actctggcaa tgcgcataaa cgctttcaaa gtcctggtca gaagtacggg tggtgccgtt
     3541 aactgatgct ctggccggag tgagagagtt cttatctaac aatgagacat gcgccgtgac
     3601 aggcagtgga tgagtaagcg gatgcattct cactccatcg catggagaaa acgggtgatt
     3661 gataaagcaa tcatcgttct aggggcgtta attgcgctgc tggaactgat ccgctttctg
     3721 cttcagcttc tgaactgata gcggaaacgt aattaagggc taagagcaca ctactcttag
     3781 ccctttaaca tttaacgcat tgtcacgaac tcttctgccg ccgttgggtg aatggcgacg
     3841 gtattgtcga agtctttttt ggttgccccc atcttcagcg ccaccgcgaa gccctgcaac
     3901 atttcgtcca taccaaagcc aatgccgtga ataccgacaa tcttctcttc cgatccaacg
     3961 cacaccagct tcatgcggca cggctggcgg tgagtggtga cggcggtata catcgcggtg
     4021 aaagaggatt tatacacttt cacctgatcg tcgccatact gctcgcgcgc ctgcggttcc
     4081 gttaaaccaa cagtaccaat cggcggatgg ctgaagacca cggtcggaat gttgctgtaa
     4141 tccagatgct catccggctt gttattaaac aggtgttcag agagacggcg acccgctgca
     4201 actgccaccg gtgtcagctc cactgcaccc gtgttatcgc ccaccgcgta aataccttca
     4261 atgttggtgt tttgatattt atcgacgacg atatagcctt tttcgttggt tttaacgcca
     4321 gcggcttcga ggttgatgtt gtcattggca ggctcgcgac caatcgccca aatcaggcaa
     4381 tccaccgttt cactgcgacc atcttccagc tccagcgtca ggctaccatc ggcatttttc
     4441 actaccgctt tcgggatggc gttggtgtgc agctgcgggc cttcggcgtt catcacttcg
     4501 accagcgttt cggaaatcat cgggtcgaag ctgcgcagcg gcgcatgttt acgcacaaac
     4561 agatgcgttt tcgcgccgag gccgttaatc acgcccgcca gctcaacggc gatgtaaccc
     4621 gcgccaacaa ccgccacgcg ctctggcaaa gcaggaaggg cgaagaagcc atcagaatca
     4681 ataccgtatt ccacgcccgg aatatccggg tggctcggac gaccgcctgt ggcgatcaga
     4741 atatgatcgg ccgtgatggt ttcgccgttt acctccagca ttttggcatc aacgaagcgg
     4801 gcaaagcctt tgattacatc aacgttattt ttaccgagca cgttttcata ggaagtatga
     4861 atacggtcga tataggcggt acggctggcg atcaacgttt cccagttgaa cttgttgatg
     4921 gtggtatcga aaccataatc agggccgtac atatggatcg cttcacggat ttgcgccgcg
     4981 tgccacatca cttttttcgg cacgcagccg acgttcacgc aggtgccgcc cagctctttg
     5041 gcttcaatca gtgcgcattt ctggccgtac atagccgcgc ggttgatgga ggctataccg
     5101 ccgctgccgc cgccgatggc gatgtaatca tagtgtttag tcatgacaga gtgtccttat
     5161 cgttgattac cgcgattgta gcgcgagacg taataggtgc cagcaatggc tgcaattact
     5221 ccggcacgat ccagcttacg gtggcgtgcc cggtgcctgc cggaaccagt ttgctgtgca
     5281 gccacggcag cacgttattc atctgctgtt ccagtttcca cggcgggtta atcacaatca
     5341 tgccggaagc ggtcatgcca cggcgatcgc tgtctggcag taccgccagt tcaatttgca
     5401 gaattttgcg aataccggtc gcttccagat cgtggatcat gcgcttaatt tgctgacgca
     5461 gcaccaccgg ataccacagt gcgtaagtac cagtggcgaa acgtttgtaa ccttctgcta
     5521 tcccgctgac caccgcctga tagtcggttt tcatttcata cggcgggtcg ataaggatta
     5581 aaccacggcg ggaaaccggc ggcagtttgg ccttaagctg ctggaaacca tcggcttttt
     5641 cgacgcgcgc acggctgtct ttctgaaatt cagaacgcag caacgggtaa tcgctcgggt
     5701 gcagttcggt cagttgcagg ctgtcttgtt cgcgcagtag ctggcgagca atcaatggcg
     5761 aaccggggta gtaacgcaac tgaccgctac ggttgaagtg ttttaccaca ttgatgtacg
     5821 cctccagttc tgcgggcaaa tcgtcctgct gccagatacg ggcgatgcct tcgagatatt
     5881 cgccggtacg ctcggcatgt tcgctgccta actgataacg cccggcccct gcgtgggtgt
     5941 cgagatagag aaacggttta tctttctctt tcagcgactc gatgatcagg ctctgaacgg
     6001 tatgtttaag gacgtcggcg tggttgccag cgtgaaagct gtggcgataa ctgagcatgg
     6061 gtaaaggtgt tccggtaagt aaaaatcggc ccgtattcgg ggcgcacaaa agcgtatcag
     6121 gacagtatac cgaaaagagg ccgccgccgc gaaagcgtaa cgtttctcat tgaaattcac
     6181 tacacttaac cccatgctac acacattatg taaagcgcct gttgagcgct tccttaacct
     6241 ctttaaccag gactgcgcga atgacgaatc cgttactgac tccctttgaa ttgcctccgt
     6301 tttctaaaat tctcccggaa catgtcgttc cagccgtgac tcaggcgctg aacgattgcc
     6361 gcgaaaatgt ggagcgcgta gtagcgcaag gggcaccgta cacctgggaa aatctctgcc
     6421 agccgttggc ggaagtggac gatgtgttgg ggcgtatctt ctccccggtc agccacctga
     6481 actcggtgaa aaatagcccg gaactgcgtg aagcctacga gcaaaccctg ccgctgctgt
     6541 cggaatacag cacctgggta gggcaacatg aagggctgta taaagcgtac cgcgacctgc
     6601 gcgatggcga tcattacgcc acgctgaaca cggcgcagaa gaaagcggtt gataacgccc
     6661 tgcgtgattt tgaactctct ggcataggtc tgccgaaaga gaaacagcag cgttacggcg
     6721 aaattgcgac ccgtctttct gaactgggca accagtacag caacaacgtc ctcgatgcga
     6781 caatgggctg gaccaaactc gttaccgacg aagcggagct ggcggggatg cctgaaagcg
     6841 cgctggctgc ggcaaaagcc caggccgaag cgaaagagct ggaaggctac ctgctgacgc
     6901 tggatatccc aagctacctg ccggtaatga cctactgcga caaccaggcc ttgcgtgaag
     6961 agatgtatcg cgcttacagc acccgcgcct ccgatcaagg cccgaacgcc ggtaaatggg
     7021 ataacagcaa ggtgatggaa gagatcctcg ctctgcgtca cgaactggcg caactgctgg
     7081 gctttgaaaa ctacgccttt aaatcccttg ccactaaaat ggcagaaaac ccgcagcagg
     7141 tgctggattt cttaaccgat ctggcaaaac gcgcgcgtcc gcaaggcgaa aaagagctgg
     7201 cacaactgcg cgccttcgcc aaagcggaat ttggcgtcga tgagttgcag ccgtgggata
     7261 tcgcgtacta cagcgaaaaa caaaaacagc acctctacag catcagtgac gaacagctgc
     7321 gtccgtactt cccggaaaac aaagcggtta acggcctgtt tgaagtggtg aaacgtattt
     7381 acggcatcac cgctaaagag cgtaaagatg ttgatgtctg gcatccggat gtacgtttct
     7441 tcgaactgta tgacgagaac aacgaactgc gcggcagctt ctacctcgat ctgtatgccc
     7501 gtgaaaacaa gcgcggcggg gcgtggatgg atgactgcgt aggccagatg cgtaaagctg
     7561 acggttctct gcaaaaaccg gtcgcgtatt tgacttgtaa cttcaaccgc ccggtaaatg
     7621 gtaaaccggc gctgttcact cacgacgaag tgatcaccct gttccacgag ttcggtcacg
     7681 gtctgcacca tatgctgacc cgcatcgaaa ccgctggtgt ttccggtatc agcggtgtgc
     7741 cgtgggatgc ggtcgaactg ccgagtcagt ttatggaaaa ctggtgctgg gagccggagg
     7801 cgctggcgtt tatctctggt cactatgaaa ccggcgaacc gctgccgaaa gagttgctgg
     7861 ataaaatgct ggcggcgaag aactaccagg cggcgctgtt tattctgcgc cagctggagt
     7921 tcggcctgtt cgatttccgc ctccatgccg agttccgccc ggatcagggt gcgaaaatcc
     7981 tcgaaactct ggcagaaatc aagaaactgg ttgccgtagt accgtctcca tcctggggcc
     8041 gtttcccgca cgctttcagc catattttcg ccggtggtta tgccgcaggt tactacagct
     8101 acctgtgggc tgacgtactg gcggcagatg ccttctcgcg ctttgaggaa gagggcattt
     8161 tcaaccgtga aaccgggcag tcgttcctcg acaacattct gagccgtggc ggttcagaag
     8221 agccgatgga tctgttcaaa cgcttccgtg gtcgtgaacc gcagctggat gcgatgctgg
     8281 agcattacgg cattaagggc tgatcattca gtgaaaatct gcttaattga tgaaacaggc
     8341 gccggagacg gcgccttatc tgttctggcg gcccgctggg ggctggagca cgatgaagac
     8401 aacctgatgg cgctggtgtt aacgccggaa catctggaat tgcgcaagcg tgatgagcca
     8461 aaacttggcg gcatctttgt tgattttgtt ggcggagcga tggcgcaccg acgcaaattc
     8521 ggcggtggtc gcggtgaggc ggtggcgaaa gcggtgggca ttaaaggcga ttatttgccg
     8581 gatgtggtgg acgccaccgc agggctgggg cgcgatgcct ttgtactggc ttcagtcggc
     8641 tgccgcgtgc ggatgctgga gcgtaatcca gtggttgccg cgctactcga cgacggcctg
     8701 gcgcgtggtt atgcggatgc ggaaatcggc ggctggttgc aggagcggtt gcagttaatt
     8761 cacgcctcca gcctgacggc gctaaccgat attaccccac gcccgcaggt ggtctatctc
     8821 gatccgatgt tcccgcataa gcagaaaagc gcgctagtga aaaaagagat gcgtgtcttt
     8881 cagtcgctgg tgggaccgga tcttgatgcc gatggattac tggagcctgc acgcttgctg
     8941 gcgaccaaac gcgtggtggt aaagcgcccg gactacgcgc caccgctagc gaatgtcacc
     9001 acgccaaacg cggttgtgac taaagggcat cgctttgata tctatgcagg tacgcccgtg
     9061 taaaagtaaa gcagggtagc gataacgcta ccctgctttc tgcaagaata attaatggct
     9121 ttccggcgtc gcaatcatgc gtttcagcca cggcaccatc aacagcatca ctactgcaac
     9181 gcccagcgtg accagaccaa tcttaccaaa cacgttggta tagacgggca acgtctcaag
     9241 cggatcggta atgttgtccg gcaccgcggt aaatgttgcc acatagccgc ccagcaagaa
     9301 cgcggcagcc tgcgtcagga accacatccc gagaataaag cccatcaaat gctgcggcac
     9361 cagggcagca atcatcgcca ggccaagggc gctaataaac agttcaccta agctctggaa
     9421 taagtacacc agcacgataa accatggcga tgtcagccct tgtgcatccg caaaccacat
     9481 tcccgcagct gccgccgtca aaaagcccag tgagcacata aacatgccga gagtaaattt
     9541 catcggcatc gagaggtctt tgcctttgtt acccagatgc gtgtaaatgc ctgccagtat
     9601 tgggctggcg agtaccaccc agaacgggtt aagcgcctgg aagctgaccg agttgatgga
     9661 aaaaccgaga atttcatgat gcacgttgtt gatggcaaag aagttcagcg atgttggcat
     9721 ctgggcgtag agaatgtaaa acaccaccgc ttcgagcatc aggacaaagg cgacaaacat
     9781 tttattgcgc ccggttttat ccagcttgaa tgcctgacga aagaagatga tggtgacgac
     9841 gatggagaga acaatcagca ccagattggc gacttctacg ttgtgcatca gccatgcaca
     9901 tacgaagatc atcaccacgc tgccaagtaa tacgtacaac agtttgctga agctcatcgg
     9961 cttgaagtcg ggttcagaac caatgtcttt caccattcca cgacaggcga tgtaaaccag
    10021 taatgcgata attaaccccg ccccgcacag gttgtaggtg actgaataac cgaatctatc
    10081 agcgatcaca ggggccagcg ataacgctat caacgagccg atattgatcg acatatagaa
    10141 cagggtgaat gcgccatcaa gccgcggatc tttcggcgga tagcacttcg aaagcaagct
    10201 ggctgggtta gctttaaaca ggccgttacc gacagcgata gtccccaggg cgatgaaaat
    10261 caggtcaggc ttaagtagcg acatgccggt catgaagtag ccaatcgcca gcacaagtgc
    10321 tccgagaaca atggtgcgtt tggtccccag caggtggtcg ccgacatagc cgccaatgga
    10381 aatgaggcca tagaccagcg cagcaaaagc accaaaagtg acaaaagcct gctcttgcga
    10441 gaatccaagc tgtttaacga agaaaaccgc cagtacgccc tgcacgccgt agtagccgaa
    10501 tcgctcccat aactcgacaa aaaagatcat gaaaaatggg cgaggttgct gcagcatccc
    10561 catgggtgtt gttgtattca tatttattaa ccttccaata ccatcctgaa aagtatgaac
    10621 gcgtaaggga taacgcgcga aaaagtttac atctgtcaga gattcttaat attgatgcgg
    10681 tgaatggatt aatacactat tacaacagaa aataaccagc taatcacact aatgatggaa
    10741 aatatgatgg tgttaaatgt tgtattggca ggagattttg attgcgtacg cagatcgtag
    10801 gccagataag gggtttacgc tgcatcaggt aacaaggcgc gccaaaaagc aaaacgcccg
    10861 catatgcggg cgttcaggaa cacgtcgtgg agggaagatt attcttcttc gtcgcgcagc
    10921 ggaacaatca gcatatcaac gtgaacggtg ttgatcagct gacgtgcgga agacatcagt
    10981 ttgctccaga agtcctggtg gtgaccacaa accaccagat ccatatcgta tttcttgatt
    11041 gcatcgacca gaacctggcc caggtcgccg ctgccgctca gggtttcagt gattgggtag
    11101 cctgcattag tggaaagctc ggtcagcgca tgatgtgtct cttcagagat gcgtttctgc
    11161 atatcaccca gattcacatc aataagcccg gtgtataggt cagagtagtt tacatctacg
    11221 tggatcagag aaactttcgc attgtagggg cgagccatag agactgcttt ctctaccaga
    11281 actttgcttt ccggggagag atcgaccgcg atgagaatgt gtttataagc catagtgtta
    11341 ctccttccat aaagttgtcg atgactggcc agctagcgtt tcttgtgccg ttacgtcacc
    11401 cgcgtcctgc gaactacgtg cgcggggcta acccagccgt aaagattcag tgttaagacc
    11461 atccttacct tatagcgacc cggatgatcc gtcaatccgc cttgcttacc cgttagataa
    11521 acaaattttc gacatgaata tattgatagt ggttaacctt ctggaaaaaa aacaacctga
    11581 tctcctacac tatctataga gccgctcgga tgttggtcac cacaaaagcg gtttctccag
    11641 gacctggtgt taactgattg gcagcgtatc gggagcggta gccccggggt ggaactccgc
    11701 gggcaggtcg ccggggagga ggtatgataa gcaccgtcgc attattttgg gctttatgtg
    11761 tcgtttgcat tgttaacatg gcgcgctatt tctcatcact acgcgcgttg ttagtggtac
    11821 tgcgtaactg cgatccattg ctctatcaat atgttgatgg agggggcttt tttacctcac
    11881 atggccaacc caacaaacag gtgcgtctcg tttggtatat ctatgcccaa cgttatcgcg
    11941 atcatcacga tgatgagttt attcgccgct gtgagcgggt gcgtcggcag tttattctga
    12001 ctagcgcatt gtgtggtctg gtggtggtca gcctgattgc attgatgatt tggcattaag
    12061 cacttcagca ataataacgc ataaaaaaag ctggccagta aactgacccg ctcgagactg
    12121 attacaaacg caaaattgcc tgatgcgcta cgcttatcag gcctgcataa tctctgcaat
    12181 ctattgaatt tgcatgtttt tgtaggccga ataaggcgtt cacgccgcat ccggcatgaa
    12241 caaagcgcac tatgtcacaa tctgaagcgg gccagctgac ccgctcgttt tggtgcgtac
    12301 gattacagga actgcaagga gagccagtac agcccgccgg aaagcagtac cgcagccgga
    12361 agggtaaaca cccaggccat cagaatgctg gtcacggttt tacgctgtaa gccgccacca
    12421 tctaccacca tcgtccccgc gacagaagag gagagtacgt gagtagtgga aaccggcatc
    12481 ccggtataac tcgccaggcc gatagacact gccgccgtca tctgggcaga catcccctga
    12541 gcgtaggtca tgcctttctt accgattttc tcaccgatag tcgttgccac acgacgccag
    12601 ccaatcatcg taccgatacc taacgccagc gcgaccgcca tgatgatcca caccggtgca
    12661 tactcgatgg tgctaagcat gtcggacttc agtttcttca acaggcgctg atcgtcagca
    12721 ctcacgccag gtatcttcac tactttgtcg atagtgtcag aaacgcacag cataatgcgg
    12781 cgcatctggc tacgttgatc aagcgacagc ttgtcgtagc tttccacatc ggtagttaac
    12841 atacctttca ggcggttgag cgcgttaatg gtattcgacg gatggcaatg gaactccgcg
    12901 ggttgcgttg cgccagcttc cggagccggt actaactgat cagcaccggt agcctgtttc
    12961 agcagcgcag gatgctgctc aaagtaagct tcgacgttgt tgatggcatc acgggtacgg
    13021 gtgatttcgt agccagtggc attcatattc accacgaagc ctgctggtgc gacaccaatc
    13081 aataccaaca taaccagacc aatgcctttc tgaccatcgt tcgcgccgtg cgaaaacgcc
    13141 acgccgatag cggaaaggat cagcgcaata cgcgtccaga acggcggctt tttcttgccg
    13201 tctttctttt cacgctccgc tggggtcagg tggatacggg cgcgtttctt ggtgccgctc
    13261 cagtagcgac gcagcaagaa aatcagaccg ccagcaaaca ccaggccgac aataggggaa
    13321 acaatcagag aaccgaaaat acttaatact ttcgggatat tgagtgcatc caccactgac
    13381 gtcccggtca tcaacgcatt ggttaaacca atcccgatga tcgcgccaat cagtgtatga
    13441 gagctggatg caggtaaacc aaagtaccag gtacccaggt tccagataat cgccgccagc
    13501 aacatagaga acaccatggc aaggccatga gacgatccca tattaagcag cagatccgtc
    13561 ggcagcatat gcacaatggc ataggcaaca ctcagaccac ccagcaaaac acccaaaaag
    13621 ttgaacaccg ccgccataac cacggcgagc tgagaacgca tcgcgcgggt atagataacg
    13681 gttgccacgg cgttggctgt gtcatggaaa ccattgatgg cttcgtagaa cagcacaaaa
    13741 gccagtgcaa gcaataataa cagcccggta tgcaaatcca ggccagcaaa caaatgtagc
    13801 ataggacgtt acgccatttt gaggacatga acgcggcgca ttatcagtga ctttcgcggc
    13861 gcgggcaaag tgaaatatag actttttttg atttgcctcc tgtatggatt tcactcaaaa
    13921 aataattatc ttatataatt caggcaaata cttcctttta gtaatattga tgctggtgtg
    13981 accactgagg aatctttaca attcacgccc gttttttcta agaggagcgc aacgtggaaa
    14041 ggtttgatgc cattattata ggcgctggtg cggcgggtat gttctgttct gcgctggcag
    14101 gtcaggcagg acgccgggtt ctgctgatcg ataatggtaa aaaaccaggg cgcaaaatcc
    14161 ttatgtctgg cggtgggcgc tgcaacttta ccaaccttta tgtcgaacca ggcgcttatc
    14221 tgagccagaa tccgcatttt tgtaagtctg cactcgcgcg ttttacccag tgggatttca
    14281 ttgatctggt caataaacac ggcatcgcct ggcacgagaa aacgttagga caactcttct
    14341 gcgatgactc cgcgcagcag attgtcgaca tgctggtgga tgagtgcgag aagggcaatg
    14401 taaccttcag attgcgtagc gaagtgctga gtgtggcgaa ggatgaaaca ggcttcacgc
    14461 ttgaactgaa cggcatgact gtcggttgcg aaaagctggt catcgcgacc ggtgggctgt
    14521 caatgccggg gctgggcgca tcgccgtttg gttataagat tgccgaacaa tttggcctca
    14581 acgtgctgcc gacccgtgcg ggtctggtgc cattcactct gcataaaccg ttgctcgaag
    14641 agttacaggt gctggcgggc gtggcggtgc cttccgtgat taccgctgaa aacggcaccg
    14701 ttttccgtga gaacttactc ttcacccacc gcggcttatc tggaccggcg gtgttgcaga
    14761 tttccagcta ctggcaaccg ggtgaatttg tcagtatcaa tctgctaccg gatgtggacc
    14821 tcgaaacctt cctgaatgag cagcgtaacg cacatccgaa tcagagcctg aaaaacacac
    14881 tggcggttca tctaccgaag cggttggttg aacgcttaca gcaactcggg caaatcccgg
    14941 atgtttcgct aaaacagctc aacgtgcgtg accaacaggc actgattagc acattgaccg
    15001 actggcgcgt acaacccaac ggcactgaag gctatcgcac tgccgaagtg acgctcggcg
    15061 gcgtggacac caacgaactc tcttcacgga cgatggaagc gcgcaaagtg cctgggctgt
    15121 acttcatcgg cgaagtgatg gacgttaccg gctggctggg gggctataac ttccagtggg
    15181 cgtggtcgag tgcgtgggct tgtgcgcagg atttgattgc agcgaagtcg tcctgacctg
    15241 ttacctaaag atgaaaatta ttagagccgc attaaaaatg agctaatttt gatagtggct
    15301 atcttgtgat tattttctaa tgagcccgtg aactgaaacc ctccaggctt aatataaggt
    15361 ggaaggaaag atgattgaaa actcacccag tggccagatc tttataaaat atgaacaggc
    15421 tgaaaaagac agtaactttg ttgtttttta tccttaacca ctatgcatta atgctgcgtt
    15481 atttcatgat gcctaagaaa aaccagcgtt acgcaaatgg tcaacgctgg ttttatccgg
    15541 tacgttgcaa ttatttttta gcagaacctg cttctaatat tgaaacgatt gagaacaacg
    15601 taaagcatac cgctcccaaa ccgaccaaaa cccgcgaagg aataaaatat cccatgtcgg
    15661 tctgcgccat ttccgcaaga aacgatgcca ggaaaaggca aaacaggcag gtgaagacgg
    15721 gaatcatcgg tattctgttg gctaacgaac aggtacgtct ccataccagt gccagtagcc
    15781 agacttttga gaatatgctg taacagatca ttccgaggta aataagaata atgccgggag
    15841 ccagtcgggc gcttgcatcg gaactgacta agacgtatat cccctgcagt accgtgattg
    15901 agccgagaaa aataacccaa taacaccaca gccagtgttc tttagttgaa aacgtattac
    15961 gtgtttgatg gacaattgtg gcaacaaggc caattagaca ggcacagatt gcggttaacc
    16021 caagcaatac gtgacccgcg acataatgcg gagttatgtc agcactacgt aacagcgtaa
    16081 tagaccaaat gaaaccgagc agggtgagta aaacgggaac ggcaatgagg cagttaccga
    16141 ttaatgaaga ataagcctgt accggtgttc cgtcgctctt gctccccgct gcatttttgg
    16201 gaatgagcag aaagtgaccg gacgacgctg ccaccgtcga tacacatgcg gcaatcatac
    16261 caatgccgaa aataacatag ccggcgacaa actcgtctgc catcacatca ttgcctgcta
    16321 acagtgccca accccatatc atggtgataa ttgaccctgc atagccaata atggggaaca
    16381 atatattgta aaacgtatta acgccgcgcg tgagctgcga aatgataata aatgcggtag
    16441 tgaataatgc caggcatatg gctgccagag aaatcagcac atgacccgcg acgaaatact
    16501 cgctttgccc tgaactttcc agcacaaagc cacctaacgc gatacaaatt aagcccataa
    16561 tcaaagggat taatttgaaa agccacccga tatatatgtt cactttaaac tcctcaggt
//
LOCUS       DAZPEB010000081        16606 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZPEB010000081 DAZPEB010000000
VERSION     DAZPEB010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16606
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.082"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(28..2217)
                     /locus_tag="ACHURJ_002263"
     CDS             complement(28..2217)
                     /locus_tag="ACHURJ_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFR1281201.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSICRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(2426..2501)
                     /locus_tag="ACHURJ_002264"
     tRNA            complement(2426..2501)
                     /locus_tag="ACHURJ_002264"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2466..2468),aa:Ala,seq:ggc)
     gene            complement(2541..2616)
                     /locus_tag="ACHURJ_002265"
     tRNA            complement(2541..2616)
                     /locus_tag="ACHURJ_002265"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2581..2583),aa:Ala,seq:ggc)
     gene            2837..3196
                     /locus_tag="ACHURJ_002266"
     CDS             2837..3196
                     /locus_tag="ACHURJ_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFR1281202.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            3198..3590
                     /locus_tag="ACHURJ_002267"
     CDS             3198..3590
                     /locus_tag="ACHURJ_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFR1281203.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGVRAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(3641..5056)
                     /gene="gltX"
                     /locus_tag="ACHURJ_002268"
     CDS             complement(3641..5056)
                     /gene="gltX"
                     /locus_tag="ACHURJ_002268"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFR1281204.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGR
                     TRSIERINKALAFIAERENQQ"
     gene            5316..5391
                     /locus_tag="ACHURJ_002269"
     tRNA            5316..5391
                     /locus_tag="ACHURJ_002269"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5349..5351,aa:Val,seq:tac)
     gene            5436..5511
                     /locus_tag="ACHURJ_002270"
     tRNA            5436..5511
                     /locus_tag="ACHURJ_002270"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5469..5471,aa:Val,seq:tac)
     gene            5558..5633
                     /locus_tag="ACHURJ_002271"
     tRNA            5558..5633
                     /locus_tag="ACHURJ_002271"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5591..5593,aa:Val,seq:tac)
     gene            5638..5713
                     /locus_tag="ACHURJ_002272"
     tRNA            5638..5713
                     /locus_tag="ACHURJ_002272"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5671..5673,aa:Lys,seq:ttt)
     gene            5833..6174
                     /locus_tag="ACHURJ_002273"
     CDS             5833..6174
                     /locus_tag="ACHURJ_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HFR1281205.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLDVSLLNP
                     VEKTTHIDIYI"
     gene            complement(6165..7091)
                     /locus_tag="ACHURJ_002274"
     CDS             complement(6165..7091)
                     /locus_tag="ACHURJ_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1281206.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            7181..8179
                     /locus_tag="ACHURJ_002275"
     CDS             7181..8179
                     /locus_tag="ACHURJ_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFR1281207.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCTLPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(8176..8394)
                     /locus_tag="ACHURJ_002276"
     CDS             complement(8176..8394)
                     /locus_tag="ACHURJ_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFR1281208.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(8396..10411)
                     /gene="ligA"
                     /locus_tag="ACHURJ_002277"
     CDS             complement(8396..10411)
                     /gene="ligA"
                     /locus_tag="ACHURJ_002277"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFR1281209.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(10482..11468)
                     /gene="zipA"
                     /locus_tag="ACHURJ_002278"
     CDS             complement(10482..11468)
                     /gene="zipA"
                     /locus_tag="ACHURJ_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFR1281210.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPVYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            11698..12459
                     /gene="cysZ"
                     /locus_tag="ACHURJ_002279"
     CDS             11698..12459
                     /gene="cysZ"
                     /locus_tag="ACHURJ_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFR1281211.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            12644..13615
                     /gene="cysK"
                     /locus_tag="ACHURJ_002280"
     CDS             12644..13615
                     /gene="cysK"
                     /locus_tag="ACHURJ_002280"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFR1281212.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            13999..14256
                     /gene="ptsH"
                     /locus_tag="ACHURJ_002281"
     CDS             13999..14256
                     /gene="ptsH"
                     /locus_tag="ACHURJ_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFR1281213.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            14301..16028
                     /gene="ptsI"
                     /locus_tag="ACHURJ_002282"
     CDS             14301..16028
                     /gene="ptsI"
                     /locus_tag="ACHURJ_002282"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFR1281214.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            16069..16578
                     /gene="crr"
                     /locus_tag="ACHURJ_002283"
     CDS             16069..16578
                     /gene="crr"
                     /locus_tag="ACHURJ_002283"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFR1281215.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLPGSVTVG
                     ETPVIRIKK"
BASE COUNT         4196 a         4273 c         4074 g         4063 t
ORIGIN      
        1 gataattccc cggttttttt acgcctgtta atcagctaat ggctgcgggc gaccaatcaa
       61 atacccttgc agatattgca ccccgagctt atgcaatagc gcctgctgct gttgcgtctc
      121 gacaaactcc gcgaccacac tcaatgactt cgctttcgcc agatcggtaa ttgatctcac
      181 aatcatcgca tccagcgtgt tcgtgacaat atctttcaca aagacgccat caattttgat
      241 gatatcagcc tgcaaacgct ttaaccgttc gtagttggca tagccggtgc caaagtcatc
      301 aatcgcaatc cggaaaccaa acttatgcag ctgctcgatg ttgtacatgc tgctttctgc
      361 gttagaaaac gcctgctcct cggtgatctc aagaatgacc gcctgcgggg agatgtgata
      421 acgcttaaac agacgaataa tccgcccggc aatattcttt tgcagcagcg tgagcggcat
      481 taaattgact gaaaagcgcg gtcctttttt gtcgcaaggg tgtgttgcca gccacttcaa
      541 caaggattcc agcacttgca aatcaaaacg cgcgctaagg ttaaactgag caataagggg
      601 cagaaactta tccggggtca taatgccgcc gtcatatttc agtcgcgcga ggatctcatc
      661 ataaccttca ccctctttgt tgcgaattgg ctgggcgtag agcagcaaat caccctgatc
      721 taacgccgtg cgaatggtat tcagcaatag cacctgttta gtggtctgcc cggaaaccat
      781 ctcctctctg ctatccagcg ccagcacatg atgatgtgcg caggattgct ccgccagcca
      841 gcttaactgc cccaacaagg gttgcagggt ttcctgattt ccatcaaaac gcccccaggc
      901 agcaccatag cccatatcca gcccggtatt gttccagtga atttgccgac tattcaggat
      961 gttaaccata tgctggagtc gcccttccgt ttccggcccg ctcagcacta acagcagttc
     1021 actacccggc aattgataca acttttcgtt ttcctgcatc aacggcagca gcgtacggca
     1081 aattgagcgg atacagtgaa cgcgcatcat taagccgtaa tgacgactca taaactcaag
     1141 attatcaatg cgcaggcagc aaaaactctt gcccgcctct tgctccggcg cttgttccaa
     1201 cgcacggaag ttgggcagga gcgttaacgg atcggtcagc gcctgcaaat gccagcggcg
     1261 attaagccat tcactgcgat gataaatgcg caccatatag agcaggcaaa cgctaaagga
     1321 aatcagcacc gcaagaataa atgccagcga atattcggtt tccacccctt gcaaaaagtt
     1381 ctggttgtaa ttcagaaggc aaagcgttga aacagcccag gtgagattta aaaacggata
     1441 gcgaagctta ccgaccccga gggtaaagat gatgaagaaa acgggtacca ggtaaccggc
     1501 aataaagtcg ttttcataag gtgtgcacaa cagaagtaga agcacgctta acgctgccag
     1561 ccagcttaag gtaaatgcgc gtttctcttt gcccaacgac ggcgcgatat ccctgcgcca
     1621 caatatctgc gcaaagtggg gacttacaat catgcgagtg agatagtaga aaagcatgtt
     1681 gtaaatcagc acagcggtga aaaggcttag caaatcaacg accgtgaaaa tggcatccgc
     1741 atcgccgaaa aaggtggata tctttagcgg gaaatcaaag aagctcccca caagatacat
     1801 gctgcatttg atgccgatcg gcgtcaccaa accaagccag aacagacgtt gccagacata
     1861 tcggctggtc aatccataac gccaacgcgt ccccagctgc cagcgtaata ttgcgcaggc
     1921 acacaacacg acaaacgtct ggctaccgag catgaccgca gtctgtaaaa aagagagatg
     1981 aaagttccac agattggtgc aaaacatccc gcataaaacg ggaacgacac ctcgccagcc
     2041 aaagataaac aagattgaca gcattacgca cagtggcatc cacgccagaa agatataact
     2101 ggaatggata atggcaagtg gcgaaataaa acgggatagc tgaataagta ccacggtgag
     2161 cgtaaacgct agtgtgaata tcttgatatt ttttatcagg ttatgctcca caaacatgag
     2221 tacactactc tcactgatcg gagttttcag gcacacaaat aataagttta ttcgcatcgc
     2281 gaagcaaaca gggggccagc ctgttgccga tttttcatcc aatgatgctt tcaccaggcg
     2341 gcactaaccc ggacgtagac tgtattacaa aagcggcaaa aagcagagac aaaaaacccc
     2401 cgctttgcag cgagggttgg aaatttggtg gagctaagcg ggatcgaacc gctgacctct
     2461 tgcatgccat gcaagcgctc tcccagctga gctatagccc cacgatgcgt ttacgtacca
     2521 aatttgctgg gtgcaaaatt tggtggagct aagcgggatc gaaccgctga cctcttgcat
     2581 gccatgcaag cgctctccca gctgagctat agccccgtca cgtaaagctt gtcgagttga
     2641 cgggcggcat catatgaatt ccgcccgaat gtgtcaacgg caaattgcaa cgtgtagttt
     2701 caatcgctga aaaatcaggc aaatgaacaa ttttggaaaa ccgctcgcat tcagtagtta
     2761 ttcatgtcac ggtttcctgt aaagtggtgt tataaaatga actactaata gacccacata
     2821 cattcaggga attgttatgt tcaaggagag gatgacacca gatgaacttg ccagactgac
     2881 cggctatagc cgccagacca ttaataaatg ggtacgcaag gaaggctgga caacgtcacc
     2941 aaaacctggc gtccagggtg gcaaagcccg actggttcac gtcaatgaac aggttcgtga
     3001 atatattcgc aatgctgaac gtccagaagg tcagggagaa gcgcctgccc tttctggtga
     3061 tgcgccgctt gaagtattgc tggtgacact ggcgaaagag atgacgccag ttgaacaaaa
     3121 acagtttaca tccttgcttc tgcgggaagg gattatcgga ttgttacaac gcttagggat
     3181 tcgcgatagc aaataatatg aaaagattac gcaataaaat gaccaccgaa gaactggctg
     3241 aatgcctcgg tgtggctaaa caaactgtta accgttggat cagagaaaaa ggctggaaaa
     3301 cggaaaaatt tcccggcgtg aaaggcggtc gtgccagact tattctggtc gatacgcaag
     3361 tttgcgagtt tattcagaac acgccagcct tccataacac accaatgttg atggaagctg
     3421 aagagagaat tgctgaatac gctcctggag tacgcgcccc ggcgtatcga caaatcatca
     3481 acgcgataga taatatgaca gacattgagc aggaaaaagt cgcgcaattt ttgtcacgtg
     3541 aaggaattcg caatttcctc gcccgtctcg atattgacga atcagcataa ataaaaaacg
     3601 gcaggatatt atctcctgcc gtttatcttt tatgcgctaa ttactgctgg ttttcgcgtt
     3661 cagcaataaa agccagcgct ttgttgatac gctcaatact gcgggtccta ccaattgcgt
     3721 gaacggtaac atccagtgcc ggagactgcc ccgcaccggt tacggcgaca cgcagcggca
     3781 taccaacttt acccataccc acttccagct catccgccgt cgcctgaata gcgtgatgaa
     3841 cgttttcagc ggtccagtca gtaatcgcgg ccagtttgtc acgaaccact tccagcggct
     3901 gacgcgctac cggacgcaga tgttttttcg cggcgtcggc atcgaactca gcaaaatctt
     3961 cgtagaaata acggcagctc tgtgccatct ctttcagcgt cttgcagcgt tcgcccagca
     4021 gtttcaccag atcagccagc tgcgggccgt tacgggtatc gatattttcc tgctcaatgt
     4081 gccactgtaa gtgagtagca acatactccg gcggcagcgc gttaatgtag tgatggttca
     4141 gccacagcag cttgtcggtg ttgaacgcac tggcagattt gctgacggca ttcagggtga
     4201 agtatttgat catctcttca cgagtgaaga tttcctgatc gccgtgggac cagcccagac
     4261 gcaccagata gttcagcagt gcttctggca aataaccgtc atcacgatac tgcattacgc
     4321 tgaccgcccc gtgacgtttg gacagttttt taccgtcatc gccgttgatc atagaaacgt
     4381 gcgcgtaaac cggcaccggc gctttcaggg ccttaagaat gttgatctgg cgtggcgtgt
     4441 tgttgatatg gtcttcgcca cggataacgt gggtgatttc catatcccag tcatcgacaa
     4501 ccacacagaa gttataggtt ggggaaccat cggtacggcg aataataaga tcgtccagtt
     4561 cctggttgct gaactcgatc ggaccacgga tctgatcgtc aaaaacaaca gaaccttcct
     4621 gcgggttagc aaaacgtaca acgcacggtt catcatcagc gtgatgctca tggctgtggc
     4681 ggcagcgacc gtcataacgc ggcttctcac ctttcgccat ttgctcttcg cgcagcgctt
     4741 ccaggcgctc tttagagcaa tagcatttat aagcagtgcc ctcttccagc atctgatcga
     4801 tcaccgcgtt gtagcgatca aaacgtttgg tctggtagta cggaccttca tcccactcca
     4861 ggctcagcca gttcatgcca tccataatgg cttcgatagc ttccggcgtg gaacgctcaa
     4921 gatcggtgtc ttcaatacgc agcacgaact caccgccgtg gttacgtgca aaaagccagg
     4981 agtaaagggc agtacgcgcg ccgccaacgt gcagatagcc tgttgggctt ggcgcgaagc
     5041 gagttttgat tttcatgaaa tggccttacg tttagaaaga tgccgacaac cggcaaatcc
     5101 tggaaaaatt gagtgggcga tattctatca ctcccgcctg attcctcaat gtagttcggg
     5161 tttctacctt acgcattatt gttttcgttg acaaattgcg caacacggtc tattttgcga
     5221 tcatcttgct taaatttacg acgaacgaac aatttcttta gaaaatgcgt tgactcattt
     5281 tgaactctcc ctataatgcg actccacaca gcggggggtg attagctcag ctgggagagc
     5341 acctccctta caaggagggg gtcggcggtt cgatcccgtc atcacccacc aactacttta
     5401 tgtagtctcc gccgtgtagc aagaaattga gaagtgggtg attagctcag ctgggagagc
     5461 acctccctta caaggagggg gtcggcggtt cgatcccgtc atcacccacc actttctcac
     5521 cagctaaatt tcttgtaaca atgtgaagta ccgaagtggg tgattagctc agctgggaga
     5581 gcacctccct tacaaggagg gggtcggcgg ttcgatcccg tcatcaccca ccacttcggg
     5641 tcgttagctc agttggtaga gcagttgact tttaatcaat tggtcgcagg ttcgaatcct
     5701 gcacgaccca ccaatgtaaa aaagcgccct aaaggcgctt ttttgctatc tgcgatttgt
     5761 aagattgatt tttcttctca agttccttgt tcttctaccg atattacctc gtacagtgac
     5821 gaggactgaa caatggccac tatctcttca acttcaattc cttccataca aacacagagt
     5881 agtaacaggg catcacaagg tagcgatgtt gcttcacaaa tcgcgcggat aagtcagcaa
     5941 atcatcaaac tcactcaaca aattaaagag attgtagaca ctagcggcag tgctgaagac
     6001 aagcaaaaac aggccgaatt gattcaacag caaatcaccc tactggaaac acaactggcg
     6061 cagttgcaga aacagcaggc agaaaaggcg caggagaaag agcagcgttt gtctctggat
     6121 gtatcccttc taaatcctgt tgaaaaaacg actcatatcg atatctatat ctgatacaac
     6181 ggatcccctt cccgaccagc cattagcgca ttgccctggt ctcgtaccac gtcccataac
     6241 gcttcagcgg cggtggaaag tgaacggttc ttcctgcgca ccagcatcaa ctggcgttca
     6301 acaaccggcg taatgcgctt taccaccagt gggctacctt caggcaatgg tagcgccagt
     6361 gcgggaagaa tactgatgcc gatgcctgcg gctaccatcg ggaacagcgt cgccggatgt
     6421 ccaatctcct gcacaatatt cgcctgaata ccattgcgcg ccagcgctgc gtcaatcagc
     6481 ggtcggctgc ctgacgcgta atcctgcaac accaatttcg ctccctgtaa tgcctgccag
     6541 ggtacataat cttccacggc caaagcgcta tcgcgatggc aaagcagaaa gaaaggctcg
     6601 gaaagaatcg cttcacattg cagatcgccc actgggcctg gatcgatgac gatgccaaag
     6661 tcgacatctc cctggcgaat actttccatc acccactgct gcggacgatc gtgcaggaca
     6721 aactgaatat ctggatagcg gcgatggctt tcagcaatgc attgcggaat aagatgcgcg
     6781 gaaatggtct ggctggcagc gacccgaact tttccgctca gttgttgccc catacgcccg
     6841 gtatcgcgca acgtgctgtt aagttcatcc agcagtcgct caagacgcaa agccaactgc
     6901 tgccctgcat ctgtaagcac cacttcacgc gtggttctgt ccagcaggcg aacaccagta
     6961 tgattttcca gctccttcac actgtgactt actgccgact ggctcaagcc gatacgctct
     7021 cctgcacgac taaaactttt ctcctgcgct actgtgacga aaacctttaa ttgttttaaa
     7081 gaataattca tctgttttct tcatgaatgg atgcaataaa tcaattttat ttctcaaact
     7141 ggaagaagca caatagaacc atcgatcatc tggagtcttt atgaaacttt ttcgtatcct
     7201 cgatcctttc accttaaccc tgatcacggt ggtgttgctg gcctctttct ttccggccag
     7261 aggcgatttc gtccccttct ttgaaaatct gaccaccgca gctattgccc tgctgttctt
     7321 tatgcacggc gcgaagttgt cgcgtgaggc gattattgct ggcggtggtc actggcgact
     7381 gcatttgtgg gtaatgtgca gcaccttcgt gctgtttccg attctgggtg tactgtttgc
     7441 ctggtggaaa ccggtaaatg tcgacccgat gctctactcc ggttttctct acttgtgcac
     7501 tctcccggct accgtgcagt ctgcaatcgc cttcacgtca atggcgggcg gtaacgtcgc
     7561 ggcggcggtt tgttctgcgt cagcatccag cctgctggga attttccttt caccattgct
     7621 ggttggtctg gtgatgaatg ttcacggtgc agggggcagc cttgagcagg tcggtaaaat
     7681 tatgctgcaa ctgctgctgc cgtttgtgtt ggggcatctt tcccggccgt ggattggtga
     7741 ctgggtgtcg cgcaataaaa aatggattgc gaaaactgac cagacgtcca ttctgttggt
     7801 ggtttataca gcgttcagcg aagccgtcgt taatggtatc tggcataaag ttggctgggg
     7861 atcattgctg tttatcgtgg tggtcagctg cgttcttctg gctatcgtga ttgtagttaa
     7921 cgtctttatg gcacgccgac tgggcttcaa taaggcagat gaaattacta tcgtcttttg
     7981 tggttcgaaa aagagtctgg caaatggcat cccgatggca aacattctgt tccccacatc
     8041 ggtgatcggt atgatggtgc tgcccctgat gattttccat cagatccaat tgatggtctg
     8101 tgcggtgctg gcgcgtcgat acaaacgcca gaccgaacag ttacaggcgc agcaggaaag
     8161 cagcgccgat aaagcttaaa gcggacgctt caggggctgg accagttgcg tcagcccctc
     8221 ggttttaatc agcagcgtga tttgcattag ctcaccgagc ttccctgccg ggaactcatc
     8281 tttgcgggca aaccacagca gatactcctc cggcaggtcg attaagcgac gccctttgta
     8341 tttgccaaac ggcattatcg tattggctat ttcaatcagc tgctcttttt ccatctcagc
     8401 tacccagcaa acgcagcatt tccgcttcgt cgatgacttc aatgcccagt tcctgcgcct
     8461 tcgccagttt agatcctgca gcttcacccg caatcaccag atcggttttc ttcgacacgc
     8521 tgcccgcgac tttcgcgccc agttcgacca gtcgagcttt agcgtcatca cgcgacatct
     8581 ggcttaagct gcccgtaagc accacggttt taccagcaaa cgggctgtca atctcttccg
     8641 cgttgataac gatcggcgca ggccagtgaa caccttccgc caacagctcg ctgatgacat
     8701 tgcggttgct ttcttcggca aagaagttgt gaacgtggga tgcaacgaca atgccaacat
     8761 caggcacctt ttgcagctct tcaatcgaag cggcttccag cgcttccagc gtgccgaaat
     8821 atgccgccag acctgctgcg gtggcctcgc cgacttcacg gatgccaagt gcatagagga
     8881 agcgagcaaa ggtggtttct ttcgcttttt ccagcgcgtt aaccacgttt tgtgccgatt
     8941 ttggccccat acgctccagt ccggtcagtt tgcctgcggt gagtttgaac agatctgccg
     9001 gagtgtggac atattctttt tcaaccagct gatcgatgat tttgtcgccc attccgtcaa
     9061 catccatcgc acggcgggaa acaaagtgtt tcagcgactc tttacgctgc gcaccgcaaa
     9121 tcaggccacc ggtacagcgg gcaaccgctt caccttccac acgctcaacg tcagaaccac
     9181 ataccggaca atgcgtcggg aatacaacct cacgggtatc ttccgggcgt tcagaaagca
     9241 cgacgttaac cacctgcggg atcacgtcgc cagcgcggcg aatcaccact ttatcgccaa
     9301 tgcgtaaacc aagacgttcg atttcatccg cattgtgtaa ggttgcgtta ctcaccagca
     9361 cgccagcaac atggacaggt tccagacgcg caacaggcgt aatcgcgcca gtacgcccaa
     9421 cctgaaactc gacgtcacgc acaaaggtca tctgctcctg cgccgggaat ttaaacgcta
     9481 ccgcccagcg cggggcacgc gcgacaaagc caagctgctc ctgctgttcc agtgagttga
     9541 ccttaatcac cacgccgtcg atatcaaagc ccagcgtcgg gcggtcttct tccactttgt
     9601 gatagaacgc cagcacttct tccgccgatt cacaaagcgt tacccgatcg ctgaccggca
     9661 acccccactt tttaaattgc agtaaacggc caagatgagt atccggcagc tcgccacctt
     9721 ccagaacacc aacgccatag cagaaaaaag tgagcggtcg cttcgctgta atacgcggat
     9781 caagctgacg cagtgaacca gctgccgcat tacgtgggtt agcaaaaact ttcccgcccg
     9841 tgcgtcgcgc atcttcgtta atcttttcga accccgcctg cggcaggaac acttcaccac
     9901 gcacttccag acgcgccggg atattctctc catgcagctt cagcggaatg gcgcgaatag
     9961 tacgcacatt agacgtgata tcttccccgg tggtgccatc gccacgggtc gcggcactga
    10021 ctaaaacgcc attttcatac agaatactga cggcaaggcc atccagcttc agctcacagc
    10081 accaggtgac tttctcgttg tttttcagac ggtcctgcac acgtttgttg aaagcaagaa
    10141 agctttcttc atcaaaaacg ttatccagtg acagcattgg tacttcatgg cgtatctggc
    10201 tgaaagccgc cagcggcgca gcacctacac gttgggtagg cgaatcaggc gtaatcagtt
    10261 ctggatgttt agtttccagc tcgcgcagtt cgcgcatcag cctgtcgtat tcagcgtcgg
    10321 gaatttccgg cgcatccatc acatgataaa gatattcatg atggcgaagc gtcgttcgca
    10381 gttctgtcag ttgttgttcg attgattcca tatcgcacca tcaatgctaa aaacccccga
    10441 caagcggggg ttcgaagaag agttaatttg ccttaagtgt atcaggcgtt ggcgtctttg
    10501 acttcgcgga tgatgtcctg gtactcgcgc aatttctgcg gagtcatcat acggcgctga
    10561 tcgtcaagca cgacaccgcc cacttcatcg gcaatatgct gcgcagattg cagcatcagc
    10621 ttgaagttct gcagctcgtc accgtaagac ggtacctgca taaagatagt gacacccgga
    10681 gtagtgaaat ccttcatttc aggatcaaag gttcccggtt tcaccatatt cgccaggctg
    10741 aataacgccg ggccgctgcc atccgggcta agatgacgat ggtaaatatt catatcgcca
    10801 aaaatgaagc ccgcttgttg aatgctgtta agaagcagtt caccgtttag ctcgctaccg
    10861 tgatgcgccg cgacgttcat gataatcacc gcttctttgc gcttcggttt atccataact
    10921 ggagccggtt ccgctacagg ctcaggctgt ggtgccgcta cgggttctgc aggctggaaa
    10981 gcctgttgtg ccggttgcgg tgctgaatgc acaggctgcg gcgctggcgc gacctgtggc
    11041 gaaactggct gctgcaacgg ctgttcaggc tgcggctgat agacaggctg ctgcaccggc
    11101 tgcgctggac gcggagcatg ttgcggcggt acctgcgctt caggcggctg ctggaccggt
    11161 tgacgcggct gcgcagacgc ataaggcggt tggtactggt gttgcggcga cggacgagca
    11221 gcctcatgct cctgagcgtt agccggggca tgattcacgc ggtgaacacg aacctcacca
    11281 acgccctcat catcttcgac atcctcgtca taagaatcgt cgtcacgttt tgacttcatt
    11341 cgttttaatg gccgatcgcg gaacatagaa gatcgttctt tacggctggt ccagaaacca
    11401 tgtaccagta aagcgattat ggcgatcgcg ccaacaatga ttaatatcag acgcaaatcc
    11461 tgcatcatta tattctctgt tgttctaaca ccttgccaca acggcaaaca tttactcact
    11521 aagagtattt gccgattacc tcaagtgcaa gtgcactatt aactttcaca gcacaaagat
    11581 agatgaaatc gtgctttttg ctgttttttc gaacatatcc taactgtcca ttgcgcaatt
    11641 acccggtaaa atacgcagaa ttttcctagg attggtcaaa aggagctcat cctgattatg
    11701 gtttcatcat tcacatctgc cccacgcagc ggtttttact attttgcgca aggctggaag
    11761 ctcgtctcgc aacctgggat tcggcgtttc gttattttac cgctgctggt caatattttg
    11821 ttgatggggg gcgcattctg gtggctcttt acacagctcg atgtctggat cccgactctc
    11881 atgagttacg ttccggactg gctacaatgg ctgagttatc tgttgtggcc tctggctgtc
    11941 atctctgtgc tgttagtgtt tggctatttc ttctccacga ttgctaactg gattgccgct
    12001 ccgtttaacg gtttattggc tgaacaactg gaagcacgat tgactggcgc tacaccgcca
    12061 gataccggga ttttcggtat catgaaagat gtgccgcgaa tcatgaaacg cgaatggcaa
    12121 aaatttgcct ggtatctgcc gcgcgcaatt gtattactaa ttctttactt catccctggt
    12181 attgggcaaa ccgtcgcgcc ggtactgtgg ttcctgttta gcgcctggat gttagccatc
    12241 cagtattgcg attacccctt cgataaccac aaagtgccgt ttaaagagat gcgcaccgcc
    12301 ctgcgcacac gcaaaatcac caatatgcag tttggtgctt taaccagcct gtttacgatg
    12361 atcccgctgc ttaatctgtt catcatgccc gttgccgttt gtggcgcgac ggcgatgtgg
    12421 gtcgattgct atcgcgataa acacgcgatg tggcggtaac aatctaccgg ttattttgta
    12481 aaccgtttgt gtgaaacagg ggtggcttat gccgcccctt attccatctt gcatgtcatt
    12541 atttcccttc tgtatataga tatgctaaat ccttacttcc gcatattctc tgagcgggta
    12601 tgctacctgt tgtatcccaa tttcatacag ttaaggacag gccatgagta agatttttga
    12661 agataactcg ctgactatcg gtcacacgcc gctggttcgc ctgaatcgca tcggtaacgg
    12721 acgcattctg gcgaaggtgg aatctcgtaa ccccagcttc agcgttaagt gccgtatcgg
    12781 tgccaacatg atttgggatg ccgaaaagcg cggcgtgctg aaaccaggcg ttgaactggt
    12841 tgaaccgacc agcggtaata ccgggattgc actggcctat gtagctgccg ctcgcggtta
    12901 caaactcacc ctgaccatgc cagaaaccat gagtattgaa cgccgcaagc tgctgaaagc
    12961 gttaggtgca aacctggtgc tgacggaagg tgctaaaggc atgaaaggcg caatccaaaa
    13021 agcagaagaa attgtcgcca gcaatccaga gaaatacctg ctgctgcaac aattcagcaa
    13081 tccggcaaac cctgaaattc acgaaaaaac caccggtccg gagatctggg aagataccga
    13141 cggtcaggtt gatgtattta ttgctggcgt tgggactggc ggtacgctga ctggcgtcag
    13201 ccgctacatt aaaggcacca aaggcaagac cgatcttatc tctgtcgccg ttgagccaac
    13261 cgattctcca gttatcgccc aggcgctggc aggtgaagag attaaacctg gcccgcataa
    13321 aattcagggt attggcgctg gttttatccc ggctaacctc gatctcaagc tggtcgataa
    13381 agtcattggc atcaccaatg aagaagccat ttctaccgcc cgtcgtctaa tggaagaaga
    13441 aggtattctt gcaggtatct cttctggagc agctgttgcc gcggcgttga aactacaaga
    13501 agatgaaagc tttaccaaca agaatattgt ggttattcta ccatcatcgg gtgagcgtta
    13561 tttaagcacc gcattgtttg ccgatctctt cactgagaaa gaattgcaac agtaatgcca
    13621 gcttgttaaa aatgcgtaaa aaagcacctt tttaggtgct tttttgtggc ctgcttcaaa
    13681 ctttcgcccc tcctggcatt gattcagcct gtcggaactg gtatttaacc agactaatta
    13741 ttttgatgcg cgaaattaat cgttacagga aaagccaaag ctgaatcgat tttatgattt
    13801 ggttcaattc ttcctttagc ggcataatgt ttaatgacgt acgaaacgtc agcggtcaac
    13861 acccgccagc aatggactgt attgcgctct tcgtgcgtcg cgtctattaa aaactggcgc
    13921 taacaataca ggctaaagtc gaaccgccag gctagacttt agttccacaa cactaaacct
    13981 ataagttggg gaaatacaat gttccagcaa gaagttacca ttacagctcc gaacggtctg
    14041 cacacccgcc ctgctgccca gtttgtaaaa gaagctaagg gcttcacttc tgaaattact
    14101 gtgacttcca acggcaaaag cgccagcgcg aaaagcctgt ttaaactgca gactctgggc
    14161 ctgactcaag gtaccgttgt gactatctcc gcagaaggcg aagacgagca aaaagcggtt
    14221 gaacatctgg ttaaactgat ggcggaactc gagtaatttc ccgggttctt ttaaaaatca
    14281 gtcacaagta aggtagggtt atgatttcag gcattttagc atccccgggt atcgctttcg
    14341 gtaaagctct gcttctgaaa gaagacgaaa ttgtcattga ccggaaaaaa atttctgccg
    14401 accaggttga tcaggaagtt gaacgttttc tgagcggtcg tgccaaggca tcagcccagc
    14461 tggaaacgat caaaacgaaa gctggtgaaa cgttcggtga agaaaaagaa gccatctttg
    14521 aagggcatat tatgctgctc gaagatgagg agctggagca ggaaatcata gccctgatta
    14581 aagataaaca catgacagct gacgcagctg ctcatgaagt tatcgaaggt caggcttctg
    14641 ccctggaaga gctggatgat gaatacctga aagaacgtgc ggctgacgta cgtgatatcg
    14701 gtaagcgcct gctgcgcaac atcctgggcc tgaagattat cgacctgagc gccattcagg
    14761 atgaagtcat tctggttgcc gctgacctga cgccgtccga aaccgcacag ctgaacctga
    14821 agaaggtgct gggtttcatc accgacgcgg gtggccgtac ttcccacacc tctatcatgg
    14881 cgcgttctct ggaactgcct gctatcgtgg gtaccggtag cgtcacctct caggtgaaaa
    14941 atgacgacta tctgattctg gatgccgtaa ataatcaggt ttacgtcaat ccaaccaacg
    15001 aagttattga taaaatgcgt gctgttcagg agcaagtggc ttctgaaaaa gcagagcttg
    15061 ctaaactgaa agatctgcca gctattacgc tggacggtca ccaggtagaa gtatgcgcta
    15121 acattggtac ggttcgtgac gttgaaggtg cagagcgtaa cggcgctgaa ggcgttggtc
    15181 tgtatcgtac tgagttccta ttcatggacc gcgacgcgct gcccactgaa gaagaacagt
    15241 ttgctgctta caaagcagtg gctgaagcgt gtggctcgca ggcggttatc gttcgtacca
    15301 tggacatcgg cggcgacaaa gagctgccat acatgaactt cccgaaagaa gagaacccgt
    15361 tcctcggctg gcgcgctatc cgtatcgcga tggatcgtaa agagatcctg cgcgatcaac
    15421 tccgcgctat cctgcgtgcc tcggctttcg gtaaattgcg cattatgttc ccgatgatca
    15481 tctctgttga agaagtgcgt gcactgcgca aagagatcga aatctacaaa caggaactgc
    15541 gcgacgaagg taaagcgttt gacgagtcaa ttgaaatcgg cgtaatggtg gaaacaccgg
    15601 ctgccgcaac aattgcacgt catttagcca aagaagttga tttctttagt atcggcacca
    15661 atgatttaac gcagtacact ctggcagttg accgtggtaa tgatatgatt tcacaccttt
    15721 accagccaat gtcaccgtcc gtgctgaact tgatcaagca agttattgat gcttctcatg
    15781 ctgaaggcaa atggactggc atgtgtggtg agcttgctgg cgatgaacgt gctacacttc
    15841 tgttgctggg gatgggtctg gacgaattct ctatgagcgc catttctatc ccgcgcatta
    15901 agaagattat ccgtaacacg aacttcgaag atgcgaaggt gttagcagag caggctcttg
    15961 ctcaaccgac aacggacgag ttaatgacgc tggttaacaa gttcattgaa gaaaaaacaa
    16021 tctgctaatc cacgagatgc ggcccaattt actgcttagg agaagatcat gggtttgttc
    16081 gataaactga aatctctggt ttccgacgac aagaaggata ccggaactat tgagatcatt
    16141 gctccgctct ctggcgagat cgtcaatatc gaagacgtgc cggatgtcgt ttttgcggaa
    16201 aaaatcgttg gtgatggtat tgctatcaaa ccaacgggta acaaaatggt cgcgcctgta
    16261 gacggtacca ttggtaaaat ctttgaaacc aatcacgcgt tctctatcga atctgatagc
    16321 ggcgttgaac tgttcgtcca cttcggtatc gacaccgttg aactgaaagg cgaaggcttc
    16381 aagcgtattg cagaagaagg tcagcgcgtg aaagttggcg atactgtcat tgaatttgat
    16441 ctgccgctgc tggaagagaa agccaagtct accctgactc cggttgttat ctccaacatg
    16501 gacgaaatca aagaactgat caaactgccc ggtagcgtaa ccgtgggtga aaccccggtt
    16561 atccgcatca agaagtaatt cttgccgcag tgaaaaatgg cgccca
//
LOCUS       DAZPEB010000082        16540 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZPEB010000082 DAZPEB010000000
VERSION     DAZPEB010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16540
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.083"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(59..223)
                     /gene="rhuM"
                     /locus_tag="ACHURJ_002284"
                     /pseudo
     CDS             complement(59..223)
                     /gene="rhuM"
                     /locus_tag="ACHURJ_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774140.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
     gene            complement(302..396)
                     /locus_tag="ACHURJ_002285"
     tRNA            complement(302..396)
                     /locus_tag="ACHURJ_002285"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(360..362),aa:Sec,seq:tca)
     gene            689..2071
                     /locus_tag="ACHURJ_002286"
     CDS             689..2071
                     /locus_tag="ACHURJ_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFR1281216.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVLGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            2081..4372
                     /gene="yicI"
                     /locus_tag="ACHURJ_002287"
     CDS             2081..4372
                     /gene="yicI"
                     /locus_tag="ACHURJ_002287"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFR1281217.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGQDTNNQRNYMFERLDLGVGETVYGLGERF
                     TALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQSVSFEVGSEKVSK
                     VQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTSFTTNYDEAT
                     VNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRRLKAKGLKIC
                     VWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNPDACKWYADK
                     LKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAHIYNELVWNVLKDTVGEE
                     EAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFWSHYIGGFEN
                     TAPAHVYKCWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLKCRMMPYLYR
                     EAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAGDVQFYLPEG
                     RWTHLWHNDELDGRCWHKQQHSFLSLPIYVRDNTLLALGNNDQRPDYAWHEGTAFHLF
                     NLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRNVVKVNGLQD
                     GSQAESEQGLVVKPQGNALTITL"
     gene            complement(4425..6134)
                     /locus_tag="ACHURJ_002288"
     CDS             complement(4425..6134)
                     /locus_tag="ACHURJ_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFR1281218.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPKAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDAHLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLLMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTTLDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGIVSSGEVTSAE
                     LVQ"
     gene            complement(6255..7646)
                     /gene="xanP"
                     /locus_tag="ACHURJ_002289"
     CDS             complement(6255..7646)
                     /gene="xanP"
                     /locus_tag="ACHURJ_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFR1281219.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKTWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITSTSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            7926..9131
                     /gene="gltS"
                     /locus_tag="ACHURJ_002290"
     CDS             7926..9131
                     /gene="gltS"
                     /locus_tag="ACHURJ_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFR1281220.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            complement(9165..11246)
                     /gene="recG"
                     /locus_tag="ACHURJ_002291"
     CDS             complement(9165..11246)
                     /gene="recG"
                     /locus_tag="ACHURJ_002291"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFR1281221.1"
                     /translation="MTGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(11252..11941)
                     /gene="trmH"
                     /locus_tag="ACHURJ_002292"
     CDS             complement(11252..11941)
                     /gene="trmH"
                     /locus_tag="ACHURJ_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFR1281222.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(11948..14056)
                     /gene="spoT"
                     /locus_tag="ACHURJ_002293"
     CDS             complement(11948..14056)
                     /gene="spoT"
                     /locus_tag="ACHURJ_002293"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFR1281223.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(14075..14350)
                     /gene="rpoZ"
                     /locus_tag="ACHURJ_002294"
     CDS             complement(14075..14350)
                     /gene="rpoZ"
                     /locus_tag="ACHURJ_002294"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFR1281224.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(14405..15028)
                     /gene="gmk"
                     /locus_tag="ACHURJ_002295"
     CDS             complement(14405..15028)
                     /gene="gmk"
                     /locus_tag="ACHURJ_002295"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFR1281225.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     DEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            15286..>16540
                     /gene="ligB"
                     /locus_tag="ACHURJ_002296"
     CDS             15286..>16540
                     /gene="ligB"
                     /locus_tag="ACHURJ_002296"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFR1281226.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEIFRQQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMSDRLKELATAGFTLTQTYTRAVKNADEVARVRNEWWKAELPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     SVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLV"
BASE COUNT         3756 a         4228 c         4495 g         4061 t
ORIGIN      
        1 aatagtcgta taaaaaaggg tgttatggag cgtgtaaaac aacatcttca ccttaatggc
       61 gatacagttg gctgagagcc agcagggggc aaatcacctg caaataaaca cggcacatgc
      121 tggcgcgcat atcgcggatg cgttcgagga cagaagaacc gacaggcgga ttcttcagcc
      181 gctcatcgtc catcacaaaa ccttagacca ggtattcctg aagccgattg aatattgagc
      241 agtagaaaat atctggattg acagtattac aaggggttgg agaacgtcag aagaggcatt
      301 ttggcggaag atcacaggag tcgaacctgt ccgggaccgc tggcggcccc aactggattt
      361 gaagtccagc cgcctcaccg gagacgacga tcttccgcgc ctcgattgct acatggaggc
      421 ggggcgcatt atagctactt ccttgagttt ctacatcccc cagatcagtt ttttccgcct
      481 aatacacgca gcgtattatc acgaaagcat tatagttcat ataaaaccca tattttacaa
      541 tgacatatga gcggaattaa cgctaccacg atcacataaa acaagaatca taaattaata
      601 accagatatc ggaatattcg ctctcacagg gatggtttac aaaatgcgtt atcggtctca
      661 gcacccctat agcatcaagg aaaagcagat gaagagtgaa gtgttgtccg ttaaagagaa
      721 aattggttat ggcatgggag acgccgccag ccacattatt ttcgataacg taatgttata
      781 tatgatgttc ttttataccg atatttttgg cattcctgcc ggttttgtcg gaaccatgtt
      841 tttggtcgct cgtgcactgg atgcgatttc cgatccttgc atggggttgt tggccgatcg
      901 aacgcgctct cgctggggta aatttcgtcc gtgggtactg tttggcgcac tgccattcgg
      961 gatcgtctgt gtactggcct atagcacgcc agatctcagt atgaacggca aaatgatcta
     1021 tgcagcaatt acttacaccc tacttacctt actttatacc gtcgtcaata tcccttactg
     1081 cgcattgggt ggtgtaatca ccaatgaccc gactcagcgt atctcgctgc aatcctggcg
     1141 ttttgtgctg gcgaccgcgg gaggcatgct ttctactgtt ctgatgatgc cactggttaa
     1201 tttaattggc ggtgataata aaccactcgg tttccagggc ggtatcgcgg tcctttccgt
     1261 ggtggcattc atgatgctgg cattttgttt cttcaccact aaagaacgcg ttgaagcacc
     1321 acctacaaca acgtctatgc gggaagattt acgtgatatc tggcaaaacg accagtggcg
     1381 gattgtcggt ttactaacca ttttcaatat cctggcggtg tgcgtactcg gtggggcgat
     1441 gatgtattac gtcacatgga ttttgggcac gccggaagtg tttgtcgctt ttctcaccac
     1501 ttattgcgtg ggtaacctga ttggttccgc actggcaaaa cctctgaccg actggaaatg
     1561 taaagtcact atcttctggt ggacgaacgc cctgctggca gtgattagcc tcgcgatgtt
     1621 ctttgttccc atgcaggcca gcatcactat gtttgtcttc atcttcgtga ttggtgtgtt
     1681 gcatcaactg gtgacaccta tccagtgggt aatgatgtcc gataccgtcg actacggcga
     1741 gtggtgcaat ggtaaacgcc tgaccgggat cagttttgct ggcacgctgt ttgtgctcaa
     1801 actggggttg gccttcggcg gcgctcttat cggctggatg ctggcttatg gcggatatga
     1861 tgcggcagaa aaagcgcaga acagcgccac gattagcatc attattgcgc tattcacgat
     1921 tgttccggcg atctgttatt tgctgagcgc gattatcgct aaacgctact actcactcac
     1981 gacgcacaat ctgaaaaccg ttatggaaca gctggctcag ggtaaacgcc gttgccagca
     2041 acaattcacc tctcaagaag tgcagaacta aggaacggca atgaaaatta gcgatggaaa
     2101 ctggttgatt caacctggcc tcaatttgat tcacccgctt caggtgttcg aggttgaaca
     2161 gcaggataat gaaatggtgg tctatgctgc cccccgtgat gtgcgtgaac gtacctggca
     2221 gcttgatacg cctttattta cgctgcgctt tttctcccca caggaaggta ttgtcggtgt
     2281 gcggattgag cattttcagg gagcgctgaa taacggtcct cattatccgc tcaatatttt
     2341 gcaggacgtg aaggtcacaa tcgaaaacac agaacgttat gctgagttta aaagtggcaa
     2401 cttaagcgcg cgtgtcagca aaggtgagtt ctggtcactc gattttctgc gcaacggtga
     2461 acgtattacc ggtcaggaca cgaataatca acgaaattat atgtttgagc ggcttgatct
     2521 tggcgttggc gaaacagttt atggtctggg agagcgtttt actgccctgg tgcgcaatgg
     2581 tcagacggta gagacctgga accgggacgg cggcacaagt actgaacagg cgtataaaaa
     2641 tatcccgttc tatatgacta accgtggcta tggggtgctg gtcaatcacc cccagagtgt
     2701 ctcttttgaa gtgggatcgg agaaagtctc caaagtgcag ttcagcgttg agagcgaata
     2761 tctcgaatac tttgttatcg acggtccgac gccgaaagcg gtacttgatc gttatacccg
     2821 ttttactggt cgtccggcgc tgccgcccgc atggtccttc ggcctgtggc taaccacttc
     2881 atttaccacc aactacgacg aagcgacggt aaacagcttt atcgatggta tggcggaacg
     2941 caatctgccg ctgcatgttt tccactttga ctgtttctgg atgaaagcct tccagtggtg
     3001 cgattttgag tgggacccgc tgactttccc ggacccggaa gggatgatcc gccgcctgaa
     3061 agcgaaaggg ctgaaaatct gcgtctggat taacccctat atcggtcaaa aatcccccgt
     3121 ctttaaagag ttacaagaga aaggctattt actcaaacgc ccggacggtt cgctatggca
     3181 gtgggataaa tggcagccag gtctggcgat ttatgacttt accaatccgg atgcctgcaa
     3241 atggtacgcc gacaaactga aaggtctggt cgcgatgggc gttgattgct ttaagaccga
     3301 ctttggcgaa cgtatcccaa ctgatgttca gtggtttgac ggttccgatc cgcagaaaat
     3361 gcataaccat tatgcgcaca tctacaacga actggtgtgg aacgtgctca aggacaccgt
     3421 tggtgaggaa gaagctgtct tgtttgcccg ctcggcctcc gtcggtgcgc agaaattccc
     3481 ggtacactgg ggtggcgatt gttacgctaa ctacgaatca atggcggaaa gcctgcgcgg
     3541 tggtttgtct attggccttt caggttttgg cttctggagc cactatatcg gcggctttga
     3601 aaataccgct ccggcgcacg tttacaaatg ctggtgcgcg tttggtttgc tctccagcca
     3661 tagccgttta cacggtagca aatcttatcg tgtgccgtgg gcctacgatg atgagtcctg
     3721 tgatgtggtg cgcttcttca cgcaactgaa atgccgcatg atgccgtatc tgtatcgtga
     3781 agctgcgcgt gcaaacgcgc ggggtacgcc gatgatgcgg gccatgatga tggagttccc
     3841 ggacgatccg gcttgtgatt accttgaccg tcaatacatg ttaggcgaca acgtgatggt
     3901 tgcgccggtg ttcactgaag cgggcgatgt gcagttctac ctgccggaag gtcgctggac
     3961 acacctgtgg cacaacgatg agctcgacgg tcgttgctgg cataaacagc agcacagctt
     4021 cctgagtctg cccatttatg tgcgtgataa caccctactg gcgctgggca acaacgatca
     4081 acgtcccgat tacgcgtggc acgaaggcac ggcattccac ctctttaatc tgcaagacgg
     4141 gcatgaagcc gtctgtgaag tgcccgctgc tgacggatcg gtgatcttta ctttaaaagc
     4201 agcacgtact ggcaacacga ttactgtgac tggtgcgggc gaggcgaaga actggacact
     4261 gtgcctgcgc aatgttgtga aagtaaatgg tctgcaagac ggttcgcagg ctgaaagtga
     4321 gcaggggctg gtggtgaagc ctcaagggaa tgcgctgaca attacgttgt aatctattga
     4381 tggaatgccg ggtacgtctg agtcgtaccc ggcgagattg atgtttactg caccagctct
     4441 gccgacgtaa cttcaccgct ggaaacgatg ccagcattca tggtttgagt cacttgctgc
     4501 tttgcggcgt tcacggcatg caattgcccg ctaaccgtag gtttcagcgg ggcattggcc
     4561 tgaatatctc cactggcggt aagctgaata ttgccgtcgc cagtcaacgg taattcaggc
     4621 catccccatt gttgcaaaat attcaccggc acaccgcgtc cattcaggct gatatgggtc
     4681 tgacgttgtg gcgtttgtga aacactggca gtggcttcca gaatgccttt ttcagtaaat
     4741 gcactcagtt cgctgatatt caccgtgctg ctgttggcgg tcagcgccag cgacgggcga
     4801 cgaacatcaa cacgattgaa tgtcgcggcg gcggcattca gattcgccga gccactccag
     4861 acgccccatt tatgatcggt aaccagcgtc aggttggcac cgtaaccatc gagcgtggtg
     4921 agctgccacg ggaagtcagg gtcgatatca atgatcagat tgcggctggc gctaaatctc
     4981 ttcagttgca ggctgtttaa ccaaccgggt gtcgtttcca tccacaactg ttgccagttt
     5041 ttcggcaagg tatattccag cccggcaatt gccgcatcat caaggatcaa cgttttcccg
     5101 tcacgcagcc agttccctga cgttctgacc ataccccctt cccagcggct ggtgaactgg
     5161 cgcagcgcta cgccctgcgg ggaaaattcc gcgtttataa tcgggtcaaa taaatgcagc
     5221 gaaccataaa tgaactcgct agcgttcatc aacagtttgc cttcttgtgt ctgccagtca
     5281 tctttactga aggtcatgtt gcgcaagctg agatcgaggt cggtcaccgc ccagtccgga
     5341 ccttgcaaat gagcatcgat cacttccagg cgaccaatct gcaacgacgg gacagagcgt
     5401 aatggcgcaa agaagtcggt tagcgatttt tcgctttgta gacgtatatc cgccatgcgc
     5461 aggttttcca cttgccagct gccgtcggcg ttacgctgcg cgtttccggt taatgtcccg
     5521 cgggcgatgt cggcacccag gttagtcagc gtaacgcgat cgttatcaat actgccttca
     5581 atcagtacat tggtggcagg aacatcattg agcgaaagcg atccggcact aaactgaatc
     5641 tgcgccttcg tacccagcac tttaccggct tttggtgacc acggaactac gccgccattt
     5701 acccgctgcg cgctcagttt ccattcgcta ttcgggctat taaacgccat atcacgcagt
     5761 tgcagacgat cggctttgaa cggtagcggc gcggtctggt cggtgagatt cagcgtcccg
     5821 ttttccagca ggatggtatc gacatggcgt ggttcggtca gttgccgact gcttagcgca
     5881 atgtcgacac ttttggccac cagggtcgcg ggctgaccat cacgaccaaa cgtgacattc
     5941 tccagcacga tatgagatgg cgcggaaaaa cggtgatcca tcgccccgaa ggccagatga
     6001 tagtcgctat tctcggaaac ccatgcgctg atatgttctg ctccccagcg ggtttgcaga
     6061 agaaaataga ggccagcgat caccactaac agagcgatga gaatgtagag aagcagcttc
     6121 ccaataaatt tcatggtctt ccattccgtg aaatgcacat aagagagtta tgcacgattt
     6181 acgcgcaatc ctcaaggcgg gaatggtgaa agaggtgaga ctgcggcggt aattatctta
     6241 ccgccgccag tgaattactg tttttctggt gggaaaatca gattcagcac gatggcagta
     6301 ataccgcccg cggcgatccc ggaggagagc aggtttttca gccattcagg ggcaaactgc
     6361 aaaatcagcg gctgctgaga cacgcccaga ccaaccgcca gcgacagcgc gataatcaga
     6421 atcgcccgac ggttcagcgg ctcacgagaa acgatacgca caccggaggc ggcgatggtg
     6481 ccaaacatta caagcgttgc gccgcccaga accggttctg gaatgtgttg tacaaaaccg
     6541 ctcactgccg ggaacagacc cagcacgatc aacatcagcg cgacgacaaa accgacatag
     6601 cggctggcaa caccagtcaa ctggatcact ccgttgtttt gcccgaagca ggagttcggg
     6661 aaggtgttaa acaccgccga aacaaacgag ttcaggccgt ttgccagcac gccgcctttc
     6721 aggcgtttca tgtacagcgg accggacact ggctgttcgg aaacgtcaga ggtcgacgtg
     6781 atatcgccaa tggtttccag cgaagtgatc ataaagacca gcatcagcgg cagcagcaga
     6841 ctccattcaa tgccaagacc gtaatagagc ggcgttggca ccataatcag ttcttgcgtc
     6901 atcggttcgt tgctttctgg caacatgccc ataaaccacg ccagcgcata tccggccgcc
     6961 atcgcaatca ccagtgaggc cacgcgtaag taagggttac gttgacggtt aagcaggata
     7021 attaaggcta agaccacgcc tgccagcagc agatttttcg gtgcgccgaa ggtgttatcg
     7081 ctcatggctg cgtaaccgcc gccaatggac gttaacccaa cctgaattag cgacaggccg
     7141 ataatcatca ccacaacgcc agaaaccagc ggcgtaataa tgcggcgcgc cagatgcaga
     7201 acgcgggaga tcaccatctc ggtgcaactt gccagcatca acgtgccgaa caaagccgcc
     7261 atcatggtag gaacatcagc accaccggtt ttcagcgcgg taccgcccat aatcagcggg
     7321 gcaacaaagt tgaagctggt gccctgaata gacaacagcc cggagccaac cggaccccag
     7381 gtcttaattt gaataatcga tgccacaccg gaggcaaaca gcgacatact aataatgtgt
     7441 tgcgtgtctt gtgccggtaa acccagcgcc tggcagatta atagcgctgg cgtgatcacc
     7501 gcaacgaaca tcgccagcag atgctgacag gcggcaaaca gggtttgagg aagcggcgga
     7561 cgatcttcaa gacggtaaat cagttcgctg ttttgagtct gcgcaaccgg ttgcgcattt
     7621 tctgactcga gggtggaaac agacatcgaa aacaatcccg tggtagcaaa gcgggcattt
     7681 tagctgaccg aaagagaaaa gcaaacgttt gccacatcct catctaaata tttaaatgag
     7741 ataaatgcac ttttttatat aacttttggt atttttctgc ttaaaatcca tgccatttgt
     7801 tcattaacag ctgagaattt gacccaggaa agcataactc gatacccgtc atagttcatg
     7861 aagttgccac tgcgcgatga agtatgacga gtatgaaaga gtgatgcgga tacaaaggag
     7921 taactatgtt tcatctcgat actttagcaa cgcttgttgc cgcaacgctg acgttgctgc
     7981 tcgggcgtaa gttggtccat tccgtctcct ttttgaagaa atacaccata ccggaacctg
     8041 ttgcgggtgg tttgttggtg gcgctggcgc tactagtact gaaaaaaagc atgggctggg
     8101 aagtcaactt tgatatgtcc ctgcgcgatc cgttaatgct ggctttcttc gccaccattg
     8161 gcctgaacgc caacattgcc agtttgcgtg ccggtgggcg tgtggttggc atcttcttga
     8221 ttgtggttgt tggtctgttg gtgatgcaaa atgccattgg cattggtatg gctagcctgt
     8281 tagggcttga tccgctgatg gggctgttgg ccggttctat tacgctttcc ggcggtcacg
     8341 gtacgggcgc tgcgtggagt aaattgttca ttgaacgtta tggcttcacc aatgcgacgg
     8401 aagtggcgat ggcctgtgca acgttcggtc tggtgctggg cggcttgatt ggcggtccgg
     8461 tggcgcgcta tctggtgaaa cactccacca cgccgaacgg tattccggat gaccaggaag
     8521 tcccgacggc gtttgaaaag ccggatgtgg gacgcatgat cacctcgttg gtgctgattg
     8581 aaactatcgc gctgattgct atctgcctga cggtggggaa aattgttgcg caacttttgg
     8641 ctggcactgc ttttgaactg ccgaccttcg tctgtgtact gtttgttggc gtgattctga
     8701 gcaacggtct gtcaataatg ggcttttacc gcgtctttga gcgtgcggta tccgtgctgg
     8761 gtaacgtaag cttgtcgttg ttcctggcga tggcgttgat ggggctgaaa ctgtgggagc
     8821 tggcttcgct ggcgctgccg atgctggcga ttctggtggt acagaccatc ttcatggcgt
     8881 tgtatgccat cttcgttacc tggcgcatga tgggcaaaaa ctacgatgcg gcagtgctgg
     8941 ctgcgggtca ctgtggtttt ggcctcggtg caacgccaac ggcaatcgcc aacatgcagg
     9001 cgatcactga acgctttggc ccgtcgcaca tggcgttttt ggtggtgccg atggtcggtg
     9061 cgttctttat cgatatcgtc aatgcgctgg taattaagtt gtatttgatg ttgccgattt
     9121 ttgccggtta accgatgaag cggcggtctt ctcaccgccg ccttttacgc attcgagtaa
     9181 cgttctgtct ccggcatcca gcgttctatc aaggcttttg cctgttgtgg gtaacgttcg
     9241 tgaatatgtc gcgccaggcg ctgaacttcc gggatcatcg cctgatcgcg cagtaaatcc
     9301 gccactttaa attcagcatt acccgtctga cgcgtgccta acaattcgcc agggccgcga
     9361 atctccagat ctttttgcgc aatcacaaaa ccgtcgttac tgtcgcgcag cacttgcagg
     9421 cgaatttgcg ccgttttaga aagcggcgtt ttgtagagca gcacgcagtg agaagccacc
     9481 gcgccacgac ctacgcgccc gcgcagctgg tgtaactgcg ccagacccag acgctcaggg
     9541 ttttcgataa tcatcaggct ggcgttaggc acatcaacac cgacttcaat aacggtagtg
     9601 gcaaccagca gatgtagctc accttgttta aacgacgcca tcaccgcctg tttctcggct
     9661 ggtttcatcc gcccgtgtac caggccaaca ttcaattctg gtagcgccag tttcaactct
     9721 tcccaggtag cttccgccgc ctgcgcttcc agtaactctg actcttcaat caacgtacaa
     9781 acccagtacg cctgacgacc ttcagttatg caggcatggc gcacgcggtc aatgatgtcg
     9841 gtacggcggg tatcaggaat agcgaccgta gtcactggcg tgcggcctgg cggcagctca
     9901 tctatcaccg aggtatcgag atcggcatac gccgtcattg ccagcgtgcg ggggatcggc
     9961 gtggcggtca tgatcaactg atgcggatgg aagccttgct gctggccttt ctcccataac
    10021 gccagacgct gatgcacgcc aaagcgatgt tgttcgtcga taatcaccag cgccagaccg
    10081 ttaaactgca cctgttcctg gaagatggcg tgcgtaccaa caatcatctg cacctgaccg
    10141 ctggcgatgg cttcctgctg agacagtcgc gctttgcctt tctgcttacc ggcgagccag
    10201 cccacttcga taccgagcgg ttcaaaccag ttgcggaagt tattggcgtg ctgttcggcg
    10261 agtaattcgg ttggtgccat caatgccacc tgtttgccgt gggcaatcgc acgcagcgcg
    10321 gcgagggcgg cgaccagcgt tttaccggaa cctacatcgc cctgtaccag gcgcatcatc
    10381 ggcacatcca gcgccatatc gcgctcgatc tccgccacta cgcgtgcctg tgcgcccgtt
    10441 ggcttgaacg gtaaggctgc gaggagttta tttttcagcg catcattggc gctcagcggc
    10501 tgggcatgaa aacgctgtgc tccggcacgt aaggctaaca tgctgaggtt gtgcgccagc
    10561 agttcttcca gaatcagacg acgttgcgcc ggatgctgcc cggtttccag atcgctaagc
    10621 tgtagcgtcg gtggcgggcg gtgcaaagtg cgcaacgctt ccggtagcgt catcattcct
    10681 tgtgacagtt ccggcggcag gagttcttca atggcgcagg tgtcgagcag atccagcgcc
    10741 tggtcggtta atttacgcag cgtggcctgc tttacgcctt ccgttgttgg ataaaccggc
    10801 gtgagcgttt cctgtaattc tggcgtgctg agatcgccct gcacgcggta ttccgggtgg
    10861 atcatctccg caccatattt accgcgcttt gcttcgccat aagccagtac acggcggccc
    10921 gtcgccaggc tatttttcat tgccgcgctg aaattgaaaa agcgcatggt gaggatgccg
    10981 gaaccgtcgc tgatctggca ggtcatcatc cgccgaccgc cgaaggagat attgcagttc
    11041 agcacttcgc cttccaccgt ggcataaacg cccggcagta gttctccgat ggggtagaga
    11101 tgggtgcgat cttcgtagcg caggggaagg tgtaagagta gatcctgcac ggtatgcagg
    11161 ttgattttcg ccagtttgtt actaagtgct gcgccaacgc ccgttaggga actgagtggg
    11221 acagcatcta acaggcgacc tgtcatggca cttaccctgc agcctgcata gtagcccacc
    11281 agtcggcatc agcttcgatc tcgccttgct gattgacgtg gggataaggc aggcctttgc
    11341 gttttgcgac tttcgccagc accggatagc cgccttcaaa caacaggcgt tgttgctctg
    11401 cttccggcaa catgctgttt tcacgcaggt acatgcctgc attttgccgc tgacgctggg
    11461 cttcgtaaag aatgagggct gaggcaacgg aaacattcag cgactgcacc atgccgatca
    11521 tcggaatgat gatgtcctga tccgccaggg ccaatgcttc ctgcgtgatg cccgttttct
    11581 cctgtcccat caaaatgcag gtcgggcgag tgtaatcaat ttcgcggaaa tcgacagcgt
    11641 tatcagaaag atgggttgcc agaatctgca tgccctggcc tttgagatga gcgacggcat
    11701 cgccaatggt gcggtgtgtt ttcacctgta cccagctgtt actacccgcc gctgccgaag
    11761 ccatggtgcg catgcggcta ccaggccaga cggcgtgaac ttcatgtacg ccaacggcat
    11821 ctgcggtacg aataatcgca gaaacgttat gaggtttgtg gacctgctcc atgcagacgg
    11881 tcagatcagg ctgccgcctg gcgagcattt cgcagatgcg tgcataacgt gttgggttca
    11941 taaaacatta atttcggttt cgggtgactt taatcacgtc tggcatcacg cggattttgc
    12001 gcatgatatt cgccagatgc acacggtcac gagcggtcag acgaataaag gcgctgtaga
    12061 cgcgaccatc tttctcttcc gtattcaaac tttgaatatt cgaagtcgtg gtgttaattg
    12121 ccgccgtcag gtttgccagc gcaccctgat gattgaacat ctccaccttg atttcggtga
    12181 tgaactcctg cgccgtctct ttatcccatt ccacagccat aaacttctct ggctctttct
    12241 ggtagccacg gatgttacgg caggattcat ggtggatcac cagaccttta ccggggctga
    12301 cgtgggcgat aatcgggtcg ccaggaatag ggcggcagca tttcgcaaag gtgatcagca
    12361 cgccatccgc gcctttgatc ggcagatgcc cgtggctttg agtcgccggt ggaatggagg
    12421 cgtccccatg ttgtagattt ttcgcgacca ccacgctcat tgcgttacca aggccgattt
    12481 ctgccagcag atcgtcaagc gttgccagct tcatgcgatc cagctcgcgc tgaatatttt
    12541 cctgcgggat ttcattgagc ttacggctac cacccaaagc atggttgagc agacgacggc
    12601 ccaggcttac agaatcatca cgcttgaggt ttttcagcaa ctgacgaatt ttggcgcgcg
    12661 ctttcgagct aacgacaaag ttcagccaag cggcattcgg gcgagcgccc ggagcggtaa
    12721 tgatttcaac ggtctgaccg ctagtaagcg gctgcgacag cgggtaaggc tggcggtcga
    12781 cgcgtgcgcc cacgcaggca tggccgatat cggtatgcac tgcataagcg aagtcgacgg
    12841 gcgttgcacc ggcaggcagc tcgacaatgc gcccttccgg tgtgaaaacg taaatctcat
    12901 ccgggaagag atcggattta acgctctcga taaattcaaa cgaactaccg gcgctctgtt
    12961 gcagctccag caggctttgc atccagcgct gggcgcgtat ttgtgcggta gtactggttt
    13021 cgccgtgctc tttataagcc cagtgcgcgg caacacccat ctccgccatc tgatccatat
    13081 cttcggtacg gatctggacc tcaaccggca cgccgtgcgg gccaatcatc gaggtgtgca
    13141 acgactgata gccgttcgct tttggaatgg cgatatagtc tttcacgcgg cccggacgcg
    13201 gcttgtacag gctgtgcatc tggcccagca cgcgataaca ggtgtcagaa tcattgacga
    13261 tcacgcggaa agcgtagatg tccatgattg agtgaaaacg ctgctctttg agcaccattt
    13321 tgcagtaaat cgaataaaga tgcttttcgc gaccactgac gcggcacggt attcccgctt
    13381 cctgcaaacg cccttcgatt tcagaaagaa tcttctggat catctcttta cggttgccgc
    13441 gcgcggcttt caccacttct ttgattacgc gataacggtt gggatacagc gcctcaaaac
    13501 ccagctcttc gagttcggtt ttaatgtggt ggatacctaa acggtgcgcc agcgggctgt
    13561 aaatttcgag agtttcacgg gcgatgcggc gacgtttgtc cgggcgaagt gagcccagcg
    13621 tgcgcatgtt gtgggtacgg tcggcaagtt tgatgaggat gacgcggata tcctgcacca
    13681 tcgccataat catcttgcga aagttttcgg cctgcgcctc tttcttatcg cggaacttga
    13741 gtttatcaag tttcgacacc ccctctacca gctcggcgac gcttttacca aaaagctgtt
    13801 ccatatcctg gtaggtggcg ggagtatctt caatcacgtc atgcagcagc gccgccatca
    13861 gcgtttcata gtcgagtttc atctcggcca gaatgcaggc aaccgctacc gggtgcgtga
    13921 tatagggttc accgcttgaa cgtgtttgcc cctcgtgagc atcacgtgca acgagatacg
    13981 cctgccgcag acgcttgatt tggtcttccg gcaggtaggt ttgaatcagt tgattcaggc
    14041 tttcaaacag atacaagggc gacccgcttt gtgattaacg acgaccttca gcaatagcgg
    14101 taacggcttg taattcagcg gcttcctgct cttgctgttc ctggcgttcg cgaacgtcga
    14161 ggatctggtt gttgatcaga ccttcttcga tttcgcgcag cgcgattaca gtggttttat
    14221 cgttttcttc cggtaccagc ggatcctttc cgcctacctg catctgacga gcgcgacgcg
    14281 cggcgaccag taccaggtca aaacggttac caattttctc tacagcgtcc tgaacagtta
    14341 cgcgtgccat acttaaaaag ctccacaggt gaagaaatga ctgggcatga tactgaaatc
    14401 aggttcagtc tgccaacaat ttgctgatta aagcgtcatg acgctgcttt tggcggctca
    14461 tgcgcagacg ttcggcgcga ataatggtct tcaaatcggt caacgcggta tcgaagtcat
    14521 cattcacaat caggtaatca tattcgtcgt aatggctcat ttctgcaaca gcttgcgcca
    14581 tacgctttgc aatgacctct tcgctgtcct gaccgcgacc gcgtagacgg cggtccagtt
    14641 caattttaga cggcggtaaa ataaagatac tccgcgcgtg cggcatcttc tggcgaattt
    14701 gctgcgcgcc ctgccagtcg atatcgagaa aaacatcgac accggtcgcc agtacttgct
    14761 caatggcctc acgcgaagtg ccatagtaat taccaaaaac ttctgcgtgt tcgaggaacg
    14821 catctctgct aatcatttct ttaaattcat catgattaac aaagaaataa tgttcaccgt
    14881 ggacttcacc aggacgcggt tggcgtgtgg tgtgtgaaac agaaacctgg gtgtcataca
    14941 acggttgggt ttttaataaa gcctgaatca ggctggattt acccgcgcca ctgggggcag
    15001 aaacaatata aagcgtgcct tgagccatga gtatctttcg tatgtgatta gcgaaataaa
    15061 gcctacatac gagcttatta tacacggcgc ggccatgtga cgtagccctt gctcacactt
    15121 tttgcgcaaa gttgttgcgt tttgcgtagc tgcttccgca tttgcctgtg atttactgca
    15181 ataaagacga ttctcaggaa gcgtcctcgc agactccgtt tttgccactc ggctttgcct
    15241 tcaccacgtt gtatatctgc cagcctgact acagcggagg tgaagatgaa agtatggatg
    15301 gcgatattaa taagtatctt gtgctggcaa tcatctgtgt gggcggtctg tccggcctgg
    15361 tcgccagcca gagcacagga agaaattttc cgccagcaac agcaaataaa acagtgggac
    15421 gatgactact ggaaggaagg aaaaagtgag gtggaagacg gtgtttacga tcagttaagc
    15481 gcccgtctta cgcagtggca acgctgcttt gtgagcgagc cccgcgacgt gatgatgccg
    15541 cctttaaatg gtgcggttat gcatccggtt gcccatacgg gtgtacgcaa aatggcggat
    15601 aaaaacgcat taagtctgtg gatgcgagaa cgtagcgatc tttgggtgca gccaaaagtt
    15661 gatggcgttg cggtaaccct ggtttatcgg gacgggaaac tgaacaaagc aatcagtcgc
    15721 ggtaatggcc tgaaaggcga ggactggacg cagaaagtta gcttaatttc cgctgtgccg
    15781 caaaccgtta gcgggccttt agccaacagt acgcttcagg gggaaatatt tctccagcgc
    15841 gaggggcata tccaacaaca aatgggggga ataaatgccc gcgcaaaagt tgctggcttg
    15901 atgatgcgcc aggacgatag cgacacgctg aattctctgg gcgtttttgt ctgggcatgg
    15961 ccggatggac cgcagttaat gtctgatcgt ttaaaagagc tggctaccgc aggttttact
    16021 ctaacgcaga cgtatacccg tgcggtgaaa aatgctgatg aagttgcgcg cgtacgcaat
    16081 gagtggtgga aagcggaatt acccttcgtc accgatggcg tagttgtacg agcggcgaaa
    16141 gagccagaat cccgccattg gctaccgggc caggcagagt ggctggtggc ctggaaatat
    16201 caacctgtag ctcaggttgc cgaagtgaag gcgattcagt ttgcggtggg taagagcggt
    16261 aaaatatcgg tggttgcgtc actcgcatct gtcatgctgg atgataaaaa agtccagcgg
    16321 gtgaatattg gttccgtcag gcgctggcag gagtgggata ttgcgcctgg tgatcagatt
    16381 ctcgtcagcc ttgccggtca gggtattcct cgcattgatg atgtggtgtg gcgcggtgca
    16441 gaacgtacaa aaccgacacc gccagaaaac cgctttaact cgttgacctg ctactttgct
    16501 tctgatgttt gtcaggaaca gttcatttca cgcttagtct
//
LOCUS       DAZPEB010000083        16525 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZPEB010000083 DAZPEB010000000
VERSION     DAZPEB010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16525
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.084"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            97..2325
                     /gene="crfC"
                     /locus_tag="ACHURJ_002297"
     CDS             97..2325
                     /gene="crfC"
                     /locus_tag="ACHURJ_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFR1281227.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAAQLAL
                     PWSQPNIAERYAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEIRTCCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHVNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            2322..3205
                     /locus_tag="ACHURJ_002298"
                     /pseudo
     CDS             2322..3205
                     /locus_tag="ACHURJ_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
     gene            3469..4971
                     /gene="proP"
                     /locus_tag="ACHURJ_002299"
     CDS             3469..4971
                     /gene="proP"
                     /locus_tag="ACHURJ_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFR1281228.1"
                     /translation="MLKRKKVKPITLRDVTIINDSKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            5037..5174
                     /gene="pmrR"
                     /locus_tag="ACHURJ_002300"
     CDS             5037..5174
                     /gene="pmrR"
                     /locus_tag="ACHURJ_002300"
                     /inference="COORDINATES: protein motif:HMM:NF033224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFR1281229.1"
                     /translation="MSDAHTLLQVNGGECNEKPCLYESLTTVFSVLVVSSFLYIWFAT
                     F"
     gene            complement(5192..6250)
                     /gene="pmrB"
                     /locus_tag="ACHURJ_002301"
     CDS             complement(5192..6250)
                     /gene="pmrB"
                     /locus_tag="ACHURJ_002301"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFR1281230.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRA"
     gene            complement(6251..6919)
                     /gene="basR"
                     /locus_tag="ACHURJ_002302"
     CDS             complement(6251..6919)
                     /gene="basR"
                     /locus_tag="ACHURJ_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFR1281231.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKSRIRTVRGFGYMLVA
                     NEEN"
     gene            complement(6916..8559)
                     /gene="eptA"
                     /locus_tag="ACHURJ_002303"
     CDS             complement(6916..8559)
                     /gene="eptA"
                     /locus_tag="ACHURJ_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFR1281232.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTV
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            complement(8663..10000)
                     /gene="adiC"
                     /locus_tag="ACHURJ_002304"
     CDS             complement(8663..10000)
                     /gene="adiC"
                     /locus_tag="ACHURJ_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFR1281233.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTPGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            complement(10137..10898)
                     /gene="adiY"
                     /locus_tag="ACHURJ_002305"
     CDS             complement(10137..10898)
                     /gene="adiY"
                     /locus_tag="ACHURJ_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFR1281234.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            complement(11223..13490)
                     /gene="adiA"
                     /locus_tag="ACHURJ_002306"
     CDS             complement(11223..13490)
                     /gene="adiA"
                     /locus_tag="ACHURJ_002306"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFR1281235.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTTDFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSPHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     YWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHAMCVKA"
     gene            complement(13689..14597)
                     /gene="melR"
                     /locus_tag="ACHURJ_002307"
     CDS             complement(13689..14597)
                     /gene="melR"
                     /locus_tag="ACHURJ_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFR1281236.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMMIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            14880..16235
                     /gene="melA"
                     /locus_tag="ACHURJ_002308"
     CDS             14880..16235
                     /gene="melA"
                     /locus_tag="ACHURJ_002308"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFR1281237.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATLLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPSTLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPHIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGESSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            16338..>16525
                     /locus_tag="ACHURJ_002309"
     CDS             16338..>16525
                     /locus_tag="ACHURJ_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1281238.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMG"
BASE COUNT         3988 a         4001 c         4351 g         4185 t
ORIGIN      
        1 attatttctt gcagtaatta taaaagccga aaacagaact caaaaacaat ctggctaaat
       61 aaaagtaaca aaatttgctt taaggaagaa ttttctatgt acacacagac cctgtatgag
      121 ttaagtcagg aggctgaacg cctgttacag ctttctcgcc aacagttgca gttactggaa
      181 aaaatgcctc tctctgtacc cggagacgat gcggcacaac tggctttacc ctggagtcag
      241 cctaatatcg ccgaacgtta cgcgatgctg aataatgagt tgcgtaaaat ttcccgactg
      301 gaaatggtgc tggcgattgt cggtactatg aaagcaggga aatcaaccac cattaatgcc
      361 attgttggta cggaggttct gcctaatcgt aatcgcccaa tgactgcgct gccgacgctt
      421 attcgccata cgcccgggca aaaggaaccg gtactgcatt tttcacatgt cgcgccaatc
      481 gattgtttaa ttcaacaatt acaacagcgc ctgcgtgatt gcgatattaa gcatctgacc
      541 gatgtgctgg aaatagataa agatatgcgt gcgcttatgc agcggatcga aaatggcgtc
      601 gctttcgaaa aatattatct gggtgcccag cctatttttc attgtctgaa aagtttgaat
      661 gatttagtgc gactggcgaa ggcgctggac gtcgattttc ctttttctgc ttacgccgcc
      721 attgagcata ttcccgtgat tgaagtggag tttgtccatc tggcggggct ggaaagttat
      781 cccggtcagt tgacgttact ggataccccc gggccaaatg aagccgggca accgcatctg
      841 caaaaaatgc ttaaccagca gctggcacgc gcctcggcgg tactggcggt gctggattat
      901 acgcaactga aatcgatctc cgatgaagag gtccgtgagg cgattttggc ggtggggcaa
      961 tcggtgccgc tgtatgtgct ggtcaataag ttcgatcaac aggatcgtaa cagtgacgac
     1021 gccgaccagg tgcgggcact gatttccggg acgctgatga aaggctgtat tacgccacag
     1081 cagatatttc cggtgtcgtc gatgtggggc tacctggcga atcgggcgcg ctatgagtta
     1141 gccaacaacg gtaagttacc agcgccagag caacaacgct gggtggaaga ttttgcccat
     1201 gccgcgctcg gcaggcgctg gcgtcatgcc gatctggcgg acctcgaaca tattcgtcat
     1261 gctgccgatc agttgtggga agattcgctg ttcgcccagc caattcaggc gttgcttcat
     1321 gccgcttacg ctaacgcctc attgtatgct ctgcgatctg ctgcgcataa actgttgaat
     1381 tacgcgcagc aggcgcggga atacctggat tttcgtgcgc acgggttaaa cgtcgcttgt
     1441 gaacaattgc ggcaaaatat ccatcaggtc gaagaaagtt tgcagctatt gcaactcaat
     1501 caggcgcagg tgagcggcga gattaaacat gaaatcgagc tggccctgac ctccgccaac
     1561 cactttctgc gtcaacagca agatgcgctg aatgcgcagt tagccgcctt gtttcaggat
     1621 gattcggagc cgttaagcga gattcgtacc tgctgtgaga cactgttaca gacggcgcag
     1681 aacaccatca gtcgcgactt tacgctgcgt tttgccgagc ttgaatccac cctttgccgg
     1741 gtgttaaccg atgttattcg gcccattgag caacaagtca aaatggaatt gagcgagtca
     1801 gggtttcgtc ctgggtttca ttttcctgtt tttcacggag tagttcccca cgtcaacact
     1861 cgccagctgt tcagtgaagt catttcgcgc caggaggcaa cggacgagca gagcacgcgt
     1921 ttaggcgttg tgcgtgagac tttttcgcgc tggttgaatc agcccgactg gggacgggga
     1981 aatgagaaat ccccgacaga aacggttgat tacagtgtgt tgcaacgagc attaagcgca
     2041 gaagtcgatc tttattgcca acaaatggct aaagttctgg cagagcaggt cgatgaatct
     2101 gttacggcag gcatgaatac ttttttcgct gagttcgctt catgtttgac ggaattacag
     2161 acgcgtttac gcgaaagcct ggctctgcgt caacaaaatg aatcggtggt caggctgatg
     2221 cagcagcaat tgcagcagac tgtgatgact cacggctgga tttacaccga cgcccagctg
     2281 ttacgcgatg atattcaaac acttttcacg gcagaacgat attgaccaag acgttactgg
     2341 acggccccgg tcgcgtgctg gagtcggttt atccccgctt tttagtggat ctggcgcagg
     2401 gcgatgatgc ccgccttcca caagcccatc agcagcagtt tcgtgaacga ctgatgcagg
     2461 aacttctttc gcgtgtgcag cttcagacat ggacgaacgg cggcatgtta aatgcgccgc
     2521 ttagcctgcg actgacaatg gtggaaaaac tggcgtcgat gctggatccc ggtcatctgg
     2581 cgctgacgca gatcgcgcag catctggcgc tgctgcaaaa aatggatcac cgccagcact
     2641 ctgctttccc ggagctcccc cagcaaattg ccgccttgta tgagtggttt tcagcccgtt
     2701 gtcgctggaa ggaaaaggcg ttaacgcaac gaggccaggc ctactggtgc aggcaggtga
     2761 tcagagcgag caaattttta cccgctggcg tgctggggcg tataacgcct ggtcgttgcc
     2821 tgggcgctgt tttatcgttc tcgaagagtt gcgttggggg gcatttggcg atgcctgtcg
     2881 tctgggaagc ccgcaagcgg tggcgttgct gctgggtgat ttgcgcgtga aagcgacaca
     2941 acatctggca gagagtatca atgcggcccc gaccacgcgt cactattacc atcagtggtt
     3001 tgcctcttcg accgttccga cgggcgggga tcatgctgat tttttaagtt ggctgggaaa
     3061 gtggaccacg gcagataaac aacccgtttg ctggtcagtg acccaacgtt ggcaaactgt
     3121 cgcgctgggg atgccacgac tctgttcagc gcagcgtttg gtgggggcaa tggtcgagga
     3181 aatcttctct gtaaatttgg cgtaaataat cagttacatc aatgagtcct aaacgaaatc
     3241 catgtgtgaa gttgatcaca aatttaaata ctggtagggt aaaaaggtca ttaactgccc
     3301 aattcaggcg tcaactggtt tgattgtaca ttccttaacc ggagggtgta agcaaacccg
     3361 ctacgcttgt tacagagatt gcatcctgca attcccgctc cccttttgcg gccgtcgcgc
     3421 tgatttttct ggcgtttgcg gaaatgggcc aactctgcga ggaaagctat gctgaaaagg
     3481 aaaaaagtaa aaccgattac ccttcgtgat gtcaccatta ttaatgacag taaactgcgt
     3541 aaagccatta ccgcagcatc actgggtaat gcaatggaat ggttcgattt tggtgtttat
     3601 ggttttgttg cttacgcatt aggtaaagtt tttttcccgg gggctgaccc cagcgtgcag
     3661 atggttgctg cacttgccac tttctccgtt ccctttctga ttcgaccgct tggcggactc
     3721 ttctttggta tgttgggcga taaatatggt cgccagaaga tcctcgctat cactattgtg
     3781 attatgtcga tcagtacgtt ctgtattggc ttaataccgt cctacgacac gattggtatt
     3841 tgggcaccga ttctgctgtt gatctgtaag atggcacaag gtttctcggt cggcggtgaa
     3901 tataccgggg cgtcgatatt tgttgcggaa tactcccctg accgtaaacg tggctttatg
     3961 ggcagctggc tggacttcgg ttctattgcc gggtttgtgc tgggtgcggg cgtggtggtg
     4021 ttaatttcga ccattgtcgg cgaagcgaac ttcctcgact ggggctggcg tattccgttc
     4081 tttattgctc tgccgttagg gattatcggg ctttacctgc gccatgcgct ggaagaaact
     4141 ccggcgttcc agcagcatgt tgataaactg gaacagggcg accgcgaagg tttgcaggat
     4201 ggcccgaaag tctcgtttaa agagattgcc actaaatact ggcgcagcct gttgacatgt
     4261 attggtctgg taattgccac caacgtgact tactacatgt tgctgaccta tatgccgagt
     4321 tatttgtcgc ataacctgca ttactccgaa gaccacgggg tgctgattat tatcgccatt
     4381 atgatcggta tgctgtttgt ccagccggtg atgggcttgc tgagtgaccg ttttggccgt
     4441 cgtccgtttg tgctacttgg tagtgttgcc ctgtttgtgt tggcgatccc ggcgtttatt
     4501 ctgattaaca gtaacgtcat cggcctgatt tttgccgggt tactgatgct ggcggtgatc
     4561 cttaactgct ttacgggcgt tatggcttct accttgccag cgatgttccc gacgcatatc
     4621 cgttacagcg cgctggcggc ggcatttaat atttcggtgc tggttgccgg tctgacgcca
     4681 acactggcgg cctggctggt cgaaagctcg cagaatctga tgatgcctgc ctattacctg
     4741 atggtagtgg cggtgattgg tttaatcacc ggcgtaacca tgaaagagac ggcaaatcgt
     4801 ccgttgaaag gtgcgacacc ggcggcgtca gatatacagg aagcgaagga aattctcgtc
     4861 gagcattacg ataatatcga gcagaaaatc gataatattg accacgagat tgccgatttg
     4921 caggcgaaac gtacccgcct ggtgcagcaa catccgcgaa ttgatgaata agctgaaacg
     4981 gatggcctga tgtgacgctg tcttatcagg ccaattgaac tcttaaggtt cacttaatct
     5041 ctgacgcgca tactctcctc caggttaacg gaggagagtg caatgaaaaa ccgtgtttat
     5101 atgaaagttt aactaccgtg ttcagcgtgc tggtggtcag cagctttctt tatatctggt
     5161 ttgccacgtt ctgatctttc ttcagccgta cctaggcccg cgtgccggaa gtctcttgcc
     5221 ggttttgcag gaaaaactgc ccgtgatgca actgtgtaat gcggctgaca atacttaacc
     5281 ccagaccaat cccgccataa cggctgtcca tacgtacaaa cgctttactc aactccccgc
     5341 atttactctc atcaatacct ggtccttcat cttcaactgc catgaccgct ccgccatctt
     5401 cttgcagctt aatcataatg ttgctgcctt gcgggctgta acgatgggcg ttttctacca
     5461 ggtttcgcaa taacatccgc agcagggttg catcaccctg aacggtgatg tcggcggcgc
     5521 tctctggcaa tagcagggtt tgctgtcgct ggtcgagcat ggtactgagt tcgtcatacg
     5581 aggggagaat gacatcttcc agcagtttta catgttgata attaccggaa gaaaatgact
     5641 gtccggcacg cgccagttgc agcagctggg agacgctctc catcatctga tcaagccgtg
     5701 ccactaacgg tgctacatca atgtgatgcg ttttcgccag cagttccaga tgcaaacgca
     5761 cccccgccag tggcgttcgc agttcgtgcg cgacgtcagc ggtaaacaac ctttcgttat
     5821 ccagcgtgct ggtcaggcga ctgaccagat cgttcaacgc cgaaaccacc gcttcgattt
     5881 cgagggtggc gctgtgaatg gcaatgggcg ttaagttgtc ggcagtgcgc gcttccagct
     5941 ctttttgcag ctccgccagc gggcgggtga tgcggcgtac cgcctgatag cagataaata
     6001 gcgtcaggct gaccataaag acgccgggga caatcaggct ggcgaccgcc tcgcggatct
     6061 cacgcatgat gtggcgatcg ttgttgcgat tgtcgcgtag cgcctgctca aacagctgaa
     6121 tctgctcggt actttcatgc catagccaga agacgctgat cagctcaaac accaacaaaa
     6181 tggccccgat agtcaatatc agccgttggc gcagcgatat tggtcggcgc agaaaacgca
     6241 tcagattcaa ttagttttcc tcattcgcga ccagcatata gccaaagccg cgcacggtgc
     6301 ggatacggga tttgcccact ttgtcgcgca gattgtggat atgcacttcc agggtgttgg
     6361 tcgagggttc attgtcccag ttatagatgt cgttgtagag aatttcccga tgtaccggac
     6421 tgcctgcttt gagcattaac cgtgacagca gagcatattc tttgggcgtc agaatcaact
     6481 cttcaccgcc catccatacc tgacggcgac ccatattcag cgtcagattg ccaacaatca
     6541 gctcactttc gccctgatta ttatggcgtc gtagcagggc gcggatacgg gcgtgtaact
     6601 cttccagcgc aaaaggcttc accagatagt catcggcacc gacatccagc ccggcgattt
     6661 tgtcggtcag cgtatcgcga gcggtgagga tcagtaccgg cagggtatat tttttctgcc
     6721 ggatacgggc gagaaaatgc agtccatctt cgtcgggtaa ccctaaatcc agtaccacca
     6781 ggctgtaatg accggcttca aggctttgtt ccgccatccg cgcggttgtc acgccatcgc
     6841 acgcgtagcc ttcggtttgc gccgccagaa tcagtccctg caataacagc gtatcgtctt
     6901 caacaatcag aattttcatt tactcactct cctgcaagtt tgcagaatat catccgcagc
     6961 ctggtaatac ttcgtctcaa cgccagttaa tcccaatagc gtggagaata aattgtcttg
     7021 tgaatagtgt tgcgtttgcg cctgtttttg caggcagttc tggtcaacct gataccgttt
     7081 ttgataatcc tccgacagcc acagcagcat cggcacctgt ttttggctat ccggggcgat
     7141 ggcataaggc agaccgtgca gatagatgcc attttcacct aacgattcac cgtggtcaga
     7201 aagataaacc aggctggtgg taaatttatc ctgatgttct ttcagcagat taatcgcttt
     7261 atcaacaata tagtcgacgt aaaccagcgt gttgtcgtaa gtgttcacca gttgctcttt
     7321 ggtacaggtc tggatctcat tggtgtcgca ggttggggta aatttcctga actgcggcgg
     7381 atagcggttg taataggtcg gaccgtggct gccgatggtg tgtaagacaa tcacgccatc
     7441 accctgcagg ttattgatgt actcttccag cccgtggaac agcacttcgt catagcattc
     7501 gccgttgatg cactgaccag gcagattcag cgcggtgacg ttctggtgag gcacgcggtc
     7561 gcaggcacct ttacagccgc catcgttgtc attccacagc acgttgatgc ccgctcgctg
     7621 aatgatatcc agcacgcctt cctggtgctg tgccagctct tctttgtaat gctcacgcgg
     7681 catatccgag aacatgcacg gtactgaaac tgccgttgcc gtgccgcaag atacggtatt
     7741 agggaaatag accacgttat ctttcgccag ccgcgggtta gtttcacgcg ggtagccgtt
     7801 gagggagaag ttctccgccc gcgaggtttc gccgacaatc aggatggtca aatttttacg
     7861 tttttcgttc tgcattaacg ggttgcggtg ggcgtcttca ccaattcgca ccagcggtag
     7921 atttgccagt cgctgatggg agtaccatga ccagctggca acaatgctgt tagaggggct
     7981 taaggatttc accagctctt tgttattgcg gaacaacgag gcgtagtctt tataaaacag
     8041 tgcggcgacc agcaaaatca gtagtacaga aaccagaata ttggctccac ggaaaagaac
     8101 actgcgcaga cgcgaggtgg caggtttgat ttttatccag caggcaatca gcgcagcaag
     8161 cacgccgctg aatcccagcg ttaataacat ttgcggtgtc atcagcgcat aactttctgc
     8221 cggagtggta tcaataatat tggcaatcat cgagcggtcg atgacgatgc cgtaagtcat
     8281 tatgaaatat tgtgcagccg cgccaaccag aataaacagg caggccagtg gtcgattaag
     8341 ccataagaaa gagcttagtg tcaggacaat attaatcacg ctgaaagcga cgaccggcat
     8401 cgacaagaaa accagtacgt tatgcagcga atccagcggc agcgcctgca acacctgttt
     8461 aaaaaaggca atattcaggc agatagagat ataaaaagcg gccaacaata gccaggcgag
     8521 taaattcaaa gagggtcttt ttagtaggcg cttcaacatc acggtgtttc catcgaacaa
     8581 agtgcgcata tgctcgcaaa gcaaaattaa gccaacctta agttcttaag gttggctttt
     8641 atgtttgctg gattaagggg aattaatctt tgcttattgg tgcatctaag ggatacgggt
     8701 ttttatgtag ccggttgtaa ttcagcgcat acatggcggt gatgaccatc agggtgacaa
     8761 atgaccacat aacctctttc gctccggacc ccaccacggc ccagatgcag tagaggaagg
     8821 caatggtagt aactgccaga tatgccgggc gtgctttacc aaagtgaccg tgtccgagca
     8881 gcagtaacgc cgcacaggtg taaagatatg gcaccagtgt aaagatgacc gagacggaag
     8941 aaaccagacc gaactctttg gtcgcgtttg gtgaaatgct gctgagctgg aagatggtca
     9001 tcaaaatacc gacgataatc agccccgcca ctggcgtacc cgctttattt acacgggcaa
     9061 aaatcggtgg gaacagtccg tcatcggcag cggctttcgc cgtttgaccc gccagcaacg
     9121 tccagccgcc cagtgaacct aagcaacccg cagctgcgca gaaggaaaca atggccccgg
     9181 gggtgtcacc caacgccatc cgtgcggcat caccgaatgg cgaagcagaa acgcgcagtg
     9241 cggcattagg gatcatcccc ataatcgcgg tggtagaaag tacatagcaa acggcggcaa
     9301 tcaatacccc accaatggtg gcgataggga cattgcgttt cgggtttttc accacacctg
     9361 cggcaacgga ggcactttcc acaccgatga acgaccacag cgtaacgtta agggtacttt
     9421 gaattgcacc gaaggtaccc aggccgctga cgttccatgc cgccatatag gtttcaccac
     9481 ggaaccagaa ccagccaaat acggcaatcc cgacgatggg gatcagcgcc agcacggtgg
     9541 caactgcctg cacacgggtg atcattttcg gaccaacaat gttcagcagg acgaagatcc
     9601 acagcaccac gacgcaggtg atggttaata ccaatggatc tttcagaatc gggaagaagt
     9661 aacttaaata tcctacgcca atgaccacca tggcgatatt gccgatccag caggccagcc
     9721 agtagaggac gttggtttga taaccgagaa acgggccaaa gcagcggcgg gcgtaagcgt
     9781 aagaaccacc aggacttggg tcgaggaacg acattttggc gtataccatc gagagcccca
     9841 gtgcaccgat aatcgtcacc aaccatccat aaatggcaat cccgccagtg gaggccaggt
     9901 ttgcaggtaa cagaaaaaca cctgacccca taatattccc cgacaccatc agggtgacgg
     9961 ggattaagcc cactttgtga gcatcagcat ccgaagacat aattaaactc ctgcgaaggc
    10021 gagcttcgtg acaataaatt acgtcatatc atacgcctgc attcgatagt tgaattatta
    10081 tcgaccgggt tatcgctgat gccgttatta atatagctct tcgttaaggt taaaaatcag
    10141 gcgacagtgc gttctctgtg ctgactaaca taatgcagcg gcgtcatacc gtagaagtct
    10201 ttaaagacag aaataaagta cgacgtactg ttgtagccgc aggactgtga tacctgagag
    10261 atatttttac cgtccatcat taattcattt acggcataac gcatgcggca ggtggtgatt
    10321 atttggctat aactggtgtt ttcgcttttc agctttttct ttaacagact tgggctaaga
    10381 cataaacaac tggctaccat actaagattc cagtctttgt gaatatcgct ttcaataatt
    10441 tgataaacgc tgtcacttac acaattacgt aacatataca tcattaatga aaggaatttt
    10501 ttactgtcga gaaaacggga tagcaccgtg aataataatg cgcgtgttcg ttccttttct
    10561 gactcagttt ctgcgggcat catgctgtgt tgcgcggcaa cgcgaaagac gtctggcgtc
    10621 aggcatggca gggtgagtat gggcgtagcg ctacgttgcc atactggtat ttgcgagaga
    10681 tctttattca gaaaccggag gtaatctttg atgatgtcgt gactaatatg agcaaccaaa
    10741 gtgttgttaa gagaggagac gtcgataata ttattttcac aatttaataa cgccatatgg
    10801 ttagctttaa ggctaatagg ttctttccca ttcaccctta tccagacatc tttttcagtc
    10861 aataaaacaa tacaaggttg gtcgctgcaa atcctcatac atgtactcct gagtgcgaat
    10921 aaaagaccag ttctcgctaa agcaaagcga taccgggaaa tggttatata attgtatgac
    10981 gaatataaac atgcataaaa ataacgagtt ttactaatat tacaaatttt tatagtaatt
    11041 tctcatgtac atattttttt acagggtaaa taacaggcaa aaaaaagcgc ggggtggggc
    11101 cgcgcaaaaa gtatgacatt tttgtttgca gcaattttta tgaaaacgtt gccatactca
    11161 aaaggcctaa ggccaggcgg cgtaatgtta tttaaacaat tacgccttca gcggaatagt
    11221 ggttacgctt tcacacacat agcgtggtaa ataccgtcaa taatttcagt cccttcagtt
    11281 tcgtgttcaa atccagggaa atggtggtcc caggattgca gcgagcgtaa ataacttact
    11341 tgcggactgt ttttatcgcc gaagttttca ccagacagca gcatcgggat tcctggcgga
    11401 tatgggataa ctgagtttgc cgcgatgcgt cctggcagat tttcaatgga taccagttcg
    11461 acattgttgt cgacaatcgc gttgtacgct tcacgcgggg tgatttccgc caccggcagg
    11521 ccggaatagg cttcgttcaa ccgtgcgcca gggttgtttt ctttcagcca ggcaaacatg
    11581 gtgtcaccca gatcgtgaat ccccatgttc gcgtaagtgt caggatattg ttcaacaagt
    11641 tccggcatca cctgcgccag cggtgtgttg gcgtcatagt ggcgtttgaa ggagcaaagg
    11701 gtgttaacca gagtccccca tttcccacgg gttacgccca tagagaacag gaacataatt
    11761 tggaagtcag tggtgcgggt aggcacaatg ccgtggcgac caagccaggc agtgaccagc
    11821 gccgccggaa caccggtttc ttccagttca ccatcttcac ccattcccgg agcaaggatg
    11881 ctgactttaa tcgggtcgag catactccag ttatccggaa tatctttgaa gccgtgccag
    11941 ctttcgcccg gatgcattac ccagtagtcc tgaacggtgg tcagcagttt ggttggtgcg
    12001 tcagcaaagt cataggtttt gccggtttgt gggtcggtga cgacttcttt gttccacggt
    12061 ttgaagaacc agctaccgtc agcggtgaac tctttataca gccgcgccat cgcctgacgg
    12121 aaatcaaccg cttcgtcgat cacttcctgc gtcagtgaca ggccgctgtt gccgtccatc
    12181 atcgacaccg ccacgtcgtt ggatgcgcag atggcataca gcggggaggt ggtggcatgc
    12241 atcatgtagg cctggttgaa gcgggagaag ttaatcgccc cacgaccttc acgtacatga
    12301 atataagaag cctgtgaaag cgcattcagc agtttgtggg gggagtgggt ggcgaaaacg
    12361 gtaggaccgt tgtgatcgcc aggttcgccg cgcatggcat agtgatcggc atagatcggg
    12421 ttgaaacgtg catagccgta ccaggcttcg tcaaagtgca gacgatcgga ggttttttcc
    12481 agcagatcct gcgcttcttt agcgttataa cacacgccgt cataggtgca gttggtcacc
    12541 acgcagtaag acggtttttg cccggctttg tctttagtca gcgggctttc actgattttc
    12601 ttctgcaagg tttcaggttg catttcctgc ggatagattg gcccgataat gccgtagcgg
    12661 ttgcggcttg gcaccatgta gaccggtttc gcgcctgtca gcatcaagcc ttgttcgatg
    12721 gatttatggc agttacggtc aacgaccacg acatcgttat cggtcatgca agcctgcatg
    12781 atggtgcggt tagagccgga agtaccgacg actaccgacc aggagcgatc ggcaccaaat
    12841 acgcgtgcgg catatttttc gctttcgcca aatgcgccag tatggtcaag caaagaaccg
    12901 agggaagttc gttcgatgcc catatcggtg cggaacagat tttctccata gtagtcatgg
    12961 tagaaacgtc cggcgggtgt tttggtaaaa ccaacgccgc cctggtggcc tggcgctgcc
    13021 caggaatatt catggatgtc gctatacttc atcagcgcgc tgaacagtgg cggcaacagc
    13081 tgctggcggt agcgggtcat cgcggcaacg gcgcgtccgg cgataaagtc ggtggtatct
    13141 tccagaatcc aggcgaattc atcgacaagc tccagcaggt cgcgatccat tgcggcgagg
    13201 gctttttccc gatcgcccaa caggaagacc ggcacgtttt gttggcgctc atgaagctta
    13261 ccgatcaatt gtctgacgtt ttgatgttcg tccggatgtt ccatttgata gctgaacatc
    13321 aggcagtcaa tggcttcgtt tgaagagaga atggcaaaac catcatcaaa ggaggtggat
    13381 ttaatcacgg taacattttg ctggcttaaa gcatctgcca gacgctcaac ggcgttaccg
    13441 acccaggtgt cttgatggag aaactcgctt tcaacaatta atactttcat cattgcttac
    13501 ccggttatga aggaatcttc gttgcgggca agtatcttcc ggccttttcg ggctgtaaag
    13561 cgcgtgaaaa acaaacattt ttgaaatatt tatttttata aacaatgcgt tatgtgatta
    13621 ttttaaatta ttttcactgc ttgttatcga cggctaaact atttttttgg ctgatactga
    13681 tatcgtcttt agccgggaaa cgtctggcgg cgctgttggc taagtttgcg gtattgttgc
    13741 ggcgacatgc cgacatattt gccgaacgtg ctgtaaaaac gactacttga acgaaagcct
    13801 gccgtcaggg caatatcgag aatactttta tcggtatcgc tcagtaacgc gcgaacgtgg
    13861 ttgatgcgca tcgcggtaat gtactgtttc atcgtcaatt gcatgacccg ctggaatatc
    13921 cccattgcat agttggcgtt aagtttgacg tgctcagcca catcgttgat ggtcagcgcc
    13981 tgatcatagt tttcggcaat aaagcccagc atctggctaa cataaaattg cgcatggcgc
    14041 gagacgctgt ttttgtgtgt gcgcgaggtt ttattgacca gaatcggttc ccagccagag
    14101 aggctaaatc gcttgagcat caggccaatt tcatcaatgg cgagctggcg aatttgctcg
    14161 ttcggactgt ttaattcctg ctgccagcgg cgcacttcaa acgggctaag ttgctgtgtg
    14221 gccagtgatt tgatcatcat gccgtgagtg acgtggttaa tcaggtcttt atccagcggc
    14281 caggagagaa acagatgcat cggcagatta aaaatcgcca tgctctgaca ggttccggta
    14341 tctgttagtt ggtgcggtgt acaggcccag aacagcgtga tatgaccctg attgatattc
    14401 actttttcat tgttaatcag gtattccaca tcgccatcga aaggcacatt cacttcgacc
    14461 tgaccatgcc agtggctggt gggcatgata tgcggtgcgc gaaactcaat ctccatccgc
    14521 tggtattccg aatacagcga cagcgggctg cgggtctgtt tttcgtcgct gctgcacata
    14581 aacgtatctg tattcatgga tggctctctt tcctggaata tcagaattat ggcaggagtg
    14641 agggaggatg actgcgagtg ggagcacggt tttcaccctc ttcccagaga ggcgagggga
    14701 gcgtccgagc atcatgaggc cgaaaactct gcttttcagg taacttatta ccataaactc
    14761 agatttactg ctgcttcacg caggatctga gtttatggga atgctcaacc tggaagccgg
    14821 aggttttctg cagattcgcc tgccatgatg aagttattca agcaagccag gagatctgca
    14881 tgatgactgc acccaaaatt acatttatcg gcgctggttc gacgattttc gttaaaaata
    14941 ttcttggtga tgtgttccat cgagaggcgc tgaaaacggc gcatattgcc ctgatggaca
    15001 ttgatcccac ccgcctggaa gagtcgcata ttgtggtgcg taagctgatg gattcagcag
    15061 gggccagcgg caaaatcacc tgccacaccc aacagaaaga agccttacag gatgccgatt
    15121 ttgtcgtggt ggcatttcag attggcggtt atgaaccttg cacggtgact gatttcgagg
    15181 tctgtaagcg gcatggtctg gaacaaacca ttgccgatac gttggggccg ggcggtatta
    15241 tgcgcgcgct acgtaccatt ccgcatctgt ggcaaatttg cgaggacatg acggaagtct
    15301 gccccgatgc cacattgctc aactacgtta acccaatggc gatgaatacc tgggcgatgt
    15361 atgcccgcta tccgcatatc aaacaggtag ggctgtgcca ttcggtgcag ggaacggcgg
    15421 aagagctggc gcgtgacctc aatatcgatc cgtccacgct gcgttaccgt tgcgctggta
    15481 tcaaccatat ggcgttttac ctggaactgg agcgtaaaac cgccgacggc agttacgtga
    15541 atctctaccc ggaactgctg gcggcttatg aagcagggca ggcaccgaag ccacatattc
    15601 atggtaatac tcgctgccag aatattgtgc gctacgaaat gttcaaaaag ctgggctact
    15661 tcgtcacgga atcgtcagaa cattttgccg aatatacgcc gtggtttatt aagccaggtc
    15721 gtgaggattt gattgagcgt tataaagtac cgctggatga gtacccgaaa cgctgcgtcg
    15781 agcagctggc gaactggcat aaagagctgg aggagtataa aaacgcctcc cggattgata
    15841 ttaaaccgtc acgggaatat gccagcacaa tcatgaacgc tatctggact ggcgagtcga
    15901 gtgtgattta cggcaacgtc cgtaacgatg gtttgattga taacctgcca caaggatgtt
    15961 gcgtggaagt agcctgtctg gttgatgcta atggcattca gccgaccaaa gtcggtacgc
    16021 taccttcgca tctggccgcc ctgatgcaaa ccaacatcaa cgtacagacg ctgctgaccg
    16081 aagctattct tacggaaaat cgcgaccgtg tttaccacgc cgcgatgatg gacccgcata
    16141 ctgccgccgt gctgggcatt gacgaaatat atgctcttgt tgacgacctg attgccgccc
    16201 acggcgactg gctgccaggc tggttgcacc gttaaaacgc gactaaacgc tactgcgccg
    16261 ggggatttat tccggcgcac acctctgacg ataccaataa cagaaggcgg gcgttggtaa
    16321 cagcgacccg ataccctatg agcatttcaa tgactacaaa actcagttat ggatttggag
    16381 cgttcgggaa ggattttgcg atcggcattg tgtatatgta cctcatgtat tactacaccg
    16441 atgtcgttgg gctgtctgtg ggtttggtcg gtactttgtt tctggtggcg aggatctggg
    16501 atgctattaa cgatccgatt atggg
//
LOCUS       DAZPEB010000084        16475 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZPEB010000084 DAZPEB010000000
VERSION     DAZPEB010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16475
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.085"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(118..1401)
                     /gene="gltA"
                     /locus_tag="ACHURJ_002310"
     CDS             complement(118..1401)
                     /gene="gltA"
                     /locus_tag="ACHURJ_002310"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFR1281239.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            complement(1607..1714)
                     /locus_tag="ACHURJ_002311"
     CDS             complement(1607..1714)
                     /locus_tag="ACHURJ_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1281240.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            2095..2499
                     /gene="sdhC"
                     /locus_tag="ACHURJ_002312"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             2095..2499
                     /gene="sdhC"
                     /locus_tag="ACHURJ_002312"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFR1281241.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            2493..2840
                     /gene="sdhD"
                     /locus_tag="ACHURJ_002313"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             2493..2840
                     /gene="sdhD"
                     /locus_tag="ACHURJ_002313"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFR1281242.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            2840..4606
                     /gene="sdhA"
                     /locus_tag="ACHURJ_002314"
     CDS             2840..4606
                     /gene="sdhA"
                     /locus_tag="ACHURJ_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFR1281243.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            4622..5338
                     /gene="sdhB"
                     /locus_tag="ACHURJ_002315"
     CDS             4622..5338
                     /gene="sdhB"
                     /locus_tag="ACHURJ_002315"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFR1281244.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            5639..8440
                     /gene="sucA"
                     /locus_tag="ACHURJ_002316"
     CDS             5639..8440
                     /gene="sucA"
                     /locus_tag="ACHURJ_002316"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFR1281245.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYSDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            8455..9672
                     /gene="odhB"
                     /locus_tag="ACHURJ_002317"
     CDS             8455..9672
                     /gene="odhB"
                     /locus_tag="ACHURJ_002317"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFR1281246.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKSPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLITIK
                     ELLEDPTRLLLDV"
     gene            9948..11114
                     /gene="sucC"
                     /locus_tag="ACHURJ_002318"
     CDS             9948..11114
                     /gene="sucC"
                     /locus_tag="ACHURJ_002318"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFR1281247.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPQLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQLDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKVVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            11114..11983
                     /gene="sucD"
                     /locus_tag="ACHURJ_002319"
     CDS             11114..11983
                     /gene="sucD"
                     /locus_tag="ACHURJ_002319"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFR1281248.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(12087..12809)
                     /gene="mngR"
                     /locus_tag="ACHURJ_002320"
     CDS             complement(12087..12809)
                     /gene="mngR"
                     /locus_tag="ACHURJ_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HFR1281249.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            12978..14894
                     /gene="mngA"
                     /locus_tag="ACHURJ_002321"
     CDS             12978..14894
                     /gene="mngA"
                     /locus_tag="ACHURJ_002321"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFR1281250.1"
                     /translation="MNLTTLTHRDALRLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTMHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVPACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAIFGFMCSFDLGGPVNKAAYAFCLGAMVNGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLVGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPCAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            14912..>16475
                     /locus_tag="ACHURJ_002322"
     CDS             14912..>16475
                     /locus_tag="ACHURJ_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFR1281251.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPTDENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGSLQLV
                     DKDSGVRYDRVLQ"
BASE COUNT         3957 a         4134 c         4485 g         3899 t
ORIGIN      
        1 aatatatgaa tgtccgatag tggattttat ttttacaatt ttttgtgttt aggcatataa
       61 aaattaaccc gccatttgaa cggcgggtta aaatatttac aacttagcaa tcaaccatta
      121 acgcttgata tcgcttttaa agtcgcgttt ttcatatcct gtatacagct gacgcggacg
      181 ggcaatcttc ataccgtcac tgtgcatttc gctccagtgg gcgatccagc caacggtacg
      241 tgccattgcg aaaatgacgg tgaacatgga agacggaata cccatcgctt tcaggatgat
      301 accagagtag aaatcgacgt tcgggtacag tttcttctcg ataaagtacg ggtcgttcag
      361 cgcgatgttt tccagctcca tagccacttc cagcaggtca tctttggtgc ccagctcttt
      421 cagaacttca tggcaggttt cacgcattac ggtggcgcgc gggtcgtaat ttttgtacac
      481 gcggtgaccg aagcccatca ggcggaaaga atcattttta tctttcgcac gacgaacaaa
      541 ttccggaatg tgtttaacgg agctaatttc ttccagcatt ttcagcgccg cttcgttagc
      601 accaccgtgc gcaggtcccc acagtgaagc aatacctgct gcgatacagg caaacgggtt
      661 cgcacccgaa gagccagcgg tacgcacggt ggaggtagag gcgttctgtt catggtcagc
      721 gtgcaggatc agaatacggt ccatagcacg ttccagaatc ggattaactt catacggttc
      781 gcacggcgtg gagaacatca tattcaggaa gttaccggca taggagagat cgttgcgcgg
      841 ataaacaaat ggctgaccaa tggaatactt gtaacacatc gcggccatgg tcggcatttt
      901 cgacagcagg cggaacgcgg caatttcacg atgacgagga ttgttaacat ccagcgagtc
      961 gtgatagaac gccgccagcg cgccggtaat accacacatg actgccattg gatgtgagtc
     1021 gcgacggaaa gcgtggaaca gacgggtaat ctgctcgtgg atcatggtat gacgggtcac
     1081 cgtagtttta aattcgtcat actgttcctg agtcggtttt tcaccattca gcaggatgta
     1141 acaaacttcc aggtagttag aatcggtcgc cagctgatcg atcgggaaac cgcggtgcag
     1201 caaaatacct tcatcaccat caataaaagt aattttagat tcgcaggatg cggttgaagt
     1261 gaagcctggg tcaaaggtga acacaccttt tgaaccgaga gtacggatat caataacatc
     1321 ttgacccagc gtgcctttca gcacatccag ttcaacagct gtgtccccgt tgagggtgag
     1381 ttttgctttt gtatcagcca tttaaggtct ccttagcgcc ttattgcgta agactgccgg
     1441 aacttaaatt tgccttcgca catcaacctg gctttacccg tttgttattt ggctcgccgc
     1501 tctgtgaaag aggggaaaac ctgggtacag agctctgggc gcttgcaggt aaaggatcca
     1561 ttgatgacga ataaatggcg aatcaagtac ttagcaatcc gaattattaa acttgtctac
     1621 cactaataac tgtcccgaat gaattggtca atacttccac actgttacat aagttaatct
     1681 taggtgaaat accgacttca taacttttac gcattatatg cttttcctgg taatgtttgt
     1741 aacaactttg ttgagtgatt gtcaaattag atgattaaaa attaaataaa tgttgttgtc
     1801 gtgacctgga tcactgttca ggataaaacc cgacaaacta tatgtaggtt aattgtaatg
     1861 attttgtgaa cagcctatac tgccgccagg tctccggaac accctgcaat cccgagccac
     1921 ccagcgttgt aacgtgtcgt tttcgcatct ggaagcagtg ttttgcatga cgcgcagtta
     1981 tagaaaggac gctgtctgac ccgcaagcag accggaggaa ggaaatcccg acgtctccag
     2041 gtaacagaaa gttaacctct gtgcccgtag tccccaggga ataataagaa cagcatgtgg
     2101 gcgttattca tgataagaaa tgtgaaaaaa caaagacctg ttaatctgga cctacagacc
     2161 atccggttcc ccatcacggc gatagcgtcc attctccatc gcgtttccgg tgtgatcacc
     2221 tttgttgcag tgggcatcct gctgtggctt ctgggtacca gcctctcttc ccctgaaggt
     2281 ttcgagcaag cttccgcgat tatgggcagc ttcttcgtca aatttatcat gtggggcatc
     2341 cttaccgctc tggcgtatca cgtcgtcgta ggtattcgcc acatgatgat ggattttggc
     2401 tatctggaag aaacattcga agcgggtaaa cgctccgcca aaatctcctt tgttattact
     2461 gtcgtgcttt cacttctcgc aggagtcctc gtatggtaag caacgcctcc gcattaggac
     2521 gcaatggcgt acatgatttc atcctcgttc gcgctaccgc tatcgtcctg acgctctaca
     2581 tcatttatat ggtcggtttt ttcgccacca gtggcgaact gacatatgaa gtctggatcg
     2641 gtttcttcgc ctctgcgttc accaaagtgt tcaccctgct ggcgctgttt tctatcttga
     2701 ttcatgcctg gatcggcatg tggcaggtgt tgaccgacta cgttaaaccg ctggcattgc
     2761 gcctgatgct gcaactggtg attgtcgttg cactggtggt ttacgtgatt tatggattcg
     2821 ttgtggtgtg gggtgtgtga tgaaattgcc agtcagagaa tttgatgcag ttgtgattgg
     2881 tgccggtggc gcaggtatgc gcgcggcgct gcaaatttcc cagagcggcc agacctgtgc
     2941 gctgctctct aaagtcttcc cgacccgttc ccataccgtt tctgcgcaag gcggcattac
     3001 cgttgcgctg ggtaataccc atgaagataa ctgggaatgg catatgtacg acaccgtaaa
     3061 agggtcggac tatatcggtg accaggacgc gattgaatat atgtgtaaaa ccgggccgga
     3121 agcgattctg gaactcgaac acatgggcct gccgttctcg cgtcttgatg atggtcgtat
     3181 ctatcaacgt ccgtttggcg gtcagtcgaa aaacttcggc ggcgagcagg cggcacgcac
     3241 tgcggcggcg gctgaccgta ccggtcacgc actgttgcac acgctttatc agcagaacct
     3301 gaaaaaccac accaccattt tctccgagtg gtatgcgctg gatctggtga agaaccagga
     3361 tggcgcggtg gtgggttgta ctgcactgtg catcgaaact ggtgaagtgg tttatttcaa
     3421 agcccgcgct accgtgctgg cgactggcgg ggcagggcgt atttatcagt ccaccactaa
     3481 cgcccacatt aacactggcg acggcgtcgg catggctatc cgcgcaggcg taccggtgca
     3541 ggatatggaa atgtggcagt tccacccgac cggtattgcc ggtgcgggcg tactggtcac
     3601 cgaaggttgc cgtggtgaag gcggttatct gctgaacaaa catggcgaac gctttatgga
     3661 gcgttatgcg ccgaacgcca aagacctggc gggccgtgat gtggtggcgc gttccatcat
     3721 gatcgaaatc cgtgaaggtc gcggctgtga tggtccgtgg gggccacacg cgaaactgaa
     3781 actcgaccac ctgggtaaag aagttctcga atcccgtctg ccgggcatcc tggaactctc
     3841 ccgtaccttc gctcacgtcg atccggtgaa agagccgatc ccggttatcc caacctgtca
     3901 ttacatgatg ggcggtattc cgaccaaagt gaccggtcag gcgctgactg tgaatgagaa
     3961 aggcgaagat gtggttgttc cgggactgtt tgccgttggt gaaatcgctt gtgtatcggt
     4021 acacggcgct aaccgtctgg gcggcaactc gctgctggac ctggtggtct ttggtcgtgc
     4081 ggcaggtctg catctgcaag agtctatcgc cgagcagggc gcattgcgcg atgccagcga
     4141 gtctgatgtt gaagcatctc tggatcgtct gaaccgctgg aacaataatc gtaacggtga
     4201 agatccggtg gcgattcgta aagcgctgca agaatgtatg cagcataact tctcggtctt
     4261 ccgtgaaggt gatgcgatgg cgaaagggct tgagcagttg aaagttatcc gcgagcgtct
     4321 gaaaaatgcc cgtctggatg acacttccag cgagttcaac acccagcgcg ttgagtgcct
     4381 ggaactggat aacctgatgg aaacggcgta tgcaacggct gtttctgcca acttccgtac
     4441 cgaaagccgt ggcgcgcata gccgcttcga cttcccggat cgtgatgatg aaaactggct
     4501 gtgccactcc ctgtatctgc cagagtcgga atccatgacg cgccgaagcg tcaacatgga
     4561 accgaaactg cgcccggcat tcccgccgaa gattcgtact tactaatgcg gagacaggaa
     4621 aatgagactc gagttttcaa tttatcgcta taacccggat gttgatgatg ctccgcgtat
     4681 gcaggattac accctggaag cggaagaagg tcgcgacatg atgctgctgg atgcgcttat
     4741 ccagctgaaa gagaaagatc ccagcctgtc gttccgccgc tcctgtcgtg aaggtgtgtg
     4801 cggttccgac ggtctgaaca tgaacggcaa gaatggtctg gcctgtatta ccccgatttc
     4861 ggcactcaac cagccgggca agaagattgt gattcgcccg ctgccaggtt taccggtgat
     4921 ccgcgatttg gtggtagaca tgggacaatt ctatgcgcaa tatgagaaaa ttaagcctta
     4981 cctgttgaat aatggacaaa atccgccagc tcgcgagcat ttacagatgc cagagcagcg
     5041 cgaaaaactc gacgggctgt atgaatgtat tctctgcgca tgttgttcaa cctcttgtcc
     5101 gtctttctgg tggaatcccg ataagtttat cggcccggca ggcttgttag cggcatatcg
     5161 tttcctgatc gatagccgtg ataccgagac tgacagccgc ctcgacggtt tgagcgatgc
     5221 attcagcgta ttccgctgtc acagcatcat gaactgcgtc agtgtatgtc cgaaggggct
     5281 gaacccgacg cgcgccatcg gccatatcaa gtcgatgttg ttgcaacgta atgcgtaaac
     5341 cgtaggcctg ataagacgcg caagcgtcgc atcaggcaac cagtgccgga tgcggcgtga
     5401 acgccttatc cggcctacaa gtcattaccc gtaggcctga taagcgcagc gcatcaggcg
     5461 taacaaagaa atgcaggaaa tctttaaaaa ctgcccctga cactaagaca gtttttaaag
     5521 gttccttcgc gagccactac gtagacaaga gctcgcaagt gaaccccggc acgcacatca
     5581 ctgtgcgtgg tagtatccac ggcgaagtaa gcataaaaaa gatgcttaag ggatcacgat
     5641 gcagaacagc gctttgaaag cctggttgga ctcttcttac ctctctggcg caaaccagag
     5701 ctggatagaa cagctctatg aagacttctt aaccgatcct gactcggttg acgctaactg
     5761 gcgttcgacg ttccagcagt tacctggtac gggagtcaaa ccggatcaat tccactctca
     5821 aacgcgtgaa tatttccgcc gcctggcgaa agacgcttca cgttactctt caacgatctc
     5881 cgaccctgac accaatgtga agcaggttaa agtcctgcag ctcattaacg cataccgctt
     5941 ccgtggtcac cagcatgcga atctcgatcc gctgggactg tggcagcaag ataaagtggc
     6001 cgatctggat ccgtctttcc acgatctgac cgaagcagac ttccaggaaa ccttcaacgt
     6061 cggttcattt gccagcggca aagaaaccat gaaactcggc gagctgctgg aagccctcaa
     6121 gcaaacctac tgcggcccga ttggtgccga gtatatgcac atcaccagca ccgaagaaaa
     6181 acgctggatc caacagcgta tcgagtctgg tcgcgcgact ttcaatagcg aagagaaaaa
     6241 acgcttctta agcgaactga ccgccgctga aggccttgaa cgttacctcg gcgcaaaatt
     6301 ccctggcgca aaacgcttct cgctggaagg cggtgacgcg ttaatcccga tgcttaaaga
     6361 gatgatccgc cacgctggca acagcggcac ccgcgaagtg gttctcggga tggcgcaccg
     6421 tggtcgtctg aacgtgctgg tgaacgtgct gggtaaaaaa ccgcaagact tgttcgacga
     6481 gttcgccggt aaacataaag aacacctcgg cacgggtgac gtgaaatacc acatgggctt
     6541 ctcgtctgac ttccagaccg atggcggcct ggtgcacctg gcgctggcgt ttaacccgtc
     6601 tcaccttgag attgtaagcc cggtagttat cggttctgtt cgtgcccgtc tggacagact
     6661 tgatgagccg agcagcaaca aagtgctgcc aatcaccatc cacggtgacg ccgcagtgac
     6721 cgggcagggc gtggttcagg aaaccctgaa catgtcgaaa gcgcgtggtt atgaagttgg
     6781 cggtacggta cgtatcgtta tcaacaacca ggttggtttc accacctcta atccgctgga
     6841 tgcccgttct acgccgtact gtactgatat cggtaagatg gttcaggccc cgattttcca
     6901 cgttaacgcg gacgatccgg aagccgttgc ctttgtgacc cgtctggcgc tcgatttccg
     6961 taacaccttt aaacgtgatg tcttcatcga cctggtgtgc taccgccgtc acggccacaa
     7021 cgaagccgac gagccgagcg caacccagcc gctgatgtat cagaaaatca aaaaacatcc
     7081 gacaccgcgc aaaatctacg ctgacaagct ggagcaggaa aaagtggcga cgctggaaga
     7141 tgccaccgag atggttaacc tgtaccgcga tgcgctggat gctggcgatt gcgtagtggc
     7201 agagtggcgt ccgatgaaca tgcactcttt cacctggtcg ccgtacctca accacgaatg
     7261 ggacgaagag tacccgaaca aagttgagat gaagcgcctg caggagctgg cgaaacgcat
     7321 cagcacggtg ccggaagcag ttgaaatgca gtctcgcgtt gccaagattt atagcgatcg
     7381 ccaggcgatg gctgccggtg agaaactgtt cgactggggc ggtgcggaaa acctcgctta
     7441 cgccacgctg gttgacgaag gcattccggt tcgcctgtcg ggtgaagact ccggtcgcgg
     7501 taccttcttc caccgccacg cggtgatcca caaccagtct aacggttcca cttacacgcc
     7561 gctgcaacac atccataacg ggcagggcgc gttccgcgtc tgggactccg tactgtctga
     7621 agaagcagtg ctggcgtttg aatacggtta tgccaccgca gaaccacgca ctctgaccat
     7681 ctgggaagcg cagttcggtg acttcgctaa cggtgctcag gtggttatcg accagttcat
     7741 ctcctctggc gaacagaaat ggggccggat gtgtggtctg gtgatgttgc tgccgcacgg
     7801 ttatgaaggg caggggccgg agcactcctc cgcgcgtctg gaacgttatc tgcaactttg
     7861 tgctgagcaa aacatgcagg tgtgcgtacc gtctaccccg gcacaggttt accacatgct
     7921 gcgtcgtcag gcgctgcgcg ggatgcgtcg tccgctggtc gtgatgtcgc cgaaatccct
     7981 gctgcgtcat ccgctggcgg tttccagcct cgaagaactg gcgaacggca ccttcctgcc
     8041 agccatcggt gaaatcgacg agcttgatcc gaagggcgtg aagcgcgtag tgatgtgttc
     8101 tggtaaggtt tattacgacc tgctggaaca gcgtcgtaag aacaatcaac acgatgtcgc
     8161 cattgtgcgt atcgagcaac tctacccgtt cccgcataaa gcgatgcagg aagtgttgca
     8221 gcagtttgct cacgtcaagg attttgtctg gtgccaggaa gagccgctca accagggcgc
     8281 atggtactgc agccagcatc atttccgtga agtgattccg tttggggctt ctctgcgtta
     8341 tgcaggccgc ccggcctccg cctctccggc ggtagggtat atgtccgttc accagaaaca
     8401 gcaacaagat ctggttaatg acgcgctgaa cgtcgaataa ataaaggata cacaatgagt
     8461 agcgtagata ttctggtccc tgacctgcct gaatccgtag ccgatgccac cgtcgcaacc
     8521 tggcataaaa aacccggcga cgcagtcgta cgtgatgaag tgctggtaga aatcgaaact
     8581 gacaaagtgg tactggaagt accggcatca gcagacggca ttctggatgc ggttctggaa
     8641 gatgaaggta caaccgtaac gtctcgtcag atccttggtc gcctgcgtga aggcaacagc
     8701 gccggtaaag aaaccagcgc caaatctgaa gagaaagcgt ccactccggc gcaacgccag
     8761 caggcgtctc tggaagagca aaacaacgat gcgttaagcc cggcgatccg tcgcttgctg
     8821 gctgaacata acctcgacgc cagcgctatt aaaggcaccg gcgtgggtgg tcgtctgacc
     8881 cgtgaagatg tggaaaaaca tctggcgaaa tccccggcga aagagtctgc accggcagcg
     8941 gctgctccgg cggcgcaacc ggctctggct gcacgtagtg aaaaacgtgt cccgatgact
     9001 cgcctgcgta agcgtgtggc agagcgtctg ctggaagcga aaaactccac cgccatgctg
     9061 accacgttca acgaagtcaa catgaagccg attatggatc tgcgtaagca gtacggtgaa
     9121 gcgtttgaaa aacgccacgg catccgtctg ggctttatgt ccttctacgt gaaagcggtg
     9181 gttgaagccc tgaaacgtta cccggaagtg aatgcttcta tcgacggcga tgacgtggtt
     9241 taccacaact atttcgacgt cagcatggcg gtttctacgc cgcgcggcct ggtgacgccg
     9301 gttctgcgtg atgtcgatac cctcggcatg gcagacatcg agaagaaaat caaagagctg
     9361 gcagtcaaag gccgtgatgg caagctgacc gttgaagatc tgaccggtgg taacttcacc
     9421 atcaccaacg gtggtgtgtt cggttccctg atgtctacgc cgatcatcaa cccgccgcag
     9481 agcgcaattc tgggtatgca cgctatcaaa gatcgtccga tggcggtgaa tggtcaggtt
     9541 gagatcctgc cgatgatgta cctggcgctg tcctacgatc accgtctgat cgatggtcgc
     9601 gaatccgtgg gcttcctgat aacgatcaaa gagttgctgg aagatccgac gcgtctgctg
     9661 ctggacgtgt agtagtttaa gtttcacctg cactgtagac aggataaggc attatcgcct
     9721 tctccggcaa ttgaagcctg atgcgacgct taacgcgtct tatcaggcct acgggaagca
     9781 ccaatgtagg tcggataagg cgcttgcgcc gcatccgaca agcgatgcct gatgcgacac
     9841 ttacgcgtct tatcaggcct acaggtgacc gacaatgccc ggaggcgata cgaaatattc
     9901 ggtctacggt ttaaaagata acgattactg aaggatggac agaacacatg aacttacatg
     9961 aatatcaggc aaaacaactt tttgcccgct atggcttacc agcaccggtg ggttatgcct
    10021 gtactactcc gcgcgaagca gaagaagccg cttcaaaaat cggtgccggt ccgtgggtag
    10081 tgaaatgtca ggttcacgct ggtggccgcg gtaaagcggg cggtgtgaaa gttgtaaaca
    10141 gcaaagaaga catccgtgct tttgcagaaa attggctcgg caagcgtctg gtaacgtatc
    10201 aaacagatgc caatggccaa ccggttaacc agattctggt tgaagcagcg accgatatcg
    10261 ctaaagagct gtatctcggt gccgttgtag accgtagttc ccgtcgtgtg gtctttatgg
    10321 cctccactga aggcggcgtg gaaatcgaaa aagtggcgga agaaactccg cagctgatcc
    10381 ataaagttgc gcttgatccg ctgacaggcc cgatgccgta tcagggacgc gagctggcgt
    10441 tcaaactggg tctggaaggt aaactggttc agcagttcac caaaatcttc atgggtctgg
    10501 cgaccatttt cctggagcgc gacctggcgt tgatcgaaat caacccgctg gtcatcacca
    10561 aacagggtga tctgatttgc ctcgacggca aactgggcgc tgatggcaac gcactgttcc
    10621 gccagcttga tctgcgcgaa atgcgtgacc agtcgcagga agatccgcgt gaagcacagg
    10681 ctgcacagtg ggaactgaac tacgttgcgc tggacggtaa catcggttgt atggttaacg
    10741 gcgcaggtct ggcgatgggt acgatggaca tcgttaaact gcacggcggc gaaccggcta
    10801 acttccttga cgttggcggc ggtgcaacca aagaacgtgt aaccgaagcg ttcaaaatca
    10861 tcctctctga cgacaaagtg aaagtcgttc tggttaacat cttcggcggt atcgttcgtt
    10921 gcgacctgat cgctgacggt atcatcggcg cggtagcaga agtgggtgtt aacgtaccgg
    10981 tcgtggtacg tctggaaggt aacaacgccg aactcggcgc gaagaaactg gctgacagcg
    11041 gcctgaatat tattgcagca aaaggtctga cggatgcagc tcagcaggtt gttgccgcag
    11101 tggaggggaa ataatgtcca ttttaattga taaaaacacc aaggttatct gccagggctt
    11161 tactggtagc caggggactt tccactcaga acaggccatt gcatacggca ctaaaatggt
    11221 tggcggcgta accccaggta aaggcggtac tacccacctc ggcctgccgg tgttcaacac
    11281 cgtgcgtgaa gctgttgctg ctactggcgc taccgcttct gttatctacg taccagcacc
    11341 gttctgcaaa gactccattc tggaagctat tgatgcaggc atcaaactga ttatcaccat
    11401 cactgaaggc atcccgacgc tggatatgct gactgtgaaa gtgaaactgg acgaagccgg
    11461 cgttcgtatg atcggcccga actgcccggg cgttatcact ccgggtgaat gcaaaatcgg
    11521 tatccagccg ggtcacattc acaaaccggg taaagtgggt atcgtttccc gttccggtac
    11581 actgacctat gaagcggtta aacagaccac ggattacggt ttcggtcagt cgacctgtgt
    11641 tggtatcggc ggtgacccga tcccaggctc taactttatc gacatcctcg aaatgttcga
    11701 aaaagatccg cagaccgaag cgatcgtgat gatcggtgag atcggcggta gcgctgaaga
    11761 agaagcggca gcgtacatca aagagcacgt taccaagcca gttgtaggtt acatcgctgg
    11821 tgtgactgcg ccgaaaggca aacgtatggg ccacgcgggt gcgatcattg ccggtgggaa
    11881 agggactgcg gatgagaaat tcgctgctct ggaagccgca ggcgtgaaaa ccgttcgcag
    11941 cctggcggat atcggtgaag cactgaaaac tgttctgaaa taaatatctg taataagaaa
    12001 tagccctcgc cgcttccctc tacaggaatg gcgaagggct gtcggtttcg acatggttgg
    12061 ccatcgtatg atggcctttt ttgtgcttat cgcgatgatt ttcgctgcgc tatcagggta
    12121 aatttatagt catcggtatt aaaagcgttg cggctatatt caaacacccg accatcaact
    12181 aaatatccac gcgatacttt ttcaagaatc ggctttgtct ggctgatatt aagcagacgg
    12241 ctcatctctt cggttggcat cagaggaatg atttcctgtt cgctacgatc gataaccatc
    12301 ttcttcactt cttcaataaa gtgatatttc gaattttcca tgacctgcca ggtgagatcc
    12361 gggaacaacg caagcggcat ccaggtttct tccagcgcca ttggcttttg cttgcgatag
    12421 cgcacgcgct tcacatgcca cacacgatcc tgcgcggtga tttgtagctg ttgctgaaga
    12481 aaatcgtcag ccggaatcac ttcaaatatc aggacttcac tgtgtgtatc gacgtgacgg
    12541 tccgacagtt tttcatcaaa actggttaac tgaaaaatat cgtaattgac ccgctcttct
    12601 ttgacgtaag tcccgctgcc ctgaatgctt tcgaggatct gctgctcgac tagctggcgc
    12661 aaagcctgac gcaccgtaac ccggctgacg ccaaactctg tttgtagcgc tgattcagtg
    12721 ggtaacgcat cgccaggttt aagctcgcca cgcgcaattt gttcacgaat gcgatcggca
    12781 atctgccggt ataagggctt gtgtcccatt tttagtatct cattaatacg aatttagcca
    12841 ttatgcccga taaattcatc ctgtaaataa tgcaaataca atacaaataa tttcaatcaa
    12901 gtgaaattga tcatataatg gtattgtttt atcgggcaca ctggcgcgac tataaaaacg
    12961 atcaagtgag gatcatgatg aatctgacga ctctgaccca ccgcgatgcg ttgcgtctga
    13021 atgcgcgctt taccagccgt gaagaggcca tccacgcgtt gactcaacgt cttgctgctc
    13081 tggggaaaat ttccagtact gagcaatttc tggaagaagt gtatcgccgt gaaagccttg
    13141 gcccgaccgc cttaggtgaa gggttggctg tgccgcatgg caaaactgct gcggtaaaag
    13201 aagcggcgtt tgcggtcgcc acactcagcg agccgcttca gtgggaaggc gttgatggcc
    13261 cggaagcagt tgatttagtg gtgctgctgg cgattccccc caatgaagcg ggtactatgc
    13321 atatgcaact gctgacagcg ctgaccacgc gccttgcgga tgatgagatt cgggcgcgta
    13381 tacagtcggc gacgacgcct gatgagttgc tctcggcgct ggatgacaag ggaggcacgc
    13441 aaccttctgc ctctttttcc aacgcgccaa ctatcgtctg cgtaccggcc tgtccggcgg
    13501 gtattgctca cacctatatg gctgcggaat atctggaaaa agccggacgc aaactcggcg
    13561 taaatgttta cgttgaaaaa caaggcgcta acggcattga agggcgttta acggcggatc
    13621 aactcaatag tgcaaccgcc tgtatttttg cggctgaagt cgccatcaag gagagtgagc
    13681 gttttaatgg cattcccgcg ctttcagtgc ctgttgccga gccgattcgc catgcagaag
    13741 cgttgatcca acaagcgctt accctcaagc gtagcgatga gacgcgtacc gtacagcaag
    13801 atacgcaacc ggtgaaaagt gtcaaaacgg agctgaaaca ggcactgttg agcggaatct
    13861 cttttgccgt accgttgatt gtcgcggggg gcacggtgct ggcggtcgcg gtattactgt
    13921 cgcaaatctt cgggctacaa gatctgttta atgaagaaaa ctcctggctg tggatgtacc
    13981 gcaagctggg cggcgggctg ctcggaattt tgatggtacc ggtgctcgcg gcctataccg
    14041 cctattctct ggcagataaa ccggcgttag cgccaggctt tgcggctgga cttgccgcca
    14101 acatgatcgg ctccgggttt ctcggcgcgg tcgttggcgg attgatagcc ggttacttga
    14161 tgcgctgggt gaaaaatcac ttgcgtctta gcagtaaatt caatggattc ctgacttttt
    14221 atctctaccc ggtgctcggt acgttgggag cgggcagtct gatgctgttt gtggtgggag
    14281 aacctgtcgc atggatcaat aactcgctta ccgcctggct gaacggtctg tcaggaagta
    14341 acgcgctgtt gctgggtgcc attttcggtt ttatgtgttc ctttgatctt ggagggccag
    14401 tgaacaaagc cgcttatgca ttctgcctgg gcgcaatggt gaacggcgtt tacggcccgt
    14461 atgccatttt cgcctccgtc aaaatggttt cggcatttac cgtaaccgct tccacgatgc
    14521 tcgcaccgcg cctgtttaaa gagtttgaaa ttgagaccgg gaaatccacc tggctgttag
    14581 ggctggtagg tattaccgaa ggggcgatcc cgatggcgat tgaagatccg ctgcgggtta
    14641 ttggttcgtt tgtgctgggc tctatggtaa cgggcgctat tgtcggtgcg atgaatatcg
    14701 gcctttcgac accctgtgcc ggcattttct cgctcttttt acttcatgat aatggcgcgg
    14761 gcggtgttat ggcggcaatt ggctggtttg gtgcggcatt ggtgggggct gcaatctcga
    14821 ctgcaattct cctgatctgg cggcgtcacg cggttaagca tggcaactat ctgactgatg
    14881 gcgtaatgcc ataaacaaaa aggaaacgac gatgaaagcg gtatctcgcg ttcacatcac
    14941 cccgcatatg cactgggatc gagagtggta tttcaccacc gaagagtcac gtattctgct
    15001 ggtcaataat atggaagaga tcctgtgccg actggaacag gacaacgaat acaaatatta
    15061 cgtactcgac gggcaaacgg cgatcctcga agattatttc gcggtgaaac cggaaaacaa
    15121 agaccgtgtg aagaaacagg tagaagccgg caagttgatt atcggcccct ggtataccca
    15181 gaccgatacc acgattgttt ctgcggaatc catcgtccgt aatctgatgt acggaatgcg
    15241 tgactgcctc gcgtttggcg agccgatgaa aataggttat ttaccagatt cctttggcat
    15301 gtccgggcaa ctgccgcata tctacaatgg atttggcatt acccgcacca tgttctggcg
    15361 cggatgttcg gagcgccacg gtactgataa aaccgagttt ttgtggcaaa gcagtgacgg
    15421 tagcgaagtg acggcgcagg tgctgccgct gggctacgcc ataggtaagt acttacctac
    15481 cgacgaaaac ggattacgta aacgcctcga cagttatttt gacgtgctgg aaaaagcgtc
    15541 tgtaaccaaa gagattttgc tgccgaatgg tcatgaccag atgccattgc agcaaaatat
    15601 cttcgaagtg atggataagc tacgtgagat ctaccctcaa cgtaagtttg tgatgagccg
    15661 ctttgaagag gtatttgaga agatcgaagc gcagcgtgaa agtctggcca ccctgaaagg
    15721 tgaatttatc gatggcaaat atatgcgcgt acatcgcacc atcggttcca cgcgcatgga
    15781 tatcaaaatt gcccacgcac gtattgaaaa taagattgtt aatctgctgg aaccgctggc
    15841 aacgctggcc tggacgttgg gttttgaata ccaccacggc ttgctggaga aaatgtggaa
    15901 agagatctta aaaaatcatg cccacgacag tatcggttgt tgttgcagtg acaaagttca
    15961 tcgcgaaatt gtcgcccgct tcgaactggc tgaagacatg gcggataatc tgctgcgttt
    16021 ctacatgcgc aaaattgccg ataacatgcc acagagtgat gccgacaaac tcgtgctgtt
    16081 taacctgatg ccctggccgc gggaagaagt tattaacacc actgtgcggc tgcgcgccag
    16141 ccagtttaat ctacgagacg atcgcggtca gcctgtaccg tattttattc gccatgcccg
    16201 tgagatcgat ccaggcctaa tcgatcggca aatagttcat tacggtaatt acgatccctt
    16261 tatggagttt gatatacaga tcaaccagat tgtcccttct atgggctatc gcacgcttta
    16321 tatcgaagcg aatcagcctg gcaacgtgat tgcggcaaaa agtgacgctg aagggatact
    16381 ggaaaatgct ttctggcaaa ttgcgctcaa tgaggatggt tctctgcaac tggtagataa
    16441 agacagcggt gtgcgctatg accgagtatt gcaaa
//
LOCUS       DAZPEB010000085        16408 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZPEB010000085 DAZPEB010000000
VERSION     DAZPEB010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16408
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.086"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(107..571)
                     /gene="nanQ"
                     /locus_tag="ACHURJ_002323"
     CDS             complement(107..571)
                     /gene="nanQ"
                     /locus_tag="ACHURJ_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFR1281252.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIDNEQAIILKPGMFAVFMPSEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(568..1443)
                     /gene="nanK"
                     /locus_tag="ACHURJ_002324"
     CDS             complement(568..1443)
                     /gene="nanK"
                     /locus_tag="ACHURJ_002324"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFR1281253.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPQALRDA
                     LAALVAPLQAHAQQVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLSNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELMGADARTIFTRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1440..2129)
                     /locus_tag="ACHURJ_002325"
     CDS             complement(1440..2129)
                     /locus_tag="ACHURJ_002325"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFR1281254.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2177..3667)
                     /gene="nanT"
                     /locus_tag="ACHURJ_002326"
     CDS             complement(2177..3667)
                     /gene="nanT"
                     /locus_tag="ACHURJ_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFR1281255.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVSGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(3776..4669)
                     /gene="nanA"
                     /locus_tag="ACHURJ_002327"
     CDS             complement(3776..4669)
                     /gene="nanA"
                     /locus_tag="ACHURJ_002327"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFR1281256.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(4791..5582)
                     /gene="nanR"
                     /locus_tag="ACHURJ_002328"
     CDS             complement(4791..5582)
                     /gene="nanR"
                     /locus_tag="ACHURJ_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFR1281257.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            5962..7328
                     /gene="dcuC"
                     /locus_tag="ACHURJ_002329"
                     /pseudo
     CDS             5962..7328
                     /gene="dcuC"
                     /locus_tag="ACHURJ_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
     gene            complement(7371..7868)
                     /gene="sspB"
                     /locus_tag="ACHURJ_002330"
     CDS             complement(7371..7868)
                     /gene="sspB"
                     /locus_tag="ACHURJ_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFR1281258.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(7874..8512)
                     /gene="sspA"
                     /locus_tag="ACHURJ_002331"
     CDS             complement(7874..8512)
                     /gene="sspA"
                     /locus_tag="ACHURJ_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFR1281259.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(8907..9299)
                     /gene="rpsI"
                     /locus_tag="ACHURJ_002332"
     CDS             complement(8907..9299)
                     /gene="rpsI"
                     /locus_tag="ACHURJ_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFR1281260.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(9315..9743)
                     /gene="rplM"
                     /locus_tag="ACHURJ_002333"
     CDS             complement(9315..9743)
                     /gene="rplM"
                     /locus_tag="ACHURJ_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFR1281261.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKCTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(9961..11088)
                     /gene="zapE"
                     /locus_tag="ACHURJ_002334"
     CDS             complement(9961..11088)
                     /gene="zapE"
                     /locus_tag="ACHURJ_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFR1281262.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGAENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            11282..11680
                     /gene="zapG"
                     /locus_tag="ACHURJ_002335"
     CDS             11282..11680
                     /gene="zapG"
                     /locus_tag="ACHURJ_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFR1281263.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            11834..13201
                     /gene="degQ"
                     /locus_tag="ACHURJ_002336"
     CDS             11834..13201
                     /gene="degQ"
                     /locus_tag="ACHURJ_002336"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFR1281264.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDCGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            13291..14358
                     /gene="degS"
                     /locus_tag="ACHURJ_002337"
     CDS             13291..14358
                     /gene="degS"
                     /locus_tag="ACHURJ_002337"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFR1281265.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGVNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(14421..15359)
                     /gene="mdh"
                     /locus_tag="ACHURJ_002338"
     CDS             complement(14421..15359)
                     /gene="mdh"
                     /locus_tag="ACHURJ_002338"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFR1281266.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            15794..16264
                     /gene="argR"
                     /locus_tag="ACHURJ_002339"
     CDS             15794..16264
                     /gene="argR"
                     /locus_tag="ACHURJ_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFR1281267.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT         4050 a         4282 c         4116 g         3960 t
ORIGIN      
        1 tgttaacata aaagaataca acgtcaggta atggcacaga gcggtccgtc agttcaggtt
       61 tggctctgtg ccaaagatgg gtcaggtcac acctgatcta ataaatttaa gccattaaat
      121 cagccttaac cttcaccacc acctttttaa tctcacccgg ctcgccgaca acgcatcctg
      181 gtttatgcgg ttcacttggc ataaacacgg cgaacattcc cggttttaag atgatggctt
      241 gctcgttatc aatggtgctg caaagctgat aatcatcctc atggtggaac tcttcacact
      301 ggcgcgcagt gcctgccatg ccaaacagaa tccgttcctc accgtttaat aacagctgga
      361 tatcaatgta ttgctcgtgc aattccgctt ttttctcgac gggcgattga gtgttaaacg
      421 tcatgacatt cataaagata ttgtcgccct gtaattcgta acgaccaggc gctttttctt
      481 gcggtctggc agctaatgcc agcgttaacg cgtcctgtaa cgcaggatgt aacccagcag
      541 acggtaatga ctgtacttca cccatcatca taatttttct ccctgggcca acagcgcagc
      601 cccaagtaaa cctgcatcgt ggcggtagtg cgccgccagt aaatcaacat gaaatgccgc
      661 tggctcctgc gccagatacg tttccaccaa cgccagatac ccttctgcca gaccaacgct
      721 gccaccgacc accacgcact ggcaatcagt tgtggcttta atatcagcga tcagccttgc
      781 cagcacatgt gcggagcggt gaatcagttg ctgcgcctgc tcgtcgccct gcccggcgcg
      841 tgtgaaaata gttctcgcat ccgcgcccat gagttcacct tgcgctgccg ctgcaatacc
      901 gcgaccagaa gcaatcgctt ccacgcaacc tgtgcgtcca cagccgcaga ctgggccgtg
      961 tggatcggca agcgtatgcc cgatgtgccc cgccagaccg ccagggccgg taagcagttt
     1021 accgccgctc actacaccgc cgccgacgcc agtggaaacg gtgataaaca ccatatcggt
     1081 tatatcgcct tccagcgcct gatactctgc ccatgctgcg gcctgcgcgt cgttaatggc
     1141 aatggtcggc aaattggaaa gttgttccag cgttttgact aacggaaagt gtagcaatcc
     1201 accaagatta tgcggattca gcgccagcaa gctgccgtcg cgaataattc cggttgaagc
     1261 gatggcaacc tgctgcgcat gagcttgcaa cggggcgact aacgcggcta aggcatcacg
     1321 caacgcctgc ggagtctggc tggctggtgt cggaagttct cggcgatcgc ggatctgccc
     1381 gtcagcgcca atcagcgcgg cggcaagttt agtaccgccg atatcaatcg ccaacgtggt
     1441 catagcaccg cctttttcat cgctgtgttg taccactgac aaatgtgttc aagacgcgta
     1501 atggcagaac cgaccgtcac cgcccacgcg ccgtggcgca tcgcatctgc cgcctgagca
     1561 ggcgtgttgt aacgcccttc ggcaatcacc cgacatccgg cgtcgctcaa cgttttcacc
     1621 agcgccagat ccggctcttc tggcgtttca ggcgtggtat agccagaaag cgtagtgcca
     1681 ataatttcgg ctcccagctt ttggcatgcc aggccgtctt ccggcgttga gcagtcggtc
     1741 atcgccagta aaccgtgatg gtgaatacgt gccagcagcg tttcaacagg caccggacgc
     1801 gggcggtcgg tgccgtcaat ggcgataatg tccgcgcccg cctgcgccag cgcatcaaca
     1861 tcttcaatat aggccgtgat gcgtaccgga gaatcctcca gatcgcgttt cacaattcca
     1921 ataatcggca cgctcaccac cgcacgcgtg gcttgcagat ttgccacacc ttcaatgcga
     1981 atggcaaccg cgcccgcctg ttctgccgct aatgccatgg cagcgacgat ttcgggttta
     2041 tcgagcgggc tgtccggaac cggctggcag gagacaatca ggccaccgtt agcagcgatt
     2101 ttttgatcca gttgtgcaag taacgacata catcttccct tagcgaaagg cccggtacag
     2161 agaccgggca acaggattaa cttttggttt tgactaaatc gtttttggcg ctgccaaatg
     2221 gcacggcacc gctaaatggt ttgccgtcga tagcgtcatg agtacgcaat gcttccgggc
     2281 gcagccaacg ctgaacgcgg gaaggcatat ccagaccaat cagcaggatc accacaaatg
     2341 tcagactgaa cgatagcgat gccagcgcag tacccagatc cagacgttga gcgatcaacg
     2401 cgccgatgat tggggccagt gcgccgccca atgcgccaac gttgtaggta aagcccaggc
     2461 ccgctgcacg ctggtcggta tcgaaataac cgccaatcag tttcggtaag atcccggcga
     2521 tcccttgtcc aagcatttgc tggaagaaca gtaacagacc gagcacccag acgtttgcgc
     2581 cgccaatcgc aaataccgga ataatcaaca gctgcgaggc caacaggcta caaacgtagg
     2641 ctttgcgggt tcccagccag tcaccgagga agccgctgac acagcacccc accgcagccc
     2701 caaagccgct aaagaacagc acattggcga cagtatgcgg gttataagcc agatcggttt
     2761 tcagatacgt tggcagcagc gcctgaatcg gccatgagta gaggaaagca aacaacacga
     2821 ccaccatcag cattacgccc gttggccagc gtttacccgt actttgcacc ataaagctga
     2881 taaagattgc ggcgcataac agcccaagaa cagcgacgat cgcggcattt tgcaggttac
     2941 cggcgaagca aaaccacagc gcagtagccg ccgccagtgt cattacgata ttggcaatgc
     3001 gatgttcacc acggtagaga atatctacca tcgtgcgtac tggtgcttta cctgcatgct
     3061 tctctttcca gtcttccgct tccgggatgt ttttacgcag ccagagagca aagatgatag
     3121 gcaaaatgcc gataaagaac agcgcacgcc agccccagac cggaaccacc aggctataga
     3181 cctgagcggc aacgacggcc cccacagaga agcctgaaat cagaaaacca ctggctttgt
     3241 tacgcagatg ttttggccag ctttcaatga cataggtggc gctggaaccg tattcacccg
     3301 ccatccccat gccgatgacc agacgcgcga taaacatggt gatgtagcct ggcgcaaagc
     3361 cgcaggccag cgtcccggcc gagaagagaa cgatgctggt gaccattgcc agacgacgcc
     3421 cgtagcggtc acccatagcg ccgagcatca ggccgccgaa ccagcgagag ataaaggctg
     3481 cggagatcag acttgccgcc tgcaccgtcg tcagcccgaa ttcaccctgt acttcggtga
     3541 gtaccagggc gattaaaacg aaatcaaaac cgtcaagcag atatcccaac caggcagcgg
     3601 aaaatgcgcg ccattgtgca cggttgagat ggcgatacca cgggatattc tgggttgtag
     3661 tactcattgt gagtctcccg cggtgggcga tgcccacacg ctttggtata aaaattgtag
     3721 ggtacagatg cgtttatttc ccctcacccg gtaggggcga gcgaggggaa acaactcacc
     3781 cgcgctcttg catcaactgc tgggccagcg ccttcagttc tggcagatat ttttcatcta
     3841 ccggtccaaa cggtttgcgg cacagcggca cagaaacgac atccatataa tggaggacag
     3901 ttttcaggcc gcggaatacg cccgttttga tcagtaaatc aatgacttta ttgcattcag
     3961 tttgcagttt ctgcgcggtc tggatatcgc cttctttcag cgccttaacg atcccctgat
     4021 agcgccagcc catgatgttg taggtactac cgataccacc atcagcgccc gccagcagac
     4081 cagaggcgaa gatttcgtcg taaccgttat agagcacaag atcaggatgt tcacgacgga
     4141 tctgctccat ctgatagaga tcgccagagg tctgtttcag cgcacctacg ccaggcaatg
     4201 taacaagtgt gttgatctga tccagggtca gttttacccc actcagggct ggaatgttgt
     4261 acaccaccat cggcaaacca tccgccgaat caataattgc ccgatagtga tcgcagtgtt
     4321 cttcaaagct gaaaggatag tagaacggcg tgacggcgga gacggcatcg aagccataac
     4381 gtttagccga tgccgcaagt tgttggcttt cggcggtgct gacgcaaccg acgtgggcga
     4441 tgagtttaat cttacctttc gcctcttcgg cgacgatttc cagtacctgt tcacgctcgg
     4501 aaaggctttg tacaaaggcc tcgccggtcg aaccacccac gtataaaccg tcgatgccct
     4561 gctgaatatt gaactgaacc aggcgacgca gactcgcttt atccagtgct tgttgttggt
     4621 caaaaggagt caggagtgca gccattacgc cacgtaaatt cgttgccata aatacctctg
     4681 aagtgatgct tgtctgataa acgatatacc tttatacctg ttataccaga tcaattaagc
     4741 aacaccccat acagaaagct tataatgcga tctgcttcac taaagtggca ttatttcttt
     4801 ttgttggtgg tctgaccgaa agcgtgccag gtagcagaga cgctgttgag atgcgattgc
     4861 aacgcacgat cggcttcgtc aggatcatga cggcggatcg catcaacgat cgcaatatgc
     4921 tgttgataac taacgttgtt atgttcgtgc agtgcctgat cggtaaccgt tgggcgtgcg
     4981 gcaataagcc agtcgagcag ggcaacgtgg atcgccatga agattgggtt accggggatc
     5041 tccgccagca cgcggtggaa atcaacgtct gaacgaatga atgccgcgtt gttatccagc
     5101 gactgactgt tgatttccag tgcttttgcc agcaaatcga tttgctcatc ggtggcatgt
     5161 tcagccgcat agcgcaccag actggattca aagaacagac gtaattgttc gaaatgggca
     5221 atcccaccgg gatgagaaag gaaatctttc gccatgccgg aaagctcacc gatgatagtg
     5281 tccgcagaag gacgcgagac gcgagcgcgt tcgccgttgt ttatttgcac cagacctttg
     5341 cgttttaacg ctgccagcgc ttcacgcacc gaaggacgcc cgacgttaaa gaacgccatc
     5401 agttcgcgtt cagacggtaa ttgttcacct tcgccaaatt cacgacggcg gatcatctgt
     5461 tccagctctt cttccaccat ttcggagagt tttttacgcg ccagcgggcg gctacgcaag
     5521 ttgcgaccaa ttgcaggtga agaatcttcg gtttgcgaat caaatgcgtt cataaggccc
     5581 attctgtaag gtcagtgtga ttaacatcat cagtgacatc ctatcacagg attgaaagta
     5641 ggggaaaatg gcaggttttt ctctttgtgc ctcatcatta ccataattaa cggaataatt
     5701 aactattgcg aaaaattaat gtaacgcaga taaaaacatc ccgtttgaat tatttataag
     5761 actattcacg agcattatga atattatgaa tgcgttcttg caaaataatc ataagcgcat
     5821 attttttaat gaaaaattat ctcacctaca attaaaaaca cgacatccgc accataaata
     5881 gacttgcaaa aaatataaca tcattgtttt caatctgccg tttatgggat tgaccgtttt
     5941 cttttgacac ggagttcaac aatgttcggc ataattatat ctgtcatcgt attaattacg
     6001 atgggctatt tgatcctgaa aaactacaaa cctcaggtgg tgctggctgc cgcaggtatc
     6061 ttcctgatga tgtgcggtgt ctggttaggg ttcggtggtg tactcgagcc cgccaaaagc
     6121 agcggctact tgatcgtcga tatttataat gaaatcctgc gcatgctgtc caaccgcatt
     6181 gccggattgg ggctatcgat tatggcggtg ggcggtatgc ccgctacatg gagcgcatag
     6241 gggccagtcg cgcgatggtg agcttgttaa gccgcccgtt aaaactcatt cgctcaccgt
     6301 atattattct gtcggcaact tacgtcatcg gtcaaatcat ggcgcagttt attaccagcg
     6361 cctccggtct gggtatgttg ctgatggtca ccttatttcc gacactggtg agtctgggag
     6421 taagtcgtct ctctgcggtg gcggttatcg caaccacaat gtccattgag tggggaattc
     6481 tggaaacgaa ctccattttt gcagcccagg tcgcgggaat gaaaattgcc acttacttct
     6541 tccactacca gcttccggtc gcctcttgcg tcattatctc ggtggcgatc tcccactttt
     6601 tcgtgcaacg cgcttttgac aaaaaagata aaaatatcaa tcacgaacaa gcagagcaaa
     6661 aagctctcga taatgtcccg ccgctctatt acgccatttt acctgtgatg ccgttaatcc
     6721 tgatgctcgg ctcgctgttc ctcgcccacg tcgggctgat gcagtcagaa ctgcatctgg
     6781 tggtggtgat gttactgagt ttgacggtga cgatgtttgt tgagttcttc cgcaagcata
     6841 acttgcgcga aacaatggac gatgtgcagg cgttttttga cggcatgggt acgcagtttg
     6901 ccaacgtggt aacgctggtg gtcgcgggtg aaatatttgc gaaaggctta acgacgattg
     6961 gcactgtcga tgcggttatc aggggtgcgg agcattctgg tctgggcggt attggcgtga
     7021 tgattattat ggcgctggtc attgccattt gtgccattgt gatgggctct ggcaatgcgc
     7081 cgtttatgtc atttgccagt cttattccga atatcgcagc tggactacat gtaccagcgg
     7141 ttgtaatgat tatgccgatg cattttgcca cgacgctagc gcgcgcggtt tcgccgatta
     7201 ctgcggtggt ggtcgttacg tcaggaattg caggcgtttc gccttttgcg gtggtgaagc
     7261 gaacagcgat ccccatggca gtcggtttcg tggtgaatat gattgccaca atcacgctat
     7321 tttattaagc cattaaaaga caaaacaggc caccagggcc tgtttttgta ttacttcaca
     7381 acgcgtaatg ccggtcgacc accgcgtggt ggctgcggag gttcatcgtc aggatgagtg
     7441 tcatcatcgt gatctggctt gtcgccatca ataaccgaca taacggtttc gttgtctgcc
     7501 gatgcctctt catcattcat gatgctggta tcttcatcgt aggcagcttc aggctcaaac
     7561 attgtgcctg cgccattttc acgggcgtag atagccagca cggcagccag cggcacagaa
     7621 acctgacgcg gaatgccgcc aaagcgcgcg ttaaagcgca cctcatcatt cgccagttcc
     7681 agattgccga cagcacgcgg cgcaatgttg agtacgattt gcccgtcacg cgcatattcc
     7741 ataggaacct gcacgccagg gagcgtcaca tccaccacca ggtgcggcgt gagctggtta
     7801 tccagcaacc actcatagaa tgcacgcagc agatagggac gacgtggtgt tagctgtgac
     7861 aaatccatac agattaactc cggcccagac gcatttcacg ttctgcttca gttaaagaag
     7921 caaggaaaga gtcacgctca aagacgcggg tcatatagcc tttcagctct ttcgcacccg
     7981 ggccgctgaa ctcgatgccc agttgcggca gacgccacag cagcggagca agatagcaat
     8041 cgaccaggct gaactcatcg ctcaggaagt acggcttctg accgaagacc ggcgcaatcg
     8101 ccagcagttc ttcgcgcagt tgcttacgtg cggcatctgc ttcagaagct gaaccgttga
     8161 tgatggtgtt catcagcgtg taccagtctt tttcgatgcg atgcatgtac agacggcttt
     8221 caccgcgagc taccgggtaa acaggcatca gtggcggatg cgggaaacgc tcatccagat
     8281 attccataat gatgcgagat tcccacaggg tcagctcacg atccaccagg gtcggaacgc
     8341 tctgattcgg gttgaggtca atcagatcct gaggcggatt gtccttttcc acgtgttcga
     8401 tctcgaaact tacacctttc tcagccagca caatgcggac ctgatggcta tagatgtcag
     8461 taggaccgga aaacagcgtc attaccgaac gtttgttggc agcgacagcc atgaaaacct
     8521 ccaggtatag tcagaatttt tactgctacc agccaccagg tggccagtca gaagttgtgt
     8581 tacccagtaa ggaacgactc tctttgttcg aaaatcaaac aaaaaatgag caatacccga
     8641 catttgggca gaaaattgga tgatagttta ccagattttg cgacctttgt ggtgagtcga
     8701 tgccggaaat ggggaaaaag agatgcgctt tagtctgaaa tagttgactt agtcccttat
     8761 tggcgatgtg gtttttgttt tacctgtctg tcaggtggca gcaaaaagca actttccagt
     8821 ttttacgctg attcagattt tagctataaa aaaacccgcc gaagcgggtt ttttcgaaaa
     8881 ttgttttctg ccggagcaga agccaattaa cgtttggaga actgcggacg acgacgtgct
     8941 ttacgcagac cgactttctt acgttcaacc tgacgagcgt cacgagtaac gaagccagct
     9001 ttacgcagtt cagaacgcag ggactcgtcg tattccatca gagcgcgggt gataccgtga
     9061 cggatcgcac cagcctgacc agagatacca ccacctttaa cggtgatgta caggtccagt
     9121 ttctcaacca tgtcgaccag ttccagcggc tgacgaacta ccatgcgggc agtttcacga
     9181 ccgaagtact gttccagaga acgttggttg attacgattt taccgttgcc cggtttgatg
     9241 aaaacgcgag ctgcggaact tttgcggcga ccagtgccgt agtattgatt ttcagccatt
     9301 gcctataatc ccgattagat gtcaagaact tgcggttgct gtgccgcgtg gttgtgctcg
     9361 ttacccgcgt aaactttcag tttacggaac atagcacgac ccagcgggcc ttttggcaac
     9421 atgcctttaa ccgcgatttc aatcacacgc tcaggacggc gagcaatcat ctcttcaaag
     9481 gtcgcttgtt tgataccacc gatgtggccg gtgtggtgat agtacacttt gtcagtacac
     9541 ttgttgccgg ttacagcaac tttgtcagcg ttcagaacga tgatgtaatc accggtatct
     9601 acgtgcggag tgtattccgc tttgtgctta ccgcgcaggc gacgagccag ttcagtagcc
     9661 agacggccca gagttttacc ggtcgcgtca acaacatacc agtcgcgttt tacggtttct
     9721 ggtttagctg taaaagtttt cattaaaagc ttacccaata aatagttaca cgttggtgaa
     9781 cacccaaacg tcttcaattg ttgaggttca cacgacaaag tccggcaaac ctaccccttc
     9841 gaatagccta tgccagcaca caaaaagttt tgggaaaaaa ctttcttgta acgtggggtc
     9901 gcaggattat agagaagtcg gggtcaaaga tcgacccctt tttgtgattt gtgacaggtt
     9961 ttaacccgcc aaatgctcgc gcttcagata ctcttcgctt tgcatctctt gcagacgtga
    10021 caggcaacgc tggaactcaa acttcagccg atcgccctga taaatttcat acagcggcac
    10081 ttctgcactc accactaatt tgacatggcg ctcgtaaaac tcatccacca gcgcaataaa
    10141 gcgccgcgct tcgctctcca tcaaccgcgt cataactggt acatcaaaca acatgaccgt
    10201 atgaaagagg cgtgagagcg caatatagtc atgctggctg cgggcatcga cacacaaggt
    10261 ggtaaaagag acagcaagcg tctgattctc agcgcccatt gtcgccagcg gtcgatggtt
    10321 gatttctaac gtcggtgaat tttcgcgttt cgctcccgcc agcgccaacc atagtttatc
    10381 catttgtgcc cgggtttcat cgtggagtgg cgaaagccac agatgcgcct gagtgagtgt
    10441 acgcagtcga taatcaacac cagcgtccac gttcattaca tcacaatgct gtttaatggc
    10501 atcgattgca ggcagaaaac gcgcacgttg caagccattt cgataaagtt cgtccggcgg
    10561 aatatttgac gttgctacca gggtaatacc gcgggcgaac agggctttca tcagaccgcc
    10621 aagtagcatg gcgtcggtaa tatcagaaac aaaaaattcg tcaaaacaga gcacgtcagt
    10681 ttcggcttta aagcgatcgg caataatttc cagcggatcg gtctgcccct gtaaggcagt
    10741 tagctcttcg tgcacccgca gcataaaacg gtgaaagtgc aggcgctgtt tccgctctcc
    10801 cggcaggctt tgatagaaaa ggtccatcag ccaggttttc ccgcgtccta caccgcccca
    10861 catatataag ccacgcactg gcgtatgctt tgtgtcttcg cgtttacccc acagcttacc
    10921 gacccgcgcc attagcccac tcgtcctggg ggctggtggc ctgctattga tgagttcttg
    10981 ataaataatt tccaggcggc tgacggcctc tttttgaacg tcgtcgggtt gatggctgcc
    11041 ttcattgagc gccttcaggt attgcgatgt tggggtaacg ctttgcatga tcttattatt
    11101 attccttgaa taatcggtgc gccgttgttc acggttgacg aaaaaaaggc cgttctacac
    11161 tacgcgatat gcagtcggga ttccacttct gtggaattaa cggttatagt ggcataatca
    11221 gccgtaggca tggagcctga agccaacacc ctacggaaac aaaagacaac gggagatgtt
    11281 catgacctgg gaatatgcgc taattgggtt agtcgtcggc atcattattg gtgctgtggc
    11341 catgcgtttt ggtaatcgta aactacgcca gcaacaggcg ttgcagtacg aactggaaaa
    11401 gaataaagct gaactggacg agtatcgcga agagctggtt agccactttg cccgcagcgc
    11461 ggaattactg gataccatgg cgcacgacta tcgccagctg tatcagcaca tggcgaaaag
    11521 ctccagcagc ctgctgccgg aactgtctgc tgaagcaaac ccgttccgta atcgtctggc
    11581 agagtctgaa gccagcaacg atcaggcacc ggtgcagatg cctcgcgact attctgaagg
    11641 tgcatccggc ctgctgcgta ctggcgcgaa gcgcgactaa tttatttttc gggcgcagcc
    11701 attgcgccct cctcttctct ccctccccga ctatcattta atctggtgtc tcattgttag
    11761 ccgtctgaaa attcaataac atcaaactgt tttgaatctc ttttcttatc attcaggtac
    11821 gagagcagga ataatgaaaa aacaaaccca gctgttgagt gcattagcgt taagtgtcgg
    11881 gttaactctc tcggcgtcat ttcaggccgt cgcgtcgatt ccaggccagg ttgccgatca
    11941 ggcccctctc cccagtctgg ctccaatgct ggaaaaagtg cttccggcag tggtgagcgt
    12001 acgggtggaa ggaacggcca gtcagggaca gaaaatcccg gaagaattca aaaagttttt
    12061 tggtgatgat ttaccggatc aacctgcaca acccttcgaa ggtttaggtt ccggtgtcat
    12121 catcaacgcc agtaaaggct atgtgctgac caacaaccat gtgattaatc aggcacagaa
    12181 aatcagtatt cagctcaatg atgggcgcga gtttgatgca aaactgattg gtagcgatga
    12241 ccagagcgat atcgccctgt tacaaattca aaacccgagc aaattaacgc aaatcgctat
    12301 tgccgactcc gataaattgc gcgtcggtga ttttgccgta gcggtcggta acccatttgg
    12361 ccttgggcaa accgccacct ctggcattgt ttccgcatta ggccgcagcg ggttgaatct
    12421 tgaaggtctg gaaaacttta tccagacaga tgcttccatt aaccgcggta actccggcgg
    12481 tgcactatta aaccttaacg gtgagttaat tggcatcaac actgcaatcc ttgcgcctgg
    12541 cggcgggagc gtcgggattg gatttgccat ccccagtaat atggcgcgaa cactggcgca
    12601 gcagcttatc gactttggtg aaatcaaacg cggtttgtta ggcatcaaag gcaccgagat
    12661 gagtgccgat atcgccaaag ccttcaacct tgacgtgcag cgtggcgcgt ttgtcagcga
    12721 agtgttgcca ggttctggct cggcaaaagc gggcgtcaaa gcgggcgata ttattaccag
    12781 cctcaacggc aaaccgctga atagctttgc tgagttgcgc tctcgtatcg cgactacaga
    12841 gccgggcacg aaagtgaagc ttggcctgct gcgtaacggc aaaccactgg aagtagaagt
    12901 gacgcttgat accagcacct cttcgtcggc cagcgctgaa atgatcacgc cagcgctgga
    12961 aggtgcaacg ttgagcgatg gtcagctaaa agattgcggc aaaggtatta agatcgatga
    13021 agttgtcaaa ggaagcccag ctgctcaggc aggcttgcaa aaagacgatg tgatcattgg
    13081 cgtcaaccgc gatcgggtga actcgattgc tgaaatgcgt aaagtgctgg cggcaaaacc
    13141 ggccatcatc gccctgcaaa ttgtacgcgg caatgaaagc atctatctgc tgatgcgtta
    13201 atgtcgtaaa ccgggcatca ggcttacgtg tgatgtccgg ttaactcgtg gtatgctgct
    13261 gccgttccct tttttaatga cgcctccatc atgtttgtga agctcttacg ttccgttgcg
    13321 attggattaa ttgtcggcgc tattctgctg gttgccatgc cttcgctgcg cagccttaac
    13381 ccgctttcca ctccgcaatt tgacagtacc gatgagacgc ctgccagcta taatctggcg
    13441 gttcgccgcg ccgcgccagc ggtggttaac gtttacaacc gtggtgtgaa caccaactct
    13501 cacaaccagc ttgagatccg caccctggga tccggtgtaa tcatggatca acgcggttat
    13561 atcatcacca ataaacacgt catcaacgac gccgatcaga tcatcgtcgc cttacaggat
    13621 ggacgtgtat ttgaagcatt gctggtggga tctgactctc taaccgatct ggcggtactt
    13681 aaaattaatg ccactggcgg tttacctacc attccaatta atgcacgtcg cgtaccgcac
    13741 attggcgacg tagtactggc gatcggtaac ccgtacaacc tcgggcagac cattacccaa
    13801 gggattatta gtgccacggg gcgaatcggt ctgaacccta ccgggcggca aaacttccta
    13861 caaaccgatg cttccattaa ccacggtaac tctggcggcg cgctggtgaa ctcgctggga
    13921 gaactgatgg gcattaacac gctgtcgttt gataagagta acgatggcga aacgccggaa
    13981 ggtatcggct ttgcgattcc tttccagtta gcaaccaaaa ttatggataa gctgatccgc
    14041 gatgggcgcg tgatccgcgg ctacattggc attggtggac gtgagatcgc accactgcac
    14101 gcgcagagcg gtggtataga tcaactgcaa gggatcgtgg ttaatgaagt gtcacctgac
    14161 ggcccggcgg cgaatgcggg tattcaggtc aacgatctga ttatttcggt ggataacaaa
    14221 ccggccatct ctgctctgga gacgatggat caggtggcag aaattcgccc tggttcggtg
    14281 atcccggttg tagtgatgcg tgatgataag cagttaacgc tgcaggtcac cattcaggaa
    14341 tatccggcaa ccaattaagt cgagtgctca aaacaaaaaa ccggagtctg tgctccggtt
    14401 ttttattatt cgctaatcaa ttacttatta acgaactctt cgcccagggc gatatctttc
    14461 ttgagcgtat ccagcatacc ttccagcgcg ttctgttcaa atgcgctcag ggtaccgata
    14521 gatttacgct cttccacgcc gtttttaccc agcagcagcg gttgagagaa gaaacgggcg
    14581 tactgaccgt cgccttcaac gtaggcacat tcgacaacgc cttgttcgcc ctgcagtgca
    14641 cgaaccagag acagaccaaa acgtgcagct gcctggccca tagacagggt tgcagacccg
    14701 ccaccggctt tcgcttcaac cacctcagta cccgcgttct ggatacgttt ggtcagatca
    14761 gccacttcct gctcggtaaa actaacgcca ggaacctgtg acagcagcgg cagaatggta
    14821 acaccagagt gaccgccaat aaccggcact tcaacttcgc ctggctgttt gcctttcagt
    14881 tccgcaacaa aggtgttgga acgaatgata tccagcgtgg taacgccgaa cagtttgttt
    14941 ttgtcataaa caccggcttt tttcagcact tcagcagcaa ttgcaactgt ggtgttaacc
    15001 gggttagtga taataccaat gcacgctttc gggcaggttt tcgcaacttg ctgtaccagg
    15061 tttttcacga tgccggcgtt aacgttaaac aggtcggaac gatccatacc cggtttacgc
    15121 gctacacctg cagagataag aacgacatct gcgccttcca gcgccggagt cgcatcttca
    15181 ccagaaaaac ctttgatttt cacagcagta gggatatggc tcagatcgac agccacaccg
    15241 ggagtcactg gagcgatatc atacagagag agttctgaac ctgaaggcag ttgggttttt
    15301 aacagtagtg caagcgcctg gccaataccg ccagcagcgc cgaggactgc gactttcatc
    15361 ctaaactcct tattatattg ataaactaag atatgttgct ccgctgccgc gaccttaatc
    15421 cacaaaattg ccgtttacaa tgaccacata tcaagaatgt gtagtcacgc aagtttagcg
    15481 tttatgcatt taattgccgt aatcaggaac ctaacttacg taattaacag tcgcttatgc
    15541 attagcgcaa cattccaaca ggtggtgaca atatacccta ccgttcagcc aaaacaacat
    15601 caatttgata acaattaatt tacttttaag cagaatttgc atgccgtgac gcaggcatgt
    15661 ttctcaataa cgaaatttga taaaatcccg ctctttcata acattatttc agccttcttc
    15721 agggttgact gtttgcataa aaattcatct gtatgcacaa taatgttgta tcaaccacca
    15781 tatcgggtga cttatgcgaa gctcggctaa gcaagaagaa ctagttaaag catttaaagc
    15841 attacttaaa gaagagaaat ttagctccca gggcgaaatc gtcgccgcgt tgcaggagca
    15901 aggctttgac aatattaatc agtctaaagt ctcgcggatg ttgaccaagt ttggtgctgt
    15961 acgtacacgc aatgccaaaa tggaaatggt ttactgcctg ccagctgaac tgggtgtacc
    16021 aaccacctcc agtccattga agaatctggt gctggatatc gactacaacg atgcagttgt
    16081 cgtgattcat accagccctg gcgcggcgca gttaattgct cgcctgctgg actcactggg
    16141 caaagcagaa ggtattctgg gcaccatcgc tggcgatgac accatcttta ccacccctgc
    16201 taacggtttc accgtcaaag acctgtacga agcgatttta gagctgttcg accaggagct
    16261 ttaatctctg ccccgtcgct tctggcggcg gggaaaatgt tgcttatccc tctcaacccc
    16321 ctgctttccc ctgcgattaa tttaacgaat agtgcagttt tactgcgaca tgtcattcac
    16381 ataatgaata catacggtaa aaaaagca
//
LOCUS       DAZPEB010000086        16404 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZPEB010000086 DAZPEB010000000
VERSION     DAZPEB010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16404
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.087"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..1224
                     /locus_tag="ACHURJ_002340"
     CDS             <1..1224
                     /locus_tag="ACHURJ_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031587700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISEcp1 family transposase"
                     /protein_id="HFR1281268.1"
                     /translation="SNAGLFLLLENAKSNGIFDFIENDLVFDNDSTNKIKMNHIKTML
                     CGHFIGIDKLERLKLLQNDPLVNEFDISVKEPETVSRFLGNFNFKTTQMFRDINFKVF
                     KKLLTKSKLTSITIDIDSSVINVEGHQEGASKGYNPKKLGNRCYNIQFAFCDELKAYV
                     TGFVRSGNTYTANGAAEMIKEIVANIKSDDLEILFRMDSGYFDEKIIETIESLGCKYL
                     IKAKSYSTLTSQATNSSIVFVKGEEGRETTELYTKLVKWEKDRRFVVSRVLKPEKERA
                     QLSLLEGSEYDYFFFVTNTTLLSEKVVIYYEKRGNAENYIKEAKYDMAVGHLLLKSFW
                     ANEAVFQMMMLSYNLFLLFKFDSLDSSEYRQQIKTFRLKYVFLAAKIIKTARYVIMKL
                     SENYPYKGVYEKCLV"
     gene            1480..2355
                     /locus_tag="ACHURJ_002341"
     CDS             1480..2355
                     /locus_tag="ACHURJ_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011091028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extended-spectrum class A beta-lactamase
                     CTX-M-15"
                     /protein_id="HFR1281269.1"
                     /translation="MVKKSLRQFTLMATATVTLLLGSVPLYAQTADVQQKLAELERQS
                     GGRLGVALINTADNSQILYRADERFAMCSTSKVMAAAAVLKKSESEPNLLNQRVEIKK
                     SDLVNYNPIAEKHVNGTMSLAELSAAALQYSDNVAMNKLIAHVGGPASVTAFARQLGD
                     ETFRLDRTEPTLNTAIPGDPRDTTSPRAMAQTLRNLTLGKALGDSQRAQLVTWMKGNT
                     TGAASIQAGLPASWVVGDKTGSGGYGTTNDIAVIWPKDRAPLILVTYFTQPQPKAESR
                     RDVLASAAKIVTDGL"
     gene            complement(2402..2746)
                     /locus_tag="ACHURJ_002342"
                     /pseudo
     CDS             complement(2402..2746)
                     /locus_tag="ACHURJ_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016943399.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
     gene            2760..5510
                     /locus_tag="ACHURJ_002343"
                     /pseudo
     CDS             2760..5510
                     /locus_tag="ACHURJ_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611787.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
     gene            complement(5586..6601)
                     /locus_tag="ACHURJ_002344"
                     /pseudo
     CDS             complement(5586..6601)
                     /locus_tag="ACHURJ_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            6996..7652
                     /locus_tag="ACHURJ_002345"
     CDS             6996..7652
                     /locus_tag="ACHURJ_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001516695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrS1"
                     /protein_id="HFR1281270.1"
                     /translation="METYNHTYRHHNFSHKDLSDLTFTACTFIRSDFRRANLRDTTFV
                     NCKFIEQGDIEGCHFDVADLRDASFQQCQLAMANFSNANCYGIEFRACDLKGANFSRT
                     NFAHQVSNRMYFCSAFISGCNLSYANMERVCLEKCELFENRWIGTNLAGASLKESDLS
                     RGVFSEDVWGQFSLQGANLCHAELDGLDPRKVDTSGIKIAAWQQELILEALGIVVYPD
                     "
     gene            complement(7971..8399)
                     /locus_tag="ACHURJ_002346"
                     /pseudo
     CDS             complement(7971..8399)
                     /locus_tag="ACHURJ_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003155741.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(8432..9823)
                     /locus_tag="ACHURJ_002347"
     CDS             complement(8432..9823)
                     /locus_tag="ACHURJ_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISKra4-like element ISKpn19 family transposase"
                     /protein_id="HFR1281271.1"
                     /translation="MQLTLQIVITDESGSSRTEELMTIQKSGETRNDIGLSVSESKLL
                     LNTVQQSVVQLQADEYTQHHIRCPHCLAARRIKGKQKIRYRTLFGVIPVSGLRVYRCR
                     CEESDTKTVSLLSDWAGDYSHPALKYIETRWASMISYEMTTRLLKDILPVGHSLNAST
                     VRNHLCQVAQRLDAEAEAHSGFLSGCPRDWGNLPRPGKPLVVGIDGGYVRDRDDKKRN
                     FEIIAGKSFSVGAPADTRRFGFVQKDDCHPERRLMTHLSAQGMQANQQIFFLSDGADN
                     LRDLQFGMYPESTHVLDWFHITMRLKVLMQYARGLLVSDPEAGSKVLALLESIKRYLW
                     HGNVVAALEHIDNCVMYCDDPELSYPSLKSLQKHLDEMYTYIRNNKMMIPNYGEMRRY
                     GEPVSTAFVESTINEVIARRMAKKQQMQWSRKGAHYLLQTRTAVLNNELQDKFVCWYP
                     GFQSDGKGPAMAA"
     gene            complement(9860..10432)
                     /locus_tag="ACHURJ_002348"
     CDS             complement(9860..10432)
                     /locus_tag="ACHURJ_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR1281272.1"
                     /translation="MQYAYIRVSSADQNTARQEEALSKAGFQPDKICVEHASAKDTNR
                     PGLQELLGQLRPGDTLLVHSIDRLCRNMSDMCAVTTRLRDQGVTLIFLKEQLTFSAGT
                     NNPMQELQLHMMSAFSQFERALLKERQADGIAAKKARGEKTGRPGADIKKIQEIDALR
                     SRGVRLRIACDHAGLGVSTYYKLRHQMNNQ"
     gene            complement(10569..11114)
                     /locus_tag="ACHURJ_002349"
     CDS             complement(10569..11114)
                     /locus_tag="ACHURJ_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HFR1281273.1"
                     /translation="MVWRRLRIAADTSLAALHFIFQIVQGWGDDHLHQFHIYGKDYGI
                     SYEGGIGFVDNPFRVVIDDFAFDAGDRFTYEYNFFEHWLHDIRVEAIYENSTLKAPFC
                     ISGHGMPGATAADEFDKTLAFLEAIVNADDETTVGEIRPFADDLDAVRFNRHKINRQL
                     SRLDLASPVLEPEVIWLGRRR"
     gene            11315..11719
                     /locus_tag="ACHURJ_002350"
                     /pseudo
     CDS             11315..11719
                     /locus_tag="ACHURJ_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(11826..12662)
                     /locus_tag="ACHURJ_002351"
     CDS             complement(11826..12662)
                     /locus_tag="ACHURJ_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HFR1281274.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(12662..13465)
                     /locus_tag="ACHURJ_002352"
                     /note="aph(3'')-Ib"
     CDS             complement(12662..13465)
                     /locus_tag="ACHURJ_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HFR1281275.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            complement(13526..14341)
                     /gene="sul2"
                     /locus_tag="ACHURJ_002353"
     CDS             complement(13526..14341)
                     /gene="sul2"
                     /locus_tag="ACHURJ_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HFR1281276.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            14671..14757
                     /locus_tag="ACHURJ_002354"
                     /pseudo
     CDS             14671..14757
                     /locus_tag="ACHURJ_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000954590.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
     gene            complement(15029..16033)
                     /locus_tag="ACHURJ_002355"
     CDS             complement(15029..16033)
                     /locus_tag="ACHURJ_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element IS5075 family transposase"
                     /protein_id="HFR1281277.1"
                     /translation="MENIALIGIDLGKNSFHIHCQDHRGKAVYRKKFTRPKLIEFLAT
                     CPATTIAMEACGGSHFMARKLEELGHFPKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTESQQAMRALHRVRESLVQDKVKTTNQMHAFLLEFGISVPRGAAVISR
                     LSTLLEDSSLPLYLSQLLLKLQQHYHYLVEQIKDLESQLKRKLDEDEVGQRLLSIPCV
                     GTLTASTISTEIGDGKQYASSRDFAAATGLVPRQYSTGGRTTLLGISKRGNKKIRTLL
                     VQCARVFIQKLEHQSGKLADWVRELLCRKSNFVVTCALANKLARIAWALTARQQTYEA
                     "
     gene            complement(16112..>16404)
                     /locus_tag="ACHURJ_002356"
     CDS             complement(16112..>16404)
                     /locus_tag="ACHURJ_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021138877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HFR1281278.1"
                     /translation="FFNRLGEIRDRSFEQQRYRASGLNLVTAAVVLWNTVYLERAAHA
                     LRGNGHAVDDALLQYLSPLGWEHINLTGDYLWRSSAKIGAGKFRPLRPLQPA"
BASE COUNT         4104 a         4176 c         4140 g         3984 t
ORIGIN      
        1 tcaaatgcag gtctttttct gctccttgag aatgcaaaaa gcaatgggat ttttgatttt
       61 attgaaaatg acctcgtatt tgataatgac tcaacaaata aaatcaagat gaatcatata
      121 aagaccatgc tctgcggtca cttcattggc attgataagt tagaacgtct aaagctactt
      181 caaaatgatc ccctcgtcaa cgagtttgat atttccgtaa aagaacctga aacagtgtca
      241 cggtttctag gaaacttcaa cttcaagaca acccaaatgt ttagagacat taattttaaa
      301 gtctttaaaa aactgctcac taaaagtaaa ttgacatcca ttacgattga tattgatagt
      361 agtgtaatta acgtagaagg tcatcaagaa ggtgcgtcaa aaggatataa tcctaagaaa
      421 ctgggaaacc gatgctacaa tatccaattt gcattttgcg acgaattaaa agcatatgtt
      481 accggatttg taagaagtgg caatacttac actgcaaacg gtgctgcgga aatgatcaaa
      541 gaaattgttg ctaacatcaa atcagacgat ttagaaattt tatttcgaat ggatagtggc
      601 tactttgatg aaaaaattat cgaaacgata gaatctcttg gatgcaaata tttaattaaa
      661 gccaaaagtt attctacact cacctcacaa gcaacgaatt catcaattgt attcgttaaa
      721 ggagaagaag gtagagaaac tacagaactg tatacaaaat tagttaaatg ggaaaaagac
      781 agaagatttg tcgtatctcg cgtactgaaa ccagaaaaag aaagagcaca attatcactt
      841 ttagaaggtt ccgaatacga ctactttttc tttgtaacaa atactacctt gctttctgaa
      901 aaagtagtta tatactatga aaagcgtggt aatgctgaaa actatatcaa agaagccaaa
      961 tacgacatgg cggtgggtca tctcttgcta aagtcatttt gggcgaatga agccgtgttt
     1021 caaatgatga tgctttcata taacctattt ttgttgttca agtttgattc cttggactct
     1081 tcagaataca gacagcaaat aaagaccttt cgtttgaagt atgtatttct tgcagcaaaa
     1141 ataatcaaaa ccgcaagata tgtaatcatg aagttgtcgg aaaactatcc gtacaaggga
     1201 gtgtatgaaa aatgtctggt ataataagaa tatcatcaat aaaattgagt gttgctctgt
     1261 ggataacttg cagagtttat taagtatcat tgcagcaaag atgaaatcaa tgatttatca
     1321 aaaatgattg aaaggtggtt gtaaataatg ttacaatgtg tgagaagcag tctaaattct
     1381 tcgtgaaata gtgatttttg aagctaataa aaaacacacg tggaatttag ggactattca
     1441 tgttgttgtt atttcgtatc ttccagaata aggaatccca tggttaaaaa atcactgcgc
     1501 cagttcacgc tgatggcgac ggcaaccgtc acgctgttgt taggaagtgt gccgctgtat
     1561 gcgcaaacgg cggacgtaca gcaaaaactt gccgaattag agcggcagtc gggaggcaga
     1621 ctgggtgtgg cattgattaa cacagcagat aattcgcaaa tactttatcg tgctgatgag
     1681 cgctttgcga tgtgcagcac cagtaaagtg atggccgcgg ccgcggtgct gaagaaaagt
     1741 gaaagcgaac cgaatctgtt aaatcagcga gttgagatca aaaaatctga ccttgttaac
     1801 tataatccga ttgcggaaaa gcacgtcaat gggacgatgt cactggctga gcttagcgcg
     1861 gccgcgctac agtacagcga taacgtggcg atgaataagc tgattgctca cgttggcggc
     1921 ccggctagcg tcaccgcgtt cgcccgacag ctgggagacg aaacgttccg tctcgaccgt
     1981 accgagccga cgttaaacac cgccattccg ggcgatccgc gtgataccac ttcacctcgg
     2041 gcaatggcgc aaactctgcg gaatctgacg ctgggtaaag cattgggcga cagccaacgg
     2101 gcgcagctgg tgacatggat gaaaggcaat accaccggtg cagcgagcat tcaggctgga
     2161 ctgcctgctt cctgggttgt gggggataaa accggcagcg gtggctatgg caccaccaac
     2221 gatatcgcgg tgatctggcc aaaagatcgt gcgccgctga ttctggtcac ttacttcacc
     2281 cagcctcaac ctaaggcaga aagccgtcgc gatgtattag cgtcggcggc taaaatcgtc
     2341 accgacggtt tgtaatagcg gaaacggaat ggggaaactc attccgtttt tgtttatcgc
     2401 cttagacggc aaaagtgctg tcgcccacct gcgcttgcgc ataccaggcc ataagctccg
     2461 tggttcctgg ttctccttcc gctggagccc agtgcgcata gtcatcggca gccacgggtt
     2521 gatagccacc gtgttttact tcaaaaatta tgccaccggt atccagcgac agcacggcat
     2581 gccaggttcc tgcggccatc tccagcaccg tacaggtttc ccccaatatc gcccgatggg
     2641 tgacggtacc ccgatcgtca aaattcagca ccacgaaacg accccttaat ggcaacagta
     2701 gctcgaaggt gtgagggtgt cggtgcgggc gcacgtaggt cccaggtcat attccttccg
     2761 gcgtccggca ttttaccgcc agacagctcg ggattcgtga tatcaccgtt cttgcagaat
     2821 acggtcagag ggaaaatacc cgccgtgagc atgcagcgct gatacgtcag cactatcagt
     2881 atcgtgaatt tgcctggccc tggacatttc gccttacccg tcttttatat acccggagct
     2941 ggataagcaa cgaacgtcct ggcctgcttt tcgatctggc gacagggtgg cttatgcaac
     3001 atcgtattat tctccccgga gccactacgc tgacccggtt gatttcagag gtaagggaaa
     3061 aggcgacgtt gcgcctgtgg aacaaactgg cactgatacc gtcagccgaa cagcgttcac
     3121 agctggagat gctgctgggg ccaactgatt gcagccgcct gtctttactg gaatcactga
     3181 aaaagggccc tgtgaccatc agtggtccgg cgtttaatga agcaattgaa cgctggaaaa
     3241 ctctgaacga ttttggcctg catgctgaaa acctgagtac actcccggct gtgcgcctga
     3301 aaaatctcgc acgttatgct ggtatgactt cggtgttcaa tattgccagg atgtcaccgc
     3361 agaaaaggat ggcggttctg gttgcctttg tccttgcatg ggaaacgctg gcgctggatg
     3421 atgcattgga cgttctggac gccatgctgg ccgttatcat ccgtgacgcc agaaagattg
     3481 ggcagaaaaa acggctccgc tcgctgaagg atctggataa atctgcattg gcgctcgcca
     3541 gcgcatgttc gtacctgctg aaagaagaaa caccggacga atcgattcgt gctgaggtgt
     3601 tcagctacat cccaaggcaa aagctggctg aaatcatcac gcttgtccgt gaaattgccc
     3661 ggccctcaga cgataatttt catgaagaaa tggtggagca gtacgggcgc gttcgtcgtt
     3721 tcctgcccca tctgctgaat accgttaaat tttcatccgc acctgccggg gttaccactc
     3781 tgaatgcctg tgactacctc agccgggagt tcagctcacg gcggcagttt tttgacgacg
     3841 caccaacgga aattatcagt cggtcatgga aacggctggt gattaacaag gaaaaacata
     3901 tcacccgcag gggatacacg ctctgctttc tcagtaaact gcaggatagt ctgaggcgga
     3961 gggatgtcta cgttaccggc agtaaccggt ggggagatcc tcgtgcaaga ttactacagg
     4021 gtgctgactg gcaggcaaac cggattaagg tttatcgttc tttggggcac ccgacagacc
     4081 cgcaggaagc aataaaatct ctgggtcatc agcttgatag tcgttacaga caggttgctg
     4141 cacgtctttg cgaaaatgag gctgtcgaac tcgatgtttc tggcccgaag ccccggttga
     4201 caatttctcc cctcgccagt cttgatgagc cggacagtct gaaacgactg agcaaaatga
     4261 tcagtgatct actccctccg gtggatttaa cggagttgct gctcgaaatt aacgcccata
     4321 ccggatttgc tgatgagttt ttccatgcta gtgaagccag tgccagagtt gatgatctgc
     4381 ccgtcagcat cagcgccgtg ctgatggctg aagcctgcaa tatcggtctg gaaccactga
     4441 tcagatcaaa tgttcctgca ctgacccgac accggctgaa ctggacaaaa gcgaactatc
     4501 tgcgggctga aactatcacc agcgctaatg ccagactggt tgattttcag gcaacgctgc
     4561 cactggcaca gatatggggt ggaggagaag tggcatctgc agatggaatg cgctttgtta
     4621 cgccagtcag aacaatcaat gccggaccga accgcaaata ctttggtaat aacagaggga
     4681 tcacctggta caactttgtg tccgatcagt attccggctt tcatggcatc gttataccgg
     4741 ggacgctgag ggactctatc tttgtgctgg aaggtcttct ggaacaggag accgggctga
     4801 atccaaccga aattatgacc gatacagcag gtgccagcga acttgtcttt ggccttttct
     4861 ggctgctggg ataccagttt tctccacgcc tggctgatgc cggtgcttcg gttttctggc
     4921 gaatggacca tgatgccgac tatggcgtgc tgaatgatat tgccagaggg caatcagatc
     4981 cccgaaaaat agtccttcag tgggacgaaa tgatccggac cgctggctcc ctgaagctgg
     5041 gcaaagtaca ggtttcagtg ctggtccgtt cattgctgaa aagtgaacgt ccttccggac
     5101 tgactcaggc aatcattgaa gtggggcgca tcaacaaaac gctgtatctg cttaattata
     5161 ttgatgatga agattaccgc cggcgcattc tgacccagct taatcgggga gaaagtcgcc
     5221 atgccgttgc cagagccatc tgtcacggtc aaaaaggtga gataagaaaa cgatataccg
     5281 acggtcagga agatcaactg ggcacactgg ggctggtcac taacgccgtc gtgttatgga
     5341 acactattta tatgcaggca gccctggatc atctccgggc gcagggtgaa acactgaatg
     5401 atgaagatat cgcacgcctc tccccgcttt gccacggaca tatcaatatg ctcggccatt
     5461 attccttcac gctggcagaa ctggtgacca aaggacatct gagaccatta aaagaggcgt
     5521 cagaggcaga aaacgttgct taacgtgagt tttcgttcca ctgagcgtca gacccctaaa
     5581 atcctgatgc tgatgtagtc ccgttttatc gtcatcacga agctttctgc tatgccattg
     5641 ctttccgggc ttcgcactgc tgtcgtacaa ggctccagtc ccagcaaccg ggcgaacgcc
     5701 ggcactgttg gcaactgcct tccgaagcgt ttttccaccg cacccagcat gacatcctgc
     5761 accgtttctt tgtcatagcc tccggtgctc gctgcccagt caatgatctc cctgtcgcag
     5821 cagtcctgcg cgaaggtcac ccgcagcttt tcgccattat cacagcggaa ctcaaagcca
     5881 tctgagcacc agcgccggtt actttccgct actgctacgc gccccttatg ggcccgctta
     5941 cggcaaggat cagcgggttt acgttcaaga agcagattat gcgttctcat aatgcgatac
     6001 acccgctttg cattaaccac aggcaggccg tcccgctccg actcccgtcg cagcagcgcc
     6061 cacacacggc gataaccata cgtcggcagg tcagccaccg ccatgtttat ccgggacaat
     6121 acggtggtat cgtcagaacg gggctgccgt ctgcgatcct gtcagtcaga tggccggtga
     6181 acccgaatgc tcaactgcgc acgcgacacg ccaagacttc tgcaaacgtc gcttattcgt
     6241 cgttcccagg caacgagggc gcatgcgcaa tccatttttt tgcccggccg aactccacgg
     6301 cctctttgag tatctcggct tccatcgtct ttttgcccag aaggcgctgg agttcgcgaa
     6361 tttgcttatt ggcggcagcg agttcggagg ccggcaccac ttcctcgccc gatgcaacag
     6421 ccgtcagtga accgtcttaa tattgcttgc gccatttgaa tatctggttt gcattgatgc
     6481 catgcaggcg cgcgacatga gacacggtca taccgggctc catagtctgc tgaataatgg
     6541 caattttttc ctgcggtgta cgacgccgac gccgctcagg tcctgataac acttcaacca
     6601 tcttatcgtt ctgactggta ttaaacatag ttccaagact acctcttatt ttaagagagt
     6661 cgaagtgtct ggtgatctat ggggccagtc tagccctctt ccaagtgtag ggagatatag
     6721 tcaaagtttg cttctccgcc gatgagtccg ccctgcgtcg gatagtccga ttttttaccg
     6781 gcgaagcacg gctctcctaa ccctatctaa agcgaataac ttttttcaac agggccgcgt
     6841 gccaatgcaa gagcgatagg cgctctacgc gccaatttga ccacttaaaa caggtaaatt
     6901 gaggggttgt aatgtgttga tgtaacaggc ttttatttta atgtcttggc atatgtataa
     6961 tggtagtcta gccctccttt caacaaggag tactcatgga aacctacaat catacatatc
     7021 ggcaccacaa cttttcacat aaagacttaa gtgatctcac cttcaccgct tgcacattca
     7081 ttcgcagcga ctttcgacgt gctaacttgc gtgatacgac attcgtcaac tgcaagttca
     7141 ttgaacaggg tgatatcgaa ggctgccact ttgatgtcgc agatcttcgt gatgcaagtt
     7201 tccaacaatg ccaacttgcg atggcaaact tcagtaatgc caattgctac ggtatagagt
     7261 tccgtgcgtg tgatttaaaa ggtgccaact tttcccgaac aaactttgcc catcaagtga
     7321 gtaatcgtat gtacttttgc tcagcattta tttctggatg taatctttcc tatgccaata
     7381 tggagagggt ttgtttagaa aaatgtgagt tgtttgaaaa tcgctggata ggaacgaacc
     7441 tagcgggtgc atcactgaaa gagtcagact taagtcgagg tgttttttcc gaagatgtct
     7501 gggggcaatt tagcctacag ggtgccaatt tatgccacgc cgaactcgac ggtttagatc
     7561 cccgcaaagt cgatacatca ggtatcaaaa ttgcagcctg gcagcaagaa ctgattctcg
     7621 aagcactggg tattgttgtt tatcctgact aattgctttg atgtgtgatt ttaaacgctc
     7681 aaatttataa aacgaataat attgtcaacc ttgcaataat agacataggg acaaccatgt
     7741 ataaattttt accctcaggc gtcttcacaa tagagccata tagcagtggg tcacttagtg
     7801 ggcttaattt ttccattaaa gataatatta atattgctca gtataaaaca tcatatggta
     7861 gcccatcttg gcaagtaaac ataaggctgc catttataag gcactgttgc aaaaatgtgg
     7921 aggtgatagg ggtactacgc cggaacccca gatttttccg tccgttcaaa ttatccacgg
     7981 ttgagcagct tgatcccgtc cgcccgcagt tgaccaacag gggaaatatg cctgtacaaa
     8041 gtttgccggg ttatcccaag ttcctggcat agcgtactga cctttgtttc tgattgtccc
     8101 attgatgcca ttgccagtcg cagtttgact ggcgtcattt tataaggtcg gccgccgttc
     8161 cgcccacggg ctcttgctga tgccagacct gcagtcgttc tttcagcaat cagttcgcgc
     8221 tcaaactccg ccagtgcggc aaagataccg aagacaagct tgccagcggc cgttgttgtg
     8281 tcgatagtcg ccccgtgacc ggtcagcact ttcagaccgg tccccctcgc ggtcaggtcg
     8341 tgcactgtat taatgagatg acgaagatca cgaccgaggc ggtccagctt ccatacgacc
     8401 gagagcataa aaaatattgg ggcagcgtga gctatgctgc catcgctggt cccttcccgt
     8461 cactttgaaa acccggatac cagcagacaa atttatcctg cagttcgtta ttcaaaacgg
     8521 cggtccgggt ctgtaacaaa taatgagccc cttttctgct ccactgcatc tgctgctttt
     8581 tggccattcg tctggcgatc acttcattga tcgtggattc cacaaacgcg gttgataccg
     8641 gctctccgta ccgacgcatt tcgccgtagt ttgggatcat catcttatta ttccggatgt
     8701 aggtatacat ttcatccaga tgtttttgca gggatttcag gctaggatag ctgagttcag
     8761 ggtcatcgca atacataacg caattatcaa tatgttccag cgcagcgaca acattgccat
     8821 gccagagata tcgtttaatg ctttccagca gtgccagaac tttactcccc gcctctggat
     8881 ctgataccag cagtccccgg gcatattgca tgagcacctt cagcctcatg gtgatatgaa
     8941 accagtccag cacatgcgtt gactcagggt acataccgaa ctggaggtcc ctgagattat
     9001 ccgcgccgtc ggacagaaaa aatatctgct ggttggcctg cattccctgc gctgaaaggt
     9061 gggtcatgag cctgcgttcc ggatgacagt catccttctg aacaaaaccg aagcggcggg
     9121 tgtcagcagg cgcaccaacg gaaaaagact tcccggcaat aatttcaaaa ttacgctttt
     9181 tgtcatcccg atcgcgaaca taaccaccat ctatacccac cacaagcggt tttcctggcc
     9241 tgggcaggtt cccccagtcg cgggggcagc cggaaagaaa acctgaatga gcttcagcct
     9301 cagcgtcaag acgctgcgcc acctggcata aatgattcct caccgttgag gcattcaggc
     9361 tgtggcccac cggcagaata tctttcagca gacgagtcgt catttcatag gagatcatcg
     9421 aggcccagcg ggtttcaata tatttcagtg ccgggtgaga ataatcgccg gcccagtcgc
     9481 tgagcaaact gactgtcttt gtatcactct cttcacaacg gcaccggtac acccgaagcc
     9541 cggataccgg aataacgcca aacagcgtcc ggtaccgtat tttctgtttg cctttgattc
     9601 tgcgcgcagc aaggcagtga gggcaccgga tatggtgttg agtatattcg tctgcctgca
     9661 gctggaccac cgactgctgg accgtattca gcaacagctt ggattctgac accgataatc
     9721 cgatgtcgtt ccgggtctcc cctgatttct ggattgtcat cagttcctcc gttcgactgg
     9781 agcctgattc atcggtgatg actatctgga gcgtcagttg catcattgcc tcctgctgtg
     9841 gtggatgact atctcccggc tactggttgt tcatctggtg acgaagctta taatatgtgg
     9901 aaacgccaag gcccgcatgg tcgcaggcga tcctgagcct gacccctctg cttctgagtg
     9961 cgtcaatttc ctggatcttt ttgatatcgg cgccggggcg ccctgttttt tcaccccgtg
    10021 cttttttcgc ggcgatgccg tcggcctgcc tttccttcag taaggctcgt tcaaactggc
    10081 tgaatgccga catcatgtgc agctgcagtt cctgcatcgg attgtttgtg ccggcgctga
    10141 acgtcagctg ctctttcaga aaaataaggg taacgccctg gtcacgaagc cgggtggtga
    10201 cagcgcacat atccgacata ttccggcaaa ggcgatcgat ggaatgaacc agaagcgtat
    10261 caccagggcg taattgcccc aggagttcct gtaaccccgg gcggtttgtg tccttcgcgc
    10321 tggcatgctc aacacaaatt ttatcaggct gaaaacccgc ctttgacaat gcctcttcct
    10381 gacgggccgt attctgatcg gccgagctta ccctgatgta ggcatattgc acgcaaaacc
    10441 tccggaaggt ctttaaacat catagagatt taaagaataa tccataaaca ctgatttagg
    10501 gaggtttata gacatttttt cggggatctg agaggcatgc tgaaaacgta tacctttata
    10561 gacatcagtc agcgacgacg gcccagccag ataacttcag gctccagtac cggagatgca
    10621 aggtcgagcc tgctcagttg ccggttgatt ttgtggcggt tgaacctgac agcatccagg
    10681 tcgtcagcaa aagggcggat ctcgccgacc gttgtttcat catccgcatt aacgatagct
    10741 tcaagaaacg ccagggtttt atcgaactca tccgcagctg tggctccggg cataccatgg
    10801 ccgcttatac aaaacggcgc tttcagcgta gagttttcat aaatggcctc aacacgaatg
    10861 tcatgaagcc agtgctcaaa gaaattgtat tcataggtaa agcgatcgcc agcatcaaaa
    10921 gcaaaatcat caatcacgac ccggaacgga ttatcgacaa aaccgatgcc tccttcgtag
    10981 gagataccgt aatctttgcc ataaatgtga aattgatgga gatggtcgtc cccccagccc
    11041 tgcactatct ggaaaatgaa gtgaagcgcg gccagtgacg tgtcggcagc aattctcagc
    11101 cgacgccaga ccatcggact gactccatga actgctattt tgatgacgta gatcttcata
    11161 gtcagcaggt attgttatga aatgatggtg gttattttaa cccctgtaga tggcatcatt
    11221 cgatggctgc cccgaggttt ttcatgctct cctaaaagaa ctttccgcta agcgatagac
    11281 tgtatgtaaa cacagtattg caaggacgcg gaacatgcct catgtggcgg ccaggacggc
    11341 cagccgggat cgggatactg gtcgttacca gagccaccga cccgagcaaa cccttctcta
    11401 tcagatcgtt gacgagtatt acccggcatt cgctgcgctt atggcagagc agggaaagga
    11461 attgccgggc tatgtgcaac gggaatttga agaatttctc caatgcgggc ggctggagca
    11521 tggctttcta cgggttcgct gcgagtcttg ccacgccgag cacctggtcg ctttcagctg
    11581 taagcgtcgc ggtttctgcc cgagctgtgg ggcgcggcgg atggccgaaa gtgccgcctt
    11641 gctggttgat gaagtactgc ctgaacaacc catgcgtcag tgggtgttga gcttcccgtt
    11701 tcagctgcgt ttcctgtttg gggtcgtttg cgggaagggg cggaatccta cgctaaggct
    11761 ttggccagcg atattctccg gtgagattga tgtgttccca ggggatagga gaagtcgctt
    11821 gatatctagt atgacgtctg tcgcacctgc ttgatcgcgg ccgcgatagc tagatcgcgt
    11881 tgctcctctt ctccatccgc gttccaagct gcggaaaggc acccataagc gtacgcctgg
    11941 tcgagcaggc gacgcggatc gacgtccagc gcacgagaga atgcgtccgc catctgtgca
    12001 atgcgtctag gatcgagaca aaggtcgtct ctgtcagccg gatcgtagaa catattggcg
    12061 gcgccaaagc ccacttcacc gaccagaccg acgggatcta tcaccagcca gccgcgactg
    12121 gagaacatga tgttttcatg atgcagatcg ccatgtagcc cacgcagttc cgaggcattg
    12181 ctcatcattt gatcggctat aatcgccgcg tggacgtagt cagtttgaca acctgcgttt
    12241 tgatcatcgc gcgcccgctg aaacaaagct gcaaagcgat cccggatcgg gagaagggca
    12301 gaaggcaggg gttcctcaga tgcggcatac agcttcgcca ttagttccgc tgcaatttcg
    12361 gtcgcctggt agtcgccgtg ctcggcaacg atgtgagaga gcattcgctc cccggcatat
    12421 tcgagcaaca tcagattgtt ctcacgaccg agcaaccgga ctgctcccct cccattgcgc
    12481 cataccagat agtcggcccc gcgcagttca tcagcaatgt cttctatagg tttcaatccc
    12541 ttgacgattg caggagtccc gtctggcaat gaaactttcc aaacgaggct ggaaaaggtg
    12601 tccgcaatga gaacaggttg cgaaacgtgc caatgagcag gaaaaacagg cggcatgaac
    12661 atcaacccca agtcagaggg tccaatcgca gatagaaggc aaggcgttcg cggtcggggg
    12721 cttcgatccc caatacattg aataggacag cgaaggcgcg ctctgcttca tctggcgctg
    12781 cccagttctc ttcggcgtta gcaatcatga gtgccaaatc ggcatagcga tctgctgttc
    12841 cgagccgccc aaggtcgatc agacccgtgc attgaagagt tttagggtcc accatgaagt
    12901 tcggcatgca gggatcacca tggcaaacaa ccatatcggt gcgctcttgg tcgagccgca
    12961 ccggtagctc tcgttcgaca cgagccaaaa gatcgagctg cggcgtactc ttgtcctcgt
    13021 ccggtaagaa gtcgggattg acggcattgc gggacaccac atcaacggcg cgtccgaaca
    13081 ttcgcgacag cctgcgctca aacggacatt gatcaaccga taggctgtga acagcgccaa
    13141 gttgctgccc cattgacggc cacgctttga gcaaatccgc tccagacaga tcagccgccg
    13201 gtactcccgg aattgccgtt atcaccaagc atgcaccctc ctgttcctcc tgccagttga
    13261 tgacctcggg gcaagccaca cctcgacctt tgagccaaat gaggcggtca cgctctccag
    13321 cgagctcacc gcggcgggaa gcaggtgcga ttttcgcgaa ggcatgcccg tcaccacgtc
    13381 gaaaaacaaa atcaccagat tctccgcctc tgacaggcaa ccagtcagaa tgcgattcac
    13441 caaaaaaaat attagttcga ttcaatggag gttccttcag ttttctgatg aagcgcgaat
    13501 atagagaaat atcccgaatg tgcagttaac gaattcttgc ggtttctttc agcgccgcca
    13561 ataccgccag cccgtcgcgc aaggggcgcg gctcgtgtgt gcggatgaag tcagctccac
    13621 ctgcggcggc ggcaagctct gcagcgagtg tcgcggcccc gacatccccc ggaccacggc
    13681 ctgtgagcgc gcgcagaaag gatttgcgcg aaacagacag aagcaccggc aaatcgaagc
    13741 gcagccgcaa ttcatcgaac cgcgccagca ccgagagcga ggtttcggga gcagccccca
    13801 gaaaaaaccc catgccggga tcaaggacaa ggcggttgcg tttgataccg gcacccgtca
    13861 gcgccgcgat gcgcgcgtca aagaacgccg caatgtgatc catgatgtcg ccagcgggtg
    13921 cctcgcgccg atctgcctgc ccgtcttgca ccgaatgcat aacgacgagt ttggcagatg
    13981 atttcgccaa ttgcggatag aacgcagcgt ctggaaaacc gcgaatatca ttgagatagg
    14041 ccacaccacg cgacaaggca taggcttgcg tcgcgggttg ataactgtcg agcgagacgg
    14101 gaatgccatc tgccttgagc gcgtccagca ccggcgcgat acgcgcgatt tctgtgtcgg
    14161 acgaaacagg cgcggcgtcg ggattgctgg atgccggacc gaggtcgatc acatctgccc
    14221 cctcggccat cagcttacgc gcctgcgcaa tggctgcgtc tggcgccaga taccggcctc
    14281 catcggagaa actgtccgag gttatgttga cgatgccgaa aatgatgagc gatttattca
    14341 tgggggcttc tataataata ataatcgagc atgagtctca tacggatgct cgggtcgaaa
    14401 gggaatcccc aggcgagtaa cctgtttgcg gtgctccatt agctgcagga gcagaatagc
    14461 atacatctgg aagcaaagcc aggaaagcgg cctatggagc tgtgcggcag cgctcagtag
    14521 gcaatttttc aaaatattgt taagcctttt ctgagcatgg tatttttcat ggtattacca
    14581 attagcagga aaataagcca ttgaatataa aagataaaaa tgtcttgttt acaatagagt
    14641 gggtatcgga gacgaagaag gacaaggtac atgctgaccg ataccaagct gcgcaatctc
    14701 aagcccaggg acaaactcta caaagtgaat gaccgggaag gtctctatgt gggggtcgcc
    14761 tcagaaaacg gaatctatgg tcactcccgt ttttgcaaca ccgattttga cgacaagttg
    14821 gcttgcttga atctatccgg cgtctgaatg ggattttatt cccgcgcctc gatgagttcc
    14881 gcgcctgatg aacctccaga aaatatacgg cttcaatgag cctttccgtt ttacaggttc
    14941 ctcaacaggc cggtgggccg ttagtatcat caatatcagt attcgcaaaa ccagatgaat
    15001 gattgtttaa actggtgtat ttctgccttt atgcttcgta agtttgctgt cgcgccgtca
    15061 gtgcccaggc tattctggcc agcttgtttg ccagagcaca ggtgacgaca aagttgcttt
    15121 tccgacacaa caactccctg acccagtcgg ccaacttgcc agactggtgt tccagttttt
    15181 gtatgaatac cctggcacac tgaaccaaca aagttcggat ctttttgttg ccccgcttgc
    15241 taatccctaa caatgtcgtc cgacctcccg tgctgtactg tcggggtacc agccctgttg
    15301 ccgccgcaaa gtcacggctg ctggcgtact gcttcccgtc gccaatctca gttgaaatag
    15361 tactggcagt cagcgttcca acgcagggaa tactcagcaa gcgctgtcca acctcatctt
    15421 cgtccaactt tcgtttcaac tgagattcca gatctttaat ctgctcaaca agatagtgat
    15481 aatgctgttg taatttcagc agtaactggc tgagataaag aggcaaacta ctgtcctcaa
    15541 gaagggtact cagtcgacta ataacggcag cacctcgcgg aacgctgata ccaaattcca
    15601 gcagaaaagc atgcatctga ttagttgttt tcaccttatc ctgaaccagg gattcacgga
    15661 cacgatgcag agctcgcatt gcctgctgag attcggttct gggctgcacg aaacgcatag
    15721 atggacgtga tgctgcttca cagatagctt cagcatcaac gaagtcattt ttgttgcttt
    15781 taacgaatgg gcggacaaat tgcggtgata tcagctttgg aaaatgccct aactcttcca
    15841 gcttgcgtgc cataaagtga gaaccgccac aggcttccat cgcgatggtt gttgccgggc
    15901 atgtcgccag aaattcgatt agctttggtc gggtgaattt tttacggtaa acggccttcc
    15961 cacgatgatc ctgacaatga atatggaaag agttcttacc cagatcgata ccaataagcg
    16021 caatgttttc catgatggtt ctccgaatga aagcctgtcc tcagcatagt actgggaagg
    16081 agggagtgac catctcatta aataaagcac gctaagccgg ttgcagcggt cgtagcggcc
    16141 tgaacttgcc cgcgccgatc ttggcgctgc tgcgccagag gtaatcgccg gtgaggttga
    16201 tgtgctccca accgagcggc gacaggtact gcaacagcgc gtcatcaacg gcatggccgt
    16261 tgccacgcag cgcgtgcgca gcccgttcca gatagaccgt gttccacaac acgacggcag
    16321 ccgttaccag attgaggccg ctagcccggt agcgctgctg ctcgaaactg cggtcgcgga
    16381 tttcacccag gcggttgaaa aaca
//
LOCUS       DAZPEB010000087        16383 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZPEB010000087 DAZPEB010000000
VERSION     DAZPEB010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16383
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.088"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..190
                     /locus_tag="ACHURJ_002357"
     CDS             <1..190
                     /locus_tag="ACHURJ_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1281279.1"
                     /translation="DMLNPTKDTNWNSTCIYKSRHKMLPVNLTQETLFNSKSQDKHAL
                     FPIFTASWRAYRIMNKGA"
     gene            413..910
                     /gene="ubiC"
                     /locus_tag="ACHURJ_002358"
     CDS             413..910
                     /gene="ubiC"
                     /locus_tag="ACHURJ_002358"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFR1281280.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            923..1795
                     /gene="ubiA"
                     /locus_tag="ACHURJ_002359"
     CDS             923..1795
                     /gene="ubiA"
                     /locus_tag="ACHURJ_002359"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFR1281281.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(1950..4373)
                     /gene="plsB"
                     /locus_tag="ACHURJ_002360"
     CDS             complement(1950..4373)
                     /gene="plsB"
                     /locus_tag="ACHURJ_002360"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFR1281282.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPSHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4544..4912
                     /gene="dgkA"
                     /locus_tag="ACHURJ_002361"
     CDS             4544..4912
                     /gene="dgkA"
                     /locus_tag="ACHURJ_002361"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFR1281283.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5022..5630
                     /gene="lexA"
                     /locus_tag="ACHURJ_002362"
     CDS             5022..5630
                     /gene="lexA"
                     /locus_tag="ACHURJ_002362"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFR1281284.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5703..7028
                     /gene="dinF"
                     /locus_tag="ACHURJ_002363"
     CDS             5703..7028
                     /gene="dinF"
                     /locus_tag="ACHURJ_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFR1281285.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHALWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7144..7353
                     /locus_tag="ACHURJ_002364"
     CDS             7144..7353
                     /locus_tag="ACHURJ_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFR1281286.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7395..7910)
                     /gene="zur"
                     /locus_tag="ACHURJ_002365"
     CDS             complement(7395..7910)
                     /gene="zur"
                     /locus_tag="ACHURJ_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFR1281287.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQSTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            8228..8482
                     /gene="yjbL"
                     /locus_tag="ACHURJ_002366"
     CDS             8228..8482
                     /gene="yjbL"
                     /locus_tag="ACHURJ_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjbL"
                     /protein_id="HFR1281288.1"
                     /translation="MLKIIPGATGYFNKTLNSNQFDNEDAIKDKLDNRGSIKGKLNNI
                     YGKSIDYAALRHRDIIIAKIDLFIQRITHNLWHARKKMCF"
     gene            8506..9213
                     /locus_tag="ACHURJ_002367"
     CDS             8506..9213
                     /locus_tag="ACHURJ_002367"
                     /inference="COORDINATES: protein motif:HMM:NF022352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFR1281289.1"
                     /translation="MWVNKYIDDCTDEDLNDRDFIASVVDRAIFHFAINSICNPGDNK
                     DATPIEQCTFDVETKNGLPSTVQLFYEESKDNEPLANIHFQTIGSGFLTFVNACQEHD
                     DNSLKLFASLLISLSYSSAYADLSETVYINENNESYLKAQFEKLSQRDMKKYLGEMKR
                     LADGGEMNFDGYLDKMSHLVNEGTLDPDILSKMRDAAPQLISFAKSFDPTSKEEIKIL
                     TDTSKLIYDLFGVKSEK"
     gene            9576..10613
                     /gene="dusA"
                     /locus_tag="ACHURJ_002368"
     CDS             9576..10613
                     /gene="dusA"
                     /locus_tag="ACHURJ_002368"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFR1281290.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECGMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDAYPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            10747..10989
                     /gene="pspG"
                     /locus_tag="ACHURJ_002369"
     CDS             10747..10989
                     /gene="pspG"
                     /locus_tag="ACHURJ_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFR1281291.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(11154..12137)
                     /locus_tag="ACHURJ_002370"
     CDS             complement(11154..12137)
                     /locus_tag="ACHURJ_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFR1281292.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGSGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            12220..13635
                     /gene="dnaB"
                     /locus_tag="ACHURJ_002371"
     CDS             12220..13635
                     /gene="dnaB"
                     /locus_tag="ACHURJ_002371"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFR1281293.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            13688..14767
                     /gene="alr"
                     /locus_tag="ACHURJ_002372"
     CDS             13688..14767
                     /gene="alr"
                     /locus_tag="ACHURJ_002372"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFR1281294.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAGAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            15020..16213
                     /gene="tyrB"
                     /locus_tag="ACHURJ_002373"
     CDS             15020..16213
                     /gene="tyrB"
                     /locus_tag="ACHURJ_002373"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFR1281295.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNLGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
BASE COUNT         3914 a         3905 c         4336 g         4228 t
ORIGIN      
        1 tgatatgttg aatcctacaa aagataccaa ttggaattct acgtgtatct ataaatccag
       61 acataagatg ttgcctgtta atcttactca ggaaacactt ttcaactcca aatctcagga
      121 taaacatgcg cttttcccca tttttactgc gagttggcga gcttatcgta taatgaataa
      181 gggtgcttaa gtaaaggaaa acatcaccgt tcctggcatc ctgggcggtg atgcccctat
      241 ggttgccctc gccagcgcgg gcatcggcaa agcgtaaggt tcaaaagcga tttaccactt
      301 catgcgattg ttgcgttttt gttgcgcatt agatcactta atttgcttta catctcccgt
      361 aaacactttt ctgcgataca atgcctttac gttatgtaac ggagagttcg gcatgtcaca
      421 ccccgcgtta acgcaactgc gtgcgctgcg ctattttaaa gagatccctg ccctggatcc
      481 gcaactgctc gactggctgt tgctggagga ttccatgaca aaacgttttg aacagcaggg
      541 aaaaacggta agcgtgacga tgatccgcga agggtttgtc gagcagaatg aaatccccga
      601 agaactgccg ctgctgccga aagagtctcg ttactggtta cgtgaaattt tgttatgtgc
      661 cgatggtgaa ccgtggcttg ccggtcgtac cgtcgttcct gtgtcaacgt taagcgggcc
      721 ggagctggcg ttacaaaaat tgggtaaaac gccgttagga cgctatctgt tcacatcatc
      781 gacattaacc cgggacttta ttgagatagg ccgtgatgcc gggctgtggg ggcgacgttc
      841 ccgcctgcga ttaagcggta aaccgctgtt gttaacagaa ctgtttttac cggcgtcacc
      901 gttgtactaa gaggaaaaaa atatggagtg gagtctgacg cagaataagc tgctggcgtt
      961 tcatcgctta atgcgtacgg ataagccaat tggcgcgtta ctgctgctct ggccaacatt
     1021 atgggcgttg tgggtggcga caccgggcgt tcctcagctc tggatcctgg cggtatttgt
     1081 cgcgggtgtc tggctgatgc gcgctgccgg atgtgtggtg aatgattatg ctgaccgaaa
     1141 gtttgatggt catgttaagc gcacggcgaa ccgaccactt cccagcggcg cggtaacaga
     1201 gaaagaggcg cgcgcgctgt ttgtagtgct ggtactgatt tcgtttttac tggtactgac
     1261 gctgaatacg atgaccattc tgttgtcgat tgccgctctg gcgttggcgt gggtgtaccc
     1321 gtttatgaag cggtataccc atctcccgca agtggtgctg ggcgcggcgt ttggctggtc
     1381 gattccaatg gcttttgccg ctgtgagtga gtcggtgcca ttgagttgct ggttaatgtt
     1441 cctcgccaat attctctggg cggtggctta cgacacgcag tatgcgatgg ttgaccgcga
     1501 tgatgatgtg aagattggca ttaaatccac ggcaatcctg ttcggccaat acgataaatt
     1561 gattattggt attttgcaga ttggcgtact ggcactgatg gcgatcatcg gtgagttaaa
     1621 tggcttaggc tggggatatt actggtcaat tctggtggct ggcgcgctgt ttgtttatca
     1681 acaaaaactg attgccaacc gcgagcgtga agcctgcttt aaagcattta tgaataataa
     1741 ctatgttggt ctggtactat ttttagggct ggcaatgagt tactggcatt tctgatgatg
     1801 taaaaaagcc ggatgatcat ccggctttct tctgggttgc ctgatgcgcg gcgcttctca
     1861 ggcctacaca acacatcgca atttattgaa tttgcagatt atggaatgcc ggataaggcg
     1921 ttttcgccgc atccggcaat tctctctggt tacccttcgc cctgcgtcgc actctcaatc
     1981 gtcaaacgca cgtctgatgt aatcaactcc gccagcaact gataaacctt catcgtttct
     2041 gccggttcgg catcgccgct atcgctgata tacccttcat cacgcagcgt cagcaccaga
     2101 gaactgaaca ccgccttgtc gaagaactcc ggcgcgttga taccgtgcag cacggagaga
     2161 cgttgcgcga cggtgcggct ctctttctcc agcgtaccgc ggttgatcga cgggttagcg
     2221 ctcaacagcc agaaggtgat ggcataacgt tgcagcgttt cgcgcgcgcc tgcggccagc
     2281 agctgtagcg tgcgagaatg cgacgggttg atatgcaact catcatcttg cagggtaatc
     2341 agcccctgac gttgcatctc atttgccaac gcatcaataa cgtccggcaa ctcgtcgcga
     2401 tcccagcgca ggaacagctc cgctttcagc attgggtaaa gcacattgac gtgctccatc
     2461 aatacgtcgc gggagatgtg gcgatgctgg gtgacaattg ccgccatcag cgaaggcagc
     2521 accaacatat gcgcaatgtt gttgcgatag taggtcatca gcaccgcttg ctcgcgcggc
     2581 agaatgatga tgtcgccgat agtgtctttc tcaacttcaa acttgttcat ttgcagcgcg
     2641 tgatcgataa gctcgctggc gctggctgaa ggaacggtag agtccgtgga gtaaggtacg
     2701 ttgcgcatca gatccaggta gcagttgagt tgctcggtta actgctcgcg ggtaagtgag
     2761 cgctgacgtg atgccagtag cgcggtacag cagaggttca tggcgtttgc cgcgcctgcg
     2821 ttgttaatgc gtaccatcag atcggcagca atattattga ccgtcggcgt taaccatgcc
     2881 ggacgcaccg cttcgatggg atcgatagat tcacgccagt caggtacatg ctggttaagg
     2941 taggtcatca acggcattgg ttcaccgaag ttgacgtaac cctgaccgag attacgcagc
     3001 ttgcttaaac cgcgcagcat ctgcggcagg ctctctttct ctttcgtcgc accgcgcagt
     3061 tctttggcgt aagtacccac ttccatgacg tgctcataac cgatatagat cggaatcagc
     3121 gtaatcggac gcgtgccgcc acgcagcatc gcctgaatgg tcatcgacag cgtaccggtt
     3181 ttcggatcca gcaaacgccc cgtacgggaa cgaccgcctt ccacgaaata ctcgacggaa
     3241 taaccacggc tgaacagttc gccgagatac tcacggaaaa cggtggaata aagtttattg
     3301 cctttaaacg tacggcgaat aaagaacgcc cccagacggc ggaaaatcgg cccggccggc
     3361 cagaaattca ggttgatccc ggcggcgata tgcggcggca ccagcccctg gtggtacagc
     3421 acgtaagaaa gtagcaggta gtccatgtga ctgcggtggc aaggcacata caccagctca
     3481 tgaccgtcgt gggccagctg gcgaacgcgc tcggcgttat ggacgttgat gccctgataa
     3541 agtcggttcc aggtgaagcc cagaatgcgg tcagtcaggc ggatcatctc gtaagagaaa
     3601 ttcgccgcaa tctcttccat cagcgcaatc gcgttctgct gcgctttttc atgggagatt
     3661 tttttgctgc gcgcttcatc ttctaccgct ttggcaatgg cgcgggaggc gagcagctta
     3721 ttaaacagat cctgacgagc aggcagacgt gggcccacag cagccagacg ttgacgggca
     3781 aagtgcatac gcgccacgcg cgccagtttc tgtgcgatag ttttatccgt gccgtgttca
     3841 tccgccatac ggcgcagcga aactgacggc gagaaacgca caaaactgtc gcgaccgagc
     3901 cacagtacgg cgaaaaattt ctgtacgccg ttaagcatac gcagcggcgg gtttacttcg
     3961 cctttttcac gccccggcgc gcgaccaaac atcaccgata ctggcaccat ctgcacatcc
     4021 agatttgggt tgctacggtg caaatcgaga tagtcgtgga acagcttaat agactcttct
     4081 ttcggcgtgt aataggtgaa tacacgcggc ccgccgtgaa tgaacacata gcgcggcagt
     4141 agcgtgccgt cgatttccag cggctctaac gggtcaggca agtcatgagc cagacactgg
     4201 gcgcgtaacg tcagcaaatc tgctttcgag ttgtacggta aaacgtacat aattggacga
     4261 gaggtatcca gccccagttc cggggcagga tctgccggaa tagacttgct ttttaccagg
     4321 atgcttaatg gtaaattcag taatttgtag taaattcgtg gccagccgga cataaacgat
     4381 gtaaagcctc tggttaataa tgcaaatgcg cggcaaggat agcagaaagt catgggaaat
     4441 tctgtggtat ccgctcatgt ttcgcgcggc gttacgcaaa cccgaatcat cggatttaac
     4501 ggtacactga tattgacgct cataatgtaa aaaggttctt tcaatggcca ataataccac
     4561 tggattcacc cgaattatca aagctgctgg ctattcctgg aaaggtttac gcgctgcatg
     4621 gatcaacgaa gcggcattcc gtcaggaagg cgtagcggta ttgttggcgg tggtcatcgc
     4681 ctgctggctg gatgtggacg caattacccg cgtgctgctt atcagctccg tgatgctggt
     4741 gatgattgtg gaaatcctca atagcgccat cgaagcagtg gttgaccgaa ttggctctga
     4801 ataccatgag ctttccggac gcgcaaaaga tatgggatcc gccgcggtgc tgattgcgat
     4861 tatcgtcgcc gtgataacct ggtgcatcct gttatggtcg cattttggat aacccttcca
     4921 gaattcgata aatctctggt ttattgtgca gtttatggtt ccaaaatcgc cttttgctgt
     4981 atatactcac agtataactg tatatacacc cagggggcgg aatgaaagcg ttaacggcca
     5041 ggcaacaaga ggtgtttgat ctcatccgtg atcacatcag ccagacaggt atgccgccga
     5101 cgcgtgcgga aatcgcgcag cgtttggggt tccgttcccc aaacgcggct gaagaacatc
     5161 tgaaggcgct ggcacgcaaa ggagttattg aaattgtttc cggcgcatca cgcgggattc
     5221 gtctgttgca ggaagaggaa gaagggttgc cgctggtagg tcgtgtggct gccggtgaac
     5281 cgcttctggc gcagcagcat attgaaggtc attatcaggt cgatccttcc ttattcaagc
     5341 cgaatgctga tttcctgctg cgcgtcagcg ggatgtcgat gaaagatatt ggcattatgg
     5401 atggcgactt gctggcagtg cataaaactc aggatgtacg taacggtcag gtcgttgtcg
     5461 cacgtattga tgacgaagtt accgttaagc gcctgaaaaa acagggcaat aaagtcgaac
     5521 tgttgccaga aaatagcgag tttaaaccaa ttgtcgttga ccttcgtcag cagagcttca
     5581 ccattgaagg gctggcggtt ggagttattc gcaacggcga ctggctgtaa catatctctg
     5641 agaccgcgat gccgccaggc gtcgcggttt gtttttcatc tctcttcatc aggcttgtct
     5701 gcatggcatt cctcacttca tctgataaag cactctggca tctcgcctta cccatgattt
     5761 tctccaatat caccgttccg ttgctgggac tggtcgatac ggcggtaatt ggtcatcttg
     5821 atagtccagt ttatttgggc ggcgtggcgg ttggtgcaac ggcgaccagc tttctcttta
     5881 tgctgttgct gtttttacgc atgagcacca ccgggctgac tgcgcaggct tatggtgcca
     5941 aaaatcctca ggcattagcc cgtgcgctgg tgcaaccgtt gctgttggcg ttgggggcag
     6001 gggcgttaat tgcgctgctg cgtacaccga ttatcgatct ggcactgcat attgttggcg
     6061 gtagtgaagc agtcctggaa caggcgcggc gctttcttga aatccgctgg ttaagcgcac
     6121 cggcgtcact ggcgaatctg gtattacttg gttggttact cggcgtgcaa tatgcccgtg
     6181 cgccagtaat tttgttagtg gtcggcaata tcctcaacat tgtgctggat gtctggctgg
     6241 tgatggggct gcatatgaac gtgcagggcg cggcgctggc gacggttatt gcggaatatg
     6301 caacattgct gattggtctg ctaatggtgc gtaaaatcct caaactacgc ggaatttccg
     6361 gcgaaatgct gaaaactgcc tggcgaggaa acttccgtcg cttgctggcg cttaaccgcg
     6421 atatcatgct gcgttcgctg ttgttgcaac tctgtttcgg cgcgatcacc gtacttggtg
     6481 cgcgactggg gagtgacatt atcgctgtta acgcggttct gatgacgcta ctcaccttta
     6541 ccgcctatgc gctggatggt tttgcctacg cggttgaagc gcattccggt caggcgtacg
     6601 gtgcgcgcga cggtagccag ttgctggatg tctggcgggc agcgtgccgc cagtcgggga
     6661 tcgtagcgtt actgttttcg gtagtttatt tgctggcagg ggaacacatc attgcgttgc
     6721 tgacgtcgtt aacccagatt cagcagctgg ccgaccgcta tcttatctgg caggtgattt
     6781 tgccgttggt tggcgtctgg tgttatctgc tggacggcat gtttataggc gcaacgcgcg
     6841 cagccgaaat gcgtaacagt atggcggtgg ccgccgcagg ttttgcgctg acgctcctta
     6901 cgctgccgtg gctgggtaat catgctttgt ggctggcgtt aaccgtcttt ctggcgttgc
     6961 gcgggctttc tctggcggct atctggcggc gtcactggcg caacggtacc tggtttgccg
     7021 caacgtgacg gttaaaaatt ctgaataaat aatcctaagc caaattgctg actacactta
     7081 atctcacgtt cagaagaaaa gtgaacgtac tctcattcac aacctaacga tgaggtcttg
     7141 attatgaata aagatgaagc cggcggtaac tggaaacagt ttaaaggtaa agtgaaagag
     7201 caatggggca aactgaccga tgatgatatg acgatcattg aaggtaaacg tgatcaactg
     7261 gtcggtaaaa tccaggaacg ttatggttat cagaaagatc aggcagaaaa agaggtcgtg
     7321 gattgggaaa cccgcaatga atatcgctgg taattaatcc ctcctgcccg acgtgtacaa
     7381 ggatgtacgc cctcttaacg cggtttcttt ttcacctgca cagagtgatc atgctggcac
     7441 tgttcaggat gacgacacgc ttccacttcc acgcatgccg cacagagccc atgtgcttca
     7501 atcacattat gccgcagggc aaaccccatt tttgccgcca gcgtatgcat gatgtcttcc
     7561 acgccttctg cacactcttc tttcactgcg ccgcagcgat cgcaaataaa catggctgaa
     7621 gtatgggtgg actggtcgaa cagatgacag agcacataac tgttggtgga ttccacctta
     7681 tgcacaaaac cttgctcaag cagaaaatcc agcgcgcgat aaaccgttgg cggcttggct
     7741 tgcggttcag cttcgcgcag taaatcaagc agatcataag cgctgatagc gccatcctgc
     7801 agactcatca ggcgcaacac ttccaggcgc tgtggggtca ggcgcacatt acgctgcgcg
     7861 cagatttttt cagcctgcgc taataactcc tgcgttgtgg tcttttccat cgggcacctc
     7921 aaagtggggg ttaagaatgt ccttacttta ccatgttcca ggaaaaacac cgagaaccct
     7981 tattgttgcc gtaatgttga ttttctgttt tgtaggtaag gtgttatgtt gccttgtcgt
     8041 accattatca acacgataat aattaataat tcatttttaa ataaattgct tcaagttaac
     8101 tcgctattta tttaactggg tttaaacgca tacctgtagt tcagaaatta tgagcgattt
     8161 ttgatagtga ccagatggca aaagaattat aaggaggaca cggtacgaca aaaaaggtag
     8221 atgaatcatg ttgaagataa tacccggtgc aactggatac tttaataaaa cactcaattc
     8281 aaatcaattc gataatgaag atgcaatcaa agataaatta gataatagag gttcaatcaa
     8341 aggtaaatta aataatatat atggtaaatc tattgattat gcagcgctgc gtcatcggga
     8401 tataattatt gcaaaaatag atttgtttat tcaacgaatc acgcataatt tatggcatgc
     8461 acgtaaaaaa atgtgttttt aattgagcaa ataaatgatc tcaaaatgtg ggtcaataag
     8521 tatattgacg attgcactga tgaggattta aacgatcgtg actttattgc atcagttgtt
     8581 gaccgggcta tttttcattt cgcgattaat agtatatgta atcctgggga taataaagat
     8641 gcgactccca ttgaacaatg tacttttgat gtagaaacta agaatggcct tccctccacg
     8701 gttcagctat tttatgagga atctaaggat aatgaacctt tagcgaatat acattttcaa
     8761 acaataggtt ctggtttttt aacgtttgtt aatgcctgcc aggaacatga tgacaacagc
     8821 ttaaaattat ttgcttcgct gttaatttca ctttcatatt ctagtgccta cgcagattta
     8881 tcagaaacag tgtatattaa tgaaaataat gagagctacc tgaaagctca gtttgaaaaa
     8941 ttatctcaac gtgatatgaa gaagtacctg ggagagatga agcgtctggc tgatggggga
     9001 gaaatgaatt ttgatggcta tctggataag atgtcacatc tggtgaatga agggacgctc
     9061 gatcctgata ttttaagcaa aatgcgagat gctgcaccac aattaattag cttcgcgaag
     9121 tcgtttgacc caacctcaaa ggaagagatt aaaatactta cagacacttc taaattaatt
     9181 tatgatttgt tcggggttaa atcggagaaa taatatgtga agttcttcga tagtatggaa
     9241 ggcattatat aaaaggaccc aatatttatt gggtcctttt ttctctatca atgctattag
     9301 cagggagata tatcaccaaa gtttaatgtg tgatttttta tttatcgtcg aacctggatt
     9361 gtttatcatt ggccttaaca aagttaacgg ctaataagat tatttccatc acttcgtgag
     9421 agcttcatgc cttgagagga tctcaatttt cttttgcaat gagacaggcg cttcctgttg
     9481 ttatggtata gtaccccgct attgagcctc ctgaatagtg atgctgaata acataacccc
     9541 atgatatatc gataaaataa tctctacatt tgaaaatgca cggtaattct gaaatgcaaa
     9601 aaatcaacca aaccagcgca atgcctgaaa aaactgacgt tcactggagt ggtcggttta
     9661 gcgttgcacc aatgctcgac tggacggaca gacattgccg ctatttcttg cgtctgcttt
     9721 cccgcaatac gttgctgtat accgaaatgg tgaccacagg ggcgattatt cacggtaaag
     9781 gtgattacct ggcgtacagt gaagaagaac atccggtagc gttgcaactc ggcggtagcg
     9841 atccggcggc gctggcacag tgtgcgaagc tggcagaagc gcgtggatat gatgagatca
     9901 acctgaatgt cggctgcccg tctgaccggg tgcagaacgg catgtttggt gcgtgtctga
     9961 tgggtaatgc gcagctggtt gccgattgcg tgaaagcgat gcgcgatgtg gtgtcgattc
    10021 cggtgacggt gaaaacgcgt attggcatcg atgaccagga cagctatgaa tttctctgcg
    10081 atttcatcaa caccgtttcc ggcaaaggcg agtgtgggat gttcatcatc catgcacgta
    10141 aagcctggct ttcggggtta agcccgaaag aaaaccgtga aatcccgccg ctcgattatc
    10201 cgcgtgtgta tcaactgaag cgtgactttc cgcatctgac aatgtcgatt aacggtggta
    10261 tcaagtcgct ggaagaggcc aaagcacacc tgcaacatat ggatggcgtg atggtcgggc
    10321 gcgaggcgta tcagaatccg ggtattctgg cggcggtaga ccgggagatc tttggttcct
    10381 cggataccga tgcctatccg gtggcggtag tgcgcgccat gtatccgtac attgagcgtg
    10441 aactcagcca ggggacgtat ctcggccata ttacccggca tatgttgggc ttgttccagg
    10501 gtattcctgg cgcgcggcag tggcggcgtt atttaagtga aaatgcccat aaagcgggtg
    10561 cagacattaa tgtgctggaa cacgcgctca aactggtggc ggataagcgt taacttttca
    10621 ccaaaaagta gtcaaattca ccacgccctg cgcaccgtcg cggggcgttt tgctgttaaa
    10681 tcaatagatt atttttggca tgattcttgt aatgccagca agagatttca tatttgggag
    10741 agcatcatgc tggaactact ttttgtgatt ggcttttttg tcatgctgat ggtcaccggc
    10801 gtttcgttgc tgggcattat cgccgcgctg gttgtggcga cggccattat gttcctcggc
    10861 ggtatgctgg cattgatgat taagttgctg ccgtggttac tactggcgat tgcggtggtg
    10921 tgggttatta aggcgattaa agcaccaaaa gtgcctaaat atcagcgtta tgaccgctgg
    10981 cgttactaag ggattgtgcg gatgatcaca acctaaggtt ttatccttag aacaaaatag
    11041 gaattgataa tcaaatctgt cactattgcg cctctaacag attcatcgtg ctgtacccta
    11101 catacagccg actataaaaa gaaagggctt cccaggtgga agccctattt cttttatgga
    11161 atcagcaggc tggaaccttg cgtcgcccgg ctttccagaa tctcatgcgc acgctgcgca
    11221 tccttcagcg gatatttctg ctgctcggcg acatcgacct taatcacacc gctggcaatc
    11281 aaagagaaca gttcattact ggcctcggtt aattcctccc gtgtggtgat atagccttgc
    11341 agggaagggc gtgtcacata caacgagcct ttttgattga gaatgcctaa gttcacaccg
    11401 gtaaccgcac ctgatgagtt gccaaaactg accattaagc cgcggcgttg caggcaatcc
    11461 agcgaccgtt cccaggtgtc tctgcccacg gaatcgtaca ctacgcgcac tttcttaccg
    11521 ccggtgatct ctttcaatcg ctcgaccaga ttctcttcac gatagttaat aacctgccac
    11581 gcgccggctt ttagcgcgct ctgcgctttt tgcgcggttc ctacggtgcc gataagtttc
    11641 gcgcccagag cttttgccca ctggcaggca atcaagccaa cgccgccagc cgctgcgtgg
    11701 aacaggaact gttcatcggg tttaatttca taggttttgc gcagcagata ataaaccgtt
    11761 aagcctttca ggaaagatgc tgcagcttgc tcaaaagaaa tagccgcagg cagaatcgcc
    11821 gctttatccg cattaatgtt atgcacagag ctgtaagcgc ctaacgccga ctgcgcatag
    11881 actacacgat cgcctgcctt aatatgcttt acaccactgc cgactttact cacgatgcct
    11941 gccgcttcgg tacctgatcc gctgggtagt gatggcggcg ggtaaaggcc gctgcggata
    12001 tacgtgtcga taaaattgat gccgatggct ttattttcga cctggatttc attctctgcc
    12061 ggatcggcag gagtgaactc tacggcttga agtacttccg ggccaccgtg cttgtgaaat
    12121 tcaattcgtg ttgccatgtg tcctccagaa cgtatcgtca gggtctgctt catatggtaa
    12181 agtttcgacc cattctttat ctcggtaact ccattcacta tggcaggaaa taaacccttc
    12241 aacaaacagc aggctgaacc ccgcgaacgc gatccacaag ttgccgggct gaaagtgcct
    12301 ccgcactcga tcgaagcgga gcagtcggtg ttgggcggtt taatgctgga taacgaacgc
    12361 tgggacgatg tagccgagcg tgtggtggca gacgattttt acacccgccc acaccgccat
    12421 atctttactg aaatggcgcg tttgcaggaa agcggtagtc ctatcgatct gattaccctt
    12481 gcggaatcgc tggaacgcca ggggcaactc gatagcgtcg gtggttttgc ttatctggca
    12541 gagctgtcaa aaaatacgcc aagtgcggcg aacatcagtg cttatgctga catcgtgcgt
    12601 gaacgtgccg ttgtccgtga gatgatctcg gttgcgaatg agattgctga agccggtttt
    12661 gatccgcagg ggcgtaccag cgaagatctg ctggaccttg ctgaatcccg cgtctttaaa
    12721 attgccgaaa gtcgtgcaaa caaagacgaa gggccgaaga acatcgccga tgtgctcgac
    12781 gcaaccgtgg cgcgtattga gcagttgttt cagcagccac acgatggcgt taccggagta
    12841 aacaccggtt atgacgatct caacaaaaaa accgctggct tgcagccgtc ggatttgatc
    12901 atcgtcgccg cgcgtccgtc gatgggtaaa acaacatttg cgatgaacct cgtcgaaaac
    12961 gcggcgatgt tgcaggataa accagtactt atcttctcgc tggagatgcc ttcagaacag
    13021 attatgatgc gttctctggc gtcgctgtcg cgcgttgacc agactaaaat ccgtaccggg
    13081 cagctcgatg atgaagactg ggcgcgcatt tccggcacca tgggtatttt gctcgaaaaa
    13141 cgcaatatct atatcgatga ctcctccggc ctgacgccaa cggaagtgcg ttcccgcgca
    13201 cgccgtattg cccgtgaaca cggcggcatc gggcttatca tgatcgacta cctgcaactg
    13261 atgcgcgtac cggcgctttc cgataaccgt acgctggaaa ttgcagaaat ctctcgctcg
    13321 ctgaaagcac tggcgaaaga actgaacgtg ccggtggtgg cgctgtccca gttgaaccgt
    13381 tctctggaac aacgtgccga caaacgcccg gtcaactccg acctgcgtga atctggctct
    13441 atcgagcagg atgcggactt gatcatgttt atctatcgtg atgaggtgta tcacgaaaac
    13501 agtgatttaa aaggcatcgc ggaaattatt atcggtaaac aacgtaacgg cccaatcggg
    13561 acggtacgcc tgacctttaa cggtcaatgg tcgcgcttcg acaactatgc ggggccgcag
    13621 tacgacgacg aataataatt attttatgaa ttaggcaatt aaagcaaaca cttatcaagg
    13681 aacacaaatg caagcggcaa ctgttgtgat taaccgccgc gctctgcgac acaacctgca
    13741 acgtcttcgt gaactggccc ctgccagtaa aatggttgcg gtggtgaaag cgaacgctta
    13801 tggtcacggt cttcttgaga ccgcgcgaac gctccccgat gctgacgcct ttggcgtagc
    13861 ccgtctcgaa gaagctctgc gactgcgtgc ggggggaatc accaaacctg tactgttact
    13921 cgaaggcttt tttgatgcca gagatctgcc gacgatttct gcgcaacatt ttcataccgc
    13981 cgtgcataac gaagaacagc tggctgcgct ggaagaggct agcctggacg agccggttac
    14041 cgtctggatg aaactcgata ccggtatgca ccgtctgggc gtaaggccgg aacaggctgg
    14101 ggcgttttat catcgcctga cacagtgcaa aaacgttcgt cagccggtga atatcgtcag
    14161 ccattttgcg cgcgcggatg aaccaaaatg tggcgcaacc gagaaacaac tcgctatctt
    14221 taataccttt tgcgaaggca aacctggtca acgttccatt gccgcgtcgg gtggcattct
    14281 gctgtggcca caatcgcatt ttgactgggt gcgcccgggc atcattcttt atggcgtctc
    14341 gccgctggaa gatcgctcca ccggtgccga ttttggctgt cagccagtga tgtcactaac
    14401 ctccagcctg attgccgtgc gtgagcataa agccggagag cctgttggtt atggtggaac
    14461 ctgggtaagc gaacgtgata cccgtcttgg cgtagtcgcg atgggctatg gcgatggtta
    14521 tccgcgcgcc gctccatccg gtacgccagt gctggtgaac ggtcgcgaag taccgattgt
    14581 cgggcgcgtg gcgatggata tgatctgcgt agacttaggt ccacaggcgc aggacaaagc
    14641 cggggatccg gtcattttat ggggcgaagg tttgcccgta gaacgtatcg ctgaaatgac
    14701 aaaagtgagc gcttacgaac ttattacgcg cctgacttca agggtcgcga tgaaatacgt
    14761 ggattaatcg ttctgtaata tttgattgtc tgtgccggat gcggcgtgaa tgccttatcc
    14821 ggccaataaa atcctaaaaa ttcaataagt tgatgttctt tcatgctctt ataaaggtcg
    14881 tgcctctggc ggatgttcgt ttgtcatgag tctcactctg ttgctaattg ccgttcgctc
    14941 ctgaacatcc actcgatctt cgccttcttc cggtttattg tgttttaacc acctgcccgt
    15001 aaacctggag aaccatcgcg tgtttcaaaa agttgacgcc tacgctggcg acccgattct
    15061 tacgcttatg gagcgtttta aagaagaccc tcgcagcgac aaagtgaatt taagtatcgg
    15121 tctgtactac aacgaagacg gaattattcc acaactgaaa gccgtggcgg aggcggaagc
    15181 gcgcctgaat gcgcagcccc atggcgcttc gctttattta ccgatggaag ggcttaacag
    15241 ctatcgccat gccattgcgc cgctgctgtt tggtgcggac catccggtac tgcaacaaca
    15301 gcgcgtagca accattcaaa cccttggcgg ctcaggggca ttgaaagtgg gcgcggattt
    15361 cctgaaacgc tacttcccgg aatcaggcgt ctgggtcagc gatcctacct gggaaaacca
    15421 cgtagcaata ttcgccgggg ctggattcga agtgagtact tacccctggt atgacgaagc
    15481 gactaacagc gtgcgcttta atgacctgtt ggcgacgctg aaaacattac ctgcccgcag
    15541 tattgtgttg ctgcatccat gttgccacaa cccaacaggt gccgatctca ctaatgatca
    15601 gtgggatgcg gtgattgaaa ttctcaaagc ccgcgagctt atcccattcc tcgatattgc
    15661 ctatcaagga tttagtgccg gtatggaaga ggatgcctac gctattcgcg ccattgccag
    15721 cgctggatta cccgctctgg tgagcaattc gttctcgaaa attttctccc tttacggcga
    15781 gcgcgtcggc ggactttctg ttctgtgtga agatgccgaa gccgctggcc gcgtactggg
    15841 gcaattgaaa gcaacagttc gccgcaacta ctccagcccg ccgaatttgg gtgcgcaggt
    15901 ggtggctgca gtgctgaatg acgaggcatt gaaagccagc tggctggcgg aagtagaaga
    15961 gatgcgtact cgcattctgg caatgcgtca ggaattggtg aaggtattaa gcacagagat
    16021 gccagaacgc aatttcgatt atctgcttaa tcagcgcggc atgttcagtt ataccggttt
    16081 aagtgccgct caggttgacc gactacgtga agaatttggt gtctatctca tcgccagcgg
    16141 tcgcatgtgt gtcgccgggt taaatacggc aaatgtacaa cgtgtggcaa aggcgtttgc
    16201 tgcggtgatg taatgcagga aagcaggctg gagttaccca gcctgcagtg aaattaaact
    16261 gtcgtcgctt tcactatttc tttgtagatg atttttttga tgccgttgtt ctacgtgaga
    16321 gataataaaa cgttgtcagt tcttttattg ttaagtttat cccaattaac tggaattcct
    16381 ttc
//
LOCUS       DAZPEB010000088        16083 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZPEB010000088 DAZPEB010000000
VERSION     DAZPEB010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16083
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.089"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(1..868)
                     /locus_tag="ACHURJ_002374"
                     /pseudo
     CDS             complement(1..868)
                     /locus_tag="ACHURJ_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
     gene            complement(843..1643)
                     /gene="otsB"
                     /locus_tag="ACHURJ_002375"
                     /pseudo
     CDS             complement(843..1643)
                     /gene="otsB"
                     /locus_tag="ACHURJ_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
     gene            complement(1810..2796)
                     /gene="araH"
                     /locus_tag="ACHURJ_002376"
     CDS             complement(1810..2796)
                     /gene="araH"
                     /locus_tag="ACHURJ_002376"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFR1281296.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(2811..4325)
                     /gene="araG"
                     /locus_tag="ACHURJ_002377"
     CDS             complement(2811..4325)
                     /gene="araG"
                     /locus_tag="ACHURJ_002377"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFR1281297.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(4395..5434)
                     /gene="araF"
                     /locus_tag="ACHURJ_002378"
                     /pseudo
     CDS             complement(4395..5434)
                     /gene="araF"
                     /locus_tag="ACHURJ_002378"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
     gene            6097..6600
                     /locus_tag="ACHURJ_002379"
     CDS             6097..6600
                     /locus_tag="ACHURJ_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFR1281298.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(6679..6930)
                     /gene="yecJ"
                     /locus_tag="ACHURJ_002380"
     CDS             complement(6679..6930)
                     /gene="yecJ"
                     /locus_tag="ACHURJ_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFR1281299.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFSNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(7045..7131)
                     /gene="azuC"
                     /locus_tag="ACHURJ_002381"
     CDS             complement(7045..7131)
                     /gene="azuC"
                     /locus_tag="ACHURJ_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFR1281300.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            7394..7717
                     /locus_tag="ACHURJ_002382"
     CDS             7394..7717
                     /locus_tag="ACHURJ_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFR1281301.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            7888..8385
                     /gene="ftnA"
                     /locus_tag="ACHURJ_002383"
     CDS             7888..8385
                     /gene="ftnA"
                     /locus_tag="ACHURJ_002383"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFR1281302.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQHLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(8423..8662)
                     /locus_tag="ACHURJ_002384"
     CDS             complement(8423..8662)
                     /locus_tag="ACHURJ_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFR1281303.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            8853..10064
                     /gene="tyrP"
                     /locus_tag="ACHURJ_002385"
     CDS             8853..10064
                     /gene="tyrP"
                     /locus_tag="ACHURJ_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFR1281304.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRRVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVR"
     gene            complement(10126..10791)
                     /gene="yecA"
                     /locus_tag="ACHURJ_002386"
     CDS             complement(10126..10791)
                     /gene="yecA"
                     /locus_tag="ACHURJ_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFR1281305.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTNHAILDVAELDDLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(10987..11073)
                     /locus_tag="ACHURJ_002387"
     tRNA            complement(10987..11073)
                     /locus_tag="ACHURJ_002387"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11037..11039),aa:Leu,seq:taa)
     gene            complement(11086..11159)
                     /locus_tag="ACHURJ_002388"
     tRNA            complement(11086..11159)
                     /locus_tag="ACHURJ_002388"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11125..11127),aa:Cys,seq:gca)
     gene            complement(11214..11289)
                     /locus_tag="ACHURJ_002389"
     tRNA            complement(11214..11289)
                     /locus_tag="ACHURJ_002389"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11254..11256),aa:Gly,seq:gcc)
     gene            complement(11441..11989)
                     /gene="pgsA"
                     /locus_tag="ACHURJ_002390"
     CDS             complement(11441..11989)
                     /gene="pgsA"
                     /locus_tag="ACHURJ_002390"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFR1281306.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(12046..13878)
                     /gene="uvrC"
                     /locus_tag="ACHURJ_002391"
     CDS             complement(12046..13878)
                     /gene="uvrC"
                     /locus_tag="ACHURJ_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFR1281307.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEDEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(13875..14531)
                     /gene="uvrY"
                     /locus_tag="ACHURJ_002392"
     CDS             complement(13875..14531)
                     /gene="uvrY"
                     /locus_tag="ACHURJ_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFR1281308.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            14673..14744
                     /locus_tag="ACHURJ_002393"
                     /pseudo
     CDS             14673..14744
                     /locus_tag="ACHURJ_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302039.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            14826..15002
                     /gene="yecU"
                     /locus_tag="ACHURJ_002394"
     CDS             14826..15002
                     /gene="yecU"
                     /locus_tag="ACHURJ_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFR1281309.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            14989..15213
                     /gene="yecF"
                     /locus_tag="ACHURJ_002395"
     CDS             14989..15213
                     /gene="yecF"
                     /locus_tag="ACHURJ_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFR1281310.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(15280..16028)
                     /gene="sdiA"
                     /locus_tag="ACHURJ_002396"
                     /pseudo
     CDS             complement(15280..16028)
                     /gene="sdiA"
                     /locus_tag="ACHURJ_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
BASE COUNT         4087 a         4099 c         3702 g         4195 t
ORIGIN      
        1 taacgaattt taccatgatg ctgcggatat ttttccagca acgcttcata ggcgagaaaa
       61 cgctctggca aacctttgga ataatccagc cgttcgacag aaaagatatt ttgtacgttt
      121 ttcagttccg ctttaagttg cgccagtttt ggcggcagtg gcccggcagc ctgtttggct
      181 atttctttcg gttcaatgcc gatcgggtag acttctgttc gaaatgcttt gccccaggct
      241 gtatggcttt ttgcgctacg tgtcgtgacg cgggtcaggt tagaaagaca atccaggaac
      301 gccagacgat cgttttctgt ctggaaaccc agcaaatcat aatcacaaag ctgttcaagc
      361 aaggtgtcat atgtcggcag cgcgttgaag atttccggtg tcgggaaagg aatatgcaga
      421 aagaaaccaa tgcgattatt caccccacgt ttgcgtaatt catgcgcaaa gggcaacagg
      481 tgataatcgt ggatccagat aatgtcatcg tcttgcaaca gcggcagtaa tttatctgcc
      541 agcaacgcat ttacgcgtag atagccgtcc caggcaggac gctgaaattg catcagatcg
      601 agccgataat gaaaagcggg ccagagaacg gcattggaga attggttgta gtattcgtca
      661 aggtcctgtt cgctgaggtt aaaagaggcc cacgtagtgt tacctttttt agcggctgat
      721 cctcattccc tgtttcacca ctccagccaa accacagtcc gcctgtggct ttcagtgccc
      781 ccagtatgcc aacggcaagg ccaccggcac tggcggcgtg ctcgtctggt ggtgcaatcc
      841 ggttagatac tacgactaaa cgactcatag tcatcactcc tgttattttc tcttttttgt
      901 tgtaatgcgg tggttatcat ttcaagcctg ctccagacat ccggcacacc cgccagtcgc
      961 catgatgcct gagttgcacc tgtgccaatt tttactgaca ttccgcccag tcggttaacg
     1021 actgcgaagc cagattcatc ggttaaatca tcgcccagaa atacgggcgt tcgcccgata
     1081 aagggagctt cctgcataaa agctgcaatt gcctcacctt tactggtacc tctcggtttg
     1141 atctcgacaa cacactttcc ctgctgtaac gccatttgtg gccagatctg agtaatacgt
     1201 tacgctaatg tcattaatgc gtcttcatgc tgcggagcct gacgataatg cagcgcaaaa
     1261 gccatccctt tcgcctccag ctccgcgccg ggatactgag cgataactgt atgcagttgc
     1321 acgctaatat cacgcgcaat cgcatccggc agatgaacga tatgcgtttt accattgatg
     1381 tcacggcgct ccgccccatg cacgcccgct aacgggaagc gataaggttt tgccagtgcg
     1441 tcaagctcca ccattgagcg ccctgatatc aatgccaatg caccatcact tgcggttgcc
     1501 agtagctgta gtccttgcag aatattgtca ggcacgacga cctgatcggg atgcggtttg
     1561 atttccgcca gcgttccatc gagatcaaaa aaccaggcat atttcgcgga tagttcaggg
     1621 gtttcggtta acggttctgt cacccggttc tcctacttct cttttcttat tgttttgcgc
     1681 agaacgcgct ttacagacat gtaagtatag acagtgtgac gggggtcgcc atttgcaacg
     1741 aagaacagcc aaccgttatg gcggttggct gtggtgggaa aaaacgctaa attgttgcag
     1801 aaaaaagcat cagacagtgc gtttcgcttt ttgcttgtaa cggtcgaaga tcaccgctgc
     1861 cagcaggatt aagccgcgaa ccacgtactg cgcgaaagga gaaatattaa gcaggttcat
     1921 ggcgttttcc acggtgccta aaattaagat acccgccacc acatatgaga tttttccgat
     1981 gcctcctttc agagaaacgc cacctaaaac gcaggcggag ataacaatca gctcgtaacc
     2041 aatcgacgtc attggctgcc cactggtcat acgtgaagcc agaataattc cggctatcgc
     2101 agataccagg ccagagagaa caaagataat aattttggtg cgaacaaccg gtacacccgc
     2161 cagacgcgca gcctcttcgt tcccgccaat cgccagggtg ttacgaccaa aggtggtttt
     2221 attcagcaac aaaccaaaga taatcagaca cgcgacggtg agccagattg gcgcaggcag
     2281 accgaaccag ttagcgtaac caagggcaaa gaagctttca tcttcgatac cgaccgcttt
     2341 accgtctgaa atgatgtacg ccagacctcg aacaatctgc atcgttgcca gtgttgtgat
     2401 cagagcattt attttcagtt tggcgataac aaagccattg accaggccac agagaatgcc
     2461 aagcagcaac cccgctgcca cgccaatcca caggctttca gtcaggttga taaccaccgc
     2521 cgtggtgaca cccgcacagg caattacgga ggcgacagaa aggtcaaagt caccggaagc
     2581 aaggcagaac aacatgccac acgccaccat ccccgacatg gaaattgcca ggcccaaccc
     2641 tttcatatta atgaaggtgg caaaatttgg gacaaaaatg gcacaggcga taaagagcac
     2701 cgcaaacacc accagcatgc cgtactgatc ccagatacgc ccgaagctga atgacgactt
     2761 aggtgcgcca gaacccgatg tagaaacaga agacatcata ctctccttac tcaggcaact
     2821 gcctggctga ctttaggcat cgcaaggctc agtgcctgac gctcatctgc ctgctcgtgt
     2881 aacaattcac cggcgatttc accttcccgc atcaccacaa tccggtcggc aacgccgagg
     2941 acttcaggta agtcgctgga ggcaaacagc accgccacac cctgcgccgc cagcgcataa
     3001 attacgttat atatttcgtg cttagcacca acatcaatgc cgcgcgtagg ttcatccagc
     3061 aaaatgacct tcatctcttc cgataaccag cggcccagaa tggctttttg ctgatttccg
     3121 cctgagagat tcatgatcag ttgctcagcg cccggcgttt tgatgttgag cgaacgaatg
     3181 tgctgatcgg cattgttttc ttcccaaccg ttgttgatta cacaaccgcc gagcacatgt
     3241 ttgcgtctgg cactgatgtt gatattgtcg cgaacggagt gcacgggaat aatgccttcc
     3301 gctttgcgat cttccgggca gagcatcatg cctgcggcaa tggcgtggct cggtttacga
     3361 atatcgatcg gctgttggtc gatataaacc tgaccggcgg tgatttgcgt cccgccaaac
     3421 aagcctttca ttaattcgct acgccccgcc cctaccagac caaacagccc aacaatttca
     3481 ccactgcgaa ccgccagact tattggcgta cgcacgcctg gtgctttcac agcatcaaga
     3541 cgcaggcgct cctcgccata actacgaggt tgccagccgt agatatcgcc aatgtcgcgc
     3601 ccgaccatcg cctgcaccag cgcgtcgtgg tcaacctgct gcatatcggt aaaggttttg
     3661 acataacgtc catctttaaa gacagtgatg gcatcgctga gggcaaatat ttcttccata
     3721 cggtgagaaa cgtataagat cacccgcccc tcttttcgca gttcacgaat aacgcggaaa
     3781 agattgtcga tttcgcgggc agaaagggag ctggttggct catcaaaggc gataatttta
     3841 gcgttacgcg ccagcgcctt ggcgatttca accatctgcc actgaccaat ggagagatat
     3901 ttcagcggcg tgtccgggtc aatatccata ccaagatgtt taagttgtaa acccgcctca
     3961 taattcagca atgagcgatt cacaatgccg cctttatgcg gcagctggcc gagatagatg
     4021 ttttccgcga cggtcatttc cggcacgaga tgcagttcct ggtaaataat cgccacaccc
     4081 gcgttaagtg ctgcggtcgt gtcggaaaag gacatttcct gcccattaat cactacagaa
     4141 cccgtggttg gcgcatagtt gccgctgagg atttttaaga gagttgattt tcctgcgcca
     4201 ttttcaccca tcaacgcatg aacctgaccg gcatagcagt caaaactaat atccgtcagc
     4261 gccttaacgc cgggaaacgt tttaccgatg ccgcgaaatg agagatacgg ggtagactgt
     4321 tgcataacgt ctccgtgaat cactggtcgt tcacaccccc tctgcatcat gcagagggga
     4381 atttttccgg caaattactt accgcctaaa ccttttttct ccagttcttc tttaaagttg
     4441 tcacgcgtga tcagcaccac gtcggtaact tcggtaaatt ttggcggttc aacgtctttt
     4501 gctacccagt tgtaaagcat ttcgctggat ttatagccat gtacgtccgg gcttggcagc
     4561 agggaaccgt agaagccggt tgcctgtact ttagacagtt cgctcaccgc atccacaccg
     4621 ttaatgccaa tgccgataat atcggcagct ttaaagccct gaccttccgt cgcgcgtacg
     4681 ccgcccagca cggtgctgtc gttcataccg acgatcagcc aatgtttaac ttcaggatgt
     4741 tgaaccagca ttgagttggc agcgtcaaat gcccccggga tgtcgttaga tttggtaggt
     4801 acctgataaa tttgtttttc cgggaatccg gccgctttca gcgcatccat agatcccgta
     4861 gtacggcggc gggcagtatc cagttcgttg gcggtaatca ccatcaccgc gctttctttg
     4921 acatcccagc cacgtttctg catctcttta tacagttcct gaccctgacg ttcgccaatt
     4981 ttagtcgcgc catcatcacc agcggaacgg tatccattgg cttacctttg gcgttaacaa
     5041 actgatcatc cacggcaatg actttcatat cgtagccacg cgctttcgcg acgatggcag
     5101 agccgagttt ggggtccgga gtacaaataa cgaaaccttt tgcgccactg gcagccaggc
     5161 tgtcgatcgc gttcaatgtt ttttcgccat ccggcacggc aatcttaata acctcaaacc
     5221 ctaaatcctt cccggcttta tcggcaaact tccattcggt ctggaaccac ggctcttccg
     5281 gttgcttcac cagaaaaccg agcttcaggt tctccgccat agcggattgt gacataacgg
     5341 ctgccagacc aatggctgcc agggctttag taaatttgtg catggttctc tccagcttta
     5401 gtgtcgtttt gtgtagggca aaaacgaatg acattcgtta aattaatcgg aaaacaaagc
     5461 attacctttt aactaaaaga taagtgaccg tgttgacata gttttagcga gaaattaatt
     5521 ctccatagga gagcaatatc acatcgcaga attacagtga gaacgtgcat aaatttagcg
     5581 ggaaaagaca taagggaaag ccaatttgtc agacaaattg tcgaatgcac agcagattaa
     5641 tccataagat tagcttggaa atccttgttg tctttggtac ccatgcggga tgtcttcttt
     5701 ttaaccagtc aataggccgc attacctggc gttgagtttt tgaaatggtg taataaccgc
     5761 aactcaaaga tgtggaaaat gcacgtcatt catttcgcca ttaattatca ctgtgctcat
     5821 taattaacag aacacgtata atgagagcca tctcgcaaaa atgaaaaaac gttttataaa
     5881 atcatcattt catcaatgaa atgtaaaaat atataaactt gatgatttaa gcattttctt
     5941 atacccgttc agacgttatt cttatttcag atcatcgtca gaattgactc cacgatcaca
     6001 tttcggaccg gcagaaagga attattctgc aaacagtaat tatggtgttt tgatttatct
     6061 tgcacctctc cacttctgga tataaggata ttaggtatgg caaccgctgg aatgcttctc
     6121 aaactcaact ctcaaatgaa ccgcgagttt tacgcatcca atctctacct tcacctgagt
     6181 aactggtgtt ctgaacagag tctgaacggc accgccactt tccttcgcgc ccaggcacag
     6241 agtaatgtga cccaaatgat gcgcatgttt aactttatga agagtgtcgg cgctaccccc
     6301 atcgttaaag ccattgatgt tcccggtgaa aaactgaact ctctggaaga actgttccaa
     6361 aaaacgatgg aagaatacga gcaacgttct agtacgttgg cacagttagc cgatgaagcg
     6421 aaagaactga atgatgattc aaccgtcaat ttcctgcgcg atctggaaaa agaacagcag
     6481 catgatggtc tgttgctgca aaccattctt gatgaagtgc gcagtgcgaa acttgctggt
     6541 atgtgcccag tgcagaccga ccaacatgtt ctgaatgtcg tgtcgcatca gctgcattga
     6601 tcatacatcg gcgctaatgc attgcgccga tgaagttttt gagaaaccgc tgcctcatct
     6661 ttttgaagca gcggtttttt aatgggattc gccttgtggg gtaaacttga gttcaatcag
     6721 cgcgatggct ttttggattg cccgcatggt gaccgggtct gcggcggcgg gatggttact
     6781 aaagtcgata ttcttcagct gactggacat tttttcacga acttcaacgg gcgcgattac
     6841 atcgagaaca tccagaattt gtttgataac caactggcaa gcaaccacat cagaaaccag
     6901 ttcctgatcg gcattcagcg gctgggacat cgtaaactcc tgatagcatt ttgaaagccg
     6961 ttatagtagc ggcttcacat cttcagcgat agtcacatcc accgtcatca ggacacaaaa
     7021 aaacctgccg gagcaggttt tttgttatcg gaacatattg cctggcggta cgtctttgaa
     7081 cgtcttgcaa tagttattga acatactttt caggattttg cgcagtttca tcgcggcact
     7141 ccgaccattt gttatacatg tgttattttc tttgtgctta taatatgaca accatcacaa
     7201 aaatcaatct ttatgtgatg caaatcacac aaataccccc ttaatgttat aaaaatgata
     7261 atcaaaaaac agccccccca tttctgacac ctacagatgg caagaaatag cgcctgccag
     7321 gcgtcttttc tggtcattgt cgcagcgctg taacgcgtaa aatagtgctt tcttttactc
     7381 ttctggctgg accatgagac ttctgattct gactctttca ttaataacgc tggcggggtg
     7441 tacggttact cgccaggccc atgtgagcga agttgacgcg gcaacaggta ttgtacggtt
     7501 ggtttatgac caggcttttt tgcagcatgc ccataccgat cgttatgtca gtcgcgggat
     7561 cgccgatcgc gcatgccagc aggaaggcta tacccacgcg atcccctttg gtcagccagt
     7621 aggcaactgt agcctttttg cgggttcctt atgtttgaat acggaattca ctttgtcata
     7681 tcagtgccat cattccgcct tccctgtctt tctctaaata ttttaatcag cgaggggatc
     7741 ttcgctgatt aaaaaaatag cggtaatgcg ttttattccc attcgtattt ttaataattg
     7801 aagtttatat tttacctttt gcaaataata aaataacaaa ttatagtgac gccacagcaa
     7861 caaatataac ctttgtggag cactatcatg ctgaaaccag aaatgattga aaaacttaat
     7921 gagcagatga acctggaact gtactcttca ctgctttatc agcaaatgag cgcctggtgc
     7981 agctatcata ccttcgaagg tgctgccgcg ttcctgcgcc gtcacgccca agaagagatg
     8041 acgcatatgc agcatctgtt tgattacctg actgataccg gcaatttacc gcgtattaat
     8101 accgttgaat ctccgtttgc tgaatattcc tcacttgatg aattattcca ggaaacctat
     8161 aaacacgaac aattaatcac ccagaaaatt aacgaactgg ctcatgctgc aatgaccaat
     8221 caggactacc caacatttaa tttcctgcaa tggtatgttt ctgagcagca tgaagaagag
     8281 aaactgttca aatcgattat tgataaatta agcctggcag gcaaaagcgg cgaaggtctg
     8341 tattttatcg acaaagaact ctctaccctc gacacacaaa actaatactc tccggcggca
     8401 ggtttactgc cgccgtttca aatcagtgac ggcaaatctt actctgatcg gtcgaaaaac
     8461 cgtctttcga aggtataaat ttgccttttg ctgccagaaa cgctaccagc tctcccgctg
     8521 tcatcccttc tgccgagcag gtgtgaaaac gtgcctgttc accaaaacgc gctttaattg
     8581 ctgcttccag actggcatgc gtatattgct cgcctgattc aatcatcata tttaacactt
     8641 catgaccgtg aatagagtcc atcgttcctc ctcaaaaaaa gcctagcgta gcgattgccg
     8701 cttatgaaga ctttgcgcca gagcagaact gaatgctttt tattgtacat ttatatttac
     8761 accatatgta acgtcggttt gacgaagcag ccgttatgcc ttaacctgcg ccgcagatat
     8821 cactcataaa gatcgtcagg acagaagaaa gcgtgaaaaa cagaactctg ggaagtgttt
     8881 ttatcgtggc gggaaccaca attggcgcag gcatgctggc aatgccgctg gctgcggcag
     8941 gtgttggttt tagcgtcacg ttaatcttgt tgattgggct ttgggcgttg atgtgctaca
     9001 cggcgctatt actgctggag gtgtaccagc atgttccggc agataccggt ctgggcacgc
     9061 tggcaaaacg ctatctggga cgctacggtc aatggctgac gggcttcagt atgatgttct
     9121 taatgtatgc tctgactgcg gcatacatca gcggtgccgg tgaattgttg gcctccagca
     9181 tcagcgactg gacaggtatt tcaatgtcgg caaccgctgg cgtgctgttg ttcacttttg
     9241 ttgccggtgg cgtggtttgt gttggaacat cgctggtcga tttatttaac cgttttctgt
     9301 tcagcgccaa aattattttt ctggtggtga tgctggtatt gctgctaccg catattcaca
     9361 aagtgaatct tttaaccctg ccgttgcaac aggggctggc tctgtcagca atcccggtga
     9421 tttttacctc atttggtttt cacggtagcg tgccgagtat tgtcagctat atggatggca
     9481 acgttcgtaa gctacgcagg gtgtttataa ccggtagtgc gatccccctg gtggcatata
     9541 ttttctggca ggtggcaacg cttggcagca ttgattcaac aacctttatg ggattgctgg
     9601 ctaatcatgc tggattaaac gggctgttac aggcgttacg cgaaatggtg gcctctccgc
     9661 atgttgagct ggcagtgcat ttatttgctg atttagccct cgccacgtca tttctcggcg
     9721 ttgcgttagg cttatttgat tatctggctg atttatttca gcgttcaaat accgttggtg
     9781 gacggttgca aactggtgca attacctttc tgccgccgtt ggcgtttgca ctgttttatc
     9841 cacgaggatt tgtgatggcg ctgggttacg ccggtgtggc gctggcggta ctggcattga
     9901 ttatcccttc gctgttgacc tggcaaagca gaaagcacaa tcctcaggcg ggttaccggg
     9961 tcaaaggtgg tcgtccggcg ctggtggtgg tgtttctctg tggtattgct gtgattggcg
    10021 tgcaattttt gattgcggca gggttgttac cagaagtgag gtgatcagat agcctcaaat
    10081 tccttattgg gtgccagaat taacgctgac acccaatttg gcctcttaat gcaggcagca
    10141 ctgcttaaat ttcttaccac taccgcacgg gcaaggatcg ttacgccccg gtttctcttc
    10201 tgctttgatc ggttgctgaa cagctttttc ctgcggatgc gccatccagt acgcatgtag
    10261 atcaagcgcc gccagtcgaa tggcatctac gctctcttca aacgcttctg gcgacatctt
    10321 ttctacccgc tcgaagtttt cctcagtacc gtgcagcgca atcgcctcca gcgctggttt
    10381 taacgaatcg ggcaacgttg accagtcaga aagtgccacg ccccgcatat agccaaagca
    10441 ccactcctca acaatcgtca gctcgctgcc atcaacttct cgcaagccga ataacggctc
    10501 aaactgctcc gggaattcgt tcagacgctc tgcggtatcg gccatatgtt gaaaagccag
    10561 gttcataaag cgcgtcatct ctttctctga cgcccagcgc ggcacatagt cagccccacc
    10621 ccacacggca accagccact gttccggttc aatctcttgc ggagaactca acaccgccgt
    10681 caataaatcg tccagctccg ccacatcaag gatggcgtgg ttagtgttgt atttggtcag
    10741 aatatcgtcc agccattcca actcactttc gtttaacggt cccgttttca tacgcttttc
    10801 cttgtggatc tcaactcgcc agcacctatc ttacatgccg gtccgtatca gagatacttt
    10861 ttgagtggct ttgccggtga ttaaaaatta aggagggtgt aacgacaagt tgcaggcaca
    10921 aaaaaaccac ccgaaggtgg tttcacgaca ctgcttattg ctttgatttt attcttatct
    10981 ttcccatggt acccggagcg ggacttgaac ccgcacagcg cgaacgccga gggattttaa
    11041 atcccttgtg tctaccgatt ccaccatccg ggctcgggaa gaaagtggag gcgcgttccg
    11101 gagtcgaacc ggactagacg gatttgcaat ccgctacata accgctttgt taacgcgcca
    11161 aattcttcag gcctttcagc cagacatccg cttgacgccg atgtctttta aactggagcg
    11221 ggaaacgaga ctcgaactcg cgaccccgac cttggcaagg tcgtgctcta ccaactgagc
    11281 tattcccgca ttcattaagc aatcagttaa tcacttgatt ttattatcgt ctggcaatca
    11341 gtgccgccgt tcgatgcgtt gcattctact tacctggcgc gatgagtcaa cgatattttt
    11401 caccactttt gatcgtttgc tgaaaattac gccgaaacga tcactgatca agcaaatctg
    11461 cacgcgcagc gctcaaatat tgcaacattg accacagagt cagcaccgca gccacaaaga
    11521 aaagtgcaat accggcgtac tcaacccaaa tgttcggacg ccacagcagc caggccaacg
    11581 ccaccatctg ggcagtggtt ttcactttcc caatccagga gacagccacg ctactgcgtt
    11641 tacccaactc cgccatccat tcgcgtagcg cagaaataat aatttcacgg gcgatcatcg
    11701 ttgccgccgg tagggtcacc caccagctgt gataatgctc ggttaccagc accatggcga
    11761 tagccacgag aactttatct gctacagggt caaggaaagc accaaaccgg gtactctggt
    11821 tccagcggcg tgccagaaaa ccatcgaacc agtcagtcac cgccgcgacg cagaaaatga
    11881 gcgcggcggc aaacggcgac caggtgacag gcagataaaa gaccaataca aagaatggga
    11941 taaggatgac acggaacagt gtaagcaacg tagggatatt aaattgcata atgacgggta
    12001 actatctgtt gtcagtaaga ttacccctat gttgctacag agacatcaat gtttcaacga
    12061 ccagaagatc ttttctgcca gaccttgcga aatacccggc acttttgcaa tttcctcgac
    12121 gctggcgtta cgtaaacctt gcaaaccgcc catatatttc aacaacattt gccgacgttt
    12181 tggcccgacg ccttcaatgg tttccaggga actggtattt ttgaccttcg cccgtttttt
    12241 acggtgcccg ccaatcgcgt gatcatgtga ttcatcgcga atatgctgga taacatgcag
    12301 cgcgggtgaa tctggcggca aactaaatcc ctcatcttcc ggctcaaaga acagcgtttc
    12361 cagtccagcc ttacgatctg ctcctttggc aacgccaagt agcagcggat gatttttatc
    12421 ccatgagaca tccagttcgg cgaagacatt tttcgcctgc gcaagctggc ctttgccgcc
    12481 gtcgataagg atcacatccg ggatcttact gtcgtcaatg gctttaccat aacgccgacg
    12541 cagcacctga ttcatcgccg cataatcatc gcccggcgtg atgcctgtaa tgttatagcg
    12601 ccgatactcc gcacgcagcg ggccgttagc atcaaacacc acacaggaag cgacggtttg
    12661 ttcgcccatg gtatggctga tgtcaaagca ctccatccgc ttcacttccg gcaatttcaa
    12721 cacgctggca agcgcggtca gtcgctggtg aacggtagat tgctgcgaaa gtttgctggt
    12781 taaggccgtc gccgcattgg tgcgcgcgag tttcagataa cgcgccctat cgccgcgagg
    12841 tttggtttga acattaatct tgcgtcccgc cagttctgaa agggaatcgg cgagcagcgt
    12901 tttatcgcta agattaaaat cgagcaggat ctcacccggt aaggtgcgca tctggctgcc
    12961 ttgtaaatag aactgaccta cgaaggtttc taccacctcg ctcagttccg taccgccagg
    13021 cactttcggg aaatagctgc ggctgccgag cactttgccc tgacgaatga acaatacgtg
    13081 gacacaagcc atgcccgcat cgaacgccac accaataacg tcaaggtcgt cgccggtatt
    13141 ggaaacaaac tgtttttcgg tgacgcgtcg caccgcctga atttggtcgc gaatacgtgc
    13201 cgcttcttca aactccagat tctgactggc agtttccata cggctaatga gttgcgtaag
    13261 cacctgatca tctttgccag acaaaaacag gcgcacatac tcgacctgct gagcgtattc
    13321 ttcttcactc accagtcctt caacgcacgg tcccagacag cgccctatct gatattgcag
    13381 acacggacgc gagcgattgc gataaacact attttcgcac tggcgaatgg ggaaaatctt
    13441 ttgcagtagc gccagagttt cacgtacggc atagccattc gggaacgggc cgaaatattc
    13501 acctttggca tgcttcgcac cacgatgcat cgccagacgc gggtgggtat cgccactcag
    13561 gaagataaaa ggataagatt tatcatcgcg tagcaaaacg ttgtaacgcg gctgatagag
    13621 tttgatgtag ttgtgttcca gcaacagcgc ttcggtttct gtatgagtaa ccgttacatc
    13681 aatttgctga atctgggcga ccagcgcttc ggttttgcgc gaagcgaggt tgctacggaa
    13741 atagctggaa agccgttttt tcaggtcttt cgctttgccg acatagataa ccgtaccacc
    13801 agcatcgtac atgcgataaa cgcctggctg gctggttacg gtttttaaaa acgcttttgc
    13861 gtcaaactga tcactcactg acttgataat gtctccgcat tacacagacc atggcgaatt
    13921 gccaggtgag tcagctcaac atcgccatga atgtttagtt tactgaacat acgatagcgg
    13981 tagctgttca ccgttttcgg actgagattg agctgttctg agatctcatt gaccttctgg
    14041 cccttggtga tcatcagcat aatctgcaat tcacgttcag acaaactggc aaatgggctt
    14101 tctgtttttt ctggttcgat ctggcttaac gccatttgtt gagcgatgtc agaagcaatg
    14161 taacgctgcc ctgaatagac agaacgaatc gcactcacga cttcctgcgg agccgcgcct
    14221 ttgctgaggt agcccgcagc accggcctgc atgactttcg ctggtaaagg gttttctgta
    14281 tgaacggtaa gcatgatgat tttgacatca gctgtggaac gcgcgatttt acgcgtcgcc
    14341 tcaagaccgc caatgcccgg catactcatg tccattagca ccacgtcaac ggcatttgtc
    14401 cggcaccact taacggcgtc ttcaccgcac gatgcctcac cgacgacttt tatacccttt
    14461 atatcttcca gaatgcgtcg tatccctgcg cgcaccagtt cgtggtcatc aacaagtaga
    14521 acgttgatca aagaaatatc tccagaaata gggataacgc tactgatagt tagtcattcg
    14581 tatattagcg gtttttattg caactttgaa acgttaaaaa tggtgggtat tcgatttttc
    14641 tctcgttttt gtcaattcga ttgtccacag ttatggtcac gcccgtcacc aacggcttac
    14701 cggatcgttt cacagtaagc cctacgatga aatgtgacaa aaattgactt tattcagcaa
    14761 aaatgaaaat cagcccgctg aatatgtaac attaattaac ccgaacaata cgggcaaaaa
    14821 cattcatgat aaaaatattt attggtcatt atattaacgt attttatagc actgccgata
    14881 tcacgctcaa aaaacaacca ctgctatttt tagcaaagct tatggtatac tccgccgcct
    14941 taacattttt caccgcaaat tttcattgca acatgacgag gaaaataaat gagtacgcct
    15001 gatttttcta ctgccgagaa taatcaagaa ctggcaaatg aagtctcctg cctgaaagcg
    15061 atgctgacgc tgatgctgca ggcgatggga caagctgacg cgggccgcgt gatgttaaag
    15121 atggaaaaac agcttgcgct gatcgaagac gaaacccagg ctgcagtatt ttccaaaacg
    15181 gttaagcaaa ttaaacaagc ctaccgtcag taatataaaa ccggctgaga gcgtgccttt
    15241 cagccggttt ttgcatctgg cacgcagaaa gaaaagcgat caaattaagc cagtagcggc
    15301 cgcgtaacag gccacctggg tcttatttgg tgcattgatt tttttctgca tgtttttctg
    15361 atggaaattg accgtattct cagagattga caaaatcatc gctatctctg ctgatgtctt
    15421 cccttccgcc gtccatttca gaatttcttt ttcgcgcttg ctgaaattca tctctggcgt
    15481 catcactatt tcatcattta aacgcatcag agccatcaga ctttcgcgca ccagtaactg
    15541 catttttaat tgcagttcat cactaagaat gggtatttcg cgcgtgctgc aacgggaaaa
    15601 ggacaaaaag cccagcgccc gatttggcag cattaaatac tgagtgacac cgcggcgtaa
    15661 accatgtgcg tgcgcggctt cccataatgg ctgtgcttcg ctgaataagt catcattcca
    15721 cattaaatgg ccctgactaa agttttcagg gttcagcaca ggatcaattg cgagaaaatt
    15781 agcacaggat caattgcgag aaaatttttt gcctgataat aactaaccca cgactcaggg
    15841 taattggtgt agaaagccac tttaggtcgc gtgaatggta ccgggtggcg aacacataac
    15901 gaatagtaat cgtactccag ctgctgagcc tgaagctcta tttcatggta gacctcttct
    15961 gcgacctcca tcctctgaaa acgcaacagc atcgtgcgac gccaactgaa aaaatccgta
    16021 tcctgcatag taaaccgcaa cgcccctgag agtgagtatc atttataatg ataattcaaa
    16081 cta
//
LOCUS       DAZPEB010000089        15959 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZPEB010000089 DAZPEB010000000
VERSION     DAZPEB010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15959
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.090"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            6..1241
                     /locus_tag="ACHURJ_002397"
     CDS             6..1241
                     /locus_tag="ACHURJ_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1281311.1"
                     /translation="MSKLPTGVEIRGKYIRIWFMFRGKRCRETLKGWEITNSNIKKAG
                     NLRALIVHEISSGEFEYLRRFPQSSTGAKMVTTRVIKTFGELCDIWTKIKETELTTNT
                     MKKTKSQLKTLRIIICESTPISHIRYSDILNYRNELLHGETLYLDNPRSNKKGRTVRT
                     VDSYIALLCSLLRFAYQSGFISTKPFEGVKKLQRNRIKPDPLSKTEFNALMESEKGQS
                     QNLWKFAVYSGLRHGELAALAWEDVDFEKGIVNVRRNLTILDMFGPPKTNAGIRTVTL
                     LQPALEALKEQYKLTGHHRKSEITFYHREYGRTEKQKLHFVFMPRVCNEKQKPYYSVS
                     SLGARWNAAVKRAGIRRRNPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYG
                     MWIDDMNDEQIAMLNARLS"
     gene            1293..2228
                     /gene="ttcA"
                     /locus_tag="ACHURJ_002398"
     CDS             1293..2228
                     /gene="ttcA"
                     /locus_tag="ACHURJ_002398"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFR1281312.1"
                     /translation="MQENQQITKKEQYSLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPVYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(2357..3730)
                     /gene="dbpA"
                     /locus_tag="ACHURJ_002399"
     CDS             complement(2357..3730)
                     /gene="dbpA"
                     /locus_tag="ACHURJ_002399"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFR1281313.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAVISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(3794..3901)
                     /gene="ynaL"
                     /locus_tag="ACHURJ_002400"
                     /pseudo
     CDS             complement(3794..3901)
                     /gene="ynaL"
                     /locus_tag="ACHURJ_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
     gene            complement(4176..5159)
                     /gene="zntB"
                     /locus_tag="ACHURJ_002401"
     CDS             complement(4176..5159)
                     /gene="zntB"
                     /locus_tag="ACHURJ_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFR1281314.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(5173..5220)
                     /gene="ydaN"
                     /locus_tag="ACHURJ_002402"
     CDS             complement(5173..5220)
                     /gene="ydaN"
                     /locus_tag="ACHURJ_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFR1281315.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            5414..6646
                     /gene="dgcM"
                     /locus_tag="ACHURJ_002403"
     CDS             5414..6646
                     /gene="dgcM"
                     /locus_tag="ACHURJ_002403"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFR1281316.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISSLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(6667..7230)
                     /gene="smrA"
                     /locus_tag="ACHURJ_002404"
     CDS             complement(6667..7230)
                     /gene="smrA"
                     /locus_tag="ACHURJ_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFR1281317.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIHQALADGLRNVLIIHGKGREDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(7560..8468)
                     /locus_tag="ACHURJ_002405"
     CDS             complement(7560..8468)
                     /locus_tag="ACHURJ_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1281318.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTPSLITQFRRECGYLQS"
     gene            8644..9944
                     /gene="abgA"
                     /locus_tag="ACHURJ_002406"
                     /pseudo
     CDS             8644..9944
                     /gene="abgA"
                     /locus_tag="ACHURJ_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
     gene            9944..11389
                     /gene="abgB"
                     /locus_tag="ACHURJ_002407"
     CDS             9944..11389
                     /gene="abgB"
                     /locus_tag="ACHURJ_002407"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HFR1281319.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGYIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSVTP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANFPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFTKQIQ
                     ATLPPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTLNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTLSSLK"
     gene            11426..12952
                     /gene="abgT"
                     /locus_tag="ACHURJ_002408"
                     /pseudo
     CDS             11426..12952
                     /gene="abgT"
                     /locus_tag="ACHURJ_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
     gene            12963..13478
                     /gene="ogt"
                     /locus_tag="ACHURJ_002409"
     CDS             12963..13478
                     /gene="ogt"
                     /locus_tag="ACHURJ_002409"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFR1281320.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLSDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNCTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            13673..14425
                     /gene="fnr"
                     /locus_tag="ACHURJ_002410"
     CDS             13673..14425
                     /gene="fnr"
                     /locus_tag="ACHURJ_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFR1281321.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            14577..15527
                     /gene="uspE"
                     /locus_tag="ACHURJ_002411"
     CDS             14577..15527
                     /gene="uspE"
                     /locus_tag="ACHURJ_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFR1281322.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFEPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(15577..15835)
                     /locus_tag="ACHURJ_002412"
                     /pseudo
     CDS             complement(15577..15835)
                     /locus_tag="ACHURJ_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
BASE COUNT         4137 a         3949 c         3911 g         3962 t
ORIGIN      
        1 atgatatgtc taaattacca acaggtgtcg agattcgagg taaatacatt cgcatctggt
       61 tcatgtttcg aggaaaacga tgtcgggaaa cattgaaagg ctgggagatt actaacagta
      121 acattaaaaa ggccgggaat ttaagagcgt tgatagttca tgaaatcagt tccggtgaat
      181 ttgagtattt aagacgtttt ccccagtcca gcactggggc aaaaatggtg acaacgaggg
      241 tcataaaaac attcggggag ctttgtgata tctggacaaa aattaaagag acagagttaa
      301 caacaaacac aatgaagaaa acgaaatcac aattaaaaac actcaggata ataatttgtg
      361 agagtacccc gatatcgcat attcgttata gcgatatctt aaactaccgg aatgaactgc
      421 tgcatggaga aacgctttac ctagataatc caagatccaa caaaaaagga agaaccgtgc
      481 gcacagttga tagctatatc gccctgctct gttcgttgtt acgttttgcg tatcagtcgg
      541 gatttatatc aaccaaacca tttgaaggag taaaaaaatt acagcgaaac agaataaaac
      601 ctgacccgtt atctaaaaca gaattcaatg cattaatgga aagtgaaaaa ggacagagcc
      661 aaaacttgtg gaaatttgcc gtttactccg ggcttcgtca cggggaactg gcagctctgg
      721 cgtgggagga tgtggatttc gagaagggaa ttgtgaatgt cagaagaaac ctgacgatac
      781 ttgatatgtt cggtccccca aaaacaaatg ccgggatccg gacggtaaca ttactgcagc
      841 cggctcttga agcactgaag gagcaataca aactgaccgg gcatcatcgc aaaagcgaaa
      901 tcacttttta tcatcgggag tacggcagaa ctgaaaagca aaaactgcat tttgttttca
      961 tgcccagggt gtgtaacgaa aaacagaaac cttattactc ggtaagcagt ttgggtgcaa
     1021 gatggaatgc agcagtaaaa cgtgctggta ttcgccgccg taatccgtac catacgcgac
     1081 atacttttgc ctgctggctg ttgacggcag gagcgaaccc ggcatttata gccagccaga
     1141 tggggcatga aactgcgcaa atggtgtatg aaatttacgg tatgtggatt gatgacatga
     1201 acgacgaaca gatagccatg ttgaatgcgc ggttatcgta gttgcaaagt ttgcccccaa
     1261 tttgccccat ttagtaccag agaactgaaa taatgcaaga aaatcaacaa attacaaaga
     1321 aagaacaata cagcctgaac aaattacaaa aacgtctacg tcgtaacgtg ggcgaagcca
     1381 ttgctgactt caatatgatt gaagaaggcg atcgcatcat ggtttgcctc tcagggggta
     1441 aagacagcta taccatgctg gagattctgc gcaatttgca gcaaagcgcg ccaatcaatt
     1501 tttcgctggt ggctgttaac ctcgatcaaa agcaaccggg cttcccagaa cacgttctgc
     1561 ccgtgtatct tgaaaagctg ggcgttgagt acaagattgt cgaagagaac acttacggta
     1621 tcgtgaaaga gaagattcca gagggcaaaa ccacttgctc actgtgttct cgccttcgtc
     1681 gcggtatcct ttatcgtacc gcaacggaac tgggggcgac gaagatcgcg ctgggtcacc
     1741 atcgtgacga tatcctgcag acgttgttct taaatatgtt ctacggcggc aagatgaaag
     1801 gtatgcctcc gaaactgatg agcgatgatg gcaaacatat cgttattcgt ccgctggcct
     1861 actgccgcga gaaagatatt cagcgatttg ccgatgcaaa agcgttcccg attattccgt
     1921 gcaacctgtg cggttcacag cctaacctgc aacgtcaggt gattgctgac atgttgcgtg
     1981 actgggataa acgttatcca gggcgtatcg agacgatgtt cagcgcgatg cagaatgtgg
     2041 tgccgtcgca tctgtgcgat accaacctgt tcgatttcaa aggcatcacc cacggttctg
     2101 aagtggttaa cgggggtgat ctggcgtttg atcgcgaaga gatcccacta caaccggcgg
     2161 gctggcagcc agaagaagat gaaaatcagt tggatgagtt acggctgaat gtggttgaag
     2221 tgaaataacc aggatagcgc ccgatgcgca agcgtatcgg gctactctta tggaggccgg
     2281 ataagacgcg gccaacgtcg catccggcaa tgccgaagaa gatgtttact cttgcacccg
     2341 gcaattcaac atttcattat tttaataacc gcacccggca cgtttttcct ttaatcttcc
     2401 cgccctgtaa ctgtttccat gctttatgag caacagcctg acggaccgcg acatagacat
     2461 gcgccggatg cacggcgatt ttgccaatat ctgcgccatc aagcccgata tctcctgtca
     2521 gtgcacctaa tacatcaccc gggcgcattt tggctttttt cccgccatcg atacacaacg
     2581 ttgccatttc tgcttccagc ggcacaatgg aactattagc tggcggcgtt tgccagttaa
     2641 gttttatctg caacatgtca gaaatgatat tggcccgctg tgcttcttcc ggagcacaga
     2701 aactgatcgc cagaccgcta tttcctgcac gagctgtacg gccgatgcga tgtacatgaa
     2761 cttcagggtc ccacgccagc tcaaagttca ccaccagctc aagcgattta atatccagac
     2821 cacgcgcagc aacatcagtc gcaaccagta cacgggcgct accgttagca aaacgtacca
     2881 gggtctgatc gcgatcgcgt tgctccaaat cgccgtgtaa tgacaatgca ctttgcccta
     2941 cttcattcag cgcgtcgcag acagcctggc aatctttttt ggtattgcaa aacaccacgc
     3001 aagaggatgg ctgatgcaag cttaataacc gttgcaacag aggaattttg cctttgctgg
     3061 atgtctcata aaattgttgt tcaatgggtg gcaaagcatc tgttgagtca atttcaatcg
     3121 ccaaaggatc gcgttgcact cgtccgctga ttacggcgat ggcttccggc caggttgccg
     3181 aaaacagaag cgtctgtcga gatgcaggcg caaaacggat gacatcatca atggcatcgc
     3241 taaatcccat atccagcatg cggtcggcct catccatcac caaagtattc agcgcatcca
     3301 gtgataccgt gcctttttgc aagtgatcca gcaaacgacc cggcgttgcc acgataatat
     3361 gcggcgcatg ttgcaacgaa tcacgctgca taccgaacgg ttgaccaccg cacaacgtca
     3421 aaattttggt atttggcaga aaacgcgcca gccgacgcaa ttcacctgcc acctgatccg
     3481 ccagttcacg cgtaggacac agcactaaag cctgggtttg aaatagcgac gcatcaattt
     3541 gctgtaacaa gccgaggcca aaagccgccg ttttgccgct gccggttttc gcctgcacgc
     3601 gaacatcttt tccggcaagg atcgccggaa gcgcggcggc ctgcaccggc gtcatagtta
     3661 aataacccaa ctcattaagg ttcgtgagtt gggcgggagg caaaacattc agggtagaaa
     3721 aagcggtcac aatctattct cgtggtcatc gacgcaaagt tagcaggcgc gtatcctcgc
     3781 agatctacgc gagtggtggg tcaggcatgg gttgcgggcg agggatcgga tcgggcactg
     3841 gaacaggatc gccaggaatc ggttcaggga caggaatttg caaataaata agtgtcgtca
     3901 tatttccctc cggtcattgg gtggactctt aaagggtaga cgctgataaa taacaggcaa
     3961 aaaaaagccg actcatcaaa gtcggcgtcg tacgaatcaa ttgtgctatg cagtaattca
     4021 aaaaaggaag taagacaata tggagcgcaa cgcccatcgc ttgacgttgc attcacctgc
     4081 aagggagata ttgccctgaa tgggtagaga gtttattgac ttcgctcaaa ctttgcggcg
     4141 tttttgtata cagacagccg gaaaaattgc ttttgttaca accatttact acgatgcaac
     4201 cataaagcaa caccaccaat aagaacaact aacagaatac aaaaaattga aaatccgaat
     4261 tgccacccgc cgccagggat cccaccaagg ttgacgccaa ataaccctgt cagaaaggta
     4321 ctgggtaaaa agaccattgc catcaatgac attgtatagg tacgacgagc taaattttcc
     4381 tgcatcacct gagcgatttc atccgccatc acgccagtcc gtgctataca ggcgtcgatt
     4441 tcgtcaagac cgcgcccaag gcgatcggca atatcctgca tccgacggcg ttggtcatcg
     4501 ctcatccacg gcaaacgttc actggcaaga cgagcataaa catcacgttg cggtgccata
     4561 tagcgacgca tcacgattaa ttgtttgcgc agcagagcca ggaatccacg cggtggaatt
     4621 tgctgatcaa ggagattatc ttcaaggtcg ataattttat cgtgcagctg ctcgataaat
     4681 tcgctggaat gatcggtcaa cgcatcgcac acatccacca gccaccctcc gcaatcggtc
     4741 ggacccgtgc cctcttccag atcgctcacc acatcgtcca gcgccagcac tttgcgttgt
     4801 cgggtcgaaa caattaaccg cccgtccata tatacacgca tggcgaccag ttgatcgggg
     4861 cgttcatcgg tgctgccgtt tatacagcgc aatgtaatca gcgtgccttc accgagacgg
     4921 ctgactcggg gccgtgtgct ctcgcctgcc agcgcatcac gtacgttatt gggaagcagc
     4981 ggtgttgtcg ccagccattg ggcgctatca tggtgtacat aattaaggtg gagccagcag
     5041 ggatgcgctt catcaatcac atctgtattt tccagcggtt taacgccgcc tctaccatcc
     5101 agcatccagg caaatactgc atccgggaca ttaacgtccg atcccttaat cgcttccaca
     5161 gtgcctccat catcaacgca ttattttgta gtctagcctt ctggccctgt tacgcaacat
     5221 ctcatcaccc cattaccctg aaatgattaa taaaattctg tctaaattga atacaaaaag
     5281 caaaatgctt ttccgtatac aaaccgtgtg aagtgttaaa tagcgtctat cattatcaga
     5341 attatctgat catatgacgt ggcttttttg cgatcggata gcaacaaaaa ttgataaaaa
     5401 taacgggatc tcaatgatta cgcacaactt caataccctg gacttactca ccagtcctgt
     5461 ctggattgtt tcgccctttg aggaacagtt aatttatgcc aatagcgcgg cgcgactgtt
     5521 gatgcaagac ctcacgttta gtcagctacg aaccggaccc tattccgtct cctcacaaaa
     5581 agaactgccg aaatacctct ccgatctgca aaaccaacac gatattatcg aaatcctcac
     5641 tgttcagcgt aaagaagagg aaacagcatt aagctgtcgg cttgttttgc gagagctgac
     5701 agaaacagaa ccggtgatta ttttcgaagg tatcgaagcg ccggcaacgc tgggtttaaa
     5761 agccagtcgc tcggcaaatt atcagcgcaa aaaacaaggt ttttatgcgc gcttttttct
     5821 gactaactct gcaccaatgt tgttgattga cccctcacga gatggccaaa tcgtcgatgc
     5881 taacctcgcc gcgctcaatt tctacggtta taaccatgaa acgatgtgcc aaaaacatac
     5941 ctgggaaatt aatatgctcg ggcgtcgcgt catgcctatc atgcatgaaa tttcgcattt
     6001 acccggcggt cataaacctt tgaattttgt tcataaactg gcggatggat ctactcgtca
     6061 tgtgcagacc tatgccgggc cgattgaaat ttatggcgac aagctcatgt tatgtattgt
     6121 gcatgatatt actgagcaaa aacggctgga ggagcagctg gaacatgctg ctcaccatga
     6181 cgcgatgacc ggattactga atcggcgaca gttttatcac attacggaac caggccaaat
     6241 gcagcacctc gccatcgccc aggattacag cttgttgctc atcgacaccg atcgttttaa
     6301 acacattaac gatctctatg ggcattctaa aggtgatgag gtgttatgcg ccctcgcccg
     6361 caccctcgaa agttgcgctc gcaaaggcga tttggtgttt cgttggggag gcgaagagtt
     6421 tgtcttattg ctaccaagaa ccccactgga taccgcgctt tcgctggctg aaactatccg
     6481 tgtaagcgtg gccaaagtga gtatttcgag cttaccacgt tttaccgtca gcattggtgt
     6541 ggcgcatcac gaaggaaatg aaagcatcga tgaactgttt aaacgcgttg atgatgcttt
     6601 gtatcgggcg aaaaatgatg gacgcaaccg cgtgctggcg gcataagccg cggatgcgtc
     6661 tcgagatcaa cggctgcgct tagcgtggcg ctcccagttt tcttgcttcg cctgcgccgt
     6721 tttacgtagt gcgacgtaac acgccccgct gccgccatga tgcggtagcg cggtgcaata
     6781 tgcctgaaca tcatcaaatt cggtcagcca gcgcgccaca tagctgcgga caatattggc
     6841 atgcgattta tcttcccgcc ctttaccatg aataatcagc acattacgca aaccatccgc
     6901 cagggcttgg tgaatgaaac tgaacaccat tttgcggcac tcttccaccg gctggcgcaa
     6961 aagattcagg ctcgcctgtt gcggatattt accactgcgc agcttatcca gcaccccatg
     7021 ttgcaaccct tcccgccgaa actccagcgg ctgacttagc gggatgatgt cgagaaatcc
     7081 ggtggtgagg aaattatcaa gctgcagcgt gtcgatacgc tgcggcgcac gttggttacg
     7141 cgttggatgc cagtggacat ctgtagcacg tttcagcggc tggacatctt ccatggcgtc
     7201 aagaaacagc gatttgtcgt caaggttcat gttacatcct cccgcaatta agagcgcgat
     7261 atgataacca gaccgggtcg gtccaacaac gtattaccaa aaattccagt aataagttcc
     7321 aaatattgcc gatattttaa gcaaaatgct tatgcatgat tattcattca cgatattaat
     7381 aatgtaactt atattttcgt gaaatctgtc actgaagaaa attggcaact aaaggttaaa
     7441 accgttataa cacagtcacc ggcgcagagg agacaatgcc ggacttaaga cgcggatgca
     7501 ctgctgtgtg tactgtagag tctggcggat gtcgacagac tctatttttt tatgcagttt
     7561 taactttgca gatagccgca ttctcgccgg aattgcgtga ttaatgacgg ggtcagtggt
     7621 gtctggcgac tatcacgccg ctgaatcaaa taataggccg ctttcggtaa aatttcgcta
     7681 accggcaaca tcaccagccc ctgtccgtgc aagggatcgc agcccatttc ttcaggcagt
     7741 atgctgagaa aatcgctttt tgccaccaga ctgatacagg ctgagaacgt ctcgcagact
     7801 acaccgacct gtggcgtttg cgcctgatcg tcaagcaatt cactcaactg tttgtagtaa
     7861 ctgccgtgtg gcgtcggcat tgtccagctg taatccagta actgtttgat cgaacgggca
     7921 ccaatggcgg ggtgtcccgg gcggcaaaag atcgcgaatt gcttttccag taatttctca
     7981 aaagtaaatt cgtggtcgta cggtccctga taataggtat tgatggtgaa atccaattct
     8041 ccctgacgca attcattaat catcgacacc agttgccctt ccataatgcg tacttttacc
     8101 tgcggatgct gctgatgaaa acgagatatg acagctggca tcagactgcg ggaaatactg
     8161 gcccccatgc cgatattaat ctgccctgcc agttgccctt gtcgttggcg aatatcctct
     8221 tgggctgcgc gcagctcttc aagaataaga ctggcgtgct gataaaaact ttcaccggca
     8281 tcagttaacg tcacgccttt actacggcga aaaaagagtt gcgccgctaa tccttcttcc
     8341 aactcctgaa tagatttact cagtgccggt tgcgacatat tcaacattcg gctcgctccg
     8401 cgaatgctgc cctgacgagc cacttcaaca aaagcacgaa tttgatgaat ttttacctga
     8461 aaagccatga cgccaccgat aaccgttatt tatcagacca aagaaactgg catctacttt
     8521 aatgcagatg attgtgtcag ggtaatttat gaacggttaa aactgtgaaa aatcagttag
     8581 tgataagtaa aaactatcgc tacgtgaacc gggtcacact ttttactgat gacgggaaag
     8641 gttatggagt ctttgaatca atttgttaat tcgcttgccc caaaattatc gcactggcga
     8701 cgtgattttc atcactatgc agagtctggc tgggtggaat tccgcactgc cacccttgtt
     8761 gcggaagaat tgcagcagct cggctattca ctggcgctgg gccgcgaagt agttaatgaa
     8821 agtagccgga tgggactacc tgatgaactc actctacaac gcgaattcga gcgcgctcgt
     8881 caacagggtg cgctagcaca atggattgcg gtttttgaag gtggtttcac tggtatcgtc
     8941 gccaccctgg ataccggtcg ccccggtccg gtgatggctt tccgtgtcga tatggacgcg
     9001 ctggatctca gtgaagagca ggatgtcagc catcgcccct accgcgacgg ttttgcgtca
     9061 tgtaacgccg gaatgatgca tgcctgtggt catgatggac ataccgccat tgggcttggg
     9121 ctagcgcata cccttaaaca attcgagtcc ggactacatg gcgtcatcaa actgattttt
     9181 cagcctgcag aggaaggtac gcgtggcgcg cgggcgatgg tcgatgcagg tgtcgtagat
     9241 gatgttgatt attttactgc cgtgcacatt ggcactggcg tacctgcggg caccgtggtg
     9301 tgcggcagtg ataattttat ggcaaccacc aaatttgacg cgcacttcac cggtaccgcc
     9361 gctcacgcag gcgcaaaacc agaagacggt cacaatgcct tgttggcggc agcacaagcc
     9421 actcttgcac tgcatgcaat cgccccggag cttccagagt aaacgtgggc gttatgcagg
     9481 caggaagcgg tcgtaacgtt gttcctgcct cggcgttgct gaaagtggaa acacgcgggg
     9541 ccagcgacgt cattaatcaa tatgtttttg accgtgcaca acaagcgatt cagggcgcag
     9601 caaccatgta tggtgtcggc gttgaaactc gtctgatggg tgcagctacc gccagttctc
     9661 cttcgccgca atgggtcgca tggttgcaaa gtcaggcggc tcaggtcgcg gggggcaatc
     9721 aggccattga acgtgttgaa gcgcctgcgg gttccgaaga tgccacatta atgatggccc
     9781 gcgtgcagca acatcaaggg caagcctcct acgtggtgtt tggcacacag ctggcggcag
     9841 gtcatcacaa cgaaaaattc gattttgacg agcaggttct cgctattgcc gtcgaaacgc
     9901 tggcgcgcac cgcgctcaat tttccctgga cgcgaggtat ctgatgcagg aaatctatcg
     9961 ttttatcgac gatgcgattg aagccgatcg ccaacgttat accgatattg ccgatcaaat
    10021 ctgggatcat ccagaaacac gttttgaaga gttctggtca gcggagcatc tggcttcggc
    10081 gctggaatct gcaggcttca ccgttacccg caacgtaggc tatatcccaa atgcctttat
    10141 tgcttcgttt ggtcaaggca aaccggttat cgccctgctg ggagaatatg acgccctggc
    10201 aggtttaagt cagcaagcag gttgcgcgca acctacatcc gtgacgcccg gtgaaaatgg
    10261 tcacggttgc ggacacaatt tgctgggaac cgccgccttt gccgctgcaa tagccgtcaa
    10321 gaaatggctg gaacaatatg ggcaaggcgg cacggtgcgc ttttatggtt gtcctggcga
    10381 agaaggcggc tcgggtaaaa cgttcatggt tcgcgagggg gtatttgatg atgtggatgc
    10441 ggcactcact tggcacccgg aagcctttgc cggtatgttc aatacccgca cgctggcaaa
    10501 cattcaggca tcatggcgct ttaaagggat cgcagcacat gccgcgaatt tccctcattt
    10561 gggacgcagc gcccttgatg ccgtaacgtt gatgaccact ggcaccaact tcctcaacga
    10621 acatattatt gaaaaagcgc gcgtacacta tgccatcaca aatagcggcg ggatctcgcc
    10681 caacgtggtc caggcgcagg cagaagtgct ttatcttatc cgcgcccccg aaatgaccga
    10741 cgtgcagcat atttatgatc gggtcgccaa aatcgccgaa ggtgcggcat tgatgaccga
    10801 aaccacggtt gaatgccgct tcgacaaagc ctgttccagt tatctcccga atcgcacctt
    10861 agaaaatgcc atgtaccatg ccctatccca ttttggtacc ccggaatgga actccgaaga
    10921 actggctttt acgaaacaaa ttcaggctac gctccccccc aacgatcggc aaaacagtct
    10981 gaataatatc gccgcaaccg gtggcgaaaa cggcaaggct tttgcactac gtcatcgtga
    11041 aacggtactg gcgaatgagg tcgctccata tgccgccacc gataacgtgc ttgcggcatc
    11101 gactgatgtc ggcgacgtca gttggaaact gcctgttgcc cagtgtttca gcccctgttt
    11161 tgccgtcggt acaccgctac atacgtggca actggttagc caggggcgaa catctattgc
    11221 tcataaagga atgctgctgg cggcgaaaac tatggcagca accacactca atctcttcat
    11281 tgattcaggg ctattgcaag aatgccaaca agagcatcag caagttacgg acacgcaacc
    11341 gtatcactgc cctatcccaa aaaacgtgac actgtcatct ttaaaataac aacaacaacg
    11401 caaacacaac aaccgaggaa tgcccatgag tatgtcatcc ataccgtcgt cctcctaatc
    11461 cgggaagctc tatggctggg tcgaaagaat tggtaacaag gttccccatc cttttttgct
    11521 ctttatctat ttgattatcg tactcatggt gacgacggca attttgtcgg cctttggcgt
    11581 cagtgcgaaa aacccgaccg atggaacgcc ggtggtggtg aaaaacctgc tcagtgtgga
    11641 aggattacac tggtttttac ccaatgttat taaaaacttt agcggttttg ctccacttgg
    11701 tgcgatcctg gcgctggttt taggtgccgg tctggcggag cgcgtcggct tactgccagc
    11761 gctaatggtt aaaatggcat cgcatgttaa tgtccgctac gccagttata tggtgctgtt
    11821 tattgctttt ttcagccaca tttcttccga tgcggcgtta gtgatcatgc caccgatggg
    11881 tgcgctgatt tttctggcgg tgggcaggca tccagttgca ggtttactgg ctgccattgc
    11941 aggcgtaggt tgcggcttta cggctaattt actgattgtc acaaccgacg tgttgctgtc
    12001 ggggatccgc acggaagcgg cggctgcgtt caatccgcaa atgcacgtca gtgtaattga
    12061 taactggtat tttatggcca gctccgtagt cgtactgacg attgttggcg gcctgataac
    12121 cgacaaaatc atcgagccac ggttaggtca atggcaggga aacagcgatg agaaactgca
    12181 gacattgacc gaaagtcagc gttttggttt acgcatagca ggtgtcgtat cgctactttt
    12241 tattgctgcg atcgcgctga tggtgatccc ggaaaacggg atattgcgcg atccgattaa
    12301 tcacaccgtg atgccatcac cctttattaa aggtatcgtg ccactaatca ttcttttttt
    12361 ctttgtggtt tcgctggctt atggcatcgc tacccgcaca attcgacatc aggcggattt
    12421 accgcattta atgattgaac cgatgaaaga gatggcggga tttatcgtga tggtttttcc
    12481 cctcgcccag tttgtcgcca tgtttaactg gagcaacatg gggaaattca tcgccgtggg
    12541 gctgaccgat atactggaaa gttcagggct tagcggcatc ccggcgtttg tcggtctggc
    12601 gttgctttcc tctttcttat gcatgtttat tgccagtggt tccgcaatct ggtcgattct
    12661 ggcccccatt ttcgtaccaa tgtttatgct acttggcttt cacccggcat ttgcgcaaat
    12721 cctctttcgt attgccgact catccgtatt gcctttagcg ccggtatctc cttttgttcc
    12781 actgtttctt ggattcctgc aacgctacaa accagacgcg aaactgggta cttactattc
    12841 gttagtcttg ccctatccgc ttatcttttt ggtggtatgg ctgctgatgt tgctggcgtg
    12901 gtatcttgtt ggtctgccga taggtccggg gatttaccca cgtttgtctt aagagagaac
    12961 ggatgctgag attacttgaa gaaaaaattg ccacgccact gggtccactg tgggtgattt
    13021 gcgatgagca atttcgcctg cgggcggttg aatgggaaga gtacagcgaa cgcatggtgc
    13081 agctgctgga catccattat cgcaaagaag gctatgagcg catttctgcc accaacccag
    13141 gcggtttaag cgacaagctt agtgattatt ttgccggtaa tcttagcatt attgatacgc
    13201 ttcccactgc cacagggggg acgccatttc agcgcgaagt ctggaaaact ctacgcacta
    13261 tcccctgcgg gcaggtaatg cattacggcc aactggctga acaattgggc cgccctggcg
    13321 cggcgcgtgc cgttggtgcg gcaaacggat cgaatcccat cagcatcgtc gtaccttgcc
    13381 atcgggttat tggccgaaac tgcaccatga ccggatatgc aggcggagtt cagcgaaaag
    13441 agtggttatt gcgccacgaa ggttatcttt tgctgtaaac attaaacaat ttgtgccagc
    13501 ttgttcacac ttttatgtaa agttaccctt aacaacttaa tggttttcaa atagatagac
    13561 atatatttac atctaatatc ggaattctct gctgttaagg tttgcttaga cttacttgct
    13621 ccctaaaaag atgttaaaat tgacaaatat caattacggc ttgagcagac ctatgatccc
    13681 ggaaaagcga attatacggc gcattcagtc tggcggttgt gctatccatt gccaggattg
    13741 cagcatcagc cagctttgca tcccgttcac actcaacgaa catgagcttg atcagcttga
    13801 taatatcatt gagcggaaga agcctattca gaaaggccag acgctgttta aggctggtga
    13861 tgaacttaaa tcgctttatg ccatccgctc cggtacgatt aaaagttata ccatcactga
    13921 gcaaggcgac gagcaaatca ctggtttcca tttagctggc gacctggtgg gatttgacgc
    13981 catcggcagc ggccatcacc cgagcttcgc tcaggcgctg gaaacctcga tggtatgtga
    14041 aatcccgttc gaaacgctgg acgatttgtc cggtaaaatg ccgaatctgc gtcagcagat
    14101 gatgcgtctg atgagcggtg aaatcaaagg cgatcaggac atgatcctgc tgttgtcgaa
    14161 gaaaaatgcc gaggaacgtc tggctgcatt catctacaac ctgtcccgtc gttttgccca
    14221 acgcggcttc tcccctcgtg aattccgcct gacgatgact cgtggcgata tcggtaacta
    14281 tctgggcctg acggtagaaa ccatcagccg tctgctaggt cgcttccaga aaagcggtat
    14341 gctggcagtc aaaggtaaat acatcaccat cgaaaataac gatgcgctgg cccagcttgc
    14401 tggtcatacg cgtaatgttg cctgattttt ccgcataact cactatcctt ctgtcatatc
    14461 attaaatttt tctgatttat tgatctggca gaaggttcat cactgtttca ttcaccagat
    14521 atgggttaat cttttaatta caaactgcgt tgacagttgt tgtaaggaga ccctgtatgg
    14581 ctatgtatca gaacatgctc gttgttatcg atcctaacca ggacgaccaa ccagcattgc
    14641 ggcgagctgt ttatttacat caacggattg gtggcaaaat taaagccttt ttgccgatct
    14701 atgacttctc atacgaaatg accaccctgc tctccccgga cgaacgtacc gctatgcgtc
    14761 agggcgtcat cagccagcgt acagcctgga tccacgagca ggcaaaatat tatctcaatg
    14821 ctggcgttcc cattgaaatt aaagtggtct ggcataaccg tcctttcgaa gccatcattc
    14881 aggaagtgat cagcggcggg cacgatttgg tgctaaaaat ggcgcaccaa catgaccgtc
    14941 tggaagcggt gatttttacg ccaacggact ggcatctgtt acgcaaatgc ccaagcccgg
    15001 tgtggatggt gaaagaccag ccgtggccgg aaggaggtaa ggcgctggtg gcggtgaatc
    15061 tcgccagtga agagccgtat cataatgcgc tcaatgaaaa actggtcaaa gagacgatcg
    15121 aactggcaga acaagtcaac cataccgaag ttcatctggt tggcgcttac ccagtaacgc
    15181 caattaatat cgcgattgaa ctgccggaat ttgagccgag cgtttataac gatgccattc
    15241 gtgggcaaca tttgctggca atgaaagccc tgcggcagaa attcggcatt aatgaaaaca
    15301 tgacgcacgt agaaaaaggt ctgcccgaag aggttattcc tgatttggcg gagcacttac
    15361 aggcggggat tgtggttctg ggcacggtag gacgcaccgg tatttcagca gcattcctcg
    15421 gcaacacggc ggaacaggtg attgatcatc ttcgctgcga tctgttggtc attaaacctg
    15481 accagtatca gacacccgtt gaactggatg acgaagaaga cgattaacga ctcttcaata
    15541 actgacaagg cccgagttat cgggcctatt ttttacttac tgctcttcgc cgccaaggaa
    15601 ataaattccc aacgggattg ccagcaacac agtaaagacc aggctataaa caaaaatcat
    15661 cgatgactga atcacataca ttgatgtcgt ccactcagac agcggaatat tgtactgttc
    15721 tataacgccg ccgatagtcg cgcgagtcac aaccgacgcc gcaataatga caaaccgcca
    15781 acaaaccaaa gagaaatttc ttccctttcg ctgacttcag tttcgccata atcatgatgt
    15841 aatccttgtg cagacaaatt tcttgttacc taaacgtaac aataccatga catttcaccg
    15901 ttgtctgctc tgaaaacctc acctcttagc tggatttaca atcaacgcat tgattagtt
//
LOCUS       DAZPEB010000090        15858 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZPEB010000090 DAZPEB010000000
VERSION     DAZPEB010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15858
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.091"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            155..466
                     /locus_tag="ACHURJ_002413"
                     /pseudo
     CDS             155..466
                     /locus_tag="ACHURJ_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417302.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            463..870
                     /locus_tag="ACHURJ_002414"
     CDS             463..870
                     /locus_tag="ACHURJ_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFR1281323.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAETDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGNIVFSPSGWSDFCPLKERALCQLP"
     gene            855..1178
                     /locus_tag="ACHURJ_002415"
     CDS             855..1178
                     /locus_tag="ACHURJ_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFR1281324.1"
                     /translation="MSASLKNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRLQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            1191..4559
                     /gene="recC"
                     /locus_tag="ACHURJ_002416"
     CDS             1191..4559
                     /gene="recC"
                     /locus_tag="ACHURJ_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFR1281325.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKLGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEHEDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPEWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRLWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGLSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDNI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            4735..7623
                     /gene="ptrA"
                     /locus_tag="ACHURJ_002417"
     CDS             4735..7623
                     /gene="ptrA"
                     /locus_tag="ACHURJ_002417"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFR1281326.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTQARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTTEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            7616..11158
                     /gene="recB"
                     /locus_tag="ACHURJ_002418"
     CDS             7616..11158
                     /gene="recB"
                     /locus_tag="ACHURJ_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFR1281327.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIATVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPKSVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNEKGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            11158..12984
                     /gene="recD"
                     /locus_tag="ACHURJ_002419"
     CDS             11158..12984
                     /gene="recD"
                     /locus_tag="ACHURJ_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFR1281328.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     TLTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(13046..14377)
                     /gene="argA"
                     /locus_tag="ACHURJ_002420"
     CDS             complement(13046..14377)
                     /gene="argA"
                     /locus_tag="ACHURJ_002420"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFR1281329.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            14609..>15858
                     /gene="amiC"
                     /locus_tag="ACHURJ_002421"
     CDS             14609..>15858
                     /gene="amiC"
                     /locus_tag="ACHURJ_002421"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFR1281330.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
BASE COUNT         3738 a         3962 c         4434 g         3724 t
ORIGIN      
        1 ccatttgaag tttaaactca tttgaaaatg atctgcgggg ttctgcagat aatgctttct
       61 gttccataac aggtgtccac tagttgaaaa ggtgggcacc tacgttacca atacaggctt
      121 aatggctaca tacggcggtc agtttacgct tacctgtatc attgtgcagt gggatgcgaa
      181 cagtaacggt atctgggatc gcgaaccggt aaaagagtcc gaccagattg gatttcgtct
      241 gaaggagcat gtgctggaaa cgctacgcgg tgcgacatcc tgtgaaggta agggctggga
      301 taaagtcact aatccggatg ccatcattat cgacactttt caggtcgtac gtcaggatgt
      361 cagcggcttc tcgccggtgt tgacggttaa tatgcgtgct gccagtaagt ctgaaccgca
      421 aaccgtggtg aatgccagct atagcgtgac aggattcaac ctgtgaaccg cgaaaagggt
      481 gtttcgtcac tggctctggt cctgatgctg ctggttttgg gtagtttgct attacaagga
      541 atgagccaac aggatcgcag ttttgcctct cgcgtgagca tggaaagtca gtcattgcgc
      601 cgccaggcca tcgttcagtc ggcgctggcg tggggaaaaa tgcactcctg gcagacgcag
      661 ccagcagttc agtgctcgca gtacgctgaa accgatgccc aggtttgttt gcgtttactg
      721 gcagataatg aagccttatt gattgccggt tatgaaggcg tttcgttgtg gcgaacaggc
      781 gaagtcattg atggaaacat tgttttttcg ccaagcggct ggagcgattt ttgtccgctg
      841 aaagagaggg cgttatgtca gcttccctga agaatcaaca aggctttagc ctgccggagg
      901 taatgttggc gatggtgttg atggtgatga ttgtcactgc gttatcgggt ttccagcgaa
      961 cattaatgaa cagtcttgcc agcagaaacc agtaccaaca gctctggcgg catggctggc
     1021 agcaaacgca actgcgcgcg atttcgccac cagctaactg gcaggtcaac cgattgcaga
     1081 catcgcaggc gggatgtgtc agcatcagcg ttacgctagt ttcacccggg ggcagagaag
     1141 gcgagatgac ccgcctgcat tgcccgaatc gtcagtagtc aggagccgct atgttaaggg
     1201 tctaccattc caatcgtctg gacgtgctgg aagcgttgat ggagtttatt gtcgaacgcg
     1261 aacggctgga cgatcctttc gaaccagaga tgattctggt gcaaagtacc ggtatggcac
     1321 agtggctgca aatgaccctg tcgcaaaagt taggtattgc ggcaaacatt gattttccgc
     1381 tgcctgcgag ctttatctgg gatatgttcg tccgggtgtt accggagatc cccaaagaga
     1441 gcgcctttaa caaacagagc atgagctgga aactgatgac tctgctgccg caactgttgg
     1501 agcacgaaga ctttaccctg ttgcggcatt atctgactga cgatagcgac aagcgaaaac
     1561 tgttccagct ttcctcaaaa gcggcggacc tgtttgacca gtatctggtc tatcgcccgg
     1621 agtggctggc acaatgggaa acaggacatt tggttgaagg gctgggagaa gcacaggcct
     1681 ggcaagcgcc gttgtggaag gcgctggtgg aatataccca tgaactcgga caaccgcgct
     1741 ggcaccgcgc gaatctctat cagcgcttta tcgaaacgct ggagtccgcg acgacctgcc
     1801 cgccggggtt accttcgcgc gtctttatat gcggtatttc cgcgttaccg cctgtttatc
     1861 tccaggcgct ccaggcgctg ggtaaacata ttgaaatcca tctcctgttt accaacccct
     1921 gccgttatta ctggggcgat attaaagatc ctgcttatct ggcgaaactg ctgactcgtc
     1981 agcgtcgaca cagttttgaa gatcgcgaat tgccgctatt tcgcgatagc gaaaatgccg
     2041 gacagctctt taacagcgat ggtgaacagg atgtcggcaa cccgctgctg gcctcatggg
     2101 ggaagcttgg gcgcgactac atttatctcc tttctgacct ggagagcagc caggagctgg
     2161 acgcctttgt cgatgtcacg ccagataacc tgctgcataa cattcagtct gacattctgg
     2221 aactggaaaa ccgtgccgtt gctggtgtga acatcgaaga gttttcccgt agcgataaca
     2281 aacgcccgct tgatccactg gatagcagta tcaccttcca cgtttgccat agcccgcagc
     2341 gtgaagttga agttttgcac gatcgcctgc tggcgatgct ggaagaagac ccgacactta
     2401 ctccgcgcga catcatcgtg atggtggctg atatcgacag ctacagtccg tttattcagg
     2461 ctgtgtttgg tagcgcacct gcggatcgtt acctgcctta cgccatttcc gaccgtcggg
     2521 cgcggcagtc gcatccggta ctggaagcgt ttatcagcct gttatcgttg ccagacagcc
     2581 gctttgtgtc ggaagacgtg ctggcattac tggatgtgcc ggtgctggcg gcgcggtttg
     2641 acatcaccga agaagggctg cgttatttac gcctgtgggt caacgaatcc ggcattcgtt
     2701 gggggataga tgacgacaac gttcgcgagc tggaactccc cgccaccgga caacacacct
     2761 ggcgatttgg cctgacgcgt atgttgctgg gctacgcgat ggagagcgcg cagggcgagt
     2821 ggcaatcggt tctaccttat gatgagtcga gcggcttaat tgcagaactg gtggggcatc
     2881 tggcttcact gctaatgcag ctaaacatct ggcgtcgcgg gctggcacag gagcgtccgc
     2941 tagaagagtg gttgccggtt tgtcgcgata tgctcaacgc cttcttcctg ccggatgcgg
     3001 aaaccgaagc ggcgatgacg ctgatcgaac aacaatggca ggcgattatc gccgaaggtt
     3061 taggtgcgca gtatggcgac gcggtgccgc tgtcactatt gcgtgatgaa ctggcacagc
     3121 gtctggatca agaacgtatc agccagcgtt ttctcgccgg accggttaac atttgtactc
     3181 tgatgccaat gcgttcaatt ccgttcaaag tggtttgcct gctgggaatg aacgacggcg
     3241 tttatccacg tcagcttgcg ccattgggct ttgacctgat gagccagaaa ccgaagcgtg
     3301 gcgaccgtag ccgtcgcgat gacgaccgct acctgttcct ggaagcgtta atttccgcgc
     3361 agcaaaaact ctatatcagt tatatcgggc tttccattca ggataacagt gaacgtttcc
     3421 cgtcggtact ggtgcaggaa ctgatcgact acatcgggca aagtcattat ctaccgggcg
     3481 atgaagcgct caactgtgat gaaagcgagg caagggtaaa agcgcatctt acctgcctcc
     3541 atacccggat gccgtttgat ccacaaaact accagccagg cgaacgacaa agctatgctc
     3601 gtgaatggct acctgcggcc agccaggctg gtaaagcaca ttctgaattt gttcagccgc
     3661 tgccgtttac cttaccggaa accgtgccgc tggaaacgct acaacgattc tgggcacatc
     3721 cggtgcgggc atttttccag atgcgtttgc aggtgaactt ccgtaccgaa gacagcgaaa
     3781 tccccgacac cgagccattt attctggaag ggcttagccg ttatcaaatc aatcagcagt
     3841 tattgaatgc actggttgag caggatgatg ccgaacgctt gttccgtcgc ttccgggcgg
     3901 caggggattt accgtatggc gcttttggtg aaattttctg ggaaactcag tgccaggaga
     3961 tgcagcagct tgccgacaga gtcattgcct gtcgccagcc ggggcagagt atggaaattg
     4021 atctcgcctg caacggtgtg cagataactg gctggttgcc gcaggtgcag ccggatggcc
     4081 tgttgcgctg gcgtccctct ttattaagtg tggcgcaggg aatgcaactt tggctggaac
     4141 accttgtcta ctgtgccagc ggtggtaatg gtgaaagtcg cctttttcta cgcaaagacg
     4201 gcgagtggcg ttttccgccg cttgcagccg aacaggcttt gcattacctc tcacaactga
     4261 ttgaggggta tcgtgaaggg atgtccgcgc cattgctggt gttacctgaa agtggcggcg
     4321 cgtggctaaa aacctgttat gacgcgcaaa acgatgccat gctggatgac gattccacgt
     4381 tgcaaaaagc ccgtacgaaa ttccttcagg cttacgaagg caacatgatg gtgcgtggcg
     4441 aaggtgataa tatctggtat caaaggctct ggcggcaatt aacaccagag acaatggagg
     4501 ccatcgttga acagtcgcaa cgtttcctgt taccgctgtt tcgctttaat cagtcatgag
     4561 tgctgtataa aaattgcgca atctatccgc ttactttatg atgcgcacca gtcacggact
     4621 gatggttata taaacatagg ctgactcgtg cagcacaaga ttaaattctg gcagatgatt
     4681 tgcgttaacg tgttgaatct ggacagaaaa ttaagttgat tatgaggtcc gtgaatgccc
     4741 cgcagcactt ggttcaaagc attattgttg ttagttgccc tttgggcacc cttaagtcag
     4801 gcagaaacgg gatggcagcc gattcaggaa accatccgta aaagtgataa agataaccgc
     4861 cagtatcagg ctatacgtct ggataacggt atggtggtct tgctggtttc tgatccgcag
     4921 gcagttaaat cgctctcggc gctggtggtg cccgttgggt cgctggaaga tcccgaggcg
     4981 taccaggggc tggcacatta ccttgaacat atgagtctga tggggtcgaa aaagtacccg
     5041 caggctgaca gtctggccga atatctcaaa atgcacggcg gtagtcacaa tgccagcact
     5101 gcgccgtatc gcacggcttt ctatctggaa gttgagaacg acgccttgcc tggtgcggta
     5161 gaccgcctgg ccgatgctat tgctgaacct ttgctcgaca agaaatatgc cgaacgtgag
     5221 cgtaatgcgg tgaacgctga attaaccatg gcgcgtacgc gtgacgggat gcgcatggca
     5281 caggtcagcg cagaaaccat taacccggca caccccggtt caaagttttc tggtggtaac
     5341 ctcgaaactt taagcgataa acctggtaat ccggtgcagc aggcgctgaa agatttccac
     5401 gagaagtact attccgccaa tctgatgaag gcggttattt acagcaataa accgctgccg
     5461 gagttggcaa aaatggcggc ggacaccttt ggtcgcgtgc cgaacaaaga gagcaaaaaa
     5521 ccggaaatca ccgtgccggt agtcaccgac gcgcaaaagg gcattatcat tcattacgtc
     5581 ccggcactgc cgcgtaaagt tctgcgcgtt gagtttcgca tcgataacaa ctcagcgaag
     5641 ttccgtagta aaacggatga attgattacc tatctgattg gtaatcgcag cccaggtaca
     5701 ctttccgact ggctgcaaaa gcagggatta gttgagggca ttagcgccaa ctccgatcct
     5761 atcgtcaacg gcaacagcgg cgtattagcg atctctgcgt ctttaaccga taaaggcctg
     5821 gctaatcgcg atcaggttgt ggcggcaatt tttagctatc tcaatctgtt acgtgaaaaa
     5881 ggcattgata aacaatactt cgatgaactg gcgaatgtgc tggatatcga cttccgttat
     5941 ccgtcgatca cccgtgatat ggattacgtc gaatggctgg cagataccat gattcgtgtt
     6001 ccggttgagc atacgctgga tgcagtcaat attgccgatc ggtacgatgc taaagcagta
     6061 aaagaacgtc tggcgatgat gacgccgcag aatgcgcgta tctggtatat cagcccgaaa
     6121 gagccgcaca ataaaacggc ttattttgtc gatgcgccgt atcaggtcga taaaatcagc
     6181 gaacaaactt tcgctgactg gcagcaaaaa gctgccaata ttgcgctctc cttaccggag
     6241 cttaacccct atattcctga cgatttctcg ctgattaagt cagagaagaa atatgaccat
     6301 ccagagctga ttgttgatga gtcgaatctg cgcgtggtgt atgcgccaag ccgttatttt
     6361 tccagcgaac ccaaagctga tgtcagcctg attttgcgta atccgaaagc catggacagc
     6421 gcccgcaatc aggtgatgtt tgcgctcaat gattatctcg cagggctggc gcttgatcag
     6481 ttaagcaacc aggcgtcggt tggtggcata agtttttcca ccaacgctaa caacggcctt
     6541 atggttaatg ctaatggtta cacccagcgt ctgccgcagc tgttccaggc attgctcgag
     6601 gggtacttta gctataccgc tacggaagat cagcttgagc aggcgaagtc ttggtataac
     6661 cagatgatgg attccgcaga aaagggtaaa gcgtttgagc aggcgattat gcccgcgcag
     6721 atgctctcgc aagtgccgta cttctcgcga gatgaacggc gtaaaatttt gccctccatt
     6781 acgttgaaag aggtgctggc ctatcgcgac gccttaaaat caggggctcg accagagttt
     6841 atggttatcg gcaacatgac cgaggcccag gcaacaacgc aggcacgcga tgtgcaaaaa
     6901 cagttgggcg ctgatggttc agagtggtgt cgaaacaaag atgtagtggt cgataaaaaa
     6961 caatccgtca tctttgaaaa agccggtaac agcaccgact ccgcactggc agcggtattt
     7021 gtaccgactg gctacgatga atacaccagc tcagcctata gctctctgtt ggggcagatc
     7081 gtacagccgt ggttctacaa tcagttgcgt accgaagaac aattgggcta tgccgtgttt
     7141 gcgtttccaa tgagcgtggg gcgtcagtgg ggcatgggct tccttttgca aagcaatgat
     7201 aaacagcctt cattcttgtg ggagcgttac aaggcgtttt tcccaaccac agaggcaaaa
     7261 ttgcgagcga tgaagccaga tgagtttgcg caaatccagc aggcggtaat tacccagatg
     7321 ctgcaggcac cgcaaacgct cggcgaagaa gcatcgaagt taagtaaaga tttcgatcgc
     7381 ggcaatatgc gcttcgattc gcgtgataaa atcgtggccc agataaaact gctgacgccg
     7441 caaaaacttg ctgatttctt ccatcaggcg gtggtcgagc cgcaaggcat ggctattctg
     7501 tcgcagattt ccggcagcca gaacgggaaa gccgaatatg tacaccctga aggctggaaa
     7561 gtgtgggaga acgtcagcgc gttgcagcaa acaatgcccc tgatgagtga aaagaatgag
     7621 tgatgtcgcc gagacactag atcctttgcg cttgccctta cagggtgagc gcctgattga
     7681 agcctctgcc ggcacaggca aaacctttac gattgcggcg ctctatttgc gcctgttact
     7741 tggactaggc ggttccgccg cctttccccg cccgctgacc gttgaagaac tgctggtggt
     7801 cacctttacc gaggctgcca cggcagaatt gcgtggtcgt atccgtagca atatccatga
     7861 gttgcgcatc gcctgtctgc gtgaaactac cgacaatcca ctgtacgaac gtctgctgga
     7921 agagatcgac gataaagcgc aagccgcgca gtggttgttg ttagccgaac ggcagatgga
     7981 tgaagcggca gtctttacta ttcacggttt ttgccagcgc atgctcaacc tgaatgcctt
     8041 tgaatccggc atgctgtttg agcagcagct gattgaagat gagtctctgc tacgttacca
     8101 ggcctgcgcc gatttctggc gtcgccactg ctacccgctg ccgcgtgaaa tagcccaggt
     8161 cgtctttgaa acctggaaag ggccgcaggc gttgctgcgc gatattaatc gttatctgca
     8221 aggcgaagcg ccggttatca aagcaccgcc gcccgatgat gaaacgctgg cttcccgcca
     8281 cgcgcaaatt gtggcgcgta ttgctacggt aaaacagcag tggcgcgacg cagtgggtga
     8341 actggatgcg ctgatcgaat cttctggtat tgatcgacgc aagtttaacc gtagcaatca
     8401 ggctaaatgg atcgacaaga tcagcgcctg ggcagaagaa gagaccaaca gttatcagtt
     8461 gccggagtcg ctggaaaaat tctcccagcg tttcttagaa gatcgcacga aagctggggg
     8521 ggaaaccccg cgacatccac tgtttgaggc gatcgatcaa ctgcttgcag aaccattgtc
     8581 gatccgcgat ctggtgatca cccgcgcatt ggctgagatc cgcgaaacag tagcgcgtga
     8641 aaaacgccgc cgtggcgaat tgggttttga tgacatgtta agtcggctcg attccgcgct
     8701 gcgtagcgaa agcggtgagg tgttggcagc ggcgatccgt acgcgattcc cggtggcaat
     8761 gatcgatgaa tttcaggata ccgaccccca gcagtaccga atttttcgcc gtatctggca
     8821 ccatcagccg gaaaccgcat tgttgctgat tggcgacccg aagcaggcca tatatgcatt
     8881 ccggggtgcg gatatcttca cttatatgaa ggcgcgtagt gaagttcacg cccactacac
     8941 tttagacacc aactggcgct ccgcacctgg aatggtgaac agtgtgaaca agcttttcag
     9001 ccagactgat gacgcgttca tgtttcgcga aataccgttt attccagtga aatcagccgg
     9061 gaaaaatcag gcgttacgtt ttgtatttaa aggtgaaacg cagcctgcga tgaaaatgtg
     9121 gctgatggaa ggcgaaagct gcggcgttgg cgactatcaa agtaccatgg cgcaggtatg
     9181 tgctgcgcaa atccgcgact ggctacaagc cggacagcgg ggcgaagcgt tgctgatgaa
     9241 cggcgacgac gcgcgtccgg tgcgtgcttc ggacatcagt gtgctggtgc gcagccgcca
     9301 ggaggccgcc caggtgcgcg atgccttaac gttgctggaa atcccttccg tttacctttc
     9361 gaaccgcgac agtgtttttg aaactctgga agcgcaggaa atgctttggt tattgcaggc
     9421 ggtgatgacg cccgaacgtg agaacaccct gcgtagtgcg ctggcaacgt caatgatggg
     9481 gctgaacgcg ctggatatcg aaacgctgaa taatgacgaa catgcgtggg atgcggtagt
     9541 tgaagagttc gatggttatc ggcaaatctg gcgcaaacgt ggcgttatgc cgatgctgcg
     9601 ggcgctgatg tcggcgcgta acattgcaga aaacttgctg gcaacggcag gcggtgagcg
     9661 gcgtcttacc gatatcttgc atatcagcga actgctacaa gaagccggaa cgcagctgga
     9721 aagtgaacat gcgctggtac gctggttatc gcaacatatc ctcgagccag acagtaatgc
     9781 ctccagccaa caaatgcgcc tcgaaagtga taaacatctg gtgcagattg tcacgatcca
     9841 taaatcgaaa gggctggaat atccgctggt ctggttgcca tttattacca atttccgcgt
     9901 ccaggatcag gcgttttatc acgatcgcca ctcgtttgag gcagttttgg atcttaatgc
     9961 tgcgccaaaa agcgtcgacc tcgcggaggt cgaacgtctg gcggaagatc tgcgtttgct
    10021 ttacgtggcg ctgacgcgtt cggtttggca ttgcagtctc ggcgttgcac cgctggtgcg
    10081 ccgtcgtggc gataaaaaag gtgacaccga cgtccaccaa agtgcgctcg ggcgtttgct
    10141 gcaaaaaggg gaaccgcaag atgcggcagg gcttcgcacc tgtattgaag cgttatgcga
    10201 tgatgatatt gcctggcaaa cggcacaaac tggtgataac cagccctggc aggttaatga
    10261 tgctttaact gcagaactga atgcgaggac actacaacga ttgcccggcg ataactggcg
    10321 tgtcaccagc tactccggct tgcagcagcg tggtcacggt atcgctcagg atctgatgcc
    10381 acggctggat gttgatgccg caggcgtggt cagcgtcgtt gaagaaccga cgttaacacc
    10441 gcatcagttc ccgcgcggtg cgtcaccggg gacattcttg cacagtttgt ttgaagacct
    10501 cgattttacc cagccggttg acccgaactg ggtacaggaa aaactggaac tcggcggctt
    10561 tgaatcgcag tgggaaccgg tgttgaccga gtggatcacg gctgtcctcc aggcacctct
    10621 caatgaaaag ggggttagcc tgagtcagct ttccgaccgc gataaacagg tggagatgga
    10681 gttttacctg ccgattagcg aaccgctcat cgccagccag cttgatgcac taattcgcca
    10741 gtttgatccg ctatccgctg gctgcccgcc gctggagttc atgcaggtac gcggcatgtt
    10801 aaaaggcttt atcgacctag tattccgcca cgaagggcgt tattacctgc tcgactataa
    10861 atccaactgg ctgggtgaag acagttcggc ttacacccaa caggctatgg cagcagcgat
    10921 gcaggcacac cgctatgatc tgcaatatca gctttatacc ctggcgctgc atcgttatct
    10981 gcgccatcgc attgctgatt acgactatga tcgtcacttt ggcggcgtta tctatctgtt
    11041 cctgcgtggc gttgataaag aacatccgca acaagggatt tacacaaccc gacccaacgc
    11101 cgggttgatt gccctgatgg atgagatgtt tgccggtatg accctggagg aggcgtaatg
    11161 aaattgcaaa agcaattact ggaagctgtg gagcacaaac agctacgccc gctggatgtg
    11221 caatttgccc tgaccgtggc gggagatgaa catcctgccg tcaccctcgc ggcggcactg
    11281 ttaagtcatg atgccggaga gggacacgtc tgtttgccgc tttcacgact ggaaaataac
    11341 gaggcgtcgc atccgctgtt ggcgacctgt gtcagtgaaa tcggtgagct acaaaattgg
    11401 gaagaatgct tgctggcttc tcaagcggtc agcaggggag atgaacccac gccgatgatc
    11461 ctctgtggcg atcgtcttta tttgaatcgc atgtggtgta acgagcggac ggtagcgcgc
    11521 ttcttcaatg aggtgaatca tgccattgag gttgatgaag ctctactggc gcaaaccctg
    11581 gacaaacttt ttccagtaag cgatgaaatt aactggcaaa aagttgcagc ggcagtgacg
    11641 ctgacgcggc ggatctcggt gatttccggc ggcccaggca ccggtaaaac gaccaccgtg
    11701 gcgaagttgc tggcagcgtt aattcaaatg gccgacggcg aacgctgccg tatccgtctg
    11761 gctgcaccaa cgggtaaagc tgccgcgcgc ttaaccgaat ctctcggcaa ggctttgcga
    11821 cagttaccgc tgaccgatga acaaaagaaa cgcattccgg aagatgccag cactttgcac
    11881 cgattgctgg gcgcgcagcc gggtagccag cgtttacgtc atcatgccgg taacccgctg
    11941 catcttgatg tgctggtggt agatgaagcg tcaatgatcg atctgcctat gatgtcgaga
    12001 ctgatcgacg ccttgcccga tcatgcgcga gtgatctttc tcggcgatcg tgatcaactg
    12061 gcctcggttg aggctggggc tgtgctgggc gatatctgcg cttatgccaa cgcgggcttt
    12121 accgccgagc gtgccaggca gctaagccgc ctgacgggga ctcacgttcc ggcaggaacc
    12181 ggcacagaag cggcatcttt gcgcgacagt ctctgcctgc tgcaaaaaag ctatcgtttc
    12241 ggcagcgatt ctggcattgg tcagttagct gcggcgatca accgtggtga taaaacggca
    12301 gtgaaaaccg tttttcagca ggattttact gatatcgaaa aacgactttt acagagcggc
    12361 gaagattata ttgcgatgct tgaggaagct cttgcgggtt atggacgtta tctggatctg
    12421 ctgcaagcgc gtgccgagcc ggatttaatc attcaggcgt tcaatgagta ccaacttttg
    12481 tgcgccctgc gggaagggcc gtttggcgtg gctggactga atgagcgaat tgagcagttt
    12541 atgcaacaga agcgcaaaat tcatcgtcat ccgcactctc gttggtacga aggtcgaccg
    12601 gtgatgattg cccgtaatga cagcgcgctt gggttgttta atggcgatat cggtattgcg
    12661 ctggatcgcg ggcaggggac gcgcgtctgg tttgcgatgc cggacggcaa tattaagtct
    12721 gtgcaaccga gtcgcttgcc agagcacgaa actacgtggg cgatgacggt acataaatcg
    12781 cagggatcgg agttcgacca tgcggcgttg attttaccga gccaacgcac gccggtagta
    12841 acgcgagagc tggtatacac cgcggtgacc cgcgcgcgtc gccgtctgtc gctgtatgcc
    12901 gatgagcgta tattaagtgc ggcaattgcc actcgtactg agcggcgcag tggtctggcg
    12961 gcgttgttta gttcgcggga ataaacgtaa ttgccggatg cgatgcacga gtgttacgca
    13021 tgtcgcatcc gacaattttc atcgcttacc ctaaatccgc catcaacact ttggatttac
    13081 gctggtagtt gtacaactgc tttttgctct cgggcagtaa atcaatatcc accggggtaa
    13141 atccacgttc ctggaaccag tgaatactgc gcgtggtcag cacaaacaat ttgcttaagc
    13201 cgctctgctt cgcctgagcg gcaatgcgtt ccagcagaac ctcgccccgt gatgaactgc
    13261 ggtaatccgg gtgaactgcc acacaggcca tttccccaat cttctcttcc gggaacggat
    13321 agagcgcggc gcaggcaata gtcgtgttat cgcgctgaat aatggtgaat ttgtcgattt
    13381 ccatctccag ctgctcgcga gaacggcgca ccagaatacc ttgctgctcc agcgggcgaa
    13441 tcaactccag aataccgcca atatcgttga ttgttgcgcg acgaatctgc tcggcgcttt
    13501 ccatcacaat ctgcgtaccg ataccgtcgc gtgagaacaa ctcttgcaac agcgcgccat
    13561 cttcctgata actgattaaa tgacagcgac gcacgccgct gcggcaggct ttcactgcgc
    13621 cacgcaaaaa gcgcaccgta ccggagttgt aatcgccttt ctcttcctgg gcttctaccc
    13681 gcgcttgcgc ttcgttaggg aaaagttctg agacaatatc accgtcgtca ttagtgacgc
    13741 cctgtgaaga gcaaaaacca atcatcttct cagctttcag tttgatggcc agttgagtgg
    13801 caatctcttc cgaggtcaga ttaaagctct cgccagtgac tgaaacagcg accggcccca
    13861 ttagcactat tgcaccgctg tccagttgac gatggatcgc gtcttcatca atccgccgga
    13921 tacgcccgct atggcagtaa tccacgccgt catcgacgcc cagcggctgg gcaataataa
    13981 aattgccact gacgacgttg atatgcgcgc cctgtagcgg cgtgttattg agactcatcg
    14041 acaggcgagc agtgatgtcc agttgcaatg ttcccgcagc ctgcttcacc agttccagtg
    14101 ttttggcgtc ggtcacacgt atattcttgt gatacagcgg ttcgtggtga tgcgcggcca
    14161 gatttgcgtc gatctgcgga cgtgcgccat agaccaccac cagacggatg ccgaggctgt
    14221 gcaacaaccc gatatcatta acgatactgg agaaattctc atgctcaatg gcttcaccgc
    14281 cgagcatgat gacaaacgtt tttccccggt gggtattgat atagggaacc gaatggcgga
    14341 atccctcgac cagctcggtt ttacgttcct ttaccacggc acaccccttt gcatgattat
    14401 tctaaattag tgtattttta ttctgttttt tcgcagggtg caagtgtaaa ttttatgcga
    14461 gagcgacttt tttatcagta atgccgtgaa tataaaaaga atgtttaccg tttattagat
    14521 gacagattat gcgtctttcg ctaaagtttc cggtcaaatt agtcgtttac ttgttacaca
    14581 gcttagattt cttttgctcg ggagaggcat gtcaggatcc aacactgcaa tcagccgtcg
    14641 tcgtttactg caaggcgcgg gtgccatgtg gctattgagc gtaagtcagg tcagtctggc
    14701 tgcggtcagc caggtcgtgg cggtgcgcgt ctggcctgcg tccagctaca cccgtgtgac
    14761 ggtagaatca aatcgtcagc tgaaatataa gcagttcgcg ttgagtaacc ctgaacgcgt
    14821 ggtggtggat atcgaagatg taaacctgaa ctcggtactc aaggggatgg cggcacaaat
    14881 tcgtgcagac gacccgttca tcaagtcggc gcgcgtcggg caatttgacc cgcagaccgt
    14941 acggatggtt tttgaattaa agcaaaacgt aaaaccgcag ctgtttgccc ttgcgccggt
    15001 cgccgggttt aaagagcgtc tggtgatgga cctctatccg gccaatgcac aggatatgca
    15061 ggacccgctg ctggcgctgc tggaggatta caacaaaggc gacctcgaaa agcaggtgcc
    15121 gccagcacaa agtggtccac aaccgggtaa agcagggcgc gatcgtccta ttgttattat
    15181 gcttgaccct ggccacggcg gcgaagactc cggtgcggtg gggaaataca aaacgcgcga
    15241 aaaagacgtg gtattgcaaa tagctcgccg tctgcgctct ctgatcgaga aagagggcaa
    15301 tatgaaggtg tacatgacgc gcaatgaaga catcttcatt ccgttgcaag tgcgcgtagc
    15361 aaaagcccag aaacagcgtg ctgacctgtt tgtctctatc catgccgacg cctttaccag
    15421 tcgtcagccg agcggttcct ctgtgtttgc gctctcaacc aaaggtgcaa ccagtactgc
    15481 ggcaaaatat ctggcacaaa cccagaacgc ctcggacttg attggtggcg tgagcaaaag
    15541 cggtgaccgc tatgtcgacc acaccatgtt cgatatggtg cagtcgctga ccattgccga
    15601 cagcctgaag tttggtaaag cggtactgaa taagctcggt aaaatcaaca agctgcataa
    15661 aaatcaagtt gaacaggccg ggtttgccgt actaaaggca ccagatattc cctccattct
    15721 ggtcgaaacg gcgtttatta gtaacgttga ggaagagcgt aaactgaaaa cggcgacttt
    15781 ccagcaggaa gttgcggagt ctattcttgc ggggattaaa gcgtattttg ccgatggggc
    15841 gacgctggcg agaagggg
//
LOCUS       DAZPEB010000091        15613 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZPEB010000091 DAZPEB010000000
VERSION     DAZPEB010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15613
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.092"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(131..883)
                     /gene="tcyN"
                     /locus_tag="ACHURJ_002422"
     CDS             complement(131..883)
                     /gene="tcyN"
                     /locus_tag="ACHURJ_002422"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFR1281331.1"
                     /translation="MSAIEVKNLVKKFQGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     LGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(880..1548)
                     /gene="tcyL"
                     /locus_tag="ACHURJ_002423"
     CDS             complement(880..1548)
                     /gene="tcyL"
                     /locus_tag="ACHURJ_002423"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFR1281332.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPILSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(1563..2549)
                     /gene="dcyD"
                     /locus_tag="ACHURJ_002424"
     CDS             complement(1563..2549)
                     /gene="dcyD"
                     /locus_tag="ACHURJ_002424"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFR1281333.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLNLFNTQIEMCDTLTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(2654..3454)
                     /gene="tcyJ"
                     /locus_tag="ACHURJ_002425"
     CDS             complement(2654..3454)
                     /gene="tcyJ"
                     /locus_tag="ACHURJ_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR1281334.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(3542..4093)
                     /gene="fliZ"
                     /locus_tag="ACHURJ_002426"
     CDS             complement(3542..4093)
                     /gene="fliZ"
                     /locus_tag="ACHURJ_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFR1281335.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQAHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDENGWQVEQQSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(4139..4858)
                     /gene="fliA"
                     /locus_tag="ACHURJ_002427"
     CDS             complement(4139..4858)
                     /gene="fliA"
                     /locus_tag="ACHURJ_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HFR1281336.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLSTKLGKL"
     gene            complement(5178..6884)
                     /locus_tag="ACHURJ_002428"
     CDS             complement(5178..6884)
                     /locus_tag="ACHURJ_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliC/FljB family flagellin"
                     /protein_id="HFR1281337.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRILELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGKGTIANKAATVSDLTAAGATGTGPYAVTTNNTALSASDALS
                     RLKTGDTVTTTGSSAAIYTYDAAKGNFTTQATVADGDVVNFANTLKPAAGTTASGVYT
                     RSTGDVKFDVDANGDVTIGGKAAYLDATGNLSTNNAGIASSAKLSDLFASGSTLATTG
                     SIQLSGTTYNFGAAATSGVTYTKTVSADTVLSTVQSAATANTAVTGATIKYNTGIQSA
                     TASFGGANTNGAGNSNDTYTDADKELTTTASYTINYNVDKDTGTVTVASNGAGATGKF
                     AATVGAQAYVNSTGKLTTETTSAGTATKDPLAALDEAISSIDKFRSSLGAIQNRLDSA
                     VTNLNNTTTNLSEAQSRIQDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLL
                     QG"
     gene            7150..8556
                     /gene="fliD"
                     /locus_tag="ACHURJ_002429"
     CDS             7150..8556
                     /gene="fliD"
                     /locus_tag="ACHURJ_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFR1281338.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAKNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKKQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            8581..8991
                     /gene="fliS"
                     /locus_tag="ACHURJ_002430"
     CDS             8581..8991
                     /gene="fliS"
                     /locus_tag="ACHURJ_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFR1281339.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            8991..9357
                     /gene="fliT"
                     /locus_tag="ACHURJ_002431"
                     /pseudo
     CDS             8991..9357
                     /gene="fliT"
                     /locus_tag="ACHURJ_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015018.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
     gene            9435..10922
                     /gene="amyA"
                     /locus_tag="ACHURJ_002432"
     CDS             9435..10922
                     /gene="amyA"
                     /locus_tag="ACHURJ_002432"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFR1281340.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYNLFDLGEFDQKGSIPTKYGDKVQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVITDEHGEATFFCNGGSVSVWVIEEVI"
     gene            complement(10956..11369)
                     /gene="yedD"
                     /locus_tag="ACHURJ_002433"
     CDS             complement(10956..11369)
                     /gene="yedD"
                     /locus_tag="ACHURJ_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFR1281341.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLALMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            11556..12761
                     /gene="yedE"
                     /locus_tag="ACHURJ_002434"
     CDS             11556..12761
                     /gene="yedE"
                     /locus_tag="ACHURJ_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFR1281342.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            12758..12991
                     /gene="yedF"
                     /locus_tag="ACHURJ_002435"
     CDS             12758..12991
                     /gene="yedF"
                     /locus_tag="ACHURJ_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFR1281343.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVRDIQQDGPTIRYLIQK"
     gene            13098..13769
                     /locus_tag="ACHURJ_002436"
     CDS             13098..13769
                     /locus_tag="ACHURJ_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFR1281344.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPFFIYRADGQPIFMAAIGSTPFEHGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPVC"
     gene            complement(13809..15017)
                     /locus_tag="ACHURJ_002437"
     CDS             complement(13809..15017)
                     /locus_tag="ACHURJ_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR1281345.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALARQNENHE
                     VGNKYIPYGKMASWLVEWKNATETQWLKDAPSQPLQQSLKDLERAYKNFFQNRAAFPR
                     FKKRGQNDVFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTAFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVFAVPPACTSQRC
                     ACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQ
                     EPTEMIQATA"
     gene            15025..15393
                     /gene="tnpA"
                     /locus_tag="ACHURJ_002438"
     CDS             15025..15393
                     /gene="tnpA"
                     /locus_tag="ACHURJ_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFR1281346.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTKYRRQIFDHDATEK
                     LRTYFSNVCADFEAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGVLWSPGYFASS
                     CGGAPISVIRQYIEQQQTPG"
BASE COUNT         3765 a         4053 c         3884 g         3911 t
ORIGIN      
        1 gaattacttt tgcaagccat cgcattctct tatgttatta atgagttatg ctgatttgtt
       61 aagcagtttt atcacgcttg aaatggcgtc ccgccccgac aggtgaatcg tcggggctga
      121 ttttttctta ttattgcagc agaaacttct cgaggaactg tcgggtgcga ggctgctggg
      181 gatcggcaaa taacgctttt gctgccccct gctcgactat ccgccccagg tccataaaaa
      241 tcgcccggtc cgcaacatcc cgggcaaagc tcatttcgtg cgtcacaatc accatcgtgc
      301 gcttttcctg cgccagctgg cggattgtgt tcaggacttc gcccaccagc tctggatcca
      361 gcgctgacgt tggctcgtca aacaaaatca cctcaggacg cattgccagc gcacgcgcaa
      421 tcgctacacg ctgctgttga ccgccagaca aacgacgtgg atagctggtt tctttaccgg
      481 ccagcccaac tttcgccagc agctcgcgag cgcgcgccgt ggcctcttct ttcggttcgc
      541 ctttgacgat caccggccct tcaataatgt tttccagcac cgtacgatgc ggaaacaaat
      601 taaagttctg gaagacaaac ccgacgtgct gacgcaactg gcgaatcagc gatttttgct
      661 gacttaatga acgtgcagta tcaatagtga tatcgccaac ggtgatcgtt cccgcttccg
      721 gttgttccag cagatttatg ctgcgtagca acgtggtttt gccggaacca ctcggaccga
      781 taattgccac cacttcgcca ggctttacct cgaggtcgat accgtgcagc accgtctgac
      841 cttggaattt tttcaccagg ttcttaactt caatggcact catttgggtt ctctctcctg
      901 gcgattaagt tgattctcaa aatggttctg caacgtcgat aacacagtcg ccatgatcca
      961 gtagatgagc gaagccgcca ggtacatggt gaacacctcc agcgtgcgcg aggtaatcaa
     1021 ctgcgcctga cggaacagct ccggcacctg gattgtcgcg gccagcgacg tatcttttac
     1081 cagactgata aagctgttcg acagcggcgg caacgccacc cgcgccgcct gtggcaaaat
     1141 cgcgcgacgc atggtctgcc acggcgtcat accaatactg gcagcggctt cccactgacc
     1201 tttatcaata gaagaaatgg cggcgcgcag cgtttcggcg gcataggcgg cagtgttcag
     1261 cgacagacca atcattgctg acagaatggg atctaattcg ataccaaact gcggcaggcc
     1321 gtaatagatc ataaacagtt gtgcgatgag cggcgtacca cgaaaaatag agatataaaa
     1381 acgcgccagc cagcgcacgg gccagattgg cgacaggcgc atcagcgcga gaataaaccc
     1441 gagtagtaag ccaaaaaaca tgccgccgat actgagttgc agggtatacc cagccccttt
     1501 caacaggaac ggcaaggaat caataaccag ttgtatactt tcttgcatga gcgttcccgg
     1561 agctaaacgt ggggatgata ggcgaacagc gcaggcgcgc cgccggtatg aataaacaga
     1621 atcggccctt catctttgaa gcgtttctga ctgataccgt caatcagccc cgccatcgct
     1681 tttccggtat acacaggatc aagcagaatg ccttccagac gcgccagcaa tttcactgct
     1741 tccatgcctt cgtcgttcgg cacgccgtag ccaggtgcaa aatagtcatc ccagagtaaa
     1801 atttccgctg atgcggtcag ctccagttct ttcgcaatcg cctgttgtag gttaaccact
     1861 ttcggcaatt gatcggcaac ggaacgcgac acggtcacgc caatcagttc gctttcaggc
     1921 atcaggtgtt ccagcccaac agccaatccg gcgtgagttc cggcactgcc cgatgcgacc
     1981 accaccgacg aaatattaac cgccccttca cactgttgcg cgatttccag cgcactctcc
     2041 acataaccca gcgcgcccag agcattagaa ccgccaaccg gaatgacata cgggcgaaag
     2101 ccttgtgctt cgactcgcgt cgccagctct tccagttggg cattgggatc ggtcagtgtg
     2161 tcgcacattt caatctgggt attgaacaga ttcagcaaca aacgattgcc gttggttaaa
     2221 tagttttctg cggttgtgcc aataggattt tccagcagcg ccacgcagtg caggccgagt
     2281 ttcgccgcaa ctgcggcagt ctgccgcaca tggttagact ggatcgcgcc ggcagtaatc
     2341 agcgtatcgg caccttcgcg cagggcatct gccgcgagaa attccagctt acgtaattta
     2401 ttgccgccca ttgccatggg tgtgacgtca tcccgtttga tgaaaatttc ccgtccaaga
     2461 taatcagaaa agcgcggcag atattcgagc ggcgttggcg cgccgataaa ctccagccgt
     2521 ggaaaacggg ttaaattatg cagtggcata atagcctccg atgtgtgttg ttgtgatttt
     2581 cttattatgc acgctgaaaa cgcgtaaata aaaaaggcgc tagtgaaagc gccctttttt
     2641 gtcattatgc tgattatttg gtcacatcag caccaaacca tttttcggaa agggcttgca
     2701 gagtgccatc tttttgcatt tccgcaattg catcgttcac tgctttcagc aggtcttcat
     2761 ttcctttacg cagcgccacg ccagactcct gacgggagaa tgcttcaccg gttactgcca
     2821 gcgtatcgtt ggttttcttc accagatcca gcgccgccag acggtcaacg aggatcgcat
     2881 cgatacggcc tacgcgcaga tcctgatatt tggtcgggtc atcatcatag gtacgcacat
     2941 cgacgccctg aacattctgc cgcagccact cttcatagtt ggtgcccaga ccgacaccca
     3001 cttttttgcc tttcagatca gcagccgttt taatggtgcc ttcgttacct tttttcacca
     3061 gcgcctgaat accagaaatg gtgtacgggg tagagaaatc gtattttttc ttgcgctcat
     3121 cagaaatggt gacctgatta atcaccacat caatacgttt agagtccagc gacgctagca
     3181 taccgtccca tttggtcggt tttagtgacg cctcaacgcc aagatgtttt gccagctgtt
     3241 gggcaaattc cacttcaaaa ccggttaatt tgccgtcatc cccctgaaaa ctgaacggcg
     3301 gataagttcc ttccagccct accagtagcg taccgcgctc tttgacttta ttaagcagac
     3361 cttcatctgc aaaactttta acgctcatgc ccgcaaccag cgccacggcc atcacaccca
     3421 tcaatgcctg acgtcccaga tgtgctaatt tcatattcac cccgaatgtt gttatgtctg
     3481 tttgcagtgt agagccattg tttgtaaaca caaaaacaac tccgctacat cttattctta
     3541 tttaatatat atcagaagca ggcaggctgg aggatttctg cacaagtccg gtacacgttt
     3601 gacgctgata gtgttgatac ttccgtaatg cgatgcggta attgttggtg ctggtggtgg
     3661 gcagccacgg ggcgaggatt tcatcaagga aaccgtcctg cagctgatcg agggaaatat
     3721 tttgctcatg tagatgattc cccaaacggc gcaaacgaac gacatattca cgcaccgtac
     3781 ctgggctcat ttcagtttgc tcaaaaagaa attgcttaaa gccgataata tcgaaaaaat
     3841 cactctgcgt tttgcaatgt aaatcaccgc aaaatcggca caatgccgcc catgactgtt
     3901 gttccacttg ccagccattt tcatcaagca gcgtgtccag gcgggaaatc tcgattttat
     3961 tcactatttt gccgtcgcga actaaggtaa tgcgatcgag taatttacgg caatgcgcgc
     4021 aatgggcctg gctgtgttta aagtctttaa gatagcggct taatggccgt cttttcaggt
     4081 gctgcaccat cattaagaac tcctggtagt caaagttaaa gtgcggcatt tactgacgtt
     4141 ataacttacc cagtttagtg cttaaccgtt taatggcctg gctgtgtaac tgactgaccc
     4201 gcgattcccc gacctccagc accgcgccaa tctctttgag attcagctct tcctggtaat
     4261 agagggttaa taccagtttt tcgcgctccg gcaacgtttc gatagcttcc atcacccgct
     4321 ggcgcagatt actgtccagc agttgttgta gcgggttttc tcgctgatga tcatcagtaa
     4381 ccagttcgat gctatcgccg tgctcttcgc gccactcatc gtaggagaaa agctggctgt
     4441 tattggtgtc gagcaacatt tggcgataat cggcaatatc gatccctaaa cgttccgcta
     4501 cctcagtttc cgtggcgttg cggccaagtt cctgctccag ttgccctatt gcctgtgcca
     4561 cttcacgcgc gttgcgtcgc acgctgcgcg gcacccagtc acggctgcga agttcatcca
     4621 gcatagcgcc acggatacgc tgcactgcgt aagttgtaaa tgccgttcct tgtagggcgt
     4681 catagcgttc gacggcatta agtaacccaa tgccgcccgc ctgtagcaga tcgtcaagtt
     4741 ccacgctcgc gggcagtcga acctgcaggc gcaatgcttc gtgacgcacc agcgggacat
     4801 aacgctgcca cagcgagtgt ttatccatta caccttcagc ggtatagagt gaattcacga
     4861 taaacagccc tgcgttatat gagttatcgg catgattatc cgtttctgca gggtttttaa
     4921 tcggacgatt agtgggtgaa atgaggggtt atttgggggt taccggtaaa ttccatgcag
     4981 aaaaaacccc gccggtggcg gggaagcacg ttgctgacaa attgcgcttt atgttgccgg
     5041 atgcggcgta aacgccttat tcggcctaca aaaatgtgca aattcaataa attgcaattc
     5101 accttgtagg cctgataagt gcagcgcatc aggcaatttg gcgttgccgt cagtctcagt
     5161 taatcaggtt acgacgatta accctgcagc agagacagaa cctgctgcgg tacctggttg
     5221 gcttttgcca gcacggagtt accggcctgc tggatgatct gcgctttcga catgttggac
     5281 acttcggtcg catagtcggc gtcctgaata cgggactgcg cttcagacag gttggtagtg
     5341 gtgttgttca ggttggtgac tgcagaatcc agacggttct gaatagcacc cagggatgaa
     5401 cggaatttgt cgatggagct gatagcttca tccagggcag ccagaggatc tttggttgca
     5461 gtgcctgcac tggtggtttc agtggtcagt ttgcctgtag agttaacata agcctgtgcc
     5521 ccaacagtag ctgcaaattt accagttgca cctgcgccat ttgaagctac agttactgta
     5581 ccggtatcct tatcgacgtt gtagttgata gtgtaagatg cggttgtggt gagctctttg
     5641 tctgcatcag tataggtgtc attcgaatta ccagcaccat tagtattcgc accaccgaag
     5701 gacgccgttg cagactgaat acctgtatta tacttaattg tcgcaccagt aactgctgtg
     5761 ttagccgttg cagcactctg cactgtgctc agtacagtat cagcgcttac agttttggtg
     5821 taggttacgc cagaagttgc cgctgcacca aagttataag ttgtgccaga caactggata
     5881 gaaccagttg tcgctaaggt actaccgcta gcaaacagat cggacaattt cgctgaagat
     5941 gcaatgccgg cgttgtttgt agataggtta ccagtggcgt ccaggtacgc ggctttacca
     6001 ccgatggtca catcgccatt agcatctaca tcaaacttca catcaccagt actacgagta
     6061 taaacacctg atgcagtagt gccagccgct ggtttcagag tattcgcaaa gttaacaaca
     6121 tcgccatctg caactgttgc ttgagtggtg aagttccctt tagccgcatc ataagtatag
     6181 atcgcagcac tcgagccagt agtagtaact gtatctccgg ttttcaggcg agacagtgca
     6241 tcgctagcgc tgagtgctgt attgtttgtg gtcacagcat aaggacctgt tcccgttgca
     6301 ccagcagcgg tcagatcgct gactgtagca gctttgttcg caatagtgcc tttgccattc
     6361 acgttaaaac cattcagccc cagcgtatct gagtcaattt tcttcagatc aatcgtgata
     6421 gtctggccgt cattcgcacc aacctgaatt ttcattgaac cgtctttcgc cagtacgttc
     6481 acgccgttga actgggtctg gccagatacg cggtcaattt cgtccagacg ggatttgatt
     6541 tcgtcctgaa tggagtccag atccgaatcg gagttagtcc cggtagaagc ctgaaccgtc
     6601 agttcaagaa tacgctgtaa gttgttgttg atttcggaca gcgcgccttc agtggtctgc
     6661 gcaacggaga taccgtcgtt ggcgttacgg gccgcctgag tcaggccttt aatattagaa
     6721 gtaaaacggt tagcaatcgc ctgacctgcg gcgtcatcct tcgcgctgtt aatacgcaag
     6781 ccagaagaca gacgctcgat agaactcgac agcgcagact ggttcttgtt gatattattt
     6841 tgagtgatca gcgagaggct gttggtatta atgacttgtg ccatgattcg ttatcctata
     6901 ttgcaagtcg ttgattacgt attgggtttc cacccgtcgg ctcaatcgcc gtcaaccctg
     6961 ttatcgtctg tcgtaaaaca acctttagaa tttttttcac aaacagccat tttttgttag
     7021 tcgccgaaat actcttttct ctgcccctta ttcccgctat taaaaaaaac aattaaacgt
     7081 aaactttgcg caattcaggc caataacccc ggtattcgtt ttacgtgtcg aaagataaaa
     7141 ggaaatcgca tggcaagtat ttcatcgctg ggagtcgggt caggtctgga tttaagttca
     7201 atccttgata gcctcaccgc cgcgcaaaaa gcgacgctaa cccctatttc aaatcagcaa
     7261 tcgtcgttta ccgctaaact tagcgcctac ggtacgctga aaagcgcgct gacgactttc
     7321 cagaccgcca atactgcatt gtctaaagcc gatctttttt ccgccaccag cactaccagc
     7381 agcaccaccg cgttcagtgc caccactgcg ggtaacgcca tcgccgggaa atacaccatc
     7441 agcgtcaccc atctggcgca ggcgcaaacc ctgaccacgc gcaccaccag agatgatacg
     7501 aaaacggcga tcgccaccag cgacagcaaa ctcaccattc aacaaggcgg cgacaaagat
     7561 ccgattacca ttgatatcag cgcggctaac tcatcgttaa gcgggatccg tgatgctatc
     7621 aacaacgcaa aagcgggcgt aagtgcgagc atcattaacg tgggtaacgg tgaatatcgt
     7681 ctgtcagtca catcaaatga caccggcctt gataatgcga tgacactctc ggtcagcggt
     7741 gatgatgcgc tacaaagttt tatgggctat gacgccagtg ccagcagcaa cggtatggag
     7801 gtctcggtcg ccgccaagaa tgcgcagctg acggtcaaca acgtcgccat tgagaacagc
     7861 agcaacacca tcagcgacgc gctggaaaac atcaccctga acctgaacga tgtcaccacg
     7921 ggcaaccaga cgctaaccat cactcaggac acctccaaag cgcaaacggc gattaaagac
     7981 tgggtgaatg cctataactc gttaatagat accttcagta gcctgaccaa atacaccgcc
     8041 gtagatgcgg gagctgatag ccagagttct agcaatggcg cactgctcgg cgactccacg
     8101 ctgcggacga ttcagacgca gttgaagtcg atgctgagta ataccgtcag ttcttccagc
     8161 tataaaacgc tggcgcagat tggtatcacg accgatccca gcgatggcaa actggaactg
     8221 gatgccgaca aactcaccgc tgcactgaaa aaagatgcca gcggcgtagg tgcattgatt
     8281 gttggcgatg gtaaaaaaac cggcatcacg accaccatcg gcagcaacct gaccagttgg
     8341 ctttcgacaa cgggcattat taaagccgct accgatggcg ttagtaagac cctgaataag
     8401 ttaactaaag actacaacgc cgccagcgat cgcattgatg cgcaggtcgc gcgctacaaa
     8461 aaacaattta cccaactgga cgttttaatg acctcgttaa acagcaccag cagctactta
     8521 acgcagcagt tcgaaaacaa cagtaattcc aagtaatcat tattcatcgg gagacaggtc
     8581 atgtacgcgg caaaaggcac ccaggcctat gcacaaattg gcgtcgaaag cgccgtaatg
     8641 agcgccagcc agcagcagct ggtcaccatg ctatttgatg gagtgctgag cgcactggtt
     8701 agagcgcgcc tgtttatgca ggacaacaat cagcaaggca aaggcgtctc tttgtcaaaa
     8761 gcgatcaaca tcattgagaa cggactgcgg gtgagtcttg atgaagagag caaagacgaa
     8821 ctaacccaaa acttgattgc tctttatagc tatatggtca ggcgcttgct gcaagccaat
     8881 ttacgcaacg atgtctccgc agtcgaagaa gtggaagcat taatgcgcaa tattgccgat
     8941 gcctggaaag agtcgttact ctccccttct ttgattcagg acccagtctg atgaaccatg
     9001 caccgcattt gtatttcgcc tggcaacaac tcgtcgaaaa aagccagctc atgttacgcc
     9061 tggcaacgga agaacaatgg gacgaactca tcgccagcga aatggcgtat gtgaatgcgg
     9121 tgcaggagta tagcacacct gactgaagag gttgccccgt ccaccacgat gcaggagcag
     9181 ctccgcccga tgctgcgcct gattctcgac aacgaaagca aggtaaagca gttattacag
     9241 attcgtatgg atgaactggc gaaactggtc ggtcagtcat cggtgcaaaa atcggtgtta
     9301 agtgcctatg gcgatcaggg cggctttgtg ctggctccgc aagataactt ctcgtgaatc
     9361 ttaacgagct aatggttcgc gaataatccg attacggcta cgcttctaat gttccccttg
     9421 aatggagtcg aagaatgcgt aatcccacgc tgttacaatg ttttcactgg tattacccgg
     9481 aaggcggtaa gctctggcct gaactggccg agcgcgccga cggttttaat gatattggta
     9541 tcaatatggt ctggttgccg cccgcctata aaggcgcatc gggcgggtat tcggtcggct
     9601 acgactccta taatttattt gatttaggcg agtttgatca gaaaggcagc atccctacta
     9661 aatatggcga taaagtacaa ttgctggccg ccattgacgc cctgaaacgt aatgacattg
     9721 cggtgctgtt ggacgtggta gtcaaccaca aaatgggcgc ggatgaaaaa gaagctattc
     9781 gcgtgcagcg tgtaaatgct gacgaccgta cgcaaattga tgaagaaatc attgagtgcg
     9841 aaggctggac gcgttacacc ttccccgccc gtgccgggca atactcacag tttatctggg
     9901 atttcaaatg ttttagcggt atcgaccata tcgaaaaccc tgatgaagat ggcatattta
     9961 aaattgttaa cgactacacc ggcgaaggct ggaacgatca ggttgatgat gaattaggta
    10021 atttcgatta tctgatgggc gagaatatcg attttcgcaa tcatgccgtg acggaagaga
    10081 ttaaatactg ggcgcgctgg gtgatggaac aaacgcaatg cgacggtttt cgtcttgatg
    10141 cggtcaaaca tattccggcg tggttttata aagagtggat cgaacacgta caggaagttg
    10201 cgccaaagcc gctgtttatt gtggcggagt actggtcgca tgaagtcgat aaactgcaaa
    10261 cgtatattga tcaggtggaa ggcaaaacca tgctgttcga tgcgccgctg cagatgaaat
    10321 tccatgaagc atcgcgcatg gggcgcgact acgacatgac gcagattttc acgggtacat
    10381 tagtggaagc cgatcctttc cacgccgtga cgctcgttgc caatcacgac acccaaccgt
    10441 tgcaagccct cgaagcgccg gtcgaaccgt ggtttaaacc gctggcgtat gccttaattt
    10501 tgttgcggga aaatggcgtt ccttcggtat tctatccgga cctctacggt gcgcattacg
    10561 aagatgtcgg tggtgacggg caaacctatc cgatagatat gccaataatc gaacagcttg
    10621 atgagttaat tctcgcccgt cagcgtttcg cccacggtgt acagacgtta tttttcgacc
    10681 atccgaactg cattgccttt agccgcagtg gcaccgacga atatcccggc tgcgtggtgg
    10741 tcatgtcgaa cggggatgat ggcgaaaaaa ccattcatct gggagagaat tacggcaata
    10801 aaacctggcg tgattttctg gggaaccggc aagagagcgt aataaccgac gaacacggcg
    10861 aagcaacctt cttttgcaac ggcggcagcg tcagcgtgtg ggttatcgaa gaggtgattt
    10921 aaattcatcc ccggcggcaa gccggggaga tttcattacg gcagtggcgt cggtaacggc
    10981 gctttatcca gcgcggcggc acactctgcc gtcgggcgat ccacacgctc catcgtcata
    11041 ccatcgtact caatggtgtt accgtcacgc tcaacctcgt acagctcacg ttttacggta
    11101 acgttagtga gatcatcaga catcaatgcc aacttacccg gcacagcaat cacccgctgc
    11161 cactgacggc aatccagagt atcaccctct ttagtgacaa tcaggctgcc tatcgcttcc
    11221 ggactgacca gcgcacgttg cggccctttg gtctgccagt agcccgccag ccagtccggc
    11281 gcgggggttt tcacgacatt gttatagttt tcgacttcgg cacagcccgc cagcaacatg
    11341 agcgcgcctg caattgcgag ttttttcatc attcgtcctg catgagaaga aaagaggatt
    11401 gtggcattaa agccctgatg tcgccagcct ttccaggttc agagagaaat ttgatctaac
    11461 tattattttt gtgaattatt accccgaaaa taaatttgca tgatgtatga ttcgcgactc
    11521 ttgtttcaaa ccttctgagt tcagaggcta cattcatgtc atggcagcaa ttcaaacacg
    11581 cctggttgat taaattctgg gcacccatcc ctgcggtcat cgcggcgggt attctctcca
    11641 cttactattt tggcattact ggcacctttt gggctgtcac gggtgaattt acccgttggg
    11701 gcggccagct cctgcagctg tttggcgtcc atgctgaaga gtggggttat tttaaaatta
    11761 tccatctgga aggatcgcca ttaacccgca tcgacgggat gatgatcctc ggcatgtttg
    11821 gcggctgctt tgccgcagcg ctgtgggcca acaatgtcaa actgcgcatg ccacgcagcc
    11881 gtatccgcat tatgcaggcc attattggcg gtattatcgc tggttttggc gcgcgtctgg
    11941 caatgggctg taacctggcg gcgttcttta ccggtattcc tcagttctcg ctgcatgcct
    12001 ggttctttgc catcgccact gccattggtt catggtttgg cgcacgcttt accctgctgc
    12061 ccatcttccg tattcccgtg aaaatgcaga aagtttctgc cgcctcaccg ctgacgcaaa
    12121 aaccggatca ggcgcggcgt cgttttcgtc tcgggatgct ggtctttttc ggtatgctgg
    12181 gctgggcgct gctcacggcg atgaaccaac caaaactggg gctggcaatg ctgtttggcg
    12241 tcggctttgg tttactgatt gaacgtgcgc aaatctgctt tacttcggcg ttccgcgata
    12301 tgtggatcac cggacgtacc catatggcga aagcaatcat tatcggtatg gcggtgagtg
    12361 ccatcgggat cttcagttac gtacagttag gcgttgaacc caaaatcatg tgggcgggac
    12421 caaacgcggt aattggtggt ttactgtttg gttttggcat cgtgctggca ggcggctgcg
    12481 aaaccggctg gatgtaccgc gcggtagaag gccaggtgca ctactggtgg gtcggtctgg
    12541 gcaatgtgat cggctcgacg attctggcgt attactggga tgatttcgct ccggcgctgg
    12601 ccaccgactg ggacaaaatc aacctgctga aaacctttgg cccgatgggt ggcctgctgg
    12661 tgacatattt gctgttgttt actgcgctga tgttgattat cggctgggaa aaacgcttct
    12721 tccgccgtgc ggcaccgcag actgctaagg agatcgcatg aaaaatatcg ttcctgatta
    12781 ccgccttgat atggtgggtg aaccctgccc ttatccggca gtcgcaaccc ttgaggcgat
    12841 gccgcagtta aaaaaagggg aaatcctgga agtggtgagc gactgtccac agtcgatcaa
    12901 taatattcca ctggatgcgc gtaatcacgg ctatacggtg cgggatattc agcaagacgg
    12961 gccgaccatt cgttatttaa ttcagaagta atccctcatt cccgctgtgg cccacagcgg
    13021 gaacatcgtt gcctctgaca catagatttt acccgccccc tctcctattc ttttcagtat
    13081 gatctcagga ggtttctatg tgtggacgct ttgcccaatc ccaaacgcgt gaagattacc
    13141 ttgcgcttct cgcggaagat attgaacgcg atattcccta cgatcccgaa cccattggca
    13201 gatacaacgt cgcgccggga accaaagttc ttctgctcag tgaacgtgat gaacaccttc
    13261 atctggatcc ggttttctgg ggatatgctc ccggatggtg ggataaaccg ccgctgatta
    13321 acgcccgcgt agaaactgcc gcaaccagtc gcatgtttaa accgctctgg caacatggtc
    13381 gggcaatctg ttttgccgat ggctggtttg agtggaaaaa agaaggcgac aaaaaacagc
    13441 ctttttttat ctatcgtgct gatggacaac ctatttttat ggccgcgata ggtagcacac
    13501 catttgagca tggtgacgaa gccgaaggat ttttaattgt cactgctgca gcagaccagg
    13561 gtctggtaga cattcatgac cgccgcccgc tggttctgtc gccagaagct gcacaggaat
    13621 ggatgcggca agagattggc ggtaaagaag catcagaaat tgctaccaac ggctgtgtgc
    13681 cagcaaacca gttcacctgg cacccggtat cgcgcgcggt aggtaatgtc aaaaaccagg
    13741 gcgcggagtt aattcaacct gtttgttgac atcctccacg ccctgaagga cgaggctccc
    13801 tgctacgttc aggctgtcgc ctgaatcatt tcggtgggtt cctgcttcaa cgggcggcct
    13861 gactgcacca tctctccaca ggcaagaacg gcgtgccccg ccgctaaaat gttacgagcg
    13921 ccatttacat cggcgttcgc tgtatatcca catacctggc atctgaattt actttgtgac
    13981 aggcggtttt ctttcgctgt atgaccacag cacgcgcaac gctggcttgt gcacgctggc
    14041 ggaacagcaa acacctgacc gccacgccag agctgcttgt actcaagctg gcggcgcatt
    14101 tcataccagc cctgatccag tatcgaacgg tttaaacctg attttgcccg gacattgcgc
    14161 cccggctggc tgaccgtacc cgctgctgac tttgacatgt tgctgacctt caaatcctca
    14221 atgactatca ttgcgtggtt tttgctgacg gtcgttgtga ctttgtgaag gtagtccctg
    14281 cggatatttg cgatacagga atgcagtcgc tgtattttgc gtttctgctt ctgccagttg
    14341 ttgctgaatt tgaccttgcg gcttaactgt cgctgaagtc tcgccagcgt cttctggttt
    14401 ttctgaaaac tgtttacagg ctcaaaggct gtgccatctg acagcgtggc gagtttagcc
    14461 acgccagcat ccagcccgac cattgatgct gaagggtgaa ccggagttga tacttcactt
    14521 tctgtctgaa tactgatgta ccacttaccg caggactggc tgacagtgac atttttcaca
    14581 acacccgtga cctgacggct gttccggtag cgcatccagc ccagtttcgg cagaaaaata
    14641 cggctgtttt cctgatcgag cttaacgccc tgcgggtagc ggaatacatc attctgtccc
    14701 cgctttttga atcggggaaa agcagcccga ttctggaaga agtttttgta agcccgctca
    14761 aggtctttca gtgactgttg caatggctgt gacggggcat ctttaagcca ttgcgtttca
    14821 gtggcatttt tccactcaac cagccaggaa gccattttcc cgtaagggat gtatttatta
    14881 ccgacctcat gattctcatt ctgacgtgcc agagcacgat tgaaaacgaa acgacatgcg
    14941 cctgcgaagc gcctcatttc acgctcctgt tgaccaccgg gtcttaactg gaatttaaat
    15001 gcttgtagtc gtttcacagg ccaaatgata ctttggtctg tgaaaaaaga aaccgatatc
    15061 cggcgtggaa ggcattgtgt tttcctgatg catgttcacc tggtctttgt caccaaatac
    15121 cgacgtcaga tttttgacca tgacgcgaca gaaaaactac gcacttactt ttcaaatgta
    15181 tgtgctgatt ttgaagctga actggttgaa atggatggcg aaccagatca cgtccatttg
    15241 ttaattaact atcctcccaa actggcaata tccagtctgg taaacagcct caaaggcgtt
    15301 ttgtggagtc ctggctattt tgccagtagc tgcggaggtg cgccaatatc cgtcatccgc
    15361 caatacattg aacaacagca aacacctggt taggtggaaa accacgcctt atatccctgc
    15421 cctgaaggac ggggttttac ggcgcaccgg ataagcctgg agattatcta caccagcatg
    15481 gacatcattg tactatgccc cgtacatgcc ccatcacatc ctcaacatca aagtattaga
    15541 gccttgcaaa ccattatgcg tctcagtttg tctcatctat cttacgcaaa aaaaaataga
    15601 actaatatca agc
//
LOCUS       DAZPEB010000092        15425 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZPEB010000092 DAZPEB010000000
VERSION     DAZPEB010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15425
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.093"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(476..1672)
                     /gene="hemY"
                     /locus_tag="ACHURJ_002439"
     CDS             complement(476..1672)
                     /gene="hemY"
                     /locus_tag="ACHURJ_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFR1281347.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1675..2880)
                     /gene="hemX"
                     /locus_tag="ACHURJ_002440"
     CDS             complement(1675..2880)
                     /gene="hemX"
                     /locus_tag="ACHURJ_002440"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFR1281348.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPQGD
                     TPAAAPQGE"
     gene            complement(2902..3642)
                     /gene="hemD"
                     /locus_tag="ACHURJ_002441"
     CDS             complement(2902..3642)
                     /gene="hemD"
                     /locus_tag="ACHURJ_002441"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFR1281349.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLRCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3639..4580)
                     /gene="hemC"
                     /locus_tag="ACHURJ_002442"
     CDS             complement(3639..4580)
                     /gene="hemC"
                     /locus_tag="ACHURJ_002442"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFR1281350.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDARTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4967..7513
                     /gene="cyaA"
                     /locus_tag="ACHURJ_002443"
     CDS             4967..7513
                     /gene="cyaA"
                     /locus_tag="ACHURJ_002443"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFR1281351.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            complement(7553..7873)
                     /gene="cyaY"
                     /locus_tag="ACHURJ_002444"
     CDS             complement(7553..7873)
                     /gene="cyaY"
                     /locus_tag="ACHURJ_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFR1281352.1"
                     /translation="MNDSEFHRLADQLWLTIEEHLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8035..8177)
                     /locus_tag="ACHURJ_002445"
                     /pseudo
     CDS             complement(8035..8177)
                     /locus_tag="ACHURJ_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            8336..8538
                     /locus_tag="ACHURJ_002446"
                     /pseudo
     CDS             8336..8538
                     /locus_tag="ACHURJ_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
     gene            8575..9399
                     /gene="dapF"
                     /locus_tag="ACHURJ_002447"
     CDS             8575..9399
                     /gene="dapF"
                     /locus_tag="ACHURJ_002447"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFR1281353.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            9396..10103
                     /locus_tag="ACHURJ_002448"
     CDS             9396..10103
                     /locus_tag="ACHURJ_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HFR1281354.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            10100..10996
                     /gene="xerC"
                     /locus_tag="ACHURJ_002449"
     CDS             10100..10996
                     /gene="xerC"
                     /locus_tag="ACHURJ_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFR1281355.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            10996..11712
                     /gene="yigB"
                     /locus_tag="ACHURJ_002450"
     CDS             10996..11712
                     /gene="yigB"
                     /locus_tag="ACHURJ_002450"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFR1281356.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            11796..13958
                     /gene="uvrD"
                     /locus_tag="ACHURJ_002451"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             11796..13958
                     /gene="uvrD"
                     /locus_tag="ACHURJ_002451"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFR1281357.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCLPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(14003..14905)
                     /locus_tag="ACHURJ_002452"
     CDS             complement(14003..14905)
                     /locus_tag="ACHURJ_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HFR1281358.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKSGQYFVSPDNGTLTLVAQTLGIDSVREIDEKANRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAEIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(14988..>15425)
                     /locus_tag="ACHURJ_002453"
     CDS             complement(14988..>15425)
                     /locus_tag="ACHURJ_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFR1281359.1"
                     /translation="QKVALNLASGEGNFFIRPGGVFYVAGDKVGIIRLDAFKTSKEIQ
                     FAVQSGPMLLENGVINPRIHPNVASRKIRNGVGINKQGNAVFLLSQQATNFYDFACYA
                     KAKLNVEQLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
BASE COUNT         3497 a         4044 c         4138 g         3746 t
ORIGIN      
        1 tcgcgttgat tattgatgct ttttttagtt ttaacggcaa ttaatatatg tgttattaat
       61 tgaatgaatt ttatcattca taataagtat gtgtaggatc aagctcaggt taaatattca
      121 ctcaggaagt tattactcag gaagcaaaga ggattacaga attatctcat aacaagtgtt
      181 aagggatgtt atttcccgat tctctgtggc ataataaacg agtagatgct cattccatct
      241 cttatgttcg ccttagtgcc tcataaactc cggaatgacg cagagccgtt tacggtgctt
      301 atcgtccact gacagatgtc gcttatgcct catcagacac catggacaca acgttgagtg
      361 aagcacccac ttgttgtcat acagacctgt tttaacgcct gctccgtaat aagagcaggc
      421 gtttttttat gcatcagaaa ggtcccggag gtgcttgcct ccgggtgaga aggaactact
      481 gtggtgggtt gttctgcaac gttaacatca aaccgtcgcg acgcatcgct gcagcttctt
      541 ccggcttgtg cagtctgtcc agcgcgtcgg caagccatgc gtaatcgtag gcgtccggac
      601 gttgtttcag cgctgcgcgg aaggcgagcg atgcttcctg ccattctccg tgcttcatca
      661 gtgactggcc cagtgtgctc cacaacagcg ggcgatcgcc gacgtttttg atctgctggc
      721 gcagcacttt ttccagctgt tccggattgt tggttttcag tcgaggaatc ggcagcagta
      781 ggcgatcgtc gtactggcgt ttcaggccat cgatgataat ttgctgggca gtatcatgat
      841 cgtcacattc aataagatgt tccgccattg ccacctgcaa cgctacttga tgacgcgttt
      901 tccggctttg gtttttccac cagttacgca aaccttcact accgttatcg gcacgcgcct
      961 gatccatcag gccaatccat gcctgttgtt ccagcattgc acgatgttct tcatcaccaa
     1021 catgggcttt cgccattgat gggatgatat ccagcagaga actccatgca cctgtgcgga
     1081 tatacgcctg ttccgccaga cgtaatactt ccggatggcg tggcgtaact tccagcagct
     1141 tatccacgcc gtggcgtgca gcatggtttt cattacgggc cagttgcaga cgtacacggg
     1201 tgatttctac cggaatggtg tcattgccgg ccagctccgc tgcgcgttcc agatgttggt
     1261 tggcgcgcgc ttcatcacca cgttgttgcg cggcttcggc agccagtaga tagttcacca
     1321 ccggttgttc agcgtgatcg gcatttttcg ccatcagctt ttcaacttgc tgataatcgc
     1381 cttccgccag tttcagcagc gcctgttcgg tctgcttacg tgcacggcga cgcttacgcc
     1441 cgacaaacca cccacgggtg tgcgcgccag tgcggaagat ccgccgcagt agccactcaa
     1501 tggcaaacag cactaccatc gccagaatca atatgatcgc caggcccgtg acgctggttt
     1561 cgatattgta gttgtcggtc tggatcagca cataaccctg atgaccggca atcatcgggc
     1621 caaccactat ccccgcaatc agcaaaacaa agagtaataa cacttttagc atggttattc
     1681 tccttgcggc gcggctgccg gagtatcacc ttgcggtgca ggtgcaggtg caggtgcagg
     1741 tgcaggtgca ggtttagctt ccgttgtccc tgctgccggt tgtgccagca ggttacgcac
     1801 gcgagtctgc atcagttttt ccagcatcgc ctggctttgc agggtttccg gaagatccat
     1861 cgagatattt tgctggctta actggtccac ctcgtcgagg aacgctttgg tggtggcatc
     1921 atcagtatcg tagtaagcac gtacccaggt ggagacgttc tccagcgcct ggcgataagt
     1981 ctcttcctgg tgacgcggta cagcttgtgc tgcgaccagc aggcgagagc gaatattttc
     2041 gcgcagatag atatcctgat ttggcgctaa cagcggtacg gcggtgtcat cacgacggcg
     2101 aatcgtaatg aagttgtcca taaagttctg ccagcttttt tgcagattga tacgccattc
     2161 gctgatggaa ctggaaagct cttcaccatc ggaatccatc ggcgaaccat cgctgtcatt
     2221 atcggccaga cgcaggttat ctacctgatt tgaaagctga ttaagcttaa ggatgatgcc
     2281 gtcataatcc acctgcgaaa ctgcagaaag gctggcgata tcatcggtaa ttgcccgacg
     2341 aacggtaatc agactcgggt cattcatatc cgccaggctg gcatctgcac ttttcagcaa
     2401 cgctgcagcg gtcgtgacgt cctgatcgct ccacagcttc cgtccggcga gtttcaccag
     2461 aaaatcggcc tgagccagca gccaggtttt agcatcgctg ccggaaatgg tggcgacctt
     2521 ttgttggact tcatccaact gttttgccag cgtttcttgc tgacgattcg cctgcttaag
     2581 ctgtgcagct tgttgcttaa taatgccttc cagctcggct ttttggctct cctgggcttt
     2641 ttgcaatgcc gtcagttggt tagccagggc atcgctggtg gcggtctgat tgacggcctg
     2701 ttgtttaccc cagccataca aaccgacgcc cgccgccaga gcaatagcga tagccaccgc
     2761 gctgagaatc aatgcggtat tgttcttact ctttttttct gttgcgacag gttgtgacgt
     2821 ggtgtccacg gcctccctgg tctcttcaac cacggcggag gttttttctt gttccgtcat
     2881 tatggcttcc tgttatgaga gttattgtaa tgcccgtaaa agcgcatcgt tgtcagcgtt
     2941 atcggcgacc ttaatgtctt gccagcccag ttcccgggcg agtttcgcca aacgctcact
     3001 gacgaccaat agtcgacagc gtagtaacca gtgctcacga taccattgtg ggatcagcga
     3061 ccagagttgc tgcaacattt caccgctggt aacaacgacc atcgtcacct cgcgggattg
     3121 ccagcgcatc gcttcttctg caccatcgta atggattgcg catcgttgat aacattcaca
     3181 aaaagtgacc tcagcaccgc gcgctgtcag ggtatcccca attagctcac gaccgccatt
     3241 gccacgtaat atcagcgcac gtttgcccgc aatattttgt aattcaggta attgtagcaa
     3301 gacttcgctg atttcccgat cctgcgggta gagaatcttc tgtccgctta cggtatgtag
     3361 cgccagtgcg gtggtgcgtc caatggcgaa ataatcaggt agtcggggcc atttacgatc
     3421 ttgctgatgc agctgtgatt gggcaaaagc aaccgcgtgt tgcgagaggg caaacaacag
     3481 atcgctctcc cccagcgccg ccagttgatc agcaagttgc ggtaattgtc gacccggaga
     3541 aaactcaatc agcggaaaat gccaggccac ctgccccagt gtgcgcagac ggctcactaa
     3601 ctcttctcca gcgggagacg ggcgggtgac caggatactc atgccggggc gtctccgtta
     3661 tagacttcag cgaggatctc gcgcgcgccg ttattcagta gctcttctgc cagcgaaatc
     3721 cccatttgtt cggcatcttg cggcgcaccg cggcgttcac cgcgaataat ctgcgaaccg
     3781 tccggcgcgc cgaccagcgc acgcagccag atttcaccat caataagctc ggcgtagcta
     3841 ccaattggca cctgacatcc gccttcgaga cgggtattca tggcgcgttc tgcggtaacg
     3901 cgcagtgcag tttcgtggtg attcagcgcg gcaagcagct cgcgagtgcg tgcatcatca
     3961 aggcggcatt caataccgac cgcaccttgt cctaccgctg gaagagaaat ctcgggtggt
     4021 aacgcggcgc gaatgcggga ctccagacct aaacgtttta gtccggctac ggcaagaatg
     4081 atggcatcgt attcgccgtt atctagtttg ctcaggcgag tgccgacgtt gccgcgcagg
     4141 gagcggataa tcagatccgg gcggcgttca gccagttggc actggcgacg taaactggac
     4201 gtcccgacga tactgcctgc tggtaacgca tccagactgt catagttatt ggacacaaag
     4261 gcatcgcgag gatcttcacg ctcacaaata gtgaccagtc ccagaccttg cgggaattca
     4321 accggcacat ctttcattga atgtacggcg atatcggcgc gattttcgag gagcgcgact
     4381 tccagctctt ttacaaataa gccttttccg cctactttcg ccagcggcgt atcaagaatc
     4441 acatcgccgc gcgtcaccat cggtaccagt tcaacgacca ggcccggatg gctcgccatc
     4501 aacttgtctt tgacatagtg tgcctgccag agtgcaagtg ggctttggcg tgtggcaatt
     4561 cttaaaacat tgtctaacat gcttgttacc gtcattatca tccgtggtcc atcctaacat
     4621 ccttgccaga gtgatgtcag tgttgtggtg aaacgtagac gcctgcgcaa accgtaaaat
     4681 gaggtctggc agtggatcct gacaggcgtt tcacgccgtc gtaataagga atttacagag
     4741 aataaacggt gctacacttg tatgtagcgc atctttcttt acggtcaatc agcaaggtgt
     4801 taaattgatc acgttttaga ccattttttc gtcgtgaaac taaaaaaacc aggcgcgaaa
     4861 agtggtaacg gttacctttg acatacgaaa tatcccgaat gccgcgtgtt accgttgatg
     4921 ttggcggaat cacagtcatg acgggtagca aatcaggcga tacgtcttgt acctctatat
     4981 tgagactctg aaacagagac tggatgccat aaatcaattg cgtgtggatc gcgcgcttgc
     5041 tgctatgggg cctgcattcc aacaggtcta cagtctactg ccgacattgt tgcactatca
     5101 ccatccgcta atgccgggtt accttgatgg taacgttccc aaaggcattt gcctttacac
     5161 gcctgatgaa actcaacgcc actacctgaa cgagcttgaa ctgtatcgtg gaatgtcagt
     5221 acaggatcca ccgaaaggtg agcttccaat tactggtgta tacaccatgg gcagcacctc
     5281 gtccgtaggg caaagttgtt cctctgacct ggatatctgg gtctgtcatc aatcctggct
     5341 cgatagcgaa gagcgccagt tactacaacg taaatgtagt ctgctggaaa gctgggccgc
     5401 ctcgctgggt gtggaagtca gcttcttcct gattgatgaa aaccgcttcc gtcataatga
     5461 aagcggcagc ttggggggcg aagattgtgg ctccacccag catatactgc tgcttgacga
     5521 attttatcgt accgccgtgc gtctcgccgg taagcgtatt ctgtggaata tggtgccgtg
     5581 cgacgaagaa gagcattacg acgactatgt gatgacgctt tacgcgcagg gcgtgctgac
     5641 gccaaatgaa tggctggatc tcggtggctt aagctcgctt tctgctgaag agtactttgg
     5701 tgccagcctt tggcagctct acaagagtat cgattcccca tacaaagcgg tactgaaaac
     5761 actgctgctg gaagcctatt cctgggaata cccgaaccca cgtctgctgg cgaaagatat
     5821 caaacagcgt ttgcacgacg gtgagattgt atcgtttggt ctcgatccat actgcatgat
     5881 gctggagcgt gttactgaat acctgacggc gattgaagat tttacccgtc tggatttagt
     5941 acgtcgctgc ttctatttaa aagtgtgcga aaagctcagc cgtgaacgcg cctgcgtagg
     6001 ctggcgtcgc gcagtgttga gccagttagt gagcgagtgg ggttgggacg aagctcgtct
     6061 ggcaatgctc gataaccgcg ctaactggaa gattgatcag gtgcgtgagg cgcacaacga
     6121 gttgctcgac gcgatgatgc agagctaccg taatctgatc cgctttgcgc gtcgcaataa
     6181 ccttagcgtc tccgccagtc cgcaggatat cggcgtgctg acgcgtaagc tgtatgccgc
     6241 gtttgaagca ttaccaggta aagtgacgct ggtaaacccg cagatttcac ccgatctctc
     6301 ggaaccgaat ctgaccttta tttatgtgcc gccgggccgt gctaaccgtt caggttggta
     6361 tctgtataac cgcgcgccaa atattgagtc gatcatcagc catcagccgc tggaatataa
     6421 ccgttacctg aacaaactgg tggcgtgggc atggtttaac ggcctgctga cctcgcgcac
     6481 ccgtttgtat attaaaggta acggcattgt cgatttgcct aagttgcagg agatggtcgc
     6541 cgacgtgtcg caccatttcc cgctgcgctt acctgcaccg acaccgaagg cgctctacag
     6601 cccgtgtgag atccgccatc tggcgattat cgttaacctg gaatatgacc cgacagcggc
     6661 gttccgcaat caggtggtgc attttgattt ccgtaagctg gatgtcttca gctttggcga
     6721 gaatcaaaat tgcctggtag gtagcgttga cctgctgtac cgcaactcgt ggaacgaagt
     6781 gcgtacgctg cacttcaacg gcgagcaatc gatgatcgaa gccctgaaaa ctattctcgg
     6841 caaaatgcat caggacgccg caccgccaga tagcgtggaa gtcttctgtt atagccagca
     6901 tctgcgcggg ttaatccgta ctcgcgtgca gcaactggtt tctgagtgta ttgaactgcg
     6961 cctttccagc acccgccagg aaaccgggcg tttcaaggcg ctgcgcgttt ctggtcaaac
     7021 ctggggcttg ttcttcgaac gcctgaatgt atcggtacag aagctggaga acgccatcga
     7081 gttttatggc gctatttcgc ataacaaact gcacggcctg tcagtgcagg ttgaaaccaa
     7141 tcacgtcaaa ttaccggcgg tggtggacgg ctttgctagt gaagggatca tccagttctt
     7201 cttcgaagaa acgcaagacg agaatggctt taatatctac attcttgacg aaagcaaccg
     7261 ggttgaggtg tatcaccact gcgaaggcag caaagaggaa ctggtgcgtg acgtcagtcg
     7321 cttctactcg tcatcgcacg accgcttcac ctacggctca agcttcatca acttcaacct
     7381 gccgcagttc tatcagattg tgaaggttga tggtcgtgaa caggtgattc cgttccgcac
     7441 aaaatctatc ggtaacatgc cgcctgccaa tcaggatcac gatacgccgc tattacagca
     7501 atatttttcg tgatgaacgt gccggaaagc gaggcttatc cggcatgcaa tcttagcgga
     7561 aactgactgt ttcacccgcc tgctgcgtcg ccgcctgttc cagcaaatcc cagaaggttt
     7621 cgccgctgcg atcgcaaatc cactcatcgc ctttcagatc aaaatggtag ccgccttgtt
     7681 tggttgccag ccatacctgg tgcagcggtt cctggcggtt gataatgatt ttgctgccgt
     7741 tttcaaaggt aatggtcagc acgccgccgt tgatttcgca gtcgatatcg ctgtcgccat
     7801 cccagtcgtc cagatgctct tcaatggtca gccacagttg atcagccagg cgatgaaatt
     7861 cactgtcgtt cattgttgta tcctgttttt aagtgatggc ggcagtatag cggcatgggg
     7921 tcagggcttc aaagtttgca cctctgctgc tgcgttccgg catgattcat ccgtcaccgg
     7981 aataatgatg tctctgtgtg gcgaaagatt tgtctcttca ttagggcgca gttacaccac
     8041 gcctttccct gtttctggta aacattatga tcaggtttac cgagcgagca tcctcacgct
     8101 gacggaacta aaaaagccaa caaacaaacc acattgcgat agtgcataaa gccatcctgg
     8161 cgcgaggtgc cgatcacgaa actgccagca gaacataaat cctcacgagt aagcgttata
     8221 ctcgcagcat ttcctcactt ttcagacttc ataaagagtc gctaaacgct tgcttttacg
     8281 ccttctcctg cgatgataga aagcagaaag cgatgaactt tacaggcaat ccataatgaa
     8341 aaacgtgttt aaggtactca ctgtattact tactctcttc agcctgacgg gctgcggtct
     8401 gaaaggtccg ctctatttcc cgcctgcaga taaaaatgca ccgccgccga ccaaaccggt
     8461 agagacgcaa acgcaatcca cggtgccgga taaaaacgat cgcgccactg ggatggtcca
     8521 tcccaggtga attactaaaa gtcagtttct gtacccgcgt gattggagta aatgatgcag
     8581 ttctcgaaaa tgcatggcct tggcaacgat tttatggtcg tcgacgcggt aacgcagaat
     8641 gtcttttttt caccggagct gattcgtcgg ctggcggatc ggcacctggg ggtagggttt
     8701 gaccagctgc tggtggttga gccgccgtat gatcctgaac tggattttca ctatcgcatt
     8761 ttcaatgctg atggcagtga agtggcgcag tgcggcaacg gtgcgcgctg ctttgcccgt
     8821 tttgtgcgcc tgaaagggct gaccaataag cgcgatatcc gcgtcagcac cgccaacggg
     8881 cggatggttc tgaccgtcac cgatgatgat ctggtccgcg taaatatggg cgaacccaac
     8941 ttcgaacctt ccgctgtgcc gtttcgcgct aacaaagcgg aaaagaccta tattatgcgc
     9001 gccgccgagc agacaatctt atgcggcgtg gtgtcgatgg gaaatccgca ttgcgtgatt
     9061 caggtcgatg atgtcgatac cgcggcggta gaaacgcttg gtcctgttct ggaaagccac
     9121 gagcgttttc cggagcgcgc caatatcggt tttatgcaag tggttaagcg cgagcatatt
     9181 cgtttacgcg tttatgagcg tggggcagga gaaacccagg cctgcggcag cggcgcgtgt
     9241 gcggcggttg cagtagggat tcagcaaggt ttgctggccg aagaagtacg cgtggaactc
     9301 cccggcggtc gtcttgatat cgcctggaaa ggtccgggtc acccgttata tatgactggc
     9361 ccggcggtac atgtctacga cggatttatt catctatgaa gcaaccaggg gaagaactgc
     9421 aggaaacact cacggagctt gatgaccggg cggttgtcga ttatctgatt aaaaatcctg
     9481 agttttttat ccgtaatgcg cgcgcagtag aagcgatacg tgtgccacat ccggtacgcg
     9541 gcaccgtttc gttggtcgag tggcacatgg cccgcgcacg taatcatatt catgttctgg
     9601 aagagaacat ggcgctgttg atggaacagg ctatcgccaa cgaaggcctg ttttatcgcc
     9661 tactctacct gcagcgcagt ctcaccgccg ccagcagtct cgacgatatg ctgatgcgct
     9721 ttcaccgctg ggcgcgcgat ctcggcctgg caggtgcgag tctgcgcctg tttccggatc
     9781 gctggcgctt aggtgcgccg tcgaaccaca ctcatctggc attaagccgt cagtctttcg
     9841 aaccgctgcg tattcagcgt ttggggcagg aacagcacta tcttgggccg cttaacggac
     9901 cagagctgct ggtggtgcta ccggaagcga aagcggtggg atcggtggcg atgtcgatgc
     9961 tgggaagcga tgctgatttg ggtgtcgtgc tgtttaccag tcgcgatgcc agtcactatc
    10021 aacaagggca aggaacgcag ttacttcatg aaattgcgct gatgttgccg gagcttctgg
    10081 agcgttggat tgaacgcgta tgaccgattt acacaccgat gtagaacgct acctacgtta
    10141 tctgagcgtg gagcgccagc ttagcccgat aaccctgctt aactaccagc gtcagcttga
    10201 ggcgatcatc aattttgcca gcgaaaacgg cctgcaaagc tggcagcaat gtgatgtgac
    10261 gatggtgcgc aattttgctg tacgcagtcg ccgtaaaggg ctgggagcag caagtctggc
    10321 gttacggctt tctgcgctac gtagcttttt tgactggctg gtcagccaga acgaactcaa
    10381 agctaacccg gcgaaaggtg tttcggcacc gaaagcgccg cgtcatctgc cgaaaaatat
    10441 cgacgtcgac gatatgaatc ggctgctgga tattgatatc aacgatcccc tcgctgtacg
    10501 cgaccgtgca atgctggaag tgatgtacgg cgctggtctg cgtctttccg agctggtggg
    10561 gctggatatc aaacacctcg acctggagtc tggtgaagtg tgggtaatgg ggaaaggcag
    10621 caaagagcgc cgcctgccga ttggtcgcaa cgctgtggcg tggattgagc actggcttga
    10681 tttgcgcgac ctgtttggta gcgaagacga cgcgcttttt ctgtcgaaac tgggcaagcg
    10741 tatctccgcg cgtaatgtgc agaaacgctt tgccgaatgg ggcataaaac aagggctgaa
    10801 taatcacgtt cacccacata aattacgtca ctcgttcgct acgcatatgc tggagtcgag
    10861 cggcgatctt cgtggtgtgc aggagctact gggtcatgcc aacctctcca ccacgcaaat
    10921 ctatactcat cttgattttc aacaccttgc ctcggtgtac gatgcggcgc atccacgcgc
    10981 caaacggggg aaataatgcg tttttaccgg cctttggggc gcatctcggc gctcaccttt
    11041 gacctggatg atacccttta cgataaccgt ccggtgattt tgcgcaccga gcgagaggcg
    11101 cttacctttg tgcaaaatta tcatccggcg ctgcgcagct tccagaatga agatctgcaa
    11161 cgcctgcgcc aggcggtacg ggaagcggaa cccgagattt atcacgacgt gacgcgctgg
    11221 cgttttcgtt cgattgaaca agcgatgctc gacgccgggc tgagtgccga agaagccagt
    11281 gcaggcgcac acgcagcaat gatcaacttt gccaaatggc gcagccgaat cgacgtcccg
    11341 cagcaaactc acgacacctt aaaacagctg gcgaagaaat ggccgctggt ggcgatcacc
    11401 aacggtaacg cccagccgga actgtttggt ttgggggatt attttgagtt tgtgctgcgc
    11461 gctggcccgc acgggcgctc aaaaccgttc agcgatatgt actttctggc tgcggaaaaa
    11521 ctcaacgtgc cgatcggcga gatcttacat gttggggacg atctcaccac tgacgtgggt
    11581 ggggcaattc gcagcggaat gcaggcctgt tggatcagac cggaaaatgg cgatctgatg
    11641 caaacctggg acagccgttt actgccgcat ctggaaattt cccggttggc atctctgacc
    11701 tcgctgatat aatcagcaaa tctgtatata tacccagctt tttggcggag ggcgttgcgc
    11761 ttctccgccc aacctatttt tacgcggcgg tgccaatgga cgtttcttac ctgctcgaca
    11821 gccttaatga caaacagcgc gaagcggtgg ccgcgccacg cagcaacctt ctggtgctgg
    11881 cgggcgcggg cagtggtaag acgcgcgtac tggtgcatcg tatcgcctgg ctgatgagcg
    11941 tggaaaactg cttgccatac tcgattatgg cggtgacgtt taccaacaaa gcggcggcgg
    12001 agatgcgtca tcgtatcggg cagctaatgg gcaccagcca gggcggcatg tgggtcggca
    12061 ccttccacgg gctggcgcac cgtctgctgc gtgcgcacca tatggacgcc aatctgccgc
    12121 aggattttca gatcctcgac agcgaggacc agctacgcct gctcaaacgc ctaatcaagg
    12181 cgatgaacct cgacgagaag cagtggccgc cgcgccaggc aatgtggtac atcaacagcc
    12241 agaaagatga aggcctgcgt ccacatcata ttcaaagcta cggtaatccg gtggagcaga
    12301 cctggcagaa ggtgtatcag gcgtatcagg aagcgtgtga ccgcgcgggc ctggtggact
    12361 tcgccgagct gctgctgcgc gctcacgagt tgtggcttaa caagccgcat atcctgcaac
    12421 actaccgcga acgttttacc aatatcctgg tggacgaatt ccaggatacc aacaacattc
    12481 agtacgcgtg gatccgcctg ctggcgggcg acaccggcaa agtgatgatc gtcggtgatg
    12541 acgaccagtc aatctacggc tggcgcgggg cgcaggtgga gaatattcag cgtttcctta
    12601 atgatttccc cggtgccgaa actattcgtc tggagcaaaa ctaccgctct accagcaata
    12661 ttctgagcgc cgctaacgcc ctgattgaaa acaataacgg gcgtctgggt aaaaaactgt
    12721 ggaccgatgg cgcggacggt gagcctattt ccctctattg cgcttttaac gaactcgatg
    12781 aagcgcgttt tgtggttaac cgcatcaaaa cctggcagga caacggcggg gcgcttgccg
    12841 agtgcgccat tctctaccgc agtaacgccc agtcgcgtgt gctggaagaa gcactgttgc
    12901 aggcgagtat gccgtaccgt atttacggcg ggatgcgctt cttcgaacgc caggaaatca
    12961 aagatgcgct ctcgtatctg cgcctgattg ccaaccgtaa cgacgacgcg gcctttgagc
    13021 gtgtggtgaa tacgccaacg cggggtattg gtgaccggac gctggacgtg gtacgtcaga
    13081 catcgcgcga tcgccagtta acactctggc aggcatgtcg tgaactgttg caggaaaaag
    13141 ccctcgccgg acgtgctgcc agcgccttac agcggtttat ggaactgatc gacgccttag
    13201 cgcaggaaac tgccgatatg ccgctgcatg tacagactga ccgggtaatt aaagactccg
    13261 gcctgcgtac catgtacgag caggagaagg gcgaaaaagg tcagacgcgt atcgaaaact
    13321 tagaggaact ggtgacggca acgcgccagt tcagctacaa cgaagaagac gaagatttaa
    13381 tgccgctgca ggcgttccta tcccatgcgg cgctggaagc aggcgaaggg caggcggata
    13441 cctggcagga tgcggtacaa ctgatgacca tgcactcggc gaaaggtctg gagttcccgc
    13501 aggtgtttat cgtcggtatg gaagagggca tgttcccaag ccagatgtcg ctggatgaag
    13561 gcgggcgtct ggaagaagaa cgccgtctgg cctacgttgg cgtaacccgc gcgatgcaga
    13621 aactgacgct gacctacgcg gaaactcgcc gtctgtatgg caaagaggtt taccatcgcc
    13681 cgtcgcgctt tattggcgag ctgccggaag agtgtgtgga agaggtgcgc ctgcgcgcca
    13741 cggtaagccg cccggtcagc catcagcgga tgggtacgcc gatggtcgag aacgacagcg
    13801 gctacaagct cggccagcgt gtacgccacg ctaagtttgg tgaaggcacc atcgtcaata
    13861 tggaaggcag cggtgaacat agccgtttgc aggtggcatt ccagggccag ggtattaaat
    13921 ggctggtggc ggcttatgcc cggttggaga cggtgtgacg ttgcgggatg cggtgcgcca
    13981 caccgcattc cgcatcagca ccttacttag cgcacttctt cacatcaata ttccagtcag
    14041 caccagacgc aacctgatgt ttctgcgcga aattatccat attcagcgcc acggaaacat
    14101 tcaataagct gttcagatac accatcggct ggccttccgg cacatcgccg aagcttgcga
    14161 catacggcat tttccctgcg tatttttgct gggaaccttc ggaaatggtc acacacaacg
    14221 tgtcgttgag tttgatttct gcctgattca gcaactcgtc gctgatattg ctccagacgt
    14281 tgccgtactg aatatcgaga atcgggatat tgcccttcac ttcacctttt gttgcttttg
    14341 ctttctgata agaaagttcg acaacttttg ctggaagctc tggccccacc tgttcgaacg
    14401 taatcgcccc ggaagccaga cgtgcaccgg tgtaggcata cacgtcacga ccgtggaagg
    14461 tatatgactt ttcagaacct ttcaggcggt tagctttttc gtcgatttcc cggacgctat
    14521 caatacccag agtttgtgcc accaaagtga gcgtaccgtt atctggtgaa acgaagtatt
    14581 gaccgctttt ggttttcagc actaccgatt tacggtcagt gcctacgccc ggatcgacca
    14641 cgctaacaaa caccgaacct tgcggccagt aactggcagt ctgatacagg cgataagcgc
    14701 cttcccagat gttatacggt ggaatttcgt gtgttaaatc aaagatttta agattatggt
    14761 caacgccgaa tgcgacccct ttcatcgccg agacggcacc atctttcagg ctaaaatcag
    14821 tttgtaaaat caaagcgtta tccgcatagg cggatgatga tcccagacac gttaataaaa
    14881 ataacgttgc gagcgttttt ttcatttttg tttcccttca tttatctgat gattttccag
    14941 ctgtgtagcc agtcccagtt tgtctggcct cctcgagtgt acaatgctta acctttccgc
    15001 tcgacggaaa tcatggtgac aaaaggatag cgttgccacg ggattgcccc gcctttcata
    15061 tacatatgtg aaattgtgcc atcaagataa agcaactgct caacgttcag tttcgctttg
    15121 gcataacagg caaaatcata aaaatttgtc gcctgctggc tcaacaaaaa cacggcgttc
    15181 ccttgtttat taatccctac accgttacga attttacgcg aggcaacatt cgggtgaatg
    15241 cgtggattaa ttacgccgtt ttctaataac attggccctg actgcaccgc aaactgaatc
    15301 tctttactgg ttttaaaggc atccagacga atgatgccga ctttatctcc cgcaacataa
    15361 aacacgccgc caggacggat aaagaaattc ccttcacctg aagcgagatt taacgccacc
    15421 ttctg
//
LOCUS       DAZPEB010000093        15270 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZPEB010000093 DAZPEB010000000
VERSION     DAZPEB010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15270
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.094"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            133..606
                     /gene="trmL"
                     /locus_tag="ACHURJ_002454"
     CDS             133..606
                     /gene="trmL"
                     /locus_tag="ACHURJ_002454"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFR1281360.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(659..1480)
                     /gene="cysE"
                     /locus_tag="ACHURJ_002455"
     CDS             complement(659..1480)
                     /gene="cysE"
                     /locus_tag="ACHURJ_002455"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFR1281361.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(1560..2579)
                     /gene="gpsA"
                     /locus_tag="ACHURJ_002456"
     CDS             complement(1560..2579)
                     /gene="gpsA"
                     /locus_tag="ACHURJ_002456"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFR1281362.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGAEPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(2579..3046)
                     /gene="secB"
                     /locus_tag="ACHURJ_002457"
     CDS             complement(2579..3046)
                     /gene="secB"
                     /locus_tag="ACHURJ_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFR1281363.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(3109..3360)
                     /gene="grxC"
                     /locus_tag="ACHURJ_002458"
     CDS             complement(3109..3360)
                     /gene="grxC"
                     /locus_tag="ACHURJ_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFR1281364.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(3502..3933)
                     /locus_tag="ACHURJ_002459"
     CDS             complement(3502..3933)
                     /locus_tag="ACHURJ_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFR1281365.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            4178..5722
                     /gene="gpmM"
                     /locus_tag="ACHURJ_002460"
                     /gene_synonym="pgmI"
     CDS             4178..5722
                     /gene="gpmM"
                     /locus_tag="ACHURJ_002460"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFR1281366.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            5756..7015
                     /gene="envC"
                     /locus_tag="ACHURJ_002461"
     CDS             5756..7015
                     /gene="envC"
                     /locus_tag="ACHURJ_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFR1281367.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQCGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            7019..7978
                     /locus_tag="ACHURJ_002462"
     CDS             7019..7978
                     /locus_tag="ACHURJ_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFR1281368.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPLTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(7965..8894)
                     /locus_tag="ACHURJ_002463"
     CDS             complement(7965..8894)
                     /locus_tag="ACHURJ_002463"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001340448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFR1281369.1"
                     /translation="MEIIIINDGSTDNSVEIAKHYAENYPHVRLLHQANAGASVARNR
                     GIEVATGKYVAFVDADDEVYPTMYETLMTMALEDDLDVAQCNADWSFRETGETWQSIP
                     SDRLRSTGVLTGPDWLRMGLSSRRWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWT
                     TEFMFNALRARYTEQSLYKYYLHNTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNY
                     ADKITIYPEFHQQITYEALRVCHAVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVK
                     VGYQALLWSFRLWQWRDKTRSHHRITRSAFNLR"
     gene            complement(9238..10263)
                     /gene="tdh"
                     /locus_tag="ACHURJ_002464"
     CDS             complement(9238..10263)
                     /gene="tdh"
                     /locus_tag="ACHURJ_002464"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFR1281370.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(10273..11469)
                     /gene="kbl"
                     /locus_tag="ACHURJ_002465"
     CDS             complement(10273..11469)
                     /gene="kbl"
                     /locus_tag="ACHURJ_002465"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFR1281371.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            11683..12615
                     /gene="rfaD"
                     /locus_tag="ACHURJ_002466"
     CDS             11683..12615
                     /gene="rfaD"
                     /locus_tag="ACHURJ_002466"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFR1281372.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            12625..13671
                     /gene="rfaF"
                     /locus_tag="ACHURJ_002467"
     CDS             12625..13671
                     /gene="rfaF"
                     /locus_tag="ACHURJ_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFR1281373.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTSQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNTLLLQEEA"
     gene            13675..14655
                     /gene="rfaC"
                     /locus_tag="ACHURJ_002468"
     CDS             13675..14655
                     /gene="rfaC"
                     /locus_tag="ACHURJ_002468"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFR1281374.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFDYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKRFIEENAENAAMI"
     gene            complement(14725..>15270)
                     /locus_tag="ACHURJ_002469"
     CDS             complement(14725..>15270)
                     /locus_tag="ACHURJ_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFR1281375.1"
                     /translation="YKLYCFIAIALLAGVFSFKDTLLMRMNDLNNDLVNYSHDNTRTS
                     VGARLAMYEVGLKTYSPIGQSLEKRAEKIHELEEKEPRLSGALPFVDSHLHNDLIDTL
                     STRGIPGVVLTILAFSAILIYALRTAKEPYILILLFSLLVVGLSDVILFSKPVPTAVF
                     VTIILLCAYFKAQSDQYLLDK"
BASE COUNT         3866 a         3984 c         3886 g         3534 t
ORIGIN      
        1 accctctccc tcagggagct gtctcttgat cagatctcct gatcaagaga cagggggagg
       61 gccggggtga ggggaaaagg ccgcacggtt caccaatttt ctgctatcct gcccccgcac
      121 taagggggca gtatgctaaa catcgtactt tacgaaccag aaattccgcc aaatactggc
      181 aacatcatcc gtctttgcgc taataccggc tttcgtctgc atatcatcga accgatggga
      241 tttgcctggg acgataagcg cctgcgccgc gcggggctgg actatcacga gtttaccgcc
      301 gttacgcgtc atcatgacta tcgcgcgttc ctcgaagcag aaaatcccca gcgcctgttt
      361 gccctcacca cgaaaggtac gcctgctcac agcgccgtaa gctatcagga tggcgattat
      421 ctgatgttcg gcccggaaac acgcggcctg ccagcgagca ttcttgatgc cctgcccgct
      481 gaacaaaaaa ttcgcattcc gatggtgccg gacagccgca gcatgaatct gtccaatgcg
      541 gtgtcggtag tggtgtatga agcctggcgg cagctgggat atccgggagc gttgttgaga
      601 gattagttac tgtatgccgg atagatgatt acatcgcatc cggcacgatc acaggacatt
      661 agatcccatc cccatactca aatgtatggt taataccgtt gaaatgctgg tccatatcca
      721 ttgatggctt atcgctgtct ggtttaccga caatacgagc cggaacgcca gcggcggttg
      781 tatgcggcgg caccggttgc agcaccacgg aacctgcgcc aatcttcgcg ccgcgaccaa
      841 cttcaatatt gccgaggatt ttcgcgcccg cgccaatcat cacaccttca cgaattttcg
      901 ggtgacggtc accaccagat ttacccgtac cgccaagcgt cacagattgc agaatcgata
      961 cgtcgttttc aattaccgcc gtttcaccca caacgatgcc cgtggcgtgg tcgagcataa
     1021 tgccacggcc aatttttgct gccgggtgaa tatcgacctg gaacgtcaca gaaacctggt
     1081 tttgcagaaa gattgccaat gcgcgacgcc cctgattcca caaccagtga ccgatgcgat
     1141 aggcctgcaa ggcatgaaaa cccttcaggt ataacaacgg ggttgagtat ttatcgactg
     1201 ccgggtcgcg ggtacgcacc gcctgaatat cacaggccgc agaggcgatc atttccgggt
     1261 cagcggcgta ggcttcttcc accacttcac ggatagcaat agcaggcata attggcgatg
     1321 acagcttgtt cgccagcatg tagctcagtg cactaccaag gttttcgtgc ttgagtagcg
     1381 tcgcgtggta aaaactggcc agcattggct cacagtccgc cagcgttctg gcttcggctt
     1441 taatattgtt ccagacaatt tccagttctt cacacgacat tacttactcc acacgatgag
     1501 ataatgaccg gcccgttctg cgcgggccgg gtcatagcgg taacaaaggt tccctggggt
     1561 tagtggctgc tgcgctcgtc cttgcgtgca cgacctaata acgtcaatgc tgcctcgcgc
     1621 gcgttttttc cgcaatataa tacttgataa atttcctcgg ttattggcat ttctacgccg
     1681 aagcgatgcg ccagttcgcg gacttctttc gtattgcggt agccttccac cacctgacca
     1741 atcttctcct gcgcgctttg tacatccatg ccctgaccga gcatcatgcc aaaacggcgg
     1801 ttacgcgact ggttgtcggt acaggtaagc acaagatcgc ccagccccgc catgcccata
     1861 aaggtggcag gctcggcacc cagcgccgcg ccaagacgcg acatttcagc cagcccacgg
     1921 gtgatcagtg ccgtacgcgc attcgcacca aaaccgatac cgtcggacat ccccgcgcca
     1981 atggcaatga cgtttttcac cgcaccgcca agctgcacac cgatgaaatc cgggttgctg
     2041 taaacgcgga aacttttgcc acagtgcagc aattgttgga gatcatcggc aaaggtctgg
     2101 tcggtagagg ccaacgaaat agccgtcggt aaacctgccg ccagttcttt cgcaaacgtt
     2161 gggccagaga taaccgccag cggaatttga tcgcctaagg cctcacgcgc cacgtcctgt
     2221 aacagacgtc cggtttccgc ttccagccct ttggtcgccc acaccagacg cgcatcagga
     2281 cgcatcagcg gtttaatctg gcgcagcact tcaccaaaga catggctggg tacgacgacg
     2341 agaatattac ggctggctgc cagcgcagtg gcgagatcgc tttcaagatg gagcgtatcg
     2401 ggaaaaggca catcggggag aaacgcggcg ttacagcggt cgcgttcaag cgttgcgata
     2461 tgttcagggt catggcccca gaggacaacc tcgtggccat ttcttgccag ggtaatggca
     2521 agagcggtgc cgtacgagcc ggcaccgatc acagtcattg aagcattacg ttggttcatc
     2581 aggcatcctg atgttcttca gtaccttcgc cagcctgctg ctgcaaatag ttcatgaaca
     2641 gcgcatcgaa gttaaccggc gcaaggttca gttgcgggaa tgtaccgcgg gataccatgc
     2701 tggtgatgca ctcacgagca tacgggaaca gaatgttcgg gcagtatgcg cccaggcaat
     2761 gtgccatctg ggtgccttcg atacccgcga tggagaaaat accgccctgc tgaacttcac
     2821 acaggaacgc ggtttcttcg cccagggagg ccgttacggt aacacgcagt accacttcgt
     2881 atacgtcatc tgccagttgg gaagatgccg tatccagatc aagtttaact tctggttgcc
     2941 aatctttctg gaaaacgtgc ggcgcgttcg gcgcttcgaa agagatatcc ttggtataaa
     3001 tacgttggat ctggaaagtc atttcagtgt tgttttgttc tgacatgtgt agaaaaccct
     3061 taagtgttgt ccttaaatac tgcgcaatgt aatgccaacg ttcacacgtt atttcagcag
     3121 gggatccagt ccaccacgtg catccaatgc atacaagtca tcacagccgc caatgtgctg
     3181 tgcgtcaata aaaatctggg gaaccgtggt gcgaccgctg cgtttgatca tctcttcacg
     3241 cttggcggca ttgccatcga tcggcaactc ctgaaaactc acgcccttgc tgctcagcag
     3301 tgcttttgca cgatggcaat acgggcaggt ttctttggta tagatttcaa cattggccat
     3361 aatttagctc ctgttttttt accctgaaat ttcatgttgc agggcggctg caaaatggag
     3421 tgtccccaga agcttataac aataagtcgc tgggggccta attgcagcta acggcctgca
     3481 tcatgaaaga cgacaggtaa attatttgcc gcgcaccaga ggcaagtttt cgccagccca
     3541 gccagcaacg ccctctttca gcacgaatac ttgcgcaaaa ccagctttcg tcagcgcgtt
     3601 tgcaggctcc tggcactgca tgccagaacc gtctaccacg ataaccggtt tgtctttgtg
     3661 cttctcaagc tcaccaacat tgttggcttt gatttcgctc ggcaacagat tgatagaacc
     3721 tgcgatatgg cctttacgga agtcatcacg ctgacgtaaa tccacaacca cagcgtcttc
     3781 tttgttgatc agacgcgtag cttcaccacg agtaatcacc ttcactttcg aggtcaggct
     3841 cttaaacgta gtcacaagaa ccgccaccag taacgcgatc caagcgatac tcagtatggg
     3901 atgacggcca acaaattgca taatttcttg catggggggt aacaactccc gacgtagtga
     3961 ttaaaaaaac caggaaagga gtatacctgc gcggtgcggc aaatacagcc agcgcgttaa
     4021 ctggaatgca attttgcggg gcacgacgaa aaaaaagcgc aaatctggca tcggcttgcc
     4081 gcgagcggtc gtatattttg atcttcagag gctattttat cgattcagct gtagtaaaat
     4141 tacgcaaatt ttgactcttg agtatgaggt tgtcgcaatg tcggtttcta aaaaacctat
     4201 ggtactggtg attctggatg gctatggcta tcgtgaagaa cagcaggata acgccatttt
     4261 tagtgctaaa accccggtaa tggatgcact gtgggccaat cgtccgcata ccctgatcga
     4321 cgcttccggt ctggaagtcg gtctgcctga ccgtcagatg ggtaactccg aagtaggcca
     4381 cgttaacctg ggtgccggcc gcatcgtgta tcaggacctg actcgtctgg acgttgaaat
     4441 caaagatcgc gctttctttg ctaatccagt gctgactggc gcggtagata aagcgaaaaa
     4501 cgcaggtaaa gcagtacaca ttatgggtct gctctccgca ggcggcgtac acagccacga
     4561 agatcacatc atggcgatgg tagaactggc agctgaacgc ggcgcagaaa aaatctacct
     4621 gcacgcattc cttgacggtc gcgacacccc gccgcgcagt gctgaatcct cgctgaaaaa
     4681 atttgaagag aaatttgctg cgctgggtaa aggccgcgta gcgtccatca ttggtcgtta
     4741 ctacgcgatg gaccgcgata accgttggga tcgcgttgaa aaagcctatg acctgctgac
     4801 tctggcgcag ggtgagttcc aggccgatac tgccgttgct ggtttgcagg cagcttatgc
     4861 tcgcgacgaa aacgacgagt tcgtgaaagc gaccgttatt cgtgcagaag gtcagccaga
     4921 tgcggcaatg gaagacggcg atgcgctgat tttcatgaac ttccgtgctg accgcgcgcg
     4981 cgaaatcact cgtgcattcg taaacgcaga tttcgacggc ttcgcgcgta agaaagtggt
     5041 taacgtcgat ttcgtgatgc tgaccgaata cgccgctgac atcaaaactg cggttgctta
     5101 cccacccgct tccctggtta acaccttcgg cgagtggatg gcgaaaaacg acaaaactca
     5161 gttgcgtatt tccgaaaccg aaaaatatgc ccacgttact ttcttcttca acggtggcgt
     5221 agaagagtca ttcaaaggcg aagatcgcat tctgatcaac tcacctaaag tggctaccta
     5281 cgatctgcaa ccggaaatga gctccgcaga gctgaccgaa aaactggttg cggccatcaa
     5341 gagcggcaaa tacgacacca ttatctgtaa ctatccgaac ggcgacatgg taggtcacac
     5401 cggggtgatg gaagcggcgg ttaaagcggt tgaagcactg gatcactgcg tggaagaagt
     5461 cgcgaaagcg gttgagtccg tgggtggaca actgctgatc accgctgacc acggtaacgc
     5521 tgaacagatg cgcgatccgg caacgggtca ggcacacacg gcacacacca acctgccagt
     5581 tccgctgatt tacgttggtg ataagaacgt gaaagcggtt gcaggcggca aactttctga
     5641 catcgcgccg accatgttgt cgctgatggg tatggaaatc ccgcaagaga tgactggtaa
     5701 gccgctgttc atcgtggaat aatccctccc catgagggga aaggcgatta ataccatgac
     5761 acgggccgtg aaaccgcgca ggtttgcaat caggcccatc atctacgcca gcgttcttag
     5821 cgctggcgta ttgttgtgcg ccttttccgc ccacgcggat gagcgtgacc aactcaaatc
     5881 tattcaggcc gatatcgccg caaaagagcg cgcggtacgc caaaagcaac aacaacgcgc
     5941 aagcctgctc gcacaattga aaaagcagga agaagcgatc tctgaagcca cccgtaagct
     6001 gcgcgaaacg caaaacacgc tcaatcaact caataaacag attgatgaga tgaacgcgtc
     6061 gattgccaaa ctggagcagc aaaaagccgc ccaggagcgc agcctcgccg cacaactgga
     6121 tgccgcattc cgtcagggcg agcataccgg tattcagcta attctcagcg gtgaagaaag
     6181 ccagtgtgga cagcgtttac aggcttattt cggctatctc aaccaggcgc gacaagaaac
     6241 cattgcccag ttgaagcaaa cgcgtgaaga agtcgccatg cagcgtgctg aactggaaga
     6301 gaaacaaagc gagcaacaaa cgctgttata tgaacagcgc gcccaacagg cgaaactgac
     6361 tcaggctctg aacgagcgta aaaagacgct ggcggggctg gaatcttcca tccagcaagg
     6421 tcagcagcag ctaagtgaac tgcgtgccaa cgaatcccgt ctgcgtaaca gcattgcccg
     6481 tgcggaagcc gcggcgaaag cgcgtgctga acgagaagcg cgtgaggccc aggcggttcg
     6541 cgaccgccag aaagaagcga cgcgcaaagg caccacctac aaaccgaccg aaagcgaaaa
     6601 atcgctgatg tcccgaactg gtggtctggg tgcgccgcgt ggtcaggcat tctggccggt
     6661 tcgcgggccg acgctgcatc gctatggcga gcagctacag ggtgaattac gctggaaagg
     6721 gatggttatc ggtgcctctg aaggtactga agttaaagca attgccgacg gtcgagtgat
     6781 tctggctgac tggctgcaag gttacggtct ggtggtggtg gttgagcacg gtaaaggcga
     6841 catgagtctt tacggctata atcagagcgc actggtgagc gttggttcgc aggttcgcgc
     6901 gggccagcca attgcactgg tgggcagcag tggcggtcag ggtcggcctt cactctattt
     6961 cgaaattcgc cgccagggtc aggcggtcaa tccacagccg tggttgggaa gataagtttt
     7021 gtttccattt cgtcgtaacg ttcttgcatt tgccgctctg ttggcgctct cctcccccgt
     7081 acttgctggc aaacttgcca tcgtcattga tgattttggg tatcgcccgc acaacgaaaa
     7141 ccaggtgctg gcgatgccct ccgctatctc cgtcgctgta ttacccgatt caccgcacgc
     7201 cagagagatg gcgaccaaag cgcataacag cgggcacgaa gtgttgattc atctcccgat
     7261 ggcaccactg agtaaacagc cgctggagaa aaatacgctg cgcccggaga tgagcagcga
     7321 cgaaattgag cgcattattc gtagtgcggt caataacgtg ccctatgccg tggggatcaa
     7381 caaccacatg ggtagcaaga tgacctctaa cctgtttggt atgcagaaag tgatgcaggc
     7441 actggagcgt tacaatcttt acttcctcga cagcgtaacc atcggtaata cccaggcgat
     7501 gcgcgccgcg caaggtactg gcgtgaaggt tattaaacgg aaggtgttcc tcgacgattc
     7561 gcagaacgaa gcggacatcc gtgtgcaatt taatcgcgca attgatctgg cgcgtcgcaa
     7621 cggttcaacc attgccattg ggcatcctca ccctttaacg gtacgcgtgc tacaacagat
     7681 ggtttataac ctgccgccag acattacgct ggtgaaagcc agcagcttgc tgaatgaacc
     7741 gcaggttgat acttctacac cgccgaaaaa cgctgtgcct gatgcaccgc gtaatccgtt
     7801 ccgtggcgtg aagctgtgca aaccgaagaa accgatagaa cctgtttatg ctaatcgctt
     7861 ctttgaagta ttaagcgaaa gcatcagcca gagcacgctg atcgtttact tccagcatca
     7921 gtggcaaggc tggggcaaac agcccgaagc ggcgaagttt aacgctagcg caaattaaag
     7981 gcgctacgcg taatgcgatg gtgcgaccgc gttttgtcgc gccattgcca taagcggaaa
     8041 gaccacagta acgcctggta accgaccttc acgctgcgca cattggtaat caggcgctta
     8101 tacataccgg aagtaaatat ctcggcaatc atccgttggc gggtaagaat atccggctct
     8161 ttgcgcaccg catggcaaac acgcaatgct tcgtaagtta tttgctgatg aaattccgga
     8221 taaatcgtaa ttttgtcggc ataatttcga tttaatttct ccagcaggcg ggtaatctta
     8281 atatagtgac gttgataatt aaggttttta ttcccttgtc tatgcaaccg actcactgac
     8341 gtattatgca gataatattt atataatgat tgctcggtat atcgcgcacg cagcgcgtta
     8401 aacatgaatt ctgttgtcca gacaatatcc tgatgatgta atccggcaat aaatttaatg
     8461 ttatttttaa caataacatc acggcgataa acccccatcc agacaacgtg agtccaacgg
     8521 cgcgaagaaa gccccatccg cagccagtcc gggccggtta atacgccggt tgagcgaagg
     8581 cgatcgctgg ggatggattg ccaggtttct cccgtttcac gaaaagacca gtcagcgttg
     8641 cactgcgcca cgtcgaggtc gtcctctaac gccatggtca tcagcgtttc gtacatggtg
     8701 ggatagacct catcgtcagc atcgacaaaa gcgacatatt tgcccgtcgc cacctcgatc
     8761 ccacgattac gcgctaccga tgcgccagca ttcgcctgat gcaacaaacg aacgtgcgga
     8821 tagttttctg cgtaatgctt tgctatttca acagaattat ccgttgaacc atcgttaata
     8881 ataatgattt ccagagcagt ctaggtttgc gtaattaaag attccataca agtgcggaaa
     8941 tcatcgcccg cattatataa cggaataata acactaagtt tattggtgct gttcatcata
     9001 aattaccgac gacctctgaa gttatcctga cgttttacag gctttgtatt aagaaactat
     9061 taagcctgtg agatattatt tttacaggtg agagagtcac ttcagggtag agagataatg
     9121 agaacagcta tataagagat cagcgcaatt acgaactctt tattacactg acaggatttt
     9181 acatattcaa cagattatcc ccagataaaa gggctggaat accagccctt gttcgtgtta
     9241 atcccagctc agaattactt tcccggactg gcccgaacgc atagcgtcaa agcccttctg
     9301 gaaatcatcg atagagaaac gatgggtaat gatcggcgag agatcgaggc cagactgaat
     9361 cagcgccgcc atcttgtacc aggtttcaaa catctcacga ccgtaaatac ctttaatgaa
     9421 caagccttta aagatcactt tggtccagtc gatagacata tcagacggcg gaatacccag
     9481 catcgcaata cggccgccgt ggttcatggt gtcaagcatg gtacgaaacg ctggcggcgc
     9541 accggacatt tccagaccga catcaaagcc ttcggtcatg cccagttcag ccatcacatc
     9601 attaaggttt tctttcgcga cgttaaccgc acgggtgata cccattttgc gcgccagctc
     9661 aaggcggtat tcgttaacat cagtgatcac cacattgcgt gcaccaacgt gtttcgccac
     9721 cgccgcagcc ataataccta tcggacctgc accagagacc agcacatcct cgccaaccag
     9781 atcgaacgac agcgcggtat gcacggcgtt accgaaggga tcaaaaatgg aagccaggtc
     9841 gtcggagata ttgtctggga ttttgaaggc gttgaacgcc gggatcacca gatattcggc
     9901 gaagcagccc ggacggttaa cgccgacgcc gatcgtgttg cggcacagat gggtacgacc
     9961 gccacgacag ttgcgacagt gaccgcaggt gatatggcct tcgccagaaa cgcgatcgcc
    10021 gattttgaag cctttcactt cctgaccaat acctaccact tcaccgacat attcatggcc
    10081 tacgaccatc ggcaccggga tggttttttg cgaccactca tcccagttat agatgtgaac
    10141 gtcagtcccg cagatggctg ttttacggat tttaatcagc agatcgttat gcccgagttc
    10201 cggtacaggg acgtcggtca tccagatgcc ctcttccgct ttcagtttgg ataacgcttt
    10261 catctcacat cctcaggcga taacgcccag ttgtttacca atacgcgtga atgcttctac
    10321 tgcacgcgta atttgctcag gggtatgcgc cgcagacatc tgggtacgaa tacgcgcctg
    10381 acctttcgga acgaccggat agaagaaacc ggtgacgtaa atgccctctt tttgcagctc
    10441 tcgggcaaat ttctgcgcca ctaccgcatc accaagcatg accggaataa tggcgtgatc
    10501 ggctcccgcc agggtaaagc ccgccgccga catttgctca cggaactgac gcgcgttcgc
    10561 ccacagacgg tcacgcagtt cgctgcccgc ttcgaccatc tccagtactt tgatggacgc
    10621 ggcaacaatg gccggtgcca gcgagttgga gaacaggtac ggacgagaac gctggcgcag
    10681 ccactcaacc acctctttgc gcgccgcggt ataaccacca gaagccccgc ccagcgcttt
    10741 accaagcgta ccggtgataa tatcgacccg gcccatcaca tcgcagtatt catgggaacc
    10801 acgaccattt tcaccgacaa aaccgaccgc gtgggagtca tccaccatca ccaaggcatc
    10861 gtatttatct gccagatcgc aaacgccttt caggttggca atcacgccgt ccattgagaa
    10921 cacaccatcg gtggcaatca gcacatgacg cgcaccggct tcacgcgctt ctttcagacg
    10981 tgcttccagc tcctgcatat cgttgttggc atagcgatag cgtttagctt tgcacagacg
    11041 cacaccatca ataatagacg cgtggttcag tgcgtcggag ataatggcgt cttccgcacc
    11101 cagcagcgtt tcaaacagtc caccgttagc atcaaagcag gaagagtaga gaatcgcatc
    11161 ttccatcccc aggaatgccg ccagtttttg ttcaagctct ttatggctgt cctgagtgcc
    11221 gcaaataaaa cgcaccgaag ccatgccgaa accgtgagaa tccattcccg cctttgctgc
    11281 cgcaatcaga tccggatgat tcgccagccc gagatagttg ttggcacaaa agttaatgac
    11341 gtggcttcca tcagccacag tgatatctgc ttgctgcgca gacgtaataa tgcgctcttc
    11401 tttaaacaac ccttccgccc gtgcggtttc cagatcgttg gttaactgct gataaaattc
    11461 tccacgcatt gcgattctcc agacagggca aattccagca catattaccc aaacttatac
    11521 gtcgcgacga gatgacgcgt taacacttct gcaaaattca ggataaataa cgtccatcaa
    11581 tctcactttt ggcgaatggc tgaagccaac gccgtatagt gatatgatag aagtattcgt
    11641 gtctgagatt gtctctgact ccataattcg aaggttacag ttatgatcat cgttaccggc
    11701 ggcgcgggct ttatcggcag caacatcgtt aaggccctga atgataaagg catcaccgat
    11761 attctggtgg tggacaacct gaaagacggc accaagtttg tgaacctggt ggatctggat
    11821 atcgcggact atatggataa ggaagacttc ctgatccaga ttatggctgg cgaagagttc
    11881 ggcgatgtcg aagcgatttt ccacgaaggc gcatgctctt ccaccaccga gtgggacggc
    11941 aagtatatga tggataacaa ctatcaatac tccaaggagc tgctgcacta ctgcctggag
    12001 cgcgaaatcc cgttcctgta cgcttcttcc gcagccacct acggcggacg cacctccgac
    12061 tttattgaat cccgcgagta cgaaaaaccg ttgaacgtct acggttactc aaaattcctg
    12121 tttgatgaat atgttcgcca aatcctgcct gaagcgaact cgcagattgt tggcttccgc
    12181 tatttcaacg tttatggacc gcgtgaaggc cataaaggca gcatggcgag cgtcgctttc
    12241 catctcaaca ctcagcttaa caacggtgaa tcaccgaagc tgtttgaagg tagcgagaac
    12301 ttcaaacgtg acttcgtcta tgtgggcgac gtggcagatg taaacctgtg gttcctggaa
    12361 aatggcgttt ccggtatctt caacctcggt actggtcgtg cggaatcctt ccaggccgta
    12421 gcagatgcta ctctggctta tcacaagaaa ggccagatcg aatacattcc gttcccggat
    12481 aagctgaaag gccgctacca ggcgttcact caggcagatc tgacaaatct gcgcgcggcg
    12541 ggttacgaca aaccgttcaa aaccgttgct gaaggtgtaa cggaatacat ggcctggctg
    12601 aatcgcgacg cataagagct ctgcatgaaa atactggtga tcggcccgtc ttgggttggc
    12661 gacatgatga tgtcgcaaag tctctatcgc acgctccagg cgcgctatcc ccaggcgata
    12721 atcgatgtga tggcaccggc atggtgccgt ccattattat cgcggatgcc ggaagttaac
    12781 gaagctatcc ctatgcctct cggtcacgga gcgctggaaa tcggcgaacg ccgcaaactg
    12841 ggtcatagcc tgcgtgaaaa gcgctacgac cgcgcctacg tcttacccaa ctccttcaaa
    12901 tctgcattag tgcctttctt cgcgggtatt cctcatcgca ccggctggcg cggcgagatg
    12961 cgctacggtt tactcaacga tgtacgcgtg ctcgataaag aagcctggcc gctaatggtg
    13021 gaacgctatg tcgcgctggc ctatgacaaa ggcattatgc gtacctcaca agatctgccg
    13081 cagccattgt tatggccgca gttgcaggtg agcgaaggtg aaaaatcata tacctgtaat
    13141 caattttcgc tttcatcaga acgtccgatg attggctttt gcccgggtgc ggagtttggt
    13201 ccggcaaaac gctggccaca ctaccactat gcggaactgg caaagcagct gattgatgaa
    13261 ggttatcagg tggttctgtt tggctctgcg aaagatcatg aagcgggcaa tgagattctt
    13321 gccgctttga ataccgagca gcaggcatgg tgtcggaacc tggcggggga aacacagctt
    13381 gatcaagcgg ttatcctgat tgcagcctgt aaagccattg tcactaacga ttctggcctg
    13441 atgcacgttg cggcggcgct caatcgtccg ctggttgccc tgtatggtcc aagtagcccg
    13501 gacttcacac cgccgctatc ccataaagcg cgcgtgatcc gtctgattac cggctatcac
    13561 aaagtgcgta aaggtgacgc tgcggagggt tatcaccaga gcttgatcga cattactccc
    13621 cagcgcgtac tggaagaact caacacgcta ttgttacaag aggaagcctg acggatgcgg
    13681 gttttgatcg ttaaaacatc gtcgatgggc gatgttctcc atacgttgcc cgcactcact
    13741 gatgcccagc aggcaatccc agggattaag tttgactggg tggtggaaga agggttcgca
    13801 cagattcctt cctggcacgc tgccgttgag cgagttattc ctgtggcaat acgtcgctgg
    13861 cgtaaagcct ggttctcggc ccccataaaa gctgaacgca aagcgtttcg tgaagcgcta
    13921 caagcagaga actatgacgc agttatcgac gctcaggggc tggtaaaaag cgcggcactg
    13981 gtgacacgtc tggcgcatgg cgtaaagcat ggattggact ggcaaaccgc tcgcgaacct
    14041 ttagccagcc tgttttacaa ttgtaagcat catattgcaa aacagcagca cgccgtagaa
    14101 cgcacccgcg aactgtttgc caaaagtttg ggctatagca aaccgcaaac ccagggcgat
    14161 tatgctatcg cacagcattt tctgacgaac ctgcctacag atgctggcga atatgccgta
    14221 tttcttcatg cgacgacccg tgatgataaa cactggccgg aagaacactg gcgagaattg
    14281 attggtttac tggctgattc aggaatacgg attaaacttc cgtggggcgc gccgcatgag
    14341 gaagaacggg cgaaacgact ggcggaagga tttgattatg ttgaagtatt gccgaagatg
    14401 agtctggaag gcgttgcccg cgtgctggcc ggggctaaat ttgtagtgtc ggtggatacg
    14461 gggttaagcc atttaacggc ggcactggat agacccaata tcacggttta tggaccaacc
    14521 gatccgggat taattggtgg gtatgggaag aatcaaatgg tttgtagggc tccggggaat
    14581 gagttgtctc aattgacagc aaatgctgtt aagcgattca ttgaagaaaa cgctgaaaac
    14641 gctgctatga tttaaattac aaccccttaa tagataattt tgaaataaaa tcagcctcct
    14701 tgctgatttt atttcatata ttcattactt atctaataaa tattggtctg attgtgcttt
    14761 aaaataagca caaagcaata ttatggtgac aaacacagca gtcggaaccg gtttagaaaa
    14821 gagtattaca tcacttaggc ctactaccag tagtgaaaaa agcaagatta aaatataagg
    14881 ttctttagca gttcttaagg catatatgag tattgctgaa aatgctaaaa ttgttaatac
    14941 aactccagga ataccacgcg ttgataacgt atctatgaga tcgttatgca aatgagaatc
    15001 tacaaaaggt aaagcgccac tcaatctagg ctctttttct tctagctcat gtattttttc
    15061 tgcacgtttt tccagtgatt gtcctattgg agaatatgtt tttaagccaa cttcatacat
    15121 tgccagacgg gcaccgactg aagttctggt gttatcatgc gaataattaa ctaaatcgtt
    15181 atttaagtca ttcattctca taagcaatgt atctttaaaa gaaaatacac ctgctaataa
    15241 tgcaatcgca ataaaacaat ataacttata
//
LOCUS       DAZPEB010000094        15141 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZPEB010000094 DAZPEB010000000
VERSION     DAZPEB010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15141
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.095"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..421
                     /locus_tag="ACHURJ_002470"
     CDS             <1..421
                     /locus_tag="ACHURJ_002470"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFR1281376.1"
                     /translation="LMNNSDARKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRL
                     AEFGGLRHKAYRLGGDEFAMVLYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTL
                     SIGYAMTIEHASAEKLQELADHNMYQAKHQRAEKLVR"
     gene            437..919
                     /locus_tag="ACHURJ_002471"
     CDS             437..919
                     /locus_tag="ACHURJ_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFR1281377.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(996..1343)
                     /gene="rplS"
                     /locus_tag="ACHURJ_002472"
     CDS             complement(996..1343)
                     /gene="rplS"
                     /locus_tag="ACHURJ_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFR1281378.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(1385..2152)
                     /gene="trmD"
                     /locus_tag="ACHURJ_002473"
     CDS             complement(1385..2152)
                     /gene="trmD"
                     /locus_tag="ACHURJ_002473"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFR1281379.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(2183..2731)
                     /gene="rimM"
                     /locus_tag="ACHURJ_002474"
     CDS             complement(2183..2731)
                     /gene="rimM"
                     /locus_tag="ACHURJ_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFR1281380.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(2750..2998)
                     /gene="rpsP"
                     /locus_tag="ACHURJ_002475"
     CDS             complement(2750..2998)
                     /gene="rpsP"
                     /locus_tag="ACHURJ_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFR1281381.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(3135..4496)
                     /gene="ffh"
                     /locus_tag="ACHURJ_002476"
     CDS             complement(3135..4496)
                     /gene="ffh"
                     /locus_tag="ACHURJ_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFR1281382.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            4663..5454
                     /locus_tag="ACHURJ_002477"
     CDS             4663..5454
                     /locus_tag="ACHURJ_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFR1281383.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            5475..6761
                     /locus_tag="ACHURJ_002478"
     CDS             5475..6761
                     /locus_tag="ACHURJ_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFR1281384.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(6816..7409)
                     /gene="grpE"
                     /locus_tag="ACHURJ_002479"
     CDS             complement(6816..7409)
                     /gene="grpE"
                     /locus_tag="ACHURJ_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFR1281385.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            7532..8410
                     /gene="nadK"
                     /locus_tag="ACHURJ_002480"
     CDS             7532..8410
                     /gene="nadK"
                     /locus_tag="ACHURJ_002480"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFR1281386.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCKKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            8496..10157
                     /gene="recN"
                     /locus_tag="ACHURJ_002481"
     CDS             8496..10157
                     /gene="recN"
                     /locus_tag="ACHURJ_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFR1281387.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMAYGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALATQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            10306..10647
                     /gene="bamE"
                     /locus_tag="ACHURJ_002482"
     CDS             10306..10647
                     /gene="bamE"
                     /locus_tag="ACHURJ_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFR1281388.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(10709..10999)
                     /locus_tag="ACHURJ_002483"
     CDS             complement(10709..10999)
                     /locus_tag="ACHURJ_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFR1281389.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(10989..11426)
                     /gene="ratA"
                     /locus_tag="ACHURJ_002484"
     CDS             complement(10989..11426)
                     /gene="ratA"
                     /locus_tag="ACHURJ_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFR1281390.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            11597..12079
                     /gene="smpB"
                     /locus_tag="ACHURJ_002485"
     CDS             11597..12079
                     /gene="smpB"
                     /locus_tag="ACHURJ_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFR1281391.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            12294..12656
                     /gene="ssrA"
                     /locus_tag="ACHURJ_002486"
     tmRNA           12294..12656
                     /gene="ssrA"
                     /locus_tag="ACHURJ_002486"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(12817..15141)
                     /locus_tag="ACHURJ_002487"
                     /pseudo
     CDS             complement(12817..15141)
                     /locus_tag="ACHURJ_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
BASE COUNT         3710 a         4064 c         3549 g         3818 t
ORIGIN      
        1 gttgatgaac aattccgatg cccgaaaaac gtcggcgtta ctatttcttg atggcgataa
       61 tttcaaatat atcaatgata cctggggtca tgcgacgggc gatagggtct tgattgaaat
      121 cgcaaaacgg ttagctgaat ttggcgggct gcgacataaa gcataccgcc tgggcggcga
      181 tgaattcgct atggtgctct atgatgtaca gtcggaatct gaagtgcaac agatatgctc
      241 agcactgaca caaatcttta atctcccgtt tgatcttcat aatggccatc agaccaccat
      301 gacattaagc attggttacg cgatgaccat tgagcacgcc tctgcggaaa aattacaaga
      361 gcttgccgat cacaatatgt atcaggccaa acaccagcgt gccgaaaagc tggtgagata
      421 acaaggatat atcgatatga taaagcacct ggtagccccc ctgattttca cctcactaat
      481 actgactggc tgccagtccc ctcagggaaa gtttactcct gagcaagtcg ccgctatgca
      541 atcttatgga tttactgaat ccgccggcga ctggtcgctg ggcttatcag atgccattct
      601 gttcgcaaaa aatgactaca aattgctccc ggaaagccag caacagatcc aaaccatggc
      661 agccaaattg gcctcgacag ggctaacaca tgcccgtatg gatggacaca ccgataacta
      721 tggtgaagac agttacaacg aaggcttatc attgaaacgg gcgaatgtcg tggccgatgc
      781 atgggctatt ggtggacaaa ttccacgcag caatctcacc acacagggtt taggaaaaaa
      841 atatcccata gccagtaaca agaccgccca gggccgcgcc gagaaccgcc gcgtcgcagt
      901 ggtgattaca accccttaaa acacgagcaa acagacaaaa aaagggccag ccaattggcc
      961 agcccttctt aacaggatgt cgcttaagcg aaatcttagt taagacgctc tttgatacga
     1021 gcagccttac cagtacgctc acgcaggtag tacagtttag ctttacgaac agcaccacga
     1081 cgtttgacag aaatgctgtc aactaccgga gagtgagtct ggaagacacg ctcaacgcct
     1141 tcgccgttgg aaattttacg aacagtgaat gcagagtgca gaccgcggtt acgaatagcg
     1201 ataaccacgc cctcgaatgc ctgcagacgt tttttggaac cttcaacaac ccatactttc
     1261 acttccacgg tatcacccgg acggaaggaa ggtacgtcct gcttcatctg ctcttgttca
     1321 agttgcttaa taatgttgct cataatttaa tctcttatcc tgggtaaact gatatctcgg
     1381 gggcttacgc catcccatca tgtttatgtt gctgttgtgc gtgttccgtt ttgaactccg
     1441 ccagcaacct tgcttgctct tcagtcagag ccaggttttc cagaagttca ggtcttctaa
     1501 gccaggtacg gcccagcgac tgtttcaaac gccagcgacg tatctcggca tggttgcccg
     1561 acagtaacac tggcggaact tccatccctt ctaacacctc aggccgcgta tagtgcgggc
     1621 aatccagcaa tccttcagca aaggaatctt ccgttgccga ggcttcatgt cccagtaccc
     1681 ccggaataaa ccgggaaacg gagtcaatca gcgtcattgc tggtaactca ccaccactga
     1741 gaacgtaatc gccgattgac cattcttcgt caatttcggt ttggatcacg cgctcatcta
     1801 taccttcgta gcgaccgcac accagaatca atttttgatt cgttgccaat tcgctgacgc
     1861 ccgcttgatc aagcttgcgt ccctgtggtg acagataaat cacctttgcg ccttcacccg
     1921 ccgcggcttt tgctgcatga atggcgtccc gcaagggttg caccatcatt aacatccccg
     1981 gtccgccgcc gtaaggacga tcgtccacgg tacggtgccg gtcatgcgtg aagtcgcgag
     2041 gactccagct ctggatgctc agcaggccat ttttaactgc ccggccagtt accccgtaat
     2101 cggtaattgc gcggaacatt tcaggaaaca ggctaattat gccaatccac atagcgccgt
     2161 cttttaccgt ttatccggtg gtttaaaaac caggatccca atctacttcg attgaacgag
     2221 tagtgagatc gactttcttg ataacctgcc catcgaggaa cggtacgaga cgttccttga
     2281 taccaaacgc atctttcagg tttgccttaa tgacgagaac gtcattagat ccggtttcca
     2341 tcatatcgac gactttaccg agatcgtagc cttcagtggt tactacctgg cagcccatca
     2401 ggtctttcca gtagtagtca ccctcttcaa gctgaggcaa ctgcgatgaa tccacgacaa
     2461 tttcacaatt cgtcagcagg ttcgccgcat cacgatcgtc aacgcctttc agcttgatga
     2521 tcatgtcctg attgtggtgc ttccagcttt ccagctggac ttgctgccac tgacccgcct
     2581 tctggataaa ccagggctga tagtcaaaaa tgctttcggc gtcttcggtg gaagaaaaca
     2641 ctctgagcca cccacgaata ccgtaagacg aacccatttt tcccaaaacg atgggatcaa
     2701 caggtgcttg cgcggtgagt tgtttgctca tcatgaccac cgtgacagat taagctgctt
     2761 tgtttacttc tttgatcagc gcagcaacgc gatcagaaat agttgcgccc tggccaaccc
     2821 agtgagcgat gcgatccaga tccaggcgag tgccttcttc tttttcgcta gcgattgggt
     2881 tgaagaaacc aacgcgctcg atgaagcgac cgttgcgtgc attacggctg tcagcgacaa
     2941 caacctggta gaacggacgc tttttagcgc cgtgacgtgc taaacgaata gttaccataa
     3001 catcctcttg tgtgaataaa acaaccggac cccatcgagg aacggagtcc ggtgtcatat
     3061 taaaagcccg aaaattttac tgatttctgg agaaaatgca atcagcagtt aataactctg
     3121 tcgcataagg tcatttagcg accaggaaat cccggcggca tcatcccctt catgcttctc
     3181 atcatcttcg ccattccgcc cttcttcatt ttcttcatca tgcgctgcat gtcgtcgaac
     3241 tgtttcagaa gacggttaac gtcctgcacc tgcataccgc aaccggcagc aatacggcgt
     3301 ttacgcgaac ctttgatgat ttctggctta gcgcgctctt tcatcgtcat cgagttgatg
     3361 atggcttcca tacgcaccag cactttatcg tccatctgtg acttgacgtt atccgggatc
     3421 tgccccatgc ccggcagctt gcccatcagg ctggccatgc cgcccatatt tttcatctgg
     3481 cgcagctgct caagaaagtc gttgagatcg aagccgtcac cttttttcag cttgctggct
     3541 aatttctctg cctgcgcgcg gtcaactttg ctttcgatat cttcgatcag cgacagtacg
     3601 tcgcccatgc cgagaatacg cgacgcgatg cggtccggat ggaacggctc cagcgcctca
     3661 gttttctcgc caacaccgag gaacttgatc ggtttgccag tgatgtgacg aatagagagc
     3721 gccgcaccgc cgcgggcatc gccgtccact ttggtcaaca ctacgccggt aagcggtaac
     3781 gcttcattga atgcttttgc cgtattggcc gcatcctgac cggtcatggc gtcaaccaca
     3841 aacagggttt caaccgggtt aatcgacgca tggacttgtt tgatctcgtc catcatcgct
     3901 tcgtcaacgt gcagacgacc agcggtatcc accagcagca cgtcgtagaa tttcagtttg
     3961 gcttctttca gcgccgcgtt aacgatatct accggcttct gaccaacatc agaagggaag
     4021 aaatcaacgc ccacctgctc tgccagcgtc tcaagctgtt tgattgccgc cgggcgataa
     4081 acgtcggcag aaaccaccag cactttcttc ttgtgcttct cgcgcaggaa cttaccgagt
     4141 ttaccaacgc tggttgtttt accggcacct tgcaggcccg ccatcagcac gaccgcaggc
     4201 ggttgcgccg ccaggttcag ggtctggttc tcttcgccca tcgccgcaac cagttcgtta
     4261 cggacgattt tgacgaactc ctgccccggc gtcaggctct tattaacttc atgaccaacc
     4321 gctttctctt ttacgcgatt gataaactca cgcactaccg gcagagcgac atccgcctcc
     4381 agcagcgcca tgcgcacttc gcgcagcgta tcttttacgt tgtcttcagt taggcgtcca
     4441 cggccactga tattgcgcag cgtgcgcgac aaacgatcgg ttaaattatc aaacattgtc
     4501 tctcgcctgg ggtggaaacg gttggccgca atcgcgacac atcatcagta ttttgccgca
     4561 gtataacatg aaggcgtctt tgttgttatg caacggttgg agcagcgttc acctgacgct
     4621 atactgcttc tctttcttat tgctcaaact gttgacatca ctatgcccgt ttttgctctg
     4681 ctcgcgcttg tcgcctactc cgtcagtctt gcgctgattg ttcccggtct gctgcaaaaa
     4741 aacggcggct ggcggcgcat ggctattatt tctgcggtca ttgcgctggt ctgccacgca
     4801 atcgctctgg aagcccgcat cctgcccgac ggtgatagcg gacaaaacct cagcctgctg
     4861 aacgttggtt cattggtcag tttgatgatc tgtacggtaa tgaccattgt ggcttctcgc
     4921 aatcgtggct ggttgctgct acccattgtc tatgcctttg cgcttatcaa cctggcgctg
     4981 gcgaccttca tgcccaatga atacatcacc catctggaag caacgcctgg gatgctggtg
     5041 cacattggct tatcgctctt ttcctatgcc acgctaatta tcgccgccct gtacgcgctg
     5101 caactggcgt ggattgatta ccaactgaag aacaagaagc tggcatttaa ccaggaaatg
     5161 ccgccattga tgagtatcga gcgtaaaatg ttccacatca cgcagattgg cgtggtgctg
     5221 ctaacgctca cgctttgcac tggcctgttc tacatgcaca acctatttag catggaaaat
     5281 atcgacaagg ctgtgctctc tatcgtggcg tggtttgtct atattgtgct gctgtgggga
     5341 cattaccatg aaggatggcg tggacgccgc gtcgtctggt ttaacgttgc gggcgcggta
     5401 attctgacac tggcctactt cggcagccga attgtccagc agttaatcag ctaaacccag
     5461 aaaaggagtt tcccctggaa cacatttcta ctactacgtt gatcattatt ctgatcatca
     5521 tggtggtcat ttcagcctat ttttccgggt ccgaaaccgg aatgatgacc ctcaaccgct
     5581 atcgtctgcg acatatggcg aaacagggta atcgctcggc caaacgcgtc gaaaaattgc
     5641 tgcgtaagcc agaccgcctg ataagcctgg tgttaatcgg caataacctg gtcaatattc
     5701 ttgcctccgc gctcggcact attgttggga tgcgtttgta cggcgatgcg ggcgtggcaa
     5761 ttgcgactgg tgtgctgact tttgtcgtac tggtatttgc tgaggtattg ccgaaaacca
     5821 ttgccgcgct gtacccggaa aaagtcgctt atccgagtag ttttctgctg gctccgctgc
     5881 aaattttgat gatgccgctg gtctggttgc tgaatgctat cacccgtatg ctgatgcgca
     5941 tgatgggtat caaaaccgat atcgtggtta gcggctcttt gagcaaagaa gagttgcgca
     6001 ctatcgtgca cgaatcacgc tcacaaattt cccgtcgcaa tcaggatatg ctgctgtcgg
     6061 tgctcgatct ggaaaaaatg accgttgatg acatcatggt gccgcgcagt gaaattatcg
     6121 gtattgatat caacgatgac tggaaatcga ttctgcgcca actctcccac tcacctcacg
     6181 ggcgcatcgt gctctaccgt gattcgctgg acgacgccat cagtatgctg cgagtacgtg
     6241 aagcctggcg attgatgtcg gagaaaaaag agttcaccaa agaaaccatg ctgcgcgccg
     6301 cggacgagat ctattttgtg ccggaaggta cgccgctcag cacgcagttg gtaaagtttc
     6361 agcgcaacaa aaagaaagtc ggcctggtcg tcaacgagta tggagacatt caggggctgg
     6421 tgacggttga agatattctg gaagagattg tcggcgattt cactacgtcg atgtcgccaa
     6481 cacttgccga agaggtcacg ccgcaaaacg acggttcggt gattatcgat ggcaccgcca
     6541 acgtgcggga aatcaacaaa gcctttaact ggcatctacc ggaagatgat gcccgcacgg
     6601 ttaatggcgt cattcttgag gcactggaag agatccctgt cgcaggcacc cgcgtgcgta
     6661 ttggcgagta cgatatagat attctcgacg tacaggacaa tatgattaag caggtaaaag
     6721 tttttcctgt gaaaccgctg cgcgagagtg tggcggagta acgaaaacgg ccctgcattc
     6781 gcatgcaggg ccgtgaatta ttacgaaaac agaaattacg cttttgcttt cgctacagtt
     6841 accatcgccg cacgaatcgt acgaccattc agcgtataac ccttctgcat aatgcccagt
     6901 acgttacctg gcgcaacgtc atcagattcc accattgcga tagcctgatg cacattcggg
     6961 tccagcggca cgttagtttc ggcgatcact tcaacaccaa acttacgcac aacatccagc
     7021 atcgacttca gcgtcagctc aatgccttca accatcgcag acatatccgg gttagcttta
     7081 tcagccactt ccagcgcacg atccaggcta tcaatcaccg gcagcaattc gttgatgaat
     7141 ttctccagcg caaatttgtg ggctttttca atatccagtt cagtacgtcg acgcaggttt
     7201 tccatttcgg cttttacacg caaaatgccg tcacgttcac gggtctgggc ttcagccagc
     7261 tgagcttcga gattcgcaat tttttcatcg cgcggatcca cctgctcagc agaagcttct
     7321 ggctcaactg cctcaatctc ttcgtgctga tccatgataa tttcttccgg ggcttgcccc
     7381 tcaggcgttt tctgttcttt actactcatg aatttctccg cgtttttttc gcattcatct
     7441 cgctaacttc gcttattatg gggatcagtt tcagggtttc aagggaagca ctcacattgt
     7501 catcaatctt cgcaacaagg acctcggaaa aatgaataat catttcaagt gtattggcat
     7561 tgtgggacac ccacggcacc ccactgcact gacaacacat gaaatgctct accgctggct
     7621 gtgcaaaaaa ggttacgagg tcatcgttga gcaacaaatc gctcacgaac tgcaactgaa
     7681 gaatgtgaaa actggcacgc tcgcggagat tgggcaacta gctgatctcg cggtagtcgt
     7741 tggtggcgac ggtaatatgc tgggcgcggc acgcacactc gcccgttacg atattaaagt
     7801 tattggaatc aaccgtggca acctgggttt cctgactgac cttgaccccg ataacgccca
     7861 gcaacagtta gccgatgtac tggaaggcca ctacatcagc gagaaacgtt ttttgctgga
     7921 agcgcaagtc tgtcagcaag attgccagaa acgcatcagc accgcgataa atgaagtggt
     7981 gcttcatcca ggcaaagtgg cacatatgat tgagttcgaa gtgtatatcg acgagatctt
     8041 tgcgttttct cagcgatctg atggactaat tatttcgacg ccaacaggct ccaccgccta
     8101 ttccctctct gcaggcggtc ctattctgac cccctctctg gatgcgatta ccctggtgcc
     8161 catgttcccg catacgttgt cagcacgacc actggtcata aacagcagca gcacgatccg
     8221 tctgcgtttt tcgcatcgcc gtaacgacct ggaaatcagt tgcgacagcc agatagcact
     8281 gccgattcag gaaggtgaag atgtcctgat tcgtcgctgt gattaccatc tgaatctgat
     8341 tcatccgaaa gattacagtt atttcaacac attaagcacc aagctcggct ggtcaaaaaa
     8401 attattctaa ttttacgcca gcctctttac tgtatataaa accagtttat actgtacgca
     8461 ataacagtaa tggtttttca tacaggaaaa cgactatgtt ggcacaactg accatcagca
     8521 actttgctat cgttcgtgag cttgagattg attttcatag cggcatgacc gtaataactg
     8581 gcgagaccgg cgcgggtaaa tctattgcaa tagatgccct cggtctttgt ctcggtggtc
     8641 gcgctgaagc cgacatggtg cgtaccggcg ctgctcgcgc tgacctgtgc gcccgttttt
     8701 ctctgaaaga tacgccagcg gctctgcgct ggctggaaga aaatcagctt gaagacgggc
     8761 atgaatgttt gcttcgtcgc gtaatcagca gcgatggtcg ctcccgtggt ttcatcaacg
     8821 gtacagctgt tcctctgtca caactgcgcg aactgggtca gttgctgatt cagatccatg
     8881 gtcagcacgc tcatcaatta ctcaccaaac ctgagcacca aaaattcctg cttgatggct
     8941 atgccaatga aacctctcta ctgcaggaaa tgaccgcacg ttatcagttg tggcatcaaa
     9001 gctgccgtga cctcgcgcat catcaacagt taagtcagga acgcgccgcc cgtgcggaac
     9061 tgctgcaata ccaattaaaa gaacttaacg aatttaatcc gcagcccgga gagtttgaac
     9121 aaatcgacga agagtacaaa cgtctggcga acagcggtca attgctgacc accagccaga
     9181 atgcattggc attaatggcc tacggtgaag acgcaaacct gcaaagtcag ctttacacgg
     9241 ctaaacaact ggtgagcgaa ttgattggca tggacagcaa actgtccggc gtacttgata
     9301 tgctggaaga agctaccatc cagattgctg aagccagcga tgaactgcgc cactactgcg
     9361 atcgtctgga tctcgatccc aaccgactat ttgaacttga acagcgcatc tcaaaacaga
     9421 tttcgctggc acgtaaacat cacgtcagcc ctgaggcatt gccacagtat taccagtcgc
     9481 tactggaaga acagcagcaa ctggacgatc aggccgactc acaagaaacg cttgcgctgg
     9541 cggtaacgaa acatcatcag caggcactgg aaatcgcgcg cgcattacac caacaacgcc
     9601 agcaatatgc agaagaactt gcacagctga tcaccgacag tatgcatgcg ctctcaatgc
     9661 cgcatgggca gtttacgatc gatgttaaat ttgacgagca tcacctgggc gctgacggtg
     9721 ccgatcgtat tgagtttcgg gtaaccacca acccaggtca gccaatgcag cctattgcca
     9781 aagtcgcatc cggtggtgaa ttgtcccgca tcgcactggc aacccaggtc atcacggcgc
     9841 gtaaaatgga aaccccggca ctgatttttg atgaagtgga tgtagggatt agcggtccaa
     9901 cagcggcagt tgtcggcaaa ctgctgcgtc aactcggcga atcaactcag gtgatgtgtg
     9961 ttacccacct gccacaagtc gcgggatgtg gtcatcaaca ctattttgtc agcaaagaaa
    10021 ccgatggtgc gatgacagaa acgcatatgc aatccctgaa taaaaaagcg cggttacaag
    10081 agctggcgcg cctgcttggt ggcagtgaag tcacacgtaa tacactggcg aatgcgaaag
    10141 aactgcttgc agcgtaaact tttttcctgc ttcacggtca gagtaaacag caaaacgccg
    10201 taagaccgga aagcaaaagg ttttaaagtg atgaaaggtc tattatcatc ggcatattac
    10261 agatgagcca cgtactgcta gggcccgaaa aggaatcaaa tcactatgcg ctgtaaaacg
    10321 ctgactgctg cagcagcagt actattgatg ttgaccgcag gctgttccac tctggagcga
    10381 gtggtttacc gtcctgacat caaccagggg aactatctga ccgctaacga cgtatccaaa
    10441 atacgtgttg gcatgacgca acaacaagtt gcgtacgcat tgggtacacc gctgatgtcc
    10501 gatccatttg gtacgaatac ctggttctat gtcttccgcc agcaaccagg tcatgaaggt
    10561 gtaacgcagc aaacgctgac gctgaccttt aacagtagcg gtgtgttgac caatattgat
    10621 aacaaacctg cgctgagtgg taactaataa taaagttgct ctcaaagacg ttaaaaaagg
    10681 tgctcaatga gcaccttttt tctgtctgtt atttattcgc tgatttttct gctcgttgcc
    10741 tgcgaagctc tttcggatcg gcaatgagag ggcgataaat ctccacccga tcgccatcat
    10801 gcacactatc gcttagtttt gccggacggc tgtaaatgcc gactttgttt ttagttaaat
    10861 cgatatcggt acgcaattcc agcaagccac tggcgcgaat agcttcttca accgtcgcgc
    10921 cctcctgcag cgtcacgcgc tgcaggtact gcttctcagg tagcgcataa gccacctcaa
    10981 cggcaatttt acctggcact gtaaacctct ttcgcacgaa ccgtaaaagc ctggaccata
    11041 ttagacgcca gctctttaaa cacgcgacca aaggcgagtt caatcaactt attggtaaac
    11101 tcaaagtcga gatgaaactc gatacgacac gcctcctggc tcagcggcgt aaacttccat
    11161 ccaccaatca atttcttgaa cggcccatcc accagattca taagaatact ttggttactg
    11221 gtcaactggt tgcgggtagt aaacgttttg ctgatcccag ccttagagac atctaccgcc
    11281 gcagtcatct gcccaggagt ggactccaga atccgacttc cggtacaacc cggcaaaaac
    11341 tgaggataag actgaacgtc attcactaac tgatacattt gctccgcgct gtagggtacc
    11401 agtgcggtcc ggctaatctg aggcataact atttccatca acaaaaatcc aacaaataat
    11461 atcatttatc ctgctgtaaa aaaaacgcta tcccggcgct gggtaacatc gggttcatgc
    11521 taagatagag ccttgtcccc cgcaggattg atatggggtg ttttcgattt cagattaccg
    11581 atgattcacg acgcttatga cgaagaaaaa agcacataaa cctggttcag cgaccatcgc
    11641 gcttaacaag cgcgcccgtc acgaatactt tatcgaagaa gagttcgaag cgggacttgc
    11701 cctgcaaggc tgggaagtta aatccctgcg cgcaggaaaa gccaatatca gcgacagcta
    11761 cgtccttctg cgtgacggag aggcatttct gtttggcgct aacatcacgc caatggccgt
    11821 ggcctccacg catgtggtgt gcgatcctac ccgtacccgc aagttacttc tcaaccagcg
    11881 cgaactggac tcattgtacg gtcgcgtcaa tcgagaaggc tataccgtag tggcgctctc
    11941 cctgtactgg aaaaatgcct ggtgcaaagt gaaaatcggc gtcgccaaag gtaaaaaaca
    12001 gcacgataaa cgttcagata tcaaagaacg cgaatggcag gtggataaag cacgtatcat
    12061 gaaaaacgcc caccgttaaa cctgcactcc aattattgac cagttcctca ccgcgcctcc
    12121 ctctccggcg gcgcgaatga acatcttatt ggctatcaca tccgacacaa atgttgccat
    12181 cccattgctt aatcgaataa aaatcaggct acatgggtgc taaatcttta acgataacgc
    12241 cattgaggct ggtcatggcg ctcataaatc tggtatactt gccgttacac attggggctg
    12301 attctggatt cgacgggatt tgcgaaaccc aaggtgcatg ccgaggggcg gttggcctcg
    12361 taaaaagccg caaaaaatag tcgcaaacga cgaaaactac gctttagcag cttaataacc
    12421 tgcttagagc cctctctccc tagcctccgc tcttaggacg gggatcaaga gaggtcaaac
    12481 ccaaaagaga tcgcgtggaa gccctgcctg gggttgaagc gttaaaactt aatcaggcta
    12541 gtttgttagt ggcgtgtccg tccgcagctg gcaagcgaat gtaaagactg actaagcatg
    12601 tagtaccgag gacgtaggaa tttcggacgc gggttcaact cccgccagct ccaccaatca
    12661 tgattggacg gtataaggac aacaccaata aaaacaggag gttagcagtc tcagcaggac
    12721 accgaccaga cggtgaagag acataaaagg atacgcaaag gagccgcggc tcttggtgac
    12781 atgaaagccc acagatgtgg gctttttcgt tgatggtcag aacgaccagt tcacaccagc
    12841 taccccgttc cacggggatt ccacaccggc accatggcta taccccaccc caagatgccc
    12901 gcttaacgta ctgctgaatg aggctttaat acctgcctgg tatattccac gtctgcccga
    12961 caaatcattg acgaaattac cgtcactatt cactttcacc cggttatcat cgacaaattc
    13021 tttgcgcaca gccgccttca gccacggctc aacttccata ccgttcccca gacgcatgtt
    13081 gtaactcagc gttgcaccca gttcacgata taaacctgcg ggtatcgact gatttcgatt
    13141 ccatgccatt ggataaatga tattcagggt tatcagcggt gaacccccgt taacgatgca
    13201 tacggcgtca ggttccagtt accatcggta aatcgcatcc cggtttcaat gtgaccgccc
    13261 agcccgttgc tgcgataact gccgttggtg gctccaccgc tgccaaccgt ggagtcgcga
    13321 gcttcggtac ctgctaatac caataaatta ccgccatttt ccagcaacat attggtcgct
    13381 aaattgccgg aaatggaaaa agtgccgtac tggtgagtac cgctgatttc aataccgtta
    13441 gccgtgctcg tctggagagc ggcaccgctg ttctggacga tatctgtcgc tttgccatta
    13501 tcgttaactg tcagcgtacc gccttcattg atctttgttt ttattgcctc tccgttagct
    13561 gaaactgttt gtattccgcc gtcgttaatc gttgtctcat tcgccacccc ctcgacgatt
    13621 tgttcgccgc cggtgagcgt cgtgcctgtc gcagtggctt ttgttttgac gatctcccgt
    13681 ccgcccgtat tgacctgtgt tttgtcagaa gaggtgtctg actccacggt taacacgccg
    13741 ccatttgcca gcaggatatt gttcgccgca ccctgctcga tgctgaacgc gacgccatcc
    13801 gcgcgtgttc ctgtgacccg cgtcgccctg gtggttgcaa ccaaagcgcc ctggctactc
    13861 tgctgtatcc ccgttgcgct gcctttctcc cgcacatcga gtgtgccgcc gtcattaagc
    13921 accgagtttt cagccagacc gccctcatta actacctgtg aacccccatt aataatggaa
    13981 ccttccgctg tcccgtttgc cataatttgt tgtaggccag agacgatatc ggtattgatt
    14041 gccttaccat aattctgaac ggtttgcgtg ccaccattga tgtgtgtttt ctctgttgac
    14101 ccaccatcaa caatttgttc accactttcg atatttgcct cagtggctaa tccatatacc
    14161 gtttgcttgc cacctttgat atttgcttta tcagaagtgg cactggcata tattgtttgg
    14221 gtgccagcac tattgagtac agtgccaaca tcttttccat aaacatccat tttgccgttg
    14281 gcattaataa tcgtatcaac tgcccgggaa ccagtgacga ctgttaatga gccagcgttt
    14341 tccagcacta catttttagc ttctgaattc ctgatgtaga aagcatcacc ataactgttg
    14401 gttcctttga taagggttcc ggaagttgtg gaagcaatta atgcgccgcc ggattgttgc
    14461 tcaacatgct tagcctcacc accgtcctga acctccagaa cgccaccatt attaagtctg
    14521 gtggtatctg ttttagcctc cttctggaca atcagcttac cgccagcatc aacggtagta
    14581 tttttcgccg aggttttagc cactaccgtc agttcgccgg tattttccag cacaacataa
    14641 ttagcctccc ctccgggtaa tagtgaagtg agagagcttg ttgtatcctt cgatatcagt
    14701 ccctgcgccc gtgttggcaa ctaaagcact gcccgtctcc tggttaaccc catgtgctat
    14761 accgccggta tagacaatca gcgagcctcc ggcgctaata ttgctgccag ttgccgtacc
    14821 atctttctca acaacctgcc ggctcccggt ggaaatattt gtcgtgtcag ctttcccgcc
    14881 gctcttgata ttttgtgttc cgctcttgat attttgtgtt ccgctgttga tattggtacc
    14941 cgtggcaata ccatgattat taatattctg tgtaccacca ttgattatgg tattggtcgc
    15001 gtttccggta acatccatta ccccgccgtt atctattcgg gtcgcatcag ctttagcatt
    15061 cgttaaaact gacattgttc ctttatcttt aataatcgtc ttatttgccg aaccatatgc
    15121 gtttatgtct aaatgaccac c
//
LOCUS       DAZPEB010000095        15140 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZPEB010000095 DAZPEB010000000
VERSION     DAZPEB010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15140
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.096"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            76..1365
                     /gene="hpaG"
                     /locus_tag="ACHURJ_002488"
                     /pseudo
     CDS             76..1365
                     /gene="hpaG"
                     /locus_tag="ACHURJ_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008704 -
                     5-carboxymethyl-2-hydroxymuconate delta-isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018800 -
                     5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
     gene            1362..2821
                     /gene="hpaE"
                     /locus_tag="ACHURJ_002489"
                     /pseudo
     CDS             1362..2821
                     /gene="hpaE"
                     /locus_tag="ACHURJ_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
     gene            2823..3674
                     /gene="hpaD"
                     /locus_tag="ACHURJ_002490"
     CDS             2823..3674
                     /gene="hpaD"
                     /locus_tag="ACHURJ_002490"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HFR1281392.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            3684..4064
                     /locus_tag="ACHURJ_002491"
     CDS             3684..4064
                     /locus_tag="ACHURJ_002491"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HFR1281393.1"
                     /translation="MPHFIVECSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQRDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            4175..4978
                     /gene="hpaH"
                     /locus_tag="ACHURJ_002492"
     CDS             4175..4978
                     /gene="hpaH"
                     /locus_tag="ACHURJ_002492"
                     /EC_number="4.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HFR1281394.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            4989..5777
                     /gene="hpaI"
                     /locus_tag="ACHURJ_002493"
     CDS             4989..5777
                     /gene="hpaI"
                     /locus_tag="ACHURJ_002493"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HFR1281395.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLSDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVHATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIRESGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAVKPGVY"
     gene            5952..7328
                     /gene="hpaX"
                     /locus_tag="ACHURJ_002494"
     CDS             5952..7328
                     /gene="hpaX"
                     /locus_tag="ACHURJ_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901241 - 4-hydroxyphenylacetate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1900754 - 4-hydroxyphenylacetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HFR1281396.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLIVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGLLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATP"
     gene            7338..8224
                     /gene="hpaA"
                     /locus_tag="ACHURJ_002495"
                     /pseudo
     CDS             7338..8224
                     /gene="hpaA"
                     /locus_tag="ACHURJ_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224927.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
     gene            8475..10037
                     /gene="hpaB"
                     /locus_tag="ACHURJ_002496"
     CDS             8475..10037
                     /gene="hpaB"
                     /locus_tag="ACHURJ_002496"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HFR1281397.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSANDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDADGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQHIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            10055..10567
                     /locus_tag="ACHURJ_002497"
     CDS             10055..10567
                     /locus_tag="ACHURJ_002497"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HFR1281398.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            10960..13110
                     /gene="btsT"
                     /locus_tag="ACHURJ_002498"
     CDS             10960..13110
                     /gene="btsT"
                     /locus_tag="ACHURJ_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFR1281399.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            13241..13444
                     /locus_tag="ACHURJ_002499"
     CDS             13241..13444
                     /locus_tag="ACHURJ_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFR1281400.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            13455..14411
                     /gene="yjiA"
                     /locus_tag="ACHURJ_002500"
     CDS             13455..14411
                     /gene="yjiA"
                     /locus_tag="ACHURJ_002500"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFR1281401.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(14513..>15140)
                     /locus_tag="ACHURJ_002501"
     CDS             complement(14513..>15140)
                     /locus_tag="ACHURJ_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HFR1281402.1"
                     /translation="PGSAILNVWLTSMLKRTVVAAVPMPFDKWYSASGYETTQDGPTG
                     SLNISVGAKILYEAVQGDKSPIPQAVDLFAGKPQQEVVLAALEDTWETLSKRYGNNVS
                     NWKTPAMALTFRANNFFDVPQAAAEETRHQAEYQNRGTENDMIVFSPTTSDRPVLAWD
                     VVAPGQSGFIAPDGTVDQHYEDQLKMYENFGRKSLWLTKQDVEAHKES"
BASE COUNT         3491 a         4192 c         4127 g         3330 t
ORIGIN      
        1 ccaaactacg caatcgggaa taaaagcaat cattaacata ttaatgatta agataatcga
       61 atccgaggag tggtaatgaa aggcactatc ttcgccgtag cgttgaacca tcgcagccaa
      121 cttgatgcat ggcaggaagc gttccagcaa tccccctaca aagccccgcc taaaactgcg
      181 gtctggttta ttaaaccgcg caatacggtg attggttgcg gtgaaccgat tccctttcca
      241 cagggtgaaa aggtactgag cggtgcgact gttgcgctga ttgtgggaaa aacggcgacg
      301 aaagtacgtg aagaagatgc ggcagagtac atcgccggat atgcgctggc taacgacgtc
      361 agcctgccgg aagagagctt ttaccgcccg gcaatcaaag caaaatgtcg tgatggattc
      421 tgccccattg gcgaaaccgt ggctctcagc aatgtcgata atctgaccat ctataccgag
      481 atcaacgggc gtcctgccga tcactggaat accgccgatt tacaacgtaa cgccgcgcag
      541 ttgctgagcg ccctgagcga atttgccacg ctgaatccag gcgacgccat tctgctcggc
      601 acgccacagg cgcgcgtgga aatacagcca ggcgatcgcg ttcgtgttct cgcagaaggt
      661 ttcccgccgc tggaaaatcc ggtagtggac gaacgtgaag tgaccacgcg caagagcttc
      721 ccaacgcagc cacacccgca cggcacgctg tttgccctcg gcctgaacta cgccgaccac
      781 gccagcgaac tggaatttaa gccaccggaa gaaccgctgg tattcctgaa agcgccaaat
      841 accctcactg gcgataacca gacctccgtg cgtccaaaca atattgaata catgcactat
      901 gaagcggagc tggtggtagt tattggcaaa caggcgcgta acgtcagcga agccgatgcc
      961 atggattatg tcgcgggcta caccgtgtgt aacgactacg ccattcgtga ctatctggaa
     1021 aactactacc gccctaacct gcgggtaaaa agccgcgacg gactgacgcc gatgctttca
     1081 accatcgtgc cgaaagaggc gatcccggac ccgcataatc tgacccttcg caccttcgtc
     1141 aacggcgagt tacgccagta aggcaccacc gccgatctga tcttcagcgt gcccttcctg
     1201 atcgcctact taagtgaatt tatgaccctg aatccgggcg acatgatcgc caccggcaca
     1261 cccaaaggct tatctgacgt ggtgcctggc gatgaagtag tggtggaagt agaaggcgtg
     1321 ggccgcctgg tgaaccgaat tgtgagtgag gatacagcga aatgaaaaaa gtaaatcatt
     1381 ggatcaacgg caaaaatgtt gcaggtaacg actacttcca gaccaccaat ccggcaacgg
     1441 gtgaagtgct ggcggatgtg gcctctggcg gtgaagcgga gatcaatcag gcggtagcgg
     1501 cagcgaaaga ggcgttcccg aaatgggcca atctgccgat gaaagagcgt gcgcgcctga
     1561 tgcgccgtct gggcgatctg atcgaccaga acgtgccaga gatcgccgcg atggaaaccg
     1621 cggacaccgg cctgccgatc catcagacca aaaatgtgtt gatcccacgc gcttcccaca
     1681 actttgaatt tttcgcggaa gtctgccagc agatgaacgg caagacctat ccggttgacg
     1741 acaagatgct caactacacg ctggtgcagc cggtgggcgt ttgtgcgctg gtatcgccgt
     1801 ggaacgtacc gtttatgacc gccacatgga aggtcgcgcc gtgtctggcg ctgggcaata
     1861 ccgcggtact gaaaatgtcg gaactctccc cgctgaccgc ctgggtgagc tggcgctgga
     1921 agccggtatt ccggcaggcg tgctgaacgt ggtacagggc tacggcgcaa ccgcagggga
     1981 tgcgctggtt cgtgatcatg acgtacgtgc cgtgtcgttc accggcggta cggccaccgg
     2041 gcgcaacatc atgaaaaacg ccgggctgaa aaaatactcc atggaactgg gcggtaaatc
     2101 gccggtgctg atttttgaag atgccgatat tgaacgcgcg ctggacgccg ccctgttcac
     2161 catcttctcg atcaacggcg aacgctgcac cgccggttca cgcatcttta ttcagcaaag
     2221 catctacccg gaattcgtta aacgctttgc cgaacgcgcc aaccgtctgc gcgtgggcga
     2281 tccgaacgat ccgaataccc aggttggcgc gcttatcagc cagcagcact gggaaaaagt
     2341 ctccggctat atccgtctcg gcattgaaga aggcgccacc ctgctggcgg gcggcccgga
     2401 taaaccgtct gacctgcctg cacacctgaa aggcggcaac ttcctgcgcc caacggtgct
     2461 ggcagacgtt gataaccgta tgcgagtcgc ccaagaagag attttcgggc cggtcgcctg
     2521 cctgctgccg tttaaagacg aagctgaagg cttacgcctg gcaaacgacg tggagtacgg
     2581 cctcgcgtcg tacatctgga cacaggatgt cagcaaagtg ttacgcctgg cgcgtggcat
     2641 tgaagctggc atggtgttcg tcaacaccca gaacgtgcgt gacctgcgcc agccatttgg
     2701 cggcgtaaaa gcctccggca ccgggcgtga aggcggtgag tacagcttcg aagtgttcgc
     2761 ggaaatgaag aacgtctgca tttccatggg cgaccatcca attccgaaat ggggagtctg
     2821 atatgggtaa gttagcgtta gccgccaaga tcacgcacgt accgtcgatg tatctctctg
     2881 aactgccagg gaaaaaccac ggttgccgcc agggcgcgat cgacgggcat aaagagatca
     2941 gcaagcgttg ccgggaaatg ggcgtcgata ccattatcgt tttcgatacc cactggctgg
     3001 tcaacagtgc ttatcacatc aactgtgcag accattttga aggcgtctac accagcaacg
     3061 agctgccgca ttttatccgc gatatgacct acaactacga gggcaacccg gagttggggc
     3121 agcttattgc cgatgaagcc ttaaagctcg gcgtgcgggc aaaagcgcac aacattccca
     3181 gcctgaaact ggaatacggc acgctggtgc cgatgcgcta catgaatgaa gacaagcact
     3241 tcaaagtggt ctccatttcg gctttctgca cggttcacga ttttgccgac agccgcaagc
     3301 tgggcgaagc gattctgaaa gcgatcgaac agtacgacgg caccgtggcg gtccttgcca
     3361 gcggttcgtt atcgcaccgc tttattgacg atcagcgtgc agaagaaggg atgaacagct
     3421 acacccgcga gttcgaccgc cagatggacg agcgcgtggt gaagttgtgg cgcgaaggcc
     3481 agttcaaaga gttctgcaat atgctgccgg agtacgccga ctactgctac ggcgaaggca
     3541 atatgcacga cacggtgatg ctgctgggga tgctcggctg ggataaatac gacggcaagg
     3601 tggagtttat caccgagcta ttcccaagct ctggcaccgg tcaggttaac gctgttttcc
     3661 cgcttcccgc gtaaggacgt tttatgccgc actttatcgt tgaatgcagt gacaacatcc
     3721 gcgaagaagc cgacctgccg gggttgttcg ccaaagtgaa tccgacgctg gcagccacgg
     3781 gtatttttcc gctggcgggt attcgcagcc gcgtgcattg ggtcgatacc tggcagatgg
     3841 ccgacgggca gcgtgattat gccttcgtgc atatgacgtt gaaaatcggc gcaggtcgca
     3901 gcctggaaag ccgtcagcag gcgggtgaaa tgctgtttga actgattaaa acgcacttcg
     3961 ccgccctgat ggagagccgc ctgctggcgt tgtcgtttga gattgaagag ctgcatccga
     4021 cgctgaattt taaacaaaac aacgtgcacg cattgtttaa gtagcgcgca gattgcccgg
     4081 tggcgctgcg cttaccgggc ctacaaaacc ccaaaccgta caccgtaggc cggataaggc
     4141 gcagccgcat ccggcaatgc cacaggatat cgctatgttc gataaacaca cccacaccct
     4201 gatcgcccag cgtctggatc aggcagaaaa acagcgcgaa cagatccgcg cgatctcgct
     4261 ggattaccca gagatcacca tcgaagacgc ttacgcggtg cagcgcgaat gggttcgcct
     4321 gaaaatcgcc gaaggccgta cgctgaaagg ccacaaaatc ggtctgacct caaaagcaat
     4381 gcaggccagc tcgcagatca gcgaaccaga ttacggtgcc ctgctggatg acatgttctt
     4441 ccacgatggc agcgatatcc cgaccgatcg ctttatcgtg ccgcgcattg aagtggagct
     4501 ggctttcgtg ctggcaaaac cgctgcgtgg gccaaactgc acgctgttcg acgtctacaa
     4561 cgccacggac tacgtgatcc cggcgctgga gctgatcgac gctcgctgcc acaacatcga
     4621 tccggaaacc cagcgcccgc gcaaagtgtt cgacaccatt tctgataacg ccgccaacgc
     4681 cggggtgatc ctcggtggtc gtccgatcaa accagacgaa ctggatctgc gttggatctc
     4741 cgccctgatg tatcgcaatg gcgtgattga agaaaccggc gtcgccgctg gcgtgctgaa
     4801 tcatccggca aacggcgtgg cctggctggc gaacaaactc gcaccgtatg acgtacaact
     4861 ggaagccggg caaatcattc tcggtggttc gttcacccgc ccggttccgg cgcgtaaggg
     4921 cgacaccttc cacgtcgatt acggcaacat gggttccatt agctgccgct ttgtttaagg
     4981 agagaacgat ggaaaacagc tttaaagcgg cgctgaaagc aggccgtccg cagatcggat
     5041 tatggctggg gctgagtagc agctacagcg cagagttact ggccggagca ggattcgact
     5101 ggttgttgag cgacggtgag cacgcgccga ataacgtgca aaccgtgctc acccagctac
     5161 aggcgattgc gccctacccc agccagccgg tagtacgtcc gtcgtggaac gatccggtgc
     5221 aaatcaaaca actgctggac gtcggcacac aaaccttgct ggtgccgatg gtacaaaacg
     5281 ctgacgaagc ccgtgaagcg gtacacgcca cccgttatcc ccccgccggt attcgcggtg
     5341 tgggcagtgc gctggcccgc gcctcgcgct ggaatcgtat tcctgattac ctgcaaaaag
     5401 ccaacgatca aatgtgcgtg ctggtgcaga tcgaaacgcg tgaggcaatg aagaacttac
     5461 cgcagattct ggacgtggaa ggcgtcgacg gcgtgtttat cggcccggcg gatctgagcg
     5521 ccgatatggg ttatgccggt aatccgcagc acccggaagt acaggccgcc attgagcagg
     5581 cgatcgtgca gatccgtgaa tcgggcaaag cgccggggat cctgatcgcc aatgagcaac
     5641 tggcaaaacg ctatctggaa ctgggcgcgc tgtttgtcgc cgtcggcgtt gacaccaccc
     5701 tgctcgcccg cgccgctgaa gcgctggcag cacggtttgg tgcgcaggcc accgccgtga
     5761 agcccggcgt gtattaatgc cggatgcgct gcacttatcc ggcctacact cgcaccaaac
     5821 cgtaggccgg ataaggcgtt tacgccgcat ccggcaaaaa gccgtaccaa atcgcggacc
     5881 ggataaggcg ttacgccgca tccgccataa accttgtcgt accctacaaa aatcccatta
     5941 gaggaagaaa aatgagcgac acctcacctg ccataccgga gagtatcgat ccggcgaatc
     6001 agcataaagc gctgactgcc ggacaacagg cggttattaa gaagctattt cgccgcctga
     6061 tcgtctttct gttcgtgctg tttatcttct cgttccttga tcgcatcaac atcggctttg
     6121 ccggactcac gatgggacgc gacctcggtc tgagcgccac catgtttggc ctcgctacca
     6181 ccctgttcta tgccgcttat gtcatcttcg gcattcccag caacattatg ctgagtattg
     6241 tcggtgcgcg gcgctggatc gccaccatca tggtgctctg gggcatcgcc tctactgcca
     6301 ccatgtttgc cactggcccc accagcttgt acgtactgcg tatactggtt ggcattaccg
     6361 aagccggctt tctgcctggt attctgctgt atttaacctt ctggtttccg gcctatttcc
     6421 gcgcccgtgc caacgccttg tttatggtag caatgccggt aacgacagcg ttgggctcga
     6481 tcgtttccgg ctacattttg tcgctggatg gcgtcatggc attaaaaggc tggcagtggc
     6541 tgtttttgct ggaaggcttc ccgtcggtat tactcggcgt catggtgtgg ttctggcttg
     6601 atgactcacc ggacaaagct aagtggctga cgaaagaaga caaaaaatgc ctgcaagaga
     6661 tgatggataa cgatcgtctg acgctggttc agccagaggg agccatcagc caccacgcca
     6721 tgcaacaacg cagtatgtgg cgggagatct tcactccggt ggtgatgatg tataccctgg
     6781 cgtatttctg cctgaccaac acacttagtg cgatcagcat ctggacaccg cagatcctgc
     6841 aaagttttaa tcagggcagc agtaatatca ccatcggcct gctggccgcc gtaccgcaga
     6901 tttgtaccat tctcgggatg gtctactgga gccgtcactc agatcgccgc caggaacgaa
     6961 ggcatcacac cgcccttcct tatttgttcg ctgccgcagg ttggttactg gcttcggcaa
     7021 ctgatcacaa catgatccag atgctgggga tcattatggc ttcgaccgga tcattcagcg
     7081 caatggcgat tttctggaca acaccggatc agtccatcag cctgcgggca cgagcgatcg
     7141 gtattgcggt gatcaacgcc actggcaaca ttggttcagc gttaagtccg tttatgatcg
     7201 gctggttgaa agatttgacc ggcagcttta acagtggatt gtggtttgtt gccgcgctgc
     7261 tggtgattgg tgcggggatt atctgggcga ttccaatgca gtcctcccgt ccgcgagcga
     7321 ccccgtaagg aacgacgatg tgtgaccgtc agattgccaa tattgatatc agcaaagagt
     7381 acgatgaaag cctgggcacg gacgatgtgc attatcagtc gttcgcccgc atggcggcat
     7441 tttttggccg ccatatgctg ccacatcgcc acgaacagta ctttcagatg catttcctca
     7501 atagcggaca gattgagcta cagcttgacg atcatcgcta ctcggtggaa gcgcccctgt
     7561 ttgtcctgac gccgccgtca gtacctcatg cgtttattac ggagtctgac gccgacggtc
     7621 atgtgttgac ggtacgggaa gatctgatct ggcccctgct ggaagttctt tatccgggca
     7681 ctcgggaaac cttcggcctg ccgggggcct gtcactggca gataaacccg acgaactggc
     7741 ggcgctggaa cactattggc aactgataga gcgggaatcg gtggaacaac tgcctggacg
     7801 ggaacacacc ctgacgttac tggcacaggc agtgttcacc ctactgctgc gtaacgcaaa
     7861 actcgacgac catgccgcca gcgggatgcg cggagaatta aaactgttcc agcgttttaa
     7921 tatgctgatt gaaagccatt ttcatcagca ctggacagta ccggattacg ctaacgaact
     7981 gcatatcacc gaatcacgac tcacggacat ctgccgtcgc tttgccaacc gtccgccaaa
     8041 acggttgatt ttcgacaggc agctgcgaga agccaagcgg ctgctgctgt tttctgataa
     8101 cgccgtgaac aatattgcct ggcaactcgg ttttaaggat ccggcttatt ttgcgcgctt
     8161 ttttaatcgc ttagtcggtt gctcgcccag tgcttatcgc gccaaaaaag tacctgtgac
     8221 gtgaaaatcc cttagcctta acgggaaacc aggcaccacc tgctattccc ctttctccgc
     8281 ctggagcaag gggatttagt aacgtcaaaa atcgctaaaa gcgacctcga tcacaaaacc
     8341 gtacccgacc tgaaaagtac cagccattgt cccaaaagtc tcttcccgta atcgcccctg
     8401 caagctttaa ttgaataaca aaaacaacac acaaaattaa caatcgtatt ccgattaata
     8461 ctgtagaggt cgacatgaaa ccagaagatt tccgcgccag tacccaacgt ccgttcaccg
     8521 gggaagagta tctgaaaagc ctgcaggatg gtcgcgagat ctatatctat ggcgagcgag
     8581 tgaaagacgt cactactcat ccggcatttc gtaatgcggc agcgtctgtt gcccaactgt
     8641 acgacgcgct acacaaaccg gagatgcagg actctctgtg ctggaacacc gacaccggca
     8701 gcggcggcta tacccataaa ttcttccgcg tggcgaaaag tgccaacgac ctgcgccagc
     8761 aacgcgatgc catcgctgag tggtcacgcc tgagctatgg ctggatgggc cgtaccccag
     8821 actacaaagc tgctttcggt tgcgcactgg gcgcgaatcc gggcttttac ggtcagttcg
     8881 agcagaacgc ccgtaactgg tacacccgta ttcaggaaac tggcctctac tttaaccacg
     8941 cgattgttaa cccaccgatc gatcgtcatt tgccgaccga taaagtaaaa gacgtttaca
     9001 tcaagctgga aaaagagact gacgccggga ttatcgtcag cggtgcgaaa gtggttgcca
     9061 ccaactcggc gctgactcac tacaacatga ttggcttcgg ctcggcacaa gtgatgggcg
     9121 aaaacccgga cttcgcactg atgttcgttg cgccaatgga tgccgatggc gtaaaattaa
     9181 tctctcgcgc ctcttatgag atggtcgcgg gtgctaccgg ctcgccatac gactacccgc
     9241 tctccagccg cttcgatgag aacgatgcga ttctggtgat ggataacgtg ctgatcccat
     9301 gggaaaacgt actgatctac cgcgatttcg atcgctgccg tcgctggaca atggaaggcg
     9361 gttttgcccg tatgtatccg ctgcaagcct gtgtgcgcct ggcagtgaaa ttagacttca
     9421 ttacagcact gctgaaaaaa tcactcgaat gtaccggcac cctggagttc cgtggtgtac
     9481 aggccgatct cggtgaagtg gtggcgtggc gcaacacctt ctgggcattg agtgactcga
     9541 tgtgttctga agcgacgccg tgggtcaacg gggcttattt accggatcat gccgcactgc
     9601 aaacctatcg cgtactggca ccaatggcct acgcgaagat caaaaacatt atcgaacgca
     9661 acgttaccag tggcctgatc tatctccctt ccagtgcccg tgacctgaac aatccgcaga
     9721 tcgaccagta tctggcgaag tatgtgcgcg gttcgaatgg tatggatcac gtccagcaca
     9781 tcaagatcct caaactgatg tgggacgcca ttggcagcga gtttggtggt cgccacgaac
     9841 tgtatgaaat caactactcc ggtagccagg atgagattcg cctgcagtgt ctgcgccagg
     9901 cacaaagctc cggcaatatg gacaagatga tggcgatggt tgatcgctgc ctgtcggaat
     9961 acgaccagaa cggctggact gtgccgcact tgcacaacaa cgacgatatc aacatgctgg
    10021 ataagctgct gaaataacgc agcaggaggt taagatgcaa ttagatgaac agcgcctgcg
    10081 ctttcgtgac gcgatggcca gcctgtcggc agcggtaaat attatcacca ccgagggcga
    10141 cgccggacaa tgcgggatta cggcaacggc cgtctgctcg gtcacggata caccaccatc
    10201 gctgatggtg tgcattaacg ccaacagtgc gatgaacccg gtttttcagg gcaacggtaa
    10261 gttgtgcgtc aacgtcctca accatgagca ggaactgatg gcacgccact tcgcgggcat
    10321 gacaggcatg gcgatggaag agcgttttag cctctcatgc tggcaaaaag gtccgctggc
    10381 gcagccggtg ctgaaaggtt cgctggccag tcttgaaggt gagatccgcg atgtgcaggc
    10441 aattggcaca catctggtgt atctggtgga gattaaaaac atcatcctca gtgcagaagg
    10501 tcacggactt atctacttta aacgccgttt ccatccagtg atgctggaaa tggaagctgc
    10561 gatttaagct ccagcccctt cacgtattcg tcgaaggggc atttttattg tgatctcaat
    10621 cacatatcca cccgatgaat aattcttgcc caaccgctca ccgccattcc tgaccgctta
    10681 cgtccctaaa ataaccactc agttatttac cttactttac gcgcgcgtaa ctctggcaac
    10741 atcactgcag gatagcggtc aatttacctc ctcaaacgca acgcaaacct agaacggctt
    10801 cggcctatga ttaacaaatt actctatgaa ttttggtttg caggaaggca gcaagtgagt
    10861 gaatccccgg gagcttacaa cagtaagtga cagaggtgaa cgaacgcagc tgccgcacct
    10921 gtaagccaaa agacgacgag taaaatgcca ggttttacta tggatacgaa aaaactattc
    10981 aagcacatac cctgggtgat tctcggaatc atcggtgcat tctgcctcgc ggtagttgca
    11041 ttacgtcggg gggagcacgt cagcgccctg tggatcgtgg tcgcctctgt gtcggtgtat
    11101 ctggtggcgt atcgctacta cagtctgtac atcgcccaga aggtgatgaa actcgacccc
    11161 acgcgcgcga cgcctgcggt tattaacaac gacggtctga actacgttcc gaccaaccgt
    11221 tacgtgttgt ttggtcacca cttcgccgct atcgccggtg ccggtccgct ggttggtccg
    11281 gtgctcgccg cgcagatggg ctacctgcct ggcacgctgt ggctgctggc gggggtagta
    11341 ctggccggtg cggttcagga ctttatggtg ctgtttatct cctctcgccg taacggcgca
    11401 tctctcggtg agatgatcaa agaagagatg ggaccagtac cggggactat cgcgctgttt
    11461 ggctgtttct taatcatgat catcatcctc gccgttctgg cgctgattgt ggttaaagcc
    11521 ctggccgaaa gtccgtgggg tgtcttcacc gtttgctcaa ccgtaccgat tgcgctgttt
    11581 atgggtatct acatgcgctt tatccgtccg gggcgtgtag gtgaagtttc tgttattggt
    11641 atcgtgctgc tggttgcctc tatctatttc ggtggcgtga ttgctcacga tccgtactgg
    11701 ggtccggcac tgacctttaa agacaccacc attaccttcg cgctgattgg ctatgcgttt
    11761 gtttccgcac tgctgccggt gtggctgatc ctcgcaccgc gcgactatct ggcaaccttc
    11821 ctgaaaatcg gcgttatcgt cggcctggcg ctgggtatcg tggtactgaa cccggaactg
    11881 aaaatgcctg caatgaccca gtacattgac ggtactggcc cgctgtggaa aggcgctctg
    11941 ttcccgttcc tgttcatcac catcgcctgt ggtgcggtat ctggcttcca cgcgctgatc
    12001 tcttccggta cgacgccgaa attgctggct aacgaaaccg acgcgcgttt cattggctac
    12061 ggcgcaatgc tgatggaatc cttcgtggcg attatggcgc tggttgctgc gtccatcatc
    12121 gaaccgggtc tttacttcgc gatgaatacc ccgcctgctg gccttggcat caccatgcct
    12181 aacctgcatg aaatgggtgg cgagaacgcg ccgatcatca tggcgcagct gaaagacgtt
    12241 accgcacacg cggcggcgac cgtcagctcc tggggcttcg tgatttcgcc agagcagatc
    12301 ctgcaaaccg cgaaagacat tggtgagcct tctgtcctga accgtgcagg tggcgcgccg
    12361 acgctggcgg taggtatcgc tcacgtgttc cacaaagtgc tgccgatggc tgacatgggc
    12421 ttctggtatc acttcggtat tctgttcgaa gccctgttca tcctgaccgc gctggatgcg
    12481 ggtactcgtt ctggccgctt tatgctgcaa gacctgctgg gtaacttcat cccgttcctg
    12541 aaaaaaaccg actctctggt tgccggtatc atcggtactg cgggctgtgt gggtctgtgg
    12601 ggctacctgc tgtatcaggg cgtggtcgat ccgctgggcg gcgttaagag cctgtggccg
    12661 ctgttcggta tttccaacca gatgctggca gccgtagcgc tggtactggg caccgttgtg
    12721 ctgattaaga tgaagcgcac ccaatacatc tgggtaactg ttgttccggc tgtatggctg
    12781 cttatctgca ccacctgggc gctgggtctg aaactgttca gcaccaaccc gcagatggaa
    12841 ggcttcttct acatggcaag ccagtacaaa gagaagattg ctaacggtac tgacctgacg
    12901 gcgcagcaga ttgccaacat gaaccacatc gttgtgaaca actacaccaa cgcaggcctg
    12961 agtattctgt tcctgattgt ggtgtacagc atcatcttct acggtttcaa aacctggctt
    13021 gcggtgcgta acagcgacaa acgtactgac aaagaaactc cgtacgttcc aatcccggaa
    13081 ggcggcgtga agatctcttc gcaccactaa ccggttgccg gatgcgctgc gcgtatccgg
    13141 cctacaaagt gtctatcgta ggccggataa ggcgtttacg ccgcatccgg caaatcccta
    13201 gcccggacct tccgggcttt acgcatatct ggaaggcaaa atgtttggta acttaggaca
    13261 ggcaaaaaaa tatctcggcc aggcggcgaa gatgttgatt ggcattccag actacgacaa
    13321 ctacgtcgag catatgaaga ccaaccatcc cgacaagccg tacatgagct atgaagaatt
    13381 cttccgcgaa cgccagaacg cgcgctacgg cggcgatggt aaaggcggta tgcgctgttg
    13441 ttaatggaga gagcatgaac ccgattgcag ttaccctact caccggtttt ttaggcgcgg
    13501 gcaaaaccac cctgctgcgc catattctta acgaacaaca cggctacaag attgccgtga
    13561 ttgaaaacga attcggcgaa gtctccgttg atgatcaact gattggcgac cgcgccacgc
    13621 agatcaaaac gctgaccaac ggctgcatct gctgttcgcg ctccaacgag ctggaagacg
    13681 cactactcga cctgctggat aatctcgaca agggcaatat tcagttcgac cgtctggtca
    13741 ttgaatgcac cggcatggcc gatcccggcc cgattattca gaccttcttc tcccatgaga
    13801 ttttatgcca gcgctacctg ctggacgggg tgattgcgct ggtggatgcg gtacatgccg
    13861 atgagcagat gaaccagttc accatcgccc agtcccaggt tggctacgcc gaccgtattc
    13921 tgctgacgaa aaccgacgtc gcaggcgaag cagagaaact gcgtgaacgc ctggcgcgca
    13981 tcaacgcccg cgcaccggtc tacaccgtta cccacggcga tatcgacctg ggccttctgt
    14041 tcaacaccaa cggttttatg ctggaagaaa acgtcgtcag caccaaaccg cgtttccact
    14101 ttatcgcgga caaacaaaac gatatttcgt cgattgtggt ggaactggat tacccggtag
    14161 atatcagcga agtttcccgg gtgatggaaa acctgctgct ggaatcggcg gataaactgc
    14221 tgcgttacaa agggatgctg tggattgacg gcgaacctaa ccgcctgctg ttccagggcg
    14281 tccagcgcct ctacagcgcc gactgggacc gcccgtgggg cgatgaaaaa ccgcatagca
    14341 cgatggtgtt tatcgggatt cagttgccgg aggatgagat tcgggcggcg tttgcgggac
    14401 tcaaaaagta accagccctc caacatcaca atacctgaga ctttatcacc ctcatattcc
    14461 tccagaatat gagggcattc gggcttaatt atctctgaac gtgcaacact tcctacgact
    14521 ccttatgcgc ctccacatcc tgcttcgtta accagagcga cttacgacca aaattttcgt
    14581 acattttcag ctgatcttca tagtgctgat caactgttcc atcgggagca ataaacccac
    14641 tctgaccggg tgcgaccaca tcccaggcaa gcaccggacg atcgcttgtc gttggtgaga
    14701 aaacaatcat atcgttttct gttccacggt tttgatactc cgcctgatga cgcgtttctt
    14761 ccgctgcggc ctgcggtaca tcaaagaaat tatttgcccg gaacgttaag gccattgcag
    14821 gtgttttcca gttactcaca ttattgccat agcgtttgga aagagtctcc caggtatctt
    14881 ccagcgcagc caacacaacc tcctgctgtg gtttcccagc aaacagatca accgcctgtg
    14941 ggattggtga tttgtctccc tgcaccgcct catacaaaat ttttgctcca acacttatat
    15001 tcagcgaacc agttgggccg tcctgggttg tttcgtagcc actggcgctg taccacttat
    15061 caaatggcat aggtacggca gccactacgg tacgcttcaa catactggtc agccaaacgt
    15121 tcaggatggc agagcctggc
//
LOCUS       DAZPEB010000096        15028 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZPEB010000096 DAZPEB010000000
VERSION     DAZPEB010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15028
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.097"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..452
                     /locus_tag="ACHURJ_002502"
     CDS             <1..452
                     /locus_tag="ACHURJ_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HFR1281403.1"
                     /translation="QEVNAALFKEYLPLLQQSEPTIKQPVRWKNALGELNANLDISIA
                     DPAKSSSSTNKDIKSLNFDVKLPLNVVTETAKQLNLSEGMDAEKAQKRADKQISGMMT
                     LGQMFQLITIDNNTASLQLRYTPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(493..1425)
                     /locus_tag="ACHURJ_002503"
     CDS             complement(493..1425)
                     /locus_tag="ACHURJ_002503"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFR1281404.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
     gene            1789..4575
                     /gene="polA"
                     /locus_tag="ACHURJ_002504"
     CDS             1789..4575
                     /gene="polA"
                     /locus_tag="ACHURJ_002504"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFR1281405.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLQQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNPLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRLHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            complement(4957..5589)
                     /gene="yihA"
                     /locus_tag="ACHURJ_002505"
     CDS             complement(4957..5589)
                     /gene="yihA"
                     /locus_tag="ACHURJ_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFR1281406.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRHSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            5886..6052
                     /locus_tag="ACHURJ_002506"
                     /pseudo
     CDS             5886..6052
                     /locus_tag="ACHURJ_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6171..6680
                     /gene="yihI"
                     /locus_tag="ACHURJ_002507"
     CDS             6171..6680
                     /gene="yihI"
                     /locus_tag="ACHURJ_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFR1281407.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            6869..8242
                     /gene="hemN"
                     /locus_tag="ACHURJ_002508"
     CDS             6869..8242
                     /gene="hemN"
                     /locus_tag="ACHURJ_002508"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFR1281408.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(8432..8542)
                     /locus_tag="ACHURJ_002509"
     CDS             complement(8432..8542)
                     /locus_tag="ACHURJ_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFR1281409.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(8653..10062)
                     /gene="glnG"
                     /locus_tag="ACHURJ_002510"
     CDS             complement(8653..10062)
                     /gene="glnG"
                     /locus_tag="ACHURJ_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFR1281410.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            complement(10074..11123)
                     /gene="glnL"
                     /locus_tag="ACHURJ_002511"
     CDS             complement(10074..11123)
                     /gene="glnL"
                     /locus_tag="ACHURJ_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFR1281411.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLEIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            complement(11297..12706)
                     /gene="glnA"
                     /locus_tag="ACHURJ_002512"
     CDS             complement(11297..12706)
                     /gene="glnA"
                     /locus_tag="ACHURJ_002512"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFR1281412.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            13079..14902
                     /gene="typA"
                     /locus_tag="ACHURJ_002513"
     CDS             13079..14902
                     /gene="typA"
                     /locus_tag="ACHURJ_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFR1281413.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDY"
     gene            complement(14954..>15028)
                     /locus_tag="ACHURJ_002514"
     CDS             complement(14954..>15028)
                     /locus_tag="ACHURJ_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1281414.1"
                     /translation="AFPRVVKERPWKYPTAPKKSQSVA"
BASE COUNT         3879 a         3770 c         3875 g         3504 t
ORIGIN      
        1 tgcaagaagt gaatgctgca ttgttcaaag agtatttacc gttattacaa caaagtgagc
       61 cgaccattaa acaaccggta agatggaaga acgcactcgg cgaactaaat gccaatctgg
      121 atatcagtat tgccgaccca gccaaatctt catcatccac aaacaaagat atcaaatcgc
      181 tcaattttga tgtgaagtta ccgcttaatg tcgtcacaga aaccgcaaaa cagcttaatt
      241 tatctgaagg aatggatgcg gaaaaagcgc aaaagcgggc tgataaacaa atcagcggga
      301 tgatgacctt aggtcagatg tttcagttaa tcacgattga caacaatacc gcctcgctgc
      361 aactgcgtta tacaccgggt aaagttgttt ttaacggaca ggagatgagc gaagaagaat
      421 ttatgtctcg tgccggacgt tttgttcatt aaaatactca ctcgcctctg ctcattcaga
      481 ggcgagcaaa atttacttgt cctgccgctg tgatgacatc aacgacgtca gaagtcggtc
      541 tttttcctgc cacagtgagt tcagccattg ctgaaaatgg cgcttaaagc ttttgtcatt
      601 gatgtagtcg ccatgtaact catcggcaat aggctgtaaa tccacatgaa cgacaattcg
      661 cgtcagttta ccgcttaaca tatcgaagaa cggctgacgg ttattgtccg gataacacag
      721 cgtaacatta agcagtttat cgaattgttt acccagtaca tttagcgcca tcgcaatgcc
      781 tgccgccttt ggtggcaaca agttttgaaa agtggagtgg gtttgctgat gtttttcttg
      841 cgtgaaacgg gagccttcaa cgaaattgac aatggtggtg ggatgcaggc gaaacttttc
      901 acaagaacgc cgagtggttt caacatcttt accacgtcgc tccggatgac gtaataaata
      961 agcgcgggaa taacgcttca taaacggcat atccagcgcc cagcacgcca ggccaagaaa
     1021 tggcacccag gccagctgtt gcttgaggaa atatttattc atcggaatgt gcttacgaaa
     1081 cagcacgcac agtacgacaa tatctgccca actacggtgg ttacaaataa gcagatacca
     1141 gttcttctta cttaaccctt ccagcccgtg aacttcccat tgcaggtgtg ggttcagatg
     1201 cagtaatacc gccagacctt cacaccagca atacatcata aaatcacaaa aacgcgaaac
     1261 ctttcgccaa atgactggca caggtagcaa tagttttaca atcccggcaa tgatgatcgg
     1321 gacggaacaa aaaatagtga ccaaaatggt caatacgata ctcagcaaaa gggttatcgc
     1381 agcgagtatt ctcgtcataa tgaatttatt caaaaggtta gccatagaca gtgcgctaaa
     1441 gaagcgcaag gcgcagattt tagcagaaaa cgacccaaat aacggatgat ccttaaggag
     1501 aaaaataatt catatctatc cacattagaa aaaatcccat tatctcaatt attagggatg
     1561 gctttatttt taactacatg aaaaacaaag acaaacatca tactgtaaaa agcatgataa
     1621 taaattaaaa gcgatgtaaa taatttatgc acaaagttat ccacatgacg atttgcgagc
     1681 gatccagaag atctacaaaa gattttcacg aaaagcggtg aaaaactcat gtttttatcc
     1741 tgtctgtggc atcctttacc cataatctga taaacaggca cggacattat ggttcagatc
     1801 ccccaaaatc cacttatcct tgtagatggt tcatcttatc tttatcgcgc atatcacgcg
     1861 tttcccccgc tgactaacag cgcaggcgag ccgaccggtg cgatgtatgg tgtcctcaac
     1921 atgctgcgca gtctgatcat gcaatataaa ccgacgcatg cagcggtggt ctttgacgcc
     1981 aagggaaaaa cctttcgtga tgaactgttt gaacattaca aatcacatcg cccgccaatg
     2041 ccggacgatc tgcgagcaca aatcgaacct ctgcacgcga tggttaaagc gatgggactg
     2101 ccgctgctgg cggtttctgg cgtagaagcg gacgacgtta tcggtactct ggcgcgcgaa
     2161 gccgaaaaag ccgggcgtcc ggtgctgatc agcactggcg ataaagatat ggcgcagctg
     2221 gtgactccaa atattacgct tatcaatacc atgacgaata ccatcctcgg accggaagag
     2281 gtggtgaata agtacggcgt gccgccagaa ctgatcatcg atttcctggc gctgatgggt
     2341 gactcctccg ataacatccc tggcgtaccg ggcgtcggtg aaaaaaccgc gcaggcattg
     2401 ctgcaaggtc tgggcgggct ggatacgctg tatgccgagc cagaaaaaat tgctgggttg
     2461 agcttccgtg gcgcgaaaac aatggcagcg aagctccagc aaaacaaaga agttgcttat
     2521 ctctcatacc agctggcgac gattaaaacc gacgttgaac tggagctgac ctgtgaacaa
     2581 ctggaagtgc agcaaccggc agcggaagag ttgttggggc tgttcaaaaa gtatgagttc
     2641 aaacgctgga ctgctgatgt cgaagcgggc aaatggttac aggccaaagg ggcaaaacca
     2701 gccgcgaagc cgcaggaaac cagtgttgca gacgaagcgc cagaagtgac ggcaacggtg
     2761 atttcttatg acaactacgt caccatcctt gatgaagaaa cactgaaagc gtggattgcg
     2821 aagctggaaa aagcgccggt atttgcattt gataccgaaa ccgacagcct tgataacatc
     2881 tctgctaacc tggtcgggct ttcttttgct atcgagccag gcgtagcggc atatattccg
     2941 gttgctcatg attatcttga tgcgcccgat caaatctctc gcgagcgtgc actcgagttg
     3001 ctaaaaccgc tgctggaaga tgaaaaggcg ctgaaggtcg ggcaaaacct gaaatacgat
     3061 cgcggtattc tggcgaatta cggcattgag ctgcgtggga ttgcgtttga taccatgctg
     3121 gagtcctaca ttctcaatag cgttgccggg cgtcacgata tggacagcct cgcggaacgt
     3181 tggttgaagc acaaaaccat cacttttgaa gagattgcgg gtaaaggcaa aaatccactg
     3241 acctttaacc agattgccct cgaagaggcc ggacgttacg ccgccgaaga tgctgatgtc
     3301 accttgcagt tgcatctgaa aatgtggccg gacctgcaaa aacacaaagg gccgttgaac
     3361 gtcttcgaga atatcgaaat gccgctggtg ccggtgcttt cacgcattga acgtaacggt
     3421 gtgaagatcg atccgaaagt gctgcacaat cattctgaag agctcacctt gcgtctggct
     3481 gagctggaaa agaaagcgca tgaaattgca ggtgaagaat ttaacctttc ttccaccaag
     3541 cagttgcaaa ccattctgtt tgaaaaacag ggtattaaac cgctgaagaa aacgccgggt
     3601 ggcgcgccgt caacgtcgga agaggtactg gaagaactgg cgctggacta tccgttgcca
     3661 aaagtgattc tggagtatcg tggtctggcg aagctgaaat cgacctacac cgacaagctg
     3721 ccgctgatga tcaacccgaa aaccgggcgt ttgcatacct cttatcacca ggcagtaact
     3781 gcaacgggac gtttatcgtc aaccgatcct aacctgcaaa acattccggt gcgtaacgaa
     3841 gaaggtcgtc gtatccgcca ggcgtttatt gcgccagagg attatgtgat tgtctcagcg
     3901 gactactcgc agattgaact gcgcattatg gcgcatcttt cgcgtgacaa aggcttgctg
     3961 accgcattcg cggaaggaaa agatatccac cgggcaacgg cggcagaagt gtttggtttg
     4021 ccactggaaa cagtcaccag cgagcaacgc cgtagcgcga aagcgatcaa ctttggtctg
     4081 atttatggca tgagtgcttt cggtctggcg cggcaattga acattccacg taaagaagcg
     4141 cagaagtaca tggaccttta cttcgaacgc taccctggcg tgctggagta tatggaacgc
     4201 acccgtgctc aggcgaaaga gcagggctac gttgaaacgc tggacggacg ccgtctgtat
     4261 ctgccggata tcaaatccag caatggtgct cgtcgtgcag cggctgaacg tgcagccatt
     4321 aacgcgccaa tgcagggaac cgccgccgac attatcaaac gggcgatgat tgccgttgat
     4381 gcgtggttac aggctgagca accgcgtgta cgtatgatca tgcaggtaca cgatgaactg
     4441 gtatttgaag ttcataaaga tgatgttgat gccgtcgcga agcagattca tcaactgatg
     4501 gaaaactgta cccgtctgga tgtgccgttg ctggtggaag tggggagtgg cgaaaactgg
     4561 gatcaggcgc actaagattc gcctgaacat gccctttttt cgtaagtaag caacataagc
     4621 tgtcacgttt tgtgatggct attagaaatt cctatgcaac aactgaaaaa aaattacaaa
     4681 aagtgctttc tgaactgaac aaaaaagagt aaagttagtc gcgtagggta cagaggtaag
     4741 atgttctatc tttcagacct tttacttcac gtaatcggat ttggctgaat attttagccg
     4801 ccccagtcag taatgactgg ggcgtttttt attgggcgaa agaaaagatc cgtaatgcct
     4861 gatgcgctat gtttatcagg ccaacggtag aattgtaatc tattgaattt acgggccgga
     4921 tacgccacat ccggcacaag cattaaggca agaaaattat tcgccgtcct gcgtttcttc
     4981 tacaggctgc atctcgctaa accaggtatc cagtttctgc cgcagcttgt ccacgccttg
     5041 tttcttcaac gaagaaaacg tttcaacctg cacatcaccg ttaaacgcca gtacagcttc
     5101 acgcaccata ttcaattgcg ctttacgtgc gccgcttgcc agtttgtccg ctttggtcag
     5161 cagcaccaga acggcgatat tgctgtctac cgcccactca atcatctgct gatccaaatc
     5221 tttcagcgga tggcgaatat ccattagcac caccagacct tgcaggctat gacgtttttc
     5281 gaggtattcg ccgagcgcac gctgccattt gcgcttcatc tcttccggga cttccgcata
     5341 accgtaccca ggcaagtcaa ccagacgctt gccgtcagcc acttcaaaca ggttgataag
     5401 ctgggtgcgc cctggggttt ttgaggtacg agccaggctt ttctggttag tcagcgtgtt
     5461 cagcgcgctg gatttacctg cgttggaacg gcctgcaaaa gccacttcaa ttccggtatc
     5521 ggaaggtagg tggcgaatat caggcgcact catcacaaaa tgcgtctgtt gataattcaa
     5581 attagtcaaa gcggtcgtct ccgtcagtca aagctttgtg gcgattatac ctgaaccata
     5641 ataaaaggcg gatttttcgg cgtgagcgtt gtaagtaaaa gccatacgct ttgtgagaca
     5701 ttgccgatag tctttatgcg aaatagcaga gaaaattctg cgatgcatgt caaataagct
     5761 atataaatca gtgaattgct tttatataaa actctgaaaa tgtgcaaata ctgatggcgg
     5821 ttgattgttt gtttaaagcg aaggcgtaaa gtagcaccca tagagcgagg acgctaacag
     5881 gaacaatgac tcaggatgag ggtcaggagc gccaggaggc gaagacagag gattgtcagg
     5941 aagacaaacg tccggagacg taattaaacg gaaatggaat caacacggat tgttccggct
     6001 aaaggaaaaa cagggtgtgt tggcggcctg caaggattgt aagacccgtt aagggttatg
     6061 agtcaggaaa aaaggcgaca gagtaatctg tcgccttttt tctttgcttg ctttctgtta
     6121 gattccgccg caaatcgata ctgaataaaa cggctaaaag acgaaccatt atgaaaccat
     6181 catcttcaaa ctcacgcagc aaaggtcatg caaaagcgcg tcgaaaaaca cgcgaggagc
     6241 tggatcagga agctcgtgat cgcaagcgtc agaaaaaacg tcgtggtcat gcgccgggca
     6301 gccgcgcagc gggcggaaac accacgtcag gcagcaaagg ccagaacgca ccaaaagatc
     6361 cacgtattgg cagtaaaacc cctattccat tgggcgtgac tgaaaaagtc accaaacagc
     6421 acaaaccgaa gagtgagaaa cctatgcttt caccgcaggc ggagttggaa ttactggaaa
     6481 cggatgagcg tctggatgcg ctgctggaac gtctggaagc aggcgaaacc ctgagtgccg
     6541 aagagcaatc ctgggtggat gctaaactgg atcgtattga tgagttgatg cagaaactcg
     6601 gcctctctta tgacgatgac gaagaagagg aagaagacga gaagcaagaa gacatgatgc
     6661 gtctgctgcg gggcaactaa cggattgccg ccgtgggcct tcccgttctg ctgataaccc
     6721 ttccggttat atgttatctg gtgtggttat tcgttaaact acagcggttg tcgcggcgac
     6781 aaaagtggct gcgcaaccgg ctgatgaccc gaaacgggca tccgccggta cgccgtagcc
     6841 gccagagacg ccatcggaag gagtgagcat gtctgtacag caaatcgact gggatctggc
     6901 cctgatccag aaatataact attccgggcc acgatacacc tcgtacccga ccgcgctgga
     6961 gttttcagaa gacttcggcg aacaggcgtt tttacaagcc gtggcgcgct atcctgagcg
     7021 tccattatct ctctacgtac atatcccgtt ctgccataag ctttgttact tctgcggttg
     7081 caataagatt gttactcgcc agcagcacaa ggccgatcag tatctggacg cgctggagca
     7141 agaaatcgtc catcgtgcac cgctgttcgc cgggcgtcac gtcagccaat tgcactgggg
     7201 cggcggaacg ccgacgtatc tgaataaagc gcaaatcagc cgcctgatga agctgctgcg
     7261 cgaaaacttc cagttcaatg ccgatgcgga gatttcgatc gaagtcgatc cgcgggaaat
     7321 cgaactggat gtactcgatc atttacgcgc cgaaggcttt aatcgcctga gcatgggcgt
     7381 gcaggacttc aacaaagaag tgcaacgtct ggttaaccgc gagcaggatg aagagttcat
     7441 ctttgcactg cttaaccatg cgcgtgagat tggttttacc tccaccaaca tcgacctgat
     7501 ttacggcctg ccgaaacaga cgccggagag tttcgccttt accctgaaac gtgtggcgga
     7561 gctgaacccc gatcgtctga gtgtctttaa ctacgcgcat ctgccgacca tttttgctgc
     7621 tcagcgcaaa atcaaagatg ctgacctgcc gagtccgcag caaaaactcg atatcctgca
     7681 ggaaaccatc gccttcctga cgcaatcggg ctatcagttt atcggtatgg atcactttgc
     7741 ccgtccggat gacgagctgg cggtggccca gcgtgaaggc gtgctgcatc gtaacttcca
     7801 aggctacacc actcagggcg ataccgatct gctggggatg ggcgtttccg ccatcagcat
     7861 gattggcgac tgctacgcgc agaaccagaa agagttgaag cagtactatc agcaagtgga
     7921 tgaacaaggc aatgcgctgt ggcgtggtat tgcgctaacg cgtgatgact gtattcgccg
     7981 cgatgtgatt aagtcgctca tctgcaactt ccgtctggat tacgccccta ttgagaaaca
     8041 gtgggatttg cacttcgctg attactttgc ggaagatctc aagctgctcg ccccgttagc
     8101 aaaagatggg ctggtggatg tggatgagaa gggaatacag gtgacggcga aaggtcgctt
     8161 gctgatccgc aacatttgca tgtgctttga tacctatctg cgccagaaag cgcggatgca
     8221 gcagttctca cgggtgattt gaatagtgaa tggcgcttcg tttacaaagt agaaaacgaa
     8281 agcgccatca atgtgctgag aacaagattg cctgatgcgc tacgcttatc aggcctacaa
     8341 aacctgttga aaatattgct tttgtaggcc ggataaggcg ttcacgccgc atccggcatg
     8401 aacaaagagc acttcatcag caatctgaaa ctcaactaaa cagcccaatc attgcggcac
     8461 acagcacggc agcaacggca gtttgcggtg tgtggctgtc aactgcgcca ctcgatacca
     8521 gattaattat tgattccagc ataatgactc tccccgtttt ccgggcagat catactgaac
     8581 ttatcggaac agtaaagcgt aaaataccag caatttgcgc tcaataatca atctttacac
     8641 acaagctgtg aatcactcca tccccagctc ttttaactta cgcgtcaggg tgttgcggcc
     8701 ccagccgagt agccgcgccg cttcctgttt atgcccctgc gtatgtcgca acgcggtcgt
     8761 cagtaacgtc cgctccagct ctggctgcgc ttcggaaagc agattttgat gaccggaacg
     8821 cagcgctctg tctgcccact gcgcaagaag cgtcgcccag ctgtccggtt gcatttgcga
     8881 agtactctcc gcaaccgttg attcaaacag ttcgccgggc aaatcctgaa tcaacacttc
     8941 ctgcccggcg gccatcaccg ttagccagcg gcaggtgttt tccagctggc gcacgttgcc
     9001 tggccacgcc agacgcgtca gagcagcttc ggtttccgga tgcagtaact tcgcttctac
     9061 gcccagttcg cgcgcggcaa cctgtaaaaa atggcgcgcc agacggggaa tatcttcccg
     9121 acgttcgcgc agcggcggta gatgaacgcg gataacgttt aggcggtgga acagatcctc
     9181 acggaactta ccttcctgca ctcgctgttc gagattctgg tgagtggcag cgataatccg
     9241 cacatccact ttcaccggcg catagccgcc aacgcggtaa aactgaccgt ctgccagcac
     9301 gcgcagcaaa cgcgtctgca catccagcgg catatcacca atttcgtcga ggaataatgt
     9361 accgccatcg gcctgttcaa aacgcccctg acgaatggta ttcgcgccag taaacgcgcc
     9421 tttctcgtgg ccaaacagtt ctgattcgat caaatctttt gggatagctg ccatattcag
     9481 cgcgataaac ggtgctttgg cgcgcggact gtggcgatgc agggcatgag cgaccagttc
     9541 tttaccggtg ccggattcgc cgttaatcag cacgctaata gaagaacgcg aaagccgacc
     9601 gataatacgg aacacgtcct gcatggctgg cgcttcgccg atgatatcgg tcgttgggcc
     9661 gttaagctga atattacgcg gctgctgctg ttcctggtaa tgactgatag cgcgctcaac
     9721 cagcgccacg gcttcgtcga tatcaaacgg tttgggcaga taatcaaacg ccccttgttg
     9781 ataggcgctg acggcagcat ccagatcgga atgtgcggtc ataatgatga ccggaagcat
     9841 tggatggcgc tgtttaatct gcttgagcag cgccagcccg tccattcccg gcatacggat
     9901 atctgaaagc agcacatccg gcgttttgct cgccagcgcc tccagtactt ccgcgccgtt
     9961 ctcaaatgtc gtacaggtta aacccgctcc agcgagcgca cgttcaagca cccaacggat
    10021 ggaactatcg tcatcgacta cccagactat ccctcgttgc ataaacgtca cctttatttc
    10081 ctgataggca ggtaaaccga gaactcggta tgacctggcc aactggtaaa ttcaattttg
    10141 cctgaatgct gatcaatcaa attacgggcg atggataagc caagcccggt gccaccttcg
    10201 cggccgctga ccatcgggta aaacagcgta tcctgcaaat gaggcggaat gcccggcccg
    10261 ttatcttcca catcaatccg cgccgccagc cggtagcgct cgccgtgtaa ggtcagttga
    10321 aacgcggtgc gggtacgcag aatgatttca ccgccttccg gccccagcgc ctgtagcgca
    10381 ttgcgcacaa tattcagcaa gacctgttca atttgatccg ggtcgtgcgc cagttccggc
    10441 aggctgggat cgtaatcacg aatcaaccgc acgttgtccg gcagttccat cgacaccagc
    10501 gttaccacgc gttcagccac tttgtgaata ctttcggtaa cgcgcgtacc gggcagctgc
    10561 ggccccaaca gacggtcgac cagatttcgc agccggtccg cctgttcgat aatcactttg
    10621 gtatattcga gtagtgatgg gtcaggtaac gctttgctga gcagctgcgc cgcgccacgt
    10681 aaaccgccaa gcggattttt aatctcatgt gccaggccgc gcactaaatc acgggcagca
    10741 acctgctggg cgtgctgtag ctgttcctga cttaagcggc gctggttatc catcggagcc
    10801 atctccagca ggatcatgcc gtccggcata cgctgggccg tcacagaaag gatatgcgag
    10861 cgcccgtcga tgaccagcgt cacttcgtta tcggtaaaac cttgccccgc ctccagactt
    10921 tcttgcatca gctcgatatt taatgagaag tagctcaaca gttccggtaa cggtgtacca
    10981 aacaatttgc gggagctttg ggcgagcagt tgttgcgcgg cagggttggc gtaatggatc
    11041 tccaggttgt catcgattaa caaaatactg ttaatcagcg agttgaggat ctgcccagca
    11101 tcgggctgcg tgcctgttgc cataaagcag tctcctgaac aggttgcacc attttagtgc
    11161 attatagctt tttacggata aaaagcgcga agcatcagag aattgacgga gaaaaaagcc
    11221 catgcagaga tgggctgaaa gtttccacgg caactaaatc ccggcgttgt tgcgccgggt
    11281 agtacaactt caactattag acgctgtagt acagctcaaa ctctaccgga tgcggagtca
    11341 tacgcacgcg gtcatcttct tcgcgacgca gagcgatgta cgcatcaatt gcttcgtcag
    11401 tgaacacgcc accggctttc aggaactcgc ggtccagatc cagttcgttc agtgcttctt
    11461 ccagagagcc tgcaacctgt gggatctctt tcgcttcttc tggcggcagg tcatacaggt
    11521 ttttgtccat ggcttcaccc ggatggatct tgttcttgat accatcaaga ccggccatca
    11581 gcagggcagc aaagcacagg tacgggttag ctgccggatc cgggaaacgt acttcgatac
    11641 gacgtgcttt cggagaagaa accaccggaa tacggataga cgcagaacgg ttacgcgcag
    11701 agtaagccag cattaccggt gcttcatagc ccgggaccag acgcttataa gagttggtgg
    11761 tcgggtttgc cagggcgtta atcgctttag cgtgtttgat tacgccgcca atgtagtaca
    11821 gcgcctgctc agacagacct gcgtatttgt cgcctgcgaa caggttaacg ccgtttttag
    11881 acagagacat gtggcagtgc ataccggagc cgttatcacc gaacatcggt tttggcataa
    11941 aggtcgcggt tttaccgaag cggtgcgcta cgttgtgcac aacatatttg tagatctgaa
    12001 tttcgtcagc ttttttggtc atggtattga agcgggtagc cacttcgttc tgaccagcag
    12061 tcgctacttc gtggtgatgg gcttcaacca ccagccccat ctgttccatc accagacaca
    12121 tttcagaacg aatatcctga gccgagtcta ccggtggaac cgggaagtaa ccgcctttca
    12181 ctgccggacg gtgacctttg ttaccacctt cgtattgggt ggaggagttc catgcgcctt
    12241 cgatatcgtc gatagcaacg tgggaaccgg agatagatga tccgaaacgg atgtcatcga
    12301 acaggaagaa ttcaggttct ggcccgaaca gtacggtgtc ggcaatgcca gtggaacgca
    12361 ggtaatcttc ggcgcgcttc gcaatggagc gcgggtcacg gtcatagcct tgcagggtgc
    12421 caggttcaag gatgtcgcaa cggataatca gggtggagtc ggcgaagaac gggtcaatca
    12481 ctgcggtgga tgcgtctggc atcagcacca tgtcggactc gttaatgcct ttccagccgc
    12541 caatcgagga gccgtcaaac attttgcctt cttcgaagaa ttcagcattc acctgatgag
    12601 cagggatagt gacgtgctgt tctttacctt tagtatcggt gaagcgcaaa tcaacaaact
    12661 tcacttcgtg ctcgttcagc atcgtcagta cgtgttcagc ggacatactt taactctcct
    12721 ggattggtca tggtcgtcgt ggtaacgaaa tctgcaatta ttttggccgt gtcgccgtaa
    12781 aaaagataaa gcgaaatctg tgccaacttt taaattgccc ctaaaaggcg ttatcatgcg
    12841 caccatcgtg caaaagggct gcaccacgat gtgaatgttg caccaatata gtgcttcaat
    12901 ggaaacatta agcaccatgt tggtgcaatg acctttggat aacccttttt atgctccgtg
    12961 aaagcgatca caaagggact ctgcaatact tgtttgcgga ggatgtttgt gatcctgttt
    13021 tgtagtgcga ttaatccgtg tacaataacg cgctatttct aatgcctgag gcaaagttgt
    13081 gatcgaaaaa ttgcgtaata tcgccatcat cgcgcacgta gaccatggta aaaccaccct
    13141 ggtagacaag ctgctccaac aatccggtac gttcgactct cgtgccgaaa cccaagagcg
    13201 cgtgatggac tccaacgatt tggagaaaga gcgtgggatt accatcctcg cgaaaaacac
    13261 cgctatcaaa tggaatgatt accgtatcaa catcgttgat accccggggc acgccgactt
    13321 cggtggtgaa gttgaacgtg taatgtccat ggtagactca gtgctgctgg tggttgacgc
    13381 atttgacggc ccgatgccgc aaacgcgctt cgtaaccaaa aaagcgtttg cttacggcct
    13441 gaagccgatt gttgttatca acaaagttga ccgccctggc gcgcgtcctg attgggttgt
    13501 ggatcaggta ttcgatctgt tcgttaacct cgacgcgacc gacgagcagc tggacttccc
    13561 gatcgtttac gcttctgcgc tgaacggtat cgcgggtctg gaccacgaag atatggcgga
    13621 agacatgacc ccgctgtacc aggcgattgt tgaccacgtt cctgcgccgg acgttgacct
    13681 tgacggtccg ttccagatgc agatttctca gctcgattac aacagctatg ttggcgttat
    13741 cggcattggc cgcatcaagc gcggtaaagt gaagccgaac cagcaggtca ctatcatcga
    13801 tagcgaaggc aaaacccgca acgcgaaagt cggtaaagtg ctgggccacc tcggtctgga
    13861 acgtatcgaa accgacctgg cggaagctgg cgatatcgtt gcgatcacgg gccttggcga
    13921 actgaacatt tctgacaccg tttgcgacac gcaaaacgtt gaagcgctgc cggcactctc
    13981 cgttgatgag ccgaccgttt ctatgttctt ctgcgttaac acctcgccgt tctgcggtaa
    14041 agaaggtaag ttcgtaacgt ctcgtcagat cctggatcgt ctgaacaaag aactggtaca
    14101 caacgttgcg ctgcgcgtag aagaaaccga agacgctgat gcgttccgcg tttctggtcg
    14161 tggcgaacta cacctgtctg ttctgatcga aaacatgcgt cgtgaaggtt tcgaactggc
    14221 ggtatcccgt ccgaaagtta tcttccgtga aatcgacggt cgtaaacaag agccatatga
    14281 aaacgtgact ctggacgttg aagaacagca tcagggttct gtaatgcagg cgctgggcga
    14341 acgtaaaggc gacctgaaaa acatgaatcc agacggtaaa ggccgcgtac gtctcgacta
    14401 cgtgatccca agccgtggtc tgattggctt ccgttctgag ttcatgacca tgacttccgg
    14461 tactggtctg ctgtactcca ccttcagcca ctacgacgac gtacgtccgg gtgaagtggg
    14521 tcagcgtcag aacggcgtac tgatctctaa cggtcagggt aaagcggtcg cgttcgcgct
    14581 gttcggtctg caggatcgcg gtaagctgtt cctcggtcac ggtgcagaag tttacgaagg
    14641 tcagattatc ggtattcata gccgctctaa cgacctgact gtaaactgcc tgaccggtaa
    14701 gaaactgacc aacatgcgtg cttccggtac tgacgaagcc gttgttctgg ttccgcctat
    14761 ccgcatgact ctggaacaag ctctggagtt catcgatgat gacgaactgg tagaagtgac
    14821 tccgacctct atccgtattc gtaaacgtca cctgacggaa aacgatcgtc gtcgcgccaa
    14881 ccgcgcaccg aaagactatt aatttcgttt tttagttata aaaaacctgc catcgctggc
    14941 taatgccagt cagttaagca actgactggc tctttttcgg ggctgtgggg tatttccagg
    15001 gcctctcctt taccactctc gggaaggc
//
LOCUS       DAZPEB010000097        14833 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZPEB010000097 DAZPEB010000000
VERSION     DAZPEB010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14833
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.098"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..244
                     /locus_tag="ACHURJ_002515"
     CDS             <1..244
                     /locus_tag="ACHURJ_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFR1281415.1"
                     /translation="DEWKPKRFSLPITNTTLVKYGKLGYIVPRPQKIRGRWLIDRRAV
                     FVGPGETGIAPEIHTGDDDALKEILTHVTEATKKQH"
     gene            213..1319
                     /locus_tag="ACHURJ_002516"
                     /pseudo
     CDS             213..1319
                     /locus_tag="ACHURJ_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074982.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
     gene            1499..2293
                     /gene="potC"
                     /locus_tag="ACHURJ_002517"
     CDS             1499..2293
                     /gene="potC"
                     /locus_tag="ACHURJ_002517"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFR1281416.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMISPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            2290..3336
                     /gene="potD"
                     /locus_tag="ACHURJ_002518"
     CDS             2290..3336
                     /gene="potD"
                     /locus_tag="ACHURJ_002518"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFR1281417.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETCIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(3492..4313)
                     /gene="cobB"
                     /locus_tag="ACHURJ_002519"
     CDS             complement(3492..4313)
                     /gene="cobB"
                     /locus_tag="ACHURJ_002519"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFR1281418.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     KIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(4329..5240)
                     /gene="nagK"
                     /locus_tag="ACHURJ_002520"
     CDS             complement(4329..5240)
                     /gene="nagK"
                     /locus_tag="ACHURJ_002520"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFR1281419.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCRGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQQLQAPEIIALYNQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(5269..6513)
                     /gene="lolE"
                     /locus_tag="ACHURJ_002521"
     CDS             complement(5269..6513)
                     /gene="lolE"
                     /locus_tag="ACHURJ_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFR1281420.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDKVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEMTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     LIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(6513..7214)
                     /gene="lolD"
                     /locus_tag="ACHURJ_002522"
     CDS             complement(6513..7214)
                     /gene="lolD"
                     /locus_tag="ACHURJ_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFR1281421.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(7207..8406)
                     /gene="lolC"
                     /locus_tag="ACHURJ_002523"
     CDS             complement(7207..8406)
                     /gene="lolC"
                     /locus_tag="ACHURJ_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFR1281422.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSINPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRMMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGNEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEDSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMIFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPMAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            8668..9741
                     /locus_tag="ACHURJ_002524"
     CDS             8668..9741
                     /locus_tag="ACHURJ_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFR1281423.1"
                     /translation="MKQKELWINQIKGLCICPVVIYHSVITFYPHMTTFPHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            9869..13315
                     /gene="mfd"
                     /locus_tag="ACHURJ_002525"
     CDS             9869..13315
                     /gene="mfd"
                     /locus_tag="ACHURJ_002525"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFR1281424.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTKHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDVARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            13459..14421
                     /gene="ldtC"
                     /locus_tag="ACHURJ_002526"
     CDS             13459..14421
                     /gene="ldtC"
                     /locus_tag="ACHURJ_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFR1281425.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     TRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(14504..14761)
                     /gene="bhsA"
                     /locus_tag="ACHURJ_002527"
                     /gene_synonym="ycfR"
     CDS             complement(14504..14761)
                     /gene="bhsA"
                     /locus_tag="ACHURJ_002527"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFR1281426.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISAKAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         3719 a         3858 c         3769 g         3487 t
ORIGIN      
        1 cgacgagtgg aaaccaaaac ggttcagtct cccaataaca aacactaccc tggtgaaata
       61 cggaaaacta ggatacatcg ttccaagacc acaaaaaatt cgtgggcgtt ggctgataga
      121 tcgccgagca gtatttgttg ggccaggtga aacgggaatt gcgccggaaa ttcatactgg
      181 cgatgatgat gcactgaagg agattttaac tcatgtcacc gaggccacga aaaaacagca
      241 ctgacgtagc cggtctttac gaaaagtttg atcgcagaac tggcagagtt tactaccagt
      301 ataaaaatcc tgtgactgga aaatttcacg gactcggaac agacaaaggt aaggcagaaa
      361 aaatcgcttc cacagccaat cagcgaatag ctgcagcaga agctgaatat ttcatgcgca
      421 aaattgatga aagtccgtca gcaacaaaac gtcggggtat cagattaaag gcatgggttg
      481 atcgatatct gaaaatacag gacacgcgac tgaaaaatgg agatattgca gctacaactc
      541 acaaagaaaa aactcgaatg gctgcatacc tggtttcccg tctgggaaac cacccattga
      601 aagaactgga agtaagagac tttgcattaa tactggatga gtggctggat aaagacatgg
      661 tcagcacagc gagagtaaat cgtggattat gggttgatat ttataaagaa gcacagcatg
      721 caggggaagt tcctcctgga tggaatcctc cggaggctac ccgtaaaccg atccctaaag
      781 taaccagagc caggctcacc atggaagact ggcaaaaaat ttacaatgca acgcctgaaa
      841 aacactttat ccgtaacgca atgcttcttg cgattgttac tggtcagcgc cgtgatgaca
      901 tttgccacat gcgtttttca gatgtgtgga acgaacactt gcatatcacc tagggaaaaa
      961 ccggaatgcg tctggcgtta ccgcttacac tacgctgtga tgccattggg ataacgttaa
     1021 aagaagttat tgatgggtgc cgagacagaa tattaagtcc atatctaatc catagtcggc
     1081 accagaaaca accgaagccg atgagtaaag acaacctgag cgactacttt gccaaagcac
     1141 gggatctggc tgggataatt ccaccagcag gaaaaactcc gccaacattt catgaacaac
     1201 gctctctatc agaacggctg taccgtgcac agggtatcga tacaaaaaca ttactaggac
     1261 ataaagtcca ggcaaccacc gatcgctata acgatactcg aggtcaggaa tgggtttaag
     1321 ctggttattt gacaaacacc atatggaata tccgtcacca tctcaggaaa aaagtgttgt
     1381 aaaatgcgtg gcctagtttt ggggaaacgt tttggagaga ttttggagaa aggaaaaaat
     1441 tgaataaatt caaaatcctg aaagtgctcc accttatgaa taagaaggtg gaactcgaat
     1501 gatcggtcga ctgcttcgcg gcggttttat gaccgctatc tacgcgtacc tgtatatccc
     1561 aatcattatt ttgattgtga actcctttaa cagctcgcgc tttggcatca actggcaggg
     1621 ttttaccacc aaatggtata gcctgctgat gaacaacgac agcctgttac aggcagcgca
     1681 gcattcacta acaatggcgg tgttttcggc gacgtttgct acgcttatcg gttcactgac
     1741 ggcagttgcg ctgtaccgtt atcgctttcg cggtaagccg ttcgttagcg gaatgctgtt
     1801 tgtggtaatg atatcaccag atatcgtgat ggcaatttcg ctgctggtgc tgtttatgct
     1861 gctgggtatt cagcttggct tctggtcgct gctgttctcg catatcacct tctgcctgcc
     1921 atttgtggtg gtgacggtgt attcgcgcct gaaaggtttt gacgtgcgga tgctggaagc
     1981 ggcgaaagat ctcggtgcca gcgaatttac aattctgcga aaaatcatac tgccactggc
     2041 aatgccagcg gtggcagcgg gctgggtgtt aagctttacc ctgtcgatgg acgatgtggt
     2101 agtttcttcg ttcgtcaccg gaccaagtta tgaaattctg ccgttaaaaa tttattcgat
     2161 ggtcaaagtc ggcgtatcgc cggaagttaa cgcgctggca accatattac tggtgctgtc
     2221 gctggtgatg gttattgcca gtcagcttat tgctcgtgat aaaacgaaag gtaacacagg
     2281 ggacgttaaa tgaaaaaatg gtcacgccac ctgctcgcgg cgggtgctct ggcactgggc
     2341 atgagcgccg ctcacgccga tgacaacaac acgctgtatt tctacaactg gaccgagtac
     2401 gtgccgccag gactgcttga acagttcacc aaagaaacct gtattaaggt tatctattcg
     2461 acttacgagt cgaacgaaac catgtacgcc aagctgaaaa cgtacaaaga cggtgcgtat
     2521 gacctggtgg ttccttcaac ctattacgtc gataaaatgc gtaaagaagg gatgatccag
     2581 aagatcgaca agtcgaagtt aacgaacttc agcaatctcg atccagacat gctcaacaag
     2641 ccttttgacc cgaataacga ctactccatt ccgtatatct ggggggcgac ggcgattggt
     2701 gttaacggtg atgcggtgga tccgaaatct gtcaccagct gggccgatct gtggaagcca
     2761 gagtacaaag gcagcctgct gttgaccgac gatgcccgtg aagtgttcca gatggcgctg
     2821 cgtaagctgg gctattccgg taacaccacc gatccgaaag agattgaagc tgcatataac
     2881 gagctgaaaa aactgatgcc aaacgtcgca gcgtttaact ccgataaccc ggctaacccg
     2941 tacatggaag gcgaagttaa cctcggcatg atctggaacg gttctgcttt cgttgcacgc
     3001 caggcgggta cgcctattga cgtggtgtgg ccgaaagaag gcggcatatt ctggatggac
     3061 agcctggcga tcccggcaaa tgccaaaaac aaagaaggcg cgctgaaatt gatcaacttc
     3121 ctgctgcgcc cggatgtggc aaaacaggtt gctgaaacta tcggttatcc aacgccaaac
     3181 cttgcggcgc gtaagctgtt aagtccagaa gtggcgaacg ataaaacgct ctacccggat
     3241 gctgaaacca ttaaaaatgg cgaatggcag aatgacgttg gcgcagccag cagcatttat
     3301 gaagagtatt atcagaagct gaaagcagga cgttaattca gcaagcgggc ggtggtatta
     3361 ccgcccgttt tttatttagc aaggatgtgg ctaatgagtc aggacagtag agttttatcc
     3421 gactctttcg tcggtattgt ggtccgctcg taggcctgat aagcgtagcg catcaggcaa
     3481 tgcttcctgc tttataatcc cttcagcaac ttttcaacaa actcaggcac cacctggctt
     3541 gccgggccgt aatatttctc ggcaaattca ttaccgacct gacttggttc gagattcagt
     3601 tccacggtgt gcgcgccatg cagtttcgct tcgtgaacaa acccagccgc cggataaaca
     3661 tgcccggaag taccaatggc aatgaaaata tcagccatcg acaacgccat ataaatttca
     3721 tccatgccga gtggcatttc gccaaaccaa acgacgtgcg ggcgcaaggg tgccgggaac
     3781 tggcaacaat ggcatttatc ttctggcgta acgtctccgg tccagtcgag aacctgacca
     3841 ctttgcgaac aacgaacttt cagcaattcc ccatgcatat gaatgacatt ggtattacct
     3901 gcgcgttcat gcaggttgtc tatattctgc gttaccagca aaaagcgatc gccgagagcg
     3961 tcttgcagtt tagccagcgc aagatgcgcg gcgttaggct gaatttttgg ctgctgcagc
     4021 tgtcgacgac gggcgttata aaacgcttgc accagttcag gatcgcgatc gaaaccttcc
     4081 ggcgttgcca catcttcaac ccgatgttct tcccacaggc catctgcggc gcgaaaggta
     4141 cgaatacctg attccgcaga aattcctgcc cctgtcagta cgagtactct tggtttttcc
     4201 attgcttccg gcaccacttt atctctgaaa aaaatacgct gacgcaaacg ctcgcgcagg
     4261 cggcgtttat ttttacgaaa acgacttaac cgatgacccc gacgcgacag catagcaacc
     4321 tctgttgttt aatcggttag atgtaggaag gccgcaccac gcattcctcc cgcatcaccg
     4381 tggcgcgcgc gttcaatgcg cggaacacga gctacaggta agagatgacg aggcagcctg
     4441 tccgccagtt gcgttgtgat tgccgggaaa ttcgataagc caccaccaat gacgaccagg
     4501 tcagggtcaa caatggtcag gatatttccc agacaaaccg ctaataaatc cagataacgc
     4561 tcaacgtgcg cccttgcctg ctcatcgcct tgattataaa gcgcaataat ttcgggagcc
     4621 tgcaactgtt gatgataata gtgttgatac agccacgcaa aaccgcgacc agacagataa
     4681 ttctcaatgc agccatgctg accacagccg cagcggcgta acgggaaatc caaccccatc
     4741 atggttaacg catcaaccgg cagacgcata tggccaaact cgccggtaat gtagctttta
     4801 ccggtaatcg gtttgccgtt gaaaatcagc ccaccgccaa cgccggtacc aagaatcaac
     4861 cccatcacca gtggatattg cgtaaattca tcatcccagg cttctgaaag ggcaaaacag
     4921 ttggcatcgt tatcaaggcg tacatcgcga tcaagacgtg cgctcaggtc ggcacgcagc
     4981 ggtttaccgc tggcagcggg gacattggcg gcatacagcg taccatcttc tgtttccggc
     5041 attcccggta taccaatgcc gacagaacct ctacagccaa aacgttgatc agcttcggct
     5101 accagctcgc acactgcatc taaaaatgcg tcatagctgt cacgcggtgt cggcacccgc
     5161 ttttcccact gcaactgccg accgctatca aacacgccaa gcgcaatttt tgttccacca
     5221 atatcaaacc cgtaatacat tgccgctcct tgttttaatg tactgccttt actggccgct
     5281 aaggactcgc gcagggtcaa tattactggc gcgccgcgcc ggataccaac ttgccaaaag
     5341 actcagcaac aatgctgtga ccagtacgta gaagacgtcc agccagtgca attccgatgg
     5401 caggaagtca ataaaataga tatcgctgga gaggaactga tgaccgatca gcttttcaat
     5461 ccgctcaata atcggggtaa gttgcagtga aacaactacg ccgataatca gcccacacag
     5521 gctgccgaac agccctgcca gcaatccata ccagacaaag atggcgcgaa ttaaaccatc
     5581 tttcgccccc agcgttctta atactgcgat atcgccactc ttatctttca ccgccatcac
     5641 taaggtggag acgatgttga aacaggccac gccaatcacc agtaccatcg ccagatacat
     5701 aatggcgcgg atcatctgga tatcgcgata catatagccg taagtaccaa tccagctttt
     5761 aatataaaca tagctgttgg tcatttcacc cgcatcgcgt accagcttat tggcgttgaa
     5821 aacatccgtc attttaaggg caatacctga cacgctggaa cccatatcaa gatattgttg
     5881 ggcatccgcc agcgggatca tggcaaaact gtgatcgagt tggccactca actgcaaaat
     5941 acccgcaacg tgcaaacgca cacgttttgg ctgcatcagt ttatgctcag ggttcgagtt
     6001 ggggatcatg atcgacaccc aatcgccctg cttcactttc agcgcatccg ccacgccttt
     6061 gccgatgata atttgctgtt cgcccgcttt aaaattgcgc cacgcatccc cctgaacaaa
     6121 cgagggtaat gcgctcagac gctgttcctg ttgtgggtta acgcccttca cctggattgc
     6181 gcgcagattc gctccacttt ccaccagccc ggtgaaattg atatacggcg cagcggcggc
     6241 aatacccggc actttctgca ctttatccag tgcttcctgc cagttagtcc atggctgatt
     6301 taccgcttcg atttcaccat gcggcaccac cgccagaatg cggttattca gttcgcgttc
     6361 aaagccgttc atcgcgctta agccgacgat caataccgcc acaccgaggg caatgccaat
     6421 ggtagaaatg acggagatca gcgacaccat gccgccgcgc cgccgtccgc gactaaaacg
     6481 caggccaatt aataacgata aaggcatcgc cattactccg cccccatcag gctcagttcc
     6541 gccgtcagac gtccatcacg catttctaac tggcggctca tacgtttcgc cagttgcagg
     6601 tcgtgagtaa ccaccaggaa ggcggtgccc tgcaagcgat tcaattcccc aagcaactgg
     6661 aagatgctgt cggcgttacg cgcatcgagg ttaccggtag gctcatccgc cagtaccaga
     6721 cgcggattat tgaccagcgc acgggcaatc gctacacgct ggcgttcgcc gccagaaagt
     6781 tcagatgggc ggtgattcgc acgatgctcc agccccaccg cttttaacat ctccagtgca
     6841 cggctgttga tttcagcggg ctttttcttg ccaatcagca gcggcatagc cacgttttcc
     6901 agggcggtaa aatccggcag caggtggtga aactgataaa taaagcccag tttctggttg
     6961 cgcagttctg cttttgctgc cgacgacagt ttgctcatcg gttgaccgtt aaagatcaca
     7021 tcgccggagg ttggcgtatc cagtccgccc agcaggtgca gcaaggtgct tttaccggaa
     7081 ccagagctac cgacgatcgc catcatttca ccttcgccga cgctgaaact gacattgtgc
     7141 agtacatcgg tttgcacact gccttcctga tagcgtttgc acaggttgtc gcattgcaac
     7201 aggatcttat tcataacgta aagcctcagc gggttgtgtg gcggcagcgc gccatgaagg
     7261 gtaaagcgta gacagcagcg cgatagccat cgccaccagc gcaataacaa tgacctgtaa
     7321 aggttcgata gccatcggca gcgccgcgcc atcaagcagg acgccgatta ttggcatcag
     7381 attatttaac tggctggcaa gcagtgcgcc aagcgccgct ccgaggatcg caccgataat
     7441 cccggcgctg gccccttgca ccataaagat catcatgatt tgtcgcggag ttaagccttg
     7501 cgtttgcagg atcgctactt cgccctgctt ctccatcacc atcagcccca gcgaggtaat
     7561 aatgttaaac gccgcaacgg cgacaatcag gctcagcagt aaacccatca tatttttttc
     7621 catgcgtacg gcctggaaca actcgccttt acgatcacgc cagtcctgcc atttgctgtc
     7681 ttcaggcagt ttttgctgac ttaatgagtc gactttcagc ggctcatcca gccacaaacg
     7741 ccagccggta atattgcctg ccggataacg catcagacgc gaggcatcct caatattcac
     7801 cagcatttca ttgccatcga cttcactgtt ggcggcgaaa gtgccaatca cattgaacag
     7861 gcgctgactt ggaatacgcc ccatcggcgt gaactggctg gcagatggta ccatcatgcg
     7921 gatttgatca ccgcgattaa cgcctagctg tgaggcaagt tgttcgccga ggatgacatt
     7981 atatttcccc ggctcgaggt cagtttgttt cacattgacc agatacggcg ttagtggatc
     8041 tttttgcgcc ggatcgatac ccagcatcac cccgaccgcc acgctgcgcg cgctttgcag
     8101 taccacatca ccggtagtaa taggtgcgac gcgattaacg ccgtccagtt tgactgccgt
     8161 ttccgggagt tgctgcgggt taatagagcc atgctcagaa gagagaattg cctgtggcat
     8221 caggccaagg atgttgtttt gcagctcgcg ctcaaagccg ttcatcactg acaatactgt
     8281 gaccagcgcc atcaccccga gggtaatgcc gatggtagaa agccaggaga cgaaacgacc
     8341 gaagcgatcc gctgcacgcc cacgcatgta acgcaggcca ataaatagag cgacaggttg
     8401 gtacatgaaa tccgtctggt tgctgtagca aagacacgag tatataagcg aaagcaagat
     8461 gggtaaacgt ctgaactgta agtgatcgtc agggctaatc tgaaaagccc ccgtaaatca
     8521 atcggctaaa ttgccatgtt ttatcatcaa tcttgccgcc tgatcttaat ctgaaaaatc
     8581 atccaataca gacagatacc cattacacct cgggtcataa cccgcaggat caaccgcatg
     8641 taaattaaaa aagagcgtgt tgtcctgatg aaacaaaaag agctatggat taaccagatc
     8701 aaagggttat gtatttgtcc ggtggtgatt tatcactcgg tcattacctt ttatccgcat
     8761 atgaccactt ttccgcatcc gttatcggaa gtcctgagca aatgctggat ctatttcaat
     8821 ctttaccttg caccctttcg tatgccggtt tttttcttta tatccggcta tttgattcgc
     8881 cgctatatcg acagcgtacc gtggggaaat tgtctcgata aacggatctg gaacatcttc
     8941 tgggtgctgg cgctttgggg cgtggtgcag tggctggcgt taagtgcgct gaaccagtgg
     9001 ctggcacctg agcgcgattt aagcaatgcc tccaatgccg cttatgccga ttccaccggt
     9061 gagttcctgc acgggatgat caccgccagc accagcttgt ggtatctgta tgcgttaatt
     9121 gtctatttcg tggtatgtaa aatttttagc cgtctggcgc tgccactatt cgccttgttt
     9181 gtactgctga gtgtggcggt taatttcgtt cccacgccgt ggtggggaat gaacagtgtg
     9241 atccgcaatt tgccttatta cagccttggc gcatggtttg gcgcaacaat aatgacctgt
     9301 gttaaagagg taccgttgcg ccgccatctg ctgatggctt ctttgcttac cgttctggcg
     9361 gtcggtgcct ggttgtttac tatctcgctg ctgttgtcgc tggtatcgat tgtggtgatc
     9421 atgaagctgt tttatcagta cgagcaacga ttcggtatgc gctccaccag cctgctgaat
     9481 gtgattggct ccaacaccat tgctatctac accacccatc gcattctggt tgaaatattc
     9541 agcttaactc tgcttgcgca aatgaacgca gcacgctggt cgccgcaagt cgaactgaca
     9601 ctcctgctgg tttatccctt tgtcagtttg ttcatctgta ctgttgcggg cttgctggta
     9661 agaaaacttt cacagcgtgc attcagcgat ctgttgttct ccccgccttc actgcccgct
     9721 gccgtcagtt actcccgcta agccatcgcc ccagccgggg cgattcaatt tgctaaacca
     9781 tgtcgattac ggataatgaa cagcattgct tatcagagat atgcccccat atgttgaggc
     9841 atatcctaac gagaatctga caaccgttat gcctgaacaa tatcgttata cgctgcccgt
     9901 caaagcgggt gagcagcgtc tgctgggcga gttaaccggc gcagcctgtg caacgctggt
     9961 agcggaaatt gccgaacgtc acgccggtcc ggtggtactc attgcaccag atatgcaaaa
    10021 tgctctgcgt ttgcatgatg aaatcagtca gttcaccgat cagatggtga tgaatctggc
    10081 ggactgggaa actcttccct acgacagctt ttcgcctcat caggacatta tctcctcgcg
    10141 cctttccacc ctttaccagc taccgacgat gcagcgtggc gtactgattg ttccggtgaa
    10201 tacgcttatg cagcgcgttt gcccacacag ttttctccac ggtcatgcgc tggtgatgaa
    10261 aaaaggtcag cgcctgtcac gagatgcatt acgaacccaa ctggacagcg ccggttatcg
    10321 ccatgttgac caggtgatgg agcacggcga atacgccacg cgcggcgcgt tgctggatct
    10381 cttcccgatg gggagtgagc tgccttatcg tcttgatttc tttgatgatg aaatcgacag
    10441 cctgcgggtg tttgacgtcg acagccagcg cacgctggag gaagtagaag cgatcaatct
    10501 gctgcccgcg cacgaatttc cgaccgataa agcggcaatt gaactgttcc gcagccagtg
    10561 gcgcgatacc ttcgaagtga agcgcgatcc ggaacatatt taccagcaag tgagtaaagg
    10621 cacattacct gccgggatcg agtactggca gccattgttc ttcagcgaac cactgccgcc
    10681 gctgttcagt tatttccctg ccaatacctt gctcgtgaat actggcgatc tggaaaccag
    10741 tgccgaacgt ttccaggctg acacgctggc gcgttttgag aatcgcggcg tcgatccgat
    10801 gcgcccgctg ttgccaccac aatcgctctg gctgcgggtg gacgagctct tctcagagct
    10861 gaaaaactgg ccccgggtgc agctaaaaac taaacattta ccgacaaaag ccgcgaatgc
    10921 caatttaggt ttccagaaac tgccagacct agccgttcag gcgcaacaaa aagcgccgct
    10981 ggatgcgctg cgtaagttcc tcgagacttt cgacggtccg gtggtgttct cggtagaaag
    11041 tgaaggtcgc cgtgaagcgc tgggtgaact gctcgcacga attaaaattg ctccgcaacg
    11101 cattatgcgt cttgatgaag ccagcgaccg tgggcgttat ctgatgattg gcgctgccga
    11161 acatggtttt gtcgataagg tgcgtaatct ggcgctgatc tgcgaaagcg atctgctcgg
    11221 tgaacgtgtt gcccgccgtc gtcaggattc tcgccgcacc atcaaccccg atacactgat
    11281 ccgtaacctt gcggagctgc atattggtca gccggtggtc catctggagc acggtgtcgg
    11341 gcgctatgcc ggaatgacca cgctcgaagc gggcggcatt actggcgagt atttgatgct
    11401 cacctatgcc aacgacgcca aactgtatgt tccggtgtcg tcactgcatc tgattagccg
    11461 ttacgcaggt ggcgcggaag aaaacgcccc gctgcataaa cttggcggcg atgcgtggtc
    11521 acgcgcgcgg cagaaagcgg cggaaaaagt gcgtgatgtg gcggcggaat tgctggatat
    11581 ctacgcgcaa cgcgccgcca aagagggctt cgcgtttaaa cacgatcgtg agcagtatca
    11641 gttgttctgc gacagcttcc cgtttgaaac cacgccggat caggcgcagg ccattaatgc
    11701 ggtacttagc gacatgtgtc agccgctggc aatggatcgt ctggtgtgcg gcgatgttgg
    11761 ctttggtaaa acagaagtgg cgatgcgcgc cgctttcctg gcagtagata accacaagca
    11821 ggtggcggtg ttggtgccta ccacccttct cgcgcagcag cattacgaca acttccgcga
    11881 ccgtttcgcc aactggccgg tacgtatcga aatgatctcc cgtttccgca gcgccaaaga
    11941 gcagacgcaa atccttgcgg aagtggcgga agggaaaatc gatattctga tcggtacgca
    12001 caaactgctg caaagtgacg tcaagtttaa agatttaggc ctgctgattg tcgatgaaga
    12061 acaccgcttc ggggtgcgtc ataaagagcg cattaaagcg atgcgcgcga acgtggatat
    12121 tctgacgctt actgcaacgc cgatcccacg cacgctgaat atggcaatga gcggaatgcg
    12181 tgacctgtcg attatcgcca cgccgcccgc ccgtcgtctg gcagttaaaa cctttgtccg
    12241 tgagtatgac agcctggtgg tccgggaggc gatcctgcgt gaaattttgc gcggggggca
    12301 ggtttattat ctctacaatg atgtggaaaa catccagaaa gctgccgaac ggctggcaga
    12361 actggtgcca gaagcgcgga tcgccatagg tcacgggcag atgcgcgagc gcgaactgga
    12421 acgggtgatg aatgatttcc atcatcaacg tttcaacgtg ctggtttgta caaccattat
    12481 cgaaaccggg atcgacatcc cgacagccaa caccattatc attgaacgcg cggatcactt
    12541 cggtctggcg cagctgcacc agttacgcgg tcgcgtcgga cgttcacatc atcaggcata
    12601 tgcatggctg ctgacgccgc atccaaaagc gatgactacc gatgcacaaa aacgtcttga
    12661 agcgattgcc tcgctggaag atctcggtgc aggctttgcg ctggcaacgc acgatctgga
    12721 gatccgcggc gcgggtgaac tgcttggcga agaacaaagt ggctcaatgg aaaccatcgg
    12781 tttctcgctg tatatggagt tgctggaaaa cgccgtcgat gcactgaaag ccggacgcga
    12841 gccgtcgctg gaagatctca ccagccagca aacagaagtc gagctgcgga tgccgtcgct
    12901 attgccagat gatttcattc ctgacgtgaa tacgcgtttg tcgttctata aacgtattgc
    12961 cagcgccaaa acggaaaacg aactggaaga gatcaaagtc gagcttatcg atcgcttcgg
    13021 cctgctgccg gatccggcgc gtaccctgct ggatgttgcc cgtctgcgcc agcaagcgca
    13081 gaaactgggg atcaggaagc tggaaggtaa tgagaaaggc ggggtgatcg aatttgccga
    13141 gaagaatcac gttaatccgg cctggttgat tggtttgctg caaaaacagc cgcagcatta
    13201 ccgccttgat ggtccgacgc gcctgaaatt tattcaggat ttgagtgagc ggaaaacgcg
    13261 tatcgaatgg gtacgccagt ttatgcgtga actggaagag aacgcgatcg cttaatggta
    13321 aatcgggggc gtttctgcgc ccccatcggc aacatttaca aattctttgc acttccctgc
    13381 actatccgac actgtcacca tccataattc aggcttatct gtttattaca ataaccttat
    13441 atttattatg gatttttggt gatgatcaaa acgcgttttt ctcgctggct aacgtttttt
    13501 acgttcgccg ctgccgtggc gctggcgcta ccggcaaaag ccaacacctg gccgctgccg
    13561 ccagcgggca gtcgtctggt tggcgaaaac aaatttcatg tggtggaaaa tgacggtggt
    13621 tctctggaag ccatcgccaa aaaatataac gtcggctttc tcgctctgtt acaggctaac
    13681 cccggcgttg atccttacgt accgcgcgcg ggcagcgtgt taacgatccc gttgcaaacc
    13741 ctacttccag acgcgccgcg cgaaggcatt gtgatcaaca ttgcggagct gcgtctctat
    13801 tactacccgc cgggtaaaaa ttcggtaacc gtgtatccaa taggtattgg tcagttaggt
    13861 ggtgacacgc tgacaccgac aatggtgacc accgtttcag acaaacgtgc aaacccaacc
    13921 tggacgccaa cggcaaacac ccgcgcccgt tataaagcac agggaattga gttgcctgcg
    13981 gtagtgccgg ctggaccgga taacccaatg ggccatcatg cgattcgtct ggcggcctat
    14041 ggcggcgttt atttgcttca tggtacgaac gccgatttcg gcattggcat gcgggtaagt
    14101 tctggctgta ttcgtctgcg ggatgacgat atcaaaacac tctttagcca ggtcacccca
    14161 ggcaccaaag tgaatatcat caacactccg ataaaagtct ctgccgaacc aaacggtgcg
    14221 cgtctggttg aagtacatca gccgctgtca gagaagattg atgacgatcc gcagctgctg
    14281 ccaattacgc tgaatagcgc aatgcaatca tttaaagatg cagcacaaac tgacgctgaa
    14341 gtgatgcaac atgtgatgga tgtccgttcc gggatgccgg tggatgtccg ccgtcatcaa
    14401 gtgagcccac aaactctgta aatcctggat aaaaaaaatc cccgcgacac ggcatgtcgc
    14461 ggggaaaaaa tcagcagtag caggcattaa tgagggttaa tgcttattta taaattactg
    14521 ctgttccatg gagggtattc ggaccggtta cagaagtaat acggaaagat tttgcaccca
    14581 tctcatccgc tttttgcgcc agctgctctt ccagcgatcc cagatttgtc cccgctttag
    14641 cactgattgt accgactttt tgttggcctt ctggcgttga ctgaacttcg acagccgcaa
    14701 agctggcaaa tgacatggag cttaaaatcg ccgcagcgat gagggttttt acgtttttca
    14761 taatagtggc cttatgcaga tgaatgacgt cgcaagcaat tacttaacga tcgataacta
    14821 aatgacagat gtg
//
LOCUS       DAZPEB010000098        14684 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZPEB010000098 DAZPEB010000000
VERSION     DAZPEB010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14684
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.099"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..967
                     /locus_tag="ACHURJ_002528"
     CDS             <1..967
                     /locus_tag="ACHURJ_002528"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1281427.1"
                     /translation="WVGTKSLALTIPWDPKEKPNSSKCYCKDIYKDENTGDFLVMLWK
                     SDTDSTGSLLGASEDGEIGSSSVVKYTNSYRGKKVIWGRPCFYWVIPELETIVSIKFD
                     HSVCDSELFQDYVHSSITNRVKHSKRVKNKTEKGYIRLSNTDDEDLYKYMYRFDMKLR
                     SLETTHTELGKLIPKITHIVRRETIIINPNDARADWLKTFSTLVPFVSGKKNAKTRQI
                     EIKAEAKPSLNEVQEIIEKYSAEDREKRLWDNVGFATDKGITWVDKYRMRDILNVPSD
                     DYSTYSAAYIYEQISHKRKEFISPILKELKALQSQPRIKKASGED"
     gene            968..1504
                     /locus_tag="ACHURJ_002529"
     CDS             968..1504
                     /locus_tag="ACHURJ_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1281428.1"
                     /translation="MLKAIAGIAKYTWIYMAIFIISLMIISSSDINYVYNDFANYCTL
                     LVSVSGMVFTIMGIWLAFLYPNALQRIVNPKTIETVDFTEQLEDTRRLEAIVGSVLKS
                     SFVMITLLVVYLVKLVFFKSHLYLESQDVIKNTSLALIIAISYMQIEAVISVILANVM
                     FINDLHNKREQRDIDDQV"
     gene            1455..2465
                     /locus_tag="ACHURJ_002530"
     CDS             1455..2465
                     /locus_tag="ACHURJ_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1281429.1"
                     /translation="MIYITSESKETLMIKYEGKMKKNIRVIPKNIHSKLRRLGNTVVA
                     GTSIAFTENQLKSGALEHLGIYFDNGVNAYETSVIPDPLQGKYSLKNVFGEEIVRKDL
                     PKETHYTEIESPNWGDSSNGTHTVRLPYEKYPRDIIPPTLIAIEINHKQPSDSHFIFN
                     FRVTRILDKQSNKFDDELLFDLNLLQENLGKCGIEDADKPISTYADTLIVSWEIFPPG
                     SKEETLARIFRGKNITSDKKNVAENRYDFFMSLEPKKIVTGNSTFSNYIGAMLEDDLV
                     VFENIEYGNAIYILYDNWDDISKLSRIDLLSGRAGSNFDRIIHSGNWKDEVRKKVAAG
                     RL"
     gene            complement(2520..3785)
                     /locus_tag="ACHURJ_002531"
     CDS             complement(2520..3785)
                     /locus_tag="ACHURJ_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1281430.1"
                     /translation="MKLNARQVETAKPKDKTYKMADGGGLYLEVSAKGSKYWRMKYRR
                     PSDKKEDRLAFGVWPTVTLAQARAKRDQAKKLLAQGIDPKAEQKEAQAENSGAYSFET
                     IAREWHASNKRWSEDHRSRVLRYLELYIFPHIGSSDIRQLKTSHLLAPIKKVDASGKH
                     DVAQRLQQRVTAIMRYAVQNDYIDSNPASDMAGALSTTKARHYPALPSSRFPEFLARL
                     AAYRGRVMTRIAVELSLLTFVRSSELRFARWDEFDFDKSLWRVPAKREEIKGVRYSYR
                     GMKMKEEHIVQLSRQAMILLNQLKQISGDKELLFPGDHDATKVMSENTVNSALRAMGY
                     DTKTEVCGHGFRTMARGALGESGLWSEDAIERQLSHSERNNVRAAYIHTSEHLDERRL
                     MVQWWADYLQANKGKTITPYDFAKINKSR"
     gene            complement(3949..4033)
                     /locus_tag="ACHURJ_002532"
     tRNA            complement(3949..4033)
                     /locus_tag="ACHURJ_002532"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3997..3999),aa:Leu,seq:caa)
     gene            4229..5248
                     /gene="ahr"
                     /locus_tag="ACHURJ_002533"
     CDS             4229..5248
                     /gene="ahr"
                     /locus_tag="ACHURJ_002533"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFR1281431.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELRPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIKHVRDGKARYRVV
                     LKADF"
     gene            5378..6880
                     /locus_tag="ACHURJ_002534"
     CDS             5378..6880
                     /locus_tag="ACHURJ_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFR1281432.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEKQGAAHFFGEPMLDIKDWMRTDTNGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKEV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDDVDRESAYEMLQKGFQASTKQQNNPPAKGKAVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(7041..8123)
                     /gene="lptG"
                     /locus_tag="ACHURJ_002535"
     CDS             complement(7041..8123)
                     /gene="lptG"
                     /locus_tag="ACHURJ_002535"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFR1281433.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQTFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(8123..9223)
                     /gene="lptF"
                     /locus_tag="ACHURJ_002536"
     CDS             complement(8123..9223)
                     /gene="lptF"
                     /locus_tag="ACHURJ_002536"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFR1281434.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(9286..9336)
                     /gene="ytgA"
                     /locus_tag="ACHURJ_002537"
     CDS             complement(9286..9336)
                     /gene="ytgA"
                     /locus_tag="ACHURJ_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFR1281435.1"
                     /translation="MSIIILATISTTQENK"
     gene            9490..11001
                     /gene="pepA"
                     /locus_tag="ACHURJ_002538"
     CDS             9490..11001
                     /gene="pepA"
                     /locus_tag="ACHURJ_002538"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFR1281436.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFEQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAILHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            11161..11604
                     /gene="holC"
                     /locus_tag="ACHURJ_002539"
     CDS             11161..11604
                     /gene="holC"
                     /locus_tag="ACHURJ_002539"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFR1281437.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            11604..14459
                     /gene="valS"
                     /locus_tag="ACHURJ_002540"
     CDS             11604..14459
                     /gene="valS"
                     /locus_tag="ACHURJ_002540"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFR1281438.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(14515..14622)
                     /locus_tag="ACHURJ_002541"
                     /pseudo
     CDS             complement(14515..14622)
                     /locus_tag="ACHURJ_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3874 a         3525 c         3786 g         3499 t
ORIGIN      
        1 ttgggtggga actaaatctc ttgcactcac gataccatgg gatccaaaag aaaaaccaaa
       61 cagttcaaag tgttattgca aggatattta taaggatgaa aatactggtg atttcttagt
      121 catgttatgg aaatctgata ctgatagtac gggctcttta ttaggagcta gtgaagacgg
      181 tgaaatcgga agctcatcgg tggttaaata tactaatagc tacagaggga aaaaagttat
      241 ctgggggcgg ccatgctttt attgggttat acctgaactt gaaactatag tatccattaa
      301 attcgaccat tctgtttgtg attctgaact atttcaagac tatgtacact catctattac
      361 taatagagtt aaacatagca aaagggttaa aaataaaacc gagaaaggtt atataaggct
      421 atctaatacc gatgacgaag acttatataa atatatgtac aggtttgata tgaagctcag
      481 aagcttagaa accactcata ctgagttagg taaactaata ccaaaaataa cacacatagt
      541 tcgcagggaa actattatta taaatccaaa tgatgcacga gctgattggt taaagacctt
      601 tagcactctc gttccatttg tgtccggaaa gaaaaatgcc aaaacaagac aaattgaaat
      661 aaaagcagaa gcaaaaccat ccttgaatga agttcaagaa atcattgaaa agtattcggc
      721 tgaggataga gaaaaacgtc tttgggacaa tgttggattt gctacagaca agggtataac
      781 ttgggtggat aaatatagaa tgcgtgatat tctcaatgtc ccttctgacg actattcaac
      841 atattccgcc gcatatattt acgaacaaat atcacacaaa agaaaagaat ttatttcccc
      901 tatattaaaa gaattaaaag cattacaatc tcaaccaaga ataaagaaag catctggaga
      961 ggattgaata ttgaaagcta tagcaggcat agccaaatac acatggatat acatggcgat
     1021 attcattata tcgctgatga ttatttcgtc atccgatatt aactatgtgt ataacgattt
     1081 tgccaactat tgcactcttc tcgtatcagt ttctggaatg gtttttacta ttatgggaat
     1141 ttggttagcg tttctttacc caaatgcttt gcagcgtatt gtgaacccca aaactataga
     1201 aaccgttgat ttcaccgaac aactggaaga tacaagaaga ttagaagcaa tagttggaag
     1261 tgttctaaaa tcatctttcg ttatgattac tttacttgtt gtatacttag ttaaactagt
     1321 gtttttcaaa agtcatcttt atttagagag ccaagatgta ataaaaaaca catctttagc
     1381 attaattatt gctatttcgt acatgcagat tgaggcagta attagcgtaa tacttgcaaa
     1441 cgttatgttt atcaatgatt tacataacaa gcgagagcaa agagacattg atgatcaagt
     1501 atgagggaaa aatgaaaaag aatatacgtg ttatacctaa aaatattcat agcaagttaa
     1561 gaaggcttgg taatacagtt gttgctggca cttctattgc atttacggaa aaccaattaa
     1621 aaagtggagc attagagcac cttggaatat attttgataa tggtgttaat gcttatgaga
     1681 cgtcggtcat accagatcca ttgcagggta aatattcact taaaaatgtt tttggtgaag
     1741 aaattgtcag aaaagacctc ccaaaagaaa ctcattacac tgaaattgag tcacctaact
     1801 ggggtgatag ctctaatggt acacatactg ttagattacc ttacgagaaa tatcccagag
     1861 acattatacc accaacactc atagccattg aaattaatca caaacagccc tcggacagcc
     1921 atttcatatt taacttcaga gtaactagaa ttttagacaa acaatcaaat aagtttgatg
     1981 atgaattatt atttgattta aatcttcttc aagaaaatct tggtaaatgt ggaattgagg
     2041 atgctgataa acccatatcg acttatgctg atacacttat agtctcatgg gaaatatttc
     2101 caccaggtag taaagaagaa actttagcaa gaatttttag aggtaaaaat atcacttccg
     2161 acaagaaaaa tgtcgctgaa aatagatatg acttcttcat gagtcttgaa cctaaaaaaa
     2221 tcgtcacagg aaacagcacg ttcagtaatt atattggagc tatgctggag gacgatttgg
     2281 ttgtatttga aaacatagaa tatggtaatg ctatatatat cctctatgat aactgggatg
     2341 atatcagcaa gctatctcga attgacttat tatcaggaag agcaggaagt aatttcgata
     2401 gaataatcca tagtggcaat tggaaagatg aagtacgaaa aaaagttgct gctggaagat
     2461 tatgatcaaa gtgtttaata cagaaataaa cgcctatata ttataggcgt ttattttcac
     2521 tatcgacttt tgtttatttt agcaaaatca taaggtgtta ttgttttgcc cttatttgct
     2581 tggagataat cagcccacca ttgaaccatc aatcgacgtt catccaaatg ttcagaggtg
     2641 tgaatataag cagcacgtac attattacgc tctgaatggc tcaactgtcg ttctatagca
     2701 tcttcactcc ataatccgga ctcgcccaac gcaccacggg ccatcgttct aaacccgtgc
     2761 ccgcaaactt cggttttcgt gtcatagccc atcgcacgca atgcgctatt taccgtgttt
     2821 tcgctcataa ccttagttgc gtcatgatca cccggaaaaa gcagctcttt atctccacta
     2881 atctgcttta actggtttag caaaatcatc gcctgccgac taagctgaac gatatgctcc
     2941 tctttcatct tcatgccacg gtacgagtaa cgcacacctt taatttcttc tcgttttgca
     3001 ggtactcgcc agagagattt atcgaagtcg aactcatccc aacgcgcgaa acgtaactca
     3061 ctggaacgca caaaagttag taaggaaagc tcaaccgcga tccgcgtcat tacacggcca
     3121 cgatatgcag caagacgagc aagaaactca gggaaccggc tggagggcaa agcggggtaa
     3181 tgtcgcgctt tggttgtcga tagcgcaccg gccatatcac tggctggatt tgagtcgatg
     3241 taatcgttct gtactgcata acgcataata gccgtgacac gctgctgaag gcgctgtgca
     3301 acgtcatgct taccactggc atcaactttt ttaatcgggg ctaataggtg gctagttttg
     3361 agctgacgga tgtcagatga accaatatga ggaaagatat aaagctcaag ataacgtaga
     3421 acgcgtgatc ggtgatcttc actccagcgc ttattactag catgccattc acgagcgata
     3481 gtttcgaaag aatatgcccc cgaattctcg gcctgagctt ctttctgttc ggcttttgga
     3541 tcaatgccct gcgctaacag ctttttagct tgatcgcgct ttgctcttgc ctgagcaagc
     3601 gtcacagtag gccaaacacc aaacgcgaga cgatcctctt ttttgtcaga gggacgtctg
     3661 tatttcatgc gccagtattt ggaacctttg gccgaaacct caagatacaa accaccacca
     3721 tcggccattt tgtaggtttt gtcttttggc tttgcggtct cgacctgtct ggcgttgagc
     3781 ttcatttggg ggcacatttc taatcgaagt taagatgccc ccaattatgc ccccaatgat
     3841 atccggattt caacggacaa cctcggaaga cgcaggacga aaaaatcgct gcaagcattg
     3901 attttaaagg gatatttgga ctttctcgga tggtcttgga agtaagaatg gtgccgaagg
     3961 ccggactcga accggcacgt atttctacgg ttgattttga atcaactgcg tctaccgatt
     4021 tcgccacttc ggcactgaag aggtatgcgg aaaacgttgt ggattatacc tgtcgcacgc
     4081 caccatgcaa gcgccagctt gctcgcacct cgctaagtgc tgaaattttc agcattgcac
     4141 acagcgatgt gtaacctttg tcacactcca ggcaccccgc cctgccatgc tctacacttc
     4201 ccaaacaaca ccagagaagg accaaaaaat gtcgatgata aaaagctatg ccgcaaaaga
     4261 agcgggcggc gaactggaag tttatgagta cgatcccggt gagctgaggc cacaagatgt
     4321 tgaagtgcag gtggattatt gcgggatctg ccattccgat ctatcgatga tcgacaatga
     4381 atggggattt tcacaatatc cgctggttgc cgggcatgag gtgattgggc gcgtggtggc
     4441 actcgggagc gccgcgcagg ataaaggttt gcaggtcggt cagcgtgtcg ggattggctg
     4501 gacggcgcgt agctgtggtc actgcgacgc ctgtattagc ggtaatcaga tcaactgcga
     4561 gcaaggtgcg gtgccgacga ttatgaatcg cggtggcttt gccgagaagt tgcgtgcgga
     4621 ctggcaatgg gtgattccac tgccagaaaa tattgatatc gagtccgccg ggccgctgtt
     4681 gtgcggcggt atcacggtct ttaaaccact gttgatgcac catatcactg ctaccagccg
     4741 cgttggggta attggtattg gcgggctggg gcatatcgct ataaaacttc tgcacgcaat
     4801 gggatgcgag gtgacggcct ttagttctaa tccggcgaaa gagcaggaag tactggcgat
     4861 gggtgccgat aaagtggtga atagccgcga tccgcaggca ctgaaagccc tgtcggggca
     4921 gtttgatctc attatcaata ctgtgaacgt cagcctcgac tggcagcctt attttgaggc
     4981 gctgacctat ggcggtaatt tccatacggt cggtgcggtt ctcacgccgc tgtctgttcc
     5041 ggcctttacg ttaattgcgg gcgaccgcag catctctggt tctgctaccg gcacgcctta
     5101 tgagctgcga aagctgatgc gctttgccgc ccgtagcaag gttgcgccga ccaccgaact
     5161 gttcccgatg tcgaaaatta acgatgccat caagcatgtg cgcgacggta aggcgcgtta
     5221 ccgcgtggtt ctgaaagccg acttctgatc cctttgccgg gtgcctgtcg cccggcactt
     5281 aaatctcttt ggaaggcgtc tcgcaatctg ctgaatttag cggtgaagag tgcgcagccg
     5341 ttgcctatac tccaggcagg aaactggagg aaatctcatg agcgaacccc tattaattgc
     5401 ccgcacgccg gacacagaac tttttttact gccgggaatg gctaaccgtc acgggctgat
     5461 tactggcgca acggggacgg gtaaaaccgt tacgctgcaa aaactggcag agtcattgtc
     5521 ggaaatcggc gtgccggtgt ttatggctga tgtgaaaggc gatctgaccg gtatcgcgca
     5581 ggcaggaacg gcgtcggaaa aactgctcac aaggcttaaa aatatcggcg tcaatgactg
     5641 gcaaccgcat gctaatccgg tggtggtgtg ggatatcttt ggcgagaaag gccatccggt
     5701 gcgggcgacg gtttcagacc tggggccgct gttgctggcg cggctgttga atctcaacga
     5761 tgtgcaatct ggcgtgctga atatcatctt ccgtattgct gacgatcagg gattgttgct
     5821 gctcgacttt aaagatctgc gggcgattac ccagtacatc ggcgataacg ccaaatcttt
     5881 ccagaatcag tacggtaata tcagtagcgc atcggttggt gccatccagc gcggtttact
     5941 gtcgctggaa aagcaaggtg cggcgcattt ctttggcgag ccgatgctgg atatcaaaga
     6001 ctggatgcgc accgatacca acggtaaagg cgttatcaat atcctcagcg ccgagaagct
     6061 ttatcagatg ccgaaactgt acgccgccag cctgctgtgg atgctttcag agttgtatga
     6121 acaattgccg gaagcgggcg atctggagaa accaaaactg gtgtttttct tcgacgaggc
     6181 acatctgctg tttaacgatg caccgcaggt actgctggat aagattgagc aggtaataag
     6241 gcttattcgc tcaaaaggcg tgggcgtctg gttcgtttcg caaaacccgt ctgatattcc
     6301 ggataatgtg ctcgggcagc tcggtaatcg cgttcaacac gctttgcggg cttttacgcc
     6361 caaagatcag aaagaggtaa aagctgcggc gcaaaccatg cgggccaatc cggcgtttga
     6421 taccgaaaag gcaatccagg aactggggac cggcgaggcg ttaatctcgt ttctcgatgc
     6481 aaaaggaagt ccttctgtgg tggaacgggc gatggtgatt gcgccttgtt cgcggatggg
     6541 accggtgacg gaagatgagc gcaatggctt gattaaccac tccccggtgt atggcaagta
     6601 cgaggatgac gtggaccggg aatccgccta tgagatgttg caaaaaggct ttcaggccag
     6661 taccaagcag caaaataatc ctcccgcgaa agggaaagcg gtggcggtgg atgacggtat
     6721 tcttggtgga ttgaaggata ttttgtttgg cactaccgga ccacgcggcg ggaagaaaga
     6781 tggtgtggtg caaacaatgg cgaaaagcgc cgcccgccag gtgacgaatc agattgtacg
     6841 tgggatgttg gggagtttgc tggggggaag aagaaggtaa gtcaggcgag atttgtcttt
     6901 gcctgatgcg acgctggcgc gtcttatcat gcctacacca ctgcaatata ttggattttc
     6961 atgatttttc aggcatgaac gaagcacact ttgctaacaa taataaaggg agctttcgct
     7021 ccctttattc gttcattcgg ttacgatttt ctcattaaca gccacaggct gattaagaag
     7081 aagctggcgc ttggcaacag tgcgccgatg atcggcggga tgccataaac caacgtcagc
     7141 gggccgaaga tctggtccag tacgtagaag acaaaaccga agctgatacc ggtgaccaca
     7201 cgcacgccca tcggtacgct acgcagtggg ccaaagatga acgacagcgc catcagcatc
     7261 atcaccgcca cggatagcgg ctggaagatt ttgctccaca tattgagctg ataacgtccg
     7321 gcatcctgac cgctcgactt cagatacttc acgtagttgt gcaaaccgct gattgagagt
     7381 gcatccgggt ccagcgccac cacgcccagt ttgtccggcg tgaggttggt tttccaggtg
     7441 ccgctcaccg tctgcgaacc ggtgatctgt ttcggattgg tcagatcaga ttcatcaacc
     7501 tgcgacagac gccagacttt atgttccggg tcaaacttcg cagtagcggc atagcgtacg
     7561 gattgcagac gacgattctc gttaaaggca taaatactga tgccacctaa ctcttcgtca
     7621 cctttaaccc gctcaatgta aacgaagttg ttgccatctt tcgcccataa gccttgctgg
     7681 gtagagagca acgagccgcc gtacatcgcc tgcgcacggt agttacgcgc catctgctcg
     7741 ccctgcggcg cgacccattc accaatcgcc atcgtcagca agaccagcgg aatggcggtt
     7801 ttcatcaccg acagcgcaac ctgcatacgg gtgaaaccag aagcctgcat caccaccagt
     7861 tcgctgcgct gcgccagcat cccaagacca agcaacgccc caagcagagc cgccatcggg
     7921 aagaatgttt gcacatcttt cggcacgctc agcaaggtat acattcctgc gcctaacgcg
     7981 tcgtaactcc cctgcccggc ttttttcagc tgatcgacaa atttgataat gcccgacagc
     8041 gacaccagca tgaacagcgt catcatgatg gtggtgaaaa tagttttacc gatatagcgg
     8101 tcaagtacgc caaaaggttg catcacaccg ctcctttacg cgaaaaactg gcgcgcaggc
     8161 ggcggaccgg cacggtgtcc caaaggttga gaacaatcgc caaagccaga taaatcaggt
     8221 taacggtcca catccacagc gtcgggtcca gcttaccttt accgccgttc gatttcaggg
     8281 aggtctggat caggaagaaa agtagataca gcagcatggc tggcagcatc gatagtacgc
     8341 gtccctgacg cgggttaacc acgctcagcg gtacgaccat aagtgccatc ataaacacgg
     8401 tgaataccaa cgtgatacgc cagttcagtt ctgcgcgggc acgatcggtg tcagtgttcc
     8461 acaatgtacg catgtccatc tggtcggtat cgttcgggtc gagcgctacc gcctggtgac
     8521 caatgatcgc ctgataatcc tggaagtccg taatgcggaa atcacgtaac agtgcagtgc
     8581 cttcgaagcg cgttccctgg ttgagagtga cgacctggga gccgtcgcgc agctgggtta
     8641 aatgtccgga atcggccacc accacagaag gacgtgcatt accttttggt cgaatttgcg
     8701 cgaggaacac atctttgaaa tcgctgccgt caacgctttc gatgaacagc accgagctgc
     8761 cattagtcgc ttgctggaat tgcccttgcg ccagcgccgc catgccaggg ttcgctttcg
     8821 cttctgctaa cacttcatcc tgatgacgcg atgaccacgg tcccgcccac atcacgttaa
     8881 ccgccgcgac gattgcagtg aataccgcaa ggatcattgc cgctttcacc agaaccgctt
     8941 tgctcaggcc gcaggcatgc attaccgtaa tttcactttc ggtatacagt ttgcccagcg
     9001 tcatcagcag cccgaggaac aggcttaatg gcaggataag ctgcgccatt tccggcacgc
     9061 ccaacccgag aagggagagc accagattcg ccggaatatc gccgtcaacc gctgcgccga
     9121 ggatcctcac taacttttga cagaagaaga tcaaaagcaa gatgaagagt atcgccagct
     9181 ggcttttgag cgtctcccgc accagatatc ttatgattat cactttaaat acgcccgtaa
     9241 aaactcgtct tttgcaggat tttagcttgt ttcatggctt aaacgtcatt tattctcttg
     9301 agtcgtcgaa atcgtcgcta agataattat actcaacgga ttcacctctc agattttgtt
     9361 ctgacgtgcc aatgccgtaa taacgttaag attaacacga agtcatcgca acagcggaca
     9421 tgagttacga aagcttgcaa ttctatctgt agccaccgcc gttgtcttta agattcagga
     9481 gcgtagtgca tggagtttag tgtaaaaagc ggtagcccgg agaaacagcg gagtgcctgc
     9541 atcgtcgtgg gcgtcttcga accacgtcgc ctttctccga ttgcagaaca gctcgataaa
     9601 atcagcgatg ggtacatcag cgccctgcta cgtcggggcg aactggaagg aaaaccgggg
     9661 cagacattgt tgctgcacca tgttccgaat gtactttccg agcgaattct ccttattggt
     9721 tgcggcaaag aacgtgagct ggatgagcgt cagtacaagc aggttattca gaaaaccatt
     9781 aatacgctga atgatactgg ctcaatggaa gcggtctgct ttctgactga actgcacgtt
     9841 aaaggccgta acaactactg gaaagtgcgt caggctgtcg agacggcaaa agagacgctc
     9901 tacagtttcg aacagctgaa aacgaacaag agcgaaccgc gtcgtccgct gcgtaaaatg
     9961 gtgttcaatg tgccgacccg ccgtgaactg accagcggtg agcgcgcgat cctgcacggt
    10021 ctggcgattg ccgccgggat taaagcagca aaagatctcg gcaatatgcc gccgaatatc
    10081 tgtaacgccg cttacctcgc ttcacaagcg cgccagctgg ctgacagcta cagcaagaat
    10141 gtcatcaccc gcgttatcgg cgaacagcag atgaaagagc tggggatgca ttcctatctg
    10201 gcggtcggtc agggttcgca aaacgaatcg ctgatgtcgg tgattgagta caaaggcaac
    10261 gcgtcggaag atgcacgccc aatcgtgctg gtgggtaaag gtttaacctt cgactccggc
    10321 ggtatctcga tcaagccttc agaaggcatg gatgagatga agtacgatat gtgcggtgcg
    10381 gcagcggttt acggcgtgat gcggatggtc gcggagctac aactgccgat taacgttatc
    10441 ggcgtgttgg caggctgcga aaacatgcct ggcggacgag cctatcgtcc gggcgatgtg
    10501 ttaaccacca tgtccggtca aactgttgaa gtgctgaata ccgacgctga aggccgcctg
    10561 gtactgtgcg acgtgttaac ttacgttgag cgttttgagc cggaagcggt gattgacgtg
    10621 gcgacgctga ccggtgcctg cgtgatcgcg ctgggtcatc acattaccgg tctgatggcg
    10681 aaccataatc cgctggccca tgaactgatt gccgcgtctg aacaatccgg tgaccgcgca
    10741 tggcgcttac cgctgggtga cgagtatcag gaacagctgg agtccaattt tgccgatatg
    10801 gcgaacattg gcggtcgtcc tggtggggcg attaccgcag gttgcttcct gtcgcgcttt
    10861 acccgtaagt acaactgggc gcacctggat atcgccggaa ccgcctggcg ttctggtaaa
    10921 gcaaaaggcg ccaccggtcg tccggtggcg ttgctggcac agttcctgtt aaaccgcgct
    10981 gggtttaacg gcgaagagta attgcgtcag gcaaggctgt tattgccgga tgcggcgtga
    11041 acgccttatc cgacctacac agcactgaac tcgtaggcct gataagacac aacagcgtcg
    11101 catcaggcgc tgcggtgtat acctgatgcg tatttaaatc caccacaaga agccccatat
    11161 atgaaaaacg cgacgttcta ccttctggac aatgacacca ccgtcgatgg cttaagcgcc
    11221 gttgagcaac tggtgtgtga aattgccgca gaacgttggc gcagcggtaa gcgcgtgctc
    11281 atcgcctgtg aagatgaaaa gcaggcttac cggctggatg aagccctgtg ggcgcgtccg
    11341 gcagaaagct ttgttccgca taatttagcg ggagaaggac cgcgcggcgg tgcgccggtg
    11401 gagatcgcct ggccgcaaaa acgcagcagc agcccgcgcg atatattgat tagtctgcga
    11461 acaagctttg cagattttgc caccgctttc acagaagtgg tagacttcgt tccttatgaa
    11521 gattctctga aacaactggc gcgcgaacgc tataaagcct accgcgtggc tggtttcaac
    11581 ctgaatacgg caacctggaa ataatggaaa agacatataa cccacaagat atcgaacagc
    11641 cgctttacga gcactgggaa aagcagggct actttaagcc taatggcgat gaaagccagg
    11701 aaagtttctg catcatgatc ccgccgccga acgtcaccgg cagtttgcat atgggtcacg
    11761 ccttccagca aaccatcatg gataccatga tccgctatca gcgcatgcag ggcaaaaaca
    11821 ccctgtggca ggtcggtact gaccacgccg ggatcgctac ccagatggtc gttgagcgca
    11881 agattgccgc agaagaaggt aaaacccgtc acgactacgg ccgcgaagct ttcatcgaca
    11941 aaatttggga atggaaagcg gaatccggcg gcacgattac ccgtcagatg cgccgtctcg
    12001 gcaactccgt cgactgggag cgtgaacgct tcacgatgga cgaaggcctg tccaatgcgg
    12061 tgaaagaagt tttcgttcgt ctgtataaag aagacctgat ttaccgtggc aaacgcctgg
    12121 taaactggga tccgaaactg cgcaccgcta tctctgacct ggaagtggaa aaccgcgaat
    12181 cgaaaggttc gatgtggcac atccgctatc cgctggctga cggcgcgaaa accgcagacg
    12241 gtaaagatta tctggtggtt gcgactaccc gtccggaaac cctgttgggc gataccggcg
    12301 tggccgttaa cccggaagat ccgcgttaca aagacctgat tggcaaatat gtcattctac
    12361 cgctggttaa ccgtcgtatt ccgatcgttg gcgacgaaca cgccgacatg gaaaaaggca
    12421 ccggctgcgt gaaaatcact ccggcgcacg actttaacga ctatgaagtg ggtaaacgtc
    12481 acgcgctgcc gatgatcaac atcctgacct ttgacggcga tatccgtgaa agcgcccagg
    12541 tgttcgatac caaaggtaac gaatctgacg tttattccag cgaaatcccg gcagagttcc
    12601 agaaactgga gcgttttgct gcacgtaaag cagtcgttgc tgcggttgac gcgctcggcc
    12661 tgctggaaga aattaaaccg cacgacctga ccgtgcctta cggcgaccgt ggcggcgtag
    12721 ttatcgaacc gatgctcacc gaccagtggt acgtgcgtgc cgatgtactg gcgaaaccgg
    12781 cggttgaggc ggttgagaac ggcgacattc agttcgtacc gaagcagtac gaaaacatgt
    12841 acttctcctg gatgcgcgat attcaggact ggtgtatctc tcgtcagctg tggtggggtc
    12901 accgtatccc tgcatggtac gacgaagcag gtaacgttta tgttggccgc aacgaagaag
    12961 aagtgcgcaa ggaaaataac ctcggtgccg acgttgccct gcgtcaggac gaagacgttc
    13021 tcgacacctg gttctcttct gcgctgtgga ccttctctac cctaggctgg ccggaaaaca
    13081 ccgacgcact gcgccagttc cacccaacca gcgtgatggt atctggtttc gacatcattt
    13141 tcttctggat tgcccgcatg atcatgatga ccatgcactt catcaaagat gaaaatggca
    13201 aaccgcaggt gccgttccac accgtttaca tgaccggcct gattcgtgac gacgaaggcc
    13261 agaagatgtc caaatccaag ggtaacgtta ttgacccgct ggatatggtt gacggtattt
    13321 cgctgccgga actgctggag aaacgtaccg gcaatatgat gcagccgcag ctggcggaca
    13381 aaatccgtaa gcgcaccgag aaacagttcc cgaacggcat tgagccgcac ggcaccgatg
    13441 ccctgcgctt caccctggcg gcgctggctt ctaccggtcg tgacatcaac tgggatatga
    13501 aacgcctgga aggctaccgt aacttctgta acaagctgtg gaacgccagc cgctttgtgc
    13561 tgatgaacac ggaaggtcag gattgcggct tcaacggtgg tgaaatgacg ctgtcgctgg
    13621 cggaccgctg gattctggca gagttcaacc agaccatcaa agcgtaccgc gaagcgctgg
    13681 acagcttccg cttcgatatc gctgcaggca ttctgtatga gttcacctgg aaccagttct
    13741 gcgactggta tctggagctg actaagccgg taatgaacgg tggaaccgaa gccgaactgc
    13801 gcggtactcg ccatacgctg gtgactgtac tggaaggtct gctgcgcctc gcgcatccga
    13861 tcattccgtt catcaccgaa accatctggc agcgtgtgaa agtactttgc ggtatcactg
    13921 ccgacaccat catgctgcag ccgttcccgc agtacgatgc atctcaggtt gatgaagccg
    13981 cactggctga caccgagtgg ctgaaacagg cgatcgttgc ggtacgtaac atccgtgcag
    14041 aaatgaacat cgcgccgggc aaaccgctgg agctgctgct gcgtggttgc agcgcggatg
    14101 cagaacgtcg cgtaaatgaa aaccgtggct tcctgcaaac cctggcgcgt ctggaaagca
    14161 tcaccgtgct gcctgccgat gacaaaggtc cggtttccgt tacgaagatc atcgacggcg
    14221 cagagctgct gatcccgatg gctggcctca tcaacaaaga agatgagctg gcgcgtctgg
    14281 cgaaagaagt ggcgaagatc gaaggcgaaa tcagccgtat cgagaacaag ctggcgaatg
    14341 aaggctttgt cgcccgcgca ccggaagcgg tcatcgcgaa agagcgtgag aagctggaag
    14401 gctatgcaga agcgaaagcg aagctgattg aacagcaggc tgttatcgcc gcgctgtaat
    14461 caaaacacag tgatgaaaac gaaggccgga gcatgctccg gcctttttta tctcttacag
    14521 aaatggtaaa gaaggcataa ttccccgtga aatactggct aagaagcctt tatctggttg
    14581 ttcttcttta ttgattaaag gaaggtcggt aatgctgcca acttactgat ttagtgtatg
    14641 atggtgattt taaggtgctt gcgtggcttc catttccatc agat
//
LOCUS       DAZPEB010000099        14678 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZPEB010000099 DAZPEB010000000
VERSION     DAZPEB010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14678
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.100"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..319)
                     /locus_tag="ACHURJ_002542"
     CDS             complement(<1..319)
                     /locus_tag="ACHURJ_002542"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1281439.1"
                     /translation="MSVARFSCGKTAQLSKKQTGYYSPEIFPSTGKDCNPQPANCLKD
                     QYVLRHCCVDDRSGKMGYSVKFLVLTRMDTETASLFHCKPCYSKMTFTIYHPLTHSFY
                     MNAS"
     gene            454..3639
                     /gene="rne"
                     /locus_tag="ACHURJ_002543"
     CDS             454..3639
                     /gene="rne"
                     /locus_tag="ACHURJ_002543"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFR1281440.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPAAPA
                     QPGLLSRFFGALKALFSGSEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPHHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(3836..4789)
                     /gene="flgL"
                     /locus_tag="ACHURJ_002544"
     CDS             complement(3836..4789)
                     /gene="flgL"
                     /locus_tag="ACHURJ_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFR1281441.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFAIQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEEKGKYVGGAESI
                     KQQVDASRSMVIGHAGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLAVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(4801..6444)
                     /gene="flgK"
                     /locus_tag="ACHURJ_002545"
     CDS             complement(4801..6444)
                     /gene="flgK"
                     /locus_tag="ACHURJ_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFR1281442.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNKGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEKDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSATQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(6510..7450)
                     /gene="flgJ"
                     /locus_tag="ACHURJ_002546"
                     /pseudo
     CDS             complement(6510..7450)
                     /gene="flgJ"
                     /locus_tag="ACHURJ_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
     gene            complement(7450..8547)
                     /gene="flgI"
                     /locus_tag="ACHURJ_002547"
     CDS             complement(7450..8547)
                     /gene="flgI"
                     /locus_tag="ACHURJ_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFR1281443.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSARLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(8559..9257)
                     /locus_tag="ACHURJ_002548"
     CDS             complement(8559..9257)
                     /locus_tag="ACHURJ_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFR1281444.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVVDARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(9310..10092)
                     /gene="flgG"
                     /locus_tag="ACHURJ_002549"
     CDS             complement(9310..10092)
                     /gene="flgG"
                     /locus_tag="ACHURJ_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFR1281445.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMQQKLTQL"
     gene            complement(10230..10985)
                     /gene="flgF"
                     /locus_tag="ACHURJ_002550"
     CDS             complement(10230..10985)
                     /gene="flgF"
                     /locus_tag="ACHURJ_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFR1281446.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKIDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHQVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(11005..12213)
                     /gene="flgE"
                     /locus_tag="ACHURJ_002551"
     CDS             complement(11005..12213)
                     /gene="flgE"
                     /locus_tag="ACHURJ_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFR1281447.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGAINGAEPATFSLSFLNSMQQNTGANNIV
                     ATPQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWS
                     ATQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQD
                     QILNTLVNLR"
     gene            complement(12238..12933)
                     /gene="flgD"
                     /locus_tag="ACHURJ_002552"
     CDS             complement(12238..12933)
                     /gene="flgD"
                     /locus_tag="ACHURJ_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFR1281448.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTMLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAITASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(12945..13349)
                     /gene="flgC"
                     /locus_tag="ACHURJ_002553"
     CDS             complement(12945..13349)
                     /gene="flgC"
                     /locus_tag="ACHURJ_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFR1281449.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(13353..13682)
                     /gene="flgB"
                     /locus_tag="ACHURJ_002554"
     CDS             complement(13353..13682)
                     /gene="flgB"
                     /locus_tag="ACHURJ_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFR1281450.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTLGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYQMSLSALSGQIKGMM
                     NVLQSGN"
     gene            13838..14497
                     /gene="flgA"
                     /locus_tag="ACHURJ_002555"
     CDS             13838..14497
                     /gene="flgA"
                     /locus_tag="ACHURJ_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFR1281451.1"
                     /translation="MLAIKRSVAIIAILFSPLSAASNLTSQLHTFFSAQLVGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAISLRDLSPDQPIQLTHFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARMRMVSGLVVSGVVDADGNILIN
                     L"
     gene            14573..>14678
                     /locus_tag="ACHURJ_002556"
     CDS             14573..>14678
                     /locus_tag="ACHURJ_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020899.1"
                     /note="regulates the flagellar specific sigma28
                     transcription factor; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HFR1281452.1"
                     /translation="MSIDRTSPLNPVSTVQPRETTDAPVTNTRAAKTTD"
BASE COUNT         3333 a         4001 c         3825 g         3519 t
ORIGIN      
        1 gggatgcgtt catataaaaa ctgtgagtaa gcgggtgata aatggtaaaa gtcatcttgc
       61 tataacaagg cttgcagtgg aataatgaag ccgtttccgt gtccatcctt gttaaaacaa
      121 gaaattttac ggaataaccc attttgcccg accgatcatc cacgcagcaa tggcgtaaga
      181 cgtattgatc tttcaggcag ttagcgggct gcgggttgca gtccttaccg gtagatggaa
      241 atatttctgg agagtaatac ccagtctgtt tctttgataa ttgcgctgtt tttccgcatg
      301 aaaaacgggc aaccgacact ctgcgcctct ttgagctgac gataaccgtg aggttggcga
      361 cgcgactaga cacgaggcca tcggttcaca cccggaaagg cgttactttg cccgcagctt
      421 agtcgtcaat gtaagaataa tgagtaagtt acgatgaaaa gaatgttaat caacgcaact
      481 cagcaggaag agttgcgcgt tgcccttgta gatgggcagc gtctgtatga cctggatatc
      541 gaaagtccag ggcacgagca gaaaaaggca aacatctaca aaggtaaaat cacccgcatt
      601 gaaccgagtc tggaagctgc ttttgttgat tacggcgctg aacgtcacgg tttcctccca
      661 ctaaaagaaa ttgcccgcga atatttccct gctaactaca gtgctcatgg tcgtcccaac
      721 attaaagatg tgttgcgtga aggtcaggaa gtcattgttc agatcgataa agaagagcgc
      781 ggcaacaaag gcgcggcatt aaccaccttt atcagtctgg cgggtagcta tctggttctg
      841 atgccgaaca acccgcgcgc gggtggcatt tctcgccgta tcgaaggcga cgaccgtacc
      901 gaattaaaag aagcactggc aagccttgaa ctgccggaag gcatggggct tatcgtgcgc
      961 accgctggcg tcggcaaatc tgctgaggcg ctgcaatggg atttaagctt ccgtctgaaa
     1021 cactgggaag ccatcaaaaa agccgctgaa agccgcccgg ccccgttcct gattcatcag
     1081 gagagcaacg taatcgttcg cgctttccgc gattacttac gccaggacat cggcgaaatc
     1141 cttatcgata acccgaaagt gctcgaactg gcacgtcagc atatcgctgc attaggtcgc
     1201 ccggatttca gcagcaaaat caaactgtac accggcgaga tcccgctgtt cagccactac
     1261 cagatcgagt cgcagatcga gtccgccttc cagcgtgaag tccgtctgcc gtccggtggt
     1321 tcgattgtta tcgacagtac cgaagcgtta actgccatcg acatcaactc cgcacgcgcg
     1381 acccgcggcg gcgatatcga agaaaccgcg tttaacacca acctcgaagc tgccgatgag
     1441 attgctcgtc agctgcgcct gcgtgacctc ggcggcctga ttgttatcga cttcatcgac
     1501 atgacgcctg tacgccacca gcgtgcggta gaaaaccgtc tgcgtgaagc ggtgcgtcag
     1561 gaccgtgcgc gtattcaaat cagccatatt tctcgctttg gcctgctgga aatgtcccgt
     1621 cagcgcctga gcccatcact gggtgaatcc agtcatcacg tttgtccgcg ttgttctggt
     1681 actggcaccg tgcgtgacaa cgaatcgctg tcgctctcta ttctgcgtct gatcgaagaa
     1741 gaagcgctga aagagaacac ccaggaagtt cacgccattg ttcctgtacc aatcgcttct
     1801 tacctgctga atgaaaaacg ttctgcggta aatgccattg aaactcgtca ggacggtgtg
     1861 cgctgcgtga ttgtgccaaa cgatcagatg gaaaccccgc actaccacgt gctgcgcgtg
     1921 cgtaaagggg aagaaacccc aaccttaagc tacatgctgc cgaagctgca tgaagaagcg
     1981 atggcgctgc cgtctgaaga agagttcgct gaacgtaagc gtccggaaca acctgcgctg
     2041 gcaacctttg ccatgccgga tgtgccgcca gcgccaacgc cagctgaacc tgccgcgcct
     2101 gttgtagctc cagcaccgaa agctgcaccg gcaacaccag cagctcctgc acaacctggg
     2161 ctgttgagcc gcttcttcgg cgcactgaaa gcgctgttca gcggtagtga agaaaccaaa
     2221 ccgaccgagc aaccagcacc gaaagcagaa gcgaaaccgg aacgtcaaca ggatcgtcgc
     2281 aagcctcgtc agaacaaccg ccgtgaccgt aatgagcgcc gcgacacccg tagtgaacgt
     2341 actgaaggca gcgataatcg cgaagaaaac cgtcgtaatc gtcgccaggc acagcagcag
     2401 actgccgaga cgcgtgagag ccgtcagcaa gctgaggtaa cggaaaaagc acgtaccacc
     2461 gacgagcagc aagcgccgcg tcgtgaacgt agccgccgcc gtaacgatga taaacgtcag
     2521 gcgcaacaag aagcgaaggc gctgaatgtt gaagagcaat ctgttcagga aaccgaacag
     2581 gaagaacgtg tacgtccggt tcagccgcgt cgtaaacagc gtcagctcaa tcagaaagtg
     2641 cgttacgagc aaagcgtagc cgaagaagcg gtagtcgcac cggtggttga agaaactgcc
     2701 gctgccgaac caattgttca ggaagctcca gctccacgca cagaactggt gaaagtcccg
     2761 ctgccagtcg tagcgcaaac tgcaccagaa cagcaagaag agaacaatgc tgataaccgt
     2821 gacaacggtg gcatgccgcg tcgttctcgc cgctcgcctc gtcacctgcg cgtgagtggt
     2881 cagcgtcgtc gtcgctatcg tgacgagcgt tatccaaccc agtcgccaat gccgttgacc
     2941 gtagcgtgcg cgtctccgga actggcctct ggcaaagtct ggatccgcta tccaattgta
     3001 cgtccgcaag atgtacaagt tgaagagcag cgcgaacagg aagaagtaca agtgcagccg
     3061 atggtgactg aggtccctgt cgccgccgct gtcgaaccgg ttgttagcgc gccagttgtt
     3121 gaagaaatgg ctgaagtcgt agaagccccc gtaccggttg ccgaaccgca accggaagtg
     3181 gttgaaacga cgcatcctga agtgattgcc gccgcggtaa ctgaacagcc gcaggtgatt
     3241 accgagtctg atgttgccgt agcccaggaa gttgcagaac acgcagaacc ggtggttgaa
     3301 ccgcaggaag agacggcaga tattgaagaa gttgccgaaa ctgctgaggt tgtggttgca
     3361 gaacctgaag ttgttgctca acctgccgcg ccagtcgtcg ctgaagtcgc agcagaagtt
     3421 gaaacggtag ccgcggttga accagagatc accgttgagc ataaccacgc taccgcgcca
     3481 atgacgcgcg ctccggcacc ggaatatgtt ccggaggcac cgcatcacag tgactggcag
     3541 cgccctactt ttgccttcga aggtaaaggt gccgcaggtg gtcatacggc aacgcatcat
     3601 gcctctgccg ctcctgcgcg tccgcaacct gttgagtaat aattagctca aagtaatcaa
     3661 gccctggtaa ctgccagggc ttttttattt catgtttgaa tcaacatatt aaaattcatc
     3721 aaatggcatc cttgctaacc aacaatgcaa aataggcaaa aaaaatgccc gccccggcga
     3781 tccctgagcg ggcagacaaa acatacccag tttcatgata tgttttaaag cgagcttatt
     3841 tgttgagctg gaagagcgac aatccctgca tatcggtaaa tgctttatac gatgcctgca
     3901 atgccgtttg ctgcatgatg taagatgaaa tagttgcatt ccagtccaca tcaaccagat
     3961 cgctcatctg ctgcgtttgc cctaaagcgc gatcgctacc taaggaatcc agcgactcca
     4021 gttcgttcag ctgcgtgcct aattccgcgc gaacggcaag gacattattc agcgagtttt
     4081 tcagtccacg gttggtttta tccaatgccg cagcggcgat ttctttatcc gcttcgctat
     4141 ccgcgaccgg cgttttcagg gctgcgatgg cgctatccag catggcaaaa agattggttt
     4201 cagaagcgct accgtctggc tccgcaactg cgttgctggt aatactgtcg aaaattttgt
     4261 cacccgcgtg ccctatcacc atcgaacgcg aagcatcgac ctgttgctta atactttctg
     4321 ctccaccgac gtatttccct ttctcttcgc taaacggcgc agtctctgtt ttataaccgg
     4381 caaaaatgta gcgcccgtta ccgtcagtgg tgttcgccag attcagcaac tggtcacgaa
     4441 gcccctgaat atccgtagcc agcgaggccc ggtcatcgtc actcaaggtg ccattgctgg
     4501 cgtagacaat tttttcctga gcattctgga tagcagtggt gacctggcta agtacactct
     4561 cttccagtga cactttttga atggcgaaag tacgcgccag cgtgtactgg ctgttttgcg
     4621 cctgtgcctg ggagagaact acggcttgtg atgctgcaat gggatcgtca gaagggttaa
     4681 cgactcgctt acctgtcgac atctgctcgc cgtacttcat ccattccgcc tgagaattgg
     4741 tgatgccacg catgttttgc tggtacatca tctgggtact gaaacgcatc ttatctcccc
     4801 ttagcgaatg ttaatcagcg catcaaaaat cgcgtttgcc gtctgcagaa cctgcgcatt
     4861 cgccaggtaa tactgctgaa aacgttgcag atttccgtac tcttcgtcga gattgacgcc
     4921 agagatagac tgctgctgat tggaaagctg cgtcaccaca ttaccttgcg tggcgctact
     4981 ggttttcaac gtcgcggttt tattaccgat atcactcact aacgaggcat aagcgtcgtt
     5041 aaaggatttc gcaccgccca ccgttttact gttgctttgc agatccagca gggcctgacc
     5101 gttgcggtta tcgctatcac ccgcatcttt ttcgctcgcc atcgctattt tcgcttcgtc
     5161 ggtgattaat acatccatgt tgacgatggc gtcacttact ggtttcagcg tgaagctgtc
     5221 gttaacggca ggcgttcctg taaacgtcaa ctccagacca tcaaatgcca ctttaccgtt
     5281 ggcatccggc gtcaccgtaa aagtggtatt gctggcaagg cgggtgacct gccactgatt
     5341 attatcgaac gagattttgt aatctgtcgc cagtaccgcg gaggcatcag ttaccgtggc
     5401 accgatcgca acgtcacctt tgtttttcgt gttttgcaaa accgcgggct taccgatagc
     5461 aaagaaatct tcaccagcat cgccgttggc atcaaatccg gctttgtgtt gggtgttgaa
     5521 agcctcagca aatgccagcg ccagttgtcc aagcgtatta cgcgtctggt ccagatcctg
     5581 agaacggaat gtcagaatgc cgcccagcga cccggtattc agtaatttct ccgggatctc
     5641 aatattgcct gccgtcccat caacgtaagc gacagtcgta cgagaagggt cggcgctgga
     5701 aggaactgcc gccagttgcc gcgccgtact tccctgaacc agtgagtaac cattggccat
     5761 cgtgatgtta taagtgccgc catcctgaac gctgacttct acaccaacaa tctggtttaa
     5821 ttcgctcacc agttgatcgc gttgatccag cagattgtta ggtgacgccc ctgcccccac
     5881 gcctgtcagg cgggagattt ggtcgttcag gctggcgatt tgtttagcgt aattgttgat
     5941 ctgatcaacg ctggcaccta tcgcgatatt gacctgtttg tcctggtcgc gaagatactg
     6001 atcggtggtt ttaaactgat tcaccaaccc ttctgatttc ccaatcagcg cctggcgcgc
     6061 tgccgggtct tccgcgttac tcaccagcgt ttgcaggctg gtgaagaaat cctgcatctg
     6121 tgttgccagc gaagaggtac tggtggagag catattgtcg attttcgaca tctgctcata
     6181 gcgggcagtc aggccgctac tttgcgtctg cgccgcacgt aactggttgg tgataaacgc
     6241 atcatactca cgctgcacac cagaaacgta gacaccattg ccaacccagc cgccagcgcc
     6301 caacgtgcta ttggcctgcg ccataatagt ggtttggcgg gtatatccgg caacgttata
     6361 gctggagata ttattacttg ccgtatttaa cgccgcctgg gccgcgttca gtccgctcat
     6421 ggcgttatta atcaagctgg acatggaggt tccttataag ccttcaatta cagagtatta
     6481 tcggcagcga cccgccggac ttgagttatt caaaacagat tatcaatgtt catactgtag
     6541 gttttgctca ccttgtcgct tatcgatttc atctgctgaa tcatgttggt gagtttgcgg
     6601 gcatagtgag gatcggtggc atagcccgcg tcctgtaggg cctgcgcccc cgctccgcac
     6661 tcgtggcggt ggtcacggcg gcgtagcgcg ggttacgcgt taacagccca acgtaatccg
     6721 actatgcttc cagatacgag ctgtagaccc gaaacttcgc ttttactttc ttcgcttcgc
     6781 cgttttcata ttcagtcgtg gtgatttcag taactggccc tttccagttg ccagaggctt
     6841 tgacaccaaa caggttatag ctcggctcgc cgttttcgcg gcggatttgc cgttgccccc
     6901 aaccagattc cagcgccgcc tgagcgagga tcaaatgatg tggcacaccg ctttgctggc
     6961 ttgccagttg ggcgggcagc gagagttgcg cgaggaatgc tttactgtca cccggcagcg
     7021 aatcatcgta gttacgtggc acggcctttt gcaccagctg cgaaagcgcc tgattttgat
     7081 aacgcaccac agtttcgagc gggaatttca tcggtgctgc tggcgtggac tcctctggca
     7141 atggttgttc tggcgtcatc tgtttaacca tcatctctgc aagccccaga cctttgcccg
     7201 ccgtcatctg ttgggcaatc tgctggtcat acatactggt atacaggcga gtgtgctcgc
     7261 tgctgaacag gccatctttt ggtaaagcgt cgcgcatgct tttcaacatc atctgcacga
     7321 acatcccttc cacctgacgg gccaccggac ggatatttgc cgccggatct tcgcccgctt
     7381 tcgcctttag ttcgttgagt gattgcgcat cccaggccgc acttgccagt agtttgctat
     7441 cgctgatcat cagatgattt ccagttttgc ccgcagacat cccgcacttt gcattgattg
     7501 cagtatggac atcagatcca tcggcgtagc gcccagcgca ttgagcgcgc gcaccacgtt
     7561 attgagcctg gcgctggaac gtacgctttg cagcgaaccg ccgctctggc gtaaatcgat
     7621 ctgcgtttgt ggagtaacca cagtctgtcc accaccaaac ggtgtatctg gctggctgac
     7681 attggcctga cgattaactg ttactgagag attcccctgc gctaccgcgc agctgtcgag
     7741 ggtcacttcg cgattcatca ccaccgaacc ggtgcgcgag ttaatcacta ctttagcgtc
     7801 ctgcggggtg acattaacct gcatattctg gatatcggca aggaagcgga cctgggaact
     7861 gttgccactc ggtacgcgca cctgaatagt ccgcgcatct aacgcggtgg cgctgccata
     7921 tccacgcacg cggttgatgg tgtcagcgat ttgctgcgcc atgctgaaat cttcgtcgtt
     7981 aagttgcaaa ttaagggtat tcccgacgcc aaactggctg ggcaattcac gttcaataac
     8041 cgcaccattg gtgatccgtc caccgttcag ttggttaacc tgaacactgc taccgccagc
     8101 ggaggctcct gcgccgccaa ccagaatatt gccctgcgcc agcgcataca cctgactgtc
     8161 aacgccctta agcggtgtca tcaacaacgt acctccacgc aagcttttgg cattacccat
     8221 ggaagaaacc accacatcga tggtttgccc ctgacgtcca aacggaggaa gtgacgctgt
     8281 caccattacc gcagcgacgt tttttagctg catattggtg cccgtcggaa cggtaattcc
     8341 cagctgtgag agcatgttat taagcgtttg tgtggtaaac ggcgtctggg ttgtctggtc
     8401 accggtgcca tccagcccca ccaccagacc atagccaatc agtgagtttt gccttacccc
     8461 ctgaacactg gtgagatcgc gaatacgctc agcctgagcc gccgtcgtga ccagtagaag
     8521 aattaatgca gagagaaatt taatcaccac agcctcactt acattggcga caggttaagg
     8581 aagaaacgct gcaaccagcc catattttgc gcttcgttaa tgtagccatt gcctacgtat
     8641 tcaatgcgcg catccaccac ctgagtagac ggtacggtat tgctgccgct gatagtgcgt
     8701 ggattaacca cgccagagaa gcgaataaat tcggtaccct gattaatggc aatctgtttt
     8761 tcacccacca catgcaggtt gccgttgacc agtacctggt caaccgtcac cgtcaacgtg
     8821 ccgctaaagg tattgctggc attggccccg ccctttccgt tgaacgtgtt accaccggag
     8881 gcttcgacat cggcacgagc gttaccaaac agcccctgca aatagcgcgg cacagtatca
     8941 aagccaaaat tagttttacc gtcacggctg gcattcgcag aggagctttt gctggcgctg
     9001 acgttctcct gcaacacgat ggtcagcgta tcgccaatat tgcgtggtcg acgatcttca
     9061 aacagcggtt gatagccata gttaatcggc tgagcagact ggaaaataga accgttggcg
     9121 acgggcgtcg gaccgggaac cggttgtgca ctggtcgccc cctgcaccag cggcgtggag
     9181 ggtatccagg cgcagccggt tagtgaaagc accaacaagc tggaaatggc ataagtatgc
     9241 gcagcgtttt tttgcatggc tatcatcttc aaaaatcagt aaaccggtga acctgccacc
     9301 ggttaagcct tagagttgcg tcagtttttg ctgcatctga tcggtggtgg acaccgcttt
     9361 actgttgatt tcgtaagcgc gttgcacctg aatcatattg accagttctt ccgccacgtt
     9421 gacgttagac gtttcaacat acccttgata cagcaatccc gcgccgttca ggcccggcgt
     9481 gctttcgttc ggtgcaccag aggattgcgt ttcggtgtag aggttttcgc caatgctctc
     9541 cagcccggtg tcattcataa aggtggtgag attgagctgc ccaacctgaa ccggagctgc
     9601 ctggccttgt tgggttacgc tgaccacgcc atcacgaccg atggtgatac ttaacgcatt
     9661 cgccggaatg gtgatcgctg gctgcacctg aaaaccacca gccgtcacca gctgcccgtt
     9721 ctgatccacc tggaaagagc cgtcacgggt ataggctgat gaaccatctg gcaacatcac
     9781 ctggaaaaag ccctgccctt taatcgcgac atctttgctg ttgttggtct gcgacagatt
     9841 tccctggctg tgtaagcgtt cagtggcgac cgggcgtacc cccgtgccga tttgtaatcc
     9901 ggagggtaag gtggtttgtt cggaagactg tgcccccggc tggcgaatgg tttgataaag
     9961 cagatcttca aacaccgcgc gctgacgctt aaaaccgtta gtactgacgt ttgccaggtt
    10021 gttggcaatg acgtccatat tggtttgctg ggcgtcaagg cccgttttgg cgatccataa
    10081 tgaactgatc ataaaatgtc ctgtagtgga ttagtgacaa gcagagcaga agcacgcgac
    10141 agtgtgaatt ccccctcact ctaaccctct ccccaaaggg gcgagggaat agtccgcgcg
    10201 cttttattgc aatgtcatca atcgttcaat taactcatcg acagcagttg gttggcacgg
    10261 cctgcgttat catcgacgct gctgatcacc ttcatctgca tttcaaaacg ccgcgcgctg
    10321 gcaatcatgt cgctcattgc cgcaacggca ttgacgttac tgccttccag aacccccgac
    10381 atcacacgca aggttggatc tgcctgcagt accggccctc gcgtggcctg ggtttctgcg
    10441 cttaaacgaa aaatgccgtc gtcaccgcgc tgcacttcgc tgcccgtggc tttcaccagt
    10501 ttcagacgcc ctactggcgc aactgtattt gccggatcgc ccggattgag cgccgagatt
    10561 gtgccatcgg cagcaatagt gatttccgct ccttccggca cagcaattgg ccccgcctcg
    10621 cctatcacct gatgcccctg aattgtcagt tgcccggtgg gatcaacctg aatgctgcca
    10681 ttacgcgtat acccttcgct gccgtcagcg gtctgcacgg ccagccagcc atcctgctgc
    10741 aacgcaacgt ccagcgggcg cgaggtgtaa tctatcttgc cgggcgtcat gtctgcaccc
    10801 ggtgttgacg ccgttaccag cgtgcgcgtg ggcagtgaaa gcccttccac cggcactgcg
    10861 cgcaaggcgt ttaactgcgc gcgaaaaccg ggcgtagagg cattggccag attactggcg
    10921 gttaccgcct gttgattcag cgtctggctg gctgctccca tcgcagtata aattgcgtga
    10981 tccattgagc tatcccgtca gcgattagcg taagttaacc agcgtgttga ggatctggtc
    11041 ctgggttttg atggtctggg cgttagactg atagttacgc tgggcaacga tcatattgac
    11101 cagttcttta ctgagatcga cgttggacgc ttccagcgca ccgttggtca gggtgccaaa
    11161 gtttcccgtc ccggctgtcc ccaacagcgc cacgccagaa gattgcgtcg cagaccagac
    11221 gttgtcgcct tcggatgcca gaccttcgtt gttggcaaag ttcgccagta caatctgccc
    11281 cagcagttga gtttgttcgt tggaatagtt gccgacaacc gtaccgtcat cattgatttg
    11341 ataactcacc agatcgcccg gtttgtagcc gttctggggg gttgccacaa tattgttagc
    11401 gccggtattt tgctgcatgg agttgaggaa gctcagacta aacgtggcgg gttctgcacc
    11461 gttaattgcg ccggttgcga tattattcgc catcgcacca tccactaatg tgccattagc
    11521 attaaattcc agtgttgtcg ctgtcttcgc aatgctgttt ggatcactgc tatcctgggt
    11581 gtagacctgc cagttattat ccccggtctt cacaaagtag acgctcatgt catgagcatt
    11641 accctgactg tcgaaaacag tcaccgaacc ttttttgtta tagctatccg cattgctggc
    11701 gctgaatggc gtaacagtag gaagcggatc actggaattc aggttgatct gcatcgacgc
    11761 cgtggtggta gttttcgctg ccatcagggt attcgggatc gaaatattgg tcggattcgc
    11821 cccttgctga atagtcggcg gcgtaccggt tgccgggtaa cccgtcagct gtaaaccttg
    11881 catattcacc aggttacggt tttcatccag cttaaattgt ccgttacggc tgtagaacac
    11941 cgaaccgttg ctgtctacca gacggaaaaa accgttctgg ctgatagcaa cgtccagacc
    12001 tcgcccggtg ttggtggtcg tgccatctgt aaagtcctga gtgataccgg caacttttac
    12061 ccccagtccc actttcgaac cggcaaacat atcggcaaaa gaggccgtgc ctgatttaaa
    12121 gccgtaggtg gcggagttgg caatattgtt gccaataaca tcgaggttag tggcggcagc
    12181 gtttaatccg ctaaccgctt gagaaaaggc catgactgac tcctgaaatg tgaaggctta
    12241 aattatctgc cgtacttcgt cgagggtggt ggtgccgtaa gtgccgagat ccagcgtatt
    12301 accgctgttg ccgcggatca caccctgcac cagagcaaac tgcagcggct gggcaaccag
    12361 ttgtgtacca ccgttactgg cggtaatcgc tacattgtaa gaaccgttcg gcgcagtgct
    12421 gccatcagtc aacgtaccgt cccaggtgaa actgtgaact ccggcggtca gttcaccaat
    12481 atcaatggtg cgcacaaccg cgccattttt atcggtgatg gtggccgtaa ctttgtctgc
    12541 cgcctgttgc agctcaacac caaacggcgt ggtcgtggtc acagcccctt cttcactgcc
    12601 ggttcccgca agaacagttg tgccggggat catcacgccg tgaccgatca ggttactggc
    12661 ctgtaacgac tggctgttat caatctgtcc ggaaatagat ccgagcatgg tattgagttt
    12721 ttcaatcccg ctgaccgtgc tgatttgtgc caattgcgac gtcagctcgt tgttttccat
    12781 tggattggtc gggtcctggt ttttcagctg cgccaccagc aaagtcagaa aactgctttg
    12841 taaatctgcg gcgttgctgc ccgtgagcga actactgctg gtggtactga cgccggtatt
    12901 tgtcggatcg gtggtggtta ccgcaatgga catagctttc tcctttattg accgagcgta
    12961 agggttttca gcatcatgct tttcaccgtg ttgagcactt caacattggc ctgatagctg
    13021 cgtgacgccg acatggtgtt aaccatctct ccgacaacat caacgttcgg catttttacg
    13081 tagccctttg catccgccag cggattaccc ggttcataaa ccagtttgtc cggggcctga
    13141 ctttctataa catcggcaac ctttacgccg cctgtcgcag cacctggtgc agcgttaacc
    13201 tggaatacca cctgttttgc ccgatatggc tgtccatcgg gaccggtcac gctatcagca
    13261 ttcgccagat tactggccgc cacgttcagg cgctgggact gggcagttaa cgccgacccg
    13321 gcgatatcaa aaatattcag cagtgccatc cgttaatttc cgctctgtaa aacgttcatc
    13381 atgcctttga tttgcccgct caacgcacta aggctcatct ggtattgcag ttctgcggta
    13441 ggaggcgtca gcgcctgcgc cggaatgtgt tgcgttgagg tcatcgtcag agcaaccaca
    13501 ctggttgcat cccgtccacg ttgcatgact tttttaagtt cactggcaaa atcgatatcg
    13561 cgcgcctgat aaccaagggt atcggcattg gcgatgtttg ctgccagcac ttcctgacgc
    13621 tgggcgcgca gattgagcgc ctcttgttga aaacgtaagg cggcgtcgag cttatcgagc
    13681 atatctcctc cgcaggtatc aaaattctgc catcagctta aatgccttta cgcgcgcgtt
    13741 atcggcggaa taaacgcaaa atgggtcgct atttatgccg ttgatgacca ttggggacgg
    13801 gtacaattca cgttgtagaa atagctgggg ggcgaaaatg ctggcaataa aacgtagcgt
    13861 ggcgatcatc gcgatactgt tcagtccgtt aagtgcggcg agcaatctca catcgcaatt
    13921 gcacaccttt tttagcgccc agctcgtggg ggtaagtgat gaggttcgtg tttctattcg
    13981 tacagcaccc aatctactac cgccgtgcga gcagccattg ctttcgatga gcaataattc
    14041 ccgcctgtgg ggcaatgtga atgtgttggc acgctgcggt aacgacaaac gatatttaca
    14101 ggttaatgtg caggccacag gaaattatgt ggttgccgcg atgcccattg tgcggggagg
    14161 aaagctggaa gctggcaatg tcaaactaaa acgcggacga ctggataccc tgccaccgcg
    14221 tacggtgctg gatatcaatc aacttgttga tgccattagc ctacgcgatc tatcacccga
    14281 tcaaccaatc caattaaccc attttcgcca ggcatggcgg gtaaaagcgg gacaacgcgt
    14341 caacgtgatc gccagcggtg acgggtttag cgccaacgca gaaggtcagg cgctgaacaa
    14401 tgcagccgtc gcacagaatg cgcggatgcg catggtatcg ggactggtgg tcagcggcgt
    14461 tgttgatgca gatgggaata ttcttataaa cctgtaagct gttaaagatt acccgtccct
    14521 tgccgataaa taagcaacac atgataaaag cgccctcaat gaggaataaa ccatgagtat
    14581 tgatcgcact tcgcctctga atcccgtaag caccgttcaa ccgcgcgaaa ccactgacgc
    14641 gccggtaacg aacacccggg cggcaaaaac aaccgact
//
LOCUS       DAZPEB010000100        14649 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZPEB010000100 DAZPEB010000000
VERSION     DAZPEB010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14649
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.101"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..209)
                     /locus_tag="ACHURJ_002557"
     CDS             complement(<1..209)
                     /locus_tag="ACHURJ_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR1281453.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACHURJ_002558"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACHURJ_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFR1281454.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACHURJ_002559"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACHURJ_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFR1281455.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACHURJ_002560"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACHURJ_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFR1281456.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACHURJ_002561"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACHURJ_002561"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFR1281457.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACHURJ_002562"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACHURJ_002562"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFR1281458.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACHURJ_002563"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACHURJ_002563"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFR1281459.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYRGGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="ACHURJ_002564"
     CDS             complement(4598..5320)
                     /locus_tag="ACHURJ_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1281460.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACHURJ_002565"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACHURJ_002565"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR1281461.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="ACHURJ_002566"
     CDS             6520..6738
                     /locus_tag="ACHURJ_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFR1281462.1"
                     /translation="MQDLSLEARLAELESRLAFQESTIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACHURJ_002567"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACHURJ_002567"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFR1281463.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /locus_tag="ACHURJ_002568"
     CDS             complement(7472..7672)
                     /locus_tag="ACHURJ_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFR1281464.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7682..9493)
                     /gene="kefB"
                     /locus_tag="ACHURJ_002569"
     CDS             complement(7682..9493)
                     /gene="kefB"
                     /locus_tag="ACHURJ_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFR1281465.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEE
                     DEKPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYY
                     GDATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHE
                     LLQAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHA
                     DTVQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="ACHURJ_002570"
     CDS             complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="ACHURJ_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFR1281466.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELANHARAYGDWLANPLSPGGR"
     gene            10175..12088
                     /locus_tag="ACHURJ_002571"
     CDS             10175..12088
                     /locus_tag="ACHURJ_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1281467.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALSQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12088..13110
                     /locus_tag="ACHURJ_002572"
     CDS             12088..13110
                     /locus_tag="ACHURJ_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFR1281468.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQARHKPRLVIFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACTYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13104..13322
                     /locus_tag="ACHURJ_002573"
     CDS             13104..13322
                     /locus_tag="ACHURJ_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFR1281469.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13376..14245
                     /locus_tag="ACHURJ_002574"
     CDS             13376..14245
                     /locus_tag="ACHURJ_002574"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFR1281470.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14300..14563)
                     /locus_tag="ACHURJ_002575"
                     /pseudo
     CDS             complement(14300..14563)
                     /locus_tag="ACHURJ_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
BASE COUNT         3615 a         4001 c         3603 g         3430 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgattgtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt cagaccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcgttccagt taatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccgga ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccatgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt cacctatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcgaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagaccca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcacttgcc
     4441 ggtgaggtca attggttagc gttatagacc cctccccgat agaaaaagac gctgcttaac
     4501 tcatggcctt ctactatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctggaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggtgct ggtttcacat ccagcagctc tacgtcaaac
     5581 accagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gctgctttgc tgtcagcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agcagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcaaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaagcaccat tgaagaactg
     6601 aacgtcacgg tgaccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtgacc atgagccagt tcttcttcgg tcgcttcacg
     6961 aatcgccaca acctcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccactacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggcc cctggtcggt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc accaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gcgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacgca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgtg
     7621 cctgacaggc cgggcatttt gcgcccgcaa taaaacgttt tcggattgcc atctttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtaca gtatcggcat
     7801 gcattgggat gagctctcgc agcatccgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgcgcctg atgcggatgc atcccaagcg tgaccaaagt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaat agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggataa ccatttatcc accagcttca tcagcaacgg cgtggtcatc atggaaagcg
     8401 tcaccgtcac cgtcaccagc aacagcgcca tctggtcgcc ctggaataaa cgttgtgaag
     8461 aagcggtaga aaagaggaca aaggcaaact caccgccctg actcaacacg ccagcaaact
     8521 gcatccgctc tgagctacgc acgccataca atcgcgccag cagatacagc acgagaattt
     8581 tcaccgccac cagcacaacc acgcttatca ctacccacaa cagatgggta taaagcaccc
     8641 cgaggttgag cgacatgccg acagagataa agaacaaacc gagcagcaag cctttgaagg
     8701 gatcgatagc cgtttccagt tcatggcgat attcactttc cgccagcagc acgcccgcaa
     8761 taaatgtacc gagcgccatc gacagcccca gcgcatccat aaacaatgcg gaacccaaca
     8821 ccagcagcag cgtcgcggcg gtgaacactt cccgcacgcc agaagctgca ataaagcgga
     8881 ataccggacg cagtaaatag cgcccaccaa tcagcatgcc gacaaacgcc agcaccttca
     8941 tgccgatctt catccagtcg aaatgttcgt ctgccgaccc cgccaacaac ggcactaacg
     9001 ccagtgctgg gatcactgcc agatcctgaa acagcagaac cgaaaacccg agctggccgg
     9061 attcgctgcg attcatccct ttctcacgca tcaattgcaa cgccattgca gttgaagaca
     9121 tcgcaaggcc aatgccaccg accaccgccg cctgccaggc gaaatccgtc agcatcaata
     9181 atcccgccag caacgccgcg cttaacagca cctgtgccgc gcctacgcca aaaatcgaac
     9241 gccgcagttg ccaaagtttg gagggattca actcaaggcc gatgataaac atcaggaata
     9301 ccacgccgag ttccgaaaag tggaggatct catcgacgtc gctaataaac cccagccccc
     9361 acgggccaat tgcaatccct gccagcaaat atcccaacac agcgccaatc cccagccgcg
     9421 atgccaacgg caccgcagcc accgccgcga agagaaacag cactcctgcg agtaaaaaat
     9481 cggaaccttc catcagcggc ctcctggaga cagcggattt gccagccagt caccgtaggc
     9541 tctggcgtgg ttcgccagct cctttgcgct ttgccgtctc gcccagtaaa taatgatggg
     9601 acttaaccaa tgcatccggc acatgcccgc cgccagttca aaggggcgca gcacatcgct
     9661 catcgggtag cgattcagcg cgtcataacg gtaagcactt tccggctcgc cggtggtaat
     9721 cacgttacgc cagtactttc ccgccagttg gtttcctccc ggcccgctgg caaaaccacg
     9781 acttaatacc cggtccagcc actctttcag tagcgccggg cagctatagg tataaagagg
     9841 atgctgaaag acaatcacct cgtgctcgcg cagtaatgcc tgctcacggg ggatatcaat
     9901 aaaaaaatcg ggatagtgcg cgtaaaggtc gtgcacggta acattgctga gctgcgtggc
     9961 cggtttaagc agtacccggt ttgccaccga gtcctgagat tccggatggg catacagcag
    10021 caaaactttc gctggctgag acatcatccc cctcccggta tggtttttgt gtatagtcgc
    10081 tgttttgggc taccattgcg cccggtgcgg cagctcgccc atacattaca ttatcataat
    10141 gataagttaa catagtctga acatacggcg ccttatgatt gttttctcct cgttacaaat
    10201 tcgtcgcggc gtgcgcgtcc tgctggataa tgccaccgcc accatcaacc ctgggcagaa
    10261 agtcggcctg gtgggtaaaa acggctgtgg taaatctacc ctgctggcat tgctgaaaaa
    10321 tgaaatcagc gccgacggcg gcagctacac ctttccggga agctggcaac tggcgtgggt
    10381 gaatcaggaa acgccggcgt tatcgcaagc ggcgctggaa tatgtcattg acggcgaccg
    10441 tgaatatcgt caactggaag cgcagctgca cgacgccaac gaacgtaacg acgggcacgc
    10501 cattgcgacc attcatggca agctggatgc tattgacgca tggagtattc gctcccgtgc
    10561 cgccagcctg ctgcacggcc tcggtttcag caatgaacaa ctggagcgcc cggtaagtga
    10621 tttctccggt ggctggcgta tgcgtcttaa ccttgcccag gcgctgattt gccgttcaga
    10681 cttgctgctg ctcgacgaac cgactaacca cctcgatctc gatgccgtta tctggctgga
    10741 aaaatggttg aagagctatc aaggcacgct gatcctgatc tctcacgacc gcgacttcct
    10801 cgatccgatc gttgataaaa ttattcatat cgaacaacaa agcatgttcg agtacaccgg
    10861 caactacagt tcgtttgaag tacagcgcgc cacccgtctg gcgcaacaac aagcgatgta
    10921 tgaaagccag caggaacgcg tggcacatct gcaaagttat atcgaccgtt tccgtgccaa
    10981 agccaccaaa gcgaagcaag cccagagccg cattaagatg cttgagcgta tggagctgat
    11041 tgcccccgcg cacgtcgaca acccgttccg ctttagcttc cgcgcgccgg aaagcctgcc
    11101 aaatccgtta ctgaagatgg aaaaagtcag cgcgggctat ggcgatcgca ttattctcga
    11161 ctcgattaaa ctgaacctgg tgcccggctc gcgcattggt ctgttaggcc gcaacggcgc
    11221 gggtaaatcg acattaatca aactgttagc cggtgaactt gcgccagtca gcggtgaaat
    11281 tggtctggcg aaagggatca agctcggcta cttcgcccag catcaacttg aatacctgcg
    11341 cgccgacgaa tcacctattc aacatctggc acgtttagcg ccgcaggagc tggaacaaaa
    11401 actgcgtgac tacctcggcg gctttggttt ccagggcgat aaagtaaccg aagaaacgcg
    11461 ccgcttctcc ggtggggaaa aagcccgcct ggtgctggca ttaattgtct ggcagcgtcc
    11521 gaatctgctg ctgctcgacg aaccgactaa ccaccttgac ctcgacatgc gtcaggcact
    11581 caccgaagca ttaatcgagt ttgaaggcgc gctggttgtc gtttcgcacg accgtcattt
    11641 gctgcgttcc accactgacg atctctacct ggttcacgat cgtaaagtcg aaccgttcga
    11701 cggcgatctg gaagattatc aacagtggtt gagcgacgta caaaagcagg aaaaccagac
    11761 cgacgaagcg ccaaaagaga acgcgaacag cgcccaggca cgtaaagatc agaagcgtcg
    11821 ggaagctgag ctgcgtgcgc aaacccagcc gctgcgtaaa gagattgccc gtctggaaaa
    11881 agagatggag aagctgaacg cgcaactggc gcaggcggaa gagaaactcg gcgacagcga
    11941 actgtatgac cagagccgta aagcggagtt gaccgcctgc ctgcaacagc aagccagcgc
    12001 caaatccggc ctggaagagt gcgaaatggc gtggctggaa gcccaggagc agcttgagca
    12061 gatgctgctg gaaggccaaa gcaactgatg gcgcagataa cgacgaccga tgccaatgaa
    12121 ttcagcagca gtgctgaatt cacccctatg cgcggcttta gcaattgtca tctacaaact
    12181 atgctgccgc gtttgtttcg tcgtcaggtg aaattcaccc cgtactggca gcggctggag
    12241 ttgcccgacg gcgattttgt cgatctcgcg tggagtgaag cccctgcaca ggctcggcat
    12301 aaaccgcgtt tagtgatatt tcacgggctg gaaggcagtc tcaatagccc ttacgcccac
    12361 ggtctggttg aggcggcgca aaagcgcggc tggctgggcg tggtgatgca ttttcgcgga
    12421 tgcagcggtg aaccaaaccg tatgcaccgc atttaccatt cgggcgaaac cgaagacgcc
    12481 agttggtttt tacgctggct gcaacgcgaa tttggtcatg cgccaacggc tgccgtcggc
    12541 tattcgctcg gcggtaatat gctggcctgt ttgctggcaa aggaaggcaa tgatctcccg
    12601 gttgatgcgg cggtgattgt ctctgcgccg tttatgctgg aagcctgtac ctatcatatg
    12661 gaaaagggct tttcccgcgt ttatcagcgt tacttgctga acctgttaaa agccaatgcc
    12721 gcgcgcaagc tggcagccta ccccggaacg ctgccgatta atctcgcgca gttaaaatcg
    12781 gtacgtcgca tccgtgaatt tgacgatcta atcaccgcca gaattcacgg ctacgccgac
    12841 gctatcgact attatcgtca gtgtagcgcc atgccgatgc tgaaccggat cgccaaaccg
    12901 acgctgatta ttcacgccaa agacgatccg tttatggatc atcaggtgat cccgaaaccg
    12961 gaaagtctcc cctcgcaggt ggagtatcaa ctgactgaac atggcggtca tgttggcttt
    13021 attggcggta cattacttca tccgcaaatg tggctggagt cacgcattcc tgactggtta
    13081 acaacgtatc tggaggcgaa atcatgttga ttccgtggca agacctctcc cccgaaacgc
    13141 tggaaaattt aattgaaagc tttgtgttac gtgaaggcac cgattatggt gaacatgagc
    13201 gtacacttga acagaaagtc gccgacgtca aacgccagct acagtgcgga gaagcggtgc
    13261 tggtatggtc ggagctgcac gaaacggtca atattatgcc gcgcagtcag tttcgcgaat
    13321 aacctgcaac cgtcggcgga cgagataata atgatagcta agtcatggag ttactatgtc
    13381 tgccaaacat ccggtcattg cggtaacagg atccagcggc gcggggacca ccaccaccag
    13441 cctcgcgttt cgtaaaatat tcgcgcagtt aaatctgcat gcagctgagg tggaaggcga
    13501 cagttttcac cgttacaccc gcccggaaat ggacatggcg atccgcaaag cgcgcgacgc
    13561 cgggcggcat atcagctact tcggccccga agcgaacgac ttcggcctgc tggaacaaac
    13621 cttcattgaa tacggtcaga gcggcaaagg gaaatctcgc aaatatctgc atacctacga
    13681 cgaagccgta ccgtggaatc aggtaccggg aacattcacc ccctggcaac ctttaccaga
    13741 acccactgat gtactgtttt atgaaggttt acacggcggc gtagtcacgc cacagcataa
    13801 cgttgcgcag catgtggact tactggtcgg cgtagtgcct atcgttaacc ttgagtggat
    13861 tcaaaaactg atccgcgaca ccagcgagcg cgggcactca cgagaagcag tgatggactc
    13921 agtagtgcgt tcaatggaag actatatcaa ctacatcaca ccgcagtttt cccgcaccca
    13981 tcttaacttc cagcgcgttc ccaccgtcga cacttcaaac ccgttcgcgg caaaaggtat
    14041 cccgtcgctg gatgaaagct ttgtggtgat ccattttcgt aatctggaag ggatcgattt
    14101 cccctggctg ctggcgatgt tgcaaggctc attcatttcc cacatcaata cgttagtggt
    14161 accgggcggc aaaatgggtc tggcaatgga attaattatg ctgccgctgg tgcaacgatt
    14221 gatggaagga aagaaaatcg agtaactctg ctattacgcc ggataaaata ctatccggct
    14281 tcacaacggg atagttaaat cacgcggcaa ccacttcata cgagtgagta atattcaccg
    14341 ctttttccag catcaacgcc actgaacaat atttctcggc agagagatca accgcacgcg
    14401 caaccgctgc gtctttcagg tcgcgaccgg tgacgataaa atgcagatta atgtgcgtaa
    14461 acaggcgtgg tgcctcttcg cggcgttcag aggtcaattt tacttcacaa tcgaccacat
    14521 cctgacgccc tttttgcagg atcgaaacca catcgatggc actgccccgt tactttattc
    14581 gtacccctta taatggggtg ttagccagcc agacccggca tgattactgc ccccagtcgt
    14641 ccatgatcc
//
LOCUS       DAZPEB010000101        14389 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZPEB010000101 DAZPEB010000000
VERSION     DAZPEB010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14389
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.102"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            158..1096
                     /gene="tdcA"
                     /locus_tag="ACHURJ_002576"
     CDS             158..1096
                     /gene="tdcA"
                     /locus_tag="ACHURJ_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFR1281471.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRLLIEVG"
     gene            1195..2184
                     /gene="tdcB"
                     /locus_tag="ACHURJ_002577"
     CDS             1195..2184
                     /gene="tdcB"
                     /locus_tag="ACHURJ_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFR1281472.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            2206..3537
                     /gene="tdcC"
                     /locus_tag="ACHURJ_002578"
     CDS             2206..3537
                     /gene="tdcC"
                     /locus_tag="ACHURJ_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFR1281473.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            3563..4771
                     /gene="tdcD"
                     /locus_tag="ACHURJ_002579"
     CDS             3563..4771
                     /gene="tdcD"
                     /locus_tag="ACHURJ_002579"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFR1281474.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSCGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            4805..7099
                     /gene="tdcE"
                     /locus_tag="ACHURJ_002580"
     CDS             4805..7099
                     /gene="tdcE"
                     /locus_tag="ACHURJ_002580"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFR1281475.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFEAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNIMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            7113..7502
                     /locus_tag="ACHURJ_002581"
     CDS             7113..7502
                     /locus_tag="ACHURJ_002581"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFR1281476.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            7574..8938
                     /gene="tdcG"
                     /locus_tag="ACHURJ_002582"
     CDS             7574..8938
                     /gene="tdcG"
                     /locus_tag="ACHURJ_002582"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFR1281477.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGALSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            9212..10544
                     /locus_tag="ACHURJ_002583"
                     /pseudo
     CDS             9212..10544
                     /locus_tag="ACHURJ_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10572..11882
                     /gene="cyuA"
                     /locus_tag="ACHURJ_002584"
     CDS             10572..11882
                     /gene="cyuA"
                     /locus_tag="ACHURJ_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFR1281478.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNS
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(12016..12180)
                     /gene="yhaL"
                     /locus_tag="ACHURJ_002585"
     CDS             complement(12016..12180)
                     /gene="yhaL"
                     /locus_tag="ACHURJ_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFR1281479.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(12203..12904)
                     /locus_tag="ACHURJ_002586"
     CDS             complement(12203..12904)
                     /locus_tag="ACHURJ_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFR1281480.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            13009..13905
                     /gene="yhaJ"
                     /locus_tag="ACHURJ_002587"
     CDS             13009..13905
                     /gene="yhaJ"
                     /locus_tag="ACHURJ_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFR1281481.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFSGK"
     gene            complement(13956..14312)
                     /locus_tag="ACHURJ_002588"
     CDS             complement(13956..14312)
                     /locus_tag="ACHURJ_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFR1281482.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGVWALLYLVPIIGWLVLFVFACLEG
                     NSGSNRYGNDPKFGSN"
BASE COUNT         3459 a         3358 c         3837 g         3735 t
ORIGIN      
        1 tttttatgtt ttaacttgtt gatatttaaa gatatttaat tgtaataacg ataccctgga
       61 aagtaatgaa agttaatttg tgagtggtcg cacatatcct gttcatttca ttttgataca
      121 cttcatgccg tcaatgaggt aattaacgta ggtcgttatg agcactattc ttcttccgaa
      181 aacgcagcac ctggtagtct ttcaggaagt cattagaagt ggctctatcg gctcggctgc
      241 aaaagaatta gggttaactc aaccggccgt cagtaaaatc attaacgata ttgaagatta
      301 ttttggtgtg gaattagtgg tgcggaaaaa taccggtgta acattaacac ctgccggtca
      361 attgttactc tcccgttccg aatccattac ccgtgaaatg aaaaatatgg ttaatgagat
      421 aagcggtatg tcttctgagg cggtggtgga agtctcattt ggttttcctt cattgattgg
      481 ttttactttt atgtcaggga tgatcaacaa gttcaaagag gtgttcccga aagcgcaggt
      541 ttctatgtat gaagcgcaac tgtcttcgtt cttaccggca atccgcgacg gtcgactgga
      601 ttttgcgatt ggtacgttaa gtgcagaaat gaagcttcag gatttacatg ttgagccgct
      661 gttcgagtcc gagtttgtgc tggtagccag taagtcccga acatgcaccg gcaccaccac
      721 gctggagtcg ttgaagaacg aacagtgggt gttgccacaa accaatatgg ggtactacag
      781 cgagcttctt actacgttac aaagaaatgg catcagtatt gaaaacatcg ttaaaaccga
      841 ctcagtcgtg acaatttata atcttgttct caatgctgat ttcttaactg taattccttg
      901 tgatatgacg tcaccttttg gttcaaatca atttattact attccagttg aagaaacatt
      961 acctgtggca caatatgccg cggtatggtc gaaaaattat cgtattaaaa aagcagcatc
     1021 ggttttggtg gaattagcca aagagtattc atcttataat gggtgtagac gaaggctatt
     1081 aatagaagtt ggttagttat ttgtttttat ttaaacataa ataatccacc tgtctgtttt
     1141 gcaggtgtcg gttacggtta tctacatatt taattcaggc gaagaggttt tataatgcat
     1201 attacatacg atctgccggt tgctattgat gatattattg aagcgaaaca acgactggct
     1261 gggcgaattt ataaaacagg catgcctcgc tccaactatt ttagtgaacg ttgcaaaggt
     1321 gaaatattcc tgaagtttga aaatatgcag cgtacgggtt catttaaaat tcgtggcgca
     1381 tttaataaat taagttcact gaccgatgcg gaaaaacgca aaggcgtggt ggcctgttct
     1441 gcgggcaacc atgcgcaagg ggtttcgctc tcctgcgcga tgctgggtat cgacggtaaa
     1501 gtggtgatgc caaaaggtgc gccaaaatcc aaagtagcgg caacgtgcga ctactccgca
     1561 gaagtcgttc tgcatggtga taacttcaac gacactatcg ctaaagtgag cgaaattgtc
     1621 gaaatggaag gtcgtatttt tatcccacct tacgatgatc cgaaagtgat tgctggccag
     1681 ggaacgattg gtctggaaat tatggaagat ctctatgatg tcgataacgt gattgtgcca
     1741 attggtggtg gtggtttaat tgctggtatt gcggtggcaa ttaaatctat taacccgacc
     1801 attcgtgtta ttggcgtaca gtcagaaaac gttcacggca tggcggcttc tttccactcc
     1861 ggagaaataa ccacgcaccg aactaccggc accctggcgg atggttgtga tgtctcccgc
     1921 ccgggtaatt taacttacga aatcgttcgt gaattagtcg atgacatcgt gctggtcagc
     1981 gaagacgaaa tcagaaacag tatgattgcc ttaattcagc gcaataaagt cgtcaccgaa
     2041 ggcgcaggtg ctctggcatg tgctgcatta ttaagcggta aattagacca atatattcaa
     2101 aacagaaaaa ccgtcagtat tatttccggc ggcaatatcg atctttctcg cgtctctcaa
     2161 atcaccggtt tcgttgacgc ttaattaatt cgttgaggat aggatatgag tacttcagat
     2221 agcattgtat ccagccagac aaaacaatcg tcctggcgta aatcagatac cacatggacg
     2281 ttaggcttgt ttggtacggc aatcggcgcc ggggtgctgt tcttccctat ccgcgcaggt
     2341 tttggcggac tgatcccgat tcttctgatg ttggtattgg cataccccat cgcattttat
     2401 tgccaccggg cgctggcgcg tctgtgtctt tctggctcta acccttccgg caacattacg
     2461 gaaacggtgg aagagcattt tggtaaaact ggcggcgtgg ttatcacgtt cctgtacttc
     2521 ttcgcgattt gcccactgct gtggatttat ggcgttacta ttaccaatac ctttatgacg
     2581 ttctgggaaa accagctcgg atttgcaccg ctgaatcgcg gctttgtggc gctgttcctg
     2641 ttgctgctga tggctttcgt catctggttt ggtaaggatc tgatggttaa agtgatgagc
     2701 tacctggtat ggccgtttat cgccagcctg gtgctgattt ctctgtcgct gatcccttac
     2761 tggaactctg cagttatcga ccaggttgac ctcggttcgc tgtcgttaac cggtcatgac
     2821 ggtatcctga tcactgtctg gctggggatt tccatcatgg ttttctcctt taacttctcg
     2881 ccaatcgtct cttccttcgt ggtttctaaa cgtgaagagt atgagaaaga cttcggtcgc
     2941 gacttcaccg aacgtaaatg ttcccaaatc atttctcgtg ccagcatgct gatggttgca
     3001 gtggtgatgt tctttgcctt tagctgcctg tttacgctgt ctccggccaa catggcggaa
     3061 gccaaagcgc agaatattcc agtgctttct tatctggcta accactttgc gtccatgacc
     3121 ggtaccaaaa caacgttcgc gattacactg gaatatgcgg cttccatcat cgcactcgtg
     3181 gctatcttca aatctttctt cggtcactat ctggggacgc tggaaggctt gaatggtctg
     3241 attctgaagt tcggttataa aggcgacaaa actaaagtgt cgctgggtaa actgaacact
     3301 atcagcatga tcttcatcat gggctccacc tgggttgttg cctacgccaa cccgaacatc
     3361 ctcgacctta ttgaagccat gggcgcaccg attatcgcat ccctgctgtg cctgttgccg
     3421 atgtatgcca tccgtaaagc accgtctctg gcgaaatacc gtggtcgtct ggataacgtg
     3481 tttgttaccg tgattggtct gctgaccatc ctgaacatcg tatacaaact gttttaatcc
     3541 gtaactcagg atgagaaaag agatgaatga atttccggtt gttttggtta ttaactgtgg
     3601 ttcgtcttcg attaagtttt ccgtactcga tgccagcgac tgtgaagtat taatgtcagg
     3661 tattgccgac ggtattaact cagaaaatgc attcttatcc gtaaatgggg gagagccagc
     3721 accgctggct caccacagct acgaaggtgc attgaaggca attgcatttg aactggaaaa
     3781 acggaattta aatgacagtg tggccttaat tggccaccgc atcgctcacg gcggcagtat
     3841 ttttaccgag tccgccatta ttaccgatga ggtcattgat aatatccgtc gcgtttctcc
     3901 actggcaccc ctgcataatt acgccaattt aagtggtatt gaatcggcgc agcaattatt
     3961 tccgggcgta actcaggtgg cggtatttga taccagtttc caccagacga tggctccgga
     4021 agcttattta tacggcctgc cgtggaaata ttatgaagag ttaggtgtac gccgttatgg
     4081 tttccacggc acgtcgcacc gctatgtttc ccagcgcgca cattctttgc tgaatctagc
     4141 ggaagatgac tccggcctgg ttgtggcgca tcttggcaat ggcgcgtcaa tctgcgcagt
     4201 tcgcaacggt cagagtgttg acacctcaat gggaatgacg ccgctggaag gcttgatgat
     4261 gggtacccgc agtggcgatg tcgattttgg tgcgatgtcc tgggtcgcca gccaaaccaa
     4321 ccagagcttg ggtgacctgg aacgcgtagt gaataaagag tcgggattat taggtatttc
     4381 cggtctttct tcggatttac gtgttctgga aaaagcctgg catgaaggtc acgaacgcgc
     4441 gcaactggca attaaaacct ttgttcaccg aattgcccgt catattgccg gacacgcagc
     4501 ttcattacgt cgcctggatg gaattatatt caccggcgga ataggagaga attcaagctt
     4561 aattcgtcgt ctggtcatgg aacatttggc tgtattaggc gtagagattg atacagaaat
     4621 gaataatcgc tctaactcct gtggtgagcg aattgtttcc agtgaaaatg cgcgtgtcat
     4681 ttgcgctgtt attccgacta acgaagaaaa aatgattgct ttggatgcca ttcatttagg
     4741 caaagttaac gcgcccgcag aatttgcata atttagttga agtattgtag agagattatt
     4801 tttcatgaag gtagatattg ataccagcga taagctgtac gccgacgcat ggcttggctt
     4861 taaaggtacg gactggaaaa acgaaattaa tgtccgcgat tttattcaac ataactatac
     4921 accgtatgaa ggcgatgaat ctttcctcgc cgaagcgacg cctgccacca cggaattgtg
     4981 ggaaaaagta atggaaggta tccgtatcga aaacgcaacc cacgcgccgg ttgatttcga
     5041 taccaatatt gccaccacaa ttaccgctca tgatgcggga tatattaacc agccgctgga
     5101 aaaaattgtt ggcctgcaaa cggatgcgcc gttgaaacgt gcgctacacc cgttcggtgg
     5161 cattaatatg attaaaagtt cattccacgc ctatggccgt gaaatggaca gtgaatttga
     5221 atatctgttt accgatctgc gtaaaaccca taaccagggc gtatttgatg tttactcacc
     5281 ggatatgctg cgctgccgta aatctggcgt gctgaccggt ttaccagatg gctatggccg
     5341 tgggcgcatt atcggtgact atcgccgcgt agcgctgtat ggcatcagtt atctggtacg
     5401 tgaacgcgaa ctgcaatttg ccgatctcca gtctcgtctg gaaaaaggcg aggatctgga
     5461 agccaccatc cgtctgcgtg aggagctggc agagcatcgt catgcgctgt tgcagattca
     5521 ggaaatggcg gcgaaatatg gctttgatat ctctcgcccg gcgcagaatg cgcaagaagc
     5581 ggtgcagtgg ctctacttcg cttatctggc ggcagtgaaa tcgcaaaatg gcggcgcgat
     5641 gtcgctgggc cgcacggcat cgttcctcga tatctacatt gagcgcgact ttgaagctgg
     5701 cgtactcaat gagcagcagg cacaggaact gatcgatcac ttcatcatga agatccgtat
     5761 ggtacgcttc ctgcgtacac cggaatttga ttcgctgttc tccggcgacc caatctgggc
     5821 gacggaagtg atcggcggga tggggctgga cggtcgtacg ctggtgacca aaaactcctt
     5881 ccgctatttg cacaccctgc acactatggg gccggcaccg gaacctaacc tgaccattct
     5941 ttggtcggaa gaattaccga ttgccttcaa aaaatatgcc gcgcaggtgt cgatcgtcac
     6001 ctcttccttg cagtatgaaa atgacgatct gatgcgtact gacttcaaca gcgacgatta
     6061 cgcgattgcc tgctgcgtca gcccaatggt gattggtaag caaatgcagt tctttggtgc
     6121 acgcgctaac ctggcgaaaa cgctgctcta cgcaattaac ggcggggtgg acgagaagct
     6181 gaagattcag gtcgggccga aaacagcacc gctgatggac gacgtgctgg attacgacaa
     6241 agtgatggac agcctcgatc acttcatgga ctggctggcg gtgcagtaca tcagcgcgct
     6301 gaatatcatt cactacatgc acgacaagta cagctacgaa gcttcgctga tggcgctgca
     6361 cgatcgtgat gtctatcgca ctatggcatg cggcatcgcg ggcctgtcgg tggcgacgga
     6421 ctccctgtct gccatcaaat atgcccgcgt gaaaccaatc cgtgacgaaa acggcctggc
     6481 ggtggacttt gaaatcgacg gtgaatatcc gcagtacggc aacaacgacg agcgcgtaga
     6541 cagcattgcc tgcgacctgg ttgaacgctt tatgaagaaa attaaagcgc tgccaaccta
     6601 tcgcaacgcc gtccctaccc agtcgattct gactatcact tctaacgtgg tgtacggcca
     6661 gaaaaccggt aatacgccgg acggtcgtcg cgccggaaca ccgttcgcgc cgggcgctaa
     6721 cccgatgcat ggtcgtgacc gcaaaggtgc cgtggcctca ttgacgtcgg tggcgaaact
     6781 gccgttcacc tacgccaaag atgggatctc gtacaccttc tcaatcgttc ctgcggcgct
     6841 gggcaaagaa gatccagtac gtaaaaccaa ccttgtcggc ctgctggatg ggtatttcca
     6901 ccacgaagcg gatgtcgaag gcggtcaaca cctcaacgtc aacataatga atcgggaaat
     6961 gctgctggat gccatcgagc acccggaaaa atatcctaac ctgacaatcc gtgtctctgg
     7021 ctacgccgtg cgcttcaacg cactgacccg tgaacagcaa caggatgtta tttcacgtac
     7081 ttttacccag gcgctctgac gccggaggat gtatgaaaaa gattatcgaa acgcaacgtg
     7141 ccccaggcgc aatcggccct tatgttcagg gcgttgattt aggcagcatg gtcttcacct
     7201 ccgggcaaat tccggtttgc ccgcagaccg gtgagatccc ggctgatgtg caagatcagg
     7261 cgcgtttaag cctcgaaaac gtcaaagcga tcgtggttgc tgccgggctg agcgtgggcg
     7321 atatcatcaa gatgaccgtg ttcatcaccg atctgaatga ttttgccacc atcaatgaag
     7381 tctataagca gttcttcgat gagcatcagg cgacctaccc gacccggagc tgtgtgcagg
     7441 ttgcgcgttt gccgaaagat gtgaagctgg aaattgaagc tatcgcagta cgtagtgcgt
     7501 aaagcctcgt gagcgggacg gtcgcaaggt cgttccgctc cacttcactg aacggcaatc
     7561 cgagggtgtg gatatgatta gtgcattcga tattttcaaa attgggattg gtccctccag
     7621 ttcgcatacc gtggggccaa tgaatgccgg aaaaagtttt attgatcggc tggaaagtag
     7681 cggcttatta accgcgacga gccatattgt ggtcgatctg tatggggcgt tgtcactgac
     7741 gggcaaaggc catgccacgg atgtcgccat tatcatggga ctggcaggaa acagtccgca
     7801 ggatgttgtc attgatgaga tccctgcatt tatagagtta gtaacgcgca gcgggcggct
     7861 gccagtggca tctggtgcgc atattgttga ttttcctgta gcaaagaaca ttatcttcca
     7921 tcccgaaatg ttgcctcgcc atgagaacgg aatgcggatc actgcctgga agggacagga
     7981 agcgctatta agtaaaacct attactctgt cggcggcggg tttattgtcg aagaagaaca
     8041 cttcggcctg tcgcacgatg tcgaaacgtc cgtaccttac gatttccact cagcaggtga
     8101 actgctgaaa atgtgtgatt acaacggcct gtctatatct ggcctgatga tgcacaacga
     8161 gctggcgctg cgcagcaaag cggaaattga cgcagggttt gcccgtatct ggcaagtgat
     8221 gcatgacggt attgaacgcg ggatgaacac tgaaggcgtg ctgcctggtc cgctcaatgt
     8281 gccgcgtcgt gccgtagcgc tgcgtcgtca gctggtttct agcgataaca tctctaacga
     8341 tccgatgaat gtcatcgact ggatcaacat gtacgcgctg gcggttagtg aagaaaacgc
     8401 agctggcggg cgcgtggtaa cggcaccgac taacggtgcg tgcggcatta ttccggcagt
     8461 gctggcttat tacgataagt tccgtcgtcc ggtaaacgag cggtcaattg cccgctattt
     8521 tctggcagcg ggggctattg gcgcgctgta taaaatgaac gcctccatct ctggcgcgga
     8581 ggtcggctgt cagggggaga ttggcgtggc ctgttcaatg gcggcggcag ggttaactga
     8641 actactgggc ggcagtccgg cgcaggtatg caatgcggcg gaaatcgcga tggagcataa
     8701 ccttgggctg acctgcgatc cggttgccgg acaggtacaa atcccgtgca ttgaacgtaa
     8761 tgccattaat gccgtgaaag cagtaaacgc cgcgcggatg gcgatgcgcc gcacctcggc
     8821 accgcgtgtt tcactcgata aagtgatcga gacgatgtat gaaaccggca aagatatgaa
     8881 cgataaatac cgcgaaacat cacgcggagg actggccatt aaagtggtct gcggctgaca
     8941 ttgctgtcag ctttcatcct tgggtgcaca aatgtgcacc tttttttgtg atctgccgca
     9001 aatagagaat gaatattctc ttgcgattaa tttagtagaa aattcttatc catctccgtt
     9061 atttcaaagt cttgattgtg aagattttct ctgacctcgc gcgtatcatt tctttattct
     9121 aataactcgc ctgcaaaaac gtagaccgca tatcgccttg atgcggctcg gaaaaaaata
     9181 ataagcccta caaaatattg agcgagaaat tatggaaatt gcatcgaata aaggcgtcat
     9241 tgcagacgct tcgaccccgg cgggtcgtgc tggaatgagt gagagcgagt ggcgagaagc
     9301 gatcaaattc gacagtactg acaccggctg ggtgattatg agtatcggga tggcgattgg
     9361 cgcggggatt gtttttctcc cggtgcaggt cggtttgatg ggattgtggg tatttttgct
     9421 ctcatcggtg attggttacc cggcaatgta tctgtttcag cggttgttta ttaatacgct
     9481 ggcagaatca cacagaatgt aaagattacc cgagtgtcat tagcggttat ttaggtaaaa
     9541 actggggcat cctgttaggt gcgctctatt tcgtaatgct ggtgatttgg atgttcgtct
     9601 attccaccgc catcaccaac gatagtgctt cctatctgca taccttcggc gtgacggaag
     9661 gggtgctgtc agacagtccc ttttatggtc tggtactgat ttgcattctg gtggcgatct
     9721 cttcacgcgg cgagaaattg ttattcaaaa tttcgaccgg catggtgctg accaagctgc
     9781 tggtagtcgc tgcgctgggc gtgtcgatgg tgggaatgtg gcatctgtat aacgtcggtt
     9841 cgctaccgcc gctggggctg ctggtgaaaa acgccattat tacgctgccg tttaccctga
     9901 cgtcgattct gtttatccag acgttaagcc cgatggtgat ctcttatcgc tcgcgggaaa
     9961 aatcaattga agtggcgcgg cataaagcat tgcgggcaat gaatatcgcg tttggcattt
    10021 tatttgtcac cgtctttttc tacgccgtgt cgttcacgct ggcgatggga catgacgaag
    10081 cggtaaaagc ctacgagcag aatatttccg cgctggcgat tgccgcacag tttattagcg
    10141 gtgacggcgc agcgtgggtg aaagtggtca gcgtcattct caatatcttt gctgtcatga
    10201 ctgcattctt tggcgtctat ttaggctttc gcgaagcaac gcaagggatc gtaatgaaca
    10261 tcctgcgtcg caagatgcct gccgagaaga ttaacgaaaa tcttgttcag cgcggcatca
    10321 tgattttcgc cattttgctg gcctggagcg ccatcgtact gaacgcaccg gtgttgagct
    10381 tcacctctat ctgtagcccg attttcggca tggtagggtg cctgatcccg gcgtggctgg
    10441 tttacaaagt accggcattg cacaaataca aagggatgtc tctgtacctg attatcgtca
    10501 ctggtttgtt gctttgtgtt tctccgttcc tggcattttc ttgatttacc tgaaatttta
    10561 aggtttttaa tatgtttgat tcgactttaa atccgttatg gcagcgttac atcctcgccg
    10621 ttcaggagga agtaaaaccg gcgctgggat gtactgaacc gatttcactg gcgctggcgg
    10681 cggcggttgc tgcggcagaa ctggaaggtc cggttgaacg tgtagaagcc tgggtttcgc
    10741 caaatctgat gaagaacggt ctgggcgtca ccgttcccgg cacgggaatg gtggggctgc
    10801 cgattgcggc ggcgctgggg gcgttaggtg gaaatgccaa cgccgggctg gaagtgctga
    10861 aagacgcaac tgcgcaggca attgccgatg ccaaagcact gctggcggcg gggaaagtct
    10921 ccgttaagat ccaggaacct tgcaatgaaa tcctcttctc acgcgccaaa gtctggaacg
    10981 gtgagaagtg ggcgtgtgtc accatcgtcg gcgggcatac caacattgtg catattgaga
    11041 cgcacaatag tgtggtgttt acccagcagg cgtgtgtggc agagggcgag caagagtctc
    11101 cgctgacggt gctttccaga acgacgctgg ctgagatcct gaagttcgtc aatgaagtcc
    11161 cgtttgcggc gatccgcttt attctcgatt ccgcgaagct aaattgtgcg ttatcgcagg
    11221 aaggtttgag cggtaagtgg gggctgcata ttggcgcgac gctggaaaaa cagtgcgagc
    11281 gcggtttgct ggcgaaagat ctctcttcat ccattgtgat tcgtaccagc gcggcatccg
    11341 atgcgcgtat gggcggcgct acgcttccgg ctatgagtaa ctccggctcg ggtaaccagg
    11401 ggatcaccgc aacaatgcct gtggtggttg tagcagaaca cttcggagcg gatgatgaac
    11461 gactggcgcg tgcgctgatg ctttctcatt tgagcgcaat ttacatccat aaccagttac
    11521 cgcgtttgtc tgcactttgt gccgcaacga ccgcagcaat gggggccgcc gccgggatgg
    11581 catggctggt ggatgggcgt tatgaaacta tctcgatggc gatcagcagt atgatcggcg
    11641 atgtcagcgg catgatttgc gatggtgcgt cgaacagctg cgcgatgaag gtttcgacca
    11701 gtgcttcggc tgcgtggaaa gcggtgttaa tggcgctgga tgataccgcc gtgaccggca
    11761 atgaagggat cgtggcgcat gatgttgagc agtcgattgc caacctgtgt gcgttagcaa
    11821 gccattcgat gcagcaaacg gatcggcaga ttatcgagat tatggcgagc aaggccagat
    11881 aaacgccagg ttttgagtat tgtgccggat gcgatgctgg cgcaccttat ccggcctacg
    11941 ggttgtttaa ttcgttaatg ttgttggatt ttgtaggccg gataaggcgt tttcgccgca
    12001 tccggcaacc tgtctttacg cggtagcttc atcctcggct aaacgcgttt cagcatccgc
    12061 gacgatggct tccagctcgc tcaacatctc ttcatagcca aaatttttgg cggtgcgagc
    12121 aggttctttc gccaccacgg gcttcactgg ctggcgcttt ttggccgatt ttttactcat
    12181 catcatctcc ctgtaaaaac aactagacag gcaaatctat cagcaaagcg cgcagtgggg
    12241 aatcggcaac cagcgtaatg ttagcctcgt cacgaataaa cgccccatca ccgcaggtca
    12301 gcgccgcttt ctcttcatga tgcgtaagcg catgaaattt cccgtgaatc gattgcaaat
    12361 acgcgcgtgg cccatgcaac tggaaattcg cactttcgcc tttgtcgagc acgatatggt
    12421 gcagccacac ttgctggcgt aattgcaggc ttcccatcgt accctctggc gaggcgatta
    12481 attgctgctt gcccatatta agcgccagtt tttgaatcag cggattctct cgctgcgggc
    12541 aggcgtccag ccaaagctgc attcgtgtta acggtttgtc tttgctgaga ttgtgttcgc
    12601 tgtagctgac acccggctgg gtagagagca gcaacgcctc accggcgctg gcctgaacat
    12661 gattgccttc gctgtcgcga tactctgctt ctccatccag aatcacattt aaaatatcga
    12721 ctttgggata ggttcgcggc tgaaaggcgg cacctggggc cagcacttcc tggttaagca
    12781 cacgcaggga ggcatagcct aacaatttcg ggtcgaagta gtgtccaaag gaaaaagtat
    12841 accgggcctg caaccatccg tagtctgctt gtccacactg cctggcagtt cgggtagtaa
    12901 tcataattct tgacctctct ttacttcctt ttatggtaag gggctggacg ctacattgtt
    12961 agccagatat tctgcccggt atgttcaaat ttcctgaatg agaacgaaat ggccaaagaa
    13021 agggcattaa cgctggaagc actacgggtt atggatgcga tcgatcgccg gggcagtttt
    13081 gcggcggcgg cggatgagct gggacgcgtg ccttccgcac ttagctacac catgcaaaaa
    13141 ctggaagaag agctggatgt ggtgctgttt gaccgctcgg gccatcgcac caaattcacc
    13201 aatgtcggga ggatgttgct ggagcgaggg cgcgttttgc tggaagcagc agataaactg
    13261 actaccgatg cggaagccct cgcgcgcggt tgggaaacgc atctcaccat tgtgaccgag
    13321 gcgctggtac cgacacctgc ctttttcccg ttaatcgaca aactggcggc aaaagccaat
    13381 acccaactgg caatcatcac cgaagtgctg gcgggggcgt gggaacgact ggagcagggg
    13441 cgggcggata tcgttatcgc gccggatatg cattttcgtt cctcgtcgga gatcaactcg
    13501 cgcaagctct ataccttaat gaacgtctac gttgccgcgc ctgatcaccc gattcatcag
    13561 gagccggaac cgttatctga agtgacgcgc gtgaaatatc gtggaattgc ggtagcggat
    13621 accgctcgtg agcgcccggt gttgaccgta cagctgctgg acaaacagcc gcgcttaacg
    13681 gtgagcacga ttgaagataa acgtcaggca ttactggcgg ggcttggcgt ggcgacgatg
    13741 ccgtatccga tggtcgaaaa agatattgcg gaagggcggt tgcgtgtcgt cagcccggaa
    13801 tcgaccagcg agatcgatat tattatggcc tggcgtcgtg acagtatggg ggaagcgaaa
    13861 tcctggtgtc tgcgggaaat tcccaaactt tttagcggaa aataatattt attattgtta
    13921 ataaaaaggg gcgaaatatc gccccaattc taagattaat ttgaaccaaa cttaggatcg
    13981 tttccgtaac gattgctgcc agaattgcct tctaaacagg caaatacaaa cagaactaac
    14041 cagccaatga tcggtaccag atagagaagc gcccacacac ctgaacggtc agtatcgtgc
    14101 agacgacgaa cacacagagc aatgaccgga atgattgttg ctgccaggta aatgagagaa
    14161 aggaatggaa actctaaacc taaaatcaat tgaatgacat tgataatagc gccgacgatg
    14221 gcgttaatca gagtaaacat ccagtactct ttacgacgtg cgcgaccaga gaaaccaaca
    14281 taatttttta atacggctaa ataccactgc ataaaaacat ccctttaatt aaaagtgacg
    14341 aaagtctatt ccgggtattg tgtaaatgct attcgtatct ttctttttc
//
LOCUS       DAZPEB010000102        14198 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZPEB010000102 DAZPEB010000000
VERSION     DAZPEB010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14198
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.103"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            672..2303
                     /gene="oppA"
                     /locus_tag="ACHURJ_002589"
     CDS             672..2303
                     /gene="oppA"
                     /locus_tag="ACHURJ_002589"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFR1281483.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2389..3309
                     /gene="oppB"
                     /locus_tag="ACHURJ_002590"
     CDS             2389..3309
                     /gene="oppB"
                     /locus_tag="ACHURJ_002590"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFR1281484.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3324..4232
                     /gene="oppC"
                     /locus_tag="ACHURJ_002591"
     CDS             3324..4232
                     /gene="oppC"
                     /locus_tag="ACHURJ_002591"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFR1281485.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFIHNRAAVASLM
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            4244..5257
                     /gene="oppD"
                     /locus_tag="ACHURJ_002592"
     CDS             4244..5257
                     /gene="oppD"
                     /locus_tag="ACHURJ_002592"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFR1281486.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTLGRLCACFKPVE
                     ELL"
     gene            5254..6258
                     /gene="oppF"
                     /locus_tag="ACHURJ_002593"
     CDS             5254..6258
                     /gene="oppF"
                     /locus_tag="ACHURJ_002593"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFR1281487.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGIKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6311..6640)
                     /locus_tag="ACHURJ_002594"
     CDS             complement(6311..6640)
                     /locus_tag="ACHURJ_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFR1281488.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPVEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6675..8135)
                     /gene="cls"
                     /locus_tag="ACHURJ_002595"
     CDS             complement(6675..8135)
                     /gene="cls"
                     /locus_tag="ACHURJ_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFR1281489.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEIVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            8278..8451
                     /locus_tag="ACHURJ_002596"
     CDS             8278..8451
                     /locus_tag="ACHURJ_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFR1281490.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8506..9759)
                     /gene="kch"
                     /locus_tag="ACHURJ_002597"
     CDS             complement(8506..9759)
                     /gene="kch"
                     /locus_tag="ACHURJ_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFR1281491.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCLAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(10059..10355)
                     /locus_tag="ACHURJ_002598"
     CDS             complement(10059..10355)
                     /locus_tag="ACHURJ_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFR1281492.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            10570..11298
                     /gene="tonB"
                     /locus_tag="ACHURJ_002599"
     CDS             10570..11298
                     /gene="tonB"
                     /locus_tag="ACHURJ_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFR1281493.1"
                     /translation="MTSMTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTALARLTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
     gene            complement(11338..11736)
                     /gene="yciA"
                     /locus_tag="ACHURJ_002600"
     CDS             complement(11338..11736)
                     /gene="yciA"
                     /locus_tag="ACHURJ_002600"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFR1281494.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(11841..12380)
                     /locus_tag="ACHURJ_002601"
     CDS             complement(11841..12380)
                     /locus_tag="ACHURJ_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFR1281495.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(12410..13153)
                     /locus_tag="ACHURJ_002602"
     CDS             complement(12410..13153)
                     /locus_tag="ACHURJ_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFR1281496.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAVLLIYLFRLYMLIRQ"
     gene            complement(13181..13270)
                     /locus_tag="ACHURJ_002603"
                     /pseudo
     CDS             complement(13181..13270)
                     /locus_tag="ACHURJ_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            13510..14148
                     /gene="ompW"
                     /locus_tag="ACHURJ_002604"
     CDS             13510..14148
                     /gene="ompW"
                     /locus_tag="ACHURJ_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFR1281497.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
BASE COUNT         3826 a         3318 c         3474 g         3580 t
ORIGIN      
        1 cgcagttatc aaaagcaaaa ggaataggta aaaatattct tctcaaatta cagttagtta
       61 taaggatttt cttaactgct tctcctcacc atcatgttat tttcgccaca tcataatcct
      121 gggcttgctg aagaataatt gaaatgatat tagtaattcc actgcctttg gtagaggaaa
      181 gtgctaaata ataatcaatt gttaaattat tgtgcatttc aatactgaaa ctgtaatcag
      241 aaaagataga catgcttagc caatctctat ttgattgaat tgaaagatgt ttgttaaggc
      301 atggatgcaa gctatagatt ctgatacggt caataaaaga gaattgctta acaattttgc
      361 aaaatgtatt ggcgagtaag aaccgcattt agtactttcc gggcaaccgc cagacgattc
      421 tttattggta atgagaataa ttaacaatta aagagcgtcg cgaaagaata atgtgtctcg
      481 acaggggaga cacagtacga atcgacataa ggtgatcgtc tgaatcacca gaataaataa
      541 agtcggtgat agcaatccgt aacgataaag taacctgaca gcagaaagtc tccgagcctg
      601 tgcagggtcc caatccggga ttacacatgc tggttaatac cagtaattat aatgagggag
      661 tccaaaaaac aatgaccaac atcaccaaga gaagtttagt agcagctggc gttctggctg
      721 cgctaatggc agggaatgtc gcgctggcag ctgatgtacc cgcaggcgtc acactggcgg
      781 aaaaacaaac gctggtacgt aacaatggtt cagaagttca gtcattagat ccgcacaaaa
      841 ttgaaggtgt tccggagtct aatatcagcc gagacctgtt tgaaggctta ctggtcagcg
      901 atcttgacgg tcatccggca cctggcgtcg ctgaatcctg ggataataaa gacgcgaaag
      961 tctggacctt ccatttgcgt aaagatgcga aatggtctga tggtacgcca gtcacagcac
     1021 aagactttgt gtatagctgg caacgttctg ttgatccgaa cactgcttct ccgtatgcca
     1081 gttatctgca atatgggcat atcgccggta ttgatgaaat tcttgaaggg aaaaaaccga
     1141 ttaccgatct cggtgtgaaa gctattgatg atcacacatt agaagtcacc ttaagtgaac
     1201 ccgttccgta cttctataaa ttacttgttc acccatcaac ttcaccggtg ccaaaagccg
     1261 ctatcgagaa attcggcgaa aaatggaccc agcctggtaa tatcgtcacc aacggtgcct
     1321 ataccttaaa agattgggtc gtaaacgaac gaatcgttct tgaacgcagc ccgacctact
     1381 ggaacaacgc gaaaaccgtt attaaccagg taacctattt gcctattgct tctgaagtta
     1441 ccgatgtcaa ccgctaccgt agtggtgaaa tcgacatgac ttataacaac atgccgatcg
     1501 aattgttcca gaagctgaaa aaagagatcc cggacgaagt tcacgttgat ccatacctgt
     1561 gcacttacta ttacgaaatt aacaaccaga aaccgccatt caacgatgtg cgtgtgcgta
     1621 ccgcactgaa actaggtatg gaccgcgata tcattgttaa taaagtgaaa gcgcagggcg
     1681 acatgcccgc ctatggttac actccaccgt atactgatgg cgcaaaattg actcagccgg
     1741 aatggtttgg ctggagccag gaaaaacgta acgaagaagc gaaaaaactg ctggctgaag
     1801 ctggttatac cgcagacaaa ccgttgacaa tcaacctgtt gtataacacc tccgatctgc
     1861 ataaaaagct ggcgattgct gcctcttcat tgtggaagaa aaacattggt gtaaacgtca
     1921 aactggttaa ccaggagtgg aaaacgttcc tcgacacccg tcaccagggt acttttgatg
     1981 tggcccgtgc aggctggtgt gctgactaca acgaaccaac ttccttcctg aacaccatgc
     2041 tttcgaacag ctcgatgaat accgcgcatt ataagagccc ggcctttgac agcattatgg
     2101 cggaaacgct gaaagtgact gacgaggcgc agcgcacagc tctgtacact aaagcagaac
     2161 aacagctgga taaggattcg gccattgttc ctgtttatta ctacgtgaat gcgcgtctgg
     2221 tgaaaccgtg ggttggtggc tataccggca aagatccgct ggataatacc tatacccgga
     2281 atatgtacat tgtgaagcac taatggcaat acgtggggca ggagtgtcct gctccacggt
     2341 gtctgatttt tatcgcatta cagaaggcac aggccagaag gtagggcaat gttaaaattt
     2401 attctacgtc gctgtctgga agcgattccg acgctattta ttcttattac tatttcgttc
     2461 tttatgatgc gcctcgcgcc gggaagccct tttaccggcg aacgtacttt accgccagaa
     2521 gtgatggcca atatcgaagc gaaatatcat cttaatgatc cgatcatgac acagtatttc
     2581 agctacctga aacaactggc gcacggtgat ttcggtccat cgtttaaata taaagattat
     2641 tcggtcaatg acttggttgc atccagtttt cccgtttctg ccaaactggg agccgcagca
     2701 tttttccttg cggtaatact gggtgttagt gctggcgtta ttgccgcatt aaaacaaaac
     2761 accaaatggg actataccgt gatggggctg gcaatgaccg gggttgttat ccccagtttt
     2821 gtggttgcac cattattagt catgatattt gcgatcattt tgcattggct gccgggcggt
     2881 ggctggaatg gtggggcgct taaattcatg atattgccga tggtggcgtt gtcactcgct
     2941 tatatcgcca gtattgcgcg tattacccgt ggctctatga ttgaagtatt acactccaat
     3001 tttattcgta ctgcccgggc gaaagggtta cctatgcggc ggatcatttt acgtcacgca
     3061 ttaaaacctg ctctgttacc cgtgctctct tatatgggcc ccgcatttgt cggcattatt
     3121 accggttcta tggttatcga aaccatttat ggtttgccgg ggattggaca actgttcgtt
     3181 aatggcgcat tgaaccgtga ctattcctta gtgttaagcc tgaccatcct ggttggtgct
     3241 ttaaccattt tgtttaatgc cattgtcgat gtgctatatg cggttatcga cccgaaaatc
     3301 cgttactgat actggagctc gcgatgatgt taagtaagaa aaacagcgag acgctggaaa
     3361 atttcagtga aaagctggag gtcgaagggc gcagcttgtg gcaggacgca cgtcgacgtt
     3421 ttattcataa ccgtgcggcg gttgccagtc tgatggtgct ggtgctgatc gcgttatttg
     3481 taatcctggc accgatgctt tcccagtttg cctatgacga tactgactgg gcgatgatgt
     3541 ccagcgcccc ggatatggag tccggtcact actttggtac tgactcatcc ggtcgcgacc
     3601 tgcttgtgcg cgttgcgatt ggtgggcgta tctcactcat ggtcggtgtt gctgcggcac
     3661 tggtggctgt ggtcgtcggg acactttatg gttcgctttc cggttatctg ggcggtaaag
     3721 tggattcggt aatgatgcgt ctgctggaaa tcctcaactc cttcccattc atgttcttcg
     3781 tcattttgct ggtgaccttt ttcggtcaaa acatcctgct gattttcgtg gcgataggca
     3841 tggtttcctg gctggatatg gctcgtattg tgcgtgggca aaccctgagt ctgaagcgca
     3901 aagagtttat tgaggcggca caagttggcg gtgtatcgac atcgggcatt gttattcgcc
     3961 acattgtgcc gaatgtactc ggtgtggtgg tggtctacgc atcacttctg gtgcccagca
     4021 tgatcctctt tgaatctttc cttagcttcc tggggttggg tacgcaagag ccgttaagca
     4081 gctggggtgc attgctgagt gatggcgcga actcgatgga agtctctcca tggttattgt
     4141 tgttcccagc gggattcctc gtggtgacgc tgttttgttt caactttatc ggcgatggct
     4201 tgcgtgatgc cctcgacccg aaagatcgtt aaggagtgca gccatgagcg taattgaaac
     4261 tgcaactgtg ccgctcgcac aacaacaggc tgacgcactg ctgaacgtga aagatttgcg
     4321 tgtcaccttt agtaccccgg acggcgacgt cacggcggtc aatgatttga atttttccct
     4381 acgtgccgga gaaacgctgg gtattgtagg tgagtctggt tcgggtaaat cgcaaactgc
     4441 atttgcgttg atgggcctgt tggctgccaa cggacgtatt ggcggatcgg caaccttcaa
     4501 tgggcgtgaa atcctcaatt tgccagagca tgaactcaat aaactgcgcg ctgaacaaat
     4561 ctcaatgatt tttcaggacc caatgacttc gttgaatccc tatatgcgcg tcggtgagca
     4621 gttgatggaa gtgctgatgc tgcataagaa catgagcaaa gctgaggcgt ttgaagagtc
     4681 ggtgcggatg ctcgatgcgg taaaaatgcc ggaagcacgt aaacgcatga aaatgtaccc
     4741 gcacgagttt tctggcggca tgcgtcagcg agttatgatt gcgatggcat tgctatgtcg
     4801 acctaagctg ctgattgcgg atgaacccac tacagcgctg gacgtcaccg tacaggcgca
     4861 gatcatgacg ctattaaatg aactgaaacg ggaatttaat accgccatca ttatgattac
     4921 ccacgatctt ggcgtggtgg cggggatctg tgacaaagtg ctggtaatgt acgccgggcg
     4981 cacgatggaa tatggcaacg cgcgcgatgt cttttatcaa cccgttcatc cttattctat
     5041 cggtttgctc aacgcggtgc cgcgtctcga tgcggaaggt gaaacaatgt tgaccatccc
     5101 gggtaatccg ccaaacctgc tgcgattacc gaaaggttgc ccgttccagc cacgttgtcc
     5161 gcatgcgatg gaaatttgta gtagcgcacc gccgctggaa gagtttacgc ttggccgtct
     5221 gtgtgcttgc tttaaaccgg tggaggaact gttatgaatg ctgtaaccga aggaagaaaa
     5281 gtcctccttg aaatcgccga tctgaaagtg cactttgaaa tcaaagatgg caaacagtgg
     5341 ttctggcaac cgccgaaaac gctcaaagcc gtcgatggtg tcacgcttcg cctgtatgaa
     5401 ggggaaacat taggtgtggt aggggaatcg ggatgcggta agtccacctt tgctcgcgcc
     5461 atcattggcc tggttaaagc gaccgacggt catgttgcct ggttaggtaa agagttgctg
     5521 ggcattaagc ccgatgaatg gcgtgccgtt cgcagtgata ttcagatgat tttccaggat
     5581 ccgttggcat cgctaaaccc gcgcatgacc atcggcgaga tcatcgctga accactgcgt
     5641 acttatcatc cgaaaatgtc acgtcaggaa gttcgcgagc gcgtgaaggc gatgatgctg
     5701 aaagtcgggt tattgcctaa cctgattaac cgctatccgc atgagttctc cggtgggcag
     5761 tgccagcgta tcgggattgc tcgtgctctt attcttgaac cgaagctgat tatctgtgat
     5821 gagccggtgt cggcgctgga cgtgtcaatt caggcgcagg tggtcaacct gctccagcag
     5881 ctgcaacgtg agatgggatt gtcattaatt tttattgctc atgacctggc cgtggtaaaa
     5941 cacatttccg atcgtgtgtt ggtgatgtat ctcggccatg cggtagaact ggggacctat
     6001 gatgaggtct accacaatcc actacatcct tacaccaggg cattgatgtc ggcagtcccc
     6061 atacctgatc cggatctgga gaagaacaaa accatccagt tactggaagg ggaattaccg
     6121 tcgccgatca acccgccttc cggttgtgtt ttccgtaccc gttgcccgat tgccggtccg
     6181 gagtgcgcca aaacacgtcc tgtgctggaa gggagtttca gacacgccgt ttcttgcctg
     6241 aaagtcgatc cgctttaaac acaataaggg ctgacagttg tcagcccttt ttcacgccga
     6301 aagcgattat ttattcccgc cagatgatat ggcaaagttt gtgatctttt tcgcggcata
     6361 acagaatgcg ggcgaaaaca tcgttgattt caccatcctc actgtccgcc agaccaatca
     6421 ccacttcggc aaaaaagtcc gggttcaaat cgaaatcaac atgttcctgc cagtcttcca
     6481 ccggatcgaa taactccgcg ccgccgcgct cttcaaactg aagattgaac agcagaacat
     6541 cagctgggtc gagattgtcg gcagccagtt cgagaaaaat atcgtaggcc tgctcaagcg
     6601 tttcatcttc agtcaggcga ttgtttagat ccatatccat agtcactacc tgtttaacct
     6661 ctgttggaga cgttttacag caacgggctg aagaagtaaa acagtcgctc ggcgacacgt
     6721 tgccataatg gacgttttag ccataaacgg gcatcgagca gacgtgaacg cgaaatataa
     6781 tcgtcctgaa cggcggcaag gtctgcacca aaacctttat cgtcgattgc cagggtaatc
     6841 tcgaaattca gccacagact acgcatatca aggttaacgg tgccaaccag acttagttcg
     6901 ccatcgacca gcacgctctt ggtatgcagt aacccgcctt caaactgata aattttaacc
     6961 ccagcagcaa gaagttccgt aaagaatgcg cgactggccc agccgaccag catcgagtca
     7021 ttttttcgcg gaaggataat actgacatcc accccgcgct gtgccgccgt gcaaatcgca
     7081 tgaagtaaat catcgcttgg cacaaagtag ggcgtggtca tgatcaaata ttcacgcgcc
     7141 gaataagccg cagtcaataa tgcctggtga atgagatctt ccggaaagcc ggggccagaa
     7201 gcaattgtgt gaatggtgtg accgctggcc tgttcaaacg gcataatatt gacatctggt
     7261 ggtggcggca gaatacgttt tccggtttca atctcccagt cgcaggaata aataatcccc
     7321 atcgcggtgg cgatggggcc ttccatacgc gccatcagat caatccattg ccctacgccc
     7381 gcatcttgtt tgaagtagcg aggatcgacc atattcatgc tgccggtgta cgcgatgtaa
     7441 ttatcgatca tgatcatctt gcgatgttgg cgcaggtcca tacggcgtaa aaacacacgc
     7501 atcagattga cctttaaggc ttcgaccact tcaataccgg cattacgcat tagctcgggc
     7561 cacgggctgc ggaaaaaagc cacactcccg gcggagtcga gcatcaatcg gcaatgaatg
     7621 ccgcgtcgcg cagcggccat taatgattca gccacctggt ccgccatgcc gccgggctgc
     7681 cagatataaa acacaatctc aatattatgg cgcgcgagct ggatgtcgcg gattaacgcc
     7741 tgcatcacat catctgactc ggtcatcagt tgtagctgat tccctttgac cccagcgatc
     7801 ccctgacgac gctcgcaaag cttgaataat ggcgcagcga cactgctatt ttcttcggcg
     7861 aagatatgct tacaggcttt aaggtcgtta agccattttg cggtggaagg ccacatcgct
     7921 ctggcgcgct cagcgcggcg tttgcctaaa tggagctcgc caacggcaag ataggcaata
     7981 attccgacta acggcagaat gtaaataatc aacagccagg ccatcgcgga gggaactgcg
     8041 cgtcgtttca ttagaatgcg taaagttacg cctgcaatga gcaaccagta tcccagaatg
     8101 gccaaccaac tcaccaacgt ataaacggtt gtcatagatt agaaatcctt ttgaaagcgc
     8161 attgttatga gtttacgcat atggattcat ctggcaaata aaaacgcggg aaaagcactg
     8221 gtcagcgtcg ggggcggggg tataatgacc attctgttat tgcatagagt agttaacatg
     8281 aagcggagta gaacggaagt ggggcgctgg cgcatgcagc gtcaggctag ccgacgtaaa
     8341 tcgcgttggc ttgaggggca atcgcgccga aatatgcgta tccacagcat caggaagtgc
     8401 attctaaaca aacagcgtaa ctcgttattg tttgcgatct acaatatcta aatgtactgg
     8461 gcaccattgc ggtgcccaat aagtttactc ctccatcttt aaactctatt tttgcgccga
     8521 ttctttactg tcagctttcg tttcttgttc atcgttatcg ctgaaaatgc catgaccgga
     8581 gttcaacaac attgaaacga gcatatcatt attaatctct tcaccattta aaactcgcgc
     8641 cagaatttcg ctgccaaaca gttgcggtga gagaatgata tccggatgta ccatcttaat
     8701 cttattcagg tttttgctat cactgacggc gagaactgtt ttgacatcac tgctcatatc
     8761 ttttgccgag agtacaacaa acgcgttatc tgcatcgtta tcactcagcg ccagaatggc
     8821 caggcaacga tcgattcccg ctttctttaa tactgaactg tcattactgt caccggggat
     8881 aacatcagcg ttatcgccta agcgttgctc aagttgcttg atatcatctt caggcaagtt
     8941 gctgataacc gttacgtttt gtccgcgttg attcagttgc agaatcgtat tgatggcgag
     9001 aatcgaatgt ccgcaaacaa taaaatgatc tttacgatgc attgtatgat tgtttccttt
     9061 tacaagtttg ttgaatcccc cgcggataag cggaccaaaa attgaggtca tagatgtggc
     9121 aaaaacggta ataccggaaa taatgaccga aatagtgaac aatcgtgctg attcagaaac
     9181 agggacaata tcgccatagc cgacggttga catggtttct atcgaaaaat agaacgcggt
     9241 catcaaactt tctattcgcg gattaaaacc ttcgcttaaa taaagcgcac cgtaggttga
     9301 gtaaaacagt aatgtcgtga aactaataaa tgcaaaaatt gtcccggctg cggcgctact
     9361 gtgggagaag tctttgcgca gtatcagcaa aaacaccagc gtaaaaatgc aaaatccaat
     9421 actaaatttc aaccagggat aaaaatgcag ggtataaatt agcgctatca acaacaaaat
     9481 gatactgatt gcccatgcga gctttgcccg gaacagtaag ccaatggagt tcaggacaag
     9541 aaagacgccg agcataaata gtggggcgtt tgccagcaag gagagatcca actctgaaaa
     9601 tgctttgata tggaaaatat ctagcaaatt gaccgacata ccatagattg ttttaaaaat
     9661 aagcaatcca tttaaaaaga cggccagcgc gagaatgtcg tgccgtaggg tcacccataa
     9721 atttgttgct gtttgtttga atgtagccca gtgactcact gatacctctg taaccacaat
     9781 tttgtttaat tatcttagcg tgcacatttt ctgaacatac atgcagcgcg tgaatgtgtt
     9841 aaacaatagt agatgacttt atccgcagta atcaaaggtt ttgagcgagc aactgtacct
     9901 caggaaaggt catttttcct gaatatgctc acatcatata aagaaataca gataaagtta
     9961 ttatctgctt gtggtggtga atgcactgac cggctataag gaaaggccaa acaagaacac
    10021 ggttgcaaaa accgtgccct taaatattga atctctattc agaacacttt cttaaatggt
    10081 ttcactgaaa cgtgttcata gactcctgcc gctacgtacg ggtcagcatc ggcccaggcc
    10141 tgagctgctt ccagcgattc aaattcagca ataacggttg agccagtaaa tcccgcagcc
    10201 cctggatcgt tactgtctac cgctggcatt ggaccagctg tcaacaaacg accttcatca
    10261 tgcagtaact gtaaacgtgc taaatgtgcc ggacgaacgg aaaggcgttt ttcgaggcta
    10321 tcagctttgt cttgagcgta aataacatac aacacgggca caactccttg ttcgggaaag
    10381 ttgtaaagta cgttatttga aagggcgaag atctgcaacg gaaagatgat gtctttgtta
    10441 agaccatgca taaagtaagg ttaattacgg caaaaatgac attttcactg atcctgatcg
    10501 tcttgcctta ttgaatatga ttgctatttg catttaaaat cgaggcttgg tttttctact
    10561 gaaatgatta tgacttcaat gacccttgat ttacctcgcc gcttcccctg gccgacgtta
    10621 ctttcggtct gcattcatgg tgctgttgtg gcgggtctgc tctatacctc ggtacatcag
    10681 gttattgaac tacctgcgcc tgcgcagcca atttctgtca cgatggttgc gcctgctgat
    10741 ctcgaaccgc cacaagccgt tcagccgcca ccggagccgg tggtagagcc agaaccggaa
    10801 cctgagccga tccccgaacc gccaaaagaa gcaccggtgg tcattgaaaa gccgaagccg
    10861 aaacctaagc caaaaccgaa gccggtgaaa aaggtacagg agcagccaaa acgcgatgtc
    10921 aaacccgtag agtcgcgtcc ggcatcaccg tttgaaaata cggcactggc acgcctgaca
    10981 tcaagtacag caacggctgc aaccagcaag ccggttacca gtgtggcttc aggaccacgc
    11041 gcattaagcc gtaatcagcc gcagtatccg gcacgagcac aggcattgcg cattgaaggg
    11101 caggttaaag ttaaatttga cgtcacgccg gatggtcgcg tggataacgt acaaatcctc
    11161 tcagccaagc ctgcgaacat gtttgagcgt gaggtgaaaa atgcgatgcg cagatggcgt
    11221 tatgagccgg gtaagccagg cagtgggatt gtggtgaata tcctgtttaa aattaacggc
    11281 accaccgaaa ttcagtaagc agaaagtcaa aagcctccga ccggaggctt ttgactatta
    11341 ctcaacaggt aaggcgcgag gttttccttc aggatcaacc gcgacatact taaataatgc
    11401 ttctgtcgct ttatagcgtt gcccgattgg ttcagacgct acttttttca cccacacttc
    11461 aatattaatg ctgaccgatg tcgtcccttt ctggacacag cgtgcatagc agcacactac
    11521 atcgccgacc gcaaccggcc gtaagaaagt cattccttca acccgcacgg tcactacgcg
    11581 accgtgggca atttctttcg ccagaatagc gccgccaata tccatttgtg acattaacca
    11641 accaccaaag atgtcaccat tggcattggt atcggcgggc atggctaaag tacgtaaaac
    11701 aagatcgccc tgagggacgt tatgtgttgt agacatggta aaaccgactt aaaaggaaaa
    11761 tcacaggcgc gatgctacta tgattttcct gaggagaaca gaggagatac agcgccagcc
    11821 ccgaaggact ggcagtctgg ttaggattta tcttcctgcg gcatgtggcg gtagatatag
    11881 ataccgctta acaatgtgaa gattaaggta agggcggtca gtccaaagac tttaaagttg
    11941 acccaaatat tttgcggcag ccagaatgcg atgtagatgt ttgccagacc gcaaaggata
    12001 aagaaaacag cccaggccag attcagcttc gaccataccg gttgcggcag cgtgagttct
    12061 ttacccagca tccgctgaat tagcggcttt ttcatcaccc attggctgac taacagggct
    12121 cccgcaaaca gagcataaat gactgtaacc ttccatttaa taaactcatc attgtggaag
    12181 aacagcgtca agccaccgaa gacgaccacc agaacaaaag tgatcagggc catcttctca
    12241 accttacgaa agcgaaccca gctatagata agcacaatcg ccgtggcgac gatcagcgcc
    12301 gcagtagccg catagatgtc ataaatcttg taaaacgcga aaaagacaac cagcggtaaa
    12361 aaatcaagaa actgcttcat tttacgattc cgtaatcaaa tgcttaaggt tattggcgaa
    12421 tcaacatata cagacggaac agatagatga gcaatacggc tgaaatcagg ttgctcaagg
    12481 tgttcgccag tacggcacca atttccgggg ttaatgcggc aaaagaagag gcaaaaagca
    12541 gcaacagtgt ttttgccagc aaccagctca gtactgcggg tgccaccaga cgcatattcg
    12601 cccaagtcag ccgcatactg ctacgcatcg aggcaaaaat gcccattttg tcctgtacca
    12661 gcatcaccgg tgccagggcc agtaaaatgg ccatgataat tcccggaacg accactagca
    12721 tgatgccaat ctgtactaaa agggtagtca gaaaaatcag aataaataac tttggcaata
    12781 tcggtgcact ggcaccaata gctcgcagcg cactgactct ctgacccgca gacaccagct
    12841 ggataattaa tattacgcct ccggcgagaa tggcgttacc gattaatcct gaaaaagtgg
    12901 acgccgctga agcctgcagc aaaatttgtt gctgttccgg tgacatattc tgaaccaggt
    12961 cgaacaaccc actactgccg ctaacgggca cgccgtcatt gagctgcgca agctgtgcat
    13021 cactgggtga gaaaacatgc cctaacacca ctgtgataaa cgcacatagc aacgatacca
    13081 acagaatggt cataaattga ttacggaaga aatttccggt gtcacggtat acggactgcg
    13141 ccgtgataga catgcactct ccttgagttt tatgcaggtg ttaattagcg ggcaattgta
    13201 ccctggttaa gcctcgggtg gcagcatcag gtcttgtatg gaaaagcata tctttgtaaa
    13261 gcggaggtaa ttgctggcct tcaggcgtgg gcaaaccgcg gcaagtggcg atcgagttaa
    13321 tatttgcgta gcgaaaatat ttaaaaattg atttaaatca cgttaaccag gattctcaat
    13381 gcaacttcta aattaatcca gatcaataaa gggtgaatta tcatatgtaa tgtgatctat
    13441 gtaggatcat ttgttactcc aatgtaggta tattcgtcac gtttttataa ccataacgac
    13501 ggagcggata tgaaaaagtt aacagtggcg gctttggcag taacaactct tctctctggc
    13561 agtgcctttg cgcatgaagc aggcgaattt tttatgcgtg caggttctgc aaccgtacgt
    13621 ccaacagaag gtgctggtgg tacgttagga agtctgggtg gattcagcgt gaccaataac
    13681 actcaactgg gcttgacgtt tacttatatg gcgaccgaca acattggtgt ggaattactg
    13741 gcagcgacgc cgttccgcca taaaatcggc acccgggcga ccggcgatat tgcaaccgtt
    13801 caccatctgc cgccaacact gatggcgcag tggtattttg gtgatgccag cagcaaattc
    13861 cgtccttacg ttggggcagg tattaactac accaccttct ttgataatgg atttaacgat
    13921 catggcaaag aggcagggct ttccgatctc agtctgaaag attcctgggg agctgccggg
    13981 caggtggggg ttgattatct gattaaccgt gactggttgg ttaacatgtc agtctggtac
    14041 atggatatcg ataccaccgc caattataag ctgggcggtg cacagcaaca tgatagcgta
    14101 cgcctcgatc cgtgggtgtt tatgttctca gcaggatatc gtttttaatt ccgcacaaaa
    14161 acgaccccgt aatatacggg gtcaataagg acatggca
//
LOCUS       DAZPEB010000103        13890 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZPEB010000103 DAZPEB010000000
VERSION     DAZPEB010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13890
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.104"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            220..1167
                     /locus_tag="ACHURJ_002605"
     CDS             220..1167
                     /locus_tag="ACHURJ_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFR1281498.1"
                     /translation="MKNVLVTVPSFSARCVSASKLLRENNFNLIIKNNVEHLLKSEST
                     ALRESICAVIAGKDCYQADTLSLLPGVKIISRFGTGIDNIDLRAAQQSGIVVNNAVGI
                     NSNAVAEFIIGLIFASMRNIPGSYHAMQNGYWGESHGYELQGKRIGMVGYGNIGKTLA
                     KRLSGFDVELLAYDKQPDYQVADKVGVQFVSIEDIFMQSHVIIVLLPFSPELENFISH
                     KYLSMMRNGALIINAARGKLLDEGALLQVIEERNVFAALDVFSSEPLAQFSPLLHAKN
                     IITTPHIAAATVESYQQTGTHVAQSIIDYFAGREIKNVL"
     gene            complement(1246..1322)
                     /locus_tag="ACHURJ_002606"
     tRNA            complement(1246..1322)
                     /locus_tag="ACHURJ_002606"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1286..1288),aa:Met,seq:cat)
     gene            1531..2628
                     /gene="mltA"
                     /locus_tag="ACHURJ_002607"
     CDS             1531..2628
                     /gene="mltA"
                     /locus_tag="ACHURJ_002607"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFR1281499.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKSTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            2705..3511
                     /gene="tcdA"
                     /locus_tag="ACHURJ_002608"
     CDS             2705..3511
                     /gene="tcdA"
                     /locus_tag="ACHURJ_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFR1281500.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSIGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(3562..4005)
                     /gene="csdE"
                     /locus_tag="ACHURJ_002609"
     CDS             complement(3562..4005)
                     /gene="csdE"
                     /locus_tag="ACHURJ_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFR1281501.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVLGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRGQLSASRSQGLNALSEAIIAAAKQV"
     gene            complement(4005..5210)
                     /gene="csdA"
                     /locus_tag="ACHURJ_002610"
     CDS             complement(4005..5210)
                     /gene="csdA"
                     /locus_tag="ACHURJ_002610"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFR1281502.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALSQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAENWSHSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            5402..5629
                     /locus_tag="ACHURJ_002611"
     CDS             5402..5629
                     /locus_tag="ACHURJ_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFR1281503.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            5980..6897
                     /gene="gcvA"
                     /locus_tag="ACHURJ_002612"
     CDS             5980..6897
                     /gene="gcvA"
                     /locus_tag="ACHURJ_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFR1281504.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            6916..7311
                     /locus_tag="ACHURJ_002613"
     CDS             6916..7311
                     /locus_tag="ACHURJ_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFR1281505.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            7304..8404
                     /gene="rlmM"
                     /locus_tag="ACHURJ_002614"
     CDS             7304..8404
                     /gene="rlmM"
                     /locus_tag="ACHURJ_002614"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFR1281506.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENSGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(8448..9179)
                     /gene="fucR"
                     /locus_tag="ACHURJ_002615"
     CDS             complement(8448..9179)
                     /gene="fucR"
                     /locus_tag="ACHURJ_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFR1281507.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICYELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(9237..9659)
                     /gene="fucU"
                     /locus_tag="ACHURJ_002616"
     CDS             complement(9237..9659)
                     /gene="fucU"
                     /locus_tag="ACHURJ_002616"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFR1281508.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(9661..11079)
                     /gene="fucK"
                     /locus_tag="ACHURJ_002617"
     CDS             complement(9661..11079)
                     /gene="fucK"
                     /locus_tag="ACHURJ_002617"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFR1281509.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERTHAWFFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     SADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(11076..12821)
                     /gene="fucI"
                     /locus_tag="ACHURJ_002618"
     CDS             complement(11076..12821)
                     /gene="fucI"
                     /locus_tag="ACHURJ_002618"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFR1281510.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEVLQERL"
     gene            complement(12854..>13890)
                     /gene="fucP"
                     /locus_tag="ACHURJ_002619"
     CDS             complement(12854..>13890)
                     /gene="fucP"
                     /locus_tag="ACHURJ_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFR1281511.1"
                     /translation="ITGLFLYALGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAA
                     NPFVTVLGPESSGHFRLNLAQTFNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQ
                     LSAYKHSLVLSVQTPYMIIVAIVLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRL
                     ARIRHWRWAVLAQFCYVGAQTACWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGR
                     FTGTWLISRFAPHKVLAAYALIAMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFS
                     LGIKNLGQDTKYGSSFIVMTIIGGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIF
                     ARFRSQTATN"
BASE COUNT         3363 a         3407 c         3653 g         3467 t
ORIGIN      
        1 aggtttagca ccgtcatgct cgtctactgt tgttctggtt gttggtgatg cggtggcgct
       61 tgcactctct gaattgaaaa aatttactcg tgctgatttc ggcttatatc atccaggagg
      121 tgcactcggc attaaagcaa attcataatt tcaaagtata aatacagtga ccggtcacgc
      181 catcttttgg ggtggcgttc attgaagtga gtgtcagcta tgaaaaatgt tctggtcacc
      241 gtccccagct tttcagcacg ctgtgtttcc gccagcaagt tgctaagaga gaataatttt
      301 aatcttatta ttaagaataa tgtggagcat ctgcttaaat cagaatccac tgcattacga
      361 gaatctattt gcgcggtcat cgccggaaaa gattgttatc aggcggacac gctatcattg
      421 ctgcctggtg tgaaaataat atcgagattc gggacgggga tagataatat tgatcttcgt
      481 gcagcacaac aatccggtat tgtagtcaat aatgcggttg ggattaattc aaatgctgtc
      541 gcggaattta tcattggact tatcttcgcg agcatgagaa atattcccgg cagctatcat
      601 gcgatgcaaa atggctactg gggtgagtcg catggctatg aattacaagg caaacggatt
      661 ggcatggttg gctacggcaa tataggtaaa accctggcaa aaaggttatc cggttttgat
      721 gttgagcttt tagcttatga caaacaaccc gattatcagg ttgctgacaa agttggagtt
      781 cagtttgtat caattgaaga tatctttatg cagtcgcatg tcatcattgt tcttttgcct
      841 ttttctcctg agctggagaa cttcattagt cataaatatt tgtcgatgat gcgcaatggt
      901 gctctaatca tcaatgccgc aaggggaaag ttgctggatg agggcgcttt actacaggtg
      961 attgaagaac gaaatgtgtt tgcggcgctg gatgtgttta gctctgaacc gttggcgcaa
     1021 tttagcccac tgctacatgc caaaaatatc attacaacgc cgcatattgc ggccgccacc
     1081 gttgagtcat accagcaaac cggtacacac gttgcccagt cgataattga ttacttcgca
     1141 ggaagggaga taaaaaacgt gttgtgagtt cagcgaacga agtttttaaa tacaaaaaaa
     1201 ccgcaatttg tggaacacat tgcggtttat cagcattcta agtattggtt gcgggggccg
     1261 gatttgaacc gacgaccttc gggttatgag cccgacgagc taccaggctg ctccaccccg
     1321 cgtccgtgga tgcgcactat actccgctcg cgttttcatg caaccttttt tttaccccga
     1381 tcacggattt agcatgattt tgaacaaaaa caaaccacta aagcctattt tttgtgcaaa
     1441 atttgcgtca gggagaggct gctcatttca ttaaacgcgg cggtttgtta tcttcgttgc
     1501 gccttatttt ttaacctgaa gaagagaaca atgaaaggac gttgggtaaa gtaccttctt
     1561 atgggcacgg ttgtggcaat gcttgccgcc tgctcttcca aatcaaccga tcgcggacag
     1621 caatataaag acgggaaatt tacccagcct ttctctctgg tgaaccagcc agatgccgtt
     1681 ggcgcgccga ttaacgccgg tgattttgcc gagcaaatta accatatccg taattcgtca
     1741 ccgcgtctgt atggcaacca gagtaatgtt tataacgcgg tgcaagagtg gctgcgcgca
     1801 ggcggtgata cccgcaatat gcgccagttc ggcattgatg cctggcagat ggaaggtgcc
     1861 gacaactatg gtaacgtgca gtttacaggc tattacacgc cggtaattca ggcgcgccat
     1921 acccgccagg gcgagttcca gtatcctatt taccgtatgc cgccaaaacg tggtcgtctg
     1981 ccgtctcgtg cggagatcta cgcgggggca ttgggcgata aatacatcct cgcttacagc
     2041 aactctctga tggataactt cattatggat gtgcagggta gtgggtatat cgactttggt
     2101 gatggcagtc cgcttaactt tttcagctat gcagggaaaa acggtcatgc ctatcgcagc
     2161 attggtaagg tgctgatcga ccgtggcgaa gtgaaaaaag aagatatgtc gatgcaggcg
     2221 attcgtcatt ggggcgaaac acacagtgaa gccgaggttc gcgagctgct ggaacagaac
     2281 ccgtctttcg tcttctttaa accgcaatct tttgcaccgg tgaaaggggc aagtgcggtg
     2341 ccgctggttg gccgtgcttc tgttgcctcc gatcgttcaa ttattccgcc aggtactacc
     2401 ttgctggcag aagtgccgct gctggataat aacggcaaat ttaacggtca gtacgaattg
     2461 cgtctgatgg tggcgctgga tgtcggtggc gcaatcaaag gccaacactt cgatatctat
     2521 caagggatcg ggccggaagc cggacatcgc gcaggttggt acaaccacta tggacgtgtc
     2581 tgggtgctga aaaccgcccc gggcgcaggt aacgtcttta gcggctgatg tgttattctg
     2641 tataccgttt ttacggatac gacagggtga gggataacct cacccttttt aattctgagg
     2701 tgctatgtct gtggtaatta gtgatgcatg gcgtcagcgt tttggtggca cagcgcgtct
     2761 gtatggtgaa aaagcgttgc aactgtttgc tgacgcgcat atttgtgtgg ttggtatcgg
     2821 tggtgtcggt tcctgggcgg cggaagcgct ggcgcgcacg gggattggcg caatcacgct
     2881 tatcgatatg gatgatgtgt gcgtcaccaa taccaatcgg caaattcacg ctctgcgcga
     2941 taacgtcggg ctggcaaaag cggaagtcat ggcggagcgc attcgccaga ttaacccgga
     3001 gtgccgtgta acggtggtgg atgatttcgt gacgccggat aacgtagcgc aatatatgag
     3061 tgccggttat tcgtacgtga ttgatgccat tgatagtgta cgacccaaag cggcgctgat
     3121 tgcttattgt cggcgcaata aaatcccgct ggtcacgacc ggtggcgcgg gtgggcagat
     3181 tgacccgacg cagattcagg ttaccgatct ggcgaaaacg attcaggacc cgctggcggc
     3241 gaagttgcgc gagcgcctga aaagcgattt tggcgtagtg aaaaacagta taggtaagct
     3301 cggcgtggat tgcgtgtttt ctactgaagc gctggtgtac ccgcagtcag acggtacggt
     3361 gtgtgcgatg aaagccacgg cagaagggcc gaagcggatg gattgtgcat ctggatttgg
     3421 cgcggcaacg atggtgaccg ccacctttgg gtttgttgcg gtttctcatg cgctgaagaa
     3481 gatgatggcg aaagcagcgc gtcagggtta aacaatcagg tcggatgcga cgcgaacgtc
     3541 ttatccgacc tggttctgtg cttaaacctg cttcgccgca gcgataattg cctcgcttaa
     3601 cgcattcaac ccctggctgc gtgaggcgct aagctgccca cgcaatccca gctcatcaaa
     3661 caatgccagc ggcgactgtg cctgtaattc ggcggcggtt ttcccctcaa cggcagtcaa
     3721 caacaccgcc aacaggccga gcacaatgcg cccttcgctg tcgccaaaga agtgcatttt
     3781 accgttttca gccacggtat atcccagcca gacgcggttt tcgcatccgg caatctcttt
     3841 agcctgcgct tttaactcgt ctggcaaagc cggaagctgt ttccccagca tgatcaactg
     3901 gcgatattta tcttcccatt gcgtcaacgg tgcgaaggta ttgcgtaacg tttctgcggt
     3961 tacggttgtg ccgaacggat gtccggcgaa ttgcgggttt gtcattaatc caccaataat
     4021 tccagcgcgc ggtcaacggc attcaccagc gcatccacat cactctttgt attatatggc
     4081 gcaaaagagg cgcgcagtgt gccggttacg cctaattctg ccagtagcgg ctgagcacaa
     4141 tgttgcccgg cccgcaaggc aataccgtac tccgccagta gcgtcaccat atcgctgtga
     4201 tgaacgccag caaaatcaaa ggccagcagg ctggaatcct ggcagcggaa tgaacgaaag
     4261 cctggacgtt tcgccagcgc atcttccgcc agcgttgcta agctatggct ccagttttcg
     4321 gcctggttga tatcgtaatc tgccagccat tccagcgccg cgcttaatcc tatgacacca
     4381 gccacatttg gcgttcctgc ttccagtttc cacggcgcag attgagtcgt gaagccgtca
     4441 aaactcactt cgtgaatcat tttgccgcca cccagccagg gcgacatcgc ttccagcagt
     4501 tctgatttgc catacagcac gccgataccc gtcgggccat acagtttgtg accagagaaa
     4561 gcatagaaat cgatatccag ttgctgaaca tccgcgggga aatgcaccgc cccctgagca
     4621 ccatcaacca tcaccaccat ccctgctgaa tgagcaaagg taatcgctcg cgccagatcc
     4681 gggcaaccgc cagtaacgtt cgacatctga ctcaacgcca gaatccgact acggggagta
     4741 atcagttctg gcaacaaatc gacatccggc agtcgctgcg cattaagcgg caatttcacc
     4801 actttggctc cagtttgttg ggcgaccatc agccagggga cgaggttggc gtggtgttct
     4861 gccacgctga caataatctc atcgcccggt tgcagacgcg gacgcgcata gcattgtgcc
     4921 accatgttga tggattcagt ggtgccgcgc gtccagacga tagttttatc atccggtgca
     4981 ttcagtaatt gcgccacttt ctctcgtgca gcttcataac gcgcggtcag gcgttgggct
     5041 tcggcaaact ggctgcgatg gacgtttccg gcgctcaggc tgtaaaactg tcgagtggct
     5101 tcaaccacgg cttcaggttt aagcgcggtc gcggcgctgt cgagatagac gcccgcatcc
     5161 tgtagtgcgg gaaactgggc gcgaaactgc gcgggattaa aaacgttcat ggtactcctc
     5221 ggcttgatgc accgatcgtg acgcaaaatt cactttgata caaggagagt gctaaccatc
     5281 agaattatct ggatatcctg gcgaagccgc gtaagtaaca tatgctgaat agtgttaggc
     5341 gttaatttta gcctgttaat caatactgtt aagatttatt aatcgcttta aggagaagat
     5401 gatgaaaaag actgccgcaa ttatttctgc ctgtatgctg acttttgccc tgagcgcctg
     5461 ttccggttcg aactatgtga tgcacaccaa tgacggacgt accatcgtct ctgacggcaa
     5521 accacagact gataacgata ccggtatgat ttcgtataaa gacgctaatg gcaacaaaca
     5581 gcagatcaac cgtactgacg tgaaagagat ggtcgaactg gatcagtaat tcgcgatcgc
     5641 aaggtaaaaa aaagcaccgc aattaggcgg tgctacatta atcactatgg acagacaggg
     5701 taaatgtaca ggaagtgaaa aaaggtagct ttgctaccat ggtctgaatc gcagaccaat
     5761 tgcaaacaca acaacacaac atcacaaccg taagccaaaa gttcaccaga acacgcattc
     5821 cgataaaact tttcgttccg gctcaggaag tgccgccact ataggtattt gctggtagaa
     5881 gctcaacgga caatttataa tggctcagat taaaaaaact aataggttac atagtgtgat
     5941 ctaattgtta aattcattta acatcaaagt ttaatagcca tgtctaaacg attaccaccg
     6001 ctaaatgcct tacgagtttt tgatgccgca gcacgccatt taagtttcac tcgcgcagca
     6061 gaagagcttt ttgtgaccca ggccgcagta agtcatcaaa tcaagtctct tgaggatttt
     6121 ttggggctaa aactgttccg ccgccgtaat cgttcactcc tgctgaccga ggaagggcaa
     6181 agctatttcc tcgatatcaa agagatattt tcgcaattaa ccgaagcgac gcgtaaactc
     6241 caggcccgta gcgccaaggg ggcgttgacg gtcagtttac tccccagttt cgccattcat
     6301 tggttggttc cgcgactttc cagctttaat tcagcttatc cgggaattga cgttcgaatc
     6361 caggcggttg atcgtcagga agataagctg gcggatgatg ttgatgtggc gatattttat
     6421 ggtcggggca actggccggg gctacgggtg gaaaaactgt acgccgaata tttattgccg
     6481 gtgtgttcgc cgctactgct gactggcgaa aaacccttga agacaccgga agatctggct
     6541 aaacatacgt tattacatga tgcttcgcgc cgtgactggc agacatatac ccgacagttg
     6601 gggttaaatc atatcaacgt tcagcaaggg ccaattttta gccatagcgc catggtgctg
     6661 caagcggcta tccacgggca gggagtggcg ctggcaaata acgtgatggc gcaatctgaa
     6721 atcgaggccg gacgtcttgt ttgcccgttt aatgatgttc tggtcagtaa aaacgctttt
     6781 tatctggttt gtcatgacag ccaggcagaa ctgggtaaaa tagccgcctt tcgccaatgg
     6841 atcctggcga aagccgctgc tgaacaagaa aaattccgct ttcgttatga acaataattt
     6901 acgtagggta cgaccatgac cagccgtttt atgctgattt tcgccgccat tagcggcttc
     6961 atttttgtgg ctctgggcgc ttttggcgcg catgtgttaa gtaaaaccat gggggccgtt
     7021 gagatgggct ggatccagac cggcctcgaa taccaggcgt ttcatacgct ggcgatctta
     7081 ggtctggcgg tggcaatgca gcgtcgcatc agtatctggt tttactggag tagcgttttc
     7141 ctcgcgttag gcacggtgtt gttcagcggc agcctttatt gcctggcgct gtcccatctg
     7201 cgtttgtggg cgtttgtcac tccggttggc ggcgtgagct tcctcgcggg ctgggcgtta
     7261 atgttagttg gtgctatccg tttaaagcgc aagggcgtaa gtcatgaata aggttgtatt
     7321 gctgtgccgt ccgggctttg aaaaagagtg cgccgcagaa attaccgata aagccggcca
     7381 gcgggaaatt ttcggttttg cccgcgtgaa agagaattcg ggttatgtca tttatgaatg
     7441 ttatcaacct gatgatggcg ataagttaat ccgtgagctg ccgttcagtt cattaatttt
     7501 tgcccgccag tggtttgtgg tgggggaact cctgcagcat ttgccgccag aagatcgtat
     7561 tacccccatt gtcggcatgt tacagggcgt agtagagaag ggcggtgaac tgcgtgttga
     7621 agttgccgat accaacgaaa gcaaagagtt gctgaaattc tgccgtaaat ttactgttcc
     7681 gctacgcgct gccttgcgcg atgccggggt gctggcgaac tatgaaacgc cgaagcgtcc
     7741 ggttgtgcat gtattcttta ttgcaccagg ctgctgctat accggttact catacagcaa
     7801 caataattcg ccgttctata tgggcattcc gcgcctgaaa tttccggcag aggcgccgag
     7861 tcgttccacg ctcaaactgg aagaggcatt tcatgtgttt attcctgcgg atgagtggga
     7921 tgaacgcctg gcgaacggga tgtgggcggt ggatttaggc gcttgccctg gcggctggac
     7981 ctaccaactg gtgaagcgca acatgtgggt ttattccgtc gacaacggcc cgatggcgca
     8041 aagtctgatg gataccgggc aggtgacgtg gctgcgggaa gacggtttca aattccgtcc
     8101 gacacgcagc aatatctcct ggatggtatg cgatatggtt gaaaaaccgg ctaaagttgc
     8161 ggcattgatg gcgcagtggc tggttaatgg ctggtgccgt gaaaccattt tcaatctcaa
     8221 actgccgatg aaaaaacgct acgaagaagt atcacacaat ctggcgtata ttcaggcaca
     8281 gcttgatgaa catggcataa atgctcagat tcaggcgcgg cagttgtatc acgatcgcga
     8341 agaagtgacg gtgcacgtcc gccgcatctg ggctgcggtg ggtggtcgtc gcgacgagcg
     8401 ataacaggca aggccggggc gtcgtcttcg gcctgctacc ctttccgtca ggctgttacc
     8461 aaagaagttg caacctggcg ctcatcagaa ataatgtgcg ttacctcatc cagatgcccg
     8521 atgcgggctt cccctgaacg attaaattta cttttatcaa tcaataacaa cgattgcgcg
     8581 gcacgtttta atagcatcga tttgtaatca gcgttgatcg cattggagtc ccacagtgcg
     8641 ccgctgctat cgatcccttc acaagaaaaa ataaacagat cgatttccag cgatttaagt
     8701 tgggaaatca gcgagggatt gacgtaacag ccatatttgc gctcaagtgt gccgccggaa
     8761 ctgatcagtt gaatgcgttc gcgtttaccg agttcatagc aaatcggatg gctattggtg
     8821 aagacctgaa tgttgatgtc aggcaactgg cgtgccagat accagcaagt tgaactggcg
     8881 tctaaggcta tcaccatccc ttcttcaatc cacgcgagcg cctcgcgcgc gatatctgct
     8941 ttatgcgcat aatggctttt cagcctgatg tgaaagggat cgccactgtc ttgattttga
     9001 cggtggatat atttagcgcg tccatgattg cgcagaattt taccctgcgt ctgtaattca
     9061 ttgagatcgc gacgaatggt ttctttactg acctttagct gttcagagag agcttccgtg
     9121 gtcaggctgg tatggttcag cagcaggtcg actatcgctt gctggcgtgc cgctttcata
     9181 tttttcaccc catcattcga taaccgcccg cgtcatgcgg gcgtaccggt atgagattac
     9241 ggtgttaccc ctttttttaa aagaatattc ccgtacttcg ctcgttcgcc tgtgataacg
     9301 atcgcaaagg ctttttgcgc ccgttcataa aacgcaaaac gattgatgcg gatgatgtca
     9361 ggacacgggg cttgtagtga aagcgcatta cggtaacgtc gttctacttc aggatcgaga
     9421 gtgtcacctt ctaccgccgc catcatcacc agcggcggtg cataactgtc cagttcaaat
     9481 aacgggataa tcgcctggag caagtcgctc accaacaggc catcagcgcg gatcacctgc
     9541 ggtcccatcg aatgggcggg aaagtgagca tcggaaaaaa taatttcatc tccatgtccc
     9601 atctctgcca gcactttcaa tagttcggga gaaattaacg gcgaaattgt tttcagcatt
     9661 tcacacttcc tctataaatt caggttcagt ttgcgggtag aaataacggt actgataatg
     9721 aatctgtgcg cgggcttctt cagggctgtt aaattcccct acgccatacc aaccgaacag
     9781 cgcagctcct gcgacggtcg tttcggcgtc gtcgagaact tttaccggaa tatcaagcat
     9841 attggcttta atctgattcc acaatgtgtt gcgacttcct ccaccgacta acaataattc
     9901 agaggcctta aagtgcccga ttttttccag catctgtaga ttgcgctgta attgcgcagt
     9961 taacccttcc agcgccgcgc gatagaaatg cccccgcgtg gtattaagcg tcactccttg
    10021 ccagccagcg ttctgacacg acaataaatc acactgcatt tttacgccat ccgcgctagg
    10081 cgcgataaga cgagcttctt caatcaacat ttgccagggt gtttcagccg tccagaacag
    10141 ttttctcacc cattccagca cgccggatgc cagccattgc atacctgggt tatacaaccc
    10201 tgcctggcta tccagttcgc aggtggaacc ggcgtactga cttaacagcg aagtatcaac
    10261 ctgggcgcta cggaccatta aaatttccca tgtaccggaa gagagcacgg gttcattttg
    10321 gtcggcacca gcgccaaaaa gggcgaactg ggtatcgtga cctgcggaaa tcaccggtat
    10381 gccaacgggt aagccgagca ttgctgcggc gctgttctgt agcgtaccaa tctgttcacc
    10441 cgcttccacc agacgaggga agagtcggcg tggaatacca gtggcttgta aaatttgcgg
    10501 actgaaatcg cgttgctgga tatccagcat ctggctggtt ccggccatcg tgatatcagt
    10561 agtgaattcg ccggttaaac ggtggttaat cagcgacgaa ataaagaacc aggcgtgcgt
    10621 gcgttccagc agttgtggat gattttcttt caaccacacc aacttatata acgtattgaa
    10681 actaaaggct ccgacgccag aaatagcctg caaccgctgt gcggagatca actgttcaat
    10741 attgtccata accgctgctg ttcgcggaca tttccagcta atgatcggat agagcagatt
    10801 gccttgctta tctaccagag cgccatccac accaaaggtg gtgacggcga taccgcggat
    10861 gtggcattca gtcagttcac tattgatttg ccgacagcaa tcagcaaagc gttgcaaaat
    10921 ggcgtctaaa gaccactggt gccaggtgtt gttttccatc gcgatatcgc tggcattagg
    10981 cgttgaggcg cgggcaacaa tttttccctg ccgattaacc gcgatggccc tgacattggt
    11041 cgcgccacag tcgagtacca ggataacttc ttgtttcata atcgctcctg caagacttca
    11101 atatccatgc cgtgcgcagc ccaggcagaa ggacgataca ctttggtctc ttcaacgttg
    11161 tgcatacata ccgggatacg cagcatggag gcgagagtga taaagtcagc gccaacgtgg
    11221 ccgatggtca gaaccccatg gttagcgccc cagttcgcca ttaccgagta cacatccgta
    11281 aacggccctt taccggtgag gcgcggtgca aaccaggtgg ttggccaggt tgagttggtg
    11341 cgtttgttga ggatgtcatg cacatccttc ggcaattcca cgctccagcc ttccgcgatt
    11401 tgcagtaccg gtcccaggcc tttgatgatg ttgacacgag tcatggtgaa cgggacgccg
    11461 ccttcggtaa ggaagcggga agagtaaccg ccgccacgga agtattcgtg gatcgccggg
    11521 caccattcgg tagcggcgag gcaagcgtca gcctcttgct gagagatttc ccagtgcggc
    11581 ttcatcgtcg ggttaccttc gctgtcgcgt tgtttacagg aaccgtccag cgcagcagaa
    11641 cctgagttga tcaaatggat gatgccgtgt tctgccagtc catcgagttt atgccccgtt
    11701 acacgctcaa ttgcttctgg tgaccagtag gtacgcacat cggcaaatac ctgagcggtg
    11761 ccggtgagct ggtgacccat tagcattgcc acgccgttaa gactgtcgtt ttcggtcgcc
    11821 acgacaaagg gttcgcgcac gccattccag tcaaatgaac tgttgaggat cgcttcggcg
    11881 gtgtcaccat tgggatattg atcggtccag tgacgttgcc cctggaagcc cgcagcgatg
    11941 gcgttgtagc caagtgattc ttccacgcga ccaatatcgg ccagtttgct gttgccttgc
    12001 atcatgtcgc ggatacacat cgccatcagt aaactttcgc gcagaactgc gcggctttgc
    12061 tcggcattgc gttgatactg tttgttattt tcatcttcgc catagcggaa gtttttatca
    12121 gcccaggcca gtgccatttc cagttcggct tcgtcgtaaa tcttctgatc gatacggcga
    12181 cgcagttcgg tcatatccac cgcctggact ttcattccca gccaggattc aaagaagttg
    12241 tgatcaacga tggaaccggc gatacccatc gaaacgccgc ccagcgacag atagctttta
    12301 cctttcatgc tggcgacggc caaaccggcg cgggcaaagc gcagcagttt ttcttcaaca
    12361 tcggcaggaa tcgatgtgtc atcggcatcc tgaacgtcat gaccgtaaat ggagaatgcc
    12421 gggatgcctt tctggctgtg agctgccaga gccgctgcca ggtaaacagc gccgggacgt
    12481 tcagtgccgt taaagcccca aatggctttc gggcgggttg gatccatgtc gatggtttca
    12541 ctgccatagc accagcaagg cgttaccgta atggtgaggc ctacattctg gctgctgaat
    12601 ttttcttcgc aagcagcggc ttcagccata cccgcgatac aggtatcgga aatgacacac
    12661 tcgacggcag ctccgcaggc atggcgcagt ttctcggtca gcagtgcggc cgtagctttc
    12721 gccatattca ttgtttgttc ttcaagcgac tcacgaacac ccatgcgacg accgtcaata
    12781 accgggcgga taccaatttt cggtaagctg atttttttca ttacagattc ctcactttat
    12841 tcggaaaata tgttcagtta gttgccgttt gagagcggaa acgggcaaag ataaagatga
    12901 ccgcgaagca gagtgcgggg atcagttcag cagtggggat gttgcccgcc gcgtcactga
    12961 caaaacccat gaccggagtg acaataccgc cgccaataat ggtcataacg atgaaggacg
    13021 aaccgtattt ggtgtcctgg ccgagattct taatgcccag cgagaagatt gttgggtact
    13081 gaatcgacat aaaggcgctg cataaagtca gggctattaa gcccacatga ccgccagcga
    13141 aggctgagat caggcacagt gccatagcga ttaatgcgta ggcggccagg actttgtgtg
    13201 gtgcgaagcg actgatgagc caggtaccgg tgaaacgacc aataaagaag cacaccatgg
    13261 ttccggttaa atagttagcg gcaaagcctg cagtcatacc tggaatttct tctacagcgt
    13321 agcgaatcaa atagctccag caggccgttt gtgcgccaac gtagcagaat tgcgctaata
    13381 ctgcccagcg ccagtggcga atacgcgcca ggcgagaaag cgatgcggag aacgatcctt
    13441 gtttggcgtc actgtgatta tcactctgca atgccgggaa tttcgtcagc atgatcagca
    13501 gggcgaccag taacacgata gccacgatga tcatataagg tgtctgtacc gataatacca
    13561 ggctgtgttt atacgcactc aattgctctg gagacatttt atcgagaacg tcttgcgatt
    13621 gatgtggcac gttagacaaa ataagacttt gcccaaagac aaccgcgata attgcgccaa
    13681 acgagttaaa tgtttgcgca agatttaagc ggaagtgacc actgctttcc ggccctaata
    13741 ccgtaacaaa agggtttgcg gcagtttcca gacaacctaa tccggctgca ataataaata
    13801 ggccaactaa aaataaggtg tagttcatta tttctgcagc gggccagaat aatgcagcgc
    13861 ccagggcata taaaaataac ccagtaataa
//
LOCUS       DAZPEB010000104        13846 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZPEB010000104 DAZPEB010000000
VERSION     DAZPEB010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13846
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.105"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(140..2075)
                     /locus_tag="ACHURJ_002620"
                     /pseudo
     CDS             complement(140..2075)
                     /locus_tag="ACHURJ_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017264.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
     gene            complement(2237..2320)
                     /gene="yqgH"
                     /locus_tag="ACHURJ_002621"
     CDS             complement(2237..2320)
                     /gene="yqgH"
                     /locus_tag="ACHURJ_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HFR1281512.1"
                     /translation="MHATSLHSLAYRRFARNVSLSMLLLMR"
     gene            2473..3171
                     /locus_tag="ACHURJ_002622"
     CDS             2473..3171
                     /locus_tag="ACHURJ_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFR1281513.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRISMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPTHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWFA"
     gene            3277..3352
                     /locus_tag="ACHURJ_002623"
     tRNA            3277..3352
                     /locus_tag="ACHURJ_002623"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3310..3312,aa:Phe,seq:gaa)
     gene            complement(3501..4037)
                     /gene="yghD"
                     /locus_tag="ACHURJ_002624"
     CDS             complement(3501..4037)
                     /gene="yghD"
                     /locus_tag="ACHURJ_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HFR1281514.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLAQHLAGRSPREKGMLLAAV
                     VFLFSVGYYVLIWQPLSERIVQQETMLQQLVAMNTRLRSAAPDIIAARKSATTTPAQV
                     SRVISDSASAHSVVIKRIAERGENIQVWIDPVVFNDLLNWLNALDEKYALRVTQIDAS
                     AAEKTGMVNVQRLEFGRG"
     gene            complement(4039..5217)
                     /gene="gspL"
                     /locus_tag="ACHURJ_002625"
     CDS             complement(4039..5217)
                     /gene="gspL"
                     /locus_tag="ACHURJ_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HFR1281515.1"
                     /translation="MSSILEIFFPLCAADPIHWQRRTPDVEHGIWSDVANEQLQQWLQ
                     TDAIRLYIPGEWISVWQVELPDVARKQIPTILPALLEEELNQDIDELHFAPLNIDQQL
                     ATVAVIHQQHMRNIAQWLQENGITRATVAPDWMSIPCGVMAGDAQRVICRIDECRGWS
                     AGRALAPVMFRAQLNEQDLPLSLTVVGIAPEKLSAWAGADAERLTVTALPAVTTYGEP
                     EGNLLTGPWQPRVSYRKQWARWRVMILPILLILVALAVERGVTLWSVSEQVAQSRTQA
                     EEQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTRLLAELSAIASTLKSASLSDIEM
                     RGFTFDQKRQILHLQLRAANFASFDKLRSALATDYVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            complement(5214..6191)
                     /gene="gspK"
                     /locus_tag="ACHURJ_002626"
     CDS             complement(5214..6191)
                     /gene="gspK"
                     /locus_tag="ACHURJ_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HFR1281516.1"
                     /translation="MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLSLKNEKRVHLAQPWASGPRFFPLPQGQIAVTLRDAQ
                     ACFNLNALAQPTTASRPLAVQQLIALISRLDVPAYRAELIAESLWEFIDEDRSVQTRL
                     GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMIRQQININ
                     TLDVTQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQL
                     KTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            complement(6194..6793)
                     /gene="gspJ"
                     /locus_tag="ACHURJ_002627"
     CDS             complement(6194..6793)
                     /gene="gspJ"
                     /locus_tag="ACHURJ_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001691118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HFR1281517.1"
                     /translation="MRRTRAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNSAVADH
                     DQKLNLMQQTMSFLTHDLTQMMPRPVRGDQGQREPALLAGAGVLASESEGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHDGYLERLAWPLTDAAGSVKPTMQKLIPADSLRLQFYDG
                     TRWQESWSSVQAIPVAVRMTLHSPQWGEIERIWLLRGPQ"
     gene            complement(6790..7161)
                     /gene="gspI"
                     /locus_tag="ACHURJ_002628"
     CDS             complement(6790..7161)
                     /gene="gspI"
                     /locus_tag="ACHURJ_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HFR1281518.1"
                     /translation="MKRGFTLLEVMLVLAIFALAATAVLQIASGALSNQHVLEEKTVA
                     GWVAENQTALLYLMTREQRAVRHQGESDMAGSRWYWRTIPLNTGNALLQAVDIEVSRH
                     EDFSSVIQSRRAWFSAVGGQQ"
     gene            complement(7221..7721)
                     /gene="gspH"
                     /locus_tag="ACHURJ_002629"
     CDS             complement(7221..7721)
                     /gene="gspH"
                     /locus_tag="ACHURJ_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001115132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HFR1281519.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASAGVVQTFATDSEPPAKKAAQDF
                     LTRFAQFKDRAVIEGQTLGVLIDPPGYQFMQRRQGQWLPVSATRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALELQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPATPFTLRFYS
                     AAQNAC"
     gene            complement(7725..8180)
                     /gene="gspG"
                     /locus_tag="ACHURJ_002630"
     CDS             complement(7725..8180)
                     /gene="gspG"
                     /locus_tag="ACHURJ_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HFR1281520.1"
                     /translation="MNSLSRTHKPRAGFTLLEVMVVIVILGVLASLVVPNLLGNKEKA
                     NRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADSRNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            complement(8197..9420)
                     /gene="gspF"
                     /locus_tag="ACHURJ_002631"
     CDS             complement(8197..9420)
                     /gene="gspF"
                     /locus_tag="ACHURJ_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HFR1281521.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKELIPVHIEA
                     RMNASAGGMLQRRRHAHRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
                     RQRLKSRLLQAMLYPLVMLVVATGVVTILLTAVVPKIIEQFDHLGHALPSSTRALIAM
                     SDALQASGVYWLAGLLALLVLGQWLLKNPTMRLRWDKTLLRLPVTGRVARGLNTARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRAALAELRLFPP
                     MMLYIIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            complement(9420..10912)
                     /gene="gspE"
                     /locus_tag="ACHURJ_002632"
                     /pseudo
     CDS             complement(9420..10912)
                     /gene="gspE"
                     /locus_tag="ACHURJ_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
     gene            complement(10912..12972)
                     /gene="gspD"
                     /locus_tag="ACHURJ_002633"
                     /pseudo
     CDS             complement(10912..12972)
                     /gene="gspD"
                     /locus_tag="ACHURJ_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
     gene            complement(13002..13846)
                     /gene="gspC"
                     /locus_tag="ACHURJ_002634"
                     /pseudo
     CDS             complement(13002..13846)
                     /gene="gspC"
                     /locus_tag="ACHURJ_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051199.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
BASE COUNT         3118 a         3981 c         3570 g         3177 t
ORIGIN      
        1 gttagcgctt ggcgacggcg gaacaaagct gattcgcgac gtaagaaagt ggcaggctca
       61 tatgacggac ctctgtaatg cggtatccaa cccgcgaata tcagcgtgat tcaccgtcgg
      121 atttactgct acgtcgccct cagggaatta aacctaaaaa gaaaatcgtc aaaattagag
      181 attccggttt gacacggaaa gtcatatcag cgctatgcgc atcctggggg ttcatcacca
      241 ctgacgggaa actctgctgg cggaacatcg ctttttgtag gtctttgacg tcgaaactga
      301 catacagatc gtgcatctcc tgacacaact ggcgcaaagt gtagtcgcga tagcgcaccg
      361 gatatttgtt ataaacgctc ggcaacacct cagccagcgg cgagtcatcc tcaatatgct
      421 gttcaaactg cgccaacatc tctaccagtt gtgccagctt ctcgtggctt tccgccggag
      481 ttaataagaa cagaatggag ttgagatcgc acttctccgg cacaatgccg ttctcacgca
      541 gatagtgcgc cagaatcgtc gccggaacgc caaagtcgct atattcgccg gtttcggcat
      601 tgatacctgg tgtagtgagt aacagcttgc acggatcaac aaaatactga tccgcggcat
      661 atccttcaaa gccgtgccac ttcgcccccg gctcaaaact gaaaaaacgg cggtcgctgg
      721 ctaacactga tgtcggataa tcctgccaca atttgccatc aacaacgggc gggataaacg
      781 ggcggaacag cttacagcgc gcaagaatag ccttgcgcga ctcaataccc aactcaacgc
      841 actcagccca cagccgacgc ccactctccc cttcatgaat tttggcgtta acatccagtg
      901 cagcaaacag cggatagaaa gggctggtag aagcatggag cataaaggcg ttattcaacc
      961 gcttatgcgg gcaaaaacgc gcctgtccgc ggatatggtt atctttttta tggatctgcg
     1021 acgtctgtga gaatcccgcc tgctgtttgt gcaccgactg agtcacaaag atccccggat
     1081 cgttttcgtt aagttctaat agcagcggcg agctatccgc catcatcggg ataaactgtt
     1141 cgtaaccgac ccacgcggaa tcaaacagaa tgtaatcaca cagatgccca acggtatcga
     1201 tcacctgacg ggcgttatag acagttccgt cataggttcc cagctgaata atcgccaggc
     1261 gaaacgggcg cggcagctcg gctttttctg gcgcaacgtc gcgaatttgc tggcgcagat
     1321 actcttcatt aaaacagtgc gcatcaatac cgccaatgaa accaaacggg ttgcgtgaag
     1381 cttccagata gaccggcgtc gcccccgcct gaatcagcgc gccgtgatga ttcgacttat
     1441 ggttgttacg gtcgaagagc accagatcgc cacgcgttaa cagcgcattc gtcaccactt
     1501 tattcgctgc cgatgtgccg ttcagcacaa aataggtttt atcggcatga aagactttgg
     1561 ctgcgaattt ctgcgcatct ttcgccaata agcagatcgc ccaattttac gtcagcgtta
     1621 cacatatcgg cgcgaaagac gttctcacca aagaaatcgt aaaaatggcg tccggcagga
     1681 tgctttttaa aaaacgcacc atgttgatgt ccagggcaag caaaggtgct gttgcccatc
     1741 tcaacgtact gcgtcagcgt gtcataaaac ggtggcagca aattctcttc atactgacag
     1801 gctgcggatt ccagctccag ccactgctgc tcgttgccgt tgattaccgc cgtaacgccc
     1861 gcaggtaatt caacagcatg ttcggaatac aaaaacaccg gtagatgaaa accggtgcgc
     1921 ttaagcaacg caagaatgcc actgcgacta tccgcagcgg taatgacgac tgccacgacg
     1981 tccgtaaaat cagtatctcc caacgccacc acgcgacgat gagaagaaag tcgggatacc
     2041 agttcactac tggcggcaat attcattgat ttcataagcg caaacccgtt tcggggaagt
     2101 aagaataccg gacaaggtgg aaaaccctgc cccatgagat atgggtcaaa ctggtcacca
     2161 gctccgaccg ccagacatca gtaaaagcag aaacgctctg attttactgt tgtcctgcag
     2221 tgagcgtgcg ttaacttcac cgcatgagca gtaacataga aagggaaacg tttcgcgcga
     2281 aacggcgata agcgagagaa tgtaaggagg tggcgtgcat cgggcaaact ccgtacagag
     2341 aggagaaaat tcgcgcaatc atggcacctt tcgctaaagc gtgcaagcca ggactttgcg
     2401 accagacaag ccatcaacaa ggcaaaaagt tcataataag aaaatcaaac aatacagtta
     2461 acaggaactc ttgtggtcat cggccctttt atcaacgcta gtgcagtctt actgggtggc
     2521 gttctcggcg cactgctcag ccaacgctta ccggaacgta tccgcatctc catgacatct
     2581 atttttggtc tggcatcgct ggggattggt attttactgg ttgtgaaatg cgccaacctt
     2641 ccagcgatgg ttttagctac gctacttggc gctctaattg gcgaaatttg tttgctggag
     2701 aaaggtgtca atacagcagt cgccaaagca caaaatctgt ttcgtcactc acgtaagaag
     2761 ccaacgcatg aatcttttat tcagaattat gtcgcgatta ttgtcctgtt ttgcgccagc
     2821 ggcaccggga tcttcggggc gatgaacgaa gggatgaccg gcgatccgag tattttaatc
     2881 gccaagtcat ttcttgattt ctttacggcg atgatcttcg cctgctcgct gggtattgcg
     2941 gtgtcggtaa ttagtatccc attactgatc atccagttaa cgctggcgtg ggctgccgcg
     3001 ctgatattac cgctgaccac accgtcgatg atggcagact tcagcgccgt aggcggttta
     3061 ttgctgctgg caaccggatt acgcatctgt ggcattaaaa tgttcccggt ggtcaacatg
     3121 cttccagcac tccttctggc aatgccgctt tccgccgcct ggtttgcctg acaatgcgtg
     3181 caatagcggc aaagtgatga tagattgtgc agtctgcagt aaattgaaga aatttgattg
     3241 acgagacgag gcgaatcagg tttaatgcgc cccgttgccc ggatagctca gtcggtagag
     3301 caggggattg aaaatccccg tgtccttggt tcgattccga gtccgggcac cactaattct
     3361 taagaacccg cccacaaggc gggtttttgc ttttggatct gacaataacc ttcacgaaaa
     3421 aagtttgctt ataaagtctg ggggaattac tctcgccacg ttaacgagag taattttatt
     3481 gatactaatc tcctgatact ttacccccgt ccaaactcca gccgctggac attcaccatc
     3541 ccagtcttct cagcagcact ggcatcaatt tgtgtcaccc gcagcgcata cttttcatcc
     3601 agcgcgttta accagttcag caggtcatta aacaccaccg gatctatcca gacctgaata
     3661 ttctccccac gctcggctat ccgcttgatg actaccgagt gcgcggaagc actgtcactg
     3721 ataacccgcg atacctgcgc aggcgttgtt gtggcggatt ttcgcgccgc aataatatcc
     3781 ggcgcggcgc tcctcagtct ggtgttcatc gccaccagtt gctgcaacat tgtctcctgt
     3841 tgtactatcc gttcgctcaa cggctgccag atgaggacgt aatatccgac gctaaacagg
     3901 aacaccaccg ctgccagtaa catgcccttt tcacgcggag aacgccccgc caggtgttgt
     3961 gccagccagt gttcgccacg gcttaactgg cgttcacgcc attgctgaaa atagtgaata
     4021 aatttatcgc gtaacatgtt atttcctccg caacgttacg ccgccggaaa ccgcatcacc
     4081 ctctttctgt aacgcgtcct gttgcacaac ataatcggtt gccagtgcac tacgcagttt
     4141 gtcgaaactg gcaaagttcg cggcccgtag ctggaggtga agtatctggc gtttttgatc
     4201 aaaggtaaaa ccacgcattt cgatgtcgga aagtgacgct gatttcaggg tgctggctat
     4261 cgctgacaac tcggcgagca gccgggtatc gtcggcctgt gggcgatatt ttttcagcgc
     4321 catcgtcacc tgagagcgta aattcacaat ccgcttctgc tccgggaaca acgttaagaa
     4381 ctgttcctcc gcctgggtgc ggctttgcgc cacctgttcg ctgacgctcc ataacgtcac
     4441 gccccgctcc actgccagcg caactagaat cagcaatatc ggcagaatca tcacccgcca
     4501 gcgcgcccac tgttttcggt agctgacgcg aggctgccac ggccctgtca gcaggttccc
     4561 ttccggttcg ccataagtgg taacggcggg cagagctgta acggtcaggc gttcagcgtc
     4621 tgcgccagcc catgctgata gcttttccgg tgcaatgccg actacggtta gcgaaagcgg
     4681 taaatcctgc tcattgagct gggcgcggaa catgaccgga gccagcgccc gcccggcgct
     4741 ccatccccgg cattcatcaa tgcggcagat aacccgttgc gcatcgccag ccataacccc
     4801 acaaggaatg gacatccagt ctggcgcgac ggtagcgcgg gtgatgccgt tttcctgcaa
     4861 ccactgcgca atgttgcgca tatgctgttg gtgaatcact gctacggttg ccagttgctg
     4921 gtcgatattc aacggggcga aatgcagttc atcgatatcc tggttcagct cttcttccag
     4981 taaggcgggc agaatggtcg gtatctgctt acgggcgaca tcaggcagtt caacctgcca
     5041 gacgctgatc cattcgccgg gaatgtagag tcgaatcgca tcggtttgca gccattgctg
     5101 gagctgttcg ttagcaacgt cagaccagat accgtgctcc acatcgggtg tacggcgctg
     5161 ccaatggatg ggatcagcgg cgcaaagcgg gaaaaaaatc tcaaggatgg aactcactca
     5221 ctttctcctg tctgatgcca gagaaccgaa aagtgttgtg ggcccatgcg gacaattaac
     5281 gagttcatcg tcagttcaat ctcattcacg gtgatatctg aacgcagcca gaagtaattg
     5341 ctgtccacgc tcaggacggt ttttagctgt tttttagtac gctcatcgac gtcagcaagt
     5401 agcggctgtg caagaaactg atcgacatct tcccagccct tcgccggacg ttgttgtaat
     5461 aacgcccgcg cctgaacggg gcttaaccac gggtcaaaca gcgcctcaag aatcacactt
     5521 tgcgtgacgt ctaaagtatt gatgttgatt tgctggcgga tcatcggcag cgcacagacc
     5581 agcggtttca gtttttgata aagcccggcg tccattccct gtaccacgcg catctcactg
     5641 atatcagcca gcggttgatt ggcggcgtag aacggcaccg aacgggcgag atactcgctg
     5701 tcttcacggc ccagacgtgt ctgcacgctg cggtcctcgt caataaactc ccacaggctt
     5761 tcggctatca gttcggcccg ataagcaggc acatccaggc gcgagatcag ggcaatcaac
     5821 tgttgtaccg caagcggacg cgacgccgtt gtcggctgag caagggcatt caggttaaag
     5881 caagcctgtg cgtcacgcag ggtgaccgca atttgcccct gcggcagagg gaagaaacga
     5941 gggccggatg cccacggctg tgccagatgc acgcgctttt catttttcag gctcagactg
     6001 agcgcgctca gcgccaggct ttccgcactg gcgctgtacc acagcgcctg ctggtactcc
     6061 tgctggctgc gcgttcgccc aagttgttgc tgcattcgcc cggaaagcgt gatggttacc
     6121 agcatcataa ccgccagcaa taccagcacc acgaccagtg ccattccgcg ttttggtggt
     6181 gaggtgatca tgattattgc ggcccgcgta acaaccagat gcgttcaatc tcgccccatt
     6241 gcggcgaatg cagggtcatg cgtaccgcca cagggatcgc ctgtactgac gaccagctct
     6301 cctgccagcg cgtgccgtcg tagaactgca aacggagcga atcggccgga atcaactttt
     6361 gcattgtcgg cttcacgctg cctgcggcat cggtcagcgg ccaggctaac cgttcgagat
     6421 aaccgtcatg aatgcggtaa ccgacggtga gcagattgct gcgcggcaga cgcatcaacg
     6481 ggttaaccac accgccgcgc acaaaacgca ttccttcact ctcagacgcc agcacgccag
     6541 cgccagccag taaagctggt tcacgctggc cctgatcgcc tcttaccgga cgcggcatca
     6601 tttgggtcag atcgtgggtc agaaaactca tcgtttgctg catgaggttt agtttttgat
     6661 cgtgatcggc gacggcgcta ttcacgcgcg tgacgccgtt agtcacctgc tgcgccatca
     6721 gtgccagcga ggcaaaaatg gcaattgcca ccaacatttc cagtaacgtg aaaccagcgc
     6781 gagtccttct cactgttggc ctcccacggc gctaaaccag gcgcgtcgtg actgaatcac
     6841 cgacgaaaag tcttcgtgac ggctgacttc aatatccacc gcctggagca gcgcgttgcc
     6901 tgtattcaat gggattgttc gccagtacca gcggcttcct gccatatcgc tctcgccctg
     6961 atgcctgacc gcccgctgct cacgggtcat caggtagagc agcgcagtct ggttttcagc
     7021 tacccagccc gctaccgttt tttcctcaag aacgtgctga ttactcagcg caccgctggc
     7081 aatctgtaac accgccgtgg cagccagcgc aaaaatcgcc agcacgagca tcacttccag
     7141 caaggtaaat ccgcgcttca tggcatcctc tcatcacatt gattgaggga taacgcgcca
     7201 tcgtgtgcca gctttaccgc tcaacatgcg ttttgcgccg ccgagtagaa gcgcagcgta
     7261 aacggcgtgg cgggttcaaa aggcgaaaaa cggatctgcg gcgtcttctt tttcgcctct
     7321 ttttgcaact ccagttcaat atcgtgcagc gtcaggcgac gacgttgcag ctccagcgca
     7381 tattccttct gccagatatc acttccgggt tgtaacagca tctgcacctg tttcggcacc
     7441 gtaacctgtg ccgataaccg ggtcgcagaa acgggcaacc actgcccctg acgacgctgc
     7501 ataaactgat agccaggcgg gtcgattagc acaccgagtg tctgtccttc aatcactgcc
     7561 ctgtccttaa actgcgcaaa gcgagtcaga aaatcctgtg ccgctttttt cgcgggcggt
     7621 tcagaatcgg tcgcaaacgt ctgtaccacg cccgcactgg caaggccgat aaggaaaatc
     7681 accagcatga tttccagaag tgtgaatccg cgttcaggca ctgcttactg aaactcctgc
     7741 aaattccagt taccgatatc tgcgccagcg ccttccccgt tttcctgacc atccgccccc
     7801 agggtataaa catcaaacag tcctttttca cccgggctga gatactgata atcattgccc
     7861 cacggatcct ttggcagtcg tttgatgtat ccaccagtac ggtagttgcg ggagtccgcc
     7921 atattggctg gctgctggat cagcgcttcc agcccctgtt ctgtggtcgg ataacgcccg
     7981 ttatccagtc ggtacatatc tagtgcgttt tccagcgcca cgatatcgct gatggctttt
     8041 tgccgattgg ctttctcttt gttgcccaac aggttaggca ccaccagact tgccaggacg
     8101 ccaagaataa caatcaccac catcacttcc agcagggtaa aacctgcccg tggtttgtgt
     8161 gtgcgggata acgaattcat gtgataactc cgtaaattac attccaacca tattattcag
     8221 ttgcagcatc ggctcgagga tggcgatgac aataaacagc accacgcccg ccatcatcac
     8281 caccagcgca ggctcaaaca gccccaacgc cagccccacc tgggtatcaa attcccgttc
     8341 ctggttaaca gcggcctgct caagcatggt ttccagttcg ccgctctgtt cgccggaagc
     8401 gataatgtac agcatcatcg gcgggaacag gcgcaactcc gccagcgcgg cgcgcagact
     8461 gcttccttcg cggacgcgat ctgccgccag cagcagttgt tgttcgacat agcgatttgc
     8521 cgacacggcg gcagcggtct gaatgccttc cagtagcgga acactgctgg cggtgaggat
     8581 gcttaatgtg cgggaaaaac gcgccgtatt cagcccgcgc gcaacacggc ccgtcacggg
     8641 cagacgcagc aaggttttat cccagcgcag gcgcatagta ggatttttaa gtagccattg
     8701 ccccagcacc agaagcgcca gcaatcccgc cagccagtaa acgccgctgg cctgtaacgc
     8761 atcactcata gcgataagcg cacgggtgga ggagggtagc gcgtgtccga gatgatcaaa
     8821 ctgttcgatg attttcggca ccactgccgt cagcaaaata gtgaccacgc ccgttgccac
     8881 caccagcata accagcggat agagcatggc ctgcaataga cgtgatttca ggcgctgtcg
     8941 ctgttcggtg taatctgcca ggcgattgag caccacgtcg agatgcccgg atttttctcc
     9001 ggcagcgacc atcgaacaaa acagggaatc aaagacgcgg ggatgttcgc gcaggctgtc
     9061 cgacagggta taaccttcct gaatccggct gcgcagcgcc attccgaggc tcttcacatg
     9121 cagtttttca ctttgctcac tgaccgcctg caagcaggtt tccaacggca ttgctgcctg
     9181 caccagcgtt gccagttggc gcgtgaacag cgccagatct gccgccgcca cgcgacgatg
     9241 tgcgtgccgc cgacgctgca acattccccc tgccgatgca ttcatcctgg cttcaatgtg
     9301 cacggggata agctctttac cgcgcaacaa ctggcgggca tgacgcgcgg aatccgcctc
     9361 aatcatgcct ttggttttgc gaccattacg ctccagcgcc tgatagtaaa acagtgccat
     9421 tacgcctcca tggttacccg cagaacttca tcgagagagg tttctccggc gagcactttc
     9481 tcaatgccgt tgctgcggat acccgcagag tgttgccgga cataacgttc cagctccagc
     9541 tccccggcct gacggtggat caaatcacgc aatgtggcat ccaccacgtc agctcatgga
     9601 tggcagtccg tccgcggaaa cctttgtgat tacaggcggg gcagccctgc ggatggtaca
     9661 gagtgacggt tcgcgcatcg gtaatgccca gcaggcgttt ttcttcgtca gtggcaggcg
     9721 cggcctgacg gcagtcggga cacagcgtgc ggaccagtcg ctgcgccatc acgcccgtca
     9781 gactggaaga gagcaggaaa ggctccacgc ccatatcctg caaacgcgtg atcgccccca
     9841 ccgccgtgtt ggtatgcagc gtggaaagga ccaggtgtcc ggtaagcgat gcctgaacag
     9901 cgatttctgc ggtttcggta tcgcggattt caccgaccat caccacatcc gggtcctgac
     9961 gcaaaatcgc gcgcaggcca cgggcgaagg tcatgccgac gcgggtgtta acctgtgtct
    10021 gaccgatccc ttcaatcatg tattcgatgg ggtcttcaac cgtgagaatg ttacgcgaat
    10081 ggttgttcag ctcctgcaat ccggcgtaca gcgtggtgct tttgccggaa cccgtcggcc
    10141 ccgtcaccag aaagatgccg tgcggtttgt gtaacagctg gcgcaactgc acggtcagtt
    10201 gctggctaag ccccagacgc tccagcgtca ggcgggcctg gtttttgtcc agcagtcgca
    10261 gcaccacgcg ctcgccccag gcagaaggca tggtggagac acgcacgtca atcgcccggc
    10321 cgcccagcag cagcgcaata cgtccatcct gcggtacgcg cttttcggcg atatccagcc
    10381 gtgccatcac cttgatacgc gataccagca gcgaggccag tttgcgcccc gggcgcagca
    10441 tttcatgtaa tgtgccgtca acgcgaaaac ggatcaccag actcttttca aacgtctcga
    10501 tgtggatatc cgaagcgcct tctttaatcg cctctgccag catggcgttg atcagtttga
    10561 tgatcggcgc atcgtcgtca ctttccagca gatcttccgt ttctggcagt tcttccgcga
    10621 gagtaaaaaa gtcctcagca gaaccgagat cttccatcag ctggcgagcc tcggaagagt
    10681 cccgctgcca gaccgcattc agccgctgtt caaattcggc ctcgtcgatt tgccgcagcg
    10741 taaagggcgc gttcagcccc cgctgcagct cctgcaaaac agagagcgat agcggatgaa
    10801 cgtggaggat ctccagcgac gcttcgcacc atgccaccag gctaaaccga cggctgaaac
    10861 tgtagggcag acgcacggtc ttggcggtgg tttcctgtgc tacaggcacc attaacgcgt
    10921 tctcccggca ttgaggaacg cgcgaacttc cggcggtaag gcctggtttt gcgctggcag
    10981 taccggctgt gcggtgtgcg gcatcaggct taaaccttgc tcatcacgat agatctgttc
    11041 ggcgcgcata tagttatatt tgcgctgcga cacgccgtct gccgccatac cgtcatgcag
    11101 aatggtcggg cggataaaca ccatcaggtt acgtttttct tttttatccg ccgtcgattt
    11161 aaacaggtta ccaatcaatg ggatatcgcc cagcagaggc actttcgcca cgctttctcc
    11221 cgcctgatcg tccatcagac caccaagcac aatcagctca ccatcgttag ccagcacggt
    11281 ggttttcagt ttgcgctcac caaacaccac gtcgaggctg gtctgtcctt ccaccttcga
    11341 gacttcctgc tcaatcacca tctgtaccgc gttaccttcg ttaatctgcg gcgtgacttt
    11401 cagcatgatg ccgacttttt tcctttctac tgtattgaaa ggattgctgt tattggagcc
    11461 aacggtagag ccggttaata ccggaacgtc ctagcccacc atgaagaagg cttcctggtt
    11521 gtccagcgta gtgatgctcg gcgtggagag aacgttcgag ctggagtcgt ttttaaccgc
    11581 ctgcaccagt gccatccagt cgcctttcac cacgccaacc gccgtaccgc taaatccaga
    11641 aagaagctgg gcgagtgtgg agagatcgcc gttggtatcc ggattaatgg tggtagcgcc
    11701 gttttcactg atcaccgtcg agcctttctg cggtttcgcc tgagaaatgg ctgcccccag
    11761 cgtaccgata gggatctgcg taccattagc aaactgcatt aatccggcat ctttcgacgc
    11821 ccactgcacg ccgaagttga tattgctacc ttcggcaact tccacgatca acgcctcgac
    11881 gtgcacctga gcacggcgaa tatccagttg ttcaattacg ctttgcagcg actgcatgat
    11941 gtcctgcggc gcggttacaa tcagggcatt actgtgtttg ctggcggcga tggatacaac
    12001 ctcacgcccg ctaccgaccg tgccttccgc ctcttcttta gccgccgtga gcgtgccgct
    12061 gacctgtttc agcacatcga ccagatcttc ggctttgctg tatttgagat agaaaacctg
    12121 gctgttgccg ctgcgttcca tttctgagtc cagccgacgg atcaggcggc gcattttgtc
    12181 ccgcgtggcc gggtcaccac tgacaatcac actgttggtg cgttcgtcgg cgacaatttg
    12241 agatttcagc gtcgcaggct ggttctcgcc gctgtttttc gtcaggcttt ccagcacgcg
    12301 ggcgatttcc gaagccgagg cgttatccag cgggatcacc tcttcggtgc gattacccgc
    12361 gtgatccacg cgctggatca cttccgtcag gcgctccaca acggaggcgc gtccggtgag
    12421 cataatcacg ttggagggat cgtaattaac aacgttgcct gagcctgcgc tgtcgatcat
    12481 ctggcgcaga atcggtgcca gctcgcgtac cgacacatta cgtaccggca ctactttggt
    12541 gaccatttca tcgcccgcgt agttgtcgct gccttcaccg accagcggca gcggctcgac
    12601 tttcgcagcg ctggatttca ccaccttcag cacgtcgttt tccatcggta cgacggcata
    12661 cccctgtgct tccagcaggt taaggaatag ctggtaatac tggcgttcat tgagcggggt
    12721 catggtgcga atactcactt tcccctgtac gcccggcccc ataatgatgg ttttattaag
    12781 gttagcgccg acggtttcga tgaacgattt caggtcggta tctttaaaat tagcggtgaa
    12841 agtggcttct tccgcccaga ccggtgaact gcataatgcc gctgccagca ccagcggcag
    12901 taaacgcttt tttgttttga ggccagttgt cttcttacgc cagaccgaca acgtaatatc
    12961 acgccaaaac acgatgaatg attctcctgg attaaatgcg gttagcgcag cgcgatggaa
    13021 atgtcgtggc gcgcaccctt gcgtaaaacc gtaagttgaa tggaatccat tgaaggtaac
    13081 tgccgcatca gagcaatcat tgctcgtgga tcagtgaaat cctgctgatt tagcgcaatg
    13141 gcgatatcgc cttccttgaa accgctggca tcgaacagag aacgatctgc ccccggcttc
    13201 actgcataac cgacaattcc ctccttacgc acaggcgtaa gctggatata gttaaaaatt
    13261 ttctgcggat ctttcgccag tgcctgacgc acggcagtcg gtatctcaac tggcgcactg
    13321 acagcaggtt cagcaacagc ttgctttgct tcgtcactga cagctttttt gttggtcgcg
    13381 gcaacggtgg aacgctcctc ttcagccagg ctcaggcgct ctatttttcc ctgatagcgc
    13441 agcatcacat ggtcgcgatt gatttcctca atcaccgcgt tgtgcgagcc aagcgtttca
    13501 ccctgcaaat agacctgctg tttaccgcct tcttcaataa ccgcgccggg tctggcacca
    13561 aaggcgatcc cgcgcgcacc acattaagac gcgtttctgc cacaggcaca ggttcgggtt
    13621 gttttaccgg cgtggcgacg ggctgatatt tgccaaacca gttttgctgg ctgattaatt
    13681 gcacgtcatt tttatcgaac gtttttgcat ccgcacgggg cggtttattc gccgatgggg
    13741 aaacagccgt atattccgca gaaaaggaga aatagcgcca gagtgaatgc gccacttttg
    13801 cagaaataat aagcagcatc agccagaaca tcccgcgtac aatttt
//
LOCUS       DAZPEB010000105        13612 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZPEB010000105 DAZPEB010000000
VERSION     DAZPEB010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13612
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.106"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(94..1185)
                     /gene="ychF"
                     /locus_tag="ACHURJ_002635"
     CDS             complement(94..1185)
                     /gene="ychF"
                     /locus_tag="ACHURJ_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFR1281522.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1302..1886)
                     /gene="pth"
                     /locus_tag="ACHURJ_002636"
     CDS             complement(1302..1886)
                     /gene="pth"
                     /locus_tag="ACHURJ_002636"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFR1281523.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2164..2442
                     /gene="ychH"
                     /locus_tag="ACHURJ_002637"
     CDS             2164..2442
                     /gene="ychH"
                     /locus_tag="ACHURJ_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFR1281524.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2497..4176)
                     /gene="dauA"
                     /locus_tag="ACHURJ_002638"
     CDS             complement(2497..4176)
                     /gene="dauA"
                     /locus_tag="ACHURJ_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFR1281525.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVIIYPVSQQFGL
                     AGLLVAALLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPISGRLAFFPNRRAAMADL"
     gene            complement(4301..5248)
                     /gene="prs"
                     /locus_tag="ACHURJ_002639"
     CDS             complement(4301..5248)
                     /gene="prs"
                     /locus_tag="ACHURJ_002639"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFR1281526.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5399..6250)
                     /gene="ispE"
                     /locus_tag="ACHURJ_002640"
     CDS             complement(5399..6250)
                     /gene="ispE"
                     /locus_tag="ACHURJ_002640"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFR1281527.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(6250..6873)
                     /gene="lolB"
                     /locus_tag="ACHURJ_002641"
     CDS             complement(6250..6873)
                     /gene="lolB"
                     /locus_tag="ACHURJ_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFR1281528.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTHPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7087..8343
                     /gene="hemA"
                     /locus_tag="ACHURJ_002642"
     CDS             7087..8343
                     /gene="hemA"
                     /locus_tag="ACHURJ_002642"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFR1281529.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8385..9467
                     /gene="prfA"
                     /locus_tag="ACHURJ_002643"
     CDS             8385..9467
                     /gene="prfA"
                     /locus_tag="ACHURJ_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFR1281530.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELRKAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9467..10300
                     /gene="prmC"
                     /locus_tag="ACHURJ_002644"
     CDS             9467..10300
                     /gene="prmC"
                     /locus_tag="ACHURJ_002644"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFR1281531.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGGREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10297..10689
                     /gene="sirB2"
                     /locus_tag="ACHURJ_002645"
     CDS             10297..10689
                     /gene="sirB2"
                     /locus_tag="ACHURJ_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFR1281532.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            10693..11502
                     /gene="sirB1"
                     /locus_tag="ACHURJ_002646"
     CDS             10693..11502
                     /gene="sirB1"
                     /locus_tag="ACHURJ_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFR1281533.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDPVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            11538..12392
                     /gene="kdsA"
                     /locus_tag="ACHURJ_002647"
     CDS             11538..12392
                     /gene="kdsA"
                     /locus_tag="ACHURJ_002647"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFR1281534.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(12541..12648)
                     /locus_tag="ACHURJ_002648"
     CDS             complement(12541..12648)
                     /locus_tag="ACHURJ_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR1281535.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(13076..13183)
                     /locus_tag="ACHURJ_002649"
     CDS             complement(13076..13183)
                     /locus_tag="ACHURJ_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR1281536.1"
                     /translation="MTLAKFAMIFWHDLAAPILAGIITAAIVGWWHNRK"
BASE COUNT         3356 a         3475 c         3513 g         3268 t
ORIGIN      
        1 cggtgaaatg aaaaatccac gcaattgcgt ggattttata tacttttgcg ctcttcatga
       61 gatttagcga aacctcatga gacaataaat taattagacg ttgaaaagga agttcatcac
      121 atcgccatct ttcacgatgt aatctttacc ttctgcacgc attttgcctg cttctttcgc
      181 gccttgttca cctttgtaag tgatgaaatc ttcaaacgag atggtttgtg cacggataaa
      241 gcctttttca aaatcagtat ggattttgcc cgctgcctgc ggcgcggttg ctccaaccgg
      301 aatggtccat gcacgcactt ctttcacccc agcggtgaag taagtttgca ggttcagcag
      361 cttataaccg gcacggatca cacggttcag gcccggctct tccagcccaa gctcctgcat
      421 aaactcgtca cgttcttcgt cgtccagttc ggcaatgtct gcttcaacag cagcacaaac
      481 cggaaccaca acagaacctt ctttcgccgc gatttcacgc acctggtcaa gatatgggtt
      541 gttttcaaaa ccgtcttcgt tgacgttggc gatgtacatt gttggtttca gcgtcaggaa
      601 gctcaggtaa cgaatagccg ctttctcttc agcgcttaaa tccagcgcgc gcagcatacc
      661 tgcattttcc aactggggca ggcatttttc caggaccgcc agctcagctt tcgcgtcttt
      721 atcgccacct ttggctttct tctgtacgcg atgaatcgca cgttcgcagg tgtcgaggtc
      781 tgccagcgcc agttcggtgt tgataacttc aatatcgtca gccgggttaa ctttgccgga
      841 aacgtgaatg atgttgtcat tttcaaagca gcgaacaacg tgaccgatcg cttcggtttc
      901 acggatgttg gtcaggaact ggttacccag accttcgcct ttcgatgcgc cttttaccag
      961 accggcgata tcgacaaatt ccatggtcgt gggaagcgta cgctgcggtt ttacgatttc
     1021 agccagttga tccaggcgag gatcaggcat tggtacgacg cctgtgttcg gctcaatggt
     1081 gcagaatgga aagttggccg cttcaatacc ggctttggtc agcgcgttga acagggtaga
     1141 tttcccgacg ttgggcaaac cgacgatacc gcatttgaat cccatgattt aaatcacctt
     1201 aatatcttaa taatcaacct gttattgcta acagattgca gaaatggaaa taactttgcc
     1261 tattatacac ggcactcggc aaaaatgccg cagacaacga cttattgcgc tttaaaggcg
     1321 tgcaatcggt tcgttgcttt ggtcaagcca tctgtaaacc acatttcagt acaacgcgcc
     1381 gcttcgtcaa tggcttcatc aattaacttc tgttcactaa caggcggttt gcctaacaca
     1441 aaaccgacaa ctttattttt atcgcccgga tgaccgattc cgatgcgtaa acggtgaaag
     1501 ttagggttat tacccaattt actgatgatg tctttcagtc cattgtgacc accatggcca
     1561 ccgcccaatt taaatttggc gacgccagga ggcagatcca gttcgtcgtg ggccaccaga
     1621 atttcgtccg gattaatgcg gaaaaaactg gccatcgccg caacggcttt gccgctgaga
     1681 ttcataaatg tagtcgggac taacaggcgg acatcttcgc ctccaagagt gactcgcgaa
     1741 gtataaccaa agaatttagc ctcttcgcgc agcggagcgc gcaaacgctc tgccagtaag
     1801 tcaacgaacc aggcaccagc attatgtcgc gttgcggcgt attcagcacc ggggttcgcc
     1861 aggccgacaa tcaatttaat cgtcacgttt ttttgtcctg agtgtgtaca taactggcgc
     1921 gtagtttact ggttgcgacc ccgcttgaca aaaaactgcg tatcaaatgc agataacgta
     1981 ataattgcct gagtgggcta ttagaaagtc aaggtgttca ggcgtttatt tgtaaagttt
     2041 tgttgaaata agggttgtaa ttgtgatcac gcccgcacat aacccacagg gtgttgtcta
     2101 tactttacac ataaggaaga ggggtattcc ctgttacaac ccagaaagtt ccggaggtga
     2161 catatgaaac gcaaaaacgc ttcgttactc ggtaacgtgc tcatggggtt gggtctggtg
     2221 gtaatggtgg tcggcgtggg gtattcaatc ctcaaccagt taccacagtt taatatgccc
     2281 cagtatttcg cacatggtgc agtgctaagt attttcgtcg gtgccattct ctggctggcg
     2341 ggtgcccgtg ttggcgggca tgaacaggtg tgcgaccgtt actggtgggt tcgccactat
     2401 gacaaacgtt gccgccgtag cgataatcgc cgtcatagct aacaaataat gcagtttgct
     2461 gaccagtcag tttgcgctga ctggtcaatc tcgtacttat aaatccgcca tcgccgcgcg
     2521 acgattcggg aagaacgcga ggcgtcccga gattggttga atgcctgcgc gagccatagt
     2581 gcgcagtggc tggaattcca cgttgcacac gcgcagttca catccttcgg gcagacgctt
     2641 cacaaaacgc tggaacgcat caagaccacc agcatcaagt accggaacgg catcccactt
     2701 cagaatcaca atccgtttgc cttcaagacg tgactccagg tccgtgaaca agccttcagc
     2761 agcagcaaaa aacagcgggc caataacgcg cagtaccaga acatcgtctg gaacatctac
     2821 gactaccggt gccaggcgag tcatacgtgc gatacgacgc ataaacagca gcgatgccag
     2881 cacgatcccc acgctgatgg caataaccat atcaaacagc acggtcagcg acatgcacag
     2941 cagcatgaca atgatgtcat ctttcggtgc atgacgcagc aagtcgacca ctttatgcgc
     3001 ttcactcatg ttccacgcca ccatcaacag cagggctgcc atagcggaaa gcggcagcca
     3061 ggagagcagc ggtgccagta ccagcagggc aagaataacc agaatagagt ggatcaccgc
     3121 tgagataggg gaagttgccc cggcacggac gttagcggca gaacgcgcga tggcagctgt
     3181 agcggtaata ccaccaaaga atggagcgat aatattcccc agtccctgtc caaccagttc
     3241 gctgttcgcc ttgtgtttcg tcccggtcat accatccagt accacggcgc agagcagaga
     3301 ttcgattgcg ccgagcattg ccattgagaa tgccgcaggc agcagtgtgc gaatagaatc
     3361 ccaggttagc gtgaattctg aattaggcag atcccacggc agcaccagtt gcggcagcag
     3421 ttgcggaata ccgttaccct gagaaccatc ggccaggacg tagtggaatt gcgaaccgat
     3481 ggtagcaaca tgtccgccga gcaggttaac aatccccatc accgcgcaac cagccagcaa
     3541 ggccggaagg tgaccgggta aacgaatgcc cagacgcggc caaaaaacaa gaatacctag
     3601 cgtcacaatg ccaatggcag catcacccac attaatggtc ggcagcgcca taaataatgc
     3661 gccgactttt tgtagataat gttccgggac atgggccatt tgcagaccga gaaaatcttt
     3721 aatctgcatg gtaccgatgg tgatcccgat acccgaggtg aaacctaagg tgacggaaac
     3781 cggaatatac tcaatcaggc gaccaaagcg tgccagaccc atcagaatca aaaagatccc
     3841 cgacagcaag gccgcaacca gcagtcctgc cagtccaaac tgttgcgaca cgggatagat
     3901 aattaccaca aatgccgcag tcggaccgga aacgctaaag cgtgacccac ccgtcagagc
     3961 aatgacaatc cccgcaacag ctgcggtata taaaccgtac tggggtgcca caccactacc
     4021 aatagccaac gccatcgcca gcgggatagc aataatcccg acggttatcc cggcaatcag
     4081 gtcacgggta aaccgtgcgg cagtatattt ttctttccag caagcgtcga tcagagcgcg
     4141 gaaaggcatc acatgtgagg aaaatatttt gttcacaata atgtttcatc cgtgagcgca
     4201 tcatctgtca actaaatggc aggtgaagga ggcataggtc atacaaatgg atattacaga
     4261 caaaaaaacc cgccgcagcg ggtctttgag ccgggttcga ttagtgttcg aacatggcag
     4321 agatcgattc ttcgttgctg atacgacgaa tcgcttcggc cagcatacct gacagggtca
     4381 gagtacgcac gttcggcagt gatttgattt catcgctcag cggaatggta tcgcagacaa
     4441 cgacttcatc aattacagag ttacgcaggt tgttcgccgc gttgccagag aagatcgggt
     4501 gagtcgcgta cgcaaataca cgtttagcac cacgttcttt cagagcttca gcagctttac
     4561 acagcgtacc gccagtgtcg atcatatcat cgaccagtac gcagtcacga cctgcaacgt
     4621 caccgatgat atgcatcacc tgggaaacgt tcgcacgcgg acgacgtttg tcgatgattg
     4681 ccatatcggt atcgttcagc agcttagcga tagcgcgggc acgcacaacg ccgccgatgt
     4741 ccggagaaac cacaattggg ttatccagat tcagctgcag catgtcttcc agcaggatcg
     4801 ggctaccaaa tacgttatca accggaacgt cgaagaaacc ctgaatctgt tcagcgtgca
     4861 gatccactgt cagcacacgg tcaacaccga cgctggagag gaagtctgca accactttcg
     4921 cagtgattgg tacacgagcg gaacggacgc gacggtcctg gcgcgcatag ccaaagtagg
     4981 ggataacagc ggtgatacga cctgcggaag cacgacgcag ggcatcaacc ataacgacta
     5041 attccatcag gttgtcgtta gtaggggcac aagtggactg gatgatgaaa atatcaccac
     5101 cgcgtacatt ttcattaatt tgtacgctga cttcgccatc gctaaagcga cctacagcgg
     5161 cgtcgccgag tgaagtgtac aggcggttgg caatacgttg tgctagttcc ggggtggcgt
     5221 taccagcaaa aagcttcata tcaggcacga gaagaacctc aggcatgcgt ccattggtgg
     5281 aaagaatctg ccgaaaactg tgcgggccag gcatgatcct ttccaggcag tgtattaaag
     5341 agcgcgatgc aacgtctgga acaaggtgac gttgtcaccg aaactcagct tgcccggctt
     5401 aaagcatggc tctgtgcaat ggggaaagat taacgccttt cgccacaaag ccattgagcc
     5461 attccggggc ttgctctagc acctggcggg cttcagactc tgtatcaaat tcagcaaaga
     5521 cacaggcccc tgtcccagtc aggcgcgacg gggcgtattc taacagccag gaaagcaccg
     5581 catcaacctc gcgaaaacgt tttcttgcga taacctcgca atcattgctg aattcacatt
     5641 ttagcaacgt ttctattgac ctttttggcg tattgcgcgg gagttcagga tctttaaaaa
     5701 tcaccggagt cggaatactt acaccagggt gcgccaccag ataccacttc tctggcggat
     5761 ccaccggcgt tagtatttca ccaacgcctt cggcaaacgc ggcatgcccc cgaacaaaga
     5821 caggaacatc tgcgcccagc gtcagcccca tttccgccag ctcatccatg cttagcccgc
     5881 attgccagag atgatttaat gccaccagga ccgtcgcggc attggatgaa ccaccgccga
     5941 gaccgccgcc catcggcaaa cgcttgtcaa tgctgatatt cgcaccgctt cccgtcggaa
     6001 gacgcccgct gtctgccgca gttttcatca acaatcgcgc tgcgcgaacg atcaggttat
     6061 cttcatgttc cacgccttca acgggcgtta acagacgaat atccccatcg tcacgaagct
     6121 caatgctgat ggtgtcgccg taatcaagaa actgaaacag cgtttgcagc gtgtggtaac
     6181 catccgcacg ctgaccggta atgtataaaa acagattaag ttttgccgga gagggccact
     6241 gtgtccgcat tatttcacta tccagttatc catttttaac ttgatgcgtt gaccaccgtc
     6301 ggtgagttcc atattggctg gcatcgcagg atgcgttttg gtgtcataac caccataaac
     6361 aaccttccag tttttgccat tctggctgta ggtaatttcg ctcaggcggt actggtcgtc
     6421 cagtttgtag tcggttgcat cacccggtaa acctaaaatc cactggcgca agctgttgag
     6481 cggaattggc attccggtca atttgccaat catctcttcg gcgtcatcgg cggtataacg
     6541 ctgaccttta ttgtcgacta actgcacgtt acccggttga gcattcagct ccagttccgt
     6601 gctgcccaat gggttagtga gcagcagacg gtagcgatcc tggccggttt gctgccagaa
     6661 aaagcgggcg tacacttttt gttggtcaga aatataagcg aacgcgccgc gagtctgata
     6721 ctgattaaga ttgcgcacgt cttgctgatg ctgacgccat tgtggcgaat ccgggctttt
     6781 gccaggacct ttgggcgtgg taacggaaca ggcagtgagc acaagagcag ccagcggtag
     6841 caggcggata agacgaaaat cgggcagggg catagtgatg acaagtcctt gagatacgtt
     6901 gcagttataa cccttaatgc tagcgttacc gtccgctatc gtctatgttc aagttgtctt
     6961 aattgccaga atctaacggc tttcggcaat tactccaaaa gggggcgctc tcttttattg
     7021 atcttacgca tcctgtatga tgcaagcaga ctaactctat caacgttggt attatttccc
     7081 gcagacatga cccttttagc actcggtatc aaccataaaa cggcacctgt atcgctgcga
     7141 gaacgtgtat cgttttcgcc ggataagctc gatcaggcgc ttgacagcct gcttgcgcag
     7201 ccgatggtgc agggcggcgt ggtgctgtcg acgtgcaacc gcacggaact ttatcttagc
     7261 gttgaagagc aggataacct gcaagaggcg ttaatccgct ggctttgcga ttatcacaat
     7321 cttaatgaag aagatctgcg taaaagcctc tactggcatc aggataacga cgcggttagc
     7381 catttaatgc gtgttgccag cggcctggat tcactggttc ttggggagcc gcagatcctc
     7441 ggtcaggtta aaaaagcgtt tgccgattcg caaaaaggtc atatgaaggc cagcgaactg
     7501 gaacgcatgt tccagaaatc tttctctgta gcgaaacgcg ttcgcactga aacagatatc
     7561 ggtgccagtg ctgtgtctgt cgcttttgcg gcttgtacgc tggcgcggca gatctttgaa
     7621 tcgctctcta cggtcacagt gttgctggta ggcgcgggcg aaaccatcga gctggtagcg
     7681 cgtcatctgc gcgaacataa agtacagaag atgattatcg ccaaccgcac tcgcgaacgt
     7741 gcccaaattc tggcagatga agttggcgcg gaagtgattg ccctgagtga gatcgacgaa
     7801 cgtctgcgcg aagccgatat catcatcagt tccaccgcca gcccgttacc gattatcggg
     7861 aaaggcatgg tggagcgcgc attaaaaagt cgtcgtaacc aaccaatgct gttggtggat
     7921 attgccgttc cgcgcgatgt tgagccggaa gttggcaaac tggcgaatgc ttatctttat
     7981 agtgtggacg atctgcaaag catcatttcg cacaacctgg cgcagcgtaa agccgcagcg
     8041 gttgaggcgg aaactattgt cgctcaggaa accagcgaat ttatggcgtg gctgcgagca
     8101 caaagcgcca gcgaaaccat tcgcgagtat cgcagccagg cagagcacgt tcgcgatgag
     8161 ttaaccgcca aagcgttagc ggcccttgag cagggcggcg acgcgcaagc cattatgcag
     8221 gatctggcat ggaaactgac taaccgcttg atccatgcgc caacgaaatc acttcaacag
     8281 gccgcccgtg acggggataa cgaacgcctg aatattctgc gcgacagcct cgggctggag
     8341 tagcagtaca tcattttctt tttttacagg gtgcatttac gcctatgaag ccttctatcg
     8401 ttgccaaact ggaagccctg catgaacgcc atgaagaagt tcaggcgttg ctgggtgacg
     8461 cgcaaactat cgccgaccag gaacgttttc gcgcattatc acgcgaatat gcgcagttaa
     8521 gtgatgtttc gcgctgtttt accgactggc aacaggttca ggaagatatc gaaaccgcac
     8581 agatgatgct cgatgatcct gaaatgcgtg agatggcgca ggatgaactg cgcaaagcta
     8641 aagaaaaaag cgagcaactg gaacagcaat tacaggttct gttactgcca aaagatcctg
     8701 atgacgaacg taacgccttc ctcgaagtcc gagccggaac cggcggcgac gaagcggcgc
     8761 tgttcgcagg cgatctgttc cgtatgtaca gccgttacgc cgaagcccgc cgctggcggg
     8821 tagaaatcat gagcgccagc gagggtgaac atggtggtta taaagagatc atcgccaaaa
     8881 ttagcggtga tggtgtgtat ggtcgtctga aatttgaatc tggcggtcat cgcgtgcagc
     8941 gtgttcctgc tacggaatcg cagggtcgta ttcatacttc tgcttgtacc gttgcggtaa
     9001 tgccagaact gcctgacgca gaactgccgg acatcaaccc agcagattta cgcattgata
     9061 ctttccgctc gtcaggggcg ggtggtcagc acgttaacac caccgattcg gcaattcgta
     9121 ttactcactt gccgaccggg attgttgttg aatgtcagga tgaacgttca caacataaaa
     9181 acaaagcgaa ggcgctttca gtgctcggtg ctcgcatcca cgctgctgaa atggcaaaac
     9241 gccaacaggc cgaagcgtct acccgtcgta acctgctggg gagtggcgat cgcagcgacc
     9301 gtaaccgtac ttacaatttc ccgcaggggc gcgttaccga tcaccgcatc aacctgacgc
     9361 tctaccgcct ggatgaagtg atggaaggta agctggatat gctgattgaa ccgattatcc
     9421 aggaacatca ggccgaccaa ctggcggcgt tgtccgagca ggaataatgg aatatcaaca
     9481 ctggttacgt gaagcaataa gccaacttca ggcgagcgaa agcccgcggc gtgatgctga
     9541 aatcctgctg gagcatgtta ccggcagagg gcgtactttt attctcgcct ttggtgaaac
     9601 gcagctgact gacgaacaat gtcagcaact tgatgcgcta ctgacacgtc gtcgcgatgg
     9661 tgaacccatt gctcatttaa ccggggggcg agaattctgg tcgttgccgt tatttgtttc
     9721 gccagcgacc ttaattccgc gcccggatac ggagtgtctg gtggagcagg cactggcgcg
     9781 gttgcctgaa caaccttgcc gtattcttga tctcgggacg ggtaccgggg cgattgcgct
     9841 tgcgctggct agcgagcgcc cggactgcga aattatcgct gtagatcgta tgcctgatgc
     9901 tgtctccctg gcacaacgta atgcccagca tctggcgatc aaaaatatcc acattctgca
     9961 aagcgactgg tttagcgcgc tagccgggca gcagtttgcg atgattgtca gcaatccgcc
    10021 gtatattgac gagcaggacc cacatcttca acaaggcgat gtccgctttg agccgctcac
    10081 tgcgctggtt gcggcagaca gtggaatggc agacatcgtg catatcatcg aacagtcgcg
    10141 taacgcgctg gtatccggcg gctttctgct tctggaacat ggctggcagc agggcgaagc
    10201 ggtgcgacaa gcatttatcc tcgcggggta tcatgacgtc gaaacctgcc gtgactatgg
    10261 tgataacgag cgcgtaacgc tcggccgcta ttatcaatga caagtttttc tacactgctt
    10321 agtgttcatc ttattagtat cgcgctttct gttgggctat taaccttacg tttctggcta
    10381 cgttatcaga agcatccaca ggcatttgct cgctggacgc gcattgtgcc gccggttgtc
    10441 gatacggtgt tattgttaag cggcattgcg ttgatggcta aagcgcacat cctgccattt
    10501 tccgggcagg cacagtggct gactgaaaag ctgtttggag ttatcattta tatcgttttg
    10561 ggttttattg cactcgatta tcgtcgtatg cacagtcagc aggcgcgcat tattgccttc
    10621 ccgctggcgt tggtggtgct gtacatcatc attaaactcg ccaccacaaa agtaccgtta
    10681 ctggggtaag tcatgagatc gttagctgat ttcgaattta ataaagcgcc attgtgcgaa
    10741 ggcatgatcc tggcttgcga agcaatccgc cgcgattttc cctcgcaaga tgtttacgac
    10801 gaactggagc gtctcgttag tctggcgaag gaagaaatca gccagcttct gcctttagaa
    10861 gagcagctgg aaaaactgat cgcgctgttt tacggcgact ggggatttaa agcctcacgc
    10921 ggcgtttatc gtctttccga tgcattatgg ctggacccgg tgttaaagaa tcgacagggc
    10981 agtgcggtat cattaggtgc ggttttatta tgggtcgcga atcgtctcga tttgccgctg
    11041 ctgccggtga ttttccctac gcagctgata ttgcgcattg aatgtccgga tggcgaaatt
    11101 tggctgatta atccttttaa cggtgaatcg ttaagcgaac atatgctgga cgtatggtta
    11161 aagggaaata tcagcccgtc ggcggaactg ttttatgaag accttgatga agctgataac
    11221 attgaggtaa tccgcaaatt gctggataca ctcaaagcct cgttgatgga agaaaatcag
    11281 atggagctgg cgttacgcac cagcgaagct ttattacaat tcaaccctga agatccctat
    11341 gaaattcgcg atcgcgggtt gatttatgcg caactggatt gcgaacacgt tgcgttgaac
    11401 gatttaagtt atttcgttga acagtgtccg gaagacccga tcagcgaaat gatccgtgcg
    11461 caaataaata acatcgcgca taaacatatt gtgctgcatt aattaatcga cattttactc
    11521 aagattaagg cgatcctatg aaacaaaaag tggttagcat tggcgacatc aacgtagcaa
    11581 atgacctgcc gttcgtactg tttggcggta tgaacgtgct ggaatctcgc gatctggcga
    11641 tgcgcatttg cgagcactac gtaactgtga cccagaaact gggtatccct tacgtgttca
    11701 aagcctcttt tgacaaagcc aaccgctcct ccatccactc ttatcgtgga ccgggcctgg
    11761 aagaagggat gaaaatcttc caagagttga agcagacttt tggcgtgaaa attatcaccg
    11821 acgttcacga accaagtcag gcacagcccg ttgctgatgt cgtggatgtg attcagttgc
    11881 cggcgtttct tgctcgccag actgacctgg ttgaagccat ggcgaaaacc ggtgcggtaa
    11941 ttaacgtcaa gaaaccacag tttgtcagcc cgggacagat gggtaatatc gttgataaat
    12001 tcaaagaagg cggcaacgaa aaagtgattc tttgcgatcg tggtgctaac ttcggctatg
    12061 acaacctggt tgtcgatatg ctgggcttca gcattatgaa gaaagtgtct ggtaactcgc
    12121 cggtgatttt cgacgtgacc cacgcactgc aatgccgcga tccgtttggc gcagcttccg
    12181 gtggtcgtcg tgctcaggtg gctgagctgg cacgagccgg tatggcggta ggtctggcgg
    12241 ggctgtttat tgaagcgcat ccggatccgg aacatgcgaa atgtgatggt ccatccgcgc
    12301 tgccgctggc taaactggaa ccgttcctca agcagatgaa agcgattgat gatctggtga
    12361 aaggtttcga agaactggat accagcaagt aatctttttt gcttgaaaaa taaagtctta
    12421 gcgttctgcg ttaagacttt tttcatgggt gccagataca aaaaaggccg caggctgtta
    12481 cccctgcggc cggtttcggg cgcatattgc catcacggca gcctgacgcc cgttttcacc
    12541 ttacttccgg ttacgccacc agccgacaat cgctgcggta ataattcccg ccaggatcgg
    12601 tgctgccagg tcgtgccaga aaatcatggc aaactgcgcg agcgtcatat agccgccttg
    12661 ttgtaatgat aacattttgc ggctattctt gaattgttct ggttcaagat tagcccccgt
    12721 tctattgtca ggttaaacct ctcaacgtgc gggggttttc tctttccagc aaccaatgcc
    12781 accagggata aagcccccgc aacattgcgc ctcaccggat aacgccggct tgatgtggat
    12841 actacgtctc aattcatctt cacttcatcc ctgaaatgtt tgcaatgaag agtgcattcc
    12901 ggtttttcaa cagctgttac agtcgtttca tgagtgctct ggatgatgct tccagctcgg
    12961 gttgccaata tttacttgtg gaagagataa agacaaaaat ggccgcaggc tgttacccct
    13021 gcggccggtt tcgggcgcat attgccatca cggcagcctg acgcccgttt tcaccttact
    13081 tccggttatg ccaccagccg acaatcgctg cggtaataat ccccgccagg atcggtgctg
    13141 ccaggtcgtg ccagaaaatc atggcaaact tcgcgagcgt catatagccg ccttgttgta
    13201 atgacaacat tttgcggcta ttcttgaatt gttgatggtt caagattagc ccccgttctg
    13261 ttgtcaggtt ttacctctca acgtgcgggg gttttctctt tccagcaacc aatgccacca
    13321 gggataaagc ccccgcaaca ttgcgcctca ccggataatt ccggcttggt gtggatacta
    13381 cgactcaatt catcttcact tcatccctga aatgtttgca atgaagagtg cattccggtt
    13441 tttcaacagc tgttacagtc gtttcatgag tgctctggat gatgcttcca gctcgggttg
    13501 ccaatattta cttgtggaag agataaagac aaaaatggcc gcaggctgtt acccctgcgg
    13561 ccggtttcgg gcgcatattg ccatcacggc agcctgacgc ccgttttcac ct
//
LOCUS       DAZPEB010000106        13524 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZPEB010000106 DAZPEB010000000
VERSION     DAZPEB010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13524
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.107"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(179..1552)
                     /gene="cysG"
                     /locus_tag="ACHURJ_002650"
     CDS             complement(179..1552)
                     /gene="cysG"
                     /locus_tag="ACHURJ_002650"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFR1281537.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFTTMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            complement(1571..2377)
                     /gene="nirC"
                     /locus_tag="ACHURJ_002651"
     CDS             complement(1571..2377)
                     /gene="nirC"
                     /locus_tag="ACHURJ_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFR1281538.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKSNRPVADKFNQTETAAG"
     gene            complement(2504..2830)
                     /gene="nirD"
                     /locus_tag="ACHURJ_002652"
     CDS             complement(2504..2830)
                     /gene="nirD"
                     /locus_tag="ACHURJ_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFR1281539.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            complement(2827..5370)
                     /gene="nirB"
                     /locus_tag="ACHURJ_002653"
     CDS             complement(2827..5370)
                     /gene="nirB"
                     /locus_tag="ACHURJ_002653"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFR1281540.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRIFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            complement(5632..6813)
                     /gene="tsgA"
                     /locus_tag="ACHURJ_002654"
     CDS             complement(5632..6813)
                     /gene="tsgA"
                     /locus_tag="ACHURJ_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFR1281541.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            7084..7656
                     /gene="ppiA"
                     /locus_tag="ACHURJ_002655"
                     /gene_synonym="rotA"
     CDS             7084..7656
                     /gene="ppiA"
                     /locus_tag="ACHURJ_002655"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFR1281542.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGAPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            7761..7928
                     /locus_tag="ACHURJ_002656"
     CDS             7761..7928
                     /locus_tag="ACHURJ_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFR1281543.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLVELRRHYER"
     gene            7918..8520
                     /locus_tag="ACHURJ_002657"
     CDS             7918..8520
                     /locus_tag="ACHURJ_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFR1281544.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKTKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            8552..9115
                     /gene="pabA"
                     /locus_tag="ACHURJ_002658"
     CDS             8552..9115
                     /gene="pabA"
                     /locus_tag="ACHURJ_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFR1281545.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPGSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            9201..10421
                     /gene="argD"
                     /locus_tag="ACHURJ_002659"
                     /gene_synonym="dapC"
     CDS             9201..10421
                     /gene="argD"
                     /locus_tag="ACHURJ_002659"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFR1281546.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTASTPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(10488..12578)
                     /locus_tag="ACHURJ_002660"
     CDS             complement(10488..12578)
                     /locus_tag="ACHURJ_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFR1281547.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(12629..13261)
                     /gene="crp"
                     /locus_tag="ACHURJ_002661"
     CDS             complement(12629..13261)
                     /gene="crp"
                     /locus_tag="ACHURJ_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFR1281548.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
BASE COUNT         3268 a         3488 c         3550 g         3218 t
ORIGIN      
        1 taagaacgat acaggaatat ttaattggcg tcagtgagcg aatgacttaa ttttgtaagt
       61 cgctgtaacg gtgaatataa cccattagca gataatattt attaaggggt tatgttttat
      121 ttgctgaata tataaaaaga gtcattaaga aggccagaat tctctggccc gggttaaatt
      181 aatggttgga gaaccagttc agtttatcgc gcaggccaac aacccgacca ataataatta
      241 gcgatggact gttcatttgc tgagcaagtt caccgagttg cgtgagcgta ccgtcaatca
      301 cgcgctgcgt gactgccgtt ccgttttcga caattgccac cggcatttcg ccaggcatac
      361 cgtgttcaat cagcttttgc tgaatagtcg cggcctgatt caaccccata tagaacacca
      421 gcgtctgttt ttctgccgcc aggttttccc agtccagctc gccaccggtt tttaagtgtc
      481 cggtaattaa gcgtacgctc tgggcataat cgcgatgcgt gagtggaata cccgaatagg
      541 cagagcaacc agaagctgcg gtaatacccg gaaccaccga gaacggaata cccgcgttgc
      601 acagtgtttc cagctcttcg ccaccacggc caaaaataaa cggatcgcca cctttcagcc
      661 gcaccacgcg tttgcctttt tgcgcttccc gcagcaggat ctggttaatc tcttcctgcg
      721 gtacacagtg gtatcccgcg cgtttgccga cgaaaacgcg atcagcatcg cggcgtacca
      781 gattcataat atcgtcagaa accagacggt cgtagaccac cacatctgcc tgctgaattt
      841 gttgcagtcc tttcagcgtc agcagcccgg catcgcccgg acctgcacca accagcacca
      901 cttcaccgcg atggtcgagc ggttcgttga ttaactgttc ggtcgtttca gtaatggctt
      961 tctgatcgtt gtttgccagc gactgcgcca ggcggtcgtt aacgaacaat ttctcccaga
     1021 aacggcgacg ctcactcatc gtggtgaact gttgtttcac tcgcccgcgt aattgcccgg
     1081 catatttcgc cacctggccc agatgcaacg gcagcagtga ttcgagtttt tcgcgcaaca
     1141 ggcgcgccag aaccggagag gtgccgccag aggagaccgc taccattagc ggtgagcggt
     1201 caataatcga cggcataata aagctggcgg ctttcggcgc atcgactacg ttacagaaga
     1261 tgcgacgagc ttcagcggct tcgctgacgc gctggttaag cgtgtcatca tccgtcgctg
     1321 caatcgccag ccagcaggtg tcgagaaggc tttcatcaaa tggcccttcg acgagggtta
     1381 acatgcctgc atctgcccat gcggtgaact gtggaataaa cgctaatgca ttcaccgtta
     1441 agcgagcgcc tgcgtctaac agcaaccttg ctttgcgttc cgcgacatca ccaccgccga
     1501 caatcagaca gtcgcgatcg cgtaattggc aaaatatagg caaatgatcc acgtaaaaac
     1561 cccttagtaa ttaaccggca gccgtttcag tttgattaaa tttgtccgca accggacgat
     1621 tcgatttcgg cgtagcatac caataaccca atcccatgaa tacggcacct gataaagtat
     1681 tacccagcgt cacccacagc aggttatgac caatacccgc cagcgtgtag gcttcgctgt
     1741 ggttgccgaa ccaggagagc gcgaacagcg tcatgttagc gatagagtgc tcgtagccgg
     1801 acgcgataaa tgccagcaga caccaccaga tagcgataaa tttcgccgcc ccttcagtgc
     1861 gcagcgccat ccagattgcc aggcaaacca gccagttaca caatgcacct ttgaagaaga
     1921 gtaccattgc cggtgcagtg gttttagcca gcgcgacgga gtgaacgatg ctggtatcta
     1981 ccggcagcag gctaccgccg ccccagctat agagcatggc aacgaagacg gaaccgacca
     2041 ggttacccag ccaggtttgc ggcaggattg cccacatttg cccgtggctg atgctgcccg
     2101 cttttacccc aaaggtgagg aacatggtgt gtccggtgaa cagttcagaa ccggcgataa
     2161 tcaccagcgt taaggcgata ccaaaggtcg cgcccatcac cagcggacgt acggacgggt
     2221 cgagcaaatt accgagcgtg aaaatcaaga tgatcccaag acccacatac gcgcccgcca
     2281 tggcggagct gacccaaaag ccgagcgggt tatttgccga caggcgtgca atgcgcgcag
     2341 cgttagccgc acacttatta atagtgtctg taaacatttg attatccttt aaaaaataaa
     2401 aacaaaaaaa taaaacaaaa agtttaaaaa atgtcctgca atataccgaa ggtaattaca
     2461 ggaccatgca aaaaggggag gcattgcgcc tcccgttaaa acattaaccg cgcagctgca
     2521 ccacgccgtc tttcactcgt gcttcgtaat gtttgacgga aaactgttcg tcttccatgc
     2581 acaagccatc acttaagcga aaacgctgtt ttttcagcgg gctggcgacc cacagctcgc
     2641 cctggtgttc cgcaatcagc ccgcgtgaca gcacgctgga ctcgaagaac ggatcgatgt
     2701 tgctgatcgc aaacacctgg tcgctgtgat acgggcggaa aatcgcgacc tgctcgtcgc
     2761 ctaacagcgc gcagacgccg gtttcaggca ggatgtcatc gattttgcag atgtctttcc
     2821 actggctcat gcgttgtcct ccaccagagt taccgggata cgttcatacg gcgttgccgg
     2881 acggtgctgt tcgcgctctg gcaccatctg cacgttcggg tcacgcttgt cgctgttgat
     2941 gaagtgtttg aagcgagtct gcgcggacgg tgtattgacc gtttcagtcc actcacacac
     3001 taccgcttca cgcaggcgcg ccatctcttc ttccagatga gcgttcagcc ccagtttgtc
     3061 gtcaatgatc accgctttca ggtaatcgat gccgccttcg aggttttcta accacggcgc
     3121 ggtacgcgtc agtttgtcgg cggtacggat gtagaacatc atgaagcggt cgagatattt
     3181 gatcagcgtt tcgcgatcga tatcagctgc cagcagatcc gcatgacgcg gtttcatgcc
     3241 gccgttgccg caaacataca ggttccagcc tttttcagtg gcgataatac ccacgtcttt
     3301 accctgagct tctgaacatt cacgggtaca gccggagaca ccgaacttca ttttgtgcgg
     3361 cgtacggatg cctttgtagc ggttttccag ttccacgccg aggccgacgc tgtcgccaac
     3421 gccgtagcgg caccaggtgc tacccacgca ggttttcgcc atacgcagtg ctttcgcata
     3481 ggcatgaccg gtttcgaagc cggcttcaat cagctgacgc cagatctctg gcagatcgtc
     3541 tttctgtgcg ccaaacatcg ccaggcgctg ggaaccggtg attttggtgt agagattaaa
     3601 ttcacgcgcg atacgaccca ctgccatcag cccttccggg gtgatttcac cgcccggaga
     3661 acgcgggatc accgagtagg tcccgtcttt ctggatgtta gcgaggaagt tgtcgttaga
     3721 atcttgcagc ggagtatgtt ctggcttcag aatgtattcg ttccagcagg aggccagcag
     3781 cgaaccgacg gtcggtttac aaacttcaca accgtagcct ttgccgtgtt tcgccagcag
     3841 ttcttcgaag gttttaatgc cttcaacgcg gatcaaatgg aacagttcct gacgcgaata
     3901 agcaaagtgt tcgcacaggt tgttgttaac ttcgatgccc tgtttcgcca gttccgcgtt
     3961 cagtacctga gtgaccagcg ggatacagcc accgcagcca gtacccgctt tggtttcagc
     4021 tttcagcgcc gcaactgtgt ggcagccttt gttgatggca gcaatcagat cacctttagt
     4081 gacgtcgaag caggagcaga tttgcgcgct gtccggcagt ttatcaacac cgatagacgg
     4141 cttaccgcta cccgagtgcg ccggcagaat cagagaatcc gggttttccg gcagctcgat
     4201 agcattcagc accagttgca gcaagttacc gtagtcgctg gtatcgccca ccagtaccgc
     4261 accgagcagg gttttgttgt cttcgctgac gatcaggcgt ttgtagatct ctttgctttc
     4321 gtcgaggtaa acgtagctac gtgcgccagg cgtgcgacca tgagcatcac caataccgcc
     4381 tacgtccacg cccagcagtt tcagcttggc gctaaggtca gcaccttcaa aggcgttttc
     4441 gctaccgaga atatggtcaa cggcgacctg cgccattttg tagccaggtg ctaccagacc
     4501 aaatatacgg ttgttccagc ttgcgcattc accgatagcg tagatatccg gatcggaagt
     4561 ttggcaggaa tcattaatga cgataccccc acgcggagca acgtccagac cacactgggt
     4621 tgccagctta tcgcgcggac ggataccggt agagaagacg ataaagtcga cttccagttc
     4681 gctgccgtcg gcaaaacgca tggttttacg tgcttcgaca ccttcctgca caatctcaag
     4741 ggtgtttttg ctggtgtgaa cgcgcacgcc catactttcg attttgcgac gcagctgctc
     4801 gccacccatc tgatcaagct gttccgccat cagcataggg gcaaattcga taacgtgggt
     4861 ttcaatacct aagtttttca gcgcgccagc ggcttccaga cctaacaggc cgccaccaac
     4921 aacggcaccg cgtttgctgc gacgggcgca ggattcaatg gcgttgaggt cttcaatggt
     4981 gcgatagaca aagcagtcct gagtatcaga acctttgatt ggtgggatcc acgggtagga
     5041 accggttgcc atgatcagct tgtcataaaa aacggtacgt ccggcgctgg agtgaatcac
     5101 cttctcctga cggttgatgg tgatagcgcg ttcgccgacc agaactttga tgccgtgttt
     5161 ctcgtagaag ccttcgcgca ccagcgacag ctcttcggcg gtgtggtgag agaagtaaga
     5221 cgagaggtgt acgcggtcat aagcgatgcg cggttcttca cagaaaacgg taatatcaaa
     5281 gttggccgca tcagatttat caagaagatc ttcgataaag cgatggccga ccataccgtt
     5341 accgataatt gcgagtctga ctttgctcat ttttgcctcg atttcttttc tattaccgcc
     5401 taccttaacg attcagcaac cccgcttatt gatgtaaatc aaattcacct ttatataccc
     5461 cttaaggggt atattgctga tttgtataaa ttttcttaag tcacggaatt gtctgacatt
     5521 tcatgtttgt gcaaaaaata gacaaaatga acgggtttat agaaatagat gcacgtagcg
     5581 gggttagaga ggaaaaggcc ggacgacgat agtgccgtcc ggcattagca attaatgaga
     5641 ggtcagggtg ttatgctgac ggtgacggct gacgaaacct aacaggaagc acatcacaaa
     5701 gacgacagcg tacagaccgt ttgctgtaag cagtgccgcc tgcggaccgc tatgttcaac
     5761 aatcgggccg gtaaccacaa aggtcaacat agtaccgatg gttccgcagg tcaggacaaa
     5821 gttaaccagt tttggcgacg gtactttggt ctgctgtgaa cccagggtga tgatggtggt
     5881 atagatcgcg ctggagaaga agcccagagc gagaattgac cacgccatat gtgctggtgt
     5941 tccggtgtta aagacgtaca tcagaatcgc agccagacca gccagtacgg tcagaatgcg
     6001 ttgcaaatca aagaagcgaa gaataaagct gaacgcccac atgccgacca tgtatgacat
     6061 ccagaagtta ctcaccagcg tgcccgcgtc gttcaggctc atgcccaggc ctttggcata
     6121 ctcaggcacc caggagataa aacctaactg accgaggatg tagcacagcg ccgcaacaga
     6181 gagaaacagt acgccgatcc cccacttttc tttctctacc ggagcatccg tttttggtgc
     6241 atgtttgccc agtgctggga actcacagcc gaaggtcaga ataaaaatag cgacatacac
     6301 cagcccgatg caggcataaa cccagtacca ctcaatgctg cgcgccagta gaaacgcggc
     6361 gatcattggg aaaatcatcc cagccatact gaagaaggag tcagtaaata acaggcggga
     6421 accgcgctga cgcccttcat acatttgtgt tatcaggaat gtaccaatcg acatagtaat
     6481 gccgctgacc accccgagaa tgaacatcgc cgtcgagaac agcgccaggc tgtggctgaa
     6541 catcaaaccg gcaaccgcca gcaccatcag gagaaagcca aaacgtaact gcgttttcaa
     6601 cgggacgatt tccatcagcc aggcgttgag gaagatagag attaaaatgc cggcgttgag
     6661 gaaggtgaag gtgttactca tactggaaac aggcagattg aaataatcgg cgatatttcc
     6721 catcaccatc ccggtgacaa taaccaacgc accagtcagt gcgtaggaga gaaagctaat
     6781 ccatgtgagc ttgatgcgat tgctgttagt catgattggc ctgcgttcaa aaataaaatg
     6841 gcatagcggg atatgccgcg agcgggtgat tttaggtgat tttgtgatct gtttaaatgt
     6901 tttattgcaa tcggttgcta aattgcattt taagaagtga tcttgatcac ggaataaaaa
     6961 gtgatcgtca ggttacatat atttcagata cgtaaaatta ggtaaaggga tggccttgtt
     7021 cttgaaggct atttagaatc tcttcacttg ctttttttct gctctgtttg ttaaggaaat
     7081 ctcatgttca aatcgaccct ggcggcgatg gctgctgttt tcgctctttc tgctctttct
     7141 cccgcagcaa tggcagcgaa aggggccccg cacgtattgt tgacaacctc agctggtaac
     7201 atcgaactgg agctggataa acaaaaagcg ccagtgtctg tgcaaaactt tgtcgattat
     7261 gtgaacagcg gtttttataa caacactacc tttcaccgcg tcattcctgg ctttatgatt
     7321 cagggcggcg gtttcaccga gcagatgcag cagaaaaaac caaacccgcc aatcaaaaat
     7381 gaagccgata acggcctgcg caacacgcgt ggcaccatcg cgatggcgcg taccgctgac
     7441 aaagatagcg ccaccagcca gttctttatc aacgttgccg ataacgcctt ccttgaccat
     7501 ggtcagcgtg atttcggtta cgcggtattt ggtaaagtgg tgaaaggcat ggacgtggcc
     7561 gataagattt cccaggtgcc gactcatgac gttggtccgt accagaatgt gccgtcaaaa
     7621 ccggtagtta tcctttccgc taaagtcctg ccgtaatgat ttctcgcgcg ggcaatcttg
     7681 cccgcgcttc tgctctcccg gcgtaacccg gatttgccgc ttatacttgt ggcaaatgga
     7741 cacgttcagg gaggcatcaa gtgaagaaac tcaccgataa gcaaaagtcc cgtctctggg
     7801 agcttcagcg taatcgtaat ttccaggcca gtcgccgtct tgaaggcgtc gagatgcctt
     7861 tagtcactct tactgccgca gaggctttag cgcgccttgt agagctgagg aggcactatg
     7921 agcgataaat tcggcgaagg gcgcgatccg tatctttatc caggccttga tatcatgcgt
     7981 aaccggctga acatccgcca gcaacaacgg ctggaacagg ccgcttacga aatgacggcg
     8041 ctgcgtgctg cgaccattga gcttggtccg ctggtgcgcg gtttaccgca tttgcgaact
     8101 atccatcgcc agctgtatca ggatattttc gactgggcag ggcaactgcg tgaagttgat
     8161 atttatcagg gcgatacgcc gttctgccac tttgcttata tagaaaaaga gggcaatgcc
     8221 ctgatgcagg atctggagga agaaggttat ctggttggcc tggagaaaac gaagttcgtc
     8281 gagcggctgg cgcattacta ttgtgaaatc aacgtgctgc atcccttccg ggtgggaagt
     8341 ggtctggcac agcggatctt cttcgagcaa ctggcgattc atgccggata tcaactgagc
     8401 tggcagggta tcgaaaaaga ggcctggaat caggcaaatc agagtggggc aatgggggat
     8461 ctcaccgcac tgcagatgat atttagcaaa gtggtaagcg aagccgggga atctgagtaa
     8521 aatagcgcgg ttctttcgta ccggagccgc catgatcctg cttattgata actacgattc
     8581 ttttacctgg aacctctacc agtacttttg tgaactgggg gcggatgtgc tggttaagcg
     8641 caacgatgcg ttgacgttgg cggatatcga cgcccttaaa ccacaaaaaa ttgtcatctc
     8701 acccggcccc tgtacgccag atgaagccgg gatctccctt gacgttattc gccactatgc
     8761 cgggcgcttg ccgattcttg gcgtctgcct cggtcatcag gcaatggcgc aggcatttgg
     8821 cggtaaagtt gtgcgcgccg caaaggtcat gcacggcaaa acctcgccga ttacacataa
     8881 cggtgagggc gtatttcggg ggctggcaaa tccacttacc gtgacacgct accattcgct
     8941 ggtggtggaa cctggctcat taccagcgtg ctttgacgtg acggcctgga gcgaaacccg
     9001 cgagattatg gggattcgcc atcgccagtg ggatctggaa ggtgtgcagt tccatccaga
     9061 aagtattctt agcgaacaag gacatcaact gctggctaat ttcctgcatc tctgatttcc
     9121 gattgccatt cagtgatttt ttatgcatat tttgtgatta taatttcata tttatttatg
     9181 cgtaacaggg tgatcatgag atggcaattg aacaaacagc aattacacgc gcgactttcg
     9241 atgaagtgat cctgccgatt tatgctccgg cagagtttat tccggtaaaa ggtcagggca
     9301 gccgaatctg ggatcagcaa ggcaaggagt atgtcgattt cgcgggtggc attgcagtta
     9361 cggcgttggg ccattgccat cctgcgctgg tgaacgcgtt aaaaacccag ggcgaaactc
     9421 tgtggcatat cagtaacgtt ttcaccaatg aaccggcgct gcgtcttggg cgcaaactga
     9481 ttgaggcgac gtttgccgaa cgcgtggtgt ttatgaactc cggcacggaa gctaacgaaa
     9541 ccgcctttaa actggcacgc cattacgcct gtgtgcgtca tagcccgttc aaaaccaaaa
     9601 ttattgcctt ccataacgct tttcatggtc gctcgctgtt taccgtttcg gtgggtgggc
     9661 agccaaaata ttccgacggc tttgggccga aaccggcaga catcatccac gttcccttta
     9721 acgatctcca tgcagtgaaa gcggtgatgg atgatcacac ctgtgcggtg gtggttgagc
     9781 cgatccaggg cgagggcggt gtgacggcat cgacgccaga gtttttgcag ggcttgcgcg
     9841 agctgtgcga tcaacatcag gcattattgg tgtttgatga agtgcagtgc gggatggggc
     9901 ggactggcga tttgtttgct tacatgtact acggtgtgac gccggatatt ctgacttctg
     9961 cgaaagcgtt aggcggcggc ttcccgatta gcgccatgct gaccacggcg gaaattgctt
    10021 ctgcgtttca tcctggttct cacggttcca cctacggcgg taatcctctg gcctgtgcag
    10081 tagcgggcgc ggcgtttgat atcatcaata cccctgaagt gctggaaggc attcaggcga
    10141 aacgccagcg ttttgttgac catttgcaga agatcgatca gcagtacgat gtgtttagcg
    10201 atattcgcgg tatggggctg ttaattggcg cggagctgaa accacactac aaaggtcggg
    10261 cgcgtgattt cctgtatgcg ggtgcagagg ttggcgtaat ggtgctgaat gccggaccgg
    10321 atgtgatgcg tttagcaccg tcgctggtgg tggaagatgc ggatatcgat gaagggatgc
    10381 aacgtttcgc ccacgcggtg gcgaaggtgg ttggggcgta aataagacgc atcaggcaaa
    10441 atatgtcgga tgcggcgtga atgccttatc cgatggttta gcctgtatta cgccttcgaa
    10501 tcccgcaact tgcgactcag ccaaatcccg tgatgcggtc gctgacgcca tgccatcgac
    10561 gaaatggtgt gcatggtgtt cagatgacca ataacccgct gtaaatgctg ctccagcgta
    10621 cctgccgcgc cttcgtgcgg ctgcatctcc ggcgcatcca tgatattggc atcgccagaa
    10681 ctacccggtt cgtcatactc cagtcgctgc tgacaacgct gaatggcgat ttcacaagac
    10741 tgtaaatact cttgtgccag ttcaggtggc aatgcccggt gttcccgcgc cagcgtggtc
    10801 atggcattaa tatgctcaac aataaactgg ctgtgcgtta cccacagttt catatctgcc
    10861 agataatggc tgttaaacgc cggttcctgc atcgcctgat tcaatgagtt atacagagtg
    10921 ttatgtgcct gatttacccg cattcgctgc caggccagtg gcgtaggttg cggatcctcg
    10981 ctaagaatca agcgaatcgc ttcctgatag gcttctaaag cgtcatgggc gtttttacgc
    11041 aataacccgc tctgccactg cggccacagc cagacagtac cgccgaaagc aattaaacaa
    11101 ccaataatgg tatcgataag acgcggaagg atgtattgct cgccgttcaa ccacaatagt
    11161 tgcagggtat acactgcggt aatagtaaaa ccgaccgtcg cccagccgta gtttttgcgc
    11221 aatatcaggt agctggcgag ggtggtaatc agcatcaacg tcagggtgta accttcggga
    11281 attttaaagt gcagcgccac gcccgcaatg attaacccga ccacggttcc cacggagcga
    11341 ttcacaatcc tcagacgggt tgcgccatag ccattttgtg tcaccagcaa taccgtcatc
    11401 aggatccagt acgacttcgg cagatgcagc gcggtgccca tcaggctggc aacgcttaac
    11461 atcacactga gtcgtccggc attgcgtagc gccggagact ttagtgacag ataacttttc
    11521 agcgcaggaa gtaatggcat tcgccgctgt ttatcggcca gtaagtcacg ggcatagagc
    11581 ggtttttggg tgcgcagcac gcgggcgatg cggctgaaat gccagtagca gaattgccca
    11641 accggattat ccggatgctg gcgggcgatt ttttccagtg cgccaatttg cttttccatc
    11701 gtaaaacgcg ttggcaggcg atggtaaaga atgtcatcag ccagcacgcg caggcgagcg
    11761 gcgacggttt gcgcattcca gcggataact tcttccgcat ggctacgctc gaccagcttt
    11821 tgcacctctt ccggctgatg caaactgacc gaaatatgtt cctgtaaatc cagcgcctcc
    11881 tggaaaatac gcagcatccg cttgtagtca gtattatttt gcgcggaaag catatgcatt
    11941 tgctgatagc actgggtaat tagatcgacc gctttttgct ggcgcaccag cagcggcggc
    12001 agcgcttttt cagggtcggt gtgctgggta agcaggctgt atttggcttc acaataatct
    12061 gccagttcac ggtacagcag acttagtgac tcgcgcagcg gttgttcgcg ccagatccag
    12121 aaccaaaacc agttaaacaa tccgtaccag agagtgccca acgcatagat gagcaacggt
    12181 tcccagaccg gcatgtatcc cgccagactg agggtaaaaa tggctgccag cagcgatgcg
    12241 ggaagcaatt ttgcgtgcaa tggccccaac tcagcagtga cgccaagtac cagcgttaat
    12301 ccggtcagca aaaagggcag gggaacatct tttgccagta gcagctgtgt cagtaagcta
    12361 caggtggcaa acagcgacgc accaatgatt aagcgtttga aaaaacgttt atgaggcgta
    12421 tcaaggcccg caatattgca acaggcagga acgagagaga agagcagacc gaatcgtaat
    12481 tcgccaagca ttaacccaac ggccacgggc aaacatagca ccagcgtttg tcgaagtgca
    12541 tagttgatat cggggtgata aatcagtctg cgccacatcg ggggaaacaa aatggcgcgc
    12601 taccaggtaa cgcgccactc cgacgggatt aacgagtgcc gtaaacgacg atggttttac
    12661 cgtgtgcgga gatcaggttc tgatcttcaa gcatcttcag aatgcgtccc acggtttcac
    12721 gagaacagcc gacaatctga ccgatttcct gacgggtaat tttgatttgc ataccgtccg
    12781 ggtgagtcat agcgtctggt tgttttgcca gattcagcag agtctgtgca atgcggcccg
    12841 tcacgtcgag gaacgccagg ttgcccactt tctctgaagt gacttgcaga cgacgcgcca
    12901 tctgtgcaga cagacgcatc agaatgtccg ggtttacctg aatcaattgg cgaaattttt
    12961 tgtacgaaat ttcagccact tcacaggcgg ttttcgcacg tacccatgcg ctacgttcct
    13021 ggccctcttc aaacaggccc agttcgccaa taaaatcacc ctgattcaga taggagagga
    13081 tcatttcttt accctcttcg tctttgatca gcactgccac agagccttta acgatgtagt
    13141 acagcgtttc cgctttttca ccctggtgaa taagcgtgct cttggatggg tacttatgaa
    13201 tgtggcaatg agacaagaac cattcgagag tcgggtctgt ttgcggtttg ccaagcacca
    13261 tgcgcggtta tcctctgtta taagctttct ccagagccag ataacgccgc tgtctctgga
    13321 ttgccgaaat atgcttcccg ctacctggga aggggctatc aactgtactg cacggtaatg
    13381 tgacgtcctt tgcatacatg cagtacatca atgtattact gtagcatcct gactgtttta
    13441 gcatagcttt cgctttgtgt ctcctggtgt ctcgcttcag catgacccag gtcgccttcc
    13501 gttgcgcgat ttggttagta cgcg
//
LOCUS       DAZPEB010000107        13522 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZPEB010000107 DAZPEB010000000
VERSION     DAZPEB010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13522
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.108"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..186
                     /locus_tag="ACHURJ_002662"
     CDS             <1..186
                     /locus_tag="ACHURJ_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFR1281549.1"
                     /translation="CWSLAAGANPSFIASQMGHTNAQMVFNVYGAWMKDNNHEQIELL
                     NRRLSESVPCMPHKKAG"
     gene            207..908
                     /gene="mlrA"
                     /locus_tag="ACHURJ_002663"
     CDS             207..908
                     /gene="mlrA"
                     /locus_tag="ACHURJ_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFR1281550.1"
                     /translation="MGLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYP
                     AQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARRKQGKDALVVGW
                     NIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQL
                     TSWQEQGHDIFPLGI"
     gene            968..1075
                     /locus_tag="ACHURJ_002664"
     CDS             968..1075
                     /locus_tag="ACHURJ_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFR1281551.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(1056..1787)
                     /gene="yehW"
                     /locus_tag="ACHURJ_002665"
     CDS             complement(1056..1787)
                     /gene="yehW"
                     /locus_tag="ACHURJ_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFR1281552.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFVVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(1792..2718)
                     /gene="yehX"
                     /locus_tag="ACHURJ_002666"
     CDS             complement(1792..2718)
                     /gene="yehX"
                     /locus_tag="ACHURJ_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFR1281553.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVCREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLEEA"
     gene            complement(2711..3868)
                     /gene="yehY"
                     /locus_tag="ACHURJ_002667"
     CDS             complement(2711..3868)
                     /gene="yehY"
                     /locus_tag="ACHURJ_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFR1281554.1"
                     /translation="MTYFRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPGKKGSICALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(3875..4792)
                     /gene="osmF"
                     /locus_tag="ACHURJ_002668"
     CDS             complement(3875..4792)
                     /gene="osmF"
                     /locus_tag="ACHURJ_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFR1281555.1"
                     /translation="MPLSKVWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDSEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQISGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(5044..7341)
                     /gene="bglX"
                     /locus_tag="ACHURJ_002669"
     CDS             complement(5044..7341)
                     /gene="bglX"
                     /locus_tag="ACHURJ_002669"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFR1281556.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPFTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDMVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            7537..9252
                     /gene="dld"
                     /locus_tag="ACHURJ_002670"
     CDS             7537..9252
                     /gene="dld"
                     /locus_tag="ACHURJ_002670"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFR1281557.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPSSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALQRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(9291..10223)
                     /gene="pbpG"
                     /locus_tag="ACHURJ_002671"
     CDS             complement(9291..10223)
                     /gene="pbpG"
                     /locus_tag="ACHURJ_002671"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFR1281558.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAAITASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(10397..10984)
                     /locus_tag="ACHURJ_002672"
     CDS             complement(10397..10984)
                     /locus_tag="ACHURJ_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFR1281559.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            11154..11732
                     /locus_tag="ACHURJ_002673"
     CDS             11154..11732
                     /locus_tag="ACHURJ_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR1281560.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(11862..12623)
                     /locus_tag="ACHURJ_002674"
     CDS             complement(11862..12623)
                     /locus_tag="ACHURJ_002674"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR1281561.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(12676..>13522)
                     /locus_tag="ACHURJ_002675"
     CDS             complement(12676..>13522)
                     /locus_tag="ACHURJ_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HFR1281562.1"
                     /translation="QAALNTVLQQIEKEQNTIIATDRQLYQNGITSSVEGVETDINAS
                     KTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKPVALPKVASQLPDELGYSLLARRA
                     DLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQDALHLSDLFRHSAQQMGVTAGLT
                     LPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAVNDVARAASQVQTLAEKNQHQAQI
                     ERDTLRVVGLAQARFNAGIIAGSRVSEARIPALRERANGLLLQGQWLDASIQLTGALG
                     GGYKR"
BASE COUNT         3238 a         3779 c         3417 g         3088 t
ORIGIN      
        1 tgctggtcac ttgccgcagg agctaatcct agttttatcg caagccagat gggccacaca
       61 aacgcacaaa tggtattcaa tgtttacgga gcatggatga aagacaacaa tcacgaacag
      121 atagaacttc ttaacagaag actatctgaa agtgtcccat gcatgcccca taagaaagcg
      181 gggtaaaata aaaacttgca aaatcaattg gtttaccttt aatccctgtc acgttacgcg
      241 cgtggcagag gcgttacggg ttgctgaaac cgcaacggac agacggcggt catcgactgt
      301 tcaacgatgc cgatattgac cggatccgcg agatcaaacg ctggatcgac aacggcgtgc
      361 aggtcagcaa agttaaaatg ctgctcagta atgaaaatgt tgatgtgcag aacggctggc
      421 gcgatcagca agaaacatta ctgacctacc tgcaaagcgg caatctgcat agcctgcgaa
      481 cgtggatcaa agagcgcggt caggattacc ccgcccagac actcaccaca catctgttta
      541 ttcctctgcg ccgacggctt cagtgccaac aaccgactct ccaggcgctg ctggcgatcc
      601 tcgacggcgt actgatcaac tacatcgcca tttgtctggc ttcggcacgt agaaaacagg
      661 gtaaagatgc gctggtggtt ggctggaata ttcaggatac cacccgtctg tggctggagg
      721 gctggattgc cagtcaacaa ggatggcgca ttgatgtcct cgcccactcg ctcaatcaac
      781 tacgccctga actattcgaa ggccgtacat tgctggtgtg gtgcggtgaa aatcgaacct
      841 ccgcccaaca gcagcaactc accagttggc aagaacaagg ccatgatatt ttcccactcg
      901 gcatttaatg attcgttaac aaatgcgctt tactgtacaa tcctttcgtt aacataagga
      961 gtgcaatatg cgcatagcta aaattggggt catcgccctg ttcctgttta tggcgttagg
     1021 cggaattggt ggcgtcatgc tcgcaggtta tacctttatt ttgcgtgctg gctaagcgcc
     1081 tgcaccagcc tttcaaacag gcggtctgca atgatcgccg ccagtgccac cagtaacgcc
     1141 ccctggatca catacgcggt attaaatccg ctaagcccga tgatgatggg cgtacccagc
     1201 gtgctggccc ctaccgttga ggcgatcgtc gccgtaccaa tgttgataat caccgaagtt
     1261 cgcacgcccg ccagaatcac cggagccgcc agcggtagct cgaccttacg cagtcgctga
     1321 ccacgactca ttcccatacc tttcgcaact tctgtcacgc tggcatcaat cgctcccagc
     1381 ccggcaagtg tcgcctgcag gacgggcagc acaccgtaaa ggatcaaggc gataatcgct
     1441 ggttgcagac caaagccgat caccggaacg gcaatcgcca gcactgcgac aggcggaaaa
     1501 gtctgtccaa cggcggcaat agtttccacc agtgggcgaa attccgcgcc ccacgggcga
     1561 gtgacagcaa ttccggcacc agtgccaatg atcaccacaa acaaactcga aattcccacc
     1621 agccagaaat gagccagtgc cagagctgca aaactttctt gctgataaac gggtcgtggc
     1681 agttgtggga acaaggcagc aaacagcggc tggctgtaag gcagccagaa aatcagtgcc
     1741 acaaacaaag caatgagcca gaacagcgga tcgcgcaaca tcttcatacg cttacgcctc
     1801 ctccagcaga tcctgaaaat gcagcgtgcc gcaaggctgg ccctgcgtgt tcaccaccgg
     1861 cagcacctcg catccccgcg cgacaaacag ggagagcgca tcgcgtagcg tcatctcttc
     1921 tgccagtgcc tcaccttctg cccgttcttc gcgacacacg taatccgcca cactacgtaa
     1981 cgaaagcagg cgcacaccca gttcgctgcg cccaaaaaac tggcggacaa aatcgtttgc
     2041 cggacgagtc agcatcgtca gcggattgcc ctgctgcacc acttcaccgt gatccatcaa
     2101 taccagatgt tctgccagcc gtagcgcctc atcaatatca tgagtgacca gcacaatggt
     2161 acgccccagc aaacggtgaa tgcgcgtcat ctcttgttgc aacgcgccgc gcgttaccgg
     2221 gtccagtgcg ccaaaaggtt catccatcag taagacttgc ggatcggcag ccagtgcgcg
     2281 cgccactccc acacgttgct gctgaccacc ggaaagctga tgcggataac gctcacgtaa
     2341 atttgactcc agccccagta gcgccattaa ttcgtcgata cgatcgtcaa tccgcgcccg
     2401 cgaccatttt tgtaattgcg gcacggtagc gatgttttgc gccacgctcc agtgggggaa
     2461 caggccaata gattgaatgg catagcccat ccggcggcgc aactccagta ctggcagcga
     2521 gcgaatttct tctccggcaa agcggatcac tccgctgtca tgctccacca ggcggttaat
     2581 cattttcagg gtggtggatt tgccggagcc agatgtgcca atcagcaccg aaaaactccc
     2641 ttcctgaaaa ttgagattga gatcgttaac ggctttttgt gcgccgaaca gtttgctgac
     2701 atggctaaat tcaatcatta cgtttcacct tcagcagtgc gataagcaaa tcgaacagcg
     2761 cgtcggtaag caccgccaga acaattaccg ggatcacccc cagcaacact aaatcaatgg
     2821 cgctgcttag cagcccctgg aaaaccagcg caccaaaacc gcctgcgccg attaacgccg
     2881 caatcaccgc catacctaca gtttgcacca tcaccacccg caggctgcgc agaaataccg
     2941 gtaacgccag tggtaactga acatgcagga atcgctgcgc cccgctcatg cccatcgctc
     3001 tggcactctc cagcacatcg cgcgggatct ggttcaaacc gactaccacg ccgcgcacca
     3061 gcggcagcaa ggcatagagc accagcgcaa tcagtgcggg tgtcattccg gttcctgcta
     3121 tgccgagctt ccccagccac ggaaaggccg tcaccagcgc ggcaagcggc gcaatcaaca
     3181 ggccaaagag cgccaccgaa ggcacggtct gaatgacatt gagcagagaa aaaattgccc
     3241 cctgccgagc agtggaaaag tagcaccaga tgcccaacgg cacaccaatc actaacgcag
     3301 gcagcaccgc accaaacagc aacgtcagat gttgtgccag cgcgtcgtca aacacatcct
     3361 gacggttggc gtattctttc attagtgaga gatcgttaag cgtgccggag tacagcaacc
     3421 acagcggaat aatggcaatc tgcatatgca acaaccagcg ccacagcgga tgcgtggaga
     3481 ttcggcggat ggcatcgcta caggccagca atgccagcgc cgcagccagc cagaaaccac
     3541 tgccgaggct ggtacgcgcc agcgcactgc cattttgcgc cagttgtgtc gccgcctttc
     3601 ccgcgcccca caccagcaat acgaagacga attgcgccag aatgagtgca caaatgctgc
     3661 cttttttacc gggaataaaa caggccgtca gccaggcgca accaacgccc accagcatcc
     3721 agatcgtttg cggccacagc tgccagagat gacgcccctc acccgaaact aaacgattag
     3781 gcgcgtaact gataaacggc agcgccgctg cgattgccgt cagcaacagc agcagcgcca
     3841 gaacaggatt aatacggaaa taagtcacgg gaaattactt cgtccaccct ttttgtttca
     3901 ggtagtcggc agccactttt ttggcatcca gtccttccac agcaatgctg gcattcagtt
     3961 gctgcaatgt ttttgcatcg aggctggcga agactggctg tagccactgt gccatttgcg
     4021 gatactcctt caacaccgac tcacgcacca ctggtgcagg cgcgtagata ggttgcacac
     4081 cttgcggatc gcttaaggtt tgcagcccca gcgccgctac cgggccgtca gtgccgtaag
     4141 ccattgcagc attaacgcca gagatttgct gggcagcggc tttgatcgtc accgccgtgt
     4201 cgccaccagc cagtgacagc aactgatcct gaccgagctt aaagccatag gctttttcaa
     4261 acgcgggtaa cgcatcggcg cgttcgataa actctgccga ggctgccagt ttgaaggtgc
     4321 cgccctcttt cagataacga ctcaggtcgg caagcgaagt gagtttattt ttctctgcca
     4381 catcctgacg cacggcgata gtccaggtgt tatttgcagg cgcgggcgtc agccagatta
     4441 acttgttgtg ctccgaatcg agctttttga ctttctcgta accttgctgc gcgtttttcc
     4501 acgctgcatc gttttcatct ttaaagaaga aagcgccatt gccggtatat tccggataga
     4561 tatccagttc accggaagta atcgccccgc gcaccacagg agtcgttccg agttgcactt
     4621 tattcaccgt cggtacgccg tggctttcca gcacctgcaa gatgatgttg ccgagtagcg
     4681 cgccttcggt atcgattttt gaaccgactt taacggggga agccgcctgt agcggcaggc
     4741 tcacggctgc caacataacc agtgaacctg cccagacctt tgagagtggc atgatgcttt
     4801 cctcattctt ttactgttgt tttcagcgaa ttaagagaaa agcatagttg ataatgacag
     4861 agttagcctg agcggaagga ttcagttgca gaatcagata aatacttaaa gggcatattt
     4921 tgcccggcgt ttcttgccgg atgcggcgta aacgccttat ccggcctaca aaggtcgaaa
     4981 attcagcaag ttaagatatg aacatcaggc aaagggcgca aacgtcgcgc ccttactaaa
     5041 gcattacagc aactcaaact cgcctttctt aacgcgtgcg gaatcagtgc cgataaagac
     5101 attgaacttg ccaggctcgg cgtcatattt catctgttga ttccagaact tcagcgcctc
     5161 gatatcaatc gggaagctga cagtctgagt ttcgcccggt ttcagggtga ttttctcaaa
     5221 gcctttcagc tgcttcactg gacggctcat ggaagccgtc acatcctgca agtacatctg
     5281 tactaccgtg gccccttcgc gtttaccggt gttcgtcacc tgcacgctgg ccgtcacttt
     5341 gccgtcacgc ttcatggtcg gcgcagaaag tttcacatca gagacggtga aagtggtata
     5401 gctcagacca tagccgaacg gataaagcgc cccgttagct tcatcaaaat aacgcgaagt
     5461 gtatttgttc ggcttgtcgg cattatacgg acgaccggtg ttcagatggc tgtagtacac
     5521 cgggatctgc ccgacagaac gcgggaagga catcggcagc ttgccggacg ggttgtaatc
     5581 gccaaacaac acatcggcaa ttgcattacc gccttcagtc cccgcaaacc aggtttccag
     5641 aatcgcatca gcctgctgat cttctttcac cagcgccagc ggacgcccgt tcatcagcac
     5701 cagcaccagc ggtttaccgg tggctttcag cgccgcaatc aagtcacgtt ggctttgcgg
     5761 aatagtgata tcggtacggc tggaggcctc gtgcgccatc ccctgtgctt caccgaccac
     5821 agccaccacc atatcagatt gtttcgccgt ctgcaccgct tcatcaatca tctcttgcgg
     5881 cgagcgcgga tcgactttga ccgcttcttc atactgattc aggaaatcga taatgccttt
     5941 gtcactggta acgttcgccc ctttggcata cagcacttta ccgttttcac cgacggcatt
     6001 tttaatcccg gtcagtacgg tcacggattg atcggcaaca ccggctgcgg accagctgcc
     6061 catcacgtcg cgtttactgt ccgccagtgg cccaaccacc gcaatggtgg ccgatttttt
     6121 cagcggtaac gtttcgagac ggtttttcag caacaccagg ctttcgcgcg ccacttcacg
     6181 tgcttcttta cggtgcaggc ggctttcggc attggtatcc accgggtcag actctttcgg
     6241 ccccaaatgg ctgtatgggt cgttaaacaa tcccatatca tatttaacgt tcagtacatg
     6301 gcgggcagca tcgtccagct cttccatcgt cactttgccg gatttgatca acccaggcag
     6361 atacttcgag taatactcgt cgctcatact catgttgatt ccggatttca gcgccacgcg
     6421 caccgcatct tccgggtctg ccgccgtgcc atgtttaatc agctctttga ttgcaccgtg
     6481 atcggaaacg gtgatgcctt taaagcccca ctggtcgcgc agaacatctt tcagcagcca
     6541 ggaatcggag gtggctggcg tgccgttcag cgagttcagc gccaccatca ccgcgccgct
     6601 gcctgcgtcc agccccgctt tgtacggcgg catataatca ttaaacaggc gctgcggact
     6661 catatcgacg gtgttgtact ctttaccgcc ttctaccgcg ccgtatgcgg caaagtgttt
     6721 gacgctggtc atcaccgagt agcgatctgc cgggctttta ccctgcatcg cttccaccat
     6781 ggttttaccc attgttgagg tgagatacgt atcttcgcca aaaccttcgg aagcacgtcc
     6841 ccagcgcgga tcgcgcgaga catcgaccat cggtgcccag gtcatattca ggccatcatc
     6901 tgccgcttca taagcagaga cacgtccgac cgttttcact gcatcgaggt taaaagacga
     6961 ggccagaccg aggctaatcg ggaacaccgt gcgctgaccg tgcagcacgt cgtaagcaaa
     7021 gaaaagagga attttcaggc ggcttaattc catcacctga tcctgcatgg cgcggatatc
     7081 ctgacgggtt acggtgttga aaatcgcccc aacctgaccg tctttgatca tctcgcggat
     7141 cgcctctttc gggttatccg ggccgacgct gattaagcgc agctgaccaa ttttctcatc
     7201 aactgtcatt ttcttaagca attcggtgac gaacgcatcc cgcgcttcgg gcgtgaatgg
     7261 atggttgccg aataaatcat ccgccagtgc tggctgcagg gccagactca ccgcgattcc
     7321 tactgaacat agccatttca tatggatttt tcttcctctg ttgcccgacg aggcagcgaa
     7381 aatggtgcaa aaaccgtagt ttgccataag catgatggag agagaaaaag aatgctcagt
     7441 ttattgtctg aattttcaaa atattcactc gctgaattgt catacaaggc gctattctag
     7501 tttgtgatat tttttcgcca ccacaaggag tggaaaatgt cttccatgac aacaactgat
     7561 aataaagcct ttttgaatga acttgcccgt ctggtcggtc attcacacct gctcaccgat
     7621 cccgcaaaaa cggcccgcta tcgcaagggc ttccgttctg gtcagggcga cgcgctggct
     7681 gtcgttttcc ctagctcact actggaattg tggcgggtgc tgaaagcctg tgtcaccgcc
     7741 gacaaaatta ttctgatgca ggctgccaat acaggcctga ccgaaggatc gacgccaaac
     7801 ggtaacgact atgatcgcga tatcgtgatc atcagcaccc tgcgtctcga caagctgcac
     7861 gttcttggca agggcgaaca ggtgctggcc tatccgggca ccacgctcta ttcgctggaa
     7921 aaagccctca aaccgctggg acgcgaaccg cactcagtga ttggatcatc gtgtataggc
     7981 gcatcggtca tcggcggtat ttgtaacaac tccggcggct cactggtgca acgtggcccg
     8041 gcgtataccg aaatgtcgtt attcgcgcgt ataaatgaag acggcaaact gacgctggtg
     8101 aaccatctgg ggattgatct gggcgaaacg ccggagcaga tccttagcaa gctggatgat
     8161 gatcgcatca aagatgacga tgtgcgtcac gatggtcgtc acgcccacga ttatgactat
     8221 gtccaccgcg ttcgtgatat tgaagccgac acgcccgcac gttataacgc cgatcctgat
     8281 cggttatttg aatcttcagg ttgcgccggg aagctggcgg tctttgcagt acgtctagat
     8341 accttcgaag cggaaaaaaa tcagcaggtg ttttatatcg gcaccaacca gccggaagtg
     8401 ctgaccgaaa tccgccgtca tattctggct aacttcgaaa atctgccggt tgccggggaa
     8461 tatatgcacc gggatatcta cgatattgcg gaaaaatacg gcaaagacac cttcctgatg
     8521 attgataagt taggcaccga caagatgccg ttcttcttta atctcaaggg acgcaccgat
     8581 gcgatgctgg agaaagtgaa attcttccgt ccgcatttta ctgaccgtgc gatgcaaaaa
     8641 ttcggtcacc tgttccccag ccatttaccg ccgcgcatga aaaactggcg cgataaatac
     8701 gagcatcatc tgctgttaaa aatggcgggc gatggcgtcg gtgaagccaa atcgtggctg
     8761 gtagattatt tcaaacaggc cgaaggcgat ttctttgtct gtacgccgga ggaaggcagc
     8821 aaagcgtttt tacaccgttt cgccgctgcg ggcgcagcaa ttcgttatca ggcggtgcat
     8881 tccgatgaag tcgaagacat tctggcgttg gatatcgctc tgcagcgtaa cgacaccgag
     8941 tggtatgagc atttaccgcc ggagatcgac agccagctgg tgcacaagct ctattacggc
     9001 cattttatgt gctatgtctt ccatcaggat tacatagtga aaaaaggcgt ggatgtgcat
     9061 gcgttgaaag aacagatgct ggaactgcta cagcagcgtg gcgcgcaata ccctgccgag
     9121 cataacgtcg gtcatttgta taaagcaccg gagacgttgc agaagtttta tcgcgagaac
     9181 gatccgacca acagtatgaa tccggggatc ggtaaaacca gtaagcggaa aaactggcag
     9241 gaagtggagt aaaaattacg gatggcatag tatcgccatc cgtcattcac ttaatcgttc
     9301 tgtgccgtct gccccgccgc cgccatttgg gcggcttttt gttttttata gctcaacgct
     9361 gctgctggaa caggcatcac tttaccggtt tcaatccagg tacgcaggcg gctggcatcg
     9421 gcaaaatggg tatatttgcc aaacgcatcc atcactacca gcgccaccgg tttattattg
     9481 ataaccgtac gcatcaccag acaatggccc gccgcattgg taaaaccggt tttggttaac
     9541 tgaatattcc agttatcgcg atacaccaga tggttagtat tgcggaacgg cagcgtatac
     9601 gtcgggttag agaaggttgc catatcttcc cgggtagtac ttaactgccc gatcaacgga
     9661 tattgtttgc tggcaatgag cagtttggtt aagtcacggg cagttgaaac gttatgcacc
     9721 gacaatccgg taggttcaac aaagcgcgtg ttgttcattc cgagcgattt cgctttcgca
     9781 ttcattgcct taataaaggc tttgtaacca ccgggataat ggtgtgcaag gcttgccgcc
     9841 gcgcggtttt ctgaagacat cagcgccagc aacagcatat ctttacggct gatttcgcta
     9901 ttcagtcgta cgcgcgaata gacccctttc atctccggcg tctggctgat atccactttt
     9961 agtttttcat ccagcggcag tcgtgcatcc agcacaacca tcgcggtcat taatttgctg
    10021 atagacgcaa tcggacgcac cagatccggg tggttcgagt agatcacttt gttggtattc
    10081 agatcaacaa tcatcgcgct accggaggca atttcaggtt gtgaagcggt gatagcggct
    10141 gccgttttcg caacggcctg cggtgccaaa ggcacagcca gcatcagggc caggctaaat
    10201 aaagaaactc gaaatttcgg catgatgagc attcagatag tggttcacgc gcacaggttg
    10261 cgcaccgccg gagtaaggat ttactgaggc tagcgacgcc atcataacga gcaaaaagtg
    10321 cgatcgtcaa aggagaatcg tgaggaaatg ctgcattgct gacatttacg ccagcaatgc
    10381 aacgtcaaag aactttctag aacaaacgat aaccgtagcc ccatagtata acggttaggg
    10441 cgagcagcac ttccagtacc agcacgccaa tcgccagcgt cgaactagag aagctaaggc
    10501 cttcctcttt gttgatattc aggaagctcg gaataccaag gtaaagcagg tagccggtgt
    10561 aaaacagcgc caccgtgccg accagcgcac acaaccagac cagtggataa agcgccacca
    10621 aaccgcttaa aaacagcgga gttgcaacgt agcccgcgaa gaccatgcag tgcgcaagtg
    10681 acggacgctg cggataatta cgcgccatcc accagatgac ccgccccatt accgcaaccc
    10741 cagccagcat aacgccataa aacaggacag ccagcgccag tccggtaaac caggataact
    10801 tcaggatagt gccatcgcca aaattccagc caatctgtgt agtgccaatg aaggcgcaaa
    10861 tcaccggaat cgccgccatc agcaaaacgt ggtgggtgta atggtgagaa atcgtttcgt
    10921 tttcgcgatt aatcacctgc atttcacgat cgggatggga aaacagtccc cagacatggc
    10981 tcataccgcc cccttgttgt gagttcatga acctgacagt tcaagtataa gtcagcttgt
    11041 gattattttt tgttcgctca tgtaatttgc accgtctgat gtacgtcctt tttcagcgtg
    11101 aatgatttcc agggtgtatg cttacaggca accaaaggga gacagactgg cctatggatc
    11161 tcaatacact catctcacaa tatggttatg ccgcgctggt gatcggtagc ctggcggaag
    11221 gtgaaaccgt gactttgctg ggaggcgttg cggcacatca ggggctatta aagttcccgc
    11281 tggtggtact ttctgtggcg cttggcggca tgattggcga ccaggtgctt tatctgtgcg
    11341 ggcggcggtt tggcggcaag ctgttacgcc gtttctcgaa acatcaggat aaaattgagc
    11401 gggcgcagaa acttatccaa cgccatccgt atctgtttgt cattggtacg cgctttatgt
    11461 atggctttcg ggtgattggc ccgacgctga ttggtgccag ccagctgccg ccgaaaatct
    11521 ttctgccgct gaatattctc ggcgcatttg cctgggcgtt gatttttacc actattggtt
    11581 acgctggtgg tcaggtgatt gcgccgtggt tgcacaatct cgaccagcat ttgaagcact
    11641 gggtctggtt gattctggtc gtggttctgg tggtgggcgt gcgctggtgg ctgaaacggc
    11701 gcgggaagaa aaagccggat catcaggcgt aaaaccattg ccctgggtaa ggcgttcgcg
    11761 ccgcatccga catccggtgc accatgcctg atgcgacgct tgtgcgtctt atcatgccta
    11821 ccaaaatttt acccattgaa ataaccacgc acctcactgc gctattctgg gttgaactgc
    11881 ggattcgcca acataaagcc gccatccact atcaacgact gcccggtggt gtaatttgcg
    11941 ccctctgagc acagccacgc taccaggctg gcaatctcat gcgttgcgcc aaaacgccgc
    12001 agaggaatcg aaggctccgc gtcgggcttc acgtcgctgt catccatgcc attcattggc
    12061 gtggcgatcg ccccaggcgc gactgcgttc accaaaatct tatgcctgac cagctccagc
    12121 gccatcgctt tggttaaccc accgagcgca tgtttagcgg ctgtgtaggc gctggcatcc
    12181 ggcagcggcg tatgttcatg taccgatgta atgttgatga tgcgaccgcc ctgcccttgt
    12241 ttcaccatct ggcgggccgc aatttgtgag cataaaaatg caccatcgac atcaacggta
    12301 aaaatcttgc gccactcatc gaaagccata tcaagaaacg gcgctttggt cattgcgccc
    12361 gcattattca ccagcacatc aatacgcccc agccgttgaa tgagtttctc cagcgcctgc
    12421 gcaccttctg gcagtttacc gagatccagc tgcacgatct ccgcacgtac gccgtggcta
    12481 actacctcac gcgcggtatc ttttgcccct tcttcatctg agtgccaggt aatgccaata
    12541 tcaaaccctt gctgcgccag taataacgcg cactctttgc cgatccccga atcggaggcg
    12601 gtaataatcg caacctgtgc catcgagttc tccacttaac gctgaataaa cgttaagtat
    12661 agaaggcgca tatcatcagc gtttgtaacc cccgcccaac gcaccagtga gttgaatgga
    12721 ggcatccagc cactgccctt gcaataacag gccattggcc cgctcacgca gcgcggggat
    12781 tctggcttcg ctgacgcggg aaccagcaat gatgcccgcg ttaaagcgcg cctgcgcaag
    12841 acctaccaca cgcaaggtat cgcgctcaat ttgcgcctga tgctggtttt tctccgcaag
    12901 tgtctgaacc tgactggctg cccgcgccac gtcattcacc gcttcaacca ccgctttgtt
    12961 gtagctggcg atagacaagt tgctttcggc ttttgcgata tcgagattgg cgttaagacg
    13021 accactatcg aaaatgggta gcgtcaggcc tgccgtaacg cccatttgct gcgcggaatg
    13081 acggaacaga tcgcttaagt gcaacgcatc ctgttgcagg aaggccatca ggttgatgtc
    13141 aggataaaat gccgcttttg ccgcatcaat ggtgcttagc gatgactcaa cgtaccagtg
    13201 cgccgcctgc aaatctgccc gccgggccag taaggagtac cccagttcat caggaagctg
    13261 gcttgccact ttcggcaacg cgaccggttt aagcttcaat gactttgtct ggttatttgt
    13321 aagtgcgctt aaccgtgcct caataatttt cattttcccc gcgacatcgt tgagctgctg
    13381 ccgggttttg ctggcattaa tatcggtttc cacaccttca actgaagaag taatcccgtt
    13441 ctgatatagc tggcgatcgg tcgcgataat ggtgttctgc tctttttcta tttgctgcaa
    13501 gaccgtgttt aacgccgcct gg
//
LOCUS       DAZPEB010000108        13480 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZPEB010000108 DAZPEB010000000
VERSION     DAZPEB010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13480
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.109"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(103..3324)
                     /gene="carB"
                     /locus_tag="ACHURJ_002676"
     CDS             complement(103..3324)
                     /gene="carB"
                     /locus_tag="ACHURJ_002676"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFR1281563.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(3342..4490)
                     /gene="carA"
                     /locus_tag="ACHURJ_002677"
     CDS             complement(3342..4490)
                     /gene="carA"
                     /locus_tag="ACHURJ_002677"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFR1281564.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
     gene            complement(4945..5766)
                     /gene="dapB"
                     /locus_tag="ACHURJ_002678"
     CDS             complement(4945..5766)
                     /gene="dapB"
                     /locus_tag="ACHURJ_002678"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFR1281565.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSRESHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNSL"
     gene            complement(5933..6847)
                     /gene="rihC"
                     /locus_tag="ACHURJ_002679"
     CDS             complement(5933..6847)
                     /gene="rihC"
                     /locus_tag="ACHURJ_002679"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFR1281566.1"
                     /translation="MHLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMSGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPELFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVKVALDLDVKGFQQWVAEVLALAS"
     gene            complement(6913..7863)
                     /gene="ispH"
                     /locus_tag="ACHURJ_002680"
                     /gene_synonym="lytB"
     CDS             complement(6913..7863)
                     /gene="ispH"
                     /locus_tag="ACHURJ_002680"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFR1281567.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(7865..8314)
                     /gene="fkpB"
                     /locus_tag="ACHURJ_002681"
     CDS             complement(7865..8314)
                     /gene="fkpB"
                     /locus_tag="ACHURJ_002681"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR1281568.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(8383..8877)
                     /gene="lspA"
                     /locus_tag="ACHURJ_002682"
     CDS             complement(8383..8877)
                     /gene="lspA"
                     /locus_tag="ACHURJ_002682"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFR1281569.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRLKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSR
                     AKKQ"
     gene            complement(8877..11693)
                     /gene="ileS"
                     /locus_tag="ACHURJ_002683"
     CDS             complement(8877..11693)
                     /gene="ileS"
                     /locus_tag="ACHURJ_002683"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFR1281570.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGNPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHFSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYHDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(11736..12677)
                     /gene="ribF"
                     /locus_tag="ACHURJ_002684"
     CDS             complement(11736..12677)
                     /gene="ribF"
                     /locus_tag="ACHURJ_002684"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFR1281571.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYVVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(12685..12903)
                     /locus_tag="ACHURJ_002685"
     CDS             complement(12685..12903)
                     /locus_tag="ACHURJ_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFR1281572.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            13006..13269
                     /gene="rpsT"
                     /locus_tag="ACHURJ_002686"
     CDS             13006..13269
                     /gene="rpsT"
                     /locus_tag="ACHURJ_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFR1281573.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT         3150 a         3804 c         3399 g         3127 t
ORIGIN      
        1 caaaagattt acttaatggg caaaaaaaac ggtcctcatc agagaaccgt attacttatt
       61 cgatcaaatt agcggatgaa aaatatccgc catgacacgc tattatttga tctgcgcgtg
      121 catttcctgc accgaaatta ctttttcggt cgcgtcggca ttcagcgcca tcgcggtagc
      181 gaaaccaccg ttcagggtgg tgtcataatg cactttatat tgcagcgcac tgcgacggat
      241 cacgcgggag tcttcaatcg cgcgacggcc tgaagtggtg ttgatgatgt aggtatattc
      301 gccattcttg atgcggtcct gaatgtgcgg acggccttca tgcaccttgt tcaccagacg
      361 cggattaatg cccgcttcgc ccagcacaat cgccgtgccg tgggtcgcat ccagctcgaa
      421 gccctgtttc agcagttttg ccgccaggtc caccacgcgt tctttatcgc cttcgcgcac
      481 ggaaagcaac gcacgaccgt gtttcttcat ggtggagttg ctgcccagct gcgctttggc
      541 aaacgcttca gcgaaggtgc ggcccacgcc catcacttcc ccggtggagc gcatttctgg
      601 ccctaacagc gggtcaacac ccgggaattt attgaacggc agcaccactt ctttcaccga
      661 gtagtacggc gggataactt ctttggttac gccctgctca gccagcgatt tgccagccat
      721 cacacgcgcc gccacttttg ccagcggtac gccggtggct ttggagacga atggaacggt
      781 acgcgccgca cgcgggttaa cttcaatcag gtagacttcg ttgtttttca ccgcaaactg
      841 cacgttcatc agaccgcgca cctgcaattc gaaggccagt ttctgcacct gctggcgcat
      901 cacatcctga atttcctgac ttaaggtgta ggctggcaga gaacatgcgg agtcaccgga
      961 gtgcacgccc gcctgctcga tgtgctccat gatgccgcca atcagcacca tttcgccgtc
     1021 gcagatggca tccacgtcaa cttctaccgc gtcatcaagg aagtgatcca gcaacactgg
     1081 cgcatcgtta gacacgctga ccgccgtctg gaagtagcga cgcaggtcag cttcgtcata
     1141 gacgatttcc atcgcccgac cgccgagaac gtaagacgga cgtaccacca gcgggtagcc
     1201 aatctctttc gccttctcta ccgccatttc aatagcggta acggtggcgt tcgccggttg
     1261 tttcagtttc agacgctcaa ccgcatgctg gaagcgttca cggtcttctg cacggtcgat
     1321 agcatccggg ctggtgccga taaccggtac gccagcagct tccagcgcgc gcgccagttt
     1381 cagcggggtc tgaccgccgt actgaacgat aacgcctttc ggcttctcaa tacgcacgat
     1441 ttccagcaca tcttccagag ttaccggctc gaagtagagg cgatcggaag tgtcgtagtc
     1501 ggtggagacg gtttccgggt tacagttaac cataatggtt tcgtaaccgt cttcgcgcag
     1561 cgccagcgag gcgtgtacac agcagtagtc gaattcgata ccctgaccga tacggttcgg
     1621 gccgccgccg agaaccatga ttttttcacg gtcggtagac ggattcgctt cgcactcttc
     1681 ttcataagtg gagtacatgt aagcggtgtc ggtggcgaac tctgccgcac aggtatccac
     1741 gcgcttataa accgggtgca ggtcatactg gtcgcgcagc ttgcggattt ccgcttcgcg
     1801 cacgcccgcc agttttgcca gacgcgcatc ggcaaagcct ttgcgtttca gctggcgcag
     1861 gaattcagcg ttcaggccag tgatgcccac ttccgctact ttctcttcca gacgcaccag
     1921 ttcttcaatc tgtaccagga accagcggtc aatattggtc aggttgaaca cgccgtccac
     1981 agacaggccc gcacggaagg catcggcgat gtaccagata cgctctgcgc cagcatcttt
     2041 cagttcgcga cggattttgg ttaacgcttc cgggtcatcg aggctcactt tcgggtcgaa
     2101 tccagtcgca ccgacttcca ggccgcgcag cgctttttgc agggattcct gctgcgtgcg
     2161 accaatcgcc atcacttcgc caaccgattt catctgagtg gtcagacggt cgttagcacc
     2221 agcgaatttt tcgaagttga agcgaggaat tttagtaacc acatagtcga tggacggctc
     2281 gaaggaggcc ggagtacgtc cgccagtgat gtcgttcatc agttcgtcga gggtgtaacc
     2341 caccgccagt ttcgccgcca ctttagcaat cgggaaaccg gtcgctttcg acgccagcgc
     2401 cgaagaacgg gacacgcgtg ggttcatttc gataacaatc aggcgaccgt ttttcgggtt
     2461 caccgcaaac tggacattgg aaccaccggt ttcaacgccg atttcacgca gcaccgccat
     2521 cgaggcgtta cgcatgattt gatattcttt gtcggtcagc gtttgggctg gcgcgacagt
     2581 gatggagtca ccggtatgga tgcccatcgc atcaaagttt tcgatagagc agacgatgat
     2641 gcagttgtcg tttttatcac gcaccacttc catctcgtac tctttccagc cgatcagcga
     2701 ctcatcaatc agcagctctt tggttgggga gagatccaga ccgcgggcgc aaatttcttc
     2761 aaactcttcg cggttataag cgataccgcc gccgctaccg cccatggtaa aggatgggcg
     2821 aataatgcac gggaagccca cgtcagcggc aaccgccagc gcttcttcca tcgtgtgtgc
     2881 gataccggaa cgcgcggttt ccagaccaat tttcttcatc gctacgtcga aacgacggcg
     2941 gtcttctgct ttatcaatcg catcggcggt cgcaccaatc atagtcacgc cgaactcttc
     3001 caacacgccc tgacgctcca gctccagagc gcagttcagc gccgtctgac cgcccatcgt
     3061 tggcagcacc gcgtccgggc gctctttttc aataatcttg cgtactactt cccagtgaat
     3121 cggctcgatg taggtcgcat cggccatttc cgggtcggtc atgatggtcg ccgggttgga
     3181 gttcaccaga atgacgcggt aaccctcttc gcgcagggct ttacatgctt gcgcgccaga
     3241 gtagtcaaac tcacacgcct gaccgataac aatcgggccc gcacccagaa tcaggatact
     3301 ttttatatct gtacgttttg gcatggctct tttactcctg attacttagc ggttttacgg
     3361 tactgctcaa ttaactcgat aaagtggtcg aacaacggcg ctgcgtcgtg tggcccaggg
     3421 ctggcttccg ggtgaccctg gaagctgaac gccggtttat cagtgcgatg aatgccctgt
     3481 aacgtaccgt cgaacaggga tttatgcgtg acacgcaggt ttgcaggtaa tgttgcttca
     3541 tccaccgcaa aaccgtggtt ctgggcggtg atcatcacca cgtttttctc cacatcttta
     3601 accggatggt tgccgccgtg gtgaccaaat ttcattttga cagtcttcgc accgctcgcc
     3661 agcgccagca gctgatgacc gaggcagatg ccgaataccg gaatatcggt ttcgaggaat
     3721 ttctggatgg cggtaatggc gtaatcgcac ggggccgggt cgccaggacc gttggagagg
     3781 aagatgccgt ctggattcat tttcagcaca tcttccgcag aagtttgcgc cggaacgatg
     3841 gtcaggcgac agcctctatc caccagcatc cgcaggatgt tgcgcttggc accaaaatca
     3901 taagccacga cgtggaacgg cagctcgtct tcttttttcg cttctggcag gccgccggtc
     3961 aacgtccagc tcccttgtgt ccagctatag gcttctgcgg tggtcacttc ttttgccaga
     4021 tccatgccat tcagacctgg gaacgcgcgg gctttttcta acgccaacgc cgcatccggg
     4081 ttatcgcctg cgataatgca gccattctgt gcgcctttct cgcgcagtaa acgcgtcagc
     4141 ttacgggtat cgatatcggc aatcgccacg atgttatggc gcttcaggta agaagagagg
     4201 tcttcggtat tacggaagtt gctggcaatc agcggcaggt cgcgaatcac cagaccttgt
     4261 gcatgtacct gagaagattc ttcatcggcg tcattagtgc cgacattgcc aatatgggga
     4321 taagtaagag taacgatttg acgagaatag gaaggatcag tgaggatttc ttgataaccg
     4381 gtcattgaag tattgaaaac gacttcccca accgccgaac ctgttgcccc tatggcccga
     4441 ccgtgaaact gggttccgtc ttccagaacc aatagcgctg acttaatcaa aacaccctcc
     4501 agagaatatt cactcacttt atttgcatat taattcacaa tgatgacgta aatcaatgca
     4561 aatctgctta ccgacgaatt tctggcaaac ggcggcattc tggagatatg ggcgctaaaa
     4621 gtcaacttaa tggcgggatt ttttatatta ttttgtggct catgcatgtt ttacaggcat
     4681 taagcgacaa aaagatcata taacctgttc tggaaaacgc ttgcacagca agaagattgc
     4741 atgaaataaa agggagcagg gaaaagtgga ccaaatggtc aaattttaca ttaaaacaac
     4801 aaaaacagaa gacaaaacat gtaatttttg gcacttatag acaatttaaa aacataacac
     4861 taaaaataaa agggccaaaa ttaaagccct ttataatcaa taaattaaat gtgttatttt
     4921 tgcaccataa caaatatttt gtggttacaa actattgaga tcaagcacat ctcgcatatc
     4981 aaaaagacca ctttccttac cactcaacca caaagccgat cttaccgcgc cgttagcaaa
     5041 tgtcatgcgg ctggacgcct tatgggtgat ctccagacgc tcgccaatat cggcaaacat
     5101 cgcggtatgt tcgccaacga tgtcacctgc acgcacggtg gcaaaaccaa tggtgccagg
     5161 cacacgttca ccggtgtggc tttcacgact gtagaccgcg caatctttca gatctttatc
     5221 aagggcgtgg gcgatcgcct ctcccattgc cagtgcggtg cctgacggcg catcaacttt
     5281 atgtctatga tgtgcttcaa taatttcgat atcggtgtag tcacccatca ctttggctgc
     5341 tttctccagc agcttaagca tgacgttaac gccaacgcta aaattggcgg caaagacaat
     5401 cgcaatatcg gcggcagcgt cacgaatcgc ctgtttaccg gcttcgtcaa accccgtagt
     5461 gccgatcacc atccctttgc catgctggcg acaaaaagcg agatggttca gcgtaccttc
     5521 cggacgggta aaatcgataa acacatcaaa atcatctttt accgcatcga ggctgctttg
     5581 cacggtaacg cctgttttcc ctgcaccagc cagctcaccg gcgtcgctgc ccagtaaaga
     5641 agatccttcg cgctccagcg ccgcgcccag ctgcacgcct tctaatgcca gcgccgcctg
     5701 aatcaactgg cggcccatac gccccccggc tcccgcgatg gcaacgcgga tgtttgcatc
     5761 atgcatagct attctctttt gttaatttgc atagaccgtt ttcagagtaa ccagcgcatc
     5821 gctacgccgc cagccaaaaa cgctgataat tagaaattaa tgataaacag ggagggatat
     5881 cagtgaaagg catgagtcga tgaatgactg catgccgata acatgtgaca ggttacgacg
     5941 ccagagccag cacctcagcc acccactgct ggaagccttt cacatccaga tccaatgcca
     6001 cctttacatt ggctggtttg cccaggcaac cgtcgatatc aaccaccgtc gtgcctgagg
     6061 taaattcgcc ctgagtttcc actgccacaa aacacggttt gagagtgaac aattccgggc
     6121 gcaccagcca ggcaatagcg caaagatcgt gcatacgcag tccactttgc atactgccgc
     6181 tacggtagtg gctaaacagg gcgtggagca tttttccggt gcgattcaac tccggcaaag
     6241 tagcgagata gtcaggggtt aatattgcct gattggtgac atccagaccg ctcatgacga
     6301 tttcgatgcc gctacggaag acacaggcag ccgcttctgg atcggcagcg atattaaact
     6361 cggcgtttgg tgtgcagttg ccgcgaccag cagaaccgcc catgatcacc agacgacgaa
     6421 tatacggttt acattccggg cattgtgaaa gtaacagcgc aatattggtt aacgggccga
     6481 tggccaccag ggtaacgggc tctggtgcac gcatcagggc atcccgaatc gccagaaacg
     6541 ctggtatccc gagcggcttt cggttgtgct caacaaagtc gtagccagcc attcccgatt
     6601 cgccgtgcac agatgccgca tcacgcggtg cgcgtaccag cggcacagcg gctccctggg
     6661 cgagcggaat ctccgcattc cagaaatgca gcagttgcag ggcgttgcgg gtggttttct
     6721 caaccgagac attacccgcg acggtggtca tcagttgcag gtcgagttcg ggtgcaaaaa
     6781 tcgcggcggc aatggcgacg gcatcgtcaa tgccggggtc agtatcgagg aagataggta
     6841 aatgcatgtt ttctccataa aaaatgccgg taacaagacc ggcattttcg cataacttag
     6901 gctgctaatg acttaatcga cttcacgaat atcgacacgc agctctttcg gcacttcgaa
     6961 aacaatgttt tcttcacggc cttccagcgg aatggcttca ccaccgccca gctgctgcaa
     7021 acgtgccacc acattctgca ccagaatatc cggagccgat gcgcccgcag tcacgccgac
     7081 gcatttaacc tctttcaccc actcttcctg gatgtctttc gcatcgtcaa tcaaaaacgc
     7141 gcgtttgccc atacgctggg ccagctccgc cagacggttg gagttggagg agtttttcga
     7201 accgaccacc aacacaactt ccgcctgttc tgccagggcg cgtaccgctt cctgacggtt
     7261 agtcgtggcg tagcagatgt catctttgcg cggaccgaca attttcggga agcgtttacg
     7321 cagcgcgtcg atcacatcag acgtgtcatc caccgacagc gtggtctggg tcataaagga
     7381 gagcttctct tcgtttttga ccgtcagttt ccacacatcg tccggcgatt cgaccagata
     7441 cattccccct tccgggttac tgtactggcc cattgtccct tccacttccg ggtgcccggc
     7501 gtgaccgatg agaatagatt cttcgccacg gcgactggcg cgggcgactt ccatatgcac
     7561 tttggtcacc agcggacagg tcgcgtcaaa taccgtcaaa tcgcgacttt ttgcttcgtt
     7621 acgtaccgcc tgagaaacac cgtgtgcgga gaaaatcagg atcgcgccgt ccggtacttc
     7681 gctaatctgc tcaataaaga tagccccacg ctcacgcaag ctatcgacca catagcggtt
     7741 atgtaccact tcgtgacgga catatatcgg tgcgccgtaa atggccagcg cgttttcaac
     7801 aatgctgata gcgcggtcta ccccggcaca aaaaccacgc gggttggcca acaggatctg
     7861 catgttacgc ctccaatgcc ggatcgattt ccagcacttc aatatcaaaa tgaacggtct
     7921 gcccggccag aggatggttg aaatcaacgg taatggagtc gccgttaatt tcgcggatca
     7981 cgccaggcat ctcactgcca tccattgcgg taaaaagcat gattgcgcca atttctggct
     8041 cgcctgcatc cataaattca cggcgggaga agtactgaat caggtccggt gacggcacgc
     8101 caaacgccgc atctggctcc aacgagaagg tggttttatc gcccactttc agccccaaca
     8161 ggtgttgctc cagcccttca gaaagagaag catcacccag gcggaacagc gccggtttac
     8221 cgttgttgcg ggtagactcg gcggtggtgc catcgtcgag ttttagcgtg aagtgcacca
     8281 ggacggcgct attgctctgt acagattcag acatgcaggt ttgctcttat atttgaggat
     8341 ttttgccgga tgcggcgtaa acgccttatc cggcagggtt tattattgtt ttttcgctct
     8401 agaaggcaaa aaaccttcca gcacaatcag tgccgcaccg acacagatgg cagtatcggc
     8461 aaggttgaag gtggcgaagt gccagtcgcc gacgtagaag tcgatcatat cgacaacgaa
     8521 gccgtgccac aggcggtcga acaggttgcc cagcgcgccg ccaataatca gcgcgtaagc
     8581 gatattgttt agcttctgcg tggccttcaa gcgatacatc atcactgcca ggatcacgct
     8641 aataccaatc gcaataccgg caaagaacca acgctgccag ccgccgctat cggcaaggaa
     8701 actaaacgcc gcgccatagt tacgcgcata atgcagatta agcgacggga acagcgggac
     8761 cgtatccccc agagcaaagt tctggaggat caggtatttg ctgcccagat cgataatcag
     8821 cacgactacc accagccaca gccagcgtag ccctgttgaa cagatcgatt gactcatcag
     8881 gcaaacttac gtttttcacc gtcaccggcg acgttgctga cacagcggcc gcagatttct
     8941 gcgtgttccg ccaccttgcc gacatcctgg gtgtagtgcc agcagcgtgg gcacttctca
     9001 ccttcggctt tactcaacgc gactttcagc cctttgagta cttcgctctg ctgagcatca
     9061 gcaggtgcgt cgtgatagtc tgcaacggta gcgccggagg tcaacaggac aaatcgtaat
     9121 tcatcgccca gcgcggtcag tttcgccgcc agttccggtt ctgcatacaa ggttactgcc
     9181 gcttccagcg agccacccac tttcttgtcg gcacgcgctt gctcaatgac tttgttcact
     9241 tcgccacgca ctttcaacag ctcgtcccag aacgcatcgt tcatcgcttc gctgtctgcc
     9301 aggccaaaca ggccttcgta ccactcaccg gtgaagacgt atttttcacg ttcgcccggc
     9361 aggtagcccc acacttcatc agcggtgaag gagaggattg gtgccatcca gcgcaccagc
     9421 gcttctgcga tgtgatacag cgcagtctgg cagctacgac gcgccacact gtccgctttg
     9481 gcggtgtact gacggtcttt gatgatgtcg aggtagaagg aacccatctc aacggagcag
     9541 aagcgcatca gacgctgtac cacttcgtgg aaatcgtatg cttcgtacgc cttgaggatg
     9601 tcttcctgtg ccgctttcgc acaacctacg gcccagcgat ccagtaccac catctcttcc
     9661 ggtttcacca tatcttttgc tggatcaaaa ccgttcaggt ttgccagcag gaagcgcgcg
     9721 gtgttacgga tacgacgata gctatcggca gcacgtttca ggatctcgtc agaaacggcc
     9781 atttcaccgg tgtagtcggt tgatgccacc cacagacgca gaatatccgc gcccagtttg
     9841 ttcatcacat cctgcggcga aacggtattg ccgatggatt tagacatctt gcggccctga
     9901 ccatccacgg taaagccgtg ggtcagtacc tgacgatacg gcgctttgcc tttcatcgcg
     9961 gtggagatca ttagggaaga catgaaccaa ccacggtgct ggtcagaacc ttccagatac
    10021 atgtccgctg cgtgaccggc aaattccgga cgcacgtcaa caacagaaaa gtgggtagat
    10081 ccggagtcaa accatacatc caatgtgtcc ggcactttca cgtactgatc agcttcatcg
    10141 ccgaggatct ctttcgcatc aagatcccac cacgcctgga tgccgtcaac ttcaacgcgt
    10201 tttgccactt cttccattag ttcgagagta cgcggatgca gctcttccgt gtctttgtgc
    10261 acgaacagtg acatcggtac gccccaggtg cgctgacggg agatacacca gtcaggacgg
    10321 ttagcaacca tcgactcgat acgcgcctgg ccccagtccg ggatccactg cacgcctttg
    10381 atctctttca gtgactgcgc acgcagacct ttctgatcca tgctgacgaa ccactgcggc
    10441 gtcgcgcgga agatgatcgg cgttttgtga cgccagcagc acggatagct gtgctgcatt
    10501 ttctcaacgt gcagcagcgc gcctttttcc tgcagcagcg caacgacgat gtcgttcgct
    10561 ttgaagacgt tcacgccatc cagcgtcgga taagtgcccg gcagataagt gccgtccggg
    10621 ccaaccgggt tagcggtttc caggccgtat ttctgaccga tcacatagtc gtccgggccg
    10681 tggccaggcg cggtgtgaac ggcaccggta ccggcatcca gggtaacgtg atcgccgagg
    10741 attgccggaa cgtcgaagcc cataaacgga tgggtaaagc gcagcaactc aagctccgca
    10801 ccttttaccg tgccgagaat ggtgtaatcg gtcacgccga tacgctgcat tacgctttca
    10861 accagatctt tcgccagaat cacggcctga ccgtcgatct gcaccagcgc atagtcgaaa
    10921 tcaggtgcaa tagagattgc gcggttcgca ggcagagtcc acggcgtagt ggtccagatc
    10981 accagcgaga ttgggccgtt aacgttgctt acggcaaatt ttgctttcag tgcatcctga
    11041 tcgaccgcct gaaaagcaac gtcgatggac ggagaagttt tgtcgtaata ctcaacttcc
    11101 gcttccgcca gcgcagaacg gcagtcaacg caccagtgta ccggcttcgc gcctttgtgc
    11161 aggtgaccgt tgccgatgat tttgcccagc gcgcggatga tgttggcttc agttttgaag
    11221 tccatggtca ggtacgggtg cgaccagtcg cccagcacgc ccagacggat aaagtctttg
    11281 cgttgaccgt caacctgggt cgccgcgtat tcgcggcact tggcgcggaa ctcggcggcg
    11341 gtgaatttct cacccggatt accgtattct tgctctactt tcagctcgat cggcagaccg
    11401 tggcagtccc agccaggcac atacggcgag tcataaccgg aaagcccttt ggacttcacg
    11461 ataatgtctt tcagaatctt gttaaccgag tgaccaatat gaatgctgcc attcgcataa
    11521 ggagggccat catgcagaat gaaggttttt ttgccttttt tagccgcacg gatgatgccg
    11581 tacagatcat catcagtcca acgcgccagc attcccggtt cgcgcttggc gagatcgcca
    11641 cgcatcggga accctgtttc cggcaaattc agggttgatt tatagtcact catcagattc
    11701 tcggttccgt gtttcggttt gattacataa caggcttaag ccggttttgt tagcccaaaa
    11761 aattcgcggg cggttaattc atcacgcgca atctgcgctt tcagttcgtc cagcgacgca
    11821 aatcgctgct cattgcgtat ttttttacgc agcactactt gtatatggcg accgtaaagg
    11881 tccattgcaa catctaacaa atgcacttcc agctgctggc gaataccggc aaccgttggg
    11941 cgtgttccga tgtttgccac gccgggtaac ggcttttcac cgaggcccag cacttctacc
    12001 acataaaccc ctttcaccgg ggaaacctga cggcgtagcg gtacattcgc cgtcgggaaa
    12061 cctatagtgc gccctaattc atcaccgtgg actacacgcc cggagatggc aaacgggtgc
    12121 cccagtaaac tttctgccag agccagattg tcatccgcca gcgcctgacg cacagcggtg
    12181 ctgctaatac gcacgccacc ttcgcaaaaa gtttgcgtac tagtgatatc gaagccgtat
    12241 tccatgccag ctttctgtaa taacaagaaa tcgccttcac gaccagcgcc aaagcggaaa
    12301 tcatccccta cggcaagaaa ttttacgcgc aaatgcttca ccagaagatc gctgatgaaa
    12361 ttttgcgcgg ttaacgccgc gaaacgcctg tcgaaacgca cgcacagcac gtaatcaacg
    12421 ccacactctg caaggtaacg cagtttttcc cgcagccggg tcagtcttgc cggggcttta
    12481 tcggtagcaa acagttccag tggttgaggt tcaaaaagca tcaccatcac cggtaagttg
    12541 cgcttgcgcc cttcttcctg caagccctgt aacagcgcgc gatgaccgcg atgcacgccg
    12601 tcgaaattac caatagtcag cacacaccct tcttgcgggg cctggctgag attatgtatg
    12661 ccgcgtatca gcttcatgtc tggctcaaaa cagtgaaaat cgtccgagta taccttgtac
    12721 agcggtcaag gttaaccggc gattgagtac cgaaatcaga aagaagtggc ttttcattgc
    12781 catggcgcaa atcacgggaa gaaagtgacc gcctgctgca atttttatcg cggaaaagct
    12841 gtattcacac cccgcaagct ggtagaatcc tgcgccatca ctacgtaacg agtgccggca
    12901 cattaacggc gcttatttgc acaaatccat tgacaaaaga aggctaaaag ggcatattcc
    12961 tcggcctttg aattgtccat atagaacaca tttgggagtt ggaccttggc taatatcaaa
    13021 tcagctaaga agcgcgccat tcagtctgaa aaggctcgta agcacaacgc aagccgtcgc
    13081 tctatgatgc gtactttcat caagaaagta tacgcagcta tcgaagctgg cgacaaagct
    13141 gctgcacaga aagcatttaa cgaaatgcaa ccgatcgtgg accgtcaggc tgctaaaggt
    13201 ctgatccaca aaaacaaagc tgcacgtcat aaggctaacc tgactgcaca gatcaacaaa
    13261 ctggcttaat cgccaatttg ctgaagcttt gtgaaaaagc ccgcgcaagc gggttttttt
    13321 atgcctgctg cttttatgat gcgtgagaga acacactacc ggttgccaga tagtcaaaag
    13381 atgctggctt ttgcagacac gccgttacac tttctgaact cttcgtggct taagaatcgt
    13441 agaaccatcg gctgatacat tctgaacaag aatatctact
//
LOCUS       DAZPEB010000109        13432 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZPEB010000109 DAZPEB010000000
VERSION     DAZPEB010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13432
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.110"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..310
                     /locus_tag="ACHURJ_002687"
     CDS             <1..310
                     /locus_tag="ACHURJ_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFR1281574.1"
                     /translation="VVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINGLQF
                     FDQQIRWAKADLVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAILLIN
                     "
     gene            509..790
                     /gene="ppiC"
                     /locus_tag="ACHURJ_002688"
     CDS             509..790
                     /gene="ppiC"
                     /locus_tag="ACHURJ_002688"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFR1281575.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(877..2352)
                     /gene="ilvC"
                     /locus_tag="ACHURJ_002689"
     CDS             complement(877..2352)
                     /gene="ilvC"
                     /locus_tag="ACHURJ_002689"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFR1281576.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRCHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            2502..3392
                     /gene="ilvY"
                     /locus_tag="ACHURJ_002690"
     CDS             2502..3392
                     /gene="ilvY"
                     /locus_tag="ACHURJ_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFR1281577.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSSEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(3389..4933)
                     /gene="ilvA"
                     /locus_tag="ACHURJ_002691"
     CDS             complement(3389..4933)
                     /gene="ilvA"
                     /locus_tag="ACHURJ_002691"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFR1281578.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRAVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(4936..6786)
                     /gene="ilvD"
                     /locus_tag="ACHURJ_002692"
     CDS             complement(4936..6786)
                     /gene="ilvD"
                     /locus_tag="ACHURJ_002692"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFR1281579.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDTLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(6851..7780)
                     /gene="ilvE"
                     /locus_tag="ACHURJ_002693"
     CDS             complement(6851..7780)
                     /gene="ilvE"
                     /locus_tag="ACHURJ_002693"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFR1281580.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(7800..8063)
                     /gene="ilvM"
                     /locus_tag="ACHURJ_002694"
     CDS             complement(7800..8063)
                     /gene="ilvM"
                     /locus_tag="ACHURJ_002694"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFR1281581.1"
                     /translation="MMQYQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(8060..9706)
                     /gene="ilvG"
                     /locus_tag="ACHURJ_002695"
     CDS             complement(8060..9706)
                     /gene="ilvG"
                     /locus_tag="ACHURJ_002695"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFR1281582.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPELQQPLNINDWQQYC
                     AQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(9709..9759)
                     /gene="ilvX"
                     /locus_tag="ACHURJ_002696"
     CDS             complement(9709..9759)
                     /gene="ilvX"
                     /locus_tag="ACHURJ_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFR1281583.1"
                     /translation="MNNSTKFCFSTFSTGN"
     gene            complement(9846..9944)
                     /gene="ilvL"
                     /locus_tag="ACHURJ_002697"
     CDS             complement(9846..9944)
                     /gene="ilvL"
                     /locus_tag="ACHURJ_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFR1281584.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            10296..11816
                     /locus_tag="ACHURJ_002698"
                     /pseudo
     CDS             10296..11816
                     /locus_tag="ACHURJ_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
     gene            complement(11841..12179)
                     /gene="maoP"
                     /locus_tag="ACHURJ_002699"
     CDS             complement(11841..12179)
                     /gene="maoP"
                     /locus_tag="ACHURJ_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFR1281585.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            12298..13137
                     /gene="hdfR"
                     /locus_tag="ACHURJ_002700"
     CDS             12298..13137
                     /gene="hdfR"
                     /locus_tag="ACHURJ_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFR1281586.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(13233..13308)
                     /locus_tag="ACHURJ_002701"
     tRNA            complement(13233..13308)
                     /locus_tag="ACHURJ_002701"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13273..13275),aa:Trp,seq:cca)
     gene            complement(13317..13393)
                     /locus_tag="ACHURJ_002702"
     tRNA            complement(13317..13393)
                     /locus_tag="ACHURJ_002702"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13357..13359),aa:Asp,seq:gtc)
BASE COUNT         3116 a         3638 c         3480 g         3198 t
ORIGIN      
        1 tgtcgtgctc aacggaaaaa ttaatggcaa cgcatgtatc gtcgttccat tatcaaccac
       61 tcgcgatcat gacaagttaa atcgtggaat gcatgttgaa atagccagta atgttatcaa
      121 tggcctgcaa ttctttgatc agcagattcg ttgggccaaa gccgacctgg tacaacaagt
      181 cagtcgaaac cgattaaata gagcaaggac ttacagaggg tatcttaatc aatgtttgcc
      241 acatgagttg gttgcagaca tacagagggc ggtcatcaag tcaatcaatg ccatattgct
      301 cataaattga caataaaata accatccccg ataatgactt tgtagcccat agaggcccta
      361 agttttctcc ccttgaggga cctgcgaagc ccggattttc cgggcttttg ccatatatgt
      421 accagcccct ccctgactga caattacgtt agcatctgcg gatcatgcta aaatcgccgc
      481 ctgacaatta ttcactcaag gcacgatcat ggcaaaaaca gcagcagcac tgcatatcct
      541 tgtaaaagaa gagaaactgg ctctggatct tctcgagcag attaagaacg gtgccgattt
      601 cggcaagctg gcgaagaaac actccatttg cccatcaggc aaacgcggcg gtgatttagg
      661 tgaattccgc cagggtcaga tggttccggc gttcgataaa gttgtgttct cttgtccggt
      721 actggagccg accggcccgc tgcacaccca gttcggttac cacatcatta aggtgctgta
      781 ccgtaactaa tagcaaggcc ttctccagga gaaggccttg agtgttttct ccctctccct
      841 gtgggagagg gtcggggtga gggcatcagc gcgcacttaa cccgcaactg caatacgttt
      901 catatccgtc atatagccgc gcagtttctt acctacctgc tcaatcgcat ggcagcgaat
      961 cgcttcgttc acatcacgca gttgcgcgtt atctaccgca ccttccggaa tagctttacc
     1021 cagatcgccc ggttgcagct ctgccataaa cggtttcagc aacggcacac aagcgtaaga
     1081 gaacagatag ttaccgtact cagcggtatc agagataacc acgttcattt cgtacagacg
     1141 cttacgggcg atggtgttgg caatcagcgg cagctcgtgc agtgattcat aatatgcaga
     1201 ctcttcaatg atgccggaat cgaccatggt ttcaaacgcc agctcaacgc ccgctttcac
     1261 catcgcgatc atcagtacgc ctttatcgaa gtactcctgc tcgccgattt tgccttcata
     1321 ctgcggcgcg gtttcgaagg cggttttgcc ggtctcttca cgccaggtca gcagtttctt
     1381 atcatcgttg gcccagtccg ccatcatacc ggaggagaat tcgccggaga tgatgtcgtc
     1441 catatgtttc tggaacaggg gtgccatgat ctctttcagc tgttcagaca gcgcgtaagc
     1501 acgcagtttc gccgggttag agagacggtc catcatcagg gtgatgccgc cctgcttcag
     1561 cgcttcggtg atggtttccc aaccgaactg aattagtttt tctgcgtatg ccggatcggt
     1621 gccctcttcc accagcttgt cgaagcacag cagcgagcca gcctgcaaca taccgcacag
     1681 gatggtttgc tcacccatca ggtcagattt cacttccgca acgaaggacg attccagcac
     1741 gcccgcacgg tgaccaccgg ttgctgccgc ccatgcttta gcgatcgcca tgccttcgcc
     1801 tttcggatcg ttttccgggt gaacggcaat cagcgtcggt acgccgaatc cacgtttgta
     1861 ctcttcacgc acttcggtgc cagggcattt cggcgcaacc atgactacgg tgatgtcttt
     1921 acggatctgc tcgcccactt cgacgatgtt gaaaccgtgc gagtaaccca gcgccgcgcc
     1981 gtctttcatc agtggctgta cggtgcgcac tacgtcggag tgctgcttgt ctggcgtcag
     2041 gttaaccacc aaatccgcct gcgggatcag ttcttcgtaa gtgcccactt taaaaccgtt
     2101 ttcggtcgct ttacgccagg atgcgcgctt ctcggcaatc gcttctttac gcagagcgta
     2161 ggagatatcg agaccagaat cacgcatgtt caggccctgg ttcagcccct gtgcgccaca
     2221 gccgacgata accacttttt taccctgaag gtagctcgcg ccgtcggcga attcgtcgcg
     2281 ccccataaag cgacatttgc ccagctgtgc cagctgctgg cgcaggttca gtgtattgaa
     2341 gtagttagcc atggtgattc ctcgtgatgt tgtgcttctt attgttcggt tcgcgtttgc
     2401 gagattgaat tcactatatg acaggaaatt tattgcggaa attgatatat tcacaacgtc
     2461 acattgcaat ttctgcaacg tcaacattga gggctgctcc attggattta cgcgatctga
     2521 aaaccttcct gcatctggcg gaaagccgcc attttggccg cagcgcgcgg gcgatgcacg
     2581 ttagcccatc cacgctctca cggcagattc agcgcctgga agaagatctt ggtcagccgc
     2641 tatttgtgcg cgataaccgc acggtgacgc tgactgaagc gggcgaagag ctgcgcgttt
     2701 tcgcccagca aacgctgttg cagtatcagc agttgcgcca caccatcgat cagcaagggc
     2761 cgtcgctctc tggcgaatta catatcttct gctcggtgac cgctgcctac agccatctgc
     2821 caccgattct cgaccgcttc cgcgcggaac acccgtcggt ggagatcaaa ctaaccactg
     2881 gtgatgcggc agatgcgatg gaaaaggtag tcactggcga agcggatctg gcgattgccg
     2941 ggaaaccgga gactctgccc ggcgcagtgg cgttttcgat gctggagaat ctggcagtag
     3001 tgctgattgc gcccgcgctg ccctgcccgg tgcgtaacca ggtttcgtca gagaagccgg
     3061 actggtctac agtgccgttt atcatggccg atcagggacc ggtacgccgc cgcattgaac
     3121 tgtggtttcg acgcaataaa atcagcaacc cgatgattta cgccacggtt ggcgggcatg
     3181 aagcgatggt atcgatggtg gcgctcggct gtggcgtggc gctgttgccg gaagtagtgc
     3241 tggaaaacag ccccgaaccg gtgcgtaacc gcgtgatgat tttagagcgc agcgatgaaa
     3301 aaacgccgtt tgaactgggc gtttgtgcgc agaaaaagcg gctgcatgag ccgctgattg
     3361 aggcgttctg gaagattttg ccgaaccatt aacccgccaa aaagaacctg aacgccggat
     3421 tattggtttc gtcgtggcaa tcgtagccca gctcatttag ccgggtttcg aaatccggtt
     3481 catggtcgcc gagttcgaac gccgccagta cgcgcccgta gtcggtgcca tggctgcgat
     3541 agtggaacaa agaaatgttc cagtgcgtac ccagcgtgtt gaggaagcgc agcagcgcgc
     3601 ccggtgattc cgggaattcg aagctgtaga ggcgttcctg caacggatgt gacggacgcc
     3661 cgccgaccat atagcgcaca tgcaacttcg ccatttcgtc gtcggagaga tccaccacgc
     3721 tgtagccgcc gtcgttgagc atctgcaaaa tttctttgcg ctcttcgagg ccgcggctca
     3781 ggcgcacgcc gacaaagatg caggcgtttt tggcatcggc aaaacggtag ttgaactcgg
     3841 tgaccgctcg cccgccaagc agttggcaga atttgaggaa gctgcctttt tcttccggaa
     3901 tggtcaccgc caacaacgct tcacgctgtt cgcccagttc gcagcgttcc gagacgtagc
     3961 gcaggccgtg gaagttcacg ttggcaccgg aaagaatatg cgccagccgt tcgccgcgaa
     4021 tgttgtgctg ggcgatatat tttttcattc ccgccagcgc cagcgcgcca gagggttccg
     4081 ccaccgcgcg cacatcttcg aataaatcct tcatcgccgc acagatcgca tcactatcga
     4141 cggtgatgat gtcgtcgaga tactcctggc ataaacggaa ggtttcgtca ccgatgcgtt
     4201 ttaccgctac gccttcagca aatagcccta cgcgcggcag atcaaccgga tgacccgcat
     4261 ccagcgctgc tttcaggcag gcggagtctt ccgcttctac ggcgatcact ttgatttgtg
     4321 gcatcagttg tttgatcagc accgcaacgc ccgccgccag accgccgccg ccgactggca
     4381 caaatacgcg gtcgagatgg gcgtcctgct ggagcagttc cagcgccagc gtgccttgcc
     4441 cggcaatcac catcggatgg tcgaacggcg gcacccaggt gaacccctgc tgctgtgaca
     4501 gttcgatcgc tttagctttc gcttcgtcaa agttcgcgcc atgaagcagc acttcgccgc
     4561 cgaatccacg taccgcatcg actttgatgt cggcggtggc ggttggcata acgatcaggg
     4621 ccttcacgcc taaccgcgca gaagaaaacg cgacgccctg cgcgtggtta cccgcagaag
     4681 cagtgatcac gccgtgcgct ttttgttctt ccgtcaggcc cgccatcatg gcgtatgcgc
     4741 cgcgcagctt aaagctgtgc actggctggc gatcttcgcg cttcaccaga atgacgttat
     4801 caagacgcga cgacagtttt tccatttttt gtagcggcgt aacctgcgct gcctcgtaaa
     4861 ccggcgcgcg cagcactgct cttaaatatt cggcaccttc cggagcacca gacaggggtt
     4921 gcgagtcagc cattattaac cccccagttt cgatttatcg cgcaccgcac ctttgtcggc
     4981 gctggttgcc aggctggcgt aggcgcgcag ggcaaaggaa acctgacgtt cacggttttt
     5041 cggcgtccag gctttgtcac cccgagcttc ctgcgcttca cgacgcgccg ccagttcggc
     5101 atcgcttacc tgtaactgaa tgccacggtt cgggatgtcg atagcgatca ggtcaccatc
     5161 ttcaatcaga ccaatgctgc cgccgcttgc cgcttccggt gagacgtggc cgatggaaag
     5221 accagaggtg ccgccagaga aacgaccgtc ggtgatcagc gcacaggctt taccgagacc
     5281 cattgatttc aggaagctgg ttgggtagag catttcctgc atgccagggc cgcctttcgg
     5341 gccttcatag cggatcacca ccacatcgcc cgcgacaact ttgccaccga ggatcgcttc
     5401 taccgcgtcg tcctggcttt cgtacacttt tgccgggccg gtgaatttga ggatgctgtc
     5461 atcgacgccc gcggttttca cgatgcagcc gttttcagca aaattaccgt agagcaccgc
     5521 caggccgccg tctttgctgt aggcgtgttc cagcgagcgg atacagccgt ttgcgcgatc
     5581 gtcgtccagc gtatcccaac ggcaatcttg cgagaatgcc tgggtggtac ggatacccgc
     5641 agggccggcg cggaacatat tttttaccgc gtcatcctgg gtcagcataa cgtcgtattg
     5701 ctccagcgtt tgcggcaacg tcaggccgag cacgtttttc acatcacggt tcagtaaccc
     5761 cgcgcgatcc agttcgccga gaataccgat aacaccacca gcacggtgaa catcttccat
     5821 atggtatttc tgggtgctcg gcgcaacttt acacagctgt ggaaccttgc gggaaagctt
     5881 atcgatatca ctcatggtga agtcgatttc cgcttcctgc gccgccgcca gcaggtgaag
     5941 tacggtgtta gtcgatccac ccatcgcgat atccagcgtc atggcgtttt caaacgccgc
     6001 cttactggcg atattacgcg gcagtgcact ttcgtcgttt tgctcgtaat aacgtttggt
     6061 caattcaaca atgcgtttac cagcattaag gaacagctgc ttacggtccg cgtgggttgc
     6121 cagcagcgag ccgttgcctg gctgcgacaa acccagcgct tcggtcaggc agttcattga
     6181 gttagcggta aacatcccgg agcaggaacc gcaggttggg caggcggaac gttcaacctg
     6241 atcgctctgg gagtcagaaa ctttcgggtc tgcgccctgg atcatcgcat caaccagatc
     6301 gagtttgatt atctgatcgg acagtttggt tttcccggcc tccatcgggc cgccggaaac
     6361 aaagatcacc ggaatattca ggcgcaggga agccatcagc atccccgggg tgattttgtc
     6421 gcagttagag atgcagacca tggcgtcggc gcagtgggcg ttgaccatat actcaacgga
     6481 atcagcgatc agttcgcgag atggaagtga ataaagcatc cccccgtggc ccatggcaat
     6541 cccatcatcc accgcaatgg tgttgaactc tttggcaacg ccgccagccg cttcaatttg
     6601 ttcggcgacc agtttaccga gatcgcgcag atggacgtga cccggtacaa attgggtgaa
     6661 cgagttcaca accgcgataa tcggcttgcc aaaatcggcg tcggtcattc cggtggcgcg
     6721 ccacagcgca cgagcacccg ccatattacg accatgagtg gtggtggcgg aacggtactt
     6781 aggcatactt tatttactcc cagtgtctgt ctcgtaaatg ggacggtgcg tgccgtccca
     6841 ttttttgtat ttattgatta acttgatcta accagcccca tttatcttcg gtttcgccag
     6901 tgaagaggcc gaagaaggct tgctgaatgc gtttggtaac cgggccacaa cggccttcgc
     6961 caacctgaat accgtctacg ctgcgcactg gcgtgatttc tgccgcagta ccggacataa
     7021 acacttcatc cgccaggtac agggattcgc gcgacagcac ctgctcacgc acttcaattc
     7081 ccagctcttt cgccagtttg atgatggcat cacgggtaat acccggcagc gcggatgagg
     7141 taaacggtgg ggtgaacagc acgccgtctt tcacttcaaa caggttttca cctgcgcctt
     7201 cagagatgta accgttcaca tccagcgcga taccttcctg ataaccgtgg cggcgcgctt
     7261 cgctacccac cagcagggaa gagaggtagt taccaccggc ttttgccgcc gtcgggatgg
     7321 tgtttggtgc tgcgcggttc caggaggaaa ccatcgcatc gatcccctgc tccagcgctt
     7381 ctgcgcccag atacgctccc cacgggaaag cagcgataat cacgtcggtt gagtatcccg
     7441 ctggcgggtt tactcccatg ccaacatcac cgacgaagat cagcggacgg atataggcgc
     7501 tggtgagatt atttttgcgg atcacgtcac gacaagcttc catcagctca tcaatgctct
     7561 gcgaaaccgg gaagcgatag attttggcgg agtcatgcag acgctgcata tgctcacgat
     7621 ggcggaatac aaccggtcct ttgtgcgagt cgtagcaacg gatgccttca aaaaccgaag
     7681 tgccatagtg cagcgcgtgc gacatcacat gcaccttcgc gtcttcccag cgaaccatct
     7741 ccccattgaa ccaaatgtaa tcagctttct tcgtggtcat ttttatattc cttttgcgct
     7801 caggcgcgga tttgttgtga tgtggttgtg ctctggcaga tggcaacgtg tgcgacgtcc
     7861 accagtttat ttaactgact aaacagtaag tcgaccgacc gtgggctggc aacggtcaat
     7921 tcgatattta tattttgtgc atcgctggcg gcggccatat tcattgagca gacgtggaaa
     7981 ccacgatgac gcaccacgcg taaaacacgt tctaaggttt ccggattgaa gcgagccgat
     8041 acattgacct gatattgcat catgataatt tctccaacat ttctgaatta ctggcgccag
     8101 gcggcaccag cggccagacg ttctcaagtt cgtcgattga gacatgaagc aggtatggcc
     8161 catcactgtt cagcatggtg tcgagtgccg cttcaacctg gtctttacgg gtgatgtgtt
     8221 ggccagggat gccgaaggcg ctggctaaca tgaggaaatc ggggttatca gtaagggtgg
     8281 tttcgctgta tcgttcctga aaaaacagtt gctgccattg tcgaaccatc cctaaccgtt
     8341 ggttatcgag taagacgatt ttcaacggta actgcttgcg ttttacggtg cccagctctt
     8401 gcacattcat catgaaagag ccgtcaccgg agatacagac gacggtatcg ttcggtcgcg
     8461 cgacttgtgc gccaaccgcc gctggtaaac caaaacccat agtgcctaag ccgctggagg
     8521 tgatgaaatt ttccgggcga gtgtgggcga tgtgttgcgc agcccacatc tggtgctgcc
     8581 ccacatctgt ggtcacgacg caatccgcag gtttacgatc cgacagttgt tttaacaaca
     8641 acggcgcgta gatagcgtcg ccgggatggt cgtaacgcca ggcatgttca tcacgcagct
     8701 gcgcgcagta ttgctgccag tcattgatat ttaacggctg ctgtaattct ggtaacagag
     8761 catttaaatc accttgtaat gccacatgtg cctgacgcag cttgttcatt tctgccgggt
     8821 cgatatccat atggataacg ctggcgtgtg gtgcgaaggt gttcagtttg ccggtcaccc
     8881 ggtcatcaaa acgtgcaccc acggcgatca gcaagtcgca ctcctgcacc gcgaagttcg
     8941 ccgctttggt gccatgcatt cccagcatgc ccagatagta cggataatct gcttcaactg
     9001 cgcccagccc tttcagcgtg caggtggcag gcatttttgt ggtagcgaga aattctcgta
     9061 aagcaggaac tgcctgcgcc atacccacgc caccaccaac gtacagcatc ggtttttgcg
     9121 cttttgccag catctggcgc gcttgctcaa cttcggcatg tgggaaagtc acttcgtttt
     9181 caacggtggt gaaccacggt tccaggtcac cgctggctag ctggatatct tttgggatat
     9241 cgaccagaac cggaccagga cgacctgagc tggcaacgtc gaatgcttca gccataatgc
     9301 gcggcaactc ttccagcgac tgcaccagaa agctgtgctt ggtacaggct aacgacaatc
     9361 ccaggacatc cacttcctga aatgcatcag tgccgataaa cggtgcggac acttgaccgg
     9421 tgatggcaac aacagggata gaatctaaca gtgcgtccgc aagcccggtt atcaggttgg
     9481 ttgcgcccgg accagacgtg gcgatacata cgccagtttt gccggtagca cgggcataac
     9541 cgatagccgc cattgccgca ccctgctcat gtcggcacag caagtgctcc acgccgccgt
     9601 catacaatgc atcgtaaacc ggcataattg cgccacccgg ataaccgaaa acggtgttca
     9661 caccctgtgc ccgcaacgca tgtaccaccc actgtgcgcc attcatagtt agttccccgt
     9721 gctgaatgtt gagaaacaga attttgtgct gttattcatt gtctgctcct cggttatgtt
     9781 tttaaggtca aaaaaaaccc ccggaccttt cggtgcgggg gtcttagttc gttaaggctt
     9841 gatttctaag cctttcctcg tccaagtgca gccccgcacg gtgggataat aatcaccacc
     9901 acgctaatca cgaccaggct aatcactcgt agaagggctg tcattttgtc ttttcttgca
     9961 tcttgttcga aggaatgcct aaagagttac cataggtttt gtaaatagta caagatattt
    10021 tttggccgat ttttatgatg cgtttaataa tttcaatata agttgttgtt ttttaaaaat
    10081 aaaatagtga gtaaaaaaat tcaatttcag tatgaatgtt gttcccttcc tcgtagttca
    10141 tcctttgtcc gctgcatttt tgcgagcatc attccaccgc cagaacaact caacttcact
    10201 tgcacaaata aacaggaatc agccactaaa attcacgaat tagcgggcta cagcagcgat
    10261 atcttatgca cataatggtg actcaaggag ggcttatgtc actgtcaatt gttcataccc
    10321 gcgcagccct gggagtaaat gcgcccccga tcactgttga ggtacatatc agtaaaggtc
    10381 tacccggctt aacgaaggtg ggcttaccag aaacaacggt aaaagaagct cgcgatcgcg
    10441 tgcgcagcgc cattatcaat agcggatatg aatatccggc gaaaaaaatc accatcaacc
    10501 tggctccagc tgatctgcca aaagaagggg gacgatatga tttacctatc gccattgcgt
    10561 tgctggcggc ctcagaacag cttacagcca ataagttaga tgaatatgga ttagtcggag
    10621 aactggcgct tacaggcgct ctgcgtggcg ttcccggcgc aatctccagt acaactgaag
    10681 ctattaagtc gggcagaaaa attatcgtcg cgaaagataa cgaagatgaa gtggggctaa
    10741 ttaacggtga aggatgcctg atagccgatc atctgcaagc tgtctgtgcg tttctggaag
    10801 gtaagcacgc tctcgaacgc ccgaaaccaa ctgatgcagt atcccgggcg ctacaacatg
    10861 atctcagtga tgttatcggt caggaacaag gaaagcgagg actggaaatt accgccgctg
    10921 gcgggcacaa ccttttactg attgggccgc cgggaacagg taaaacaatg ctcgccagcc
    10981 gtattaatgg cctgttgcca gatttaagca atgaagaggc actggagagt gctgcgatat
    11041 taagtctggt aaatgctgaa tcagtacaaa aacaatggcg gcagcgcccg ttccgctcac
    11101 ctcatcacag tgcatcgtta actgcgatgg taggcggtgg cgcaattcca gggcccggtg
    11161 aaatttcgct ggcgcataac ggcgtgcttt ttcttgatga gttacctgaa tttgaaaggc
    11221 gtacactgga tgccttgcga gagccgattg aatccgggca gatccatctt tcacgcacgc
    11281 gagcaaaaat aacctatcca gcccgtttcc agcttgtcgc ggcgatgaat cccagcccta
    11341 ccggacatta tcagggaaac cataaccgct gcacaccaga gcaaacgctg cgttatctca
    11401 accgactctc tggtcccttt ctcgaccgct tcgatctctc actggagatc ccattaccgc
    11461 ctcccggtat tttgagtaaa acggtagtgc cgggagaaag cagcgccacc gttaaacaac
    11521 gcgtaatggc cgccagagag cgccaattta agcggcagaa taaactgaac gcctggctgg
    11581 atagtccgga aatacgccaa ttctgcaaac ttgagagcga agatgcgatg tggctggaag
    11641 aaacgctgat ccatctgggg ttatcgattc gtgcctagca gcggttattg aaagttgcac
    11701 gaaccattgc tgatattgat cagtctgaca ttatcacacg tcaacatttg caggaggcag
    11761 ttagctatcg agcgattgac cgtttgctca tccatctgca gaaactactg acataaaaaa
    11821 agggcatttc gcccttttta ttaatcgtca gaatcggtgt agtcttcagc accttcaacc
    11881 tgcggtttac cgccggaaag ggtgtgaaaa cgttttggac gcttaatacg cgtcatatac
    11941 ttggaccaca cgcgttctgc ttctgtcact ggctcacgtt cgccacggca tactgctacg
    12001 aagagtttct cttcctcggt aaccggctcg cgtttgccaa gatccaactc attgaaggca
    12061 taaccatgac gctcaagcag ttgtgcctct ttgatggtga aatcaccatg acgagagaat
    12121 ccacgtggat aatgtttatt gtcgaaatat cgattagtcg tcgtaaagct ttccgccatc
    12181 ctgcacgctc ctaattcttt gaccgagcta gttatggcgc ggagtattag ttacgcttga
    12241 cagagtgtaa aacaaaacat ttaaatcata acgacaaata attttgcgga gagcactgtg
    12301 gatacggaat tgttaaaaac tttcctggaa gttagccgaa cgcgtcactt tggtcgagcg
    12361 gctgaatcgc tctatctgac ccagtcagca gtgagctttc gaatcagaca actggaaaat
    12421 caactgggtg tgaacctttt cacccgccac agaaacaata tccgtttaac cgctgccggt
    12481 gaaaaactac tgccttatgc agaaacgctc atgagcacct ggcaggccgc ccgcaaggag
    12541 gtggcgcata cctcacgaca taacgagttt tctatcggtg ccagcgcctc attgtgggaa
    12601 tgtatgctta atcagtggct gggacgtttg tatcaaaatc aggatgccca tacaggctta
    12661 cagttcgaag cgcgaattgc ccaacggcag tctctggtaa aacagctgca tgaacgccag
    12721 cttgatcttc ttattaccac tgaagcgccc aaaatggacg aatttagtag tcagttgctg
    12781 ggatatttca ctttagcgct ttataccagt gccccttcaa aactaaaggg agatcttaat
    12841 tatctgcgac ttgagtgggg gccagatttt caacagcatg aggcaggttt gatcggtgct
    12901 gacgaagtgc ccattctgac aaccagttct gctgaactgg cacagcaaca gattgcgatg
    12961 cttaatggtt gcacctggct acccgtcagc tgggcgcgta aaaaaggcgg cctgcatacc
    13021 gttgtcgata gcacaacact ttcacggccg ctttatgcca tatggctgca aaatagcgat
    13081 aaaaatgcgt tgattcgcga tcttttgaaa attaacgtgc tggatgaagt gtattaatat
    13141 gaatggctgg caaggatgcc ggtagaagga tttacttcgg agagggttat ttcagataaa
    13201 aaaaatcctt agctttcgct aaggatgatt tctggcaggg gcggagagac tcgaactccc
    13261 aacacccggt tttggagacc ggtgctctac caattgaact acgcccctaa ttagggtggc
    13321 ggaacggacg ggactcgaac ccgcgacccc ctgcgtgaca ggcaggtatt ctaaccgact
    13381 gaactaccgc tccaccgaat tcttttacaa ccaccggttt tatgaccggc tt
//
LOCUS       DAZPEB010000110        13406 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZPEB010000110 DAZPEB010000000
VERSION     DAZPEB010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13406
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.111"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..154
                     /locus_tag="ACHURJ_002703"
     CDS             <1..154
                     /locus_tag="ACHURJ_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2135 domain-containing protein"
                     /protein_id="HFR1281587.1"
                     /translation="NYYGGRSETELTTAQLTLITDEGSVNEKQETFIVPMRNAGELTL
                     VKSFDW"
     gene            429..3278
                     /gene="rcsC"
                     /locus_tag="ACHURJ_002704"
     CDS             429..3278
                     /gene="rcsC"
                     /locus_tag="ACHURJ_002704"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFR1281588.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGEPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(3478..4128)
                     /gene="rcsB"
                     /locus_tag="ACHURJ_002705"
     CDS             complement(3478..4128)
                     /gene="rcsB"
                     /locus_tag="ACHURJ_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFR1281589.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(4145..6817)
                     /gene="rcsD"
                     /locus_tag="ACHURJ_002706"
     CDS             complement(4145..6817)
                     /gene="rcsD"
                     /locus_tag="ACHURJ_002706"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFR1281590.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVNTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTISDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQPQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            7556..8686
                     /gene="ompC"
                     /locus_tag="ACHURJ_002707"
     CDS             7556..8686
                     /gene="ompC"
                     /locus_tag="ACHURJ_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFR1281591.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKSEDGDQTYVRLGFKGETQVTDQLTGYGQWEYQIQGNTSEDNKENSWTRVAFAGL
                     KFQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFG
                     LVDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGAAVSSS
                     KRTDDQNGSYISNGVVRNYIGTGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSL
                     GWANKAQNFEAVAQYQFDFGLRPSVAYLQSKGKNLGVINSRNYDDEDILKYVDVGATY
                     YFNKNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            8798..9853
                     /gene="apbE"
                     /locus_tag="ACHURJ_002708"
     CDS             8798..9853
                     /gene="apbE"
                     /locus_tag="ACHURJ_002708"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFR1281592.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MAEIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDEMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GINGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            9927..10979
                     /gene="ada"
                     /locus_tag="ACHURJ_002709"
     CDS             9927..10979
                     /gene="ada"
                     /locus_tag="ACHURJ_002709"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFR1281593.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCALGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVLANAIGKPKAVRAVASACAANKLAIVIPCHRVVGGDGTLSGYRWGV
                     SRKAQLLRREAENEER"
     gene            10979..11629
                     /gene="alkB"
                     /locus_tag="ACHURJ_002710"
     CDS             10979..11629
                     /gene="alkB"
                     /locus_tag="ACHURJ_002710"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFR1281594.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTIHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFYPLTTDCRYNLTFRQAGKKE"
     gene            11705..13348
                     /gene="yojI"
                     /locus_tag="ACHURJ_002711"
     CDS             11705..13348
                     /gene="yojI"
                     /locus_tag="ACHURJ_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFR1281595.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIEMA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLMAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         3253 a         3236 c         3494 g         3423 t
ORIGIN      
        1 caactactat ggtgggcgca gcgaaacgga attaaccacc gcccaactga cgctgatcac
       61 cgatgaagga tcggtcaatg agaaacagga aacatttatt gtaccgatgc gtaatgctgg
      121 cgagctgacg ctggtgaaaa gttttgactg gtgatccatc ccttcacaca acctggctct
      181 ctcttttttc agataagact gctgggcgat ctgccgacgc caggatataa ccattaacaa
      241 gcgtcggcag attggtcatt tcttattttt cgtactgaga actgtccatc tactccggct
      301 ttcctggtta aggcaatggt ttctaagcga tacgcattat tgcaataatg acagaacatt
      361 gcggtaacac gcttttaccg ctaccttaac cacactccat cggtcacctg aggcggagct
      421 tcgccccttt gaaatacctt gcttcttttc gtacaaccct gaaagcctcg cgctacatgt
      481 tcagagcatt ggcgttagtg ctctggctgt tgattgcttt ttcatccgtt ttttacatcg
      541 ttaatgcgtt acatcagcga gaatcggaaa ttcgtcagga atttaatctg agttccgatc
      601 aggctcagcg ctttattcaa cgcacctctg atgtgatgaa agagctgaag tacatcgccg
      661 aaaatcgctt atcggcagaa aacggtgtgc tttccccgcg tggacgagaa acgcaggcgg
      721 atgtgcctgc gtttgaaccg ctgtttgccg actccgattg ttccgcaatg agtaacacct
      781 ggcgaggttc tctggagtca ttggcgtggt ttatgcgcta ctggcgcgat aatttttctg
      841 cggcttacga tctaaaccgg gtatttttaa tcggcagcga taacctctgc atggccaatt
      901 tcggtctgcg tgatatgcca gtggaacgcg ataccgcgtt gaaagctttg catgaacgca
      961 tcaataaata tcgaaatgca ccacaagatg atagcggcag taacctctac tggatcagcg
     1021 aaggtccgcg ccctggcgtc gggtattttt acgcgttgac gccagtttat ctggcgaacc
     1081 ggttgcaggc gcttttgggg gtcgagcaga ccatccggat ggagaacttt ttcttaccgg
     1141 gtacgttgcc gatgggggtt accattcttg atgaaaatgg tcataccctg atttcgctta
     1201 ccggaccaga aagtaaaatt aagggcgagc ctcgctggat gcaggaacgc tcctggtttg
     1261 gctatacgga agggttccgg gagctggtgc tgaagaaaaa tctgccaccc tcatcgctaa
     1321 gcatcgtgta ttcggtgccg gttgataagg tgctggaacg cattcgcatg ttgatcctta
     1381 acgcaatttt gctgaatgtg cttgccggag ctgcattgtt tactctcgca cggatgtacg
     1441 agcgacgtat tttcattccg gcggaaagcg acgccctgcg actggaagaa catgagcagt
     1501 tcaatcgcaa gattgtcgcc tccgcgccag tgggtatctg cattttgcgt accgctgatg
     1561 gcgtcaatat tttaagtaac gaactggcgc atacctatct caatatgctt acgcatgagg
     1621 accgccaacg actgacgcaa attatctgtg ggcagcaggt caattttgtt gatgtcctga
     1681 ccagcaacaa taccaatctg caaatcagct tcgtccattc gcgctatcgt aatgaaaacg
     1741 tggccatttg tgtgctggtg gatgtttctt cgcgcgtgaa gatggaagag tcgttgcagg
     1801 agatggcaca agcagcggaa caggcgagcc agtcaaaatc gatgttcctt gccaccgtca
     1861 gtcatgagct gcgaacgccg ctgtatggca ttatcggtaa cctggatctg ttgcaaacca
     1921 aagagttacc gaaaggcgtc gatcggctgg tgacggcaat gaacaactct tccagcctgt
     1981 tgttgaaaat tatcagcgat attctcgatt tctcgaagat tgaatcggaa cagttgaaga
     2041 tcgaaccgcg tgagttttca ccgcgtgaag tgatgaacca catcaccgcc aactatttac
     2101 cgctggtggt acgcaagcag ttaggcttgt actgctttat tgaaccggat gtgccagtgg
     2161 ccttaaatgg cgacccgatg cgtttacagc aggtcatctc caacctgttg agtaacgcca
     2221 taaaattcac cgataccggc tgtatagttt tgcatgtccg cgctgatggc gattatctct
     2281 ctatccgtgt tcgcgatacc ggcgtgggga ttccggcgaa agaagtggtg cgcttgtttg
     2341 atcccttctt ccaggtcgga acgggcgtac agcgaaactt ccaggggacc ggtctgggtc
     2401 tggcgatttg tgaaaaactg atcagcatga tggacggcga tatctcggta gattcagaac
     2461 cgggaatggg cagccagttt accgtgcgta ttccgttgta cggcgctcag tacccgcaga
     2521 aaaaaggcgt ggaagggttg agtggtaaac gctgctggct ggcggtccgc aatgcgtcgc
     2581 tctgtcagtt cctggaaact agtttgcagc gcagcggcat cgtcgttaca acatacgaag
     2641 ggcaggaacc gactcccgaa gatgtgttaa ttactgacga ggtagtgagt aaaaaatggc
     2701 agggtagagc ggtagtgacc ttctgtcgtc gccatattgg tattccgctg gagaaagcgc
     2761 caggggagtg ggtacacagt gtggctgccc cgcatgagct accggcattg ttggcgcgta
     2821 tttatttgat cgagatggag agcgacgatc ctgctaacgc tctgccgtcg acggacaaag
     2881 cggtcagcga taatgacgat atgatgattc tggtcgtgga tgatcatccg attaaccgac
     2941 gtctgctggc agatcagttg ggatcgttgg gctatcaatg taaaaccgcg aatgatggtg
     3001 tcgatgcgct taatgtactt agcaagaatc atattgatat cgtgcttagc gacgtcaaca
     3061 tgccaaatat ggatggttac cgcttgacgc aacgcattcg tcagttggga ctgacgttgc
     3121 cggtaatcgg agtaactgct aatgcgttgg ctgaagagaa gcagcggtgt ctggagtccg
     3181 gtatggacag ctgcctgtcg aagccggtaa cgctggatgt gataaaacag acgctgacgg
     3241 tatatgccga gagggtcagg aaatcgcggg aatcgtagga cggataaggt gtttatgtcg
     3301 catctggcat tcagtgcaaa tgccagatgc gacgctgacg cgtcttatct ggcctacttt
     3361 gatgtaatct gttgaaataa tgggaatcgt aggacgcata aggcgtttac gccgcatccg
     3421 gcaatcggtg caaatgccag ataagacact aacgcgtctt atctggccta caggtgatta
     3481 gtctttatct gccggactta acgtcactga agagagataa ttcagcaggg cgatatcgtt
     3541 ctcgacaccc agcttcatca tcgcagattt cttctggcta ctgatggttt taatactgcg
     3601 gttcagcttt ttagcgatct cggtcaccaa gaagccttcc gcaaacaggc gcagaacttc
     3661 actctctttt ggcgagagac gcttgtcacc gtaaccacca gcactgattt tttccaacag
     3721 gcgagaaacg ctttccgggg taaatttctt ccctttctgc agcgcggcga gagctttcgg
     3781 cagatcggtc ggtgcacctt gtttcagcac gatcccttcg atatccagat ccaataccgc
     3841 actaagaatc gccgggttgt tgttcatagt cagaacaatg atcgacaggc ttgggaaatg
     3901 gcgcttgatg tacttgatta aggtaatgcc atcgccgtac ttatcgccag gcatggagag
     3961 atcggtaatc aacacatgcg catccagttt cggcaggttg ttgatcagtg ctgtagagtc
     4021 ttcaaattcg ccgacaacat tcacccactc aatttgctca agtgatttgc gaataccgaa
     4081 caagactatc ggatggtcat cggcaataat tacgttcata ttgttcatgt aataggctac
     4141 cttgctacag caagctcttg acataactgt caatgtcgct gatgtatttt tctattcctg
     4201 gaacatcctt ctcacgaatc agatgttcca gcgtttcaca taactgcttg ccgggtacca
     4261 gatttagcat ggcaaatacg cctttaagac gatgagccgt ttgggctaac gcagcaaagt
     4321 cactggttgc tgcttcagta tacagcctct taacatcatc cggtactgtg tctacaaaga
     4381 gcgcataata gccgctggca tggagttgcg cattttcatc tccgcccaga ggcgattctg
     4441 tcacctcttc ctgcgccagt tgcacttcaa ttaattgtaa gaccgcttcc tgcatagcgt
     4501 tgctcatatt gaagttgacg cacaattgac caggcccaat ttcccgtacg ccagactcat
     4561 catcgcttaa aagcaagcca gaggcagtaa gattagacgg attatccgtt aaaaagatat
     4621 cataatcttg actaattaat ctttcatcgg gtgtgataca ggttgcaccc caattttcta
     4681 actggcgagt gacaatattc cgaatttctg ccgaagtaac atccaccatt acgcagacat
     4741 catccagtaa acgctcttct tcctcttcaa cttccgggtc agcggcgagc attttgatat
     4801 gcacagagta gcgtgtacca agcccatccc gcgttttgat gtttaaatga ccgcccagtt
     4861 tacgcgccag ttgatcgctc agccagaatg ccagcgggtc cgccttgcca tagcgatcgt
     4921 tttggggctg gttgataaac gggaagtgca aattatccat ttcatgaata cttacgcctt
     4981 ctcccgtgtc cagaatgcgg aatgtcaggc ggtcttcaga ggactcatcc tgatcaacct
     5041 caagggtgat ttttcccaat tgcgttgagg tcacggcata ttgcatcagt agcagcaaaa
     5101 tacgtcgtaa ggcatcgcga tcgccgcggc gcatatcgtg tgctttcaga tgattgttaa
     5161 tcagcagttg cagaccttta cgcttgatgg caggcaacac tgaaggcaca acttcatcaa
     5221 ttaaatcctg cacggagaac agcaccgtct cacttttcca gctatcgtcc gcaagcatgt
     5281 tcgctaactg tatttcatcg accagccgca ccagcacatc tgcctgattc gccagttgtt
     5341 tgctttccgg ggcgttgagt ttagccgcgc tctccgccag ggactgtgcg ggttctttca
     5401 gcgcatcgcc aatgtttttc ataaaggtca tccgcccctg ctggtttttc tcatacagac
     5461 gctgcgcctg cttgagtttc ttgtttacca gcacttcgcg atcctgatcg cgaataatga
     5521 aaatttgtgt gcgcggcgcg acctggctgc ggaacatgcg gatctcatac agctcgttat
     5581 tgatcgtcgc ctgaataatc ccctgatgct gttccgccat ggtggtgatg ttttgcagat
     5641 tcaaatgcgg cagcaaatga tcggcaattt tgttacttat gacagtgcgg ttcgattcct
     5701 gatcgtgaac cagcaggccg agcggcagca gtgagactat ctcttcatta attgcccgta
     5761 aaatgcgcaa ttcgttattg accgccgtgc tgggtagact ttctgtactg cggctggaga
     5821 aatggcggaa tgtggtatag ccaaataacg ccagcgccag caaaccgatg ttcagcagca
     5881 gtggcagcag aatgttttgc aacgtatcca gcaataaggt gccataagga acctgccaga
     5941 ccaggcgcat atcggtagag ttgagtgccg aggagatttc aatcttcgta ctgttaaagt
     6001 ggatactgac gctatccgtc ccttctttct cattatcatt gtctcccgtt gccgtcgcgt
     6061 ctggctcaag gcggaaactg tccagcggca tacccggtgg gatcaaatca ttaatcggta
     6121 gatcaaaagc tacgaccgtt gccagatgtc ccggctggtt gaacgtggta cgcaaggtaa
     6181 agtaatgacc gttctgccag gccaggcggc gcatgttaga aaaactttcg cgttcatcga
     6241 gggcgttggc ctgttgcaac atctctgcac gacgtgaatc aacaatgtcg ctgatggtcg
     6301 attctttaaa tccggaggtg agatctttga ggggcagggt tgagatcagc accagactgt
     6361 tatcctgacc attcaggtaa tacatcgacc acggtacatt ttctgcgccc cacaatgtgt
     6421 ccagataggt ggacatccgc tgagtcatct caagcgttga gttgtcgtga gagccaaaga
     6481 ttaacgcttc cgttttgcgg cgcggtttct ccagatagta gacatcctgt ttcaggcgcg
     6541 tctcttgtaa accttcgccg gaggaggggg aggttgtcgc ggcaatgttg tcgtagatct
     6601 gccaggtcac gtaacgccag gtattgacgc gcttttggat agcgtgggta atgtcgacaa
     6661 tctggtaact tttatctttc agccatgcgt taacggcgct ttgtaccatt acacccatcg
     6721 tcaccagtaa cacaatgatc aacagtaaaa agaagcgggt aatgctcccc ggtaggagtg
     6781 aaaagcgggt cgtggccgtt gtctctttct gacgcattcg tgtttatgac ctgttaaaac
     6841 ttcgcgaagt gatagggcag tataaagggt acagtgtgaa attccagact cttacctgat
     6901 tgatgattct ctattctccc gtgatattta tcatcctgca ttgggggaaa tagatgtaca
     6961 agcgccattt tgctgtacaa ataacctaca aaaagcccaa cgaaattaat tgcaaataaa
     7021 cgatgattgc tggaaattat gctgatgtta attatttgtg aaatagttaa caagcgttat
     7081 agtttttctg tggtagcaca gaataatgaa aagtgtgtaa agaagggtaa aaaaaaccgg
     7141 atgcgaggca tccggttgaa ataggggtaa acagacattc agaaatgaat gacggtaata
     7201 aataaagtta atgatgatag cgggagttat tctagttgcg agtgaaggtt ttgttttgac
     7261 attcagtgct gtcaaatact taagaataag ttattgattt taaccttgaa ttattattgc
     7321 ttgatgttag gtgcttattt cgccattcca caataatctt aaaaagttcc cttgtattta
     7381 cattttgaaa catctatagc gataaatgaa acatcttaaa agttttagta tcatattcgt
     7441 gttggattat tctgcatttt tggggagaat ggacttgccg actgattaat gagggttaat
     7501 cagtatgcag tggcataaaa aagcaaataa aggcatataa cagagggtta ataacatgaa
     7561 agttaaagta ctgtccctcc tggtcccagc tctgctggta gcaggcgcag caaacgctgc
     7621 tgaagtttac aacaaagacg gcaacaaatt agatctgtac ggtaaagtag acggcttgca
     7681 ctatttctct gacaacaagt cagaagacgg cgaccagacc tatgtacgtc ttggtttcaa
     7741 aggcgaaact caggttactg accagctgac cggttacggc cagtgggaat atcagatcca
     7801 gggcaatacc tctgaagaca acaaagaaaa ctcctggacc cgtgtggcat tcgcaggtct
     7861 gaaattccag gatgtaggtt ctttcgacta cggtcgtaac tacggcgttg tttacgacgt
     7921 aacttcctgg accgacgtac tgccagaatt cggtggcgac acctacggtt ctgacaactt
     7981 catgcagcag cgtggtaacg gcttcgcgac ctaccgtaac accgacttct tcggtctggt
     8041 tgacggtctg aactttgctg ttcagtacca gggcaaaaac ggtagcgtaa gcggcgaagg
     8101 catgaccaac aacggtcgtg gcgctctgcg tcagaacggc gacggtgtcg gcggttctat
     8161 cacttatgat tacgaaggct tcggtatcgg tgctgcagtt tccagctcca aacgtactga
     8221 tgatcaaaat ggtagctaca tcagcaatgg tgtagttcgt aactacatcg gtactggcga
     8281 ccgtgctgaa acctacactg gtggtctgaa atacgacgct aacaacatct acctggctgc
     8341 tcagtacacc cagacctaca acgcaactcg cgtaggttcc ctgggttggg cgaacaaagc
     8401 acagaacttc gaagctgttg ctcagtacca gttcgacttc ggtctgcgtc cgtctgtagc
     8461 atacctgcag tctaaaggta aaaacctggg tgtcatcaat agtcgtaact acgacgacga
     8521 agatatcctg aaatatgttg atgttggcgc gacctactac ttcaacaaaa acatgtccac
     8581 ctatgttgac tacaaaatca acctgctgga cgacaaccag ttcactcgtg acgctggcat
     8641 caacactgat aacatcgtag ctctgggtct ggtttaccag ttctaatctc gattgatatc
     8701 gaacaaaggg cctgcgggcc cttttttcat tgttttcagc gtacaaactc agttttttgg
     8761 tgtactcttg cgaccgttcg catgaggata atcacgtatg gaaataagct ttacccgcgt
     8821 ggcactgctg gctgccgcgc tcttctttgt tggttgcgat caaaaaccac aacccgccaa
     8881 aacccacgct actgaagtta ccgttcttga aggcaaaacc atgggtacct tctggcgtgc
     8941 cagcatcccg ggcattgatg ccaaacgcag tgccgaactt aaagaaaaga ttcagaccca
     9001 gctggacgcc gacgatcagc tgctttcgac ctataaaaaa gattccgcgc tgatgcgctt
     9061 taacgactcg caaagtttgt cgccgtggcc agtaagtgaa gcgatggccg aaatcgtcac
     9121 tacctcgctg cgcattggcg cgaagaccga tggtgcgatg gatattaccg tcgggccgct
     9181 ggtaaatctg tggggctttg gcccggaaca acagccggtt caaattccga gccaggaaca
     9241 gatcgacgag atgaaagcca aaactggttt acagcacctt acggtcatta atcagtcgca
     9301 tcagcaatat ctgcaaaaag acctgccgga tttatatgtc gatctctcta ccgtcggcga
     9361 aggttatgcg gcggatcact tggcacgctt gatggagcag gaagggattt cccgctatct
     9421 ggtgtcggtg ggcggcgcgc tgaacagccg tggtatcaac ggtgaaggcc tgccgtggcg
     9481 ggtagcgatt caaaaaccaa ccgataaaga aaacgcagtt caggctgtgg tggatatcaa
     9541 cggtcatggg attagcacct ctggcagcta ccgtaactat tacgaactgg acggcaaacg
     9601 tctttcccat gttatcgatc cgcaaaccgg gcgtcccatc gaacacaatc tggtatccgt
     9661 gacggtgatt gctccgacgg cgctggaagc cgatgcctgg gatactggct tgatggtact
     9721 cgggccggag aaagccaaag aggttgttcg ccgggaaggg ctggcggttt atatgatcac
     9781 caaagaaggc gatagcttta aaacctggat gtcaccacag tttaaaagct tccttgtcag
     9841 cgaaaaaaat taaagcgcaa gattgttggt ttttgcgtga tggtgaccgg gcagactaaa
     9901 ggctatcctt aaccagggag ctgattatga aaaacgccac atgcttaact gacgatcaac
     9961 gctggcaatc tgtcttagcc cgcgacccga atgccgacgg cgaattcgtt ttcgccgtgc
    10021 gcaccacagg catcttttgc cgtccgtctt gccgcgccag acatgccttg cgggaaaatg
    10081 tctccttcta cgcaaatgcc agcgaggcac tcgccgccgg atttcgcccc tgcaaacgct
    10141 gccaacctga taaagccaat gcccagcaac atcggttgga taaaatcacc cacgcgtgtc
    10201 gactgctgga acaggaaacg cctgtaacgt tggaagcctt agccgaccag gtggcgatga
    10261 gtccatttca tctacatcgg ttgtttaaag cgactaccgg aatgacgcct aaagcctggc
    10321 aacaggcctg gcgcgctcgc cgtttgcgcg aatcgctggc gaaaggggag agcgtgacga
    10381 cgtctattct taacgccgga ttccccgaca gcagcagtta ctaccgcaaa gccgatgaaa
    10441 cgctgggcat gacggctaaa caattccgtc acggtggcga aaatctggcg gtgcgttacg
    10501 cgctggctga ttgtgcgctg ggtcgttgcc tggtggcaga aagcgagcgg gggatttgcg
    10561 cgatattgct gggcgatgat gacgcgacat taatcagcga gttgcagcag atgtttcccg
    10621 ctgccgacaa cgcgcctgcc gatctgacgt ttcagcaaca tgtgcgtgaa gtgatcgcca
    10681 gcctcaatca acgcgatacg ccgctgacgt taccgctgga tattcgcggc actgcttttc
    10741 agcaacaagt ctggcaggca ctgcgcacga taccttgcgg tgaaaccgta ctggctaacg
    10801 ccatcggcaa accgaaagcg gtacgggccg ttgccagcgc ctgtgccgcc aacaagctgg
    10861 ctatcgtaat accctgtcat cgagtggtcg gtggtgatgg cacactttcc ggttaccgct
    10921 ggggcgtgtc tcgtaaagcg caactgctgc gccgcgaagc tgaaaatgag gagaggtaat
    10981 gttggatctg tttgccgatg ctgaaccgtg gcaagagcca ctggcggctg gtgcggtaat
    11041 tttacggcgt tttgctttta acgctgcgga gcaactgatc cgcgatatta atgacgttgc
    11101 cagccagtcg ccgtttcgcc agatggtcac ccccggggga tataccatgt cggtggcgat
    11161 gaccaactgt gggcatctgg gctggacgat ccatcggcaa ggttatctct attcgcccat
    11221 tgatccgcaa acaaataaac cgtggcccgc catgccacaa agctttcatg atttatgtca
    11281 acgtgcggct acggcggcgg gctatccaga tttccagcca gatgcttgtc ttatcaaccg
    11341 ctacgctcct ggcgcgaaac tgtcgctgca tcaggataaa gacgaaccgg atctgcgcgc
    11401 gccaattgtt tctgtttctc tgggcttacc cgcgattttt caatttggtg gcctgaaacg
    11461 taatgatccg ctcaaacgtt tgttgttgga acatggcgat gtggtggtat ggggcggtga
    11521 atcgcggctg ttttatcacg gtattcaacc gttgaaagcg gggttttatc cactcaccac
    11581 cgactgccgc tacaacctga cattccgtca ggcaggtaaa aaagaataaa aataagaatt
    11641 attattgctg tgcgcgcgaa gatgtttaaa ctgcgggctg taattcattg tccgggtttt
    11701 ctgcatggaa cttcttgtac ttgtctggcg gcagtatcgc tggccattta tcagtgtgat
    11761 ggcgctaagc ctcgccagtg cggcattagg cattggctta attgctttta tcaatcagcg
    11821 ccttatcgaa atggcggata ccagtctgct ggtattgccg gagtttctgg gattattgct
    11881 gctgttgatg gcagtcactc tcggatcgca actggcgctc accactttgg ggcatcactt
    11941 cgtttaccga ctgcgtagtg aatttatcaa gcggattctg gatacccacg tcgagcgcat
    12001 tgaacaactc ggtagcgcct cgttgttggc gggattaacc agcgatgtgc gcaatatcac
    12061 cattgctttt gtgcgtcttc cggaactggt gcaggggatc attctcacta tcggttccgc
    12121 ggcgtatctg tggatgctgt cgggcaaaat gttgctggta acggctatct ggatggcgat
    12181 caccatctgg ggtggttttg tactggtggc gcgggtgtac aaacatatgg cgaccctacg
    12241 tgaaaccgaa gacaagctgt acacggattt tcaaactgta cttgaagggc gcaaagagct
    12301 gactctgaac cgggaacgcg ccgagtatgt gtttaacaac ctctacatcc ccgatgcgca
    12361 agagtatcgc catcatatta ttcgcgcaga caccttccat cttagtgccg tgaactggtc
    12421 aaacatcatg atgctgggcg ctatcggcct ggtgttctgg atggcgaaca gtctcggttg
    12481 ggctgatacc aacgttgctg cgacctattc gttgacgctt ttattcctgc gtacgccgct
    12541 gctttcggcg gttggcgcat tgccgacgct gctgatggcg caggtggcgt ttaacaagct
    12601 gaacaaattc gcgctcgcgc ctttcaaagc agagtttccg cgcccgcagg cgtttcccaa
    12661 ctggcaaacg ctggagctgc gtaatgtgac gtttagctat caggataacg cgttttccgt
    12721 tggtccgatt aatctcacca tcaaacgtgg cgagctactg tttctgattg gtggtaacgg
    12781 tagtggaaaa tcgacgctgg cgatgttgtt gacgggcttg tatcagccac aaagcggcga
    12841 aatcttgctg gatggcaaac ctgtcagcgg cgaacaaccg gaagattatc gcaaactgtt
    12901 ttcggcagtg tttaccgatg tctggctttt tgatcaactg ctggggccgg agggtaaacc
    12961 cgctaacccg caactggttg agaagtggct ggcgcagctg aaaatggctc ataagcttga
    13021 gttaagcaac gggcgtattg ttaacctgaa gttatcaaaa gggcagaaaa aacgcgtggc
    13081 gctgttgctg gcgctggcag aagaacgcga tattatcctg ctggatgaat gggcggcgga
    13141 tcaggatcca cacttccgtc gtgagtttta tcaggtgttg ctgccgctga tgcaggagat
    13201 gggtaaaact attttcgcta tcagtcatga tgatcattac tttatccacg ccgaccgcct
    13261 gctggaaatg cgcaatgggc aacttagcga gctgacgggc gaagagcgcg atgccgcttc
    13321 gcgtgatgcc gttgcccgga cggcataata ttacgacgct gacctggcgg actgctgccg
    13381 tcaggtcaat atgctaaaaa tccctt
//
LOCUS       DAZPEB010000111        13368 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZPEB010000111 DAZPEB010000000
VERSION     DAZPEB010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13368
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.112"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(172..1095)
                     /gene="dmlR"
                     /locus_tag="ACHURJ_002712"
     CDS             complement(172..1095)
                     /gene="dmlR"
                     /locus_tag="ACHURJ_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HFR1281596.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            1198..2283
                     /gene="dmlA"
                     /locus_tag="ACHURJ_002713"
     CDS             1198..2283
                     /gene="dmlA"
                     /locus_tag="ACHURJ_002713"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HFR1281597.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFDLSFEQMG
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YINLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTYATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            2533..4143
                     /gene="yeaV"
                     /locus_tag="ACHURJ_002714"
     CDS             2533..4143
                     /gene="yeaV"
                     /locus_tag="ACHURJ_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HFR1281598.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVIPAGTVFKGDN"
     gene            4175..5299
                     /gene="yeaW"
                     /locus_tag="ACHURJ_002715"
     CDS             4175..5299
                     /gene="yeaW"
                     /locus_tag="ACHURJ_002715"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HFR1281599.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNMITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTSANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            5355..6320
                     /gene="yeaX"
                     /locus_tag="ACHURJ_002716"
     CDS             5355..6320
                     /gene="yeaX"
                     /locus_tag="ACHURJ_002716"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFR1281600.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHVI
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGNRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(6374..7489)
                     /gene="rnd"
                     /locus_tag="ACHURJ_002717"
     CDS             complement(6374..7489)
                     /gene="rnd"
                     /locus_tag="ACHURJ_002717"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFR1281601.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     SAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLTLVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(7570..9255)
                     /gene="fadD"
                     /locus_tag="ACHURJ_002718"
     CDS             complement(7570..9255)
                     /gene="fadD"
                     /locus_tag="ACHURJ_002718"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFR1281602.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGVGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAVVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(9460..10041)
                     /gene="yeaY"
                     /locus_tag="ACHURJ_002719"
     CDS             complement(9460..10041)
                     /gene="yeaY"
                     /locus_tag="ACHURJ_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFR1281603.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGRSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(10081..10776)
                     /gene="tsaB"
                     /locus_tag="ACHURJ_002720"
     CDS             complement(10081..10776)
                     /gene="tsaB"
                     /locus_tag="ACHURJ_002720"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFR1281604.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPATEDMLPIACQIFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(10834..12744)
                     /locus_tag="ACHURJ_002721"
     CDS             complement(10834..12744)
                     /locus_tag="ACHURJ_002721"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFR1281605.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            12876..13220
                     /locus_tag="ACHURJ_002722"
     CDS             12876..13220
                     /locus_tag="ACHURJ_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFR1281606.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         3201 a         3555 c         3333 g         3279 t
ORIGIN      
        1 aaccagatag gtccagtaat tcagaacccc gtattcagcg aacacattaa cctctttaat
       61 tatctttcga tcatgcgcga ttaaaggtga atatgctaac caatctgtag cggcttagaa
      121 aggagaaaat caggttttaa cccgatatca acccgataat tgaatcatta atcaggcatg
      181 ctccagtgaa aaattcgggt agtgctcggc aaaatactgg cgtaaaaatt ctaccgttat
      241 ccgcactttc gctgacgtcg ccagccttga aacataaacg gcccagacgt tcgctggctg
      301 gtaatattcc ggtagcactt gcactaaatg accactggca atgttttcgc taacatccca
      361 ccaggaacgc agagcaatcc cctgcccatc gagacaccac tgatggacaa tttctccatg
      421 attagacgac agtgggccgg tgactttaat cgcatggtgg ccttctttat tgcgtaattg
      481 ccagacacca aaaggatgat cgcgctcttt aatgaccagg cacggcaatg cagaaagatc
      541 ggttaaatgt ttcggcgcgc catgttgcgc aataaattcc ggcgacgcgc agagaatgcg
      601 gtaattggtc gccagtttgc gggcgattaa attaggggcg atgtcatcgc caatgcgaat
      661 atcgagatcg acaccttcat tgaccaaatc gaccagccgg tcttccacat caaaacgtaa
      721 ttccagttgc ggatacgcct tcgccagcgc tgataacgcc ggagccacca cctgtcgtcc
      781 aaaaccaaag ctgctgataa tacgcagcat cccctgtggc acctgacgca cgtcagaaag
      841 ttcgtccatc atctgaccga catcctgcaa aatccgctgc gcccattcat aaatccgctc
      901 tccttcttcg gtaatggtga cgcggcgggt ggtgcggtgt aacaacacca cgtttagcgt
      961 ttgctccagc aaggcgatgc gcttgctgac gaacgccggt gaaacgccca gttcttccgc
     1021 cacggcggca aaaccagccc gacgagccac cagcataaag acgcgcaaat cattcagcag
     1081 cggtaaatta ttcatgattc gtgttttatg tttcaccagt tacggggatt aattcctttt
     1141 ccgtcaatta taggatggtg atgttgtcaa ttttgatggt caggaagtga gaacccaatg
     1201 atgaaaacga tgcgtattgc tgcgatcccg ggagacggga ttggtaaaga agtccttcct
     1261 gaagggattc gcgtgttaca ggctgccgct gagcgctggg gcttcgactt gagttttgag
     1321 caaatggggt gggcgagctg cgagtattac agccatcacg gtaaaatgat gccggacgac
     1381 tggcatgagc aacttagccg tttcgacgcc atctattttg gtgccgtcgg ctggccggat
     1441 accgttccgg accatatttc gttgtggggt tcgctgctga aatttcgtcg tgaattcgac
     1501 cagtacatca acctgcgccc ggttcgtctc tttcctggcg ttccctgccc gctggcggga
     1561 aaacagcctg gcgacatcga tttttacgtg gtcagggaaa acaccgaagg cgaatattcc
     1621 tcgctcggcg gtagagtgaa tgaaggtaca gagcatgaag tcgtcattca ggaatcggta
     1681 tttacacgtc gtggtgtcga tcgcattttg cgttatgcct tcgaacttgc gcaaagccgc
     1741 ccacgtaaga cgctaactta tgccactaaa tcgaacggtt tagccatcag catgccgtac
     1801 tgggatgagc gagtggaagc aatggccgag aattacccgg agatccgctg ggacaagcag
     1861 catattgata ttctctgcgc gcgttttgtg atgcagccgg aacgcttcga tgtggtggtg
     1921 gcgtccaatt tgtttggcga tatcctttcc gatcttggcc cggcctgcac cggcaccatt
     1981 ggcattgccc catccgccaa cctgaatccg gaacgcactt tcccgtcgct cttcgagcct
     2041 gtccacggtt ccgcgccgga tatctacggg aaaaatattg ctaaccctat cgccacgatt
     2101 tgggccgggg caatgatgct cgattttctc ggcaatggcg atgagcgttt ccagcaagcg
     2161 cataacggta ttctggcagc aattgaagaa gtgattgctc acgggccgaa aacacctgat
     2221 atgaaaggca atgccaccac gccacaggtt gccgacgcga tttgcaaaat tattttgcgt
     2281 taaggtcaaa ccagtttatt tgaaccgcgt cactgacgcg gttttttatt cgttctttgc
     2341 agtaaataac ctgcgtcatt tcacctttta ttgtttccgt ttcgtgtttt atggctttcc
     2401 gcattcttaa ttgtttaatt tatgtaatat tcaaataatc aatttgttaa acacaacata
     2461 caactcatta ccctgcaaat aattacaact ggcaaaagcc agagacgggc ttttatgccc
     2521 aggagatgac tcatgatgag caatgttaag aaaaaagatg tgccgctgat aagcatcagc
     2581 ctggtggcca ttcttttcat cgcagctgca ttaagccttt tcccacaaca atcggccgac
     2641 gcggccaacg caatatacac ttttgttacg cgtacgttag gttccgccgt acaggtattg
     2701 gttttgctgg caatgggact ggtgatttat ttagccacca gtaaatacgg caatattcgt
     2761 cttggcgaag gaaaaccgga atacagcacg ctctcctggc tgtttatgtt tatttgtgcc
     2821 ggtttaggtt cttctacgct ttattggggg gttgctgaat gggcctatta ttatcaaaca
     2881 cctggattaa atatcgcacc gcgttcacaa caggcactcg aatttagcgt tccctactct
     2941 ttcttccact ggggcatcag cgcctgggca acttatacgc tggcctcatt aatcatggct
     3001 tatcactttc atgtgcggaa aaacaaaggt ctgagccttt ccggcattat tgctgctatt
     3061 accggcgttc gcccgcaagg cccatgggga aaactggttg atttgatgtt cctgatcgcc
     3121 actgtcggcg cactgactat ttcccttgtt gtcaccgcag caacctttac tcgtggactt
     3181 tccgcactga caggtttacc cgacaacttc accgtgcagg catttgtgat cctgctttct
     3241 ggcggcattt tttgcctaag ctcgtggatt ggtatcaaca acggtttgca acgtctgagc
     3301 aaaatggttg gctggggcgc gttcctgctg ccattactgg tgctgattgt cggcccaacc
     3361 gaatttatta ccaacagcat catcaatgcc atcggcctga ccacgcaaaa cttcctgcaa
     3421 atgagcttat tcaccgatcc gcttggcgat ggttcattta cccgcaactg gaccgttttc
     3481 tactggctgt ggtggatctc atacacccct ggcgtagcaa tgtttgtcac ccgcgtttcc
     3541 cgcggtcgta agattaaaga agttatctgg ggactgatcc tcggcagcac cgtcggttgc
     3601 tggttcttct tcggcgtaat ggaaagctat gccattcatc agtttatcaa tggcgtaatc
     3661 aacgtcccac aggtgctgga aacactgggc ggcgaaacag ctgtacagca agttctgatg
     3721 tcgttgccag ccggtaaatt gttcctcgcc gcatacctgg gcgtgatgat tattttcctt
     3781 gcctcgcata tggatgcagt ggcctacacc atggcggcga ccagtacgcg taatctccag
     3841 gaaggtgacg atcctgaccg tgggctgcgt cttttctggt gcgtggtgat cactctgatc
     3901 ccgctttcca tcttgtttac cggtgcttcg ctggaaacga tgaaaaccac cgtcgtgctc
     3961 acagcccttc ccttcctcgt cattttactg gtgaaagtcg gcggatttat tcgctggctg
     4021 aaacaggatt acgccgacat tccggctcat caagttgaac attatctccc gcagacaccg
     4081 gttgaagccc tggaaaaaac accagtgatc cctgcgggaa ccgtattcaa aggcgacaac
     4141 tgagcgccat catcctaacg ataaaggtat ccctatgagc aatctgagcc ctgactttgt
     4201 actacccgaa aatttttgcg ctaacccgca agaggcgtgg accattcctg cccgttttta
     4261 taccgatcag aacgcgtttg aacacgaaaa agagaacgtc ttcgccaaaa gctggatttg
     4321 cgtcgctcac agcagcgaac tggcgaatgc caatgattat gtgacgcgtg agatcattgg
     4381 cgaaagcatc gtgctggtac gcggtcgtga taaggttttg cgcgcgttct ataacgtgtg
     4441 tccgcaccgt ggtcatcagt tgttgagcgg tgaaggaaaa gcaaaaaata tgattacctg
     4501 cccgtatcac gcatgggcat tcaaactcga tggcaatctg gcccatgcac gtaactgcga
     4561 aaacgtcgcc aatttcgata gcgacaaagc gcaactggtt ccggtgcgtc tggaagaata
     4621 tgccggattc gtcttcatca acatggaccc caacgccacc agcgtagaag atcagttacc
     4681 cggtctgggc gcgaaagtgc tggaagcctg cccggaagtc cacgatctga aactggcggc
     4741 ccgctttacc acccgcacgt ctgccaactg gaagaacatt gtcgataact atctcgagtg
     4801 ctatcactgt ggtccggcgc atccaggttt ctccgactcc gtacaggttg atcgttactg
     4861 gcacaccatg cacggtaact ggacgctgca atacggtttc gccaaaccgt ccgaacagtc
     4921 gtttaaattt gaagagggta cggatgcggc attccacggt ttctggctgt ggccgtgcac
     4981 gatgctgaac gtcaccccga tcaaagggat gatgacggtc atttatgaat tcccggtgga
     5041 ttctgaaact accctgcaaa actacgatat ttacttcacc aatgaagagt taaccgacga
     5101 gcaaaaatcg ctgattgagt ggtatcgcga tgtgttccgt ccggaagatt tacgtctggt
     5161 tgaaagcgta cagaaagggc tgaaatcgcg tggctatcgt ggtcaggggc gcatcatggc
     5221 cgacagtagc ggtagtggca tttccgaaca tggtatcgcc catttccata atctgctggc
     5281 gcaggtgttt aaggactaat gacatcggcg gcggtatttt ccgctgctgg gctgattttt
     5341 gatggagtac agcaatgtca gactatcaaa tgtttgaagt acaggtgagc caggttgaac
     5401 cccttaccga acaggtgaaa cgcttcacgc tggtggcaac cgatggcaaa ccgctgcctg
     5461 cgttcaccgg aggaagtcac gtcattgtgc agatgagcga tggtgataac cagtacagca
     5521 atgcgtattc actactgagt tcgccgcatg acacctcttg ttatcagatt gccgttcggc
     5581 tggaggaaaa ctcgcgcggc ggttcccgct ttttgcatca gcaggtaaaa gtgggcaatc
     5641 ggttaacgat ttcaacgcct aataacctgt ttgcgctaat tccctcagcc agaaagcatc
     5701 tgtttatcgc gggcggtatt ggtatcaccc ctttcctgtc gcacatggca gagctgcaac
     5761 acagcgacgt cgactggcag ctacattact gctcgcgaaa tccagaaagt tgcgcatttc
     5821 gtgatgagct agtccagcat ccgcaggctg agaaagttca cttgcatcac tcatcaaccg
     5881 gaacacgact ggaattagcg cgattattgg cggatatcga acctggcaca cacgtttata
     5941 cctgtggtcc cgaggcgcta aatgaagcgg taagaagtga agctgcgcgt ctggacatcg
     6001 ccgccgatac gctgcacttt gagcaatttg ctatcgaaga caaaaccggc gatgccttta
     6061 ccctggtgct tgcccgttcc ggaaaagagt ttgtggtgcc ggaagagatg actattttgc
     6121 aggttattga aaataataaa gccgcgaaag tggaatgttt atgtcgtgaa ggggtatgcg
     6181 gaacctgcga aacagcaata ctggaaggtg aagctgacca tcgggatcag tattttagcg
     6241 atgaagagcg tgccagccag caaagtatgt tgatctgttg ttcgcgtgcg aagggtaaac
     6301 gcctggtgtt ggatttgtag tttgccaaag ccggatgtgg cgctgagcgc gcccagtccg
     6361 gcttcggaag attttactgc ggatattcct gcaataaatt gtgtaatgct tccgccatca
     6421 gctcaccacg ccagccggaa atcagctccg gcaaattgtt ctgcggtttc agtttccagt
     6481 gccagttcag cagttggttg atttgccgac gcgatgccag caattcggcg ctgatcttat
     6541 gcgtttcgct cacgtcagta atcagcgact taatcgcttt aaacgcttta cgataaccag
     6601 gcatgtccat caggttaagc atcggctgcg gtaaggcctc ttccggcaat gtctgcgctt
     6661 tttccaccag cgtcagcagc gttttaccgt gaaagcggat ttcgctaccg gataaaccca
     6721 ggctgtccag ttcgcctaaa cttcccggca tataacgcgc gaccgaccac aaatgctctt
     6781 cacgcacgac aaagttcacc gccagatcac gctctcgcgc cttgcgcagt cgccagtcgg
     6841 ctaacagttg cagacaggcc agttggcgtg tgcgtaactg ccaggcattg gtgatatcac
     6901 gccaggcatc ttccggcgca acgacttcct gacgacgcat ttgcatcagg cggcattcat
     6961 ccagcgccgc aggtagccag ccggaggcct ccgtttctac cataagcttg gcggtgatcg
     7021 gtaacagata ccagacatcc gccgctgcgt attcacactg acgttcggtc agcggtctgg
     7081 ccagccagtc ggtgcgcgat tcactcttgt ccagcgtaac gccggaatac tcttccacca
     7141 tggaagcgaa accccatgac atcgggcgtc cgcagaaggc agaaaggatt tgcgtgtcaa
     7201 tcaagggttg tggtaattcg ccaaagacat tgaggaacac ttccagatct tcactgcctg
     7261 catggagaaa ttttgtgatg gacggatcgc gcaggatcgc tttcagcggt gaccagtcgg
     7321 tgatcccgag aggatcaatt agcgccagat gctcgccatc gaaaagttga atcaacccca
     7381 gctgcgggta ataagtgcgc gtacgaacaa attcagtatc cagggctatc gccggaaagg
     7441 cacggacggc ttcacacaaa gaagccagcg catcgtccgt ggtaatcatt tggtaattca
     7501 aattgttttc tctttagtgg gcgtcaaaaa aacgccggat taaccggcgt ctgacgactg
     7561 acttaacgct caggctttat tgtccacttt gccgcgcgct tcgtcacgta attctcgtcg
     7621 caaaattttt ccgacgttag atttcggtaa ctcatcacga aactccacca gcttcggtac
     7681 tttgtatccc gtgagctgac ggcggcaaaa agtcaccagt gactcttcgg taagcgatgg
     7741 atcttttttc actacgaaga ttttcaccgc ttcaccactg gagccggaag gtacgccaac
     7801 agccgcgact tcctgtacgc caggatgctg catgacgaca tcttcaatct cgttgggata
     7861 gacgttaaaa ccggaaacca gaatcatgtc ttttttacga tcgacaatgc gcaggaatcc
     7921 ttcttcatcc attaccgcga tgtcgccggt gtgtaaccag ccatttttga tgatttcatc
     7981 ggtagcatcg ggacgctgcc agtaacccag catcacctgc ggtcctttga cacaaagctc
     8041 acccggttga cctggtggta cttcattatc atcatcatcc accagtttgg cttccgtcga
     8101 cggcaccggc aaaccgatgc taccactatg ataatcaata tcatatgggt taacgctgac
     8161 cagcggcgca cactcggtaa ggccatagcc ttccagcaga tactgtccgg tcagtttcac
     8221 ccaacgctct gccaccactt gctgcactgg catcccaccg cctgcggaaa gatgcagact
     8281 ggagaaatcc agctgctgga actctttatt gttcagcaac gcattgaaca aggtgttaac
     8341 gccagtgata gcggtaaacg gatatttcgc taactctttt accaaccctg gaatatcacg
     8401 cgggttagtg ataagcaggt tctgcccacc cagttcgata aacagcaggc agttaatggt
     8461 cagggcaaaa atgtgataca gcggcagtgc cgttactacc agttctttac ctggatgcag
     8521 caacggccca taggtggcgt tgacctgttc caggttcgcc agcatattgc ggtgagtcag
     8581 catcgcgcct ttcgccacac ctgtggtgcc gccggtgtat tgcaggaagg ctaaatcttc
     8641 cggcaccagt tcgggtttga cgtactgcat ccggtagccg ttatgcagtg cgctacgaaa
     8701 tgaaatggca tctggcagat ggtatttcgg caccaaacgc ttgatgtatt taacaacgaa
     8761 attgactacc gtgccttttg ccgtagatag ctgatcgccc atacgggtca gaattacgtg
     8821 ctgaacggcg gttttatcaa ccactttttc cagtgtgtga gcaaagttag acacgataac
     8881 aaccgccgat gcgccgctat cgttaagctg atgctcaagc tcacgcgggg tatacaacgg
     8941 gttaacgttt acgacgatca tcccggcacg caaaatgcca aacagcgcca ccggatattg
     9001 caataaatta ggcatcatca acgcaacgcg atcgcctttc ttcagcccca ccccttgttg
     9061 caaataagcg gcaaacgcgc gactgcgttc ttccagcttg cggaaggtca tcacctcccc
     9121 catattcaca aacgcaggct gatcggcgta gcgcgcgacc gactgctcaa acatatctac
     9181 cagagattga taacggtcag ggttgatctc cgtcggaacg tccgcgggat aacggttaag
     9241 ccaaaccttc ttcaattctt cacctctaaa atgcgtgttc gtcgtcatcg caaccccaaa
     9301 tgatatacat gccgttaaca taatattaac tcatcatacc agcttgataa ttacccaacg
     9361 aaaaggttgc gaagcgcgtc actatttatt tttatcttta ccgtaagaat gcagaaacag
     9421 cggaccagcc gctgtttctt tttctttaaa aacaagcaat tattcagtta caactgtttg
     9481 tactctcgcg ggaccgggat tataccagcc ccatccgccg tagccatagg gccagccacg
     9541 accgccataa aaccatggat caatcggctg aggcggcata atcacctgct gggttaaatg
     9601 ccaacgtttg taacccgtta cttgcatcac cataaattta tagggcgtat tgccgatttt
     9661 gccgtcaacc gcaccagtga ttggcccgac taccgtaacc agttgtccac ggaaatccac
     9721 cgggtccaga aaaccgttca catcggcata aatgcgaccg cgagaaggtt ctcccagcgt
     9781 cggtctggct ccgctgtcca gcggtacggt agcaatttcc aggcgggttt tcccttgctg
     9841 gttttgtacc gcaaccactt tgccaccaaa gcgtgcctcc tgaccaacgt acagctgcgg
     9901 cgcactcatc acccgaacta aatcttgttg cggcgtggga ctgcggcctt taatggcgtc
     9961 cggcacagtg acacaaccgc tcagcattag cgcaaacgta cctgccagta tgcctttgat
    10021 aacattcttt tgaaccgcca tgatgcgact cctttttttc tcagagcata ctcttaagat
    10081 tcattctttg cccggaagtt tcttccatgc gacgttgtta cgtaaataaa ccggttcggc
    10141 atgttccacc gccaccgttt taccctcggc aaatatctga cacgcaatcg gcagcatatc
    10201 ttccgtcgct ggcagtaaaa cttctccgtc gcgcaaaacc agcccgctct ctttaccgag
    10261 atccggccag gcttgccagc ccgtccctac cgttacccat tcgccggaaa gctgttgcat
    10321 tcgttcatga acgagttcgg gtttgagtac ggcttcggtt tcttcaccgc gccagatacc
    10381 gttttcatca cgctgatatt cggcccagta aacttcgccc atccgcgcat caatggcagc
    10441 cagcacgcgg gttgcgccgt ttttgcgcca cgccccttgc gccattgtca ttagcgtgga
    10501 gacgccaatc atcggcagct ctgcgccaag tgccagccct tgcgcaatgc caataccaat
    10561 gcgcacgccg gtaaagctac cggggccgcg cccgtaagcc agagcgttaa tatcagtcag
    10621 ggaagttccg ctggtggtca ggatatcctg caccatcggt aagattcgtt gagtatgttc
    10681 acgagggcaa agctcaaaat gagcgttgac agtaccgtcg ttccacaggg cgacagagca
    10741 ggcctctgtc gcggtatcga tagccagaat tcgcatgggt cttcgtgctt agatcaataa
    10801 aaaggcgcgc atcataccat actccgtaac aaattacctg gaggatggta tcgcaaggaa
    10861 acgaaccgca cgggcaatgt cacgggtgcg cggcgcgggc ggcagactgg cgagaaacgt
    10921 cgcgccgtaa gggcgcatca ccagccgatt gtcacaaatc accaaaaccc cgcgatcgtc
    10981 ggcgtcgcga atcagtcgcc ctaccccctg cttgagagta atcaccgcat ccggcagttg
    11041 tacttcatcg aacgggtcgc caccgcgcaa acggcaatct tccatgcgcg cttttaacag
    11101 cgggtcatcc ggtgaggtaa acggcaactt gtcgataatc accaatgaca atgtatcgcc
    11161 acgcacgtcc accccttccc agaaactact ggttgccaca agaagcgcgt taccggcgct
    11221 gacaaattgc tgcaacagtt gccctttgct ggtttccccc tgcaacaaaa cgggaagcgt
    11281 catggtagcg cggaactgct cggccagatc acgcatcatg gcgtgcgagg tacaaagcat
    11341 aaaacaacga ccgttgttag cttcgatgat cggtcgcagc attgccgcta actggcgagc
    11401 agaacctggc tggttggttt gcggcagatt gcgcggcaca cagagtaacg cctggcggct
    11461 gtaatcaaac gggcttggca atagcaacga ctccgcctgt tctatcccaa gccgcgaggt
    11521 gaaatgatgc agatcgtcgt tcaccgacag cgttgctgag gtgaagatcc agctaccagg
    11581 cttttgcgcc attaactctt tgaatttatc cgccacgctg agcggcgtga gagccagagt
    11641 aaaatggcgc gaagtgcatt cgtaccagta gctgtagccc ggctgattga tctcttttag
    11701 ccgcttcagc cgtgtgcgat acaacgtagc gcgctcaaat gccgcatcca gcaaggcgga
    11761 acgccccagt gacagtttcg ccacgtcata acaaagttcc agggtgtcat cgagcagtaa
    11821 aaatgcccgc tgaatttgcg gattagctaa cagctcacgc aggttaccgc gataacctgg
    11881 ctcaccgagt tgcagacgaa aatcctgcgc actctgggca agacgatcag cgcacttttg
    11941 taactgctgg gtgtctttta attcggtgcg gtaggcgatg gtgatgtctt ttgccaggtc
    12001 gagcagttgt cgactggaga gtgactgacc aaaatactgg ctggcaatgt ccggtagctg
    12061 gtgggcttcg tcgaagatca tgacgtccgc ttccgggatc agttcgccaa atccactctc
    12121 tttaaccacc atatccgcca gaaagagatg atggtttacc accaccacat cggcgtccat
    12181 cgctttttta cgtgctttga ccacaaagca atctttatac atcgggcagt cgctgccaag
    12241 acagttgtcg ttggtgctgg taaccagcgg ccacgcctgt gaatcttccg ccacgctgac
    12301 gcaggtgctg atatcaccat cgactgtttg attagaccag gagcgcagca ggatcacatc
    12361 gcttaagatt tgtaccggca gatcgccccc cgccagcgcc tgctgttcga gacgttcgag
    12421 gcagaggtag tttgagcgcc ctttcagcag cgccacgttg cccgtatatt tcaatgcctt
    12481 tgagactgtt ggcaaatcgc ggctgtagag ctgatcctgc aacgcttttg agccggtcga
    12541 gataatgact ttctttttcg cccgcagcgc aggagccagg taagcgtagg ttttgcccgt
    12601 accggttcct gcttccacca ccagcggctg gcctttttct atcgcctggg tgacggctac
    12661 cgccatctgt cgctgtggtt ctcgcggctt aaagcctggt atcgctttcg ccagctgacc
    12721 gtctggtgca aaatcgtccg tcacactacc ccctgttgat ttgaacgggg attatgtcag
    12781 gatgagggcg ctttcgccag ttgaagtggt gacggcaacc tcacattgtg gcagtctttg
    12841 cagcacgaaa atgaaagtat aatgaggaca aaatgatgac tatcgttcgt atcgatgctg
    12901 aagcccgctg gtctgatgta gtaatccaca acaacacgct ctactacact ggtgtaccgg
    12961 aaaacctcga cgccgatgct tttgagcaaa ccgccaacac gctggcgcag attgacgccg
    13021 tgctggaaaa acagggcagc aataaatcga gcattctgga tgccaccatt ttcctggctg
    13081 ataaaaacga cttcgcggcg atgaataaag cgtgggatgc ctgggttgtg gcgggtcatg
    13141 cgccggtgcg ctgcacggtg caagcggggc tgatgaaccc gaagtataaa gttgaaatta
    13201 agattgtggc tgcggtgtaa gctttatcga agcaaaataa gtcagacgat catttatcga
    13261 taatactggt cggttttaca taaatcgacc agagattaag atattactgt tgatgctcct
    13321 gctaattcat tatttatttt tataatttca attttagcta taaagcta
//
LOCUS       DAZPEB010000112        13269 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZPEB010000112 DAZPEB010000000
VERSION     DAZPEB010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13269
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.113"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(1..165)
                     /gene="yojO"
                     /locus_tag="ACHURJ_002723"
     CDS             complement(1..165)
                     /gene="yojO"
                     /locus_tag="ACHURJ_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HFR1281607.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            274..768
                     /gene="napF"
                     /locus_tag="ACHURJ_002724"
     CDS             274..768
                     /gene="napF"
                     /locus_tag="ACHURJ_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFR1281608.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            758..1021
                     /gene="napD"
                     /locus_tag="ACHURJ_002725"
     CDS             758..1021
                     /gene="napD"
                     /locus_tag="ACHURJ_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFR1281609.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            1018..3504
                     /gene="napA"
                     /locus_tag="ACHURJ_002726"
     CDS             1018..3504
                     /gene="napA"
                     /locus_tag="ACHURJ_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFR1281610.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTLITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEDLLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTQDATDPLSKETDFKKCAVKLEKV"
     gene            3511..4206
                     /gene="napG"
                     /locus_tag="ACHURJ_002727"
     CDS             3511..4206
                     /gene="napG"
                     /locus_tag="ACHURJ_002727"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFR1281611.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHRDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            4193..5056
                     /gene="napH"
                     /locus_tag="ACHURJ_002728"
     CDS             4193..5056
                     /gene="napH"
                     /locus_tag="ACHURJ_002728"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFR1281612.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVVGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNCCMDCFHVCPEPRVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            5053..5502
                     /gene="napB"
                     /locus_tag="ACHURJ_002729"
     CDS             5053..5502
                     /gene="napB"
                     /locus_tag="ACHURJ_002729"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFR1281613.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQERMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            5512..6114
                     /gene="napC"
                     /locus_tag="ACHURJ_002730"
     CDS             5512..6114
                     /gene="napC"
                     /locus_tag="ACHURJ_002730"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFR1281614.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            6127..6750
                     /gene="ccmA"
                     /locus_tag="ACHURJ_002731"
     CDS             6127..6750
                     /gene="ccmA"
                     /locus_tag="ACHURJ_002731"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFR1281615.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            6747..7409
                     /gene="ccmB"
                     /locus_tag="ACHURJ_002732"
     CDS             6747..7409
                     /gene="ccmB"
                     /locus_tag="ACHURJ_002732"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFR1281616.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            7451..8188
                     /gene="ccmC"
                     /locus_tag="ACHURJ_002733"
     CDS             7451..8188
                     /gene="ccmC"
                     /locus_tag="ACHURJ_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFR1281617.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            8185..8394
                     /gene="ccmD"
                     /locus_tag="ACHURJ_002734"
     CDS             8185..8394
                     /gene="ccmD"
                     /locus_tag="ACHURJ_002734"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFR1281618.1"
                     /translation="MTLAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILCGVEQQRAREARLRAAQQQEAA"
     gene            8391..8870
                     /gene="ccmE"
                     /locus_tag="ACHURJ_002735"
     CDS             8391..8870
                     /gene="ccmE"
                     /locus_tag="ACHURJ_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFR1281619.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            8867..10810
                     /gene="ccmF"
                     /locus_tag="ACHURJ_002736"
     CDS             8867..10810
                     /gene="ccmF"
                     /locus_tag="ACHURJ_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFR1281620.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            10807..11364
                     /gene="dsbE"
                     /locus_tag="ACHURJ_002737"
     CDS             10807..11364
                     /gene="dsbE"
                     /locus_tag="ACHURJ_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFR1281621.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            11361..12413
                     /gene="ccmH"
                     /locus_tag="ACHURJ_002738"
     CDS             11361..12413
                     /gene="ccmH"
                     /locus_tag="ACHURJ_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFR1281622.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPDAFPEQSVPEGKRAGYIVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLSANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(12448..13095)
                     /gene="narP"
                     /locus_tag="ACHURJ_002739"
     CDS             complement(12448..13095)
                     /gene="narP"
                     /locus_tag="ACHURJ_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFR1281623.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPSFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(13088..>13269)
                     /locus_tag="ACHURJ_002740"
     CDS             complement(13088..>13269)
                     /locus_tag="ACHURJ_002740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1281624.1"
                     /translation="EADAKFRLYNENDAKANDKVVVFTTCPYIKWGNIHAPGSVTNAT
                     LKHNDSNKTPGDYYA"
BASE COUNT         2787 a         3364 c         3933 g         3185 t
ORIGIN      
        1 ttaaattctg atgagagaac aaaccagcag aaatatctct ggtgaattaa tcgctatata
       61 aatatgttta taaccatttg aaatgtgagc aaaagcccgt ttttgccacg cttcccgcca
      121 ctcttttgat cctgctacag gttttacccc gatcggggta tgcatctttg acacatcctt
      181 taatatctta gcggttataa aaatggctta ttaattatgc ggtttatttg gtcgctctca
      241 attttcagag cgcgttaatg atggaaggtc aatgtgaaga ttgatgcatc ccgtcggggc
      301 atactcactg gtcgctggcg caaagccagt aacggtatcc gtccgccctg gtccggtgat
      361 gaatctcatt ttctgaccca ttgcacccgt tgcgacgcct gtattaatgc ttgtgaaaac
      421 aacattctgc aacgcggcgc gggcggctat ccgagcgtta atttcaaaaa taacgagtgc
      481 agcttctgtt acgcttgcgc gcaggcttgc cccgaatcgc tattttctcc gcgccacacc
      541 agggcctggg atttgcagtt caccattggg gacgcgtgcc tggcgtatca gtcagttgaa
      601 tgccgccgct gtcaggatag ctgtgagcca atggcaatta tctttcgccc gacgctctcc
      661 gggatctacc agccgcaact taatagccaa ctttgcaacg gatgtggagc atgtgcagcc
      721 agctgtccgg tatcagccat aactgcggag tatctccatg cacactaact ggcaagtttg
      781 cagcctggtc gtgcaggcca aaagcgaacg aatttcagac atcagcaccc aactgaacgc
      841 ctttcccggc tgtgaagttg ctgtcagcga cgcgccgagc ggtcagttga ttgtggtggt
      901 ggaagcagaa gacagcgaaa cgctgatcca aaccattgag tcagtacgca acgtagaggg
      961 cgtgctggcg gtgtcgctgg tttatcacca gcaggaagag caaggtgagg aaacaccatg
     1021 aaactcagtc gtcgtagctt tatgaaagct aacgccgttg cggccgctgc ggcggctgcc
     1081 ggtctcagcg tgccgggcgt tgcccgcgcc gttgttggtc agcaggaagc catcaaatgg
     1141 gataaagcgc cgtgccgttt ctgcggtact ggttgcggcg ttctggtcgg aacgcagcag
     1201 ggacgtgtgg tggcctgtca gggcgaccca gacgcaccgg ttaaccgtgg cctgaactgc
     1261 attaagggct atttcctgcc caagatcatg tacggtaaag accgtttgac gcagccgctg
     1321 ctgcgtatga aaaacggtaa atatgacaaa gaaggcgaat ttaccctaat cacctgggat
     1381 caggccttcg atgtgatgga agagaaattc aaaaccgccc tgaaagaaaa agggccggaa
     1441 tcgatcggta tgttcggttc tggtcagtgg actatctggg aaggttatgc cgcgtccaag
     1501 ctgtttaaag cgggcttccg ttcgaacaac atcgacccga acgcgcgtca ctgtatggcg
     1561 tcggcagtag ttggctttat gcgtaccttt ggtatggatg agccgatggg ctgctatgac
     1621 gacatcgagc aggctgacgc gtttgtgctg tggggcgcaa acatggcgga gatgcacccg
     1681 atcctctggt cgcgcatcac taaccgtcgt ctctctaatc agaacgtcac cgtggcggtg
     1741 ctttctacct accagcatcg tagcttcgag ctggcggata acggcatcat ctttacgcca
     1801 cagtctgacc tggtgatcct gaactacatc gccaactata tcattcaaaa caatgcgata
     1861 aatcaggact tcttcagcaa acacgttaac ctgcgcaaag gggcgacgga cattggctac
     1921 ggtttacgtc cgacccatcc gctggaaaaa gcagcgaaga atccaggttc tgacgcctcc
     1981 gaaccgatga gctttgaaga ttacaaagcc ttcgttgccg agtatacgct ggaaaaaact
     2041 gccgaaatga ccggcgtgcc gaaagaccag ttagaacaac tggcgcagct gtatgccgat
     2101 ccgaacaaga aagtcatctc ctactggacg atgggcttca accagcatac tcgtggcgtg
     2161 tgggctaaca acctggtcta caacctgcac ctgctgaccg gcaaaatttc ccagccgggt
     2221 tgcggtccgt tctccctgac cgggcaacct tctgcgtgtg gtactgcgcg tgaagtgggc
     2281 acctttgctc accgtctgcc tgcggacatg gtggtgacca acgagaaaca ccgtgatatc
     2341 tgcgagaaga agtggaatat cccgagcggc accattccgg cgaaaatcgg tctgcacgcg
     2401 gtggcacaag accgtgcgct gaaagacggc aaactgaatg tttactggac catgtgtacc
     2461 aacaacatgc aggccgggcc gaacatcaat gaagagcgta tgccgggctg gcgcgatccg
     2521 cgcaacttca tcatcgtctc cgatccgtat ccgacagtca gtgcgctggc cgccgacttg
     2581 atcctgccga ccgcaatgtg ggtagagaaa gagggcgctt acggtaacgc cgaacgccgt
     2641 actcagttct ggcgtcaaca ggtacaggca ccgggcgaag cgaaatcgga tctctggcag
     2701 ttagtccagt tctcccgccg cttcaaaact gaagaagtat ggccggaaga tctgctggcg
     2761 aagaaaccgg aactgcgtgg caaaacgctg tacgaagtgc tgtatgccac accggaagtg
     2821 agcaaattcc cggtatccga actggcggaa gatcagctga acgatgaatc ccgcgagctg
     2881 ggcttctatc tgcaaaaagg gctgttcgaa gagtacgcat ggtttggtcg cggtcacggt
     2941 cacgatctgg caccgttcga tgactaccac aaagcgcgcg gtctgcgctg gccggtggtg
     3001 aacggtaaag aaacgcagtg gcgttacagc gaaggtaacg acccgtacgt gaaagcgggc
     3061 gaaggctata agttctacgg caaaccggat ggcaaagcgg tgatcttcgc actgccattc
     3121 gaaccggcgg cggaagcacc ggatgaagag tacgacctgt ggctctctac cggacgcgtt
     3181 ctggagcact ggcacaccgg cagtatgact cgccgtgtac cagaactgca ccgcgccttc
     3241 ccggaagcgg tcctgtttat tcacccgctg gatgcgaaag cgcgcgatct gcgtcgtggc
     3301 gacaaagtga aagtggtttc tcgccgtggc gaagtgatct cgattgtcga aacgcgcggt
     3361 cgtaaccgtc cgccgcaggg cctggtgtac atgccgttct tcgacgccgc acagctggtt
     3421 aacaaactaa cgcaggacgc gaccgatccg ctctcgaaag agacggactt caagaagtgc
     3481 gcagtcaaac tggagaaggt gtaagccatt atgtcccggt cagcgaaacc tcaaaatggt
     3541 cgccgccgct ttctgcgcga tgttgttcgc acagcaggcg ggctggctgc cgtgggtgtg
     3601 gcgctggggt tacaacagca aaccgcacgc gcatctggcg tgcggttgcg cccgcccgga
     3661 gccataaacg agaacgcctt tgccagtgcc tgtgtgcgtt gcggtcagtg tgttcaggct
     3721 tgcccttacg acaccttaaa actggcgacg ctggcctctg gtctgtcggc gggcacgcca
     3781 tattttgtcg cacgggatat tccttgcgaa atgtgtgagg acattccgtg cgccaaagtg
     3841 tgcccgagcg gtgcgctgga tcgtgagatc gaatcgatcg acgacgcgcg gatggggctg
     3901 gcggtactgg tggaccagga aaactgtctc aactttcagg ggctgcgctg cgatgtttgt
     3961 tatcgcgaat gcccgaaaat tgatgaggcc atcaccctgg agctggagcg caacacgcgt
     4021 accggtaagc acgcccgctt tctgccgacg gttcacagag acgcctgtac cggttgcggt
     4081 aagtgcgaaa aagtgtgcgt gctggaacaa ccggcaatca aggtgttacc gctgtcactg
     4141 gctaaagggg agttaggtca ccattaccgc ttcggctggc tggaggggaa caatggcaaa
     4201 tcgtaaacgt gacgccgggc gcgaggcgct ggagaaaaaa ggctggtggc gcagtcaccg
     4261 ttggctggtg ttacgtcgcc tttgtcagtt cttcgtgctg gggatgtttt tgagcggtcc
     4321 gtggtttggt gtgtggatct tacacggtaa ctacagcagt agcctgttat tcgacaccgt
     4381 tccgctgacc gatccgctga tgacgctgca aagtcttgcc agcggtcatc tgcctgccac
     4441 ggtggcgttg accggtgcgg tgattatcac cgtgctctac gccctggcag gaaagcgatt
     4501 attttgcagc tgggtctgcc cgctgaatcc gattaccgac ttagcgaact ggttacgcag
     4561 gcgctttgac ctcaatcagt ctgcgacgat ccctcgccac attcgctacg tgctgttggt
     4621 ggtcattctg ctgggatcgg ccttaactgg cacgctcatc tgggaatgga tcaaccccgt
     4681 atctctgatg gggcgcagcc tggtagtggg cttcggcagc ggcgcgctgc ttattctcgc
     4741 gctgttttta tttgatctac tggtcgttga acacggctgg tgcgggcaca tttgtccggt
     4801 tggcgcgctg tatggcgtgc tgggtagcaa aggtgtgatt accgttgcgg ccaccgatcg
     4861 gcagaaatgt aactgctgta tggattgttt tcatgtttgc ccggaaccgc gtgtgctacg
     4921 tgccccggtg ctggatgaac aaagcccggt gcaggtcacc agccgcgatt gcatgacgtg
     4981 cggtcgctgc gtggatgtct gttctgagga tgtatttaca ataactacac gatggagttc
     5041 gggagcgaaa tcatgaaaag ccatgacctg aagaaagcgc tgtgtcaatg gacggcgatg
     5101 ctggccctgg tggtaagcgg cgcggtttgg gcggctaatg gcgtcgattt tagccagtcg
     5161 ccggaagtat ccgggacaca ggaaggggcc attcggatgc caaaagagca ggaacggatg
     5221 ccgctgaact atgtgaatca gccgccaatg atcccgcata gcgttgaagg ttatcaggta
     5281 acgaccaata ccaaccgctg cttgcagtgc cacggtgtcg aaagttatcg caccactggc
     5341 gcgccgcgca ttagtcctac tcactttatg gacagcgacg gcaaagtggg cgcggaagtg
     5401 gctccgcgtc gttatttctg tctgcaatgt cacgtaccgc aggccgatac cgcgccaatc
     5461 gtggggaata cctttacccc atcaaaaggt tacgggaaat aagaggttat tatgggaaat
     5521 tctgaccgta agcctggtct gattaagcgc ctgtggaaat ggtggcgtac ccccagccgt
     5581 ctggcgctgg ggacgctgct gttgatcggt tttgttggcg gcatcgtctt ctggggtggc
     5641 tttaacaccg ggatggaaaa agccaatacc gaagagttct gcattagctg ccacgaaatg
     5701 cgcaacacgg tatatcagga atacatggat tccgtgcact acaacaaccg tagcggcgtc
     5761 cgtgcgacct gtccggattg tcacgttccg cacgagtttg tgccgaagat gatacgcaag
     5821 ctcaaagcaa gtaaagagct gtatggtaaa atttttggcg ttattgacac gccgcagaaa
     5881 tttgaagctc atcgtctgac gatggcacag aatgagtggc ggcgcatgaa ggacaataac
     5941 tcgcaggagt gccgtaactg tcacaacttc gagtatatgg atacaaccgc ccagaaatcg
     6001 gttgccgcga agatgcatga ccaggcggtg aaagatgggc aaacctgtat tgattgccat
     6061 aaagggatag cgcacaagct gcccgatatg cgtgaagtcg agccaggttt ttaacagggt
     6121 tattgcgtgg gtatgcttga agccagagag ttactttgtg agcgggatga acgaacctta
     6181 tttagtggct tgtcatttac gctgaacgca ggagagtggg tacaaatcac cggtagcaac
     6241 ggcgcgggga agacaacgct tctccgtttg ctgacggggt tgtctcgccc tgacgcaggc
     6301 gaggttctct ggcaagggca gcccttgcat caggtacgcg acagctacca tcaaaacctg
     6361 ttatggatag gccatcagcc ggggatcaaa acccggctga cggcgttaga aaatctgcac
     6421 ttttatcatc gcgatggcga taccgcacaa tgtctggaag ccctggcgca ggccgggctt
     6481 gccggattcg aagatattcc tgtaaatcag ctctcggccg ggcaacaacg ccgcgtcgct
     6541 ttagcgcgtc tgtggctgac ccgtgccacg ttatggatcc tcgacgagcc ttttaccgcg
     6601 attgacgtta acggcgtcga tcgtctgacc cagcgtatgg cgcagcatac ggagcagggg
     6661 gggattgtga ttctgactac ccaccagccg ctcaacgttg ctgaaagtaa aattcgccgc
     6721 atttcactga cgcaaacgag ggccgcatga tgttctggcg cattttccgt cttgagctgc
     6781 gtgtagcgtt tcgccatagc gccgaaatcg ccaacccgct gtggttcttc ctgattgtaa
     6841 ttaccctttt tccgctcagt atcggtccgg agccgcaact gctggcgcgt attgcaccgg
     6901 gcattatctg ggttgctgcg ctgctttcat ccttgctggc gctggaacga ctgttccgtg
     6961 acgatttgca ggacggcagt cttgaacaat tgatgttgtt gccgttaccc ttgcccgccg
     7021 ttgtgctggc gaaggtgatg gcgcactgga tggtaaccgg tctgccgtta ctcatccttt
     7081 cgccactggt agcaatgcta ctgggaatgg atgtttatgg ctggcaagtg atggcgctga
     7141 cgctgctgct gggaacgcct acgcttggct ttctcggtgc accgggtgtg gcgttgacag
     7201 tgggacttaa gcgcggtggt gtgctgctca gcatactggt gttaccgctg actatcccat
     7261 tactcatctt tgccaccgcc gcgatggacg cggcttccat gcatttgccc gttgacgggt
     7321 atctggcaat tttaggcgcg ttgctggcag gcaccgcgac attaagtcct tttgcgacgg
     7381 cggcagcgtt acgaatcagc attcaataac gctggattac tgatttttgt tcgagtctgg
     7441 tatcgaaact atgtggaaaa cactgcatca actggcgatc ccaccacggc tgtatcaaat
     7501 ctgtggctgg tttataccgt ggctggcaat tgccagtgtg gtcgtgctta ccgtcggctg
     7561 gatctgggga ttcggctttg ctccggctga ttatcagcag ggaaatagct accgcattat
     7621 ctacctgcat gtgcctgcgg cgatctggtc gatgggtatt tatgcatcaa tggcggtggc
     7681 ggcgtttatt ggccttgtct ggcagatgaa aatggccaac ctggcggtgg cggcgatggc
     7741 ccccattggt gccgtgttta cctttattgc cctggttacc ggctctgcat ggggaaaacc
     7801 gatgtggggc acctggtggg tatgggatgc acgtctgact tctgaactgg tgctgctgtt
     7861 tttgtatgtg ggtgtgattg ccctgtggca cgccttcgac gaccgccgtc tggcgggccg
     7921 tgcggcaggt atcctggtgc tgattggcgt ggtgaatctg ccgattattc attactccgt
     7981 ggagtggtgg aacaccctgc atcagggatc aacgcggatg cagcaaagta tcgatccggc
     8041 gatgcgttcg ccgctgcgct ggtcgatttt tggcttcctg ctcctgtctg ccacgctgac
     8101 gctgatgcgg atgcgtaatt tgattttgct gatggaaaaa cgccgtccgt gggtgagtga
     8161 actgatactg aaaagaggcc gtaaatgacc cttgcatttg cttcctggaa tgaatttttc
     8221 gcaatgggcg gttacgcctt ttttgtctgg ctggcggtgg tgatgaccgt tattccgctg
     8281 gtggttttgg tcgtgcactc ggtgatgcag catcgcgcaa ttctgtgtgg tgtggagcaa
     8341 cagcgggcgc gtgaggcgcg tttacgtgct gcgcaacagc aggaggctgc atgaatattc
     8401 gccgtaaaaa ccgcttgtgg attgcctgtg ctgtgttggc aggtttggcg ctgaccatcg
     8461 gtctggtgct gtatgcgctg cgctcgaata tcgatctctt ttatacgccg ggggaaattc
     8521 tctacggcaa gcgtgaaact cagcaaatgc cggaagtcgg tcagcgtctg cgcgttggcg
     8581 ggatggtgat gccgggtagt gtgcagcgcg atcccaattc gctgaaagtg accttcacca
     8641 tttacgacgc tgaaggctca gtggatgtct cttacgaagg cattttgccg gatctgttcc
     8701 gtgaagggca gggcgttgtg gtgcagggcg agctggaaaa aggcaatcat atcctcgcga
     8761 aagaagtgct ggcgaaacac gacgaaaact acacgccgcc agaagttgag aaagcgatgg
     8821 aagctaacca ccgtcgcccg gcgagtgttt ataaggaccc agcatcatga tgccggaaat
     8881 tggaaacggg ctgctgtgcc tggcgctggg aattgcgctg ctgctgtccg tgtatccgct
     8941 atggggcgtg gcgcgcggag atgcgcgcat gatggcgtct tcccgcttgt ttgcctggct
     9001 gctgtttatg tctgtggctg gcgcatttct ggtgctggtc aatgctttcg tggtcaacga
     9061 ctttaccgtc acctatgttg ccagcaactc caatacccag cttccggtgt ggtatcgcgt
     9121 ggcggctacc tggggcgcgc atgaaggttc gcttctgctg tgggtgctgc tgatgagcgg
     9181 ctggaccttt gcggtggcga tttttagtca gcgtattccg ctggatattg tggcccgtgt
     9241 actggcgata atggggatgg tcagcgtcgg ctttttgctg ttcattctct ttacctctaa
     9301 cccgttctca cgcacgctgc cgaacttccc aattgaaggg cgcgatctta acccgctgtt
     9361 acaggatccg gggctgatct tccatccgcc tctgctttat atggggtacg tgggtttctc
     9421 ggtggcgttt gcttttgcca ttgcttcttt gctgagcggg cgtctggaca gcacttatgc
     9481 gcgttttact cgcccgtgga cgctggcggc gtggattttc ctgacgctcg gcattgtgct
     9541 cggttccgca tgggcctatt acgaactcgg ctgggggggc tggtggttct gggatccggt
     9601 agaaaacgcc tcgtttatgc cgtggctggt ggggactgcg ctgatgcact cactggcggt
     9661 cactgaacaa cgcgccagct tcaaagcgtg gacattactg ctggcaatca gtgccttctc
     9721 gttgtgtctg ctggggactt tcctggtgcg ttccggcgtg ctggtatcgg tacacgcgtt
     9781 tgcctctgat ccggcacgcg gtatgtttat cctcgccttt atggtactgg tgattggtgg
     9841 ttcgctgctg ctgtttgccg cgcgtggaca caaagttcgc tcacgcgtaa acaatgcgct
     9901 gtggtcgcgg gaatctctgc tgttagcgaa caacgttttg ttggtcgccg cgatgctggt
     9961 ggtattactg gggacgctgc tgccgctggt gcacaagcaa ctgggactgg gcagtatttc
    10021 gattggcgaa ccgttcttca acaccatgtt tacctggctg atggtgccgt ttgcgctgct
    10081 gcttggtgtc ggtcctctgg tgcgctgggg gcgcgatcgc ccacgtaaaa tccgcaattt
    10141 attgattatc gccttcatct caacgctggt gctgtcgctg ctgctgccgt ggctgttcga
    10201 aagcaaagtt gtggcgatga cggtgctcgg cctggcaatg gcctgctgga ttgctgtgct
    10261 ggcaattgcg gaagctgcgc tgcgtatttc acgtggcacg aaaaccacct tcagttattg
    10321 gggaatggtg gcggctcacc tggggctggc agtgacaatt gttggtattg cctttagcca
    10381 gaactatagc gttgagcgtg atgtgcgcat gaagtccggc gatagcgtcg atattcatga
    10441 atatcgcttc accttccgtg atgtcaaaga ggtgactggc ccgaactggc gcggcggtgt
    10501 ggcgactatt ggcgtaacgc gagagggtaa accggaaacg gtgctgtatg cggaaaaacg
    10561 ttattacaac actgccgggt cgatgatgac cgaagcggcg attgacggcg gcatcacgcg
    10621 tgacctgtac gccgcgctcg gtgaagagct ggaaaacggc gcgtgggccg tgcgtcttta
    10681 ctacaaaccg tttgttcgct ggatttgggc gggcgggctg atgatggcgt tgggcggact
    10741 gctgtgtctg tttgatcctc gctatcgtaa gcgcgtgagt ccgcaaaaaa ctgcgccgga
    10801 ggctgtatga agcgcaaagt attgttaatt ccgttgatta tcttcctggc gattgccgcg
    10861 gcgctgctgt ggcagctggc gcgtaatgcc gaaggggatg atccgaccaa tctggaatcg
    10921 gcgctcattg gcaagccagt gccgaagttt cgcctcgaat cactggacaa tccggggcag
    10981 ttttatcagg cggatgtgct gactcagggc aaacctgtgc tgcttaacgt ttgggctacc
    11041 tggtgtccga cctgccgtgc ggaacatcaa tatctgaatc agctttctgc gcagggcatt
    11101 cgcgtggtcg gcatgaacta taaagacgat cgccagaagg caataagctg gctgaaagag
    11161 ctgggcaatc cttacgcgct aagcctgttt gatggcgatg gcatgttagg gctggatctc
    11221 ggtgtctatg gcgcgccaga aacgttcctc atcgacggca acggcatcat tcgctatcgc
    11281 catgcgggcg atctcaatcc tcgcgtctgg gaagaagaga tcaagccgct gtgggagaaa
    11341 tacagtaagg aggccgcaca atgaggtttt tattgggcgt gctgatgctg atgatctccg
    11401 gctcagcgct ggcgaccatc gacgtgttgc agtttaagga tgaagcgcag gagcagcagt
    11461 tccgccaact cactgaagaa ctacgctgcc cgaaatgcca gaacaacagc attgccgatt
    11521 ccaactcgat gattgccacc gacctgcgcc agaaagtgta tgaactgatg caggaaggta
    11581 aaagtaaaaa agagattgtc gattatatgg tggcgcgtta cggcaacttc gtcacttacg
    11641 atccgccgtt aacgccgctg accgtgctgc tgtgggtgct tccggtagtg gctattggca
    11701 ttggcggttg ggtcatttac gcccgttcgc ggcgtcgggt acgcgtggtg ccggacgcgt
    11761 ttcctgaaca aagcgtgccg gaaggtaagc gtgccggata tattgtttat ctgccgggta
    11821 ttgtggtggc gttaattgtg gctggcgtca gctactacca gactggcaat tatcagcagg
    11881 tgaaaatctg gcagcaggcc acggcacagg ctccggcgtt gctggacagg gcgctggatc
    11941 cgaaagccga tccgctcaac gaagaagaga tgtcgcgtct tgcgctgggg atgcgtactc
    12001 aactgcaaaa aaatccggga gatatagaag gctggattat gttgggccgc gttggcatgg
    12061 cgctgggtaa cgccagtatc gccaccgatg catacgctac tgcgtatcgc ctcgatccga
    12121 aaaacagtga tgccgcactg ggttatgctg aagcgttgac acgttcatct gatcccaacg
    12181 acaaccgcct cggtggtgaa ctgctgcgcc agttggtgag aacggaccat agcaatatcc
    12241 gtgtgttaag catgtatgcg tttaatgcct ttgagcagca gcgatttggc gaagccgttg
    12301 ccgcgtggga gatgatgttg aaactcttat ctgccaacga tactcgccgt gcggtgattg
    12361 aacgtagtat cgcgcaggcg atgcaacatt tgtcgccgca ggagagtaaa taaaaagagg
    12421 cccgatggtg atgccatcgg gctattttta ttgtgccccg cgttgttgca ggaacagaat
    12481 ggtggctgcc acgcgtgagc ggacattgag tttgcgcagc agattgcgaa tatgtacttt
    12541 tactgtctgc tctgaaatat tcaacaccga ggcaatctgt ttatttgaca gcccctgtgc
    12601 cagctcgtgc agaacatcca gctcgcgctc cgtcagcacg ctgaagggat cttcttccgc
    12661 gccaaacatt tcacgttcac gtaagtactg attgacgcgt tcgctaaaga ctttgctgcc
    12721 tttcgctcca gcacgaattg cttccagcaa tacttccggg tcgctgtctt tcaacagata
    12781 accgtctgcg cctgcgtcta tcagcgcaaa gacatcgctg gaggcatctg atacggtcag
    12841 gataataatt tgcgcggtaa cgccatccct gcgcaaggca ttgagggtat ccaggccact
    12901 catacctttc atattgagat ccagcaagat cacgtcgata tccagtctat ttgccagatc
    12961 gatcgcgctc gcgccgtcgc ccgcttcggc gaccacttca aagctaggat caagctccag
    13021 taactgacga acaccgcgtc gcataagtgg atgatcatcc acaatcatca cctgaaaagg
    13081 cgttgcttca ggcatagtag tctcctgggg ttttattaga atcattatgt ttgagcgtag
    13141 cgttggtaac gctaccaggc gcgtgaatgt taccccattt tatgtatgga catgtagtga
    13201 aaactacaac cttgtcgttc gctttggcat cattttcatt ataaagacgg aattttgcat
    13261 cagcttctc
//
LOCUS       DAZPEB010000113        13114 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZPEB010000113 DAZPEB010000000
VERSION     DAZPEB010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13114
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.114"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..619)
                     /locus_tag="ACHURJ_002741"
     CDS             complement(<1..619)
                     /locus_tag="ACHURJ_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFR1281625.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSR"
     gene            complement(1007..1753)
                     /gene="cutC"
                     /locus_tag="ACHURJ_002742"
     CDS             complement(1007..1753)
                     /gene="cutC"
                     /locus_tag="ACHURJ_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFR1281626.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQWVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLNTLSNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(1767..2333)
                     /locus_tag="ACHURJ_002743"
     CDS             complement(1767..2333)
                     /locus_tag="ACHURJ_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR1281627.1"
                     /translation="MANWQSIDELQDIASDLPRFTHVLDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEYERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            2549..4282
                     /gene="argS"
                     /locus_tag="ACHURJ_002744"
     CDS             2549..4282
                     /gene="argS"
                     /locus_tag="ACHURJ_002744"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFR1281628.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWHKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(4324..4368)
                     /gene="yecV"
                     /locus_tag="ACHURJ_002745"
     CDS             complement(4324..4368)
                     /gene="yecV"
                     /locus_tag="ACHURJ_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecV"
                     /protein_id="HFR1281629.1"
                     /translation="MSIFRIHLDGNKKA"
     gene            4459..4947
                     /locus_tag="ACHURJ_002746"
     CDS             4459..4947
                     /locus_tag="ACHURJ_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFR1281630.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDIEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(5067..5459)
                     /gene="flhe"
                     /locus_tag="ACHURJ_002747"
     CDS             complement(5067..5459)
                     /gene="flhe"
                     /locus_tag="ACHURJ_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFR1281631.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(5459..7537)
                     /gene="flhA"
                     /locus_tag="ACHURJ_002748"
     CDS             complement(5459..7537)
                     /gene="flhA"
                     /locus_tag="ACHURJ_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFR1281632.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGCEQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(7530..8633)
                     /gene="flhB"
                     /locus_tag="ACHURJ_002749"
                     /pseudo
     CDS             complement(7530..8633)
                     /gene="flhB"
                     /locus_tag="ACHURJ_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
     gene            complement(8716..9360)
                     /gene="cheZ"
                     /locus_tag="ACHURJ_002750"
     CDS             complement(8716..9360)
                     /gene="cheZ"
                     /locus_tag="ACHURJ_002750"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFR1281633.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(9371..9760)
                     /gene="cheY"
                     /locus_tag="ACHURJ_002751"
     CDS             complement(9371..9760)
                     /gene="cheY"
                     /locus_tag="ACHURJ_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFR1281634.1"
                     /translation="MADKELKFLVVDDFSTMRCIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFDKLDM"
     gene            complement(9775..10713)
                     /locus_tag="ACHURJ_002752"
     CDS             complement(9775..10713)
                     /locus_tag="ACHURJ_002752"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HFR1281635.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPVGMLAMRQAGAWTLAQNEASCVVFG
                     MPREAINMGGVCEVVDLSQVSQQMLAKISAGQAIRI"
     gene            complement(10716..11528)
                     /gene="cheR"
                     /locus_tag="ACHURJ_002753"
     CDS             complement(10716..11528)
                     /gene="cheR"
                     /locus_tag="ACHURJ_002753"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020237614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFR1281636.1"
                     /translation="MRLPGCSRYSFRRISQLIYQRAGIVLADHKRDMVYNRLVRRLRS
                     LGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHHFPLLADHARRRSGEYR
                     VWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVLEKARSGIYRHEELKNL
                     TPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQYTVPGPFDAIFCRNVMI
                     YFDQNTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLRGQTVYALSKD"
     gene            complement(11666..12232)
                     /gene="flhC"
                     /locus_tag="ACHURJ_002754"
     CDS             complement(11666..12232)
                     /gene="flhC"
                     /locus_tag="ACHURJ_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFR1281637.1"
                     /translation="MSEKSIVQEARDIELITLGARLQMLESETQLSRGRLIKLYKELR
                     GSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLYLEQC
                     PQAEEGPLLALTRAWTLGRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLCQPPS
                     RAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(12235..12585)
                     /gene="flhD"
                     /locus_tag="ACHURJ_002755"
     CDS             complement(12235..12585)
                     /gene="flhD"
                     /locus_tag="ACHURJ_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFR1281638.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVNDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         3356 a         3510 c         3073 g         3175 t
ORIGIN      
        1 tgcgagaagt tttgaaagga taaatatgtg cgccattagt cactgaagta gcggcctgac
       61 tttccagctc atgcgccgcg ttgtcatcca tttttatttc tggcggaaaa tgggcaatgc
      121 ctttatgaca atcgatacag gtttcgccgc ccttttgtgc tttgttatgc attttctgcg
      181 cagattcact ttgcgaggca atatccatgg catcaaaaga atggcaacta cggcacgttg
      241 cagagtcagt tgcttttaat tctttccata ctgtttcggc catttcctgg cgatgagttt
      301 cgaacttatc gtcactgtct attttgccgc taacaaattc atgataaata tctttagatg
      361 cttttaattt agcaaataaa taatccatcc ctgactttgg aatatggcaa tctgcacatt
      421 ccgcacgtat ccctttctgg ttcgaaaagt ggacagttcc ctgatattcc tcaaaaggtt
      481 tactcatcga gtggcaggaa aggcaaaatg ctgtatccga cgttttatgt aaggcttttt
      541 gcgccagcaa taacccgccg ccaccaacca caaccgctat cagcaaaaac aataacccaa
      601 tgcgtttttt ccctcgcata gattcctcta aataaattcc agaacaccga aatcattgga
      661 taattaaata cacaacgaat taaccgcttg cataactcgg cacaacactg cctgaaataa
      721 tcgataaaga atatgattta ttacaatgta atcattaatt gctaaggaat aaccctgttg
      781 ttatttagaa tatatatttg tcagttttag caatttaaac cccataatta atgtgaatat
      841 ataaaatatt tttagcacaa cgctatgttc actgtcaccc gctcaacaga tcacggttat
      901 cgtacgtttt ttatactgct cagggataaa aaaaggcccc tgtcgaaatt gcaggggcct
      961 ggtacgagca agcatcatat tgggcgacat gatgcaacgg taaaaatcat ttggcctgat
     1021 ggcgttcaat gattcctttc atttcagcaa ccgccgcccc gtctacgaca taacgcgaat
     1081 actcgtccgc gtgtgcatct gatgacatgg acaatccttg attacgataa cgcatcggtg
     1141 aagcttgcca cgctcccgcg gagctatgga cttccagcac tccggcatcg aggaagtggt
     1201 gcaggttttc tgcacggact cctgctccgg ccataatgat tggagcatca cgatgggcaa
     1261 taagttccat aatttttgat aaaccttgca gtgcatctga tttttgccct gatgtcagca
     1321 cccgggcgat gcccaattcc gcaagattac tgagtgtatt taaagggtta gcgcacatat
     1381 cgaaggcgcg atgaaaagtc actgccagcg gaccggcagc agccattatt ttttccattc
     1441 gtggcatatc gacattcccg tcaacatcga gcacgcccgt caccagtcca ggaaaaccta
     1501 attcgcggac tgtgcgcaca tcctcaagaa tggcggcaaa ctcaccgtcg ctgtagcaaa
     1561 aatcaccacc gcgtgggcgg ataatcggat gcacagggat cgtcacccac tggcgcacgg
     1621 atttcagtac acccaacgac ggcgttaagc ccccctcttt tggggctgcg cataattcaa
     1681 ctctgtctgc gccgttttgc tgcgccgtta gtgcacattc catgctgtaa cagcaaattt
     1741 ccagtaatgc catttttact ccttaattac gccgactgct cgctggcaac gatctcttca
     1801 atggaccacg gatgaaactt aatagttgtt ttgccatcgg tgaccgccag cgtcggattc
     1861 ggtaatcgct catactcacc ttttggcgaa ctggttttca ctgagatccc cggtaggctg
     1921 agtccttcat cagaaagcaa tgccagcgca cgggcattca gcgtttctgg atcacccggc
     1981 agaacgattt caatatgttc ccaaccttcg tgtgggtaac gtttttcccc gggccacggt
     2041 agctccacaa tggaaaactg ccagtgcgca acctgtaccg gttcatgcaa tttaaacaga
     2101 caaatcggtc tgccattgat catattttct gacaaaagct cgccacactg ttcaaaaccg
     2161 cgacgccagc gctcagcggt gacgttttga tggcaacgca aagaaatgtg atcggcagtc
     2221 agcggagtga tatccaaccc cagacggcgg gagagttcat ctaatacgtg ggtaaatcgc
     2281 ggtaaatccg atgcaatatc ctgcagctcg tcgatagatt gccagttcgc cataatcact
     2341 cttcgtcttt cagtaaaagc gttaatttac cctgttgccc tgcgccaacc aaccgctgat
     2401 ttcacgccgc ttctgatgca atagtgaaaa cggcaatacg ccacgcgcac gttgctgacg
     2461 aaaacagcca tttgcagtat actcccgccc taatttcttt aactggtgcg ggcaattttt
     2521 gctcgcttca tcaatgtaag gtattccggt gaatattcag gctcttctct cagaaaaagt
     2581 ccgtcaggcc atgattgcgg caggcgcgcc tgcggattgc gaaccgcagg ttcgtcagtc
     2641 agcaaaagtt cagttcggcg actatcaggc taacggcatg atggcagttg ctaaaaaact
     2701 gggtatggca ccgcgacaat tagcagagca ggtgctgact catctggatc ttaacggtat
     2761 cgccagcaaa gttgagatcg ccggtccagg ctttatcaac attttccttg atccggcatt
     2821 cctggctgaa catgttcagc aggcgctggc gtccgatcgt ctcggtgttt ctacgccaga
     2881 aaaacagacc attgtggttg actactctgc gccaaacgtg gcgaaagaga tgcatgtcgg
     2941 tcacctgcgc tctaccatta ttggtgacgc agcagtgcgt actctggagt tcctcggtca
     3001 caaagtgatt cgcgcaaacc acgtcggcga ctggggcact cagttcggta tgctgattgc
     3061 atggctggaa aagcagcagc aggaaaacgc cggtgaaatg gagctggctg accttgaagg
     3121 tttctaccgc gatgcgaaaa agcattacga cgaagatgaa gagtttgccg agcgtgcacg
     3181 taactacgtg gtaaaactgc aaagcggtga cgaatatttc cgcgagatgt ggcacaaact
     3241 ggtcgacatc accatgacgc agaaccagat cacctacgat cgtctcaacg tgacgctgac
     3301 ccgtgatgac gtgatgggcg aaagcctcta caacccaatg ctgccaggaa ttgtggcgga
     3361 tctcaaagcc aaaggtctgg cagtagaaag cgaaggggcg accgtcgtat tccttgatga
     3421 gtttaaaaac aaggaaggcg aaccgatggg cgtgatcatt cagaagaaag atggcggcta
     3481 tctctacacc accactgata tcgcctgtgc gaaatatcgt tatgaaacac tgcatgccga
     3541 tcgcgtgctg tattacatcg actcccgtca gcatcaacac ctgatgcagg catgggcgat
     3601 cgtccgtaaa gcaggctatg taccggaatc cgtaccgctg gaacaccaca tgttcggcat
     3661 gatgctgggt aaagacggca aaccgttcaa aacccgcgcg ggtggtacag tgaaactggc
     3721 cgatctgctg gatgaagcct tggaacgtgc acgccgtctg gtggcagaaa agaacccgga
     3781 tatgccagct gacgagctgg aaaaactggc taacgcggtt ggtattggag cggtgaaata
     3841 tgcggatctc tccaaaaacc gcaccacgga ctacatcttc gactgggaca acatgctggc
     3901 gtttgagggt aataccgcgc catacatgca gtatgcttac acgcgtgtat tgtccgtgtt
     3961 ccgtaaggca gaaattaacg aagagcaact ggctgcagct ccggtaatca tccgtgaaga
     4021 tcgtgaagcg caactggcag ctcgcctgct gcagtttgaa gaaaccctca ccgtggttgc
     4081 ccgtgaaggc acgccgcatg tgatgtgtgc ttacctgtac gatctggctg gtctgttctc
     4141 tggcttctac gagcactgcc cgatcctcag cgcagaaaac gaagaagtgc gtaacagccg
     4201 tctgaaactg gcacaactga cagcgaagac gctaaagctg ggtctggata cgctgggtat
     4261 tgagacagta gagcgtatgt aatcgatttt tcgtgagagt aaagcctgat cagggttacc
     4321 tgatcaggct tttttattgc catctaaatg tattctgaaa atggacatgc cattgttttc
     4381 tgactgttgg ataagaggcc agaagcgtaa tatccggccc cagggaaacg ataacggttg
     4441 aatttaagga ataccgcagt gtttaaattt cttgtattaa cattaggcat tatctcttgc
     4501 caggcttacg cagaagatac ggttatagta aacgaccatg acatttcagc catcaaagat
     4561 tgttggcaaa aaaattcaga tgatgatact gacattaacg tgatcaaatc atgcctgcga
     4621 caagaataca atctcgtcga tgcgcaatta aataaagcct atggtgaagc ttatcgttat
     4681 atagaacaag tgccacgcac aggtgtaaaa aaacctgata tcgaacaact taacttgctt
     4741 aaaaaatcac agcgagcctg gctggatttt agggacaaag aatgtgaatt aatcctttca
     4801 aatgaggacg ttcaggattt aagtgaccct tattctgaat cagaatggct ctcatgtatg
     4861 atcatacaga ccaatacgcg aactcgccag ttgcagctat accgtaactc tgaagatttt
     4921 tatccaagcc ctttgacaag aggataattc acatcttttt ggcatgtttt gttgcaagct
     4981 attcctgata aataattgca acaagacatc gagccttttt cactgagtta ttaaacatac
     5041 tcgcgagcgc gtaatttttt tgtccttcag cgataattca caatcacttc attacgttgt
     5101 acctttagcg gtggaattaa ccgcccaccg cctggcactt cccagataaa tcgcaacggt
     5161 tctgctgccg ctataccgga aaaggccacg gtggttccgc tctgcccctc taattcgaca
     5221 caacgagatt gcgaacacaa gcgaacccgc agtcctgaag gtgtcgggcc gataagctga
     5281 taacgccacg ctaccagcgt catcaatcct gaagcaggtt gtcgcgtaga aagaggcgca
     5341 gacgacatcg actcaccgcg atgatttagc gtaattccta cactacttgc ctgccacatc
     5401 ccctccccgg cggcttgcac cagcagcgga aacaataata ttgctaataa ggttctcatt
     5461 atttgccgcc aattgtcgcc gtcatgcgga tatgtcggtt atcagacagt tccagattcg
     5521 acaggaccac taactgcggc aagctgcggc gcaggaagcg agacaataat ggtcgcagcg
     5581 cgtggttcac caacaatact ggcggcgcac ccagcatctc ctgacgggat agcgcttcct
     5641 gagtttgcgc cagtaaacga tccgccagcc ctggctccag tcctcccccg ccctgcagcg
     5701 cctgtagtaa caaacgttcc agcggtgtat cgaggccaat aacatggact tcatctttgc
     5761 caggaaacca ctgctgggta atcgcccgtc ccaacgccac gcgcacgacg gcggttaatt
     5821 catgtggatc gctttggatg ggcgcatgtt ccgccagcgt ttcgagaatg gtgcgcatat
     5881 cgcgaatcgg tactttttca tcgaggagat tttgcagcac tttatgcagt gtggtgagcg
     5941 tgacgacgcc aggaacgaga tcttccgtca gctttggcat ctcctgggcg acgcgatcca
     6001 acagctgttg cgcctcctga cgaccaaaca gctctgcggc atgctggcta atgaggtggt
     6061 taagatgcgt tgctaccacc gtgctggcct caaccactgt gtacccctga atctgcgcct
     6121 gttcttttag cgcactttca atccagatag cattcaggcc aaatgccgga tcgacggtcg
     6181 cctcaccagg taacgtcccg gcagcggttc cagggttaat cgccaaccag cgccccggat
     6241 aagcatcacc actgccaatc tccacgcctt tcatcaaaat gcgatagcgg gcaggttgca
     6301 gatccatatt gtcgcgaatg tgcaccactg gcggcagaaa tcccatctcc tgggcaaatt
     6361 tcttgcggat actgcgtata cggcccaaca actcaccatc ctgctggaaa tcgaccatcg
     6421 ggatcagtcg ataacccact tccattccca gagaatcttc cagttgtaca tcgttccacg
     6481 tcgcttcgac aacggtatta ttctctgcca tttttaccgg tttgggttcg gcaggcgctt
     6541 tttgttcgca tccgcgtatc caccaggcca gccccagcaa tccggcagtg aacagcaaaa
     6601 ataccaggtt cggcattcca ggcaccaagc cgagtaaacc gagcacggcg gcgcttaaca
     6661 acataacgct tgggttactg aaaagctgat tcaccatctg ctcgccaaca tcctgatcgg
     6721 tgctgacacg cgtaacgatg acccccgcgg cggtagaaat caccagcgcc ggaatttgtg
     6781 ccaccagacc gtcgccaatg gtcaatagcg tataactttc cgccgcgtgt cccatgctca
     6841 tgccatgttg cagcacgccg accagcaacc cgccgacaac gttaatgacc atgatgagga
     6901 tcccggcgat ggcatcgccg cgaacaaact tacttgcccc gtccattgag ccgtaaaaat
     6961 cggcttcctg agtcacttcg gagcggcgtt ttttcgcctc atcttcacca atcaatccag
     7021 cgttaaggtc ggcgtcaatc gccatctgct tacccggcat accatcgaga acaaagcgcg
     7081 cacccacttc tgcgatacgc ccggcacctt tggtaatgac cataaagttg atgatcacga
     7141 gaatgacaaa caccacgata cctatagcga aattgccacc aacgaggaag tgaccgaacg
     7201 cttcgaccac cttccctgcc gccgccgcgc cggtatgccc ttccatcaaa atgatacggg
     7261 ttgaagccac gttaagtgcc agacgcaaca acgtagtaaa caacagaatg gtcggaaacg
     7321 cagcaaactc aagcgtgcgc tgggtaaaca tcgccaccag caacaccatg atcgacaagg
     7381 caatattgaa ggtaaacaac aggtcgagta tgaatgcggg cagtggcagc accatcatcg
     7441 acaagatcaa caggatcaaa atcggtccgg caaggatctg ccattgtgtc gatttcaggt
     7501 ttgcgggcag gcgcagcatc gcggccagat tactcatggg tcggtttctc gttaataaaa
     7561 tccagggctt ccggcaccgg aagatgagta ggttgtacag ggcgctgtcc accagccaga
     7621 cgccagcgtt tcagttgcca gatccaggcc agcacttccg ccaccgcggc gtacagttga
     7681 cccgggattt gttgaccaat ctccgcatgt cgatacagcg ctcgcgccag cggcggcgct
     7741 tcaagcgtcg ggacgttatt ttcagcgcca atttcacgaa tgcgcagcgc gaccagccct
     7801 gcacctttag cgaccacttt cggtgcgctc attttgtttt cgtcatactg caacgctacc
     7861 gaatagtggg tcgggttatt gacaatgaca tccgctttcg gcacatcggc catcatccga
     7921 cgccgtgcgg cagctcgctg catctgacgg atccgtcctt taacatgggg gtcgccttcg
     7981 ctttgtttga actcatcacg aatatcctgc cgcgacattc gcagcttttt caggtggctg
     8041 aagatttgga aaaagacgtc aaatcccacc attggaatga caccaagcac caccagcagt
     8101 gcgcatagcc ctaccaaatc catcgcatta cccatggcgg taatcggaga ctcggccagt
     8161 aagcgcatca tctgcggcca gtgatgccag agaaaaaacc ccgtcacgct gccaaccaag
     8221 atggttttca aaattgcttt aagcaactcc gcgccagtct gtgccgagaa catccgttta
     8281 atgcccggta gcgggttgag tttggaaaac ttcggctgca aggattttcc gctaaatacc
     8341 agccccccca gcatgaccgg agaaatgagc gctaccagca ccacgccgct aatcagcggt
     8401 agcagcgcca gcatggcttc tctgatcagc agaataatct gcccgaggat cagattcggg
     8461 tctttgataa tactgtgatc aaaatgcagc ccagcggaga gcatgcccga caatcgacgg
     8521 gccagcgaca caccgccgaa ccagataaca cacacgccca ccagcaaaat cagcagtgag
     8581 gtcagttcac gggaacgcgg gatttagggc ttttgctgcc acacatcaag catagtgtgc
     8641 gtttgtcgga tgcggcgtca tcgccttatc agaccgcctg atatgacgtg gtcacgccac
     8701 atcaggcaat acaaatcaaa atccaagact atccaacaaa tcgtccacct gatcctgact
     8761 ggctaccaca ccggctttgc tggtatcgac ctgaggtcca ttaagcaaac tctggttttc
     8821 acgttttgga cgcgactcct gttccgggat gttttccaac agcaccatca gcaactggcg
     8881 ttcgatctcc tgaatgacat ccatcatccg cttaataacc tgcccggtga gatcctgaaa
     8941 atcctgcgcc atcatgattt ccagcagttg cgcgttagta aagctggtat gcgcgggtac
     9001 atctgccaga aattgtcgtg tatctgttac cagttcacgg gcgtcggcaa ggtcaatcgg
     9061 atcggcaaac cagtcatccc aacgttgggt taacgctttt gctgatttct ccatttgatc
     9121 ctgatgcggt tgtgacgcct caacactgtt cagcgcccgc tccgcagcct gggcggtcat
     9181 ctgcacaaca tagtacaaac gatcgcgcgc atcggggatg gcttccgccg cttcggcaat
     9241 ggcctgatcc agccccagtt cccgcaaact gtcgcgcagc atacgcgtca ggctgccgat
     9301 gcgcgcaatg atatcgccag ctgaatgctc gtcagcaggt ttgattgatg gttgcatcat
     9361 agtcgcatcc tcacatgtcc agtttatcaa agattttgtt gagtttttcc tccagcgtcg
     9421 cggcggtaaa tggcttcacc acatagccac tggcccccgc ttgcgccgca gcaatgatgt
     9481 tctctttctt cgcttctgca gtcaccatta acactggcaa tgccgacatc gcgccatccg
     9541 cacgaattgt tttcagcaat tccaggccat ccatattggg catgttccag tcggagataa
     9601 caaatccata accgcctgcc tgcaacttat tgagagcgtc gacgccatct tccgcttcct
     9661 caacattatt gaatcccagc tctttcagca ggttacgcac tatgcatcgc atggtggaaa
     9721 agtcatccac aaccaaaaat ttaagttctt tatccgccat ttcacactcc tgatttaaat
     9781 acgtatcgcc tgtccggcac taatttttgc caacatttgc tggcttacct ggctaagatc
     9841 gaccacttcg cagacaccac ccatattgat ggcctcgcgc ggcatgccga acaccacgca
     9901 acttgcttcg ttttgcgcaa gggtccatgc ccccgcctga cgcatcgcca acattcccac
     9961 cgggccgtcg tgaattttga tttgataatt tgcgccacta cgcgccagtt ccatatgccg
    10021 atcgcccggc gcaatatagg catgccccgg caagacacgt tctccgtctt cggcttcttt
    10081 aaccccgatc tggcaaagct tattaagtct gtcggcaaaa gagcgggtga aaccgggcgg
    10141 catatgttgg gtaattaaca atgccgggct ggaaagcggc aacggttgca gtacgtgacg
    10201 aattgcctca gttccacccg ttgaagcacc aatcgcaatc agtttttcag aactcaacaa
    10261 cggacccgcc ttcagcgttg tcggtgctga caatggctta tgtgctgcaa ggctcgcctt
    10321 tgctgccgta cgcacctttt cagcaatcat ttcgctatac gccagcattc cttcgcgaat
    10381 acccagttgc ggtttggtga caaaatctat tgcccccagc tccagcgcgc gcagcgtgac
    10441 ttctgatcct ttgccggtca aggaagaaac catcacaacg ggcattggac gcaaacgcat
    10501 taatttttcg aggaaatcca gtccgtccat ccgcggcatt tcaacatcca gcgtcagcac
    10561 atcgggattg aatttcttaa tcaagtcacg cgcgaccagc ggatcaggcg cggtcgccac
    10621 catttccatg tcgctatggc tgttgatgat ttctgtcatg atctggcgca tcagtgccga
    10681 atcatcgaca gataacaccc tgattttgct catcgttaat ccttacttag cgcatacacc
    10741 gtctgaccac gcagcgtgaa gcggcgctca aggtggctaa agttttcaga gtgacccgca
    10801 aacagcaatc cgtcgggttt aaggagcgga acaaagcggc gcaaaatctc ctgctgggta
    10861 ttttgatcga agtagatcat gacgttacga cagaagatcg catcaaacgg ccccggcacg
    10921 gtgtactgtt tcgccagtag attcagcggg gcaaaatcaa catagttcgc cagctcctga
    10981 cgcacgcgta ccagcccttc atgcggtccc gtccctcgca tgaaataccg ttgcagttgc
    11041 tgcggcgtca ggtttttcaa ctcttcatgg cgataaatac cgcttctggc tttttccagc
    11101 acttcggtgt cgatatcact ggcaaacact ttccagcgtc cgggcgcggt gcccaatgtg
    11161 tcagccagcg tcatcgcaat gctgtacggc tcttcgccgg ttgaagccgc cgcgctccat
    11221 acgcgatact cgccagaacg gcgacgtgcg tgatccgcga gcagagggaa atgatgtgcc
    11281 tcacggaaaa atgccgtcag attcgtggtc agcgaattga taaacgcctg ccactcaccg
    11341 ctgtgctgat tagattccag caagttcaga taatgaccga aatccgtcag tcccagcgaa
    11401 cgcaaacgac gaaccagtcg gttgtaaacc atgtcgcgtt tatgatcggc caggacgatc
    11461 ccggctcgtt gatagatcaa ttgacttatc cgccgaaaac tgtaccgaga acaaccaggt
    11521 aacctaataa gataagcacg acatcatcct tccactgttg accatgacag gatgttcagt
    11581 cgtcaggcgt taacgcgcga ttggggcaaa aaaaagcagc ggtacgtcgt taccgctgct
    11641 ggcatgttgc gccacaccgt atcagttaaa cagcctgtac tctctgttca tccagcagtt
    11701 gtgggataat atcggcagga ttctgggaaa gtttacgtct ttttactgcc cgggatggcg
    11761 gttgacataa gctgcaggca aagctgccaa caggctggtg agcgtgggta ataaaattgc
    11821 cgccgcagca gttgcagctg gaaagttgca gtaatccact ttcaacaaac cgccccaatg
    11881 tccaggcacg ggttaatgcc agcagtggtc cttcttctgc ttgtgggcac tgttcaaggt
    11941 ataaacggta ggctttgatc accgcatcga cgccattaca caaacctgtt ttcagtaaaa
    12001 actgccatgc attacagaac atcgaagcat gaacgttttg ttcccaggtc ataaaccagt
    12061 cggttgagaa tggcagcatg cctttcggcg gtgggcttcc gcgcagttct ttataaagtt
    12121 ttatcaggcg tccgcgactt aactgtgttt cgctttccag catctgcaaa cgagcgccca
    12181 gggtgatcaa ttcaatatcc cgcgcttcct gaacaatgct tttttcactc atgatcaggc
    12241 ccttttcttg cgcagcgctt cttcaggctg attaacatca ttcagcaagc gtgttgagag
    12301 catgatgccg gtatgaattt gctggagatc gttaacgcgg gaatcttgcg tcaactgagt
    12361 aatcgtctgg tggctgtcaa aacggaagtg acaaaccagt tgattggttt ctgccagctt
    12421 aaccatttgc ggaagagtca gtgccgctaa cgttgtcgcc atttcttcat ttatgccgag
    12481 acgaaacata gcggacgctt tgtcctgaac aatcaaacgc tgtgcaagta gtaaatatga
    12541 caagttgatg tcataaatgt gtttcagcaa ctcggaggta tgcattattc ccacccagaa
    12601 taaccaactt tatttttatg cggtttcacc gcaccccgtg atgtcgccgg gaagccccgg
    12661 taaaaaataa ttagcattag aatagtgcga taagctgcaa taagcagaac cacctttttg
    12721 gtttaatatg tccttacaaa tagaaatggg tctttacact tatctaagat ttttcctaaa
    12781 tcgacgcaac tgtactcgtc actacacgca catacaacgg aggggggctg cgattttcaa
    12841 taatgtgtga tgcagatcac acaaaacact caattactta acataaattt ataagccata
    12901 cttttgtttt gggtgtattt caatctgtta aaaagttttt cgctacgcta gcaagcaaaa
    12961 atgaaactgg aataatcgaa atgggatgtt gcgcacagtt aaaataactc accgtaaata
    13021 atcatctgct ataaataatc actttcatgc aataccagat aagctatttt taaacagaca
    13081 cttaccgcac aacaaactaa ttaaatacaa agaa
//
LOCUS       DAZPEB010000114        13097 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZPEB010000114 DAZPEB010000000
VERSION     DAZPEB010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13097
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.115"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            176..1150
                     /gene="cysB"
                     /locus_tag="ACHURJ_002756"
     CDS             176..1150
                     /gene="cysB"
                     /locus_tag="ACHURJ_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFR1281639.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            1469..1609
                     /locus_tag="ACHURJ_002757"
     CDS             1469..1609
                     /locus_tag="ACHURJ_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFR1281640.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
     gene            1612..1779
                     /gene="yciX"
                     /locus_tag="ACHURJ_002758"
     CDS             1612..1779
                     /gene="yciX"
                     /locus_tag="ACHURJ_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFR1281641.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            1893..1988
                     /gene="ymiC"
                     /locus_tag="ACHURJ_002759"
     CDS             1893..1988
                     /gene="ymiC"
                     /locus_tag="ACHURJ_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFR1281642.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            2152..4827
                     /gene="acnA"
                     /locus_tag="ACHURJ_002760"
                     /pseudo
     CDS             2152..4827
                     /gene="acnA"
                     /locus_tag="ACHURJ_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
     gene            complement(4891..5481)
                     /gene="ribA"
                     /locus_tag="ACHURJ_002761"
     CDS             complement(4891..5481)
                     /gene="ribA"
                     /locus_tag="ACHURJ_002761"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFR1281643.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            5651..6415
                     /gene="pgpB"
                     /locus_tag="ACHURJ_002762"
     CDS             5651..6415
                     /gene="pgpB"
                     /locus_tag="ACHURJ_002762"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFR1281644.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLLWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            6564..6872
                     /gene="lapA"
                     /locus_tag="ACHURJ_002763"
     CDS             6564..6872
                     /gene="lapA"
                     /locus_tag="ACHURJ_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFR1281645.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            6879..8048
                     /gene="lapB"
                     /locus_tag="ACHURJ_002764"
     CDS             6879..8048
                     /gene="lapB"
                     /locus_tag="ACHURJ_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFR1281646.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNRARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            8241..8978
                     /gene="pyrF"
                     /locus_tag="ACHURJ_002765"
     CDS             8241..8978
                     /gene="pyrF"
                     /locus_tag="ACHURJ_002765"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFR1281647.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSDAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            8978..9304
                     /gene="yciH"
                     /locus_tag="ACHURJ_002766"
     CDS             8978..9304
                     /gene="yciH"
                     /locus_tag="ACHURJ_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFR1281648.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(9430..9648)
                     /gene="osmB"
                     /locus_tag="ACHURJ_002767"
     CDS             complement(9430..9648)
                     /gene="osmB"
                     /locus_tag="ACHURJ_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFR1281649.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(9917..10666)
                     /locus_tag="ACHURJ_002768"
     CDS             complement(9917..10666)
                     /locus_tag="ACHURJ_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFR1281650.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEVIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            complement(10756..10929)
                     /gene="yciZ"
                     /locus_tag="ACHURJ_002769"
     CDS             complement(10756..10929)
                     /gene="yciZ"
                     /locus_tag="ACHURJ_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFR1281651.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            10966..11094
                     /gene="ymiD"
                     /locus_tag="ACHURJ_002770"
     CDS             10966..11094
                     /gene="ymiD"
                     /locus_tag="ACHURJ_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFR1281652.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTGYSAKYIYSSTYARFR"
     gene            complement(11076..13061)
                     /gene="pdeR"
                     /locus_tag="ACHURJ_002771"
     CDS             complement(11076..13061)
                     /gene="pdeR"
                     /locus_tag="ACHURJ_002771"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFR1281653.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPDRTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTCKGQRLFLFRNKFVHSGSGKSEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQDLIDHAINHADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSTAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPRLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
BASE COUNT         3333 a         3085 c         3293 g         3386 t
ORIGIN      
        1 tcgcgaaagc ggcccttttt tattgcatat tattttttct tcacacctat acactaaggc
       61 tataaatgat atagtggtta tagttagcac cttttttatt attaaatcgt attagtcacc
      121 cgccaggtgt gacgaaaaaa cgatgttctg atggcgtcta agtggatggt ttaacatgaa
      181 attacaacaa cttcgctata ttgttgaggt ggtcaatcat aacctgaatg tctcatcaac
      241 agcggaagga ctttacacat cacaacccgg gatcagtaaa caagtcagaa tgctggaaga
      301 cgagctaggc attcaaattt tttcccgaag cggcaagcac ctgacgcagg taacgccagc
      361 agggcaagaa ataattcgta tcgctcgcga agtcctgtcg aaagtcgatg ccataaaatc
      421 ggttgccgga gagcacacct ggccggataa aggctcactg tatatcgcca ccacgcatac
      481 ccaggcacgc tacgcattac caaacgtcat caaaggcttt attgagcgtt atcctcgcgt
      541 ttctttgcat atgcaccagg gctcgccgac acaaattgct gatgccgtct ctaaaggcaa
      601 tgccgatttc gctatcgcca cagaagcgct gcatctgtat gaagatttag tgatgttacc
      661 gtgctaccac tggaatcggg ctattgtagt cactccggat cacccgctgg caggcaaaaa
      721 agccattacc attgaagaac tggcgcaata tccgttggtg acatatacct tcggctttac
      781 cggacgttca gaactggata ctgcctttaa ccgcgcaggg ttaacgccgc gtatcgtttt
      841 cacggcaacg gatgctgacg tcattaaaac ttacgtccgg ttagggctgg gggtaggggt
      901 cattgccagc atggcggtgg atccggtcgc cgatccggac cttgtgcgcg ttgatgctca
      961 cgatatcttc agccacagta caaccaaaat tggttttcgc cgtagtactt ttttgcgcag
     1021 ttatatgtat gatttcattc agcgttttgc accgcattta acgcgtgatg tcgttgatgc
     1081 ggctgtcgca ttgcgctcta atgaagaaat tgaggtcatg tttaaagata taaaactgcc
     1141 ggaaaaataa tttcttgcgt tattttcggc accttttatg tagcgaaggt gccggaatat
     1201 attctctttt gttacttatt tttataccgt gcacaatatt tttatccctc atatttatag
     1261 ggtaaattac ctgtaaagta gtgtctaatt acccgcctcg caaattccag ttttcaaata
     1321 tctatttcag gtcaaaagat tgaattattc actggagacg attcgtaaat tcgctggatt
     1381 tttgactaaa gtttctttag gatttgtctc atcgtatgat taattacacc aatcgttcaa
     1441 cgtcttgctg ataagtgatg gcgattatat gaggttagca atgccttctg gaaatcagga
     1501 accccgccgc gatcctgaat taaaacgtaa agcctggctg gcggtttttc ttggttctgc
     1561 acttttctgg gtggttgtcg cactgctgat ttggaaagtg tggggataac tatggtcggt
     1621 caggagcaac tggagtcgtc accattatgc cagcatagtg acaatgaaac agaaacgaaa
     1681 cgggaatgtt ccgtcgttat tccagacgac tggcaactaa catcgcagca gcaagccttt
     1741 atagaactgt ttgctgaaga tgatcagccg aaacaataat tatcatcatt cttattaccc
     1801 atttttaatg aattaaaggg cttttaatac accgcagcaa taacagcttg agttatctca
     1861 acacaaaata ataaccgtta agggtgtagc ctatgatcaa cacaaatatg aaatattggt
     1921 cctggatggg cgcgttttct ctgtcgatgc tcttctgggc cgaactcctc tggatcatta
     1981 ctcactgatc cttgaccccg ctgcggcggg gttgtcattt gctttgccac aaggtttctc
     2041 ctcttttatc aatttgggtt gttatcaaat cgttacgcga tgtttgtgtt atctttaata
     2101 ttcaccctga agagaatcag ggcttcgcaa ccctgtcatt aaggaggagc tatgtcgtca
     2161 accctacgag aagccagtaa ggacacgttg caggccaaag ataaaactta ccactactac
     2221 agcctgccgc ttgctgctaa atcactgggc gatatcaccc gtctacccaa gtcactcaaa
     2281 gttttgctcg aaaacctgct gcgctggcag gatggtaact cggttaccga agaggatatc
     2341 cacgcgctgg caggatggct gaaaaatgcc catgctgacc gtgaaattgc ctaccgcccg
     2401 gcaagggtgc tgatgtagga ctttaccggc gtacctgccg ttgttgatct ggcggcaatg
     2461 cgcgaagcgg ttaaacgcct cggcggcgat actgcaaagg ttaacccgct ctcaccggtc
     2521 gacctggtca ttgaccactc ggtgaccgtc gatcgttttg gtgatgatga ggcatttgaa
     2581 gaaaacgtac gcctggaaat ggagcgcaac cacgaacgtt atgtgttcct gaaatgggga
     2641 aagcaagcgt tcagtcggtt tagcgtcgtg ccgccaggca caggcatttg ccatcaggtt
     2701 aacctcgaat atctcggcaa agcagtgtgg agtgaattgc aggacggtga atggattgct
     2761 tatccggata cactcgttgg tactgactcg cacaccacta tgatcaacgg ccttggcgtg
     2821 ctggggtggg gcgtgggtgg gatcgaagca gaagccgcaa tgttaggcca gccggtttcc
     2881 atgcttatcc cggacgtagt gggctttaaa cttaccggta aattacgtga aggtattacc
     2941 gccacagacc tggttctcac tgttacccaa atgctgcgca aacatggcgt ggtggggaaa
     3001 ttcgtcgaat tttatggtga tggtctggat tcactaccgc tggcggatcg cgccaccatt
     3061 gccaatatgt cgccagaata tggtgccacc tgtggcttct tcccaatcga tgctgtaacc
     3121 ctcgattaca tgcgtttaag cgggcgcagc gaagatcagg ttgagttggt cgaaaaatat
     3181 gccaaagcgc agggcatgtg gcgtaacccg ggcgatgaac cgatttttac cagtacgtta
     3241 gaactggata tgaatgacgt tgaagcgagc ctggcagggc caaaacgccc acaggatcgc
     3301 gttgcactgc ccgatgtacc aaaagcattt gccgccagta acgaactgga agtgaatgcc
     3361 acgcataaag atcgccagcc ggtcgattat gttatgaacg gacatcagta tcagttacct
     3421 gatggcgctg tggtcattgc tgcgataacc tcgtgcacca acacctctaa cccaagtgtg
     3481 ctgatggccg caggcttgct ggcgaaaaaa gccgtaactc tgggcctcaa gcggcaaccg
     3541 tgggtcaaag cgtcgctggc accgggttcg aaagtcgttt ctgattatct ggcaaaagcg
     3601 aaactgacac cgtatctcga cgaactgggg tttaaccttg tgggatacgg ttgtaccacc
     3661 tgtattggta actctgggcc gctgcccgat cctatcgaaa cggcaatcaa aaaaggcgat
     3721 ttaaccgtcg gtgcggtgct gtccggcaac cgtaactttg aaggccgtat ccatccgctg
     3781 gttaaaacta actggctggc ctcgccgccg ctggtggttg cctatgcgct ggcgggaaat
     3841 atgaatatca acctggctgc tgagcctatt ggtcatgatc gtaaaggcga cccggtttat
     3901 ctgaaagata tctggccatc ggcacaggaa attgcccgtg cggtagaaca agtctccaca
     3961 gaaatgttcc gtaaagagta cgcagaagtt tttgaaggca cagcagagtg gaaggaaatt
     4021 aacgtcacac gatccgatac ctacggttgg caggaggatt caacctatat tcgcttatcg
     4081 cctttctttg atgaaatgca ggcaacacca gcacctgtgg aagatattca cggtgcgcgg
     4141 atcctcgcaa tgctggggga ttcagtcacc actgaccata tctctccggc gggcagtatt
     4201 aagcccgaca gcccggcggg tcgatatcta caaggtcggg gtgttgagcg aaaagacttt
     4261 aactcctacg gttcgcggcg tggtaaccat gaagtgatga tgcgcggcac cttcgccaat
     4321 attcgcatcc gtaatgaaat ggtgcctggc gttgaagggg ggatgacgcg gcatttacct
     4381 gacagcgatg ttgtctctat ctatgatgcc gcgatgcgct ataagcagga gcaaacgccg
     4441 ctggcggtga ttgccgggaa agagtatgga tcaggctcca gccgtgactg gacggcaaaa
     4501 ggtccgcgtc tgcttggtat tcgtgtggtg attgccgaat cgtttgaacg aattcaccgt
     4561 tcgaatttaa ttggcatggg catcttgccg ctggaatttc cgcaaggcgt gacgcgtaaa
     4621 acgttagggc taaccgggga agagaagatt gatattggcg acctgcaaaa tctacaaccc
     4681 ggcgcgacgg ttccggtgac gcttacgcgc gcggatggta gccaggaagt cgtaccctgc
     4741 cgttgtcgta tcgacaccgc gacggagttg acctactacc agaacgacgg cattttgcat
     4801 tatgtcattc gtaatatgct gaagtaacaa gtatttgctt gccggttatt ttgcttccgg
     4861 caagcaaatt aattccacaa tgcgagaggg ttatttgttc agcaaatgac ccattttctc
     4921 ggctttggta tcgagataat gttcgttatt ggggttacga cctacaatca atggtacgcg
     4981 ttcaacaata ttaatccctg cttcggtcag aatttcgact tttttcgggt tattggttaa
     5041 caagcggact tcattgacgc caaggagttt gaacatatca gcgcaaagag tgaagtcgcg
     5101 ctcatcagcg gcgaagccta actggtggtt agcctctacg gtatcgtaac cttgatcctg
     5161 cagtgcgtaa gcgcggattt tattcagcag accaatgtta cgaccttcct gacggtgata
     5221 cagcaaaata ccacggcctt cctcggcaat ttgcgtcaat gccgcttcga gctggaagcc
     5281 acaatcgcag cgcaagctga acagggcgtc accggtcaga cattcggaat ggacgcgcgc
     5341 aagtaccggg gtatgcccgg aaatatcgcc atagactagc gcgacatgat cgtgtccggt
     5401 tgccagttct tcaaatccca ccatcaggaa atcgccccat ggggttggca gtttggcttc
     5461 tgccacacgt ttaagctgca tgaaattctc cagataatgc tggttctgta ttggcttatt
     5521 ttgccataac gagaaggggt tcacctaatc acgacgcgtc gatcgttcac ggaatggcac
     5581 aaatctgtca atttttcctg gaactggcgt tttcagttat gattgtggga cttatcaaaa
     5641 aggagaggcc atgcgttcga ttgccagacg taccgcagtg ggagctgcac tattgcttgt
     5701 catgccagta gccgtatgga tttctggctg gcgttggcaa cctggagaac aaagttggct
     5761 actaaaagcg gctttttggg ttactgaaac tgtcacccag ccctggggcg tcattacaca
     5821 tttgatttta ttcggctggt ttctctggtg tctgcgtttt cgcattaagg ctgcctttgt
     5881 attatttgcc attctggcgg ccgcaatcct tgtgggacaa ggcgttaaat cctggatcaa
     5941 agacaaagtc caggaaccac gaccttttgt tatctggctg gaaaaaacac atcatattcc
     6001 ggttgatgag ttctacactt taaagcgagc agaacgcgga aatctagtga aagaacagtt
     6061 ggctgaagag aaaaatatcc cacaatattt gcgttcacac tggcagaaag agacggggtt
     6121 tgcctttcct tccggtcaca cgatgtttgc tgccagttgg gcactgctgg ccgttggttt
     6181 gctgtggccg cgtcggcgaa cgttaaccat tgctatcttg ctgctctggg caacgggagt
     6241 catgggaagc cgcctgctgc tcgggatgca ttggccacgc gatctggtag tagctacatt
     6301 gatttcgtgg gcgctggtgg cggtggcaac gtggcttgcg caacgaattt gtgggccatt
     6361 aacaccacct gcggaagaaa atcgcgaaat agcgcaacga gaacaagaaa gttaacgctg
     6421 gttgattttc cgaatttagc ccttaaatca tcaacaatgc gcgtggatgc catttcgcag
     6481 acggcgcgaa aatggtactt taaagggcta ttgcggtaag ttgaccataa tttattcgct
     6541 ctaaccacat aacgggaagt aatgtgaaat atttactcat tttcttactg gtgttagcga
     6601 tcttcgtgat ttcggtcacg ttgggtgcgc agaacgatca acaggtgacg tttaattatc
     6661 tgttagcgca aggggagtac cgtatttcca cattgctggc ggtattgttt gctgcggggt
     6721 ttgctatcgg ttggttgatt tgtggcctgt tctggctgcg agttcgtgtt tccctggcgc
     6781 gcgctgaacg taaaataaag cgactggaaa accagctttc acccgcgact gacgtggctg
     6841 tagtgccgca ctcgtcagcg gcgaaggaat aactttctat gctggagttg ttgtttctgc
     6901 ttttgcctgt agccgctgcc tatggctggt atatgggccg cagaagtgcg caacaaaaca
     6961 agcaagatga agccaaccgc ttgtcgcgtg attacgtagc gggggttaac ttcctgctta
     7021 gtaatcaaca ggataaagcg gtagacctgt ttctcgatat gcttaaagag gatacaggca
     7081 ccgttgaagc ccaccttacg ctcggaaacc tgttccgttc gcgtggcgaa gttgatcgcg
     7141 ctattcgcat ccatcagacc ctaatggaaa gcgcctcgct gacctatgaa cagcgtctgt
     7201 tggcgattca acaactaggg cgtgattaca tggccgccgg gttatacgac cgcgcggaag
     7261 acatgttcaa tcagctgacc gatgaaactg acttccgcat tggcgctctg caacagttgc
     7321 tacaaatcta ccaggctacc agtgagtggc agaaagcaat tgatgttgcc gaacgcctgg
     7381 tgaagctggg taaagataaa cagcgcgtcg aaattgccca tttctactgt gagttagccc
     7441 tgcagcatat ggccagcgac gatctcgatc gtgccatgac cttgctaaaa aaaggggcgg
     7501 cggcagataa aaacagagcc cgcgtatcca tcatgatggg acgcgtgttt atggcgaaag
     7561 gagaatacgc caaagccgtc gaaagtttgc aacgcgtgat atcccaggac agagaactgg
     7621 tcagcgaaac gctggaaatg ttgcaaacct gctatcagca gttgggtaaa actgccgaat
     7681 gggcagaatt cctgcaacgc gcggtggaag agaacaccgg tgccgatgct gaattgatgc
     7741 ttgctgatat catcgaagcg cgcgacggta gtgaggccgc acaggtctat attacgcgcc
     7801 agcttcagcg tcatccgacc atgcgtgtgt tccataagtt aatggattac cacttaaatg
     7861 aagcggaaga agggcgtgcc aaagagagcc tgatggtgct gcgtgacatg gttggcgaaa
     7921 aggtacgtag taagcctcgt tatcgctgcc agaaatgtgg ttttaccgca tacaccctct
     7981 actggcattg tccgtcttgt cgggcctggt caaccattaa accgattcgc ggtcttgatg
     8041 gcctgtaatt tttaaaaaaa tccgacttta gttacaacat gctagttatt gatgttccat
     8101 tgtgctccgg caacgacgga gcagaaaaag cctgccaggg gaaaaatcgc aactgttaat
     8161 tttttatttc cacgggtaga atgctcgccg tttacctgtt tcgcgccact tccggtgccc
     8221 atcatcaaga aggtctggtc atgacgttaa ctgcttcatc ttcttcccgc gctgttacga
     8281 attctcctgt ggttgttgcc cttgattatc ataatcgtga tgacgcgctg tcctttgtcg
     8341 acaagatcga cccacgcgat tgtcgtctga aggtcggcaa agagatgttt acattgtttg
     8401 ggccacagtt tgtgcgcgaa cttcaacagc gtggttttga tatctttctt gacctgaaat
     8461 tccacgatat tcctaacact gcagcgcacg ctgtcgctgc tgcagctgac ttaggcgtgt
     8521 ggatggtgaa tgttcatgcc tctggtgggg cgcgtatgat gaccgcagcg cgtgaggcac
     8581 tggttccgtt tggcaaagat gcaccgcttt tgattgctgt aacagtgttg accagcatgg
     8641 aagctagcga cctggccgat cttggcgtga cactgtcacc tgcagattat gcagaacgtc
     8701 tggcggcact gacgcaaaaa tgtggccttg atggtgtggt gtgttctgct caggaagctg
     8761 tgcgctttaa acaggtattc ggtcaggagt tcaaactggt tacaccgggc attcgtccgc
     8821 aggggagtga tgctggtgac cagcgccgca ttatgacgcc agaacaggcg ttggcggctg
     8881 gtgttgatta tatggtgatt ggtcgcccgg taacgcaatc ggtagatcca gcgcaaacac
     8941 tgaaagcgat caacgcctct ttacagcgga gtgcatgatg agtgattcca acagccgtct
     9001 ggtctactca acggaaaccg gacgtattga tgagccaaaa gcggccccag tacgccctaa
     9061 aggcgatggt gtggtgcgta ttcagcgtca gaccagtgga cgtaaaggta agggcgtttg
     9121 cctgattacc ggtatcgatc tcgatgatgc cgaactgaca aaacttgcag cggaactgaa
     9181 gaaaaaatgc ggctgcggcg gagcagttaa agatggagtt attgaaatcc agggcgataa
     9241 gcgtgattta ttaaaatcac tacttgaagc gaaagggatg aaggtaaaac tcgcaggcgg
     9301 ttaacataaa aagccacgga tatatccgtg gctttcgaat attttactgt gtgtattatt
     9361 cagttatttc gtactgaaca aaatcaagcg tattataatt gcgagtcgat aatcgacatt
     9421 tatttacgat tatttaccga cctggtgacc aataacacca ccgacagctg caccacctaa
     9481 tgtacccaac gtactgccat cggtcagtac tgcaccgcct aatgcccctg cacccgcgcc
     9541 gattgcggtg ttgcggtccc gtttagacca gttagaacag gcactcagag acattgccaa
     9601 agtaattgcc agaacagccg cggtcatttt tttgctcgtt acaaacataa tactctctcc
     9661 tgaatttatg attcacggaa gtaagctctc tataactata atagctaaga ataagcctgg
     9721 tgaaattatt ccgtgaaatc tgctcgcgcg cagggttata tcacgcaggt gataatgact
     9781 tcctgttata tcgctgataa taattttata tcttaagcgt gttaataaca ggtaaatagt
     9841 cttaattatc aaccaggaat catcttagag cggatgattt gccaaactgc aaatcatccg
     9901 tagagaaggg aaatggttaa atgtcaacga cgtgaacggt gagtttactg tgctcaagat
     9961 gcatcagatc gctggcgcgt attttcgaat cggtaatcac gcgattgaat cgctcaacgg
    10021 gaccgatgga gtatgaatgt acagcaccaa atttcgagct gtcagtcagg actatcactt
    10081 cgcactcttt ttccagcacg gcattgacca catcggtacg catcatatcg cgaccggtga
    10141 atccagtttc aggttgccag ccatcaatac caataaatgc tttgctgaaa tgcacctgct
    10201 ggatgcactg gcgtgtcaac gggccaacca tactttcgct ttttttctgg tacacgccac
    10261 cgagcaaaat gacttcacaa ggcgcgtctt tcagcaaatg cgcgatgtag ctgctgaccg
    10321 tgatgatagt gacatttttc ttctgctccc ccagagtccg ggcgagcagg gcattgctgc
    10381 tgccattttc gataaagatg gtttcgcccg gttgaaccag tgacgcggca aactcggcga
    10441 gttcacgctt cagcgtatag ttgctcatca tacgggtttc gacgtcatca ctatcaagcg
    10501 aaactgcaaa gccatgtgca cggcggaggt aactcagttt ttcgagggtg ttgagatcct
    10561 ggcgaatggt aacttcagaa actccagtgg cttttgccag atcggttacg ctaacctgac
    10621 cctggtcaat gaccatctgt agaatagttt gttgtcggga attcatcgct gtaattctta
    10681 taacgttata atactaaata aaaaaatact gacgcgccgg gcatcctcaa ataggaaaac
    10741 ctgaaggcac gattgttaaa tctcccacgg tgccttcggg atgtctggcg tagattcagc
    10801 aacctggcgc aagagttctg ctttaagtat ctcgagatta tggatcgcag agtggtaatc
    10861 gcctgccaca aggatatcta acacaatatc aatacgttcg gcaacgcgct gggcatcaaa
    10921 ttcagacatc aaggacatcc ttatagcaaa agtggaaaat gaataatgca aaaattgccg
    10981 ctaaaagaga agtgtttaac agcaacggct aattatcatc caggaatacg atatataatg
    11041 acgggatata gcgctaagta tatatattca tctacttatg cgcgcttcag atagcgttta
    11101 taccagcgtt cgaaggcgac ggcgggcatc ggtttggcaa acaaaaatcc ttgccgctca
    11161 ttgatcccgt tcttggttaa aaaagcatct tcctttgcac tctctacacc ttcggcgatc
    11221 acctgaagat tcaatgcctg ggccacagcg acgatcgccc ggaccagtga ctgcgagaca
    11281 ggttgtttgt gaatatctcg aacaaaaacc tggtcaagtt tgatggcatc gatcggaaag
    11341 cgcgccagtt gcgaaagtga agagtagccg gtaccaaaat catccagatg cacttgcgca
    11401 cctagttggc taaattgttg aataacagac agtgccagtt catcattctc aatcagacaa
    11461 ctctctgtca gttcaacatc tatagggcag tattcaaaat tgagttcctg gagaacctgt
    11521 ttcagggcgg tgaaaatggt ttgatcggcg agctgacgtg cagaaatatt taccgccact
    11581 cgcaggttta tgcctttatc ccgccacttt gccacctggc gtacgacatc gagaatcacc
    11641 caacggccta aaggcacaat tagccctgac tcttcggcgt aggaaatgaa gtccaaccgt
    11701 ggaatcaacc cacgttcagg tgactgccaa cgtactagtg cttccagact gcgcacttcg
    11761 ccacgccagg tgattttcgg ttgatagtga ataaccaact gatcgttttc cagtgcttta
    11821 cgcaagttgg tatccagcca gagatattca aatacccgtt gattcatttc tggggtaaaa
    11881 acgcaaaatt gtcctcgtcc gccttccttc gctgtgtaca ttgctgtgtc ggcgtgacga
    11941 ataatagccg tgctgtctga accatgttcg ggagagagtg caatacctac tgaacagctg
    12001 gtataaactt caattaaacc aatgcgaaag gggagccgta agcgggtcaa aattcgtgat
    12061 gccattgctt ccagcgcgct ttgtgaggtg ttggatgcca gtaccagaaa ctcatcccca
    12121 cctggacgcg ccaacacctg gtcatgttcg agacagctta aaatagccaa tgacacgtcg
    12181 cgtaataact ggtcaccaaa caaatgccca taggcgtcgt tgaccttttt gaaattatcc
    12241 aaatcaagat acacaacccc aactttattg ttatctgcat gattaatagc gtgatcgatt
    12301 aaatcctgca ttgcgttacg attcggcagt ccggtgatac tgtcggtatt tgccagaata
    12361 cgcagtcgct cctgagcgcg gcgctcttcg gtaatgtcgg tgccggaaca gattaaaaaa
    12421 atctcgcttt tgccactgcc gctgtggaca aatttattgc gaaacagaaa cagccgctgg
    12481 cctttacatg ttggtatcca cagttcgact tcatatgcat tgccgcttcg aaaaaataca
    12541 cggttattgc gcctggatgc cgcagcttca cgacggctca taaacagttt aaacacgctt
    12601 tggccaatga cgtcgtgttc tttcaaccca gtgtaatctt cacataaccg attgaagcgt
    12661 tggatattcc cccggctgtc gagaatcact attgcggaat gtgcttcaga aactacctgc
    12721 tcagcaaagg aaagcccgtg tgataaatca cgagcaactg ccggtgtgtc atgccatgca
    12781 gacgcgttgc cagcccattc ccgtttattg atttttcgtc ctaccagatg cacagaaaga
    12841 tcgctctcat cgacggtcag actcatcatc aggctggagg tgatgaccgt catttccctg
    12901 atgcgagcag cctgttcggc agataactgc aaagtacgat caggttctgt ggtttcggtg
    12961 gtagaaaagc gcaaaacatc gctgctttcc gtcaaccgcc agtatggatt gtgcgatccg
    13021 agaaagttgt acaacgttgt ggactcccta acggttttca tgatgcatat cctccggtta
    13081 aataaaagga acaacat
//
LOCUS       DAZPEB010000115        13094 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZPEB010000115 DAZPEB010000000
VERSION     DAZPEB010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13094
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.116"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..1632
                     /locus_tag="ACHURJ_002772"
     CDS             <1..1632
                     /locus_tag="ACHURJ_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFR1281654.1"
                     /translation="WMFTAIIAVCILFIIGIIFARLYRRASAEQAFVRTGLGGQKVVM
                     SGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFFVRVKPSVEGI
                     ATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENFVQGVQNTVAE
                     DLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLIQETERRRRERNEVEQDVE
                     VAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEAERRREAEQTR
                     ILAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKSIAIAAKSEQQ
                     SQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETKAVELTVRAKA
                     EKEAAEMQAAAIVKLAEATRKKGLAEAEAQRALNDAINVLSDEQTSLKFKLALLQALP
                     AVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQALSAALSYRTQA
                     PLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            1871..1930
                     /locus_tag="ACHURJ_002773"
     CDS             1871..1930
                     /locus_tag="ACHURJ_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR1281655.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            2247..2306
                     /gene="ibsE"
                     /locus_tag="ACHURJ_002774"
     CDS             2247..2306
                     /gene="ibsE"
                     /locus_tag="ACHURJ_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HFR1281656.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(2426..3859)
                     /gene="hldE"
                     /locus_tag="ACHURJ_002775"
     CDS             complement(2426..3859)
                     /gene="hldE"
                     /locus_tag="ACHURJ_002775"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFR1281657.1"
                     /translation="MKVTLSEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(3907..6747)
                     /gene="glnE"
                     /locus_tag="ACHURJ_002776"
     CDS             complement(3907..6747)
                     /gene="glnE"
                     /locus_tag="ACHURJ_002776"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFR1281658.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEKSLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQSPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFN
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMMLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(6770..8071)
                     /gene="ygiF"
                     /locus_tag="ACHURJ_002777"
     CDS             complement(6770..8071)
                     /gene="ygiF"
                     /locus_tag="ACHURJ_002777"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFR1281659.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDDSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTKTAMAKLALTEWLVSKAWQPFLDAKAQSKM
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            8313..8933
                     /locus_tag="ACHURJ_002778"
     CDS             8313..8933
                     /locus_tag="ACHURJ_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFR1281660.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPTRKRKERWMN"
     gene            8997..10235
                     /gene="cca"
                     /locus_tag="ACHURJ_002779"
     CDS             8997..10235
                     /gene="cca"
                     /locus_tag="ACHURJ_002779"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFR1281661.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(10392..11213)
                     /gene="bacA"
                     /locus_tag="ACHURJ_002780"
     CDS             complement(10392..11213)
                     /gene="bacA"
                     /locus_tag="ACHURJ_002780"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFR1281662.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(11304..11672)
                     /gene="folB"
                     /locus_tag="ACHURJ_002781"
     CDS             complement(11304..11672)
                     /gene="folB"
                     /locus_tag="ACHURJ_002781"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFR1281663.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            11777..12394
                     /gene="plsY"
                     /locus_tag="ACHURJ_002782"
     CDS             11777..12394
                     /gene="plsY"
                     /locus_tag="ACHURJ_002782"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFR1281664.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(12407..>13094)
                     /gene="ttdR"
                     /locus_tag="ACHURJ_002783"
                     /gene_synonym="dan"
     CDS             complement(12407..>13094)
                     /gene="ttdR"
                     /locus_tag="ACHURJ_002783"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HFR1281665.1"
                     /translation="LVDDVTQIKTRPEGMIRIGCSFGFGRSHIAPAITELMRNYPELQ
                     VHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAHLLTKNKRILCAAPEYLQKYPQPQS
                     LQELSRHDCLVTKERDMTHGIWELGNGQEKKSVKVSGHLSSNSGEIVLQWALEGKGIM
                     LRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYREPLYRSMKLRVCVEFLAAWCQQRL
                     GKPDEGYQVM"
BASE COUNT         3163 a         3682 c         3283 g         2966 t
ORIGIN      
        1 tggatgttta ccgcgattat tgccgtatgc attctgttta ttattggaat tattttcgcc
       61 aggctctatc gtcgcgcttc ggcagagcaa gcttttgttc gtactggttt aggtgggcaa
      121 aaagtggtga tgagcggtgg cgcaatcgtg atgccgatct ttcatgaaat aatccccatc
      181 aatatgaata ctctgaagct ggaagtcagc cgctcaacca ttgatagcct gattacgaaa
      241 gatcgtatgc gtgtcgatgt agtagtcgct ttctttgtac gggtaaaacc gtctgtagaa
      301 ggcatcgcca ctgcggcgca gacgttgggg caacgcacgc tatcaccaga agacttacgt
      361 atgttggttg aagataaatt tgtcgatgcc ctccgtgcaa cagctgcaca aatgaccatg
      421 catgagttac aggatacccg cgagaacttc gtgcagggag tacaaaatac tgtcgcagaa
      481 gacctgtcga aaaacggtct tgagctggaa agcgtttcac ttaccaactt taaccagacc
      541 tcgaaagaac atttcaatcc gaacaatgcc tttgacgccg aaggtttaac caaactgatt
      601 caggagacgg agcgccgtcg tcgcgaacgt aacgaagttg aacaggatgt agaagttgcg
      661 gtgcgtgaga aaaaccgtga tgcactttcg cgcaagttgg agattgaaca acaagaagcg
      721 tttatgacgc ttgagcagga gcagcaggtt aaaacccgta ctgccgaaca gaatgcacgt
      781 attgcggctt ttgaagctga acgtcgtcgt gaagcagagc agacgcgaat tctggctgaa
      841 cgacaaattc aggaaacgga gatcgagcgc gagcaggccg tccgctcaag aaaggttgag
      901 gctgaacgtg aagttcgtat taaagagatc gaacagcagc aggtcaccga aatcgccaac
      961 cagacgaaat cgatcgctat tgccgccaaa tcggaacaac agtcccaggc agaagcgcgt
     1021 gctaaccttg cactcgcaga agcagtaagt gcgcaacaaa acgtagaaac cactcgacag
     1081 accgcagaag ccgatcgtgc taaacaagtt gccctaatcg ctgccgcgca ggatgcagaa
     1141 accaaagcgg ttgaactgac cgtgcgggcg aaagcagaaa aagaagccgc agagatgcag
     1201 gcggcggcta tcgttaagtt agccgaagct acacgtaaaa agggtctggc ggaagcagaa
     1261 gcacaacgtg cgctgaacga tgctatcaac gtactttctg atgaacaaac cagccttaaa
     1321 ttcaaactgg ccttgttgca ggcgctacct gcggtaatag aaaaatccgt tgagccgatg
     1381 aaatctattg acggtatcaa gattattcag gtcgatggtc tgaatcgtgg cagcgctgcg
     1441 ggtgatgcaa acacgggtaa tgtggggggc ggaaacctgg cggaacaagc attatcagcc
     1501 gctctctctt accgcacaca ggcaccgctg attgactcct tgctcaatga aattggcgtt
     1561 tcaggcggct cactggcggc attgacttca cccttaacct caacaactcc cgtcgccgaa
     1621 aacgtagaat aaattttcag ctatgaacgt ccctggcaca aaaagccggg gattttttat
     1681 atctgcgttc cgctaaaaac tgcaaatgat caggtcgttg caacgttttg cgtgaccgct
     1741 cggggaaggc aaaactgcct ctgggaaagc gttgcgcgag gtccggcgct catcaacaat
     1801 cggggagtca gcaaagggct gaaacgggaa agcccctccc gaggaagggg ccttacagaa
     1861 ggaaagggtt atgatgaagc tcgtcatcat actgattgtg ttgttactcg taagtttcgc
     1921 agcttattaa cagccaatca gaggggggag aaatcctccc tcacccttgt tcctttactt
     1981 taggttgaaa aaaaatcagc gtcaagaagg tttcattaac acatatccct agcccaaaaa
     2041 gccggggatt ttttatatct gcgttccgct aaaaggtgca aatgctcagg acgttgcagc
     2101 gttttgcgtg accgctcggg gaaggcaaaa ctgcttcagg gaaagcgttg cgcggggtcc
     2161 ggcgctcatc aacaatcggg gagtcagcaa ggggctgaaa cgggaaagcc cctcccgagg
     2221 aaggggcctt atagaaggaa agggttatga tgaagttcgt catcatactg gttgtgttgt
     2281 tactgttaag tttcccgact tactaacaac tgatcagagg ggggagaaat cctcccttac
     2341 ccttgttcct ttactttagg ttgaaaaaac aacagcgtca ataggcctgc ctgctacgaa
     2401 gcgagatctg tgaaccgctt tccggttagc cttttttatc ctgttggatc ttcttgatga
     2461 tgttagtggt cgagcaaccg tcttcgaagt tgagcaccag cacttcgccg ccgttggccc
     2521 agacttcttt actcccggcg atctcttctg gtttatagtc gccgcccttc accagtaaat
     2581 ctggcaggat cccggcgatc aagcgctgcg gcgtgtcttc ttcaaacgac accacccagt
     2641 cgaccgcttc cagtgcgccc agcacaatca tacgctgttc gagcgggtta acggggcggg
     2701 aatccccttt cagccgtttg gtggaggcat cgctgttcac agcgacaatc agacggtcac
     2761 ccagtttgcg agcatttgcc agataggaga cgtgcccggc atgcaggatg tcaaagacac
     2821 cgttggtcat taccaccttt tcgccgcgtt tacgcgctgc cgctacggcc agcttcagtt
     2881 cctcttcggt catcacgcca aagccggtat ctgcgcgccc acgcactgca ttttccagct
     2941 cgatcggcga aaccgtggag gttcccagtt tgccgaccac cacgccagcc gccgcattgg
     3001 caaagaagca ggcttcttcc agcgaattac ccgctgccag cgttgccgcc aggacgccaa
     3061 tcaccgtgtc gcccgcaccg gtaacgtcat acacttcctg cgcctgggtt ggcatatgca
     3121 gcggcgcttt acccggttgc agcagcgaca taccctgttc ggaacgggtc actaacagag
     3181 ccgagagttc gtaatcggca atcagtttca tgccgcgctc aacaatctct tcttcggtct
     3241 tacatttacc gacaacagct tcaaattccg agagattcgg cgttaacagc gtagcgccgc
     3301 ggtagcgctc aaaatcggta ccttttggat caatcagcac cggaacgccc gctttacgcg
     3361 ccagttggat catctgctgt acgcttgcca gcgcaccttt ggcgtagtca gaaagcacca
     3421 gcgcgccaat cgaactcagc gcctgattaa tccgctcgtg cagcggctgc ggatcaacgc
     3481 cttcgaaacc ttcttcgaaa tccagacgga tcagctgttg gttgcgggaa agtacccgta
     3541 atttggtgat ggtcggatgc gtcggtacag aaacgaagtc gcatttgacg ttgacgtcgg
     3601 ccagagattt actcagcgcg cgcgctgcat cgtcaatgcc cgtcaacccg accaggcgtg
     3661 cattagcacc gagagaagcg atattcatcg ccacgttagc cgcgccgccc ggacgttctt
     3721 cgatggtatt cactttaacc acgggcaccg gcgcttccgg cgagatacgg ctggtgggac
     3781 cgtaccagta acgatccagc attacatcac caaccaccat cactcctgca cgttcaaact
     3841 ctgacagcgt tactttcatt cctgtctcct gagagattca aaatttgcgc gcgataatac
     3901 catacttcat tcttccacca gccacttctg ccagcttgcc cgcaccagat cacgctctgc
     3961 ggtgaagcaa tcctccgaca catggcccgg caattcctgt aatgccagat gatgaagttc
     4021 atcgcgcagc gtcgtgtaag cacgggtcag cgccatcgct tcctgctctt ccataatgtc
     4081 gttttgcgcc agcagctcca gaatacgcac gttgtctgac cagcgcgtta acttcggttt
     4141 ttcatgagcg tagcgcaaca ccagatattg ggtaataaat tcgatatcgg taattccgcc
     4201 ttcatcggct ttgatatcaa agcgattacg atgtttattg ccgagatgag cgcgcatttt
     4261 ctcgcgcatt tcccgcactt ccgtttgcag agttttacct tcacgcggca gcatcataat
     4321 ctcgcgacgc actgcgtcaa agtgcgcggt gagctgcgga tcgccgtaca ctacacgcgc
     4381 acgcaccagc gcctgatgct cccacgtcca ggcctcgttt ttctgataat cagcaaatgc
     4441 ttctgcggat gtcaccagca ttcccgccgc cccggacgga cgcagtcgtg catccacttc
     4501 atacaaaatg ccggaagagg ttcgcgtatt gaacagatgc ataatgcgtt gcgccagacg
     4561 cagataaaac tgtcgcccgt cgatttcccg ctcgccgtca gtcatcgcat ccattgggca
     4621 atcatggagg aagatcaggt caagatcgga actgtagcct aactcccagc cgcccagctt
     4681 gccgtagccg accaccgcaa aaccacggcc ttcgcgatcg ttgaggtgat ttggcttacc
     4741 gtagcgggcc accatttgaa cccacgcctg ctgaacgacg gcatctatca tagcctctgc
     4801 cagccaggtt aagtgatcgc tcactttcat caccggtagc gtaccagcga tatccgctgc
     4861 ggcgatgcgt aacagctgcg cctgtttgaa ctgacgcagc gcctcaagct gttgctcttc
     4921 gtcatcttcc ggcacgcgca gcaaatactg gcgcaactca tcgcggtagg catcggtcgc
     4981 cgtcggctgg taaagagtgt ttggatcgag caattcatcc agcaataatg gataacgcgc
     5041 cagctggctg gcaatcatcg gcgatgcggc acacagagaa atcaaatgtt taagcgccgc
     5101 agggaattca ctgagcaatt ctaaataggt ggtgcgggta acaatcccca ccagcaaggc
     5161 ggtaatgcgc gacagcgtaa cggcagcgtc ttcacgcgca cagacatcac ttagcagatg
     5221 tggcatcaga tggtcgagca cctgacgtcc tcgcggcccg atagtacgct tatccagctc
     5281 tttgcggaaa tcagcaatca gcgttaggac ctgtttgcga tcatcctcgc taagatgcgc
     5341 cagcactggc gtagtgtcat cttcctgcaa cgcatcctgc cacagctcac gccactgttc
     5401 cgacagcgac tcttcctgag tttcactttc gtcgtcgcca atcaattcat taaacacccg
     5461 gcgcacattg gtcatatgtg cggtcagcgc cccggtcagt tgcggccagt cagcaaagtc
     5521 catcgcccac gccagccgcg cacggttaag ctcatcagaa ggaagcgttt gggtttgttc
     5581 gtcgttaatg ctttgcagca ggttttccag acgccgcagg aacagatatg ccactcgcaa
     5641 ttgttcagca tcgttttcag aaagcagatg cagcgcggca atgacgctga gcgttggcag
     5701 taaagagcgc gactgcagcg acggttcgcg cccaccgcga atgagctgga acacctgaac
     5761 gataaattca atttcgcgaa tgccgcctgc gccgagtttg atattgtcgg tcaaaccacg
     5821 tcgacgcact tcacgggcaa tcatcccttt catgttgcgc agcgactgaa tcacgctgaa
     5881 atcgatgtaa cgacggaaaa caaacgggcg cagcatcgca cgcaactcgt tagcatagac
     5941 gccttcgcta tcgcccataa tccgcgcctt gaccatcgcg taacgctccc agtcgcgccc
     6001 ctgctcctgg taataatcct ccaacgcggc aaagctcagc accagcgggc cactttcgcc
     6061 aaacggacgc agccgcatat ccacgcgata gacgaagcca tcctgcgttg gttgatccaa
     6121 cactttaatc agccgctgcc ccatgcgggt aaaaaactgc gcgttatcca gttcccggcg
     6181 tccgccctgc gtacaaccat gttccggcca ggcaaaaatc agatcgatat ctgaggagaa
     6241 attcagttcc ccaccgccca gcttacccat gcctaaaatc agcagcggtt gcgcttcgcc
     6301 ctgcgcattg cacggcgttc cccactcgcg gcagcaagcg tcatacagcc agtcacgcgc
     6361 ggcgacaatc agcgtctccg ccagatggct gagctgctgc aatatgctct cttcagtaac
     6421 caaagccagc gtttgcgccc aggcgatgcg caccataatg cgccgccgga atagccgcag
     6481 ctcgcgcatt aacccggctt cgtcactcac attgctgagc gcctcctgca accatgccgc
     6541 gtaatgctgc cattcatcag cctgcggcga ttggctttcc agttccgtca gccactctgg
     6601 atgcgcaatc acgctgtcct gcacaaaatc actgaaagta agtactgact tcgcctgtgc
     6661 gctaagtgat ttctcggcta aaggctctgg cagccgctca acaacggtct gccagtactg
     6721 ctgtaacggt gaagagagcg gcttcataag cgattttatc cttgcctgat taacgttttc
     6781 cgctgtgcaa ccagaacggt tcctgattgt ttgcctcatt acggaaatgt tcaatttcaa
     6841 tgcgttgccc ggtcgcaata gcgtgacgca gcccctgcca gttctccagc caggcttgcg
     6901 cgacgacagg atcatagtaa cccgccagca acagtattga gtcaatatca cgcgtcagac
     6961 gtggcaactg gtcacggtag cgatcgccca acggctggca gaaaacgctt ttcagttcag
     7021 cggcatggcg ggaaagatgg atatcggcaa agcgtttgaa ggaatcgctc attttactct
     7081 gcgctttggc atctaaaaac ggctgccagg ctttgcttac cagccattcg gtcaacgcca
     7141 gcttcgccat tgccgttttg gtagagtaga ccgccgtcac ggcagaaacc gcagaagcga
     7201 tggtcgcttc gcattgagtc agcaaatcgc gtaagtgagt gctcgctttg cgcggcacaa
     7261 taccaccgaa cagcatcagt gtatgacgga ccagaccaat ggctgccagc acctgttctt
     7321 tcgccgcatc gttgccgcgt acccacagtt cttcatgata ctgccattgc gctaacgcca
     7381 gctcgagcgc cgcttccagc ccctgttcca catcggcttt tgccgcaaca tgcaaaatgg
     7441 tggtcggttt gatttcacgc gccggattgc cctgcgccag atgatagcca cgcgccgctt
     7501 tgctcaggct gccctggcgt aatccggttt gcgataccag ttggttcgcc agtttcagca
     7561 ccgcgcgcgt gtcgccgcta agcagttcca gttccagctc acagataggt tcagcaaatt
     7621 cccccgcttt cacctccccc tgatcgagag cgatttcaat tcggctgtca tcgaccgcca
     7681 ccagccattt ttcgcgataa aaatcggtgc tgaacagcgg ttgtacgcgg gaagcgagat
     7741 cggcgggcaa ttcgccgttc ggccagactt ccgtcggtaa ctgcgccagg tcgagcgtcg
     7801 gttcgctcaa cgccacgtta tattccgggc gctggtgtaa gccgcctgtc actcttcctg
     7861 caactttcat ggtcatctca tagcgaccgt tttcgccacg aatacgtaag cccatatcgt
     7921 gcccacgcag ccagttatcc ggcgtttcgt agtaaatatt cagcaactgc acggggtcat
     7981 ggtgctcgcc gcccagcgta ttgagatggt cacgcaacgc ctcaacggca ctgtgattaa
     8041 caataaactt taattcgatt tcctgagcca tggcctttac ttatcggtta tgtcacataa
     8101 gtgacgatga acggcgaact taatgcgatc ttttttgtca gtagatagta ttttgcgcca
     8161 aattgccatg caacgggcaa tttgacgggc gtaaaagttt gaagcagtgg caagacctga
     8221 cacagaggat tccgattgat gtcgaatgct ttgcgtagcg acactgatgc cactactatc
     8281 gttccacttt ccatgacaat aacgacagcc tgatgccaaa attacgcctg atcggattaa
     8341 ctttactcgc acttagcgcc actgccgtct cacacgctga agaaacgcgc tatgtttctg
     8401 atgaactgaa tacctgggtc cgtagcggtc cgggagatca ttatcgcctc gtgggcacgg
     8461 ttaacgccgg cgaggaagtg accttattac aaactgacgc caacaccaat tatgcccagg
     8521 tgaaagacag ctctggccgt accgcctgga tcccgttgaa acaacttagc actgagccaa
     8581 gcctgcgctc ccgtgtgcca gatctggaaa atcaggtcaa aaccctgacc gataaactca
     8641 ccaatatcga taacacctgg aatcagcgca ccgcagaaat gcagcaaaaa gtggcgcaga
     8701 gcgacagcgt gatcaacgga ttaaaagaag aaaatcagaa attgaaaaac gagctgattg
     8761 tcgcgcagaa aaaggtcgat gccgccagcg tacagctgga tgacaaacag cgcaccatca
     8821 tcatgcaatg gtttatgtat ggcggtggcg tgctggggct tggcttgctg ctcggtctgg
     8881 tactgccaca cctgatccca acgcgcaaac gcaaagagcg ctggatgaac taaatcgcct
     8941 tcttcgttgc actgtcttat tattaggaat tgtgtatttg acgaagggat gatggcgtga
     9001 agatttatct ggtcggtggt gctgttcggg atgcattgtt agggctaccg gtcaaagaca
     9061 gagattgggt ggtggtcggc agtacgccac aggagatgct cgacgcgggc taccagcagg
     9121 taggccgcga ttttcctgtg tttctgcatc cgcaaacgca tgaagagtat gcgctggcac
     9181 gtaccgaacg gaaatccggt tccggttaca ccggttttac ttgctatgcc gcaccggatg
     9241 tcacgctgga agatgatctt aagcgtcgcg atctgaccat taatgcgctg gcccaggacg
     9301 ataacggcga gattatcgac ccgtacaacg gtctgggcga tctgcaaaat cgtctgttgc
     9361 gccatgtttc ccccgctttt ggcgaagatc cgttacgcgt tctgcgcgtg gcgcgttttg
     9421 ctgcgcgtta tgcccacctc ggttttcgta ttgccgatga aactctgacg ctgatgcgcg
     9481 agatgaccca tgcgggtgaa ctggaacacc tgacgcctga acgggtatgg aaagagacgg
     9541 aaagcgccct caccacccgc aatccccagg tgttcttcca ggtactgcgc gattgcggtg
     9601 cacttcgcgt tttattcccg gaaattgacg ccctgtttgg cgttccggcc cctgccaggt
     9661 ggcatccgga aatcgacacc ggtattcata ccttaatgac gctctcaatg gcggcgatgc
     9721 tgagtccgca ggttgatgtc cgtttcgcaa ctttatgcca cgatctcggt aaaggactga
     9781 cgccgccaga actttggccg cgtcatcatg ggcatggccc ggcgggtgtt aagttagtgg
     9841 aacaactatg ccagcgtctg cgcgtgccaa atgaaattcg cgatttagcc agactggtgg
     9901 ctgagtttca cgatctcatc cacaccttcc caatgctgaa cccaaaaacc atcgtcaaat
     9961 tatttgattc catcgatgcc tggcgtaaac cgcagcgtgt cgagcaactg gcgctgacca
    10021 gcgaggctga cgtacgcggc agaaccggtt ttgaatcagc ggactacccg caaggccgtt
    10081 ggttgcgcga agcctgggaa gtggcgcagt cagtgccgac aaaagcggtc gttgaagcgg
    10141 gatttaaagg tgtggagatt cgcgaggagc tgacccgacg gcggattgcg gcggtagcca
    10201 gttggaagga acaacgttgc ccaaagcctg actgagtata aaactaaacg ttaacacgaa
    10261 ccatcccctc gccccttcgg ggagagggtg aggggaacag accagcaccg gtgcgaacaa
    10321 tcacccccac cccggccctc tccctacaga gcgagggaga aaaccaggcc tgctcccgaa
    10381 ccttttctca atcaaaagaa cacgacgtac accgcagccg ccacaataaa gcgataaatg
    10441 gcgaacggga taaacgaaat gcgcttaatc aattgcagga aggttttaat cgctatcagc
    10501 gccaccacaa aagcggtgat aaacccaacg gcaaacatcg ggatatcgcc ggttgtcagg
    10561 aagccccagc ttttgtagag atcgagcgcc gttgcgccca tcatcatcgg taccgccagc
    10621 aggaacgaga actcggaagc agcgtaacgg ctcacgccca tcagcatccc acctgaaatg
    10681 gtggccccgg aacgggagaa acccggccac agcgccagac actggaaaca gccaatcata
    10741 aacgcctgac gataggtcat atcatcaaga cccggcgcac gcggctcttt cggcttcagg
    10801 cattcggcgg caatcagcaa caaaccgcca acgaccagtg catacatcac atttatcggg
    10861 ttaaacaatg acttaatcgt gtcgtggaac aacagcccca gtaccaccgc cggaatcatc
    10921 cccagcaaaa tgtggatcag cgttaaacga cctttgcttt ctccttcgtg ctgcaacggg
    10981 cggccaaagt ggatgccaat caggccaaac agacgccgcc agaacatcac tactaccgcc
    11041 agaattgatc ctaactggat cacaacttca aaggttttcg ccgtgtcgcc ctcgaacccc
    11101 aacaagtgac cgacaataat catatggccc gtgctggata ccggcagaaa ttctgtcaat
    11161 ccttcgacca cacccaatat tgccgctatc agcagcgagt gcatatcgct catcaataaa
    11221 cccctaaatt attaaaatgt accgcttgtc cggactactg cagtaagacc aggttataac
    11281 cgtttggttt aacagctgta aaattaatta ttttctttca gattattgcc acgctcaatg
    11341 attacaccaa cattcgccgc ccgcgccact gcgcctggct tgctgagttt gatgcgcacc
    11401 cacggcgagt tgaagcgtgc taacagcagc tccgccacct cttcagccac gcgttccacc
    11461 agcgcaaaac gcgccccctc gacgtggctg accaccgttt ctgcaatgtc agcgtaactg
    11521 aggcaatccg ccacatcatc acttttcgcc gctttacggt tatcccatgc catttcgata
    11581 tcgaacacta acttctgttc gatggtctgt tcccagtcgt aaacaccaat agtggtgatt
    11641 accgaaagtt gctctataaa tacaatatcc atcacgtcct gcctgctttt tggctaaccg
    11701 gataccactt ccggcgaaat gtgcgtatta tccacagatt catcgttgaa cacgaatttt
    11761 caaaacggaa cagcttatga gtgcaatcgc gcctggaatg atcctcatcg cgtacctctg
    11821 cggctccatt tccagtgcca ttctggtttg ccgcttgtgt gggctgcccg atccgcgaac
    11881 cagcggctcc ggcaatccag gcgcaaccaa tgtgttacgt atcggtggca agggagcagc
    11941 cgtagcagta ctgattttcg acgttctgaa aggaatgttg cccgtctggg gcgcgtatga
    12001 attaggtgtc agccccttct ggctaggctt aattgccatc gccgcctgtc ttggacacat
    12061 ctggcccgtt ttcttcggat ttaaaggagg aaaaggcgtt gctaccgctt ttggtgccat
    12121 cgcacccatt ggctgggatc tcaccggagt aatggcggga acctggttac tgaccgtgct
    12181 attgagcgga tactcgtcgc tgggagcgat tgtcagtgca ctgattgctc cgttttatgt
    12241 ctggtggttt aagccacaat tcaccttccc agtttcgatg ctctcttgcc tgatcctgct
    12301 gcgtcatcat gacaacatcc agcgtctgtg gcgtcgtcag gagacaaaaa tctggacgaa
    12361 attcaaaaga aagcgcgaaa aggatcccga gtgatttctg gtggatctac atgacctgat
    12421 agccttcatc gggcttgccc agccgttgct ggcaccatgc cgccagaaat tccacgcaga
    12481 cacgtaattt catgctgcga tagagcggct cccggtaaac agcccagata ttggcgcttt
    12541 gtgcatactc cggcaatact tgcaccagtt tgccactctc cagaaacggc aacacatccc
    12601 actcggaacg cagcataatc cctttgccct ccagcgccca ttgcaacact atctcgccgc
    12661 tattggagga aagatgcccg ctaaccttca ccgatttttt ctcctgaccg ttccccaact
    12721 cccatattcc gtgggtcata tcgcgctctt tggtcaccag acagtcatga cgacttaatt
    12781 cctgtaatga ttgtggctgc ggatattttt gcagatactc aggtgctgcg cataatattc
    12841 ttttattttt cgttaacaga tgcgcaatat aataatcagg aatttcgtca ttaatacgaa
    12901 tatccagatc aatattatcc tgcactaaat caatttgccg atcgaacaat tcaaaatgca
    12961 cctgtaactc aggataattg cgcataagtt cggtaatggc tggcgcaata tggctgcgcc
    13021 caaaaccaaa actgcaacca atacgaatca tcccctccgg gcgcgttttg atttgcgtga
    13081 cgtcatcgac cagt
//
LOCUS       DAZPEB010000116        13080 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZPEB010000116 DAZPEB010000000
VERSION     DAZPEB010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13080
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.117"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(14..520)
                     /gene="mug"
                     /locus_tag="ACHURJ_002784"
     CDS             complement(14..520)
                     /gene="mug"
                     /locus_tag="ACHURJ_002784"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFR1281666.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            645..720
                     /locus_tag="ACHURJ_002785"
     tRNA            645..720
                     /locus_tag="ACHURJ_002785"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:678..680,aa:Ile,seq:cat)
     gene            complement(774..1538)
                     /gene="nfeF"
                     /locus_tag="ACHURJ_002786"
                     /gene_synonym="yqjH"
     CDS             complement(774..1538)
                     /gene="nfeF"
                     /locus_tag="ACHURJ_002786"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFR1281667.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            1826..2449
                     /gene="nfeR"
                     /locus_tag="ACHURJ_002787"
                     /gene_synonym="yqjI"
     CDS             1826..2449
                     /gene="nfeR"
                     /locus_tag="ACHURJ_002787"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFR1281668.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(2603..4123)
                     /gene="aer"
                     /locus_tag="ACHURJ_002788"
     CDS             complement(2603..4123)
                     /gene="aer"
                     /locus_tag="ACHURJ_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFR1281669.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSIIASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            4540..5919
                     /gene="ygjG"
                     /locus_tag="ACHURJ_002789"
     CDS             4540..5919
                     /gene="ygjG"
                     /locus_tag="ACHURJ_002789"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFR1281670.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGSLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLLAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPQTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(5961..6293)
                     /locus_tag="ACHURJ_002790"
     CDS             complement(5961..6293)
                     /locus_tag="ACHURJ_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFR1281671.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            6512..7495
                     /gene="ebgR"
                     /locus_tag="ACHURJ_002791"
     CDS             6512..7495
                     /gene="ebgR"
                     /locus_tag="ACHURJ_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFR1281672.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLGIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAQEDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            7679..10771
                     /gene="ebgA"
                     /locus_tag="ACHURJ_002792"
     CDS             7679..10771
                     /gene="ebgA"
                     /locus_tag="ACHURJ_002792"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFR1281673.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHARDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            10768..11217
                     /locus_tag="ACHURJ_002793"
     CDS             10768..11217
                     /locus_tag="ACHURJ_002793"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFR1281674.1"
                     /translation="MRIIDNLEQFRQIYASSKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            11280..12713
                     /locus_tag="ACHURJ_002794"
     CDS             11280..12713
                     /locus_tag="ACHURJ_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR1281675.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            12847..12993
                     /locus_tag="ACHURJ_002795"
                     /pseudo
     CDS             12847..12993
                     /locus_tag="ACHURJ_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311988.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3149 a         3506 c         3269 g         3156 t
ORIGIN      
        1 tcggtggcgt tttttatcgc ccacgcacta ccagcgcctg gtccagctcg cgatacgctt
       61 caaccagttt ctctagtgaa acgcgactta aaccgctggg atttggcagc acccaaatct
      121 gcgtcgaacc aatggtgagc gtttgtttcc cccactgtgc accgcgctgg ctgaatccct
      181 gttcatatgc ttgtttgccc agaatcgcca acgcctgcgg ctgataatct tcaatttttt
      241 caatcagctt acgcccacct gcgtgcagct cctgctttga aacttcattg gcttgcaccg
      301 ttggacggtc taccagtttg gtgacgccac aacgataatc cagcagatgc tgtgcctcct
      361 gcggcttcaa ctgacggtcg gtaaacccgg cctgatatat caccttccag aagcgatttg
      421 ctggatgagc aaagggaaaa ccagtcccgg cggatgaaag cccagggttg ataccgcaaa
      481 acacgacccg taaccctgga gccaaaatat cctcaaccat atttactcct ttctaacatc
      541 accaggaaaa gtataacgga ttgaaaatac attgtttata aaaacagcag gcgcacggta
      601 atggctggat tgcgacacgg agttacttta taatccgcta ccatggcccc ttagctcagt
      661 ggttagagca ggcgactcat aatcgcttgg tcgctggttc aagtccagca ggggccacca
      721 gatatagcaa aggctgacga gaaatcgtca gcctttttct ttttatatct cagttacttt
      781 gcgtgccagt aagccgctgc acgtacccgc tgtgggtcat actgttccgc ttcaaagcgg
      841 cggcttaaat tcttaacgac tttaccttcg ccggttatcc agatgaagta atcatcggca
      901 gggatttgca tctgcgccag acgcgcatct accgcctgct catcatgtgc cagccattcg
      961 atattaaaac catcaaggtg cgcgagataa tcctgacagg cgttatcccg cacgctaacc
     1021 agcgcactaa cttgcggttt aacggcaagt ttgctcaacg tttccaggcg gcggcgcagt
     1081 gcgggcattc cggattcatc gcagacatac agctgatacg cgtaatcttc cggcaccacc
     1141 agcgaaccgc gcggaccagc caccgtaagt ttatcgcccg gttgcgcctg catcgcccag
     1201 ccgctggcga ccccaccgtc gtgaataaag aaatcgatcg ccagttcatg gcgtagttcg
     1261 tcatacagcg gcgtatagtc acgcgacggt gggcgtggtc cttccggcca gacgatgccc
     1321 tcttccgtta ccgttggcgg cacaaagtga gcgtcaggtt gaggaaagaa gagtttgctg
     1381 tgatcgtcaa agccgcgcga tgtaaaaccg tccagcgcct cgccgccgag gacaatgcgc
     1441 tgaaaaccgg cgctgatgcg ctcaacgcgt aacacagtca gttcacggaa gcgcagatca
     1501 ttgcgaacgc gctgcgggta gcggggggag ttattcattg ttatcgcctt cgtgatggta
     1561 atcagatata tctaaataat tttcgtaaat gataatgatt gttaatcatg ataaatgcaa
     1621 gcgattagta gaactgatat gtctatagtc tgataagacg aaccgcctct tctcaggcat
     1681 cattactcaa cgccggatgc ggcgtgaacg ccttatccgg cctacgtgtg agatgagtcc
     1741 acatttaaaa atcattttta ttcttgcaag agtgctcata tcagatataa attagatata
     1801 tctaatcaag aaaaaggagg ctgatatgag ccatcatcac gaagggtgtt gtaaacatga
     1861 aggccagcca cgccatgagg gctgctgcaa aggtgagaag tcagaacacg agcactgcgg
     1921 acacggtcac cagcatgaac acggtcaatg ctgcggtggt cgccacggtc gcggcggcgg
     1981 tcgtcggcaa cgtttctttg gtcacggtga attgcgtctg gtgattctgg atattctctc
     2041 gcgcgatgac agccacggtt acgaattgat taaagcgatt gagaatctaa cccaggggaa
     2101 ttacacccca agcccgggcg tcatctaccc aacgctggat tttctgcagg agcagtcgct
     2161 gattaccatc cgcgaagagg aaggtggtaa gaagcagatt gcgctgaccg aacaaggcgc
     2221 gcagtggctg gaagaaaacc gcgaacaggt ggagatgatt gaagaacgca tcaaagcgcg
     2281 ttgcgttggc gcggcgctgc gccagaaccc gcaaatgaag cgggcgctgg ataattttaa
     2341 agcggtgctg gatttacgcg tcaaccagag cgatatcagt gatgcacaaa taaaaaagat
     2401 cattgcggtg atcgaccgcg ccgcttttga tattacgcaa ctggattaat cgccgcatcc
     2461 gccagtggcg cggtgcaatt gccggatgcg acgcttgacg cgccttatcc ggcctacacc
     2521 cactacacac cccgcaggcc tgataagatg cgccagcatc gcatcaggca ttgtgctcca
     2581 accgccggat ccggcatacc gattaatgca gcaccgtcac cgcgtcttcc agtcggctgg
     2641 cgcggtgttt caccatcgcc gacacctgcg cactctcttc caccagctca gcatttttct
     2701 gggtgatcag gttaagctca tccactgcac gggtcaggct ggaaagccca tcggcctgtt
     2761 ccagcgttga atggctaatc tgggcgatca actgggtgac gtttttcacc tgtgccacaa
     2821 tatcttccat cgtccgtccg gcggcgtgta cctgctgcga accggattgc accttatcag
     2881 cactggcatc aatcagcttg cgaatatcgt tggcagcatt agcgctgcgg ctggctaaat
     2941 gacgcacttc ccctgccacc actgcaaaac ctttgccctg ttcgccggca cgcgccgctt
     3001 ccaccgccgc attcagggcc agaatattgg tctgaaacgc aatatcgtta atcagcgaag
     3061 taatggtgcc aatgcgctgg gtactgtcgg cgatatcgtc cattgtcttg atcaccgtgg
     3121 tcatcgcctc cccaccctgc actgccgcat tactggcgat gatagaaagt ttatcggcag
     3181 ccgacgccgt ggcagagttc tgtttcaccg acgccgccat ttggttcatg gtcgccaccg
     3241 tttgctgaac gttatcaact gtctgctggg tatgttcgtt cagttcatcg gtgcctttcg
     3301 ccagcgtctc actgccattt ctgacactgg acacctggct tgagacatcg ttaattagcc
     3361 aacggcacat caggccaagc tgccctaccg cgcgtaatgt cagccccagc tcatcgctgc
     3421 gattcaggtg ctcaacacta ttacgctctc cggtcgccac cttcagtgcc tgacgggcga
     3481 cattttctat cgggcgcaca atctgccatt caaaacaagc gcttgccaac aataccacta
     3541 acacacagag gaaatacgtc accaccgggg cagcaacaaa ccaaagcatg gccgccagca
     3601 agataaacat cagggtcatc actccacgcg cccgccagcg aagcggtaat gaaggcagtt
     3661 tacccagcca gcctttacgc accaccaggc ctttatgaat acgcttactg gtacgtccgg
     3721 cgttcagcgc tttgtacagc ggctccaccg ccgcaatctc ttcatccgtc gcccgggtac
     3781 gaatcgacat atagccactg atttttccct cgcgcaccat cggtaccgca ttggcccgca
     3841 cccaataatg gtcgccattt ttgcggcgat ttttcacgat gccgctccag ggctcccctt
     3901 ttttcagggt gaaccacata tccgcaaacg ccgcttttgg catatccggg tgacgcacca
     3961 tgttgtgcgg ctgcccttgt aactcttgca aggtaaagcc gctcacctgc acaaaagtgt
     4021 cattagcatg agtgatatag ctttgcagat cggtagtgga catcagagtg gtatcgtccg
     4081 ccagcggggt attttgctgg gtgacatacg gatgagaaga catgttcgcg tcctatgcag
     4141 gttatatggt tgttaacttc ttgtcagagt ttatgtcggc cccgctgcgg ttatctttaa
     4201 ccgattaatt tgatttagat cgcaatttgc gattaaagca caaatctaat tccttgattt
     4261 aaaatacttt cactctgtta ctatacgaaa acgttaatta tcttgcccaa aatcaggcaa
     4321 ttatcgccct gaaaacgtgc atttgcacag caatcatcaa atccataccc gacaaaaacc
     4381 gtgcaaaata acaacgaatg ttaacaaata gcattaaata ctgcgcaaat gataaccaca
     4441 tttgtgttta tcccgatttt cgcgatcgca gccggagtgg cgcaatccct gcaatactta
     4501 aatcggtatc atgtgatacg cgagcctccg gagcatattt tgaacaggtt accttcgagc
     4561 gcatcggctt tagcgtgcag cgcccacgcc ctgaatctca ttgagaagcg aacgctggat
     4621 catgaggaga tgaaagcact taaccgagag gtgattgaat acttcaaaga gcatgtcaat
     4681 ccggggtttt tagagtatcg caaatctgtt accgccggcg gggattacgg agccgtagag
     4741 tggcaagcgg gaagtttaaa tacgcttgtc gacacccagg gacaggagtt tatcgactgc
     4801 ctgggaggtt ttggaatttt caacgtgggg caccgtaatc cagttgtggt ttccgccgta
     4861 cagaatcaac ttgcgaaaca accgctgcac agccaggagc tgctcgatcc gttacgggcg
     4921 atgttggcga aaacccttgc tgcgctaacg cccggtaaac tgaaatacag cttcttctgt
     4981 aatagcggca ccgagtccgt tgaagcagcg ctgaagctgg cgaaagctta ccagtcaccg
     5041 cgcggcaagt ttacttttat tgccaccagc ggcgcgttcc acggtaaatc acttggcgcg
     5101 ctgtcggcca cggcgaaatc gaccttccgc aaaccgttta tgccgttact gccgggcttc
     5161 cgtcatgtgc cgtttggcaa tatcgaagcc atgcgcacgg ctcttaacga gtgcaaaaaa
     5221 accggtgatg atgtggctgc ggtgatcctc gaaccgattc agggtgaagg tggcgtaatt
     5281 ctgccgccgc cgggctatct caccgccgta cgtaagctat gcgatgagtt cggcgcactg
     5341 atgatcctcg atgaagtaca aacgggcatg gggcgcacgg gcaagatgtt cgcctgcgag
     5401 catgagaacg tacagccgga tatcctctgc cttgccaaag cgctcggcgg cggcgtgatg
     5461 ccgattggcg cgaccatcgc cactgaagag gtgttttcag ttctgttcga caacccattc
     5521 ctgcatacca ccacctttgg cggcaacccg ctggcctgtg cggcggcgct ggcgaccatc
     5581 aatgtgttgc tggagcagaa cttactggct caggctgagc aaaaaggcga tatgttgctg
     5641 gacggtttcc gtcaactggc gcgggaatat cccgatctgg tacaggaagc gcgtggtaaa
     5701 gggatgttga tggcaattga gtttgttgat aacgaaatcg gctataactt tgccagcgag
     5761 atgttccgcc agcgcgtact ggtggccgga acgctcaata acgccaaaac gatccgcatt
     5821 gaaccgccac agacactgac cattgaacag tgtgaactgg tgatcaaagc ggcgcgtaag
     5881 gcgctggcgg ccatgcgagt aagtgtcgaa gaagcgtaat atcaaatcgg atggcgatac
     5941 gacgtcgcca tccgatcttt ttatacgacg cgtacgcccg caggcatcat ccgctccggc
     6001 gttaacaaca cgctttcact gccgtcatcg gtttccgcac acaacagcat gcattccgac
     6061 gtttcaccac gcattttcgc tttttgcaga ttgcataaga ccaccaccgt tttccccatc
     6121 agctcttctt cgctgtagta cggcaccagg ctggtcacgg tttgcagtgt tttttccccc
     6181 acatcaacct gtacgatgta cagcttgtcg gcgttttcat ggcgtttcac ttccacaatc
     6241 tttccgacgc gcatttccag acgtgcaaaa tcagcgtaag ccacggtttc cattgtttcc
     6301 ttccttcggg taaaatttta ctaaactata gcaaaagttt ttctcaatcc tgtaggctaa
     6361 aaatggagaa tgcaggcgtg atcacattcg tgagccgctg tgttaccgtt acagcgtcaa
     6421 agaaacgcgc tttatttact gaaaacaggt gacccgataa gtacttcctc tacaatgggg
     6481 gcgcacatca gggaaagtaa aaaaggtaaa catggcaaca ctaaaagaca tcgcaatcga
     6541 agctggcgta tccctggcga cagtatccag ggtcttaaat gacgatccga cattgaatgt
     6601 gaaagaagag acaaaacatc gcattctcga gatcgccgaa aagctggagt acaagaccag
     6661 tagtgcccgc aaactccaga caggtgcagt caaccaacac catattctgg ctatctacag
     6721 ctaccaacag gagctggaga tcaacgatcc ttactatctg gcgatccgcc acggcattga
     6781 aactcagtgc gaaaagctgg gcattgagct caccaactgt tatgaacaca gcggattacc
     6841 agacattaaa aacgtcaccg gtattttaat tgtcggcaaa cccacgcccg cccttcgcgc
     6901 cgccgcctgt gcgttgaccg acaatatctg ttttatcgac tttcacgaac ccggcagcgg
     6961 ttacgatgcg gtggatatcg atctggcacg catcagtaaa gaaatcatcg acttctatat
     7021 caatcagggc gttaatcgta ttggttttat tggcggtgaa gatgagccag gcaaagcgga
     7081 tattcgtgag gtcgcctttg cggaatatgg ccgactgaaa caagtggtac gcgaagagga
     7141 tatctggcgc ggaggctttt ccagttcgtc gggttatgaa ctggcaaaac agatgctggc
     7201 gcaggaagac tatccgaagg cactgtttgt tgcttccgat tccattgcta tcggcgtact
     7261 gcgggcaatt catgaacgag gcctgaacat cccacaggat atttcgctta tcagcgttaa
     7321 cgatatcccc accgcgcgat ttacctttcc gccgctctcc accgtgcgca tccattccga
     7381 gatgatggga agtcagggcg ttaacctggt gtatgaaaaa gcccgcgatg gtcgcgcgct
     7441 gccgctgtta gtcttcgttc ccagcaaatt aaaactgcgc ggcacgaccc gttaaatccc
     7501 cttacacact gtccggcaat cgtttttgcc ggacagtgct gccgtttatt ttcgtgatcc
     7561 agttaaagta aatgcattta tctgctactt tttagtaaaa attttactaa actccccagc
     7621 aattacacaa actaccatca ccatgaatgg ttccgatttc tctctaccgg gaggccctat
     7681 gaatcgctgg gaaaacattc agctcaccca cgaaaaccga cttgcgccgc gtgcgtactt
     7741 tttttcatat gattctgttg cgcaagcgcg tacctttgcc cgcgaaacca gcagcctgtt
     7801 tctgccctta agcggtcagt ggaatttcca cttttttgac catccgctgc aagtaccaga
     7861 agccttcacc tctgagttaa tggctgactg ggggcatatt accgtccccg ccatgtggca
     7921 aatggaaggt cacggcaaac tgcaatatac cgacgaaggt tttccgttcc ccatcgatgt
     7981 gccgtttgtc cccagcgata acccaaccgg tgcctatcaa cgtattttca ccctcagcga
     8041 cggctggcag ggtaaacaga cgctgattaa atttgacggc gtcgaaacct attttgaagt
     8101 ctatgttaac ggtcagtatg tgggtttcag caagggcagt cgcctgaccg cagagtttga
     8161 catcagcgcg atggttaaaa ccggcgacaa cctgttgtgt gtgcgcgtga tgcagtgggc
     8221 ggactctacc tacgtggaag accaggatat gtggtggtca gcggggatct tccgcgatgt
     8281 ttatctggtc ggaaaacacc taacgcatat taacgatttc actgtgcgta ccgactttga
     8341 cgaagcctat tgcgatgcca cgctttcctg cgaagtggtg ctggaaaatc tcgccgcctc
     8401 ccctgtcgtc acgacgctgg aatataccct gtttgatggc gaacgcgtgg tgcacagcag
     8461 cgccattgat catttggcaa ttgaaaaact gaccagcgcc agctttgctt ttactgtcga
     8521 acagccgcag caatggtcag cagaatcccc ttatctttac catctggtca tgacgctgaa
     8581 agacgccaac ggcaacgttc tggaagtggt gccacaacgc gttggcttcc gtgatatcaa
     8641 agtgcgcgac ggtctgttct ggatcaataa ccgttatgtg atgctgcacg gcgtcaaccg
     8701 tcacgacaac gatcatcgca aaggccgcgc cgttggaatg gatcgcgtcg agaaagatct
     8761 ccagttgatg aagcagcaca atatcaactc cgtgcgtacc gctcactacc cgaacgatcc
     8821 gcgtttttac gaactgtgtg atatctacgg cctgtttgtg atggcggaaa ccgacgtcga
     8881 atcgcacggc tttgctaatg tcggcgatat cagccgtatt accgacgatc cgcagtggga
     8941 aaaagtctac gtcgagcgca ttgttcgcca tatccacgcg cagaaaaacc atccgtcgat
     9001 catcatctgg tcgctgggca atgaatccgg ctatggctgt aacatccgcg cgatgtacca
     9061 tgcggcgaaa gcgctggatg acacgcgact ggtgcattac gaagaagatc gcgatgctga
     9121 agtggtcgat attatttcca ccatgtacac ccgcgtgccg ctgatgaatg agtttggtga
     9181 atacccgcat ccgaagccgc gcatcatctg tgaatatgct catgcgatgg ggaacggacc
     9241 gggcgggctg acggagtacc agaacgtctt ctataagcac gattgcattc agggtcatta
     9301 tgtctgggag tggtgcgacc acgggatcca ggcacaggac gaccacggca atgtctggta
     9361 taaattcggc ggcgactacg gcgactatcc caacaactat aacttctgtc ttgatggttt
     9421 gatctattcc gatcagacgc cgggaccggg cctgaaagag tacaaacagg ttatcgcgcc
     9481 ggtaaaaatc cacgcgcggg atctgactcg cggcgagttg aaagtcgaaa ataaactgtg
     9541 gtttaccacg cttgatgact acaccctgca cgcagaggtg cgcgccgaag gtgaaacgct
     9601 cgcgacgcag cagattaaac tgcgcgacgt tgcgccgaac agcgaagccc ccttgcagat
     9661 cacgctgccg cagctggacg cccgcgaagc gttcctcaac attacggtga ccaaagattc
     9721 ccgcacccgc tacagcgaag ccgggcattc tatcgccact tatcagttcc cgctgaagga
     9781 aaacaccgcg cagccagtgc ctttcgcacc aaataatgcg cgtccgctga cgctggaaga
     9841 cgatcgtttg agctgcaccg ttcgcggcta caacttcgcg atcaccttct caaaaatgag
     9901 tggcaaaccg acatcctggc aggtaaatgg cgagtcgctg ctgacccgcg agccaaagat
     9961 caacttcttc aagccgatga tcgacaacca caagcaggag tacgaagggt tgtggcagcc
    10021 gaatcatttg cagatcatgc aggaacatct gcgcgacttt gccgtagaac agagcgatgg
    10081 tgaagtgttg atcatcagcc gcacagttat tgcaccgccg gtgtttgact tcgggatgcg
    10141 ctgtacctac atctggcgca tcgctgccga tggccaggtt aacgtggcgc tttccggcga
    10201 gcgttacggc gactatccgc acatcattcc gtgcatcggt ttcaccatgg ggattaacgg
    10261 cgaatacgat caggtggcgt attacggtcg tggaccgggc gaaaactacg ccgacagcca
    10321 gcaggctaac atcatcgata tctggcgcag caccgtcgat gccatgttcg agaactatcc
    10381 cttcccgcag aacaacggca accgtcagca tgtccgctgg acggcactga ctaaccgcca
    10441 cggcaacggt ctgctggtgg ttccgcagcg cccaattaac ttcagcgcct ggcactatac
    10501 ccaggaaaac atccacgctg cccagcactg taacgagctg cagcgcagtg atgacatcac
    10561 cctgaacctc gaccaccagc tacttggcct cggctccaac tcctggggca gcgaggtgct
    10621 ggactcctgg cgcgtctggt tccgtgactt cagctacggc tttacgttgc tgccggtttc
    10681 tggcggagaa gctaccgcgc aaagtctggc gtcgtatgag ttcggcgcag ggttcttttc
    10741 cacgaatttg cacagcgaga ataagcaatg aggatcatcg ataacttaga acagttccgc
    10801 cagatttacg cctctagcaa gaagtggcaa cgctgcgttg aagcgattga aaatatcgac
    10861 aacattcagc ctggcgtcgc ccactccatc ggtgactcat tgacttaccg cgtggagaca
    10921 gactccgcga ccgatgcgct atttaccggg catcgacgct attttgaagt gcattactac
    10981 ctgcaagggc agcaaaaaat tgaatatgcg ccgaaagaga cattacaggt agtggaatat
    11041 tatcgtgatg aaactgaccg tgaatattta aaaggctgcg gagaaaccgt tgaggtccac
    11101 gaagggcaaa tcgttatttg cgatatccat gaagcgtatc ggtttatctg caataacgcg
    11161 gtcaaaaaag tggttctcaa agtcaccatc gaagatggtt atttccataa caaataacaa
    11221 ctacggcggc aaaaggagtt tgccgccacc gctaccctac acattttcgg agatgtgtta
    11281 tgtctgatac caaacgtaat acaatcggca aattcggctt gctctcgctg acttttgctg
    11341 ccgtttacag ctttaacaac gttatcaata ataatattga gcttggactg gcctcggcac
    11401 cgatgttttt cctcgcgacg attttttatt ttattccctt ctgtctgatc atcgcagaat
    11461 ttgtttcgtt aaataaaaac tcagaagccg gtgtctacgc gtgggtaaaa agttcgctgg
    11521 gcggacgttg ggcatttatt actgcctata cctactggtt cgtaaacctg ttctttttca
    11581 cctcactgtt gccgcgcgtt attgcttatg cttcgtatgc cttcctcgga tacgaatata
    11641 ttatgacgcc ggttgccacc accattatca gtatggtgct gttcgccttc tccacctggg
    11701 tttccaccaa cggggcgaaa atgttggggc caattacctc cgtcacttca acgctgatgc
    11761 tgctgttaac gctctcctac attttactgg caggtacggc gctggttggc ggcgtacagc
    11821 ctgctgaccc catcaccgtt gacgcgatga tcccgaactt caactgggcg ttcctcggcg
    11881 ttaccacctg gatctttatg gccgcaggtg gcgcggagtc cgtcgctgtg tacgttaacg
    11941 acgtcaaagg cggttcgaaa tcgttcgtta aagtgatcat cctcgccggg atttttatcg
    12001 gcgtactgta ttccgtctcc tcggtgctga ttaacgtctt cgtcagcagc aaagagttga
    12061 aatttaccgg cggatcggtg caggtattcc atggcatggc ggcgtatttt ggtctaccgg
    12121 aagcgttgat gaatcgcttt gtcggtctgg tgtcctttac cgcgatgttc ggttccctgc
    12181 tgatgtggac cgcaacgccg gtgaaaattt tcttctccga aatcccggaa ggcatctttg
    12241 gtaagaaaac cgtcgaactg aacgaaaacg gcgttccggc gcgcgcagcg tggatccagt
    12301 tcctgatcgt catcccgctg atgattatcc cgatgctcgg ttccaatacc gtgcaggatc
    12361 tgatgaatac tattattaat atgaccgccg cagcgtccat gcttccgccg ttattcatca
    12421 tgctggctta cctgaattta cgcgccaaat tagatcacct gccacgcgat ttccgtatgg
    12481 gctcccgccg caccggtatt atcgttgttt caatgctgat tgcgatattt gccgtagggt
    12541 ttgtcgcttc gacattcccg actggcgcga atattctgac catcattttt tataacgtcg
    12601 gcggtattgt tatcttcctt ggctttgcgt ggtggaaata cagtaaatat ataaagggat
    12661 taacggctga agagcgccat attgaagcga cgccagccag caatgttgat taagcagaat
    12721 aataaaaaat gagcaggcat tttccctctt cctggatgag gagagggaaa atagtttctg
    12781 cctttatatt tttataatta caacgataaa aggctgtact ttttctttag ctcatggatt
    12841 aacacaatga aattaatcac tgcaccatgc agagcattac ttgctctgcc gttttgctac
    12901 gccttttctg cggcaggaga agaagcacgt ccggcagaac atgacgacac aaaaacaccc
    12961 gcaattacct cgacatcttc tccttcattt cgttggtaat gctgccaact tactgattta
    13021 gtgtatgatg gtgattttaa ggtgcttgcg tggcttccat ttccatcaga tgtccttcct
//
LOCUS       DAZPEB010000117        12964 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZPEB010000117 DAZPEB010000000
VERSION     DAZPEB010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12964
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.118"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..135
                     /locus_tag="ACHURJ_002796"
     CDS             <1..135
                     /locus_tag="ACHURJ_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1281676.1"
                     /translation="IMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT
                     "
     gene            505..1329
                     /gene="gadX"
                     /locus_tag="ACHURJ_002797"
     CDS             505..1329
                     /gene="gadX"
                     /locus_tag="ACHURJ_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFR1281677.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
     gene            1697..2425
                     /gene="gadW"
                     /locus_tag="ACHURJ_002798"
     CDS             1697..2425
                     /gene="gadW"
                     /locus_tag="ACHURJ_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFR1281678.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSLGTFS"
     gene            complement(2788..5901)
                     /gene="mdtF"
                     /locus_tag="ACHURJ_002799"
     CDS             complement(2788..5901)
                     /gene="mdtF"
                     /locus_tag="ACHURJ_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFR1281679.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIGAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5926..7035)
                     /gene="mdtE"
                     /locus_tag="ACHURJ_002800"
     CDS             complement(5926..7035)
                     /gene="mdtE"
                     /locus_tag="ACHURJ_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFR1281680.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            7143..7421
                     /locus_tag="ACHURJ_002801"
     CDS             7143..7421
                     /locus_tag="ACHURJ_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1281681.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(7422..7949)
                     /gene="gadE"
                     /locus_tag="ACHURJ_002802"
     CDS             complement(7422..7949)
                     /gene="gadE"
                     /locus_tag="ACHURJ_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFR1281682.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITLTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(8748..9320)
                     /gene="hdeD"
                     /locus_tag="ACHURJ_002803"
     CDS             complement(8748..9320)
                     /gene="hdeD"
                     /locus_tag="ACHURJ_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFR1281683.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            9575..9907
                     /gene="hdeA"
                     /locus_tag="ACHURJ_002804"
     CDS             9575..9907
                     /gene="hdeA"
                     /locus_tag="ACHURJ_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFR1281684.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            10023..10349
                     /gene="hdeB"
                     /locus_tag="ACHURJ_002805"
     CDS             10023..10349
                     /gene="hdeB"
                     /locus_tag="ACHURJ_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFR1281685.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            10413..11060
                     /locus_tag="ACHURJ_002806"
                     /pseudo
     CDS             10413..11060
                     /locus_tag="ACHURJ_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(11102..11632)
                     /locus_tag="ACHURJ_002807"
     CDS             complement(11102..11632)
                     /locus_tag="ACHURJ_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFR1281686.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDGMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEAAVSLSRKQHQVLSCIANQMTTEDILEKLKITLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(11788..12354)
                     /gene="slp"
                     /locus_tag="ACHURJ_002808"
     CDS             complement(11788..12354)
                     /gene="slp"
                     /locus_tag="ACHURJ_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFR1281687.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            complement(12709..12964)
                     /locus_tag="ACHURJ_002809"
                     /pseudo
     CDS             complement(12709..12964)
                     /locus_tag="ACHURJ_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3583 a         2845 c         2912 g         3624 t
ORIGIN      
        1 attatgtgtc gtcgcggctt cgaaatggac tttgctgaac tgttgctgga agactacaaa
       61 gcctccctga aatatctcag cgatcacccg aaactgcagg gtattgccca gcagaacagc
      121 tttaaacaca cctgataaca taacgttgta aaaaccgaat gcccagcctt taaaaaaaca
      181 gctgggcatt cggttgctta ttaatcgcaa taatatattg gctgtctatt catcgtgttg
      241 ataagatata tgttatatga atgtttatta aatgtagcga ttgcctttaa tgtcacgaaa
      301 agggattatt tgcttgctat taatttcact gcgttcttaa acgcgttttt gtattataaa
      361 tccagtcatc ctgcccgtag aacatcacac atcatcatcc tgttctcccg cttcgtttaa
      421 atttatttat caatcaattt gacttaagag ggcggcgtgc tacattaatt aacagtaata
      481 tgtttatgta atattaagtc aactatgcaa tcattacatg ggaattgtct aattgcgtac
      541 gcaagacata aatatattct caccatggtt aatggtgaat atcgctattt taatggtggt
      601 gacctggttt ttgcggatgc aagccaaatt cgagtagata agtgtgttga aaattttgta
      661 ttagtatcaa gggatacgct ttcattattt ctgccgatgc tcaaggagga ggcattaaat
      721 cttcatgcac ataaaaaaat ttcttcatta ctcgttcatc actgtagcag agatattcct
      781 gtttttcagg aagttgcgca actatcgcag aataagaatc ttcgctatgc agaaatgcta
      841 cgtaaaagag cattaatctt tgcgttgtta tctgtttttc ttgaggatga gcactttata
      901 ccgctgcttt tgaacgtttt gcaaccgaac atgcgaacaa gagtttgtac ggttatcaat
      961 aataatatcg cccatgagtg gacactagcc cgaatcgcca gcgagctgtt gatgagtcca
     1021 agcctgttaa agaaaaaatt gcgcgaagaa gagacatcat attcacagtt gcttactgag
     1081 tgtagaatgc aacgtgcttt gcaacttatt gttatacatg gcttttcaat taagcgagtc
     1141 gcagtgtcct gtggatatca cagcgtgtcg tatttcattt acgtctttcg aaattattat
     1201 gggatgacgc ccacagagta tcaggagcga tcggcgcagg gattgccgaa ccgtgactcg
     1261 gcggcaagta ttgttgcgca aggaaatttt tacggcactg accgttctgc ggaaggaata
     1321 agattataga attttactca gacataaaaa aaacctggca taggggaccg ggaagaggat
     1381 agtctgccgt ctccagacta ataaaccgct ataacactcc ctgttggcac gggaaacttt
     1441 gtgctctcag taagttaaat ataactttta ctggaaataa gatcagccat ttttttataa
     1501 acataagcta tacgctgtgc gaaaatatat tcgtgctgca tttacttatt atcaattaac
     1561 tgttatgcaa aactactttg tggataaatt ttggtcctac caaatctggc agtttttgcg
     1621 ctaagaaaca gtctggcaac atttcattag tatactgaaa ttgaaataat cgcagtatga
     1681 aatataaggg ataatcatga ctcatgtctg ctcggtgatc ctcattcgtc gttcattcga
     1741 tatttatcat gaacagcata aaatatcgct gcataacgag agtatcgtgc tgctggagaa
     1801 aaatttggca gacgattttg cgttttgttc accggatacg cgacgactgg atatcgatga
     1861 gctgacagtt tgccattact tacaaaatat tcgtcagcta ccacgcaatt tagggttaca
     1921 tagcaaagac cgtctattaa ttaaccagtc accccccatg ccgctggtga cggcgatttt
     1981 tgatagcttc aatgaatccg gggtaaattc accgatactg agcaatatgc tctacctttc
     2041 ctgtttatcg atgttttctc ataagaaaga actgatcccc ttacttttca atagcatcag
     2101 cactgtttca ggaaaagttg aacgccttat tagctttgat atcgccaaac gttggtatct
     2161 gcgcgatatc gcggaaagaa tgtataccag cgagagtctc atcaaaaaaa agttgcagga
     2221 tgaaaatacc tgtttcagta aaatattact cgcttccagg atgtcgatgg ccagacgatt
     2281 actcgagtta cgtcaaattc ctctgcatac tattgcagaa aaatgtggct atagcagtac
     2341 gtcgtacttt ataaacacat ttcgacaata ttatggtgta acgccacatc agtttgcgca
     2401 acattcgcta ggtacctttt cctgacatat tttgcatttg aatattggtc aggatctcac
     2461 acctgcttca tgtgaaactc ttccctgatg atttctgccg ggctaccggc tagttctctt
     2521 tcgcagtttg ccaggcggta taggtatttt caggaggaaa agactcggca tgtttgggat
     2581 tattaagctg acaattcata cgattacggg aatgtgggtc agtattgtgc tgttcaaatt
     2641 gatgacgaat ggctggagtg gtttctactt tcagtgctgt gtactaagcc tggtattcct
     2701 gacggttagc tggttgttga gtggtgaatg gttagcagga aagagtaagg ctgaaccttc
     2761 acgttcaacc ttactctcat ttacacgtta cgctttttta aagcgggcaa agagatgttc
     2821 cactacaacg aaaaagaccg gaacgaagta aattgccagc actgttgcgg caaacatccc
     2881 gcccatcacg ccggtaccta ccgcgttttg cgcgccagaa ccggcaccat gactgataac
     2941 cagcggcagc acgccgagaa taaaggccag agaggtcatc aggattgggc gtaaacgcat
     3001 ccgcgccgct tcgatgattg cccctatcgg cgttttccct tctttctgca tcatctcaac
     3061 ggcaaattcg acaatcagga tggcgttctt ggcggaaagc ccgatggtgg tcagcaaacc
     3121 aacctggaag tagacgtcat tacttaagcc gcgcagatcg gtggccagta atgcgccaac
     3181 gacgcctaac ggaacaacca acatcaccga gaacggaatt gaccagctct catagagtgc
     3241 ggcgagggcg aggaacacca cgaccagtga aatggcatac agtgcaggag cctggtttga
     3301 ggataacgct tcctgatacg acagtccggt ccatgagtag ccgacgcctg ccggaagttt
     3361 agcgaccagg tctgccataa atttcatagc gtcaccggta cttttcccgg ccgccgcttc
     3421 acctaaaatc tccattgacg ggatgccgtt gtagcgttcc aatcgcggtg aaccataggt
     3481 ccattcggta gacgagtagg cagaaagcgg tgccatcgtg ccagaggcgt tgcgtacata
     3541 ccattggttg atgttatccg gcaacatacg gaacggcgtg cctgcctgga catacacttt
     3601 tttcacccgc ccctggttga ggaagtcgtt cacgtagctg ctgccgaagg cggtggaaat
     3661 tgtctggttg atatcagaca gcgccacacc cattgcctca gctttcgcgg cgttgacgtt
     3721 cactttgaac atcggcgtat cttccaggcc gttcgggcgt accccggtga cctggtcagg
     3781 cgattgcgct gccagtgata acagctcgtt tcgtgcctgg gttagttttt cgtgccccag
     3841 gttaccgttg tccagcagtt ccatatcaaa acctgacgcg gtacccagtt cagccaccgc
     3901 gggtaagttg aacgggaaga cgacggcttt attgatactg cttaacgcaa tcattgcccg
     3961 ctgaatgatc gcggtaaccg agttttcctc accgacacgt tcagaccacg gcttgagact
     4021 gataaacgcc aggccgttgt tttgcccctg gccgctgaag ccaaagccgc caacggtaaa
     4081 caccgactga acattatctt tctcttttgt aagataataa tccgtcacct gttgcagcac
     4141 tttcgtggtg ttaaccatgg tggcaccgga aggtaactgc gcggtggtca taaatacccc
     4201 ctgatcctct tctggtaaga aagaggtcgg tgtacgcagg aacagcaccg ccatcccggc
     4261 gcaaatcagc aggtagacca ccatgtagcg accggtacaa cgcaacagcg agcgggtgct
     4321 atcggtatag tgctgagttg atttttcaaa cagcgtgttg aagcgtgcga acagggcgtt
     4381 aggtttgtga ccgccttccg gcgcggcttt cagaatggtg gcgcacaggg caggggtcag
     4441 gctcattgcc acaaatactg aaagcagcat ggaggagatc agcgtgatgg agaactggcg
     4501 gtagatctcc ccggttgcgc cgctcataaa ggccatcggc ataaacactg cggaaagaac
     4561 aacagcaata ccgaccagcg cacgttggat ctgccccatc gatttatgcg tcgcttcctt
     4621 cggtggtagc ttatcttccg caatgacacg ctcgacgttc tccaccacca cgatggcgtc
     4681 atccaccagt aaccctatcg ccagcaccat cccgaacata gttaacgtgt tgatggtgaa
     4741 accgaccgcc gacaagatcg caaacgtccc gagaataacc accggtacgg caatcgtcgg
     4801 gatgattgtg gcacggaaat tctgcaaaaa cagatacatg accaggaaga cgaggatgat
     4861 agcctcaacc agtgttttaa aaacttcctg aatagaaatt tcgataaacg gcgtggtgtc
     4921 gtaaggataa accgtcttca gacttgcggg gaaataggct gataagcggt tcagctcctc
     4981 tttgactgcc cgcgaggtat ccagggcgtt tgctccggta gccagtttga tggcgatccc
     5041 ggcagcaggt ttgccgttat agcgcgccac ggtggaataa tcttccgctc caagttcgac
     5101 gcgggcgaca tcacgcagca gcacttgcga accatcttgc tgaactttca acaggatttt
     5161 gccaaattct tccggcgttt gcagacgcgt ctgcacaatg atcgaggcgt ttagttgctg
     5221 gtctgccgcc tgtggcatgc cacccagttg accaccggaa atctggttgt tttgcacctt
     5281 aatctgggaa ataacatcgg aaggtaccag attgtattta ttgagttttt gcgggtccag
     5341 ccagatacgc atggcgtact cggaaccaaa gagctgtacg ctaccaacgc ccgcggtacg
     5401 gcttagcggg tctttgatat tagacgctac atagtccgcg atatcgtact ggttgaggct
     5461 gccgttatca gaaataaacg ccgctaccat cagaatatta ctgctcgact tatcaacgct
     5521 aatcccctgc tgctgcaccg cttcaggtaa tgaaggcata gcgagttgca gtttattttg
     5581 cacctgaacc tgtgcgatat caggagatgt cccagtctcg aaggtcagag tgatagaggc
     5641 attacccgcc gcatcactgg ttgaagacat gtacatcagg ccatcaagcc cattcatatt
     5701 ttgctcaatc acctgagtga ccgagtcttc taccgtttgc gcatcggcac ctggataggt
     5761 agcgctgacg gtaatggtcg gtggcgcaat ctgcggatac tgcgcaaccg gtaagttcat
     5821 gatcgccaga ccacctgcaa gcatcataat aatggcaagt acccaggcaa aaaccgggcg
     5881 atcaataaaa tagttagcca tgaaagtccc cttaagcctg caacgttatt gtttcgattc
     5941 ggtgctggcg ttttcctggc tggaggaaat tgctcgtgct ttgatacccg gacgaatgcg
     6001 ttgcaaaccg gaaacgatca cccgatcgcc agcctgcaag ccagaggtga caacccactg
     6061 gtcgccgatg gctttgctgg cttcaatttc gcgtagctgc acaacatcgt ctttatccag
     6121 aatgagcgcc gttgctttac cctgggcgtt gtgggtgacg ccttcctgcg gcaccagtaa
     6181 tacattctgg cggctacctt catccactaa tgccgtgacg tacatgccag gcagcaagtc
     6241 accatttggg ttggggaaaa tcgcccgtaa cgtcacggag cccgtggttt catccaccgt
     6301 cgggtcggag aatttcagtg tgccggtctg gctgtagcgt ttaccatttt ccagattgag
     6361 ctgtactggc gtactgccct gaacctgttt gatttgccca ctggcgacct cttctttcat
     6421 gcgcaagaaa tcctgcaccg actgcgtgag atcgacataa atcgggtcca gacgttgtac
     6481 ggtaaccagc gaatctgcct gattagcggt aacgagtgcg ccgacggtca ccgacgattt
     6541 cccgctgacg cccgtaatcg gcgaggtgac attcgcgtat tgcagattga tcgtcgcctg
     6601 ttcaacagcc gctttggcga cggtgacatt ggcttctgct tcattcagct gggtgcgcgc
     6661 ggtgtcgtaa tcctgacggc taacgtagtt ggtcttcagc aacgatgcct ggcggttaaa
     6721 ggtgatgcgg gcattgctgg cggtagagag cgctttcgcc agcgagcctt tggcggagtt
     6781 tagctcggcc tgtaaaggtg caggatcaat ctgatacagc gaatcgccct ggttcacttt
     6841 atcgccttcg ataaagttgc gtttaatgat aataccgccc acctggggac gtatctcggc
     6901 aacttcataa ggaacggttc taccgggcaa ttcgctcaac acattgaccg aaccggggga
     6961 gagtgtgacg acaccgacct caggcgtcat ggcggcggcg ttttccgccg atttgtcatc
     7021 gcaggcggtg agcatcgcgc cgcagaataa caacggtatt aacagctttc ttcttctgtt
     7081 cattttagtc cctgaaaatt cttgagaaat aacggttctt taaattgcgc gcgaggagag
     7141 atatgaggga tttacagacc agcggcatag tggggttgtc agccagtaag gtgggataca
     7201 ggcacagtga tcgacatggt gaggtcaacg acaaaaaaaa tgttgccgtt ctgccaacag
     7261 ttcctgccag cattcgggca acgaagggtt ctactggtgg atacacatac caggggaata
     7321 aagtcgtttt agcctctcct ttagtaatca ccgggggtaa tgaaatcagc atctgcatcc
     7381 ctcgtcatgc cagccatcaa tttcagttgc ttatgtcctg actaaaaata agatgtgata
     7441 cccagggtga cgatgtcgct catacgtctt agtttcaact tgtacataat gttgaatttc
     7501 tgaatcttta atgtttgttg cgaaatattg agcgtcaacg tgatattgct ttcattttta
     7561 tttttcagaa tcagttgcat aattttgaac tgattatgtg aaaaacataa ttgtgaattc
     7621 ttatggggca agtgtttacc ataagtaatc tcaagaagat ttttgatact ttctttgcgg
     7681 ctaacaaaat aaataggcgc tttagctttt agtttactga tgtgataagg tgccaaaaca
     7741 ataattctct cggcatctaa tttctccaga aatttaatcg cttcttcatc aaggatatga
     7801 ttgtgatagg tatcaatgat aaccttgaag ggtttattgc ctactttttt gatctctgac
     7861 agggaattga tttttatcat ttcgtgatta tctttcaact gccaaaagcc ctgtaaaaga
     7921 aaagaatctt tcgtcatgag aaaaatcata acttgctcct tagccgttat cgtttcgaac
     7981 atgtcatcct tttcattatt tacatccttg tccgaatcgt tgttcaatat agtaaacgcc
     8041 tataagaact ttacccttcg tgaactaata cgtctattaa cactaatcaa tacttcagat
     8101 ttatccatca cgcactccat atattgatcc taagtaagat tcctggttgt tatcagcttg
     8161 taaatagtaa acaaaaagtt aacgtttgcg gattactgcc caagaataag ttatcaataa
     8221 ccagtttgtg atctctgaag aatattacta aagttaaaat gtaatccgat ttaaatatcg
     8281 agtctccttg tttcgactta agctggcaat tggattgcca gctttctttt aacgcattga
     8341 aattacctga gtaaataacg cattcatgtc ttcaggtttt tatatagcgt ctggagacac
     8401 ggatatttat gcaatgtaat caacgccagt tgcaaaaaaa tgaacccgga ttacttattt
     8461 aaccgcccat agcatcaacg cttacggaac tctttgttca ctttcccaca gcacttttaa
     8521 aacattaaac ctgactacgg aaaatatcag ccatgataat gtttgaaatg gctaatttgc
     8581 catggagtga aaaaaattag atgaaattca gtaggttgaa ataatcacta gcaggtaatt
     8641 atttcaatga tagtgcgcaa ttgatctaca acactgcgta gcggagagag tattaatcgg
     8701 atcatagtca catcaagtga ctatgatccg ggtgacaacc ggggtaatta ttgctgctta
     8761 acgaacaaac tggcgaagct gaacaggctg gcggcgctaa atatcagttc aattcccacc
     8821 agtgtggaaa ccagcgttac agacaccatc ggcgttgcac caaggaatat ccaggcaatg
     8881 acgatatcca gcacaccaat aacgagctgt agccagctgc ctttcattga acgctgacga
     8941 taccaactca tcaggcgaat aacccctgca acacagaaca aaccggcaat aaatgccgca
     9001 atggcaaaaa tgcccagctc cggtgcgcgg atgaagaaat agccgatcaa taaataggcg
     9061 actgcgacga ggaaaccgga taataccggc cagaaattat gactgcggtt gctgaataac
     9121 ccgacaataa gcgcaatacc cgagcagatt aataatgcac ccactactgt gcttaaaata
     9181 tcgccagaga cgaacgggaa actgatacac agcaacccga cgataaacag cagcacggca
     9241 ataaactgga ttgctctgcg atgtttttta agcatctcca gatcaaactt caaaattgtt
     9301 gccttatcta tatataacat agaaccaccc tataaaatta agaagaaaat cccctgctat
     9361 caatctatgc caaaaacgcg tctaagaatg cagtcgattt aataaaaatt tcctaattgc
     9421 agtatctgat gcatctgtaa ctcattgtat tgaaataaaa atatctgatt ttgatatttt
     9481 ccatcaacat gacatataca gaaaaccagg ttataacctc agtgtcgaaa ttgatttgtg
     9541 acggctcttt cactttatag ttgaggatat tacgatgaaa aaagtattag gcgttattct
     9601 tggtggtctg cttcttctgc cagttgtgag caatgcagcg gatgcgcaaa aagcagctga
     9661 taacaaaaaa ccggtcaact cctggacctg tgaagatttc ctggctgtgg acgaatcctt
     9721 ccagccaact gcagttggtt ttgctgaagc gctgaacaac aaagataaac cagaagatgc
     9781 ggttttagat gttcagggta ttgcaaccgt aaccccagct atcgttcagg cttgtactca
     9841 ggataaacaa gccaacttta aagataaagt taaaggcgaa tgggacaaaa ttaagaaaga
     9901 tatgtaattc cgggaatgcg ttacatcgta cttccttgca tattgaacag gccggaatat
     9961 cttctttaaa agcagctatt cctcctgttc atatataatc tatatattga atgggttaca
    10021 aaatgaatat ttcatctctc cgtaaagcgt ttatttttat gggcgctgta gcggctttgt
    10081 cactggtgaa cgcacaatct gcgctggcag ccaatgaatc cgctaaagat atgacctgcc
    10141 aggaatttat tgatctgaat ccaaaagcaa tgaccccggt tgcatggtgg atgctgcatg
    10201 aagaaacagt atataaaggt ggcgataccg ttactttaaa tgaaaccgat ctcactcaaa
    10261 ttcctaaagt gatcgaatac tgtaagaaaa acccgcagaa aaatttgtat accttcaaaa
    10321 atcaagcatc taatgacttg ccgaattaat gaggtacaag taaaaagtag tagcaagttg
    10381 agccatcttg ctgctccttt ttgcattttt atatgacagc agaatttatt atacgtctta
    10441 tactcgcggc aattgcctgt ggcgctattg gcatggaaag gcaaatgtga ggcaaaggag
    10501 cagggttacg cacacatgta ttaattggca tgggaagcgc cctgtttatg attgtttcga
    10561 aatatggttt tgctgacgtg ctgtctttag atcacgtcgg actcgacccc agccgtattg
    10621 ctgctcaggt ggtgacgggc gtcgggttta tcggtgcagg taacattctc gttcgtaacc
    10681 aaaatattgt aggtttgacg acggcagcgg atatctggat gaccgccgct ataggtatgg
    10741 ttattggcag tggtatgtac gaattgggta tgtatggttc agtgatgacc ttgctggtgc
    10801 tggaagtctt ccatcaatta accttccgcc tgatgaacaa aaattaccat ttacagctga
    10861 cattagtgaa cggtaatacg gtatcaatgc tcgactggtt taagcagcaa aaaataaaaa
    10921 cggatttagt ttcgttgcag gaaaatgaag atcatgaagt ggtcgccatt gatatccagc
    10981 ttcatgccac aacgtcgata gaagatctgt tgcgattatt aaaaggtatg gcaggggtga
    11041 aaggcgtttc aattagctaa tctgtgtggc agcgtagtca gactcaccgt aagcctgaaa
    11101 ttcacaccag ataatcaata tgctgatggc gtaccagctc attgatccgc ttaagattga
    11161 ggatcatcat gatattgtgt ttatggcagt agaacgtttt tagcgttatt ttcagtttct
    11221 ccagaatatc ctccgttgtc atttgattcg caatgcagct cagaacctga tgttgcttgc
    11281 gcgaaagtga cacagccgct tcaggcacag agcagtaact gccattgatg agtgcgacaa
    11341 aatcgatgat agtgatagtc cgcggaacaa aggtcaccgg cactttcccc aggcagcgtt
    11401 taatattaaa gggggagaaa ataatcacat ggacaggttt taatcgttca atttgagtga
    11461 gaaaattaga atgaaataca ttattcatta atgtatcaat aatgacaaag gcattctgat
    11521 gcaacattcc gtcgatctct tcttcgttct gtatatgaac gacattacct ttgctcaaaa
    11581 tgtttttcat cgcggtgaag aacatcgtat ccctggtaat tataagaaac ataaccgact
    11641 cctggtgcgg acattaattt tataatcagt ttgccaaagg taattaacag gacagggctt
    11701 ataataataa tagttagctt gctgtgtatt aattttatgt aatgcacgat gcagatgctg
    11761 agaaagcatc tgcatctttc ggtggtgtta tttgaccagc tcaggtgtta cctgactcac
    11821 cgcattggtg tagtaaggcg caccccagcc cggttccggc cagaatgcac catagccgta
    11881 atcccatagg ttataggtgg tattaaccac ttctctcaaa tgccacacct ggatgccctg
    11941 catattcact tccaggaagt tatacgggac tttattgata aagccaggtt gttcaccctg
    12001 aatggtaccg aggatggtaa caaagtgatt acgatagttc actgggtcaa ggaagccgct
    12061 ttgtctggcg agcagtcggc cctgatagtt ggcttcaata tcaggtttcg catagctatc
    12121 caacggtaat acagcgattt ctaacaacgt atccgttttg ccattgataa cgttgataac
    12181 cttcccacca aagcgcgctt gttgaccaac atataacccc ggctggttat gaacagcaac
    12241 aaaacttttt tgaatatcag gttggttatt gcctttgata ttttgcggaa ttgaactaca
    12301 tgcggcaagc aaaaatgaaa ggctgaggat gagtgcacct tttgtcatgt tcatgttact
    12361 atccttatca acaaatatta aaaccataat agatatttct ttatagtttt catctgattc
    12421 tgaggcgata tgcctgatag taacgtaaac ctgtctgatg tttatatccc ttttgtttag
    12481 ttcaattcca ttgattttgg ggcgtggcat aagtatccat cgccccataa tataatcctt
    12541 tgttttaaaa ttacataaaa tctattttta ttcaataatt ataaagtgct aataatcaat
    12601 attatggtat tatattctga taatgatttg ataagcgaat tcttttgcca gatggtattt
    12661 ctatttccac cccataattt atatcttttt cattaggaag tggcctcttt aaataaggcg
    12721 tgtgtgacat tttttttggg ttaagcgtct cgaattgaaa tacttcgaac tgatattcag
    12781 acatttctgc ccatgtttga attttattgt atcgagagag tgtcgtagag gctttaactt
    12841 gcttataaca ttgtttttgg aattcttgct gatattttaa tatgtcattg tatgaaagat
    12901 tggttatcct ggcctctaaa aatttaatta ttgtcgaagc aatttccgct tttgctcgta
    12961 aaaa
//
LOCUS       DAZPEB010000118        12775 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZPEB010000118 DAZPEB010000000
VERSION     DAZPEB010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12775
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.119"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..467
                     /locus_tag="ACHURJ_002810"
     CDS             <1..467
                     /locus_tag="ACHURJ_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712350.1"
                     /note="type IV secretion system coupling protein; similar
                     to F plasmid TraD; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HFR1281688.1"
                     /translation="ERPKVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPDV
                     PEVVSGEDVTQAEQPQQPVSPVINDKKSDSGVNVPAGGIEQELKMKPEEEMEQQLPPG
                     ISESGEVVDMAAYEAWQQENHPDTWQQMQRREEVNINVHRERGEDVEPGDDF"
     gene            complement(476..874)
                     /locus_tag="ACHURJ_002811"
     CDS             complement(476..874)
                     /locus_tag="ACHURJ_002811"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFR1281689.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(874..1101)
                     /gene="vapB"
                     /locus_tag="ACHURJ_002812"
     CDS             complement(874..1101)
                     /gene="vapB"
                     /locus_tag="ACHURJ_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HFR1281690.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSADFMDNREQPGMQERESF"
     gene            1183..6460
                     /gene="traI"
                     /locus_tag="ACHURJ_002813"
                     /pseudo
     CDS             1183..6460
                     /gene="traI"
                     /locus_tag="ACHURJ_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
     gene            6480..7226
                     /gene="traX"
                     /locus_tag="ACHURJ_002814"
     CDS             6480..7226
                     /gene="traX"
                     /locus_tag="ACHURJ_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HFR1281691.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            7281..7841
                     /locus_tag="ACHURJ_002815"
     CDS             7281..7841
                     /locus_tag="ACHURJ_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFR1281692.1"
                     /translation="MTEQKRPVLTLKRKTEGTATVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            7972..8184
                     /locus_tag="ACHURJ_002816"
     CDS             7972..8184
                     /locus_tag="ACHURJ_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFR1281693.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRG"
     gene            8428..8889
                     /locus_tag="ACHURJ_002817"
     CDS             8428..8889
                     /locus_tag="ACHURJ_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFR1281694.1"
                     /translation="MRKYIPLVLFIFSWPVLSADIHGRVVRVLDGDTIEVMDSLKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNIN
                     QFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            8935..9144
                     /gene="hha"
                     /locus_tag="ACHURJ_002818"
     CDS             8935..9144
                     /gene="hha"
                     /locus_tag="ACHURJ_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFR1281695.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            9183..9773
                     /locus_tag="ACHURJ_002819"
     CDS             9183..9773
                     /locus_tag="ACHURJ_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFR1281696.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQTTLLTEREVSFMKGLFRIVDMTRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            10013..10273
                     /locus_tag="ACHURJ_002820"
     CDS             10013..10273
                     /locus_tag="ACHURJ_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFR1281697.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            10497..10571
                     /gene="tap"
                     /locus_tag="ACHURJ_002821"
     CDS             10497..10571
                     /gene="tap"
                     /locus_tag="ACHURJ_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFR1281698.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            10564..11421
                     /gene="repA"
                     /locus_tag="ACHURJ_002822"
     CDS             10564..11421
                     /gene="repA"
                     /locus_tag="ACHURJ_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFR1281699.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            complement(12473..>12775)
                     /locus_tag="ACHURJ_002823"
     CDS             complement(12473..>12775)
                     /locus_tag="ACHURJ_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR1281700.1"
                     /translation="GQRYRLVFQFPIREQAAAFLGVVPVEKYSGTSVRGCPKLSKIGP
                     PEIRAKLYLASLCGLRFNPLMKAMYERLCLRGKAKMCAIGALMRKLVHWCYGVLKT"
BASE COUNT         3102 a         3220 c         3788 g         2665 t
ORIGIN      
        1 aggaacgacc gaaggtcgct ccggagttta ttccgcgtga catcaacccg gaaatggaga
       61 accgtctgag tgccgtactt gccgcaaggg aagcagaagg tcgtcagatg gccagcctct
      121 tcgaaccgga tgtcccggag gttgtttccg gagaagacgt gactcaggct gaacagccgc
      181 aacagccggt gtctcctgtc atcaacgata agaagtcaga ttcaggtgtg aatgttccgg
      241 caggggggat cgagcaggag ctgaaaatga aaccggaaga agagatggaa cagcaactgc
      301 cacccgggat cagtgaatcc ggtgaagtgg tggatatggc cgcttatgag gcatggcagc
      361 aggaaaatca tccggacacc tggcagcaga tgcagcgtcg tgaagaggtg aacatcaatg
      421 tgcaccggga acgcggggag gatgttgagc cgggagatga tttctgatga acaggtcagc
      481 tccagtcttc agttctcagg ccgcccacac gttcaaattc ccgggtgtta ttagtcacaa
      541 taatcagccc ccgactgcgg gcatgacctg cgatcatttg atcaaatggc ccgacagggc
      601 gtccctgacg ggcaagttct gctcttatct ggccggtgtg tgtggctgca gcagcgtcgt
      661 aatccagaac atcaatgcgg gaaacaaatc cctcgatcac agcgagatta cgttcaggca
      721 tctggctttt ttctgcacca tatatcagct ccatcagggt gaccgaactg atacacattc
      781 tcccctggtt caggttaaaa cgctccctga cgctggcggg tttgttcttt atcgtaaaaa
      841 tgcagatgtt ggtatcgagc ataaacttca gcatcagaat gactcccttt cctgcatgcc
      901 gggctgttcc ctgttatcca taaaatcggc gctgacgctg tgtccgtcga accattcgtc
      961 ccatgtctct ccggctggcg taatgattct ggttcgtccg acagcaatca cttcaacgcg
     1021 ttttacgttt tccggcaatg caaccgcttt tggcagtctg accgcctggc tgcggttgct
     1081 gagaaatacg gtggtttcca ttatttgcct ccttatgtgg atatgtcttg tgtatatcca
     1141 ttgtgggatt gatgccggga tatgtcaaag ggatatacgt ttatgatgag tatcgcgcag
     1201 gtcagatcgg ccggaagtgc cggtaattat tataccaaca aggataatta ctatgtgctg
     1261 ggcagcatgg gagaacgctg ggccggccag ggggctgaac aactggggct gcagggcagt
     1321 gtcgataagg atgtctttac ccgccttctg gagggcaggc tgccggacgg ggcggattta
     1381 agccgcatgc aggatggcag taacaggcat cgtcccggct acgacctgac cttctccgct
     1441 cccaaaagtg tctccatgat ggccatgctc gggggggaca aacgtcttat tgaagcacat
     1501 aaccaggccg tggattttgc tgtccgtcat cacccgtgtg gaggcactgg cctccacacg
     1561 gatgatgacg gacggacagt cagaaacggt gctgaccggt aatctggtga tggcactgtt
     1621 taaccacgac accagtcgcg atcaggaacc acagttacac acgcatgcgg tggtgactaa
     1681 tgttacgcag cataacggcg aatggaagac actgagcagt gacaaagtgg ggaaaacggg
     1741 gttcattgag aatgtgtacg ctaatcagat tgcctttggc aggctctacc gggaaaaact
     1801 gaaagagcag gttgaggcgc tgggctatga aactgaagtg gtcggtaagc acggtatgtg
     1861 ggaaatgccg ggtgtaccgg tggaggcctt ttccggacgc tcacagacta tccgggaggc
     1921 cgtcggggaa gacgcctcgc tgaaatcccg ggatgtggcg gcgctggata cgcgtaaatc
     1981 caaacagcac gtcgatcctg aagtcagaat ggccgagtgg atgcagacgc tgaaggaaac
     2041 cgggttcgac atccgggcat atcgtgacgc agcggaacaa cgggcatata cccgcacgca
     2101 gacacccgga cccgcttcac aggacgggcc ggatgtgcag caggcggtga cacaggccat
     2161 tgccggatta agtgaacgca aagtgcagtt tatgtatacg gacctgctgg ccaggacggt
     2221 cggcatactg ccgccggaaa atggtgtgat tgaacgggca cgcgccggta tcgatgaggc
     2281 catcagccgt gagcagctta tccccctcga tcgtgagaag gggctgttca cgtccgggat
     2341 tcatatgctc gatgagctgt cagtccgggc actcagtcgt gacatcatga aacagaaccg
     2401 ggtgaccgta catccggaga aaagtgtccc ccggacggcc ggttacagct atgccgtcag
     2461 tgttctggca caggaccgtc cgtcgctggc cattgtgtcc gggcagggcg gtgcagccgg
     2521 gcagcgtgag cgggtggctg aactggtcat gatggcccgg gagcaggggc gggaggtgca
     2581 gattatcgct gctgaccgtc gctcgcagat gaacctgaag caggatgaac ggctgtccgg
     2641 tgaactgata accggacgtc gtcagttgct ggaaggcatg gccttcacgc cgggcagtac
     2701 cgttatcgtc gaccagggcg aaaaactctc cctgaaagag acgttaaccc tgctggatgg
     2761 tgccgcacgt cataacgtac aggtcctgat aactgacagc gggcagcgaa ccggtacagg
     2821 cagtgcactg atggccatga aggatgccgg ggtgaacaca tatcgctggc aggggggaga
     2881 acagcgaccg gccaccatca tcagtgaacc ggaccgttat gtccgctatg cccggctggc
     2941 cggagatttt gcggccagcg tgaaagccgg agaagagagc gtggcacagg tcagcggagt
     3001 acgggaacag gccatactga cacaggccat tcgcagtgag ctgaaaacac agggcgtgct
     3061 cggacacccg gaggtaacca tgaccgccct gtcaccggtc tggctggaca gccggagccg
     3121 ttatctgcgg gatatgtacc gcccgggaat ggtgatggag cagtggaacc cggagacacg
     3181 cagtcatgac cgctatgtga cagaacgtgt gacggcgcag agtcacagcc tgaccctgcg
     3241 gaatgcgcag ggcgaaacgc aggtggtgcg tatttcttcc ctggacagca gctggtcgct
     3301 gttccggccg gaaaaaatgc cggtggcaga cggtgagcgt ctgagggtga cagggaaaat
     3361 tcccggactc cgcgtctccg gcggtgaccg cctgcaggtg gcatccgtca gtgaagatgc
     3421 gatgacggtt gttgtgccgg gacgggctga accggccacc ctgcctgtga gcgattcacc
     3481 gttcacggca ctgaagctgg agaacggctg ggtggaaacg cccgggcatt ccgtcagcga
     3541 cagtgcgacg gtttttgcct ccgtcacaca gatggcaatg gacaatgcca ccctgaacgg
     3601 tctggcccgc agtggtcgtg atgtccggct gtattcctca ctggatgaaa cccgtactgc
     3661 ggaaaaactt gcccgccatc cttcctttac ggtggtttct gagcagataa aggcgcgtgc
     3721 cggtgagaca ttgctggaaa ccgctatcag tctgcagaaa gccgggcttc acacgccggc
     3781 acagcaggcc attcatctgg cccttcctgt gctggaaagt aaaaacctgg ccttcagcat
     3841 ggtggacctg ctgacagagg cgaagtcgtt tgctgcagaa ggaaccggtt ttgctgacct
     3901 gggaggggaa atcaatgcgc agataaaacg gggtgattta ctgtatgtgg atgtggcaaa
     3961 aggctatggc acaggcctgc tggtttcccg tgcgtcgtat gaggcagaaa agagcattct
     4021 tcgccatatt ctcgaaggta aggaggcggt cacgccgctg atggagagag tacctggcga
     4081 actcatggag aaactgacat caggacagcg cgccgccgcc cgtatgatac tggaaacgtc
     4141 cgaccgtttc acggtggtgc agggctatgc cggtgtgggt aagaccacac agttccgggc
     4201 ggtgatgtca gccgtgaaca tgctgccgga gagtgaacgt ccccgtgtcg tggggctggg
     4261 gcccacacac cgtgcggtcg gggagatgcg cagcgccggc gtggatgcac agacactggc
     4321 gtcctttctg catgacacgc agctgcagca gcgcagcgga gaaacgccgg atttcagcaa
     4381 cacgctgttc cttctcgatg agagctctat ggtgggcaat accgacatgg cacgggcata
     4441 cgccctgatt gcggccggtg gcggtcgggc tgtggccagc ggtgacacgg accagctgca
     4501 ggccatcgcg cccggtcagc ctttccgtct ccagcagacg cgcagtgctg ccgatgtggt
     4561 catcatgaag gagattgtgc gtcagacgcc ggaactgcgg gaggcggtat acagcctgat
     4621 taaccgggat gtggaaaggg cgctgtccgg gcttgagcgt gtgaaaccgt ctcaggtgcc
     4681 acgtctggag ggcgcatggg caccggagca ctccgtgacg gagttcagtc acagccagga
     4741 agcgaaactg gcagaagcgc agcagaaggc gatgctgaaa ggcgaggctt ttccggatgt
     4801 ccccatgaca ctgtatgcag ccattgtccg cgactatacc ggcaggacac cggaagcacg
     4861 ggagcagacg ctgattgtca cgcacctgaa tgaggaccgg cgcgtactga acagcatgat
     4921 tcatgatgca cgggaaaagg ccggagaact ggggcaggtg caggtcatgg tgcctgtcct
     4981 gaacacagcg aatatacgcg acggggagct gcgtcgtctc tccacctggg agaataaccc
     5041 ggatgccctt gccctggtgg ataatgtgta tcaccggatt gccggtatca gcaaggatga
     5101 cgggctgata accctgcagg atgcggaagg taacacgcgg ctgatttcgc cccgggaggc
     5161 ggtggctgaa ggcgtcacgc tgtacacccc ggacaccatc cgggtgggga ccggtgacag
     5221 gatacgcttc acgaagagtg accgggagcg cggttatgtg gccaacagcg tctggacggt
     5281 gacagcggtt tccggtgaca gtgtcacact ttctgacggt cagcagaccc gtgtgattcg
     5341 ccccggtcag gagcgggcag agcaacatat tgacctggcc tatgccatca ccgcccacgg
     5401 tgcacagggg gcgagtgaaa cctttgccat cgcgcttgaa ggcacggaag gtaaccggaa
     5461 actgatggcc ggctttgagt cagcctacgt ggccctgtcg cgtatgaagc agcatgtgca
     5521 ggtgtacacc gataaccgtc agggctggac ggatgccatc aacaatgccg tacagaaagg
     5581 aactgcccac gatgtgtttg agccgaaacc ggaccgggag gtcatgaatg cagagcggct
     5641 gttcagtacg gcgcgggagc tgcgggacgt ggcggcaggc cgtgccgttc tccgtcaggc
     5701 ggggctggcc gggggagaca gtcctgcacg gtttattgct ccgggacgta aatatccgca
     5761 accgtatgtg gcactgccgg cgtttgaccg taacggcaag tccgccggta tctggctgaa
     5821 cccgctgacc acggatgacg gaaacgggct gcggggattc agcggtgaag gtcgggtaaa
     5881 aggcagcggg gatgcgcagt tcgtggctct gcagggcagc cgtaacggag aaagcctgct
     5941 ggctgataat atgcaggatg gcgtccggat tgcccgtgat aatcctgaca gtggtgtggt
     6001 ggtaagaatc gccggtgaag gccgtccgtg gaatcccggt gccatcaccg gtggccgcgt
     6061 gtggggggat atcccggaca acagcgtcca gccgggagcc ggaaatggcg aaccggtcac
     6121 ggcagaggta ctggcacagc ggcaggctga agaggccatc cgccgtgaaa cggaacgtcg
     6181 cgcagatgaa attgtccgta aaatggcaga gaacaaacct gacctgccgg acggcaaaac
     6241 agagcaggct gtcagggaaa ttgccgggca ggagcgtgac cgggctgcca taactgaacg
     6301 ggaagccgcg ctgccggaga gtgtactgcg tgaaccacaa cgggtgcggg aagcggtccg
     6361 ggaagttgcc cgggaaaatt tgctgcagga gcgtctgcag cagatggagc gggatatggt
     6421 tcgtgacctg cagaaagaga aaaccccggg cggagactga tacaggaaga caaacactga
     6481 tgacaacgga taacacgaac acgacacgta acgattcact ggctgcacgg accgatacct
     6541 ggttgcagtc atttctggtc tggtcacccg gacagcggga tatcatcaaa acggtggcac
     6601 tggtgctgat ggtactggac cacatcaatc tgatattcca gctgaagcag gaatggatgt
     6661 ttctggccgg gcgaggagcc tttccgcttt ttgccctggt gtgggggctg aatctgtccc
     6721 gtcatgcgca tatccggcaa ccggccatta accggctgtg gggatgggga attattgccc
     6781 agttcgcata ttatctggca ggctttccct ggtatgaggg gaatatcctg tttgcctttg
     6841 cagtggcggc acaggtgctg acgtggtgtg aaacgcgcag tgggtggcgt acagcagcgg
     6901 ccatactcct gatggccctg tgggggcctt tgtccggcac cagttacggt attgccgggt
     6961 tgctgatgct ggcagtcagc aaccggctgt accgggcgga agacagagcg gaacgtctgg
     7021 cgctggtcgc ctgcctgctg gctgttattc cggcgcttaa ccttgccacc agtgatgcag
     7081 cggcggtggc cggtcttgtg atgacggtgc tgaccgttgg tctggtgttg tgtgccggaa
     7141 aatcattacc ccgtttctgg cccggagact ttttcccgac gttctatgcc tgtcatctgg
     7201 ctgtgctggg cgttctggcg ctgtgacggg tgtggtatct ttggccgcaa gaggatgatt
     7261 cgtcagaggc agaacacagc atgacagagc agaagcgacc ggtactgaca ctgaagcgga
     7321 agacagaggg aacagcgact gtccgcagcc ggaaaaccat catcaatgtc accacgccac
     7381 caaagtggaa ggtgaaaaag cagaagctgg ccgagaaagc ggcccgtgaa gcagagctgg
     7441 cggcaaaaaa agcgcaggcc agacaggcgc tgtccattta tctgaacctg cccacgctgg
     7501 atgaggctgt gaataccctg aagccctggt ggccgggatt atttgacggt gacacgcccc
     7561 ggcttctggc ctgcggtatc cgggacgtgt tactggaaga cgtggcgcag cggaatatcc
     7621 cgctctcgca taaaaaactg cgcagggcgc tgaaggccat cacccgttca gaaagctatc
     7681 tgtgtgccat gaaagccggt gcctgccggt atgacacgga agggtatgtg acggagcata
     7741 tttctcagga ggaggaagcg tatgcgggag cgcgtctggc gaaaattcgt cgccagaacc
     7801 ggataaaggc agaacttcag gccgtgcttg atgagaaata aaaaaagcct cccccagaga
     7861 gaggaggcag ggaaataagg cttaaaagga aataacttcc gcttccgaaa ggaagccagt
     7921 ccactgaatt atttatcaca ataatactga caactaaagg gggacaagaa aatgaatgga
     7981 ttcagaaaca gttcacggaa cggccaggtc tggcgttacc agcgtgccgg cgggcgggcc
     8041 gttattctgg aagtcagtgg acgctggatg gaagcggcag aagcatggcg acgggctgcc
     8101 tgtgttgcgc ctaggacaga ctggcagcag tttgcccgga aaagggctga acactgccac
     8161 cggcgctgcc ggggaagggg gtaacgaaaa aagcagcctt cattactcca ggagtcagaa
     8221 ggctgaaagg agtttatgag atttgcttct taatatccgg tcgggcagta tgaccggaca
     8281 gttctgagta gcacagagtc ttttccgcat taagtcgatt ttttatattt tgtgattaat
     8341 ttcacaaaat aaggtgttat tcaatgtgtg ctgcactatt caggggggct gaaatacagg
     8401 tgccatttat ctgatatgga gaataacatg aggaaatata ttccactggt attatttatc
     8461 ttttcatggc cggtattgtc tgcagatatt catggacggg ttgttcgtgt actggacggt
     8521 gataccatcg aggtcatgga ctcactgaag gcggtgcgga tcaggctggt aaatattgat
     8581 gcaccggaaa agaaacagga ttatggccgg tggtcaacag acatgatgaa atcactggtt
     8641 gcaggaaaaa cggttaccgt cacatatttt cagcgagatc gttatggtcg tattctcggg
     8701 caggtttatg caccagacgg aatgaatatt aatcagttta tggtccgggc cggggctgcc
     8761 tgggtatatg agcagtacaa cacagatccc gtgctacctg tccttcagaa cgaagccaga
     8821 caacaaaaac gtgggctctg gtcagatgct gatccggttc ccccctggat atggaggcac
     8881 cgtaaataaa gcgatggcaa tcctatcatt catctgttct cagcgggaat aaaaatggca
     8941 aaaacaaaac aggaatggct gtatcagtta cggcgatgtt catcagtcaa tacgctggaa
     9001 aaaattattc ataaaaacag agacagttta tcaaacagtg aacgtgaatc atttaattca
     9061 gcagcagacc accggctagc ggaattaatc accggaaagt tatatgacag gataccaaaa
     9121 gagatatgga aatatgtaag gtaataattc agcaataatt aattgccagg agcagataaa
     9181 taatgaagtt gattattttt attctcattg ttctgattat tgcagcgcta cttatccgga
     9241 ttatcctcag aagtgtaaat cagcattctc ctttgctgat gcaattgcat gcggcaggta
     9301 tacggaccgg tgatgcagag cgaatactgt ccagcggtga atactggcaa cgtcagacga
     9361 cgctgctgac agaaagggaa gtcagtttta tgaaaggact gttcagaatt gtggatatga
     9421 cgcggtggta tctgtgtccg caggtacggg tcgcggatat cgtccagctg aacgggaata
     9481 tccggccacg atcacgccag tggtggcagt tattcaggat ggtgtctcag tggcatgttg
     9541 atgtggttat cgttgagctg cgttcgttca gtattgttgc agcagtagag ctggatgatg
     9601 ccagccattt acgaccggaa cgcagacgcc gggatattct tctggaagag gttctgaggc
     9661 aggctggtat tccgttgctc agaagccacg atgccagaaa actgctgcag atgaccggag
     9721 aatggctgaa tacaacaggg gctgatcagc agtcaccgga acatcgtagc tgacgccttc
     9781 acgttgctca gttgtccaac cccggaaacg ggaaaaagca agttttcctc gctcccggcg
     9841 tttccataac tgaaaaccat actatttcac agtttaaatc acattaaacg acagtaatcc
     9901 ccgttgattt gtgcgccaac acagatcttc gccacaattc tcaagtcgct gatttcaaaa
     9961 aactgtagta tcctctgcga aacgatccct gtttgagtat tgaggaggcg agatgtcgca
    10021 gacagaaaat gcagtgactt cctcattgag tcaaaagcgg tttgtgcgca gaggtaagcc
    10081 tatgactgac tctgagaaac aaatggccgc tgttgcaaga aaacgtctta cacacaaaga
    10141 gataaaagtt tttgtcaaaa atcctctgaa agatctcatg gttgagtact gcgagagaga
    10201 ggggataaca caggctcagt tcgttgagaa aatcatcaaa gatgaactgc agagactgga
    10261 tatactaaag taaagacttt actttgtggc gtagcatgct agattactga tcgtttaagg
    10321 aattttatgg ctggccacgc cataaggtgg cagggaactg gttctgatgt agatgtacag
    10381 gagccagaaa agcaaaaacc ccgataatct tctttaactt tggcgagtca gaaagattac
    10441 cggggcccac ttaaaccgta tagccaacaa tcaagctatg cggggagtat agttatatgc
    10501 ccggaaaagt tcaagacttc tttctgtgct cgctccttct gcgcattgta agtgcaggat
    10561 ggtgtgactg atcttcaaca aacttattac cgccaggtaa agaacccgaa tccggtgttc
    10621 acaccccgtg aaggtgcccg gacgcttccg ttctgcggga aactgatgga aaaggcggtg
    10681 ggcttcacct cccgttttga tttcgccatt catgtggcac atgcccgttc gcttggtctg
    10741 cgtcggcgca tgccaccggt actacgtcgc cgggccattg atgcgctgct gcaggggctg
    10801 tgttttcact atgacccact ggccaaccgc gtccagtgtt ccatcaccac gctggccatt
    10861 gagtgcggac tggcgacgga gtctgctgcc ggaaaactct ccatcacccg ggccacccgg
    10921 gccctgacgt tccttgcaga gctgggactg attacctacc agacggaata tgacccgctt
    10981 atcgggtgct acattccgac cgatatcacg ttcacatctg cactgtttgc tgccctcgat
    11041 gtatcagagg aggcagtggc cgccgcgcgc cgcagtcgtg tggaatggga aaacagacag
    11101 cgcaaaaagc aggggctgga taccctgggt atggatgaac tgatggcgaa agcctggcgt
    11161 tttgttcgtg agcgtttccg cagttaccag acagagctta agtcccgtgg aatgaagcgt
    11221 gcccgtgcgc gtcgtgatgc ggacaggcag cgtcaggata ttgtcaccct ggtgaaacgg
    11281 caactgacgc gtgaaatctc ggaagggcgc ttcactgcca gtcgtgaggc ggtaaaacgt
    11341 gaagtggagc gtcgtgtgaa ggagcgcatg attctgtcac gtaaccgtaa ttacagccgg
    11401 ctggccacag cttccccctg aaagtgacct cctcagaata atccggcacg cgccggaggc
    11461 attcgcacgc ctgaagcccg ccagtgcaca aaaaatcagc gctacataca aaaaacaacc
    11521 tcatcatcca ccttctggtg catccggtcc cccctgtttt tgatacaaaa cacgcctcac
    11581 agacggggaa ttttgcttat ccacattaaa ctgcaaggga cttcaccata aggttacaac
    11641 cgttcacgtc ataaagcgcc agccgtcagc cctacagggt gcaatgtatc ttttaaacac
    11701 ctgtttatat ctcctttaaa ctacttaatt acattcattt aaaaagaaaa cctgttcact
    11761 gcctgtctgt ggacagacag atatgcacct cccaccacaa gcggcgggcc ccgaccgaag
    11821 ccggtttagt tacaacatgc aaacataaac ccctaaaaaa cccaagtccg acgcagaact
    11881 gaaaccacaa agcccctccc tcataactga aaagcggccc cgccccggcc cgaagggccg
    11941 gaacagagtc gcttttaatt atgaatgttg taactatatt atcatcgctg tcagtcctct
    12001 ggctggacgt atcgagtaca cgctcgtaag cggccctcac ggccctcacg gcccgctaac
    12061 gcggagatac gccccgactg cgggtaaacc cttgtcgggc tccgaccgcg cacagaagct
    12121 ctgtcatagc tgaaaacggg tatggcttgg cagctaccat gttctgtcaa gaaaaatcgc
    12181 ttgaactccg acgtacagcc ccctaagtac tgccagcaag ctgtcagcac taagtccccg
    12241 atacagcgcg gcatagccgc gctaacgggg ttaaaagggc ttacctatac ttcatcatcg
    12301 ctgaaagccc gcatggcctc gcagggcaga gatgggatag gtgaaaccta tcaatacgta
    12361 ccggcttacg ccgggcttcg gcggtgttac tcctgtacca tatgtaacta catcatgccg
    12421 ccttacatgc gctacgcgca tatgaagaca aatagcccac atcaaacggt ttttaggtct
    12481 tcagtacgcc atagcaccag tgcacgagtt ttctcatcag cgcaccaatg gcacacattt
    12541 tggccttacc tctcaggcat aatcgctcat acatcgcttt catcaatggg ttaaatctca
    12601 gaccacacaa cgaagccaga tacagtttcg cccggatttc cggcggacca atttttgaca
    12661 gtttcggaca ccctctgacc gaggtgccgg aatatttttc aacgggaacg acacctaaaa
    12721 atgcagccgc ctgttcacgt ataggaaatt gaaaaaccag acgataccgc tgtcc
//
LOCUS       DAZPEB010000119        12733 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZPEB010000119 DAZPEB010000000
VERSION     DAZPEB010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12733
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.120"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..174
                     /gene="flgM"
                     /locus_tag="ACHURJ_002824"
     CDS             <1..174
                     /gene="flgM"
                     /locus_tag="ACHURJ_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFR1281701.1"
                     /translation="VTLSDAQAKLMQPGSSDINLERVEALKLAIRNGELKMDIGKIAD
                     ALINEAQQDLQSN"
     gene            179..595
                     /gene="flgN"
                     /locus_tag="ACHURJ_002825"
     CDS             179..595
                     /gene="flgN"
                     /locus_tag="ACHURJ_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFR1281702.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(635..2170)
                     /gene="murJ"
                     /locus_tag="ACHURJ_002826"
     CDS             complement(635..2170)
                     /gene="murJ"
                     /locus_tag="ACHURJ_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFR1281703.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(2337..3203)
                     /locus_tag="ACHURJ_002827"
     CDS             complement(2337..3203)
                     /locus_tag="ACHURJ_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFR1281704.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGE"
     gene            complement(3205..3852)
                     /locus_tag="ACHURJ_002828"
     CDS             complement(3205..3852)
                     /locus_tag="ACHURJ_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFR1281705.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAELESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(3863..4447)
                     /gene="rimJ"
                     /locus_tag="ACHURJ_002829"
     CDS             complement(3863..4447)
                     /gene="rimJ"
                     /locus_tag="ACHURJ_002829"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFR1281706.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            4683..5885
                     /gene="mdtH"
                     /locus_tag="ACHURJ_002830"
     CDS             4683..5885
                     /gene="mdtH"
                     /locus_tag="ACHURJ_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFR1281707.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHRLM
                     AGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSYMG
                     FSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKRAA
                     RRLLERDA"
     gene            5949..6596
                     /gene="grxB"
                     /locus_tag="ACHURJ_002831"
     CDS             5949..6596
                     /gene="grxB"
                     /locus_tag="ACHURJ_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFR1281708.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            6730..7290
                     /locus_tag="ACHURJ_002832"
     CDS             6730..7290
                     /locus_tag="ACHURJ_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR1281709.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            7396..8442
                     /gene="pyrC"
                     /locus_tag="ACHURJ_002833"
     CDS             7396..8442
                     /gene="pyrC"
                     /locus_tag="ACHURJ_002833"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFR1281710.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVMPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHNFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREAHFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            8516..8761
                     /gene="dinI"
                     /locus_tag="ACHURJ_002834"
     CDS             8516..8761
                     /gene="dinI"
                     /locus_tag="ACHURJ_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFR1281711.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGVTKEDKQRISEILQETWESADDWFVSE"
     gene            9051..9305
                     /gene="bssS"
                     /locus_tag="ACHURJ_002835"
     CDS             9051..9305
                     /gene="bssS"
                     /locus_tag="ACHURJ_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFR1281712.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            9420..10538
                     /gene="solA"
                     /locus_tag="ACHURJ_002836"
     CDS             9420..10538
                     /gene="solA"
                     /locus_tag="ACHURJ_002836"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFR1281713.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            10586..10699
                     /locus_tag="ACHURJ_002837"
     CDS             10586..10699
                     /locus_tag="ACHURJ_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFR1281714.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            10960..11526
                     /locus_tag="ACHURJ_002838"
     CDS             10960..11526
                     /locus_tag="ACHURJ_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFR1281715.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFTLGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            11530..12105
                     /locus_tag="ACHURJ_002839"
     CDS             11530..12105
                     /locus_tag="ACHURJ_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFR1281716.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFEDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(12147..>12733)
                     /locus_tag="ACHURJ_002840"
     CDS             complement(12147..>12733)
                     /locus_tag="ACHURJ_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFR1281717.1"
                     /translation="NHYEYEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYC
                     TGGIRCEKASAWMKHNGFNKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGER
                     ISDEIIAHCHQCGAPCDSHTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPE
                     EQRRRRAGRENGNKIFNKSRGRLNTTLGIPDPTE"
BASE COUNT         3239 a         3288 c         3085 g         3121 t
ORIGIN      
        1 gtgacgttaa gcgacgcgca agcaaaactg atgcaacccg gcagcagtga tatcaatctt
       61 gaacgtgtcg aagcgttaaa actggcgatt cgtaacggtg aactaaaaat ggacatcggc
      121 aaaattgccg atgcgctgat caacgaagct cagcaagact tgcagagtaa ctgatcgtat
      181 gacacgtctt gcagagatcc ttgaccagat gtccgctgtg cttaacgatc tcaaaacggt
      241 aatggatcaa gagcagcaac atctctctat ggggcagatc aacggcagcc agttgcaatg
      301 gattacagaa caaaaaagct cactgctggc gacgctggat tacctcgaac agttacgcaa
      361 gaaagaaccc aatacagcaa atagcgttga tattagtcaa cgctggcagg aaattactgt
      421 gaaaacgcag caactacgcc aaatgaatca acataacggt tggttactgg aaggacagat
      481 tgagcgcaat caacaggcgc tggaaatgtt gaaaccgcat caggaaccga cgctatatgg
      541 ggcgaacggt cagacctcaa caacccatcg cggcggtaaa aagatttcga tctgaagatt
      601 atctccggcc tgcactgcag gccggaatgc attgttacac cgtccggcgg gcaaattctt
      661 taactttgaa gcccagtacc gccagtgcag cgaagtacgc ggcaatcccc gccagcacga
      721 ccgccattaa acgcagtaag cgccagggca tggtacccaa tgaccactcc ggcatgatat
      781 gtaacatacc taaaagcacg ccagacatca ccagtaccgc caccaccaga cgcaacagaa
      841 acgccatcca gccgggttgc ggagtaaaga ttttctgctt acgcaactgc cagtaaagca
      901 gcgaagcatt cagacacgcc gccagaccaa tagaaagtga cagcccggca tgtttcaacg
      961 gaccaataaa cgccaggttc atcaattgcg ttaaaatcag cgtaacgatg gcaattttca
     1021 ctggcgtttt aatgtcctgg cgggaataaa agccaggagc caacactttc actacaatca
     1081 ggccaatcaa acccaccgag taggcaatta acgcccgctg ggtcatcagc gcatcaaacg
     1141 cggtaaattt accgtactgg aacagcgaaa cggttaacgg acctgaaaga atgcccaacg
     1201 caaccgcact cggcagcgcc aacaggaaac aaagacgcaa cccccagtcc atcaaacggt
     1261 tgtattcatc atgattgcca ctggcaaaac ttttcgacag cgacggcagc aaaatggtgc
     1321 caagcgccac gcccagcaca ccggacggaa actccattaa gcggtcggcg taatacatcc
     1381 aagacaccga accggaagca agaaacgagg caaaaatggt gttgatgatt aaggagatct
     1441 ggctcacaga gacgccaagg atcgccggtc ccatctgttt caccacgcgc attgctccgg
     1501 catcgtggaa gttaatgcgc ggcaggacca gcatgccgat cttcttcagg tgcggtagct
     1561 gatacaccag ctgcaggatg ccgccgaccg taacagccca cgccagcgcc agcaccggtg
     1621 ggttaaagta cggtgcggca aacagcgcga aaccaatcat gctgatgtta agcagtgttg
     1681 gcgcaaacgc cggaatcgag aagcggttcc acgtattcag aatcgctccc accagcgacg
     1741 ccagggagat cagcaagata tagggaaagg taatctttag tagctggctg gtcagggcaa
     1801 atttgtcagc tgtatcagcg aagcctggcg cggtcaccat gatcacccac ggtgcggcga
     1861 gcatgccagc gaccgtcaca accgccagcg caagtgtcag caggccagaa acataagaga
     1921 caaagacccg cgtggcgtct tcaccctgct tacttttata ttccgccaga atcggtacaa
     1981 atgcctggga aaatgcccct tcggcaaaga tacggcgtaa caagttagga agtttaaaag
     2041 cgacgaaaaa ggcgtcggtt gccatccctg cgccaaagat tctggcgaca attgcgtctc
     2101 gtgcgaagcc aagcacacgc gaaaacatgg tcatcgagct gacggcggcc agcgatttta
     2161 ataaattcat cggtgttcta atccagacca acgggcaaaa cgcctgcaag acaggcgctg
     2221 aaagaggcga atagtctacc tggattatgg tgaattgcta ccgccagatg ttacagggtt
     2281 tattcactca tcgcatcgcg ccagatcttg tcaacgatac gttgcgccag cacggcctat
     2341 tcgccggcgg tttgcggaac tgtctgattt tgcacacatt caatgaagtg ccgcgcacag
     2401 ccgacaaatc cacgttgttc aagagtgctc tgccaaccag gaatcggttt atgcactacg
     2461 ccctgcccgc gctcctcacg ccattcgcgc atatccgtaa tgtcgatgag cgcaccgtca
     2521 gtcacggcct gcacggtttc acgctgactt ccggcacggc gatgcataca ggtggtgatc
     2581 tgcaaaggac cggccgaaaa atggtgctcg gcaaacagca tttcgccagc gtcgttagtc
     2641 agtagcgtac cgccatccag agaggctttg ccgcccgaca accacagcgc ggtatccacc
     2701 acatgcagat aatcatccag caacgtgaaa taaagatcgt gtggcccgac actattgcta
     2761 cgatgtttat ccattcttag cgaagctgcg gtagcgagtt gcgtttttaa ctcaccgtag
     2821 agtggtgcga aacgacggtt aaaaccgacc atcaacgtca gttttttccg cgccgccagt
     2881 tccaccagcc gttcagcatc gcgcagattt tctgccagcg gtttatcgac acagacatgt
     2941 acacccgcat tgagtaacgt actgaccacg tcaaagtggc tggcggtgct ggaatgcaca
     3001 aaaaccgcat cgcaactggc ggcaaggctg gataacgaat cggcataagg aatgcgccag
     3061 ctttcacaaa ttggcagggc tttcgcgcgc gtaggcgacc aggctccttg taacatccag
     3121 tcagacgctg ccgccagcac cggtaaccac gctttttgcg caatgccgcc taatcccaca
     3181 acgccgatac gtaatttttt cactttaatc tcccagatgg gccagcaacg aatcaagacg
     3241 ctgtttcagt tctgccactt cgatttccag ggcttcgacg cgggcctgta aatcaccatc
     3301 aaccgcgttc gacatatccg ttaccgccgg ctgatcctct acctcaccac tgaaaagatg
     3361 catgtagcgg ctttcgcgtt tacccggttc gcgggccaga cgcaccacaa aaggaccatc
     3421 ttcgcgattt gccagttgtt ccagcgtcga ctccaattcc gccatatcgc tgaattcata
     3481 cattcgcgcg gcgcggctgc gcagttcacc cggcgtctgg gcaccacgca ataacaacgt
     3541 ggtgattaac gccacttccg ctgcgctcag tttcagatcg ccaaattctg aattacaaaa
     3601 acgttgttca tatttggtga cccgattacc aaagccactc accgtgcgca gataatggcg
     3661 tttgaccaga ttatccagtt gctcctgcac ttcagattca ctcagattca tgaccggttc
     3721 acggttcgtt ttctgattac aggccgtgac tacaccattg actgagagcg ggtattgctc
     3781 cggcgtggtc acctgctttt ccagcaagca accaatcacg cgagcttcaa gggcagtaag
     3841 ttgatatttc atgagattct ccttagcggc cgggcgtcca gtctggggta gttaatgccg
     3901 tcagtacgtg atcgcgccat tgtccatcaa tcaacagata gtctttcgca tagccttctt
     3961 tttcaaaacc cagtcgcgcc agtaaatcac cgctgcgttt attgtgcggc atataattag
     4021 ccataatgcg atgaatatgt tgggtgcgct gcatataacg aatggctgcg gtcagggctt
     4081 caaacatgag tcctttgccc tgccattttt gcccaatcga ataaccgaga tagcaggcat
     4141 gaaaagagcc acgaacaaca ttggaaaaat tggcaacgcc aataatctct ttttcgtccg
     4201 ggtcgaataa gccaaagtag aaagctgaac cttgtttatg aaattcgtta atcatcccca
     4261 gcctggcctg ccagcctgat ggataacagt ggctttcgtc gcgcactggc tcccagggct
     4321 tgaggaaatg gcgattctct gcgtaataat ccgcaagacg ccaggcatca cgatcatgca
     4381 ccagacgcac gaccagtcgg tctgtggtta agcgcacttt tggcacgtta ctgcgatagc
     4441 caaacattct atacctactc cttcccgtaa cgtctttaat acgctgtaag tattactata
     4501 cctgcgcaac cagaccctgt gaaaagtcca aaatgccatt ttctgcatta ttcacatatt
     4561 tgataaaaac tctctatcaa agcgacgttt tgataaaaaa atattgtccc cgaagtgatg
     4621 ggaaaagtct tagcggcaat gcagaataga aagtgctcat cttttatttc ccgggagggg
     4681 aaatgtcccg cgtatcgcag gcgaggaacc tgggtaaata tttcctgctc atcgataata
     4741 tgctggtcgt gctggggttc tttgttgtct tcccgctgat ctctatccgc ttcgttgatc
     4801 aaatgggctg ggccgccgtc atggtcggta ttgctctcgg tctacgccag tttattcagc
     4861 aaggtctggg tattttcggc ggcgcaattg ccgaccgctt tggtgccaaa ccgatgattg
     4921 ttaccggtat gctgatgcgc gccgccggat tcgccacaat gggtatcgcc cacgaaccgt
     4981 ggctattgtg gttttcatgc ctgctctcgg gactcggcgg cacgttgttt gatccgccgc
     5041 gttcggcgct ggtggtgaaa ctaatccgtc cacaacaacg tggtcgtttt ttctcgctgt
     5101 tgatgatgca ggacagtgcc ggtgcggtca ttggcgcatt gttggggagc tggctgttgc
     5161 aatacgactt tcgcctggtc tgcgccacag gggccgtttt gtttgtgctg tgtgcggcgt
     5221 tcaatgcgtg gttgttaccg gcatggaaac tctccaccgt acgcacgccc gttcgcgaag
     5281 gcatgacccg cgtgatgcgt gacaagcgtt ttgtcaccta tgtcctgacg ctggcgggtt
     5341 actacatgct ggctgtacaa gtgatgctgc caattatggt caacgacgtg gctggcgcgc
     5401 cctctgccgt taaatggatg tatgccattg aagcgtgtct gtcgttaacg ttgctctacc
     5461 ctatcgcccg ctggagtgaa aagcattttc gtctggaaca ccggttgatg gctgggctgt
     5521 tgataatgtc attaagcatg atgccggtgg gcatggtcag cggcctgcaa caacttttca
     5581 ccctgatttg tctgttttat atcgggtcga tcattgccga gcctgcgcgt gaaaccttaa
     5641 gtgcttcgct ggcggacgca agagctcgcg gcagctatat ggggtttagc cgtctgggtc
     5701 tggcgattgg cggcgctatt ggttatatcg gtggtggctg gctgtttgac ctgggcaaat
     5761 cggcgcacca gccagagctt ccgtggatga tgctgggaat tattggcatc ttcactttcc
     5821 ttgcgctggg ttggcagttt agtcagaaac gcgccgcgcg tcgtttgctg gaacgcgacg
     5881 cctgatttga tactcccccc tgactcctcc atactgaaag tgaactgaca gccaaatgga
     5941 ggagtcacgt gaagctatac atttacgatc actgccctta ctgcctcaaa gcccgcatga
     6001 ttttcggcct gaaaaatatc cccgtcgaat tacatgttct gctcaacgac gacgcagaaa
     6061 cacccacccg gatggtcggt caaaaacagg ttcccattct gcaaaaagat gacagccgct
     6121 atatgccaga aagcatggat atcgttcact atgtcgataa actcgacggc aaaccgttac
     6181 tgaccggcaa acgttcccct gccattgaag agtggctgcg caaggtcaat ggctacgcca
     6241 acaaactgct gttgccgcgt tttgccaaat cggcatttga tgagttttct actcccgccg
     6301 cgcgcaaata tttcgtcgac aagaaagagg ccagcgcggg taattttgcc gacctgctgg
     6361 cccactctga cggtctgatt aagaatatca gcgatgattt gcgtgcgctg gacaaactga
     6421 tcgtcaaacc gaacgccgtg aatggagaac tttcggaaga tgatattcag ctatttccgc
     6481 tactgcgtaa tctgacgctg gtagccggaa tcaactggcc aagccgcgtt gctgattacc
     6541 gcgataatat ggcgaaacag acacaaatca atttgttatc atcaatggcg atttaatcct
     6601 gacccgccgc ggcagcctgt attcgcgggt tgccgccgtt ttcagttttt tctgctggcg
     6661 agtttatcgc tggtgacaca tgctgcgttg tgtcaggata ttgacgcaga tttcatgtcg
     6721 aggactacca tgaacaagtt tttatttgcc gctgcattga tcgtcagtgg cctgctcgtt
     6781 ggctgtaatc aactcaccca atacaccatc accgaacaag aaattaacca gtcgcttgcg
     6841 aaacataata atttctcaaa agatatcggt ttacccggcg tggctgacgc ccatattgtt
     6901 ctgacaaact tgaccagcca aattggtcgc gaagagccga ataaggttac cctaaccgga
     6961 gacgccaatc tggacatgaa ctccctgttc ggtagtcaga aagcgaccat gaaactgaag
     7021 ctgaaagcgc tgcccgtgtt tgataaagaa aaaggtgcga tcttcctgaa agagatggaa
     7081 gtggtcgatg cgacggtaca accggaaaaa atgcaaacgg tgatgcaaac gttgcttccc
     7141 tatttgaacc aggcattacg caattacttt aaccagcaac ctgcttacgt cctgcgcgaa
     7201 gatggcagtc agggcgaagc aatggcgaaa aaactggcga aaggcattga agtgaagcca
     7261 ggcgaaattg tcattccgtt tacggattaa tgacgagggc gcattcgcgc cctttatttt
     7321 tcgtgcaaag gaaaacgttt ccgcttatcc tttgtgtccg gcaaaaacat cccttcagcc
     7381 ggagcataga gattaatgac tgcaccatcc caggtattaa agatccgccg cccagacgac
     7441 tggcaccttc acctccgcga tggcgacatg ttaaaaactg tcatgccgta taccagcgaa
     7501 atttatggac gggctattgt aatgcccaat ctggctccgc ccgtgaccac tgttgaggct
     7561 gccgtggcgt atcgccagcg tattcttgat gccgtacctg ccgggcacaa tttcacccca
     7621 ttgatgacct gttatttaac agattcgctg gatcctaatg agctggagcg cggatttaac
     7681 gaaggcgtgt tcaccgctgc aaaactttat ccggcaaacg caaccactaa ctccagccac
     7741 ggcgtgacgt caattgacgc aatcatgccg gtacttgagc gcatggaaaa aatcggtatg
     7801 ccgctactgg tgcatggtga agtgacacat gcagatatcg acatttttga tcgtgaagcg
     7861 cactttatag aaagcgtgat ggaacctctg cgccagcgcc tgactgcgct gaaagtcgtt
     7921 tttgagcaca tcaccaccaa agatgctgcc gactatgtcc gtgacggaaa tgaacggctg
     7981 gctgccacca tcactccgca gcatctgatg tttaaccgca accatatgct ggttggtggc
     8041 gtgcgtccgc acctgtattg tttacccatc ctcaaacgca atattcacca acaggcattg
     8101 cgtgaactgg tcgccagcgg ttttaatcga gtattcctcg gtacggattc tgcgccacat
     8161 gcacgtcatc gcaaagagag cagttgcggc tgcgcgggct gcttcaacgc cccaaccgcg
     8221 ctgggcagtt acgctaccgt ctttgaagag atgaatgctc tgcagcactt tgaagcattc
     8281 tgttctgtaa acggcccgca gttctatggc ttgccggtca acgacacatt catcgaactg
     8341 gtacgtgaag agcaacaggt tgctgaaagc atcgcactga ctgatgacac cctggtgcca
     8401 ttcctcgccg gggaaacggt acgctggtcc gttaaacaat aaaaatcgta gcttcctgtt
     8461 gtcattaggt tattttacct gtataaataa ccagtatatt caacaggggg ctattatgcg
     8521 aattgaagtc accatagcga aaacttctcc attgccagct ggggctattg acgccctggc
     8581 tggcgaactt tcccgccgta ttcagtatgc gtttcctgat aatgaaggcc acgtatcggt
     8641 acgttatgcc gcagcgaata atttatcggt tattggcgta acaaaagaag ataaacagcg
     8701 cattagcgaa attctccagg aaacgtggga aagcgccgat gactggtttg tcagcgaata
     8761 atatgcagtg atttttgttt ttgccgggtc gccccggctt ttttatttta cttacaaatt
     8821 ttgagtacaa aatttctaca cttcctcaaa ataattgcac aatatgctaa aaaaatatga
     8881 atgacatgct gttttattgt tcatataaat cttaaaagca gaaatttatc tgttcccccc
     8941 tttaaaaatt cggtgaatac ccttacttat tggtatactg aagttgcttc agataaaaac
     9001 gcattgaacc tcgaataacg ttgtctagta acacgaatta gggggccatg atggaaaaaa
     9061 ataatgaagt cattcagact catccgctcg tagggtggga catcagcacc gttgatagct
     9121 atgatgcgct gatgttgcgt ttgcactacc agaccccaaa taagtccgag caggaaggga
     9181 ctgaagttgg tcagacgctc tggttaacca ctgatgttgc cagacaattt atttcgatat
     9241 tagaagcagg aatcgccaaa attgaatccg gtgatttccc ggtaaacgag tatcggcgtc
     9301 attaacatga catgaccatc cacccaaaag gcacctcacc ggtgccttta ccatttctgg
     9361 gttgtatatc tgcattcagt taattactct gcttaaacca tgagagcaga gagaaaaaaa
     9421 tgaaatacga tctcatcatt attggcagcg gttccgtagg cgctgccgcc gggtattatg
     9481 caacccgcgc cggtttaaac gtgctaatga ccgacgccca tatgccaccg catcaacacg
     9541 gcagccacca cggcgatacg cgattaattc gccatgctta tggtgaaggc gaaaagtatg
     9601 tcccgttggt cctccgcgcg caaacgctgt gggatgaact ttcccgccac aacgaagatg
     9661 atcccatttt tgtacgctct ggtgtcatta acctcggccc ggctgactcc gcatttctcg
     9721 ccaacgtcgc ccacagcgcc gaacagtggc aactcaacgt tgaaaagctc gatgcgcaag
     9781 ggattatggc ccgctggcca gaaatacgcg tcccggacaa ctacatcggc ttatttgaga
     9841 ctgattccgg ttttttgcgc agcgaactgg cgattaaaac ctggatccaa ctggcgaagg
     9901 aagcgggctg tgcgcaactg ttcaactgcc cggtcaccgc aattcgtcat gacgatgatg
     9961 gcgtaactat tgaaaccgtt gacggtgagt atcaggcgaa aaaagcgatt gtctgcgcgg
    10021 gaacatgggt aaaagacctg ctcccggagc tgcctgtcca gcctgtacgt aaagtatttg
    10081 cctggtatca ggccgatggc cgctatagcg tgaagaataa attcccggcg tttaccggtg
    10141 aactgcccaa tggcgatcaa tattatggtt ttccggcaga aaacgacgcg ttgaagattg
    10201 gcaaacataa cggaggccag gttatccatt cagcggatga acgtgttccg tttgcggaag
    10261 tggtcagcga tggttcggaa gccttcccgt tcttgcgcaa tgtattgccg ggtatcggtt
    10321 gctgcctgta cggcgctgcc tgcacctatg ataattcgcc tgacgaagat tttattatcg
    10381 ataccctacc cggccacgat aatacactgc tcattaccgg cctgagtggg cacggtttta
    10441 aatttgcgtc agttttaggg gaaatagctg ccgattttgc gcaagacaaa aaaagcgatt
    10501 ttgatttgac gccattcagg ctttcccgct tccaataatc atcattacgg cctccagaat
    10561 gaggccgttt ttacaggagt atttaatgcg tcggctgttg cactatctca tcaataatat
    10621 tcgcgagcat ctgatgcttt atctttttct ctggggattg ttggcaatta tggatctgat
    10681 ctatgtgttt tatttttagt tttccgcact gacataaact gacaaatatt taatctccct
    10741 ttcatttagc gtgaaaaatt gaatgagatt attcctgctt ctacagcact caccagacat
    10801 ctctgatgct gaacccacta aacttatgaa gattctttca ttattaattc aaaattattg
    10861 aaataaaaat aaatatcatt caatttgtta tttttgtgtt gcaattcatt ctccgacacg
    10921 ccattttctt tctctcgcaa attcgtattg gatgcactta tgtcattcac aaatacccct
    10981 gaacgctatg gtgttatatc tgcggccttc cattggctaa gcgcaattat tgtctacggc
    11041 atgtttactt taggtctgtg gatggtcacg ctcagttatt acgatggctg gtatcacaaa
    11101 gcacccgaac tgcataaaag catcggtatt ttgttaatga tggggctggt tattcgcgtt
    11161 ctgtggcgtg tcatatctcc accgccgggt ccgctgccga gttattcgcc catgactcgt
    11221 cttgccgcaa aggctggaca tctcgcattg tacctattgc tgttcgccat cggcatcagc
    11281 ggctatctga tctcaactgc cgatggtaaa ccgatcagcg tttttggctg gtttgacgtc
    11341 cccgcgaccc ttgccgacgc tggcgcacag gcagactttg ccggtgccct gcatttttgg
    11401 ctcgcgtgga gcgtcgtagt actgtccgtt atgcacggat ttatggccct gaagcatcat
    11461 ttcatcgata aagacgacac tctgaagcgc atgctgggaa agtcgtcatc tgactatgga
    11521 gtataaaaaa tgaaaaaaag cctgcttggt ttaaccttcg cgtccctgat gttctctgcc
    11581 ggttcagcgg ttgccgccga ttacaaaatt gacaaagaag gtcagcacgc ctttgttaat
    11641 ttccgcatcc agcaccttgg ctatagctgg ttatacggca cctttgaaga tttcgacggt
    11701 acttttacct ttgacgaaaa aaatccggct gccgataaag tgaatgtgac aattaacacc
    11761 accagcgtcg atactaatca cgccgaacgc gataaacatc ttcgcagtgc agatttcctc
    11821 aataccgcaa aatatccaca ggcaacattc acctccacca gcgtgaagaa agacggtgac
    11881 gaactggata ttaccggcga tctgacgctg aatggcgtaa ccaaacccgt cacgctggaa
    11941 gcgaaattaa ttggtcaggg cgacgaccca tggggtggta aacgtgcagg cttcgaggcc
    12001 gaaggcaaaa ttaagctcaa ggacttcaat atcaagacag atttaggtcc agcttctcag
    12061 gaagtggatc tgattatttc agtggaaggc gtacagcaga agtaagcgtg aaatgttgcc
    12121 ggatggcacg catccggcaa tgatatttat tctgttggat caggaatgcc cagtgttgta
    12181 ttcagacgtc cacgagactt attaaagatc ttattgccat tttcacgtcc tgcccgacgg
    12241 cgtcgctgtt cttctggcgg taacgcgctt tcttcgcagc aaatctcact acaacaacct
    12301 ttgtattttt ccgcgcatac tggacactga ataaacagca ggtggcagcc atcattttta
    12361 cagttggtat ggctgtcgca cggcgcgccg cactggtggc aatgcgcgat aatctcatcc
    12421 gatatacgtt cgcccatccg ctcgtcaaaa acaaaatttt tgccaataaa acgcaccggc
    12481 aagccctgct cgcgcgcctt acgggcgtat tcaataattc cgccctcgat atgccagact
    12541 ttgttgaatc cgttatgttt catccaagca ctggcctttt cacaacgaat gccgccggtg
    12601 cagtacatga cgattttttt atctttatgt gcctgcatca tctcgactgc ttttggcagc
    12661 tgctcacgga aggtatctgc cggaatttcg agcgcgtttt caaagtgccc cacttcatac
    12721 tcatagtggt tac
//
LOCUS       DAZPEB010000120        12660 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZPEB010000120 DAZPEB010000000
VERSION     DAZPEB010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12660
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.121"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(4..324)
                     /gene="chbB"
                     /locus_tag="ACHURJ_002841"
     CDS             complement(4..324)
                     /gene="chbB"
                     /locus_tag="ACHURJ_002841"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFR1281718.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(622..960)
                     /gene="osmE"
                     /locus_tag="ACHURJ_002842"
     CDS             complement(622..960)
                     /gene="osmE"
                     /locus_tag="ACHURJ_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFR1281719.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            1162..1989
                     /gene="nadE"
                     /locus_tag="ACHURJ_002843"
     CDS             1162..1989
                     /gene="nadE"
                     /locus_tag="ACHURJ_002843"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFR1281720.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALACPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPQQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            2219..3106
                     /gene="cho"
                     /locus_tag="ACHURJ_002844"
     CDS             2219..3106
                     /gene="cho"
                     /locus_tag="ACHURJ_002844"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFR1281721.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRTALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEDATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(3066..3641)
                     /gene="ves"
                     /locus_tag="ACHURJ_002845"
     CDS             complement(3066..3641)
                     /gene="ves"
                     /locus_tag="ACHURJ_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFR1281722.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFAADQVVKAKLTEGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(3844..4314)
                     /gene="spy"
                     /locus_tag="ACHURJ_002846"
     CDS             complement(3844..4314)
                     /gene="spy"
                     /locus_tag="ACHURJ_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFR1281723.1"
                     /translation="MRKLTALFVASTLALGAANLAHAAAPADAKPMMHHKGKFGPHQD
                     MMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIVSDTFDKAKAEAQIAK
                     MEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATAE"
     gene            complement(4644..5612)
                     /gene="astE"
                     /locus_tag="ACHURJ_002847"
     CDS             complement(4644..5612)
                     /gene="astE"
                     /locus_tag="ACHURJ_002847"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFR1281724.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGDETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(5605..6948)
                     /gene="astB"
                     /locus_tag="ACHURJ_002848"
     CDS             complement(5605..6948)
                     /gene="astB"
                     /locus_tag="ACHURJ_002848"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFR1281725.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(6945..8423)
                     /gene="astD"
                     /locus_tag="ACHURJ_002849"
     CDS             complement(6945..8423)
                     /gene="astD"
                     /locus_tag="ACHURJ_002849"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFR1281726.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKLRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGRANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQLETSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     ILMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(8420..9454)
                     /gene="astA"
                     /locus_tag="ACHURJ_002850"
     CDS             complement(8420..9454)
                     /gene="astA"
                     /locus_tag="ACHURJ_002850"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFR1281727.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWHNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAVEKTA"
     gene            complement(9451..10671)
                     /gene="astC"
                     /locus_tag="ACHURJ_002851"
     CDS             complement(9451..10671)
                     /gene="astC"
                     /locus_tag="ACHURJ_002851"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFR1281728.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIMAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDATCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGDFPVGALLTTEECASVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            11117..11923
                     /gene="xthA"
                     /locus_tag="ACHURJ_002852"
     CDS             11117..11923
                     /gene="xthA"
                     /locus_tag="ACHURJ_002852"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFR1281729.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            12090..>12660
                     /locus_tag="ACHURJ_002853"
     CDS             12090..>12660
                     /locus_tag="ACHURJ_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFR1281730.1"
                     /translation="MNAERKFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITL"
BASE COUNT         3076 a         3428 c         3099 g         3057 t
ORIGIN      
        1 aaattaattt gctgcggctt ttttaatcgc tgcaaccgca gccttaagca cgcctaaacc
       61 atcgactttg ccataaagca gcgagtcaat tacttcaacc ggtttgttgg gtaacaaacg
      121 ctggatttcg ggcaacatat aagcaatctg cggccctaat aacacgacat cggcattctg
      181 acctttttca ccagccagtg tttccggaaa tgcttcaata atgaccggaa cttcatattt
      241 ttctgcctgt gcgcgcattt ttgataccag taaagaggta gacatgcccg cagaacaaaa
      301 cagataaatg tgtttctttt ccataaaact gccctcatcg acgattatct gtcagccaga
      361 cactccgcaa gccttaacct gcttccatgc tctgggtaac ttgcgaaacc aacatgactt
      421 tttttgtggc tgaaaggagt atacggtata gacctgagca atagtatttc tttgacctta
      481 taaattgtct ctccttgacc tgcggttatg accctcttca catttcgggc aaataattcg
      541 cgaagataat taagaactca ggccaaaaaa accggcgcaa tggccggttt ccgttgttac
      601 tcaagctttc aaacgaagtg attacttcgc agcctgtgga tcagtgtcgt attcagcaca
      661 ggtctgataa ccggagttga tgacatgtcc ggtatcatct aacgcgacaa agtaggtttc
      721 tgctttacca tcacgttgac ccaggatgta ggtctggcaa gtaccacgag catggatcat
      781 gctcacttca gacgaaggtt tacccgcaat ttgtgcaacc tgcgcccggc tcatgccttt
      841 tttgacgtct ttcaccacag gctgtacaaa ctggtctttg gtacgatcat aagccgtaca
      901 acccgccagc atggttaata ccgccgctgc actcagaatt cctgccatat tcttgttcat
      961 attccgtcct cttgtttatc agcgtgttag ataagcctgg aatacattgg gcgctttttc
     1021 aagcccgtga acgaaacggc tccgctttca gaggattcct gtatgacgtt ttatccacca
     1081 ttcaacccgc tgtcgcttgt cgtttcagta gcgacgggtt agctttaagg aagttttgtc
     1141 ttttctgtct ggaggggttc aatgacattg caacaacaaa taataaaggc gctgggcgca
     1201 aaaccgcaga ttaatgctga agaggaaatt cgtcgtagtg tcgattttct gaaaagctac
     1261 ctgcaaactt atccgttcat taaatcactg gtgctcggga tcagcggcgg tcaggactcc
     1321 acgcttgccg gtaagctgtg ccagatggcg attaatgagc tgcgccagga gaccggcaac
     1381 gaatcactgc aatttattgc cgtacgcctg ccctatggtg ttcaggccga cgaacaagat
     1441 tgccaggatg ccattgcctt tattcaaccg gatcgcgtat taaccgttaa tatcaagggc
     1501 gcggtattgg ccagcgaaca ggcgctgcgc gaagcgggca ttgaactgag cgattttgtc
     1561 cgtggcaatg aaaaagcgcg tgagcggatg aaagcacaat atagcattgc gggtatgacc
     1621 agcggtgtcg tggtgggcac cgatcatgca gcagaagcta ttaccggatt cttcactaaa
     1681 tatggtgacg gcggtacgga cattaatccg ctgtatcgtc tcaacaaacg tcagggtaaa
     1741 cagttactgg cggcattagc ttgcccggaa cacctttata agaaagcgcc aacggccgat
     1801 ctggaagatg atcgcccttc tctgccagat gaagtggcac tcggcgtgac ctatgacaat
     1861 atcgacgact atctggaagg gaaaaacgta cctcaacagg tcgccagaac aatagagaac
     1921 tggtatctga aaaccgaaca taaacgccgt ccgccaatta ccgttttcga tgatttctgg
     1981 aaaaagtaat aatttgcaca tattggattg tgcgaaaaag agtaatttgt tcacgccgga
     2041 tgcggcgtga acgccttatt cgacctataa aactatgcaa attcaataca ttgcaggagt
     2101 cgaataggcc tgacaggcgt agcacgtcag acggtgtaac ctttgtcatc gacccgcctc
     2161 ttttttaatc gcttcccgcc tgttacactg gatagataac cagcattcgg agtcaacagt
     2221 ggtacggcgt ttaacttctc cgcggctcga atttgaagct gcggcaattt atgaatatcc
     2281 cgaacattta cgttcattcc ttaatgactt acccacccga ccaggggtgt atctgtttca
     2341 tggtgaaagt gacaccatgc cgctctatat cggcaaaagc gttaacatcc gcagccgcgt
     2401 cctttctcat ttacgtaccc cggatgaagc cgccatgcta cggcaatccc gacggatcag
     2461 ctggatatgt accgccggtg aaatcggcgc tctgctcctt gaagcgcgat taatcaaaga
     2521 acaacagccg ctgtttaata aacggttgcg ccgcaatcgt cagctctgtg ccctgcaatt
     2581 aaatgaaaag cgcgtcgatg tggtgtatgc caaagaggtg gatttttcac gagcccccaa
     2641 cctgtttggc ctgtttgcca ataggcgcac agctttgcaa gcattgcaga ccatcgctga
     2701 tgaacaaaaa ctttgttatg gcctgctggg actggaaccg ttaagtcgcg gtcgtgcatg
     2761 ttttcgttca gcgctaaaac gttgcgccgg agcatgctgc ggtaaagaga gccatgagga
     2821 acatgcgcta cgcttgcgcc agtctctgga gcgtttgcgg gtggtgtgtt ggccttggca
     2881 aggggcggtg gcgctgaaag aacagcaccc ggaaatgact caatatcata ttattcaaaa
     2941 ctggctgtgg ctgggggcgg ttaattcgct ggaagacgcg acaacgttaa ttcggacacc
     3001 cgccgggttt gatcacgacg gttataaaat tctttgtaag ccgctgcttt ccggtaacta
     3061 tgaaattact gaacttgatc cggcgaatga ccagcgagcc agttgatttc actgaacaac
     3121 agtttacctt gcggttgtaa aagccgcagg gtgtgcctgc catcgaacca gcacgcgccc
     3181 tggtcggtag tgagcaattt atcgcccagt tgccacgccc cgttaatcac aaacaccacc
     3241 ccgccgcgtg agccaaaggt agtaaacgta cgttcggcaa ttctgacttt cgccttacag
     3301 acatccaggc gtgtcatgat gttgaaatcc atcgacatct gcccttccgt cagtttcgct
     3361 tttaccacct ggtccgctgc aaaggcaaaa ggctgtaacg gttttaaggt atggttaaag
     3421 cggtctgcgc tttcaaggag catctcgccg ccttccagca acgtcactat cctttccatg
     3481 ccgggaaaca aagaaaactc cccattcgcc gcgatggatg caatgctggc acgccagtaa
     3541 aaatcacgtt ttgccggtgg gaaagtgcaa atttcgcgcg tttcaccggc agcatttcgc
     3601 cacagattca ccgacatttt acgcatatca aagtattcca tactcgctcc cggcccgttc
     3661 ttttcattgt tatcgttcag gtaaccgtgg atggttatcg gcaactcggg tgatttacgt
     3721 tagtggtgat caggcttttt ttacaactgt ttgagaagag aaaagaaaac cgccgatcct
     3781 gtccaccgca ttactgcaag gtagtggaca agaccggcgg tcttaagttt tttggctgaa
     3841 agattattca gcagttgcag gcattttacc ttttgccgct ggacgttctg tcagacgctt
     3901 ctcaaaatta gcattaaatt gctttttctg ttccggcgtc aggatgttgt aaattttgtt
     3961 ctgggtttcc atgtgcgcca gcatgttagc tttgcgctgt tcttccattt ttgcgatctg
     4021 cgcttcagct tttgctttat cgaaggtatc gctgacaatg atgtcatgca ttgcgcggcg
     4081 ttcttccagc ggcggacgtt tcatctggtc acgctggcct ttcatgattt cgcggatctg
     4141 ctgtttctgc gcgtcggtca ggttcaggtc tttgaacatc atgtcctgat gcggaccgaa
     4201 cttgcctttg tggtgcatca tcggcttcgc gtcagccggt gctgcggcat gggccaggtt
     4261 agccgcgcca agagccaggg tagaggcaac aaacagtgca gttaatttac gcatattcta
     4321 tatccttcct ttcagttatt tattacggct ttcttaagta gcgtgccgtg ttgacaagat
     4381 taactttact gactttagcg tcaattaatc agagcaacgg taaaacaatg aaagtgtaaa
     4441 aaacactttt gcgccaatta tggagaaaaa aagaaaattt gatggagagt gatgagagaa
     4501 tattacaaca caatgatttt gcagagatta tgaagaacta tactggatga ctggtgataa
     4561 ataaagcaaa taaccaggat taatctgtat taatttataa gaaagcaact taatacccgc
     4621 agaatgattt ctgcgggtaa gtattagctt attttttcga gcattaatcc cgcgcgtaat
     4681 cccaacgcta ccaacggatt agggaataac acatactcta catcatgggt tacggtaaaa
     4741 cgttcctctc cgtcctgcgc cagcaatgtt cctttctcaa acggcataaa attcagcgtg
     4801 tcacttgcca tatgcatttc gaaggacggc gagtggcgag taatttgcga aaccacccga
     4861 taacggagcg gcggtgttct cacgataccg acactctcac cagatagcag cgcagcaatt
     4921 gcgctggcag ttactgcaaa ctggcgaaga tcgttttgcc caaagggcaa cgctttgcca
     4981 agttccagcg tacaggccag cgcgccaaaa tgtctggcgc tgaaatgggt aaacgtacca
     5041 ccaggttcct gatggaacac cagcgcctcc agccccgccg cacccagcca cgtcagaaat
     5101 ttctcgtccc agggaatgtc gcgttgcggt aatacaccga actgcggatg caaggagcca
     5161 cgaattgcgg tatgtagatc aaggtgccag cgcacagatt ctttgccctg gtcataaaaa
     5221 tcttccaggc actgttccag ttcgcgcgcc cgacaggttt ctccgctttc agcaaatagc
     5281 tgccaacgac cgccaaacat tcgattcata tcgctatggc aataacgttt cccttgcttc
     5341 agcgcaggag gattcccgag gatcaccagc aaccgccaac gtaacgggat ctcgccgtga
     5401 gatatcgcgc caagcaacgc gtccagcatt tccacaggtg ccgtctcatc accgtggatc
     5461 cccgcagaaa tcaccagcgc gccttgcggt ggcgttaatg gcgtcagttc cagcacacca
     5521 tcgcccagcc agcgccagcg aacgccgttg atttctcgct cggtgataac cggtttttta
     5581 cccgttaagg tcagagcaag aaaattatcc attgccccct ccctcgcgct ggaacggata
     5641 aaccgaaccg agattcagta attggctcaa tacatccagt gcttcccgcc cttcgcgcag
     5701 caattgcggg tcggccagat cggcagcagt aagacgatcg cggtagtaac gatccaccca
     5761 gtcattgagc gcattaaaca aggtatcgtt catcatcacc gccggattca ccgcccggcg
     5821 ttcttcttct gtcaatacca cccgcaaccg caggcacgcc gggccgccgc cattcgccat
     5881 gctttcacgt aagtcaaaga cttttagttc gctaatcggg ttgtcagcgg caaggagttc
     5941 attgagataa ccccatactc cggcgtgttc ccgacactcc tgaggcagca ccaacatcat
     6001 ggaaccatca tcgcggctca acagttggct gttaaacaga taggtagaca ccgcatccga
     6061 cacggaaacc tgagttgccg gaacttctat cgccataaaa ccgttcaccc gcgcccgcag
     6121 gtttgccagt aactgtgact ggcgagcgaa cgcctgttgg tggcaaaaca gcacctggcg
     6181 gttacttacg gcaatcacgt cattatgaaa aacaccctgg tcgataacgt ccgggttttg
     6241 ctgggcgaaa atcacctgtt ggggattcac ctgattaagc cttgccaccg cctcgctggc
     6301 ttcgcgagtc tgtcgcgccg gataacggga aggccgggta tcattgcctt cttctcgccc
     6361 gtagacaaaa agttgcatac ccggctcacc gtaatgaccg ccgagacgat tgtggtttgc
     6421 cgccccctca tcaccgagca acgctacctg cggcaacgcc gaatggacgc taaatttctc
     6481 ttcgtcgtta aaaatagctt ttaacagcga ttcagtgacg ggcgcttcca gcgaacggtg
     6541 aaatttattg ttcaggttgg caacggtgag atgcactttg ccatccagcg tatcggcaga
     6601 tggcgcgatc gttgccgcat tggctaccca cattggcgaa gcggagctga cgctggaaag
     6661 ccagtgcggt gcctggcgtg caactttttc cagtacctgc tcatcgctac cactgaatcc
     6721 caactgacgc agcaccggaa taaacggacg ctcgtgcggc gggatcacgg cctgggggaa
     6781 tcctgcatcg gcaagggttt tcattttcag taagccctgt ttcgccgcca gtcgcgggtt
     6841 agacacctga aaacggtgac gggtagaggc ttcattacca aacgacaggc cagcgtaatg
     6901 atgcgtcagc cctaccagcc cgtcgaaatt aacttcccag gcgttcatcg caccacctca
     6961 tcggaaaaat ccagcccggg gttaagcgtt gcgggcaaag ttaacgagtc cgactccagg
     7021 ctcgccatag gccatgcgca gtaatctgcg gcataccagg cgctggggcg atggttaccg
     7081 gatgcaccaa tgccgccgaa tggcgcggta ctggcagcac cagtaagcgg tttgttccag
     7141 ttaacaatcc ccgcccgcgc ctccagcaac agttgatcga atttttcccg ttcgggggaa
     7201 accagaccgc aagagagtcc gaagcgagtg ttattcgcca ttagaatcgc ttcctcgaaa
     7261 gaatcataac gccagacgcg caataacggt ccgaacacct cttcatctgg tacgccagca
     7321 acgcctgtca tttcaatgat ccccggcgtc agcaacgatg tctctaattg taataagcgc
     7381 ggcgcaagca gggttcgtcc gcccatcgct tccagttgct gccaggcagt aaccacctgc
     7441 tgtgcggcct gttcagaaat cagcccgcca ataaacggct gcggttcgtc atcccagttg
     7501 cccggcgtta atcgctggct gacggcaacc agacgagcaa gaaacgcatc gccctgcgct
     7561 ccgcttttga gcaataaacg gcgggcgcag gtgcagcgtt gcccggctgt gacaaacgcc
     7621 gactgaatgg tcagatggac agccgcgtcg atatccgcca cctcatcgat aattagcgga
     7681 ttattaccgc ccatctcaag ggcgagaatt ttctccggct gaccggagag ctggcgatgc
     7741 agctggtagc cggtattggc gcgaccggta aacagcaaac cgtcgagatc ctccagcgca
     7801 ctcagcgcct gacccgtttc acgcccgccc tgcaccaggt tcagcacgcc cggcggcaag
     7861 ccagcctgct gccataaacg cattaccgct tcgccactcc acggtgtcag ttcgctgggt
     7921 ttaaagataa tggtgttacc tgccagcaat gccggaacga tatgtccgtt cggcaaatga
     7981 ccagggaaat tatacggccc aaacaccgcc agcacgccgt gcgggcgatg tcgcaggctc
     8041 gccgcaccgt ccggcatttc actacgctgc tcgccggtac gaacgtgata cgccttaatt
     8101 gatatcgcga ttttattgat catcgccgtc acttcggttg ccgcttccca gcgcagctta
     8161 cccgtttctc tggcaataat cgcggttaat tcggctttat tgctttccag caatccggca
     8221 aagcgttcga caacgacctg acgttcagcc aatgagagcc gcgcccagcg cggaaacgcc
     8281 gcacgggctg cccgacaagc ctgttcgacc tgagcggcat cggcatcatt gccttgccat
     8341 aacacctcgc ccgataccgg attacgcttc acacgcgatg cgccctggcc cgttatccag
     8401 tcaccgttaa tccataaagt catgctgttt tctccactgc gcacaggcgc accagacgaa
     8461 cgcgatcccc tgcgtggcat ttgagggcat ccagttgtgc ggcggttaaa atcaaacgct
     8521 cggttgccgg atcggtacgc gccagcacca cgcggaaatg gtgataattt tcattggcga
     8581 ccaggcaggc cggaaaatcg ccctgcgcag gctgcccttc tgccacttcc accagccgac
     8641 ttttacggat ggcgcgcacg cggtcgatgt cacactcaag cgttggcccg ccgtcgaaga
     8701 tgtcgatata gttgcggtaa cgaaaacctt ctttctccag caccgcgcgg gcaggcgcgg
     8761 tttgcggatg tacctgaccg atgacgtcct gggcttcctg ggataaaaag tgggtataga
     8821 tcggatgttt cggcatcagt tctgcaataa atgccttttg cccggtgccg cagagaaaat
     8881 cggcgcggct aaaatccatc gaaaagaagc gtttaccgag gctttgccag aacggtgaat
     8941 agccgtgttc gtcaatcacc ccgcgcattt cagcaaccac tttgtcatta aacttgtcgc
     9001 gaaaagccgc cataaacata aagcgcgatt tcgacagcaa atagccgttg ccctctttgc
     9061 gccagtccgg gtcgagaaac agcgtgcaca gctcgctgct gccggtgtga tcgttactaa
     9121 gaaacagcgt cggcaatgcg ttatagacat tcagctcttt tgaggcgtga accaacgtgc
     9181 cgacccgata gttgtgccag ggatcgttca gcccaaccgc cacctcaatg gcgcaaatcc
     9241 ccgccacggt gcctgtctcg ctatcttcca gcacgaacac atagccctgc tcgcttttgg
     9301 gcagttcgcc ttgccaggtt ttgattgccc tttcgatacg cgcggaaagc gtggcttcat
     9361 tagcgggaag cgacgtcagg ccgccgcccg ttttgctggc aagctgcatc agcgccgaga
     9421 catctgagcg ctcaacggga cggatgacca tcatgatgaa cctcggctaa caaaatgttc
     9481 gcaagcagct gcaaagcgat ccagtccggt cgtcacctct tcttcgctga cattgagcgc
     9541 aggcgcaaaa cgcaccacgt tgccacccgc aatcagtacc ataacgcctg ctttcgccgc
     9601 ctcctgagag atctgtttcg cttgtccggc gtaatcggca ttcagtacac agccaatcaa
     9661 caaacctaag ccgcgaactt cactgaacaa accgtagcgg tgattaatgg tattaagacg
     9721 ctcaacaaac cagtcgtgac gctgtttaac gccattaagc atctctggtg tgttgatgag
     9781 ctccagcact ttgcccgcca ccgccgaggc cagcggattg ccgccatagg tggtgccatg
     9841 agtgccaacg gtcatcacgc tggcgcactc ttcggttgtt aacaacgcac cgaccgggaa
     9901 atcgccgccc agcgctttgg cggtagttaa cagatcaggt gtcacgccgt agtgcatata
     9961 ggcatacagt tccccggtgc gcccgacgcc ggtttgtact tcatcaaaaa tcaacagcgc
    10021 attgtggcgg tcacacaatt cacgcagacc ttgtaaaaac gcgttgctgg ctggcaccac
    10081 accgccttcc ccctggatgg gttcgacaat caccgcacag gtggcgtcgt caatcagcgc
    10141 gctggcagag ttaatatcgt tatatgcagc atgacggata tctggtggca gtggcgcaaa
    10201 atcctgtgaa taggctggct gcccacccgc actgacagta aacagcgtgc gaccatgaaa
    10261 cgcattttta aacgccatga taccgctctt atggctgccg tagcggtcgt gagcgaattt
    10321 acgcgccagt tttagcgccg cttcgttggc ttccgcaccg gagttacaaa agaagacgcg
    10381 atcggcaaac gtggcgtcga tcaatttttt cgccagtcgc agtaccggct cgttggtgta
    10441 accgttgccg gtatgccaga acttactcgc ctgttcgttc agcgcttcac gcagttccgg
    10501 atgcgcatgg cccagcgcgt tcaccgcaat gccacccgcg aagtcgatat actctttccc
    10561 ctgctgatcc cacaagcgcg aaccttcgcc acgtaccggt ataaagggtg ccggagcgta
    10621 aacaggtatc atccattcat caaagttttc acgcgtaatt ggctgagaca tagcgacctc
    10681 tacagtaaat aattcgttat ttatatgtta acaataagta atgtttgcgc tgtaaatgta
    10741 gattgcaggg ttcgtgccag ccagtgataa aagtgcataa acggcggagg ctaactggaa
    10801 atcaaggagt tataaccaaa ccatatgcat ttaaagtgca tataaagtga atacgtttgc
    10861 gatgtgggtg aataaaaaga ataaaaaacg caatgttatg cagaagtaaa atataattct
    10921 ggaattgtga tcattgacga aatttactgg aaattactgc gccattctga cgcagcgcgc
    10981 accaaaagcg ggcatttttt gcgccatcgt tgacatcatt aacaaccatc gatcaaatca
    11041 cttaacaaca ggcggtaagc aacgcgaaat tctgctacca tccacgcact ctttatctga
    11101 ataaatggca gcgactatga aatttgtctc ttttaatatc aacggcctgc gcgccagacc
    11161 tcaccagctt gaagccatcg tcgaaaagca ccaaccggat gtgattggcc tgcaggagac
    11221 aaaagttcat gacgatatgt ttccgctcga agaggtggcg aagctcggct acaacgtgtt
    11281 ttatcacggg cagaaaggcc attatggcgt ggcgctgctg accaaagaga cgccgattgc
    11341 cgtgcgtcgc ggctttcccg gtgacgacga agaggcgcag cggcggatta ttatggcgga
    11401 aatcccctca ctgctgggta atgtcaccgt gatcaacggt tacttcccgc agggtgaaag
    11461 ccgcgaccat ccgataaaat tcccggcaaa agcgcagttt tatcagaatc tgcaaaacta
    11521 cctggaaacc gaactcaaac gtgataatcc ggtactgatt atgggcgata tgaatatcag
    11581 ccctacagat ctggatatcg gcattggcga agaaaaccgt aagcgctggc tgcgtaccgg
    11641 taaatgctct ttcctgccgg aagagcgcga atggatggac aggctgatga gctgggggtt
    11701 ggtcgatacc ttccgccatg ctaatccgca aacagcagat cgtttctcat ggtttgatta
    11761 ccgctcaaaa ggttttgacg ataaccgtgg tctgcgcatc gacctgctgc tcgccagcca
    11821 accgctggca gaatgttgcg tagaaaccgg catcgactat gaaattcgca gcatggaaaa
    11881 accgtccgat cacgcccccg tctgggcgac cttccgccgc taatttagca gctctcctgg
    11941 ctcaaactgg gtcaggagaa ttaaccttga gaaaaatcaa caaactgtca gtaatgattt
    12001 gttgcctgcc gtcctttgtt ataccgtcac tgcgttttta gttgtctgac cacttctcta
    12061 ttatcaagtt tgatatagga aactccacga tgaatgctga gcgtaaattt cttttcgcct
    12121 gtcttatttt tgcgctggtc atttacgcta tccacgcttt cggtttattc gatctgctca
    12181 ccgatttacc ccacttacag acactcattc gccagagcgg attgttcggt tatagcctct
    12241 atattctgtt attcatcatt gccaccctct ttctgttacc aggaagcata ctggtgatcg
    12301 ctggaggaat agtttttggc ccgctcttag ggacactact ctcattaatt gccgccacgc
    12361 tggcctcctc gtgctcattc ctgctggcgc ggtggctggg acgtgattta ctgctgaaat
    12421 acgtgggcca tagccatacc ttccaggcca ttgaaaaagg cattgcgcgt aacggtattg
    12481 attttcttat tctgacccgc ttaatcccgt tgtttcctta caatattcaa aattacgctt
    12541 acggattaac caccatcgcc ttctggcctt atacccttat ttcggcactc acgaccctgc
    12601 ccggtattgt tatttatacc gtgatggcaa gcgatctcgc caatgaaggc attacgctgc
//
LOCUS       DAZPEB010000121        12638 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZPEB010000121 DAZPEB010000000
VERSION     DAZPEB010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12638
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.122"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..881)
                     /gene="rarD"
                     /locus_tag="ACHURJ_002854"
     CDS             complement(<1..881)
                     /gene="rarD"
                     /locus_tag="ACHURJ_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFR1281731.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMLLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRR"
     gene            complement(933..1400)
                     /gene="yigI"
                     /locus_tag="ACHURJ_002855"
     CDS             complement(933..1400)
                     /gene="yigI"
                     /locus_tag="ACHURJ_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFR1281732.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1565..2434
                     /gene="pldA"
                     /locus_tag="ACHURJ_002856"
     CDS             1565..2434
                     /gene="pldA"
                     /locus_tag="ACHURJ_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFR1281733.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2567..4396
                     /gene="recQ"
                     /locus_tag="ACHURJ_002857"
     CDS             2567..4396
                     /gene="recQ"
                     /locus_tag="ACHURJ_002857"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFR1281734.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMCKLERFGKPFMALIRAHVDGDDEE"
     gene            4460..5080
                     /gene="rhtC"
                     /locus_tag="ACHURJ_002858"
     CDS             4460..5080
                     /gene="rhtC"
                     /locus_tag="ACHURJ_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFR1281735.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQIELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGLAGALFAGFGIHLIISR"
     gene            complement(5142..5762)
                     /gene="rhtB"
                     /locus_tag="ACHURJ_002859"
     CDS             complement(5142..5762)
                     /gene="rhtB"
                     /locus_tag="ACHURJ_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFR1281736.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            5873..6895
                     /gene="pldB"
                     /locus_tag="ACHURJ_002860"
     CDS             5873..6895
                     /gene="pldB"
                     /locus_tag="ACHURJ_002860"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFR1281737.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            6903..7703
                     /gene="yigL"
                     /locus_tag="ACHURJ_002861"
     CDS             6903..7703
                     /gene="yigL"
                     /locus_tag="ACHURJ_002861"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFR1281738.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            7779..8678
                     /gene="bioP"
                     /locus_tag="ACHURJ_002862"
     CDS             7779..8678
                     /gene="bioP"
                     /locus_tag="ACHURJ_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFR1281739.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMNKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTLITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8566..9519)
                     /gene="metR"
                     /locus_tag="ACHURJ_002863"
     CDS             complement(8566..9519)
                     /gene="metR"
                     /locus_tag="ACHURJ_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFR1281740.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            9755..12016
                     /gene="metE"
                     /locus_tag="ACHURJ_002864"
     CDS             9755..12016
                     /gene="metE"
                     /locus_tag="ACHURJ_002864"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFR1281741.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPARHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTWLWLGKVKGEQFDRLSLLNDILPVYQQVLAELEKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDGVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12138..12260)
                     /locus_tag="ACHURJ_002865"
     CDS             complement(12138..12260)
                     /locus_tag="ACHURJ_002865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1281742.1"
                     /translation="MRKNDSVISVSERAQIAKWLPVMEAIRLLAKCALFMPDAA"
     gene            complement(12301..12546)
                     /locus_tag="ACHURJ_002866"
     CDS             complement(12301..12546)
                     /locus_tag="ACHURJ_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFR1281743.1"
                     /translation="MITETEINVLKVMAEKDINWNWMNLDRTLSIRQIPGFGNVVNIV
                     NKLANEGLVIIEDSGHKSMPHYHVTEKGYNFVKSENK"
BASE COUNT         2986 a         3198 c         3389 g         3065 t
ORIGIN      
        1 tttctacgct gcgtataaat cgcatccatc acaaaaattg ccagcgccac ccaaataaag
       61 gcgaaggtca ccatcttatc ggcacccggt ttttcaccat aaaacgtcac tgccaacagg
      121 aacatcagcg tcgggccaat atactggaag aagcctaacg tggagagacg caggcgtgta
      181 gcagctgcgg taaaacacaa cagcggtacg gtggtgacaa taccggcggc gatcagcagc
      241 aaattcagca acatcgggtt ttgccccata tggctggttg agctgtcggc aatagcaaac
      301 agataaatcg ccgccacggg cagcagccac atggtttcga ttaacatgcc ggtttgcgct
      361 tcaacggcaa ttttcttgcg taccagtccg tagaaggcaa aactaaacgc cagccccagc
      421 gcgatgattg gcagcgaacc aaaagtccac aactgaacca acacgccaca tatcgccaga
      481 atcaccgcca gccattgcat ccggcggaat cgctcgccga ggaaaatcat ccccagcaca
      541 atgttcacca gcgggttaat aaagtaacca aggctcgctt ccagcatatg gtgattgttc
      601 accgcccaga taaacagtag ccagttgcca ccaatcagca cggcagagac tgccagcata
      661 aaaattttct gtggcgtctg aatcagcgtt tttaaatagg accactggcg gcaaatgctc
      721 atcagcacca ccataaagaa aaacgaccag atcacgcgat gcgtcaggat ctcatcggcg
      781 ggcacgtagt aaatcagctt gaagtatgct ggcgcaatac cccaaataaa ataagcggca
      841 agagcgagta atacgccctg ccgcgtttgt tttgcatcca tcgggaatac tcatttttaa
      901 ttggtaacag cagtttacct gcttttatgt cttcaaccta ccatataggt ggcggtggca
      961 ctggcaatat aaagctgttc ttcattgtgt aattcaacgc gggcgacggc gactttattg
     1021 cctgcacgca acaggctact ggtagcagta aaacgctcgc ccctgcctgg gcgcagataa
     1081 tcaacgcgaa gatcaatggt ccccatccgc gatagccgct ggcgtagttc atcttcactg
     1141 atggtttcgt ggcgggttaa ggtacttccc acgcacacca gaccggcggc gacatccagc
     1201 gccgacgcaa tgactccgcc gtgcaaaatg ctttgcgccc agttacccac catcatcggc
     1261 tgatttttaa aggccagctg tgcgaactct ttttcgtaac gctccagctc catccctaac
     1321 gcgcggttaa atggcatgtg ataaacaaac atctcaccca ctaatttcag ggcttgttca
     1381 gcggtcagta cggcagacat atcatcctta cacttcattg gttaatgaaa tgttgatttt
     1441 atgcttcttt gttgttggtt tctactttag gaagggataa ctaacggcaa tggagttaag
     1501 tatgtaaaat agcccgcaga aaaacattca ccttatcaat aattcgttac ggagaacacg
     1561 accgatgcgg actctgcagg gctggttgtt gccggtgttt atgttgccta tggcagtata
     1621 tgcacaagag gcaacggtga aagaggtgca tgacgcgcca gcggtgcgtg gcagtattat
     1681 cgccaatatg ctgcaggagc atgacaatcc gttcacgctc tatccttatg acaccaacta
     1741 cctcatctac acccaaacca gcgatctgaa caaagaagcg attgccagtt acgactgggc
     1801 ggaaaatgcg cgtaaggatg aagtaaagtt tcagttgagc ctggcatttc cactgtggcg
     1861 tgggatttta ggcccgaact cggtgttggg tgcgtcttat acgcaaaaat cctggtggca
     1921 actgtccaat agcgaagagt cttcaccgtt tcgtgaaacc aactacgaac cgcaattgtt
     1981 cctcggtttt gccaccgatt accgttttgc aggttggacg ctgcgcgatg tggagatggg
     2041 gtataaccac gactctaacg ggcgttccga cccgacctcc cgcagctgga accgccttta
     2101 tactcgcctg atggcagaaa acggtaactg gctggtagaa gtgaagccgt ggtatgtggt
     2161 gggtaatact gacgataacc cggatatcac caaatatatg ggttactacc agcttaaaat
     2221 cggctatcac ctcggtgatg cggtgttgag tgcgaaagga cagtacaact ggaataccgg
     2281 ttacggcggc gcggagttag gcttaagtta cccgatcacc aaacatgtgc gcctttatac
     2341 tcaggtttac agcggctatg gcgaatcgct catcgactat aacttcaacc agacccgtgt
     2401 cggtgtagga gttatgctaa acgacttgtt ttgatgaacg gttgagtggt tggcaaatct
     2461 ggaacccagc atccaggatt accctctcag agactaaaag cattgcagtt tctcgcgcag
     2521 gcgctgaaaa tagcgcctgt ttttatttca ggcaatcggg gtgaatgtgg cgcaggcgga
     2581 agtgttgaat ctggagtccg gagctaaaca ggttttacaa gaaacctttg gctaccaaca
     2641 gtttcgcccc ggccaggaag aaattatcga cactgtgctt tccggtcgcg attgcctggt
     2701 cgtcatgccc accggtggcg gaaaatccct ttgctatcaa attcctgcct tattgctaaa
     2761 cggccttacc gtggttgttt caccgctgat ttcgttgatg aaagatcagg tggatcaact
     2821 gcaagccaac ggcgtggcgg cggcgtgcct taactcgacg caaacccgcg agcagcaact
     2881 tgaagtgatg acaggctgcc gcaccgggca aattcgtctg ctttatatcg ccccggaacg
     2941 cctgatgctg gataactttc ttgagcatct ggcgcactgg aatccggtgt tattagccgt
     3001 tgatgaagcg cactgtatct cccaatgggg ccacgatttc cgcccggaat atgccgcgct
     3061 tggtcagtta cgccagcgtt tcccgaccct gccgtttatg gcgctgactg ccacagccga
     3121 cgacaccacg cgccaggata tcgtgcgctt gctggggctg aacgatccgc tgattcaaat
     3181 cagcagtttt gaccgtccga atattcgcta catgctgatg gagaagttca aaccgctcga
     3241 tcagttgatg cgctacgtgc aggaacagcg cggtaagtca ggcattatct actgcaacag
     3301 ccgcgcgaaa gtagaagaca ccgctgcgcg cctgcaaagc aagggaatta gcgcggcggc
     3361 ctatcatgct gggctggaaa ataatgttcg cgccgacgtg caggaaaaat ttcagcgcga
     3421 tgacctgcaa attgtggtgg cgacggtggc gttcggcatg ggcatcaata aaccaaacgt
     3481 tcgcttcgtg gtccactttg atattccgcg caatatcgaa tcctattatc aggaaaccgg
     3541 tcgcgccggg cgtgatggcc tgcccgcgga agcgatgctg ttttacgatc cggccgatat
     3601 ggcgtggctg cgccgttgcc tggaagagaa gccgcagggg cagttacagg atatcgaacg
     3661 tcataaactc aatgcgatgg gcgcttttgc cgaagcgcaa acttgccgtc gtctggtatt
     3721 gctgaactat tttggcgaag ggcggcaaga gccgtgcggg aactgcgata tctgcctcga
     3781 tccgccgaaa cagtacgacg gttcaaccga tgctcagatt gccctttcca ccattggtcg
     3841 tgtgaatcag cggtttggga tgggttatgt ggtggaagtg attcgtggtg ctaataacca
     3901 gcgtatccgc gactatggtc atgacaaact gaaagtctat ggcatgggcc gtgataaaag
     3961 ccatgaacat tgggtgagcg tgatccgcca gctgattcac ctcggcctgg tgacgcaaaa
     4021 tattgcccag cattctgccc tacaactgac agaggccgcg cgcccggtgc tgcgcggcga
     4081 atcctctttg caacttgccg tgccgcgtat cgtggcgctc aaaccgaaag cgatgcagaa
     4141 atcgttcggc ggcaactatg atcgcaaact gttcgccaaa ttacgcaaac tgcgtaaatc
     4201 gattgccgat gaaagtaatg tcccgccgta cgtggtgttt aacgacgcaa ccttgattga
     4261 gatggctgaa cagatgccga tcaccgccag cgaaatgctc agcgttaacg gcgttgggat
     4321 gtgcaagctg gaacgctttg gtaaaccgtt tatggcgctg attcgtgcgc atgtcgatgg
     4381 cgacgacgaa gagtagtcag cagcataaaa aagtgccagg atgaagactc cgtaaacgtt
     4441 tcccccgcga gtcaaatgta tgttgatgtt atttctcacc gtcgccatgg tgcacattgt
     4501 ggcgcttatg agccccggtc ccgatttctt ttttgtctct cagaccgctg tcagtcgttc
     4561 ccgtaaagaa gcgatgatgg gcgtgctggg tattacctgc ggcgttatgg tttgggcagg
     4621 gattgcgttg cttggcctgc atttgattat cgaaaaaatg gcctggctgc atacgctgat
     4681 tatggttggc ggtggcctgt atctctgctg gatgggttac cagatgctac gcggtgcact
     4741 gaaaaaagag gctgtatctg cgcctgcacc acagattgag ctggcgaaaa gcgggcgcag
     4801 tttcctgaaa ggtttactga ccaatctcgc taatccgaaa gcgattatct actttggctc
     4861 ggtgttctca ttgtttgtcg gtgataacgt tggcactacc gcgcgctggg gcatttttgc
     4921 gctgatcatt gtcgaaacac tggcgtggtt taccgtcgtt gccagcctgt ttgccctgcc
     4981 gcaaatgcgc cgtggttatc aacgtctggc gaagtggatt gatggtttgg ccggggcgtt
     5041 atttgccgga tttggcattc atttgattat ttcgcggtga tgccagacgc gtcttcagag
     5101 taagtcggat aaggcgttta cgccgcatcc gacattattt ttcacgcatg cctcgccgat
     5161 gctaacagcg ctcccaccag cataaacaac gagccgaaaa tcttattcag cgccttcatc
     5221 tgctttggtc ctttaatcca tagagcaatc cgttgagcaa gggtggcgta accgatcatc
     5281 acaataatat cgactacaat agtggtgacg ccgagcacga tatactgcat cagttgcggc
     5341 tgctgtggca tgatgaattg cggaaatagc gccgccagaa acacaatact tttgggattg
     5401 gtgagattca caaaaactgc gcgctggaac aaatgtcgac gcgattgagt agaggccagc
     5461 gatttaaggt caattgcacc agcggcgcgc cactgctgga ttcccagcca aatcaagtaa
     5521 gccgcgcctg cccacttcaa cacttcaaac gcaatcactg agcgggaaaa tagcgtcccc
     5581 aaacccacgc caaccagcac aatatgaatc gccagtccgg tctgaagccc agcaatagac
     5641 gccaccgcgc cgcgataacc gtggttgagc gaggtggtca tagtgttgat tgcaccagag
     5701 cctggcgaca ggcttaaaat gatcgatgtc agcaggtagg caaaccacca ttctaaggtc
     5761 atgatgaact cccggtgtgt ctatttttgt gccacaatac gctactgtcg cagcgttgtg
     5821 tcaggcacgc taaaaaaaac gattttacgt ggtttaagag gcagattacc cgatgtttca
     5881 gcagcaaaaa gactgggaaa caagagaaaa cgcgtttgct gcttttacca tgggaccgct
     5941 gactgatttc tggcgtcagc gtgatgaagc agagtttact ggtgtggatg acattccggt
     6001 gcgctttgtc cgttttcgcg cacagcacca tgaccgggtg gtagtcatct gcccggggcg
     6061 tattgagagc tacgtaaaat atgcggaact ggcctatgac ctgttccatt tggggtttga
     6121 tgtcttaatc atcgaccatc gcgggcaggg acgttccggt cgcctgttag ccgatccgca
     6181 tctcgggcat gttaatcgct ttaatgatta tgttgatgat ctggcggcat tctggcagca
     6241 ggaggtccag cccggtccgt ggcgtaaacg ctatatactg gcacattcga tgggcggtgc
     6301 gatctccaca ttatttctgc aacgccatcc aggtgtatgt gacgccattg cgctaactgc
     6361 gccaatgttt gggatcgtga ttcgtatgcc gtcatttatg gcacggcaga tcctcaactg
     6421 ggccgaagcg catccacgtt tccgtgatgg ctatgcaata ggcaccgggc gctggcgcgc
     6481 gttgccgttt gctatcaacg tactgaccca cagcagacag cgatatcgac gtaacttacg
     6541 cttctatgct gatgacccaa cgattcgcgt cggtgggccg acctaccatt gggtacgcga
     6601 aagtattctg gctggcgaac aggtgttagc cggtgcgggt gatgacgcca cgccaacgct
     6661 tctcttgcag gctgaagagg aacgcgtggt ggataaccgc atgcatgacc gtttttgtga
     6721 actccgcacc gccgcgggcc atcctgtcga aggaggacgg ccgttggtaa ttaaaggtgc
     6781 ttaccatgag atcctttttg aaaaggacgc aatgcgctca gtcgcgctcc acgccatcgt
     6841 tgattttttc aacaggcata actcacccag cggaaaccgc tctacagagg tttaaatttc
     6901 ttatgtacca ggttgttgcg tctgatttag atggcacgtt actttctccc gaccatacgt
     6961 tatcccctta cgccaaagaa actctgaagc tgctcaccgc gcgcggcatc aactttgtgt
     7021 ttgcgaccgg tcgtcaccac gttgatgtgg ggcaaattcg cgataatctg gagattaagt
     7081 cttacatgat tacctccaat ggtgcgcgcg ttcacgatct ggatggtaat ctgatttttg
     7141 ctcataacct ggatcgcgac attgccagcg atctgtttgg cgtagtcaac gacaatccgg
     7201 acatcattac taacgtttat cgcgacgacg aatggtttat gaatcgccat cgcccggaag
     7261 agatgcgctt ttttaaagaa gcggtgttca aatatgcgct gtatgagcct ggattactgg
     7321 agccggaagg cgtcagcaaa gtgttcttca cctgcgattc ccatgaacaa ctgctgccgc
     7381 tggagcaggc gattaacgct cgttggggcg atcgcgtcaa cgtcagtttc tctaccttaa
     7441 cctgtctgga agtgatggcg ggcggcgttt caaaaggcca tgcgctggaa gcggtggcga
     7501 agaaactggg ctacagcctg aaggattgta ttgcgtttgg tgacgggatg aacgacgccg
     7561 aaatgctgtc gatggcgggg aaaggctgca ttatgggcag tgcgcaccag cgtctgaaag
     7621 accttcatcc cgagctggaa gtgattggta ctaatgccga cgacgcggtg ccgcattatc
     7681 tgcgtaaact ctatttatcg taatcgttct ttatttggtc agttgtcaac ctgatacttc
     7741 gctacaatgg atactcgtta atcaaagagt tttccattgt ggcgctactt atcatcacca
     7801 cgattctgtg ggccttctcc tttagctttt atggcgagta ccttgcgggg cacgtcgata
     7861 gctattttgc ggtgctggtg cgcgttggcc tggcggcact cgtttttctg ccgtttctgc
     7921 gtacccgtgg caatagcctg aaaacagtcg gcctgtatat gctggtgggc gcgatgcagc
     7981 tcggcgtgat gtatatgctg agtttccgcg cttatctcta tctgacggtt tccgaactgc
     8041 tgctgttcac cgtactgacg ccgctctaca tcacgctgat ttatgacatc atgaataagc
     8101 gccgtctgcg ctggggatat gcttttagtg ccttgttggc ggtgattggt gccgggatta
     8161 ttcgctatga tcaggtcacc gaccatttct ggactggctt gttgctggtg caactctcca
     8221 atatcacttt tgccatcggc atggtgggtt acaaacgcct gatggaaact cgcccgatgc
     8281 cgcagcataa cgcctttgcg tggttctatc ttggcgcgtt cctggtggcg gtaattgcat
     8341 ggttcttgct gggaaatgcg cagaaaatgc cgcaaaccac gttgcaatgg ggcattctgg
     8401 tgtttcttgg cgtggtggct tccgggattg gctactttat gtggaactac ggcgcgacgc
     8461 aggtggacgc cggaacgctg ggaattatga ataatatgca cgttccggca gggctgctgg
     8521 taaacctggc tatctggcac caacagccgc actggccaac gttgattaca ggcgcgctgg
     8581 tgatcctggc ctcactgtgg gtgcatcgta agtgggtcgc tccgcgctct tcacaaacgg
     8641 cagatgatcg caggcgtgat tgcgcgctga gcgaataaac gcttccgtaa ctggctgacg
     8701 ctgctcgcca tcgcgcacgg cggcgtacag tcggctccac aagccttcgc ccagggtttt
     8761 ggtcaccacc agaccctggc gctcaaaact ctctactacc caatgcggta gcgcggcaat
     8821 acccatccgc gcggcaacca tctgaatcaa caataaggtg ttatcgacgc ttttcagtga
     8881 cggactgacg cctgccggct gaagaaaatg ccgccagaca tccagtcgac tacgctgcac
     8941 cggataaatt aatagcgtct cgctggcgag atcttccggt gtaatacgcg tttttgccgc
     9001 cagtggatgg tcaggtgcta acaccagacg cacttcatag tcgaacatcg gcgaataatg
     9061 cagaccactg cgcggcagaa tatcggacgt cattaccaga tccagctctc cctgttgcaa
     9121 ggcgggctgc gggtcaaatg tcacgcccga tttaaagtcc atctcgacct gcggccagtt
     9181 cttatggaaa ttttctaacg cgggcgtcag ccactgtata cagctatggc actcaatggc
     9241 aatgcgcaga cgcgtctgct gcggttcatt gcaggcttgc agtgcctggc taatttgtgg
     9301 cagcacctgg tttgccagtt gcaacaggat ttctccctgc ggtgtaaagc gaagcggctg
     9361 gcttttacgc acaaatagcc ggaagccaag gcgttgttcc agatcgctaa actggtgaga
     9421 cagggcggat tgcgtttgat gcaacgtcgc cgcagcggct gcgagtgagc cgcagttccg
     9481 caacgcttgt agcgttttca gatgttttac ttcgatcatg aaagtccttc acttcggcat
     9541 gaataatttg cgcttgagga atatacagta accgccaatt atggatgtgt aaacatctgg
     9601 acggctaaaa tccttcgtct tttaaattta tggtgcgttg gctgcgtttc tccaccccgg
     9661 tcacttactt cagtaagctc ccggggatga ataaacttgc cgccttccct aaattcaaaa
     9721 tccataggat ttacatataa ttagaggaag aaaaatgaca atattgaatc acaccctcgg
     9781 tttccctcgc gttggcctgc gtcgcgagct gaaaaaagcg caagagagtt attgggcagg
     9841 gaactccacg cgtgaagaac tgctggcggt agggcgtgaa ttgcgtgctc gtcactggga
     9901 tcaacaaaag caagcgggta tcgacctgct gccggtgggc gattttgcct ggtacgatca
     9961 tgtactgacc accagtctgc tgctgggtaa cgttccggcg cgtcatcaga acaaagatgg
    10021 ttcggtagat attgacaccc tgttccgtat tggtcgtgga cgtgcgccga ctggcgaacc
    10081 tgcggcggca gcggaaatga ccaaatggtt taacaccaac tatcactaca tggtgccgga
    10141 attcgttaaa ggccaacagt tcaaactgac ctggacgcag ctgctggagg aagtggacga
    10201 ggcgctggcg ctgggccaca acgttaagcc tgtgctgctg gggccggtta cctggctgtg
    10261 gctggggaaa gtgaaaggtg aacagtttga ccgcctgagc ctgctgaacg acattctgcc
    10321 ggtttatcag caagtgctgg cagaactgga gaaacgcggc atcgagtggg tacagattga
    10381 tgaacccgcg ctggtactgg aactaccaca ggcgtggctg gacgcataca aacccgctta
    10441 cgacgcgctc cagggacagg tgaaactgct gctgactacc tattttgaag gcgttacgcc
    10501 aaatctcgac acgattactg cgctgcctgt tcagggactg catgtggacc ttgtacatgg
    10561 taaagatggc gttgctgaac tgcacaagcg cctgccttct gactggctgc tgtctgcggg
    10621 actgatcaat ggtcgtaacg tctggcgcgc cgatcttact gagaaatatg cgcaaattaa
    10681 ggacattgtc ggcaaacgtg atttgtgggt ggcatcttcc tgctcgttgc tgcacagccc
    10741 catcgacctg agcgtggaaa cgcgtcttga tgcagaagtg aaaagctggt ttgccttcgc
    10801 cttacaaaaa tgccatgaac tggcactgct gcgcgatgcg ctgaacagtg gtgacacggc
    10861 agctctggca gagtggagcg ccccaattca ggcgcgtcgt cactctactc gtgtacataa
    10921 tccggcggta gaaaagcgtc tggcggcgat caccgcccag gacagccagc gtgcgaatgt
    10981 ctatgaagtg cgtgctgaag cccagcgtgc gcgttttaaa ctgccagcgt ggccgaccac
    11041 cacgattggt tccttcccgc aaaccacgga aattcgtacc ctgcgtctgg atttcaaaaa
    11101 gggcaatctc gacgccaaca actaccgcac gggcattgcg gaacatatca agcaggccat
    11161 tgttgagcag gaacgtttgg gactggatgt gctggtacat ggcgaggccg agcgtaatga
    11221 catggtggaa tactttggcg agcacctcga cggatttgtc tttacgcaaa acggttgggt
    11281 acagagctac ggttcccgct gcgtgaagcc accgattgtt attggtgacg ttagtcgccc
    11341 ggcaccgatt accgtggagt gggcgaagta tgcgcaatct ctgactgata aaccggtgaa
    11401 agggatgctg actggcccgg tgaccattct ctgctggtcg ttcccgcgtg aagatgtcag
    11461 ccgtgaaacc atcgccaaac aaattgcgct ggcgctgcgt gatgaagtgg cggacctgga
    11521 agccgcagga attggcatca tccagattga tgaaccggcg ctgcgcgaag gtttaccgct
    11581 gcgtcgtagc gactgggatg catatctcca gtggggcgtg gaggccttcc gtatcaacgc
    11641 cgccgtggcg aaagatgaca cacaaatcca cactcacatg tgttattgcg agttcaacga
    11701 catcatggat tccattgcgg cactggatgc agacgtcatc accatcgaaa cctcgcgttc
    11761 cgacatggag ttgctggagt cgtttgaaga gtttgattat ccgaatgaaa tcggtccagg
    11821 cgtctatgac attcactcgc caaacgtacc gagcgtagaa tggattgaag ccttgctgaa
    11881 gaaagcggca aaacgcattc cggcagagcg cctgtgggtc aacccggact gtggcctgaa
    11941 aacgcgcggc tggccagaaa cccgcgcggc actggcgaac atggtgcagg cggcgcagaa
    12001 tttgcgtcgg ggatgatgaa tattaacgag tcactgtaaa ttgcagtacg atgaaagagg
    12061 caaaactgcc tgataccagg cctacataat ttatgcaatt tattgaattt ttgagttttg
    12121 ttggccggac aaggcgttca cgccgcatcc ggcatgaaca acgcgcactt tgccaataat
    12181 ctgatggctt ccatcaccgg aagccatttt gctatctgcg cccgttcaga tacagaaata
    12241 accgaatcat ttttacgcaa aatataatcc tgagcgattt gttcagtaat caacgctacg
    12301 ttatttattc tcacttttca cgaaattgta tcccttttca gtaacgtggt agtgaggcat
    12361 agacttatgt ccgctatcct caataataac caaaccctcg ttagctaatt tgttaacaat
    12421 gttaacaaca ttaccgaatc ctggaatttg ccttatggat agtgtgcgat cgagattcat
    12481 ccagttccag tttatatctt tctcagccat aactttcagt acgttaattt cagtttcagt
    12541 tatcatagtc aagtgcatcc tggtggtata agtcattaag ttctccctta agtttataat
    12601 cttctagagt agcagtatgc gtatgattcc agagtgca
//
LOCUS       DAZPEB010000122        12079 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZPEB010000122 DAZPEB010000000
VERSION     DAZPEB010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12079
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.123"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(235..549)
                     /locus_tag="ACHURJ_002867"
                     /pseudo
     CDS             complement(235..549)
                     /locus_tag="ACHURJ_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
     gene            complement(664..1062)
                     /locus_tag="ACHURJ_002868"
     CDS             complement(664..1062)
                     /locus_tag="ACHURJ_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFR1281744.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQSNQSVSGEQLESAPGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSSQANNDLLSAGMELLKGKLFR"
     gene            complement(1302..3716)
                     /gene="gyrB"
                     /locus_tag="ACHURJ_002869"
     CDS             complement(1302..3716)
                     /gene="gyrB"
                     /locus_tag="ACHURJ_002869"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFR1281745.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADLLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(3745..4818)
                     /gene="recF"
                     /locus_tag="ACHURJ_002870"
     CDS             complement(3745..4818)
                     /gene="recF"
                     /locus_tag="ACHURJ_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFR1281746.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWACFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLVEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(4818..5918)
                     /gene="dnaN"
                     /locus_tag="ACHURJ_002871"
     CDS             complement(4818..5918)
                     /gene="dnaN"
                     /locus_tag="ACHURJ_002871"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFR1281747.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(5923..7326)
                     /gene="dnaA"
                     /locus_tag="ACHURJ_002872"
     CDS             complement(5923..7326)
                     /gene="dnaA"
                     /locus_tag="ACHURJ_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFR1281748.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            7623..7703
                     /locus_tag="ACHURJ_002873"
     CDS             7623..7703
                     /locus_tag="ACHURJ_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1281749.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            7933..8073
                     /gene="rpmH"
                     /locus_tag="ACHURJ_002874"
     CDS             7933..8073
                     /gene="rpmH"
                     /locus_tag="ACHURJ_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFR1281750.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            8090..8449
                     /gene="rnpA"
                     /locus_tag="ACHURJ_002875"
     CDS             8090..8449
                     /gene="rnpA"
                     /locus_tag="ACHURJ_002875"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFR1281751.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            8413..8670
                     /gene="yidD"
                     /locus_tag="ACHURJ_002876"
     CDS             8413..8670
                     /gene="yidD"
                     /locus_tag="ACHURJ_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFR1281752.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            8673..10319
                     /gene="yidC"
                     /locus_tag="ACHURJ_002877"
     CDS             8673..10319
                     /gene="yidC"
                     /locus_tag="ACHURJ_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFR1281753.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKTQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            10425..11789
                     /gene="mnmE"
                     /locus_tag="ACHURJ_002878"
     CDS             10425..11789
                     /gene="mnmE"
                     /locus_tag="ACHURJ_002878"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFR1281754.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            12032..>12079
                     /gene="tnaC"
                     /locus_tag="ACHURJ_002879"
     CDS             12032..>12079
                     /gene="tnaC"
                     /locus_tag="ACHURJ_002879"
                     /inference="COORDINATES: protein motif:HMM:NF019665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HFR1281755.1"
                     /translation="MNILHICVTSKWFNID"
BASE COUNT         2775 a         3293 c         3138 g         2873 t
ORIGIN      
        1 gcggggctga atgtggaggg gctacgcgcg ggatatcagt ctgctgaggt atatgccggc
       61 ctgacacagg ctaagtgtgc ccgggaatcg gtgcaagtat aaaaaacagc ctgttgcggt
      121 ataagaatcg accatgcaga gagagaaaac accggagtgg agggaaaagc agaaaagctc
      181 acgtggcata aggcgcggac aggggtatcg cttagttttt caatttccta tacgtgaacc
      241 gacttcgcga gagagttttt ccaggaaacg atagtcgtca taactgagag cagtttgcgc
      301 cacggtgcta ccatcagcgg ccttctgtac cagcgcgccg ttataagtaa tgcagtagtc
      361 gcccggctgt tccatatgca gctctttcag gtagttgtga acgcctgcat aagggcgacc
      421 cgtcgttagc acgacattca cgccacgggc gcgagctgcg gcaatcgcat ttttaacggc
      481 gggtgaaatg gtgtgatcgg gcagcagaag ggtgccatcc atatcgatag caatgagttt
      541 gatagccatg agttccccag gtagattagt tcctgaccca tgctaacgcg attccgctca
      601 aaaatcagta caacacccga gggaaaaggg ggatgcaacg cgggttgcat gctccccatt
      661 tgcttagcgg aagagtttcc ctttcagcag ttccatacct gcggaaagca gatcgttatt
      721 ggcttgtgaa gacacttcac cttgcggtga gagcgcatca ataatcttcg gcaattgttc
      781 tgccagtaaa ctggaagccg tactggtatc cacgccaagt ttttgcccga gatcggacac
      841 cgcatttgtg ccgggcgccg attccagttg ctcgccgcta actgattgat tgctctgctg
      901 attactcaac caggttgaga gaatggcccc taagccgcca ctttgcagtt tttccagcag
      961 cacctgaatg ccgccctgct cctcaaccca acttaaaata gcctgatatt tccccgcatc
     1021 gcctttcaga aaggcaccga caacttcatc aaaaagcccc atgataatca cctgtaaagc
     1081 gttacgtgtt gacccaaaaa gtatagatct gcgggatgat aattgcggat tgcagaaata
     1141 aaaagggcgg agatgatctc cgcccttttt ttatagcttc ttgccggatg cggcgttaac
     1201 gtcttacctg gcctacaaaa tcattaaaat tcaatacatt gcaagatttt cgtaggcctg
     1261 ataagcgtag cgcatcaggc acgctcgcat ggttaacgcc attaaatatc aatattcgcc
     1321 gctttcaggg cgttctcttc aataaacgca cgacgcggtt ctacggcgtc gcccatcagc
     1381 gtggtgaaca acaggtcagc ggcaatcgca tccttaacgg taacgcgcag catacgacga
     1441 ctttccgggt ccatggtggt ttcccacagc tgttccgggt tcatctcgcc cagaccttta
     1501 taacgctgga tggagagacc gcgacgagac tctttcacca gccagtccag cgcctgctcg
     1561 aagctggcta ccggctgacg acgctcgcca cgttcgataa atgcatcttc ttccagcaag
     1621 ccacgcagtt tctcacccag cgtgcagata cgacgatatt cgccaccagt gataaattcg
     1681 tgatccagcg gatagtcagt atccacaccg tgggtacgca cgcgaacaat cggctcgaac
     1741 aggttttgtt cggcattggt gtggacatca aacttccact ggctgccgtg ctgttctttg
     1801 tcgttcagtt cgctgaccag cgcgttcacc cagcgggtaa cggtctgctc atcagagagg
     1861 tcggcttccg tcagcgtcgg ctgatagata agctctttca gcattgcttt cggataacga
     1921 cgctccatgc ggttgatcat tttctgcgtc gcgttgtact cagacaccag tttctctaac
     1981 gcttcgccag ccaatgccgg tgcgctggcg ttggtgtgca gcgttgcgcc gtccagcgcg
     2041 atagagatct ggtactgatc catcgcttcg tcgtctttaa tgtactgttc ctgcttgcct
     2101 ttcttcactt tgtacagcgg cggctgagcg atgtagacgt gaccgcgttc aacgatttcc
     2161 ggcatctgac gatagaagaa ggtcaacagc agcgtacgaa tgtgcgagcc gtcgacgtcc
     2221 gcatcggtca tgatgatgat gctgtgataa cgcagtttgt ccgggttgta ctcgtcacga
     2281 ccgataccac agccaagcgc ggtgataagc gtcgccactt cctgagaaga gagcatctta
     2341 tcgaagcgcg ctttctcaac gttgaggatt ttacccttca gcggcagaat cgcctggttc
     2401 ttgcggttac gcccctgctt cgcagagccg cccgcggagt ccccttccac caggtacagt
     2461 tcggaaagcg ccggatcgcg ttcctggcag tctgccagtt tgcccggcag gcccgctaag
     2521 tcgagcgcac ctttacggcg ggtcatttca cgcgcgcgac gcgccgcttc acgggcacgg
     2581 gcagcatcga taattttgcc aaccacgatt ttcgcgtcgg ttgggttttc cagcaggtat
     2641 tctgccagca gttcgttcat ctgctgttca accgccgatt tcacctcaga agaaaccagt
     2701 ttgtctttgg tctgggagga gaatttcggg tccggcactt ttacggaaac gaccgcaatc
     2761 aggccttcac gcgcatcgtc accggtggcg ctgactttgg cttttttgct gtagccttct
     2821 ttgtccatgt aggcgttcag ggtacgggtc atcgccgcac ggaagcctgc caggtgagta
     2881 ccgccgtcac gctgcggaat gttgttggta aagcagtaga tgttttcctg gaagccatcg
     2941 ttccactgca acgccacttc gacgccaata ccgtcttttt cggtggagaa gtagaagata
     3001 ttcggatgga tcggcgtttt gttcttgttc agatattcaa cgaacgcctt gatgccgcct
     3061 tcatagtgga agtggtcttc tttgccgtca cgcttgtcgc gcagacgaat ggaaacgccg
     3121 gagttgagga acgacaactc acgcagacgt ttcgccagaa tttcatattc gaactcggtc
     3181 acattggtga aggtttcgag gctgggccag aaacgcacca tggtgccggt tttttcagtc
     3241 tcgccggtaa ccgccagcgg agcctgcggt acaccgtgtt cgtagatctg acggtgaatt
     3301 ttaccctcgc gctggataac cagctccagt ttttgcgaca gggcgtttac taccgaaaca
     3361 ccaacgccgt gcagaccgcc ggacacttta taggagttat cgtcaaattt accgcctgcg
     3421 tgcagaacgg tcatgatcac ttccgccgcc gatacgccct cttccgggtg aataccggtc
     3481 ggaatgccgc gcccgtcatc ctgtacagag acagagttat cggcgtgaat ggtgacgata
     3541 atttctttac agtgacccgc gagcgcttcg tcgatagcgt tatctaccac ctcgaatacc
     3601 atgtggtgca gaccggtgcc gtcatccgtg tcgccgatat acatacccgg gcgcttacgc
     3661 accgcatcca gccctttcag gactttgata ctggaggagt cataagaatt cgacatcaac
     3721 gtttctcgct catttatact tgggttaatc cgttatttta cccttttcca cggtaaacat
     3781 cttcgaattt tcgtccgaca tgtctataac gtgttcagca ctgatcgcgc tgacaaagac
     3841 ctgtgattgc gtcgctttta agcggctggc aagcagcccg cgacgctcat catcaagctc
     3901 agaggcaaaa tcatctatca ggtagagaca ccgccgcccg ctttcacggg tgaggaactc
     3961 tccttgcgcc agacgtaagg cgcacatcaa cagcttaagc tgcccacgcg ataaggtatc
     4021 ttccaccggc gcaccgtcgg cgcgaatgcg taagtccgct ttatgcgggc catgcgcggt
     4081 gtaggttagc tggcgatcgc gttcaaaatt acgttccagc acctcagcat attctgtctc
     4141 tttctcccag ccgcgctgga aagagaaagt cagagaaaac tcagggagaa attgcttaca
     4201 agtatcggcc atatcagccg cgataccggc gctatactcc gcgcgccagg tgctgatttg
     4261 ctccaccagc gggatcagct ctttatccca cgggcgtagc tgttcgtaac gtgtcacctg
     4321 gcgcagcgcc gcattgcgct gcttgagcaa tcgcttgaga ttgctccagg cggtgaaaaa
     4381 tccgggttcg ttgtgaaagc atgcccagtc gagaaatgct cttctgtatt tggggccgcc
     4441 gttgagtaaa gtaaaccctt ctggcgttat cagctgcatc ggcatcaggt gcgccagctc
     4501 cgcgacttta tgcccgtcag taccgtcgat gcggactttg ctgtcgcctt gtttgtcttt
     4561 ggttaagcca atcgccgtct cgcgctcttc gccctgtaat cgcccatgga gaacaaatgc
     4621 ctcctgctca tggcgaatga cgcgaccaat ctgcaaactg cgaaacgccc gaccatggcc
     4681 gagcgtatag atggcttcca gcacactggt tttgccactg ccgttggcac ctaccagaaa
     4741 gttaaagccg ggagataagg cgagatccgc ggtttcgata ttgcggaaat cgcggatcaa
     4801 caagcgggtg agggacatta cagtctcatt ggcatgacaa cataagccgc gctctggctg
     4861 gccgcatctt caatctgcac gctggaaacc gaatcggtca gcatcatgcg gacgttttcg
     4921 catttcagcg cgttcagaac atccagcaca tagctgacgt tgaagccgat ttccatctcc
     4981 gcaccgctat aggtaacgtc gaggatctct tccgcttctt cctgttccgg gttgttggca
     5041 gtgattttca gctggttttc gctgacataa agacgtacgc cgcggaattt ctcgttagag
     5101 agaattgccg cacgggcaaa cgcctgcttg agcagatcgc agccagcttc cagatgtttg
     5161 tccgggttct tcggtagaac gcggcggtaa tccgggaagc gaccatccac cagtttggag
     5221 gtgaagataa agtcgccaac gtgggcacga atgttattgc tgccaatctg cacgcgcagc
     5281 ggattgtcgc cgccgtcgag catacgcatc agttcaatca cgcctttacg cggcacgatc
     5341 accgaatggc ttggcaaaga ttgaccaatt ggcattgaac agactgccag acggtgacca
     5401 tcggtcgcta cggtgcgcag ttcttcgcct tcggtttcaa acagcatacc gtttaagtaa
     5461 tagcgaacat cctgatgcgc catcgaaaac tgggtcgctt caatcagacg cttcatcgtc
     5521 gcctgcggca gggtaaattc gacttcgctc tgccagtcat cgaggttcgg gaaatccgcc
     5581 gctggcaggg tagacagcga aaaacggcta cgcccggagc gcaccagcat ccgttcacct
     5641 tccagctgca cggcaatttc cgcgccttca ggcagaccac ggcagatatc aaagaatttg
     5701 cgcgccggaa cggtcgttgc tcctggctcg tgtggctgaa ccagcgcaac acgtgccacc
     5761 atttccatct cgagatcagt accggtcagc gacaacgtac cgtcagcaac ctgtaacagc
     5821 agattaccga gaatcggtag cgtaggacga ccacctaacg gaccgctcac ctgttgtagc
     5881 ggttttaata aatgctcacg ttctacggta aatttcatag gtttacgatg acaatgttct
     5941 gattaaattt gaaaaatctt ctttgatatc gtggctctct tcacgcaact gctcgatctt
     6001 acggcaggca tgaagcaccg tcgtgtggtc acgaccacca aacgcatcgc caatctccgg
     6061 cagactgtgg ttagtcagct ctttcgccag cgccatcgcc atctggcgcg gacgcgccac
     6121 cgagcgggat cgacgcttgg aaaggagatc cgcgactttg atcttgtagt actccgccac
     6181 cgtcttctga atattgtcga tggtgaccag tttttcctgc aatgccagca agtcgcgcag
     6241 cgcctcacgc acgaagtcga tggtgatcgc ccgtccggta aagttggcat tggcaatgac
     6301 gcggttcagc gccccttcca gctcacgtac gttagatcgt agacgcttgg cgataaagaa
     6361 cgccacttcg cccggcaaac gaatgtcgtt ttcgtcggcc tttttcatca ggatcgccac
     6421 acgggtttcc agctctggcg gttcgatcgc cacagtcagt ccccaaccga agcgggattt
     6481 caaacgatcc tcaacgccgt tgatctcttt cggatagcga tccgaggtga gaatgatctg
     6541 ttgattacct tccagcaggg cgttgaaggt atggaaaaac tcttcctgag atcgttcttt
     6601 attagcaaaa aactgaatat cgtcgatcag cagtgcatct acggaacggt agtagcgttt
     6661 aaactcttcg atcgcgttgt tttgcagggc tttaaccatg tcctgaacaa agcgctcgga
     6721 gtgcatataa accactttgg cattcggctt gcgcgccata atgccgttac ccaccgcatg
     6781 cagcaggtga gttttaccca gacccgtgcc gccataaagg aacaacgggt tataggcacc
     6841 gccagggtta tccgccacct ggcgagccgc cgcgcgcgcc agttggttag atttaccttc
     6901 aacgaagtta tcaaacgtgt gtttgacgtt tacgttagaa cgataggtcg gttctgccgg
     6961 ggccgggacg ttatcccaac ctgagcgcgt agaaggcgca gcacgttgcg gctgcgtttg
     7021 cgccacctgt gcaggggccg cgacgttgct cgtcactgcc gcttgtggcg tttgcgtcac
     7081 cggtttggtg ccgacttcaa aacgcagctg tggggcatcc gctccgcaga aactggttag
     7141 cagtccattg atattattaa ggtacttgtc ccgtacccaa tcgaggacaa aacggtttgg
     7201 cgcgtacagg gccagcgtgt tatcgctcag ttccgcctgc aatgggcgta tccacatact
     7261 gaattctgtg gctggtaact catcctgcaa tcgggcaaga cactgctgcc aaagcgaaag
     7321 tgacacggcg gactccactc gaacaaaagt cgataatgac taaggctgaa acattcatga
     7381 ttgttgacgt acgtcgaaaa gaccctgctt gagggtgacg cacgaaccgc tgtctgcggt
     7441 tatatgcccg atcaagatcc tgcaaaacga tcgggaccgc ggatcatagc ctaaactgcg
     7501 caagagatct tctgtttctc acagattttc ccgatttatc cacaggactt tccagaactc
     7561 gctaagtgta aacgatcctg ccgcgaggcg ggcacgattt acgccgcata ttgaaaaatt
     7621 taatgaccat agacaaaaat tggcttaatc gatctaataa agatccagga cgatccttgc
     7681 gctttaccca tcagcccgta taatcctcca cccggcgcgc catgctggtt tccactggtg
     7741 tgaggtcgta cattttccct gcgaaaaggt gcggaaaagc gcggtaaata aggaaagaga
     7801 attgactccg gagtgtacaa ttattacaat ccggcctctt taatcaccca tggcttcggt
     7861 gtccatcgtt tcatttttcg gcggatatcc aataaagcca ttgaatttat tcaagtttag
     7921 gtagaaatcg ccatgaaacg cacttttcaa ccgtctgtac tgaagcgcaa ccgttctcac
     7981 ggcttccgtg ctcgtatggc tactaaaaat ggtcgtcagg ttctggcacg tcgtcgtgct
     8041 aaaggccgcg ctcgtctgac cgtttctaag taataaagct aacccctgag tggttaagct
     8101 cgcatttccc agggagttac gcttgttaac tcccagtcaa ttcacattcg tcttccagca
     8161 gccacaacgg gctggcacgc cgcaaattac cattctcggc cgcctgaatt cgctggggca
     8221 tccccgtatc ggtcttacag tcgccaagaa aaacgttcga cgcgcccatg aacgcaatcg
     8281 gattaaacgt ctgacgcgtg aaagcttccg tctgcgccaa catgaactcc cggctatgga
     8341 tttcgtggtg gtggcgaaaa aaggggttgc cgacctcgat aaccgtgctc tctcggaagc
     8401 gttggaaaaa ttatggcgcc gccactgtcg cctggctcgc gggtcctgat agccctcatt
     8461 cgggtctatc aacgcctgat tagtccgcta ctcgggccgc attgtcgttt cactccaacc
     8521 tgttcaagct acggaattga ggcattgcgc aggtttggag tgataaaagg cagttggttg
     8581 acggtgaaac gcgtattaaa atgccaccct ttacaccctg gtggtgacga tcccgtcccg
     8641 cccggaccat ttgataccag agaacactaa cgatggattc gcaacgcaat cttttagtca
     8701 tcgctttgct gttcgtgtct ttcatgatct ggcaagcctg ggagcaggat aaaaacccgc
     8761 aacctcaggc ccaacagacc acgcagacaa cgaccaccgc agcgggtagc gccgccgacc
     8821 agggcgtacc ggccagtggc caggggaaac tgatctcggt taagaccgac gtgcttgatc
     8881 tgaccatcaa cacccgtggt ggtgatgttg agcaagctct gctgcctgct tacccgaaag
     8941 agctgaactc tacccagccg ttccagctgc tggaaacttc accgcagttt atttatcagg
     9001 cacagagcgg tctgaccggt cgtgatggcc cggataaccc ggctaacggc ccgcgtccgc
     9061 tgtataacgt tgaaaaagac gcttatgtgc tggctgaagg tcaaaacgaa ctgcaggtgc
     9121 cgatgacgta taccgacgcg gcaggcaaca cgtttaccaa aacgtttgtc ctgaaacgtg
     9181 gtgattacgc tgtcaacgtc aactacaacg tgcagaacgc tggcgagaaa ccgctggaaa
     9241 tctccacctt tggtcagttg aagcaatcca tcactctgcc accgcatctc gataccggaa
     9301 gcagcaactt cgcactgcac accttccgcg gcgcggcgta ctccacgcct gacgagaagt
     9361 acgagaaata caagttcgat accattgccg ataacgaaaa cctgaacatc tcttcgaaag
     9421 gtggttgggt ggcaatgctg caacagtatt tcgcgacggc gtggatcccg cataacgacg
     9481 gtaccaacaa cttctatacc gctaatctgg gtaacggcat cgccgctatc ggctataaat
     9541 ctcagccggt actggttcag cctggtcaga ctggcgcgat gaacagcacc ctgtgggttg
     9601 gcccggaaat ccaggacaaa atggcagctg ttgctccgca cctggatctg accgttgatt
     9661 acggttggtt gtggttcatc tctcagccgc tgttcaaact gctgaaatgg atccatagct
     9721 ttgtgggtaa ctggggcttc tccattatca tcatcacctt tatcgttcgt ggcatcatgt
     9781 acccgctgac caaaacgcag tacacctcca tggcgaagat gcgtatgctg cagccgaaga
     9841 ttcaggcaat gcgtgagcgt ctgggcgatg acaaacagcg tatcagccag gaaatgatgg
     9901 cgctgtacaa agctgagaag gttaacccgc tgggcggctg cttcccgctg ctgatccaga
     9961 tgccaatctt cctggcgttg tactacatgc tgatgggttc cgttgaactg cgtcaggcac
    10021 cgtttgcact gtggatccac gacctgtcgg cacaggaccc gtactacatc ctgccgatcc
    10081 tgatgggcgt aacgatgttc ttcattcaga agatgtcgcc gaccacagtg accgacccga
    10141 tgcagcagaa gatcatgacc tttatgccgg tcatcttcac cgtgttcttc ctgtggttcc
    10201 cgtcaggtct ggtgctgtac tatatcgtca gcaacctggt aaccattatt cagcagcagc
    10261 tgatttaccg tggtctggaa aaacgtggcc tgcatagccg cgagaagaaa aaatcctgat
    10321 tcggtgagtt ttcgctaaaa taagggcggt cagttgaccg ccttttttct tttcgtaggg
    10381 cggataagca ccgcgcatcc gccacacaaa gcaacaggaa catcatgagc gataatgaca
    10441 ctatcgtagc ccaggccacg cctccgggac gtggcggcgt tggcatcctg cgcatctccg
    10501 gcttcaaagc ccgtgaagtt gccgaaaccg tgctgggtaa actgcctaag ccgcgctacg
    10561 ccgattatct tccgtttaaa gacgccgacg gcagcgtgct cgatcagggg attgcgctat
    10621 ggttccctgg cccgaactcg ttcaccggcg aagatgtgct ggaactgcaa ggtcatggcg
    10681 gtccggtgat cctcgacctg ctgttaaaac gcattctgac cattcccggc ctgcggattg
    10741 ctcgccctgg tgagttttcc gaacgcgcgt ttcttaacga taaacttgac ttagcccagg
    10801 ccgaggcgat tgccgatctt atcgacgcca gttcggaaca ggcggcccgt tcggcactta
    10861 actcgctgca aggcgcattc tccgcacggg ttaatcatct ggtagaagcc ctcacccact
    10921 tgcgcattta cgtcgaagcg gcaattgatt tccccgatga agagatcgat ttcctctccg
    10981 acggaaaaat tgaagcccag ctcaatgacg ttattgccga tcttgatgca gtgcgtgctg
    11041 aagcacgtca gggtagtttg ttgcgcgaag ggatgaaagt ggtgattgcc ggacgtccta
    11101 acgccggtaa atcgagcctg ttaaacgcgc tggcggggcg tgaagcggca atcgtaaccg
    11161 atatcgccgg aactacgcgt gacgtgctgc gtgagcatat ccacattgac ggaatgccgc
    11221 tgcatatcat cgataccgcc gggctacgtg aagccagtga cgaagtagaa cgtattggta
    11281 tcgagcgcgc gtggcaggaa attgaacagg ccgaccgcgt gctgtttatg gtcgatggca
    11341 ccacaacaga cgccgtggat ccggcagaga tctggccgga atttattgcc cgtctgccag
    11401 cgaaactgcc gatcaccgtg gtgcgcaata aagccgatat caccggcgaa acgctgggaa
    11461 tgagtgaagt gaacggtcac gcgttaattc gtctctcggc aaggactggt gaaggcgtgg
    11521 acgtgctgcg taaccatctc aaacagagca tgggctttga caccaacatg gaaggcggct
    11581 tcctggcgcg tcgtcgccac ctacaggcgc tggaacaggc agcggaacat ctacaacagg
    11641 gcaaagcgca actgttggga gcctgggcag gtgaactgct ggcggaagag ttgcgtctgg
    11701 cacagcagaa cttaagcgaa atcaccgggg aatttacttc agacgacctg ctggggcgga
    11761 ttttctccag cttctgtatt ggtaagtaac cgcgcttacg aagccgtatt ctgactgtca
    11821 gatgcggctt cgcttcattg ttagcactcc tgttattcct caaccctttt tttaaacatt
    11881 aaaattctta cgtaatttat aatcttaaaa aaagcattta atattgctcc ccgaacgatt
    11941 gtgattcgat tcacatttaa acaattttag aatagacaaa attcttgagt attatactgt
    12001 agcctcgtgt ctttcgagga ataagtgcat tatgaatatc ttacatatat gtgtgacctc
    12061 aaaatggttc aatatcgac
//
LOCUS       DAZPEB010000123        12046 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZPEB010000123 DAZPEB010000000
VERSION     DAZPEB010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12046
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.124"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..192
                     /locus_tag="ACHURJ_002880"
     CDS             <1..192
                     /locus_tag="ACHURJ_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFR1281756.1"
                     /translation="RGAFFISALFFFNAVCIVSDNLLIIESFGEMAYNISYLTRVPGT
                     NTLLACCCLLRPEEVNSEY"
     gene            182..355
                     /locus_tag="ACHURJ_002881"
     CDS             182..355
                     /locus_tag="ACHURJ_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFR1281757.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(404..1476)
                     /locus_tag="ACHURJ_002882"
                     /pseudo
     CDS             complement(404..1476)
                     /locus_tag="ACHURJ_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
     gene            complement(1548..4292)
                     /gene="torS"
                     /locus_tag="ACHURJ_002883"
     CDS             complement(1548..4292)
                     /gene="torS"
                     /locus_tag="ACHURJ_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HFR1281758.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPMLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALY
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSTVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDVPTALMGDPRRIRQVI
                     TNLLSNALRFTDEGQIVLRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            4375..5364
                     /gene="torT"
                     /locus_tag="ACHURJ_002884"
     CDS             4375..5364
                     /gene="torT"
                     /locus_tag="ACHURJ_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFR1281759.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHYTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAGGYSQLATQQAQIDQCKQWGAEAILLGSSTTSF
                     PDLQKQVANLPVIELVNALDDPLVKSRVGVPWFQMGYQPGRYLVQWAHGKPLNVLLMP
                     GPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQEMLERHPEIDVVAG
                     TAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASDQMIWQGELAVEQA
                     IRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFYQHTSAAKK"
     gene            complement(5337..6029)
                     /gene="torR"
                     /locus_tag="ACHURJ_002885"
     CDS             complement(5337..6029)
                     /gene="torR"
                     /locus_tag="ACHURJ_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFR1281760.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGECYFLAADVC"
     gene            6149..7321
                     /gene="torC"
                     /locus_tag="ACHURJ_002886"
     CDS             6149..7321
                     /gene="torC"
                     /locus_tag="ACHURJ_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFR1281761.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEISHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDIAGKAHGDKKEEK"
     gene            7321..9885
                     /gene="torA"
                     /locus_tag="ACHURJ_002887"
                     /pseudo
     CDS             7321..9885
                     /gene="torA"
                     /locus_tag="ACHURJ_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            9882..10481
                     /gene="torD"
                     /locus_tag="ACHURJ_002888"
     CDS             9882..10481
                     /gene="torD"
                     /locus_tag="ACHURJ_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFR1281762.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLAAAVNELENCIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LWQWLPEFAARCHQYDSFGFYAALSQLLLVLVECDHQNK"
     gene            complement(10574..10879)
                     /gene="cbpM"
                     /locus_tag="ACHURJ_002889"
     CDS             complement(10574..10879)
                     /gene="cbpM"
                     /locus_tag="ACHURJ_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFR1281763.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(10879..11799)
                     /gene="cbpA"
                     /locus_tag="ACHURJ_002890"
     CDS             complement(10879..11799)
                     /gene="cbpA"
                     /locus_tag="ACHURJ_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFR1281764.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
BASE COUNT         2893 a         3092 c         3188 g         2873 t
ORIGIN      
        1 cgtggagcct tttttatatc tgccttattt ttcttcaacg ctgtatgtat agtaagcgat
       61 aacctgttga ttattgaatc tttcggggag atggcttata acatttctta cctgaccagg
      121 gtaccgggaa ccaacacctt actggcgtgt tgctgtcttt taagaccaga agaggttaac
      181 agtgaatatt gaagagttaa aaaaacaagc cgaaacggaa atcgccgact ttatcgcgca
      241 aaaaatcgcc gagctgaaca agaatacagg gaaagaagtc tctgaaattc gcttcaccgc
      301 acgagaaaaa atgaccgggc ttgaaagtta tgatgtcaaa atcaaaataa tgtgattttg
      361 tgaacatcac cccgtgcgag gtgatgttcc gcttgttgct aatttagtga tcaatcattg
      421 gcgcttgtgg aattaagcgt cggtacaatt cctccggcac cgggctttgc catactcccg
      481 catacattgc gtaaccaatc accgcgaaca taatccccag aaccagtagc gtcattaacc
      541 agccagacaa cgcaaaagct tttttatttg ccgcaggttt ttgcagtgaa aaggtcaatg
      601 ttgaggctac cggacatgac tctacgcaag tcatacagcc ggtacattcc actgttcgta
      661 cctgaattaa tttatctacc gggatccgtg atgggcaatt ttttgcgcat ttgccacagc
      721 cgatacaact ttcggcattg cgacgaatct taaacggcga taatagcgaa accacgccca
      781 tcagcgcgcc atatgggcaa agataacgac accaggcatg gcgaataaac aggctggcaa
      841 tcagcaaaac ggtcacgctg attaatgtcg cggtccccat atgacgaaag aaatcgagca
      901 tttttacgtc catcaccacg ctgtagggcg acaacataaa atagtgaatc gcctgagcgg
      961 gcatcaataa cgcgatatag agaaaaaaac tcaacagcaa atacttcacg ccgcgcagag
     1021 gaatatccag ccagcgggga aggacacatt gccgaccaaa cagtttgtta ccgagatcgc
     1081 cgattaattc agaaagcgta ccaaccgggc ataaccatga gcaaaaggcc tttttgagta
     1141 atagactgat gacgataaaa gcgaccaata acagcatcgc ggagcgtgga cggacggtaa
     1201 ctgacctgtt gtaaggctat atttcagatt catcagaccg gcaatcggta gccagccttc
     1261 gatacctccc ggtctggcga caaatgtcgt gctacttgcc gtttcgtaat agcgcaccca
     1321 ataccagaac gtgatggcaa tataaatatt cattgccaac agtaataatt gcgtcgcttt
     1381 acgccaggtc gtggcattgc gccagtcatt ccacggtaat ttgccgcccg tcgtgcctgg
     1441 ccgccgctgc cagcgggttc tttttttctc tgccatgatt ttgtcagtcc gttaagttgc
     1501 ataccaaatg ccactatttt agttgttctt aactggctga tattgattca aatcgcgttc
     1561 aggtctttct tatgcaacca tgcttccaga gcggcaacac tgcgtgtaat ttcttcgtgt
     1621 ggaagggggg cagataatgg ctgctgctcc agttgtgcgc atagctggct ggcgatacgc
     1681 attcccaaac tcgagcaact gctttttagc tgatgcgcgg cacgctttat tttctcgcta
     1741 tcctgactgg cgcgggcaat gtcgatttca tcgagaagcg gcagggcatg ttgtgtaaat
     1801 aataccagcc attcgtggat cttctccgtc cccattaact gagcatcttc attgagttgc
     1861 gatacatcca gcgattgatc gttattgact tgcagttgga gatagtgcgc cagtaactga
     1921 ccgagcactt cacgcggcac cggtttaggg ataatcccgc ggaataatga actggtacgc
     1981 tggcgcagcg tttcgtcaat gacatgggcg ctaaagccaa tcaaaaccag cgacggatat
     2041 tgctgtgcca gttgtcgggc aagcgtaatg ccgtcgacat ccggcagatc aaaatccacc
     2101 agtgcggcag caaacggttc gctattttgc agtgtctcta aagcctgcgc ggcattgcca
     2161 acagcaacaa cctgcgcacc actggtgttc agcatctcaa cggtaattcg ctgggttagc
     2221 gggttatctt caattaacag caaacgtaaa ccgtcaagac gcaccgcctg attgactgtt
     2281 tttggcacgg gtgccgtggc aacacgtaac ggcaagcgta aacaaaaaca gcttccaacc
     2341 tccggcgtgc tggtggcgct cagttcgccg cccatcgcct gggccagacg gctactgata
     2401 gtcagtccca gcccggtgcc gccgcgtttg ccgcttacct gcacaaatgg ctggaagatt
     2461 tctgccagtt tcgcgggatc aataccgcag ccgctgtctt ccacttcgac cagccattgc
     2521 tcgccatcag tgcggctacg taaaacgatc tgcccttcgt cagtaaaacg cagggcgttg
     2581 ctcaacaggt tggttataac ctgacgaata cgtcgcggat cgcccattaa cgcggtcggt
     2641 acatcgtcgg caattgctgt tgccaggcga atcgggcgac ctttcacccg tccgctcatt
     2701 aattgcaggg tactttccag cagcgggcgc ggttcaaagg gctcatcgct gaccgaaaca
     2761 ttcttgccac ctgcttcgat agccgaataa tcgagaatat cgttgaggat ggtcagcaac
     2821 gattcgccag agtcggtaat cgcccgcaaa tcatcacgct gggcgttaag tgcggggtta
     2881 tctgccagca gttgagcagt gccgagaata ccgtacagcg gtgtgcggat ctcatggctc
     2941 atcgccgcca gaaaggccga ttttgcctgg ctggcttttt ctgcttccgc ccgtgcctgt
     3001 cggtgttcta tcaccagttc ctgcaattca gctgtacgcg ctttgacctg cgccgccagc
     3061 tgttcacggt ggcgattcag tgcatgaaca gtgctgcgaa acgcatccat cagccgcccg
     3121 atggtatcca gctcccgtac gccagcggtt tccgggaaag gggagtcaat atcaccgtcc
     3181 agcagccgtt gcagcgcctg cgtttgttcg gcaagtggac gcgtgactga gcgataaacc
     3241 acgcgccaaa ggatcagaat cagtgcgcaa agtgaaacca tccccagcaa taacaggctg
     3301 tattgcccgc gtgcactggc tttttccaga tgcgccagtc cgtgctgatt acgcagctca
     3361 atggtgtcga ccagctgact gacttcgcta ctaaactgcg cgaactgggc gatgttattt
     3421 tgtgcgagag tttgtaggtg attgctgatt tcactgtcct gctgatacag cgccagcaaa
     3481 tcgctatatt ggctaacggt agttaacgtt gttgcgacct gcgcacgaac acccggatct
     3541 tcaatgcgta tttgccgacg ttgcagaatt ttcaccgcat tattgagctg cttttccagc
     3601 attggtgcat ttttctggat ctgctccagc cccagattca tcaccatttg ctgcacccgc
     3661 agagcgctaa ggcgcagttc attcatctgg ttaacatact caagatcgat atcaatcagc
     3721 cgatcgagtg cactttcagc agcctgacgc tgatcttgtt cgatcaaatc gtaaatcccg
     3781 gcctgggtcg ctccagcgga agttgtcgca ttattcgcct gaccttgcgc caggcgtgcg
     3841 atctcatcgg cggcagcgac tatctgctga ctgagttgct gttgttgctg gcgtagttgc
     3901 agacgccgcc ccaccagttc cccttgctga cgtaacgaac gggagatctc ctgctcctgt
     3961 tgttcaatag cggtggtatc aaaaccttgt tcccgtaacg cttgcagcaa cgcattaatc
     4021 ttcaggcttt gtgcggtgag cattcgcccc tgcgcctgcc acatcttttc gttatcggca
     4081 ctggtcaggt tctgcgcggc gaaaagttcc caggcgctgg cttcgctcaa ctggcgcgcc
     4141 atattcatgg taggaatcaa tgcctgagtg ttgtcttttt ccacctggct gataaagcgc
     4201 aggttgtacc atcccaccag ggtactggtc agggttaaca gcgccatcag ggcaaagccc
     4261 atccagagtc ttcgggtcag ggttaaattc acggtcggtg cactttaggt gaaaaaggtt
     4321 gagtcgcaaa gcagaatgca tctagcataa agccttatta ttgattaggc tatcatgcgc
     4381 gtactgctat ttttacttct ttcccttttc atgttatcgg cattttcggc tgataacctg
     4441 ttgcgctggc atgatgcgca gcattacacg gtgcaagcct ctacgccgct taaagccaaa
     4501 cgcgcatgga aactgtgcgc gctttatccc agcctgaaag attcatactg gttatcgttg
     4561 aactatggta tgcaggaggc aggcggctac agccagttgg ctacccagca agcacaaatc
     4621 gaccagtgta aacagtgggg cgcagaggcc attttgctcg gtagtagcac tacgtcattt
     4681 cccgacctgc aaaagcaggt agcaaatctg ccggtgatcg aactggtaaa tgctcttgat
     4741 gatcccctgg tgaaaagccg cgttggtgta ccctggtttc agatgggcta tcaacctggg
     4801 cgatatttgg tgcaatgggc gcacggtaaa ccactgaacg tgctgttgat gcccggaccc
     4861 gataatgccg ggggcagtaa agagatggtc gagggttttc gcgcagccat tgccggaagc
     4921 ccggtgcgta ttgttgatat tgcgcttggt gataacgata ttgaaatcca gcgtaacctg
     4981 ttgcaggaga tgctggaacg ccacccagaa atcgacgtcg ttgccggaac ggccattgcg
     5041 gcagaggcgg caatgggtga agggcgtaac ctgaaaacgc cgcttaccgt ggtgtcgttt
     5101 tatctttcac atcaggtgta tcgcgggctg aagcggggaa gagtgattat ggctgccagc
     5161 gatcaaatga tctggcaggg ggaactggcg gttgagcagg ccatcaggca attacagggg
     5221 caatcggtgt ctgataatgt cagcccaccg attttagttc tgacgccgaa aaatgccgac
     5281 cgtgaacata ttcgccgctc gctgtcacca gggggatttc gtccggtcta tttttatcag
     5341 cacacatcag cggctaagaa ataacattca ccatgttgcg tcaccagtaa atccgcgctg
     5401 agtttatgac gtaaacgacg aattaacaca tcgacggtgc gcaggtcagg gttttccacc
     5461 cgacgcgcag aaagcatacg tagcagacgt tcacggctga gaatttcgcc cggattcgtc
     5521 acaaatgcca ccaacatttc atactctgcg cgggtcagtt taatcggctc gccatcccgc
     5581 tccagcgtat ggcgcgacac attcaggcaa taaccggcaa agcgatagca gttgtcctga
     5641 gtgtgcggtt gagcttgtcg cgcgaggtcg attcgccaga gcagattttt cacccgtact
     5701 accagttcgc gcagttccag cggtttggtg acgtaatcgt ctgcgcccat ttccagccca
     5761 acaatacggt caatccgatc gctgcgtccg gtaaccagaa taatccccac cgttgagcgt
     5821 tctcgcaggg cgcgggttaa catcaggcca ttttcatcgg gtaagttgat atccagcaga
     5881 attaaatcta ccggctgatt ctgcataatt tcccgcagcc cggcaccgct cgctgtaacg
     5941 gaaacggtat acccctcctg agtgaagtaa gattgtaatc gcgcctgggt aaccggctca
     6001 tcttcaacaa taacaatgtg atgtggcatc agagggtttt actcattctg ttcatattct
     6061 gccgtaagcc gttcatcctg accagtgccg ctgttcatat ttgctcatta agatcgcttc
     6121 actaaaccat aattttacag gggctattat gcggaaactc tggaacgcgc tacgccgacc
     6181 cagtgctcgt tggtcggtac tggcgctggt cgcaattggg attgtgattg gcattgcgct
     6241 gattgtattg ccacacgttg ggatcaaagt caccagcaca accgaatttt gtgtcagttg
     6301 ccacagtatg caaccggtgt atgaagaata taaacagtcg gtgcatttcc agaacgcctc
     6361 cggcgtgcga gctgaatgcc atgactgtca tatcccgccg gatattccag gcatggtgaa
     6421 gcgcaaactg gaagcgagca atgatatcta ccagaccttt attgctcact ccattgatac
     6481 acctgaaaaa ttcgaagcca aacgcgcgga acttgccgag cgtgaatggg cgcgaatgaa
     6541 agaaaacaac tcggcaacct gccgctcctg ccataactac gatgcgatgg atcatgcgaa
     6601 gcagcatcct gaagcggcac gtcagatgaa ggtggcagcg aaagataatc aatcctgcat
     6661 cgactgtcat aaaggtattg cccaccagtt accggatatg agtagcggct tccgtaagca
     6721 gttcgatgag ctgcgcgcca gtgctaatga cagtggtgac acgctgtact ctattgatat
     6781 taagccaatt tatgcggcga aaggcgataa agaagcctct ggttctctgc tgcctgcttc
     6841 ggaagtgaaa gtccttaaac gtgacggcga ctggctgcaa attgaaatta ccggctggac
     6901 ggaaagcgcc ggacgtcagc gtgtactcac ccaattccca ggtaaacgca tctttgttgc
     6961 ctcgattcgt ggtgatgtgc agcagcaggt aaaaacgctg gagaaaacca ccgttgccga
     7021 caccaatacc gagtggagca agttgcaggc cactgcgtgg atgaagaaag gtgatatggt
     7081 gaacgatatc aaaccgatct gggcttatgc ggattcgttg tacaacggca cctgtaacca
     7141 gtgccacggc gcaccggaaa tctctcactt tgatgctaac ggctggattg gcacgctcaa
     7201 cggcatgatt ggctttacca gtctcgataa acgtgaagaa cgcaccttgt tgaaatatct
     7261 gcaaatgaat gcgtctgaca tcgcaggtaa ggctcacggc gataagaagg aagaaaaata
     7321 atgaacaata acgatctctt tcaggcatca cgtcggcgtt ttctggcaca actcggcggc
     7381 ttaacagtcg ccgggatgct ggggccgtca ttgttaacgc cgcgcagtgc gactgcggcg
     7441 caagcggcgg atgtcatctc gaaagagggc attcttaccg ggtcgcactg gggggctatc
     7501 cgcgcgacgg tgaaggatgg tcgctttgtg gcggcaaaac cgttcgaact ggataaatat
     7561 ccgtcgaaaa tgattgccgg attgccggat tgccggattg ccggattgcc ggatcacgta
     7621 cacaacgcgg cgcgtattcg ttatccgatg gtacgcgtgg actggctgcg taagcgccat
     7681 ctcagcgata cctcccagcg cggtgataac cgttttgtgc gcgtgagctg ggatgaagcc
     7741 ctcgacatgt tctatgaaga actggaacgc gtgcagaaaa ctcacgggcc gagtgccttg
     7801 ctgaccgcca gtggttggca atcgacgggg atgttccata acgcttcggg gatgctggcg
     7861 aaagctattg ccttgcatgg taatagcgtt ggtacgggcg gagattactc taccggtgct
     7921 gcgcaggtga tcctgccgcg cgtagtcggt tcgatggaag tgtatgaaca gcaaacctcc
     7981 tggccgctgg tattgcagaa cagcaaaacc attatgctgt ggggctccga tttgctgaaa
     8041 aaccagcaag cgaactggtg gtgcccggat cacgatgttt atgaatatta cgagcaacta
     8101 aaagcgaaag tcgccgccgg tgaaattgag gtcatcagca tcgatccggt tgtcacatcc
     8161 acccatgagt atctggggcg cgagcatgtg aagcacattg cggttaaccc gcaaactgac
     8221 gtgccgctgc aactggcgct ggcgtatacg ctgtacagtg aaaacctgta cgacaaaaac
     8281 ttcctcgcta actactgtgt aggttttgag cagttcctgc cgtatctgct gggtgagaaa
     8341 gacggtcagc cgaaagatgc cgcatgggct gaaaaactga ccggcattga tgccgaaacc
     8401 attcgtgggc tggcgcggca gatggcggcg aacagaacgc aaattattgc tggctggtgc
     8461 gtgcagcgta tgcagcacgg tgaacagtgg gcgtggatga ttgtggttct ggcggcgatg
     8521 ctggggcaaa ttggcctgcc aggtggtggt tttggttttg gctggcacta caacggcgca
     8581 ggcacgccgg ggcgtaaagg cgttattctg agtggtttct ccggctctac gtcgattccg
     8641 cctgttcacg acaacagtga ctacaaaggc tacagcagca ccattccgat tgcccgtttt
     8701 atcgatgcga tcctcgaacc ggggaaagtg atcaactgga acggtaaatc ggtaaaactg
     8761 ccgccactga aaatgtgtat ttttgccgga actaacccat tccatcgcca tcagcagatc
     8821 aaccgcatta tttaaggctg gcgcaagctg gaaacggtta tcgccataga taaccagtgg
     8881 acctcaacct gccgctttgc cgatatcgta ctgcctgcga ccacgcagtt tgagcgtaac
     8941 gatctcgacc agtacggcaa ccactccaac cgtggcatta tcgccatgaa acaggtggtg
     9001 ccgccgcagt tcgaggcgcg caacgacttt gatattttcc gcgagctgtg ccgtcgcttt
     9061 aatcgcgaag aagcctttac cgaagggctg gacgaaatgg gctggctgaa acgcatctgg
     9121 caggaaggtg tacaacaagg caaaggacgc ggcgttcatc tgccagcgtt tgatgacttc
     9181 tggaataaca aagagtacgt cgagtttgac catccgcaga tgtttgttcg ccaccaggca
     9241 ttccgcgaag atccggatct cgaaccgctg ggcacgccga gtggcctgat tgagatctac
     9301 tcgaaaacca tcgccgatat gaactacgac gattgtcagg ggcatccgat gtggtttgag
     9361 aaaatcgaac gctcccacgg tgggcccggc tcgcagaagt atccgttgca tctgcaatct
     9421 gtgcatccgg atttccgact tcactcgcag ttatgtgagt cggaaactct gcgtcagcaa
     9481 tatacggtag cgggtaaaga gccagtattc attaacccgc aggatgccag cgcgcgcggt
     9541 attcgtaacg gtgatgtggt acgcgtcttt aacgctcgcg gtcaggtgtt ggcaggggca
     9601 gtagtttctg accgctatgc acccggcgtg gcacgaattc acgaaggggc atggtacgat
     9661 ccagataaag gcggcgagcc tggtgcgctg tgcaaatacg gtaaccccaa cgtgctgacc
     9721 atcgacatcg gtacttcgca gctggcgcag gcgaccagtg cgcacactac gctggtggaa
     9781 attgagaagt acaatggagc agtggagcag gtgacggcgt ttaacggccc tgtggagata
     9841 gtggcgcagt gcgaatatgt tcccgcgtcg caggtgaaat catgaccacg ctgacagcac
     9901 aacagattgc ctgtgtttac gcctggctgg cgcagttgtt ctcccgtgaa ctggacgatg
     9961 aacaactgac gcaaatcgcc agtgcgcaga tggcggaatg gttttcgttg ctgaaaagcg
    10021 aaccgccgct ggctgcggcg gtgaatgagc tggaaaactg tattgccacg ctgacagtac
    10081 gtgacgatgc ccgtctggaa ctggccgctg acttttgcgg cctgtttttg atgactgata
    10141 agcaggcggc gctgccgtat gcatcggcct acaaacagga cgagcaagag attaaacgct
    10201 tgttagttga ggcagggatg gaaaccagcg gcaatttcaa cgaaccggca gaccatctgg
    10261 cgatctatct cgagttgttg agtcatctgc atttttcgct gggagagggg accgttcctg
    10321 cgcgaagaat cgacagtttg cggcaaaaaa cactgacggc gctgtggcaa tggttaccag
    10381 agtttgctgc gcgttgccat cagtatgaca gctttggttt ttacgcggca ctaagccagt
    10441 tattgctggt gttagtggag tgcgaccacc aaaacaaata acgtcgttta tgcgcctgaa
    10501 aagacgcgtt tagcgtcgca tcaggcatta tggcgcagtt gccggatgcg tcatcacatc
    10561 cggcaaacac acatcacgga tgagctacaa accgggaaag ccgctggcgt agcaggcggt
    10621 tttcctgctt caggtgtgca atatcatcca ttaacgtcag cgccaccgcg atccccggcc
    10681 agtccagagc cagttcatga cgcaggcgta ccgcgcgttg caccacaatg gcggcatggt
    10741 cgtcaaatac ccaggttgtt tcctggatct cacgcggttc aaccaccccc aaaccgacaa
    10801 tttcattcaa ctcctcttca gagatgccgg tatgcaggca aaattcggta atagtaaaag
    10861 tcaccgtaac attagccatc atgctttccc ccaatcttta cgtggatcaa aagacgactg
    10921 ggcgtctgcc agttgctgcc acagcgcggc agtgttttca tccggtttcg gcggcatcac
    10981 gattttcagt accgcataca gatcgccggt ctgttttttg ctcaccagac ctttgccttt
    11041 aacgcgcaat cgttgcccgg cctggctgcc tggcgggata gtcagcaaaa tgctttcttt
    11101 cagtgttgga acggtgactt tagcacccag cgccgcttcc cacgggctaa ccggcaccac
    11161 aatttccaga tcctggccga caatatcgaa cagcggatgt ggcgcaatat gaatcaccag
    11221 ccacaaatcg ccatttggac caccgttttc gcccggcgtc ccctggcctt tcagacggat
    11281 gcgttgacca ttgccgacgc ccgccgggat cttcacattc agcgttttcg ggatttcctg
    11341 ttcgatcatg ccaaaggcgt tataaaccgg caggttatag ctgatggtac gcttatgctc
    11401 agtcagcgtt tcttcgagga ataccgccac ttcgatttca atatcgtggc cgcgtgcggc
    11461 ggggcgttga cggctctggc gggcatgctg accgaaaatt gacgagaaga tatcgtcaaa
    11521 atcttcggca ttaaaactct gaccgtcgcc atgatggaac tgacggttaa attgcggatc
    11581 gttgcgatgt tgccacatct gatcatactc agcgcgacgt tgttcatcac ttaacacttc
    11641 ccaggcttca gcgacctctt tgaagcgggc ttcggcatcc ggttctttgc tgacatcagg
    11701 atggtattta cgggccagtc gacgataggc ggtcttgatt gtcttgagat cgtccgtcgg
    11761 tttcacgccc atgatggcgt aataatcctt taattccata gcgttatctc gcgtaaatca
    11821 acacaaattg aaggaacccc tgtaaggtaa ctcctataag tgtagggtaa tcctcaaaat
    11881 ttcatatgcc aacacagaat atgttattga aatcatcgtg gagaggaggt cgccatcaat
    11941 atgggttgct gaacatattt taaacaggtg aaaaagggtg agcgattttt gatagttgaa
    12001 ccaggcactt taagtttaac tagggcgtca ttatttatta aatttt
//
LOCUS       DAZPEB010000124        12043 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZPEB010000124 DAZPEB010000000
VERSION     DAZPEB010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12043
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.125"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..413)
                     /gene="yghX"
                     /locus_tag="ACHURJ_002891"
     CDS             complement(<1..413)
                     /gene="yghX"
                     /locus_tag="ACHURJ_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFR1281765.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYVLTTQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVTKAGYIALAPDGLSSVG"
     gene            569..1609
                     /gene="gpr"
                     /locus_tag="ACHURJ_002892"
     CDS             569..1609
                     /gene="gpr"
                     /locus_tag="ACHURJ_002892"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFR1281766.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGVSRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(1649..2143)
                     /locus_tag="ACHURJ_002893"
     CDS             complement(1649..2143)
                     /locus_tag="ACHURJ_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFR1281767.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            2334..3218
                     /gene="yghA"
                     /locus_tag="ACHURJ_002894"
     CDS             2334..3218
                     /gene="yghA"
                     /locus_tag="ACHURJ_002894"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFR1281768.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGQAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQQTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(3490..3915)
                     /gene="exbD"
                     /locus_tag="ACHURJ_002895"
     CDS             complement(3490..3915)
                     /gene="exbD"
                     /locus_tag="ACHURJ_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFR1281769.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(3922..4656)
                     /gene="exbB"
                     /locus_tag="ACHURJ_002896"
     CDS             complement(3922..4656)
                     /gene="exbB"
                     /locus_tag="ACHURJ_002896"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFR1281770.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLFNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            4908..6095
                     /gene="metC"
                     /locus_tag="ACHURJ_002897"
     CDS             4908..6095
                     /gene="metC"
                     /locus_tag="ACHURJ_002897"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFR1281771.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            6235..6894
                     /gene="yghB"
                     /locus_tag="ACHURJ_002898"
     CDS             6235..6894
                     /gene="yghB"
                     /locus_tag="ACHURJ_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFR1281772.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGMLFVVIKKKYCN
                     A"
     gene            complement(6934..7833)
                     /gene="yqhC"
                     /locus_tag="ACHURJ_002899"
     CDS             complement(6934..7833)
                     /gene="yqhC"
                     /locus_tag="ACHURJ_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFR1281773.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            8027..9190
                     /gene="yqhD"
                     /locus_tag="ACHURJ_002900"
     CDS             8027..9190
                     /gene="yqhD"
                     /locus_tag="ACHURJ_002900"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFR1281774.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAADNYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            9295..10122
                     /gene="dkgA"
                     /locus_tag="ACHURJ_002901"
     CDS             9295..10122
                     /gene="dkgA"
                     /locus_tag="ACHURJ_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFR1281775.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVIIAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            10322..11248
                     /locus_tag="ACHURJ_002902"
     CDS             10322..11248
                     /locus_tag="ACHURJ_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFR1281776.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            11299..11556
                     /gene="yqhH"
                     /locus_tag="ACHURJ_002903"
     CDS             11299..11556
                     /gene="yqhH"
                     /locus_tag="ACHURJ_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFR1281777.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            11636..>12043
                     /locus_tag="ACHURJ_002904"
     CDS             11636..>12043
                     /locus_tag="ACHURJ_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1281778.1"
                     /translation="MRRWHTCFFILLFCGFSGNVLCKESINEAIERLYQPLTSNDDIP
                     ITDEFSLDLRSNMSATPGKMTPWLNNNLLCRCVDDAFVSAEIIQSTHLNGGRTHVLLQ
                     LVIKQNRTDIHRLLGLMLVRENDNHRWLIEDIDD"
BASE COUNT         3118 a         3056 c         3002 g         2867 t
ORIGIN      
        1 ccaacggaac ttaagccgtc aggtgccagg gcgatatacc ccgccttcgt cactcgccgt
       61 gccacatctt cgatatacgg attcagtcca cgattctcat gcaccaccac cacggctggc
      121 gttttgccgc tcatttttgc gggtttcacc agataacccc gtacctcgcc gtgaccattt
      181 ggcgaaggat acgtgatgta ctcagcaaca atctccgggt cggtaaactc tacctgagtc
      241 gtcagcacat aatttggctt gagcaaatca aacaacgcta atgccgtcat cccgcccacc
      301 gcatacttcg ccgcaaggtt gaggaactca cgtttcgaaa ttttcccgtg agcgtaatag
      361 tcgtagtaat ccaacaactc ttgtgggaaa tctttggcgg ttaaacgcgg catcgttgca
      421 ttcctcaact ggtgtttttt aagcaaagca taagcgcgtg tttttgccca gtttttcgtc
      481 actctgtgag ccagactacg ggatacgcgc tggcgaatcg ctaaactaga aacattgttt
      541 cgaaattgaa cggtggaaag gagaggtcat ggtctggtta gcgaatcccg aacgttacgg
      601 gcagatgcag taccgctatt gcggaaaaag tggtttacgc ctgcccgcgt tatcgctcgg
      661 tttatggcac aatttcggtc acgttaacgc gctggaatca cagcgtgcaa tcctgcgtaa
      721 agcgtttgat ttaggcatta cgcactttga tttagccaac aattacgggc cgcctccagg
      781 aagcgcagaa gagaactttg gtcgcctgct gcgggaggat tttgccgctt atcgcgatga
      841 actgattatc tctaccaagg ctggctacga tatgtggccc ggcccttacg gctctggcgg
      901 ttcacgtaaa tacctgctcg ccagcctcga ccaaagcctg aagcgtatgg ggcttgagta
      961 tgtcgatatc ttttactctc atcgcgtcga tgaaaatacg ccgatggaag aaaccgcctc
     1021 tgcgctggct catgcggtac aaagcggtaa ggcgctgtat gtcgggatct cctcttactc
     1081 gccagagcgg acgcaaaaaa tggtcgagtt gctgcacgag tggaaaattc cgcttttaat
     1141 tcatcaacct tcgtacaatt tactgaaccg ctgggtggat aaaagcggcc tgctggatac
     1201 cctgcaaaat aacggcgtgg gctgtattgc ctttactcct ctggctcagg gattgctgac
     1261 cggaaaatat ctcaacggca ttccgcaaga ttcacggatg catcgtgaag ggaataaagt
     1321 tcgtggtctg acgccgaaaa tgcttaccga agccaacctc aacagcctgc gcttattgaa
     1381 tgaaatggca cagcagcgtg gacaatcaat ggcgcaaatg gcgttaagct ggttgctgaa
     1441 agatgatcgc gtgacgtcgg tattgattgg tgtcagccgc gcggagcaac tagaggagaa
     1501 cgtgcaggcg ctgaataatc tgacatttag caccgaggag ctggcgcaga ttgatcagca
     1561 tatcgccgat ggcgagctga atctgtggca ggcgtcttcc gataaatgac ctgttaataa
     1621 cgggcgcgcg aaccgcgccc gaatcagatc agtgattatg acgagtcagt cggtcaagat
     1681 agcccatcac aaatgcagag agcacaaacg tcagatggat aatgacgtac cacatcagtt
     1741 tgttatccgg gacatttttc gcatccataa agacgcgcag taagtgaatg gaagaaattg
     1801 ccacaatcga cgctgctact ttgtttttca gcgacgttgc gtccattttc cccagccagt
     1861 tcagcttctc tttgttctcg gagatatcca gctgcgagac gaaattctca taaccggaaa
     1921 acatcaccat caccagcaaa ccgccaacca gcgtcatatc caccagcgac agcaacacga
     1981 ggatcaaatc tgattccgcc atcgagaaga tattcggcag tacgtgaata atctcctgga
     2041 agaacttcag cgccagggca actaacgcca gcgaaaggcc aaagtacacg ggggcaagca
     2101 gccagcgaga agcatacatt gcattttcaa gaaaacgttc catacattcc tgtcgtgtag
     2161 ttaaaacggg caacagtata tcgcaattga gtaaacagca cgcaacagca aatcaacata
     2221 tacgttaaac ctttgtcgca taattctgag gtttagggat cttcctggtt cccttatcaa
     2281 cgcatcgaga gcgtctaccc ttaaaagacg ttgaacctga agggagaaaa acaatgtctc
     2341 atttaaaaga cccgactacg cagtattaca ctggtgaata tcccaaacag aaacaaccga
     2401 cgccaggcat ccaggcgaag atgacaccgg taccggattg cggcgagaaa acctatgttg
     2461 gtagcggtcg cctgaaagat cgtaaagcac tggtgacagg gggcgattcc ggaataggtc
     2521 gcgctgccgc catcgcttac gcgcgtgaag gggctgacgt ggcgatcagt tatcttcccg
     2581 tggaagaaga agacgctcag gatgtgaaaa agatcattga agaatgcgga cgcaaagccg
     2641 ttctgctgcc aggcgattta agcgatgaga aattcgcccg ttcgctggtt cacgaagcgc
     2701 acaaggcgtt aggcgggctg gatattatgg cgctggtcgc cgggaaacag gttgccattc
     2761 cggatattgc agacctcacc agcgaacagt ttcaaaagac ctttgccatt aacgttttcg
     2821 cgctgttctg gctaacccag gaagcgatcc ccctgctacc gaaaggtgca agtatcatca
     2881 ccacttcgtc aatccaggca taccagccaa gtccgcattt actggactat gcggctacga
     2941 aggcggcgat tctgaactac agccgtggcc aggcaaaaca ggtcgcggag aaaggtattc
     3001 gggtgaatat tgtcgcgcca ggccctatct ggacggcact gcaaatttcc ggcggacaaa
     3061 cgcaggataa gatcccgcag tttggtcagc aaacgccgat gaagcgtgcg gggcaaccgg
     3121 cggaactggc ccctgtatat gtttatctgg caagtcagga gtcgagctac gtcaccgcag
     3181 aagtgcacgg tgtgtgcggc ggcgagcatt taggttaaaa aaatgcccgg ttgtgaaaag
     3241 caaccgggca tcattgtgaa ttcactctta cctgatgtgg cgaaaacgcc ttatccgcct
     3301 acaaacgcat gaagattcaa catgttgcaa tagattttgt aggcctgata agcgtagcgc
     3361 atcaggcatt gagcacctaa tgccggatgc ggcgtgaacg ccttatccgg cctacaaacg
     3421 cacgaagatt caacatgttg caatagattt tgtaggcctg atgagcgtag cgcatcaggc
     3481 aattctactt tacttcgctt tggcggtttc ttcgccgacc agacctatct tcaggtaacc
     3541 cgcctgatgc agcgtatcca ttaccttcat caacgtctcg taatcgacgg ttttatccgc
     3601 tcggaagaag atggtggtgt ctttcttgcc ttcggttaac gcattcaacg ccgtaatcat
     3661 tgtttcatcg gtgaccggat cgttaccgat aaacatcgag ttgtctgcct tcaccgacag
     3721 ataaaccggt ttttccgggc gcggctgcgg cgtgctggta gaagcaggca agttcacctt
     3781 cacatctacc gtcgctaacg gtgccgccac cataaagata atcagcagaa ccaacatcac
     3841 gtcgataaac ggcgtcacgt tgatatcatg catttcgccg ttatcgtcga ggttttcgtt
     3901 aagatgcatt gccatcggat attatcctgc gcgtaatttt tgtgcgacac gaaccggatg
     3961 cgcagcggcg ctggcttcca gatccaggtc acggctttgc agcaacaata cctgcgctgc
     4021 aacatcaccc agcatcgctt taaagccgcc aatctggcgt gcaaatacgt tatagataac
     4081 gaccgcagga atcgctgcca cgaggccgat tgccgttgct aacagagctt ctgcgatacc
     4141 cggcgcaacg actgccaggt tagtggtctg cgtttgcgcg ataccaataa agctgttcat
     4201 gatgccccag accgtaccaa acagaccaac gaacggagaa atcgcgccga tggttgccag
     4261 atagccgtta ccgcggccca tttgacgacc cactgcggcg acccgacgct ccagacggaa
     4321 gctggtacgt tctttaatac cttcgttatc gtcgctgcct tctgacagtt ccagctcatt
     4381 ctgcgcttca ttgaacaaat gcaggcttaa gcttttgcta ccaaaatcag cggcgatatc
     4441 gttggcctgg tttaaggaac gcgcttcagc cagcagttgc tgctcgcgct ttaggcgacg
     4501 cttctgattg aagaactcta cgctcttact gaagaagatt gcccaggtga ctacggaggc
     4561 caaaataagc ccaatcatca cgcacttaac gacaatatcg gcgtgctgat acatacccca
     4621 gacggaaagg tctgtctgca ttaaattatt acccactgtg tatctccagg acgcaagtca
     4681 caaaatctgc gcataataat atcaaaacga cgtcgaattg atagtcattc tcattactat
     4741 ttgcatactg ccgtaccttt gctttctttt ccttgcgttt acgcagtaaa aaagtcacca
     4801 gcacgccatt tgcgaaaatt ttctgcttta tgccaattct tcaggatgcg cccgcgaata
     4861 ttcatgctag tttagacatc cagacgtata aaaacaggaa tcccgacatg gcggacaaaa
     4921 agcttgatac tcaactggtg aatgcaggac gcagcaaaaa atacactctt ggcgcggtaa
     4981 atagcgtgat tcagcgcgct tcttcgctgg tatttgagag tatggaagcc aaaaagcacg
     5041 cgacgcgcaa tcgcgccaat ggcgagttgt tctatggacg gcgcggaacg ttaacccatt
     5101 tttccttaca acaagcgatg tgtgaactgg aaggtggcgc aggctgcgcg ctatttccct
     5161 gcggggcggc agcggttgct aattcgattc ttgcttttgt cgaacagggc gatcatgtgt
     5221 tgatgaccaa cactgcctat gaaccgagtc aggatttctg tagcaaaatc ctcagcaaac
     5281 tgggcgtaac gacatcgtgg tttgatccgc tgattggtgc cgatatcgtt aagcatctgc
     5341 agccaaacac taaaatcgtg tttctggaat cgccaggctc catcaccatg gaagtccacg
     5401 acgttccggc gattgttgcc gccgtacgca gtgtggtgcc ggatgccatc attatgatcg
     5461 acaacacctg ggcagccggt gtgctgttta aggcactgga ttttggcatc gatgtttcta
     5521 ttcaagccgc caccaaatat ctggttgggc attcagatgc gatgattggc actgccgtgt
     5581 gcaatgcccg ttgctgggag cagctacggg aaaacgccta tctgatgggc cagatggtcg
     5641 atgccgatac cgcctatata accagccgtg gcctgcgcac attaggtgtg cgtttgcgtc
     5701 aacatcatga aagcagtctg aaagtggctg aatggctggc agaacatccg caagtagcgc
     5761 gagttaacca ccctgctctg cctggcagta aaggacacga attctggaaa cgagacttta
     5821 caggtagcag cgggctgttt tcctttgtgc ttaagaaaaa actcagtaat gaagagctgg
     5881 cgaactatct ggataacttc agtttattca gcatggccta ctcgtggggc gggtatgaat
     5941 cgttgatcct ggcgaatcaa ccagaacata tcgccgccat tcgcccacaa ggcgagatcg
     6001 attttagcgg gaccttgatt cgcctgcata ttggtctgga agatgtcgac gatctgattg
     6061 ccgatctgga cgccggtttt gcgcgaattg tataacattg ccacttttgg acaattttgc
     6121 agacatttaa ttgtgaaaag tcttaaattg ttgcgtccgg gatcaaggcg tcccggacga
     6181 atcaggagta caataggcag ataaaggctt aaacgctgtt ccacaggaaa gtccatggct
     6241 gttattcaag atatcatcgc tgcgctctgg caacacgact ttgccgcgct ggcggatcct
     6301 catattgtta gcgttgttta ctttgtcatg tttgccacgc tgtttttaga aaacggcctg
     6361 ctgcccgcct catttttgcc aggcgacagc ttgttgatac tggcaggcgc attgattgcc
     6421 cagggggtta tggattttct gcctacgatt gcgattctga ccgccgcagc aagtctgggc
     6481 tgctggctaa gttatattca ggggcgctgg ttagggaata ccaaaacggt gaaaggctgg
     6541 ctggcacagc ttcctgctaa atatcaccag cgcgccacct gcatgtttga ccgccacggt
     6601 ctgctggcgc tgctggctgg acgttttctt gcatttgtcc gtacgctgct gccaaccatg
     6661 gcgggaattt ccggtctgcc aaaccgccgc ttccagtttt tcaactggtt aagtggattg
     6721 ctgtgggtca gcgtggtaac cagttttggc tatgccttaa gtatgattcc gttcgttaaa
     6781 cgccatgaag atcaggtaat gacgttcctg atgatcctgc caattgcctt gttaaccgct
     6841 ggcttgttag gcatgctgtt tgtggtgatt aaaaaaaaat actgtaacgc ctgacgattt
     6901 tccccgttcc cggctgctgt accgggaacg tatttaattc ccctgcatcg cccgcattct
     6961 tgccgcatct tcccccggcg tcacaccgaa gtaacgttta aactcacggc taaattgcga
     7021 tgcgctttca tagccgacgc gcatcgctgc tgcgctggcc ttcatgccgt catggatgat
     7081 catcatccgc gccttatgca gacggtaatt cttcaaatac tgcaacggcg aggtgctggt
     7141 gacagactta aaattatggt ggaacgccga tacgctcatg ttggcttctg ccgccagttg
     7201 ctcgacgctc aggttttcgg tgtatttatt ctcaatccgt ttcagcacgc ggctaatcag
     7261 actgaagtga gtctggcgac tgaccagcgc cagtaacgcg ccgccgcaag gtccggtcag
     7321 cacgtagtac agaatttcgc ggatgatctg tttgccgaga atacgcgcat ccagtggtcg
     7381 ctccatcacg tcgagtaacc gctccgccgc gcataaaatc tcttctgata acgtggcgga
     7441 gttaatcccg ctggctgcca tcgacggctg gaaatgctca tcttcgccaa tgtccatcaa
     7501 cagttcctgt aactgcaaaa tatcgacatt gagacgcaac cctgccagcg gcacctctga
     7561 cgtggcatag gtttcgcact caaacggcaa cggcaccgtc agcagcaggt attcattggc
     7621 atcataacga aacacgcgtt cattgatata accgatttta tgcccggaaa agagaattat
     7681 gatgccaggc tcgtacatca ccggtgtacg tgcgaaaggc gtctcaccat acaacaaacg
     7741 cacatcgggc aacagttctg acaaactatt ttctttattt ttcagtttat taactttatc
     7801 cgccagcaag cggcaaatct cttcacgttt catatcgcgt aatttcttag gaataatgcg
     7861 gcaatttgat tgtgcgcaat tttgtagcat ttctccagca ctctggagaa ataggcaaga
     7921 cattggcaga aatgagcatt gagagccagg gcgctggcga tcacaatgaa aaacatcagg
     7981 cagatcgttc tctgccctca tattggccca gcaaagggag caagtaatga acaactttaa
     8041 tctgcacacc ccaacccgca ttctgtttgg taaaggcgca atcgctggtt tacgcgaaca
     8101 aattcctcac gatgctcgcg tattgattac ctacggtggc ggcagcgtga aaaaaaccgg
     8161 cgttctcgat caagttctgg atgccctgaa aggcatggac gtgctggaat ttggcggtat
     8221 tgagccaaac ccggcttatg aaacgctgat gaacgccgtg aaactggttc gcgaacagaa
     8281 agtgactttc ctgctggcgg ttggcggcgg ttctgtactg gacggcacca aatttatcgc
     8341 cgcagcggat aactatccgg aaaatatcga tccgtggcac attctgcaaa cgggcggtaa
     8401 agagattaaa agcgccatcc cgatgggctg tgtgctgacg ctgccagcaa ccggttcaga
     8461 atccaacgca ggcgcggtga tctcccgtaa aaccactggc gacaagcagg cgttccattc
     8521 tgcccatgtt cagccggtat ttgccgtgct cgatccggtt tatacctaca ccctgccgcc
     8581 gcgtcaggtg gctaacggcg tagtggacgc ctttgtacac accgtggaac agtatgttac
     8641 caaaccggtt gatgccaaaa ttcaggaccg tttcgcagaa ggcattttgc tgacgctgat
     8701 cgaagatggt ccgaaagccc tgaaagagcc agaaaactac gatgtgcgcg ccaacgtcat
     8761 gtgggcggcg acgcaggcgc tgaacggttt gattggcgct ggcgtaccgc aggactgggc
     8821 aacgcatatg ctgggccacg aactgactgc gatgcacggt ctggatcacg cgcaaacact
     8881 ggctatcgtc ctgcctgcac tgtggaatga aaaacgcgat accaagcgcg ctaagctgct
     8941 gcaatatgct gaacgcgtct ggaacatcac tgaaggttcc gacgatgagc gtattgacgc
     9001 cgcgattgcc gcaacccgca atttctttga gcaattaggc gtgccgaccc acctctccga
     9061 ctacggtctg gacggcagct ccatcccggc tttgctgaaa aaactggaag agcacggcat
     9121 gacccaactg ggcgaaaatc atgacattac gttggatgtc agccgccgta tatacgaagc
     9181 cgcccgctaa gctttttacg cctcaaactt tcgttttcgg gcatttcgtc cagacttaag
     9241 ttcacaacac ctcaccggag cctgctccgg tgagttcata taaaggagga acgtatggct
     9301 aatccaaccg ttattaagct acaggatggc aatgtcatgc cccagctggg actgggcgtc
     9361 tggcaagcaa gtaatgagga agtaatcatc gccattcaaa aagcgttaga agtgggttat
     9421 cgctcgattg ataccgccgc ggcctacaag aacgaagaag gtgtcggcaa agccctgaaa
     9481 aatgcctcag tcaacagaga agaactgttc atcaccacta agctgtggaa cgacgaccac
     9541 aagcgccccc gcgaagccct gctcgacagc ctgaaaaaac tccagcttga ttatatcgac
     9601 ctctacttaa tgcactggcc cgttcccgct atcgaccatt atgtcgaagc atggaaaggc
     9661 atgatcgaat tgcaaaaaga gggattaatc aaaagcatcg gcgtgtgcaa cttccagatc
     9721 catcacctgc aacgcctgat tgatgaaact ggcgtgacgc ctgtgataaa ccagatcgaa
     9781 cttcatccgc tgatgcaaca acgccagcta cacgcctgga acgcgacaca caaaatccag
     9841 accgaatcct ggagcccatt agcgcaagga gggaaaggcg ttttcgatca gaaagtcatt
     9901 cgcgatctgg cagataaata cggcaaaacc ccggcgcaga ttgttatccg ctggcatctg
     9961 gatagcggcc tggtggtgat cccgaaatcg gtcacacctt cacgtattgc cgaaaacttt
    10021 gatgtctggg atttccgtct cgacaaagac gaactcggcg aaattgcaaa actcgatcag
    10081 ggcaagcgtc tcggtcccga tcctgaccag ttcggcggct aacatgcaaa ttctcccggt
    10141 ggcggtaatg ttccgctacc ggacttttca gaaatcattt attcccctcg cgtcccgccc
    10201 gttgttactc ttccttgttc aggaatgcca aatataagga catcatcatg cagagccgga
    10261 agctcttaaa agaacaactc atctatatcc gggataaacg caacggagag gtgaaaaaca
    10321 gatgaaaata atacttctgt ttttagcagc cctggcaagt tttaccgtac acgcgcagcc
    10381 tccttcacag accgtagaac aaacagtccg gcagatttat cagaactata aatcagatgc
    10441 cagtacgcct tatttcggtg aaaccggaga gcgggcgata acttcagcgc gtattcaaca
    10501 ggcgcttacc ctgaacgaca atcttacgct gccgggcaat attggctggc tggattatga
    10561 tccggtttgt gattgtcagg attttggcga tctggtgcta gaaagcgttg cgataaccca
    10621 aactgacgcc gatcatgccg atgccgttgt gcgctttcgt atctttaaag atgataaaga
    10681 aaagaccacg cagacactga aaatggtggc ggaaaatggt cgttgggtca ttgacgatat
    10741 tgtcagcaat catggcagtg tcttacaagc agttaatagc gagaatgaaa aaacgctggc
    10801 cgctttagct tcgttgcaaa aagaacagcc ggaagccttt gttgccgaac tctttgaaca
    10861 tattgctgat tatagctggc cgtggacgtg ggtggtttcc gactcttacc gccaggcggt
    10921 taatgccttc tataaaacca ccttcaagac ggccaataat cccgatgaag atatgcaaat
    10981 agaacggcaa tttatttacg acaatccgat ctgttttggc gaagagtcgc tattttcacg
    11041 cgttgatgaa attcgagtcc tggagaaaac cgccgattcc gcccgcattc atgttcgttt
    11101 tacgctgacc aatggcaaca acgaagagca agaactggtt ttacagcggc gcgaaggcaa
    11161 gtgggaaatc gctgatttta tccgcccgaa cagcggcagc ctacttaagc agattgaggc
    11221 aaaaactgcc gccagattaa agcaatgagc tgaattaaat aacaattagc cggaacaata
    11281 aataaaaggg aacactatat gaaaacgatt ttcaccgtgg gagctgttgt tctggcaacc
    11341 tgcttgctca gtggctgcgt caatgagcaa aaggtcaatc agctggcgag caatgtgcaa
    11401 acattaaatg ccaaaatcgc ccggcttgag caggatatga aagcactacg cccacaaatc
    11461 tatgctgcca aatccgaagc taacagagcc aatacgcgtc ttgatgcaca ggactatttt
    11521 gattgcctgc gctgcttgcg tatgtacgca gaatgataaa aaaatccccg gcagcatgtc
    11581 agttgccggg ttataggctg tttgctaaaa aaaagccaaa cataaggaca tgattatgcg
    11641 acgctggcac acctgttttt ttattctgct gttctgtggc ttcagcggaa acgtgttgtg
    11701 caaagaatcg attaatgaag cgatagaacg tttgtaccaa ccattgacca gtaatgacga
    11761 tataccaata accgatgaat tcagtctcga tttgcgaagt aatatgagcg ctactcctgg
    11821 caaaatgaca ccctggctga ataataatct tttgtgtcgc tgtgtcgatg acgctttcgt
    11881 ttctgcagaa ataatccaaa gcacacacct aaatggcggc cgaacgcatg tactgttgca
    11941 gctggttatc aaacaaaatc gcacagatat ccaccggtta ctggggctaa tgctggttcg
    12001 tgaaaatgat aatcatcgtt ggctaataga agacatcgat gac
//
LOCUS       DAZPEB010000125        11948 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZPEB010000125 DAZPEB010000000
VERSION     DAZPEB010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11948
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.126"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..2521
                     /gene="nifJ"
                     /locus_tag="ACHURJ_002905"
     CDS             <1..2521
                     /gene="nifJ"
                     /locus_tag="ACHURJ_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFR1281779.1"
                     /translation="LYLDVMTALAEAFNNGKRETLPRVIGGRYGLSSKEFGPDCVLAV
                     FAELNAAKPKARFTVGIYDDVTNLSLPLPENTQPNSAKLEALFYGLGSDGSVSATKNN
                     IKIIGNSTPWYAQGYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFI
                     DKYQMAERLKPGGIFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECG
                     LAARINTVMQMAFFHLTQILPGDSALAELQGAIAKSYSSKGQDLVEGNWQALALARES
                     VEEVPLQPVNPHSANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGT
                     TRWEKRNIAEEIPIWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVK
                     SRDMRGQKYVLQVAPEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYD
                     FFLNLPEIDRSKLERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIAN
                     ATGCSSIYGGNLPSTPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLL
                     DQFADKIPAELLTALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWL
                     IGGDGWAYDIGFGGLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHG
                     KRKARKDLGVSMMMYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHG
                     YDLALSHDQMRQLTATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFR
                     RLNSQQPEVAEQLWKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            2887..4020
                     /gene="ompN"
                     /locus_tag="ACHURJ_002906"
     CDS             2887..4020
                     /gene="ompN"
                     /locus_tag="ACHURJ_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HFR1281780.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGFSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            4161..4595
                     /gene="uspF"
                     /locus_tag="ACHURJ_002907"
     CDS             4161..4595
                     /gene="uspF"
                     /locus_tag="ACHURJ_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFR1281781.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            5152..6066
                     /gene="sitA"
                     /locus_tag="ACHURJ_002908"
     CDS             5152..6066
                     /gene="sitA"
                     /locus_tag="ACHURJ_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HFR1281782.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            6066..6893
                     /gene="sitB"
                     /locus_tag="ACHURJ_002909"
     CDS             6066..6893
                     /gene="sitB"
                     /locus_tag="ACHURJ_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HFR1281783.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGYMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            6890..7747
                     /gene="sitC"
                     /locus_tag="ACHURJ_002910"
     CDS             6890..7747
                     /gene="sitC"
                     /locus_tag="ACHURJ_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HFR1281784.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            7744..8601
                     /gene="sitD"
                     /locus_tag="ACHURJ_002911"
     CDS             7744..8601
                     /gene="sitD"
                     /locus_tag="ACHURJ_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HFR1281785.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(8939..9712)
                     /locus_tag="ACHURJ_002912"
                     /pseudo
     CDS             complement(8939..9712)
                     /locus_tag="ACHURJ_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISSfl3 family transposase"
     gene            complement(9718..10230)
                     /locus_tag="ACHURJ_002913"
                     /pseudo
     CDS             complement(9718..10230)
                     /locus_tag="ACHURJ_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
     gene            complement(10269..10556)
                     /locus_tag="ACHURJ_002914"
     CDS             complement(10269..10556)
                     /locus_tag="ACHURJ_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1281786.1"
                     /translation="MKIEELREIFSENGLYAVRVENGAIVSHCRIRCLQSQQRKSGAV
                     LFYFCNGLLTDGFILREDEFVTSLRVLKEIGFKAGFLLLLKNKLIYNLEQD"
     gene            complement(10806..10961)
                     /gene="hokD"
                     /locus_tag="ACHURJ_002915"
     CDS             complement(10806..10961)
                     /gene="hokD"
                     /locus_tag="ACHURJ_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR1281787.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            complement(11189..11854)
                     /locus_tag="ACHURJ_002916"
     CDS             complement(11189..11854)
                     /locus_tag="ACHURJ_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFR1281788.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
BASE COUNT         2972 a         2982 c         2871 g         3123 t
ORIGIN      
        1 gctctatctg gatgtgatga ccgcactggc agaagccttt aataatggca agcgcgaaac
       61 tctgccccgt gtcattggtg ggcgctatgg tctttcatcc aaagaatttg gcccggactg
      121 tgtactggcg gtatttgccg agctcaacgc ggctaaaccg aaagcgcgct ttacggttgg
      181 tatttacgat gatgtaacca atctgtccct gccgttgccg gaaaacaccc agccaaactc
      241 ggcgaaactt gaagccttgt tttatggcct tggtagtgat ggcagcgttt ccgcgaccaa
      301 aaacaatatc aagattatcg gtaattccac gccgtggtac gcacagggct attttgttta
      361 cgactccaaa aaagcgggcg gcctgacggt ttctcacctt cgcgtgagcg aacagccgat
      421 tcgttccgct tatctcattt cccaggctga ttttgttggc tgccaccagt tgcagtttat
      481 cgataaatat cagatggctg agcgtttaaa acctggcggc attttcctgc tcaacacgcc
      541 gtacagcgca gatgaagtgt ggtcgcgctt gccgcaagaa gttcaggccg tgttaaacca
      601 gaaaaaagcg cgcttctatg tgattaacgc ggcgaaaatc gcccgcgaat gtggcctggc
      661 ggcccgtatt aataccgtca tgcagatggc ttttttccat ctgacgcaaa ttctgcctgg
      721 cgatagcgcc ctcgcagaat tgcagggtgc gattgccaaa agttacagta gcaaaggcca
      781 ggatctggtg gaaggcaact ggcaggctct ggcgctggcg cgtgaatccg tagaagaagt
      841 tccgttgcaa ccggtaaatc cgcacagcgc caatcgaccg ccagtggttt ccgatgccgc
      901 ccctgatttc gtgaaaaccg taaccgcagc gatgctcgca gggcttggtg atgccctccc
      961 cgtttcggcg ctgccgccag acggcacctg gccgatgggg accactcgct gggaaaaacg
     1021 caatatcgcc gaagagatcc ccatctggaa agaggaactc tgtacccaat gtaaccactg
     1081 cgtcgccgct tgcccacact cagctattcg cgcaaaagtg gtaccacccg aagcgatgga
     1141 aaacgcccct gccagcctgc attcgctgga tgtgaaatcg cgtgatatgc gcgggcagaa
     1201 atatgtcttg caggtggctc cggaagattg caccggttgt aacctgtgcg tcgaagtttg
     1261 cccggcgaaa gaccgtcaga atccagagat taaagccatc aatatgatgt cgcgcctgga
     1321 acatgtcgaa gaagagaaaa tcaattacga tttcttcctc aacctgccag aaatcgaccg
     1381 tagcaaactg gaacgtattg atattcgtac atcgcagctg attacaccgc tgtttgaata
     1441 ttccggtgct tgctccggtt gtggcgagac gccgtatatt aaattgctga ctcagctcta
     1501 tggcgaccgg atgttgatcg ctaacgccac cggctgttct tcaatttatg gcggtaacct
     1561 gccctctaca ccgtatacca ccgatgccaa cggtcgtggg ccggcatggg cgaactctct
     1621 atttgaagat aatgccgaat ttggccttgg tttccgcctg acggtcgatc aacaccgtgt
     1681 ccgcgtgctg cgtctgctgg atcaatttgc cgataaaatc ccggcggaat tactgacggc
     1741 gttgaaatca gacgctacgc cagaggttcg tcgagaacag gtcgcagctt tacgccagca
     1801 actcaacgat gttgccgaag cacatgaact gctacgtgat gcagatgcac tggtggaaaa
     1861 atcaatctgg ctgattggtg gtgatggctg ggcttacgat atcggctttg gcggtctgga
     1921 tcatgtattg agtttgacgg aaaacgtcaa cattctggtg ctggatacgc aatgctattc
     1981 caacaccggt ggtcaggcgt cgaaagcgac accgctgggt gcagtaacta aatttggcga
     2041 gcacggcaaa cgtaaagcgc gtaaagatct tggcgtcagt atgatgatgt acggtcatgt
     2101 ttatgtggcg cagatttctc tcggcgcgca gctgaatcag acggtgaaag cgattcagga
     2161 agcggaagcg tatccgggac catcgctgat cattgcttat agcccgtgtg aagagcatgg
     2221 ttacgatctg gcactcagcc atgaccagat gcgccaactc acagctaccg gcttctggcc
     2281 gctatatcgc ttcgatcctc gtcgtgccga tgaaggcaaa ctgccgctgg cgctggattc
     2341 acgcccgccg tcagaagcac tggaagaaac gttacttcac gagcaacgtt tccgtcgtct
     2401 gaattcgcag cagccagaag tggcagaaca gttatggaaa gatgctgcag ctgatttgca
     2461 aaaacgctat gacttcctgg cacaaatggc cggaaaagcg gaaaaaagca acaccgatta
     2521 aatgctctgg ataaggatta tccaattcta aaaaaaagcc cggacgactg ttcgggcttg
     2581 tctttttata tgttggaaaa tcagtggcaa tgcaatggcc caacagaaaa taaaatctgt
     2641 gacaataaag gcatataacc cgcgcagaat aacgtataga aaattaaaag tataattttt
     2701 attttgtatg tattatcttc gatacattca ccagacttat tatcatttca gaagaattat
     2761 ttgattaagg ttttacttaa ggcgtaacaa atgatattta tcggctaact gaacttctcc
     2821 tttaggatgt tttcactccc cggaatgggg ataaaaggca aataaaataa ctaaaggatt
     2881 tattcaatga aaagcaaagt actggcactt ttaattcctg ccctgctcgc cgcaggtgct
     2941 gcacatgcag ccgaagttta taataaagac ggcaacaaat tagatctgta tggcaaagtt
     3001 gatggcctgc attatttttc tgataattca gcgaaagatg gcgatcagag ctatgcgcgt
     3061 ctgggtttta aaggcgaaac ccaaattaac gatcaactca ctggctacgg tcaatgggaa
     3121 tacaatattc aagcaaacaa cactgaatct tcaaaaaacc agtcatggac ccgtctggca
     3181 tttgccgggc tgaaatttgc agattacggt tctttcgatt acggacgtaa ttatggcgta
     3241 atgtacgaca tcgaaggctg gaccgatatg ctgcctgaat ttggcggtga ctcttatacc
     3301 aatgcagaca actttatgac tggtcgagcc aatggcgtcg cgacttatcg taatactgat
     3361 ttcttcggtc tggtaaatgg tctgaacttc gcggtgcagt atcaaggtaa caacgaagga
     3421 gccagtaatg gtcaggaagg caccaacaac ggacgtgatg ttcgccatga aaacggtgac
     3481 ggctggggtt tttccacaac atatgattta ggcatgggct ttagcgctgg tgcggcatac
     3541 acctcttctg accgcaccaa tgaccaggtt aaccatactg cggcgggtgg tgataaagca
     3601 gacgcgtgga ctgctgggct aaaatacgat gctaacaata tttacctggc aaccatgtat
     3661 tcagaaacgc gtaatatgac cccgtttggc gacagcgatt atgctgtggc aaacaaaacc
     3721 cagaattttg aagtcactgc ccagtaccag tttgattttg gtctgcgtcc ggcagtctct
     3781 ttcctgatgt ctaaaggccg tgacctgcac gctgccggtg gtgcagacaa cccggcaggt
     3841 gttgatgata aagatctggt taaatacgcc gatgttggcg cgacttacta tttcaataaa
     3901 aacatgtcca cctacgttga ctataaaatc aacctgttgg atgaagatga cagcttctac
     3961 gctgccaatg gcatctctac cgatgatatt gtcgctttag gtctggttta tcagttctaa
     4021 atcctcctgc ccgctgttat ggcgggcttt ttctgcttat tcttcctcct ttgatctaaa
     4081 ttaaaaatgt gaactccatc attacacaaa aagtgtcatc tggcgttaca ctttatgcgg
     4141 atactaaaac aggaggtttt atgaacagaa cgattcttgt ccctatcgat atttccgatt
     4201 cagaattaac tcaacgcgtg attagccacg ttgaggcaga ggcaaagatt gacgatgcag
     4261 aggttcattt cctgacggta ataccttcac tgccctacta tgcctctctg ggtttagcgt
     4321 attccgcaga attaccggca atggatgacc tgaaagcgga agccaaatcg caactggaag
     4381 agatcattaa aaaatttaaa ctgccaaccg acagagtgca tgtccatgtt gaggaaggct
     4441 cgcccaaaga ccgcattctg gaattggcga agaagatccc cgctcatatg atcatcattg
     4501 cttcccatcg accggatatc accacttatc tgctcggttc caacgccgca gctgtagtgc
     4561 gtcacgcaga gtgctccgtg ctggttgtgc gctgacacta acgcccgcac attgctgcgg
     4621 gctttttgat tcatttcgca aatgtgctga cattttcccc tctaatccgt accatacacg
     4681 ccactgtttt tatatcagat ttcttatgct gggcattccg gcatgacgca tactcttctg
     4741 atgccatata acgaattgag tcgcttttaa atgtcgcaaa atcaagaaat tagtaagaaa
     4801 gaacaataca acctgaacaa gtaagggcaa aaatcacaac tatctgactc ataagtattt
     4861 tatttatttt tcaatatgtt aaacattatc agcgacaaca ataagctacg ataacccact
     4921 ctttttttgc cccatttttg ccatcaacaa ttttcctcat aaagtcattt caaagcctaa
     4981 tagctgctct acttttttcc tcaatatcat gtcgccagca ggaaacaacc taatccctgt
     5041 tttaggcctc aaagctgtta atgataatta ttttcatttt gtataggtta tgctataaaa
     5101 catagccgtt gctatgtttg ttctaatttt gctcactata ggtactaaat tatgcactca
     5161 ataaaaaaag taaccatgct cttggggtgc ctcgccctca cctgctcgat cgcatttcag
     5221 gcaagtgcaa ctgaaaaatt caaggtcatt acaacattta ccatcatcgc ggatatggcc
     5281 aaaaacgtgg ctggagatgc tgcagaagtc tcatccataa ccaagcctgg tgcagaaatt
     5341 catgagtatc agcctacccc tggcgatatt aaacgtgcac agggggcaca actgattctc
     5401 gccaatggta tgaatctgga attgtggttc caacgctttt accagcatct caatggggtt
     5461 ccagaagtaa ttgtctcttc gggtgtgacg ccagtaggga tcaccgaagg accctatgag
     5521 ggcaaaccta atccccatgc atggatgtcg ccagataatg ctctgattta cgtcgataat
     5581 attcgtgatg cgttgataaa atacgatccg gcaaatgcac aaacctacca acgtaatgcc
     5641 gatacttata aagccaagat tacccaaacc cttgcccccc tgcgtaagca gattacggaa
     5701 ctccctgaga atcagcgatg gatggtcacc agtgaagggg ctttttctta tctcgcacgc
     5761 gatttggggc taaaagagct ttatctgtgg ccgattaatg ccgatcaaca aggaacaccg
     5821 cagcaggtac gtaaggttgt tgatatagtt aagaaaaatc atatcccggc agtctttagc
     5881 gagagtacga tttccgataa accagcgcgt caggttgcgc gtgaaaccgg cgcacactac
     5941 ggcggtgtgc tctatgtcga ttccctgagc acagaaaacg gcccggtacc tacgtatatc
     6001 gaccttctta aggttactac cagtacgctg gtacagggaa ttaaagccgg gaaaagggag
     6061 aaataatgat gcaatctgca ggcattgtcg ttaatgatgt tactgtcacc tggcgtaacg
     6121 ggcacacagc actgcgcgat gcatccttca cagtaccaag tggatctatt gccgctctgg
     6181 ttggggtaaa cggttccgga aaatcgacgt tgtttaaagc gattatggga tttgtgcgtc
     6241 tgaccagcgg aaagatatct gttctgggta ttcccacgcg acaggcgcta cagaaaaacc
     6301 tggttgccta cgtccctcag tcagaagagg ttgactggtc atttcctgta ctggtggaag
     6361 atgtggtgat gatgggccgc tatgggtata tgggtatgtt gcgtatagcc aaaaagaggg
     6421 atcgtcagat tgtcaccgat gcgctggaac gtgtcgatat ggtggatttt cgccatcgtc
     6481 aaatcggcga gctttccggt ggacaaaaga aacgcgtctt tctggcgaga gcgattgcac
     6541 agcagggtga tgtgatcctg ctggatgaac cttttaccgg cgttgatgtg aaaaccgaag
     6601 cgaaaatcat cagcctgttg agggagttac gcgcggaagg caaaacgatg cttgtctcaa
     6661 cgcacaatct cggctcagtc acgacatttt gcgattatac ggtcatggtc aaaggcaccg
     6721 tcctggcaag cgggccgaca gacaccactt ttacagccga gaacctggag ctggctttta
     6781 gcggcgtact gcgccatgtc acacttaacg gctcggaaga aagcattatt actgacgatg
     6841 aacgcccttt tgtggcacat cgaccgtcag cggtgcagag ggaagcaaga tgaacgtgct
     6901 actggaaccc ttcagctatg aatatatgct caatgcgatg tgggtctcgg caatggtcgg
     6961 cggtctctgc gctttcctgt catgctatct gatgctaaaa ggctggtcgc tgattggcga
     7021 tgcgctgtcg cactctattg tcccgggtgt tgcgggggca tatatgctgg ggcttccgtt
     7081 ttctctggga gccttctttt ccggcggact ggcagcgggc agcatgctgt ttcttaacca
     7141 acgcacccgc ttaaaagaag atgccatcat cggcctgatc ttctcgtcct tttttggcct
     7201 ggggctgttt atggtgtcat tgaacccgac atccgtgaac attcagacca tcgtcctggg
     7261 caatattctg gcaatcgatc cggcggatat cctgcaactc actatcattg gcattctctc
     7321 gatcatcgtg ctttttttca aatggaaaga tcttatggtg acctttttcg acgagaatca
     7381 cgcccgtgct attggattac atccgggcag attaaaaatc ctcttcttca ccctgctgtc
     7441 ggtttcgacc gttgcggcgc tgcaaacggt tggtgccttt ctggtgatct gtctggtggt
     7501 gactcccggt gcaacagcat ggttgttaac cgatcgtttt ccccgcctgc tcataattgc
     7561 tgtaactatt ggcagtgtaa ccagtttcct cggcgcgtgg gtcagttact ttctggacgg
     7621 cgcgacaggt ggaatcatcg tggtggcgca gacactgctg tttcttttag catttgtatt
     7681 tgcgcccacc catggacttt tggcaaatcg tcggcgtgcg cataaagcgc tggaggatcg
     7741 ctcatgatgg cattattact ggagccactg cagttcacct ttatgagtca cgcactgctg
     7801 atttcactgg tagtttccat accctgtgcg ctgctgtcgg tctttctggt cctgaaaggt
     7861 tgggcactga tgggtgacgc gatgagtcat gcggtttttc ctggcatcgt gcttacctgg
     7921 attctggggt tacctctggc tacaggggca ttcgtcgccg gagtattctg cgcggtggcg
     7981 acgggatacc tgaaagacaa cagccgaatc aagcaagata cagttatggg gattgttttt
     8041 tctggcatgt ttgccgcagg tctgatcttg tatattgcaa tcaaaccaga cgtacatctt
     8101 gaccatattc ttttcggcga catgctcggg ataaccatcg gcgatataat ccagacggtg
     8161 attattgccg ggctggttac acttgttatt agcgtaaaat ggcgggattt tttgctgttc
     8221 agctttgatt atcaacaggc gcaggtaagc ggtttgcata cgagatggct gcattatggg
     8281 ctgctgtgca tggtttccct gaccattgtg gcgacgctga aagcagtagg tatcattctc
     8341 tctatttctc tgctcattgc gcctggcgcc attgcagtac tcctgacaca acgctttcat
     8401 attgcgctcc tactggcgac tggcatatcg gtaatagtgt caatgactgg tgtctggctg
     8461 tcctttttta tcgacagcgc accagctccg acgattgtcg tcttattcgc ggtcgtgttc
     8521 atcatgacgt ttaccgttac cagcatcaac gcacgcacaa agggaaacgc ccatacacag
     8581 gatctgttat cacccaatta gcacaatatc cgaggacagc atcgtccgcc agcgtccgcc
     8641 agcgtccgcc agcctacaga agttcggaaa gccatggaag gtcgttggga ggaggtacga
     8701 attctatcat gcaaaaaata cgtaaaatcg ataacgccta gaaatcattc aatactcgca
     8761 ctatcgaaag tttaccagcc aaccgcggca cgttcctgca tacgacgtgc cgcagcttct
     8821 gttattccac gataaaattc ctaaccgaat tcaaatcctt cagactattc agctcttcct
     8881 tcagctcccg ctgacggcga taaatcgtaa gcgccccgag aagtacgtag cgtaaggatt
     8941 attttacaga cgagaagttc cagggcagca gttcatgcac ttggttcgac ggccagtcat
     9001 tgagcttctc gatcacttcg cgcaaccagt cctccggctc cacttcgttc tgtttgcagg
     9061 tgaccagcag actgtagatg atcgccgcac tttctcctcc cttgtctgag ccgaaaaaga
     9121 gataattttt tcttccaacc gccaccgatc gtaacgcgtt ttcaccgatg ttgttgtcta
     9181 tttccaccca gccgtcacga cagaactcgt tcagcgcatt ccagtgattc aggatatagt
     9241 cgaacgcctt cgccatctcc gcatgtttcg acagcgtttt cctctgcaac tgtatccagt
     9301 cgtacaatga ctgcatcaac tggacgcttc tggcttttct gactgcaagc cgttcctctg
     9361 ccggactgcc acgtatctcc gcttctatgt cgtataactc tgctatccgt ctgagcgctt
     9421 cctgagtcat ttctgtcgga cggcgcacat cctcgtcatg gatttttcgg cgggcgtggg
     9481 ccaggcaccc ggcttccttc acccggcccg tttcgtacag tacgttataa cctgcatagg
     9541 catcagcctg cagtacgccc tgatactttg ccaggtggag ctgcggatgt tctcctttgc
     9601 gatctgccga atacgcgaac cagacggctg ccggcaggga tgaacccgca ttacgatcat
     9661 ccctgacgta tacccacaga cgacccgttt tcgtctttcc gttccccggg gcggttcatt
     9721 cagcacagat ttataaacct gctgaaactc ggtttcgtcc atattcgcaa acgagatgga
     9781 tttcgccctg cgcccacggc taccgtccgg ataaatatgc tcggtgtaaa atccggcctg
     9841 aatggttacc cactcgcgga aagcctcaaa cgactttagc aatgctgtat ctcgggttct
     9901 gcgtgtcgca actgtattca gatattgctc tgcggcttcg ctcagagctg gcgtatgttc
     9961 ccggcctact gattcgcaca ggtactcaac gaagcctgat accagttctc gttcgcgagg
    10021 cgtaatcgcc ccaccgatcg gagtccagta atcgaatccc agttgcagga gtttgaaaaa
    10081 acgcttgtgg aatgcgtagt tacgcacacg cttaaagtct gcgtgtatcc actcacctgt
    10141 tttgatttga tgcagaaaat cgcaactctc cggcgtcgcc gggagaagta atccggaaga
    10201 ggtttgtttg atcagttgta tatgcgccat cggctttctc cggtggcacg gtgttacaca
    10261 gcaggagttc aatcctgctc aagattgtag atgagtttat tcttcagcaa aagcagaaaa
    10321 ccagccttaa aaccaatctc tttcaaaacc cgtaatgatg tgacaaattc gtcctcacgc
    10381 aaaataaaac cgtccgtcag aagtccatta caaaaataaa ataacacagc accgctcttc
    10441 ctttgttgag attgcaaaca tctaatgcgg caatggctga caatcgctcc attctcaacg
    10501 cgcacagcat agaggccatt ttcactaaaa atttcacgca attcttcgat tttcatcttc
    10561 agaatccttc cagataaata gctctcccct gttcggggtc catccctctt ctccctgcgc
    10621 gctacttaag tgagtcgatt ctatctgcga aggtgcgcga atcaaattca ccggaaataa
    10681 acaacaaaaa acccgccgaa acgctgagag atcacagcga tgggcgggtt aagtgcgggt
    10741 gcgttgagga tgcctgacac atcagaggtg ggcggggatg ggatcagctc cccgcccggt
    10801 cactcttact tcctggattc gtagtctacg aagacagcga cctccgtctg gccggttcgg
    10861 attcgtacct cacagaggtc tttcctcgtt accagtgccg tcactatgac ggttaaacag
    10921 atgacgatca gggcgattaa catcgccttt tgctgcttca tagcctgctt ctccttgcct
    10981 ttcggcatgt aagaggctaa cctacatgtg caaagcatga aattggcctc agattaatgt
    11041 taagcgtctt gccggacgcg taatgttaac tggggctttt ctctatctgc cttttggtgt
    11101 tcatgcctga agcagatagc ctcaagcacc cgcagtcatt ctacttacct cgccaatatg
    11161 aaatcaatca gaaaggtgcc ccataaaatc actccttctc ttctttaccg tagtggagtt
    11221 gaccaatttt gataagaggg cgtccctgag atttgcggtg tagattggta tcgcgcagag
    11281 aatacacaca gccacaatat tcctgctgat agaatttttc gcgcttgctg atttcaatca
    11341 tacgggacga gcctccctgc ttgcgccagt tataatccca gtacaccatg cccggataat
    11401 gcgcgacggc tcgccgccca cagtcgttaa cctgctgcat atttttccag cgtgaaatgc
    11461 ccagtgaact gctgatcaca ctgaaaccat tttcagcggc gtacagcgct gtccgctcaa
    11521 aacgcatgtc aaaacacatg gtgcaacgga tccctcgttc gggctcccat tccattcctt
    11581 tggctcgttc aaaccagttg tctgtgtcgt aatcagcatc aataaacggc acgccgtgtt
    11641 gttcagcaaa gcgaatattc tcatccttac gaattaaata ctctttctga ggatgaatgt
    11701 tcgggttgta gaaaaagatg gtgtagtcga ttcccgaggc ctgaagcgcc tccatcacct
    11761 caccggaaca tggagcacag caagagtgca gtagtagttt gtttgccccg tttgggagct
    11821 ccaatttggg ccgtttgaaa tcagcaactg tcataaatat gtttattggg gtcataaaaa
    11881 tatcaaagag tgtagcatta gaacggggct atcggaaaca gatgtgtgac tgctccccgc
    11941 cctttcgg
//
LOCUS       DAZPEB010000126        11794 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZPEB010000126 DAZPEB010000000
VERSION     DAZPEB010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11794
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.127"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            486..1142
                     /gene="pphA"
                     /locus_tag="ACHURJ_002917"
     CDS             486..1142
                     /gene="pphA"
                     /locus_tag="ACHURJ_002917"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFR1281789.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVCAVRGNHEQMAMDALVSQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(1143..1334)
                     /locus_tag="ACHURJ_002918"
     CDS             complement(1143..1334)
                     /locus_tag="ACHURJ_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFR1281790.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRQGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(1439..1675)
                     /locus_tag="ACHURJ_002919"
     CDS             complement(1439..1675)
                     /locus_tag="ACHURJ_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFR1281791.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(1793..3232)
                     /gene="rsmF"
                     /locus_tag="ACHURJ_002920"
     CDS             complement(1793..3232)
                     /gene="rsmF"
                     /locus_tag="ACHURJ_002920"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFR1281792.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVDIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            complement(3312..5945)
                     /gene="yebT"
                     /locus_tag="ACHURJ_002921"
     CDS             complement(3312..5945)
                     /gene="yebT"
                     /locus_tag="ACHURJ_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFR1281793.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RSNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGTGKEQDHFVALDTQPKYRLNNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDTGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVSGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISATGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(5914..7197)
                     /gene="yebS"
                     /locus_tag="ACHURJ_002922"
     CDS             complement(5914..7197)
                     /gene="yebS"
                     /locus_tag="ACHURJ_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFR1281794.1"
                     /translation="MALNTPQITPTKKITVRSIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            7327..7824
                     /gene="msrC"
                     /locus_tag="ACHURJ_002923"
     CDS             7327..7824
                     /gene="msrC"
                     /locus_tag="ACHURJ_002923"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFR1281795.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            7921..8619
                     /gene="proQ"
                     /locus_tag="ACHURJ_002924"
     CDS             7921..8619
                     /gene="proQ"
                     /locus_tag="ACHURJ_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFR1281796.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            8639..10687
                     /gene="prc"
                     /locus_tag="ACHURJ_002925"
     CDS             8639..10687
                     /gene="prc"
                     /locus_tag="ACHURJ_002925"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFR1281797.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            10879..11760
                     /gene="htpX"
                     /locus_tag="ACHURJ_002926"
     CDS             10879..11760
                     /gene="htpX"
                     /locus_tag="ACHURJ_002926"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFR1281798.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVMN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
BASE COUNT         3033 a         2904 c         3044 g         2813 t
ORIGIN      
        1 gcaaccccaa atcgtaaagg aaaccgtagc acgtcgtatg caagaacgtg ccacggctgg
       61 ctgatggatg ttcgatagcg cgagtttgaa tgaaaatcag ccggagatga ttttacataa
      121 ttgctacgga attattcaat acaggaattg cttgcgtctg catggattga cctgaaattt
      181 tcccgaaaat ttctctaaaa aactcgaaaa aaatggtaac tggttgaatg tattaatatg
      241 caatggtacg tgtcagggat taaaagatga acgtaaattt attcaacgca ttaattttaa
      301 agggttttat tgtttgttga cgaaaacagg aatcgtgttc ggtctctttt tatctgttaa
      361 aagccagaag catttccttc gctgacttta tagtcaacca taacacacac tctactgtct
      421 gagtccagcg ttttttaaca ttcttgttaa gattatgtga tctttagcac gggaggaaaa
      481 tattgatgaa acagcctgcg cccgtttatc agagaattgc gggtcatcaa tggcgacata
      541 tctggctttc tggcgatata cacggttgtc ttgagcagtt gcgccgcaaa ttatggcatt
      601 gtcgttttga tccgtggcga gatttactta tctcagtggg agacgttatc gatcgtgggc
      661 cgcaaagttt acgttgtctg cagttactgg aacaacattg ggtttgtgcg gtaagaggca
      721 atcatgaaca gatggcgatg gatgcgctgg tatcccagca gatgtctttg tggttgatga
      781 atggcggcga ctggtttatt gcgctggcag ataatcaaca gaaacaagcg aaaacggcgc
      841 tggaaaaatg tcagcatttg ccctttattc ttgaagtaca cagtcgcacc ggcaagcatg
      901 ttattgctca tgcggattat ccagatgatg tttatgaatg gcaaaaggac gttgatttgc
      961 atcaggtctt gtggagccgc tcgcgattag gtgaacgcca aaaagggcag ggaattacag
     1021 gtgctgatca tttctggttt ggtcatacac cgttgcgaca tcgcgtggat attggcaacc
     1081 tgcattatat tgataccggt gctgtctttg ggggcgaact gactcttgtg caattgcaat
     1141 gattaaaaat caccgtactc ctgtgcaggt cgccagaaac catctataaa atcctcaatc
     1201 ggaaaacaac cgccctggcg gatccgttga tcgctcatag aataaagaca ctgctgttcc
     1261 gtgttgtaga catccacaac aatatcttca caaccgccat ccaggtagca aacaaaaagt
     1321 accagcgcga acatttcatc cccgaagtgt ggtgccgtac cgttaagttt aggagagatt
     1381 ttacaacggg ggaataacca ggacaaataa cccgccatgc cgacgggttc tttttggatc
     1441 aggcaagacg cataatccag gcatcagatt gttgatcgta acgggtacgg taaaggacag
     1501 agtgttcgcc atttagcagg gcagggatac tggtttcagc atcccaggca tcgagttgca
     1561 tacataaatc ggggtcagat ttacaaggaa tggttaacgt tcgatcaccc ggcgattcac
     1621 cgtgtaattc gccgtcgtcg atttcaaaag cgcctatgcg cacactggtt ttcatcttcg
     1681 tgctctccat ttagctgccg gttgtggtac gaccagtccg atcgcaccat ttttcagcct
     1741 agttgaggca gccgaatgtg ccagtcaaaa agtgcgcttt ttttgtgcgc tgtcaggcgt
     1801 taccggtaaa aagtttccca tcgcgcacca gttcacgcgg atagctgttt ttcaatcgcg
     1861 aaccaatccg tttggctaaa ccaatcggtt gatgctggaa agtaaccaac acgtcatccg
     1921 ccactggcgc ggcttgcggg taaacatcgc gcccgcgata ccactcctcc gcttcctgcg
     1981 gtgtcagttc aaaagcgttc acattgtcgg gggtggcaag ggcaataact gcttcatgct
     2041 gccagcgata acctttgttg tgcgtttcgg caagtttaat ccccaagcga gaaaatcgga
     2101 ctttaccgat cagggcttca atgtccaccg ggaacaacca cagttctttg tcacgctgcc
     2161 agaggcgcag gttttcatcc cagtttaagc caacacctgc agccgcctgg cgaatttgtc
     2221 cagcttcgcg atctttcacc gggctgaacg gaaaattacc gactttgtat ttgggggcgg
     2281 gtaaggcggg aatcgcctga gttttacgca gacgagcaac gaagaagcct tcgcagtcgt
     2341 aaatttgtgg gaaaacatgc aaaaagcctt cttcggtcag cgctttgttt gcaccaggga
     2401 agagatcgcc aagtggtaaa aactctactg cgtcggggta agtctctttc agccacaggc
     2461 aaacggcttc gttttcttcc tggtttaagg tacaggtcga gtaaaccagc gtaccaccag
     2521 gacgtaatgc atgaaaggcg ctgtcgataa gctcccgttg tgtagctgcg atttcctgat
     2581 tgctttctgg tgaccagttt tttagcgcat cgggatcttt acgcaccacg ccttcgccgg
     2641 agcagggagc gtccagcaaa atggcatcaa acatttctgg cactgccgca ccaaacacgc
     2701 ggccatcaaa atgtgtgagc gcaacattac tgatgccaca gcggctgata ttggcatgta
     2761 acacttttac ccgactggcg gaaaactcat tggcaaggat tgccccttcg ttattcatcc
     2821 gcgcggcaat ttgcgtcgtt tttgagcctg gcgcagcagc gacatccatc acccgctgtg
     2881 gtgcattacc gtcagcaaac aaggcggcga cgggcaacat tgaactggct tcctgaatat
     2941 aaaacaggcc acttaaatgc tcggcggtac tacccaatgg caatgcatct tcattgtcgc
     3001 gttcaatcca gaaaccttct tcacaccacg gaattggcgt aagcgtccag ccataaggag
     3061 cggttaattg caggaaatca gcaacggaga ttttcagcgt attaacgcga atgctgcggc
     3121 gcaacgggcg ctgacaggcg gcaagaaaat catcaaatga gagcgtcgaa ggcatagctt
     3181 cgcgcatttg tgtcagaaac gcgtccggga aataaacggt gtgttgggcc acgagcatgt
     3241 accactggca aaaaaacagg cgcgcagttt agcataaacg ctccggcgca ggcacgccgg
     3301 agcagtgggc attatttggg aagcgcagtt ccccattcac gccactcttt cggttcactt
     3361 tcctgcaaca ggaagtgttt gccttcctgg gctttcggtg ccagtggcgt acccggaggc
     3421 gtggcgaagg cgatgccgcc acggataaac tggttaaagg tgccggtttt cactacgccg
     3481 cccgtcagac caaagtccag actgtaaccc gatgccaacc agaagacgga attgttacgc
     3541 accaggtgtt gatagcgttt actgatgcgc atcgcaatca tcacgcgatc tgacaatgtc
     3601 cccagcgtca ttccggtaac cgtacctact tccagaccac ggaacagcac aggcgtacca
     3661 atacctaacg aaccggcttc cggtgcttcg acaataatgc ttaagccatc caggtaacgc
     3721 gaatcagtaa tagtagcttc ttgcaactca aagtcacggc gaggattacc ccgacctggt
     3781 tcgacattga tatacggctg gaggatagta tctagatgct caacgcccgt ggctgaaatt
     3841 tgcggtgtga ccaccgagaa gcgcgtaccg ccgcgggcga aggtctggac atactccgga
     3901 tagagcaccg cctttgcctg tacttcattg cgcgcggtaa tcagatccag cgtctggatt
     3961 tgcccgatat caataccgag atagcgaatt ggcatcccga ccgccagttt tccggcatcg
     4021 aaagcgtgaa gcgtaatctg cccactaacc gcacgggccg ctgtttcgga agcatacaga
     4081 attcgtttgt cgcctttacg ctgactggcg ctggcaccgc tgaggttatc gaagctaatg
     4141 gctcccttta atgctctgga gagcggggat gcctgtacgg tcaggccact accattcagc
     4201 tgaactttcg ccccgccttc tgcccagaac acgctattgc tggtcagaag gttgcgatac
     4261 tccggcttaa tatgcagatc gatatcaaac gcgttagctc gcggacgcac ggtaatcact
     4321 tcaccaactt caaatttacg gtacagcact accgatcctg cctgcacatc cggcagcgtc
     4381 tctgcactca aactcacggt tgtggtgggt aaatcgctaa ggctgttctc cagcgctttt
     4441 tccagattgg catacagtgg atagctggct ttcatctcac ctttatcgcc tggcagaata
     4501 cgtatcccgc cgttaatcca ttctgaggcg ctggcaccaa gaaactcaac gccatccagc
     4561 cccaccttca cgtcgacacg gctgttgacg acaaatttgc tatcgccttt taccagttcg
     4621 cgatgctgag gctcgatggc gacggtaaag gtaacgcctt tgctggtgag tttacgatca
     4681 ataacctggc ctacctgcac gccgtgaaga atgagcggct gacccgtatc aataccgtaa
     4741 ctttccggcg cggtcagggt cagcgtcaga acatcaggtt catgcagcag tgctttttcg
     4801 cctggcacaa caacgaactc tttgcgtggc tcgccatcgc cgggtaccaa ctcgaaggtt
     4861 ttgccagtca gcagggcgct gagattggca tcgctaaggg ataatttcgg attacgtaat
     4921 tcgatgcggg tattttcacg aagcagggta acgacgctgg gatcaactgt catttcccca
     4981 gtgactttac caccaggatt taaatccagt ttagttagct gtccgacttc cagtccctga
     5041 tacattaatg gcgtcgagtc ggcggttaat ccggccccac tcggcagttc cagtttgatt
     5101 attacgccac gctggctatg ggcgaggtct tcatacagac caaaggtatc ttccgcctcg
     5161 gcaggtttcg actcttctgg tgaatcgaag gcaatcgcac cgttaaccag tgccgccagg
     5221 ctttccagtt tcaccttcgc gccactgata ctgacattgg catcaacgcc ggaaacgttc
     5281 cagaaacggc tacctttttt caccagatcg gtaaaacgcc gctcgatcag gacatcaatc
     5341 accacgcctt gtttgttggg attgatggca tagtcgtaga cttttcccac cgggatcttg
     5401 cggaaataga ccaatgaacc gctgttcagc gaaccgagat cgggggcttg caggtggatc
     5461 atcagatcgc cattgttcag ccgatatttc ggttgggtat cgagtgcgac aaagtgatcc
     5521 tgctctttac ctgtacccgg catcatgccg atatagttcc caccgacgag ggcgtccagc
     5581 ccggagacac ctgccaacga tgcttttggc gtcaccagcc agaattgagt ctcttcgcgc
     5641 agcgcatctt tcatatcgga cttgatgctg accttgactt caatcttacg aagatcgtcg
     5701 ctgaggctga tatcctgcac tgttccgact tcaacgccct gataacgaac aggcgtacgg
     5761 cccgggacaa taccatccgc cgacataaag tcgatggtga cggtattact tcggtcctga
     5821 taactgtccc aaatcagcca accggcaatc attagcgcga tgaaaggcag cagccagaaa
     5881 ggtgagatac ggcgtttatt tttaatctgc gcttcagtcg tcgaagcggg cgtttcctga
     5941 ctcatgtgca tcccaaagta agcggctgtc cagccattcc acagcaagaa tagtcaaaat
     6001 tacggctgcg ccaaaataaa acgcagccgg tcccatagta aaagcgagga tctgatcgcg
     6061 attaatcagc gacatggtta aagatatgac aaacaggtcg agcatcgacc agcgtccaat
     6121 ccaggtcacc atccgcagta acagaatgcg tgtgcgtaaa ccttgctggc atttaaaatg
     6181 aatgctcaac agtaaagtga acatgacgat cactttagta aacggtacca gaatactggc
     6241 gataaacacg attccggcga ccgcaatgtt gctacttgcc agcgacataa tcccggaaag
     6301 aatcgtatct tcctgccggc caccattcag ataaatgata gaaataggca ataggttggc
     6361 tggtaacaac aaaacgattg acgctaacag cgccgcccag catttttgca aactatgacg
     6421 acgacgcagg cgtagcggga tatggcaacg cgggcagcga ccacgctgat caggatagcc
     6481 ggtaaaatgg cacccaagac agacacgaag tttctcgtcc ctacgcgtag cggggcgctg
     6541 cggataatat cgctcccaca gctcctcaac attaagatgt gacaacgtca ccgtcgttaa
     6601 aatcaccaac gccacaaaag agaacaagcc gacacccgcc tggatatggg cataatcctg
     6661 tacctttata gaagcaacgc caatgccgac caggtagata tccagcatta cccactcttt
     6721 aagacgctca agcatcagca gtaccggacg taaattcatt cccagtcggt taccaaacca
     6781 taaatatgct atggaggaca ccagaatgag gggggcacca ataacgcaga aaaagaccat
     6841 cgcccccgtt atcgtatcgc cctgtttggt catttgccag atgccttgca tgacgtttgc
     6901 gtcgatacga atacctaaca gccagatatg caacagcggt tcgccccagg caaacggcat
     6961 cagcaacagc atggtgaaag ccattgccgc caggcgcgtt agcgaccagt cgcgcccgtc
     7021 gcgaattttt gcctgacagc gcggacaata ggcactctga tgagaattta tctcgggcag
     7081 gctaaacagc atgtcacatt gcgggcaacg ttggtaatca ccacgcggca gttcctcgcc
     7141 gattgacctc actgttatct tttttgtcgg cgtaatttgt ggtgtgttaa gagccatgac
     7201 gtgccaaatg taataagaat taacattaat cttaacccat gatgggaatg atcttgagca
     7261 ttaacgcatt taatgcttat tttaataggc tggcagttcc aggtaagaca aacaagtgat
     7321 tatataatga acaaaacaga attttacgcg gatttaaatc gcgactttaa cgcgctgatg
     7381 gcgggagaaa ccagttttct ggcaacgctt gcgaacacca gtgcgttgtt atacgagcgt
     7441 ctcactgacg taaactgggc aggtttttat ttgcttgagg acgatacact ggtactcgga
     7501 ccatttcagg gcaaaattgc ctgtgtccgg atacccgtcg ggcgcggggt gtgcggcact
     7561 gcggttgccc gcaatcaagt gcagcgtatc gaggatgttc acgcgtttga cgggcatatt
     7621 gcctgtgatg cggcgagtaa ttctgaaatt gttctgccgc tggtggtgaa aaatcagatt
     7681 attggtgttc ttgacatcga tagtaccgtc ttcggtcgct ttacagacga ggacgagcaa
     7741 ggcttacgtc agcttgtggc acagcttgaa aaagtgcttg caacgacgga ttacaaaaaa
     7801 ttctttgcga gcgtcgcagg ataatcaacg gataacgtag catttactgg tagcgtcatt
     7861 ataatgacgc ctgttcatgc ctgcgcttgt tggctacgtc cgttgtaatc aggaaatttc
     7921 atggaaaatc aacctaagtt gaatagcagt aaagaagtaa tcgcgtttct ggccgaacgt
     7981 tttccccact gtttcagtgc ggaaggtgaa gcgcgtccgc tgaaaatcgg tatttttcag
     8041 gatttggtcg atcgtgttgc cggggaaatg aacctgagca aaacgcaatt acgatccgct
     8101 ttacgtctct acacttcgag ctggcgttat ctttacggtg ttaaacccgg cgcaacgcgt
     8161 gtcgatcttg acggcaaccc atgcggtgag ctggacgagc aacatgtaga gcatgctcgc
     8221 aagcagcttg aagaagcgaa agcgcgtgtt caggcacagc gtgctgaaca gcaggcgaaa
     8281 aaacgcgaag ctgccgcagc tgctggtgag aaagaagacg caccgcgccg cgaacgcaag
     8341 ccacgtccga ccacgccacg ccgcaaagaa ggcgctgaac gtaaacctcg tgcgcaaaag
     8401 ccggtagaga aagcgccaaa aacagtaaaa gcacctcgcg aagaacagca caccccggtt
     8461 tctgacattt cagctctgac tgtcggacaa gctctgaagg tgaaagcggg tcaaaacgcg
     8521 atggatgcca ccgtattaga aatcaccaaa gacggcgtcc gcgtccagct gaattcgggt
     8581 atgtctttga ttgtgcgcgc agaacacctg gtgttctgaa acggaggccg ggccaggcat
     8641 gaacatgttt tttaggctta ccgcgttagc tggcctgctt gcaatagcag gccagacctt
     8701 cgctgtagaa gatatcacgc gtgctgatca aattccggta ttaaaggaag agacgcagca
     8761 tgcgacggta agtgagcgcg taacgtcgcg cttcacccgt tctcattatc gccagttcga
     8821 cctcgatcag gcattttcgg ccaaaatctt tgaccgctac ctgaatctgc tcgattacag
     8881 ccacaacgtg ctgctggcaa gcgatgttga acagttcgcg aaaaagaaaa ccgagttagg
     8941 cgatgaactg cgttcaggca aactcgacgt tttctacgat ctctacaatc tggcgcaaaa
     9001 gcgccgtttt gagcgttacc agtacgcttt gtcggtactg gaaaagccga tggatttcac
     9061 cggcaacgac acttataacc ttgaccgcag caaagcgccc tggccgaaaa acgaggctga
     9121 gttgaacgcg ctgtgggaca gtaaagtcaa attcgacgag ttaagcctga agctggcagg
     9181 aaaaacggat aaagaaattc gtgaaaccct gacacgccgc tacaaatttg ccattcgtcg
     9241 tctggcgcaa accaacagcg aagatgtttt ctcgctggca atgacggcgt ttgcgcgtga
     9301 aatcgacccg cataccaact atctttcccc gcgtaatacc gaacagttca acactgaaat
     9361 gagtttgtcg ctggaaggta ttggcgcagt gctgcaaatg gatgatgact acaccgttat
     9421 caattcgatg gtggcaggtg gtccggcagc gaagagtaaa gctatcagcg ttggtgacaa
     9481 aattgtcggt gttggtcaaa caggcaagcc gatggttgac gtgattggct ggcgtcttga
     9541 tgatgtggtt gccttaatta aagggccgaa gggcagtaaa gttcgtctgg aaattttacc
     9601 tgctggtaaa gggaccaaga cccgcactgt aacattgacc cgtgaacgta ttcgtctcga
     9661 agaccgcgcg gttaaaatgt cggtgaagac cgtcggtaaa gagaaagtcg gcgtgctgga
     9721 tattccgggc ttctatgtgg gtttgacaga cgatgtcaaa gttcaactgc agaaactgga
     9781 aaaacagaat gtcagcagtg tgatcatcga cctgcgtagc aatggcggtg gggcgttgac
     9841 tgaagcggta tcgctctcag gtctgtttat tcctgcgggt cccattgttc aggtccgcga
     9901 taacaacggc aaggttcgtg aagacagcga taccgacggg caggtgttct ataaaggccc
     9961 gctggtggta ctggttgacc gtttcagtgc ttcggcttca gaaatctttg ccgcggcaat
    10021 gcaggattac ggtcgtgcgc tggttgtggg tgaaccgacg tttggtaaag gcaccgttca
    10081 gcaataccgt tcattgaacc gtatttacga tcagatgtta cgtcctgaat ggccagcgct
    10141 gggttctgtg cagtacacga tccagaaatt ctatcgcgtt aacggcggca gtacgcaacg
    10201 taaaggcgta acgccagaca tcatcatgcc gacgggtaat gaagaaacgg aaacgggtga
    10261 gaaattcgaa gataacgcgc tgccgtggga tagcattgat gccgcgactt atgtgaaatc
    10321 aggcgattta acggcctttg ggccggagct gctgaaggaa cataatgcgc gtatcgcgaa
    10381 agatcctgag ttccagaaca tcatgaagga tatcgcgcgc ttcaacgcta tgaaggacaa
    10441 gcgcaatatc gtttctctga actacgctgt gcgtgagaaa gagaataatg aagatgatgc
    10501 gacgcgtctg gcgcgtttga acgaacgctt taaacgcgaa ggtaaaccgg agttgaagaa
    10561 gctggatgat ctaccgaaag attaccaaga gccggatcct tatctggatg agacggtgaa
    10621 tatcgcactc gatctggcga agcttgaaaa agccagaccc gcggaacaac ccgctcccgt
    10681 caagtaatat caatcaggca caagaaattg tgcctgattt tttaacagcg gcaagatgcc
    10741 gtaaatcaga tgctacaaaa tgtaaagttg tgtctttctg gtgacttacg cactatccag
    10801 acttgaaaat agtcgcgtaa cccatacgat gtgggtatcg catattgcgt tttgttaaac
    10861 tgaggtaaaa agaaaattat gatgcgaatc gcgctcttcc tgctaacgaa cctggccgta
    10921 atggtcgttt tcgggctggt actgagcctg acagggatac agtcgagcag cgttcagggg
    10981 ctgatgatca tggccttgct gttcggtttt ggtggttcct tcgtttcgct tctgatgtcc
    11041 aaatggatgg cattacgatc tgttggcggg gaagtgatcg agcaaccgcg taacgaaagg
    11101 gaacgttggc tggtcaatac tgtagcaacc caggctcgtc aggcagggat cgctatgccg
    11161 caagtggcta tctaccatgc gccggacatc aacgcttttg caaccggtgc gcgccgtgat
    11221 gcctctctgg ttgctgtcag caccggtttg ctgcagaaca tgagcccgga tgaagccgag
    11281 gcggtaattg ctcacgaaat cagccacatc gccaatggtg atatggtcac catgacgctg
    11341 attcagggcg tgatgaacac cttcgttatc tttatttccc gtattctggc gcagcttgcc
    11401 gcgggtttta tgggcggaaa tcgtgatgaa ggtgaagaga gcaacggcaa cccgctgatc
    11461 tactttgcgg ttgcaacggt tctggaactg gtgtttggta ttctggcgag cattatcacc
    11521 atgtggttct cgcgtcatcg tgaattccat gctgatgccg gttcggcaaa actggttggt
    11581 cgcgagaaaa tgattgccgc gctgcagcgc ctgaaaacca gctatgaacc gcaagaagca
    11641 accagcatga tggctttctg cattaatggt aagtcgaaat cgctcagtga gttgttcatg
    11701 actcacccgc cgctggataa acgaattgaa gctctgcgta cgggtgaata cctgaagtaa
    11761 tatgaagtaa atactaaaaa gtgcgtcctg atcg
//
LOCUS       DAZPEB010000127        11784 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZPEB010000127 DAZPEB010000000
VERSION     DAZPEB010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11784
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.128"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..1414
                     /locus_tag="ACHURJ_002927"
     CDS             <1..1414
                     /locus_tag="ACHURJ_002927"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFR1281799.1"
                     /translation="WGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVPREVTEGVID
                     GLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEPNEEGLQFYD
                     DLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTVFTRYKEKVK
                     YWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYELVASALAVQT
                     GKSINPEFNIGCMIAMYPIYPLTCVPNDMMMATKAMHRRYWFTDVHARGYYPQHMLNY
                     FARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVEPRDLVSNPY
                     IDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTVNDHYRIDYF
                     ASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKDNEGKGTLER
                     IRKASFYWYRDLIANNGENI"
     gene            complement(1471..1689)
                     /gene="marB"
                     /locus_tag="ACHURJ_002928"
     CDS             complement(1471..1689)
                     /gene="marB"
                     /locus_tag="ACHURJ_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFR1281800.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(1721..2104)
                     /gene="marA"
                     /locus_tag="ACHURJ_002929"
     CDS             complement(1721..2104)
                     /gene="marA"
                     /locus_tag="ACHURJ_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFR1281801.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            complement(2124..2558)
                     /gene="marR"
                     /locus_tag="ACHURJ_002930"
     CDS             complement(2124..2558)
                     /gene="marR"
                     /locus_tag="ACHURJ_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFR1281802.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            2770..3435
                     /gene="marC"
                     /locus_tag="ACHURJ_002931"
     CDS             2770..3435
                     /gene="marC"
                     /locus_tag="ACHURJ_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFR1281803.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            complement(3460..4650)
                     /gene="ydeA"
                     /locus_tag="ACHURJ_002932"
     CDS             complement(3460..4650)
                     /gene="ydeA"
                     /locus_tag="ACHURJ_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFR1281804.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(4863..5913)
                     /gene="yneK"
                     /locus_tag="ACHURJ_002933"
                     /pseudo
     CDS             complement(4863..5913)
                     /gene="yneK"
                     /locus_tag="ACHURJ_002933"
                     /inference="COORDINATES: protein motif:HMM:NF033404.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            complement(5991..6872)
                     /locus_tag="ACHURJ_002934"
     CDS             complement(5991..6872)
                     /locus_tag="ACHURJ_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR1281805.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAIVLRVPSNLTIRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSAKQGYQ"
     gene            6973..8362
                     /gene="sad"
                     /locus_tag="ACHURJ_002935"
                     /pseudo
     CDS             6973..8362
                     /gene="sad"
                     /locus_tag="ACHURJ_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
     gene            8426..9352
                     /gene="glsB"
                     /locus_tag="ACHURJ_002936"
     CDS             8426..9352
                     /gene="glsB"
                     /locus_tag="ACHURJ_002936"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFR1281806.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            9352..9711
                     /locus_tag="ACHURJ_002937"
     CDS             9352..9711
                     /locus_tag="ACHURJ_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFR1281807.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            9850..11268
                     /locus_tag="ACHURJ_002938"
     CDS             9850..11268
                     /locus_tag="ACHURJ_002938"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFR1281808.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIVAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVVMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            11495..11734
                     /locus_tag="ACHURJ_002939"
                     /pseudo
     CDS             11495..11734
                     /locus_tag="ACHURJ_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="catalyzes the formation of D-tagaturonate from
                     D-altronate; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
BASE COUNT         2987 a         2661 c         3031 g         3105 t
ORIGIN      
        1 atggggcggc gcggtagccg cgcatcagtt ggaaggtggc tggaatgaag gaggaaaagg
       61 catcagtatc gctgatgtga tgactgctgg cgctcacggg gtgccgcgtg aagtgacaga
      121 aggcgttatc gacgggctta attatcccaa tcatgaagca attgattttt atcatcgcta
      181 taaaacagat attcagttat ttgccgagat gggattcaaa tgctttcgaa cttccattgc
      241 ctggacacga atctttccgc aaggtgacga acaggagccg aatgaagagg gtttacaatt
      301 ttatgatgat ctgttcgatg aatgccttaa gcagggaatg gaacctgtgg tgacgctttc
      361 acattttgag atgccttatc atctggtgac aaaatatggt ggctggcgaa accgtaaact
      421 gatcgacttt tttatccgct tcgcatcaac ggtcttcacg cgctataaag aaaaagtaaa
      481 gtactggatg acgtttaacg aaatcaataa tcaggtgaat ttcagcgaaa gcctgtgtcc
      541 atttactaat tccggtatct tgtattcgcc agaggaagat atcaatgagc gcgaacaaat
      601 aatgtaccag gcggtacatt acgagttagt tgccagtgcc ctggcggtac agactggaaa
      661 atcgatcaat cctgaattta acattggctg tatgatcgcc atgtatccca tctatcctct
      721 gacgtgtgta cccaacgata tgatgatggc cacgaaagca atgcatcgtc gttactggtt
      781 tactgatgtt catgctcgtg gatattatcc gcaacatatg ctgaattact ttgccaggaa
      841 aggattcaac ctcgatatca caccagaaga taacgcgatt cttgccagtg gttgtgtcga
      901 ctttatcggc tttagctact acatgtcttt tacgacacaa ttttcgcccg ataacccgca
      961 actggattat gttgaaccac gagatttggt cagcaaccct tatatcgata catccgaatg
     1021 gggatggcaa attgatccgg cagggctacg ttattcactc aactggttct gggatcattt
     1081 ccagttgccg ctgtttattg tcgaaaatgg atttggtgcg gttgaccaga gacaagctga
     1141 cggcacggtg aacgatcact atcgcattga ttactttgct tcccatattc gggaaatgaa
     1201 aaaagccgta gttgaagatg gtgttgactt aattggctac accccgtggg gctgcattga
     1261 cctggtttct gccggaacag gggaaatgaa aaagcgctac ggaatgattt atgtcgataa
     1321 agacaacgaa gggaagggaa cgctggaacg gatacgtaaa gcgtcgtttt actggtatcg
     1381 ggatctcatc gccaacaatg gcgaaaatat ttgagacttg aggaagatat tacggagatg
     1441 tcaggctctg tgatcagagc ctgacaataa ctacatagcg tgttgattat aatagggcac
     1501 gccgagcgca tccgacttat cactgccagt acccatgtga tttaaatcaa acggtggttg
     1561 ttcctgcgat gggggaacaa ccacgacatt ggcacaagaa gtaacaactg gctgcgtggt
     1621 ttgttccgca acgccctgcg cggaaaagag aataagcgca gctgctattg cggatgaaag
     1681 tggtttcatg attgcctcag tgacgttgtc acgttatcaa ctagctattg taatgattta
     1741 atggatgtaa aaagcgcgat tcgccctgca tattggtcat ccggtattta tgtggcggaa
     1801 catcaaagta atttttgaag gttcgggtca gagtttgttg cgactcgaag ccatatcgtt
     1861 ctgccagata gagtatcggc tcgttacttt ccttcagctt ttgcgcgatt tccgtcatct
     1921 tacggctgcg gatgtattgg cctaatgaat gaccggtttc ttttttaaac atccgttgca
     1981 ggtgccattt agagtaacct gaacgctctg acactttctc cagtgacagt ggcgattcca
     2041 ggttgtcctc gatccagtcc aaaatgctat gaatggtaat agcgtcagta ttgcgtctgg
     2101 acatcgtcat acctcttttt tgtttacggc aggactttct taagcaaatg ctcaagtgtt
     2161 gccacttcgt ccgccgtcag gttttttgtt aattcttgat gcaggtcctg gccaactaat
     2221 tgatggcatt gttcgcatat tgccgcgccg ctggtggtaa gttttaccag tacgccgcgc
     2281 ttatcattcg ggttcggcaa cctttcaacc cagcctttac agaccaggcg atccagcata
     2341 cgggtcagtg ctccaaggtc gacagacaac acttttttca gttcaaccgg agtaatacac
     2401 gccgcgcagc ggatagagca gagcacctta aactgtgccg cggtaatatc cagcggagac
     2461 agatactcgt taagcagacg atctttcttc tgattaacca tatggattaa gcgacccaat
     2521 ggaataattt cattgaacag atcgctggta cttttcacat tagttgccct ggcaagtaat
     2581 tagttgcagg gggtaatatt gcccaggcaa gtataagtca aataaatgaa ttgaccgatg
     2641 ccacgttttg ctaaatcggt tcaagttttg gtcaatgcga tgcaaggtta gctaaagagt
     2701 gacactttat ccttgtttac cccttatact tttcgctgat aacccagata cactggataa
     2761 caaccaccaa tgttagattt gtttaaagca attggcctgg ggctggtggt gttactgccg
     2821 ttagctaacc cattaacaac tgtcgcgtta tttcttggcc tggcaggcaa catgaacagt
     2881 gccgaacgta atcgtcagtc gttgatggcc tcggtgtacg tatttgccat catgatggtg
     2941 gcgtattacg ccgggcaact ggtgatggat acatttggca tttcgattcc cggtctgcga
     3001 attgcaggcg gcttaatcgt cgcgtttatc ggttttcgga tgctctttcc gcaacagaaa
     3061 gcgattgatt caccggaggc gaaaagcaag tcggaagagc tggaagatga acccagtgcc
     3121 aatattgcct ttgtgccgct agcaatgcca agtaccgctg gtccgggaac catagcgatg
     3181 attattagct cggcgtccac agtgcgtcag agttcaacct tcgctgattg ggtactgatg
     3241 gttgcgccgc cgctgatctt tttcctggtg gcggttattt tgtggggaag cctacgcagc
     3301 tccggcgcaa ttatgcggct ggtgggcaag ggtggcattg aagcgatctc ccgtctgatg
     3361 ggattcctgc tggtatgtat gggtgtgcag tttattatta acggcatcct ggaaatcatt
     3421 aaaacgtacc attaaaaaag gcccgaatgg gcctttcaac tattgcgtct gttcttcgag
     3481 tgtcactggc cagcggcgaa atataatgat tgaccatatt aacgcggcaa aagcaggcac
     3541 cgcgcccaca taaccaatca tcgacattga ccagtgcaaa ctcacctgat tacctaccaa
     3601 cgcacccgcc ccgattccaa tattaaatat gccggagaat agcgccatcg cgacgtcggt
     3661 agcatctggt gccagcgcca gcactttaac ctgcatacca agcccgatga tcatcatcgc
     3721 gatcccccag aaaatactca gcaccccgag gtgtatttca ctgttcgccg caggtagcag
     3781 cagtgccaga cacaccagca acagcgcaat cgccgtactc accaacgcag acgcatactg
     3841 attacccagt ttaccgaaaa tcacgctgcc aataatgccc gcaccaccga gtaataacag
     3901 taatgccgtg gcaaagttgg cgctgaatcc cgcaatgttt tgcacaaaag gctcgatata
     3961 gctgtatgcc gtgtaatggg cggtgacaac caccacagtt aacaaataaa tgctcatcaa
     4021 tgccgggcgg cggaacaata gcgggaggct tttcaatgaa ccggaatgct cactgggcag
     4081 taagggaagt aacttaatca ggcacaaaag ggtgataagc gccccaatac caatcgcgaa
     4141 gaaggtcatt cgccaaccga aatactgccc cacaatgcgc ccgagaggta aacctaagac
     4201 catcgccagt gctgtaccgg tggcaattaa actcaaggcc tgtgcccgct tcccggccgg
     4261 agccatacgg atcgccagag acgccgtaat cgaccagaaa atcgcatgtg caaaagccac
     4321 accaatgcga ctgatcacca gaacggtaaa gctccacgat aaaaacgaca gtacgtggct
     4381 ggcaataaac accacaaaca ggcagatcag taatttgcgc cgttcaacct gactggtcat
     4441 taacataaaa ggcaatgaca ttagcgctac tacccatgcg taaatggtca acatgatgcc
     4501 gacctgagcg gtttgcatgt gaaaactttg cgcaatgtca gagagcaggc caacagggac
     4561 aaattcggtg gtgttgaaga tgaaggcggc gactgccagc gtaacgaccc gtagccacgc
     4621 cactttgcgg gaaacagtgt ttgttgtcat aacagattat cgggtgagtt gcgaaaaagt
     4681 gatgaaatga tcttaaaacg gttaatgatg tgaatacaat gcatttgtga tctggatcgc
     4741 gttttccgtt tctctgacgc tgattctgtt ccgcgtcaga gagtcggtaa catcacggtt
     4801 taatgacgtt cagggaggtc tggcgttttg gcggttgcgg gtataggtgg tctgacaggg
     4861 gagcgaggcc tgtttttcac caggtcttcg tgagtaactt ttggcggcgt ttcatttttt
     4921 acaaacagtt tgagggtcct gcgcgccata tgcttgataa ttttatcatc acttaatgat
     4981 gctggcatca acacgtcatt cagatcactt tcattgggat tcataaattg gtgatagtgc
     5041 aataacatat tggcgaaaaa taaaaaaagt cctgaaaatg aggagattct tgttctgcta
     5101 aaagggataa aaagcagatg cagacttcgg gtagtacatg tttgttagaa cctaatgaat
     5161 ccatcaggct ttgcaataaa tcctctttta attcgccgtc tgttatgtcg agaatggcgg
     5221 tgatcaagtt aggatcttta ttgacgaacc atctgtgaac ctcagagtta ctccataatc
     5281 gggggagtat gacaaaatta ccgcgagaga taaaattcag gacaagcaaa tcaaactggc
     5341 gtaagtaatc tgcagcggta tggtgatccg gaggcagggg ttcaatatgt ggtgtatgcg
     5401 cttttttgat ggggacgcca aggtggatca ttggaaattg ttcaataaaa tcattcggaa
     5461 acagaaagag cacacgctcg acgccggtgt gaaatacgcg ccccatagtg acaggctttg
     5521 ggttgggagc gcaaaaatat taatttctcc atcatggcga aaattatcta aataaatagg
     5581 gtttaaattt attactacct gaaattcaat attttctttg tgtcttccat tcttgacggg
     5641 ataagtgagt ttaaactgaa ccttttcatt gtatagtgtg tcgttacccc gaaaagaagt
     5701 cctctgaata tatgataagt tatcaaggtt gttaaatatc ttttttgtgg ctgagttggt
     5761 gtttaaagac gtgcgccact cttcatcaca tattatggta ctgagtgcat caataaaatt
     5821 ctttaaaact tcttttacct gaggtgcaat attacgtacg ggaaaatcat caggaagaaa
     5881 tatgtaatta ctgatgctta ctggcgtaac catatttgcc ccatatagtc ataagtcatc
     5941 ggaacatcac tatatagggc atttgagctg caagaactat caaaaatagc tcattggtaa
     6001 ccctgttttg ccgagtcagg cacatccagt agttgaataa atgcctcaag cggacgcgtt
     6061 ttcgcaccac gacgccagac cagccaggtt gttaaccaac gccattgctc agctaacggc
     6121 cacgcttcaa cctggtgatg ccccggcata ctttccagca tagagcgtgg aataagcgca
     6181 atgcctgctc ctgcgaccac acaggccaac atcccgtgat acgactccat ctcgtgaata
     6241 gttcccggag cggcaccgtc agcatgaaac cagctctcga agtggcgacg atacgaacaa
     6301 ttggcgcgga aggcataaat gttactgccg ttaacctgac tggcgcggat tactggcgca
     6361 tgaccttgtg gcgtgacgat catcagctcc tcgcggtata ccggtatccc gtcaatggca
     6421 gtatggttaa tgggcccatc aataaatgcc gcattcagtt ttccctccag cactccttcc
     6481 agcatagtgc cggaagggcc ggtggaaagt gaaaactgaa ttttgggata acgatggttg
     6541 aattccgcca gcgtggccgg aatgcgcact gctgcggtgc tttccagcga accaagagaa
     6601 aataaacctt gcggctcatc gccagcgaca acgctccgcg cttcatccac taacgcgaga
     6661 atttgttggc tatagcgtaa aaagttatgc ccggcaggag agagacgtaa acgctgattc
     6721 tcgcgaataa acagatctac ccccagttct gtttccagct ggcgtagacg gatagtgaga
     6781 ttcgacggca cgcgaagcac tattgctgct gcctgggtta tgctgccagc ttcggcaacc
     6841 gcgttgaaca tctccagttg ggtcagatcc ataatattct cgattcgtga ataagtggct
     6901 taatattatt cattttaaag caagagtaaa tctgcgtatc ttcataccat gactcataaa
     6961 ggagataccc cgatgaccat tactccggca actcatgcaa tttcgataaa tcctgccacg
     7021 ggtgaacaac tttctgtgct gccgtgggct ggcgctaacg atatcgaaaa cgcacttcag
     7081 ctggcggcag caggctttcg cgactggcgc gagacaaata tagattatcg tgctgaaaaa
     7141 ctgcgtggta tcggtaaggc tctgcgcgcc cgtagcgaag aaatggcgca aatgatcacc
     7201 cgtgaaatgg gcaaaccaat caatcaggcg cgcgctgaag tggcgaaatc ggcgaatttg
     7261 tgtgactggt atgcagaaca tggtccggca atgctgaagg cggaacctac gctggtggaa
     7321 aatcagcagg cagttattga gtatcgaccg ttggggatga ttctggcgat tatgccgtgg
     7381 aactttccgt tatggcaggt gatgcgtggc gcggttccca tcattcttgc aggtaacggc
     7441 tacttactta aacatgcgcc gaatgtgatg ggctgtgctc agctcattgc ccaggtgttt
     7501 aaagatgcgg gaatcccgca aggcgtatat ggctggctga atgccgacaa cgacggtgtc
     7561 agtcaaatga ttaaagattc gcgcattgct gctgtcacgg tgaccggaag tgttcgtgcg
     7621 ggagcggcta ttggcgcaca ggctggagcg gcactgaaaa aatgcgtact ggaactgggc
     7681 ggttcggatc catttattgt gcttaacgat gccgatctgg aactggcggt taaagcggcg
     7741 gtagccggac gttatcagaa taccggacag gtttgtgcag cggcaaaacg ctttattatc
     7801 gaagagggaa ttgcttctgc atttaccgaa cgttttgtgg cagctgcggc agccattgaa
     7861 aatgggcgat ccccgtgatg aagagaacgc tctcggacca atggctcgtt ttgatttacg
     7921 tgatgagctg catcatcagg tggagaaaac cctggcgcag ggtgcgcgtt tgttactggg
     7981 cggggaaaag atggctgggg caggtaacta ctatccgcca acggttctgg cgaatgttac
     8041 cccagaaatg accgcgtttc gggaagaaat gtttggccct gttgcggcaa tcaccgttgc
     8101 gaaagatgca gaacatgcgc tggaactggc taatgatagt gagttcggcc tttcagcgac
     8161 catttttacc accgacgaaa cacaggccag acagatggcg gcacgtctgg aatgcggtgg
     8221 ggtgtttatc aatggttatt gtgccagcga cgcgcgagtg gcctttggtg gcgtgaaaaa
     8281 gagtggcttt ggtcgtgagc tttcccattt cggcttacac gaattctgta atatccagac
     8341 ggtgtggaaa gaccggatct gacgcgacat attcagctct gatatactcg caggtctttt
     8401 cagacctgcg gtccaggagt agaaagtggc agtcgccatg gataatgcaa ttttagaaaa
     8461 catcttgcgg caagtgcggc cgctcattgg tcagggtaaa gtcgcggatt atattccggc
     8521 gctggctaca gtagacggtt cccgattggg cattgctatc tgtaccgttg acggacagct
     8581 ttttcaggcc ggagacgcgc aagaacgttt ttccattcag tctatttcca aagtgctgag
     8641 tctcgttgtc gccatgcgtc attactccga agaggaaatc tggcaacgcg tcggcaaaga
     8701 tccgtctgga tcaccgttca attctttagt gcaactggaa atggagcagg gtattccgcg
     8761 taatccgttt attaatgctg gtgcgctggt ggtctgcgat atgttgcaag ggcgattaag
     8821 cgcaccacgg caacgtatgc tggaagtcgt gcgcggctta agcggtgtgt ctgatatttc
     8881 atacgatacg gtggtagcgc gttccgaatt tgaacattcc gcgcgaaatg cggctatcgc
     8941 ctggctgatg aagtcgtttg gcaatttcca tcatgacgtg acaaccgttc tgcaaaacta
     9001 ctttcattac tgcgctctga aaatgagctg tgtagagctg gcccggacgt ttgtctttct
     9061 ggctaatcag gggaaagcta ttcatattga tgaacctgtg gtgacgccaa tgcaggcgcg
     9121 gcaaattaac gcgctgatgg cgaccagtgg tatgtaccag aacgcggggg agtttgcctg
     9181 gcgggtgggg ctaccggcga aatctggcgt tggtggcggt attgtggcga ttgttccgca
     9241 tgaaatggcc atcgctgtct ggagtccgga actggatgat gcaggtaact cgcttgcggg
     9301 tatcgccgtc cttgaacaat tgacgaaaca gttagggcgt tcggtttatt aatgcagtct
     9361 ctcgatccac tcttcgcgcg tttatcccgt tcaaaatttc gctctcgctt tcgtctgggc
     9421 atgaaagagc gtcagtattg cctggagaaa ggcgcgccag tcatcgaaca acatgcggcg
     9481 gattttgtcg ctaaacgtct tgctccggca ttaccggcta atgacggtaa gcaaacaccc
     9541 atgcgcgggc atccggtgtt tatcgctcag cacgccaccg cgacctgctg tcgcggttgt
     9601 ctggctaagt ggcataacat tccgcagggt gtttcgttaa gcgaagaaca acaacgttat
     9661 atcgtggcgg taatctatca ctggctggtt gtacagatga atcagccatg agaattgcgg
     9721 aaaacgataa aatagcattc ccagggtacc tttatttgcc gccgctgtta gcgaacatgc
     9781 tatcctttat gagaatttac ttattacgtt gagataattg ccacccgcaa ttgtatggac
     9841 agataattaa tgcatgtaca ccccatttca actttccgtt tgttccagga aggtcatctg
     9901 ctacgtaata gcatcgctat ttttgcgcta accacgctgt tctattttat tgttgcagag
     9961 ttacggctgg ttcacgaact ttctcttttc tggccgctga atggcgtaat ggcgggggtg
    10021 tttgcccgct atgtctggct taatcgactg cattactatg cgatcagtta tgtggtgatg
    10081 ctggtttatg atgccataac caccgaatgg ggactggttt cactggttat caatttctcc
    10141 aatatgatgt ttattgttac cgtcgcctta ctggtcgtgc gggataagcg tctggggaaa
    10201 aataagtatg agccagtaag tgccttacgg ctatttaatt actgtctgat tgccgcatta
    10261 ttatgcgcta ttgtcggggc gattggttcg gtcagtattg atagtctgga tttctggcct
    10321 ttgcttgcgg actggttcag tgagcaattc tcaacgggcg tgttgatcgt gccttgtatg
    10381 ctgacgttgg ctattcctgg agtgctgccg cgctttaaag cagaacagat gatgcccgcc
    10441 atcgcgctta ttgtgtcggt tattgcctcg gtagtcattg gcggagcggg gagtctggcg
    10501 ttcccgctgc ctgcattaat ctggtgtgca gtgcgctata cgccgcaggt aacatgtctg
    10561 ttgacctttg tcaccggtgc ggtggaaatc gtactggtgg caaattcggt gattgatatc
    10621 tcggtcggtt cgccgttctc cattccggaa atgttctccg cacgtctcgg tattgccacg
    10681 atggcgatat gcccaattat ggtttctttt agcgtggcag cgatcaattc gctaatgaag
    10741 caagttgcgc tgcgagccga ctttgatttt ctgactcagg tttactcacg gtccggtctt
    10801 tatgaggcgc tgaaaagtcc atcgctgaaa cagacacaac atctgacggt catgctgctt
    10861 gatatcgact atttcaaaag cattaacgat aactatggac atgaatgtgg cgataaagtg
    10921 ttaagcgtgt ttgcccggca tattcagaag attgtcggtg ataaggggct ggtggcgcgc
    10981 atgggcggcg aagaatttgc tgttgcagtg ccgtcggtga atcctgtcga tggtctgcta
    11041 atggcggaaa aaatccgtaa aggcgttgaa ctgcaaccat tcacctggca acaaaaaacg
    11101 ctttatctca cggtaagtat tggcgtcggt agtggtcgcg catcgtaccg aacgctgacc
    11161 gatgatttta ataaattgat ggtcgaagcc gatacgtgtc tgtatcgctc gaagaaagat
    11221 gggcgcaacc gtaccagcac tatgcgttac ggtgaggaag ttgtctgaca tctttattca
    11281 ttccgcgcga tttgaatgca atgtgttcta ccactttatg atccaggaaa aggcgtattt
    11341 cctcgattaa accatgatcc gcgccacact tttctcataa aactgctcat taatcatcag
    11401 gagttgatca aaacaggggt tcggcgtatt aaattttgct aacctgtcgt accaattcaa
    11461 gcgggttgta tgactaatca gaagggaacc cattgtgaaa acactaaatc gtcgcgatat
    11521 tcccggtgca cagtatccag aacgtatcat tcagtttggt gaaggtaact tcctgcgcgc
    11581 ctttgttgac tggcaaatcg atctcctgaa tgagcacacc gatctgaatt ctggcgtggt
    11641 cgttgttcgt ccgattgaaa cttcattccc gccgtcactg agcacgcagg atggtctgta
    11701 caccaccatt atccgtggcc tgaatgagaa ggggtgaagt ggtcaacaaa aactggccac
    11761 cgagttagag tttttccagt atcg
//
LOCUS       DAZPEB010000128        11776 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZPEB010000128 DAZPEB010000000
VERSION     DAZPEB010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11776
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.129"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(12..662)
                     /gene="hxpA"
                     /locus_tag="ACHURJ_002940"
     CDS             complement(12..662)
                     /gene="hxpA"
                     /locus_tag="ACHURJ_002940"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFR1281809.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTCLEHIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     AEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQLNGDVIIQ"
     gene            complement(673..1167)
                     /locus_tag="ACHURJ_002941"
     CDS             complement(673..1167)
                     /locus_tag="ACHURJ_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFR1281810.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACLRQYHLSANEINQ
                     IINA"
     gene            complement(1251..1706)
                     /gene="yfbV"
                     /locus_tag="ACHURJ_002942"
     CDS             complement(1251..1706)
                     /gene="yfbV"
                     /locus_tag="ACHURJ_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFR1281811.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            2044..3246
                     /gene="ackA"
                     /locus_tag="ACHURJ_002943"
     CDS             2044..3246
                     /gene="ackA"
                     /locus_tag="ACHURJ_002943"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFR1281812.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            3321..5465
                     /gene="pta"
                     /locus_tag="ACHURJ_002944"
     CDS             3321..5465
                     /gene="pta"
                     /locus_tag="ACHURJ_002944"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFR1281813.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNQEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            5655..7175
                     /gene="yfcC"
                     /locus_tag="ACHURJ_002945"
     CDS             5655..7175
                     /gene="yfcC"
                     /locus_tag="ACHURJ_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFR1281814.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVEPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATMIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQITVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(7208..7750)
                     /gene="yfcD"
                     /locus_tag="ACHURJ_002946"
     CDS             complement(7208..7750)
                     /gene="yfcD"
                     /locus_tag="ACHURJ_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFR1281815.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(7808..8359)
                     /gene="yfcE"
                     /locus_tag="ACHURJ_002947"
     CDS             complement(7808..8359)
                     /gene="yfcE"
                     /locus_tag="ACHURJ_002947"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFR1281816.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(8415..9047)
                     /gene="yfcF"
                     /locus_tag="ACHURJ_002948"
     CDS             complement(8415..9047)
                     /gene="yfcF"
                     /locus_tag="ACHURJ_002948"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFR1281817.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASVAMAEHLLALGQPNLFGEW
                     CIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            9183..9830
                     /gene="yfcG"
                     /locus_tag="ACHURJ_002949"
     CDS             9183..9830
                     /gene="yfcG"
                     /locus_tag="ACHURJ_002949"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFR1281818.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGELGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLTMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            9887..10249
                     /gene="folX"
                     /locus_tag="ACHURJ_002950"
     CDS             9887..10249
                     /gene="folX"
                     /locus_tag="ACHURJ_002950"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFR1281819.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            10270..11163
                     /locus_tag="ACHURJ_002951"
     CDS             10270..11163
                     /locus_tag="ACHURJ_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFR1281820.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIDSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(11211..>11776)
                     /gene="rpnB"
                     /locus_tag="ACHURJ_002952"
     CDS             complement(11211..>11776)
                     /gene="rpnB"
                     /locus_tag="ACHURJ_002952"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFR1281821.1"
                     /translation="GYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSA
                     FPLVDITVVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKA
                     LFNYVLQTGDAQRFRAFIGEIAERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQE
                     MLDRGLDRELVMMVTRLSPDDLIAQSH"
BASE COUNT         2819 a         3121 c         2946 g         2890 t
ORIGIN      
        1 gatcaggtgt ttcactgaat aataacatcg ccatttagct gtttggtcac agtaatttgc
       61 tccagactgt ggaggaccaa atcgacctca ttcaggcgcg gggtatctgc cggagcgtta
      121 accgcaatga catggcaacc cgccgccagg ccagaaagca cgccagcggg agcatcttcc
      181 gccaccacac actcctgcgg cgcaagcccc agcagctgcg cacctaacag atacgcatca
      241 ggttctggtt ttccgcgctt cactcgctca gcggttacaa acacctctgg tgcgggaagc
      301 ccagctattt tatggcgcgc tcgcgctacc ggcatggagc cagaagtcac aatggcccac
      361 ggaatacctg ctttattcaa atgattgagt aaggcgattg cccccggaag cgcggtaata
      421 ccttcggttt ccgtggcctc gatgtgctcc agacacgtaa actcggcggc aatatcagcc
      481 tcggatttgc ccgccataaa atggcgcaaa gaggtgatcg cctgtttacc gtgaatgaaa
      541 gccagcacct cttccggcgc taacccatga cgtctggccc agttgctcca cgcccgttct
      601 accgcaggca gggaatccac cagcgttcca tcaagatcaa acagaaaacc tttgcaccgc
      661 acgcgggcct cctcaggcgt tgataatttg attaatttcg ttggcgctca aatggtactg
      721 acgcaggcag gcatgccaca cattaagcat gcgctgatat ttttcccaca ttggcgtctg
      781 ggcgttaaaa ccatgagtcc cagcgtcaaa atgggtatag cgcccttcca cattaaccat
      841 aaagcggaca taaccgaggt aacgtgcttc agtggcggcg tcaaagccga ggaaggtgac
      901 acgacgttca tcgatggatt gctggtcctg caaattagac caggaaacat gcaacgcatg
      961 atacatctcc ataatgtcga tgatagtgcg gcaggtttct tctttcagct cgccaaactc
     1021 gcgatccaat tcacgcatct gtaatccgta accacgctca ataattgttt gcaggcgacg
     1081 gtaacgttca gcatttgccg gatcgagcat agtcatcatc ttgtactgat tagacaaaat
     1141 aagacgttgc gcgttggtca tttccattgt tgactcctgt atcactctac tacggtgaaa
     1201 aaaaaagaag gctgagtatg ccttctttta tatgcgtaat caggggtcaa ttacaaatca
     1261 tcaaggaaag ttttatccag ttgtttgaag gcgcgcttaa gcgtgtcagc taatgcctgg
     1321 taatcaggct tgccttcaac gggtgccaac acctgtccag actcctgcaa tttaccgcga
     1381 acttcataaa accagttgag gatagcaggg ggtaatggcg ttacagaacg cttgcccagc
     1441 caccataatc cctgcatggg taaacttaag gcgaacagcg cagtggcaac tgccggccca
     1501 agctgaccgc ccagggcaat ctgccagcag agagtaaata cggcgatcgg cggcataaaa
     1561 cggatcgcat aacgcgtcat cttgataacg cgattttcga caaagaccgg ggcaaggcgt
     1621 ttttccagcg gccacgtctt tgagtaatgc tgtccccggc gaaacaagct aaaaaaatta
     1681 acagaacgat tatccggcgt tgacatgctt cacctcaact tcacatataa agattcaaaa
     1741 atttgtgcaa attcacaact cagcgggaca acattcaaaa cattttgtct tccataccca
     1801 ctatcaggta tcctttagca gcctgaaggc ctaagtagta catattcatt gagtcgtcaa
     1861 attcatatac attatgccat tggctgaaaa ttacgcaaaa tggcatagac tcaagatatt
     1921 tcttccatca tgcaaaaaaa atttgcagtg catgatgtta atcataaatg tcggtgtcat
     1981 catgcgctac gctctatggc tccctgacgt ttttttagcc acgtatcaat tataggtact
     2041 tccatgtcga gtaagttagt actggttctg aactgcggta gttcttcact gaaatttgcc
     2101 atcatcgatg cagtaaatgg tgaagagtac ctttctggtt tagccgaatg tttccacctg
     2161 cccgaagcac gtatcaaatg gaaaatggac ggcaataaac aggaagcggc tttaggtgca
     2221 ggcgccgctc acagcgaagc gctcaacttt atcgttaata ctattctggc acaaaaacca
     2281 gaactgtctg cgcagctgac tgctatcggt caccgtatcg tacacggcgg cgaaaagtat
     2341 accagctccg tagtgatcga tgagtctgtt attcagggta tcaaagatgc agcttctttt
     2401 gcaccgctac acaacccggc tcacctgatc ggtatcgaag aagctctgaa atctttccca
     2461 cagctgaaag acaaaaacgt tgctgtattt gacaccgcgt tccaccagac tatgccggaa
     2521 gagtcttacc tctacgccct gccttacaac ctgtacaaag agcacggcat ccgtcgttac
     2581 ggcgcgcacg gcaccagcca cttctatgta acccaggaag cggcaaaaat gctgaacaaa
     2641 ccggtagaag aactgaacat catcacctgc cacctgggca acggtggttc cgtttctgct
     2701 atccgcaacg gtaaatgcgt tgacacctct atgggcctga ccccgctgga aggtctggtc
     2761 atgggtaccc gttctggtga tatcgatccg gcgatcatct tccacctgca cgacaccctg
     2821 ggcatgagcg ttgacgcaat caacaaactg ctgaccaaag agtctggcct gctgggtctg
     2881 accgaagtga ccagcgactg ccgctatgtt gaagacaact acgcgacgaa agaagacgcg
     2941 aagcgcgcaa tggacgttta ctgccaccgc ctggcgaaat acatcggtgc ctacactgcg
     3001 ctgatggatg gtcgtctgga cgctgttgta ttcactggtg gtatcggtga aaatgccgcg
     3061 atggttcgtg aactgtctct gggcaaactg ggcgtgctgg gctttgaagt tgatcatgaa
     3121 cgcaacctgg ctgcacgttt cggcaaatct ggtttcatca acaaagaagg tacccgtcct
     3181 gcggtggtta tcccaaccaa cgaagaactg gttatcgcgc aagacgcgag ccgcctgact
     3241 gcctgatttc acaccgccag ctcagctggc ggtgctgttt tgtaacccgc caaatcggcg
     3301 gtaacgaaag aggataaacc gtgtcccgta ttattatgct gatccctacc ggaaccagcg
     3361 tcggtctgac cagcgtcagc cttggcgtga tccgtgcaat ggaacgcaaa ggcgttcgtc
     3421 tgagcgtttt caaacctatc gctcagccgc gtaccggtgg cgatgcgccc gatcagacta
     3481 cgactatcgt gcgtgcgaac tcttccacca cgacggccgc tgaaccgctg aaaatgagct
     3541 acgttgaagg tctgctttcc agcaatcaga aagatgtgct gatggaagag atcgtcgcaa
     3601 actaccacgc taacaccaaa gacgctgaag tcgttctggt tgaaggtctg gtcccgacac
     3661 gtaagcacca gtttgcccag tctctgaact acgaaatcgc taaaacgctg aatgcggaaa
     3721 tcgtcttcgt tatgtctcag ggcactgaca ccccggaaca gctgaaagag cgtatcgaac
     3781 tgacccgcaa cagcttcggc ggtgccaaaa acaccaacat caccggcgtt atcgttaaca
     3841 aactgaacgc accggttgat gaacagggtc gtactcgccc ggatctgtcc gagattttcg
     3901 acgactcttc caaagctaaa gtaaacaatg ttgatccggc gaagctgcaa gaatccagcc
     3961 cgctgccggt tctcggcgct gtgccgtgga gctttgacct gatcgcgact cgtgcgatcg
     4021 atatggctcg ccacctgaat gcgaccatca tcaacgaagg cgacatcaat actcgccgcg
     4081 ttaaatccgt cactttctgc gcacgcagca ttccgcacat gctggagcac ttccgtgccg
     4141 gttctctgct ggtgacttcc gcagaccgtc ctgacgtgct ggtcgccgct tgcctggcag
     4201 ccatgaacgg cgtagaaatc ggtgccctgc tgctgactgg cggctacgaa atggacgcgc
     4261 gcatttctaa actgtgcgaa cgtgctttcg ctaccggcct gccggtattt atggtgaaca
     4321 ccaacacctg gcagacctct ctgagcctgc agagcttcaa ccaggaagtt ccggttgacg
     4381 atcacgaacg tatcgagaaa gttcaggaat acgttgctaa ctacatcaac gctgactgga
     4441 tcgaatctct gactgccact tctgagcgca gccgtcgtct gtctccgcct gcgttccgtt
     4501 atcagctgac tgaacttgcg cgcaaagcgg gcaaacgtat cgtactgccg gaaggtgacg
     4561 aaccgcgtac cgttaaagca gccgctatct gtgctgaacg tggtatcgca acttgcgtac
     4621 tgctgggtaa tccggcagag atcaaccgtg ttgcagcgtc tcagggtgta gaactgggtg
     4681 cagggattga aatcgttgat ccagaagtgg ttcgcgaaag ctatgttggt cgtctggtcg
     4741 aactgcgtaa gaacaaaggc atgaccgaaa ccgttgcccg cgaacagctg gaagacaacg
     4801 tggtgctcgg tacgctgatg ctggaacagg atgaagttga tggtctggtt tccggtgctg
     4861 ttcacactac cgcaaacacc atccgtccgc cgctgcagct gatcaaaact gcaccgggca
     4921 gctccctggt atcttccgtg ttcttcatgc tgctgccgga acaggtttac gtttacggtg
     4981 actgtgcgat caacccggat ccgaccgctg aacagctggc agaaatcgcg attcagtccg
     5041 ctgattccgc tgcggccttc ggtatcgaac cgcgcgttgc tatgctctcc tactccaccg
     5101 gtacttctgg tgcaggtagc gacgtagaaa aagttcgcga agcaactcgt ctggcgcagg
     5161 aaaaacgtcc tgacctgatg atcgacggtc cgctgcagta cgacgctgcg gtaatggctg
     5221 acgttgcgaa atccaaagcg ccgaactctc cggttgcagg tcgcgctacc gtgtttatct
     5281 tcccggatct gaacaccggt aacaccacct acaaagcggt acagcgttct gccgacctga
     5341 tctccatcgg gccgatgctg cagggtatgc gcaagccggt taacgacctg tcccgtggcg
     5401 cactggttga cgatatcgtc tacaccatcg cgctgactgc gattcagtct gcacagcagc
     5461 agtaatctcg tcatcatccg cagctttgcg ctgcggatat ctgaaccgga aataatcact
     5521 atttccggtt ttttattctc ttaatctgca ttaatccttt ctgattatct tgcttaactg
     5581 cgctgcatca atgaattgcg ccatttcact ttgcatactt accactttgt tttgtgcaag
     5641 ggaatatttg cgctatgtcc gcaatcactg aatccaaacc aacaagaaga tgggcaatgc
     5701 ccgatacgtt ggtgattatc ttttttgttg ctattttaac cagccttgcc acctgggtag
     5761 ttccggtggg gatgtttgac agtcaggaag tgcagtatca ggttgatggt caaacaaaaa
     5821 cacgcaaagt cgtagagcca cactcatttc gcattctgac taacgaagca ggcgaacctg
     5881 agtatcaccg cgtacagctg ttcacgacgg gcgatgaacg cccgggcctg atgaacttcc
     5941 cgtttgaagg attaacctca ggatcgaaat acgggacagc tgttggcatc atcatgttta
     6001 tgctggtgat tggcggcgcg tttggcattg tgatgcgtac aggaaccatt gataacggta
     6061 tcctggcgct tattcgccat acccgcggga atgaaattct ctttattcct gcgctgttta
     6121 ttctgttttc acttggcggc gcggtatttg gtatgggaga agaggccgtc gcctttgcca
     6181 ttatcatcgc accgctaatg gtccggctgg gctatgacag tattaccacc gtcctggtga
     6241 cctatattgc cacgcaaatc ggttttgcca gttcgtggat gaacccgttt tgtgtggtcg
     6301 ttgctcaggg gattgccggc gttccggtgc tttctggctc cgggttgcgc atcgtggtgt
     6361 gggttatcgc cactatgatt ggcctgatct ttaccatggt gtacgcctca cgagtgaaaa
     6421 agaatcctct tctgtcacgc gtgcatgagt ccgaccgctt ctttcgtgaa aagcaggcgg
     6481 atgttgaaca acgtccgttt acctttggtg actggctggt attgattgtc ctgaccgccg
     6541 taatggtctg ggtgatttgg ggcgtgatcg ttaatgcctg gtttattcca gaaattgcca
     6601 gccagttctt caccatgggt ctggtgattg gcatcatcgg cgttgttttc cgccttaacg
     6661 gcatgacggt taataccatg gcttcatcct ttaccgaagg ggcgcgaatg atgatcgccc
     6721 ctgccctgct ggtgggtttc gccaaaggga ttttgctgct ggtcggtaat ggtgaagcgg
     6781 gtgatgccag cgtgttaaat accatcctca acagcattgc caatgccatt agcggtctgg
     6841 acaacgcggt cgcggcctgg tttatgttgc tcttccaggc ggtatttaat ttcttcgtga
     6901 cgtccggttc tggtcaggcg gcgttaacca tgccgttact ggcaccgctt ggcgatctgg
     6961 tcggtgttaa ccgtcagatt accgtgctgg cattccagtt tggtgatggc ttcagtcaca
     7021 tcatttaccc aacctcagct tcgttaatgg caactctcgg tgtgtgcagg gtggacttcc
     7081 gtaactggct gaaggtgggc gcgaccctac ttggactgct gtttattatg tccagcgtcg
     7141 tagtgatcgg cgctcagttg atgggctacc actaaaaatg ttaagagccg caattgcggc
     7201 tcttttttca ttctgccgtt tcagtctcta cggcttcatt tttggcattg cgcttcatcc
     7261 acaacgccag cgctttcagc gagtctggag tgaactcatc gcagcgtgcg gtgatttctt
     7321 ccggcgtcag ccagcaaact tcactgactt catcttcctg tagggcgaag ggaccgtgag
     7381 agacgcagct gaacaacgcg ccccagacac ggcaattttt atcttcgaaa tagaactgcc
     7441 cgtgctcggc aaaggggaca ccggcaatgc ccaactcttc ttccgcttcg cgacgcgcgg
     7501 attccagcag ttgctcatcg gcctgaacca caccgcctgc ggtcgcatct aacatgccgg
     7561 gtaaaaagtc ttttgtctcg gtacgacgct ggaccagaat tttgcccatg ccatcatgca
     7621 cgacgatgta agttgcacga tgacgcagac actgtgcccg catttgttcc cggctggctt
     7681 gtgcaatgac ttcgttctct tcattgacaa tatccaccca ttcagtactt gccaaacgac
     7741 gctgttccat tatcaggaaa ccttcttttt ctggcgctct tacggcgcat tttgagttgt
     7801 gggtaaatta cggattaatc gcgacctgcg caatgatact ttgatcattg agtgcgatta
     7861 cactaagtac gtcattatcc agcatgccat aactcgccgg attaccgcct ttcggaatac
     7921 tcaccgagcc ggggttgaag tggaaaatct ccccccgctg ttctgccact ggcagatggg
     7981 tatgaccgta caccagcaca tcgttctggt ttaaagcagg tagattttcc gggccaaaaa
     8041 gatgaccatg cgtcaaaaac agacgttgtt tttccagtaa tacctgttgc cacggcgcgg
     8101 ttatcgggaa atgcagcagc atttgatcca cttcgctgtc gcagttgccg cgcacagcga
     8161 taaccttatg tgccacttca ttaagccgtt cagcgacttt ggctggcgcg taaccctccg
     8221 gtaaagcatt acgcgggccg tgattcaaca cgtcgccaag gatcaccagc cactgggcac
     8281 cgctttgggc aaacaactcc agaacacgtt ccgtcgccgg taacgaccca tgaatgtccg
     8341 atgcaaacat cagtttcatc actcactcct cgtcgaaaaa acgtaagcta tgatactgga
     8401 ttctgtcgtt gctatcagcc agattgcttc gccgaaagtg caataaaacg ctggacagac
     8461 gctcgctgcc actggaatgt cgcataatcc accaggcgtt ctggcacctc atcgccatgt
     8521 agtaccaggc ggttaatcat tagcgccaga tcagtatcag caatgcacca ttcaccaaat
     8581 aaattcggct gacccagtgc taacaaatgt tctgccatcg cgacactggc ttttccctcg
     8641 gccgttagtg gcgctttttt cgcccccgca aagacaacat ccgtcggacg ctcttcgcgg
     8701 atgggcatca gatcgctgcg cagccaggcc tgaatctgtc gtgcacgcgc acgattttct
     8761 aaatcaagcg gataaatacg ttcccaggtc ggtggcgcaa atcgatcttc cagatactcc
     8821 gcaatggcag aagattcact caactcaaaa tcatcgattt gtaataacgg cacacggcgt
     8881 gtctgaccgt aaccttgcca cgtcggctgc aaatgttcac cgctgtcgag gtcgatggtc
     8941 ttgatatgaa acgacaggcc tttttcctgc aacgccaccc aagcggataa cacataaggg
     9001 gagaaaaagt gggcatctga ccaaagcgtg atagcgggtt tactcattat gtcctcggca
     9061 ataaaggagg ctggctatca aactatagag cctttttgtc actgtcacgt gacgaatact
     9121 cacgtaagtc gccgcgcttt ctatacttgt ggtgttcaga tgattaaaca accggagctg
     9181 caatgatcga tctctatttc gccccgacac ccaatggtca caaaattacg ctgtttctcg
     9241 aagaagcaga gcttgattat cgcttgatta aggtagacct ggggaaaggc ggtcagtttc
     9301 gtccggaatt tttgcgcatt tcgcccaaca acaaaattcc ggcaattgtt gatcattctc
     9361 ctgccgatgg cggcgaaccg ctaagcctct ttgaatctgg tgccattttg ttgtatctgg
     9421 ctgagaaaac ggggctcttt ttgagtcatg aaacgcgtga acgcgccgcc acattacagt
     9481 ggttattctg gcaggtaggc gaactggggc cgatgcttgg gcaaaatcat cattttaatc
     9541 acgcagcccc ccaaaccatt ccttacgcta ttgaacgtta tcaggttgaa actcagcgtc
     9601 tttaccatgt actaaacaag cggctggaaa actcgccctg gctgggaggc gagaactaca
     9661 gcattgcgga tattgcctgc tggccgtggg ttaatgcctg gactcgccag cgaattgacc
     9721 tcacaatgta tccggcagtc aagaactggc atgagcggat ccgttcgcgc cctgccaccg
     9781 ggcaggcact gctaaaagca caactcggtg atgagcgttc ggatagttaa cagaaacagg
     9841 ttctcgtgta ttatttcatc ctaagtaaaa caacggagaa cctgcaatgg cacaacctgc
     9901 cgctattatt cgtataaaga accttcgttt acgtacgttt atcggaatta aggaagaaga
     9961 aattaacaac cgtcaggata ttgttatcaa tgtgacgatc cactaccccg ccgataaagc
    10021 gcgcactagc gaagatatca acgatgcgct gaattatcgc accgtaacga aaaacattat
    10081 tcagcatgta gagaataacc gtttctcttt gttggaaaaa ttaactcagg atgtgctcga
    10141 tatcgcacgt gaacatcact gggtgacgta tgctgaagtg gagatcgata aactgcacgc
    10201 gctgcgctac gccgattcgg tatccatgac cttaagctgg cagcgttaac cgccatatcg
    10261 ggaggctgca tgaacatagt gatcaccgga gggacgggat taattggtcg ccatttgatt
    10321 ccgcgtttgc tggagctggg ccatcaaatc acggtagtga cgcgtaaccc acagaaagcc
    10381 agttccgttc tcggccctcg ggtgacgcta tggcaagggc tagccgatca aagcaacctc
    10441 aacggcgttg atgcggtaat caacctggcc ggagaaccga ttgctgataa acgctggact
    10501 cacgagcaaa aagagcgtct ctgccaaagc cgctggaata tcacgcaaaa actggtcgat
    10561 ttgattaatg ccagcgacac gccgccgtcg gtactcattt ccggctcggc aacgggctat
    10621 tatggcgact taggtgaagt ggtggttacc gaagaggaac cgccgcataa cgaatttact
    10681 cataaactct gcgcccgctg ggaagagatt gcctgccggg cgcaaagtga caaaacgcga
    10741 gtgtgcctgc tgcgaaccgg cgtggtactg gcaccggacg gcggtattct cggtaaaatg
    10801 ctgccaccgt ttcgtcttgg cctgggcggg ccgattgact ctggtcggca gtatctggcc
    10861 tggattcata ttgatgatat ggtcaacggt attctctggc tgctggataa cgagctgcgc
    10921 gggccattta atatggtttc gccctacccg gtacgcaatg aacaatttgc ccatgcgctc
    10981 ggtcacgcgc tgcatcgccc ggccattttg cgcgtccccg cgaccgccat tcgactgtta
    11041 atgggcgaat cttcagtact ggtattaggt ggacaacgcg cgctgcctaa acggctggaa
    11101 gaagcgggtt ttgcgtttcg ctggtacgat ttagaagagg cgctggcgga tgtcgttcgc
    11161 tgatgtggtt tacagcaaac atccgccagt taactcccgg tgttacagga ttagtggctt
    11221 tgcgcgataa gatcgtctgg tgaaagtcgg gtcaccatca taactaactc tctgtctaaa
    11281 cctctatcca gcatctcctg agcaatacgc agggcttctt cgtgtttgcc ctgcattgcg
    11341 ccttcttcac gtaatctgtc agcaatggtc atcagtttct ccttttcttg tggtgcgcgt
    11401 tccgctatct caccaataaa tgcacgaaaa cgctgggcat cccctgtttg taatacgtaa
    11461 ttaaacaggg cttttagctg tctgtcatta gtgttccctg taactagcag cgaaacaatt
    11521 tggtcgacta atcccaacag atcgcgctga cgaatatgtt tctgaattaa ctccaacagc
    11581 gccattttgc ggtgttgcat aatctcgtca tccggcacca cggtaatatc caccaacgga
    11641 aacgctgatg aatatatttt gcgggctata gcaggctcgg caaattcatc aagccagcag
    11701 agtgaataag gataaggact tctgcaacca tgataaaaca gcatcgggat caccaatgga
    11761 agctctttat agcccg
//
LOCUS       DAZPEB010000129        11751 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZPEB010000129 DAZPEB010000000
VERSION     DAZPEB010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11751
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.130"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(67..714)
                     /gene="ychE"
                     /locus_tag="ACHURJ_002953"
     CDS             complement(67..714)
                     /gene="ychE"
                     /locus_tag="ACHURJ_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFR1281822.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(745..798)
                     /gene="ychE"
                     /locus_tag="ACHURJ_002954"
     CDS             complement(745..798)
                     /gene="ychE"
                     /locus_tag="ACHURJ_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFR1281823.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            1192..3867
                     /gene="adhE"
                     /locus_tag="ACHURJ_002955"
                     /gene_synonym="adhC"
     CDS             1192..3867
                     /gene="adhE"
                     /locus_tag="ACHURJ_002955"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFR1281824.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGILSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            3991..4887
                     /locus_tag="ACHURJ_002956"
                     /pseudo
     CDS             3991..4887
                     /locus_tag="ACHURJ_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(5169..5786)
                     /gene="tdk"
                     /locus_tag="ACHURJ_002957"
     CDS             complement(5169..5786)
                     /gene="tdk"
                     /locus_tag="ACHURJ_002957"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFR1281825.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            6391..6804
                     /gene="hns"
                     /locus_tag="ACHURJ_002958"
     CDS             6391..6804
                     /gene="hns"
                     /locus_tag="ACHURJ_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFR1281826.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKARRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(6948..7856)
                     /gene="galU"
                     /locus_tag="ACHURJ_002959"
     CDS             complement(6948..7856)
                     /gene="galU"
                     /locus_tag="ACHURJ_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFR1281827.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEQVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(8058..9071)
                     /gene="rssB"
                     /locus_tag="ACHURJ_002960"
     CDS             complement(8058..9071)
                     /gene="rssB"
                     /locus_tag="ACHURJ_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFR1281828.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDATVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(9163..10068)
                     /gene="rssA"
                     /locus_tag="ACHURJ_002961"
     CDS             complement(9163..10068)
                     /gene="rssA"
                     /locus_tag="ACHURJ_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFR1281829.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQCREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGESLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            10181..10639
                     /locus_tag="ACHURJ_002962"
     CDS             10181..10639
                     /locus_tag="ACHURJ_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFR1281830.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            10689..11531
                     /gene="purU"
                     /locus_tag="ACHURJ_002963"
     CDS             10689..11531
                     /gene="purU"
                     /locus_tag="ACHURJ_002963"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFR1281831.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVNHEGLSRNEHDQKMADAIDAYK
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
BASE COUNT         3173 a         2931 c         2672 g         2975 t
ORIGIN      
        1 cgggaacaaa gtaaaaataa tgacgaagta aacggagcat ggcagctccg tttcatttga
       61 aaggaattaa ttaagcaggc cggggaaaat ccccttaata ccagtaacga taaattcaat
      121 ccccaatgcc atcagcaata gccccataat acgcgtaatc acgttgatgc cggtctggcg
      181 taaaacccgt accagccaag gtgccatgcg gaacaatccc cagcaacata aagcgaacaa
      241 tgcaatagcc acaaagaaac caaacagata gctaatgctg tgataacgcg taccccagac
      301 gatggtagaa ctgatcgccc ctggccccgc catcaacggc aacgccaatg gcaccacacc
      361 aatgctttca cgtaccgcgg tttctgattt ttcttgcttg ttctgtttat cctcgccaag
      421 cttgccgctg atcatcgaca tcgctatcgt caccaccagg atgcccccgg cgatacggaa
      481 cgaatcaatt gatataccaa aaagttgcag aatcgtgtcg ccgagaaaaa gcgagatcca
      541 caagataatg gccacagaca ggttggctgt aaggttagtt ttgtttcgcg ctgccgctgt
      601 ctgataactg gtcatgctga taaagacggg aataatccct accgggttga ccagcgcaaa
      661 taacccgatg aaaaatttga agtaaacggg aaaatcaaaa aaggtctgaa tcacggttag
      721 ctccgaagca aaagccggat aatgttagcc ataaataagg ttgaaaagac gcgctgacaa
      781 tacgcctttt gacagcattt ttcacctcct aactacttaa aattgctatc attcgttatt
      841 gttatctagt tgtgcaaaac atgctaatgt agccaccaaa tcatactaca atttattaac
      901 tgttagctat aatggcgaaa agcgatgctg aaaggtgtca gctttgcaaa aaatttgatt
      961 tggatcacgt aatcagtacc cagaagtgag taatcttgct tacgccacct ggaagtgacg
     1021 cattagagat aataactcta atgtttaaac tcttttagta aatcacagtg agtgtgagcg
     1081 cgagtaagct tttgattttc ataggttaag caaatcatca ccgcactgac tatactctcg
     1141 tattcgagca gatgatttac taaaaaagtt taacattatc aggagagcat tatggctgtt
     1201 actaatgtcg ctgaacttaa cgcactcgta gagcgtgtaa agaaagccca gcgtgaatat
     1261 gccagtttca ctcaagagca agtagacaaa atcttccgcg ccgccgctct ggctgctgca
     1321 gatgctcgaa tcccactcgc gaaaatggcc gttgccgaat ccggcatggg tatcgtcgaa
     1381 gataaagtga tcaaaaacca ctttgcttct gaatatatct acaacgccta taaagatgaa
     1441 aaaacctgtg gtattctgtc tgaagacgac acttttggta ccatcactat cgctgaacca
     1501 atcggtatta tttgcggtat cgttccgacc actaacccga cttcaactgc tatcttcaaa
     1561 tcgctgatca gtctgaagac ccgtaacgcc attatcttct ccccgcaccc gcgtgcaaaa
     1621 gatgccacca acaaagcggc tgatatcgtt ctgcaggctg ctatcgctgc cggtgctccg
     1681 aaagatctga tcggctggat cgatcaacct tctgttgaac tgtctaacgc actgatgcac
     1741 cacccagaca tcaacctgat cctcgcgact ggtggtccgg gcatggttaa agccgcatac
     1801 agctccggta aaccagctat cggtgtaggc gcgggcaaca ctccagttgt tatcgatgaa
     1861 actgctgata tcaaacgtgc agttgcatct gtactgatgt ccaaaacctt cgacaacggc
     1921 gtaatctgtg cttctgaaca gtctgttgtt gttgttgact ctgtttatga cgctgtacgt
     1981 gaacgttttg caacccacgg cggctatctg ttgcagggta aagagctgaa agctgttcag
     2041 gatgttatcc tgaaaaacgg tgcgctgaac gcggctatcg ttggtcagcc agcctataaa
     2101 attgctgaac tggcaggctt ctctgtacca gaaaacacca agattctgat cggtgaagtg
     2161 accgttgttg atgaaagcga accgttcgca catgaaaaac tgtccccgac tctggcaatg
     2221 taccgcgcta aagatttcga agacgcggta gaaaaagcag agaaactggt tgctatgggc
     2281 ggtatcggtc atacctcttg cctgtacact gaccaggata accaaccggc tcgcgtttct
     2341 tacttcggtc agaaaatgaa aacggctcgt atcctgatta acaccccagc gtctcagggt
     2401 ggtatcggtg acctgtataa cttcaaactc gcaccttccc tgactctggg ttgtggttct
     2461 tggggtggta actccatctc tgaaaacgtt ggtccgaaac acctgatcaa caagaaaacc
     2521 gttgctaagc gagctgaaaa catgttgtgg cacaaacttc cgaaatctat ctacttccgc
     2581 cgtggctccc tgccaatcgc gctggatgaa gtgattactg atggccacaa acgtgcgctc
     2641 atcgtgactg accgcttcct gttcaacaat ggttatgctg atcagatcac ttccgtactg
     2701 aaagcagcag gcgttgaaac tgaagtcttc ttcgaagtag aagcggaccc gaccctgagc
     2761 atcgttcgta aaggtgcaga actggcaaac tccttcaaac cagacgtgat tatcgcgctg
     2821 ggtggtggtt ccccgatgga cgccgcgaag atcatgtggg ttatgtacga acatccggaa
     2881 actcacttcg aagagctggc gctgcgcttt atggatatcc gtaaacgtat ctacaagttc
     2941 ccgaaaatgg gcgtgaaagc gaaaatgatc gctgtcacca ccacttctgg tacaggttct
     3001 gaagtcactc cgtttgcggt tgtaactgac gacgctactg gtcagaaata tccgctggca
     3061 gactatgcgc tgactccgga tatggcgatt gtcgacgcca acctggttat ggacatgccg
     3121 aagtccctgt gtgctttcgg tggtctggac gcagtaactc acgccatgga agcttatgtt
     3181 tctgtactgg catctgagtt ctctgatggt caggctctgc aggcactgaa actgctgaaa
     3241 gaatatctgc cagcgtccta ccacgaaggg tctaaaaatc cggtagcgcg tgaacgtgtt
     3301 cacagtgcag cgactatcgc gggtatcgcg tttgcgaacg ccttcctggg tgtatgtcac
     3361 tcaatggcgc acaaactggg ttcccagttc catattccgc acggtctggc aaacgccctg
     3421 ctgatttgta acgttattcg ctacaacgcg aacgacaacc cgaccaagca gactgcattc
     3481 agccagtatg accgtccgca ggctcgccgt cgttatgctg aaattgctga ccacctgggt
     3541 ctgagcgcac cgggcgaccg tactgctgct aagatcgaga aactgctggc atggctggaa
     3601 acgctgaaag ctgaactggg tattccgaaa tctatccgtg aagctggcgt tcaggaagca
     3661 gacttcctgg cgaacgtgga taaactgtct gaagatgcat tcgatgacca gtgcaccggc
     3721 gctaacccgc gttacccgct gatctccgag ctgaaacaga ttctgctgga tacctactac
     3781 ggtcgtgatt atgtagaagg tgaaactgca gcgaagaaag aagctgctcc ggctaaagct
     3841 gagaaaaaag cgaaaaaatc cgcttaatca gtagcgctgt ctggcaacat aaacggcccc
     3901 ttctgggcaa tgccgatcag ttaaggatta gttgaccgat ccttaaactg aggcactata
     3961 acggcttcca caacagggag ccgttttctt atgccacttc tcaatgatct gctcgatttc
     4021 agtgaccatc cgcttatgcc tccgccctct gcacaactat ttgcagaaca ccttcccacc
     4081 gagtggatac aacactgcct gacgctttct gctcatgcga ccgttcgccg ccgtcgttta
     4141 ccgggggaca tggttatctg gatggtggtg caatgagcca attaccgatg ttgttcgccg
     4201 tctgaacctg agcgcggatg gcgaagcggg gatgaacctg ctggcccgca gcgctgtcac
     4261 ccaagcgcgt cagcgcgtgg gggccgcccc agtggaatgg ctcttccgcc agaccgcaca
     4321 gacaggggcg cggaacgtta cctgaaggat gactggcacg gcctgcaact ttttgccatt
     4381 gatggcgcac agttcaggac acctgataaa cccgagctgc gtgaatatta tgggtctgcc
     4441 aacacatcca ctaagcggca gaacgcctac ccggttatgc gtctggtggc cttaatgaac
     4501 ctgggaagtc acattctgct gaatgccgtg accgcacctt accggcagag cgaaactgtg
     4561 ctggcccact ccatgctcgc caccatcccg gataactcta ttacgttgtt tgataagctt
     4621 ttttacagcg aagacctgct gctaacgctg aaccagaagg gttgtaaccg ccactggcta
     4681 ctgcctgcgt ggaagaatat cgcatcagaa atgatagagc tgggaaacac ggcgtcacca
     4741 gggaccatcc cgaaacgact ggagcatctt cggggtgctc tggaagtggt gttcataaca
     4801 aaacgccccc gaccatcaag gccgaggtcg gttaagatat caaaaacccg ttatcctgtg
     4861 aaacatagtg ccgctcctct taagtgaacg gcattgccct tctggggccg tattttttgt
     4921 ttacccaaag caacttttcc ataaaccgac agcattagcc ttcatcatat ttgcgacgat
     4981 gtataacgcc taaacacagg gatattgtac tttacaggtc acaagtcaac gtcggtgctt
     5041 aagagccctg tgaggcgtat agcggcgtta aaaaactgcc gagaagggta tatagcccgg
     5101 aagaagtgcg taaaacgaac tgacaggata aaagtgccct gctcaccctg tcagtaaaga
     5161 aattcttatt aatcgtggcg atgcctttcc tgaatagccg ttaatgagcc gacttgtaac
     5221 gcctctttat agtgtttacg gcatacagaa acgtatcgtt cattaccacc aattaccacc
     5281 tgctcacctt cgttataagg tctgcctgct tgatcaagac gcagcaccat gcttgcttta
     5341 cggccacaaa aacagatggt ttttaattca accagtttgt cggaccatgc cagtaagtat
     5401 tggctgccaa taaataattc acctcgaaaa tcggtacgta aaccataaca aagtacgggt
     5461 atatcgagtt gatcgacaac ctccgataat tcatatactt gttgtctggt taaaaactgg
     5521 cattcatcaa ccagtacgca atgaattgcc tgctgttcat gttccgcacg aatctcatca
     5581 aataatgatg aattttggtt aaataatttt gcaggcgatg acaaacctat acgcgaactg
     5641 actttcccgg caccaaagcg atcatcaatt tctgccgtat atacgacagt gcgcatgccg
     5701 cgttcttggt aattgtatga agattgcaac aatgctgtag acttacccgc attcattgcg
     5761 gaatagtaga aatatagctg tgccatcagc cacaggccct caatgatatg aaataaagtt
     5821 ggctggagtt tatcataatt cgtcagtttt tcagctcaac agtatgcaca gaagtgggta
     5881 tatgcgttct cccttacgaa gccttgcata atccttctga gctatcatta caactgcctc
     5941 gcttgttata agcgggtaaa tgactgctgg taactattca caatctttaa cctgttgcgc
     6001 aagtaatagc cctctgttga cctccaggag atagtgcaat actaagtcca tgctcttatt
     6061 gcgactgttc tacttttcat cattcgctta atagggaatt ctcgtaaaca caactaatac
     6121 agaagactga aaggccgtca gcctacgata atctccccat aaaatgtgac atgaatcagg
     6181 aagttttaac ctcacgtgct gcgaaatcat cggtgtaaat agggctatat gccgcgtctt
     6241 ttctggctaa ttttatgaaa agatatttat tggcggcaca aaataaagaa caattttgaa
     6301 ttccttacat tcctggctat tgcacaactg aatttaaggc tctattatta cctcaacaaa
     6361 ccaccccaat ataagtttga gattactaca atgagcgaag cacttaaaat tctgaacaac
     6421 atccgtactc ttcgtgcgca ggcaagagaa tgtacacttg aaacgctgga agaaatgctg
     6481 gaaaaattag aagttgttgt taacgaacgt cgcgaagaag aaagcgcggc tgctgctgaa
     6541 gttgaagagc gcactcgtaa actgcagcaa tatcgcgaaa tgctgatcgc tgacggtatt
     6601 gacccgaacg aactgctgaa tagccttgct gccgttaaat ccggcaccaa agctagacgt
     6661 gctcagcgtc cggcaaaata tagctatgtt gacgaaaacg gcgaaactaa aacctggact
     6721 ggccagggcc gtactccagc tgtaatcaaa aaagcaatgg atgagcaagg taaatccctc
     6781 gacgatttcc tgatcaagca ataatctttt gtagattgca attgctaaaa atcccgccat
     6841 cggcgggatt ttttattgtc cggtttaaga caatttaata agaataagct ataaaaaaac
     6901 ggcgtcgatt gttcaacgcc gtttcgtgga taacaccgat atggatgtta cttcttaatg
     6961 cccatctctt cttcaagcca ggctttaaat tccgtgccaa gggtgttatg acgaataccg
     7021 tattcaacga aggcctgcat gtaacctaat ttattaccgc agtcatggct cttccctttc
     7081 atatgatagg cttccaccgt ttctttttcg atcagcatat caattgcgtc ggtgagctga
     7141 atttcatcac cagctcccgg aggggttttt gccagcaacg gccaaatatc cgcgctaagt
     7201 acgtaacgac ccacaatagc gagattagac ggcgcaacat ccgctttcgg tttttctacc
     7261 acaccaacca tcggtacact ttcacccggc gctaattcaa cgcctttgca atccacaacg
     7321 ccatatgcgg tcacatcagc aacctgttca accatgatct ggctatgacc cgtttcatca
     7381 aagcggcgga tcatctctgc caggttatcc tgtgacaaat cggattcata ttcatccaga
     7441 ataacatcag gcaaaataac agctaccggt tcatcaccca ctaccgggtg agcacacaat
     7501 accgcgtgtc ccaggccttt cgccagcccc tgacgaactt gcataatagt cacgtgcggc
     7561 ggacaaatag actgcacttc atcaagcagt tgacgtttta cacgtttttc cagcattgct
     7621 tccagttcaa aactggtatc aaagtggttt tcaatcgagt ttttagatga gtgcgtaacc
     7681 agcacaattt cagtaatgcc agccgcaata cattcattca cgacgtattg aattaatggc
     7741 ttatcgacaa gtggcagcat ctctttcggg atggctttcg tcgccggcaa catcctggtt
     7801 cctaatcccg caacggggat aacggctttt ttgactttcg tattaatggc agccatttaa
     7861 attctcctgg actgttcgtg ttttgaacgt gttcatattt ctgtatcgca tcccagtata
     7921 tcagcacccc gaaggggcta agttctgaaa aggaggcgag ttagcataat taagacaatt
     7981 acttatacga atggcgacga tagtaccacc agccgacatt agcaggtaat gcaaatttag
     8041 tccgcgttat cgtttgctca ttctgcagac aacatcaagc gcagtcgacc accggttccc
     8101 catatttggc attgccaggc atcgcatcgc tggctcaatt gattcaaata agcgttacct
     8161 aaagtgccta acggaacacc attactgatt tgcacctggt gttcgccggt atttaacgtc
     8221 gcattcagac ccgcagaaac cagaatgaga tttttcagtt cgcgatgata atagccaact
     8281 aatagcggaa actgccccgg cagattggcc tgacggagta aatggtttac ctgcttcaat
     8341 aacgcgccca gctctggcaa ccgttgattt tggtgtgcaa gctgttcctg taataatccg
     8401 ttaaacaatg cgcgtaataa caaggcagca agtacgccat tatgtccagc tcgggtgaca
     8461 tcaaggcaat aaaatgccag atcgttttcc gaaagtgcgg caatatcaag caccaggccg
     8521 ggtttgtccg cggcaaccaa ttgacgataa ttaacccggc aatgggaaat cacctgctga
     8581 accggcggtt gtagttcctg taataatttc gccgctgcgg cagggttatc aaccgttgca
     8641 tcccaatcgc gaaaaagcct ttcctcttcc tcaacgcgcg aattaaacat gctgggatag
     8701 agacaggcaa aaaccatctc gcgcaagcga ttcagatctt taactggttt cagcaaaaca
     8761 tcttcaacgc ccagacgtaa cgctttggca atatctgcca tattttcagt ggcagatatc
     8821 accagaactg gggtctggtc gcctctgtta cgtatatgct ccagcagttt aagcccgttc
     8881 attcgtggca tcgcgatatc acatatcatc aggtctggag tgaaacctcc cagcaactca
     8941 agggcatcca ccccatcagc cgccagtacc gttgtcgctc ccaatgagga aaaccatgaa
     9001 tccagaagcg agcgaaatac ctgctcatct tcaacaatga gaatctgttt tccgaccaat
     9061 ggctgcgtca tgttctctcc cctgactggc tttactcaat agtggcatgc tattgccacc
     9121 tgcgcctgtc agaattaact taagtgtaga taaaaattct ggtcaaatgt tggtgcgtac
     9181 caacggcaaa agttcgtcca ttttcctttc cactgccagc tgtccggccg caatggcagc
     9241 gtgcgcgcga tggaaatcaa gcgtagatat ttgcgggcaa acaggttgaa tcagaatatc
     9301 gggcggatca cctgccatgc ggttcctttt aaggcggttc tccagcacct ggatagaagt
     9361 ggtcataatc tctgttgccg ttggcgctgt caccgcccga cgtgttgtta tactgcctaa
     9421 cctttctttc agacgcgcat gccacggcaa agattcacca ttctcgctat tttcttcact
     9481 gacattaaag gagagcaaat cttgttgcat caaatgagca tcgtgctgca ggtcgaccgc
     9541 tatcacaata tcagccccca atgcacgcgt gagggaaata ggaattgggt taactactgc
     9601 tccatcaacc agccagtagc cgttatgtgc aacaggtgcc atgagtcctg gaatactgca
     9661 tgatgcgcga atagcaagat ggagatcgcc ttcagtaaac cataattcac gtcccgtact
     9721 taaattggtg gcaacagccg caaagcgacg ggaacaattt tcgatctctg tttccggcat
     9781 tatttcgcgg cattgattga agacacgctc gccgcgcagt aacccaccgc gctgccagga
     9841 gagatccatc aggcgtaaaa catcccaata actgaaagag gtcacccaat cttccagcgc
     9901 agataatcga tcgcatgcat aggcagcgcc caccagcgaa ccaattgaac atcctgcaac
     9961 gatatcaatt tcaataccca ctttttttag cgcattaata acgccaatat gcgaccaacc
    10021 tctcgccgcg ccagatccca gcgccagccc tatttttatc tttctcatta ttcccgcaag
    10081 attcccctgg aatgcaatcg tagccacatc gctactgctc ggttaacata gtgctactca
    10141 ggcggttaat acgccgttat tgtttccagg gagattattt gtgtctcagc tttgtccctg
    10201 tggtagtgct gtcgagtata gcctatgttg ccacccttat gtgtctggtg aaaaggttgc
    10261 acctgatcca gaacatctca tgcgttcgcg ttactgcgct tttgtgatgc aagacgcaga
    10321 ttatttaata aagacctggc atccctcttg tggagcagcg gcgttacgtg ccgaattgat
    10381 ggccggattt gcgcatactg aatggttggg attgaccgta tttgaacatt gttggcagga
    10441 cgccgacaat atcggatttg tcagttttgt tgctcgattt accgaaggtg gcaaaacagg
    10501 tgcaataatt gaacgttcgc gatttttgaa agaaaacggt caatggtact atattgacgg
    10561 tacacgtccg cagtttggtc gcaacgatcc ctgcccttgt ggttcaggta aaaaatttaa
    10621 aaagtgctgc ggccaataat ggttgacggt acggtttagc aaacactctc aacaaggttt
    10681 ttccagcaat gcattcactc caacgtaaag ttctgcgtac tatttgtccg gaccaaaaag
    10741 gtctgatcgc acgtattacc aatatttgct acaagcacga gttaaatatc gtacagaaca
    10801 atgaatttgt tgatcaccgt accgggcgct tttttatgcg cacggaactg gaagggattt
    10861 ttaatgattc caccctgctg gcggatctcg atagcgcatt gccagaaggc tccgtgcgtg
    10921 agctgaatcc tgccggtcgt cgccggatag tgattctggt cactaaagaa gcgcattgcc
    10981 ttggcgattt gttgatgaaa gccaattacg gcggcctgga tgtcgaaatc gcggcagtta
    11041 ttggtaacca cgatacttta cgttctctgg ttgagcgttt tgatattccg tttgagctgg
    11101 taaaccatga agggttaagc cgcaacgagc acgatcaaaa gatggcagac gccattgatg
    11161 cttataaacc tgactatgtg gtgctggcga agtatatgcg ggtattaacg ccggaatttg
    11221 tggcacgctt cccgaataaa atcatcaata ttcaccattc attcctgcct gcgtttatcg
    11281 gcgcacgtcc ttatcaccag gcctatgaac gtggtgtgaa gattattggc gcaaccgctc
    11341 actatgtgaa tgacaatctg gacgaaggcc caatcatcat gcaggacgtt attcatgtcg
    11401 atcataccta cacagctgaa gatatgatgc gcgcaggtcg tgacgtcgag aaaaacgtct
    11461 taagtcgtgc gctatacaaa gtactggcac agcgcgtctt tgtttacggt aatcggacga
    11521 ttattcttta atcgccagca aaaataactg gttaccttta atccgttacg gatgaaaatt
    11581 acgcaaccag ttcatttttc tcaacgtaac actttacagc ggcgcgtcat ttgatatgat
    11641 gcgccccgct tcccgataag ggagcaggcc agtaaaaagc attaccccgt ggtggggttc
    11701 ccgagcggcc aaagggagca gactgtaaat ctgccgtcat cgacttcgaa g
//
LOCUS       DAZPEB010000130        11701 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZPEB010000130 DAZPEB010000000
VERSION     DAZPEB010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11701
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.131"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            154..312
                     /locus_tag="ACHURJ_002964"
                     /pseudo
     CDS             154..312
                     /locus_tag="ACHURJ_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
     gene            299..886
                     /locus_tag="ACHURJ_002965"
     CDS             299..886
                     /locus_tag="ACHURJ_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001214489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1281832.1"
                     /translation="MRGLENAIRNLNSLDTRMVPQASAWAVNRVAAKVVSVATRQVAR
                     NTVAGDNQVKGGPLKLVRQRVRVFKASPAGKMYARIRVNRGNLPAIKLGTTCRRAGDG
                     LKVGKYFFRGAFIQQLANGRWHVLRRLPEACFETGKDSKGRPRKNRLPVEVVKIPLAG
                     PLTQAFEDARDRIIAAEMPKQLGYALKQQLRIHLG"
     gene            883..1284
                     /locus_tag="ACHURJ_002966"
     CDS             883..1284
                     /locus_tag="ACHURJ_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFR1281833.1"
                     /translation="MNRHTQIRQAVLASLKGECGGNVVLFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFVRAQAPDSELDTWMENTIFPAMKDIPVLSGLID
                     TMIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            1295..2035
                     /locus_tag="ACHURJ_002967"
     CDS             1295..2035
                     /locus_tag="ACHURJ_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1281834.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGKGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTLATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTITSKEVMTRTVKISGVGRPYLAEEGAETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKG
                     VKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            2094..2480
                     /locus_tag="ACHURJ_002968"
     CDS             2094..2480
                     /locus_tag="ACHURJ_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFR1281835.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            2489..2817
                     /locus_tag="ACHURJ_002969"
                     /pseudo
     CDS             2489..2817
                     /locus_tag="ACHURJ_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308863.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
     gene            2789..5830
                     /locus_tag="ACHURJ_002970"
     CDS             2789..5830
                     /locus_tag="ACHURJ_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR1281836.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAAGDAERSSARMQ
                     RFMERQTQAARQTMHAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGVGQQ
                     DYLALISEITAKTRALTQAEEQATRQKAAFIRQLKEQATRQNLSSSELLRARAAQLGV
                     SSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGNFGALRGSGITLANRA
                     GWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEEFNRQLALTGHYAGVTVG
                     QLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNALHFLTATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     KRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQIDALQEKIQRAKKYP
                     WTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTRATRNDEATRLLLQYS
                     QQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLARKN
                     ELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERALREQHNLDIATAGMGD
                     KQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEEVLKGSLKRQLNENKR
                     YWQELEVAQGDWKNGAMRAFQNFTADADNAVGTAEQMFTVAFSSAGNALATFCTTGKL
                     NFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGVYQSADLSRYSGTVVN
                     RPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSGMVMFAPQYNIGINND
                     GTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            5830..6159
                     /locus_tag="ACHURJ_002971"
     CDS             5830..6159
                     /locus_tag="ACHURJ_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1281837.1"
                     /translation="METFRWKVRPDMNVVSEPKVVAVKLGDGYEQRRPAGLNNLLSTY
                     SVTIRVRKGEHPPLKAFLERHGGVRAFQWTPPYDWKPIRVACRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            6159..6858
                     /locus_tag="ACHURJ_002972"
                     /pseudo
     CDS             6159..6858
                     /locus_tag="ACHURJ_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
     gene            6863..7606
                     /locus_tag="ACHURJ_002973"
     CDS             6863..7606
                     /locus_tag="ACHURJ_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR1281838.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGERYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            7570..8145
                     /locus_tag="ACHURJ_002974"
     CDS             7570..8145
                     /locus_tag="ACHURJ_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFR1281839.1"
                     /translation="MQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSDGWYQVRIAG
                     RDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVAIWMPGVGIM
                     ASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMVAQGNVLPVL
                     YGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            8206..11685
                     /locus_tag="ACHURJ_002975"
     CDS             8206..11685
                     /locus_tag="ACHURJ_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1281840.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGVESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVENTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCR
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNVVEVNWIDPDNGHETATELVEDAQAILRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSCTTLISLVDGNGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITAVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDDAEGYLSFYKGLINNTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCSLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKATAGITIS"
BASE COUNT         2751 a         2763 c         3749 g         2438 t
ORIGIN      
        1 ccatttgaag tttaaactca tttgaaaatg atctgcgggg ttctgcagat aatgctttct
       61 gttccataac aggtgtccac tagttgaaaa ggtgggcacc tacgttacca atacaggctt
      121 aatggctaca tacggcggtc agtttacgct tacgatattt tacagctgtg tcgcaatgat
      181 tcgctgatga ttggccggga gtctttcagc gtggatcgta ttactcctga tgacggcgga
      241 tgtagttata tccggttgcg gcgtgaagga gcaggaaacg taagggcagg gggatattat
      301 gagggggctt gagaatgcca tccgcaatct gaacagtctt gatacccgta tggtgccgca
      361 ggccagcgca tgggcagtaa accgggttgc tgcaaaagtt gtctcggttg ccacccggca
      421 ggtcgccagg aataccgttg cgggagataa ccaggtgaag gggggccccc tgaaactggt
      481 gcgtcagcgc gtccgggtat ttaaagccag tccggcagga aaaatgtatg cccgtatccg
      541 tgttaaccgg ggcaatctgc ccgccattaa gcttggcaca acatgccggc gtgccggtga
      601 cggactgaag gtggggaaat acttttttcg tggtgcgttt atccagcagc tggcgaacgg
      661 tcgctggcat gttctcaggc gtcttcctga agcgtgtttt gaaacaggaa aggacagtaa
      721 ggggcggccc agaaaaaatc gtctgcccgt ggaggttgtg aaaattccgc tggccggacc
      781 gctgacacag gcatttgagg atgcccgcga tcgcatcatt gccgcggaaa tgccgaaaca
      841 gctggggtat gccctgaaac agcaactgag gatacatttg ggatgaacag gcacacacaa
      901 atccgccagg cagtactggc aagcctgaag ggcgaatgcg gggggaatgt cgtgcttttt
      961 gacgggcttc cggcatttat tgatgcgcag gaactgcccg ccgtggcggt gtggctgagt
     1021 gatgcgcagt acaccggaaa aatgacggat gaggacgact ggcaggctgt tctgcatatt
     1081 gccgttttcg tccgtgcaca ggcgccggat tctgagctgg atacgtggat ggagaacacc
     1141 atatttccgg ccatgaagga tattccggtg ctttccggac tcatcgacac catgatccca
     1201 ctcggtttta actatcaacg tgataatgag atggccacct gggcgatggc ggaaatcacg
     1261 taccagatca cgtacacgaa ttaaggaggt ggcaatgacc acaccaaatc cactggcaaa
     1321 gacgaaaggt gcgggaacga cgttctggat gtacaccggc aagggcgatg cgtttgcgaa
     1381 ccctttgtcg gacactgact ggctgcgtct tgcgatggtg aaggatctgc aacctggcga
     1441 aatgaccgct gatgcagaag atgacactta tctcgatgat gaagatgcag actggaaaac
     1501 gacaacccag gggcagaaat ccgtcggtga tactttggcg acgctggcct ggcgtccggg
     1561 tgacagcggg cagaaaaaac tggttcagtt gttcgattcc ggtgaagtct gcgcgtttcg
     1621 tatcaaatac cccaacggta cggttgatgt tttccgtggc tggctgagtt cactgggtaa
     1681 aaccattacc tcaaaagagg tgatgacccg tactgtaaaa atcagcggtg tggggcgtcc
     1741 gtatctggca gaggagggcg ctgaaaccgt gggtgttacc ggactgacgg tggcaccggc
     1801 atctgtcagt gtcaaagcgg gagcaaccac cacactgacc tttacagtaa aacctgacgg
     1861 ggccagtgac aaagcgatca gtgtgcattc gtcagatcct cagactgctt cggtgaccct
     1921 gagcgggctt gtggccacgg tgaaaggcgt gaagcagggc agtgtcagca tcgtgggcat
     1981 gacctctgac ggagagtttg tggcagtggc tgcggtgacc gtcagcgcac cataacagga
     2041 cgatactcat cattgccccg gttatccggg gctttttgca tccggagaac atgatgtttc
     2101 tgaaacagga tacgtttaat tatgaaaaac agtccgtggt gctcagtgag ctgtccgggc
     2161 tgcagagaat tgaatatctg acgtttgttc agcagcgaac ggcaaagttt gatgcacagg
     2221 agggagaact gccggaggct gaacgacaga ttgcttttct gcgtatggga atggatatca
     2281 atgcctggct ggtttcccgc tcactgtgga atgctgagca gtctcaggat gttgagacgc
     2341 tttgcgcatc cattatgaca acatggtcgt atgatgcgct gggcgcgggg gcggagaggg
     2401 ttctgtcgct gagcggtatg gggaccattg agaatgccgg ggatgatgat catgaggcgc
     2461 tgacgccgga aaagtcctga cgcgggaaat gcagtttgtc atgcggcttg cccgggagtt
     2521 ccggcgggca gactggcggc ggatgctgtc ggaaatgtcg gccactgagc ttggtgaggg
     2581 ggcgattatt tccggatgca gagcttcagt gatgtgtgga tggatgcgca gtttgcctcg
     2641 ctgaaggcat tgatcgtgag aatggtgtcc ggcagcagtg atgctgcggt ggctgatttc
     2701 agccttttac cggaagagaa cgggataccg gagcgaacgg acgaagaact gatgcatctt
     2761 ggggaaggta tttccggagg tgtgcgttat ggaccagata gccaacctgg tcattgattt
     2821 ggggattgat gcggcagagt ttaaaaatga aattccccgt atcaaaaacc ttctgaatgg
     2881 tgcagccggt gatgcagaac ggtcttctgc ccgtatgcag cgttttatgg agcgtcagac
     2941 tcaggcggcc cggcagacaa tgcatgcggc ttcttcggct gcaacagcag catcagccca
     3001 tgcgcagacg gtggagaaga atgcacgggc tcatgaacgc atggcccgtg aggtggaaca
     3061 aacccgtctg cgcgtggatg ccctgaatca gaaaatgcgc gaggaacagg cgcaggccag
     3121 ggcactggcg gaggcgcagg ataaagcggc tgccgccttt tatcgccaga ttgacagtgt
     3181 gaaacaggcc ggtgcggggc ttcaggaatt acagcgtatt cagcagcaga tccgacaggc
     3241 cagaaacagt ggcggggttg gtcagcagga ttatctggcg ctgatttcgg agatcacggc
     3301 gaaaacccgt gccctgacgc aggcagagga acaggccacc cggcagaaag cggcgtttat
     3361 ccgccagctt aaagagcagg caacccgcca gaatctgtcg tcttctgagt tgcttcgcgc
     3421 cagggcggct cagctggggg taagcagtgc tgcagaagtg tatatccgca aaatggagcg
     3481 ggcaggaaaa gccacacatt cgctggggct gaaaagtgcg gcagcccggc gggagctggg
     3541 ggtgttaatc agtcagatgg cgcgcggcaa ttttggtgcg ctgaggggat ccgggataac
     3601 gctggctaac cgtgccggat ggacaggcgc actgatgtcg ccgaaaggca tgatgactgg
     3661 cggcgttatt ggcggacttg tcgcggcggt cctgggtctg ggtaaagcct ggcatgacgg
     3721 ccggaaggag ggcgaggaat ttaaccgtca gctggcgctg acgggacatt atgccggtgt
     3781 cactgtcggg cagttgtgga aactcagccg ggccatatcc gggaatggta tcacgcaaca
     3841 tgcggcagcc ggtgcgctgg cacaggtggt gggcagtggg gcatttcatg gaaacgatat
     3901 tggtatggtg gcgaaagctg ccgcacagat ggagcgatcg gttggccagt cggtcagcga
     3961 taccataaat cagtttaagc ggctgaagga tgatcctgta aatgccgcga aggcgctgga
     4021 caatgcgctg cattttttga ctgccactca gcttgagcag atacgcgtcc ttggggagca
     4081 ggggcggtcc agtgatgcgg cccggatcgc catgtctgcg ctggcagagg aaaccggtaa
     4141 acgcacatct gatattgata ataatctcaa tgcgctgggt agtacgctgc aaaccttgtc
     4201 tgactggtgg aagcagttct gggatgcggc catgaatatt ggtcgtgaag actcgctgga
     4261 tgcgcagatt gatgcgttac aggaaaaaat tcagcgcgcg aaaaaatatc cgtggacaaa
     4321 cgcctccaca caggtggagt acgatcagca gcgtcttaac gatcttcagg agaaaaaacg
     4381 ccggaaggat ttgcaggatg caaaagcgca ggcagaacgg aattaccagg agcaacagaa
     4441 acgccggaat gctgaaaatg ccgcgctgaa ccggatgaat gaaacggaag ctgcacgaca
     4501 tcagcgggaa attgcgcgta ttaatgccat gcagtacgcc gaccaggctg tcagggatgc
     4561 ggcgatacag cgtgaaaatg aacgttacga aaaagccatt aagaaaaata cacgggcaac
     4621 ccgtaatgat gaggccaccc ggttattgct gcagtacagt cagcagcagg cacaggtgga
     4681 agggcagatt gctgctgcca gacagtcagc aggcattgcc actgaaagga tgactgaagc
     4741 gcataaacag cttctggccc tgcagcagcg catcagcgac ctggacggga aaaagctgac
     4801 ggcagatgaa aagagtgtgc tggcccgtaa gaatgagctg attcaggcgc tgacgctgct
     4861 ggatgtgaaa cagcaggagc tgcagaaaca gacagcgctt aacgacctga gaaaaaaaac
     4921 ggttcagctg accagccagc tggcagacaa agaacgtgca ctgcgtgagc agcacaatct
     4981 ggatattgcc actgcaggta tgggggataa gcagcggcag cgctaccagg cacagttgcg
     5041 catccggcag gaataccggc aacagttgca acagcttgag aatgacagtc gccagaaagg
     5101 cacttacggg acggaggact accggagggc tgaggaggtg ctgaagggga gcctgaagcg
     5161 acaactgaat gaaaacaaac gctactggca ggaactggaa gtggcgcagg gcgactggaa
     5221 aaacggtgcc atgcgggcgt ttcagaattt tacggcggat gcggataatg cggtgggaac
     5281 ggcagaacag atgtttacag tggcattcag cagtgccggt aatgcactgg cgacattctg
     5341 taccaccggt aagctgaatt ttaaatcctt cacctcttcc ctgttgtcag atatggccag
     5401 aattatggca cagatggcca tgatgcaggc ggtaaagggc gtcggctctt tattcggctt
     5461 cacgactaat gctgatggcg gtgtttacca gtctgctgat ttgagtcgct acagtggcac
     5521 ggtggttaac cgtccgacgt tttttgcttt tgcaaaaggc gcgggtgtga tgggggaagc
     5581 gggacctgaa gccattctgc cactgcgtcg cggtgctgat ggtaagctgg gggttgtggc
     5641 ggatactggt ggttcaggca tggtgatgtt tgccccgcag tacaacatcg ggatcaataa
     5701 cgacggcacg aacgggcaga taggtccggc agcactgaag gtggtttatg acctcgggaa
     5761 aaaagcggcc gcggacttta tgcaacagca ggcccgtgat ggtggccggt taagtggagc
     5821 ataccggtaa tggagacgtt tcgctggaag gtgcgcccgg atatgaatgt ggtatcagag
     5881 ccaaaagtgg tggcagtgaa gctgggtgat ggttatgaac agcgtcgccc ggcgggactg
     5941 aataatctgc tgtcgactta cagcgtgacg atacgtgttc gtaaaggtga acacccacct
     6001 ttaaaagcct ttctggaacg gcacggtggc gtccgcgcat ttcagtggac gccaccttat
     6061 gactggaaac cgatcagggt ggcttgtcgt aaatggtcgg caagcgtggg ggcgttatgg
     6121 gtgactgtaa cggccgattt tgaacaggtg gtgaactgat gcaggatatc cggcaggaaa
     6181 cactgaatga atgcacccgt gcggagcagt cggccagcgt ggtgctctgg gaaatcgacc
     6241 tgacagaggt cggtggagaa cgttattttt tctgtaatga gcagaacgaa aaaggtgagc
     6301 cggtcacctg gcaggggcga cagtatcagc cgtatcccat tcagggggag tggttttgaa
     6361 ctgaatggca aaggcaccag tacgcgcccc acgctgacgg tttctaacct gtacggtatg
     6421 gtcgccggga tggcggaaga tctgcagagt ctggtcggcg gaacggtggt ccggcgtaag
     6481 gtttacgccc gttttctgga tgcggtgaac ttcgtcaacg gaaacagtga cgccgatccg
     6541 gagcaggagg tgatcagccg ctggcgcatc gagcagtgca gcgaactgag cgcggtcagt
     6601 gcctcttttg tgctggccac accaacggag acggatggcg cggttttccc ggggcgtatc
     6661 atgctggcga atacctgtat gtggacctac cgttctgatg agtgtggtta cacgggcagg
     6721 gcagtggctg acgagttcga caaaccaacg acggatatcc ggaaggacaa atgcagcaag
     6781 tgtatgcgcg ggtgtgagtt gcgcaacaat accggtaatt tcggcggttt cctttccatc
     6841 aataaacttt ctcagtaaat ccatgacaca gacagaatca gcgattctgg cgcacgcccg
     6901 gcgatgtgcg ccagcggagt cgtgtggctt cgtggtgaga acgccggagg gggagcggta
     6961 tatcccttgt gtgaatattt ctgcagagcc ggaggcgtat tttcgtatcg caccggaaga
     7021 ctggctgcgg gcagagatgc agggggagat tgtggcactg gtccacagtc atcccggtgg
     7081 tctgccctgg ctgagtgagg ctgaccggcg gctgcaggtg cagagtgatt tgccgtggtg
     7141 gctggtctgc cggggggcga ttcacaagtt ccgctgtgtg ccgcatctca ccgggcggcg
     7201 ctttgagcac ggggtgacgg actgttacac gctgttccgg gatgcttatc atctggcggg
     7261 gattgagatg ccggattttc atcgcgggga tgactggtgg cgtcacggtc agaatctcta
     7321 tctggataat ctggaggcca cagggctgta tcaggtgccg ttgtcatcag cacaaccggg
     7381 cgatgtgctg ctgtgctgtt ttggttcatc ggtgccgaat catgccgcca tttactgcgg
     7441 cgacggtgag ctgctgcacc atattcctga acaactgagt aaacgagaga ggtataccga
     7501 caaatggcag cgacgcacac actccctctg gcgtcaccgg gaatggcacg catctgcctt
     7561 tacggggatt tgcaacgatt tggccgccgc atcgaccttc gtgtgaaaac gggggctgaa
     7621 gccatccgcg cactggccac acagctcccg gcgtttcgtc agaaactgag tgacggctgg
     7681 tatcaggtgc gcattgccgg gcgtgatgca ggtgaaaccg aattgtctgc ccgtcttaat
     7741 gagccgctgg caaatggtgc agtgatccac atagtaccgc gtctggtggg agctaaaagt
     7801 ggcggtgtgt ttcaggcggt gctgggggca gctgttatgg cggttgctat atggatgccg
     7861 ggggtaggaa ttatggcgag taatctgctg ttttctctcg gtgccagtat gacgcttggc
     7921 ggtgttgcac agatgctggc accgaaagcc agaactcccc gtacacagac aacggataac
     7981 ggcaaacaga acacctgttt ctcctcactg gataacatgg ttgcccaggg caatgttctg
     8041 cctgttctgt acggtgaaat gcgcgtgggg tcgcgggtgg tatctcagga gatcagcacg
     8101 gcagacgaag gggatggtgg tcaggttgtg gtgattggtc gctgatgcaa aatgtttcat
     8161 gtgaaaccgc ctgcgggcgg ttttgtcgtt tatggagcgt gaggaatggg taaaggcagc
     8221 agtaaggggc ataccccgcg cgaagcgaag gacaacctga aatccacgca gttgctgagt
     8281 gtgattgatg ccatcagcga agggccggtt gacggtccgg tggatggatt aaaaagcgtg
     8341 ctgctgaacg gtacgccagt cctggacagc gaggggaaga caaacttttc cggtgttacg
     8401 gtggtgttcc gcgccggcga gcaggagcag acaccgccgg agggggttga atcttccggc
     8461 tcagagactg tgctgggtac ggaagtgaaa tacgacacgc cgatcacccg gaccatcacg
     8521 tcggcaaaca ttgatcgtct gcgcctgacc ttcggtgtgc aggcactggt ggaaaacacc
     8581 tcaaaggggg accggaatcc gtcggaagtc cgcctgctgg ttcagataca gcgtaacggt
     8641 ggctgggtga cggaaaaaga catcaccatt aagggcaaaa ccacttcgca gtatctggcc
     8701 tcggtggtgg tgggtaacct gccgccgcgc ccgtttaata tccggatgcg caggatgaca
     8761 ccggacagca ccacagacca gctgcagaac aaaacgctct ggtcgtcgta caccgaaatc
     8821 atcgatgtga aacagtgcta cccgaacacg gcgctggtcg gcgtgcaggt ggattcagag
     8881 cagttcggta accagcaggt gagtcgcaat tatcatcttc gcgggcgcat tctgcaggtg
     8941 ccgtcgaact ataacccgca gacgcggcaa tacagcggta tctgggacgg aacgcttaag
     9001 ccagcataca gcaacaacat ggcctggtgt cggtgggaca tgctcactca tccgcgctac
     9061 ggcatgggga aacgtcttgg tgcggcagat gtggacaaat gggcgctgta tgtcatcggc
     9121 cagtactgcg accagtcagt gccggacgga tttggcggca cggagccgcg catcacctgt
     9181 aacgcttacc tgaccacaca gcgtaaggcg tgggatgttc tcagtgattt ctgctcggcg
     9241 atgcgctgta tgccggtatg gaacgggcag acgctgacgt tcgtgcagga ccgaccgtcg
     9301 gataaagtgt ggacctataa ccgcagtaat gtggtgatgc cggatgatgg cgcgccgttc
     9361 cgctacagct tcagcgccct gaaggaccgc cataatgtcg ttgaggtgaa ctggattgat
     9421 ccggacaacg gtcatgagac ggcgacagag cttgtggagg acgcgcaggc cattctccgt
     9481 tacggtcgta acgtcacgaa gatggatgcc tttggctgta ccagccgggg acaggcgcac
     9541 cgcgccgggc tgtggctgat taaaacggaa ctgctggaaa cgcagaccgt ggacttcagc
     9601 gtcggcgcag aagggcttcg ccatgtaccg ggtgatgtca ttgaaatctg cgatgatgat
     9661 tatgccggta tcagcaccgg tggtcgcgta ctggcggtga acagccagac ccggacgctg
     9721 acgctcgacc gtgaaatcac gctgccgtcc tcctgtacca cgctgataag cctggttgac
     9781 ggaaatggca atccggtcag cgtggaggtt cagtccgtca ccgacggcgt gaaggtgaaa
     9841 gtgagccggg ttcctgacgg cgttgcagaa tacagcgtgt gggggctgaa gctgccgacg
     9901 ctgcgccagc gcctgttccg ctgcgtgagt atccgtgaga acgacgacgg cacgtatgcc
     9961 atcaccgccg tgcagcatgt gccggaaaaa gaggccatcg tggataacgg ggcacacttt
    10021 gacggtgacc ggcgcggcac ggtgaatggt gtcacgccgc cagcggtgca gcatctgacc
    10081 gccgaagtca ccgcagacag cggggaatat caggtgctgg cgcgatggga cacgccgaag
    10141 gtggtgaagg gtgtgagctt cctgcttcgc ctgaccgtgg cagcggacga cggcagtgag
    10201 cggctggtca gcacagccag gacgacggaa accacatacc gcttcaggca gctggcgctg
    10261 gggaattaca gtctgacagt ccgggcggta aatgcccggg ggcagcaggg cgatccggcg
    10321 tcggtatcgt tccggattgc ggcaccggca gcgcctgtca ctattgaact gataccgggg
    10381 tattttcaga taacggcggt cccgaaactg gctgtatacg acccgacggt gcagtttgag
    10441 ttctggtttt cggaaaagcg gattgcggat atcaggcagg ttgaaaccag cgcccgctat
    10501 cttggcacgg cgctgtactg gatagccgcc agtatcaata tcaggccggg ccatgattat
    10561 tatttttacg ttcgcagtgt gaacaccgtt ggcaaatcgg catttgtgga ggccgtcggt
    10621 cgggcgagcg atgatgcgga aggttatctg agtttttata aagggttgat caataacacg
    10681 catctcggca aggagttgtg gacgcagatt gataacggtc agcttgcgcc ggacctgact
    10741 gaaatcagga cgtccattac gaatgtcagc aatgaaatca cgcaaaccgt caataaaaaa
    10801 ctggaaaatc agagtgctgc aatccagcag atacagaaag ttcaggttga tacaaataat
    10861 aacctgaaca gcatgtgggc tgtgaagctg caacagatga aggacggacg cctttatatt
    10921 gcgggtatcg gtgccggtat tgagaatacg ccagcaggaa tgcagagtca ggtgctgctg
    10981 gcggcagaca ggattgcgat gattaatcct gcgaatggca acacaaagcc gatgtttgtt
    11041 ggtcagggcg atcagatatt catgaacgac gtgttcctga aacgcctgac ggctccgacc
    11101 attaccagcg gcggtaatcc tccggcattt tccctgacac ctggcggacg gctgacggcg
    11161 aaaaatgccg atatcagcgg taacgtgaac gcgaactccg ggacgctcaa caacgtcacg
    11221 attaataaga actgtcgggt tctgggaaaa ttgtccgcga accagattga aggcgatctc
    11281 gttaaaacag tgggcaaacc tttcccacgg gactcccggg caccggagag gtggccatca
    11341 gggaccatta ccgtcagggt ttatgacgat cagccgtttg atcggcaaat tgttattccc
    11401 gcggtggcgt ttcgcggtgc taaacatgag cggaagaata acaatattta ttcgtcatgc
    11461 agcctgatag tgaagaaaaa cggtgctgaa atttataacc gaacgaccct ggataatacg
    11521 ctgatatata cgggtgttat tgatatgcct gccggtcacg gtcacatgac gctggagttt
    11581 tctgtatcgg catggctggt aaatggctgg tatcccacag caagtatcag cgatttgctg
    11641 gttgttgtga tgaagaaagc cactgcaggc atcacgatta gctgaatttt ataacccaga
    11701 t
//
LOCUS       DAZPEB010000131        11614 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZPEB010000131 DAZPEB010000000
VERSION     DAZPEB010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11614
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.132"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(37..1023)
                     /locus_tag="ACHURJ_002976"
                     /pseudo
     CDS             complement(37..1023)
                     /locus_tag="ACHURJ_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310582.1"
                     /note="PtrB; oligopeptidase that cleaves peptide bonds
                     following arginine and lysine residues; incomplete;
                     partial in the middle of a contig; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
     gene            complement(1232..1891)
                     /locus_tag="ACHURJ_002977"
     CDS             complement(1232..1891)
                     /locus_tag="ACHURJ_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFR1281841.1"
                     /translation="MANWLNQLQSLLGQSSSSISSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(2218..2574)
                     /gene="yebF"
                     /locus_tag="ACHURJ_002978"
     CDS             complement(2218..2574)
                     /gene="yebF"
                     /locus_tag="ACHURJ_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFR1281842.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(2641..2935)
                     /gene="yebG"
                     /locus_tag="ACHURJ_002979"
                     /pseudo
     CDS             complement(2641..2935)
                     /gene="yebG"
                     /locus_tag="ACHURJ_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            3069..4247
                     /gene="purT"
                     /locus_tag="ACHURJ_002980"
     CDS             3069..4247
                     /gene="purT"
                     /locus_tag="ACHURJ_002980"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFR1281843.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEERLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVADIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWKYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCSDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(4303..4944)
                     /gene="kdgA"
                     /locus_tag="ACHURJ_002981"
                     /gene_synonym="eda"
     CDS             complement(4303..4944)
                     /gene="kdgA"
                     /locus_tag="ACHURJ_002981"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFR1281844.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(4981..6792)
                     /gene="edd"
                     /locus_tag="ACHURJ_002982"
     CDS             complement(4981..6792)
                     /gene="edd"
                     /locus_tag="ACHURJ_002982"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFR1281845.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQSPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(7027..8502)
                     /gene="zwf"
                     /locus_tag="ACHURJ_002983"
     CDS             complement(7027..8502)
                     /gene="zwf"
                     /locus_tag="ACHURJ_002983"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFR1281846.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            8840..9709
                     /gene="hexR"
                     /locus_tag="ACHURJ_002984"
                     /gene_synonym="yebK"
     CDS             8840..9709
                     /gene="hexR"
                     /locus_tag="ACHURJ_002984"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFR1281847.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            9837..11279
                     /gene="pyk"
                     /locus_tag="ACHURJ_002985"
     CDS             9837..11279
                     /gene="pyk"
                     /locus_tag="ACHURJ_002985"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFR1281848.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASNVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(11339..>11614)
                     /locus_tag="ACHURJ_002986"
     CDS             complement(11339..>11614)
                     /locus_tag="ACHURJ_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1281849.1"
                     /translation="RVVRTVGIGAGCFCDPIQYMEYQADYYITINDSIKTWVQTQYSK
                     DSGLPMAVIGHSVAEEAGMRRLASYLDLHSGYPCIHFTGGCDYDWIE"
BASE COUNT         2818 a         2991 c         2897 g         2908 t
ORIGIN      
        1 aacaaatggt agtgtccgct attgccagta cacctcaacc agccagtcgg taaacagcgt
       61 aaacccttcc agcatgatgt tttcgcgtgg cggaattaac tcttcccact gttgctcatc
      121 acgcaggcgg gtacggtata agccaaagtt tttgccgtgg cggttggaac gcagataaaa
      181 ccgatgctgg tagtgatcaa ggctgtattc gtgatctttg cggcgcggca gaaaaacaaa
      241 cggctcggca tcggccattt ccgcgtccag caggcgaact tcactggtgg tggcgctggc
      301 caaatgaatg actacatagt gcttcgaggt cgttttatgc aggctgacgt aataggtatc
      361 gtctttttct tcgtagatca gtttatcttg cgatgctggc gtaccgatgg cgtgacgcca
      421 gacctgataa ggcagcagcg tcaccggatg cttgcgaaca tagtagaaag tccaggagtc
      481 atttgcccag acaaagctgg gttcaacgtt atccagcagt tccgggtacc agttaccagt
      541 ttccagatta cgaaaacgaa tgccgtactg gcgtcgggaa agaaaatctt ctgccagcgc
      601 cataatggtg ttatcgggcg taatcgccat tccgcccatc gaataaaact cactatgagc
      661 tgcgcgctta ttggcatcga gcaatgtttc ccactcatcc cactcttcac tgaatgccga
      721 ttgacgctgg tagatagcat attcacagcc tggttcataa atatgccgat agcggtagcc
      781 atttttgatg tagggcgcag aaacttctcg ttgcggaatg cggtcgatga tttcttttaa
      841 gatgcgatcc tgcaaggctt gttgtgaggc catcacccga tgaccgtaac tattttcttg
      901 ttgcaggtag tccaggactt ctggctgaga acgcgtatcg tcccgcagcc agtagtaatt
      961 atcgatgcgc gtatcgccat gaagcgtcat ggcgtgggga atgcgggcgg cttttggtag
     1021 catgttattg ttctttctgg ttgaaacatc ttataagggt ggcaaaactc accggggatg
     1081 caagcgaaac aggggagtca ttgcttagat gatgacaggt aatggcgcgg atatcgaatg
     1141 ttatgcagac agagaaaatc agcctgttca aatggctgtg cgattctgga tagcccgaaa
     1201 tagtcaactt caggctatcc agagagcgga attattccgc caaagtgcgt ttttgctgtt
     1261 cgagatcgcg ttcaatgccg tcacgaacat cctgggggat tttcagcgcg tcacccagtg
     1321 cattcaggta actgcgttcc ataaaatggt caatatcaat agccgcgcaa ctcaggaaat
     1381 agatttccag cgcctcttct tcattgcgga ccccggtagc caagcgttgt ggatccagcg
     1441 gttgttcgat tgcctgctca atgagtacac gcccctgctc ttccacgccg gcttcacgca
     1501 attgctggtc gattgccgca cgttctttgg catcaatatg accatcactt ttagcggcaa
     1561 agaccagcgc aaggatcaaa cgttctgtac gctcatccag cggcgtactt tgcgcgccaa
     1621 actgcggttc gtcctgatgc gccgcgcgaa ttttatcttt gtatttattc cacagcaccg
     1681 tacccgctac cgctccgccg ccaaccagta acgcgttggt gccatatttc gtaagaagtt
     1741 tacgtgctga tttatttgcg accagcagcc cagccagtcc gcctaatgcg cctggcacta
     1801 acagtttgcc caatccctga tccgcagacg aggagataga agaactgctt tgcccaagca
     1861 gggattgcag ttgatttaac cagttagcca tatttgctcc tcaataacca tttttagtgc
     1921 ctgtagcata gcggatgagg atgtcagaaa atgtatatcg agacaaaaga tgcgcaaata
     1981 agcggggcgg taggccgtta ttcaaagaaa ttcgcgccag gtaaggtatt tactgtatat
     2041 caggtgataa gggtatttta ggtgaaagta gtttgtcgtt ttgagtggca ttgtttgatg
     2101 ctgattgccc gatgcgacgc tgttgcgtct tctcaggcct acgattgtgg tgcgttctgg
     2161 ggcggacaag gcactcgcgc cgcatccgcc aacggtcaat cgaattatca gaagccatta
     2221 acgccgctga tattccgcca ttcccgcttt gcagtccacg ctgacctggt aatgaatatc
     2281 ggcactttta ccacgcacgg ttagcggtac tgaccattta tcatctttac cctgaatgtc
     2341 ctgcaaactg acccaagcca cgggatcggc ctgaccgaca attttttgat catctgccca
     2401 acgcgccacg cgattttgtt gataatcacg ttttacgctc gcggcaattc cggcagcatc
     2461 cagaccttca cactttggga aagtgaccga cttgctggtt tcattattgg cagcgaaaac
     2521 tgatgcgcag gcagaaacca acaacagccc taaaaacgcc cctctttttt tcatgttttt
     2581 ctccatagca caatgattca ggagaaagca tggtacaaat tgtcaggagc gcaagttgct
     2641 tcaggccgcg tgtgaggcat cttcctcttc ggattcagca ccgacgacct gtgggtgggg
     2701 acggtaattt gccggttttc agaatggtgc tcagcacatc tttttgttct gccagccata
     2761 gcgaaagggc ttcacgttgc tcgtcttcca tttgcactgg agaattagtc agccaggtgt
     2821 caagcagatc cgccgtatca agcattttgt cataagcatc ggcttccttt ttgctggtaa
     2881 acgacatttt ctcttcgccc tcacgaatga ctacgtattt aacttcaacc gccatttgca
     2941 gcctctcata ataactgtga ttttatacag tatatttctt ttcggttgag aaatcaaaat
     3001 caacaataaa gacacacgca aacgttttcg tttatactgc gcgcggaatt aatcagggga
     3061 tattcgttat gacgttatta ggcactgcgc tgcgtccggc agcaactcgc gtgatgttat
     3121 taggctccgg tgaactgggt aaagaagtgg caatcgagtg tcagcgtctc ggcgtagagg
     3181 tgattgccgt cgatcgctat gccgacgcac cagccatgca tgtcgcgcat cgctcccatg
     3241 tcattaatat gcttgatggt gatgcattac gccgtgtggt tgaactggaa aaaccacatt
     3301 atatcgtgcc ggagatcgaa gctattgcca ccgatatgct gatccaactt gaagaggaaa
     3361 gactgaatgt tgtcccctgc gctcgcgcaa cgaaattaac gatgaatcgc gagggtatcc
     3421 gtcgcctagc ggcagaagag ctgcagctgc ccacttccac ttatcgtttt gccgatagcg
     3481 aaagcctttt ccgcgaggcg gttgctgaca ttggctatcc ctgcattgta aaaccggtga
     3541 tgagctcttc cggcaagggg cagacgttta ttcgttctgc agagcaactt gctcaggcat
     3601 ggaagtacgc tcagcaaggc ggtcgcgccg gagcgggccg cgtaattgtt gaaggcgtcg
     3661 ttaagtttga cttcgaaatt accttgctaa ccgtcagcgc ggtggatggc gtccatttct
     3721 gtgcaccggt aggtcatcgc caggaagatg gcgactaccg tgaatcctgg caaccacagc
     3781 aaatgagccc gcttgccctt gaacgtgcgc aggagattgc ccgtaaagtg gtgctggcac
     3841 tgggcggtta tggtctgttt ggtgtcgagc tgtttgtctg tagtgatgag gtgattttca
     3901 gtgaggtctc ccctcgtcca catgataccg ggatggtgac gttaatttct caagatcttt
     3961 cagagtttgc cctgcatgta cgtgccttcc tcggacttcc ggttggcggg atccgtcagt
     4021 atggtcctgc agcttctgcc gttattctgc cacaactgac cagtcagaat gtcacgtttg
     4081 ataatgtgca gaatgccgta ggcgcagatt tgcagattcg tttatttggt aagccggaaa
     4141 ttgatggcag ccgtcgtctg ggtgttgcgc tggcgaccgc tgagagtgtt gttgacgcca
     4201 ttgaacgcgc gaagcacgcc gccggacagg taaaagtaca gggttaaacc cggcaaaaaa
     4261 acgctacaaa aatgcccgat cgtagatcgg gcgttttgac ttttacagct tagcgccttc
     4321 tacagcttca cgcgccagct tagtaatgcg gtcgtaatcg cccgcttcca gcgcatctgc
     4381 cggaaccagc caggaaccac cgatgcacag cacgcttttc agcgccaggt agtcacggta
     4441 gttagccgga gaaataccac ccgtcgggca gaaacggacc tgggagaacg gacccgcgat
     4501 cgcctgcagg gctttcacgc cgccgttagc ttcagccggg aagaatttga actctttcaa
     4561 accgtagtcc atacccagca tcagttcgga aacagtgctg atccccggaa ttagaggaat
     4621 agtcccttcg gtagcagctt tcagcagcgg ctcggtcaga cccgggctaa ttgcgaactg
     4681 tgcacccgct tcagtgactt ctgccagctg ctgtggattc agcaccgtac cggcacccac
     4741 aatcgcttca ggcacttctt tggcgatagc acggatagcg tcaactgcac actcggtacg
     4801 cagagtcact tccagaacgc gcaccccacc agcaaccaac gcttttgcca tcggcactgc
     4861 gtgttccagt ttttttacca cgataaccgg tacaaccggg ccggtggtca ggattgattc
     4921 tgcacttgtt ttccagtttt tcatcagagt tttctctcgc ctgattacaa atttgtcgtc
     4981 ttaaaaagtg atacaggttg cgccctgttc ggcaccggac agtttttcac gcaaggcgct
     5041 gaataattca cgtcctgtcc ccacgcgtga cgcgctcagg tcaggaatgt gcggttcgcg
     5101 agcagccagt tccgcttcgt ctaccagcag cgtcagttcg cctgtctgtc cattcacacg
     5161 aatgatgtcc ccgtcgcgca cttttgccag cagcccgcca tcgtaggctt ctggtgttac
     5221 gtggatagct gacggcactt tacctgaagc gccggagagt cgtccatcgg taactaacgc
     5281 aattttgaaa caccggtcca ataatacacc aagtggcggc atgagtttat gtaattctgg
     5341 cattccgttc gcttttggcc cctgatgacg gacaacaacg acacaatcgc ggtccagcaa
     5401 acccgcttca aaggccggca taacgtcatg ctggctttca aaaacaaccg ctggcgcttc
     5461 aatcacctgg ttctcaaccg gcacggcaga ggttttcata accgcacggc ccaggttacc
     5521 gcttaacact tttgtcccac catgatgaga gaaaggttgt tcgaaggaag cgatcacatt
     5581 gctgtcgagt gatttttccg ccccttcccg ccagtccagt tcaccattat tcagccatgg
     5641 ttcaagggta taacgagaca gaccaaagcc tgccaccgta ttgacatctt catgcagcag
     5701 gcctgctttg agcagttcac gcaccagaac cggtacgcca cctgccgcct ggaagtggtt
     5761 aatatcggcc ggaccgttcg ggtagagacg tgccatcagc ggtacaacat cagaaaggtc
     5821 agagaagtca tcccagttaa tctgaatacc ggccgcgcgc gccatcgcca ccaggtgcat
     5881 ggtgtggtta gtggaaccac cggtcgccag cagtgcaacg ataccgttca ccaccacttt
     5941 ctcatcgatc atcttaccga tcggcatcca ttcattacca ttaccggtca tgcgtgtaac
     6001 ctgacgcgca gcggcagcgg tcaaagcatc gcgcagcgga gaatccggat gaacaaaaga
     6061 agagcctggc gactgcatcc ccataaattc caccaccatc tggttggtgt tggcagtacc
     6121 gtagaaagta catgttcccg gcgcatggta agacgcggct tctgactcca gtaaggccat
     6181 gcggtccact ttaccttcgg cataaagctg gcgaatacgc actttttctt tatttggcaa
     6241 accgcttgcc atcggtccag acggcacaaa caccgcaggc aaatgaccaa acgacagggc
     6301 tgccatcgtc agacccggga caatcttgtc gcacacaccg aggaacagag caccatcaaa
     6361 catgttatgg gacagcccca ccgccgcaga catcgctatc acttcgcggc ttagcagcga
     6421 caattccatt ccatcctgcc cctgggtgac accatcacac atcgccggaa caccgcccgc
     6481 aacctgacca accgcattcg cttcatgcag ggctttacga atgatttctg gatagtgttc
     6541 ataaggctgg tgcgcggaga gcatgtcgtt ataggaggtg atgatggcga tattgttacg
     6601 caacatgctt ttcaaagagg ctttgtcttc tggctggcag gcagcgaaac cgtgtgccag
     6661 gttaccgcat gccaactgcg aacgatgaac ggtcgacgtt ttcgcttgtt ctatccgggc
     6721 gagataagca gagcgagtct cgcgcgaacg ttcaatgatt cgatttgtta cgcgtaacaa
     6781 ttgtggattc ataaaggctc ctgaaattga gttgtcagag caggatgatt cacaacgcgt
     6841 ttcattcaga ggatttatga gtgaaacgcc tgtaaccgga gctcataggg caaaaacgtt
     6901 tcagtcagtg taataaaaaa agcctcgtgg gtgaatccgc acgaggcctg aaagtgtaaa
     6961 aattgttcta caatctgtgc aagatcatgt taccggtaaa ataaccataa aggataagcg
     7021 cagatattac tcaaactcat tccaggaacg accatcacgg gtaatcatcg ccaccgaggc
     7081 aacgggtccc caggttccgg cctgatacgg tttcggcgca tcattgtcca tcgcccacgc
     7141 ctcagtaatg gagtctaccc atttccaggc ttcttccact tcatcacgac gtacaaacag
     7201 tgcctgaata ccacgcatgg tttccagcag cagacgttca taggcatccg ccagatgcgt
     7261 ctgattaaag gtttctgaat agctcagatc cagcttggtg atttgcaggt tatgtttgtg
     7321 gtcaaggcca ggaactttat tcagtacctg gatatccacg ccttcatcag gttgcagacg
     7381 gatagtcagt ttattctgcg gcagatcctg ccacgactct ttaaacagat tcagttcagg
     7441 tgttttgaaa tagaccacga cttcagaaca tttggtcggc agacgtttac cagtacgcag
     7501 gtagaatggc acaccggccc agcgccagtt atcaatgtcg acgcggatcg ccacgaaggt
     7561 ttctgtattg ctgctcttgt tcgcaccctc ttcttccaga tatcccggca cttttttgcc
     7621 ctgggcgaag cccgcagtat attgcccacg tacggttttt tcgcgtacgt tggagcggtc
     7681 gatgcggcgc agggacttca gtactttcac tttttcatcg cggatgctgt ctgcgctcag
     7741 gtcagatggc ggagacatcg caatcatgca gagaatttgt agcaggtggt tctgaatcat
     7801 gtcacgcatc tgaccggctt tatcaaaata gccccagcgc ccttcgatcc ccacttcttc
     7861 tgccacggta atctcaacat gatcaatggt gcgattgtcc cagttattca caaacaggga
     7921 gttagcaaaa cgcagcgcca acaggttcag caccgtttct ttaccaagat agtggtcgat
     7981 acggtaaacc tggcactcct cgaagtattc gccaacctga tcattgattt cctgcgaggt
     8041 cgccagcgac gtccccagcg gtttctccat gactacgcgt gccggtttag cattcagttt
     8101 tgcctcgcca agccctttgc aaattgcgcc aaaagtgctg ggcggcatgg caaagtagtt
     8161 aatggtgata cgattttttt gatccagcat cgcgccgaga cggttgaatg cagcagtgtc
     8221 attgacatcg agattacaaa aatccagacg tgcactcagg gtgtcccata aaccttcatc
     8281 aatggtttct ttcatgaaag tttcgagcgc ctcgcggaca actttggtat atgccgcttt
     8341 atcccagtca gcacgcccta cgccgataat ccgggtgtcc gggttgagct gaccggcttt
     8401 ttccagttga tacagggaag gcagcaattt acgacgcgca aggtcgcctt tcgcgccgaa
     8461 aatgaccagg tcacaggcct gggctgtttg cgttaccgcc atgtcattct ccttaagtta
     8521 actaacccgg tacttaagcc agggtatact tgtaattttc ttacggtgca ctgtactgct
     8581 tttacgagct tgcgaaaact gtaaacgctt atccacccgt gcgattacgg gaaaagcgcg
     8641 caaagcgcgg caaaactgat aaaaaaatca tcgttttcca ttcggtaaaa atctgacact
     8701 gatcatgtta tgaaaaaaaa taacaacttt tttatctgct tttgtcatta acacggcaca
     8761 caggcgtaat atctgacaaa actgcatttc gatttctttc agtgcggaaa tcgtcattac
     8821 ccgtgagtct ctttacatca tgaatatgct ggaaaaaatc cagtctcagc tggaacattt
     8881 gagcaaatca gagcgcaaag ttgccgaggt cattctggct tcgcccgata acgcgatcca
     8941 ttcgagtatt gctgctatgg cactggaagc caatgttagc gaaccgacgg tgaatcgttt
     9001 ctgtcgcagc atggacacgc gcggttttcc tgattttaaa cttcatctgg cacagagtct
     9061 ggcgaatggc actccctatg ttaatcgcaa tgtcaatgaa gatgacagcg ttgaatcata
     9121 cacagggaaa atatttgagt ccgcaatggc aacgcttgat catgtccgtc attcactgga
     9181 taaatctgcc atcaaccgcg ccgtcgactt gctcactcag gcaaaaaaaa tcgccttttt
     9241 cggattaggc tcttcagccg ccgttgccca cgatgcgatg aataagttct ttcgttttaa
     9301 tgttccggtg gtgtactccg atgatatcgt gctgcaacgc atgagttgta tgaattgtag
     9361 cgacggagac gtggtggtgc tgatttctca cactggaaga acaaaaaatc tggtcgagct
     9421 ggcgcagctg gctcgcgaaa acgacgcaat ggtgattgcc ctcacctctg cgggtacccc
     9481 gctcgcccgg gaagcaacgc tggcaattac cctcgacgta ccggaagata ctgacattta
     9541 tatgcccatg gtttctcgac ttgcacagct gaccgtgata gatgtgctgg cgacaggatt
     9601 tactttgcga cgcggtgcaa aattcagaga taacttgaag cgggtcaagg aagcgctgaa
     9661 ggaatcgcgt tttgataagc agttacttaa tttaagtgac gatcgctaaa aacgactgtc
     9721 actgtcctaa tcttatacga catccgaatg agattaattt atcgccatcg cggcgttatt
     9781 tcattcggat ttcatgttca agcaacacct ggttgtttca gtcaacggag tattacatgt
     9841 ccagaaggct tcgcagaaca aaaatcgtta ccacgttagg cccagcaaca gatcgcgata
     9901 ataatcttga aaaagttatc gcggcgggtg ccaacgttgt acgtatgaac ttttctcacg
     9961 gctcgcctga agatcacaaa atgcgcgcgg ataaagttcg tgagattgcc gcaaaactgg
    10021 ggcgtcatgt ggctattctg ggtgacctcc aggggcccaa aatccgtgta tccaccttta
    10081 aagaaggcaa agttttcctc aatattgggg ataaattcct gctcgacgcc aacctgggta
    10141 aaggtgaagg cgacaaagaa aaagtcggta tcgactacaa aggcctgcct gctgacgtcg
    10201 tgcctggtga catcctgctg ctggacgatg gtcgcgtcca gttaaaagta ctggaagttc
    10261 agggcatgaa agtgttcacc gaagtcaccg tcggtggtcc cctctccaac aataaaggta
    10321 tcaacaaact tggcggcggt ttgtcggctg aagcgctgac cgaaaaagac aaagcagaca
    10381 ttaagactgc ggcgttgatt ggcgtagatt acctggctgt ctccttccca cgctgtggcg
    10441 aagatctgaa ctatgcccgt cgcctggcac gcgatgcagg atgtgatgcg aaaattgttg
    10501 ccaaggttga acgtgcggaa gccgtttgca gccaggatgc aatggatgac atcatcctcg
    10561 cctctaacgt ggtaatggtt gcacgtggcg acctcggtgt ggaaattggc gacccggaac
    10621 tggtcggcat tcagaaagcg ttgatccgtc gtgcgcgtca gctaaaccga gcggtaatca
    10681 cggcgaccca gatgatggag tcaatgatta ctaacccgat gccgacgcgt gcagaagtca
    10741 tggacgtagc aaacgccgtt ctggatggta ctgacgctgt gatgctgtct gcagaaactg
    10801 ccgcagggca gtatccgtca gaaactgttg cagccatggc gcgcgtttgc ctgggtgcgg
    10861 aaaaaatccc gagcatcaac gtttctaaac accgtctgga cgttcagttc gacaatgtgg
    10921 aagaagctat tgccatgtca gcaatgtacg cagctaacca cctgaaaggc gttacggcga
    10981 ttattaccat gaccgaatcg ggtcgtaccg cgctgatgac ctcccgtatc agctctggtc
    11041 taccaatttt cgccatgtcg cgccatgaac gtacgctgaa cctgactgct ctctatcgtg
    11101 gcgttacgcc ggtgcacttt gatagcgcta atgacggcgt agcagctgcc agcgaagcgg
    11161 ttaatctgct gcgcgataaa ggttacttga tgtctggtga cctggtgatt gtcacccagg
    11221 gcgacgtgat gagtaccgtg ggttctacta ataccacgcg tattttaacg gtagagtaat
    11281 ctcacctgcc ccggccgctg atggccgggg tatttagcaa cacataaatt aggactactt
    11341 attcaatcca atcataatca cagccccctg taaaatgaat acagggatag cctgaatgga
    11401 gatctaaata gcttgcaaga cgtcgcatac ccgcttcctc tgcaacacta tgtccaataa
    11461 ctgccatcgg taagccacta tctttggagt attgtgtctg cacccatgtt ttaattgaat
    11521 cgttaatcgt aatataataa tcagcctgat attccatata ttgtattgga tcacaaaaac
    11581 agcctgcccc aatgccaaca gttctgacta ctct
//
LOCUS       DAZPEB010000132        11529 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZPEB010000132 DAZPEB010000000
VERSION     DAZPEB010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11529
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.133"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(42..110)
                     /gene="ykiE"
                     /locus_tag="ACHURJ_002987"
     CDS             complement(42..110)
                     /gene="ykiE"
                     /locus_tag="ACHURJ_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkiE"
                     /protein_id="HFR1281850.1"
                     /translation="MGWSICFWHVSVRMTHDVVCYY"
     gene            247..768
                     /gene="fecI"
                     /locus_tag="ACHURJ_002988"
     CDS             247..768
                     /gene="fecI"
                     /locus_tag="ACHURJ_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HFR1281851.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            765..1718
                     /gene="fecR"
                     /locus_tag="ACHURJ_002989"
     CDS             765..1718
                     /gene="fecR"
                     /locus_tag="ACHURJ_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric citrate uptake sigma factor regulator
                     FecR"
                     /protein_id="HFR1281852.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            1805..4129
                     /gene="fecA"
                     /locus_tag="ACHURJ_002990"
     CDS             1805..4129
                     /gene="fecA"
                     /locus_tag="ACHURJ_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent Fe(3+) dicitrate receptor FecA"
                     /protein_id="HFR1281853.1"
                     /translation="MTPLRVFRKTTPLVNTIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLSPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYLNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEIGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            4174..5076
                     /gene="fecB"
                     /locus_tag="ACHURJ_002991"
     CDS             4174..5076
                     /gene="fecB"
                     /locus_tag="ACHURJ_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HFR1281854.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAAMVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMVGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            5073..6071
                     /gene="fecC"
                     /locus_tag="ACHURJ_002992"
     CDS             5073..6071
                     /gene="fecC"
                     /locus_tag="ACHURJ_002992"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HFR1281855.1"
                     /translation="MTAIKHPVLLWGLPIAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            6068..7024
                     /gene="fecD"
                     /locus_tag="ACHURJ_002993"
     CDS             6068..7024
                     /gene="fecD"
                     /locus_tag="ACHURJ_002993"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HFR1281856.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            7025..7792
                     /gene="fecE"
                     /locus_tag="ACHURJ_002994"
     CDS             7025..7792
                     /gene="fecE"
                     /locus_tag="ACHURJ_002994"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HFR1281857.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(8351..8608)
                     /locus_tag="ACHURJ_002995"
     CDS             complement(8351..8608)
                     /locus_tag="ACHURJ_002995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1281858.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHEDIIDNNRSINWPV"
     gene            complement(8691..9178)
                     /locus_tag="ACHURJ_002996"
                     /pseudo
     CDS             complement(8691..9178)
                     /locus_tag="ACHURJ_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(9259..10767)
                     /gene="ltrA"
                     /locus_tag="ACHURJ_002997"
     CDS             complement(9259..10767)
                     /gene="ltrA"
                     /locus_tag="ACHURJ_002997"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFR1281859.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVISTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQSKTWVLFGKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
BASE COUNT         2475 a         3318 c         3113 g         2623 t
ORIGIN      
        1 cagcattacc gaaatcccca tcctttaggg cagtgaggat gtcaataata gcagaccaca
       61 tcatgagtca ttctgaccga cacatgccag aagcagatgc tccatcccat tttataccta
      121 ccttataaca cttagaaaaa caacatgtta aaaatgtcta ttggaaacaa ttttatttcc
      181 aattgtaatg ataaccattc tcatattaat atggctacgt gataattaac ttttgatgca
      241 ctccgcatgt ctgaccgcgc cactaccaca gcttccttaa cgttcgagtc gctttatggc
      301 acacatcacg gctggttgaa aagctggctg acgcgcaaac tccagtctgc ttttgatgca
      361 gatgacattg cccaggacac ttttttgcgg gtaatggtca gcgaaacgct ctcgacgatc
      421 cgcgatcctc gctccttcct gtgcactatc gccaaacgcg tgatggtgga cctgtttcgc
      481 cgaaacgcgc tggaaaaagc gtatctggag atgctggcgc ttatgccgga ggggggagcg
      541 ccttcacctg aggaacgcga aagccaactc gagaccctac aactcctcga cagcatgctg
      601 gacgggctaa acggcaaaac acgtgaagcg tttctgcttt cgcaactgga tggtctgaca
      661 tacagcgaga ttgcgcacaa actcggtgtt tccatcagct ccgtgaaaaa atacgtggcg
      721 aaagccgtcg agcactgcct gctgttccgt ctggagtatg ggttatgaat cctttgttaa
      781 ccgattcccg ccgtcaggcg ctgcgttcag cttcccactg gtatgccgtt ctaagcggcg
      841 agcgcgtcag cccacaacag gaagcgcgct ggcaacagtg gtatgaacag gatcaggata
      901 accagtgggc ctggcagcag gttgaaaacc tgcgcaacca gcttggcggt gtgcctggcg
      961 acgttgccag ccgggcgttg cacgataccc gcctcacccg ccgtcacgtg atgaaaggat
     1021 tactgctgtt gctcggcgct ggcggaggct ggcagctctg gcagtcggaa accggcgaag
     1081 gtctgcgggc agattaccgc accgccaaag gcacagtcag ccgtcagcaa ctggaagatg
     1141 gctccctgct cacgctgaat acccaaagcg cggcggatgt gcgttttgat gcgcatcaac
     1201 gcaccgtccg gctctggtac ggtgaaatcg ccattactac cgcgaaagat gccctgcaac
     1261 gccccttccg tgtcctgacc cgtcagggcc agctcactgc tttagggaca gaatttaccg
     1321 tccgccagca ggataatttc acgcagcttg acgtgcagca gcacgctgtg gaagtgcttc
     1381 tcgccagtgc ccccgcgcaa aaacgcatcg tgaacgctgg tgaaagcctg cagttcagcg
     1441 cctctgagtt tggcgcagtg aaaccgctgg atgacgagag tacaagctgg acgaaggaca
     1501 tcctgagctt cagcgataaa ccgctgggtg aggtgatagc cacgctaacc cgttaccgca
     1561 acggcgtgct gcgctgcgat cccgccgttg ccgggctgcg cctgagcggg acgttcccgc
     1621 tgaaaaatac cgatgcgatc ctgaacgtta tcgcgcaaac gcttcccgtt aaaattcagt
     1681 ctattacgcg gtactggata aacatttcac cactgtaagg aaaataattc ttatttcgat
     1741 tgtccttttt acccttctcg ttcgactcat agctgaacac aacaaaaatg atgatgggga
     1801 aggtatgacg ccgttacgcg tttttcgtaa aacaacacct ttggttaaca ccattcgcct
     1861 gagcctgctg ccgctggccg gtctctcgtt ttccgctttt gctgcacagg ttaatatcgc
     1921 accgggatcg ctcgataaag cgctcaatca gtatgccgca cacagcggat ttaccctctc
     1981 ggttgacgcc agcctgacgc gcggcaagca gagcaacggc ctgcacggcg attacgacgt
     2041 cgagagcggc ctgcaacaac tgctggacgg cagcggactg caggtaaaac cgctgggaaa
     2101 taacagctgg acgctggagc ccgcgcccgc accaaaagaa gatgccctga ccgtggtcgg
     2161 cgactggctg ggtgatgcgc gtgaaaacga cgtatttgaa catgctggcg cgcgtgacgt
     2221 gatccgccgt gaggatttcg ccaaaaccgg cgcaaccacc atgcgtgagg tacttaaccg
     2281 catccctggc gtcagcgcgc cggaaaacaa cggcaccggc agccacgacc tggcgatgaa
     2341 ctttggcatc cggggcctga gcccgcgcct cgccagccgc tcgaccgtcc tgatggacgg
     2401 catccccgtc cccttcgccc cttacggtca gccgcagctt tcactggctc ccgtttcgct
     2461 cggcaacatg gatgccattg acgtggtacg cggtggtggt gcggtgcgtt acggaccgca
     2521 gagcgtgggc ggcgtggtga actttgttac ccgtgccatt ccgcaggact ttggtatcga
     2581 ggcgggcgtg gaaggtcagc tcagcccaac ctcttcacaa aacaacccga aagagacgca
     2641 caacctgatg gtgggcggca cagcggacaa cggttttggc accgcgctgc tctactccgg
     2701 cacgcgcggc agtgactggc gcgagcacag cgccacccgc atcgacgacc tgatgctgaa
     2761 aagcaaatat gcgccggatg aggtgcacac cttcaacagc ctgctgcaat attacgacgg
     2821 tgaagccgac atgcccggtg gcctgtctcg cgcggattac gacgccgatc gctggcaatc
     2881 cacccgcccg tatgaccgct tctggggtcg tcgcaagctg gcgagcctgg gctaccagtt
     2941 ccagccagac agccagcata aattcaacat tcaggggttc tacacccaaa ccctgcgcag
     3001 cggctacctg gagcaaggca aacgcatcac cctctcgccg cgtaactact gggtgcgcgg
     3061 tattgagcca cgctacagcc agatctttat gatcggccct tccgcgcacg aagtgggcgt
     3121 gggctatcgc tatttgaatg aatcaacgca tgaaatgcgt tactacaccg ccaccagcag
     3181 cgggcagttg ccgtccggct caagccctta cgaccgcgat acgcgttccg gcaccgaggc
     3241 gcacgcctgg tatctggatg acaaaatcga catcggcaac tggaccatca cgccgggtat
     3301 gcgtttcgaa catatcgagt cataccagaa caacgccatc acaggcacgc acgaagaagt
     3361 gagctataac gcaccgcttc cggcgttgaa cgtgctctat cacctgactg acagctggaa
     3421 tctttatgca aacactgaag gctcgttcgg caccgtacag tacagccaga ttggcaaggc
     3481 tgtgcaaagc ggcaatgtgg aaccggaaaa agcgcgaacc tgggaactcg gtacccgcta
     3541 cgacgacggc gcgctgacgg cggaaatagg gctgttcctg attaacttta acaatcagta
     3601 cgactccaac cagaccaacg acaccgtcac tgcacgtggc aaaacgcgcc ataccgggct
     3661 ggaaacgcag gcacgttacg atctgggtac gctaacgcca acgcttgata acgtttccat
     3721 ctacgccagc tatgcgtatg tgaacgcgga aatccgcgag aaaggcgaca cctacggcaa
     3781 tctggtacca ttctccccga aacataaagg cacgctgggc gtggactaca agccaggaaa
     3841 ctggacgttc aatctgaaca gcgatttcca gtccagccag tttgcggata acgccaatac
     3901 ggtgaaagag agcgccgacg gcagtaccgg ccgcattccc ggcttcatgc tctggggcgc
     3961 acgcgtggcg tatgactttg gcccgcagat ggcagatctg aacctggcat tcggtgtgaa
     4021 aaacatcttc gaccaggact acttcatccg ctcttatgac gacaacaaca aaggcatcta
     4081 tgcaggccag ccgcgcacgc tgtatatgca ggggtcgttg aagttctgat gtgttaacgc
     4141 ccggcttgcc gggcttttag ctggaatgtg attatgttgg catttatccg ttttcttttt
     4201 gcaggcctgc tgctggtgat cagccacgcc tttgccgcca tggttcagga cgaacacggc
     4261 acgtttacac tcgaaaaaac gccacaacgg attgtggtgc tggaactctc gttcgccgat
     4321 gcgctggccg ccgtggacgt cagcccgatc ggtattgccg acgataacga tgcaaaacgc
     4381 atcctgcccg aagtgcgtgc gcacctgaaa ccgtggcagt ccgtcggaac gcgcgcgcag
     4441 ccgagcctgg aagccattgc cgctctgaaa ccagacctga tcattgccga cagcagtcgc
     4501 catgcggggg tttacatcgc cttgcagcaa atcgcgccgg tactgctgct taagtcccgc
     4561 aacgaaacct acgctgaaaa tttgcaatct gcggctatca tcggcgaaat ggtgggtaaa
     4621 aagcgagaga tgcaggcacg tctggaacaa cataaagaga ggatggcgca gtgggccagc
     4681 cagcttccca aagggacacg cgtggccttt ggcacatcac gcgaacagca attcaacctg
     4741 catactcagg agacctggac cggcagcgtg ctggcctctc tggggctgaa cgttcccgct
     4801 gcgatggtgg gtgcgtccat gccgtccatc ggcctggagc aactgctggc ggtcaatcct
     4861 gcctggctgc tggttgccca ctatcgcgaa gagagcattg ttaaacgctg gcaacaagat
     4921 ccgctctggc agatgttaac cgccgcgcag aagcagcagg ttgcttcggt cgacagtaac
     4981 acctgggcgc ggatgcgcgg tatttttgct gcagagcgta ttgccgctga cacggtaaaa
     5041 atcttccacc atcagccgct taccgttgtg aaatgaccgc gataaaacac ccggtgctgc
     5101 tgtgggggct tcccattgca gcacttatta ttattttctg gctgagtctg ttttgctact
     5161 cggccattcc tgtttccgga gcagatgcaa cccgcgccct gctgcctgga cacacgccaa
     5221 cgctaccaga agcgctggtg caaaaccttc gtttgccacg aagcctggtc gccgttctga
     5281 tcggcgcaag cctggcgctc gcgggcacgc tgctgcaaac cctgacccac aacccaatgg
     5341 cctctccttc actgctcggc attaacagcg gcgcggcgct ggctatggcg cttaccagcg
     5401 cgctaagtcc gacgccgatt gcaggctatt ctctgtcgtt catcgcggca tgcgggggcg
     5461 gcgtgagctg gctgctggtc atgaccgcag gaggcggatt tcgtcatacc catgacagaa
     5521 acaaactgat cctcgcgggt atcgcgctgt cggccttttg tatgggcctt acccgcatca
     5581 ccctgctgct ggccgaagat catgcttacg gcatctttta ctggctggca ggcggagtgt
     5641 cccacgcccg ctggcaggat gtctggcagc tcttgccggt ggtggtcact gcagtccctg
     5701 tcgtgttgct gctggcgaat caactgaacc tgctcaacct cagcgacagc accgcccata
     5761 cgctgggagt gaacctgacg aggctacgtt tggtcatcaa tatgttagtg ctgcttctgg
     5821 ttggtgcgtg cgtcagcgtg gcaggtccgg tggcgtttat cggtctgctg gtgccacatc
     5881 tggcgcgctt ctgggcaggc ttcgatcagc gcaacgtact gccagtgagc atgctgctgg
     5941 gggccacgct gatgctgctg gcagatgtac tcgcacgcgc gctggccttc cccggagatc
     6001 tgcccgcagg cgcagtgctg gcgctgattg gcagcccttg ctttgtctgg cttgtgagga
     6061 ggcgaggatg aaaattgcgc tggttatttt catcaccctt gccctggcgg gctgtgcgct
     6121 gttatcactc catatgggag tgatccccgt gccgtggcgc gcgctgctga ccgactggca
     6181 ggccggacgc gagcattatt atgtattgat ggagtaccga ctgccgcgct tgctgctggc
     6241 actgtttgtc ggtgcagccc tcgccgtggc gggcgtgctg atacagggga ttgtgcgcaa
     6301 ccctctggca tcaccggata ttctcggcgt taaccatgcc gccagcctgg cctctgtggg
     6361 ggctctactt cttatgccgt cactgcccgt gatggtgctg ccgctgctgg cctttgcggg
     6421 cggcatggcg gggttgatat tactgaagat gctggcaaag acccaccagc cgatgaagct
     6481 ggcgctcacc ggcgtggcgc tttctgcatg ctgggccagc ctgacggatt atctgatgct
     6541 ctcgcgccca caggatgtga acaacgccct gctgtggctg accggcagct tatggggccg
     6601 tgactggagc tttgtgaaga ttgccatccc gctgatgatt ttatttctgc cgctgagcct
     6661 gagtttttgc cgcgatctcg acctccttgc actcggcgat gcgcgcgcca ccacgctcgg
     6721 tgtgtcggtg ccccataccc gattctgggc tttgttacta gctgtcgcca tgacatctac
     6781 cggcgtggcc gcctgcggcc cgattagctt tattggtctc gtggtgccgc atatgatgcg
     6841 tagcatcacc ggtggacgtc accgcagact gctgcctgtt tcagccctga caggtgcgtt
     6901 gctgttggtg gttgccgatc tgctggcgag aattattcat cccccactgg agctcccggt
     6961 tggcgtgctg accgccatta tcggtgcgcc gtggtttgtc tggttgcttg tgagaatgcg
     7021 ataaatgact ttacgaactg aaaatctgac ggtcagttac gggacagaca aggtacttaa
     7081 cgacgtttca ctctcactgc caacggggaa gatcaccgcc ctgatcggtc ctaacggttg
     7141 cgggaaatcg acgctgttaa actgtttttc gcggctttta atgccgcagt ctggcaccgt
     7201 atttctcggc gataatccca taaatatgct ctcatcgcgc cagttggccc gcaggctttc
     7261 gctgctgcct cagcaccatt taacgccaga ggggatcaca gtccaggagc tggtttcgta
     7321 tggtcgtaat ccctggctgt cactctgggg gcgtctctcc gctgaagaca atgcacgagt
     7381 taatgtcgcc atgaaccaga cccggatcaa tcatcttgcc gttcgtcggt taaccgagct
     7441 ttccggcggt cagcgccagc gcgcatttct ggcgatggtc ctggcccaga atacgcccgt
     7501 tgtattactt gatgagccaa ccacctatct tgatatcaat caccaggtgg acctgatgcg
     7561 gttgatgggc gaactccgga ctcaggggaa aacggtggtc gctgtgctgc acgaccttaa
     7621 tcaggctagc cggtactgcg atcaactggt ggtaatggca aacggacatg ttatggcgca
     7681 aggcacacca gaagaggtga tgaccccagg attgctgaga acagtattca gcgtggaagc
     7741 ggaaatacac cccgagccgg tatctggcag gccgatgtgc ctaatgaggt agattgcaca
     7801 ggccgtaaga accaaaccac gactgaatga aactggactg gcgccagcaa gcctgttcag
     7861 actggggctg aacttttccg gactctgaaa gattaccaat actcatcgtc catccgcttg
     7921 ctttaggctg acaggttcat aattaacgca aaccagagct gtacaggctt gggcgcggct
     7981 ttcaaaccaa tcgtgatcac ggcaatcaat tttgaactct gcttaacgga catttctgta
     8041 taacccttac ggcaacgaaa aacgcgaagt taaaatttta gaaacccaaa aacgtgacat
     8101 gactaagttt agatttcagg ggggggagat caaaaaattt cgctctgtgc cagagcggac
     8161 attcacggag ctggttcatt accaatgagg ttgggctttt gaggataaat caatgatcag
     8221 acgccaacgt aaatcaaaag caaccctgga acggaattgc taatccagtt tctgaccatc
     8281 gatttttcta aaaagtgtgc gtttgctact acttaggtag gtgcagcttt cttaatcacc
     8341 ggcagccacg ttatacaggc cagttgatgg atcgattgtt atcaatgata tcttcatgag
     8401 tcggtgtctc acccagctta ccgaagctgg cataaagtga aggcagacgg gcccgtcctt
     8461 ctcccttttt cgccagaggg aaagcgcgaa gcatggtggc agcctcccag tcacaacaat
     8521 aggatggtgt gcacggctgc tgacgccatg attcagcgat agagccggaa aatacggggt
     8581 caaagccggt atcattaacc agagtcatcg tgacctgtac tgcggctgga tgatctcctg
     8641 ctactgcaac tgctaggcga ccgcggctcc cttcaggtag tctgttgtgg tcaactaaaa
     8701 ctggcctccg cgttagagtt tttccagtat cggttttctg attcgtttgg tggtaaccca
     8761 ccattatatt cgtgcggtct tagtgcgctg taatatccaa cgatatagtc cgttattgcg
     8821 tgagctgcat cgctgaagct tacatagccc gtcgctggca cccattcgtt cttcagactc
     8881 ctgaagaagc gctccattgg gctgttatcc cagcagtttc cacgccgact catactctgc
     8941 ctgatccggt atcgccacag taactgccgg aactgcctgc tcgtataatg actgccttga
     9001 tcgcctggaa catcaccccg acgggcttac cacgggtttc ccatgccatt tccagtgctt
     9061 tcatggtaag cctgctgtcc ggcgagaacg acatggccca gcccactggt tttcttgcga
     9121 acaggtcgag aacaacggcg aggtacgccc agcgcttacc cgtccagagt agagtaagag
     9181 gcagggcgta gtcggactat ttccctgcct ctcctccccg aaccggacgt gcacctttca
     9241 gcgcatccgg ctctccattt aaatgctggc gaacgccatt gccacttctg taaagcgcga
     9301 tgtatacgtg tttctggtct ccgtcctcag atagggatta ccttcgggta gccgccagcg
     9361 gaacagcttc ttgccttgcc ccaccaaccg gtacagtatt tcgccgctga gcttgccgtg
     9421 attggtttta ccaaataaaa cccacgtttt gctctgaccc ggtttcggtg atttacacca
     9481 ccacctcatc agggaagcga tacctgtacg gtatttgcgg gccagccagt gagccagctt
     9541 ccagaacacg acacggtcga tataactgaa gactttggcc ttaaaatcaa cgaactgata
     9601 gaacatggcc cagcctttca gttttcggtt gagttgttca gccatatcga ctttgctttc
     9661 actgtagttg cctgataaca gtgctgtcag cgatgcggcg aagtttctgg ctttctcctg
     9721 cgggatcgtt gagatcactc gcatctcgcc ataacgactg cgtttgcgaa tgatcctgtg
     9781 ccccagaaag ataaagccgt cattaacatg ggtgatttta gtcttatcca tgttcagcct
     9841 gagtttcaga ctgccttcga gcacaccccg acactcctcc ctgatggctt ccgcctgtgc
     9901 tttggtgcct ttgacgatga ggacaaaatc atcggcatag cggcagtacg ccaccgcggg
     9961 tttccactgc cagttttctc tgaccgccgt acttcggccc cgttggatac tgttattcca
    10021 gtaccaccga tcttttctgg ctttcccgct caggtagcgc tcatgcaggt attgatcgaa
    10081 ctcattcagc atgatgttcg ataatagcgg cgatataaca ccgccctgtg gcacaccttc
    10141 actggccgcc cgaaagagac cgacatcgat atgtcccgcc ttgatggttt tccacagcag
    10201 agtcatgaaa cgtgcgtcac tgatcctgcg gcgtacagcc ttcatcagca gtcgatgatg
    10261 tacggtgtcg aagtaactgg acaggtcgcc ttcaatcacc cagcgtcccc gggtttcacc
    10321 gcagtctgtg agctgtaatt tcaccgtgcg gatcgcgtgg tggacactgc gctcaggccg
    10381 gaagccatat gagagcgtat gaaaatcact ctcccatatc ggctccatcg ccatcagcat
    10441 ggcccgctga acaatacgat cccgcaacgc ggggataccc agtggtcgca gtttgccgtt
    10501 gcttttaggg atgtaaaccc gtcttgcggg caagggctgg tagtggcctg agagtaattc
    10561 atccctgagg atttgcagct caacagccag tctggcctgt agcattgttt tgttcacgcc
    10621 atcaacgccg ggggtatggg ccccctttga tgaaagcgtg atccgcgccg cttcagccag
    10681 ccattctggt tgtgttatta gacgcagcag ccgttgaatc cgtagggacg gatcggtggc
    10741 tgcccatgtg gcaagcttgc gttgcatttc gctgattatc aaaggtcttc acctcgttag
    10801 gtcagttaat tcacgtcgca aacacattca aactgcttcc cttcgccatg taatgggctt
    10861 tccccatcgc ggactactac ggaagctccg ccagccagcg cgtcatcgga gccatgcccc
    10921 cttaacatcc gtcgctgacc ttccccggtt tacctgcctg gactcaggca tactgaggag
    10981 gctgcccgtc gcactcttta tccttgcttg ccgcaagttg gcagaagtca gcaacgcaag
    11041 cgtgatagac gctgctgccc cggtgtttcg catacatgtc aaaacacctt cgaccggcag
    11101 tgcttacgta tcactgccag ttcctcctgc acggcctgtc agatcacgta ggccgtggtg
    11161 acgttttcaa cccacagagg cggattaacg ggttcatgtt cttcagcctt tcagtactta
    11221 accttgagga tcatctcggc ttagtgatct cgcctcaatc cccgttgtca gcgggttaca
    11281 tcaccctgcg ggcatgccgc aggtcactgc cgctcaggtt ctccaccgtc acacccggtg
    11341 ggattgttgg gtttctcatc gtgagttacc ggttcaatat tccagacaga ctcgcggttc
    11401 atttaagcat ccatgcccgc cctgaactcc gggcacacta taggtcacat caccgcacca
    11461 cacctgattt ggttccgtta cggcgaactg tcgctcaaga tgattcggga tagcaacgtg
    11521 ctcatgacc
//
LOCUS       DAZPEB010000133        11405 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZPEB010000133 DAZPEB010000000
VERSION     DAZPEB010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11405
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.134"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..921
                     /gene="gap"
                     /locus_tag="ACHURJ_002998"
     CDS             <1..921
                     /gene="gap"
                     /locus_tag="ACHURJ_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFR1281860.1"
                     /translation="DVVAINDLTSPKILAYLLKHDSNYGPFPWSVDFTEDSLIVDGKS
                     IAVYAEKEAKNIPWKAKGAEIIVECTGFYTSAEKSQAHLDAGAKKVLISAPAGEMKTI
                     VYNVNDDTLDGNDTIVSVASCTTNCLAPMAKALHDSFGIEVGTMTTIHAYTGTQSLVD
                     GPRGKDLRASRAAAENIIPHTTGAAKAIGLEIPELSGKLKGHAQRVPVKTGSVTELVS
                     ILGKKATAEEVNNALKQATTNNESFGYTDEEIVSSDIIGSHFGSVFDATQTEITAVGD
                     LQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(963..2403)
                     /gene="aldA"
                     /locus_tag="ACHURJ_002999"
                     /pseudo
     CDS             complement(963..2403)
                     /gene="aldA"
                     /locus_tag="ACHURJ_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
     gene            complement(2558..3400)
                     /gene="ydcF"
                     /locus_tag="ACHURJ_003000"
     CDS             complement(2558..3400)
                     /gene="ydcF"
                     /locus_tag="ACHURJ_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFR1281861.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALFNQAVERVHTAIVVQDPTMQRSTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLRQKLPVCEPLV
                     CKRA"
     gene            complement(3672..7574)
                     /gene="hrpA"
                     /locus_tag="ACHURJ_003001"
     CDS             complement(3672..7574)
                     /gene="hrpA"
                     /locus_tag="ACHURJ_003001"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFR1281862.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSGTIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVLGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            7775..8380
                     /gene="azoR"
                     /locus_tag="ACHURJ_003002"
     CDS             7775..8380
                     /gene="azoR"
                     /locus_tag="ACHURJ_003002"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFR1281863.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(8431..9747)
                     /locus_tag="ACHURJ_003003"
     CDS             complement(8431..9747)
                     /locus_tag="ACHURJ_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HFR1281864.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWPFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAER
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGAGSCIVLMELMVMIQLWWPVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKITAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGHCKTVDEAISYIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(9737..11405)
                     /locus_tag="ACHURJ_003004"
                     /pseudo
     CDS             complement(9737..11405)
                     /locus_tag="ACHURJ_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415928.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
BASE COUNT         2844 a         3097 c         2711 g         2753 t
ORIGIN      
        1 gacgttgtcg ctattaatga tctcacttcc ccaaaaattc tcgcctacct gctgaaacat
       61 gattcaaact acggaccatt cccctggagc gttgatttta cggaagattc acttatcgtt
      121 gatgggaaaa gtatcgcggt ttacgccgaa aaagaggcta aaaatattcc gtggaaagcg
      181 aaaggtgcag aaatcattgt cgaatgtact ggcttttata cctccgccga gaaatcgcag
      241 gcgcatcttg atgctggtgc gaagaaggtg ttgatttccg cccctgccgg tgaaatgaaa
      301 actatcgttt ataacgtcaa tgacgacact ctggatggca acgacaccat tgtttccgtg
      361 gcgtcatgca ccactaactg tcttgcgccg atggccaaag ccttgcatga cagtttcggg
      421 atagaagtcg gcacgatgac gaccattcat gcctatactg gcacccagtc actggtggat
      481 ggcccacgtg gtaaagattt acgtgcttca cgcgcagcgg cagaaaatat cattccccac
      541 actacggggg cggcaaaagc cattggtctg gagatcccgg aactgagcgg caaactgaaa
      601 ggtcatgcgc aacgcgtgcc ggtgaaaaca ggttcggtca ctgaactggt atcgattctc
      661 ggaaaaaaag cgactgccga agaggtgaat aacgcactta aacaagcaac caccaataac
      721 gagtcatttg gttataccga tgaagaaata gtctcttccg atatcattgg cagccatttc
      781 ggttcggtgt ttgatgccac gcaaacggaa attaccgccg tgggcgattt acaactggtg
      841 aaaacggtcg cctggtacga taacgaatat ggcttcgtca cgcagcttat tcgcaccctc
      901 gaaaaattcg ctaaactctg acgcgcacag gcggaggaaa aaacctccgc ctcttttact
      961 cattacgact gtaaataaac cacctgggtc tgcagatatt catgcaggcc atgtttacca
     1021 tctgcgccgc caataccgga tttacgccat ccggcgtgga agccttgcat agcttcgaag
     1081 ttttcacggt tgatgtaagt ttcaccaaac ttcagccctt taatggcttt catcgcgacg
     1141 ttcagatttt gggtatagat ttgatgaggt caggccgtaa tcactgtcat tagccattga
     1201 gatagcctct tccagcgtgt caaatgcgac caccggcaga accgggccaa aggtttcctc
     1261 atgcataatc gacatttcct ggcgaacatc cagcagcaat gtcggcggat aataatatcc
     1321 tttcccctct accgctttgc cacccaacgc cactctcgcc ccttcttcta ctgcgcgcgc
     1381 cactttttgc tcaacccttt ccaacgccgc ggcgttaatc aacggcccca tcgcaatgtc
     1441 gttgcgttca gcggggttac caaattgaac cgcctgcatc gcttcaccca gccgattgac
     1501 gaactgatca taaatgcctt tctgtacata aacacgttct gcacagttac acacttgccc
     1561 actattaatg acgcgtgaat caacgatggc tttgactgcc agttcaagat cggcatcgtc
     1621 cattacgata gctggtgctt taccccccaa ttccagacac actttggtga tgtttttcgc
     1681 cgcagtcgcc atgatcttct cacctgcaga gacgctgcct gtcatactga ccattgcgac
     1741 ctttgggtta cccgccagtt cttgcccaac ggtttcacca cgccccagta caaggttaaa
     1801 cacgccgcgc ggaaggccta tttcatcgac gattttggcg aatgcaatcg cattgtttgg
     1861 cgtaaattca ctaggtttaa tgacgatggt attaccggtc aaaagagcgg gagccatttt
     1921 gcgggcaatg aggaagaacg ggaagttcca cggcagaatg ccggtagtca caccaagcgc
     1981 acgtttaaac aaaagaatat tttctcctgg acgatcgctt tgaataatct cgccctcgta
     2041 acgccgtgcc cactccgcca tgtaatcgat atagtcggca gtaaaagcca cttcgacttc
     2101 agccagctgc tggatcttgc ccccttcttc aacaatcagc gcactgattt cactggcgcg
     2161 ttcgcggatc ccggcggaga ttttgcgcaa ccaactggcg cgttcaatag caggcaacgc
     2221 ttcccattct ggttgtgcac gttctgctgc atcgattgcc ttacgggcat cctcggcctg
     2281 accatcgggt atgcgggaaa tgacagcctc tgtagcaggg tttaccacat caatccatgc
     2341 gtctccacgc caggtaacaa actgtccatc gatatacata ggatgttgaa cgggtactga
     2401 catgggcgac tcctgtgatt tatatgtttg ttcttggtaa atacattgtt aattaacatc
     2461 aatcatcata gagacggcat cgtgaaagga caattctgtg aagggcttca taaaaacaag
     2521 tgtaaaagga ataaaaggca gaaataaaag tcaggagtca tgcccgtttg caaacaagtg
     2581 gttcacaaac gggcaatttt tgacgtaacg agcgactttc catcgcctcg atgagcaccg
     2641 catcgtgttt aagcgtttgc catgcatgga tgacttctgc cggaaaatca acgtgaacga
     2701 taaaatctcg cccgcgggga ccgtagccat cgctatcatc gcgtaaacgc ggcagctcgc
     2761 cagtgagtag tgagagataa cgctcaaccg gccataatcc ttgtaactga ttaataaaga
     2821 ttacactgtc tgcgttattt cctaactgag gaacgaatcc gggataactt aaccagcgtg
     2881 gtgcatcggg attgtcccca gtcatacggc ggaacgtcgc catcgtgctc cgctgcatgg
     2941 tggggtcctg aacaacgata gccgtatgaa ctcgttctac ggcctgatta aatagcgcga
     3001 tgctaaagcg tgcgttttca ccgcagtttg ttgactggtc ttcaatccag attttttcat
     3061 gcggaatgtg ccagaactga tgagcgatat ccgccaggat ggtcgcttct gctctgccag
     3121 tggtgcggat agtgttgtag tgcggatgct gtgcgatggc gctatacaaa aaagttgtcg
     3181 agtgaccgat accaccacta atcagtaaag gaatttgctg atcgcgagca atcttacatg
     3241 ccgcgtcgat agtcggcata accgcattgc ctgcaaggat cacgcaatcg gcctgatacg
     3301 gcacctcacc ggagaaatca tcctgcgcca gccactgtcc gataacattt atggcatcta
     3361 tagttgccgg cgaaagcgtc ggaaacgggg taatgttcat ggttcctcct tatcgttccc
     3421 ttaagaatac tgcgtgagat aatagtgaga agctgagcac tctgaaagga ggaagtcgta
     3481 tgtccttaac cgccactccg caagcctgac atatctttta aggaaatgct gtagataaac
     3541 attattcatc ttgcagcaga gaattgtatt tttctgaatt cctgaatttt tacccttacc
     3601 gttttagaat taataaaaaa cccggcgcta aatgcttacc gggttttaga tttattaggc
     3661 aaatagcagg gttaaccgct aatctgctcc atcgcctgca aaatacgctt atctgaaatc
     3721 ggataaggcg taccaagttg ttgagcgaag taactaacgc gcagctcttc tatcatccaa
     3781 cggatctctt tcacgtcttc atcctcacga cgtgcaggcg gcagtttgtt gatccattgc
     3841 tgccacgcct gctggacgtt ttcgactttc agcatctgtg cacgatcgcg atgtggatca
     3901 accgccagtt tttccagtcg tttttcaatc gcctgcaaat atcgcagcgt gtcgcccagc
     3961 cgtttgaagc cgttaccagt gacaaaaccg cgatatacca acccgcccat ctgcgcttta
     4021 atgtcagaaa gccccagtgc catggtcata tccacccgcc ctttcagacg tttgttgata
     4081 ttgaacactg ccgtaagaat ttgctcgacc tgcttcgcaa tatctaccac cgtgtcgttc
     4141 agttcggcac gcactttctc atgcagcgca gcaaagcctt cttccgtcca gaccgggcca
     4201 ccattggcgt cgatcagttg atccacaccg caggagatac agtcgtcgat cagctccagc
     4261 actttgccat acgggttgaa gtacagtccc agcttggctt tgttcggtaa cttttcatgt
     4321 aaatacttga tcggcgaggg aatatttagc agcagtagac ggcgaagacc attccacatt
     4381 gcctgttttt gctccagcgg gttatcaaac agtttgatcg ccacgctgtc gcgttcatcc
     4441 accagcgccg gccacgcctt cactttatag ttgccacgct tctgttcgta actttccggc
     4501 agctgaccaa aactccagat atgtaagccg ctctgctcga taccgtcatc cgccaccgcg
     4561 gacagcgttt cctgcacttt gcctttcagc gcatctttca gatcttgtag cgagcgccct
     4621 tcttttagct tcttgttttt gtcatccacc acgcggaagg tgattttcag gtgatcgggc
     4681 acctgatccc agtgccagtc ttcgcggtca acggtaacgc cggtcatccg ccgtaactcg
     4741 cgctcaaggc tgtcgagcaa cggtaactcc agcggtgtga cgcggcctaa aaacgcttcg
     4801 gcgtagtttg gtgcaggcac aaaattacgg cgtaccggtt tcggcaacga tttaatcaga
     4861 gcaatcacca attcgcggcg cagaccgggg atctgccatt caaacccgct ttcctcaacc
     4921 tggttaagta acggcagcgg aatatgtacg gtcacaccgt ccgcgtctgc gcctggctca
     4981 aactgatagc tcaaacgcag cttgagattg ccctgatgcc agaagttcgg gtaatccagc
     5041 ttgctgattt tttccgcccc ttctttgatc aacatgctct tttcaaagtt gagcaaatca
     5101 ggcgtttcgc ggctgacttt tttccaccag ctatcgaagt gacgagcgga gattacatcg
     5161 tggctgatgc gctggtcgta gaactcaaac agcgtttcgt catcaaccag aatatcgcgg
     5221 cgacgtgatt tgtgttccag ctcttccact tcggcccgca gtttcaggtt ttcacggaag
     5281 aatgcgtgac gcgtctgcca gtcaccttcc accagcgcat ggcgaataaa gagttcacga
     5341 cataacgccg gatcgatctg gctgtagttg accttgcgcg cggcaacaat cggcaaacca
     5401 taaacagtga ctttttccgt tgccatcacc gcgccctgcg cccgttccca gtgcggttcg
     5461 ctgtaggtac gtttaatcaa atgctgagcg acaggctcca cccattccgg gtcgatacgc
     5521 gcagcaatgc gcccccacag gcggctggtt tctaccagtt ccgccaccat tacccatttc
     5581 ggcggttttt tgaataaacc agaaccgggg aagatggaga aacgcgcgtt acgtgcgccg
     5641 gtatattctt gtttatcggc atctttcatg ccgatatggg aaagtaaacc cgtcagcaac
     5701 gcaatgtgaa tttcgcgata ctccgccggt tcgctgttaa ccggaatgcc aagttctttc
     5761 accacctgac gcaactgggt gtagatatcc tgccattcgc gcacgcgcag atagttgaga
     5821 taatcggtac gacacaggcg acggaaggcg tttgaagaaa gcgccttttg ctgctcgcca
     5881 agataattcc acagatttac aaacgcgagg aagtcggatt ctttgtcgtg gaagcgacga
     5941 tgtttttcgt ccgatgcctg ctgtttgtcc atcggccgct cacgcgggtc ctgaatggag
     6001 agcgcggacg tgataatcat cgcctcacgc acgcagccat gtttctgcgc ttccagcacc
     6061 atacgcgcca gacgaggatc gaccggcagt tgcgagagct ggcgaccgag cggcgtcagt
     6121 ttataggcgc tggcctgttc atccgtggtg atcgcgccca gttcttcgag cagacgcacg
     6181 ccatcctgga tattgcgttt atccggtgct tcgacaaacg ggaacgcagc gatatcgccc
     6241 agccccagcg cggtcatctg caaaataacc gaggccaggt tggtacgcag aatctccgga
     6301 tcggtaaact ccgggcgcga gaggaaatcg tcttcggaat agagacgaat acagatccct
     6361 tcggacacac gaccacaacg gcctttacgc tgattggcag acgcctgtga aatgggttca
     6421 atcggcaaac gctgcacttt ggtgcgatag ctgtagcggc tgatacgcgc cgtaccgggg
     6481 tcgataacgt atttaatccc cagtacagtc agcgaggttt ccgcgacgtt ggtcgccagc
     6541 acaatgcgtc gcccgctgtg cgactggaac acgcggttct gttcgctatt cgaaagccgc
     6601 gcataaagcg gcaagatttc ggtatggcgt aagttcagct tgttcagcgc atcggcggta
     6661 tcgcgaattt cccgctcgcc gctcataaag atcagaatgt cgccagggct ttcctggctc
     6721 agttcgtcta cggcgtcaaa aatcgcctgc aactgatcgc gctcggtgtc atcggcttct
     6781 tcaacaatcg ggcgatagcg cacttccacc ggataggtcc gaccggagac ttcgataatc
     6841 ggcgcattat taaagtggcg agaaaagcgt tccgggtcga tagtcccgga agtgataatg
     6901 atttttaggt caggacgccg cggcagcaac tctttcaaat agccgagcag aaaatcgata
     6961 ttcaggctgc gttcgtgtgc ttcgtcaata atgatggtgt cgtactgcat cagcaggcgg
     7021 tcttgctgga tctccgccag caggataccg tcggtcatca gcttgaccat cgtgttatca
     7081 cttacgtgat cgctgaaacg cactttgtaa ccgatgcaac cgcctggctc cgttttcagc
     7141 tcttccgcaa tacggttcgc caccgttctt gccgccagac gacgcggctg ggtatggccg
     7201 atcagtcctt taatcccgcg ccccagctcc atacagattt tcggtagctg ggtcgtttta
     7261 ccggaaccgg tttccccggc gacgatcacc acctggtgat cacgaatcgc ttcgagaata
     7321 tcctgttttt tctgactaac tggcaaattg tccggataag tgatttccgg tcgtgccgct
     7381 tcacgcagca ggactttccc tgccgcctgg tcaatctctt tcgccatctc ctggaaaatg
     7441 gcctgttgtg catcaggatt tttaaccttc ttaacaccat gcagacggcg agaaaaacgc
     7501 agtctgtcgc gcagcatcaa cgaatccagc cgctgctgca aagcagtaaa ggtcaatttt
     7561 tgttgttctg tcataacgtt agtgggcagt agtctgcctg attaattttc ttttaaatga
     7621 tagatataga gtaccacatc gcggctttct atgcgttgtt caataaattc gaacataggc
     7681 ttcgatttat tgcgctatct ctgtggcagg attgtgaata aagtgtcagc aagcaacggg
     7741 gcatcgccaa ttcaaacatc tataaggaaa caccatgagc aaggtattag ttcttaaatc
     7801 cagcatcctg gcagggtact ctcagtctaa tcagttgtcc gattattttg ttgaacaatg
     7861 gcgcgaaaag cactccgctg atgaaatcac cgttcgcgac ctggctgcaa atccgattcc
     7921 ggtactggat ggcgaactgg ttggcgctct gcgtccgagc gatgcgccgc tgactccgcg
     7981 tcagcaggaa gctctggcgc tttccgatga gctgattgcc gagctgaaag cccacgacgt
     8041 tatcgttatt gcggcaccga tgtataactt caacatctca actcagttga aaaactattt
     8101 tgacctggtt gcacgcgcag gcgttacttt ccgctatacc gagaacggtc cggaaggtct
     8161 ggtaacgggt aaaaaagcca tcgttattac cagccgcggt gggatccaca aagatggacc
     8221 aacggacctg gtgacgccgt atctgtccac gttcctcggc tttatcggca ttaccgatgt
     8281 gaaatttgtc ttcgccgaag ggatcgcata cggtccggaa atggcagcga aagcgcagtc
     8341 tgacgcgaaa gcggccatcg acagcattgt tgctgcataa ccggacacga tcttgatgtg
     8401 gactgtagtt cagtccacat ctcaatccac ttacctgttt tcccataatt tcagcatcgc
     8461 tttgtgatct tccttaagca caatatgcga acgtctggct cgaatataac taatcgcttc
     8521 atcaacggtt ttacagtgtc cgtaacataa caaccatgcc gccactacca gcgcactgcg
     8581 cgataatccc aacgcgcaat ggaccagaac gctgccttgc tcttcgcgta atgtttccag
     8641 catcgccacg gcctgtcgga gctccccctc ttccggaacc accagatcca gcatcggtac
     8701 acagaaatag agtcgatctt ttgtcgctcg tccccgaggg aattcaaagg tgacgtccag
     8761 aaccgcattc tgtgccggaa tatgtcgtgg aaacgcccct aaataaacac cagcagtaat
     8821 tttgctcacc ggctccaggc gacgacaaaa ccaacgcata gacagccaca tcccgatgcg
     8881 ccagggcaat gtcagccagt aaacggcggg cgggagtttc ccctgactat ctttccctgt
     8941 tgttatcgcg ccaagcccac cgtacccacg gccaataatg agtagcgata atactggcca
     9001 acataaccag actggccacc ataactgaat catcaccatt agctccatca acacaatgca
     9061 cgagcccgcg cctacgacat atggcagtgc tattttgatt cgacgttgat caggtttctg
     9121 ataattccaa cgacgatcga cgggcaccat ccagtcaatc aacataccta ccgccagccc
     9181 tgtgatgaca tcaataaaat gatgctgcca ggtcgtcaga gtagaaatag cgatgagtaa
     9241 aaaccatccg ccacagactt tacgccacct ctcagccaga tgctgacgaa agtgacgcca
     9301 aagtagccag cagagaataa tatgcagcga aggagactgg ttataaggca gatcaaacag
     9361 ttcaagttgc gaaaatggcc atcccgtcac cccactcact tcaggacgga taaaactaaa
     9421 cttcagcgga tagagcaaaa aaccgcagca ggccattacc gttgccagaa taagccggtg
     9481 gacaagtcgg cgctgttcga atgtcgtgct acaaacgaac agcgaaaatc catataaaag
     9541 atccagactc cagtaaggaa caatagtcca gggaagaaaa gggatcgccg tttcccaacc
     9601 gaatacctga ctggggatat catggctgtt aaggtcctga accgcggtga actgattaag
     9661 agatccatag gtgaagaaaa aaaacggggc cagcaacaat aaccagccag cgccttgtag
     9721 caatacgttc cgacgttcag ttatcacgac gcaccgccat cgaaaccgta aaaatacccc
     9781 actcatcaat ccgttgtgtg catttatcaa atccggcatc acgcacgagt gaatccatct
     9841 ccccttgcga acgcacgcgc attacccacg gtttaccatc tttatgactg gttaacaccc
     9901 cggcaatcat ctccagttga gggtgccacg gctgcccggt gtagatgaga atgccgaccg
     9961 gttcgatggc attggcaaga cctgcgagtg agttttttac ctgctcattt tcgggaaaaa
    10021 gctcatacag tccagagaca attgccagcg taggccgcgg agttaacgcg ctgagttcct
    10081 ccgggttaaa cgcatcgccc tgttcaaaac gcacccgccc agacattccc cgttgggcaa
    10141 tcatctcttg cccctgtgca acatttaact cgctgtaatc acgtaacaaa atatcgctta
    10201 cggcaggctc gtttgccagc gcatccagta catagcgccc atgccctgcg gcaatgtcaa
    10261 ccacgcggac ggctaaacct ttggcgtgga gatcggcaac ggcttgttta atcagtattt
    10321 gtaaatgggt tttgcgctgg cgaataccgc gccagccaac actgttcagg tagattttgt
    10381 cgattaagcg cccgaatgcg ttactacctt gcggttgatt ttgatagaca taatccagcg
    10441 aactgcctga atcaaagccg gtgctcattc cgagatgcag gcccgcagag tgcgtcccga
    10501 acaatttcat cgcctttcgc ataaagcgat acgctaaatc aaccggcgat aatagcacgg
    10561 gtccaccaga aagtagccgc cattgatccg ctgatggtcc tgtgcagtct tcatgctgat
    10621 aatcaaattt ttgcgatttg ttagcatata aacgactaat aaagctttgc attttttcaa
    10681 atgccagcgc cctgttctct tcacccaacg tgtcgtgata aaagcctggc agcagatgca
    10741 gctctttcag agggctacgt aatctctgat aaaaatcaat ctgcggttgg cgatgcacca
    10801 catagtcatc acctgatatc agaagttgcg tggggagcgt aatcgccgcc gcatctctaa
    10861 taatacgttc agacgtttta tagagatcga gcaagatatt gacggcaatc gcccgtgtga
    10921 tcagcggatc attattgaaa ctcgccaccc gttgccgatc gtgggtcaaa tagcgtcctt
    10981 tcacatagga attaataaaa aacagaccac gcagacgatg ccataacgcc agcgcaggac
    11041 gtgccagcgg cacatacaat ttaaccttaa aggccggaga agccagcacc agcccgcgaa
    11101 ttgcaggcgc ataatcatga acctatgtgg caaccagcac tgcgccgacg ctttgcgcga
    11161 tcacaaccac ctcttccagt ccaacctagc tgtcgctggc agcaaaacgg acaaattcat
    11221 cgacatcctg cactgaacgc gcaagagatg ggctataacc acgcggcccc gaagtttttc
    11281 catgcccccg agcatcccat gcataaaaag cagtatctgg catcgccagt tcatcaacga
    11341 tatgttgtag acgaccagaa tgttcatggc cacgatgaaa taagacaatg acctttttcg
    11401 cccct
//
LOCUS       DAZPEB010000134        11308 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZPEB010000134 DAZPEB010000000
VERSION     DAZPEB010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11308
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.135"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..283)
                     /locus_tag="ACHURJ_003005"
     CDS             complement(<1..283)
                     /locus_tag="ACHURJ_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR1281865.1"
                     /translation="MTESSDYESIQVFIGVDVGKDTHHAVAINRSGKRLFDKALPNDE
                     NKLRSLISDLKQHGQILLVVDQPATIGALPVAVARSEGVLVGYLPGLAMR"
     gene            500..1128
                     /locus_tag="ACHURJ_003006"
                     /pseudo
     CDS             500..1128
                     /locus_tag="ACHURJ_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006895405.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            1125..1739
                     /gene="prfH"
                     /locus_tag="ACHURJ_003007"
     CDS             1125..1739
                     /gene="prfH"
                     /locus_tag="ACHURJ_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFR1281866.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1796..3253)
                     /gene="pepD"
                     /locus_tag="ACHURJ_003008"
     CDS             complement(1796..3253)
                     /gene="pepD"
                     /locus_tag="ACHURJ_003008"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFR1281867.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3514..3972
                     /gene="gpt"
                     /locus_tag="ACHURJ_003009"
     CDS             3514..3972
                     /gene="gpt"
                     /locus_tag="ACHURJ_003009"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFR1281868.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            4064..5308
                     /gene="frsA"
                     /locus_tag="ACHURJ_003010"
     CDS             4064..5308
                     /gene="frsA"
                     /locus_tag="ACHURJ_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFR1281869.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIVMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            5366..5767
                     /gene="crl"
                     /locus_tag="ACHURJ_003011"
     CDS             5366..5767
                     /gene="crl"
                     /locus_tag="ACHURJ_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFR1281870.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5806..6861)
                     /gene="phoE"
                     /locus_tag="ACHURJ_003012"
     CDS             complement(5806..6861)
                     /gene="phoE"
                     /locus_tag="ACHURJ_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFR1281871.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGGEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAIGMTYQF"
     gene            7149..8252
                     /gene="proB"
                     /locus_tag="ACHURJ_003013"
     CDS             7149..8252
                     /gene="proB"
                     /locus_tag="ACHURJ_003013"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFR1281872.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            8264..9517
                     /gene="proA"
                     /locus_tag="ACHURJ_003014"
     CDS             8264..9517
                     /gene="proA"
                     /locus_tag="ACHURJ_003014"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFR1281873.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTCDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            9632..9707
                     /locus_tag="ACHURJ_003015"
     tRNA            9632..9707
                     /locus_tag="ACHURJ_003015"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9665..9667,aa:Thr,seq:cgt)
     gene            complement(9722..10840)
                     /locus_tag="ACHURJ_003016"
     CDS             complement(9722..10840)
                     /locus_tag="ACHURJ_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1281874.1"
                     /translation="MPNGKRFKQSLGTKDKRQATELHDKLKAEAWRVSKLGEIPDITF
                     EEACVRWLEEKAHKKSLDDDKSRIGFWLQHFAGMQLRDITESKIYSAMQKMTNRRHEE
                     NWKLRAEACRKKGKPVPEYTPKPASVATKATHLSFIKALLRAAEREWKMLDKAPIIKV
                     PQPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSNIINLEWQQIDMQRRV
                     AWINPEESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKESCTKPDGTKAPTVRKM
                     RYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVLQEMGGWESIEMVRRY
                     AHLAPNHLTEHARQIDSILNPSVPNLSQSRNKERTNDV"
     gene            complement(10762..11196)
                     /locus_tag="ACHURJ_003017"
     CDS             complement(10762..11196)
                     /locus_tag="ACHURJ_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1281875.1"
                     /translation="MGYDSRLDRLATTSWHSFFNNITARGEIMEPYSLTLDEACHFLK
                     ISRPTAINWIRTGRLQATRKDPTKNKSPYLTTRQACIAALQSPLHTVQVSAGDGITEE
                     RKCHSSAEVKYGTPVSHCRTVKDLNSLLEQRTKGRRQNSMTS"
     gene            complement(11112..>11308)
                     /locus_tag="ACHURJ_003018"
     CDS             complement(11112..>11308)
                     /locus_tag="ACHURJ_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1281876.1"
                     /translation="LEVEPDERESYELFMEKHFGGLVDSRRAKNGDNEYMAWDMTLGW
                     IVWQQRAGIHFSTISQQEVK"
BASE COUNT         2690 a         2793 c         2927 g         2898 t
ORIGIN      
        1 ggcgcatggc cagtccaggg aggtatccga caaggactcc ttctgagcgg gcaacggcga
       61 caggtaacgc accgatggta gctggctgat caacaaccag cagtatctga ccatgttgtt
      121 tcaggtcaga tattagcgac ctgagtttgt tttcgtcgtt gggtaatgct ttatcgaaca
      181 ggcgtttacc tgaacgatta atggcaacag cgtgatgcgt atctttaccg acatcaacgc
      241 cgataaagac ctggatggat tcgtaatcgc tggattcggt cattctgtct cccttgtata
      301 tgggttaacc agataaccac ggggagcagg taccggcatc cacgttacag acggtcccgg
      361 caaaagtgcc tgacctgacc tctattagcg gttaccagcg ccccaccaga cccggtgaca
      421 tcaccccccg gatcatggac gactgggggc agtaatcatg ccgggtctgg ctggctaaca
      481 ccccattata aggggtacga ataaagtaac ggggcagtct attttacagc ggcatattgc
      541 ctcgttctcg catcatggtt tgcctcaagg cagtgacgac gcgctacggt atattgcgga
      601 acatgacgat gcgctggcgt ttgcgtgtat taatcgccag atgatcgctt tgcgcataat
      661 gcaacaagtt aaggccacgg gaagtccggt tctggatgtg gcgcataact ttgttagcgc
      721 gtgtcaaatc ggcgtcaaca gggctggttg catcgtaaag gtgccacgcc ggatgacaac
      781 ggtctggtga ttattcccgg ttcacgtggt gattactcct ggattgtaca gcctgttgcg
      841 aacgaaaaaa cgctccactc gctggcgcat ggggcagggc gtaaatgggg gcgcaccgag
      901 tgtaaatggc gtctggcagc gaaatacacg gtgacgcaac tctcgcggac tgaacttggc
      961 agtcggataa tttgtcgcga taaacaactc atctttgaag aagcgccaca agcctataaa
     1021 tcggctgaaa gcgtggtgca atgtctggtg caggccggtt taattattcc tgtcgcgcgg
     1081 ctgcgtccgg tgctaacgct caaaaacagt ggagggaaaa aaggatgatc ttgctacaac
     1141 tctcctcagc gcaggggccg gaagaatgtt gtctcgcagt gaaaaaggcg ctggacaggc
     1201 taattaaaga agctgcccga caagacgtcg cggtaacggt gttggaaaca gaaacgggcc
     1261 gctactctga cacgctgcgt tcagctctgg tttctctgga tggcgataac gcatgggcat
     1321 taagcgaaag ctggtgtggc actattcagt ggatttgtcc gagtccgtat cggcctcatc
     1381 atgggcgcaa aaactggttt ctgggcattg ggcgttttac cgctgatgag caggagcaat
     1441 ccgatgcgat ccggtatgag acgctgcgtt cgtcggggcc gggcggtcaa catgtcaata
     1501 aaaccgactc ggcggtacgc gccacgcatt tggcatccgg tattagcgtg aaggttcagt
     1561 cagagcgtag tcagcatgct aacaagcggc tggcacgatt gctgattgcc tggaagctgg
     1621 agcagcagca acaggaaaat agcgcggtgc tgaaatcgca gcggcgaatg ttccatcacc
     1681 agattgaacg tggcaacccg agacggacat ttacagggat ggcttttatc gaaggataat
     1741 gaaggatgaa actgccggaa ggcgattaaa cgccatccgg cagcaaatca aataattact
     1801 tcgccggaat ttctttcagc agttcagtca gcagtgtcca gtaatgacct acgctttcga
     1861 tgtgaacttg ctcatccgga gagtgtggac cggtgatagt tggcccgata gaaaccatgt
     1921 ccatttccgg atacggtttt ttgaacagac cacattccag gcccgcgtgg ataatctgga
     1981 tgttcggcgt cttgttgaac aggcgctgat aggtttcacg taccagatgc atcaccggag
     2041 aattagcgtc cggctgccag ccaggatatg cgcctttcgc ttcggttttc gcgccagcca
     2101 gtttacccag cgaatccagc atgctcacca cgtagtcttt accgctgtcg atcagtgaac
     2161 ggatcaggca gtgaatttct acgttattgt cagtcatggt caccacaccg acgttcaggg
     2221 aggtttcaac cacacctttg gctacatcgg agttgcggat cacaccgttc ggggtggcgt
     2281 tcagcagacg aataaaggta tcgcgagatt tcgcaatcag ggcagcttta tcgttcgcta
     2341 cagagtccag caacaaggcc agatttttct ctttctctgc cagctcgttt ttcaggatct
     2401 cctgataggt attcaccaga gatttcagtg cgtcgacttt atcagctgcg acagcaatgg
     2461 tcgcaaaggc ttcacgcggg atggcgttac gcagtgtgcc gccgttgaaa tcgataaggc
     2521 gcaggtccag ttcttctgca tgacccgcca ggaagcgcac cagcagtttg ttggcattac
     2581 ccaggccaac gtggatttca ccgccggagt gaccgccttt cagacctttt aaggttaact
     2641 tgaaggtttc aaaaccagct ggaaccgctt cacgatctaa atgcaggttg gaggtgaagt
     2701 cgataccccc cgcacaaccc atgtagattt caccttcttc ttcggagtcg gtgttaatca
     2761 gaatatcagc ctgcaaccag ttgctctgta agccaaaagc accgtccata ccggcttctt
     2821 cggtcatggt cagcagcact tccagcggac cgtgaaccac gttttcgtca gccagaaccg
     2881 ccagtgcaga ggccatacca atgccgttat ccgcacccag cgtggtgccg cgcgctttaa
     2941 cccattcgcc atcaatataa ggctggatag gatctttcgt gaagtcatgc acggtgtcgt
     3001 tatttttctg cggcaccata tcgaggtggg cctgtaagac gaccggttta cgattttcca
     3061 tacctgcggt agcaggttta cgaatcagga tattacctac ctgatcgcgt tcgacatgga
     3121 aacctttctc ttttgcccaa ccaacaatgt attcagcgag ttgctcttca tgataggacg
     3181 ggtgcggaat agaacagatt ttggcaaaaa tatcccacag cggctgtgga gataattgag
     3241 acagttcaga cacgttaagt ctccttgtcg atcacccgca aaacagtatt gcaggtcaca
     3301 gggttagcag aaaatgttgt caacacaaga caggcttgcg agatatgttt gagaatacca
     3361 ctttatcccg cgtcagggag aggcagtgcg taaaaagacg cggactcatg tgaaatactg
     3421 gtttttagtg cgccagatct ctataatctc gcgcaaccta ttttcccctc gaacactttt
     3481 taagccgtag ataaacaggc tgggacactt cacatgagcg aaaaatacat cgtcacctgg
     3541 gacatgttgc agatccatgc acgtaaactc gcaagccgac tgatgccttc tgaacaatgg
     3601 aaaggcatta ttgccgtaag ccgtggcggt ctggtaccgg gtgcgttact ggcgcgtgaa
     3661 ctgggtattc gtcatgtcga taccgtttgt atttccagct acgatcacga caaccagcga
     3721 gagcttaaag tgctgaaacg cgcagaaggc gatggcgaag gcttcatagt tattgatgac
     3781 ctggtggata ccggtggtac tgctgttgcg attcgtgaaa tgtatccaaa agcgcacttt
     3841 gtcaccatct tcgcaaaacc ggctggtcgt ccgctggttg atgactatgt tgttgatatt
     3901 ccgcaagata cctggatcga acagccgtgg gatatgggcg tcgtattcgt cccgccaatc
     3961 tccggtcgct aatcttttca acgcctggca ctgccgggcg ttgttctttt taacttcagg
     4021 caggttacaa tagtttccag taagtattct ggaggctgca tccatgacac aggcaaacct
     4081 gagcgaaacc ctgttcaaac cccgctttaa acatcctgaa acctcgacgc tagtccgccg
     4141 ctttaatcac ggcgcacaac cgcctgtgca gtcggctctt gatggtaaaa ccatccctca
     4201 ctggtatcgc atgattaacc gtctgatgtg gatctggcgc ggtattgacc cacgtgaaat
     4261 cctcgacgtc caggcacgta ttgtgatgag cgatgctgaa cgtaccgacg atgatttata
     4321 cgatactgtg attggctacc gtggtggcaa ctggatttat gagtgggcca cccaggcgat
     4381 ggtgtggcaa caaaaagcct gtgcggaaga agatccgcaa ctcagtggtc gacactggct
     4441 gcatgcggct acgttgtaca acattgccgc ctatcctcat ctgaaaggag atgacctggc
     4501 cgagcaagcg caggctttat caaaccgcgc ctatgaagag gccgcccagc gtctgccggg
     4561 cacgatgcgg cagatggagt ttaccgtacc cggcggtgcg cccatcaccg gctttttgca
     4621 tatgccgaaa ggcgatggcc cgttcccgac agtattaatg tgtggtggtc tggatgcgat
     4681 gcagacggac tattacagcc tgtatgaacg ttattttgcg ccgcgcggca ttgtgatgct
     4741 gactattgat atgccgtcgg tgggcttttc ttcaaaatgg aagctcaccc aggactccag
     4801 cctgttgcat cagcacgtct taaaggcgct gcctaacgta ccgtgggtgg atcacactcg
     4861 cgtcgcggcc tttggtttcc gtttcggcgc taacgttgcc gtgcgtctgg catatcttga
     4921 atcgccgcgt ctgaaagcgg ttgcctgtct tggtccggta gttcataccc tgttgagtga
     4981 ttttaagtgc cagcaacagg tgccggaaat gtatcttgac gttctggcga gtcgtttggg
     5041 gatgcatgat gcttccgacg aagcgttacg cgtggagctg aatcgctatt cattaaaagt
     5101 gcaaggattg ctgggacgtc gctgcccaac gccaatgtta tcaggctact ggaagaacga
     5161 tccgttcagc ccggaagagg actcacgctt aatcacctca tcatctgctg acggtaaatt
     5221 attagagatc ccatttaacc cggtgtatcg gaattttgac aaaggtcttc aggaaatcac
     5281 cggctggatc gaaaaacgct tgtgttaaaa atttgctaaa ttttgccaat ttggtaaaac
     5341 agttgcatca caacaggaga tagcaatgac gttaccgagt ggacacccga agagcagatt
     5401 gatcaaaaaa tttaccgcac taggcccgta tattcgtgaa ggtaagtgcg aagataatcg
     5461 attctttttc gattgtctgg ctgtatgcgt caacgtgaaa ccggcaccgg aagtgcgtga
     5521 attctggggc tggtggatgg agctggaagc gcaggaatcc cgttttacct acagctacca
     5581 gtttggtctg ttcgataaag caggcgactg gaagagtgtt ccggtaaaag atactgaagt
     5641 ggttgaacga ctggagcaca ccctgcgtga gtttcacgag aagctgcgtg aactgctgac
     5701 gacgctgaat ctgaagctgg aaccggcgga tgattttcgt gacgagccgg tgaagttaac
     5761 ggcgtgagtg aaatgtgccg gatgcatcac atccggcaat atctattaaa actgatacgt
     5821 catcccaatc gcgacaatat catcattatt aatattcaat ttgttatcgc tatccagttg
     5881 gttgatttta taatcaacaa atgctgacat atttttgttg aaataatacg tagcaccgac
     5941 gtcgatataa ttaaccagat cttcaccacc gatgccctca atatctttcc ctttcgataa
     6001 gacataaccc agcgatggac gcagaccaaa gtcaaactgg tattgagcga ccgcttcaaa
     6061 gttctgtgtc ttattggcaa agccgccagt tattggcgtc attttgcgag tttcagaata
     6121 gaaagttgcc agataaatat tattggcatc gtatttcaga cctgttgccc atgcttctgc
     6181 acgcttgcct gtgccacggc tttgcaggtt ctgctcgttg gtgcgatctg agttggtata
     6241 ggccccacta atggcgaaat cgctgccgcc aaagtcatat gtcaatgacg tgccgaagcc
     6301 atcgccgttt tgctttttaa cgtcgcggtt ttcgtttttc ccttgatatt gcagggttaa
     6361 gttcaggcca tcgataacgc cgaagaagtc ggtgttccga tacgtcgcca gaccgctggc
     6421 gcgtttggtc ataaagttgt cggtctgcgc cgaggagtcg ccaccaaatt ccggaaacat
     6481 atcggtccag gcttccacgt catacaacgc gcccaggtta cgaccataat cgaaagaacc
     6541 caaatcttta tatttcaacc cggcaaaagc gagacgcgtt ttttgctgtg cagtatcact
     6601 ctctgcttta ttaccggcaa actctgcttc ccaacgacca taaccagtca gttgatcgtt
     6661 aatttgtgtt tcgcctttaa aaccaaaacg gatataactc tggtcgccat ctttactgtc
     6721 gttatcactc atataatgca tggctttaac tttgccatag acatccagtt tattaccgtc
     6781 tttgttatat atttctgcgg cctgtacgga tgcagatgcc acaatgccca tcaccactaa
     6841 tgccagagtg ctctttttca ttttcattcc tgattttaat taacgcgcga atattcagcg
     6901 ggagagcccc gttgaaaaca ggaaagtttt taacctgaga ttgttaaaga tatattacag
     6961 attaataata ttcttaaaat gtggtaattt attaaatctg taataaaagc gtaaacaact
     7021 gccgctaggc ttgctgatcc cgcgcaacaa aacgccatgc tttgctcgca gatggttggc
     7081 aaccgacgac agtcctgcta aaacgttcgt ttgatatcat ttttcctaaa attgaatggc
     7141 agagaatcat gagtgacagc cagacgctgg tggtaaaact cggcaccagt gtgctaacag
     7201 gcggatcgcg ccgtctgaac cgtgcccata tcgttgaact tgttcgccag tgcgcgcagt
     7261 tacatgccgc cgggcatcgg attgttattg tgacgtcggg cgcgatcgcc gccggacgtg
     7321 agcacttggg ttacccggaa ctgccagcga ctatcgcctc gaaacaactg ctggcagcgg
     7381 tagggcagag tcgactgatt caactgtggg aacagctgtt ttcgatttat ggcattcacg
     7441 tcgggcaaat gctgctgacc cgtgctgata tggaagaccg tgaacgcttc ctgaacgccc
     7501 gcgacaccct gcgtgcgttg ctcgataaca atatcgttcc ggtaatcaat gagaacgatg
     7561 ctgtcgctac ggcagagatt aaagtcggcg ataacgataa cctttctgcg ctggcggcga
     7621 ttctggcggg tgccgataaa ctgttgctgc tgaccgatca aaaaggtttg tataccgctg
     7681 acccgcgcag caatccgcag gcagaactga ttaaagatgt ttacggcatt gatgacgcac
     7741 tgcgcgcgat tgccggtgac agcgtttcag gcctcggaac tggcggcatg agtaccaaat
     7801 tgcaggccgc tgacgtggct tgccgtgcgg gtatcgacac cattattgcc gcgggcagca
     7861 agccgggcgt tattggtgat gtgatggaag gcatttccgt cggtacgctg ttccatgccc
     7921 aggcgactcc gcttgaaaac cgtaaacgct ggattttcgg tgcgccgccg gcgggtgaaa
     7981 tcacggtaga tgaaggggca actgccgcca ttctggaacg cggcagctcc ctgttgccga
     8041 aaggcattaa aagcgtgact ggcaatttct cgcgtggtga agtcatccgc atttgcaacc
     8101 tcgaaggtcg cgatatcgcc cacggtgtca gtcgttacaa cagcgatgca ttacgccgta
     8161 ttgccggaca ccactcgcaa gaaattgatg caatactggg atatgaatac ggcccggttg
     8221 ccgttcaccg tgatgacatg attacccgtt aaggagctgg ccgatgctgg aacaaatggg
     8281 cattgccgcg aagcaagcct cgtataaatt agcgcaactc tccagccgcg aaaaaaatcg
     8341 cgtgctggaa aaaatcgccg atgaactgga agcacaaagc gaaatcattc tcaacgctaa
     8401 cgcccaggat gttgctgacg cgcgagccaa tggccttagc gaagcgatgc ttgaccgtct
     8461 ggcactgacg cccgcacggc tgaaaggcat tgccgacgat gtacgtcagg tgtgcaacct
     8521 cgccgatccg gtggggcagg taatcgatgg cggcgtactg gacagcggcc tgcgtcttga
     8581 gcgtcgtcgc gtaccgctgg gagttattgg cgtgatttat gaagcgcgcc cgaacgtgac
     8641 ggttgatgtc gcttcgctgt gcctgaaaac cggtaatgcg gtgatcctgc gcggtggcaa
     8701 agaaacgtgt cgcactaacg ctgcaacggt ggcggtgatt caggacgccc tgaaatcctg
     8761 cggcttaccg gcgggtgccg tgcaggcgat tgataatcct gaccgtgcgc tggtcagtga
     8821 aatgctgcgt atggataaat acatcgacat gctgatcccg cgtggtggcg ctggtttgca
     8881 taaactgtgc cgtgaacagt cgacaatccc ggtgatcaca ggtggtatag gcgtatgcca
     8941 tatttacgtt gatgaaagtg tagagatcgc tgaagcatta aaagtgatcg tcaacgcgaa
     9001 aactcagcgt ccgagcacat gtaatacggt tgaaacgttg ctggtgaata aaaacatcgc
     9061 cgatagcttc ctgcccgcat taagcaaaca aatggcggaa agcggcgtga cattacacgc
     9121 agatgcagct gcactggcgc agttgcaggc aggccctgcg aaggtggttg ctgttaaagc
     9181 cgaagagtat gacgatgagt ttctgtcatt agatttgaac gtcaaaatcg tcagcgatct
     9241 tgacgatgcc atcgcccata ttcgtgaaca cggcacacaa cactccgatg cgatcctgac
     9301 ctgcgatatg cgcaacgccc agcgttttgt taacgaagtg gattcgtccg ctgtttacgt
     9361 taacgcctct acgcgtttta ccgacggcgg ccagtttggt ctgggtgcgg aagtggcggt
     9421 aagcacacaa aaactccacg cgcgtggccc aatggggctg gaagcactga ccacttacaa
     9481 gtggatcggc attggtgatt acaccattcg tgcgtaaata aaaccgggtg atgcaaaagt
     9541 agccatttga ttcacaaggc cattgacgca tcgcccggtt agttttaacc ttgtccaccg
     9601 tgattcacgt tcgtgaacat gtcctttcag ggccgatata gctcagttgg tagagcagcg
     9661 cattcgtaat gcgaaggtcg taggttcgac tcctattatc ggcaccattt aaatcaataa
     9721 gttacacatc attagtacgt tccttatttc ttgactggga caaatttggg accgatgggt
     9781 tcagaatcga gtctatttgc cgtgcgtgtt cggtaaggtg attaggtgcg aggtgagcat
     9841 atcgacgaac catttcgata gactcccagc ctcccatttc ctgtaacact gataacggga
     9901 ctccggcttg aaccagccaa cttgcccagg tgtgtctcaa gtcgtgaaat ctgaaatcat
     9961 caatacctgc tcgtctcagc gccgctttcc aggctgtgtt tgcgtcatac cgcatcttcc
    10021 tgactgttgg cgctttcgtt ccgtctggtt tggtacagct ttccttatac acaaataccc
    10081 aacggtgatg attcccgatt tgttttttca atacgcgaca tgcagtatca ttcagcgcaa
    10141 cgccaattgc gcggtttgat ttactctctt ccgggtttat ccatgccacc cggcgctgca
    10201 tgtctatttg ttgccattca aggttgatga tgttcgagcg tcttaagcct gttgcaagtg
    10261 caaattcaac aacagacttt aatggctccg gacattcatc aatcagcctt tgtgcttcat
    10321 gaggctccag ccagcggatc cgtttattct ttggttgagg cactttaata attggtgcct
    10381 tatccagcat tttccattca cgctctgcgg ctcttagcag ggcctttata aatgaaagat
    10441 gcgtagcctt cgttgcaacg gacgctggtt ttggcgtgta ttctggaaca ggtttccctt
    10501 tttttctgca tgcttctgcc ctgagtttcc agttttcctc atgacgccgg ttcgtcattt
    10561 tctgcattgc tgaataaatt tttgattcag taatgtctct tagttgcatt cctgcgaaat
    10621 gttgaagcca gaatccgatc cggcttttgt catcgtccag tgatttttta tgtgctttct
    10681 cttcaagcca cctgacacac gcttcctcga acgttatatc aggaatttca ccaagtttgc
    10741 tgacccgcca tgcttcagcc tttagcttgt catggagttc tgtcgcctgc cttttgtcct
    10801 ttgttccaag agactgttta aatcttttac cgttcggcaa tgtgaaactg gcgtaccata
    10861 tttcacctct gcggaagagt gacattttct ttcctctgtt atgccatcac ccgcgctcac
    10921 ctggacagta tgcagcggag actgaagcgc cgcaatgcag gcttgtcgtg ttgtgaggta
    10981 aggagattta ttcttagtgg gatctttgcg tgttgcctgc agacgccctg tgcgtatcca
    11041 gttaatggca gtcggtctgg atatcttgag aaaatgacag gcctcatcta gcgtgaggct
    11101 gtatggttcc attatttcac ctcttgctgt gatattgttg aaaaatgaat gccagctcgt
    11161 tgttgccaga cgatccaacc gagagtcata tcccatgcca tgtattcgtt atcgccgttt
    11221 tttgctctcc gactatctac taagccaccg aaatgttttt ccataaataa ttcataggat
    11281 tcgcgttcat ctggctctac ttccagag
//
LOCUS       DAZPEB010000135        10933 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZPEB010000135 DAZPEB010000000
VERSION     DAZPEB010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10933
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.137"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            223..531
                     /locus_tag="ACHURJ_003019"
     CDS             223..531
                     /locus_tag="ACHURJ_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001776105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1281877.1"
                     /translation="MSMQTVRRKALRQNPYSTLGKPPLRSSRDRITRAINGTWSLREL
                     PDNVRELLIERAALRAEITTLTVKAQGLKKSDVSVLIADRLRRIDAITSLLENRKCNA
                     "
     gene            524..739
                     /locus_tag="ACHURJ_003020"
     CDS             524..739
                     /locus_tag="ACHURJ_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1281878.1"
                     /translation="MPDITPAPASLLEAVFTPMTEYTHNVYSVKGDRLAMTLTKIGVA
                     QANSQGDIQLFINPDCATGKVLLYRKD"
     gene            742..1404
                     /locus_tag="ACHURJ_003021"
     CDS             742..1404
                     /locus_tag="ACHURJ_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFR1281879.1"
                     /translation="MLTTAALFQLAMQCAPAVHPDTIHDITRTESGLNPYAIAEIVPV
                     KGGRSRVISHLPSSKDEALKIVEAIKQKKHRYSVGLMQITSTNFPQFGVSAESMFNPC
                     DNMTVAAKIITDCYQRGGTLQRALSCYYSGNFETGQRPESAFGNTSYVQRIGYVVPST
                     RAEQQTASPSSGEAPAVPSDNTVYPDSVIRGVIPAPDTTPTSIAAYPPHVVRGGLAVS
                     SD"
     gene            1415..1714
                     /locus_tag="ACHURJ_003022"
     CDS             1415..1714
                     /locus_tag="ACHURJ_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HFR1281880.1"
                     /translation="MTLRTIKTAATRLIAAFSALSAVPAMAASADQAFQKGSDAINWV
                     FLGLTGMSVVTISCAVAFIAYKMLVDGKAWHDLQRVFWAGVLLAGASGFGAYFAA"
     gene            1724..4474
                     /locus_tag="ACHURJ_003023"
     CDS             1724..4474
                     /locus_tag="ACHURJ_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="HFR1281881.1"
                     /translation="MTILNKALTRPAALWGVPLVPLLFTLCILTLAAVWSRFYWLLLL
                     LIPAFIELRRLAKEDIHFFDLKLLAFKTRGNRAATRHFGTKALVANQYDAIDVTEFIK
                     HMRLNESIPLSRYLPWSSHLHEQVVKNKKGDFVSTWEIGGIIFECEDEHYLATLDMQL
                     NNVIRSFEGLPVTFYTHLVSDEFTDTLECESGIPFSDEIARLFYASREGKPFYQRRLF
                     FTVCFNPHTGIERATMKQKSLGERKTLLDNALQVMQEHRQAIGTALSRYAARPLGTYE
                     ENGHVFSSQLAFYNFLITGRWQKVRVTRTPFYDVLGGTDIFPSTDIGQVSHTDGQSYF
                     RGLEIKDYSTETITGLYDSLLYARCRYVFTQSYTCMGKDEARGCIRTIEKRLKSAEDD
                     AISQQEDLKVARDMLQSGIISFGKYHQTLIVSAPTSEQVIRDTNTLADTLKDLGFIMT
                     LSRESLSAAYLAQLPGVYHLRPRLVPVSSLNYAEMVSFHNFHPHKRDGNAWGEAIAVV
                     PTPSGGCQYVNLHNSQADVDEFSEKPPGNTMLLGSTGTGKSTLLMMFQHLMQKYRRPE
                     SFAPGATKKRFTSVVFDKDRSAEMSIRLQGGSYFRFRSGEKTGLNPFALAPTKRNRNF
                     LKKLMRMMCTRDGKPLDPRDEERLSEAVDTIMLDYPPELRRNGITRMLEVRHEAPTKD
                     ARMNGLRIRLQQWAQGGEFGWVFDNDVDTFDIDNIDNFGIDGTEFLNDKDVRSPLTFY
                     LLYRVTSLLDGRRLVIFMDEFWKWLQDPEFSKFALDMLKVIRKLNGIFFPATQSPEEM
                     CKHEISAAIIEQCSTRIFMANQQAKEYDYVDMLRVPKAVYDIVTTLDPYARQMVIMKS
                     PLRRGDVRPYIAQITLDLSGLGRYTKLLSASADNLKIFDEIWQEGMKPEDWKDEMLRR
                     AV"
     gene            4493..5209
                     /locus_tag="ACHURJ_003024"
     CDS             4493..5209
                     /locus_tag="ACHURJ_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HFR1281882.1"
                     /translation="MKLKTTLTALTVALCLFHVPAQAGIPVFDEVANASNIQQWAEKL
                     QQWQQTVQHYQSQMNAYKQQLATATGIRDVQAFLNQAKNLTADIKNLKNRGISLNDLL
                     TGSGGSYAGELNRLYDKYKLFDNCPENASEAYRDSCKQMLLNQAVSVEDTSDIQEKIS
                     DTLSDISNLASRIENAKDSKESQDLANVVSARSIQLNALTTQWEMSVKQSEMRNQLLA
                     EQRRKAQAEQMRTAPIADLN"
     gene            5221..5505
                     /locus_tag="ACHURJ_003025"
     CDS             5221..5505
                     /locus_tag="ACHURJ_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001776107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="HFR1281883.1"
                     /translation="MKLKTLTFIVLASVAALTGCEDKTSVDWYMAHHDDMLAKYRECI
                     FNHNFVPTDCQNARSALHREKAKPDVAEGYKQILKDLDKARVQEPIADLN"
     gene            5523..6530
                     /locus_tag="ACHURJ_003026"
     CDS             5523..6530
                     /locus_tag="ACHURJ_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030255 - protein secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HFR1281884.1"
                     /translation="MSGIFVGLNSHIMEGFDSVLTSVAADTGGWMAHLFSIAVLSLYI
                     TWRAYQTMAGKLVRPFEEIVWDVSRMLIIMIFVTNAGGYLDLVSDAITGLRDGFSGDA
                     SIWARLDTVWEKAQALGEKLYSYDDSTYVALKGTLAQFLVWAGVMLLVGITAIINLIA
                     ELVMLLMLATAPIFIYCLMWSWFRDMFSNWLRIILGCILTSLFSGIALTVVMDYLDYI
                     LAGATKASDANYLTLAVQVFLGTAGAAAVMVLTYKLAEALAGASAQGAAQALAASGVG
                     SARQSASSAAGAVGDKASQNLNSTGSATASSASTASRATGDARSRSRAAVQRMQERYQ
                     R"
     gene            6607..6747
                     /locus_tag="ACHURJ_003027"
     CDS             6607..6747
                     /locus_tag="ACHURJ_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1281885.1"
                     /translation="MKYGITLLMLCLLAGCAQRNTQLPPVSGNPEPVNSPAIIQELTP
                     HV"
     gene            6740..7426
                     /locus_tag="ACHURJ_003028"
     CDS             6740..7426
                     /locus_tag="ACHURJ_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virB8 family protein"
                     /protein_id="HFR1281886.1"
                     /translation="MSELTAKALSDSRSFENAILERDMRMKKAGWIVAALAGLLLVFA
                     LAALILLLPLKTTTVELYTLDRQTGRIERVTTVGEDKLDTSEVLNLSQTASYVKRREG
                     YNYFALQQDYRETQMFNSDEVNKEYLDWFNSPDAPDAVFQQAAYVVTVEVLSNVPSEG
                     TGSSMVEMLRIKRTIRKVKDNTETYDYWTVRMTYRYFPQKKMTASEREVNPFGFIVTS
                     YQRFKEKSDE"
     gene            7419..8312
                     /gene="virB9"
                     /locus_tag="ACHURJ_003029"
     CDS             7419..8312
                     /gene="virB9"
                     /locus_tag="ACHURJ_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001045307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein VirB9"
                     /protein_id="HFR1281887.1"
                     /translation="MNKVLMCALLLTAASAWGAATPRGSAYDSRMQNIPYNSQNTTVI
                     NTRAGYITTLVFDADEEVITATAGFPQGWTITPDRNRVGISPAPVKQPVTDADGKTVQ
                     KIFTPTQQDWQTNLFVTTTKRDYSLMLNVIDNDREAGNLAFVVRFSYPDDTRRQNDAA
                     RLARQQELQAAQEKERIQFALKKSTAPRNWNYTRRVAAGSESITPDFAYDDGRFTYLG
                     FSPQKLIPSPTVIVGGSEQVITPTFSTEGNYRVMVLRSLSPRFVLRYGSQVVGIENGS
                     YGKVTVANGSTVSPDVKLESK"
     gene            8309..9505
                     /gene="virB10"
                     /locus_tag="ACHURJ_003030"
     CDS             8309..9505
                     /gene="virB10"
                     /locus_tag="ACHURJ_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000980841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB10/TraB/TrbI family type IV secretion system
                     protein"
                     /protein_id="HFR1281888.1"
                     /translation="MMNEQEDIKTAAELEAEARERARAEIDGDKPAAGAVPPGQPQVV
                     QHAKRRSRRSLVLAAISLVLLVIIATGGDYLMAKLKGDGSGKSASHEPESHATSPARE
                     RNNLGRDLNPLGQLADTTGQEKTTPTDTAAPPQASPPPLTFDKVSALADSAPTTASSA
                     PKSTSSSNVSPAGSASAPGSATPAGQSQDNGLARITGVKRLGLDPDLYIPADRHIPCA
                     MMQRFVSDVGGRFNCLISEDIYSASNAVRLIPAGTEARGLYRTGTLKNGQGRLFLAIT
                     ELRTPEPGRLVIPMVDSQAVGALGENGVAGWIDNHWLERIGNTLLLGTVQDFAAAASG
                     SSPGKDRNTDYTENTRAATAEMAKTLLENSINIPPTMYLNQGDVIGLVTGADIDFSDV
                     YRLRMR"
     gene            9509..10558
                     /gene="virB11"
                     /locus_tag="ACHURJ_003031"
     CDS             9509..10558
                     /gene="virB11"
                     /locus_tag="ACHURJ_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043684 - type IV secretion system
                     complex [Evidence IEA];
                     GO_process: GO:0044097 - secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="HFR1281889.1"
                     /translation="MDDKLTDTPSEGNVALSHLRDLLFGDYLSREGLTEIAINRPGEL
                     HTKINGAWEMHASPVTLRQCSSFARALAVWNNDTIDETSPILSATLPTGERCQIVVPP
                     ACERNTISITLRKPDYFQRTHQSYIDAGFYDRVQGTEKVESKDGELSRIYLSGNIPLF
                     MEKCVEYGKTLFVVGETGSGKTTYMKMLLHYIPETLRLITIEDNPELKFYSQKNYVHL
                     FYPADAGEGAIVTSDRLVRAIYRMNPDRPLLAEVRGREAWDALKLIESGHEGFMSSLH
                     AGSPRECISGLIDRCYEHPACRGMPVDVLLRKVLHCVDVIVSVDVHGNVRRMSDVYFK
                     PLHMQAMKESFNDAA"
     gene            10545..>10933
                     /locus_tag="ACHURJ_003032"
     CDS             10545..>10933
                     /locus_tag="ACHURJ_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cag pathogenicity island Cag12 family protein"
                     /protein_id="HFR1281890.1"
                     /translation="MMLPKLTGLTAIVVLLAGCSSPPEPAAVDWSQPAQPVTTTRPVW
                     EPNRLIVASPVTEGHWSQVLTRFEPGGIYPAAQWYAVAHASRAVVNAPDGQRFFAAKT
                     WLREGGYKGVVEFRPRSGCLTCDTSEIV"
BASE COUNT         2418 a         3298 c         3092 g         2125 t
ORIGIN      
        1 tattgtatta atcaaatcat tagctcttta ataacctagc agcggcgttc cccggtcagg
       61 ataaggaggt gttttctgtg actgcgtaaa gagatgacgt gccgcgcctg taatgggtaa
      121 cactctcact cctctgtatc gttaagcctc ccgtttaatc cttccctgtc tcccactgtg
      181 caccgcacgc gggtggctta ctcataccca ggagaaacag ccatgagcat gcaaaccgtc
      241 cgccgtaagg cgctgcgtca gaatccttat tcaactctcg gcaagccgcc gctacgctcc
      301 tcccgtgacc gtatcacgcg tgccattaat ggcacctggt cgctcaggga gctgccggat
      361 aacgtccgag aattgctaat tgaacgggca gccctgcggg cagaaatcac taccctgact
      421 gtgaaagcgc agggactcaa aaaatccgat gtatccgtac ttattgccga caggctgcga
      481 cgtatcgatg caatcacctc actgctggaa aataggaaat gtaatgcctg acatcacgcc
      541 tgctccggcc tcactgcttg aagccgtatt cacccccatg accgagtaca cgcacaacgt
      601 gtattcggtg aagggtgatc ggctggcaat gacgcttaca aaaatcggtg ttgcccaagc
      661 taacagccag ggcgacattc agttatttat caacccggac tgtgccaccg gtaaggtact
      721 gctctacaga aaggactgac tatgttaacc actgccgcgt tgtttcagct cgcaatgcag
      781 tgtgctcccg ccgttcaccc cgataccatc cacgacatca cccgcaccga atccggctta
      841 aatccatatg ccatcgctga gattgtcccg gtaaagggcg gcaggagccg cgtgatttct
      901 catctgcctt cgtcgaaaga cgaggcgcta aaaattgtgg aggccataaa gcagaaaaag
      961 caccgttatt cagtagggct gatgcagata accagcacca acttcccgca gttcggcgta
     1021 agcgccgaat ccatgtttaa cccctgtgac aacatgactg ttgccgcgaa aatcatcacc
     1081 gactgctatc aacgaggagg aacattacag cgcgccctga gctgctatta ctcaggcaac
     1141 tttgaaaccg gccagcgccc ggaaagcgcg ttcggcaaca caagctacgt acagcgtatt
     1201 ggctacgttg tgccctcaac gcgggcagaa cagcagacag catctccctc atccggggaa
     1261 gcgcctgccg tcccgtccga caacactgtt tacccggata gcgtcatacg cggcgtaatt
     1321 ccggcacccg acaccacccc gaccagcatc gctgcttatc cgccacatgt cgtgcgcggc
     1381 ggcctcgctg tgtcttctga ttaaggaaga gatcatgact ttacgtacta tcaaaaccgc
     1441 tgcaacccgc ctgattgccg cattttccgc cctgtctgct gttccagcaa tggcggccag
     1501 cgctgaccag gcatttcaga aaggcagtga cgccattaac tgggtcttcc tcggcctaac
     1561 tggtatgtca gtcgtcacta tcagctgtgc ggtggccttt atcgcctaca agatgcttgt
     1621 tgacggcaag gcctggcacg atctccagcg cgtgttctgg gcgggtgtcc tgctcgccgg
     1681 ggcgtccggg ttcggtgcct acttcgccgc ctaaggaggc agcatgacca tcctcaacaa
     1741 ggcgctgacc cgaccggcgg cgctgtgggg ggtcccgctt gtccccctgc tgttcaccct
     1801 gtgcatcctt accctggcgg ccgtctggtc acgtttttac tggcttctcc tgcttctcat
     1861 acctgccttt atcgagctga gacggctggc aaaagaggac attcatttct ttgacctgaa
     1921 gttgctggcg tttaaaacgc gtggcaaccg cgcggcgacc cggcatttcg ggacaaaggc
     1981 gcttgtcgca aaccagtacg acgccataga cgttacggag tttataaaac atatgcggct
     2041 caacgagagt atcccgctgt cccgctatct cccctggtct tcccacctgc acgagcaggt
     2101 ggtgaagaac aaaaaaggtg actttgtcag cacctgggag atcggcggta tcatttttga
     2161 atgcgaggat gaacattacc tcgccaccct ggacatgcag ctcaacaacg ttatccgctc
     2221 ctttgagggg ctacccgtta ccttctatac ccatctggtg agcgacgaat ttaccgacac
     2281 gcttgaatgt gaatccggga ttccgttttc agatgaaatc gcgcgtctgt tttacgcctc
     2341 ccgcgagggc aaacctttct accagcgtcg cctcttcttc accgtctgct tcaatccgca
     2401 caccggcatt gagcgggcga ccatgaaaca gaagtccctg ggcgaacgta aaaccctgct
     2461 cgacaatgcc ctgcaggtga tgcaggagca ccggcaagcg attggcacgg cgctgtcacg
     2521 ctacgcggcg cggccgcttg gcacttatga ggagaacggg cacgtgttct cctcccagct
     2581 ggcgttttac aactttctga tcaccggcag gtggcagaaa gtccgcgtca cccgcacgcc
     2641 gttttatgac gtgctgggcg gcacggatat tttcccttcc accgacattg gccaggtcag
     2701 ccacaccgac ggccagagct actttcgcgg gctggaaatc aaggattaca gcactgaaac
     2761 catcaccggg ctgtatgact cgcttcttta tgcccgctgc cggtacgtgt tcacgcagtc
     2821 ctatacctgc atgggtaagg atgaagcgcg gggctgcatc cgcaccatcg agaaacgcct
     2881 gaagtctgcc gaggatgatg cgatctccca gcaggaagac ctgaaggtcg cccgcgacat
     2941 gcttcagtcc ggcattattt cattcgggaa atatcaccag acgcttattg tcagcgcccc
     3001 aaccagcgag caggttatcc gggacaccaa caccctggcg gataccctga aggatctcgg
     3061 tttcatcatg accctgtccc gcgagtcgct gtcggcggcg tatctcgcgc agttgccggg
     3121 tgtgtatcac ctgcgcccgc gtctggtgcc ggtgagcagc ctcaactacg ccgaaatggt
     3181 gtcgtttcac aactttcacc cgcacaagcg ggacgggaat gcctgggggg aggcgattgc
     3241 cgttgttccg accccgtccg ggggatgtca gtacgtgaat ctgcacaaca gccaggccga
     3301 tgttgacgag ttcagcgaga agccgccggg taacaccatg ctgctcggca gtaccgggac
     3361 ggggaaatcg accctgctga tgatgttcca gcatctgatg cagaaatacc ggcgtcctga
     3421 gtcgtttgcg ccgggggcga cgaaaaagcg cttcacctcg gtggtctttg ataaagaccg
     3481 ttcggcggaa atgtcgatac gcctccaggg cggctcgtac ttccgtttcc gttcaggcga
     3541 gaagaccggc cttaacccgt ttgcgctcgc tccaacgaaa cgtaaccgca acttcctcaa
     3601 gaagctgatg cgaatgatgt gcacaaggga cggcaaaccg ctcgacccgc gcgatgaaga
     3661 acgcctcagc gaggcagtgg acaccatcat gctggactac ccgcctgagc ttcgtcgcaa
     3721 cggcatcacc cgcatgctgg aagtgcggca tgaagccccg acgaaggacg cacggatgaa
     3781 cgggctgcgc atccgtctcc agcagtgggc gcagggcggg gagtttggct gggtgtttga
     3841 taatgacgtg gacacctttg atatcgacaa tattgataat ttcggcatcg acggcacgga
     3901 gttcctgaac gacaaagacg tgcgcagccc gctcaccttc tacctgctct accgcgtgac
     3961 cagcctcctc gatggccgcc gcctggtcat tttcatggat gaattctgga aatggcttca
     4021 ggacccggag ttttcaaaat tcgccctcga catgctgaag gtcattcgaa agctcaacgg
     4081 cattttcttc ccggccacgc agtcgccgga ggagatgtgc aagcacgaga tctcggcggc
     4141 cattatcgag cagtgtagta cccgcatctt catggcgaac cagcaggcga aggagtacga
     4201 ctacgtggac atgctgcggg tgccgaaagc ggtttacgac atcgtgacca ccctcgaccc
     4261 ctacgcccgc cagatggtga tcatgaaatc tccgctgcgg cgtggtgacg tgcgcccgta
     4321 tatcgcacaa atcaccctcg acctctccgg gctaggtcgc tacacgaagc tgctcagcgc
     4381 cagtgcggac aacctgaaaa tttttgacga gatctggcag gagggtatga agccggaaga
     4441 ctggaaagac gaaatgctga gacgtgccgt ttaatcacaa aaggataaca ccatgaaact
     4501 gaaaaccacc ctgacggcgc tgaccgtcgc cttgtgtctc tttcacgtcc cggctcaggc
     4561 cggtattccc gtttttgatg aagtagccaa cgccagcaac atccagcagt gggcggagaa
     4621 gctccagcag tggcagcaga cggttcaaca ttatcagtcg cagatgaacg cttataaaca
     4681 acagctggca acggccaccg gtattcggga cgtccaggca ttcctgaatc aggcaaaaaa
     4741 cctgacggct gacattaaaa atctgaaaaa cagggggatt tccttaaacg atctgttaac
     4801 cggctcaggc gggtcttatg ctggggaact aaacaggctg tatgacaaat acaaactgtt
     4861 cgacaactgc ccggagaacg ccagcgaggc gtaccgggat agctgcaaac agatgctcct
     4921 caatcaggcg gtgtccgtcg aggacacttc tgacatccag gaaaaaattt ccgacacgct
     4981 gagcgatatt agtaatctcg ccagtcgcat cgagaatgcg aaggacagta aagaatcgca
     5041 ggatctggcg aacgtagtgt ccgcgcggag tatccagctg aacgccctga ccactcaatg
     5101 ggaaatgtcg gtcaaacaaa gtgagatgcg caaccagctg cttgccgaac aacgccgaaa
     5161 ggcgcaggcc gagcaaatgc gcacagctcc aatcgcagac cttaactaac aggtgacccc
     5221 atgaaactga agacactgac ctttattgtt ctggcctcag tggccgcgct tactggctgc
     5281 gaggataaaa cctccgtaga ctggtatatg gcacaccatg acgacatgct ggcgaaatac
     5341 agggagtgca tttttaatca taattttgtg cccaccgatt gccagaatgc acgctccgcc
     5401 cttcaccgtg aaaaagctaa acctgatgtt gccgaaggat ataagcaaat cctgaaagac
     5461 cttgataaag ctcgcgtgca agagcctatt gctgacctga attagcgaca ggagaacgca
     5521 ccatgagtgg aatattcgtc ggattaaaca gccacatcat ggaggggttt gacagcgtgc
     5581 tcaccagcgt ggccgccgat acgggggggt ggatggcaca cctgttctcc atcgcggtgc
     5641 tctcgctgta catcacgtgg cgcgcctacc agacgatggc aggcaagcta gtccgcccct
     5701 tcgaggagat cgtgtgggac gtgagccgga tgctcatcat catgattttc gtcaccaacg
     5761 ccggaggcta tctcgatctg gtttcggatg ccatcaccgg cctgcgggac ggattcagcg
     5821 gcgacgcgtc gatctgggcg cggctggata ccgtctggga aaaggctcaa gcacttgggg
     5881 agaagcttta ctcttatgat gattcaacct atgtcgctct aaaaggcact ctggcgcagt
     5941 ttctggtctg ggcaggcgtc atgttactgg taggcatcac ggccatcatt aacctgatag
     6001 ccgaactggt gatgctgctg atgctggcga ccgccccgat ctttatctac tgcctgatgt
     6061 ggtcgtggtt ccgggatatg ttcagcaact ggctcagaat tattctgggc tgcatcctta
     6121 catccctgtt cagcggcatc gccctgacgg tggtaatgga ttacctggac tacatcctgg
     6181 cgggcgccac aaaagcatcc gatgccaact acctgactct ggccgttcag gtcttcctcg
     6241 gcacggcggg tgccgccgcc gtgatggtcc tgacctataa gctggccgag gcgcttgccg
     6301 gtgcatccgc acagggtgcc gctcaggcac ttgccgcgtc gggagtaggt tcagcccgtc
     6361 aatccgcctc ctccgcagcc ggtgcggtgg gggataaagc ctcacagaac cttaacagca
     6421 ccggcagcgc aacggcctcg tctgcctcca cggcctcccg cgccaccggc gatgcgcgca
     6481 gccgcagccg cgccgccgtt cagcggatgc aggaacgcta ccagagataa cccagtctga
     6541 ctgaactgcg ggacgccgca acggcgtcct gcggcttcgt cacgtcttta ccacaggaaa
     6601 aacaccatga aatacggcat cacgctgctg atgctgtgcc tgcttgcagg ctgcgcacag
     6661 cgaaacaccc aacttccgcc cgtgtcgggc aacccggagc cggtcaattc cccggccatt
     6721 attcaggagt taacccccca tgtctgaact taccgccaaa gccctgagcg actcgcgctc
     6781 gtttgagaac gccattcttg agcgggatat gcgcatgaaa aaggcgggct ggatcgtggc
     6841 ggcgctcgcc ggtctgctgc tcgtcttcgc ccttgccgcc cttatcctgc tgctaccgct
     6901 caaaaccacc accgtggagc tgtacacgct cgaccgtcag accgggcgca tcgagcgcgt
     6961 caccaccgtc ggcgaggaca agctggacac ctccgaggtg ctcaatctct cgcagactgc
     7021 gtcctacgtg aagcgccgcg aggggtacaa ctatttcgcc cttcagcagg actacagaga
     7081 gacgcagatg ttcaacagcg acgaggtcaa caaggaatac ctcgactggt ttaacagccc
     7141 ggacgcgccg gacgctgtgt ttcagcaggc ggcctatgta gtcacggtgg aagtcctctc
     7201 caacgtaccg tcagagggca ccggctccag catggtggag atgctgcgca tcaagcgcac
     7261 cattcgaaag gtcaaggaca acaccgaaac gtatgattac tggacggtgc gcatgaccta
     7321 ccgctatttc ccgcagaaga aaatgacggc ctccgagcgc gaggtgaacc cgttcggctt
     7381 catcgtcacc agttaccagc ggtttaagga gaaaagcgat gaataaggta ctgatgtgtg
     7441 ccctcctgct gaccgcagcg tcagcatggg gggcggcgac accgcgcggc agcgcctatg
     7501 acagccgcat gcagaacatc ccctacaaca gccagaacac gacagtgatt aacacgcggg
     7561 cgggttacat cacgacgctg gtttttgacg ccgacgagga ggtgatcacc gccaccgctg
     7621 gttttccgca gggctggacg atcacacccg accgtaaccg cgtggggatt agccccgcgc
     7681 cggtgaagca gccggtaacg gatgcggacg gcaaaaccgt ccagaagatt ttcaccccca
     7741 cgcagcagga ctggcagacc aacctgtttg tgaccaccac aaagcgcgac tacagcctga
     7801 tgctgaacgt gatcgataac gatcgcgagg cgggaaatct ggcctttgtg gtgcgcttca
     7861 gctaccccga cgatacccgc aggcagaacg acgcagcccg cctcgcccgt cagcaggagc
     7921 ttcaggccgc gcaggagaaa gaacgcattc agtttgccct gaagaaatcc actgcgccgc
     7981 gcaactggaa ctacacccgc agggtggcgg caggttcgga gtcgatcacc ccggattttg
     8041 cgtatgacga cggccggttt acctatctcg gcttttcacc gcagaagctg atcccgtcgc
     8101 cgaccgtcat cgtcggcggc agcgagcagg tgatcacccc gaccttcagc accgagggta
     8161 actaccgggt gatggtactg cgctctcttt ctccacgctt tgtgctgcgc tacggcagcc
     8221 aggtcgtggg gattgaaaac ggcagttacg ggaaagtcac cgtggcgaac ggcagcaccg
     8281 tctcaccgga tgtgaaactg gagagcaaat gatgaacgaa caggaagaca ttaaaaccgc
     8341 cgccgaactg gaggcggaag cccgcgagcg ggcgcgggca gagattgacg gcgataaacc
     8401 cgccgcaggc gcggtgccac ccggacagcc acaggtggtg cagcacgcga agcgccgcag
     8461 ccgccgctcg ctcgtgctgg ccgccatcag cctggtgtta ctggtcatta tcgccaccgg
     8521 cggggattac ctgatggcga aactgaaggg tgacggcagc ggaaaaagcg cgagccatga
     8581 accggaatcg cacgccacct cccccgcccg cgagcgcaat aacctcggac gggatctgaa
     8641 cccgctggga cagctggcag acacaaccgg ccaggagaag actactccga ctgataccgc
     8701 cgcgccgccg caggcatcac cgccccccct gacgtttgac aaggtcagcg ccctggcaga
     8761 cagcgcccca accactgcaa gcagcgcccc gaaaagcacg tccagcagta acgtatcacc
     8821 tgcgggcagc gcgtccgcgc caggctctgc aacacccgcc gggcagtcgc aggataacgg
     8881 gctggcgcgc attaccggcg tgaagcggct cggcctcgat ccggatttgt acattccggc
     8941 cgaccggcat atcccctgcg cgatgatgca gcgctttgta tcggacgtgg gcgggcgctt
     9001 taactgcctt atcagcgagg acatttacag cgccagcaac gccgtgcgcc ttatcccggc
     9061 aggcactgag gcgcgcggcc tgtaccgcac cggcacgctg aaaaacgggc aggggcgtct
     9121 gttcctcgcc atcaccgagc tgcgcacgcc ggaacccggc cgcctggtta ttccgatggt
     9181 tgacagccag gccgtggggg cgcttggcga gaacggcgtc gcgggctgga tagacaatca
     9241 ctggctggag cggataggta ataccctcct gctcggcacg gtacaggact ttgccgctgc
     9301 ggcctccggc tcgtcgccgg gcaaggatcg caacacggac tacacggaga acacccgcgc
     9361 ggccacggcg gaaatggcaa aaacgctcct ggaaaacagc atcaacatcc cgccgacgat
     9421 gtacctcaac cagggtgacg tgattggcct ggtcacgggg gcggacattg atttttcaga
     9481 cgtctacaga ctgcgcatga ggtaagcgat ggacgataaa ctgactgata ccccgtcaga
     9541 gggcaacgtc gcgctgtcac acctgcgcga tctgttgttc ggggactacc tgagccggga
     9601 ggggctgacg gaaattgcca tcaaccgccc cggagagctg cacacaaaaa tcaacggggc
     9661 gtgggaaatg cacgcctcgc cggtcacgct gcgccagtgc tcgtcctttg ccagggcgct
     9721 ggccgtgtgg aacaacgaca ccattgatga aacgtcgcct atcctctcgg ccacgctccc
     9781 tacaggagaa cgctgccaga tagtggtgcc cccggcctgt gagcgtaaca ccatctcaat
     9841 caccctgcgt aagccggact acttccagcg cacccatcag tcgtatatcg atgccgggtt
     9901 ttatgaccgg gtgcagggta cagaaaaggt ggaatcaaaa gacggggagc tgagccgcat
     9961 atacctgtcc gggaatatcc cgctttttat ggaaaagtgc gtggagtacg gcaaaacgct
    10021 ctttgtggtg ggggaaacag gctctgggaa aaccacctac atgaagatgc tgctgcacta
    10081 cattcccgag acgctgcgcc ttatcaccat cgaggacaac ccggagctga agttttacag
    10141 ccagaaaaac tacgttcatc tgttctaccc ggcggacgcc ggagagggcg caatagtcac
    10201 ctctgatcgc ctggtgcgcg cgatttaccg tatgaacccc gaccgccccc tgctcgccga
    10261 ggtgcgtggc cgcgaggcgt gggacgcact caagctgatt gagtccgggc acgaggggtt
    10321 tatgtcctcc ctgcacgcgg gcagcccgcg cgagtgtatc tccgggctga ttgaccgctg
    10381 ctacgagcat ccggcgtgcc ggggcatgcc ggttgacgtg ctgctgcgca aggtactgca
    10441 ctgcgtcgac gtcattgtga gcgtggacgt tcacggcaac gtgcgccgca tgagcgacgt
    10501 ctattttaaa cccctgcaca tgcaggctat gaaggagagt ttcaatgatg ctgcctaaac
    10561 tgaccggcct gacggcgatc gtcgtcctcc tggcgggctg ttcttcgcca ccggagcctg
    10621 cggccgttga ctggtcacaa ccggcgcagc cggtaacgac cacccggccg gtatgggaac
    10681 ccaaccgtct tatcgtggcc tcgccggtga cggaagggca ctggtcgcag gtgcttacgc
    10741 gttttgaacc cggcgggatt tacccggccg cacagtggta cgcggttgcg cacgcctcgc
    10801 gcgcggtagt aaatgccccg gacggccagc gcttctttgc ggccaaaacc tggctccgcg
    10861 agggcggcta caagggtgtg gtggagtttc gcccccgctc cggctgcctg acctgcgaca
    10921 cctcagaaat cgt
//
LOCUS       DAZPEB010000136        10903 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZPEB010000136 DAZPEB010000000
VERSION     DAZPEB010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10903
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.138"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..184
                     /locus_tag="ACHURJ_003033"
     CDS             <1..184
                     /locus_tag="ACHURJ_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649797.1"
                     /note="interacts RssB preventing the degradation of the
                     stress sigma factor RpoS; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFR1281891.1"
                     /translation="KLGIILQEKEYPITIYNIAPFNKNLWSLLKSSQECPPGESKITN
                     KCLHNSCIIKICSICY"
     gene            complement(252..1502)
                     /gene="icd"
                     /locus_tag="ACHURJ_003034"
     CDS             complement(252..1502)
                     /gene="icd"
                     /locus_tag="ACHURJ_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFR1281892.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGEAIIENM"
     gene            1674..2327
                     /gene="rluE"
                     /locus_tag="ACHURJ_003035"
     CDS             1674..2327
                     /gene="rluE"
                     /locus_tag="ACHURJ_003035"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309634.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFR1281893.1"
                     /translation="MRQFIISENTMQKTSFRNHLVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYTLDNLANGEWREATE"
     gene            2337..2798
                     /gene="nudJ"
                     /locus_tag="ACHURJ_003036"
     CDS             2337..2798
                     /gene="nudJ"
                     /locus_tag="ACHURJ_003036"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFR1281894.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILKASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2852..3958
                     /gene="mnmA"
                     /locus_tag="ACHURJ_003037"
                     /gene_synonym="trmU"
     CDS             2852..3958
                     /gene="mnmA"
                     /locus_tag="ACHURJ_003037"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFR1281895.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCSVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3994..4635
                     /gene="hflD"
                     /locus_tag="ACHURJ_003038"
     CDS             3994..4635
                     /gene="hflD"
                     /locus_tag="ACHURJ_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFR1281896.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVPAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4639..6009
                     /gene="purB"
                     /locus_tag="ACHURJ_003039"
     CDS             4639..6009
                     /gene="purB"
                     /locus_tag="ACHURJ_003039"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFR1281897.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6177..6848
                     /gene="phoP"
                     /locus_tag="ACHURJ_003040"
     CDS             6177..6848
                     /gene="phoP"
                     /locus_tag="ACHURJ_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFR1281898.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6848..8308
                     /gene="phoQ"
                     /locus_tag="ACHURJ_003041"
     CDS             6848..8308
                     /gene="phoQ"
                     /locus_tag="ACHURJ_003041"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFR1281899.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            8384..9505
                     /gene="roxA"
                     /locus_tag="ACHURJ_003042"
                     /gene_synonym="ycfD"
     CDS             8384..9505
                     /gene="roxA"
                     /locus_tag="ACHURJ_003042"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFR1281900.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(9554..10780)
                     /gene="pepT"
                     /locus_tag="ACHURJ_003043"
     CDS             complement(9554..10780)
                     /gene="pepT"
                     /locus_tag="ACHURJ_003043"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFR1281901.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLAGMKGTVERADMH
                     YIIRDFDRKQFEARKCKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
BASE COUNT         2696 a         2788 c         2726 g         2693 t
ORIGIN      
        1 taagcttggt attatccttc aggaaaaaga atatcctatt actatttata acattgcacc
       61 attcaacaag aatttatgga gtttactcaa aagcagtcaa gagtgtcctc caggagaaag
      121 caaaataaca aataaatgtt tacataatag ttgcattata aaaatatgct cgatatgcta
      181 ttaataatat ccacataaat aaaacaacgg gcgtgttata cgcccgtttc aatatttaac
      241 tcatggagag attacatgtt ttcgatgatc gcttcaccaa actctgaaca tttcagcagc
      301 ttagcgcctt ccatcagacg ttcaaagtca taagttacgg ttttcgcgtt gatagcacct
      361 tccatacctt taacaattaa atcagccgct tcagtccacc ccatatggcg cagcatcatc
      421 tcagcggaga ggatgataga gcctggattt actttgtcct gaccggcgta tttcggcgca
      481 gtaccgtggg tggcttcaaa cagggcgcat tcgtcaccga tgtttgctcc aggagcgata
      541 ccgataccgc caacctgcgc tgccagggcg tcagaaatgt agtcaccgtt caggttcata
      601 caggcgataa catcatattc agccggacgc agcaggatct gttgcaggaa tgcatcagca
      661 atcacgtctt taatgacgat ctctttgccg gtgttcgggt ttttaacttt cagccacggg
      721 ccgccgtcga tcagttcacc gccaaactct tcacgcgcca gctggtagcc ccagtcttta
      781 aacgcgcctt cggtgaactt catgatgttg cctttgtgca ccagagtcac agagtcacga
      841 tcgttagcaa ttgcgtattc gatcgctgca cgaaccagac gtttggtgcc ttcttccgaa
      901 cacggcttaa taccgatacc acaatgttcc gggaagcgaa ttttcttcac ccccatctct
      961 tcacgcagga atttaatcac tttctcggcg tcggcagagt ctgctttcca ttcgataccc
     1021 gcataaatgt cttccgagtt ttcacggaag ataaccatat cggtcagttc agggtgttta
     1081 accgggcttg gagtgccctg atagtaacgt accggacgca ggcagatgta gagatccagt
     1141 tcctggcgca gggcaacgtt cagagagcga ataccgccac caacaggagt ggtcagcgga
     1201 cctttaatgg caacgcgata ttcacgaatc agatcaaggg tttcagcagg cagccagaca
     1261 tcctgaccat aaacctgtgt ggatttttca ccggtgtaaa tttccatcca ggagatttta
     1321 cgctcgcctt tataggcttt ctcgactgca gcgtcgacca ctttcagcat ggctggggtt
     1381 acatctacac cgattccatc accttcaatg taagggataa tcggattttc aggaacgttg
     1441 agtttgccgt tttgcagggt gatcttcttg ccttgtgccg gaacaactac tttactttcc
     1501 attcacctct ccttcgagcg ctactggttt gctcgtctgc caccacgttg catatgcgtt
     1561 tgcgtcctgc gatacggatg ctttagagca attttttgtt aatgatttgt aattggcttg
     1621 tcaatactac cctattgttt gccgtcatga aagatgcgcg ttattaggct ataatgcggc
     1681 aattcataat ctctgaaaat accatgcaaa aaacttcttt tagaaatcac ctggttaagc
     1741 gattcagctc gcaacgttct accaggcgta aacctgaaaa ccagcccacg cgtgtgatcc
     1801 tgttcaataa accctacgat gttcttccgc agttcaccga tgaagccgga cgcaaaacat
     1861 taaaagaatt catcccggtt cagggcgttt atgcagcagg tcgtcttgac cgcgatagcg
     1921 aagggttact ggtgctgacc aataacggcg cgttgcaggc gcgtttaacc cagccgggca
     1981 aacgcaccgg gaaaatctat tatgtgcagg tggaaggtat tcccacacaa gacgcacttg
     2041 aagccttgcg caatggcgta accttaaatg atggccctac cctgcccgcc ggcgcggagc
     2101 tggttgacga accagcgtgg ttatggccgc ggaatccacc aattcgtgaa cgcaaaagta
     2161 ttcccaccag ctggctgaag atcaccttat atgaaggacg taatcgccag gtgcgccgca
     2221 tgaccgccca tgttggtttc cccacgctgc gactgattcg ttatgcgatg ggtgattaca
     2281 ctttggataa ccttgccaat ggcgaatggc gagaagcaac agaataagga tgcacaatgt
     2341 ttaaaccaca cgttaccgtt gcttgcgtag tgcacgcaga gggtaaattt ttagtcgttg
     2401 aagagacgat taatggtaaa gcgttatgga accaacctgc cgggcatctg gaagccgatg
     2461 aaaccttagt ggaagctgcc gcccgtgagc tatgggaaga aaccggcatc agcgcgcaac
     2521 cgcaacactt tattcgtatg catcagtgga ttgcgccaga taaaacgccg tttttgcgtt
     2581 tcctctttgc cattgagctt gagcaaatat gcccgaccca gcctcatgac agcgatatcg
     2641 actgctgtcg ttgggtcagc gccgaagaaa ttttaaaggc gtcaaatctt cgttcgccgc
     2701 tggtggcgga aagtatccgt tgttatcaaa gcgggcaacg ctatccgctg gagatgattg
     2761 gcgattttaa ctggcctttt acaaagggtg tcatctaaaa ggctgcgtga tagaatacgc
     2821 cgccttgaag ttcaatgtcg tgagtgatcc aatgtctgaa accgcaaaaa aagtaatcgt
     2881 cggcatgtcc ggcggtgtcg attcctccgt ttctgcctgg ctgttgcaac aacagggata
     2941 tcaggttgaa ggcctgttta tgaagaactg ggaagaagac gacggtgagg aatattgcac
     3001 agcggcagcg gatctggctg atgcccaagc tgtctgcgac aagctcggca ttgaactgca
     3061 caccgttaac tttgctgccg agtactggga caacgtcttc gaactgttcc ttgccgaata
     3121 taaagccggt cgcacgccga atccggatat tctgtgcaac aaagagatca aatttaaagc
     3181 tttcctcgaa tttgccgccg aagatttagg tgccgattat atcgctaccg gtcattacgt
     3241 acgtcgggcc gatgtcgatg gcaagagccg cctgctgcgt ggtctggaca gcaataaaga
     3301 ccagagctac ttcctttata cgctcagcca tgagcagatt gcgcaaagcc tgttcccggt
     3361 cggcgaactg gaaaaaccgc aggtgcgtaa gattgctgaa gatcttggtc tggtcaccgc
     3421 gaagaaaaaa gactctaccg gcatttgctt cattggcgaa cgtaaattcc gcgagttcct
     3481 gggccgttat ctcccggcgc aaccgggcaa aatcattacc gtcgatggcg atgaaattgg
     3541 tgagcaccag gggctgatgt atcacactct cggtcagcgt aaaggtctgg gtatcggtgg
     3601 caccaaagaa ggcaccgaag aaccgtggta tgtggtggac aaagacgtcg aaaacaacat
     3661 tctggttgtt gcgcagggcc atgaacaccc gcggctgatg tctgtcgggt tgattgccca
     3721 gcagttgcac tgggtcgatc gcgaaccatt caccggtact atgcgttgct cggtaaaaac
     3781 ccgctatcgc cagaccgata tcccttgcac cgtcaaggcg ctggacgatg atcgcattga
     3841 agtgattttc gatgaaccgg ttgccgccgt gacgccgggc cagtctgccg tcttctataa
     3901 cggtgaagtg tgcctcggtg gcggtattat tgagcagcgt ctgccgctgc cggtctgatt
     3961 attatcttta cttaaaacaa ggaagcagtg aacgtggcaa agaattacta tgacatcacc
     4021 ctcgccctgg ccggtatttg tcagtcggca cgcctggtgc aacaactcgc tcaccagggg
     4081 cattgtgatg ccgatgcgct acacgtctca ctcaacagta ttattgatat gaaccccagc
     4141 tcgacgctgg cggtttttgg cggtagcgaa gccaacctgc gcgtcgggct ggaaaccctg
     4201 ctcggcgtgc tcaatgccag cagtcgccag ggcttaaacg ccgaattaac ccgctacaca
     4261 ctcagcttga tggtgcttga gcgcaaactc tcctcagcga aaggcgcgct cgacactctg
     4321 ggcaaccgga tcaacggcct gcaacgccag ctcgaacact tcgatttaca gtccgaaacg
     4381 ctgatgagcg cgatggctgc tatctatgtt gatgtgatca gcccgcttgg cccgcgcatt
     4441 caggtcaccg gttcccctgc tgtactgcaa agcccacaag tgccggcgaa agttcgcgca
     4501 accctgctgg caggcattcg cgccgccgtg ctctggcacc aggtcggcgg cggacgtctg
     4561 caactgatgt tttctcgtaa tcgcctgacc actcaggcaa aacaaattct tgctcattta
     4621 accccggagt tgtgatctat ggaattatcc tcactgaccg ccgtttcccc tgtcgatgga
     4681 cgctacggcg ataaagtcag cgcgctgcgc gggattttca gcgaatatgg tttgctgaaa
     4741 ttccgtgtac aagttgaagt acgttggctg caaaaactgg ccgcgcacgc agcgatcaag
     4801 gaagttcctg cttttgctgc cgacgcaatc ggttaccttg atgcaattgt cgccaatttc
     4861 agcgaagaag atgccgcacg catcaaaacc atcgagcgta ccactaacca cgacgttaaa
     4921 gcggttgagt atttcctgaa agaaaaagtg gcggagatcc cggaactgca cgcggtttct
     4981 gaattcatcc actttgcctg tacttcggaa gatatcaata acctctccca cgcattaatg
     5041 ctgaaaaccg cgcgtgatga agtgatcctg ccgtactggc gtcaactgat tgatggcatt
     5101 aaagatctcg ccgttcagta ccgcgatatc ccgctgctgt ctcgtaccca cggtcagcca
     5161 gccacgccgt caaccatcgg taaagagatg gcaaacgtcg cctaccgtat ggagcgccag
     5221 taccgccagc ttaaccaggt ggagatcctc ggcaaaatca acggcgcggt cggtaactat
     5281 aacgcccaca tcgccgctta cccggaagtt gactggcatc agttcagcga agagttcgtc
     5341 acctcgctgg gtattcagtg gaacccgtac actacccaga ttgaaccgca cgactacatt
     5401 gccgaactgt ttgattgcgt tgcgcgcttc aacaccattc tgatcgactt tgaccgtgac
     5461 gtctggggtt atatcgccct taaccacttc aaacagaaaa ccattgctgg tgagattggt
     5521 tcttccacca tgccgcataa agttaacccg atcgacttcg agaactccga agggaatctg
     5581 ggcctttcca acgcggtatt acagcatctg gcaagcaaac tgccggtttc ccgctggcag
     5641 cgtgacctga ccgactccac cgtgctgcgt aacctcggcg tgggtatcgg ttatgcgctg
     5701 attgcgtatc aatccaccct gaaaggcgtg agcaaactgg aagtgaaccg tgaccatctg
     5761 ctggatgagc tggatcacaa ctgggaagtg ctggctgagc caatccagac agttatgcgt
     5821 cgctatggca tcgaaaaacc gtacgagaag ctgaaagagc tgactcgtgg taagcgcgtt
     5881 gacgccgaag gcatgaagca gtttatcgac ggtctggcgt tgccagaaga agagaaagcc
     5941 cgcctgaaag cgatgacgcc ggcaaactac attggtcgcg ccatcaccat ggttgatgag
     6001 ctgaaataaa ctcgtatcag tgccggatgg cgatgctgtc cggcctgctt attaagatta
     6061 tccgcttttt attttttcac tttacctccc ctccccgctg gtttatttaa tgtttacccc
     6121 cataaccaca taatcgcgtt acactatttt aataattaag acagggagaa ataaaaatgc
     6181 gcgtactggt tgttgaagac aatgcgttgt tacgtcacca ccttaaagtt cagattcagg
     6241 atgctggtca tcaggtcgat gatgcagaag atgccaaaga agccgattat tatctcaatg
     6301 aacatttacc ggatattgcg attgtcgatc tcggattgcc agacgaggac ggtctgtcac
     6361 tgattcgccg ctggcgtagc aatgatgttt cactgccgat tctggtatta accgcccgtg
     6421 aaagctggca ggacaaagtc gaagtattaa gtgctggtgc tgatgattac gtgactaaac
     6481 catttcatat tgaagaggtg atggcgcgaa tgcaggcatt aatgcggcgt aatagcggtc
     6541 tggcttcaca ggtcatttcg ctaccaccgt ttcaggttga tctctctcgc cgtgaattat
     6601 ctattaatga cgaagtgatc aaactgaccg cgttcgaata caccattatg gaaacgttga
     6661 tacgcaataa tggcaaagtg gtgagcaaag attcgttaat gctccaactc tatccggatg
     6721 cggagctgcg ggaaagccat accattgatg tactgatggg acgtctgcgc aaaaaaattc
     6781 aggcacaata tccgcaagaa gtgattacta ccgttcgcgg ccagggctat ttgttcgaat
     6841 tgcgctgatg aaaaaattac tgcgtctttt tttcccgctc tcgctgcggg tacgttttct
     6901 gttggcaacg gctgcggtag tactggtgct ttcgcttgcc tacggaatgg tcgcgctgat
     6961 cggttatagc gtcagtttcg ataaaactac gtttcggctg ttacgtggcg agagcaatct
     7021 gttctatacc cttgcgaagt gggaaaacaa taagttgcat gtcgagttac ccgaaaatat
     7081 cgacaagcaa agccccacca tgacgctaat ttatgatgag aacgggcagc ttttatgggc
     7141 gcaacgtgac gtgccctggc tgatgaagat gatccagcct gactggctga aatcgaatgg
     7201 ttttcatgaa attgaagcgg atgttaacga taccagcctc ttgctgagtg gagatcattc
     7261 gatacagcaa cagttgcagg aagtgcgaga agatgatgac gacgcggaga tgacccactc
     7321 ggtggcagta aacgtctacc cggcaacatc gcggatgcca aaattaacca ttgtggtggt
     7381 ggataccatt ccggtggagc taaaaagttc ctatatggtc tggagctggt ttatctatgt
     7441 gctctcagcc aatctgctgt tagtgatccc gctgctgtgg gtcgccgcct ggtggagttt
     7501 acgccccatc gaagccctgg caaaagaagt ccgcgaactg gaagaacata accgcgaatt
     7561 gctcaatcca gccacaacgc gagaactgac cagtctggta cgaaacctga accgattgtt
     7621 aaaaagtgaa cgcgaacgtt acgacaaata ccgtacgacg ctcaccgacc tgacccatag
     7681 tctgaaaacg ccactggcgg tgctgcaaag tacgctgcgt tctctgcgta gtgaaaagat
     7741 gagcgtcagt gatgctgagc cggtaatgct ggagcaaatc agccgcattt cacagcaaat
     7801 tggctactac ctgcatcgtg ccagtatgcg cggcgggaca ttgctcagcc gcgagctgca
     7861 tccggtcgcc ccactgctgg acaatctcac ctcagcgctg aacaaagtgt atcaacgcaa
     7921 aggggtcaat atctctctcg atatttcgcc agagatcagc tttgtcggtg agcagaacga
     7981 ttttgtcgag gtgatgggca acgtgctgga taatgcctgt aaatattgcc tcgagtttgt
     8041 cgaaatttct gcaaggcaaa ccgacgagca tctctatatt gtggtcgagg atgatggccc
     8101 cggtattcca ttaagcaagc gagaggtcat tttcgaccgt ggtcaacggg ttgatacttt
     8161 acgccctggg caaggtgtag ggctggcggt agcccgcgaa atcaccgagc aatatgaggg
     8221 taaaatcgtc gccggagaga gcatgctggg cggtgcgcgg atggaggtga tttttggtcg
     8281 ccagcattct gcgccgaaag atgaataaat atgtccatac ttcacgcatt acgttaagca
     8341 tccgttataa tcggttgcag ataccagcct gtggatgctt aacatggaat accaactcac
     8401 tcttaactgg cccgattttc ttgaacgtca ctggcagaaa cgcccggtgg tgttaaaacg
     8461 cggctttaat aattttattg acccgatctc tccagacgag ttggcgggtc tggcgatgga
     8521 aagcgaagtt gacagtcgac tggtcagtca ccaggatggc aaatggcagg tcagccacgg
     8581 cccgttcgaa agctacgatc atctcggtga aaccaactgg tcattactgg tacaggcagt
     8641 gaaccactgg catgagccga ccgccgcgct gatgcgaccg ttccgtgaac taccggactg
     8701 gcgtattgat gatctgatga tttctttttc tgtacctggc ggcggcgtcg gcccgcatct
     8761 cgatcagtac gacgtgttta tcattcaggg taccggacgt cgtcgctggc gagtgggcga
     8821 aaagctgcaa atgaaacagc actgcccaca tccggatctg ttacaggtcg atccgttcga
     8881 agccatcatc gatgaagagc tggagcctgg tgatattctt tatattccgc caggattccc
     8941 gcatgaaggc tacgcgctgg aaaatgcgat gaactattcc gtgggctttc gcgcgccaaa
     9001 tacgcgggaa ctgattagtg gatttgccga ttatgtgctg caacgtgaac tgggcggcaa
     9061 ctactacagc gatcctgatg ttccacctcg cgctcatcct gcggatgttc tgcctcaaga
     9121 gatggataaa ctgcgtgaga tgatgctcga attgatcaac cagccggaac actttaagca
     9181 atggtttggc gagtttatat cccagtcacg tcatgaactg gatatcgcgc cgccggaacc
     9241 gccttatcag ccagatgaaa tctacgatgc gctgaaacaa ggtgatgtgc tggtgcgcct
     9301 gggtggtctg cgcgtattgc gcattggcga cgacgtgtat gccaatggtg agaagatcga
     9361 ttccccgcac cgtccggcac tggatgcact cgccagcaac attgcgctga ctgcggagaa
     9421 ttttggcgat gcgctggaag atccgtcatt cctcgcgatg ctcgcggcgc tggtcaatag
     9481 cgggtattgg ttcttcgaag ggtaagtttg gatttaagcc ggatgcggca tcacatgccg
     9541 catccctttt cgcttacttc cgttgcgccg ttaactcggc aatacggacg atcacctgca
     9601 ccgctttttc cataccttcc agagtcacaa actcatgctt accatgatag ttgtaaccgc
     9661 cagtgaacaa gttcgggcac ggtaatccca taaacgacaa ctgcgcgccg tcggtaccgc
     9721 cgcggatcgg tttcagttcc ggttcaatat cgcagtcacg cattgcctgc tgggcgatat
     9781 cgagaatatg cggatgctca accactttct cgcgcatatt gtagtaactg tcttcaatca
     9841 ccagttcaat gtagcaatca ggatgtaacc ctttgcccac ttttttggcg atctccatca
     9901 ttttacattt acgcgcttca aactgtttac ggtcgaaatc acggatgatg tagtgcatat
     9961 cggcccgttc aacggtgcct ttcatgcccg ccaggtgata gaaaccttca tagccttctg
    10021 tcatttccgg gctttcatcc gccggaactt ccgcatgaat acgtgccgcc agcgacagcg
    10081 catttaccat cactcctttc gccgtgcccg gatgaacatt gttaccgaca attttgatat
    10141 tgaccgatgc ggcgttgaag ttttcaaact ccagttcgcc tacgccacca ccgtcaacag
    10201 tgtaagccca gcgggcatcg aaggcatcaa catcaaaatg tttcgcccct ttgcccactt
    10261 cttcatccgg ggtaaaggcg acgcgaatat caccatgcgg aatgtttttc tgttgcaata
    10321 ccgccagcgc ggtcatgatt tctgcaatac ctgctttgtc atcggcacct aacaaggttt
    10381 taccatcggt ggtaatcaat gtctgaccca gtagctgatg cagcaccggg aacataaccg
    10441 gtgataaaac ttcatcgccg atacccagcg caatatcgcc accgcgatag ttttcaacaa
    10501 tttgcggatt aacatttttg ccgctgcaat ccggtgaggt atccacatga gaaataaagc
    10561 caatcgccgg gatatcgcca gggacgttag ccggtaacgt cgccatcaaa gtgcccttct
    10621 cacttaaggt cacattgata agccccattt cttcgagctg ctctttcagc agatgcaata
    10681 acttccattg gccttccgtg ctgggaacct gtctcacccc tgcttttgat tgggtatcca
    10741 gagacacgta gttcaaaaat cgctcaagta gtttatccat gtagtcaccc tcactttttg
    10801 tgacaacatt attaagaagc aagaaaagac aaatattgcg tcaggtcact tttacccctg
    10861 caagcgggaa tatttatcag cattacctta atgaatataa agc
//
LOCUS       DAZPEB010000137        10856 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZPEB010000137 DAZPEB010000000
VERSION     DAZPEB010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10856
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.140"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..407)
                     /gene="dsbG"
                     /locus_tag="ACHURJ_003044"
     CDS             complement(<1..407)
                     /gene="dsbG"
                     /locus_tag="ACHURJ_003044"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFR1281902.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQA"
     gene            779..1342
                     /gene="ahpC"
                     /locus_tag="ACHURJ_003045"
     CDS             779..1342
                     /gene="ahpC"
                     /locus_tag="ACHURJ_003045"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFR1281903.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            1513..3078
                     /gene="ahpF"
                     /locus_tag="ACHURJ_003046"
     CDS             1513..3078
                     /gene="ahpF"
                     /locus_tag="ACHURJ_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFR1281904.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNLGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(3199..3627)
                     /gene="uspG"
                     /locus_tag="ACHURJ_003047"
     CDS             complement(3199..3627)
                     /gene="uspG"
                     /locus_tag="ACHURJ_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFR1281905.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLTQDDGVIHLLHVLPGSA
                     SLSLDRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            3848..5086
                     /locus_tag="ACHURJ_003048"
     CDS             3848..5086
                     /locus_tag="ACHURJ_003048"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HFR1281906.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLSFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEEAQKAVSGLVNAMPGGTI"
     gene            complement(5090..5179)
                     /gene="yldA"
                     /locus_tag="ACHURJ_003049"
     CDS             complement(5090..5179)
                     /gene="yldA"
                     /locus_tag="ACHURJ_003049"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFR1281907.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(5317..5727)
                     /gene="rnk"
                     /locus_tag="ACHURJ_003050"
     CDS             complement(5317..5727)
                     /gene="rnk"
                     /locus_tag="ACHURJ_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFR1281908.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(5957..6763)
                     /gene="rna"
                     /locus_tag="ACHURJ_003051"
     CDS             complement(5957..6763)
                     /gene="rna"
                     /locus_tag="ACHURJ_003051"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFR1281909.1"
                     /translation="MKEFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSRGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPSNCGKTFVIDKVGY"
     gene            complement(6874..8337)
                     /gene="citT"
                     /locus_tag="ACHURJ_003052"
     CDS             complement(6874..8337)
                     /gene="citT"
                     /locus_tag="ACHURJ_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFR1281910.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPNGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVRVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            complement(8457..9265)
                     /gene="citG"
                     /locus_tag="ACHURJ_003053"
                     /pseudo
     CDS             complement(8457..9265)
                     /gene="citG"
                     /locus_tag="ACHURJ_003053"
                     /inference="COORDINATES: protein motif:HMM:NF013991.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
     gene            complement(9240..9790)
                     /gene="citX"
                     /locus_tag="ACHURJ_003054"
                     /pseudo
     CDS             complement(9240..9790)
                     /gene="citX"
                     /locus_tag="ACHURJ_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
     gene            complement(9794..>10856)
                     /gene="citF"
                     /locus_tag="ACHURJ_003055"
     CDS             complement(9794..>10856)
                     /gene="citF"
                     /locus_tag="ACHURJ_003055"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFR1281911.1"
                     /translation="LNIDVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQV
                     VMLTEELLPYPHNPASIEQEQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAA
                     DVIVNSGYFKEGFSMQTGTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEK
                     GLIRKLLDVQSFDSHAAQSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEID
                     TQFNVNVLTGSDGVLRGASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGS
                     SVDILVTDHGIAVNPARPELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVV
                     AVVRYRDGSVIDVVHQVKE"
BASE COUNT         2760 a         2722 c         2840 g         2534 t
ORIGIN      
        1 gcctgctgcc agaactgttt acaatatggg cagaacggat cggcgaagac gtagacaatg
       61 accggcgcat cttttttacc gtcgaggagc cagtgggatt gttccatccg ttgccacatt
      121 tcgcgtccgg ctggtgcgta aatttctttt tcgataagtg tgttactcag gttttcacct
      181 ttctcgttgt acatgtaacc agagatagcg tgcttaccat ctggagtcag gtagatggtg
      241 acgcccatat cctgatactt tccgagataa cctttcattc ctccgggggc atcgaacgtt
      301 ttgatgattg taatgccctg tttttcaatc gcttttactg gagcaggaag ttcctctgcg
      361 aaggcgattg caggaagcag agccagtaaa agtatctttt ttaacattaa tttgtccttt
      421 tcagtcagtg caaaagtcga gtaaaaggca taacctatca ctgtcatagg taagagctta
      481 gatcaggtga ttgccctttg tttatgaggg tgttgtaatc catgtcgttg ttgcatttgt
      541 aagggcaacg cctcagcctg caggcaggcg ctgaagatac caaagggtag ttcagattac
      601 acggtcacct ggaaaggggg ccattttact ttttatcgcc gctggcggtg caaagttcac
      661 aaagttgtct tacgaaggtt gtaaggtaaa acttatcgat ttgataatgg aaacgcatta
      721 gccgaatcgg caaaaattgg ttaccttaca tctcatcgaa aacacggagg aagtatagat
      781 gtccttgatt aacaccaaaa ttaaaccttt taaaaaccag gcattcaaaa acggcgaatt
      841 catcgaaatc accgaaaaag ataccgaagg ccgctggagc gtcttcttct tctacccggc
      901 tgactttact ttcgtatgcc cgaccgaact gggtgacgtt gctgaccact acgaagaact
      961 gcagaaactg ggcgtagacg tatacgcagt atctaccgat actcacttca cccacaaagc
     1021 atggcacagc agctctgaaa ccatcgctaa aatcaaatat gcgatgatcg gcgacccgac
     1081 tggcgccctg acccgtaact tcgacaacat gcgtgaagat gaaggtctgg ctgaccgtgc
     1141 gaccttcgtt gttgacccgc agggtatcat ccaggcaatc gaagttaccg ctgaaggcat
     1201 tggccgtgac gcgtctgacc tgctgcgtaa aatcaaagca gcacagtacg tagcttctca
     1261 cccaggtgaa gtttgcccgg ctaaatggaa agaaggtgaa gcaactctgg ctccgtctct
     1321 ggacctggtt ggtaaaatct aaatttcctt cgtctttcgc gctaccaact ccggcgcgaa
     1381 atacttcgga aattcaccta attcttcggg tgctgcggca cccgattttc ttccccgcac
     1441 tatgatgcaa gctgcatcca ggtagccgta gaggccgctt gcatgatgat gtttaaagcc
     1501 caggagataa acatgctcga cacaaatatg aaaactcaac tcaaggctta ccttgagaaa
     1561 ttgaccaagc ctgttgagtt aattgccacg ctggatgaca gcgctaaatc ggcagaaatc
     1621 aaggaactgt tggctgaaat cgcagaactg tcagacaaag tcacctttaa agaagataac
     1681 agcttgccgg tgcgtaagcc gtctttcctg atcaccaacc taggttccaa ccaggggcca
     1741 cgttttgcag gctccccgct gggccacgag ttcacctcgc tggtactggc gttgctgtgg
     1801 accggtggtc atccgtcgaa agaagcgcag tctctgctgg agcagattcg ccatattgac
     1861 ggtgattttg aattcgaaac ctattactcg ctctcttgcc ataactgccc ggacgtggtg
     1921 caggcgctga acctgatgag cgtactgaac ccgcgcatca agcacactgc aattgacggc
     1981 ggcaccttcc agaacgaaat caccgatcgc aacgtgatgg gcgttccggc agtgttcgta
     2041 aacgggaaag agtttggtca gggccgcatg acgttgactg aaatcgttgc caaaattgat
     2101 acgggcgcgg aaaaacgtgc ggcagaagag ctgaacaagc gtgatgctta tgacgtatta
     2161 atcgtcggtt ccggcccggc gggtgcagcg gcagcaattt actccgcacg taaaggcatc
     2221 cgtaccggtc tgatgggcga acgttttggt ggtcagatcc tcgataccgt tgatatcgaa
     2281 aactacattt ctgtaccgaa gactgaaggg cagaagctgg caggcgcact gaaagttcac
     2341 gttgatgaat acgacgttga tgtgatcgac agccagagcg ccagcaaact gatcccagca
     2401 gcagttgaag gtggtctgca tcagattgaa acagcttctg gcgcggtact gaaagcacgc
     2461 agcattatcg tggcgaccgg tgcaaaatgg cgcaacatga acgttccggg cgaagatcag
     2521 tatcgcacca aaggcgtgac ctactgcccg cactgcgacg gcccgctgtt taaaggtaaa
     2581 cgcgtagcgg ttatcggcgg cggtaactcc ggcgtggaag cggcaattga cctggcgggt
     2641 atcgttgagc acgtaacgct gctggaattt gcgccagaaa tgaaagccga ccaggttctg
     2701 caggacaaac tgcgcagcct gaaaaacgtc gacattattc tgaatgcgca aaccacggaa
     2761 gtgaaaggcg acggcagcaa agtcgttggt ctggaatatc gagatcgtgt cagcggcgat
     2821 attcacaaca tcgaactggc cggtattttc gtgcagattg gtctgctgcc gaacaccaac
     2881 tggctcgaag gcgcagtcga acgtaaccgc atgggtgaga ttatcattga tgcgaaatgc
     2941 gaaaccaacg tcaaaggcgt attcgcagcg ggtgactgta cgacggttcc gtacaagcag
     3001 atcatcatcg ccactggcga aggtgccaaa gcctctctga gtgcttttga ctacctgatt
     3061 cgcaccaaaa ctgcataaga agaagtaaga ttcacctgca attgcttagc cgccggggtc
     3121 aaacctagcg gcttttttat ggcattaaaa agccctgccg ggatgttcca ggcagggcgg
     3181 cataaatact tttatctgtt agcgaacgac cagtacaggc agattggcgt ggcggatcac
     3241 gctcgaggcg ttagaaccta acagatgggt cgaaatcgat gggttgcgag aaccgataac
     3301 tacaacatca gcccccagtt cttctgccaa ctcattgact tcatcccgca cgctaccaaa
     3361 acggacatgt tgtttaatgc gggaaggatc gatggtgaag tggctgacca tcgtttgcag
     3421 acgttcttct gcttcatgtt gcagatgctc ttcaaaacga cgcacatcag cggcaaaacg
     3481 gtccaggctc aggctggctg acccgggtag tacgtgaagt agatgaataa ctccgtcatc
     3541 ctgggtgagg aattcagcgt ggcgaacagc tttgtcgctc aattccattt caaatacatc
     3601 aactggcata atgattgtct tatacataac cctttctccc tgttaatcat gaacaaatca
     3661 ttcgccatga ttataatatt tatccctgat atttgtctgg ttctttttcc ttacgaacgg
     3721 tttctgtgat taatatattc tcactgaaca ccaggaattc tcccaaaacc tgcggtaccg
     3781 cccgttttcc cgctgtgata gctaccctta aagactgact cttttttgaa ctgtctctgg
     3841 aggttgcatg aaagcattga cttatcacgg cccacatcac gttcaggtag aaaatgttcc
     3901 cgatccgggc attgaacagg cagatgatat tattctgcgt attacggcaa cggcgatctg
     3961 tggctctgat ctccatcttt atcgaggcaa aataccccag gttaaacatg gcgatatttt
     4021 tggtcatgaa tttatggggg aagtcgttga aaccggaaag gacgtaaaaa atttgcaaaa
     4081 aggcgaccgg gtggtaattc cgtttgtcat tgcttgtggc gactgttttt tctgtcgaat
     4141 gcagcaatat gccgcctgcg aaaataccaa tgcgggtaaa ggcgctgcgc tcaataaaaa
     4201 acagatacca gctcccgcgg cattgtttgg ttatagtcac ctgtatggtg gcgttcctgg
     4261 tggacaggcg gaatatgtcc gcgtccctaa agggaatgtg gggccgttta aagtaccgcc
     4321 tttgctttca gatgataaag cccttttcct ttctgatatt ctgccaacgg catggcaggc
     4381 agcaaaaaat gcgcagatcc aacaaggttc aagcgttgca gtctatggtg ccggtccagt
     4441 gggattgttg acaatcgcct gtgcacggtt gctcggtgcg gaacagattt ttgttgttga
     4501 tcatcatccc taccgcttga gtttcgccgc tgaccgctac ggcgcgatcc cgattaattt
     4561 cgatgaagat agcgatccgg cacagtcaat tattgaacaa acggcaggtc accggggcgt
     4621 ggatgcagta atagacgccg tcggttttga agcgaaaggc agcaccacgg aaacggtgct
     4681 gactaacctg aaactggagg gcagcagcgg taaagcgttg cgccagtgta ttgcggcggt
     4741 caggcgtggc ggcattgtta gcgtaccggg cgtctacgct ggatttattc acggtttcct
     4801 gtttggtgac gcctttgata aagggttgac gtttaaaatg ggacagaccc acgttcacgc
     4861 atggctggga gaattattac cgttaattga gaaaggatta ctgaaaccag aagaaattgt
     4921 tacccactat atgccgtttg aagaggccgc ccggggatat gagattttcg aaaaacgtga
     4981 agaggagtgc cgtaaggtga ttctggtacc cggtgcacaa agcgcagagg aggcgcagaa
     5041 ggcagtttca ggtctggtga atgcgatgcc ggggggaaca atatgatcgt caggagtggt
     5101 tttcgaggta aaggacagcc atgacgataa tcgccgccat aatcagaaat cctatcagga
     5161 tgtaaaatgc ttctgccatg gttattccca caaacgaaac ggaataattt tgcagcaaaa
     5221 gtgaacagtg agaaccagga aaaattgctg attttgcgta aagaggatgc gagtgcatcc
     5281 tctgggcaaa gcgagttatc gcttgtgcag atgggattaa agcaggtagt cgccagcagc
     5341 ttctggctgg tattcgagtt ccagcacttc aaggtgggtt gcaacgccgc ccggaagttc
     5401 ccagtgaatg gaatcgccaa cgcgcagccc cagcagtgcg gcacctaccg gagccataac
     5461 ggaaagctga gtattgctat cggtcatttt tgccggatac accagcgtgc gcacacgcac
     5521 ttcgccatcg ctaagattgc ggaatttaac ccggctgttc attgtcacca cgtcgtgtgg
     5581 catctcttct ggcgaacaca tttgggcgcg atccaactct gcgtttaacg cgtcggcgat
     5641 tggcaaacca gcataggcgg gttgctccag cagaatatcg atgcgttcgg catccaggtc
     5701 gttaatgatg atagttggtc tggacatttt tactccatgt cgtcggtact gcgagtgtcg
     5761 cagataaaca tacccaaaag aaaaccctca ccgtcaggcg gcgagggttt aactcacatg
     5821 atgatactga ctgttgctca ctctttgaag tgatttgcgt cacattcagg gaattcctca
     5881 atgcagcgca ttatgtataa ttcttaatcg tcacggttta tggaagacga attagcgtgt
     5941 tttgtaaatc agatgattaa taacccactt tatcaatcac aaaggttttg ccacagttgc
     6001 ttgggtgtgg ttgtggcaag aatgagtttg cagaaagcgg agcgttgatg gcgtcagctt
     6061 tgatcgagat ctgaatttca gtcaaatacg cagggttacc ctggcacgtt agcttaactg
     6121 ctttcacgtt ctcttttccc ctgcttttgg caaaggcggc gtcaaagtca cggcggctca
     6181 ctgttttacc gtaattatcc gcaaggaatt ttccggcttc gctctcttta atttcttgat
     6241 tcaggcgtac catcgtaccg aagtatgcgt ccggatcaaa accaaagcag gcaccgtgtt
     6301 tggcatattc gtagcgttcc aggcaggaac gtccgccagc tcctggcatg acttcactta
     6361 gtttagcggc cgtttccagt gataacccgg tttccggcga tgaacacatc cggctggcgt
     6421 gcgcttctgg tagattcggg attgggcgag tagcgcaacc gaagcgcatc cagcggcgtt
     6481 catcaacacc acgggcagca accgatttag gcaatcctgg ccacagacca tgtacggtca
     6541 gaaaatcagc tttgttggtc gtttcggttt gcaggcgaca ttcatctcgt tcgttacgat
     6601 ttcgatcgtg ttgactctgg caaaatccgg tttgccagga gagggccagg acatagcgat
     6661 caaaatcgcc atactgtttt gcctgcaacg ctacggcgtt ggcagaagag aagggaagca
     6721 gagaaaccgc gagcaacgcg gcgttacgcc agaattcttt cataatgggt gtggaactca
     6781 tacatacact gaatactatc tattaaatca tcacgcccgc caggtgggct tacctggcgg
     6841 gcgtgatgat ttattcagcg tttggcgaac gtattagttc cacatggcga gaatcggcca
     6901 gccaaccaac agcaacatag agatataaat caccccgaag attgcgccaa gacgccagta
     6961 atcttttgat ttcacatagc cacagccgta aataatcacc ccaggaccgg ttgcataggg
     7021 cgtcagacag cccatgatac cgatagacag caccagcagg atacacagtt gttccattgg
     7081 tacgcccgga atacctttac cgacggccag aataaccggc agcatggttg cggtgtgcgc
     7141 agacaggctg gcaaacaggt agtgtgcaaa gtagaacacc agaaccagta caatcaccgt
     7201 tgcgtttggt gagaatcctt ccaggtgcgt actcatggta ccggcgaacc agtcaataaa
     7261 gccagaacga gtcaggccgt tagccatcac aaccagagtt gccaggttga ccagcgtgtt
     7321 ccatgcgctg ttatagcggg taatatcttt ccaaggcaca acgtgcaggg ccagcattag
     7381 cgaaactgcc agcagaccaa ctgcagtagc attaatgact tcactgccaa aaacccacaa
     7441 acctaagctg agcaatacaa ggccaatcag tgtccactct ctgcgtgtca gcgcacccat
     7501 ggttttcagt tcatcacccg cccaggttgc cacttcttca ctgtgtgtga tttccggttt
     7561 gtacagcacg taggaaagcc acggcgcaat gataagcaag ataaccctaa ccggcaggaa
     7621 gcagaggaac cactgcaacc agctaatctg gataccggca attttgctga cgaactccag
     7681 acccagcacg tttggtgccg caccggtgac aaacatggac gaactcagac tggtactaat
     7741 gaccatcatc cacatcaaat agccgccaat acgacgcgcg gacggatcgt tggggaatga
     7801 tttaaacaac ggcggcaggt ttttaatgac cggaaaaacc gtacccccgg tacgcgcggt
     7861 gttggacggt gtaaacggcg ccagcagaat gtcgataatg acaatcgcat aacccaacgt
     7921 cagcgtgcgt ttgcccatga atttcaccag gaaaagggca atgcgacgac ctaacccgga
     7981 aacttcatac cctaatgcaa aaataaatgc gccaaatacc agccataccg tggtgctgga
     8041 aaaaccagcc agaccccatt tcagcgcctg tttttgcgca ttaaacgctg ggtcagctaa
     8101 ttctttggca tcaaagagca ggtaattact gccaataacg caaatagtaa ccgcaataaa
     8161 actgatcgct gttgccggaa ttggctcgag gatcatgccg acaatcattg ccacaaacac
     8221 agcgaagtaa tgccatgcct gtggtggcat accgttgggg acagggataa gaaacatgac
     8281 acccatcacc accagtgggg ccaatagttt ccatatatta tcttttgcta aagacatacg
     8341 ggttctccga aaattaatat ttccaaattt atcaagtgct taaataatta aatctgtgct
     8401 aaaaaccagg taaggatcag caggtcggca ctgccgcctg gactgagatt tcgttcgata
     8461 cactccctgt cgaactgccg gagataatcg agatcggcgg gggttcgaat gccccctttt
     8521 tgcaataatg tttgcgcctc gcgctgtagc cagcgcaggc cccctcgcca ccgcgcgaag
     8581 caacgttggt atcgccgttg atcgccatca gtaggagcaa ggtatcgagc aatgccagtt
     8641 caggatctaa cccctgatcc agcagagtga ggtaatgcgg caaggcgtgg ttgatcacca
     8701 gtggataacc cgcttcggct tcaccgcgtg cgccggtaag ggcaagctgc tggtacaacc
     8761 gttgacctgc cgtcagttgt gaattattgg tacgcagttc gcgatcggtc aggccacggc
     8821 agaaacttgc cgccgtagaa caaacggttg ttggcgttag cgactggttg agctgaagca
     8881 aacggccaat tgccgcacac agtagcccta aagaaaaaat gctgccctta tgcgtgttta
     8941 cgcccgcggt ggcgcggaac atatcacctt tgcaagccat accaattggg cgtaatccgt
     9001 ggagtaccgc ttctggtgcc atttccgcac tacaggcacc aaattcaatg aaacggggta
     9061 gccagccctg aatcgccagc gcgctgcggt ggaaatcttc cagcgccata tctttatgcg
     9121 caccgcagtt aatgcgatcc acgagacctg gtttcggtga cagattgact tcagtcagca
     9181 tggcgcgcca gcccagcagg gcgtactcat cgattaatga cgtcgcaagc tttgtggttt
     9241 tagttgacgt tgcaggcatc gacatcgttc agcagtgcct ccatgcggtt gagtaaatcg
     9301 gtcagttgat gggttttcca cgcgcgcaga cggctgcgct ttgctcgcac aacaggcagc
     9361 ggcgaggcgg cagtgaatag tcgcggcggg agagaatttc gccttcgggc gtcaggacat
     9421 cgatatccca taaccgcccg agaggatgac tatgttcaag ctcaatggtg gcgagcttga
     9481 ggtcgcgagc aggggcggca atgctcaaca taccctccgg cccgctggcg gaaaccagtg
     9541 cagcctgctc ctgaatttgc cagccctgtt ttgcggctaa ggcacgcaag gctgtcacgc
     9601 catgattaaa aattcggcgt gtgacctcac tgtctttaat cggcctaggc gcaaccacgg
     9661 taaaggagac cagtggaaca ggatggcgct tgagccagac gtgttgccgt gcttgccttt
     9721 catcccggct gacgagcagc tcgggaattg acaccgcatg gtggctggcg agttcaggaa
     9781 gcaggtgcat ggcttattcc ttcacctgat gtacaacatc gatcaccgag ccatcgcggt
     9841 aacgcacaac ggcaacgacg cggtcggtga attcaatcgg ctgtggttca ccggtcagca
     9901 gacgcgcacg ttcacgcagc cactcaatgg aaaccacttt aatgcccgct tcctgcagac
     9961 gttctgccag ctccggacgt gccgggttaa ctgcgatacc gtggtctgtg accagaatat
    10021 cgacactgga gcccggggtg atgcaggtca gcacgttatc caccagagtc ggaatacgac
    10081 cgcgtaccag cggcgcgacg atgatggaaa gcgcagaggc aatcgcggta tcgcagtgac
    10141 caccggaagc accacgcagt acgccgtcag agccggtcag cacgttaacg ttgaactggg
    10201 tgtcaatttc cagcgcgctc agtaccacca cgtcgagacg atcaaccgat gcgcctttcg
    10261 aaccccagtt agcgtactgg ttggcgctga tttcgatgtg attggggtta cgggccagcg
    10321 attgcgcagc atggctgtca aagctctgca catccagcag tttgcggatc agaccttttt
    10381 cgtgcagatc aaccatcgtt gcagtaatac cgccgagggc gaagtcggcg cgaatatcgc
    10441 ggctacgcat tttgtcttcc aggaaacggg ttaccgccag cgaagcgccg ccggtgccgg
    10501 tttgcatgga gaaaccttct ttgaagtagc cagagttgac aatcacatcc gcagcgctac
    10561 gggcaataag cagttcgcgc gggttagtgg tcatacgggt cgcaccagcg ccgatttttg
    10621 cagcatcgcc aacgcgatca actttgacga tcaaatcaac ctgctcttgc tcaatgcttg
    10681 ccggattatg cggataaggc agcagttctt cggtaagcat cacgacctgt tttgcgttgt
    10741 cggcatcaac cattgcatag ccgagggagc cacagcaggc tttaccggtg taaccgttgg
    10801 cattaccgaa ttcatcacag gatgggacgc cgaggaaagc cacgtcgata ttcagt
//
LOCUS       DAZPEB010000138        10764 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZPEB010000138 DAZPEB010000000
VERSION     DAZPEB010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10764
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.141"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(95..1954)
                     /gene="gss"
                     /locus_tag="ACHURJ_003056"
     CDS             complement(95..1954)
                     /gene="gss"
                     /locus_tag="ACHURJ_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFR1281912.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2159..3025
                     /gene="yghU"
                     /locus_tag="ACHURJ_003057"
     CDS             2159..3025
                     /gene="yghU"
                     /locus_tag="ACHURJ_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFR1281913.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPICVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3054..3135)
                     /locus_tag="ACHURJ_003058"
                     /pseudo
     CDS             complement(3054..3135)
                     /locus_tag="ACHURJ_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3148..3396)
                     /gene="hybG"
                     /locus_tag="ACHURJ_003059"
     CDS             complement(3148..3396)
                     /gene="hybG"
                     /locus_tag="ACHURJ_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFR1281914.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3409..3750)
                     /gene="hypA"
                     /locus_tag="ACHURJ_003060"
     CDS             complement(3409..3750)
                     /gene="hypA"
                     /locus_tag="ACHURJ_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFR1281915.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3743..4231)
                     /gene="hybE"
                     /locus_tag="ACHURJ_003061"
     CDS             complement(3743..4231)
                     /gene="hybE"
                     /locus_tag="ACHURJ_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFR1281916.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4224..4718)
                     /locus_tag="ACHURJ_003062"
     CDS             complement(4224..4718)
                     /locus_tag="ACHURJ_003062"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFR1281917.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4718..6421)
                     /gene="hybC"
                     /locus_tag="ACHURJ_003063"
     CDS             complement(4718..6421)
                     /gene="hybC"
                     /locus_tag="ACHURJ_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFR1281918.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6418..7596)
                     /gene="hybB"
                     /locus_tag="ACHURJ_003064"
     CDS             complement(6418..7596)
                     /gene="hybB"
                     /locus_tag="ACHURJ_003064"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFR1281919.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTREELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7586..8572)
                     /gene="hybA"
                     /locus_tag="ACHURJ_003065"
     CDS             complement(7586..8572)
                     /gene="hybA"
                     /locus_tag="ACHURJ_003065"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFR1281920.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8575..9693)
                     /gene="hybO"
                     /locus_tag="ACHURJ_003066"
     CDS             complement(8575..9693)
                     /gene="hybO"
                     /locus_tag="ACHURJ_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFR1281921.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9882..10169)
                     /gene="yghW"
                     /locus_tag="ACHURJ_003067"
     CDS             complement(9882..10169)
                     /gene="yghW"
                     /locus_tag="ACHURJ_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFR1281922.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10288..10764)
                     /gene="yghX"
                     /locus_tag="ACHURJ_003068"
                     /pseudo
     CDS             complement(10288..10764)
                     /gene="yghX"
                     /locus_tag="ACHURJ_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="YghX family hydrolase"
BASE COUNT         2605 a         2969 c         2676 g         2514 t
ORIGIN      
        1 tgaataaata ttagaacata taaatattga atattttgat taatccccgt actgattatt
       61 cttcataatc agtacggggt tgctacaaca tgtattactt tttcaccaca attaacggtt
      121 caatatcact ctcttttttg atgaccagtg attcatcacc gcgcaaacac gtcccaccgt
      181 agttgccgcc cacggtgaag gtacatacct gaatgtattt accgtccact ttcggcaaac
      241 accacagttg ctgatagatg tttttctgct cggcaaattt accgctggtt ttgtccagca
      301 cctcttcatg atggctgacc aggtcgatat tgctaccaca gcgaccggca atcggtttca
      361 ctgcgtaacc tgttttcacc aattcatcat taacagtgaa atcggtatcc agcaggtaac
      421 gatggtgcgg gaacagcgac cagaggatcg gcagaatcgc tttgttgccg gggatcaccg
      481 tccacagcgg ctcaaagact agcacttccg ggcgcagcaa tacgtcgata agacgcactt
      541 cgttttgcgg atgaccggta cggattggca ccgcagcaaa ctcacggtcg ctaacttcac
      601 gaatctgatc aaacgcggtt tcccacgccc aggttttcca cacgcagtta accagtcgcc
      661 cttccccatc aatcagttgc ccggcagcat cccagcccag ttcatccaat ccacgcaaga
      721 tacgcgtttc aaagcccgcc tggtgcagcg cctgctccat aaactgcgcg tgatagtttt
      781 cctcgatatc tttgtcctgc atgatatgga caaacggacg tgcacgactg tgtttccagg
      841 caccagccaa ttcgttaatc agcccttccg ccggattgaa gccgttgcct ttatagccct
      901 gctccgccca acgttcgagg atcaagcccg cttcggtatg acaggaggcg gagtcggcgt
      961 tgtactcgta aaccttcagg ccacgctcat ccatgcagaa atccatacga ccagtgatca
     1021 tatggtgacg gcgacgctgc caggagagac gcaaacgtgg ccagaggatt ttcggaatgt
     1081 cgaacagcgc cagcaggttg tcatctttca gtaccttgtc ggttgcgtga agatacatca
     1141 ggtgcagctc gttggtggct ttaattagct cctgctcggc actctcggta atggtgtagt
     1201 aatgataagg atcctgattg atcacctgac cgttggcctg cacataggcg ttttgcagcg
     1261 gatctttttc atccagccat ttaccgtcaa actggccttt gttttccagg cgcgctccgc
     1321 tgattttcag cagctcgcct gcaatttccg gctgcggtaa gctgtattca gtatcttccg
     1381 tctggatcat ccagcccaga atggtggtgt catcaaaagt gtctttcagg gtatagcagc
     1441 cgttttcgac caccatctcc agctcacgcg tccactgttg cccttgcggc aacggggaat
     1501 gaatcacgtt ctgttccgca atacggactt tgttgccatg caattgggta atgatggcga
     1561 catggccagt gtctttaaat tcaccgcctt tatcccagat aagaagcgca cccgcgaccg
     1621 gcgcacgcgg cgagccgtta ggaaatgcct gcaatggcag gatgttgtca ttaaccactt
     1681 ctcgcaggaa gcgcagcgag aaaatctccc acgccatacc cacgtcagta aagaccacac
     1741 cgtaattcag aaagagaaaa cggcgagcaa attcaacgca ttgccacttg tggcccatat
     1801 attcgtcgtc gatatagcta cggaatacgg cgtcatcttc gtattcctgc ggatcgagag
     1861 aactgtaatc tgaagagtag attgctaccc cacctggggc gtagcccaat aatgtcccga
     1921 acggggcatc ctggctggtc gttcctttgc tcatcacttt acctttaaca atacaaccta
     1981 agcagttggc gtaattgttg tgctctgatt acctgctcag cgacattaac cggacagagg
     2041 tcagactaat catacacctc atcgcagcgg ctggcgcaaa gggttaaaaa attcacattc
     2101 tgtacagcaa gtgacctgcc acactgcttc aacactacca ctcagaaggc aactcactat
     2161 gacagacaat acttatcagc ccgcgaaagt ctggacgtgg gataaatccg ctggcggcgc
     2221 gttcgccaat attaatcgcc cggtttctgg tccgacgcat gaaaaaacgc tgcccgttgg
     2281 caaacaccca ttgcaacttt attcgctggg aacgccgaac ggtcagaaag taacgattat
     2341 gcttgaggag ctgctggcgc tgggcgttac tggtgcagag tacgacgcct ggctgattcg
     2401 tattggcgat ggcgatcaat tctccagcgg ctttgtcgaa gtgaacccaa actcgaagat
     2461 cccggcgctg cgcgatcata cgcataatcc gccgatctgt gtgtttgaat ctggttcgat
     2521 cctgctttat ctggcggaga aatttggcta cttcctgccg caggatttgg caaagcgtac
     2581 tgaaacgatg aactggctgt tctggttaca gggcgcggca ccgttcctcg gcggtggttt
     2641 tggtcacttt taccattacg caccggtaaa gattgagtac gccatcaacc gctttaccat
     2701 ggaagccaaa cgtctgctcg acgtgctgga taagcaactg gcgcagcata agtttgttgc
     2761 gggcgatgag tacaccattg cggatatggc gatttggccg tggtttggca acgtggtgtt
     2821 aggtggtgtg tatgatgccg ctgagtttct tgatgcgggc agttataagc atgtacaacg
     2881 ctgggcgaaa gaagtaggcg aacgtccggc ggtgaaacgt gggcgtattg ttaaccgcac
     2941 caacggaccg ctgaatgagc agttgcatga gcgccatgac gccagtgatt tcgagacgaa
     3001 tacggaagat aagcgtcagg ggtaagggtt ggtgttcgtc gcagcaagcc atccaggccg
     3061 gataaggcgt tcacgccgca tccggcaatc gtgcacaatg cctgatgcga cgctgccgcg
     3121 tcttatcagg cctacgaaag ctaatcatca cgcgctggta atgtcgtaat ccatttggcg
     3181 cagtgcgtct aatgtggctt tggcttcatc ttcgtcgatg atgctcatgg caaatccgac
     3241 gtgtaccagc acccactggc ccagtagatc ggcagggtta ccttcacaaa tcagggcgat
     3301 attcacgtcg cgcttgatac cacatacttc aacctgcgca agctggtgaa tatcttcacc
     3361 gacagccagc acctggcctg gaacgccaat acacattatt aactccggtt attcaacttc
     3421 aatacttttg acgatcagcg aatcgccggt atcgacacgc aaccgctcgc cgtgacagag
     3481 cggacactgc gcatcgtgct gatgaatctc caccacctgg ctgcaatccc agcaccaggc
     3541 ctgggcgggt ttatagacga tatgtaaatc gcacccttga gccaccgttc cgtggcagac
     3601 aatttcaaaa ctaaaacgga cggcgctctc ctcaacgcag gagagcgcgc caatttccag
     3661 ccacacggcg gtgacgcgct taacatcgtg ctgctccgcc tgccgttgga taatttcaac
     3721 ggcgctctgg caaagcgaca actcatgcat tttcgccact cctgcgacca aacagcaggg
     3781 cgcgacgccc tgcgtgtggt acatccggat tcgtgactgg cagcgaaagg atcattcgtg
     3841 cgcagtcatc cgtcaggcgt tggccctctt caatcgacat actgtgcgaa agcggtgaca
     3901 tcagcgaaca ggagaggtat tgcgaaacac cgtccagttc gccaacggta aaggtcatgg
     3961 tgccatacgg cagttgcaga ccaatttttt cactcacttt gcgcagcggc cagagttgat
     4021 ccgggccggg gaagataact gcactcagca tccacggggt gatcacacac cccgtccact
     4081 gaccttcgaa cagcgtaaaa tcagaaacat acaccggcat tgaaggatgc agaaaagaga
     4141 gatcgtgcat cgaacgccgg gcaatttctt caaacgctgc ctgtacttgc gccttcgggg
     4201 aggtctggaa acctgctatc tcttcagtca tgaatcgcct cccgtgggat ggcttccacg
     4261 cctgattcgc gcagcgcagc cagaacctgc tcaagcgcag gttcaatcat tgcttcaacc
     4321 gtcggcgtta agccgatgtg tggctccagc gattccggga tcacgccgac cagggtcagc
     4381 tttttcggaa actcaccggt gaagcgcagg gccgacaata cgtcggccag gccaagctga
     4441 tgcggagaga ttttgttggt aaacaacgcc ggaacttctt catcccgcag gatcatcatc
     4501 gttcccggcg tgttcttttt cgacacaatg gcatccgcaa taatcagatg atcgcgattt
     4561 gccatgtcgc caagcagctc cattcccgcc gtgccgccat cgaggatctc aacataatcc
     4621 ggcagaatgt atcgttgctc taacgcttcg acaatccgca caccgatggc ttcatcggtc
     4681 agcaaaatat tgccgacccc taagactaaa atacgcatta cagaaccttc actgaaacca
     4741 cttcgttgcc gtcggcatcc actacgtgta ccgcacaggc catgcacggg tcgaaggagt
     4801 gaatggtacg caccacttcc agcggtttat tcggatcagc aaccggtgta cccaccagcg
     4861 actgctcgta aggaccgacg tcatcattga agttacgcgg accagagttc caggttgatg
     4921 gaacaactgc ctggtagtta ctgataatac cgtctttaat aaccatccag tgagagagca
     4981 taccgcgcgg cgcttcgagg aagccaacac ctttgaactc acccgttgcc ggaatgttcg
     5041 gtttcacaaa ggtggtgtga tcgcctttgc cgatattggt gatcagtgca ctgtattgat
     5101 tttgcaggat atcctgcaat tcgcagcagt gaacggtacg accaataata cggcccagcg
     5161 tggagtgcag ctgcgccact tccagcgtgt tgccagtcag tttctgataa atcgcaacga
     5221 tttcattcag tttgttctgg gtagattcgc gacctgccgc cagtttcacc agcatattag
     5281 ccagcggacc cacttctacc gttttgccgt agaaagtcgg tgatttcacc caggaatatt
     5341 tcccgtcgtc agaccaacca tcataagccg gaatggtggt accttcccac ggtgcctgcg
     5401 gcgcttcgtc tttataccag gagtgcttcg cgctttcctg aatgcctttg atcaggtatt
     5461 catcggaatg agaagtgatc ggacgatacg aggacagatc cgcattctca atgtagccgc
     5521 ccgggaacag gaagctgccg tttttactgt cggtcgggaa ttccggcacg ctcaggtagt
     5581 tcaccgcacc tttaccgcgc gtcagccatt ccgggtagaa cgcggcaata actgcggtat
     5641 caaccttata aacctgttca acaaagtcgc tcagcttgtc gatgaaagac ttgatgtaca
     5701 tcaggcgctc aaggttcagc acgcccaaac cgtcgaggtt gatcgggttc gcgacaccac
     5761 ctaccgccag gttctgaatg tgcggcgttt taccgcccag cagcgccacg acgcggttag
     5821 cgtcacgctg gcactccagc gcctgcaggt agtgcgctac cgcaatcagg ttcacttccg
     5881 gcggcagttt catcgccggg tgaccccagt agccattggc gaaaataccc aactgaccgc
     5941 tggcaaccag atctttgatc ttgttctgaa ctttagtgaa ctcttccgga ctgttcagat
     6001 gccaggtcga aacgcctttc agcatttcgg aggctttggt tgggtcagct tgcagtgcag
     6061 aagtgatgtc cacccagtcc agcgccgaaa gctgatagaa atgaacgata tggtcatgcg
     6121 tggtgtgcgc agccagaatg atgttacgga tgtattgcgc gttaaccgga acgtcgatat
     6181 tcagcgcact ttctgccgca cgaacggaag acagcgcgtg agtggtagta catacgccac
     6241 agatacgttg cacaatcatc catgcatcgc gcggatcgcg gtttttcacg atctcttcca
     6301 tgccgcgcca catggtaccg gaagcccatg ctttcgaaac gacgccattt tcgatttcgc
     6361 aatcgatgcg taaatgcccc tcaatacggg ttaccggatc aatagtaatt ctctggctca
     6421 tgctttgctc gcctcatgac gattatgatc gttttgtttt aaaggaggaa gtatcggcag
     6481 tagacgaatg agtacgatgt aagcgcaaat ctcaatagcc acaaaaccaa tagaaatcaa
     6541 cagttcttcc ctggtcggga agtaggcgta accgccgccc gggttgaatg ccaccagcga
     6601 ataggtcaga cgccaggttg cacaacctaa cagtgcgctc agtgctgaca ggaacagcat
     6661 gcgggaatca ttacgcagct tcgccacacg cagaacgacc agcgggaaga gcatcagcag
     6721 gacttcaatc cagaacatca cggagtagaa gtcaccggca aacgctaacg acagcttgtc
     6781 gcgatagata agctcgccaa agcgcagcac gataaaaatc gccagcaaca cactgatggt
     6841 gttggtcagc ttgacgaaca gactcttttc atccggaccg ttgccacgca gacccgcctg
     6901 caccagcgaa ccttcaaaga tgacaatcga gaagcccatg atgaacgccg tcagcagcga
     6961 gaacagcggc aacatttcat agctctgcca caacggatgc accttgtagc ccgccgagat
     7021 catcagtgac cccattgaag actggtgcat ggtcggcaac agcgcgccaa gtgcaatgat
     7081 gaagaacatc accttgttca gacgcttgag agaaaccttc cagcccagac gttcaaacag
     7141 tgccggagca aactccagtg ccatcacgcc gatatagatg gtcatacaga ccgccgtctc
     7201 gaacagtacc gagttcacgt tgaagtgacc cggaatgtag aagtacggca ggttccagta
     7261 gcgacccacg tcgatagtga tcgacaagcc acccagtgag taaccaaaca gactcgccaa
     7321 cagcgccgga cgcaccagcg gatggtattg cccacggtta aagacgtata ccgcccacgc
     7381 cagcgcccag ccgccacagg caaagccggt accaatcagc aggtcaaacg cgatccacac
     7441 gccccacggg aagccgccgt tcaggtcaga gacagagccc agaccgaaca ccagacgctt
     7501 cacaatcagg agcatacaga tgacgattaa cggtccaaaa atcatgaccg gtttactgat
     7561 gattttgccg cccagcggtt gtggatcatg actcatgatc gtctcctccg tcgtgatggt
     7621 cgtttttggt gttgcgacga accagcacgg ttaagcccgc cagcacagcc agtggtagca
     7681 tcatgccttt atacagggtg tgttgaatat tttcggaacg cgcaccggta gaaagatcgt
     7741 ccagtttcgg caggtcgaga ttttcataag gcacacccgt cagtaccaga acctgagtac
     7801 cgccgccctc tttctcgccg tacagatgcg gataatattt cggcaccgta tgcaggtaag
     7861 tgtcgccaga tttcagcgtc tgacgcggat agtggtattc gctgccaggc ttcagcgcca
     7921 gacgtttttt cgcctccgcc atcagctctt cacgcgtacc gaaaatcacc gcgcccgccg
     7981 ggcacacttc tacgcagcca ggcagaccgc ctttatcgag acgttccaca cctttctggt
     8041 tgcacagctc gcacttatgc agcgcaccaa acgggttgtt gtagtcgtac ttcggcacgt
     8101 tgtacggaca ggcgaccatg cagtaacggc aaccggtgca cacgtctttg tcgtaatgga
     8161 caatgccggt tttcggatct tttttcagtg cagagaccgg gcacacagag acacagttcg
     8221 gatcgacgca gtgcatacac tgtttcttaa tgtacgcgta gccgttctcc tcctggtctt
     8281 tgttgacccc tgtgccgctg gtccacacct gaatgatgtt attggtatac ggcgacagtt
     8341 tgtcgttgtt cgaccaggtc tgttcccctt gcgggttacg ttcagggaag ttgatatcct
     8401 gacacttggt gacgcaagcc tggcagccta cgcacaaggt cgagtcgtac aacatcccca
     8461 gcgatcccgg aattggcggg cggttttcag cagccgcatg actgacagac ggcagcgcgc
     8521 ccgtcagcaa tgccccgcag gaggctgctt taataaaatt acgtctgttc acggttattc
     8581 tccccgtgag tcagcgttat ctttcttttg ctgacgaccc agttcacgca ccgccatcac
     8641 gctgacaccg gcaaccagcc caaccacacc gccgagcaaa ccaatagcgc ctgcagagac
     8701 gttaccgccc tctttagcgt taacatccgg tttctgtgaa cgcggagtct gattttcgac
     8761 gttggcaagc tgatggatgc ctttatggaa gccgatacct tcttcgttac agccatagca
     8821 tgggtgacca atcgctaccg gccacacgcc gccaacatcg cagaattgca gcgttgagca
     8881 gttgccgtaa gtttctggcc ctttacagcc gaggtggtac aggcaccagc cttcacggtg
     8941 gccttcatca ccgaactctt tggcaaaacg accagcatcg aagtgcgggc gacgttcgca
     9001 gtgttcgtga atcagacggc cataggcgaa ggtcggacga tttttgtcat ccagtttcgg
     9061 cggtttgccg taagtgatga tgtgcgcaac ggtcgcgagg aagttgtgcg ggttcggcgg
     9121 gcagcccgga atattgataa cggttttgcc tggcagaact tcttgcaggc tgactgcgcc
     9181 agttgggtta actccagctg cggcaacacc gccccacgca gagcaggaac cgatagcgat
     9241 aatcgctgct gcgccttctg ccgctttgcg gatgtgatcc acaatcggct caccagcaac
     9301 catgcaataa ataccgttat cttttaatgg gatggaacca tccacaacca acacatactg
     9361 ccctttgtac ttctcaagtg cgttatgttt gttctcttcg acctgatgac cgaaggcggc
     9421 ggaaagcact tcgtgatact ccagagagat agtctccagc acgaggtttt ctaccgttgg
     9481 atgcgttgca cgaagcagag attccgtaca accggtgcac tcctgcgcgc caatccagat
     9541 aactggcgga cgctgcgggt tagtaaccga ttcggccatc tctgcagcgg ctttgctact
     9601 taaccccatg gtggcggcta atgctgcaca aagcttcatg aaatcacgac ggttaatgcc
     9661 gtgagaatgg atgagggtgt tatctccagt catttatagt tattccgttg cgaagacctg
     9721 gcatatattt tgcctcaatc gcaaaatcaa taatgcgatc gatgcgccat ttaccacaca
     9781 tttattatgg ttatcgatat catgatactg cggcggggcg ctaaaacgaa gggaagaagc
     9841 attagtgtaa ttaattaaag caagataata cgtatgtttg atcaattttc gaggcgataa
     9901 ccggccataa aaaagcgcaa cgtagaacag gaattattct cacgaaagaa atccattacc
     9961 atctctttat ccagtccata gcggcgcgtc agaatacccg cttcccaggc gctaagctgg
    10021 cgatctccgg tcttttcgta catccggtgt gagtagccta atacaaaacc gcgtttataa
    10081 tcggcacaga attttgtcac attaaccgca ctgtcggcat gcgttgcttt taatcccgcc
    10141 attaagcctt taccaaaatg gttattcatc gttcacctca ttcgctatat attatcatat
    10201 atagcaatat tttaggcaaa gaacgacttt tgcgcacgct attttttcat gcttgtttaa
    10261 gacgtaatcc cgtaagagat aaacccatca ggagagatat ttatcgaacc atttcagcgt
    10321 cctttgccag gcaagatcgg cggcagattt gtcataacga ggcgtggaat cattatggaa
    10381 tccgtgatta acccccggat agatatacgc ctcatagacc ttattattgg ctttcaacgc
    10441 cgcctcataa gcgggccagc cctcgttgat tcgggtgtcc agttccgcga agtggagtag
    10501 taaaggcgct tcaatcttcg ccacatcggc agtgggtgcc tgacgaccat aaaacggcac
    10561 cgcgcaggcc agttccggat acgcgacagc cgccgcgttc gatacaccac cgccatagca
    10621 aaatccggta atacccactt atgcctgtcg cttgcggata gcgttgcata aactcaattg
    10681 cggcaaagaa atcattcatc agtttggttg gatcaacctg ctgttgcagc tcacgacctt
    10741 tatcatcatt tcccggataa cctc
//
LOCUS       DAZPEB010000139        10727 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZPEB010000139 DAZPEB010000000
VERSION     DAZPEB010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10727
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.142"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..207
                     /locus_tag="ACHURJ_003069"
     CDS             <1..207
                     /locus_tag="ACHURJ_003069"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1281923.1"
                     /translation="HTHRSKRAPVLPLHICERQQSFLCDCRYGKRLGEKSKRWKSYKK
                     KSRDTSHKTTPPEGGVLLLLLIPV"
     gene            complement(190..531)
                     /locus_tag="ACHURJ_003070"
     CDS             complement(190..531)
                     /locus_tag="ACHURJ_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFR1281924.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVEGLKL
                     IESGDWLDRDK"
     gene            complement(534..4313)
                     /gene="tamB"
                     /locus_tag="ACHURJ_003071"
     CDS             complement(534..4313)
                     /gene="tamB"
                     /locus_tag="ACHURJ_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFR1281925.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPDEKPLGETLKDLFSR
                     PVLPEMTDLHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVTQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTLVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDFAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVLVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(4310..6043)
                     /gene="tamA"
                     /locus_tag="ACHURJ_003072"
     CDS             complement(4310..6043)
                     /gene="tamA"
                     /locus_tag="ACHURJ_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFR1281926.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSCGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            6249..6887
                     /gene="msrA"
                     /locus_tag="ACHURJ_003073"
     CDS             6249..6887
                     /gene="msrA"
                     /locus_tag="ACHURJ_003073"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFR1281927.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNEPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            7210..8553
                     /locus_tag="ACHURJ_003074"
     CDS             7210..8553
                     /locus_tag="ACHURJ_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFR1281928.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(8615..8821)
                     /locus_tag="ACHURJ_003075"
     CDS             complement(8615..8821)
                     /locus_tag="ACHURJ_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFR1281929.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            9146..9700
                     /locus_tag="ACHURJ_003076"
     CDS             9146..9700
                     /locus_tag="ACHURJ_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFR1281930.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLQKLLK"
     gene            complement(9790..10701)
                     /gene="ytfI"
                     /locus_tag="ACHURJ_003077"
     CDS             complement(9790..10701)
                     /gene="ytfI"
                     /locus_tag="ACHURJ_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtfI"
                     /protein_id="HFR1281931.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNNFFNNIQHAFKKKDWV
                     SNYDSLLTLWEFFRCATQIDKDSYQVLSSKNETVKAMDKFLISFSLKDNGAEYTMTLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYAWDILQKKQSDIPKRIKGYIHQ
                     AIGDVSLGYALLEDIVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAVDS
                     DITEEEICEKYKSTIEAFKKEQLALPV"
BASE COUNT         2540 a         2882 c         2542 g         2763 t
ORIGIN      
        1 catactcacc gttcaaagcg tgcccccgtt ttacccctgc acatttgtga acgccagcag
       61 agttttttat gcgattgcag atatggaaag cggctcggag agaaaagcaa aagatggaaa
      121 agttacaaaa aaaaatcgcg agatacatcg cataaaacaa cgccacccga aggtggcgtg
      181 cttttattat tacttatccc tgtctaacca gtcgccgctt tcaattagct ttaatccctc
      241 gacgggtcgt tgataaacgt acatccatgc actcccgtac ggcgtctgaa tcaactggcg
      301 cgcgtattca ccgcccctgg tgcgcaaggc atcaagttcg gccagcgtgg cgttgtcaat
      361 acgataaact tcaccgtgta ccgttccgtt ccccggaact gcgcctggat agtggcccag
      421 gctatacaac tggtagttat cgatactgaa atcgcccagt aactgggcat tggtcatcca
      481 gtgactgttg ccttgtttgt ggcgtaaact gccgtagaca aatattcgca ttgctaaaac
      541 tcgaactgat agagcaaatc cagtgcctgg tctacaccag acacggcttc cagatatagc
      601 ttaggcatca ggcgataacg taacgtgagt gttgctatag agtcaaatat acccacgccg
      661 tatttcactt gcaggcctgg caatacatag ccgctgacca ctacctggga ggagtcgcct
      721 actccctggg tgtcgagcgc taaattgctt acgccaaacg tctcgccgat tttacccaca
      781 atctggccac tttgcgcaac ccccagacca atcagcatcg aggtcattgc cgcactgtcg
      841 ctctgatcgc tctccagccc ttgtccacgt agcaaataag acaatgcagc ttgttgcgac
      901 atcgccgggt cagagaagat ctccgctttc ggttcgtccg ccagaccagt gacgcgaact
      961 ccggcgatta cgtcgtcttc tgtagcatcc gggttacgaa tagcttcaat attaagatac
     1021 ggttgatctg gcggaccaga gaacagtagc tcacccttac gcacaatcag atcctgacca
     1081 taggcatgga agcgcccttc agggatgttg atctgcccgt tcaggcccag cccttgtttg
     1141 tcctgaacga cgttaagatc gcccgtcagc cgcgctttca ggccaaaggc gtcaatgcgc
     1201 acattgttgc caacgtggac aatcaggtta ctgttaatcg gaatcgacgc cgttttcggc
     1261 tcttccggtt gcaggttatc gttaagcatc accacatcgc tggagacgcc tactgcgctt
     1321 tccggcagat cgtgcaccac gatgcgcgcc cacgggacat ccacgcgacc atcgagggta
     1381 aacaggtttg gtgtagcctc gaatacaaca tctggcgata catccattcg taccatcggc
     1441 ggcacggtga tccgcacttt actgcctttc gccgttactc gtgcccgcca gttttcaatt
     1501 tggctccagt cggcatcacc gttcaggtag atttcaccct gctgggtccg tactgtaccg
     1561 gcaagcgtcg agcgcatacc gttaaagttg accgcaagct ggctcggctg catatcaaac
     1621 ggcataaagt tgccgtcgat atccacaccc gtaacctgaa gctgaccaaa caactgcggg
     1681 ctttgcacat caccacccag acgcaagttg gcactcacca tccccgctgc tttttcccca
     1741 cgagtaaaga tggggtttat catcgcaagg ttgaagttac ggatattgac gttgccacca
     1801 agattacggc ggccttgcgg atcggtcacc agcacctgtc catcaaactg gccgttattg
     1861 gtcaggcgga tggtccagcc caattcggca cggttgttac gcaattccgc cgtcagattc
     1921 agtgtctgaa acgccaccgg cagcgccgca tcgttgacgg tttgcgttac ctgcacgtta
     1981 cgccccgaaa gggtgatact gccctgcggc agcccctctt tcgtggtgtc ccaggcaaca
     2041 tccgctttac ccgtgaagat accgctggcc tgagtggttt ctggcataaa cggtttcagc
     2101 atggcgaagt cgaagcggtt gagattcacc accgcacgcc cttcggcccc cgcatcgata
     2161 gtttgcggca cgcacagttc cgcattcggg ttaagccagc agtgtggccc gatgctgatt
     2221 ttttgctcct tattgcggta atccagcgca atatcgcggg tcagcgacca cgggccgacc
     2281 agcgtctgga agcgggtatt gctaagagtt cccttccagc gttcttcttt gcgatcaaaa
     2341 cttcctgcca gattaagctg cccggagaca ggctcgccct gaatccgcaa ctgtagctcg
     2401 tgctgctttt cgctgccttt ggcattcagg gtgacgaggt tgatatttac atccggctgc
     2461 gaaatttgct caacgcgtac gtcgagtttc ccggcgatct gatcggtgga tttaatgtcg
     2521 ccttccacgc gaacctgcgt cacggaaagt tcctgccagc gcaggccgcg cgcggtgata
     2581 tctgccagta gttgtggcgc ttccaccgtg ccgcgtactt tcaccagccc tttcgctgta
     2641 ccgccaagcc ccggcagcgc gttatccagc cccggcgcgt tgatggtggc atcaagattg
     2701 agatctttta cccccagctc gcctttcact tcggcactgt ttggccccag ttccagatga
     2761 agcccaggga tcatccactg catataactg ttgcctttca gcgtgccgtc aacgttcact
     2821 ttgttctgtt tgacgttacc ggtcagcttc agttctggca cctccatctg ccaggtgccg
     2881 ccgtacaggc taccgcgggt tttaatcaag ccattgagtt tcgacggcca ctccgggaac
     2941 tctttggcgg tgttaatgcc attaagcgtt agctcaccgc gccaactaat ggcctgctgc
     3001 cagtcgagca acgccttgag ttcagttttc ccttccagcg ccgcgacggt gagtttgtcg
     3061 agattgacct gctgttcatt acctttggca tcaagggtaa tggtggctgg cgggatctcc
     3121 tgtcccttca ctgccgtacg catagagagc gtgtaatcgg tcattttgcc ggtaagtttc
     3181 agtttcagat catccgcctg atactgcttc tcaccagtga acggccagta aagctgtttg
     3241 ctgttcactt ccacgttgag cggcaatccg gcttccgcca gtcgcgtctg ggcgcgtaaa
     3301 tccatatcca ccggaccgga aagattaacg ccaatctcca gctgttcgcg cagcgcgccg
     3361 cccactttta gcttcacttt ttcacctttc aacggctcca cgttcagtgt gctgttgagg
     3421 gtgatatcca ccggccagtt gtctgacagc tgcgccgtgc cgctggcgtt gacgatcccc
     3481 tggttggaat cgatatccag ggcgtccagt ttagtattgc cgtcaatgct gctcactttc
     3541 agcagcatgg tgcgcacggt gatgtccgtg tcgcccgtca cgcgtaactg ctcgccttta
     3601 aactcttcaa tgttcaggtt aagcggcaaa tgcaagtcgg tcatttccgg caataccggg
     3661 cgagaaaaga gatctttcag cgtttcgccg agcggctttt catccggctg cggattttca
     3721 attttcggtt cgaccacttc ttcctgcgcc acttccgcca ctttcggcag agcaatcagc
     3781 aggcctttca gcgacgtcgg tttcagggtc agggttttct cctgccagtt caggccggag
     3841 gtgaagtcca tcaccgacac cgtggtgtca tcaatcttga tgttgacgtt gtccagcgcc
     3901 acccgtgtca gggtgatggg atacggcgtg gagagatcca gcggaccgct atcttcctct
     3961 tcttcaacct gttcagaagg aggcattttt ttactgtcga tgttgacctg aatgtctttc
     4021 agcgccaggt cattaataca aacgctactg ttccacaggc actcaagacc gaccgccaga
     4081 tgcagattac ccgcttttac cgccacgcct ggctgctcat aacgaacgtc agacaaggtg
     4141 agatcgcgcc agccgccggt gaccttgcca atatccagtc ctggcaccca gcgatccgcc
     4201 gctttaaata caagatgcag gccgctggtg gtgcccacca gaaacgccac cgatcccagc
     4261 aacagtaaga taacgataac cacgccgagg ctgatttttt tccataaact cataattctg
     4321 gccccagacc gatgtaaaac tgtaacccgt gttcgtcttt atccgcgacc ggtacggcaa
     4381 aatcgagttt gattggcccg accggcgatt cccagcgcac gccgaccccg gtaccggttt
     4441 taaagtcgct gcggcgaata tcgcttaccg cttcgccact atcgacaaac accgcgcccc
     4501 accattttcc ggtcacgttg tactggtatt ccagcgatcc ggttatcaac ttcgaggccc
     4561 ctttcaggtc accgttggcg tatttcgggg cgatagattt gtatttgtag ccgcgaatac
     4621 tgcgatcgcc cccggcgaaa aaacgcagat ccggcggtac tttgtcgaaa tcaccggttt
     4681 caatccagcc cagcgtgccg cgtgtaacga aacgatggcg atcgtacagt gtgcggatcc
     4741 agacgttctg cgcctggaaa acggagaaat cgacatctga gccccaggcc gtgttggagt
     4801 agtcgataga gtagcgttgc gagtcgcccc aggttggcat caggccacca caagaacgcg
     4861 tgcggctaat catcacccca ggataaaaca gcatcgtggt gttggtaatt tcaccctgag
     4921 taaagtggtc gagactccag cgcaggttaa tggcacgctg ccaaccgctg gagagatccc
     4981 agtagcgaga agccaccagc gtagtggagt cagattcggt atcgttcagg tcagtgcgct
     5041 taaaaccgcc ctgcaccaaa taatattgtt ccagtggatt cttcagcagc ggcattttat
     5101 agctgaagtc gagggtctgt tccggcgcgg aaatactggt actggtggtc aaactgtgac
     5161 cgtatgagtt catccacggc tttttccacg tcgctttcac gcgcggtccc acgtccgtag
     5221 agtaaccgac cccggtttcg atggtgtttt ctgttcgcgg cgaaaccacg cccgtcaatg
     5281 gtaatacttt cgtttcgcgc gctttatcaa attgtggagc caccaccacc gagttaaacc
     5341 agccggtagc agaaagtcgg cggttcagtt ccgccagatc tttcgattca tactcatcgc
     5401 cctctttaaa cggcaccaga ttttgcaggt attcatcgcg gatttgtgat ccttcaaagg
     5461 tcacatgccc aaagcggtaa cgttcgccac tgttataatc aatatcccag aaggctttat
     5521 gcaggccgag cgcaatgccc agctgcgctt tggtaaattc gctatcgaaa taacctttac
     5581 gcaacgcaat actggttaag gactttttga aattttcata atcgccctgg ttcagtaccg
     5641 taccaatagc cgggcgagta tcgagcaatt tcaaatagtc tttatcggtc cgcgcgccgc
     5701 cgcgcaatac cacatcggtg ccgccaatta acaccggcac acctggcgtg actttggcga
     5761 tcaatacctg ccgccctttc tttggcggtg gacggagatc aaattcaatg gtcggctggt
     5821 aataacccag cgctttcaga ccttcacgga tggcatcatc gacgcgtgcg cgaaagcggc
     5881 ggtctggcgt cacttcatca ctttcaatcg tagaaagctg cgcacgaacg ttcttttcca
     5941 gctgtcccga taacccctcg acctgtagac ggacgttcgc ggcgacggca gatccgctta
     6001 agcagagtaa gcttacacag cataactgtc ggatatagcg cacattttct cctgaatatc
     6061 cttttttcct gcccctggaa cgccgttaaa cggcttaaca aaaatccagt aatatggatt
     6121 aaaaagcaga ctaaacccca aatatttctt atgttttact ttagacctat tcacggtggt
     6181 tattgtgtgc aaatacgcct cttgttacaa ccttaacccc aatgaccgat tttcgggaga
     6241 gcgacaccat gagtttattt gataaaaagc atctggtttc ccccgccgat gccctgcctg
     6301 gacgtaacac cccgatgccc gtagccacgc tgcatgcggt caacggtcac tcaatgacca
     6361 atgaacctga cggaatggag attgccattt ttgcgatggg ttgtttctgg ggtgtggagc
     6421 gtctgttctg gcagttaccc ggcgtttaca gcaccgccgc aggctatacc ggcggctata
     6481 cgccaaatcc gacttatcgg gaagtgtgct ccggtgatac gggtcatgcc gaagcggtac
     6541 gcattgttta cgatccttcc gtcatcagct atgagcagtt gctacaggta ttttgggaga
     6601 atcacgatcc cgcccaggga atgcgtcagg gcaatgacca cggcacgcag tatcgttcag
     6661 cgatttatcc gctgacccca gaacaggatg ccgcagctcg cgccagtctg gaacgttttc
     6721 aggcggcgat gcttgccgcc gatgacgatc gtcacatcac cacggaaatc gctaacgcca
     6781 caccgtttta ttatgccgaa gatgaccacc agcaatatct gcataaaaac ccatatggtt
     6841 actgtggaat tggcggaatt ggcgtctgtc tgccgccgga agcatagcgt tacgggtaca
     6901 aatgtagttt gttgataaag tgctctttgt ctatgcctga tgcggcttaa acgacttacc
     6961 aggcctacaa aatcgtccaa attcaatata ttgcaaggac tgcgtaggcc tgatcggcat
     7021 agcgcatcag gcagtattgc atttatcagc agtctgaatt ttaaccctct ggcgacttta
     7081 cagtacctta cgctatacta gccactgaaa attccggttc actttcttcg aatcggcttt
     7141 caatgtgtat ttcacacaaa ttaatcaact ttcccttccg aggatctggc ctgaaaggtc
     7201 ggataagata tgttaaacag tattttagtc atactctgct tgatcgctgt aagtgcgttc
     7261 ttctcgatgt ccgagatctc gcttgccgcc tcacgcaaaa tcaaacttaa actgctggct
     7321 gatgaaggca atataaatgc ccaacgcgtt ctgaatatgc aggaaaatcc cggcatgttc
     7381 tttaccgtgg tccaaatcgg tctgaacgca gtggcgattc tcggcggtat cgtcggtgat
     7441 gcggcatttt ctccagcttt tcacagcctg ttctcccgct atatgtcggc agagctctct
     7501 gagcaactga gctttattct ctctttctcg ttagtgactg gcatgtttat cctgtttgcg
     7561 gatttaaccc cgaaacgcat cggtatgatt gcgccagaag cggtggcttt gcgtatcatc
     7621 aacccgatgc gcttctgcct gtacgtttgc accccgctgg tgtggttctt caacggcctg
     7681 gcgaacatga tcttccgtat tttcaaactg ccaatggtac gtaaagatga catcacttct
     7741 gatgacatct acgcggtagt ggaagccggt gcgctggcgg gcgtgttacg taaacaggaa
     7801 cacgagctga ttgaaaacgt ctttgagctg gaatcccgta ccgttccgtc ttcaatgaca
     7861 ccgcgtgaaa acgtgatttg gtttgatctc cacgaagatg agcaaagcct gaagaataag
     7921 gtggcggaac atccgcactc taagttcctc gtctgtaatg aagatattga ccacatcatc
     7981 ggttatgtcg attctaaaga cctgctgaac cgcgtgctgg ctaaccaaag cctggcactg
     8041 aacagcggcg tacaaattcg caacacgctg attgtgccgg atacgttaac cctttcagag
     8101 gcgttggaaa gttttaaaac cgcaggtgaa gacttcgcgg tgatcatgaa cgagtacgcg
     8161 ctggtggtgg ggatcatcac cctcaacgac gtgatgacca cgctgatggg cgatctggtc
     8221 ggtcaggggc tggaagagca gattgtcgcc cgtgatgaga actcatggct gattgacggc
     8281 ggcaccccaa ttgacgacgt catgcgcgtg ctggatattg acgagttccc gcagtcgggc
     8341 aactacgaaa ccatcggcgg ctttatgatg tttatgctgc gtaagatccc gaaacgcacc
     8401 gattcggtga aattcgccgg ctacaaattt gaagtggtgg atatcgataa ctaccgcatc
     8461 gaccagctgc tggtgacccg gatcgacagc aaggccaccg ccctttcgcc aaaactgcct
     8521 gacgctaaag ataaagaaga aagcgtcgcg taacccccag aaacatcaac ggctcctgaa
     8581 tcaggagccg tcttattact gcatagcact ttggttaagc catctctgtt tgcagacgca
     8641 taacctgacg gttaacttcg gacatcactg acaaatgcag cttatccttc acttttggga
     8701 taagaatctt acccttatca aattcaaaag cgccaacgtc cttgatgtat aaccgtccac
     8761 ggaacaggat ctttacgtac ttcgccacct gaagtgggtt gtaccgttgg aaaattttca
     8821 ttgttatctc ctgctgagta ttacgcctgt gcggtgccac aatcggccca actattatga
     8881 gacgcaaatt ttaatgctta gtgactatag actatccggg caatgtttcc accgcgtata
     8941 acttttttta cctaaaggtt acaattattc agaattatct ttttaccgaa gcgcggtctt
     9001 cagtataagc attcattttt catatgattt gtgcgcttga ccgcaaactg gcatcacact
     9061 tgcgggaaat tcgataaata gcacatatga ttaaaactca gatccaagtg gtcggatcac
     9121 ctgcatatca taagaaggaa acaccatgac cctacgcaag attctggcac tcacctgcct
     9181 gctgttgccg atgatggctt ccgcacatca gttcgaaacc ggtcagcgag tgccgccgat
     9241 tggcattacc gatcggggcg agttggtgct tgataaagat cagtttagct acaaaacctg
     9301 gaacagcgcg cagttagtgg gaaaagtgcg agtactgcaa catattgctg gtcgcacctc
     9361 tgcaaaagag aaaaacgcga cgctgattga agcgattaaa tcagcaaagt taccgcacga
     9421 tcgttaccag accaccacca ttgttaacac cgacgacgca attccgggtt ccggcatgtt
     9481 tgtgcgcagc agtctggaga gtaataaaaa gctttatccc tggtcgcagt ttattgtcga
     9541 tagcaatggc gtcgcgcgcg gtgcctggca gctggatgaa gaaagttccg ctgtagtggt
     9601 gctggataag gatggtcgcg tgcaatgggc caaagacggg gcgctcacac aggaagaggt
     9661 gcagcaagtg atggacctgc tgcaaaagtt gctcaaataa tggcaataac ataaattctg
     9721 caaaaataat tttcccctat cgttatctat gggatagggg aattaggtct ctgagctctt
     9781 acaccgaact catactggta acgctagttg ctctttttta aacgcctcaa tggttgattt
     9841 atatttctcg caaatctcct cttcggtaat atcactatct acggccagat tcgcacttac
     9901 tatcgcatcc acttgttcgg ctaattcgac ttgttggcaa cctgcattaa ccagaaaatt
     9961 ataaataaga gagtaatctt catcctccaa aacaacagag gaaccattac gaaaacgttc
    10021 atcccctcca tagattttaa gcgcaaagcc caaagattct atgactatgg caaaattatc
    10081 atcaattatt ttttcgtaaa tataccatcc atcgcattct ttaattccac ctcctggtat
    10141 ttggagttca aatttacccc gttttaattt gcttactata tcctccaata aggcataacc
    10201 taaagacacg tcaccaatcg cctggtgaat ataaccttta atccttttcg gtatgtcaga
    10261 ctgttttttc tgcagaatat cccaagcata caatcttctg ttttgttcta acggaaattc
    10321 acggtttttg aaatccatga atgagttata ctcatttatt gttatgggga tttcttcgta
    10381 ttcaaatcca ctgcctcgca atgtcattgt atattcggca ccgttatctt taaggctgaa
    10441 actgataaga aacttatcca tggctttaac agtttcattt tttgatgaaa gcacttgata
    10501 actatcttta tcaatctgag ttgcgcaacg aaaaaactcc cacaaagtta acaggctatc
    10561 ataattcgaa acccaatctt ttttcttaaa tgcatgctga atattattaa aaaaattgtt
    10621 tgaatgtgtt tttgtatgcg atgattttac gaataactcc accgcaccaa taaaattatt
    10681 gtgtctgatc ctgggtaaca tatggctacc ttattttatt tggttat
//
LOCUS       DAZPEB010000140        10694 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZPEB010000140 DAZPEB010000000
VERSION     DAZPEB010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10694
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.143"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            43..111
                     /gene="yncP"
                     /locus_tag="ACHURJ_003078"
     CDS             43..111
                     /gene="yncP"
                     /locus_tag="ACHURJ_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFR1281932.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(89..238)
                     /gene="hokB"
                     /locus_tag="ACHURJ_003079"
     CDS             complement(89..238)
                     /gene="hokB"
                     /locus_tag="ACHURJ_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFR1281933.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            637..2250
                     /gene="trg"
                     /locus_tag="ACHURJ_003080"
                     /pseudo
     CDS             637..2250
                     /gene="trg"
                     /locus_tag="ACHURJ_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
     gene            complement(2288..3210)
                     /locus_tag="ACHURJ_003081"
                     /pseudo
     CDS             complement(2288..3210)
                     /locus_tag="ACHURJ_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
     gene            3427..4770
                     /locus_tag="ACHURJ_003082"
     CDS             3427..4770
                     /locus_tag="ACHURJ_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR1281934.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GGPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEYRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHLGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            4995..6650
                     /gene="opgD"
                     /locus_tag="ACHURJ_003083"
                     /gene_synonym="mdoD"
     CDS             4995..6650
                     /gene="opgD"
                     /locus_tag="ACHURJ_003083"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFR1281935.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6790..7014
                     /locus_tag="ACHURJ_003084"
     CDS             6790..7014
                     /locus_tag="ACHURJ_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFR1281936.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGFDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKGV"
     gene            7077..7613
                     /gene="rimL"
                     /locus_tag="ACHURJ_003085"
     CDS             7077..7613
                     /gene="rimL"
                     /locus_tag="ACHURJ_003085"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFR1281937.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7608..8588)
                     /gene="ydcK"
                     /locus_tag="ACHURJ_003086"
                     /pseudo
     CDS             complement(7608..8588)
                     /gene="ydcK"
                     /locus_tag="ACHURJ_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
     gene            8712..9704
                     /gene="tehA"
                     /locus_tag="ACHURJ_003087"
     CDS             8712..9704
                     /gene="tehA"
                     /locus_tag="ACHURJ_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFR1281938.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATMPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9701..10294
                     /gene="tehB"
                     /locus_tag="ACHURJ_003088"
     CDS             9701..10294
                     /gene="tehB"
                     /locus_tag="ACHURJ_003088"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFR1281939.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKLGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            10597..>10694
                     /locus_tag="ACHURJ_003089"
     CDS             10597..>10694
                     /locus_tag="ACHURJ_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFR1281940.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSG"
BASE COUNT         2737 a         2496 c         2712 g         2749 t
ORIGIN      
        1 aaacatgcac taactgaagt gtaaaagggg tgccatgaga aaatgaatct gctagtcaga
       61 tgcgcgggga aaatccccgc gcttgccctt acctggacgt gcaggccatg agcgcagcaa
      121 cctccttatc accgtcccgg aaccgcagtt cgtagagcgt ttgtcgggtc aggagtgtga
      181 atgtcagaat cgtaatgcag ataatgagca gacacaccac cagagggttg tgcttcatag
      241 cctttctcct tgccggatgg cgggtaagag gctaagatct gaattgctag gttcattcgt
      301 tggcctcggt tgatagaaat atcggtcggg gccttcgtct ttctgattcc cggttagcct
      361 gaaaacagaa agtctcaggc acccgcaggc atcctatgag gtttccttag ggacgaaaat
      421 aatcacttca cgaaattgcg tgctgttttc cagaattttt cgtcattcgg attagccagt
      481 ttagccattc gttactctct tcattccaat agcattaatt ttctctgcaa taattgttgt
      541 aaaaatgtga cgcaaagagg tttttggtca taagtaatta ccgtcaagtg ccgatgactt
      601 tctatcagga gtaaacctgg acgagagaca acggtaatga atacaactcc ctcacagcga
      661 ttaggttttt tgcatcacat caggctggtt ccgttatttg cctgcattct tggcggtatc
      721 ttagttctat tcgcattaag ttctgctctg gcaggccgat cgcgatcagc gtgatgttac
      781 tgcggagatt gagattcgga ccgggttagc gaacagttca gattttttgc gttcagcccg
      841 gatcaatatg attcaggccg gggctgcgag tcgtattgcg gaaatggaag caatgaagcg
      901 aaatattgcg caagccgaat cggagattaa acagtcgcag caaggttatc gtgcttatca
      961 gaatcgaccg gtgaaaacac ctgctgatga agccctcgac actgaattaa atcaacgctt
     1021 tcaggcttat atcacgggta tgcaacctat gttgaaatat gccaaaaatg gcatgtttga
     1081 agcgattatc aatcatgaaa gtgagcagat ccgaccgctg gataatgctt ataccgatat
     1141 tttgaacaaa gccgttaaga tacgtagcac cagagccaac caactggcgg aactggccca
     1201 tcagcgcacc cgcctgggtg ggatgttcat gattggcgcg tttgtgcgtg ccctggtcat
     1261 gacgctgata acatttatgg tgctacgtcg gatcgtcatt cgtccactgc aacatgccgc
     1321 acaacggatt gaaaaaatcg ccagtggcga tctgacgatg aatgatgaat cggcgggtcg
     1381 taatgaaatc ggtcgcttaa gtcgtcattt acagcaaatg cagcattcac tggggatgac
     1441 agtagggact gttcgacagg gcgcggaaga gatttatcgt ggcaccagcg aaatttcagc
     1501 tggcaatgcg gacctgtcat ctcgcaccga agaacaagcg gcggctatcg aacaaactgc
     1561 cgccagcatg gagcaactca ctgcgacggt gaaacagaat gcggataacg cgcatcatgc
     1621 cagcaaactg gcgcaagagg cttctattaa agccagcgat ggcgggcaga cggagtaaaa
     1681 acgatgggcg ctatctccac gagttcgaag aaaatttctg agatcaccgc cgtcatcaac
     1741 agtattgctt tccagacgaa tattctggca ctgaatgctg ccgttgaagc cgcgcgagcg
     1801 ggtgagcaag ggcgtggatt tgccgttgtc gccagcgaag tacggacact cgcaagtcgc
     1861 agcgctcagg cggcgaaaga gattgaaggc ttgatcagtg aatcagtcag gttaattgac
     1921 ctggggtcgg atgaggtggc aacggccggg aaaaccatga gcactattgt tgatgccgtc
     1981 gcgagtgtca cacatatcat gcaggaaatc gccgccgcct cggatgaaca aagtagaggc
     2041 ataacgcagg ttagccaggc gatttctgaa atggataagg tgacgcaaca gaatgcttct
     2101 ctggtagaag aggcctcagc ggcggcggtg tcccttgaag aacaggcggc acgattaact
     2161 gaggcggtgg atgtattccg tctgaacaaa cattctgtgt cggcagaacc tcgcggagcg
     2221 ggtgaaccag ttagtttcgc tacggtgtga aaatgttcaa ggagggatcg acagatccct
     2281 tcacctttca gaacggcatt gattttcgaa tagcgttaat catcaactgg caaccagaag
     2341 agaacatcgc atctacgcgg gtcagtattc caatcggttc gcctgcacca tgtcccggaa
     2401 caggctgggc caccagcgtg gcatgacgca ggtcgtcttt taccgcgcca gaagggacaa
     2461 accacacgta gtcgtattca accgtaagtt gacgagatag cgaagcagac agcgtttcga
     2521 tacaacccga aggaatttta catccctggc tctgtactaa tgcatctgaa tgctggcgtg
     2581 gcgcagtgcc ttctggtgat acaacgaccg gccatttcag cactcggctt agcgttacgt
     2641 tctcctgaag tagcgggtga ttagggcgga caaccagctt cagcgattca agaaacagca
     2701 gttcgtaatt aagcccggtc atcagttcag gatctgacat ccgaccaatg ccgatatcga
     2761 tttccccggt tttcaaaccc gccagaatca tagggttact cattgtcgca acttgcaagg
     2821 tcgtctcttt ttgttgctga tgaaactgac ctataaccga aggtaatatc cccagtgccg
     2881 cagtaggtag tgcaccaatc ctgacgacat cattattaag accttcttta cgatgaagcg
     2941 actgtccggc agtgttgatg gcgtcaagaa ctctgactgc atgcgttaaa aattgttcgc
     3001 cgggtaaggt aagttgcgcc ccctgacgac cacgctcaaa caagcgagcg cccgtcagct
     3061 gctacagttc attcaatgtc ttagagagcg caggttgact caaattaagg gtttcagccg
     3121 cgcccccaaa gttccttgtt gtgcgacagc tacgaatgta tgaaggtggc gcaaacggat
     3181 gcgctgacta aacagactat ttttttccat aagcgatgtt aaaaacgaag cggtgtcgct
     3241 gacaagtgaa gttgtttgat tatgataact tgattgcaaa atataattaa caattaaagc
     3301 aattgtgtta cagcaaaatg gataatattg atgttttcgc ggcgagatca cagtttgtaa
     3361 attcttcccg ccagagtgaa tgcggttacc tacactccag attactgacc actggaggca
     3421 gacactatgg cgaacagcat cacggcggat gagattcggg aacagttttc gcaggcaatg
     3481 tcagccatgt accagcaaga agttccgcag tacggcacgc tgctggaact ggtagctgat
     3541 gtgaatctgg ctgtgctgga aaacaatcct caactgcacg aaaaaatggt aaatgcagat
     3601 gagctggcgc gactgaatgt tgaacgtcat ggggcgattc gcgttgggac tgcacaagag
     3661 cttgctactc ttcggcggat gtttgccatt atggggatgt acccggtgag ctattacgat
     3721 ctctcgcagg caggggggcc ggtacattcg acagcatttc ggcccattga tgatgcttct
     3781 ctggcgcgta atcccttccg cgtttttacc tccttactcc gccttgagct tatcgagaac
     3841 gaaattttgc gccagaaagc ggcggagatt ctgcgtcagc gcgatatctt caccccacgt
     3901 tgtcgacaac tgttagagga atatgagcag cagggcggtt ttaacgaaac acaggcacag
     3961 gaatttgtgc aagaggccct ggaaacgttt cgctggcacc agtcagcaac ggtagatgaa
     4021 gaaacctatc gcgccttgca caacgaatat cggttgattg ctgatgtggt ctgttttcct
     4081 ggatgccata tcaaccacct gacgccacgt acgctggata ttgaccgggt gcagtcgatg
     4141 atgcctgaat gcggaattga acccaaaatt ctcatcgagg ggccgccgcg ccgcgaggta
     4201 ccgattttac tacgccagac cagctttaaa gcactggaag agacggtgtt gtttgcgggg
     4261 cagaaacagg gcacgcatac cgcgcgcttt ggtgaaattg agcagcgtgg cgtggcatta
     4321 acgccgaaag ggcgacaact gtatgatgat cttctgcgta acgctggaac cgggcaggat
     4381 aatctcactc accaaatgca tttacaggaa accttccgca cttttcctga cagtgagttt
     4441 ttaatgcgtc agcaagggtt ggcatggttc cggtaccgtc tgacgccttc gggtgaggcg
     4501 catcgtcagg cgattcatct tggagatgat ccacagcctt taattgaacg tggttgggta
     4561 gtggcgcaac ccatcaccta tgaagatttc ttgcccgtta gcgcggcggg gatcttccag
     4621 tcaaatctgg gtaatgaaac gcagacacgc agtcacggta atgccagtcg cgaagcattt
     4681 gagcaggcgt tgggttgtcc ggttttggat gagttccagc tttaccagga agcggaagaa
     4741 cgcagtaaac gtcgctgtgg tttgctttaa attctgacca tccgcctttg caaaaatttg
     4801 cctgatttta caaacgaatc aggctcatcc catcgacata aaaaaaatgc cgatttatgc
     4861 atattctctc agttcaacaa ttggattatt aataaatatt gtctagagtg agcggtcata
     4921 aataagcact ttcttgccgc tgaaaacgac cagcgcggga ccattcacaa caccagaagg
     4981 actcactttc aggtatggat cgtagacgat ttattaaagg ttcaatggct atggccgccg
     5041 tgtgcggtac cagcggcatt gcttctcttt tttctcaggc ggcattcgcg gcagattctg
     5101 atattgccga cgggcaaacc cagcgttttg acttctccat tctacagtca atggcgcacg
     5161 acttagcgca aacagcgtgg cgtggtgcgc ctcgtccgtt acctgacacg ctggcgacaa
     5221 tgacgccgca ggcttataac agtattcaat acgacgccga aaaatcgctc tggcataacg
     5281 ttgagaaccg tcaactggac gctcagttct tccatatggg aatgggattc cgtcgccgcg
     5341 ttcgtatgtt ttctgtagat ccagcaacac atctggcgcg tgaaattcac tttcgcccgg
     5401 agttgttcaa atacaacgat gcgggtgttg atacaaaaca attagaaggg caaagcgatc
     5461 tcggctttgc cggttttcgc gtgtttaaag cccccgaact ggcgcgccgt gatgtagtat
     5521 catttctcgg cgcgagttat ttccgcgccg ttgatgatac atatcaatac ggtttgtcgg
     5581 cccgcggcct ggcgatcgac acttacaccg acagtaaaga agagttcccc gactttaccg
     5641 ccttctggtt tgatacggta aaaccggggg caactacctt taccgtttat gcgttgctcg
     5701 atagcgccag cattactggt gcctataagt tcactatcca ttgtgagaaa agtcaggtga
     5761 ttatggatgt ggaaaatcac ctgtatgcgc gcaaagacat taaacagctg ggcattgcgc
     5821 cgatgaccag tatgttcagc tgcggtacta atgaacgtcg gatgtgcgat acaattcatc
     5881 cgcaaattca tgactctgat cgtctgtcca tgtggcgggg caacggcgag tggatttgcc
     5941 gtccgctgaa taatccgcaa aaattgcagt tcaatgctta caccgacaac aacccgaaag
     6001 ggtttggttt attgcaactg gatcgtgact tctcccatta tcaggacatt atgggctggt
     6061 ataacaaacg cccaagtctg tgggtggaac cgcgtaacaa gtggggtaag ggcaccatcg
     6121 gcctgatgga aatcccaaca acgggcgaaa cgctggataa cattgtctgt ttctggcagc
     6181 cagaaaaagc tgtaaaagca ggtgatgagt ttgcattcca gtatcgtctg tactggagtg
     6241 cgcaaccgcc tgttcattgc ccattagcgc gcgttatggc gacgcgtacc ggcatgggcg
     6301 gtttcccgga aggttgggcg ccaggtgaac actatcccga aaaatgggcg cgtcgttttg
     6361 ccgtcgattt cgttggtggt gatctgaaag ctgccgcgcc aaaaggcatt gagccggtga
     6421 ttacgctttc cagtggggaa gcgaagcaaa tcgaaattct ctatattgaa cccatcgatg
     6481 gttatcgtat tcagtttgac tggtatccga cttcggactc cactgatccg gtcgatatgc
     6541 ggatgtatct acgttgtcag ggggacgcta tcagtgaaac atggctgtat cagtatttcc
     6601 cgccagcgcc ggataaacgt cagtatgttg acgaccgcgt gatgagttaa tcgttttttc
     6661 ttcggcacct tcttcgggag gtgccgtctg gttaaacacg atcccgctcg catttttccc
     6721 taagttaaat gagtaatctg atggtgtgta tttcagatac accttgtcag ccactaacag
     6781 ggagtgcgta tgtttccaga ataccgagat ttaatatccc gtctgaaaaa cgaaaatcct
     6841 cgctttatgt ccttgttcga taaacacaat aaacttgatc atgaaattgc cagaaaggaa
     6901 ggtttcgacg gtcgagggta caatgcggaa gtggtccgca tgaaaaaaca aaagctacag
     6961 ttaaaagatg agatgctcaa aatcctgcag caggagagcg tcaaaggggt gtaaactttc
     7021 ctaagccgcc agccaggcgg cttttttaac aacagcatgg attgactgga gataagatga
     7081 ctgaaacgat aaaagtaagc gaatcacttg aattacatgc tgttgcggaa agtcacgtca
     7141 cacctcttta tcagttaatc tgtaaaaata aaacctggtt acagcagtcg ctaaactggc
     7201 cgcagttcgt tcagagtgaa gaggacacgc gaaaaacggt gcagggtaat gtgatgttgc
     7261 atcaacgcgg ctatgccaaa atgttcatga ttttcaaaga agatgaactt atcggcgtta
     7321 tctcgtttaa tcgtattgaa ccactgaata aaactgctga aatcggctac tggctggacg
     7381 aatctcatca ggggcagggg atcatttctc aggcgctgca ggcattgatt catcattacg
     7441 ctcagtctgg tgaacttaga cgcttcgtga tcaaatgtcg ggtggacaat ccgcaaagca
     7501 accaggtcgc tttgcgcaat ggttttatcc ttgaaggttg cctgaaacag gctgagttcc
     7561 tgaatgatgc ctatgatgat gtgaacttat acgcgcgtat tatcgattca taataaccct
     7621 gccagcggcg ttcgcgtaat gcgctcctcg ccgttaatga cttttggacc acggacatga
     7681 accgtgtcgc catcaaaggc ttcaacgacg gcgtggtcgg ttaactccac atggttttca
     7741 attatcaccg cacctgaaat acggctctca ccttgtatta caacgtgctc gtcgagcaga
     7801 atcggttcac cacgtaccac ggcatttccg ccaatcaaga catgatgttt taacacacaa
     7861 ttaccttcca caatggcata ttccgccacc tgcgaactgt aatgaatcgt cggaatggca
     7921 tcttcttcta tgccagcttt cacctgcgcg tgaccgtaga ctttagcgca atcgcatagc
     7981 cagacgttgt tctcttcatt accttcaaca ctggcaaaat caaaaacttc ggcgcgatgt
     8041 tcaataaaag cataccggac gacggcatcg ccataaattt gtgcctggtg gacaatacgc
     8101 gaggcgctaa cccttgcgcg atcataaatt tggagcagga gttgatggtc gggcgttaag
     8161 ccttgtgcgg cgacgatcat agaatgttga tcaattaagg cgtgaccaaa tattctacat
     8221 tgcccgtaaa ctaatgagtc atgaatggtg acactgtcac ttatatatgc accctgactg
     8281 atttcactat tatcgatcca gacgttatcc gttgcgtaaa cctctcccca cagcacgctg
     8341 gtgccggtga tacgcgtatt accagaaatc accgttccgc cgaacgcaat agcattctgg
     8401 tcgtaaatcc agcaatttcc ttcctatgct aacaccgttt cgcgatcgat ccagccgcca
     8461 gcggttcctg ctatcacatc gttaaaatcg ctaatggcga taatctgccg taataacaca
     8521 ctttttttag tgccatcttc ctgataacta aaggctcgct gttcttcact tagacgatat
     8581 ttgcgcatag cgttttctca caggtgactt actatgaccg tagcaaattc tgcggctctg
     8641 gctatgctcg agaaaattcc ataaaatgca tttcaaatat actttataaa ttaaacaaaa
     8701 tgagtaagaa gatgcagagc gataaagtgc tcaatttgcc ggcaggctac tttggtattg
     8761 tgttggggac gatagggatg ggatttgcct ggcgctatgc cagccaggtt tggcaggtca
     8821 gccactggtt aggggatggg ctggtgattc tggcgatgat catctgggga ttattaacta
     8881 gcgcatttat tacccgactc atacgctttc cgcatagcgt gctggcggaa gttcgccatc
     8941 cagtgctgag cagttttgtg agtttgtttc cggcaacgac gatgctggtg gcgattggtt
     9001 ttgttccgtg gtttcgccca ctggcggtgt gcctgttcag ttttggtgtc gtggttcagt
     9061 tggcttatgc cgcctggcaa actgcgggat tatggcgcgg atctcaccct gaagaagcta
     9121 ccatgcctgg actgtatctg ccgacagttg ccaacaactt tatcagcgca atggcctgtg
     9181 gtgcgttggg ctacaccgac gccggtctgg tgtttttagg cgcaggcgtt ttctcatggc
     9241 taagccttga accggtgatc ttgcagcgtc tgcgcagttc gggagaatta cccacggcac
     9301 tgcggacatc actcggcatt cagctcgctc ctgcgctggt ggcctgtagt gcctggttga
     9361 gcgtcaacgg cggcgagggt gatacgctgg cgaaaatgct ttttggttat ggactgctgc
     9421 aactgctgtt tatgctacgt ctgatgccat ggtatctctc ccagccattt aatgcttcat
     9481 tctggagttt ctcgttcggc gtatctgcac tggcaaccac cggtttgcat ctggggagtg
     9541 gcagcgaaaa tggatttttc catacgctgg cggtaccgct gtttatcttt accaatttta
     9601 ttattgcaat actgctcatc cgtacttttg cgcttctgat gcagggaaaa ttgttagtca
     9661 gaaccgagcg cgctgtttta atgaaagcag aggacaaaga atgatcattc gtgacgaaaa
     9721 ctattttact gataaatatg aattaacccg cacacactct gaagtactgg aagcggtgaa
     9781 agtggttaaa ccgggtaaaa cgctggatct gggctgtggc aatggtcgta acagtcttta
     9841 cctggcagcc aatggttatg atgttgacgc atgggataaa aatgccatga gtatcgccaa
     9901 tgtagagcgc attaaatcca ttgaaaatct ggataattta cacacccgag tcgttgatct
     9961 gaataacctc acatttgata gacagtacga ttttattctt tcgactgtgg tactgatgtt
    10021 ccttgaggct aaaaccatcc cagggctgat tgccaatatg caacgttgca ctaaacttgg
    10081 tggttacaac ctgattgtgg cggcgatgga taccgctgat tatccatgta ccgtcggctt
    10141 cccgtttgcc ttcaaagagg gagaattacg tcgatattac gaaggctggg agagggtgaa
    10201 atacaatgaa gacgtcggcg agctgcaccg caccgacgcc aacggtaatc gtattaaact
    10261 gcgtttcgcc acgatgctgg cacgtaaaaa atgacccgct aagaataagg cgcgtgaaaa
    10321 ttccccacgc tgagatgatt tactgttctt cttttcggta agcatatttt ttatcgaagg
    10381 gatgtgaaat taatcacagt agtcgaagtt ttttagcagc ttaacttact gaaatttaac
    10441 tactgatgat tgacttagcc cctttttcgg cattgactat gtcgtctgaa aaggggctga
    10501 aaaatttatt ttcaccaaca ctttttttgc cacaacacga agcggcgctt tttgctataa
    10561 cttaaaaagt aatataatca tctcaggaaa ctattcatgc gtaccacatc atttgcgaaa
    10621 gttgcagctt tatgcggctt gttggctctg tctggttgtg cctctaaaat cacccagcca
    10681 gataaatatt ctgg
//
LOCUS       DAZPEB010000141        10665 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZPEB010000141 DAZPEB010000000
VERSION     DAZPEB010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10665
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.144"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            311..1609
                     /gene="kgtP"
                     /locus_tag="ACHURJ_003090"
     CDS             311..1609
                     /gene="kgtP"
                     /locus_tag="ACHURJ_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFR1281941.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1606..1929)
                     /locus_tag="ACHURJ_003091"
     CDS             complement(1606..1929)
                     /locus_tag="ACHURJ_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFR1281942.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1975..3330)
                     /gene="pssA"
                     /locus_tag="ACHURJ_003092"
     CDS             complement(1975..3330)
                     /gene="pssA"
                     /locus_tag="ACHURJ_003092"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFR1281943.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYKAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENSGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRLFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3444..6104)
                     /gene="pat"
                     /locus_tag="ACHURJ_003093"
     CDS             complement(3444..6104)
                     /gene="pat"
                     /locus_tag="ACHURJ_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFR1281944.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQRKMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPSALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AISNPLDLRDDASSEHYIKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RIHGLLVQSMANRAGAQELRVVVGHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6136..6834)
                     /gene="tapT"
                     /locus_tag="ACHURJ_003094"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6136..6834)
                     /gene="tapT"
                     /locus_tag="ACHURJ_003094"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFR1281945.1"
                     /translation="MTENTVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREALAEGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6903..7322)
                     /gene="trxC"
                     /locus_tag="ACHURJ_003095"
     CDS             complement(6903..7322)
                     /gene="trxC"
                     /locus_tag="ACHURJ_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFR1281946.1"
                     /translation="MNTVCTHCQSINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7529..8566
                     /locus_tag="ACHURJ_003096"
     CDS             7529..8566
                     /locus_tag="ACHURJ_003096"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFR1281947.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAELVQSITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8614..9303)
                     /gene="ung"
                     /locus_tag="ACHURJ_003097"
     CDS             complement(8614..9303)
                     /gene="ung"
                     /locus_tag="ACHURJ_003097"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFR1281948.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9608..9991
                     /gene="grcA"
                     /locus_tag="ACHURJ_003098"
     CDS             9608..9991
                     /gene="grcA"
                     /locus_tag="ACHURJ_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFR1281949.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10047..10634)
                     /gene="eamB"
                     /locus_tag="ACHURJ_003099"
     CDS             complement(10047..10634)
                     /gene="eamB"
                     /locus_tag="ACHURJ_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFR1281950.1"
                     /translation="MTPTLLSAFWTYTLITAMTPRPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
BASE COUNT         2656 a         2705 c         2740 g         2564 t
ORIGIN      
        1 aaaggccatc cgtcaggatg gcctttttgc attggcgcag aaaaaatgac tgatgcgacg
       61 ctgcgcgtct tatactccca catatgccag attcagcaac ggatgcggtt tccccaactt
      121 gcctacttcc atacgtgtcc tccttaccag aatttatcct taagctcctc aataaccatt
      181 ttcctgctaa ctaaattcat ggttaatgtt gcataataat acgtaacaaa tgcataacat
      241 tttctttacc aaaagaaata aacaaaagtg accaacaaaa gcatcggatt acggcaggag
      301 acataatggc atggctgaaa gtactgtaac ggcagacagc aaactgacaa gtagtgatac
      361 tcgtcgccgc atttgggcga ttgtgggggc ctcttcaggt aatctggtcg agtggttcga
      421 tttctatgtc tactcgttct gttcactcta cttcgcccac attttcttcc cttccgggaa
      481 cacgacgact caactactac aaacagcagg tgtttttgct gcgggattcc tgatgcgccc
      541 aataggcggt tggctatttg gccgcatagc cgataaacat ggtcgcaaaa aatcgatgct
      601 gttatcggtg tgtatgatgt gtttcggatc gctggttatc gcctgcctcc caggttatga
      661 aactataggt acgtgggctc cggcattatt gcttctcgcg cgtttatttc agggattatc
      721 cgttggcgga gaatatggca ccagcgccac ctatatgagt gaagttgccg ttgaagggcg
      781 caaaggtttt tacgcatcat ttcagtatgt gacgttgatc ggcggacaac tgctagccct
      841 actggttgtc gtggttttac aacacaccat ggaagacgct gcactcagag agtggggatg
      901 gcgtattcct ttcgcgttag gagctgtgtt agctgttgtg gcgttgtggt tacgtcgtca
      961 attagatgaa acttcgcaac aagaaacgcg cgctttaaaa gaagctggat ctctgaaagg
     1021 attatggcgc aatcgccgtg cattcatcat ggttctcggt tttaccgctg cgggctccct
     1081 ttgtttctat accttcacca cttatatgca gaagtatctg gtaaatactg cgggaatgca
     1141 tgccaacgtg gcgagtggca ttatgactgc cgcattgttt gtattcatgc ttattcaacc
     1201 actcattggc gcgctgtcgg ataagattgg tcgccgtacc tcaatgttat gtttcggttc
     1261 gctggcagcc atttttaccg ttcctattct ctcagcattg caaaacgttt cctcgcctta
     1321 tgccgctttt ggtctggtga tgtgtgccct gctgatagtg agtttttata catcaatcag
     1381 tggaatactg aaggctgaga tgttcccggc acaggttcgc gcattaggcg ttggtctgtc
     1441 atatgcggtc gctaatgcta tatttggtgg ttcggcggag tacgtagcgt tgtcgctgaa
     1501 atcaatagga atggaaacag ccttcttctg gtatgtgacc ttgatggccg tggtggcgtt
     1561 tctggtttct ttgatgctac atcgcaaagg gaaggggatg cgtctttagt gacgggtcag
     1621 ttgccagacg gtatagccgg tgcttgcacc ggcgacatcc caggccaaat ccttccagct
     1681 ccagccgctc ccttcggggc ggctatccca aagctctttt gacgccccca aactgacaga
     1741 gaacatcaat ccaaacatgg cactgcgatc ccggctcatc ccctgatgct gtgaatattc
     1801 atttccggcg gcggaaagca tcgccgaggc gataaagtgt tgagctttat cctgcccgct
     1861 ccagctatcg ttagccatat ggctgcatcc agaaagtaac aatagcgaac agacaaaaag
     1921 aatacgcaaa acgtggcctc caaaaaacaa accccgtaca ggacggggtt gtgattacag
     1981 gatgcggcta attaatcggt cgatgcggat acggcgcaaa cggcggatga gtttacgaac
     2041 cttcaccgga taatcggcaa tactttgcag atcgcgatag tgcttaacga tggtggtatg
     2101 ctcgcggatc agctccagtt ctttctctcg ctgcggcgca agctccagtt gtggatcgtg
     2161 gatcaaaatg gcgttttcca gatccagacg ccaggcgcgc gggttcaggt tattaccggt
     2221 gatcaacatc cacttatcat caacccacat ccctttcagg tgataggtgt tgtcgtcatc
     2281 tttccataac cgaaccacta gctggtcagt attgacgtaa tactgcaaac ggctcaggaa
     2341 aagacgcaga ttgatctcat agagataagg caatgcgcca attatcttga aaggttcatc
     2401 ttccggaatg tagaagtcgt tcgcggtttt atcaccaaca ataatttcga cctttttccc
     2461 ttcgcgcagc aactggataa tattgcgcac aaggattgct ggcaggttga agtatggcgt
     2521 acagatggtt agtttctgct cggcacaagg cataagatgg aaaatggtct tgttcaacag
     2581 actcgatttc cccagcccca ctagcggcgt tacggaaagc tgatcgttgt cggcatcgcc
     2641 ctggaaatga taagcggcat cacgcagctc ctggcggaac agacgaatat cgttcttgat
     2701 ttccgggctt tttggccgat taacatcatc cagacgatta acgccgcggc cattcataat
     2761 attctgtgta acccattcaa acataatgtc tgacatctta cggttacgga taagatgata
     2821 acggtcgtag cgatatttat cgtgctgatg caggtaaaca tcgttcaggc tggcaccgct
     2881 ataaagtacg ctatcgtcga tgataaagcc tttaaagtgc agaacaccaa gggcttcacg
     2941 ggtattgatt ggaacgccat aaaccggaac atctacgccc gaattttcct gcgccatgcg
     3001 gcagtaccag tcagcgttag tgttagatgc cgcagcgcca atgcgtccac gttgtgcacg
     3061 atgccagtcg accagcaccc gcacatccag ttccggacgc tgccttttag ctttatacaa
     3121 cgcgttcaga atgcctttgc caccgtcatc ctgttcgaga tacagggcga caatgcaaat
     3181 gcgctgcttc gcgctggcta ttttttccag cagcgtctcc cggaagtcgg cgggagcgta
     3241 aaagaaatcg acatcatcaa ctgattgaga aatcttgggt agttgggcaa ggtgttgttg
     3301 atgtttatta cgcttaaatt ttgacaacat cacagtgcat ttcttctctg ttcattgaag
     3361 gatcctctgt gcaatgcaga cgacataagc gggcaataat aacaccagtc ccgattaagt
     3421 agtcaacatt tccagtacct tactcatgat tcctcgcgtt gggcaagatt tagcgtaagc
     3481 ccaacgatcc cctcttcgag ctggatatca acgttaaacc cgagcttgcg ggctagcgcc
     3541 accatgccac gattgtttgg catcgtaata ccattcagac gttgtagtcc gtgatctcgc
     3601 gtataggtaa tcaacttttc cattaagcgt cgacctaagc ctaacccttt gagatccgag
     3661 cgaaccagca cagcaaattc ggcatcgatg ttatcaggat cggaaatcgc acgcgtgacg
     3721 ccgaggatct cttccgtttg atcaatacgt cgtaccgcta caaacgccat ttcccgatcg
     3781 tagtcgatct gtgtcatgtt ggctaaatct tcatgggtaa attcgttgat ctcgctaaag
     3841 tagcggtaat aaagatcttc tttggtgact cgcgaaatga attgctgaag ttgtggctca
     3901 tcttctggca aaatcgggcg gaacaagcag cgttcaccgt ttttcaattc tacccattct
     3961 tccagctgat gcggataagg gcgcactgcc agccgactct cgttatcgcc ttcaaacggc
     4021 gagatatcca gcgtgacatc cagcgcggta aattcactgc cagaagccag caaaggatga
     4081 atatccagac gctgaatttc cgggcaatcg acaatcaagt tggaaacctg caccagaagc
     4141 tggctcaagc ctgcaacatc caatgggcgt agcgcactgc gcgcacgaat ctttttactt
     4201 ttgatcccct gaataaccag atagcgggcc aggttcatgt tcagcggcgg cagtgcgacg
     4261 acggcttgat cttcaggacg ccactccaca ccgccttcac ccagcatgat caacggcccg
     4321 aaaaccggat cgtgcccaac cacaacccgc aactcctgag cgccagcacg gttagccata
     4381 ctttgcacca acaggccgtg gatccgcgcc tgcggccagg ccatttttac gcgatcgaaa
     4441 atggcgtttg ccgcttgctg gacttcattg gctgtacgca agtacagcat gacgccctga
     4501 acttccgatt tatgtggaat atccggcgaa cgcaatttca gcgccaccgg ataaccaatc
     4561 tgttcggcaa tatgcaccgc ttcggtgcta tcgctggcaa tccaggtagg gagcgtgttc
     4621 atgccatacg cttgcaggat gggctgaact tcatgggtat cgagcgacgt agccccttcg
     4681 gcaatcgcct gttgcaacag aagatgcgct tctgcggtat tggaagtcag attgctgggc
     4741 aacgccggcg tttcgcgtag ttgcttctga ttacgccggt actccaccat atgcataaaa
     4801 gcagtgatgg ttccttccgg ggtacggtag gtcggcagcc cggcttcgct gaataaacgt
     4861 cgtgcctctt gcgaggagtg ctcgccgcac cagttcgtca gcaaagagac atatttgctg
     4921 cggggatgat gctttaccgc ttcaattaat acttgcgcgc tttctgttgc gggagcagcg
     4981 gcgctgggcg aatgaataac catcagcgcg tcaaaatcct ggctgtggag cagaatatcc
     5041 agcgttttaa tatagtgctc actgctggcg tcatcgcgta gatcgagcgg gttagatatt
     5101 gccacatgtt ctggcagtgc atcgcgcagt ttctggcagg tttcttcgct tagcgttgcc
     5161 agcttgccat tgcgtgacca taaggcatcc agcgccagcg cggaaggcgc agcaccgttg
     5221 ctgataatca tcagccggtc gccacgtagc gggcgcatat ggctaagggt ttccaccgcc
     5281 gaaaacagct cgtgggtgtc ctgtacccgc aacaaaccgg cacgctgaat agccgcatcc
     5341 catgccgggt ccattcctgc cgtggtgttg agcagtcgct gtgccgccgg gctacgtccg
     5401 cttttaatca ccagaatcgg tttattacgc gaggcactac gggccgccga aacaaagcgt
     5461 cgcgcgtcgc ttaactgttc gagatagagc aggatggcgc tggttttact gtcgcgtgcc
     5521 agatagtcaa gcaattcatc aacgtcgata tccaggctgt cgccgagcgc aataaagtag
     5581 gaaaagccca tcttacgctg ttgcgcccag tcgaggatgg tgttggagac ggcagccgat
     5641 tgcgaaataa acgccagctt gccgcgttta atcggcacag gcgaaaagct ggcattcaga
     5701 ccttgccagg gagccagtaa acccagactg tttggtccaa gcaggcgcat gttatggcgc
     5761 agggcgcagg cgcggagatc ttcgtgttgc gatgccgggg cggaaagaat aatgcaggtt
     5821 ttacagcctt tctcgccgag ctcttccaga agagcaagat tacggctggc attggtacat
     5881 aaaaccgcaa ggtcgggtgt aaagggcaag ctggcaatat ccggccaggc caacacaccc
     5941 aacaccgctt tccaggctgg cgtcaccggg agtaccggtc cgttaaagcc tcccgccagc
     6001 aggttacgca tcatcaggta acctgcgcga ttgggtttca tcgacgcgcc aattaccgct
     6061 atcgattttg gtcgcagtag tgcttccagt cctcgctgac tcataccggt ctcccgcaga
     6121 agtgaccgaa tgattttaaa cgctttctaa ctgttctgct gtgatgctac ccagatgttg
     6181 cgttttccct gccagatagc gtgttttaaa gcgggtaaaa tgctcgccta accctgctgc
     6241 cgccccggta tcgccggcca tatctaacag tgcgatggct acctcggcag tacaatattg
     6301 gccttcagcc agggcttcac gcaggcgata ggcagaaagc cgggaaagat cgacggaaat
     6361 gacgggaaga ttatccagat acggactttt acgaaacatc ttgcgagctt ccggccaggt
     6421 accatcgagc atgataaaca gcggtggctt accggcaggt ggtgtgaaga tcacttcccg
     6481 ttgctcatca gcatacgagg cgggaaagac caccattggc tgataatccg ggttttgtac
     6541 cagatccagc aaatcctgcg agggttcggt acgcgaccat tgaaacgcaa cggtatcagg
     6601 caaaatatca gcaatcagac gcccggtatt actgggcttc attggctcgg tgtcgaacat
     6661 cagcaaacag aagcgacttt ttgcttgtgc tggggtaatt gtcgaacaga gacataattt
     6721 ctctggcaaa agacagcgtt ggcagcgacg aacgcgatta ccgcgggcaa gaaaaggacg
     6781 tgttgcgcgc gcaatacgct cggcgcgtaa ctggagaaca gtgttttcgg tcataagaga
     6841 gcgtcgaaaa aacgccattg tcgcagagga gaaaacgggg cacaagatgc gccccggtaa
     6901 gattaaagag attcgttcag ccagctatcg aacggagctt tcggtactgc gccattaagc
     6961 atgtcgacaa cctgaccgtt tttgaaaatc atgatcgtcg gtatactacg aattccaaag
     7021 cgactgctca attcacgttc agcttcggta ttcactttca caaagcgcac tttaccgcta
     7081 cgctcttgcg cgacatcttc aaaaattggt gcgaaattac ggcaggggcc gcaccacggt
     7141 gcccagaagt cgatcaccac aggtagatca tccttcagca atttgtcgag tgtttcaccg
     7201 gtcgcattaa tcacctctcc gtcaaacaag tcgtgaccgc agcgtccgca ttttgccgca
     7261 tcttcgatcc gatcgtcggg aatgcgattg atggactgac aatgggtaca aacggtattc
     7321 ataactaacc tcgggatgag taaggagact tatcggcaac gacgccggta tgtttctaat
     7381 atgttacata ttatcgggga cattgttcca gacaacaatc tgttttattc aataggtatg
     7441 atagtcgcag gctttggtac gaaatcatgg agaaccgcgc ggacttcggg taatctgcgc
     7501 gcttcgcgca gcgctggtgg agaaaagcat gaacgatgaa atgaaaggta aaagcggcaa
     7561 ggtcaaagtg atgtatgtcc gcagtgatga tgattctgat aaacgtaccc acaacccgcg
     7621 taccgggaaa gggggcgggc gtccaggaaa atctcgtgct gacggtggcc gtcgccccgc
     7681 ccgcgatgac aaacagagtc agccccgtga ccgcaagtgg gaagattcgc cgtggcgcac
     7741 ggtttcccgc gcgccgggtg atgagacgcc ggaaaaggcc gatcacggtg gcatcagtgg
     7801 taaaagtttt attgatccgg aagtgttgcg tcgtcagcgt gcggaagaaa cccgcgtcta
     7861 cggcgaaaac gcctgtcagg cactgttcca gagccgtccg gaagcaattg ttcgcgcctg
     7921 gtttatccag agcgtaacgc cacgttttaa agaagccttg cgctggatgg cggcaaaccg
     7981 caaagcgtac catgtggtgg atgaagctga attgacaaaa gcgtcaggca cggaacatca
     8041 cggcggcgtt tgcttcctga tcaaaaagcg taacggtaca accgtgcagc agtgggtaag
     8101 ccaggcaggc gcgcaggatt gcgttctggc actggaaaac gaatctaacc cgcataacct
     8161 gggtggcatg atgcgcagct gcgcgcactt cggcgtgaaa ggtgtcgtgg tgcaggatgc
     8221 ggcactgctg gagtcggggg cggctatccg taccgcagaa ggcggcgcag agctcgttca
     8281 gtcgattact ggcgacaaca ttgttaacgt gctggatgat ttccgtcagg caggttacac
     8341 cgtagtgacc acttccagcg agcagggtaa accgctgttc aaaactagtc tgccagcgaa
     8401 aatggtactg gtgctgggtc aggaatatga agggttaccg gatgccgcac gcgatccgaa
     8461 cgatctgcgc gtgaagattg acggtacggg caacgttgcc gggctgaata tctctgtcgc
     8521 aaccggcgtt ttgcttggtg aatggtggcg tcagaataaa gcctgataaa tcagccgggt
     8581 ggcaactctg ccatccggca tttccccgca aatttactca ctctctgccg gtaatactgg
     8641 catccagtca atcggcgtct cgccacgttg ttccagccac tgatttgcca gcacaaaatg
     8701 gttgcagcca aagaatccac gatgcgccga aagcggcgac ggatgcggtg ctttcagtac
     8761 atgatggcgt tgcttatcta taatcgcccc tttcttttgc gcatgtgatc cccacaacaa
     8821 aaacaccacg ccttcgcggt gctggttaat caggctgatg actttatcag taaacgtctc
     8881 ccaaccgagg ctggcgtggg aatgcgcctg acctgcgcga accgtcaaca cagtattgag
     8941 cagcagaacg ccctgacgcg cccagctttc gagatagcca tgattagggc gggtgaagcc
     9001 cggaatagta ttttccagct ctttatacat attcaataat gacggaggaa tggcaatgcc
     9061 gggacgaacg gaaaatgcca gaccatgcgc ctgtcccggt ccgtgataag gatcctggcc
     9121 gagaatcacc actttaacgt cacccaactc ggtaaagcgg aacgcgttaa agacatcttt
     9181 ttgtggtgga tagatagtga cgccggactg ccgctcgctg gcgacggtct gaagggtatt
     9241 aagaaaataa ggttgttgct tctcttcagc cagcacgtca tgccaggtta attcgttagc
     9301 catctcgctc tcctgcgaat cttcaatccg cctagcttaa ctgcttcttt ctacagaaca
     9361 aaatcatgct cattttttag acttaaacga gtgcaaaaaa atgtaaattg gtaaaatttc
     9421 acacccgcat aagttgatgt aaaacaataa aatccattta cgaccacaat cgatgtgtgg
     9481 tttttattga tttaaatcaa agaatcaagg gtggttgagg agtatatata cactcaagca
     9541 acaatggttt taccaattag ccgcgacagg ctgaacaaat caaataattt tgccggggag
     9601 gcatcacatg attacaggta tccagattac taaagccgct aacgacgatc tgctgaactc
     9661 tttctggctg ctggacagcg aaaaaggcga agcacgttgc atcgttgcaa aagcaggtta
     9721 tgcagaagat gaagtggttg cagtaagcaa actgggtgac attgaatacc gtgaagttcc
     9781 agtagaagtg aaaccagaag ttcgcgttga aggtggtcaa cacctgaacg ttaacgttct
     9841 gcgtcgtgaa actctggaag atgcagttaa gcatccggaa aaatatccgc agctgaccat
     9901 ccgtgtatcc ggttatgcag ttcgctttaa ctctctgact ccggaacagc agcgcgacgt
     9961 tatcgctcgt acctttactg aaagcctgta aggcttaagt agcaagttac taagcggaag
    10021 agggcgacct cttccgcttt ttttcgttaa tagaaaatgc gtaccgcgca atagaccagc
    10081 aacagggcaa gcacgatatt taactggcga ccatactggc gaaacaatcg ctgaaacaga
    10141 tgccccgcca gcgcccagca cacattgcca aacgtcccaa tcatcgccag caaaacgctg
    10201 acgccaacta cccagcttag cgcctgtgtt tgcggcagaa caaacgtcga cagtgccgta
    10261 acaccgtaca aaatgatttt gacgttcaca aactgcaaag caaagctggc ccaaaagctg
    10321 attggttttg cctgaagtcc gtcttccttt gttgggctgg tggcgatttt ccacgccagc
    10381 cagacaatat atgccgcccc cgcccaactc aaaaggtgta ccgctgccgg gtcaatcact
    10441 gccagtgaaa atgaaatgcc cgcacacagt aacatcacaa tcaaaaatcc cagactcatc
    10501 cctgccagca cgcgggtact ttgacgaaat ccatgcgacg tagcagagct aagggcgaga
    10561 atattgttcg gtcttggcgt catagcggta atcagggtgt aagtccaaaa agcacttaaa
    10621 agggtcggtg tcacttgtta tctcctctgt tttggtagac agtac
//
LOCUS       DAZPEB010000142        10525 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZPEB010000142 DAZPEB010000000
VERSION     DAZPEB010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10525
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.145"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            141..2009
                     /gene="kup"
                     /locus_tag="ACHURJ_003100"
     CDS             141..2009
                     /gene="kup"
                     /locus_tag="ACHURJ_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFR1281951.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSLTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2176..2595
                     /gene="rbsD"
                     /locus_tag="ACHURJ_003101"
     CDS             2176..2595
                     /gene="rbsD"
                     /locus_tag="ACHURJ_003101"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFR1281952.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2603..4072
                     /gene="rbsA"
                     /locus_tag="ACHURJ_003102"
                     /pseudo
     CDS             2603..4072
                     /gene="rbsA"
                     /locus_tag="ACHURJ_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
     gene            4077..5042
                     /gene="rbsC"
                     /locus_tag="ACHURJ_003103"
     CDS             4077..5042
                     /gene="rbsC"
                     /locus_tag="ACHURJ_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFR1281953.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGTIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            5067..5953
                     /gene="rbsB"
                     /locus_tag="ACHURJ_003104"
                     /pseudo
     CDS             5067..5953
                     /gene="rbsB"
                     /locus_tag="ACHURJ_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            6079..7008
                     /gene="rbsK"
                     /locus_tag="ACHURJ_003105"
     CDS             6079..7008
                     /gene="rbsK"
                     /locus_tag="ACHURJ_003105"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFR1281954.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            7012..8004
                     /gene="rbsR"
                     /locus_tag="ACHURJ_003106"
     CDS             7012..8004
                     /gene="rbsR"
                     /locus_tag="ACHURJ_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFR1281955.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(7970..9397)
                     /gene="mdtD"
                     /locus_tag="ACHURJ_003107"
     CDS             complement(7970..9397)
                     /gene="mdtD"
                     /locus_tag="ACHURJ_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFR1281956.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMFIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     RATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGSVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(9420..10112)
                     /locus_tag="ACHURJ_003108"
     CDS             complement(9420..10112)
                     /locus_tag="ACHURJ_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFR1281957.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKKNFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KTCQALLRSPDK"
BASE COUNT         2581 a         2535 c         2776 g         2633 t
ORIGIN      
        1 aaggggggta tttttctttg agccaggtta atgtggccgc atttaggagt acgattttgc
       61 cgttaatcgt gcatactgtg cgcttttttg tgggccaagg gactaagcac acatttcata
      121 tttcaacgaa agactagtct atgagcactg ataataagca atcattgccc gcgattaccc
      181 tcgcggcgat tggagttgtc tacggcgata ttggtaccag cccgttatat acacttcgtg
      241 aatgtttgtc cggccagttt ggttttggcg ttgaacgcga tgccgtgttt ggctttttat
      301 cgctgatctt ctggctgcta atctttgtgg tttccattaa atatctcacc ttcgtgatgc
      361 gggcagataa cgccggtgag ggggggatcc tgacgttgat gtcgcttgcc gggcgtaata
      421 cgtcggcgcg aaccacatca atgctggtga ttatggggct aatcggcggc agctttttct
      481 atggtgaagt cgtcataaca cccgctattt cggtgatgtc agccattgaa ggtctggaaa
      541 tcgtcgcccc gcagctggat acctggatag ttcccctctc aattatcgtt ctcactttat
      601 tatttatgat tcaaaaacat ggcacggcta tggtcggtaa gctgtttgcg ccgatcatgc
      661 tgacctggtt tttgattctg gcaggactgg ggttacgtag cattattgct aacccggaag
      721 tgctgcatgc gctgaatcca atgtgggcgg tgcatttctt ccttgaatac aaaacggttt
      781 cttttattgc attaggggca gtggtgttgt cgattacggg ggtcgaggcg ctgtatgctg
      841 atatggggca ctttggtaag ttccctattc gcctggcgtg gttcaccgtc gtattacctt
      901 ccttgaccct taattacttc ggtcagggag cgctgttgtt aaagaacccg gaagcgatta
      961 agaacccgtt cttcctgttg gcaccggact gggcgctgat cccgctgctg atcatcgccg
     1021 cactggcgac ggtaattgcc tcgcaggcgg ttatctctgg cgtcttctca ttgacgcgtc
     1081 aggcggtacg tctgggatat ttgtcgccga tgcgcattat tcacacctcc gaaatggagt
     1141 cagggcaaat ctatattccg tttgtgaact ggatgctcta tgtcgcggtc gtgattgtga
     1201 ttgtcagctt tgagcactcc agcaacctgg cggcggcgta cgggattgcg gtgaccggaa
     1261 ccatggtgct gacgtctatt ctctcgacta ccgtggcacg tcagaactgg cactggaata
     1321 agtattttgt tgcgctgatc ctgattgctt tcctttgtgt cgatattcca ttgttcaccg
     1381 ctaacctcga taaactgctc tccggcggtt ggttgccatt gagcctcggt acggtgatgt
     1441 ttatcgtgat gaccacctgg aagagcgagc gtttccgctt gctgcggcgg atgcatgaac
     1501 atggtaactc tctggaagcg atgattgctt cgctggagaa atcaccgcct gttcgcgtgc
     1561 ccgggaccgc ggtgtatatg tcgcgtgcaa tcaacgtcat tccctttgcg ctgatgcata
     1621 accttaaaca taacaaggta ttgcatgagc gggtgattct gttaactctg cgtaccgaag
     1681 acgcgccata tgtccataat gtccgtcggg tacagattga acaactgtcg ctcactttct
     1741 ggcgcgtggt ggcaagttat ggttggcgag aaacgccaaa cgtggaagaa gttttccacc
     1801 gctgcggtct ggaaggatta agttgccgga tgatggaaac ctccttcttt atgtcgcatg
     1861 agtcgttgat cctcggcaaa cgcccgtggt atttgcgtct gcgcggcaag ctgtacttgc
     1921 tgctgcaacg taatgcgctg cgtgcaccag atcaatttga aatcccgcca aacagggtta
     1981 tcgaactggg tactcaggtc gaaatctaac gccagacgcc tcctttctcc ataagggggc
     2041 gtttttgttt tcatggttaa tcaccatgta aaacgtttcg aggttgatca catttccgta
     2101 acgtcacgat ggttttccca actcagtcag gattaaactg cgggtcagcg aaacgtttcg
     2161 ctgatggaga aaaaaatgaa aaaaggcacc gttcttaatt ctgatatttc atcggtgatc
     2221 tcccgtctgg gacataccga tacgctggtg gtgtgtgatg ctggtttacc catccccaaa
     2281 agtacaacgc gtatcgatat ggcattaacc cagggtgtac cttcttttat gcaggtgctg
     2341 ggcgtcgtta caaatgaaat gcaggtcgag gctgccatta tcgcggaaga gatcaaacaa
     2401 cataatccgc aactccacga aacgttgctc actcaccttg agcagctgca aaaacaccag
     2461 ggaaatacca ttgaaattcg ttacaccacg catgaacaat tcaaacaaca aaccgcagaa
     2521 agtcaggcgg taattcgcag cggagaatgt tctccgtatg cgaatatcat tctctgtgct
     2581 ggcgtgacgt tctgaggccg tcatggaagc attacttcag cttaaaggca tcgataaagc
     2641 cttcccgggc gtaaaagccc tctcgggcgc tgcgttaaat gtctatccgg gccgcgtgat
     2701 ggcgctggtg ggcgaaaacg gcgcgggtaa atccaccatg atgaaagtgc ttactggtat
     2761 ctatgctcgc gatgccggca cgcttttatg gctggggaaa gaaacgacat ttaccgggcc
     2821 gaaatcttcc caggaagccg ggattgggat tatccatcag gaactgaacc tgatcccgca
     2881 gttgaccatt gccgaaaaca ttttcctcgg tcgtgagttt gttaatcgct ttggcaaaat
     2941 tgactggaaa accatgtatg ccgaagcgga taaattgctg gctaaactta acctgcgctt
     3001 taaaagcgac aagctggtgg gcgatctttc catcggtgac cagcaaatgg ttgaaatcgc
     3061 caaagtgctg agctttgagt cgaaagtcat cattatggat gaaccgaccg atgcgctgac
     3121 cgataccgaa accgaatccc tgttccgcgt catccgcgaa ctaaaatcgc aaggccgcgg
     3181 tattgtctat atctcccacc gcatgaaaga aatcttcgag atttgcgatg ccgttaccgt
     3241 ttttcgtgac gggcaattta ttgctgagcg cgaagtggca tcactgaccg aagattcgct
     3301 gattgagatg atggtgggtc gcaagctgga agatcaatat ccacacctgg acaaagcgcc
     3361 gggagatatc cgcctgaaag tcgataatct ctgcggacct ggcgttaacg atgtctcttt
     3421 tactttacgc aaaggcgaaa ttcttggcgt ctctggtttg atgggcgcag gtcgtaccga
     3481 actgatgaaa gtgctctacg gcgcactacc gcgcaccagc ggttacgtca ccctggatgg
     3541 gcatgaagtc gttacccgtt caccgcagga tggcctggca aacggcattg tgtatatctc
     3601 cgaagaccgt aaacgtgacg gtttagtgtt gggcatgtca gtaaaagaga acatgtcgct
     3661 gacagcgctg cgctacttca gccgcgctgg cggcagtttg aagcatgccg atgaacagta
     3721 ggctgtgagt gatttcattc gtctgtttaa tgtgaaaaca ccgtcgatgg aacaggcaat
     3781 tggtctgctt tccggtggca atcagcaaaa agtgttgatc cttgatgaac ctacccgtgg
     3841 cgtagatgtc ggcgcgaaaa aagagatcta tcaactgatt aaccagttca aagccgatgg
     3901 cttgagcatc attctggtgt catcggagat gccagaagta ttaggcatga gcgatcgcat
     3961 catcgtcatg catgaagggc atctcagcgg ggaatttact cgtgagcagg ccacccagga
     4021 agtgttaatg gctgccgctg tgggcaagct taatcgcgtg aatcaggagt aaaaaaatga
     4081 caacccagac tgtctctggt cgccgttatt tcacgaaagc gtggctgatg gagcagaaat
     4141 cgcttatcgc tctgctggtg ctgatcgcga ttgtctcgac gttaagcccg aactttttca
     4201 ccatcaataa cttattcaat attctccagc aaacctcggt gaacgccatt atggcggtcg
     4261 ggatgacgct ggtgatcctg acttcgggca tcgacttgtc tgtaggttct ctgttggcgc
     4321 taaccggcgc agttgctgca tctatcgtcg gcattgaagt caatgcgctg gtggctgtcg
     4381 ctgctgctct cgcgttaggt gccgcaattg gtgcggtaac cggggtgatt gtagcgaaag
     4441 gtcgcgttca ggcgtttatc gctacgctgg ttatgatgct tttactgcgc ggcgtgacca
     4501 tggtttatac caacggtagc ccagtgaata ccggctttac tgagaacgcc gatctgtttg
     4561 gctggtttgg tattggtcgt ccgctgggcg taccgacgcc agtctggatc atggggattg
     4621 tcttcctcgc ggcctggtac atgctgcatc acacgcgtct ggggcgttac atctacgcgc
     4681 tgggcggcaa cgaagcggca acgcgtcttt ctggtatcaa cgtcaataaa atcaaaatca
     4741 tcgtctattc tctttgtggt ctgctggcat cgctggccgg gaccattgaa gtggcgcgtc
     4801 tctcctccgc acaacccacg gcggggactg gctatgagct ggatgctatt gctgcggtgg
     4861 ttctgggcgg tacgagtctg gcgggcggaa aaggtcgcat tgttgggacg ttgatcggcg
     4921 cattaattct tggcttcctt aataatggat tgaatttgtt aggtgtttcc tcctattacc
     4981 agatgatcgt caaagcggtg gtgattttgc tggcggtgct ggtagacaac aaaaagcagt
     5041 aataacgact acaggacatc ttgaatatga acatgaaaaa actggctacc ctggtttccg
     5101 ctgttgcgct aagcgccacc gtcagtgcga atgcgatggc aaaagacacc atcgcgctgg
     5161 tggtctccac gcttaacaac ccgttctttg tatcgctgaa agatggcgcg cagaaagagg
     5221 cggataagct tggctataac ctggtggtgc tggactccca gaacaacccg gcgaaagagc
     5281 tggcgaacgt gcaggactta accgttcgcg gcaccaaaat tctgctgatt aacccgaccg
     5341 actccgacgc agtaggtaat gctgtgaaga tggctaacca ggcgaacatc ccggttatca
     5401 ctcttgaccg ccaggcaacg aaaggtgaag tggtgagcca cattgcttct gataacgtac
     5461 tgggtggcaa aatcgccggt gattacatcg cgaagaaagc gggtgaaggt gccaaagtta
     5521 tcgagctgca aggcattgct ggtacatccg cagcccgtga acgtggcgaa ggcttccagc
     5581 aggccgttgc tgctcacaag tttaatgttc ttgccagcca gcagattttg atcgcactaa
     5641 aggtttgaac gtaatgcaga acctgttgac cgctcatccg gatgttcagg ctgtattcgc
     5701 gcagaatgat gaaatggcgc tgggcgcgct gcgcgcactg caaactgccg gtaaatcgga
     5761 tgtgatggtc gtcggatttg acggtacacc ggatggcgaa aaagcggtga atgatggcaa
     5821 actagcagcg actatcgctc agctacccga tcagattggc gcgaaaggcg tcgaaaccgc
     5881 agataaagtg ctgaaaggcg agaaagttca ggctaagtat ccggttgatc tgaaactggt
     5941 tgttaagcag tagttttaat caggttgtat gacctgatgg tgacataaat acgtcatcga
     6001 cagatgaacg tgtaatataa agaaaagcag ggcacgcgcc accctaacac ggtggcgcat
     6061 tttatggaca tcccgaatat gcaaaacgca ggcagcctcg ttgttcttgg cagcattaat
     6121 gctgaccaca ttcttaatct tcaatctttt cctactccag gcgaaaccgt aaccggtaac
     6181 cactatcagg ttgcatttgg cggcaaaggc gcgaatcagg ctgtggctgc tgggcgtagc
     6241 ggtgcgaata tcgcgtttat tgcctgtacg ggtgatgaca gcattggtga gagcgttcgc
     6301 cagcagctcg ccactgataa cattgatatt actccggtca gcgtgatcaa aggcgaatca
     6361 acaggtgtgg cgctgatttt tgttaatggc gaaggtgaga atgtcatcgg tattcatgcc
     6421 ggcgctaatg ctgccctttc cccggcgctg gtggaagcgc aacgtgagcg tattgccaac
     6481 gcgtcagcat tattaatgca gctggaatca ccactcgaaa gtgtgatggc agcggcgaaa
     6541 atcgcccatc aaaataagac tatcgttgcg cttaacccgg ctccggctcg cgaacttcct
     6601 gacgaactgc tggcgctggt ggacattatt acgccaaacg aaacggaagc agaaaagctc
     6661 accggtattc gtgttgaaaa tgatgaagat gcagcgaagg cggcgcaggt actgcatgaa
     6721 aaaggtatcc gtactgtact gattacttta ggaagtcgtg gtgtatgggc tagcgtgaat
     6781 ggtgaaggtc agcgcgttcc tggattccgg gtgcaggctg tcgataccat tgctgccgga
     6841 gataccttta acggcgcgtt aatcacggcg ttgctggaag aaaaaccatt gccagaggcg
     6901 attcggtttg cccatgctgc agctgcgatt gccgtaacac gtaaaggcgc acaaccttcc
     6961 gtaccgtggc gtgaagagat cgacgcattt ttagacaggc agaggtgacg cttggctaca
     7021 atgaaagatg ttgcccgcct ggcgggcgtt tctacctcaa cagtttctca cgttatcaat
     7081 aaagatcgct tcgtcagtga agcgattacc gccaaagttg aagcggcgat taaagaactc
     7141 aattacgcgc catcagccct ggcgcgtagc ctcaaactca atcaaacaca taccattggc
     7201 atgttgatca ctgccagtac caatcctttc tattcagaac tggtgcgtgg cgttgaacgc
     7261 agctgcttcg aacgcggtta tagtctcgtc ctttgcaata ccgaaggcga tgaacagcgg
     7321 atgaatcgca atctggaaac gctgatgcaa aaacgcgttg atggcttgct gttactgtgc
     7381 accgaaacgc atcaaccttc gcgtgaaatc atgcaacgtt atccgacagt gcctactgtg
     7441 atgatggact gggctccgtt cgatggcgac agcgatctta ttcaggataa ctcgttgctg
     7501 ggcggagact tagcaacgca atatctgatc gataaaggtc atacccgtat cgcctgtatt
     7561 accggtccgc tggataaaac tccggcgcgc ctgcggttgg aaggttatcg ggcggcgatg
     7621 aaacgtgcgg gtctcaacat tcctgatggc tatgaagtca ctggtgattt tgaatttaac
     7681 ggcgggtttg acgccatgcg ccaactgtta tcacatccgc tgcgtcctca ggccgtcttt
     7741 accggaaatg acgctatggc tgttggcgtt taccaggcgt tatatcaggc agagttacag
     7801 gttccgcagg atatcgcggt gattggctat gacgatatcg aactggcaag ctttatgacg
     7861 ccaccattaa ccactatcca ccaaccgaaa gatgaactgg gggagctggc gattgatgta
     7921 ctcatccatc ggataaccca gccgaccctt cagcaacaac gattacaact tactccgatt
     7981 ctgatggaac gcggttcggc ttagatttac gctgtctttt gatcaaatta ttaccatcgg
     8041 ttgttttcag aagcatgaac attgctgctg aagcaacagt aataatgccc atcgtgataa
     8101 acgtatagtg gaattgttcg acagtcgttg tgccttccat cccttcataa acgcgaagga
     8161 cggccgcact tacagcaacg cctaaactaa tcgacagttg ctgcgtgacc gccagaacac
     8221 tgttaccgct gctggcgttg tcatcggtca gatcggcaag tgtgatggta ttcatcgcgg
     8281 taaattgcgt cgacatagcc atccctaata taaacaacgg caagatcagc atccatatcg
     8341 ccattgccgg tgattgcaaa gagaactgag cgatcattag cccaataatc accgtgatcc
     8401 ccactaacgt atggcgatag cccagacgac gtaagacttg ggtaaccatc gattttgcaa
     8461 taatggaacc taacgctgtc ggtgccatca tacagccggc aataaacgcc tgataaccaa
     8521 atcctacctg taacatcaat ggcataagga acggtacact accggtcccc agacgggttg
     8581 caatattgcc tacgataccg atcgagaaag tgcgggtttt aaataaatct aatgaaatta
     8641 atgggtttgg cgtgtgtcgc gcatggagaa tatagagaag cagtaacccg atgctggtga
     8701 caattaccgt caaggcaatc cagctggcga caatcttttc cccgaatagc tctattccgc
     8761 ttgagaagag aacaaggctg aggccaaaca gcaaaaagcc agtgatatcg aatctgcgtc
     8821 gtgcggtggt gaaattgggc atatgtttgc gcgcgtaaag aaggcccgca atacctatgg
     8881 ggatattgat taaaaatatc cagtgccagg ttgccctggt gaccagcacg ccgccaagaa
     8941 cggggcctaa aattggcccc accagacccg gcatggcgac aaaattcaat acaggaagaa
     9001 gttcattacg aggataagca cgcagtaagg ccagccgagc aacaggcatc atcattgcgc
     9061 cgcctatccc ctgaataacc cggaagacaa ccagctgtgg tagcgaatta gaaagtgcgc
     9121 aggccagaga acccaacgtg aacagactca cggcaagggt aaaaatgcga cgcgtaccga
     9181 agcgatcggc tagccatccg cttaccggaa taaacatcgc caccgtcagc gtataactga
     9241 tgatggctga ttgcatcgcg agaggagaac gattaaggct atgagcgatt gcgggtaagg
     9301 cggtattcag aatagtggca tcaagtgcct gcatgaagaa ggccatcgcc gcgatccacg
     9361 gcaaacccgc catactacgc ttctttttat cgctcattca atgtcctgtt atcgggttat
     9421 cacttatcag gtgagcgtag cagcgcctga caagttttaa atgccgcgtc gccatcgctt
     9481 tggataatcg catcgacaat cgcctggtgc agatccagct ttatcactgt gtcgctggta
     9541 attgacgtga agtaagtgtg ataaaccgaa tggaatagcg aggcgaatga ggtcaaaaac
     9601 ggattggcgc tcatttcata gatatgctca tgccaggcca tatcgacttc gatccagcgt
     9661 tcacggcgaa agttcttttt taatgccgcc atttcggcca ttaacgtatt gagatgcgcc
     9721 ttctgttccg cggtgccaac cgttgctgcc agtaggcagg cttgcggctc cagacagata
     9781 cgcataacca gaaagtgatc gatgacctga tgaaagttct cttctgtcat ccaccaggta
     9841 agcaattcct gatcaagaaa attccagttt gattgtggca tgacccgggt accaattcgc
     9901 ggtcgcggta aaaccatccc ttttgccgtt aacgttttga ccgcttcgcg taccgctgta
     9961 cgactcactc caaattgctc gcccagctca atctcaccag gcaaaatggt gccgggttca
    10021 tattcacctt ttaagatgcg ttgcgccagc ttctcagcca gaacatacga aaggtttttc
    10081 tgtgcagcta actgttgtgc gcttaaaggc attactaatc ttcctttttc tttttattcc
    10141 tccttagtat gccaccagga agtgtgatta cggttgcaaa aacggcaaat tgcttgtttt
    10201 atggcacatt aacggggctt ttgctgaaaa agtgcgcggt cagaaaatta ttttaaattt
    10261 cctcttgtca ggccggaata actccctata atgcgccacc actgacacgg aacaacggca
    10321 aacacgccgc cgggtcagcg gggttctcct gagaattccg acagagaaag caaaaataaa
    10381 tgcttgactc tgtagcggga aagcgtatta tgcacacccc gcgccgctga gaaaaagcga
    10441 agcggcactg ctctttaaca atttatcaga caatctgtgt gggtactcga agatacggat
    10501 tcttaacgtc gcaagacgat aaatg
//
LOCUS       DAZPEB010000143        10480 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZPEB010000143 DAZPEB010000000
VERSION     DAZPEB010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10480
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.146"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(37..1032)
                     /gene="gapA"
                     /locus_tag="ACHURJ_003109"
     CDS             complement(37..1032)
                     /gene="gapA"
                     /locus_tag="ACHURJ_003109"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFR1281958.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            1374..1787
                     /gene="msrB"
                     /locus_tag="ACHURJ_003110"
     CDS             1374..1787
                     /gene="msrB"
                     /locus_tag="ACHURJ_003110"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFR1281959.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            1829..2101
                     /locus_tag="ACHURJ_003111"
     CDS             1829..2101
                     /locus_tag="ACHURJ_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFR1281960.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(2262..2903)
                     /gene="pncA"
                     /locus_tag="ACHURJ_003112"
     CDS             complement(2262..2903)
                     /gene="pncA"
                     /locus_tag="ACHURJ_003112"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFR1281961.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(2914..3930)
                     /gene="ansA"
                     /locus_tag="ACHURJ_003113"
     CDS             complement(2914..3930)
                     /gene="ansA"
                     /locus_tag="ACHURJ_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFR1281962.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPDDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(4097..5953)
                     /gene="sppA"
                     /locus_tag="ACHURJ_003114"
     CDS             complement(4097..5953)
                     /gene="sppA"
                     /locus_tag="ACHURJ_003114"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFR1281963.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDHNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPSAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            6114..6665
                     /locus_tag="ACHURJ_003115"
     CDS             6114..6665
                     /locus_tag="ACHURJ_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFR1281964.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            6782..7825
                     /gene="selD"
                     /locus_tag="ACHURJ_003116"
     CDS             6782..7825
                     /gene="selD"
                     /locus_tag="ACHURJ_003116"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFR1281965.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            7830..9791
                     /gene="topB"
                     /locus_tag="ACHURJ_003117"
     CDS             7830..9791
                     /gene="topB"
                     /locus_tag="ACHURJ_003117"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFR1281966.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
     gene            9918..10373
                     /locus_tag="ACHURJ_003118"
                     /pseudo
     CDS             9918..10373
                     /locus_tag="ACHURJ_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416276.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10390..>10480)
                     /locus_tag="ACHURJ_003119"
     CDS             complement(10390..>10480)
                     /locus_tag="ACHURJ_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR1281967.1"
                     /translation="LGRKSLSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT         2637 a         2561 c         2760 g         2522 t
ORIGIN      
        1 gaagtcgctc tttttagatc acagtttcat ctcaacttat ttggagatgt gagcgatcag
       61 gtccagaact ttgttggagt aaccggtttc gttgtcgtac caggatacca gtttcacgaa
      121 gttgtcgttc agagcgatac cagctttagc atcgaacacg gaagtgcaaa cttcgccgtt
      181 gaaatcggta gatactacgt catcttcggt gtagcccaga acgcctttca tttcgccttc
      241 agcagcggct ttaacggcag ctttgatctg ctcgtaagtt gcagcttttt ccagacgaac
      301 ggtcaggtca actacggata cgttcggggt cggaacgcgg aacgccatac cagtcagttt
      361 gccattcagt tctggcagta ctttacctac agctttagca gcaccggtag aggacgggat
      421 gatgttctgg gaagcgccgc ggccgccgcg ccagtctttg tgagacgggc catcaacggt
      481 tttctgagta gcggtagtag cgtgaacggt ggtcatcaga ccttcgatga tgccgaagtt
      541 atcgttgata actttagcca gcggagccag gcagttggtg gtgcaggaag cgttggaaac
      601 gatgtcctgg ccagcatatt tgtcgaagtt agcgccttta acgaacatcg gagtgttgtc
      661 tttagacgga ccagtcataa ccactttctt cgcaccagcg gtgatgtgtt tacgagcagt
      721 ttcgtcagtc aggaacagac cagttgcttc agcgacaacg tcaacaccaa cttcgtccca
      781 tttcaggtta gccggatcac gttcagcggt aacacggatt tttttaccgt taacgatcag
      841 atgaccgtct ttcacttcaa cggtaccgtc gaaacggccg tgagtggagt catatttcag
      901 catgtatgcc atgtaatcag cgtctaacag gtcgttgatt gcaacgatct cgatgtcaga
      961 acgtttctga gcagcacgga aaacaatgcg accgatacgg ccaaaaccgt tgatacctac
     1021 tttgatagtc atatattcca ccagctattt gttagtgaat aaaaggttgc ctgtaaaatt
     1081 acaaaaacct tacgcagcgt caagcggaat cgtgtcaatc attgcgacaa atcaatcctg
     1141 tgcctaagca ttatgcgact gactcgcctc actcttcctt tgggcttgaa accacatggg
     1201 gtcggcgccc cgaattttaa agggcaatta cgataaaaat gtgattttca tcacgattta
     1261 ggtgcagcca tttcgtcgag atcaagttta ggacaaaatt ttgccagttg gtgttaatgt
     1321 tttgttagaa tcagtcacgt aatgtgagca cgattaaagt gagatgtgag caaatggcta
     1381 ataaaccttc ggcagaagaa ctgaaaaaaa atttgtccga gatgcagttt tacgtgacgc
     1441 agaatcatgg gacagagccg ccatttacgg ggcgtttact gcataacaag cgtgacggcg
     1501 tatatcactg tttgatctgc gatgccccgc tgtttcattc cgaaaccaag tatgattccg
     1561 gctgtggctg gcccagtttc tacgaaccgg tgagtgaaga atccattcgt tatatcaaag
     1621 acttgtcaca tggaatgcag cgcatagaaa ttcgttgcgg taactgtgat gcccatctgg
     1681 ggcatgtctt tcccgacggg ccgcaaccaa caggcgaacg ttattgtgtt aactctgctt
     1741 ctttacgctt cactgatggc gaaaacggcg aagaaatcaa cggttgaaaa aacgattcag
     1801 caacttattc cacaggagcg gaaaaattat gaatcttgat gacattatca acagcatgac
     1861 gcctgaggta taccagcgtt tgtcgaccgc cgttgaactg gggaaatggc ctgatggcgt
     1921 tgcgttgacc gaggaacaaa aagagaattg cctgcaactg gtgatgctgt ggcaagcccg
     1981 ccataatacc gaagcacagc atatgacaat tgacacaaat ggtcagatgg tcatgaagag
     2041 caaacagcag ttaaaagaag attttggtat ctcagcaaag cctattgcta tgtttaagtg
     2101 aatgagttat ctagtattta agcctgttat taagggaggg caaatatact tgttcggacg
     2161 tcgtgtttta gctgcaagta ggttaaatgc aacactgaca agtattatct ggcctacaat
     2221 gtcattgagg aaatgtaggc cagagatgaa ttcaactaat attacccctg tgtctcttcc
     2281 cagtctgcca gcgtatatag cgttgcccca gctgctgaca tctccataaa cgcgtgtgca
     2341 ctgtcctggg gctggatatt cacgccacga caaccatcgg taatcacgtt caccttataa
     2401 cctaactgta acgcgtccag cacggtaaac ttcacgcaat agtcagtagc caggcccata
     2461 acgatcaatt catcgatttc atgatcgcgt aaccagtcat cgagagaggt tttctgccga
     2521 cggccgttat caaaaaaggc actgtaactg tcaactaaag gattttcgcc tttatggaac
     2581 accgctgcga tcgctttttg gttcagtaac ggatgtaatt gtgcgccttc actgttctgc
     2641 acacagtgat ctggccagaa ggtttgtggc aaaccgtcga gttggcctgg cgtataaggc
     2701 tctacaccgt gctgactggc aaaactgccg tgattcgccg gatgccagtc ctggctggcg
     2761 ataaccgctt ctccgcgcga ctggcaccag tcaataaggc ggttagcaac ctccaccgta
     2821 ctgtcacctt ccggtacggc gagcgcgcca ccagcacaga aatcattttg taaatcgacc
     2881 agtaacaggg cgcgatgggg cattgcagtc tccttaatca tccggcgtca gttcgccgcg
     2941 caggttttgg ctcatggcct tgcgaatggt ttcagtatcc agttcctggc tcagcaggta
     3001 atgcagtttg gttagcgtgg cttctacagt catatctgca ccgccaataa cgccggcatg
     3061 ggcgagggcg ttaccggtgg cataaccacc catattcact ttaccggaca tacattgtgt
     3121 caggttgacc accacaatac cgcgatcgct ggcttcttgt aattcctgca ggaaggcttt
     3181 gttttgtggc gcattaccca cgccatagga gcgcagaatc aatgctttca ctggttggcg
     3241 cagaaaattg cgcacgacat cagcagaaat ccccggataa atcgtcacta cgccaattgg
     3301 ttgtggcgtg attggatgaa cgattaattc cccttcaccg tgcggggcgg gtggcgtatt
     3361 caaacgacga atatggatac cagcttccag taacggagga aggtttggag aggcaaacgc
     3421 atcaaaacca tcggcatggg ctttggtagt gcggttgccg cgatacaatc ggttattgaa
     3481 aaagagcgtt acttcgttga tcggataatt cgccgcaacg tacaacgcat tcagcagatt
     3541 aatttgtccg tcagagcgta actcagccag cgggatttgt gaccctgtca caatgaccgg
     3601 tttaccgaga ttctcgagca tgaacgacag cgcagaggcg gtatacgcca tcgtgtcggt
     3661 accgtgtaga atgacaaaac catcatagtc gtcatagtgc gctttaatat cttcagcaat
     3721 atgctgccag tcatccggcg tcatatcaga agaatccatc aacggcgtat attcatgaat
     3781 ggtgaaatct ggcatctccg ggcgatggaa ttccggcatc agcgccagtt ggcgttgtag
     3841 atgacctgac accggaatat aaccctgctc ggaacgctgc atcccgatgg tcccgcccgt
     3901 gtaggcgacg taaattgatt tcttttgcat gatattgata tgaatgatat cgaaagagca
     3961 aaagtatata cgtgaggcag gaggagcgca cctgatacta tgcggataag gaaatatggg
     4021 gcgagacagc gggaagagga ttcgtcgagg taaaaaataa tgtggccgac gatctgtcgg
     4081 ccactcagta caagacttaa cgcacgttgg cgcaggtcag gcaaaacgca taacggtttt
     4141 gtgggtcgtt aaacgcggcc agcttgtcgc tttcactttt aacggtagag gctaccgagg
     4201 caagcggtgc aggtaacatg gcctggaacg catctggcaa cattgcccgg acagaaccag
     4261 acatgttgtc catcactttg tcgaagaagg tcggttcatc aacgtagtat tccagatgcc
     4321 actgtttcac ttttgcgagc tctgctgctt tggcaaccgc atcatcgaaa tccccgagac
     4381 tatcgaccag cccgttagct tttgcatcct gaccggtcca gacgtggccc tgggcgattt
     4441 tatcaatttg ctccggcgtc gaatgacgcg catcagcaac cagcgtgata aagcgtttat
     4501 agccattctc aatgcttaat tgcatcatct gctgcgcttc cggcggcagt gccctggtga
     4561 tagaaacatc cgccagcggt gaagttgaga caccatcagt atgaacacca atcgaatcca
     4621 gactattttc tacggtggtg atcacgccga agataccgat agaaccggtc agggtgctgg
     4681 ggttagccac aatgtaatta gctggcgtgg aaatccagta accaccagat gccgccatgc
     4741 cgcccatcga tacaaccaca ggcttacccg ctgcccgggc tgctgccagt tcagcgcgaa
     4801 tcacttcaga cgcggtaacg ctgccgcctg ggctattaac acgcaggaca atcgctttca
     4861 ctttcgggtc aaggcgagcg tcgcggattt gtgccgcagt ggtatcaccg ccaacattcc
     4921 cctgagtttc ctcgccatcc ataattgcgc cattagcgaa gacgacaccg atgctgtcac
     4981 cggtatctgc cggcgttttc aatgcgtaat cgtaataact gatggcgcga taatttttat
     5041 cagtcttact ccagccgaac tctttggtca gagctttttc gatttcggca ctcgatgcca
     5101 gtgcatcgac cagcttgttt tccagtgcat atttcgcggt atcgccaccg gttttggtta
     5161 aaccctcaag caacccttgc gcgctaggga atacctgctg agcagggatc tgccggttag
     5221 cggcaacagt attcagatag ttttgccaca gctcaccaat ccagcggctg tcagcttcgc
     5281 gggctgccgg tgacatatca tcacgaataa acggttcaac ggcagattta tacgtaccca
     5341 cgcggaacac atgggtggaa actttcagct tatccagcaa cgatttgtag tacagaccgt
     5401 tggtagcaaa accgtgcaga tcaaccacgc cttgcggtga cagccaaatt ttattggcga
     5461 aactggcgag ataatattgc ccctggctgt agttctcgcc aacggcataa accggtttcc
     5521 cgctgtcacg aaactctttc agggctttgc cgatgtactg catagacggc tggtcgccgc
     5581 ctgcgaagtt tttcagatcc atcacgatac cggtgatatt gtggtcgtcc ttcgcctggc
     5641 gaatggtgtt gacgatatca aacagtgagt tttcctgcag acgatcggaa ctggcaccaa
     5701 gcagctggcg gcttaattta ctaaaccgct gagaactgtc gggtttatcg acgatcacac
     5761 cggaaatgtc cagcagcagt gcgccacgac tggccgtttc tttcgaatca ccaccactga
     5821 cctgcatcca gatccccaca ccaaccagta cgaggaaaat aaagaacagg ttaagtacca
     5881 tttcacggac gaaattcagc agacgccacg tccatttaaa aaatccggca ataaatcgcc
     5941 aaagggttcg catgtattct cccaacttaa ggtcacacct gtcacaggcg caatagatgt
     6001 ggttatcgta atgacccaac cgacacttgt cagcaggaat tgcggactgt gctgtaacaa
     6061 aatccactct cgtgttaatt ttgtgaataa atatcacgac aggagttaat caaatggatg
     6121 cactcgaact attgatcaat cgccgtagcg cctcccgcct ggctgaaccc gcgccaacgg
     6181 gtgaacaact gcaaaacatc ctgcgtgcgg gtatgcgtgc gccggaccat aagtccatgc
     6241 aaccgtggca tttttttgtg attgaagggg aagggcgcga gcgtttcagc gccgtactgg
     6301 aacagggggc gattgctgcc ggtagtgatg acaaagctat cgacaaagcc cgtaatgcgc
     6361 cgttccgcgc accgctcatc atcacggtgg tggcgaaatg cgaagaaaat cataaagtcc
     6421 cgcgatggga acaggaaatg tctgccggat gcgcggtcat ggcgatgcaa atggcagccg
     6481 ttgcccaggg atttggcggc atctggcgca gtggcgcatt aactgaaagt ccggtagtgc
     6541 gtgaagcatt cggttgccgt gagcaggata aaattgtcgg ttttctctac ctcggcacgc
     6601 cgcagttgaa agcatctacg tcgattaacg tcccggaccc gacgccgttt gtaacttatt
     6661 tctgataatt gtcgcgaaac tgtctggatt gtgagcaaag gcgcaggaat tcagacactc
     6721 tcacttatca cttcacggaa tgagggttac catacccgga ttgcattgac aggagatgtc
     6781 catgagcgag aactcgattc gtttgaccca atacagccac ggagctggtt gcggctgtaa
     6841 aatttcccca aaagtgttgg aaaccatcct gcacagtgag caggcgaagt ttgttgatcc
     6901 gaatttgctt gtgggtaatg agacccgcga cgatgcggcg gtgtacgatc tgggcaatgg
     6961 caccagcgtt atcagtacca ccgacttctt tatgccgatc gttgataatc ctttcgattt
     7021 tggccgcatt gcggcgacta acgccatcag cgatatcttc gcgatggggg gcaaaccgat
     7081 tatggcgatt gcgatcctcg gctggccgat taacaaactt tccccggaaa ttgcccgcga
     7141 agtaaccgaa ggtggacgct atgcatgccg tcaggcgggt attgcgctgg ctggcggtca
     7201 ctccatcgat gcgccggagc cgatttttgg tctggcggta acggggatcg taccgaccga
     7261 gcgggtgaag aaaaacagca ccgcacaagc cggatgcaaa ctgttcctga cgaaaccgct
     7321 ggggatcggc gttcttacca cggctgagaa aaaatcattg ctgaaaccag aacatcaggg
     7381 actagcgacg gaagtgatgt gccggatgaa catcgcaggc gcgtcctttg ccaacatcga
     7441 aggcgtaaaa gcgatgaccg acgttacggg ctttggtctg ctgggccatt tgagcgaaat
     7501 gtgtcagggc gcaggtgtgc aggcgcgcgt tgactatgac gcgatcccaa aattgccggg
     7561 ggttgaagag tacattaagt tgggcgcagt gcctggcggc accgaacgta actttgccag
     7621 ctacggtcat ctgatgggtg aaatgccgcg tgaagtgcgc gatttgctgt gcgatccgca
     7681 aacttctggc ggtttgctgc tggcggtcat gccggaagca gaaaatgagg tcaaagctac
     7741 agccgccgag tttggcattg aactgacggc aattggcgaa ctggtgccag cgcgcggcgg
     7801 tcgtgccatg gttgagattc gttaattcaa tgcggttgtt tattgccgaa aaaccgagtc
     7861 tggcgcgcgc cattgctgat gtcctgccca aaccgcaccg gaaaggcgat ggctttatcg
     7921 agtgcggtaa tggtcaggtg gtgacctggt gtatcggtca cctgcttgag caggcgcagc
     7981 cagacgccta cgacagccgc tatgcgcgct ggaatcttgc ggatttgccg attgtcccgg
     8041 aaaagtggca attacagccc cgaccctccg tgaccaaaca acttaacgtc atcaaacgct
     8101 tcctgcatga agccagcgaa atcgttcacg ccggggaccc ggatcgtgaa gggcaactgc
     8161 tggtggatga agtgctggac tatctgcaac tggcaccgga aaagcgccag caggtgcagc
     8221 gttgcttgat aaacgacctg aacccgcagg cggttgagcg ggcgatcgac cgtcttcggt
     8281 ccaacagtga gtttgtgcca ttgtgcgttt ctgcgctggc gcgagcgcgt gccgactggc
     8341 tgtacggcat caatatgacc cgtgcgtata ccattctcgg tcgcaatgcc ggttatcagg
     8401 gcgtactttc cgtgggacgc gtgcagacac ccgtgctcgg gctggtggtg cgccgcgatg
     8461 aagagattga aaacttcgtg gcgaaagact tctttgaagt caaagcacat atcgtgacac
     8521 ctgccgatga gcggtttacc gctatctggc aaccgagcga agcgtgtgaa ccgtaccagg
     8581 atgaagaagg gcgcttgtta catcgtccac tggcggagca tgtggttaac cgcattagtg
     8641 gtcaaccagc aattgtcacc agctataacg ataaacggga atcagaatcc gcgccgctgc
     8701 ctttttcgct ttcggcgttg cagattgaag cggcaaaacg ctttggtctg agcgcgcaga
     8761 acgtgcttga tatctgccag aagctgtacg aaacgcacaa gctaatcact tatccgcgtt
     8821 ccgattgtcg ctatttgcca gaagaacatt ttgccggacg ccacgcggtg atgaatgcca
     8881 tcagcgttca tgcaccagat ctgttgccgc aaccagtggt agatccagat atacgcaacc
     8941 gctgttggga tgacaaaaag gtcgatgcac accacgccat catcccgacc gcacggagtt
     9001 ctgcgatcaa cctgacggag aacgaagcga aggtctataa cctgattgcc cgtcagtact
     9061 tgatgcagtt ctgcccggat gcggtgttcc gcaagtgtgt tatcgaactg gacattgcca
     9121 aaggcaaatt tgtcgctaaa gcgcgttttc ttgctgaagc aggctggcgc acgctgttag
     9181 gcagcaaaga gcgcgatgaa gaaaacgacg gtacgccatt gcctgtggtg gcgaaaggcg
     9241 atgagttgct gtgtgaaaaa ggtgaagtgg tagagcggca aacccagccg ccgcgccatt
     9301 ttaccgatgc aacactgctt tcggcgatga ccgggatcgc gcgctttgtg caggacaaag
     9361 atctgaaaaa gatccttcgt gcgaccgatg gtctggggac agaagcaacc cgtgccggga
     9421 ttattgaact gctgttcaag cgtggttttc tgaccaaaaa agggcgctat atccactcta
     9481 ccgacgccgg aaaagcgtta ttccattcgc tgccagaaat ggcgacgcga ccggacatga
     9541 ccgcgcactg ggaatcggtg ctgacgcaaa tcagcgaaaa gcagtgtcgc tatcaggact
     9601 ttatgcagcc gctggtgggg acgctatatc agcttattga tcaagccaaa cgtacgccgg
     9661 tgcggcagtt tcgcggcatt gtggctccgg gcagtggtgg cagtgctgat aagaaaaagg
     9721 ctgcaccgcg taaacgtagt gcgaaaaaaa gtccgccagc agatgaagtc ggaagcgggg
     9781 cgatagcgta agcgagtgaa tctttcgtgc tattcgagtc atattctgaa atatccagcg
     9841 aatcaagaaa attcgttgga tatttttttg catggataaa attatcgcct ctaaagtatg
     9901 taataacagg gaatgtgatg aaaaaagttt tattacaaaa ccatcctggg agcgagaagt
     9961 attcttttaa tggctgggaa atatttaata gtaattttga acggatgatt aaagaaaata
    10021 aggccatgct gctttgtaag tgggggtttt atttaacatg tgttgtcgct gtaatgtttg
    10081 tgttcgcagc gataacatcc aacggtttga atgaaagagg cctgattacc gcgggatgct
    10141 cttttcttta tctattaatt atgatggggc ttattgttcg ggccggtttt aaagcaaaaa
    10201 aagaacaact gcattattat caggctaaag gtattgagcc gctcagtatc gagaagttac
    10261 aggcgctaca attgatcaca ccttatcgat tctatcataa gcaatggtct gaaacgctgg
    10321 agttctggcc gcgaaagcct gaacctggca aagatacctt ccaatatcat gtgcggtaat
    10381 gactccaact tactgatagt gttttatgtt cagataatgc ccgatgacct tgtcatgcag
    10441 ctccaccgat tttgagaacg acagtgactt ccgtcccagc
//
LOCUS       DAZPEB010000144        10458 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZPEB010000144 DAZPEB010000000
VERSION     DAZPEB010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10458
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.147"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            252..1934
                     /locus_tag="ACHURJ_003120"
     CDS             252..1934
                     /locus_tag="ACHURJ_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFR1281968.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYNLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1986..3143
                     /locus_tag="ACHURJ_003121"
     CDS             1986..3143
                     /locus_tag="ACHURJ_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFR1281969.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CSFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(3109..3231)
                     /locus_tag="ACHURJ_003122"
     CDS             complement(3109..3231)
                     /locus_tag="ACHURJ_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1281970.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            3446..5131
                     /locus_tag="ACHURJ_003123"
     CDS             3446..5131
                     /locus_tag="ACHURJ_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFR1281971.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELRDNFHTIKENWHRLMNRQR
                     LLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVANASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDIFSPADSWYVSQTPLIKTGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISAVL"
     gene            5169..7541
                     /locus_tag="ACHURJ_003124"
     CDS             5169..7541
                     /locus_tag="ACHURJ_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFR1281972.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVVGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYSGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            7598..10381
                     /locus_tag="ACHURJ_003125"
     CDS             7598..10381
                     /locus_tag="ACHURJ_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFR1281973.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKKALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
BASE COUNT         3079 a         2294 c         2264 g         2821 t
ORIGIN      
        1 tgattattta tgttctcaat tcagcatttt gtcagtaaaa tgcaataatt tattaaatat
       61 caataaatta gttgtttatc ggcgagaaat tacttaatag aacagaaagt aatgtcaacg
      121 ctttatggac tgttatttcc ctttttttag ctaaatctgc tatctcttta tgtgactaac
      181 ttcacttaca tccacttatt tctcttcgta aaattacttc ggaattaagt acaataagaa
      241 gaggaacatt tatgaagtct gcattaaaga aaagtgtcgt aagtacctcg atatctttga
      301 tactggcatc tggtatggct gcatttgctg ctcatgcggc agatgatgta aagctgaaag
      361 caaccaaaac aaacgttgct ttctcagact ttacgccgac tgaatacagt accaaaggaa
      421 agccaaatat tatcgtacta accatggatg atcttggtta tggacaactt ccttttgata
      481 agggatcttt tgacccaaaa acaatggaaa atcgtgaagt tgtcgatacc tacaaaatag
      541 ggatagataa agccattgaa gctgcacaaa aatcaacgcc gacgctcctt tcattaatgg
      601 atgaaggcgt acgttttact aacggctatg tggcacacgg tgtttccggc ccctcccgtg
      661 ccgcaataat gaccggtcga gctcccgccc gctttggtgt ctattccaat accgatgctc
      721 aggatggtat tccgctaaca gaaactttct tgcctgaatt attccagaat catggttatt
      781 acactgcagc agtaggtaaa tggcacttgt caaaaatcag taatgtgccg gtaccggaag
      841 ataaacaaac gcgtgactat catgacaact tcaccacatt ttctgcggaa gaatggcaac
      901 ctcaaaaccg tggctttgat tactttatgg gattccacgc tgcaggaacg gcatattaca
      961 actccccttc actgttcaaa aatcgtgaac gtgtccccgc aaaaggttat atcagcgatc
     1021 agttaaccga tgaggcaatt ggcgttgttg atcgtgccaa aacacttgac cagcctttta
     1081 tgctttacct ggcttataat gctccgcacc tgccaaatga taatcctgca ccggatcaat
     1141 atcagaagca atttaatacc ggtagtcaaa cagcagataa ctactacgct tccgtttatt
     1201 ctgttgatca gggtgtaaaa cgcattctcg aacaactgaa gaaaaacgga cagtatgata
     1261 atactattat tctctttacc tccgataatg gtgcggttat cgatggtcct ctgccgctga
     1321 acggggcgca aaaaggctat aagagtcaga cctatcctgg cggtactcac accccaatgt
     1381 ttatgtggtg gaaaggaaaa cttcaacccg gtaattatga caagctgatt tccgcaatgg
     1441 atttctaccc gacagctctt gatgcagccg atatcagcat tccaaaagac cttaagctgg
     1501 atggcgtttc cttgctgccc tggttgcaag ataagaaaca aggcgagcca cataaaaatc
     1561 tgacctggat aacctcttat tctcactggt ttgacgagga aaatattcca ttctgggata
     1621 attaccacaa atttgttcgc catcagtcag acgattaccc gcataacccc aacactgagg
     1681 acttaagcca attctcttat acggtgagaa ataacgatta ttcgcttgtc tatacagtag
     1741 aaaacaatca gttaggtctc tacaatctga cggatctaca gcaaaaagat aaccttgccg
     1801 ccgccaatcc gcaggtcgtt aaagagatgc aaggcgtggt aagagagttt atcgacagca
     1861 gccagccacc gcttagcgag gtaaatcagg agaagtttaa caatatcaag aaagcactaa
     1921 gcgaagcgaa ataactaaac cttcatgcgg cggaattttc cgccgcctta ttgagcgaga
     1981 tagcgatgca cgttacagcc aagccctcca gttttcaatg taatctcaaa tgtgattact
     2041 gtttttacct tgaaaaagag tcgcagttta ctcatgaaaa atggatggat gacagcactc
     2101 tgaaagagtt catcaaacaa tatatcgcag cgtctggcaa tcaggtctat tttacctggc
     2161 aaggcggtga acccactctg gctggcctgg attttttccg taaagttatt cactatcaac
     2221 aacgctatgc aggccaaaaa cgtattttta atgcattaca aacgaatggc attttattga
     2281 ataatgaatg gtgttccttt ctcaaagaac atgaatttct ggttggtatc tcgatcgatg
     2341 gcccccagga gttacatgac cgttacagac gcagtaattc aggtaacggt acttttgcaa
     2401 aagtgatagc agccatcgag cgtctgaaat catatcaagt tgagtttaat acgttaaccg
     2461 tcattaataa cgttaatgtc cattaccctc ttgaggttta tcatttttta aaatctatcg
     2521 gcagtaaaca tatgcaattt atcgaattgc tcgaaaccgg gacgccgaat attgatttca
     2581 gtggtcatag tgagaacaca ttccgtatca ttgatttttc tgtgcctccc acggcttatg
     2641 gcaagtttat gtcaaccatt tttatgcaat gggttaaaaa cgatgtgggt gaaattttca
     2701 ttcgtcagtt tgaaagcttt gtcagccgtt ttttggggaa tgggcataca agttgtattt
     2761 tccaggagtc ctgcaaggat aatctggttg ttgaaagtaa tggagacatt tacgaatgcg
     2821 accattttgt ctatccacag tacaaaattg gaaacattaa taaatctgaa ctcaaaacga
     2881 tgaacagtgt acaactgaca gcgcaaaaaa aacggatttc agcgaaatgt cagcaatgtg
     2941 catataaacc tatctgcaat ggcggttgtc ctaagcatcg tattactaaa gtaaacaatg
     3001 agactgtttc ttatttttgc gaaggttata aaatcctttt ttcaaccatg gtaccttata
     3061 tgaacgccat ggttgagtta gctaagaaca gagtaccgct ttaccacatt atggatgttg
     3121 caaaacaaat ggagaataat taataacgct ctcttttaac tccattctta gcctccttct
     3181 tatgacctta acaaggcacc cgacataaat tcctgtcggg tgtttcgtca tcatcatctt
     3241 cccttgcgaa aatcctggct tacgtggtat gcgtcagggc attagcaata cccttgatta
     3301 tcaatagcat aaccataacc ataaccataa ccactgaaaa ttaaaaattt ctatatattt
     3361 catattgtta aatgatactt aaataaattt catcagtaaa tcattgccat agataatcat
     3421 tggatggcac aatcaatttt cgctaatgat aaccattccc attacgtttt gtatgttgat
     3481 agcaaagtat ctctgtctgc tgaagccctt ttggctgcgt aagaataata aaacttcagt
     3541 gttgttaatc attattatcc tcgcaatgat ccttggcgtg gttaaaattc aggtctggtt
     3601 aaacgactgg aataatgatt ttttcaatgc gctaagccag aaagaaaccg ataagctttg
     3661 gcagcttgtt ctttggttcc ctgccctgct ggggatcttt gtattaatat ccgtaaacaa
     3721 aacatggtta attaaactac tgactattcg ctggcgcgaa tggttaacag attactacct
     3781 taatcgctgg tttgcagata aaaactacta cttcacgcag atctacggcg agcataaaaa
     3841 tacagataac cctgaccaac gtatcgctga agacattctc ttactgatca gcaaaacgct
     3901 cagtctttca tttggcttca tccagtcact tagcatgctg atcaccttta ccgttattct
     3961 ttggcaaagc gcgggtacgc tctcattcac tgttggtgga acagaatgga atatccaggg
     4021 gtatatggtc tataccgtcg tgctcattgt gatcggtgga actttattta cccataaggt
     4081 tggtaagcgt attcgtccgc ttaatgtgga aaaacaacgt agcgaagcga catttcgaac
     4141 taatcttgtg cagcataata agcaggcaga actgattgcg ctaagcaatg ctgagtctct
     4201 acaacgccag gagttgcgcg acaattttca taccattaaa gagaactggc atcgtttaat
     4261 gaatcgtcag cggttgcttg attactggca aaatatttat tcgcgctcgc ttagcgttct
     4321 tccctacttt ctgttattgc cgcagtttat tagcgggcag atcaatctgg gcggactgat
     4381 gaaatcgcgc caggcattta tgctagtatc gaacaattta agctggttta tttataaata
     4441 tgacgaactt gctgaactgg ctgcggttat cgatcgcttg tatgagttcc atcaactcac
     4501 tgaacagcgc cctacgaata agcctaaaaa ttgccaacat gcggtacaag tggctaatgc
     4561 gagtattcgt acgcctgata ataagatcat attagagaac ttgaactttc atgtttcgcc
     4621 aggcaaatgg ctattactga aaggctactc tggtgcggga aaaaccacac tgcttaaaac
     4681 attatcccac tgctggccgt ggtttaaagg tgatattttt tctcctgctg acagttggta
     4741 tgtgtcacaa acaccgttaa tcaaaaccgg cttactgaaa gagattattt gtaaagcact
     4801 tcccctgccc gtagacgata aatcgttgag cgaagtactg catcaggttg gtcttgggaa
     4861 attggctgcg cgtattcatg accacgatcg ctggggagat attctttcca gcggcgaaaa
     4921 acaacgtatc gccctggcac gattaatttt acgacgtccg aaatggatat ttcttgacga
     4981 aactacctct catcttgagg aacaagaggc tatccgctta ctgcgtttag tgcgtgaaaa
     5041 actacccaca agcggcgtca ttatggttac acatcaaccc ggtgtctgga acctggccga
     5101 tgatatttgt gacattagcg cggttttata aaaacaataa aagccctgct gtacggagat
     5161 ataaataaat gaagcgagtt cttattcctg gcgtcatttt atgtggcgct gatgtggcgc
     5221 aggccgtcga tgacaaaaac atgtacatgc atttttttga agagatgacg gtctatgctc
     5281 ctgtccctgt acccgtaaac ggcaacacgc attacaccag tgaaagcatc gagcgtttac
     5341 cgaccgggaa tggcaatatc agcgatctgc tgagaaccaa ccctgcggta cgcatggatt
     5401 caacgcaaag tacctcgttg aaccagggag atattcgccc tgagaaaatc tctattcacg
     5461 gtgcgtcgcc ctaccagaat gcctatttga ttgacggtat tagtgcaact aataacctga
     5521 acccagcgaa tgagtccgat gccagtagtg caaccaatat tagcgggatg tcacaggggt
     5581 attatcttga tgtcagctta ctggacaatg tgacgcttta tgacagtttt gtgccggttg
     5641 aatttggtcg cttcaatggc ggggtaattg atgcaaagat caaacgcttc aacgctgatg
     5701 atagcaaggt gaaattgggt tatcgcacta cgcgttcgga ctggttaaca tcgcatatcg
     5761 atgagaataa caagagcgca tttaatcaag gttcttcagg aagtacttat ttctctccag
     5821 attttaaaaa gaacttttat accttgtcgt ttaatcagga gctggctgat aactttggcg
     5881 ttaccgccgg tttatcgcgc cgccagtctg atatcacccg cgcggattat gtttcgaatg
     5941 acggcattgt cgtcggtcgg gcacagtata aaaacgttat cgatactgca ttgagcaaat
     6001 ttacctggtt tgccagcgac cgctttaccc acgatttaac cttaaaatat accggctcca
     6061 gccgtgatta taataccagc accttcccgc agtctgatcg cgaaatgggt aataaatcct
     6121 atggtcttgc atgggatatg gatacgcaac tcgcatgggc caaactacgt accaccgttg
     6181 gttgggatca tattagtgat tatacccgtc acgatcatga catctggtac accgaacttt
     6241 catgtacata tggtgatatt acagggcgtt gtacccgtgg cggattagga cacatttccc
     6301 aggctgtaga taattacacc ttcaaaacac gcctggactg gcaaaaattc gccgtgggtg
     6361 atgtttcgca tcaaccctac tccggcgcgg aatacatcta ttccgatgca tggactgaac
     6421 gccataacca gtctgaatcc tatgtgatta atgctgcagg aaagaaaact aaccatacca
     6481 tttaccataa aggtaaaggc agcctaggaa ttgacaacta cacgctgtat atggcggatc
     6541 acattagctg gcggaatgtg tcgttaatgc ccggtgtgcg ttatgactat gacaactatc
     6601 tgtcaaacca caatatctcc ccgcgcttta tgacggaatg ggatattttt gctgatcaaa
     6661 cctcaatgat taccgccggt tataaccgtt actatggcgg gaatattctt gatatgggat
     6721 tacgtgatat ccgcaatagc tggacggaat cggtatcagg taataaaacc ctgacgcgtt
     6781 atcagaattt gaaaacgcct tataacgatg aactggcaat gggattgcag caaaaaatcg
     6841 gtaagaacgt tattgcacgc gcaaactatg tttaccgtga agcgcatgat caaatcagca
     6901 aaagcagtcg taccgacagc gcgactaaaa ccaccattac tgaatataac aacgatggca
     6961 aaaccaaaac gcattcgttt aacctcagtt ttgaactggc cgaacccctg catatccgcc
     7021 aggtagatat taacccgcaa attgtcttta gctatatcaa gagcaagggc aacttgtcgt
     7081 taaacaatgg ttatgaggag agcaataccg gtgataacca ggtggtttat aacggtaatc
     7141 tggtctctta cgatagcgtt ccagtggcag attttaataa cccattaaag atctccttaa
     7201 acatggattt cacgcatcaa ccgagcgggt tagtgtgggc gaatacgctg gcgtggcaag
     7261 aagcgcgtaa agctcgcatt atccttggta agacaaatgc gcaatacatc agcgaatatt
     7321 cagattacaa gcagtatgtt gacgaaaaac tggatagcag cctgacctgg gacacccgct
     7381 tgtcctggac gccacaattt ctgaaacaac aaaacctgac gatcagtgcc gatattctca
     7441 atgtactgga tagcaaaacc gctgttgata caacgaatac cggtgtggcg acctacgcca
     7501 gtggccgtac tttctggctt gatgtcagca tgaaatttta atagttcgga ttttacttaa
     7561 ccgggtaaat tcgcccggtt ttcgcatgga gataattatg agaaacctct gtttcttact
     7621 gacgttagtg gcaactctgt tgctccccgg gcggctgatt gccgccgcct taccgcagga
     7681 tgaaaagtta attaccgggc aactggacaa tggcttgcga tatatgattt atccgcatgc
     7741 tcaaccaaag gatcaggtaa atttatggct gcaaattcat accggttcat tgcaggaaga
     7801 agacaatgag cgcggcgtgg ctcattttgt agaacatatg atgtttaacg gcacaaaaac
     7861 atggccgggt aataaagtca tcgaaacatt tgagtcaatg ggcctgcgtt ttggtcgcga
     7921 tgttaatgcc tataccagct atgacgaaac ggtgtatcag gtgagtttgc cgactacgca
     7981 gaaacaaaat ctgcaacaag tgatggcaat cttcagtgaa tggagtaatg ccgcaacctt
     8041 tgaaaaactc gaagtagacg ctgaacgtgg cgtaattact gaggaatggc gtgcccatca
     8101 ggatgcgaaa tggcgcacct ctcaggcgcg ccgccctttc ctgctggcta atacccgtaa
     8161 tttagaccgt gaacctatcg gcctgatgga tactgtcgcc acggtcacac cggcacaatt
     8221 gcgccaattt tatcaacgct ggtatcaacc aaataatatg acctttatcg tggtcggcga
     8281 tatcgacagt aaaaaagcgc tggcgctgat aaaggataat ttaagtaagc ttccggctaa
     8341 caaagcagct gaaaatcgcg tctggccgac aaaagccgaa aaccacctgc gctttaatat
     8401 cattaatgat aaagaaaacc gggtgaacgg catcgcactc tattatcgcc tgccaatggt
     8461 acaagtgaac gatgagcaaa gctttatcga acaagctgaa tggagcatgt tagttcagct
     8521 gttcaatcaa cgtctgcagg aacgcataca gtcgggcgag ttgaagacta tttctggcgg
     8581 cactgcgcgc agcgttaaaa ttgcacccga ttatcagtcg ctgtttttcc gtgtaaatgc
     8641 acgagacgat aatatgcagg atgctgcgaa tgcattaatg gcagagttgg caaccattga
     8701 tcagcatggt ttttctgctg aagaactcga tgatgtcaaa tctacccgcc tcacctggct
     8761 gaaaaatgcg gttgatcagc aagctgaacg tgatttacgt atgctgacca gtcgcctggc
     8821 atccagctca ttaaataata cgccgttctt gtcgccggaa gagacatatc aactttcgaa
     8881 acgtctgtgg cagcaaatta ccgtgcaaag tctggcggaa aaatggcagc agttaagaaa
     8941 gaaccaggac gcattttggg agcaaatggt aaacaatgag cttgccgcca aaaaagcatt
     9001 gtctcctgcg gctatcctgg cgctggaaaa agagtacgcc aacaaaaagc tggcggctta
     9061 catcttccca ggcagaaatt tatcgttaac agtagacgct gacccacagg cggaaattag
     9121 cagcaaagaa acgctggctg agaatctgac atcattaaca ctttccaatg gtgccagggt
     9181 tattctggca aaatccgcgg gtgaagagca aaagctacaa attactgccg tatcgaataa
     9241 aggcgattta agtttccctg cgcagcaaaa atcacttatc gcgctggcaa ataaagcagt
     9301 aagcggaagc ggcgttggtg agctctcctc ttccagcctg aaacgctgga gtgcggaaaa
     9361 ttcggtaacc atgagcagta aagtcagtgg catgaatacg ttgctctccg ttagcgcgcg
     9421 gactaataac cctgaacctg gttttcagtt gattaaccag cgaatcaccc acagcacgat
     9481 taacgacaat atttgggcat cgctacaaaa tgctcaaatt caggcgttga aaacgctcga
     9541 ccagcgtcca gcggagaaat tcgcccagca gatgtatgag acgcgctatg ctgatgaccg
     9601 cacgaaatta ctgcaagaaa atcagattgt acagtttact gccgcagatg cgctggctgc
     9661 cgatcgccag ttgttttcat ctccagcgga tatcacgttt gtcattgtcg gtaatgtctc
     9721 agaagacaaa ctcgtggcgt taattacgcg ttacttagga tcaatcaaac actctgattc
     9781 gccattagcc gcaggtaaac cattaactcg cgcgacggac aacgcatcgg ttactgtaaa
     9841 agaacaaaat gaacctgtgg cacaggtttc acagtggaag cgttatgatt cccggacacc
     9901 tgttaatctg gcgacgcgta tggcgctcga tgcttttaac gtcgcactgg caaaagatct
     9961 acgtgttaat attcgtgaac aggcatctgg agcatacagc gtttcttctc gcctctcggt
    10021 tgatcctcag gccaaagata tcagtcattt gctggctttt acttgtcaac cagaacgaca
    10081 tgatgaactg ttaacgttag cgaatgaagt gatggttaag cgtctggcta aagggatcag
    10141 tgagcaagaa ctgaatgaat accagcaaaa cgttcagcgc agcctcgata tccaacagcg
    10201 tagcgttcaa caattagcga acactattgt aaatagtctt attcaatatg acgatcctgc
    10261 agcatggact gagcaggagc aattgttgaa acaaatgacg gtagagaatg ttaacactgc
    10321 cgttaaacaa tatctttctc atccggtaaa tacttatacc ggagtattat tgccaaaata
    10381 ataacagccc cgtcaacaca tcgttggcgg ggattttagc aatattcgct atttttatgt
    10441 aataatttta taaatgcg
//
LOCUS       DAZPEB010000145        10351 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZPEB010000145 DAZPEB010000000
VERSION     DAZPEB010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10351
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.148"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            1..900
                     /locus_tag="ACHURJ_003126"
                     /pseudo
     CDS             1..900
                     /locus_tag="ACHURJ_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
     gene            complement(1010..2053)
                     /locus_tag="ACHURJ_003127"
     CDS             complement(1010..2053)
                     /locus_tag="ACHURJ_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFR1281974.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPEASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKNPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(2179..2754)
                     /gene="nudK"
                     /locus_tag="ACHURJ_003128"
     CDS             complement(2179..2754)
                     /gene="nudK"
                     /locus_tag="ACHURJ_003128"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFR1281975.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLTRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            complement(2822..4801)
                     /gene="aegA"
                     /locus_tag="ACHURJ_003129"
     CDS             complement(2822..4801)
                     /gene="aegA"
                     /locus_tag="ACHURJ_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFR1281976.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHTSTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEMKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            5007..6707
                     /gene="narQ"
                     /locus_tag="ACHURJ_003130"
     CDS             5007..6707
                     /gene="narQ"
                     /locus_tag="ACHURJ_003130"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFR1281977.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQHLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            6870..9978
                     /gene="acrD"
                     /locus_tag="ACHURJ_003131"
                     /pseudo
     CDS             6870..9978
                     /gene="acrD"
                     /locus_tag="ACHURJ_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
     gene            complement(10077..10136)
                     /gene="ypfM"
                     /locus_tag="ACHURJ_003132"
     CDS             complement(10077..10136)
                     /gene="ypfM"
                     /locus_tag="ACHURJ_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFR1281978.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT         2360 a         2736 c         2744 g         2511 t
ORIGIN      
        1 gccgatgctg cctgagttgc tcggcggttc ggcggatctg gctccagcaa cctgaccatc
       61 tggaaaggtt ctgtttcgct gaaggaagat ccggcgggca actacattca ctacggggtg
      121 cgtgaatttg gcatgaccgc tatagccaac ggcatcgcgc accacggcgg ctttgtgccg
      181 tataccgcca cgttcctgat gtttgttgaa tacgcccgta acgccgcgcg gatggcggca
      241 ctgatgaaag cgcggcagat tatggtttat acccacgact caattggcct gggcgaagat
      301 ggtccgacgc accaggctgt tgagcaactg gccagcctgc gcttaacgcc aaatttcagc
      361 acctggcgac cgtgcgatca ggtggaagcg gcggtgggct ggaagctggc ggttgagcgc
      421 cacaacggac cgacggcact gatcctctca aggcagaatc tggcccaggt ggaacgtacg
      481 ccggatcagg taaaagagat tgcccgtggc ggttatgtgc tgaaagacag cggcggtaag
      541 ccagatatta ttttgattgc caccggttca gagatggaaa tccccctgca agcggcagag
      601 aaattagcgg gagaaggtcg caatgtacgc gtagtttccc tgccctcgac tgatattttc
      661 gacgcccagg atgaggaata tcgggagtcg gtgttgcctt ctaacgttgc ggctcgcgtg
      721 gcggtggaag caggtattgc cgattactgg tacaagtatg ttggtttgaa aggggcgatt
      781 gttgggatga caggttatgg ggaatctgct ccggcggata agctgttccc gttctttggc
      841 tttaccgccg agaatattgt ggcaaaagcg cataaggtgc tgggagtgaa aggtgcctga
      901 tggtgatcgc cggatgctga ttgccggacg ctgattgccg gatgcgactc tgacgcgtct
      961 tatccggcct acagcgtctt atccggccta catgtccccg ccattttgtt taacgggtga
     1021 tccacaacgt gggccaggcg tctggcccat gccagttatc gcaggtgggt tctgcggcgt
     1081 aacgcaccag gcgaaaacgc tgaccgtcaa agcgccagcg cgcctgaatg ccacaatcgc
     1141 ttaatccgcg ccctttcgct aaggtcacca gttcacgcga tttctcatca aatgttgcgt
     1201 tcatcagttc cagttcattc gtctcctgcc cgttgttgaa cggcaaacgc aaccgaaccg
     1261 ggcgcgaggc cagcggattt ttacgcgaca caatccatgc caaatcaatg gtgttatagg
     1321 cccctgcctc acagctaatc atcatcagcg ctttatcatc agtcagcgca gtgacattca
     1381 cctcacgacg caatggatca agcgagcagc gcagaccatt catccgccag tttccataat
     1441 ccagcaaatc gttgcgttct tcgagtgaga gtggcgtcgg cgttgggtta accaccgcga
     1501 cctctttcag cgcaggcgcg ggcggtacgc tgagcggcgg ttcgtcccct ttcttgatcc
     1561 acgcggtttc actgccaacg cgcttttgct gagcatcaat aaacaacaac gccgctttta
     1621 agccactcag cgaaatggtc tgattgccgt cgcgtaaggt gatcgccttc ccttcctgaa
     1681 tcatctgcaa aaacgcggtg atggttgccg tatcatcggt tactaataac catggtgaaa
     1741 tccgccactt gtcaccactt aacgccaacg gctcgccatc taacagcagc cgtggcgcta
     1801 tctccccttc tgacgcctcc ggcgacttca atccgccgcg ctcaatacgt aaaacggcat
     1861 cggtatgcgc cccggcgctg cggctcaggg tcatcaccag tccattatga tcgcccgtat
     1921 tacgcgccac gcagaagttt tggttattac aggtgacctg ccagtcggaa aacgcccgtt
     1981 gcgctggtgc cgcccacacc aaagacgtcg gcaacaaagc gaaaaaaaag agaaggaaaa
     2041 tgcgatagcg catggacggt acgaccccag aagttcaaac aaacagcaca agtcgtatct
     2101 tcgttgcgca ggtagcgcgg ctcaatcgga tttgtcggat tgagccaata ataaacaacg
     2161 aaatattatt gttatttttc agtccattaa atgtgacgtt tgcaaatagt taagcaataa
     2221 caccgtctta ccgtcacgta tctcgccggt tttgatcatc tccagcgcct ggctgaacgg
     2281 cagctcgagc acttcaatat cttcatcttc gacaccgcca ccggcgttag cgcgctgatt
     2341 gtcactgtat tcggcgataa aaaagtggat tagctcagtc acaccgcctg gcgacatata
     2401 cagttcaaat aatttgcgca cttcgcccac ttcatagccc gtctcttcaa tcgcttcttt
     2461 acgaatgcac acttccggtt cgtcgttatc cagcagcccg gcgcaggttt caatcagctg
     2521 cccgctttca ttgccattaa cccaggtagc gacacggaac tgacgagtca gaaccacggt
     2581 ctttttcttc gcgttgtaca ggaggatcgt cgcaccatta ccgcgatcgt aaacttcacg
     2641 tttatggcgg ataacttcgc catctttgcg cgtgagatcg taggtaatgt tgtgcagggt
     2701 gaaatagtta tcggagagaa ttttgtcttt aatgagggtg atttgttgcg tcataccgac
     2761 tccacagcgc gaaatgaaca attatcttac gctgtgaagt cgggtttgtc tgcgcaggct
     2821 atcagtgaga tttgactgat tttaccccca gccagtcaat aatcccctgt gccgcatgac
     2881 gtccttctgc cattgcggta accaccagat ccgcaccacg cacggcgtcg ccaccggcga
     2941 agattttcgg attggtggtc tggtaacggt actggctttc cacatccgcg atgatgcggc
     3001 cccatttgtc taccgttaca ccgtgcgact ccagccacgg catcccgtgc ggattgaagc
     3061 caaacgccat aatcaccgcg tcggctggca tgacaaattc actgccttcc accggtactg
     3121 gacgccgacg cccctgggca tccggctcgc caagacgcgt gcgcaggaaa cgaatcccgc
     3181 aaacgtgacc ttgttcattc agctcaagcg ccaccggctg gacgttaaat tcgaagttgg
     3241 ccccctcttc gcgggcgttc ttcatctctt tcttcgagcc tggcatgtta gcttcatcac
     3301 gacgataagc gcaggtgacg ttactcgcgc cgtggcgcag cgcagtacgc acacagtcca
     3361 tcgcggtgtc gccgccgccc agtaccacga cgttaagtcc ggcggtattg ataaacggct
     3421 cttccggtag ctcttcgagc cccatcacct gtttagtgtt ggcgatgagg aacggcagcg
     3481 cgtcataaac gcccggcgca tcttcattgg gtaaacccgc tttcatggaa cggtaagtgc
     3541 ctacgccaac gaagaccgcg tcgtattgtt ccaaaagcga atccaaagag acatctttac
     3601 ccacttcaca attgagttcg aagtgaatcc ccatcgcgct gaagatttcc cgacggcgtg
     3661 ccagcaggga tttatccagt ttgaaagaag gaatgccgaa agtgagcaag ccaccgattt
     3721 ctggatggcg atcgtacacc gtcaccgcca cgccattgcg ggtcagaaca tccgcacagg
     3781 ccagccctgc cggacctgcc ccgataatcg ccacccgctt gtccactttg gtgacatgac
     3841 ttaagtcagg acgccaacct ttcgccaacg cctgatctga aatatagcgt tcaatgttgc
     3901 cgatagttac cgcgccgtgc tcatcgcgaa tagtacaggc accttcacac aaacggtctt
     3961 gcggacaaac gcgtccggta atttccggca gggtgttggt ctggtgagaa agctcgacgg
     4021 cggcgtcgat gtttccggct ttcaccagtt caatccactg cggaatatgg ttatgcagcg
     4081 ggcaggtcca ttcacaaacg ctgtgctcgc cgcacttaag gcagcgcgag gcttcccgtt
     4141 gtgcctggtc ggcgcgaaat ggcagataaa tttcatcaaa accggttttg cgcgcttcaa
     4201 tcgccagttt atccggctcg ccacgcgcgg gcgttgcctg catttgttcg actttactca
     4261 ttaccggcat ttcttgtgca gcggtactgg tatgccacgg ttgatgctcc tgacgtgcgg
     4321 tgcgcaagcg gcgggatttc gccatgccgg agagtgcgac gtcagtgacc agttgcagcg
     4381 cgtccgccgg gcaattctcg acacaggcag gaccgttttc gcgccctgca caaaggtcgc
     4441 atttatgcgc cgtggctttt acttttcctg ccgcgacggg tgtcaggacg atttgcatcg
     4501 taccaaacgg acaggccacc acgcaggatt tacagccaat acacttttgc tgattgacct
     4561 gaatgctgtc atcaacgtgg ctgattgcgc cattagggca gctacgggcg cagggcgcat
     4621 cttcacaatg gtgacaggtc actgcactac gttgctgctg atgtttgata accgtaattc
     4681 ggggatgaaa atggtgttgg ctcaggacat gttgctcatc attgtgagcc atgacacagg
     4741 cgatttcaca agcatgacaa cccagacatt gctgactgtt ggccataata aaacgattca
     4801 taacgacctt cttttttggt tgtaaaaacc ttattcttta tatgagtgtt gttattaccc
     4861 gacttacagg ggaatcggca atgttcatgt gcccagaata agtaactatt tcgctataaa
     4921 ctgtggcagc tcaaataatc ccatcactga ctaaattgcg tttcagcgaa ctggaacatt
     4981 aatgattttt tgtggagaag acgcgtgtga ttgttaaacg acccgtctcg gccagtctgg
     5041 cccgggcctt tttttacatt gtgctgctgt cgattctttc cacgggtatc gctctgctaa
     5101 ctctggcgag cagtttgcgc gacgctgagg ctatcaatat tgccggatcg ctgcgtatgc
     5161 agagttaccg cctgggctac gacttgcaaa gtggcagtcc acaactcaat gcacatcgcc
     5221 agttgtttca gcaggcactg cattcaccgg tattaaccaa tctcaacgtc tggtatgtgc
     5281 cagaagcagt aaaaactcgc tatgcgcatc tgaatgccaa ctggctggag atgaataatc
     5341 ggctcagcaa gggcgatttg ccgtggtatc aggccaatat taataattat gttaatcaga
     5401 tagacctgtt cgtgctggct ttacagcact acgctgaacg caaaatgctg ctggtggtgg
     5461 cgatttccct ggctggcggt atcggtattt tcacgctggt cttttttact ttgcgccgta
     5521 tacgccatca ggtggttgcc ccgctgaatc agctggttac cgccagtcaa cgtattgaac
     5581 acgggcagtt cgactcgccg ccgctggata ccagtctgcc gaatgagctt ggtctgcttg
     5641 caaaaacctt taaccagatg tcgagcgagc tgcataaatt gtacctttcg ctggaggcgt
     5701 cagtagaaga aaagacccgc gatctccacg aggccaagcg tcgtctggag gtgttgtatc
     5761 aatgttcgca ggcgctgaac acaagccaga ttgatgtgca ttgtttccgc catattttgc
     5821 agattgttcg cgacaatgaa gcggctgaat atctggagtt aaatgtcggt gaaaactggc
     5881 ggattagcga agggcaacca aacccggaat tgccgatgca gattttaccg gtgacaatgc
     5941 aagagacggt ttacggcgaa ctgcactggc aaaatagtca cgtttcatca tcagaaccgc
     6001 tgcttaacag cgtttcgtcg atgctgggac gcggtttgta ctttaatcag gcgcagaagc
     6061 attttcagca tttattgttg atggaagaac gtgcaaccat cgcccgcgaa ttgcacgact
     6121 cgctggctca ggtactttct tacttacgta tccagttgac gttactgaag cgttcgatac
     6181 cggaagataa cgccaccgca caaagtatca tggccgattt ttcccaggcg ttgaatgatg
     6241 cttatcggca gttacgcgag ctgttgacta ctttccgcct gacgctgcag caggcggatc
     6301 tcccctccgc gttgagggaa atgctggata cgttacaaaa tcaaaccagc gccaaactga
     6361 ccctcgactg ccgtctgcca acccttgcgc tggatgcgca aatgcaggtg catttgttgc
     6421 aaattattcg cgaagcggtg ctgaatgcga tgaagcacgc caacgccagc gaaatcgccg
     6481 tcagttgcgt caccgcgccg gacggcaatc acacggttta tatccgtgat aacgggattg
     6541 gtatcggtga accgaaagaa cccgaaggtc attatggtct gaatatcatg cgcgaacgcg
     6601 cggaacggct gggtgggacg ctgacttttt cacaaccttc cggcggcggc acgttagtga
     6661 gtattagctt tcgctctgca gagggtgagg aaagtcagtt gatgtaatgc ctcctactga
     6721 ccaaagaata cttgcactta aggttcagta taaaagggca tgataattta cattaactcc
     6781 ttttttctcc acgattggct cgtaccttgc cgctacagtg aagcaagtca agcctacaac
     6841 gatacgcaga aacacgaggt cctcttttaa tggcgaattt ctttattgat cgccccattt
     6901 ttgcctgggt gctggcaatc ctgttgtgtc tgacagggac cctagcgatt ttttcattgc
     6961 ccgttgaaca ataccccgat ctcgcgccac cgaatgtgcg agtgaccgct aactatcccg
     7021 gcgcatcggc ccagacgctg gaaaacaccg tgccccaggt tatcgagcaa aatatgaccg
     7081 gcctcgataa tctcatgtat atgtcatctc agagcagtgg caccggtcag gcatctgtca
     7141 ctttaagttt taaagcaggc accgatccgg acgaagccgt gcagcaagta caaaaccagc
     7201 tgcaatcagc catgcgaaag ttaccgcagg cggtgcaaaa tcagggcgtg acggtgcgta
     7261 aaaccggcga taccaatatt ctgaccattg ctttcgtctc taccgatggt tcgatggata
     7321 aacaggatat tgccgattat gttgccagta atattcagga cccgttaagc cgcgtgaatg
     7381 gcgtcgggga tatcgatgcc tatggttcgc aatattccat gcgtatctgg ctggccccgg
     7441 cgaaactcaa cagtttccag atgacggcta aagatgtcac tgatgccatt gagtcacaga
     7501 acgcgcagat tgcggttggg caacttggtg gtacaccttc cgtcgataag caggcgctca
     7561 acgccaccat taacgcccag tcactgctgc aaacaccaga acagttccgc gatatcacct
     7621 tgcgggtgaa tcaagacggc tcagaggtaa ggctgggcga tgtcgccacc gtcgaaatgg
     7681 gggcggagaa atacgattat cttagccgct tcaatggtaa gccagcctcc gggctggggg
     7741 taaaactggc ctccggcgca aacgaaatgg cgacagggag ctggtgctca atcgtctcga
     7801 cgagctggcg cagtacttcc cgcacggact ggaatacaag gtggcgtatg aaacaacctc
     7861 gtttgttaaa gcctccattg aagacgtggt gaaaacgctg ctggaagcta tcgccctggt
     7921 tttcctcgtt atgtatctgt tcctgcaaaa cttccgcgcc acgctgatac ccactatcgc
     7981 cgtgccggtg gtgttgatgg gaaccttctc cgtactttac gccttcggtt acagcgtcaa
     8041 caccttaacc atgttcgcga tggtactggc gatcggtctg ctggtggatg acgcaatcgt
     8101 ggtggtggaa aacgtcgaac gtattatgag tgaggaagga ctcactcctc gcgaagccac
     8161 acgtaaatcg atggggcaga tccagggggc actggtcggg attgcgatgg tgctttcggc
     8221 ggtatttgta ccaatggcct tcttcggcgg caccaccggt gccatctatc gccagttctc
     8281 tattaccatt gttgcggcga tggtgctgtc agtactggta gcgatgatcc tcactccggc
     8341 tctgtgtgcc acactactta agccactgaa aaaaggtgag catcacgggc aaaaaggctt
     8401 ttttgcctgg tttaaccaga tgtttaaccg caacgccgaa cgctacgaaa aaggggtggc
     8461 gaaaattctc caccgcagcc tgcgctggat tgtgatttat gtcctgctgc ttggcggcat
     8521 ggtgttcctg ttcctgcgtt tgccgacgtc gttcttaccg ctggaagacc gtggcatgtt
     8581 tactacctcg gtacaattgc ccagcggttc aacccaacaa cagaccctga aagtcgttga
     8641 gcaaatcgag aaatattact tcacccatga aaaagacaac atcatgtcgg tgtttgccac
     8701 cgttggttct ggccctgggg gtaacgggca aaacgtggcg cgaatgttta tccgcctgaa
     8761 agactggagc gaacgcgaca gtaagaccgg cacctcgttt gccattatcg agcgtgcaac
     8821 aaaggcgttt aacaagatta aagaagctcg cgttatcgcc agcagcccgc cagcaattag
     8881 cggtcttggt agttctgcgg gttttgatat ggagttgcag gaccacgctg gagcaggtca
     8941 cgatgcgctg atggcagcac gtaaccagtt gctggcgctg gcggcggaaa acccggagct
     9001 aacccgtgtg cgccataacg gcctcgatga cagtccgcag ttgcagattg atatcgacca
     9061 gcgtaaagct caggcgctgg gcgttgctat cgacgatatt aacgacacac tgcaaaccgc
     9121 ctggggttcg agctatgtga atgactttat ggatcgcggt cgtgtgaaga aagtctatgt
     9181 gcaggcagct gcgccgtatc gcatgctgcc agatgacatt aatctctggt atgtccgaaa
     9241 taaagatggc ggcatggtgc ccttctctgc tttcgcgacc tcacgctggg agactggctc
     9301 gccgcgtctg gaacgctata acggttattc tgcggttgag attgttgggg aagccgcacc
     9361 gggggtcagt accggtacgg cgatggatat aatggaatcg ttagtgaagc agttgccaaa
     9421 cggctttggt ctggagtgga cggcgatgtc gtatcaggag cggctttccg gcgcgcaggc
     9481 tccggcgctg tacgccattt ccttgctggt ggtattcctg tgtctggcgg cgttgaaagc
     9541 tggtcggtgc cgttctcggt aatgctggtc gtgccgctgg gggttatcgg cgcgctgctg
     9601 gcaacctgga tgcgcgggct ggaaaacgac gtttacttcc aggtgggcct gttaacggtc
     9661 attggtttat cggcgaaaaa cgccatcctg atcgtcgagt ttgctaacga gatgaaccaa
     9721 aaaggccacg acctgtttga agcgacgctc cacgcctgcc gtcagcgttt acgcccgatt
     9781 ctgatgacct cgctggcatt tatcttcggc gtattgccaa tggcaaccag cacgggtgcc
     9841 ggttccggtg gtcagcatgc ggtgggtact ggcgtaatgg gcgggatgat ttcggccact
     9901 attctggcta tttacttcgt gccgctgttc tttgtactgg tgcgccgccg cttcccgctg
     9961 aagccgcgcc cggaataagc aataaaaaag gcgacatgct aatgtgtcgc ctttttcaac
    10021 tttccgatta agaacctgct cagcgggttc ttgctgtttg tactttgtct caggaattac
    10081 ttacgaagca taacttcgat aaagtctttc cagttcccca gttcacgttc aatcataaca
    10141 acctctctta taattatggg tattctacgg aaacaatata ccgtggtgaa gctaatttac
    10201 tcgattgctg cgatgactac ctccggggga caaaccttat gtaaatacta tggtcctgcg
    10261 gtgataattt gtatgtgata cacagcaaca tttcgagata ttcatacggc atctaatact
    10321 gatttaattc tggttaaaat acagacagat a
//
LOCUS       DAZPEB010000146        10332 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZPEB010000146 DAZPEB010000000
VERSION     DAZPEB010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10332
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.149"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            121..987
                     /gene="folD"
                     /locus_tag="ACHURJ_003133"
     CDS             121..987
                     /gene="folD"
                     /locus_tag="ACHURJ_003133"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFR1281979.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            989..1201
                     /gene="ybcJ"
                     /locus_tag="ACHURJ_003134"
     CDS             989..1201
                     /gene="ybcJ"
                     /locus_tag="ACHURJ_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFR1281980.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1309..1830
                     /locus_tag="ACHURJ_003135"
     CDS             1309..1830
                     /locus_tag="ACHURJ_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR1281981.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMVLMGMLWWRRR"
     gene            complement(1866..3251)
                     /gene="cysS"
                     /locus_tag="ACHURJ_003136"
     CDS             complement(1866..3251)
                     /gene="cysS"
                     /locus_tag="ACHURJ_003136"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFR1281982.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQTRAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3425..3919
                     /gene="ppiB"
                     /locus_tag="ACHURJ_003137"
     CDS             3425..3919
                     /gene="ppiB"
                     /locus_tag="ACHURJ_003137"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFR1281983.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            3922..4644
                     /gene="lpxH"
                     /locus_tag="ACHURJ_003138"
     CDS             3922..4644
                     /gene="lpxH"
                     /locus_tag="ACHURJ_003138"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFR1281984.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIVARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4762..5271
                     /gene="purE"
                     /locus_tag="ACHURJ_003139"
     CDS             4762..5271
                     /gene="purE"
                     /locus_tag="ACHURJ_003139"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFR1281985.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            5268..6335
                     /gene="purK"
                     /locus_tag="ACHURJ_003140"
     CDS             5268..6335
                     /gene="purK"
                     /locus_tag="ACHURJ_003140"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFR1281986.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETVLTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YMGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDANYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(6472..7363)
                     /locus_tag="ACHURJ_003141"
                     /pseudo
     CDS             complement(6472..7363)
                     /locus_tag="ACHURJ_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
     gene            complement(7360..8175)
                     /locus_tag="ACHURJ_003142"
     CDS             complement(7360..8175)
                     /locus_tag="ACHURJ_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFR1281987.1"
                     /translation="MTIIHPLLASSSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     SHPQPLAAAWMQRAEETGLFGPLAMAASDPLTAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARAGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8186..9445)
                     /locus_tag="ACHURJ_003143"
     CDS             complement(8186..9445)
                     /locus_tag="ACHURJ_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFR1281988.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPAFSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGGCLFEGWAKDEAQALAMLEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMEHGIDLTAMMAQGITMGDEFHQRNIASSALLMRTLAPQIARLDHDKQHIAEV
                     IDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(9455..10332)
                     /gene="fdrA"
                     /locus_tag="ACHURJ_003144"
                     /pseudo
     CDS             complement(9455..10332)
                     /gene="fdrA"
                     /locus_tag="ACHURJ_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415051.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
BASE COUNT         2271 a         2936 c         2777 g         2348 t
ORIGIN      
        1 aaccttcgca agaggtcgct tcacgcgata aatctgaaac gaaacctgac agcgcgcccc
       61 gcttctgaca aaataggcgc atccccttcg acctacgtaa cagatggaat cctctctctg
      121 atggcagcaa agattattga cggtaaaacg attgcgcagc aggtgcgctc tgaagttgct
      181 caaaaagttc aggcgcgtat tgcagccgga ctgcgggcac caggactggc cgttgtgctg
      241 gtgggtagta accctgcatc gcaaatttat gtcgcaagca aacgcaaggc ttgtgaagaa
      301 gtcgggttcg tctcccgctc ttatgacctc ccggaaacca ccagcgaagc ggagctgttg
      361 gagcttatcg atgcgctgaa tgccgacaac accatcgatg gcattctggt tcaactgccg
      421 ttaccggcgg gtattgataa cgtcaaagtg ctggaacgta ttcatccgga caaagacgtg
      481 gacggtttcc atccttataa cgtcggtcgt ctgtgccagc gcgcgccgcg tctgcgtccc
      541 tgcaccccgc gcggtatcgt cacgctgctt gagcgttaca acattgatac cttcggcctt
      601 aacgccgtgg tgattggcgc atcgaatatc gtcggtcgtc caatgagcat ggaactgttg
      661 ctcgcaggtt gcaccactac cgtgactcac cgtttcacta aaaatctgcg tcaccacgta
      721 gaaaatgccg atctgttgat cgttgccgtc ggcaagccgg gctttattcc tggtgactgg
      781 atcaaagaag gcgcaattgt gattgatgtc ggcatcaacc gtctggaaaa tggcaaagtt
      841 gtgggcgacg tcgtgtttga agacgcggct aaacgcgcct catacattac gcctgttccg
      901 ggcggcgttg gcccgatgac ggttgccacg ctgattgaaa acacgcttca ggcgtgcgtt
      961 gaatatcatg atccacaggg tgagtaacat ggcgacattt tctttaggta aacatccgca
     1021 cgttgagctg tgcgatttgc tgaaactgga aggctggagc gaaagcggcg cgcaggcgaa
     1081 aatcgcgatt gccgaaggcc aggtgaaagt cgacggtgcg gttgaaacgc gcaaacgctg
     1141 caaaatcgtc gccggtcaga cagtgagttt tgcaggtcac agcgtacagg ttgttgcctg
     1201 atccctttcc cggcctgtat caacaggccg gttctctttt cagacttttg ccctctacgc
     1261 cccgttcatc catgctgata gcatcgacat tttgccgaag cgaccatcat gccaaccgtt
     1321 attacgcacg ccgccgttcc cctatgcatt ggtttagggt tgggttcgaa agtcatccct
     1381 ccgcgtctgt tatttgccgg aatcatcctg gcgatgctgc ccgacgccga cgtattgtcg
     1441 tttaaatttg gcgttgctta cggcaatgtt tttggtcatc gcgggtttac ccattcgctg
     1501 gtgtttgcgt ttgttgtccc gctgttatgt gtgctgattg gacgccgatg gttcagggct
     1561 gggcttattc gctgctggct gtttttaacc gtctcgttgc tatcgcacag cttgctggat
     1621 tcggtaacca ctggcggtaa gggcgttggc tggctgtggc cgtggtcaga tgaacgcttt
     1681 ttcgctccct ggcaggtgat taaagtcgcg ccgtttgcat tgtctcgtta caccacgccg
     1741 tacgggcatc aggtgataat ttctgaattg atgtgggtgt ggttgccggg gatggtgctt
     1801 atgggaatgt tgtggtggcg caggcgataa ccggatgcga aaactcgcat ccggcaacag
     1861 cgcaattact tacgacgcca ggtggtccct tgcgggccat cttccagcac gatccccatc
     1921 tcgttaagac ggtcacgcgc cgcatctgcc gccgcccagt ctttcgcttt acgggcatcc
     1981 agacgctgtt gaattaacgc ttcaatctca gccacttcgc tgtcgtctgc ctgcgcgccg
     2041 ctttgcagga acgcttccgg ttcttgctcc agcaggccca atacagcgga aagtttacgc
     2101 aggtgagatg ccattgcatt cgccgctgcc atatcttctg ctttcagacg gtttacttca
     2161 cgcgccatat caaacagtac ggaataggct tccggggtgt tgaaatcgtc gtccatcgct
     2221 tcaataaagc gcgcttcaaa cgcttcgcca ccggcaggcg caacggtttt atctgtgccg
     2281 cgcagcgcag tgtagagacg ctccagcgcc gcacgcgtct gcttcaggtt ctcttcgctg
     2341 tagttcaact ggctgcgata gtggcccgac atcaggaagt aacgcacggt ttccgcgtcg
     2401 tagtatttca gcacatcgcg cacggtaaag aagttaccca gcgatttgga catcttctcg
     2461 cggtcaacca tcaccattcc ggagtgcatc cagtagttca catactgacc atcatgggca
     2521 caggtggact gcgcgatttc gttctcgtgg tgcgggaaca tcaggtctga accgccgccg
     2581 tggatatcaa agtggttacc cagctgcttg cagttcatgg ccgaacattc aatgtgccag
     2641 ccaggacgac ccgcgcccca cggagacggc cagctcggtt cgccctcttt cgacatcttc
     2701 cacagaacga agtccatcgg gttgcgtttg tcatcaacca cgtcaacgcg cgcgccagcc
     2761 tgcagctggt cgagatcctg acgcgacagc acgccataag tcggatcggt cgggacgtcg
     2821 aacatcacgt cgccgttgtc agccacataa gcgtgacctt tggcgatcag ttgttcagtg
     2881 agttcaataa tttctgcgat atggtgcgtc gcgcgcggct ccatatccgg gcgcagaatg
     2941 ttcaaagcat caaaatcttt gtgcatttcg gcgatcatgc gatccaccag cgccacaaag
     3001 ctttcgccat tttcattggc gcgtttgatg attttgtcgt cgatatcggt aatgttgcgc
     3061 acatacttca gcttatagcc gaggaaacgc agatagcgcg caaccacgtc aaaagcaaca
     3121 aaggtacgcc cgtgaccgat atgacagaga tcgtaaacgg tgattccaca cacgtacatg
     3181 ccgacttccc cggcgtgaat aggcttaaat tcctcttttt ggcgtgtcag agtattgaag
     3241 atttttagca tcgaagattc cgtttagaca tgtgtgggta attgagttgc gtataatacc
     3301 catatttccc gcgcgaatca gcatacattg cgtgatgatc cgatcgtgcg gttatgctat
     3361 aacaccaccc tatatatgac ccgaaccggg ttgaagcacc aatcaaacgg aacaggatgc
     3421 aaaaatggtt actttccaca ccaatcacgg cgatattgtc atcaaaactt ttgacgataa
     3481 agcacctgaa acagttaaaa acttcctgga ctactgccgc gaaggttttt acaacaacac
     3541 cattttccac cgtgttatca acggctttat gattcagggc ggcggttttg aaccgggcat
     3601 gaaacaaaaa gccaccaaag aaccgatcaa aaacgaagcc aacaacggtc tgaaaaatac
     3661 ccgtggtacg ctggcaatgg cccgtactca ggctccgcac tctgcaactg cacagttctt
     3721 catcaacgtg gttgataacg acttcctgaa cttctctggc gaaagcctgc aaggttgggg
     3781 ctactgcgtg tttgctgaag tggttgaagg catggacgtg gtagacaaaa tcaaaggtgt
     3841 tgcaaccggt cgtagcggta tgcaccagga cgtgccaaaa gaagacgtta tcattgaaag
     3901 cgtgaccgtt agcgagtaat cgtggcgaca ctctttattg cagatcttca tctctgcgtg
     3961 gaagaaccgg cgatcaccgc cggttttctg cgttttttag cgggggaagc tcgcaaggcc
     4021 gacgcgctgt atattcttgg cgatctgttt gaagcgtgga ttggcgacga cgatcccaat
     4081 ccactccatc gccagatggc ggcagcgatc aaagcggtgt ccgattccgg cgttccctgt
     4141 tatttcattc atggcaaccg tgattttctg ctcggcaaac gctttgcccg tgaaagtggc
     4201 atgacgttat tgccggaaga aaaggtgctc gaactttatg gccgccgggt gttgattatg
     4261 catggcgaca cgctgtgcac cgatgacgcg ggttatcagg cttttcgcgc caaagtccac
     4321 aaaccctggc tgcaaatgct attcctcgcc ctgccgttgt ttgtgcgcaa acgcattgtc
     4381 gcgcgaatgc gcgcgaacag caaagaagcc aacagcagta aatcgctggc gatcatggac
     4441 gttaaccaaa acgcagtggt cagtgcgatg gaaaaacatc aggtgcaatg gctgatccac
     4501 gggcataccc atcgcccggc ggtgcatgaa cttatcgcca accagcaacc tgcttttcgc
     4561 gtggtactgg gtgcctggca tacggaaggt tcgatggtga aagtcacggc ggatgacgtt
     4621 gagctgattc attttccgtt ttaaaaaaac cacaactttg ctgatttcac agccacgcaa
     4681 ccgttttcct tgctctcttt ccgtgctatt ctctgtgccc tctaaagccg agagttgtgc
     4741 accacaggag ttttaagacg catgtcttcc cgcaataatc cggcgcgtgt cgccatcgtg
     4801 atggggtcca aaagcgactg ggctaccatg cagttcgccg ccgaaatctt cgaaatcctg
     4861 aatgtcccgc accacgttga agtggtttct gctcaccgca cccccgataa actgttcagc
     4921 ttcgccgaaa gcgccgaaga gaacggttat caggtgatta ttgcgggcgc aggtggcgca
     4981 gcgcacctgc caggcatgat tgccgccaaa acgctggtgc cggtgctggg cgtgccagta
     5041 cagagcgccg cactgagcgg tgtcgatagc ctctactcca tcgtacaaat gccgcgcggc
     5101 attccggtgg gtacgctggc gattggtaaa gctggcgcgg caaacgcggc attactggca
     5161 gcacaaattc tcgcgactca cgataaagag ctacaccagc gtctgaatga ctggcgcaaa
     5221 gcccagaccg acgaagtgct ggaaaatccg gacccgcgag gtgcggcatg aaacaggttt
     5281 gcgtcctcgg taacgggcag ttaggccgta tgctgcgtca ggcaggcgaa ccgttaggca
     5341 ttgctgtctg gccggtcggg ctggacgctg aaccggcggc ggtgcctttt caacaaagcg
     5401 tgattaccgc tgagatcgaa cgctggccgg aaaccgtatt aacccgcgag ctggcgcgtc
     5461 atccggcctt tgtgaaccgc gatgtgttcc cgattattgc tgaccgtctg actcagaagc
     5521 agcttttcga taagctccac ctgccgaccg cgccgtggca gttacttgcc gatcgcagcg
     5581 agtggcctgc ggtgtttgat cgtttaggtg aactggcgat tgttaagcgt cgcactggtg
     5641 gctatgacgg tcgcggtcaa tggcgtttac gcgccaatga aaccgaacag ttaccggcag
     5701 agtgttacgg cgaatgtatt gtcgagcagg gcattaactt ctctggtgaa gtgtcgctgg
     5761 ttggcgcgcg cggctttgat ggcagcaccg tgttttatcc gctgacgcat aacctgcatc
     5821 aggacggtat tttgcgcacc agcgtcgctt ttccgcaggc caacgcacag cagcaggcgc
     5881 aagccgaaga gatgctgtcg gcgattatgc aggagctggg ctatatgggc gtgatggcga
     5941 tggagtgttt tgtcaccccg caaggtctgc tgatcaacga actggctccg cgtgtgcata
     6001 acagcggtca ctggacacaa aacggtgcca gcatcagcca gtttgagctg catctgcggg
     6061 cgattaccga tctgccgtta ccgcaaccag tagtgaataa tccgtcggtg atgatcaatc
     6121 tgattggtag cgatgcgaat tatgactggc tgaaattgcc gctggtgcat ctgcactggt
     6181 acgacaaaga agtccgtccg gggcgtaaag tggggcatct gaatttgacc gacagcgaca
     6241 catcgcgtct gactgcgacg ctggaagcct taatcccgct gctgccgcca gagtatgcca
     6301 gcggcgtgat ttgggcgcag agtaagttca gttaagcagg tgctcatttg attgccggat
     6361 gcggcgtaaa cgccttatcc ggcttaccgc tccggtaccc attgtaggcc tgataagacg
     6421 cgtcaagcgt cgaatcaggc atggtgcacc gattgccgga tgctctcatc actacagcga
     6481 aatacaggtc cccgcttcgc ccgccagcgt ctcttcaatt cgcgataacg ccccaatcca
     6541 cgcgggttta ctacggcttc tgacataacc actcaccgcc gttaccttcg gccccatcga
     6601 accatcggct ttggcaaatg gcgctaactc atccggtgtg gcatggcgaa tggcacgttg
     6661 ctgcggcgtt ccccagtttt catataccgc atcagcatcg gtgaggatca ccagtccatc
     6721 ggcattaatc tgctcggcga gcaacgcagt ggcgagatct ttatcaatca ccgcttcact
     6781 ccctgcccca tcttccgtca caggcacacc gccgccgcca ctgcaaatca ccacatgccc
     6841 ctctttgagc aacaactcga tggcttcgct gtcgagaatt ttacgcggtt gcggagacgc
     6901 caccacccgg cgcaaatatt taccatcacg tttcatctgc cagccgtaag ccgcttccag
     6961 tgcctcttgt tcttctggct gataaaccgg accaataaat ttctctggct gcaaaaacgc
     7021 cggatcatca ggcgaaacct caatgcgcgt cagcaccgtc gtaacaggcg gtatctgcgg
     7081 ctgtgcgctc aagctctgcg ccagcatata gcctcatccc ctggctttcc gccaccagca
     7141 catccagcgg atacggttct acctctttcc acgccagatt ctgtaacgcc agcagcccca
     7201 cctgcggccc gttgccgtga acaatcgcca gccgataaga acgggccagg cgtgccagcg
     7261 cgggtacagc actggcgata ttgcgatatt gattttccgc cgtcagcgcc tcaccgcgct
     7321 ggagtaaggc gttgccccca agagctacaa ccagtgtttt catggttttc cttgtaataa
     7381 ttgttggcca agccagaacc ccagcagcgt atctgcccct gacgtatgcc ccagcgccag
     7441 cagcgaatca atcgcaacag cggtacgttt cgggcaactc agagcatgga caaagtgcag
     7501 gagtggcgag gcgaaatatc cttgcgcggc ataacgtaag taactgacgc tcaccgctgt
     7561 ggtaacgagt tgaagattgt cggaacaggc aaaaaacggg cgaccggcgc gcgcatctaa
     7621 agcgccataa taccaagccg ccagcagcat tccgctcaac gtgtcatcat gacttggcgt
     7681 taatccgggg cctttaccca gccagtgccg ccagtcggtc ttaacgccat tgagcgcggc
     7741 ctgaaaacag tgacgaaact ggcgtaattc agcggtcagc ggatcgcttg ccgccatcgc
     7801 cagtggtccg aaaagtccgg tttcctccgc gcgttgcatc catgcagctg caagcggttg
     7861 aggatgcgaa ggcggcgtaa tacgcagcaa acaataacgc tgtggctgtt taaccgtgaa
     7921 acgcccgagg cgaatccctt gagccacaac ctgtggtcgt tcattgccgc ataatccacc
     7981 gcataacgca tcgaattgcg cgcggcgaag cacccatcct ccggggccga aaccactacc
     8041 ctgacgatgc agcgttaaca gttcgccgct ttccgtcatc aggttaatcg cccgccgcca
     8101 cactcccgct aaccgccagg actggcgata attcggtgcg ctactgctgg caagcagagg
     8161 atggatgatc gtcattgcgc gttcctcaac caatccccat gctttcagcc agtgcttcca
     8221 gcgcctgttc aaagcacgcc agcggtgccc gcacggtgcc tgcgccaatc tgcccgatcc
     8281 ccgcctcttt atgggcgata ccggtattga tgagtggcgt aatgccggtt tctaccacgc
     8341 gacgaatatc cagccccagg cacgcgccct gaaaatccca gcctgggatc tgcaactgca
     8401 tattgcgttc aaggtaaatt tccgccatct cttcagatac cgccctggcc gcttccatgc
     8461 cacccgcacc gacaaagcgc gttacgccag gcgctgcgat cattgccgca cctccgatac
     8521 caaaggtttc ggtaatcgcg ctatcgccca tatccgggtt cgcctgctcc tgcgagaagc
     8581 cggtgaaaaa cagaccttgc ggagtgttta caggcgcagt aaaccagcgt tcacccagcc
     8641 cgcttacccg aatcccgaac atattgccgt tgcgggtcat agccgtaacg atgctgcctg
     8701 cgcggatcat cgcgccagca tccatcgccg ccttgcagta ggccatcgcg aggttgagga
     8761 agaactgatc ggtcacgctg aggaaatcga tcacttccgc aatgtgctgt ttatcatgat
     8821 cgaggcgagc aatttgtggg gccagcgtac gcattaacag tgctgaggaa gcaatattgc
     8881 gttgatggaa ctcatcgccc atcgtaatgc cctgcgccat catcgccgtg agatcgatac
     8941 cgtgctccat gcgccccagc gccgcgctta atactggcat taacacatcg cgcatccagc
     9001 gatggcgagt caggacatct tcgccgtaag cgccaaaacg catcactttg ccgatacctt
     9061 cgttgaggtt gcagtacgcc cggttgccgt cggtcacgtt ctcgaccacc agcatcggca
     9121 tactggcaga agtaatgccg cccattggcc cgacggcatt cacatggtga caaggaatga
     9181 agttcacttc cccctgctcc agcatcgcca gtgcctgcgc ttcatctttc gcccagcctt
     9241 cgaacagaca tccgcccacg cacgcccctt tcatgggtcc ggtcatctcc tgccagcgca
     9301 ttggcggccc ggcgtgaagc agtgttttgc cctcgtttag ttcgctgata agtgaaaaag
     9361 ccggttgcac atccagccag tgtggacgag cacgacgaat ttgttcgata accgcagcat
     9421 tggcttgcgc cactgatgta aacatgggaa ccccttattg caaacgttct aataaacgag
     9481 ccagtttttt attgccgccg gcgactggcg accattggta atgcacaacc ggtttgctgg
     9541 cgctttgtag ctccagcgca aagctgcgta atccgatgtt aatcacggcg acgttttcca
     9601 gtaatgacgg tgtatgttgc tgtgcggcag gcgagagcgg ataaattaac gctgccgcca
     9661 gcaaggtggc ttccggcagc gaactcacta ccgcgatccc cgcatcttcc agcgtggcga
     9721 tttgctgcga gcggcattgc gggtcacgtt cagtgcctgt caccgtggca atggcataca
     9781 gtggttgatt atctgaacgc gcggcacagg ctttttgcta ggcgcttacc agcgaggcgg
     9841 caggatcggc agtcgcaccg aagccaatca cgacatcaag tagcaatacg cgcacttgtg
     9901 gtttagcgcc gagatcggca attaactggt tgcgtaaggc tgggtcgatc atcggatggg
     9961 gacgcccgac ggtgtagaaa tcatcgccga ggtcaataat ctggtgacca tcggcgtcga
    10021 gcatcatgcc gtgatgatgg gtatcgtcgg cttccacgcc aaggtgtcca gcaagtaatc
    10081 ccgccgcttc ggcagccagc gtaccgccgg tatacaaacc gcaaataaat ccgctgctga
    10141 caggcgctat tgcgttacgt cgcgccgtga cgcgtgaaag caggcaagcc aggcgtgcgg
    10201 cctcatccag cgaggaggca aaccagacat tctcgtcgcg ggccaccgcc ggggtataac
    10261 ctaaaaacag cgccaccatc ggtttgccgg ttgctttcat ggcattaaca attttcagac
    10321 gcacagcttc gg
//
LOCUS       DAZPEB010000147        10291 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZPEB010000147 DAZPEB010000000
VERSION     DAZPEB010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10291
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.150"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..260)
                     /locus_tag="ACHURJ_003145"
     CDS             complement(<1..260)
                     /locus_tag="ACHURJ_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFR1281989.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAILRCTGNSGHYH"
     gene            complement(513..983)
                     /gene="bcp"
                     /locus_tag="ACHURJ_003146"
     CDS             complement(513..983)
                     /gene="bcp"
                     /locus_tag="ACHURJ_003146"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFR1281990.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(983..1555)
                     /locus_tag="ACHURJ_003147"
     CDS             complement(983..1555)
                     /locus_tag="ACHURJ_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFR1281991.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            1701..2579
                     /gene="dapA"
                     /locus_tag="ACHURJ_003148"
     CDS             1701..2579
                     /gene="dapA"
                     /locus_tag="ACHURJ_003148"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFR1281992.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            2596..3630
                     /gene="bamC"
                     /locus_tag="ACHURJ_003149"
     CDS             2596..3630
                     /gene="bamC"
                     /locus_tag="ACHURJ_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFR1281993.1"
                     /translation="MAYSVQKSRLAKVAGVSLILLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGVRTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTIDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSASDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            3843..4556
                     /gene="purC"
                     /locus_tag="ACHURJ_003150"
     CDS             3843..4556
                     /gene="purC"
                     /locus_tag="ACHURJ_003150"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFR1281994.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKRFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            4724..5587
                     /locus_tag="ACHURJ_003151"
     CDS             4724..5587
                     /locus_tag="ACHURJ_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFR1281995.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHNMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            5602..7617
                     /gene="tmcA"
                     /locus_tag="ACHURJ_003152"
     CDS             5602..7617
                     /gene="tmcA"
                     /locus_tag="ACHURJ_003152"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFR1281996.1"
                     /translation="MAELTALYTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALL
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQYFLQAAGENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGVGQQLIASALQYTQDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYCLRRDAQALAQWNGETLPVD
                     PLNDIVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLAGRKMLLVRQREEAAHALFALNDVRTERLRDRITQWQFFH"
     gene            7691..8389
                     /gene="ypfH"
                     /locus_tag="ACHURJ_003153"
     CDS             7691..8389
                     /gene="ypfH"
                     /locus_tag="ACHURJ_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR1281997.1"
                     /translation="MKHDHFVVQSPDKPVQQLLLLFLGVGDNSVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(8499..8699)
                     /locus_tag="ACHURJ_003154"
     CDS             complement(8499..8699)
                     /locus_tag="ACHURJ_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFR1281998.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(8727..9854)
                     /gene="dapE"
                     /locus_tag="ACHURJ_003155"
     CDS             complement(8727..9854)
                     /gene="dapE"
                     /locus_tag="ACHURJ_003155"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFR1281999.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(9858..10214)
                     /locus_tag="ACHURJ_003156"
     CDS             complement(9858..10214)
                     /locus_tag="ACHURJ_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFR1282000.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPSIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
BASE COUNT         2459 a         2546 c         2806 g         2480 t
ORIGIN      
        1 aaatggtagt gtccgctatt gccagtacac ctcaggatcg catcatccct ctgggagatg
       61 gcattgaccg ggcagacctg caggcaaggg gcttcctcac agtgatgaca cacgacaggg
      121 gcagtgattg ttgacgtctt cgccagggcc aggcgcgggt gttgctgtaa accttgcgtt
      181 ttatgcacgt ccgaacaggc agcgagacag gtattacatc ctgtacacca cagtggttcg
      241 gccaccacaa agcggttcat gtcggctcct tgaaaatgat atgaaacatt gcatttcata
      301 ttgcataaag gatgccagtc tgattaattt aatatattca ataaattatg tattaacagg
      361 gttctgaacg gtacaaaaat gatggtcatt aacgttcagc aaaaaatgga ttcgacaccc
      421 gtgtcgcagg ggaaagcgag aatgtgatct tgatcacgag aagtgaggtg tgctgaccgc
      481 aagcgcagcc agcacggaat ggagcaaagt aatcaggcgt gttctttcag ccagttcagc
      541 acaacgtcgt ggtgattgct ggttttaaaa tcgtcaaaga catgttcgat tttgccatca
      601 gcgtcaatca ggaagctgat gcgatgaata ccatcgtagg ttttgcccat gaaggacttt
      661 tcaccccaga cgccgaattg ttcgcacacc tggtggtcct catcagacag gagcgtaaag
      721 ttaagcagct ctttttccgc aaaacgggag agtttttcgg gtttatcggt gctgataccc
      781 agcacatcaa cgcccgcttt tttcaactca tccatgttat cgcgtaagcc gcaggcctgt
      841 acggtacagc cgggggtcat ggctttcggg tagaaataaa ccagaacacg ctgtccctgg
      901 aagtcggtca aattaacttg ttctccgtct tgatccggca agctaaattt cggtgcgata
      961 tcaccggctt tcagtggatt cattacttaa ctccatcctg ttcatcatgt tgggaataat
     1021 tgacgacgtt aatactgcct tgtgcattga gttctgtaca tagggctttg aacgcttgct
     1081 caatatttgc tgcgtccgca gatgcggggc tgtgggcggt gatctgaata tgcaactgcg
     1141 cagcccgttc attttcagca ggttgcgtgc gcgacaccag ctccgcaatg ttcatatgat
     1201 gcgcgtcgaa aagtgctgtg aagcgttcaa ttaaatgcgg ggagtctgca acatcgacct
     1261 gaacccagac agatgctggc attggcggac gcggacgcgc cgtcgtgcgc ttcatcacga
     1321 ttaaaagatc cagttcggca cctttcaacg gtaacgttga ttcaatcaga gtaatggcat
     1381 tccatgaacc ggaaagcagc atgataaacg tgaactcttc tcccagcatc gccaggcgac
     1441 tgtcttcaat attacagccg caactactga catgacgggt gatggtgttc acaattccag
     1501 ggcgatcggc acccaacgca gtgatcacca gataatgttg cgatgacagt gtcaaactgg
     1561 ttattccttt aaggggtgag ttgttcttaa ggaaagcata aaaaaaacat gcatacaaca
     1621 atcagaacgg ttctgtctgc ttgcttttaa tgccatacca aacgtaccat tgagacactt
     1681 gtttgcacag aggatggccc atgttcacgg gaagtattgt cgcgattgtt actccgatgg
     1741 atgaaaaagg taatgtctgt cgggctagct tgaaaaaact gattgattat catgtcgcca
     1801 gcggtacttc ggcgatcgtt tctgttggca ccactggcga gtccgctacc ttaaatcatg
     1861 acgaacatgc tgatgtggtg atgatgacgc tggagctggc tgacgggcgc attccggtga
     1921 ttgccgggac cggtgctaac gctactgcgg aagccattag cctgacgcag cgcttcaatg
     1981 acagcggtat cgtcggctgc ctgacggtaa ccccttacta caatcgtccg tcgcaagaag
     2041 gtttgtatca gcatttcaaa gccatcgctg aacatactga cctgccgcaa attctgtata
     2101 atgtgccgtc ccgtactggc tgcgatctgc tcccggaaac ggtgggccgt ctggcgaaag
     2161 taaaaaatat tatcggaatc aaagaggcaa cagggaactt aacgcgtgta aaccagatca
     2221 aagagctggt ttcagatgat tttgttctgc tgagcggcga tgatgcgagc gcgctggact
     2281 tcatgcaatt aggcggtcat ggggttattt ccgttacggc taacgtcgca gcgcgtgata
     2341 tggcccagat gtgcaaactg gcagcagaag ggcattttgc cgaggcacgc gttattaatc
     2401 agcgtctgat gccattacac aacaaactat ttgtcgaacc caatccaatc ccggtgaaat
     2461 gggcatgtaa ggaactgggt cttgtggcga ccgatacgct gcgcctgcca atgacaccaa
     2521 tcaccgacag tggtcgtgag acggtcagag cggcgcttaa gcatgccggt ttgctgtaaa
     2581 gtttagggag atttgatggc ttactctgtt caaaagtcgc gcctggcaaa ggttgcgggt
     2641 gtttcgctta ttttattact cgctgcctgt agttctgact cacgctataa gcgtcaggtc
     2701 agtggtgatg aagcctacct ggaagcggca ccgcttgcgg agcttcatgc cccggctgga
     2761 atgattttgc cggtgacctc cggtgattat gcaatcccgg tgaccaacgg tagtggtgct
     2821 gtcggtaagg cgctggacat tcgtccacca gcccagccgc tggcactggt ttctggcgtg
     2881 cgtacccagt tcacgggcga taccgcttca ttgctggtgg aaaatggtcg tggcaatact
     2941 ctgtggccgc aggtggttag cgtgctgcag gcgaaaaact acaccatcac ccaacgtgat
     3001 gatgctggtc agacactgac catcgattgg gtacaatgga accgtctgga cgaagacgag
     3061 cagtatcgtg gtcgttatca aatctctgtt aagccgcagg gttatcagca ggcggttacg
     3121 gttaaactgc tgaacctgga acaggcgggc aaaccggttg cagacgcggc ttccatgcag
     3181 cgttacagca cggagatgat gaacgttatt tccgccggtc tggataaatc tgcctctgac
     3241 gccgcgaacg ctgcgcaaaa tcgtgcctcc accactatgg acgtacaaag tgcagctgat
     3301 gacaccggtt taccgatgct ggtcgtacgc gggccgttca atgtggtttg gcaacgtctg
     3361 ccagcggcgc tggaaaaagt gggcatgaaa gtgaccgaca gcacccgttc gcagggtaac
     3421 atggccgtaa cttataagcc gctgtctgac agcgactggc aggaactggg cgcgagcgat
     3481 ccaggcctgg catccggtga ctataaattg caggttggcg atttagataa ccgcagcagc
     3541 ctacagttca tcgatccgaa aggtcatact ctgactcaga gtcagaacga cgcgctggta
     3601 gctgtcttcc aggctgcgtt tagcaagtaa aaatataggg ctggagtcat ccggcccttt
     3661 tttctgatat gatacgcaaa cgtgtgcgtc ggcagaaaaa tgcaatttta gcggtaattc
     3721 gcacgaaatt tgtttgtcgg acgtaggtcg gataaggcgt tcttgccgca tccgacaaaa
     3781 catccggcac accagacagc aaaagatttt aaaacgttaa ttcacaccca ggagtgataa
     3841 agatgcaaaa gcaagctgag ttgtatcgtg gtaaagcgaa aaccgtatac agcacggaaa
     3901 acccggacct gttggtgctc gaattccgca atgatacgtc agcaggggat ggcgcgcgca
     3961 ttgagcagtt tgatcgcaaa ggtatggtga acaacaagtt caactacttc attatgagca
     4021 aactggctga agcgggtatc ccgactcaaa tggagcgtct gctctccgat accgaatgtc
     4081 tggtgaaaaa gctggatatg gtgccggttg agtgtgtcgt gcgtaaccgt gctgctggct
     4141 ctctggtgaa acgtcttgga atcgaagaag gtattgagct gaacccgccg ctgttcgatc
     4201 tgttcctgaa aaacgatgcc atgcacgatc cgatggtcaa cgaatcttac tgcgaaacct
     4261 ttggctgggt gagcaaagag aacctggcgc gtatgaaaga gctgacctac aaagcgaacg
     4321 acgtgctgaa aaaacggttc gatgatgctg gtctgattct ggtcgacttc aagctggagt
     4381 ttggtctgta caaaggtgaa gtggtactgg gcgatgagtt ctccccggac ggcagccgcc
     4441 tgtgggacaa agaaacactg gagaaaatgg acaaagaccg tttccgccag agcctcggtg
     4501 gcctgatcga agcctatgaa gccgtcgccc gccgcctggg tgtacagctg gactgatttt
     4561 tctgttcatc atcttgccgt gcagctggca cggcaagaca accgctctcg gaatatatgc
     4621 attgaacgct cattcctccg ccatttcatc ccgctttcct gtggtaatct tcctctgaac
     4681 cacctacgat catttctata atgaatatat tgttgaggta tctatgcgtt ggcaagggcg
     4741 acgtgaaagt gacaatgttg aagacaggcg caacagctcc ggtggtccgt ctatgggcgg
     4801 tcccggtttt cgcctgccaa gcggtaaagg cgggctgatt ttactgatag tcgtgctggt
     4861 tgcaggctac tatggtgttg atttaaccgg gttaatgact ggacagccgg tttcccaaca
     4921 acaatcaacg cggtccatta gccctaatga agacgaagct gcaaaattca cctcggtgat
     4981 tctggcaacc acggaagaca cctggggaca acagttcgag aagatgggta agacctatca
     5041 gcaaccgaag ctggtcatgt accgtggaat gacgcgcacc ggctgcgggg cgggccagtc
     5101 cattatgggg ccgttctatt gcccggcgga tggcacagtt tatatcgatc tctccttcta
     5161 tgatgacatg aaagacaaac ttggcgcgga tggcgatttt gcccaggggt acgttatcgc
     5221 ccatgaagtc ggtcatcatg tgcagaaact gttaggcatc gagccgaaag ttcgtcaact
     5281 gcaacaaaac gcgacgcagg cggaagtgaa ccgcttatct gtgcgtatgg aactccaggc
     5341 cgactgtttt gccggtgtct gggggcataa catgcagcag caaggcgttc tggaaaccgg
     5401 cgatctggaa gaggcgctga atgcggcgca ggccatcggc gatgaccgtt tacaacagca
     5461 aagtcagggg cgagtagtac cagacagttt cacacatggc acttctcagc aacgctacag
     5521 ctggtttaaa cgtggtttcg acagcggcga tccggcacaa tgcaatactt ttggtaaaag
     5581 catttaattt ccggggcagg gatggctgaa ctgactgcgc tttacacatt aacagcgcaa
     5641 atgaaacgtg aagggatccg ccgcttgctg gtgttgagcg gggaagaggg ttggtgtttt
     5701 gatcatgcgc ttaagttgcg tgatgcctta cttggcgact ggctgtggat ttcgccgcag
     5761 ccagatgctg aaaaccactg ttctccctcg gcgctacaaa ctttacttgg gcgcgagttc
     5821 cggcatgcgg tattcgacgc ccgccacggc tttgatgccg ctgcctttgc ggcacttagc
     5881 ggaacgttga aagcgggaag ctggctggtt ttgttactcc ctgtatggga agagtgggaa
     5941 aaccaacctg atgccgactc gctgcgctgg agtgattgcc ctgaccctat tgcgacgccg
     6001 cattttgtcc agcatctcaa acgcgtactt acggcggata acgacgctat tctctggcgg
     6061 caaaaccagc cgttctcgtt ggcgcatttt actccccgta ctgactggca ccccgcgacc
     6121 ggcgcaccac aaccagaaca acagcaactc ttacagcagc tactgaccat gccgccgggc
     6181 gtggcagcgg taacggctgc gcgtgggcgc ggtaagtcgg cgctggcagg gcaactcatt
     6241 tctcgtattg cgggcagtgc gattgtcacc gcgcccgcaa aagcggcaac ggatgtactg
     6301 gcacaatttg cgggcgagaa gtttcgcttt attgcgccgg atgccttgtt agccagcgat
     6361 gagcaagccg actggctggt ggtcgatgaa gccgcagcca tacctgcgcc gttgttgcat
     6421 caactggtat cgcgttttcc tcgaacgttg ttaaccacta cggtgcaggg ctacgaaggc
     6481 accggacgtg gttttttgct gaaattttgc gctcgctttc cgcatttaca ccgttttgaa
     6541 ctgcaacagc cgatccgctg ggcacaggga tgcccgctgg aaaaaatggt tagtgaggca
     6601 ctggtttttg acgatgaaaa cttcacccat acaccacaag gcaatatcgt catttccgca
     6661 tttgaacaaa cgttatggcg aagcgagcca gaaacgccgt taaaggttta tcagttattg
     6721 tctggtgcgc actaccggac ttcgccgctg gatttacgcc ggatgatgga tgcaccaggg
     6781 caatattttt tacaggcggc tggcgaaaac gagattgccg gagcgctgtg gctggtggat
     6841 gaggggggat tatctcaaca actcagtcag gcggtatggg caggttttcg tcgcccgcgg
     6901 ggtaatctgg tggcccagtc gctggcggcg cacggtagca atccactggc agcgacattg
     6961 cgtggacggc gggtcagccg gatagcagtt catccggctc gtcagcgcga aggcgttggg
     7021 caacagctca ttgccagcgc tttgcaatat acgcaagacc tcgactatct ttcggtgagt
     7081 tttggttaca ccggggagtt atggcgtttc tggcaacgct gcggttttgt gctggtacga
     7141 atgggtaatc atcgtgaagc cagcagtggt tgctatacgg cgatggcgct gttaccgatg
     7201 agtgatgcgg gtaaacagct ggctgaacgt gagcattact gtttacgtcg cgatgcgcaa
     7261 gctctcgcgc agtggaatgg cgaaacactt cctgttgatc cactaaacga tatcgtgctt
     7321 tctgacgacg actggcttga actggccggt tttgcttttg ctcatcgtcc gctattaacg
     7381 tcgttaggtt gcttattgcg tctgctacaa accagtgaac tggcattacc ggcgctgcgt
     7441 gggcgtttac agaaaaatgc cagtgatgcg cagttatgta ccacacttaa acttgcaggc
     7501 cgcaagatgt tactggtccg tcagcgggaa gaggccgcgc atgcgctgtt cgcacttaat
     7561 gatgttcgca ctgagcgtct gcgcgatcgc ataacgcaat ggcaattttt tcactgactc
     7621 cttcaatttt ctcccatggt cattgtgagc cagcggcgta aaattaatga catcagttaa
     7681 ggagttcgcc atgaaacatg accattttgt tgttcaaagc ccggataaac ctgtgcaaca
     7741 gttgttactg cttttcctcg gtgtcgggga taactcggtg gcaatggggg aaataggcag
     7801 ttggtttgca ccgctgtttc ctgatgcgct ggtggtgagt gtcggtggcg cggagccgag
     7861 cggtaatccg gcggggcgtc agtggttttc ggtgcagggt attacggaag ataatcgcca
     7921 ggcgcgtgtg gatgccatca tgccgacgtt tattgaaacg gtacgctact ggcagaaaca
     7981 gagtggagta ggggctaacg ccacggcgtt aatcggtttc tctcaaggcg cgattatggc
     8041 gctggagagt attaaagccg aaccgggcct tgcttcacgc gtcattgctt ttaacggacg
     8101 ctatgcgagc ctgccggaaa ccgcgtctac agcaacgaca attcacctga ttcatggcgg
     8161 tgaagatccg gttatcgatc tggcgcatgc ggttgcggcg caggaagcgt taatcagtgc
     8221 cggtggtgac gtgacgctgg atatcgtgga ggatttaggc catgcgatcg acaatcgcag
     8281 tatgcaattt gccctggatc acttgcgtta taccattccg aaacattatt tcgacgaagc
     8341 gctaagtggc ggtaagcctg gcgatgatga tgtgattgaa atgatgtaaa agacagatta
     8401 cctggcctga taagacgcgt gagcgtcgca tcaggcatcg ttcaccattg ccggatgcgg
     8461 cgttaacgcc ttatccggcc tacggttgag cactcagctt acttcttcgg ttgatccttt
     8521 ttcggccagt cgtcgtcatc gtcccactta tcgttgaaat cacgatgcgg gggaagctcc
     8581 ggcttgttgg cgagaaattt cttgtggtcg acgcgcttga gatctttaat cacgttcagc
     8641 aggacgccta ccaaaaagac aatcaccaga atccaccaat atttagccag ccagtccatg
     8701 cttatttcct cttgcagaac cactcatcag gcgacgagct gttccatgat acgttgatac
     8761 atacgggcaa gtagctgcag gtcggcagcg ttcacacatt cattaatttt atgaatagtg
     8821 gcattgaccg gcccgagttc caccacctgc gcccccatgc gagcaataaa gcgcccgtcg
     8881 gacgttccgc ctgtggtcag tagctgcggt ttaatttcat tatagtgctc aaccgcgtta
     8941 acgaccgcat ccaccagttt accgcgcgcg gtcaaaaatg gctgcccgga aagccaccaa
     9001 tccaccgtat agcgcagttg atgtttttca agcagggcaa gcacctgcgc tttgatcatc
     9061 tcatcagtca gttcggtgct gaagcggaag ttaaactgca caaacagttc acccggaata
     9121 acattgttac tgcccgttcc cgcctgaata ttagcaatct gcatactggt cgccgggaaa
     9181 aattcattgc cctgatccca ctcaatagcc actaattcat taaggaaagg tgctgcgcga
     9241 tgtactggat tgtcagccag atgcgggtag gccacatgcc cctgaacgcc atgaatggta
     9301 aggttgcagg ttaacgatcc gcgacgacca tttttcacca catcaccaac cacttcgata
     9361 ctcgacggtt cgccgaccag gcagtaatcg agacgctcat tacgtgccat caacgcttcg
     9421 acgactttta ccgtaccgtt gtgggcactg gcttcttcat cagaggtgat cagaaatgcc
     9481 agtcgccccg tatggttggg atgttgtgcg acaaagcgtt ctgccgctac caccatcgcc
     9541 gccagcgagc ctttcatatc tgccgcaccg cgcccgaata acatgccatc acgaatggtg
     9601 ggttcaaacg gcggattgat ccaacgatcg gcgtcgccag gcggcaccac gtcggtatgc
     9661 ccggcaaagg ctaacgtttc accttgccca cgccatgccc aaaaattctg cgtatcggca
     9721 aagtccatgc gttcaacggt aaaaccgatc gcctgcaaac gttcaatcaa caaagcctgg
     9781 catcctgcgt catcaggact cagggaaggg cggcgaataa gctgttgtgt cagctcaata
     9841 accgggcacg acatagacta cacctcatgg aaaaattgct gataactgga atcactgaaa
     9901 cccagcagca taggcttacc aggcgcgcag agcaatggac gtttgataat tgaaggcatt
     9961 tcagtcatta atgccgccgc agaggccgca tcggtgattt tattgcgggt ggtttcgtcc
    10021 agtttacgcc aggttgtacc acgggtgttc agtaacgctt cccagcctaa ttcgttgata
    10081 aaaccgttca ataattcgct gtccagccca tcgacgcggt aatcatgaaa acgatagtcg
    10141 atgttattgg cttccagcca acggcgagcc tttttaatgg tgtcacaatt tttgatgccg
    10201 taaagtgtaa ccataatgaa tcctttttcg atctatgaga ataaatactc atttaagggt
    10261 gaaaacattt tgcctgcaaa tgattatcag g
//
LOCUS       DAZPEB010000148        10272 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZPEB010000148 DAZPEB010000000
VERSION     DAZPEB010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10272
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.151"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            75..650
                     /gene="ssuE"
                     /locus_tag="ACHURJ_003157"
     CDS             75..650
                     /gene="ssuE"
                     /locus_tag="ACHURJ_003157"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFR1282001.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFVP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHRPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            643..1602
                     /gene="ssuA"
                     /locus_tag="ACHURJ_003158"
     CDS             643..1602
                     /gene="ssuA"
                     /locus_tag="ACHURJ_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFR1282002.1"
                     /translation="MRNIIKLALVGLLSVSTIAVAAESSPEALCIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1599..2744
                     /gene="ssuD"
                     /locus_tag="ACHURJ_003159"
                     /pseudo
     CDS             1599..2744
                     /gene="ssuD"
                     /locus_tag="ACHURJ_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
     gene            2809..3546
                     /gene="ssuC"
                     /locus_tag="ACHURJ_003160"
                     /pseudo
     CDS             2809..3546
                     /gene="ssuC"
                     /locus_tag="ACHURJ_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001500727.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
     gene            3543..4294
                     /gene="ssuB"
                     /locus_tag="ACHURJ_003161"
                     /pseudo
     CDS             3543..4294
                     /gene="ssuB"
                     /locus_tag="ACHURJ_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309043.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
     gene            complement(4337..6949)
                     /gene="pepN"
                     /locus_tag="ACHURJ_003162"
     CDS             complement(4337..6949)
                     /gene="pepN"
                     /locus_tag="ACHURJ_003162"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFR1282003.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPEHFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVISLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQFHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7215..8417
                     /gene="pncB"
                     /locus_tag="ACHURJ_003163"
     CDS             7215..8417
                     /gene="pncB"
                     /locus_tag="ACHURJ_003163"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFR1282004.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYKKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            8586..9986
                     /gene="asnS"
                     /locus_tag="ACHURJ_003164"
     CDS             8586..9986
                     /gene="asnS"
                     /locus_tag="ACHURJ_003164"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFR1282005.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2314 a         2652 c         2767 g         2539 t
ORIGIN      
        1 ttgtctctcc gacagactga ccgaattgtt atcaatgtta acaaaaaaag aacaattggt
       61 tataaggaga gagtatgcgt gtcatcaccc tggcgggtag tcctcgcttt ccttctcgct
      121 ccagctcctt gctggaatat gcgcgggaaa aactaaatgg cctggatgta gaggtttatc
      181 actggaatct gcaaaacttc gtcccggaag atctgcttta tgctcgtttc gatagtccgg
      241 cactcaagac cttcaccgaa cagctgcaac aggctgatgg actgatagtc gctacacctg
      301 tgtataaagc cgcctattcc ggcgcgctga aaaccctgct cgacctgctg ccagagcgcg
      361 ctttgcaagg caaagtggtg ctaccgctgg cgacgggcgg taccgtggcc catctgctgg
      421 cggtcgatta tgcccttaaa ccagttttaa gcgcactgaa agctcaggag atcctgcacg
      481 gcgtgtttgc cgatgactca caagtaattg attaccatca cagaccccag ttcacgccaa
      541 atctgcaaac ccgtcttgat accgcgctag aaactttctg gcaggcattg caccgccgcg
      601 atgttcaggt tcctgacctt ctgtctctgc gaggtaatgc ccatgcgtaa catcattaaa
      661 ctggcgctgg tgggattgct tagcgtctct acgattgcgg ttgctgcaga atcctcgcct
      721 gaagcgttat gtataggcta tcagaaaggc agtattggta tggtactggc aaaaagccac
      781 cagttactgg aaaaacgcta tccgcaatca aaaatctcct gggtggagtt ccccgcgggt
      841 ccgcaaatgt tggaagcctt aaacgttggc agtattgatc tcggcagtac cggggatatt
      901 ccgccaatct ttgcccaggc tgccggggct gatttggtat atgtgggcgt cgagccaccg
      961 aagcccaaag ccgaagtgat tctggtggca gaaaacagcc cgatcaaaac cgtagccgat
     1021 cttaaaggtc acaaagttgc ctttcagaaa ggttccagtt cacacaacct tttactgcgt
     1081 gcactgcgtc aggccggact taagtttacc gatatccaac ccacttacct gacgcccgct
     1141 gatgcccgcg ccgcgttcca gcaaggtaac gttgacgcct gggctatctg ggatccctac
     1201 tactctgctg cattattaca gggcggcgtg cgggtgctga aagacggcac cgatctcaat
     1261 caaactggat cgttttatct ggcagctcgt ccctatgcag aaaaaaacgg cgcttttatt
     1321 cagggcgtac tggcaacctt tagtgaggcc gatgcgttaa cccgcagcca gcgcgagcaa
     1381 agcatcgctt tactggcaaa aacgatgggc ttaccggcac cggtgattgc ctcttactta
     1441 gatcatcgcc ctcctaccac catcaaaccg gttaacgccg aggttgccgc cttacagcag
     1501 caaacggcag atctgtttta tgaaaatcgt ctggtgccga aaaaagtcga tattcgccag
     1561 cgcatctggc agcccactca actggaagga aaacaattat gagtctgaat atgttctggt
     1621 ttttaccgac ccacggtgac gggcactatc tgggaacgga agaaggttca cgcccggttg
     1681 atcacggtta tctgcaacaa attgcgcaag cggcggatcg tctgggctat accggtgtgc
     1741 tgatcccaac ggggcgatcg tgtgaagatg cgtggctggt ggcggcgtcg atgatcccgg
     1801 tgacgcagcg gctgaagttt cttgtcgccc tgcgccccag cgtaacctca cctaccgttg
     1861 ccgctcgcca ggccgccacg cttgaccgtc tctcaaatgg tcgtgcgttg tttaatctgg
     1921 tcacaggcag cgatccacaa gagctggcag gcgacggcgt gttccttgat catagcgagc
     1981 gctacgaagc ctcggcggaa tttacctagg tctggcggcg cttattgctg ggggaaaccg
     2041 tcaatttcaa cggtaaacat attcatgtgc gcggagcaaa actgctcttc ccgccgattc
     2101 aacagcctta tcctccgctt tactttggcg gatcgtcaga cgtcgcccag gagctggcgg
     2161 cagaacaagt tgatctctac ctcacctggg gcgaaccgcc agaactagta aaagagaaga
     2221 tcgaacaagt gcgggcgaaa gctgccgcgc atggacgcaa aattcgtttc ggtattcgtc
     2281 tgcatgtgat tgttcgtgaa actaacgacg aagcgtggca ggccgccgag cggttaatct
     2341 cgcatcttga tgatgaaact atcgccaaag cacaggccgc attcgcccgg acggattccg
     2401 taggacaaca gcgaatggcg gcgttacata acggcaagcg cgacaatctg gagatcagcc
     2461 ccaatttatg ggcgggcgtt ggcttagtgc gcggcggtgc cggaacggcg ctggtgggcg
     2521 atggtcctac ggtcgctgcg cgaatcaacg aatacgccgc gcttggcatc gacagctttg
     2581 tgctttcggg ctatccgcat ctggaagaag cgtatcgggt cggcgagttg ctgttcccac
     2641 atctggatgt cgccattccg gaaattcccc agccgcagcc gctgaatccg caaggcgaag
     2701 cggtggcgaa tgattttatc ccccgtaaag tcgcgcaaag ctaaggaaga aataatggca
     2761 acgccagtga agaagtggtt attgcgcgtt gccccctagt ttttaccggt gggcatcgtg
     2821 gcggtgtggc aactggcctc ctcagttggc tggctgtcga cgcgtatttt gccctcaccg
     2881 gaaggggtgg tgacggcgtt ctggacgctc tccgccagcg gcgaactgtg gcagcatctg
     2941 gcgatcagtt cctagcgggc gctgattggc ttttcaattg gcggatcgct ggggttgatt
     3001 ttgggactga ttagcgggct atctcgctgg ggagaacggc tgctggatac ctcaattcag
     3061 atgttgcgca acgtgccgca tctggcgctg atcccgctgg tgattttgtg gtttggcatt
     3121 gatgaatccg caaaaatctt tctggtggcg ctcggtacgc tgtttcccat ttatatcaac
     3181 acctggcatg gcatccgtaa tatcgatcgc gggctggtgg agatggcgcg tagctatggg
     3241 ttatccggca taccgctgtt tatccatgtg atcctgcctg gtgccctgcc ctcaattatg
     3301 gtcggcgtgc gttttgcgtt gggcctgatg tggctgacgc tgattgttgc cgaaaccatt
     3361 tctgccaatt ccggcattgg ttatctggcg atgaatgcgc gagagttttt gcaaacggac
     3421 gtggtggtag tcgctattat tctttacgcc ctgctcggca aacttgctga cgtcagcgcg
     3481 cagctgttag agcgcctctg gttgcgctgg aacccggctt atcatttgaa ggaggccacg
     3541 gtatgaatac tgctcgtctg aaccagggca caccattgtt gctcaatgca gtaagcaaac
     3601 attacgcgga aaatatcgtc ctgaaccaac tggatttaca tattccggca ggtcagtttg
     3661 tggcggtggt ggcaaaagta ccctgctgcg cctgctggcc gggctggaaa cgccaaccgc
     3721 aggcgatgtg ttggcaggca ccacaccgct ggctgaaatt caggaagaca cgcgaatgat
     3781 gtttcaggat gcgcgtctgc taccgtggaa atcggtgatt gataacgttg ggttaggcct
     3841 taaaggccag tggcgcgatg ccgcgcgtcg ggcgttggct gcggtaggac tggagaatcg
     3901 cgccggggaa tggcctgccg cactttcagg cgggcagaaa cagcgagtgg tgctggcaag
     3961 agcgttgatt catcgaccgg gtttattgtt gcttgatgaa ccgctcgggg cactggacgc
     4021 cttaacgcga ctcgagatgc aggatttgat tgtgtcactt tggcaggagc acggctttac
     4081 cgtactgttg gtgacgcatg atgtgagcga agcggtagca atggctgacc gggtgttatt
     4141 aattgaagag ggaaaaattg gtctggattt gacagtggat atcccccctc cgcgtcgatt
     4201 agggtcggtg cgattagcgg agctggaatc ggaagtgttg caaagggtga tgcagcgtgg
     4261 tgaatctgaa attcgcctga gaaaacaggg gtaaattccc cgaatggcgg cgctattgcc
     4321 gccattcggt tatttatcaa gccagtgctt tggtgatctt ctcgtacaga tcgccagaga
     4381 gattttccag ccctttcaac tgttccagcg ccgcgcgcat tttctcctga cgtttggcat
     4441 cataacgttt caggcgaatc agcggttcaa tcagacgtga agccacctgc gggttacggc
     4501 tgttgaggtc ggtaagcatt tccaccagga actggtaacc gctcccatct tcggcatgga
     4561 acgctgccgg attgctgccc gcaaacgcgc caatcaacga acgaatacgg ttcgggttgc
     4621 tcatggtaaa tgagcgatgc tgcaacagac cgcgcaccgt ctccagcaca ttcgccgccg
     4681 ggctggtggc ttgcaggata aaccatttat ccatcaccag accgtcctga tgccacttat
     4741 cgtcgtattc ctgcatcagc gcatcacggc atggcagctg tgcggcaacc gccgcagaaa
     4801 gcgccgccag cgcatcggtc atattgttcg cttcgtggaa ctgcttgctc accagcacgt
     4861 cagccagatg cgtttcacca aaagcaagga agcgcaggca ggcattacgc agagtgcgtt
     4921 tcgcaatatc ttcatgctca acacggtact cgctctggta attcgcgttg taaatagcca
     4981 gcagctcatc cgccagttca gtcgccagag tacgagtgag tgcttcgcgt acttcggcaa
     5041 tagcaatcgg gtcgatgata tcgaacagtt cagccatttc attgacagaa ggcagcgtca
     5101 ggatttctgc cgccagcgcc ggatcaatct tctcatcgag cagcactgcg cggaaagcgt
     5161 cagccacatg taccggcaga gacagcggct gcccttgctg atgtcgggcg acgttcagct
     5221 tgatgtaggt tgccagcagg ctttgtgccg catcccagcg ggagaaatca ttacgcgcat
     5281 gacgcatcag gaaggtcagt tgctgatcgc tccacttata ttccagtttc actggcgcag
     5341 agaattcgca cagcagcgca ggcaccggct ggaagtagac attatcaaaa acaaaggtct
     5401 gttccgcctg ggtgacgttc agcacggaat tcaccggatg accgcctttc tgcaacgaga
     5461 tcactttgcc ttcgttgtca tacaattcga tggcaaacgg aatatgcagc ggctgttttt
     5521 ctgcctgatc cggcgtggct ggcgtgcgct ggctgatggt cagggtgtac tgctcggttt
     5581 ccggattgta gtcgtctttg acggtcacaa tcggcgtgcc ggactggctg taccaacggc
     5641 ggaaatggga gagatcgaca ttcgacgcat cttccatcgc ctgcacaaag tcgtcgcagg
     5701 ttgccgcgct gccatcatgg cgctcgaaat agagttgcat ccctttctgg aagttttctt
     5761 cgcccagcag ggtgtggatc atacggatca cttccgcgcc cttctcgtaa acggtcaggg
     5821 tatagaagtt gttcatctca atgaccatgt ccgggcggat cgggtgcgcc atcgggctgg
     5881 cgtcttccgc aaactgcaat ccgcgcatgg tgcgcacatt attgatgcgg ttaactgcac
     5941 gggaaccaag gtcagagctg aattcctgat cgcggaagac ggttaaacct tctttcaggc
     6001 tgagctggaa ccagtcgcgg caggtgactc ggttaccggt ccagttatgg aaatattcat
     6061 ggccgataac gcgttcaata tcgaggtaat ctttgtcagt ggcggtgtcg gtgcgggcca
     6121 gcacatattt ggagttaaag atattcaaac ccttattctc cattgcgccc atattgaaga
     6181 aatccaccgc gacgatcata tagatgtcga ggtcatactc caggccgaag cgttcttcat
     6241 cccatttcat tgagtttttc agcgaggtca tcgcccacgg cgcgcgatca aggttgccgc
     6301 gatcgacgta cagctccagc gccacttcgc gaccagaacg cgtggtaaag gtgtcgcgca
     6361 gtacatcaaa gtcgcctgcc accagcgcaa acaggtagca cggtttcggg aacgggtcct
     6421 gccactgtac ccaatggcgt ccgttttcca gttcaccttg cgcaacgcgg ttaccgttgg
     6481 aaagcaggaa gggatatttg gttttatcgg caataatttt ggtggtaaaa cgcgccagca
     6541 cgtccgggcg gtcgagataa tacgtaatat ggcggaaacc ttcggcttca cactgggtgc
     6601 aaagcgcatc gcctgactga taaagccctt ccagcgcggt attcgccgcc gggcttattt
     6661 cattaacgat cttaagcgta aaatgctccg gcaaattact gatgaccagt gcgccctctt
     6721 cttctttcca ggcggtccac ggctcatcat taatatgaac agaaaccagt ttgaggtctt
     6781 cgccgttgag acgaagcgga gcatctgatg caccatgacg gacagcctgg ctgaccgcgg
     6841 taacgaccgt cttttgcgcg tcgaggtcaa aggtcaagtc aatatcagta atctggtaat
     6901 ccggcgcacg ataatcgtga cggtatttgg cttgtggctg ttgagtcata aataaccttt
     6961 agcatctttt atagagtctg gtgttcagtc tattcctgtt gcgtaaatcg cgctatgcag
     7021 aatcttcatc ttttcaggta caaacgcctt tattgctaca tttttataac atacaccgcg
     7081 taatgccatc gaccagaaag gtggcatatg gtgtgatcgg ggttcaataa attgcgaaac
     7141 aaggtatact ccagcagttc ctgaagatgt ttattgtact aaacgctcct gtacgaggat
     7201 gctactgcgc acctatgaca caattcgctt ctcctgttct gcactcgttg ctggatacag
     7261 atgcttataa gttgcatatg cagcaagccg tgtttcatca ctattacgat gtgcatgtcg
     7321 cggcggagtt tcgttgccga ggtgacgatc tgctgggtat ttatgccgat gctattcgtg
     7381 aacagattca ggcgatgcag cacctgcgcc tgcaggatga tgaatatcag tggctctctg
     7441 ccctgccttt ctttaaggcc gactatctta actggttacg cgaattccgc tttaacccgg
     7501 aacaagtcac cgtgtccaac gataatggca agctggatat tcgcttaagc ggtccgtggc
     7561 gtgaagtcat cctctgggaa gttcctttgc tggcggttat cagtgaaatg gtacatcgct
     7621 atcgctcacc gcaggccgac gttgcgcaag ccctcgacac gctggaaagc aaattagtcg
     7681 acttctcggc gttaaccgcc ggtcttgata tgtcgcgctt ccatctgatg gattttggca
     7741 cccgtcgccg tttttctcgc gaagtacaag aaaccatcgt taagcgtctg caacaggaat
     7801 cctggttcgt gggcaccagc aactacgatc tggcgcgtcg gctttccctc acgccgatgg
     7861 gaacacaggc gcacgaatgg ttccaggcac atcagcaaat cagcccggac ctagccaaca
     7921 gccagcgagc tgcacttgct gcctggctgg aagaatatcc cgaccaactt ggcattgcat
     7981 taaccgactg catcactatg gatgctttcc tgcgtgattt cggtgtcgag ttcgccagtc
     8041 ggtatcaggg cctgcgtcat gactctggcg acccggttga atggggtgaa aaagccattg
     8101 cacattataa aaagctggga attgatccac agagtaaaac gctggttttc tctgacaatc
     8161 tggatttacg caaagcggtt gagctatacc gccacttctc ttcccgcgtg caattaagtt
     8221 ttggtattgg gactcgcctg acctgcgata tcccccaggt aaaacccctg aatattgtga
     8281 ttaagttggt agagtgtaac ggtaaaccgg tggcgaaact ttctgacagc cctggcaaaa
     8341 ctatctgcca tgataaagcg tttgttcggg cgctgcgcaa agcgttcgac cttccgcata
     8401 ttaaaaaagc cagttaatat catcagggag ctaatcggct cccttttttt acctttaatt
     8461 ccgaaatctt tcgctgcatt tgcgaattct gcttgtctga ttgcagatgc caggtaacat
     8521 aggtatcccc ccatttacgg gtggacaagt gtttattttt tccgactatt aacagagaga
     8581 atattatgag cgttgtgcct gtagccgacg tactccaggg ccgcgtagcc gttgacagcg
     8641 aagtcaccgt acgcggatgg gtacgtaccc gccgagattc aaaagctggc atctccttcc
     8701 tcgccgttta tgacggttcc tgctttgatc ctgtacaggc tgtcatcaat aattctctgc
     8761 ccaattacaa tgaagacgtc ctgcgtctga ccaccggctg ctcggtcatt gtgacgggta
     8821 aagtcgtggc gtcgccgggc caggggcaac aatttgaaat tcaggccagc aaagttgaag
     8881 ttgctggttg ggttgaagat ccagacactt acccgatggc ggcaaaacgc cacagcattg
     8941 agtatctgcg tgaagtcgct cacctgcgtc cgcgcacaaa cctgattggt gccgtcgcgc
     9001 gcgttcgcca tacgctggcg caggcgctgc atcgcttctt taacgagcag ggattcttct
     9061 gggtttcaac gccactgatt accgcatctg ataccgaagg tgcaggcgaa atgttccgcg
     9121 tttctacgct ggatctggaa aacctgccgc gtaacgatca gggcaaagtg gatttcgaca
     9181 aagacttctt tggtaaagag tctttcctga ccgtatctgg ccagttgaac ggcgaaacct
     9241 acgcttgcgc attatccaaa atttatacct tcggcccgac tttccgtgct gaaaactcca
     9301 acaccagccg tcacctggcg gaattctgga tgctggagcc ggaagtggcg tttgctaacc
     9361 tgaacgatat cgcgggtctg gctgaagcca tgctgaaata tgtcttcaaa gcggttctcg
     9421 aagaacgcgc agacgacatg aaattcttcg ctgaacgcgt agataaagat gccgtttcac
     9481 gtctggaacg cttcattgag gccgatttcg cgcaggtgga ttacaccgac gcagtgacca
     9541 ttctcgaaaa ctgcggcagg aagtttgaaa acccggttta ctggggcgtc gatctctctt
     9601 ctgagcatga gcgttatctg gcggaagaac actttaaagc accggtagtg gttaaaaact
     9661 atccgaaaga tattaaagcg ttctatatgc gcctcaacga agacggtaaa accgttgcgg
     9721 ctatggacgt tctggctccg ggcatcggtg agatcattgg aggctcccag cgtgaagaac
     9781 gtctggacgt tctggacgag cgtatgctgg aaatgggcct gaataaagaa gattactggt
     9841 ggtatcgcga tctgcgtcgc tacggtactg ttccgcattc aggtttcggt cttggttttg
     9901 aacgcctgat tgcttacgta actggcgtgc aaaacgtgcg tgatgtgatt ccgttcccac
     9961 gtactccgcg caacgccagc ttctgatttt cttcctcaaa gagccagcgc ctgctggctc
    10021 ttttttatcc tttctgtcaa gttatctgtt tgttaagtca agcaatctat ttgcaacccc
    10081 gccataacga tcatcctgtt acggaatatt acattgcaac atttacgcac aaaaaataac
    10141 ccgtattctt attgcggatt agttttttct tagctgatag cacaattttc atactatttt
    10201 ttggcattct ggatgtctga aagaggattt tgtgccaggt cgataaagtt tccatcagaa
    10261 acaaaatttc cg
//
LOCUS       DAZPEB010000149        10117 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZPEB010000149 DAZPEB010000000
VERSION     DAZPEB010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10117
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.152"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            205..357
                     /gene="hokA"
                     /locus_tag="ACHURJ_003165"
     CDS             205..357
                     /gene="hokA"
                     /locus_tag="ACHURJ_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFR1282006.1"
                     /translation="MPQKYGLLSLIVICFTLLFFTWMVRDSLCELHIKQGRYELAAFL
                     ACNLKE"
     gene            complement(346..405)
                     /locus_tag="ACHURJ_003166"
     CDS             complement(346..405)
                     /locus_tag="ACHURJ_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282007.1"
                     /translation="MRNAVRKAGIISRRRLLLF"
     gene            complement(545..757)
                     /gene="cspA"
                     /locus_tag="ACHURJ_003167"
     CDS             complement(545..757)
                     /gene="cspA"
                     /locus_tag="ACHURJ_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFR1282008.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(1038..1328)
                     /locus_tag="ACHURJ_003168"
     CDS             complement(1038..1328)
                     /locus_tag="ACHURJ_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFR1282009.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGISVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1761..2471
                     /locus_tag="ACHURJ_003169"
     CDS             1761..2471
                     /locus_tag="ACHURJ_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFR1282010.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQTDDLKPVFDQAFTKVVTTP
                     ADALQPLIPVAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2521..3495)
                     /gene="ghrB"
                     /locus_tag="ACHURJ_003170"
     CDS             complement(2521..3495)
                     /gene="ghrB"
                     /locus_tag="ACHURJ_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFR1282011.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKPSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3599..4258)
                     /locus_tag="ACHURJ_003171"
     CDS             complement(3599..4258)
                     /locus_tag="ACHURJ_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFR1282012.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLDSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            4411..6744
                     /gene="bisC"
                     /locus_tag="ACHURJ_003172"
     CDS             4411..6744
                     /gene="bisC"
                     /locus_tag="ACHURJ_003172"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFR1282013.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVHVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAGICGVHAAKIRELAALFHQNTTMLMAGWGMQRQQLGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRCSAVLSSMQGSLPGGSDAVDK
                     IPVARIVEALENPGCAYQHNGMNRLFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSELQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPQAHPLKTASGKIEIFSQRIADYAYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDALARGIQD
                     GDTVRLWNARGQILAGVVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6713..7153)
                     /locus_tag="ACHURJ_003173"
     CDS             complement(6713..7153)
                     /locus_tag="ACHURJ_003173"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFR1282014.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDSKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRHPHLML
                     EVYQKNQPAINFYQAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7150..7713)
                     /gene="tag"
                     /locus_tag="ACHURJ_003174"
     CDS             complement(7150..7713)
                     /gene="tag"
                     /locus_tag="ACHURJ_003174"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFR1282015.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7871..8569
                     /locus_tag="ACHURJ_003175"
     CDS             7871..8569
                     /locus_tag="ACHURJ_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFR1282016.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8797..9999
                     /locus_tag="ACHURJ_003176"
     CDS             8797..9999
                     /locus_tag="ACHURJ_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1282017.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSDNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHI"
BASE COUNT         2427 a         2434 c         2592 g         2664 t
ORIGIN      
        1 ttttttagga ttatatcaag gagaagaaac aaacttatta agctagaata gctacgggtg
       61 cttgagactg tttgtctcag gtattcaccg aaaggcagac agagaaaagc cccacctgac
      121 tataaatcaa agtgaggcta ccctatgcct gaacaccata aggttagcct cttactcgtt
      181 ggaaatcaac acagggggct gggaatgccg caaaaatacg gattactttc attaatagtg
      241 atttgtttca cgcttttatt tttcacctgg atggtaagag attcactgtg tgaattgcat
      301 attaaacagg ggcgttatga gctggcggca tttttagcct gcaatttaaa agagtaagag
      361 tcttcggcgg gaaattattc ccgcctttct tacggcgttg cgcattctca ttgcacccac
      421 atttattctt cacaaaaata ataatagatt ttactacgcg atcgattatt tatttcctga
      481 aaaaaaataa aaaaaatccc cgccaaatgg cagggatctt agattctgtg cttttaagca
      541 gagattacag gctggttacg ttaccagctg ccgggccttt agcgccgctt tcgatggtga
      601 aggacacttt ctgaccttcg tccagagatt tgtaaccatc gttctggata gcagagaagt
      661 gtacgaacac atctttagag ccatcgtcag gagtgatgaa gccgaagcct ttgtcagcgt
      721 tgaaccattt tacgatacca gtcattttac cggacatagt gtattacctt taataattaa
      781 gtgtgccttt cggcgatatg gcgtgcttta cagattttga agcgttaaag gaatgtgcac
      841 tacgaggggt atcaacgata actcttgaag ggacttgcct tactacactg ctttaatggt
      901 ctgtacgtca aaccgttgat gtgcattaag ccacgcattg gcgggtgatg caacaattat
      961 ttttcatatt tatgattaat cggttggaaa acggtcctgt catcaggacc gtaaacagca
     1021 ataaagtgga taaaagtcta ttccatcaac tgcttactta atgccgggtt ggcttgaatc
     1081 aaacgcatca attttagttc ggcacttgaa ggcttcacgc gtctggattc ccattccttt
     1141 accatggcga ctgatatgcc caaaacccgg gcgaaatcat cgatttttaa ccctgtccct
     1201 tttcgtaact gctcaatttc ggtacaggac gttgttctgt gcgtcagggt aattttctgc
     1261 gtttcatctt taaaaacaat ctgttccagg ctgctcaaca gctcatgcat tggatcttta
     1321 tattccattg aaaactcctc aaatcacact gcgggatcgt gaattgcatc gaagctcatt
     1381 aagaatagtc gggaaaagca gacacagcgg ggtgaagtca gggcatgctc tggctatgat
     1441 tcgattcagc cagtcattta gcgtgactta tagccaccta acgatctgat aaatgccgat
     1501 atatctctta ccactcctgt tttgtaaaga tttcgaaaat ctaaagttct gcgaaggcgt
     1561 aaatctcggg ctacaaaaaa gcaacggcaa tatcattttt taacaatgaa acaacaaatt
     1621 aaccttgttt cattttctcc atgattttac caggaagatt acgtagaaag ggtatcctgc
     1681 aagcctgtcc tggactatcc ttgtcacgtc agacacgcgt gtgtcgttgt gcgctttttt
     1741 tgggtgaaag gagtaagaaa atggcgacag gaaagtcctg ctctcgctgg tttgcgcctc
     1801 ttgcggcgtt attaatggta gttagcctga gtgggtgttt tgataaagaa ggcgatcagc
     1861 gtaaagcgtt tatcgacttc ctgcagaata cagtgatgcg tagcggtgaa cgtctaccaa
     1921 ccctgactgc cgatcagaaa aaacagtttg gtccttttgt ctctgattac gcgattctgt
     1981 atggttattc tcagcaggtg aatcaggcga tggattccgg tctgcgcccg gtcgtagaca
     2041 gcgttaacgc aattcgcgta ccgcaggatt acgttacgca aagcggtccg ctgcgtgaaa
     2101 tgaacggttc gctgggtgtg ctggcgcaac aactgcaaaa tgcgaagttg caggccgatg
     2161 ctgcgcactc tgcgctaaaa cagaccgatg atttgaaacc ggtctttgat caggcattca
     2221 ccaaagtggt aacgacgcca gctgatgcat tgcaaccgtt gattccggtt gcacaaactt
     2281 tcacgcaaca actggtgatg gttggagact acattgctca acaaggtact caggtgagct
     2341 ttgtagcaaa tggtatccag ttcccgactt cgcagcaggc aagtgaatat aacaaactga
     2401 ttgcgccgtt accggcacaa catcaggcgt ttaatcaggc ctggactaca gcagttactg
     2461 caacccaata aagagtaaaa gcccgagcga ttatcgggct ttacttcacg cagtcgcggc
     2521 ttagtccgcg acgtgcggat tcacacagtt cttctcaacc tttccttgta acgcatcaat
     2581 caaattatcc acggcacagg cggccatgcc ataacgcgtc tcatgggtgg cagatccaat
     2641 atgcggtact gcgacgacgt tggccattga gagcaacggc gaatctacgg acagtggctc
     2701 ttgttcgaag acatccagcc cggcggcgtg aatttcgcct ttctgcaatg ctgcgatcag
     2761 tgcattttcg tcaaccaccg ggccacgtcc ggcattaatg aaaatggcgg agggtttcat
     2821 tttggcgaat tgttctgcgc caaacagatg atgcgtctca tcagttaacg gcaggatcag
     2881 gcaaacgaaa tctgactctt gtaacagagt atccaaatcg cagtagcggg cgttgaagcg
     2941 ttcttctgct tctttatggt ggcggcgcgc gttatagagg atgggcatgt tgaagccaaa
     3001 gtgcgcacgt tgtgccagcg ccatgccgat ccgtcccatc ccgacaatgc ccagtgtttt
     3061 atggtgaacg tcagtgccgt accagtccgg gcctatgctc gcggtccatt cgcctgcttt
     3121 tacccgttct gctacctcca caacccgacg agcggtagac aacaccagcg ccatcagcgt
     3181 atcggcgacg gtttctgtta atacggttgg cgtgtgcatc agcagaattt ttcgggcggt
     3241 aagcgcatcg acatcaaaat tgtcatagcc gacggagatc gttgatgtgg cacgcagttt
     3301 cggcattttt tccagcaatg cggcatctac attctcgttt gaacccagta aaccttcagc
     3361 ttcggcaaaa attgctgcat tttgttcgac ggtttgtggg ctgaggtttg ccacctggtg
     3421 aacggtgaaa tgctcttgca ggcgttgcag taaatcatca ggtaaggctt tgtagaggat
     3481 aacggacggc ttcatgcttc tctccattca ctgataaggc gctcacctta gcctgaaata
     3541 tgatgaagga aaactgaaga atgccaggtg atttctgcat cacctggcat gaaagggatt
     3601 acagcgggct taaggtaatt tctacacggc ggttttgcgc cttaccttct gcggtgctgt
     3661 tgctggcgat tgggttagcc gggccaaggc cctgagtacg gatgcggctg gcgtccacgc
     3721 cctgggtgat caacgcgctg gcaacggaat ccgcacgctg ctgggagaga cgcatgttca
     3781 ggtcgtgacc ccccgtgctg tcggtataac caatcacgtt aaccgccgtt ttcggatact
     3841 ctttcagcac cattgccacg ccggtcaggg tgttagcgcc tgccggtttc aggggcgcgc
     3901 tgctgctgtc gaaggtcaca ttgttcggca tattgaggat aatgttatcc ccgctgcggg
     3961 ttacgctaac accagtgccg cgcattttgt cacgcagctt cgcttcctgc acatccatgt
     4021 aataacccac gccgccgccc agagctgcgc ctgctgctgc gccaatcagc gcgcctttac
     4081 cgcgatcttt cttcgaagaa gagagcgcac caatacccgc gcccacgaga gagtccagac
     4141 ctgcgccgat agcagatttg cctgcttcgc gttcgccggt gtaagggtta gttgtgcagc
     4201 cagataccgc cagagcgcca ctcactacgg cggcaataag ataaacacgt ttcttcattg
     4261 ttaatcctta ataacctttt tattctttgc cacgggttcc gtggcgggag attatgccgc
     4321 gtgaacatga agattattcc tgggaatact cggaaatttg taagtaatat ttaactgctc
     4381 aatacatcta acctttcagg agtcttcggt ttggccaact catcctcacg atattccgtt
     4441 ctgactgccg cccactgggg gcccatgctg gttgaaaccg acggcgaaac cgtgtttagc
     4501 tcgcgtggcg cgttagccac aggaatggaa aactccttgc agagcgcggt tcgcgaccag
     4561 gttcacagca atacgcgggt acgatttcca atggtgcgca aaggctttct cgcgtcaccg
     4621 gaaaacccgc aaggcattcg tgggcaggat gaatttgttc acgtgagttg ggatgaggcg
     4681 ctggatctta ttcaccaaca acataaacgc attcgtgagg cttatggtcc ggcatcgatt
     4741 tttgctggtt cctacggctg gcgttcaaac ggcgtgctgc ataaggcctc gacattatta
     4801 caacgctata tggcgctggc aggcggttat accgggcatc tgggggatta ttcgaccggt
     4861 gcggcacagg cgatcatgcc gtatgtcgtg ggtggcagcg aagtttatca acagcaaacc
     4921 agttggccgc tggtgctgga acatagcgat gtcgtggtgt tgtggagtgc taacccactc
     4981 aatacgctga aaattgcgtg gaatgcatct gatgagcagg ggctttctta cttttccgca
     5041 ctgcgtgaca gcgggaaaaa gctgatctgt attgatccaa tgcgatcgga aaccgtcgat
     5101 ttctttggcg ataaaatgga gtgggtggca ccgcacatgg gcaccgatgt tgcgctgatg
     5161 ctggggatcg cccatacgct ggtggaaaat ggttggcacg acgaagcgtt tctggcgcgt
     5221 tgcaccacag gttatgccgt cttcgcctct tatttgctgg gcgagagtga cggaatagcc
     5281 aaaaccgccg aatgggcggc agggatttgt ggggttcacg cagcgaaaat tcgcgagttg
     5341 gcggctcttt tccaccaaaa cacgaccatg ctgatggctg gctggggaat gcagcgccaa
     5401 cagcttggtg agcaaaaaca ctggatgatc gtcacgctgg cggcaatgtt ggggcaaatc
     5461 ggcacacccg gcggcggttt tggtctttct tatcattttg ccaatggtgg taatcccacg
     5521 cggtgttctg cggtgctctc ttccatgcag ggtagcttgc cgggtggcag cgatgcggta
     5581 gataaaatcc ctgttgcccg tattgttgaa gcactggaaa accctggctg cgcataccaa
     5641 cacaatggca tgaatcgcct cttcccggat attcgcttta tctggtgggc gggtggcgcc
     5701 aacttcaccc atcatcagga cactaatcgc ctgatacgag cttggcaaaa accggagctg
     5761 gtagttatct ccgaatgctt ctggacggcg gcggcaaaac acgcggatat cgttctgcct
     5821 gcgactacct cttttgagcg taatgatctc accatgaccg gcgattacag taatcagcat
     5881 ctggtgccga tgaagcaagt ggtgccgcca cgctatgaag cgcgtaatga ttttgatgtt
     5941 tttgccgagt taagtgaacg ctgggagaag ggcggttatg cacgttttac ggaaggaaaa
     6001 agtgagttgc aatggctgga aacgttttat aacgttgccc gacagcgcgg ggcaagccag
     6061 caggttgaat tgccgccatt tgctgagttc tggcaagcca atcagttaat tgagatgccg
     6121 gaaaacccgg acagcgagcg gtttattcgc ttcgctgatt tttgccgcga tccgcaggcg
     6181 catccattaa aaaccgccag cggtaagatt gaaatcttct cgcagcgtat tgccgattac
     6241 gcttatccgg attgcccggg gcatccaatg tggctggagc cggacgaatg gcagggcaat
     6301 gccgaaccgg aacagttaca ggtactttct gctcatccgg cgcatcgtct gcacagccag
     6361 ctgaattaca gttctctgcg cgaattgtat gcggtggcaa atcgtgagcc tgtcaccatt
     6421 catcctgacg atgccctggc gcgcggcatt caagatggcg atactgttcg gctgtggaac
     6481 gcacgcgggc aaattcttgc cggagtggtc attagcgagg gaattaaacc tggcgtgatt
     6541 tgtattcacg aaggggcatg gccggatttg gatttaaccg ctgacggtat ttgtaaaaac
     6601 ggcgcagtca acgtgctgac caaagatctc cccagctcgc ggctggggaa tggctgtgcg
     6661 ggtaatacgg cgctggcatg gctggaaaaa tacaacggtc cggaactgac acttacagcg
     6721 tttgaaccac cggccagctc ataatccagg tgggtagttg ggtttcatcc tgccatgcgc
     6781 aatcgacaat gtgaaaaccc tgcgcttggt aaaaatttat cgccggttga tttttttgat
     6841 aaacctccag catcaggtgg ggatggcgct gctgcacata ttgcatcagc gccttaccaa
     6901 taccgcgcct gacggccttc ggtgcgacaa acatcgctgc cagaaatcgg ccttccataa
     6961 tgctgacaaa accgagaagc ttactgtctt cttcccagac ccagttttgc gcattggcaa
     7021 gatacgcatc ccgcaccagc ggaatgcaat cacgccagta attcgctttt ataaagggat
     7081 gtccccaggt tgtactttcc agccacagtt cgaggatcgc ggggagttct gaacgttgcg
     7141 cttcccgaat catggtttat ttcccggata gcaacagcag ccaaccacat gatcattcac
     7201 cagcccacat gcctgcataa aggagtaaca gattgtggta ccgacaaact taaaaccacg
     7261 ttttttcagt gctttagata gggcgtcgga ggcgggcgta gatgtgggga tttcgctcaa
     7321 cgttgtggct tgtgtcacct gtggctgatg atttacaaac gaccagacaa agtcggcaaa
     7381 cggttcgccg ttctgttcca tttgcaggta cgcccgcgca ttaccaataa ttgcctgaat
     7441 tttcccgcga tggcggataa tcccggcgtc ctgtaccagt ctttcgacat cctcttcctg
     7501 cattgctgcg accttcaccg gatcgaactg atgaaagcag gcgcgatagt tttcgcgttt
     7561 tttgaggacg gtgatccacg ataacccagc ctgctgccct tcaaggcaga tcatttcgaa
     7621 cagtttttta ctgtcagttt caggcacgcc ccactcatta tcatggtagg caatataaag
     7681 cgggtcctga ctcacccagc cgcaacgttc catactttcc ctcgctatgt acccgatcgt
     7741 aaaaatttca gccgcttttc ttgacgcatc ggtgaaaaag tcaatattta atacagggct
     7801 actatgcccg atatccttca tacaatgaca ataatattgc cgaattcggc tcttccctgg
     7861 agtcgtgtta atgatcataa aaaaaagcgg tggtcgctgg cagctaagcc tgctggcgag
     7921 cgtggtaatc agcgcctttt ttctcaacac agcttacgcc tggcaacaag aatatatcgt
     7981 tgatacgcaa cccggacatt ccacagagcg ttacacctgg gatagtgatc atcaacctga
     8041 ttacaacgat attttgtcgc aacgtattca aagtagccaa agggcgctgg gactggaagt
     8101 caatctggcg gaagaaactc ctgtggatgt gaccagcagt atgagtatgg gctggaattt
     8161 tcctttatat gaacaggtta caaccggccc ggtcgcggca ttacattacg atggcacaac
     8221 cacctcgatg tataacgagt ttggcgacag tactaccacg ctgaccgatc cgttatggca
     8281 tgccagcgtg agtagcttag gctggcgtgt tgactcccgg cttggcgatc tccgaccctg
     8341 ggcgcaaatc agctataacc agcaatttgg cgagaatatc tggaaggcgc aatcaggcct
     8401 gagccggatg acggcgacaa accagaacgg caactggctg gatgtcaccg taggcgctga
     8461 tatgttgctc aatcaaaata ttgccgccta tgccgcgcta actcaggcag aaaataccac
     8521 taataatagc gactatctgt atacgatggg ggttagcgcc agattttaac gtaacagtca
     8581 caattgaaac cattgaataa caatagttgt ggtgatagtg ggtgctaact tacaaataat
     8641 aaatttggtg aataattgtc gcgtcattca ttcctgaact aaggcatttc attccgttct
     8701 gatggcattt catgccgttt ttccccaggc ataaaatgca cttcgttatg gttgtcggca
     8761 gagatttttc ctttttatta ctgcaggaat actgccatga caccttcaaa ttatcagcgt
     8821 acccgctggc tgacactcat cggtactatc attacccagt ttgcgctggg gtcggtttat
     8881 acctggagcc tgtttaatgg cgcgctttct gccaagctgg atgcgccggt aagccaggtc
     8941 gctttctctt tcggcttgtt aagtctgggg ctggcaattt catcttctgt tgcggggaaa
     9001 ttacaggaac gttttggcgt taaacgcgtc actatggctt ccggcatttt gctaggatta
     9061 ggcttcttcc tgacagcgca ttctgacaac ctgatgatgc tgtggttaag cgccggtgtg
     9121 ctggtcggtc tggcggatgg cgcgggttac ctgctgacgc tctctaactg cgtgaagtgg
     9181 ttcccggagc gtaaaggtct gatctccgcg ttcgctatcg gttcttatgg tcttggtagc
     9241 ctgggtttca aatttatcga cacgcagctg ctggaaacgg tgggtctgga aaaaacattt
     9301 gtgatttggg gagcgattgc gctggtgatg attgttttcg gcgcaacgtt aatgaaagac
     9361 gcaccaaaac aggaagtgaa aaccagcaat ggtgtggtgg agaaagatta cacgctggca
     9421 gagtcgatgc gtaaaccgca gtactggatg ttagcggtaa tgttcctgac tgcctgcatg
     9481 agcggcctgt acgtgattgg ggtagcgaaa gatatcgccc aaagtctggc acaccttgat
     9541 gtggtttccg cagccaatgc agtaacggtt atttccatcg ctaacctttc aggtcgtctg
     9601 gtgctgggca ttctgtctga caaaattgcc cgtatccgtg ttatcaccat tggtcaggtg
     9661 atttcgctgg tgggtatggc ggccctgctg tttgcaccat taaatgcagt gacgttcttt
     9721 gcagcgattg cctgcgtggc atttaacttt ggcggcacta ttaccgtctt tccatcactg
     9781 gtcagtgagt tcttcggcct caataacctg gcgaaaaact acggtgtgat ttacctcggt
     9841 ttcggtatcg gtagcatttt tggttcgatt atcgcctcac tgtttggcgg tttctatgtg
     9901 accttctacg tgattttcgc cctgctgatt ctgtcattgg cgctttctac gacgattcgt
     9961 cagccagagc agaaaatgtt gcgtgaggcg catatttaga aatatgttgc tactgaaata
    10021 aatacgagga gggagacttc aaagtctccc ttttttatgg acgcatatta aaagaacaat
    10081 aatattcata ccacaaatat attttattta tatatta
//
LOCUS       DAZPEB010000150        10062 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZPEB010000150 DAZPEB010000000
VERSION     DAZPEB010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10062
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.153"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            6..81
                     /locus_tag="ACHURJ_003177"
     tRNA            6..81
                     /locus_tag="ACHURJ_003177"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="ACHURJ_003178"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="ACHURJ_003178"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(468..1226)
                     /locus_tag="ACHURJ_003179"
     CDS             complement(468..1226)
                     /locus_tag="ACHURJ_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFR1282018.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFTHPKSERTRAFLSQVIH"
     gene            complement(1234..2337)
                     /locus_tag="ACHURJ_003180"
     CDS             complement(1234..2337)
                     /locus_tag="ACHURJ_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFR1282019.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     VLLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2347..3517)
                     /locus_tag="ACHURJ_003181"
                     /pseudo
     CDS             complement(2347..3517)
                     /locus_tag="ACHURJ_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(3585..4634)
                     /locus_tag="ACHURJ_003182"
     CDS             complement(3585..4634)
                     /locus_tag="ACHURJ_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR1282020.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LLGFSYADADGKFSGIDVDVDVDVDVDVCRSVAAAVFGDDTKVKYTPLTAKERFTALQ
                     SGEVDLLSRNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQ
                     AGTDTELNVADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLS
                     NPAEWIVLPEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAAN
                     PATPDMAHLLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQN
                     NLWNNGGIQYAPPVR"
     gene            complement(5065..5286)
                     /locus_tag="ACHURJ_003183"
     CDS             complement(5065..5286)
                     /locus_tag="ACHURJ_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282021.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGCLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            complement(5539..8643)
                     /gene="acrF"
                     /locus_tag="ACHURJ_003184"
     CDS             complement(5539..8643)
                     /gene="acrF"
                     /locus_tag="ACHURJ_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HFR1282022.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTNDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERNGDENSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALSV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFLPVFFVVIRR
                     CFKG"
     gene            complement(8655..9812)
                     /gene="acrE"
                     /locus_tag="ACHURJ_003185"
     CDS             complement(8655..9812)
                     /gene="acrE"
                     /locus_tag="ACHURJ_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFR1282023.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYNSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     GTVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMYVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
BASE COUNT         2685 a         2587 c         2408 g         2382 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcaaattaga aaagaatttg
      121 tctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt ggggtcttcc catgcgagag tagggaactg ccagacatca
      241 aataaaacaa aaggcccagt cttccgactg agccttttgt tttatctgtt gtttgtcggt
      301 gaacactctc tcgagtagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg
      361 gcccggaggg tggcgggcag gacgcccgcc ataaactgcc agacatcaaa tcaagcaaaa
      421 aggccatccg aaaggatggc ctttttgctt ttcgaactaa cattcaatta atggattacc
      481 tgcgataaaa atgccctcgt acgctctgat ttaggatgcg taaaaaattc atcaggtgca
      541 gcttgttcca ctatctcccc acgatccata aaaattaccc ggtcagcgac ggtacgtgca
      601 aaccccatct catgtgttac acacaacatt gtcatacccg actgcgccag cccaatcatc
      661 gtatccagca cctctttcac catctccgga tcgagcgccg acgttggttc atcaaacaac
      721 ataattttcg gcttcataca cagcgaacgc gcaatggcaa cgcgttgctg ctgaccacct
      781 gaaatctgtc cgggaaactt atgcgcatgt tcggcaattc tcacccgctc aaggtaatgc
      841 atcgccagag cttcagcctc tttcttaggc atcttgcgta cccaaatcgg tgccagggta
      901 cagttctgta aaacggtcag atgagggaag agattgaaat gctgaaatac cattcccact
      961 tcttgcctga cgcgctcaat attgcggata tcttcattaa gttcgatgcc atctacgaca
     1021 atccgtccct gttgatgttc ttccagatga ttaatacaac gaatggttgt cgatttaccg
     1081 gaaccagaag ggccacacag aacgatccgt tctcccggtt gcacggttaa atttatattt
     1141 ttcaaaacat ggaattgtcc ataccattta tggacgtttt ccagcgtaat catcgcgtta
     1201 gcaggttgca gtaaaatttg gctcatagtg tcctcaatgc ggtgtacgcc cggtgttaaa
     1261 acgtttttcc agatgttggc tataacgtga catgctgaaa caaaagatcc agtagatcag
     1321 cgcggcgaaa acataccctt ccgtcgacat acccaaccag gcgggatcaa cggttgcctg
     1381 ctgaacgcta ctgaaaagat cgaacaagcc gatgatgatc accaggctgg tatctttgaa
     1441 gagtgcgatg atggtattta ccagcccagg aattaccagc ttcaacgcct gtggcagaat
     1501 aaccagcccc tgagttttcc agtaccccaa cgccagcgac tctgccgctt catattgccc
     1561 tttaggtagg gcctgtaatc cgcctcgcac gacttccgca acatatgctg actgaaacag
     1621 gatcacgcca accagcgcgc ggatcagttt gtcgatactg gtgccttctg ccataaacaa
     1681 cggcagcatg accgaagaca taaacaatac ggtaatcagc ggtacgccgc gccagaactc
     1741 gataaaaatg accgataaga tacgcacaat cggcatatgg gagcggcgac ctaacgccag
     1801 taagatcccc cacggtagcg cccccgcaat cccaacggat gcaataatta aagttagcgt
     1861 cagcccgccc cattgccggg tttcaacccg ctcaagacca aaaaaaccgc catacatcag
     1921 ccaccagaca atcagtgggt aaatcaccgc ccagaccgca atatagcgac cgcgatgcgg
     1981 gagtattttc cagaacattg gtgcgattga cacaaggcca atcagtaata ccaggttaat
     2041 tcgccagcgt tggtcgtgtg ggtaaagtcc atacataaac tgaccaaatc gttcgtggat
     2101 gaagacccaa caggcaccgg cttttgtgca gtcggcacgc gttgagccaa cccagttagc
     2161 ctgcaaaaat gcccagttca gcaacggtgg gatcagttcc cacatcaacc atatgcagcc
     2221 aatagtcagc aggctattgc tccagctgga gaacagattt tttcgcaccc acaccatcgc
     2281 gcggcttgag ttatggctcg ctgggcgcga gggctgagac agcaatactt ttgtcatcat
     2341 gactccttag cgttcaacga tcgcgatgcg gcggttatag atattcatca gcagcgagat
     2401 agtcaggctg ataatcagat agaccgacat ggtcatggct atcgtctcga tggcttgccc
     2461 cgtctggttc agcacggtgc cagcaaacag cgaaaccata tcgggatagc caatagcggc
     2521 ggcaagagag gagtttttga cgatgttgag atactggctg gttaacggcg gaataatcac
     2581 tcgcagtgcc tggggaataa tgacctggcg tagcgtaacc ccagtgaccg agccgcttca
     2641 tgttgaccat aaggcactgc ctggatccca gcgcggataa tctcggcgat aaatgcagag
     2701 gtataaaccg aaagtgccag cgttaaggct gccagttcag gaattaaaac cattccgccg
     2761 cggaagttaa agcctcgtag ggctgggaca tcccagtgta gtgctgcgcc aaaaagccat
     2821 tgcgccagta aaggcaaacc gatgatcaac actgcggcga acggccaggt gcggcgcagt
     2881 tgtccggtct ttatctggta tgttttatta aagcggaata gcccaacaga aaggactata
     2941 gccataacaa cagccaggat aaacgcaata aatccgtctc ccagctgcgg tgacggaata
     3001 taaagcccac gattgctcaa aaaggccaga tcaaacgcgc tcacagcctg gcgcgtggtc
     3061 cgggcaaatt gcgcaacacg gcaaagtacc agaagaagat ttgcagcagc gggggaatat
     3121 tacggaagat ctcaatataa attgtggata gctttcgtag cagtcagtta tccgaaagtc
     3181 tcgtcagacc gataaagaag cctaaaacag aagcgaacac aatacacaat gcagaaacca
     3241 gtagcgtatt gagtaagccg acaataaaaa cgcgtccgta ggtgtcgccc tgctggtaat
     3301 cgatcaaatg ctggacaata ccgaagccag cgccgcgatc cagaaaggca aaacctgaag
     3361 taatgccacg attactgaga ttcgttacag tgttgtgaaa taaccaacca acaattccga
     3421 caacagcaac aacggcaagg atctggaata accaggcacg aaccgtaggg ttggcaaagg
     3481 agagtgagcc tttaacggtt gagcggcgat gagacataga aacctcggaa agcataacgc
     3541 tgaagagagc gccgccgtag cgacgctcgt tacctcacac cgacttaacg cacgggcggc
     3601 gcgtactgaa taccgccgtt attccagaga ttattttgcc cacgtttaat tttcagcggg
     3661 ctttctgaac ctacgttacg ctcgaaaatt tccgagtagt tacccacctg tttgatgatg
     3721 ttataggccc atttattatc caacttcaga tccttgccgt aatcgccttc tttacccagc
     3781 agatgtgcca tatcaggcgt tgctggatta gccgcttttt catcgacgtt ctgggaattg
     3841 atgcccatct cttcagcatt cagcatggcg aaaagcgtcc agcgtacaat cgagaaccat
     3901 tcatcatcgc cacgacgaac taccggccca agcggttctt tagagataac ctccggtaag
     3961 acaatccatt cagctgggtt gcttaatttg atgcgcaggg catacagttg cgactgatcc
     4021 gaggccagcg tatcgcagcg accagattcc agtgccttcg ctgattcgtc agagcgatcg
     4081 aaagtcaccg gtgtgtactt catattgttt gccttgaaat agtcggcaac gttgagttcg
     4141 gtatcagtac ccgcctgaat acagacggta gcgccatcca gttctttcgc gctttttagc
     4201 cccgctttat cgtgcgtcag gaagccaatg ccgtcgtaat aagtgacgcc agtaaatgcc
     4261 attcccatcc cggcgtcgcg agatgaagtc caggtcgtat tacgggagag caaatccact
     4321 tcccctgact gtaaagccgt gaagcgttct tttgctgtga gtggggtata tttcactttc
     4381 gtgtcgtcac caaatacagc agcggcaaca ctacgacaaa catcaacatc aacatcaaca
     4441 tcaacatcaa catcaatacc tgaaaactta ccgtctgcat cggcataaga gaacccaagt
     4501 aatccatcac tgatcccgca ttgcacaaaa ccttttttct gaacggcatc aagcgtcgcg
     4561 ccagcatgcg cctgatttgc aacggcaagc agcacgctgg cggcagccag tgtggctatc
     4621 atcatctttt tcataatgct tcctgtgagg cgaaaattat cgttatagga cgtttgggaa
     4681 acgtctttta cctgtctgtg gccatcctct tataagcaaa agtagtgcca gcttttgcgc
     4741 acgatttcgc gtcgcaataa gctgagtgta gacaggaaat aataaaaaca gcgccccgca
     4801 atggtgcgaa aatgaaacat gcgccacatt ttggcgcaaa atatttacgc gtgttttact
     4861 aagcagaata atgattgtta gcgtgagtat atttctggtg ggaagtgtga aagttaaatt
     4921 gatgcgttat ttcgaaagca gcgaaataac gcgatataac atgctaaatc agcagagaat
     4981 tagccagcag aatatagcaa tattttatct tatgtaaaga tactgtatcg ttaaacagag
     5041 cgcgaaactc cgcgccctgt tcagttattt cgacagagcg atgatcccca tcgtcaggcc
     5101 agcaacagcc gctgccccgc cggcaatagc cccagccgtt tcccagttat cctgagtcgt
     5161 gcctttcata caggtattgg tgtgaaccag acaaccctga tcgtcataaa ccggaacgca
     5221 gggcgaatcg tgagcacagc ccgccagcaa tgcggtgagc aatgccacag gaattaatct
     5281 tttcatggtg ttacctcagt aataaacgct aacgcttaat aagttaactc tcttattaac
     5341 gagctgaatg ccattttatc accggaggtt ataaatattg cggaatgaat aatgacaaat
     5401 tatgtgaatt gtaaaaatag tgaagggagc gggaactata gaaagttttt aaagagtgag
     5461 tggaagaata tcttattaat ttttatgggt ttcactgaaa ataataaagg cacccgaaag
     5521 tgcctttatg tttctgattt atcctttaaa gcaacggcgg atcaccacaa agaacaccgg
     5581 taggaagaag attgccagca acgttgcaga gaccattccg cccattaccc cgatacccac
     5641 tgcgttctgc gcgccactgc ctgcaccgtt actgatagct agcggtaata cgccgagaat
     5701 aaaggcgaga gaggtcatca ggataggacg cagacgcata cgtactgcca ttagtgtcgc
     5761 ttcaacaaca cctttaccct ctttctccat gagatcttta gcgaactcaa caatcaaaat
     5821 agcgtttttg gccgacaagc caattgtcgt tagcaagccc accataaagt agacgtcatt
     5881 tttttgatta aagagtgtcg ccgccagcag cacgccgaca atccctaacg gcacgaccaa
     5941 cataaccgag acaggaattg accagctttc atagagtgca gcaaggcaca ggaaaacaac
     6001 cacaaaggaa attgctacta gagcgggagc ctggtttccc gataagcgtt cctgatacga
     6061 catacccgtc cagtcataac caatgcccgc aggtaatttt gacgcaaggt tttccatcaa
     6121 cgccatggca tcgccggaac tggttcctgg cgcggcttcc ccctgaatct ccattgacgg
     6181 cagaccgttg tagcgttcca gtcgcggaga gccatacacc caatgtgaag tggtaaaggc
     6241 cgagaatggc accatttcgc cgttggcgct gcggacataa agtttatcga catcttctgg
     6301 cagcatacgg aatttggcat ccgcctgaac atacaccttt ttcacgcgac cacggtcgat
     6361 gaagtcgtta acgtaagtcc cacccaacgc cgttgaaatg gtctgattga tgtcagaaag
     6421 tgaaacactt aatgcctgcg ccttttcctg gtcaacttcc agtttaaact gcgcggtgtc
     6481 ttccaggcca ttaggacgca cgctgactaa gctggcagga tgttgcgccg ccataccaag
     6541 caactggtta cgggcctggg ttagggcatc gtgacccagc ccagcctgat caattaactc
     6601 aaagtcgaaa cccgttgccg tgcccagttc aacaatggct ggcatattga atggaatgac
     6661 aaaaccgtcg cggatcttgc ccaattccat tttggcacga tggattaccg cttccgcact
     6721 gttttcatca ccattacgct cttcccacgg tttcagactg acgaaggcca taccggcgtt
     6781 ttgtgcctgg ccgctgaagc taaagccgtt aaccgtaaag acactttcaa cgttcgcttt
     6841 ctcgttcttc agatagtaat ccgtcacttg atccaacact ttttgcgtcc gctcttgcgt
     6901 cgcgccagcg ggtaactgaa tcatggtcag aaagacaccc tgatcctctt caggtaagaa
     6961 ggaagacgga agacgtaaaa acaacaccac cattcctgca acaatcagcg catagatcag
     7021 taaatatcgt cctgtggaac cgaggatttt gccgacgctg ttggtgtagt ggttaacgct
     7081 atgatcgaag gtggtattaa accagccgaa gaaaccgccc ttattttcgt gatgctcagc
     7141 agagacgggt ttaagcagcg ttgcacataa cgcaggggta agaatcaatg ccaccagaac
     7201 agaaagtgcc attgccgaaa cgatggtgat agagaactgg cgataaattg ccccagtaga
     7261 accgccgaag aatgccatcg gaataaatac cgctgacagc accatcgcga tacccaccag
     7321 tgcgccctga atttgcgaca tcgatttttc cgtcgcttct tttggcggga gcttatcctc
     7381 catcatcacg cgctcgacgt tctccaccac cactatcgca tcatcgacga gcagcccgat
     7441 ggcaagcacc atcccgaaca tcgttagtgt gttgatggag taaccaaaag cggcgaggat
     7501 ggcaaacgtc cctaacaaca ccacgggtac cgcaatggtg gggatcagcg ttgctcgcat
     7561 attctgcaag aacagataca tcaccaggaa caccagcata atggcttcga acagcgtttt
     7621 taccacttcg tgaatagaaa gctggacgaa tggcgtggtg tcataagggt agagaacctt
     7681 cattccctgc gggaagaatg gctgtaattc cgccagtttt gccttaatgg ctttcgcggt
     7741 atcgagagca ttcgcgccgg ttgccagctt aatccccagg cccgccgccg gttttccgtt
     7801 gatacgagcg ataacgttat agttttcacc gccaagttca acccgtgcga catctttcag
     7861 gcgtaccacc gagccgtcac tgtttacgcg cagggtcact ttgccgaatt cttccggatt
     7921 ttttaaccgc gtttgagcaa taatcgaggc attcaactgt tgccctggta acgctggcgt
     7981 tccgcccaac tgtccggcag cgatctgatc gttctgtacc ttcaactggt taatcacatc
     8041 aaccggtgtc agtttatatt tgtttagcag atcggcgtcc agccagatac gcatcgcata
     8101 ctgtgcgccg aaaagctgta cgtcaccgac gccattcaga cggctaagcg tatctttaac
     8161 gttagaggcc acatagtccg agatatcgtc ctgtgtggtg cctgggttat cagagacaaa
     8221 acccgccacc atcaaatagc tgctactgga cttttcaaca ctgatcccct gctgctgaac
     8281 ctcctgcggc agcaacggcg tggcgagctg gagtttgttc tgcacctgca cttgcgcgat
     8341 atcaggatcg gtcccggact ggaaggtaag ggtaattgtc acgctaccgg cggaatcgtt
     8401 ggtggaggac atatacatca ggttatcgat accattcata ttctgttcga taacctgcgt
     8461 caccgtatcc tgcacggtct gcgcatcagc acctggatag tttgccgaaa cagaaaccgc
     8521 aggcggtgca atggttggat actgagcgac gggcaattgt aggatcgcca gtgcgcccgc
     8581 catcatcaga ataatggcca gcacccatgc aaatatcggt cgtcgaataa aaaagtttgc
     8641 catgtcagat taccttactt cgatgcagta tctgcggggg tatcggtagt ggctttaacc
     8701 tgctctcccg gacgcgcttt ttgcaggccg ctgacaatga cttgatcgcc agatttcaga
     8761 ccttcgctaa tcaaccattt atcgccaatc gcctgactgg caacgacagg gcgcgcttcc
     8821 acctgacttt tatcgttaac aatcagcacg gttgcatcac cacgcggtgt gcggctaacg
     8881 ccttgttgcg ggataagaat ggcgtcaggt tggacgcctt catcaatccg tgcacgcaca
     8941 tacatacccg gcaaaagcgt atgttgcggg ttagggaaga cagcacgtag ggttatggag
     9001 ccggtgcttt catcaacggt cacatcagag aattgcagcg tacctttcag gggataggtt
     9061 tgaccgtttt ccatgaccaa ctctacgttg ctggtggcgt tttccttatg caaatttcct
     9121 tgctctacgg attgcttcag cctcataaaa tcgttgctgg attgggtcac atcaacgtag
     9181 ataggatcga gctgctggac agtcgccagt tcagtcgttt gcccattagt gacaagagcg
     9241 ccttcggtca cggtcgattt gccgatacgt ccgctaattg gcgcagtcac tttggtataa
     9301 gcgagattga tgcgagcgct ttcgactgtg ccttttgcgg caatcacggc ggcatcggcc
     9361 tggcgagcat cagcaatggc ctggtcgtac tcctgctggc tgatgtattt cgtacccacg
     9421 agcggaacgt aacgttttac cgtcaaatgc gcgatggcgg cggcggcttc acttttcgcc
     9481 agttcgcctt tcgcgctgtt ataatttgcc tgataggtcg cgggatcgat ctggtacagg
     9541 gactggcctg cttgtacatc gctgccttca gtgaaattac gattcagtac gatcccgcta
     9601 acctgtgggc gaacttcggc tatacggtaa gcattggtgc ggcctggtaa ttcagtctta
     9661 acttctaacg gggccgtttt cacaatatga acggtaacct gcggttcacc gacgtgagct
     9721 ttctcttctc ccttatcgtt acaaccggcg attaaagccg cggagatcag aataaaggag
     9781 ggcaggagga aaaacctggc atgtttcgtc attactattc ctcaaaaaac caaaagcgcg
     9841 ttatttaccc agaaaggcaa cgcgttaact cgcagaaaga aaaatacagt ttgctatcct
     9901 acaaattatc attcgtcgat gtaaggaata gttatgaata ctggcgtctc aaggcacata
     9961 aacacaaaaa aagattaata ttctactgtt ttattttgac gcgggttgaa agaggcagaa
    10021 ttaaaacctc gtaaattgaa atatatattc atgtggcgaa tg
//
LOCUS       DAZPEB010000151        10001 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZPEB010000151 DAZPEB010000000
VERSION     DAZPEB010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10001
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.154"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..149
                     /locus_tag="ACHURJ_003186"
     CDS             <1..149
                     /locus_tag="ACHURJ_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282024.1"
                     /translation="ICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIRNVPWGASS
                     VKYS"
     gene            complement(266..1609)
                     /gene="gdhA"
                     /locus_tag="ACHURJ_003187"
     CDS             complement(266..1609)
                     /gene="gdhA"
                     /locus_tag="ACHURJ_003187"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFR1282025.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSESEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVGAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            1845..2117
                     /locus_tag="ACHURJ_003188"
     CDS             1845..2117
                     /locus_tag="ACHURJ_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFR1282026.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(2083..2490)
                     /gene="nudG"
                     /locus_tag="ACHURJ_003189"
     CDS             complement(2083..2490)
                     /gene="nudG"
                     /locus_tag="ACHURJ_003189"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFR1282027.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPTDIPLLEAFMALRAARAAD"
     gene            2577..3188
                     /locus_tag="ACHURJ_003190"
     CDS             2577..3188
                     /locus_tag="ACHURJ_003190"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFR1282028.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRELTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFISTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(3197..4504)
                     /gene="ynjE"
                     /locus_tag="ACHURJ_003191"
     CDS             complement(3197..4504)
                     /gene="ynjE"
                     /locus_tag="ACHURJ_003191"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFR1282029.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYAHAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(4571..5224)
                     /locus_tag="ACHURJ_003192"
     CDS             complement(4571..5224)
                     /locus_tag="ACHURJ_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR1282030.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(5224..6759)
                     /locus_tag="ACHURJ_003193"
                     /pseudo
     CDS             complement(5224..6759)
                     /locus_tag="ACHURJ_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter permease"
     gene            complement(6732..7898)
                     /locus_tag="ACHURJ_003194"
     CDS             complement(6732..7898)
                     /locus_tag="ACHURJ_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFR1282031.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPVV
                     WDDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLRRYGTH"
     gene            complement(7908..8456)
                     /locus_tag="ACHURJ_003195"
     CDS             complement(7908..8456)
                     /locus_tag="ACHURJ_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFR1282032.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            complement(8456..9163)
                     /locus_tag="ACHURJ_003196"
     CDS             complement(8456..9163)
                     /locus_tag="ACHURJ_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFR1282033.1"
                     /translation="MMKMQSRKIWYYCITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFISTGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            complement(9178..9855)
                     /gene="ydjY"
                     /locus_tag="ACHURJ_003197"
                     /pseudo
     CDS             complement(9178..9855)
                     /gene="ydjY"
                     /locus_tag="ACHURJ_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
     gene            complement(9860..>10001)
                     /locus_tag="ACHURJ_003198"
     CDS             complement(9860..>10001)
                     /locus_tag="ACHURJ_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFR1282034.1"
                     /translation="RFILQLCLAGLALFILVQLAKLYARHKHVDLSASRRSPLTHPKN
                     EG"
BASE COUNT         2386 a         2722 c         2583 g         2310 t
ORIGIN      
        1 atatctgctg ctatcgcaga aagttaacgg atgctgaact tgaagcgtgt tatcgttacg
       61 acaagcagtt ttgggagcac tacagtaaga aatgccgttg gcccataaga aatgttccct
      121 ggggagcatc atccgttaaa tactcataaa cgcctgaaat tttgccgggg gcgctgcgct
      181 tgcccggcct acattagagc gtaactgcaa tgaattgtgc ccatttgtag gcctgataag
      241 cgtagcgcca tcaggcattt acaacttaaa tcacaccctg cgccagcatc gcatcggcaa
      301 ccttcacaaa accggcaatg ttcgcgccct gcacgtagtt ggtttgctca ccttcaccac
      361 catgctcaac acaggcatgg tggatatcca gcatgatgtg atgcaaacgt gcgtcaactt
      421 tctcggcttt ccagcccagg cgcgcagcgt tttgcgccat ttcaaggcct gacgttgcta
      481 caccgccagc attagccgct ttacccggtg caaacagtac gcctgcctgc tggaacagtt
      541 cagtcgcttc gatggtggtc ggcatatttg ccccttcggc gacggcttta acgccattgg
      601 cgataagctg atgcgcggcg ccaacatcca gttcgttctg ggtggcgcaa ggcagggcga
      661 tatcaaccgg tacagaccac ggctgttggc cttcgagata gaccagacca aattctttgg
      721 cgtaatctgc cactcgacca tcgcggctgg ctttgatttc gataagacgt gccagtttct
      781 ctttcgtgaa tccgctttca tcaactacag tgccgctgga gtctgacgca gtgatcacac
      841 gagcaccaaa ttccatcgct ttttcgatag cgtactgggc gacgttgccg gagccagaaa
      901 cggaaacgcg catcccttca aaacccatac cgtggcgttt taacattgct tctgtgaaat
      961 aaaccagacc gtagccggta gcttccgggc gaataagact accgccaaat gaaaggccct
     1021 taccggtgaa gacgcaggcg gtattgttgg agagcttttt catcatcccc gccataaagc
     1081 cgacttcacg accaccaacg ccgatatcac ctgccggaac gtcagtatcc gcgcccagat
     1141 gacgataaag ctcggtcatc agcgcctggc agaaacgcat cacttcgctt tcgcttttac
     1201 ctttcgggtc aaaatcgcta ccgcctttac caccgcccat cggcagggta gtcagggcat
     1261 ttttgaaggt ttgttcgaag ccgaggaatt tgagaatgga gaggttaact gacggatgga
     1321 agcgcatacc gcctttgtac gggccgatgg ctgagctgaa ctgcacacgc catgcacggt
     1381 tgacctgtac ctggttgcga tcatcaaccc ataccacgcg aaactggatc acgcgctccg
     1441 gttcaaccag acgctccagt aatgacatct ggcgatattt tggattttgt tcaagaaaag
     1501 gccagagtgt ggtcattact tcacgaacgg cttgcgcgaa ctcggtttga ttcgggtcgc
     1561 gcttttggac atggttgagg aatgactcca gagaatatgt ctgatccata gatataaaac
     1621 ccttatatat taatacgatt gtgcttatgt tgtttatgtc atgtgctttt gcagttttcg
     1681 actataccat caagaatgtg acggtaagca agaaaaaaat gacccaggaa agcaaattaa
     1741 taacgagagt aatctcataa ccaaatgcga taactcagac gctaaagttt tgccacccgg
     1801 ctaccgttat cagttacaga gacaataacg ttaaggagtt ccctgtgagt cgagcattgt
     1861 tcgccgtagt attagcattt ccattaattg cactggctaa cccgcattat cgtccggacg
     1921 tggaggtaaa cgtaccgcca gaagtgttca gttcaggcgg gcagtcagcg cagccctgta
     1981 ctcagtgctg cgtttatcag gatcaaaatt attctgaagg ggcggtaatt aaagccgaag
     2041 gaatactgct gcaatgccag cgggatgaca aaacccttag cactaatccg ctgctctggc
     2101 ggcgcgtaaa gccataaacg cctctaatag tggaatgtca gtaggggcca gcggatattg
     2161 cagcgcctct tcaggtgagc accagaccag cgcctgatgt tcatgtgcct gtaacgtccc
     2221 gtggaagtcg ggtacgtgcc aggcatgaag atggataatc cggcccgaaa cttctcgctg
     2281 atggctggca acatactcac ccacagttgc ttcgatgccc aattcttcgt ttaactcacg
     2341 caccagtgcc tgctgctggc tttcatccag ctcgacttta ccaccggcaa actcccataa
     2401 tcccgcctga tcgctctggg cggggcgttg cgcgagtaaa attttgccat cacgttcaat
     2461 gatggcggca acaacttcaa tcattttcat aaattacaca cgggcagata agcgaaaaat
     2521 gccataatat ccgcactgtt gctcaatcag aaaggcttta tatttaagga gtgcctgtgc
     2581 tagaccgcca tcttcatccc cggattaaac cgttgttgca tcagtgcgtg cgggttcttg
     2641 ataaaccggg cattacgcca gatggtttaa cattagtggg atttgccatc ggcgtgctgg
     2701 ctctgccgtt tctggcgctg ggctggtatc tggcggcgct agtcgtcatt ttgttgaaca
     2761 ggctgcttga tggtctggac ggtgcgctgg cgcggcgcag agagcttacc gacgcgggcg
     2821 gctttcttga tatttctctc gattttctct tttacgcgct ggtgccgttt ggctttattc
     2881 ttgctgcacc ggagcaaaat gcgctggcag gcggctggtt gttgtttgcg tttatcagca
     2941 cgggcagcag ttttctcgcc tttgctgcac tggcagcaaa acatcagatt gataaccccg
     3001 gttatgcaca taagtcgttt tactatctgg ggggattaac cgaaggcaca gaaacgatct
     3061 tactgtttgt gctgggatgt ttattccctg catggtttgc gtggatcttc ggtgcgttgt
     3121 gctggatgac gacctttacg cgggtgtgga gtgggtatct gacgctgaag tcgctccagc
     3181 gtcagtaatt gcaatgttat ttgctactgt ccgggccgcg ttcaccggtt gctaccggat
     3241 tttttggatc gctgctccat tcgtaccagc cgccgtcata cacggaaaca ttcttccaac
     3301 ccatggcgtg tgcgtacata aaggtttcgg acgcgcgcca gccggtgccg cagtagaatg
     3361 aaacttgctg ctctggtttg atattccacg ctttccacat agcggtaata tcatcggcgc
     3421 tgcgcatggt gccatccggg ttatgaaaat cttccatatg cgtcgagtcg ctaccagcgt
     3481 gtccccaacg tgctccggct atttcacctt ttggtttaat atagctgtaa ccgctggtcg
     3541 taccgataaa ttctggccat gaacgaatgc tcaccagcga tgcatcctgg cgatgcagca
     3601 gtccacgggc ctgttccata tcgagcatca actgcggttg tgccgggatc ttcacgccga
     3661 aatccggttc cgctttcact ttcggtggcg ttcctcgctc aacaggcagt cccgcgtcgg
     3721 accaggtttg ccagccgcca tccagcagac gcacatcttt cacgcctgcg tagagcataa
     3781 tttgcgccac acgcgctcca gcgtatacgt cacgcccgta cagaatgacc gtggtgtcat
     3841 ggcgaatgcc gtgttttgcc agcatcgctt ttagttgttc atcagaaact ttgttccaca
     3901 gcggttcgct ttccacttcg ttggtatcga tgtagtcagc accgggaata tggctgataa
     3961 ggtaaagctt aggagcgccc caggccgctt caatgacttt ccagtcaccg gcaggtttcg
     4021 ccgtaacctc tttaccttgt tgcaggtcgt gcagccattg cggataaacc agctgctcaa
     4081 agtgcggcag tttttgtaga cgggaaggtt cgcttagcgc gtcactcaga atggagatat
     4141 gcgtaaaacc tgctttttgc agtcgcgttt tgacggcgtc gacatctttg tcattaccgt
     4201 acagcgccac cggggcatcg gctttcaggt tatgttgctt gatccacgcg ttgagctgtt
     4261 cggtgctcat tttgtcgagc cagctggcag agaggtttaa ggcggcaggt tcatgaccag
     4321 aagggccatt taaggtttgt ggccagccgt tataaaacgc gctggggcga gtatctatcg
     4381 ctttgccatt ttgttgttga agctggtcaa gtgtaagagg cttcgccagt tcagcggccc
     4441 acgaagaagc gcaagccagc cctaaagcca ttgccagcgc ggtcatttga gaaacacgtt
     4501 tcatcgaaaa tcccgacatt gtagttttga gtgcgccatt ctgaactgac gcattgagaa
     4561 aaactttgcg ttatcgcagt ttgttgtaat tttctgacca ctgcgccata tccagaacag
     4621 aactatcagc aggaacatcc tggagatcgt gcgttacctg aacgacgggg atcgccagtt
     4681 cgcgaacttc gctgaacacc cactggcgaa aattatcgcg cagagccaca tcaagacggc
     4741 tgaatggctc atccaggagt aacgcttttg gctgagcgag aagggcgcgc agcagggcca
     4801 cccgcgctcg ctgaccgcct gataatgtcg cagggtcctg gtgataagtt tcagccagtc
     4861 ccgctcgatc aagcgcgtct ttcacggcat tgcgcctggc agtccccttg agcgtcgacg
     4921 gtagcgccag cagtaaattt tgcccgacgc tgaactggtc aaataacagt gcatcctgga
     4981 aaagaatgcc aatctgacgc tgtgcggtgg gtagcatgtc aatccgttgc tcattgagcc
     5041 atagctcacc tgtacaagaa aactgtccag ccagtgcacc aatcatccat gaaaacagag
     5101 tggattttcc acagccagac ggccccatta acgtgacgat gtcaccttta tcaaccgtaa
     5161 agttaacgtt tgtcagcaag cggctttctg gtaaacgtag cgaaacattt ttcacgcaga
     5221 gcattagcgg agtccttgtc tgacataacc tacccatttt gcgactaacg cggtcagggc
     5281 aaaaataata agcggtaaca acagttgcca taaagcctgg gcggcgagaa taccgttgct
     5341 gccgccgctg cttaatgcca ccgcctcagt ggtgagcgtc ggaaaacgcc ccgcgcccag
     5401 ccacagcgtc ggcatatact gcgcaatact gactgaaaat cccaccgcga aggcaatcag
     5461 cacagggcgc aacatgagtg tgcatttcac gtaaaagaag attttggccc gctaccagcc
     5521 cagtgtttgt gcaatcaaaa ttaaccgtga atcaatgcgc tgccaggcgg gttgcaggat
     5581 aaacagcatc cacggcatta cccacagcag atgcccccag accaccgccg tccagcttcc
     5641 atccaggttc agccatagcg ccagcgtgta ctggcccgcc accagtggca gagcgggcaa
     5701 tagaatgggc aaccatagcc acaactggcg acgctgtggc ccccattcca gccacagcag
     5761 taacaggagc aaagcgatga atgttgctac cagccccatt gtcagactgt tgatgagtgc
     5821 ttcactattg atcgtcgact gatccgcgag gatcgccagc agaaccacac acagcacacc
     5881 ggttaagggt aaaaaactcg ccagcgtagt gcctggtaat aaaggtgtgg caggcttgcg
     5941 aatgccatct acgcggggaa tagtgcgccg ccagctgcgc catagcagat agctcagcaa
     6001 aacgtaggcg gcgagtaaca gcatcagcag cagactggcg agtgcgcctt tcgtttgttg
     6061 atccgcgtcg ccctgagtta accactgcca gctaattact gccagcgtcg gtggattacc
     6121 tggcccaaga ataatcgcca catccacgac cgaaagcgac caggcaacaa tcgccagcat
     6181 cgccattgcc agcgaaggcg cgacggaggg caacagcagc cagttcaggc attgccagcg
     6241 gctgtagccc agcgaatcca gcacaatgac ctgctgcaac agccgttttt cgctcaatac
     6301 tgccgctaag atccacagca gaaatgcgct ttctttcact gcaagggtga ggcccagacc
     6361 gatgccaaat cggtccattg gcggagtgaa atacgggaaa tagtcatata gcagccctcc
     6421 gtcagcaaag agcagcaggg cgctggtggc aaaagccaca tggggaatgg cgagcagcca
     6481 cggcagacgg gcgcacatac gctgccattt cggcccaggc catagcgcca caatcaccag
     6541 cagggcaatc aacaatgccc cgaccgccgc gatggttgtc gacaccagcg ttgccagtaa
     6601 tgcatgcggt aactgtggat cggcaaataa cgcctgccag tgtgtcaacg acaacgcagg
     6661 cgatatcagc gtgagagctg ccgggatcag cggtgcataa atcaccgcca ccatcgccca
     6721 cagcaaaaag attaatgcgt accgtaacgg cgtaaccatt cttgttccag tgcattcacc
     6781 catcctgcgt gcggttcagc cagtaccggc ggcagatcct gcggcattct tgattgcaat
     6841 atttcgcgct gcccgtcagg cagtttttgc ggatcgagaa cagaaggatc gtcccagaca
     6901 acgggatctg ctttacgcag ttgcgcatcg ggtgagagca gaaaattggc aactaccttc
     6961 gccgcagcac tggcattcgc gttggctgga atggtgacga aatgcacgtt gccaatcatc
     7021 ccctcgcgaa agccaaaact gtaactgctt gccggcaaat cgccgctggc gattttttgc
     7081 tgcgcatgcg cggggttaaa ggtcagcgac aggcgcaatg tgccggcttt cagcagagca
     7141 tccatccgcg cgggcgaagg cgggaaatcc tttccttcgc gccacaaata cggatgcagt
     7201 gcatcaagat attgccacaa gggcgcagtg acacgggcga aagtcgcatc gtccggcgct
     7261 tcttttaatg cggcgggatc tggcgtcagc ataatcagca actgttcaag aaacgccgtg
     7321 ccggtaaagt ccggtgggcg cggataagta accgtgccgg gattggcttt agcaaactcc
     7381 agtaaggctt gcggcgtttg tggtggctgt ggcgtaacat cgcggcgggc gataaacgtc
     7441 agttgtgcgc cgccccaggg cgattccgca ccttgtgtcg gcactgaaaa atcttcccgc
     7501 accggcagct gtgtgtcgac atagcgccag ttgggcagag tctccgccca gcccgtttgt
     7561 aataaattgg cctcttttaa ggtgcggaag ttttcgccgt tcacccagag cagatccacc
     7621 gagccgcccg ttttacgacc ggcagcagct tcggtctgaa tgcgcttcac cgcgtctgcg
     7681 gcatccgcga gacgaacaat cttcaggttt atagcgtaat gggttttcat ctcgccgcta
     7741 acccagtcga gatagcggtt aattgcggta tcgccgcccc aggcgttaaa ccagacggtt
     7801 tgccctttgg cctcattttt aatttcctgc cagtcactgg catgtgttgc caaagaaaac
     7861 agcgataaca atcccagcaa ccacccacaa tggcgcatct tctctcctta agaacgtttt
     7921 tccggaatac ggcacagccc ctgagcggga atgtccatgg cagaattaaa acgagccgac
     7981 aggttttgca ggccgatcaa tgccgtcagt tcggtgagcg cctgagcgtc aaaatgcgca
     8041 gccagtcggg tacgcagggc atcatcgact gttggcggtg tgacgcttgc ggcttcggcg
     8101 tactccagcg ccagccgttc ttcatcgcta aagagcgggc tttgccgcca gtcagccact
     8161 gccagcagtt tatcggtgct gccggtacgc tcggcgactt tcatactggt gatatccaca
     8221 caaaactcac acaggcacat ttgcgcaatg cgcgcgctga caagcgatcg cactaccgga
     8281 tcgagcggtg agcgtttgcg ctccagccac ccaacaaaca ttgacaccaa ataaaagata
     8341 aacgggatcc gcccccacca gcgaatcggg cttagcacct cgccgtaatg gcgacgatgc
     8401 agccacgcct gcggacgtaa aatgaaagga attttgctaa gcggcggtaa acccatcagt
     8461 cattcctctt ctggcgctca agccatattt tcttcgccat aaaaatcacc acggtcaggg
     8521 caaacagaat aaacagtccg gtgacaaacc agaaagtacc gcctgttaac atgctgcccg
     8581 cccaggaata aacaatagtc gccggtaatt gaccaagccc ggtggagata aaaaacgagc
     8641 gaaaacgtat tgaagtcaga ccggcagcat agctgattgg atcgaaaggg acaaaaggca
     8701 ataaccgaca gaccaggatg gtgtgtttgc cgtagcgagt gaaaaagccg tccatactgt
     8761 caagcacggt tttgccggtt aatttttcca ccacttcgcg gcccatcact ctggcgataa
     8821 aaaagcacag cgccgcgccg gccatcgaac tggtccacga cagcaagccg ccccagaacg
     8881 cgccaaacag cgacgcattg gcaaaggtga tcaaaaacgc aggtagcggt gcagcaatgg
     8941 cctgtaaaat catcaacaag aacgagacaa ctgccgccag tgcgccgtaa gactgaataa
     9001 agcgttctat accctgttgg tccacggcgg caaacgccgc aacgctgcga ttgataaact
     9061 catggacgcc gggaagcagc gcccatgcga gcagcatagc gaacaacagg atgatgaggg
     9121 ttatacagta gtaccagatt ttacgcgatt gcatcttcat catcctttat cctggcttta
     9181 ttcggtgatt ttaaaggtaa ccgttgccag cgtgttatcc gccgggagaa ctgaggcatt
     9241 gcctttgaat ttcacttcgc cgcgtttttc caccgcacca taagtgtatg ttgcattgct
     9301 gacgatgccg accgggcagc tatccagaca caccagtcaa cccgttttct tctcttctgc
     9361 tgccgttaaa ttaccgccaa agcgcatgtc cagtttcttg ccattactgt caacaatcac
     9421 ttcatcgaag gaatacgctt ttgccgcccc ttgccagttg accgaaatat ccagtttgct
     9481 gcctgtgaca tgagtcgttt ctttattatc catcgtcatg ttttcgcccg gcgtaccacc
     9541 tgcctctttc agggcttcat aaaatgcttt cggggtcgca taagccataa acagcgattt
     9601 atgtccgttg gagccatctt taaacacaat accgtgacga gtgtcgtcgg tgaggaaacg
     9661 accattaatt tgcaccagca tggttaccgt atggttattg gcatcgacgc gcagcggttg
     9721 gctgttcgaa agtccgtcgt attccacttt tgccgccgaa ttctcttttt gatcgcagcc
     9781 gctaagcccg gcaacagcca gtaagcagag tgcagccagt ccttttttcc atgacactga
     9841 ataatgttgc gacatcgttc tatccttcat tttttgggtg agtaagcggg ctgcggcgcg
     9901 aagcagacag atccacatgt ttgtgtcggg cgtagagttt tgcgagctgg acgagaataa
     9961 acagcgccag gcccgccaga cagagttgta aaataaagcg c
//
LOCUS       DAZPEB010000152         9955 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZPEB010000152 DAZPEB010000000
VERSION     DAZPEB010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9955
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.155"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..456
                     /locus_tag="ACHURJ_003199"
     CDS             <1..456
                     /locus_tag="ACHURJ_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shiE"
                     /protein_id="HFR1282035.1"
                     /translation="HKKHVSEKETWRWVEGYNKATELARQCPDTHVLSISDREGDFYD
                     LFERAEQTPGIKADWLVRMKFNNRATLNINGKRDHRLLHERIMEITPQQLVEFTIPDG
                     RGQQARTLPDMLSSDAGPLSYPLIYSSKVMIISLFDSTVMPDKYRHNGK"
     gene            complement(406..1599)
                     /locus_tag="ACHURJ_003200"
     CDS             complement(406..1599)
                     /locus_tag="ACHURJ_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1282036.1"
                     /translation="MSPGIEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFVTDTTAISQQVAWLLGLSAVAALIAQFGV
                     VRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            1735..3459
                     /gene="iucA"
                     /locus_tag="ACHURJ_003201"
     CDS             1735..3459
                     /gene="iucA"
                     /locus_tag="ACHURJ_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HFR1282037.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3460..4407
                     /gene="iucB"
                     /locus_tag="ACHURJ_003202"
     CDS             3460..4407
                     /gene="iucB"
                     /locus_tag="ACHURJ_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase IucB"
                     /protein_id="HFR1282038.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4407..6149
                     /gene="iucC"
                     /locus_tag="ACHURJ_003203"
     CDS             4407..6149
                     /gene="iucC"
                     /locus_tag="ACHURJ_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIS family aerobactin synthetase IucC"
                     /protein_id="HFR1282039.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFVEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDATSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6146..7483
                     /gene="iucD"
                     /locus_tag="ACHURJ_003204"
     CDS             6146..7483
                     /gene="iucD"
                     /locus_tag="ACHURJ_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase
                     IucD"
                     /protein_id="HFR1282040.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFARNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVITLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7489..9684
                     /gene="iutA"
                     /locus_tag="ACHURJ_003205"
     CDS             7489..9684
                     /gene="iutA"
                     /locus_tag="ACHURJ_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HFR1282041.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLSLTKSVNVSDSKLEGVKVDSYELGWRFTGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRIQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(9670..>9955)
                     /locus_tag="ACHURJ_003206"
     CDS             complement(9670..>9955)
                     /locus_tag="ACHURJ_003206"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282042.1"
                     /translation="RSSSQEFQDLPDGAETTLTCKVSFQTDNEMRICDKFQSKIHKTE
                     IMLFSGEQSIPETEYLVKAVYYTGCKQHGHQGPCCLQHTLVAKASPSEQH"
BASE COUNT         2396 a         2564 c         2644 g         2351 t
ORIGIN      
        1 cataaaaaac atgtcagtga aaaagaaacc tggcgttggg ttgaaggata taataaagca
       61 acagaactgg ctcgccaatg ccctgatacc catgtattgt caatttctga tcgtgaaggc
      121 gatttttatg acctgtttga acgcgcagag cagactcccg gaataaaagc tgactggctt
      181 gtcagaatga aatttaataa cagagcaacg ctgaatatta atggtaaacg ggatcatagg
      241 ttgttgcacg aaagaatcat ggagattaca ccgcagcaac tggttgaatt cactatacct
      301 gatgggcggg ggcagcaagc cagaacactg cctgacatgc tttcatcaga cgcaggtcct
      361 ctctcatatc ctctgattta ctccagtaag gtgatgatca tatctttatt tgatagtacg
      421 gtaatgccag ataaatacag acacaatggt aaataatata gttgcaaata atgctgccat
      481 aatcagcgcg attgcgacac ctgttttcga taccagcggc accagcaaag cgcacagtcc
      541 atagccaagt gtgtgactgg tagcgagcca gcccgcgcct gcgccatcag ccagcttatc
      601 attcagtaga agttgataag cgggtgttgc cagagctgct ccaaatgaca gcactgcaca
      661 gcccgggtaa aacaaccata gctgttcgga gagcatgata gccagaccac cactcatcaa
      721 cactccggca ctcaggagca gggccaccgg agtcaggcgc tgcggacgga ctaccccgaa
      781 ctgcgcgata agcgcagcta ctgcggacag acccaacagc caggccacct gttggctaat
      841 ggcggtggta tccgtgacga actggcgagt aagggcaggc gaaagtccaa gctgcatcat
      901 gctcaccgcc gcagccagta atattgcgca aagcagataa ggcagacaat cccgcttcag
      961 actgacgctc ttgcactccg gtgtgggctg tggtggtgtg ccgggcaacc gcagcagcat
     1021 cagcagcgcc agcactggtg ctgccatcag tagccccagt ggcgccagag gatgaatggc
     1081 cagcattgct gccgcgcaca gcggcccgaa tagcctgccg caactcaggc cggagctgat
     1141 ggttgccaga gcggccatgc gattcccttc tccagctctc tgcaatgccc agacctgaca
     1201 ggctggcacc attgccgaca cggtcagccc gtaggcgatc cgggcgatga ttaatattcc
     1261 cagtcccacc gctgttgtta tggcgctggt agccatcagc acgcttccca gccctagcag
     1321 ggtaaagctt atgccatatc ctcccagagc ccacaacacc acaggtttac tgccagtacg
     1381 ggaaatttgc tttccccacc acggggacga tggcagaaac agcattgagc caagcatcag
     1441 cagggcagcc catacagaca gactcagatt tgtctgtatg accagaacag ggagcacaac
     1501 cagcagaccg ttctgtccga taccgagaag cccggcactg aacgcaagtg gccagcagga
     1561 cagtgttttt tggggcgtat cttcgatccc tggtgacatt ataatgcttc aactccatgt
     1621 attaattgtg tttatttgta aaattaattt atctgacaat aacatttatt gttgataatg
     1681 agaatcatta tcgacataat tgttattatt ttactgtgtg ggagctgttt gactatgacc
     1741 ctgccctctg aaaaaccagc cacagatgtg gctgcgcagt gcttcctgaa tgcactgatt
     1801 cgtgaaacca cagactggaa actgacagaa tacccgccag acgaattgct catcccgctg
     1861 gatgagcaga aatcgctcca tttcagagtg gcttatttct ccccaaccca gcatcaccgc
     1921 tttgcatttc ctgcacgtct ggtcacggca tcaggcagtt atcctgtcga ctttaccact
     1981 ctctcccggc ttattattga taagctacgc catcaactgt ttctgcccgt tcccctctgc
     2041 gaaactttcc accagcgcgt gctggaaagc catgtccata cgcaacaggc aattgatgcc
     2101 cgtcatgact gggccgctct gcgtgaaaaa gcgttgaatt ttggcgaggc tgagcaggcg
     2161 ctgctgacag gacacgcttt ccaccctgcg cctaagtctc atgaaccgtt taaccggcgg
     2221 gaggctgaac gctacctgcc tgatatggca ccccacttcc cactgcggtg gttttcggtg
     2281 gataaaacgc aaatcgccgg tgaaagtttg catctcaacc ttcaacagcg gctgacgcga
     2341 tttgccgcag agaatgcgcc tcagttactc aacgaattaa gtgacaacca atggctgttc
     2401 ccgttgcacc cgtggcaggg ggaatatctt ttgcagcagg ggtggtgcca ggcacttgtt
     2461 gctaaagggc tgattaaaga cttaggtgag gccggcacgt cgtggctgcc gaccacctct
     2521 tcccgttccc tctactgtgc caccagccgc gatatgatca agttctccct gagcgtacgg
     2581 ctgaccaact ccatccgtac tctgtccgtg aaagaagtga agcgtgggat gcgcctggca
     2641 cgcctggctc aaaccgacgg ctggcagatg ctacaggtcc gcttcccgac tttccgggta
     2701 atgcaggagg atggctgggc cgggctgctc gatcttaacg gcaacatcat gcaggaaagt
     2761 ctgtttgccc tgcgtgaaaa tctgctggtg gatcagccga aaagccagac taacgtactg
     2821 gtctccctga ctcaggccgc acctgatggc ggtgattcgc tgctggtttc ggcggtaaaa
     2881 cgcctgagcg atcgcctcgg tatcactgtg caacaggccg cccatgcatg ggtcgatgca
     2941 tactgtcagc aagtgctaaa gccgctgttt acggctgaag cggattacgg cctggtgctg
     3001 ctggcgcatc agcaaaatat tcttgtccag atgcttgggg atctgccggt cggatttatt
     3061 taccgtgact gtcagggcag cgcttttatg cctcacgcga cagactggct cgattctatt
     3121 ggcgaggcgc aggcggaaaa tattttcacc catgagcagt tgctgcgcta tttcccttat
     3181 tacctgctgg ttaactccac ttttgctgtg accgctgcgc tgggggctgc cgggctggac
     3241 agcgaatcga atctgatggc tcgtgtacga gcatcgctgg ctgaagtgcg tgatcaggtg
     3301 actcataaaa cctgtctcaa ctacgtgctg gaaagtccgt actggaacgt aaaaggtaac
     3361 tttttctgtt atctgaacga tcataacgag aacaccattg ttgacccttc ggtgatctat
     3421 ttcgatttcg ctaacccgct gcaggctcag gaggtctgaa tgtctgaggc aaacattatt
     3481 cacagcagat atggactgcg ctgtgaaaaa ctcgacaagc ccctgaatct tggctgggga
     3541 ctggacaata gcgcggtgtt gcactgtccc ggggagctgc cgacagggtg gctgtgcgat
     3601 gcgctggatc agatatttat cgccgcacca caactgtcag cagtggctct cccctgggcc
     3661 gaatggcgtg aggagccaca ggcgctgacg ctttttgggc aggtaaaaag cgacattatc
     3721 catcgtactg ccttctggca gttaccgtta tggttgagtt caccggcaaa ccgggcctcc
     3781 ggtgaaatgg tttttgatgc agagcgtgag atttatttcc cgcagcgccc accccgtcca
     3841 cagggtgagg tttaccgccg ttacgatcca cgtattcgca ggatgctgag tttccgcatt
     3901 gccgatcccg tttctgatgc agaacgtttc acccgctgga tgaatgatcc gcgcgttgag
     3961 tatttctggg agcaaagtgg ctcactggag gtacagaccg cctatctgga gcgccagctc
     4021 accggtaaac atgcattccc gctgattggg tgcttcgacg atcggccatt cagctatttc
     4081 gaaatctact gggcggcgga agaccgcatt ggtcgtcact attcgtggca gccctttgac
     4141 cgtggcctgc atctgctggt tggtgaacag caatggcgcg gggcccacta tgtgcaaagc
     4201 tggctgcgcg ggctgacaca ttacctgctg ctggatgagc cccgtacgca gcgcacagta
     4261 ctggagccac gcaccgataa ccagcgcctg ttccgccatc ttgagcctgc gggataccgg
     4321 acaattaaag agttcgactt cccacataag cgctcgcgca tggtgatggc ggatcgtcat
     4381 cacttcttca cggaggtcgg tctgtaatga atcacaagga ctgggatttt gttaaccgtc
     4441 ggctggtggc aaaaatgttg tctgagatgg agtatgagca ggttttccac gcggaatctc
     4501 agggcgatga ccactactgc attaacctgc cgggagcaca atggcgcttc atcgctgaac
     4561 gtggtatctg gggctggctg tggattgatg cccaaacgtt gcgctgcacg gacgaaccag
     4621 tactggcgca gacgctgctg atgcagctaa agccggtgct gtcaatgagt gatgccactg
     4681 ttgcagagca tatgcaggat ttgtatgcca cgctactggg cgacctgcaa ctgctgaagg
     4741 cccgtcgcgg actgagtgcc agtgacctga ttaatcttga tgccgatcgt ctgcaatgcc
     4801 tgctgagcgg tcatcctaaa ttcgttttta ataaaggtcg ccgtggctgg ggcaaagagg
     4861 cgctggaacg atatgcgcca gagtatacca acaccttcag actgcactgg ctggcggtaa
     4921 aacgtgaaca tatgatctgg cgctgcgata acgatctgga tattcagcag ttgttgactg
     4981 ccgcaatgga tccgcaggag tttacacgct tcagtcaggt ctggcaggaa aacgggctgg
     5041 atcacaactg gctaccgctg ccggtacatc cgtggcagtg gcagcaaaaa atcgccacag
     5101 acttcatcgc tgattttgtc gaaggcagga tggtgtctct cggcgagttt ggcgaccagt
     5161 ggctcgccca gcagtcgctg cgtaccctga ccaacgccag ccgccgggga gggctggata
     5221 tcaagctgcc gctgaccatc tacaacacct catgctaccg ggggatccct ggcagatata
     5281 tcgctgccgg accactggct tcacgctggc tgcaacaggt ttttgcgacc gacgccaccc
     5341 tggtgcaaag cggcgcagtg atccttggtg aaccggcggc aggctatgtg tcccatgaag
     5401 gctatgccgc gcttgcccgg gctccctatc gctaccagga aatgcttggt gttatctggc
     5461 gggagaatcc gtgccgctgg ctgaaaccgg atgaaagtcc tgttctgatg gcaacactga
     5521 tggagtgcga cgaaaacaat cagccgctgg caggcgcata tatcgaccgc tccggactgg
     5581 acgctgaaac ctggcttacg caattgttcc gggtggttgt ggttccgctg tatcacctgc
     5641 tttgccgcta cggtgtcgcg cttattgcac acggacaaaa tataacgctc gccatgaaag
     5701 agggggtacc acagcgtgtt ctgctgaaag acttccaggg cgatatgcgg ctggtgaaag
     5761 aagcgtttcc cgaaatggat tctttgcctc aggaggtgcg tgatgccaca tcccgtctga
     5821 gtgcggacta cttaatccat gatttgcaga cgggtcactt cgtgacagta ctgcgtttta
     5881 tttcgccact gatggttcgt cttggcgtac ctgaaaggcg attttatcaa ctgctggcag
     5941 cagtgttgag tgattacatg aacaaacatc cacaaatggc agagcgtttt gcgcttttct
     6001 cactcttcag gccacaaatc attcgcgtgg tgctgaaccc ggtaaaactg acctggccgg
     6061 acctggatgg cggcagtcgc atgctgccga attaccttga gaatctgcaa aatccgctgt
     6121 ggctggtaac tcaggaatat gaatcatgaa aaaaagtgtc gattttattg gtgtcgggac
     6181 agggccattt aatctcagca ttgctgcatt gtcacatcag atcgaagaac tgaactgtct
     6241 cttctttgac gaacatcctc atttttcctg gcatccgggt atgctggtac cggattgtca
     6301 tatgcagacc gtctttctga aagatctggt cagtgcagtt gcacctacaa atccctacag
     6361 ctttgttaac tatctggtga agcacaaaaa gttctatcgc ttccttacaa gcaggctacg
     6421 tacagtatcc cgtgaagagt tttctgacta tctccgctgg gctgctgaag atatgaataa
     6481 cctgtatttc agtcataccg ttgaaaatat tgatttcgac aaaaaaagtc gattgtttct
     6541 ggttcagacc agtcggggag aatattttgc ccgcaatatc tgccttggta caggaaaaca
     6601 accttattta ccaccctgtg tgaagcatgt gacacaatcc tgtttccatg ccagtgaaat
     6661 gaatcttcgt cggccggacc tgagtggaaa acggataacc gtggttggtg gaggacagag
     6721 tggtgcagac ctgttcctta atgcattacg cggggaatgg ggagaagcgg cggaaataaa
     6781 ctgggtctca cggcgtaata attttaacgc actggatgag gctgcttttg ctgatgagta
     6841 ttttacacct gaatatattt caggcttctc cggactgaag gaagatattc gccatcagtt
     6901 actggatgag cagaaaatga catcggatgg catcactgcc gattctttac tgaccattta
     6961 tcgtgagttg taccaccgtt ttgaagttct gagaaaacca agaaatatcc gtctgctacc
     7021 cagtcgctcg gtaataactc tggaaagtag tggtccgggc tggaggttat tgatggaaca
     7081 tcatctggat cagggcagag agagcctgga aagtgatgtg gtgattttcg ccacaggtta
     7141 ccgttctgca ttgccacaaa tacttccctc actgatgccc ttgatcacca tgcacgataa
     7201 gaacaccttt aaagtgcgtg atgacttcac tctggaatgg agtggcccga aagaaaataa
     7261 catctttgcg gtcaacgcca gcatgcaaac tcatggcatc gccgaacccc agctcagcct
     7321 gatggcctgg cgatctgcac gtattcttaa tcgcgtactg gggcgtgatt tattcgatct
     7381 cagtatgccg cccgcactga ttcagtggcg cagcggcagc cggaaaaaac cgcagccgga
     7441 ggctgcttct ttaactcact atacagcaaa tattcaggaa taacgatgat gcgcaaaaag
     7501 tatatgcccc gggctcttgg tccgctgctt cttgtcgtgc tgtcaccagc tgtcgcccag
     7561 caaaacgatg ataatgagat catagtgtct gccagccgca gcaatcgaac tgtagcggag
     7621 atggcgcaaa ccacttgggt tatcgaaaat gccgaactgg agcagcagat tcagggcggt
     7681 aaagagctga aagacgcact ggctcagtta atccccggcc ttgatgtcag cagccagagt
     7741 cgaaccaact acggtatgaa catgcgtggc cgcccgctgg ttgtcctgat tgacggtgtg
     7801 cgcctcaact cttcacgttc cgacagccga caactggact ctgtcgatcc ttttaatatc
     7861 gaccatattg aagtgatctc cggcgcgacg gccctgtacg gtggcgggag taccggaggg
     7921 ttgatcaaca tcgtgaccaa aaaaggtcag ccggaaacca tgatggagtt tgaggctggc
     7981 acaaaaagtg gctttaacag cagtaaagat cacgatgagc gcattgccgg tgctgtctcc
     8041 ggcggaaatg accatatctc cggacgtctt tccgtggcat atcagaaatt tggcggctgg
     8101 tttgacggta acggcgatgc caccctgctt gataacaccc agaccggcct gcagcactcc
     8161 aatcggctgg acatcatggg aaccggtacg ctgaacatcg atgaatcccg gcagcttcaa
     8221 ctgataacgc agtactataa aagtcaggga gacgacaatt acgggcttaa tctcgggaaa
     8281 ggcttttccg ccatcagcgg gagcagcaca ccatacgtca gtaaggggct gaattctgac
     8341 cgcattcccg gcactgagcg gcatttgatc agcctgcagt actctgacag tgatttcctg
     8401 ggacaggaac tggtcggtca ggtttactac cgcgatgagt cgttgcggta ctacccgttc
     8461 ccgacggtaa atgcgaataa acaggcgacg gctttctcct cgtcacagca ggataccgac
     8521 cagtacggca tgaaactgac tctgaacagc caactgatgg acggctggca aatcacctgg
     8581 gggctggatg ctgagcatga gcgctttacc tccaaccaga tgttcttcga tctggctcag
     8641 gcaagtgctt ccggagggct gaacaaccat aagatttaca ccaccgggcg ctatccgtca
     8701 tatgacatca ccaatctggc ggccttcctg caatccagct atgacattaa tgatattttt
     8761 accgttagcg gtggcgtacg ctatcagtat actgagaaca gggtagatga tttcatcgac
     8821 tacacgcagc aacagaagat tgctgccggg aaggcgatat ctgccgacgc cattcctggt
     8881 ggttcggtag attacgataa ctttctgttc aatgctggtc tgctgatgca catcaccgaa
     8941 cgtcagcagg catggttcaa tttttcccag ggggtggcat tgccggatcc ggggaaatat
     9001 tatggtcgcg gcatctatgg tgcagcagtg aacggccatc tttccctgac aaagagcgtg
     9061 aacgtcagcg acagtaagct ggaaggcgtg aaagtcgatt cttatgaact gggctggcgc
     9121 tttaccggtg acaacctgcg gactcaaatc gcggcatatt actcgctttc caataagagc
     9181 gtggaaagga ataaagatct gaccatcagt gtgaaggacg acaggcgccg tatttacggc
     9241 gtggaaggtg cggtggacta cctgatcccg gatactgact ggagtaccgg tgtgaacttc
     9301 aatgtgctga aaaccgagtc gaaagtgaac ggtcaatggc aaaaatatga cgtgaaggaa
     9361 tcaagtccat cgaaagcgac agcttacatt aactgggcgc cggaaccgtg gagtctgcgt
     9421 atacagagca ccacttcttt cgacgtaagc gatgcagagg gtaacgatat taatggttac
     9481 actaccgtcg attttatcag tagttggcag cttccggtgg gaacactcag cttcagcgtt
     9541 gagaacctct tcgaccgtga ctataccact gtctggggac agcgtgcacc tctgtactac
     9601 agcccgggtt acggccctgc ttcactgtac gactacaaag gccggggccg aacctttggt
     9661 ctgaactact cagtgctgtt ctgacgggga tgcttttgca acaagggtat gctgtaaaca
     9721 acatggtcct tggtggccat gttgtttaca gccagtataa tatacagcct taacaagata
     9781 ttccgtctcc ggaatggact gttcaccact gaataacatt atttctgttt tatggatctt
     9841 tgactgaaat ttatcgcata tcctcatttc attgtccgtc tgaaaactaa ccttacaagt
     9901 aagcgtcgtc tcagcaccgt ctggcagatc ctgaaattcc tgagaggatg atctg
//
LOCUS       DAZPEB010000153         9780 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZPEB010000153 DAZPEB010000000
VERSION     DAZPEB010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9780
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.156"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(9..1907)
                     /gene="espL1"
                     /locus_tag="ACHURJ_003207"
     CDS             complement(9..1907)
                     /gene="espL1"
                     /locus_tag="ACHURJ_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HFR1282043.1"
                     /translation="MSQNDIIIRTHYKSPHRLHIDSDIPTPSSELINQFARQLITLLD
                     TSDLSSMLSYCITQEFTANCRKISQNCYSTALFIINFATSPIHAENILITLHYKKEII
                     PLFLETTPIKANHLRSILDYIEQEQLTAEDRNHCMKLSKKIHREKTIQPTVNLNGSAC
                     FLQSPSDAIFCRHLSLQYALDSLRNGKGKVNLIKHYSSVESIQQHVPLVRDAEFRALL
                     RHPPAGSRVIASKDFGFALDIFFCRMMANNVSHMSAILYIDNHTLSVRLRIKQLVYGQ
                     LNYVVSVYDPNDTNVAVRGTHRTARGFLSLDKFISSGPDAQTWADRYVRNCAIAILPL
                     LPVGVPGAIFAGIASRMPFAPIHPSAMLLIMATGQTQQLITLFKQLPILPEKEIIEII
                     TAQNSVGTPALFLAMMNGHTDNVKIFMQEIQSLIYNHIIHEDNLVKLLQTKSANETPG
                     LYISMLYGFDETIDIFLNALTTPIAQELLNKKIVMDILAMKTRDGELGLFAAMENNHP
                     LCVTRFLSKINGIAFKYKLSKANIMDLLKGATAHGTPVLYIAMSKGNEDVVLSYISTL
                     NIFAKKYSFSQRQLFTLLAAKNHDNMSAVHIAIHHNHYKTVETYYAAINVISQSLSFS
                     ADELKTYL"
     gene            2429..4357
                     /gene="thrS"
                     /locus_tag="ACHURJ_003208"
     CDS             2429..4357
                     /gene="thrS"
                     /locus_tag="ACHURJ_003208"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFR1282044.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHAARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            4361..4903
                     /gene="infC"
                     /locus_tag="ACHURJ_003209"
     CDS             4361..4903
                     /gene="infC"
                     /locus_tag="ACHURJ_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFR1282045.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQRVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            5000..5197
                     /gene="rpmI"
                     /locus_tag="ACHURJ_003210"
     CDS             5000..5197
                     /gene="rpmI"
                     /locus_tag="ACHURJ_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFR1282046.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            5250..5606
                     /gene="rplT"
                     /locus_tag="ACHURJ_003211"
     CDS             5250..5606
                     /gene="rplT"
                     /locus_tag="ACHURJ_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFR1282047.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    5723..5847
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            5729..5773
                     /gene="pheM"
                     /locus_tag="ACHURJ_003212"
     CDS             5729..5773
                     /gene="pheM"
                     /locus_tag="ACHURJ_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFR1282048.1"
                     /translation="MNAAIFRFFFYFST"
     gene            6056..7039
                     /gene="pheS"
                     /locus_tag="ACHURJ_003213"
     CDS             6056..7039
                     /gene="pheS"
                     /locus_tag="ACHURJ_003213"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFR1282049.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            7054..9441
                     /gene="pheT"
                     /locus_tag="ACHURJ_003214"
     CDS             7054..9441
                     /gene="pheT"
                     /locus_tag="ACHURJ_003214"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFR1282050.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDCLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            9446..9745
                     /gene="ihfA"
                     /locus_tag="ACHURJ_003215"
     CDS             9446..9745
                     /gene="ihfA"
                     /locus_tag="ACHURJ_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFR1282051.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
BASE COUNT         2605 a         2195 c         2503 g         2477 t
ORIGIN      
        1 atagttggtt ataaatacgt ctttagttca tcagcactaa aactcaggct ttggctgatt
       61 acatttatag cagcataata tgtttctaca gttttataat gattatgatg aatggctata
      121 tgaacagctg acatgttgtc atgattttta gcggccaaca gtgtgaataa ctgacgttga
      181 ctaaaagaat atttttttgc aaaaatattc agcgtcgata tataagataa cacgacgtct
      241 tcattaccct tgctcatggc gatgtataat acaggggttc catgtgctgt agcgcctttt
      301 aataaatcca tgatgttagc tttgctcaac ttgtatttaa aggcgatgcc attaatttta
      361 gagaggaacc gtgtgacaca caaagggtga ttattttcca ttgcagcaaa caaccctagt
      421 tcaccatcac gtgttttcat tgctaaaata tccatcacta tttttttgtt taaaagctct
      481 tgtgctattg gagtggttaa tgcattcaga aagatatcga ttgtttcatc gaacccatac
      541 aacatggaga tatagagccc aggtgtttcg ttagcacttt tagtttgcag taatttaacc
      601 agattatctt catgaatgat atgattgtat atcagtgact gaatttcttg cataaatatt
      661 tttacgttgt cagtatgtcc gttcatcata gccagaaata aagcaggtgt accaacgcta
      721 ttctgcgcag ttattatttc aatgatttct ttttcaggga gtatgggcaa ctgtttgaat
      781 aatgtaataa gctgttgagt ctggcctgtg gccattatta acaacattgc cgatggatgt
      841 ataggggcaa atggcattcg tgatgcaata cccgcgaaaa tagcccctgg aactcccaca
      901 ggtaataggg gcagaatagc aattgcacag ttgcgaacat acctatcagc ccaggtctga
      961 gcatcgggac ctgaactgat gaacttatca agcgaaagaa agccccgtgc tgtcctgtgg
     1021 gtgcctctga cggcaacgtt ggtatcgttc gggtcgtaga cggacactac atagtttaat
     1081 tgtccataca ctaactgctt tattcgtagc cttactgaca aagtatgatt gtctatatat
     1141 aaaatcgcgg acatatgact gacattgttt gccatcattc gacaaaagaa aatatctaaa
     1201 gcgaagccaa aatccttact cgcgataacg cgactccctg caggaggatg gcgaagtaat
     1261 gctctgaact ccgcatcccg gactaagggg acatgctgct gtatggattc aacggaggag
     1321 taatgtttaa tcaggttgac tttgcctttt ccatttctca atgaatcaag ggcgtattgc
     1381 aatgacagat ggcgacaaaa aatagcgtca gaaggagatt gcaaaaaaca tgcactacca
     1441 ttgagattta ctgttggttg tatagttttt tctctatgga tttttttaga cagtttcata
     1501 caatggttac gatcttcggc agttaactgt tcctgctcaa tataatccag tatgcttcgc
     1561 aaatggttag ccttaatagg cgtggtttcc agaaataagg gaatgatttc ttttttatag
     1621 tgtaatgtaa taagtatatt ttctgcatgg atgggtgaag tggcaaagtt aatgataaaa
     1681 agggcagtgg agtaacaatt ttgtgatatt tttcgacagt ttgcggtaaa ttcctgagta
     1741 atacagtatg acagcatcga acttaagtca gaggtatcaa gtagggtgat gagttggcgc
     1801 gcaaattgat taataagctc tgatgaaggc gttggtatgt cgctatcgat gtgcaatcta
     1861 tgaggagact tataatgagt tctgataatg atatcgtttt gactcataat ctattcacct
     1921 tatatatact ggaggttttg aggatgatga ttaattatta tcgccagaac gaaatgtgca
     1981 cgaaaaattg ctataagaat tgtgtcactt ttatcattgt gatatgacca cttcgtcatt
     2041 aatctcatga tcgtttaaat actaccgcca aatcacgcaa cgaactttgc tggattcagt
     2101 ttatgctgct gtaaatccgc tcgcagtaaa cctttcagac gcaccgtgat gttatcagtg
     2161 gttctttaag cgttttgctg gtgtactcac tacaaacgaa ttgcgaatcg aatcaatgtg
     2221 aaacggaaag gtacaatctc ccttttttta gtgttaacat cgttcaaccg ggtggaaggt
     2281 gagaggttaa tgtctttcat ttgtgtacct gattgcgaac caatttagca tttgttggct
     2341 agatggtttc gcaatgaact gttaataaac aaatttttct ttgtatgtga tctttcgtgt
     2401 gggtcaccac tgcaaataag gatataaaat gcctgttata actcttcctg atggcagcca
     2461 acgccattac gaccacgctg taagccccat ggatgttgcg ctggacattg gtccaggtct
     2521 ggcgaaagcc tgtatcgcag ggcgcgttaa tggcgaactg gttgatgctt gcgatctgat
     2581 tgaaaacgac gcacaactgt cgatcattac cgccaaagac gaagaaggtc tggagatcat
     2641 tcgtcactcc tgtgcgcacc tgttagggca tgcgattaaa caactttggc cgcataccaa
     2701 aatggcaatc ggcccggtta ttgacaacgg tttttattac gacgttgatc ttgaccgcac
     2761 gttaacccag gaagatgtcg aagcactcga gaagcggatg catgagcttg ctgagaaaaa
     2821 ctacgacgtc attaagaaga aagtcagctg gcacgcagcg cgtgaaactt tcgccaaccg
     2881 tggggagagc tacaaagtct ccattcttga cgaaaacatc gcccatgatg acaagccagg
     2941 tctgtacttc catgaagaat atgtcgatat gtgccgcggt ccgcacgtac cgaacatgcg
     3001 tttctgccat catttcaaac taatgaaaac ggcaggggct tactggcgtg gcgacagcaa
     3061 caacaaaatg ttgcaacgta tttacggtac ggcgtgggca gacaaaaaag cacttaacgc
     3121 ttacctgcag cgcctggaag aagccgcgaa acgcgaccac cgtaaaatcg gtaaacagct
     3181 cgacctgtac catatgcagg aagaagcgcc gggtatggta ttctggcaca acgacggctg
     3241 gaccatcttc cgtgaactgg aagtgtttgt tcgttctaaa ctgaaagagt accagtatca
     3301 ggaagttaaa ggtccgttca tgatggaccg tgtcctgtgg gaaaaaaccg gtcactggga
     3361 caactacaaa gatgcaatgt tcaccacatc ttctgagaac cgtgaatact gcattaagcc
     3421 gatgaactgc ccgggtcacg tacaaatttt caaccagggg ctgaagtctt atcgcgatct
     3481 gccgctgcgt atggccgagt ttggtagctg ccaccgtaac gagccgtcag gttcgctgca
     3541 tggcctgatg cgcgtgcgtg gatttaccca agatgacgcg catatcttct gtactgaaga
     3601 acaaattcgc gatgaagtta acggatgtat ccgtttagtc tatgatatgt acagcacttt
     3661 tggcttcgag aagatcgtcg tcaaactctc cactcgtcct gaaaaacgta ttggcagcga
     3721 cgaaatgtgg gatcgtgctg aggcggacct ggcggttgcg ctggaagaaa acaacatccc
     3781 gtttgaatat caactgggtg aaggcgcttt ctacggtccg aaaattgaat ttaccctgta
     3841 tgactgcctc gatcgtgcat ggcagtgcgg tacagtacag ctggacttct ccttgccgtc
     3901 tcgtctgagc gcctcctatg tgggcgaaga caacgagcgt aaggtaccgg taatgattca
     3961 ccgcgcaatt ctggggtcga tggaacgttt catcggtatc ctgaccgaag aattcgctgg
     4021 tttcttcccg acctggcttg cgccagttca ggttgttatc atgaatatta ccgattcaca
     4081 gtctgaatac gttaacgaat tgacgcaaaa actatcaaat gcgggcattc gtgttaaagc
     4141 agacttgaga aatgagaaga ttggctttaa aatccgcgag cacactttgc gtcgcgtccc
     4201 atatatgctg gtctgtggtg ataaagaggt ggaatcaggc aaagttgccg ttcgcacccg
     4261 ccgtggtaaa gacctgggaa gcatggacgt aaatgaagtg atcgagaagt tgcaacaaga
     4321 gattcgcagc cgcagtctta aacaattgga ggaataaggt attaaaggcg gaaaacgagt
     4381 tcaaacggcg cgccctaacc gtatcaatgg cgaaattcgc gcccaggaag ttcgcttaac
     4441 aggtctggaa ggcgagcagc ttggtattgt gagtctgaga gaagctctgg agaaagcaga
     4501 agaagccgga gtagacttag tcgagatcag ccctaacgcc gagccgccgg tttgtcgtat
     4561 aatggattac ggcaaattcc tctatgaaaa gagcaagtct tctaaggaac agaagaaaaa
     4621 gcaaagagtt atccaggtta aggaaattaa attccgtcct ggtacagatg aaggcgacta
     4681 tcaggtaaaa ctccgcagcc tgattcgctt tctcgaagag ggtgataaag ccaaaatcac
     4741 gctgcgtttc cgcggtcgtg agatggcgca ccagcaaatc ggtatggaag tgcttaatcg
     4801 cgtgaaagac gatttgcaag aactggcagt ggtcgaatcc ttcccaacga agatcgaagg
     4861 ccgccagatg atcatggtgc tcgctcctaa gaagaaacag taaggccttc aagtagcact
     4921 gtctgtggag ccttcgggct tcacaggctt tgttcgcctt tgtttcgttt attaacaatg
     4981 cgaagtggaa gttattaaaa tgccaaaaat taagaccgta cgcggtgctg ctaagcgctt
     5041 caaaaaaacc ggtaaaggtg gttttaagca caagcacgct aacctgcgtc acattctgac
     5101 caaaaaagcg accaaacgta aacgtcacct gcgtccgaaa gccatggttt ccaaaggcga
     5161 tctgggcctg gtaatcgcgt gcctgccgta cgcataagcc gttaacgttt ttaacttttt
     5221 aattagaata tagatacagg agagcacata tggctcgcgt aaaacgtggt gttattgcac
     5281 gcgcacgtca caagaaaatt ttgaaacaag ctaaaggcta ctacggtgcg cgttctcgcg
     5341 tataccgcgt tgccttccag gctgttatca aagctggtca gtatgcttac cgtgaccgtc
     5401 gtcaacgtaa gcgtcagttc cgtcaactgt ggattgcacg tatcaacgca gcagcacgtc
     5461 agaacggtat ttcttacagc aaattcatca atggcctgaa aaaagcctct gttgaaatcg
     5521 accgtaagat cctggctgat atcgcagtat tcgacaaagt agcgttcacc gctctggttg
     5581 aaaaagcgaa agcagctctg gcataagcca gttgaaagag ggagctagtc tccctctttt
     5641 cgtttcaacg ccatcaaaac attgactttt atcgccgtag ccttttcaat aaaggtcttt
     5701 tgaagagtac caaaaggtaa cgcaagcaat gaatgctgct attttccgct tcttttttta
     5761 ctttagcacc tgaatccagg aggctagcgc gtgagaagag aaacggaaaa cagcgcctga
     5821 aagcctccca gtggaggctt tttttgtatg cgcgttttga aaaataaaaa gccagtctat
     5881 caggcgtaat tgtcgcagcc agtctggata cggccagcgc ggagaaaccg gagcgtattt
     5941 aagtacgtga gaatttcgag cactgcccgg gaccaaaatg gcaagtaaaa tagcctgatg
     6001 ggataggctc taagtccaac gaaccagtgt caccactgac acaatgagga aaaccatgtc
     6061 acatctcgca gaactggttg ccagtgcgaa ggcggccatt agccaggcgt cagatgttgc
     6121 cgcgttagat aatgtgcgcg tcgaatattt gggtaaaaaa gggcacttaa cccttcagat
     6181 gacgaccctg cgtgagctgc cgccagaaga gcgtccggca gctggtgcgg ttatcaacga
     6241 agcgaaagag caggttcagc aggcgctgaa tgcgcgtaaa gcggaactgg aaagcgcagc
     6301 gctgaatgcg cgtctggcgg cggaaacgat tgatgtctcc ctgccaggtc gtcgcattga
     6361 aaacggcggt ctgcatccgg ttacccgtac catcgaccgt atcgaaagtt tcttcggtga
     6421 gcttggcttt accgtggcaa ccgggccgga aatcgaagac gattatcata acttcgatgc
     6481 tctgaacatt cctggtcacc acccggcgcg cgctgaccac gacactttct ggtttgacac
     6541 tacccgcctg ctgcgtaccc agacctctgg cgtacagatc cgcaccatga aagcccagca
     6601 gccaccgatt cgtatcatcg cgcctggccg cgtttatcgt aacgactacg accagactca
     6661 cacgccgatg ttccatcaga tggaaggtct gattgttgat accaacatca gctttaccaa
     6721 cctgaaaggc actctgcacg atttcctgcg taacttcttt gaggaagatt tgcagattcg
     6781 cttccgtcct tcctacttcc cgttcaccga gccttctgca gaagttgacg tcatgggtaa
     6841 aaacggtaaa tggctggaag tgctgggttg cgggatggtg catccgaacg tgctgcgtaa
     6901 cgttggcatc gacccggaag tttactctgg tttcgccttc gggatgggga tggagcgtct
     6961 gactatgttg cgttacggcg tcaccgacct gcgttcattc ttcgaaaacg atctgcgttt
     7021 cctcaaacag tttaaataag gcaggaatag attatgaaat tcagtgaact gtggttacgc
     7081 gaatgggtga acccggcgat tgatagcgat gcgctggcga atcaaatcac tatggcgggc
     7141 ctggaagttg acggtgtaga accggttgcc ggtagcttcc acggcgtggt cgttggtgaa
     7201 gtggttgagt gtgcgcagca tccgaacgct gacaaactgc gtgtgacaaa agtgaatgtc
     7261 ggcggcgatc gcctgctgga catcgtctgt ggtgcgccaa actgccgtca gggcctgcgt
     7321 gtggcggtag ctaccattgg tgctgttctg ccgggtgatt tcaaaattaa agcggcgaaa
     7381 ctgcgtggcg aaccgtctga agggatgctg tgctccttct ctgaactggg aatttctgac
     7441 gatcacagcg gcattatcga actgcctgca gatgcgccga ttggcactga catccgcgaa
     7501 tacctgaaac tcgatgacaa caccatcgaa atcagcgtaa cgccaaaccg tgccgactgc
     7561 ttaggtatca ttggtgttgc gcgtgacgtt gccgtgctga atcagctgcc gctggttgaa
     7621 ccggaaatcg ttccggttgg tgcgaccatc gacgacacgt tgccgattac agtcgaagcg
     7681 cctgaagcct gcccgcgcta tcttggccgt gtggtaaaag gcattaacgt taaagcgcca
     7741 actccgctgt ggatgaaaga aaaactgcgt cgttgcggga tccgttctat cgatgcagtt
     7801 gttgacgtca ccaactatgt actgctcgaa ctgggccagc cgatgcacgc tttcgataaa
     7861 gatcgcattg aaggcggcat tgtggtgcgg atggcaaaag agggcgaaac gctggtgctg
     7921 cttgatggca ctgaagcgaa gctgaatgct gacactctgg tcatcgccga ccacaacaag
     7981 gcgctggcga tgggcggcat ctttggtggc gaacactctg gcgtgaatga cgaaacgcaa
     8041 aacgtcttgc tggaatgcgc tttcttcagc ccgctgtcta tcaccggtcg tgctcgtcgt
     8101 catggcctgc atactgatgc gtctcaccgt tatgagcgtg gcgttgatcc ggcactgcag
     8161 cacaaagcga tggaacgtgc gacccgtctg ctgatcgaca tctgcggtgg tgaggctggt
     8221 ccggtaattg atatcaccaa cgaagcaacg ctgccgaagc gtgcaaccat cactttacgt
     8281 cgtagcaaac tggattgcct gatcggccat catattgctg atgaacaggt aactgacatt
     8341 ctgcgtcgtc tcggctgcga agtgaccgaa ggcaaagacg agtggcaggc agttgcgccg
     8401 agctggcgtt tcgatatgga gattgaagaa gatctggtcg aagaagtcgc gcgtgtttac
     8461 ggctacaaca acatcccgga tgagccggta caggcaagcc tgattatggg tactcaccgt
     8521 gaagctgacc tgtcgctcaa gcgcgtgaaa acgctgctca acgacaaagg ctatcaggaa
     8581 gtgatcacct acagcttcgt tgatccgaaa gtgcagcaga tgatccatcc aggcgttgaa
     8641 gccttactgc tgccaagccc gatctctgtt gaaatgtcag caatgcgtct ttctctgtgg
     8701 accggcctgc tggcaaccgt ggtgtacaac cagaaccgtc agcagaaccg tgtgcgcatt
     8761 ttcgaaagcg gtctgcgttt cgtaccagat actcaggcac cgttgggcat tcgtcaggat
     8821 ctgatgttag ccggtgtgat ttgcggtaac cgttacgaag agcactggaa cctggcaaaa
     8881 gagaccgttg atttctatga tttgaaaggc gatcttgaat ccgttctcga cctgaccggt
     8941 aaactgaatg aggttgagtt ccgtgcagaa gcgaatccgg cactgcatcc ggggcaatcc
     9001 gcagcgattt atctgaaagg ggaacgtatt ggttttgttg gagttgttca tcctgaactg
     9061 gaacgtaaac tggatcttaa cggtcgcact ctggtgttcg aactggagtg gaacaagctc
     9121 gcagaccgcg tggtgcctca ggcgcgcgag atttctcgct tcccggcgaa ccgtcgtgac
     9181 atcgcggtgg tggtcgcaga aaacgttccc gcagcggata ttttatccga atgtaagaaa
     9241 gttggcgtaa atcaggtagt tggcgtaaac ttatttgacg tgtaccgcgg taagggtgtt
     9301 gcggaggggt ataagagcct cgccataagc ctgatcctgc aagataccag ccgtacactc
     9361 gaagaagagg agattgccgc taccgtcgcc aaatgtgtag aggcattaaa agagcgattc
     9421 caggcatcat tgagggattg aacctatggc gcttacaaaa gctgaaatgt cagaatatct
     9481 gtttgataag cttgggctta gcaagcggga tgccaaagaa ctggttgaac tgtttttcga
     9541 agagatccgt cgcgctctgg aaaacggcga acaggtgaaa ctctctggtt ttggtaactt
     9601 cgatctgcgt gataagaatc aacgcccggg acgtaacccg aaaacgggcg aggatattcc
     9661 cattacagca cggcgcgtgg tgaccttcag acccgggcag aagttaaaaa gccgggtcga
     9721 aaacgcttcg cccaaagacg agtaatctga tctaactaaa aaggccgctc tgcggccttt
//
LOCUS       DAZPEB010000154         9657 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZPEB010000154 DAZPEB010000000
VERSION     DAZPEB010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9657
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.157"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(1..1093)
                     /gene="fdeC"
                     /locus_tag="ACHURJ_003216"
                     /pseudo
     CDS             complement(1..1093)
                     /gene="fdeC"
                     /locus_tag="ACHURJ_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308363.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin FdeC"
     gene            1646..1735
                     /locus_tag="ACHURJ_003217"
                     /pseudo
     CDS             1646..1735
                     /locus_tag="ACHURJ_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2209..2310
                     /gene="ykgR"
                     /locus_tag="ACHURJ_003218"
     CDS             2209..2310
                     /gene="ykgR"
                     /locus_tag="ACHURJ_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YkgR"
                     /protein_id="HFR1282052.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY"
     gene            2674..2937
                     /locus_tag="ACHURJ_003219"
     CDS             2674..2937
                     /locus_tag="ACHURJ_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFR1282053.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGS"
     gene            2937..3077
                     /gene="ykgO"
                     /locus_tag="ACHURJ_003220"
     CDS             2937..3077
                     /gene="ykgO"
                     /locus_tag="ACHURJ_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFR1282054.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(3112..3339)
                     /gene="ykgL"
                     /locus_tag="ACHURJ_003221"
                     /pseudo
     CDS             complement(3112..3339)
                     /gene="ykgL"
                     /locus_tag="ACHURJ_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgL"
     gene            4163..4705
                     /gene="ecpR"
                     /locus_tag="ACHURJ_003222"
                     /gene_synonym="matA"
     CDS             4163..4705
                     /gene="ecpR"
                     /locus_tag="ACHURJ_003222"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HFR1282055.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLSEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMQPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKIYKLVQ"
     gene            4731..5366
                     /gene="ecpA"
                     /locus_tag="ACHURJ_003223"
                     /gene_synonym="matB"
     CDS             4731..5366
                     /gene="ecpA"
                     /locus_tag="ACHURJ_003223"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004965403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HFR1282056.1"
                     /translation="MTHWDITSSIQLGKKCNEKKVLAIALVTVFTGMGVAQAADVTAQ
                     AVATWSATAKKDTTSNLVVTSLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKL
                     TSRLITNTLTQLDTSGSTLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYN
                     ASNRTTAQDGFTFTIISGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            5424..6092
                     /gene="ecpB"
                     /locus_tag="ACHURJ_003224"
                     /gene_synonym="yagY"
     CDS             5424..6092
                     /gene="ecpB"
                     /locus_tag="ACHURJ_003224"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HFR1282057.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            6118..8642
                     /gene="ecpC"
                     /locus_tag="ACHURJ_003225"
                     /gene_synonym="yagX"
                     /pseudo
     CDS             6118..8642
                     /gene="ecpC"
                     /locus_tag="ACHURJ_003225"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414825.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
     gene            8632..>9657
                     /gene="ecpD"
                     /locus_tag="ACHURJ_003226"
     CDS             8632..>9657
                     /gene="ecpD"
                     /locus_tag="ACHURJ_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HFR1282058.1"
                     /translation="MRVNLLITMIIFALIWPVTALRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYTGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYMQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSLSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLG"
BASE COUNT         2629 a         2369 c         2257 g         2402 t
ORIGIN      
        1 caatacggat cacttcagat ttgcggtact caagaacgat gttgttatta cgctcaacca
       61 ggtcatagcg gctgcctgcc agcattcgac gctcgcgaat gctgtctgta tcgagttgtt
      121 tctccagagg ttcgccaatc cgataattaa cttccaggcc aaagcgagtg tcattctcac
      181 cactcttgcc ctgcttatgc ccggcactca gggtcagaag aggcacaggc gtgtaattca
      241 cttcagcgga aatcgcatgt ggatctttct ggcgtttatc tttgccaaac agcccaactt
      301 catcgccata atactgctca tacatcaggc ttgcgccaag ctgcggtcag gcgggtaaat
      361 agccctcagc acgaatatcc cagccattcg ccgggcgttc ctgataatcc tcaatatccg
      421 gcgatttttt ccagccagaa gcccgaatat aaccattggc gctcagtttc agataatcgc
      481 gccagtattc cgcaccaaca ccaatgcggg tatgactacg ggataaatca tggtcgataa
      541 aggtgttcac cccccccatt cagtcatttc ctgaaaaatg acgccagcca aaaccaatat
      601 ttgactgagt acgatcgtct gtacgatgta ttgccccctg agtgaacaac atatttgtcg
      661 gcgtatcata aatcggataa agcatttcca gcgaagaatc cttcagcgag aaatctttat
      721 cgacattcag tttgacgcgc gctgtaccat atttcccgag ccactcctgt atttcctggt
      781 tagctttagc ggtggccatt ccggtaataa aattacgtgt cgcatcgcta tctggctgac
      841 tgcttaaaaa tgtcccggca tttgcggcaa acgacgcgac atttttctcc acgttattat
      901 cagcagttac cgtagtattt cccatgctca accgtggctg aaccgcatgc tgcgcacgtg
      961 ccgccattac tggggtaaag gtgacagcga gtggaaaaag aacctgaaca gagatatttg
     1021 cccacgccac gcagcgggcc agaactgaat aacgaaatcg tggttgttta tgacctgttt
     1081 tataacgtga cattgagata atttcctgat gaacgaatta acgaacatag gcagacacaa
     1141 ataatggcgt ctgcttgtca gaaaaaccgg ggatttcccg gtttcctttt ccgcagaaaa
     1201 ggaaatacct ttatgtaaga aagtgcaccg ccacctgata tatccagtgt ctgtagccac
     1261 tcatcaggtg gttgggcgcg ggattatatt tttatttatg ggtaaaaatt tagctttttg
     1321 tgcaagtcat taaatagata cctaagttat atttcttact ctgcggacag tgaataattc
     1381 attattcaga ctgaataata atgacctgcc tttaattagc aactattgga aatacgtcga
     1441 agtaggtata tacgccagga taaaccgata tatttattat ggatattcat tttccttcat
     1501 cggactgtaa attgcgggga tatcgaactt acactactca tcgcaacact caaacagatt
     1561 tatgaatttc gttcataacc cctgacaaat cctgccgtct aatcgagctt ttccctttac
     1621 gttatgcttc tcatgaacga gaacacaact tattcatgaa ttaaccattc cggataaact
     1681 atggaatcaa attcaggcat cgaaaggaac tggctggttt ccgttaatag attaaatatc
     1741 tattactctc ccgagagtaa aactatcaaa tcaatgagta accgatacag cctcacaaat
     1801 tctttcagat aattaacccg tcactgcggc acgttctctc gtaaggcgtg cctgcagtgt
     1861 aatccagtat gtatccggca tagttgacgg tttaatagaa ctcaattcct ctccctggtc
     1921 accaacaacg atatcttgta taacacccca ggagatacga cattatgatg agatgcgata
     1981 tgctcctcag caccaatcaa cgccaggact gggccagtct tgagatcaga ctcattcccg
     2041 ggactgactt cagtcgacat atatcaatga cgttggtgca ataaaagata gccgatgtaa
     2101 tacatctatt caaatttgtg atacatgtca aaaaactggt tgaccaaact cgttatttat
     2161 ataagggcac ttacgaagtg cgctcttttt taaagcgagg aagtaccaat gaaagagaat
     2221 aaagtacaac aaatcagtca taaactaatt aatatcgttg tttttgtcgc aattgtagaa
     2281 tacgcctatt tatttctcca tttctattaa taacggcaat aaactgttca cttcagtgat
     2341 atttaaaata tgcattctct ccctttttgt aagtaattat tatatccgtg ggagaggaat
     2401 acacgctgtc aggtaatcaa tcatactgcg ataaatcatc ggccagtaaa gtggagataa
     2461 cctccattgt cgaaaaatcc atactctcag cgaaaccatc atcaatcact catccaggcg
     2521 tttatgggag cgtcgccaac ggctgttaac aatgccagac ttccccgttg cggaaattcc
     2581 acataccact aatggtcaca gggattgggt gttgaaatga tccggatgag catgtatctt
     2641 tatggttatg ctataacata acaggtaaaa atgatgaagc ccaatatcca tcctgagtat
     2701 cgtactgtgg tgttccacga caccagtgtt gatgagtact ttaaaatcgg ctcgactatc
     2761 aaaacagacc gtgagattga gctggatggc gtaacgtatc cgtacgtgac aattgatgtc
     2821 tcttctaaat cgcacccgtt ctatacaggg aagctgagaa cagtggcatc agaaggaaat
     2881 gttgcacgat tcacccaacg ttttggtcgt tttgttagca cgaaaaaggg gtcgtgatga
     2941 aagttcttaa ctctctgcgt accgcaaaag aacgccatcc agactgtcag attgtgaagc
     3001 gaaaaggacg gctatatgtg atttgtaaat ctaatccacg ttttaaggcc gttcaggggc
     3061 gtaagaaaaa acgttgattc aaaattcgac ggattaacga tatttgtctg attaataatc
     3121 agatcggatt aatgttggtg tgtttataac accaacatta attttcctgg ggatatattc
     3181 ttcctgttca tttgaggcca actgcctgac gtttctctac gaatattcca ttatcttaat
     3241 gttgacttgt tgaccagctt cgcccctgta tgctggcatc aactctcttt tagactgaac
     3301 acgccactca gtctcctccc tttgcggcgc agcctgcatt ttcactcaaa ctgttaagat
     3361 gataaatgtg gtaaatctgt tggtactaac ataaaaacgt ttacgccaca ggaacagtct
     3421 gatccaccgg taaccccgtc gccgacgttc gagtgccagt tagagtaacg cgcacagata
     3481 actgcactca gtgccctgac aaagaggcca tcgttcctgt gacagctggc agccttcgtt
     3541 taacttcact taatctggct cttgagggct taccgaacag atgacgtaca tacgcccgtt
     3601 caattttcca tcacttattg taatgaacac ctataaccat tttgtgcggc atgttaatcc
     3661 attaaaattt cttactgatt ggcaaatcat cttcaatgac agctcattat agttttatat
     3721 tctatccctt acccttaaaa cttgtttttt tactagtcca tcacacagcg cattaagact
     3781 attcctaaca cttcagggca aagttcctga ccaatataaa atgcaagtaa gaattgaacg
     3841 ttatattgcc aataacctta tgaaaccaaa tgtctttttc ttcttatcaa aaaagcaata
     3901 ttttcagttt ttctaaatat tgacttaacc attgaattcc ttttccgttc acatattgac
     3961 actcatcgaa aaaaaaaaca taaatttaag cccaatcgaa aataattaaa cttaatctcg
     4021 tttaaccttt attgatatgt actacgtatc ttatttactt ccggtttact aaggaaactg
     4081 aatgcacctg taaaaattac aggtttggaa agtagtgaca tggcaaagtg attacagtag
     4141 ggactatgag gttaaaaacc atatggaatg tcaaaaccgt tctgataaat acatctggtc
     4201 tccccatgac gcctacttct ataaaggact atctgaactg attgtggata tcgacagatt
     4261 aatttatctg tcgttggaga aaattagaaa agatttcgtg tttatcaatc tcaatacgga
     4321 ttctttatct gaatttataa accgtgataa tgaatggtta tccgcggtaa aggggaaaca
     4381 ggtcgtattg attgcggcca gaaagtcaga agccttagca aattattggt actacaacag
     4441 caatattagg ggcgtggtat acgctggact gagtcgtgat attagaaaag aactggccta
     4501 tgtgattaat ggcaggttcc tgagaaaaga tattaagaaa gataaaatca cggaccggga
     4561 aatggaaatt atccgcatga cggcccaggg aatgcaacct aaatcgattg ccagaattga
     4621 aaattgtagt gtgaagacag tgtataccca tcggcgtaat gctgaggcca agctgtactc
     4681 aaaaatatat aagttggttc agtaaactcc aggcaagtta gttttaaaaa atgactcact
     4741 gggacatcac gtcctcaatt caactcggga agaaatgcaa tgaaaaaaag gttctggcaa
     4801 tagctctggt aacggtgttt accggcatgg gtgtggcgca ggctgctgac gtaacagctc
     4861 aggctgtagc gacctggtcg gcaacagcca aaaaagacac caccagtaat ctggttgtga
     4921 cgtcactcgg tagcttggcg ttccagtatg ccgaaggcat taaaggtttt aactcacaga
     4981 aaggtctatt tgacgtggct atcgagggtg actcaacggc taccgccttt aaactgacct
     5041 cacgtcttat caccaacaca ttaactcagt tggatacctc aggttccaca ctgaatgtgg
     5101 gcgtggatta taacggcgcg gcagtcgaaa aaactggcga taccgtgatg atcgataccg
     5161 ccaacggcgt actgggcggc aaccttagcc cgctggctaa cggttacaat gccagcaatc
     5221 gtaccaccgc acaggatggt ttcaccttca ccatcatcag cggcaccacc aatggtacca
     5281 ccgcagtaac cgattacagc actctaccgg aaggcatctg gagcggcgac gttagcgtac
     5341 agttcgacgc gacctggacc agttaatctc tctgatgtac cagcaggggt agcccccctg
     5401 ctttattcct ggacggaatt gttatgaaaa agcaccttct gcctctcgct ctgctgtttt
     5461 ccggaatatc tccggcccag gcgctggatg tcggcgatat atcatcgttt atgaacagtg
     5521 acagcagcac gctgagcaaa acgatcaaaa acagtaccga cagtggtcgc cttatcaata
     5581 tccgtctcga acggctctct tcaccgcttg acgacgggca ggttatctca atggacaagc
     5641 cggatgagtt gctactcact cccgccagct tgctgctacc cgcccaagcc agcgaagtga
     5701 tccgcttctt ctataaggga cccgcagatg aaaaagagcg ctactaccgc attgtctggt
     5761 ttgatcaggc cctcagtgat gcgcagcgcg ataatgccaa ccgcagcgct gtggccactg
     5821 cttccgcccg catcggcacc attctggtcg tcgcccctcg tcaggcgaac taccactttc
     5881 agtacgccaa cggctccctg acaaatacag gaaatgcgac gctgcggatc ctcgcctacg
     5941 gcccctgcct gaaagccgcc aacggtaagg agtgtaaaga gaattactac ctgatgccgg
     6001 gcaagtcgcg tcgttttacc cgcgtggaca ctgcggataa caaaggacgg gttgcacttt
     6061 ggcagggtga taagttcatt cccgtgaaat agatagctgt gcagatggat aacgacaatg
     6121 cctttacgac ggttctcccc aggactgaaa gcccagtttg ccttcggcat ggtctttttg
     6181 ttcgttcagc ccgatgccag cgctgctgac ataagtgcgc agcaaatagg tggggtgatt
     6241 attccgcagg ccttcagtca ggcgcttcag gacggcatga gcgtcccgct ctatattcat
     6301 ctcgccggta gtcagggtcg ccaggacgat cagcgaatcg gcagcgcttt tatctggctg
     6361 gatgatggac agctacgcat ccggaaaata cagctggaag agagtgaaga taacgccagt
     6421 gtcagcgaac aaactcgaca gcagctgatg gctctggcga acgccccgtt caatgaggcc
     6481 cttaccatcc cctgactgac aacgcgcagt tggatctcag cttgcgccaa ctgctgctgc
     6541 agctggtggt caagcgcgaa gcgctgggca ccgtactacg ctcacgtagc gaagacatcg
     6601 ggcagtccag tgttaacacc ctcagcagta atctgagcta taacttgggc gtctataaca
     6661 accagttgcg taacggcggg agcaacacat ccagctatct gtcgctgaat aacgttactg
     6721 cgctgcgcga acatcatgtg gtgctcgacg gctcgctgta cgggatcggt agcggtcaac
     6781 aggacagtga attatataaa gcgatgtatg aacgcgattt tgccggtcac cgatttgccg
     6841 gtggaatgct cgacacctgg aacttgcagt cattagggcc gatgaccgcc atttcagcag
     6901 ggaagattta cggcctttcc tggggaaacc aggccagctc caccatcttc gacagcagcc
     6961 agtcagccac gccagtgatc gcctttttac cggcggcggg cgaagtacat ctcacccgtg
     7021 atgggcggct actaagcgtt cagaacttca ctatgggcaa tcatgaagtg gatacccggg
     7081 gtctaccgta cggtatttac gatgtggaag ttgaggtgat cgttaacggt cgcgtgatca
     7141 gcaaacgcac tcaacgggtc aataagctgt ttagccgggg cgcggcgtcg gtgcaccact
     7201 ggcgtggcag gtatggggcg gtagctttca tatggatcgc tggtcggaaa acgggaaaaa
     7261 gacgcgacca gctaaagaga gttggctagc aggtgcctcg acctccggct cattgagtac
     7321 gcttagctgg gcggcaacgg gatatggata cgataatcag gcggtgggtg aaacccgtct
     7381 gacgctgccg cttggggggg cgatcaacgt taacctgcaa aatatgctgg ccagtgacag
     7441 ctcatggagc agcatcggca gcatcagcgc tactctaccg ggaggcttta gttcgctgtg
     7501 ggttaaccag gaaaaaaccc gtattggcaa tcaattgcga cgtagcgatg ccgacaaccg
     7561 tgcaatcggc ggcacactca acctgaactc actgtggtcg aagctgggca cattcagcat
     7621 cagctacaat gatgaccgcc gttacaacag ccattattac acggcagatt actatcaaaa
     7681 tgtctacagc ggtacctttg gttcgcttgg cctgcgggcc ggtattcagc gctataacaa
     7741 cggcgacagc aacgccaata cagggaaata tatcgctctc gatctctcgc taccactggg
     7801 caactggttt agcgcaggga tgacccatca aaacggctac accatggcaa acctgtcagc
     7861 acgcaaacag tttgatgaag ggaccattcg cactgttggt gccaatctgt cacgtgccat
     7921 ctccggcgat accggtgatg acaaaaccct cagcggtggg gcgtatgcac agttcgacgc
     7981 tcgttacgcc agcggaacgc tgaacgtcaa tagcgcggcg gacggctacg tcaataccaa
     8041 cttgaccgcc aatggcagcg tcggctggca gggtaaaaac attgctgcca gcgggcggac
     8101 tgatggcaac gctggggtga tattcaacac cgggctggag gacgacggtc agatcagcgc
     8161 caaaatcaac gggcggattt tcccgcttaa cggcaagcgt aactatctcc cgctctctcc
     8221 ctatggaaga tatgaggtgg agttacagaa cagcaaaaac tcactcgaca gttacgatat
     8281 cgtcagcggc cgcaaaagtc gtctgactct ctatccaggc aatgtcgctg tcattgagcc
     8341 agaggtgaag cagatggtta ccgtctccgg tcgtatccgt gcggaagacg gcacactgct
     8401 ggctaacgca cggattaaca atccatatcg gccgaacccg aaccgatgaa aacggcgagt
     8461 ttgtcatgga cgtggataag aaatacccca ctatcgattt tcgctacagt ggcaataaaa
     8521 cctgcgaagt ggctctggaa ctcaaccaag cgcgcggtgc cgtctgggtc ggtgatgtgg
     8581 tctgcagcgg cctctcatcg tgggcggcgg tgacgcagac aggagaagag aatgagagtt
     8641 aacctactaa taacgatgat aattttcgcg ctaatctggc cagtaactgc gctcagagcg
     8701 gcagtgagca aaacaacctg ggcggatgca ccggcacgcg agtttgtgtt tgtcgaaaac
     8761 aactcagacg acaacttttt cgtcactcct ggcggggcgc tggatccgcg cctgaccggt
     8821 gccaaccgct ggaccggttt aaaatacact ggttcaggaa ccatctatca gcaaagcctc
     8881 ggctacattg ataacggtta caacaccggc ctttatacca actggaagtt tgatatgtgg
     8941 ctggaaaatt cgccggtttc atctccttta actggcttac gctgcatcaa ctggtacgca
     9001 gggtgtaata tgaccaccag tcttatcctg ccgcaaacca ccgacgccag tggattttat
     9061 ggcgcgacgg tgaccagcgg cggcgcgaag tggatgcacg gcatgttgtc agacgcgttt
     9121 taccagtata tgcaacaaat gcccgtcggc agcagcttta caatgaccat caatgcctgt
     9181 cagacctctg tgaactatga cgccagcagc ggcgcgcgtt gtaaggatca ggcctccggc
     9241 aactggtatg ttcgcaatgt cacccatacg aaagcagcaa atcttcggtt aataaatacc
     9301 cactcgctgg cggaggtatt tatcaacagc gacggggtgc cgaccctggg cgaagggaac
     9361 gccgactgcc ggacacagac catcggcagc ctttcaggat taagttgtaa gatggtcaac
     9421 tataccctgc aaacaaacgg actcagcaac acctcaatcc atatattccc ggcgatcgcc
     9481 aactcgtcgt tagcctcggc cgtcggggcg tacgatatgc agttcagtct gaatggcagt
     9541 tcatggaaac cggtgagcaa taccgcctat tactacacct tcaacgagat gaagagcgca
     9601 gactcgatct atgttttctt ctcgagcaac ttctttaagc agatggtgaa cctcggg
//
LOCUS       DAZPEB010000155         9498 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZPEB010000155 DAZPEB010000000
VERSION     DAZPEB010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9498
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.158"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..191
                     /locus_tag="ACHURJ_003227"
     CDS             <1..191
                     /locus_tag="ACHURJ_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFR1282059.1"
                     /translation="AHFMMSGGNILALQKILGHHDIKMTMRYAHLAPDHLETALRFNP
                     LATLPSGDKVAAAVGITP"
     gene            complement(296..832)
                     /gene="rcdA"
                     /locus_tag="ACHURJ_003228"
     CDS             complement(296..832)
                     /gene="rcdA"
                     /locus_tag="ACHURJ_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFR1282060.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            916..2124
                     /locus_tag="ACHURJ_003229"
     CDS             916..2124
                     /locus_tag="ACHURJ_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1282061.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            2124..2939
                     /gene="ybjI"
                     /locus_tag="ACHURJ_003230"
     CDS             2124..2939
                     /gene="ybjI"
                     /locus_tag="ACHURJ_003230"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFR1282062.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGNGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            3025..3309
                     /gene="ybjH"
                     /locus_tag="ACHURJ_003231"
     CDS             3025..3309
                     /gene="ybjH"
                     /locus_tag="ACHURJ_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFR1282063.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYARHLASVGEGMVIEHQIDDHHH"
     gene            complement(3350..4580)
                     /gene="mdfA"
                     /locus_tag="ACHURJ_003232"
                     /pseudo
     CDS             complement(3350..4580)
                     /gene="mdfA"
                     /locus_tag="ACHURJ_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
     gene            4865..5461
                     /gene="ybjG"
                     /locus_tag="ACHURJ_003233"
     CDS             4865..5461
                     /gene="ybjG"
                     /locus_tag="ACHURJ_003233"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFR1282064.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            5519..6277
                     /gene="deoR"
                     /locus_tag="ACHURJ_003234"
     CDS             5519..6277
                     /gene="deoR"
                     /locus_tag="ACHURJ_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFR1282065.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPHSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(6324..7515)
                     /gene="dacC"
                     /locus_tag="ACHURJ_003235"
                     /pseudo
     CDS             complement(6324..7515)
                     /gene="dacC"
                     /locus_tag="ACHURJ_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
     gene            7762..8387
                     /gene="gstB"
                     /locus_tag="ACHURJ_003236"
                     /pseudo
     CDS             7762..8387
                     /gene="gstB"
                     /locus_tag="ACHURJ_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
     gene            complement(8384..>9498)
                     /gene="yliI"
                     /locus_tag="ACHURJ_003237"
     CDS             complement(8384..>9498)
                     /gene="yliI"
                     /locus_tag="ACHURJ_003237"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFR1282066.1"
                     /translation="HRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLPD
                     NHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWLS
                     YSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYLF
                     IALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQGM
                     AMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAGT
                     EQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRIL
                     TDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
BASE COUNT         2296 a         2456 c         2342 g         2404 t
ORIGIN      
        1 cggcgcactt catgatgtcg ggtggaaaca ttctggcctt acaaaaaatt ctcggacacc
       61 acgatataaa aatgactatg cgttacgcac atctggcacc ggatcatctg gaaacggcgc
      121 tccgtttcaa tcctctggca acgctgccaa gtggcgacaa agtggcggca gcggttggca
      181 ttaccccgta ataaccacca ctgaccacca acctaactta ttgtttttaa tctaacttat
      241 tgttttcatt aacccgttta cataaatggg ttttttgtta tctaaaattt atctactacc
      301 ctgcaaccct ctcaaccatc ctcaaaatct cctcgcgcga tagcggctta cggtcggtga
      361 caaaatgcag cgtcatcccc tcaataaacg catcaagcgc gcgggcggtt ccgggttcaa
      421 accattgttc gagcgtttgc tgactgcgct gcatccagtt ttgcattacc gtttttaata
      481 gcggttttcg gctagccagc gcgtagagct ggtacatcag ctccatatta tccggcgttg
      541 caacctgtga gctgtagatc atatcggtga tagcctggca tgcgccttga gcatcactaa
      601 catcgctaaa aaatgcctga tattgccggg acataatctc agtaaaactg ctgaacgcct
      661 ccagcaacaa ctcatcaatt cctgaaaaat agtaggtcat cgatcccaac ggtaccccgg
      721 caagggtagc aattttgcgg tgcgtaacag catgtattcc gtaaagtttc accgcctcca
      781 gcgtggcctg gataattttt tctcgccgtt gcggatcgtt agcgcgacgc atgtcatttc
      841 ctctgtctgc aattgtgtac aaatgtacac aaccttgcta atgttgtctt ccctcttctt
      901 attttgacgt gatctatgac cgtaaattct tcacgtaatg cattgaaacg ccgaacctgg
      961 gcgctgttta tgttcttctt tttgccaggc ctgttaatgg cgtcctgggc aacccgtacg
     1021 cctgctatcc gcgatattct ctctgtctcg atcgctgaaa tgggcggtgt tctctttggt
     1081 ctgtcgatcg gttcgatgag cggtattctc tgctcggcgt ggttagtgaa acgctttggg
     1141 acacgtaatg tcatcctggt cacgatgtcc tgcgcattga tcgggatgat gatattaagt
     1201 ctggcactct ggctgacatc gcccctgctc tttgccgttg gtctcggcgt ctttggggca
     1261 agttttggtt ctgcggaagt ggcgataaac gttgaaggtg ccgccgttga gcgagaaatg
     1321 aataaaacgg ttttgccgat gatgcacggt ttttatagcc tgggcacgct ggcaggcgct
     1381 ggtgtcggga tggcactgac ggcctttggc gttccggcaa cggtgcacat tttattggcg
     1441 gcgctggtag gtatcgcgcc tatttatatc gccattcagg caatccctga cggtacgggc
     1501 aaaaatgctg ccgatggcac ccagcatggc gaaaaaggca ttccttttta tcgcgatatc
     1561 cagttgctgt tgattggtgt tgtagtgctg gcgatggcct ttgccgaagg ttctgccaac
     1621 gactggttac ccttattaat ggttgacggt cacggtttta gccctacttc cggctcgctg
     1681 atttatgccg gttttaccct ggggatgacc gttggacgct tcactggcgg ttggttcatc
     1741 gaccgttaca gtcgcgttgc cgtggttcgg gccagtgcac taatgggggc gttgggtatt
     1801 gggctgatta tttttgtcga tagcgcctgg gtcgctgggg tgtctgttgt actctgggga
     1861 ctgggtgcct cgctgggctt cccgctgacc atttctgccg ccagcgatac cggccccgat
     1921 gcaccaaccc gcgtcagtgt ggtagcaacg accggttatc tggctttcct cgtcgggccg
     1981 ccgctgctgg gctatctcgg cgaacattat ggattacgta gtgcaatgct ggttgtactg
     2041 gcgctggtta ttctcgcggc tattgtcgcg aaagccgtcg ccaaacccga taccaaaacg
     2101 cagacggcga tggagaatag ttgatgagca ttaaattaat tgcggtagac atggatggta
     2161 ctttcttaag cgatcaaaaa acctataacc gtgagcggtt tatggctcag tatcagcaaa
     2221 tgaaagcaca aggcattcgc tttgtggtcg ccagcgggaa tcaatattat cagttgatct
     2281 ctttcttccc tgaaattgct aatgaaattg cctttgtggc tgaaaacggc ggctgggtag
     2341 tgagtgaagg caaagatgtt tttaatggcg agctgtcgaa ggatgccttt actactgtcg
     2401 tggaacattt gctgacgcgc ccggaagtgg aaattattgc ctgcggaaaa aatagcgcct
     2461 atacgctcaa aaaatatgac gatgccatga aaacggtggc ggaaatgtat tatcaccgtc
     2521 tggaatacgt tgataacttt gacaacttag aagatatttt ctttaagttt ggcctgaatc
     2581 tttccgatga actgattcca caagtacaaa aagcattaca tgaggccatc ggcgatatta
     2641 tggtgccagt ccacaccggc aacggcagca tcgatctgat tatccccggc gtacataaag
     2701 ccaatggcct tcgccaactg cagaaattat ggggaataga cgacagcgaa gtggtggtct
     2761 ttggcaatgg cggtaacgat attgagatgc tgcgtcaggc aggctttagt tttgcaatgg
     2821 aaaatgccgg cagcgcggtc gtcgcagcgg caaaataccg ggcaggctcc aataaccgtg
     2881 aaggcgtact ggatgtgatc gataaagtgc ttaaacacga agcgccattt gaccaataaa
     2941 aacggcagca cgcccggcaa cacaatacct gaatgaaggc tgcataattc gcggaaatta
     3001 acccgctgca ttatttcagg cgaaatgatt atgaaaaatt gtctactgtt gggcgcgctt
     3061 ttaatgggct ttactggtgt ggcgatggcg caaagtgtca ccgtcgatgt gccgagtgga
     3121 tacaaagtgg ttgtagttcc tgattcagtc agcgttccac aagcggtcag cgtcgcgact
     3181 gtgccgcaaa ccgtctatgt ggctcctgcg ccagctccgg cttaccgccc gcatccttat
     3241 gctcgccatc ttgccagcgt aggtgaaggg atggttatcg aacaccagat tgacgaccat
     3301 caccattaat tcgagaattc ctgatcgcac aaagcagtca ggcatttttt tacccttcgt
     3361 gagaatttcc catctgttta tcttttaaaa agataaccat cagcgacagc cacaaaattc
     3421 cgttgacaag gttgaagaga ttaaactgtc cgttgccccc gttcagccag gcatgtttgc
     3481 tgatttcaat accaacggta aagatcagca tttgcagcat tcccatcgcc gcagaaaccg
     3541 tacctttact catatcgctg gcaaacaggg ttaatcgcac cagtcccgca ttcgccagac
     3601 caataccgaa agcataaata cttaacccgg cagtcatcca taaatacgcg tgcgatgaga
     3661 taaccgttgc cgcagcagcg accaacagac caatcataat cggccagccg cccataatta
     3721 tcagcgaacg tacggtgcgg cgcgaggtca gacgcgctaa cagcaagtta cccgcaatta
     3781 acgccccgaa aataggcact tgcagcaagc catattcata gctgctcaac tgctcgccgg
     3841 taatgatgat aatcggcgac tgggcgatcc acgccagcaa tggcaggcta acgaatccca
     3901 gcgccagcgc ccccgccaca aagcggccgt tcttcagcac cagcttatag tcacgaccga
     3961 gttctttcag tgacagtttc tcgcctatac gcgtggcggt ttcaggcata gctcgttgca
     4021 gaccgaaaaa ggagatcgct gccaatgcgg caaacaaaac aaacatcccc tcccagggca
     4081 gcacatggat ccacgccgcg cccaccagcg gaccaagtag cggagcaatc agcgccacgt
     4141 tcgccatcag cgcggtgatc ttgatacaaa ccgcctcttc gaaagattcc tgaattgcgg
     4201 cgtatcccac agcgccaatg aaacagaggc ttatgccctg caagaagcgc aacagagtga
     4261 attgttcaat gttttgcgcc agtaatattg ccagacaggt gatgataaac cacaccaccc
     4321 ccgccagcat caccggacgg cgaccaatac gatccgacag cggccccagg agccattgta
     4381 aaaacatccc gcccgccaga tatgcggtca ttgaagtagg aacccaatca atgcccgcct
     4441 gatattgttc caccacggcc aacataccgg gttgaatcat atctgccgat ataggttgaa
     4501 aattcgtaaa gcaccagaca gagagggaaa agtaacgcct gacgtccaag cctggcaccg
     4561 gaagctaatt tattttgcat gcaatttctt cgccaataat aatcgcgcag agtttaataa
     4621 aagcgcagct aacgagaaag cgaattttgt agctgaaacc acggttaagc acattcttac
     4681 attattgcga gtatagctac gctttcttta agttttattt aacctatgcc cgttacaatc
     4741 acccaccgta aacaggccgc ttgagggaaa taagacgatg ccgctttacc cagtttaacc
     4801 tgcactttat tctcaacgac ttgcctgtat tggctccctt ttaatcactt tgcgtcggga
     4861 agttatgctg gaaaatttga atctctctct attctctctt attaacgcga cgccagactc
     4921 ggctccgtgg atgatctcgt tggcgatttt tattgctaaa gatttgatta ccgtggtgcc
     4981 gctgctggcc gcggtgcttt ggttgtgggg gcttacagca caacggcaac tggtgataaa
     5041 aatcgctatc gcgctggcgg tcagcctgtt tgtgtcctgg acgatgggac atctttttcc
     5101 gcacgaccga ccctttgtcg aaaatatcgg ctataacttc ctgcatcatg cggcggatga
     5161 ctcattccca agcgaccacg gtacggtgat tttcaccttt gcactggcat ttttatgctg
     5221 gcatcgcctg tggtccggct cacttttaat ggtgctggcc gtcgtcattg cctggtcgcg
     5281 cgtttatctt ggcgtccact ggccgctgga tatgctcggt ggattgctgg caggtatgat
     5341 tggctgcctt agtgcccaga ttatctggca agcgatgggg cataaactct atcaacgtct
     5401 gcaatcgtgg tatcgcttct gttttgcatt accgatccgc aaaggctggg tgcgtgactg
     5461 atctctgcta aaaagtgtag tattgagcgg ctcgcttcaa taactattca gagggattat
     5521 ggaaacacgt cgcgaagagc gtatcgggca gctgctgcaa gaattaaaac gcagcgataa
     5581 gttacatctt aaagacgccg ccgccctgct tggggtttcg gagatgacga ttcgtcgcga
     5641 tctgaacaac cacagtgcgc ccgtcgtttt gctcggcggc tatattgttc tggaaccgca
     5701 tagtgccagc cattacctgt taagcgatca aaaatcccgc ctggtggaag aaaaacgccg
     5761 ggcggcaaaa ctggctgcga cactggtaga acccgatcag accctctttt ttgactgtgg
     5821 caccaccacg ccgtggatta ttgaagcgat tgataatgaa atccctttta ccgccgtttg
     5881 ttattcgcta aatacctttc tggcgctgaa agagaaaccc cattgccgcg catttctgtg
     5941 cggtggtgaa tttcacgcca gcaacgccat tttcaaaccc atcgattttc agcaaacgtt
     6001 gaataatttt tgcccggata tcgcttttta ttccgcggcg ggcgtgcatg tcagtaaagg
     6061 cgctacctgt tttaatcttg aagagttgcc ggtaaaacac tgggccatgt cgatggcgca
     6121 aaagcatgtg ctggttgtcg accacagtaa atttggcaag gtccgtccgg cgcgcatggg
     6181 tgacctgaaa cgctttgata ttgtggtgag tgattgttgc ccggaagatg agtatgtgaa
     6241 gtacgcgcag acgcaccgta ttaagttgat gtattaatga cgtataaccg gatgacgttt
     6301 cgcgccatcc ggttatcaga agattaagag aaccagctgc cgaaccactg atggaatttc
     6361 atcatcacga aatcccacat cctaccaaag aatccgccct cttccacatt ttccatcacg
     6421 atcagcggac gctgctcaat ggatttaccg ttaagctgga aatcaatggt cccgacaacc
     6481 tgaccttttt tcagcggtgc ggtaagctgc ggttccgtta acgtgtagct cgccttcagg
     6541 tttttcagct gcccacgcgg tatggtcact gagcccgctt cgcctgcccc gagattcact
     6601 tcgctcttat caccaaacca gacgcgctga gtcacaaagg tggcatcagg tttaattggc
     6661 gtcacggttt caaagaagcg gaaaccccag gtcaataatt tctcagactc atcggtcagt
     6721 tttcgccccc aacactacgg agattaaacg catatcgccc tgggtagccg aagcaaccag
     6781 attatatccc gcgcctgccg tggttcctgt cttcatgcca tcaacattca gattgctgct
     6841 ccacagcaga cggttacggt taggctgacg aattttgttg aaggtgaact ctttctcttt
     6901 atgaatggcg tactcttccg gcacatcgtg gatcaatgct ttacccagca atgccatatc
     6961 gcgcgcggta ctgaactgcc ccggcgcatc caggccgtgc accgtctgga aggtagtgtt
     7021 ggtcagaccc agttttttgg cataaccatt catcagacca ataaatgact cctggctccc
     7081 ggcaacgtaa tcggccagcg caatacaggc gtcattaccg gactggataa tcacatcttt
     7141 gttcaagtct gccaccgaaa cctgatcgcc cggtttgagg aacattaccg atgaaccacg
     7201 cagtgccgga tttcccgtcg cccaggcatc tttaccgacc gtcaccatat cggtgagttt
     7261 aatcttatcg gccttaagcg cctgcccaac cacatagctg gtcatgattt tagtcaggct
     7321 cgcgggatcc agtttctcat ccgcattgcc ttctgccagc actttaccgc tggcgtaatc
     7381 cattaaaatc catgcacgcg catccacgct cggcgcttca acggtttgtt cggccgcgaa
     7441 tgccgttggg gcaaaaagga ataaaaatgc agaacccgct gcaagaccac gaaggagaga
     7501 ggagtattgc gtcataaatg ccacccgagt atccattcaa aaaaaatacg ttatatcgcc
     7561 gtatgataag ttcggtgatt aataacgcac acacaaactt aaagaaaccg taagttggta
     7621 aagtttttaa agtttatgca atatcctgcc gcttcgctgc ttctggtgtc atttttttgc
     7681 cagctgttgc ttaaaagtta actttacttc accatcggag tcctgttcag cctgtggctg
     7741 cctgtcggaa aaggggtgag tatgattacg ctgtggggtc ggaataattc aaccaacgta
     7801 aaaaagtatt gctgacgctc gaagaactgg aactacctta tgagcaaatt ctcgcgggcc
     7861 gtgagtttgg gataaatcac gatgctgatt ttctggcgat gaaccctaac gggctggtgc
     7921 cgttgttgcg tgacgacgaa agtgatctca ttctttggga atcaaacgcc attgtccgct
     7981 atctggcggc acagtacggg caaaaacgcc tgtggatcga ctcaccggca cgtcgtgcgg
     8041 aagcggaaaa atggatggac tgggcaaacc agacgctcag taatgcccat cgagggatcc
     8101 tgatgggatt agtcagaaca ccaccggaag agcgcgatca ggccgccatt gatgccagtt
     8161 gcaaagagtg cgacgccctg tttgccctgc tcgatgcgga actggcaaaa gtaaaatggt
     8221 tctctggcga cgagtttggt gtgggcgata tcgctatcgc accgttcatc tacaatttgt
     8281 ttaacgttgg cctgacctgg acaccgcgtc caaatctgca acgctggtat cagcaactca
     8341 ctgaacgccc cgcagtgcgc aaagtggtga tgattcccgt tagctaattg cgtgggctaa
     8401 ctttaagtaa ttccccactg gactcgtcgg tgagaacgta taaataaccg tcgggtccag
     8461 tgcgaacatc acgaattcgc tgccctctgt ccgttaaaat acggccatct tctgtcactt
     8521 tgtcgccgtt gacgctcatc acaatgacat ctttatcttt cagcgcgcca ataaataatt
     8581 tttgctgcca ctgggggaat ttatcgctgt tatagaaggc catgccgctc acagcgggcg
     8641 aatctttcca gtaaaaaaca ggttgctcgg tcccggcgac gatctcccct ttcgcttccg
     8701 gtatcttaaa gcctgaatag ttgattcccc aggttgccag cggccagccg tagtttttgc
     8761 ctttttgcgg gatattaatt tcatcaccac cgcgcgggcc atgttcattc agccacagtg
     8821 cattactcca cggattcatc gccattcctt gcggattacg aatgccataa gaccagatct
     8881 cggcgcgcgc accggattcc tttataaaag gattatcatc cgggatttcg ccctgatcgg
     8941 tcagacgcac cagtttgccc tgtaatttat ccagatcctg cgccgtcggg cgctgattgt
     9001 tttcgcccag agcaataaaa agataacctt taccgtcgaa taccagccgc ccgccaaaat
     9061 ggttgccggt agacagtttt ggcatctggc ggaagacggt gcggaagtcg gtcacttttg
     9121 agagatcatc acttaagcgg ccataaccca cagcagttcc ggctttgcca tcatcgccaa
     9181 cttcggaata acttaaccag atgcggcgag actgagcaaa atcaggcgct aaaaccacgt
     9241 ccagcaggcc gccctgcccg tgcgcccaaa cgtccggaac tccggaaagc ggcgcagata
     9301 atccttttcc tgcttgccag tgacgcaact cgccgccgcg cagagtgatt aacataccgt
     9361 gattatcggg taaaaaggcc agtgcccagg gatggtcgag tttgtcttgc agtacttcga
     9421 catttaccgt tgcaggagcc gcccagagag cggaagaaag acaaataagg ggcacaagga
     9481 aaaaggattg tcgatgca
//
LOCUS       DAZPEB010000156         9317 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZPEB010000156 DAZPEB010000000
VERSION     DAZPEB010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9317
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.159"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..236
                     /locus_tag="ACHURJ_003238"
     CDS             <1..236
                     /locus_tag="ACHURJ_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HFR1282067.1"
                     /translation="TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLV
                     YEKTYTAELSSIAGTPIEAGPFDTVVLFKINYN"
     gene            388..1434
                     /locus_tag="ACHURJ_003239"
     CDS             388..1434
                     /locus_tag="ACHURJ_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFR1282068.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTCM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPDGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(1431..2222)
                     /gene="nei"
                     /locus_tag="ACHURJ_003240"
     CDS             complement(1431..2222)
                     /gene="nei"
                     /locus_tag="ACHURJ_003240"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFR1282069.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVGTRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(2258..2992)
                     /gene="pxpA"
                     /locus_tag="ACHURJ_003241"
     CDS             complement(2258..2992)
                     /gene="pxpA"
                     /locus_tag="ACHURJ_003241"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFR1282070.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            complement(2982..3914)
                     /gene="pxpC"
                     /locus_tag="ACHURJ_003242"
     CDS             complement(2982..3914)
                     /gene="pxpC"
                     /locus_tag="ACHURJ_003242"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFR1282071.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLNVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSPQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(3908..4564)
                     /gene="pxpB"
                     /locus_tag="ACHURJ_003243"
     CDS             complement(3908..4564)
                     /gene="pxpB"
                     /locus_tag="ACHURJ_003243"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFR1282072.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIELLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(4587..5330)
                     /gene="ybgI"
                     /locus_tag="ACHURJ_003244"
     CDS             complement(4587..5330)
                     /gene="ybgI"
                     /locus_tag="ACHURJ_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFR1282073.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            5601..7082
                     /gene="dtpD"
                     /locus_tag="ACHURJ_003245"
     CDS             5601..7082
                     /gene="dtpD"
                     /locus_tag="ACHURJ_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFR1282074.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGSFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGTIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(7124..8542)
                     /gene="phrB"
                     /locus_tag="ACHURJ_003246"
     CDS             complement(7124..8542)
                     /gene="phrB"
                     /locus_tag="ACHURJ_003246"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFR1282075.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACSNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHCPFIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVMLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(8539..9048)
                     /locus_tag="ACHURJ_003247"
     CDS             complement(8539..9048)
                     /locus_tag="ACHURJ_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFR1282076.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            9087..>9317
                     /locus_tag="ACHURJ_003248"
     CDS             9087..>9317
                     /locus_tag="ACHURJ_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282077.1"
                     /translation="MVNEFYGAEFLRGSILLVKILSTSSERELFYYFPSCIHFLYFLF
                     LENNSSQSLITSLLIVFQTNNRTPFSLFKKHKH"
BASE COUNT         2107 a         2573 c         2315 g         2322 t
ORIGIN      
        1 aaacgttggt tgaagaagat aaagcattgt tgattggtaa tggtctgaag ctacgtttgc
       61 tggatgaaaa cgcctcgcct tataccttca ataaatatgc tgaatacgcc gatttcacca
      121 gcgacatgtt ggtctacgaa aaaacctata cagctgaact ttcgtccatt gcaggtaccc
      181 ccatcgaggc tgggcccttc gatacagtgg tgctttttaa aattaactat aactgatttg
      241 ccatgatgcc ctttgcagcc ggaaagggca tctctttcag aaaattgctg attttcctct
      301 tttccttgat tactgttatt ttttcctggc ccggctttct ttatgctgta tcgatacacc
      361 aaagagaaaa taatgaggga gcgaaggatg ccagttttgc agtggggaat gttatgtgtg
      421 ctatcacttc tcctttctat tggtttcctc gcggtacatc tcccggcggc gctattactt
      481 gggccgatga tcgccgggat catcttcagt atgcgcggaa ttactctgca acttccccgc
      541 tccgcttttc tcgccgcgca ggccattctt ggctgcatga ttgcgcaaaa cctcactggt
      601 tcgattctca ccacgctggc cgccaactgg ccgatcgtgc ttgctatttt actggtgacg
      661 ctgctttcca gcgccatcgt gggttggtta ttggtgcgct atagctcact gcctggaaat
      721 accggtgcct ggggttcttc tccaggcggc gcagcggcaa tggtcgctat ggcgcaagat
      781 tacggcgcag atattcgcct ggtggcgttt atgcaatatc tgcgagtgct gttcgtcgcg
      841 ggggccgctg ttctggtcac ctgcatgatg ctgggtgata acgctgaagc ggttaaccag
      901 caaatcgtct ggttcccgcc agtgagcatt aatctcctgc ttacgatttt gctcgcagtc
      961 gttgccggta cggcgggatg catgctgcgt cttccttccg gcacgatgct catcccgatg
     1021 ctggcgggcg cagtgctcca gtctggtcag ctcatcacca tcgaactacc ggaatggctg
     1081 ctggcgatgg cgtatatggc aattggctgg cggattggtc ttggtttcga taaacaaatc
     1141 ttactgcggg cactacgccc actaccgcaa atcctgctgt cgatttttgc tctgctggct
     1201 atttgtgcag gtatggcgtg gggactgacc cggtttatgc atattgattt tatgactgcc
     1261 tacctcgcca ccagtcccga cgggcttgat accgtggcgg tcatcgccgc agggagcaat
     1321 gccgatatgg cgcttattat ggcgatgcaa accctgcgcc tgttcagtat tttgctgacg
     1381 gggccagcca ttgcgcggtt tatttcaacc tatgcgccga agcggtcggc ctagtgctgg
     1441 cagcctggac accagtaaaa cgggcgcgag gacaacgtgg ttttctcaat gatgccgcca
     1501 cagcgttcgc acagttcgcc atcacgatga aaaaccttaa agcgaaacag cgccccatga
     1561 tgcttattct catccacctg cccccgcgta gcgtaagaaa accgaggaat ctccagtaac
     1621 gcgtgtgcga gtgcatccag ttgcgccgca ttgagatctt tcgctttatg atttccagtc
     1681 aacccaacct gccagaggat ctccacccgc aaataattgc caagcccagc cagaaacgcc
     1741 tgatcgagca gtaatcctgc aaactgacgg ttacgaaagc gcggtgacaa taaccgttcc
     1801 tttaccacct ccggcgtcag attcggatcc agcacatcgg gaccaacgcg ttgtaaaaac
     1861 ggatgcgtgg tcagttgttc cggcctcaac atctcaatat ccgaggcgct ataaagcaga
     1921 atggttttgt cagccgtttg cagttttacc cgcaatactc gcgtggtctg cggctcttcg
     1981 ccggtatcaa ccacgcgcca gacgccgtaa agctgattat ggctgtagag cgttaagtcg
     2041 ttggaaaaat gagttaacaa cgccttacca cgcgttccca catgggtaac gtgttgaccg
     2101 ataagttgtg attgataagt ttttaactgc gggaaggcaa accagacatc agttagtggt
     2161 ttgcctttga tcgccgcctc caggttatcc gctgcacggc ggatctccgg gccttcaggc
     2221 atggtgatat ccttttttca ttctttatta atcggggtta tgctgcgaca acaatctcct
     2281 tttcggcaaa tgtagagcgt agtcggcggg cgaacgccag tgcgtgctcg ccgtcgccat
     2341 gcaggcagac tgtttgcgcc gtgaccgttg cccattcgcc ggtgatactt ttgactctgc
     2401 cgtgttgcac catctctagc gtttgcgcca gcgcctgttc ttcgttttca atcaacgcgc
     2461 ctggctgact tcgcggcacc agcgagccgt cagcctgata accgcgatcg gcaaacactt
     2521 cctcgcgcgt tgtcaggcca tattgcttgc ctgcacgaat cagctcgctt cctgccagcc
     2581 caacgagaac caatgctgga tcgcaagcgt ataccgctct ggcgatggcg tctgccagtt
     2641 gtgcttcttt cgccgcctgg ttgtacaaca tgccgtgcgg tttgacatga cgcattacgc
     2701 cgccttgcgc acgggcaatc gttgccagcg cgccaatttg atacagcgtc tgggcgtaaa
     2761 cggtttctgg cggcagctgc atggcgctgc gaccaaaatt ttccctgtcg ggaaaactcg
     2821 ggtgagcgcc aatcgcgaca ccatttttta ttgcttcacg cacgcaagcc tgcatggttt
     2881 gggcatcgcc tgcatgaaat ccacaagcaa tattggcaga ggaaaccagc gttaatagct
     2941 ctgcgtcgct ggcgcagcct tcgcccagat cggcgttcag gtcaattttc attgtgtagc
     3001 cgccacgcta attgttcgaa ataacgttgt tgatcttgcc gcgctttcag tgcctcttcc
     3061 agtgaacact ggacaaaatg aatcggctga ccgagcggaa tttgcgccag atggtacata
     3121 tcagcctcaa tgatacaggc aatacgcggg taaccaccgg tggtctgtgc gtcgttcatc
     3181 aacacaatcg gctgcccgtt atgcggcacc tgcaccacac ccggtaacaa accgtgagat
     3241 aacagttcgc gatcggtggt gcgttttaaa atttgcccct gtaagcgata gcccatgcgg
     3301 ttactttgcg ggctaagctg ccagggcgaa cgccagaatg catcctgcga ggcgcgatcg
     3361 aactcatgat attccggccc cggcaaggcg cgaatgcggt tgccccacag cagctgttta
     3421 acgccctgcg cttccataaa atcacgcttc gatttgccaa tcgggagtcg gtcaccatcc
     3481 ttcagtaaac ggccttccag cccgccaatc cccacgttga gatcggtgct gcatgacccc
     3541 attaccggcg gaacatcaat accacccgcg accgccagat aactgcgcat gccgtgctgc
     3601 gggcgtttaa gcgttaaacg ctggcctgct ttcatcggca agcgccagcc ggtccagacg
     3661 gcgttatcat ccagccgcgc ttcgcaaccg gcacccgtca gagcaaacca cccatcagtt
     3721 tcgaactcaa ccgttaactg accgagcgtg atctccagcg cgggggcatt ggcgtcatta
     3781 cccaccagca ggttagcaat gcgtaacgca ggcatatcca gtgcgccgca gtggctgata
     3841 cccgactggc gaaaaccgtg acgaccgcca tcctgcacag tggtatacat gcccgcacga
     3901 ataatcttca gcatactccc tccttctgcg gtacaaagcg cacgctgtct cccggacgta
     3961 ataagatggg ttcgtcacgc gccggatcaa acaggctgag tgaggtatga ccaatcaact
     4021 gccagccacc cggcgttgcc agcggataaa caccagtctg cggcccgccg atcccgacag
     4081 aacctgccgg aacgagcaag cgcggttcag cgcgccgtgg cgtgtgtagt tgttccggca
     4141 aactcccgag atacgggaag cccggttgaa aacctaaaaa ccagaccacg tattccacgg
     4201 aggagtgcaa ttcaacaacc tgtttttcgc tcaacccgca atgcgccgcg accaccgcca
     4261 aatccggtcc gcctgcacca ccgtaaacca ccggaatttc aataaagcga gactcaggtt
     4321 ccagggcctc gctctcctcc caccagcgtt gcaaaagctc tatggcatcc agcgccagcg
     4381 actcaggatt acgcaaaatc accgtgatat tgttcatgcc gggaatggct tcaaccacat
     4441 tcggcatatc caccagacgc tgcgccagtc gccagatccg tttctggcta gccagcgtca
     4501 ccggcggttc cagttccagc actaccgccg tttcacctat cagataacaa cgcgctcgtt
     4561 gcactttcga tccctctgat tattcgttat gcaggattag gaatatcaat aaaggtcaca
     4621 tcaagatcgg tattttcatt cagccactcg ctcaatgcgc gaataccacc acgttcagtg
     4681 gcatggtgac ctgcagcata aaaatgcaat ccctgctcgc gggctgaatg aatggtctgt
     4741 tcagaaactt cgccagtaat aaaagcatcc acgccaaaac gcgcggcgct atcgataaaa
     4801 ctttgcccgc cgcccgtgca ccaggcgacg cgctggacca cctcaggtcc ggtatcgcca
     4861 caccataacg gcttacgtcc cagacgcgct tcaatccagg aggctagttc cagtcccggc
     4921 acaggcatgg tcagctctcc ccacggcacc aacggctcaa tttcgcccat gaccgtgatc
     4981 cccagtaacg ccgccagctg tgcgttattg cccagctcag gatgtgcgtc aagcggcaaa
     5041 tgccagccat acaggttgat atcattcgcc agcaacgttt ttaaacggtt acgcttcatt
     5101 ccgcgaatga ccggagactc tcctttccag aagtagccat gatgcacaat gaccgcatcc
     5161 gcgcccagac gcactgcctc atcgagcaaa gcctggctgg cggtgacacc ggtaacaatt
     5221 ttttgcaccg tctctttgcc ttccacctgc aaaccattcg gcgcgtaatc actaatcgcc
     5281 gcgctgttca gtttttcgtt aatcagttgt tccagttcgg tgtttttcat cttcgcgcct
     5341 tccgtattct gatagctaca ccgttaacat agcgttacct cgcctgttcg tcgatagcca
     5401 atcttttgac atattaagaa acaaacccga cgccaaaaga cgcaaattgt ttatatcatc
     5461 acgcttcagc taatgtatat ttatgcaatc agacagcata ttgcataaat aatcagatct
     5521 gtatcaaagt tttaacttta aggaaacaca agcacgccgc gcaactatta cgtgacgttg
     5581 ccgtcaattg tggaattatt atgaataaac acgcatcaca gccgcgcgct atttactatg
     5641 tcgttgcgct gcaaatctgg gaatatttta gcttttacgg catgcgtgcc ctgctgattc
     5701 tctatctcac caatcaacta aaatacaacg atactcacgc ctacgagtta tttagcgcct
     5761 actgttcgct ggtgtatgtc acgccaatcc tcggtggctt tttggcggat aaagttctcg
     5821 gcaatcgcat ggcggtgatg ctgggggcgt tattgatggc gatcggtcat gtggtgttgg
     5881 gtgccagtga gatccatccg tcattcctct atctgtccct ggcgataatt gtttgtggct
     5941 atggtctgtt taaatccaat gtcagttgcc tgctcggcga gctgtatgag ccaaccgatc
     6001 cgcgtcgtga tggcagtttc tcgctgatgt atgcagcggg taacgtgggg tctattatcg
     6061 cacctattgc ctgtggctac gcccaggaag agtacagctg ggcgatgggc tttggcctgg
     6121 cggcggtggg tatgatcgcg ggtctggtca ttttcttatg tggcaatcgt catttcacac
     6181 atacccgcgg cgttaataaa aaagtactgc gtgcgacaaa ctttctcctg cctaactggg
     6241 gatggctgct ggttctgctg gtggcaacgc ctgcgctgat taccgtattg ttctggaaag
     6301 agtggtcggt atacgcctta attgtcgcga ctattattgg cctgggtgta ctggcaaaaa
     6361 tttatcgcaa agcagaaaac cagaaacagc gtaaggagct ggggctgatt gtgacgctca
     6421 ccttcttcag tatgttgttc tgggccttcg cacaacaggg cggcagctcg attagccttt
     6481 atatcgaccg cttcgttaac cgcgatatgt ttggttatac cgttccgact gctatgttcc
     6541 agtcgattaa tgccttcgca gttatgctgt gcggtgtgtt cctggcgtgg gtggttaaag
     6601 aaagcgtcgc aggtaatcgt accgtgcgca tctggggaaa atttgctctt ggtctcggcc
     6661 tgatgagcgc cgggttctgc attctgacct taagcgcccg ctggtccgca atgtatggtc
     6721 actcttctct gccactgatg gtattaggcc tggcggtgat gggctttgcg gaactgttta
     6781 tcgacccggt tgccatgtcg caaattacgc gcattgaaat ccccggtgtg accggcgtat
     6841 taaccggcat ttatatgctg ctttccggca cgattgcgaa ctatctcgcg ggcgtgattg
     6901 ccgatcagac atcgcaggct tcgtttgatg cttccggggc gatcaactac tccatcaatg
     6961 catatattga agtatttgat caaattacct ggggcgcact ggcgtgtgta ggactggtac
     7021 tgatgatttg gctgtatcag gcgctgaaat tcagaaaccg cgcgctggcg ctggagtcat
     7081 aataggccag caccatacct gataaaacgc tcgctgcgtt ttatcaggct cctttcctcg
     7141 cttcttcgta cgctgccagc gttcgcagcc ttgcctcttt gtgatcaact atcggttgcg
     7201 gataatccag catcacacct gctttctgcg cccacttcca cggctcatgc accgctttcc
     7261 ctggtacatc gcgcagctcc ggtaaccatc gacgaataaa ctcgccctca cggtcaaatt
     7321 tctcgccctg ggttgtcggg ttgaaaatac gaaaatacgg cgctgcatcg gttccggttg
     7381 aagcggccca ctgccagcca ccgttattgg ctgccaaatc accatcaatc agctgcgaca
     7441 tgaaatatcg ctcgccttcg cgccagtcga tcaacaaatc tttcaccaga aaactggctg
     7501 taatcatccg tagccggtta tgcatccagc cagtgctgtt aagctgacgc atggcagcat
     7561 cgacaatcgg gtatcccgtt ttgccttcct gccaggcctg taaatgtgcg ggattgctct
     7621 gccactgcac acgatccgtc caggcaataa acggacaatg tttacacaac gaggggtaat
     7681 acgtcatcag atgacggtag aactcgcgcc agatcagctc actaagccag acactaccgg
     7741 ccccaccgtc cagcgcctgc ggctgttccg ccaacaagcg atgcaaacac tggcgaggcg
     7801 ataacccgcc cgttgccagg ctggcggaca aacgactggt gccttccact gccggaaaat
     7861 ctcgttgttg ctcatattct ccggcaccgt tctggcaaaa ctggcgtaat tgcgcaatcg
     7921 ccgctttttc ttccaccggg aaatgcgcag tatcgaaaga ctgacgagga taattcagcg
     7981 taatggatgg cgagggctct atcgatccgc tactacgaac ttttggcgca gcgacgcact
     8041 ccggcatccc ttcccgcagc cgtttcagcc aggcattctt aaaaggcgta aagactttgt
     8101 acatctcgtg attaccggtc atcaccgcgc caggcggcag gatcacgctg tcatcaaatc
     8161 cttcacacac cacgttacgc agcgctcttt caacttccac atcccgcgcc cgctcattca
     8221 cttcatactg atagttataa aacaggtggg taacgctgtt ttccgcgcac acctgtttaa
     8281 ctatttcgac actggcgaca aagtcatcca cttcacggaa caataaagga ataccttttt
     8341 ccgcaagcgc tatttgtagc ccattcagtt gagcattgat aagttcagcc tgacgcggcg
     8401 acatgttatg cgtcgcccac tggcgtggtg tagcgatata caacgccagc acgcgtgcag
     8461 acgaattgct gcaggcggca gccagtgcga gattatcgtg caaacgtaaa tcctggcgaa
     8521 accagaccag atgggtagtc ataaaactcc tgaatggcgc aagttaatca cacgcggcca
     8581 aagttcgaaa taacgctgcc ctgaaagcca ggcgtcagga taaagcgcca tatagtgttt
     8641 gatacgcatc agcggcgcaa gaagtggttg ctcgccacgg cgataactgt cgataagcgc
     8701 agccagctgc tggcgttctg tggaatcaat atgcgggcga aaataaccct gaacgtgcat
     8761 caaaacattg gtgtgatcgc gcgggttggc ggggtgagaa agcaaaacaa ctacgcgttg
     8821 gcggtactgg ttgtaatagt catccagatt ttgccactgg tgaatatcag ccacgaacgg
     8881 gccaagtttg cggtactccg gctgagaatg cgccagaaag acgagtttat agcggctgtg
     8941 ataatcgaga agcgccccgc gcgttaagcc atgttctttc agtcgatgta actcatgaag
     9001 ggcaaagata cggattaggg tgctgtcatc aaatcgttgt agattcatta ctgctcctga
     9061 acggtataaa ggagtaagtg tagacgatgg taaatgaatt ttatggggca gagtttttgc
     9121 ggggaagtat tttgttggta aaaatattat caacttcatc tgagcgagaa ttattctact
     9181 atttcccgag ctgcattcat tttttgtatt ttttattttt agaaaataac agttctcaaa
     9241 gcttaattac ctcactatta attgtatttc aaactaacaa tcgcactcca ttttctctct
     9301 ttaaaaaaca taaacat
//
LOCUS       DAZPEB010000157         9076 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZPEB010000157 DAZPEB010000000
VERSION     DAZPEB010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9076
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.160"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(21..464)
                     /locus_tag="ACHURJ_003249"
     CDS             complement(21..464)
                     /locus_tag="ACHURJ_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFR1282078.1"
                     /translation="MAELQKVDDWLSALLANLEPAARSRMMRQLAQELRRTQQQNIRM
                     QRNPDGSSYEPRRVTARSKKGCIKRQMFTKLRTTKYLKTAASADSASVQFEGRVQRIA
                     RIHHYGLSDRVSRKGIYTIYAKRQLLGFNVQSIELIERMFIKNLT"
     gene            complement(457..888)
                     /locus_tag="ACHURJ_003250"
     CDS             complement(457..888)
                     /locus_tag="ACHURJ_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1282079.1"
                     /translation="MNKPQSLRHALNKAVPYVRNNPDKLHLFVDNGSLVASGASSMSW
                     EYRYTLNVVIEDFSGDQNLLMAPVLLWLRDNQPDAINNPALREKLFTFEVDILRNDVC
                     DISLNLQLTERVLVSTDGSVSNVEAVAEPDEPEEMWTVKRG"
     gene            complement(851..1099)
                     /gene="lysC"
                     /locus_tag="ACHURJ_003251"
     CDS             complement(851..1099)
                     /gene="lysC"
                     /locus_tag="ACHURJ_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461641.1"
                     /note="LysC is an Rz1-like component of a phage lytic
                     system, substantially overlapping although not fully
                     embedded in the gene for the Rz-like LysB component;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1-like lysis system protein LysC"
                     /protein_id="HFR1282080.1"
                     /translation="MLTLSGCGNVRHSPEVQLTVSGCPRVTPCRLEQAAPRTNGDLNA
                     LLDETEAAWAVCADKVDMIIACQERNSEQTTIPAPRPQ"
     gene            complement(984..1412)
                     /gene="lysB"
                     /locus_tag="ACHURJ_003252"
     CDS             complement(984..1412)
                     /gene="lysB"
                     /locus_tag="ACHURJ_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341071.1"
                     /note="The gene for this Rz-like phage lysis system
                     protein may overlap extensively with the gene for the
                     other spanin subunit, the Rz1-like protein in the outer
                     membrane; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz-like lysis system protein LysB"
                     /protein_id="HFR1282081.1"
                     /translation="MNRLLLVVLALLLAALGWQTWRLADASQTISTQADELQSKSQAL
                     AKSNSQLISLSILTETNNREQARLYAEAEQTSVLLRQRQRRIEELKRENEDLRRWADT
                     PLPADIIRLRERPALTGGAAYRQWLSASDAVSAGAGSAAH"
     gene            complement(1409..1786)
                     /locus_tag="ACHURJ_003253"
     CDS             complement(1409..1786)
                     /locus_tag="ACHURJ_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNZ54_00345 family protein"
                     /protein_id="HFR1282082.1"
                     /translation="MKKKLISGLFLMLWMALLIAAMVYPQGIFPVLAASGVWVACLLT
                     WAVIPVALASLIKNGPLWQELRASLLKTITRKENVFISWVIRLLIVVSLAWTGWAITL
                     VFYLLTVIAFWITHNQMAQQVAA"
     gene            complement(1788..2300)
                     /locus_tag="ACHURJ_003254"
     CDS             complement(1788..2300)
                     /locus_tag="ACHURJ_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFR1282083.1"
                     /translation="MNPSIVKRCLVGAVLAIAATLPGFQQLHTSVEGLKLIADYEGCR
                     LQPYQCSAGVWTDGIGNTSGVIPGKTITERQAAEGLISNVLRVERALEKCVKQQPPQK
                     VYDAAVSFAFNVGTGNACSSTLVKLLNQRRWADACRQLPRWVYVKGVFNQGLDNRRAR
                     EMAWCLQGAN"
     gene            complement(2281..2496)
                     /locus_tag="ACHURJ_003255"
     CDS             complement(2281..2496)
                     /locus_tag="ACHURJ_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HP1 family phage holin"
                     /protein_id="HFR1282084.1"
                     /translation="MTLERISAFITYCIAVVLAWLGDLSIKDASTLGGLMIGVLMLAI
                     NWYYKHKAYQLLRDGQISREDYESINR"
     gene            complement(2500..2703)
                     /locus_tag="ACHURJ_003256"
     CDS             complement(2500..2703)
                     /locus_tag="ACHURJ_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFR1282085.1"
                     /translation="MKVRAHQYDTVDALCWRHYGRTQGVTEQVLKANPGLAEYGPFLP
                     HGLQVELPDIPTTTTVQTVQLWD"
     gene            complement(2703..3167)
                     /locus_tag="ACHURJ_003257"
     CDS             complement(2703..3167)
                     /locus_tag="ACHURJ_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFR1282086.1"
                     /translation="MKFVAPEQAPEQAEIIRNTPFWPDVDLSEFRSVMRTDGTVTQPR
                     LKQVALSEISEVNAELYEFRRRQQMLGYASLAEVPAEQLDGKSERIQHYFNAVYCWAR
                     AMLNERFQDYDATASGAKRGEELAEASGDLWRDARWAISRVQDAPHCTVELI"
     gene            complement(3263..3913)
                     /locus_tag="ACHURJ_003258"
     CDS             complement(3263..3913)
                     /locus_tag="ACHURJ_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HFR1282087.1"
                     /translation="MSLSPARQHRLRVQAEQAVREGGSVRHASGYDLMLLQLAEDRRR
                     LKGVQSTVKKAEIKVELLPKYAAWAEGVLAAGGAQQDDVLMYVMLWRIDAGDYAGALE
                     IGRHALRHGWVMPLGNRNVQTVLTEEMADAAQSAMLAATGFDADLLLQTLELTDGLDM
                     PDQSRARLHKAIGAVLSESNPASALNHLNHALQLDPRCGVKKDKQQLERRLRNDSR"
     gene            complement(3917..4975)
                     /locus_tag="ACHURJ_003259"
     CDS             complement(3917..4975)
                     /locus_tag="ACHURJ_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HFR1282088.1"
                     /translation="MKKNTRFAFNAYLQQLARLNGVAVEELSSKFTVEPSVQQTLEDQ
                     IQQSAAFLTLINVTPVTEQSGQLLGLGVGSTIAGTTDTTAKEREPVDPTLMVDVEYKC
                     EQTNFDTVLTYAKLDLWAKFQDFQVRIRDAIVKRQALDRIMIGFNGVKRAKTSNRSEN
                     PLLQDVNKGWLQKIREDAPDHVMGSTTTGGETTPGAVKVGKGGEYANLDAVVMDAVNE
                     LIDVVYQDDDDLVVICGRELLSDKYFPLVNKEQENSEKLAADMIISQKRMGGLQAVRA
                     PFFPPNALLITRLDNLSIYWQEDTRRRSVIDNPKRDRIENFESVNEAYVVEDYRCAAL
                     VENIQISDFSAAAAETGA"
     gene            complement(4992..5825)
                     /locus_tag="ACHURJ_003260"
     CDS             complement(4992..5825)
                     /locus_tag="ACHURJ_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFR1282089.1"
                     /translation="MTVKAKRFRIGVEGATTDGREIQREWLEQMAASYNPAVYTALIN
                     LEHIKSYLPDSTFNRYGKVTALFAEEITEGPLAGKMALYADVEPTESLVELVKKGQKL
                     FTFMEVSPKFADTGKAYLVGLAATDDPASLGTEMLTFSASAAHNPLANRKQNPANLFT
                     AAEETVIELEEVQDDKPSLFARVTALFTKKEQSDDARFSDVHKAVELVATEQQNLSAR
                     TEKSLSEQEERLSELETALQAQQTAFNELVDKLSHEDSRQDYRQRATGGNAPADTLTN
                     C"
     gene            5968..7734
                     /locus_tag="ACHURJ_003261"
     CDS             5968..7734
                     /locus_tag="ACHURJ_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HFR1282090.1"
                     /translation="MNTTLTPADLDPRRQAMLLYFQGYRVARIAEMLGEKVATVHSWK
                     KRDKWGDYGPLDQMQLTTAARYCQLIMKEHKEGKDFKEIDLLARQSERHARIGKFNNG
                     GNEADLNPNVANRNKGPRRQPEKNVFTDDQIEKLEKIFHSSMFNYQRHWWEAGKTNRI
                     RNLLKSRQIGATFYFAREALIDALLTGRNQIFLSASKAQAHVFKQYIIDFAKEVEVEL
                     KGDPMVLPNGATLYFLGTNARTAQSYHGNLYLDEYFWIPKFQELRKVASGMAIHKKWR
                     QTYFSTPSSLTHSAYPFWSGALFNRGRNKADKVDIDLSHSNLAPGRLCADGQYRQIVT
                     VEDAVRGACNLFDLDQLRMEYSPDEYQNLLMCEFVDDLASVFPLSELQACMVDSWEVW
                     TDFHALALRPFGWREVWIGYDPAKGTQNGDSAGCVVVAPPAVPGGKFRILERHQWRGM
                     DFRAQADAIKKLTEQYNVTYIGIDSTGVGHGVYENVKAFFPAVREFVYNPNVKNALVL
                     KAYDIISHRRLEFDAGHTDIAQSFMAIRRATTASGNRPTYEASRSEEASHADLAWATM
                     HALFNEPLQGESANTSNIVEIF"
     gene            7734..8765
                     /locus_tag="ACHURJ_003262"
                     /pseudo
     CDS             7734..8765
                     /locus_tag="ACHURJ_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(8872..>9076)
                     /locus_tag="ACHURJ_003263"
     CDS             complement(8872..>9076)
                     /locus_tag="ACHURJ_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005007304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282091.1"
                     /translation="RGLNKSLSTYSDVYELLTRELLLLDEKHKGKNNWNSVFVAGLIR
                     PDVIRWILCELHLGNRSPDGRLI"
BASE COUNT         2039 a         2637 c         2333 g         2067 t
ORIGIN      
        1 caattgaaaa caaataaaaa tcaagttagg tttttaatga acattctttc aattaactca
       61 atagattgga cattaaaacc taataactgc cttttcgcat agatggtata aatacctttt
      121 cgactgacgc gatcactcaa gccgtaatgg tgaatacggg caatgcgctg caccctacct
      181 tcaaactgta cgctggcaga atcggcgctg gctgcagttt tcaggtattt tgtggtgcgc
      241 agctttgtaa acatctgacg tttgatgcac ccctttttgc tgcgtgctgt taccctgcgc
      301 ggctcataac tgctgccatc aggattgcgc tgcatcctga tgttctgctg ctgtgtccgg
      361 cgcagttcct gcgccagctg gcgcatcatg cggcttcttg cggctggctc cagattcgcc
      421 aataatgcac tcagccagtc gtccaccttc tgcaattcag ccacgtttca ccgtccacat
      481 ttcttcaggt tcatcaggtt ccgctaccgc ttcaacgttc gacacactgc cgtcagtgct
      541 gaccagcaca cgctccgtca gttgcaggtt aaggctgata tcacagacat cgttgcgcag
      601 aatatccacc tcaaaggtga atagcttttc ccgtaacgcc gggttattga tcgcatcggg
      661 ctggttatcc cgcagccaca gcaaaaccgg ggccatcagc agattctggt cgccgctgaa
      721 atcttcaatc accacgttca gggtgtagcg gtactcccat gacatggagc tagccccgga
      781 ggcaaccagc gaaccgttat ccacaaacag atgcagtttg tccgggttat tacggacata
      841 aggcactgct ttattgaggg cgtggcgcag ggattgtggt ttgttcactg tttcgctcct
      901 gacacgcaat aatcatgtcc actttgtctg cacagaccgc ccaggcggcc tccgtttcat
      961 ccagcaacgc attcagatca ccgttagtgc gcggcgctgc ctgctccagc cgacacggcg
     1021 tcactcgcgg acaaccactg acggtaagct gcacctccgg tgagtgccgg acgttcccgc
     1081 agccggataa tgtcagcagg caaaggagta tcagcccagc gacgtaaatc ctcgttctca
     1141 cgtttcagtt cctcaatccg gcgttgtcgt tgtctcagca gtacactggt ctgttctgct
     1201 tcggcataga gccgcgcctg ctcccggtta ttggtttcag tcagaatgga caggctgata
     1261 agctggctgt tgctctttgc cagtgcctgg cttttgctct gcagctcgtc tgcctgcgtg
     1321 ctgatggtct ggctggcatc agccagccgc cacgtctgcc agcccagcgc cgccaataat
     1381 aacgccagca caaccagcag caaccggttc atgcggctac ctgttgcgcc atctgattat
     1441 gggtgatcca gaaggcaata acggtcagca gataaaagac cagggtaatg gcccaccccg
     1501 tccaggcgag actgacgaca atcagcaatc gtatcaccca gctgataaat acgttttctt
     1561 ttcgggtaat tgtcttcagt aaagatgccc tcaactcctg ccagagcggg ccattcttaa
     1621 ttaacgaagc cagtgctacc ggaattaccg cccatgtcag taaacaggct acccaaacgc
     1681 cggacgctgc cagtaccgga aaaatcccct gcggatacac cattgctgcg attaacagcg
     1741 ccatccataa catcagaaac agcccgctga ttaatttctt tttcatttca gtttgctccc
     1801 tgtaaacacc aggccatctc ccgcgcacgg cggttatcca gcccctgatt aaaaacacct
     1861 ttcacataaa cccagcgcgg caactgtcgg cacgcatccg cccagcgccg ctgattgagc
     1921 aatttcacca gcgtggaact gcaggcattg cccgtaccca cgttgaaggc aaacgacacc
     1981 gcagcgtcat acaccttctg cggcggctgt tgcttcacac acttttccag cgcccgctcc
     2041 acacgcagca cgttggagat cagcccttct gctgcctgtc gctccgtaat ggttttgccg
     2101 ggaatgacgc ccgacgtatt accaataccg tcggtccaga cacccgcgct gcactgatac
     2161 ggctgcagac gacaaccttc gtaatcggca atcagtttca gtccctccac ggaggtgtga
     2221 agctgctgaa aacccggcag cgtggcagca atagccagca cggccccgac aaggcagcgt
     2281 ttaacgattg atggattcat agtcctcccg cgagatctgc ccgtcgcgca gaagctggta
     2341 ggctttgtgt ttgtagtacc agttgatagc cagcatcagc acaccaatca tcaggccgcc
     2401 cagcgttgag gcatccttga tggacaaatc gcccagccag gccagcacga cggcgatgca
     2461 atacgtgata aaggcgctga ttcgctcaag cgtcataatt cagtcccata gctggacggt
     2521 ctgcacggtg gtggtggtcg gaatgtccgg cagctccacc tgcagcccgt gaggtaaaaa
     2581 ggggccgtat tcggcaagcc ccggatttgc cttcagtacc tgctccgtga caccctgcgt
     2641 gcgcccgtaa tgacgccagc aaagcgcgtc caccgtgtca tactgatgcg cacgcacttt
     2701 catcagataa gctccactgt gcagtgcggc gcgtcctgca cccggctgat ggcccagcgg
     2761 gcgtcacgcc acaaatcacc gcttgcttcc gccagttctt cgccccgctt cgcaccggat
     2821 gccgtggcgt catagtcctg gaaacgttcg ttgagcatgg cgcgagccca gcagtaaacc
     2881 gcgttgaaat agtgctgaat gcgctcactt ttcccgtcca gctgttccgc cggaacctca
     2941 gccagcgacg cataccccag catctgctgg cgtctgcgaa actcatacag ctctgcgtta
     3001 acctccgaaa tttccgacag tgcaacctgc tttaaacgcg gctgcgtcac cgtgccgtca
     3061 gtgcgcatga cactgcgaaa ctccgacagg tccacatcag gccagaacgg cgtatttcta
     3121 atgatttccg cctgttccgg tgcctgttct ggcgcaacaa acttcatgct gctttctcct
     3181 gaaataaagg gcggtggacg gggttttgat gtggctgtgc ctttcgccac cccgtgccgc
     3241 ccgtgcgcgg gggcacgttc tgtcagcggc tgtcattgcg cagtctgcgc tccagctgct
     3301 gtttgtcttt tttcacgcca cagcggggat cgagctgtaa cgcatggttg agatgattaa
     3361 gggcggaagc cggattgctt tcactcagga ccgcgccaat cgctttatgc agacgtgccc
     3421 gtgactggtc cggcatatcc agaccgtctg tcagctccag cgtctgcagc aacagatcgg
     3481 catcaaagcc ggtggcggca agcattgcgc tctgcgctgc atctgccatt tcctctgtca
     3541 gcacggtctg cacgttgcgg ttgcccagcg gcatcaccca tccatgacgc agggcatgac
     3601 gcccgatctc cagcgccccg gcataatctc cggcatcaat gcgccacaac atcacgtaca
     3661 tcagcacgtc atcctgttga gcgcctccgg cagccaggac accctccgcc caggcggcgt
     3721 acttcggcag cagctccact ttgatttccg cttttttgac cgtggactga acgcccttga
     3781 gacggcggcg gtcttccgcc agttgcagca gcatcaggtc atagcccgat gcgtggcgaa
     3841 cactgccacc ctcacggacg gcctgttcag cctgaacgcg caggcgatgc tgccgtgcgg
     3901 gactcaggct catggattac gctccggttt cggctgcggc ggcgctgaaa tcactaatct
     3961 ggatgttttc caccagtgcg gcgcagcggt agtcctcaac cacataggct tcgttaacgg
     4021 attcaaagtt ttcaatccgg tcacgtttcg ggttgtcgat aactgaacgg cggcgggtgt
     4081 cttcctgcca gtagatggac aggttatcca gacgggtgat cagcagcgca ttcggcggga
     4141 agaacggcgc acgcacggcc tgcaggccac ccatgcgttt ctgactgatg atcatatcgg
     4201 cagccagttt ttcactgttt tcctgctctt tgttgaccag cgggaaatac ttgtcagaca
     4261 gcagttcacg accgcaaatc accaccagat cgtcatcgtc ctggtagacc acgtcgataa
     4321 gctcattgac ggcatccatc accacggcgt ccaggttggc atattcgcca cctttcccga
     4381 ctttcactgc gcccggtgtg gtttcaccgc ccgtggtggt gctgcccatg acgtgatccg
     4441 gtgcatcctc acggattttc tgcagccagc ctttattcac atcctgcagc agcgggtttt
     4501 cgctacggtt ggaggttttc gcacgcttca cgccattaaa gccgatcatg atgcggtcca
     4561 gtgcctgacg tttcacgatg gcgtcacgga tacgcacctg aaaatcctga aacttcgccc
     4621 acaggtccag cttcgcgtag gtcagcaccg tgtcaaagtt ggtctgctcg catttatatt
     4681 ccacatcgac catcagcgtc ggatcgacag gttcacgctc tttcgcagtg gtatcagtgg
     4741 ttccggcaat ggtgctgcca actcccaacc ccagcagctg accggactgc tcagtcactg
     4801 gcgtgacgtt aatcagagtc aggaaagcgg cggactgctg gatctggtct tccagcgtct
     4861 gctgcacgga cggctctacg gtgaacttgc tggacagttc ttcaactgcc acaccgttca
     4921 gacgcgccag ttgctgcagg taagcgttaa aagcaaagcg ggtattcttc ttcatcaggt
     4981 tttgtgctcc atcagcaatt ggtcagagtg tcagcggggg cgttaccgcc tgttgcacgc
     5041 tggcggtagt cctggcggct gtcttcatga ctcagcttgt ccaccagttc gttaaaggcg
     5101 gtttgctgtg cctgcagggc agtctccagc tcagacaggc gttcttcctg ctcagacagg
     5161 gatttttcgg tgcgtgcgct caggttctgc tgctcagtgg cgaccagctc cacggcctta
     5221 tgcacatcag agaaccgggc gtcatcggac tgctcttttt tggtaaacag cgccgtgacg
     5281 cgggcaaaca gggacggttt gtcgtcctgg acttcttcca gttcgatcac cgtttcctct
     5341 gcggcggtaa agagattggc gggattctgc ttgcggtttg ccagcgggtt atgggctgca
     5401 ctggcgctga atgtcagcat ttcagtgccc agactggcag ggtcatcagt ggcagccagg
     5461 ccgactaggt aggctttgcc cgtatcagcg aacttcgggc tgacttccat aaaggtgaat
     5521 aatttctggc cttttttcac cagttccacc agggactccg ttggctcaac gtcagcatac
     5581 agcgccatct tgcctgccag cggaccttcc gtgatttctt cagcaaacag cgccgtcacc
     5641 ttgccgtagc ggttaaaggt gctgtccggc agataagact tgatgtgctc aaggttaatc
     5701 agcgcggtat acaccgccgg gttatagctg gctgccatct gttccagcca ttcacgctgg
     5761 atttcgcgtc cgtcggtggt ggcaccttcc accccgatgc gaaaacgctt tgctttcact
     5821 gtcatgagcc gtgctccgtt agaaaaaact tactggagcc ttatggttgc ggtgataggg
     5881 gcagtgaaac aatgcgcggt atttgtaccg acaaccacac aaaccgcagg cggggaaagc
     5941 cttcattcaa ggctgtaggt ttgtgccatg aacaccacac tgacacccgc agatctcgat
     6001 ccccgtcggc aggccatgct gctgtacttt cagggatacc gcgtagcccg cattgctgaa
     6061 atgctgggcg agaaagttgc aaccgttcac agctggaaaa aacgcgacaa gtggggtgac
     6121 tatgggcctc tggatcagat gcagctcacc accgccgcac gctactgcca gctcatcatg
     6181 aaggagcaca aagaagggaa agatttcaaa gagattgacc tgctggcgcg ccagtctgag
     6241 cgccacgcgc ggatcggcaa gtttaacaat ggcggcaacg aagccgactt aaaccctaac
     6301 gtcgccaacc gcaacaaagg cccacgccgt cagcctgaaa agaatgtttt caccgatgac
     6361 cagattgaga agctggaaaa aattttccat tcctccatgt tcaactacca gcgccactgg
     6421 tgggaagccg gaaaaaccaa ccgcatccgc aacctgctga agtcacgcca gatcggcgcg
     6481 accttctatt ttgcccgtga agccctgatt gacgccctgc ttaccggacg taaccagatt
     6541 ttcctttctg ccagtaaggc acaggcccac gtcttcaaac agtacatcat cgactttgcc
     6601 aaagaagtgg aggtggagct gaaaggcgat ccgatggtgc ttcccaacgg ggccacactg
     6661 tatttcctcg gcaccaatgc ccgcactgcc cagagttatc acggtaacct gtatctggat
     6721 gaatatttct ggataccgaa atttcaggag ctacgcaaag tggcttccgg tatggctatt
     6781 cacaagaaat ggcggcagac ctatttttcc acaccatcca gtctgaccca cagtgcttat
     6841 ccgttctggt ccggtgcgct gttcaaccgt gggcgcaaca aagctgacaa ggtggacatc
     6901 gacctgtccc acagcaatct ggcccccggc cggctgtgcg cagacgggca ataccgccag
     6961 atagtcaccg tggaagatgc ggtgcgcggc gcctgtaacc tgttcgacct tgaccagttg
     7021 cgcatggagt acagccctga cgaataccag aacctgctga tgtgcgagtt cgtggacgat
     7081 ctcgcgtccg tgttcccgct cagcgaattg caggcgtgca tggtggacag ctgggaagtc
     7141 tggaccgact ttcatgcact ggccctgcgc ccgtttggct ggcgcgaagt gtggatcggt
     7201 tatgacccgg caaaaggtac gcagaacggc gacagcgccg gatgcgtggt ggtggcaccg
     7261 ccagccgtgc caggcggtaa gttccgcatt cttgagcgtc accagtggcg cggaatggac
     7321 tttcgcgccc aggctgacgc catcaaaaaa ctgactgaac agtacaacgt gacctatatc
     7381 ggcatcgact caaccggcgt tggtcacggg gtttacgaga acgtgaaagc gttttttcct
     7441 gccgtccggg agtttgtcta caaccccaac gttaaaaacg ccctggtact caaggcctac
     7501 gacattatca gccaccgccg tcttgagttt gacgccgggc acaccgacat tgcgcagtca
     7561 ttcatggcaa tccgtcgcgc caccaccgcc agcggcaacc gcccgaccta tgaagccagc
     7621 cgcagcgaag aagccagcca tgccgatctg gcctgggcaa caatgcacgc actgtttaac
     7681 gaaccgctgc agggcgagtc cgccaatacc agcaatattg tggagatttt ttgatgggaa
     7741 agagtaagaa gaaccgcgct gcggcgacga aacagaccca gcataaaaac cagacttcag
     7801 ccgaagcatt cagcttcggc gatcccgttc cggttctgga ccgccgtgaa ctactggact
     7861 atgtggaatg cgtacagaca gatcgctggt atgagccgcc agtgagtttt gacggactgg
     7921 cgcgcacctt ccgcgccgcc gtgcatcaca gttcaccaat tgcggtgaaa tgcaacattc
     7981 tgatcagtac ctacatccct cacccgctgc tcagccagca ggctttttca cgttttgtgc
     8041 aggattatct ggtatttggt aacgcttacc tggagaaacg cacaaaccgg ttcggagagg
     8101 tcatcgctct tgagcctgct ctggcaaaat acacccgacg cggattagac ctggatacct
     8161 actggtttgt gcaatacggc atgaccacgc agccgtatca gttcacgaaa ggaagcatct
     8221 ttcatctgat ggaaccggac atcaaccaag agatctacgg cctgccaggt tacctttctg
     8281 ccattccgtc agccctgctc aacgagtccg ccacgctgtt ccgccgatag tattacatta
     8341 acggcagtca tgcaggcttc atcatgtaca tgaccgatgc cgcgcaaaac caggaggatg
     8401 tgaacaacct ccgcaatgcg atgaaaagcg ccaaaggacc aggtaacttc cgcaatctgt
     8461 ttatgtactc gcctaacggc aaaaaggacg ggcttcagat catcccgttg tcagaagtcg
     8521 cggcgaagga tgagtttctg aacatcaaga acgtgagccg ggatgacatg atggcggcgc
     8581 atcgtgtgcc accgcaaatg atggggatta tacctaataa tgtcgggggg tttggggatg
     8641 tggagaaggc gagcctggag tttgtacgta acgagttaat accgttacaa aaaagaatga
     8701 tggaaataaa taaattggta agtgagaaca ttgttaaatt tgatagttat aaactagcta
     8761 cttaacgtaa tcactaaatg aggttcttta acacagctaa aaaatttatt aatagccagc
     8821 agtttttatg aatttcttca gcgtgcagat acacgctgaa gatgcaaaaa atcatatgag
     8881 acgaccgtct ggacttctgt tacctaaatg taattcacag agtatccacc taatcacatc
     8941 aggtcgaatt aacccagcta caaaaaccga attccaatta tttttacctt tatgtttttc
     9001 atccaacagc aacaactcgc gagtcaacaa ctcatataca tcgctataag tagaaagact
     9061 cttattcagt cctcta
//
LOCUS       DAZPEB010000158         9045 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZPEB010000158 DAZPEB010000000
VERSION     DAZPEB010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9045
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.161"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            195..1028
                     /gene="ppsR"
                     /locus_tag="ACHURJ_003264"
     CDS             195..1028
                     /gene="ppsR"
                     /locus_tag="ACHURJ_003264"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFR1282092.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRTANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            1184..2230
                     /gene="aroH"
                     /locus_tag="ACHURJ_003265"
     CDS             1184..2230
                     /gene="aroH"
                     /locus_tag="ACHURJ_003265"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFR1282093.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            2362..2553
                     /gene="hemP"
                     /locus_tag="ACHURJ_003266"
     CDS             2362..2553
                     /gene="hemP"
                     /locus_tag="ACHURJ_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFR1282094.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(2557..3993)
                     /gene="selO"
                     /locus_tag="ACHURJ_003267"
     CDS             complement(2557..3993)
                     /gene="selO"
                     /locus_tag="ACHURJ_003267"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFR1282095.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNIELANTLSIP
                     SSLFKNGAGVWGGEALLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TAEPGAMLMRVAPSHLRFGHFEHFYYRRESEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLIQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(4056..4769)
                     /gene="ydiV"
                     /locus_tag="ACHURJ_003268"
     CDS             complement(4056..4769)
                     /gene="ydiV"
                     /locus_tag="ACHURJ_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFR1282096.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQVLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTAEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            complement(5015..5479)
                     /locus_tag="ACHURJ_003269"
     CDS             complement(5015..5479)
                     /locus_tag="ACHURJ_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFR1282097.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKQQASIGTQIDKDKLLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            complement(5557..6306)
                     /gene="btuD"
                     /locus_tag="ACHURJ_003270"
     CDS             complement(5557..6306)
                     /gene="btuD"
                     /locus_tag="ACHURJ_003270"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFR1282098.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVGLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLILTI"
     gene            complement(6306..6857)
                     /gene="btuE"
                     /locus_tag="ACHURJ_003271"
     CDS             complement(6306..6857)
                     /gene="btuE"
                     /locus_tag="ACHURJ_003271"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFR1282099.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            complement(6920..7900)
                     /gene="btuC"
                     /locus_tag="ACHURJ_003272"
     CDS             complement(6920..7900)
                     /gene="btuC"
                     /locus_tag="ACHURJ_003272"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFR1282100.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISLGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELLIGVVTATLGAPVFIWLLLKARR"
     gene            complement(8109..8546)
                     /locus_tag="ACHURJ_003273"
     CDS             complement(8109..8546)
                     /locus_tag="ACHURJ_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282101.1"
                     /translation="MAENVAHISRFRKKKENAYNVSNNQVTGSGNDGGDGMNDDLERR
                     VSCLESDVTEIKNNLITLTTRSESFATKSDVLEIREGLRLEMAESRQSLKSEMADLRQ
                     SLKVEMAEHRTELQKSFANQTWLLTGIVLSAMAVLVAVVTVIK"
     gene            complement(8546..8908)
                     /locus_tag="ACHURJ_003274"
     CDS             complement(8546..8908)
                     /locus_tag="ACHURJ_003274"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282102.1"
                     /translation="MKFHLDELKRMATLDNFARFLNESSTNEKCLSCGDTDMYMYLTN
                     IVEVGPEPKTAEECDLGTFVMLDYIGPFTGYPGYEGHDRENIHNYEFRLTCNRCGFVH
                     RYSARAFMNWVGKQGDEK"
     gene            complement(8911..>9045)
                     /locus_tag="ACHURJ_003275"
     CDS             complement(8911..>9045)
                     /locus_tag="ACHURJ_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFR1282103.1"
                     /translation="MNGGNILTLQKILGHAKIQTTMIYAHLAPDYLQDAVRFNPLGGA
                     "
BASE COUNT         2389 a         2395 c         2051 g         2210 t
ORIGIN      
        1 gttgcgcaaa cctgctctat tcgacaattt ccacataaac catcggtata aacgaaacca
       61 taaaaatgaa atgctgtttt cataaaaaat aaaattgaag gttcatttta taaaccagac
      121 attacctcct acgcttctgt gcgtttttaa tttatgcttt catagaatta tgtctgcatc
      181 acgggaagaa caaaatggat aatgctgttg atcgccacgt tttttatatt tctgatggta
      241 cggcaataac tgcggaggta ttaggacacg cagtaatgtc acaatttccc gtcactatca
      301 gcagcattac gctgccgttt gtcgaaaatg agagccgtgc acgggcagtg aaggatcaga
      361 ttgacgcaat ttatcaccag acaggtgtgc gcccgctggt cttctactcc atcgtgttgc
      421 cggagattcg cgccatcatc ttgcaaagtg aaggcttttg ccaggatatc gttcaggcgc
      481 tggttgcccc gctacaacaa gagatgaaac tggatccaac gccgattgct catcgtaccc
      541 atggccttaa ccctaataat ctcaataaat atgatgcgcg cattgcggcg attgattaca
      601 cccttgccca cgatgacggc atttcgttgc gcaatctgga tcaggctcag gtgatcctgc
      661 tcggtgtttc tcgctgtggt aaaaccccca ccagtctgta tctggcaatg caatttggta
      721 tccgcacggc aaactacccc tttattgccg acgatatgga taatctggtg ctacccgcgt
      781 cgctcaaacc gcttcagcat aaattgttcg gcctgactat cgacccggaa cgtctggcgg
      841 cgattcgcga ggaacgtcgg gagaacagtc gctatgcctc gcttcgtcag tgcagaatgg
      901 aagtcgcgga agtggaagcc ttgtaccgta aaaatcagat cccgtggatt aacagtacca
      961 attattcggt agaagagatt gccaccaaga tcctcgatat catgggcctt agtcgccgaa
     1021 tgtactagag aactagtgcg ctggtttgtt ttttgttatc atgctaacca cccggcgagg
     1081 tgtgacacac ctcgcacttg aaatcagctg cgattggttt atcgtgatgc gcatcacttc
     1141 ccggtagtcc tgccgtagaa gcaacaaatt tctgagactt gtaatgaaca gaactgacga
     1201 actccgtact gcgcgtattg agagcctggt aacgcccgcc gaactcgcgc tacggtatcc
     1261 cgtaacgcct ggcgtcgcca cccatgtcac cgactcccgc cgcagaattg aaaaaatact
     1321 gaatggtgaa gataagcgac tgttggtcat tattggcccc tgctcgatcc acgatctcac
     1381 cgctgcaatg gagtacgcca cccgtctgca gtcgctgcgc aaccagtacc agtcacggct
     1441 ggaaatcgta atgcgcacct attttgaaaa accacgaact gttgtcggct ggaaagggct
     1501 aatctccgat ccggatttaa acggcagcta tcgggtaaat cacggtctgg agctggcgcg
     1561 caaattactt ttacaggtaa atgagctggg tgtccctacc gcgactgagt ttctcgatat
     1621 ggtgaccggt cagtttattg ctgatttaat cagttggggc gcgattggcg ctcgtactac
     1681 cgaaagtcag atccaccgcg aaatggcttc agcactctcc tgtccggtag gttttaaaaa
     1741 tggtaccgat ggcaacacgc ggattgccgt ggatgctatc cgcgcagccc gcgccagcca
     1801 tatgttcctc tcgccagaca aaaatggtca gatgaccatc tatcagacca gcggcaaccc
     1861 gtatggccac attattatgc gtggcggcaa aaaaccgaat tatcatgccg atgatatcgc
     1921 cgcagcctgc gatacgctgc acgagtttga tttacctgaa catctggtgg tggatttcag
     1981 ccacggtaac tgccagaagc agcaccgtcg ccagttagaa gtttgtgagg atatttgtca
     2041 gcaaatccgc aatggctcta cggcgattgc tggaattatg gcggaaagtt tcctgcgcga
     2101 aggaacgcaa aaaatcgtcg gcggtcagcc gctcacttac ggtcaatcca ttaccgaccc
     2161 gtgtctgggc tgggaagata ccgaacgcct ggtcgaaaaa ctcgcctctg cggtagatac
     2221 ccgcttctga atgcgtgccc attcctgatg ggatgggcat ttctgcgcaa cttgttgtct
     2281 tctcaacaca ttactgcttg ctctggcctg ccataatatt gataataaga atcattgtta
     2341 tatcaattat tattaatttt tatgcgttat acggatagca gaaaactcac gcctgaaacg
     2401 gatgccaatc acaagaccgc ttccccgcag cccatccggc gaattaccag ccagacactg
     2461 ttaggtccgg atggcaaact gattatcgat catgacgggc aagaatatct gctccgtaaa
     2521 actcaggctg gcaagctgct gttaactaag tagcctttaa ctcgagcagc tgacttccag
     2581 ccgtttaccc cagtcaggtg gacgactgac atagtcatca tctctgtcgc tgaaaggatt
     2641 tcgcaacgcc tcatgcaggc ggtgcaattc catcatatca cccttttctg ccgcttcaat
     2701 cgcccgttgc gccaaccagt tgcgcaatac cagagctggg ttaacgcttt gtatcagttg
     2761 ctgacgctca ctatcgctaa cctcgtcttg ttgcaaacgc ccccgataac gggcaaacca
     2821 gtcatcaaat gccgcacgat caataaactc atcacgtagc ggtgacgccg cgctgtgctg
     2881 ctcggtcaga ctcagcatgc ggaatgtgcg ggtataatcg ctgcgctctc gcgccatcag
     2941 actgaataat tcattcagta gcgcgttatc ctctttttgc tccgtcatga agcccagttt
     3001 ctgccgcatc cgttgtccat aatgcgtcaa caaaacctgc tgatagctgt ccagggcctc
     3061 attcagggca tctacggcaa caaatggtga caatgtctgc gccagacgct gtaaattcca
     3121 caacgcgaca gcaggttgat tatcaaagct ataacgccct tgatgatccg agtgattaca
     3181 aataaaaccg ggttcgtaat catcaagaaa accaaacggc ccgtaatcaa gcgtcagtcc
     3241 cagcagcgac atgttgtcgg tattcatcac cccatgtgca aagccgaccg tctgccattg
     3301 ggcaattaac gacgcggtac gtgcgacaac atcgctaaac cagagacggt atttgtcctc
     3361 atcatcttca agatgtgacc agtaatgacg aatagcaaag tcagctaact gacgaacctt
     3421 ttccgactcg cggcggtagt aaaaatgttc gaaatgacca aagcgcagat gacttggtgc
     3481 cacacgcatc agcatcgcgc ctggctccgc cgtttcccga tacactggcg aatcgctggt
     3541 gacgatactt aacgcgcggg tcgtcggaat gcccagataa tgcatcgcct cactggcgag
     3601 actttctcgt atcgtcgaac gtaaaaccgc ccgtccatca cccattcgcg aataaggcgt
     3661 caggccagca cctttcagat gccagtccat tgtagtgcca tcagcaagca gttgttcgcc
     3721 gagtaaaatg ccgcgcccat cacccagttg gcccgcccag acgccgaact gatgaccact
     3781 gtaaacctgc gccagtggtg acataccagg cagtagggct tcgccgcccc agacacctgc
     3841 gccattttta aacagcgacg atggaatact cagcgtgtta gccagttcga tattatgcca
     3901 aatcagccgg gcattattta aaggcgtagg ggaaagtgct gtataggttt ctggcaattc
     3961 atcgcgccag cgggtaacaa aagacagggt catagatcct cctgtttgat agtgtagacg
     4021 gttactctcg tcttaaacac cagcaaacag aagggttatc gctgaaccaa cgtcgttatc
     4081 tgatttatgg gaacggcagg ccacaggcat ccctggagag catggaaatc gaagggggta
     4141 atctgtgcca atatttctgc cgtatcaatg cctcccgcaa tgatgcaatt gcaacaaggg
     4201 gaaatttgcg cctggatcgc acgaataaac ggttcgaagg agcggtgcgt gatttgttgc
     4261 tgaataaaac ttttatccag catgacgcgg gtgaaaagac catcaaaaac cgctttcatt
     4321 gtgctattgc ctgctcccaa attacctaac acaaggggat acacttgcga taaggataaa
     4381 agaccgcgat tatcttttcc ttcattgaga tgcgggtaat tctcattaat caacagttca
     4441 ataaacggat attttaatag ttcgcctgcg taattatccc tctctaataa taatgtagcc
     4501 acttgtggcg ttaaattaag ccaggcaaag agtttatgtt gaataaaaaa atgttggcat
     4561 gattttagta attccaactg ctctgaaaat aattgccaat gttgctcctc ggtaagttgc
     4621 gctataaccc gactcgtcgg gatacgcacc gtgccatctt cactggagaa atgggtaatt
     4681 aactcaacgc cgacaaggac ttgctggtta tctcttatcg gcaggaaata gcaatcagaa
     4741 tgataaagat tctccaaaaa aatcttcatg gtaaccgtcc ctctcgaagg atgtttttca
     4801 gtatccggct aaaagggatg aggccataag acataacaat tacagaagga gtaactttca
     4861 tttgttccat gttaaccact ttttcagggg tcttttttta gattatcctg attataaacg
     4921 aataatccat ttacggaatt tttgtctgca aaatactact gtattcagga gtaaatggac
     4981 ggcgatgtat catcgccgtc catttttcga catactagat tcgtctcgcc tgccagaaat
     5041 ttttctgcca atagacatta tcaagtgagg aacgcatcac tcccttgctg gtagaggcgt
     5101 ggataaattg gttgttggta tcgtaaatac ctacatgcaa accattttgt ccggaccccg
     5161 ttttgaaaaa gaccaggtca cctggcagca acttgtcttt atcaatttgc gtgccaatag
     5221 aggcttgttg tttggtttca cggggcagct gcaaatcgaa acgatcgcgc atcgtcacaa
     5281 ccacaaatcc cgaacagtcc acaccgcgcc gcgtcatgcc accataacga tacggcgtgc
     5341 catgccagct ttgtagctgg tcgttcaaac cggcaataac ggtaatcgaa tcagaaagtc
     5401 tggcatttgg cggcggtgct ttatggtggc tacacccggc cagaaacagt gctgtgatca
     5461 aaataaggca gaaacgcatt ccgtacggtt cctctgtttt ttattcttgc attaatttag
     5521 cgtcgtaatt acccgatttt caagatacta atgaaatcag atggtcaaaa tcagcattct
     5581 gtgaccttcg atatccagac ggcgaaaatt catcccatag gcctgcgcca gatttggcgg
     5641 cgtgagcacc tcttctctgc gtccactggc cagcattttt ccacctttta gcaaccacgc
     5701 ccgatgcgca tgacgcaacg tgtggttgag atcgtgactg ctcatcacaa tcgccagtcc
     5761 ttgctgacac agcgcgctca gaattttgtc taacgcactt tgttgcgcaa catcaagact
     5821 gttcatcggc tcatcaagaa gcagcaattg gcctgcggga ttggcttgtg gtgtgatttg
     5881 caacaccacc gcagcaagac ctacgcgttg ccattcaccg ccggaaagtt gattggtgct
     5941 acgaccgagt ttgtcatcaa gagccagcgc ccctgcgaca tcattcagta gttcggtacg
     6001 cgttttatcg tgctgatgca gtgtcaggta gtgccagacc ggcattgcaa acggcggcgt
     6061 ctgctgttgt gaaagatagg cgcgatgcag cgcgagtttt gttgcggacc atgcttccag
     6121 gggttgcccc gcgaactgaa tgcttccctt accgctggtc attccggcca ttcgtgcaag
     6181 caaggtactc ttacccgcgc cattcggccc caccaggtgc aggatctccc cagcccgaac
     6241 ctcgccagaa agtggcccta ggcgggtaga ttccgcaaca tcctgtaact gcatcacaat
     6301 agacattatt ttgccaacgc cagtttaatg ctttccatca caatggggtc ttccggcgtc
     6361 atatccgggg aaaaacgctg gatgaccaat ccgtccctgc caactaaaaa tttctcaaaa
     6421 ttccataaaa tatcatcagg gtacagcggt gcacgacctt tgctgaccat acgggcatag
     6481 aatccgctct cttccggcgc gactgcggtc ggcgctgcgg caatcaattt ttgatacagc
     6541 ggatggcgtc cttcgccgtt aacttcaatc ttactgaaca tcgggaacgt caccccccat
     6601 gtggtggtac agtaagtttt aatctcttca tcgctgcccg gttcttgttc cagaaactgg
     6661 ttgcacggga atcccagcac cacaaaacct cgatcggccc aggctttctg aatattctcc
     6721 aactgctcat attgcggcgt taagccacac tttgaggcga cattgacaat caacagcaca
     6781 ttaccggcgt acttctccag cgtggtcact tcaccgtcga tatctttcac tacggtcgtc
     6841 agaatggaat cttgcatcgt ttctcctggg tgtggtcagt aaaaatctta gcttttaatc
     6901 atagaccgtc tttttgcggc taacgtcttg cttttaacaa taaccagata aacaccggcg
     6961 cacccaacgt tgcggtgacc acgccaataa gcagctctgc ggcagctaat gccaggcgcg
     7021 ctacaatatc ggccagcagc aatgcgctcg cccctgccag cgcgcagccg ggaagtaata
     7081 cgcgatgatc ggttaaacca cacaaccgaa gaatatgtgg gatcaccaga ccaataaagc
     7141 cgatagcacc cgccagcgcc acactgacgc caaccatcca gccggtcgcg gccaccagca
     7201 cattgcgcca gaaccacagg ggtaaaccca gttgccgcgc cgagatctcg ccaagtgcta
     7261 acatattcat cggcctggac tgacaacaga tccacaacaa cacggggatc aatgccagca
     7321 tcagccagct ttgccgccag tctacgccgc caaaaccgcc catcatccag tacatcagct
     7381 gacgtaaatc aacagaggtg gaaaagtaga tagcccacgt cattagtgcg ctacagataa
     7441 tccctaatgc aacgccagcc agcaataacc gactggtcga aagatgacga cgggcgaaac
     7501 gtaagagtat taaagtaatg ataagcgcgc cagcaatcgc acacagccct agcgcccagt
     7561 tggggagttg cccttgccca agcaataccg cggcgataag ccccacgcct gcgccgttag
     7621 agacgccaag tagtccaggt tctgccagag gattttcaaa caacgcctgc attacagcgc
     7681 cggatatagc cagcgccgca ccaaccaaca atacagccag cgtacgtggc aggcgaattt
     7741 gccagacgaa cagttcgcca cgaggagtaa accagtcacc tagcgagatc cattgttcac
     7801 cggcgcaaag gcttaaaaga agcgccagca gcatcaaaac tgacaggcat aataaccagc
     7861 gaatattttg tcgctgttgt tggcgggcaa gtgtcagcat ggtatccgtt ctgctgaagt
     7921 gtcatggcgt tgattttacg gtgactcttc gacagtgaaa agaaaaaagg ccgcagagcg
     7981 gccataaaca caagtaaaaa tcaacaagtt agataattat caaagactta cagacacaca
     8041 aaaacacagc caaccacaac aaataacagg gatgtggtca ctttgtggat catatcgccg
     8101 ccataaattt acttaataac agtaaccaca gccacaagca cggccatcgc agacaggaca
     8161 atacctgtga gtaaccaagt ctgatttgca aatgatttct gaagttcagt acgatgctca
     8221 gccatctcaa ctttcagaga ctggcgtaaa tcagccatct cagacttcag ggattggcgc
     8281 gattctgcca tctcaagcct caacccttcg cgaatctcca gtacatcaga tttggtagca
     8341 aaggattcgc ttcttgtggt gagagtaatc aggttgtttt tgatttcagt cacatcactt
     8401 tccaggcaac tgacccgccg ttcaaggtcg tcattcatgc catcacctcc atcattacca
     8461 cttcctgtta cttggttgtt ggatacatta taggcattct ctttcttttt tctgaaccgc
     8521 gaaatatgtg caacattctc tgccatcact tctcatcacc ctgcttgcct acccagttca
     8581 taaaggcccg ggccgagtaa cgatgaacaa acccacatct gttacaggta agtcggaatt
     8641 catagttatg gatattttcc cgatcatgtc cctcatatcc cggataccca gtaaatggac
     8701 cgatgtaatc aagcataaca aaagtgccta aatcacactc ctcagcagtt ttcggctcag
     8761 gccccacctc aacaatattc gtcaaataca tgtacatatc cgtatcgcca cacgacaaac
     8821 atttttcatt cgtagacgac tcattgagaa aacgcgcaaa attatcgagc gttgccattc
     8881 ttttaagttc atctaaatga aacttcactc ttaagcacct cctagtggat taaacctcac
     8941 cgcatcctgc aggtaatccg gcgcaagatg ggcataaatc atcgttgtct gaatctttgc
     9001 gtgccccaga attttctgga gcgtcagaat attgccgccg ttcat
//
LOCUS       DAZPEB010000159         8927 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZPEB010000159 DAZPEB010000000
VERSION     DAZPEB010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8927
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.162"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(246..1217)
                     /locus_tag="ACHURJ_003276"
     CDS             complement(246..1217)
                     /locus_tag="ACHURJ_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family plasmid partition
                     protein"
                     /protein_id="HFR1282104.1"
                     /translation="MKRAPVIPRHTTHSQSTEDTSSPAPAAPMVDSLIARVGAMARGN
                     AISLPVCGREVKFTLEVLRGDSVESASRVWSGNERDQELLTEDALDDLIPSFLLTGQQ
                     TPAFGRRVSDVIEIADGSRRRKAAILTESDYRVLVGELDDEQMAALSRLGNDYRPTSA
                     YERGLRYTSRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFAHPGELSA
                     RSGEALQKAFADKEELLKQQAETLHDQKKAGLIFEAEEVISLLTSVLKQSPASRVNLS
                     SRHQFAPGATALYKGDKMVLNLDRSRIPAECIEKIEAILKELEKPGV"
     gene            complement(1217..2383)
                     /gene="sopA"
                     /locus_tag="ACHURJ_003277"
     CDS             complement(1217..2383)
                     /gene="sopA"
                     /locus_tag="ACHURJ_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid-partitioning protein SopA"
                     /protein_id="HFR1282105.1"
                     /translation="MGLMDTLNQCISAGHEMTKAIAIAQFNDDSPEARKITRRWRIGE
                     AADLVGVSSQAIRDAEKAGRLPHPDMETRGRVEQRVGYTIEQINHMRDVFGTRLRRAE
                     DAFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDASYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPA
                     DPHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQ
                     FFDMLRDLLKNVDLKGFEPDVRILLTKYSNNNGSQSPWMEEQIRDAWGSMVLKNVVRE
                     TDEVGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     gene            3124..4134
                     /locus_tag="ACHURJ_003278"
     CDS             3124..4134
                     /locus_tag="ACHURJ_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017900946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFR1282106.1"
                     /translation="MTSENNSLLLNLQEVDKTTGEVVKLDVNSTSTVQPVALMRLGLF
                     VPTLKSTGKSKANRKNVTDATEELVQLAIAKSEGYTDVKITGSRLDMDTDFKVWLGII
                     RSMSEYGVKSDTLELSFVEFVKMCGFDSRRSNKKMRDRISNSLFKLASVTLKFQSETK
                     GWTTHLVQSAYYDINEDIVEIKAEPKLFELYHMDRRVLLRLKAIDALQRKESAQALYT
                     YIESLPQNPAPISMKRMRDRLNLTSNVYTQNHTVRKAMEQLRDIGYLDYTEFKRGRAT
                     YFSVHYRNPKLISSPVKVPRKEEEEKAPEQNYDEVIKALKAAGIDPLKLAEALSAMKP
                     EN"
     gene            complement(4946..5269)
                     /locus_tag="ACHURJ_003279"
                     /pseudo
     CDS             complement(4946..5269)
                     /locus_tag="ACHURJ_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224879.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
     gene            5387..6112
                     /locus_tag="ACHURJ_003280"
     CDS             5387..6112
                     /locus_tag="ACHURJ_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFR1282107.1"
                     /translation="MAYSIGEFARLSGITATTLRAWQRRYGLLKPQRTEGGHRQYSDE
                     DVQQALKILDWVKKGVPIGQVKPLLERPTSRLTNNWLTLQQSMLQRLQDGKIESLRQM
                     IYDAGREYPRPELVANVLRPLRSQVSANVAAAMTLREILDGIIIAYTSFCLEGDKKAP
                     GDNILLSGWNLNDPCEVWLEALTRTGQGHRIDILPTPPATLAPEIFPDRHWLLVTRGK
                     LTAARKKQIEQWQQQVSLEVIIL"
     gene            6212..6622
                     /gene="uraH"
                     /locus_tag="ACHURJ_003281"
     CDS             6212..6622
                     /gene="uraH"
                     /locus_tag="ACHURJ_003281"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033971 - hydroxyisourate hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006144 - purine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFR1282108.1"
                     /translation="MKKTASLLMLTTLAFAPAAFSAPAGTLSVHILDQQTGMPPSDVT
                     VTLEKQQQDKWTPIASGKTDHDGRIKSLYPEDQDMQPGVYKVAFKTADYFHGKKLDSF
                     FPEIPVLFTVTRTNEKLHIPLLLSQYGYSTYKGS"
     gene            6725..7684
                     /locus_tag="ACHURJ_003282"
     CDS             6725..7684
                     /locus_tag="ACHURJ_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFR1282109.1"
                     /translation="MTTKPVLGISGCLTGSAVRFDGGHKRMGFVMDELAQWVSFRPVC
                     PEMAIGLPTPRPAIRLTLTDSGETQLRFSKPPHDDITQKMADFTADYLPKIGDLSGFI
                     VCAKSPSCGMERVRLYDENGNRGRKEGVGLFTAALLETYPWLPVEEDGRLHDPVLREN
                     FIERVFALHELNTLRAKGLTRRALLDFHSRYKLQLLAHHQAGYREIGPFVASLHEWED
                     LDAFFVAYREKLMTILKKPASRKNHTNVLMHIQGYFRNQLNSRQRVELRDVILHYRDG
                     LLPILAPLTLLKHYMAEYPDRYLMTQNYFDPYPDDLGLRLAVT"
     gene            7830..8918
                     /locus_tag="ACHURJ_003283"
     CDS             7830..8918
                     /locus_tag="ACHURJ_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR1282110.1"
                     /translation="MITTLHIQNYRSIREMSLELEQLNIVFGPNGTGKSNIYKAIHLM
                     HSAAQGQFSQALANEGGILKVFWAGKTRSDQLRRMNLAVETETYEYELQVGFVEKLPY
                     PSQFQLDPVIKEESIWLSGQYRRPSSQLMKRKNQAVFLNNVHHEKVTHSGTLYENESV
                     FGQLGEPHLYPEVSQMRESLRNWRFYQEFSVSIGSAMRAPQVGFRSPVLASDGANLAA
                     AFQTIVEIGDELLLMRILDQAFPGCVFYSDNTGGRFRMMMQREGLSRPLEPAEFSDGT
                     LRFLCLAVALLSPRPPAFIALNEPENSLHPQMLPALASLIAEASRYSQIWLTSHSPEL
                     ANLIEKQRSFSLYQLSMVEGETKVERLG"
BASE COUNT         2255 a         2359 c         2287 g         2026 t
ORIGIN      
        1 ggcgatgcag gtgggaccgt ggtcccaggc agataacctg accggcgata caggtgggac
       61 cgtggtccca ggcagataag ctgaccgacg acgcaggtgg gaccgtggtc ccagggagat
      121 aacctgagcg gcgatacagg tgggaccgtg gtcccagaga gaatattcag gccagttatg
      181 ttttctggcc tgtgagaaag gaaatgaagt aaagggagat caccgtagac taaagcgtgt
      241 ccgcatcaga ctcctggttt ctcaagctcc ttaagaatgg cttctatttt ctctatacac
      301 tccgcaggaa tgcgggacct atccaggtta agcaccattt tatcgccctt atacaatgct
      361 gttgcccctg gagcaaactg atgacgtgag ctcagattaa ctcttgatgc gggagattgt
      421 ttcagtacgg atgttaaaag actaatgacc tcttcagctt caaagattag tcccgctttc
      481 ttctgatcgt ggagagtctc agcctgctgc ttcagtaatt cttctttatc tgcaaaagcc
      541 ttctgaaggg cttcacctga ccgggcagac agttctccag gatgtgcaaa cagggcaaca
      601 acggatttag gcaatttggc cgtattgata cagcgggtaa tgatcttacg agagatgttt
      661 tccgcatcgg caagcgcaga aatatttcct gcaaactcat tctgcagccg gcttgtataa
      721 cgcaggccac gttcataagc actcgtcggc cgataatcgt tacccagtcg ggacaatgca
      781 gccatctgct catcgtccag ttcaccaacc agaacgcgat aatcactttc cgtcaggatt
      841 gcggctttac ggcgacggct gccgtcggca atttctatga catcagaaac ccggcggccg
      901 aaagctggag tctgctgacc ggtcagtaaa aatgaaggaa tgagatcatc cagagcatct
      961 tcggtaagta gttcctgatc gcgctcattg cctgaccata cgcgtgaagc gctctcaaca
     1021 gagtctcccc ggagcacctc aagggtaaat ttcacttccc gtccacatac cggaagagag
     1081 attgcattac cgcgggccat agcacccacg cgcgcaatta atgaatccac catcggcgcg
     1141 gccggcgccg gtgatgaagt atcttcagtc gattgactat gtgtggtatg tcttggaatg
     1201 acaggagcac gcttcattat ctaatctccc agcgtggttt aatcagacga tcaaaaatct
     1261 cattgcaaac aggctcccag atagagaggg cattacgcca ggcaccggtt gaagaacgtt
     1321 gatcaatagc ctgttcaaaa acggttctca tacggatctg acctttaccg acttcatccg
     1381 tttcgcgcac aacattttta aggaccatgc ttccccaggc atcccgaatt tgttcttcca
     1441 tccacgggga ctgtgagcca ttattattac tgtatttggt aagcagaata cggacatctg
     1501 gctcgaagcc tttaagatcc acgttcttaa gcaggtcacg aagcatatcg aaaaactgca
     1561 gtgcggaggt gtagtcgaac agctctgccg gagtagggac aatcagaaca tcagcagcgc
     1621 atacaacatt tatcgtaccg ataccaaggt tgggtgcact gtcgataact atcacgtcat
     1681 aatcatgagc gacagtttca atggccagac ggagcatcag gtgcgggtca gcaggtaatt
     1741 tgccttcgtc aaatttcccc atcagctcgg tttcaatgcg gtgcagtgcc agacaggacg
     1801 ggatgatatc aaggccaggc cagcaggtgg gctttatagc gtagctggcg tcatcctttt
     1861 ccccaagata aaagggcagg agagtgtcct cagcatgaat atgaagatcg ggcacccatc
     1921 cgtgatacat agaagcagtt ccctgtgggt cattgccttc aacaagcaga acacggagtc
     1981 ctttcagagc aagatcctga gccaggtgaa cggatactga ggttttgtaa acaccacctt
     2041 tgtgagctgc aacgccaatc accggcggaa acgcatcttc agcacggcgc agtcgtgtac
     2101 caaatacatc ccgcatgtga ttgatttgtt cgatggtata accaacgcgt tgctcaactc
     2161 tcccacgggt ttccatatca gggtgcggca gccggccagc tttctcggca tccctgatag
     2221 cctgagatga cacgccaact aaatctgccg cttcaccaat cctccagcgc cgggttattt
     2281 tccgggcttc cggactgtca tcattaaact gtgcaatggc gattgctttc gtcatttcat
     2341 gaccagcact tatgcactgg ttgagtgtat ccatgagtcc catactgaac atcctttaat
     2401 catcactttg cattatttta ttaaatcttg gaatttattg caaagcaaca ataaaatcgc
     2461 aaagcacacg aaaaagtgca aagtgaacca cattgggtaa tgaaagaacg caggggatta
     2521 caaaagagta cataccgcag tcaatattaa tcactgccgc tcgccgcagt aagcgatctc
     2581 acacagtgag aaagcggcga ggaagcggaa aatcacagaa ctcacagatt gcacgtacgt
     2641 cgggttgcta aagatgactc gggatcagcc agttataatg aaattgactg cggtatgtaa
     2701 tatattgact atggtatgat gcttaccgca tctgtaggaa agacgactca gcataccgca
     2761 gtcaatgttt cattgttgta cctgtagatc tcaatcttcc agagcgtatt caggaaaatc
     2821 aggccagggg tctggatatc tggtgtggcg cttccggcct tgtatctggg ttattcatgg
     2881 tctcgatcca ataattgatc ccaaggagat catagtgaga tccaaaagag atccccggat
     2941 cgctccagca tgcgctgtaa ctggctttaa cgtctgtctg gatcgactgc ggaatgtaaa
     3001 atgttgactg ttatatgcat aatctaatac tgtgatatgc aacggtattt actgaggtat
     3061 gtatatccgt tgactgcgat acgtattact acatcctttt gtggataacc gttgaggatc
     3121 aagatgacga gcgaaaataa tagcttactt ctgaaccttc aggaagttga taagacaacc
     3181 ggtgaagttg ttaagctgga tgtcaacagc accagtaccg tgcagccagt agcgctcatg
     3241 agactcggtc tcttcgtccc aactctgaaa tcaacaggga agagcaaggc gaaccggaaa
     3301 aacgttacag acgcgactga ggagcttgta cagctggcca ttgccaaaag cgaaggatac
     3361 actgacgtta agatcaccgg ttcgcgtctt gatatggaca cggattttaa agtctggctg
     3421 gggataattc gctccatgtc ggagtatggg gtaaaaagtg acaccctgga actgtcgttc
     3481 gtcgaattcg ttaagatgtg cggatttgac tcccgtcgtt caaacaaaaa aatgcgcgat
     3541 cgcatcagta attccctgtt taaactcgcc tcggttacgc tgaagttcca gagcgagacc
     3601 aaaggatgga ccacccatct ggtgcagtca gcctattatg acatcaacga ggatatcgtt
     3661 gagatcaagg ctgaacctaa gctgtttgag ctttaccaca tggacagaag ggtactgctg
     3721 cggctgaaag ccattgacgc cctgcagcgc aaggaatcag cccaggcgct ttatacctac
     3781 atcgaaagtc ttccccagaa tcctgcacct atttccatga aacggatgcg tgaccggctg
     3841 aatctcacct caaatgtcta tacacagaac cacacggtgc gtaaggcgat ggagcagctg
     3901 cgtgatattg gttatctgga ttacaccgag tttaaacgtg gccgagccac ctatttcagc
     3961 gtgcattatc gcaaccccaa actcatcagt agtcctgtga aggttccgcg caaagaggaa
     4021 gaagagaagg cgccagagca aaattatgac gaagtcatta aggcgctgaa ggctgctggc
     4081 atcgatcctc ttaaactggc tgaagccctc tcagcgatga agccagaaaa ttgactgcga
     4141 tatgcatatc atgactgccg tatgccgcta aggacattcc attgactgtg atatgcatca
     4201 cgcataccgc agtcaatatt tattctctat catgtctgcc cggatcgaac aatattgact
     4261 gtcatatgtg atttcgggtc atgatacgca gatattgact acggaatgcg ggtttaatgg
     4321 gggtaatccc tggtctgttc accattccat gggggataac atgccacagt caatttttca
     4381 gtaacgctct atatccctta cgaaaatctc aatcgggtgc tgagacggtc atgttacctg
     4441 acggtatccg ttcccctgtg tactgcaggt ttagcccggc aggatgagac tgcagctgtc
     4501 tgtataaccc ggcgccagct tcacactgtt aaatgcgatc aggaaacggg agccgaatgg
     4561 cagaaaggtg ttggctaggg aaggtgcgaa taagcaggtt atttcttccc aagctgactc
     4621 gctgattaaa atttcgcgga tctgggccga tttttttccc gcaaacacat cgaatcagcc
     4681 tatttagggg tcagtttgga gatcggaaat ttttgtacgt taagcacatt atttaaccaa
     4741 ttcggatacg taagtgattg atttatcgtc attggctatc gcttttaggt tgtcgctctt
     4801 gctgtatcgc atttaggtta tccgattaag acaaaatccc ctgaaaacgc tgtatagcgc
     4861 gggagtacac cctattgcta caggtaagcc tgttcaaaaa acaggcttac cgtacaataa
     4921 ttctctatat ccaaactgac cccaagaagg acaccttcag cgcggccgtg gtcggttcgc
     4981 cggtaaagta ggctttaccc tcgctctcat tccacttgtt cttcacaggg tcgtagccgc
     5041 ggttaagcac gctgatgccg ccgtcgctgc gcttgccgta ggtggcggta acctgctcta
     5101 ggccgcgttc aaagcggttt tccaggcgag cgacttcgta ccatttgcca agatagcggc
     5161 tggcgtcaaa accggaaatg ggctgcacgc ccttcggtgg ggtaggggat ttacaggcta
     5221 ccagcgtcag ggcaatggca acaccggtca caacaggcca tagcttcatg agaattcctt
     5281 tcatttgcac ggttggaaat taagtgtagt gcaccgcggc ggcgcttcat cccatccgcg
     5341 aaaattcgta gtatattgag agtattcctc aactctggac acggacatgg cttactccat
     5401 cggcgaattc gccagactca gcgggatcac cgccaccacg ctgcgggcct ggcagcggcg
     5461 ctacggctta ctcaagcctc agcgcacgga gggcggccac cgccagtaca gcgatgagga
     5521 cgtccagcag gcgctaaaaa tcctcgactg ggtaaaaaaa ggcgtcccga ttggccaggt
     5581 caaaccgctg ctggagcgcc ctacgtcgcg cctgaccaac aactggctca cgctgcagca
     5641 gagcatgtta cagcgcctgc aggacggcaa aatcgagtcc ctgcgtcaga tgatttacga
     5701 cgcaggacgc gagtacccaa gaccagaact ggtcgcgaac gtgctgcgtc cgctgcgcag
     5761 tcaggtgtcg gcaaacgtcg ccgccgccat gaccctgcgg gagatcctcg acggcatcat
     5821 catcgcctac acctcgtttt gcttggaagg cgataaaaaa gcgccgggcg acaacatcct
     5881 gctcagcggc tggaacctga atgacccgtg cgaagtctgg ctcgaagcct taacccgcac
     5941 cgggcaaggc caccgtatcg atattttacc cacgccgccc gccacgctgg ccccggagat
     6001 tttcccggac cgccactggc ttctggtcac gcgcggtaaa ctcaccgccg cgcgcaaaaa
     6061 gcagatcgag cagtggcagc agcaggtatc gcttgaagtg attatcctct aatcattttc
     6121 tcctgcggga atcttcacga aaagttgaat attttcccgc gccacaaatg ctaacgtttt
     6181 cctgtgaccg acaaccccac aggaaaacac aatgaaaaaa actgcctctc tgctgatgct
     6241 gaccaccctg gcctttgccc ctgccgcctt tagcgcccct gccggcacgc tgagcgtaca
     6301 cattcttgac cagcaaaccg ggatgccgcc atcggacgtt accgtcacgc tggaaaaaca
     6361 gcagcaggac aaatggaccc caattgccag cggcaaaact gaccatgacg gccgcatcaa
     6421 atcgctttat ccggaggatc aggatatgca gcccggcgtc tataaagtgg cctttaaaac
     6481 cgcagattac ttccacggga agaagctgga ctccttcttc ccggagatcc cggtgctgtt
     6541 caccgtaacc cggaccaacg aaaagctgca catccctctc ctgctgagcc aatacggtta
     6601 ttcaacttac aagggcagtt aagaaacgtt gattttaaag gcaaataaaa aataattccg
     6661 ctaatacttg gacaaattag ttatgttgtg taagttttaa agagaagaac gggaggacca
     6721 caccatgaca acaaaacctg tgctcggtat tagcggatgt ttgaccgggt ctgccgttcg
     6781 cttcgacggc ggacacaagc gtatgggctt tgtcatggat gaactggccc agtgggtgag
     6841 tttcaggccc gtctgcccgg aaatggcgat tggcctgccg acgccacgcc cggccatacg
     6901 cctgacgctg acggacagcg gcgaaacgca gctccgcttc agcaagccgc cccatgatga
     6961 tataacgcag aaaatggccg actttacggc ggactatctg ccaaaaatcg gcgatctgtc
     7021 gggatttatc gtctgcgcga aatcgccaag ctgcggcatg gaacgcgtgc ggctatatga
     7081 tgaaaacggc aaccggggcc gcaaggaggg tgtcgggctg ttcaccgccg ccctgcttga
     7141 aacctacccg tggctgcccg tggaggaaga cggtcggctg cacgacccgg tgctgcggga
     7201 aaactttatc gagcgggtat ttgccctgca cgagctgaac acgctgcgcg ctaaaggcct
     7261 gacgcgccgc gcgctgctgg atttccacag ccgctataaa ttgcagcttc tggcgcacca
     7321 tcaggcgggc taccgcgaga ttggcccgtt cgttgcgtcg ctgcatgaat gggaagatct
     7381 ggacgcgttc ttcgtggcgt accgcgaaaa gctgatgacc atcctgaaaa agcccgcctc
     7441 gcggaaaaac cataccaacg tgctaatgca tatccagggc tatttccgta accagctgaa
     7501 cagccgccag cgcgtcgagc tgcgcgatgt gatcctgcac tatcgcgacg ggctgctacc
     7561 catcctcgcg ccgttgacgc tactgaaaca ctacatggcc gaataccctg accggtatct
     7621 gatgacgcaa aactactttg atccctatcc tgatgatttg ggtctgcgtc tggcagtaac
     7681 ctgacgataa aaatgcgaag gcccgtttga cccacagagg cccaaagcag taccccgttt
     7741 agacgacacc ctcactgtcg tctctttttt gcattccaca caaaagactg taattatgta
     7801 caggattgta acgcaaaaag ggactgcctg tgatcaccac cctgcacatt caaaactacc
     7861 gctcgattcg cgaaatgtcg ctggagctgg aacagctcaa catcgtcttt ggcccaaacg
     7921 gcaccggaaa atccaatatc tacaaggcga tccacctgat gcacagcgcc gcacaggggc
     7981 agttttcgca ggcactggcg aacgagggtg ggattttaaa agtgttctgg gcaggcaaaa
     8041 cccgcagcga ccagctgcgg cggatgaatt tggccgttga aaccgaaacc tacgaatacg
     8101 agctgcaggt aggctttgtc gaaaaactgc cttacccctc acagtttcaa ctcgatccgg
     8161 tgatcaaaga agagtccatc tggctgagtg gtcaataccg ccgcccgtcg tcacagctga
     8221 tgaagcgtaa aaatcaggcc gtatttctga acaacgtgca tcacgagaaa gtcacccaca
     8281 gcggcacgct gtatgagaac gaatcggtgt tcggccagct tggcgagccg catctttacc
     8341 cggaagtgtc gcagatgcgc gagtcgctgc gtaactggcg cttttatcag gagttttctg
     8401 tatcaatcgg ctcggccatg cgcgcgcctc aggtcggttt ccgctcccca gtgctggcca
     8461 gcgacggcgc aaacctggcg gcggcgtttc agaccatcgt ggaaattggc gacgagctgc
     8521 tgctgatgcg catcctcgac caggccttcc ccggctgcgt gttttacagc gacaacaccg
     8581 gcggacgctt ccggatgatg atgcagcgtg aagggctaag cagaccgctg gagccggcgg
     8641 aattctccga cggcactctg cgttttctgt gcctggcggt ggcgctatta agcccgcgtc
     8701 caccggcctt tatcgccctc aacgaaccgg aaaacagcct gcatccgcag atgctgcccg
     8761 cgctggcgag cttaatcgcc gaggccagcc gctactcgca aatttggctg accagccact
     8821 cgccggaact ggcgaattta attgagaaac agagatcgtt ttcgctgtat cagctgtcga
     8881 tggtggaagg agaaacgaag gtggagaggc tggggtagtc tcccaga
//
LOCUS       DAZPEB010000160         8829 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZPEB010000160 DAZPEB010000000
VERSION     DAZPEB010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8829
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.163"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            124..744
                     /gene="fklB"
                     /locus_tag="ACHURJ_003284"
     CDS             124..744
                     /gene="fklB"
                     /locus_tag="ACHURJ_003284"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR1282111.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            1053..2465
                     /gene="cycA"
                     /locus_tag="ACHURJ_003285"
                     /gene_synonym="dagA"
     CDS             1053..2465
                     /gene="cycA"
                     /locus_tag="ACHURJ_003285"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFR1282112.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFLG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWTGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(2510..3172)
                     /gene="ytfE"
                     /locus_tag="ACHURJ_003286"
     CDS             complement(2510..3172)
                     /gene="ytfE"
                     /locus_tag="ACHURJ_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFR1282113.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYNMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(3280..4245)
                     /locus_tag="ACHURJ_003287"
     CDS             complement(3280..4245)
                     /locus_tag="ACHURJ_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR1282114.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNTQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLIRQQMPPLMTLSGIALLVVGVVIVVRAKPEKPLTESVSES"
     gene            complement(4353..5213)
                     /gene="qorB"
                     /locus_tag="ACHURJ_003288"
     CDS             complement(4353..5213)
                     /gene="qorB"
                     /locus_tag="ACHURJ_003288"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFR1282115.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            5302..5682
                     /locus_tag="ACHURJ_003289"
     CDS             5302..5682
                     /locus_tag="ACHURJ_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFR1282116.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDEHAA"
     gene            complement(5811..7754)
                     /gene="cpdB"
                     /locus_tag="ACHURJ_003290"
     CDS             complement(5811..7754)
                     /gene="cpdB"
                     /locus_tag="ACHURJ_003290"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFR1282117.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEEADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDRHIAFASPDE
                     NRSVLTAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            7944..8684
                     /gene="cysQ"
                     /locus_tag="ACHURJ_003291"
     CDS             7944..8684
                     /gene="cysQ"
                     /locus_tag="ACHURJ_003291"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFR1282118.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
BASE COUNT         2056 a         2228 c         2301 g         2244 t
ORIGIN      
        1 acgggatatt gctattgttt ctttttcccc gggatttgtc atcattcctg tgacaaagtt
       61 ttacccaaag aagtgtggct gatatgctgc ccgctacttt tataccctaa gaaaggaaat
      121 acgatgacca ccccaacttt tgacaccatc gaagcgcaag caagctacgg cattggtttg
      181 caggtagggc aacaactgag tgaatctggc ctggaagggc tgctgccaga agcactggtt
      241 gcaggtattg ccgatgcgct ggaaggcaaa catccggctg ttccggttga tgtggtgcat
      301 cgcgcgctgc gtgaaatcca cgaacgcgcc gatgccgttc gtcgtcagcg tttccaggcg
      361 atggctgctg aaggtgtgaa atacctggaa gaaaacgcca aaaaagaagg tgtgaatagc
      421 accgaatctg gcctgcaatt ccgcgtgatc aaccagggtg aaggcgcaat tccggcacgt
      481 accgaccgcg ttcgtgttca ttacaccggt aaactgatcg acggcaccgt gtttgacagc
      541 tccgttgctc gtggtgaacc cgctgaattc ccggttaacg gcgtgatccc tggctggatt
      601 gaagcactga ctctgatgcc ggtaggttct aaatgggaac tgactatccc gcaggaactg
      661 gcatatggcg agcgcggcgc aggtgcatcc atccctccgt tcagcaccct ggtgtttgaa
      721 gtcgaactgc tggaaatcct ctaagcagcg cattctgttc ccctcgaacc agaggggagc
      781 aggcattcag caataaaccc ttcagtttgc caaacggcgc tattttgtgt tgcaaagacc
      841 ccgtaagcgt gtatttttgt gagatgtttc gcgttatcac cgtgatatga cactcacttt
      901 aaacataaaa ttaacattag atctaaatct tagtattcat cccgcgtatt gttacctaat
      961 atcgatgagt cccgatacag attcgtcgta tcatagactg actaaaggcc gtagagcctg
     1021 aacaacacag acaggtacag gaagaaaaaa acatggtaga tcaggtaaaa gtcgttgccg
     1081 atgatcaggc tccggctgaa cagtcgctac ggcgcaatct cacaaaccga catattcagc
     1141 ttattgccat tggcggtgcc attggtacgg ggttgtttct ggggtctggc aaaacgatta
     1201 gccttgccgg gccgtcgatc attttcgttt atatgatcat tggttttatg ctctttttcg
     1261 tgatgcgggc aatgggggaa ttgctgcttt cgaatctgga atacaaatct tttagtgact
     1321 tcgcttccga tttactcggg ccgtggacag gatatttcac tggctggact tactggttct
     1381 gctgggttgt aaccggtatg gcagacgtgg tggcgatcac ggcttatgct cagttctggt
     1441 tccccgatct ctccgactgg gtcgcctcgc tggcggtgat agtgctgctg ctgacgctca
     1501 accttgccac cgtgaaaatg ttcggtgaga tggagttctg gtttgcgatg atcaaaatcg
     1561 tcgccatcgt gtcgctgatt gtcgtcggcc tggtcatggt ggcgatgcac tttcagtcac
     1621 cgactggtgt ggaagcgtca ttcgcgcatt tgtggaatga cggcggctgg ttcccgaaag
     1681 gtttaagtgg cttctttgcc ggattccaga tagcggtttt cgctttcgtg gggattgagc
     1741 tggtaggtac aacagctgcg gaaaccaaag atccggagaa atcactgcca cgcgcgatta
     1801 actccattcc gatccgtatc attatgttct acgtcttcgc gctgattgtg attatgtccg
     1861 tgacaccgtg gagttcggta gtcccggaga aaagcccgtt cgttgagctg tttgtgttgg
     1921 taggtttgcc tgcggctgcc agcgtgatca actttgtggt gctgacctct gcggcgtctt
     1981 ccgctaacag cggcgtcttc tctaccagcc gtatgctgtt tggtctggcg caggaaggtg
     2041 tggcaccgaa agcgttcgct aaactttcta agcgcgcagt accagcgaaa gggctgacct
     2101 tctcctgcat ctgcctgctc ggtggcgtgg tgatgctgta tgtgaatcct agcgtgattg
     2161 gtgcgttcac gatgattaca accgtttccg cgattctgtt tatgttcgtc tggacgatta
     2221 tcctttgctc gtacctggtg taccgcaaac agcgtcctca tctgcatgag aagtcgatct
     2281 acaagatgcc gctcggcaag ctgatgtgct gggtatgtat ggcgttcttt gtgttcgtgg
     2341 tcgtgttgct gacactggaa gatgacactc gccaggcgct gctggttacc ccgctgtggt
     2401 ttatcgcact ggggctgggc tggttgttta ttggtaagaa gcgggctgct gaactgcgga
     2461 aataaccgca ttatcatgct ggatggcgca atgccatcca gcttttagat cactcacccg
     2521 ccagcgcgcg tgggaacagt acattgtttt ccagactgat gtgatccatc aggtcatcaa
     2581 tcagttcatt aatgccgtta tacatcgctt tccaggtggt gcaggcttct ggcggcggtg
     2641 tgacgttatt ggtagtgtgt ttgatcactt ccagcagttc gcccgcttca tcgtgctcgc
     2701 tttccattac gctgattggc cccattgcct ggctgcccat gccttgtttg atcatcggga
     2761 agaggatctg ctcttctttc atcatgtggc tggaaagctc ttcatgcagc atggtcaggt
     2821 attttgtcag cccttttggc acgctcggtt tgtcggcgtg aacgcgctcg actttagtcg
     2881 cttgcaggat cagctccggc agttgctcgc ggtgacgatc gtggtagcgc acgatgatat
     2941 ggtcaatgat ttctgccagc ggggcgctac gccagtcttt ctcaatcggt tgttcagcaa
     3001 gctttgccag ttcagcttca atgacctcaa catccagttc tttacgtgcc gccgcgcgcg
     3061 ccagcgtctg cttaccgcca cagcagtaat ccatattata tttacgaaac agagctgaag
     3121 cgcgaggaat agagagcgcc agttcaccta aaggttggtc gcgataagcc atagctgata
     3181 cctcattctt aataagataa gatgtatttt aaatacatct ttaaggcaaa agctataacc
     3241 cttacgtagc agaggcttat ttaactcaca gccagtatgt caactttctg agacagattc
     3301 agttaaaggc ttttccggtt ttgctctgac tacaatgacc acgccaacca ctaacagcgc
     3361 gataccgctc agcgtcatca gcggcggcat ttgctggcgt atcaaaaagg tgtacagcaa
     3421 acctgccagt gtttcgaaaa cgatcagcgg cccgagaatc actgtcggta atcgctggct
     3481 ggcgacgttc cagcagagtg cgccaaccca tgagcaaagc acggctatcg caaccatcag
     3541 actaataaac accagcggac gggggccaaa aggtagggag aagtccggcg tttgcgtatt
     3601 cagccagtaa caggcgacga gatagccgat gagagaaacc ggcagtgtga ccagcgcctg
     3661 cgccgtcgcc cacatcatcg gatgtttgtc gggattctcc cgcagccagc gggcgttgcg
     3721 cagggcatac catgcccagc agaccacgga aactaacgct agcacgatgc ctgaggtata
     3781 acgtgcccag tcaaaatcgg ggagtccgtg gtttaactca gcaatattca cacacgccag
     3841 gccgatgcca atacaaacca gtgccggggc gagttttccc cacgcgagtt tgccgtcgcg
     3901 ctggctataa agcagattgg caaaaacggg aatcaccacc ggcagggtgc cgataatcat
     3961 cgtggaaaca ggcgcaccag tacgttgaat ggcactggca aggcagaagt aatagatgag
     4021 gttgcccatc atagtgagca tcaaggcggt aagccagtcg cgacgcgcca actgacgcag
     4081 acgcacgcgt cccagccagg caatgggcag cgcaattaac cctaacgcca gataacgccc
     4141 catcgactgc aacatcgccg ggtattccgg tacgatcaac ggcccgacaa aaataagccc
     4201 ccacatcaac cctgctaaca gggcgtacag cacgccgcta atcattactg gcatccattg
     4261 gtctgtcaga agaaagttca ggaagcagac atgattgctc ccggcatccc gtcattatta
     4321 gagaggatgc gggaggatgc cactttaatt aactagttat taacattaaa aagatggctt
     4381 acgctttcgg ctaacgttgt cgttgggcgg ccaatcaatt tgctaagcgt tttgctgtta
     4441 tcaaacagac cacctttcga tgcgccaacg tcagaatccg ccagcatatc cgccagtccg
     4501 tcgggcagtc cgacgctttt cagtgccgcg gcgaaatcgg cttcgctcag attttgatag
     4561 gtaacctgtt tgccgctctg tttggtcagc tctgccgcta actgtgtcaa cgtccaggca
     4621 ctatcgcccg ccagttcgta aaccttgcct tcgtgaccgg cttcgctaat cacgcgtgcc
     4681 gcagctgccg cataatctgc ccgcgttgct gaggcgattt tgccatcgcc cgccgcaccg
     4741 ataaatacgc cgtgttccag tgctgccggg gcgctggcga ggtagttttc gctgtaccag
     4801 ccgttgcgca gcagggtgta aacgatgcca gaatcagcca acattttctc cgtctcaatg
     4861 tgctcatcgg cgaggccgag cggggaggta tctgcatgta gcaggctggt ataagcgata
     4921 aatttcacgc cagccgcctt tgcggcatta ataacattac gatgctgcgg ggcacgttga
     4981 cccacttcgc tggaagagat cagcagtagt ttttccactc cctgaagtgc agatgtcagt
     5041 gcggcttcat cgccgtagtc agcctgacgc acggtaatgc cttgtgctgc cagagcctgg
     5101 gcttttgccg gattacgaac gatagccact atttggctgg caggaaccgt tttcatcaag
     5161 gattcaataa tatagtgacc aagttggcca gtggcaccag taatagcgat catgggaagt
     5221 ctccatcgtt ttgtcttgtg tttaaatacg ctaacaccta aacttacttt tagtaagtac
     5281 gtacaaaaag gtaagtatga aatgagtcag gttagcctgt cgcaacaact gaaagagggt
     5341 aatctctttg cggaacagtg cccgtcgcgc gaggtgttga aacacgtcac cagccgttgg
     5401 ggggtgttga ttctggtggc gctacgcgaa ggtactcatc gctttagcga cctgcggcgc
     5461 aaaatcggcg gggtgagcga aaagatgctt gcgcagtcgt tacaggcgtt agaacaggat
     5521 ggttttctta accgtatcgc gtatccggtg gtgccaccgc atgtggaata tagcctcacg
     5581 ccgctgggcg agcaggtgag cgaaaaggtt gctgcactgg cggactggat tgagttgaat
     5641 ttgcccgagg tgttggcggt gcgggatgaa catgcggcat aactgccgga tgcgctgagc
     5701 ttatctggtc tacggtgcat ggctggtagg gcggataaga tgcgccagca tcgcatccgc
     5761 caataaatgc cggatgcggt tgataattta tagaccgaaa aagaagtgtt ttacttactc
     5821 aaatccacct gataaatcgc aaacccgata tcatcggtcg cgactttatt catcggatat
     5881 tgccctttct ctttaataaa cgctgcggct ttatctgacg gggaagtttc gaaacggata
     5941 tccagtttct tatcgccagc tatcggtgct aaacgccagt tgttatctgc cgccgggtga
     6001 atttctcccg cacgtttcga ctcatcagca atccacgctg tcagcaccga gcggttctca
     6061 tccggtgaag caaaagcgat atgtctgtcg cccgtaccgg caaatttgcc gccgtaagcg
     6121 cgatagttat tggtggcaac gaggaacata gcgttcggat caatcggctt gccattaaag
     6181 gtcaggttct taatccgctc tgcattggca ttaatcatct ggcactcgcc gtcatagcgg
     6241 gcgggttggg taacatcaat ctgataattc acaccatcaa taacatcaaa gttataagtg
     6301 cggaaaccat cccagttgat gagtgactgt ggtttcgtgc tgttaggatc aatctggtta
     6361 aactgtccgg cggagcactc cagccactct ttcacctctt taccgctggc tttcactaca
     6421 atcagcgtat tggggtagag ataaagatcg gcggcattac ggaaggtcaa ctggcctttt
     6481 tccacctcca catagcttgc cgggtcattt ttgcgaccac caactttaaa cggtgcggca
     6541 gctgaaagca ccggcagttt tgccagatcc ggatcgccct gaatgtaatg ttcgacatac
     6601 gctttttgcg cgttgttcac gacttgcacg gtcggatcgt cctgcaccag cgccagatag
     6661 ctatacatat tgtcggcaga tttaccgatt ggcttgctga cgaactggcg tgtggcatcg
     6721 tgatcggctt tgagtgtttc taccagcttg ctgtcttccg ccgcgaggga ttttttattg
     6781 gcgatgtcgt aaatcggccg tgcttccgct ttcgcctgcg tcacctgcca tttaccgctg
     6841 tcattactga gttgtaagtc gaccacccca agatgatcgc cccacatgcc cggcattacc
     6901 gccggaacac cattcagcgt gcctttggtg atatcagcct cttcgatatc agcaaaatct
     6961 ttacccggga aaacggcgtg agcatggcca aacataatgg cgttaacgcc cggaatttca
     7021 ctgaggtaat aaactgagtt ttccgccatt actttatacg gatcggcaga cagcccggaa
     7081 tgcgccagaa cgacaacgac atcggcacct ttctcgcgca tttcaggcac gtatttgcgc
     7141 acggtttcgg taatatcatt caccgtcact tttccggata aattagcttt atcccagccc
     7201 atgatttgcg gcggcacgac gccaatatag ccaatcttca gcgtctgttt ttttccgtct
     7261 ttatcgacca cttcggtatc tttaattaaa tacggtgtaa acattggctg tttggttctg
     7321 gcgtcaatga cgttggcatt tacataaggg aatttcgctc ccgccagcgc atttttcagg
     7381 taatccagac cgtagttaaa ttcatgattg ccgagtgtac cgaccgtata atccagcgta
     7441 ttcagcgcct tataaaccgg atgaacatca cctgctttta atcctttcgc cgacatgtaa
     7501 tcggccagcg gactcccctg aatcaaatcg ccgttatcga ccagtacgct gtttttcact
     7561 tcattgcggg catcgttaat caggcttgcc gtacgtacca gtccgaattt ttccgtggcg
     7621 gtgtctttgt aataatcgaa atccatcatg ttgctatgca gatcagtggt ttccatgata
     7681 cgcagatcga ccgtcgctgc attcacactg gcggcaatca gcgtggccag gagcgttgcg
     7741 ctaaacttaa tcatcaggga catcctttta tcatcgggaa tacgaaagaa aagggagaat
     7801 aaacgtctta cttatagaac agtgaagaat gccacaattt tacgctttga aaatgatgac
     7861 actatcacag ttggcgcatt cattaacgat agggtataag taaaacaata agttaacacc
     7921 gctcacagag acgaggtgga gaaatgttag atcaagtatg ccagcttgca cggaatgcag
     7981 gcgatgccat tatgcaggtc tacgacggga cgaaaccgat ggacgtcgtc agcaaagcgg
     8041 acaattctcc ggtaacggca gcggatattg ccgcacacac cgttatcatg gacggtttac
     8101 gtacgctgac accggatatt ccggtccttt ctgaagaaga tcctcccggt tgggaagtcc
     8161 gtcagcactg gcagcgttac tggctggtag acccgctgga tggtactaaa gagtttatta
     8221 aacgcaatgg cgaattcacc gttaacattg cgctcattga ccatggcaaa ccgattttag
     8281 gtgtggtgta tgcgccggta atgaacgtaa tgtacagcgc ggcagaaggc aaggcctgga
     8341 aagaagagtg cggtgtgcgc aagcagattc aggtccgcga tgcgcgcccg ccgctggtgg
     8401 ttatcagccg ttcccatgca gatgcggagc tgaaagagta tctgcaacag cttggcgaac
     8461 atcagaccac gtccatcggc tcttcgctga aattctgcct ggtggcggaa ggacaggcgc
     8521 agctgtaccc gcgcttcgga ccaacgaata tttgggacac cgccgctgga catgctgtgg
     8581 ccgcagctgc cggagcgcac gttcacgact ggcagggtaa accgctggat tacactccgc
     8641 gcgagtcgtt cctgaatcct gggttcagag tgtctattta ctaaattcag atggcagaaa
     8701 cagtgtattt cctgattctg ccatcctgat ttctcccaac ctaaaaagtt ataaataaaa
     8761 agagattgta tttaaagtgc aaaaattcaa ttgctaataa gttacatttt aataatgagc
     8821 gttttttga
//
LOCUS       DAZPEB010000161         8734 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZPEB010000161 DAZPEB010000000
VERSION     DAZPEB010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8734
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.164"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            244..1155
                     /gene="ttdA"
                     /locus_tag="ACHURJ_003292"
     CDS             244..1155
                     /gene="ttdA"
                     /locus_tag="ACHURJ_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFR1282119.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            1152..1757
                     /gene="ttdB"
                     /locus_tag="ACHURJ_003293"
     CDS             1152..1757
                     /gene="ttdB"
                     /locus_tag="ACHURJ_003293"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFR1282120.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRGKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            1806..3269
                     /gene="ttdT"
                     /locus_tag="ACHURJ_003294"
     CDS             1806..3269
                     /gene="ttdT"
                     /locus_tag="ACHURJ_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFR1282121.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVFGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(3312..4325)
                     /gene="tsaD"
                     /locus_tag="ACHURJ_003295"
     CDS             complement(3312..4325)
                     /gene="tsaD"
                     /locus_tag="ACHURJ_003295"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFR1282122.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            4562..4777
                     /gene="rpsU"
                     /locus_tag="ACHURJ_003296"
     CDS             4562..4777
                     /gene="rpsU"
                     /locus_tag="ACHURJ_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFR1282123.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            4888..6633
                     /gene="dnaG"
                     /locus_tag="ACHURJ_003297"
     CDS             4888..6633
                     /gene="dnaG"
                     /locus_tag="ACHURJ_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFR1282124.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            6828..8669
                     /gene="rpoD"
                     /locus_tag="ACHURJ_003298"
     CDS             6828..8669
                     /gene="rpoD"
                     /locus_tag="ACHURJ_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFR1282125.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         2109 a         2330 c         2334 g         1961 t
ORIGIN      
        1 cctgcaagtc tttggctaaa ggccagctat tcagcatcgt taattatccg cagttgtgat
       61 aagcgcagtg tatttcgcaa aacattgcca ttccctgctc tggcgcaata ttcattctta
      121 ttttcgcgtt gattcacgaa ctgttaatta catattcacg gtggcaaaaa atataaaacc
      181 acatttttag tggtagttta tggcggtgaa tttttccaga caaatacaaa aactggagtt
      241 gccatgatga gcgaaagtaa taagcaacag gcagtgaata agttgacaga gattgtcgct
      301 aactttaccg ccatgatttc tacccgaatg cctgatgacg tggtggataa actaaaacag
      361 ctaaaggatg ccgaaacgtc gtcgatgggg aaaattatct accatacgat gttcgacaac
      421 atgcaaaaag cgattgacct gaatcgtcct gcctgtcagg acaccgggga gattatgttc
      481 ttcgttaaag tcggttcccg cttcccactg cttggcgagc tgcaaagcat actcaaacaa
      541 gccgtggaag aggcaaccgt caaagcgcca ctacgtcaca atgcggtaga aatttttgac
      601 gaagtaaaca ccggcaaaaa taccggtagc ggcgtaccgt gggtcacctg ggacatcatc
      661 cccgacaatg acgatgcgga aatcgaagtt tacatggcag gtggcggctg cacgctacct
      721 ggccgctcga aagtgttaat gccgtcagaa ggctacgaag gcgtggtgaa attcgtcttc
      781 gaaaatatct ccaccctcgc cgtaaacgcc tgtccaccgg tactggtggg cgtgggcatc
      841 gccacctcgg tggaaaccgc cgccgtactc tcgcgtaaag ccattttgcg cccgattggc
      901 agccgccatc ccaatccaaa agcggcagaa ctggagctac gcctggaaga aggactcaac
      961 cgtctgggga ttggtccaca agggctgact ggcaacagtt cagtgatggg cgtacatatc
     1021 gaatctgccg cccgccatcc gtcaaccatc ggcgttgctg tctctaccgg ctgctgggcg
     1081 catcgtcgcg gcacactgct ggttcatgcc gatctcacct ttgaaaatct gtctcacacc
     1141 cggagcgcgt tatgaaaaag atcctgacaa ccccgatcaa agctgaagat ctgcaagata
     1201 ttcgcgtcgg cgatgtgatc tacctgaccg gtacgctggt gacctgccgt gacgtttgtc
     1261 accgccgctt gatcgaactg aaacgtccga tcccttacga tctcaacggc aaagcgattt
     1321 tccacgctgg ccccatcgtg cgcaaaaacg gcgataaatg ggagatggtt tccgttggcc
     1381 cgacaaccag tatgcgtatg gaaagttttg aacgtgaatt tatcgagcag accggcgtga
     1441 aactggtggt tggcaaaggc ggaatggggc cgctgaccga agaaggctgc cagaaattca
     1501 aggcgctaca tgtgattttc ccggcaggct gcgcggtgct ggcggcaacc caggtggaag
     1561 agattgaaga agtgcactgg acagagctcg gaatgccgga gtcgctgtgg gtctgccggg
     1621 gcaaagagtt cggcccgctg attgtctcta ttgataccca cggcaacaat ctgatagccg
     1681 aaaacaaaaa gctgttcgcc gaacgccgcg atcccatcgt ggaagagatc tgcgagcacg
     1741 tccattacat caaataaccc tcccggagag gctcgccccc tctccttttt cgcaggcata
     1801 acacgatgaa accttccact gaatggtggc gataccttgc gccgctggcg gtcatcgcca
     1861 ttattgctct aattccggtt cccgcagggc tggagagtca tacctggctc tactttgccg
     1921 tttttactgg cgtgatcgtt ggactgatcc tcgaacccgt gccgggtgcc gtggtggcga
     1981 tggtgggtat ctccatcatc gccatactct ctccctggct gctgttcagc cccgaacagc
     2041 tcgcccagcc aggctttaag ttcaccgcca aatccctctc ctgggcagtt tttggttttt
     2101 ctaattcggt tatctggctc attttcgccg cctttatgtt tggcacaggc tatgaaaaaa
     2161 ccgggcttgg acgccgcatt gcgctgattc tggtgaaaaa gatgggacat cgcacgctgt
     2221 ttctcggcta tgcggtgatg ttctccgagc tgatcctcgc acctgtaaca ccgtccaact
     2281 cggcgcgtgg tgcggggatt atctatccca tcatccgtaa cctgccaccg ctctatcaat
     2341 cacaaccaaa cgacagcagt tcgcgcagca ttggctcgta catcatgtgg atggggattg
     2401 ttgccgactg cgtgaccagc gccattttct tgacggcgat ggcaccaaac ttgctgttaa
     2461 ttggactgat gaaaagcgca tctcacgcca cgctgagttg gggcgactgg ttcctcggga
     2521 tgttgccgct cagtattttg ctggtgctgc tggttccctg gctggcttat gtgctgtacc
     2581 cgccggtact gaagtctggc gatcaggtgc cgcgctgggc agagacggaa ctgcaggcaa
     2641 tgggcccgct ctgttcgcgt gaaaaacgga tgctggggct gatggtaggc gcgctggtgc
     2701 tgtggatttt cggcggtgat tatatcgatg ccgcgatggt cggttacagc gtggtggcac
     2761 tgatgctgct tctgcgcatt atcagttggg acgacattgt cagtaataaa gcggcgtgga
     2821 acgttttctt ctggctggcc tcgcttatca ccctcgctac cggactcaac aacactggtt
     2881 ttattagctg gtttggcaaa ctgttagcag gcagcttaag cggttattcg ccaacgatgg
     2941 tgatggtggc gttgattgtg gtgttttatc tgctgcgcta ctttttcgcc agcgccacgg
     3001 cgtatacctc cgccctcgca ccgatgatga ttgccgctgc gctggcgatg ccggaaatcc
     3061 cgctgccggt gttctgccta atggttggcg cggcaattgg tctggggagc attcttacac
     3121 catacgccac cggacccagt ccgatttact acggtagtgg ttatctgcca acggcggatt
     3181 actggcgact gggggcgatt tttgggctga tattcctcgt gttgctggtg attaccggct
     3241 tactgtggat gcccgtggtg ttgctttaag tgaggacggt gccggaaatt gctctccggc
     3301 acccaatagt tttacgcagc cggtaactcc gccagcggcc agcgcggacg cacgctaacg
     3361 ccgagatccg ccgtcgcgcc tgctttaaac cgcaccattc cggcataggc gatcatcgcg
     3421 ccgttatcag tacaaaactc cggacgcgcg tagaacactt cgccgcggcg ttttttcatc
     3481 atttccgcca gcttcgcccg taacgtgcgg ttagcactca cgccgcccgc catgaccagt
     3541 cgcttaaagc ccgtctgatc caacgctcgc ttacacttaa tcatcaacgt atcgaccacc
     3601 gcatcttcaa aggcgcgggc gatatcagca cgcgtctggt cgtcggtgcc gttgtcacga
     3661 atcgtgttcg ccgcaaaggt tttcagacca gaaaagctga aatccagccc cggacggtcg
     3721 gtcatcggac gcgggaaaac aaagcgcccg gcagtaccct gagccgccat tttcgacagt
     3781 aacggtccgc caggataatc cagccccagc agcttcgcgg ttttatcaaa cgcttcaccg
     3841 gcggcatcat cgatagactc gccgagcagc tcgtactgac caatgccagt cacgctgatt
     3901 aactgcgtat gaccgccgga caccagcagc gcgacaaacg gaaattccgg cgggttatct
     3961 tccagcatcg gcgctaacag atgcccttcc atatggtgta ccgggattgc cggaacgttc
     4021 caggcaaacg ccagagaacg ccccacggtt gcgccaacca gcagtgcgcc gactaatcca
     4081 gggcctgcgg tataggccac agcatcaatg tcttttgccg ttaagccaga ctcctttagc
     4141 gccgcctgga tcaacggtac ggttttgcgc acgtgatcgc gggaggccag ttcaggcacg
     4201 acgccgccgt agtcagcgtg caatttcacc tgactataca attggttggc taacaaacct
     4261 ttttcatcgt cgtaaatggc gatgccggtt tcatcgcagg aagtttcaat acccagtaca
     4321 cgcatgactt gttttacctc gctttattac cgcgcagtgt aggaccaatg cgggttgatg
     4381 taaaactttg ttcgcccctg gagaaagcct cgtgtatact cctcaccctt ataaaagtcc
     4441 ctttcaaaaa ggccgcggtg ctttacaaag cagcagcaat tgcagtaaaa ttccgcacca
     4501 ttttgaaata agctggcgtt gatgccagcg gcaaaccgaa ttaatcaaag gtgagaggca
     4561 catgccggta attaaagtac gtgaaaacga gccgttcgac gtagctctgc gtcgcttcaa
     4621 gcgttcctgc gaaaaagcag gtgttctggc ggaagttcgt cgtcgtgagt tctatgaaaa
     4681 accgactacc gaacgtaagc gcgctaaagc ttctgcagtg aaacgtcacg cgaagaaact
     4741 ggctcgcgaa aacgcacgcc gcactcgtct gtactaattc cccgagagcg ttgctctccg
     4801 atcagaccga gttgtagttg taaggccgtg cttccgaaag gaatgcgcgg cttattttcg
     4861 tttatgaatt gctaaaaatc ggggcctatg gctggacgaa tcccacgcgt attcattaat
     4921 gatctactgg cacgcactga catcgtcgat ctgatcgatg cccgtgtgaa gctgaaaaag
     4981 cagggcaaga atttccacgc gtgttgtcca ttccacaacg agaaaacccc gtcattcacc
     5041 gttaacggtg agaaacagtt ttaccactgc tttggatgtg gcgcgcacgg caacgcgatc
     5101 gacttcctga tgaactacga caagcttgag ttcgtcgaaa cggtcgaaga gctggcagca
     5161 atgcacaatc ttgaagtgcc atttgaagca ggcagcggcc ccagccagat cgagcgccat
     5221 cagaggcaaa cgctttatca gttgatggac ggtctgaata cgttttacca acaatcttta
     5281 caacaacctg ttgccacgtc tgcgcgccag tatctggaaa aacgcggatt aagccacgag
     5341 gttatcgctc gctttgcgat tggttttgcg ccccccggct gggacaacgt cctgaagcgg
     5401 tttggcggca atccagaaaa tcgccagtca ttgattgatg cggggatgtt ggtcactaac
     5461 gatcagggac gcagttacga tcgtttccgc gagcgggtga tgttccccat tcgcgataaa
     5521 cgcggtcggg tgattggttt tggcgggcgc gtgctgggca acgatacccc caaatacctg
     5581 aactcgccgg aaacagacat tttccataaa ggccgccagc tttacggtct ttatgaagcg
     5641 cagcaggata acgctgaacc caatcgtctg cttgtggtcg aaggctatat ggacgtggtg
     5701 gcgctggcgc aatacggcat taattacgcc gttgcgtcgt taggtacgtc aaccaccgcc
     5761 gatcacatac aactgttgtt ccgcgcgacc aacaatgtca tttgctgtta tgacggcgac
     5821 cgtgcaggcc gtgatgccgc atggcgagcg ctggaaacgg cgctgcctta catgacagac
     5881 ggccgtcagc tacgctttat gtttttgccg gatggcgaag accctgacac gctggtacga
     5941 aaagaaggta aagaagcgtt tgaagcgcgg atggagcagg cgatgccact ctccgcattt
     6001 ctgtttaaca gcctgatgcc gcaagttgat ctgagtaccc ctgacgggcg cgcacgtttg
     6061 agtacgctgg cactgccatt gatatcgcaa gtgccgggcg aaacgctgcg aatatatctt
     6121 cgtcaggaat taggcaacaa attaggcata cttgatgaca gccagcttga acgattaatg
     6181 ccaaaagcgg cagagagcgg cgtttctcgc cctgttccgc agctaaaacg cacgaccatg
     6241 cgtatactta tagggttgct ggtgcaaaat ccagaattag cgacgttggt cccgccgctt
     6301 gagaatctgg atgaaaataa gctccctgga cttggcttat tcagagaact ggtcaacact
     6361 tgtctctccc agccaggtct gaccaccggg caacttttag agcactatcg tggtacaaat
     6421 aatgctgcca cccttgaaaa actgtcgatg tgggacgata tagcagataa gaatattgct
     6481 gagcaaacct tcaccgactc actcaaccat atgtttgatt cgctgcttga actgcgccag
     6541 gaagagttaa tcgctcgtga gcgcacgcat ggtttaagca acgaagaacg cctggagctc
     6601 tggacattaa accaggagct ggcgaaaaag tgatttaacg gcttaagtgc cgaagagcga
     6661 tcgggaagcc cccgacagcc gcactgagag gcagcggcaa atatataagt acgccctcgt
     6721 aattatcgtt ggcggtaaac aaccgttgga tttcagcgtt aacggctgaa ggacatcggg
     6781 tcaatcgccc aacaccaacc tcatgaaata agtgtggata ccgtcttatg gagcaaaacc
     6841 cgcagtcaca gctgaaactt cttgtcaccc gtggtaagga gcaaggctat ctgacctatg
     6901 ccgaggtcaa tgaccatctg ccggaagata tcgtcgattc agatcagatc gaagacatca
     6961 tccaaatgat caacgacatg ggcattcagg tgatggaaga agcaccggat gccgatgatc
     7021 tgatgctggc tgaaaacacc gcggacgaag atgctgccga agccgccgcg caggtgcttt
     7081 ccagcgtgga atctgaaatc gggcgcacga ctgacccggt acgcatgtac atgcgtgaaa
     7141 tgggcaccgt tgaactgttg acccgcgaag gcgaaattga catcgctaag cgtattgaag
     7201 acgggatcaa ccaggttcaa tgctccgttg ctgaatatcc ggaagcgatc acctatctgc
     7261 tggaacagta cgatcgtgtt gaagcagaag aagcgcgtct gtccgatctg atcaccggct
     7321 ttgttgaccc gaacgcagaa gaagatctgg cacctaccgc cactcacgtc ggttctgagc
     7381 tttcccagga agatctggac gatgacgaag atgaagacga agaagatggc gatgacgaca
     7441 gcgccgatga tgacaacagc atcgacccgg aactggctcg cgaaaaattt gcggaactac
     7501 gcgctcagta cgttgtaacg cgtgacacca tcaaagcgaa aggtcgcagt cacgctgccg
     7561 ctcaggaaga gatcctgaaa ctgtctgaag tattcaaaca gttccgcctg gtgccgaagc
     7621 agtttgacta cctggtcaac agcatgcgcg tcatgatgga ccgcgttcgt acgcaagaac
     7681 gtctgatcat gaagctctgc gttgagcagt gcaaaatgcc gaagaaaaac ttcattaccc
     7741 tgtttaccgg caacgaaacc agcgatacct ggttcaacgc ggcaattgcg atgaacaagc
     7801 cgtggtcgga aaaactgcac gatgtctctg aagaagtgca tcgcgccctg cagaaactgc
     7861 agcagattga agaagaaacc ggcctgacca tcgagcaggt taaagatatc aaccgtcgta
     7921 tgtccatcgg tgaagcgaaa gcccgccgtg cgaagaaaga gatggttgaa gcgaacttac
     7981 gtctggttat ttctatcgcc aagaaataca ccaaccgtgg cttgcagttc cttgacctga
     8041 ttcaggaagg caacatcggt ctgatgaaag cggttgataa attcgaatac cgccgtggtt
     8101 acaagttctc cacctacgca acctggtgga tccgtcaggc gatcacccgt tctatcgcgg
     8161 atcaggcgcg caccatccgt attccggtgc atatgattga gactatcaac aaactcaacc
     8221 gtatttctcg ccagatgctg caagagatgg gccgtgaacc gacgccggaa gaactggctg
     8281 aacgtatgct gatgccggaa gacaagatcc gcaaagtgct gaagatcgcc aaagagccaa
     8341 tctccatgga aacgccgatc ggtgatgatg aagattcgca tctgggggat ttcatcgagg
     8401 ataccaccct cgagctgccg ctggattctg cgaccaccga aagcctgcgt gcggcaacgc
     8461 acgacgtgct ggctggcctg accgcgcgtg aagcgaaagt tctgcgtatg cgtttcggta
     8521 tcgatatgaa caccgaccac acgctggaag aagtgggtaa acagttcgac gttacccgcg
     8581 aacgtatccg tcagatcgaa gcgaaggcgc tgcgcaaact gcgtcacccg agccgttctg
     8641 aagtgttgcg tagcttcctg gacgattaat cggtaggccg gatcaggcgt tacgccgcac
     8701 ccggcactag tccctctgca caaacgccac cttt
//
LOCUS       DAZPEB010000162         8697 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZPEB010000162 DAZPEB010000000
VERSION     DAZPEB010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8697
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.165"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            107..283
                     /locus_tag="ACHURJ_003299"
     CDS             107..283
                     /locus_tag="ACHURJ_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001452667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFR1282126.1"
                     /translation="MKRLNKLRGWRKVSRSAILREAVEQYLERQQFPVRKAKGGRQKG
                     EVVGVDDQCKEHKE"
     gene            502..744
                     /gene="yiiF"
                     /locus_tag="ACHURJ_003300"
     CDS             502..744
                     /gene="yiiF"
                     /locus_tag="ACHURJ_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HFR1282127.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(1074..2003)
                     /gene="fdhE"
                     /locus_tag="ACHURJ_003301"
     CDS             complement(1074..2003)
                     /gene="fdhE"
                     /locus_tag="ACHURJ_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFR1282128.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLDDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(2000..2635)
                     /gene="fdoI"
                     /locus_tag="ACHURJ_003302"
     CDS             complement(2000..2635)
                     /gene="fdoI"
                     /locus_tag="ACHURJ_003302"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFR1282129.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2632..3534)
                     /gene="fdoH"
                     /locus_tag="ACHURJ_003303"
     CDS             complement(2632..3534)
                     /gene="fdoH"
                     /locus_tag="ACHURJ_003303"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFR1282130.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3547..6597)
                     /gene="fdnG"
                     /locus_tag="ACHURJ_003304"
     CDS             complement(3547..6597)
                     /gene="fdnG"
                     /locus_tag="ACHURJ_003304"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(6010..6012),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFR1282131.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVANYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            6791..7624
                     /gene="fdhD"
                     /locus_tag="ACHURJ_003305"
     CDS             6791..7624
                     /gene="fdhD"
                     /locus_tag="ACHURJ_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFR1282132.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            7819..>8697
                     /locus_tag="ACHURJ_003306"
     CDS             7819..>8697
                     /locus_tag="ACHURJ_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HFR1282133.1"
                     /translation="MALGLTGCDDKKAETETPPPANSQPAAPAPEAPVAKAEAKPETP
                     AQPVVDEQAVFDEKMDVYIKCYNKLQIPVQRSLARYVDWLKDFKQGPTGEERTVYGIY
                     GISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNTINEMDKYYTQENYK
                     DDAFAKGKTLHQTFLKNLEAFEPVAESYHAAIQEINDKRQLAELKNIEEREGKIFHYY
                     SLAVMISAKQINNLISQEKFDVDAAMKKVSELETLVAQAKEADKGGMNFSFINSAGQY
                     QLEAKKYVRRIRDKVPY"
BASE COUNT         2069 a         2197 c         2294 g         2137 t
ORIGIN      
        1 ctctttagag taacgttggt tttttctgct cattattagc tccttctgat gccattctat
       61 ttcaggaagg agtgtccgtt aaactcaggc tacctcagaa gaggcaatta aacggctgaa
      121 caagcttcgg ggctggcgta aggtttcacg atctgcaatt ttacgcgaag cggttgagca
      181 atatctggag cggcagcaat ttccggtgcg taaagcaaag ggaggcaggc aaaaggggga
      241 ggtagttggt gttgatgatc agtgtaagga gcataaggaa tgaaaaaaat ttttcattct
      301 tgaatataaa aaaacagatg cccttattct ggtattaata caaggctgtt ttacttgaac
      361 ttataataac tgcaactgtt acatcgtatc tggaaaacgc ctcgcaaaaa taaaaaatga
      421 tgcgtaaatg agctttcttt aaagattcgg caagggtact tttctctcca tagattcaaa
      481 aatgatattg gcgagatatt tatgaactct ctggcaggta tagatatggg cagaatttta
      541 ctcgatttat cgaatgaggt gattaagcaa cttgatgatc tcgaggtgca gcgtaatctt
      601 cctcgtgcgg acctattaag ggaagcggta gatcaatacc tgataaatca atcgcaaaca
      661 gcaagaacca gtgttcctgg catctggcaa gggtgtgagg aagatggtgt cgaatatcag
      721 cgtaagctgc gcgaggaatg gtaatgggaa aaaatgatgt caatcagatt gctgacaacg
      781 tgcgcgttgt tcatgccgga tgcggcgtga acgccttatc cggcctacaa agtcatgcaa
      841 attcaatata ttgaagttta ttggtaggcc tgataagcgc agcgcatcag gcaatcatac
      901 gcttgtcatc agtgacatac ccataacctt atcgatgtgt tggagggaag aacgaggcaa
      961 acagacaatt gcggcatgat cttgaatggg taacgcggaa tacgagagat ggtgtcgtca
     1021 caccctatga gctgtaatcc gggcgaccag cgccacccgg aaaacgtcag agattactcc
     1081 ccttcacccg gaaacaggaa cgggttgatg gaactgcggg catagccttc ttgctccatt
     1141 cgcgcgtcca gtaccagaga agcgaggtca tctgccacgg cttcaacttt cggttctttt
     1201 tcctgataaa gaatcttcag gtaagtgccg cagtcatcgc agctttcggc tttaatcgcg
     1261 gcctgttcgt catccagcga ccagtaatgc agtttgccgc tctgttcaca gttgctgcat
     1321 tttacgcgca ctacgtgcca ttcggtttca cacaggttgc agtgcaggta acgcagaccc
     1381 tgagtggtgc caatttgcac catgctggac accggcatag aaccacaaac cgggcaatat
     1441 tgacgttgct cgccgtattc agcgcgggct ttgccgggga tcagattggc catctgcgcc
     1501 cagtagagcg acagtgcagc ccagataaac ggcgctttat cgctgctgac ggatgagaaa
     1561 tcagaggcaa acagtgcgct ggccatatct tccagctcct gagtcgatgc cttctccaga
     1621 ttctcaatca ctgccagcgc cgggccgctc atttcaggtt tcagctcagc aatcagcgcc
     1681 atcagcagct tttgccagtg cttatcacgc ggcagaacgt gaatatccag cgggggcttg
     1741 ccctgtgcgc tggcttcttt aatgcgcgcg gtcagatcca tctccagcgg atggtcgtac
     1801 agcaccactt cctgggcgtg ggcgataagc gcagcaaagc gcaggtaatc atccagcgga
     1861 ttattttctg ccagctcgcg cagacgctcg gcgcggcggt tgtataaatt cttgagccga
     1921 gggaacaata acggcggaat catatccgcc gtacgtttct cgctcgaacc cagctcatct
     1981 tgcgggatta tgcgaatact cattcagctt tcttttccgt tgtcttgcgg acctcacggt
     2041 accagcgcgg gtgatgtttc ttcgcccagg cgctggtaac ccatccttcc accatggcgg
     2101 taatcgtgcc tttcacccaa agggcggcgt agatatgcac catgataacc acaattaacg
     2161 ccactgcggc aaatgaatgc agcattaacg cgaatcggat caccgggatt gagaaagcag
     2221 gcgcaaaata aggacgccag ataatcacgc cgctcaccag cagcagaacc aggaaaataa
     2281 tcgccgccca gaaaacgcat ttctgaccga agttataacg cccggtgtca cctacttcct
     2341 cgttgacgac gatcttacga atattcttcg cccaaaagat atcatcccga ttgattaggt
     2401 tgtgatgcca gtaacggaaa aacatgatga tgaacgaggc aaacataacc acgccgacaa
     2461 acgggtgcag aattcgcgcc agctgcggtg tgcccatgat ttgcatcaac cagttgaagg
     2521 acgggaacaa aaagcccagc ccgctcaccg ccgctaggat gaagcagaag gcggtgatcc
     2581 agtggttgat acgttccggc gcggtgtagc gcacgatggt gtcacgtcgt ttcatttgcg
     2641 ctcctcgtct ttctcttcgt gcagattatt ctcttcctca tccgcacggt tcggaccgac
     2701 acccacgtag tggaagatac tggctgcgaa ggtagccgca aagccaacag ctgcgagcgg
     2761 tttccagatg cctttccaga atttcacggt ttcgctgatt tccgggttct ccggcaagcc
     2821 atgatacaga tttggcttgt cagcatggtg cagcacgtac ataacgtgtg taccaccgac
     2881 gcctgccgga tcgtacaggc ccgcattgtc gtaaccgcgg gttttcagct ccgccacgcg
     2941 ctcgctcgcc agcgttttca tcgactcttt cgtaccgaag tgaatcgcgc ccgttgggca
     3001 ggtcttcacg caggctggtt cttgcccaac caccacgcgg tcaacgcaca gcgtacattt
     3061 gtagacgcgg ttgtcttccg ggttgaggcg cggaatgtcg aacggacagc ccgcaatgca
     3121 ataaccgcag ccaatgcact gctcggactg gaagtcgacg ataccgttgg catactgaat
     3181 aattgcacct tccgccgggc acgctttcag acagcctgga tcggaacagt gcatacagcc
     3241 gtctttgcgg atcagccatt ccagtttgtc gttctgctcc acttccgaga agcgcatcac
     3301 cgtccacgat ttggcactta aatcattggg gttgtcgtac accccaatgt tattgccgac
     3361 ggtatcgcga atgtcgttcc actctgaaca cgccacctga caggctttac agccgataca
     3421 ggtggtgacg tcgatgagtt tcgccacttc ttcctggaag tcccgcgcct gaggcgcggg
     3481 ggtcagaccg ttagtcgcgg aacgacgaat gatatcttga gattgataag ccatatgtcg
     3541 tctccgttac accttttcca cattcacaag gaaggactta aactccggcg tctgcgtgtt
     3601 cgcatcacca acgaatggcg ttaaagtatt ggcaataaag ccttttttag ctacgccttc
     3661 atagccccag tgaatcggaa taccgatggt atcgatatct ttgccgttcg ctttcagcgt
     3721 gcgaatacgt ttggtcacca ccgctttggc tttgatatag ccacggttgg aggagacttt
     3781 cacggtatcg ccctgggcaa tgccaagttt attcgccagc gactccccga tttccacaaa
     3841 ctgctctggc tgcaaaatcg cgttcaacag cgcgtgtttg gtccagtagt ggaagtgctc
     3901 ggtcagacga taggtggttc cgacatacgg gaacttatcg gctttaccca atgcttcggc
     3961 gtcgtcttta aagatacgtg cagccgggtt cgagataacg tttggatgca gcgggttagt
     4021 tcccagcggc gtttcaaacg gctcgtagtg ttccgggaac ggaccttccg ccatcttatc
     4081 gagggcaaac agacgcccca tgccttcctg ctgcatgata aacggcccga cgccgctacc
     4141 cggaggcgct gcgctgtagt ccggaatatc ccagccggtc cacttagtgc cgtcccattt
     4201 cagcaactga cgcttcggat cccacgggtt accctgcgga tctgcggagg cgcggttata
     4261 cagaatgcgg cggttaagcg gccatgccca tgcccagccc agcgtgttac cgaggccaga
     4321 cggatcggcg ttatcacgac gcgccatctg gttgccttcc ggcgtccagc taccggcgaa
     4381 aatccagcag ccacaggagg ttgtaccgtc atcgcgcaat tgggcgaacg agctaagttg
     4441 ttggcctttc ttgacgataa ccgcaccggt tgccggatcg gtaatatcgg ccagcgcctt
     4501 accgttgctc tccatcgcta cttcttccga cttcggctca tgcggaatgg cgtagttcca
     4561 ggtcatgttc agcacctgat ccgggttcgc gccaccctgt tcggcataca tcttgcgcaa
     4621 gcgcaggaag ataccggaga ggatctcacc gtcagtcagc gcaatccccg gggcgtccgc
     4681 acctttccag tgccactgca accagcgacc tgagttaacg attgaaccgt tctcttctgc
     4741 gaagcaggtc gatggcagac ggaacacttc ggtctggatc ttcgacgagt caacttcgtt
     4801 cagctcaccg tggttctgcc agaagttaga ggtttcagtg ttcagcgggt cgatggtgac
     4861 gaggaacttc agtttcgaca gacaaccgat cactttgttt ttgttcggga atgaggcaac
     4921 agggttaaaa ccctggcaga tatagccatt gaccttgccc tctttcatca tctcgaagta
     4981 ttgcaggacg tcgtagcctt tatcccactt cggcaaccag tcaaagcccc agctattttc
     5041 cgccgtcgct ttatcaccaa agaaggcctt catcatagag acgaagaatt tcgggtagtt
     5101 gccccagtag tttacctggc cttccagcag cggttttggc gtgttggcgg taaggtaggt
     5161 ttgcagatcg gtctgcttct cgcttggcag cgtcatgtaa cctggcaggc tctgcgacag
     5221 cagccccagg tccgtcagcc cctgaatatt ggagtgaccg cgcagggcgt taacgccgcc
     5281 gcctgccatc cccatattgc cgagcagcag ctggatcatc gccatcgtac gaatgttttg
     5341 cgcaccaacg gagtgttgcg tccagccgag ggcatacagg aatgaggcag ttttatcgtg
     5401 agcactggtt tctgcgatgt attcgcagac tttcaggaac gcgtcttttg gcgtaccaca
     5461 gatgttttca accacatctg gcgtgtagcg ggaaacgtgc tgtttcagca agttccacac
     5521 gcagcgcgga tgttgcagcg tggtatcgcg tttggcgaag ccgttttcgt ccagttcgta
     5581 agtccaggag gatttatcgt acttgcgttt ttccgcgtca tagccggtga aaaggccatc
     5641 ttcaaagccg taatcctcac gcacgatcag gctggcgttg gtataggctt cggtgtattc
     5701 gcggttgaat ttttcattgt tcagcaggta cagcaatacg cctgacagga aagcaatgtc
     5761 agtaccggaa cgaatagggg catagtagtt agctaccgcc gccgtacgcg taaagcgagg
     5821 atcgatcaca atcagcttcg cgccgttgtg aattttggct tccatcgccc agcggaaccc
     5881 gaccgggtga gcttcagcgg cgttaccgcc catcaccacg acgaggttgg cgttcttgat
     5941 gtcgacccag tggttggtca tcgcaccgcg accaaatgtt ggagcaagac ttgctaccgt
     6001 tggtccgtgt cagacacgcg cctggttgtc gaccgcgagc atacccagcg cgcgggagaa
     6061 tttttgcgtt aaatagccgg tttcgttact cgacgcggaa gcacacagca tcccggtgga
     6121 gagccagcgg ttaacagtca cgccttcggc gttttgcgca atgtagttag catcgcggtc
     6181 ttctttcatc agtttggcga tgcgatcaaa cgcctcttcc caactgattt gctgccattt
     6241 atcagaacct ggcgcacggt attccggaaa cttcagacgg ctttcggagt ggatgaaatc
     6301 caccaggcca gcgcctttcg gacaaagtgc accgcggttg accgggtgat ccggatcgcc
     6361 ttcgatatgg aagatagatg ctttggcgtt ttttgctccg tcaccgaggc tgtacatcaa
     6421 cagcccacag cctacggaac aataggtgca ggtattacgg gtttcgcggg tgcgcagcag
     6481 tttatactgc cgggtttccg cgagtgctac gctgggtgca aaacctagtg ccgctgccgt
     6541 ggtgcctgcc ataccgccag cgcagatctt aaagaactgc cttctgctga cctgcatgga
     6601 ttgctccttg tttcgacatt gtcacgtccc atttacattc gcttgctgcg tgtgcaggga
     6661 gtgggagtta tttttctttg cggaaggggc cgcaaaggtc cagaattggc tcgatttccc
     6721 tccatccagg aagggtttgt aacagaatac cataatgttg gtgtgtgtgt tcttatctgg
     6781 ttaagagaaa gtgaaaaaaa cacagcgaaa agaaatcgaa aatgtgacaa atatcacagg
     6841 tgttcgtcaa attgagttat ggcggcgtga tgatttacaa cacccacggt tagatgaggt
     6901 cgcggaagaa gttcccgttg cgctggtcta caacggcatt tcgcatgtgg tgatgatggc
     6961 gtcgcccaaa gaccttgagt actttgcgct cggtttttcg ctttccgagg ggattatcga
     7021 aagtccgcgc gatatcttcg gcatggatgt cgttccttcc tgtaatggtc ttgaagtaca
     7081 aattgagctt tccagccgcc gctttatggg gttgaaggag cgccgccggg cgctggcggg
     7141 acgtacgggc tgtggcgtat gcggcgtgga gcaacttaat gacatcggaa aaccggtgca
     7201 gccgctaccg ttcacccaga cgtttgatct caacaaactg gatgatgcat tacgtcatct
     7261 caacgatttt cagccagtgg ggcaactgac tggttgtact cacgccgctg cctggatgtt
     7321 gccatctggc gaactggtcg gcgggcatga agacgtgggg cgccatgtgg cgctggacaa
     7381 actgttaggc cgccggtcac aagaagggga aagctggcag caaggtgcgg tactggtttc
     7441 cagtcgtgcc agttatgaaa tggtgcaaaa gtcggcgatg tgcggcgtag agattttgtt
     7501 tgcggtgtct gccgcgacca cgcttgctgt agaagtggct gagcgctgta atctgacact
     7561 ggtaggtttt tgtaaaccgg gtcgggcaac ggtttatacc catccgcagc gtttgagcaa
     7621 ttaattaaaa taagtcctgc gatatatatt aaattgcagg acttattcat ttcgtgaatt
     7681 ttattatttt tatttataaa taaacgcgtg tagtggcgtt ttcttttttc atttattatg
     7741 ttcactctct tatctatata tttataaggc aattaaatga aaaggaattt attatcttcc
     7801 gctattatag tcgccatcat ggccctcggt ctgacgggtt gtgatgataa aaaagccgaa
     7861 acagaaacgc ccccgcctgc caatagtcaa cctgccgcac cagctcctga agctcccgtt
     7921 gcaaaagcag aagctaaacc tgaaacacct gcgcaaccgg tggtcgatga acaagcggtt
     7981 ttcgacgaaa aaatggatgt ctatatcaag tgctataaca agttacagat ccctgtacag
     8041 cgcagtctgg cgcgttatgt tgactggctg aaagatttta aacaggggcc taccggtgaa
     8101 gagcgtactg tttatggcat ctacggcatt agtgaatcca acctcgctga gtgtgaaaaa
     8161 ggcgtaaaaa gtgctgtggc gttaacgcct gcgctgcaac caattgatgg cgtggcggtg
     8221 agttatatcg atgctgccgt ggcgttgggt aataccatta acgaaatgga taaatattac
     8281 acccaggaaa attataaaga cgatgcgttt gcgaaaggga aaacgctgca ccagacattc
     8341 ttaaaaaatc tggaagcctt tgaacctgta gcagaatctt atcatgcggc gattcaggaa
     8401 attaacgata agcgccagct tgcggaactg aaaaatattg aagaaagaga agggaaaata
     8461 ttccactact attctctggc agtcatgatt tcagcgaaac aaattaataa cctgatatcg
     8521 caagagaagt ttgatgttga tgcggcaatg aagaaagtgt ctgaactgga aacgctggtg
     8581 gcgcaggcca aagaagcgga taaaggcggc atgaatttct cgtttattaa ttcggcaggc
     8641 cagtatcaac tcgaggctaa aaaatacgtt cgccgcatca gagataaagt cccgtac
//
LOCUS       DAZPEB010000163         8488 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZPEB010000163 DAZPEB010000000
VERSION     DAZPEB010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8488
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.166"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(312..890)
                     /gene="mocA"
                     /locus_tag="ACHURJ_003307"
     CDS             complement(312..890)
                     /gene="mocA"
                     /locus_tag="ACHURJ_003307"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFR1282134.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYVNQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIKRPNITNMRQALLMG
                     AHYSVEVENAEIILDIDTPDDFITVQKRYTKI"
     gene            993..1763
                     /gene="yqeC"
                     /locus_tag="ACHURJ_003308"
     CDS             993..1763
                     /gene="yqeC"
                     /locus_tag="ACHURJ_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFR1282135.1"
                     /translation="MKSIVDPSALFIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRAMPLKAPDEHEPCIPKSSCCVIAVMGGHILGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            1811..3436
                     /gene="yqeB"
                     /locus_tag="ACHURJ_003309"
     CDS             1811..3436
                     /gene="yqeB"
                     /locus_tag="ACHURJ_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFR1282136.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(3477..4409)
                     /gene="arcC"
                     /locus_tag="ACHURJ_003310"
     CDS             complement(3477..4409)
                     /gene="arcC"
                     /locus_tag="ACHURJ_003310"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFR1282137.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNVLREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(4457..5842)
                     /gene="hyuA"
                     /locus_tag="ACHURJ_003311"
     CDS             complement(4457..5842)
                     /gene="hyuA"
                     /locus_tag="ACHURJ_003311"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HFR1282138.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(5895..7106)
                     /locus_tag="ACHURJ_003312"
     CDS             complement(5895..7106)
                     /locus_tag="ACHURJ_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFR1282139.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(7164..8360)
                     /gene="dpaL"
                     /locus_tag="ACHURJ_003313"
     CDS             complement(7164..8360)
                     /gene="dpaL"
                     /locus_tag="ACHURJ_003313"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFR1282140.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(8418..>8488)
                     /locus_tag="ACHURJ_003314"
     CDS             complement(8418..>8488)
                     /locus_tag="ACHURJ_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282141.1"
                     /translation="RKYAKPGALLEQLLKEAQERVK"
BASE COUNT         1999 a         2061 c         2222 g         2206 t
ORIGIN      
        1 ttagcttata cagatatcat tgagtgaccg gtgttgagaa atccggtggt ttagggggat
       61 agagcaacaa tcgcaccact cttgccaggt ttggcgggaa atataaaaaa atgtgaggcg
      121 gctcgaaact taagggcttt ttgtgaatga actcacaggt gctaaatgag aattaaaatc
      181 actcctgaaa attattattg ccagcccata tatcactttt tctcttttct caaaatcagt
      241 gattccttat cattatgata aagagtgctg gagggattaa atatttgaac gcaatcgttt
      301 aactacgttt tttaaatttt agtatacctt ttttgcactg taataaagtc gtccggagta
      361 tcaatatcta aaattatttc tgcattttct acttcgacgg aataatgagc tcccataagg
      421 agtgcctgac gcatattggt gatattgggg cgcttgattg cctgcatcag gcatggtttt
      481 gacactaaaa tcggatggcc ggggatgcca ttatggagtg gcagtattgc gccatcattt
      541 cgtaacgacc agatttttct gaaaatatcg atggtgaggg ttggcatgtc accgtgggtg
      601 agaaaacaat gttctgtttg cactgcgggt actgcggcct ttactgatgt tagtaaaccc
      661 tgcgcataat ctgggttgtg gataatagtg atattgctct ggttcacata acgttcgtgc
      721 agttcattac cacgatagcc ggtgactaaa ataattcggc tacaaaactg caacgcattt
      781 ttgatacttg tatcaagaat tgtcccctgt tgccagggta acatcatttt ccattgcccc
      841 attcttgatg ataatccggc agcggtaatt atacagtcga tggctgacat agtggttcca
      901 ttatttatag gggctaataa cgcctgataa ttgcgttact tcctgatgga ttaagttggg
      961 gataatttat cccagagagg tcataaagac tcgtgaaaag tatagttgac ccatcggcat
     1021 tattcattga tttaggtgcg cagaaacgtc ccactgtgat ttctgttgtt ggtgctgggg
     1081 ggaaaaccag cttgcttttt tggctggcag aactgctcca ggctagcggc aggcgtgtgt
     1141 taatcacgac gacaacacat atgtttatgc caacgtctca ctggcccgtg gttttctgtc
     1201 gtgatcccgc catgcttcct catgcgtctc ttacatcccc catttcattt tgttttcaca
     1261 gctggaaagc gaaccaggga aaagtgcagg gatttacgcc agaagcgatt gatgcactgg
     1321 tgcaacgacc agagtgtgac gtaattctca ttgaagcaga tggctcgcgt gcaatgccgt
     1381 taaaagcgcc tgatgagcac gaaccttgca tacccaaaag cagttgctgc gtgattgctg
     1441 tgatgggagg acatattttg ggcgcgaaag tgagcacaga aaatgtccat cgctggtcgc
     1501 agtttgctga tattactggg ttaacacctg atgcaacctt gcaactgagc gatctcgttg
     1561 cgctggttcg ccaccctcag ggggcgttta aaaacgtacc gcaaggttgt cggcgagtct
     1621 ggttcattaa ccgtttttct caatgtgaga atgcgattgc acaaagcgag ctccttcaac
     1681 cgctgcaaca acacgacgta gaggcaatct ggctaggcga tatacaagaa catcctgcaa
     1741 tcgcgcgcag atttgtgaat tagcggcccg ggtattccgg gttactgaaa ggatatccat
     1801 tgaggttggt atgaatattt tcacagaggc tgcaaaactc gaagagcaaa attgtccgtt
     1861 tgcgatggcg caaattgttg atagccgagg ctcgactccc cgccattctg cacaaatgtt
     1921 agtgcgcgcc gatggttcta tcgtcggtac aattggtggc ggaatggttg agcggaaggt
     1981 gattgaagag tcacttcagg cattgcagga acgtaagccg cgattattcc atggacgtat
     2041 ggctcgtaac ggtgcggatg ctgtcgggtc agattgtgga ggtgcaatgt cagtatttat
     2101 cagcgtccat ggtatgcgtc cacgtctggt gttgattggc gcggggcatg tcaaccgggc
     2161 gatagcccag agtgccgcgc tattaggatt tgatattgcc gttgccgata tttatcgcga
     2221 aagcctcaat cctgaactat tcccaccatc aaccacgctt ctccatgctg agtcgtttgg
     2281 tgcggcagtg gaagcactgg atattcgccc tgataatttt gtcctgattg ccacgaataa
     2341 tcaggatcgt gaagccctcg acaaactcat tgaacagccc attgcatggt tggggttgct
     2401 ggcaagtcgc cgcaaggttc agcttttcct gcgtcaattg cgtgagaaag gcgtggctga
     2461 agaacatatt gcccgtttac atgcgcccgt tggttacaac ataggtgcgg aaacgccgca
     2521 ggagatcgcc atcagcgtgc tggcagaaat attacaggtg aaaaataacg cgccgggtgg
     2581 gctgatgatg aaaccttctc atccttccgg acaccagctg gtggtgattc gcggtgcggg
     2641 agatatcgcc agtggtgtgg cgctacgtct gtatcatgcg ggttttaaag tgatcatgct
     2701 ggaagtggaa aaaccgacag tgattcgttg taccgtggcg tttgcccagg ccgtgttcga
     2761 tggcgaaatg acggtcgaag gcgtcactgc tcgcctggca accagctctg cggaagcgat
     2821 gaaacttacc gaacgcggat tcatccctgt gatggtagat cccgcctgtt cattgcttga
     2881 tgaactgaaa ccgctttgcg tggtggacgc tattctggcg aaacagaatt tgggaacgcg
     2941 ggcagatatg gcaccagtaa caatcgcgct tgggccgggc tttactgcag ggaaggattg
     3001 tcatgcggta attgaaacaa atcgcgggca ctggctcggt caggtgattt actctggttg
     3061 tgcgcaggag aataccggtg ttcctggcaa tattatgggg cataccaccc gacgggtgat
     3121 ccgtgctcct gctgcaggca ttatgcgatc caacgtgaaa ttaggcgatc tggtgaaaga
     3181 gggcgatgtg attgcctgga ttggtgagca tgaaattaaa gcaccgttga cggggatggt
     3241 gcgtggcttg ttgaacgacg gcctggcagt ggttggtggt tttaaaattg gtgatatcga
     3301 tcctcgtggt gaaaccgctg atttcaccag cgtttctgat aaagcccggg cgattggcgg
     3361 cggcgtactt gaggcgttaa tgatgttgat gcatcagggc gtgaaagcga caaaagaagt
     3421 gctggaagtg gcttaaatga aaatggccgg atggtattac ccacccggcc ttaaaattac
     3481 tgacaaatca ccgttccggt tttgccttca atcccttctt tcgctttgct caatacggta
     3541 atcagtgctt cacgacctgc gcgagagcga gcaaacgagg cggcagcttc cactttaggc
     3601 agcatagaac ctttcgcaaa atgaccttct tcgataaagc gttcagcatc acttaatgac
     3661 aggcgatcga gccactgttc attctcttta ccgaagttaa tggccacttt ttctaccgca
     3721 gtcagaatga tcagcatatc ggcatcaatc atttctgcta aacgggcgct ggcccagtct
     3781 ttatcgataa ccgcgctggc accacgcaga tggttacctt cacgaataac agggatacca
     3841 ccaccgccaa cggtgatcac cacctggcct gcatctacca gagctttaac agtttctttt
     3901 tcaatgatat caaccggttt tggcgaggca acaacacggc gatagccgcg accagcatct
     3961 tctttcagag tgtaaccctg ttttgtcagt tgctctgctt cttgctcggt aaagaacgaa
     4021 ccgatcggct tggtcgggtt gaggaatgcc ggatcgttag cgtcgacttc gacctgagta
     4081 accagcgttg ctacaggttt attaatgccc cgagaaagca gttcttctct taacacgttt
     4141 tgtagatcgt aaccaatata accctggctt aaggcaacac agacagacat cggcagcatt
     4201 ggcgagtgtg cttcagtttt tgcggcagct tcaaaggcct ggttaatcat gcccacctgc
     4261 ggcccgttac catgagtgac gataacttca tgtccttggg caattaaatc aacaattgcc
     4321 tgagaagtaa tttttaccgc tttcatttgt ccggccaggt catcgcccag cgcattcccg
     4381 cccagggcga gaacaatttt cttactcatt tttcctcact catcattctt gattgtgtat
     4441 agatccctga cagtggttag agcacgggag ggacaaacgg tttgcgtcgc aggaaacgac
     4501 ctcgcccggc tttgcctgta aaggtgccgt cacagaaaat cgtttcacca cgggataagg
     4561 ttctgacaat cgcgccctga caggtaaaac cctcccatgg cgagtagtcg gcgttgtcgt
     4621 ggagatggcg atgctgaatt tgttggctct gacgtgggtc gataatcacc acgtcgccat
     4681 cggaaccggg cgctaataat cctttttgcg gccacaggcc aaacagcctg gcgggcattg
     4741 cgctggttaa ttcaacaaag cgttccggtg ttatacgtcc cgtcatcacg ccactggaaa
     4801 acagtaactg catgcggttt tccacaccgg gtaagccatt tgggcagcga ctgaaatcgc
     4861 ctttagaaat ttgcaggcgt tgagccatcg agaaggtgca gtgatcggtc gccaccacgt
     4921 caatcgcacc atcgctgatg ccacaccaca gtttgtcctg ctcgcgtacg ttacgcagcg
     4981 gtgggctaag aatgaacttc atgccatctt ctgtatcgta actgcgttcg tccaacagga
     5041 gatattgtgg gcaggtttca acccagactg gctggtgatt cgcacgggca agacgcagat
     5101 aatccagacc taagccgtta gacaggtgca cgatatagag cggggcgtta ccggcaattt
     5161 gtgccaggtt aatcatgcgg gcgatggctt ccgcttcgca ttccagaggg cgactcaagg
     5221 catgatagcg cggcgcggtt aaccctgcgg cgataaattc cgcccgcttg ctggcgatag
     5281 ccgcatcatt ttccgggtgc acggtggtca gcgcgccgga ttcatgcaag cggcgtaatg
     5341 cctgcaaaac ctcgtcatcg ttgagtttgt attgataggt taaatagagt ttaaaactgc
     5401 tcagtccttc ctcgaccatc atcgggattt cgtcgaggat tgcgtgatta atgtgctgga
     5461 tcacaccgtg aaagctgtaa tcgatgaccg ctttatgggc ggcataacca cgataaacct
     5521 ccagttgatg gcgtaaccga cagccgtttg ggccaaatcc catatggtca ataatggttg
     5581 ttgtaccgcc acacgcagct gcgcgggtac cggtaaaaaa atcatcacaa ctgcgcgcga
     5641 tgccgacatc aatattgaaa tgcgtatgga catccacgcc gcctgggaaa acgtaacagc
     5701 cagtggcatc aatttcttca tacgggagct gcggcgaaat attgttgccc aactggcgaa
     5761 caatcccgct ttcaatcagc aaatcctgtt tggcttgtcc atctgcgtta acgacagtgc
     5821 cgtttttgat caatacgcgc atagcaaact ccaaaaaccc tccggcgaac cggaggggaa
     5881 taagtgaaga gtaattactc tgttgccagc cagcttaacg ggattgcagc gtacatcgcg
     5941 gcacaggtca ccaggtgaga tttccaggtt ttttcgttag gtgcatgcgc ttcaggttct
     6001 ttacccgggc caaagccgat caccggaatg ccgtgacggc ccatgataga tacgccgtta
     6061 gttgagaagg tccacttatc aacaaccggc gctttgccaa acagcccttc gtaggcattc
     6121 accagtgctt taacggtgaa gtgatcttct tccactttcc aggtcgggaa gtagcattcg
     6181 gttgggtaaa ccaggccagt ccaggacgga cggtcgtagt tgtacataga aacaaccgcg
     6241 ttagctttct gtactgcagg cagggcgcgg atttcgtcca gcgcgccttc ccaggtttcg
     6301 ccccaggtca gacggcggtc aatagagacg gcgcagctat ctgctacagc gcaacggctt
     6361 ggggatgtga agaagatttc agaaacggtg agggtgcctt tgccgaggaa ttcgtcataa
     6421 cccagacgtt gggagagttc ttgtaattcg ccaagaatcg gacccatttt gaaaatggcg
     6481 ttgtcaccgc gttctggcgc agaaccgtgg cagctaacac cctgaacatc aatacgaatt
     6541 tccatacgac cgcgctgacc acggtatatc tggcagtcgg ttggttcggt actgaccaca
     6601 aattccgggc gaatgccgga ttgttcaata atgtactgcc agcacagacc gtcgcagtct
     6661 tcttcctgca cagtaccggt aaccagcagg gtatattcat cttccagacc gaggtcttta
     6721 atgattttac cggcataaac catagatgcc atgccgcctt cctggtcgga agtaccgcga
     6781 ccaccgatta gctcatcagt ttccatgcct tcgtacggat cgaagtccca gtttttgatg
     6841 ttgccaatgc cgacggtatc gatatgagcg tccattgcca ccagacgcgg gccgtggccg
     6901 atataaccga gaacgttgcc catcgggtcg atttcaactt tatcgaagcc gactttttcc
     6961 atctcttctt taatacgatg tactacgcgt ttttcgtcgc agctttcact gggaatagca
     7021 accatgtcgc gcaggaagcg agtcatgtcc gcctggtaat cttttgcttt ttcaagaatc
     7081 agtttgaatg gaatattctt agccattatt tttctccaga ttttttactc cccgttttgc
     7141 agggagtagt tccaataggg tgattaaggt gctacagcgt gtttgccttc ccagacaact
     7201 tcgcggtagt gcttcacgtc ggtgtcgcct tcagtactga taaccagcac tacggcatct
     7261 ttgttcagcg ccagtttttc catcaggctt tgacgttgcg ggtgataatg aaccgctgcg
     7321 agaacgccca aaccgacagc gccggattca ccggagatga tgcgcgggtc gttgccgtac
     7381 ggattaccca gcacgcgcat acctaatgcg gcaacgctgt cctggcagga gatgaattgg
     7441 gtggcgcagt tacgtaggat ttcccagccc agcgggttag gttcgccaca ggccaggccc
     7501 gccatgatgg tggccatatc accgccaacg ttgacgatgt cgcctttgac gccggagcga
     7561 taaatacagt cagctttgtc aggttcaaca ataatgctgt gcagattttg cgggctgtag
     7621 acgtcgacca gataacccag cacaccaccg gccattgctc cgacaccggc ttgcagcaga
     7681 acgtgcgtcg gggttacgcc catttcacgc atttgctcga cggcttcatc tgccagggtt
     7741 gcgtagcctt gcatgatcca ggttgggatt ttggtgtaac cttcccatgc cgtgtcctgt
     7801 accacttccc agccgtgctg ctgcgcgtgt tgcatggtca ggcgaacggt atcgtcatag
     7861 ttcatatccg tgacgatgca ctcggcaccg aggttcagaa tggcgtcaac gcgttcctga
     7921 gcagaacctt tcggcatgta aatcaccgca ttctgtccga gttgctgtgc tgcccacgcc
     7981 acaccgcgcc cgtggttgcc gtcggtggtg gtcgcgaaag tcattttttc gccgatggca
     8041 tttttcaggt gctcaaatga cagcgtttcg atatcaagat gatatttttc acacaataat
     8101 tgagcgatgg cgtacgcacc gccaagcatt ttgaaggcgt tcagaccgaa tcgttttgat
     8161 tcgtctttga cgagaatttt cttcacacca aaaaggtttg cgagatcgtc cagcgcacaa
     8221 agcggtgttg ggcgataacc agctattttc tggtggaact ggcgcgccag tttggcctgg
     8281 ctttgcgaaa agagcggtga tgtttcgccg ttgaaaaatt tgttatcggc gatatcaatc
     8341 ttcaatgaga aaacggacat atcctatcct tttggcctct ggaaatagag ggccgaccag
     8401 ctgatcggcc cgaggggtta tttcacgcgt tcttgcgctt ctttcagcag ttgctcgagc
     8461 agtgcacctg gtttggcgta tttacggg
//
LOCUS       DAZPEB010000164         8459 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZPEB010000164 DAZPEB010000000
VERSION     DAZPEB010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8459
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.167"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..202
                     /gene="csgC"
                     /locus_tag="ACHURJ_003315"
     CDS             <1..202
                     /gene="csgC"
                     /locus_tag="ACHURJ_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFR1282142.1"
                     /translation="SLREGGSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIV
                     VTVSDGQALHLSQQWPPSSEKS"
     gene            323..634
                     /gene="ymdA"
                     /locus_tag="ACHURJ_003316"
     CDS             323..634
                     /gene="ymdA"
                     /locus_tag="ACHURJ_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFR1282143.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQVASVKVQYIDKQKKLAVMNIEY
                     N"
     gene            730..1263
                     /gene="ymdB"
                     /locus_tag="ACHURJ_003317"
     CDS             730..1263
                     /gene="ymdB"
                     /locus_tag="ACHURJ_003317"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFR1282144.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            1205..2686
                     /gene="clsC"
                     /locus_tag="ACHURJ_003318"
     CDS             1205..2686
                     /gene="clsC"
                     /locus_tag="ACHURJ_003318"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFR1282145.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRQMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(2694..3851)
                     /gene="mdoC"
                     /locus_tag="ACHURJ_003319"
     CDS             complement(2694..3851)
                     /gene="mdoC"
                     /locus_tag="ACHURJ_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFR1282146.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMLVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            4245..5780
                     /gene="mdoG"
                     /locus_tag="ACHURJ_003320"
     CDS             4245..5780
                     /gene="mdoG"
                     /locus_tag="ACHURJ_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFR1282147.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGGAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            5773..8316
                     /gene="mdoH"
                     /locus_tag="ACHURJ_003321"
     CDS             5773..8316
                     /gene="mdoH"
                     /locus_tag="ACHURJ_003321"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFR1282148.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGGCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKHWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
BASE COUNT         2135 a         2005 c         2213 g         2106 t
ORIGIN      
        1 gtccctgcgc gaaggcggtt cagggcaaag tcagacgaag caagaaaaga ccctctcatt
       61 gcctgctaat caacctattg ctttgacgaa gttgagttta aatatttccc cggaagatcg
      121 ggtgaaaata gttgttactg tttctgatgg acaggcactt catttatcac aacaatggcc
      181 gccctcttca gaaaagtctt aatttgttga aatgtcgagc ataagatgaa tctggagaga
      241 atggtctgct gcgaatcagc caacctgaaa gtatggataa cacaaccctc aaggatgact
      301 aatcattgag gaaatagaat aaatgttcag acctttttta aactctctta tgctcggcag
      361 tttgtttttt ccttttattg ccatcgcagg aagcaccgcg caagggggcg tgatccattt
      421 ttatggccaa attgtggaac cggcatgtga cgtcagcacc cagtcatcac ccgtagaaat
      481 gaactgccca cagaatggtt ctgttccggg aaaaacctat tccagcaaag cgttaatgag
      541 cggcaatgtc aaaaacgcgc aagtagcctc agtaaaagtg cagtatattg ataaacagaa
      601 aaagctggct gtaatgaaca tcgaatataa ctaagtttct gaacaacgca cgttgctgag
      661 tagaaaaatg cgatttaacc aaaaagcctg ctgtacactt aagaaacaag aaggtgttaa
      721 ggaggcgtta tgaaaacgcg tattcatgtt gtgcagggtg atattaccaa actggccgtt
      781 gatgtgattg tgaatgcggc taatccgtca ttaatgggag gcggcggcgt cgatggggcc
      841 attcatcgcg cagcgggtcc ggccctgctg gatgcctgtt taaaagtcag gcaacagcag
      901 ggcgattgcc ctacggggca tgccgttatt acgcttgcag gcgatcttcc cgctaaagcc
      961 gtagtacaca ccgtcgggcc agtctggcgt ggtggtgaac aaaacgaaga ccagcttttg
     1021 caggatgcct atctcaatag tctgcgactg gtggcggcaa acagctatac gtcagtggca
     1081 tttcctgcaa tcagtactgg ggtttatggt taccctcgtg cggcagcggc tgaaatcgca
     1141 gtaaaaaccg tttcagaatt tattacccgt cacgctttac ccgaacaggt atactttgtc
     1201 tgttatgatg aagaaaacgc ccacctctac gaaagactcc ttacccaaca aggagatgaa
     1261 tgatttgccc cggctggcga gcgcggtgct gccactgtgt tcgcaacatc ccggtcagtg
     1321 tggtcttttc cctctggaga aaagtctgga tgcgtttgcc gcccggtatc gtctggccga
     1381 aatggcagag catacgctgg atgttcagta ttacatctgg caggacgata tgtcgggtcg
     1441 gttactgttt tccgccctgt tagccgcagc aaagcgtggc gttcgcgtcc gtttgttgct
     1501 ggacgacaac aatacgcccg gacttgacga tattttacgc ttgcttgaca gccatccccg
     1561 cattgaagtc cggcttttta atcctttctc gtttcgcttg ctgcgtccgc ttggttatat
     1621 caccgacttt tcccgtctca atcgccgtat gcacaataaa agtttcactg tcgatggcgt
     1681 ggttaccttg gtgggaggac gaaatattgg tgatgcctat tttggggcag gggaagagcc
     1741 acttttttcg gatttagatg ttatggcaat aggacccgtg gtagaggacg ttgccgatga
     1801 tttcgcccgc tactggtatt gcaaatcggt ttcaccctta cagcaggtgc tggatgtccc
     1861 ggaaggtgaa atggcggatc gcatcgagtt acccgcctcc tggcataacg atgccatgac
     1921 gcatcgttac ttacgccaaa tggaatccag tccgtttata aatcatctgg ttgatggaac
     1981 gttgccgctt atctgggcga aaacacgttt attaagtgat gatccggcga aaggggaggg
     2041 caaggcaaaa cggcattcac tgttaccgca gcgcctgttc gatatcatgg gctcacccag
     2101 tgaacgcatc gatattatct cttcctattt tgtaccgaca cgcgcaggtg tggcgcaact
     2161 cttacggatg gtgagaaaag gggtaaagat tgcgatccta accaattctc ttgccgctaa
     2221 cgatgttgct gtcgtccatg ccggatacgc gcgctggcgc aaaaaattgc tccgctatgg
     2281 cgtggaatta tatgaactca agccgacgcg tgaacaaagt agtacgttac acgatcgcgg
     2341 cataaccggt aattccggtg ccagcctgca tgctaaaacc tttagcatcg atggtaaaac
     2401 ggtgtttatc ggctctttca atttcgatcc acgttcgaca ttgctcaata ctgaaatggg
     2461 cttcgtgata gagagcgaaa cgctggcaca gttaattgat aaacgcttta tacagagcca
     2521 gtatgatgcg gcctggcagc tccgtctgga caggtgggga cggatcaact gggttgatcg
     2581 tcatgcaaag aaagagatta ttctcaaaaa agaacccgcc accagtttct ggaagcgggt
     2641 tatggtcaga ctggcgtcga tattgcccgt ggaatggtta ttgtaaatgt ggcttaacgg
     2701 gctggtgctt tatcgttctc acgcttaaca accggtttac cagaaaacaa aaacttcagt
     2761 aacgggatgc gtaaatgaat ttcatacaga attatcgcaa tccctactac gaatatcagg
     2821 ccacagagaa aaccaagcca gttggaggtg atgtgcggtg taatgtatgc gccgaaaaac
     2881 agcgttaacg ggtggtgaac cagatagata aatagcgatg cgttaacaaa gtaagtcacc
     2941 cgcgctgact ggaagttaag caaacggtgg ccaaaggaga agaccacatt caccatccac
     3001 agaccgagga ccatggtgat caccgactcg gtttcgtaca tccaggcatc gccactgcca
     3061 tagcgttggt tgagtaaata agcgacaaat gccaatgctg ctgcaagggt acagccacga
     3121 gacggcgtgg taaacaaggc tttaagatga gggaaaatga aagccagtgc accgaggata
     3181 aagaacggca aataaaatag cgtttgcatg acaataaaat tgaacatgcc attactcaga
     3241 atgggcggat acacaataaa aatcgttctt cttattaccg catagccgat gccgaggcat
     3301 aaaaaaatca ccgatagttt taccatcgag aattttttat tcgttttatc agaattttct
     3361 aaattatttc tgatgcgttt aaatatccat acgcacagcg tcgtcatgac tactaacacc
     3421 agtaaaaacc acaggtgtga tattaattcc caggccaacg tattatattt gtcatacaat
     3481 gacagcccag gccaactttc cgctttccct ttgacgtatt gcagcataat aaattgcggc
     3541 aatgtcagta aggggatggc tgttaacatc gggataccta cacgttcgac acgtactttc
     3601 caccattttt tcaagggata gcgtaaaaaa agcatgtagg aaaagtagcc ggatataacg
     3661 aaaaatacca gcatgcggaa cgagtggatg aagtcattaa aaagggtcag ccacaatgac
     3721 ggttcggcgc tattcacatg ccatgtatgg ctcgaataga ttaaagaaat atgaaaaggg
     3781 atccctaaca acatcagcca ggcgcggatg gagtcgagga aatattcacg ttgcgcgggt
     3841 actggattca tatatggtta actaatctcg gatttttcgt cttatccctg tcgggttatg
     3901 cctttaggct tgttgccata gcgacaccga cctgaccgcg ccagacgcag gcttcaaggt
     3961 ttttatgcat agcataatcg ctaccactaa ccagaatgga agcgtctgta agacggttga
     4021 taaataaaat tgctggcaaa ccctacacga agtcgatgct tctgtcttta ggagaagcac
     4081 ggaaagtgaa aacggttgca atcaggtgct taatccatga gccagcgtgc tgaacgatac
     4141 cgggattctg ttgtcggaat ggctggttat ccattaaaat agatcggatc gatataagca
     4201 cacaaagggg gaagtgctta ctaattatga aacataaact acaaatgatg aaaatgcgtt
     4261 ggttgagtgc tgcagtaatg ttaaccctgt atacatcttc aagctgggct ttcagtattg
     4321 atgatgtcgc aaagcaagct caatccttag ccgggaaagg ctatgaggcg cccaaaagca
     4381 acttgccctc cgttttccgc gatatgaaat acgcggacta tcagcagatc cagtttaatc
     4441 atgacaaagc gtactggaac aatctgaaga ccccattcaa actcgagttc taccatcagg
     4501 gtatgtactt cgataccccg gtcaaaataa atgaagtgac tgccaccgca gtcaaacgaa
     4561 tcaaatacag cccggattat ttcactttcg gcgatgttca gcatgacaaa gacacggtaa
     4621 aagaccttgg ttttgccggt tttaaagtgc tttacccgat caacagcaaa gataaaaacg
     4681 atgaaatcgt cagcatgctc ggggccagct atttccgcgt gattggtgca ggtcaggttt
     4741 atggcctttc tgcccgcggc ctggcaattg ataccgcctt gccatcgggt gaagaatttc
     4801 cacgcttcaa agagttctgg atcgagcgtc caaaaccgac tgataaacgt ttaaccattt
     4861 atgcattgct tgactcgccg cgcgcgacag gtgcttacaa attcgtggtt atgccagggc
     4921 gtgacacggt tgtggatgtg cagtcgaaaa tctatctgcg cgataaagtc ggcaaactgg
     4981 ggggtgcacc gttaaccagt atgttcctgt ttgggccgaa ccaaccgtcg cctgcaaata
     5041 actatcgtcc ggagttgcac gactctaacg gtctctctat ccatgccggt aatggcgaat
     5101 ggatctggcg tccgttgaat aacccgaaac atttagcggt tagcagcttc tcgatggaga
     5161 acccgcaagg cttcggtctg ttgcagcgcg gtcgtgattt ctcccgcttt gaagatctcg
     5221 atgatcgtta cgatcttcgt ccaagcgcat gggtgactcc gaaaggggag tggggtaaag
     5281 gcagcgttga gctggtggaa attccaacca acgatgaaac caacgataac atcgtcgctt
     5341 actggacgcc ggatcagctg ccggagccgg gtaaagagat gaactttaaa tacaccatca
     5401 ccttcagccg tgatgaagac aaactgcatg cgccagataa cgcatgggtg caacaaacgc
     5461 gtcgttcaac gggggatgtg aagcagtcga acctgattcg ccagcctgac ggtactatcg
     5521 cctttgtggt cgattttacc ggcgcagaga tgaaaaaact gccagaggat accccggtca
     5581 cagcgcaaac cagcattggt gataatggtg agatagttga aagcacggtg cgctataacc
     5641 cggttaccaa aggctggcgt ctggtgatgc gtgtgaaagt gaaagatgcc aagaaaacca
     5701 ctgaaatgcg tgctgcgctg gtgaatgccg atcagacgtt gagtgaaacc tggagctacc
     5761 agttacctgc caatgaataa gacaactgag tacattgacg caatgcccat cgccgcaagc
     5821 gagaaagcgg cattgccgaa gactgatatc cgcgccgtac atcaggcgct ggatgccgaa
     5881 caccgcacct gggcgcggga ggatgattcc ccgcaaggct cggtaaaggc gcgtctggaa
     5941 caagcctggc cagattcact tgctgatgga cagttaatta aagacgacga agggcgcgat
     6001 cagctgaagg cgatgccaga agcaaaacgc tcctcgatgt ttcccgaccc gtggcgtacc
     6061 aacccggtag gccgtttctg ggatcgcctg cgtggacgcg atgtcacgcc gcgctatctg
     6121 gctcgtttga ccaaagaaga gcaggagagc gagcaaaagt ggcgtaccgt cggtaccatc
     6181 cgccgttaca ttctgttgat cctgacgctc gcgcaaactg tcgtcgcgac ctggtatatg
     6241 aagaccattc ttccttatca gggttgggcg ctgattaatc ctatggatat ggttggtcag
     6301 gatttgtggg tttcctttat gcagcttctg ccttatatgc tgcaaaccgg tatcctgatc
     6361 ctctttgcgg tactgttctg ttgggtgtcc gccggattct ggacggcgtt aatgggcttc
     6421 ctgcaactgc ttattggtcg cgataaatac agtatatctg cgtcaacagt tggcgatgaa
     6481 ccattaaacc cggagcatcg cacggcgttg atcatgccta tctgtaacga agacgtgaac
     6541 cgtgtttttg ctggcctgcg tgcaacgtgg gaatcagtaa aagccaccgg gaatgccaaa
     6601 cactttgatg tctacattct tagtgacagt tataacccgg atatctgcgt cgcagagcaa
     6661 aaagcctgga tggagcttat cgctgaagtc ggtggcgaag gtcagatttt ctatcgccgc
     6721 cgccgtcgcc gcgtgaagcg taaaagcggt aatatcgatg acttctgccg tcgctggggc
     6781 agccagtaca gctacatggt ggtgctggat gctgactcgg taatgaccgg tggttgtttg
     6841 tgcgggctgg tgcgcctgat ggaagccaac ccgaacgccg ggatcattca gtcgtcgccg
     6901 aaagcgtccg gtatggatac gctgtatgcg cgctgtcagc agttcgcgac ccgcgtgtat
     6961 gggccactgt ttacagccgg tttgcacttc tggcaacttg gcgagtcgca ctactgggga
     7021 cataacgcga ttatccgcgt gaaaccgttt atcgagcact gcgcactggc tccgctgccg
     7081 ggcgaaggtt cctttgccgg ttcaatcctg tcacatgact ttgtagaagc ggcgttgatg
     7141 cgccgtgcag gttggggggt ctggattgct tacgatctcc cgggttctta tgaagaatta
     7201 ccgcctaact tgcttgatga gctaaaacgt gaccgccgct ggtgccacgg taacctgatg
     7261 aacttccgtc tgttcctggt gaagggtatg cacccggttc accgtgcggt gttcctgacg
     7321 ggcgtgatgt cttatctctc cgctccgctg tggtttatgt tcctcgcgct ctctactgca
     7381 ttgcaggtag tacatgcgtt gaccgaaccg caatacttcc tgcaaccacg gcagttgttc
     7441 ccggtgtggc cgcagtggcg tcctgagctg gcgattgcac tttttgcttc gactatggtg
     7501 ctgttgttcc tgccgaagtt attgagcatt ttgcttatct ggtgcaaagg aacgaaagaa
     7561 tacggcggct tctggcgcgt tacattatcg ttgctactgg aagtgctttt ttccgtgctg
     7621 ctggctccgg tacgcatgct gttccatacg gtcttcgttg tcagcgcgtt ccttggctgg
     7681 gaagtggtgt ggaattcacc gcagcgtgat gatgactcca cttcctgggg tgaagcgttc
     7741 aaacgccacg gctcacagct gctgttaggg ttagtgtggg ctgttgggat ggcgtggctg
     7801 gatctgcgtt tcctgttctg gctggcaccg attgtcttct cgttgatcct gtcaccgttt
     7861 gtttcggtga tttccagccg tgccaccgtt ggtctgcgaa ccaaacactg gaaactgttc
     7921 ctgatcccgg aagagtattc accgccgcag gtgctggttg ataccgatcg cttccttgag
     7981 atgaatcgtc aacgctccct tgatgatggt tttatgcacg cggtgtttaa cccgtcattt
     8041 aacgctctgg caaccgcaat ggcgaccgcg cgtcaccgcg ccagtaaggt gctggaaatc
     8101 gcccgtgacc gccacgttga acaggcgctt aacgagacgc cagagaagct gaatcgcgat
     8161 cgtcgcctgg tgctgctaag cgatccggtg acgatggccc gtctgcattt ccgtgtctgg
     8221 aattccccgg agagatattc ttcatgggtg agttattacg aagggataaa gctcaatcca
     8281 ctggcattgc gtaaaccgga tgcggcttcg caataaaaac gtagttgcct gatgcgctac
     8341 gcttatcagg cctacatcgt tcctgcaatt tattgatttt gcaagatttt gtaggttggg
     8401 taaggcgttc acgccgcatc cggcaaaaac aacgagccaa taaaaatacc ggcgttatg
//
LOCUS       DAZPEB010000165         8450 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZPEB010000165 DAZPEB010000000
VERSION     DAZPEB010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8450
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.168"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            323..1300
                     /gene="glaH"
                     /locus_tag="ACHURJ_003322"
     CDS             323..1300
                     /gene="glaH"
                     /locus_tag="ACHURJ_003322"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFR1282149.1"
                     /translation="MNALIAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKPLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            1320..2588
                     /gene="lhgO"
                     /locus_tag="ACHURJ_003323"
     CDS             1320..2588
                     /gene="lhgO"
                     /locus_tag="ACHURJ_003323"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFR1282150.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNDIRYDNCGKMLIATSDLEMERM
                     RALWERTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVVRTRQGGEYEASTLISCSGLMANRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRATRSVDALHAAFNQ"
     gene            2611..4046
                     /gene="gabD"
                     /locus_tag="ACHURJ_003324"
                     /pseudo
     CDS             2611..4046
                     /gene="gabD"
                     /locus_tag="ACHURJ_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            4060..5326
                     /gene="gabT"
                     /locus_tag="ACHURJ_003325"
                     /pseudo
     CDS             4060..5326
                     /gene="gabT"
                     /locus_tag="ACHURJ_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417148.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
     gene            5564..6963
                     /gene="gabP"
                     /locus_tag="ACHURJ_003326"
                     /pseudo
     CDS             5564..6963
                     /gene="gabP"
                     /locus_tag="ACHURJ_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
     gene            6984..7646
                     /gene="csiR"
                     /locus_tag="ACHURJ_003327"
     CDS             6984..7646
                     /gene="csiR"
                     /locus_tag="ACHURJ_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFR1282151.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGSDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7647..8096)
                     /gene="kbp"
                     /locus_tag="ACHURJ_003328"
     CDS             complement(7647..8096)
                     /gene="kbp"
                     /locus_tag="ACHURJ_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFR1282152.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8180..8338)
                     /locus_tag="ACHURJ_003329"
     CDS             complement(8180..8338)
                     /locus_tag="ACHURJ_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFR1282153.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         1907 a         2209 c         2351 g         1983 t
ORIGIN      
        1 ctgccccgat aaaacggggc agataatatg tttagtttac taacggtcat tttgcagtga
       61 agccatttac tgttttttat cgaccagata atctgttctc taatgttaac tccccctaac
      121 ctgttgcttt agttattcat ttcctgtctc actttgcctt aataccctac gttaaatgtt
      181 actaatttgt tgtttttgat cacaataaga aaacaatatg tcgcttttgt gcgcattttt
      241 cagaaatgta gatattttta gattatggct acgaaatgag catcgccatg tcaccctaca
      301 tctcataaga ggatcgcttc tgatgaatgc actgatcgcc gtacaaaata acgctgtcga
      361 ttcaggccag gactatagcg gattcactct catcccgtcg gcgcaatccc cgcgtctgct
      421 ggaactcacc ttcaccgaac agacgaccaa acagtttctc gaacaggttg ccgaatggcc
      481 cgtgcaggcg ctggagtaca aatcgtttct gcgttttcgg gtaggcaaaa ttcttgacga
      541 tctgtgtgcg aatcagctgc aaccattgct gttgaagccc ctgctaaacc gtgctgaagg
      601 tgcgctgttg atcaatgcgg tgggtatcga tgatgtggcg caggcagatg agatggtgaa
      661 gctggcaacg gcggtagcgc atctgattgg tcgctcaaat ttcgatgcga tgagcggtca
      721 gtattacgcg cgtttcgtgg tgaaaaatgt cgataactca gacagctatc tgcgtcagcc
      781 gcaccgcgtg atggaactgc acaacgacgg cacgtatgtc gaagagatca ccgattacgt
      841 gctgatgatg aaaatcgatg agcaaaacat gcagggtgga aattcgttgc tgctgcatct
      901 cgatgactgg gaacatctgg accactattt ccgccatcca ctggcgcgtc gcccgatgcg
      961 ctttgccgcg ccgccgagca aaaacgtcag caaagatgtt ttccatccag tgttcgacgt
     1021 tgatcaacag ggtcgcccgg tgatgcgcta tatcgaccag ttcgtccagc caaaagattt
     1081 tgaagaaggc gtgtggttga gcgagctttc cgatgccatt gaaaccagca aaggcattct
     1141 ttctgtaccc gttcccgttg gcaaattcct gttgataaac aacctgttct ggctgcacgg
     1201 tcgcgaccgc tttactccgc acccggatct gcgccgtgaa ctgatgcgtc agcgtggcta
     1261 tttcgcttac gccactcacc actaccagac gcatcagtaa gcgcaaagga attgagcgga
     1321 tgtatgattt tgtgattatt ggcggcggca tcatcggcat gtcgaccgcc atgcaactga
     1381 ttgatgttta tccggatgcc cgcattgcgt tgctggaaaa agagtccggc ccggcctgtc
     1441 accagacggg ccacaacagc ggcgtgatcc atgccggggt ctattacacg cccggcagcc
     1501 tgaaggcgca gttttgcctg gcgggaaacc gcgccaccaa agccttttgc gatcaaaacg
     1561 acattcgcta tgacaactgc ggcaagatgc tgatcgccac gtccgatctc gaaatggaac
     1621 ggatgcgcgc gttgtgggaa cgcacggcgg cgaacggtat cgagcgcgag tggttaaacg
     1681 ccgaggaact gcgcgagcgc gaaccgaata tcaccgggct cggcggcatt tttgtgccgt
     1741 ccagcggcat tgtcagctat cgcgaagtca cagcggcaat ggcaaaaatc ttccaggcca
     1801 gaggcggtga gattatttat aacgccgaag tcagcgccct cagtgagcat aaaaacggcg
     1861 tggtcgtccg aacccgtcag ggcggcgaat atgaagcatc gacgctgatt agctgttccg
     1921 ggctgatggc taaccggctg gtgaaaatgc tcggcctcga accgggtttt attatctgcc
     1981 cgttccgtgg cgagtatttc cgccttgcgc cggagcataa ccagattgtt aaccatctga
     2041 tttaccccat ccctgacccg gcaatgccgt ttttgggcgt gcatctcacc cgcatgatcg
     2101 acggcagcgt gaccgtcggg ccaaacgcgg tgctggcttt taaacgcgaa ggctatcgca
     2161 agcgcgactt ctcatttagc gacacgctgg aaattttagg ctcgtcgggg attcgccggg
     2221 tgctgcaaaa ccatctacgc tcaggactgg gcgagatgaa aaactcgctg tgcaaaagcg
     2281 gctatctgcg gctggtgcaa aagtattgtc cccggctttc gttaagcgat ctccagccct
     2341 ggcccgccgg tgtgcgggcg caggcggtat cgccggacgg caagctgatt gacgattttc
     2401 tgtttgtcac caccccgcgc acgatccaca cctgcaatgc gccctccccg gcagcgacat
     2461 cagcaattcc tattggtgcg catattgtca gcaaggtaca aacgctgttg gcaagccaga
     2521 gtaaccccgg acgcacgctg cgagcgacac gtagtgtgga tgccttacac gccgcattta
     2581 atcaataacc tttgaaaaca ggacgtaaca atgaaactta acgacagtaa cttattccgc
     2641 cagcaggcgt tgattaacgg ggaatggctg gacgccaaca atggcgggat catcgacgtc
     2701 accaatccgg cgaacggcga caagctgggt agcgtgccga aaatgggcgc tgatgaaacc
     2761 cgcgccgcta tcgacgccgc caaccgcgcc ctgcccgcca ggcgtgcgct caccgccaaa
     2821 gaacgcgcca acattctgcg caactggttc aatttgatga tggagcatca ggacgattta
     2881 gcgcgcctga tgaccctcga acagggcaaa ccactggccg aagcgaaagg cgaaatcagc
     2941 tacgctgcct cttttattga gaggtttgcc gaagaaggca aacgtattta tggcgatacc
     3001 attcccggtc atcaggccga taagcgcctg attgttatca agcagccgat tggcgttacc
     3061 gcagctatca cgccgtggaa cttcccggcg gcgatgatta cccgtaaagc cggtccggcg
     3121 ctggcggcag gctgcaccat ggtgctgaag cccgccagtc agacaccgtt ctctgcgctg
     3181 gcgctggcgg agctggcgat tcgcgcgggc attgcggctg gcgtgtttaa cgtggtcacc
     3241 ggttcggcag gcgcggtcgg taatgaactg accagcaacc cgctggtacg caaactgtcg
     3301 tttaccggtt cgaccgaaat tggccgacag ttaatggaac agtgcgcgaa agacatcaaa
     3361 aaagtgtcgc tggagctggg cggcaacgcg ccgtttatcg tctttgacga tgccgatctc
     3421 gataaagccg tggaaggcgc gctggcctcg aaattccgca acgccgggca aacctgcgtc
     3481 tgcgccaacc gtttggcgtg tatgaccgct ttgccgaaaa attgcagcag gcggtgagca
     3541 aactgcacat cggcgacggg ctggataaag gcgtcaccat cgggccgctg atcgatgaaa
     3601 aagcggtagc aaaagtggaa gagcatattg ccgatgcgct ggagaaaggt gcgcgtgtgg
     3661 tttgcggcgc taaagcggat gaacgcggcg gcaacttctt ccagccgacc attctggtgg
     3721 acgtgccggc caacgccaaa gtgtcgaaag aagagacgtt cggccccctc gccccgctgt
     3781 tccgctttaa agatgaagcc gatgtgattg cgcaagccaa tgacaccgag tttggccttg
     3841 ccgcctattt ctacgcccgt gatttaagcc gcgtcttccg cgtgggcgaa gcgctggagt
     3901 acggcatcgt cggcatcaat accggcatta tttccaatga agtggccccg ttcggcggca
     3961 tcaaagcctc gggtctgggt cgtgaaggtt cgaagtatgg catcgaagat tacttagaaa
     4021 tcaaatatat gtgcatcggt ctttaactgg agaatgcgaa tgaacagcaa taaagagtta
     4081 atgcagcgcc gcagtcaggc gattccccgt ggcgttgggc aaattcaccc gattttcgct
     4141 gaccgcgcgg aaaactgccg ggtgtgggac gttgaaggcc gtgagtatct tgatttcgcg
     4201 ggcgggattg cggtgctcaa taccgggcac ctgcatccga aggtggtggc cgcggtggaa
     4261 gcgcagttga aaaaactgtc gcacacctgc ttccaagtgc tggcttacga gccgtatctg
     4321 gagctgtgcg agattatgaa tcagaaggtg ccgggcgatt tcgccaagaa aacgctgctg
     4381 gttacgaccg gttccgaagc ggtggaaaac gcggtaaaaa tcgcccgcgc cgccaccaaa
     4441 cgtagcggca ccatcgcttt tagcggcgcg tatcacgggc gcacgcatta cacgctggcg
     4501 ctgaccggca aggtgaatcc gtactctgcg ggcatggggc tgatgccggg tcatgtttat
     4561 cgcgcgcttt atccttgccc gctgcacggc ataagcgagg atgacgctat cgccagcatc
     4621 caccggatct tcaaaaatga tgccgcgccg gaagatatcg ccgccatcgt gattgagccg
     4681 gttcagggcg aaggcggttt ctacgccgcg tcgccagcct ttatgcagcg tttacgcgct
     4741 ctgtgtgacg agcacgggat catgctgatt gccgatgaag tgcagagcgg cgcggggcgt
     4801 accggcacgc tgtttgcggt ggagcagatg ggcgtggcgc cggatcttac cacctttgcg
     4861 aaatcgatcg ctggcgggcg tcaccgggcg cgcggaagtg atggacgctg ttgcaccggg
     4921 cggtctgggc ggcacctatg ccggtaaccc gattgcctgc gtggctgcgc tggaagtgtt
     4981 gaaggtgttc gagcaggaaa atctgctgca aaaagccaac gatctggggc agaagctaaa
     5041 agacggattg ttggcgatag ccgaaaaaca cccggagatc ggcgacgtac gcgggctggg
     5101 agcgatgatt gccatcgagc tgtttgaaga tggcgatcac agcaaaccgg acgcaaaact
     5161 caccgccggg atcgtggcac gcgcccgcga taagggcctg attcttctct cctgcggccc
     5221 gtattacaac gtgctgcgca tccttgtact gctcaccatt gaagacgctc agatccgtca
     5281 gggtctggag attatcggtc agtgttttga tgaggcgaag cagtagcacc gtccccatgc
     5341 aggaggcgac gctgcgcgtc ttgtccggcc tacgagaatc caggtcggat aaggcgttta
     5401 cgccgcatcc gacaatctgt acgtgaacag gaataaatct atgtcggccg gataatgcgg
     5461 agccgctctc cggcaaaaag aatcaataac aattaaacgc gtgacccggc gcgggaaatg
     5521 tcggggcgct ctcccaagtg acacactttc gagaggattc aggatggggc aatcatcgca
     5581 accacatgag ttaggcggcg ggctgaaatc acgccacgtc accatgttgt ctattgccgg
     5641 tgttatcggc gcaagtctgt ttgtcggttc cagcgtcgcc atcgccgaag cgggcccggc
     5701 agtattactg gcctatctgt tcgccgggct actggtggtt atgattatgc ggatgttggc
     5761 ggaaatggcg gttgccacgc ccgataccgg ttcgttttcc acctatgccg ataaagccat
     5821 tggtcgctgg gcgggctata ccatcggctg gttgtactgg tggttttggg tactggttat
     5881 cccgctggaa gccaacatcg ccgccatgat cctgcactcg tgggttccag gcattcccat
     5941 ctggttattt tccctcgtca ttacccttgc cttaactggc agtaatttat taagcgttaa
     6001 aaactacggc gaatttgagt tctggctggc gctgtgcaaa gtcatcgcta tcctggcctt
     6061 tattttcctt ggtgcagtcg caattagcgg tttttaccct tatgccgaag tgagcgggat
     6121 ctcaagattg tgggatagcg gcggctttat gcccaacggc ttcggtgcgg tattaagcgc
     6181 gatgttgatc accatgttct cgtttatggg cgcagaaatt gtcaccattg ccgccgcgga
     6241 atccgacacg ccggaaaaac atattgtccg cgccactaac tcggttatct ggcgtatttc
     6301 tatcttctat ttgtgctcta tttttgtcgt cgtggcatta attccgtgga atatgcccgg
     6361 actaaaagcc gtcggttctt atcgctcggt actggaattg ctcaatattc cccatgcgaa
     6421 attaatcatg gactgtgtga tattactttc cgtaaccagc tgcctgaact cggcgctgta
     6481 taccgcttcc aggatgctct actccttaag ccgtcgcgga gatgcgcctg cggtaatggg
     6541 taaaatcaac cgcagtaaaa ccccgtacgt ggcggtgtta ctctccaccg gcgcggcatt
     6601 cttaacggtg gtggtgaact attacgtgcc tgcgaaggta tttaaatttc tgatcgacag
     6661 ctccggcgct atcgccctgc tggtttattt agtcatcgcc gtttcacagt tgcggatgcg
     6721 caaaattctg cgagcagaag gaagcgaaat tcgcttgcgc atgtggcttt atccctggct
     6781 cacctggctg gtcatcggct ttattacctt tgtgttggta gtgatgctat tccgcccggc
     6841 gcaacagtta gaagtgatct ccaccgactt attagcaata gggattatct gtaccgtgcc
     6901 aattatggcg gctggaataa gctgataatg tggcaaaaaa cacccgttca taatacgcgc
     6961 tgataataat caggagtcac accatgacca ttacgtctct ggatggctat cgctggctga
     7021 agaacgatat tattcgcggt aattttcaac cggatgaaaa attacggatg agtttgctga
     7081 catcgcgtta tgcacttggc gttgggccgt tgcgggaagc actttctcaa ctggtggcgg
     7141 aacggctggt cacggtggtg aatcaaaaag ggtatcgggt ggcgtctatg tcagagcagg
     7201 agctgctcga tattttcgat gcccgcgcca atatggaagc gatgttagtg agtctggcga
     7261 ttgcccgcgg tagcgatgag tgggaggcag acgttctcgc aaaagcgcat ctgctcagta
     7321 agctggaagc ctgtgacgcc agcgagaaaa tgcttgatga gtgggatctg cgtcatcagg
     7381 cgtttcatac agcaattgtg gcgggctgtg gttctcacta tttgctgcaa atgcgtgaac
     7441 ggttgtttga tctggcggcg cgctatcgat ttatctggct gcggcgaacg gtgctttcgg
     7501 tagaaatgct ggaggataaa cacgatcagc accagaccct gactgcggcg gtactggcgc
     7561 gagataccgc gcgcgccagt gagttaatgc gccagcattt actgacgcca attcccatta
     7621 tccagcaggc gatggctggc aattaactac tcttccggaa tacgcaacac ttgccccgga
     7681 taaattttat ccgggctttt tagcatcggt ttattcgctt cgaagatttt attgtacaga
     7741 ttagcgttac cgtagacctg tttggaaatg gcactcagag tgtcgccaga cttaacggta
     7801 taaaactggc tggcagtggc tggtgtcgcc gttttcacct gatcatcaac actggcaata
     7861 ccggaaatat tccccaccgc aacaaggatt ttctccttcg cctcctgact caggccgtca
     7921 ccagtgaccg tcgctttgcc gtcggcaatt tgaatgttca ctttatcggc atcaggtata
     7981 ccggttttgc tcagatgctc ctgcaccttc ttcgcctgat cgtctttatc gtgctgacct
     8041 gtaaccgcgt cccagagttt ttctccggca tctttcacaa aattgaacag acccatagct
     8101 acctcattcg ttaacggaaa cactcagaaa gtgtagcaga ggaacgcggc gcgttaacgc
     8161 cgcggggggg agcacaggtt taatcgcggg tttgcaccca gaacgcgtga atcaaaccgg
     8221 ggatataccc aagcagtgtc agcagaatat taataatgaa cgcccaaccg aaccctttac
     8281 cgagcagcac gccgagcggc ggcagaatga tggtgatgac gattctccag aaacccatat
     8341 gtactcccta taagaaaatt actcattgtt taaaaagaga ttttatctct taagcgtagt
     8401 aattttagcg gagcctgcca gtttcagctt acgcttttac tctcatttac
//
LOCUS       DAZPEB010000166         8424 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZPEB010000166 DAZPEB010000000
VERSION     DAZPEB010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8424
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.169"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            45..959
                     /locus_tag="ACHURJ_003330"
     CDS             45..959
                     /locus_tag="ACHURJ_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1282154.1"
                     /translation="MLEMFEKIEKKYWQDKIKKISKFRVIKMQDLDDAKVIEVLAKVL
                     TKKGISKVKHNSYISKVLQITTSAGYKKLNGATKWEVQQLIKVVQSVGMDMIEFFRIY
                     YEDNKEIQDAIWNDGRSEHSCKVCLYPEGSEVNTEYSALKVADQWNIFPTNDLKDELL
                     YESKRSIESIVINPKVISSTKYRIALLDDDANITESLKEILSNDYYQVDTYDNLKKLT
                     QAVATNPYDAYVLDWIIGDETSFKLVKAIRSSKNKNAMIIVLTGQLSGIKDDEIVKSI
                     HDYDIVGPYEKPIKSGIIKSNIEKYFAR"
     gene            963..1946
                     /locus_tag="ACHURJ_003331"
     CDS             963..1946
                     /locus_tag="ACHURJ_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282155.1"
                     /translation="MRNLLEYSGSLISNDSLIVAPYDCQKLSYENILPLNNSLIKNWN
                     IQKFSSDYSEIEHLNVINGMGVTLGDSIVGISVLSAIKKKNPKIKIKLIRPETAPSYV
                     EELYELASSVIDELHYMPFHIQKLPCTALNIDVGNQLYSPDFHIMEMHDYFIQGLGVK
                     LDDVNHNELSNKWLGNIDLGAPVFENYTLFAPIASTKIRSIPSKFYYDIVDMLSRREG
                     KTVLGFVDVNHKNYINISKYSPHTKDFIALIKYASNVYTCDSSALHIAAGFGVPTECV
                     FNTIPPELRTKYYSKCKSIYVGNKRLEKIQSSEDFQLVKMVENNYREYLYG"
     gene            1939..2427
                     /locus_tag="ACHURJ_003332"
     CDS             1939..2427
                     /locus_tag="ACHURJ_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HFR1282156.1"
                     /translation="MVKIISFLSLMLLSISAYSNIGDIVIDGSIANANSDKGYVITEK
                     QFDSFPRDTIVTTTPWTPTGKKVKFKGVDLSYVLKLAGANGAKLKFHALNDYEIIVDM
                     NDVEKYNILLATEMDGKRLQIRDFGPYFLVYPLDEHYIELNTPHYLARFIWQIDKITV
                     LK"
     gene            2424..4142
                     /locus_tag="ACHURJ_003333"
     CDS             2424..4142
                     /locus_tag="ACHURJ_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HFR1282157.1"
                     /translation="MKKNFFIILVALLLLIAFTVSSVVKYTSLLSEKNLYTVAGTQEN
                     YGFSVAKFIIQLNELYTLINSDNTIDDVRLKFDILYSRLNVIYVKSEATAPLYKQQGY
                     EETIDSINKKLEDIDGLLSHNHPDYKKISTIIADIKPLTKTVTNLADMAEIAQRNDAL
                     KDFRSKRQQLWTLLFITGGLISLLLFVLFIYISKINRLLLSERAAFASKNAFLGMVGH
                     ELRTSLQAIVSIIDVVTNNLSGGIKSGQIERLETAVSKMERQLNDLAEFAKIDNGSVE
                     IKNTYNSLQAIVTNAVQDCIAIYEKKDVTVKIKNNNDAVIFTDALRLNQVIENLTSNA
                     IKYTERGEVNVDYFIEKGKVLNIVISDTGKGIPKDKLKFIFKPFTRVVDSKSTVPGFG
                     MGLAIVAGIIRLLKGTIHISSEVNVGTTVTVRIPIKLGDSSLATAEVSASAPGDVFGV
                     LSLLVIDDNEMACSSLSSLLTNAGYIVEATTSPERALEKLLRKPYDVVLSDLQMPGMT
                     GDELYATVSSSQNPNRSTPFIFISAYSDAHSIGQAPLLTKPVRLKDINLKIHEVMSNQ
                     GRVKSE"
     gene            complement(5001..5358)
                     /locus_tag="ACHURJ_003334"
                     /pseudo
     CDS             complement(5001..5358)
                     /locus_tag="ACHURJ_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            5550..6527
                     /locus_tag="ACHURJ_003335"
     CDS             5550..6527
                     /locus_tag="ACHURJ_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000600827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1282158.1"
                     /translation="MILIPDDEPELSLPAASEEFLPALSGENAPVSPARAYLLSLNSH
                     RSRQTMASFLNIVAVMLGAASLESCSWGSLRRHHVMAVTELLRDTGRATATVNTYLSA
                     LKGVAKEAWMLRLMDVESFQHIRAVRNLRGSRLPSGRALPQGEIRALFAVCEADRSCL
                     GARDAAMLAVILGCGLRRSEVVSLDLRDVVTQDRALRVLGKGNKERLAYVPAGAWQRL
                     QIWIDEIRGETPGPLFTRIRRFGDVTLNRLTDQAVYHILQVRQGQAGITKCSPHDLRR
                     TFATAMLDNGEDLITVKDAMGHASVTTTQQYDRRGEQRLQDARDRLNLI"
     gene            complement(6575..6838)
                     /locus_tag="ACHURJ_003336"
     CDS             complement(6575..6838)
                     /locus_tag="ACHURJ_003336"
                     /inference="COORDINATES: protein motif:HMM:NF044836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282159.1"
                     /translation="MLPEQHAAEARYPIPVEDFHFSIFTAVNNQTVMTSSAEIVLVIE
                     GTATLSHASGESLLLHVGLSAFIPASIGRWLLTTTGKVCRVSC"
     gene            complement(6873..>8424)
                     /locus_tag="ACHURJ_003337"
     CDS             complement(6873..>8424)
                     /locus_tag="ACHURJ_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Tn3-like element TnAs1 family transposase"
                     /protein_id="HFR1282160.1"
                     /translation="DIWVKGSRQFRDFDDYLLPAEKFAALKREQALPLAINPNSDQYL
                     EERLQLLDEQLATVTRLAKDNELPDAILTESGLKITPLDAAVPDRAQALIDQTSQLLP
                     RIKITELLMDVDDWTGFSRHFTHLKDGAEAKDRTLLLSAILGDAINLGLTKMAESSPG
                     LTYAKLSWLQAWHIRDETYSAALAELVNHQYRHAFAAHWGDGTTSSSDGQRFRAGGRG
                     ESTGHVNPKYGSEPGRLFYTHISDQYAPFSTRVVNVGVRDSTYVLDGLLYHESDLRIE
                     EHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGETKLYVPQGVQAYPTLRPLIGGTLNI
                     KHVRAHWDDILRLASSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRIERTLFILD
                     WLQSVELRRRVHAGLNKGEARNSLARAVFFNRLGEIRDRSFEQQRYRASGLNLVTAAI
                     VLWNTVYLERATQGLVEAGKPVDGELLQFLSPLGWEHINLTGDYVWRQSRRLEDGKFR
                     PLRMPGKP"
BASE COUNT         2268 a         1929 c         2132 g         2095 t
ORIGIN      
        1 tttaagaatg attccaattt ttaggacttt ttcagctgtg taaaatgttg gagatgtttg
       61 aaaaaattga aaaaaaatac tggcaggata aaatcaagaa aatctccaaa ttcagagtaa
      121 taaaaatgca agatctcgat gatgcaaaag tgatagaggt actggcgaag gtattgacca
      181 aaaaaggtat ttccaaggta aagcacaata gttacataag taaggtgcta cagatcacta
      241 catcagcggg ctataagaag ttgaatggtg cgaccaagtg ggaggttcag caactcataa
      301 aagtagtcca atccgttggc atggatatga ttgagttttt ccgtatttat tatgaggata
      361 ataaagaaat tcaagatgcg atctggaacg atggccgcag tgaacactct tgtaaggtat
      421 gcctctatcc tgaaggttcg gaagtaaaca cagaatactc agcactgaaa gtagctgacc
      481 aatggaacat ttttccgaca aatgatttaa aagatgaact gctttatgaa tcaaaacgaa
      541 gcatagaaag tattgtcata aatccaaagg taattagcag cactaagtat cgaattgcac
      601 tacttgatga cgatgctaat atcaccgaaa gcttaaaaga aatcctttcg aacgattatt
      661 atcaagtaga tacttatgat aacttgaaaa aacttactca agctgtcgca acaaatccgt
      721 atgacgcata tgttttggac tggatcatcg gtgatgaaac aagctttaag ttggttaaag
      781 ctataaggtc gtcaaaaaat aaaaatgcaa tgataatcgt tcttacaggt caactatctg
      841 gtataaaaga tgatgaaatt gtaaagtcaa ttcatgatta tgacattgtg ggaccatacg
      901 aaaagccgat taaatcaggc atcatcaaat ctaacattga aaaatatttc gccagataga
      961 ttatgagaaa tttacttgaa tattccggta gcttgataag taacgatagt ttgatcgtag
     1021 caccttacga ttgtcagaag ctaagctatg aaaatatttt accattaaac aatagtctta
     1081 taaaaaactg gaatatacaa aaattctcaa gtgattactc tgaaatagaa cacctaaatg
     1141 tgatcaatgg catgggagtg acattaggtg attctatcgt cgggatttct gtgctgtcag
     1201 caatcaaaaa aaagaatccc aagattaaaa ttaagcttat tcgaccggag actgcaccgt
     1261 catatgttga ggaactgtat gaattagcat cctcggttat agatgagctt cattacatgc
     1321 cttttcatat tcagaaattg ccttgtactg cattgaatat tgatgtggga aatcagctat
     1381 attctccaga tttccatatt atggaaatgc atgattattt tatccaaggt cttggcgtga
     1441 aacttgatga tgttaaccat aatgagctat ctaataaatg gttaggcaat attgatctcg
     1501 gagcgccagt ttttgaaaac tacacacttt ttgcacctat tgctagcaca aaaataagaa
     1561 gtataccgtc gaagttctat tatgacatag ttgatatgct ttctaggcgc gaaggaaaaa
     1621 cggtactagg tttcgtggat gtgaatcata aaaattatat taacatatca aagtattcac
     1681 cccatacgaa agatttcata gcgctaatta agtatgctag caacgtttat acttgtgact
     1741 cctctgcatt gcatatagcc gctggatttg gtgtacctac tgaatgcgtg tttaatacaa
     1801 taccgccaga attaagaaca aagtattaca gtaagtgtaa gtcgatttat gttgggaata
     1861 aaagattaga aaaaattcag agtagtgaag atttccaact ggttaaaatg gtggaaaata
     1921 attacaggga gtatctttat ggttaaaata atatcattct tatccctaat gcttttgtca
     1981 attagtgcat atagcaatat tggggatata gttatagatg gttcaatcgc taatgctaac
     2041 agtgataaag gttatgtgat tactgaaaag cagtttgatt cgttcccacg ggataccatc
     2101 gtaacgacaa caccttggac gccaaccggt aaaaaagtaa aattcaaggg cgtcgaccta
     2161 agttatgttt taaaactcgc tggcgctaat ggagcaaagc taaaattcca tgctctgaat
     2221 gattatgaaa tcattgtaga catgaatgac gttgaaaaat ataacatttt attggctaca
     2281 gaaatggatg gaaaaaggct tcaaataagg gattttggac cttatttttt ggtatatcct
     2341 ttagatgaac attatattga gcttaacacg ccacactatc ttgcacggtt catttggcag
     2401 atagacaaaa ttacggtgct caaatgaaaa aaaatttctt tatcattttg gttgctctgc
     2461 ttttgttgat agcattcact gtgtctagtg ttgttaagta caccagtctt ttatcggaga
     2521 aaaatttata caccgtcgct ggcacccaag aaaactacgg tttctcagtg gcaaaattca
     2581 ttatacagtt gaatgagctt tacacgctaa tcaatagcga taatactatt gacgatgtta
     2641 ggcttaaatt cgatatcctt tattctcgtt tgaatgttat ttatgtaaag agtgaggcga
     2701 ctgcacctct atataaacag caaggctatg aagaaacaat tgattcgata aacaaaaagc
     2761 ttgaagacat agacgggctg ctttcgcata atcaccctga ttataaaaaa ataagtacaa
     2821 tcatagctga tataaaaccg ctaactaaaa ccgttacaaa tcttgctgat atggctgaaa
     2881 tagcacaaag gaatgacgct cttaaagatt tcaggagcaa acgtcagcag ttatggactc
     2941 tgctttttat tacaggtggg ttgatttcct tacttctttt tgtcctattt atttacataa
     3001 gtaaaattaa ccgattgtta ctatcagaac gggctgcttt tgccagtaaa aatgcatttt
     3061 taggtatggt aggccatgaa ctcagaacat ctttacaggc aatagtgtct atcattgatg
     3121 tcgtaaccaa taatctgagt ggagggatta aatctggtca gatagaacga cttgaaactg
     3181 ctgtttcgaa aatggagcgc cagttaaatg atttggctga atttgcaaaa attgacaacg
     3241 gatctgtaga gataaaaaac acctataata gcttgcaagc aattgttact aacgctgtgc
     3301 aggattgcat tgctatttat gaaaaaaagg atgtcacagt taaaataaaa aacaataatg
     3361 atgcagtgat ttttacagat gctctacgcc ttaatcaagt aattgaaaac ttaaccagta
     3421 acgctattaa gtacactgag cgcggggaag ttaatgtaga ttactttata gaaaaaggta
     3481 aggtattaaa tattgtcatt tctgataccg gaaagggaat ccctaaagac aaactaaagt
     3541 tcatatttaa accatttaca cgagttgtag attcgaaatc cactgtacct ggatttggga
     3601 tgggacttgc tattgttgca gggataatca gactattaaa aggtactatt catatatcta
     3661 gtgaagtaaa tgtaggtaca actgttacag tgagaatacc tatcaagttg ggagatagca
     3721 gccttgcaac agctgaggta tctgcttcag ctcccggaga tgtcttcggc gttcttagct
     3781 tgcttgttat tgatgataat gaaatggcct gttcgtctct ttccagtctg cttactaatg
     3841 ctggatacat tgtcgaggct acaacctcgc ctgaacgagc cctggaaaag cttctgcgta
     3901 aaccttatga cgttgtcctg agtgatttac aaatgccagg catgacaggt gatgagctgt
     3961 atgcaactgt tagtagcagt cagaatccta atcgatccac gcctttcata ttcatcagcg
     4021 catacagtga tgcacactct atcggacagg ctcctttgtt aactaaacct gtcaggttaa
     4081 aagatataaa ccttaaaata catgaggtta tgtcaaatca aggacgagtc aaaagtgagt
     4141 aggcattgtc gtaatatttg cgattatagc tttggtgttc tcgatcgttg cgaattttga
     4201 aaatttgtgg agtcaaatgg cgccgaaagc ccggcgtagc cggttacctg gctataaact
     4261 ctgccaccct accccctgcc ctgattagcc ttaacccgct tacagcaatg aagaagtaaa
     4321 agtaatccct tttaaccccg gtgagcgggc tttacggcgt ttacgacgta aagggcgtga
     4381 tttccggggc cttagcggcg gcagcttgct gctgacgctt agggggatgt tcttcagtta
     4441 tttcagcgac accagggaac ttaacacggt agttgcaaag ctatacccgc taaaaatgtc
     4501 tcagatgcct ctgtacgcgg tcggagtggt cccggcaagg cgtcagccgc agccggggcg
     4561 tatctccgcg ttagcgggcc gaagggccgc ttacgagcgt gtacccgaca actgaccgca
     4621 gctccaccca cagcgatgaa gaagagtagt tacaacaccc atcattaaaa gcgacactac
     4681 tcccggcctt cggccggggg cggcggcgct tttcagtggt ggggaggggc acccggccgg
     4741 caaaccggcg ccggttttat gatgagcttt cttttgaatg ctgtaactaa tttggttctc
     4801 cggtcggggc ccttcgcgaa gcgatgggag ctctttctga tgttgccgaa ccggcgcgac
     4861 cgtcaggtcg ctgcaagagc gggcattctc cggagcctgt ccacagggta ggcgatgaag
     4921 aggttatgtg tttacaggat ttattaaagg atctttaaaa gagatacaga actggtgttt
     4981 aaaaggaact acgcgccctg aaagaccgac ggcaggctca gaagatgctc cagcgtggcc
     5041 atagtacgtt caccgaagac gtgtgcaaca accgtcctcc gtatcctgtc atacgcgtaa
     5101 aacaaccagc gctggcgtga tttagcgccg acgtaacccc actgttcgtc catttccgcg
     5161 caaacaatga cgtcactgcc cggttgtatg cgtgagttta ccgactgcgg cctgagaggc
     5221 ggtgtaagtg aactgtagct gccatgtttt acggcagtga gagcagagat agcgctgatg
     5281 tccggcagta cttttaccgt tacgcaccac gccttcagta gctgagcagg agggacaact
     5341 gatggagatg gaagccacgg gagcacctca aaaccaccat catacactaa atcagtaagt
     5401 tggcagcatc accgtaatcg tacagctaag aacaagtgct tcaagtctac ttattaaaat
     5461 gtacctttga aaaagtagac tttctgcggt acgctctgac agtaagtttg cccccgattt
     5521 tttacgcagc gcgccggacg gagcccgaca tgatcctgat tcccgatgac gaaccggagc
     5581 tgagtctgcc cgcagccagt gaggaattcc tgccggcgct gtcaggtgag aatgccccgg
     5641 tcagcccggc ccgggcctac ctgctttcac tcaattccca ccgcagccgg cagaccatgg
     5701 cctcgttcct gaacatcgtc gccgttatgc tcggagctgc ctccctggag tcctgcagct
     5761 ggggcagcct gcggcgccat cacgtgatgg ccgtgaccga actgctgcgc gacaccggcc
     5821 gggccaccgc caccgtcaac acctatcttt cggcactcaa gggcgtggcg aaggaagcct
     5881 ggatgctccg gctcatggat gtggagagct tccagcatat ccgggcggtc cgtaacctgc
     5941 gcggcagtcg gctgcccagc ggtcgggcgc tgccccaggg ggagatccgc gccctgtttg
     6001 ccgtctgtga agccgatcgc agctgcctcg gggcgcggga tgcagcgatg ctggcggtca
     6061 ttctcggctg cggcctgcga cgatccgaag tagtgagcct ggatttgcgt gacgttgtca
     6121 ctcaggaccg tgcgcttaga gtgctgggta agggaaacaa ggagcgactg gcatatgtcc
     6181 cggccggtgc ctggcagcgg ctgcagatct ggatcgatga gatccggggc gagaccccgg
     6241 gcccgctgtt cacacgcatc cgtcgtttcg gggatgtgac tctaaaccgg ctaaccgacc
     6301 aggcggtgta ccatatcctg caggtgcgcc agggtcaggc cggcatcacg aaatgttctc
     6361 cacacgatct gaggcgaacc ttcgccaccg cgatgctgga taacggggag gatttaatta
     6421 ccgtgaagga tgcgatgggc cacgcgagcg tcaccaccac ccagcagtat gaccgtcgcg
     6481 gggagcaacg cctgcaggac gcgcgggata gactcaacct tatttagggc taagtaaaca
     6541 caaacaaggg ggctgtaccc cctctaattt tacattaaca cgatactcga cagactttcc
     6601 cagtcgtagt cagcaaccat cgcccgatgg aggctgggat aaatgctgac agcccgacgt
     6661 gaagcaaaag gctctcgccg ctggcatggc ttaatgtcgc ggtcccctca atcactaaca
     6721 caatttcagc gctggatgtc attaccgttt gattgttcac cgcagtgaaa atactgaaat
     6781 ggaaatcctc aacaggaata ggatagcgcg cttctgcggc gtgctgttcc ggtaacatag
     6841 gggagcccgc agaattcgga aaaaatcgta cgctaaggtt ttccgggcat ccgtaagggc
     6901 cgaaacttcc cgtcttccag tctgcggctc tgccgccaga cgtaatcgcc ggttaggttg
     6961 atgtgctccc agcccagcgg cgacaggaat tgcagcagct cgccgtccac cggcttgccg
     7021 gcctcgacca acccctgggt ggcgcgttcc aggtacaccg tgttccacag cacgatagcc
     7081 gccgtcacca ggttgaggcc gctggcccgg tagcgctgct gctcgaagct ccgatccctg
     7141 atttccccaa ggcggttgaa gaacaccgcc ctggccagcg agttgcgcgc ctcacctttg
     7201 ttcaggccgg catgcacgcg gcggcgcagt tcaacacttt gcagccagtc caggatgaac
     7261 agcgtgcgct cgatccggcc cagctcgcgc agggccacgg ccagtccgtt ctggcgcggg
     7321 tagctgccga gcttgcgcag catcagcgag gcggtgacgg tgccctgctt gatcgagctg
     7381 gccaggcgca ggatgtcgtc ccagtgggca cgcacgtgct tgatgttcag ggtgccgccg
     7441 atcagcgggc gcaacgtcgg gtaggcttgc acgccctgcg gcacgtacag cttggtttcg
     7501 ccgaggtcgc ggatgcgcgg cgcgaagcgg aagcctagca ggtgcatcag ggcaaagacg
     7561 tgatcggtga agccggccgt gtcggtgtag tgctcctcga tccgcaggtc ggactcgtgg
     7621 tacagcaggc cgtcgagcac ataggtggaa tcgcggacgc cgacattcac cacgcgggtg
     7681 ctgaacggcg cgtactggtc ggagatatgg gtatagaaca gccgtcccgg ctcgctaccg
     7741 tacttcgggt tgacgtgccc ggtgctctcg ccccggccac ccgcgcggaa gcgctggcca
     7801 tcggaggatg aggtcgtgcc gtcgccccag tgggcggcaa aggcgtggcg atactggtgg
     7861 ttgaccagct cggccaaggc cgccgaatag gtttcgtcgc ggatgtgcca ggcttgcagc
     7921 caggacagct tggcgtaggt caggccgggg ctcgactcgg ccatcttggt cagcccgagg
     7981 ttgatcgcat caccgaggat tgcggacagc agcaacgtcc tgtctttggc ctcggccccg
     8041 tccttcaagt gggtgaagtg gcggctgaag cccgtccagt cgtccacgtc catcagcagt
     8101 tcggtgatct tgatgcgcgg cagtaactgg ctggtttggt cgatcagcgc ctgcgcccga
     8161 tccggcaccg ccgcatccag cggggtgatt ttcagccctg actcggtgag gatggcatcg
     8221 ggcagctcgt tgtccttggc caggcgggtg acggtggcca actgctcgtc cagcagctgc
     8281 aaacgctctt ccaggtactg gtcgctgttc gggttgatcg ccaggggcag ggcctgctcg
     8341 cgcttgagtg cggcgaactt ctcggccggc agcaggtagt cgtcgaagtc gcggaactgc
     8401 cgcgagccct tgacccagat gtcg
//
LOCUS       DAZPEB010000167         8413 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZPEB010000167 DAZPEB010000000
VERSION     DAZPEB010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8413
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.170"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(169..375)
                     /locus_tag="ACHURJ_003338"
     CDS             complement(169..375)
                     /locus_tag="ACHURJ_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFR1282161.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMEQAEKATGDFY"
     gene            688..777
                     /gene="kdpF"
                     /locus_tag="ACHURJ_003339"
     CDS             688..777
                     /gene="kdpF"
                     /locus_tag="ACHURJ_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFR1282162.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            777..2450
                     /gene="kdpA"
                     /locus_tag="ACHURJ_003340"
     CDS             777..2450
                     /gene="kdpA"
                     /locus_tag="ACHURJ_003340"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFR1282163.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2473..4521
                     /gene="kdpB"
                     /locus_tag="ACHURJ_003341"
     CDS             2473..4521
                     /gene="kdpB"
                     /locus_tag="ACHURJ_003341"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFR1282164.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADIAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSITNDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4530..5073
                     /gene="kdpC"
                     /locus_tag="ACHURJ_003342"
                     /pseudo
     CDS             4530..5073
                     /gene="kdpC"
                     /locus_tag="ACHURJ_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
     gene            5066..7750
                     /gene="kdpD"
                     /locus_tag="ACHURJ_003343"
     CDS             5066..7750
                     /gene="kdpD"
                     /locus_tag="ACHURJ_003343"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFR1282165.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTDAMLEGLAVLPPKRLAYRGRHICEFDLDAALARR
                     PALILMDELAHSNALGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATIEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDTIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            7747..>8413
                     /gene="kdpE"
                     /locus_tag="ACHURJ_003344"
     CDS             7747..>8413
                     /gene="kdpE"
                     /locus_tag="ACHURJ_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFR1282166.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFHLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYR"
BASE COUNT         1774 a         2187 c         2434 g         2018 t
ORIGIN      
        1 aagtgagata tccatcagat agaaacaatt aggaaaatat ttatatattt ttcctgaata
       61 tatttcagct gggtaatacc atatctaaat atttacctga acatcttcct aataaaaaaa
      121 ccgccagcat ctccactagc ggttttcaat gtattcaaaa ctcggcaatc agtaaaaatc
      181 accggttgcc ttttcggcct gttccatcca aaccggttta tcgctggttt tcgaccagac
      241 gcggtgcagg tagctgtaaa agcgggcgcg gtctttccag aaaagcatga aaggcagcgc
      301 cagaacgccc gctgcaaccg catatgtacg gcgtaaaaag ataagccatg caggatattc
      361 tctgtagagt tccatacttt tctccccctt ttgtatctac ccggtgaata gcaccggaaa
      421 aatgaatttg tttatctgat gaaaatagta ccgccttttg tgtaatttta ctactcatcc
      481 gaccacttat ttttgcttat tgatggttta tttacattca ctctgtaatt aagttgcaca
      541 aaagttaaat taatgctaaa cattagttaa accatggctt ttgccatttt tatacttttt
      601 ttacaccccg cccgcagatt tttgcgaaat ctttgcagcc agaattctac tcttccggta
      661 tcacttttag gccactggag gtgcactgtg agtgcaggcg tgatagccgg cgtgttgctg
      721 gtgtttttat tactgggtta tttggtttat gccctgatca atgcggaggc gttctgatgg
      781 ctgcgcaagg gttcttactg attgccacgt ttttactggt attaatggtg ctggcgcgtc
      841 ctttaggcag cgggctggcg cggctgatta atgacattcc tcttccaggt acagcgggcg
      901 ttgaacgtat actctttcgt ttacctggcg tctctgacca tgagatgaac tggaagcaat
      961 atctttgtgc cattctcggc ctgaacatgc tggggctggc ggtgctgttt tttatgttgc
     1021 tcggtcagca ctatctgccg cttaatccac agcagttgcc ggggctgtcg tgggatctgg
     1081 cgctgaatac cgccgtcagc tttgtcacga ataccaactg gcaatcttat agcggtgaaa
     1141 ccacgttgag ctatttcagc cagatggcag gcttaacggt acaaaacttt ctttctgccg
     1201 ccagcgggat tgcggtgatt tttgccttca tccgtgcgtt tactcgccag agcatgagca
     1261 cactcgggaa tgcctgggtc gatctgctac gcatcacgtt atgggtgcta gtccctgtgg
     1321 cgttgttgat cgcactgttt tttattcaac aaggtgcgct gcaaaacttt ctgccctatc
     1381 aggcagtgaa taccgttgaa ggagcgcaac agctgttacc catgggacca gtagcttctc
     1441 aggaagcgat caagatgctc ggtactaacg gcggtggctt ctttaatgcc aactcgtcgc
     1501 atccgtttga aaacccaacc gcactgacca acttcgtgca gatgctggcg atcttcttga
     1561 tcccaacagc tctgtgcttt gcctttggtg aagtgacggg cgatcgccgc caggggcgca
     1621 tgttgctgtg ggcgatgtca gtgatttttg tcatctgcgt aggcgtggtt atgtgggcag
     1681 aagttcaggg taatcctcat ctgctggcgc tcggcgcaga cagcagcatc aatatggaag
     1741 gtaaagagag ccgtttcggc gtgctggtca gtagcctgtt tgcggtcgtg actacggcgg
     1801 cgtcctgtgg cgcggtgatt gcgatgcatg attcgtttac cgctctcggc ggcatggtgc
     1861 cgatgtggct aatgcaaatt ggtgaagtgg tgttcggcgg tgtcggttct ggtctttacg
     1921 gcatgatgct gtttgtcctg ctggcggtgt ttattgccgg gctgatgatt ggtcgtacac
     1981 cggaatatct gggtaaaaaa atcgacgtac gcgagatgaa actgactgca ctggcgattc
     2041 tggttactcc aacactggta ctgatgggcg cggcgttggc gatgatgacc gacgccggac
     2101 gtagcgccat gcttaaccct ggcccgcatg gttttagcga agtgctgtac gccgtgtcgt
     2161 ccgccgctaa caacaacggc agcgcctttg ccggattaag cgccaactct ccgttctgga
     2221 actgtttact ggcgttctgc atgtttgtcg gtcgcttcgg ggtgattatc ccggtgatgg
     2281 caattgccgg ttcgctggtg agtaaaaaga gccaacccgc cagctccggc acgctgccaa
     2341 cgcacggccc gctgtttgtt ggcctgttaa tcggcaccgt gttgctggtt ggcgcactga
     2401 cctttatccc tgccctggcg cttggtccgg tggcggaata tctctcctga tgatattgag
     2461 tgagcactga atatgagtcg taaacaactg gcgctattcg aaccaacact tgtcgttcag
     2521 gcgctgaaag aagcggtgaa aaaattaaac ccgcaggcgc aatggcgcaa tccggtgatg
     2581 tttatcgtct ggatcggcag tctgctgacc acctgtatta gcatcgcgat ggcaagcggt
     2641 gcgatgcctg gcaatgcgct gtttagcgcg gcaattagcg gctggctgtg ggtcaccgta
     2701 ctgttcgcta atttcgccga ggcgctggca gaaggccgca gtaaagcgca ggccaacagt
     2761 cttaaagggg tgaaaaaaac tgcctttgcc cgcaagctgc gtgagccgaa atatggcgct
     2821 gcggcggaca aagttcctgc cgatcaactg cgtaaaggtg acatcgtgct ggtagaagct
     2881 ggcgatatta tcccctgcga tggtgaagtc attgaaggcg gcgcatcggt cgatgaaagc
     2941 gccatcaccg gggaatcggc accggtgatc cgtgaatccg gcggcgattt tgcctccgtc
     3001 accggcggca cgcgtattct ttctgactgg ctggtgattg agtgtagcgt taaccccggt
     3061 gagacatttc tggatcggat gatcgcgatg gtggaaggcg cacagcgacg caaaacaccg
     3121 aacgagattg ccctgactat cctgctgatt gctctgacca tcgtcttttt actggcaacc
     3181 gccacgctgt ggccgttttc cgcgtggggc ggtaatgccg tgagtgtaac ggtactggtg
     3241 gcgctgctgg tctgtctgat cccaaccact attggcggcc tgttgtcagc gatcggcgtc
     3301 gccgggatga gtcggatgct gggcgcgaat gtgattgcca ccagcggacg tgcagttgaa
     3361 gcggcaggtg acgttgacgt tctgctactg gataaaaccg gcaccatcac actcggtaac
     3421 cgtcaggcgt cggagtttat ccctgcgcag ggcgtggatg aaaaaacgct ggctgacgcc
     3481 gcacaactgg cttcgctggc tgatgaaacg ccggaaggcc gcagtattgt gatcctcgcc
     3541 aagcaacgtt ttaacctgcg cgagcgcgat gtgcagtcgc tccatgccac ctttgtaccg
     3601 tttactgcgc aaagccggat gagcgggatc aacatcgaca accgcatgat ccgtaaaggt
     3661 tctgtcgatg ccattcgccg ccatgttgag gctaacggcg gtcacttccc tgccgatgtt
     3721 gatcaaaaag tcgatcaggt tgcgcgtcag ggggccacgc cgctggtggt ggtggaaggt
     3781 tcccgtgtgc tgggcgttat tgcgctgaaa gatatcgtca aaggcggtat taaagagcgc
     3841 ttcgcccagc tgcgcaaaat gggcattaaa acggtgatga ttaccggcga taaccgtctg
     3901 actgccgccg ccattgctgc ggaagcgggc gtcgatgatt ttctcgccga agcgacaccg
     3961 gaggccaagc tggcattgat tcgtcagtat caggcggaag gccgtttggt ggcgatgacc
     4021 ggcgacggca ccaacgatgc tccggcgctg gcgcaagcag atatcgcggt ggcgatgaac
     4081 tccggcaccc aggcggcgaa agaggcgggc aatatggtcg atctcgactc taacccgacc
     4141 aagttgatcg aggtggtgca tattggcaaa cagatgctga tgacccgtgg ctcgctgacc
     4201 accttcagca ttaccaacga tgtggcgaaa tacttcgcca ttattccggc ggcgttcgcg
     4261 gcaacgtatc cgcagttaaa tgcgctgaac atcatgcgcc tgcattcgcc cgactccgcc
     4321 attctcagtg cagtcatttt caacgccttg attatcgtct ttttgattcc tctggcgtta
     4381 aaaggcgtga gttataaacc gcttaccgct tctgccatgt tgcgccgtaa cttatggatt
     4441 tacggtctgg gtgggctgct ggtgccgttt attggtatca aagtcattga tttactgctg
     4501 accgtttgcg gtctggtgtg aggtttacca tgcgtggctt acgtccggca ttatcgacat
     4561 ttatctttct gttattgatt actggcggcg tttacccgct gctgaccacc gtgctggggc
     4621 aatggtggtt tccctggcag gccaacggtt cgttgattcg tgaaggtgat acggtgcggg
     4681 gttcggcatt aatcgggcag aattttaccg gcaacggcag aaatgcccta taatccacag
     4741 gcttctggcg ggagcaatct ggcggtcagt aaccctgagc tggataaaca aatagccgca
     4801 cgcgttgctg cattacgggc cgctaacccg gatgccagta cgaacgttcc ggttgaactg
     4861 gtgacggcat cggcaagcgg gctggacaat aatatcaccc cgcaagcggc ggcctggcag
     4921 atcccacgca tcgcgaaagc gcgtaatctc agcgttgaac agctcacgca gctgatcgca
     4981 aaatacagcc aacaaccgct ggtgaaatat atcggccagc cggtcgtcaa cattgtcgaa
     5041 ctcaatctgg cgctggataa gcttgatgaa taacgaaccc ttacgtcccg accccgatcg
     5101 tctgctggaa caaactgccg cgccgcatcg ggggaagctg aaagttttct tcggtgcctg
     5161 tgcaggcgtc gggaagacct gggcgatgct ggcagaagcc cagcgactgc gggcgcaagg
     5221 gctggatatt gtggttggcg tggtagaaac ccacgggcga aaagataccg acgcaatgct
     5281 ggaagggctg gctgttctgc cgccaaaacg cctggcgtac cgtgggcggc acatctgcga
     5341 gtttgacctc gatgccgccc tcgcccgccg tccggcgctg atcttaatgg acgaactggc
     5401 gcacagtaat gcgctaggtt ctcgtcaccc taaacgctgg caggacatcg aagagctgct
     5461 ggaagcaggc attgatgttt tcactaccgt caacgttcag catctggaaa gtctgaatga
     5521 tgtggtcagc ggcgtcaccg gaattcaggt acgggaaacc gtgcccgatc cttttttcga
     5581 tgccgccgac gacgtggttc tggtggactt gcccccggac gatctgcgcc agcggctgaa
     5641 agaaggcaaa gtctatattg ccgggcaggc ggagcgcgcc attgaacatt ttttccgcaa
     5701 aggcaatctg atcgccctgc gcgaactggc actgcgccgt actgccgatc gcgttgatga
     5761 gcaaatgcgc gcctggcggg ggcatcctgg cgaagagaaa gtctggcaca cgcgcgacgc
     5821 gatcctttta tgcatcggcc ataacaccgg tagtgagaag ctggtccgcg cggcggcgcg
     5881 gctggcgtca cggctgggta gcgtctggca cgcggtgtat gttgaaaccc ctgccctgca
     5941 ccgcttaccg gaaaaaaaac gtcgggcaat tctcagcgcc ttacgtctgg cgcaggagct
     6001 gggcgcggag acggcaacac tttctgatcc agcggaagaa aaagcagtag tgcgttatgc
     6061 ccgtgaacat aatctcggca agattattct cggtcgcccg gcctcgcgcc gctggtggcg
     6121 tcgggaaacg tttgctgacc gactggcgcg catcgccccc gatctcgatc aggtgctggt
     6181 cgcgcttgat gaaccacccg cccgcacgat taacaacgcg ccggatagcc gctcttttaa
     6241 agacaagtgg cgggtacaga ttcagggatg cgtggttgcc gccgcgttat gcgccgttat
     6301 cactttaatt gccatgcagt ggctgatggc gtttgatgcc gccaacctgg tgatgctgta
     6361 tctgcttggc gtggtggtgg tggcgctatt ttatggacgc tggccttcgg tggttgctac
     6421 cgtcattaat gtggtgagtt tcgatctctt ttttatcgcc ccacgcggca cgctcgccgt
     6481 ctctgatgtg caatatctgc tgaccttcgc agtgatgtta accgtcgggc tggtgatcgg
     6541 gaaccttact gctggcgtgc gttatcaggc gcgggtagcc cgttaccgcg agcaacgcac
     6601 acggcactta tatgaaatgt cgaaagctct ggcggtgggc cgcagtccgc aggacatcgc
     6661 cgccaccatc gaacaattta ttgcctccac gtttcatgcc cgcagtcagg tgttgttgcc
     6721 cgatgacaac ggtaaattgc agccgttaac acatccgcaa ggaatgacgc catgggacga
     6781 taccatcgcg cagtggagtt ttgataaagg cctgcctgcg ggcgcgggca ccgacacgtt
     6841 acccggtgta ccgtaccaga ttttgccgct aaaaagcggc gagaaaacct acgggctggt
     6901 ggtggtggag ccggggaatc tgcgccagtt gatgatcccg gaacagcagc gcctgctgga
     6961 gacgtttacg ctgttagtcg ccaatgccct tgagcggctg acgctaaccg ccagcgaaga
     7021 acaggcgcgg atggcaagcg aacgtgaaca gatccgcaac gccctactgg cagcgctttc
     7081 gcatgattta cgcacgccgc tcacggtgct gttcggtcag gcagaaatct taacgctcga
     7141 tctggcaagc gaaggatcgc cccacgcccg ccaggccagc gagattcgtc agcatgtgct
     7201 gaacactacc cgactggtga ataatctact ggatatggcg cgaattcagt ccggcggctt
     7261 taatttgaag aaagagtggt taacgctgga agaagtggtc ggtagcgcgc tgcaaatgct
     7321 ggaaccgggt ttatcgtcgc ccatcaatct ttctctgcca gaaccgctga ccttaatcca
     7381 cgttgacggg ccactctttg aacgggtgct gattaatctg ctggagaacg cggtgaaata
     7441 tgcgggtgcg caggccgaaa ttggtatcga tgcccacgtt gagggcgaaa atctacaact
     7501 ggatgtctgg gataacggcc ccggtcttcc gccaggccag gagcagacga tatttgataa
     7561 gtttgctcgc gggaataaag agtcggcagt accgggggta gggcttggac tggcaatttg
     7621 tcgggcgata gtggatgtac acgggggcac tattaccgcg ttcaaccgac cggaaggtgg
     7681 tgcctgtttt cgtgttacac ttccccagca aactgcccct gaacttgaag aatttcatga
     7741 ggatatgtga caaacgttct gattgttgaa gatgaacagg ctattcgtcg ctttctgcgc
     7801 acggcgctgg agggcgacgg gatgcgcgtc tatgaggccg aaacgctgca acgcggcttg
     7861 ctggaagcgg ctacccgtaa gccagatttg attattctcg atctcggcct gcccgatggt
     7921 gatgggattg agtttatccg cgacctgcgc cagtggagcg cggtgccggt gattgtgctt
     7981 tccgcacgca gcgaagagag cgacaaaatc gccgcgctgg atgccggagc ggatgattat
     8041 ctgagtaagc cgtttggcat tggcgaattg caggcccgtc tgcgcgtcgc tttacgccgc
     8101 cactccgcta ccgccgcgcc cgatccgctg gtgaaatttt ccgatgttac cgtcgattta
     8161 gccgcccgcg tgattcaccg gggtgaggaa gaggtgcatc tcacaccaat tgagttccac
     8221 ctgctggcgg tgctgctcaa caatgccgga aaagtactca cccagcgcca gctccttaac
     8281 caggtgtggg ggccaaacgc ggtcgaacac agtcactatt tgcgtattta tatgggacat
     8341 ctgcgacaaa aactggaaca ggatcccgcc cgcccacgcc atttcattac tgaaaccggt
     8401 attggctatc ggt
//
LOCUS       DAZPEB010000168         8336 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZPEB010000168 DAZPEB010000000
VERSION     DAZPEB010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8336
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.171"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..1625
                     /gene="mxiD"
                     /locus_tag="ACHURJ_003345"
     CDS             <1..1625
                     /gene="mxiD"
                     /locus_tag="ACHURJ_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HFR1282167.1"
                     /translation="SHLLEQNDIAKYVAQSDTVGSFFERFSALLNYPIVVSKQAAKKR
                     ISGEFDLSNPEEMLEKMTLLVGLIWYKDGNALYIYDSGELISKVILLENISLNYLIQY
                     LKDANLYDHRYPIRGNISDKTFYISGPPALVELVANTATLLDKQVSSIGTDKVNFGVI
                     KLKNTFVSDRTYNMRGEDIVIPGVATVVERLLNNGKALSNRQAKNDPMPPFNITQKVS
                     EDSNDFSFSSVTNSSILEDVSLIAYPETNSILVKGNDQQIQIIRDIITQLDVAKRHIE
                     LSLWIIDIDKSELNNLGVNWQGTASFGDSFGASFNMSSSASISTLDGNKFIASVMALN
                     QKKKANVVSRPVILTQENIPAIFDNNRTFYVSLVGERNSSLEHVTYGTLINVIPRFSS
                     QGQIEMSLTIEDGTGNSQSNYNYNNENTSVLPEVGRTKISTIARVPQGKSLLIGGYTH
                     ETNSNEIVSIPFLSSIPVIGNVFKYKTRNISNIVRVFLIQPREIKESSYYNTAEYKSL
                     ISEREIQKTTQIIPSETTLLEDEKSLVSYLNY"
     gene            1644..2711
                     /gene="mxiC"
                     /locus_tag="ACHURJ_003346"
     CDS             1644..2711
                     /gene="mxiC"
                     /locus_tag="ACHURJ_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HFR1282168.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDQDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVESLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIDVSRASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            2724..4784
                     /gene="mxiA"
                     /locus_tag="ACHURJ_003347"
                     /gene_synonym="virH"
     CDS             2724..4784
                     /gene="mxiA"
                     /locus_tag="ACHURJ_003347"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HFR1282169.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKISANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            4797..5198
                     /gene="spa15"
                     /locus_tag="ACHURJ_003348"
                     /gene_synonym="spaK"
     CDS             4797..5198
                     /gene="spa15"
                     /locus_tag="ACHURJ_003348"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HFR1282170.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNEVL"
     gene            5202..6494
                     /gene="spaL"
                     /locus_tag="ACHURJ_003349"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             5202..6494
                     /gene="spaL"
                     /locus_tag="ACHURJ_003349"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HFR1282171.1"
                     /translation="MSYIKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IIARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGVKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMATAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELLGETIR"
     gene            6575..6925
                     /gene="spa13"
                     /locus_tag="ACHURJ_003350"
                     /gene_synonym="spaM"
     CDS             6575..6925
                     /gene="spa13"
                     /locus_tag="ACHURJ_003350"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HFR1282172.1"
                     /translation="MYRDVEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVK
                     KHIIQYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLI
                     LDELSQEDMKYGIR"
     gene            6912..7790
                     /gene="spa32"
                     /locus_tag="ACHURJ_003351"
     CDS             6912..7790
                     /gene="spa32"
                     /locus_tag="ACHURJ_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HFR1282173.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            7784..>8336
                     /gene="spa33"
                     /locus_tag="ACHURJ_003352"
                     /gene_synonym="spaO"
     CDS             7784..>8336
                     /gene="spa33"
                     /locus_tag="ACHURJ_003352"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HFR1282174.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDLLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQ"
BASE COUNT         2753 a         1176 c         1678 g         2729 t
ORIGIN      
        1 actctcattt attagaacaa aatgacatag cgaaatatgt tgctcaaagt gatacggtag
       61 gatctttttt tgaacgtttt tctgcgttat taaattatcc tattgttgtt agcaaacaag
      121 ctgcaaaaaa gagaatatct ggggagtttg atttatctaa tccagaagaa atgcttgaaa
      181 aaatgacatt acttgtggga ctcatatggt ataaagatgg aaatgcatta tacatatatg
      241 actctggaga attaataagc aaagttattt tgctggagaa tatatcactt aattatctta
      301 ttcagtatct gaaggatgct aacctttatg atcacagata tcctataaga ggaaacatta
      361 gtgataaaac attctatata tctggtcctc cggcattggt tgaactggta gctaatacag
      421 caactttgct tgataagcag gtttcttcta ttgggacaga taaagttaat tttggggtta
      481 taaaattaaa aaatacattt gttagtgaca gaacttataa tatgagaggg gaggatattg
      541 tcatcccagg ggtggctact gttgtagaac gtttattaaa taatggtaag gctctgagta
      601 acagacaagc caaaaatgat ccgatgccac cctttaatat tacacagaaa gtatctgagg
      661 atagtaatga cttttcattt agttcagtta ccaatagttc tatattagag gatgtatcac
      721 ttattgcata tccagaaacc aattctatat tagtcaaagg taatgatcag caaatacaga
      781 ttattcgcga tattatcact cagttagatg tagccaaacg acatatagaa ttatcattat
      841 ggataatcga tattgataaa tctgaattaa ataaccttgg cgttaattgg caaggaacgg
      901 cttcatttgg tgattcgttt ggtgcatcat ttaatatgag ctcatcagca tcgataagca
      961 ctttggacgg caataagttt attgcttcag ttatggcatt aaatcagaaa aagaaagcaa
     1021 atgtagtttc aaggccggtg attttgacac aagagaatat tccggctata tttgataata
     1081 acaggacttt ttatgtgtca ttagttggtg agcgcaattc ttcgttggag catgttacgt
     1141 atggaacatt gataaatgtt atacctcgct tttcatctca gggacaaatt gagatgtctt
     1201 taacgataga ggatggaaca ggaaatagtc agtcaaatta caattataat aatgaaaata
     1261 catctgtcct tccagaagtt ggaaggacca aaatcagtac aatagcgaga gttccacaag
     1321 gcaaaagttt gttgataggt ggttatacgc atgaaacgaa ctctaacgaa atagtatcta
     1381 ttccttttct ctcctctatt cctgtaatcg gtaatgtgtt caagtataaa acaaggaata
     1441 tcagtaatat tgtaagggtt tttctgattc aaccccgtga gataaaggag tcaagttatt
     1501 ataatactgc agaatataag agcctcatat ctgaaagaga aatacaaaag actacacaaa
     1561 taatcccatc tgaaactact ttgctggaag acgaaaaatc attggtttca tacttaaatt
     1621 actaactata aagtaggtga tgtatgcttg atgttaaaaa tacaggagtt tttagctctg
     1681 cattcattga taagttgaat gcaatgacaa attcagatga tggagatgag actgctgatg
     1741 cagagcttga ttctggcttg gctaatagca agtatattga ctcatctgat gagatggctt
     1801 ccgctctttc gtcatttata aacagaagag accttgagaa actgaaagga acaaatagtg
     1861 atagtcagga acgtatttta gatggggaag aagatgaaat taatcacaag atttttgatt
     1921 taaagagaac tttaaaagat aaccttcctc tagatcagga ttttatagac agactaaaga
     1981 gatattttaa agatccaagt gatcaagtct tagcattaag ggaacttttg aatgaaaaag
     2041 atcttactgc tgaacaagtc gaatcattaa ctaaaattat taatgagata atatcaggta
     2101 gtgaaaaaag tgttaatgct ggaataaatt cagctataca ggctaaatta tttggcaaca
     2161 aaatgaaact tgaaccgcag cttttgcgtg catgttatcg tggttttatc atggggaaca
     2221 tatcaacaac agatcagtat atagaatggc ttggtaattt tggttttaat cacagacata
     2281 caattgtgaa ttttgtagag cagtcactga ttgtagacat ggattctgag aaaccgagct
     2341 gtaatgctta tgagtttggt tttgtgttat ctaaattaat tgcaattaag atgattagaa
     2401 cttcagatgt aatttttatg aagaaactgg aatcctcaag cttgctaaaa gatggcagtt
     2461 taagtgcaga gcagctattg ctaactttat tatatatttt tcaatatcca agtgaaagtg
     2521 agcaaattct tacttctgtt atagacgtat cacgagccag tcatgaggat tctgtagtgt
     2581 atcaaacata tctatcttct gttaatgaaa gtcctcatga tatatttaaa agtgaaagtg
     2641 aaagagaaat tgcgatcaat attctacgag agcttgtcac aagtgcatac aagaaagagc
     2701 tttctagata acaggagatg aaagtgatcc agtcttttct taagcaagta agtactaagc
     2761 ctgaattaat tatattagtg cttatggtta tgatcattgc tatgttgatc ataccattac
     2821 ctacatacct tgttgatttt ttaatagggc ttaatattgt acttgctatt ctcgttttta
     2881 tgggatcatt ttatattgaa agaatattga gtttttctac attcccttcg gttttgttga
     2941 taacgacatt atttcgtctc gctttatcaa taagtactag tcgactaatt ttagttgatg
     3001 cggatgcggg taaaattatt actacatttg gccagtttgt cattggtgat agtctggctg
     3061 taggttttgt tattttttca atagtaactg ttgtgcagtt cattgttata acaaaaggat
     3121 ctgaaagggt tgcggaggta gctgctcgct tttctcttga cggtatgccg gggaaacaaa
     3181 tgagcattga tgcggattta aaggctggaa ttattgatgc cgcaggagct aaagaaagac
     3241 gcagtattct ggaacgtgag agtcaattgt atggttcatt tgatggagca atgaagttca
     3301 ttaaaggtga tgcaattgct ggcatcatta ttatttttgt caatttaata ggaggtattt
     3361 ctgttggtat gagtcaacat ggaatgtctc tatctggtgc attatctact tataccatac
     3421 taactattgg tgacggattg gtctctcaga ttcctgctct gttaatttct ataagtgcag
     3481 gatttatcgt tacaagagtt aatggtgata gcgataatat gggacgtaac ataatgtccc
     3541 agatatttgg gaatcctttt gttctcattg ttacatcagc tcttgccttg gcgataggga
     3601 tgttgccagg ttttccgttt tttgtttttt tcctgatagc agttactttg acggctttat
     3661 tctattataa aaaggtcgta gaaaaagaaa aaagtctgtc tgagtctgat tctagtggct
     3721 atactggtac atttgatatt gataatagcc atgactcatc tttggcaatg atagaaaatc
     3781 tggatgctat tagttcagaa accgttcctt taattttatt atttgccgaa aataagataa
     3841 gtgcaaatga tatggaaggt cttattgaaa ggataagaag tcagttcttt attgattatg
     3901 gtgtcaggct tccaactatt ttatatagaa caagcaatga gcttaaggtc gatgatattg
     3961 ttttgttaat aaatgaggtg cgtgccgata gttttaatat atactttgac aaagtgtgta
     4021 ttacagatga aaatggagat atagatgctc taggtattcc tgttgtttca acttcatata
     4081 atgaacgtgt tatttcttgg gtagacgttt catatacaga aaatctaact aatattgatg
     4141 ctaaaattaa aagtgctcaa gatgaatttt atcaccagtt gtcacaagct ttattaaaca
     4201 acataaatga gatttttggt atacaagaaa caaaaaatat gttagatcag tttgaaaatc
     4261 ggtatcctga tctattaaag gaagtcttcc gacatgtgac tatacagaga atttctgagg
     4321 tattacaaag attgcttgga gaaaatattt ctgttcgcaa tttaaaactt attatggagt
     4381 ctttggcgct ttgggctccc agagaaaaag atgtcataac attagttgaa catgtccgtg
     4441 catcactttc taggtatatt tgtagtaaaa tagctgtttc tggtgagatt aaagttgtga
     4501 tgctttccgg atatattgag gatgcaataa gaaaagggat aaggcaaacc tctggtggct
     4561 ctttcttgaa tatggatata gaggtttcgg atgaggtaat ggaaacttta gcacatgctt
     4621 tgagagaatt gagaaatgca aaaaaaaatt tcgttctttt ggtatcagta gatatacgta
     4681 ggtttgttaa aagacttata gataacagat ttaagagtat actcgttata tcgtatgctg
     4741 agattgatga agcatatacc attaatgtat taaagactat ttagtgaggt ttaaatatga
     4801 gtaacattaa tttagttcaa ttagttagag atagtctttt cacgattggt tgtcctccat
     4861 caattattac agatttagac agccattcag cgattaccat ttctttggat agtatgcctg
     4921 ctattaatat agctcttgtc aatgagcagg ttatgttatg ggctaatttc gatgcaccaa
     4981 gtgatgtaaa acttcagtca tctgcttata acatattaaa tttaatgtta atgaacttta
     5041 gttatagcat taatgagctg gtagagcttc atcgttctga tgagtactta cagttaagag
     5101 ttgttattaa agatgactac gttcacgatg ggattgtttt tgcagagatt ctgcatgagt
     5161 tttatcagag gatggaaatc ttaaatgagg tcttataatc aatgagctat ataaaattgc
     5221 tcactcaatt atcttttcct aatagaatct cggggccaat cttggaaaca agtcttagcg
     5281 atgtttcgat tggtgagatt tgtaacattc aggctggaat tgaaagtaat gaaattattg
     5341 caagagctca ggttgtagga tttcatgatg aaaaaacaat attaagcttg attggaaatt
     5401 ctcgtggact ttcacggcaa acgttgatta agcccactgc ccagtttctt catacgcaag
     5461 tcggccgtgg attattggga gcagtagtca atcctttagg ggaggttact gataagtttg
     5521 ctgttacgga taacagtgaa attctttatc gacctgtaga taatgctcct ccgctatata
     5581 gtgaaagggc tgcaattgag aagccttttt taacaggtgt taaggttatt gattctttac
     5641 tcacgtgtgg tgaaggacag cgaatgggga tttttgcgtc agctggttgt ggcaaaactt
     5701 ttctcatgaa tatgctcatt gaacatagtg gtgctgatat atatgttatt gggttaattg
     5761 gtgagcgagg tcgagaggtt acagaaacgg ttgattattt gaaaaactct gagaaaaaaa
     5821 gcaggtgtgt tttagtatat gcaacttcgg attactcttc ggttgatcgt tgtaatgctg
     5881 catatatagc cactgctata gccgaatttt ttaggactga aggacataaa gtagcgcttt
     5941 ttattgattc attaacaagg tatgccagag cattacgtga tgtggcctta gccgctggag
     6001 aatcacctgc cagaagaggc tatccggttt cggtttttga tagcttaccc agacttcttg
     6061 aaaggccagg aaagttaaag gcaggtggct ctattactgc attttacact gttcttttgg
     6121 aggatgatga ttttgctgat ccattagctg aagaggtaag atccatttta gatggacata
     6181 tatatttgag cagaaatcta gcccaaaaag gacaatttcc tgcaattgat tccttaaaaa
     6241 gtataagcag ggtatttaca caggttgttg atgaaaaaca tcgtattatg gccactgcat
     6301 ttcgggagct actttctgaa atagaagagc taaggacaat tattgacttt ggtgaataca
     6361 aaccggggga gaatgcctct caggataaaa tctacaacaa aatatctgtt gttgagagtt
     6421 ttctgaagca agattatcga ctcggtttta cttatgagca gacaatggag cttcttggtg
     6481 aaacaattag ataaggtttt aaaaatcaaa gataaatatc aacggtctgt taaattaatt
     6541 gaagcacaca tattaacact aaaaaaaaaa ctctttgtat cgagatgtgg aggcattaga
     6601 taaaaggatt atatacttcc tgcaactgga gaatgacttg gaacctgtag gcgctcagag
     6661 tgttagtcag cttttcaata caagacgtaa gatcgctata gtaaaaaagc atattatcca
     6721 atatcagtct gaacgaattc ttctaaaggg aagaattgaa gaaattcaaa aagatattga
     6781 tgaggcaaat gcttctaaaa gaaaactgct tcataaagag agtaaaatat gtaaacggat
     6841 tggcttgatt aaaagaaata actttgccaa acagttgata cttgatgagt tatcacaaga
     6901 ggatatgaag tatggcatta gataatataa acctaaattt tagtagtgac aaacagatag
     6961 aaaaatgtga gaaactatct tcaatagata atattgatag tctcgttttg aagaagaaga
     7021 ggaaggttga aattcctgag tactctttaa ttgcatctaa ctattttact attgataagc
     7081 actttgaaca taagcatgat aaaggagaaa tttatagtgg cattaaaaat gcgttcgaac
     7141 ttagaaacga acgagcgaca tattctgata ttccagaatc aatggccatt aaagaaaata
     7201 ttttgatacc agatcaagat atcaaagcaa gggaaaaaat aaatatcggc gatatgaggg
     7261 ggatcttttc atataataag agtggaaatg cagacaagaa cttcgaaaga agtcatactt
     7321 cttctgtaaa ccctgataat ctgctagaat ctgataatag aaatggtcaa attggtttaa
     7381 aaaatcatag cttgtctatt gataagaata ttgctgacat catttcttta ctaaatggaa
     7441 gtgttgctaa atcatttgag ctgcctgtaa tgaataaaaa tactgcagac ataaccccat
     7501 ccatgtcatt gcaagaaaaa tcaatagttg aaaatgataa aaatgttttt caaaaaaata
     7561 gtgaaatgac ttaccacttt aaacagtggg gggctggaca ttctgttagt atttcagtgg
     7621 agtctggttc ttttgttcta aaaccgtcag atcaatttgt aggaaataaa cttgacttaa
     7681 ttttgaaaca agatgctgag ggtaattaca gatttgatag cagtcaacat aataagggga
     7741 ataaaaataa tagtacagga tataatgaac agagtgaaga agaatgctaa gaattaaaca
     7801 ttttgacgct aacgaaaaac tacagatttt atatgcaaag caactctgcg agcgtttttc
     7861 gattcagaca ttcaaaaata aatttacagg cagtgaaagt ttagtcactc ttacttccgt
     7921 gtgtggggat tgggtaattc gtattgatac attatctttt ttgaaaaaaa aatacgaggt
     7981 attttcagga ttttctacac aagaatcttt actgcattta tcaaaatgtg tctttataga
     8041 gtcgtcatct gtattttcga ttccagaact gtctgataag attactttcc ggatcacgaa
     8101 tgaaatccag tatgcaacta ctgggagtca tttatgctgt ttttcctctt ctttaggtat
     8161 tatttatttt gacaagatgc cggtattacg taatcaagtt tctcttgact tattgcatca
     8221 tcttttagag ttttgcttag gttcatctaa tgtaaggctg gctactttaa aaagaattcg
     8281 tactggtgat ataatcatag ttcagaaact ttataattta ttattgtgta atcaag
//
LOCUS       DAZPEB010000169         8316 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZPEB010000169 DAZPEB010000000
VERSION     DAZPEB010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8316
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.172"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(135..211)
                     /locus_tag="ACHURJ_003353"
     tRNA            complement(135..211)
                     /locus_tag="ACHURJ_003353"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(175..177),aa:Asp,seq:gtc)
     gene            complement(344..1075)
                     /gene="dnaQ"
                     /locus_tag="ACHURJ_003354"
     CDS             complement(344..1075)
                     /gene="dnaQ"
                     /locus_tag="ACHURJ_003354"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFR1282175.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            1140..1607
                     /gene="rnhA"
                     /locus_tag="ACHURJ_003355"
     CDS             1140..1607
                     /gene="rnhA"
                     /locus_tag="ACHURJ_003355"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFR1282176.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1604..2326)
                     /locus_tag="ACHURJ_003356"
     CDS             complement(1604..2326)
                     /locus_tag="ACHURJ_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR1282177.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2360..3115
                     /gene="gloB"
                     /locus_tag="ACHURJ_003357"
     CDS             2360..3115
                     /gene="gloB"
                     /locus_tag="ACHURJ_003357"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFR1282178.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            3187..4545
                     /gene="mltD"
                     /locus_tag="ACHURJ_003358"
     CDS             3187..4545
                     /gene="mltD"
                     /locus_tag="ACHURJ_003358"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFR1282179.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRDSLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4594..5217)
                     /locus_tag="ACHURJ_003359"
     CDS             complement(4594..5217)
                     /locus_tag="ACHURJ_003359"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR1282180.1"
                     /translation="MSGLPQGRPTFGAAQNVSAVVAYDLSAHMLDVVAQAAEARQLKN
                     ITTRQGYAASLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSP
                     GHPVRDIWLQAVEALRDTSHVRNYASGAWLTLINEANLIVDNLITDKLPLEFSSWVVR
                     MRTPEALVDAIRIYQQSASTEVRTYFSLQTDGSFTSDIIMVEAHKAA"
     gene            complement(5221..6021)
                     /locus_tag="ACHURJ_003360"
     CDS             complement(5221..6021)
                     /locus_tag="ACHURJ_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFR1282181.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPLGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            6262..7176
                     /gene="yafC"
                     /locus_tag="ACHURJ_003361"
     CDS             6262..7176
                     /gene="yafC"
                     /locus_tag="ACHURJ_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFR1282182.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRAKPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDREIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(7173..7966)
                     /gene="dkgB"
                     /locus_tag="ACHURJ_003362"
                     /pseudo
     CDS             complement(7173..7966)
                     /gene="dkgB"
                     /locus_tag="ACHURJ_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
     gene            complement(8128..8204)
                     /locus_tag="ACHURJ_003363"
     tRNA            complement(8128..8204)
                     /locus_tag="ACHURJ_003363"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8168..8170),aa:Asp,seq:gtc)
BASE COUNT         2218 a         2015 c         2012 g         2071 t
ORIGIN      
        1 tggtctacca tatgtgatta acacatttac agcataacaa aaccatagac cgaagaaata
       61 gcgggattta aatgcaaaaa aaccgcaaaa ttaaaaatct tgcggttctc tgaactcatt
      121 ttcatgagtg aatttggcgg aacggacggg actcgaaccc gcgaccccct gcgtgacagg
      181 caggtattct aaccgactga actaccgctc cgcgttgtgt tccgttggga acgaggcgaa
      241 tattacggat tgcctcaccc ttcgtcaacg gtttttctca tcttttgaat cgtttgctgc
      301 aaaaatcgcc caagccgcta tttttagcgc cttttacagg tatttatgcc cgccagaggc
      361 agcttccgcc cttcttctcc accagatcaa gacgggcttc atgagctgca agctcttcat
      421 ctgtcgcaaa aacaacgcgt aacttacttg cctgacgtac aatgcgctga attgttgctt
      481 caccttgttg ctgctgtgtc tctccttcca tcgcaaaagc catcgacgtt tgaccaccgg
      541 tcatcgccag ataaacttcc gcaaggatct gtgcatcgag taatgccccg tgtagcgttc
      601 gtttactgtt atctatttcg tagcgggcac ataacgcatc gaggctgttg cgcttaccag
      661 gaaacatttt cctcgccacc gcaaggctat cggtgacctt acagaaggta ttggtcttcg
      721 gaatatcgcg cttaagcaac gaaaactcgt agtccataaa gccgatatcg aacgctgcgt
      781 tatggatcac caactccgcg ccgcggatat agtccatgaa ctcatcggct acttcggcaa
      841 acgtgggctt atcgagcaaa aattcatcgg caataccatg tacgccaaag gcttccggat
      901 ccaccagccg atcgggtttg agataaacat ggaagttatt gcccgtcagg cgacggttca
      961 ccacttcaac ggcaccaatc tcaatgatct tgtggccttc atagtgcgca ccaatctggt
     1021 tcataccggt ggtttcggta tcgagaacga tctggcgtgt aattgcagtg ctcatagcgg
     1081 tcatttatgt cagacttatc gttttacagt tcgattcaat tacaggaagt ctaccagaga
     1141 tgcttaaaca ggtagaaatt ttcaccgatg gttcgtgtct gggcaatcca ggacctgggg
     1201 gttacggcgc tattttacgc tatcgcggac gcgagaaaac ctttagcgca ggctacaccc
     1261 gcaccaccaa caaccgtatg gagttgatgg ccgctattgt cgcgctggag gcgttaaaag
     1321 aacattgcga agtcattttg agtaccgaca gccagtatgt ccgccagggt atcacccagt
     1381 ggatccataa ctggaaaaaa cgtggctgga aaaccgcaga caaaaaacca gtaaaaaatg
     1441 tcgatctctg gcaacgtctt gatgctgccc tagggcaaca tcaaatcaaa tgggaatggg
     1501 ttaaaggcca tgccggacac ccggaaaacg aacgctgtga tgaactggct cgtgctgcgg
     1561 caatgaatcc cacactggaa gatacgggct accaagtcga agtttaagct tgtggtttac
     1621 gacattgccg ggtggctcca accgcttggc gaattcgtgg cttgttttta ctctgtttca
     1681 tcgggtttag cgttaaagga atagtccgtt tccgggcaac aataagttgt aagcaaccaa
     1741 gcgcaggaat atgcgcattc aatagttttc ctccgtgttt gttccacggg agaacgtgga
     1801 aacggctggc gtgcagcact tcaaaattca acaaagagag ccagtccagt tgccgcatca
     1861 gagtaaacat ccggctgtta tagggcgagg tttttcgcaa taccggcaca agtttgcgta
     1921 atcccattaa actaatggga ttgaagccac taatcaccag ccagccatca tcaatcaata
     1981 cccgatcggt ttcacgcaat aaacgatgcg gatcggtgca ccacggcaat gtatgtgcca
     2041 gtagacaaac atcaacggat ttatcggcaa aaggaagatg aagtggatcc gcctgcacct
     2101 ggacgggcat tccttgcgca gaaacattca cttgatgaga aaccgcgcac gcttcgcaat
     2161 tgatttctgc gcttaaattg ccaatcttaa gcagatgaaa accatacatt ttagtgaacc
     2221 acgggttgag ctggcgctcc agcgccttgc gataaagctc tccccagggc aaatcgcccc
     2281 agcgatcagg agccacgaca gtttgaggga ctcttgccgg tttcatcaca accttccgtt
     2341 tcacactaag aggtaatcta tgaatcttaa cagtattccc gcctttgatg acaattacat
     2401 ctgggttttg aatgatgaag caggtcgctg cctgattgtc gatcccggag acgcagagcc
     2461 agtattaaac gccattgccg ccaataactg gcaaccggag gccatatttc tcacccacca
     2521 tcatcacgat cacgttggcg gcgtaaaaga actggtggaa aagtttccgc aaattgtggt
     2581 gtatggtcca caagagacac aagataaggg aacaacacag gtagtcaaag atggcgaaac
     2641 tgccttcgtt ttggggcatg aatttagtgt aattgccaca ccgggtcaca ctttaggaca
     2701 tatctgttac ttcagtaaac cttatctatt ttgcggcgac acgctgtttt ctggtgggtg
     2761 tggtaggttg tttgaaggga cagcatcaca aatgtatcaa tcacttaata agttaagtgc
     2821 gttacctgac gatacattgg tatgttgtgc tcatgaatat acgttatcaa atatgaagtt
     2881 tgctttgagt attcttccgc acgatttgtc cataaatgat tattatcgta aagttaagga
     2941 gttacgggca aaaaatcaaa taacactacc cgtaattctg aaaaatgagc ggcaaattaa
     3001 tgttttttta agaacggaag atattgattt aattaatgta attaatgaag aaacattatt
     3061 gcaacaacct gaagagcgtt ttgcatggtt aaggtcaaag aaagataggt tctgataaaa
     3121 ctttcttgtc atcggctccg ttcgccgtta tgatcggtcg tcttttaagc aactattgac
     3181 acacacatga aggcaaaagc gatattactc gcctctgtcc tgctcgtggg ttgccagagt
     3241 accggcaacg ttcaacagca cgcacagagc ctttctgcag ctggtcaagg ggaagcagca
     3301 aagtttacaa gtcaggcacg atggatggac gatgggacgt ctatcgcgcc agatggtgac
     3361 ttgtgggctt tcattggcga cgagctaaag atgggaattc cggaaaatga ccggattcgc
     3421 gaacagaaac agaaatattt acgcaataag agctatctcc acgatgtaac tttacgggca
     3481 gagccgtata tgtactggat agcagggcaa gttaaaaaac gtaacatgcc tatggaactg
     3541 gtactactac ccatagtgga gagcgctttt gatcctcacg caacgtctgg cgccaatgcc
     3601 gcaggcatct ggcagatcat tccgagcacg gggcgcaatt atggtttgaa acagacccgc
     3661 aattatgacg cgcgtcgcga tgttgttgct tcaacaactg ccgcgctgaa catgatgcag
     3721 cgtctgaaca agatgtttga cggcgactgg cttctgaccg tagcggctta taacagcggc
     3781 gaaggtcggg tcatgaaggc aattaaaacg aacaaagcgc gtgggaaatc cacggacttc
     3841 tggtcgttac cgttgccgca ggaaacgaag cagtacgtgc ctaaaatgct ggcattgagt
     3901 gatattctca aaaacagcaa gcgttatggc gtacgtctgc caacgaccga tgaaagccgt
     3961 gctctggcgc gtgtgcacct gagcagcccg gttgaaatgg cgaaggttgc agatatggcg
     4021 gggatttccg tcagcaagct gaagacattc aacgctggcg taaaaggctc cacgctgggc
     4081 gcaagtggcc cgcagtacgt gatggtgcca aagaagcatg cagatcaact gcgtgattct
     4141 ctcgcttcag gcgaaattgc tgctgtacag tcgacgctgg ttgccgacaa tacgccgctt
     4201 aacagccgtg tttacaccgt acgctctggc gacacgcttt caagcatcgc ttcacgtctc
     4261 ggcgtaagca ccaaagattt gcagcagtgg aacaaactgc gcggatctaa gctgaagcca
     4321 ggccaaagtt tgacgattgg tgcaggcagt agcgcacagc ggttggcaaa caacagcgat
     4381 agcattacgt atcgtgtgcg caaaggcgat tcgctttcaa gcattgctaa acgccacggc
     4441 gtgaacatca aagatgtgat gcgctggaac agtgacactg cgaatctgca accaggcgat
     4501 aagctgacgt tgtttgtgaa aaacaacaac atgccagatt cctgacaaac cagataataa
     4561 aaaaggcacc gattcccccg gtgccttttt tatttatgcc gctttatgcg cttctaccat
     4621 gatgatatca ctggtgaaag agccatcagt ctgcaaggaa aaatacgttc tcacctctgt
     4681 cgatgcgctc tgttggtaaa tgcgaatagc gtctactaac gcttctggcg tacgcattct
     4741 cacgacccat gaagaaaatt ccagcggtaa cttatctgta attaaattat caactataag
     4801 attggcttca ttgattaacg tcaaccacgc accgctggcg tagtttcgta cgtgagaggt
     4861 atcgcgtaat gcttctaccg cctgtaacca gatgtcgcgc actgggtgac ccggagacat
     4921 tacatccatc acaatcagcc taccgccagg tttcaatatc ctattcactt ctcgcagtgc
     4981 tgcaccaaca tcatgccagt gatgggcaga ataacggctg ataacaatat caaatgcgtt
     5041 atcggcaaat ggcagacttg cggcatatcc ctggcgggtg gtgatatttt tcagttgccg
     5101 ggcttcggca gcttgtgcca cgacatccag catgtgggcc gataagtcat acgccaccac
     5161 cgcgctcacg ttttgcgcag cgccaaacgt tggtctgccc tgtggcagac ctgacatacc
     5221 ttatttatca ggcttgccgg gactgaattc aacgagtagc ggattgtgat cggaagcgcg
     5281 cgtaaccagt acagaagctt cactgacgtt cagaccacgg tagaaaacaa aatcgagcgg
     5341 acgaccaaac gcccgacggc gctgatcatc ggtaaaacgc acctggcgca gcgacatttc
     5401 ccgcgcaaag cgatataacg cgttcatcct tctacggctc caggcattga aatctcccgc
     5461 cataatgacc gggccgctgt ggtgagctat ctgatcacca ataggaagta actgcttact
     5521 atagacatcc acgcccagac tgaagttgac ggcgtgtata ttaaccacca tcaacaggcg
     5581 ggtgtcaggt aatggataga ccgtcaccag tgccgacttc gccagacgca aaatgggttc
     5641 tcgttcacgt aacgggcagc aatacactgg atgtgccgcc gaaagggtca ttacgccaga
     5701 aggatgttgt ggcagcacga aagcgggtac ctgatcggcg gcaagatagt tagcggtcgc
     5761 aaactgtact aactctggcg ttgtctgcgc ttcctgcaat aacaccaggt gtgcatcttt
     5821 gccgtagttc tttaataccg acaaccattc agcgcgttgc tgtttgtata tgttccacac
     5881 caggatccga atacgctctt cggtacttaa cggttcccca agtggtaatg cctggccgat
     5941 gctcgcaaaa gaccccggcg gtaagatcct ttccgcaggt tgtccggcaa catagcgcat
     6001 ggcataggtg ttttttcgca cttttgactt tgaaacctct attacgtcaa cccttccgca
     6061 ctcgggaggg ttctactatt atagggattt tagctcactc tttcaacgag caattgtata
     6121 ttgttatgta agcaagtgct tacaccggtc agaattttgc tcattatgga gaacattaaa
     6181 tcgcatgaca tatcacgcat cctgatggtt taatgacaaa taaattcaaa atatctcttc
     6241 tattttgcaa caggagcaaa aatgaaagcc acgtcggaag aactcgccat ttttgtttcg
     6301 gtcgtcgaaa gcggcagctt tagccgggca gcggaacaat tagggcaagc aaactcagcg
     6361 gtaagccggg cggtgaaaaa gctggagatg aaacttggcg ttagcctgct taatcggacc
     6421 acgcgacaac ttagcctgac ggaagaaggc gagcgttatt ttcgtcgcgt acagtcaatt
     6481 ttgcaggaga tggcagcggc agaatcagaa attatggaga cgcgtaatac accgcgtgga
     6541 ctgttacgga tcgatgccgc aactccagtg gtgctgcact ttctgatgcc gttaattaag
     6601 cctttccgtg aacgctatcc ggaagtcact ttgtcgctag tctcctccga aacgattatt
     6661 aatttgatcg aaagaaaagt ggatgtcgcg atacgcgctg gtacgttaac ggattccagc
     6721 ttacgtgcca agccgttatt taacagttat cgaaaaatta tcgcctcccc cgattatatt
     6781 tcccgctacg ggaagccaga aacgatcgac gatttaaagc aacatgtttg cctgggattc
     6841 actgaacccg cttccctcaa tacctggccg atagcctgta gtgatggaca attacatgag
     6901 gtgaaatacg gtttgtcatc caatagtggg gaaacactga aacagctttg cctgagtggc
     6961 aacgggattg cgtgtttgtc cgactacatg atcgacagag aaatcgctcg cggagaattg
     7021 gtggagttaa tggcagataa agtgttgcca gtggaaatgc cattcagtgc cgtctattac
     7081 agcgaccgtg cggtaagtac gcgcatccgg gcttttatcg atttccttag cgagcatgta
     7141 aaaacagctc ccggaggagc tgtcagagag gcttaatccc attcaggagc cagaccttcc
     7201 gggctaacca ggcggtcgtt gcaatccagt gcggcgatcg cttttttatc ttcggcatca
     7261 agctgtaaat tttgtgcctt aagattactt tccaggtttt tacgtttagt agaagaagga
     7321 attactgagt aaccttcccc catagcccac gccagaatca cttgtgccgg agtcgcattg
     7381 tgtttagctg cgatacgagc aataacctca tctttcaggg ccttaccata cgccagcgtc
     7441 atataggaag taatatggat gccgtgctgt ttagcccagg caaccacttt acggttttgc
     7501 agataaggag agagttcaat ctggttagta gcgatgtttt cagcgccaac agcagcaatc
     7561 gccttttcca tcaatgggat cgtgaagttg gaaataccga tctcacgcgt cagcccttgt
     7621 tttttggctt ccagcagcgc ctgcataacc ccttcaacag agacttcatc gtttggtgac
     7681 ggccagtgga ttagcgtcag atcaacataa tcggtacgca atttttgcag gctctctttc
     7741 agacttggga tcaatttgtc tttgctgaga ttttcaatcc agattttagt ggtgatgtag
     7801 agttcatgac gtggcacgcc actttctgca atcgcctgac ctactgcggc ttcgttatca
     7861 tagatttgtg cggtatcaat tgcgcgataa ccaagttcaa gcgccgtttt cacagatgaa
     7921 ataacaacgt agtctttcaa cctaaaccaa atgcagggat agccataata tgcctctttt
     7981 aatgctattt tcgttaactg aggtgattat gggcttcaga ctgttgagga aaaaagcgta
     8041 aaatgcagag gatttttgcg attctggcaa taatagatat acagacgaaa aaaaacctcg
     8101 agcgttaact cgaggcttct taataagtgg cggaacggac gggactcgaa cccgcgcccc
     8161 cctgcgtgac aggcaggtat tctaaccgac tgaactaccg ctccaccgaa ttcttttaca
     8221 accaccggtt ttatgaccgg cttactgctt aatttgatgc ctggcagttc cctactctcg
     8281 catggggaga ccccacacta ccatcggcgc tacggc
//
LOCUS       DAZPEB010000170         8313 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZPEB010000170 DAZPEB010000000
VERSION     DAZPEB010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8313
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.173"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(95..1765)
                     /gene="hscC"
                     /locus_tag="ACHURJ_003364"
     CDS             complement(95..1765)
                     /gene="hscC"
                     /locus_tag="ACHURJ_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HFR1282183.1"
                     /translation="MDNAELTIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEIHASAGDSFLGGEDFTHMLVDEVLKRA
                     EVARTTLNESELAALYACVEAAKCSNQSPLHIRWQYQEETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSTQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASDYLAIEIP"
     gene            complement(1849..2784)
                     /gene="rihA"
                     /locus_tag="ACHURJ_003365"
     CDS             complement(1849..2784)
                     /gene="rihA"
                     /locus_tag="ACHURJ_003365"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFR1282184.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHIEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTDNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            complement(2902..3627)
                     /gene="gltL"
                     /locus_tag="ACHURJ_003366"
     CDS             complement(2902..3627)
                     /gene="gltL"
                     /locus_tag="ACHURJ_003366"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFR1282185.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            complement(3627..4301)
                     /gene="gltK"
                     /locus_tag="ACHURJ_003367"
     CDS             complement(3627..4301)
                     /gene="gltK"
                     /locus_tag="ACHURJ_003367"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFR1282186.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNIFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            complement(4301..5041)
                     /gene="gltJ"
                     /locus_tag="ACHURJ_003368"
     CDS             complement(4301..5041)
                     /gene="gltJ"
                     /locus_tag="ACHURJ_003368"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFR1282187.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAIIMLVMTLVERKVRLPGNMGGK"
     gene            complement(5211..6119)
                     /gene="gltI"
                     /locus_tag="ACHURJ_003369"
     CDS             complement(5211..6119)
                     /gene="gltI"
                     /locus_tag="ACHURJ_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFR1282188.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            6574..>8313
                     /locus_tag="ACHURJ_003370"
     CDS             6574..>8313
                     /locus_tag="ACHURJ_003370"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFR1282189.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRDSDQIVISVGASGAIMGIAGAAIATQLASGAGTYHKNQR
                     RVFSLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNMLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVTYQHVADIPLEKYDGAGTSGKPGAWV
                     MALSPDERTLLVPGAGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLR"
BASE COUNT         2074 a         2053 c         2078 g         2108 t
ORIGIN      
        1 atgctctcgg caaaatcgcg atgttgttta gttgacgatg tgttttttgc gccgatgccg
       61 gatgcagcgt ttacgccgca tccggcagcc gtgtttatgg gatctcaatg gctaaataat
      121 cgctcgcccg ccgccgaact tcatccacgc gggccatgtc gtttgactgt agcgccgcat
      181 caaaatcggt gatcacccgg ccaatctctt ctcgctcgtc acccagcgct cgcgcccaca
      241 actcttccag tttggcttta aaggtgcgat tgatgagcat atcgcgcgga taaattttca
      301 atgcggataa ccgcgtccga ctctcttcaa tctgctgcgt gctcaatgtt accgggctgt
      361 ggttaatcac tctggactta acgctgccgt cttccagaag cacgtcaact tcaagcaacc
      421 cgttgatatc ataactaaag cgaatatcaa tcgactgata agccccggtt ttcttcaacg
      481 gcacatcgaa ggattcaacc agaatgttat ttttaacttt gtggttttct ccctgataga
      541 cgttaaccgt aattgaatcc tgctccgggt gcatggtcga gtaagtttct acacgcgaca
      601 ccggtactgt ggtgtttcgt tcaataatcg gcgagaaaat gccggaaacg ccctggcggt
      661 taacttcaac gcccaacgag taagggcaaa tatcagtgag gattacctct tcaatatctt
      721 cactgcgtaa gcggcaggcg gcctggattg ctgcgcccag cgcgacaatg gtgctcggat
      781 cgtaactttg atacggtaat ttgccaaaca gacgcacggc gattcgctgc accagcggca
      841 tttgtgacgc gccgccaacc agcaccagac tgtcgatttg actcggcttc agacgcgcat
      901 cgcgcaacgc ctgttcaatc ggcactcgca agcgattgag cagcggcaac cacaaatctt
      961 ccagttcgtt ctcgtaaaat tcgcattccc gcgtttcttc ctgatactgc cagcgaatgt
     1021 gcaatggcga ttgattgcta cattttgccg cttccacaca ggcgtacaag gctgccagtt
     1081 cactctcgtt aagcgtggtc ctggcaactt ccgcgcgttt taaaacctca tcgaccagca
     1141 tatgggtaaa atcttcgcca ccgaggaagc tatcgccagc ggaggcgtga atttcaatca
     1201 ccggcgtggc gtactcaagc accgtaacgt caaacgtgcc gccaccgaga tcaaaaacca
     1261 gcgagcgggt attttgttgc gtatgcaggc catatgccat cgcagctgct gtgggttcat
     1321 taattaagcg taccgcattt aacccggcta actccgcagc taaacgggta tgcttgcgtt
     1381 gttcatcgct gaaataagcc ggaacggaga tcaccacatc tttaatcgga cgttgcagaa
     1441 attcttcggc atcttctttt aatgagcgta ataccaaaga ggacagttct ggcgcgttaa
     1501 atgtgtcgct gcctaaccgc cagttggtat tactgcccat tgcgcgttta aataacgctg
     1561 ccgttttatc cggatgcgaa gtacgccgtg atacagccgg ttttccgact aaaatatgat
     1621 tattttcatc catgctaatt atggatggtg ttaaatattc accgaactta tttggaatta
     1681 attgcgcggc accgtctttc cagacggcaa ttaaactatt ggtagtgccg agatcgatac
     1741 caatggtgag ttctgcatta tccattgcat ttatatcttg aaagaaaaag agataaacag
     1801 attaagacca cccaggtaac gcgtggtgat cttaatcaat gacgtgtgtt aagcgtaaaa
     1861 tttcagacga tcagccagta aatcaacaaa gccctgacga tcaacatcga ccattacagt
     1921 ggcattcggt ttattgtcag tcagataata ataatcaaca accgtcatac cctgggtata
     1981 tttcccctgt gtttccacgc caacccagcg ctcaacagtg gtaaacaact cgggtttcaa
     2041 cagccaggcg atggtgcatg ggtcatgcag cggtgcaccg acaaagcccc atttttcgtc
     2101 tttatgatat tcgaggaaga aatccagcag ttcggcaaca atggttgaaa cagggttgcc
     2161 aatcgcgcgg aaacgctcgg tgtcttcaat gtggatttgc gctttatgag taacatccag
     2221 accggccatc accactggga tccctgactg gaagacaatt tctgccgctt ccgggtcaac
     2281 gtaaatatta aattcagccg caggcgtcca gttaccaagc cccattgcgc cgcccataat
     2341 cacgatacgg gcaattttgc tatgcagttc cggatggcta ttgagcagca aggcaacgtt
     2401 agtttgcggt ccggtagaca caatcgtgac aggttccgca ctttcgcgca gcgttttcgc
     2461 catcagctct accgccgtac agttttgcgg tgcgaacgtc ggttccggta atgccgggcc
     2521 atccagaccg ctttcgccgt gcacattgtc cgcgataatc aactcacgca ttaacggttt
     2581 taccgcgccg cctgctaccg gaatatcggt gcgattaagc aaggtcagca tacgcagaac
     2641 attgcgtaag gttttttctg gtgtctggtt tccggcggaa gacgtaattg ctttgacatc
     2701 aagctctggt gaggcgaggg cgagaactat tgcgatagcg tcgtcatgac ctgggtcgca
     2761 atctaacaga attggcagtg ccattgttgc tccttgttgt gtgcttcttt gcgacaaggg
     2821 taacgccagg atgtaacaga tacgaggggc gaaacgataa agcgtgagat ggcgcgcact
     2881 tgggtatgcg cgccagagtg attaatgcag gattttcgcg aggaagtctt tcgcgcggtc
     2941 cgatttcgga tcatcgaaga aagcgtcttt cggcgagtct tcgacaattt taccctcgtc
     3001 cataaagatc acccgattcg ccactttgcg ggcaaagccc atttcgtggg tcaccaccat
     3061 catggtcatt ccttcgttcg ccagttccac catcacgtcc agcacttcgt tgatcatctc
     3121 cggatccagc gccgatgtcg gttcgtcgaa cagcatcgca ataggatcca tacacaacgc
     3181 gcgagcgatt gccacacgct gctgctgacc gccggaaagc tgcgccggaa atttattggc
     3241 atgtgcggaa agtcccacgc gctccagcaa tttcaatgct ttttcacgag cgggcgcttt
     3301 atcgcgttta agcactttca cctgcgccag agtcaggttt tcaataatcg acaaatgggg
     3361 gaacaactca aaatgctgga acaccatccc gacgcgggaa cgcagctttg ccagatcggt
     3421 tttcttgtca ttaaccacga taccatcgac ggtgatttca ccttgctgca ctggttcgag
     3481 gccgttgacg gttttaatca gcgttgattt gccggaacca gacgggccgc aaaccaccac
     3541 cacttcgcct tttttcactt cggttgagca gtcggtcagc acctgaaagt gaccatacca
     3601 ttttgaaaca tttttcaggg taatcattat gctgtccttc ttttcaagta gctgaccaac
     3661 aacgacgcgc taagactaat aacgaaataa acaaatccgg caaacaggat catctcaacc
     3721 tgcgtaccat cacgctcacc aatggttgag gcggtacgga agaaatcggc cagagataac
     3781 acatacacca gtgaggtatc ctggaacagt acgatgccct gagtgagcag aagcggcacc
     3841 atcgcgcgga acgcctgcgg cagaataatc agtttcatcg actgccagtg agtcattccc
     3901 aacgccagcg cggcgctcga ctgaccacga gaaatacttt gaataccagc acggataatc
     3961 tctgaatagt aggccgcttc aaacatcgaa aacgccacca tcgccgaaat taaacggata
     4021 tcattttttg gtgataatcc cagcacgttt tgcagaaaac ctggcacgat caggtaaaac
     4081 cacagcaaaa ccataactaa aggaatcgag cggaatatgt taacgtaggc tttggcaaac
     4141 cacgctacgg gcgcaaagct ggacaagcgc atcaccgcca gcatggtgcc ccacaaaata
     4201 ccaatcacga ctgccgtgac ggtgattttc agcgtgatca ccagcccgtc gagcagatat
     4261 ggcagggaag ggacaatgga actccagtca aactcgtaca ttatttgccc cccatgttgc
     4321 caggcaggcg aactttacgt tcaaccagcg tcattaccag catgataatc gcgttaatca
     4381 acacatacgc cagcgtaata gcggtaaacg actcccaggc gtgggctgag taatcaagca
     4441 atttacccgc ctgcgccgcc atatccacca gaccaatagt cgaggcgatg gcggagtttt
     4501 tcaccaggtt catcatctcg gaagtcatcg gcgggacgat aacgcgataa gcattaggca
     4561 gcagtacgta tcgataagcc tgcggtagcg tcaggcccat cgccagcgcg gcattttttt
     4621 gcccacgcgg caacgactga atggcggcgc gtacctgttc gcaaacacgg gcggcggtaa
     4681 acagccccag gcagagcatg gatgaaagga aaaactgaat attgggatcc agctcggcct
     4741 taaaccacat gccgattttc tccggcaaca gctccgggat caccagatac caggtaaaga
     4801 attgcacaat caacggcacg ttgcggaaca gttcgacata caacgtaccc agaccggaga
     4861 gaaaacggtt tggaacggta cgtaaaatgc cgaaaaatga accgacgagg aaagcgataa
     4921 tccaggcgca gatcgacaaa gcgatcgtca cctgaaaacc actccagatc caaccaagat
     4981 aggtggtgtt gccgaacggg gcttgttgta aaaaaatacc ccagttccag tctatagaca
     5041 taaatctact ccagaaaaaa gagggtagca gcgttaactg ctaccctcga agattgttac
     5101 ccagcgtatt gcggttttca ggcccgatgg ggaacgacca tcaggcgtat agtctgtccg
     5161 tgctacgtaa caatcgagag ggctggaatt tccgcccctg gttcttgtaa ttagttcagt
     5221 gctttgtcat tcggttcttt gaacagtgct ttcatttcgt ctgacagttc gaaattcatg
     5281 ttcaggtttt tcggcggaat tggatttttg aaccatttat caaaccattt ttccgcttca
     5341 ccggaagtct gcacctgagc gatagtgtca tccatcagct ttttgaactg cggatcatct
     5401 ttacgcaaca tgcaaccgta ggcttcctga gactgcggct tgccgacgat atcccagttg
     5461 tctggtttct tcgctttcgc acgttcaccg gccagcaggg catcatccat cataaaggca
     5521 acggcacgac cgctttccag ggtgcggaaa gagtcaccgt gatctttggc gctgatgatg
     5581 cgcatattca ttttttgctc ttcattcagt ttgttgagca aaacttcaga ggtagtgccg
     5641 gaagtgacga ctacggcttt gtctttcagg tcggcaaaat ctttgatatc gccgcccttt
     5701 ttggtcagca ggcgcgtacc gaccacgaaa atagtatcag agaaagccgc ctgtttttgg
     5761 cgttcgacgt tgttggtggt agaaccacat tcaaaatcga aagtgccgtt ttgcagcagt
     5821 ggaatacggt tttgtgaggt aatcggaatc agttttacct gcaagtccgg tttgttgagt
     5881 ttctttttca ctgcttcaac aatggcgttg gagtaatcct gcgagtaacc caccactttt
     5941 tgctgattgt cgtaataaga gaaaggtact gaagattcac ggtgaccgac gacaatcaca
     6001 ccgtttttgg cgattttgtc cagcgtactg cctgctgccg gggcggcgtc atctgcctgt
     6061 gccagtcctg cggaaagcgc cagggcgagg attgctgtgg caggtttacg taattgcata
     6121 tccaactcct ttatcttctg cgttaagaac gcatggatac ccgttgtgag tgtttgtgtt
     6181 gttatcgtct gcaactttat tgtgcagtgt tgtgcctgtt aggtaacatt tagtttggct
     6241 aaatgtaaag atattgctgt tttattgttt gtttttgcga gatgcgccgc accattccga
     6301 agcaaaattc ttaaaccgca cctttttggt gctaccgctg atttgtggtg gtgcaacctg
     6361 gttgcgtcgc ggctgatttc atcgcttaca cagtaaataa agcaacttac atgccaaaaa
     6421 atacttaggg gcaaattaat tacgattgta attttaggct gaaaaagtca tatttcggac
     6481 agaattatct tttcggcatg ggacgtttgc cgggatgata actgcacacg ggaaaaatgc
     6541 tttttacctg tcttttcaag gaagaaacac cttatgtctg catcttcggt taagccgtta
     6601 aatgttcaat tacccgcaat aacccttatc ctttttgcac tctgtattgg gatattttgt
     6661 tatctcgcac aatggatgag ttatgaagag gtcgatcaat ccgcacttat ccatctcggc
     6721 gcaaacgttg cgcctcttac tttgtcgggt gaaccctggc gcttattgag cagtatcttt
     6781 ctgcacagta gtgtttctca tctgctgatg aatatgtttg cattcctggt cgtgggaggc
     6841 gtggcggaac agattctggg gaaatggcga ctcctgatta cctggttatt ctccggcgtc
     6901 tttggtgggc tcatcagcgc ctgttatgcg ttacgcgata gtgatcagat agtcatcagc
     6961 gttggggcat ccggggcaat tatgggaata gctggcgctg cgatagcaac acagcttgct
     7021 tcaggtgcgg gcacatacca taaaaaccag cggcgagtat tttctctgtt gggtatggtg
     7081 gcgctgacac tgttgtatgg tgcccggcaa acaggaatag ataatgcttg tcacattggc
     7141 ggcctgattg cgggtggcgc gttggggtgg ctgagcgcgc gtttatctgg gcaaaaccga
     7201 ctcgttacgg aaggcgggat tattgttgcg ggcagtcttc ttctgaccgg ggctatctgg
     7261 cttgcgcagc agcagatgga tgagtcagtt ttacaagtca ggcaaagcct gcgtgaagcg
     7321 ttttatccac aggagattga acaagagcga cggcaaaaaa agcagcagtt agcggaggaa
     7381 cgcaacgccc tcagggaaac attatccgct ccggtaagtc gtgaacaggc cagtggtgat
     7441 ttgctcgctg agattgccga tatccatgat atggcgatca gtcgggatgg taatatgttg
     7501 tatgccgcaa ttgaaaacac gaacagcatt gttgttttcg acctcggaca aaagaaaatc
     7561 ctgcatacct ttacagcgcc catagcgaaa gaaaagtcag tcaaacattg tggtggctgt
     7621 aaagatcagg gcgtcagatc gctggcatta agcccggatg aaaagttgat ttatgcgact
     7681 tcatttgaag cgaatgcgtt atcggtcatt aacgtggcga caggggagat tattcagtcg
     7741 attaccaccg gtgcacatcc tgacagcctt atcctctcgc gtgatggcac aaaagcctgg
     7801 gtgatgaatc gcaccagtaa tagtgtgtca gcgattgatc tggtgactta tcagcatgtg
     7861 gcggatatcc cgctggagaa atacgacggg gcggggacga gcggtaaacc aggcgcctgg
     7921 gttatggcac tttccccgga tgaaagaaca ttactggttc cgggagcagg cagaggtaac
     7981 atcgtgcgga tcaataccat cacgcatcag aaagaagact ttcccgcagg tgatgcgcgt
     8041 ggaacgatat cggcgatgcg ttttcgacct gaaaacggcg aggttatttt tgcagatagt
     8101 caggggattt cacgtataag cgtaggggat caacaagcca gcattatgac gcaatggtgt
     8161 agcaggagcg tttattccgt tgagggtatt agcccggacg gtcaatattt agcgttggtg
     8221 tcatatggcc tgcaaggtta tgtcatcctg ctcaatatta atgccgggca gattattggc
     8281 gtttatcctg ccagctacgt taatcacctt cgt
//
LOCUS       DAZPEB010000171         8252 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZPEB010000171 DAZPEB010000000
VERSION     DAZPEB010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8252
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.174"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            159..989
                     /locus_tag="ACHURJ_003371"
     CDS             159..989
                     /locus_tag="ACHURJ_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR1282190.1"
                     /translation="MKSVNVPRKSSNLLKRRLKIAEIVATQGEIKVDDLSAQLGVSGV
                     TIRGDLSYLEQQGYLKRSFGGAIATPLSSEPEAIQPIAPLSLAQQMEIARHCARLIRD
                     RDTLFLGHGTICRKIIPLLSEVKKLRLITNDPEHALLANQFIDGEIILAGSEMLRPDT
                     ALAGKPLEQALQHFTITHSILEINHLDADGTLNINVPRLAAAWQLCFDHAQNKTVIVA
                     ADPAPSTAAIGHLHQAENVIVSRSINEQYQQQLQSADFVILYTSNECFTWSNRERLQE
                     "
     gene            1021..1956
                     /locus_tag="ACHURJ_003372"
     CDS             1021..1956
                     /locus_tag="ACHURJ_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFR1282191.1"
                     /translation="MGELLAEFLSRNPHQKFTQPGEFIGPFPSGAPAIFAAQVAKLSH
                     RAIFFGCVGNDDFGRLIIERLRHEGVITDGIHVMNNAVTGTAFVSYQNPQQRDFVFNI
                     PNSACGLFTAEHIDKDLLKQCNHLHIVGSSLFSFRMIDVMRKAITTIKSAGGTVSFDP
                     NIRKEMLSIPEMAQALDYLIEYTDIFIPSESELPFFARHKNLSEEQIVSDLLHGGVKH
                     VAIKRAQRGASYYKLKNGTLHAQHVTGHDIEIIDPTGAGDCFGATFITLFLSGFPAHK
                     ALQYANASGALAVMRQGPMEGISSLADIEDFLQQH"
     gene            2049..2291
                     /locus_tag="ACHURJ_003373"
     CDS             2049..2291
                     /locus_tag="ACHURJ_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFR1282192.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLAAAYLPDVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            2281..3732
                     /gene="panF"
                     /locus_tag="ACHURJ_003374"
     CDS             2281..3732
                     /gene="panF"
                     /locus_tag="ACHURJ_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFR1282193.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVETLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            3744..4625
                     /gene="prmA"
                     /locus_tag="ACHURJ_003375"
     CDS             3744..4625
                     /gene="prmA"
                     /locus_tag="ACHURJ_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFR1282194.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            4954..5919
                     /gene="dusB"
                     /locus_tag="ACHURJ_003376"
     CDS             4954..5919
                     /gene="dusB"
                     /locus_tag="ACHURJ_003376"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFR1282195.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            5945..6241
                     /gene="fis"
                     /locus_tag="ACHURJ_003377"
     CDS             5945..6241
                     /gene="fis"
                     /locus_tag="ACHURJ_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFR1282196.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            6327..7211
                     /gene="yhdJ"
                     /locus_tag="ACHURJ_003378"
     CDS             6327..7211
                     /gene="yhdJ"
                     /locus_tag="ACHURJ_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFR1282197.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKIPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYDPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDII
                     LDPFAGSFTTGAVAVSSGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            7295..7474
                     /locus_tag="ACHURJ_003379"
     CDS             7295..7474
                     /locus_tag="ACHURJ_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFR1282198.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(7477..8139)
                     /gene="acrS"
                     /locus_tag="ACHURJ_003380"
     CDS             complement(7477..8139)
                     /gene="acrS"
                     /locus_tag="ACHURJ_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFR1282199.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIINGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
BASE COUNT         2051 a         1976 c         2060 g         2165 t
ORIGIN      
        1 tctgcataaa tatgaataaa ttcatttatt tttcaatgaa ttaatctatt tttattgcga
       61 tttttgaccc atgattttct tttcgaattg tcgatattat tgaaggataa ctttcaaaga
      121 aaaatacaag gggctatagt gttacgcatt aaaggccaat gaagagtgtt aacgtgccga
      181 gaaaatcgtc taacctactg aaacgcagat taaaaattgc agaaattgtt gccacccaag
      241 gtgaaatcaa agttgatgac ttatctgcgc aacttggtgt ctctggcgtg acgatccgcg
      301 gcgatctcag ttatctggag caacagggat atttaaagcg atcatttggc ggtgctattg
      361 cgacaccact ctcctctgaa ccagaagcga tacaaccgat cgcgccattg tctctggcac
      421 aacaaatgga aatagcacga cattgcgcac ggttaattcg cgatcgtgac acgctatttc
      481 ttggacatgg gacaatctgc cgtaagatca ttccattgct cagcgaagtg aaaaaactgc
      541 gtctgatcac caacgatccc gaacatgcgc tgctggcaaa tcagtttatt gatggcgaaa
      601 taatactggc tggcagtgaa atgctgcgcc ccgacaccgc actggccgga aaaccgcttg
      661 aacaggcact tcagcatttc accattacac atagcattct ggaaatcaac catctggatg
      721 ctgatggtac tttaaatatc aacgttccca gactggccgc tgcctggcag ctctgtttcg
      781 atcatgccca gaataagacg gttatcgttg ccgccgatcc tgcgccatca acggctgcta
      841 tcgggcattt gcatcaggca gaaaatgtga tcgtcagccg cagcattaac gaacaatatc
      901 agcaacaatt gcagtcggct gatttcgtta tcctttatac cagtaatgag tgctttacct
      961 ggtccaatcg cgaaaggctt caggaatgag aggggttttg tgacaataac tatctgcgct
     1021 atgggcgaat tgctggccga gtttttgtcc cgcaacccac atcaaaaatt cactcagcct
     1081 ggggagttta tcgggccatt tcccagcggt gcgccagcaa tttttgctgc tcaggtggca
     1141 aaactgtccc atcgggccat cttcttcgga tgtgttggta atgatgattt tggccgactc
     1201 attatagagc gcctccgtca tgaaggtgtc attaccgatg ggatccatgt tatgaacaat
     1261 gccgtcacag gtacggcgtt cgtgagttat caaaatcccc agcagcgcga tttcgtcttt
     1321 aatatcccta acagcgcctg cggcttgttt actgctgagc acattgataa ggatctgcta
     1381 aaacagtgta accatctcca tattgtgggc tcatcattgt tctcatttcg catgatcgat
     1441 gtcatgcgta aagcaataac gacgatcaaa tcggctggcg gcaccgtttc tttcgatccc
     1501 aatattcgca aagagatgct gagcattcct gaaatggcgc aggctctcga ttatttgatt
     1561 gaatatacgg atattttcat ccccagcgaa agcgaactcc ctttcttcgc gcgtcacaaa
     1621 aatctgtcag aagaacagat tgttagcgat cttctccacg gcggcgtaaa acatgtggcg
     1681 ataaaacgcg cccagcgtgg ggccagctat tacaagctta aaaacggtac attacacgcc
     1741 cagcatgtta caggccacga tatcgaaatt atcgatccaa cgggtgcagg cgactgcttt
     1801 ggcgcaacgt ttatcactct tttcttatcc ggtttcccgg cacataaggc actgcaatat
     1861 gcaaatgcca gcggcgcgct cgccgtaatg cggcaaggtc cgatggaagg gatatcctca
     1921 ctggctgaca ttgaagactt tttgcagcag cactgaacgg caattttctg tcagattatt
     1981 ttccctgcct gaagagttgc cgtagaattg cccgtttctc gtatcccccc atgaggagga
     2041 acccttccgt ggacactcgt tttgttcagg cccataaaga ggcgcgctgg gcgctggggc
     2101 tgaccctttt gtatctggca gtttggttag cagccgctta cttacctgac gttgcccccg
     2161 gttttaccgg ctttccgcgc tggtttgaga tggcctgcat cctgacgccg ctgctgttta
     2221 ttggactgtg ctgggcgatg gtgaaattta tctatcgcga tatcccactg gaggatgacg
     2281 atgcagcttg aagtgattct accgctggtc gcctatctgg tggtggtgtt cggtatctct
     2341 gtttatgcga tgcgtaaacg gagcaccggt accttcctta atgagtattt cctcggcagc
     2401 cgctctatgg gcggtattgt gctggcgatg acgctcaccg cgacctatat cagtgcaagt
     2461 tcgtttatcg gcgggcctgg agctgcttat aaatacggcc tgggttgggt attgctggcg
     2521 atgattcagc ttcctgcagt ctggctttcg ctcggtattc tcggcaagaa gttcgcgatt
     2581 cttgcgcgcc gctacaatgc agtgacgctg aacgacatgc tgtttgcccg ctaccagagt
     2641 cgtcttctgg tgtggctggc gagtttgagt ttgctggttg cgttcgttgg ggcaatgacc
     2701 gtgcagttta tcggcggtgc gcgcctgctg gaaaccgcgg cgggtattcc ttatgaaacc
     2761 gggctgctga ttttcggtat cagcattgcg ttgtataccg cctttggcgg ctttcgcgcc
     2821 agcgtgctga acgacaccat gcaagggctt gtgatgctga ttggcaccgt tgtgctgctt
     2881 attggcgtgg tacatgccgc aggtggttta agtaatgcgg tagagacgct gcaaaccatc
     2941 gatccgcaac tggttacgcc acaaggcgct gacgatattc tgtcgcctgc ctttatgacg
     3001 tcgttctggg tactggtgtg ctttggcgtg attggcctgc cgcatactgc ggtgcgctgt
     3061 atctcttata aagacagcaa agcggtacac cgggggatca tcatcggtac gattgtagtc
     3121 gcaatcctga tgttcggtat gcacctggcc ggagcgttag gtcgtgcagt gatccccgat
     3181 ctcaccgtac cggatctggt gatcccgacg ttaatggtaa aagtgctgcc accgtttgct
     3241 gccgggatct ttctggctgc acctatggct gcgatcatgt cgacaattaa cgcccaattg
     3301 ctacaaagtt ccgctacgat cattaaagat ctctatctga atatccgtcc ggatcaaatg
     3361 caaaacgaga cgcgtctgaa gcggatgtcg gcggtaatta cgttagttct cggcgcgttg
     3421 ctgctgcttg ccgcctggaa gccgccagaa atgatcatat ggctgaattt gctggccttc
     3481 ggtgggttgg aagccgtttt cctgtggccg ctggtgctgg gtctttactg ggaacgcgcc
     3541 aacgccaaag gcgcgctaag tgcgatgatc gttggcggcg tgctgtatgc cgtactcgcg
     3601 acgctgaata ttcagtacct gggcttccac cctatcgtgc cctcgttact actaagtttg
     3661 ctggctttcc tggtcggaaa ccgtttcggt acatccgtcc cgcaagctac cgttttgact
     3721 actgataaat aaagagtttt gccatgcctt ggatccaact gaaactgaac accaccggcg
     3781 cgaacgctga agatcttagc gatgcgctga tggaagcggg tgccgtttct atcacttttc
     3841 aggataccca cgatacgcca gtatttgagc cgctgccggg cgaaacgcgc ctgtggggcg
     3901 acaccgatgt gattggtctg ttcgacgctg aaaccgatat gaacgacgtg gtggcgattc
     3961 tggaaaacca tccgctgctc ggcgcaggct tcgcgcataa aatcgaacaa ctggaagata
     4021 aagactggga gcgcgaatgg atggataatt tccacccgat gcgctttggt gaacgactgt
     4081 ggatctgccc aagctggcgt gatgtgccgg acgaaaacgc cgtcaacgtg atgttagacc
     4141 cagggctggc gtttggtacg ggtacccatc caactacctc tctgtgccta caatggctcg
     4201 acagcctcga tttaactggt aaaacagtca tcgactttgg ctgcggttcc ggcattctgg
     4261 ctatcgcggc gctgaaactg ggtgcagcaa aagccattgg tattgatatc gatccgcagg
     4321 cgattcaggc tagccgcgac aacgccgaac gtaatggcgt ttctgaccgt ctggaactct
     4381 acttaccgaa agatcagcca gaagaaatga aagccgacgt ggtggtcgcg aacatccttg
     4441 caggcccatt acgtgaactg gcaccgttaa tcagcgtcct gccggtttca ggcggtttgc
     4501 tgggcctttc cggtattctg gcaagccagg cagagagcgt ttgtgaagct tatgccgata
     4561 gcttcgcact ggacccggtc gtggaaaaag aagagtggtg ccgtattacc ggtcgtaaga
     4621 attaaccttc gcatcgccgt agggtgacgc ggggcgaagt gcgagcaagc tcacaaaagg
     4681 cacgtaaatt tgccggttat ttacgcaaat ttgcgtgcca aaattttcat tcataaagaa
     4741 aaattgagaa cttactcaaa tttctttgaa tgtaaatttt agtcactatt ttctaatatg
     4801 atgattttta tgagtaatta tcgcaccacg ctcattttaa atgcaattct ttgatccatc
     4861 tcagaggatt ggtcaaagtt tggcctttca tctcgtgcaa aaaatgcgta atatacgccg
     4921 ccttgcagtc acagtatggt catttcttaa ctcatgcgca tcggacaata tcagctcaga
     4981 aatcgcctga tcgcagcgcc catggctggc attacagaca gaccttttcg gacgttgtgc
     5041 tacgagatgg gagccggatt gacagtatcc gagatgatgt cttctaaccc acaggtttgg
     5101 gaaagcgaca aatctcgttt acggatggtg cacattgatg aacccggtat tcgcaccgtg
     5161 caaattgctg gtagcgatcc gaaagaaatg gcagatgcag cacgtattaa cgtggaaagc
     5221 ggtgcccaga ttattgatat caatatgggt tgcccggcta aaaaagtgaa tcgcaagctc
     5281 gcaggttcag ccctcttgca gtacccggat gtcgttaaat cgatccttac cgaggtcgtc
     5341 aatgcagtgg acgttcctgt taccctgaag attcgcactg gctgggcacc ggaacaccgt
     5401 aactgcgaag agattgccca actggctgaa gactgtggca ttcaggctct gaccattcat
     5461 ggccgtacac gcgcctgttt gttcaatgga gaagctgagt acgacagtat tcgggcagtt
     5521 aagcagaaag tttccattcc ggttatcgcg aatggcgaca ttactgaccc gcttaaagcc
     5581 agagctgtgc tcgactatac aggggcggat gccctgatga taggccgcgc agctcaggga
     5641 agaccctgga tctttcggga aatccagcat tatctggaca ctggggagtt gctgccccca
     5701 ctgcctttgg cagaggttaa gcgcttgctt tgcgcgcacg ttcgggaact gcatgacttt
     5761 tatggtccgg caaaagggta ccgaattgca cgtaaacacg tttcctggta tctccaggaa
     5821 cacgctccaa atgaccagtt tcggcgcaca ttcaacgcca ttgaggatgc cagcgaacag
     5881 ctggaggcgt tggaggcata cttcgaaaat tttgcgtaaa cagaaataaa gagctgacag
     5941 aactatgttc gaacaacgcg taaattctga cgtactgacc gtttctaccg ttaactctca
     6001 ggatcaggta acccaaaaac ccctgcgtga ctcggttaaa caggcactga agaactattt
     6061 tgctcaactg aatggtcagg atgtgaatga cctctatgag ctggtactgg ctgaagtaga
     6121 acagcccctg ttggacatgg tgatgcaata cacccgtggt aaccagaccc gtgctgcgct
     6181 gatgatgggc atcaaccgtg gtacgctgcg taaaaaattg aaaaaatacg gcatgaacta
     6241 attcaggtta gctaaatgct tgattaaaaa ggcgctactc ggcatgggga agcgcctttt
     6301 ttataggtgt cacaaaggga gtgaccatga gaacaggatg tgaaccgacc cggtttggta
     6361 atgaagctaa gaccattatt cacggtgatg cgcttgccga acttaaaaag atccccgccg
     6421 aaagtgtcga tctgatcttt gccgacccac catataacat cggtaaaaat tttgatggtc
     6481 tgatcgaagc ctggaaagaa gatctgttta tcgactggct gtttgaagtg attgcagagt
     6541 gccaccgcgt tctgaaaaag cagggcagca tgtacatcat gaacagcacg gaaaacatgc
     6601 cctttatcga tctccagtgc cgcaagcttt ttaccatcaa aagtcgcatc gtctggtcat
     6661 atgacagttc tggagtacag gcgaaaaaac actacggctc catgtacgac cccatcctga
     6721 tgatggtgaa agacgcaaag aactacacat tcaacggtga tgctattctg gtagaagcca
     6781 aaaccggatc gcagcgcgcg ttgatcgatt atcgcaaaaa tcctccacag ccatacaatc
     6841 atcaaaaagt accgggtaac gtctgggatt ttccgcgcgt gcgttatttg atggatgaat
     6901 atgagaacca cccgacgcaa aaaccggaag ccttactgaa acgcattatt ctcgcctctt
     6961 ccaacccagg cgatatcatt ctcgacccgt ttgccggtag ctttactacc ggtgccgtag
     7021 ccgtctccag cgggcgaaaa ttcattggta ttgagatcaa cagcgagtac atcaaaatgg
     7081 ggcttcgacg gctggatgtc gcgtcgcatt actctgcgga agaactggcg aaagtgaaaa
     7141 aaagaaagac cggcaacctg tcaaaacgaa gccggttaag cgaagttgac cccgatctca
     7201 ttgcaaagta aaggaagtgt aagcctggtt ttcagattat tcatttcgtg tatatttcct
     7261 gccagacttg gttaaacatg cacaggctca ggtaatgatt cgcaagtatt ggtggctcgt
     7321 cgttttcgct gtcttcgttt ttctgtttga tactttactg atgcagtgga ttgaactgct
     7381 gacaacagaa acagacaaat gccgtaatat gaactcagtt aatccactaa aactggttaa
     7441 ctgtgacgaa ctgaattttc aggacagaat gtgaatttac atgacactta attcattcgt
     7501 ttgatgaatt aatttcgtta tgttttcatc tggcatgaac attcttaata cgttatcgac
     7561 cagcgcgggt gcttgtttat aaagatcata acccgccata ttcattaacc agttttgaac
     7621 aattccgctg aaggcaccat taataataat catcacaaca tctaaatcga ggttatttgc
     7681 tacacaacct tgttgctgac acgcctgcaa tacttcgcgg agagtctgcg gattaaagcc
     7741 catcttttcg cgtatcactc cctcggccag catctcatca ttaaattcac atttgtgata
     7801 taagattttc agcaacgcct gctggcgggg aattttggca atatattgca agccgacaat
     7861 caatttttca cgcaattgtt gaaacgggtc atgctctaat ccagccgtca agtgttcctg
     7921 gattaactcc cgcaatgaag gctgttgcaa ccacatctca ttaaacagtt gagtcttgtt
     7981 ttcgaagtgc cagtagatag cgccacgcgt aacgttagcg gcgtcggcaa tgtcgttgag
     8041 cgtcgtcttg cttacgccat gctgcgcaaa ctgggcgatg gcagtttcaa tcagttcttg
     8101 ccgggtcttc agagcttcgg ctttggttct ttttgccatg attaattatt caggaaataa
     8161 atatattcga cacagggtga gaaaatagcg aaggttaatc tatcatctaa tgtgtattta
     8221 tacgagaggg taacattgag ttgctataaa tc
//
LOCUS       DAZPEB010000172         8194 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZPEB010000172 DAZPEB010000000
VERSION     DAZPEB010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8194
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.175"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..815)
                     /locus_tag="ACHURJ_003381"
     CDS             complement(<1..815)
                     /locus_tag="ACHURJ_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR1282200.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSS"
     gene            907..993
                     /locus_tag="ACHURJ_003382"
                     /pseudo
     CDS             907..993
                     /locus_tag="ACHURJ_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1003..1083)
                     /locus_tag="ACHURJ_003383"
                     /pseudo
     CDS             complement(1003..1083)
                     /locus_tag="ACHURJ_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1294..1383
                     /locus_tag="ACHURJ_003384"
                     /pseudo
     CDS             1294..1383
                     /locus_tag="ACHURJ_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
     gene            1407..5921
                     /locus_tag="ACHURJ_003385"
                     /pseudo
     CDS             1407..5921
                     /locus_tag="ACHURJ_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
     gene            5922..7571
                     /locus_tag="ACHURJ_003386"
     CDS             5922..7571
                     /locus_tag="ACHURJ_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFR1282201.1"
                     /translation="MNWRRIVWLLALVTQPTLAEETPLQLALRGAQHDQLYQLSSSGV
                     TTVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIHTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            7576..>8194
                     /locus_tag="ACHURJ_003387"
     CDS             7576..>8194
                     /locus_tag="ACHURJ_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFR1282202.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKIQQGRLVVNGASMPQRVESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQV"
BASE COUNT         2002 a         1968 c         2300 g         1924 t
ORIGIN      
        1 gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg acaatggatt gttataaata
       61 ttaacatctg cataaatgtg cagattcatg atactgtctg gcaatgtgtt cagctgattg
      121 ttagggatat atagtgattg cagacgttgt ggaagttcgg gcagtctgtg tagttggttt
      181 tccccaacgt ttaaaagcct cagattttga ggaaggggcg gtagctcttg taatctgtta
      241 tcaaaagcac ttagttcctg tagttcacat ggtaattctg gtaaggcagt aagctggttg
      301 ttgccaacag aaagtttttg tagagccggg ggaagatcag gtagcgtctc caggctattg
      361 agaagggctg agagtgactg caaggaagat ggaagagaag gtaaacatgt taaaactctg
      421 ttaagtgaaa catcaagagc gaccaggtga ggcggaagag ccgggagcct gctcagtctg
      481 ttatcgctgg ctttaagtac agttaaggac ggagggagtt ctggcagaga gcgtagctca
      541 ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt atgggagaga gcgtaatctg
      601 agtaaactta aattgagggc tggctcttga aaagccagac atattttcag taatcgaact
      661 gcctgtgttc ggtcttctgt tgcagcacct tccttggccc agttatccca gatgcggtga
      721 taatccgtga tattttgctc ttctacggat aaacgggata tacatcctaa accacaaaag
      781 gagcggaaaa aagaaggatt gtgggcaggt ttcatttatt attgatcctc attgtttata
      841 tattaatatg ttcgtgcaaa tggagtgagg attctttaaa aaaacgctcc tgtaaatagt
      901 taagaacagg aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg
      961 ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc cctacctttt catcaagcca
     1021 gtagtctgcc caccactgca tcatttctct gcgcttatcg agatactgag catggttgta
     1081 aattcaacta acccagtgcg ttgacaaaaa attagcgcaa gaagacaaaa aatcaccttg
     1141 cgctaatgct ctgtatcagg tcactaatac cagctaagta gttgattcat agtgactgta
     1201 tatgttgtgc tttgtcgcat tttgtagtct attatttaat ctgtaacaac gataatacaa
     1261 tgaattttaa ttggtttttg tgattttgtc cttcttctga catggaagga cacccgatgg
     1321 atacccaacg attccaatcc caatttcatt ggcatttatc gtttaaattt tctggcgcga
     1381 tagcagcgtg cttgtctctg tctcttgtgg gaacagggct tgctaatgct gatgattcgc
     1441 ttccttccag taactatgcg ccgcccgccg ggggaacatt ctttttgctt gctgacagca
     1501 gttttagcag cagtgaagag gcgaaagtgc gactggaagc gccggggcgt gattatcggc
     1561 gctatcagat ggaagagtac ggcggcgtgg acgttcgcct gtatcgtatt cctgacccga
     1621 tggcattttt gcgccagcag aaaaacctgc atcgcattgt ggtgcaaccg caatatctgg
     1681 gcgacgggct gaacaatacg ctgacctggc tgtgggataa ctggtacggc aaatctcgcc
     1741 gcgtaatgca gcgtactttc tcttctcagt cacggcagaa tgtgactcag gcattacccg
     1801 aattacatct cggcaatgcc attattaaac cttcccgtta tgtacagaac aaccagtttt
     1861 ccccgctgaa aaaatatccc ttggtggaac agttccgtta tccactatgg caggctaaac
     1921 cggtcgagcc gcagcaaggg gtaaaactgg aaggcgcatc cagcaatttc atctcgccgc
     1981 agccgggtaa catttatatt cctctcggcc aacaagagcc gggactgtac ctcgtcgagg
     2041 cgatggttgg tgggtatcgg gcgacgacgg tggtgtttgt ttccgatacc gtggctctta
     2101 gcaaagtgtc aggcaacgag cttctggtgt ggaccgcggg taaaaaacag ggtgaagcga
     2161 agcccggctc agagatcttg tggactgacg gtcttggcgt gatgacccgc ggtgtgaccg
     2221 atgacagcgg taccttgcag ttacaacata tatcgccaga acgttcatac attctgggta
     2281 aggatgctga aggcggcgtt tttgtctccg agaacttctt ctacgaaagc gaaatctaca
     2341 acacccgctt gtatattttt accgatcgcc cgctatatcg cgcaggcgat cgtgtcgatg
     2401 ttaaagtgat gggccgcgag ttccacgatc cgttgcattc atcccccatc gtcagcgccc
     2461 cggcgaagct ttcggtgctg gacgctaacg gcagtctgtt gcaaaccgtc gatgtcacgc
     2521 tggatgcgcg caatggcggg cagggaagtt tccgcctgcc agaaaatgcc gttgccggag
     2581 gttatgagtt acgtcttgct taccgcaatc aggtctatag cagcagtttt cgcgtggcaa
     2641 actacatcaa gccacatttc gagattggtt tagctctcga caaaaaagag ttcaaaactg
     2701 gcgaagcggt cagcggcaaa ctgcaactgc tctacccgga tggcgagccg gtaaaaaatg
     2761 cccgcgtgca gttaagtttg cgcgctcagc aattatcaat ggtcggtaac gatttgcgtt
     2821 atgccggacg tttccccgtg tcgctggaag gcagcgaaac ggtgtccgac gccagcggtc
     2881 atgtggcgtt aaatctcccc gccgccgata aaccgagccg ttatttgtta accgtctccg
     2941 ccagtgacgg cgcggcgtat cgtgtcacca ccaccaaaga gatcctcatt gaacgcggtc
     3001 tggcgcatta ctcattaagt actgccgcac aatacagtaa tagcggcgag tcggttgtgt
     3061 tccgttatgc cgcgctggaa tcttcaaaac aggttcctgt tacgtatgaa tggttgcgtc
     3121 tcgaagaccg cacgagccat agcggagagc taccgtcagg cggcaaatcc tttaccgtca
     3181 atttcgctaa acctggcaac tacaatctga cattacgcga taaagacggc ttaattctcg
     3241 ctgggttaag ttatgccgtc agcggtaagg gcagcacggc gcatacgggt acggtagata
     3301 tcgtggcgga taaaacgctg taccagccag gcgaaaccgc gaagatgctg attacctttc
     3361 cggagccaat tgatgaagca ttattgacgc tggaacgcga tcgcgtggaa cagcagtcgc
     3421 tgctttcgca tccggcaaac tggctaacgc tacaacgttt aaacgatacc cagtatgaag
     3481 cccgggttcc agtgagcaat tcctttgcgc ctaacatcac tttttcggtg ctgtataccc
     3541 gtaatggtca gtacagtttt cagaacgccg ggatcaaagt tgccgttcct cagcttgata
     3601 tccgggtgaa aacggacaaa acccattacc agccaggtga actggtcaat gtcgaattaa
     3661 cctcgtcgct gaaaggtaaa cctgtttctg cgcagctaac ggtaggcgtg gtcgatgaaa
     3721 tgatctacgc gctgcaacca gaaatcgcgc cgaatatcgg caaatttttc tatccgctgg
     3781 ggcgtaacaa tgtgcgtacc agctccagtc tgtcgtttat cagctacgac caggcgctct
     3841 ccagcgagcc ggttgcgcct ggcgcaacta accgcagcga gcggcgagta aaaatgcttg
     3901 aacgtccacg gcgtgaagag gtggataccg cggcatggat gccgtcactc acaaccgata
     3961 aacaaggcaa agcgtatttc acgttcctga tgcctgattc gttaacccgc tggcgtatca
     4021 ccgcgcgtgg gatgaacggc gacgggctgg tcgggcaggg gcgtgcttat ctgcgttcgg
     4081 aaaaaaatct ctacatgaag tggagtatgc caacggtgta tcgcgtgggc gacaaaccgg
     4141 cggcaggact gtttatcttc agtcagcagg ataacgaacc ggtggcgctg gtgactaaat
     4201 ttgcaggcgc tgagatgcgc cagacgctga cgctgcacaa aggggcgaat tatatttcgc
     4261 tggcgcagaa tattcagcaa tctggcttgt taagtgcaga actgcaacaa aatgggcaag
     4321 tgcaggacag cattagcaca aaactgtctt ttgtggacaa cagcaggccc gttgaacagc
     4381 agaaaaatgt catgctcggc ggtggcgaga acgcgctgat gttgcccgag caggcgagta
     4441 atatccggct acaaagtagt gaaacgccgc aggagatttt ccgcaacaat cttgatgcgt
     4501 tagtcgatga accgtggggt ggggtgatca acaccggtag ccgtctgatc ccgctcagtc
     4561 tcgcctggcg ttcgcttgcc gatcatcaaa gtgccgccgc taacgacatt cgtcagatga
     4621 ttcaggataa ccgtctgcgg ctgatgcaac tggcggggcc cggagcgcgc tttacctggt
     4681 ggggtgaaga tggcaatggt gacgccttcc ttacggcatg ggcatggtac gccgactggc
     4741 aggccagcta ggcgctcggc gtaacgcaac aaccggaata ctggcagcat atgctcgaca
     4801 gttatgccga gcaggcggat aacatgccgt tattgcatcg ggcgctggtg ctggcgtggg
     4861 cacaggagat gaatctgccg tgcaaaacgt tgttgaaagg gttggatgaa gctatcgccc
     4921 ggcgcggaac taaagatgaa gatttctctg aggaagacat ccgcgatatc aatgacagcc
     4981 tgatcctcga tacaccggaa tctccactgg cagatgcggt ggcaaacgtc ttaaccatga
     5041 cgttgctgaa aaaagcgcag ttgaagtcca cggtgatgcc acaggttcag caatatgcgt
     5101 gggataaagc ggcaaacagc aatcagccgc tggcgcacac ggttgtgctg ctcaatagcg
     5161 gtggtgacgc tacccagacg gccgctattt taagtggttt gaccgctgag caatccacta
     5221 ttgagcgcgc gctggccatg aactggctgg cgaaatatat ggcgacaatg cctccagttg
     5281 ttttgcctgc gcctgcgggc gcatgggcta aacataagtt aactggaggg ggcgaatact
     5341 ggcgttgggt tggtcagggc gtgccggaca ttctctcttt tggtgacgaa ttatcgccgc
     5401 aaaatgtgca ggtccgctgg cgtgagccgg caaaaatggc tcaacaaagt aacattccgg
     5461 tgaccgttga acgccagttg tatcggctta tccctggtga agaagagatg agctttattc
     5521 tgcaaccggt gaccagcaat gagattgaca gcgatgcgct gtatctcgat gacatcacgc
     5581 ttaccagcga gcaggatgca gttctgcgct acggtcaggt ggaagtaccg ctgccaccgg
     5641 gagccgacgt tgagcgcaca acatggggca tttcggtcaa taaacccaac gccgcgaaac
     5701 agcaggggca attgctggaa aaagcgcgaa atgaaatggg cgaactggcc tatatggtgc
     5761 cggtgaaaga actgacggga acggtcactt tccgccattt gctgcgcttc tcgcaaaaag
     5821 ggcaattcgt tctgcctcct gctcgttatg tgcgttccta tgcacctgca cagcaaagtg
     5881 ttgcggcagg gagtgaatgg accgggatgc aggtgaaata agtgaactgg cgcagaatcg
     5941 tctggctatt ggcgttggtt actcagccaa cgcttgcgga ggagacgccg ttacaactgg
     6001 cactgcgtgg tgcgcagcac gatcaactgt atcagctgtc gtcatcaggc gtgaccacag
     6061 tctcagcatt acctgactcg ctcaccacgc cgttagggag tttgtggaaa ctgtatgttt
     6121 acgcctggct ggaggatacc catcaaccgg agcagcccta ccagtgtcgc ggaaactccc
     6181 cggaagaggt ttattgttgt caggctgggg agagcataac gcgcgatacc gcgctggtac
     6241 gttcctgtgg gctctatttt gcgccgcagc ggttgcatat tggcgcagat gtgtgggggc
     6301 aatactggca acagcgtcag gcgcctgcgt ggttagcgtc tcttactacg ctgaagccag
     6361 aaacctcggt aacggttaaa tcgctgcttg attcattagc gacgttacct gcgcaaaaca
     6421 aagctcagga agtcttactg gatgtggtgc tggacgaggc gaaaatcggt gtcgcatcca
     6481 tgctcggtag tcgggtacgg gtgaaaacct ggagctggtt tgccgatgat aaacaggaaa
     6541 tccgtcaggg tggttttgcc ggctggttaa ccgatggtac accgctgtgg gttactggca
     6601 gtggcaccag caaaacggta ctaacccgtt atgcgacagt gcttaatcgg gttttgccag
     6661 tgccgacgca ggttgccagt gggcaatgtg tagaggtgga gctgtttgcc cgctatccgc
     6721 tgaagaaaat cacagcggaa aagagtacga cggcagtaaa acccggcgta cttaatgggc
     6781 gctatcgtgt gacgtttact aatggtaacc acattacgtt tgtcagccac ggtgagacga
     6841 ccttactgag cgaaaagggc aaactgaaat tgcagtcgca tctcgatcgt gaagagtacg
     6901 ttgccagagt tctggatcgc gaggcgaaaa gtacgccccc tgaggcagcg aaagccatga
     6961 ccgtggcgat tcacactttt ttacagcaaa acgccaatcg ggaaggcgat tgcctgacca
     7021 tccccgacag cagcgccacg cagcgtgttt ctgcttcgcc agcgaccacg ggggcgcgaa
     7081 cgatgaccgc ctggacgcag gatctcatct atgcaggcga tccggttcat tatcatggca
     7141 gtcgagctac cgaagggaca ctttcctggc ggcaagccac ggcccaggcg gggcagggag
     7201 agcgttacga ccagatactc gcgtttgctt atcccgacaa cagccttagc cgctggggag
     7261 cgccacgctc aacctgccag ttattaccca aagcgaaagc ctggctggcg aaaaaaatgc
     7321 cgcagtggcg gcgtatatta caaggcgaga cggggtacaa cgaaccagac gtgtttgcgg
     7381 tctgtcgttt agtctcaggt ttcccctata ccgatcggca gcagaaacgg ctgtttattc
     7441 gtaacttctt tacgcttcag gatcggctgg atttaaccca tgagtatctg cacctggcct
     7501 ttgatggtta tcccaccggg cttgatgaga actatatcga aacgctgacc cgacaattat
     7561 taatggacta atgctatgcg aaagattttt cttccgttgt tactggtggc actttcgcct
     7621 gtcgctcaca gtgaaggtgt gcaggaagtc gagattgacg caccgctctc tggctggcat
     7681 ccggcagagg gcgaagatgc cagtttttca caatcgatca attacccggc gtcgtccgtc
     7741 aacatggcag atgatcaaaa tatctcagcg cagatccgcg gcaaaataaa gaattatgct
     7801 gcggcgggta aaattcagca gggccggctg gtggtcaacg gtgccagtat gccgcagaga
     7861 gttgaatccg atggttcatt tgcacgccct tatattttca ctgaaggcag caacagcgtg
     7921 caggtcatca gcccggatgg gcaaagccga caaaaaatgc agttttactc aacgccgggt
     7981 acaggaacga ttcgtgcacg tttacggctg gttctctcgt gggatacgga caataccgat
     8041 ctcgatctcc atgttgtcac gcccgatggc gaacacgcct ggtacggtaa caccgtgctg
     8101 aaaaacagtg gtgcactgga tatggatgtc acgacggggt acggacccga gattttcgcc
     8161 atgccagcgc cagttcacgg acgctatcag gtgt
//
LOCUS       DAZPEB010000173         8160 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZPEB010000173 DAZPEB010000000
VERSION     DAZPEB010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8160
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.176"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            171..506
                     /gene="eutS"
                     /locus_tag="ACHURJ_003388"
     CDS             171..506
                     /gene="eutS"
                     /locus_tag="ACHURJ_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFR1282203.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLISHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            519..998
                     /gene="eutP"
                     /locus_tag="ACHURJ_003389"
     CDS             519..998
                     /gene="eutP"
                     /locus_tag="ACHURJ_003389"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFR1282204.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            973..1674
                     /gene="eutQ"
                     /locus_tag="ACHURJ_003390"
     CDS             973..1674
                     /gene="eutQ"
                     /locus_tag="ACHURJ_003390"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFR1282205.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGGLHVRHEGQTMIAKAGDVMFIPKGSSIEFGMTSS
                     VKFLYVAWPANWQSL"
     gene            1671..2480
                     /gene="eutT"
                     /locus_tag="ACHURJ_003391"
     CDS             1671..2480
                     /gene="eutT"
                     /locus_tag="ACHURJ_003391"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001425897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFR1282206.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQKPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVLEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGERNDY"
     gene            2470..3486
                     /gene="pta"
                     /locus_tag="ACHURJ_003392"
     CDS             2470..3486
                     /gene="pta"
                     /locus_tag="ACHURJ_003392"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFR1282207.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSFIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YNIAQRLGGYRAVGPLIQGLAVPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            3525..3818
                     /gene="eutM"
                     /locus_tag="ACHURJ_003393"
     CDS             3525..3818
                     /gene="eutM"
                     /locus_tag="ACHURJ_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFR1282208.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            3925..4212
                     /gene="eutN"
                     /locus_tag="ACHURJ_003394"
     CDS             3925..4212
                     /gene="eutN"
                     /locus_tag="ACHURJ_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFR1282209.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            4224..5627
                     /locus_tag="ACHURJ_003395"
     CDS             4224..5627
                     /locus_tag="ACHURJ_003395"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFR1282210.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            5638..6474
                     /gene="eutJ"
                     /locus_tag="ACHURJ_003396"
     CDS             5638..6474
                     /gene="eutJ"
                     /locus_tag="ACHURJ_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFR1282211.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVAELLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPTVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            6464..6862
                     /locus_tag="ACHURJ_003397"
                     /pseudo
     CDS             6464..6862
                     /locus_tag="ACHURJ_003397"
                     /inference="COORDINATES: protein motif:HMM:NF025065.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
     gene            6911..8086
                     /gene="eutB"
                     /locus_tag="ACHURJ_003398"
                     /pseudo
     CDS             6911..8086
                     /gene="eutB"
                     /locus_tag="ACHURJ_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
BASE COUNT         1868 a         2152 c         2436 g         1704 t
ORIGIN      
        1 tattatttta tttatattca tgcgttgcat atgaaagttt atgcaccaca gcgaatatct
       61 ctccattctt agtgatctac ctcacctttt aaacgcgctt gccgaatttt gttatttact
      121 ctgacgaaaa attgtcacga tacacgaaag tttttcacag gcggcgactc atggataaag
      181 aacgcatcat tcaggaattt gtgcccggca aacaggtcac actggcgcat ctcatctcgc
      241 atcccggtga ggaactggcg aagaaaatcg gcgttccgga tgcgggcgca attggcatta
      301 tgacgctaac tcccggcgaa acggcgatga tcgccggtga tttagcgctg aaagccgctg
      361 atgtgcatat cggttttctc gaccgcttca gcggtgcatt ggtgatttat ggctcggtgg
      421 gcgcagtaga agaagcgtta tcacaaaccg tcagcggttt aggtcgctta ttaaattata
      481 cgctttgtga aatgaccaaa agttaatacg aggcattcat gaaacgaatt gcgtttgtgg
      541 gtagtgtcgg ggcaggaaaa acaacgttat ttaatgcgtt gcaggggaat tataccctcg
      601 ccagaaaaac acaggccgtg gaatttaatg ataagggcga tattgatacg ccgggtgaat
      661 attttagcca tccccgctgg tatcacgcct taattaccac gctacaggat gtagacatgc
      721 tgatctatgt ccatggcgca aatgacccgg aaagtcggtt gcctgccggg ttgctggata
      781 ttggcgtcag caaacggcaa atcgccgtca tcagtaaaac ggacatgcca gatgctgacg
      841 tcgccgcaac gcgaaagtta ttgcttgaga cgggtttcga agagccgatt ttcgagctaa
      901 acagccacga cccgcaaagc gtgcagcaac tggtggatta tctggcatct ctcaccaaac
      961 aggaggaagc aggtgaaaaa actcatcaca gcgaatgata ttcgtgaagc acacgcgcgc
     1021 ggcgaacagg caatgtccgt ggttctgcgc gccagcatta ttaccccgga agcccgtgaa
     1081 gtggcggatc tactgggttt taccatcacc gaatgtgatg aatcgatccc ggtaacggcg
     1141 tccgtgcccg ccagcgtgcc agccgacaaa accgaaagcc agcgcatccg cgaaaccatc
     1201 attgcccagc tgccggaagg ccagtttacc gaaagcctgg tcgcacagct gatggaaaaa
     1261 gtgatgaagg aaaaacagtc gctggagcag ggcgcaatgc agccgagctt caaatcggtg
     1321 accggcaaag gcggcatcaa agtgattgat ggcagcagcg tcaagtttgg tcgtttcgac
     1381 ggcgcagagc cgcattgcgt gggcttgact gatcttgtga ccggagacga cggcagcagc
     1441 atggccgccg gttttatgca gtgggagaac gcattcttcc cgtggaccct gaactacgac
     1501 gaaatcgaca tggtgctgga aggggggctg catgttcgcc acgaaggcca aaccatgatc
     1561 gccaaagcag gcgacgtgat gtttatcccg aaaggctcca gcatcgaatt tggtatgaca
     1621 tccagcgtga aattcctgta tgtcgcctgg ccggctaact ggcaatccct atgaaagatt
     1681 tcatcaccga agcatggcta agagcgaacc atacgctcag cgaaggcgca gagatccatc
     1741 tccctgcgga cagtcgcctg acgccctctg cccgggagtt gctggaaagc cgccatctgc
     1801 gcatcaagtt tattgacgag cagggccgcc tgtttgttga cgatgaacag cagaagccgc
     1861 agcccgttca tgggctgacc agtagcgatg aacatccgca ggcgtgctgc gaactgtgcc
     1921 gccagccggt ggcaaaaaag ccggatacgc tgactcacct gtcggcggaa aaaatggtcg
     1981 ccaaaagcga tccgcgtctg ggttttcgcg cggttctcga cagcaccatt gcgctggcag
     2041 tgtggctgca aattgaactg gcggaaccgt ggcagccgtg gctggcggat atccgttcgc
     2101 gtcttggcaa cattatgcgc gccgatgcgc tgggggaacc gctgggctgc caggcgattg
     2161 tcgggctttc tgacgaagat ctgcatcggc tttctcatca gccgctgcgc tatctcgacc
     2221 acgatcatct ggtgctggaa gccagccacg ggcgcgatgc tgcgttgctc aatctgctac
     2281 gcaccaaagt gcgggaaacc gaaaccgtag cggcgcaggt ctttatcacc cgcagctttg
     2341 aagtattacg cccggacatt ttgcaggcgc tgaaccgcct ttccagcacg gtctacgtga
     2401 tgatgatcct gagcgtaacg aagcagccgc taacggtgaa gcagatccaa caacgactgg
     2461 gggaacgcaa tgattattga acgttgtcgt gaactggcgt tgcgagcgcc cgccagagtg
     2521 gtttttccgg atgcgttaga tcaacgtgtg ctgaaagccg cgcaatattt acatcaacaa
     2581 ggtctggcaa cgcccattct ggtcgccaat ccgtttgaac ttcgtcagtt tgcgctcagt
     2641 cacggcgtgg cgatggacgg gctacaggtg attgatccgc atggcaacct cgcaatgcgg
     2701 gaagaatttg ctcatcgctg gctggcccgc gcgggcgaaa aaacgccgcc ggatgcgctg
     2761 gaaaaactta ccgaccctct gatgttcgcc gcagcaatgg tcagcgccgg taaagcggat
     2821 gtctgtatcg caggcaacct ctcttccacg gcaaatgtgc tgcgtgccgg attacgcatt
     2881 atcggcttgc agccaggctg taaaacgctc tctttcattt tcctgatgtt gccacagtac
     2941 agcggcccgg cgttgggctt tgccgattgc agcgtggtgc cacagccgac ggcggcgcag
     3001 ctggcggata tcgcgcttgc cagtgccgaa acctggcgcg ccatcaccgg agaagagccg
     3061 cgcgtggcga tgctgtcgtt ttccagcaat ggtagcgccc gtcacccctg tgttgctaac
     3121 gtccagcagg cgacagaaat cgtccgtgag cgcgcaccaa agctggtggt ggatggcgag
     3181 ttacagtttg acgccgcctt cgtgccggaa gtggcggcgc aaaaagcgcc tgccagcccg
     3241 ctacagggca aggccaatgt gatggttttt ccgtcgctgg aagccggaaa tattggttac
     3301 aacatcgcac aacgactcgg cggatatcgt gccgtcgggc cactgataca aggacttgcc
     3361 gtgccgatgc acgatctctc tcgtggttgt agcgtgcagg aaattatcga gctggcgctg
     3421 gtggcagctg tgccgcgtca gacagaagtg aaccgcgaaa gcagtttaca aacactggtt
     3481 gaatgaacgg tcccgttctg gaccctgata aagaggaaag cacaatggaa gcattaggaa
     3541 tgatcgaaac ccggggcctg gttgcactga ttgaggcttc tgacgcgatg gtgaaagctg
     3601 cgcgcgtcaa gctggttggt gttaagcaga ttggcggcgg cctgtgtact gccatggttc
     3661 gtggtgatgt tgcagcctgt aaagccgcga ccgatgcagg tgccgcagcg gcacagcgta
     3721 tcggcgagct ggtatctgta cacgttatcc cgcgtccgca cggcgacctg gaagaagtct
     3781 tcccgatcgg cctgaaaggc gatagcagca acctgtaatc accctgtgtg acgtcgattc
     3841 gggcgttacg ctctctcctt tttcatttta ccttctgcgg agggtagggg gagcttttac
     3901 ctgaaatacg ccacggaggc gggtatgaaa ctggcagtcg tcactggaca aattgtttgt
     3961 accgtacgcc atcacggact ggcgcatgac aaattgctga tggtggaaat gattgatcca
     4021 caaggtaatc ctgacgggca atgcgccgtc gccatcgaca atattggcgc gggaaccggg
     4081 gagtgggtgt tactggtgag tggcagttcc gcccgccagg cgcataaaag cgaaacgtca
     4141 ccggtcgatc tgtgcgtgat tggcattgtc gatgaggtgg tgtctggcgg tcaggtaatt
     4201 ttccacaaat aaggcagaac atcatgaatc aacaggatat tgaacaggtg gtgaaagcgg
     4261 tactgctgaa aatgcaaagc agtgacacgc cgcccgccgc cgttcatgag atgggcgttt
     4321 tcgcgtccct ggatgacgcc gttgcggcag ccaaagtcgc ccagcaaggg ttaaaaagcg
     4381 tggcaatgcg ccagttagcc attgctgcca ttcgtgaggc aggcgaaaaa cacgccagag
     4441 atttagcgga acttgccgtc agtgaaaccg gcatgggccg cgttgaagat aaatttgcca
     4501 aaaacgtcgc tcaggcgcgc ggcacgccag gcgttgagtg tctctctccg caggtgctga
     4561 ccggcgacaa cggcctgact ttaattgaaa acgcgccctg gggcgtggtg gcttcggtaa
     4621 ctccgtccac taacccggcg gcaaccgtaa ttaataacgc catcagcctg attgccgcgg
     4681 gcaacagcgt catttttgcc ccgcatccgg cggcgaaaaa agtctcccag cgggcgatca
     4741 cgctgctcaa ccaggcgatt gttgccgcag gcgggccgga aaacttactg gttactgtgg
     4801 caaatccgga tatcgaaacc gcgcagcgtt tgttcaagtt tccgggcatc ggccttctgg
     4861 tggtgaccgg cggcgaagcg gtagtagaag cggcgcgtaa acacaccaat aaacgtctga
     4921 ttgccgcagg cgctggcaac ccgccggtag tggtggatga aaccgccgac ctcgcccgtg
     4981 ccgctcagtc catcgtcaaa ggcgcttctt tcgataacaa catcatttgt gccgacgaaa
     5041 aggtactgat tgttgttgat agcgtagccg atgaactgat gcgtctgatg gaaggccagc
     5101 acgcggtgaa actgaccgca gaacaggcgc agcagctgca accggtgttg ctgaaaaata
     5161 tcgacgagcg cggaaaaggc accgtcagcc gtgactgggt tggtcgcgat gcggcgaaaa
     5221 tcgcggcggc aatcggcctg aacgtgccgc aagaaacgcg tttgctgttt gtggaaacca
     5281 ctgcagaaca tccgtttgcc gtgaccgaac tgatgatgcc ggtgctgccc gtcgtgcgcg
     5341 tcgccaacgt ggcggatgcc attgcgctgg cggtgaaact ggaaggcggt tgccaccaca
     5401 cggcggcaat gcactcgcgc aacatcgaaa acatgaacca gatggcgaat gctattgata
     5461 ccagcatttt cgttaagaac ggaccgtgca ttgccgggct ggggctgggc ggggaaggct
     5521 ggaccaccat gaccatcacc acgccaaccg gtgaaggggt aaccagcgcg cgtacgtttg
     5581 tccgtctgcg tcgctgtgta ttagtcgatg cgtttcgcat tgtttaagga gtaacagatg
     5641 gcgcacgacg aacaatggct caccccacgt ctgcaaacgg cggcaacgct gtgtaaccag
     5701 acgcccgccg cgacggaatc tccgctgtgg ctgggggtgg atctgggcac ctgcgatgtg
     5761 gtgtcgatgg ttgtcgaccg cgacggtcag ccggtggcgg tatgcctcga ctgggccgat
     5821 gtggtccgcg acggcatcgt ctgggatttc ttcggcgctg tcaccattgt tcgtcgccat
     5881 ctcgacacgc tcgagcagca attcggtcgc cgttttagcc atgcggcgac ttcatttcct
     5941 ccaggtacgg acccgcgtat ttccatcaac gtgctggagt ctgctggact ggaagtgagc
     6001 catgtgctgg atgagccaac cgcagttgcg gagctgctgc aactggacaa cgccggtgta
     6061 gtggatatcg gcggcggcac taccggcatt gccatcgtga aaaagggcaa ggtgacgtac
     6121 tcggcggatg aagcaaccgg cggacatcac atctctctga ctctcgccgg aaatcgccgt
     6181 atttcgctgg aagaggcgga gcagtacaag cgcggtcacg gtgaagagat ttggcctacg
     6241 gtgaaaccgg tgtacgaaaa aatggcggac attgtcgccc ggcatattga agggcagggc
     6301 attactgatt tatggctggc gggcggctct tgtatgcaac cgggcgtggc ggaactgttt
     6361 cgcaaacaat tcccggcgtt acaggtgcat ttaccgcagc acagtttgtt tatgaccccg
     6421 ctggcgatcg ccagtagtgg gcgagagaaa gcggaggggc tatatgcaaa atgaattgca
     6481 gaccgcgctc tttcaggcgt tcgataccct gaatctgcaa cgggtaaaaa catttagcgt
     6541 tccaccggtg acgctttgcg gtccgggctc ggtgagcagt tgcggacagc aagcgcaaac
     6601 gcgtgggctg aaacatctgt tcgtgatggc agacagcttt ttgcatcagg cagggatgac
     6661 cgccgggctg acgcgcagcc tggctgttaa aggcatcgcc atgacgctct ggccatgtcc
     6721 ggtgggcgaa ccgtgcatca ccgacgtgtg tgcagccgtg gcgcagttgc gtgagtcagg
     6781 ctgtgatggg gtgatcgcat ttggcggcgg ctcggtgctg gatgcggcga aagccgtggc
     6841 gttgctggtg acgaacccgg atagcacgct ggcagagatg tcagaaacca gcgttctgca
     6901 accgcgcttg ccgctgattg cctatgaaga tgactgcgtg acgcggctga ttcaggacga
     6961 tgttaacgaa acggcctaca accagattaa aaactggagc atcagcgaac tgcgtgagta
     7021 tgtgctgagc gatgaaacta gcgtggacga cattgccttt acccgcaaag ggctgacctc
     7081 ggaagtggtc gcggcggtag cgaagatttg ctccaacgcg gacctgatct acggcgcgaa
     7141 gaaaatgccg gtaatcaaaa aggccaatac caccatcggt attccgggca ccttcagcgc
     7201 ccgtttgcag ccaaatgaca ctcgtgacga cgtgcaaagt atcgccgcgc aaatctacga
     7261 agggctttcc ttcggggtgg gcgatgcggt gatcggcgtc aacccggtaa ctgacgacgt
     7321 ggaaaactta agccgcgtgc tggataccat ttatggcgtg atcgacaaat tcaacatccc
     7381 aactcagggc tgcgtactgg cgcacgtcac cacccagatc gaagcgattc gtcgcggcgc
     7441 gcctggcgga ctgattttcc agagtatctg tggcagcgaa aaagggctga aagagtttgg
     7501 cgtggaactg gcgatgctcg acgaagcgcg cgcagtgggt gcagagttca accgtatcgc
     7561 cggggaaaac tgcctctact tcgaaaccgg acaaggctct gcgctatccg ctggcgctaa
     7621 cttcggcgct gaccaggtga cgatggaagc acgtaactat gggctggcgc gtcattacga
     7681 tccgtttatc gtcaacaccg tggtcggctt tattgggccg gagtatctct acaacgaccg
     7741 ccagattatc cgtgctggct tagaagatca ctttatgggc aagctgagcg gcatctctat
     7801 gggctgtgac tgctgctaca ccaaccacgc tgacgctgac cagaacctca acgaaaacct
     7861 gatgatcctg ctcgccaccg caggctgcaa ctacatcatg gggatgccgc tgggtgatga
     7921 catcatgctc aactaccaga ccaccgcatt ccacgatacc gccactgtgc gtcagttact
     7981 caacctgcgc ccgtcaccag agtttgaacg ctggctggaa agcatgggca ttatggcaaa
     8041 cggtcgcctg accaaacggg cgggcgatcc gtcactgttc ttctgatgac gcggagataa
     8101 cacatcatgg atcaaaaaca gattgaagaa attgtacgca gcgtgatggc gtcaatggga
//
LOCUS       DAZPEB010000174         8156 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZPEB010000174 DAZPEB010000000
VERSION     DAZPEB010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8156
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.177"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..61)
                     /locus_tag="ACHURJ_003399"
     CDS             complement(<1..61)
                     /locus_tag="ACHURJ_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282212.1"
                     /translation="MNINYPAEYEIGDIVFTCIG"
     gene            132..197
                     /gene="yobI"
                     /locus_tag="ACHURJ_003400"
     CDS             132..197
                     /gene="yobI"
                     /locus_tag="ACHURJ_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HFR1282213.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            336..947
                     /gene="ruvA"
                     /locus_tag="ACHURJ_003401"
     CDS             336..947
                     /gene="ruvA"
                     /locus_tag="ACHURJ_003401"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFR1282214.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            956..1966
                     /gene="ruvB"
                     /locus_tag="ACHURJ_003402"
     CDS             956..1966
                     /gene="ruvB"
                     /locus_tag="ACHURJ_003402"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFR1282215.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(2016..2801)
                     /gene="znuB"
                     /locus_tag="ACHURJ_003403"
     CDS             complement(2016..2801)
                     /gene="znuB"
                     /locus_tag="ACHURJ_003403"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFR1282216.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLALDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLIAIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(2798..3553)
                     /gene="znuC"
                     /locus_tag="ACHURJ_003404"
     CDS             complement(2798..3553)
                     /gene="znuC"
                     /locus_tag="ACHURJ_003404"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFR1282217.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            3632..4564
                     /gene="znuA"
                     /locus_tag="ACHURJ_003405"
     CDS             3632..4564
                     /gene="znuA"
                     /locus_tag="ACHURJ_003405"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFR1282218.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLLGAKQV
                     TIAQLEDVKLLLMKSIHGDDDDHDHAEKSEEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4580..5902
                     /gene="mepM"
                     /locus_tag="ACHURJ_003406"
     CDS             4580..5902
                     /gene="mepM"
                     /locus_tag="ACHURJ_003406"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFR1282219.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            6022..6993
                     /gene="lpxM"
                     /locus_tag="ACHURJ_003407"
                     /gene_synonym="msbB"
     CDS             6022..6993
                     /gene="lpxM"
                     /locus_tag="ACHURJ_003407"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFR1282220.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMVGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLQIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRRPGEIQPYKRKDLYPIK"
     gene            complement(7082..>8156)
                     /locus_tag="ACHURJ_003408"
     CDS             complement(7082..>8156)
                     /locus_tag="ACHURJ_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HFR1282221.1"
                     /translation="SRSIENNYIKLLFSENGDLISLYDKRYGKEYITENMHSEIRAYH
                     EDAGFFAAWDFASNYRDGESYVLLAEKMTTVISGPKTTMTLIYHYNSSYLRFAFTLTQ
                     DSPRVDVQTFIDWHEPNVSLKVKFPVSVQTSLAQCQIQFGVIDRPTHSDDSFAFAKDE
                     IPAHHWVDLSDRETGIALIAKNKYGYRVKDQTLELTLLRSQHKPGEIVKTDNYDNFLI
                     NNFADIGKQKFIFSLFPHHGDYRVGGVINQAYIMNHPLQVVPVKPHPGELPPSLSFFA
                     IDTNSVIIETIKLAEDGDGIIVRLYESHGLEISAMLSWVCNYHYVQYVDLQENKLDDS
                     FYCNQYCNLEFKPFEIITLRLTQ"
BASE COUNT         2196 a         1914 c         2026 g         2020 t
ORIGIN      
        1 cacctataca tgtaaagacg atatctccaa tttcatattc agcagggtaa ttaatattca
       61 cgttgtcatt ccattgaaat agatacgaat atacgtaata gcataaaaaa attatttttt
      121 caggaggtgc aatgtatatt tttattacgc atttcttcac tgaatacgta atatttaaat
      181 atttgcttcc aatataacct gtagaataaa tcatactgcg ccatttttca gttcatcgag
      241 acacctcgca agttttcttc atccttcgct ggatatctat ccagcatttt tttatcatac
      301 agcattatct ttgattcatt acgcaggagc gtcatgtgat aggcagactc agaggcatca
      361 tcattgaaaa acaacccccg ctggtgttaa ttgaagtggg cggcgtaggc tatgaagtgc
      421 atatgccgat gacctgtttt tatgaactcc ctgaagcggg tcaggaagcg atcgttttca
      481 cccactttgt ggtgcgtgaa gacgcgcaac tgctgtacgg ttttaacaat aaacaagagc
      541 gcacattgtt caaagagttg atcaaaacca acggcgtcgg cccgaagttg gcgctggcga
      601 tcctctccgg aatgtcagcg cagcagttcg ttaatgccgt tgaacgtgaa gaagtggggg
      661 cactggtgaa actgccgggt attggcaaaa aaaccgccga acgcttgatt gttgaaatga
      721 aagaccgatt taaaggtttg catggcgatc tgtttacccc tgccgccgac ctggtactca
      781 catcacctgc cagcccggcg accgacgatg ctgaacagga agcggttgcc gcgctggtgg
      841 cgctgggcta taaaccacaa gaagcaagcc gcatggtgag caaaatcgct cgccctgacg
      901 ccagcagtga aacattaatt cgcgaagccc tacgcgccgc gttatgaggt aaaggatgat
      961 tgaagcagac cgtctgattt ctgccggtac cactttgccg gaagatgtag cagatcgcgc
     1021 cattcgccct aaattactgg aagagtatgt tggtcagccg caggttcgtt cacagatgga
     1081 gattttcatc aaagcagcga aactgcgcgg cgatgccctc gatcatttgt tgatttttgg
     1141 tcctccgggg ttgggtaaaa ctacgcttgc caacattgtc gccaatgaaa tgggcgttaa
     1201 tttacgcacg acttctggtc cggtgctgga aaaggcgggc gatctggcag cgatgctcac
     1261 taaccttgaa ccgcatgacg tgctgtttat tgatgagatc caccgtttat cgccagttgt
     1321 tgaagaagtg ctgtacccgg caatggaaga ctaccaactg gatatcatga ttggtgaagg
     1381 tccggcggca cgctccatta aaattgattt gccgccgttt accctgattg gcgcaaccac
     1441 gcgcgcaggt tcgctgacat caccgttgcg cgaccgtttt ggtattgtgc aacgtctgga
     1501 gttttatcag gtgccggatc tgcaatatat cgttagtcgc agtgcacgct ttatggggct
     1561 tgagatgagt gatgacggcg cgctggaagt tgctcgtcgc gctcgcggta cgccacgtat
     1621 tgccaaccgt ctgctgcgtc gagtgcgtga tttcgccgaa gtgaagcacg atggcaccat
     1681 ctcggcagat atcgctgctc aggcgctgga catgttgaat gtcgatgctg aaggtttcga
     1741 ttatatggac cgcaaattgt tgctggcggt aatcgataag ttctttggtg ggccggtagg
     1801 tctggataac ctggcggcgg ctattggcga agaacgtgaa accattgagg atgtgctgga
     1861 accttatttg attcagcaag gctttttgca gcgtacaccg cgtgggcgta tggcgacgac
     1921 gcgggcgtgg aatcactttg gcataacgcc gccagaaatg ccgtaagtcg gattggtcgc
     1981 tcgcgccgca tccgacaaat gtgttcagcg atagattagc tggcctgctt tttcatcata
     2041 ctgataataa ataacagtgc tgcacatagc accaccgacg gacccgccgg cgtatcgtaa
     2101 aatgcggaaa aggttaaacc gcccgttacg gcaaccatcc ccaccaacac agcgacacct
     2161 gccatctgtt ccggcgtgcg ggcaaagcga cgtgcagtag cagcaggaat aatcagcagt
     2221 gaagtaataa tcaacgcgcc aacaaatttc atcgctacac caatcgtcaa tgccgtcacc
     2281 agcatcaaca acaatttcac gcgctgtaat ttcacaccat caacaaacgc cagatccggg
     2341 ctaatcgtca tcgacagcaa attgcgccat tgccagaaca aaatagccac cacgatgacc
     2401 acgccaatcg caatagcgat gagatcttct ggcgtcactg ccagcaaatc accgaacagg
     2461 taagccatca aatcaacacg aatgttagac atcagactaa cgaccaccag gcccagcgac
     2521 agggcactgt gcgccataat ccctaataac gtgtcaagcg ccagctgtgg acgtttctcc
     2581 agccatacca gaccgcccgc caggagcagc gtaacggcaa ttaccgcata gaatggattc
     2641 acgtccagca acaaaccaaa cgcgacgcca agtaatgagg catgagccag cgtatcaccg
     2701 aaataagaca tacgacgcca gactacaaac gaacccagcg gacccgcggc acaggcgagc
     2761 atgatcccgg ctaaccaacc gggaaataat aattcaatca tgagcgatca tttccccgac
     2821 gcaaaacaat tcgtccctgt aaatcgtgac gatgattatg atgatggcga tagataccca
     2881 gttgttcagc accacgagga ccaaacatag aaataaactc cggatgtagg gaaacaactt
     2941 ccggtgtgcc ggaacaacaa atgtggtgat tcagacacag cacttcatcg gtttttgcca
     3001 ttaccagatg cagatcgtga gagaccatta aaacgccaca atccagttcg cgacgcaact
     3061 ggtcaataag gtcatataac gccacctggc catttacatc cacgccttga gtgggttcat
     3121 ccagcactaa taattgcggt cgatttaaca atgctcgcgc taacagtaca cgctgtgttt
     3181 cgccaccaga gagcttttgc atcggtgcgt taatcagatg cccggcctgg acacgtttca
     3241 gtgcaggcaa aatatcttct ttatgtgtgc cagggcgtaa gcgtaaaaaa cggtttacgg
     3301 tcagtggcaa cgtggtgtcg agatacagct tctgcggtac atagccgatg cgtagttttc
     3361 cgttgcgctt gataacccct tcatcgggtg ttaccagccc gagcactacc cgtaccagtg
     3421 tcgacttacc tgcgccattt ggcccaagta aagtcaaaat ttttccaggt ttaagttcca
     3481 gcgacacatc agagaggacg cggcgttggc caaaagaaac tgagacattt tccagggaaa
     3541 ccagacttgt catgttaatt ttagtcttgc agtagtcatg agatgttata atatcacact
     3601 tctcatattc attacgatga ttggtcgcat tatgttacat aaaaaaacgc ttcttttcgc
     3661 agcattatcc gccgctctct ggggaggtgc aacacaggcc gcagatgccg ccgttgtcgc
     3721 ttcgcttaaa cccgttgggt tcatcgcttc tgccattgct gatggtgtaa cagaaacgga
     3781 ggttttactg cctgacggcg cttcagaaca tgattattca ctgcgtccat cggatgtaaa
     3841 acgcttacag aacgcggact tagtcgtttg ggttggcccg gagatggaag cgtttatgca
     3901 aaaaccggta agcaaattac taggagcgaa gcaggtaacg attgcgcagc ttgaagatgt
     3961 gaaactgcta ctgatgaaaa gtattcacgg cgatgatgat gatcacgacc acgcggaaaa
     4021 aagtgaagaa gatcaccatc acggcgattt caacatgcat ctttggcttt ccccagagat
     4081 agcgcgggct acagcggttg caatccatgg aaaattagtg gaacttatgc cgcaaagtcg
     4141 agccaaactt gacgccaacc tgaaggattt tgaggcacaa ttagcctcaa ccgaaacgca
     4201 ggttggtaac gagctcgcgc cgctcaaggg gaaaggttat ttcgtttttc acgatgctta
     4261 cggctatttc gaaaaacagt tcggactgac accgcttggt cattttaccg ttaaccctga
     4321 gattcaacct ggcgcgcagc gtttacatga aataagaaca cagttggttg agcaaaaagc
     4381 aacctgcgtt tttgctgagc cacagttcag gccagcggtc gttgagagcg tcgcacgagg
     4441 gacatccgtt cgtatgggaa cgttggatcc cttggggacg aatatcaaac tgggtaaaac
     4501 aagctattca gaattcctga gtcaattagc caaccagtat gcgagctgcc tgaaaggaga
     4561 ttaatgagga agtgattacg tgcaacagat agcccgctct gtcgccctgg cgtttaataa
     4621 tttaccgcga ccacaccgcg ttatgttggg gtcgctcacc gttcttactc tggccgtcgc
     4681 tgtctggcgg ccctatgttt atcaccgcga tgccacgcca attgtcaaaa ccattgagct
     4741 ggaacagaac gaaattcgtt cgctcttacc tgaagccagt gagccgattg atcaagctgc
     4801 acaagaagat gaagccattc cccaggacga actggatgac aaaatcgccg gtgaagcggg
     4861 cgtgcatgaa tatgttgttt ccactggcga tacgctaagc agcattctca atcagtatgg
     4921 tattgatatg ggtgatatca cccaactggc tgcggccgac aaagaattgc gtaacctgaa
     4981 aatcggtcaa caactctcct ggacattaac cgcggacggc gaactgcagc gcctcacctg
     5041 ggaagtgtct cgtcgtgaaa cccgaaccta tgaccgtact gccgctaacg gttttaaaat
     5101 gaccagcgaa atgcagcaag gagagtgggt taacaatctg ctgaaaggta ccgtcggagg
     5161 gagctttgtt gccagcgcca gaaacgccgg tttaaccagc gccgaagtga gcgcagtgat
     5221 taaagccatg cagtggcaaa tggatttccg caaactgaaa aaaggcgatg aatttgcggt
     5281 gttaatgtct cgagagatgc ttgatggtaa acgtgagcaa agccagctgc tgggcgtacg
     5341 tttgcgttca gaaggtaaag attattacgc aatccgcgct gaggatggca aattctacga
     5401 ccgtaacggt actggtctgg cgaaaggatt cttgcgattc ccgacggcga aacagttccg
     5461 tatctcgtct aactttaacc cgcgtcgtac taatccggtg accggtcgcg ttgcaccaca
     5521 cagaggtgtt gatttcgcca tgccgcaggg tacgccagtg ctttcagtgg gtgacggtga
     5581 agtggtggtt gccaaacgca gtggcgcagc aggttattat gtggctattc gtcatggtcg
     5641 cagctacacc acgcgttata tgcacttgcg caagattctg gtgaaaccgg gacagaaggt
     5701 gaaacgtggc gaccgtatcg cgctttccgg taataccgga cgttcaaccg ggccgcatct
     5761 gcactatgaa gtatggataa accagcaggc cgtaaacccg ctgacggcaa aactgccgcg
     5821 taccgaaggg ctgaccggct ccgatcgtcg cgaattcctg gcgcaggcca aagagattgt
     5881 gccgcagcta cggtttgatt aattaacatc cattcgcagc cggtacgcag tcagtaccgg
     5941 ctttttttat ttggtgcggg gcaagttgcg ccgctacact atcaccagat tgatttttgc
     6001 cttatccgaa actggaaaag catggaaacg aaaaaaaata atagcgaata cattcctgag
     6061 tttgataaat cctttcgcca cccgcgctac tggggagcat ggctgggcgt agcagcgatg
     6121 gtgggtatcg ctttaacgcc gccaaagttc cgtgatccca ttctggcacg gctgggacgt
     6181 tttgccggac gactgggaaa aagctcacgc cgtcgtgcgt taatcaatct gtcgctctgc
     6241 tttccagaac gtagtgaagc tgaacgcgaa gcgattgttg atgagatgtt tgccaccgcg
     6301 ccgcaagcga tggcaatgat ggctgagttg gcaatacgcg gaccggagaa aattcagccg
     6361 cgcgttgact ggcaagggct gcagatcatc gaagagatga ggcgtaataa cgagaaagtt
     6421 atctttctgg tgccgcacgg ttgggccgtc gatattcctg ccatgctgat ggcctcgcaa
     6481 gggcagaaaa tggcagcgat gttccataat cagggcaacc cggtttttga ttatgtctgg
     6541 aacacggtgc gtcgtcgctt tggcggtcgt ctgcatgcga gaaatgacgg tattaaacca
     6601 ttcatccagt cggtacgtca ggggtactgg ggatattatt tacccgatca ggatcatggc
     6661 ccagagcaca gcgaatttgt ggatttcttt gccacctata aagcgacgtt gcccgcgatt
     6721 ggtcgtttga tgaaagtgtg ccgtgcgcgc gttgtaccgc tgtttccgat ttatgatggc
     6781 aagacgcatc gtctgacgat tcaggtgcgc ccaccgatgg atgatctgtt agaggcggat
     6841 gatcatacga ttgcgcggcg gatgaatgaa gaagtcgaga tttttgttgg tccgcgacca
     6901 gaacaataca cctggatact aaaattgctg aaaactcgca gaccgggcga aatccaaccg
     6961 tataagcgta aagatcttta tcctatcaaa taaaaaaaag cctctcaaat tattgagagg
     7021 ctgtactcga taaaaaatct caatgagtgt tcccactatt ttgagttttt aggttagtac
     7081 gctattgagt taatcgtagc gtgataattt caaaaggttt gaactcaaga ttgcaatatt
     7141 gattgcaata aaaactgtca tctaacttat tttcttgaag atcgacatat tgaacgtagt
     7201 gataattaca tacccacgaa agcatggctg aaatttctaa tccatgactc tcatataatc
     7261 ttacgattat accgtcgccg tcttcagcta attttattgt ttcgatgata acgctgttag
     7321 tatcgatagc aaaaaatgat agtgaagggg gtaattcacc cgggtgcggt ttgacaggga
     7381 cgacctgtaa gggatgattc attatatagg cttgattaat aacaccaccc acacggtaat
     7441 ctccatgatg aggaaataag gagaatatga atttttgttt accaatatct gcaaaattat
     7501 taatcaggaa attatcataa ttgtctgtct taacaatttc cccaggttta tgctgcgaac
     7561 gcagcaaagt taattcaagc gtctggtctt tgacacgata gccatatttg ttttttgcta
     7621 ttaaagctat gccagtttct cgatcagaaa ggtcgaccca gtgatgtgct ggtatttcat
     7681 ctttggcaaa agcgaacgaa tcatcgctat gcgttggtct gtcaattaca ccgaactgaa
     7741 tttgacattg tgcaagagat gtttgtactg acaccgggaa tttgactttg agagaaacat
     7801 taggttcgtg ccaatcaata aaagtttgga catcaacgcg tggactgtcc tgtgtcaggg
     7861 taaatgcgaa gcgaagataa gaggaattgt agtgataaat cagcgtcatc gtagttttag
     7921 gcccgctgat tacagttgtc attttttcag cgagtaaaac atatgattcc ccatcacgat
     7981 aatttgaagc aaaatcccag gccgcgaaaa aacctgcatc ttcgtggtat gcacggatct
     8041 cactgtgcat attttcagta atgtattctt taccatatct tttatcatat agcgaaatta
     8101 aatcaccgtt ttctgaaaaa agaagtttaa tgtaattatt ttcaatactt cgagat
//
LOCUS       DAZPEB010000175         8148 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZPEB010000175 DAZPEB010000000
VERSION     DAZPEB010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8148
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.178"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..262
                     /locus_tag="ACHURJ_003409"
     CDS             <1..262
                     /locus_tag="ACHURJ_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFR1282222.1"
                     /translation="AVVLGMIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFR
                     FYRLNGDTLRRIQIHLLDKYRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(401..1030)
                     /locus_tag="ACHURJ_003410"
     CDS             complement(401..1030)
                     /locus_tag="ACHURJ_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFR1282223.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATARRYPLTPLLYTLIFLHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(1153..2799)
                     /gene="fumB"
                     /locus_tag="ACHURJ_003411"
     CDS             complement(1153..2799)
                     /gene="fumB"
                     /locus_tag="ACHURJ_003411"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFR1282224.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGIAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDTCHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(2877..4217)
                     /gene="dcuB"
                     /locus_tag="ACHURJ_003412"
     CDS             complement(2877..4217)
                     /gene="dcuB"
                     /locus_tag="ACHURJ_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFR1282225.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(4788..5507)
                     /gene="dcuR"
                     /locus_tag="ACHURJ_003413"
     CDS             complement(4788..5507)
                     /gene="dcuR"
                     /locus_tag="ACHURJ_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFR1282226.1"
                     /translation="MSNVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(5504..7135)
                     /locus_tag="ACHURJ_003414"
     CDS             complement(5504..7135)
                     /locus_tag="ACHURJ_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFR1282227.1"
                     /translation="MRHSLPYRMLLKRPMKLSTTVILMVSAVQFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVVSGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYSLKTANNYKEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESLLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            7316..7546
                     /gene="yjdI"
                     /locus_tag="ACHURJ_003415"
     CDS             7316..7546
                     /gene="yjdI"
                     /locus_tag="ACHURJ_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFR1282228.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVVTVVKVIDTCPSGALKYRHK"
     gene            7558..7830
                     /locus_tag="ACHURJ_003416"
     CDS             7558..7830
                     /locus_tag="ACHURJ_003416"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1282229.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            8057..>8148
                     /gene="ghoS"
                     /locus_tag="ACHURJ_003417"
     CDS             8057..>8148
                     /gene="ghoS"
                     /locus_tag="ACHURJ_003417"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFR1282230.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSL"
BASE COUNT         2021 a         1969 c         2085 g         2073 t
ORIGIN      
        1 tgcggttgtg ttagggatga ttggctatgt accgaatgtt gaacagtcta cgcaagccct
       61 attaggtatg cagtttatta tgattgctct accaactctg tttttcatgg taacgctgat
      121 tctctacttc cgtttctatc gcctcaatgg tgacacgctg cgcaggatcc agatccatct
      181 gctggataaa tatcgcaaag taccgcccga gcctgttcat gctgatattc cggtcggtgc
      241 agtgagtgat gtgaaagcct gacgtggaca atagctaacg aaaatggcct gatgcgatat
      301 gtttatcagg ccacgtctta atgtaatgct ttggatttgc aaattttgta gaccggataa
      361 ggaattcacg ctgcatccgg catcaacaaa gcgcacgttg ttatccggtt atcaagccaa
      421 agcgccgtag ctggcggcaa tgaaagcgag cgaggaatat caccgtcgtt aatgcgccaa
      481 gcagcgcgca gaacatatcg gattgcgtgt cccactggtc gccctgggtg ccgagaaaat
      541 catccgctcc ctgacccatt gccagcgccg cccaccactc gattaattca tacattgcgc
      601 ttatcgccag cgccacgcag cagactagga acgccaccat tttacgtccg cgcacgtaca
      661 tcccgcgaac gagaatttct cgtgccacca gtgcaggcac cagcccctgg aaaaagtgcc
      721 ccagcttgtc atacggatta cggctcaacc ccagccattc ctgcacctca aaaccaacgg
      781 ggactttcgc gtaggtgtat tgtccgccga ccatcaggat gatggcgtga aggaaaatga
      841 gcgtatagag cagcggcgtt aacggataac gtctggcggt ggcaagcagt agcggcacga
      901 caataatcac cggtgtcact tccatcagcc aggtgagttt gtcatgggcc gaaatgccgg
      961 tataaatcag gattaatgtt agcgccagtg cgccggtgtt aagaattaac ggcttgagtg
     1021 tgcgggtcat ggtgagttcg caaatcaggg aaaatactga ctattcacca gcaagcgtga
     1081 aattacaatc gcaaaagagg tggcccaggg ggatcacctg gcagcatgct gccaggcgct
     1141 gggccgaaga ggttacttag tgcagttcgc gcactgtttg ttgacgattt gctggaagaa
     1201 gtcgttacct ttgtcatcga ccaagataaa cgccgggaaa tcttctactt cgattttcca
     1261 gatagcttcc atacccagct ccggataagc gacgcattcc agatgcttga tgctctgctg
     1321 cgccagtacc gccgccggac cgccgatgct accgaggtag aagccgccgt gtttatgaca
     1381 cgtgtcggta acctgctgac tgcggttacc tttcgccagc atgatcatgc tgccgccgtg
     1441 ggattgcagc agatccacgt aggagtccat acggcctgcg gtggttgggc caagtgaacc
     1501 tgatggataa ccggcagggg ttttcgccgg acccgcgtag tagatcgggt gatctttgat
     1561 gtactgcgga agttctttac cggcgtcaat cagctctttc agcttggcgt gtgcaatatc
     1621 tcggcccacg ataatggtgc cggtgagcga caaacgagtg gataccgggt attgcgaaag
     1681 ctgggcgagg atctctttca tcgggcggtt aaggtcaact ttcaccgctt cgccttcacc
     1741 ggcctggcgc agttcttgtg gaatgtactg gcctgggttg tgttccagtt tttcgatcca
     1801 gataccttcg cggttgattt tcgctttaat gttacggtca gcggagcagg agacgcccat
     1861 gccgaccggg caggatgcgc cgtgacgtgg cagacggata acgcgaatgt cgtgcgcgaa
     1921 gtatttaccg ccaaactgtg cgccaagacc gagtttctgg gcctcttcca gcagttcctg
     1981 ttccagctgg acatcgcgga acgcctgacc atgttcgttc ccttccgtcg gcagttcatc
     2041 gtaatagtga gcgcttgcta acttgacggt tttcaggttg gtttccgcag acgtaccgcc
     2101 aatcacaaac gcgatatggt acggcgggca ggctgcagta ccgagggtac gcattttctc
     2161 gacgaggaag tttttcagtt tgccgggagt cagcagggct ttggtttcct ggtagagata
     2221 cgttttgttg gcagagccgc cgcctttcgc aacgcaaagg aatttgtact catcgccatc
     2281 taccgcgtac aggtcgattt gcgcaggcag gttagtgccg gtgttgacct ctttgtacat
     2341 gtccagcgcc gcattctgtg aatagcgcag gttatcttcg atataggtgt tatagacgcc
     2401 tttcgacagc gcttcttcat caccgccgcc ggtccacacg cgctggcctt ttttaccgac
     2461 gatgatcgcg atgccggtat cctggcaggt cggcagcacg cctttggcgg cgatttcgga
     2521 atttcttaag aattgcagcg ccacgtactt gtcgttttcg ctggcttctg gatcgtgaag
     2581 aatagccgca acctgtttct ggtgtgccgg gcggagcata aaagaagcgt cgtgaaaggc
     2641 ttgctgcgcc agcagggtca gggcttctgg ttccactttc aggatggttt cgccgtcgaa
     2701 gtcggcaacg ctaacgtaat cggaagtgag tagatagtat tcggtattgt ctttccccat
     2761 cgggaaaggt gcctggtaga taaagggttt gtttgacata gcttccagcc tgtaactctg
     2821 tatttgttat tcgaaaatgg cgtgccgcgt agtcacgggc ggcacgcaaa gtgcatttat
     2881 aagaacccgt acatcgcggc gaagatccag ccgaagacgc acgatacgct cacaccaatc
     2941 aaccccggca gaataaagct gtggttgatg acgaagcgac cgatgtgggt ggtgccggaa
     3001 cggtcaaact gaatcgctgc cagatcgctc ggataagtcg gcaggatgta ataaccgtag
     3061 caagccggtg ctgaagccac gatgtatgcc ggatcaacgc cgatcgccaa cgcgaccgga
     3121 acaatcgccg ccagcgccgc agcctgagag tttacaaact tggaaaccag cagcagaaca
     3181 atggcatagg cccacggata ctctttcacc atttcaccca gtacgccctg aatttcagac
     3241 atatgcgcac cgaacatggt ttctgccatc catgcgatac cgtacaccgc cacgatggcg
     3301 atcataccgg aacggaagac ttcgtttttt gagatagacg cgggattggt tttggtcagg
     3361 ataataatca gcgccccggt cagcagcata aacatctgaa taaccagtac catcgacagc
     3421 ggtttgccgc cgaaggatgg acgcaggtcc gaatcagcac caagaagggc gactacagcg
     3481 attgccccga ggaaaatcca cattgccagc cagttgcttt tcggcagttt tttatccagc
     3541 agcgtcgcgg tatcaccgta aacatactca cggttttccg gtacggagat gaatttctgg
     3601 aactcttcgt ctttatccag atctttacca cggaaccagc tgaagatacc gatcgccagg
     3661 ataccgatta acgtcgatgg aatggtgatt gccagcaggt cgaggaactc aagatggcga
     3721 ccatcaaagg tgacattacc cagcatcgca accagagaca cgaccgcaac cgacaccgga
     3781 ctggcgataa tccccatctg tgcaccgata gaacttgccg ccatcggacg ttccggacgg
     3841 atgttgttct taatggcgac gtcgtagatg atcggcagaa tggtgtaaac cacatgaccc
     3901 gtaccgcaaa gaatggtcag cgtacaggtc acaaacggcg cgacaattga gacatatttc
     3961 gggttgcggc gcagcaactt ctcggcaatt tgcagcatga catcaagacc gcccgaagct
     4021 tgcaaggtcg ccgatgccgc caccaccgca atgataacca gcatgacatc aaccggtggt
     4081 ttacctggct gaaggtggaa gacgaagacc agaatgacca gaccgatacc gcctaataaa
     4141 cccagcgcga taccaccctt tctggcacca taaaacagac atatcagtat tatgataagt
     4201 tggatagtaa ataacatgtg tgaaccctcg cgataatcct atttaaattt ttgctgaata
     4261 gatcacagtc acgttctgtt ttgtatgaac tgttttcaga gcctgactaa atatccgttt
     4321 ggtcgttacc ggctttagca aatacctcac agtaaatatt ggctgattaa agcagcggca
     4381 aatttatgca ctgtgataag cggctttttc agagagaaaa actctctggc gatgggttta
     4441 tagtttgtac tgcgcagatg ttaatgtttt gtttgctgtt atcttattga tatttatcgg
     4501 tctatttttt gttctcaggg cagatatcat taggtttaaa gattaatctt atctacccat
     4561 ttgtgggtga aaatacacat aatgcgggtg acataatagt taattaactt ttgttagcgt
     4621 tttgaaatta aaaacaccgt tcacctgaag agatattaat ttttagcgat gatggaggga
     4681 taattatatt tgatctggca caagttttac tgatgaagga tgtaacttgt gccaggggta
     4741 ttttgcatta cggtaataat tattacttac agataaccag cgatccgtta ttggcaatat
     4801 tgtttcagta gtgagtagtg ttctgcctga atacggtaac ggtaaaccgg acgccccgtg
     4861 acgccataat ggatactggt gaacaagatg tggcagttga ccagccagat gaggtattta
     4921 cggcaggaaa cacgcgagat gttaacctcg ttagccagct cgtcggttga aaactcatag
     4981 tcctgatgcg cgtcaatcca ctggcacagc gtgcgtaacg tctgcggcgt taagcctttt
     5041 ggcaagcgac gaggatcctg ttcgttggag ctgctgccgt ggattagctg atcaagctcg
     5101 gcctggtcat aatactgatg tttttccagc gccattttct tttgccgcca gccggtgagc
     5161 gcctcttcaa agcgggaagc ctggaagggt ttgatcaggt aatccacgac accgtaatgc
     5221 agcgaatctt taatggttgc cgcatcggct gcggaggaga tgacaatcac atcacttttg
     5281 caacgcgcgt tatgcaggac aggcagtaaa tcgagcccgt tctctttttg catatagata
     5341 tcgagcaata tcaggtcgat aggcgtatcg ctattgaaga taatctcttt ggctttctcc
     5401 agcgtcgagg ctgttccaca gcattgaaag cctgggattt gtgctacgta tcggcgattc
     5461 agctccgcga ccattgcgtc gtcatcgata attaatacat tgctcatctg tttgacctct
     5521 ccccgtccca gggtatctgg acaaaaaatt gtgtgaaaat cccgggttcc gattccacgg
     5581 cgatgctgcc gccgagattt tctacctgtt gtttgacaag tgctaaaccg acgcctcgct
     5641 cgcttccttt tgtcgagaca cctttgtcaa aaatgtgatc gattttgtcg ggtgcgatcc
     5701 ccggtccatc atcgttaact tcgcagtgca gccagccgtg acggtagtgc aatgttacgc
     5761 taatttcgcc accgggttcc ggccctaatg cttccagcgc gttttctatc agatttccca
     5821 atgtggtaat cagcgtcgcg acctggtcct cactgccgct gtctggcagc aggctttcac
     5881 tgtttaaaat cagcgtatgg cctaaatcgg tcgcgcggtt aatcttgctg attaaaaaac
     5941 cagcgataac cggagatttg atcttaccca gcagagagcc aatctcttcc ttatagttat
     6001 tggctgtttt gagactgtaa tcttccaact gcttataact cttcagatgc aataatccga
     6061 gaatcacatg caatttattc ataaattcgt gggatcgttc acgaagtgca tcagcatagt
     6121 tgaccagacc gtcgagtcgc tgcatcagtt tacgaacttc agttttgtcc ctgaaggttg
     6181 aaatggcacc gatgataacg ccattactgc gcaccggaac ggtgttgatc agtaatagcc
     6241 ggtctttaat cgtaatctct tcgtcgcggc gcggcgtacc gtcgcgtaac acttccgaga
     6301 catctaccac ctgtgaccat gagtggctta gcgtcgacag tttctcatcg tcctgcgact
     6361 tacggtaatt cagcaattct tgtgcggcat cgttgatcag cgtgacctcg ccgcgatcgt
     6421 ccacggcaac gacgccttct ttgatagact gcaacatggc ctggcgctgc tcaaacagcg
     6481 tggagatttc gtagggttcc aggccgaaaa ggattttttt cagtacctta accagaatgc
     6541 aggtgccaat cagtccgacc agcatgccaa ataataccga ccagataatg ctccagcgac
     6601 tgtcattgat ttgttgggtc acgcggctta actcaaggcc gctcaccacc acgccaattt
     6661 gtttatgatt ttcatcgtag atgggggtaa atacgcgtaa agcctgcgcc agaaaaccgc
     6721 gattgatagc gacattttct ttgccattca gcgctttaag gatgtcatca cctttaaatg
     6781 gctgaccaat acgctgggct tcaggatgcg agtagcgaag actttgcata tccgtaacga
     6841 caataaacag cagatcgttg cgtttgcgca cggcttccgc gatggcctgg attccgctct
     6901 cctgcggttt tttctgcaag ccctgacgga tttccggcga gtcggcgagg gtacgcgcca
     6961 ctgccagtgc cttgttggcc agcccgtctc gtgtcatatc actgatttgc gagaagtaaa
     7021 tcagatgcac caccaatagc accgagaact gtaccgcgct gaccattaag atcactgtgg
     7081 tactcaattt catcggacgt ttgagtaaca tgcggtaggg caatgaatgt ctcatcagct
     7141 tccttgtgtg acaaatttct taagcattat ctctgatgag gcgggtaatt caaagggcgt
     7201 aagaatgatt ggctatatag gggaagagac tctggcaacg gaaactgcca gtgctgtatg
     7261 aagattccag ggctatgctt atagcgataa tcatactgat gagagaggga aagtcatgga
     7321 tcaggcgcta ctggacgagg gttatcgctg ttataccggc gaaaagatcg atgtctattt
     7381 caacactgcg atatgtcagc attctggcaa ttgcgtacgt ggcaacggca agttatttaa
     7441 tctcaaacga aagccgtgga tcatgccgga tgaagtcgac gtcgtcactg tggttaaagt
     7501 gattgatacg tgcccgagcg gcgcgctgaa ataccgtcat aaataagcga gggtaaaatg
     7561 gaaatacgcg aaggccacaa taaattttac attaatgacg aacagggcaa gcaaatcgct
     7621 gaaattgtct ttgtgccgac cggagagaat ttagcgatta tcgaacatac cgatgtcgat
     7681 gaaagcctga aagggcaggg gattggtaaa cagctggttg cgaaagtcgt ggaaaaaatg
     7741 cgtcgggaaa aacgaaaaat tatcccacta tgcccatttg cgaaacacga atttgataaa
     7801 acgcgggagt atgatgatat tcgcagttga tgggagagta cagagtcacg atatttttca
     7861 tgctctccgc ggagtgatgc aggagagcat ctgaagggta gggggatgca caaagaatgg
     7921 gcagagagcg cgtttttttg tcccacgtca tcccctttac tgagcaaaaa aaagaatatc
     7981 tcctatatga gaatcatcaa tcggggttaa taagttttgc gtccccagag cattcaatat
     8041 tgataggagt catattatgg aaggtaaaaa caagttcaat acttatgttg tttcttttga
     8101 ttatccatca tcttattcct cagtgttctt aaggttaaga tcattgat
//
LOCUS       DAZPEB010000176         7818 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZPEB010000176 DAZPEB010000000
VERSION     DAZPEB010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7818
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.179"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            268..411
                     /locus_tag="ACHURJ_003418"
     CDS             268..411
                     /locus_tag="ACHURJ_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFR1282231.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            424..633
                     /gene="cspE"
                     /locus_tag="ACHURJ_003419"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             424..633
                     /gene="cspE"
                     /locus_tag="ACHURJ_003419"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFR1282232.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            799..1608
                     /gene="rlmA"
                     /locus_tag="ACHURJ_003420"
     CDS             799..1608
                     /gene="rlmA"
                     /locus_tag="ACHURJ_003420"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFR1282233.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDDKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1605..2171)
                     /gene="mntP"
                     /locus_tag="ACHURJ_003421"
     CDS             complement(1605..2171)
                     /gene="mntP"
                     /locus_tag="ACHURJ_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFR1282234.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(2599..3057)
                     /locus_tag="ACHURJ_003422"
     CDS             complement(2599..3057)
                     /locus_tag="ACHURJ_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFR1282235.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDNLERIYKLIVLTQ"
     gene            complement(3112..3963)
                     /gene="manZ"
                     /locus_tag="ACHURJ_003423"
     CDS             complement(3112..3963)
                     /gene="manZ"
                     /locus_tag="ACHURJ_003423"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFR1282236.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(3976..4776)
                     /gene="manY"
                     /locus_tag="ACHURJ_003424"
     CDS             complement(3976..4776)
                     /gene="manY"
                     /locus_tag="ACHURJ_003424"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFR1282237.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(4839..5810)
                     /gene="manX"
                     /locus_tag="ACHURJ_003425"
     CDS             complement(4839..5810)
                     /gene="manX"
                     /locus_tag="ACHURJ_003425"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFR1282238.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            6127..6285
                     /gene="yoaL"
                     /locus_tag="ACHURJ_003426"
     CDS             6127..6285
                     /gene="yoaL"
                     /locus_tag="ACHURJ_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFR1282239.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            6273..>7818
                     /gene="yoaE"
                     /locus_tag="ACHURJ_003427"
     CDS             6273..>7818
                     /gene="yoaE"
                     /locus_tag="ACHURJ_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFR1282240.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQQG
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIVKE
                     QPAHDED"
BASE COUNT         1986 a         1911 c         1942 g         1979 t
ORIGIN      
        1 gcactatgta ataacttctc ccactggcct ggaacaactg aacttattga actatgttag
       61 aaaatacgcc agtttaagta tctgcctgaa ctggcaaggt taagcacaat gatatatcgg
      121 cgcgtattcc gttgcataag tgtgcaaaaa aagtggaaga cgtatcgaga tttgtgcgtc
      181 tgatcgagac atgtttaaaa atggcttgcc ataattaacg ttgtatgtga taacagattt
      241 cgggttaaac gaggtacagt tctgtttatg tgtggcattt tcagtaaaga agtcctgagt
      301 aaacacgttg atgttgaata ccgcttctct gccgagcctt atattggtgc ctcatgcagt
      361 aatgtgtcag ttttatctat gttatgcctg cgggcgaaga aaacaatcta aggaattttt
      421 caaatggcaa agattaaagg tcaggttaag tggttcaacg agtctaaagg ttttggcttc
      481 attactccgg ctgatggcag caaagacgtg ttcgtacact tctccgctat ccagggtaat
      541 ggcttcaaaa ctctggctga aggtcagaac gttgagttcg aaattcagga cggccagaaa
      601 ggtccggcag ctgttaacgt aacagctatc tgatcgaatc cactgatctg aagtgtgaat
      661 acgcttcaat ctcgctataa agcctcgtcg aatgcgaggc tttttactat gctttatctt
      721 cgctcctggc gttcggatat ttgcccgccg cgtgattcgc gttacacttg cggcctttag
      781 tatcctgccg gagttgtcat gtctttttcc tgtccacttt gccatcagcc tctttcgcgt
      841 gaaaaaaaca gctatatctg tccccagcga catcagtttg atatggcgaa agaagggtat
      901 gtcaatctgc tgcccgttca gcataaacgg tctcgtgatc cgggcgacag cgcggaaatg
      961 atgcaagcac gccgcgcatt cttagatgcc ggacattatc agccgctgcg tgatgcaatt
     1021 gtcgcccaac tgagggaacg gcttgatgat aaggccacgg cggtgctgga tattggctgt
     1081 ggtgaagggt attacacaca cgcatttgcc gatgcgttgc ccgaaatcac cacgtttggt
     1141 ctggatgttt cgaaggtagc gataaaagcg gcggcgaaac gctatccgca ggtcactttt
     1201 tgtgtcgctt ccagccaccg tttgccgttt tccgatacca gtatggacgc cataatacgt
     1261 atttacgcgc cgtgtaaagc agaagaatta gcacgagtag tgaagcccgg cggctgggtc
     1321 attactgcca cgccgggacc gcgacatttg atggagctga aggggctgat ttacaatgaa
     1381 gtacatcttc atgcacctca tgcagaacaa ctggaaggtt ttacattaca gcagagtgcg
     1441 gagttgtgtt atccgatgcg tcttcgcggt gatgaagctg tcgcattatt gcagatgacg
     1501 ccgtttgcct ggcgtgcgaa gccagaagtc tggcaaacac tggcagcaaa agaagtgttc
     1561 gactgccaga cggactttaa tattcacctc tggcagcgtt cttattaacc gtggaagtgc
     1621 gtccagagga tctggacgcc gatgccgatc agtaccagcc cgccgagaat ttccgctttt
     1681 ttcccaataa ttgagccgat aaagcgacca accatcatcc ctaatgttga cataatcaag
     1741 gttgcacaac caatggccaa tgcggtcgcg ataatgttga cctgcaggaa agcaagacca
     1801 acacccacag ccatggcatc caggctggtg gcaatcgcgg tggttaccag tagccagaaa
     1861 ccgtgtcgac ggcgcggctc ttcatcttca tcatctgcgc cacgaaaacc ctcaataatc
     1921 attcgcccgc cgaggaatat cagcagcaca aacgcaatcc agtggttcca ttcaaggaca
     1981 aaccggctgg ctaacatgcc cattccccag ccgatcagcg gcgtcagggt ttcgacggca
     2041 ccaaaaataa ggccggttcg caatgcttca gaaaatttcg gtttatggag ggtggcacct
     2101 ttaccgattg atgcagcaaa tgcatccatc gacataccaa acgcaagaag aacagtagca
     2161 gtgatattca tgacaatgtc ctgaccgggg tatccataaa acacatcatc gcccccagca
     2221 aacatgcata catgaacaac aagccgtgat ggtttttgcg ttcatgcagg catagcgatt
     2281 gatgtgcata tggtctcgcc tgactgaaag tattcagtcc gtacgtgcca cgttttgcaa
     2341 cgagtatgtt gacacgtaca tttccggaat ctggaaatcg gctactcccc aaatgagggc
     2401 gcaaccttaa catattttga gaatataaaa caactgttgg aatatattat ttcattgctt
     2461 tattgataac gaattcattt agatttatat atcaatgaaa cgttaaaata aataacaaat
     2521 aagggaagag gtaatatagc ctaagctatg tctgattatt aaaaatacac taaacatagc
     2581 tttggctaaa ttcataactt attgagttaa aacgataagc ttataaattc tttccagatt
     2641 gtcgatattt cgaacgcgga ttaacagccg acgctgttct aattgcatca ccaacacgcc
     2701 atcttccgac aagttcatcg ctttgattcg gctatattca atccagacat tggcgaagaa
     2761 aaaacctttt tgtttaaaga tgatcttcgg aacgcggatc cagaatatat aaaaacccat
     2821 cagagccagt gcgcttaata accatgtcgt tattaatgca ccatgattcg tgacgttgtt
     2881 atagataaga atgacaatca atccgacgaa gataacgcta tcgatgcgac cacgccggag
     2941 caaaggaatt gccagcaggg tggggccgtt acggcggggc atgatgaact gatcgtagat
     3001 cgcgaaggcc aggagtgcgg cgatgaaaag aatcagtacc aggtccgtga ttgtcattaa
     3061 tcctccagat aaaaaaacgg ggccaaaagg ccccggtagt gtacaacagt cttacagtcc
     3121 cagcaggccg caagcgtaac cagcgatacc gatgacgaag aagccaacga tgatccacag
     3181 cgggttaact tttttgcgca gtagccacat acaagcaaag gtcagcagca gtggtaccag
     3241 gcctggcatt aactggtcca gaatagtctg gacagtagta acgtgttctt tgcccgtctg
     3301 gtcagtaatg cgagagacaa ccagcgggat gttgacatgt gtccacttgt taaccaatgc
     3361 ccccatgaca aacaggccga ggatagacgc cccttccgtc agtttttgca ggaagccacc
     3421 acccatatct ttaacgatat cgataccttt ggagtaacca tacgctacgc cgtagtaacg
     3481 ggttgccaga cgcaccaggt taaacaggat gaagaacagc agcggaccta acaggctgcc
     3541 gctcatcgcg ataccggcac ccagtgctgc aaataccgga cgtacggttc cccagaagat
     3601 cgggtcgcct acaccagcca gcggccccat caaaccgact ttgataccgt tgatagcacc
     3661 gtcgtcgatc tctgcgccat tagcacgctg ttcttccagc gccagggtta cgccgagaat
     3721 cggcgcagcc acgaacggct gggtgttaaa gaactccagg tgacggcgaa tagcttgttt
     3781 acgagcttcg ttgttctcag ggtagaggcg acgaattgcc ggtaccatag agaagcagaa
     3841 acccagtgcc tgcatacgtt cgaagttcca tgaaccctgg aagaggttag aacgcaggaa
     3901 gacgccacga atatcacttt gagtgagttt tttctcggtg gtagtttgag ttgtatcaac
     3961 catttcgctc acctgttagt ccagttcgtt atcgagatcg ttgttaccag cagcctgagc
     4021 aggcgcaccg gctacgcggt tgtatttcgg gctaagttgg atgtagagca ctgccataac
     4081 agtaccgatc acacccagag caaccaggtt aaagttggtg aatgctgcgg ttacgaagcc
     4141 gaggtagaag aacggcatca ggtagccagc acgcatcatg ttgataacca tcgcataacc
     4201 aaccaccacg atcatgccac cggcgatatt cagaccattg gtcaccactt ccggaatcgc
     4261 attcagcatg ttctgtactt cgctggtacc aacagacagc gcaacgatga cggccggaat
     4321 agccacacgc attgcttgca ggaacagaga agaaacgtgg atccaggaaa tcgctgtcag
     4381 gttgccgtta tcagcagcct tatccgcagc gtgctggaaa gcaacggtaa tagtacgaac
     4441 gatgatggtc agtacctggc ccgcagcggc cagagggatt gccagtgcga tacctgcacc
     4501 aatgctctga tgacctgcga taaccagaat ggtagaaatg atagaagcca gagcggcgtc
     4561 aggcgcaact gcagcaccga tgttcatcca gcccagcgcg atcatttcca gcgtaccacc
     4621 gataataata ccggttttca tatccccaag aacgataccc accagggtac acgcgattag
     4681 cggacggtga aactgaaatt catcgaggat tgatcccata cctgcgatac aggctacgat
     4741 aaataccagc acaatttgaa gagtggtaat ctccattgta cttctcctgt ttacgtaaga
     4801 cttaagtgtg aaaactgagt gataatcaac acaatacgtt acttatcgat tttgctgatc
     4861 agatccatca ttttcagttt cggatcggtg gaaaccttac ggacttccag ctcaataccg
     4921 cgcgcattca gtttcttgaa cgcctcgata tctttttcat caaccgaaac cgcgttattc
     4981 acctgggttt taccctgacg gaatgccata ccaccgacgt taacagaggt gattttcacg
     5041 ccgccttcaa cgagacgctc tacatctgtt gggttggtaa ataacagcat tacgcgttcg
     5101 ccagcatatt tcgggttgtt gtagacgcga atcattttgg caacatcaac tacgtgtgct
     5161 gttacgcccg gaggtgcaac ctgggtgagc agtgtcttac gaacggtatc cgcagccact
     5221 tcatcactaa caacaataat acgggagaca ttggtttctt tggtccagcg ggtggcgacc
     5281 tgaccgtgaa tcagacggtc gtcgatacgc gcaaggccaa taaccatgta gtcgtttggc
     5341 cccattggtt ttgccggagt tggagccgct tttggtgctg cggcagcggg tgctggcgcg
     5401 gctttttcaa ccggtttggc tttcagtgct ttcacgcctt cacggcctgt ttctactgcc
     5461 agcgcaacca gttcatcaaa gcttgggtca tcatcacgag ccattaacgt ttccacgagc
     5521 attggaatgt taacgcctgc aatgacttca taatgctctt tgtcgacgac aatgcggctg
     5581 gcagcattga acgggctgcc tccccatgta tcaacgagaa acagcacgcc tttagtggtg
     5641 tcgagttttg ccaactgagc gttgtacttt tcaatcagcg tttcggcatt ttcacctgga
     5701 acgaaatcga tccagccgac gttttcctgc tcgcctaaca gcatttctgc cgttttaagc
     5761 aactgctctg cagcccaacc atgtgtgcct ataacaatag caatggtcac ttgctacctc
     5821 ctttattatc gttaacacct caacgtgcca gatgtatttt tgaatcgtcg tccacaatcg
     5881 aatcgattca gataagggct acaaccaaaa acccctgcgt ttcgcgattt attttagata
     5941 tcgaaaaaat tattttatgt gatgaagatc cgtaatttaa ctttcgatta gcagaaattt
     6001 cgaaaggtaa aatatccttg tcacattcgt ttgcaaagga aggtaaatct ttgccaaatc
     6061 agaggcgtct ctgatatgtt taactcccgt ttaacaacca tggagtatag ggcagtagcc
     6121 cgcagtatgg atcgtcaccg acgtcatttc agcatcaggc cttttaacgc ctgcctttct
     6181 ggcactctat gccgcacctt tcgtttgcat tttgtcgtta cgcctgcatt atttctggcg
     6241 tcgaatagct attccttaag caggagcttg tcatggaatt cttaatggac ccctcaattt
     6301 gggcggggct actcacgctt gttgttctcg aaattgtgct gggtatcgat aacctggtct
     6361 tcatcgccat tcttgctgac aaactgccgc caaaacaacg cgataaagcg cgtttgctgg
     6421 ggttatcact ggcgctgatt atgcgtctgg ggctgctgtc gctgatttca tggatggtca
     6481 cgctgaccaa accgctattt accgtcatgg atttctcctt ctccggacgc gacctgatta
     6541 tgttgttcgg ggggatattc ttgctgttca aagcaacaac cgaactgcat gaacggctgg
     6601 aaaaccgcga tcatgattcc ggccacggta aaggctacgc cagtttctgg gtggtcgtca
     6661 cacagattgt catccttgac gccgtcttct cgttggatgc ggtaattact gcagtaggga
     6721 tggttaacca tctgccggtg atgatggcgg cggtagtgat tgcgatggcg gttatgttgc
     6781 tggcatccaa accgctgacg cgattcgtta accagcaccc cacggtggtg gtgctctgtc
     6841 tgagcttcct gttaatgatt ggtcttagtc tggtggcaga aggtttcggt ttccacattc
     6901 cgaaaggtta cctgtatgcc gcgattggct tctcgatcat catcgaagtg tttaaccaga
     6961 ttgcgcgtcg caactttatt cgccaccagt cgactttgcc gctgcgagcg cgtactgccg
     7021 atgccatcct gcgtttgatg ggcgggaaac gtcaggccaa tgttcagcac gatgccgata
     7081 acccgatgcc gatcccggaa ggtgcatttg ccgaagaaga acgttacatg attaacggcg
     7141 tactgacgct ggcgtcgcgt tctctgcgcg ggatcatgac gccgcgcggt gaaataagct
     7201 gggttgacgc taatctcggg gtcgatgaaa tccgcgagca actgctctct tcaccgcaca
     7261 gtctgttccc ggtatgtcgc ggtgaactgg atgaaatcat cggtattgta cgtgctaaag
     7321 aactgctggt ggcgctggaa gagggcgttg atgtggcggc gattgcttcg gcgtctccgg
     7381 cgattatcgt cccggaaacc ctcgatccga tcaacctgtt gggcgtgctg cgtcgtgctc
     7441 gcgggagctt tgttatcgtg accaacgagt ttggtgtggt acaaggtctg gtcacgccgc
     7501 tggatgtgct ggaagccatt gcgggtgaat tcccggacgc tgacgaaacg ccggaaatca
     7561 ttactgatgg tgacggctgg ctggtaaaag gcggtacaga tttgcatgcc ttgcagcagg
     7621 ggcttgatgt tgagcacctt gccgatgacg atgatatcgc gacggtcgcg ggcctcgtga
     7681 tctcggcaaa tggtcacatt ccccgtgtgg gcgatgtgat tgatgtaggg ccactgcata
     7741 tcaccatcat tgaagccaat gattatcgtg ttgatctggt tcgcattgtt aaagagcaac
     7801 cggcgcacga tgaagatg
//
LOCUS       DAZPEB010000177         7702 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZPEB010000177 DAZPEB010000000
VERSION     DAZPEB010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7702
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.181"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..787)
                     /gene="fimH"
                     /locus_tag="ACHURJ_003428"
     CDS             complement(<1..787)
                     /gene="fimH"
                     /locus_tag="ACHURJ_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFR1282241.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLG"
     gene            complement(807..1310)
                     /gene="fimG"
                     /locus_tag="ACHURJ_003429"
     CDS             complement(807..1310)
                     /gene="fimG"
                     /locus_tag="ACHURJ_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HFR1282242.1"
                     /translation="MKWRKRGYLLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGETKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(1323..1853)
                     /gene="fimF"
                     /locus_tag="ACHURJ_003430"
     CDS             complement(1323..1853)
                     /gene="fimF"
                     /locus_tag="ACHURJ_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFR1282243.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(1863..4499)
                     /gene="fimD"
                     /locus_tag="ACHURJ_003431"
                     /pseudo
     CDS             complement(1863..4499)
                     /gene="fimD"
                     /locus_tag="ACHURJ_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
     gene            complement(4566..5291)
                     /gene="fimC"
                     /locus_tag="ACHURJ_003432"
     CDS             complement(4566..5291)
                     /gene="fimC"
                     /locus_tag="ACHURJ_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFR1282244.1"
                     /translation="MSNKNANVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWMENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQFAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(5328..5867)
                     /locus_tag="ACHURJ_003433"
     CDS             complement(5328..5867)
                     /locus_tag="ACHURJ_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1282245.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPVNWKRLYSGSTSLHFIAKYRATG
                     RWVTGGIANAQAWFSLTYQ"
     gene            complement(5932..6480)
                     /gene="fimA"
                     /locus_tag="ACHURJ_003434"
     CDS             complement(5932..6480)
                     /gene="fimA"
                     /locus_tag="ACHURJ_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFR1282246.1"
                     /translation="MKIKTLAIVVLSALSLSSTAALAAATTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQIVQLGQVRTASLAQEGATSSAVGFNIQLNDCDTNVASKAAVAFLGTAID
                     AGHTNVLALQSSAAGSATNVGVQILDRTGAALTLDGATFSEQTTLNNGTNTIPFQARY
                     YATGVATPGAANADATFKVQYQ"
     gene            complement(6957..7553)
                     /gene="fimE"
                     /locus_tag="ACHURJ_003435"
     CDS             complement(6957..7553)
                     /gene="fimE"
                     /locus_tag="ACHURJ_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313299.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HFR1282247.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTLERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
BASE COUNT         2005 a         1940 c         1709 g         2048 t
ORIGIN      
        1 ctcctaacga taccgtgtta ttcgctggaa taatcgtacc gttgcgcgtc aactgtacgc
       61 cgacgccctg cgctggtgaa aacgacgcgg tattggtgaa aatcgagttg cccgcatctg
      121 cggttgtgcc ggagaggtaa taccccaggt tttggctttt cgcacaataa acggtaagag
      181 gaattggcac tgaaccaggg tagtccggca gagtaacggt gacatcacga gcagaaacat
      241 cgcagccgcc agtaggcacc accacatcat tattggcgta aatattccac acaaactgga
      301 aatcatcgct gttatagttg ttggtctgtc gcaaaataag cacggcaatt aatgagccag
      361 ctttaatcgc caccccgccc gcactgctca caggcgtcaa ataaagcgcc accggccacg
      421 gcttatccgt tctcgaatta taaacaaccc gcggcgtttc gctggtggtc ggaaatggat
      481 agctactgcc actatatttt acggtcccgg aaaaattaga taacacgccg ccataagccg
      541 agcctcgttg cagtgtgaca tagtctgtaa tggtttccgg ataatcgtta tggcaaaaga
      601 tttgcgtcga aagatctacg accaggtttt gccccacatt cacgacaggc gcaaggttta
      661 cataaacatt agcgctgcca ccgccaatag ggatagcagt accattggcg gttttacagg
      721 cgaatgacca ggcatttacc gaccagccca tcagcagtac agcaaacagg gtaataactc
      781 gtttcattac aattatctct tcgggttcag ctgtaggtat aggtgatgct aatcactgcc
      841 tgaatggttc cctgagtggc tccgccattt accgtcaatg ctctgacctg taacgggaag
      901 tgcgctgatt gtgaggaatc atccacctga actgttttgg tttcgccagt attcaatgtg
      961 ttgccactgt catcctgtag ctctaactgg atgttttgcg cggtcccctg gtttttataa
     1021 tatccggtac tgtcggctgc cccgctgaag ctggcagtga ccctcgacgt tcccaccgga
     1081 caattagtca actcaagcgc aacatcatgc caggccgatg ccgccccggc agacatcaga
     1141 ctgaaagaat aaagatcgcc gagatcaacc gtggcattgg tggtggaaac tgtgcacggt
     1201 ttggcgacga ccttaccgtt caccgtgatg gtgacatcgg ctgcctgtat tgtcgcactt
     1261 gcgagcgcca atattgccgc caataaatac ccacgtttgc gccatttcat gagcatctcc
     1321 agttactgat attcaagagt gaaggtagcc gtggcattga tatgccccgc agtgacaggc
     1381 acctgtgtcg ccattagccg ggcgtaaaaa ttcaacgtat ttggtttacc cggcgtcagg
     1441 gtcgtccacg aaatcgcgga cgatggagca ttaaggggta tctgattttg ctgctcattc
     1501 agaagctgta ttcccagtcc cgaagccgct gacaccgtat tttcaagtgc aagcaggttg
     1561 gcattgtggc tatctgcaac gccggtaaac ccaaccttta cggcagaaac ggcgttacca
     1621 cagggtgaca gcaaaatacg aaatggaacg acaggagtcg ttgcgccaat gttgttaaat
     1681 tgcttcgccg cgttttccat cagatcaaca gtaaaattgg ttgattcagc ggccacacta
     1741 cagccgttat ctctgacata gccgcggata gtaatcgtgc tatccgcagc caaaacgcga
     1801 cttaccgcca gccacaaaaa agcgcacaga agataaaaag gtttgtttct catcacgccc
     1861 ccttaacgac attcagctga tagctgggtt aataactgct gctgactctc tggtggcagt
     1921 tgataattgg cgatacagtg agcattttcc tcttctcccc atttcacctg aacttttccc
     1981 gctaaaggca ttccgctgag gtaaacctga ccattatccg caacaatgcc gctactctgg
     2041 ctactctctg atgtcaccat cgccccaaac ggcagcggct tattattgtg ggttagcgtc
     2101 atgagcagtt ttatcccaac gcgcgcttta aactctgctc gcacgatcgc cccacgagtg
     2161 ggaacaacgt tagcgaccgc gttatctaaa tcgacgttat cagccagggt attggtatcc
     2221 agcgccactc tgttttcccg atattcggtg gcataaggca gcacggcata accgcgccag
     2281 tcggtacgca cccccgtctg gttttcgact tttgcatctt ttgcgccagg cgctttaaca
     2341 agtaccaccg tatcgtttaa cggctgcccc agcgttacgc cattggcatg agccagtacc
     2401 ccaccgctga ctccgtaata gagctgctta atatcatcgc tatggctgta accgatattg
     2461 gcattgccgt aaccaccgcg ataattcagc gtggcgtagc ctgtacttcc gctattacca
     2521 tcgcctcccc cggcatagcc ggtttgcacg ctatagctga ggttgttgtc ttccagcaac
     2581 gtaccgtata caccagccag attggtcatc cgaccgttga gatcgtgtga cacgctgtag
     2641 ctggcactgg catgtcgcca ctgagattta ctgtcagaac gcagccagtg gctgaaagga
     2701 atattgacgt taagcgctaa catctgatcc cgcccttttt gccaggcgtt tttcgtcagg
     2761 ctatagctga gcgtccagtt gatatcttcg aacgcagtat ttaatccagc ctagaattgc
     2821 tcatcgacat tactcgttcc ccaataagtt tgatggctac cactcaaata cagtgttgat
     2881 gtgcgcccga gttgctgagt aacggtgagc tgtaatttcc cgcgtttgtt ataagcgagg
     2941 ttgtaatagt cggtgaattt cggcttaacc tgaataactc catcctgtgt ttcgatgttg
     3001 tagccattca ttcgactgta tgttgtatca gcgaaattaa aatatccgct ggtcgaataa
     3061 cggtaaccca ctaactgaat attcgtgcct gactcattga gcgatttgtt atagagaaaa
     3121 cgcaccgatt gtccgtcatg ctgactgtca tcgggaagtg tggaattagc ctgcgtcata
     3181 tccacagaca gagcgcccag tgcccccatg tttttcccga taccgaaatt aaaagcacga
     3241 taacgatccg ccagttgcgt tccaccatat attgtccagc cagccggaag gccgtggagt
     3301 aatgtacttt ggaaaaagcg gggtttttcc tgctgcgtat ttccactacg gtattctcct
     3361 gccgtaatgg aataacgagt atgcccttca cgttgcaaaa gcgggactga cgaatagggt
     3421 acggtaaaaa tctgcgtgct gccgtcagcc tctttgatcg ttacctgcaa gtcaccacta
     3481 ttacctgcgg catagatatc gttgatggta aaaggccccg gtggcaccgt actattataa
     3541 atgtcatacc cattttgttt aatagtgacc tgtgcagtac cacgagcaat accgtggatc
     3601 accggggcaa atcctctttg gctatcgggt aacatattgt catctgaggc caattgtgcg
     3661 ccgcgaaagt taataccatc gaaaatatcg ccctgggtat aaccatcacc cagcgtcagc
     3721 cgggaacgta acggtattat gtctcgctca agccaggtat tgatatgctg ccatttattt
     3781 ttgctacctg atgatctgtc gctactgtta taactccagc tggtattgtc gcgtaaacac
     3841 cacgcaccaa tatttaaccc actctgtagg tttaaatatg cataatggct gttaccccca
     3901 atctgattct gtacactatt tccgctgaaa ttataattga gcaatccggc attaataccg
     3961 ggatcccata actcaggagg aatataacca cgcgcgcgat tactcataaa tgcctgaggg
     4021 atcgtcaggt tcagtcgctg ctgaccaaca tccagatgcg cagtagcgtc atggatcatt
     4081 gaggttaatg gcacgcaggc atcatctgcc agcagattca taccggagac agaagccgca
     4141 ttcagcccca tactggcaag ttgcgcgcgt gtcaggcagg gaacaatccc ttgttcactg
     4201 tcgcccgtat taaatgtgac atcacgcgtt gccatataac cattattcaa atagatatcg
     4261 acgcgatacg tccctggcgg taattcttgc ccattttcaa aacgcgataa atcggccaca
     4321 gcctggggat catccgctaa aaagcgcgga ttaaaataga gttcggcaga tgacaaaggt
     4381 gcctgtgcgg caaaagcaca ggcgacaacg agccggacaa aaaaaccagc caaacgatgc
     4441 ttacgaatat gcaagcattg tgtgtttcgc tggtaaagtc ttaaattcag atatgacatt
     4501 attcctccgg tttaaaatta cccggcagtc aattcttttt attcaggcga gaaattcccc
     4561 ctgcgttatt ccattacgcc cgtcattttg ggggtaagtg cgccataatc atttattgtt
     4621 cggtaagtaa tattgcttcc tgcatcagaa ggcaatttaa ccgtgctttc gcccattgga
     4681 ggcaccaatg cattttcaag aacccgggtt ccggcattca actctgttac cgtcagataa
     4741 tagggtgtcg ggttaatcag cgtcagagaa ttcgcgctac gacgaaatct taatttttct
     4801 gcggcctgat cgggtggcaa cgctaattta gccgggcgat agtacagttt aatgcggctg
     4861 ataattgcga actgtagcgt attctcagtc aattttgatt tatccattga cggaatcgct
     4921 ttaacattca tccagaataa actttcccgg tcctgtggca attggttatt tgttgcatca
     4981 agaatacgca aggtattctc tttttttccc ttcatcgcaa acagaggagg cgtcacgata
     5041 aaacgaccat cctttacacc atcggcattt tccatccatg attgaattaa ataggtacta
     5101 ttttcatcat tatttgtcac ggcaagttgc acttgttttt gccctgccgg ataaattacg
     5161 cgagtcgcac ctaaggccac tcccgcttca gcgcgtccgg caaccatcat tgccatgaac
     5221 atcaggatac ctgccagcaa gcagaatgtt atttcctgcg atttccttac attggcgttt
     5281 ttattactca ctgtcctgtt cctgttatca cattatctgc tgaacaatta ctgataggtt
     5341 aaagagaacc aggcctgggc attggcgatg ccgccagtaa cccaacgccc ggtagcacga
     5401 tatttggcga tgaaatgtag cgaagtagag cctgagtaaa gccgtttcca gtttactgga
     5461 ggacgattaa tcggtacgag gtttccttca tcatcaaaca acgctacgcc aatattggtg
     5521 gctatccctg gcccctctcc cacggaaagc acatccggat ttttaccatc cgcgacaccg
     5581 tgaaacgcca cacccacacg ttcactcacc accgtgctac attcccgtaa atgaataaca
     5641 aaaggcaccg gtgcgctatc ttcccctacc gcatgaaacc ggttactgct gatttgcccc
     5701 atattgaccg tcatttgttt atcaccggct tcaatccggc aagtttccgc aataatgacg
     5761 ccctgaaatt gcatatttcc gccgggcagc gtagtattcc atttatttcc ggccagagca
     5821 aacattggca gcaacgacgc taatagaaat aatctcttcc ttttcatcgt tatttttatc
     5881 gcacaagggt gggcatccct gcccgtaatg acgtccctga acctgggtag gttattgata
     5941 ctgaaccttg aaggtcgcat ccgcattagc tgcgccaggg gttgcgacac cggttgcata
     6001 ataacgcgcc tggaacggaa tggtgttagt accgttattc agggttgttt gctcactgaa
     6061 tgtcgcacca tccagcgtca gcgcagcacc cgttctgtcc aggatctgta caccaacgtt
     6121 tgttgcgcta cccgcagctg aactctgcag agccagaacg ttggtatgac ccgcatcaat
     6181 cgccgtacct aaaaaggcaa ctgcggcttt agatgcaaca ttggtatcgc aatcattcag
     6241 ctgaatgtta aaaccgacag cagaactggt tgctccttcc tgtgccagcg atgcggtacg
     6301 aacctgtcct aactgaacga tttgatcaac agagcctgca tcaactgcgc aagcggcgtt
     6361 aacaacttcc cctttaaagt gaacggtccc accattaacc gtcgtggcag cggccagagc
     6421 cgctgtagaa ctgagggaca gagccgacag aacaacgatt gccagagttt taattttcat
     6481 gctgctttcc tttaaaaaaa ctatttctaa atcgacatgg gcagtcgttc tgtacacttt
     6541 gttttgtcaa cgagtttgac tgccaacact gcacagtttt cccccaaaag atgaaacatt
     6601 tggggccatt ttgactcata gaggaaagca tcgcgcacaa actttttcag tttatttgtt
     6661 ggcttaatat tctattgtta tctttattta tagattttta tattgcatga ggtggttttt
     6721 ggagagaaga atgaggaaga tgcgtcgagc cacagaaacg ttagctttac atatagcgga
     6781 ggtgatgtga aattaattta caagagaaat aatttacata tcaaacagtt agatgatttt
     6841 tgtcgttttt taaaattttt atgcttgaga aaaaatacgt aacttattta tgatatggac
     6901 aatttggccc caattgtctt gtatttattt tttaacttta ttatcaataa gttaattcaa
     6961 acctcttctc tttttaattt ttcgtttatg agattatttc tttcccataa tccggcaaaa
     7021 cgagcagcat tactggcggt ataacgtaca gtatggcgaa tatttcgatg cccgagataa
     7081 tcctgaatta aacgagtatc tgcaccacgc tccgccagtt cataaccgca agcatgcctt
     7141 aacatatgag gatgagtctg cgttacggtt ccagcttcaa taccggcatc acgaataatg
     7201 cgataggcct gctggcgaga aagccgactc ccgcggcgag aaataaatat ggcgtcggtc
     7261 cggtcagcgc ctttccagtt agcacgttcc agggtccagc gttccacagc ttcacgctca
     7321 tcaaaacgta acgggtgaac ggtagaaaat ccgttcttca gtcggcgaat atttattcga
     7381 ccttcattaa ggtcaagatc ctgataatgc agatcaagca gttcactaat acgcatccca
     7441 tgccgatatg ccaacagaat aagacaataa tctctggctc ccgttgcccc gtaacaaacc
     7501 gcctgcatca tggcctgaac ttctttaccg gtaagataac gacgtttact cacaatagta
     7561 gtactcctaa ctgagatata tttgaatgaa tacctatagg aaacctcaat cggtcaaaat
     7621 tagcccaata gataaaaaga taaccacaca cgaagtgatg tggctatcag tcaattacat
     7681 taaatatcat acaaaaataa aa
//
LOCUS       DAZPEB010000178         7597 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZPEB010000178 DAZPEB010000000
VERSION     DAZPEB010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7597
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.182"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(1..307)
                     /locus_tag="ACHURJ_003436"
                     /pseudo
     CDS             complement(1..307)
                     /locus_tag="ACHURJ_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842854.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(451..3759)
                     /gene="icsA"
                     /locus_tag="ACHURJ_003437"
                     /gene_synonym="virG"
     CDS             complement(451..3759)
                     /gene="icsA"
                     /locus_tag="ACHURJ_003437"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HFR1282248.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFAIPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSIINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEITGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYGWFNNTVKGDGFTGEKYSSKGITGALEAGYIYPTIRWTTHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGVNNIQAKLGLRTSLISHSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4288..5490
                     /gene="virA"
                     /locus_tag="ACHURJ_003438"
     CDS             4288..5490
                     /gene="virA"
                     /locus_tag="ACHURJ_003438"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HFR1282249.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDVLLKIITFG
                     IYSPHETLAEKYSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFPVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQLNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
     gene            5669..6356
                     /locus_tag="ACHURJ_003439"
                     /pseudo
     CDS             5669..6356
                     /locus_tag="ACHURJ_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    5949..6065
                     /locus_tag="ACHURJ_003439"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
     gene            6358..6724
                     /locus_tag="ACHURJ_003440"
                     /pseudo
     CDS             6358..6724
                     /locus_tag="ACHURJ_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            6727..7125
                     /locus_tag="ACHURJ_003441"
                     /pseudo
     CDS             6727..7125
                     /locus_tag="ACHURJ_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006278 - RNA-templated DNA biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase/maturase family protein"
BASE COUNT         2243 a         1751 c         1515 g         2088 t
ORIGIN      
        1 tttataccta ctctgaccaa gtctggataa gaaaggcaca cctgcacatt tctacttgga
       61 aatgttttct tttaccattt tgctcaaaaa tttcaacatc caatattcct cgtacagaac
      121 tactcaagtc ctgaatagaa aaagttccag ctggtacagt agagtcatat attgctcgac
      181 cttgttgaga tacaacaaca cgtgcattag tttctgcaat accagtaatc tgaggcgcat
      241 aaccacgaat atttggcggt agcattcgtt catcgctttc taatgcaatt cctgtgtaac
      301 gccaagcaac agcgcattct ggcgcatgat ctggtaaaca cgtttggcat tgatcgcagg
      361 cataccatca tgtgcacaaa aacccaccga gcttttacac ggtgggtccc agagaaatgc
      421 aggacatcaa cacgccctgc atttttatta tcagaaggta tatttcacac ccaaaatacc
      481 ttgggtgtct ctgtaactgt tattacccaa ttgctgagat acgtttcccc agatgcttag
      541 gttatctgca agacgccccc ccacaccagt tttgagttca atcacattac ggttgcctat
      601 ctggtgattt gacatgccat tcataattac accatattgc tttgagctcc atttccaatt
      661 cacttccaaa aatggttcga acttacgaag agtctcctta tcgatacaac tatgactaat
      721 taaggatgta cgcaatccca attttgcttg aatattattg accccagagg atgtgaccat
      781 agtgccattg tgttcaatat agtcgtttgc ttttacccca tgcctagtta tctggacttg
      841 tggattgaga tacaatgcgt tgtcaatatt attatgagta gtccagcgta tggttgggta
      901 gatatagcca gcttccaaag ctcctgttat tcctttgctg gaatatttct caccagtaaa
      961 cccatctcct ttgactgtat tattaaacca accgtactgc aaccaagcat ccatatatag
     1021 acctgttcgt tctttttcat cctgaaacca agaactgtac aaaccggcag tataaccgct
     1081 cacagttcct cgtgaactat aactagtata cttattatga gttttgctat cctgatatcc
     1141 ataagctccc ataataccca catgcaagcc atcatgatca gtgaagtttg tcttcaaaat
     1201 atctccacca atctgataaa ccatcctgtt agctgtggtt tttaattgtc tatcagctaa
     1261 attatgctca ttatgtccac caacagtacg aatccacatg gtggtttcgc tgagctgacc
     1321 tgtagtagga tcaacaatag tgctaccgtg tcggtcatag agagaaggac taaacatcgt
     1381 gttcattgct atcaggttag caatataact gccagcttca gggcgccatg tgtgaatact
     1441 accttcaccc aaattaagtg aagatacaag ctgtgaacta gctcggcgac tactcatttg
     1501 agtagactct tgattgctca tctgttttga ttcttgatta tccatctgac tagttagata
     1561 ccacttattg gtattcagtc cagatacagt gccctgtttc aggcgatagt cataactacc
     1621 agcaacaata cggcctttct gaataaaagc attcttatca gaagaatctg tcgaaataat
     1681 ttgaacacct tcaagagttt tatctccaga gcctccctca tttgtcactg caacataggt
     1741 aataccagac gcatggcctg taatttccag ccggtcagtt ttactgttgt cattgccgag
     1801 aaccgcgccc aaagataata ttccgccctt tccatgccaa ttacctttct gaacataggt
     1861 ctgaccgaca tttgacgaac tattattgag aataacatga ccgtttttct ccagagtcat
     1921 gttacctgtg acaatgacgg ggtcaggcaa aaaaggggca ttaatattat tgattagcac
     1981 agtcccacta tttaataaag tgttaacagt ttggttcatg cctgaaaaat tcaaggtagc
     2041 acctttatta acaataacac cagcggtacg tgtcatagct tcaactgtcc ccatttttag
     2101 aatgcctgca ttgatgttgg tgaaaccaga gtaggtattt ttttccgcca aaatgagagt
     2161 tccctcccct gattttgtta gggattttcc gtcccagcta tttatattat gatggctgag
     2221 attatctgcc agaatagtac taacagtaag ttcagcgttt tctttcagat tgaactctcc
     2281 atgactgtcg ccatctgtat cattccagct caaaccgtag atcaatttat tctggtcact
     2341 gatatagctt tttgtatagt caatagcacc agtagtgagt aacggattga tgatagatat
     2401 attagaaaca ctgaaacctg ttatcccttt atctgcactt agcagtttat agtccttctg
     2461 cgcccagtta tttataatag cttccacttt tacattgttg ccaaatgaca tatttccaga
     2521 agcttccaga ctactgattc cagctaaatt taaagaactg ttctcaccaa aagaaacgtc
     2581 acctgaaaca gtgaatttgt tacctgatag tgaaacagtt gtacaatcac cagcacataa
     2641 atcaccttca atagttggtg tggtcccagt gtaatcattg ttgataattt tcagaatgga
     2701 tgaattgtta acctgtacat caccagtaat gacagaacct tcatggagta ttaatgaatt
     2761 gtttccacca gtgatgtgga tagcattacc tgctacattt tgtgttccat catcttcttt
     2821 acctgaaatt atatatccgc tattagcaat ggtcatattg ctaccattta tagcatcccc
     2881 ccaatgtcca ccattaccgc ctaaaatagc tccattgttg attatagaca ggttatctcc
     2941 tgtgatagca ttaccaccgt agccatcata accataaccg taagcagcac ctccgttgcc
     3001 tccagcgaac gtgcccttat tgattacaga gaggctgctt cctgtgatgg catcacctcc
     3061 attaccgcca tcaccctcac cataattgtt acctccatta ccaccggaaa tagtcccttt
     3121 gttgattatg ggtagattga ctcctgtgac ggcatcacct ccattccccc catcactatc
     3181 actataacta tcgccaccat gtccacctga aataatttct ccatttatta taaacagatt
     3241 gtctcctgta acagcctcac caccatcacc gttatggtca gcaccgctac cgccgctacc
     3301 accaagaatc atgccttgat tgattataga caagtcagat ccggtaatag agtcaccacc
     3361 attaccgcca caactattac catcattatt atcaccagca ccaccatgac ctgcgctaat
     3421 cataatatca gagttaatcc gtagcttagt tctagatgca tgagaggggt tggaagaagt
     3481 tatataccac gcatccattc catcttcacc agctcccaag ggagaaagtc catcaacagg
     3541 tgttaataat ttttcataat tgtcctctga aaaatgaagt tcttgagtac ccgaaagagg
     3601 aatagcaaaa gctattggcc ccccgagcaa cagggatgca ccaacaacaa aaggagaaaa
     3661 agacaatttg catgtttttt cctttaccgt aggtaattct ccggcccccc cctgtgaaca
     3721 ttgggtcata ttacaaaaaa atttgtgaat ttgattcatg cactatatta tcagtaagtg
     3781 gttgataaac ccctaaaaag attccgtttt atccggaata aagggacgat atatgcaaaa
     3841 acatattaaa caaagcctca accaaaacat gagagctgtg ttatatatca atgcaacggg
     3901 agaaatttac ctgtgtcttg ctttgaaaaa aagaggggga aagctatatg ttcgataata
     3961 aattaataat tttactatga tagtctgctt cagaccgcaa cttttcagtt ctttctgtac
     4021 gcttgcccaa agtttaacca ctttgttttt ctatacaccc aatggttcta tctgcccctt
     4081 gatgttaatc cccaaagcca ataacatgaa tgtattcata tcacaacttt cctaccagac
     4141 tctccaatgc aataactgca taattgtttt tagtggaaat gtatcgtttt cttaaagaga
     4201 agaataacat tccattttat tatgttccca atttaattat attaatatat attggcatca
     4261 ataggaaaat acatcaggag aaatcaaatg cagacatcaa acataactaa ccatgaaaga
     4321 aatgactcat catggatgtc aactgtcaaa tcaactacag aagtaagttg gaataaattg
     4381 agtttttgtg acgttttact gaaaataata actttcggaa tatattctcc acacgaaact
     4441 ctagccgaaa aatatagcga aaaaaaacta atggatagtt tctcaccatc actatcacaa
     4501 gataaaatgg acggagagtt tgcccacgca aacatagatg gcatttcaat acggctatgc
     4561 cttaacaaag gcatatgttc ggttttttat cttgatggag ataaaataca atccactcaa
     4621 ctttcatcaa aagaatataa taacttgtta agttcactcc cccccaaaca attcaactta
     4681 ggaaaagtac atactattac agctccggta tcagggaatt ttaaaactca caagcctgca
     4741 ccagaagtta ttgaaacagc cattaattgc tgtacatcga taataccaaa cgatgattac
     4801 ttccccgtta aagacactga ttttaatagt gtttggcatg atatttacag agatattcga
     4861 gcatctgaca gcaatagtac aaaaatatat ttcaacaata ttgaaatacc actaaaatta
     4921 attgcagatc taattaatga gttaggaatt aatgaattca ttgatagcaa aaaagagctt
     4981 caaatgctaa gttacaacca attaaataaa ataatcaatt caaacttccc ccaacaagac
     5041 ctatgttttc aaacagaaaa acttttgttt acttcactct ttcaagaccc tgcatttata
     5101 tccgcactaa catctgcatt ctggcaatct cttcacatca cgtcttcctc tgtggaacac
     5161 atatatgccc aaattatgtc cgaaaacata gaaaacaggc ttaatttcat gcctgaacaa
     5221 cgagttatta acaattgtgg acatattata aaaatcaacg cagttgtccc taaaaacgac
     5281 actgcaatct ctgcctctgg ggggagggct tacgaagtta gctcatcaat attaccatcg
     5341 cacataacat gtaatggtgt tggtattaat aaaatagaaa caagctatct cgttcatgct
     5401 ggaacacttc caagcagtga agggcttcgc aatgctatcc ctccagaaag tcgtcaagta
     5461 tcatttgcca tcatatctcc tgatgtttaa gccaatatta tattggctgc cagactgtaa
     5521 aaaaacgttc ttcagaacag ctctataaac tattaaacca aagctaaaag gatggggacc
     5581 aatagaatct cagcccatcc tttttatatc attatgaacc gccccggaaa tcctggagac
     5641 taaactccct gagaaagagg taaacaggat gactaaaaat acacgttttt cccctgaagt
     5701 ccgtcaacgg gcagttcgta tggttctgga aagtcagggc gaatatgact cacaatgggc
     5761 agcgatttgt tccattgctc caaagattgg ctgtatactg gagactctgc gtgtctgggt
     5821 acgccagcat gagcgggata ccgggggcgg tgaggtgggc tcaccaccgc tgaacgtcag
     5881 cgtctgaaag agccggaacg tgaaaatcgt gaactgcgcc gcagtaacaa tatccttcgc
     5941 ctggcttccg cttattttgc aaaggcggag ttcgaccgcc tctggaaaaa ataatgccac
     6001 tgctggataa gctgcgtgag cagtacgggg tcggaccggt atgcagtgaa ctgcatattg
     6061 ccccgtcaac gtattaccac tgtcagcaac agcgacatca tcatgataaa cgcagtgccc
     6121 gtgcgcagcg cgatgactgg ctgaagaaag agatactgcg cgtatacgat gagaatcatc
     6181 aggtatacgc tgtgcgtaaa gtcaggcatc agttgttacg ggaaggtatc agagtggcca
     6241 gatgcactgt ggcacgtctc atggcggtta tggggcttgc cggtgttctc cggggtaaaa
     6301 aggtccacac taccgtcagc cggaaagccg ttgccgcagg cgaccgcgta aaccgtcatc
     6361 aaggcaatgt gccgcgtact ccgggtggcc cgcagcggct ggtatacgtg gtgtcagcgg
     6421 cggacaagga taaacacacg tcagcagttc catcagcact gcgacagcgt tgtccgcaag
     6481 gcttttaccc agtccagcgt tacggtgccc cacgcctgac ggatgaactg tgtgctctgg
     6541 ttaccacttt aacgtaaaaa ccgtggcagc cagtctgcgc cgtcaggaac tgagcgcaaa
     6601 agcctcccag aagttcagcc cgatcagtta ccgtgcacac ggcctgcctg tatcagaaaa
     6661 tctgctgacg caggattttt acgccagtgg cccgaaccag aagtgggcgg gagacatcac
     6721 gtactattac agctcaccga ctgcggggaa acacggggcg ccgggttatt gaaggtgacc
     6781 tgtccagcta cttcgacaca gtacatcacc ggctgctgat gaaagccgtc tgccgcagga
     6841 tcagtgatgc acgttttatg agactgctgt ggaaaacgcc gtgttgatgt tggtctcttc
     6901 cgggcggtca gtgaaagtat gccacagggc ggtgtgatct cgccgttgct gtcgaacatc
     6961 atactgaatg agttcgacca gtatctgaat aaacgctacc tgagtggaaa agccagaaag
     7021 gatcgctggt actggaatca cagtatccag cgaggccgaa gtactgctgt caaagaaaac
     7081 tggcaatgga aacctgccgt tgcttactgt tgctatgccg atgactgtgt gccacgaagg
     7141 cgcgtcctgg ggacgtgagt ccctgtgcag ccatgcggca caagagatga gggaaggccc
     7201 ctcggtgtcg gcgtgcaggc gcaggcacca aaccatccac tgctgctgca tttaagagat
     7261 gtggtggtga gcgagatggg aaaaggtcgg acgtgagccg atcaggtatg gttcgaagag
     7321 acgaacgaaa gtgaaccgct gatgatgtgt cgaaagcata tagatgatgt caaaacaatg
     7381 gtggagtcgt tcctgtggga tatgtttact gcctgcgcgg catccggcat aaaagcggcg
     7441 tgaagtcgat tcaggctctt gtggggaacg tggacctgtc accccgatgt gaagggagaa
     7501 gcgcaagtgg aagccccatg agcgccagag taccaatgcg gggtacaggg acggagcacc
     7561 aagcgattgc gcagtaaaca tgcttgacaa cttaatt
//
LOCUS       DAZPEB010000179         7549 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZPEB010000179 DAZPEB010000000
VERSION     DAZPEB010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7549
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.183"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            85..717
                     /gene="comR"
                     /locus_tag="ACHURJ_003442"
     CDS             85..717
                     /gene="comR"
                     /locus_tag="ACHURJ_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFR1282250.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(779..1318)
                     /locus_tag="ACHURJ_003443"
     CDS             complement(779..1318)
                     /locus_tag="ACHURJ_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFR1282251.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1545..2849)
                     /gene="ndh"
                     /locus_tag="ACHURJ_003444"
     CDS             complement(1545..2849)
                     /gene="ndh"
                     /locus_tag="ACHURJ_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFR1282252.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHSELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTHDPDIYAIGDCASCTRPEGGFVPPRAQAA
                     HQMATCVMNNILAQINGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3249..3791)
                     /gene="ycfP"
                     /locus_tag="ACHURJ_003445"
     CDS             complement(3249..3791)
                     /gene="ycfP"
                     /locus_tag="ACHURJ_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFR1282253.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3814..4839)
                     /gene="nagZ"
                     /locus_tag="ACHURJ_003446"
     CDS             complement(3814..4839)
                     /gene="nagZ"
                     /locus_tag="ACHURJ_003446"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFR1282254.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPEKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSTLIRENKLDAIMPAHVIYSD
                     VDPRPASGSSYWLKTVLRQELCFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKASSTRLNQLHERW
                     QEEKAGH"
     gene            complement(4850..5674)
                     /gene="thiK"
                     /locus_tag="ACHURJ_003447"
     CDS             complement(4850..5674)
                     /gene="thiK"
                     /locus_tag="ACHURJ_003447"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFR1282255.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDTALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(5658..6296)
                     /gene="lpoB"
                     /locus_tag="ACHURJ_003448"
     CDS             complement(5658..6296)
                     /gene="lpoB"
                     /locus_tag="ACHURJ_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFR1282256.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPIVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQ"
     gene            complement(6310..6687)
                     /locus_tag="ACHURJ_003449"
     CDS             complement(6310..6687)
                     /locus_tag="ACHURJ_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFR1282257.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQKPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6690..7049)
                     /gene="hinT"
                     /locus_tag="ACHURJ_003450"
     CDS             complement(6690..7049)
                     /gene="hinT"
                     /locus_tag="ACHURJ_003450"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFR1282258.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVVAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            7383..>7549
                     /locus_tag="ACHURJ_003451"
     CDS             7383..>7549
                     /locus_tag="ACHURJ_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282259.1"
                     /translation="MLSTQFNRDNQHQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDG"
BASE COUNT         1846 a         2030 c         1799 g         1874 t
ORIGIN      
        1 atttttatgg gatctggact ggtgaacgga ggtgattatt tttataataa tctttcattc
       61 acagtcacca ggtacaacgg catcatggca actgactcaa cacaatgtgt aaaaaaaagc
      121 cgtggccgcc caaaagtgtt cgatagggat gccgcgcttg ataaggccat gaaattgttc
      181 tggcaacacg gttatgaagc gacttctctt gcggacctcg tcgaagcgac cggagccaaa
      241 gcgcccacgc tatacgcgga atttaccaac aaagaggggt tatttcgtgc cgttctcgac
      301 cgctatatcg atcgttttgc cgctaagcat gaagcacagc tgttttgtga agagaaaagc
      361 gtggagtctg cgctggctga ctattttgct gccatcgcca actgctttac cagcaaagac
      421 accccggcag gctgcttcat gatcaacaac tgcaccaccc tctccccaga ttcaggagat
      481 atcgccaata cgttgaaatc acgccatgcg atgcaagagc gcactttgca gcagttttta
      541 tgtcaacgac aagcgcgcgg ggaaatcccg acccactgtg acgtgacaca tctggcagaa
      601 ttccttaatt gtattattca ggggatgtcg atcagcgcac gcgaaggtgc atcgctggaa
      661 aaactaatgc agattgccgg aacgactttg cgtttatggc ccgaactggt gaaataagtc
      721 gcaaaaaaag cccctcagcc tgagcgaatg aggggccaaa ttgcagagta cagccttgtt
      781 atactttgtt attcaaaatc agttgcccat tactatctag cgggatctgc gtacccggat
      841 cgcggtccat gcggattttg ccctgctgat cgccaatctt ataggtcaca tcataaccga
      901 gcattttttc tgacttgtca tacaccgttt tacaacgctg ttgcgtagtc gtgtaagtat
      961 cgctttcctg gagagagccc tggatctggt taccggcata tccaccaccc agcgccccca
     1021 caacagtggc gacatcttta ccgcgaccac cgccaaactg atgccctatc acgccgccag
     1081 caacagcgcc gagcaccgac ccggtaatgc gattttcatc ctgcaccggt cgacgatggg
     1141 tcactgtgac gttgcgacac tcctgacgcg gtgttttaac cgtttccttg attggggttg
     1201 cagaaacaac ctgagcgtat tgcgggccac gttcaaacac gttcagactg gccactgccg
     1261 ctacgcccag cgcagctgcg acaccaatcc cgatacccgc caacattgat ttattcacgg
     1321 gaacacctcc ttctttgcag ttatgccaat catgcaagcg aaggagaaaa gcagctatca
     1381 gacagaagat gaaaagcgcg ggaatggctg gaaatactta atgattcgga ggagtctgaa
     1441 ggaatgcctg atgcgacgct taacgcgtct tatcaggcct accgctcgta ccatttgtcc
     1501 agggcggatg aggcgtttat gccacatccg ccagtgtacg tcgattaatg caacttcaaa
     1561 cgcggacgga taacgcggtt aatactcccc accagcatca ttaatccggt tttaaagtaa
     1621 ccatgcagcg caatctgatg cattcggtat agcgagatat atacaaagcg cgcaattcgt
     1681 ccttcaatca tcattgagcc gcgcgtcaag ttacccatca ggctaccaac ggtggagaag
     1741 ttcgacagcg ataccagtga accatgatct ttatactgat aatttttcag cggcttaccg
     1801 tttatctgcg ccagaatatt gttcattacg caagtcgcca tctggtgtgc agcctgagca
     1861 cgcggcggaa caaagccccc ttccggacgc gtgcatgacg cgcagtcgcc aatagcgtaa
     1921 atgtctggat cgtgggtggt ttgcagcgtc ggttccacca ccagctggtt gatacggtta
     1981 gtttcaagac caccgatatc tttcaggaag tctggcgctt tgatcccggc tgcccacacc
     2041 atcagatcag cctcaatata ttcaccatct ttagtgtgca ggccgccttc atcagcactg
     2101 gtgaccatgg tttgcgtcag cacgcgaacg ccaagtttcg ttaactcact atgggccgca
     2161 gcagagatgc gtggcggtaa tgcaggcaaa atacgttctc ccgcttctac tagcgttacg
     2221 ttcagggctt cgttggtcag acctttataa ccgtagctgt gcagttgctt gaccgcgtta
     2281 tgcaactcag cggagagttc tacacccgtc gcgccgccac cgacaatcgc aatgttcact
     2341 ttgccattcg cgcccaggtt ggcggagtat ttcaggaaca gattcagcat ctcctggtgg
     2401 aagcgacgcg cctggtgcgg gttatcgagg aaaatgcagt tctctttgac gcctggcgta
     2461 ttgaaatcgt tagaggtgct acccagcgcc atgaccaggg tatcataggc gattttacgt
     2521 tccggaacca gcagttcacc tttctcgtcg cgtagttctg caatagtgat tgttttcgct
     2581 tcacgatcaa tatcaatgac ggaacccaac tggaactgga aaccatgatt gcgcgcatgg
     2641 gccagatagc tcaacgcatc gacgccttca tcaagcgagc cagtcgccac ttcgtgcagc
     2701 agcggtttcc acaggtggct gtggttacga tcgaccagcg taattttggc ttttttctta
     2761 cgtcccagct tatgccccag ctgtgttgcc atttccagcc caccagcacc gccgccgaca
     2821 atcacaatct ttttcaatgg cgtagtcaac gtgaccccct taaatttatt aaccaattgt
     2881 taattaatgg ttattaacat agctcttaat taacaacagg ttacagcaat gaaaatgttc
     2941 ttcgctattg agaataacat gaatggtgca ttggtcatac caaaattgat gttaatcaag
     3001 ttttgttgct gaaaagatag gcgactttac cgggtgagat caaaaaatag agtttattgt
     3061 ttattatttg agcgaatttg tgagagtgat atagagtgag gggtgtatgc agggcgttcg
     3121 gtaggcctga taagaggcgc atcaggcatt gatgctcgac tgtcggatgc ggcgtaaacg
     3181 ccttatccga cttacagaat tcggagcgca tcaggtaccg gcaaggttac ctgatgcgac
     3241 gagggcattt acccgagggt tttgaacgct ttaatgcgct gcaaatgcgg ggagatattc
     3301 ttgaatttgt gcgtctgctc ttcgtcccag acaatctcgt aataatgatg caactcttca
     3361 gatgtccgct ggctgttaag cgcttcatca ttacgcgaca aaatcaccag gcaacgatcg
     3421 cgattcttct cgcggaagtt ggtcacacac ttagtggcaa tatcggcata ctcttccggg
     3481 cgatcaatct tgccttccat gttctcataa gggaacaaat tagggttgaa gatcacctgg
     3541 cggatatcgc aaagaaaacc aatccgttcc gcccagtatc cgcccagacc aacgccgcaa
     3601 atcagcggac gctcgtcgac gttcagttgc aacattttgt ccacttcttt aagcagatgc
     3661 tgcatatcat gtttcggatg ccgcgtactg tagcttatca agcgtacatc cgggtcaata
     3721 aactgcaatt gtaagacttt ctcgtggtta cccggactgt tagagtcaaa accgtgtaaa
     3781 tagataatca tcgcttcctc acataagcca gggttagtga cctgctttct cttcctgcca
     3841 gcgttcatgt aactgattca gacgggtgct gctcgccttc cagcgagccg agtccatcag
     3901 ttcctgtcgc gaaaatgaac ctttatgata caaacgtgta acacgttctg ccttgatcgg
     3961 tgacagatta tctaacacgc tgacggcccc tttacgatta ttgcagacca ggatcatatc
     4021 gcaacctgcg tccagtgatg cctgcccgcg ttcggcataa ctgcccataa tcgcggcacc
     4081 ttccatcgat aaatcgtcag agaaaattac accgtcaaaa cacagttcct gacgcaaaac
     4141 ggttttcagc cagtaagaag aaccgctcgc cggacgcgga tcaacatcac tgtagatcac
     4201 atgcgcaggc ataatggcgt cgagtttatt ttcgcggatt aacgtgctga aaaccgacat
     4261 atctttggca cgaatttccg cttgcgggcg tgggtcgcac ggcgtctctt tgtgtgaatc
     4321 tgcagttact gcaccgtgtc ctgggaagtg tttcccggtc gttttcattc cggcttcatg
     4381 catcccatca ataaaccgac tagcgattgc cagggctttt tctgggtcgg catgataaga
     4441 acgctcgcca atcgccgcgc tgatatgtcc tacatccagc actggcgcaa agctgatatc
     4501 aatatccata gcgatcattt cgctggccat cagccaaccc gcctcttgcg ccagtttgcc
     4561 gccctcttcc attcccaaca gcgcagcaaa ggattgtgct gccggtaagc gggtaaaacc
     4621 ttcgcgaaaa cgctgcacgc gtccaccttc ctgatctacc gccaccacca gatgattgcg
     4681 cgatgctgcg cggatctggc gcaccagttc acgtaactgg gcaggatcat gatagttacg
     4741 cgtaaagaga atcagccctc ccaccagcgg atgcgccagt atttcacgct cttccgcgtc
     4801 cagttcataa ccttcgacat ccaacattac tggacccaca ctgctctcct tattgtttta
     4861 ttaatagctg ccgccaggtg tcatcggcca gcctgataaa ttgttgatcg ccggtttgtc
     4921 gccagcggta ctcaaaccac cctgctttga gcatcagcag ccagggaaac catcgcctga
     4981 cctgacgcca taattgcgcc ggataaatct tcgcgcgagt ggcatagtca ttgaccaatt
     5041 gccggtgctg ttcagtattt tccacccaca ccgccgccag ttccagcgcg gtatcaccat
     5101 ctccggcata ctcccagtcg atgagtttta accctgatgc gctatgcact aaatttccgg
     5161 cgtggacatc catatgcaat ggacttaagc gtaaaggtcg tggttcccgc gctttgcgca
     5221 gacgttttaa cattcgcagc caacccacag tccgccgcgc cggatcgctt tgctgccagt
     5281 acagttccag taacggcaac agcgttattc gccagccaaa acgtggttgt tgatgtagat
     5341 aatacagcaa gcctgccagt tcgttggtat ccggcaaata cgtttttacc gcgccgggca
     5401 gatagtcgac taccatccag tcacggagat ataaatgcgg cttcggtgca atgcttgcgg
     5461 gtagttgtga taaagcccga tactggcgca agaacgcgga ctgcggcgca tcaggatcgt
     5521 gcggctgacg cacaacaaaa cgctggccct gatgttcaat gagaaaacta ccgccactaa
     5581 gcccactatt aaacgtcgtg acgggatgaa actgcgggaa aaagcgcgac agcaattcgt
     5641 cgcgcgtgat gggattatta ctgcgaaacg gcacctttac ctgaccagat aatttcgccc
     5701 gtctgcacca gcatcagctg catttgtagg gtcggagcgt taacgttgcc agaggcgctg
     5761 gagtacagca cgtaatgagc gccgacattg cgggcaatgc ctatggcttt actacgggtg
     5821 cctaaactgt cctgcggcga caaacctaac tgttgcttag ccatcgacag ttgctgggcg
     5881 gaaaccaggg taaatttccc gttattagcc agcgcatttc gcagcgtttc ggtcgcttct
     5941 gcggcattca gcgaaccgtt agtacggttg ttaacgctat caaccagcag gacgctaccc
     6001 gcagtcaccc cgtcagcccc aagcatctta ctgactatcg gctgcattgc gccattccag
     6061 tcataatggc gaatatgcgg cgcaggcggt gccgttcgat cttcgtgctc aattgggcct
     6121 ggctgctgcg ggatcgtcgg caccgagggc actgtgggga caggctgttg tggctcggct
     6181 ggttgttccg gcgctggttt cacttcttct accggtgcag gttcacgttg ccccacacac
     6241 ccggcgagaa acatcgccag cgcggtaatc aaggcgtagc gactcatttt tgtcatcaag
     6301 attcacccct tacaaatata gataaagtct gactttgtgc gcccccagaa aattggcgct
     6361 gccgtacagc gttaccgccg aatgtgcggg aatggtaacg ctgcgtggcc tttccagagg
     6421 atgcatctcc agccctctgg cgtcatacca gtaaaaacga taatgaacgg taacgggttt
     6481 ttgcctttcg ttataaagcg ttgaggatgc tgaaggttga atatcagacg tcgaaaggac
     6541 gggcttttct gcactgatgc ccgcagccaa taaagatgac tccatcacca gcgattgctc
     6601 atcattcacc ggaatttccg gatgtgaacg acagcccacc agcagtaaca acaccagaga
     6661 caccagccca aagcatcctt ttctcatcgt tacagacctt tatgcgccag cattggtccc
     6721 agcggacggc cacccaacaa gtgcatatgg atgtggtaaa cctcttgtcc gccatggcgg
     6781 ttggtattca tgatcagacg atagccatct tcggcaatac cttcttgctc agcaattttt
     6841 gccactacgg tgatcatgcg tcccagcgcc tgctcatgct cagctgagac gtcgttcaca
     6901 gtcggaatga ggatattcgg aatgatcaga atatgcgttg gcgcttgcgg cgaaatatcg
     6961 cgaaacgccg ttaccagatc atcctggtag acgatatcgg aggggatctc acgacgaata
     7021 attttgctga atatagtttc ttctgccacg acattttcct ttttcaaata gccatgcgtt
     7081 gcgccatgct acgccgcgcc acactgcgag tatagagtat ggtcgagtta aacctgctct
     7141 ttcaacctta actcacgatt tcttaagcaa aaaatcgttt tgctgccgta tatatcgcca
     7201 ttattcccat ttctgcctgc gaatgcgctg aaaagacatt ctctgtttgg tttacacgac
     7261 aaatatgaat gcgtatattt ctcatttgca tttacaaaca aaattattcg cacactaaat
     7321 ataatcgctc atcaccctcc tccggcatga gcctgacgac aacataaacc atgagatttc
     7381 aaatgctttc aacacaattt aacagggata atcaacatca agccatcacc aaaccgtcac
     7441 tacttgccgg ttgcatagca ctggcactat taccttctgc cgcttttgct gcaccagcca
     7501 ctgaagaaac ggtgattgtt gagggttcag ccacagctcc agatgatgg
//
LOCUS       DAZPEB010000180         7527 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZPEB010000180 DAZPEB010000000
VERSION     DAZPEB010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7527
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.184"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            3..590
                     /locus_tag="ACHURJ_003452"
     CDS             3..590
                     /locus_tag="ACHURJ_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR1282260.1"
                     /translation="MNQLADKQTIKQTLFARQPAKIHKPYPTNSKIIMGDVLNHAALK
                     QAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVLSLGIYDEVPGKFGEWNN
                     AVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYELTSRNEPFKGTIVSRKS
                     VAALITDIIDKPEKHIGENIGINQPVTDGDKPLFM"
     gene            900..1628
                     /gene="mpaA"
                     /locus_tag="ACHURJ_003453"
     CDS             900..1628
                     /gene="mpaA"
                     /locus_tag="ACHURJ_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFR1282261.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(1603..2568)
                     /gene="ycjG"
                     /locus_tag="ACHURJ_003454"
                     /pseudo
     CDS             complement(1603..2568)
                     /gene="ycjG"
                     /locus_tag="ACHURJ_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
     gene            2687..3193
                     /gene="tpx"
                     /locus_tag="ACHURJ_003455"
     CDS             2687..3193
                     /gene="tpx"
                     /locus_tag="ACHURJ_003455"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFR1282262.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAFGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3237..4778)
                     /gene="tyrR"
                     /locus_tag="ACHURJ_003456"
     CDS             complement(3237..4778)
                     /gene="tyrR"
                     /locus_tag="ACHURJ_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFR1282263.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(4926..5987)
                     /locus_tag="ACHURJ_003457"
     CDS             complement(4926..5987)
                     /locus_tag="ACHURJ_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFR1282264.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREICRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(5984..7381)
                     /locus_tag="ACHURJ_003458"
     CDS             complement(5984..7381)
                     /locus_tag="ACHURJ_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFR1282265.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDTWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
BASE COUNT         1860 a         2078 c         1812 g         1777 t
ORIGIN      
        1 tgattaatca actggcagat aaacaaacca ttaaacaaac tttatttgcc cgtcagccag
       61 caaaaatcca caaaccctat ccaactaata gcaagattat tatgggagat gtactgaatc
      121 atgcagcact gaagcaggcc atgcagggac aggacgttgt gtatgccaac cttacggggg
      181 aagatttaga tattcaggca aattcagtga tcgctgcgat gaaagcctgt gatgttaaac
      241 gtctgatctt tgttctttcc ctcggtattt atgatgaagt tccaggaaaa tttggcgaat
      301 ggaataatgc agttattggt gagcccctaa aaccattccg cagggctgcg gatgcaattg
      361 aggcttctgg actggaatat acaattcttc gccctgcctg gctgactgat gaagatatta
      421 ttgattatga gcttacctca cgcaatgaac cattcaaagg tacgatcgta tcacgtaaga
      481 gtgtcgcagc attaatcaca gatatcatcg ataaacctga gaaacacatt ggtgagaata
      541 taggtatcaa tcaacccgtg actgatggtg acaagccatt attcatgtaa atcaattaca
      601 tagttaccat cgtaatgtac tatgtgcagt acgatttatt aaacttatta aggtgaactg
      661 atcaatccga tgcgattaac atgtgtagaa ccagtaagaa taaaatacgc catgtatata
      721 acattacgac ttacatctaa gtcagcataa ttctaatatc tgcacatcaa taaaaatgca
      781 aacaacttcc ggcaaaaaaa gtgatacaag atcgatgaca tagcgttgaa gaaaaggatt
      841 tgataatccg caaaatttgc acaacactgc ctgtaatact tcctgaacca gagacgatca
      901 tgaccgtaac ccgcccacgc gccgaacgcg gcgcatttcc gcccggaaca gaacattacg
      961 gacgttcatt attgggcgcg ccgttaatct ggtttcctgc ccctgcagcc agccgcgaaa
     1021 gtggtttgat tctggctggc actcacggtg atgaaaactc ttcggtcgtc acgctctcct
     1081 gtgctctgcg gacgttgaca ccttctttgc gccgccatca tgtggtgctg tgtgtgaatc
     1141 ctgacggctg ccagctgggg ttacgggcca atgctaatgg tgtggattta aacagaaact
     1201 ttccggcggc gaactggaag gaaggtgaaa cggtttatcg ctggaacagc gccgctgaag
     1261 aacgtgatgt cgtattactg acaggtgaca aacctggctc agaacctgaa acccaggcgc
     1321 tgtgccagct tatacaccgc attcaacctg cctgggtggt ctcattccac gatccactgg
     1381 cctgtattga agatcccaga cacagcgaat taggcgaatg gctggcccag gcgtttgaat
     1441 tgcctctggt aaccagcgtc ggttatgaaa cgcctggctc tttcggcagt tggtgtgcag
     1501 atctcaactt gcattgcatc accgcagagt ttccgcccat ctcctccgac gaagccagcg
     1561 aaaaatatct ctttgcgatg gcaaacttgc tgcgctggca tcctaaagat gcaattcgcc
     1621 cgtcgtgaac tgaagcgccg gttccacatc taccgccagc caggtcggtc cgtcaagatc
     1681 ggcgaaactg acctgcggca ccagcggtaa agcggcgcta atggcacgag aggtacacaa
     1741 catgcagccc agcatcagac tgaaaccttg tgcacgcgct tcagtcgcca gcgccagcgc
     1801 ttccgtcaga ccaccggttt tatcgagctt aatgttaacc atctcatagc gccctttcag
     1861 cgccttcaga ttgctacgtg tatgacaact ctcatcagca caaatcggca acggatgaat
     1921 aaaattctcc agcgccgcat cgtcctgcgc aggaaggggt tgttcaagca tcgcaacgcc
     1981 taaatccgcc aatagctggc aacgcgccgc caacccttct gcacgccagg attcatttgc
     2041 atcaacgatc agcgtcgcat cgggcacagc tgtgcgaatt gccaccatcc gctcactaat
     2101 aagatggtta tccagcttca ctttcagtaa tttcgcgcct gcctgccaga gtgttgatgc
     2161 actattggcc atctgatcag gcgtaccgat gactaccgtc tgtgcagtga taactgtctc
     2221 gggaagcgtt atgccgatca aatcagccag cgattgctgc tgttttcgcg cggccagatc
     2281 ccacaatgca caatccagcg cattacgtgc tgcgccggca gggagaattt tttgcaactc
     2341 ctcccgtgtc agccctttct ctaattgcga cacgacgctc ataatttgcg ccattaccga
     2401 ggcatcactt tctccataac gcggatacgg cgtgcattcg ccggtgcctt taataccctc
     2461 ttcttccagt tcaaccacca ccacgcgcgc ttcactgcga cttccccggg caatcacaaa
     2521 cggggtatgt aagggctagg cttcctcaaa taccttaacg gttctcatct ttactccttg
     2581 ttgtcgcggt ggtctgcgga ttaagcgcaa atagcgtttg ctgtgttatt gacagttagc
     2641 ataaactagg tgtgacgtta actatatgta aacaggaaag ataatcatgt cacaaaccgt
     2701 tcatttccag ggcaacccgg ttacagtcgc caattccatc ccgcaggcgg gtagcaaagc
     2761 gcagactttt actctcgtgg caaaagatct gtctgacgtc accctcggtc agtttgcggg
     2821 taaacgcaaa gtgctgaaca ttttcccgag tattgatacc ggtgtttgcg ccgcatcagt
     2881 acgtaagttt aaccaactgg caaccgagat cgacaacacc gttgtgctgt gtatttctgc
     2941 cgatctgccg ttcgcccagt ctcgtttctg cggcgcagaa ggtctgaaca acgttatcac
     3001 cctctccact ttccgtaacg ctgaatttct gcaagccttc ggtgtggcaa ttgctgatgg
     3061 cccactgaaa ggtctggcag cgcgtgccgt tgtggttatt gacgaaaatg acaatgtgat
     3121 tttcagccag ctggtggatg aaatcaccac cgagccggat tacgaagcag ctctggctgt
     3181 actgaaagca taagttaata tgcctgatgg tgttgcacca tcaggcatat tcgcgcttac
     3241 tcttcgttct tcttctggct taagccatat tcccgcaact tattggcaat cgcggtatgt
     3301 gaaacgccga gacgttttgc cagtttgcgc gtgctgggat aattgcgata aagctgggtt
     3361 aataccgagc gttcaaaacg gctggtgatt tcgtccagcg aaccttccat cgcatcttcg
     3421 cccacggcta ccgttgcggc gtcataatcc ggcaacaaaa tatcctgtgg acgcagctca
     3481 taaccgtcca gttgtgtcag tgcgcgatat atagcgttct ttaactgccg cacatttccc
     3541 ggccacgcat aacgcgtaag tacagtattc aggtcagcgg ccagtttcgg acgcggcacg
     3601 ccctgctcgt cggcaaagcg ggcgacgaac agctcagtta acggcatgat gtcctgcgga
     3661 cagtcacgta gcggcggcag attgagcgtc aacacgttca gacgataata gagatcttca
     3721 cggaacatgc ctttttgcac cagttcgacc agattcttct gcgtagcgca aatcacccgc
     3781 acatcgacat gcacctcatg gtcttcgcca acccgacgga aagtgccatc attaagaaaa
     3841 cgcagtaatt tcgcctgcat ccgtggtgac atttccccta tttcatccaa cagcaccgaa
     3901 ccaccgttcg cctgctcaaa gaatcctttc ttcccttccg gagcatgacc aaacagttca
     3961 ctctcgaccg catcttccgg tatagacgca cagttcagcg ccaggtaagg tttgcccgct
     4021 ctggggcttg cctgatggca ggcgtaggca aagagatctt tacctgtacc tgtgtcaccc
     4081 gtaatcagca gcggcgcgct tagcatcgcc agtttctgcg cctgttcgac aacatgcttc
     4141 attttcgggc tgacggcgac aatttgactg aaggcgctga cgtcctgggc ggcgacattt
     4201 tgcaactggc ggcccatacg aatcgttgat cgcaacatca ccaccgcacc ggtcaggacg
     4261 tgttgatcat tttcatcctg aagataaaca ggcgtaatct ccatcaggaa attctgccca
     4321 ttaataacga catgctcgtt atgcgaatct tgcggttcgc tttccagcca acgtaaaaaa
     4381 ttaaagccgt taatcaattg tgcggcggta tggttgcgca ggcgatccaa tttttgccca
     4441 aaaagctgac agctcgccgg gttcgccata tccactttgc ttttcatatc gacagagagc
     4501 acaggttcgg gcaacgcctc cagtaacgcg ctcaacgcca gatgctcacg ttcggaaggc
     4561 atccacggga cagtacgcac atcggtaaca cctgcaatac ggcgtatttc ggccatcaga
     4621 ctgctgaaac tctcaaactc cagttcagca aaattgaggt agattcgccc aatgggatca
     4681 atctcaatac cgcgtaaatc aatgcctctt agcacgagta gatcgagtaa ttcgcgggtc
     4741 agaccgagtc ggtcttcaca aaagacttcc agacgcatgg gaaccttcac ctgaaaaaag
     4801 aacaattaat atgatgatat gacactattt ggatagcagg aaggtttctg tcaacaatca
     4861 ttgacacaaa gacggaaaaa cgggcacttc ggcgtaaaga tatccggtca gcacccgata
     4921 ttgcattatt tttcgctggg cgttttgcct ttttgcagcg tttctttcac ctgaccgata
     4981 agctgacgac ggaaatcccc gaggcgaggt ttgtcatcgt caatccacgg cagcgggcgg
     5041 caaagctcca tagctttaat cccgagtcgt gccgtcagaa gtccagcacc aattccctga
     5101 gctgcgcggg tagacaaacg agcagcgaga tcttgcgaca tccagtccat ccccacttcg
     5161 cgcaccagtt cgctggctcc ggcaaaagcg atattcagca ataccagctt aaacagacgc
     5221 aaacggctgt aataccccag ttcaatgcca tacagcgtgg cgatgcgatt aattaaacgc
     5281 agattgcgcc aggcgataaa cgccatatcg accaacgcca gcgggctgac cgcaatcatc
     5341 aacgttgatt ccgccgccga acggcagatt tcgcgccgcg cctgggcatc taaaactggc
     5401 tggaccaaat gcgcatacaa actgaccact tcacggtcgt tttgcgtttc atggattgag
     5461 gcataccagc gttgcagcgc tggatgagac tgatcaatac ccgcctgctg cgccagtttt
     5521 tcgcaaaatg cgcggccttt gcccgtgccg tggctgtgca acaaatcgcg cgcttcgtcg
     5581 cgttcatggg cgcgctgtcg caagcgccat aagcgccgcc actctgttac cacagaacct
     5641 acgccagcgc cgataatcaa tgccccagcg gcacatccac ccagcgccac ccagtcctga
     5701 gtttgccagg cattcattgt ccactgtaca ccctgcccga caacgcttgc gccaaacaga
     5761 gccagcccgc ccatcaccat tttgcgccac aggctgcgtt tcggacgtaa cgctgcatcc
     5821 attaccgctt caacttgccc ctcttcttca ggcgcttcat cgagcgtggc cggggcaaaa
     5881 ttttgcgcct gattttcgtc aaaggtctgc tgcgccctga attttggatt ctgatcgacc
     5941 tccagcggac cgtcgaaatc aatacgtggt tttaacggtt cggtcatcgc aatttatctc
     6001 ctattaaaaa ttccagcgcg gcatcaagac gaatatgcgg cagcggtttg tcgacatcca
     6061 tcacctgcgg gcgaaacgct tcaaactgga acccttgttt gtcccagaac gcctgaccgg
     6121 gcaaacgcgc aggaacttca ccgggataaa cggtgagtgg cgcaccatcg ctgagccggt
     6181 taccgcgcaa tgccggaatt ttctcgccgt tgacgtcaat aatgccgctg gtggtcgcct
     6241 gcaccgaggc cagccccagg caatccatac taatcccttc aaacgccgca ttttgccagg
     6301 catcctgaat cagttgttgc agtaatgaaa ccatattggc gtgctgatcg atggtcacat
     6361 ggtccgcttt agtggcagca aacaatagct tatcgataac cggcgaaaac aaacgccgga
     6421 acaaggtacg ctgcccgtag tggaaacttt gcatcagctg cgtcagtgcc agacgcatat
     6481 cattaaatgc ctgtggccca ctgttgagag gttgcaggca atcaaccagc acaatctggc
     6541 ggtcaaagcg cagaaaatga ttcttataga accccttcac caccttctcg cagtaataat
     6601 taaaccgctc gcgcagcatt ccggcattgg tgtgcttatc ggcctgcgcc agtttggact
     6661 cgccccaggt atcgacatcc ggccacggga agaattgcag cgcgggcgca cctgccatat
     6721 ctcctggcaa gacaaagcgc cctggctgaa taaagtgcag cccctgctgt ttacagtggt
     6781 ggagataatc ggtccacgcg gcggcaatgt ctgccagccg gttttcgtcg gcaggtgcta
     6841 gcgggtccag cccttcgcac atcattcgcc atttgaccga ccattcgccg cgctgaccat
     6901 tgagtaagcc cgtcatctgg cgtgaccagc ttaaatagtc ctgcgccagc atcggcaggt
     6961 cgagcaacca ttcgccggga taatccacaa tctccagata cagcgtggag gtgtccttga
     7021 agtggcgtag cagcgaatcg ttcgatttaa aacgtagcgc caggcggatt tcactgacgc
     7081 cgcgcgttgg cgtcggccag gcgggaggat cgccatataa ctgcgccagt ccttcatcat
     7141 atgtgaagcg cggaatgcca aagtcacgct gaggaatgcg ttttacgccc agcaggcgct
     7201 cttcacgcac cgcgcttaac agcggcaagc gtgctccggc gtgaatattg agcaactgat
     7261 tgaccatagc agtgataaac gctgttttgc cgctgcggct tagtccggtt acggcgaggc
     7321 gcagatgtct gtcgacaccc cgattcacca gcgcattaag ttcattttta agtcgcttca
     7381 ttgctgtcct gtgctgctct gaagaccaga aaaaaatcat tcgtaaggat atgaatgtgg
     7441 gggccaggcc attgattatc aactgcccgt gttgtcatcg acgggcgaac tccataagat
     7501 gtgcagcatt cacgttcgga cggagtt
//
LOCUS       DAZPEB010000181         7473 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZPEB010000181 DAZPEB010000000
VERSION     DAZPEB010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7473
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.185"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..339
                     /locus_tag="ACHURJ_003459"
     CDS             <1..339
                     /locus_tag="ACHURJ_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFR1282266.1"
                     /translation="QTEAFLNAYGLSRFAPLGYDPRDLPIRDLAGYRKKGNHDGDPII
                     FYTFPAAFEQEIAKGFNTKQFAEVLKNAGMLTPPTSGRGYQGRVREDGRQIRVYVLNF
                     MAEESSQPEE"
     gene            714..1148
                     /locus_tag="ACHURJ_003460"
     CDS             714..1148
                     /locus_tag="ACHURJ_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFR1282267.1"
                     /translation="MTAQISAYGRLVADPQTRTTTNGNNMAMARLAVSLPCNAAEAGE
                     STFWLGVIAFGKQADALAKHQKGDLVSVAGNMQLNQWTGQDGGTQQGYQVVADSVISA
                     RTVRPGGKAGQRGQATDALRRAQQPSRDEYDQRPPFDDETPF"
     gene            1159..1800
                     /locus_tag="ACHURJ_003461"
     CDS             1159..1800
                     /locus_tag="ACHURJ_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002436679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282268.1"
                     /translation="MSTQPIIIEFGSYRWPRHLAIHEADHAIAAWFFGIQEVEIALSG
                     NNRVAKTYLSGDVDECGAVAVWHANYPRNVAEVKSYTLSNDDTRLRLIDQVCRAMVIS
                     LAGIVAQSRYTGDDINALMETSGKADMKNVRDLSEVYRVAGGEDADIQRISLSRAEAL
                     IALKWWDVIALAKILENRNHMPASNFQRIMGDIDKPVPKPMTLAELDELVGEE"
     gene            1797..2147
                     /locus_tag="ACHURJ_003462"
     CDS             1797..2147
                     /locus_tag="ACHURJ_003462"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282269.1"
                     /translation="MKPYILAAGLISSAPALSAIHCGGFTIQPDRYRTTINGDVVHIV
                     DAQFYGAPRDFSQAAIKLLPDAITTTDRQYRLETQGGNALLELMTRENPPRVLNRERC
                     DAGLTSFGWYTSAP"
     gene            2841..3452
                     /locus_tag="ACHURJ_003463"
     CDS             2841..3452
                     /locus_tag="ACHURJ_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282270.1"
                     /translation="MRDRTRSYTTDLPRIGLPFLANMRSRLADAEPGTQLYTQTESGT
                     LYAFRQADSYAMTINGVTRAIRTTTTKAGYGVREWYACPHCMKRAAKLYIGKKDIGCR
                     ACWKLHYKSQSADILDRMRMKIRQQRCAIWGSNDLANNLFNDIRMFPKPKGMRWATFD
                     RKRAELPVMEMAYWQAFSPVVDRITGVIERKAGKAARGIGLTL"
     gene            3684..3869
                     /locus_tag="ACHURJ_003464"
     CDS             3684..3869
                     /locus_tag="ACHURJ_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282271.1"
                     /translation="MTPREIALLTIAKLEHGGHQLTQADQREIERSVNADIARRDRFR
                     EMMRAPAYQWKKPAPRR"
     gene            3990..4532
                     /locus_tag="ACHURJ_003465"
     CDS             3990..4532
                     /locus_tag="ACHURJ_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HFR1282272.1"
                     /translation="MYQLKITIRDSKPPIWRRVLVPEQIPFSELHAVIQLAFGWNDEH
                     LYMFEKGRKGDPGSEYRVWGEDESMGNAAITPLWAALQNDGDKLVYTYDFGDWWDCVI
                     VLEKQTHDTSNQPISCLRGKGTTPVENSGGLHGYNELLLQARESDNPEQAEIHNFLML
                     DIERRVYDLSSINDRLQTLN"
     gene            complement(4525..4884)
                     /locus_tag="ACHURJ_003466"
     CDS             complement(4525..4884)
                     /locus_tag="ACHURJ_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282273.1"
                     /translation="MRMTSRKKEILSYFEPDNLEWVTGEIGAPPFDVSGVAYLLHGMV
                     SFDKRHQLESTRRTLESMVAGGLLERVTVYESRQIRRGGETNATVVRYGLPGQCAVMR
                     DTGGADNAISGEYMRVS"
     gene            5318..6142
                     /gene="dinD"
                     /locus_tag="ACHURJ_003467"
     CDS             5318..6142
                     /gene="dinD"
                     /locus_tag="ACHURJ_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFR1282274.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKNQNILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGRKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            6434..7051
                     /locus_tag="ACHURJ_003468"
     CDS             6434..7051
                     /locus_tag="ACHURJ_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFR1282275.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(7048..7473)
                     /gene="ligB"
                     /locus_tag="ACHURJ_003469"
                     /gene_synonym="yicF"
                     /pseudo
     CDS             complement(7048..7473)
                     /gene="ligB"
                     /locus_tag="ACHURJ_003469"
                     /gene_synonym="yicF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312549.2"
                     /note="this ligase is similar to LigA but it lacks the
                     C-terminal BRCT domain; catalyzes strand joining of nicked
                     DNA in the presence of a divalent cation and NAD+;
                     internal stop; incomplete; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
BASE COUNT         1998 a         1836 c         1909 g         1730 t
ORIGIN      
        1 cagacggagg cgtttttgaa tgcctatggg ctgagtcgat tcgcgccgct gggttatgac
       61 ccgcgcgatt tacccatccg tgatttagcc gggtacagga agaaggggaa ccacgacggc
      121 gacccgataa ttttctacac cttcccggca gcgtttgagc aggagattgc aaagggcttc
      181 aataccaaac agtttgccga agttctaaag aatgcaggaa tgcttacacc accaacgagc
      241 gggcgaggct atcaggggcg agtaagggag gatgggcgac agattcgcgt ctatgtactc
      301 aatttcatgg cagaggaaag cagccagcca gaagaataat tatttcttca catgcgtagt
      361 tttagtgttg tctcggttgt ctttgttgtc tcaatttgta attgagattg atttataagg
      421 attttatttt aatttctgag acaacaccga gacaacattt agccattttg agacaacagg
      481 gcgaaagtgt gtttttagtc tggcaggcag tgagacaaca tttatgccgg gtgagacaac
      541 acgaaattgc aaatgttgtc tcagcaaaac ccagacgtgg cgcggcctgt agagccgtga
      601 gacaacaaag acaacagaga caacactatt tttgcttact atgaaaaaaa atcggcaggt
      661 gtcccacatt aagaaatcgt cattagcaaa actaataatc cagtgagggg aaaatgacag
      721 cacaaatttc agcatacggg cggctggtgg cagatcccca gacccgcacc acaacgaacg
      781 gtaacaacat ggctatggcg cggctggcgg tgtctctccc ctgtaatgcg gcagaggcgg
      841 gagagtcaac cttctggctg ggcgttattg cttttgggaa gcaggccgat gcgctggcaa
      901 agcaccagaa aggcgacctt gtgagcgtgg caggcaatat gcaactcaac cagtggacag
      961 ggcaggacgg cggtacacag caggggtatc aggtagtagc cgacagtgtg atcagcgcca
     1021 gaacggtacg ccccggcggg aaggcaggac agcggggaca ggcaacggac gcgctgagac
     1081 gtgcgcagca gccatcccgt gatgaatacg accaacgccc accattcgac gatgaaacgc
     1141 cattctgaga gggaagatat gagtacgcaa cccatcatta ttgagttcgg cagctatcgc
     1201 tggcccagac atcttgcaat acatgaagca gatcatgcga tagccgcatg gtttttcggc
     1261 atacaggagg tagaaattgc gttatcgggt aataaccgag tggcaaaaac ctaccttagc
     1321 ggcgatgttg atgaatgtgg agctgtcgcg gtatggcacg ccaactatcc acggaatgtg
     1381 gctgaggtta agtcttacac cctctcgaat gatgacacca ggttacgtct gatagaccag
     1441 gtatgccggg ctatggttat atctctggct ggtatcgtcg cgcaatcacg ctatacgggg
     1501 gatgatatta acgcactgat ggaaacgtcc gggaaagcag acatgaaaaa tgttcgtgat
     1561 ttatcagagg tataccgggt ggctggtggg gaggatgctg atatacagag aatatccctt
     1621 tcccgtgctg aggcgctgat tgccctgaaa tggtgggatg ttatcgcgct ggcgaaaata
     1681 ctggagaacc gtaaccatat gcctgccagc aacttccagc gcattatggg tgatattgac
     1741 aagcctgtac caaagcctat gacgctggct gaactggatg aattggtggg tgaagaatga
     1801 agccatacat tctggcagcc ggcctgatct cctcggcacc ggcactatct gctatccatt
     1861 gtgggggctt taccatacag ccagaccgtt atcggacaac gattaacggc gatgtggttc
     1921 atattgttga tgcgcaattt tacggcgcac cgcgagactt tagccaggcc gccataaagt
     1981 tattgccgga tgccattacc actaccgata ggcaataccg cctagagacg cagggaggca
     2041 atgccttgct tgagttgatg accagagaaa acccgccacg tgtgcttaat cgtgagcgct
     2101 gcgatgccgg gttaacaagt ttcggttggt acacttctgc gccgtgatgt tcgttccgag
     2161 cgcagcctaa aatattggac tgttcttgac ggatttaaaa ctctaatgga gccaagtcct
     2221 tgccagtagc gggctaggag cctaattgtg attcaggtcg tcaaaaatat tcaggtttgc
     2281 ggcattggtt cgcagtacgc agtccggtac gcggcaaaac tgcgcattta acgtgcgtac
     2341 cacctgacga attctaacgc cggcattgca aacagatgcg tgcgcaaaat ggctaagacg
     2401 tctgatattg ctggctttgg ctcaatcagt ttggaggtgg cggtatcagt acgatgctaa
     2461 tttttgctaa ggttttagta cgcaactcag gtacgcactc tcaccagata cgaaaccgat
     2521 tcgcagttgc gtaccagcct gtaaggcgca ttatcgctgg gtttctacag tcgaatgcag
     2581 tacgcaaagt ggaaaatcct ccgaatacga aacccgagca ttgcgcagta aattgcgaat
     2641 tgcgcagtta acaaaatatc tccgcaaaac gaaacggata tcaatgcgca aaccatcctc
     2701 aacccccgcg cggttactgg ctttattgat gtgaaattgc aaaattggtg cgcaatttac
     2761 agtaaaatac gcatattatt tcgtattcaa tttgcgcacc gttcgcagtt aatgcgcagt
     2821 aattcgcagg aggccagcca gtgagagaca gaacgcgcag ctataccacc gacctgcccc
     2881 gtatagggtt gccattcctt gcgaacatgc gtagcaggct ggcggatgct gaaccgggta
     2941 cgcagcttta tacccagacc gaaagcggaa cgttgtatgc cttccggcaa gccgacagct
     3001 acgccatgac aatcaacggc gtgacgcggg ctattcgcac cacaaccacg aaggccgggt
     3061 atggcgtccg ggaatggtac gcttgccccc actgtatgaa gcgggctgca aagctgtata
     3121 tcggcaagaa agacattggc tgccgggcgt gctggaaact tcactataaa agccagagtg
     3181 ccgacatact ggacaggatg cgcatgaaga tacgccagca gcgatgcgcg atatggggca
     3241 gcaatgacct tgccaataat ctgtttaatg acattcgcat gttcccaaaa ccaaagggga
     3301 tgcgctgggc tacgtttgac aggaagcggg cagagttacc agttatggag atggcttact
     3361 ggcaggcatt cagcccggta gtggacagaa tcacgggcgt tattgagcgt aaagcaggaa
     3421 aagcagccag aggcattggc ctgactctat aaaagcaaaa cgcccgcacg gggcgtcaga
     3481 gaacgggata gacttctgcg acaatttgca ttcgccgcca atatagcaag ccgtccacat
     3541 caagcaataa actatcaccg ggagggggag gccaaatctc tgcaatcctg agccttaagg
     3601 actgcccgcc ccatcgtttt ttcatacccg cgaaaaatga aatttaatcc gggcgtgttt
     3661 catccttcca gagggtatga cgtatgacac cacgcgaaat agccttattg accatcgcca
     3721 aacttgagca tggaggccac cagcttacac aggcagatca acgggagata gaacgctcag
     3781 ttaatgccga tatagcccgg cgcgacaggt tccgcgaaat gatgcgagca cctgcctacc
     3841 agtggaagaa gccagcgccg cgcaggtaga tacgggatta ttccgcatgt aacttatgcg
     3901 tattaagcaa atttccttta ttcaccttta cagaattgtg agtacgtcag tatcgttatc
     3961 cttccgattc gcatttattc aggatttgca tgtaccagct aaagataacc atccgagaca
     4021 gcaaaccacc tatatggcgg cgtgtgctgg ttccagagca aatccccttt agtgaacttc
     4081 acgcggtgat ccagttggct tttggctgga atgacgaaca cctgtatatg ttcgagaaag
     4141 ggcgtaaagg tgatcccggt agcgagtatc gcgtatgggg tgaggatgaa agcatgggta
     4201 atgcggcaat cacaccacta tgggcagcgc tccagaatga tggtgacaag ctggtttaca
     4261 cgtatgactt tggcgactgg tgggattgcg tcattgtgct ggagaagcaa acacacgata
     4321 cgagcaatca gcccattagc tgcctgcgtg ggaaaggcac caccccggtg gaaaactcag
     4381 gcggcttgca tggttacaat gaattgctat tacaggccag agagtctgat aatcctgagc
     4441 aggccgagat ccacaatttc ctgatgctgg atattgagcg ccgggtttac gacctgagca
     4501 gcatcaacga cagattgcag actcttaact gacccgcata tattcgcctg atattgcgtt
     4561 atcagcgccg ccagtatcgc gcataacagc gcactgaccg ggcaaaccgt agcgcacgac
     4621 cgttgcgtta gtctcaccgc cgcgccgtat ctggcgcgat tcatacactg tcacccgctc
     4681 aagcagtcca ccagcaacca tgctttccag tgtgcgccgg gttgattcga gctggtgacg
     4741 cttatcgaac gacaccatgc catgaagcag gtaggccaca cccgacacat cgaacggcgg
     4801 cgcaccaatc tcaccagtca cccattcgag gttatccggt tcaaagtagc taagtatctc
     4861 ttttttgcgg ctggtcattc tcatagctgg caggttcctt attgtgggat agcactatca
     4921 tacaataagg cgcaaaagat aagaggccag aacagcaagc atgagagcat aaaacgagca
     4981 gccaaagaca gcgcctcaaa ataccgtgta cacaagggtg tacctactga gaaaacgcat
     5041 tacaagaaac acagcaacca cgggcgttta aagccactac tcaataaaca gattcaactc
     5101 gtaacgccaa ttcttacttt tcagccttca caaatgccgc ccactcaaac agagcggcat
     5161 ttttcttccc cgcaacttca attctgtttt gcgtgcctgc tccagatttt gcgatgtttt
     5221 tttgcccagc acactgagaa cgtgagatac tcacaactgt atataaatac agttacagat
     5281 ttactttctt tgcaatcgat atcacatgga gtgggcaatg aacgaacatc atcaaccttt
     5341 tgaagaaata cggcattatg gcactgaagg gcaagaattt tggtcagcta gagaattggc
     5401 accgttactg gattatcggg actggcgcaa ttttcaaaag gtactggccc gcgcgactca
     5461 agcgtgcgaa gccagcaatc aagctgcatc tgaccatttc gttgaaacca ccaaaatggt
     5521 tgtattaggt tcaggggctc aacgagaact tgaagatgtt catctctccc gctatgcctg
     5581 ttacctggta gtacaaaacg gcgaccctgc gaaaccggtt attgcggcag ggcaaactta
     5641 ttttgctatc cagacccgac ggcaggagct ggctgacgat gaagctttca ggcaacttcg
     5701 tgaagacgaa aagcgtcttt ttctgagaaa tgaattgaaa gaacataaca aacaattggt
     5761 tgaggcagcg cagcaagctg gggtagcgac agctatcgat ttcgccatat ttcagaatca
     5821 tggttaccgg gggctgtatg gtggattaga tcagaaagct attcatcagc ggaaggggct
     5881 gaaaaagaat cagaatatcc tggatcatat gggttcaaca gaactggcgg ctaatctctt
     5941 tcgagctacc caaacagaag aaaaactcaa gcgggatggc gtgaattcaa aacagcaagc
     6001 aaacaccaca cactttgacg tgggtcgcaa ggtgaggcaa accattcagg aacttggcgg
     6061 aaccatgcct gaggagttgc cgaccccgca ggtcagcatc aagcagttgg aaaacagtgt
     6121 aaaaattaca gagaagaaat agcagaaact aaaaactctt tttcttaatt gagatacccg
     6181 atgcgtaagc caaagtccag gtgcagtgaa cacaatggct aaacattgca cctttctttt
     6241 ccacccctca attttaacct attttttctt atgcattttc tcagacaaga actcagaata
     6301 atgcatctac gctacagaaa atagcgattt cacataacta caagttatca atttcctctt
     6361 accttaaaaa atctcaatcg tgacaatgcg cacaaatcgc taccctgcca gacagatttt
     6421 tagggagaga accatgctgt tacacatttt gtatttggtt ggcatcactg ccgaagccat
     6481 gacgggggcg ctggcggccg gacgacggcg catggataca tttggcgtaa ttattattgc
     6541 gaccgccacc gcaattggcg gagggtcagt gcgcgatatt ctgctgggcc actatccgct
     6601 cggctgggtc aaacacccgg aatatgtgat tatcgtcgct accgccgcag tactcaccac
     6661 tatcgttgct cctgtaatgc cttacctgcg caaagtgttt ctggtgctcg atgcgctcgg
     6721 actggtggtc ttttctatca tcggcgcaca ggttgcactg gatatggggc acggcccaat
     6781 tattgccgtt gtcgcggcgg taaccaccgg cgtgtttggc ggcgttttgc gcgatatgtt
     6841 ctgtaaacgc atcccactgg tattccagaa agagttgtat gctggtgtct catttgcctc
     6901 cgccgtgctg tacatcgcgc tgcaacacta tgtttctaac catgatgtgg tgattatctc
     6961 caccctggta ttcggctttt ttgcccgctt actggcgcta cgtcttaagc tgggattacc
     7021 ggtcttttac tacagccacg aaggtcacta aggttcaaaa cctgtgatct gctgggcagc
     7081 cagccaactg cccagcttct tgatttgcgc attttccttc cattcaataa cctgtctggc
     7141 gcgcccagat ccagtccccg gcagctgctg tcagaactgc tccgtgctaa ataaaagttg
     7201 cgactaggac cgttcatcac tggcattaag cgccgcccgg gttagcggta ttcccattgc
     7261 catcacccag cgagtaaaag gctgcttacg agccagatta aactgatgcc ataactgcgc
     7321 acttttactt ttcgcgatcc ccggcgtgtt ctgtaattgc tctggcgtta ataaaagcca
     7381 agaaaagata tgctcaaagc gatgagtctg atgcagcgcg cgccaaccgg cctcaccaat
     7441 gccatccagc ccaagaacct gttttgaccc cag
//
LOCUS       DAZPEB010000182         7435 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZPEB010000182 DAZPEB010000000
VERSION     DAZPEB010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7435
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.186"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            167..274
                     /gene="yniD"
                     /locus_tag="ACHURJ_003470"
     CDS             167..274
                     /gene="yniD"
                     /locus_tag="ACHURJ_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFR1282276.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(327..1085)
                     /locus_tag="ACHURJ_003471"
     CDS             complement(327..1085)
                     /locus_tag="ACHURJ_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFR1282277.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVTYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            1372..2301
                     /gene="pfkB"
                     /locus_tag="ACHURJ_003472"
     CDS             1372..2301
                     /gene="pfkB"
                     /locus_tag="ACHURJ_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFR1282278.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISASQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            2402..2692
                     /gene="ghoS"
                     /locus_tag="ACHURJ_003473"
     CDS             2402..2692
                     /gene="ghoS"
                     /locus_tag="ACHURJ_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFR1282279.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            2798..3658
                     /locus_tag="ACHURJ_003474"
     CDS             2798..3658
                     /locus_tag="ACHURJ_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFR1282280.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(3699..4235)
                     /locus_tag="ACHURJ_003475"
     CDS             complement(3699..4235)
                     /locus_tag="ACHURJ_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFR1282281.1"
                     /translation="MTYQQAGHIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            4382..5050
                     /gene="hxpB"
                     /locus_tag="ACHURJ_003476"
     CDS             4382..5050
                     /gene="hxpB"
                     /locus_tag="ACHURJ_003476"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFR1282282.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            5213..5803
                     /locus_tag="ACHURJ_003477"
     CDS             5213..5803
                     /locus_tag="ACHURJ_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR1282283.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQIINTLQIQFHRLIKHQVEY"
     gene            5936..7327
                     /gene="tcyP"
                     /locus_tag="ACHURJ_003478"
     CDS             5936..7327
                     /gene="tcyP"
                     /locus_tag="ACHURJ_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFR1282284.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(7331..>7435)
                     /locus_tag="ACHURJ_003479"
     CDS             complement(7331..>7435)
                     /locus_tag="ACHURJ_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282285.1"
                     /translation="FADDLFKYESVCIAAMEHLKSKATAPIQNGPLPA"
BASE COUNT         1866 a         1810 c         1837 g         1922 t
ORIGIN      
        1 attttcgata tatatgttga tgcataatat tattttttca taaaacgcag aaacagtgac
       61 caggtgtaca acgccaccca acggctgtaa taacaaaaaa tcccttgatg cccgtccctt
      121 ttgttacact ccgttatcac gcacaagaga tatgcaggac actggtatgc cgactaaacg
      181 ctttgataaa aaacactgga agatggtggt ggtgctactg gcaatctgtg gcgctatgtt
      241 gttgctacgt tgggcagcaa tgatttgggg ctgagcagtg acaaacgaag acagccagac
      301 cgcataacac ggtccggcag ataattttac atgctgtagc ccagcgataa ggttgtccga
      361 cggtcggtat gttccggtgc agactctgga ggttcagagt tccatgtaac gttgtaagtc
      421 accttcagtc caaaatgttc attaatagca acatttaatg cgctttcgga gttcagcgtt
      481 gtgtcttccg cgccaaagac cgaaacaccc tgcgtaaatt tagcgttgtc agtcaactgc
      541 catgcataag ccccggaggc atagcccagc ggctgagttt cactggcatt gtcggtatat
      601 ttgtcgtaac gcacacctgg accgaattca aagcggaaac tgtgtaccgg gccattcaaa
      661 aactgacgac cataacccgc ggtcaacaca tcgcgctctc gatagccgtt ataacggtct
      721 gtcagccagc ttgcctgacc aaataaatag tcataatcag ttaaattaaa gcggctacgc
      781 ccgcccgccg catatttttc tgaagaacgc tcatcattag aagaagtatt actggcgttc
      841 ccccacagcg accaggcagt ggtttgtcca taccaggtca tggtggtgtc agccgtaagt
      901 gaggagcttt tcgtattgcc tgattgtgca agatatcctg cgttcagatt accttcgaaa
      961 ggttttttag cgctggcagg atcgtccatg acagtaaaaa cggaatcatc ggcagctgca
     1021 ttcagtgacg caaacatgcc ccccgccaac ataacgatgg caggaactgt cttcaaaagc
     1081 ttcatttatc aagagtccgt acaacaaaaa aagagaccat cgcggtcccg gaaactttct
     1141 taaggatcaa agattagcgt ccctggaaag gtaacgaatt ataaaaaggc gcgaataact
     1201 tagcaatgta ttcttatttc attttttgaa taagcatgtg gcgaaaacag atttttattt
     1261 atatatattt atctgcaaaa ttttaaataa agctccaata aatcatattg ttaatttctt
     1321 cactttccgc tgattcggtg ccagactgaa atcagcctat aggaggaaat gatggtacgt
     1381 atctatacgt tgacacttgc gccctctctc gatagcgcaa caattacccc gcaaatttat
     1441 cccgaaggaa aactgcgctg taccgcaccg gtgttcgaac ccgggggcgg cggcatcaac
     1501 gtcgcccgcg ccattgccca tcttggaggc agtgccacag cgatcttccc ggcgggtggc
     1561 gcgaccggcg aacacctggt ttcactgttg gcggatgaaa atgtccccgt cgctactgta
     1621 gaagccaaag actggacccg gcagaattta catgtacatg tggaagcaag cggtgagcag
     1681 tatcgttttg ttatgccagg cgcggcatta aatgaagatg agtttcgcca gcttgaagag
     1741 caagttctgg aaattgaatc cggggccatc ctagtcataa gcggaagcct gccgccaggt
     1801 gtgaagctgg aaaaattaac ccaactgatt tccgcttcgc aaaaacaagg gatccgctgc
     1861 atcgtcgaca gttctggcga agcgttaagt gcagcactgg caattggtaa catcgagttg
     1921 gttaagccta accaaaaaga actcagtgcg ctggtgaatc gcgaactcac ccagccggac
     1981 gatgtccgca aagccgcgca ggaaatcgtt aatagcggca aggccaaacg ggttgtcgtt
     2041 tcccttggtc cacaaggagc gctgggtgtt gatagtgaaa actgcattca ggtggtgcca
     2101 ccaccggtga aaagccagag taccgttggc gctggtgaca gcatggtcgg cgcgatgaca
     2161 ctgaaactgg cagaaaatgc ctctcttgaa gagatggtcc gttttggcgt agctgcgggg
     2221 agtgcagcca cactcaatca gggaacacgt ctgtgctccc atgacgatac gcaaaaaatt
     2281 tacgcttacc tttcccgcta acaaaaacat tcccccagca ttgggggaat caccaccaac
     2341 ctgtcggcaa cgcgtttctc cgactatgct caaaagtcat gtgataacaa aggggtgaac
     2401 tatggccagt ggcgatcttg tccgttatgt cataaccgtc atgttgcatg aggatacatt
     2461 gactgaaatt aacgagttga ataattacct gactcgcgac ggttttttgc tcaccatgac
     2521 ggatgatgag ggaaatatcc atgagctggg gactaacact tttggactta tcagtaccca
     2581 aagtgaagaa gaaattagag aactggtttc ggggcttacc caaagtgcaa ccggcaaaga
     2641 tcctgaaatc accatcacga cctgggagga atggaatagc aacagaaaat aaatggtttt
     2701 tgggcaataa tcagtctgtg gtgtgcgtta gctcgtgttt ttacaccgca ttcttgcgct
     2761 aaccttatga tctggcagac aacatgggag agacatcatg tggcaggcaa tcagtcgtct
     2821 tttgagcgag cagttaggtg aaggcgaaat cgaactgcgt aatgaactgc ctggcggaga
     2881 agtccatgcc gcatggcatt tgcgctatgc aggacatgac tttttcgtca aatgtgatga
     2941 aagggaactg cttcccggtt ttaccgccga agccgaccaa ctggagttac tgtcgcgtag
     3001 taaaaccgtc accgtgccta aggtttgggc agtaggcgct gaccgtgact acagttttct
     3061 ggtgatggat tatctcccac ctcgtccgct ggatgcgcat agcgcattta ttcttggtca
     3121 gcaaattgcg cgtttacatc aatggagtga ccaaccacaa tttggcctcg attttgataa
     3181 ctcgctctcc acaactccac agcccaacac ctggcaacgt cgctggtcaa cgttttttgc
     3241 tgaacaacgg attggctggc agttggaact ggcagcagag aaagggatcg ctttcggcaa
     3301 tatcgacgcc atcgtcgagc atattcagca gcgtctggcc tcgcatcaac cgcagccttc
     3361 tctgttgcac ggcgatttat ggtccggcaa ctgtgcgctg ggtccggatg gcccgtacat
     3421 tttcgaccct gcctgctact ggggtgaccg agagtgcgac ctggcgatgt taccgctgca
     3481 tactgaacag ccgccacaaa tctatgacgg ctatcagtca gtatccccgc tacctgccga
     3541 tttccttgaa cgtcaaccgg tttaccaact ctacacgctg ttaaatcgtg caaggttatt
     3601 tggcggtcag catttggtta ttgctcagca gtcactggat agattattag cagcatgata
     3661 tgggttgaag ataatggccg ctctgtgcgg ccttttgatt aaataaaccc aagcagagaa
     3721 aagaacacat aacctgcagc aatacaaata accggcagga tatacagtga gaaaaactgc
     3781 aggaaaatag tatggtgcgg aacaacaata cgggactcaa tttgttcacg cgttagcccc
     3841 tcttcccctt tggctttttc cagaatgagt tgatcttcaa caccttcccg caagaagcgc
     3901 gcctggcgac tcattcgggc accggaatcc tgcaacgcca gtccgacaaa aatcaggatg
     3961 aaaatcaccc agaacataac gttcacgcca ccattaaaat tgggcgtcgg ggagttatac
     4021 cagaacagat tcaaaaaagg cgtattcgcc tgcatcatat cgatcatgac atgagtaaag
     4081 tcgagcatga ccgcattaat gccttcctgg ttttcctgcc gggtattcat aaatttcagc
     4141 aaagaaatca atgtagagat caatgcaggt ataaaaatca cccaacccaa aatccttttc
     4201 aaaacagcaa tgtgtccagc ttgttgatac gtcatgagtt ctccttgatt aagacgcgtc
     4261 gtttcactta gtttacctgt agatatctgt tttcgcccat tctttaaagg cgatatgata
     4321 ggcgcttaat cataagcacg gcttaatacc ttacacataa tgctctaaag gagaggttgt
     4381 aatgtcaacc ccgcgtcaga ttcttgctgc aatttttgat atggatggat tacttatcga
     4441 ctcagaacct ttatgggatc gagccgaact ggatgtgatg gcaagcctgg gggtggatat
     4501 ctcccgtcgt aacgagctgc cggacacctt aggtttacgc atcgatatgg tggtcgatct
     4561 ttggtacgcc cggcaaccgt ggaatgggcc aagccgtcag gaagtagtag aacgggttat
     4621 tgcccgtgcc atttcactgg ttgaagagac acgtccatta ttaccaggcg tgcgcgaagc
     4681 tgttgcgtta tgcaaagaac aaggtttatt ggtgggactg gcctccgcgt caccactaca
     4741 tatgctggaa aaagtgttga ccatgtttga cttacgcgac agtttcgatg ccctcgcctc
     4801 ggctgaaaaa ctgccttaca gcaagccgca tccgcaagta tatctcgact gcgcagcaaa
     4861 actgggcgtt gacccactga cctgcgtggc gctggaagac tcggtaaatg gcatgatcgc
     4921 ttctaaagca gcccgcatgc gttccatcgt cgttcctgcg ccagaagcgc aaaatgatcc
     4981 acgttttgta ttagcaaacg tcaaactctc atcgctgaca gaactcaccg caaaagacct
     5041 tctcggttaa tgaccagggg cagtgatcac gctgcccctg gttctttatc tgaatttgca
     5101 ttcaactgac ggattaatcg tcaatttaag agaaagagtt acaccgtcac cacttccgtg
     5161 cactgtataa aaatcctata ctgtacgtat cgacagttta gtgagtttta tcatgacggc
     5221 ggaaggtcac cttctctttt ctattgcttg tgcggtattt gccaaaaatg ccgagctgac
     5281 gcccgtgctg gcacagggtg actggtggca tattgtccct tccgcaatcc tgacgtgttt
     5341 gttaccggac atcgatcacc caaagtcgtt tcttgggcag cgattaaaat ggatatcaaa
     5401 accgatcgcc cgcgcttttg ggcatcgtgg ttttacccac agtctgctgg cggtatttgc
     5461 gctgctggca accttttacc ttaaggttcc ggaaggctgg ttcattccgg ctgatgcgct
     5521 acaaggaatg gtgctgggtt atttgagcca catacttgcc gatatgctga cacccgccgg
     5581 tgttcccctg ctctggccat gccgctggcg tttccgcttg cctatcctag ttccccaaaa
     5641 gggcaaccaa ctggaacgtt ttatctgtat ggcattattt gtctggtcgg tatggatgcc
     5701 ccattcatta cccgagaaca gcgctgttcg ttggtcatcg caaataatca ataccttgca
     5761 gatccagttt catcggctta ttaagcatca ggttgaatac taaaaagaca aaaatcacct
     5821 ttctggaata agcaattcca tttgaatata agagccagct cacagttctg ttaatcttgc
     5881 gccaacacta tgactgctac gcagtgatag aaataataag atcaggagaa cggggatgaa
     5941 ctttccatta attgcgaaca tcgtggtgtt cgttgtactg ctgtttgcgc tggctcagac
     6001 ccgccataaa cagtggagtc tggcgaaaaa agtgctggtg ggtctggtga tgggtgtggt
     6061 ttttggcctt gccctgcata ccatttatgg ttctgacagc caggtactta aagattctgt
     6121 acagtggttt aacatcgttg gtaacggcta tgttcaactg ctgcaaatga tcgttatgcc
     6181 gttagtcttc gcctctattc tgagcgcggt tgcccgtctg cataacgcat ctcagttagg
     6241 caaaatcagt tttctgacca tcggtacgct tttgtttacc acgctgattg cggcgctggt
     6301 cggtgtgctg gtcaccaacc tgtttggttt gacggctgaa ggtctggttc agggtggtgc
     6361 agaaactgca cgtctgaacg ccattgaaag taactatgtt ggtaaagtct ctgatctgag
     6421 cgttccgcag ctggtcttgt cctttatccc gaaaaacccg tttgccgatc ttaccggagc
     6481 caatccgacg tcaattatca gcgtggtaat ttttgccgca ttcctcggcg tagctgcatt
     6541 gaaactgctg aaggatgatg cgccgaaagg tgaacgcgtc ttagccgcta tcgataccct
     6601 acaaagctgg gtgatgaaac tggttcgcct tgtcatgcag ttgacccctt acggcgttct
     6661 ggctctaatg accaaagtgg ttgcaggttc taacttgcaa gacatcatca aactgggaag
     6721 tttcgttgtc gcgtcctacc tcggtctgct gattatgttt gcagtgcatg gcattctgct
     6781 gggcattaat ggcgtgagtc cgctgaagta cttccgtaag gtatggcctg tgctgacgtt
     6841 tgcctttacc agccgttcca gtgctgcgtc tatcccactg aatgtggaag cacaaacgcg
     6901 tcgtctgggc gttcctgaat ccatcgccag tttcgccgcc tctttcggtg caaccattgg
     6961 tcagaacggc tgcgccggtt tgtatccggc aatgctggcg gtgatggttg cgcctacggt
     7021 tggcattaac ccgctggacc cgatgtggat tgcgacgctg gtcggtattg ttaccgttag
     7081 ttccgcaggc gttgccggtg tcggtggtgg tgcaactttc gccgcactga ttgtgctgcc
     7141 tgcgatgggc ctgccagtaa ccctggtggc gctgttaatc tccgttgaac cgcttatcga
     7201 catgggccgt acggcgctaa acgttagtgg ctcgatgaca gctggcacgc tgaccagcca
     7261 gtggctgaag caaaccgata aagccattct ggatagcgaa gacgacgccg aactggcaca
     7321 ccgttaattc ttatgctggc aatggtccgt tttgtatagg ggccgttgct ttacttttta
     7381 aatgttccat cgcggcgatg catactgact catatttaaa cagatcgtca gcgaa
//
LOCUS       DAZPEB010000183         7429 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZPEB010000183 DAZPEB010000000
VERSION     DAZPEB010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7429
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.187"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(128..607)
                     /gene="folK"
                     /locus_tag="ACHURJ_003480"
     CDS             complement(128..607)
                     /gene="folK"
                     /locus_tag="ACHURJ_003480"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFR1282286.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQDRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDELSKW"
     gene            complement(604..2001)
                     /gene="pcnB"
                     /locus_tag="ACHURJ_003481"
     CDS             complement(604..2001)
                     /gene="pcnB"
                     /locus_tag="ACHURJ_003481"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFR1282287.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(2061..2957)
                     /gene="gluQRS"
                     /locus_tag="ACHURJ_003482"
     CDS             complement(2061..2957)
                     /gene="gluQRS"
                     /locus_tag="ACHURJ_003482"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFR1282288.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWLHEQGLSYYCT
                     CTRARIQSIVGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQVEAHWQDFSVEQILQSAVK
                     NWTLTAVPESAIVNSTFSNASC"
     gene            complement(3024..3479)
                     /gene="dksA"
                     /locus_tag="ACHURJ_003483"
     CDS             complement(3024..3479)
                     /gene="dksA"
                     /locus_tag="ACHURJ_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFR1282289.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3657..4361)
                     /gene="sfsA"
                     /locus_tag="ACHURJ_003484"
     CDS             complement(3657..4361)
                     /gene="sfsA"
                     /locus_tag="ACHURJ_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFR1282290.1"
                     /translation="MEFSPLLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATRGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMNVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4376..4906)
                     /gene="thpR"
                     /locus_tag="ACHURJ_003485"
     CDS             complement(4376..4906)
                     /gene="thpR"
                     /locus_tag="ACHURJ_003485"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFR1282291.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALMQ"
     gene            4980..7409
                     /gene="hrpB"
                     /locus_tag="ACHURJ_003486"
     CDS             4980..7409
                     /gene="hrpB"
                     /locus_tag="ACHURJ_003486"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFR1282292.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLEELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDDAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGLLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQSPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
BASE COUNT         1703 a         1916 c         2065 g         1745 t
ORIGIN      
        1 tgggattgat atttatctgc gggaatttat attatgtttc tgcaaaaatg tttaaactaa
       61 aatttttatt aaggaaagag aggatgtatg attttcagaa aatatttata ttcgtaatat
      121 aaataaatta ccatttgctt aattcgtcaa atgctcttgt atgtaagact tcacgcaacg
      181 tctccccatc aggaaacgcc agctccggtg caatctcaaa cagcggccac agcataaatc
      241 cacgattctt catatcgtag tgcggaacgg tcaggcgttc ggtatttatc acttcattac
      301 caaacagcat gatatcgaga tccagcgtgc gtggtcccca gcgttcagct ttacgaacac
      361 gatcctgttg cagttctatg cgctgtgtat ggttgagtaa ctcttccggg gcgagcgaag
      421 tttcaagcgc cacggcggcg ttgaggtaat cgggttgatc ttgcggcccc agcggtggag
      481 tgcggtaaaa cgaagaaacg gcaagaatgt ggctttcagg gatatcgcct aatgctttca
      541 gggcagcatt gacctgctcc agcggagagg ccagattgct gcctatggca atatacgcca
      601 ctgtcatgcg gtaccctcac gacgcggtgc gcgtttgcgt ggacgacgag tacggcgacg
      661 cggtgacggc tcttcatcca actcgttaag catccctttt tgatctggtg gcgcggaaac
      721 ctggaactca ccccaccatt tcaccagacg ctgcagttca gcgttacgtt caacttcagc
      781 tcgcaaggcc aacaggtcat aagccgcacg gaacttagga tgctccagca gtttccatgc
      841 gcgtttaccc tgacgacggg acatacgcaa ctgcaactgc cagatatcgc gggttaacgt
      901 cgtcagacgt ttcgggattg ccagtgaacg gcaggcttcg tccagcacgt cgttcatcgc
      961 cagcgcgaaa gcgtcgtgat aggtcaggcc gctttcctgg gcgatcttct gtgccgtctc
     1021 cagcagtggg taccagaaca tggcggcaaa caagaacgcc gggttcacgc gcatatcgtt
     1081 atggatacgc gtatcggtat tcttcagcac ctgttcaatg atccgctcca tcgggctgtc
     1141 gccattttcc gtgaagtagc gggtaatggt cgggaacagc ggctggaaca gatgatattc
     1201 acacaacagc ttataggttt cgtaaccgta gcccgcttgt agcagtttaa gcgattcttc
     1261 aaacaggcgt gccggtggga tatcgttcag cagggtagcg aggcgaggga tcggttctgc
     1321 ggtttccggg ctgatgcgca tacccaattt ggcggcaaaa cgtaccgcgc gcagcatacg
     1381 taccggatct tcacggtagc gcgtttccgg gttaccaatc agacggataa cgccgtcctt
     1441 cagatccttc atgccgccaa cgtaatcacg gacggtaaaa tccgctacgc tgtaatacag
     1501 gctgttgata gtgaaatcgc ggcgctgggc gtcttcttcg atggagccga aaatgttgtc
     1561 gcgcagcaac atgccgtttt gcccgcgttg ggaggtcgtg cggtcgctga cgttaccttc
     1621 gtggtgtcca cggaaggttg caacttcgat aatctccggg ccaaacatca catgagccag
     1681 acggaaacgg cgacccacaa ggcggcagtt acggaacagt ttgcgcacct gctcaggcgt
     1741 ggcgttagtg gttacgtcaa aatctttcgg ctttttgcca agtaacaggt cgcgcacgcc
     1801 gccgccaacc agccaggctt cgtatcccgc tttattgagc ctgtacatta ccttcagggc
     1861 attttcactg atatctttgc gggaaatagc atgctgctca cgcgggatca ccgtcacctg
     1921 tggacgggcg actgcctgtt cagcctcgct ttcctcgcgg cttagcacct tgcggcaaaa
     1981 attagcgact cgggtaaaaa tagtacacct cggtagtgtc aaacatcatt caggacaaaa
     2041 aaatagcggc taatcatagc tcagcatgac gcatttgaga atgttgaatt tacaattgcc
     2101 gactcgggca cggcggtcag cgtccagttt ttgacggctg actgaaggat ttgctcgacg
     2161 ctgaaatcct gccagtgtgc ttctacctgc tgccccagaa attgaagtgc cgcgattagt
     2221 accgggcgtg gatcgccttt cggcaacgca ggcgcatgat tctgcttgga aagtttagcg
     2281 ccttgtggat taagcgccag cggcagatga atgtaatctg gcactttcca gccaaaaagc
     2341 tggtacagcg agatttgcct tactgttggt tcaatcagat cagccccacg cactatttct
     2401 gtaacgccct ggaaatgatc atcaaccaca acagccaggt tgtaggcgaa caacccatca
     2461 cggcgatgaa tgataaaatc ttcccgtgcc agtttttcgt cggcgtgaat aatgccgcgc
     2521 agctggtcag taaattgcgt gaccggatgc tgctggcgga tacgcactgc ggcgttgtct
     2581 ggtccatgat gcaacacccg gcaatgaccg tcgtaaatac cgacaatgct ttgaatacgc
     2641 gcacgcgtac aggtgcagta ataacttagt ccttgttcat gtaaccaggc gagtgcttca
     2701 cgataggcgt cgtgacgttg cgattgccag agaacatcgc cgtcccagtg cagaccgtaa
     2761 tgttccagct ggcgcaggat agtttctgcg gcaccgggaa cttcacgagg cgggtcgata
     2821 tcttctatgc gtaccagcca gcgaccttgc cgggcgcgag cctgcaaata gctgccgagc
     2881 gcggcgatca gagagccaaa atgaagctcg ccggaaggag agggggcgaa gcggccaata
     2941 tactgtgtgt ctgtcatctc tttgaacaaa aaataaggcg ggagcatttc ccgcctgtgg
     3001 taaacgtgat ggaacggctg taattagcca gccatctgtt tttcgcgaat ttcagccagc
     3061 gttttgcagt cgatgcacag atcggctgtc gggcgcgctt ccagacggcg aataccaatt
     3121 tcaacaccgc aggattcgca gtagccgaaa tcttcgtctt ccactttttt cagcgtcttc
     3181 tcgatctttt tgatcagctt acgctcgcga tcgcggttac gcagttcgag gctgaactct
     3241 tcttcctggg ctgcacggtc taccgggtcc gggaagttgg ctgcttcatc ctgcatatgt
     3301 gtaacggtgc gatcgacttc atccctgagt tgattacgcc atgcttccag aatacgacgg
     3361 aagtgcgcca gctgggcttc attcatatac tcttcgcccg gcttctcctg atatggttcc
     3421 accccagcga tggcgagaat actcagggac gatgttttac ggttttgccc ttcttgcatg
     3481 ttgcttctcc ttaacacgca ctatcgatcc ccatgttcgg gggaaaaatg aggccgctat
     3541 aaatagcaga tgcttttccg gatagcaatt atctaaacgt aacacttgac aactgtgtga
     3601 ggaaaagcgt atttgcgcac gcgaccagaa tgtaaattaa ccagttactt actttactac
     3661 aatgtaaccg gcagtgattt tttaagagcc atgccttcag cagaaatttc cgctttgtaa
     3721 gccagaattt ctacccccct ctgttgagct tctgacaata gttgcgcgta tttctcatcg
     3781 atgtggcgcg cgggtgaaaa ccgtgtaatg gctgaatgca gcacggcgaa aaagataacc
     3841 gcacgctggc cttcagccgc tacgttcatc aactcccgaa ggtgtttctg acctcgttca
     3901 gtgaccgcat cgggaaaata tccctgttcg ttctccgcta acgtaaccga tttcacttca
     3961 atatagcagt ctggacgcga atccgcctgc aacataaagt caatacggct gcgttctgcg
     4021 ccatatttta cttcgctttt cagcgagcta tagcctgaca gttctgaaat tgattcatta
     4081 aggatagcct ctttcgtcaa cctgttagcc caaagcgtat tgacgcaaat aaatgcgccg
     4141 ctctggcttt gagttagttc ccaggtgtgt gggtatttcc gtttggtgtt gtctgaagtc
     4201 gaataccaga cggtatcgcc acgcgttgca caaccggtca tcgcacccgt attcgggcag
     4261 tgtagcgtta attcgcgacc atcgggtgtg atcacatcgg ctaaaaaacg tttgtaacgc
     4321 tgaattagcg tcgcgcgctg tagaagggga gagaattcca ttgcgacaat ccttgttatt
     4381 gcattagcgc ccagcgtttt agcggcgtgt agcgtgtgcg tccacgggca aacgaggagg
     4441 cgtaaagggt gaactccgtc accgcataag accagttaaa acctggcggc gggattgtca
     4501 ccgcctcgct ggcgtcgcgc aataaggtaa tatgtggatg aaacggacga ttgctttgaa
     4561 aacaaccgct gcgggcagcc tgtgaacgga gcatattcgc cagctggatt aagccgcgtg
     4621 gcggctgacg catccctaac cacaccacac gcgaacgcag ccattgtccg gcgtcatcaa
     4681 gcgtgagtgt gaaaccaggt tgacgaatcc gtccggctaa aagagaaagc gccttctctt
     4741 tctcggcgct cacttcgcct aaaaatgcca gagtcagatg caaattatcg gcggcgaccg
     4801 gacgtcccgc ctcaggtggg aagtgtgtgg cgcgccagtg gataatctgt tcgcggattt
     4861 ctgcaggtaa gtcgatagca aagaacagac gttgcggttc agacatgtga ggcactcggt
     4921 tatgaattac cgcgatgcta caatgtggcg cgaagaatgt taaccctctg gagcgttttg
     4981 tgtcgtcgtt gcccgttgct gccgttttac ctgaattact taccgctctc gattgtgcgc
     5041 cgcaggtatt attaagtgcg ccgaccgggg ccgggaaatc aacctggctg ccgctgcaac
     5101 tgctggcgca tcccggcatt aacgggaaaa ttatcctgct ggagccgcgt cgtctggcgg
     5161 cgcgtaacgt cgcgcaacgg ctggaggagc tgcttaacga aaagccaggc gataccgttg
     5221 gctaccggat gcgtgcgcaa aactgcgtcg ggccgaatac ccgcctggaa gtagttaccg
     5281 aaggcgtgct gacgcggatg atccagcgtg acccggagtt gagcggtgtt ggattggtga
     5341 tccttgatga atttcatgag cgtagcttgc aggcggattt ggcgttggcg ctgttactcg
     5401 atgtgcaaca aggtctgcgt gatgacctta aactgctaat tatgtcggct acgctggaca
     5461 acgaccgctt gcagcaaatg ctgccagaag cgcccgtcgt catctcagaa gggcgctcgt
     5521 ttccggttga acgccgttat ttaccgctgc ccgcgcatca gcgttttgac gatgccgttg
     5581 cggtagccac cgctgaaatg ctgcgtcagg aaagcggatc attactgtta tttttacctg
     5641 gcgtcggaga aattcagcgt gtgcaggaac aactggcttc gcgcatcggc agtgatgtat
     5701 tgctctgccc gctgtatggc gcgttgtcgc tgaacgatca gcgaaaagcg atcctcccgg
     5761 caccgcaagg gatgcgcaaa gtggtgctgg cgaccaatat tgctgaaacc agtttaacca
     5821 tcgaaggtat tcgtctggtg gtggattgtg cccaggagcg tgtggcgcgt tttgatccgc
     5881 gcacggggct aacgcgactg attactcaac gcgttagcca ggcatccatg acgcagcgtg
     5941 ccgggcgcgc cgggcgtctg gagccgggta tctgcctgca tctaatcgcc aaagaacaag
     6001 cagaacgcgc tgccgcgcaa agtgaaccgg aaatcttaca aagcgatctt tccggtttgc
     6061 tgatggaatt actgcaatgg ggatgcagcg atccggcgca gatgagctgg ctggatcaac
     6121 cgccaacggt gaatctactg gccgcgaaac gcctgttaca aatgctgggg gcgctggagg
     6181 gtgaacggct tagtgcgcag gggcaaaaaa tggcagcgct gggtaacgat ccgcgtttag
     6241 cggcaatgct ggttagcgcg aagaacgacg acgaagctgc taccgcggca aaaattgccg
     6301 ccattctcga agagccgcca cggatgggca atagtgacct gggcgtggcg ttttcgcgca
     6361 atcaaccagc ctggcagcaa cgtagtcagc aactgttaaa acgcttaaac gtacgtggcg
     6421 gtgaggcaga cagttcgctt atcgcgccgc tacttgccgg ggcgtttgcc gatcgcattg
     6481 ctcgtcgccg tgggcaagat ggacgctatc aactggcaaa cggcatggga gcgatgctcg
     6541 atgccgacga cgcgctaagc cgccacgaat ggttgatcgc accgttatta ttgcagggca
     6601 gcgcctcgcc ggatgcgcgg attttactgg cgctgccggt cgatattgat gagttagtac
     6661 aacgctgccc gcagctggta cagcagtctg acactgtgga gtgggatgac gcgcaaggta
     6721 cgctgaaagc ctggcgtcgg ctgcaaatcg gtctgctgac ggtgaaagtg cagccgctgg
     6781 cgaaaccctc ggaagacgag ttgcatcagg cgatgcttaa tggcatccgt gataaaggtt
     6841 taagcgtgct caactggacg gcggaagcgg aacagctacg cttgcgtttg ttatgcgccg
     6901 caaagtggtt gccggaatat gactggccag cggttgatga tgaaagttta ttggcgacgc
     6961 tggaaacgtg gctgctgcca catatgactg gcgtacattc actacgcggc ctgaaatcac
     7021 tcgacattta tcaggcacta cgcggattac ttgattgggg aatgcagcaa cgtctggata
     7081 gtgaattgcc tgcgcattac actgtgccga cgggaagccg gatcgccatt cgttatcatg
     7141 aagataaccc gcccgcgctg gcggtgagaa tgcaagagat gtttggcgag gccaccaatc
     7201 cgacgatcgc ccaggggcgc gtgccgctgg tgctggagtt gctttcacct gcccaaagtc
     7261 cattacaaat cacacgagat ttgagcgcct tctggaaagg agcgtaccgt gaggtgcaaa
     7321 aagagatgaa agggcgttat cccaaacatg tctggccgga cgacccggca aatactgcac
     7381 cgacgcgacg gacgaaaaag tattcgtaag gttggttttc tccctctcc
//
LOCUS       DAZPEB010000184         7409 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZPEB010000184 DAZPEB010000000
VERSION     DAZPEB010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7409
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.188"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            1..1459
                     /gene="lpfC-O113"
                     /locus_tag="ACHURJ_003487"
                     /pseudo
     CDS             1..1459
                     /gene="lpfC-O113"
                     /locus_tag="ACHURJ_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
     gene            1470..2543
                     /gene="lpfD-O113"
                     /locus_tag="ACHURJ_003488"
     CDS             1470..2543
                     /gene="lpfD-O113"
                     /locus_tag="ACHURJ_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="LpfA-O113 is the major subunit of a form of long
                     polar fimbria first described in LEE-negative, Shiga
                     toxin-producing enterohemorrhagic Escherichia coli (STEC)
                     of serotype O113:H21. This protein, designated LpfD-O113,
                     is the minor subunit of this chaperone-usher type fimbrial
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial minor subunit LpfD-O113"
                     /protein_id="HFR1282293.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGAKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMANVYIRGDITAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVITHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKLGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            2791..3831
                     /gene="pstS"
                     /locus_tag="ACHURJ_003489"
     CDS             2791..3831
                     /gene="pstS"
                     /locus_tag="ACHURJ_003489"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFR1282294.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYDYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            3918..4877
                     /gene="pstC"
                     /locus_tag="ACHURJ_003490"
     CDS             3918..4877
                     /gene="pstC"
                     /locus_tag="ACHURJ_003490"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFR1282295.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            4877..5767
                     /gene="pstA"
                     /locus_tag="ACHURJ_003491"
     CDS             4877..5767
                     /gene="pstA"
                     /locus_tag="ACHURJ_003491"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFR1282296.1"
                     /translation="MAMVEIQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            5858..6631
                     /gene="pstB"
                     /locus_tag="ACHURJ_003492"
     CDS             5858..6631
                     /gene="pstB"
                     /locus_tag="ACHURJ_003492"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFR1282297.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            6646..7371
                     /gene="phoU"
                     /locus_tag="ACHURJ_003493"
     CDS             6646..7371
                     /gene="phoU"
                     /locus_tag="ACHURJ_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFR1282298.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
BASE COUNT         1864 a         1717 c         1933 g         1895 t
ORIGIN      
        1 cagatgttga tgtcagcgtg ggtgaattgc gcgatgaaga cggatttaca ccggacgtcc
       61 ttcaggcgca aatacttcat ggtttttccc acgggatcac tttatatgga ggtatgcagg
      121 ctgctgaaaa ttatggttct gcagctctgg gtgtcggtaa agatcttggc gctttgggcg
      181 caatttcttt cgatgtgaca catgctcgtg cgaattttag ccatgatgat acagaaacgg
      241 gtcagtcata tcgctttctc tattcaaaac gatttgacga cacagacact agcttgcgcc
      301 tggttggcta tcgttactcc accgagggct actataccct caatgagtgg gcatcgcggc
      361 gcaacagccc tgaagacttt tgggaaacag gtaaccgacg tagtcgcgtg gagggaacgc
      421 taacgcagtc gttggggaga gattatggca atttatacct gacattaagc cggcaacaat
      481 actggcatac cgatgatgtc gaacgattaa tgcaatttgg ctacagcagt agctggaagc
      541 gtctctcgtg gaacgtctcc tggagttatt ccaatactgc cagacagggg acggggaaca
      601 accatgccag tgataacacc agtgagcaga tctacatgct ctctttatct gttcctttat
      661 cgggctggtg gggtaatagt tacgccacct attctgtttc gcaaaacgat aattccggta
      721 gctcacatca agcggtacgg cgctggaaag aaataacctt tcatggaatt taatgcagtc
      781 ctataacagt catgatgatg aggttggcgg taatatgtcc ctgacctatg atggctctta
      841 tggcacggtg aacggcagct ataactacag ccaaaattcc cagaggctga attatggtat
      901 cagaggggga attctggcac acagcgaagg ggtaacgtta agtcaggagt taggtgaaac
      961 tattgctctt gttaaagcac ctggggccgc cgggttagaa atagataata tgcgcggtgc
     1021 tgcgacggac tggcgcggct atacggtcaa gacacagcta aacccttatg atgaaaatcg
     1081 ggtagcaatc agcgataact atttctcgaa gtcgaatata gaacttgata ataccgtcgt
     1141 tacgatggtt cccacgcgtg gtgcagtggt taaagcggag tttgtgactc atgtgggtta
     1201 tcgcgttctc ttcagagtgt taaatgcaaa tggtaaaccg gtaccttttg gagccattgc
     1261 tgcgatacaa gatgcaagtt tggcagattc aggaattgtc ggtgaccgtg gcgaacttta
     1321 tctttctggt ctaccagaaa aaggacaggt tacgttatcc tggggagaaa acgcctcaac
     1381 aaaatgcatc ttcaattatt cactttcgac accagaaagt gagagcggat taattgaaca
     1441 gggtgtgaca tgtcattaaa aggattttta tgaacaagta tatcaaacag tggtgctttg
     1501 ctgtgtttat gctctcgtta agtagtgtag cacttgcagc tcctaaaggt atctgtaccc
     1561 ctgataatgg cgtatttcac agtacgcttg atttctcggg atatcttatt accgcgaacg
     1621 aaaataaggt tggaacgaca tttaacacca ctgtaactaa tggatcctca tatcctggac
     1681 gctgtcattg tgatactggt aacgtaggag aattccccta tatctattat acgtcaaaaa
     1741 taaaccaggc attaacttat gctggggttc actctaatat taattattat gatcttaatc
     1801 cgaaccttga tgttgggatt gctatagata ttcttggggt tgggtatgtg aatgcacctt
     1861 ttgaatatca tgctaacaac ccgtctggta atacaaagta caattgcaac cgcattgaac
     1921 ctttaagtat atctagtggg gctaaagcga tagtatattt ttatatcaag aagacatttg
     1981 caggaaaatt gattattcct gaaacaaaga tagtgacatt gtatggaaca attagccgtg
     2041 acaccccggt ggattactca cagccgatgg ctaatgttta tattcggggc gatattacag
     2101 ctccgcaaag ttgcgaaata aataatttac agccagttta ttttgatttt aaagaaatac
     2161 cagctgcaga tttttcatct gttgttggaa gtgcggtaat aacacataaa attactaaaa
     2221 cggtcactat tgagtgtgaa aacctgggga tactgaatac cgatgatatc agtacgtctt
     2281 tttatgctac cgaacccaat acggacaatt caatggtcgt aacttcaaac tctaacgtgg
     2341 ggataaaaat ttacgacaaa aataataagg aaatcaaagt gaacggtggt gagttgccaa
     2401 cagacatggg taaatcaaca gtgtatggtg aaaaattagg tagtgtaact ttttcagctg
     2461 ctcctgcgag ccttactgga gctcgcccag ccccggggca atttactgca acagcgacga
     2521 taactgttga gattgtgcgc taataggtct gacaacgatt gttcatcaca tcgttgtcat
     2581 ctcacccatt tctttatcac ttttaaatgc atcatgacaa aaagatgaca tgtgggattt
     2641 tcatcttcac atttaaaatc agtaagttat ttggtaattc ctttctgtca taaaaatgtc
     2701 atattcctta catataactg tcacctgttt gtcctatttt gcttctcgta gccaacaaac
     2761 aatgctttat gaatcctccc aggagacatt atgaaagtta tgcgtaccac cgtcgcaact
     2821 gttgtcgccg cgaccttatc gatgagcgct ttctctgtgt ttgcagaagc aagcctgaca
     2881 ggtgcaggtg caaccttccc tgcgccggtg tatgccaaat gggctgacac ttaccagaaa
     2941 gaaaccggta ataaagttaa ctaccagggt atcggttctt ccggtggcgt aaaacagatt
     3001 atcgctaata ccgttgattt tggtgcctct gacgcgccgc tgtctgacga aaaactggct
     3061 caggaaggtc tgttccagtt cccgaccgtg attggcggcg tggtgctggc ggttaacatt
     3121 ccagggctga agtctggcga actggtgctg gatggtaaaa ccctcggcga catctacctg
     3181 ggcaaaatca agaagtggga tgatgaagcc atcgccaaac tgaatccggg tctgaaactg
     3241 ccttcacaaa acattgctgt agtacgccgc gcagatggct ccgggacttc cttcgtcttc
     3301 accagctacc tggcgaaagt gaacgaagag tggaaaaaca acgttggtac tggctctacc
     3361 gtaaaatggc cgatcggtct gggcggtaaa ggtaacgacg gtatcgccgc gttcgttcag
     3421 cgtctgccgg gtgcaattgg ttatgttgaa tatgattacg cgaagcagaa caacctggcg
     3481 tacaccaaac tgatctccgc tgatggtaaa ccggttagtc cgaccgaaga aaacttcgct
     3541 aatgcagcaa aaggtgcaga ctggagcaaa accttcgctc aggatctgac caaccagaaa
     3601 ggcgaagatg catggcctat tacctctacc acgttcattc tgatccacaa agatcagaag
     3661 aaaccagaac aaggcacaga agtgctaaaa ttcttcgact gggcgtacaa aaccggggct
     3721 aaacaggcga acgacctgga ttacgccagc ctgccggata gtgtagttga acaggttcgc
     3781 gctgcgtgga agaccaatat taaagacagt agcggtaagc cgctgtacta ataaaactcc
     3841 aggccgggta cggtgtttta cgccgcatcc ggcattacaa aatgactttg taaacgcgtt
     3901 taactgaaga gtaacttatg gctgcaacca agcctgcttt taacccaccg ggtaaaaagg
     3961 gcgacataat tttcagcgtg ctggtaaaac tggcggcgct gattgtgcta ttgatgttgg
     4021 gtggcattat tgtctctctg atcatctcct cctggccgag cattcagaaa tttggtctgg
     4081 ctttcctatg gaccaaagag tgggatgcac cgaacgatat ctacggggcg ctggtgccga
     4141 tctacggtac gttggtgact tcgtttatcg cgctgctgat cgccgtcccg gtgagtttcg
     4201 gtatcgccct gttcctgact gagcttgcgc ctggctggct gaaacgcccg ctgggtatcg
     4261 ccattgagct gctggcagcc attccaagta tcgtttacgg catgtggggc ctgtttatct
     4321 ttgcgccgct gttcgccgtt tactttcagg agccggtcgg caatatcatg tcgaatatcc
     4381 cgattgttgg cgcgctgttc tctggccccg catttggtat cggtatcctc gcggcaggcg
     4441 tgatcctcgc catcatgatt attccgtaca ttgcggcggt aatgcgtgat gtgttcgaac
     4501 aaaccccggt gatgatgaaa gagtcggcct acggtattgg ctgcaccacc tgggaagtta
     4561 tctggcgtat cgttcttccg ttcaccaaaa atggtgttat cggcggcatc atgctggggc
     4621 tgggccgcgc gctcggtgaa accatggcgg tgacctttat catcggtaac acctaccagc
     4681 tcgacagcgc ctcgctgtac atgccgggca atagtatcac ctctgcgttg gcgaacgaat
     4741 ttgcggaagc ggaatccggt ctgcacgttg ccgcactgat ggaactgggc ctgatcctgt
     4801 ttgtgattac cttcatcgtc ctcgccgcat cgaagtttat gattatgcgc ctggctaaga
     4861 atgagggggc acgctaatgg ctatggttga aatccaaacc actgcggcgc tggctgaatc
     4921 tcgccgcaaa atgcaggcgc gtcgccgcct caaaaaccgt attgcgctga cgctctcgat
     4981 ggcgacgatg gccttcggcc tgttctggct gatctggatt ttaatgtcca ccatcactcg
     5041 cggtatcgac ggtatgtcgc tggcgctgtt cactgaaatg acgccgccgc ccaatacgga
     5101 aggtggtggt ctggcgaacg ccctggcggg tagcgggctg ttaattttgt gggccacggt
     5161 attcggtacg ccgctgggca ttatggcggg gatttatctg gcggaatatg gtcgtaaatc
     5221 ctggctggca gaagtgattc gcttcattaa cgacattctg ctctccgcgc cgtcgattgt
     5281 ggttggtctg tttgtttaca ccatcgtggt ggcgcagatg gagcacttct ctggctgggc
     5341 gggcgtgatt gccctggcgc tgttgcaggt gccgattgtt atccgcacca ccgagaacat
     5401 gctgaaactg gtgccggaca gcctgcgtga agcggcttat gcgctgggta caccgaagtg
     5461 gaagatgatc tctgcgatta cgctgaaagc gtcggtgtcc gggatcatga ccggtatcct
     5521 gctggcgatt gcccgtattg ctggtgaaac cgcgccgctg ctgtttaccg cgctctccaa
     5581 ccagttctgg agcacggaca tgatgcagcc gatcgccaac ctgccggtga cgatctttaa
     5641 gtttgcgatg agcccgtttg cggaatggca acaactggcc tgggccgggg tattgattat
     5701 taccctgtgc gtactgctgc tgaacattct agcgcgcgtt gtttttgcga agaataaaca
     5761 cggttaacac tttgccggat gcggcgtgaa cgccttatcc ggcctacggt aagcctgatt
     5821 agcgaagtgc atcaggcacg atgaggaaaa gattgcaatg agtatggttg aaactgcccc
     5881 gagtaaaatt caggttcgta atttgaactt ctactacggc aaattccatg ccctgaaaaa
     5941 catcaacctg gatatcgcta aaaaccaggt tacggcgttt atcgggccgt ccggctgcgg
     6001 taaatcgacg ctgctgcgta ccttcaacaa aatgtttgaa ctgtacccgg agcagcgtgc
     6061 ggaaggtgaa attctgcttg atggcgacaa catcctgacc aactctcagg atatcgcact
     6121 gctgcgtgcg aaagtgggca tggtgttcca gaaaccgacg ccgtttccga tgtccatcta
     6181 cgacaacatc gcttttggcg ttcgtctgtt tgagaagctc tcccgtgccg acatggacga
     6241 gcgcgtgcag tgggcattga ccaaagccgc attgtggaac gaaaccaaag ataaattgca
     6301 ccagagcggt tactctctct ctggtggtca gcaacagcgt ctgtgtattg cgcgtggtat
     6361 cgccattcgc ccggaagtgc tgctgctcga cgaaccgtgt tcggcgctcg accctatctc
     6421 taccgggcgt attgaagagc tgatcaccga actgaagcag gattacaccg tggtgatcgt
     6481 cacccacaac atgcagcagg ctgcgcgttg ttccgaccac acggcgttta tgtacctggg
     6541 cgaattgatt gagttcagca acacggacga tctgttcacc aagccagcga agaaacaaac
     6601 agaagactac atcaccggtc gttacggttg attcaggagt gcgttatgga cagtctcaat
     6661 cttaataaac atatttccgg ccagttcaac gccgaactgg aaagtatccg cacgcaggtg
     6721 atgaccatgg gcggcatggt ggagcagcag ctttctgatg caatcaccgc gatgcataac
     6781 caggacagcg atctggcgaa gcgcgtgatc gaaggcgaca agaacgtcaa catgatggaa
     6841 gtggcgatcg atgaagcctg cgtgcgcatt atcgccaaac gtcagccgac ggcgagcgac
     6901 ctgcgactgg ttatggtgat cagtaaaacc attgccgagc tggagcgtat tggcgacgtg
     6961 gcggacaaaa tctgccgtac tgcgctggag aaattctccc agcagcatca gccgttgctg
     7021 gtaagtctgg agtcgctggg ccgtcatacc atccagatgc tgcacgacgt gctggacgcg
     7081 ttcgcgcgga tggacattga cgaagcggta cgtatttatc gtgaagataa aaaagtcgat
     7141 caggaatacg aaggtattgt tcgtcaactg atgacctaca tgatggaaga ttcgcgtacc
     7201 attccgagcg tacttactgc gctgttctgc gcgcgttcta tcgaacgtat tggcgaccgc
     7261 tgccagaata tttgtgagtt tatcttctac tacgtgaagg ggcaggattt ccgtcatgta
     7321 ggcggtgatg agctggataa actgctggcg ggaaaagata gcgacaaata attcaccaga
     7381 caaatcccaa taacttaatt attgggatt
//
LOCUS       DAZPEB010000185         7319 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZPEB010000185 DAZPEB010000000
VERSION     DAZPEB010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7319
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.189"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            501..1070
                     /locus_tag="ACHURJ_003494"
     CDS             501..1070
                     /locus_tag="ACHURJ_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFR1282299.1"
                     /translation="MTYKYNPFWQQRIRETVRHALDVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPESLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLARNDDTGFQ
                     QVLERADYLAKEHTKAHCTGERNFGCSRS"
     gene            1330..1731
                     /locus_tag="ACHURJ_003495"
     CDS             1330..1731
                     /locus_tag="ACHURJ_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFR1282300.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYANYTCHLCGSALRYHPQY
                     DTELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGGFSIPRTTQEEICEF"
     gene            1719..2153
                     /locus_tag="ACHURJ_003496"
     CDS             1719..2153
                     /locus_tag="ACHURJ_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFR1282301.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDENYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
     gene            2508..2888
                     /locus_tag="ACHURJ_003497"
     CDS             2508..2888
                     /locus_tag="ACHURJ_003497"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR1282302.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQQLVAATCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEVTFR
                     HGTLRFNGNVSEKLLTLLIQELKR"
     gene            2885..3232
                     /gene="tnpB"
                     /locus_tag="ACHURJ_003498"
     CDS             2885..3232
                     /gene="tnpB"
                     /locus_tag="ACHURJ_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR1282303.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKAQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            3282..4667
                     /locus_tag="ACHURJ_003499"
                     /pseudo
     CDS             3282..4667
                     /locus_tag="ACHURJ_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(4906..6264)
                     /locus_tag="ACHURJ_003500"
     CDS             complement(4906..6264)
                     /locus_tag="ACHURJ_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HFR1282304.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNHG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
     gene            6546..6677
                     /locus_tag="ACHURJ_003501"
                     /pseudo
     CDS             6546..6677
                     /locus_tag="ACHURJ_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(7015..7296)
                     /locus_tag="ACHURJ_003502"
     CDS             complement(7015..7296)
                     /locus_tag="ACHURJ_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071468 - cellular response to acidic pH
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development regulator YmgB/AriR family
                     protein"
                     /protein_id="HFR1282305.1"
                     /translation="MTEPWGRKMHSDYYNMVFGEKLANILYEANSQFFYERNVIEEAV
                     NALFCEREIINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
BASE COUNT         1747 a         1917 c         1916 g         1739 t
ORIGIN      
        1 ttgaggggct gtgagtttcg cgtatttgtc agaaacccag atgagttttc ctgtatggga
       61 cgaaataagg cagcggcaat acctggaaaa tttaatatgg tatgaaggag aacctgtcgg
      121 ttacgcttaa tatacacggt acattgtcag ctcaggggat acatgaaccg ttatctcacc
      181 agctacacag tatacacaca ctgacgctga cagtagtcag caggaccaca atacgggcaa
      241 cacactgtat tatgagtaat cgcgtttcca cagtccctgc acttcttttt gcgtatcacc
      301 atcactccct cctcacagcc ttacccgtaa ccggtttttt acattaaaaa acaacgctga
      361 aaattcaacc gtcagtccgg agacgaccgt tcgggttatc acagagtgcc tgagacagtg
      421 tcctaccgga ggtcacttca gggactctgc gtatttttta cgacgtggct attccgtatg
      481 aaccatacgg agatttaacc atgacctaca aatacaaccc cttctggcag caacgtattc
      541 gtgagacggt gcggcacgca ctggatgttc atccccgcct gacggcactt cgggttgatc
      601 tgcgtctccc ggatgtgccg gcagcaacgg acgcagctgt gatatcccgc ttcatcaatg
      661 ccctgaaagc ccgaatcgac gcttaccaga aacgtaagca tcgggaaggt aaacgcgtgc
      721 atcccacaac cctgcattac gtctgggccc gggagtttgg ggagtgcaaa ggtaaaaaac
      781 actatcacct gatgctgctg gtcaaccggg atacctggtg tcgtgccggt gattaccgcg
      841 ctccggagtc tctcgccggg atgattaaac aggcgtggtg cagtgccctg ggagtggatg
      901 tcgggtgcca tgccacgctg gtgcattttc cggcctggcc ggcggtgtgg ctggcgcgta
      961 atgatgacac cggctttcag caagtgctgg aacgtgctga ctatctggcg aaggagcata
     1021 ccaaagctca ctgcaccggt gagcgcaatt ttggctgtag ccggagttga gcacgactgg
     1081 cgcgccaata tccggcaaac caaaagtatg ggttgttgtc tggcggcaac gatgtatcca
     1141 ctcgcccact gactgttttt tacgtcagtg ttccattgtg tgacaaccac agtgctgtaa
     1201 cagcaaccag cctgctttac ccaaactcca gtccagattt taactgaacc gccattcaca
     1261 ccctgaccac gctgcccgta ccgggacggc tgttgcctgc gtgtcgtctg gcggctgagg
     1321 tatatgacta tgtacgcaaa atcctttatc gctcttgatg gcaacggacg tctgacgggc
     1381 gcccgtactg cacaggccgc accttatgct aactacacct gccacttgtg tggcagtgca
     1441 ctcagatacc atccgcaata cgacactgaa cttccctggt ttgaacacac tgacgacagg
     1501 ctgacagagc acggtcaaca gtgcccttat gtcaggccgg agcgcagaga aatacagttg
     1561 attaaacgtc tgcagcaatt cgtaccggat gccttacccg tggtgcgtaa agccagctgg
     1621 cactgcagac aatgtcacca cgattattat ggggagcagt actgcacaca ctgccagacc
     1681 ggaggattca gtattccccg gacaactcag gaggaaatat gcgaattctg aactgttata
     1741 tggcgaatga cagcaaaggc cattttgtta cggcgaaaga agctgcgaag cacaaccgac
     1801 aggacgtttt gtgctgtgtg tcctgtggat gcccgttaac acttcagcgg ggcaatgacg
     1861 gacaaccacc gtggtttgaa catgaccaga tgactgtcgc tgaaaaaatc ctgctgcgat
     1921 gtacctggct tgacccggca gagaaagagg cccgtcgttt gcatctgcag ggcatgacgg
     1981 ttccggatta tacggtgaag gtgagaaagt ggttttgtgt gatgtgtgac gaaaattatg
     2041 agggggaaaa gtgctgccca cgctgcggta ccggggtata cagcagggcg tgggggcggc
     2101 aggaggtgcc gtcggaagat gccagggctg ataatccgtt acagaggctg taatggttgc
     2161 ctccggagca gtttgcggtg atgcctttcc cgggaatgga ttgccagtgc ggggactgtg
     2221 gagcttaatt ccggtgttgg tggcctttcc gttgatttta tgccaacagc cccctgtgac
     2281 tgacaggctg cctcgtcatt ccattcgtat ctccagtaac agggggttgt attgtatttt
     2341 attgtgccgg ttcaggtgtg cgtttcccgg cgcgtctgca ccggcttaac caaattcaac
     2401 agggataaaa tagtggtaag cgtacagcct gaaccgtctg gtcagaatct gacgaattag
     2461 acaaagtggt gtccaccaaa taagtagtgg gaaccaaagt atcagatatg cagaaaaatg
     2521 tgactcccgg caggcgaaaa ggctgcccta attatcctcc cgaatttaaa cagcagctcg
     2581 ttgctgccac ctgtgaaccc gggatatcca tctcaaaact tgctcttgaa aatggcatta
     2641 acgccaatct gttgttcaaa tggcgacaac aatggcgcga gggaaagctg ctattacctt
     2701 cttcagagag cccccagcta cttcctgtga ctctcgatgc cgctgccgaa cagccagaat
     2761 cgctcgcaga ggacccggaa accctcagta tcagctgtga ggtaacgttc cggcacggga
     2821 cgctccgctt caatggcaat gtcagcgaaa agctcctgac tctactgata caggaactga
     2881 agcgatgatc ccgttacctt ccgggaccaa aatttggctg gttgccggta tcaccgatat
     2941 gagaaatggc ttcaacggcc tggctgcgaa agcacaaacg gcgctgaaag acgatcccat
     3001 gtccggccat gttttcattt tccggggccg cagcggcagt caggttaaac tgctgtggtc
     3061 caccggtgac ggactgtgcc tcctgaccaa acggctggag cgtgggcgct tcgcctggcc
     3121 gtcagcccgt gatggcaaag tgttccttac gcaggcgcag ctggcgatgc tgctggaagg
     3181 tatcgactgg cgacagccta agcggctgct gacctccctg accatgctgt aaatctcttt
     3241 atcctggttg tcacagaata agcccggtaa aatacgggct tatgaacgac atctcttctg
     3301 acgacatctt cctgctgaaa cagcgcctgg ccgaacagga agcgctgatc cacgccctgc
     3361 aggaaaagct gagcaaccgg gagcgcgaaa tagaccatct gcaggcgcag ctggataaac
     3421 tccgccggat gaacttcggc agtcgttccg aaaaagtctc ccgccgtatc gcacaaatgg
     3481 aagccgatct gaaccgactt cagaaagaga gcgatacgct gactggtagg gtgtatgacc
     3541 cggcagtaca gcgtccgttg cgtcagaccc gcacccgtaa gccgttccct gaatcactac
     3601 cccgtgacga aaagcgactg ttgcctgcgg cgccgtgctg cccgaactgc ggcggttcac
     3661 tgagctatct gggcgaggat accgccgaac agctggagtt gatgcgtagc gccttccggg
     3721 ttatccggac ggtacaggaa aaacatgcct gtactcagtg cgataccatc gtgcaggcac
     3781 ctgcaccttc gcggcccatc gagcggggta tcgccggacc ggggctgctg gcccgcgtgc
     3841 tgacctcgaa gtatgcagag cacaccccgc tgtatcgcca gtcagaaata tacggccggc
     3901 aaggtgtgga gctgagccgt tcactgctgt cgggctgggt ggatgcatgc tgccggctgc
     3961 tgtctccgct ggaagaggcg cttcatggct atgtcatgac tgacggcaaa ctccatgccg
     4021 atgatacccc ggtccaggta ctgctgccgg gtaataagaa gacgaagacc gggcggttgt
     4081 gggcgtatgt tcgtgatgac cgcaatgccg ggtcagcgtt ggcacctgca gtgaggttcg
     4141 cttacagccc ggacagaaaa ggcatccatc cgcagactca tcttgcttgc ttcagcggtg
     4201 tgctgcaagc ggatgcgtac gccgggttca acgagctgta tcgcaatggt gggataacgg
     4261 aagctgcctg ctgggctcat gcccgctgaa agatccacga tgtgcacgtc cgcatcccgt
     4321 cagcactgac ggaagaagcc ctggagcaga tcggtcagtt gtacgccata gaggcggata
     4381 taaggggaat gccggcagag cagcggcttg ctgaacgtca gcgaaaaacg aaaccgctgt
     4441 tgaaatccct ggaaagctgg ttgcgtgaaa agatgaagac cctgttcttc ggctctggcc
     4501 atggtggtga gcggggagcg ctactgtaca gcctgatcgg gacgtgcaaa ctgaatgacg
     4561 tggatccaga aagctacctt cgccatgtgc ttggcgtcat agcagactgg ccggtcaacc
     4621 gggtcagcga actgcttccg tggcgcatag cactgccagc tgaataacac atccccgtca
     4681 atacggccct cgctgtacgc ttacttttgt tcggactgct tatggccttt ttccactggt
     4741 gtagcaggaa agttaaccgg ctgccaccat gggatgacgt gtaaaccgtg caggcatgtt
     4801 ttcacgtggt gacgctttca ttgagggtct acgccggaac cccaatattg ttcacataca
     4861 tgaatttgaa aaactaagcg atactgctgt ccgtacctta taaacttatt tcaacgcttc
     4921 cggcagcgtc acaatccaca gttcactgtc agactccggt ttctccagtg gcttcactct
     4981 gagggtcgtt tcaacctgct tgtcgtcaag cgtcagcttt ccttccgcgt tcacccgata
     5041 atccttgaat gtcttgccct cgaccggatg ttgcatcgcc accttcacgc caaagtcgtt
     5101 atcgttcgcc accgccagcg ttttgctgtc gatcagcgcc aacccttcag ccttctcctg
     5161 ctgccagccc aaggagcgca gatcaacaac ctgcgttttc tgcgcaaggg taatgccgcg
     5221 ctgtgacagg gttttctcat catcaaactc cggatactca cccggcttgt cgaagcctga
     5281 caggtcgctc gccttattga gatcgacctc gtaaatcagg ttacgcattc tgttgttttt
     5341 atctcttccc tgttcgatca gcaggatgtg ctggttatcg agcgccacga tgtctccgat
     5401 tttagcgtcg ctgtttttgc tgtaggccgc gctgtcgata ggataaccgt acatcgcggt
     5461 ttttccgctt gccggatcga agctcaccag acgcgtaaac agcgcctttt tcttgctctt
     5521 agcgtcgata tccagcgtac tttgcacggc gacaataata cgcccgtccg gcatacgggt
     5581 gaggccttca aagccgcggt tcgcctgacg ccatttgagg atatttggca aaccgcccgc
     5641 aatcgctttt tccccttccg ccgcctgcgg accgtggatt gccaggattt tccctttact
     5701 gtcgatgtta atcaggaacg ggccatactc atcgcacagc cagtaacctc ctttaccatc
     5761 cggagtgatc ccttccgtat ccagcccgtg attatccccc ttcagtctat gtaacgtgtc
     5821 gctgaaggcc acttcattgg tggagccaat cacgtcgctt gccagcggca gaccgttgat
     5881 cgcaccttta tcgtcatgca gaggacgggg atcgatggcc tccgctttgc cgttttgtac
     5941 acgaatcgtc atcagcagcg gcgcgaaatc tggcgtaacg aaaattttag tttcgttttt
     6001 cccctctttc ggtgaatccg cgttcggacc gcgatcggta atggttgcga atgtcagggc
     6061 atcgccctgt ttaccggtga acagcaggcc agaacctatc ccaaccggaa ggccgttcgg
     6121 gaatgcactg gcaaatgcgc cggcgtagtt cacgtgcgtg ccttctggaa aactgacgac
     6181 atagcgctca gccgtaggtt gtgccgccag ggcagagaaa gagagcgtac aaccgattag
     6241 cacgggaata atttttcttt tcatattgtt agcatccata aagtgaagat cacactacac
     6301 cataaagaag aaacgagtca ggaaaataac atcgaatggc aacatgcgcg ggcctgaggc
     6361 caaagaaatg taaaacactg ttgttatttg taacaacgga aacatttacg gaaattattt
     6421 gtctttcttc gtgatcaaaa atgctgtgtc ttgttggatt ggagtgatga gggctgcgtt
     6481 taggaaacgc cctatattgt acggttgcat tatcgcggta ggggtaatgc acgtgtcagt
     6541 atgatgagct gaccacactc acgtgggtgg actggtataa caatcgacga ttgctggaaa
     6601 ggcagggcca tatcccaccg gcagaagcag aaaaagctta ttatgcttcc atcggaaacg
     6661 atgatctggc agcctgaatt cacagataaa acactctcca ggaaacccgg ggcggttcac
     6721 ttagccgcgt gtccattatt gctggataag atcataacga tacaccgggt taaacaggtg
     6781 gtcactgctt cactgaagct gcgtgattat gatgctcagg tagcagaagc tatgcccatg
     6841 ataccaaaat gacgaaagcc gaaatgtctg aaagcgtgtg tattacctga aaatatgacc
     6901 agctccgggg tacgtaaccg attcaacaaa gccaatcgat attacaaaac gagagggact
     6961 atatatttcg ttttcagata agactccttg agagattatc ttttatgtaa aatctcaaat
     7021 atcacctgat gtaatgtcaa tgataatatt aagtgcgctc tgaacaacat ctttcttggt
     7081 gtgattaaca tctgacaaaa agaacatcag cttctttatt atgttcttgt tgtttattat
     7141 ttcacgctca cagaataaag cgttgactgc ctcttctatg acatttctct cgtaaaaaaa
     7201 ttgagagtta gcttcatata aaatattggc caatttttca ccaaaaacca tattataata
     7261 atcactgtgc atctttctcc cccagggttc agtcaataat tttaccatgc ctaattatg
//
LOCUS       DAZPEB010000186         7284 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZPEB010000186 DAZPEB010000000
VERSION     DAZPEB010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7284
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.190"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(330..1466)
                     /locus_tag="ACHURJ_003503"
     CDS             complement(330..1466)
                     /locus_tag="ACHURJ_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR1282306.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGGLQSLTVVPGTDGVQGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(1469..1831)
                     /locus_tag="ACHURJ_003504"
     CDS             complement(1469..1831)
                     /locus_tag="ACHURJ_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFR1282307.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIASLSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            2368..4281
                     /gene="mtlA"
                     /locus_tag="ACHURJ_003505"
     CDS             2368..4281
                     /gene="mtlA"
                     /locus_tag="ACHURJ_003505"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFR1282308.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLT
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            4511..5659
                     /gene="mtlD"
                     /locus_tag="ACHURJ_003506"
     CDS             4511..5659
                     /gene="mtlD"
                     /locus_tag="ACHURJ_003506"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFR1282309.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPSMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            5659..6246
                     /gene="mtlR"
                     /locus_tag="ACHURJ_003507"
     CDS             5659..6246
                     /gene="mtlR"
                     /locus_tag="ACHURJ_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFR1282310.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(6258..6467)
                     /gene="yibT"
                     /locus_tag="ACHURJ_003508"
     CDS             complement(6258..6467)
                     /gene="yibT"
                     /locus_tag="ACHURJ_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFR1282311.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            6752..7114
                     /locus_tag="ACHURJ_003509"
     CDS             6752..7114
                     /locus_tag="ACHURJ_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFR1282312.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT         1849 a         1829 c         1847 g         1759 t
ORIGIN      
        1 attgtgatct atagattaaa tattttttaa atgtgactgt catccttcag ataataaagc
       61 catcatcgca atagatggaa aagcactcct gctctttttt gattagagcc gtcgcaggag
      121 gaacaactca tatcatcatt gcgatctcga cgatgtatag tctcatcctg cggcagaaca
      181 agacggataa aaaatccaat gagagcacac tcatccagaa cttattaaca taatgaatat
      241 taaagaaaaa tcatcgcagc taatgaagta atatgcaaaa ataattagtc cttacaagga
      301 gcaaagttcc ataatctccc ccctccccct caatgatcca aataaagata atgcatccag
      361 ctggtcattc ttagcagcac tttccgcatc accgaaacat ggctgaaatg gtcggagtaa
      421 accgccacct gggcgtagat gccgtcgggt aaggcatcga tatcatcgtt agggtccagt
      481 tcaatggttc cctgcacacc gtccgtgccg ggcacgaccg ttaatgattg caatcccccc
      541 tgcgcctgat aagaaccgcc tggcacgaca ggtaaaatac tagtcagttt gccgtgaaac
      601 acctgcccag gtagcgcgtt aaacaccact tccgcatcat cgccgggttt cagacgtaac
      661 agcgagtttt gccgaaattg ggcgacaatt tgccgttttt gctcggggat gaagaccatc
      721 accggacgca gcggcaaggc agctgcgtat gtacctgggc ggatcagtac ctgagtaacg
      781 tagccattgc tcggcgcgcg aatgacagtc tgctcaaggt tatattttgc ttcagtaagt
      841 tgcgctctta agctcacaat ctgcgattgc tcgccgttaa ccatactgtc gagctggctc
      901 tggatctgcg cctgctccgc caccgagcct ttcaccagcg catcctgcgc gaggaaattt
      961 tgccgcgcat cgtcgatgtc acgttccgag aacggattca ccgccgcctg gctgcctttc
     1021 aagtaacgtt gataattttt aaacagacgg tcgcgctccg ctgaaacctg ggtggtgttg
     1081 gcctgcgctt cagtgagctg cgcacgcagc gtctttatat tatgcgtcgc cgtcatcagg
     1141 tcagcctgaa gtctgtcaac tcgcgcctgg taacgaaccg ggtcgagctt aaaaagcacc
     1201 tcgccctttt gaataagctg attattcttg tcagtgactt cagtaacaat tcccgtcacc
     1261 tgtggcgtga tagggatcgc tatcactgcc ttttgcgcgg taaaagtgta agggtggttg
     1321 tagttcatca acaaaatcaa accactcacc agaaacacgc ctcccagcgc cgccgtcgcc
     1381 agcgtccact gatttaccgg aatgcggaag attttaaaga ccgcccacgc cagcgccacg
     1441 taagttaaaa caatcaatag atccataatt agatctccgg gaacgtggaa tagtcagtct
     1501 ttttctcggc ggcgagttga tgctccagcc gggaaattcg gtcagaaagt gaggcgattt
     1561 ccgggtcagt cgctttctcc tgcgacgcaa catgtgactg cataccccag ccacgctccg
     1621 gttgatagag cgtcgcccag atccacagaa acggccagat aacatgcaga gtaaacaggc
     1681 tcacccagcc cgccgtatga atagcgtcgg catgggggtg attgcgcttt ttggcaatca
     1741 gataagggat gtcatgtatc gcgatgattc cataaaaaat caccagaaag acgaagatca
     1801 gcactcccaa cgcgaaatag tttagaaaca tatctgcctc ggattcacgt ttatcagtgt
     1861 tgtttttggg ctggcagcca gaagggagtc aggctgatat tttgacaata atccgggttc
     1921 gcgattctcg ccataacacc aaagaataat ttttagaggt gatgagttgc ttagttacat
     1981 aacgattgta tgacgaaggc ataacatgct gtagatcaca tcaggtgaac gccgtaagaa
     2041 aatatcttgt gattcagatc acaaagattc aacaaaccat caaaacaaaa atgtgatact
     2101 actcacattt aaatgccatt tttagcgaaa atcgccgcct tgttgctttt ttacacaagc
     2161 gttttgtgat gaacgtcacg tcaattacct ctctacctcc tatatttatg tgattaatat
     2221 cacacaaaag gccgtcgact ggacagttaa ccgattcagt gccagatttc gcagtatcta
     2281 caaggtccgg ctacctctgc cgccacatta acaaaaaacc tcgggcttcc agcctgcgcg
     2341 acagcaaaca taagaagggg tgtttttatg tcatccgata ttaagatcaa agtgcaaagc
     2401 tttggtcgtt tcctcagcaa catggtgatg ccaaatatcg gcgcgtttat cgcgtggggt
     2461 atcatcaccg cgttatttat tccaacaggg tggttaacga acgagacgct ggcgaagctg
     2521 gtcgggccga tgatcactta tctcctgccg ctgctgatcg gttataccgg tggtaagctg
     2581 gtaggcggcg aacgtggcgg cgtagtcggt gccatcacca ccatgggcgt tatcgtcggc
     2641 gcagacatgc cgatgttcct cggttctatg attgcaggtc cgctgggcgg ctggtgcatt
     2701 aagcacttcg accgctgggt agacggtaag atcaaatccg gttttgagat gctggtgaat
     2761 aacttctccg caggcatcat cgggatgatc ctcgctattc tggcattcct cggcattggc
     2821 ccgattgttg aagccctgtc caaaatgctg gctgcgggcg ttaacttcat ggttgtccat
     2881 gacatgctgc cgctggcgtc tatctttgtt gaaccggcga aaatcctgtt cctcaacaac
     2941 gccattaacc acggtatctt ctcgccgctg ggtattcagc agtcccatga actgggtaaa
     3001 tcaatcttct tcctgattga agctaaccca ggtccaggta tgggcgtgct gctggcgtac
     3061 atgttctttg gtcgtggtag cgctaaacag tctgcgggcg gtgcggcaat catccacttc
     3121 ctgggtggta tccacgaaat ctacttcccg tatgtgctga tgaatccgcg tctgatcctc
     3181 gcagtcatcc tcggcggtat gactggcgtg ttcacgctga ctatcctggg cggtggtctg
     3241 gtttctccgg catctccggg ttctatcctt gctgtactgg cgatgacacc aaaaggggct
     3301 tatttcgcta acatcgcggg tgtgtgtgcg gcgatggctg tctccttcgt tgtctctgct
     3361 attttgctga aaaccagcaa agtgaaagaa gaagatgata ttgaagcagc aactcgtcgt
     3421 atgcaggaca tgaaagctga gtctaaaggc acatctccgc tgtctgcggg cgatgtgact
     3481 aacgacctga gccacgtacg taaaatcatc gttgcctgtg atgccggtat gggttcaagt
     3541 gcgatgggcg caggcgtgct gcgtaagaaa attcaggatg caggtctgtc gcagatttcg
     3601 gtcactaaca gcgcgatcaa caacctgccg ccagatgtgg acctcgtcat cactcaccgt
     3661 gacctgaccg aacgcgctat gcgccaggtt ccgcaggcac agcatatttc gctgaccaac
     3721 ttcctcgaca gcggcctgta caccagccta accgaacgtc tggttgctgc ccagcgccat
     3781 actgaaaacg aagtgaaagt gaaagacagt ctgaaagaca gctttgacga ttccagtgct
     3841 aacctgttca aactgggcgc ggagaacatc ttcctcggtc gcaaagcagc aaccaaagaa
     3901 gaagcgattc gttttgctgg cgagcagctg gtgaaaggcg gttacgttga gccggaatac
     3961 gttcaggcga tgctggatcg tgaaaaactg accccgactt atctgggtga gtctatcgcg
     4021 gtgccacacg gtacggttga agcgaaagat cgcgtactga aaacgggcgt ggtgttctgc
     4081 cagtacccgg aaggcgtgcg cttcggtgaa gaggaagatg acattgcccg tctggtgatt
     4141 ggtattgcag cccgtaacaa cgagcacatt caggttatca ccagcctgac caatgcactg
     4201 gatgatgagt ccgtcatcga gcgtctggca cacaccacca gcgtggatga agtgctggaa
     4261 ctgttggcag gtcgtaagta atccaatccc accctctcca catggagaag gtggggttaa
     4321 ttgcctgatg cgctacgctt atcaggccta caggatgcat cacgatttgt tgaatttgca
     4381 cgttcttgta ggccggataa ggcgcttacg ccgcatccgg cgctgcccct ctcctcacgg
     4441 agagggtttg ggtgagggaa aagcctcacc ccagccctct cgggtaaaaa cattgatgaa
     4501 ggttaatact atgaaagcat tacattttgg cgcaggtaat atcggtcgtg gctttatcgg
     4561 taaactgctg gcagacgcgg gtatccaact gacgtttgcc gatgtcaatc aggtggtact
     4621 tgatgccctg aatgcccgtc atagctatca ggttcatgtg gttggtgaaa ccgagcaggt
     4681 agataccgtt tccggcgtca atgctgtcag cagcattggt gatgatgtcg ttgatctgat
     4741 tgctcaggtc gatttagtca ctaccgccgt tggcccggtt gtgctggaac gtattgctcc
     4801 ggctatcgcc aaagggctgg tgaaacgcaa agaacaaggt aatgaatccc cgctgaacat
     4861 catcgcctgt gaaaacatgg tacgcggtac cacgcagctg aaaggccatg tgatgaacgc
     4921 cctgccggaa gacgccaaag cgtgggtaga agaacacgtt ggctttgtcg attccgccgt
     4981 tgaccgcatc gtaccgcctt cggcttcggc aactaacgat ccgctggaag tgacggtaga
     5041 aactttcagc gaatggattg tcgataaaac gcagttcaaa ggcgcactgc cgaacatccc
     5101 aagcatggag ttaaccgaca acctgatggc atttgtcgaa cgtaaactct tcaccctgaa
     5161 cacgggtcat gctataaccg cgtacctcgg aaaactggcc ggtcatcaga ccattcgtga
     5221 cgctattctc gacgagaaaa tccgcgcggt ggtaaaaggt gcgatggaag aaagtggtgc
     5281 ggtactgatc aagcgctacg gctttgacgc agacaaacat gcggcgtaca tccagaaaat
     5341 cctcggtcgt tttgagaacc cgtatctgaa agatgatgta gagcgcgtag gccgtcagcc
     5401 gctgcgtaaa ctgagtgctg gcgaccgtct gatcaagcca ctgctcggta cgctggaata
     5461 cagccttccg cacaagaatc tgattcaggg gattgctggt gcaatgcact tccgcagtga
     5521 agatgatccg caggctcagg aactggcagc actgatcgct gacaaaggtc cgcaggcggc
     5581 gctggcacag atttccggtc ttgatgccaa cagtgaggtt gtatccgagg cggtaaccgc
     5641 ttataaagca atgcaataat ggtggaccag gcgcaggaca ccctgcgccc gaataacaga
     5701 ttgtcagata tgcaggcaac aatggaacaa acccaggcct ttgaaaaccg tgtgcttgag
     5761 cgtctgaatg ctggcaaaac cgtgcgaagc tttctgatca ccgccgtcga gcttttgacc
     5821 gaggcggtaa atcttctggt gcttcaggta ttccgcaaag acgattacgc ggtgaagtat
     5881 gctgtagaac cgttactcga cggcgacggt ccgctgggcg atctttctgt gcgtttaaaa
     5941 ctcatttacg ggttgggcgt cattaaccgc caggaatacg aagatgcgga actgctgatg
     6001 gcattgcgtg aagagctaaa tcacgacggc aacgagtacg cctttaccga cgacgaaatc
     6061 cttggaccct ttggtgaact gcactgcgtg gcggcgttac caccgccgcc acagtttgaa
     6121 ccagcagact ccagtttgta tgcaatgcaa attcagcgct atcaacaggc tgtgcgatca
     6181 acaatggtcc tttcactgac tgagctgatt tccaaaatca gcttaaaaaa agcctttcaa
     6241 aagtaagcaa cgtctgctta ctgcccctct acctgcttcg gccgataaag ccgacgataa
     6301 tactccagac gttgtagata taacggcacg ctttcatcgg gtattccgga cggaatcgcg
     6361 ttacggggag gaagtttttt cagatactcc cggaacgcct ggcttgatgc catgaaatct
     6421 acggctttat cgataagaag cggaacgttt tcacctaatt tgcccatgat gatatctccg
     6481 tattaccccg cgccgggaat gcgcggccac caattttagt ttaggttccc ggtaaagatg
     6541 acattaagga aaacgtgctg aatcctcaat aatcatccgc tgctgtttat tatctttttt
     6601 ctatagattt cttaattaat caacgaattg cgtagttttt gagcatgtta atgattacgt
     6661 tgcagaacta ttacaatgtc atcagttgtg aacaaagcac ctggtcgcgc atactagggg
     6721 ctataaattt atctttatca gaagccatca catgaaagaa gtcgaaaaaa acgaaatcaa
     6781 acgtctcagc gatcgcctgg acgccatccg ccaccagcag gccgatctgt cgctggttga
     6841 agccgcagac aaatatgccg agctggaaaa agagaaagcc acgctggaag cagaaattgc
     6901 tcgcctgcgt gaggttcata gccaaaaact gagtaaagaa gcacagaagc tgatgaagat
     6961 gccgtttcag cgcgcaatta ccaaaaaaga gcaggctgat atgggcaagc tgaagaaaag
     7021 tgttcgcgga ctggtggttg tgcacccaat gaccgcgctg ggccgtgaaa tgggcctgca
     7081 ggagatgact ggattctcta agactgcgtt ttaagcgcct cagacgactg gttcgatcat
     7141 atcaacccgc atctggcttg ccttagctgg tcaatgcggg gactctcctg tctactcgcc
     7201 taattaccaa acaattcact ccctttacgt cgatttaccg ctggttgata ttctgaaaat
     7261 gcatttttac cgaaatagcc acct
//
LOCUS       DAZPEB010000187         7218 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZPEB010000187 DAZPEB010000000
VERSION     DAZPEB010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7218
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.191"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            598..882
                     /locus_tag="ACHURJ_003510"
     CDS             598..882
                     /locus_tag="ACHURJ_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFR1282313.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            870..1355
                     /locus_tag="ACHURJ_003511"
     CDS             870..1355
                     /locus_tag="ACHURJ_003511"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1282314.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSGEAVLDDWLKQKGLKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
     gene            complement(1363..1518)
                     /locus_tag="ACHURJ_003512"
                     /pseudo
     CDS             complement(1363..1518)
                     /locus_tag="ACHURJ_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858170.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1470..2567
                     /gene="ltrA"
                     /locus_tag="ACHURJ_003513"
                     /pseudo
     CDS             1470..2567
                     /gene="ltrA"
                     /locus_tag="ACHURJ_003513"
                     /inference="COORDINATES: protein motif:HMM:TIGR04416.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            2650..2804
                     /locus_tag="ACHURJ_003514"
                     /pseudo
     CDS             2650..2804
                     /locus_tag="ACHURJ_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2855..3340)
                     /locus_tag="ACHURJ_003515"
     CDS             complement(2855..3340)
                     /locus_tag="ACHURJ_003515"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFR1282315.1"
                     /translation="MSEQKITGIDLAKTNFYLFSINAHGKPAGKTKLSRNQLLNWLVQ
                     QPKMTVAMEAGGASHYWAREIRKLDHDVILLPAQHVKAYQRCQKNDYNDAQAIAEACQ
                     HGTIRPVPIKTLEQQDVQTFLNMRRLVSMERTQLINHIRGLLAEYGIVFSKGAAELRQ
                     K"
     gene            complement(3596..4576)
                     /locus_tag="ACHURJ_003516"
     CDS             complement(3596..4576)
                     /locus_tag="ACHURJ_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFR1282316.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(4576..5775)
                     /locus_tag="ACHURJ_003517"
     CDS             complement(4576..5775)
                     /locus_tag="ACHURJ_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR1282317.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5949..6083)
                     /locus_tag="ACHURJ_003518"
                     /pseudo
     CDS             complement(5949..6083)
                     /locus_tag="ACHURJ_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6097..6435)
                     /locus_tag="ACHURJ_003519"
                     /pseudo
     CDS             complement(6097..6435)
                     /locus_tag="ACHURJ_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(6502..>7218)
                     /gene="istB"
                     /locus_tag="ACHURJ_003520"
     CDS             complement(6502..>7218)
                     /gene="istB"
                     /locus_tag="ACHURJ_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HFR1282318.1"
                     /translation="ISAAPALSQQAVDQEWSYMDFLEHLLHEEKLARHQRKQAMYTRM
                     AAFPAVKTFEEYDFTFATGAPQKQLQSLRSLSFIERNENIVLLGPSGVGKTHLAIAMG
                     YEAVRAGIQVRFTTAADLLLQLSTAQRQGRYKTTLQRGVMAPRLLIIDEIGYLPFSQK
                     EAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDAALTSAMLGRILHHSHVVQIKG
                     ESYRLRQKRKAGVIAEANPE"
BASE COUNT         1921 a         1647 c         1726 g         1924 t
ORIGIN      
        1 cggcatccgg catataggca gcatgaacgc aacacaggct ctcgtacgga acgtgggaac
       61 ctgtcgtctc gatgctaagg gagcggcgcg gagcagaggc actgcaagcg cctgagtacc
      121 gatgcgagac acaggggcag atggcttcgt agtagtgagg aagtgcctgt aatgggcatg
      181 gagcgaaggg ggtcagtcga ggcgtttgag gttaccaatc gaagctatac ccggtaatgg
      241 cgcaggttga cctgcacttg gccaagtgga ttgcccgcaa acacaagcga gcgcgaggta
      301 gtctggtgcg tgcattcgag tggttactgc gaattcgtca cgactgcccg acgctgtttg
      361 cacactggtg cttagcttac gatacttgaa tggttcaaca acaagagccg tatgacgcga
      421 gagtgtcacg tacggttccg tgggagcgta ggggggcggt gcccctgcgc cacccgatat
      481 cccctttgag tgcggttacg tccacagagg taaatgccgc tgacgcggca tctgtaatct
      541 gttactttgt gtacatcatt tgctatatat tgtcaatatg ctattgttgt aaagcaaatg
      601 atatacggag gctttatgaa atcaggtgtt cagctcaatc tcagagcaag agaatcacag
      661 cggatactca ttgatgctgc tgcagaaatt cttcataagt cgcgtacaga tttcattctg
      721 gaaatggcct gtaaagctgc tgaagatgtt attctcgacc ggcgggtgtt taattttaat
      781 gacaggcaat atgaagagtt cattgaaatg cttgatgctc cggttgcaga tgatcctgcc
      841 atcgaaaaat tgctggcaag gaaacctcaa tgggatgtgt aactgcacca gaacctttat
      901 cctcatttca tcaggtcgct gaatttgtca gcggtgaagc tgtacttgat gactggttaa
      961 agcagaaggg gctaaaaaat caggcaatcg gagccacccg gacttttgtt gtatgcagaa
     1021 aaggtacgca acaaatagtc ggtttttact cactggccac tggtagtgtc aatcatacag
     1081 aagctacagg taaccttcgg cgcaatatgc cagaccccat tcctgtaatt atacttgcac
     1141 gcctggctgt agatgtgtca tttcgaggaa aagggcttgg cgcggattta cttcatgatg
     1201 cagttcgtcg ttgttacagg gttgccgaaa atatcggtgt gcgtgcaatt atggttcatg
     1261 cgcttacgga aaacgcaaaa cagttttata ttcatcatgg ttttaagcca tcaaaaactc
     1321 aggtgcaaac attgtttctt aaactccctc aatagggagt aatgcactgg tctgggcatc
     1381 atatctggat gtgcgtcgca acaagggagc cccccggatg cgacgggcag actttgaaaa
     1441 tgttcgacca gcaacgtgat ggcaatctat acaagatctg gaaccggcta tgttcgggaa
     1501 catggtttcc gccaccagtg ctggaaaaac ggatcccgaa gtcgaatggc aaggaacgca
     1561 ttctggggat cccaacagta tccgatcgaa ttgctcaggg agcgataaaa cttttcatgg
     1621 aagaaaagct tgacccgatt ttccatgctg attcatatgg ttaccgacca ggcaaatcag
     1681 ctcatgacgc tctgaaacaa tgtgccatcc ggtgctggcg ttatagctgg atactggaag
     1741 tggacatcag tgcgtttttc gaccatgtga aacatgatct ggtgctcaaa gctctggaac
     1801 accacgggat gcctaaatgg gtcatcctgt actgtcgacg ctggatggaa gcgccaatgc
     1861 agagttgtga aaatggagaa ttaataaccc gaacacgagg cactccgcag ggcggggtca
     1921 ttagtccgct acttgctaac ttgttccttc actatgcatt tgatttgtgg atggaaagag
     1981 aatatcgggg ggtaccgttt gagaggtacg ctgacgatat tgtagtacat tgttcacgaa
     2041 tgagtgatgc aacaaggcta aagaacaggt tatcggagcg cttctcggaa gtcgggctgg
     2101 tcctgaatgc agggaaaacg aatatcgcct acattgacac gtttaaaagg agaaacgtcg
     2161 caacgagttt cacctttctc ggatatgact tcaaagtgcg tacgctgaag aatttcaaag
     2221 gcgaacggta ccgaaaatgc atgccgggtg cgtcaaatgc agcaatgcgc aaaataacag
     2281 aaacaatcaa gaagtggcgt atacatcgct caacagctga gagtttgctg gattttgcga
     2341 ggcgctacaa tacgatagtg agaggctgga tcgagtacta cggaaagttc tggtccagaa
     2401 atttcaacta tcgactgtgg agtgcaatgc agtcacgtct gctcaagtgg atgcagtcta
     2461 aatacagact ttcgaaccgg aaggctcagc gaaagctgac gctggtaagg aaggagtatc
     2521 cgaagctatt tgttcactgg tatttactac gtgcatcaaa tgagtgatca agagccgtat
     2581 gacgcgagag tgtcacgtac ggttccgtga gcagccgggg agtgaaattc tctccggctg
     2641 actcgactcg cgggttccga cagcgatgac gaacatgcgg cggtgttgta ctcgctgatc
     2701 ggcacatgcc gtctgaacaa tgtggaacca gaaaatggct gcgttacgtc attgagcata
     2761 tccaggactg gccggcaaac cgggtacgtg atctgttgcc ctagaaagtt gatctgagct
     2821 ctcagtaaat atcaatacgg ttctgacgag ccgcttactt ttggcgtaat tctgctgcgc
     2881 ctttactgaa cacaatgccg tattcagcaa gaagtccacg aatgtgattt atcagttgtg
     2941 tcctttccat tgaaaccaac cgacgcatat tcaaaaaagt ttggacgtcc tgttgctcca
     3001 gagtttttat tggtacgggt cgtatagttc catgttggca ggcctcagct attgcctgtg
     3061 cgtcgttata atcatttttc tggcaccgct gataagcttt aacatgttgt gccggaagca
     3121 atattacatc atggtcgagt ttcctgattt cccgggccca gtaatgagat gccccgccag
     3181 cctccatcgc aacagtcatt ttaggctgtt gcaccagcca gttaagcagt tgattgcgag
     3241 aaagtttggt ctttcctgcg ggtttgccat gagcattgat actgaaaaga taaaagttcg
     3301 ttttagccag atcaatgccg gtgattttct gttcagacat ggtatcccgc ttacttttgt
     3361 ggttaaagtt tatccatcat ggcgcaattg aaggctgccg tgaaagaggg ggaagactat
     3421 ctcatcggtt actgttaaac ggctaagaga aactatcttt ttcaagcaaa gaagatacgg
     3481 tgaaacaggc gatttcaccg tgaaatactg ttcaaaaaaa acgccaataa aagcaaaatt
     3541 tcttttaaaa cagccaatta aaaactggtg agttgctggc gaaaaacttg aattttcaca
     3601 gcgaattcag attatctttt agtacaagag caatcatacg atctaactta tcctgaactt
     3661 ccaacggtaa tctgccgaat tcatatgaga acgtacgtcc ctttactcgt tttcgagcaa
     3721 atttatcttt actatcaaat ttccataaca aagttacaac cgctttatcc tttactcctg
     3781 aatcaagcaa tgaagacatt tccttggtta taagccgcat aatactgttt ttaacctcgt
     3841 cttcagaaag attgtcatca gcagtaataa gctcaatact cggcgagata ttcttcacaa
     3901 cttcatcaat agatatatca cccagcctca gtcgttcact taattcagcc agttgcctgt
     3961 aatcggcgta tgtcagctca gactgaacag gaaaaagaga cacaaaatct ttaggtacgg
     4021 acgctgcttg gagggcacga gtcaccttag ctgccgacaa tccctctctt tccgcaattt
     4081 gcttctggtt cataccggcc tctttgagac gtactaaacg caacccaatt tcacgaatat
     4141 tatgttcaag tgacgtctgc aaatctttcg ccagatgctg agcttcagaa acagtaagct
     4201 catcatccgt tacaagtaca cgtaatccaa ccttgcatag caaagctgct gcccgacgac
     4261 gggaaccatc caaaatctca ataagatcgc cagtccgaat cccaatacat ggatagaatt
     4321 gttgtaaacg tatagttctg gtgatgtctt ttagtgagtc cggcgtcagg gctgtctgtt
     4381 cgcggccatt cacctcttca accacaaaag tttgagtgtc cactttgtca tgatcaacct
     4441 tgatctcagt aaattttgcc tttcggccag tattgagggt aaatacatga actggagccg
     4501 atatttcttc agtattattc aggatatttg tattcagcgt acggccaata gtcggtcggt
     4561 gctttctgtt ttccatcagt agttggccct tataaattca atacgatcaa aaactgcttt
     4621 agcgaaatct tctgctgcca tacgtgcatt tttcaaagct tcaccactgc caacataagt
     4681 aacaggattc gcagatatca cggtatcaaa cgactcgccg cttctctcaa aaccatcaag
     4741 acgtggcatt gaaacatcaa gcatgtcacc accgaaaatc tcttttgtca ggctatggca
     4801 gtatttatga tctgacttat tcgccagttt cgacatgaag ccaatatttg cctgcaaacg
     4861 gcatgaacat ccatcctgtt caataatctg tacaagttca ggtaaacgag ccaaatattt
     4921 aagcgtggaa tggaaatcaa cttgtgctgg cggcaccggt gtaaacatga tatctgcggc
     4981 agcaatggca tttttcaaaa atgcgtccag atgcgggcct gtatcaataa ggatgaagtc
     5041 gaaatcatgc ttcagcttat caataatatt ctcacgcaat atcgcgtgtt tgttttgtcc
     5101 taatagatgc tcctcgcaca gagtatccca gtttgatgca ataaaggcgt cgtcaataga
     5161 agccggcata acataaacgc cagggataac tgacggaacg ataaaatctt ccagtaattc
     5221 ctcacgactc acgttctgca acatagcctg cggtgcggtt gtatcaacca gccccaccgc
     5281 atgcaagtag ttcagaaaca tagtggctga tgactgggga tctaaatcaa ggagcagaat
     5341 acgtaaatct tctgacagca ggtgaggatg agcacgcagg gcgtgagcta cggaaacact
     5401 actcacggtt ttagacacac cgccttttag actaccgatg aatatggaat atgcctcact
     5461 ataacgatcg cgatatttag gaatgccacg atgtgcatag atatcgataa tgttctggat
     5521 ggttaatgca tattttgtcg cggttcctgc tgggcgtttt tcaaactgat agccctgagc
     5581 ctccatctca ccgactgcac catccacgct tgcgcgcgtt aattttggaa gcttagctac
     5641 agcagacttt gaatagactt gataaaaaac atcagcctga aactcctgct tttgctcctg
     5701 aatctgtttt gttagggcga gcaacatttt atctgcacgc tgcgcaacct tacttaactg
     5761 ctcaaaacta gtcattcccc ctccgtgcta tgtaggattt taacttatta caaaaaaaac
     5821 tgcattagca gggtaatgca tgcaagtgca tattgtacat gcttatatta aaaatgcgcg
     5881 cattgctgat ttattaatca tcggaatgaa tattactgta agatagtatg cctttccggg
     5941 agaaagacgc tgatttcccc cgaataatcc agttcaccac cacactccgg acaacagctt
     6001 tcttcaggct ccagatggtg tatttcgcgg gaaacatgtt ccggtaacgg acggcgatga
     6061 cgtgactgtc gcaacaggcg ggggtagtgc ttccaatcag tagaacatgt gcttttcaat
     6121 gaaggagccg atgacttttt cgtggatttc cacggaacgc gagaagcaga ttgttttacg
     6181 tgccagccgc ttgatgcggg tgcggagggt ccggttgtta cgttcaatac gttgcgtgaa
     6241 gattttgccg gtcaggtgtt tctccttcgg cacttctctg gcgtagctgc cccagtcatc
     6301 gctggtcacc atgccgatac agaacggagt caacagggct aacagttccc gacaggtctc
     6361 atcattccgt ggcccgaaag tgtaagccaa cacaccgcca gttttagtgt tgtacgcata
     6421 ccagagccag tgttgtgtca acgacggatg aaaagtgatc cacttatatc tccaccaacg
     6481 gcccaatatt gatccaccgt tttactcagg attagcttct gctataaccc cggcctttcg
     6541 tttctgtctg agtcgatagc tttctccttt gatttgaacg acatgtgagt ggtgtaagat
     6601 acggcccagc atcgctgagg tcagtgctgc atcaccggcg aacgtttgat cccactgccc
     6661 gaacggtaga ttggatgtca ggatcattgc gctcttttca taacgtttag cgatgacctg
     6721 gaagaacagt tttgcttctt tctgactgaa cggcagatag cctatttcat caatgatgag
     6781 caggcggggg gccattactc cacgctgaag cgtcgtttta taacggccct gacgttgtgc
     6841 cgtagataac tgaagtaaca gatctgctgc tgttgtgaag cgaacttgga tacctgcacg
     6901 gactgcttca tagcccatcg cgattgccag atgggttttc cccacacctg atggccccag
     6961 taatacgata ttttcattac gttctatgaa gctgagtgag cgtaacgact ggagttgctt
     7021 ctgcggtgct ccggtggcga atgtgaagtc atactcttcg aacgttttca ccgccgggaa
     7081 ggctgccatt cgggtataca tcgcctgttt acgttgatga cgtgccagtt tttcttcatg
     7141 aagcagatgc tccaggaagt ccatataact ccattcctgg tctactgcct gttgtgacag
     7201 cgcaggcgct gcgcttat
//
LOCUS       DAZPEB010000188         7196 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZPEB010000188 DAZPEB010000000
VERSION     DAZPEB010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7196
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.192"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(826..1356)
                     /locus_tag="ACHURJ_003521"
                     /pseudo
     CDS             complement(826..1356)
                     /locus_tag="ACHURJ_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
     gene            complement(1699..2349)
                     /gene="zinT"
                     /locus_tag="ACHURJ_003522"
     CDS             complement(1699..2349)
                     /gene="zinT"
                     /locus_tag="ACHURJ_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFR1282319.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(2606..3241)
                     /gene="msrQ"
                     /locus_tag="ACHURJ_003523"
     CDS             complement(2606..3241)
                     /gene="msrQ"
                     /locus_tag="ACHURJ_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFR1282320.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFNRLRKQVHNKLSV"
     gene            complement(3242..4246)
                     /gene="msrP"
                     /locus_tag="ACHURJ_003524"
     CDS             complement(3242..4246)
                     /gene="msrP"
                     /locus_tag="ACHURJ_003524"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFR1282321.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(4355..4732)
                     /gene="hiuH"
                     /locus_tag="ACHURJ_003525"
                     /pseudo
     CDS             complement(4355..4732)
                     /gene="hiuH"
                     /locus_tag="ACHURJ_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
     gene            4882..5553
                     /gene="hprR"
                     /locus_tag="ACHURJ_003526"
     CDS             4882..5553
                     /gene="hprR"
                     /locus_tag="ACHURJ_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFR1282322.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            5553..6911
                     /gene="hprS"
                     /locus_tag="ACHURJ_003527"
     CDS             5553..6911
                     /gene="hprS"
                     /locus_tag="ACHURJ_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFR1282323.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHQALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDIASLGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSASYHYLNKHNVFRITLPQ
                     RN"
     gene            complement(7019..>7196)
                     /locus_tag="ACHURJ_003528"
     CDS             complement(7019..>7196)
                     /locus_tag="ACHURJ_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310732.1"
                     /note="Hsp31 stabilizes early unfolding protein
                     intermediates under severe heat stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HFR1282324.1"
                     /translation="HLTWYFGEELKKMGMNIINDDITGRVHKDRKLLTGDSPFAANAL
                     GKLAAQEMLAAYAG"
BASE COUNT         2021 a         1483 c         1628 g         2064 t
ORIGIN      
        1 gctgagaaaa aagcgtgctt tcccaatctg gggcaaatca cgtctgaatg acacctgaaa
       61 acaggtggtg gactcaagaa ggcttggaat attcttcttt aatgttatgt aattcattga
      121 tttttcgtgt acgattttaa gacatttttt ccaagaaaaa tttttaactc tttgattttt
      181 cgactctgta tcatcggtct cgaaaaccgg agtaggggca actctaccgg gggggcaaat
      241 ccccctctct ccgccacttt atcaatgact tatctcccga cttcccgcct tacttttcct
      301 aaacagaaca atcgtagaat attcttgaag ggttagatcg tcactgtttt ctgttcgata
      361 ctgtgacatt cagcacttga ttcgctatgg atctgacagg aagctttcga gcgaaaatgt
      421 gcagttattc agtcgttttc ttatcgctca ccattattct tttagacatt catcctatga
      481 aactgccgca aagttggtgg tgggaactga ggttgcgtag agaaggggtc aataattgag
      541 tcaaaaaagg aacgcggccg tcagattaaa gccgcgggac aaagtccata atttccaaga
      601 gttgttgaat ttcccagctt ttttgaaagc ctttgtatta tactaacaag taaaaaaggt
      661 ttaaatacca aaattaacct gtatttttga taagtcttac cattgtacga tttacacagt
      721 ttctgctcct ccttgttgat agtggaaagg gtgcattgtg cacttgcgta aacatttatg
      781 acgcgttgat gtccatggtt cacatttcct tgtcagggga tcgacttatt tgcggcgagg
      841 catcattcgt aacagagtat tatctttctg aatgtagtga tgaaagagtg ctgcgccagc
      901 gtgagctgcg attaaaaagt aacctatatt tgcccaggtt tcgtgaatat tttatatcag
      961 tgctttaatt tcgctgtttg gggtaacaaa gggagacaca ttgaaaccaa ggaaactcca
     1021 cgattttcca ctgtaagcca tcaaagcaat ccccagcaga ggaagtgcaa ggaaggttat
     1081 ataaagcatg atgtgcatta gcgaagccgc tttcatttgc caggcaggtg gcggtggcac
     1141 aatagaagga tcactataac ggtgttttat aatcaggcgt gaaaacatta acacccaaac
     1201 gaatataccc gcattgtaat gtgtttctcg catcagcaga taagtactac tacctttagg
     1261 aaaccagcca cggagttcca tcgcggcata ggttattgca acgaaaagca gcgttatcca
     1321 atgtaaataa atttgagttt ttgagaatcg gttcattttt gtagctcctg taatttttac
     1381 agtcaagcta tcccacgaac cttaaaaatt ccttatacag atgtggtttt aagaatttgt
     1441 tatttaaaaa attgatagtg cacattgtaa ccagccaata taagcaatgg cgtgagcaca
     1501 ggtatctttc tctgttggcc gtattgtgta tggaatccgt tattggttaa ctcaatgcgg
     1561 gttagcttat taatatacag acacgtcata gtgatgagcg tgtctgtagc tagcttgcgt
     1621 tcagtggcgt ttgtggatgg accagtggca gttatgatga cttactaaag cggcatcgag
     1681 gcgttatcat gagaatactc aatgagacat catttcctcg accacttctt cgctactcaa
     1741 ctgatatgga taatacgttg gccagttttc catttcattt aataaagatt gctgtgaatc
     1801 attacccata aaaatgtgaa aatgagatga ttttcgtggt gcaattatat gatcgctaaa
     1861 ttgtatatat ttaggggctt tgctttcagg atctttacat tcgaataagt aacgaacgcc
     1921 tttcttgcct gatttatagg tgagtatttt gtatccatcg taatcatatt tacaggatgt
     1981 tgtttcatta tttctatgga attcaacaat gccgtcctca atgccaatca tctcgatatc
     2041 tgttgcataa cctttgtgat aataatcttt aatttcagca aatgttttag ttttatctgc
     2101 atccgctttc ttctgaaaga cggggtcaag tttgccactt tgcagtaaag gataaacgga
     2161 ttgccagacc ccatcccagt cactgagcgt tcggttttgt acattggcat catcaaaaac
     2221 accattagca gctttttgtt cgacctctgt taagggtttg ccgtgtgagt gatgaccatg
     2281 cgaaaaggca ggagcgctaa caataaagac acctaacgca acagccagtt tgtgaagacg
     2341 aatcgccaaa acagtttcct ccagagtcat tgtatatgat gtgtaatgtt atattgtaac
     2401 atatggcaaa atgcaacaac tcttaggaga tgagagcata gttttctcag gtgtacttat
     2461 ctatcttatt gaaataaaat gaattatatt gataatttga ggttgatgac aaacgaaaca
     2521 taatctgatg tgatctgcgt aggccggata gggcgctcgc gccgcatccg gcagggataa
     2581 cgcgtacttt gtcggcaata tgaagttata cagataattt gttgtgaacc tgcttgcgta
     2641 accggttaaa aagggaacgc aacttcttat accgtaaggc taaaagcagt acagccagcc
     2701 cagcgtagat gagcggctgc ggtgaaataa tcttcactga ccacagataa tgtattgggg
     2761 ccaggatcgc gacaagatag acgaaattat gcaactgttg ccagtgcttg ccgagttttc
     2821 gctgcatcgc ctgggttgaa gtaaacgcta aagcaagcaa aataacccag ctgataattc
     2881 ctaacgttaa ataaggccgg gtaattaact ccttgcctaa taacgccaga ttattcacgc
     2941 ccagctccag caatgcgtaa ctggttaaat gcagtgtcgc ccaggcaaag caccataagc
     3001 ctaacaggcg gcgagtgcgt atcaataacg gctgttttgc gtagcgtgcc agaggtgtga
     3061 ttaacaaggt cgccagcaaa aatttcagtg cagtgcgacc ggtaaaatgc tgaatatctt
     3121 tcaccggatc ggcacccagt ccaccgtgat tgatcgccca gaccagccag agaaacggca
     3181 acaatccggc aagatgcagg ctaactttca gccatgtcac ctgttttgct gtcagacgca
     3241 ttcagaaatt ctcccgcaaa tccaggccac gatacagcga tgccacctgg tcggcgtaac
     3301 cattaaacag tagcgttggc tggcgctgaa catcgaggat gccgcctgaa ccaataaatc
     3361 gttcggtagc ctgtgaccag cgcgggtgat caacatgcgg attaacgttg gcgtaaaaac
     3421 cgtattcgtc aggcgctgcc agattccagg tggttggcgg acgctcgcgg gtcagcttaa
     3481 tactgactat cgatttaatc cctttaaagc catatttcca cggcacaatc agtcgtaccg
     3541 gcgcgccatt ttgtggcggt aacgccttgc cataaacacc cacggtcatc agtgtgagcg
     3601 gatgcattgc ttcgtcgaga cgcaatcctt cgacataagg atatttcagc ccgccgccga
     3661 taaagcggtc ctgctggcct ggcatttgtt ccggtgcata aattgtttcg aaagcgacat
     3721 acttcgcatt gctggtgggt tcggcaagcg ccagcaattt gtgcagcgga aaaccaatcc
     3781 acggcaccac catcgaccat gcctccacgc agcgcatacg ataaatacgc tcttccagcg
     3841 ggaagcgacg ggttaaatca tcgtgatcga gggtcaatgg ttttgccact tcgccgctga
     3901 ttttcagtgt ccatggatcg gttttcaggc taccagcatt agcggcggga tcggctttat
     3961 ccagcccgaa ttcatagaag ttgttataac cggagacttt atctactggc gtcagtggca
     4021 ggttattttg ccaggcggca ggcttgctga actccagcgg ttttccggcg ggggcgggcg
     4081 ggcgatcgtt ccctttaaac cagctaagca gatcggcatg cgcagcgtga ggcaaagaaa
     4141 gtgcagctgc actgatgccc agtgctttta acacctgccg acgcttcata aagaataccg
     4201 actcggccgt aacatctgat tcttttaaaa attgattctt tttcataagc catcacactt
     4261 ttttgtcgtt agccagcaag agccgtttac tgttaatctg tcagggagtg tcctccctaa
     4321 cagtagcaga gatctgcttt tacatattta tgcattaact gccacgatag gttgaatacc
     4381 catattggct aagtaataaa ggcacatgat aatgctcgtt cactttatta atatgaaatt
     4441 caacggggat ctcagggaag aaactttcaa gattttgttt cttgaaatag tccccggttt
     4501 taaatacgac acggtaatcg cccgtagttg cagtttgctc gggccacagt gctttaattc
     4561 gtccatcctt atctgttttg gcggtattaa gttgtaacca gccgttatcc gccttctttt
     4621 caagagtgac tgtcacgtca gcagcgggtt ttccggtttg ctggttcaaa atgtgcacgc
     4681 taagaatgtt ttgttgtgcg gcataaacca aagaaggtaa tgaaaatgct gcaaataacg
     4741 ctttaacatg tttatatcct tgtcatgtga atgagttttg aggatagcga aaaagcggcc
     4801 agggtaacct tacagcagca ttacaatttt gtaatgaacc aggctgtttc tataacatat
     4861 gatttatggc atattatttt catgaagatt ttacttattg aagataatca aaggacccag
     4921 gagtgggtaa cgcaggggct ttccgaagcg ggttatgtca ttgatgccgt ttctgatggc
     4981 agagatgggc tttatcttgc gctgaaggat gattatgcat tgataattct ggatattatg
     5041 cttccgggta tggatggctg gcagatctta caaacgttaa gaacagcaaa gcaaacccct
     5101 gttatttgcc ttactgcaag ggattctgtc gatgacagag tcagagggct ggacagtgga
     5161 gcaaatgatt atctggtaaa acctttttca ttttctgagt tgctggcaag ggttcgggca
     5221 caattaaggc aacatcacgc tttgaattca acattagaaa tcagtggctt aagaatggac
     5281 tctgtaagtc aaagtgtgag cagggacaat atcagtatta cactgacgcg caaggagttt
     5341 cagttacttt ggctactggc ctccagagct ggcgaaatta tacccagaac ggttattgcg
     5401 agtgaaattt ggggaatcaa ctttgatagt gataccaata cggtggatgt cgccattcgc
     5461 aggctccgcg caaaagttga tgatcctttt cctgaaaagc taatcgccac aatccggggg
     5521 atgggctatt cattcgtagc ggtaaaaaaa taatgaaaag attgtctata accgtccgtt
     5581 taaccttgct ttttatattg ctgctgtctg ttgctggcgc tggaattgtc tggactctct
     5641 ataatggcct ggcaagtgag ttgaaatggc gcgatgatac aacactcatt aaccggacag
     5701 cacagatcaa gcagttgtta attgatgggg taaatccaga tacgttacct gtatacttta
     5761 accggatgat ggatgttagt caggatatct tgatcattca tggtgatggc atcaataaaa
     5821 ttgttaaccg gacaaatgtc agtgatgaca tgttaaataa catacctgct agtgagacaa
     5881 tcagcgcagc tggcatttac agaagcatta ttaatgatac tgagattgat gctttacgaa
     5941 ttaatattga tgaagtttcg ccatcattaa cggttactgt ggctaaattg gcttcagcta
     6001 gacataacat gcttgaacag tataaaatta atagcattat aatttgcatt gtcgccattg
     6061 tactttgctc agtattaagt ccgctgttaa tcagaacggg attacgagag atcaaaaagt
     6121 tgagtggtgt aacggaagcg ctgaattata acgatagccg agagcctgtt gaggttagcg
     6181 cattaccgag agaactaaaa cctcttgggc aggcgttgaa taaaatgcat caagccttag
     6241 tcaaagattt tgaacgccta agtcaatttg ctgacgatct cgctcatgaa cttagaacgc
     6301 ccattaatgc attactgggt cagaatcagg ttacgctcag tcaaaccaga agtatcgctg
     6361 aatatcaaaa aacaattgcc ggtaacattg aagagttgga aaatatttcg cggttaacag
     6421 agaacatact gtttcttgcc cgggcagata aaaacaatgt tttggtgaaa ctggactcgc
     6481 tttctctcaa taaggaagtc gaaaatttgt tggattatct tgaatatctt tcagacgaga
     6541 aagagatttg ctttaaggtc gagtgcaatc agcaaatctt tgcggataaa attttactgc
     6601 aacgaatgtt atcgaatctt attgttaatg ccattagata ttcaccagaa aaatcgcgta
     6661 ttcatataac cagttttctt gataccaacg gctatcttaa tattgatatc gccagtcttg
     6721 gaacgaaaat acatgagcct gaaaaactct tccgtagatt ttggcgggga gataattcgc
     6781 gtcattccgt aggtcaggga cttggtcttt ctttagtcaa agcgattgcc gaattgcatg
     6841 ggggaagtgc ttcatatcac tatctcaata agcataatgt gttccgaatt acgttaccgc
     6901 aaagaaatta acatagggct tcagtacgcc ataaattaac cgtgaaaaaa cgcagccacg
     6961 tcgtgagtac taacgcggct gcgattaatt atgcgcttac attcaaacgt aacagggatt
     7021 aacccgcgta agctgccagc atttcctgcg ccgccagttt acccaacgca ttcgctgcga
     7081 aaggactgtc gccggtgaga agtttacggt ccttatgtac tcgcccggtg atgtcgtcat
     7141 taatgatgtt catgcccatt ttcttcagct cttcgccgaa gtaccaggtg agatga
//
LOCUS       DAZPEB010000189         7124 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZPEB010000189 DAZPEB010000000
VERSION     DAZPEB010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7124
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.193"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..649
                     /locus_tag="ACHURJ_003529"
     CDS             <1..649
                     /locus_tag="ACHURJ_003529"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFR1282325.1"
                     /translation="TYKYADMLAMSAKKDAMVPMGGLLCMKDDSFFDVYTECRTLCVV
                     QEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYRIAQVQYLVDGLEEIGVVCQQAGGH
                     AAFVDAGKLLPNIPADQFPAQALACELYKVAGIRAVEIGSFLLGRDPKTGKQLPCPAE
                     LLRLTIPRATYTQTHMDFIIEAFKHVKENAANIKGLTFTYEPKVLRHFTAKLKEV"
     gene            740..1987
                     /gene="tnaB"
                     /locus_tag="ACHURJ_003530"
     CDS             740..1987
                     /gene="tnaB"
                     /locus_tag="ACHURJ_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFR1282326.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            2119..3294
                     /gene="mdtL"
                     /locus_tag="ACHURJ_003531"
     CDS             2119..3294
                     /gene="mdtL"
                     /locus_tag="ACHURJ_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFR1282327.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            3269..4228
                     /gene="yidZ"
                     /locus_tag="ACHURJ_003532"
     CDS             3269..4228
                     /gene="yidZ"
                     /locus_tag="ACHURJ_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFR1282328.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            4385..5134
                     /locus_tag="ACHURJ_003533"
     CDS             4385..5134
                     /locus_tag="ACHURJ_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFR1282329.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            5156..5722
                     /gene="yieF"
                     /locus_tag="ACHURJ_003534"
                     /gene_synonym="chrR"
     CDS             5156..5722
                     /gene="yieF"
                     /locus_tag="ACHURJ_003534"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFR1282330.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDRQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(5776..7113)
                     /gene="adeP"
                     /locus_tag="ACHURJ_003535"
     CDS             complement(5776..7113)
                     /gene="adeP"
                     /locus_tag="ACHURJ_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFR1282331.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
BASE COUNT         1693 a         1823 c         1803 g         1805 t
ORIGIN      
        1 aacctacaaa tatgccgata tgctggcgat gtccgccaag aaagatgcga tggtgccgat
       61 gggcggcttg ctgtgcatga aagacgacag cttctttgat gtgtacaccg agtgcagaac
      121 cctttgcgtg gtgcaggaag gcttcccgac atatggcggc ctggaaggcg gcgcgatgga
      181 gcgtctggcg gtaggtctgt atgacggcat gaatctcgac tggctggctt atcgtatcgc
      241 gcaggtacag tatctggtcg atggtctgga agagattggc gttgtctgcc agcaggcggg
      301 cggtcacgcg gcattcgttg atgccggtaa actgctgccg aatatcccgg cagaccagtt
      361 cccggcacag gcgctggcgt gcgagctgta taaagtcgcc ggtatccgtg cggtagaaat
      421 tggctctttc ctgttaggcc gcgatccgaa aaccggtaaa caactgccat gcccggctga
      481 actgctgcgt ttaaccattc cgcgcgcaac atatactcaa acacatatgg acttcattat
      541 tgaagccttt aaacatgtga aagagaacgc ggcgaatatt aaaggattaa cctttaccta
      601 cgaaccaaaa gtattgcgtc acttcaccgc aaaactgaaa gaagtttaat taatactaca
      661 gagtggctat aaggatgtta gccactctct taccctacat cctcaataac aaaaatagcc
      721 ttcctctaaa ggtggcatca tgactgatca agctgaaaaa aagcactctg cattttgggg
      781 tgttatggtt atagcaggta cagtaattgg tggaggtatg tttgctttac ctgttgatct
      841 tgccggtgcc tggtttttct ggggtgcctt tatccttatc attgcctggt tttcaatgct
      901 tcattccggg ttattgttat tagaagcaaa tttaaattat cctgtcggct ccagttttaa
      961 caccatcacc aaagatttaa tcggtaacac ctggaacatt atcagcggta ttaccgttgc
     1021 cttcgttctc tatatcctca cttatgccta tatctctgct aatggtgcga tcattagtga
     1081 aacgatatca atgaatttgg gctatcacgc taatccacgt attgtcggga tctgcacagc
     1141 cattttcgtt gccagcgtat tgtggataag ttcgttagcc gccagtcgca ttacctcatt
     1201 gttcctcggg ctgaagatta tctcctttgt gatcgtgttt ggttctttct tcttccaggt
     1261 cgattactcc attctgcgcg atgccaccag caccactgcg ggaacgtctt acttcccgta
     1321 tatctttatg gctttgccgg tgtgtctggc gtcatttggt ttccacggca atattcccag
     1381 cctgattatt tgctatggaa aacgcaaaga taagttaatc aaaagcgtgg tatttggttc
     1441 gctgctggcg ctggtgattt atctcttctg gctctattgc acgatgggga atattccgcg
     1501 cgaaagcttt aaggcgataa tctcctcagg cggcaacgtt gattcgctgg tgaaatcgtt
     1561 cctcggcacc aaacagcacg gcattatcga gttttgcctg ctggtgttct ctaacttagc
     1621 tgttgccagt tcgttctttg gtgtcacgct ggggttgttc gattatctgg cggacctgtt
     1681 taagattgat aactcccacg gcgggcgttt caaaaccgtg ctgttaacct tcctgccacc
     1741 tgcgttgttg tatctgatct tcccgaacgg ctttatttac gggatcggcg gtgccgggct
     1801 gtgcgccacc atctgggcgg tcattattcc cgcagtgctt gcaatcaaag ctcgcaagaa
     1861 gtttcccaat cagatgttca cggtctgggg cggcaatctt attccggcga ttgtcattct
     1921 ctttggtata accgtgattt tgtgctggtt cggcaacgtc tttaacgtgt tacctaaatt
     1981 tggctaaatc cttcaagaag ccagccattc gctggcttct tgcctctcag gaaatcactt
     2041 atgtccaaat ggcaactcgc ctgatcctcc ttcaccacgt atgctttgcg tcaccttact
     2101 atcaggacgc tttagcccat gtcccgcttt ttgatttgta gttttgccct ggttttactt
     2161 tatcccgccg ggattgatat gtacctcgtt ggtttaccgc gcatcgccgc cgatctcaat
     2221 gccagcgaag cgcagttgca tattgcgttc tccgtatatc tggcggggat ggcagctgcg
     2281 atgttatttg ccggtaaagt ggccgatcgt tcagggagaa agccggtcgc catacccggc
     2341 gcggcgctat ttattattgc ctcggtgttc tgttcactgg ctgaaaccag cacgttattt
     2401 cttgcaggcc gatttctaca ggggttgggc gcaggctgtt gttacgtagt ggcgttcgcc
     2461 attttgcgcg acacgctgga tgatcgacgt cgggctaaag tgctgtcatt actcaacggt
     2521 attacctgca tcattccggt gttagcgcca gtgctcggac atctgattat gcttaaattc
     2581 ccgtggcaga gtctgttctg ggcgatggca atgatgggca tcgcggtact gatgttgtct
     2641 ttgtttattt taaaagaaac gcgcccagcg tcccccgctg cttcggacaa accacgagaa
     2701 aatagcgagt cgctgcttaa tcggtttttc ctcagccgtg ttgttatcac caccctcagc
     2761 gtttcggtga tcctcacttt cgtcaataca tcgccggtat tgctgatgga aatcatgggt
     2821 tttgagcgcg gagaatacgc caccattatg gcgttgaccg ctggcgtcag catgaccgtt
     2881 tcattctcca cgccatttgc gctgggaatt tttaagccac gtacgttgat gatcacctcg
     2941 caggtgttat tccttgcagc ggggatcacc cttgccgttt caccttccca tgcggtttct
     3001 ctgtttggta tcacactgat ttgcgccggt ttctcggtag gttttggcgt agcgatgagt
     3061 caggcgttag gaccattttc attacgcgcg ggcgtagcca gctcgacctt aggtattgcg
     3121 caggtttgcg gttcgtcact gtggatttgg ctggcagcgg tggttggtat cggcgcatgg
     3181 aatatgctga tcgggattct gattgcctgt agcatagtga gcctgttgct gattatgttc
     3241 gtcgcgcctg gacgccccgt tgccgctcat gaagaaatcc atcaccacgc ttgatctcaa
     3301 tctgctgctc tgtctgcaac tgctgatgca ggagcgcagc gtgaccaaag cggcgaagcg
     3361 gatgaacgtg accccttcag cggtgagtaa gtcgctggca aagttaagag cgtggtttga
     3421 cgacccgctc tttgtgaact caccgctggg gctgtcgccc acaccgctaa tggtcagcat
     3481 ggagcaaaat ctggcggaat ggatgcaaat gagcaatctg ctgctggata aaccgcacca
     3541 ccagacaccg cgcggcctga agtttgagtt ggcggcggaa tcgccgctga tgatgatcat
     3601 gcttaatgcg ctgtcgaaac ggatctacca acgttacccg caggcgacca tcaaactacg
     3661 taactgggat tacgattcct tagatgccat tactcgtggt gaagtggata ttggtttttc
     3721 cggtcgcgaa agccatcctc gctcgcggga gctgttaagc tcgctaccgt tagccattga
     3781 ttatgaagtg ctgtttagtg atgtaccctg cgtctggtta cgcaaagatc atccggcact
     3841 gcatgaaacg tggaatctgg acaccttttt acgttatccg catatcagca tttgctggga
     3901 acagagcgac acctgggcgc tggacaatgt gttacaggag ctggggcgcg aacgcacgat
     3961 tgctatgagc ctgccggaat ttgagcagtc actgtttatg gcagcgcaac ccgacaatct
     4021 gctactggcg accgcgccgc gctactgtca gtactacaat caactccatc aactgccgtt
     4081 ggttgctctt cctctcccgt ttgacgaaag ccagcaaaaa aagctggaag ttccttttac
     4141 cctgctgtgg cataaacgga acagtcataa tccgaagatc gtctggttac gggaaaccat
     4201 taaaaacctt tacgcgtcga tggcataacc gaatcgtatg aaacgcgacc caatttcaca
     4261 ataaaatgta aaaaagttgt aataagcttg tctgaatcga acttttagcc gctttagtct
     4321 gtccatcatt ccagtaaatg attactctta tattcataat ggaccattaa gcatggagcg
     4381 aaaaatggcg actcactttg cccgagggat tttaacggaa ggacatctga tttctgttcg
     4441 tctcccctcc cagtgtcatc aagaagcccg aaacattccc cctcatcgtc aaagccgttt
     4501 tctggcgtcc agaggtttac tcgcagaact gatgttcatg ctgtatggca ttggcgaatt
     4561 gccggaaatc gtcaccctgc cgaaaggtaa accggttttc agtgataaaa atttgccttc
     4621 gttttctatt tcctatgccg ggaatatggt tggcgtggcg ttaacaaccg aaggtgaatg
     4681 tggcctcgat atggaactac agcgtgcgac gcgcgggttt catagcccac acgcgcccga
     4741 taaccacacc ttttccagca atgaatcgct atggatcagt aaacaaaacg atcctaacga
     4801 agcgcgggcg cagctcatca cgctgcgccg aagcgtgcta aaactaaccg gtgatgtttt
     4861 gaatgacgat ccgcgcgatc tgcagctgct gcccattgcc ggacgcctga aatgtgctca
     4921 tgtaaatcat gtagaagcgt tatgcgacgc ggaggacgtg ctggtgtggt ccgtggcggt
     4981 cacgcccgcg attgaaaagc tcagtgtctg ggagttagat ggcaaacaca gctggaaaag
     5041 cctgccggat attcacagcc gcgccaacaa tcctaccagc cggatgatgc gttttgccca
     5101 gctctctacc gtgaagtctt tttcgccaaa ttgatagaca accacaggag tcattatgtc
     5161 tgaaaaattg caggtggtta cgttactggg gagcctgcgc aaaggctcat ttaatggcat
     5221 ggttgcacgt accctgccga aaattgctcc ggcgagcatg gaagtcaatg cgttaccttc
     5281 cattgccgac attcccctgt atgacgctga cgtacagcag gaagaaggtt ttccagcaac
     5341 ggttgaagct ctggcggaac agatccgtca ggctgacggt gtggtgatcg tcacgccgga
     5401 atataactac tcggtaccgg gtgggctgaa aaatgccatc gactggcttt cccgcctgcc
     5461 ggatcaaccg ctggccggta aaccggtgtt gattcagacc agctcaatgg gcgtgattgg
     5521 cggcgcgcgc tgtcagtatc acctgcgcca gattctggtt ttcctcgatg caatggtgat
     5581 gaacaagccg gaatttatgg gcggcgtgat tcagaacaaa gttgatcggc aaaccggaga
     5641 agtgattgat cagggtacgc tggaccacct gaccgggcaa ttgaccgcat ttggtgagtt
     5701 tattcagcga gttaagatct aaataaaaaa cccgccagta atcatgcatg gcgggttttt
     5761 aatgcgctat cgattttaat gagcgtcgat aaatacaatc ttcaggataa acagcagcgc
     5821 aacgatgatt acgcacgggc taaggtcacg cagacgcccg gtaccaatct tcatcacgca
     5881 gtaggagata aagcccagcg caataccttc ggtaatcgaa aagctgaacg gcatcatcac
     5941 ggcggtaata aacgccggaa cagattcagt aagatcctgc cagttcacgc gagcaagact
     6001 tgaggtcatc aacacgccaa cgtagatcaa cgcgccagct gcagcgtagc ctggcaccat
     6061 tcccgccagc ggcgacagaa agataaccag caggaacagc agaccaacaa ccactgccgt
     6121 cagaccggta cgaccgccaa ccgatacgcc ggaagaggac tcaatataag ccgtaacgga
     6181 agaagtaccg ataaacgaac cggtcacgga agagatactg tcgacataca gcgcttgctt
     6241 catgcgcggg aatttcccct tctcatccgc cagacctgct ttatcggtca cgccaatcag
     6301 cgtaccggag gagtcaaaca agttgaccaa catgaaagag aaaatcaccc ctgccagccc
     6361 gaggttaaac gacccggcta aatctacatg acccacaact gtcattacgc tcggcggcgc
     6421 agaaacgatg ccattgtagt gcacatcacc cagcatccag cccagcagcg tcgtcaccac
     6481 gatagaaacc agcaccgctg cgtgaatgtt gcgcgaggcc agaatagcaa tgatgaagaa
     6541 gccgaggata cccagaagta cgctgtgaga agtcagatta ccgatgctca ccagcgtttc
     6601 cgagttagcg acaatcacac ctgcgttttt cagccccatc atgccgataa acaggccgat
     6661 accgctggta atacccacac gcagacttac cggaatgtta gcaatcatcc agtaacgaac
     6721 gcggaaaatg gtcaaaagaa gcagtccgac agcgccccag aagattgcgc ccataccgat
     6781 ctgccacggc agccccattg cctggacaac cacaaaagcg aaaaaggcgt taagccccat
     6841 cgcaggagcc agcgccaccg gaaggtttgc aaacaatccc atcataatgc taccgaaagc
     6901 agcaatcaga caggttgtta cgaagacggc actggtgtcc atgccagcaa cgcccagaat
     6961 ttgtgggtta acaaaaacga tgtaaaccat cgtcaggaag gtggtaaaac cggcgatcac
     7021 ttcggtccgt gccgtcgtgc catgttcgcg cagtttaaac acgcgttcca gcatcccctg
     7081 gccagaagcc tgggttgtgt gttgttgact cattatctat ttcc
//
LOCUS       DAZPEB010000190         7056 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZPEB010000190 DAZPEB010000000
VERSION     DAZPEB010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7056
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.195"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            1..2904
                     /gene="yfaL"
                     /locus_tag="ACHURJ_003536"
                     /pseudo
     CDS             1..2904
                     /gene="yfaL"
                     /locus_tag="ACHURJ_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
     gene            complement(3032..3754)
                     /gene="ubiG"
                     /locus_tag="ACHURJ_003537"
     CDS             complement(3032..3754)
                     /gene="ubiG"
                     /locus_tag="ACHURJ_003537"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFR1282332.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     LTNTFKLGPGVDVNYMLHTQNK"
     gene            3901..6528
                     /gene="gyrA"
                     /locus_tag="ACHURJ_003538"
     CDS             3901..6528
                     /gene="gyrA"
                     /locus_tag="ACHURJ_003538"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFR1282333.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            6677..>7056
                     /locus_tag="ACHURJ_003539"
     CDS             6677..>7056
                     /locus_tag="ACHURJ_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 family protein"
                     /protein_id="HFR1282334.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIVLLSAGVWALQYAGSGPEKTLSP
                     LVMHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLD"
BASE COUNT         1693 a         1743 c         2013 g         1607 t
ORIGIN      
        1 ggctatggag atggtccttc cactgcggcg ggtggcttta tgtatctcgg cttaagtgaa
       61 gttacctttg atattgccga cggaaaaacg ctggttatcg gcaatacaga gaatgacgga
      121 gctgttgact ctattgctgg taccgggttg atcaccaaaa caggttccgg cgatctggta
      181 cttaatgcag ataacaatga ctttactggc gagatgcaga ttgaaaacgg tgaagttacc
      241 ctgggccgca gcaactccct gatgaatgtc ggcgatacgc attgccagga cgatccgcaa
      301 gactgctacg gtctgacgat agggagtatt gataagtacc agaatcaggc agagctaaac
      361 gttggctcca cccaacaaac ctttgtgcac tcattgacgg gctttcagaa tggcacttta
      421 aatatcgatg ctggtggcaa tgttactgtt aatcaaggta gttttgctgg caccatcgaa
      481 ggtgctggtc agctcaccat tgcgcaaaac ggcagctacg tgctgtcggg ggcgcagtcg
      541 atggcgctaa ccggcgatat agtcgttgat gatggtgcgg tgctttcgct ggaaggcgac
      601 gcggcagatc tcgccgctct ccaggacgat ccgcagtcga tcgtgttaaa cggcggtgtg
      661 ctcgatctct ctgatttctc cacctggcag agcggcacat catacaatga tggccttgaa
      721 gtcagtggca gcagcggaac ggttatcggc agtcaggatg tggtagatct tgcaggtggc
      781 gacaatttgc atatccgcgg cgacgggaaa gatggcgtct acgtggtggt cgatgcgagc
      841 gacgggcagg taagtctggc aaacaataat agttatttgg gcacaacaca aatcgcctcc
      901 ggtacgctga tggtgagcga caactcgcag cttggagata cccactataa ccgccaggtt
      961 atctttaccg ataagcaaca agaaagcgtg atggagatta ctgccaatgt cgatactcgc
     1021 tctacgacga ctgagcatgg gcgtgatatt gaaatgcgcg ccgacggtga agtggcagtt
     1081 gatgcggggg tagacacgca gtggggcgca ctgatggctg acagcagcgg gcagcatcag
     1141 gatgagggta gcacatttac taaaacgggg gcgggtacgc tggagctgac cgccagcggt
     1201 acaacgcagt cggcagtacg tgtcgaagaa ggcaccctga aaggcgatgt tgcggatatc
     1261 tttccttatg cttcgtcact gtgggtcggt gatggggcga cgttcgttac tggcgcggat
     1321 caggatattc agtctatcga tgctacttcc agcggcacta tcgatatcag cgatggtacg
     1381 gttttgcgtc tgaccgggca ggatacttcc gtcgccctta atgcctcatt gtttaacggc
     1441 gatgggacgc tggtgaatgc taccgatggc gtgacgttga caggtgagct taataccaac
     1501 cttgaaactg acagcctgac ctatcttgcc gacgtgacgg ttaatggtga tctgaccaat
     1561 acgtccggtg cggttagcct gcaaaatggc gtcgctggcg atacgctaac ggtaaacggt
     1621 gattatactg gcggcggtac gctactgttc gacagcgaat taaacggcga tgactcggta
     1681 agcgatcaat tggtgatgaa cggtaatact gctggcaaca caactgtggt ggttaactcc
     1741 attacaggga ttggtgagcc gacatcgaca ggcattaaag tggttgattt cgcagctgat
     1801 cccacgcagt ttcaaaacaa tgcacagttc agtctggctg gcagtggcta cgtcaacatg
     1861 ggagcgtatg actacacgct agtggaagat aacaacgact ggtatctgcg atcgcaagaa
     1921 gtaacgccgc catcgccacc tgatccagac ccggacccaa cacccgatcc tgacccaaca
     1981 cccgatccaa tccccgctta ccagccggtg ctgaatgcca aagttggcgg ttatctcaat
     2041 aacctgcggg cggcaaatca ggcgtttatg atggagcgac gcgatcacgc tggtggcgat
     2101 ggtcagacgc tgaatttacg tgttatcggc ggacgttatc attacacggc ggcagggcaa
     2161 ctggctcagc aggaagacac ttctacggtg cagcttagcg gcggcttgtt tagcgggcgc
     2221 tggggcacgg atggcgagtg gatgcttggg gcggttggtg gctacagtga taatcagggc
     2281 gacagccgct cgaatatgac cggaactcgc gccgataacc agaaccacgg ttatgccgtt
     2341 gggctgacct caagctggta tcagcacggt aattagaagc agggggcctg gctggatagc
     2401 tggctgcaat acgcgtggtt taacaatgat gtttccgaac atgaagatgg cgtggatcgt
     2461 taccattcgt cggggattat cgcctcgctg gaagccgggt atcagtggtt accggggcgt
     2521 ggtgtggtga ttgaaccgca ggcgcaggtg atttatcagg acgtgcagca ggatgatttt
     2581 accgccgcta accatgcgcg cgtgtcacaa tcgcaggggg atgatattca gacgcggctg
     2641 ggtttacaca gcgaatggcg taccgcggtt ggtgtcacac caacattaga tctgaattat
     2701 tatcacgatc cccatgcgac ggaaattgaa gaagatggca gcactatcag tgacgatgcg
     2761 gtgaagcgac ggggtgaaat aaaagtggga gtcacgggca atatcagtca gcgagtgtcc
     2821 ctgcgcggca gcgtggcgtg gcagaaaggg agtgatgatt ttgcccagac ggcagggttt
     2881 ttgtcgatga cggtgaaatg gtaatcactg tctagtcggc acggttgccg gatgcggcgt
     2941 aaacgcctta tccggcctac ggggagcatt tgtaggcctg ataagacgcg gcaagcgtcg
     3001 catcaggcat tggctcggcc aaacaacaac ctcacttatt ctgcgtgtgc agcatatagt
     3061 tcacatccac gccggggccg agtttaaaag tattagtgag cgggttgtaa tgtagcccag
     3121 ttatatgtcg ctctttaagg cttgtctgat ctacccagcc aagcagttct gccggtttaa
     3181 taaacttctt cacatcatgc gtgcctttgg gcaccatgcg caaaatatat tccgcgccaa
     3241 ccaccgccat cagccatgac ttgccgttgc ggttaagcgt cgagaaaaag acatcgccgc
     3301 ctggtttcac cagttgcgca caggctctga ccactgactg cggatcgggg acgtgctcca
     3361 gcatctccat gcaggtcacc acatcatact gcccggcatg ttttgccgcg tgctcttcca
     3421 cggtttcctg cacataatcc acctgaatgc cgctttccag cgcgtgcagt tttgccacct
     3481 gcaatggctc aaagcccata tccagaccgg tcaccgtcgc gccttcgcgc gccatactct
     3541 cggccagaat gccgccgcca caaccgacat cgagcacctt tttgccaaat aaaccgccag
     3601 cacgctcggc aatatagccc agacgcagcg ggttaatgcg gtgcaacggt ttgaactcac
     3661 cttcaagatc ccaccagcgg gaggcgacgg cttcaaattt agcgatctct tcgtggtcta
     3721 cgttatggtt ttccggcgat ttttcggcat tcattggcac ttctactccg taattggcaa
     3781 gacaaacgag tatatcaggc attggatgtg aataaagcgt ataggtttac ctcaaactgc
     3841 gcggctgtgt tataatttgc gacctttgaa tccgggatac agtagaggga tagcggttag
     3901 atgagcgacc ttgcgagaga aattacaccg gtcaacattg aggaagagct gaagagctcc
     3961 tatctggatt atgcgatgtc ggtcattgtt ggccgtgcgc tgccagatgt ccgagatggc
     4021 ctgaagccgg tacaccgtcg cgtactttac gccatgaacg tactaggcaa tgactggaac
     4081 aaagcctata aaaaatctgc ccgtgtcgtt ggtgacgtaa tcggtaaata ccatccccat
     4141 ggtgactcgg cggtttatga cacgatcgtc cgtatggcgc agccattctc gctgcgttac
     4201 atgctggtag acggtcaggg taacttcggt tccatcgacg gcgactctgc ggcggcaatg
     4261 cgttatacgg aaatccgtct ggcgaaaatt gcccatgaac tgatggccga tctcgaaaaa
     4321 gagacggtcg atttcgttga taactatgac ggcacggaaa aaattcccga cgtcatgcca
     4381 accaaaattc ctaacctgct ggtgaacggt tcttccggta tcgccgtagg tatggcaacc
     4441 aacatcccgc cgcacaacct gacggaagtc atcaacggtt gtctggcgta tatcgatgat
     4501 gaagacatca gcattgaagg gctgatggaa cacatcccgg ggccggactt cccgacggcg
     4561 gcaatcatta acggtcgtcg cggtattgaa gaagcttacc gtaccggtcg cggcaaggtg
     4621 tatatccgcg ctcgcgcaga agtggaagtt gacgccaaaa ccggtcgtga aaccattatc
     4681 gtccacgaaa ttccgtatca ggtaaacaaa gcgcgcctga tcgagaagat tgcggaactg
     4741 gtaaaagaaa aacgcgtgga aggcatcagc gcgctgcgtg acgagtctga caaagacggt
     4801 atgcgcatcg tgattgaagt gaaacgcgat gcggtcggtg aagttgtgct caacaacctc
     4861 tactcccaga cccagttgca ggtttctttc ggtatcaaca tggtggcatt gcaccatggt
     4921 cagccgaaga tcatgaacct gaaagacatc atcgcggcgt ttgttcgtca ccgccgtgaa
     4981 gtggtgaccc gtcgtactat tttcgaactg cgtaaagctc gcgatcgtgc tcatatcctt
     5041 gaagcattag ccgtggcgct ggcgaacatc gacccgatca tcgaactgat ccgtcatgcg
     5101 ccgacgcctg cagaagcgaa aactgcgctg gttgctaatc cgtggcagct gggcaacgtt
     5161 gccgcgatgc tcgaacgtgc tggcgacgat gctgcgcgtc cggaatggct ggagccagag
     5221 ttcggcgtgc gtgatggtct gtactacctg accgaacagc aagctcaggc gattctggat
     5281 ctgcgtttgc agaaactgac cggccttgag cacgaaaaac tgctcgacga atacaaagag
     5341 ctgctggatc agatcgcgga actgttgcgt attcttggta gcgccgatcg tttgatggaa
     5401 gtgatccgtg aagagctgga gctggttcgt gaacagttcg gtgacaaacg tcgtactgaa
     5461 atcaccgcca acagcgcaga catcaacctg gaagatctga tcactcagga agatgtggta
     5521 gtgacgctct ctcaccaggg ctacgttaag tatcagccgc tttctgaata cgaagcgcag
     5581 cgtcgtggcg ggaaaggtaa atctgccgca cgtattaaag aagaagactt tatcgaccga
     5641 ctgctggtgg cgaacactca cgaccatatt ctgtgcttct ccagccgtgg tcgcgtctat
     5701 tcgatgaaag tttatcagtt gccggaagcc actcgtggcg cgcgcggtcg tccgatcgtc
     5761 aacctgctgc cgctggagca ggacgaacgt atcactgcga tcctgccagt gaccgagttt
     5821 gaagaaggcg tgaaagtctt catggcgacc gctaacggta ccgtgaagaa aactgtcctc
     5881 accgagttca accgtctgcg taccgccggt aaagtggcga tcaaactggt tgacggcgat
     5941 gagctgatcg gcgttgacct gaccagcggc gaagacgaag taatgctgtt ctccgctgaa
     6001 ggtaaagtgg tgcgctttaa agagtcttct gtccgtgcga tgggctgcaa caccaccggt
     6061 gttcgcggta ttcgcttagg tgaaggcgat aaagtcgtct ctctgatcgt gcctcgtggc
     6121 gatggcgcaa tcctcaccgc aacacaaaac ggttacggta aacgtaccgc agtggcggaa
     6181 tacccaacca agtcgcgtgc gacgaaaggg gttatctcca tcaaggttac cgaacgtaac
     6241 ggtttagttg ttggcgcggt acaggtagat gactgcgacc agatcatgat gatcaccgat
     6301 gccggtacgc tggtacgtac tcgcgtttca gaaatcagca tcgtgggccg taacacccag
     6361 ggcgtgatcc tcatccgtac tgcggaagat gaaaacgtag tgggtctgca acgtgttgct
     6421 gaaccggttg acgaggaaga tctcgatacc atcgacggca gtgccgcgga aggggacgat
     6481 gaaatcgctc cggaagtgga cgttgacgac gagccagaag aagaataatt ttacttcttc
     6541 atgccaaaag ggagctatct cccttgtttg aattgaaaag tccaggctgc aaagtctggg
     6601 cttttgtcgt attagggcac ggtaaagttt ggctgtgccc gtaaaaaatg gctggctata
     6661 cacaaggaat gtggcaatga gtggtgaaaa aaaggcgaaa ggctggcggt tctatggtct
     6721 tgtaggtttt ggcgcaatag tactgctttc cgctggcgtc tgggcgttgc aatatgctgg
     6781 cagtgggcca gaaaaaacgt tgtcgccgct ggtgatgcac aacaatctgc aaatcgatct
     6841 caatgagccg gacctctttc tcgacagcga ctctctgagc cagcttccca aagatctcct
     6901 caccattccg tttctccacg atgttctgag tgaagatttc gttttctatt atcagaatca
     6961 tgccgatcgt ctgggcattg aaggcagcat tcgtcgcatt gtctatgaac acgatctcac
     7021 gctgaaagat aaactctttt cgtcactctt agatca
//
LOCUS       DAZPEB010000191         7015 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZPEB010000191 DAZPEB010000000
VERSION     DAZPEB010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7015
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.196"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..311)
                     /locus_tag="ACHURJ_003540"
     CDS             complement(<1..311)
                     /locus_tag="ACHURJ_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFR1282335.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     V"
     gene            complement(305..1543)
                     /gene="preT"
                     /locus_tag="ACHURJ_003541"
     CDS             complement(305..1543)
                     /gene="preT"
                     /locus_tag="ACHURJ_003541"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFR1282336.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAQPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIGGGDVTMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFTSARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTMVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(1940..2179)
                     /locus_tag="ACHURJ_003542"
     CDS             complement(1940..2179)
                     /locus_tag="ACHURJ_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFR1282337.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(2182..2901)
                     /gene="sanA"
                     /locus_tag="ACHURJ_003543"
     CDS             complement(2182..2901)
                     /gene="sanA"
                     /locus_tag="ACHURJ_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFR1282338.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(3051..3935)
                     /gene="cdd"
                     /locus_tag="ACHURJ_003544"
     CDS             complement(3051..3935)
                     /gene="cdd"
                     /locus_tag="ACHURJ_003544"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFR1282339.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(4065..4760)
                     /locus_tag="ACHURJ_003545"
     CDS             complement(4065..4760)
                     /locus_tag="ACHURJ_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFR1282340.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMHIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(4757..5155)
                     /locus_tag="ACHURJ_003546"
     CDS             complement(4757..5155)
                     /locus_tag="ACHURJ_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFR1282341.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            5394..6344
                     /gene="dusC"
                     /locus_tag="ACHURJ_003547"
                     /pseudo
     CDS             5394..6344
                     /gene="dusC"
                     /locus_tag="ACHURJ_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
     gene            complement(6732..6815)
                     /gene="yohP"
                     /locus_tag="ACHURJ_003548"
     CDS             complement(6732..6815)
                     /gene="yohP"
                     /locus_tag="ACHURJ_003548"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFR1282342.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
BASE COUNT         1827 a         1794 c         1732 g         1662 t
ORIGIN      
        1 aatactttgt ccgggtaatc ttctttcagc cgacgcaggg cggccagatt ctcctccagc
       61 ggatgctcgg caatctgctc catatttttg aagccgataa aaccggtatc ttctttcacc
      121 agatgatcaa aacgcggcga gacttcgttg gcgataaaaa agccgatcgt tttaaacacc
      181 acaccgcccc aacctgtgtc gtaggctttg gcacacatct catagcagtt gcctaccggc
      241 gaagaagaga ggcagaacgg gttgggaaac ttcacgccgc aaaaagtaat cgaaagatct
      301 ttcgttaaca tgagcaagct ccctctaaat agtgatgaat cgcctcggcg gcttctttcc
      361 cggttttcac ggcatagacc atggttttgt caccctcaac aatatcgcca gcagcaaaga
      421 cttgcgggtc gcgggtctgg taattttgtg ttttgatggt gttacgctgc ggctctaact
      481 ccgcaaaggc atctagtctg gcatgctgac cgacggcgag aataatttta tctgccgcca
      541 tcgtcagttc gcccgataac cgtacatgct taaacgtgac tttattgcct tccacggcta
      601 ctggcgtgaa tccatcgatg atcgaaacgc ccagctcccg ggcgctggta aactcttttt
      661 cgcttgccgg aaactcatct aactcttcac gcgctacgca agttaccgcc tgacagccga
      721 gaactttcag cgtgctggct acgtccatcg tgacatcacc gccgccgata attaatgcgc
      781 tttgtggaat gctgatatcg ccttgcgcct gtcgtgcacg ttgcaagaag tcgacggcaa
      841 tctcaacgtc actatgctca aacagcggta gcccggaacc gcttgataac cccacggtga
      901 ccagtaccgc gcggttttct gctttcagct gctcaagggt gagtgtgtta ccgacttcgt
      961 tgttgcactt aatggtcacg cccatttttt caatacgggc gatctctgca tccagtactg
     1021 attgcggtaa ccggaattgc ggaatgccgt tacgcaacca gccaccgggt tgtgcttctt
     1081 tctcatagat cgtgacgtca taaccctggt ttgtcagtgt cacactggcc tgcaatccgg
     1141 caggacctgc gccaataatc gcgactttgc cgagcgtttt agtaccgggc tgataaattt
     1201 ccatcccggt ttgttgttca aaatcagtaa caaaacgctg taagcggccg atatcaatgg
     1261 gagcatcaac accggcacgg gtacaaccgc tttggcataa tttctccgtc gggcacactc
     1321 tggcacaaac ggcaccgagg gcattatttt cgcgaattgt ctcggcagcg cctttaaaat
     1381 tacgaaagta gattgagcga ataaatttcc ccggatcggt ctgtgccggg caagcctgac
     1441 tacagggagc gtcgtgacat aataaacagc gagaggcttc tttaatcgcc agtaacggcg
     1501 taaaagccgg agtgagttca tccagataat tttgttgcgg catgtgatct tccttaatgc
     1561 tatgagtaat taaggcgaac gctaaatata ctcgtcatac ttcaagttgc atgtgctgcg
     1621 tctgcgggtt attcggccca tccatgggcc tcacccctta tggggccgct gcaggcagcg
     1681 ttcaaatccg ctctatgcgg atttgtcgct caccccagtc acttacttat gtaagctcct
     1741 ggggactcac tcacttgtcg ccttcctgca actcgaatta tttagagtat aaatattgcg
     1801 tgctgatatt aaggctatgc gcgtggggaa atttatactg tgaaaggatt cacaaatagc
     1861 tgcggttcag aatgttatat ccgcagctta ttgagtgaag taataattat tctcaataat
     1921 taacagaaaa gagaaaatat taacgataga aaataagata aacaatcatg ccgatagata
     1981 aaattccgaa gccgatatat gccaccatat aagcgaaaaa gagtccggga ttttttgctc
     2041 gccagacata caccggttcc ggtgcatttt taacccgttt atcaatcgcg cctgcgcgcc
     2101 agcctttcca tgcttcgcgg aaacaaaata tcggaataag gaaaaaaacg gcaacgacaa
     2161 aaaactgctg tacatccata actactttcc ttgtttcttt tgtaattcaa gtaactgttc
     2221 gggtgtgacg gcgggatacc cctgcgcatc ttccggcacc tggtgcatag ccggaatagg
     2281 gaccagcggc cctaaaaaac gcggttcacg tttaaaaata taaaggtcag ccagcgcacc
     2341 gaaacgggcg gcaaattcgc gaatacgtac tgacagcata tctttcggtg acggtacggc
     2401 ataacactga gcctgaatcc ccatatgcag cgcaataaat aatgcccgct cacagtggaa
     2461 acgttgggtg ataataatga aatcattagt atcgaaaact ttgcgtgtac gcacgatgga
     2521 atccagcgta cgaaagcctg cgtaatcgag aacaatatct gatgggtcga caccagcagc
     2581 gattaaatct ttgcgcatgg tcatcggctc attataactt tgcaatgcgt tatcgccgct
     2641 cagtaataga taatttacct taccgctgtt ataggcatta atcgctcctt gaatgcggta
     2701 gcgataatac tgattaatta cgccagtacg ataatatttt gctgttccga gcaccacacc
     2761 gacctggcgg taggggagat cctgcaattc gtcgtagata taaggcgcgg ttttccagct
     2821 catccagcga tcgaggccga gcacagtcaa caacagcaag ccgatcagga ctaacaggct
     2881 gaggaacacg cgctttaaca tgcaggagac tcgatctaag gaaaatttcc atcaggctac
     2941 tttacccgct ggttttgcgc aagaaacggc aaactgtttg ccgggaattt cgacaccagg
     3001 agagggctgg caagccggac aaggcgttca cgccgcatcc ggcaccaggc ttaagcgaga
     3061 agcactcggt cgatactgtg acagccgaga gctttcagcg ttgcggaggt ggcatcccac
     3121 tgaatcaacg gcgcatcggc tttttctgcc agaaccgcgc gctggatatc cgggtaatca
     3181 taacccttga gattcaacag aattaacgct ccctgcaacg gtggcagagt cgggttgaat
     3241 gcggcgtttt cagcgtagct gccactgaaa atacgaccgt ctttacattc cagcgcgaca
     3301 ccgcttggcg acttactgta aggcatgtgc gaacggtttg ccgctgcaat cgccgcctga
     3361 gaaagcgcat caccagtcag cgcatagccg tgatcctgct cgtccatcag cagcgtttta
     3421 atctccagat ctttcggccc aaaggcatcc ggcagatagt cacgcagcgc gtgtgcctcg
     3481 cggcccggca gatgaatacg cagatccaga ccgctgttca gttcattcat aaactgacgg
     3541 cagtgaccac aaggcgtgta gttaacggtg atggctgcaa gcgctttttc accactcaac
     3601 caggcgtggc tgatcgcgct ttgttcggca tgaacggttt gctgcattgt cgcaccaata
     3661 aattccatat tggcaccgaa ataccaggtt ccgctcacac cgcgcgcaat tgcgccaaca
     3721 ttaaaattcg acaatggcgt acgcgcacag gccgctgcca gcggaagtag tgcgaatgcc
     3781 agcgcgtctt cgtccagccc cgttgcgctc ttcagcgatg agacttgctc cccggtcaac
     3841 aaagcgggga agtacttgtc tgccagaata ggttccagtg cagattgcaa gttatccgca
     3901 agttgggcaa aagcggtttg aaaacgtgga tgcatggcgt tgcctcataa tcatgtaatg
     3961 ggataacagt ttacgaaccc gttatggctt tatatgtgat ctgaatctca ttaattatgc
     4021 aacgcgtgaa acttacatca aaaatgcgcg acgcatcgca aattttagcc cattactgcc
     4081 agaataatcg ggaaaaggaa cggtgcgatc agcgaagtaa ttatcccgca taacaccagc
     4141 gctagcgaac taaatgcacc ttcctgataa tccagctcgg cgcagcgcgc cgtaccgagg
     4201 gcgtgcgagg cagtccccat tgccagaccg cgcgcagctt tggtacgaat atgcatcgca
     4261 ttaagcaatg tatggccaaa taccgcgccg aggatgccga cgaaaatcac gcaaactgcg
     4321 ctgattgccg gaataccgcc aatactgccg ccaaccgcca ttgcaatagg cgtggtgact
     4381 gatttcggca ggattgacgc ggcgatttcc ggtgaagcgc ccatcaacaa tgccacggaa
     4441 gtccccgtca ccatcgcaac cacgctgccg ataaaacaga tggtgatgat cgatttccag
     4501 cgcgcgcgga tctggtgtag ctgctcatat aaaggatagg ccagcgcgac gaccgccggt
     4561 tgcagcagat cgtttagcac ttcgctacct ttaaagtagc tgtcgtaaga gatgccggtc
     4621 agcattaaaa aagggatgat caccaccatt gctaccagca acgggttaag caagggaaac
     4681 ttataccgtg ccgccagttt gcgggcggca aaaaagacga tcaatgtcag tggtaatgac
     4741 caccagatat tagccatcat tcttctgatc ctttctgacc tacgacttta cgttcaccgt
     4801 gcaccagttg cgaactccag ctcaccacca gaaaaaccac cagtgtactg acagcacagg
     4861 aaaccactac cgggccaaac tgtgcgcgga gcaaatcaaa atattgcatg acgcctacgc
     4921 caatcggcac aaacaatagc gccatatagc gaatcagtac gtagcacccc ggattgaccc
     4981 attttgccgg aagaatttgc aaggccagca ggacaaacag gatcagcatc ccgatgatgc
     5041 tgcccggaat ggttaccggt agcagggaag caatgaaaat gcctgcatac aggcaggcat
     5101 aaatcaggac gaaagcgcgt aaatattgcc agataatgtt cagtgtctta ctcataatca
     5161 acaccccaga tgagatgaat tcatcataca attaattcgt aaaacgtgct accgatcaca
     5221 ttaagaaatc agcgcttatt ttttcgctcc ccggcattac gcaatgcgca acgaactcag
     5281 gcatacatca agcatagaga gggattacag tttgcagcaa tccctctttg cagaccttta
     5341 atccgttgac cataggcgct aacatagcgc ctcatttttt tgcgggtgaa gatatgcgtg
     5401 tgttactggc tccgatggag ggtgtgcttg actctctggt gcgtgaattg ctgaccgaag
     5461 ttaacgacta cgatctgtgc atcaccgagt ttgtccgcgt ggtggatcaa ctgctgccgg
     5521 taaaagtttt tcatcgcatt tgccctgagc tacaaaacgc cagccggaca ccatccggta
     5581 cgctggtgcg cgtgcagttg ttgggtcagt tcccacaatg gctggcagag aatgccgccc
     5641 gtgcggtgga attaggttcc tggggcgtgg atctcaattg cggctgcccg tcgaaaacgg
     5701 tcaacggtag cggtggtggg gcgacgttac tcaaagatcc tgaactcatc tactagggtg
     5761 caaaagcgat gcgtgaagct gtaccggcgc atttgcccgt cagcgtgaaa gtgcgtctgg
     5821 gctgggacag cggtgagaag aaatttgaaa tcgccgatgc ggttcaacag gctggtgcta
     5881 cggagctggt ggtgcatgga cggacaaaag agcagggtta ccgcgcggag catattgact
     5941 ggcaggcgat tggcgagatt cgccagcggc tgaatattcc ggtgattgcc aacggtgaaa
     6001 tctgggactg gcagagtgcg caacaatgta tggcgatcag cggctgcgac gcagtgatga
     6061 ttggtcgcgg ggcgctcaat attcccaacc tgagccgggt ggtaaaatat aacgaaccgc
     6121 gaatgccgtg gccggaggtg gttgctttgc tgcaaaaata tacccgtctg gaaaagcagg
     6181 gcgataccgg gttatatcac gttgcgcgga ttaaacagtg gttgagttat ttgcgtaaag
     6241 aatacgatga agcaacggaa ttatttcagc atgttcgggt gttgaataat tcccctgata
     6301 ttgcaagggc tattcaggca attgatatcg ataagctgcg ataatagtgg aatcgcagtt
     6361 tatcagagac aatgaatcgc ttattcagct tcatcatctt gttgcgctcc tttcatgagc
     6421 taagccacat attgccactg gcgcaaggag cgcgcagggg gaggacaatc gcctcctccc
     6481 cctgcccccc cgggctctgg cgaacaaaat cgccgctgcg cggtaccttc agcttatccc
     6541 cttcggctac cgggtcgggc gcgaggtaac gtccttgtaa gacgcgccct cagcccacat
     6601 ccatgtgggc tgccccggcc ttcggggaac gcttcagcga ttttgacgcc accaacaccg
     6661 gagctgtttg caaatcaagt acacaaaata tctagttcct gacctgacaa cttatatgcc
     6721 atatctgatt tttaaaatat catcttaaat acgccagtca ccaccagtag cccaatcagg
     6781 aaaataatca atacagccca gagtataatt ttcattggcg ttcctttatt tgtgagtgtt
     6841 gaataagtat aggaaaaaac gaagcccttt gccgttatcc ataaacgatt aagataaatc
     6901 ggaagatatc attcacttag tctatatttt tctgggtatg tcgttttatt gctactgatg
     6961 tattgccacg cgaaactaaa aaatatatcg caacacacgg taatctccgc gcacc
//
LOCUS       DAZPEB010000192         6994 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZPEB010000192 DAZPEB010000000
VERSION     DAZPEB010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6994
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.197"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..247
                     /locus_tag="ACHURJ_003549"
     CDS             <1..247
                     /locus_tag="ACHURJ_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282343.1"
                     /translation="HARLFGFTAEDIMDFWQHKAPQKYSAFELAFEFGHRVIAELILN
                     TLNKMAESFGFTDNPRYIAEKNYMEALLKKASPHTVR"
     gene            complement(216..1940)
                     /gene="aceK"
                     /locus_tag="ACHURJ_003550"
     CDS             complement(216..1940)
                     /gene="aceK"
                     /locus_tag="ACHURJ_003550"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HFR1282344.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     LAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAALVEWLREILPGKTT
                     AELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRVFKVI
                     KDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMELLLQE
                     AAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAAANIF
                     PGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSVSPGD
                     VFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRRQRFS
                     VRYGEMLF"
     gene            complement(2221..3518)
                     /gene="aceA"
                     /locus_tag="ACHURJ_003551"
                     /pseudo
     CDS             complement(2221..3518)
                     /gene="aceA"
                     /locus_tag="ACHURJ_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
     gene            complement(3548..5149)
                     /gene="aceB"
                     /locus_tag="ACHURJ_003552"
     CDS             complement(3548..5149)
                     /gene="aceB"
                     /locus_tag="ACHURJ_003552"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HFR1282345.1"
                     /translation="MTEQATTTDELAFTRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASICDADWKIRGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEEHNNQVLNKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKMLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFGDAA
                     RLMEQITTSDELIDFLTLPGYCLLA"
     gene            complement(5418..6347)
                     /gene="metA"
                     /locus_tag="ACHURJ_003553"
     CDS             complement(5418..6347)
                     /gene="metA"
                     /locus_tag="ACHURJ_003553"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFR1282346.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPEVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            6504..6951
                     /locus_tag="ACHURJ_003554"
                     /pseudo
     CDS             6504..6951
                     /locus_tag="ACHURJ_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
BASE COUNT         1680 a         1796 c         1816 g         1702 t
ORIGIN      
        1 ccatgcacgc ctgtttggat ttaccgctga agatattatg gatttttggc aacacaaagc
       61 gccacaaaaa tactctgcct ttgagttggc ttttgaattt ggtcaccggg ttattgctga
      121 attaatcctt aatacattaa ataagatggc tgaaagcttt ggctttacgg ataaccctcg
      181 atacattgcg gagaaaaatt atatggaagc tttactcaaa aaagcatctc cccataccgt
      241 acgctaaatc tttgcctgcg ccgatacgca taaacatctt ccacatgccc ttcacgtatg
      301 cggttttgta gtgcgcgcca gtaatcagcg cggaacaggt cggcgtgcat ctcttcaaac
      361 agcggaccaa tacgcgggtc ggcgcatagc cagtggcgaa actcttccgg gaaaacatcg
      421 cccggcgaga cgctgtacca cggttcgctg gcaagttcgt cttccggata gcgcggcggc
      481 gggatgtcgc ggaaattcac ttccgtcatg tagcaaattt catcgtaatc ataaaaaacc
      541 acacgcccgt gacgggtgac accgaagttt ttaaacagca tgtcgccagg aaaaatgtta
      601 gcagcggcaa gctggcgaat agcgttaccg tattcttcaa tggcgtcgcg caactgctga
      661 ccttccactt gttccagcca gatattgagc ggcaccatcc gccgctcgat atagagatgg
      721 cgaattacaa tctgctcacc aagatcggtg attttttccg ctgcttcctg gagcagtaat
      781 tccattaatg ccggggaaat atgccgcttc tccagcacaa agttttcaaa ctcctgggtg
      841 tccgccattc ggcccacacg gtcgtgctct ttcaccagtt gatagcaggc acgaacgtga
      901 gcggcagaca tctctttctg cggcgcgaac ttgtctttga tgactttgaa tacccgatca
      961 aagcccggca gcgtaaacac caacatcacc attccacgaa tacccggcgc ttcaatgaac
     1021 tgctcattac agccctgtag ataaacgaga tattcgcggt agctttcggt tttggcgtgc
     1081 ttctggcagc cgatagccat atacaattca gcagtggttt tacctggcag aatttcccgt
     1141 agccactcga ccagcgctgc ataaaccata aaataagaac gcgcaaagcc aaaaacaatg
     1201 ctcgcttcgg cggtcgtcgt caggcaggta tcaataaata actcgccgtc gtccgtctgg
     1261 tggatcggca gtaagaaggg caacgtgccg gaaggcgtga tcagtttgcc caccagccag
     1321 gcggctttat tgcggtaaaa cagttcgttc gccacctgta aatgactttc cgcgaggttg
     1381 tctgtcccca gcgtttccgt cagatggcga atgatgtaat gaatgtcacg gcttttattc
     1441 tgccagcgca ggcgcagcgg caggtcgctg ataacgcgca tcagtagaga ttcccagccg
     1501 tgatcggggt gaaagtcttt cgccagcggg cggggaatgg tacgaaagcg gcgctctggc
     1561 tgagagctaa agataaaaag ccgctcggga gtaagcgagc ggtggtcaaa taaccgacag
     1621 tacacggagt taaaaaagct ctccgcaatc tcgaagcgcg ggtaatccgg caacagccgc
     1681 gtgtaatgct ctttaacgcg cagtaaaaat gccgcgtccg tactttggcc gttagtaatg
     1741 cagcgcagtt gctccacgac cagaccaacg tgatgatcgt aaagatggat acggtttttc
     1801 atcgccagct ggacagcatg ccagtcggcc tgttcgaaac gctgctgcgc cccggaagtc
     1861 acttcgagga atcgaccata ctgagcgtcg aagccttgca aaatggtttg agcaatcagt
     1921 aattccaggc cacgcggcat ttagcgccct catcaggagc agagaattgc cggatgcggc
     1981 gtaaacgcct tatccagcct acgttcggca acggttgtag gcatgataag acgcgcaagc
     2041 gtcgcatcag gcatcgtgca ccgattgccg gatgcggcgt aaatgcctta tccggcctac
     2101 gttcggcaac ggctgtaggc atgataagac gcgcaagcgt cgcatcaggc atcgtgcacc
     2161 gattgccgga tgcggcgtga acgccttatc cggcctacag tcagcaacgg ttgttgttgc
     2221 ttagaactgc gattcttcag tggagccggt cagcgcggtg actgaagacg tgccgccctg
     2281 aataatagtc gtcactttat cgaagtaacc tgtacccact tcctgctggt gagatacgaa
     2341 ggtatagcca tctttcgcgg cggcaaattc cggctgctgc actttctcaa cgtagtgctt
     2401 cataccctcg ccctggtcat aggcgtttgc caggtcaaac atgttgaacc acatgctgtg
     2461 gatacctgcc agggtgatga actggaactt gtagcccata tccgacagct gctgctggaa
     2521 gctggcaata gttttgtcgt cgaggttttt ctgccagttg aacgacggcg agcagttata
     2581 agccagcagt ttgcccggat atttcgcgtg gatagcttgt gcaaagcgac gcgccagttc
     2641 cagatccggc gtggaggttt cacaccagac caggtcagca tatggcgcat acgccaggcc
     2701 acggctgatc gcttgctcaa tgcccgcatg agtacggaag aagccttcac tggtacgctc
     2761 gccggtaata aattcgctgt catacgggtc gcaatcggag gtgatcagat ccgcggcatc
     2821 ggtacgagca accagtaggg ttggaacgcc cgtcacgtca gctgccagac gcgccgcgac
     2881 cagtttctga atagcttctt gagttggcac taaaactttg ccgcccatgt gaccgcattt
     2941 cttcactgac gccagctgat cttcgaagtg aactgccgct gcaccggctt caatcatcgc
     3001 tttcatcagt tcaaaggcat tcaggacacc gccaaaaccg gcttccgcat cggcaacgat
     3061 cggcaggaag taatcgacat agcgcggatc gcccggctca atgcccgcgg accattggat
     3121 ctgatccgca cggcggaaag tattgttgat ccgctccacc acagctggca ccgagtttgc
     3181 cggatagagc gactgatccg gatacatgct ggccgccagg ttagcgtccg ccgctacctg
     3241 ccatcccgac agatagactg cttcaatacc cgctttcgcc tgttgcagcg cctgaccgcc
     3301 agtcagtgca ccgaggctgt tgatgtagct tttttcgact cgccatgcag cagacgccac
     3361 attctcgccg cgcccagttg cgccagggtg cattcaggat tgactgaatc gcgtaatttc
     3421 accacatctt ccgcgctgta tgggcgagta atgccttccc aacgcggttg agtccactct
     3481 ttctgtaatt cttcaatttg ttgtgtacgg gttttcatgt gcagatgctc catagttatg
     3541 tggtggttta cgctaacagg cagtagcctg gcagggtcag gaaatcaatt aactcatcgg
     3601 aagtggtgat ctgttccatc aagcgtgcgg catcgccaaa acgcccctgg gagaaacgtt
     3661 cttcgcccag ttcgctggca atgactttca tctcttcgcc cagcatctgg cggaacaagg
     3721 ctttagttac cggtttgcca ttgctcaaca ttttttgatg atggatccac tgccagattg
     3781 aggtacggga aatttcagcc gtcgccgcat cttccatcag gccataaatc ggcacacagc
     3841 cgttgccaga gatccacgct tcgatgtact gcacggctac gcgaatattt gcgcgcatac
     3901 cttcttcggt gcgttcaccg tcacaaggtg ccagtagctg atcggcagta atcggcgcgt
     3961 cttgttcgcg catcacttca agctgatttt tacgggagcc gagaatgtcg ttgaataccg
     4021 ccattgccgt atccgcaagg cctgggtgag cgatccatgt gccatcgtga ccgttattgg
     4081 cttccagcga tttatccgct tttactttgt tgagcacctg gttattgtgc tcttcatctt
     4141 tgctcggaat aaacgccgcc atgccgccca tcgcaaaagc accgcgttta tggcaggttt
     4201 taatcaacag gcgtgagtaa gcattcagga atggtttatc catcgtcact gcctgtctgt
     4261 ctggcaggac gcgatcggga tagtttttca acgttttgat atagctgaag atgtaatccc
     4321 aacgaccgca gttcagacca acaatatggt cacgcagcgc gtgaaggatt tcatccatct
     4381 ggaacacggc gggcagcgtt tcaatcagca acgtcgcctt gatggtgccg cgcggcagat
     4441 taaagcgatc ttctgcatag ctgaagactt cgctccacca ggccgcttcc tgccaggact
     4501 gggttttcgg cagatagaaa tagggaccac tgccctttgc caacagagcc tgatagttgt
     4561 ggaagaaata gagcgcaaaa tcaaacaggc tgccggggat tgcctcacca cgccaggtga
     4621 catgtttttc cggcaagtgc agaccgcgta cccgacaaat caaaaccgct ggattgggct
     4681 tgagctggta aattttgcct gcttcattgg tatagctgat ggtaccgtta actgcatcac
     4741 gcaggttaat ttgcccgtcg atcactttgt tccagtccgg ggccagtgaa tcttcgaaat
     4801 cggccataaa gactttcaca ttggcgttga gcgcgttgat caccatcttg cgctctaccg
     4861 ggccagttat ctctacgcgg cggtcttcta agtccgcagg aatcccgcga attttccaat
     4921 cagcatcgca aatggaagct gtttccgaaa taaaatcagg caacgttccg ttatcaatat
     4981 cttgctgctg ctgaatgcgc gctgccagaa gtttatttcg ttgtggcgta aaatgcgtca
     5041 ccagctcagt cagaaattct accgcttcgg cagtaagaat ttgcttctcc tgctcgccat
     5101 acggccttgt gaaagccagt tcatcggttg ttgttgcctg ttcagtcatc gtgcagctcc
     5161 tcgtcatgga tccgaaaact tccccactgt gaacgaagga tcgttgtgcg cttttcgttc
     5221 agcacaacta agactactca tttaaaatgc aaaatcaaaa acaatttcca ttttaattga
     5281 aaatgcttga taacttattg ataacaatgt gaataaaatt tattcacctc ctgaggtgcg
     5341 tttcggaaat aaaaaaggca cccgaaggtg cctgaggtaa ggtgctgaat cgcttaacga
     5401 tcgactatca cagaagatta atccagcgtt ggattcatgt gccgtagatc gtatggcgtg
     5461 atctgataga cgtaatagtt gagccagttg gtaaacagca aattaccgtg actacgccag
     5521 ctcgctcgcg gtgtattttg cggatcattg tgcgggaaat agttatacgg tacttccggg
     5581 tctagtccgg cttccacatc gcggaaatat tcctgcgcca gcgtttgcgc atcatattcg
     5641 ggatggcccg tcacaaaggc aatgcgctta tctttgctgg caaacagata tgcatcccct
     5701 tcttccgtct ctgccagaat ttccagatcg gtgtaatcac gaatcaacgc tgccggaaag
     5761 tcagcatagc gcgaatgtgg tgccaggaat gaatcatcaa agccacgcgt cagaagcgca
     5821 tgaggatgga gaatatgatg ctcgtaaacg ccagagagtt tgtcggtgcg agtttgctta
     5881 ggaatgccgt agaggatatt gagcgcggcc tgtaccgccc agcagacaaa cagcgtcgag
     5941 gtgacgtgat ctttcgacca ctccagcacc tgtttgatct gcggccagta agcgacatca
     6001 ttaaactcca ccaggcccag cggcgcaccg gttacaatca gaccgtcaaa gttctgttcc
     6061 tgaatatctt caaagttaca gtagaagttg ttcagatgct ctgcgggcgt gttgcgcgat
     6121 tcacgggaat cgatgcgcaa cagctgaata tcgacctgca aaggtgagtt tgaaagcagg
     6181 cgcagaaact gattttcagt ttcaatcttc ttcggcatca ggttaaggat cagaacctta
     6241 agtggacgaa tttcctgacc agacgcacga gaagttgtca tcacaaagac gttttcttca
     6301 cgcaagaaat tgacggcggg tagctcgtcc ggcacacgaa tcggcataac ctgattacct
     6361 cactacatac acttatacgt ttagacatcc agatagctga agataaccag aaaatttgcc
     6421 aatgtcgagc ctgcatgttg aaggtgagaa agtttcaagg aaaagactac gttagaatat
     6481 aagaatgact aaaaggagag aaaatggtta ttagtattcg ccgctcacgg catgaggaag
     6541 gggaggaact cgttgcgatt tggtgtcgat ctgtcgatgc cacacacgat tttttatcag
     6601 cagagtatcg ggccgagctg gaggtgctag ctagttcgtt ccttcctgcc ggaagcgccg
     6661 ttgtggatcg cggttaatga acgggatcag ccggttgggt ttatgttgtt aagtgggcag
     6721 catatggatg cgctgtttat cgatcctgat gtgtgcggct gcggcgtggg tcggatgctg
     6781 gtggagcatg cgctctcgat ggcaccggaa ctgacaacca acgttaatga gcaaaatgag
     6841 caggcggttg ggttctataa gaaggtgggt tttaaggtta cgggacgctc tgaggtggac
     6901 gatttgggga aaccgtatcc gttgctgaat ctggcgtatg tgggggaata agccgcccct
     6961 gccgtgcctg cccaggctgg agaagacgcg ccag
//
LOCUS       DAZPEB010000193         6815 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZPEB010000193 DAZPEB010000000
VERSION     DAZPEB010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6815
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.198"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..298
                     /locus_tag="ACHURJ_003555"
     CDS             <1..298
                     /locus_tag="ACHURJ_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFR1282347.1"
                     /translation="LPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVT
                     FANVFTIIPPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(449..3772)
                     /gene="mscM"
                     /locus_tag="ACHURJ_003556"
     CDS             complement(449..3772)
                     /gene="mscM"
                     /locus_tag="ACHURJ_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFR1282348.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERTREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(3794..4762)
                     /gene="asd"
                     /locus_tag="ACHURJ_003557"
     CDS             complement(3794..4762)
                     /gene="asd"
                     /locus_tag="ACHURJ_003557"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFR1282349.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(4859..5911)
                     /gene="rsgA"
                     /locus_tag="ACHURJ_003558"
     CDS             complement(4859..5911)
                     /gene="rsgA"
                     /locus_tag="ACHURJ_003558"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFR1282350.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNELLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            6006..6551
                     /gene="orn"
                     /locus_tag="ACHURJ_003559"
     CDS             6006..6551
                     /gene="orn"
                     /locus_tag="ACHURJ_003559"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFR1282351.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDSFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            complement(6564..>6815)
                     /locus_tag="ACHURJ_003560"
     CDS             complement(6564..>6815)
                     /locus_tag="ACHURJ_003560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282352.1"
                     /translation="TRDPDLGKVVLYQLSYSRVLLNFAFVIFAFLRRYGRRIIREIFP
                     SATPQKSFFCVFISSAEIISKTFNLASKSRRRKWIPALT"
BASE COUNT         1574 a         1830 c         1834 g         1577 t
ORIGIN      
        1 gcttccttac ctgttcctcg cgctggcttt cccattcttt aaagcacgtc aggatctcga
       61 cagaccattt gtgattttca aaacgcgtat gtcggcaatg attgcgacgg tggttgtcgt
      121 actggtggtg acatttgcga acgtcttcac cattattccg cctgtggttg aagccggaga
      181 ctgggacagc acattgtgga tgattggcgg ccctgtcttc ttctcgctgt tagcgatggc
      241 gatttaccag aactattgca gtcgcatggc gaataaacct gagttagctc tcgactgatc
      301 atatgaaatc ctgcaactca tcgtctctcc ggcgatgagt tgtcaggaag taatgcgtat
      361 ggatgatact ctccagggcc ggtgataact gaagatgatg ttaagctgct aaatgagcca
      421 cgttgacatc atttggcagc ttcatgcatt acaaacttcc cgcctgacga cctttgcccg
      481 cagacgtcag cgttctcccc gtttgtttac cgttgaggct ttccagacgc atctggaagg
      541 gcgggaatgg catatcgata ccgtgggcat ggaagccagc cagaatcagc tggtggatct
      601 catggcgtag cggcatacgg tgacccatct cagcggcata aatacgcagc tcgaaaatct
      661 gaatcccttg ttgcagatcc accaggaaga cttccggtgc cgggttgtcg atcaccagcg
      721 agcagcgacg cgctgcggtg agcaggattt ccgtcacttc ttcactgttg gcatcagcag
      781 gggccggaat cgtcaacact acgcgcgtaa ccgagtcaga gagcgaccag ttgataaact
      841 gctcggtaat aaacgccttg ttcggcacga ttatctcttt acggtcccag tcgctgatgg
      901 tggtggcgcg ggtgttaatt ttcgtcacgc taccggtgag atcgcgaatt gtcaccgtat
      961 cgccaatgcg aatcggtttt tcgaacagga taatcagacc agagataaag ttggcgaaaa
     1021 tttcctgcaa accaaaaccg agaccaacac cgagcgcggc gaccagccac tgcaatttcg
     1081 accattcaat accaatcatc gagaagccga ccagcccgcc aatcagcatc agcagatatt
     1141 tggtgatggt ggtgatggcg tagcctgtac ccggcgttaa atccaggtgc tgcaaaatcg
     1201 ccagttccag cagcgcgggc aggttgcgca ccagctgcgt ggtgatgata aacaccagaa
     1261 tggcaatcag caccgcaccg agggtaattg gctccagact ttctacgcct tgtaccgtgg
     1321 aggtgacatc ccacagcgaa atattttcga ggaagccgaa agcggaatgg atttctgacc
     1381 acagcacaat gacagaaagc agggcgatca acatcaaaat tgagcgcacc agccgcaagg
     1441 attgcgcact gatggcatcg agatcgactt cgctttcatc gacttcaatt gctccttccg
     1501 ggctactgtg atgatgcgct tcctcttcgc cacgcgcacg ttgcgctaac atctctgccc
     1561 ggcgatgctt cgcccggtca aacgccagcc tgcggcgctg gatcagcatc cagcggcgga
     1621 taacgtgata aaccaccagt agcaggaacc agatggcaac cgaggtttca agcctcgcta
     1681 acagcgcctg tgccgttgcc agataaccca cagccgacgc cagaatggca accaacggcg
     1741 cgccaatcat catgttccac agcatatggt tggtaatgtt gtcgccgctg ccctctttgt
     1801 tgagatacag tgggatcccg gcctttttca ggctgagggt gaccaccgcc agcgcaccgc
     1861 aaatgaggat aaagcaaagc cgtcccagcg aaccggagaa ttcacggtcg tcgaggttat
     1921 cgaacatcat cagcgccata atcagcggca caataagccc gatgctcatc aggtagtagc
     1981 gcatcccacg ggaaacacgt tcgcgcggcc agccaaaatg agcgataaac aagccgttcg
     2041 ggcgggcaaa ggtggcgcaa atcatcacta cccacagcag cggcacggta gccgttacac
     2101 catcgccaat cgcgaccgcc agcggataag gccacgcctc gcgcaagccg taacccagcg
     2161 tcatccacag caccggtaac ggtgacgcga cgagaatcga ccagaaaaga gtgcgcaacg
     2221 tcagccagaa gtgatcctga gtcactttgc caactttcgc cgccgaacgt tcaagaaaac
     2281 gggtgaaata gcggcgcgag taaatactgc aaccgaccag aatcaacgcg ccaaacagcg
     2341 gcaaaattgt ctctttgctg gtcagcatca tcacactggc tttgcccaac tgactgaagg
     2401 tgtccagcga aatgagacga cgcagatcct gggcgatttc cagcggccag gcgatggtca
     2461 tcgggcgcac gtcagaggtc cagaacagat agcggtgcgt tgcttcgttc acctctttca
     2521 gcgcatcctc cagttgcccg ttggagactt tcagcttggt cagttccaat agtagcgtgt
     2581 cgccaccctg caacaatgag ttcagcaact cacgctgagt gcgcagttgt gcttccagaa
     2641 tacggttttg ctcggcggtc agcggctgac cgtcggcctg atggatttgc cgtagcagcg
     2701 gctgtttatt aagcagatcc tcataacgta accgttgcac acgcaactgc gccatttcgg
     2761 tatcaagctg ttgtggtttt ggcatttctg gcagccgtgc cacctgcgcc cgcagcgctt
     2821 cgccgagcag attggacgat cccagccatt gcgactgttc acgcagcgta ttcaacgcct
     2881 gccggacctg taacgtctgg ctggcagcct gacgctgttg cgaggcaacg agatccatcc
     2941 gctgcgcctg ttgattcaaa gccgccgata gttcgcggtt aattttgaat tgcgcgacga
     3001 tatctttcgg caaatcggcg ctgttttctg ccagcaattc ggtactttcc agcgcccgct
     3061 ccgcctcaag ctgacgttgg ctgtttaatt gattacgcaa ggcctgcaaa tacgcatcca
     3121 gttgctggct ctctttttcc gccagctctg agcgtaagcg cgctaattcc tggcggttat
     3181 tggcagacag ctgcgccagc tccagttcat caacgagcgc cttaagacgt gcagagtcag
     3241 actgcaacgc gaaattttgt gcctgattga gcggagtatt gccggtaagc gttcccaggc
     3301 ggcgctcgat ctcatttaac tgacggcggg cgtcggtttg ctgttgcggc agttgattca
     3361 gcgaatcggc aatctcgcgg gtgcgctcct gctcttgctg ggcctgacgg cttttatcca
     3421 gcaactggct gctgacctgg agaatttcct gattcagcgc gtcggtagac attcccggcg
     3481 acacgctgcg cggctcgtca cgcatgttgt ttaattgtgc gcgcagagta gcggagagtt
     3541 tcggataatt atcgataact tgctgatatt gtttgatgcg ctcaagggaa ccttttcgtt
     3601 cctcaagcgc atttaaggca gactggagcg cctctacgac ttccggctgt gcgggtttcg
     3661 ccgcttttgc ctgctccagt tcctgagtga tttgtttgct atcgggggcc gtcgcggcgt
     3721 acgcccccca actgaggcac caggccatca gaaaagtgat aatcaggcgc acgtcagcgt
     3781 ttcctttgat ggattagacc tggtcttttt tatcgtcaac caatgggctg gcgtcgtgtt
     3841 ctgcttcgat ctcttcagca ggaagtgggg cgggttcagc gtctggcgta acaaaggttt
     3901 cggtagatac tgccagcggc tggccaattt tcgtgacaga caggctttcc agttgctcaa
     3961 ccagattcac tttacccggt gcaaacaggt tgataacggt ggaaccgagt ttaaagcgac
     4021 ccatttcctg gcctttcagc agtgccacag aaccgtcgtt ttccccggca ggccaggtcc
     4081 agcgcttgat gataccttcg cgcggcggcg taatggtgcc cgcccagacc gtctcaatgc
     4141 tgccaacaat cgtcgctccg accagaatct gcgccattgg gccaaattcg gtatcgaaaa
     4201 ggcaaatcac gcgttcgtta cgggcaaaca gattcggcac gttctgagcg gtgagatggt
     4261 taacggagaa gagatcgccc ggcacgtaga tcatctcacg cagaataccg ttgcacggca
     4321 tgtgtacgcg atggtagtca cgcggggaga ggtaagtggt cacaaacgta ccgttgcgga
     4381 acaggtccgc catcagatag ttgcctgcca gcagggcttc gaggctgtag ttgtggcctt
     4441 tggcttgcag gattttatct tcttcgattt tacccaactg gctgataacg ccatcggcag
     4501 gcatgaccag tacattcgga tcggtatcga ttgggcgtac ttcgtcacgc agcggacgga
     4561 caaagaattc gttaaaggtg cggtagctgg cggtgtccgg cttttgcgcc tctttcatgt
     4621 cgaccttgta gtatttaacg aacagatcga taaccagttt tgtcagccat cctgcccgct
     4681 tgcttgcgcc ccaacccgcc aggcgagtaa gccatagttt cggcagaatg tactgtagcg
     4741 aaagtttaaa tgaatttaac aaggtagcct ccaggccatt gttttgtcgt tcctgatccg
     4801 gccaacatgc cggatcctga aaaaaagggg acgattctaa cgacggttag cttaattgtc
     4861 agtcatccgt atcagaaaag tttttacgcg tttttacctg cgccatgctt tccagaatac
     4921 ggtgatagtt ttcgaaacgg gtttccgcga ttttcccttc ctcaaccgct tcccggatag
     4981 cgcagcccgg atcggtatcg tgtttgcaat cgcgatattt acacagacct aaatagtcat
     5041 ggaattcgac aaagccctga gtgatttgtt ccggctccag gtgccagagg ccgaactcac
     5101 gcacaccagg ggaatcaatc acatcaccgc cgtgcgggaa gtgatacagc cgagcggcgg
     5161 tcgtggtgtg ctgaccgagc cccgagttgt cagagacatc gttggtcagg atctcttttt
     5221 gcagccccag caactcattc agcaggctgg atttgccgac gccagactgc ccggcaaaaa
     5281 tgctgatgcg cccggtcaac gcctcttcca gcggttttag cccatcctga gtatggctgg
     5341 aaaccatcaa tacgcgataa ccgatattgc ggtagatatc catctgctcg ttgacgaacg
     5401 ccatgccttc gtcgtccagc aggtctatct tgttgagcac aataatcggc tcaatctgca
     5461 aggtttcgca ggccaccagg taacggtcga taatattgag tgacagctcc ggcaaaatgg
     5521 cggagacaat gacaatctgg tcgatgttgg cggcaatagg ttttacgccg tcgtagaaat
     5581 ccggacgcgt caacaccgag gtacgctcat gcaccgcttc cacgatccct ttgacattta
     5641 cgccttccgc cgccggttta cccggacgcc agactacacg gtcgccggtc accagcgaac
     5701 ggatggtacg gcgaatattg cagcggtgaa cgtcgccatc ggcggattcc acatcagcgt
     5761 gcataccaaa gcggctgatg acgatacctt catcaggctc gccaaacaga ttgtcgtcgt
     5821 agtcgggctt ctccttagac gttttaagac gacgctggtg attggcgttc acgcggcgct
     5881 gctggccttt ggagagttta tttttactca atcgtacaga ctcctggtcg cccctgatgg
     5941 gcaaaacatc tatgatacac gcaattgtgg atcaatatag tcactgtgaa tgggtggaaa
     6001 atagcatgag tgccaatgaa aacaacctga tttggatcga tcttgagatg actggtctgg
     6061 atcccgagcg cgatcgcatt attgagattg ccacgctggt gaccgatgcc aacctgaata
     6121 ttctggcaga agggccgacc attgcagtac atcagtccga tgaacagctg gcgctgatgg
     6181 atgactggaa cgtgcgcacc cataccgcca gcgggctggt agagcgcgtg aaagcgagca
     6241 cgatgggcga tcgggaagct gaactggcaa cgctcgaatt cttaaaacag tgggtgcctg
     6301 cgggaaaatc gccgatttgc ggtaacagca tcggacagga ccgtcgtttc ctgtttaaat
     6361 acatgccgga gctggaagcc tacttccact accgttatct cgatgtcagc accctgaaag
     6421 agctggcgcg ccgctggaag ccggaaattc tggatagttt taccaagcag gggacgcatc
     6481 aggcgatgga tgatatccgt gaatcggtgg cggagctggc ttactaccgc gagcatttta
     6541 tcaagctgta aaatttttcc tggtcacgta agcgccggaa tccacttgcg gcggcgtgat
     6601 tttgacgcta aattgaacgt cttgctgata atttcagcac ttgagataaa aacgcaaaaa
     6661 aaactttttt ggggggttgc agagggaaag atttctcgta taatacgcct cccgtaacga
     6721 cgcagaaatg cgaaaattac gaaagcaaaa ttaagtagta cgcgggaata gctcagttgg
     6781 tagagcacga ccttgccaag gtcggggtcg cgagt
//
LOCUS       DAZPEB010000194         6800 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZPEB010000194 DAZPEB010000000
VERSION     DAZPEB010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6800
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.199"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            186..2102
                     /gene="dnaK"
                     /locus_tag="ACHURJ_003561"
     CDS             186..2102
                     /gene="dnaK"
                     /locus_tag="ACHURJ_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFR1282353.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            2191..3321
                     /gene="dnaJ"
                     /locus_tag="ACHURJ_003562"
     CDS             2191..3321
                     /gene="dnaJ"
                     /locus_tag="ACHURJ_003562"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFR1282354.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGQHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(3425..3577)
                     /gene="mokC"
                     /locus_tag="ACHURJ_003563"
     CDS             complement(3425..3577)
                     /gene="mokC"
                     /locus_tag="ACHURJ_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFR1282355.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            4163..5329
                     /gene="nhaA"
                     /locus_tag="ACHURJ_003564"
     CDS             4163..5329
                     /gene="nhaA"
                     /locus_tag="ACHURJ_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFR1282356.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLSEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            5395..6294
                     /gene="nhaR"
                     /locus_tag="ACHURJ_003565"
     CDS             5395..6294
                     /gene="nhaR"
                     /locus_tag="ACHURJ_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFR1282357.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(6332..>6800)
                     /locus_tag="ACHURJ_003566"
     CDS             complement(6332..>6800)
                     /locus_tag="ACHURJ_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFR1282358.1"
                     /translation="RFNYNLNTILLVNLMTCGFEDQSIDTGRFVLSRINNENYQFHHI
                     RFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKNELENSEDGAGQVGFQLSNDG
                     TNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
BASE COUNT         1738 a         1600 c         1757 g         1705 t
ORIGIN      
        1 ctgcgcaaaa gcacaaaaaa tttttgcatc tcccccttga tgacgtgggt tacgacccca
       61 tttagtagtc aaccgcagtg agtgagtctg caaaaaaatg aaattgggca gttgaaacca
      121 gacatttcgc ccctattaca gactcacaac cacatgatga ccgaatatat agtggagacg
      181 tttagatggg taaaataatt ggtatcgacc tgggtactac caactcttgt gtagcgatta
      241 tggatggcac cactcctcgc gtgctggaga acgccgaagg cgatcgcacc acgccttcta
      301 ttattgccta tacccaggat ggtgaaactc tggttggtca gccggctaaa cgtcaggcag
      361 tgacgaaccc gcaaaacacc ctgtttgcga ttaaacgcct gattggccgc cgcttccagg
      421 acgaagaagt acagcgtgat gtttccatca tgccgttcaa aattattgct gctgataacg
      481 gcgacgcatg ggtcgaagtt aaaggccaga aaatggcacc gccgcagatt tctgctgaag
      541 tgctgaaaaa aatgaagaaa accgctgaag attacttggg tgaaccggta actgaagctg
      601 ttatcaccgt accggcatac tttaacgatg ctcagcgtca ggcaaccaaa gacgcaggcc
      661 gtatcgctgg tctggaagta aaacgtatca tcaacgaacc gaccgcagct gcgctggctt
      721 acggtctgga caaaggtact ggcaaccgta ctatcgcggt ttatgacctg gggggtggta
      781 ctttcgatat ttctattatc gaaatcgacg aagttgatgg cgaaaaaacc ttcgaagttc
      841 tggctaccaa cggtgatacc cacctgggtg gtgaagactt cgacagccgt ctgatcaact
      901 acctggttga agaattcaag aaagatcagg gcattgacct gcgcaacgat ccgctggcaa
      961 tgcagcgcct gaaagaagcg gcagaaaaag cgaaaatcga actgtcttcc gctcagcaga
     1021 ccgacgttaa cctgccgtac atcactgcag atgcgaccgg tccgaaacac atgaacatca
     1081 aagtgactcg tgcgaaactg gaaagcttgg ttgaagatct ggtaaaccgt tccattgagc
     1141 cgctgaaagt tgcactgcag gacgctggcc tgtccgtatc tgatatcgac gacgttatcc
     1201 tcgttggtgg tcagactcgt atgccaatgg ttcagaagaa agttgctgag ttctttggta
     1261 aagagccgcg taaagacgtt aacccggacg aagctgtagc aatcggtgct gctgttcagg
     1321 gtggtgttct gactggtgac gtgaaagacg tactgctgct ggacgttacc ccgctgtctc
     1381 tgggtatcga aaccatgggc ggtgtgatga cgacgctgat cgcgaaaaac accactatcc
     1441 cgaccaagca cagccaggtg ttctctaccg ctgaagacaa ccagtctgcg gtaaccatcc
     1501 atgtgctgca gggtgaacgt aaacgtgcgg ctgataacaa atctctgggt cagttcaacc
     1561 tggatggtat caacccggca ccgcgcggca tgccgcagat cgaagttacc ttcgatatcg
     1621 atgctgacgg tatcctgcac gtttccgcga aagataaaaa cagcggtaaa gagcagaaga
     1681 tcaccatcaa ggcttcttct ggtctgaacg aagatgaaat ccagaaaatg gtacgcgacg
     1741 cagaagctaa cgccgaagct gaccgtaagt ttgaagagct ggtacagact cgcaaccagg
     1801 gcgaccatct gctgcacagc acccgtaagc aggttgaaga agcaggcgac aaactgccgg
     1861 ctgacgacaa aactgctatc gagtctgcac tgactgcact ggaaactgct ctgaaaggtg
     1921 aagacaaagc cgctatcgaa gcgaaaatgc aggagctggc acaggtttcc cagaaactga
     1981 tggaaatcgc ccagcagcaa catgcccagc agcagactgc cggtgctgat gcttctgcaa
     2041 acaacgcgaa agatgacgat gttgtcgacg ctgaatttga agaagtcaaa gacaaaaaat
     2101 aatcgcccta taaacgggta attatactga cacgggcgaa ggggaatttc ctctccgccc
     2161 gtgcattcat ctaggggcaa tttaaaaaag atggctaagc aagattatta cgagatttta
     2221 ggcgtttcca aaacagcgga agagcgtgaa atcaaaaagg cctacaaacg cctggccatg
     2281 aaataccacc cggaccgtaa ccagggtgac aaagaggccg aggcgaaatt taaagagatc
     2341 aaggaagctt atgaagttct gaccgactcg caaaaacgtg cggcatacga tcagtatggt
     2401 catgctgcgt ttgagcaagg cggcatgggc ggcggcggtt ttggcggcgg cgcagacttc
     2461 agcgatattt ttggtgacgt tttcggcgat atttttggcg gcggacgtgg tcgtcaacgt
     2521 gcggcgcgcg gtgctgattt acgctataac atggagctca ccctcgaaga agctgtacgt
     2581 ggcgtgacca aagagatccg cattccgact ctggaagagt gtgacgtttg ccacggtagc
     2641 ggtgcaaaac caggtacaca gccgcagacc tgtccgacct gtcatggttc tggtcaggtg
     2701 cagatgcgtc agggtttctt tgccgtgcag cagacctgtc cacactgtca gggccgcggt
     2761 acgctgatca aagatccgtg caacaaatgt catggtcatg gtcgtgttga gcgcagcaaa
     2821 acgctgtccg ttaaaatccc ggcaggggtg gacactggag accgcatccg tcttgcgggc
     2881 gaaggtgaag cgggccagca tggcgcaccg gcaggcgatc tgtacgttca ggttcaggtt
     2941 aaacagcacc cgattttcga gcgtgaaggc aacaacctgt attgcgaagt cccgatcaac
     3001 ttcgctatgg cggcgctggg tggcgaaatc gaagtaccga cccttgatgg tcgcgtcaaa
     3061 ctgaaagtgc ctggcgaaac ccagaccggt aagcttttcc gtatgcgcgg taaaggcgtc
     3121 aagtctgtcc gcggtggcgc acagggtgat ttgctgtgcc gcgttgtcgt cgaaacaccg
     3181 gtaggcctga acgagaagca gaaacagctg ctgcaagagc tgcaagaaag cttcggtggc
     3241 ccaaccggcg agcacaacag cccgcgctca aagagcttct ttgatggtgt gaagaagttt
     3301 tttgacgacc tgacccgcta acctccccaa aagcctgccc gtgggcaggc ctgggtaaaa
     3361 atagggtgcg ttgaagatat gcgagcacct gtaaagtggc ggggatcact ccccgccgtt
     3421 gctcttactc ggattcgtaa gccgtgaaaa cagcaacctc cgtctggcca gttcggatgt
     3481 gaacctcaca gaggtctttt ctcgttacca gcgccgccac tacggcggtg atacagatga
     3541 cgatcagggc gacaatcatc gccttatgct gcttcattgc tctcttctcc ttgaccttac
     3601 ggtcagtaag aggcactcta catgtgttca gcatataggg ggcctcgggt tgatggtaaa
     3661 atatcactcg gggcttttct ctatctgccg ttcagctaat gcctgagaca gacagcctca
     3721 agcacccgcc gctattatat cgctctcttt aacccatttt gttttatcga ttctaatcct
     3781 gaagacgcct cgcatttttg tggcgtaatt ttttaatgat ttaattattt aactttaatt
     3841 tatctcttta tcgcaattat tgacgacaag ctggattatt tttgaaatat tgtcctaaca
     3901 aataacgccg actgacaaga aattaattat tacttttcct aattaatccc tcagaaatcc
     3961 tcaccttaag ctatgattat ctgagcttag ggtcactcgt cagcgcttac agccgtcaaa
     4021 aactcatctc accgctgatg gcgaaaattc tacaaaggct cgtaaaaaac gaattattcc
     4081 tacactataa tctgatttta acgatgattc gtgcggggta aaatagtaaa aacgatttat
     4141 tcacctgaaa gagaaataaa aagtgaaaca tctgcatcga ttctttagca gtgatgcctc
     4201 gggaggcatt attcttatca ttgccgctat cctggcgatg atgatggcca acagcggcgc
     4261 aaccagtgga tggtatcacg actttctgga gacgccggtt cagctccggg ttggttcact
     4321 cgaaatcaac aaaaacatgc tgttatggat aaatgacgcg ctgatggcgg tatttttcct
     4381 gttagtcggt ctggaagtta aacgtgaact gatgcaagga tcgctagcca gcttacgcca
     4441 ggccgcattt ccagttatcg ccgctattgg tgggatgatt gtgccggcat tactctatct
     4501 ggcctttaac tatgccgatc cgattacccg cgaagggtgg gcgatcccgg cggctactga
     4561 cattgccttt gcacttggag tgctggcgct gttgggaagt cgtgttccgt tagcactgaa
     4621 gatctttttg atggctctgg ctattatcga cgatcttggg gccatcatta tcatcgcatt
     4681 gttctacact aatgacttat cgatggcctc tcttggcgtc gcggctgtag caattgcggt
     4741 actcgcggta ttgaatctgt gtggtgtacg ccgcacgggc gtctatattc tggttggcgt
     4801 agtgttgtgg actgcggtgt tgaaatcggg ggttcacgca accttggcgg gggtaattgt
     4861 cggcttcttt attcctttga aagagaagca tgggcgttct ccagcgaagc gactggagca
     4921 tgtgttacac ccgtgggtgg cgtatctgat tttgccgctg tttgcatttg ctaatgctgg
     4981 cgtttcactg caaggcgtca cgctggatgg cttgacctcc attctgccat tggggatcat
     5041 cgctggcttg ctgattggca aaccgctggg gattagtctg ttctgctggt tggcgctgcg
     5101 tttgaaactg gcgcatctgt ctgagggaac gacttatcag caaattatgg cggtggggat
     5161 cctgtgcggt atcggtttta ctatgtctat ctttattgcc agcctggcct ttggtagcgt
     5221 agatccagaa ctgattaact gggcgaaact cggtatcctg gtcggttcta tctcttcggc
     5281 ggtaattgga tacagctggt tacgcgttcg tttgcgtcca tcagtgtgac aggacggttt
     5341 accggggagc cataaacggc tcccttttca ttgttatcag ggagagaaat gagcatgtct
     5401 catatcaatt acaaccactt gtattacttc tggcatgtct ataaagaagg ttccgtggtt
     5461 ggcgcagcgg aggcgcttta tttaactcca caaaccatta ccggacagat ccgggctctg
     5521 gaagagcgcc tgcaaggcaa attatttaag cgcaagggac gtggtctcga acccagcgag
     5581 ctgggagaac tggtctatcg ctatgccgat aaaatgttca ccttaagcca ggaaatgctg
     5641 gatattgtga actatcgcaa agaatccaat ttattgtttg acgttggcgt ggctgatgca
     5701 ctttccaaac gcctggtcag tagcgtgctg aatgctgcag tggtagaagg cgagccgatt
     5761 catcttcgct gcttcgaatc cacccacgaa atgctactgg agcaattaag ccagcataaa
     5821 ctggatatga tcatttccga ctgtccgata gactctacgc agcaggaagg cctgttctcc
     5881 gtgagaattg gcgaatgtgg cgtgagtttc tggtgtacaa atccaccacc agaaaaaccg
     5941 ttcccggctt gtctggaaga acggcgactt ttgattcctg ggcgacgttc aatgttaggg
     6001 cgcaaattgc ttaactggtt taactcccag ggattaaacg tagaaatcct cggcgagttt
     6061 gatgatgccg cgttgatgaa agcttttggt gcaatgcaca atgcaatctt cgttgcccca
     6121 acgctttatg catatgactt ttatgccgat aaaactgtcg tagaaattgg tcgcgtcgag
     6181 aatgtgatgg aagagtacca tgctattttt gctgagcgga tgattcagca cccggcggta
     6241 cagcgaatct gcaatacgga ttattctgcg ctttttagtc cagcggcgcg ttaatcggca
     6301 gctcccccaa agttaaagtg gaggagatag attagttgta cataaccacg attttgactc
     6361 ggctcattat ttgcccgctt gagacattat ttccatatgt acgcgggcga ataaatagag
     6421 gaatctgatt acttccttca tggggatgct gaaaagagta gtaattgctt ttaccatact
     6481 ggatttcatt cgtaccatca ttggagagtt gaaagcctac ttgacccgca ccgtcctcac
     6541 tgttttctaa ctcatttttt aatatgctgg ttcctgactc ctgtatcgtc actggttcaa
     6601 agtaatattc aacatcactg ggttcaaaaa ctaattcacc ttgctcgccc tggatgcagt
     6661 caaatcttat atgatgaaac tgataatttt cattattgat acgagacagt acaaatcgtc
     6721 cagtgtcgat ggattgatct tcaaaaccac aggtcattaa atttaccagt aaaatagtat
     6781 ttaaattata attaaagcgg
//
LOCUS       DAZPEB010000195         6792 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZPEB010000195 DAZPEB010000000
VERSION     DAZPEB010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6792
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.200"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(240..725)
                     /gene="sixA"
                     /locus_tag="ACHURJ_003567"
     CDS             complement(240..725)
                     /gene="sixA"
                     /locus_tag="ACHURJ_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFR1282359.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(928..3072)
                     /gene="fadJ"
                     /locus_tag="ACHURJ_003568"
     CDS             complement(928..3072)
                     /gene="fadJ"
                     /locus_tag="ACHURJ_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFR1282360.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTVQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTTLEMILTGKQLRAKQALKLGLVDDVVPHSILLEVAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTKGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVSERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3072..4382)
                     /gene="fadI"
                     /locus_tag="ACHURJ_003569"
     CDS             complement(3072..4382)
                     /gene="fadI"
                     /locus_tag="ACHURJ_003569"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFR1282361.1"
                     /translation="MGQVLPLVTRQGDRIAIVSCLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLFGPAWSTPLALERAGLTM
                     SDLTLIDMHEAFAAQTLANIQLLGSERFACEVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGAWMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4562..4846)
                     /locus_tag="ACHURJ_003570"
     CDS             complement(4562..4846)
                     /locus_tag="ACHURJ_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFR1282362.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5218..6558
                     /gene="fadL"
                     /locus_tag="ACHURJ_003571"
     CDS             5218..6558
                     /gene="fadL"
                     /locus_tag="ACHURJ_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFR1282363.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGNTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
BASE COUNT         1701 a         1819 c         1611 g         1661 t
ORIGIN      
        1 caactctatt atctgtatta ataaatggca atgagtgtca aacagacatt attcatttca
       61 acatgtaact ttgggcaaaa tcaagatacg tgcagaagaa taatttccga atgaatgtag
      121 aaagtgtttt atttatagat aaggagagtc ttcgatagta gtgaggttga cagatatccg
      181 ctgcctgatg agcaattatg cccaatgaat ctacctcatt gggcataatt tggaacacat
      241 cagatagctt ttgccatctt cagattacac gggctcatct gccagttgaa tgtaccgttt
      301 ccgctttcat caagcgttac gctggcaatg gccgaggtgg taaacatagg cggcgtttcg
      361 cccggacata actcggcaac cagatacccg actaacggta agtgagagat caccagtacc
      421 gaagcaacac cttcattggt caacgcctgc aaataggcac tgaccagacc aacatcgccg
      481 cagggcgtta actccggcag aacttctgca ctggaaggca ggttcagaca atcccctact
      541 tcttccagtg tttgctcggc tcgcaggaac gggctcacca gaacgcgttc gatttccact
      601 ttttgacctt tcagccagtt cgccatcagg cgagattcgt cacaaccatt agtggtcaga
      661 ggacgaaccg aatcactggc ggcatcgagg gctgcgtcgc cgtgacgcat gataaaaact
      721 tgcatattgc accgcttttg ttaaccagat tcgcccattt tctttcatct gaaaccagag
      781 aaagaaaacg gtggccggca ttgtgcctta tccattcacc gaatgaaacg ctgtttttta
      841 cctcaatggc gtaagtatag tcaatatgcg tttacatttt gaccaatact ccgccattca
      901 gcgcggattc atatagcttt gaccttctta ttgcaggtca gttgcagttg ttttccaaaa
      961 actttcccca cgcgcgccca tctcgaccaa acgctcgcaa ggggtaaaac gggaaccata
     1021 ctgcgtggcc agtcgttgca ttattgcaac cacttcgccc gcgccgagag aatcgatata
     1081 gcggaacggt ccaccgagaa atggcggaaa accaatgcca aataccgcac caatatcccc
     1141 gtcacgcacg ctacggataa cctgctcatc aacacaacgt actgcttcat tcagcatcaa
     1201 catcacacac cgttcagaaa cctgcggtgc ggagagtcgc ccctgccctt gtgcgccaat
     1261 gagcgggtaa atggcgggat cgacctgttt tttgctttta cgccctttct gaccataaag
     1321 atagaaaccc cgaccatttt ttctgccttt gcgatcgtcg ttcaaaattg aagaaacaac
     1381 atttgcaggc gcgctaaaac gttctccata agcggcttcc agtacaggaa taattttagt
     1441 cccggtgtcg attcctacct catccaaaag ttggattgga cctaccggaa aaccaaattt
     1501 cactagcgcg gcatcaatgt gctctacccg ttcacctttg gtcagcatgc ggatagcttc
     1561 attaatgtaa ggcgctaaga tgcgattgac gtaaaacccg gctttgtcac gcacgacaat
     1621 gggcgttttg ccctgttttt tcgccagttt taccgtagtg gcgatggttt gcgctgatgt
     1681 acccgcatga ggaataatct ccaccagcgg cattttttcc accggactga agaaatgcag
     1741 gccgataact tgctcaggtc gcgtggcgtg agcggcgata tcaccaatcg gtaaagatga
     1801 cgtattcgaa gcaaagatgg tatgagcggc gcaattttgc tcaacttccg ccaccatctg
     1861 ttgtttcaat tcgagatttt caaacaccgc ttcaataatc agatcgcgat gggcaaagcc
     1921 gcgatagtcc gtcgttccgg agattaatgc cagctgtttg tcacgttcgc tggctttgag
     1981 atgacgacgg cgaactttgc cctccagctg atcccaactg tacttcagcg catgatttat
     2041 gccctgcggg ttgatatctt taattctgac cggaagcccc gctttacaag cagtgacata
     2101 agcaataccg ccgcccatca agccaccacc taaaattccc acgctgttta atggcgcagg
     2161 cggtgcatca ctaccgggat ctttcttcac gtccgtactg gcaaaaaaga tactacgcag
     2221 cgcctgcgat tgtggcgtca tcgccagttc gccaaacgcc cgagcttcgg cgtcataacc
     2281 gctgctggtg ccctgcgcta atcccgtttc aacaacctcc aggatgcgtt ctgtcgccgg
     2341 ataattgcct tgagttttgt gttctgtttt cttgccgacc attttgaaca gcagcgcacg
     2401 acctaacggc cccgccagaa tacgctcgcg tacaggtaga gggcgggaag atgggcgatc
     2461 cttcttcgcc agctcaacag cgacttccag caaaatggag tgcggaacaa cgtcatccac
     2521 cagccccagc tttaatgcct gtttcgcccg aagttgtttt ccggtgagga tcatctctaa
     2581 tgttgtgctg acgcctatca gacgcggtaa acgctgggtg ccgcctgaac cgggtaacaa
     2641 tccaagttgt acttcaggca aaccgagcac cgttttagga tcgtcagtac aaacgcgacc
     2701 gtggcacgcc agcgccaact ccagcccacc acccaggcaa gcgccatgaa tagccgcgat
     2761 aaccggaatg ggcaaagcat gaatctccgc catcaactgt tggccctgcc gcgccagagc
     2821 ttccgcttct tgcaccgttt tgcagttgcc gatcatgttg atgtctgctc cggcgataaa
     2881 attgtccggt ttcgcggaga taaacaccac gccgcgcaac tctttgtttt cacggagttg
     2941 cttaataatg gcgcgcacct gcgaggcaaa ttccgccttc agggtattca ttttctcatc
     3001 cggtacgtcg atggtgatga tagcaatgtt gtccagacga acattaagag taaacgctga
     3061 tgccatttcc attattccgc ctccagaacc attgccgcgc caagcccacc ggcagcacag
     3121 gcggtaacta aaccaaatcc accgccgcga cggcgaagtt catgcaatgt ctgggtaatc
     3181 atccacgcgc cggtcgccgc gaagggatgc ccgtaagcaa tcgaaccgcc aagcacgtta
     3241 aatttgctat cgtccacttc gccagtggca tgtgcacgcc ccagtacttc acaagcaaaa
     3301 cgttcactac ccagcaactg aatattcgcc agtgtctgag ctgcaaaggc ttcgtgcata
     3361 tcgatcaatg tcagatcgct cattgtcaaa ccggcacgtt ccagcgccag cggtgttgac
     3421 caggccggac cgaacaacat gtcctgccag acatcaatcg cagtaaatgc gtagctgcgc
     3481 agatacccca gcggcaccag ccctaattct ttcgcccggg attcagtcat caggatcacc
     3541 gctgccgcgc catcggtcag cggcgtactg tttgccgccg ttaccgttcc gtgtttgcga
     3601 tcaaacgccg ggcgcagctt tgcgtaatcg gcaagcgagg aattaccgcg aatattgttg
     3661 tcttcgacaa gcggttgttt ataaggaggg ataaaggcag tcatcacctc ttctttgagt
     3721 tttccgtctg accatgcctg agcggcacgc tgatgcgaac ggtgcgctaa tgcatcttgc
     3781 tgttctcggg tgatgccgta ggttttcgcc atttgctctg cggtgtcacc catccgcaag
     3841 ccggtagaat attctgctac tgcaggcggt acgggcatta aatcgcgcaa acgcaggcga
     3901 gagaagagtt tcaggcgctg gctcatggtt cgagctttgt tgacatcaac cagcacgcgc
     3961 gccagttttt tactgacgcc aattggcaat acggaagagg aatctgcccc accggcaatc
     4021 cccgctcgga tagttcccgc catcaggctt tctgcgacgt ttgcaaccgc ctggaaactg
     4081 gtagcgcaag cgcggctgac gctgtaagca tcggtatgca cattcattcc ggtaccgaga
     4141 acaatttcac gcgcaatgtt gggggcttca ggcatttgta cgacctgacc aaagaccagc
     4201 tgttcaatca cttcagcggg gatctcgctg cgtgccagca gttcgcctac caccatcttc
     4261 cctaaatcaa ccgcgggaat gccatgaaaa gccgtcgcct ggcgggcaaa aggcgtacgt
     4321 aaacagctaa caatggcgat acgatcgccc tggcgggtaa ccagcggtaa aacctgaccc
     4381 ataacactcc cctgtaaaaa aataaataaa gtggtctgac ctgattatag tcttaaccat
     4441 ttttttacat ttagccaagc ggagaaaaag gaaagtggga gctatgacac agtgaagaca
     4501 ggagaaagaa aagaaaacgc ccctgccagc aactgacagg ggcgaacgta cccgagagga
     4561 attaacgcag acccagctgg aagatcagca tttcagcttc gcaggcaaaa gtgaaatcga
     4621 tatccaggcg cacaccgtca gactcttcag tgaaagtcgg ggagattttg cacggttcgg
     4681 attccacgct acgggctttt tcagtcaacg ccgccagcgt ttgttctgct tctgcgcggt
     4741 ttgcaaacac gcggctgtaa gacgcggtgc agtcggagtt gtccataatg gtgccaacat
     4801 ccatacagca gcaaaccggg gtttcatcag cactacattt actcatcgtt gatttcctct
     4861 gtatgtgcac ccaaggtgcc agataaacgt tgtggatatt ttacgcttcc ggaaagtgct
     4921 gctccagttg ttaattctgc aaaatcggat aagtgaccga aatcacactt aaaaatgatc
     4981 taaaacaaaa ttcacccgaa tccgcgagtg cgcaacctcc aaattttgcc agctggatcg
     5041 cgtttcttag atcatatttg aaaaaagatg gaaacatact tgcaacattc cagctggtcc
     5101 gacctatact ctcgccactg gtctgatttc taagatgtac ctcagaccct acacttcgcg
     5161 ctcctgttac agcacgtaac atagtttgta taaaaataaa tcattgaggt tatggtcatg
     5221 agccagaaaa ccctgtttac aaagtctgct ctcgcagtcg cagtggcact tatctccacc
     5281 caggcctggt cggcaggctt tcagttaaac gaattttctt cctctggcct gggccgggct
     5341 tattcagggg aaggcgcaat tgccgatgat gcaggtaacg ttagccgtaa ccccgcattg
     5401 attaccatgt ttgaccgccc gacattttct gcgggtgcgg tttatattga cccggatgtg
     5461 aatatcagcg gaacgtctcc gtctggtcgt agcctgaaag ccgataacat cgcgcctacg
     5521 gcatgggttc cgaacatgca ctttgttgca ccgattaacg accaatttgg ttggggcgct
     5581 tctattacct ctaactatgg tctggcaaca gagtttaatg atacttatgc aggcggctct
     5641 gtcgggggta caactgacct tgaaaccatg aacctgaact taagcggtgc gtatcgctta
     5701 aataatgcat ggagctttgg tcttggtttc aacgccgtct acgctcgcgc gaaaattgaa
     5761 cgtttcgcag gcgatctggg gcagttggtt gctggtcaga ttatgcaatc tcctgccggg
     5821 caaactcctc aagggcaagc attggcagct accgccaacg gtatcgacag taataccaaa
     5881 atcgctcatc tgaacggtaa ccagtggggc tttggctgga acgccggaat cctgtatgaa
     5941 ctggataaaa ataaccgcta tgcactgacc taccgttctg aagtgaaaat tgacttcaaa
     6001 ggtaactaca gcagcgatct taatcgtgcg tttaataact acggtttgcc aattcctacc
     6061 gcgacaggtg gcgcaacgca atcgggttat ctgacgctga acctgcctga aatgtgggaa
     6121 gtgtcaggtt ataaccgtgt tgatccacag tgggcgattc actatagcct ggcttacacc
     6181 agctggagtc agttccagca gctgaaagcg acctcaacca gtggcaacac gctgttccag
     6241 aaacatgaag gctttaaaga tgcttaccgc atcgcgttgg gtaccactta ttactacgat
     6301 gataactgga ccttccgtac cggtatcgcc tttgatgaca gcccagttcc ggctcagaat
     6361 cgttctatct ccattccgga ccaggaccgt ttctggctga gtgcaggtac gacttacgca
     6421 tttaataaag atgcttcagt cgacgttggt gtttcttata tgcacggtca gagcgtgaaa
     6481 attaacgaag gcccatacca gttcgagtct gaaggtaaag cctggctgtt cggtactaac
     6541 tttaactacg cgttctgata acgcgttcgc ctggataaag tcacctgcat agcaggtgac
     6601 tttaactccc ccacttcacc gaaagtagtg tcctcattgc ttacctcctg agttttgcaa
     6661 acagcctgtt ggcaggcttg ccctttcaaa tcaataagcg gtatccacag acaaacctga
     6721 agggaaaggc atattttcag gcgttctgct cgtccttctc aaagagttta cttttctgca
     6781 tttccaggat ac
//
LOCUS       DAZPEB010000196         6761 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZPEB010000196 DAZPEB010000000
VERSION     DAZPEB010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6761
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.201"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            52..936
                     /locus_tag="ACHURJ_003572"
     CDS             52..936
                     /locus_tag="ACHURJ_003572"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFR1282364.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFCPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSISMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(987..1841)
                     /gene="yeaE"
                     /locus_tag="ACHURJ_003573"
     CDS             complement(987..1841)
                     /gene="yeaE"
                     /locus_tag="ACHURJ_003573"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFR1282365.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGRQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISSAQVLLARVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(1931..2677)
                     /gene="mipA"
                     /locus_tag="ACHURJ_003574"
     CDS             complement(1931..2677)
                     /gene="mipA"
                     /locus_tag="ACHURJ_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFR1282366.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            3113..5047
                     /gene="yeaG"
                     /locus_tag="ACHURJ_003575"
     CDS             3113..5047
                     /gene="yeaG"
                     /locus_tag="ACHURJ_003575"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFR1282367.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            5160..6443
                     /locus_tag="ACHURJ_003576"
     CDS             5160..6443
                     /locus_tag="ACHURJ_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFR1282368.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAMNKRSVT
                     DVDSGESISIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            6590..>6761
                     /locus_tag="ACHURJ_003577"
     CDS             6590..>6761
                     /locus_tag="ACHURJ_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFR1282369.1"
                     /translation="MIQSTRISMGLFFKYFLSLTKIDPGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQS"
BASE COUNT         1779 a         1671 c         1626 g         1685 t
ORIGIN      
        1 cttcggtcgc tctttttttt acctgataaa atgaagttaa aggactgcgt catgattaag
       61 aaaatttttg cccttccggt catcgaacaa atctcccctg tcctctcccg tcgtaaactg
      121 gatgaactgg acctcattgt ggtcgatcat ccccaggtaa aagcctcttt tgcattacag
      181 ggcgcacacc ttctctcgtg gaaacctgcg ggtgaagaag aagttctgtg gttgagcaac
      241 aacacaccgt tcaaaaatgg cgtcgctatt cgcggtggcg taccggtttg ctggccgtgg
      301 tttggtccgg cggcacaaca aggtctgcct gcgcacggtt ttgcccgcaa cctgccgtgg
      361 acgctgaaat cacatcatga agatgctgat ggcgtagcgc tgacttttga attgacgcaa
      421 agcgaagaga cgaaaaaatt ctgtccgcac gactttacgc tgttagcgca tttccgcgtg
      481 ggtaaaacct gtgaaatcga tcttgaatca catggcgaat ttgaaaccac ctctgccctg
      541 catacctact ttaacgtggg tgatatcgct aaggtaagcg tcagtgggct gggcgatcgc
      601 ttcattgata aagtgaatga cgcgaaagaa gatgtactga ccgacggtat tcagaccttc
      661 cctgaccgta ccgatcgcgt gtatctgaat ccgcaagatt gcagcgtgat taatgatgaa
      721 gcgctgaatc gtattatcgc cgtaggccac cagcatcatc tgaacgttgt aggctggaac
      781 ccgggaccgg cgctttcaat tagcatgggc gatatgccgg atgatggcta caaaacattt
      841 gtttgtgtag aaacggctta cgcttcagaa acgcaaaaag tcaccaaaga gaaacctgca
      901 catctggcgc aatccattcg cgttgcgaaa cgttaatttg cgttaatgtt gtgtgccggg
      961 tgcgatgcat ccggcacgta tcaacatcac accatatcca gcgcagtttt tccttttggt
     1021 gccggatacg ctttatccag catggttaat tccgctgacg aaagttcgac ctcaagcaca
     1081 gccgcattct gttgaacatg ggcaatcgtg gccgcttttg gaatcgccat cacaccctga
     1141 tggctgatta ccctcgccaa caatacttgt gccgagctga tattgtgagc atgtgcaatt
     1201 tcgttgacta ccgcgttttt taacagtcca ttgcgcaacc gcccggcctg ggctaatgga
     1261 ctgtaagcca tcaccggcat ctgctgttgc tggcaccagg ggagtagatc gtactcaatc
     1321 cctcgtgaac cgagatggta aagcacctga ttagtggcac actgatttcc ccccggcagc
     1381 tgccagagtt cctgcatatc agcataatca aggttagaaa cgccccagcg gcggattttt
     1441 ccctgggcga tcaatttttc catcgctgca acagtctctt caaaagcgaa actgccagac
     1501 cagtgtaata agtagagatc gagataatca gtattgagac ggcgtaaact ggcttcgcat
     1561 gcatttatcg ctttttgcct gccagcattc cacggataga ctttagagac gagaaagacg
     1621 ttatcacgca gaccggttaa tgcttcccca accacctttt cggcaccgcc atcggcatac
     1681 atttcggcgg tatcaatgag ggttaaaccg agctcaatgc ccgcgcgtag tgcagcaact
     1741 tctgttttgc gctgactggc atcttcgccc atataccatg ttccctgccc tatggctggc
     1801 agtgagacat cgccactaaa ttgaatcatt ttttgttgca ttgtttcctc ccaggtattg
     1861 caccaccgta atgcaaaaca gggcgtgacg ccctgttttt atgcacaaaa tgccctggaa
     1921 agatgcatta tcagaatttg taggtgatcc cggtagaaat caggccagtc caggatttat
     1981 ccaccatcgg gctgtcagta acttcatcag acagacgggt atagcgcgcg gtaccgtaaa
     2041 cactccagtc gccgaggaag ttgtagctgg cgctcagctc caggtaaggg ctccagctgt
     2101 cgttcgggtt atagccacgc agaccgctgc gagcggactc tttgcgcgat acgccataat
     2161 agtattcgtt ctggttttcg ctgttccact gcacaccaat acccggagtc acggtcaggc
     2221 caccgttggt gtaacgatac aaccaggcca tatcccagac aatgccgttg ctgttatcca
     2281 gggtatcgcc agccagggtg gtacgcaggt aaccgtactg ggtaaagtga gcataagaca
     2341 gaccagccat catggtgctc ttacggtcat ccaggtgacg catttggtga tcgccactgt
     2401 ctttcgcttt gaagtaaagc ggcgaccagt aagcggtaat tgaaagttta tccgttgcgt
     2461 cattccacag gtagtaacca ccacctaagc cacggaacca gaagttatcg ccttcatagt
     2521 tgattaccgg tactgggtaa acatcggtat cgtaatcttt atatgggtgc tcaacgacac
     2581 ctacgcctgc gcccaaggaa aatttacctt cagcgtgcgc tacgcctgca gacgttgcga
     2641 taagcactcc aagtgccaga agtttgagtt tggtcacaat taatcattcc ttaaacaaat
     2701 gtttagcggg cgacaaagtt tacccgtcaa tacatcgaaa cccaaccttt ttacgttttc
     2761 attttttaaa gtaactgttt aattttcctg ccgcggatga caccgcgctt acagccaaat
     2821 gaatttagcg ttactggcga gcctggtctt tacattaatt atgcaaaatt tatggatgag
     2881 ttgttgatat gccattgaaa ttaagaaagc cgtgcaggca agttttccat ttgccatcta
     2941 cgcttaattt tgaaggtgta tcaccgggca cgttgttctc atcgtcgata aaatggcatg
     3001 agagttgctg tgttttagca agagacgtcg ttcagtttac ctcttccggg aggctctact
     3061 attcatatga acggctctta acctgtgcta aaaaacgaaa ggacggcata ccatgaatat
     3121 attcgatcac tatcgccagc gatatgaagc tgccaaggac gaagagttca cactgcagga
     3181 gtttcttacc acttgtcggc aagatcgcag tgcttatgcc aacgcggctg agcggctatt
     3241 gatggctatc ggtgagcctg tcatggtcga tacagcccag gaacccagac tttctcgact
     3301 cttttctaac cgggtcattg cacgttatcc ggcgtttgaa gagttttacg gcatggaaga
     3361 cgcgattgaa cagattgtct cttatctgaa acacgcggct caggggctgg aagagaagaa
     3421 acaaatcctg tatctgctgg ggcctgtggg tgggggtaaa tcatcgcttg ctgagcgact
     3481 gaaatcatta atgcagctcg taccgattta tgtattgagc gccaacggtg agcgtagccc
     3541 ggtcaacgat catccgttct gtcttttcaa tccgcaggaa gatgcgcaga ttctggaaaa
     3601 agagtatggc attcctcgcc gttatctcgg caccatcatg tcgccgtggg cggcaaaacg
     3661 cctgcatgaa tttggtggcg atatcactaa gttccgggta gtgaaggtct ggccgtcaat
     3721 tctgcaacaa attgctatcg ccaaaacgga acccggcgat gagaacaacc aggacatctc
     3781 cgcgctggtt gggaaagtcg atattcgtaa actcgaacac tacgcgcaga atgacccgga
     3841 cgcctacggc tattccggtg cgctgtgccg cgccaatcag gggatcatgg aattcgttga
     3901 gatgtttaaa gcaccgataa aagtgctgca tcccttgtta accgccactc aggaaggtaa
     3961 ctacaacggg acggaaggta tctccgccct gccgttcaac gggattattc tcgcccactc
     4021 gaacgagtcc gaatgggtca ctttccgtaa taacaaaaac aacgaagcct tcctcgaccg
     4081 tgtttacatc gtgaaggtgc cgtattgctt gcgcatttcc gaagagatca aaatctacga
     4141 gaaattgctt aatcacagtg aattgactca cgccccatgc gcccctggca cgctggaaac
     4201 actgtcacgt ttttccattc tttcgcgcct gaaagagcca gaaaactcca gcatttattc
     4261 aaagatgcgg gtttatgatg gcgaaagtct gaaagacacc gatcccaaag ccaagtcgta
     4321 tcaggaatat cgtgactacg ccggtgtcga tgaagggatg aacggtctgt cgacgcgttt
     4381 tgcgtttaag atcctctccc gcgtgttcaa cttcgatcat gtagaagtgg cggcaaaccc
     4441 ggtccatctg ttctacgtcc tggaacagca gatcgagcgc gagcagttcc cgcaagagca
     4501 ggcagaacgc tatctggagt tcctgaaagg ttatctgatc ccgaaatatg ccgagtttat
     4561 cggcaaagag atccagacgg cctaccttga atcctattcc gaatatgggc aaaacatttt
     4621 cgaccgttat gttacctacg cggatttctg gattcaggat caggagtatc gcgatccgga
     4681 taccgggcag ctgtttgacc gcgagtctct taacgccgag ctggagaaaa tcgagaaacc
     4741 ggcggggatc agtaatccaa aagatttccg caacgagatt gttaacttcg tactgcgcgc
     4801 cagagcgaat aacagcggac gcaatccgaa ctggaccagc tatgaaaaac tgcgcacggt
     4861 tatcgagaag aaaatgttct ccaataccga ggagctgttg ccggttatct cgtttaacgc
     4921 caaaacgtca accgacgagc agaagaaaca cgacgacttt gtcgaccgta tgatggaaaa
     4981 aggctacacc cgtaaacagg tgcgtttact gtgcgaatgg tatttgcgcg tacgtaaatc
     5041 gtcttaacaa ccctggcccg gtacacatgc taccgggcct acaacgacag cgaaccgtgg
     5101 gccagagaaa tggcaacaca ggcgtagcat acagttggca aatgtagtac ggggggcata
     5161 tgacctggtt tattgaccgg cgtctgaacg gcaaaaacaa aagcatggtg aatcgccagc
     5221 gttttttacg ccgttataaa gcgcaaatta aacagtcgat ctccgaggcc atgaataagc
     5281 gttcggtgac tgacgtcgac agcggcgaat ccatatccat tcccacggaa gatattagcg
     5341 aaccgatgtt tcatcagggg cgtggcggcc tgcgccaccg cgtacatccg ggcaatgacc
     5401 atttcgtcca gaacgaccga attgaacgtc cccagggtgg cggcggaggt tccggcagtg
     5461 gtcagggcca ggccagccag gatggtgaag gtcaggatga atttgtcttt cagatttcga
     5521 aagatgagta tcttgatctg ctctttgaag atttggcctt accgaatctg aaacaaaacc
     5581 aacaacgcca gctgaccgaa tataaaacgc atcgggcggg ttataccgct aacggcgttc
     5641 cagccaatat cagcgttgtg cgttccttgc agaactcact ggcgcgacgc acagccatga
     5701 cggcgggcaa gcgacgggaa cttcatgcgc tggaagagaa tttggccatc atcagcaaca
     5761 gtgaacctgc gcaactgctg gaagaggaac gtctgcgcaa agaaattgca gaattacgtg
     5821 ccaaaattga acgcgtccct tttattgaca ccttcgattt acgttacaag aactacgaga
     5881 agcggcccga tccctccagc caagcagtga tgttttgcct gatggacgtt tccggttcaa
     5941 tggatcaatc cactaaagat atggctaagc gtttttatat tctgctgtat ctgttcctca
     6001 gcagaacgta taagaacgtg gaagtcgtat acatccgcca tcatacccaa gcgaaagaag
     6061 tcgatgaaca tgagtttttc tactcgcagg aaacaggcgg caccattgtt tccagcgccc
     6121 tgaaactgat ggatgaggta gtgaaagagc gttataaccc ggcacagtgg aatatttacg
     6181 ctgcacaagc atcggacggc gataactggg ccgatgactc tccgctttgc catgaaatcc
     6241 tggcgaaaaa attattacct gttgttcgtt attacagcta tatcgaaatt acccgtcgtg
     6301 cacatcagac attgtggcga gaatatgagc atctgcaatc tactttcgac aactttgcga
     6361 tgcagcacat ccgcgaccag gatgatattt atccggtgtt ccgtgaactg tttcataaac
     6421 aaaatgcaac agctaaagac taaaactatc agccaggtca ttatcgcctg gctgattttt
     6481 agcttactgt aaattatctc atttattaca tacattagct tacaatcgct ttaaatatga
     6541 cagcataacc tttacataat ttagttccag aaaacaatca ttcggaaaaa tgattcagtc
     6601 aacacgtatt tccatggggt tattctttaa atatttttta tcgttaacga aaattgatcc
     6661 tggtcaaaac tatatatctc tgccatcaat aaaatccagc actcacattg ctctcctttt
     6721 tatggtttct atgggtacac aaaaattaaa agctcaaagc t
//
LOCUS       DAZPEB010000197         6627 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZPEB010000197 DAZPEB010000000
VERSION     DAZPEB010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6627
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.202"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            566..3979
                     /locus_tag="ACHURJ_003578"
     CDS             566..3979
                     /locus_tag="ACHURJ_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFR1282370.1"
                     /translation="MEQSLNFEMLRSQWPELAELACMAERYVHSDPESCLVKLRNYTE
                     LMVRWLYRQERLPEGIKANLYDLMNADVFTSMMPEAIIMKMDALRIHGNRAAHGGRIK
                     AKDTYWLLKEAYLLGIWLYVRYAHGNVDDCPKFTLPPLTQSSGRADEKRLEDAIKAQD
                     ESRERELALQRALQQEQEKAEHLTQRLNEARARNQHVADILSIDEAETRRRLIDSRLL
                     AADWNVGEELKNTDQVTQEHPVKEQPTTSGDGYADYVLWDEAHKPLAVVEAKKTSVNA
                     EQGRIQARLYADWLEKEYGQRPVIFYTNGYDIWLWDDHKTHGYPPRRVFGFYSKESLQ
                     YLIQQRETRLPLNRVPHVKDNEGKAVAGRLYQLETIARVSERFTNKYRQSLIVQATGT
                     GKTRVAIALSKLMIDARWVKRVLFLCDRKELRKQAANAFNQFTNEPLYVVGKSKKADR
                     QNARIYIATYPGMMKIMDHFDVGYFDLIIADESHRSIYNVYGDLFKYFDALQIGLTAT
                     PIDMVSKTTFGLFGCEGRIPTANYSLEDAIADNNLVPYEVVTHTTEFLREGIKREKLS
                     DAQIRELEEQGIDPNTLEFDGKALDEAIYNKDTNRYILRNLMENGLKDRDGQLPGKTI
                     IFARNHKHALLLNELFDDMYPQFAGRFCQVIDNYDPRAEQLIDDFKGLDESTNKELTI
                     AISVDMLDTGIDVPEIVNLVFAKPVKSKVKFWQMIGRGTRLCPGLYGYDDNGKPLDKQ
                     KFRIFDHWGNFEYHELHTEEAEVTATKSLAQKRFEAWVMLGAAAQRKFDKQAVDLVAH
                     QLREQINALDEKSIAVQEKWQQKAQYSDEKVLRQLSPKTQQDLLSVLAPLMQWLDVRG
                     QSDAIRFDMDILAAQTARYTNPEELDVLWPIIVEKVERLPPHLAQVQQQGQRINQLRN
                     LSWWKQASLEELEDIRIHLRGIMHLMEKDATPKFGSIQVDITEDANLIQTETRKTNIR
                     SIDFKLYRQQVQGALEPLFQQNPVLKKIRNGEPVTQNELDELAKLVLIQNPNVDIRAL
                     KEFYPQATASLDKLLRTIIGMDSDAVEVRFAQFAADNSLTSQQLRFLSLLKNHIRDYG
                     TIEMRQLFEQPFTHIHNEGVTGVFPDIAQIARLQKIVEELGVVTDAATV"
     gene            4043..5677
                     /locus_tag="ACHURJ_003579"
     CDS             4043..5677
                     /locus_tag="ACHURJ_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFR1282371.1"
                     /translation="MITGDLKSKIDGLWEDFWVGGITNPLTVIEQITYLMYSRMLDTQ
                     EQRDEKRKQIAGIDFKPRFAPEQQEFRFSHYSNLGSDEMMEVVRDGVFQHFRQLGQAD
                     ASKVTLLGNFMKDARLEIVKPSLLTKAVEVIKNLPLDRGDTKGDLYEYLLSKLTTAGI
                     NGQFRTPRHIIRTMVEMMEPNPARGETICDPACGTGGFLATSYEYLLEKYSSLESIHT
                     EIGTNERGELEEQKIFTGDLLTPWRNHVDNNMFHGYDFDTTMLRIAAMNLIMHGVDAP
                     DIHYQDTMSQSFSTNFPQASKNAFNLILANPPFTGSLDEEDIDSTLSAMVKTKKTELL
                     FLARILQMLKVGGRSATIVPQGVLFGSSKAHQSLRKTLVEDNQLETVINLPSGVFKPY
                     AGVATAILIFTKGGQTDEVWFYDLQNDGYSLDDKRNPIKDNDLPHLLASWKHYRTLRG
                     LPVDNFMGKKLASLLKQQYPEGINAGVDFKDRTQAAFVVPKADIAAQKYDLSINRYKE
                     VVYQAEEYEDPKVILKRLKDLEKEILADLDELEGML"
     gene            5686..>6627
                     /locus_tag="ACHURJ_003580"
     CDS             5686..>6627
                     /locus_tag="ACHURJ_003580"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001438891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFR1282372.1"
                     /translation="MVKLGDVINVHYGKALKADQRVSNGSVHVFGSSGIVGNHDKTLC
                     SYPTIIIGRKGSVGAITWAPSGGWIIDTAYYVEIKDNNKLDLRYLFYILSGIDLTKKT
                     ITTSIPGLNRDDLYDTFIKLPPFEEQKRIVDLLDKAEGIRQKREQSIKLADDFLRATF
                     ATMYGNPITNPKKWPVHLMGEIIEFKGGNQPPKSDFIFEPKQGYIRLVQIRDFKSDKY
                     ATYIPQEKAKRIFEVDDVMIARYGPPVFQILRGLSGSYNVALMKASPKENIRKGFIFY
                     LLQLPEYHDVVVKNSERTAGQTGVNLELLNKFNVPLPP"
BASE COUNT         1879 a         1538 c         1661 g         1549 t
ORIGIN      
        1 gtcctgttct cccaaatatc atggtattga tacgggagat tatgccgtac aggtacgaca
       61 cgttatgtcg tgccaaaggg gagtggatag aaaaaatggc gaagatgcga aaagttgatt
      121 aatgcattga aaaaaaatga attaataatg aacttgggta aaagcgccat caaaatacga
      181 cacgacctgt cgtattttgt gttagccggg aaaagatatc tgctacttac taaaatgatt
      241 tagtgctgaa aatatttata agccaataaa tagacaggta ttacggagta acgcccaggc
      301 tattttaacg agcctgctgt tttattggct caaaaaacat atcccataga tgatcagcca
      361 tacccgcgtt agtgagcaag gtatcccttg agtagccagg gctgaagacg cccattgatg
      421 ggcgaaaacc aaggttctgg ccgttaaagc ggctatgtcg ttaaacacat caccaccata
      481 tgccatccgg ctcacttcgt cggccaaatt agtgtgatat tctttccaga atttctgttg
      541 tatccacaac ataaggcgat gattgatgga gcagtctctc aattttgaaa tgttgcgcag
      601 ccagtggccg gaactggcag aactcgcatg tatggcagag cgctatgttc actccgatcc
      661 ggaaagctgc ctggttaagc tgcgcaacta caccgaattg atggtgcgct ggttgtatcg
      721 tcaggagcgg ttgccggaag gtattaaggc taatctttac gatttaatga acgctgatgt
      781 ctttaccagc atgatgccgg aagccatcat catgaaaatg gatgcgctgc gtatccatgg
      841 caaccgtgcc gcgcacggcg gacgtatcaa agctaaagat acttactggc tgctcaaaga
      901 agcgtatttg ttgggaattt ggctgtatgt tcgctacgcc cacggtaatg ttgatgactg
      961 ccccaaattt acactccctc cattaacaca atcttcaggt cgtgcagatg aaaaacgtct
     1021 ggaagatgca atcaaggctc aggatgaaag ccgtgagcgc gaactggcgc tacaacgcgc
     1081 actacagcaa gaacaggaaa aggccgaaca cctcactcaa cgcctgaatg aagccagagc
     1141 gcgtaaccag catgttgccg atattctctc tattgatgaa gcggaaaccc gccgtcgcct
     1201 gattgactct cgcttacttg cagccgactg gaatgtaggg gaagaactta aaaacacgga
     1261 tcaagtcaca caagaacatc cagtcaaaga gcaacccacc acctctggtg acggttatgc
     1321 agactatgtc ttgtgggatg aggcacacaa accgttggcg gtggtggaag caaaaaaaac
     1381 cagcgtcaat gccgagcaag gacgaattca ggcccggctg tatgcagact ggctggaaaa
     1441 agaatacggt cagcgtccgg tcatcttcta caccaacggc tatgatatct ggctgtggga
     1501 tgaccataaa actcatggtt atcccccgcg tcgggtgttt ggcttctaca gcaaggaaag
     1561 cctgcaatat cttattcagc agcgtgaaac ccgtctgccg ctgaacagag tgccacacgt
     1621 aaaagataac gaaggtaagg ccgttgccgg acgtttgtac cagctcgaaa ccattgcccg
     1681 cgtcagtgaa cggtttacca acaagtaccg acaatcgtta attgttcagg ctacgggtac
     1741 aggcaaaacc cgcgtggcaa ttgcactaag caaactgatg attgatgccc gctgggtaaa
     1801 acgcgtcctg tttctttgcg accgtaaaga actgcgtaaa caggcggcaa atgcctttaa
     1861 tcaattcacc aatgagccgc tgtatgtggt cgggaaatcg aaaaaagcag acaggcagaa
     1921 tgccagaatc tacatcgcta cctatcccgg tatgatgaaa atcatggacc atttcgatgt
     1981 cggttatttc gatttgatca tcgccgatga atcccaccgc tctatctaca acgtctacgg
     2041 cgatctgttt aagtattttg atgccctcca gattggcctg acggccacgc caattgacat
     2101 ggtgagtaaa accactttcg gcctgtttgg ttgtgaagga cgtattccta ccgccaacta
     2161 cagcctggaa gatgccatcg ccgataacaa tctggttccc tatgaggttg tcacacacac
     2221 aacagaattc ctgcgcgaag gtatcaaacg ggagaaatta agcgacgcac aaatccgcga
     2281 gctggaagaa caaggcatag atcccaatac gctggagttt gatggcaagg cgctggatga
     2341 ggcgatctac aacaaagaca ccaaccgcta tatcctgcgt aacctgatgg aaaatggcct
     2401 gaaagatcgg gacggccagt tgcctggtaa aaccatcatt ttcgcccgta accacaaaca
     2461 cgcgctgctg ttgaatgaat tgttcgacga tatgtaccca cagtttgccg ggcgcttctg
     2521 ccaggtcatc gacaactacg atccccgcgc cgagcaactg attgatgatt ttaaagggct
     2581 ggatgaaagc accaacaaag agctgaccat cgctatctcc gttgacatgc tcgataccgg
     2641 tattgatgtc ccggaaattg tgaatctggt ctttgccaaa ccggtcaaat caaaagtgaa
     2701 gttctggcag atgattggcc gtggtacgcg cctctgtccg gggttgtacg gttacgacga
     2761 taacggcaaa ccgctggata aacagaaatt ccgcattttc gatcactggg gcaattttga
     2821 gtatcacgag ctgcataccg aagaggcgga agtcacagcg acaaaatcgc ttgcgcaaaa
     2881 acgcttcgaa gcgtgggtta tgctgggggc cgctgcgcag cgtaaattcg acaaacaggc
     2941 ggtggattta gttgctcacc agcttcgcga gcaaatcaac gcactggatg aaaagtcgat
     3001 tgccgtacag gaaaaatggc agcagaaagc gcagtacagt gatgaaaagg tgctgcgcca
     3061 gctttctccg aaaacacagc aggatctgct atctgttctg gccccgttga tgcagtggct
     3121 ggacgtgcgc gggcaaagcg atgccatacg ctttgatatg gatattctgg cggcgcaaac
     3181 tgcccgttat accaacccgg aagagctgga tgttctctgg ccgatcatcg tcgaaaaagt
     3241 ggagcgcctg ccgccgcatt tggcgcaggt gcagcaacag ggacaacgaa tcaatcagct
     3301 tcgtaattta agctggtgga agcaggccag cctggaagag ctggaggata ttcgcattca
     3361 tttgcgcggt atcatgcacc tgatggaaaa agacgcgacg cctaaatttg gttcgataca
     3421 ggtggatatt accgaagatg cgaacctgat ccagacggag acccgcaaaa ctaatatccg
     3481 ctcgattgat ttcaaactct atcgccagca ggttcagggg gcgctggagc cgctgttcca
     3541 gcaaaacccg gttctgaaga aaatccgcaa cggcgagcca gtcacgcaaa acgagctgga
     3601 tgagctggcg aagctggtgc tgatccagaa ccccaacgtt gatattcgtg cgttgaaaga
     3661 gttttatccg caggcaaccg ccagcctgga taaacttttg cgtaccatca tcgggatgga
     3721 cagcgacgcg gtggaagtgc gttttgccca gttcgccgct gataacagcc tgaccagcca
     3781 acaactgcgc ttcctgtcat tgctgaaaaa ccacattcgc gattacggca ccattgaaat
     3841 gcggcagctc tttgaacagc cttttacgca tatccacaac gaaggcgtta ccggtgtgtt
     3901 ccctgatata gcgcaaatcg cccgcctgca aaagatagtc gaagagctgg gtgttgtgac
     3961 cgacgcagcg acggtataat acacgcatga caagccccgg cagcggggct tttgtattta
     4021 aaacaggata taaacgctaa caatgattac tggcgactta aaaagtaaaa tcgacggact
     4081 atgggaagat ttctgggtgg gtggtatcac caacccgctg accgtaatcg aacagatcac
     4141 ctatctgatg tattcccgga tgctggatac ccaggaacaa cgcgacgaaa agcgtaaaca
     4201 aatcgcgggt attgatttta aaccgcgttt tgcgccagaa cagcaggagt tccgtttcag
     4261 tcactatagc aaccttggct cggatgagat gatggaagtg gtgcgcgatg gcgtattcca
     4321 gcatttccgt cagctcggcc aggccgatgc ttcgaaggtg acgctgctgg gcaactttat
     4381 gaaagatgct cgtctggaga ttgttaagcc gtcattgctg accaaagcgg ttgaggtaat
     4441 caaaaacctg ccattggatc gcggcgacac caaaggcgac ctttacgaat acctgttaag
     4501 caagctgaca actgccggaa tcaacggaca gttccgcaca ccgcgccaca ttatccgcac
     4561 gatggttgaa atgatggagc cgaacccggc ccgcggcgag acgatttgcg atcccgcctg
     4621 tggcaccggt ggttttctgg caaccagcta tgaatatctg ctggagaagt acagctcgct
     4681 ggaatccatt catactgaga ttggcaccaa cgaacgtggc gagctggaag agcaaaaaat
     4741 ctttaccggc gatctgctga caccgtggcg taaccatgtg gataacaaca tgttccacgg
     4801 ttacgacttt gacaccacaa tgctacgtat cgccgccatg aacctgatta tgcacggcgt
     4861 ggatgcgcct gatatccact atcaggacac aatgagccag agtttcagca caaacttccc
     4921 gcaggccagt aaaaacgcct tcaacctgat tctggcgaac ccgccgttta ccggttcact
     4981 ggacgaggaa gatatcgact ccacgctgtc ggcaatggtg aaaaccaaaa aaaccgaact
     5041 actgttcctg gcgcgtattc tgcaaatgct gaaagtgggc gggcgcagtg ccactatcgt
     5101 gccgcagggc gtgctgtttg gctctagcaa ggcgcaccag tcacttcgca aaacgctggt
     5161 ggaagataac caactggaaa cggtgatcaa tctgccttct ggtgtattta aaccttacgc
     5221 tggcgtggcg acggcgatcc tgatctttac caaaggcggt caaacggatg aggtctggtt
     5281 ctacgatcta caaaatgacg gctacagcct ggatgataag cgcaacccga taaaagacaa
     5341 cgatctgccg catctgctgg caagctggaa gcattaccgt actttacgcg ggctaccggt
     5401 tgataacttt atgggtaaga agttagcctc gttgcttaaa cagcagtacc cggaagggat
     5461 taatgctggc gttgatttta aagatcgcac gcaggcggcg tttgttgtac cgaaagcgga
     5521 tattgctgcg cagaaatacg atctatccat caaccgttat aaagaagtcg tgtatcaggc
     5581 ggaggaatat gaagatccga aggtgatatt gaagcggtta aaggatctgg aaaaagagat
     5641 tctggcggat ttggatgagc tggaggggat gctgtgagtt cggcgatggt gaagttaggg
     5701 gatgttatta atgttcacta cggaaaagca ttaaaagctg atcagcgtgt atcgaatgga
     5761 agtgtccatg tgtttggtag ttcaggaatt gtcggcaacc acgataaaac gctttgcagc
     5821 tatcccacga taattattgg ccgcaaaggc agcgttggtg ctattacctg ggctccaagc
     5881 ggaggctgga tcattgatac tgcttattac gttgagatca aagataataa taagttagat
     5941 ctccgatact tgttttatat tctgagcgga attgatctca ctaagaaaac gattaccaca
     6001 agtattcctg gattaaatag agacgatctc tatgatacat ttattaaatt accacctttt
     6061 gaagagcaga aacgaattgt ggatcttctc gataaagctg agggtatacg ccaaaaacgt
     6121 gagcaatcca tcaaactggc tgatgatttt ttgcgtgcta cgtttgctac aatgtatggg
     6181 aatcctatta caaaccccaa aaaatggcct gtccatctta tgggagaaat tattgaattt
     6241 aaaggtggaa atcaaccacc taaaagtgat tttatttttg aacctaaaca gggttatatt
     6301 agattagtac aaattagaga ttttaaatcc gataaatatg cgacgtacat tccgcaagaa
     6361 aaagcaaaac gtattttcga agttgatgat gttatgattg ctcgttatgg gccacctgtg
     6421 tttcaaatac ttagaggttt atccggcagc tacaatgtag ctttaatgaa agcttcaccc
     6481 aaagaaaata ttaggaaggg gtttatattc tatcttctac aacttccgga atatcatgat
     6541 gttgttgtaa aaaactctga aagaacagca ggacaaaccg gtgtaaatct tgaactatta
     6601 aacaaattca atgttcccct cccacct
//
LOCUS       DAZPEB010000198         6591 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZPEB010000198 DAZPEB010000000
VERSION     DAZPEB010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6591
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.203"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            18..173
                     /locus_tag="ACHURJ_003581"
                     /pseudo
     CDS             18..173
                     /locus_tag="ACHURJ_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            166..1947
                     /locus_tag="ACHURJ_003582"
     CDS             166..1947
                     /locus_tag="ACHURJ_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFR1282373.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKMVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            2128..2466
                     /gene="glnK"
                     /locus_tag="ACHURJ_003583"
     CDS             2128..2466
                     /gene="glnK"
                     /locus_tag="ACHURJ_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFR1282374.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            2496..3782
                     /gene="amtB"
                     /locus_tag="ACHURJ_003584"
     CDS             2496..3782
                     /gene="amtB"
                     /locus_tag="ACHURJ_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFR1282375.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLMQVTVTFALVCILWVVYGYSLAFGEGNNFFGNVNW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQELEGLDVNSHGENAYNA"
     gene            complement(3831..4691)
                     /gene="tesB"
                     /locus_tag="ACHURJ_003585"
     CDS             complement(3831..4691)
                     /gene="tesB"
                     /locus_tag="ACHURJ_003585"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFR1282376.1"
                     /translation="MSQALKNLLALLNLEKIEAGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            4909..5481
                     /locus_tag="ACHURJ_003586"
     CDS             4909..5481
                     /locus_tag="ACHURJ_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFR1282377.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(5512..5823)
                     /locus_tag="ACHURJ_003587"
     CDS             complement(5512..5823)
                     /locus_tag="ACHURJ_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFR1282378.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            5986..6082
                     /gene="ffs"
                     /locus_tag="ACHURJ_003588"
     ncRNA           5986..6082
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHURJ_003588"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            6202..6555
                     /locus_tag="ACHURJ_003589"
     CDS             6202..6555
                     /locus_tag="ACHURJ_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFR1282379.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
BASE COUNT         1445 a         1636 c         1916 g         1594 t
ORIGIN      
        1 gggatgcgtt catataaatt attgtgatgc agcacggaca tatcgcccag cgtggcaatc
       61 atgatgagtt ggcgcaacaa agcggctggt atcgcgatat gtatcgctat caacaactgg
      121 aggcggcgct cgacgacgct ccggaaattc gcgaggaggc cgtcgatgcg tagttttagc
      181 caactgtggc cgactctcaa gcgtctgtta gcgtacggtt cgccgtggcg taaaccgctg
      241 gggattgcgg tcctgatgat gtgggttgcg gcagcggcag aagtcagtgg gccgctgctt
      301 atcagctatt ttatcgacaa tatggtagcg aaaaataacc tgccgttgaa aatggttgca
      361 gggctggctg cggcgtatgt tgggctacaa ctgtttgccg ccgggctaca ttacgcgcag
      421 tcgctgctgt ttaatcgggc ggcagtaggc gtagtgcaac agttgcgtac cgacgtgatg
      481 gatgccgcat tgcgccagcc attaagcgag tttgataccc aacccgtcgg gcaggtgatt
      541 tcccgcgtca ctaatgacac tgaagtgatc cgcgatctct acgttaccgt agtggcaact
      601 gtcctgcgta gcgccgcact ggtgggcgcg atgctggtgg cgatgttcag cctcgactgg
      661 cgaatggcgc tggtggcgat aatgattttc ccggtggtgc tggtggtaat ggtgatatac
      721 cagcgttaca gcacgccgat tgtccgtcgt gtgcgcgcct atctggcgga tatcaacgac
      781 ggctttaacg aaatcatcaa tggcatgagc gttatccagc agttccgtca gcaggcgcga
      841 tttggcgaac gtatggggga ggccagtcgt tcacactata tggccaggat gcaaaccctg
      901 cgcctcgacg gttttctgct gcgtccgctg ctgagtctgt tttcatcgct cattctttgt
      961 ggcttgttga tgctgtttgg cttctccgct agcggcacca ttgaagtagg cgtgctgtat
     1021 gcgtttatca gctatcttgg gcgacttaac gaacccttaa tcgaactgac cacgcaacag
     1081 gcgatgctgc aacaggctgt tgttgctggt gagcgcgtgt ttgaactgat ggacggaccg
     1141 cgccagcaat atggcaatga tgatcgcccg ttacagagtg gcaccatcga agtcgataac
     1201 gtgtcatttg cttatcgcga tgacaatctg gtgctaaaga acattaatct ctctgtgcct
     1261 tcgcgtaact ttgtggcact ggtcgggcat accggcagtg gcaaaagcac cctcgccagt
     1321 ttattgatgg gctattaccc gctaacggaa ggtgagattc gccttgatgg tcgtccatta
     1381 agttcgctaa gtcacagcgc gctgcgccag ggcgtggcaa tggtgcagca agatccggtg
     1441 gtgctggcgg ataccttcct cgccaacgtg acgctggggc gtgatatctc cgaagaacgc
     1501 gtctggcagg cgctggaaac cgtgcaactg gcggagctgg cgcgcagcat gagtgatggt
     1561 atttatacgc cgctgggcga gcaggggaat aatctctccg tcgggcaaaa gcaactgctg
     1621 gcactggcac gcgtgttggt ggagacgccg caaatcctga tccttgatga ggcaaccgcc
     1681 agcattgact ccggtactga acaggcgatt caacatgctc tggcggctgt gcgtgaacat
     1741 accacgctgg tggtgattgc tcaccgctta tcgaccattg ttgatgccga caccattctg
     1801 gtgcttcatc gtgggcaagc cgtggagcag ggcactcacc agcaactgct ggcggcccag
     1861 ggacgctact ggcagatgta tcaactgcaa cttgcgggcg aagagctggc agccagcgtg
     1921 cgtgaagaag aatcattgag cgcctgaata gcgcaatatt tcatcgttgg tgcaaaaatg
     1981 taacgcactg tgcactgtca tagtgcgttt tcattttcaa tcttcttaac ttcctgctct
     2041 ctttctcgtt tttcatttct ggcacaccgc ttgcaatacc ttcttcgtgt agcagaacca
     2101 ttaccgaatt ctgactggag gggatctatg aagctggtga ccgtgataat caaaccattc
     2161 aagctggaag acgttcgtga agcgttatct tccattggta ttcagggcct gaccgtcacc
     2221 gaagtgaaag gtttcgggcg tcagaaaggg catgccgagc tgtaccgggg ggcggaatac
     2281 agcgtcaact tcctgccaaa agtaaaaatt gatgtggcaa ttgctgatga ccaactcgat
     2341 gaagtgatcg atatcgtcag taaggcggct tacaccggaa aaattggcga cggcaaaatc
     2401 ttcgtcgctg aattgcaacg cgtcattcgt attcgtaccg gcgaagccga cgaagcggcg
     2461 ctgtaatctc tggcacacag caacaggaac gaaaaatgaa gatagcgacg ataaaaactg
     2521 ggcttgcttc actggcgatg ctgccgggac tggtaatggc tgcacctgcg gtggccgata
     2581 aagccgacaa tgcgtttatg atgatttgta ctgcgctggt gctgtttatg actattccgg
     2641 ggattgccct gttttacggt gggttgattc gcggcaaaaa cgtgctgtcg atgctgatgc
     2701 aggtgacggt gacgtttgca ctggtctgta ttctctgggt ggtttacggt tactcgctgg
     2761 cgtttggtga gggtaacaac ttcttcggca acgttaactg gttgatgctg aaaaacatcg
     2821 aactgacggc ggtgatgggc agcatttatc agtatatcca cgtagcgttt cagggatcgt
     2881 ttgcctgcat taccgtaggc ttgatagttg gggcgctggc ggaacgaatc cgcttctcag
     2941 ctgtgttgat tttcgtggtg gtatggctga cgctctctta cattccgatt gcgcatatgg
     3001 tgtggggcgg cggtttgctg gcttctcacg gtgcactgga tttcgcgggt ggcacagtgg
     3061 tgcacattaa cgccgcaatc gccggtctgg tgggcgcgta tctgataggc aaacgcgtgg
     3121 gcttcggtaa agaggcgttt aaaccgcaca acctgccgat ggtcttcacc gggactgcca
     3181 ttctctatat cggttggttt ggctttaacg ccgggtcagc gggcacggcg aatgaaatcg
     3241 cggcactggc atttgtgaat actgtggtcg caacggcggc ggcaattctt ggctggatct
     3301 tcggtgaatg ggcgctgcgt ggtaagcctt cactgctggg ggcgtgttct ggcgcgattg
     3361 ccggtctggt cggcgtgacg ccagcgtgcg gctacattgg ggttggcggc gcgttgatta
     3421 tcggcgtggt agctggtctg gcgggcttgt ggggcgttac catgctcaaa cgcttgctgc
     3481 gggtggatga tccctgcgat gtcttcggtg tgcacggcgt ttgtggcatt gtcggctgta
     3541 tcatgaccgg gatttttgcc gccagctcgc tgggcggcgt gggcttcgct gaaggtgtga
     3601 cgatgggtca tcagttgctg gtacagctgg aaagcatcgc cattacgatc gtctggtccg
     3661 gtgtggtggc atttattggc tacaaattgg cggatctgac ggttggtctg cgtgtaccgg
     3721 aagagcagga gctagaaggg ctggatgtca acagtcacgg cgagaatgcc tataacgcgt
     3781 aacaagcact gcaaaaaaca gccggacggt tttcacctcc ggctattttt ttaattgtga
     3841 ttacgcatca ccccttcctg aacggtcgag gcaaccagta cgccgtcctg ggtataaaac
     3901 tcaccgcgca caaagccacg tgcgctggac gccgaggtgc tctccacgct atacagcagc
     3961 cattcattca aattaaacgg gcgatggaac cacatggaat ggtcaatggt ggcaatctga
     4021 atccccggtt cgagaaaacc gatgccgtgc ggctgtagag ctaccggcag gaagttaaga
     4081 tcagaagcgt aaccgagcag atactgatga acgcgcaggt catccggcac gctaccattt
     4141 gcgcggatcc acacctgacg atgtggttct gcgacgtgac ctttcagtgg gttatgaaac
     4201 tccaccggac ggacttccag cggacgatcg cagatgaatt tatctttaag cactggcggc
     4261 agcaggtgcg ccagcgattg ggcgatttgc gtttccgaag gcaggccatc aggcgctggc
     4321 gcggacggca ttgttttttg atgttcgaaa cccgcttctg gtgcctggaa agaggccgtc
     4381 atataaaaaa tcggtttgcc gttttgaata gcagcaaccc ggcgggcgct gaagctgtta
     4441 ccatcacgca gcgtttcgac atcataaata atcggtttct tactatcgcc tgggcgaaga
     4501 aagtagctgt gaaacgaatg taccagccgc tcttccggga cggtctcttt tgcagcatac
     4561 aaggcctgac ccacgacctg gccgccaaac acctggcgta aacctaaatc ttcactctgg
     4621 ccgcgaaaga gtcctgcctc aattttttcc agatttaaca atgccagtaa attttttagc
     4681 gcctgactca tataactctc cagtaacaaa gctgccgcag caagccaaag tgagttgagt
     4741 ataacgcaaa tttgccactg gtccgatggg tgcaataatc tgaattacgg gctaattaca
     4801 ggcagaaatg cgtgatgtgt gccacactta ttgacgttac tattttgtta accactcttc
     4861 cggcgaggaa agttagcccg ctggtgcatt gataataagg agaaatgaat gaaactcgtg
     4921 cacatggcca gtggtttagc ggttgcgatt gcgttggcgg cttgcgcaga taaaagcgcg
     4981 gatattcaga cgccagcccc ggctgcaaat acgtctattt cagcaacaca acaacctgct
     5041 atccagcaac cgaatgtctc cggtaccgtc tggatccggc agaaagtcgc actgccgcct
     5101 gatgctgtgc tgaccgtgac actttctgac gcgtcgttag ccgatgcacc gtcaaaagtg
     5161 ctggcgcaga aagcggtgcg taccgaaggt aaacagtcac cattcagctt tgttctgcca
     5221 tttaacccgg cagatgttca gccgaacgcg cgtattctgt tgagtgcggc gattaccgtg
     5281 aatgacaaac tggtatttat taccgatacc gttcagccgg tgatcaacaa gggcggaact
     5341 aaagccgacc tgacattggt gccggtgcag caaaccgccg tgccggttca ggccagcggt
     5401 ggtgcaacga ctaccgtacc ttcgacttca ccaactcagg tgaatccgtc ttcggcagtt
     5461 ccagctccta cgcaatatta agcgacgtta accctctccg cccggagagg gttagtagtt
     5521 ccagcgataa cgctgcaagt cgatttgccc gcttcccgat accatcacac cttctgccag
     5581 taatgcctgt cgctgacgct gtaaatccgg tccggttagc gaaattgtgc cgtggcgatt
     5641 aaccacccgg tgccagggta aggtgctgcc ttcagggaga cgctttaaca caccgcccac
     5701 ctggcgcgcg gcgcggggcg atcccgccag tttcgccaca tcaccgtaag tggtgacata
     5761 gccttcggga atagcggcga cgatttgcca gacgcgttgg ggaaatgaat cttctttttc
     5821 catcttttct tcctgaggta atttttcagc ataatctgga aaaacgcccg agtgaagtcg
     5881 cattgcgcaa gaaaccagca tctggcacgc gatgggttgc aattagccgg ggcagcagtg
     5941 ataatgtgcc tgcgcgttgg ttctcaacgc tctcaatggg ggctctgttg gttctcccgc
     6001 aacgctactc tgtttaccag gtcaggtccg gaaggaagca gccaaggcag atgacgcgtg
     6061 tgccgggatg tagctggcag ggcccccacc catttctgcc tcccaccgtt tcgtcaaaaa
     6121 aaaccaacat ggctaaactt taaccacaac tgacgtcgca agaattgtct ggctgcgcag
     6181 tacgcttcgg aggtatgtct gatgaagtat gttgatggtt ttgtggttgc cgttcctgcc
     6241 gataaaaagg atgcctatcg ggaaatggcc gctaaggctg cgccattgtt taaagagttt
     6301 ggcgcgcttc gtattgtcga atgctgggcc agcgatgtac cggatggcaa agtgaccgat
     6361 tttcgtatgg cggtgaaagc ggaagagaat gaagaggttg tctttagctg gattgaatac
     6421 ccttcaaaag aggtccgcga cgctgctaat caaaagatga tgtcggaccc acggatgaaa
     6481 gagttcggcg agtccatgcc gtttgacggc aagcgaatga tctatggcgg attcgagtca
     6541 atcatcgacg aataggtagc gtgacgggct gtgcggcgtg ccgtcagccc a
//
LOCUS       DAZPEB010000199         6415 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZPEB010000199 DAZPEB010000000
VERSION     DAZPEB010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6415
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.204"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..1000)
                     /locus_tag="ACHURJ_003590"
     CDS             complement(<1..1000)
                     /locus_tag="ACHURJ_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFR1282380.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DSYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQTLPGSVRSVAVLDRTKEPGAQA"
     gene            1274..1540
                     /locus_tag="ACHURJ_003591"
     CDS             1274..1540
                     /locus_tag="ACHURJ_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFR1282381.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            complement(1537..1959)
                     /gene="hslJ"
                     /locus_tag="ACHURJ_003592"
     CDS             complement(1537..1959)
                     /gene="hslJ"
                     /locus_tag="ACHURJ_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFR1282382.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(2070..3059)
                     /gene="ldhA"
                     /locus_tag="ACHURJ_003593"
     CDS             complement(2070..3059)
                     /gene="ldhA"
                     /locus_tag="ACHURJ_003593"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFR1282383.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPANYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            3267..5906
                     /locus_tag="ACHURJ_003594"
     CDS             3267..5906
                     /locus_tag="ACHURJ_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFR1282384.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVIAFEYQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTNINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            5903..6088
                     /locus_tag="ACHURJ_003595"
     CDS             5903..6088
                     /locus_tag="ACHURJ_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFR1282385.1"
                     /translation="MKILLAALTSSFMLAACTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLY"
     gene            6093..>6415
                     /locus_tag="ACHURJ_003596"
     CDS             6093..>6415
                     /locus_tag="ACHURJ_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFR1282386.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVR"
BASE COUNT         1592 a         1531 c         1701 g         1591 t
ORIGIN      
        1 ctgcctgggc accgggttct ttggttctgt ccagtaccgc cacgctgcgt acggatcccg
       61 gtagagtttg tagcaagtgt ttggctgaga aggggcggta caggcgaact ttcagcacgc
      121 cgactttttc gccacgggtt agcaattcat caaccacttc ttcacaggtg ccaatggctg
      181 agcccatcag gataatcacc cgttcagctt gcggatgccc gtaatattca aacggctgat
      241 actgacgacc tgtcgcggca gagaaatcat tcatcgcctg ttcaacatgg tcatagactg
      301 cgttgtacca ggggttggtg gcttcgcgag actggaaata agagtcagga ttggcggacg
      361 taccacggat caccggatgt tccgggttga gcgcccgggc gcgatgagca tcaatttcgg
      421 cctgcggcat gagatcaaga atcgtgtcat cggccagcgg gacaatttta ttgatttcgt
      481 gggacgtgcg gaaaccatca aagaaatgaa taaatggcac gcggcttttc agcgtcgcga
      541 tgtgcgaaat gagagcaaag tcttgcgctt cctggacgtt tgccgcacac aacatcgcgc
      601 aacccgtctg gcgcaccgcc atgacgtcgg aatgatcgcc aaaaatagag agtgcatgtg
      661 tggcaacggt acgtgccgct acatgcagga caaacggtgt gagttcgcct gccagtttgt
      721 acagtgtcgg gatcatcagc agcaaaccct gcgatgacgt aaacgatgtt gaaagggcac
      781 ccgtctgcaa agcgccatgc acggtagcga tagcacccgc ttccgactgc atttcaacca
      841 cgcgtggtgt gtctccccaa acgttcttca agccgtttcc ggcccaggca tcagcctgtt
      901 ctgccatcgt ggaactgggg gtaatagggt agatggcgat aacttcactg gtgcgaaacg
      961 ccaccgaagc aaccgcgcca ttaccgtcaa tagtaatcat atgacaccct tacattgcgc
     1021 aaatgagggg cgcacgaaat tgctgcgcgc ccagtagtaa tctttcaatt ttagcaaatg
     1081 gctttcttct gcattttcgc ttttgtgtcc cccacatcag cgtaatgaat gttttgatca
     1141 aacagagggc aaaaaaatag ccataaaaaa gtaaaaatgc ggggcaacgc gcaggacagc
     1201 tctcgatgtc gccctctgtg ctgcctatta tttatggcgg tgtcgtttgg cttgaagatc
     1261 agaggaagaa aagatgcgag cagcgttttg ggtagggtgt gccgctttat tgttgtcggc
     1321 gtgtagtagt gaacctgttc agcaggcgac tgcggcgcac gtagcgccag gtttaaaagc
     1381 gtcgatgtcc agtagtggag aagcaaattg tgcaatgatc ggcggttcgc tttctgttgc
     1441 ccgtcaactg gatggcacgg cgattgggat gtgtgcatta cccaacggca aacgctgtag
     1501 cgaacagtca cttgccgccg ggagctgtgg cagctattaa ttcattaaat ccgccagctt
     1561 ataagttaat gtctgtttcg cggtcgccag cgttaactgg ttcgcggtca gatccacttg
     1621 tgcaccttct ttcagcattt cgctaatggt gttatcgagt tcattaagct gcgggttagc
     1681 gcacatcata cgggtcattg ccagcccttt ggctgtcagt tcaccattag acagtttgcc
     1741 ttcaccgcta aagcggttac acatgctgcc ggaaatcatc attttttcac caaaactgat
     1801 ttctggcgga tttttatcgc tggtcacggg cttaccgttt acgctttcca gcacaaagcg
     1861 atgatgctgt agctgttctg gcgtaacagc aattttgtca ttacttacac atcccgccat
     1921 cagcaggctt agcgcaacaa acgcggctac tttcttcatt gtggttctca attacagttt
     1981 ctgactcagg actattttaa gaatagagaa tgaaaggtca ttggggatta tctgaatcag
     2041 ctcccctgga ttgcagggga gctgcaacat taaaccagtt cgttcgggca ggtttcgcct
     2101 ttttccagat tgcttaagtt ttgcagcgta gtctgagaaa tactggtcag agcttctgct
     2161 gtcaggaatg cttggtgccc ggtaaacagc acgttgtggc aggcagacag gcgacggaat
     2221 acgtcatcct ggatcacgtc gttggattta tcttcaaaga acagatcgcg ttcgttctca
     2281 tacacgtcca tacccaacga accaattttc tgatttttca gcgcttcaat tgctgcctga
     2341 gaatcaatca atgcaccgcg actggtattg acgatcatca cgccattttt catctgatcg
     2401 aaggcggctt cgttcaacag atggtagttt gccggtgtca gcgggcagtg cagagagata
     2461 acgtctgatt cagagaacag ggttggcaga tcgacatact ccacaccgag ttccagcgcc
     2521 gctgcacttg gatacggatc gaacgccagc agacgcatac caaaaccttt cagaatgcgc
     2581 agcatcgcca caccgatttt accggtaccg ataacgcctg ccgttttgcc atacatagta
     2641 aagccggtca gaccttccag agagaagtta gcgtcacggg tacgctgata cgcgcggtga
     2701 atacggcggt tcagcgtcat catcataccg atggcgtgtt cagcaacggc ctctggatca
     2761 taggctggaa cacggactac tttcagcccc agttcttttg ccgcatcaag gtcgacgtta
     2821 ttgaaaccgg cacagcgcag ggcgatatat ttaacgccgt gctttttcag ctcttccagc
     2881 accgggcggc tgccgtcatc gtttacgaaa atacataccg cttcgcagcc attggcagtt
     2941 ttagcggttt tttccgtcag cagaaagtca aaaaattcca gctcaaagcc aaaggactcg
     3001 ttcacctgtt gcaggtactt cttgtcgtac tgttttgtgc tataaacggc gagtttcata
     3061 agactttctc cagtgatgtt gaatcacatt taagccacta aaaatatttt acaaaatttc
     3121 aaatataatt gaaaaccatg gcgatattga aaaattcatc aacaactatg cttagtgtag
     3181 gcgcaacctt caactgaacg gttaaacatg ccacaatacc cgtattgaat gcttaatttt
     3241 tcgctaaatc aggatattaa ctacccatgc tgggtaaata taaagccgtt ctcgcgctgt
     3301 tattactgat tattcttgtg ccgttgacgc tgctgatgac gctcgggctg tgggttccca
     3361 cgctggcggg catctggcta ccgctcggga cacgtattgc attagatgaa agcccacgca
     3421 ttacgcgtaa aggtttaatc attcccgatc tccgttatct ggtgggagat tgtcagcttg
     3481 cacatatcac caacgccagc ctttcacatc ccagccgctg gttattgaac gtcggcacgg
     3541 tagaacttga ttctgcttgt ctggcgaaat tgccgcagac ggagcaatcg ccagctgctc
     3601 caaaaaccct cgcgcagtgg cagtccatgc tgcctaacac ctggatcaat atcgataaac
     3661 tgattttttc tccctggcag gaatggcagg gaaaactctc tctcgcatta acctctgata
     3721 tccagcaact gcgttatcag ggcgaaaaag ttaaatttca aggccagctg aaagggcaac
     3781 aacttacagt cagcgaactg gatgtcatcg cgtttgaata tcagccgccg gttaaactgg
     3841 tgggggaatt tactatgccg ctcgtgccgg atggacttcc tgtaagtggg catgctactg
     3901 cgacgttaaa cttgccgcag gaaccgtcac tggtggatgc agagctggac tggcaggaaa
     3961 atagcgggca attgattgtg ctggcacgtg ataacggcga tccgttgctc gatttgccgt
     4021 ggcaaattac tcgtcaacaa ttgaccgtaa gcgatggtcg ctggagttgg ccgtatgcag
     4081 gttttccttt gagtggccga ctgggtgtca aagtcgacaa ctggcaggca gggcttgaga
     4141 acgctctgat cagcggacga ctgagtgtgc tgacccaggg gcaagcgggt aaaggcaacg
     4201 cggtgctaaa ttttggccca ggaaaattaa gcatggataa cagccagctg cctctgcagc
     4261 tgaccggtga agcgaaacag gcggacctca ttttatatgc ccgcttaccc gcgcagctaa
     4321 gtggaagtct gactgaccca acgctggcct ttgagccagg cgcgttactt cgttcaaagg
     4381 gaagagtcat cgattcgctg gacatcgatg aaatccgctg gcctttagcg ggtgtaaaag
     4441 tcacccaacg tggtgttgac ggacgtttgc aggccatttt gcaggcgcat gaaaatgaac
     4501 tgggcgattt cgtgctgcat atggatgggc tggcgaatga ttttctccct gacgcgggcc
     4561 gctggcagtg gcgctactgg ggaaaaggca gttttacacc gatgaatgcc acctgggatg
     4621 tcgcaggaaa aggtgaatgg catgacagca cgattacgct gaccgacctc tccaccggtt
     4681 tcgaccagtt acaatacggt acgatgacgg tagaaaagcc gcgattaatt ctggacaagc
     4741 ccgtcgtctg ggtacgtgac gcacagcatc cctccttcag cggcgcgctg tcactggacg
     4801 ccgggcaaac gctgttcact ggcggcagtg tgttaccgcc atcaacctta aaatttagcg
     4861 tcgatgggcg cgatccgacg tatttcctct ttaaaggcga tttacatgct ggtgagattg
     4921 gcccggttcg ggtaaatggt cgctgggacg gtattcgtct gcgcggtaac gcctggtggc
     4981 caaaacaatc actgactgta ttccagccgc tggtgccacc cgactggaag atgaacttac
     5041 gcgatggtga actgtatgct caggttgcat tttctgctgc gcctgaacaa ggattccgcg
     5101 cgggagggca cggcgtgttg aaaggcggta gtgcctggat gccagataat caggttaacg
     5161 gtgtcgattt tgtcctgcct ttccgttttg ccgatggagc ctggcatctg gggactcgcg
     5221 gccccgttac gttgcgaatt gccgaagtga ttaatctggt gacagcgaaa aatattacgg
     5281 ctgatttgca agggcgttat ccgtggactg aagaagaacc cttgctgttg actgatgtta
     5341 gcgtcgatgt gttaggcggt aacgtactga tgaaacaatt acgtatgccg caacatgacc
     5401 cggcgctgtt gcggctgaat aatctctcat ccagcgaact ggttagcgcc gtcaatccga
     5461 aacaattcgc catgtccggg gcatttagtg gtgcattgcc gttatggcta aacaacgaaa
     5521 aatggatagt gaaagatggt tggctggcaa atagcgggcc aatgaccttg cggctggata
     5581 aagacaccgc agatgcggtg gtgaaagaca atatgactgc gggttcagca attaactggt
     5641 tgcgctatat ggaaattagc cgttcatcga caaatattaa tttagataat ctcggtttat
     5701 taaccatgca ggccaacatt acaggtacca gtcgcgttga tggtaaaagc ggtacggtaa
     5761 accttaatta ccatcatgaa gagaatattt ttacgctgtg gcgcagttta cgctttggcg
     5821 ataatctcca ggcatggttg gagcagaacg cacgtctgcc gggaaatgac tgtccgcaag
     5881 gaaaagagtg tgaggaaaaa caatgaaaat tttactggct gcgttgacgt catcttttat
     5941 gctggctgcc tgtacgccgc gtattgaagt cgctgcgccc aaggaaccga tcactatcaa
     6001 tatgaacgtt aaaattgagc atgagatcat catcaaggca gacaaagatg tcgaagagct
     6061 gcttgaaact cgtagcgatc tttactgagg cgatgatgaa gaaaacatta cttctttgtg
     6121 cgtttcttgt tgggctggta agcagcaatg taatggcatt gactctggat gaagccagaa
     6181 cgcaggggcg ggtcggggaa acattttatg gttatctggt tgcgctgaaa acggatgctg
     6241 aaacagagaa attagtaacc gacattaatg ccgaacgtaa agcgagttac caacaactgg
     6301 caaagcaaaa taatgtgtct gtagatgata tcgcgaaact cgccgggcaa aagctcgtag
     6361 ctcgggccaa accgggggaa tatgtacaag ggataaacgg taaatgggta cgaaa
//
LOCUS       DAZPEB010000200         6390 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZPEB010000200 DAZPEB010000000
VERSION     DAZPEB010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6390
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.205"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..206)
                     /locus_tag="ACHURJ_003597"
     CDS             complement(<1..206)
                     /locus_tag="ACHURJ_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFR1282387.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRD"
     gene            685..2462
                     /locus_tag="ACHURJ_003598"
                     /pseudo
     CDS             685..2462
                     /locus_tag="ACHURJ_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
     gene            complement(2502..2981)
                     /gene="xdhC"
                     /locus_tag="ACHURJ_003599"
     CDS             complement(2502..2981)
                     /gene="xdhC"
                     /locus_tag="ACHURJ_003599"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFR1282388.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(2978..3856)
                     /gene="xdhB"
                     /locus_tag="ACHURJ_003600"
     CDS             complement(2978..3856)
                     /gene="xdhB"
                     /locus_tag="ACHURJ_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFR1282389.1"
                     /translation="MFDFASYHRAATLADAINLLANNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAAMSIAG
                     LQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQMMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(3867..6164)
                     /gene="xdhA"
                     /locus_tag="ACHURJ_003601"
     CDS             complement(3867..6164)
                     /gene="xdhA"
                     /locus_tag="ACHURJ_003601"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFR1282390.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAIAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGTFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
BASE COUNT         1595 a         1511 c         1614 g         1670 t
ORIGIN      
        1 ttgtcgcgga atacggaaat acctaatcca ctattaaaga ctttggttga gatgttttca
       61 gcacgcagtg cttttaatgc tgcggcaacg tccagtactt gtttcagttc atctggcgtc
      121 tgttcccacg ttaacaaaaa atctttctcg tgaaggtgag aggtcagcga attgatatcc
      181 ttaatcagct cattaacagt tttcatcata aagtcccggt agtggaatga caggcaaagt
      241 ggtttatttc actgtgactt gccaaagtga ttggtgtttt cacagctata cctgggacta
      301 acaataagtg tgccagaatt atccgcgcgg ctgcaaaaga ctaatttgac ggataatgtg
      361 tggggttgat cacaaattga tgccagcttc tggttgtttg taaaatacga aaacgtttgg
      421 ctttttttat cgcgttattt atttgctgct aagtatcaga aataagataa aggggcaggc
      481 tatctctctt cagtttgata aactcaccgc cgtaattctc atattgatat agaaatgtga
      541 tatctcttcc ataatttcat tagaaaatga taaaaatctt atgtttttat caaagccaat
      601 ttcgcctgag ttaatatcat catgatctgt gaaccatcaa cgtcttcaat gatcgacttt
      661 taagtaagtc tggaaggtaa agctatggag cttgctacta cgcagtcagt attgatgcaa
      721 attcaaccga caattcagcg ttttgccaga atgcttgcca gcgttttgca gcttgaggtt
      781 gagatcgttg atgaaaactt gtgtcgcgtc gccggaacgg gcgcgtatgg gaagtttctt
      841 ggtcgccagt tgagcggcaa ctcacgcctg ctccgccacg tcctggaaac gaaaactgaa
      901 aaagttgtga cacagtctcg cttcgatccc ctttgcgaag gttgcgatag taaagaaaat
      961 tgccgtgaaa aagcatttct gggtacgcct gtcattttac aggatcgttg tgttggggtg
     1021 ataagtttga ttgccgttac ccacgagcaa caagagcata tcagtgataa tttacgcgaa
     1081 ttttctgatt atgttcgcca tatatccacc atttttgttt cgaaacttct ggaggatcag
     1141 gggccaggag ataacatcag taaaatattc gcgaccatga tcgataatat ggatcagggc
     1201 gtattagttg ttgatgatga aagtcgggtt cagtttgtta atcagactgc cttaaaaaca
     1261 cttggtgttg tacaaaataa tattattggg aaacctatcc gtttcagacc attaacattt
     1321 gagagtaatt ttactcatgg acatatgcag catattgttt cgtgggacga taaaagtgaa
     1381 ttaatcattg gtcaattgca taacattcag ggccgacaat tatttttaat ggcatttcac
     1441 caatcgcata ccagtttttc tgtagcaaat gcacctgatg aaccacatat tgaacaattg
     1501 gttggcgagt gccgtgttat gcggcaatta aaacgactca ttagccgtat tgcacccagc
     1561 ccatccagcg ttatggtggt tggtgaaagc ggcacgggta aagaagtcgt cgcccgagca
     1621 atccataagt tgagcggaag acggaataaa ccctttattg ctatcaactg tgccgcgatt
     1681 ccggagcagc ttctggaaag cgaactgttc ggttatgtta aaggcgcatt tactggcgct
     1741 tctgccaacg gtaaaacagg gttgattcag gcggcgaata cgggcacgct gtttctcgat
     1801 gaaataggtg atatgccatt aatgttgcag gctaaattac tgcgcgctat tgaggcgcgt
     1861 gaaattctgc cgattggtgc cagtagccca atacaagtcg acattcgcat catttctgca
     1921 actaatcaga atttggccca gttcattgcc gaaggtaaat tccgcgaaga tctcttctac
     1981 cgacttaatg ttatcccgat aactctgcca ccgctgcgtg aacgtcagga agatattgaa
     2041 cattggtgca ttacttttta catctgcata cccgtcgtct gggatcggtt tatcctggca
     2101 ttgctcccga tgtcgtcgaa atattgcgta agcatcgttg gcccggaaac ctgcgcgagt
     2161 taagcaattt gatggaatat ctggttaacg tagttccttc aggtgaagtt atcgacagca
     2221 cgctattgcc gccaaatctg ctgaataatg gcacaacgga gcaaagtgat gtaacagagg
     2281 tcagtgaggc gcacctgtca ctcgatgatg cgggcggcac ggcgctggag gagatggaaa
     2341 agcaaatgat ccgcgaggcg ctttcacgtc ataacagcaa gaagcaagtt gctgatgaac
     2401 tgggcatcgg cattgctacg ctctatcgca agattaagaa atatgagttg ttaaacacat
     2461 aacctcagtg aatccggcct gaattcaggc cggatatcct tttacttcgt tttctcgcaa
     2521 tccagaactg tatttacaat catctgatac cccgtgcagc gacaaagatt tcccgccagt
     2581 ccgcgacgaa tttccgtaat ggttaatggc ttctcgcgtg gtttcgccag cattgccgtg
     2641 gtagccataa tcaggccagg cgtacaaaac ccgcactgca ctgcgccgga tttcgcataa
     2701 gcctgctgaa catgagaaag ttttccgcct ttcgcttcac cttccagcgt gcggatctct
     2761 tttccttcag cccaggcggc aaggtataag caactgtcta ttgctgtgcc gtcgaccaac
     2821 accgtacagg caccacattc acccacgcag cacccttgtt tgacacttag cagtccttgt
     2881 tcgcggagta attccgagag cggcgtgcct ggtgcggcgt gaagctgaaa aggcatcccg
     2941 ttaatggtgc attcgatggt aattgtttcg ctgtgattca ttgcaatttt ccccccgccg
     3001 cggcgacggc ttcgctaatc acttttttgg tcatcatctg gatgagatgc agacgaaact
     3061 ctttactggc ccgccatgaa gaacgcgggg cgacatcttg caggacagat tcgctgatag
     3121 cttccagcgt ttgcaggttt aatggcgcat tttgtgcagt ctgttcggca tgttggcagc
     3181 gaatcggcgt tggcgcggca acaccaaatg ccaggcgtaa ttcgctgaaa ttgccgttat
     3241 ccagtcggca atgtgcggcg cagccaatcg ttgaaatatc cattgcgtcg cgcatggcat
     3301 atttaaaatg cgcgctgccc gcgtgttctt tcggctgtgg cggaaaatga aaggcgacga
     3361 ggatttcgtc atgctcaaga gacactttgc ccggcccggt gtgaaagcca ttaatcggga
     3421 cgaaacgaac accgcgcggg gagtggatct ccagtttcgc gtcataaatt agcgttggcg
     3481 tggcagaatc tgcgctggtg gcaccgttgc aaatatttcc accgtaggta gcgacgttac
     3541 ggatctgcag tccagcaatg gacatggccg cagcacataa cgccgggaga tgacgttgag
     3601 ttataggatc ttctattagc tgggtaaatg tcgttgcaga gccgatacgt agcgagccat
     3661 cttccgccag cgtaattccc cgcagctccg ccagattatg aatatcaaca atatggcgat
     3721 aacggtcatt gtgatggtgg agctgaatca gtacgtcagt gccaccggcg agcagtttgg
     3781 cctgcgggtt gttagccagc aggttgatgg catcggcaag ggttgctgcg cgatggtaag
     3841 aagcaaaatc aaacatgatg ttatcctcaa atcaatcctg ccagatggaa ctcttcatat
     3901 aaccgttttg gcgtcagcgg cagtgtattg attgcaacac cggtagccat cttcaccgcg
     3961 ttacgaatag cagcggcaac aggaattatt ggtggctcac ccagtgactt atgtccgtat
     4021 gcggattgcg gctcattgat ttcgacgaac gcgctttcca gttgtggcag atccggcatg
     4081 gtcggcattt tgtaatccag cagattgggg ttacggacca cgccgctttt agcatcgatg
     4141 atcatctctt caaatagcgc ccagccaatg cccattccca ttccgccgtg tacctgacct
     4201 tctgccagca gtggattaag aatatgccct gaatcatgaa cgttgaggat gcggttgatg
     4261 gtgactttgc acagcgcaat atcgaccgtc agatcaacaa aggtacagcc aaacgccggt
     4321 gggttagtgg tggttttgat ggagctttca gcagagagct gcccgccgcg ttcagggtgg
     4381 tagaaagcgt ccatcgccaa atcttttaac gacattaacg gctcttccgg tcgttcaacc
     4441 agcacgatat ggccttttat cagggtcaga ttcatcgctg actgatgtag catgactgcg
     4501 gcgtgagcga tgattttctc ttttaataat agtgccgcac tgcgcagcgc aggcgcggca
     4561 acatagctct ggcgtgaggc aaatgtgccg ggatcgaacg gcgtaacgtc ggtatcttga
     4621 gttgaaataa cgcgaacgtc gctgaccgga acccccacgg tttctgccac catttgcgag
     4681 aagacggtgt cggcaccctg accgatttcc gtcgcgccgc tttgcacgtt gatggttcca
     4741 tcctgattca tcagaaggcg cgcgcctgct atttctacgc cgacaggcca ggtgttagag
     4801 gtgtagctaa aacaggcgac gccaacgccg cggcgcaaat tgccttgctg gttctggcat
     4861 tctgcacggc gtttttccca ttcaaagatt ttccggcctt tttcaagaca ctccggcaac
     4921 cctgcgctgt aaatacgttt gcccgtgagc ggattagcat ctccttcgcg ggcggcgttg
     4981 cgtaaacgaa tttcaacagg atcaatacct aacgctgtcg cggcgtcatc aagcatagac
     5041 tcaacggcaa atacgacttg tggcgcgcca taaccacgca tcgcaccagc cgaggggagg
     5101 ttggtatagc aggtctttga actgtaagcg taggcacaac gaggataaag gtaagcgact
     5161 ttattccccc ccgcagaagc gatggagtgc ccgtgagatg cataagcgcc ggtgttagac
     5221 agaacatcca gactataacc tttcaatgtt ccgtcgcggt tcacgcccat ttgcccgtca
     5281 atggtaaaag cgtggcgggt acgggttgcg aggaaacact cttcacggct aagggaaact
     5341 ttcaccggaa tgccgccaag cttgctggtc aggaatgccg ccattggctc ttccagtaca
     5401 tcctgtttat taccaaaacc gccaccgaca aatggtttga tgactcgtac gcatgaccag
     5461 ggaatatcca gcgcctgacc aaccacgcgg cgaacaatgt gcgggatctg ggtgctggaa
     5521 acgatggtaa ttcgcgagtc atcctccatc cacgccagcg atgttacgct ttccatgtga
     5581 caatgttgaa taacgggggt ctgatagtgc ccctgtacct ggtagtcggc ggcatcgatt
     5641 gtttgttgga cattacccgt cgacatcgtg ctttgtttca gtaaattgcc accgttatgg
     5701 attggtgcag cgtcttctgc cagcgccgct tctggcgtgg tgataacggg taattcttgc
     5761 cactcaatgc tgaccaattg cgccgctttt tctgccgtga gttcatcgcg ggccacgacg
     5821 atggcaatgg cgtcaccatg atgacgaaca tggcgagtta gcagtgcgcg atcggcggta
     5881 tcgcgcttgt tttcgtcaag tgtccaggca tgcccagctg tagcgaatgg aatatcaggc
     5941 acatcttccc aggtaaaaat cgccagtacg cccggcaaac ttctggcttg ttcatcatta
     6001 atacttacgg cataaccatg tgcgataggg ctacgtacat atttcgcata acacatgccc
     6061 gccataacat aatcgtcagt atatcgtgcc cgcccggtga ccttagcaat ggcatcgacg
     6121 cgcatgcatg attcacccgt agcggttgct tcccgcgctt ccatagaaat cccctcgatt
     6181 tgaacatttg gttcttatgg caaagattta gtctaagcag gcaagattta cgccagaaac
     6241 gacgataagc gttaggggag gtaggaagtg tgatgacgtt aaaaaaacac agatatgata
     6301 cgaaacagga gtctgaatag gggcagtgat acgtatcaat atgagtggcg ataaaatcaa
     6361 tgtgataaat tgggattatt acgcatcagg
//
LOCUS       DAZPEB010000201         6345 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZPEB010000201 DAZPEB010000000
VERSION     DAZPEB010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6345
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.206"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="ACHURJ_003602"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="ACHURJ_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR1282391.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="ACHURJ_003603"
     CDS             2219..2767
                     /locus_tag="ACHURJ_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFR1282392.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="ACHURJ_003604"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="ACHURJ_003604"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFR1282393.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3663..4853)
                     /gene="aspC"
                     /locus_tag="ACHURJ_003605"
     CDS             complement(3663..4853)
                     /gene="aspC"
                     /locus_tag="ACHURJ_003605"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFR1282394.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARMAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(5038..6126)
                     /gene="ompF"
                     /locus_tag="ACHURJ_003606"
     CDS             complement(5038..6126)
                     /gene="ompF"
                     /locus_tag="ACHURJ_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFR1282395.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         1599 a         1560 c         1608 g         1578 t
ORIGIN      
        1 taacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgccgg tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgcgac gtagtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccggtcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcacttg caacgccgcc gctctcggtg attaaccaat ggcagctggc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggcctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gcgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggggcaga
     1201 tggtgctatt ggcccggcaa cacgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggct ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccggcct attcgctggt ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcccttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt acctccaact ctggcacgca aagatattct
     1501 gccaaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgtgg
     1561 ctggaacagc agagaagcga ttgacccatg gcaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg ttccgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggctg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atggtcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca gttgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga cctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttccatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattacccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagcaa tcgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attaatctgg tgtgaacaaa cccgtctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata ggaaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc cacacttggt tatgaacgcc tgcataatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aggcacataa aaaagcccgc ttttaatgcg ggcctggatt
     3481 tctggcaaag tgcgctttgt ttatgccgga tgcggcacga gcgccttatc cggcctacaa
     3541 aatcgtgcaa attcaaaata ttgcagggga cgcgtaggcc tgataagcgt agcgcatcag
     3601 gcaatgttgc gtttgtcatc agtctcagcc cgcttttcag cgggcttcat tgtttttaat
     3661 gcttacagca ctgccacaat cgcttcgcac agcggagcca tgttatctgg tgtcatcccg
     3721 gccacattta cgcgaccaga agcaaccgca tatacgccaa actcttcgcg cagacgcagc
     3781 acttgttctt ttgtcaggcc actgaaggag aacatgccgt tctgtttgat gataaagctg
     3841 aagtcgcggt ttgcgccttt ttcctgcagc gtattgacga acaactgacg catacgctga
     3901 atacgctggc gcatatcagt cagctcttgt tcccaaatcg cacgtaacgc atcgttgctc
     3961 aggatggtgg caacaacaga agcgccgtgt gctggtgggt tagagtagtt agcgcgaatc
     4021 gccgctttca tttggctgaa tgcgcgatca acggtttcac tgtcggcagc aaccagagta
     4081 caagcgccaa cacgctcgtt gtacaggcca aagtttttag agtaggaact ggcaacaatc
     4141 agctctttat gcatagccgc gaaagcgcgc agtccttcag catcttcttc cagaccacgg
     4201 gcaaaaccct ggtaagcgaa gtcaaacagc ggtaaccagc ctttctcaac ggagagttgt
     4261 gccagtgtct gccattgttc cagcgtaggg tcgataccgg ttgggttatg gcagcagcca
     4321 tggaacagca ctacgtcgcc agcctgagct tcattcaggc tgttaatcag tgcatcgaag
     4381 tcaagagtgt gattttccgc atcataataa gcgtattcac gaacttccag acctgcagag
     4441 ttaaagacgc tcttatggtt cggccagctt gggttgctca cccacactcg cttaacgctg
     4501 gtattttttg ccaggaaatc ggcagctacg cgtagtgcgc cagtgccccc cggagtctgt
     4561 gccatgcgag cacgtttgtc attgatcagg gcgctacctt taccaaacag cagttcctga
     4621 gtgcagcgac caaattcagg gatgccgtca atgccgaggt aatttttggt ggtttcattt
     4681 tcgagcagat actgttcagc ctttttcacg ctggtcagta ccggggtttt gcccgtctca
     4741 tctttataga caccaatccc gaggttaatt ttgccgggac gttcatcggc acgaaacaga
     4801 tcggccaggc ccagaatcgg gtcggcagga gcggcggtaa tgttctcaaa catgacgagg
     4861 ttccattatg gttacagaag ggaagtccgc tatcagggta acgggagatt tacaaaattc
     4921 caactattac tgatgaaaac gcaggctgtt tttgcaagac gtgagattgc tctggaaggt
     4981 ataaaaaaaa caggaccaaa gtcctgtttt ttcggcattt aacaaagagg tgtgctatta
     5041 gaactggtaa acgataccca cagcaacggt gtcgtctgaa cctacgccca gtttgttatc
     5101 agaatcgatc tggttgatga tgtagtcaac ataggtggac atgtttttgt tgaagtagta
     5161 ggttgcgccc acttcaaagt agttcaccag atcaacatca ccgatacctt ctacgtcttt
     5221 cgctttagat ttggtgtaag cgatggacgg acgcagaccg aaatcgaact ggtattgcgc
     5281 aactaacaga acgtcttgcg ttttgttggc gaagccgctg gtgtttgtaa atttattagt
     5341 gatcggcgta gcgttacggg tttcaccgta gttcgctgcc aggtagatgt tgttcgcgtc
     5401 gtacttcaga ccagtagccc actgttcagc ttttttaccg ttgccaagag gttgagcttc
     5461 ttgcaggttg gtacggtcag ctgcaccata agcaccaacg ataccaaagc cttcgtattc
     5521 gtagctgata gaaccgccaa caccgtcgcc gttagaacgg cgtgcagtgt cacgctcgtt
     5581 tttacccagg tactgaacag cgaagttcag gccatcaacc agaccaaaga agttggagtt
     5641 acgataggta gcaacgccgc caacacgacc aacgaagaag tcatcgctgt atgcagtatc
     5701 accaccaaat tctggcagca tatcggtgta acccagtgca tcataaacca caccgtagtt
     5761 acggccgtaa tcgaaagaac caacgtcagc gtatttaaga cccgcgaatg ccagacgcgt
     5821 tttgttacca gtttgagcgt cagcgccttc agagttgtta ccttggaagt tatattccca
     5881 ctgaccataa ccggtcagat cggaattgat ttgagtttcc cctttaaaac caagacgggc
     5941 ataggtcatg tcgccattgc caccgtaact gttttcaccg ttacccttgg aaaaataatg
     6001 cagaccaaca gctttaccgt acagatctac tttgttgcca tctttgttat agatttctgc
     6061 agcgtttgca gtacctgcta ctaacagagc agggacgatc actgccagaa tattgcgctt
     6121 catcattatt tattaccctc atggtttttt ttatgacacc tgccactgcc gtcaataagt
     6181 tctgtcaata aaaatttacg gaactattga tgagagtttg gtgtctttat gtgtctgcag
     6241 gcatctttcc attcaaacta acgtttcgct accgtgaaag tgctgcaaag ataaagattt
     6301 agtttcaaaa agaaaaaata tgcaaccaaa agtaaaaatt aagga
//
LOCUS       DAZPEB010000202         6337 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZPEB010000202 DAZPEB010000000
VERSION     DAZPEB010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6337
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.207"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            30..1013
                     /locus_tag="ACHURJ_003607"
                     /pseudo
     CDS             30..1013
                     /locus_tag="ACHURJ_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
     gene            1036..2547
                     /locus_tag="ACHURJ_003608"
     CDS             1036..2547
                     /locus_tag="ACHURJ_003608"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFR1282396.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            2572..3570
                     /locus_tag="ACHURJ_003609"
     CDS             2572..3570
                     /locus_tag="ACHURJ_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1282397.1"
                     /translation="MSASSLSLPLGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            3603..4697
                     /locus_tag="ACHURJ_003610"
     CDS             3603..4697
                     /locus_tag="ACHURJ_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFR1282398.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALRSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            4709..5581
                     /locus_tag="ACHURJ_003611"
     CDS             4709..5581
                     /locus_tag="ACHURJ_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFR1282399.1"
                     /translation="MTIYTLSHGPLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(5629..6051)
                     /locus_tag="ACHURJ_003612"
     CDS             complement(5629..6051)
                     /locus_tag="ACHURJ_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFR1282400.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIILGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(6148..>6337)
                     /locus_tag="ACHURJ_003613"
     CDS             complement(6148..>6337)
                     /locus_tag="ACHURJ_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase C-terminal domain-containing
                     protein"
                     /protein_id="HFR1282401.1"
                     /translation="WLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSG
                     KTFDAKLLIDENIALKSL"
BASE COUNT         1467 a         1515 c         1835 g         1520 t
ORIGIN      
        1 ttgttaataa aaaccctaca ggaggcacta tgcctaaaaa aatgagaaca acccgtaatc
       61 tattattaat ggctacgcta ttaggtagcg cactatttgc cagggctgcg gataaagaaa
      121 tgaccattgg tgcaatatac cttgatacct agggatatta cgctggagtg cgtcagggcg
      181 ttcaggatgc ggcaaaagat tcttcagtac aggtacagtt aattgaaact aacgcccagg
      241 gtgatatttc gaaagaaagt acctttgttg ataccctcgt ggcgcgtaat gtcgatgcca
      301 ttattttatc ggcagtgtct gaaaatggca gtagccgtac cgttcgtcgc gccagtgaag
      361 cgggtattcc ggtgatttgc tacaacacct gtattaatca aaagggtgtc gataaatatg
      421 tctcggcgta tctggtcggc gatccactgg aatttggtaa aaaactgagt aacgctgccg
      481 ccgattattt tattgccaat aaaattgacc agccgaaaat tgccgtcatc aattgcgaag
      541 cctttgaagt ttgtgtgcag cgacgtaaag gatttgaaga agtattaaaa tcccgcgttc
      601 ccggcgcgca aattgtcgct aatcaggaag ggactgtttt agataaagcg atttccgttg
      661 gtgaaaaact gattatctcc acgccggatc tcaacgccat tatgggggag tcgggcggtg
      721 cgacactcgg cgcggtaaaa gcggtacgta atcaaaatca ggccggaaaa attgctgttt
      781 tcggttcgga tatgacaacc gaaattgctc aggagctgga aaacaatcag gtgctgaaag
      841 cggtagtgga tatttccggt aagaaaatgg gcaatgctgt tttcgcgcaa acattgaagg
      901 ttatcaataa acaagccgac ggtgaaaaag tgattcaggt gccaatcgat ctctatacca
      961 aaacggaaga tggtaaacag tggctggcaa cgcacgttga tggtctgccc taatcgctga
     1021 ttcgcagagg tccctatgtt cacggcaaca gaggcagtcc cggtagcaaa agtggtggca
     1081 ggaaataagc gttaccccgg cgtcgttgcg ctggataacg ttaacttcac gctcaataaa
     1141 ggcgaagttc gtgcgctgtt aggtaaaaac ggcgcgggca aatcgactct cattcgaatg
     1201 cttaccggca gcgaacgccc ggatagcggt gatatctgga ttggcgagac gcgactggaa
     1261 ggtgacgaag ctacgttgac tcgccgtgcc gctgaactgg gggttcgtgc ggtttatcag
     1321 gaattaagtc tggtggaagg gctgacggtg gcagaaaacc tctgccttgg tcaatggcct
     1381 cgccgcaacg gcatgattga ttacctgcaa atggcgcagg atgcccaacg ttgcttacag
     1441 gcgctgggcg ttgacgttag tcctgaacaa cttgtttcaa cgctaagccc ggcgcaaaag
     1501 caactggtgg aaattgcacg ggtgatgaag ggcgagccgc gcgtggtcat tcttgatgaa
     1561 cccaccagct cgcttgccag tgcagaagtt gaactggtga tcagtgcggt gaaaaagatg
     1621 tcggcactgg gcgtggcggt gatttatgtc agccaccgga tggaagaaat ccgccgcatt
     1681 gcctcctgtg ccaccgttat gcgcgatggt caggtggcgg gcgatgtgat gctcgaaaac
     1741 acctccacgc atcatattgt gtcgctaatg ctagggcgtg atcacgttga tattgcgccg
     1801 gttgcccctc aggaaattgt ggatcaggcc gtactggaag tccgtgcgtt acgccataag
     1861 cccaagctgg aggatatcag ctttacgtta cgtcgcggcg aagtgcttgg cattgctggc
     1921 ctgctggggg cagggcgcag tgaattgctg aaggcgattg ttgggctgga gacgtatgaa
     1981 cagggcgaaa ttgttatcaa cggcgagaaa atcacgcgcc ccgattacgg cgacatgctg
     2041 aaacgcggca ttggctatac gccagaaaac cgcaaagaag cggggatcat tccctggttg
     2101 ggcgttgacg aaaatacagt gctgaccaat cggcaaaaaa tcagcgccaa cggagtgctg
     2161 caatggtcca ccatccgccg cctgaccgaa gaggtgatgc agcggatgac ggtcaaggcc
     2221 gccagtagcg aaacgcccat cggcacgctt tctggtggca atcagcaaaa agtggtgatc
     2281 ggtcgttggg tctatgccgc cagccagatt ttgttgctcg acgagccaac gcgcggcgtc
     2341 gatatcgaag ccaaacagca gatttaccgt attgtccgtg agctggctgc cgaaggaaaa
     2401 agcgtggtgt ttatctccag tgaagtggag gagttgccgc tggtgtgcga ccgcatcctg
     2461 ttgttacagc acggtacgtt ctcgcaggag tttcactcgc cggtcaatgt ggatgagctg
     2521 atgtccgcca ttctgtctgt gcactgatga attttaccga ggaacctgaa gatgtctgct
     2581 tcgtcattat cattgccgct gggcaagagc gtctcgctca aacaatttgt cagtcgccat
     2641 attaatgaga tcggtttgct ggtggtaatc gccattcttt atctggtctt ctccctgaat
     2701 gcgcctggct ttatctcatt gaataaccag atgaacgtgc ttcgcgatgc tgccaccatc
     2761 gggatcgccg cctgggcaat gacgctgatt attatctccg gtgaaattga tgtcagcgtt
     2821 ggaccgatgg tggcttttgt ctcggtgtgc ctggcatttt tgctgcaatt tgaagttccg
     2881 ttggcgattg cttgtctgct ggtgttgctg ttaggcgcgc tgatgggaac gctcgccggg
     2941 gtgctgcgcg gcgtgtttaa cgtgcccagt ttcgttgcca cactggggct gtggagcgcc
     3001 ctgcgcggaa tggggctgtt tatgaccaac gcactgccag tgccgattga cgaaaacgag
     3061 gtgctggact ggctgggcgg acaatttctc ggtgtgccag tatccgcgct gatcatgatg
     3121 gtgttgtttg cgctgtttgt gttcattagc cgaaaaaccg ccttcgggcg ctcggttttt
     3181 gccgttggcg gtaatgccac ggcggcgcag ttgtgcggta ttaacgttcg tcgagtgcgc
     3241 attcttatct ttaccctttc gggattatta gcggcggtga ccggcatttt gctggcggca
     3301 cgcctcggtt ccggtaacgc aggtgccgca aacggtctgg agtttgacgt catcgccgcg
     3361 gtcgtggtcg gcggtacggc actttccggc ggtcgcggtt ccctgttcgg tacattgctt
     3421 ggtgtgctgg tgattacgct aatcggtaac ggtctggtgc tgctcggtat taactccttt
     3481 ttccagcagg tggtacgcgg cgtcatcatc gtggtggcgg tgctggcgaa tatcttgctg
     3541 acccagcgaa gcagtaaagc gaaacgctaa accttaacgc ccctggcgcg ggtgcggcag
     3601 ggatgaaaat taatcgtatt gttaatgagg gatttatgaa aacgatgctg gcagcttatt
     3661 taccaggaaa ttcaaccgtc gatctgcggg aagttgcggt gccgacgccg gggattaacc
     3721 aggtactgat caaaatgaaa tcctccggga tttgcggaag cgatgtccac tatatctatc
     3781 atcaacaccg tgccacggcg gcggcacccg ataaaccgtt ataccaggga tttatcaacg
     3841 gtcatgaacc atgcgggcag attgtggcga tggggcaagg ctgtcgccat tttaaagagg
     3901 gtgatcgcgt gctggtgtat cacatttctg gctgtggttt ttgcccgaac tgccgccgcg
     3961 gctttcctat ctcttgtact ggcaaaggaa aagcggctta cggctggcag cgtgacggcg
     4021 gtcatgccga atacttgctg gcggaagaaa aagatctgat cctcctgccg gatgcgctta
     4081 gctacgaaga tggtgcgttt atcagttgcg gcgttggtac agcgtatgaa ggaattttgc
     4141 gcggcgaagt ttccggcagt gataacgtgc tggtggtcgg tctggggcca gtcggcatga
     4201 tggcgatgat gctggcgaaa ggtcgcggtg caaaacggat catcggcgtt gatatgctgc
     4261 cggaacgtct ggcgatggca aaacagttag gggtgatgga tcacggctat ttagcaacca
     4321 ccgaaggtct gccgcagatt atcgccgaac tcacccacgg tggcgcggat gttgcgctcg
     4381 attgttctgg taatgccgca ggtcgcttgc tggcactgcg atccaccgct gattggggac
     4441 gggtggttta catcggagaa accggaaaag tggagttcga ggtcagcgcc gatctgatgc
     4501 accatcaacg gcggattatc ggctcctggg tgaccagtct gttccatatg gaaaaatgcg
     4561 cccatgatct gacggactgg aaactgtggc cgcgtaacgc cattacccat cgcttctcgc
     4621 tggaacaggc tggagatgcc tatgcgctga tggcgagcgg caaatgcggg aaagttgtga
     4681 ttaacttccc ggattaagga gttagcggat gaccatctat accttatctc acggaccgct
     4741 aaagctggat gtctctgacc agggcggcgt aatcgaaggc ttctggcgtg acacgacgcc
     4801 gcttttacgt cctggtaaaa aaagcggtgt ggcaaccgat gcttcctgtt ttccgctggt
     4861 gccgtttgca aaccgggtga gcggcaatcg ttttgtctgg caggggcgtg agtatcaact
     4921 gcaaccgaat gttgagtggg atgcgcacta tctacacggc gatggctggc tgggcgagtg
     4981 gcaatgtgtc tcgcacagcg atgatagctt gtgtctggtg tatgaacatc gcagcggtgt
     5041 ctatcactat cgggtaagtc aggcgtttca tttaacggcg gatacgctga cggtgacgct
     5101 ctctgtcacc aatcaagggg cagagacgct gccatttggt accggctggc atccttattt
     5161 tccgttgtca ccacaaacgc ggattcaggc gcaggcgagc ggttactggc tggagcggga
     5221 gcagtggctg gcgggtgagt tttgcgagca gctaccgcag gaactggatt ttaaccagct
     5281 cgcgccgttg ccgcgccagt gggtgaacaa tggttttgcc ggatggaatg gtcaggcccg
     5341 catcgagcag ccgcaggaag gttatgccat catcatggaa acgacgccac ctgcaccgtg
     5401 ttatttcatc tttgtttccg accctgcgtt tgataaagga tatgcgtttg atttcttctg
     5461 tctggaaccg atgagccatg cgccggatga tcatcatcgc ccggaagggg gtgatctcat
     5521 tgcgctagca ccaggggaat caacaacttc agagatgtcg ttgcgggtag agtggctgta
     5581 atttattaaa tgccggataa ggcgttcacg ccgcatccgg catctttcct aacgccgatc
     5641 gagagaaatt gcccccggcc cggtaattgc caatagcaag aacgcgccag cgatactgac
     5701 attcttccag aaattaatca tatttggccc aaccgcatcg ccggtcatat cccagtaatg
     5761 gtgaccaatc accgccgtac ccagcgtgta gaaaataaac agcaccgcca gcggacgggt
     5821 gaaaaagcca agcacgatta atatcgcggc gggcacttcc ataactaccg caataatcgc
     5881 tgccagcatc ggcattggcg cgcccaacga ggccatatat tggaccgtac cgtcaaagcc
     5941 catcattttg ggaaaaccca aaataatgaa aattaagacc acggcgatac gggcaattaa
     6001 taacaaaacg gggcgggcag ctccaaaatc aaaataacgt agtgtgttca taaccaatcc
     6061 ctttgcaaga taatgtgatt taaacgtaat acacactctt gcacctcgcc acgccccagg
     6121 cgttgagata ttcgctaatt atcctggtta cagtgattta agcgcgatgt tctcatctat
     6181 cagcagtttc gcatcaaacg ttttgccgct ctggatccat ttgcgaattg ggcgaatctc
     6241 acgcccctga ttcagcgtga ccgcaccgat aagcacgccg ttttgcagat taaaccaaat
     6301 cgccttctga gtttccgggt tgccacgaca aagccag
//
LOCUS       DAZPEB010000203         6330 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZPEB010000203 DAZPEB010000000
VERSION     DAZPEB010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6330
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.208"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            239..1699
                     /locus_tag="ACHURJ_003614"
     CDS             239..1699
                     /locus_tag="ACHURJ_003614"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFR1282402.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYKVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAKAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(1735..1938)
                     /gene="ydfZ"
                     /locus_tag="ACHURJ_003615"
     CDS             complement(1735..1938)
                     /gene="ydfZ"
                     /locus_tag="ACHURJ_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFR1282403.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(2116..2801)
                     /gene="rspR"
                     /locus_tag="ACHURJ_003616"
                     /gene_synonym="ydfH"
                     /pseudo
     CDS             complement(2116..2801)
                     /gene="rspR"
                     /locus_tag="ACHURJ_003616"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
     gene            complement(2890..3636)
                     /gene="ydfG"
                     /locus_tag="ACHURJ_003617"
     CDS             complement(2890..3636)
                     /gene="ydfG"
                     /locus_tag="ACHURJ_003617"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFR1282404.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3773..5818
                     /gene="dcp"
                     /locus_tag="ACHURJ_003618"
     CDS             3773..5818
                     /gene="dcp"
                     /locus_tag="ACHURJ_003618"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFR1282405.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(5863..>6330)
                     /locus_tag="ACHURJ_003619"
     CDS             complement(5863..>6330)
                     /locus_tag="ACHURJ_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFR1282406.1"
                     /translation="ENLTSALSQRLIADQLRLTTAESCTGGKLASALCAAEDTPKFYG
                     AGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAIERADADVSIAITGYGGL
                     EGGEDGTPAGTVWFAWHIKGQTYTAVMHFAGDCETVLALAVRFAHVQLLQLLL"
BASE COUNT         1583 a         1512 c         1628 g         1607 t
ORIGIN      
        1 gccattatga tcatggcata ttcagtgatt ggtttaatct ctgcgctgaa aatgccagag
       61 gtgaaagacc gtgatttaag tgcgctggaa gacgctgcgg aagattaacc gcgtgttgta
      121 agagctgcgc aaccttccag aagtctgtaa accctcaatc ccttctctta ccggagaggg
      181 gatttttatt catataacaa aacatatagc ttgccatatt taaatttaag gaattgttat
      241 gggaaataat ttgttatcag caaaagcgac actccctgtt tatgatcgta ataacctggc
      301 cccaagaatt gttcatttag gctttggtgc atttcaccgt gcgcatcagg gcgtgtatgc
      361 cgatattctt gctacagaac atttcagtga ctggggatat tataaggtca acttaatcgg
      421 cggcgaacag caaattgccg atttacaaca gcaagataat ctttataccg ttgcggaaat
      481 gtcggccgat gcgtggacgg ctcgcgtcgt tggcgtcgtt aaaaaagcct tgcacgtaca
      541 gatggatggc ttagaaaccg tgttggctgc gatgtgtgaa ccgcaaatcg cgattgtctc
      601 tctgacaatc accgaaaaag ggtatttcca ctctccggcg accggacagt taatgctcga
      661 tcacccgatg gtcgctgccg acgtgcaaaa tccccaccag ccgaaaacag caacaggggt
      721 gattgttgag gcgctggccc gccgtaaagc ggcaggactt cccgcattta ccgtcatgtc
      781 atgtgacaac atgccagaaa acggtcatgt tatgcgtgac gttgtcactt catacgcgaa
      841 agctgttgat gtaaaactgg cacaatggat cgaagataac gtgactttcc catcaacaat
      901 ggtggaccgt attgtgcccg cagtgacaga ggatacgctg gcgaaaatcg aacaacttac
      961 cggtgtgcgc gatcctgcgg gcgttgcctg tgaacctttc cgccagtggg taatagaaga
     1021 taactttgtt gccggacgtc cagaatggga aaaagcggga gccgaactgg ttagtgatgt
     1081 gctgccttat gaagagatga agttgcgcat gctcaacggc agtcattcat tcctggcgta
     1141 tttgggttat cttgccggat atcagcacat taatgactgc atggaagatg aacattatcg
     1201 ttatgcggcg tatggcttga tgttgcagga acaagcgccg acgctgaaag tgcagggcgt
     1261 tgatttgcaa gattacgcta accgattaat tgaacgctat agcaatccgg cgttacgtca
     1321 tcgaacctgg cagattgcga tggatggtag ccagaaattg ccacagcgga tgttggattc
     1381 tgttcgctgg catctggcgc atgacagcaa gttcgatctg ctggcgctgg gcgtcgcggg
     1441 ttggatgcgt tatgtcggtg gtgttgatga acagggaaat ccgatagaaa tcagtgaccc
     1501 actgttacct gttattcaga aggctgtaca aagtagtgcc gaagggaaag cgcgcgtcca
     1561 gtcattgctg gcgattaagg caatctttgg tgatgatttg ccagacaata gcttgtttac
     1621 tgcaaaagtg acggaagcgt acttgtcttt attagcgcat ggcgcgaaag cgaccgtggc
     1681 gaaatattcc gtgaagtaaa aaaagcaatg ccgccatcgg cggcattcac acacttagtt
     1741 catgccgaga cgaatcaggt ccagaggtgc cagtttctct tcacaacctt caacaactac
     1801 ggtttttccg cggcggattt gctcagcggt cagaccttca gtatcaatcg acaggatctt
     1861 gccagtgtga ccggtgccgc taaccataac acggctgcca gtggtgattg cgttacggtt
     1921 acgatcgtag gtcgtcatag gtgttttctc ctttctgatt tacagtcgaa gcccacgtaa
     1981 cgggcgcaat ataaatactc ctgtcgagtg aaatattttt tgtttttgat caagctcaca
     2041 tcttttttga tgcatgcatg agccgtaagg cggggggaaa agcctctcat cccggatggg
     2101 atgagagtaa atgaattact cttcgctaaa ccagtcgctg ttttcctggc ggatctggcc
     2161 acggattcgc tgatttcctg caaatgttgc gtcattgcac gttctaccgc atcgccatca
     2221 cgtttttgca gggcagagaa aatatcaaga tgctggcgta acagcatttc tggtggagaa
     2281 acatggtcga aactcatata gcgcacgcga tcaacggtcg ctttcaggtt ctcaatggta
     2341 tcccacgcca gttgacagtc ggcaatctgc gtcaggagtt gatggaagtt gtcatcaagt
     2401 tcaaaaaaat catccagttg cttgtgctca atggcaatgc gttgctggtg aagattttgt
     2461 tccagttggt agcactggct ttcggtaatc atgctcgccg cccgacgcgc caccgcgcac
     2521 tcaatggcct gacggataaa actgccgttg cgcacctggg ccatggaaat tttgttgacg
     2581 tagctgccac gctgtggacg aatttgaatc aggccgtttt ccgccagttt aataaaggct
     2641 tcacgaaccg gctggcgtga cacattgaaa cgaacagaaa cttctttttc cgacaacggt
     2701 gtgcctggag caatcaggca atggacaatg tcgcgacgaa ggatacgata aatttgctga
     2761 ttgacaggct gtgtgggatt aagttgcgtt tcgacggtca ttcgttcttg cttgtgagtg
     2821 agttaactgc atgagtctac cacttttttg tgacaagcaa taacccggca gttacgccgg
     2881 gtataaaaat tactgacggt ggacattcag tccggcatag ctttgggtaa ccggcatcat
     2941 ttccagggta ttgatattga cgtgagcagg cagcgttgac acccaccaaa cggcttcgct
     3001 gacatcttct ggcgtcaatg caacggtatt ttgataggtt ttttccgctt taccgtcatc
     3061 gcctttaaag cggacattgg aaaactcggt gccacccacc agacccggtt cgatgtcggt
     3121 gacgcgcacc gccgtaccat gcaggtccgt acgcagattc aggctaaact gacgaacaaa
     3181 cgctttcgtc gcaccgtaaa cgttaccacc ggcataaggc cagctacctg ccgttgagcc
     3241 aatgttaata atatgaccat gattacgttc aaccataccc ggtaagacgg cgcgcgtcat
     3301 atataccagg cctttgttgt tggtatcaat catcgtttcc cagtcttcaa cgctggcttt
     3361 atgcgcaggc tccatgccca acgccaggcc ggcattattt accaggatat caatattgca
     3421 ccactcggca ggaagcgatg ccagcatctc ttcaatagcg gcgcggttgc gaacgtccag
     3481 ttgggcgatg tacagattat ctcccagttc gtcttttaac tcctgcaacc gttcctggcg
     3541 acggccagtg gcgataactt tatgcccttg ttgaataaaa cgacgagtaa tgcattcacc
     3601 aaaacctgcc gttgctccag ttactaaaac gatcatctca ctgttcctca acgcttttgt
     3661 gtgtcaataa catagcacgt gatgcttaag agcgggtaat actggtttgt aagattgcac
     3721 tccgttatca gtaagcctac gctatggaac acctcgcgtt caggagaaga aaatgacaac
     3781 aatgaatcct ttccttgtgc aaagcacact accgtatctg gctccccatt ttgatcaaat
     3841 tgccaatcat cactatcgcc cggcattcga tgagggaatg cagcaaaagc gggcagaaat
     3901 tgctgccatc gcgcttaacc cgcaaacgcc tgatttcaac aatactattc tggcactgga
     3961 acaaagcgga gaattactta cccgcgttac cagcgtcttt tttgcgatga ctgcggcgca
     4021 taccaatgat gaattacagc gtcttgatga acagttttcc gctgaactgg cggaactggc
     4081 taatgatatc tatctgaacg gtgaattatt cgcgcgggta gatgctgtct ggcagcgccg
     4141 tgaatccctg gggcttgata gtgaatccat ccgcctggtg gaggtgattc atcaacgttt
     4201 tgtccttgcc ggagccaaac ttgcgcaagc tgataaagca aaattaaaag tactgaatac
     4261 agaagctgcg accctgacca gccagtttaa ccagcgatta ctggcagcaa ataaatccgg
     4321 cggtctggtt gtgaacgata tcgcgcagct ggcaggaatg agtgagcaag agattgcgct
     4381 ggcggcagag gcggctcgcg agaaaggtct ggataacaaa tggctgattc cgctgctgaa
     4441 taccacccaa caaccggcgc ttgccgagat gcgcgatcgt gcgacgcgtg aaaaactgtt
     4501 tattgcgggc tggacgcgag cggaaaaaaa tgatgccaat gatacccgcg ctatcattca
     4561 acgtctggta gagattcgcg cacagcaggc gaaactgctt ggttttcctc attatgccgc
     4621 atggaaaatc gccgatcaga tggcaaaaac gccagaagca gcactcaact ttatgcggga
     4681 aattgttcca gcggcgcgtc aacgtgctag cgatgaatta gcctccatac aggcggttat
     4741 cgataagcag caaggcgggt ttagcgcgca gccgtgggac tgggcatttt atgccgaaca
     4801 ggtacggcgg gagaaatttg atcttgatga ggcgcagctc aagccatatt ttgaattaaa
     4861 cacggtgttg aatgaaggtg tattctggac cgcgaatcag ctcttcggta ttaagtttgt
     4921 cgaacgtttt gatattcctg tctaccatcc tgacgttcgt gtgtgggaaa tttttgatca
     4981 taatggcgtg ggactggcgt tattttacgg tgatttcttc gcccgtgatt caaaaagcgg
     5041 cggtgcatgg atgggcaatt ttgttgagca atcaacgctt aatgaaacgc atccggtaat
     5101 ttataacgtt tgcaattatc agaaacccgc tgccggtgag cctgcgttgt tactctggga
     5161 tgatgtcata accttattcc atgaatttgg tcatacgctg cacggccttt ttgcccgcca
     5221 gcgttatgcc acgctttccg gcaccaacac gccgcgtgat tttgtcgaat ttccgtcgca
     5281 aatcaacgaa cactgggcaa cgcatccgca ggtattcgct cgctacgccc ggcattatca
     5341 gagcggggca gcaatgcctg acgaactgca acagaaaatg cgtaatgcca gcctgttcaa
     5401 caaagggtat gagatgagcg aactgcttag cgccgcactt ctcgatatgc gctggcattg
     5461 cctggaagaa aacgaagcaa tgcaggatgt cgatgatttt gaattgcggg cgctggtggc
     5521 ggaaaatatg gatcttcctg ctataccgcc acgctatcgc agcagttatt tcgcccatat
     5581 ttttggtggc ggatatgctg caggttatta cgcttatctg tggacgcaaa tgttggccga
     5641 tgatgggtac cagtggtttg ttgagcaggg cggattaacg cgtgaaaatg ggcagcgttt
     5701 tcgcgaggcg atcctttcca gaggtaacag cgaagatctg gaacgcctgt atcgacaatg
     5761 gcgcggtaag gctcctcaga ttatgccgat gctgcaacat cgtggcttga atatataaac
     5821 accaccaatg gctggcctgg agatatcgcc aggccagcct ggttatagca gtaattgcag
     5881 cagctggacg tgggcaaacc tcaccgctaa agctaatacc gtttcgcagt cgccagcaaa
     5941 atgcataacc gcagtgtagg tctggccttt aatatgccac gcgaaccaga cggtacccgc
     6001 tggcgtacca tcttcaccgc cctccagtcc gccgtagccg gtaatggcaa tactgacatc
     6061 agcatccgca cgctctatgg cacctgttgc catttctgct gccactttct cactcaccgc
     6121 agaatatcgt tcaagagatt gctggcttac gctgaggatt ttcatctttg cctgatcggt
     6181 gaaagtaaca aagcctgcac cgtaaaattt gggtgtatct tcagctgcac acagggcgct
     6241 agccaactta ccgccggtgc atgattcggc ggtagttaag cgtaattgat ccgcgataag
     6301 tctttgactc aacgcggatg tcaggttttc
//
LOCUS       DAZPEB010000204         6219 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZPEB010000204 DAZPEB010000000
VERSION     DAZPEB010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6219
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.209"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..1471
                     /locus_tag="ACHURJ_003620"
     CDS             <1..1471
                     /locus_tag="ACHURJ_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282407.1"
                     /translation="VLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEGKTC
                     RQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDAFHA
                     RASHYHAGECLRQLAALNSRLNCAQEMARRDSVGEVPPVPWRTVVGSGIAGEAKLDHL
                     RLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSFQAG
                     ALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALREYL
                     RQRPPACIRPLNQVDNLFILPVAECISLGWDSGRQTLDAQVISGEGEDNLLTLSLPAS
                     ACSPFAVERMAALLQQTDDPVSLVSGFVSFVEGQLTLEPRVMMTKTRAWALDAETAPV
                     APLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLTAVGFY
                     RLAHVLVQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            1596..2057
                     /locus_tag="ACHURJ_003621"
     CDS             1596..2057
                     /locus_tag="ACHURJ_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFR1282408.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASISATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(2098..2568)
                     /locus_tag="ACHURJ_003622"
     CDS             complement(2098..2568)
                     /locus_tag="ACHURJ_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFR1282409.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(2615..3334)
                     /gene="btsR"
                     /locus_tag="ACHURJ_003623"
                     /gene_synonym="yehT"
     CDS             complement(2615..3334)
                     /gene="btsR"
                     /locus_tag="ACHURJ_003623"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFR1282410.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(3331..5016)
                     /gene="btsS"
                     /locus_tag="ACHURJ_003624"
     CDS             complement(3331..5016)
                     /gene="btsS"
                     /locus_tag="ACHURJ_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFR1282411.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLQLPWRDEA"
     gene            5238..5303
                     /locus_tag="ACHURJ_003625"
                     /pseudo
     CDS             5238..5303
                     /locus_tag="ACHURJ_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5531..5779
                     /locus_tag="ACHURJ_003626"
     CDS             5531..5779
                     /locus_tag="ACHURJ_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFR1282412.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(5895..6164)
                     /locus_tag="ACHURJ_003627"
     CDS             complement(5895..6164)
                     /locus_tag="ACHURJ_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR1282413.1"
                     /translation="MLLQHSDIEIAWYASIQQEPNGWKTVTTQFYIQEFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNCVDTSTAPDIEWPEEPDTM"
BASE COUNT         1602 a         1589 c         1481 g         1547 t
ORIGIN      
        1 cgtactggca gtacaggcct ttgtccaggc aaaagcgcag caagcggaat tgactcattt
       61 aatctggcag atgcgcagcg aacacgtcac atcatctgac gatccgtttg ccagcgaaga
      121 aggcaaaacg tgtcgtcaat atgttcaaca actaagccag gcattatggc tgagcggcat
      181 cagccagccg cttattcact acgaggccgc tttcagccgc gcgcagcaag cagcggaacg
      241 ctgcagctgg cgatgggtga gtgaatcgct acggcaactg cgcgccagcg ttgatgcctt
      301 ccacgcccgc gccagccact atcatgccgg agaatgctta cgtcagcttg cggcattaaa
      361 cagtcgatta aactgcgcac aagagatggc ccggcgcgac agtgttggtg aagttcctcc
      421 tgtgccgtgg cgcacggtcg ttggctctgg cattgccgga gaagcaaagc ttgatcatct
      481 gcggctggtg tctttaggta tgcgttgctg gcaggatatt gagcattatg gtttacgcat
      541 ctggtttacc gatcccgaca ccggcagtat tttgcatctt tcgcgtagtt ggccgcgaag
      601 tgaacaggaa aactcaccgg cagctacgcg tcggctgttt agttttcagg ctggcgcact
      661 ggcgggtggg caaattgttt cacaagcagc aaaacgcagt gccgatggcg agctgctgtt
      721 agctacccgc aaccgcttaa gcagcgttgt gccgctgtcg ccagacgcct ggcaaatgtt
      781 gagcgcgccg ttacgccagc cgggcattgt ggctttgcgg gaatatttac gccagcgtcc
      841 ccccgcctgc atacggcctc ttaatcaggt ggataactta tttattctgc cggtcgctga
      901 gtgtatttca ctcggttggg acagcggccg ccagacgctg gatgcgcagg taatcagcgg
      961 cgaaggtgaa gataatctgc tgacgttatc ccttccggca tctgcctgtt ctccttttgc
     1021 cgttgaacgc atggcggcgc ttttgcaaca aacagacgac cccgtgagtc tggtttctgg
     1081 ctttgtcagc tttgtagagg ggcaattgac actggaacca cgggtgatga tgacaaaaac
     1141 ccgtgcctgg gcgctggacg cggaaactgc gcctgtggca ccgctacctt ctgccagcgt
     1201 tttgcctgtg ccgtctaccg ctcatcagtt gctgatgcgc tgccaggcgt tacttattca
     1261 actgctccat aacggctggc gctatcagga acagagcgct attagtcagg cagagttgct
     1321 ggcgaatgac ctcaccgcgg tgggttttta tcggctggca catgtgttgg tacaatttcg
     1381 taatacagaa agcgaggcac gggtagaagc aatgaataac ggtgttttgc tttgcgaaca
     1441 attattcccc atgcttcagc aacaaggatg aaatagtgct ttttactaag agttctactc
     1501 cagttccgga ctgctcacgc cacggtatta ggcatatcct atatagcccc tggtgagagt
     1561 caccagtttc ttgattaaat aaaatggagt tttacatgaa ggctttcaat aagctgtttt
     1621 ccctcgttgt tgcatctgtt ctggttttct ctcttgctgg ctgcggtgac aaagaagaat
     1681 cgaagaaatt cagcgccaat ctgaacggca ctgaaattgc cattacctat gtctacaaag
     1741 gtgacaaggt gcttaagcaa tcttctgaaa ccaaaattca atttgcctcc attagtgcaa
     1801 ccaccaaaga agatgctgcc aagacacttg agccgttaag cgccaaatac aaaaacatcg
     1861 cgggtgttga agaaaaatta acctataccg atacctacgc gcaggaaaac gtgactatcg
     1921 atatggaaaa agtggatttt aaagccctgc agggtatttc aggaatcaac gtttctgctg
     1981 aagatgccaa aaaaggtatc actatggcgc aaatggaact ggtgatgaaa gccgctggtt
     2041 ttaaagaagt gaaataatct gtcggcggcc atgtttcgta tggccgccat acccgtctta
     2101 gcttttcttc acatgctggc gcgctgccag tccacgcaga aaataacgta aaaattgatc
     2161 gccgcattcg cggaagtttt tatgatccgg tgcgcgcatc atcgctgtaa tttccggcat
     2221 cgaaacgcgg aactgctgtt cggtgaggat agccagaatg tcatcggttt tcagcgaaaa
     2281 cgcgatgcgt aattttttca gcacgatgtt gttattaatg cgacgttccg gctccagtgc
     2341 cggagcagac tcatccttgc cgcgtttttc ataaatcagg ccattgagga atgacgacaa
     2401 aacaatgtcc ggacaacgct gaaaaccctc ttcgtcttct ttacgtagcc agacggcgat
     2461 ctgttccgcg gtggcttcga cattacccag cgccagaata cgcaccaggt cattattatt
     2521 ggctttcaaa atgtagcgca cgctgcgcag aatatcgtta cttagcatga ggccttcagg
     2581 tgttgatgag gcaaaaagcc attttagcag tcttttacag gccaatcgcc tcttttaagc
     2641 ttttcagata acggcggctg accggcacgg ttaagccatt acgcaaaatc aactcggcct
     2701 ggccgttatc ttccagacga atctcctgta aatgcgcgag gttaaccaga tactgacgat
     2761 ggcagcgcag tagtggtgta cgactttcca gggtacgtaa tgtcaattcg gtaaagccct
     2821 ctttcccttc gtggctggta acgtagacac cgctcatccg actgctgaca aatgccacat
     2881 ctttcatttg cagcaaataa atccgactat gccccgtaca agggataaat ttcagcgcct
     2941 gttgattttc cggtaacagc gaaacatcct gcttgctgcg ctcctgacgc aatcgcgcca
     3001 gcgttttctc cagtcgcgct tcatcaattg gcttcagcag ataatcaaag gcatgttctt
     3061 caaaggcttt aatagcgtat tcgtcaaacg cagtgagaaa aacaatatac gggcgatgtt
     3121 ccgggtcaag catccccacc atttccagac cactgatgcg cggcatctgg atatcgagaa
     3181 acagcacatc cgggcgcagt ttatgcaccg cgccgatccc ttccacggcg tttgaacact
     3241 ctccaacgat ttcaatatcg ctctgctcct gcaaaaatac acgcaggttc tcccgtgcta
     3301 acggttcatc atcgacaatt aagactttaa tcatgcctcg tccctccatg gtagttgtaa
     3361 cgttattcgg gtgtaactat caggctcaca ggcgacgctt attccatagt catcgccaaa
     3421 ccgttcacgt aaacgcttat ccaccagatt catccccagc ccactggcat tggttaccgg
     3481 ttgatacaaa ccggcattgt cttcgatctc cagcatcaaa tgttgcccct cacgtcgggc
     3541 gctgattgcc actcgccctg tatccagcag ttgtgatgtc ccatgtttaa tggcgttttc
     3601 cactatcggt tgcagggtaa acgcgggcaa ttgctgctgg gataattctt gcggaatagc
     3661 aatgttgacc tgcaaccgcg actggaagcg cgccttttca atttgcagat aagcgttcac
     3721 atgttcaatt tcgtcggcga gagtaacaaa ctccgaaggc cgctttaagt ttttgcggaa
     3781 aaaagtggaa agatactgca ccagctggct ggcctgttcg ctgtcgcggc ggatcaccgc
     3841 tttaatggtg ttaagcgcat taaacaaaaa atgaggattc acctgggcgt gaagcagttt
     3901 gatttctgac tgggtgagca tcgctttttg ccgctcatat tgcccggcaa gaatctgcgc
     3961 cgaaagcagt tgcgcaatcc cctcgcccag cgtgcggttt attgaactga ataaacggtt
     4021 tttggcttca tacaatttga tggtgcccat cacccgctga ttttcaccac gcaacggaat
     4081 taccagcgtc gaccccagtt tgcattgcgg atgcaaagag caacgataag gtacttcgtt
     4141 gccatcagcg tagaccactt caccggtttc aatcgctttt aaggtgtaag tcgaagaaat
     4201 cggtttgccg ggtaaatggt ggtcgtcacc aattccagta aaggccagca atttctctcg
     4261 atcggtaatc gcgactgcac caatatccag ctcctgatac agcacctgtg ccactttcat
     4321 gctgttcact tcgttaaacc cctggcgcaa aatgccttcc gtcgaggcag ccactttcag
     4381 cgcagtggca gaaaaagccg aagtgtattt ttcaaacatc gcgcgtttat cgagcaatat
     4441 acgcataaac agcgccgcgc cgacggtatt ggtgaccatc attggtgcag caatattact
     4501 caccagacgc accgcatctt cataaggtcg ggcgatcgcc aggatgatca acatttgcac
     4561 catttcagcg acgaacgtga cggcaccggc ggtaatgggg ttaaagactt tatcagtacg
     4621 cccgcgacga atcaggatgc tgtgtaccag gccgccgagc aatccttcaa cgatggtcga
     4681 gatcatgcaa cttagcgcgg tcatgccccc catcgaatat cgatgtaacc cgccggtcag
     4741 gccaaccagc ccaccgacga ccggaccgcc gagtaagccg cccattaccg cgcctatcgc
     4801 acgggtattg gcaatagaat cgtcaatgtg caacccaaac caggtgccca tgatgcagaa
     4861 gatggaaaag acgatgtagc agagaaattt atgcggcaga cgaaccgtga cctgcattaa
     4921 cggtatgaat aatggcgttt tactcattaa ccacgcaatg actaaaaaaa cgcacatctg
     4981 ctgaagcagc agcaacacca gattaaaatc gtacataccc gcaaaccaca cttcccttta
     5041 aaacgcgtaa catacattgc ctgcgtttaa ctttctttga actcttgcag aaaaatgaga
     5101 attcgtgagt acgatcactc aaaatcgcct ggcaaaaata aaatcaccct atagatgcac
     5161 aaaaaacggg caaaactacc tggttcgcaa aactgcgtct aaagttaaac cgggacctcg
     5221 cgagcaaggg tgagacgatg gcgctttaca caattggtga agtggcgttg ctttgtgata
     5281 ttaaccctgt cacgttacgc gcgtgacgca acattttaaa aaatcgacga agatcgtcca
     5341 ggagcgccgt ttttcaaggg ttggatagat gattcgttta ccatgtcgac gaacttcgac
     5401 gaatttcgcc gataaaaagt gtcccataca tgccccattg atgccccata aacaatatca
     5461 gaaattgata ctggttatcc atacagataa aaataacacc tgtacataac tacagttaca
     5521 ggagggtgtt atgcgtgttg agatctgtat cgctaaagaa aaaatcacta aaatgccaaa
     5581 cggtgctgtg gatgcgttaa aggaagaatt aacccgacgc atcagtaaac gttatgacga
     5641 tgtagaggtg atcgtaaaag ccaccagcaa cgatggcctt tctgttacac gcaccgcaga
     5701 taaggattct gcaaaaacat ttgttcagga gactctgaaa gatacctggg agtctgctga
     5761 cgagtggttt gttcactaat aaacacgtaa aatcggtaac ggctggaaat cattcaatac
     5821 tcgcactatc gaaagtttac cagccaaccg cagcacgtcc tgcatacgtc gtgtctgcgg
     5881 tttttctttt tcgcttacat tgtgtctggt tcttccggcc actcaatatc aggtgcagtt
     5941 gatgtatcaa cacagttcag caacacccga tacttcttcc aggcttccag caacgagttt
     6001 tcttcctccg ttgcgatctc cagatctaca gcatcctgaa gtggcgcaat atgctcactg
     6061 aattcctgga tgtagaactg tgtggtgacg gtcttccagc cattcggctc ctgctgtatc
     6121 gaagcatacc aggctatttc aatatcgcta tgctgcagca gcatttaacc ccttgtaatt
     6181 catcgccata attgatttaa ttcacaaata aaactataa
//
LOCUS       DAZPEB010000205         6145 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZPEB010000205 DAZPEB010000000
VERSION     DAZPEB010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6145
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.210"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            161..937
                     /gene="exuR"
                     /locus_tag="ACHURJ_003628"
     CDS             161..937
                     /gene="exuR"
                     /locus_tag="ACHURJ_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFR1282414.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1177..1260
                     /gene="yqiM"
                     /locus_tag="ACHURJ_003629"
     CDS             1177..1260
                     /gene="yqiM"
                     /locus_tag="ACHURJ_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFR1282415.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            1282..1944
                     /gene="yqjA"
                     /locus_tag="ACHURJ_003630"
     CDS             1282..1944
                     /gene="yqjA"
                     /locus_tag="ACHURJ_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFR1282416.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1948..2331
                     /gene="mzrA"
                     /locus_tag="ACHURJ_003631"
     CDS             1948..2331
                     /gene="mzrA"
                     /locus_tag="ACHURJ_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFR1282417.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2478..2846
                     /gene="yqjC"
                     /locus_tag="ACHURJ_003632"
     CDS             2478..2846
                     /gene="yqjC"
                     /locus_tag="ACHURJ_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFR1282418.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            2884..3189
                     /locus_tag="ACHURJ_003633"
     CDS             2884..3189
                     /locus_tag="ACHURJ_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFR1282419.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3192..3596
                     /locus_tag="ACHURJ_003634"
     CDS             3192..3596
                     /locus_tag="ACHURJ_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFR1282420.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3586..3885
                     /locus_tag="ACHURJ_003635"
     CDS             3586..3885
                     /locus_tag="ACHURJ_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFR1282421.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            4071..4463
                     /locus_tag="ACHURJ_003636"
     CDS             4071..4463
                     /locus_tag="ACHURJ_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFR1282422.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSKFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4533..5436
                     /locus_tag="ACHURJ_003637"
                     /pseudo
     CDS             4533..5436
                     /locus_tag="ACHURJ_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004364 - glutathione transferase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
     gene            5729..6094
                     /locus_tag="ACHURJ_003638"
     CDS             5729..6094
                     /locus_tag="ACHURJ_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFR1282423.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
BASE COUNT         1546 a         1503 c         1541 g         1555 t
ORIGIN      
        1 agctttcaca atgacgatac ttatttttta ggaatttata aatattcaca atattaattc
       61 aatacattaa ttcaatcacc aaatttatat atgaccaatg gcatacccat gaataaagtg
      121 gtatgataac ttcgtccgca ttccactccg ggagacgcat atggaaatca ctgaaccacg
      181 ccgtttgtat caacaacttg ccgctgacct gaaagagcgc attgaacagg gcgtctatct
      241 ggtgggtgat aaactgcctg ccgaacgctt tattgccgat gaaaagaacg tcagccgaac
      301 ggtagttcgt gaagccatca tcatgctgga agttgaaggc tatgtggagg tgcgtaaagg
      361 ttcaggtatt catgtggttt ccaaccagcc gcgccatcag caggcggctg acaataatat
      421 ggaattcgcc aattacggtc cgtttgagtt gcttcaggct cgccagctca tcgaaagtaa
      481 tattgccgag ttcgcggcaa ctcaggtaac gaaacaagac atcatgaaac tgatggccat
      541 tcaggaacag gcgcgcggcg aacaatgctt ccgtgattcc gagtgggatt tacagttcca
      601 cattcaggta gccctggcga cgcagaactc cgccctggcg gccatcgttg aaaaaatgtg
      661 gacccagcgt agccataacc cgtactggaa aaaactacac gaacacattg attcccgtac
      721 cgttgataac tggtgtgatg accacgatca aattctcaag gcgttaattc gcaaagatcc
      781 ccatgctgct aagctggcaa tgtggcagca tctggaaaac accaaaatca tgttatttaa
      841 cgaaaccagc gacgatttcg agttcaatgc cgaccgctat ctattcgccg aaaacccggt
      901 agtgcatctc gatacggcca ccagtggcag taaatgaaac tcctgtccga caggcgctgc
      961 gacgcgcctg ttcgcttcgt cggtaagcga aaagtataaa gtgtcagcct gtgtaaatcc
     1021 tctcgccacc ctcccctgca ttcagcaaaa tcagactcca cggacatgta attttgataa
     1081 cgaacaacgt tgacctttgt tacaattaga ttcaatttga atttatgttt ttgaatgctt
     1141 tcttatctca cgatttaaca gggaatagtt caggctgtgt tgatgtatca aacccgcaga
     1201 acataccaaa acagcaataa cattgcggta gtgcatcttt taaaaccagc gtggcgttaa
     1261 ccgattcacc aggaataatg aatggaactt ttgacccaat tgctgcaagc cctgtgggcg
     1321 caggattttg aaaccctggc caatccatcg atgattggca tgttgtattt tgtcttgttt
     1381 gtaattttgt tccttgaaaa cggcttgctt ccagcggcct ttttaccggg cgacagttta
     1441 ctggtattgg tcggcgtgtt gattgcgaaa ggcgcgatgg gttacccgca aacgattctg
     1501 ctgctgaccg ttgccgccag cctcggctgc tgggtcagct atattcaggg gcgatggctg
     1561 ggcaataccc gcaccgtaca aaactggcta tctcatttac ccgcgcatta tcatcaacgc
     1621 gcacaccatc tttttcataa acacggttta tcggcgctgt taattggtcg ctttattgcg
     1681 tttgtcagaa cactgctgcc gacgattgcc gggttatcag ggctgaataa cgcgcgcttt
     1741 cagtttttca actggatgag cggtctgctg tgggtattga tcctgacaac tctgggttac
     1801 atgctcggca aaacgccggt atttttaaag tacgaggacc agctgatgtc atgcctgatg
     1861 ctgctcccgg tggtgctgct ggtgtttggc ctggcaggtt ctctggtcgt gttatggaaa
     1921 aagaaatatg gaaatcgggg gtaagggatg caaatacctc gcatgtcgct tcgccagcta
     1981 gcctggtccg gcactgtttt acttctggtc ggcacgctgt tgctggcctg gtccgcggtt
     2041 cgccagcaag agtctacgct ggcgattcgt gccgttcatc aaggcacaac gatgccagac
     2101 ggtttttcga tctggcatca ccttgacgct catggcattc ctttcaaaag tatcaccccc
     2161 aaaaacgaca ctctgttaat tacatttgac tccagcgacc agagcgccgc cgcaaaagcg
     2221 gtcctcgaca gaacattgcc ccatggctac atcattgcgc agcaggacaa taacagtcag
     2281 gctatgcagt ggctgacccg gttacgggat aactctcatc gcttcggata acttcctgtt
     2341 atccgaaaca tatcactcac tttggtgatt tcaccgtaac tgtctatgat taatgaggcg
     2401 gtggttaaca ctaccgcttc gttgtctgga caatcgttcc tttgtaacag gtccgaatca
     2461 caatggaagg ttcaagaatg aaataccgca tcgctttagc tgtttctctc tttgctctta
     2521 gtgccggtag ttatgccact accctgtgtc aggaaaagga gcaaaatatc cttaaggaga
     2581 tcagctatgc cgaaaaacac caaaaccaga atcgtattga cggtctgaat aaagccctga
     2641 gtgaagtccg ggccaactgt tcagatagcc agctgcgtgc cgatcatcac aagaaaatcg
     2701 caaagcagaa agatgaggtg gcggaacgcc agcaagattt agccgaggcg aagcaaaaag
     2761 gcgatgccga taagattgcc aaacgcgaac ggaaactggc agaagcgcag gaagagctga
     2821 aaaagctgga agcgcgcgac tactaactca caatagtcac tacttactca cctggagaaa
     2881 actatgtcga aagaacacac tacggaacat ctgcgtgctg agttgaaatc cctttccgat
     2941 acgctggaag aggtgcttag ctcatctggc gagaagtcga aagaagagtt gagtaagatt
     3001 cgtagcaaag cggagcaggc actgaaacag agccgttatc gcctgggtga aaccggtgat
     3061 gccatcgcca aacaaacccg tgtcgcggcg gcgcgtgctg atgagtatgt gcgtgaaaat
     3121 ccgtggaccg gcgtgggaat tggcgctgca atcggtgtag tgctcggcgt tctgctgtcg
     3181 cgtcgttaat tatggcggac actcatcacg cgcaagggcc cggtaaaagc gttctgggca
     3241 tcgggcagcg aattgtttcc atcatggttg aaatggtgga aacacgtctg cggctggcgg
     3301 tggtggagct ggaagaggaa aaagctaatc tctttcaact tttactgatg ctgggcctga
     3361 cgatgctttt cgctgcattt ggtcttatga gcctgatggt gctaattatt tgggcggttg
     3421 atccgcaata tcgcctgaat gcgatgattg ccaccaccgt ggtgttgctg ctactggcac
     3481 tgattggcgg tatctggacg ttacgtaaat cgcgtaaatc caccttgctg cgccatacac
     3541 gccatgagct ggcaaacgat cggcaactgc tcgaggagga gtcccgtgag cagtaaagtc
     3601 gaacgtgaac gacgtaaggc gcaactgctt agccagatcc agcaacaacg gctggatctt
     3661 tccgccagtc gtcgtgaatg gctggaggca acaggcgctt acgatcgtcg ctggaatatg
     3721 ctgctaagtc tgcgctcctg ggcgctggtt ggcagtagcg tgatggcgat ctggacgatt
     3781 cgccatccta atatgctggt ccgctgggcc agacgcggtt ttggcgtatg gagcgcctgg
     3841 cgtctggtta aaacgaccct caagcagcaa cagcttcgcg gttaacttcc ctctggccgg
     3901 agccattccg gccctatccc tcaaattttt tgaagatttt tgacagtttt ccttgctaac
     3961 aatcatcatt caccacgttt atgattctct ccatcgacag caacgacgct aataccgcgc
     4021 ccttgcacaa aaaaacaatc agcagcctga gtggcgcagt ggagagtatg atgaaaaaat
     4081 tagaagatgt tggtgtactg gtagcgcgca ttttaatgcc gattctgttt attaccgcag
     4141 gctggggaaa aatcaccgct tactctggta cccaacaata tatggaagca atgggtgtcc
     4201 cgggttttat gctgccgctg gtgattctgc tcgagttcgg tggtggtctg gcaatcctgt
     4261 tcggtttcct gactcgcacc acagccctgt ttactgcggg ctttacgctg ctgacggcat
     4321 ttttatttca cagcaagttt gctgaaggcg tcaactcgct gatgttcatg aaaaacctga
     4381 caatttctgg cggattcctg ctgctggcaa ttaccggtcc gggcgcgtat agcatcgacc
     4441 gcctgctgaa taaaaagtgg taagcacgcg ctatacttaa cgataaaaaa cgaggaggaa
     4501 gctcctcgtt tttgctattg gaggagagaa aaatggggca actgattgac ggcgtctggc
     4561 atgacacctg gccccactgg cacaggcggt tttatcgcag agaaagatcg ttatcatctc
     4621 tatgtttcac tcgcctgccc gtgggcgcac cgcacgctga tcatgcgcaa actcaaagga
     4681 ctggaaccgt ttatttccgt ttccgtagtg aacccgctga tgctggaaaa cggctggacc
     4741 tttgatgaca gttttccggg agcaaccgga gatacgctct atcaacatga atttctgtat
     4801 cagctttatc tccacgccga tccacactac agcggacgag ttactgttcc cgtgctgtgg
     4861 gacaaaaaga accacaccat tgtcagcaac gaatcagcag aaatcatccg catgttcaat
     4921 accgcgtttg atgcgctggg cgcaaaagcg ggtgattact acccaccagc cctgcaaacg
     4981 aaaattgacg aacttaacgg ctggatttat gacaccgtta acaacggcgt gtataaagct
     5041 ggttttgcca ccagccagca agcctacgac gaggcggtgg cgaaagtgtt tgaatcgctg
     5101 gcgcgactgg agcagatttt aggccagcat cgttacctga ccggcaacca gctaaccgaa
     5161 gccgatattc gcctgtggac tacgctggtg cgttttgatc cagtgtatgt gacccacttc
     5221 aagtgtgata agcaccacat cagcgattac ctgaatctgt atggcttcct gcgcgatatc
     5281 taccagatgc cggggatcgc cgaaactgtc aatttcgatc acatccgtaa tcattacttc
     5341 cgcagccata agaccatcaa ccctacgggg attatttcaa ttggtccgtg gcaggatctc
     5401 gatgaaccgc atggacgaga tgttcgcttc ggttaaaaaa aggcacctta cgaggtgcct
     5461 ctgataatta gctaagtgca tgttgttcat gtcggatgcg gcgtgaacgc cttatcaaac
     5521 ctacaaaacg tttatattca atatgttaaa atgaccgcgt aggccagata agcgtagcgc
     5581 atctggcgat tttgcgcttg tcatcagtct tagcgcacct tcacggcact ttattaattc
     5641 cttgaacgaa tatttactgc catttcattc acgtctattc ttaatttgct gctttaaaaa
     5701 caagtgattg agcaaaattg aggcaaaaat ggactggtat ctgaaagtac taaaaaatta
     5761 tgtcggtttc cggggtcgtg cgcggcgcaa agagtactgg atgtttattc tggtcaacat
     5821 catctttacg ttcgtgctgg ggctgctgga taaaatgtta ggctggcaac gcgccggtgg
     5881 cgaaggcatc ctgacgacaa tctacggtat tctggtgttt ttaccgtggt gggcggttca
     5941 gttccgccgc ctgcacgaca ccgaccgctc ggcgtggtgg gcactgttgt tcttaatccc
     6001 gtttatcggc tggcttatta tcatcgtctt taactgccag gcgggtacgc caggcgaaaa
     6061 ccgctttgga ccagatccga aactggaacc ttaaaacaaa tctgctcgcc ggatgtctga
     6121 ctcatccggc ttttatcatc atgtt
//
LOCUS       DAZPEB010000206         6051 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZPEB010000206 DAZPEB010000000
VERSION     DAZPEB010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6051
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.211"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(95..2197)
                     /gene="pqqU"
                     /locus_tag="ACHURJ_003639"
     CDS             complement(95..2197)
                     /gene="pqqU"
                     /locus_tag="ACHURJ_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFR1282424.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            2439..3500
                     /locus_tag="ACHURJ_003640"
     CDS             2439..3500
                     /locus_tag="ACHURJ_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFR1282425.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(3613..5112)
                     /gene="ansP"
                     /locus_tag="ACHURJ_003641"
     CDS             complement(3613..5112)
                     /gene="ansP"
                     /locus_tag="ACHURJ_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFR1282426.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            5379..5996
                     /locus_tag="ACHURJ_003642"
     CDS             5379..5996
                     /locus_tag="ACHURJ_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFR1282427.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNATNISAIADAVCQLPKLQEVLKRNEII"
BASE COUNT         1611 a         1537 c         1443 g         1460 t
ORIGIN      
        1 gtcttgctga aattatgcaa caaaatattg ctattttata ccagcaatac aatcgttaaa
       61 gtaaatgccc ggcaaatacc gggcatcaag gtaattactc aaatctccac gcaatattca
      121 tgccgacacc ataatttcgt ccgggcgaag gttcgtaata tctcccgttt gactcattga
      181 caatgacaga accaacgtat tctttatcga ataaattatc gacacgacca aataaatcca
      241 cagttaaatt gtggtaattg tatttatacc cggtgaataa gccgacgaga gtataagacg
      301 gcgcttttgc cgtattttca tcatctgcca taatatcgcc catataacgc gcttccgtgc
      361 ctgcatacca accatcttcc ggtacataac ctatcgacgc aaagcccata ttacgggcga
      421 tccctggcat ccgattaccg ttacaatcct gttcattgca aacattgctg cgataggtcg
      481 catcaagcca ggtccaggac gcgtttacgc gaaaatctcc tgcgaaacgt tgatcccatg
      541 ccagttcagc gccttgacga cgggtctttc cggcattttt gtaagtcgta cgcccaccgc
      601 tactgctatc gacaacaatt tcatcatcag tgtcggtctg aaacaatgcg agactaagca
      661 gcccatcacc aatacgcgtt ttactgccga tctcaattgt atcgttggtg gatggtttta
      721 aaccaaagtt cataccgctt tgcccatcag cacgataaga cagctcatta atcgtcggcg
      781 tttcaaaacc tcgcccggct gccagataga tattccaggc atcggtcatt gcatatttta
      841 acgaaccggc aggtagccat ttatgataac tggcatcacc gctgtcatcg ccgttacccg
      901 gagtaacgta atggtcgttg gaatcaaacc acacggagct gtagcgcacg ccagcatcca
      961 gcgacagttt ttcgctcagc tgccactgcg tctgtaaata gggatcgatg ttccacatca
     1021 gattgcgttc gtcgcgacgc aactcacctt tttgcccgta ctccggcatg ccgctattca
     1081 ggcggaagtt attgtagccc ttgcggtttt cactcatgtt ttcgtagttc aggccggtag
     1141 tgaacgtgac cggaacgccc agttcaccac ggtgtgtcca gcggctgtct atcccctggt
     1201 aatggcgttg cagggtaatc acgccgcccg catgtgacgg gttaagttgt ggtgccatgg
     1261 gtattgactg gtactgggtc gtttctcgct ctccggcata catcatcaca ctcatatcat
     1321 cttgcgcgct caggctacgc tcatagcgca acccagcctg agtttgcttg atggtttttc
     1381 gcgtgtcgta ctgttctgca cgaggcgctt gttgtggatt agccttccat tctgctttgg
     1441 ttagcccacc tgggtcatct gctttgatat ccacactatt gaaaatcaga cttaatttgc
     1501 tggcttcatc aatgcgtacg cccagtttgg cattggctaa atttttctgt gcgccactat
     1561 ggtcacgata gccgtgggtc gtaaaacgcg tggttgagac ggtgtaatcg acatcgccag
     1621 gctgtgtgcc gtctcccgtt gcgcccgttg ctttcagccc atagcgccag ctgccaaaac
     1681 tgccgtagta actactggct tcaatggttg gtggctgttg tccggtctgg gtggtgacat
     1741 tcattacccc accagacgcg ttgccataca gggcagagaa ggggccacgc agcacttcca
     1801 cattttgcac actgcttaaa tcgatgttgg atgtttgccc ttgcccgtcg ggcatggtgg
     1861 cgggaatacc gtccacatac aggcgaatac cgcgaatacc gtaagtggag cgggagccaa
     1921 atccgcgaat cgacagctgt aaatcttgcg catagttctg ccggttttgt acctgcaaac
     1981 caggcacgcc ggtcagtgat tcggacaagt taatgcgcgg tgttgccagg cgcatctcct
     2041 cgccatccac cacgcttact gctgctgggg tatccagttc tgaaaccacc tgcggtgcgg
     2101 cactgacaat catagtctgt tcatcagcgg caaaaacaac gggggaaagg acaagcagtg
     2161 cgggcaaaac ggtctgtcgg acggaaaaaa tcttcatgaa aaaagccagg ttaagaatgg
     2221 gaaaacgctg tcatggtaat aaaattgtaa acttatgcaa aacgaaacgg cacgatacgt
     2281 taattaattg agaaaattgt agtcgtaacg gcaagaaatg ctccacattt gagaaaataa
     2341 tgattaccat tcccatttat aacaagagcg tgacgatgat tacgcttagc gaagcattgt
     2401 gaagcagcaa aaatattggt tcatcaaagg gagtcgtcat gcatttacgt catctgtttt
     2461 catcgcgcct gcgtggttca ttactgttag gttcattgct tgttgcttca tcattcagta
     2521 cgcaggccgc agaagaaatg ctgcgtaaag cggtaggtaa aggtgcctac gaaatggctt
     2581 atagccagca agaaaacgcg ctgtggctcg ccacttcgca aagccgcaaa ctggataaag
     2641 gtggcgtggt ttatcgtctt gatccggtca ctctggaagt gacgcaggcg atccataacg
     2701 atctcaagcc gtttggtgcc accatcaata acacgactca gacgttgtgg tttggtaaca
     2761 ccgtaaacag cgcggtcacg gcgatagatg ccaaaacggg cgaagtgaaa ggccgtctgg
     2821 tgctggatga tcgtaagcgc acggaagagg tgcgcccgct gcaaccgcgt gagctggtag
     2881 ctgatgatgc cacgaacacc gtttacatca gtggtattgg taaagagagc gtgatttggg
     2941 tcgttgatgg cgagaatatc aaactgaaaa ccgccatcca gaacaccggt aaaatgagta
     3001 ccggtctggc gcttgatagc aaaggcaaac gtctttacac cactaacgct gacggcgaat
     3061 tgattaccat cgacaccgcc gacaataaaa tcctcagccg taaaaagctg ctggatgacg
     3121 gcaaagagca cttctttatc aacatcagcc ttgataccgc caggcagcgt gcatttatca
     3181 ccgattctaa agcggcagaa gtgttagtgg tcgatacccg taatggcaat attctggcga
     3241 aggttgcggc accggaatca ctggctgtgc tgtttaaccc cgcgcgtaat gaagcctacg
     3301 taacgcatcg tcaggcaggt aaagtcagtg tgattgacgc gaaaagctat aaagtggtga
     3361 aaacgttcga tacgccgact catccaaaca gcctggcgct gtctgccgat ggcaaaacgc
     3421 tgtatgtcag tgtgaaacaa aaatccacta aacagcagga agctacccag ccagacgatg
     3481 tgattcgtat tgcgctgtaa tagatatggc gagggtgaaa acggtgagac ttgcctgcct
     3541 tttatcacgg cgtatccggc actttcgtca gtaatctgaa tttgcccggc acgtagccgg
     3601 gcaattttgc aattaactcg ccgtttcagg cttaaacaca atttcctgtt tttcttcatc
     3661 ttcctcgacg actggcgcag tgctgtgaat ttcagcaacg cgtttgcgca caccaaacca
     3721 gcctataacc agcagaatac cgataatcgg cagcgccgcg atagtataag tcccgttcgg
     3781 gtaatcgaac gccatcagca caaggacact cagtaaaaac agtaatgtca gccaggaagt
     3841 gaagggcgcg ccaggcagtt taaaactgac atccgctgct ttgccttctt taatcgcttt
     3901 acgcaggcgc atctggcaca cgatgataaa cgcccatgaa gcgatgattc ccagcgacgc
     3961 gaagttcaac acaatctcaa atacgcgcga cggcaccaga tagttgagga atacgccgac
     4021 gacatacaca accagtgtcg ctagaatccc ggcatacggc acatgctgac gactcatttt
     4081 cgccataaaa ctcggtgcgg aaccgcccat cgccattgag cgcagaatac gtccggtgca
     4141 gtacagacct gagttcaggc tggagagggc agcggtcagc accacaatgt tcataatgct
     4201 gccgatatat ggcacaccca gtttagagaa aaacgtcacg aacggacttt gccccgcctg
     4261 atacgcgctc cacggcaata acataaccag caacaccacg gagccgacgt aaaacaggcc
     4321 aatacgccaa atcacactgt taatggcttt aggcaccatg gtctgcggat ctttacattc
     4381 tccggcagct gtacccacca tttcaatgga ggcaaaagca aacactacgc cctgaatcaa
     4441 caccagcgca ggcagcaaac cgtgggggaa gaagccgcca ttatcggtga ttaaatgaaa
     4501 gccagtggtg ttgccatcca gcggctgacc actaccgagg aacactgtac ccacgaccaa
     4561 aaaggtcaca atggcgagca ctttaataag cgcaaaccag aactccatct ccgcaaacca
     4621 tttcacaccg atcatattca tggtgccaac gatggtaagt gcagcgagcg caaagaccca
     4681 ctgcggcacg ccgccaaacg caccccagta atgcatatac agagcgacgg cggtaatatc
     4741 aacaatcccc gtcatcgccc agttgatgaa gtacatccag ccagcaacat aagcggcttt
     4801 ctcacccaaa aactcacggg cataagagac aaaactgcca ctggaagggc ggtgtagcac
     4861 cagctcaccc aatgcacgca gaataaaaaa cgaaaacaag ccacaaatta aataaaccag
     4921 tgccagtgct ggacccgcca tttgcagtcg ggctcctgca cctaaaaaca agccggtgcc
     4981 aatcgcgccg ccaatggcga tcatctgtac ctggcgattg cccatcgctt tgtgataccc
     5041 ctcttcgtgg gcattaagcc agcggcgttt cgcggcgtgt tgatctgaag tgtcggtgtc
     5101 gtgtttactc attgctctcc ctgattgctt taatgaaaaa gtcatataag ttgccatgaa
     5161 caatgtttat tcatcctggc gatgatagtt atcaccatta ttctttatgg gtataaaact
     5221 atcggcgcag catcctaccc gttatcgata tacgatgcaa aacatcccct tacaatcctg
     5281 aaggggattc atacaactga cgaaaaaatg acaaatactt ttgctggtta acctgtgtac
     5341 tgtcctacac ttaatcttta aaagattgtg aggggcatat gattaaggtg tatggcgtac
     5401 ccggatgggg ctctacaatc agtgagctga tgctaacact ggctgatatt ccttatcagt
     5461 tcgttgatgt cagtggtttt gaccacgagg gaacttcacg cgagttattg aaaaccctga
     5521 acccgttgtg tcagattccg acactggcgc tggaaaatga cgagattatg acggagacag
     5581 cggcgattgc attgatggtt ctcgatcgtc gcccggacct tgcgccaccg gttgggcgcg
     5641 ccgaacgtca gcagtttcaa cggctattgg tctggctggt tgctaatgtc tatccaacat
     5701 tcactttcgc cgattaccct gagcgttggg ctcctgacgc acctgaacag ttaaagaaga
     5761 atgttattga atatcgtaaa tcgctttata tctggctgaa ttcacaattg accgctgaac
     5821 cttatgcgtt tggtgagcaa ctaacgctgg tggactgtta tctttgcact atgcgcacat
     5881 gggggcctgg gcatgaatgg tttcaggata atgccacgaa tatcagtgcg attgctgatg
     5941 ctgtatgtca gttaccgaaa ttacaagaag tattaaaaag gaatgaaatt atttgagtgg
     6001 gataccagag gattatgtta tttattattc agtaacttta tccacaatga t
//
LOCUS       DAZPEB010000207         5885 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZPEB010000207 DAZPEB010000000
VERSION     DAZPEB010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5885
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.212"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            160..723
                     /locus_tag="ACHURJ_003643"
                     /pseudo
     CDS             160..723
                     /locus_tag="ACHURJ_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            805..3447
                     /gene="yfcU"
                     /locus_tag="ACHURJ_003644"
     CDS             805..3447
                     /gene="yfcU"
                     /locus_tag="ACHURJ_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HFR1282428.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVGG
                     SVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQIRYKIMMGRPQEWGHHVEGEFFSGA
                     EASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYGK
                     GSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKEM
                     CTATYNQNFRDAGVSVYLNYTRHTYWDREEQINYNIMLSHYFNMGSIRNVSISMTGYR
                     YEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVGT
                     SDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMG
                     GTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNRLPENAEAT
                     QSVVQATLTEGAIGYRKFAVISGQKAMAVLRLSDGSHPPFGAEVKNDNEQTVGLVDDD
                     GNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPITSPAV
                     KPAIQEQTQRVTPTEPPTSISVNQ"
     gene            3467..4219
                     /locus_tag="ACHURJ_003645"
     CDS             3467..4219
                     /locus_tag="ACHURJ_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFR1282429.1"
                     /translation="MFNLTNTAKIVVPALALLATAVSFSSHASVTPDRTRLVFNESDK
                     SISVTLRNNDPTLPYLAQSWIEDAKGNKISSPLAVLPPVQRIDAMMNGQVKVQGLPDI
                     NKLPADRESVFYFNVREISPKSNKPNTLQIALQTRIKLFWRPKALENTSMKSPWQHKV
                     TLIRNGQDFTVNNPTPYYVIISNASAKKGGNPPAGFTPLMMEPKTSAPLNAKMDSVPV
                     LTYINDYGARMPLYFKCTGNSCQVDEEQSRKG"
     gene            4235..4744
                     /locus_tag="ACHURJ_003646"
     CDS             4235..4744
                     /locus_tag="ACHURJ_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1282430.1"
                     /translation="MSHKFVAGAGLLVLAGWGISGLAVAGSDIKQVDLTLRILVDGPP
                     PCSVKGSAVKFGNVIINRIDGTNYQQDAKYTLNCGNKVSDDLRMQLKGNTAVINGETV
                     LSTDIAGLGVRIENAADNSLFAVGENNWTPFNVNTQPQLKAVPVKQSGVQLTPSEFNA
                     TMTMVVDYQ"
     gene            4741..5229
                     /locus_tag="ACHURJ_003647"
     CDS             4741..5229
                     /locus_tag="ACHURJ_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1282431.1"
                     /translation="MKKFLLLLLCAGCGAVLAAEDDITFHGTLMSPPACSISGGQTIE
                     VQFRDLIIDSIDGNYGRQKVDYELSCDSDVRDPEWNMTLSWTGTATSYNDSAIETDVP
                     GFGIELQHDGQRFKLNEPLSINATDFSQKPKLEAVPVKAADAVLTDTTFSAYATLRVD
                     YQ"
     gene            5226..5765
                     /locus_tag="ACHURJ_003648"
                     /pseudo
     CDS             5226..5765
                     /locus_tag="ACHURJ_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416836.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
BASE COUNT         1513 a         1446 c         1580 g         1346 t
ORIGIN      
        1 atcactatgc ggccccacac caacgaatac aattttctac gacctgttct ttttggatcg
       61 catgccgtat tcaatggctg ggtataaaca ttggctattg actgaatcaa tgtattgcaa
      121 tgtttatatt tttaattatt tatagtttaa ggacaaataa tgagtaagtt tgttaaaaca
      181 gctattgctg cggcaatggt gatgggcgtg ttcacctcta cggcaacaat cgctgcgggt
      241 aacaacggta ctgcacgttt ttacggcacc attgaagact cagtgtgttc tatcgttccg
      301 gacgatcaca aactggaagt tgatatgggt gatatcggtg ccgaaaaact gaaaaataac
      361 ggcaccacca cgccgaaaag tttccagatt cgtctgcaag attgcgtatt tgatacttag
      421 gaaacaatga ccactacctt tactggtacc gtttcttctg caaatagcgg caattattac
      481 accattttca ataccgatac cggtgcggca tttaacaatg ttagcctggc gatcggtgac
      541 tctctgggta cctcttacaa aagcggcatg ggtattgacc agaaaatagt gaaagacact
      601 tctactaata aaggcaaagc gaagcagaca ctgaacttta aagcctggct ggtcggcgca
      661 gctgatgcgc cagatctggg taatttcgaa gcaaatacca ccttccagat tacttacctg
      721 taaggcatac ccccatagcg acactgctat gggggattta aatggatttt gaccgaacaa
      781 gaataaaaac ggatagaaac gtgtatgcct gaccattctc tttttcgatt acggattctt
      841 ccgtggtgca ttgcgctggc aatgtcaggg agttatagca gtgtctgggc tgaagacgac
      901 attcagtttg attcccgttt tctggaatta aaaggcgaca caaaaattga tctgaagcgt
      961 ttttccagcc agggatatgt tgagcccgga aaatacaatt tacaggttca actaaataaa
     1021 cagccattgg cggaagagta cgatatttac tggtatgctg gtgaagatga cgcgagcaaa
     1081 agctatgctt gtctgacacc ggaactggta gcgcagtttg gtttaaaaga agacgtggcg
     1141 aaaaatctgc aatggagcca cgatgctaaa tgcctgaaat ccggtcaact ggaaggcatg
     1201 gaaattaagg ctgatttaag ccagtccgca ttagtcattt cactgccaca ggcttacctc
     1261 gaatatactt atcccgactg ggatccgcct tcacgttggg atgacggcat ctccgggatc
     1321 gtcgcggact acagcatcaa cgcacaaacc cggcacgaag aaaatggcgg tgatgatagt
     1381 aacgagatca gcggcaacgg gacggtcggg gttaacctgg ggccgtggcg tatgcgtgct
     1441 gactggcaga ctaactatca acatactcgc agtaatgatg acgatgaatt cagcggcgat
     1501 gaaactcaaa aaaaatggga gtggagtcgc tactatgcct ggcgggcgtt accatcatta
     1561 aaagccaaac tggcgctggg cgaggattac ctcagatccg atatttttga tggttttaac
     1621 tatgttggtg gtagtgtcag tactgacgat caaatgttgc ctcccaatct gcgcggctac
     1681 gcgccagaca tttccggcgt ggcacacacc acagcaaaag tgaccgtcag ccagatgggg
     1741 cgtgtgattt acgaaacgca ggttccggct ggaccgtttc gtattcagga tcttggtgat
     1801 tctgtctccg gtacgttgca tattcgcatt gaagaacaga acggccaggt gcaggaatat
     1861 gacatcagca ccgcctcgat gccatacctc actcgtccag gtcagattcg ctataagatc
     1921 atgatgggcc gtccgcaaga gtggggacac catgtcgagg gtgaattttt ttctggtgct
     1981 gaagcttcct gggggatcgc taacggctgg tcgttatatg gcggcgcact gggagatgaa
     2041 aactatcagt ctgcggcgct tggcgtcggt cgcgatttgt ctacattcgg cgcggtcgcg
     2101 tttgatgtta ctcactcgca caccaaactg gataaagaca ccgcttatgg caaaggttcg
     2161 ctggacggta actccttccg tgtgagttat tccaaagact ttgaccagct caacagccgc
     2221 gttacttttg ctggatatcg cttctcggaa gagaacttta tgaccatgag cgagtatctg
     2281 gatgccagtg acagcggaat ggtacgcacg ggcaacgaca aagagatgtg caccgccact
     2341 tataaccaga acttccgcga tgcgggtgtt tcggtttatc tcaactatac ccgccatacc
     2401 tactgggatc gcgaggagca gataaactac aacatcatgc tctcgcacta tttcaatatg
     2461 ggcagtattc gtaatgtcag catctcgatg actggctacc gttacgagta tgacaaccag
     2521 gccgacaaag gcatgtacat ttcgctcagt atgccgtggg gcgacaacag taccgttagc
     2581 tataacggta actatggcag tgggacggac agcagtcagg tcggttattt cagccgtgtc
     2641 gatgacgcga ctcactatca gttgaacgtc ggcaccagtg acaaacacac cagcgttgac
     2701 ggctattaca gccatgatgg ttcgctggcg caggttgacc tcagtgcgaa ctaccatgaa
     2761 gggcaataca cctctgcggg cttgtcgtta cagggcggcg caacgcttac tgcccacggt
     2821 ggcgcacttc accgtaccca gaatatgggc gggacacgct tgttgattga tgccgatggc
     2881 gttgccgatg ttccggtgga aggtaacggg gctgctgttt ataccaatat gtttggtaaa
     2941 gccgtcgttt ctgacgtcaa taactattac cgcaatcagg cgtatatcga cctcaacaga
     3001 ttgcctgaaa acgctgaagc aacccagtcg gtggtgcaag ccacgctaac tgaaggtgcc
     3061 attggctacc gtaaatttgc tgtcattagc ggacaaaaag cgatggcggt gctgcgcctg
     3121 agcgacggca gccatcctcc gtttggcgca gaagtaaaaa atgataacga gcagacagtg
     3181 ggccttgtcg atgatgacgg caatgtttat ctggctgggg tgaaacctgg cgaacacatg
     3241 agtgtgttct ggagtggtgt tgcgcattgc gatatcaacc tgccggaccc gctgcctgcc
     3301 gatctgttta acggcttgtt actaccatgc cagcataaag gcaatgtagc acctatcact
     3361 tcgccggcgg tcaaaccggc gattcaggaa cagacacagc gggtgacgcc aacggaacct
     3421 ccgacttcaa tttcagtaaa ccagtaacgt gattaaggaa tgatccatgt ttaatctgac
     3481 taacactgca aaaatcgttg tcccggcact ggcgctgctg gcgacagcgg tcagtttctc
     3541 cagccacgct tcggtgacgc ccgacaggac acggctggtg tttaacgaaa gcgacaagtc
     3601 catcagcgtc acgctgcgca acaacgatcc cacactgcct tatctggcgc agagctggat
     3661 agaggatgca aagggcaaca aaatcagctc tccgcttgcg gtgcttccgc cagtgcagcg
     3721 catcgatgcg atgatgaacg gccaggtgaa ggtgcagggg ctgccggata tcaacaaact
     3781 gccggccgac cgcgagagcg tgttctattt caacgtgcgc gaaatttcgc cgaaatcaaa
     3841 taaaccgaac acactgcaga ttgcgctgca gacgcgtatc aaactgttct ggcgaccgaa
     3901 ggcgctggag aacaccagca tgaaaagtcc gtggcagcat aaggtgacgc tcatccgtaa
     3961 cggccaggac ttcaccgtca ataacccgac gccgtactac gtgatcatca gcaacgccag
     4021 cgctaaaaaa ggcggcaacc cgccggcggg attcacaccg ctgatgatgg agccgaaaac
     4081 ctccgcaccg ctgaatgcga agatggacag cgtgccggtg ctgacttaca tcaacgacta
     4141 cggcgcgcgg atgccgctgt acttcaaatg caccggcaac agctgccagg tggatgaaga
     4201 gcagagccgc aaaggctgat gccgggaggg acttatgagt cacaagtttg tagcgggcgc
     4261 agggttgctg gtgctggccg gatggggcat cagcggtttg gctgttgctg gcagtgatat
     4321 caaacaggtg gatctgacgc tgcgaatcct agtcgatgga ccgccgcctt gctccgtgaa
     4381 gggaagcgct gtgaaatttg gcaatgtgat aatcaacagg atcgatggta ccaattacca
     4441 acaggatgcg aaatacaccc tcaactgcgg taataaagtc agcgatgact tacggatgca
     4501 gctcaaaggc aataccgccg tgatcaacgg agaaacggtt ctcagcacgg atatcgccgg
     4561 cctgggggtg cggatagaaa acgccgccga caactccctg tttgcagttg gtgaaaataa
     4621 ctggacgcct tttaatgtca acactcagcc tcagctgaaa gcggtgccgg taaaacagag
     4681 cggggtgcaa ctgactccgt cggagtttaa cgccacgatg accatggtgg tggattacca
     4741 atgaaaaaat tcctgctcct tttactgtgc gctggttgcg gggctgtgct ggcggcggaa
     4801 gatgacatca cattccacgg tacgctgatg tcgccgcccg cctgtagcat cagcggcggg
     4861 caaaccatcg aagtgcaatt tcgcgatctc attatcgaca gcattgatgg caattatggt
     4921 cgtcagaaag tggactacga actgagctgc gactcggatg tccgcgatcc cgagtggaat
     4981 atgacactct cctggacggg gactgccacc tcctacaacg actctgctat tgagacggat
     5041 gtaccgggct tcggaattga gcttcagcat gacggtcagc gttttaagct caacgagccg
     5101 ctttccatta acgccaccga ttttagccag aagccaaagc tggaggcggt accggtcaaa
     5161 gcggctgatg cggtgctgac ggacacgacc ttttctgctt acgcaacctt acgggtggat
     5221 taccaatgaa aacagcacac atttattcac ccggttatgc gctgttagca ggcctgctgc
     5281 tgaccgccag cacaggagca caggcagtcg acaacaacct gcatttttac ggcaacctgc
     5341 tgagtaaatc ctgcacgctg gtggtggatg gtgccaacct ggcagaaatc cattttccca
     5401 ccgtcagcaa ccgggatttg atggtatctg ggcagtccgc gcatctgccg gttgtgttta
     5461 agctgaaaga ctgcaaaggg ccggcgggat acaacgtcaa agtcacgctg agcggcaccg
     5521 aagacagcgg tcagccgggt tttctggcag tcgatgacac ctccaccgcg cagggcgtgg
     5581 ggatagggat ggaaacgctc gacggcaagc gggtggatat taacaacacc accggggcga
     5641 cctttgcgct taccaacggc aacaatgata ttaatttccg ggcctggatc taggcgaaaa
     5701 gcggtcgtga tgtaaccatc ggtgatttca ccgccagcct gacagcaact tttgagtata
     5761 tttaaaatga aaatccttcg tcatgtttta tgtcttttat tgctgttaat gttcaataca
     5821 tgcggccagg cggcgacgaa agttggtgag ttatatgtgc cagctggtgc cagttcacct
     5881 gcgac
//
LOCUS       DAZPEB010000208         5816 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZPEB010000208 DAZPEB010000000
VERSION     DAZPEB010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5816
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.213"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..857
                     /gene="pdeC"
                     /locus_tag="ACHURJ_003649"
     CDS             <1..857
                     /gene="pdeC"
                     /locus_tag="ACHURJ_003649"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFR1282432.1"
                     /translation="FALPAGILGSLVLLLLWLRIRQNYLSPKRKLQRALEKHQLCLYY
                     QPIIDIKTEKCIGAEALLRWPSEQEQVMNPAEFIPLAEKEGMIEQVTDYVIDNVFRDL
                     GDYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKFEVTEHAFLDVEK
                     MTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVETLTTHKTSHLIA
                     EHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMPPQVFMQWMEQLP
                     ARELTRGQ"
     gene            complement(860..1183)
                     /gene="soxS"
                     /locus_tag="ACHURJ_003650"
     CDS             complement(860..1183)
                     /gene="soxS"
                     /locus_tag="ACHURJ_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFR1282433.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            1269..1733
                     /gene="soxR"
                     /locus_tag="ACHURJ_003651"
     CDS             1269..1733
                     /gene="soxR"
                     /locus_tag="ACHURJ_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFR1282434.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYRRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            2279..3628
                     /gene="ghxP"
                     /locus_tag="ACHURJ_003652"
                     /gene_synonym="yjcD"
     CDS             2279..3628
                     /gene="ghxP"
                     /locus_tag="ACHURJ_003652"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFR1282435.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFGMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            3780..5429
                     /locus_tag="ACHURJ_003653"
     CDS             3780..5429
                     /locus_tag="ACHURJ_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFR1282436.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(5583..>5816)
                     /locus_tag="ACHURJ_003654"
     CDS             complement(5583..>5816)
                     /locus_tag="ACHURJ_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR1282437.1"
                     /translation="MFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVTLQKFF
                     EEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
BASE COUNT         1257 a         1376 c         1603 g         1580 t
ORIGIN      
        1 tttttgcgtt gcccgccggt attttgggga gtcttgttct gctattactc tggctacgta
       61 ttcgacaaaa ctatttgtct cccaaacgca aattgcaacg cgctctagaa aaacatcaac
      121 tttgtcttta ttaccagcca ataatcgata tcaaaacaga aaaatgtatc ggcgctgaag
      181 cgttgttacg ttggcctagt gagcaggagc aagtaatgaa tccggcagag tttattccgc
      241 tggcagaaaa ggaggggatg atagaacagg taactgatta tgttattgat aatgtcttcc
      301 gcgatctggg cgattacctg gcaacacatg cagatcgcta tgtttctatt aacctgtcgg
      361 cctccgattt tcatacgtca cggttgatag cgcgaatcaa tcagaaaaca gagcaatacg
      421 cggtgcgtcc gcagcaaatt aaatttgaag tgactgagca tgcatttctt gatgtcgaaa
      481 aaatgacgcc gattattctg gctttccgcc aggctggtta cgaagtggca attgatgatt
      541 ttggtattgg ctactctaac ttgcataacc ttaaatcatt gaatgtcgat attttgaaaa
      601 tcgataaatc gtttgttgaa acgctgacca cccacaaaac cagtcatttg attgcggaac
      661 acatcatcga gctggcgcac agcctggggt taaaaacgat cgctgaaggc gtcgaaactg
      721 aggagcaggt taactggctg cgcaaacgcg gcgtgcgcta ttgccaggga tggttttttg
      781 cgaaggcgat gccgccgcag gtgtttatgc aatggatgga gcaattaccc gcgcgggagt
      841 taacgcgcgg gcaataaaac tacaggcggt ggcgataatc gctgggagtg cgatcaaact
      901 gccgacggaa aacgcgggag aaggtctgct gcgagacata acccaggtcc attgcgatat
      961 caaaaatcgg acgctcggtg gtgcgcaact caacggcggc cagtaacagg cgacgttggc
     1021 gaatgtaatc gccaagcgtc tgatgcgtca ccgtgcggaa cattcgttgc aagtaccact
     1081 ttgaatagcc tgattttttt gcgactacat caatgttaag cggctggtca atatgctcgt
     1141 caatccatgc gataagatcc tgaataattt tctgatggga cataaatctg cctcttttca
     1201 gtgttcagtt cgttaattca tctgttgggg agtataattc ctcaagttaa cttgaggtaa
     1261 agcgatttat ggaaaagaaa ttaccccgca ttaaagcgct gctaaccccc ggcgaagtgg
     1321 cgaaacgcag cggtgtggcg gtatcggcgc tgcatttcta tgaaagtaaa gggttgatta
     1381 ccagtatccg taacagcggc aatcagcggc gatatagacg tgatgtgttg cgatatgttg
     1441 caattatcaa aattgctcag cgtattggca ttccgctggc gaccattggt gaagcgtttg
     1501 gcgtgttgcc agaagggcat acgttaagtg cgaaagagtg gaaacagctt tcgtcccaat
     1561 ggcgagaaga gttggatcgg cgcattcata ccttagtggc gctgcgtgac gaactggacg
     1621 gatgtattgg ttgtggctgc ctttcgcgca gtgattgccc gttgcgtaac ccgggcgacc
     1681 gcttaggaga agaaggtacc ggcgcacgct tgctggaaga tgaacaaaac taaagcgcca
     1741 caagggcgct ttagtttgtt ttccggtctt tgtctttctc tctatcccgc tggtacacag
     1801 gagggtttcc cccgacgtca acacacctca ttcgagcacg tggtggaggt tccggttggt
     1861 gttgatgctt taattgtatg tcaccgacgt ttcttcgcca gtgtaaaagt atacttttta
     1921 accgcaatat ttttgtcatc tcagacgatt ttttatcgca atcctgaacg gtatacggct
     1981 cgataacgct gcaatcttgc gcaccgacga taacgtttgc gcatcaattg cctgtttttt
     2041 catcgtcaag acaataaaag agaaaaaagc agcaaacttc ggttgaaaaa gccgctatga
     2101 tcgccggata atcgtttgct ttttttacca cccgttttgt atgcgcggag ctaaacgttt
     2161 gcttttttgc gacgcagtaa attgtcgcaa acctggagca ggaagataac gtttcgctgg
     2221 caggggattg tccgccacgc atcttgacga aaattaaact ctcaggggat gttttcttat
     2281 gtctacgcca tcagcgcgta ccggcggttc actcgacgcc tggtttaaaa tttcacaacg
     2341 tggaagcact gtccgtcagg aagtggttgc cgggttaaca acgtttctgg cgatggtcta
     2401 ctcggtcatc gtcgttccag gtatgttggg taaagcaggc ttcccgcctg cggcagtttt
     2461 cgttgcaacc tgtctggttg ccggactcgg ttctatcgtg atgggtctgt gggctaatct
     2521 gccgttggcg attggttgcg ccatctccct gacggcgttt accgcattca gcctggtgct
     2581 ggggcaacat attagcgtac ctgtcgcgct gggtgccgtg ttcctgatgg gtgtgctgtt
     2641 tacggtaatt tctgccacgg gtatccgtag ctggattttg cgcaacttgc cgcacggtgt
     2701 ggcgcacggc acggggatcg gtatcggtct gttcctgctg ctcattgccg ctaatggtgt
     2761 tggtctggtg attaaaaacc cgcttgatgg tctgcccgtt gcgctgggtg atttcgcgac
     2821 cttcccggtg attatgtcac tggtaggtct ggcggtgatc atcggcctgg aaaaactgaa
     2881 agtccctggt ggcattctgc tgaccattat cggtatctca attgtcggtt tgatcttcga
     2941 tcctaacgtc catttctccg gcgttttcgg catgccttca ttgagcgatg aaaacggcaa
     3001 ttcactgatt ggcagcctgg acattatggg cgcgctgaat cctgtagtcc tgccaagcgt
     3061 tctggcgctg gtgatgacgg cagtatttga tgccaccgga actatccgtg ccgtcgccgg
     3121 ccaggcgaac ctgctggata aagatgggca gatcatcgac ggtgggaaag cactgaccac
     3181 tgactccatg agcagcgttt tctctggcct ggtgggtgct gctccggcag cggtatacat
     3241 cgagtctgcg gcgggtacgg cggcgggcgg taaaaccggt ttgacggcta tcaccgttgg
     3301 cgtgctgttc ctgctgattc tgttcctctc tccgctctct tacctcgttc cggggtatgc
     3361 aacggctccg gcgctgatgt acgttggcct gctgatgctg agcaacgtgg cgaaaatcga
     3421 ctttgctgat tttgttgatg cgatggcggg tctggttacg gcggtattca tcgtgctgac
     3481 ctgtaacatc gtaacaggca tcatgatcgg cttcgcgact ctggtgattg gtcgtctggt
     3541 ttccggcgaa tggcgcaagt tgaacatcgg tacggtcgtt atcgccgtgg cgctggtgac
     3601 cttctatgcg ggtggctggg ctatctaatc tttcatcgga ttctgaaaac gggtggcaat
     3661 ggctgcccgt ttttattttc tccgcacatt gtgtgagctt ttgcgatatt ctgaaaaaat
     3721 gagaattcag gcataacgcc tttctcagat cataaaaaaa catcgcaaac agggaacgca
     3781 tggaaatctt cttcaccata ctgataatga ccctcgtggt ctcgctgtcc ggggtggtca
     3841 ctcgtgtcat gccctttcag atcccgcttc cgcttatgca aatcgccatc ggtgcgctac
     3901 tggcgtggcc gacgtttggt ttgcatgtgg agtttgatcc tgaactcttt ttagtcttgt
     3961 ttatcccgcc gttgctgttc gctgatggct ggaaaacgcc gacccgtgaa tttctcgaac
     4021 atggtcgaga gattttcggc ctcgcgctgg cgctggtggt ggtcaccgtg gtcggcattg
     4081 gcttccttat ttactgggtg gtgccgggca ttccgctgat ccccgccttt gcgctggcgg
     4141 cggtactttc tccgaccgat gctgtggcgc tctccggaat tgttggcgaa gggcgcatcc
     4201 cgaaaaaaat catgggcatt ttgcagggcg aagcgttgat gaatgacgcc tccggcctgg
     4261 tgtcgttgaa gtttgccgtg gcagtggcga tggggacgat gatcttcacc gtcggcggtg
     4321 cgacggtcga atttatgaaa gtagccattg gtggtattct tgccggtttt gtggtgagct
     4381 ggctgtatgg tcgctcgctg cgattcctca gccgctgggg cggtgatgaa cccgcgacgc
     4441 agatcgttct gctgttcttg ctgccattcg cttcttatct gattgccgaa catattggcg
     4501 tttccggcat cctcgctgcg gttgccgccg ggatgaccat cacccgctcc ggtgtgatgc
     4561 gccgtgcgcc gctggcaatg cgcctgcgcg caaacagcac ctgggcgatg ctggaatttg
     4621 tctttaacgg catggtgttc ctgctgttag gtctgcagct gccgggtatt ctggagacgt
     4681 cgctgatggc ggcagaaatc gaccctaacg tcgaaatctg gatgctgttt accgatatta
     4741 ttctgatata tgcggcgctg atgctggtcc gtttcggctg gctgtggacg atgaaaaagt
     4801 tcagcaaccg cttcctgaag aagaagccga tggagtttgg ttcgtggacc acacgagaaa
     4861 tcctgatcgc gtctttcgcc ggggtgcgtg gggcgatcac tctggccggt gtgctctcta
     4921 tcccgctgct cttgccggat ggtaacgtct tcccggcgcg ctatgagctg gtgttcctgg
     4981 cggctggcgt cattctcttc tcgctgtttg tcggtgtggt gatgttgcct attctgctac
     5041 aacacattga agtcgcggat cattcgcaac aattgaaaga ggaacgtatt gcgcgagcgg
     5101 caacggcaga agtggcgatt gtggcgatcc agaaaatgga ggagcgtctg gcggcggata
     5161 ccgaagagaa tatcgataac cagctgctta cggaggtcag ttctcgcgtc attggtaacc
     5221 tgcgtcgtcg cgccgatgga cgtaacgacg ttgaaagttc cgtgcaggaa gagaaccttg
     5281 agcgtcgctt ccgtctggcg gcattgcgtt ctgaacgtgc tgaactgtac cacctgcgcg
     5341 ccacgcggga gatcagcaac gaaacgctgc aaaaattact gcacgatctc gatttgcttg
     5401 aagcgttgct aattgaggaa aatcagtaag ccgaaagcga cgtaaccagg cccgaggtaa
     5461 tctgaaaaag gtgcaatctt ttgcaccttt aacaggttag ctcgggctaa cattcatgat
     5521 tctaaaacaa ataagttatc tctcttgtaa agtcaattca ttggctgttc ttcgacttaa
     5581 cctcaatctg cgtgttgcat aatatctgca gtaatcttca tagcgactgc attaatatta
     5641 tcctcagttt gtgacatgcc agtatgagag gtacaatctt cttcaaagaa tttctgtagt
     5701 gttaccaggg tatcactatc aatggggata ttttcctctc cacggagtat ggaaatgccc
     5761 attccctgga cgctagcctc atcaaaatta acccgataga gacgggtatt aaacat
//
LOCUS       DAZPEB010000209         5791 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZPEB010000209 DAZPEB010000000
VERSION     DAZPEB010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5791
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.214"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(135..2054)
                     /gene="dhaR"
                     /locus_tag="ACHURJ_003655"
     CDS             complement(135..2054)
                     /gene="dhaR"
                     /locus_tag="ACHURJ_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HFR1282438.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWSVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     HDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLEATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            2282..3391
                     /gene="dhaK"
                     /locus_tag="ACHURJ_003656"
     CDS             2282..3391
                     /gene="dhaK"
                     /locus_tag="ACHURJ_003656"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFR1282439.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQVDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRSFSSLDQTVDEMFDTLLGNGSYHRTLRFWDYQQGSWQEEQQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWDKLGESNVTEQNSNC"
     gene            3363..3995
                     /gene="dhaL"
                     /locus_tag="ACHURJ_003657"
     CDS             3363..3995
                     /gene="dhaL"
                     /locus_tag="ACHURJ_003657"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFR1282440.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            4006..5424
                     /gene="dhaM"
                     /locus_tag="ACHURJ_003658"
     CDS             4006..5424
                     /gene="dhaM"
                     /locus_tag="ACHURJ_003658"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFR1282441.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPKIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLYTVQAKSTL
                     TVEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDYIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            complement(5584..>5791)
                     /locus_tag="ACHURJ_003659"
     CDS             complement(5584..>5791)
                     /locus_tag="ACHURJ_003659"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282442.1"
                     /translation="GINAIFCGREGAGDFINHSPLAKPNKTVFKYQKTLVQDRTSCMI
                     EISFCELSGILEKGIKGINNGEIE"
BASE COUNT         1459 a         1475 c         1426 g         1431 t
ORIGIN      
        1 agcaagtcaa cggaggatat cgcttcagct tttaagtgcg tgatagacaa gcccttaaac
       61 gatgctgaaa aatgttagcg tgcgccggaa tgaaatatag atcactctga tgtattagaa
      121 atcgtttctg tctttcatac ccggcgctta aactgccctg catcaatgcc atgttgcttc
      181 attttccgcc acagcgtagt gcggccgatt ccaagtaaag ccgacatttc ctgaatgcga
      241 ccgcctgtga cctgggctgc gttaataatt gcctcttttt caacttccgc aaatgacaga
      301 ctggtggaaa ggcgggtggc gctgacatca tctgttgcct gctcggtaaa cagatgttcc
      361 ggcaaatcac tgacgcgaat gcgcccatta tcactactca gagcaagatt ctcgatgacg
      421 ctgtaaagtt caaaatcgtt gcctggccat gcacaagaaa ccaggcgagc gagggcatcg
      481 tcatcaattt tcagccgcgt agagaagcgt ttttcaagac tgcgtaattt gttattcacc
      541 agcgccggaa tgctgccacg ccgcatacgc agaggcggga tggtaatttc aaatgcatgc
      601 agcgcgtaat acagctggcg actaaaacga ttttgttcca ccagcattgc gaggtccgcg
      661 gtcgttgttg caatcacttt gacatcaatt ggtattaaac gccgcgcatc cagtcgcgtg
      721 ataacaccct gcttgataac ctgaagcaaa gcagactgta actccaccgc cagatattca
      781 atcttttcaa gaaacagcgt gccgccgtgc gccagttcca gccgactcag acggccattt
      841 tcattgtccg tacgatcgcc accaataaat tcttccgcca gtgccgcatc accatataac
      901 tcacaattga cggcgatata aggacctgca gcacgctcgc tttcattatg aattgcctga
      961 cttagcagtg ccttgcccac gccctcttct ccacaaagca ggacaggaaa gctactgcgc
     1021 gccgcctggc gaccaaaatg aatcaagcgg cgggtttgcg gatcgtcctg tggcatatga
     1081 gcaaaggtat ggctgacttt tcctaattga ctggtcatca actgccgcat ctgttccaca
     1141 ggatggagca acaaaataaa gctggttccc tgcgtttcta ttatcggttt aagggttatc
     1201 accgcatcaa taaactgatg ctgactttca aaggttgctt ctacgtgttt gagcggatgt
     1261 gcctgtttta ttgcttgttg caatacggcg ggtaacgtta agagttcagt gattgcccgt
     1321 ccctgacttg ccgtcgcttc aaggcgcaag acccgcgccg cctgggcatt gataaattgc
     1381 aaattaccct gctcgtccca gctaatcacg ccatcatcca tactttctaa cagggcatta
     1441 agttgattta aatgacggtt agtttcagcg agcaaactgt ccgtcagcag taaatttccg
     1501 acctcgcggg cgattgccaa cgtcaacggc aaatcagctg cggtagtttg ctcaaccgga
     1561 cacgccagcg ctattgttcc cgtcaatcgg cccttgctgt caaacaacgg cgttgcacaa
     1621 aaggcccagt tccagagtgc ctgtttgaaa tgttgatcgg ccatcgtttt cacggcctga
     1681 ccagagatag ccgctaacga tagcgcacaa gtaccaataa ttccctcggc gcaatacgtg
     1741 ccgtcatgga atccaagtgc acttagctgc tgcaaggttt gcggatcgcc attacggctg
     1801 agaatgcagg cggtttcatc gaggataaac agcgcacact ctcgcggtgc catatattcc
     1861 caggcatctt ccagcgcagc ctgcccgagc gtcagcatcg ctttcttacg ccgataaata
     1921 gaagcaaatg tcacgccctg ggcctgatgt ggtacgctcc atgtctcccg tttcatcagc
     1981 ttattgcatc gctcccagga ggttgcaatt aagggagata tgccccgacc atcgttgtta
     2041 aaagcgccac tcatatcccg catcccttat gacaaaaggt gttccggctc gcaattttaa
     2101 gtattaatac acgtaaaagt ttcaatatgg aacattcatt aaagcatatg tttcactatg
     2161 aaacataatt tgggctaatt ttcttttccc ttgcctgatg cacaatggat tcatcggcag
     2221 tcagtggtcg ccgtgtcgtt gaacatcatc catgccctac cgtaattgct ggagcaaaat
     2281 aatgaaaaaa ttgatcaatg atgtgcaaga cgtactggac gaacaactgg caggactggc
     2341 gaaagcgcat ccatcgctga cattgcatca ggatccggtg tatgtcaccc gagctgatgc
     2401 ccctgttgca ggaaaagtcg ccctgctgtc gggtggcggc agcggacacg agccgatgca
     2461 ctgtgggtat atcggtcagg ggatgctttc gggggcctgt ccgggcgaaa ttttcacctc
     2521 accgacgccc gataaaatct ttgaatgcgc catgcaagtt gatggcggcg aaggtgtact
     2581 gttgattatc aaaaattaca ccggcgatat tcttaacttt gaaacagcga ccgagttact
     2641 gcacgatagc ggcgtaaaag tgaccactgt ggtcattgat gacgacgttg cggtaaaaga
     2701 cagtctttat actgccgggc gacgcggcgt tgccaacacc gtattaattg aaaaactcgt
     2761 aggcgcagcg gcggagcgtg gcgactcact ggacgcctgt gcggaactgg ggcgtaagtt
     2821 gaataatcaa ggccactcaa taggtatcgc tctcggtgcc tgtaccgttc ctgccgcggg
     2881 caaaccttct tttaccctgg cggataatga gatggagttt ggcgtcggca ttcatggtga
     2941 gccgggtatt gaccgccgct ccttctcttc ccttgatcaa accgtcgatg aaatgttcga
     3001 caccctgctg ggaaatggct cataccatcg cactttacgt ttctgggatt atcaacaagg
     3061 cagttggcag gaagaacaac aaaccaaaca accgctccag tctggcgatc gggtgattgc
     3121 gctggttaac aatcttggcg caactccgct ttctgagctg tacggcgtct ataaccgcct
     3181 gaccacacgt tgccagcaag cgggattgac tatcgaacgt aatttaattg gcgcgtactg
     3241 tacctcactg gatatgaccg gtttctcaat caccttactg aaagttgatg acgaaacgct
     3301 ggcactctgg gacgccccgg tccacacccc ggcccttaac tgggataaat taggagaaag
     3361 caatgtcact gagcagaact caaattgtta actggctcac tcgttgtggc gatattttca
     3421 gcaccgagag cgagtatctt accggactgg atcgcgaaat tggcgacgct gaccacgggc
     3481 taaatatgaa ccgaggcttt agcaaagtgg tggaaaaact ccctgctatc gcagataaag
     3541 atatcggttt cattctcaag aataccggta tgacgctgct ttccagcgtc ggtggtgcca
     3601 gtggtccgct gttcggtacc ttctttatcc gcgccgcaca ggcgacccag gcacggcaaa
     3661 gcctgacact ggaagagctt tatcagatgt tccgcgatgg cgcggacggc gtaatcagtc
     3721 gcgggaaagc cgaacctggc gataaaacca tgtgtgatgt gtgggtgccg gtggtggaat
     3781 cgttacgtca gtccagcgag caaaatctct ctgttccggt ggcgctcgaa gctgccagta
     3841 gcatcgccga atccgctgca caaagtacga ttacgatgca agcccgcaaa ggccgcgcca
     3901 gttatctcgg tgaacgcagt attggtcacc aggatcccgg cgcgacctcg gtgatgttta
     3961 tgatgcaaat gttggcgtta gccgcaaaag agtaaggaat tggtgatggt aaacctggtc
     4021 atagtttcac atagcagccg actgggagaa ggtgtcggtg aattagcccg tcagatgtta
     4081 atgagtgata gttgtaaaat cgccattgcc gcgggaattg acgatccaca aaatcctatt
     4141 ggtaccgatg ccgtcaaagt gatggaggcc atcgaatctg ttgctgatgc cgaccatgtg
     4201 ctggtcatga tggatatggg tagcgcatta ttgagtgctg aaactgcgct ggaattgctg
     4261 gctcccaaga tcgccgcaaa agtacgtttg tgtgctgcgc cgttggtcga aggtacactg
     4321 gcagcaacgg tcagcgcggc ctcgggagcg gatatcgaca aagttatctt tgacgctatg
     4381 catgcgctgg aagccaaacg tgaacaactg ggtttaccgt cctccgacac tgaaatctct
     4441 gacacatgtc ctgcgtacga tgaagaagcc cgttctctgg cggtggtcat aaaaaaccgt
     4501 aacggcctgc atgtacgtcc ggcctcccgg ctggtttata ccttatcgac atttaatgcc
     4561 gatatgttgc tggaaaaaaa cggcaaatgc gtcacaccag agagtattaa ccagattgcg
     4621 ttactacaag ttcgctataa cgatacgctg cgcctgattg cgaaagggcc agaagctgaa
     4681 gaggcactga tcgctttccg tcagctggct gaagataact ttggtgaaac ggaggaagtc
     4741 gctccaccta ctctgcgtcc cgttccgcct gtttcgggta aagcctttta ttatcaacca
     4801 gttttatata cggtacaggc aaaatcaacc ctgaccgtgg aagaagaaca agatcgatta
     4861 cgccaggcta ttgacttcac gttattagat ctgatgacgt taacagcgaa agcagaagcc
     4921 agcgggcttg actatattgc cgcaatcttt tctggtcacc atacactgtt agatgatccg
     4981 gaactgctgg cggcggcaag cgaactcctt cagcatgaac attgcacggc agaatatgcc
     5041 tggcagcaag ttcttaaaga acttagccag caataccagc aactggatga tgaatatcta
     5101 caagctcgct atattgatgt ggacgatctt ctgcatcgca ccctggtcca cctgacccaa
     5161 acgaaagaag aactcccgca gtttaactcg ccaactattc tactggcgga gaacatttat
     5221 ccttccacag tactgcaact ggatccggcg gttgtaaaag gtatctgcct tagcgccgga
     5281 agtccggtat cccacagcgc cctaatcgcc cgtgaactgg ggattggctg gatttgccag
     5341 cagggtgaga aactgtatgc gatacaacca gaagaaacgc taacgctgga cgttaaaacg
     5401 caacgtttca accgtcaggg ttaagccact gttcggatgg catcgttctg atgtcatccg
     5461 gcaatttact cagcactaat ggtttttaac tacctgttat atttttcacc aatttatgat
     5521 gatttttctt cgctaatcac gaacccaacg ccgctggctg gctaataaac agacgttatt
     5581 tgatcactct atttcgccgt tatttatccc tttaattccc ttttctaaaa tgcctgacag
     5641 ttcgcagaat gagatttcga tcatgcagct agtgcgatcc tgaactaagg ttttctgata
     5701 cttgaatacc gttttattcg gtttcgccaa aggagaatga ttgatgaaat cccccgcacc
     5761 ttctcgcccg caaaaaatgg cgttaattcc a
//
LOCUS       DAZPEB010000210         5765 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZPEB010000210 DAZPEB010000000
VERSION     DAZPEB010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5765
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.215"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(559..864)
                     /locus_tag="ACHURJ_003660"
     CDS             complement(559..864)
                     /locus_tag="ACHURJ_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008325193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282443.1"
                     /translation="MGSKIAGVVFLYFAVWGMASVIELQYWRNADTYPVWLTKHLRIS
                     PENNDKSLIRIEMETFDKVCGDKRGLLAVTQKQNGMFMRCDDSPGFFSWFSGVYKVN"
     gene            complement(918..1169)
                     /locus_tag="ACHURJ_003661"
     CDS             complement(918..1169)
                     /locus_tag="ACHURJ_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004182043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFR1282444.1"
                     /translation="MTNIILCDVTASVSELKNDPVATASARGGYPVEIIDRNRPVFYC
                     VPAALYEQMLDELDEKDLVQTITERQNQPLREVDLNQYL"
     gene            complement(1248..2519)
                     /locus_tag="ACHURJ_003662"
     CDS             complement(1248..2519)
                     /locus_tag="ACHURJ_003662"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0019985 - translesion synthesis [Evidence
                     IEA];
                     GO_process: GO:0042276 - error-prone translesion synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFR1282445.1"
                     /translation="MFALADVNSFYASCEKVFRPDLRGKPVVVLSNNDGCVIARSAEA
                     KRLGIKMGTPWFQLKEAQFPEKLYVFSSNYELYASLSNRVVALLEELSPRVEQYSIDE
                     CFLDARGISHCMDLEDFGRQLRGHVLSGTGLTIGVGFGATKTLAKSAQWASKEWPQFS
                     GVLALSPENPRRTAKLLSLQPVEEIWGVGNRIAKKLHVMGITTALQLSLTNPTFIRKN
                     FNVVLERTVRELNGESCISLEEAPPPKQQIVCSRSFGQRITTYGEMRQAVCQYAERAA
                     EKLRGERQYCRHISTFIKTSPFAVNEPYYGNVATEKLNTPTRDTRDIIAAAVRSLDRI
                     WLDGHRYAKAGIMLNDFSPNGVAQLNLFDDVQPRPHSDALMKVLDGINHSGLGKVWFA
                     GRGIAPDWQMKREMLSPAYTTRWKELPVARF"
     gene            complement(2519..2944)
                     /gene="umuD"
                     /locus_tag="ACHURJ_003663"
     CDS             complement(2519..2944)
                     /gene="umuD"
                     /locus_tag="ACHURJ_003663"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFR1282446.1"
                     /translation="MKFIPAVSEASIVKIPLFTERCPAGFPSPAANYTESELDLNEYC
                     IHRRHSTYFVRAIGNSMTDIGLYSGDLMVVDKAEQPRHGDIVIAEIEGEFTVKRLLLT
                     PRPALQAMNPDFPSLYPDPETLQIFGVVTAFIHKTRRAD"
     gene            3045..4265
                     /locus_tag="ACHURJ_003664"
                     /pseudo
     CDS             3045..4265
                     /locus_tag="ACHURJ_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISKox3 family transposase"
     gene            4720..5685
                     /locus_tag="ACHURJ_003665"
     CDS             4720..5685
                     /locus_tag="ACHURJ_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zincin-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HFR1282447.1"
                     /translation="MTMNLHTQTTAPKASQATSVSPLEQSGSSKTKFSRGKSKARAGA
                     VKTDLYQTVTDSIIAALESGVKPWVCPWVRNGAAVGLPANFSTGTAYSGINIMLLWCS
                     AAKQGFQDERWLTYKQAQELGGQVRKGEHGTTAIFYKTLEKEDEDGEIEKIPMLKAFT
                     VFNVEQIDGLSIESVPQPVAGFDPLPQAEALMIRSGAKITEQGVKAFYRPATDEIVLP
                     ERFRFADAANFYATGLHELVHWTGAASRLNREKGNKFGSEAYAFEELIAELGSAFLLA
                     DLGITGEVQHESYIASWLKALKGDKRYIFKAAAAASKAHRWLMDC"
BASE COUNT         1369 a         1530 c         1575 g         1291 t
ORIGIN      
        1 ctgggaccac ggtgccacct gtatcgccgg tcaggttatc tgcctgggac cacggtccca
       61 cctgcatcgt cggcacatta ttagttaggg acaacggttc aatcaatgcc tgtcacgaac
      121 aggtattggc ttgtcgccag caacccgttg agcggagtaa tcacgcagca ggacggctgt
      181 tacaggcgat ggtgaaagct ctgttgggat ctgaacgaag catgttgagt gaaatttgca
      241 aaatttcgca gagtatgcgg cttcatcttt aaatttccgc cagcattttt aaagccagct
      301 accgacgctg cgcgttggcc ggcccttcgt ccggcgggta accagtaccg gccgatctta
      361 cgaccctgga tgaacatcat cccgtcagca gcgtctccac tgtgatgtcg tttttcacac
      421 tgtccgatca caaaatagaa cgggcaggga caacatatgg taggaaaagc agctgccaga
      481 gacacaatat attgattacc cacagtaacg ggggataacc atgtgggtaa catattgaaa
      541 gtgcaacacg ttatctgatt aattgacttt gtaaacgccg ctgaaccatg aaaaaaaacc
      601 gggggaatca tcacagcgca taaacatacc gttttgtttt tgggtgacgg caaggaggcc
      661 ccttttatca ccacatacct tgtcaaaggt ttccatttct atccttatca ggctcttgtc
      721 gttattttca ggagagatcc ggagatgttt tgtcagccag acaggatagg tatcagcgtt
      781 acgccagtac tgcaattcaa tcactgaagc cattccccag acagcgaagt ataagaaaac
      841 cacacctgcg attttgctac ccatattgtt ccttaacatt ggcttaatac attccttgct
      901 cctcgcaccg gagcacatca cagatactga ttgagatcca cttcacgcag cggctggttt
      961 tgacgttctg ttatcgtttg caccaggtct ttttcgtcca gctcatcgag catttgttca
     1021 tacaaagccg cgggcacaca ataaaaaaca gggcggttac ggtcaattat ctcgaccggg
     1081 tagccgccgc gggcactggc ggtggccacc ggatcgtttt taagttcgct gacgctggca
     1141 gtcacatcac acagaatgat atttgtcatg gttcccccct gagacaagtc acctgtatga
     1201 tcggtaaagt aagctctggc ggcaagtccc ttctgtcatt tcaacggtca gaaacgggca
     1261 accggcagct ccttccagcg tgtggtatat gccggtgaca gcatctcccg tttcatctgc
     1321 cagtccggcg caataccccg tccggcaaac cagactttcc cgagtccgga atgattaatg
     1381 ccgtcgagta ccttcatcag cgcatcgctg tgcggccgtg gctgcacgtc atcgaacaga
     1441 ttcagctgcg cgacgccgtt cggactgaaa tcattgagca ttatccccgc ttttgcgtag
     1501 cggtggccat cgagccatat cctgtccaga gacctgaccg ctgccgcgat aatgtcccgg
     1561 gtgtcacggg taggggtgtt cagtttttcc gtggccacgt taccgtaata cggctcatta
     1621 accgcaaaag gggatgtctt gatgaaggtg ctgatatgcc ggcagtactg tcgctcaccg
     1681 cgcaactttt ccgccgcgcg ctcggcatac tggcagacgg cctggcgcat ttctccgtag
     1741 gtggtgatcc gctgcccgaa gctgcgactg cagacaatct gctgtttcgg cggcggcgct
     1801 tcctccagcg aaatgcaact ctcgccgttc agctcacgta ccgtgcgctc cagcactacg
     1861 ttgaagtttt tccggataaa tgtagggttc gtcagcgaca gctgcagggc ggtggtgatc
     1921 cccataacgt gcagcttttt cgctatccgg ttccccacgc cccagatttc ttccaccggc
     1981 tgcaggctga gtaattttgc cgttctgcgt ggattttcgg gcgacagcgc cagcacccca
     2041 ctgaattgtg gccattcctt tgacgcccac tgcgccgatt tggccagtgt ttttgtggcg
     2101 ccgaagccga caccgatcgt cagtccggtt ccactgagaa catgcccacg cagctgcctg
     2161 ccaaaatcct ccagatccat acagtggcta attccccggg catccaggaa acattcgtca
     2221 attgagtatt gttcgacgcg gggcgacagc tcttccagca gggcgaccac acggttactc
     2281 aggctcgcgt agagctcgta gttgctggaa aaaacgtaca gtttttcagg aaactgcgcc
     2341 tctttgagct ggaaccaggg ggttcccatc tttataccca gccgtttggc ttcagcactc
     2401 cgggcaataa cgcagccatc gttgttactg agaacgacga caggtttgcc gcgcaaatcc
     2461 ggcctgaaaa ccttctcgca actggcgtag aaggagttga cgtctgccag ggcgaacatc
     2521 agtctgccct ccgggtttta tgaatgaagg ccgtcaccac gccgaaaatt tgcagagttt
     2581 ccggatccgg gtacagggaa gggaagtccg ggttcatcgc ctgcagggct ggccgcggcg
     2641 tcagaagcag gcgttttacc gtaaattcac cctcaatttc cgcgataaca atatccccgt
     2701 gccgcggctg ttctgcttta tccacgacca tcagatcgcc ggaatagagg ccaatatcag
     2761 tcattgagtt gccaatcgct ctgacaaaat aggtggagtg gcgccggtga atgcagtact
     2821 cattcagatc cagttcagac tcagtatagt ttgctgccgg tgacggaaaa cccgcagggc
     2881 atcgctctgt gaacaaagga atttttacta tgctggcttc tgatactgca ggaatgaact
     2941 tcatcgtttt ttccctcgct taaaactgta tatgcatggg tcttcccttg ttgtggtggc
     3001 tgaaggcatg ataatggtgt atttaatcgc cagaggtcac cgccatggac gaaaagtccc
     3061 tctacgctca tattctcaac ctgtccgatc cgtggcaggt aaagtccctt tctctcgatg
     3121 aaaatgccgg ttctgttact gtcactattg agatcgctga aaacacccgg ctagcctgtc
     3181 cgacctgcgg taaatcctgt tctgttcacg atcaccgtca tcgtaaatgg cgccatcttg
     3241 atacctgcca gttcaccact attgttgaag ccgatgttcc acgaattatg tgtccggagc
     3301 atggctgcct gacgttgcct gttccgtggg ctggccccgg aagccggtat acgttgctat
     3361 tcgaatcgtt cgttctctca tggctgaaaa tcagcaccgt tgatgctgtc aggaagcaac
     3421 ttaagctcag ttggaatgcg gttgacggca ttatgacccg ggcagttaag cgaggtcttg
     3481 cccggataaa aaagccatta tccgcccgtc atatgaatgt ggatgaggtc gcctttaaaa
     3541 aaggacatcg ttacataacg gtgatctccg atcgcgatgg tcgggcgctg gccttaacgg
     3601 atgatcgcgg cacagagagt cttgccggct atcttcgcac gctcactgat gggcagttgc
     3661 tggctatcaa aacgctctca atggacatga acgcgggcta tataagagca gcgcgtatcc
     3721 acttacccag tgcggttgag aaaatcgcct ttgaccgctt ccatgtggcg aagcaactgg
     3781 gcgaggtagt tgataaaacc cgtcagaatg aacatccgca cctccctgtt gaaagccgac
     3841 accaggcaaa aggaacccgc ttcctgtggc agtacagcga taagtggatg accgaatccc
     3901 ggcaggaaaa gctgatgtgg ctgcgtgcac agatgaagct gacgagccag tgctgggcgc
     3961 tgaaagagct ggcaaaggat atctggaaca ggccatggag cgaggaaaga cggagtgact
     4021 ggcagagatg gttggcgctg gcggctaaca gtgacgttcc catgatgaaa aatgccgcga
     4081 aaacgatagg aaaaaggctg taggggatcc tgaatgcgat gcgacacagt gtctcaaacg
     4141 gaaatgcgga ggcacttaac agcaagatca ggctgctgag gataaaagcc aggggatacc
     4201 gaaaccggga gcgctttaaa ctgggggtga tgttccacta cgaaaagctg aatatggcgt
     4261 tctgagcctt cccaccatga tcggggaaga cccatatgca tacagtataa ggggcggtga
     4321 aatttctgca agtgatgggg ggatagccgg tcagaacgtc cccgaaaaca aaccgccccc
     4381 ggtttcagga agggcgaact gaccggcaga ggcagtgtaa caccgcatgt cacaaaggca
     4441 gcactgacgt cgtcagtgct ttttttaccc gtcctcccgg acgctgaagg gcagtttcac
     4501 ccgtgcagct aaaggggcgg taagtccggg tgtgcacatc gtcattttct cctgtgatgc
     4561 ctttctcctg cgtatcagct tccggtaatc gtcccccgtc acaacctgga cagcaagggt
     4621 aaatggcacg ctgcgcgccc ttgctgacca gaacgactcc gggcaggccg aaccgtcagg
     4681 cgatacgaag acgaaattca cactgacgga gaaacagcca tgacgatgaa cctgcacact
     4741 cagacaaccg cccctaaagc ttcacaggcg accagcgtat ccccgctgga gcagtcaggc
     4801 tcctcaaaaa cgaaattttc caggggcaaa agcaaagccc gtgctggcgc agtcaaaaca
     4861 gacctttacc agacagtaac ggacagcatc attgcagccc tggaaagcgg cgttaagccg
     4921 tgggtgtgtc cgtgggttcg caacggcgca gcggtgggat taccggccaa cttcagcacc
     4981 ggcacggcat acagcggaat taatatcatg ctgctgtggt gtagtgcggc aaaacagggt
     5041 tttcaggacg agcgctggct gacctacaaa caggcgcagg agctgggcgg ccaggttcgt
     5101 aaaggtgaac acggcacgac ggccatcttt tacaagacgc tggagaaaga ggacgaggac
     5161 ggggaaatcg aaaaaatccc gatgctgaaa gcctttaccg tgtttaacgt ggagcaaatc
     5221 gacggcctga gcatcgagag cgtgccgcag ccggttgccg gtttcgatcc gctgccgcag
     5281 gcggaagcac tcatgatccg gagcggggca aaaatcaccg agcagggcgt aaaggcgttc
     5341 tatcgtccgg ccaccgatga aatcgtcctg ccggagcggt tccgcttcgc ggacgcggcg
     5401 aacttctacg ccaccggcct gcatgagctg gttcactgga cgggtgcagc gtcccgcctc
     5461 aatcgtgaaa aaggcaataa gtttggctct gaggcgtatg cgtttgagga actgatcgcc
     5521 gagctgggca gcgcgtttct gttggcggat ttggggatta cgggagaggt gcagcatgaa
     5581 agctatatcg cgtcctggct gaaagcgctg aagggagaca agcgctacat ctttaaagcg
     5641 gcggcagcgg cctcaaaagc gcaccgctgg ctgatggact gctgacagct ggagccctgc
     5701 ggtgcagggc ttcagaattt ttgaaagaga gagaatcagg gagggaatta tataagtcac
     5761 tgttt
//
LOCUS       DAZPEB010000211         5728 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZPEB010000211 DAZPEB010000000
VERSION     DAZPEB010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5728
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.216"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(85..621)
                     /gene="ssb1"
                     /locus_tag="ACHURJ_003666"
     CDS             complement(85..621)
                     /gene="ssb1"
                     /locus_tag="ACHURJ_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFR1282448.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            875..3697
                     /gene="uvrA"
                     /locus_tag="ACHURJ_003667"
     CDS             875..3697
                     /gene="uvrA"
                     /locus_tag="ACHURJ_003667"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFR1282449.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3732..4088)
                     /locus_tag="ACHURJ_003668"
     CDS             complement(3732..4088)
                     /locus_tag="ACHURJ_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFR1282450.1"
                     /translation="MTISELLQYCMAKLGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4092..4508)
                     /locus_tag="ACHURJ_003669"
     CDS             complement(4092..4508)
                     /locus_tag="ACHURJ_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFR1282451.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMQGTSLVL
                     PLHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4619..5332)
                     /gene="aphA"
                     /locus_tag="ACHURJ_003670"
     CDS             complement(4619..5332)
                     /gene="aphA"
                     /locus_tag="ACHURJ_003670"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFR1282452.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         1380 a         1480 c         1477 g         1391 t
ORIGIN      
        1 cataacggag gcttcttttt tatggctggc agatgcttta atcatccacc ttaaaacaat
       61 ataacctatt gttttaatga caaatcagaa cggaatgtca tcatcaaagt ccatcggcgg
      121 ctcgttagac ggcgctgccg gagcggactg ctgcgggcga gactgcgcgc cgccgctgaa
      181 ctgattgcca ccctgcggct gctgaggctg accccaaccg ccctgcggct gaccaccacc
      241 gatattacca cctgccggag cgccaccacc ctgacgacca cccagcatct gcatggtgcc
      301 gccaacgttc accacgactt ctgtggtgta gcgatcctga ccggattgat cggtccattt
      361 acgggtacgc agctgacctt cgatataaac ctgagaacct ttacgcagat attcgctggc
      421 cacttctgcc agtttgccga acagcacaac gcggtgccat tcagtctgtt ctttcatctc
      481 gccggtcgct ttatcacgcc aggattcgga agtagccagc gtaatgttgg caactgcgcc
      541 accatttggc atgtagcgta cttccgggtc ctgacccaga ttaccaacga gaataacctt
      601 gtttacgcct ctgctggcca tgttcgtgtc tcctgaaaaa aatcgttctg aataagtgta
      661 aacgcgcgat tgtaccatta ccaatagcgc ttttactatg ttgtgacctc ggttccggga
      721 aacaaacctg gccagacatt gttacacaac actccggata atgcattcca atactgtata
      781 ttcattcagg tcaatttgtg tcataattaa ccgtttgtga tcgccggtag caccatgcca
      841 ccgggcaaaa aagcgtttaa tccgggaaag gtgaatggat aagatcgaag ttcggggcgc
      901 ccgcacccat aatctcaaaa acatcaacct cgttatcccc cgcgacaagc tcattgtcgt
      961 gaccgggctt tcgggttctg gcaaatcctc gctcgctttc gacaccttat atgccgaagg
     1021 gcagcgccgt tacgttgaat ccctttccgc ctacgcgcgg cagtttctgt cactgatgga
     1081 aaagccggac gtcgatcata ttgaggggct ttctcctgcc atctcaattg agcagaaatc
     1141 gacgtctcat aacccgcgtt ctacggtggg gacaatcacc gaaatccacg actatttgcg
     1201 tttgttgttc gcccgcgtcg gcgaaccgcg ctgtccggac cacgacgtcc cgctggcggc
     1261 gcaaaccgtc agccagatgg tggataacgt gctgtcgcag ccggaaggca agcgtctgat
     1321 gctactcgcg ccaatcatta aagaacgcaa aggcgaacac accaaaacgc tggagaacct
     1381 ggcaagccag ggttacatcc gtgctcgtat tgatggcgaa gtctgcgatc tttccgatcc
     1441 gccgaaactg gaactgcaaa agaaacacac tattgaagtg gtggttgatc gcttcaaggt
     1501 gcgtgacgat cttacccaac gtcttgcgga gtcgtttgaa accgcgctgg agctttccgg
     1561 tggtacagcg gtagtggcgg atatggacga cccgaaagcg gaagagctgc tgttctccgc
     1621 caacttcgcc tgcccaattt gcggctacag tatgcgtgaa ctggagccac gactgttttc
     1681 gtttaacaac ccggcaggtg cctgcccaac ctgtgacggc cttggcgtac agcaatattt
     1741 cgatcctgac cgcgtgatcc aaaaccccga gctgtcactg gctggcggtg cgatccgtgg
     1801 ctgggatcgc cgcaacttct attacttcca gatgctgaaa tcgctggcag atcactataa
     1861 gttcgacgtc gaagcgccgt ggggcagcct gagcgcgaac gtgcataaag tggtgttgta
     1921 cggttctggc aaagaaaaca ttgaattcaa atacatgaac gatcgtggcg atacctccat
     1981 ccgtcgtcat ccgttcgaag gcgtgctgca caatatggag cgccgttata aagagacaga
     2041 atccagtgcg gtacgtgaag aattagccaa gtttatcagc aatcgcccat gcgccagctg
     2101 cgaagggacg cgtctgcgtc gggaagcgcg ccacgtttat gtcgagaata cgccgctgcc
     2161 tgctatctcc gacatgagca tcggtcatgc gatggaattc ttcaacaatc tcaaactagc
     2221 tggtcagcgg gcgaagattg cagaaaaaat ccttaaagag atcggcgatc gtctgaaatt
     2281 cctcgttaac gtcggcctga attacctgac actttcccgc tcggcagaaa cactttccgg
     2341 cggtgaagcc cagcgtatcc gtctggcgag ccagattggt gcgggcctgg ttggcgttat
     2401 gtatgtactg gacgagccgt ctatcggcct gcaccagcgc gataacgagc gcctgttggg
     2461 tacgcttatc catctgcgcg atctcggtaa taccgtgatt gtggtggagc atgacgaaga
     2521 cgcaattcgc gccgctgacc atgtgatcga tatcggtcct ggtgcgggtg ttcacggcgg
     2581 tgaagtggtc gcagaaggtc cgctggaagc gatcatggcg gtgcctgaat cgttgaccgg
     2641 gcagtacatg agcggtaaac gcaagattga agtgccgaag aaacgcgttc cggcgaatcc
     2701 agaaaaagtg ctgaagctga caggtgcacg cggcaacaac ctgaaggacg tgacgctcac
     2761 gctgccagtc ggtctgttta cctgcatcac aggggtttca ggttccggta aatcgacgct
     2821 gattaacgac acactgttcc cgattgccca acgccagttg aatggtgcga ccatcgccga
     2881 accggcaccg tatcgcgata ttcaggggct ggagcatttc gataaagtga tcgatatcga
     2941 ccaaagccca attggtcgta ctccacgttc taacccggcg acctataccg gcgtgtttac
     3001 acctgtgcgc gaactgtttg cgggcgtacc ggaatcccgt gcgcgtggtt atacgccagg
     3061 acgtttcagc tttaacgtcc gtggcgggcg ctgcgaagcc tgtcagggcg acggtgtgat
     3121 caaagtggag atgcacttcc tgccggatat ctacgtgccg tgcgatcagt gcaaaggtaa
     3181 acgctataac cgtgaaacgc tggaaattaa gtacaaaggc aaaaccatcc acgaagtgct
     3241 ggatatgacc atcgaagagg cgcgtgagtt ctttgatgcg gtgccagctc tggcgcgtaa
     3301 gctgcaaacg ttgatggacg ttggtctgac gtacattcgc ctggggcagt ccgcaaccac
     3361 actttctggt ggtgaagccc agcgcgtgaa gctggcgcgt gagctgtcaa aacgcggcac
     3421 cgggcagacg ctgtatattc tcgacgagcc gaccaccggt ctgcacttcg ccgatattca
     3481 gcaactcctc gacgtgctgc ataaactgcg cgatcagggc aataccattg tggtgattga
     3541 gcacaatctc gacgtgatca aaaccgctga ctggattgtc gacctgggac cagaaggcgg
     3601 cagtggcggc ggcgaaatcc tcgtctccgg tacgccagaa accgtcgcgg agtgcgaagc
     3661 ttcgcatacg gcacgcttcc tcaagccgat gctgtaatcg ttaaggccgc tttctgagcg
     3721 gccttttcct ttcagagttg taccagcaat ttacgctttt cttccggtag taaattcact
     3781 gcctgctgat aagacgcatc caccagataa tagatttgcg aatccggcag cgaaccgtcg
     3841 agatacacgg tgctccagtg cgctttattc agatggcggc taggacgcac atcgctgtgc
     3901 tgctgacgta gcagctccgc cagctccggg ctggttttca gcgaaacagc tgggcgattt
     3961 tctacttctt tcaccatcgc aaacagtaca tcttccactt tgatctgcgt cgctttccag
     4021 tcgttatgca cgctctgttc tgcgcctagt tttgccatgc aatattgtag caactccgaa
     4081 atggtcattt tttactcccc ttgtagtgtc gcgatgatgc gacgcgatcc gccgtggatg
     4141 cgatgttccc ccagccagat gccttgccag gtgccggtct gaatacgccc tttatgtaac
     4201 ggcaatacaa gcgatgttcc ctgcattgag gatttgatat gagaaggcat atcgtctgct
     4261 ccctcatagt catgctcata atttccgttg tcgggaacgg tgcggaggaa aaaacgctcc
     4321 atgtcgtggc gtacggtggg atcgcagttc tcattaagtg tcagagaggc ggaggtatgt
     4381 tgcagcaaca gatgcagtaa gccgatgtta acgcgcggca tatcagccag ctgattcaga
     4441 atttcatccg ttaccagatg aaacccacga gatttggcgc taagcgtgag cgtcttttga
     4501 taccacatgt gctgctcctt gataaaactc tcttaatcag tttgcagcaa gacagcgaaa
     4561 ggataaaggt gtgattaaaa aaacagcatt cgggagagca tcacgctctc ccgctctgtc
     4621 agtattctga attgacgatc acctcttcac caaacgcacc cgcttgtggc aagggtttgt
     4681 aggtagagtt ggaggcgcgc agaatgcgga taccacgagc gccgacatcg cgtgcggcgg
     4741 taatatcatt atcagaatcg ccataaaaaa ttcggatatt tttatcctgc agccattgcg
     4801 attttgtatt ttgccctggt ttatcgcccg caaagatcac cggattcatg ttggtggcag
     4861 gaatatgaaa attatccgcc agcgtttttg aaaccgtttc tgttttcgtc ggactacgac
     4921 cagtcacaaa gaagatcgcg tcaccgcggc gtacatgcat atcaatcagc tggcgagcga
     4981 cctcttttgg aatgctgaat tcatcccagc cattgttcat tttttcccag aacacaggat
     5041 ttttcagata atcttcgctt tctggcgaga aggttttttt gccgcgccag aagcccgggc
     5101 tggaaaaaag taccgtgtca tcgatatcaa accccaccgc cattggcgga cgccctgcga
     5161 ggctattttc aatttgtgcg accgaaaccc aatgaattgg tgcctgttca gcaagcctgg
     5221 caacgttagt accagggtta agcggtgaag gagatgaggc cagggcaaca gcggaactgt
     5281 ttagagcgaa caataagcaa acggcactga ttgcctgtgt gatcttgcgc atatttttcc
     5341 ctaaatagtc agtatgttga aactttttat tgtgagattt gttgcaaaaa ccatctgacc
     5401 ataacgccag ctgcagggga aaggaaggat tatctgcggt ttttgtgatg aaaagcagga
     5461 tgattatatt taacccagta aatacgttcc tgtctttttt gtataaaaaa tgtttattac
     5521 tcgatgtaag gatacattaa ttttacgttt attatgatga atcctctgca aatgagcgaa
     5581 atttgataga acatttatct gctgtacgat ttaatgaact taatgataca gcattacaaa
     5641 aacaatcgaa gtttataaag atgattcctg attgaccaga accctatcct acaatggtga
     5701 ttaattttat tactcgcgat ggagatga
//
LOCUS       DAZPEB010000212         5622 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZPEB010000212 DAZPEB010000000
VERSION     DAZPEB010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5622
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.217"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            192..2602
                     /gene="ynfE"
                     /locus_tag="ACHURJ_003671"
                     /pseudo
     CDS             192..2602
                     /gene="ynfE"
                     /locus_tag="ACHURJ_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfE"
     gene            2663..5086
                     /gene="ynfF"
                     /locus_tag="ACHURJ_003672"
     CDS             2663..5086
                     /gene="ynfF"
                     /locus_tag="ACHURJ_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HFR1282453.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKCGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMLDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            5097..5622
                     /locus_tag="ACHURJ_003673"
                     /pseudo
     CDS             5097..5622
                     /locus_tag="ACHURJ_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
BASE COUNT         1525 a         1424 c         1414 g         1259 t
ORIGIN      
        1 cgactgaacg ccggatatga cacatccggc gatttgaaca tacaacataa tcccacctta
       61 ttactcatac ccttctattg atgtggatta ataattctta acccaaaatg ggtagccccc
      121 cctctattgt tagcgcgcta aatattcaat acataaacat ttatataacg ataaagaaca
      181 gggagtgagt tatgtccaaa aatgaccgaa tggtgggcat cagccgcaga acactcgtta
      241 aatcaaccgc gataggttct ctggcgctgg ctgcaggcgg tttttcttta ccgttcaccc
      301 tgcgcagtgc agcggcaacg gtacaacagg ccagcgaaaa agtgatctgg ggtgcctgtt
      361 ccgtcaactg tggtagccgc tgtgcacttc gtctacatgt taaagataat gaagtgacct
      421 gggtggaaac tgacaatacc ggcagcgatg agtacggcaa ccatcaggta cgcgcctgtt
      481 tgcgcggtcg ctccatccgc cggcgtatta atcatcccga tcgcttgaat tacccaatga
      541 aacgcgtggg taaacgcggt gaaggcaaat tcgaacggat tagctgggat gaagccctgg
      601 atactatcgc cagtagcctg aaaaaaaccg tcgaacaata tggcaatgag gctgtatata
      661 ttcagtactc ttcggggatt gttggcggca atatgacccg ctcttcgcca tcagcctcgg
      721 cggtcaaacg cctgatgaac tgctacggcg gttcactcaa tcagtatggc tcctacagca
      781 ccgcacaaat ttcctgtgcc atgccctaca cctacggcag taatgatggc aacagcacca
      841 cggatattga aaacagcaag ctggtcgtga tgtttggtaa caacccggca gaaacccgca
      901 tgagcggcgg tggcatcact tatcttcttg aaaaagcgcg cgagaaatcg aacgccaaaa
      961 tgattgttat cgatccgcga tataccgata cggctgctgg tcgtgaagat gaatggcttc
     1021 ctattcgccc gggcaccgat gccgcgctgg ttgcgggtat tgcctgggta ttgattaacg
     1081 aaaatctcgt tgatcaacct tttctcgata aatactgcgt cggctatgac gaaaaaacct
     1141 tacccgcaga tgcaccaaaa aatggtcact ataaagccta tattcttggt gaaggtgatg
     1201 ataatacagc gaaaacgccg cagtgggctt cgcaaattac cggtatcccg gtggaccgta
     1261 tcatcaaact ggcgcgtgaa attggcacag caaaacccgc ttatatctgc cagggctggg
     1321 ggccgcaacg ccaggcaaac ggcgaactga ctgcacgcgc tattgctatg ttgcctattt
     1381 tgacgggcaa tgtcggcatc agcggcggaa atagtggcgc gcgtgaatcg acctatacca
     1441 ttaccataga acgcctgccg gtgctggata atccggtcaa aacgtcaatc tcctgcttca
     1501 gctggacaga tgctatcgat catggtccgc aaatgacggc aatccgcgac ggcgtccgcg
     1561 gcaaagataa actggatgtg cccattaagt tcatctggaa ctacgcggga aataccctcg
     1621 ttaatcagca ttctgacatc aacaaaacgc atgaaattct gcaggacgaa tcgaaatgcg
     1681 aaatgattgt ggtcatcgaa aactttatga cctcatcggc aaactgccag acctgatgac
     1741 cgttgagcag gaagatatta ttcctaacga ctacgccggt aacatgggat atctcatttt
     1801 cctccagcct gtcaccagcg aaaaattcga acgcaaaccg atttactgga tcctgagtga
     1861 agtcgcgaaa cgtcttggac ctgacgtcta tcaaaagttc acagaaggtc gcacgcagga
     1921 acaatggtta caacatctgt acgccaaaat gcttgccaaa gatccggcgt taccgtctta
     1981 cgacgaactg aaaaaaatgg gtatctataa gcgtaaagat cccaatggcc attttgtcgc
     2041 ctacaaagca tttcgtgacg accccgaggc aaatccactt aaaacgcctt ccggtaagat
     2101 tgaaatttat tccagcaggc tggcggaaat tgcccgtacc tgggaactgg aaaaagatga
     2161 agtgataagc ccattgccag tttatgcctc aacctttgaa ggctggaact cccctgaacg
     2221 tagaaccttc ccactgcaac tgtttggttt ccattacaaa tcccgtactc actcgaccta
     2281 cggcaatatt gatctcctga aggctgcctg ccgtcaggag gtgtggatca accctataga
     2341 tgcgcagaaa cgtgggattg ccaacggcga tatggtgcgg gtgtttaacc atcgtggcga
     2401 agttcggcta ccagccaaag taacaccacg tattctccct ggagttagcg ctatgggcca
     2461 gggagcctgg cacgaggcca atatgtctgg cgataaaatc gaccatggcg gctgtgtgaa
     2521 tacgctaacc actctgcgcc cttcaccact ggcgaaggga aacccgcagc acactaatct
     2581 ggtcgagatc gaaaaaatat aacccacgac aaccataaat tctggcatga cattttgttt
     2641 gaaaagcaat aagtgagtaa tgatgaaaat ccataccaca gaggcgctca tgaaggctga
     2701 aatcagccgt agaagtctga tgaaaacctc cgcacttggc agtcttgcgc tggcaagcag
     2761 tgctttcact ctgccatttt cccaaatggt ccgggcggca gaggctccgg tagaagagaa
     2821 agcggtctgg agttcctgca ccgttaactg cgggagccgc tgtctgttac gtttgcatgt
     2881 gaaagatgac accgtgtact gggtggagtc tgatacgaca ggtgacgacg tctacggtaa
     2941 tcatcaggtt cgagcgtgtt tacgcgggcg ctctatccgc cgacggatga atcatcctga
     3001 taggttgaaa tatcccatga agcgcgtcgg caagtgcggt gaaggtaaat ttgaacggat
     3061 aagttgggac gaagccctgg ataccatcag tgataatctt cggcggatcc tgaaagatta
     3121 cggcaatgag gctgtacatg tcctgtacgg aacaggcgta gatggcggaa acatcaccaa
     3181 ctcaaacgtc ccgtaccgtc tgatgaactc ttgcggtggt tttctcagtc gctatggcag
     3241 ctacagtacc gcacagatca gtgccgcaat gagttatatg ttcggtgcca atgatggcaa
     3301 cagcccggat gatatcgcca atacgaaact ggtcgttatg ttcggaaata acccggcaga
     3361 aacgcggatg agcggcggtg gtgtcactta ctacgtcgag caagcccgcg aacgttcaaa
     3421 cgcacgcatg atcgtcatcg atccacgtta taacgacact gctgccgggc gtgaagatga
     3481 atggctgcct attcgccctg gcaccgatgg cgcactggcc tgtgcgattg cctgggtact
     3541 gattactgaa aacatggtcg atcagccatt tctcgacaaa tattgtgttg gttacgatga
     3601 aaaaacgctg cccgccaacg caccacgtaa cgcgcattat aaagcctata ttctgggcga
     3661 agggcctgac ggcatagcta aaacgccgga atgggcagca aaaatcacca gcatcccggc
     3721 agaaaaaatt atccagttgg cgcgagagat cggttcagca aaacctgctt atatttgtca
     3781 gggttggggg ccacaacgac attccaacgg cgagcaaacg tcccgcgcta ttgccatgct
     3841 ttccgttctc accggtaacg ttggcataaa cggcggcaac tcaggtgtac gcgaaggtag
     3901 ctgggatctg ggggtagaat ggttcccgat gcttgagaat cctgttaaaa cgcagatttc
     3961 cgtctttaca tggacagatg ccatcgacca tggtacggaa atgaccgcga cccgtgatgg
     4021 tgttcgtgga aaagaaaaac tggatgtccc catcaagttt ttatggtgct acgccagtaa
     4081 cacattgatc aatcaacatg gcgacatcaa tcacacccat gaggtgcttc aggatgacag
     4141 caagtgcgag atgattgttg gcattgacca cttcatgacg gcctcggcta agtattgcga
     4201 tatcctgttg cccgacctga tgccaacaga gcaagaagac cttatctctc atgaatctgc
     4261 agggaatatg ggctatgtga tcctcgccca acccgcaacc tcagcaaaat ttgaacgcaa
     4321 acccatctac tggatgctga gtgaagtcgc caaacgctta ggaccagacg tttatcaaac
     4381 ctttactgaa ggtcgcagtc agcatgaatg gatcaaatat ctccatgcga aaacgaagga
     4441 acgtaaccct gagatgctcg actacgagga gatgaaaacg accgggatct ttaagaaaaa
     4501 atgcccggaa gaacactacg tcgctttccg cgcattccgt gaagatccac aggcaaaccc
     4561 gttgaaaaca ccttcgggga aaatcgaaat ttattctgaa cgactggcga agattgcaga
     4621 tacctgggaa ttgaaaaaag atgaaattat tcatcccctt cctgcgtata ccccaggttt
     4681 tgatggctgg gacgatcccc tgcggaaaac ctatccactg cagttaacgg gcttccacta
     4741 caaagcgcgt acccactcca gctacggcaa tattgatgtg ttacagcagg cctgcccaca
     4801 agaggtgtgg atcaacccca ttgatgctca ggcacgcggt atccgtcatg gcgataccgt
     4861 gcgggtattt aacaataatg gagaaatgct gattgccgca aaagtgactc cgcgtattct
     4921 gcctggcgtt accgccatcg ggcaaggtgc gtggcttaaa gcggatatgt ttggtgaccg
     4981 ggtcgatcac ggcggcagta tcaatattct gacctctcac cgcccttcac cgctggcaaa
     5041 gggaaacccg tcgcacagca atcttgtcca gatcgaaaag gtttaaggag tagcccatga
     5101 ccacacaata tggatttttt atcgattcca gccgctgtac tggctgtaaa acctgcgaac
     5161 tggggtgcaa agattttaaa gatcttggcc cggaagtcag tttccgccgt atttatgaat
     5221 atgctggcgg cgactggcag gaagataatg gcatctggca ccagaacgtg tacaaaagtc
     5281 tgccccagcg gcgcaatgca taagcgtgaa gatggctttg tggtggttga tgaagatgtt
     5341 tgtatcggct gccgctactg ccacatggct tgtccgtacg gcgcaccaca gtacaatgct
     5401 gaaaaagggc acatgacgaa gtgcgatggt tgttattcgc gcgtcgccga ggggaaacaa
     5461 cccatatgca tcgaatcctg cccgctgcgt gcacttgagt tcggtccaat tgaagagctg
     5521 cgccagaagc acggcactct ggctgccgtc gcgccactgc cgcgcgcgca tttcacaaaa
     5581 cccaatatcg ttatcaaacc taacgccaac agccgcccga cc
//
LOCUS       DAZPEB010000213         5595 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZPEB010000213 DAZPEB010000000
VERSION     DAZPEB010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5595
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.218"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..401)
                     /locus_tag="ACHURJ_003674"
     CDS             complement(<1..401)
                     /locus_tag="ACHURJ_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal domain-containing
                     protein"
                     /protein_id="HFR1282454.1"
                     /translation="MGILEQEMKRLAQQAGGSHKTVHDRIKLVQRFCERLVLAQNVQI
                     RRVEQLKARYIEGYVRERLAQGITKRSLQNEMAAVRCILKQAGRDRLAQSERLNNRSL
                     GLSGASRNGTKRAITPEHYRHVLETARAKDPG"
     gene            960..1106
                     /locus_tag="ACHURJ_003675"
     CDS             960..1106
                     /locus_tag="ACHURJ_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282455.1"
                     /translation="MYRIKTLASVAMFCDPVFAEVCTGKRIDRPDNRRFFQVVKRWAL
                     STTA"
     gene            complement(1266..1583)
                     /locus_tag="ACHURJ_003676"
     CDS             complement(1266..1583)
                     /locus_tag="ACHURJ_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016161430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFR1282456.1"
                     /translation="MDRGEIWLVSLDPTTGHEQSGKRPVLIVSKASFNTFTRLPVVVP
                     VTSGGNFAREAGFTVSLDGAGTKTTGVIRCDQPRTIDMGARSGKRLERIPDAVVNEVL
                     ARL"
     gene            complement(1584..1841)
                     /locus_tag="ACHURJ_003677"
     CDS             complement(1584..1841)
                     /locus_tag="ACHURJ_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFR1282457.1"
                     /translation="MYTTRLKKVGGSVMLAVPPAVLKTLGLSTDSEVGMTIDNGCLII
                     EPQKRPRYSLEELLAQCDPHAEISEEGREWIDAPAVGKEIL"
     gene            complement(2190..2396)
                     /locus_tag="ACHURJ_003678"
     CDS             complement(2190..2396)
                     /locus_tag="ACHURJ_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1282458.1"
                     /translation="MMLQDWHPADIIAGLKKRGTSLSALSRQAGLASSTLANALTRRW
                     PKGERLIAEALGIKPEQIWPSRYR"
     gene            complement(2393..2854)
                     /locus_tag="ACHURJ_003679"
     CDS             complement(2393..2854)
                     /locus_tag="ACHURJ_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282459.1"
                     /translation="MATIPTQTHPLLAVPFSAATDFTVLADHCENVAETLIESEDPVL
                     RLALCGRLAACLTLLQPTLNDPIPSHLTDSLTVDALPADMPRFVPDAELLCDYSLTLA
                     QLLIGQGMQPKVEQTITGLLCELVWYFAAEMKAPRWLRTADGVKFIDEVAA"
     gene            complement(3225..4829)
                     /locus_tag="ACHURJ_003680"
     CDS             complement(3225..4829)
                     /locus_tag="ACHURJ_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LA2681 family HEPN domain-containing protein"
                     /protein_id="HFR1282460.1"
                     /translation="MITKELVDESNFISQLVDIGRFDESYQQSVAFLNKLERITIRND
                     NYFIVLANIAGALVDIGQMQNNKNASELGCNLMEDNKEAFISVLGECLYYYNYGNALS
                     NLVSITNPNDHTFKSIEELVSLKNIYWRSFKLSAEEQEEFQAELSVNLANSLRSQFRL
                     SESLRYYDLTNLKGLDIPQAWVNRSMSLMVLNLISSSFSIKLLKEVRAGYIKASVSKN
                     IPPQWESFYLERIAQTNEKIAEYAVDDETDEHDEALTQQEFEVLSPYRQFCLRNHLTL
                     SEHGLYCPCVGSATDDLVIASRGGVTGDFIIPMEMVLNRLKSEFSLARHLYFDYLHPQ
                     NTDYIKYESHFLELYNDEVLGIEIEKIRTAFRLCFGILDKIAVAICELYNLYPTTKKG
                     TPQKNIYFQNFWQLDVDNRRQLFEKVKSPGLLALYSIATDLNKDKGGELAFYKEWRNG
                     LEHKFLVVHKSDKPEDIYESYQLIEDILFIKESDFIHHFEHLLQITRSAIFSFAFMVR
                     QEGMKEKKEGIHYMTRELHAINYSAD"
     gene            complement(4842..5207)
                     /locus_tag="ACHURJ_003681"
     CDS             complement(4842..5207)
                     /locus_tag="ACHURJ_003681"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282461.1"
                     /translation="MVSDKRADIIVSLPQIKIPIEIKRDYHRDVWTALNGQLDKLYTK
                     NPDAAGHGIYLVFWFGSARPNSLPHLAKNTSQPENASAMENMLNETVPVDKRDRLSAI
                     VIDVSCEGIPPVEAPKSSV"
     gene            complement(5235..>5595)
                     /locus_tag="ACHURJ_003682"
     CDS             complement(5235..>5595)
                     /locus_tag="ACHURJ_003682"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282462.1"
                     /translation="DASEVLERLLLLPECESYRDHLSHAQSNQKIRYRESQFHHADWK
                     QTVSTLMNKTPANIMDLYSLLLDHIRDISNRIANENTDIYKQFWNEDSYGRTLTPKPE
                     ESCRNIFLDLLRVRLNP"
BASE COUNT         1447 a         1346 c         1306 g         1496 t
ORIGIN      
        1 cccggatctt ttgcgcgggc ggtttccagc acatggcggt aatgctccgg cgtgatggcc
       61 cgcttcgtac cgttacggga tgcgccagac aggcccagcg agcggttatt cagtcgctca
      121 ctctgcgcca gcctgtcacg cccggcctgt ttcagaatgc agcgcaccgc cgccatctca
      181 ttttgcaggg aacgcttcgt gataccctgc gccagtcgtt cacgaacata tccctcaata
      241 taccgcgcct taagctgctc cacccggcgg atctgcacat tctgtgccag aaccagacgc
      301 tcacaaaacc gctgcaccag cttaatacgg tcgtgaacgg tcttatggct gccgcccgcc
      361 tgttgtgcca atcgcttcat ttcctgctcc agaatcccca tacattcccc ctttcatgat
      421 tgccgcaaac tcatccccaa aaactcaaca ctgatacagc gttccccggc gcacaggccg
      481 atagcccctg agttttgtcg gggctgcgaa cgatacctga gcgccggggc ggcagccgtt
      541 gaggtgtggt ggggcttcgg ttgtgctctc agagcagccg cccgcttgcg cgggtcatcc
      601 cccggatcgt ccagatccgc ctcggtatcg gttcatattc tccttatggg taaatggcct
      661 aaaaaggcgg tgtcagaatc cagccatgag ctggcgcagt tgagttttct ggtgcaggtc
      721 gaaagggctt gcgtgcgtca ctttcccgct ttgcgcggtt aaagtgacgc acggatctgg
      781 tgcagcatct gagatttatc gtctggtgca gttttacggt cacgcggcag caaagcctga
      841 cggcaatgca ggccgcagcc cgtaaggtaa aaggcgctga gggtgtcagc agatgcaggt
      901 tagccgggag aattccggcg cggtgcagat tcagaccagc aggctggcga agtaaaagga
      961 tgtatcgtat taaaactctg gcctccgtcg cgatgttctg cgacccggtg tttgcagagg
     1021 tctgcaccgg taaacggatc gaccgtcctg ataacaggcg cttctttcag gttgtgaagc
     1081 gttgggcgct gtctacgaca gcatagagca agggggagac tacgcctgtg ccgctggcgc
     1141 ggaaaggaga aactgttttt acctgcaaaa ggatttttta atcacttgtt gttataaaaa
     1201 atgagggcgc tgtatgtgac agcgccctcg ttcaggttac ataaaaaata tcagctcaga
     1261 atagcttaca gtcgagccag cacctcattc acgacagcat cgggtatacg ttccagccgc
     1321 ttaccgctcc gagctcccat atcaatggtt ctgggctggt cgcagcgaat aacgcccgtt
     1381 gtttttgttc ccgccccgtc cagtgaaacg gtaaaaccgg cttcgcgggc aaagtttccg
     1441 ccgctggtga cagggacaac aacaggcaac cgggtgaacg tgttaaacga tgccttcgat
     1501 acgatgagta ccggacgttt tccactttgt tcatggccag tggtgggatc cagtgaaacg
     1561 agccagattt ctcctctgtc catctacagg atctccttgc ccaccgcagg cgcatcaatc
     1621 cattcccgac cctcttcgct tatttcagcg tgcggatcgc actgcgccaa cagttcttcg
     1681 agcgaatagc ggggacgttt ttgaggttca ataatcaggc agccattatc aatggtcatg
     1741 cccacttcgc tgtccgtcga cagccctagc gttttcagca ctgcgggggg aaccgccagc
     1801 atgacggatc cgccaacctt tttcaggcga gtggtataca tagagcacct ccggatatta
     1861 tattttaata taacatccat gatagacgat gcacaactat tcatcagatt cattgtggtt
     1921 tccttagtgt aataaagcgt gaagaaagcc ttaatccatc ggaagacaga tccggcagtt
     1981 ttcaaaggcg acattgccgg taaaagggca accgtcctga tacatcaggc gcttctttca
     2041 ggtcatgaag catttcgtac aggcgggagc ttagtgcgta acaaagaagg ccgataatag
     2101 gaaactgctt ttgcagttca aaggattatg acggttcgga taaacaaaat gcggcgcagc
     2161 ttccgtatcc gggctgcgcc gcaggaacat cagcggtagc gcgaaggcca gatttgctca
     2221 ggcttgatgc ctaacgcttc ggcaattaac ctttcgcctt tgggccagcg gcgggtaagc
     2281 gcattagcca gcgtggagga cgccagacct gcctgacgcg aaagcgctga cagcgaagtg
     2341 cctcgttttt tcagcccggc gataatgtcg gcaggatgcc agtcctgcag catcatgcag
     2401 ccacctcgtc gataaacttc acgccatcgg cggtgcggag ccagcgcggg gctttcattt
     2461 cggcggcaaa ataccagacc agttcacata ataagcctgt tatcgtctgc tccacttttg
     2521 gctgcatccc ctgcccgata agaagttgcg ccagcgtcag tgagtaatca caaagtaatt
     2581 ctgcgtcggg gacaaatcgg ggcatatcgg caggtagagc atcaacggtc aggctgtcag
     2641 tcagatgaga cgggatgggg tcgttcagcg tgggttgcag caaagtgaga caggcggcaa
     2701 gtcgcccgca aagcgccagc cgcagcaccg gatcttcgct ttcgatcagg gtttcggcga
     2761 cgttttcgca gtggtctgcc agtacggtaa aatctgtggc agcactgaag ggaacagcaa
     2821 gtaacgggtg tgtctgagtg gggatcgtag ccataaaggc agcctccagt atgtggtttg
     2881 aaaaccacca ccagaggttc caatctcatg ggtggtggac tgaacgaggt tggaactacc
     2941 ggccatactg gaaccggcgc accttacggt gcccccgccc agcccaccat aattttggtg
     3001 tagctaagcg cgcgacataa aaaaagacgc agacgcgtca cgtgtcgcca gtatggttat
     3061 atttcggggt tccaatcccg gcacctgatt ttgcaggtgc gggtaaaaga taccgctttc
     3121 ggttgaaaac ggcaagggga agtcgtggaa tagagatcgc agattgttat aaatatacac
     3181 aatagttaat gttaacaaag acggcacata tattaacttt actctcaatc tgctgaatag
     3241 tttatcgcat gtaattctcg ggtcatatag tggataccct ccttcttttc tttcatcccc
     3301 tcttgcctca ccataaaagc aaacgaaaaa attgctgaac gagttatctg aagtaaatgc
     3361 tcaaaatggt gaataaaatc actttctttg ataaaaagaa tatcttctat tagttgataa
     3421 gattcgtaaa tgtcctcagg tttatcactt ttatgtacaa ctaaaaactt atgctctaaa
     3481 ccattgcgcc attctttata gaaagccaac tccccaccct tatctttatt caggtcggta
     3541 gcaatactgt acagcgccag aagccctggt gattttacct tttcaaataa ttgtcttctg
     3601 ttatcaacat ctaactgcca gaaattctga aaatagatat tcttctgagg tgtacctttt
     3661 tttgttgttg ggtaaagatt ataaagttca catatggcta ccgcaatttt atcaagaata
     3721 ccaaagcaaa gacgaaatgc cgtcctgatt ttttcaattt ctattcctaa aacttcgtca
     3781 ttatacaatt ccagaaaatg gctttcgtac ttaatgtaat ccgtattttg tggatgaaga
     3841 taatcaaaat aaagatggcg tgcgagtgaa aactccgatt taaggcgatt taataccatc
     3901 tccatgggaa tgataaaatc acctgttaca ccgcctcgcg aagcaatcac aagatcatct
     3961 gtagcactcc ctacacatgg gcaatacagg ccatgttctg ataaagtcag atgatttcta
     4021 agacaaaact gtcgatatgg gcttaacact tcgaactctt gttgtgtaag tgcttcatcg
     4081 tgttcgtcag tttcatcatc aacagcatat tctgcgattt tctcgttagt ctgcgctatg
     4141 cgttctaaat agaacgattc ccattgtggt gggatatttt ttgatacaga cgctttaata
     4201 tatcctgctc tcacttcttt tagtaacttg attgaaaatg aagatgaaat aagattgagg
     4261 accataagtg acatcgaacg gttgacccaa gcctgtggta tatctaatcc cttaagattg
     4321 gtaagatcat aatatctcaa tgactcactg agccgaaatt gacttcttaa tgaattagcc
     4381 aaattaacag atagttcagc ctgaaattcc tcctgctcct cagcagatag cttaaaagaa
     4441 cgccagtaga tattttttaa ggaaaccaat tcttctatcg atttgaatgt atggtcattg
     4501 gggttagtaa tgctcaccag attagatagc gcattaccat agttataata gtaaaggcac
     4561 tctcctaaca ctgatataaa tgcttctttg ttatcttcca tcaggttgca accaagctct
     4621 gacgcatttt tattattttg catctgcccg atgtcaacaa gagcacccgc aatgttcgcc
     4681 aatactataa agtagttatc gttcctgatt gttattcttt ccagtttgtt caggaacgca
     4741 actgactgtt gataagattc gtcaaatctt ccaatatcaa ccagttggct aatgaaatta
     4801 ctttcatcga caagttcttt tgttatcaaa ataaattttc cttatacaga ggatttcggt
     4861 gcttcaacag gaggtatgcc ctcacaggag acatctataa ctatcgctga taagcggtct
     4921 cttttatcaa ccggcacagt ttcattaagc atattttcca ttgcactggc gttttcaggt
     4981 tgagatgtat ttttcgccaa atgaggcagg gaattcggtc gagcagaacc aaaccaaaaa
     5041 accagataga taccgtgacc agcagcatcg gggtttttag tataaagctt gtccaattgc
     5101 ccattaagtg cagtccagac atcacgatga taatcacgct ttatctcgat aggtattttt
     5161 atctgcggta gtgaaacaat gatatctgcc cttttatcag aaaccatgtg tccttctggt
     5221 tcacagctta ttttttatgg attaagccgg acacggagta aatccagaaa aatattacgg
     5281 caactctctt caggtttagg cgttagggta cggccatagc tgtcttcatt ccagaattgc
     5341 ttataaatat cggtattttc attggcaatt cggttagaaa tatcccgtat atgatcaagc
     5401 aataatgagt aaagatccat tatgttggca ggcgttttat tcatcaatgt acttacagtc
     5461 tgcttccagt cggcatgatg gaactgtgat tctctataac gaattttctg attgctttgg
     5521 gcatgtgaga gatgatcccg ataactttca cattcaggca gcagtaataa gcgctcaagt
     5581 acttcactag catcg
//
LOCUS       DAZPEB010000214         5566 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZPEB010000214 DAZPEB010000000
VERSION     DAZPEB010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5566
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.219"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(35..772)
                     /gene="bamD"
                     /locus_tag="ACHURJ_003683"
     CDS             complement(35..772)
                     /gene="bamD"
                     /locus_tag="ACHURJ_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFR1282463.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENSYRQMQMNAQAEKVAKIIAANSSNT"
     gene            907..1887
                     /gene="rluD"
                     /locus_tag="ACHURJ_003684"
     CDS             907..1887
                     /gene="rluD"
                     /locus_tag="ACHURJ_003684"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFR1282464.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNLDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPEDMVELIEVMRADFEEHKDEVDWL"
     gene            1884..2615
                     /gene="yfiH"
                     /locus_tag="ACHURJ_003685"
     CDS             1884..2615
                     /gene="yfiH"
                     /locus_tag="ACHURJ_003685"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFR1282465.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     EHVEENRKQLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2745..5318
                     /gene="clpB"
                     /locus_tag="ACHURJ_003686"
     CDS             2745..5318
                     /gene="clpB"
                     /locus_tag="ACHURJ_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFR1282466.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1385 a         1327 c         1510 g         1344 t
ORIGIN      
        1 ctcaagggct gccgttttgt gtttcaggtt tctgttatgt attgctgctg tttgcggcga
       61 tgattttcgc tactttttca gcttgcgcat tcatctgcat ctgacggtat gaattttcca
      121 tcagtggcag cgcatcacgc gtagcctggg tatccgggta gtcgcgcaac atgccttcta
      181 cgcggttaac gacggcaacc catgcaccac gttctgtata gtactcggca acggagtatt
      241 catatttcgc cagacgatct ttcaggaata ccagacgttt ggttgcatcg gtagtgtact
      301 gactgttcgg atagccgcgc accagtttgg aaaagtcact aaacgcagct cgtgcatgtt
      361 gaggatcgcg atcgctacgg tcaacgccaa agaacccttg cagcgcactg tcatccagcg
      421 ccatattggt caggccacgc atgtacatga cataatcgat attcggatgg gtcgggttaa
      481 ggcgaataaa acgatcgatg gcagcctgtg ctaacggcaa atcggcgttt ttatagtagg
      541 cgtagatgag atccagctgc acctgctgcg aatacggacc aaacggatag cgattatcta
      601 atgcttccag ttgcgttatt gcctgtctcc agttaccgtc ctgcagcttt tgttgtgcag
      661 tcgcgtaaat ttcatttggt ggattatcag gtacttcttc ctttgacccc gagcaacccg
      721 ccaaaaacag gcttagtgtg gcggctgcca ccagatattt catgcgcgtc atgacgtttt
      781 gactttcctc aaaatgtaat acgggagatt ctctgttcct gctcccggtt aagaccagct
      841 acaatagcac actatattaa acggcaaagc cgtaaaaccc caacgataaa cgaagaagca
      901 gtatatatgg cacaacgagt acagctcact gcaacggtgt ccgaaaacca actcggtcaa
      961 cgcttagatc aggctttggc cgaaatgttc ccggattatt cacgttcgcg aataaaagaa
     1021 tggatcctcg accagcgcgt gctggtaaac ggcaaagttt gtgataagcc gaaagaaaaa
     1081 gtattgggtg gcgagcaggt tgccatcaac gctgagattg aagaagaagc gcgttttgaa
     1141 ccgcaggata tcccgctgga tatcgtctat gaagatgaag atatcatcgt cattaataaa
     1201 ccgcgcgacc tggtggtaca tcctggcgcg ggtaacctgg atggcacggt actgaatgcg
     1261 ttgcttcatt attatccgcc cattgccgat gtaccgcgtg cgggcatcgt ccatcgtctg
     1321 gataaagaca ccactggcct gatggttgtg gcaaaaaccg ttccggctca gacgcgttta
     1381 gtcgaatctt tgcaaaggcg tgaaattact cgtgagtatg aagcggtggc aattggtcat
     1441 atgaccgctg gcggtacagt agatgaacca atcagccgcc acccgaccaa acgtactcac
     1501 atggcggtgc atcccatggg caaaccggcg gtcactcact atcgcatcat ggaacacttc
     1561 cgtgtgcaca cgcgtctgcg gttgcgtctg gaaaccggac gtacgcacca gatccgcgtg
     1621 catatggcgc atattaccca tccgctggtg ggcgatccgg tttatggtgg ccgtccgcgt
     1681 ccgccaaaag gtgcttcgga agcgtttatc tccacgctgc gtaagtttga ccgccaggcg
     1741 cttcatgcaa ctatgctgcg tctttatcac ccgatctccg gcatcgaaat ggaatggcat
     1801 gcgcctattc cagaagatat ggtggagttg attgaggtta tgcgcgccga tttcgaagag
     1861 cataaggatg aagtggactg gttatgagta agctgattgt cccgcagtgg ccgctgccaa
     1921 aaggtgttgc ggcctgtagc tctacccgta tcggcggcgt gagcttgcca ccttacgact
     1981 ctctcaacct tggcgctcat tgtggtgata acccggagca cgtagaggag aatcgcaagc
     2041 aactttttgc tgcgggaaat ttgccttcaa aaccggtctg gcttgagcag gtacacggca
     2101 aagatgtgct taagctcact ggcgaacctt atgcctcaaa acgggcggat gcctcttata
     2161 gcaatacgcc cggcacggtt tgcgcagtga tgactgccga ctgcctccct gtgctgtttt
     2221 gcaatcgagc gggaacggaa gtcgccgccg ttcatgctgg ctggcgtgga ctgtgcgcag
     2281 gcgtgctgga agagacggtt tcctgttttg ctgataaacc tgaaaacatt ctcgcctggt
     2341 tagggccggc aattggtcca cgcgcgttcg aagtgggggc ggaggttcgc gaggcgttta
     2401 tggcagtaga cgctaaagca agtgcagctt tcattcagca tggtgataag tatctggcgg
     2461 atatttatca gcttgcccgg cagcgtctgg cgaacgtggg tgttgagcaa attttcggcg
     2521 gcgaccgttg tacatatacg gaaaatgaga ctttcttctc ttatcgtcgc gacaagacca
     2581 ccggtcgtat ggcaagtttc atttggctga tataacctaa agaatcaaga cgattcggta
     2641 cgcgtgattt tcttttcaca ttaatctggt caataacctt gaataattga gggatgacct
     2701 catttaatct ccagtagcaa ctttgatccg ttatgggagg agttatgcgt ctggatcgtc
     2761 ttactaataa attccagctt gctcttgccg atgcccaatc acttgcactc gggcacgaca
     2821 accaattcat cgaaccactt catttaatga gcgccctgct gaatcaggaa gggggttcgg
     2881 ttagtccttt attaacatcc gctggcataa atgctggcca gttgcgcaca gatatcaatc
     2941 aggcattaaa tcgtttaccg caggttgaag gtactggtgg tgatgtccag ccatcacagg
     3001 atctggtgcg cgttcttaat ctttgcgaca agctggcgca aaaacgaggt gataacttta
     3061 tctcgtcaga actgttcgtt ctggcggcac ttgagtctcg cggcacgctg gccgacatcc
     3121 tgaaagcagc aggggcgacc accgccaaca ttactcaagc gattgaacaa atgcgtggag
     3181 gtgaaagcgt gaacgatcaa ggtgctgaag accaacgtca ggctttgaaa aaatatacca
     3241 tcgaccttac cgaacgagcc gaacagggca aactcgatcc ggtgattggt cgtgatgaag
     3301 aaattcgccg taccattcag gtgctgcaac gtcgtactaa aaataacccg gtactgattg
     3361 gtgaacccgg cgtcggtaaa actgccatcg ttgaaggtct ggcgcagcgt attatcaacg
     3421 gcgaagtgcc ggaaggattg aaaggccgcc gcgtactggc gctggatatg ggcgcgctgg
     3481 tggctggggc gaaatatcgc ggtgagtttg aagaacgttt aaaaggcgtg cttaacgatc
     3541 ttgccaaaca ggaaggcaac gtcatcctgt ttatcgacga attacatacc atggtcggcg
     3601 cgggtaaagc cgatggcgca atggacgccg gaaacatgct gaaaccggcg ctggcgcgtg
     3661 gtgaattgca ctgcgtaggt gccacgacgc ttgacgaata tcgccagtac atcgaaaaag
     3721 atgctgcgct ggaacgtcgt ttccagaaag tgtttgttgc cgagccttct gttgaagata
     3781 ccattgcgat tctgcgtggc ctgaaagaac gttacgaatt gcaccaccat gtgcaaatta
     3841 ctgacccggc aattgttgca gcagcgacgt tgtctcatcg ctacattgct gaccgtcagc
     3901 tgccggataa agccatcgac ctgatcgatg aagcagcatc cagcattcgt atgcagattg
     3961 actcaaaacc agaagaactc gaccgactcg atcgtcgtat catccagctc aaactggaac
     4021 aacaggcgtt aatgaaagag tctgatgaag ccagtaaaaa acgcctggat atgctcaacg
     4081 aagaactgag cgacaaagaa cgtcagtact ccgagttaga agaagagtgg aaagcagaaa
     4141 aggcatcgct ttctggtacg cagaccatta aagcggaact ggaacaggcg aaaatcgcta
     4201 ttgaacaggc tcgccgtgtg ggggacctgg cgcggatgtc tgaactgcaa tacggcaaaa
     4261 tcccggaact ggaaaagcaa ctggaagccg caacgcagct cgaaggcaaa actatgcgtc
     4321 tgttgcgtaa taaagtgacc gacgccgaaa ttgctgaagt gctggcgcgt tggacgggga
     4381 ttccggtttc tcgcatgatg gaaagcgagc gcgaaaaact gctgcgtatg gagcaagaac
     4441 tgcaccatcg cgtcattggt cagaacgaag cggttgatgc ggtatctaac gctattcgcc
     4501 gtagccgtgc ggggttggcg gatccaaatc gcccgattgg ttcattcctg ttcctcggcc
     4561 caacgggtgt ggggaaaact gagctttgta aggcgctggc gaactttatg tttgatagcg
     4621 acgaggcgat ggtccgtatc gatatgtccg agtttatgga gaaacactcg gtgtctcgtc
     4681 tggttggtgc gcctccggga tatgtcggtt atgaagaagg tggctacctg accgaagctg
     4741 tgcgtcgtcg tccgtattcc gtcatcctgc tggatgaagt ggaaaaagcg catccggatg
     4801 tcttcaacat tctgttgcag gtactggatg atggtcgtct gactgacggg caggggagaa
     4861 cggtcgactt ccgtaatacg gttgtcatta tgacctctaa cctcggttcc gatttgattc
     4921 aggaacgctt cggtgaactg gattatgcgc acatgaaaga gctggtgctc ggtgtggtaa
     4981 gccataactt ccgtccggaa ttcattaacc gtattgatga agtggtggtc ttccatccgc
     5041 tgggtgaaca gcacattgcc tcgattgcgc agattcagtt gaaacgtctg tacaaacgtc
     5101 tggaagaacg tggttatgaa atccacattt ctgacgaggc gctgaaactg ctgagcgaga
     5161 acggttacga tccagtctat ggtgcacgtc ctttgaaacg tgctatccag cagcagatcg
     5221 aaaacccgct ggcacagcaa atactgtctg gtgaattggt tccgggtaaa gtgattcgcc
     5281 tggaagttaa tgaagaccgg attgtcgctg tccagtaatg ataaaacgag cccttcgggg
     5341 ctcgtttttg tctataagtt agacggaaaa gactatattt aagatgtttt gcctgaaaaa
     5401 tgagcgaacg ataaagtttt tatatttttc gcttgtcagg ccggaataac tccctataat
     5461 gcgccaccac tgacacggaa caacggcaaa cacgccgccg ggtcagcggg gttctcctga
     5521 gaatctcaac agagaaaagc aaaaaaatgc ttgactctgt agcggg
//
LOCUS       DAZPEB010000215         5533 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZPEB010000215 DAZPEB010000000
VERSION     DAZPEB010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5533
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.220"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(152..586)
                     /locus_tag="ACHURJ_003687"
                     /pseudo
     CDS             complement(152..586)
                     /locus_tag="ACHURJ_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Catalyzes the hydrolysis of AMP to form adenine and
                     ribose 5-phosphate using water as the nucleophile;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
     gene            complement(585..956)
                     /locus_tag="ACHURJ_003688"
                     /pseudo
     CDS             complement(585..956)
                     /locus_tag="ACHURJ_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            1283..1358
                     /locus_tag="ACHURJ_003689"
     tRNA            1283..1358
                     /locus_tag="ACHURJ_003689"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1316..1318,aa:Asn,seq:gtt)
     gene            complement(1414..2349)
                     /gene="ldtA"
                     /locus_tag="ACHURJ_003690"
     CDS             complement(1414..2349)
                     /gene="ldtA"
                     /locus_tag="ACHURJ_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR1282467.1"
                     /translation="MMRRVNIFCSFALLFASHTSLAVTYPLPPEGSRLVGQSYTVTVP
                     DHNTQPLETFAAQNGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYEFDRKVPLPVTPSLR
                     AFISGQEVDVNRANAALQRRSGMPVPISSGSRQMF"
     gene            complement(2411..3490)
                     /gene="cobT"
                     /locus_tag="ACHURJ_003691"
     CDS             complement(2411..3490)
                     /gene="cobT"
                     /locus_tag="ACHURJ_003691"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFR1282468.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGVPHVGKKAVLVMCADHGVWEEGGAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVYVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRHQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRAPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(3502..4245)
                     /gene="cobS"
                     /locus_tag="ACHURJ_003692"
     CDS             complement(3502..4245)
                     /gene="cobS"
                     /locus_tag="ACHURJ_003692"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFR1282469.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILALLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(4242..4786)
                     /gene="cobU"
                     /locus_tag="ACHURJ_003693"
                     /pseudo
     CDS             complement(4242..4786)
                     /gene="cobU"
                     /locus_tag="ACHURJ_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
     gene            complement(4920..5357)
                     /locus_tag="ACHURJ_003694"
     CDS             complement(4920..5357)
                     /locus_tag="ACHURJ_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HFR1282470.1"
                     /translation="MKTIKPESIALFCLLSGDVDLAKHLADILPITCFVGEDWQQDFQ
                     PLDGGPVQSLSDSYSDYSVLLLIAAESEISVPEWLAHSEGTTVILIAEHNARLLTGPA
                     GGAVALIHYLNEICHSRETAVNDAFCRTLTRGSLAIPQCHLLS"
BASE COUNT         1470 a         1510 c         1227 g         1326 t
ORIGIN      
        1 atacttgctt ttaatatgtg gaaatcatta agacgataat aaaacacgta tccgcccaag
       61 gaagcaaaca gcccacgatt attgcaggct atttgccagg tgtcttactt actccttcac
      121 atctatttct gataagaagg ttcagaactt attatcggaa cggcggctca ttaaaggttc
      181 gtaatttacg cgaatgcagt cggtcgcctt ctgcgcgcag caaatcgatc gcccgaatgc
      241 caatttgcag atgttcggaa atagcgcctt cataaaaacg gttagcctgg ccgggaagtt
      301 taatctcgcc atgcaacggt ttatctgaaa cacacagtag tgtcccgtat ggcacgcgga
      361 aacgatatcc ttgcgcggca atggttgcac tttccatatc aattgctacg gcccggctta
      421 agttaaaacg aagtgcagaa gctgagtaac gtaattccca gttcctgtca tctgtggtta
      481 ccacagtacc agtacgtagc cgctgtttga cttcctcacc gggcctgccg ctcaccagct
      541 tggtggcgtc ataaagcgca cgttgcactt cagcaatgct cgggatagat aacgatctcc
      601 ggaaattcag cgcgaaccgc ctgtaataag ggcatggtga tggatgacgc agcggttcct
      661 ggtgcaaagc caatcgagac ttgccccgat aatgcctgac caacgttatg caccgccagt
      721 tgggcctgtt cacactgacg taaaatggcc cgcgcatggg tatagagaat ttttccggcg
      781 tctgttggcg taacgccccg ctttgtacgg atcaaaagtt gttgatttaa ctcaccttcc
      841 agtgtggcaa cctgctggct gagcgctggt tgtgcgatat gcaatacttc agcagcctgg
      901 gtcaggctac caatatctac aatttttacg aagtatttca ggcgtctgaa gttcatgttg
      961 cctccggttt ttaagaatcg gcccaagtgc cgccattact tacaaccaga ttgcaagatg
     1021 cttgccagtt ttattttggc gttgatgtac aagctaacca actgtcaaat aagagattat
     1081 gatagattcg tcatttgctc ctttaatcag ctgtcgcgtt cccctgccct ataaaaggag
     1141 ggtatgcacc acgatggttc attacccaat aagattgaaa gctcaccact ttgttgaaat
     1201 tgacagcaaa caaacaaaaa aatgcatttc accctttgac atcaccatgc actgccatta
     1261 atatgcgccc cgttcacacg attcctctgt agttcagtcg gtagaacggc ggactgttaa
     1321 tccgtatgtc actggttcga gtccagtcag aggagccaaa ttcaaaaaag cctgctttct
     1381 agcaggcttt ttgctttcta attaccaacg ctcttaaaac atctgtcttg aaccagaact
     1441 aattggcaca ggcattcccg atcgacgttg caacgcagca tttgcgcgat ttacatcaac
     1501 ttcttgcccg ctgataaacg cccgcaaaga tggggttacc ggcaatggca cttttcggtc
     1561 aaactcatat tctgcacgat tgcgcgacaa cggctcatga acttccagcc agttcgagcc
     1621 atctggttca gtggtgtatt ttactggctg gtcgataatt tgcacacgcg tcccaacagg
     1681 aacattatca aacagatatt tgatatcgtc attgcgcaga cgaatacagc cctgacttac
     1741 ccggagccca ataccaaaat tggcattggt accatggatg gcatacaacc tgccaatata
     1801 aatcgcgtac agccccatgg gattatcggg gcccgcagga acaaatgcgg gcaaactctc
     1861 ccctcgtttc gcatattcgc gccgagtgtt cggcgttggc gtccaggttg gagcttcttg
     1921 tttacgttca acggtagtca cccagttacg cggggtttct cgcccagcct ggccgatacc
     1981 aataggaaag acttccacag tattactgtc tggtgggtag taataaagac gcatctcagc
     2041 gacgttaacc acaatccctt tacgaacagt ggcgggcaaa atcagttgct gcggaatggt
     2101 gagttgcgag ccggacttcg gcaagaaaac atcagcaccc gggttcgctt ccagcatgtt
     2161 acttaaccct tgcccgtttt gtgcggcaaa agtctccagc ggctgggtat tgtgatcagg
     2221 aacagttaca gtatacgact gccccactaa acggctaccc tctggaggta atgggtaagt
     2281 taccgccagg ctagtatggc tggcaaaaag cagagcaaat gagcaaaaaa tatttacacg
     2341 acgcatcatg tccctttcct aagtcgcgaa agctatccgt taagtatagc ttttatcaga
     2401 cttttcgttt ttaactgttc aaatcagaag tcgtattccc cggtagaaca atattactgg
     2461 cagcaagttc gcccatgttg ttatatatcg cacaggcagc ttcgatgatg ggcatcgcca
     2521 gagctgcgcc actcccctca cctaaacgca tctccatatt gagataaggc tccagcccca
     2581 aatgcgacag cgctatacgc gctccttttt ctgccgacag gtgagaagga atgagatacg
     2641 gtttgattgc aggagacatc tgacaggctg cgagcgcagc agcataagaa agaaatccat
     2701 ccagcaacac gggtaaaccg caggacgcag cgcctaacat cacgccagcc attccgacca
     2761 aatcaaatcc acccactttt gccaggacat caacgccatc ctgcggattt ggttgattca
     2821 acgtaatcgc ccggcgcaca acatcaattt tattagccag tttatctgtc ggcaggtttg
     2881 cgccaatccc aaccacttct tcaggagccc ggccagtgat tgtgctgact attgccgctg
     2941 ccggtgtcgt gtttgccatc cccagttcac ctacaccaaa cagcgtaaca ccgttttttg
     3001 ccagctcccg cgtataacat atgacgtcca aaagcaactt ttctgcctga tggcgactca
     3061 ttgccggagc tgaagcaata ttgccactac ctcgagcaac acgcatgttg ataagcccgg
     3121 ggataggctc agcagtatca ataccaacat caattacgta gacgttagcg cccgcttgtg
     3181 ctgccagcac acacacgccg gttgttccgc gggtcatatt ttcagcctga atggctgtca
     3241 cttcttttgg ggaaatagcg cccccttcct cccagacgcc gtgatcggca cacatgacca
     3301 gtaccgcttt tttgcccaca tgcggaacgc cattcaaccc cggcattcct gccagttgta
     3361 tggcaagcgc ctccagcctt cccaggctac caacaggttt gagtaacccg tcgatatgcc
     3421 gttgtgcacg cgacatagcg gcaggatcga tagcagggat cgtattcagt aaatcggcaa
     3481 gtgtttgaat gtcacgtctc tttatagcag tgccagcagg aagaccagtt caccaagctc
     3541 tatagcagct cccagcgtat cgccggtttg cccgcccagc gtacgtttga gaagctgacc
     3601 gaggatgaaa atcgccacca tcgtcaccac catagcagcc acaccatgca tacccggcaa
     3661 taatacggca gcaaaaatag cggctaagcc gagtgtgacg caggtttgtc gcccatcaat
     3721 tttgccaatg aacacattgc caagtccttc ttcccgggca taacgatgac gatacatcaa
     3781 taatgcggca gtcccgcggc tgaccgcaca tgctgccgcc agcaatgcaa ggatcggttc
     3841 gccacgcaga gccaactcgc ttaataccag aatctttgcc agtacaacaa aaatcaatgc
     3901 cagaccgccg tgggtgccta aacggctatc acgcatgatt tccaacatgc gatcgcggct
     3961 acgtgcagaa aatacgccat cacaggtatc ggcgaggccg tccaggtgga atcccccggt
     4021 catcagcgcc agcacaagta cgctaaacag tgccgccagt ggtacgccac accatgcctg
     4081 cagcaccatg aagaccagcc cgctaatcgc gccaagcaat aatccaatta aaggaaaagt
     4141 aataataccg cgagaataat gctcgaaatc cagtccctgg gaccagcgac gcggtacagg
     4201 cagacgcgta ataaaagaga gcatcgccca aaataattta ctcatttgat ttttactcca
     4261 atacccgaaa ccaccaacca tacttcattt gccgcagcgg ccagctgctg atttacccgc
     4321 ccggcaatat cacgaaaatg tcgtgccaga cgactctccg gcacaatccc cattcccact
     4381 tcgttagtca ctaatacaac ctttgcggga caacgttggc aggcagcaat caacgactga
     4441 atctcagcat taatcgcctg ttccatcgcc tgataatccc attcatcagg gtctttatcg
     4501 ccgccataat caaacaacag attagtcacc atggtggtaa cgcattccag caatacagcc
     4561 tcatgcgggt taatgtctgc atgaattaat tcatcaagat gttgccagcg ctccactgtg
     4621 tgccagtgcg tcgggcggcc ttgccgatga tgttctatcc gtgcagccat ctcatcatca
     4681 aggatttgcg aggtggcgta tacagaacct gtgaagagtc cccaataagc gcctctgcgt
     4741 ggcgactctt cccgctccgt acgccccccg tcaccgaaat catcataccg actcctgatt
     4801 caattgtatg atggtattca ttgaacagaa caatggatcc cccaccgttg ctgaccgcaa
     4861 aacatttacg cgattaatac tgcgcgtaat ataattaaaa atccagcatt ctcaatccat
     4921 tacgaaagaa gatggcattg cggaatagcc agacttcctc gtgttaacgt ccgacaaaat
     4981 gcgtcattga cagcggtttc tctggaatga cagatttcat tcagataatg gattaatgct
     5041 acagccccac cagccggacc agtcagcaaa cgggcattgt gttctgcaat caaaattacc
     5101 gtcgttccct cagaatgagc gagccattct ggtacggata tctcgctttc agctgctatc
     5161 aatagcagca ctgaataatc gctatacgaa tctgataacg attgcacagg cccaccatca
     5221 agaggctgga aatcttgctg ccagtcctca ccaacaaaac aggttattgg caatatatca
     5281 gccagatgtt tcgccagatc cacatctcca gataagaggc aaaaaagagc tatcgattcg
     5341 ggttttatcg ttttcatgcc tctcgccctc cacttaattt cctgtaatgg cgctctggtt
     5401 gataagccat gaaacaatat tgccacacct tgaatgcagc cctggtgaag tggcacactg
     5461 aatttggcca cctgaacaga ggtgatatgc tcacctcaga acaacacagg tgctccaatg
     5521 aaaaaaagaa att
//
LOCUS       DAZPEB010000216         5531 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZPEB010000216 DAZPEB010000000
VERSION     DAZPEB010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5531
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.221"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(195..404)
                     /locus_tag="ACHURJ_003695"
                     /pseudo
     CDS             complement(195..404)
                     /locus_tag="ACHURJ_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006083112.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            643..936
                     /locus_tag="ACHURJ_003696"
     CDS             643..936
                     /locus_tag="ACHURJ_003696"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282471.1"
                     /translation="MIGILKESPKDPDSLKVISITTPARGFSKYGFGSTEHGWFTTIT
                     YNAKNSYGGYVGAKSITFVYLNGDYKVAVPMSNGERVMLDKVSFSCKDNCQTK"
     gene            complement(964..1119)
                     /locus_tag="ACHURJ_003697"
     CDS             complement(964..1119)
                     /locus_tag="ACHURJ_003697"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282472.1"
                     /translation="MNSKQKCSHSNLEKEYYLGSATGDYKCLDCGEVVFGKDWPEKER
                     SKNDNEE"
     gene            2082..2231
                     /locus_tag="ACHURJ_003698"
     CDS             2082..2231
                     /locus_tag="ACHURJ_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282473.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGPPV
                     GREAL"
     gene            3312..3536
                     /locus_tag="ACHURJ_003699"
     CDS             3312..3536
                     /locus_tag="ACHURJ_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="HFR1282474.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYY
                     LSAETAARVRRGDEAVHSSEDVRKSLGLDD"
     gene            3520..3786
                     /locus_tag="ACHURJ_003700"
     CDS             3520..3786
                     /locus_tag="ACHURJ_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFR1282475.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMDLRIAVAADPRQSG
                     KPLKGELGEFWRYRVGDYRILCEIRDDELVILAATIGHRREVYD"
     gene            complement(3833..4102)
                     /locus_tag="ACHURJ_003701"
     CDS             complement(3833..4102)
                     /locus_tag="ACHURJ_003701"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282476.1"
                     /translation="METITHPTLVQLVAAGAVRVVIAVGQPGGWTLLVRYGLAERALA
                     AQRSKQLRVFRKLDTLVLYLQQIGVCRFEVDATGFMGAPRQIRSS"
     gene            complement(4290..4388)
                     /locus_tag="ACHURJ_003702"
     CDS             complement(4290..4388)
                     /locus_tag="ACHURJ_003702"
                     /inference="COORDINATES: protein motif:HMM:NF015750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR1282477.1"
                     /translation="MATIIVHCPRCQSDCVCRHGKGAVSVPCWRVF"
     gene            complement(4430..5449)
                     /locus_tag="ACHURJ_003703"
     CDS             complement(4430..5449)
                     /locus_tag="ACHURJ_003703"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFR1282478.1"
                     /translation="MLYSIQFLRGIAALLVVMAHTAHKGNIYNTETLSWFHIGGSGVD
                     LFFIISGFIMCYTTHNKNLTIISFLTLRVQRIIPLYWVFTTLALTVFVISPSLVNSSG
                     GETSIIDSYFLIPSSAKYLVNNGWTLSYEFYYYLIFSLFLVAFSSTKLRYACIIATLL
                     ILSSFGLIFQPEDIVVDFLLSSWLYEFAIGVILFVAYDRGLVKANFGYISVTLALAFL
                     LYKNFNMEYLSGAPRVISIGVPMGLLFYGFLSFEGYFKRKKHFISNLFERIGDSSYSL
                     YLSHPFVLSPMAIIASKLGVTNSFLFGVILIGSSVFIGYATYVIIEKRIIYFTKIKLV
                     MPSVG"
BASE COUNT         1547 a         1259 c         1216 g         1509 t
ORIGIN      
        1 aaaaaaaatt taagctttcc aataagttag ctagctaaag gccatgattg gcccaataat
       61 agtattctac caataaacca ccagttaata caccgccaac ggcacccgct gttttataaa
      121 cgcttcttgt ttggcgagta atgagttctg aaccagagct tacccagatg cgacatccta
      181 tttgaggggc gcaggatacg gcgaaacagg gcaaaaaacc ctgcagcttc tctatctccg
      241 agccgactgg catcttcccg atacgccaca tccagcaccc aatgttgctg attttcaatg
      301 tgccaatgct ggcgaaccgc tttggctgcc tgttccggcg ctatctccag cgagctgacg
      361 taataatggg tatccaagct tagagcctat cccaataggc tcttaaagtc ggcaacgtct
      421 atttattttc acagcgcata acggatgtta tggtaaatct atctataaat atctattata
      481 gtggatttcc tatagttgtt ttggaaaatg acaaggatat atatgaaaat aattaagtgg
      541 gcttcactgt ctggtgttct gttaatatct gcttgtggtg tttcttagaa cgaagtaaat
      601 agtgctaatt atggtttaaa accaatagat aatgatgcga aagtgattgg tatacttaaa
      661 gagagtccaa aagatcctga ttctctaaaa gttatatcaa taaccacccc cgccagaggt
      721 ttttctaagt atggctttgg ctctacagag catggctggt tcactactat cacatataat
      781 gcaaaaaact cttatggcgg ttatgttggt gctaagtcca ttactttcgt ttacttaaac
      841 ggagattata aagtggcagt gccaatgtct aatggtgaga gggttatgtt agataaagtg
      901 tcattttcat gtaaagacaa ttgccaaact aaatgataaa aaaggtcgca atagcggctt
      961 tttttattct tcgttatcat ttttacttct ttctttttct ggccagtcct tcccaaaaac
     1021 aacctccccg caatccaaac atttataatc tcctgtggcg ctacccaagt agtattcttt
     1081 ttctagattt gaatgagaac atttttgttt gctgttcact acagtcacct ttggatattc
     1141 atttattcgt gaatgatttt tatctatacc gacattgtta aatgatggaa agatcttctt
     1201 gagatccttt ttttctacgc gtaatcttca tgactgtaaa cgaaaaaacc accctggcag
     1261 gtggtttttc gaaggttagt taatcctggc agattctcta accgtggtaa cagtcttgtg
     1321 cgagacatgt caccaaatct gtccttccag tgtagcctca gtcaggccgc cacttcaaga
     1381 actctgaaca catctctcgc acaaacctgt taccagtggc tgctgccagt ggcgttttgt
     1441 cgtgtctttc cgggttggac tcaagacgat agttaccgga taaggcgcag cagtcggact
     1501 gaacgggggg ttcgtgcata cagtccagct tggagcgaac tgcctacccg gaactgagtg
     1561 tcaggcgtgg aatgagacaa acgcggccat aacagcggaa tgacaccggt aaaccgaaag
     1621 gcaggaacag gagagcgcac gagggagcta ccagggggaa acgcctggga tctttatagt
     1681 cctgtcgggt ttcgccgcca ctgatttgag cgtcagattt tgtgatgctt gtcagggggg
     1741 cggagcctat ggaaaaacgg cagcgccgca tcctttttcc ggccttccct gacttgagtg
     1801 ccatctcttt atcttgtcat tcgtgagcag gtaacgcccg ccgcagcctc acggcaggag
     1861 cgtagcgacc gagtgagcga ggaggcgtca tatcaccgta actcccattt tctcccttac
     1921 gccctgtagc tgcattttcc cctgtcacat gaagcacttt cattaggtgc tactccgccg
     1981 taacatattc agccactgta cactccgcta gcgctacgtg actggttcag tgctgcgctc
     2041 cgaaacccgc taacaacctc tccgctacta gatatcaccc catgccatct gaatatgatg
     2101 aacaactgga ttcgactgat acgcagcttc ccggcgaata tgaccttttt gatttaatgg
     2161 cgcaggttac tgaagagaac ctgcacgccc cggtggattt tgggccgcct gtcggccggg
     2221 aagcgttata gctttttcgc ttacccgcgt acgtgtaata cgccggtatt acatcttatt
     2281 tctttatttt catgtgatta caggtggttg taatacgttg cgtattacat aaataaacgg
     2341 gaacttcagt ttttaccttt taattcatgc aattgcgatg aaaatcgcgg ttgcgtatgg
     2401 tgtatggcca tttaagggat aaagaatcat ctccttaccg ggctgagctt tgcctattct
     2461 gaggagatct gtgaaaaaaa gcgtccataa ctagctcgtt catacttgta tgaacagctt
     2521 attcctcctg actactaata aactacagcg tcactactga tttactacaa atcggaggag
     2581 gatgactact tatctactac tgatttacta ctgaattact actgaattac tactgaatta
     2641 cgtcataaca gttctctgaa ccattcgtac atccgacccc cttctcttca tgcttgatgc
     2701 tcttgccaac gcttcacggt cacgcaataa attgcgtccc cgttcaccgt tttcggatga
     2761 gaagaacgtc gaaaacagga cttccactgg ctgcacggcg tgaaattctg cgctatgcac
     2821 ttgcgcgcat actcatgcat gccgtaaaaa cagagcctgc gtgtttctgg cgggttttcg
     2881 ggtggtttgt tgccttgttt taccggtttc ccgtcagaaa cgctctcaga gcctctcagg
     2941 cggtgcacgc aacagatgtt atgttcaatc agactgatct gcgggtacaa aaaaagcccc
     3001 gggggctttt aactactgac ttattttttt gaggatttgc tgggtttcaa tttgtcagta
     3061 ttccccggcg tttttttatt gtcacgctgg cgtttatcag gttcccctgt tgaatcggca
     3121 attcgtttcg gacccggctt aagacctata atcgttatcc tttcaatagt taagaggaaa
     3181 gccctcaagc tggatatatt gacagcgtta cggattatca agaggggcca gcaagattat
     3241 tttttgttct tttaggttga ttgcgatact attgctatac atgtatatca aatggtgttc
     3301 aggaggttta tatgcttgct atccggttat ctgatgagat tgagtcccgt ctggactcgc
     3361 tggcgaagca aaccggcaga acaaagacgt tctatgcgcg ggaagcaata ctggcgcatc
     3421 tggaagacct ggaagattat tatctttcag cagaaactgc cgcacgcgtt cgccgtggtg
     3481 atgaagccgt gcattcgtct gaagacgtga ggaagtcact tggtctggac gattaactat
     3541 tccgaccggg cgctcaaatc gttacgcaag atggacaaac agaacgcacg acggattgtg
     3601 gattttatgg atttacgcat tgcagttgct gccgatcctc gccagtcagg gaagccgctc
     3661 aaaggtgagc tgggcgagtt ctggcgctat cgggtgggag attatcgcat tctgtgtgag
     3721 atccgggatg acgagctggt tatccttgcc gccaccattg gccatcgccg cgaagtttac
     3781 gactgaagcc cgctatgcgg gctttttttg atgctgataa cgccttttat gttcaagagc
     3841 tcctgatctg tctcggggca cccatgaagc ccgtggcatc cacctcgaac cggcaaaccc
     3901 cgatctgctg caggtagaga accagggtat cgagcttgcg aaaaactctg agctgcttgc
     3961 tgcgctgggc ggccagggca cgttcggcta gaccgtagcg caccagcagc gtccagccgc
     4021 caggttggcc aactgcgatc acgacacgaa cagcaccggc cgccaccagt tgtaccaggg
     4081 tgggatgggt aatggtttcc atgggggaga ttccgagaca agggatagtg cggcgcagga
     4141 tgggagataa cgcctagcaa aacaagtcat cttgcaggat gaccagcagc aaaaagggcg
     4201 tccgatttaa cgtaacgccc ccttatgcgc agtcatccag gcgggttatg actggggtgg
     4261 gtactgagca acgtagctgg ttgtcagctt caaaagactc gccaacacgg cacggaaaca
     4321 gcaccctttc cgtggcggca gacgcagtct gattggcaac gtgggcaatg aacgatgata
     4381 gtggccataa ggaatactga gtcttaaaag ccaccatggc tcacgttcat caacctactg
     4441 aaggcatcac caatttaatt ttggtgaaat aaattattct tttctcgata attacgtaag
     4501 ttgcataacc aataaatacg gacgacccaa ttaatataac cccaaaaaga aatgaatttg
     4561 tcacccctaa cttactagca ataattgcca tcggacttaa aacgaaagga tgggaaaggt
     4621 ataaggagta agagctatct ccaatccgtt caaataaatt tgagataaaa tgtttttttc
     4681 gtttaaaata cccctcaaat gataagaaac cataaaacag caaccccatt ggaacaccaa
     4741 tagatatcac tcttggagca cctgataaat actccatgtt aaagttttta tatagcaaaa
     4801 aagccaatgc caatgtaact gagatataac caaaattagc tttcaccaac cccctgtcat
     4861 aagcaacaaa taatataaca ccaatcgcaa actcatacag ccaactgctt aacaaaaaat
     4921 ccaccactat atcttctggc tgaaaaatca atccaaaaga ggataaaatt aagagagtag
     4981 caatgataca tgcatatctt agtttggttg acgagaaagc aaccaaaaat aacgaaaaga
     5041 ttagatagta ataaaattca tagcttaatg tccatccgtt atttaccaag tatttggcac
     5101 tagatggtat taaaaaatac gaatctatga tgctagtttc tccaccagat gagttaacaa
     5161 ggctgggaga tataacgaac acagtcagag ctagagtcgt aaatacccag taaagaggaa
     5221 tgattctctg aacacgcaac gtcaaaaaac ttatgattgt taaattttta ttatgtgttg
     5281 tgtagcacat tataaaacct gaaataataa aaaaaagatc gacccccgac ccgcctatat
     5341 gaaaccaact taaagtttct gtattgtaaa tatttccttt atgagcagta tgagccataa
     5401 cgacaagaag agcagcaata ccccgcaaaa actgtataga atataacatc gaccacctaa
     5461 ttccttacat tatagttcat ataacctaac catcttaagc aagtgttaga aatgggtact
     5521 taatggacac c
//
LOCUS       DAZPEB010000217         5433 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZPEB010000217 DAZPEB010000000
VERSION     DAZPEB010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5433
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.222"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            1..1037
                     /locus_tag="ACHURJ_003704"
                     /pseudo
     CDS             1..1037
                     /locus_tag="ACHURJ_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
     gene            complement(1077..2291)
                     /gene="ydcO"
                     /locus_tag="ACHURJ_003705"
     CDS             complement(1077..2291)
                     /gene="ydcO"
                     /locus_tag="ACHURJ_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFR1282479.1"
                     /translation="MLRCLYAITYEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVARATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAEILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTVASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLLAGLFGSAITEMMAALPVSWIQ
                     MLAGLALLSTIGGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIADRNQY"
     gene            2344..2880
                     /gene="sutR"
                     /locus_tag="ACHURJ_003706"
     CDS             2344..2880
                     /gene="sutR"
                     /locus_tag="ACHURJ_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFR1282480.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            2953..4914
                     /gene="rlhA"
                     /locus_tag="ACHURJ_003707"
     CDS             2953..4914
                     /gene="rlhA"
                     /locus_tag="ACHURJ_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFR1282481.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMNYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVMIKREVIGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVAEPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(5006..5236)
                     /locus_tag="ACHURJ_003708"
     CDS             complement(5006..5236)
                     /locus_tag="ACHURJ_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HFR1282482.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
BASE COUNT         1427 a         1391 c         1372 g         1243 t
ORIGIN      
        1 tcctggctgg ttgagtggaa aaatgccact gaaacgcaat ggcttaaaga ttctccctca
       61 cagccattgc aacagtcact gaaagacctt gagcgggctt acaaaaactt cttccggaag
      121 cgggctgctt ttccccgatt caaaaagcgg ggacagaatg atgcattccg ctacccgcag
      181 gttaagctcg atcaggaaaa cagccgtatt tttctgccga aactgggctg gatgcgctac
      241 cggaatagcc gtcaggtcac gggtgttgtg aaaaatgtca ctgtcagcca gtcctgcggt
      301 aagtggtaca tcagtattca gacagaaagt gaagtatcca ctccggctca cccttcagca
      361 tcaatggtcg gactggatgc tggcgtggct aaactcgcca cgctgtcaga tggcacagtc
      421 tttgagcctg taaacagttt tcagaaaaac cagaagacgc tggcgagact tcagcgacag
      481 ttaagccgca aggtcaaatt cagcaacaac tggcagaagc agaaacgcaa aatacagcga
      541 ctgcattcct gtatcgcaaa tatccgcagg gactaccttc acaaagtcac aacggccgtc
      601 agcaaaaacc acgcaatgat agtcattgag gatttgaagg tcagcaacat gtcaaagtca
      661 gcaggggtac ggtcagccag ccggggcgca atgtccgggc aaaatcaggt ttaaaccgtt
      721 cgatactgga tcagggctgg tatgaaatgc accgccagct tgagtacaag cagctctggc
      781 gtggcggtca ggtgcttgct gttccgccag cgtatacaag ccagcgttgc gcgtactgtg
      841 gtcatacagc gaaagagaac cgcctgtcac aaagtaaatt cagatgccag gtatgtggat
      901 atacagcgaa cgccgatgta aatggcgctc gtaacatttt agcggcgggg cacgccgttc
      961 ttgcctgtgg agagatggtg cagtcaggcc gcccgttgaa gcaggaaccc accgaaatga
     1021 ttcaggcgac agcctgaacg tagcagggat ccacgtcttt cagggcgtgt aggatgtcaa
     1081 tattggtttc tgtcagcgat taaattcaac accacgtaac aaacgcctcc ggcaattaat
     1141 ccccagaacg cagaaccaat cccgaccagc gtcaatccac ttgccgttac cagaaatgcc
     1201 accaccgccg cgtcgcgctc acgctcatta tgcagcgcct gatacaaact gccgccaatg
     1261 gtactcaaca gcgccagacc tgccagcatc tggatccaac ttacgggcag ggcagccatc
     1321 atctcggtaa tggcactacc aaacagacct gcgagcaaat agaaaatgcc tgcaacggca
     1381 gctgccagcc aacgttgatc tttatccgga tgcgcttccg ggctttggca aatagccgcg
     1441 gtgattgccg caataccgac ggaataaacg ccgaaagggg aaaaaaccag tgccagcaat
     1501 ccagtaaata caattaatgg cgaaacagga gccgaatatc cagctgcttt cattgctgcg
     1561 atacccggtg cgttttgcga tgccaccgtc accagaaaaa gggggagtgc aacgctcagg
     1621 ctgtgagcaa acgaaaaatc aggggtaata taagtgggga gaacgggttt aaagacaaca
     1681 tcagttgtga caacgtcacc ttgcgcgatg acgatcacga tcccaataat catcgcggca
     1741 attaccgcat agcgcggcgc aacggccttg gttgccagcc ataccagcaa catacttcca
     1801 cacaacgtaa attgaccgtc cagactggca aacgcctgta aaccaaagcg taataaaatc
     1861 tcggcaagca ttgccgccgc aagcgagtgc ggaataatgc gcatcagacg agcaaagagt
     1921 cccgttatgc cgcagaggac tattaacgcg ttggtgacaa taaaaacgcc gatggcttcg
     1981 ttaagtgtta gtccctgcaa tccggtgacc aacaaagccg cgccaggcgt tgaccatgcg
     2041 gtgagaacag gtacgcgata ccataatgtc agagtcagcg tactgacgcc cattgccagc
     2101 cccagcgccg tcatccagcc agagatttgt gcagtggtgg ctctggcgac aatcgctgct
     2161 tgccagatta ttgccgctga actggcgtag ccaattaata ccgccagaaa ccccgccagt
     2221 agcgtgggtg gaggaataga aaacagacgc atagtcacct cgtacgttat agcgtacagg
     2281 caccgtagca tttgtccgtt ataacgcaca agtgataaac ttccgttttg ccggaggagt
     2341 cgcatggaaa atctcgctcg ctttttatcc accacactta aacaactacg ccagcagcgc
     2401 ggctggagtc tttcgcgact ggcagaagcg acgggcgttt ctaaagcaat gttagggcag
     2461 attgagcgta atgagtccag tccgacggtc gcgacattat ggaaaattgc caccgggttg
     2521 aacgtaccat tttcaacatt tatttctccg ccgcagtccg ctacgccttc agtttatgat
     2581 ccacaacagc aggcgatggt gatcacttcg ctgtttcctt atgatccaca actctgcttt
     2641 gaacacttct cgattcagat ggcatctggt gcaataagcg aatcgacgcc ccatgagaag
     2701 ggggtaatcg aacatgtcgt cgtcattgat ggacaacttg atctgtgcgt tgatggggaa
     2761 tggcagaccc ttaattgtgg ggaaggcgtt cgatttgctg cagacgtcac gcatatctat
     2821 cgtaatggtg gggagcaaac cgtacatttt cattccctca tccattaccc gcgcagttaa
     2881 gcgggaaaac tatttcgcaa cgtcgcgctt gtggctaaaa tagccgccat ttttcagcta
     2941 ctggataaga atgtgaccgt atcttctcat cgacttgaac tgttaagccc ggcacgcgat
     3001 gccgccattg cccgcgaagc tattttgcac ggtgccgatg ctgtttatat cggcggccct
     3061 ggttttggtg cccgtcataa tgccagtaat agcctgaaag atattgccga gctggtgccg
     3121 tttgcccatc gttatggtgc aaaaattttc gtcacgctta acaccatttt gcatgatgat
     3181 gagctggaac ccgcgcaacg gctgattact gacctctacc agaccggtgt cgatgcgctg
     3241 attgttcagg atatggggat tctggaactt gatattccgc cgattgaact gcacgccagt
     3301 acgcagtgcg acattcgtac agttgaaaaa gcgaagttcc tctctgatgt tggcttcacg
     3361 cagattgtgc tggcgcgaga gctgaatctt gatcagatcc gcgcgattca ccaggctacg
     3421 gacgcgacca ttgaattctt tattcatggg gcactgtgcg tggcctattc gggtcagtgc
     3481 tacatttctc atgcgcaaac agggcgtagc gccaaccgtg gcgattgctc gcaggcgtgc
     3541 cgtttgccat acacattgaa agacgatcag gggcgggtgg tttcctatga aaaacatctg
     3601 ctgtcgatga aagataacga tcagactgcc aacctcggcg cgctgattga tgctggtgta
     3661 cgctccttca agattgaagg tcgttacaaa gatatgaact acgtgaagaa tatcaccgcc
     3721 cattatcgcc agatgcttga tgccattatt gaagaacgtg gcgatctggc gcgcgcttca
     3781 tcaggtcgta ctgaacattt ctttgttcca tcgacggaaa agactttcca ccgtggtagc
     3841 acagattatt ttgtgaatgc ccgtaaaggc gatattggcg cgttcgattc gccgaaattt
     3901 atcggcctgc cggtgggtga agtattgaaa gtggcgaaag atcatctcga tgttgccgtc
     3961 accgagccac tggcaaatgg tgatggcctg aacgtgatga ttaaacgtga agtcatcggt
     4021 tttcgtgcca atacggtcga gaaaaccgga gaaaatcagt accgcgtctg gcccaatgaa
     4081 atgccagcag atttgcacaa aattcgtcca catcacccac taaaccgtaa tcttgatcat
     4141 aactggcagc aggcactgac aaaaacctcc agcgaacgtc gggtggcggt agacattgaa
     4201 ctgggcggct ggcaggaaca actgattctg accctcacca gtgaagaggg tgtcagcatc
     4261 acgcatacgc tggacgggca gttcgacgaa gccaataacg ccgaaaaagc aatgaacaat
     4321 ctgaaggatg gtctggcaaa actggggcaa accctctatt acgcccgcga tgtgcaaatt
     4381 aatttgccgg gggcgctgtt tgtaccaaac agtctgttaa accagttccg ccgtgaagct
     4441 gctgacatgc tggatgctgc gcgtcttgcc agttaccagc gcggcagccg taaaccggtt
     4501 gctgagcctg cgccggttta tccgcaaacg catctgagtt tcctcgcgaa cgtatacaac
     4561 cagaaagcgc gtgaatttta tcatcgctat ggtgtgcagc tgattgacgc ggcgtatgag
     4621 gcacatgaag agaagggcga agtcccggtg atgatcacca agcattgtct gcgctttgcc
     4681 tttaatctgt gcccgaaaca ggcgaaaggc aatatcaaaa gctggaaggc gacgccaatg
     4741 caactggtta acggcgatga agtattaacg ctaaagtttg attgccgccc atgcgagatg
     4801 cacgtcattg gcaaaatcaa aaatcacata ctgaaaatgc cgttaccggg aagcgtagtg
     4861 gcatccgtaa gtccggatga gctgctgaaa acattgccta agcgaaaagg gtaaaacgcc
     4921 agttttctgg ttactcacaa ctcattgaat ctgcatgata ttgcctgccg ggtaaggcgt
     4981 cacgccgcat ccggcatcaa acgactcaac gttgtcccgc ttctggtttg cgcgattttt
     5041 gccagtaaaa atgctcgcgc aaaccttccg ccgactcttc cgccacagca cgcaattcat
     5101 cgctgtccgc ttcatgacgc agctgatgat ccacattctt tacccacaca aattcatgtc
     5161 ctttgtgccc tgccatgagt tgtcctgaga acagcgcaca cgttaataag acaaccgata
     5221 acgccttcgt aaacatcctg ccaccttttt cttacctttt gccgctatga tgccgatcgt
     5281 ttcttgaggt tattattcag ttttgcaaat tagcgcaaag aaattctgga atcttccttc
     5341 ctgattttgc attgcattct gtcgttgcgg cgatttagtg ctattttcga gcaaattaca
     5401 cacagaggta aacactaatg tttactttgt tga
//
LOCUS       DAZPEB010000218         5374 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZPEB010000218 DAZPEB010000000
VERSION     DAZPEB010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5374
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.223"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            1..320
                     /locus_tag="ACHURJ_003709"
                     /pseudo
     CDS             1..320
                     /locus_tag="ACHURJ_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(708..1084)
                     /gene="rnpB"
                     /locus_tag="ACHURJ_003710"
     ncRNA           complement(708..1084)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHURJ_003710"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1117..2262)
                     /gene="garK"
                     /locus_tag="ACHURJ_003711"
     CDS             complement(1117..2262)
                     /gene="garK"
                     /locus_tag="ACHURJ_003711"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFR1282483.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYAEVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(2359..3249)
                     /gene="garR"
                     /locus_tag="ACHURJ_003712"
     CDS             complement(2359..3249)
                     /gene="garR"
                     /locus_tag="ACHURJ_003712"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFR1282484.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(3279..4049)
                     /gene="garL"
                     /locus_tag="ACHURJ_003713"
     CDS             complement(3279..4049)
                     /gene="garL"
                     /locus_tag="ACHURJ_003713"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFR1282485.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(4065..>5374)
                     /gene="garP"
                     /locus_tag="ACHURJ_003714"
     CDS             complement(4065..>5374)
                     /gene="garP"
                     /locus_tag="ACHURJ_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFR1282486.1"
                     /translation="TVDVKKKGVHTRYLILLIIFIVTAVNYADCATLSIAGTEVAKEL
                     QLSTVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGFVDMF
                     PLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFSLALF
                     SPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIYNPTDHPRMSAEELKFISENGAV
                     VDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIYLVQE
                     KGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLLASTI
                     ILCNYTNNTTLVVMLMALAFFGLGWPVISDTAPKEIVGLCGGVFNVFGNVASIVTPLV
                     IGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
BASE COUNT         1361 a         1509 c         1254 g         1250 t
ORIGIN      
        1 gcttgaacag aattaaatta ccagagatga aagaattctc atcaagaaaa aacatatgat
       61 tacatcgata taatcattga agattatgaa aatatcaaag aaaatgctta tctggtcgtc
      121 ccccaaatta attatactat ggatttaaac atagaagact ccagctcaga agagttaata
      181 ccctcgagaa agacgaaaat tcttcggaca atggctttga agtcgaagaa tataacgcat
      241 atgaagcata taactcagag aatctatatt ttaccagaga ggaatatacg tatgattacg
      301 acctttcaaa tgcaatatag acttaataac ttcttacaaa atctataatt aataccgtag
      361 atattcattc atcttaccct gtgcaccaaa agcaaaaact cactgacgcg ggtttgaaac
      421 tgatggcaac cgcaaaaatg cctgatgcgc tacgcttatc aggcctacgc catctctgca
      481 atatattgaa tttgcgtgct tttgtaggca ggataaggcg ttcacgccgc atccggcatg
      541 aataaagcgc actcaacaat ctgaaacccg ccggaacggt ttattacgta catcaggtga
      601 aactgaccga taagccgctt tcttttgggt atagtgtcgt ggacagtcat tcatctttct
      661 gcccctccaa aagcaaaaac ccgccgaagc gggtttttac gtaaatcagg tgaaactgac
      721 cgataagccg ggttctgtcg tggacagtca ttcatctagg ccagcaatcg ctcactggct
      781 caagcagcct acccgggttc agtacgggcc gtaccttatg aacccctatt tggccttgct
      841 ccgggtggag tttaccgtgc cacggactgt taccagccgc gcggtgcgct cttaccgcac
      901 cctttcaccc ttacctgatc ccgcttgcgc gggccatcgg cggtttgctc tctgttgcac
      961 tggtcgtggg tttccccccc aggcgttacc tggcaccctg ccctatggag cccggacttt
     1021 cctcccctcc gcccgtctcc cccgaagagg acgacgacga agcggcgact gtctggtcag
     1081 cttcggcgcg cagtatagag ggtttgcgcg cccttgtcac cccgcgttgc gcattccaat
     1141 cgccagtgtc gcggcgatat tacgtgaagc acggcagata ttgtcataag ccccgcggaa
     1201 tgcttcgtcc aacgtaccta tgctggtcaa tacgctgaag accgcatcaa tgccatgctg
     1261 atgtacaacg ccaacatcat cggtcaggct acccgcaatg ccaatcaccg gtttatggta
     1321 cttcttcgcc acgtttgcga caccaatcgg taccttgccg tgaatactct ggctgtcaat
     1381 acgcccttca ccggtgatca ccagcgtaca atcgtgaata tgttcttcca gattcagcgc
     1441 cgtagtgacg atttcaatac cacttttcag ttccgcacca agaaacgcca tcagcgccgc
     1501 gcccataccg cccgcagctc ctgcgccggg gacatctttc acatcaacat gcagcgcttt
     1561 tttaatgacc tcggcatagt gagagaggtt attgtccagc tcaacaatca tcgcttcact
     1621 ggctcccttt tgtgggccaa agatgcgcga tgcgccgtta tcgcccacca gcggattggt
     1681 gacatcacaa gcgacgcgaa tgacgcaatc ttttaagcgc ggatcgaggc cggaaatatc
     1741 aatatcattc agagtattaa gactaccgcc gccaaaacca atttcattgc cgttggcgtc
     1801 gcataatttc gcccccagcg cctgtaccat gcctgcgccg ccatcatttg tagcgctgcc
     1861 gccaatgccg ataataatgt ttgtcgcacc gctctccagc gcctgcagga ttaactcgcc
     1921 tgtgccgcgt gaagtggtca cgagtggatc gcgtttttcc gcaggtacca gctccagccc
     1981 actggccgcc gccatttcaa taaacgcggt tttgccatcg ccggagatcc cccaactggc
     2041 attcactttc tcgcccaacg gccctgtaac ccaggcgtga cgttcggaac cctgggtggc
     2101 tgcaatcatc gcttccaccg ttccttcgcc accgtcggca accggaacag aaacgtactg
     2161 tgcatcagga aaaatttccc gaaatccttt ttctatcgcc tgcgcaacct cgctggcaga
     2221 taaactttct ttataagagt ctggggcgat tacgattttc atacctatgc ctgttaccac
     2281 atgacgccgg agggcgtttc tcttattcgg cgtggattcc aggcccggat tgcaatacgc
     2341 catccgggca cgacgtcatt aacgagtaac ttcgactttc gccagttttt cgtagtagca
     2401 cgccagagcg ctatgatccg ccgttcctaa accatctgca cgcagtgcct gcatcatctc
     2461 cataaccgca gctgtgagcg gcagttgcgc gccgacgccg tgagaagtat ccagcgcatt
     2521 cgccagatcc ttaatatgca gatcaatgcg gaagcccggc ttgaagttgc ggtccatcac
     2581 catcggcgct ttggcatcca gcacggtact gcccgccagc ccaccgcgaa ttgcctgata
     2641 aaccaggtcc gggttaacgc ccgctttagt tgccagcgtt aacgcttctg acatcgcagc
     2701 aatattcagc gccacaatga cctgatttgc cagtttggtg acgttacctg caccgatttc
     2761 cccggtatgc accacggaac ccgccatcgc tttcatcaaa tcatagtatt tgtcgaaaat
     2821 agccttgtcg ccgcccacca tcactgacag cgtaccgtcg atggctttcg gttcaccgcc
     2881 gctcaccgga gcatccagca tatcaatgcc tttcgctttc agcgcttcgc tgatttcacg
     2941 gcttgccagc ggtgcgatag aactcatatc gatcaatacc gtacctggct tcgcgccttc
     3001 aataatgcca ttctcaccca gcgccacctc tttcacatga ggggagtttg gcagcatggt
     3061 tatgatgacg tcgcactgtt cagcgatcgc tttagccgta gacgctgttt ctgcacctgc
     3121 agcaatcacg tcagcaatag cttctgggtt acggtcagca accaccagcg agtaacctgc
     3181 tttcagaagg tttttactca ttggtttacc cataatcccc aggccaataa aaccaacttt
     3241 catagtcata tcaatcatct ctcttgttgc ggtggtggtt attttttaaa ggtatcagcc
     3301 agtttctgag tggcagagcg gaagacgcca agatcgctgc cgacagccac aaacgtcgcg
     3361 ccccattcca gataacgacg cgcatcggct tcgaccggcg cgagaatacc gctgggtttg
     3421 ccgtgcgcac tggcacggtt aaaaatatgc tgaattgctt tttgtacatc cggatgcgag
     3481 gcattgccga gatggcccaa tgccgcagcc agatcgctgg ggccgacgaa gataccgtct
     3541 acaccttcgg tagcggcaat agcatcgacg ttatctacgc cctgctgact ttctatctgg
     3601 accagaatag tgatgttctt gttcgactga gcgaaataat ccgccacggt gccaaacata
     3661 ttggcgcggt gagaaacgga gacgccgcga atgccttccg gcgggtaacg agttgatgcc
     3721 accgcctgct ctgcttcctc ttttgtttct acaaaaggaa tcaggaaatt atagaaaccg
     3781 atatccagaa gacgcttaat aattaccggc tcgttggtcg gcactcgcac tactggcgcg
     3841 ctggcgctgc ctttcagggc cattaactgc ggaataaacg tggaaatatc gtttggcgca
     3901 tgttcgccat ccagcaccag ccagtcaaac ccagccaaac caagaacttc agtgctaatc
     3961 gggttagaga gtgctgacca gcaaccaatt tgtacctgtt tcgcagccag tgcggctttg
     4021 aatttattcg ggaaaacatc gttattcatc gcttatacct ttgcttattt ctgcaattcc
     4081 atacgtttaa tgtcgccaac tacgaagagg tagcagacca tcgccatcag cgctgaacat
     4141 cccacgaaaa ccagtgctgc attgaaggag tgcagttcac ttaccaggta gccaatcacc
     4201 agtggagtga caatggaggc aacattgcca aagacgttaa agacgccgcc acagaggcca
     4261 acaatctctt tcggcgcggt gtcagaaatc accggccagc ccagaccaaa aaaagccagc
     4321 gccatcagca tgaccaccag cgtggtgttg ttggtgtagt tacataagat gatggtggaa
     4381 gccagcaaca ttcccagcac aatcggtagc ttacgtgcca gggtcaggga taaaccgcgt
     4441 ttgatcagat aatccgagaa gacacctccc agcacgccgc ccgcaaaacc acacagtgcc
     4501 ggaatcgagg cgaccagacc cactttcaga atcgacatgc ctttttcctg caccagataa
     4561 atcgggaacc aggtgaggaa gaaccaggtg atggtgttga taaaatattg tccgaaaaat
     4621 acgcccaaca tcatgcggtt agagagcaat tgcttgatgt aatgcagttt gggaccgctt
     4681 gctgccgcgc tgcccggctt tttgtggtcc atatcgacca ccgcgccatt ttcagagata
     4741 aacttcagct cttccgcaga catacgtggg tgatctgtcg ggttataaat caacttgatc
     4801 cacagcgccg tcagcacaaa accaatcacc cccataacgg tgaagacgtg ctcccagccc
     4861 caggcgaaag tcagccagcc aagcagaggc gaaaagagcg ccagcgagaa atattgcgcc
     4921 gagttaaaga tggcggaggc agtaccacgt tctttcgtcg ggaaccaggc ggcgacaata
     4981 cgggcgttcg ccgggaatga tggcgcttcc gagaagccga gcataaagcg cataaagaac
     5041 atagagatcc ctgcccaggc cagcgggaac atatcaacaa agccttgcag gaaggtgaat
     5101 agcgaccaga aaaagaggct gtaggtgtaa acttttttcg agccaaactt atcaagcagc
     5161 cagccgccgg gaatttgcat cagcaggtag gcccagccaa aagcggagaa gatgtaaccc
     5221 atcgaaaccg tacttaactg caactctttt gccacttcgg taccagcaat agacagcgtt
     5281 gcacaatcgg cgtagttaac ggcggtaaca ataaaaataa tcagtaatat taaatagcgg
     5341 gtatgcacgc ctttcttttt tacgtcaacg gtgt
//
LOCUS       DAZPEB010000219         5348 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZPEB010000219 DAZPEB010000000
VERSION     DAZPEB010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5348
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.224"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(55..1338)
                     /gene="aroA"
                     /locus_tag="ACHURJ_003715"
     CDS             complement(55..1338)
                     /gene="aroA"
                     /locus_tag="ACHURJ_003715"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFR1282487.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1409..2497)
                     /gene="serC"
                     /locus_tag="ACHURJ_003716"
     CDS             complement(1409..2497)
                     /gene="serC"
                     /locus_tag="ACHURJ_003716"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFR1282488.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSIFNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2696..3388)
                     /locus_tag="ACHURJ_003717"
     CDS             complement(2696..3388)
                     /locus_tag="ACHURJ_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFR1282489.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            3518..5278
                     /gene="ycaO"
                     /locus_tag="ACHURJ_003718"
     CDS             3518..5278
                     /gene="ycaO"
                     /locus_tag="ACHURJ_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFR1282490.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         1368 a         1376 c         1266 g         1338 t
ORIGIN      
        1 atgtgccgac gttttgataa agaaagattt ggctatttat tgcccgttgt tcattcaggc
       61 tgcctggcta atccgcgcca actgctcgaa ataatccgga aatgttttgg ccgtacattt
      121 gggatcaaga atcgtcactg gtgtatctga caacgccacc agcgagaaac acatcgccat
      181 ccggtgatca ttgtatgtcg cgatctcggc aaagttcagt ttttccggtg gagtgatacg
      241 aatgtaatcg tgcccctctt ctacttccgc accgacttta cgcagttctg ttgccatcgc
      301 aaacaggcga tcggtttctt taacacgcca gttatagata ttgcgcagcg tggtggtgcc
      361 ttttgcaaat aacgccgccg tggcaatggt catcgccgca tcgggaatat ggttcatatc
      421 catatcaata gcgttcagtt caccacgcgt gcaggaaata taatcatcgc cccagcaaat
      481 ggtcgcgccc attttttcca gcacatcagc aaagcgaata tcaccctgca tactgttacg
      541 tccaataccg gtcactttta cagtgccgcc tttgattgct gctgctgcca gaaagtaaga
      601 agccgaagat gcatcgcctt cgaccaaata agtacccgga gactgataag actgtccgcc
      661 ttttacgaca aattgttgat agtgctgatt ttcaatttca acaccaaacg tcttcatcag
      721 attgagtgtg atgtcgatat aaggtttaga aaccagatcg cctttaatac gaatcaccgt
      781 atcttccggc gcaagaggcg cagtcattaa cagtgcggtg aggaattggc tggaaacgga
      841 gccatcaacg tcaacgttgc cgccagtaaa gccgccctgt aaacgcaacg gcggataatt
      901 ttcttgttcc aggtaagtga tcttcgcccc gcccaggcgc agcgcatcca ccagatgacc
      961 aatcgggcgt tctttcatac gcggctcacc ggtcagcaca atatcattgc tacccagaca
     1021 aagagctgcc gccagcggac gcattgccgt tccggcgtta ccgaggaaca actccagggc
     1081 accttctgcg tgtaatggac cgccgttacc gataatttcg caacgcgtac gatcggctga
     1141 aagcgtatag cttaccccta acgctgttaa tgcattcagc atatggcgca cgtcatcgct
     1201 atccagcaga ttggttaata ctgttttgcc gtgtgctaat gccgccagca ataaagcgcg
     1261 gttagaaacg ctcttggaac cgggcagatt aatagtgcca tcgacacgag cgatgggttg
     1321 taacgtcagg gattccatga actcaactct ctacaacaga aataaaaacc ccacaggctg
     1381 gctgtgggga ttaagcaaaa tttcggcatt aaccgtgacg gcgttcgaac tcaaccatga
     1441 agtctgtcag cgctttaacg ccttccagcg gcatggcgtt ataaatagaa gcgcgcattc
     1501 cgccgaccac acggtgacct ttcagtgcat gaaggccagc agcaaaagac tcttcaagga
     1561 acaatttgtc aagcgcactg tccgccaact ggaacggcac gttcatccgc gaacggttag
     1621 ctttcgccac gtcattgcgg tagaaatcgc tgttatcaat caccccatat agcagttctg
     1681 ctttttgctg attgatttta tccatttcag ctacaccgcc gttcgctttc agccatttaa
     1741 agaccagacc agatagatac caggcaaatg tcggcggcgt gttaaacatg gagccgttat
     1801 cgttgaagat ggaataatcc agtatcgacg gacacgcgat attcgctttg cccagcaaat
     1861 cttcacgaac gatgacgatt gtcaggccag ccgggccgat atttttctgc gcgccagcgt
     1921 aaatcacgcc ataacggctg acgtcaatcg gacgggaaag aatggttgaa gagaagtcgg
     1981 cggcgaccac cacatctttg ccgaagtctg gcgtttcgtc gatggcgata ccgtcgatgg
     2041 tttcattcgg gcagtaatgc atataagcag cattatcaga gagttgccat tcacgcattg
     2101 gcttaaccgc gcgcagacca tcaacagtca ctttggcgtc aaagacatta ggcgtgcagt
     2161 attttttcgc ttctttaatg gcacttgccg cccagtaacc ggcatcaaca taatctgcgg
     2221 tggttttatc accgagaata ttcagcggta ccgcagcaaa ctgaccgcga ccaccgccat
     2281 ggcagaataa taccttgtag ttggagggga cattaagaag atcgcgaaaa tccttctcgg
     2341 cttcctctgc aacctgaatg aactctttgc cacggtgact cacttccatc accgacgtac
     2401 caagaccgtt ccagtcgcgc agttcctgtt gagcctgttt aagcacctct gccggtagca
     2461 ttgccggacc agaactaaaa ttgaagattt gagccatttc ccctcaccac gttgcgttgc
     2521 tattaacccg cggtcaatcg attgcgacca caggtaatac attattcctg tggctatcgg
     2581 ttttatcatt cagtgacaca catcgcaatg agtaaaaccg ttgagggcaa aaatgaggct
     2641 tgcatcacac aaaagaatct ctcggattat tagcaaaaag ccgatgtttt tactgtcagg
     2701 tcacccgttt tgcccgactt gccttcgtcc attctggatt tgcacagcgc ggacataatt
     2761 gtttttcccc cgctttcata tgaatgattt cactacaacg aatacaggca tagtccgccg
     2821 actccggcac cactttgtaa cgctgcgtac aatcactggg taaaataagt aaacccggtt
     2881 tcaccttgtc atcttcaaag aaatagacac tgatttgccc gttggtttcg agaatcgcca
     2941 gacgtacctg ccccagctgc tccacgccac gcaatcgtag ctccataaag aactcaaatt
     3001 ccgtcatgtt ggagttattg agtttcgacc aggccagctc gccatcttca ataataacaa
     3061 ccggcttacc ttccagtaga tcttccagtt tttcactgtg cgccatcaac cacattacca
     3121 ggcggtataa caacgccaga gtgataaaga cgataagtac cggcaccatc ggcacatcat
     3181 cataaaacgc cacatctccc gccgctgaac ccagcgtgag aatgattaac acttcaaaca
     3241 gcgacatctg tcgcacaccg cgtcttccgg tcattttgag gaacaaaaaa accagtacaa
     3301 aggtataaag actacgtagt gcaacttcgc caaggaaatc aaaaggcact ttatcaaatg
     3361 ccatacggtg gagatcgaac gccttcattt ttatgctctt atcgtggttt attcgctaaa
     3421 agcatagtca acagttcatc ggcatggcgg cggtgatagc gcctttgaga taaaacccgt
     3481 attattgcgc gctttccgta cgactaaagt gattttcatg acgcaaacat ttatccccgg
     3541 caaagatgcc gctctggaag attccatcgc tcgcttccag caaaaacttt cagacctcgg
     3601 ctttcagatt gaagaggcct cctggctgaa tcctgtgcct aacgtctggt ctgtacatat
     3661 tcgcgacaaa gagtgcgcac tgtgttttac caacggtaaa ggcgcaacca aaaaagcggc
     3721 gctggcttct gcacttggtg aatatttcga gcgtctctca accaactact tttttgctga
     3781 tttctggctg ggcgaaacca tcgccaacgg tccgttcgtg cattatccca acgaaaaatg
     3841 gttcccactg accgaaaatg acgatgtgcc agaaggacta ctcgatgacc gtctgcgcgc
     3901 gttttatgat ccggagaatg aactgaccgg cagcatgctg attgacctac aatccggtaa
     3961 cgaagatcgt ggtatttgcg gcctgccgtt tacgcgtcag tccgacaatc agaccgttta
     4021 tattccgatg aatatcattg gtaacctgta cgtctccaac ggtatgtccg caggtaatac
     4081 ccgcaacgaa gcacgcgttc agggattgtc tgaagttttc gaacgctacg tgaaaaaccg
     4141 cattattgct gaaagcatca gcctgccaga gatcccggca gacgtgctgg cgcgttaccc
     4201 agcggtggtt gaagcgatcg aaacactgga agcagaaggt ttcccgatct tcgcatatga
     4261 tggttccctt ggcggccagt atccggtgat ttgcgtggta ctgttcaatc ctgctaacgg
     4321 tacctgcttt gcctctttcg gtgcgcatcc tgattttggc gtagcactgg aacgtaccgt
     4381 gaccgagctg ctgcaaggtc gtggcctgaa agatttggat gtgtttactc cgccaacctt
     4441 cgatgatgaa gaagtcgctg aacataccaa cctcgaaacg cactttatcg attccagcgg
     4501 tttaatctcc tgggacctgt tcaagcagga tgccgattat ccgtttgtgg actggaattt
     4561 ctccggcacc acggaagaag agtttgctac gctgatggct atcttcaaca aagaagataa
     4621 agaagtttat attgccgatt acgagcatct gggcgtttat gcttgccgta ttatcgtgcc
     4681 tggcatgtcc gatatttatc cggctgaaga tctgtggctc gcgaataaca gtatgggcag
     4741 ccatttacgt gaaacgattc tttcgctacc aggcagcgag tgggaaaaag aagattacct
     4801 gaacctcatc gagcaactgg atgaagaagg ttttgatgac tttacccgcg tgcgtgagct
     4861 gttgggtctg gcgaccgggt cggataacgg ttggtacacc ctgcgtatcg gtgaattaaa
     4921 agccatgctg gcgctggctg gtggcgatct ggaacaggct ctggtctgga ccgaatggac
     4981 gatggagttt aactcatcag tatttagtcc ggaacgcgcc aactattatc gctgcctgca
     5041 aacgttgtta ttactggcac aggaagaaga tcgccagccg ctgcaatatc tgaatgcgtt
     5101 tgttcgcatg tacggcgcag atgccgtgga agccgccagt gcggcaatga gcggcgaagc
     5161 ggcgttttac ggcctgcaac cggtagatag cgatctgcac gcgtttgctg cacatcagtc
     5221 gttgttgaag gcctacgaaa agctgcagcg cgccaaagca gcattctggg caaaataaaa
     5281 tcaaatagcc tacgcaatgt aggcttaatg attagtctga gttatattac ggggcgtttt
     5341 tttaatgc
//
LOCUS       DAZPEB010000220         5323 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZPEB010000220 DAZPEB010000000
VERSION     DAZPEB010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5323
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.225"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..3160
                     /gene="sigA"
                     /locus_tag="ACHURJ_003719"
     CDS             <1..3160
                     /gene="sigA"
                     /locus_tag="ACHURJ_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HFR1282491.1"
                     /translation="LDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHGV
                     TSTVGFISSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVSG
                     GGELTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGDN
                     LHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSESG
                     NGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGNV
                     SGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGNN
                     NCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNLE
                     NATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSAG
                     ESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIEL
                     AGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILFG
                     VDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQSL
                     KTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGSN
                     NSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEHT
                     TWTLTGYKVAENTASSSAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEVGAWARIM
                     SGAGSASGGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSVG
                     AGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHVT
                     EDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDWK
                     VTARAGLGYQFDLFANGETVLSDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLEF
                     EKSAFGKYNVDNAINANFRYSF"
     gene            3499..3789
                     /locus_tag="ACHURJ_003720"
     CDS             3499..3789
                     /locus_tag="ACHURJ_003720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282492.1"
                     /translation="MLVTDTITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKN
                     MTLRLRLQDQQEETSVTLFQGKMSTDGNNYYIIKGKVSSVLLLLKMLQINHA"
     gene            complement(4192..4592)
                     /locus_tag="ACHURJ_003721"
                     /pseudo
     CDS             complement(4192..4592)
                     /locus_tag="ACHURJ_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4596..5171
                     /locus_tag="ACHURJ_003722"
                     /pseudo
     CDS             4596..5171
                     /locus_tag="ACHURJ_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5222..5323
                     /locus_tag="ACHURJ_003723"
                     /pseudo
     CDS             5222..5323
                     /locus_tag="ACHURJ_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1593 a         1149 c         1262 g         1319 t
ORIGIN      
        1 tctggactgg aaaaacaaga gttacattta taccaacaga acccctttta aaaactcacc
       61 aatttttggc gatagtggtt ctggttctta tctatatgat aaagaacaac aaaaatgggt
      121 tttccatggt gttaccagta cagttggttt tatcagtagt accaatatag cctggacaaa
      181 ctactcgtta tttaataata ttctggtaaa caatttaaaa aagaatttca caaacactat
      241 gcagctggat ggtaaaaaac aagagttatc atcgattata aaagataagg acctgtctgt
      301 ctcaggagga ggggaattaa cgctcaagca ggataccgat cttggcattg gcgggcttat
      361 attcgataag aaccagacat ataaagtgta cggaaaagat aagtcttata aaggtgccgg
      421 gatagatatt gataataata ccaccgttga atggaatgtt aagggcgttg ccggagataa
      481 tctgcataaa ataggtagtg gtactctgga tgtaaaaata gcacagggaa ataaccttaa
      541 aataggtaat gggactgtca tccttagtgc tgaaaaagcc ttcaataaaa tttacatggc
      601 cggaggtaaa ggtacggtaa aaataaatgc caaagacgct ttaagcgaaa gcggtaatgg
      661 cgaaatctat tttaccagaa atggcggaac actggatcta aacggctatg accagtcatt
      721 tcagaaaatc gcagcaacag atgcgggaac aaccgtaacg aactcaaacg tgaagcaatc
      781 aacattatca cttactaata ctgatgcata tatgtaccat gggaatgtat caggtaatat
      841 aagcataaat catattatca atactaccca gcaacataac aataatgcca atctgatctt
      901 tgatggctca gtcgatatca aaaacgatat ctctgtccgg aatgcacagt taacattaca
      961 aggacatgcg acagaacatg ccatatttaa agaaggcaat aacaactgtc caattccttt
     1021 tttatgtcaa aaagactatt ctgctgccat aaaggaccag gaaagcactg taaataaacg
     1081 ttacaatacg gaatataagt ccaacaatca gatagcctct ttttcccagc ccgactggga
     1141 aagtcgtaaa tttaatttcc ggaaattaaa tttagaaaac gcaaccctga gtataggccg
     1201 ggatgctaat gtaaaaggac acatagaggc taaaaactct caaattgttc tgggaaataa
     1261 aactgcatac attgacatgt tctcaggaag aaacattact ggcgaaggtt ttggattcag
     1321 acaacagctt cgctccgggg attcagcagg cgaaagtagt ttcaacggca gtctgagtgc
     1381 tcaaaacagc aaaataactg ttggtgataa atcaactgtt actatgactg gtgcattatc
     1441 cttaattaat acagacctga ttatcaacaa aggagctact gttaccgccc agggaaaaat
     1501 gtatgtagat aaagctattg aactggccgg aaccctgaca ttaacaggca cccctacaga
     1561 aaataataaa tacagcccgg caatctatat gtcagatgga tataatatga cagaagatgg
     1621 tgccacgtta aaggctcaaa attatgcctg ggtcaatggt aatataaaat cagacaaaaa
     1681 agcatctatt ctgtttggtg ttgaccagta taaagaagat aacctggaca aaaccacaca
     1741 cacaccgctg gctacaggtt tgctgggtgg ctttgatact tcttataccg gaggtattga
     1801 tgctcctgct gcctcagcca gcatgtataa caccttatgg agagtaaacg gacagtcagc
     1861 cctgcaatca ttaaaaaccc gcgacagtct tttgttgttt agtaacatag agaattcggg
     1921 tttccatact gtgacagtaa acacactgga tgccactaat actgctgtga ttatgcgggc
     1981 tgatctgagc cagtctgtaa atcaatcgga taaactcatt gttaaaaatc agttaaccgg
     2041 aagcaataac agtctgtcgg tcgatataca gaaagtggga aataataact caggattaaa
     2101 cgttgacctg ataacagccc caaaaggaag caataaagag atatttaaag ccagtactca
     2161 ggccataggt ttcagcaaca tatctcctgt gatcagcacg aaagaggatc aggaacatac
     2221 cacgtggacc ctgaccggat ataaggtggc tgaaaataca gcatcttcca gtgcagcaaa
     2281 atcgtatatg tccggtaatt acaaagcctt cctgacagaa gtcaacaacc tgaataaacg
     2341 aatgggggat ctgcgtgaca ccaatggcga ggtcggtgca tgggcccgca tcatgagcgg
     2401 agcaggttca gcttctggtg gatacagtga caactacacc catgtgcaga ttggtgtgga
     2461 taaaaaacat gagctggatg gacttgacct tttcactggt ctgactatga cgtataccga
     2521 cagtcatgcc agcagtaatg cattcagtgg caagacgaag tccgtcgggg caggtctgta
     2581 tgcttccgct atatttgact ctggtgccta tatcgacctg attagtaagt atgttcacca
     2641 tgataatgag tactcagcga cctttgctgg gctcggaaca aaagactaca gttctcattc
     2701 cttgtatgtg ggtgctgaag caggctaccg ctatcatgta acagaagact cctggattga
     2761 gccgcaggca gaactggttt atggggccgt atcaggtaaa cggttcgact ggcaggatcg
     2821 cggaatgagc gtgaccatga aggataagga ctttaatccg ctgattgggc gtaccggtgt
     2881 tgatgtgggt aaatccttct ccggtaagga ctggaaagtc acagcccgcg ccggccttgg
     2941 ctaccagttt gacctgtttg ccaacggtga aaccgtactg agtgatgcgt ccggtgagaa
     3001 acgtatcaaa ggtgaaaaag acggtcgtat tctcatgaat gttggtctca acgccgaaat
     3061 tcgcgataat cttcgctttg gtcttgagtt tgagaaatcg gcatttggta aatacaacgt
     3121 ggataacgcg atcaacgcca acttccgtta ctctttctga taacagcccg ggccgcgttt
     3181 gcggcccttc ttctaccgga gagaatatgt attaccctgt gacagactat atcgctcttg
     3241 ctctcattat tagctttctt tttctgacat tatttatctg cctgttatgc cttaaacatg
     3301 agcgaataaa aaaagaaact atcaggcaaa aaaaatgcac atattctgga gcatggctgg
     3361 aatgcaactg agttctcatg gttccgatac ggacagtata acgaaacggg tatttacatc
     3421 tcgatcgaga agaccattat cattacaatc accgtttctg gtgggtgctt taaaaaagaa
     3481 tacagcattg tctcccacat gctggtcact gacaccataa cagaggccac cctgtacgaa
     3541 aacgggctgt atacgcgcca catcagactc agtcgcccag tatctgacag caaatacccc
     3601 ttgccacccg gatctcagct cattaaaaat atgaccttac gactgcgcct gcaggatcag
     3661 caggaagaaa catccgtgac attatttcag ggaaaaatga gtaccgacgg aaataactat
     3721 tacatcatca aaggtaaagt aagctcagta cttctgttat taaaaatgct gcagattaat
     3781 cacgcctgat ctctgtcccg gtattttagc ccgaggtata ttctgaaata tcattcatca
     3841 tgaccggcgt ttatacaaac gccggtcatt cgctaacagc ttcaccgttc cttcccccat
     3901 ccaggttccg catgagcgtg ttgtaatgtt cataagcgta ttatcgtaag cttccgggga
     3961 actcatattg acgcgttttt caggcgcgcg tcatcggcgg cgcatacgaa cgatcgtggt
     4021 ttgataacgg ttgatttatt ttgttgcaca ggggcgatcg ctaggcaggt accggaggcg
     4081 ggctctgaag tactgagtag ggtgatcgcg tcaggccgcg aagtactgac tcggggatta
     4141 atagcaggtg atgagtaagc gtacagcgag ggccgtattg acggggatgt gttattcagc
     4201 tggcagtgct atgcgccacg gaagcagttc gctgacccgg ttgaccggcc agtctgctat
     4261 gacgccaagc acatggcgaa ggtagctttc tggatccacg tcattcagtt tgcacgtccc
     4321 gatcaggctg tacagtagcg ctccccgctc accaccatgg tcagagccga agaacaggaa
     4381 gtttttacga cccagactga ccgcccgcaa ggcattttca gcgatgttgt tgtcgatttc
     4441 cacccagcca tcgttcgcat agtacgtcag tgccggccac tggttaagtg cgtacgcgaa
     4501 cgccttcgcc aactctgagt gtcgcgacag ggtcttcatc ttttccgcaa ccagctttcc
     4561 agggatttca acagcggttt cgtttttcgc tgacaccatg gagggagtgg ttaaaagagc
     4621 aacgagaaag tggtaattat aacgccagcc ggatatggcg ggaaatggtg gcccggggat
     4681 ttacaggcag tgaaaccatc gtcagggatg cggttgccaa atggcgtaaa ggctggaacc
     4741 caccggttac tactgccgtc agacttcctt cagtgtcccg ggtaagccgc tggttgatgc
     4801 cctggagaat aaccagggat gaagaaaatt atgcttcccg atttattagt ctgatgtgtg
     4861 aaaaagaacc tgagctgaaa atagcgcagc aactggcact cgagttctac cgtattctga
     4921 aaacccaaaa taaatcacag ctaagcagtt ggttcactcg agtccacgaa agcggttcag
     4981 cagaatttcg gcgcgtggcg gcggggatgg aagctgatgc tgcagctata tgtgaggcaa
     5041 tcagtagtcg ctggagtaat ggtgttgtcg aaggtcatgt aaatcgcctg aagaagatgc
     5101 tgaaacgcca gatgtatggt cgagcaggat ttgaactact caggcagagg gtaatgagtc
     5161 cactggcatg aaaatctcca cccaggttgc ggaagagcca cttttcatcc gtcgttgaca
     5221 gttataccgc cagtcagaaa tataccgtcg tcagggcgtg gagctgagcc gtgccacact
     5281 gggtcgctgg accggtgccg ttgctgaact gctggagccg ctg
//
LOCUS       DAZPEB010000221         5273 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZPEB010000221 DAZPEB010000000
VERSION     DAZPEB010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5273
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.226"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..1324
                     /gene="ipaB"
                     /locus_tag="ACHURJ_003724"
     CDS             <1..1324
                     /gene="ipaB"
                     /locus_tag="ACHURJ_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HFR1282493.1"
                     /translation="INKLKNADSKIKDLENKINQIQTRLSELAPDSPENKKLRREEIQ
                     LTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLEKEIDSFSAFSNTASAEQLSTQQKS
                     LTGLASVTQLMATFIQLVGKNNEESLKNDLALFQSLQESRKTEMERKSDEYAAEVRKA
                     EELNRVMGCVGKILGALLTIVSVVAAAFSGGASLALAAVGLALMVTDAIVQAATGNSF
                     MEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVDSKKAKMIGSILGAIAGALVLVAAV
                     VLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFLKNFSSQLDDLITNAVARLNKFLGA
                     AGDEVISKQIISTHLNQAVLLGESVNSATQAGGNVASAVFQNNASKNLADLTLSKYQV
                     EQLSKYIGDAIEKFGQLQEVIAELLASMSNSQANRTDVAKAILQQTTA"
     gene            1287..2435
                     /gene="ipaC"
                     /locus_tag="ACHURJ_003725"
     CDS             1287..2435
                     /gene="ipaC"
                     /locus_tag="ACHURJ_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HFR1282494.1"
                     /translation="MLQKQFCNKLLLDTNKENVMEIQNTKSAPILYTDISTKQTQSSS
                     ETQKSQNYQQLAAHIPLNVGKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKD
                     LSEMSHTITPENTLDISSLSSNAVSLIISVAVLLSALRTAETRLGSQLSLIAFDATKS
                     AAENIVRQGLAALSSSITGAVTQVGITGIGAKKTHSGISEQKGALRKNLATAQSLEKE
                     LAGSKLGLNKQIDTNITSPQTNSSTKILGKNKLAPDNISLSTEHKTSLSSPDISLQDK
                     IDTQRRAYELNTLSAQQKQNIGRATMETSAVAGNISTSGGRYTSALEEEEQLISQASS
                     KQAEEASQVSKEASQATNQLIQKLLNIIDNINQSRNSTASQIAGNIRA"
     gene            2486..3484
                     /gene="ipaD"
                     /locus_tag="ACHURJ_003726"
     CDS             2486..3484
                     /gene="ipaD"
                     /locus_tag="ACHURJ_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HFR1282495.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKDLSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSKK
                     EYPINKDARELLHSAPKEAELDGYEMISHRELWDKIAKSINNINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVKSLKDELTKLKEKYKDKPLYPANNTV
                     SKEQANKWLTELGGTIGKVSEKNGGYVVNINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            3493..>5273
                     /gene="ipaA"
                     /locus_tag="ACHURJ_003727"
     CDS             3493..>5273
                     /gene="ipaA"
                     /locus_tag="ACHURJ_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HFR1282496.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNDLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKVFNHTPDN
                     SDGIGSDLLTTSSQERSTNNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANTINYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSKIDDTSAELLT"
BASE COUNT         1968 a         1067 c          886 g         1352 t
ORIGIN      
        1 aattaataaa ctaaaaaacg cagattctaa aataaaagac ctagaaaata aaattaacca
       61 aattcaaaca agattatccg aactcgcccc agattcacca gaaaataaaa aattaagacg
      121 ggaagaaata caactcacta tcaaaaaaga cgcagcagtt aaagacagga cattgattga
      181 gcagaaaact ctgtcaattc atagcaaact tacagataaa tcaatgcaac tcgaaaaaga
      241 aatagactct ttttctgcat tttcaaacac agcatctgct gaacagctat caacccagca
      301 gaaatcatta accggacttg ccagtgttac tcaattgatg gcaaccttta ttcaactagt
      361 tggaaaaaat aatgaagaat ctttaaaaaa tgatctggct ctattccagt ctctccaaga
      421 atcaagaaaa actgaaatgg agagaaaatc tgatgagtat gctgctgaag tacgtaaagc
      481 agaagaactc aacagagtaa tgggttgtgt tgggaaaata cttggggcac ttttaactat
      541 cgttagtgtt gttgcagcag ctttttctgg aggagcatct ctagcactgg cagctgttgg
      601 tttagctctt atggttacgg atgctatagt acaagcagcg accggcaatt ccttcatgga
      661 acaagccctg aatccgatca tgaaagcagt cattgaaccc ttaatcaaac tcctttcaga
      721 tgcatttaca aaaatgctcg aaggcttggg cgtcgactcg aaaaaagcca aaatgattgg
      781 ctctattctg ggggcaatcg caggcgctct tgtcttggtt gcagcagtcg ttctcgtagc
      841 cactgttggt aaacaggcag cagcaaaact tgcagagaat attggcaaaa taataggtaa
      901 aaccctcaca gaccttatac caaagtttct caagaatttt tcttctcaac tggacgattt
      961 aatcactaat gctgttgcca gattaaataa atttcttggt gcagcgggtg atgaagtaat
     1021 atccaaacaa attatttcca cccatttaaa ccaagcagtt ttattaggag aaagtgttaa
     1081 ctctgccaca caagcgggag gaaatgtcgc ttctgctgtt ttccaaaaca acgcgtcgaa
     1141 aaatctagca gacctgacat tatcgaaata tcaagttgaa caactgtcaa aatacatcgg
     1201 tgacgcaata gaaaaattcg gccaattgca ggaagtaatt gcagagctgt tagcctcaat
     1261 gtccaactct caggctaata gaactgatgt tgcaaaagca attttgcaac aaactactgc
     1321 ttgatacaaa taaggagaat gttatggaaa ttcaaaacac aaaatcagcc ccgattttat
     1381 atacagatat atctacaaaa caaactcaaa gttcttccga aacacaaaaa tcacaaaatt
     1441 atcagcagtt agcagcacat attccactta atgtcggtaa aaatcccgta ttaacaacta
     1501 cattaaatga tgatcaactt ttaaagttat cagagcaggt tcagcatgat tcagaaatca
     1561 ttgctcgcct tactgacaaa aagatgaaag atctttcaga aatgagtcac acaattaccc
     1621 cagagaacac cctggatatt tccagtcttt cttctaatgc tgtttcttta attattagtg
     1681 tagccgttct actttctgct ctccgcactg cagaaactag attgggctct caattgtcat
     1741 tgattgcgtt cgatgctaca aaatcagctg cagagaacat tgttcggcaa ggcctagcag
     1801 ccctatcatc aagcattact ggagcggtca cacaagtagg tataacgggt atcggtgcca
     1861 aaaaaacgca ttcagggatt agcgaacaaa aaggagcctt aagaaagaac cttgccactg
     1921 ctcaatctct tgaaaaagag cttgcaggtt ctaaattagg gttaaataaa caaatagata
     1981 caaatatcac ctcaccacaa actaactcta gcacaaaaat tttaggtaaa aataaactgg
     2041 caccagataa tatatccctg tcaactgaac ataaaacttc tcttagttct cccgatattt
     2101 ctttgcagga taaaattgac acccagagaa gagcttacga gctcaatacc ctttctgctc
     2161 agcaaaaaca aaacattggc cgtgcaacaa tggagacatc agccgttgct ggtaatatat
     2221 ccacatcagg agggcgttat acatctgctc ttgaagaaga agaacaacta atcagtcagg
     2281 ccagcagtaa acaagcagag gaagcatccc aagtatctaa agaagcatcc caagcgacaa
     2341 atcaattaat acaaaaatta ttgaatataa ttgacaacat caaccaatca aggaattcta
     2401 cagccagtca gattgctggt aacattcgag cttaatatat ccaagagcca taataatata
     2461 tggctcttcc tataaggaaa taaccatgaa tataacaact ctgactaata gtatttccac
     2521 ctcatcattc agcccaaaca ataccaacgg ttcatcaacc gaaacagtta attctgatat
     2581 aaaaacaacg accagttctc atcctgtaag ttcccttact atgctcaacg acacccttca
     2641 taatatcaga acaacaaatc aggcattaaa gaaagatctc tcacaaaaaa cgttgactaa
     2701 aacatcgcta gaagaaatag cattacattc atctcagatt agcatggatg taaacaaatc
     2761 cgctcaacta ttggatattc tttccaagaa agaatatcca attaataaag acgcaagaga
     2821 attattacat tcagccccga aagaagccga gcttgatgga tatgaaatga tatctcacag
     2881 agaactgtgg gataaaattg caaagtcaat caataatatt aatgaacagt atctgaaagt
     2941 atatgaacat gccgttagtt catatactca aatgtatcaa gattttagcg ctgttctttc
     3001 cagtcttgcc ggctggatct ctcccggagg taacgacgga aactccgtga aattacaagt
     3061 caaatcgctt aaagacgaat tgacaaagct caaggaaaaa tataaagata aaccgctata
     3121 tccagcaaat aatactgtta gtaaggaaca agcaaataaa tggcttacag aattaggtgg
     3181 aacaatcggc aaggtatctg aaaaaaacgg gggatatgtt gtcaatataa acatgacccc
     3241 aatagacaat atgttaaaaa gcttagataa tctaggtgga aatggcgagg ttgtgctaga
     3301 taatgcaaaa tatcaggcat ggaatgccgg attctctgcc gaagatgaaa caatgaaaaa
     3361 taatcttcaa actttagttc aaaaatacag taatgccaat agtatttttg ataatttagt
     3421 aaaggttttg agtagtacaa taagctcatg tacagataca gataaacttt ttctccattt
     3481 ctgaggtgcg ccatgcataa tgtaaataat actcaagcgc caacattctt atataaggca
     3541 acttcaccat catcaacaga atacagcgag ttaaaaagca aaatatccga tatccatagt
     3601 tcgcaaactt ctctaaaaac accagcatca gtgtctgaaa aagaaaactt tgcaacgtct
     3661 tttaatcaga aatgtcttga ttttttattt tcttcctcag ggaaagaaga tgtgttaaga
     3721 agcatttatt ccaactcaat gaatgcgtat gccaaaagcg agattctcga attttcaaat
     3781 gttttgtact ccttagtaca tcaaaatgat cttaattttg aaaacgaaaa gggacttcaa
     3841 aaaattgtcg cacagtattc ggaactaatt ataaaagata aattatccca agattctgcc
     3901 tttggaccat ggtcggcaaa gaataagaaa ctccatcaat tacgacaaaa cattgagcac
     3961 agacttgcac tattagcaca acaacacaca tctggtgaag ctttatcatt gggacaaaaa
     4021 ctcctcaata ctgaagtatc atcatttatc aagaataata ttcttgctga attaaagtta
     4081 agtaatgaaa ctgtttcatc tctcaaacta gatgatttag ttgacgcaca ggcaaaactt
     4141 gcctttgata gtttgcgcaa tcaacgtaaa aatactattg atagtaaagg atttggtata
     4201 ggtaaactgt caagagactt aaatacagta gccgtgtttc ctgagctgtt gagaaaagtc
     4261 cttaatgata ttttagaaga tataaaagat tcgcatccta tccaagatgg cctccctaca
     4321 cctcccgaag atatgccaga tggcggacca acccccggag ccaatgagaa aacatcccaa
     4381 cctgtaattc actatcatat aaataatgat aatagaactt acgataatag agtttttgac
     4441 aacagagtat atgacaatag ctatcacgag aacccagaaa atgatgcaca gtctcctact
     4501 tctcagacaa acgatctatt atcccgtaac ggaaactcat tactaaatcc acaaagagca
     4561 ctagttcaaa aagtaacttc cgttctacca cactctatat cagatactgt ccagacattt
     4621 gcaaataatt cagctttaga aaaggttttc aaccatactc cagataattc ggatggaata
     4681 ggttcagacc tgttaactac gagtagtcaa gaaagatcta caaataactc tctttctcgg
     4741 ggacacaggc ctctgaacat acagaactct tcaaccaccc cccctctcca cccggaagga
     4801 gtgacaagca gtaatgataa ctcatcagat acaactaaaa gtagcgcttc tctttctcat
     4861 agagtagctt cgcaaatcaa taaattcaac tcaaacactg attcaaaagt acttcagact
     4921 gattttttat caagaaatgg agacacatat ttaacacggg aaacgatatt tgaagcttca
     4981 aaaaaagtaa caaactccct aagtaatctt atatctctca ttggaactaa atcaggaaca
     5041 caagaacgag agttacagga aaaatcaaag gacattacaa aatccacaac agaacacaga
     5101 ataaacaaca aattaaaagt tacagatgca aatacaataa actacgtaac agaaaccaac
     5161 gcagatacaa ttgataaaaa tcatgcgatc tatgaaaagg caaaagaagt atctagcgcc
     5221 ctcagcaagg tattgtcaaa aattgacgat acctctgcag aattacttac aga
//
LOCUS       DAZPEB010000222         5208 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZPEB010000222 DAZPEB010000000
VERSION     DAZPEB010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5208
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.227"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..117
                     /locus_tag="ACHURJ_003728"
     CDS             <1..117
                     /locus_tag="ACHURJ_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3829 domain-containing protein"
                     /protein_id="HFR1282497.1"
                     /translation="DWDKEQLQDANSSWMVEDSFPRALREYNEMVDDYNSLR"
     gene            complement(167..1915)
                     /locus_tag="ACHURJ_003729"
     CDS             complement(167..1915)
                     /locus_tag="ACHURJ_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HFR1282498.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARISASGSAGYQLEIFERRSFFQLLQKHDNDYRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERACCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EQSGMFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPDIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDNDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLNCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLEDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASILCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(1915..2985)
                     /locus_tag="ACHURJ_003730"
     CDS             complement(1915..2985)
                     /locus_tag="ACHURJ_003730"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFR1282499.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGSVAPHALTEKQRQRPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKSCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQMD"
     gene            complement(2975..4426)
                     /locus_tag="ACHURJ_003731"
     CDS             complement(2975..4426)
                     /locus_tag="ACHURJ_003731"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HFR1282500.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFTGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEMQSGSVMSHLMASVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            complement(4437..4883)
                     /locus_tag="ACHURJ_003732"
     CDS             complement(4437..4883)
                     /locus_tag="ACHURJ_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFR1282501.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHKEFIHQLQQGDTDQVLALLNQTLSS"
BASE COUNT         1318 a         1341 c         1316 g         1233 t
ORIGIN      
        1 gactgggata aagagcaact tcaggacgca aactcaagct ggatggtcga agactctttc
       61 ccgagagcat tacgcgagta caacgaaatg gttgatgact ataatagcct gcgttaaacg
      121 tttttgatga taaatgcagt gtcggatgcg gcgcatctga cacagttcac cccttgtgaa
      181 gtaactccat aaatgcctca tatcctgtta acccggcaat aacctctgcc ggatgctgac
      241 acaatatgct tgccagatag ctaaatattt ttagctcatg acgcgcatcg gcggcggcgc
      301 aggcgatcaa gacatggtta atgacttcgt tattcacctc aactggttgg gcgagggtaa
      361 taaaaaatcc acgaaaacgt cgctcctgtt cgctccagca atgtgggatg gcgaggcgat
      421 taacaatcag gttttcacct tcgccttcgc gggcgatgat gcgttgtgct tcatcggcgg
      481 taatatggtg ttgtgcgact aattgcgcac aaatttgccg ggtaatatgt tgccagcttt
      541 caccgcgtac attcgaataa tgaaaacttc cgggggcaga gaaaaaacgc tccggttgct
      601 ggcgaataaa tgccgtcgcc agaaaatgtt ttatttgttc gataccggca gccgtaatga
      661 tattttttac ggtgatgtaa ttatttaccg cgtcttccag taaataatgg ctattgttaa
      721 tgatcaataa cggctcaatc tcttcgcgaa tggcaacaag ttcgcttaag ctacgggcaa
      781 taattacccg acaatttaaa acatcgcgct caatggcgag ctgattaata gtggcaatcg
      841 cgttctggtc cgagagcaaa atgatcggct ggcgttcgtt ttgatgccgc tccagcgcac
      901 aggcaaaata caaaccgatc agatcgttgt cgaaaagggg aatatcgagc tgttcacgca
      961 gtagcgtaat aaagtgcaga ctcatatcaa aggccgcagg ccaggctgct ttcaggttat
     1021 tcatgctact ttggcggtgc tcagcaatcc acaccggtgc ggcaaggcaa cgtaataaat
     1081 gcccggtaat attctcaacc aactgcgtat caataatatt caatgacgcg atgtctggca
     1141 ccctttgcag caaccccagc acatgatcgg ctgaaattac ctgcgcttgc tgatgctgtt
     1201 tctctatcaa acctgtcagg gttcttacag cgttatcacc aagaaacatg ccgctttgtt
     1261 caacgatttg ttttatctcg tcaccgggat attgcggcca ctcatcggtc agttgattac
     1321 gcagggcgta aatcgccaga atcaggctgg agagataatc accctgcatg agcggccagc
     1381 ggtgttggtt gtcgcaggcc gtggataaat gctgaaggtt gtctcgtgtt acgcccgcca
     1441 gcggaattaa aaacggatct ttgcgcagca agttcgccag caagataacg cgcttctctt
     1501 ctgtctcatc aatgaaatgg cccaaaccgg ggcggctggc caggcaacaa gcgcgttcat
     1561 aacgctgttt taaccggggc agacgctctg cgacccacgt ttctggcaaa ttaagcgccg
     1621 aggcgagttg cgcacgggga gtaaaggtat tcagtaataa cagtgccagc agccgatagt
     1681 cgttatcgtg cttttgcagc aactgaaaaa agctgcggcg ctcgaagatt tccagctgat
     1741 agcccgcact accactggcg gaaatgcggg ctttgccgtt aagggtaaag ttgagatagt
     1801 caatatcacg caggatggtc ctgcctgaaa cgccagtctg ttgcgccagc tcaccaggcg
     1861 tgcgcggctg ttgctccagc agatcgacaa tctttaactg gcgttcgttt agcattaatc
     1921 catctgacgg aactggctaa acgcgttgac tttctccgcg gttaaggtgg tcagaaaatc
     1981 ccgtatcaac gtgagcagag catcgtaatc ggcttttgaa atcataccgc tgttagcgtg
     2041 cagatagcgg gttggcaggc acagcgcaac aaccggacgc ccgccgccca tcacgttgta
     2101 gcgcccgcca tccgtcgcac cggttttcat ggtggaaaat tgcagcggta aatcgttatg
     2161 tgcggcacag ctttttaacg ctgctaccag tttctggttg gggaagtagc gcttgtcaaa
     2221 cagcatcagc cccggcccct ggcccagttt cagcgggtat ttaatgttat caatgcccgg
     2281 aacatcgcct gcgacagcgg tatccagcac gatcactaca tccggtttaa tgtgttccgc
     2341 cgaggtttgt gccccgcgta gcccaacttc ttcttccacg ctgccgacgc catacagcgt
     2401 aatttcggga ttattcaccg tctgcaatag ttcagccatc attgcgcagc cgatgcggtt
     2461 atccagcgcc ttgccgacca ctttatcttc gccccagcag gcaaaattgg cttccgggct
     2521 aataaaatcg cccatcgcca caccgcgttt ttccacctca tcgcgactgt ttgcgccaat
     2581 atcaatgaac atctcatcaa atgacagcgg ccgttgcctt tgcttttccg ttaacgcatg
     2641 aggcgcgacg gaaccaatca caccagggat tttcactccc ttgtgtgtgc gtatggttac
     2701 ccggtggttg agcatcgact gattccacca gccaccaatg gtggtgaaac gcagaaaacc
     2761 gctctcgtcg atgtgggtga ccataaagcc gacttcatcc atatgcccga caacggcaac
     2821 ttttggacct ttattcccct tacgggcaac aaagctgccc agaccatcaa aggtgatctc
     2881 attaacgcaa ggttccagcg tgtttatcag aatgtcgcga acttcctgtt catcgccgct
     2941 gacggcgctg gcttcgcaca actgttgcag taactcaatg ttcatggaca agctcctgtt
     3001 gcgcggcttt cgctttgcgg cgtaaccaca atcctttcag gacgataatg aatgaaatgt
     3061 tcagcgcgag gccaatcgcc agcaccagat aaaacgaacc caccggcgac atcaggccga
     3121 tcagcgggtc aaagataccc aaaccaggtg ccagacgttt gattccgaaa gcgatcacca
     3181 gcacgccggt aatgccaccc gccagcgtat tggcggtaat catcggcaac ggagctgcca
     3241 gagcgtaagg tatagccggt tcggttgcga ctgtggcacc gaccacaatg gcactactgg
     3301 cagcctcttt ttcctgtcgg gtaaacagtt tgggcgcaat gaacgtcgca agacccgccg
     3361 caacgggcgg catcaacgcg acaacgccga cgatggcgta ccagtcataa atgtgttttt
     3421 ccagcagtga gaagcagaag aaccaggcgg ttttattgat ggggccgccc atatcgaacg
     3481 ccagcatcgc accgaccaga aacgctgctg caaacttcat tgatggagga atggtattca
     3541 ggaagtggag cagcccgccc atgaggtcgg acatcaccgg cccgataacg tagtaagtga
     3601 gcacgccgaa aaccagcaga gtaacgaacg ggatcagcat tgaaccgagc agcggttgta
     3661 atgctttgcc aaggcgcact ttgcggaacc agaagacgaa atagccaatc gccagcccca
     3721 gcaccaccgc gccgaggaat cccgcacccg actgggtgcc gagcagcgct ttgtcgttcg
     3781 ccagatagca aaccagaaac gccggagcga aagccggttt atcggcaatc gacgaggcaa
     3841 tataagcgcc catgatgggg atcataaagg tgaagccaag gtaaccgatg gattcaacta
     3901 cccaggtaaa cgacggcgcg cctttcgaca tatcggtata cggcaaaccg aactgcacca
     3961 gcatgttggc aagcgccacc aggataccac cgccgatgac aaacggcagc gccgcagaga
     4021 cgctcgccat caggtggctc atcacgctgc cgctttgcat ttcctgtttg ccaagcttca
     4081 cgccactatc tgcggcaaaa agctgcgagt ttgtcggtaa ttcgctgaaa atctggtcga
     4141 tatttttcaa cgcctgggag atggcaatct cataaacttt cttcccggta aaccgcgcgc
     4201 gatcatcacc gctcaggcca cgcccggtag cgagaatgac gtaatcggca gcggcaatct
     4261 cttcgctgga taagcggttt tcaacgccac tggacccttg agtttccact tttatggtat
     4321 ggccgagaga acgggctttc tgttccaggg cttccgccac catgtaggtg tgagcgatcc
     4381 cggcggggca gttggtgatc gcgacaatac gtaaggatga ctccatcgcc acttccttat
     4441 gagctgaggg tttgatttaa caaggcaagc acttggtcgg tatcgccctg ttgcagttga
     4501 tgaataaatt ccttgtgaat aattttgcga cacagtgtgc cgatgatttt gacctgatcc
     4561 tcttcgccgc tttgcggcac gccgaggcag atccagcaat tgacgtcttc gccatcgctg
     4621 gctttccagt caatagcctg cgctttgcgg gcgaataata cgaacggttg tttaacgcag
     4681 gcgcttttac cgtgcggcac ggcgacaccg gaaccaaatc cagtagagtg cattttttcg
     4741 cgcagcagca gggtttgcag gaactggtgt gagtcggtga taaaaccgtt ttgtaacgct
     4801 attgtcgcca actgcttcag aacggaataa gcgccattgc cctgaatatt caggtcaata
     4861 cagcttgcag taagagctgc cattaatcgg actccataaa atatatttta atcaattgta
     4921 atggtggagc gattaagtga tgagaggcga tatgtcggta aacaatgaca atttgagaca
     4981 gagtgaaagg tcagatttgc ggagtaatgc acataatggt tatttaaata aaccacatga
     5041 atcattaatg ggttattcat tatttttgtg atttactcac aatgtatcag ggaaatataa
     5101 cttaccggga gatgtaatat gtttaatttt tcaaatcgaa atttaaaata ttgtgccgga
     5161 ggcatctccg gcacattggg caattacggc aggtaaaaca cttcttgc
//
LOCUS       DAZPEB010000223         5020 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZPEB010000223 DAZPEB010000000
VERSION     DAZPEB010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5020
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.228"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            142..2139
                     /locus_tag="ACHURJ_003733"
     CDS             142..2139
                     /locus_tag="ACHURJ_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HFR1282502.1"
                     /translation="MSENDTIPKKSTSQINKAVFFTSALLIFLLVAFAAVFPDVADKN
                     FKLLQQQIFTNASWFYILAVALILLSVTFLGLSRYGDIKLGPDHAQPDFSYHSWFAML
                     FSAGMGIGLMFFGVAEPVMHYLSPPVGTPETVAAAKEAMRLTFFHWGLHAWAIYAIVA
                     LILAFFSYRHGLPLTLRSALYPIIGDRIYGPVGHAVDIFAVIGTVFGVATSLGYGVLQ
                     VNAGLNHLFGVPINETVQVILIVVITGLATISVVSGLDKGIRILSELNLGLALLLLAL
                     VLCLGPTVLLLKSFVENTGGYLSELVSKTFNLYAYEPKSSNWLGGWTLLYWGWWLSWS
                     PFVGMFIARVSRGRTIREFVTGVLFVPAGFTLMWMTVFGNSAIYLIMNQGATDLANTV
                     QQDVALALFNFLEHFPFSSVLSFIAMAMVIVFFVTSADSGAMVVDTLASGGVANTPVW
                     QRIFWASLMGIVAIALLLAGGLSALQTVTIASALPFSVILLISIYGLLKALRRDLTKR
                     ESLSMATIAPTAARNPIPWQRRLRNIAYLPKRSLVKRFMDDVIQPAMTLVQEELNKQG
                     TISHISDAVDDRIRLEVDLGNELNFIYEVRLRGYISPTFALAAMDNDEQQTEQHRYYR
                     AEVYLKEGGQNYDVMGWNQEQLINDILDQYEKHLHFLHLVR"
     gene            2202..3572
                     /locus_tag="ACHURJ_003734"
     CDS             2202..3572
                     /locus_tag="ACHURJ_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001409274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HFR1282503.1"
                     /translation="MISRWKWMLKQTIKKLWFRATLFAIVAIITALLSILFKSMIPES
                     VSVKVGAEAVDNILNILASSMLAVTTFSLSIMVTAYGSATTNVTPRATRLVVEDVTTQ
                     NVLATFIGSFLFSLVGIIALNMGAYGERGRVILFIVTLVVIALILITLLRWIQHLTSL
                     GRVGETTAQVEQAAIETFIARARNPCLGGYPWLENNEQPKGTVAVYPKKIGYVEYIDM
                     VKLSKLLTNDPRHVYLVAQPGSFIHPSMPVLYLSQGQESSINTDLLETIIVSDARSFA
                     QDPRFCLSVMAEIACRALSPAVNDPGTAIDVIGRGVRILSTYAQNKSDEIEVKYPSVH
                     VAPLQNNDLLEDFFSPVARDGASMREIQIRVLKGLSMLSKGWPEIFAEAAHTLAFETL
                     EHATRADHIDSDRYLIKSTYYNLFSGEYSNKKNSCRASNSVVGKSRAKHSRENGRMLC
                     AKSKRC"
     gene            3724..3885
                     /locus_tag="ACHURJ_003735"
     CDS             3724..3885
                     /locus_tag="ACHURJ_003735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282504.1"
                     /translation="MTGENRGRGRPRKTESTYADTRNDLIRSGLELLTQNGFLATGVD
                     AIVSTTDEK"
     gene            complement(3929..4711)
                     /gene="istB"
                     /locus_tag="ACHURJ_003736"
                     /pseudo
     CDS             complement(3929..4711)
                     /gene="istB"
                     /locus_tag="ACHURJ_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
     gene            complement(4708..>5020)
                     /locus_tag="ACHURJ_003737"
     CDS             complement(4708..>5020)
                     /locus_tag="ACHURJ_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFR1282505.1"
                     /translation="QYTRNSFYIPLMTRLRPMGITVDVETANRHGLRWLHDVANQRKH
                     ETIQARPCDRWLEEQQSMLALPPEKKEYDVHPGENLVNFDKHPLHHPLSIYDSFCRGV
                     A"
BASE COUNT         1182 a         1174 c         1282 g         1382 t
ORIGIN      
        1 tggtagtgtc cgctattgcc agtacacctc aacattccac catgcattcc gattaacgcc
       61 gcatagccag ttgaactttg ctactttgtg agaggtagta ccttctatcc agtgcgaatt
      121 taattaatgg aataaatgat tatgagtgaa aatgatacaa tcccaaagaa gtctacaagt
      181 cagattaaca aagcggtatt ctttacatct gctttgctaa ttttccttct tgtcgccttt
      241 gccgccgtat tcccggatgt cgccgacaaa aattttaaac tacttcagca acaaatcttc
      301 acgaatgcca gctggttcta catccttgct gtggccctga ttttactgag tgtcacgttc
      361 cttggactct cacgctacgg tgatatcaag ctgggcccgg accatgcgca gcctgatttc
      421 agctaccact cctggtttgc gatgcttttt tcggcaggga tggggatcgg cctgatgttc
      481 tttggcgttg ccgaacctgt aatgcattat ctttcgccac ccgttggcac tccagaaacc
      541 gttgcggcag ctaaggaagc aatgcgtctg acctttttcc actggggact gcacgcatgg
      601 gcaatttatg ccattgtggc gctgattctg gcgttcttca gttaccgtca cggtctgcct
      661 ttaactctgc gctccgcact ctatcccatt attggcgatc gcatatacgg acctgtagga
      721 catgcggttg atattttcgc tgttataggc acggtctttg gcgttgcgac atcactgggt
      781 tacggtgttt tgcaggtgaa tgccggtttg aaccatcttt tcggggtgcc catcaatgaa
      841 acggtgcagg taattctgat cgtggtcatc acggggttag cgacgatttc agtggtgtcc
      901 ggtctggata agggaatacg tatcctgtct gaactcaatc tgggtctggc tttgttgctc
      961 ctggcgctgg tcctgtgtct gggaccaacc gtgcttctgc tgaagtcatt tgtggaaaat
     1021 acgggcggtt atctttcgga actggtgagt aaaacgttca acctttacgc gtatgagccc
     1081 aagtcgagca actggctggg gggctggaca ttactgtact ggggatggtg gctttcatgg
     1141 tcgccgtttg tggggatgtt catcgcacgg gtctcccgcg ggcgaaccat tcgcgagttt
     1201 gtcaccggcg tgctgtttgt tcccgcgggt tttacgctaa tgtggatgac ggtgtttggt
     1261 aacagcgcga tctatctcat tatgaaccag ggggccacag acctcgccaa tactgttcag
     1321 caggatgtgg cgctggccct gtttaatttc ctggagcatt tcccgttctc ttctgtgctg
     1381 tcattcattg caatggcgat ggtcatcgtc ttctttgtaa cgtctgctga ttcgggggca
     1441 atggttgtgg atactctggc atcaggtgga gtggcaaaca cacccgtctg gcagcgaata
     1501 ttctgggcct cgctcatggg cattgttgca attgcgcttc tccttgccgg agggctaagt
     1561 gcgctgcaaa cggtgacaat agcgagtgca ttgcccttct cagtgatctt actaatatct
     1621 atatacggac ttttaaaagc tttgcgccgg gatttgacca agcgtgaaag cctgagcatg
     1681 gcgacaattg ctcctacggc tgcacgtaac ccaattcctt ggcagagaag gttacgcaat
     1741 atcgcgtatc tgccgaagcg atctcttgtg aaacgtttta tggacgacgt tatccagccc
     1801 gccatgacgc tggttcagga ggaactgaac aagcagggga cgataagcca cattagtgat
     1861 gcagtcgacg atcgtattcg tcttgaagtc gatttgggca acgagctgaa ttttatatat
     1921 gaagtgaggc ttcgcgggta tatctcaccg accttcgcgc tcgccgcaat ggataatgat
     1981 gagcagcaga ctgaacaaca tcgatattat cgcgctgagg tttatctcaa agaaggcggt
     2041 caaaattatg atgtgatggg ctggaaccag gaacagctga ttaatgacat actggaccag
     2101 tacgaaaaac acctgcactt cctgcacctg gttcgttaat agcaacatgc cgtcctgggg
     2161 gcggcaatta ttatcccggc cgcaatatga gggaatgcag aatgatttca cgctggaaat
     2221 ggatgctgaa gcagacaatt aaaaaactat ggttcagggc aacgttattc gcaattgtcg
     2281 cgataataac ggccctttta tcaattcttt ttaaatcaat gatacctgag tcggtttccg
     2341 tgaaggttgg tgcggaagca gtcgataaca ttctgaacat actggcatcg agtatgctgg
     2401 cagtgaccac attttcgctg agtatcatgg tcacagccta cggttcagcc actactaatg
     2461 tgactcccag agctacgcgt ttagttgttg aagacgtcac cacacaaaat gtactggcca
     2521 ccttcatcgg ttcttttctc ttcagtctgg tagggattat tgccctcaat atgggagctt
     2581 atggagaaag ggggagagtc attttattca ttgtcacact ggttgtcatt gccttaatcc
     2641 tcatcacatt gcttcgctgg atacagcatt tgacctctct ggggagggtt ggtgagacaa
     2701 cggcacaagt agaacaggcg gccatcgaaa catttattgc gagagcaaga aatccctgtc
     2761 tcggcggata tccatggctt gagaacaatg aacagccgaa aggaacggtt gcagtttatc
     2821 cgaagaagat tggctatgtt gaatatattg atatggtgaa acttagcaag ctgctgacca
     2881 atgatccccg tcatgtatac ctcgtggcgc agccaggcag tttcatacat ccgtccatgc
     2941 cagttttgta cctgagtcag ggccaggagt cgtcaatcaa caccgattta cttgagacga
     3001 ttattgtctc ggatgcacgt tcatttgctc aggatcctcg attttgtctt agcgtcatgg
     3061 ccgaaatagc ctgccgggcc ctttcccctg cagtgaacga tcctggaacc gccattgatg
     3121 tcattggcag aggtgttcgt atactttcca cttacgcgca gaataaatct gatgaaatag
     3181 aagtgaaata tccttcagta catgttgcac cacttcagaa taacgatcta ctggaagact
     3241 ttttctcacc tgtcgcgcgc gatggtgcca gtatgaggga gattcagata agagtcctta
     3301 aaggactgtc gatgctgagt aaaggttggc ctgagatatt tgctgaagcc gcacataccc
     3361 tggcatttga aacattagag catgcaactc gtgctgacca tatagattct gatagatatc
     3421 taataaaatc aacttattat aatttattta gtggcgaata ttctaataaa aaaaatagct
     3481 gcagagcaag caattcggtt gttggcaagt caagggcaaa gcattcgcgt gagaatgggc
     3541 gtatgctttg tgccaaaagc aaacgttgct aattctgcac tgttcagcga tggcgcagta
     3601 gcgacaggca ctgccactgt ccagtcggca aaagcgaaca ctttgccact gcggggataa
     3661 actgattgaa gctctcgcgc aaaatcactt attcttttga caataaaatg taggagatgg
     3721 gtcatgacag gcgaaaatcg tggccgggga aggccgcgaa agacagaaag tacctatgca
     3781 gatactcgta atgatctgat ccgcagtggc cttgaactgc tgacgcagaa tggttttctt
     3841 gcgacgggag tcgatgccat tgtgtcaacg acggatgaaa agtgatccac ttatatctcc
     3901 accaacggcc caatattgat ccaccgtttt actcaggatt agcttcagct ataaccccgg
     3961 cctttcgttt ctgtctgagt cgatcgcttt ctcctttgat ttgaacgaca tgtgagtggt
     4021 gtaagatacg gtccagcatc gctgaggtca gtgctgcatc accggcgaac gtttgatccc
     4081 actgcccgaa cggcagattg gatgtcagga tcattgcgct cttttcgtaa cgtttagcga
     4141 tgacctagaa gaacagtttt gcttcttcct gactgaacgg cagatagcct atttcatcaa
     4201 tgatgagcag gcggggggcc attactccac gctgaagcgt cgttttataa cggccctgac
     4261 gttgtgccgt agataactga agtaacagat ctgctgctgt tgtgaagcga accttgatac
     4321 ctgcacggac tgcttcatag cccatcgcta ttgccagatg ggttttcccc acacctgatg
     4381 gccccagtaa tacgatattt tcattacgtt ctatgaagct gagtgagcgt aacgactgga
     4441 gttgcttctg cggtgctccg gtggcgaatg tgaagtcata ctcttcgaac gttttcaccg
     4501 ccgggaaggc tgccattcgg gtatacatcg cctgtttacg ttgatgacgt gccagttttt
     4561 cttcatgaag cagatgctcc aggaagtcca tataactcca ttcctggtct actgcctgtt
     4621 gtgacagcgc aggcgctgcg cttataaggc tttccagttg caactgcccg gcgagcgcca
     4681 tcagtcgttg atgttgcagt tccatcatca cgccactcct ctgcagaatg agtcgtagat
     4741 ggagagtgga tgatgcaggg ggtgtttatc gaagttcacc agattttcac caggatgcac
     4801 gtcatactct tttttctccg gaggcagtgc cagcatggac tgctgctctt cgagccagcg
     4861 atcgcaggga cgggcctgga ttgtttcatg ctttcgttgg ttagcgacat cgtgcagcca
     4921 gcgcagaccg tggcggttgg ctgtttcaac atcgacagtg atccccatcg ggcgcaggcg
     4981 agtcattagt gggatgtaaa aactgttacg ggtgtactgc
//
LOCUS       DAZPEB010000224         4965 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZPEB010000224 DAZPEB010000000
VERSION     DAZPEB010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4965
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.229"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..218
                     /locus_tag="ACHURJ_003738"
     CDS             <1..218
                     /locus_tag="ACHURJ_003738"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282506.1"
                     /translation="DIYNLWRKTRIKLIKGRYFNMLQIMLFLIHTRYKYQKHKPDKYL
                     INIQQSLNYSIYSSTTKRKKKNNNQEL"
     gene            392..1366
                     /gene="hemB"
                     /locus_tag="ACHURJ_003739"
     CDS             392..1366
                     /gene="hemB"
                     /locus_tag="ACHURJ_003739"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFR1282507.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1472..2323)
                     /gene="tauD"
                     /locus_tag="ACHURJ_003740"
     CDS             complement(1472..2323)
                     /gene="tauD"
                     /locus_tag="ACHURJ_003740"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFR1282508.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGITAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNCVTQHYANADYLPQRRIMHRVTILGD
                     KPFYRAG"
     gene            complement(2338..3147)
                     /gene="tauC"
                     /locus_tag="ACHURJ_003741"
     CDS             complement(2338..3147)
                     /gene="tauC"
                     /locus_tag="ACHURJ_003741"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFR1282509.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVSPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTP"
     gene            complement(3144..3911)
                     /gene="tauB"
                     /locus_tag="ACHURJ_003742"
     CDS             complement(3144..3911)
                     /gene="tauB"
                     /locus_tag="ACHURJ_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFR1282510.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQVLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLSLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(3924..4886)
                     /gene="tauA"
                     /locus_tag="ACHURJ_003743"
     CDS             complement(3924..4886)
                     /gene="tauA"
                     /locus_tag="ACHURJ_003743"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR1282511.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQAGNT
                     FAKESGASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKLGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKVIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         1185 a         1417 c         1244 g         1119 t
ORIGIN      
        1 tggacatata taatttatgg agaaaaacac gcatcaaatt gataaaagga cgatatttca
       61 acatgttgca gataatgtta tttttgatac atacgcgtta taaatatcaa aaacacaaac
      121 cagataaata tcttatcaat atacaacaaa gtttgaatta ttcaatttat agcagcacaa
      181 ccaaacgcaa aaaaaaaaat aataaccaag agctataaat aaagattaaa aatattttca
      241 gtgaatatga acttattgct atttctatta caaagatgtc ctggtctctc tttcattctc
      301 ttcttgtcaa catcacgaca actttcgtaa aacactcctg ccccgcttca ggtatactgt
      361 gcaccttaat tccacaaaca tcaggcagac catgacagac ttaatccaac gccctcgtcg
      421 cctgcgcaaa tctcctgcgc tgcgcgctat gtttgaagag acaacactta gccttaacga
      481 cctggtgttg ccgatctttg ttgaagaaga aattgacgac tacaaagccg ttgaagccat
      541 gccaggcgtg atgcgcattc cagagaaaca tctggcacgc gaaattgaac gcatcgccaa
      601 cgccggtatt cgttccgtga tgactttcgg catctctcac cataccgatg aaaccggcag
      661 cgacgcctgg cgggaagatg gactggtggc gcgtatgtcg cgcatctgca agcagactgt
      721 gccagaaatg atcgtcatgt cagacacttg tttctgtgaa tacacttctc acggtcactg
      781 cggtgtgctg tgcgagcatg gcgtcgacaa cgacgcgact ctggaaaatt taggcaagca
      841 agccgtggtt gcagctgctg caggcgcaga cttcatcgcc ccttccgccg cgatggacgg
      901 ccaggtacag gcgattcgtc aggcgctgga cgctgcaggc tttaaagata cggcgattat
      961 gtcgtattcg accaagttcg cctcttcctt ttatggcccg ttccgtgaag ctgccggaag
     1021 cgcattaaaa ggcgaccgca aaagctatca gatgaaccct atgaaccgtc gcgaggcgat
     1081 tcgtgagtca ctgctggatg aagcccaggg cgcagactgc ctgatggtta aacctgctgg
     1141 agcgtacctc gacatcgtgc gtgagctgcg tgaacgtact gaattgccga ttggcgcgta
     1201 tcaggtgagc ggtgagtacg cgatgattaa gttcgccgcg ctggcgggtg ctatagatga
     1261 agagaaagtc gtgctcgaaa gcttaggttc aattaagcgt gcgggtgcgg atctgatttt
     1321 cagctacttt gcgctggatt tggctgagaa gaagattctg cgttaatttt tttcctctca
     1381 gaaaggggga gttaacctgt tgaaagtacg gtgaacgccc cctcacccta accctctccc
     1441 caagggggcg agggaaccgt ccgcactcgt atcaccccgc ccgataaaac ggtttatccc
     1501 caagaatcgt cacccgatgc ataatccgcc gctgtggcag gtaatcggca ttggcatagt
     1561 gctgagtcac gcagttatcc caaatcgcaa tatcattcgg ttgccagcgc caacgcacct
     1621 gaaactccgg tttagtgata tgggcgaata aaaagcctaa caaggcttcg ctctctttct
     1681 cgctgacatc gacaattcgc gtagtaaagc cttcattcac aaacagcgcc tgtttaccgc
     1741 tcaccggatg cgttcgcacc accggatgta gcaacggagg atttttcgcg accgcctcgc
     1801 gccagcgttg atgttcctcc tcggttttgc ggtatttgta ttccgggaac gatttacgga
     1861 aatcatgctc cgcacgcagc ccgctcagca gctggcggaa gggaacagag agcgcctcat
     1921 aggccgtaat accgctggtc cagagcgtat caccgccggt cgaaggtaat tctttagctg
     1981 ccagaatcgc ccctgcgggt ggcgtttcaa taaatgtcac atcggtatgc cagttgtcgt
     2041 tatctggcgg attatcgtta tgggtatcca gcacgatgat ctcgtcaacc ccttcggcat
     2101 gagggtaaac agggtgaata tgcagttcgc caaaacgctg ggccagcgcg cgttgctgct
     2161 gcggcgtaat ggcttgatcg cgcagaaaca ccacctgatg gcgcagcacc gcatggtaaa
     2221 gctgttcaaa ctgattatcg cttaacgggc gcgtcaggtc ggcacccgaa atttgtgcgc
     2281 cgatatacgg ccccagcggg gtaatgctca gacgttcact cattgtactt ctccatgcta
     2341 gggcgtcaga cggcgctgta acgcgcgcaa acccagttct aaaagaaagg cgataatcgc
     2401 aatcaccgcg attcccgcca gcaccacgtc agtcgcgaga aattcacccg ctgactgaac
     2461 cataaatcct aaaccgcgcg tcgcggcaat cagctccgcc gctaccagcg tagaccagcc
     2521 cacacccaga ccaatgcgta atccggtgag gatttccggc agcgcgccgg gcaaaatgac
     2581 aaaccacagc acctgcgcac ggctggcacc cagcgactgg gcggcacgaa tgcgaacctg
     2641 ctgcgcgctt ttcacccccg ccagcgccga catcgccacc ggtgcaaaaa tcgctaaata
     2701 gatcagtaag atcttcgagg tttcaccaat accaaaccag atcaccatca gcggcaaata
     2761 agccagcggc gacaccggac gataaagttc gattaccgga tccagaatgc cgcgcaccgt
     2821 cgggcttagc cccatggcga tcccgaccgg aataccgatc accaccgccg cgagtagcgc
     2881 cagcacaatg cgcgtcaggc tggctgccag atgctgccac agcgtggcgt ccataaatcc
     2941 ctgcggtccg gcaatggtga gcagttttgc cagtacttgt tgcggcggcg gcaaaaacag
     3001 cgggctaatc agttgcagcg ccgccaccgt ccaccataca gtgagtaaaa ccgctaacgt
     3061 gccaatgctt aaggtcacct gacgcgagag cggccagcgc catttcagcc gccgcgaatg
     3121 cagtttttca ttaatgagca cactcatgag aacgcctccc gttgctcaaa tacgcggctt
     3181 aaaacatatt cgcgcatggc gatgaattgt ggatcggact tgatgctgcg gctcgactct
     3241 cccgcaacaa agcggcgagc aaagttgagc gacagccgct ccagcacacg gccagggccg
     3301 gatgaaagca gaaccagttc agtcgccata aacaccgctt cttctatatc gtgggtaatc
     3361 aacagcacct gcttgcccgt ctcctgccag agtttcagca gcagagtttg catctggtcg
     3421 cgggtgaagg cgtccagcgc accaaacggt tcgtcgagta ataacagctg gggattcgcc
     3481 gccagcgcgc gggcaatccc cacccgctga cgttgaccac cggaaagctg ccagatatag
     3541 cgtttttctg cgccttccag ccccactttt ttcagcacct ggtgcgcgat ttccagtcgc
     3601 tgcattttct ctatacctgc caattgcagg ccgaacgcca cgttgtcctg tacattgcgc
     3661 cacggtagca gcccttcatt ctgaaaaacc accccgcgct ctgctcccgg tccctcaata
     3721 cgcttacccg ccagttgaat gctgccatgc tgataaggca caaaaccggc aatcagattc
     3781 agcaacgtgg ttttaccgca gccggacggc cccagcacca ccagtagctc gccgctttcc
     3841 agcgtcaggt tgatatcttc cagtgccggt ttgccgccat aatcggcata gagatgagag
     3901 atttgcagca tctgcgcctc cttttattgc acgaagcgcg aggtaacgta ctggctgtaa
     3961 tcattcgcta cagccgggac cttgccctgc tctttcaaaa actgcgcggt gtcgatgatt
     4021 actttgttca ccggtccggt cagttctgcc gtttgttgct gcggcgtcag ataggtattc
     4081 cccttaacca gccccggaac gtcaccttcc ggcacgccgc ttaaacgcgc cagtttgctg
     4141 atgttttccg gctgtttcag ccacgcgtct gggttagcaa tgtacggttg ctgagcatcg
     4201 atggcgcttt tagcgaacgc tttcacgacc tcaggatgtt tctcggcaaa atctttgcgc
     4261 accacccaga cgtccagcgt tggcgcaccc cactgcccga cctgttcaga atcggtcaac
     4321 accttgccgt ctttttccag ggcgttaacc gccggtgccc agacataagc accatcaata
     4381 tcaccccgct gccatgcggc gataatcgcg ggcggctgca ggttcacaat ctccacttgc
     4441 cctagtttaa tcccccagtg tttcagcgcc gccagcaggc tgtagtgggt ggtggagata
     4501 aacggtacgg caatgcgttt gccaatcaaa tcttcgggtt tgctgatagt tttctttacc
     4561 accagcgctt cggagttacc cagttttgac gccagcaaga agacttcaat cggcacctgt
     4621 tggctggctg caaccgctaa tgggctggaa ccgaggttgc cgatttgcac gtcgccagaa
     4681 gccagcgccc gcacgatgct ggctccgctg tcaaacttac gccagtccac gctcgccccg
     4741 ctctctttag caaaggtgtt gccggcctga gccactttcg ccggttcggc tgaggtttga
     4801 tacgccacgg tgacgttcac cgcctgtgcc tgaaaagcga tgaatgccag tgcggcaaga
     4861 agtgtgttac gcgatgaaat tgccatgatt gtctgctccc cagtcttgtt atgggagcag
     4921 tattcaggaa taaaaacatt cattaaaaga attagtcgtt atcgc
//
LOCUS       DAZPEB010000225         4960 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZPEB010000225 DAZPEB010000000
VERSION     DAZPEB010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4960
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.230"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..247)
                     /locus_tag="ACHURJ_003744"
     CDS             complement(<1..247)
                     /locus_tag="ACHURJ_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical enzyme domain-containing protein"
                     /protein_id="HFR1282512.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLAN"
     gene            505..1284
                     /gene="deoC"
                     /locus_tag="ACHURJ_003745"
     CDS             505..1284
                     /gene="deoC"
                     /locus_tag="ACHURJ_003745"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFR1282513.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQTKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            1411..2733
                     /gene="deoA"
                     /locus_tag="ACHURJ_003746"
     CDS             1411..2733
                     /gene="deoA"
                     /locus_tag="ACHURJ_003746"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFR1282514.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDARALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            2785..4008
                     /gene="deoB"
                     /locus_tag="ACHURJ_003747"
     CDS             2785..4008
                     /gene="deoB"
                     /locus_tag="ACHURJ_003747"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFR1282515.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            4088..4807
                     /gene="deoD"
                     /locus_tag="ACHURJ_003748"
     CDS             4088..4807
                     /gene="deoD"
                     /locus_tag="ACHURJ_003748"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFR1282516.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
BASE COUNT         1195 a         1261 c         1364 g         1140 t
ORIGIN      
        1 cgttcgccag aaaacgggcg taatcgggta agacatagcg cggtttgtac ggcgcatgac
       61 cttcaaacat atcgcagatt acaccctcat ccagcgcgcg gcgggcttcg gcaggaagct
      121 gcggataagg cagattgttt tctgcttcca gagccagaaa atggcgcttc tgctccgggc
      181 taagcactgg gctggtgaca atttgctggc aacgttgttg cagtgcattt tcatgagaag
      241 tgggcatctt cttttccttt tatgccgaag gtgatgcgcc attgtaagaa gtttcgtgat
      301 gttaactttg atcctgatgc gtttgccacc actgacgcat tcatttgaaa gtgaattatt
      361 tgaaccagat cgcattacag tgatgcaaac ttgtaagtag attaccttaa ttgtgatgta
      421 tatcgaagtg tgttgcggag tagatgttag aatactaaca aactcgcaag gtgaatttta
      481 ttggcgacaa gccaggagaa tgaaatgact gatctgaaag caagcagcct gcgtgcactg
      541 aaattgatgg acctgaccac cctgaatgac gacgacaccg acgagaaagt aattgctctg
      601 tgtcatcaga ccaaaactcc ggtcggcaat accgccgcta tctgtatcta tcctcgcttt
      661 atcccgattg ctcgcaaaac gctgaaagag cagggcaccc cggaaatccg aatcgctacg
      721 gtaaccaact tcccacacgg taacgatgac atcgaaatcg cgctggcaga aacccgtgcg
      781 gcaatcgcct acggtgctga tgaagttgac gttgtgttcc cgtaccgcgc gctgatggcg
      841 ggtaacgagc aggttggttt tgacctggtg aaagcctgta aagaggcttg cgcggcagcg
      901 aatgtactgc tgaaagtgat catcgaaacc ggcgaactga aagacgaagc gctgatccgt
      961 aaagcgtctg aaatctccat caaagcgggt gctgacttca tcaaaacctc taccggtaaa
     1021 gtggcagtga acgcgacgcc ggaaagcgcg cgcatcatga tggaagtgat ccgtgatatg
     1081 ggcgtagaaa aaaccgttgg tttcaaaccg gcgggcggcg tgcgtactgc ggaagatgcg
     1141 cagaaatatc tcgccatcgc agatgaactg ttcggtgctg actgggcaga tgcgcgtcac
     1201 taccgctttg gtgcttccag cctgctggca agcctgttga aagcgctggg ccacggtgac
     1261 ggtaagagcg ccagcagcta ctaagcaaga tgctttacgc ctgatgcgct gcgcttatca
     1321 ggcctacgag acgtatctac ccgtaggccg gataaggcgt agacgcatcc ggcaaaagcc
     1381 gcctcatact cttttcctcg ggaggttacc ttgtttctcg cacaagaaat tattcgtaaa
     1441 aaacgtgatg gtcatgcgct gagcgatgaa gaaattcgtt tctttatcaa cggcattcgc
     1501 gacaacacta tctccgaagg gcagattgcc gccctcgcga tgaccatttt cttccacgat
     1561 atgacaatgc ctgagcgtgt ctcgctgacc atggcgatgc gagattcagg aaccgttctc
     1621 gactggaaaa gcctgcatct gaatggcccg attgttgata aacattccac cggcggcgtc
     1681 ggcgatgtca cttcgctgat gttggggccg atggtcgcag cctgcggcgg ctatattccg
     1741 atgatctccg gtcgcggcct cggtcatact ggcggtacgc tcgacaaact ggaatccatc
     1801 cctggcttcg acattttccc ggatgacaac cgtttccgcg aaattattaa agacgtcggc
     1861 gtggcgatta tcggtcagac cagctcactg gctccggcgg ataaacgttt ctacgcgacc
     1921 cgtgatatta ccgcaaccgt ggactccatc ccgctgatca ctgcctcgat cctggcgaag
     1981 aaactggcgg aaggtctgga tgcgctggtg atggacgtga aagtgggtag cggcgcgttt
     2041 atgccgacct acgaactctc tgaagccctt gccgaagcga ttgttggcgt ggctaacggc
     2101 gctggcgtgc gcaccaccgc gctgctcacc gacatgaatc aggtactggc ctccagtgca
     2161 ggtaacgcgg ttgaagttcg tgaagcggtg cagttcctga cgggtgaata tcgtaacccg
     2221 cgtctgtttg atgtcacgat ggcgctgtgc gtggagatgc tgatctccgg caaactggcg
     2281 aaagatgacg ccgaagcgcg cgcgaaattg caggcggtgc tggacaacgg taaagcggca
     2341 gaagtctttg gtcgtatggt agcggcacaa aaaggcccga ccgacttcgt tgagaactac
     2401 gcgaagtatc tgccgacagc gatgctgacg aaagcagtct atgctgatac cgaaggtttt
     2461 gtcagtgaaa tggatgcccg cgcgctgggg atggcagtgg ttgcaatggg cggcggtcgt
     2521 cgtcaggcat ctgacaccat cgattacagc gtcggcttta ctgatatggc gcgtctgggc
     2581 gaccaagtag acggtcagcg tccgctggcg gttatccacg cgaaagacga aaacagctgg
     2641 caggaagcgg cgaaagcggt gaaagcggca attaaacttg ccgataaagc accggaaagc
     2701 acaccaactg tctatcgccg tatcagcgaa taacggtata ctgatctgat catttaaatt
     2761 tgaagcactg agtacggaga acatatgaaa cgtgcattta ttatggtgct ggactcattc
     2821 ggcatcggcg ctacagaaga tgcagaacgc tttggtgacg tcggggctga caccctgggt
     2881 catatcgcag aagcttgtgc caaaggcgaa gctgataacg gtcgtaaagg cccgctcaat
     2941 ctgccaaatc tgacccgtct ggggctggcg aaagcacacg aaggttctac cggtttcatt
     3001 ccggcgggaa tggacggcaa cgctgaagtt atcggcgcgt acgcatgggc gcacgaaatg
     3061 tcatccggta aagatacccc gtctggtcac tgggaaatcg ccggtgtccc ggttctgttt
     3121 gagtggggat acttctccga tcacgaaaac agcttcccgc aagagctgct ggataaactg
     3181 gtcgaacgcg ctaatctgcc gggttacctc ggtaactgcc actcttccgg tacggtcatt
     3241 ctggatcagc tgggcgaaga gcacatgaaa accggcaagc cgattttcta tacctccgct
     3301 gactccgtgt tccagattgc ctgccacgaa gaaacctttg gcctggataa actctacgaa
     3361 ctgtgcgaaa tcgcccgtga agagctgacc aacggcggct acaacatcgg tcgtgttatc
     3421 gctcgtccgt ttatcggtga caaagccggt aacttccagc gtaccggtaa ccgtcacgac
     3481 ttggctgttg agccgccagc accgaccgtg ctgcagaaac tggttgatga aaaacacggt
     3541 caggtggttt ctgtcggtaa aattgcagac atctacgcca actgcggtat caccaagaaa
     3601 gtgaaagcaa ctggcctgga cgcgctgttt gacgccacca tcaaagagat gaaagaagcg
     3661 ggtgataaca ccatcgtctt caccaacttc gttgacttcg actcttcctg gggccaccgt
     3721 cgcgacgtcg ccggttatgc tgcgggtctg gagctgttcg accgccgtct gccggagctg
     3781 atgtctttgc tgcgcgatga cgacatcctg atcctcactg ctgaccacgg ttgtgatccg
     3841 acctggaccg gtactgacca cacgcgtgaa cacattccgg tactggttta cggcccgaaa
     3901 gtaaaaccgg gttcactggg tcaccgtgaa accttcgcgg atatcggcca gactctggca
     3961 aaatattttg gtacttctga tatggaatat ggcaaagcca tgttctgata atgggtgcgg
     4021 cttgatgccc tcaccccggc cctctcccac agggagaggg tgaaaacatg ttgataagga
     4081 taaaacaatg gctaccccac acattaatgc agaaatgggc gatttcgctg acgtagtttt
     4141 gatgccaggc gacccgctgc gtgcgaagta tattgctgaa actttccttg aagatgcccg
     4201 tgaagtgaac aacgttcgcg gtatgctggg cttcaccggt acttacaaag gccgcaaaat
     4261 ttccgtaatg ggtcacggta tgggtatccc gtcctgctcc atctacacca aagaactgat
     4321 caccgatttc ggcgtgaaga aaattatccg cgtgggttcc tgtggcgcag ttctgccgca
     4381 cgtaaaactg cgcgacgtcg ttatcggtat gggtgcctgc accgattcca aagttaaccg
     4441 catccgtttt aaagatcatg actttgccgc tatcgctgac tttgacatgg tgcgtaacgc
     4501 ggtagacgcg gctaaagcac tgggcattga tgctcgcgtt ggtaacctgt tctccgctga
     4561 cctgttctac tctccggacg gcgaaatgtt cgacgtgatg gaaaaatacg gcatcctcgg
     4621 cgtggaaatg gaagcggctg gtatctacgg cgtcgctgca gaatttggcg cgaaagccct
     4681 gaccatctgt accgtgtctg accacatccg cactcacgag cagaccactg ccgctgagcg
     4741 tcagaccacc ttcaacgaca tgatcaaaat cgcactggaa tccgttctgc tgggcgataa
     4801 agagtaattg tgtttcgctg aaaggcgatt gtcgtgtgaa gccggagtgg gagactgctc
     4861 cggcttttta gtatctattc atcaatagca actgatttat agatttacta ttatttttca
     4921 tatggataga aaataaaatc tattcaagta tctattcacg
//
LOCUS       DAZPEB010000226         4883 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZPEB010000226 DAZPEB010000000
VERSION     DAZPEB010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4883
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.231"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..801
                     /locus_tag="ACHURJ_003749"
     CDS             <1..801
                     /locus_tag="ACHURJ_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA family autotransporter adhesin"
                     /protein_id="HFR1282517.1"
                     /translation="QSITNLNNQVTNLDTRVTNIENGIGDIVTTGSTKYFKTNTDGVD
                     ANAQGKDSVAIGSGSIAAADNSVALGTGSVAEEENTISVGSSTNQRRITNVAAGVNAT
                     DAVNVSQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGSTTRISNVSAGVNNNDAVNY
                     AQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAMTGLPQAYTPGASMASI
                     GGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAALGAGIQW"
     gene            1169..2824
                     /gene="lldP"
                     /locus_tag="ACHURJ_003750"
     CDS             1169..2824
                     /gene="lldP"
                     /locus_tag="ACHURJ_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFR1282518.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            2824..3600
                     /gene="lldR"
                     /locus_tag="ACHURJ_003751"
     CDS             2824..3600
                     /gene="lldR"
                     /locus_tag="ACHURJ_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFR1282519.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            3597..4787
                     /gene="lldD"
                     /locus_tag="ACHURJ_003752"
     CDS             3597..4787
                     /gene="lldD"
                     /locus_tag="ACHURJ_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFR1282520.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADTHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPAALAPMAK
                     GNAA"
BASE COUNT         1071 a         1304 c         1319 g         1189 t
ORIGIN      
        1 cagagcatta ctaacctgaa caatcaggtc actaatctgg atactcgcgt tactaatatc
       61 gaaaacggta ttggcgacat tgtcaccacc ggtagcacca agtacttcaa gaccaacacc
      121 gatggcgtag atgccaacgc ccagggtaaa gatagcgttg ctattggttc tggttccatt
      181 gctgccgctg acaacagcgt cgcactgggt accggttccg ttgcagagga agaaaataca
      241 atctctgtag gttcttctac taaccaacgc cgtatcacca acgtagctgc cggtgttaat
      301 gccaccgatg cggttaacgt gtcgcagctg aaatcttctg aagcaggcgg cgtgcgctac
      361 gacaccaaag ctgatggttc tatcgactat agcaatatca ccctcggtgg tggcaacggc
      421 agtacgactc gtatcagcaa cgtctccgct ggcgtcaaca acaacgacgc ggtgaactac
      481 gcgcagttga agcaaagcgt gcaggaaacg aagcaataca ccgatcagcg gatggttgag
      541 atggataaca aactgtctaa aactgaaagc aagttgagcg gtggtatcgc ttctgcaatg
      601 gcaatgaccg gtctgccgca ggcttataca ccgggtgcca gcatggcttc tattggtggc
      661 ggtacttaca acggtgaatc ggcagttgct ttaggtgtat cgatggtgag cgccaatggt
      721 cgttgggtct acaaattaca aggtagtacc aatagccagg gtgaatactc cgccgcactc
      781 ggtgccggta ttcagtggta atgatccatt aagttagtgt gactaagggc aaacgccaaa
      841 ttgcctgatg cgttacgcgc atcaggcaag ataaggcgct ccgggtcaac aatctaaggg
      901 caattctctg atgaggattg cccttttctt taccagacat ctccccccac aagaattggc
      961 cctaccaatt cttcgcttat ctgacctctg gttcacaatt ccccaattaa aactcacatc
     1021 aatattgcca atacacaaca tttagttaac cattcattgt cattatccct acacaacaca
     1081 attggcagtg ccacttttac acaacgtgtg acaaggagat gagcaacaga ctcattacac
     1141 gatgtgcgtg gactccagga gacctgcaat gaatctctgg caacaaaact acgatcccgc
     1201 cgggaatatc tggctttcca gtctgatagc atcgcttccc atcctgtttt tcttctttgc
     1261 gctgattaag ctcaaactga aaggatacgt cgccgcctca tggacggtag caatcgccct
     1321 tgccgtggct ttgctgttct ataaaatgcc ggtcgctaac gcgctggcct cggtggttta
     1381 tggcttcttc tatggtttgt ggcccatcgc gtggatcatt attgcagcag tgttcgtcta
     1441 taagatctcg gtgaaaaccg ggcagtttga catcattcgc tcgtctattc tttcgataac
     1501 ccctgaccag cgtctgcaaa tgctgatcgt cggtttctgt ttcggcgctt tccttgaagg
     1561 agccgcaggc tttggcgcac cggtagcaat taccgccgca ttgctggtcg gcctgggttt
     1621 taaaccgctg tacgccgccg gactgtgcct gattgttaac accgcgccag tggcatttgg
     1681 cgcgatgggc attccaatcc tggttgccgg acaagtaaca ggcatcgaca gttttgagat
     1741 tggccagatg gtggggcgtc agctaccgtt tatgaccatt atcgtgctgt tctggatcat
     1801 ggcgattatg gacggctggc gcggtatcaa agagacgtgg cctgcggtcg tggttgcggg
     1861 cggctcgttt gccatcgctc agtaccttag ctccaacttc attgggccgg agctgccgga
     1921 catcatctct tcgctggtat cactgctctg cctgacgctg ttcctcaaac gctggcaacc
     1981 agttcgcgta ttccgcttcg gtgacttggg ggcgtcacag gttgatatga cgctggccca
     2041 caccggttac accgcgggtc aggtgttacg tgcctggaca ccgttcctgt tcctgaccgc
     2101 taccgtaaca ctgtggagca tcccgccgtt taaagccctg ttcgcatcgg gtggtgcgct
     2161 gtatgagtgg gtgatcaaca ttccggtgcc gtacctcgat aaactggttg cccgtatgcc
     2221 accagtggtc agcgaggcta cagcctatgc cgccgtgttt aagtttgact ggttctctgc
     2281 caccggcacc gccattctgt ttgctgcact gctctcgatt gtctggctga agatgaaacc
     2341 gtctgacgct atcagcacct tcggcagcac gctgaaagaa ctggctctac ccatctactc
     2401 catcggcatg gtgctggcgt tcgcctttat ctcgaactat tccggactgt catcaacact
     2461 ggcactggca ctggcgcaca ccggtcatgc attcaccttc ttctcgccgt tcctcggctg
     2521 gctgggggta ttcctgaccg ggtcggatac ctcatctaac gccctgttcg ccgcgctgca
     2581 agccaccgca gcacaacaaa ttggcgtctc tgatctgttg ctggttgccg ccaataccac
     2641 cggtggcgtc accggtaaga tgatctcccc gcaatctatc gctatcgcct gtgcggcggt
     2701 aggcctggtg ggcaaagagt ctgatttgtt ccgctttact gtcaaacaca gcctgatctt
     2761 cacctgtatg gtgggcgtga tcaccacgct tcaggcttat gtcttaacgt ggatgattcc
     2821 ttaatgattg ttttacccag acgcctgtca gacgaggttg ccgatcgtgt gcgggcgctg
     2881 attgatgaaa aaaacctgga agcgggcatg aagttgcccg ctgagcgcca actggcgatg
     2941 caactcggcg tatcacgtaa ttcactacgc gaggcgctgg caaaactagt gagcgaaggc
     3001 gtgctgctca gtcgacgcgg cggcgggacg tttattcgct ggcgtcatga cacatggtcg
     3061 gagcaaaaca tcgtccagcc gctaaaaacg ctgatggccg atgatccgga ttacagtttc
     3121 gatattctgg aagcccgcta cgccattgaa gccagcaccg catggcatgc ggcaatgcgc
     3181 gccacacctg gcgacaaaga aaagattcag ctttgctttg aagcaacgct aagtgaagac
     3241 ccggatatcg cctcacaagc ggacgttcgt tttcatctgg cgattgccga agcctcacat
     3301 aacatcgtgc tgctgcaaac catgcgcggt ttcttcgatg tcctgcaatc ctcagtgaag
     3361 catagccgtc agcggatgta tctggtgcca ccggtttttt cacaactgac cgaacaacat
     3421 caggctgtca ttgacgccat ttttgctggt gatgccgacg gggcgcgtaa agcaatgatg
     3481 gcgcacctta gttttgttca caccaccatg aaacgattcg atgaagatca ggctcgccac
     3541 gcacggatta cccgcctgcc cggtgagcat aatgagcatt cgagggagaa aaacgcatga
     3601 ttatttccgc agccagcgat tatcgcgccg cagcgcaacg cattctgccg ccgttcctgt
     3661 tccactatat ggatggtggt gcatattctg aatacacgct gcgccgcaac gtggaagatt
     3721 tgtcagaagt ggcactgcgc cagcgtattc tgaaaaacat gtccgactta agcctggaaa
     3781 cgacgctgtt taatgagaaa ttgtcgatgc cggtagcact ggcaccggtg ggtttgtgtg
     3841 gcatgtatgc gcgtcgtggc gaagttcagg cagccaaagc ggcggacacg catggcattc
     3901 cgtttactct ctcgacggtt tccgtttgcc cgattgaaga agtcgcgcca gccatcaagc
     3961 gcccaatgtg gttccagctt tatgtactgc gcgatcgcgg ctttatgcgt aacgcgctgg
     4021 agcgagcaaa agcagcgggt tgttcgacgc tggttttcac cgtggatatg ccgacaccgg
     4081 gcgcacgcta ccgtgatgcg cattcaggta tgagcggccc aaatgcggca atgcgccgct
     4141 acttgcaggc ggtgacgcat ccgcaatggg cgtgggatgt gggcctgaac ggtcgtccac
     4201 atgatttagg taatatctca gcttatctcg gcaaaccaac cggactggaa gattacatcg
     4261 gctggctggg gaataacttc gatccgtcca tctcatggaa agaccttgag tggatccgcg
     4321 atttctggga tggcccgatg gtgatcaaag ggatcctcga tccggaagat gcgcgcgatg
     4381 cagtacgttt tggtgctgat ggaattgtgg tttctaacca cggtggccgc cagctggacg
     4441 gtgtactctc ttccgctcgt gcgttgcccg ctattgcaga tgcggtgaaa ggtgatatcg
     4501 ccattctggc ggatagcgga attcgtaacg ggcttgatgt cgtgcgtatg attgcgctcg
     4561 gtgccgacac cgtactgctg ggtcgtgctt tcctgtatgc gctggcaaca gcgggccagg
     4621 cgggtgtagc taacctgcta aatctgatcg aaaaagagat gaaagtggcg atgacgctga
     4681 ctggcgcgaa atcgatcagt gaaattacgc aagattcgct ggtgcaaggg ctgggtaaag
     4741 agttgcctgc ggcactggct cccatggcga aagggaatgc ggcatagtcg tttgcgcccc
     4801 cgttacttta ttcgtacccc ttataatggg gtgttagcca gccagacccg gcatgattac
     4861 tgcccccagt cgtccatgat ccg
//
LOCUS       DAZPEB010000227         4882 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZPEB010000227 DAZPEB010000000
VERSION     DAZPEB010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4882
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.232"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..601
                     /gene="parM"
                     /locus_tag="ACHURJ_003753"
     CDS             <1..601
                     /gene="parM"
                     /locus_tag="ACHURJ_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="plasmid segregation protein ParM
                     domain-containing protein"
                     /protein_id="HFR1282521.1"
                     /translation="KKANVMRPVEYQNGEAFTIRNVQVMPESIPAGFKALADMSPFES
                     LLIVDLGGTTLDVAKVQGQLAGISQVFCDPHVGVSLMADAVLSVMATNGMRTSHHIAN
                     TIIEHRHDEAWLRQHIHNDAHYASLMAVIREKEETLKQRVIRALAVFSGYGRVMVVGG
                     GAEIVAPAIREACGVNATFIADGVPQFALVNGLYAMDKE"
     gene            606..917
                     /locus_tag="ACHURJ_003754"
     CDS             606..917
                     /locus_tag="ACHURJ_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012907691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFR1282522.1"
                     /translation="MTTPTRRISFYLKPTAVKNEGEACAWLDSLTPEARKSGQRVAFL
                     AGLALLKMNPAEAYRLAAWAGDEALSVTQTRTERPASQPVSTAQITSQMAGNIRALFP
                     E"
     gene            981..1571
                     /locus_tag="ACHURJ_003755"
     CDS             981..1571
                     /locus_tag="ACHURJ_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFR1282523.1"
                     /translation="MSEINYQALRERYSPVPVPKCPICGEEMSIQRISGAQVVYACSG
                     YGDDGDFKIGRTLADEHYEKSRVTVLDVGDPEVLALLDWLETKDNRIAELEKIATDYA
                     LKFQKAQDALKHATLLHSRTAQQTNNFAVSLPDISEYFINDVFQPLRYERDVERAIIK
                     AGGKALWQEKHEDRTHQSCDVNCGWFSPLTTDKNNT"
     gene            1865..1993
                     /locus_tag="ACHURJ_003756"
     CDS             1865..1993
                     /locus_tag="ACHURJ_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282524.1"
                     /translation="MKGKAVKIALRRREHAVNAVGLRKSLTVDGGDKLEGVVKLSD"
     gene            complement(2062..3108)
                     /locus_tag="ACHURJ_003757"
     CDS             complement(2062..3108)
                     /locus_tag="ACHURJ_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR1282525.1"
                     /translation="MSRKNKKVRMSSRIDLADALRKESSLSAFTFDGPYRLTGHDLLD
                     NMYCADNGRWYETPVDWYGLARAARQTSWHQSALYFKRNVLLGCYIPHPLLSRQDFSA
                     LALDWFVFGNAFLELRSNMLGEPLKLRHALAKYMRRGSDLESWRYVQDGKDAFQFRPG
                     KVCHLMNPDINQEIYGMPEYLGALLSASLSHSADMFRKLYYDNGSHAGCIIYIGAAQV
                     NRESMDSLKETLQGARGGGAFKNVLIHAPNGGKEGVQILPFQQITAKDEFMNVKAASR
                     DDVLAAHRVPPQLMGAMPGEKSAFGDVEKAARVYAINELMPVMEAMKHINDWLGEEVI
                     RFNPYALLDIQPTS"
     gene            complement(3108..4859)
                     /locus_tag="ACHURJ_003758"
     CDS             complement(3108..4859)
                     /locus_tag="ACHURJ_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFR1282526.1"
                     /translation="MIQDAFVRQRARQLYWQGYPPAEISRLMGINPNTIYAWKKRDQW
                     DETPPGQRVTQSIDARLIQLTEKQNKTGGDFKEIDLLTRQLKKLHDGQPDVMAAGKKG
                     RAKKLKNHFTPEQIAALREKIISRLEWHQRGWFDSLTLCREAGIRNRMILKSRQIGAT
                     WYFAQEALLMALRDDVAQPYQRNQIFLSASRRQAFQFKSIIQKAAAEVDVELKGGDKI
                     ILSNGAELHFLGTSAASAQSYTGNFYFDEFFWVSRFAELRKVAGAMATLSGLRRTYFS
                     TPSTETHEAYAYWNGDRWNEKKASHKRQRFSVDWKTLHNGLICPDRTWRQIVTLEDVV
                     NHGWKHTDIDEIRDENTEDEFLNLYMCEFVREGESAFNLNILIGCGVDGYDDWKDWKP
                     FAPRPMGNRPVWIGYDANGSSGNGDSGAVSVVVPPSVPGGRFRTVETRRVQGLEFEEQ
                     ARVIEEFTCRYNVEHIGIDVTGGNGEAVYQIVKRFFPAAIPYTFTLSSKRSLVLKMLQ
                     IMRAGRWEYDRAERELVAAFNAVRKVKTPGGFITYETDRARGISHGDLAWATMLAVIN
                     EPIGGEGENERFTVMEF"
BASE COUNT         1175 a         1321 c         1268 g         1118 t
ORIGIN      
        1 caaaaaagcg aacgtcatgc gcccggtgga gtaccagaac ggcgaagcat tcactatccg
       61 taacgtacag gttatgcctg aatccattcc ggctggcttt aaggcactgg ctgacatgag
      121 tccgtttgaa tccctgctga ttgtggattt gggcggaacc acgctggatg tggcaaaggt
      181 tcaggggcaa ctggcaggta tcagccaggt gttttgcgat ccacacgtgg gcgtttctct
      241 gatggccgat gccgtactgt cggtgatggc cactaacggt atgcgcacca gtcaccacat
      301 cgccaatacc attatcgaac atcgccatga tgaagcctgg ctgcgccagc acatccacaa
      361 tgacgcgcat tacgccagcc tgatggcggt aattcgtgaa aaggaagaaa cactgaaaca
      421 acgcgtgatc cgcgcgctgg cggttttttc gggttacggg cgggtgatgg ttgtcggtgg
      481 aggggcggag attgtggcac ccgctatccg cgaagcctgc ggagttaatg cgactttcat
      541 cgcggacggg gtgccacagt ttgctctggt taatgggctg tacgcaatgg acaaggagta
      601 aaccaatgac gactccaacc agacggataa gtttctatct gaagcccacc gccgtcaaga
      661 acgaaggaga agcatgcgcc tggctggaca gccttacacc agaagcccgc aaaagcggcc
      721 aacgcgtggc ttttctggcc gggctggcac ttctgaaaat gaatccggca gaggcttatc
      781 gactggctgc atgggctggt gatgaagcgt tatcagtgac acaaaccagg acagaacgcc
      841 ccgcatcaca gccagtatca accgcacaga taaccagtca gatggccgga aatatccggg
      901 cgttatttcc tgaataacac aacatcaggg cgagttcgcc ctgctctcca cctcagaaca
      961 taaacaagga gaacgactta atgagcgaaa tcaactatca ggcattacgt gaacgttatt
     1021 cacctgtgcc agtaccgaaa tgccctattt gcggcgagga aatgtcaatt cagcgaatat
     1081 ctggagcaca ggttgtttat gcctgctccg gttatggtga tgatggagat ttcaaaattg
     1141 gccgaactct tgctgacgaa cattatgaaa aatcacgcgt aacagtgttg gatgtcgggg
     1201 atcctgaagt attggcgcta cttgattggc tggaaaccaa agacaaccga atcgctgaac
     1261 tggaaaaaat cgccactgac tatgcactta aattccagaa agcacaggac gcattaaagc
     1321 atgccacttt gctgcatagc aggacggcgc aacaaacaaa taattttgca gtatcgcttc
     1381 cggacataag cgaatatttc attaatgacg tatttcagcc cttgcgatac gagcgggatg
     1441 ttgaaagagc catcataaag gctggcggaa aagcattgtg gcaggagaaa cacgaggaca
     1501 gaacgcatca gtcatgcgat gtaaattgtg gatggtttag cccactgacg acagataaaa
     1561 ataacacctg atccccctca aaccagggcg ataatcgcac atcgccctgc tgcacaatag
     1621 tgcacaaatt tgcacaattt ttttgaacga atttttgccc ttccagcccg cgtggcggct
     1681 ggatccgtca aggatccgtg cgtgcacaaa aaaacgcgct ttttctgcgc gcaggtgacg
     1741 ggggaacagc ccgcgtttca gggggtaaat agcgttccct taacgatgtc gcagcgacac
     1801 gacagaatgg ccgtatttct cacgctgagc gtgaaaaata cgtgagggat tctgatttga
     1861 tggggtgaaa ggtaaggccg tcaaaatcgc actgaggcgg cgagaacatg cagtcaacgc
     1921 ggtgggattg cgtaagagtc tgaccgtcga tggtggcgat aaactggaag gcgtcgtgaa
     1981 attatctgac tgatacagga gctggagagt cggggcataa attttttatg ccccggcgaa
     2041 gcagcagaca agcgaagcgc gtcaggatgt gggctggatg tctaacagtg cgtaagggtt
     2101 aaagcggatc acctcttcgc caagccagtc attgatgtgc ttcatggcct ccatgacggg
     2161 catcagctcg ttaattgcgt aaacccgcgc ggccttctcc acatcaccaa acgcactttt
     2221 ttcacccggc atcgccccca tcagttgcgg cggaacgcgg tgcgcagcca gcacatcatc
     2281 acgggatgcc gccttaacat tcatgaactc atcctttgcg gtgatctgct ggaacggcaa
     2341 aatttgcacc ccctctttgc ccccgttggg cgcatgaatg agcacgtttt taaacgcacc
     2401 accaccacgc gcaccctgta gcgtttcttt cagggagtcc atgctttcgc ggtttacctg
     2461 cgcggcaccg atgtagatga tgcacccggc gtgggatccg ttgtcgtaat acagttttct
     2521 gaacatgtcc gccgaatgag acaggctggc cgagagtaat gcgccgagat attccggcat
     2581 gccgtagatt tcctggttaa tgtcaggatt catcaggtga cacactttgc ctgggcgaaa
     2641 ctgaaacgca tccttgccat cctgcacata cctccatgat tcaagatcgc ttccgcgtcg
     2701 catgtatttc gccagggcgt gccgtaattt aagcggttcg ccgagcatat tgcttcgaag
     2761 ctcaaggaat gcgttaccga acacaaacca gtccagcgcc agcgccgaga aatcctgccg
     2821 ggaaagcagc gggtgcggga tgtagcaacc gagcaataca ttgcgcttaa aataaagcgc
     2881 agactgatgc caggacgttt gccgggcagc tcttgccaga ccgtaccagt ccaccggggt
     2941 ttcataccac cgcccgttat cagcacagta catattgtcc agcaggtcat gcccggtcag
     3001 gcgataagga ccatcaaatg tgaatgcact gagcgatgat tctttcctga gcgcatcagc
     3061 gagatcaatg cgtgaactca tgcgcacttt tttatttttt ctgctcatca gaactccata
     3121 accgtgaaac gctcgttttc tccttcgccg ccaatcggtt cgttaatgac agcaagcatg
     3181 gttgcccacg caaggtcgcc gtggctgatc cccctcgctc ggtccgtttc gtaagtgata
     3241 aagccgcccg gtgttttcac cttacgcacg gcgttaaagg ccgcgaccag ctcgcgttcg
     3301 gcgcgatcgt attcccaccg cccggcacgc attatttgca gcattttcag taccagcgac
     3361 cgttttgatg acagcgtgaa ggtgtacgga atagcggcag ggaaaaaccg tttcactatc
     3421 tgataaacag cctccccgtt cccgcccgtc acatcaatgc cgatgtgttc cacgttgtag
     3481 cgacacgtga actcttcaat gactctggcc tgttcttcaa actccagccc ctgaacgcgt
     3541 cgcgtctcca ccgttcgaaa acggccacca ggaacagacg gaggaaccac cacggacaca
     3601 gcgccgctgt cgccgttgcc actgctgccg tttgcgtcat acccaatcca taccggacga
     3661 ttccccatcg ggcggggagc aaaaggtttc cagtctttcc agtcgtcgta tccgtcaacg
     3721 ccgcagccaa tcaggatatt caggttaaat gccgattccc cttcgcggac aaactcacac
     3781 atatagagat tgaggaactc gtcttcggtg ttttcatcac gaatttcgtc gatatcggtg
     3841 tgtttccagc cgtgattaac cacatcttcc agcgtgacaa tttgccgcca cgtccggtca
     3901 gggcagataa gcccgttatg cagcgttttc cagtccacag aaaaacgctg gcgtttatgc
     3961 gaggcctttt tctcgttcca gcggtcgccg ttccagtagg cgtatgcctc gtgcgtttcg
     4021 gtggatggcg tggagaagta ggtgcgccgc agtccgctga gggttgccat agcgccagcc
     4081 accttgcgca gttcagcaaa gcgactgacc cagaaaaatt catcaaaata aaaattgccc
     4141 gtataggact gtgccgacgc agcagaagtg ccgagaaaat gcagctctgc gccgttggag
     4201 aggatgattt tatcgccccc tttcagctcc acatcaactt cagccgcggc cttctgaata
     4261 atgcttttaa actggaacgc ctgacgacgc gacgcagaca aaaaaatctg gttacgctgg
     4321 taaggttgcg ccacatcgtc acgcagcgcc atcagcagtg cttcctgtgc aaaataccag
     4381 gtcgccccaa tctgtcggga tttcaggatc atcctgttac gtatcccggc ttccctgcaa
     4441 agggtcaggg agtcaaacca gccccgctga tgccactcca gcctgctgat gattttttcc
     4501 cgcagtgcgg caatctgttc cggcgtgaaa tgatttttga gttttttcgc ccggcctttc
     4561 tttcctgcgg ccatcacatc cggctggcca tcatgcagct ttttaagctg ccgggtcagc
     4621 aggtctattt ccttaaagtc accgcctgtt ttattctgtt tttcagtaag ctggatgagg
     4681 cgcgcatcga tggactgcgt gacacgctgc ccgggtggcg tttcatccca ctggtcacgt
     4741 tttttccacg cataaatcgt gttcgggttt attcccatca gacgtgatat ttctgcgggc
     4801 ggataaccct gccagtaaag ttgccgcgca cgctggcgca caaaagcgtc ctgaatcatt
     4861 gctccccctg agtaattaca gg
//
LOCUS       DAZPEB010000228         4854 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZPEB010000228 DAZPEB010000000
VERSION     DAZPEB010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4854
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.233"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..1251)
                     /locus_tag="ACHURJ_003759"
     CDS             complement(<1..1251)
                     /locus_tag="ACHURJ_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFR1282527.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPRAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SMVICVINIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNDTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNTPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMA"
     gene            1530..2483
                     /gene="tal"
                     /locus_tag="ACHURJ_003760"
     CDS             1530..2483
                     /gene="tal"
                     /locus_tag="ACHURJ_003760"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFR1282528.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPTLLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            2598..3185
                     /gene="mog"
                     /locus_tag="ACHURJ_003761"
     CDS             2598..3185
                     /gene="mog"
                     /locus_tag="ACHURJ_003761"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFR1282529.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSVLTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(3220..3786)
                     /gene="satP"
                     /locus_tag="ACHURJ_003762"
     CDS             complement(3220..3786)
                     /gene="satP"
                     /locus_tag="ACHURJ_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFR1282530.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YSGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(3935..4648)
                     /gene="msyB"
                     /locus_tag="ACHURJ_003763"
     CDS             complement(3935..4648)
                     /gene="msyB"
                     /locus_tag="ACHURJ_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFR1282531.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAFDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            complement(4674..>4854)
                     /locus_tag="ACHURJ_003764"
     CDS             complement(4674..>4854)
                     /locus_tag="ACHURJ_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFR1282532.1"
                     /translation="ARSASQSLNIPSEIKEGQTTDWININSDNDNKRCVSKITFSGHT
                     VNSSDMATLKIIGDD"
BASE COUNT         1275 a         1250 c         1230 g         1099 t
ORIGIN      
        1 ggccattatg atatctgcca gttgccacat cagcggaagg cttagcaagg tgccgccgat
       61 gaccgttgcg aaggtgcaga tccgcaaaca ccagatcgct ttaggggtgt tcaggcgtaa
      121 aaagaagaga ttgttttcgg cataaatgta gttggcaacg atggagctga aggcaaacag
      181 aataaccacg agggtaacaa actcagcacc ccaggaaccc attagcaccc gcatcgcctt
      241 ctggataagc tgaatacctt ccagcggcat gtaggttgtg tcgttacccg ccagtaatat
      301 cagcatggcg cttgccgtac agatgaccag ggtgtcgata aaaatgccaa tcatctggac
      361 aatcccttgc gctgccggat gcggaggcca ggacgccgct gccgctgccg cgtttggcgt
      421 cgaacccatt cccgcctcat tggaaaacat actgcgctga aaaccgttag taatcgcctg
      481 gcttaaggta tatcccgccg cgccgcctgc cgcttcctgc cagccaaaag cactctcaaa
      541 aatagaccaa atgacgtggg gaagttgccc gatatttatt acgcaaatca ccatgctggt
      601 cagcacccag attatcgcca tcaacgggac aaacccctgc atgagccggg cgacgccatg
      661 aagaccgcga gtgattgcca gcagagcaaa gacagcgaga ataatgcctg tcaccagcgg
      721 gggaaaatca aaagaaaaac tcagggctcg ggcaacggcg ttcgcctgaa ctccgctgaa
      781 aattatgccg taggcgatga gcaaaaagac ggcgaacaga acgcccatcc aacgcatccc
      841 cagcccgcgc gccatatacc atgccggtcc gccacgaaac tgcccattga cgtcacgttc
      901 tttataaagt tgtgcaaggg aacattcggc aaacgaggtc gccatgccga taaacgcggc
      961 aacccacatc caaaagacgg ctctaggtcc accggcggta atagccagcg caacgccggc
     1021 caggttgccg ctacccacgc gcgccgcaag actggtacac aatgactgaa atgaggttaa
     1081 accgcctggc tgtggatgaa tgctattttt aagacttttg ccaaactggc ggatgtagcg
     1141 aaactgcaca aatccggtgc gaaaagtgaa ccaacaacct gcgccgaaga gcaggtaaat
     1201 cattaccgat ccccaaagga cgctgttaat aaaggagaaa aaatctggca tgcatatccc
     1261 tcttattgcc ggtcgcgatg actttcctgt gtaaacgtta ccaattgttt aagaagtata
     1321 tacgctacga ggtacttgat aacttctgcg tagcatacat gaggttttgt ataaaaatgg
     1381 cgggcgatat caacgcagtg tcagaaatcc gaaacagtct cgcctggcga taaccgtctt
     1441 gtcggcggtt gcgctgacgt tgcgtcgtga tatcatcagg gcagaccggt tacatccccc
     1501 taacaagctg tttaaagaga aatactatca tgacggacaa attgacctcc cttcgtcagt
     1561 acaccaccgt agtggccgac actggggaca tcgcggcaat gaagctgtat caaccgcagg
     1621 atgccacaac caacccttct ctcattctta acgcagcgca gattccggaa taccgtaagt
     1681 tgattgatga tgctgtcgcc tgggcgaaac agcagagcaa cgatcgcgcg cagcagatcg
     1741 tggacgcgac cgacaaactg gcagtaaata ttggtctgga aatcctgaaa ctggttccgg
     1801 gtcgtatctc aactgaagtt gatgcgcgtc tttcctatga caccgaagcg tcaattgcga
     1861 aagcaaaacg cctgatcaaa ctctacaacg atgctggtat tagcaacgat cgtattctga
     1921 tcaaactggc ttctacctgg cagggtatcc gtgctgcaga acagctggaa aaagaaggca
     1981 tcaactgtaa cctgaccctg ctgttctcct tcgctcaggc tcgtgcttgt gcggaagcag
     2041 gcgtgttcct gatctcgcca tttgttggcc gtattcttga ctggtacaaa gcgaatactg
     2101 ataagaaaga gtacgctccg gcagaagatc cgggcgtggt ttctgtatct gaaatctacc
     2161 agtactacaa agagcacggt tatgaaaccg tggttatggg cgcaagcttc cgtaacatcg
     2221 gcgaaattct ggaacttgca ggctgcgacc gtcttaccat cgcacctaca ctgctgaaag
     2281 agctggctga gagcgaaggg gctatcgaac gtaaactgtc ttacaccggc gaagtgaaag
     2341 cgcgtccggc gcgtatcact gagtccgagt tcctgtggca gcacaaccag gatccaatgg
     2401 cagtagataa actggcggaa ggtatccgta agtttgctat tgaccaggaa aaactggaaa
     2461 aaatgatcgg cgatctgctg taatcattct tagcgtgacc gggaagtcgg tcacgctacc
     2521 tcttctgaag cctgtctgtc actcccttcg cagtgtatca ttctgtttaa cgagactgtt
     2581 taaacggaaa aatcttgatg aatactttac gtattggctt agtttccatc tctgatcgcg
     2641 catccagcgg cgtttatcag gataaaggca tccctgcgct ggaagaatgg ctgacatcgg
     2701 tgctaaccac gccgtttgaa ctggaaaccc gcttaatccc cgatgagcag gcgatcatcg
     2761 agcaaacgtt gtgtgagcta gtggatgaaa tgagttgcca tctggtgctc accacgggcg
     2821 gaactggccc ggcgcgtcgt gacgtaacgc ccgatgcgac gctggcagta gcggaccgcg
     2881 agatgcctgg ctttggtgaa cagatgcgcc agataagcct gcattttgta ccaactgcga
     2941 tcctttcgcg tcaggttggt gtgattcgca aacaggcgct gatccttaac ttacccggtc
     3001 agccgaagtc tattaaagag acgctggaag gtgtgaagga cgctgcgggt aacgttgtgg
     3061 tacacggtat ttttgccagc gtaccgtact gcattcagtt gctggaaggg ccatacgttg
     3121 aaacggcacc agaagtggtt gctgcattca gaccgaagag tgcaagacgc gacgttagcg
     3181 aataaaaaaa tccccccgag cggggggatc tcaaaacaat tagtgggatt caccaatcgg
     3241 cagaacggtg cgaccaaact gctcgttcag tacttcaccc atcgccagat agattgcact
     3301 ggcaccacag atcagaccaa tccagccggc aaagtggatg attgcggcgt taccggcaat
     3361 gttaccgatc gccagcaggg caaacagcac agtcaggcta aagaaaacga attgcagaac
     3421 gcgtgcgcct ttcagcgtgc cgaagaacat aaacagcgta aatacgcccc acagacccag
     3481 gtagacacca aggaactgtg catttggcgc atcggtcaga cccagtttcg gcatcagcag
     3541 aatcgcaacc agcgtcagcc agaaagaacc gtaagaggtg aatgcggtta aaccgaaagt
     3601 gttgcctttt ttgtactcca gcagaccagc aaaaatttgc gcgatgccgc tgtagaaaat
     3661 gcccatggca agaataatac cgtccagagc gaaataaccc acgttgtgca ggttaagcag
     3721 aatggtggtc atgccgaagc ccatcaggcc cagcggtgcc ggattagcca acttagtgtt
     3781 gcccataatt cctcaaaaat catcatcgaa tgaatggtga aataatttcc ctgaataact
     3841 gtagtgtttt cagggcgcgg cataataatc agccagtggg gcagtgtcta cgatcttttg
     3901 aggggaaaat gaaaattttc cccggtttcc ggtatcagac ctgagtggcg ctaaccatcc
     3961 ggcgcaggca ggcgatttgc agtacggctg gaatcgtcac gcgataggcg ctgccgctga
     4021 ccgctttaac cccatttagt gccgcaccta cagggcctcc cagccccgcg ccgcgcagca
     4081 aaccatgccc aagtacgctc attgctgcgt gggtgcgtaa aatgcgggtc agttggctgg
     4141 aaagcaaatg cgacacgcct tttgccaata atttgtcttt catcagcagc ggcagcagct
     4201 cttccagctc attcaccctc gcatcgaacg cgtgcagaaa ctcctgctta tgttcctcgt
     4261 ccattttctt ccaggtatta cgcagaaatt gttccagtaa ctgttgctca atttcaaacg
     4321 tagacatctc tttgtcggct ttcagcttca atcgttttga aacatcgagc aaaatggccc
     4381 gatacaattt accgtgtccg cgcagtttgt tggcgatact atcgccacca aaatgctgta
     4441 attctccagc aatcagctgc cagttgcggc gatgttgctc gggatgccct tccatcgatt
     4501 taaacagttc gttgcgcatc agtacgctgg agaggcgagt tttgcctttt tcattatggg
     4561 tgagcaatcg ggcgaaattt gccaactgtt cctcactaca atgctgaaga aaatccagat
     4621 ctgaatcatt caggtaatta acattcattt tttgtggctt ctatattctg gcgttagtcg
     4681 tcgccgataa ttttcagcgt ggccatatcc gatgagttca ccgtatgacc ggaaaaggtg
     4741 atttttgaga cgcagcgttt attgtcgtta tcactgttaa tgttgatcca gtcagtggtt
     4801 tgcccttctt ttatttctga aggaatattc agactctgac tggcgctacg ggcg
//
LOCUS       DAZPEB010000229         4792 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZPEB010000229 DAZPEB010000000
VERSION     DAZPEB010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4792
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.234"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..544
                     /locus_tag="ACHURJ_003765"
     CDS             <1..544
                     /locus_tag="ACHURJ_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1282533.1"
                     /translation="GIVYISSQLVLSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQK
                     SLYLSGAGFCVLFAFPFFLLLDSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGT
                     KVRYTGLSFAYQFSAILGGLSPLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIE
                     PTDEQETASYRYIREQSHEN"
     gene            534..1421
                     /locus_tag="ACHURJ_003766"
     CDS             534..1421
                     /locus_tag="ACHURJ_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFR1282534.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRISPQAGPWD
                     DCFGFYNGVKVKLLWPGKLAMTMISSANSLVVFDKQPDATCINPLTQAPNAINLTPEL
                     VTPDRPLVIETRWQFTPES"
     gene            1562..2161
                     /gene="yihX"
                     /locus_tag="ACHURJ_003767"
     CDS             1562..2161
                     /gene="yihX"
                     /locus_tag="ACHURJ_003767"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFR1282535.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRVPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            2155..3028
                     /locus_tag="ACHURJ_003768"
                     /pseudo
     CDS             2155..3028
                     /locus_tag="ACHURJ_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
     gene            3025..3462
                     /gene="dtd"
                     /locus_tag="ACHURJ_003769"
     CDS             3025..3462
                     /gene="dtd"
                     /locus_tag="ACHURJ_003769"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFR1282536.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            3507..4448
                     /gene="fabY"
                     /locus_tag="ACHURJ_003770"
                     /gene_synonym="yiiD"
     CDS             3507..4448
                     /gene="fabY"
                     /locus_tag="ACHURJ_003770"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFR1282537.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
BASE COUNT         1120 a         1160 c         1250 g         1262 t
ORIGIN      
        1 tggtattgtc tatatttcca gccaattagt attgtcgcga gatattcccc tgacgggtat
       61 gttgattgcc tcggcgatcg gtattttcag ttgtccattg gttggatggt tatcagatcg
      121 tataggtcaa aaaagtctgt atttgtcggg agcaggattt tgtgtcctgt tcgcgtttcc
      181 tttcttctta ttactggata gcaaaagtac gctcatcatc tggtgcagta tgatcctcgg
      241 ctacaactta ggtccgacga tgatgtttgc cgtgcagcca acacttttca cccgcatgtt
      301 cggtaccaaa gtacgatata ccggcctttc ttttgcttat caattttccg cgattcttgg
      361 cggactaagc ccccttatcg cctcaagttt gctggcgcta gggggaggaa aaccgtggta
      421 tgtcgctctg tttttatttg ctgtctcagt tctttccttt gtttgcgtct ggctgattga
      481 gccgaccgat gaacaagaaa ccgcttctta ccgctacatc agggaacaat ctcatgaaaa
      541 ctgaacgaaa aaacgagcat tacctggcgc tacaacaggc gtttgacgcg ccatggcctg
      601 ggcctgtagg agagttagtg acgctggaaa aaggcaatat tcacctgcag atttatccgc
      661 atgatggtgc caggataacc tcgttaaagg catttggttc tgaagtatta cgccaatggc
      721 agccgcagcg cagagctttt cagtatggct gtttcccaat ggtgccgtgg gcagggcgac
      781 tggggaatgc gacgctgaat gctggtgggc aatgttattc tttacccgcc aataaaccac
      841 cacatgcgtt gcatggtatg gcttgttact caacgtggga aatcatcgac aaaacagcag
      901 attccctgac tttacgtatg cccctggctt caccgtggcc ctggcaaggg gaagtgattc
      961 agactttttt gctggagaac gatgcgctgg ttttgcaact ggaagtccat tcatatgccg
     1021 atacattccc ggcctccgct ggctggcatc cgtggtttgc gaaaaagctg actccgcaaa
     1081 atacagaatc gttgcaggtg ctttttgatg cggactggca agaagaagcg ggtagtgatg
     1141 aactgccaac tggaaatcgt atctctcctc aagccggtcc ctgggatgac tgttttggtt
     1201 tttataacgg ggtgaaggtt aagttactgt ggcctggtaa actcgcgatg accatgatct
     1261 cttctgcaaa cagtctggtg gtatttgata aacagcccga tgccacctgt attaacccgc
     1321 tgactcaggc acctaacgca ataaatctaa cgccggaatt ggtgacacca gataggccgc
     1381 tggtcatcga aactcgttgg caatttacac ctgaatctta accattcatc tattgcgaaa
     1441 aagccagtga aattactctg ctttttcgca atcagcatca caagattacc cttattgcca
     1501 catagccaaa cccatccttt cccgctacag ttaatttctt gtggcgcgaa aggaggcaaa
     1561 aatgctctat atctttgatt taggtaatgt gattgtcgat atcgacttta accgtgtgct
     1621 gggagcctgg agcgatttaa cgcgtgttcc actggctacg cttaagaaga gttttcatat
     1681 gggggaggcg tttcatcagc atgagcgtgg ggaaattagc gacgaagcgt tcgcagaggc
     1741 gctgtgtcat gagatggctc taccgctaag ctacgagcag ttctctcacg gctggcaggc
     1801 ggtgtttgtt gcgctgcgcc cggaagtgat cgccatcatg cataaactgc gtgagcaggg
     1861 gcatcgcgtg gtggtgcttt ccaataccaa ccgcctgcat accaccttct ggccggaaga
     1921 atacccggaa attcgtgatg ctgctgacca tatctatctg tcgcaagatc tggggatgcg
     1981 caaacctgaa gcacgaattt accagcatgt tttgcaggcg gaaggttttt cacccagcga
     2041 tacggtcttt ttcgacgata acgccgataa tatagaagga gccaatcagc tgggcattac
     2101 cagtattctg gtgaaagata aaaccaccat cccggactat ttcgcgaagg tgttatgcta
     2161 aaaaccattc aggacaaagc caggcaccgt acccgtccac tatgggcctg gctaaaacta
     2221 ctctggcaac gtattgatga ggacaacatg acaaccctgg caggtaacct tgcctatgtg
     2281 gtcgttgctc tcattagtgc cgctggttgc cgttgttttt gcgcttttcg ccgcttttcc
     2341 catgttttcc gacgtcagca ttcagttacg tcactttatt tttgccaact ttctgcctgc
     2401 tactggcgat gttattcagc ggtatatcga acaatttgtt gccaattcca acaagatgac
     2461 cgccgttggg gcgtgcgggc tgatcgtcac cgcgttattg ttgatgtact ccatcgatag
     2521 cgcgttgaat accatctggc gcagtaaacg agcgcgacca aaaatttact cgttcgccgt
     2581 gtactggatg attttaacgc tggggccgct gctggcaggg gccagtctgg cgatcagttc
     2641 ctatttgctc tccctgcgct gggcgagcga tctcaatact gtcatcgaca acgtgctgcg
     2701 tatttttccg ttgctgttgt cgtggatctc cttctggttg ctgtacagca ttgttcctac
     2761 catccgcgta cctaaccgcg acgcgattgt cggcgcgttt gtcgccgcac tcctgttcga
     2821 agcaggaaag aaaggtttcg cgctttatat caccatgttc ccgtcatatc agctcattta
     2881 cggtgtgctg gcggtgatcc ccattctctt tgtttgggtc tactggacat ggtgtatcgt
     2941 cttgcttggc gcggaaatta ctgtcactct cggggaatac cgcaaactaa aacaagcagc
     3001 tgaacaagaa gaagacgacg aaccatgatt gcattaattc aacgcgtaac ccgtgccagc
     3061 gtcaccgtgg agggagaagt gacgggcgaa attggcgcgg gacttttggt gttattgggt
     3121 gtcgaaaagg atgacgacga acagaaagca aaccgtctgt gcgagcgtgt gctcggctac
     3181 cgcatcttta gcgatgccga aggcaagatg aatctcaacg tgcaacaggc gggcggcagt
     3241 gtactggtgg tttcccagtt taccctcgcc gcagataccg aacgggggat gcgcccaagt
     3301 ttctccaaag gcgcatcacc ggatcgcgca gaggcgttat atgactattt cgtcgaacgc
     3361 tgccgccagc aagagatgaa cacgcaaaca ggacgcttcg ctgccgatat gcaggtatcg
     3421 ctggtcaatg atggccccgt gacattctgg ttgcaggtat gagccagctt ccagggttgt
     3481 cacgggaaac aagagagagt atcgctatgt atcaccttcg ggttccacaa acagaagaag
     3541 agttagagcg ttactatcag tttcgctggg aaatgttgcg taagcccctg catcaaccaa
     3601 aaggttcgga acgcgacgcg tgggatgcga tggcgcatca tcagatggtc gtcgacgagc
     3661 aggggaatct ggtggcggta ggccgactgt atattaatgc cgacaatgaa gcgtccattc
     3721 gctttatggc cgttcatccc gacgtgcagg acaaagggct ggggacgttg atggcgatga
     3781 ccctggagtc ggtggcgcgt caggaaggcg ttaagcgcgt gacctgtagc gcccgtgaag
     3841 acgcggtgga gtttttcgcc aagctggggt ttgttaatca gggagaaatc accacaccaa
     3901 ccaccacgcc gattcgccat tttttgatga ttaaacctgt tgccactctg gatgatattt
     3961 tgcatcgcgg cgactggtgc gcgcagctgc aacaggcgtg gtacgaacac atcccgctta
     4021 gtgaaaaaat gggcgtgcgc attcagcaat ataccgggca aaaatttatc accaccatgc
     4081 cggaaaccgg taatcagaat ccgcaccata cgctgtttgc cgggagttta ttctcgctgg
     4141 caacgctcac cggttggggg cttatctggc tgatgctgcg cgaacgccac ctcggcggaa
     4201 cgattattct ggccgatgcg catatccgct acagcaagcc gattagcggt aaacctcatg
     4261 cggtagccga ccttggtgcc ttaagcggcg atctcgaccg tctggcgcgc ggacgaaaag
     4321 cacgggtgca gatgcaagtt gaaatctttg gcgacgagac gccgggtgca gtgtttgaag
     4381 gcacgtatat cgttctgccc gcgaagccat ttggcccgta tgaagagggc gggaacgaag
     4441 aagagtagct tatggagagg ggcggtgctg cctctctcat tcattccggt catttacatc
     4501 attccctgtc tatgccggta atattgaata agaactctat aaatttttat tcttcgttgg
     4561 caccatacgt gtcttgctac atcactgttc aggagcattt aaatattatt tgtagagctg
     4621 agtggcaatt atcgcagata atgcttaact catcatctta gttcaggttt gagcgatttt
     4681 tgatagtcaa aacctcctct ggtgccatac tttcctcata aatattttta aaatctatag
     4741 ctcacaaaca ttatgcaaag ataatgggct ggcatcattg tcagtatcat tc
//
LOCUS       DAZPEB010000230         4772 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZPEB010000230 DAZPEB010000000
VERSION     DAZPEB010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4772
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.235"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(248..323)
                     /locus_tag="ACHURJ_003771"
     tRNA            complement(248..323)
                     /locus_tag="ACHURJ_003771"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(288..290),aa:Asn,seq:gtt)
     gene            complement(424..1911)
                     /gene="yeeO"
                     /locus_tag="ACHURJ_003772"
     CDS             complement(424..1911)
                     /gene="yeeO"
                     /locus_tag="ACHURJ_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFR1282538.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKVLA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSW
                     PGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIG
                     IPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITG
                     RRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILI
                     WLNALFMPIWSASWGLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVG
                     VWMGMFADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPVVSE"
     gene            2072..2147
                     /locus_tag="ACHURJ_003773"
     tRNA            2072..2147
                     /locus_tag="ACHURJ_003773"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2105..2107,aa:Asn,seq:gtt)
     gene            2309..3571
                     /locus_tag="ACHURJ_003774"
     CDS             2309..3571
                     /locus_tag="ACHURJ_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1282539.1"
                     /translation="MSLNDSKIRNLKPSSKPVKLSDSHGLYLLVNQGGSRIWYLKYRF
                     SGKESRVSLGAYPLVSLAEARQRRDDIRKLLTQNINPAHQRMSDKAAASPEKYFKAVA
                     LAWHKTNKKWSPDYAARILASMENHIFPAIGHLPVTTLKTQNFTALLRVIEDKGFLEV
                     ASRTRQQLCNIMRYAVQQGFTENNLAQHLEGVTAPPVKNHYPALPLERLPELFERIGD
                     YQQGRQLTRLAVVLTLHLFIRSSELRFARWGEIDFRNKIWTIPATREAIEKVRFSGRG
                     AKMRTPYIVPLSRQAIAILKQIKEISGHLELVFPGDHNPYKPMSENTVNRALRLMGYD
                     TKTDVCGHGFRTMACSALVETELWSRDTVERQMSHQERNSVRAAYIHRAEHLDARTAM
                     MQWWSDYLDVSREGYVAPYIYARRHKAA"
     gene            complement(4290..>4772)
                     /locus_tag="ACHURJ_003775"
     CDS             complement(4290..>4772)
                     /locus_tag="ACHURJ_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HFR1282540.1"
                     /translation="MAAALELSRLMGLRSQEVVQSAQSLRTWKQSLERGEPRLTVVFG
                     TKGGRPRETVILDAVAIRKALDNALVVAEDRHGRLIDKPDLKSAMKYWHSQASRLGLT
                     GTYSPHSLRYAWAQDAIRHYLAQGFSEKEALAMTAMDLGHGDGRGRYVAQVYGRKDTD
                     "
BASE COUNT         1318 a         1251 c         1064 g         1139 t
ORIGIN      
        1 tacatcccgc ataaggcatt tttctcttta tccttgtacg gtacttcatg gaacagagtt
       61 tttgaccttg cgaatcgtga tgtctgttgg ggaggggcaa tttgctcact gaagcgtgag
      121 actcgattaa gcgcacgaaa cacagaaatc aaaaaacccg ggcacttttt tacaaggtaa
      181 ccgggtaaaa ataattttta ttttttaact gttttgagac tcatagagat gtctcaaaac
      241 taaaatttgg ctcctctgac tggacttgaa ccagtgacat acggattaac agtccgccgt
      301 tctaccgact gaactacaga ggaatcgtga gaacgaggcg aatattagcg atgcccaccc
      361 acaatgtcaa agcctgttat ttaaatttga aatcgtttgc tgaaataatc tgcattttgt
      421 cgtttattcc gacacaactg gctttttttc acacttttgc ggttcggatc gagggtattt
      481 ccacagccaa cgtccagtaa ccattcgcca gtaaaacagc acggcccgca cagcccagtc
      541 ggcaaacatt cccatccaga caccaaccac accccagcca agcatgatgc ccagcacata
      601 accgacgaca acccggcaac cccacatgct caacatagaa acccacatgg cgtaacgggc
      661 atcgcgagca cctttaaatc cagcgggtag cccccatgag gcggaccaaa taggcataaa
      721 taaagcattt agccaaatca gaatcacaac gacatgttta acctgtggat cctgggtgta
      781 aaacgatgcc ataaccccgg caaagggagc cgttagccag gcgatggccg ttaatccaag
      841 agtggaaagc cagaacacat gccgcaactg aatctctgct tgcgctatct gccctacccc
      901 caaccttcgg cctgtaatga tcgtagaagc agagccgagc gcacttccgg gtaagttgat
      961 aagagccgca attgaaaacg cgataaaatt tccggcaata acactggtcc ccatcccggc
     1021 aacgaacatt tgggttaata accgaccact ggtaaataac actgattcga cactcgcggg
     1081 aataccaatc cccatgactt cccagataat gctaaaattc agcggtttaa aatagctctt
     1141 taacgaaatc cttagcgcag gattaaaacc aatcgccagc acccacaaaa ttgcaactgc
     1201 gccaatataa cgagaaatgg ttaaacccag ccctgccccg acaaatccca gtcccggcca
     1261 ggagaaaagg ccgtaaatca atatgccgct aataataata ttaagaatat tcaggctacc
     1321 gttaatcaat agcggtattt tcgtattccc tgcaccacga agtgccccgc taccaataag
     1381 agtgatggca gctgctggat aactgagtac cgtcagctcc agataagtca acgccagtac
     1441 tttaacttct gtcgtggcat cacccgcgac gaaatcaata atttgttcgc caaaatgatg
     1501 aataagcgtt gccaacagta cggcaaacaa cgtcatgatc accaatgact gccgcgtcgc
     1561 caccctcgct cgtcgtcgat cccgcttacc gagactaaat gccacaacga cagtagtacc
     1621 aagatcgata gcagcaaaaa aagccataat gaccatattg aagctgtccg ccaatcccac
     1681 gccggccatc gcatcttttc ccagccagct gaccagaaaa gtgctcagaa cccccatcaa
     1741 caggacacag gcattctcca tgaagatagg aacagcaagc ggggttatct cgcgccagaa
     1801 caacactttg tagctcttgc gtttagcgtg ccagcgagtg ccgtgaacaa cctggcgtaa
     1861 agcagaggag atattcaaag ccgaccttaa ttgcagaaag tgaaaccaca tttcaaataa
     1921 tgagggagaa tcagcaaagc tgcaaagatt ttcgccaaca aattgtctgc aaatgcaaca
     1981 aactgttgat agaaacggca aacagttggg gaatttaaaa atcgggtttg acaaaagatt
     2041 tttcgccgtt aagatgtgcc tcaacagcga ttcctctgta gttcagtcgg tagaacggcg
     2101 gactgttaat ccgtatgtca ctggttcgag tccagtcaga ggagccaaat tccgattctt
     2161 acgctatctt attagtcctt atttgatatt gattcaataa gttagcgtga aaatctttcc
     2221 agatgtgtat tcagttttcc ctccgcatcc ggttaatttg gtggtcagat ttgggggcag
     2281 gttggttcga ttctggagtg acccccaaat gtctttaaac gattcaaaaa tccgtaactt
     2341 aaaaccctct tcaaaacccg taaaactgtc tgattcgcac ggtctgtatc tgctggtcaa
     2401 tcagggcggt tcgcgtatct ggtatctcaa gtatcgcttc agtggtaaag agtcccgcgt
     2461 cagtctgggc gcatatcctc tggtttcact tgctgaagca agacaacgac gtgacgatat
     2521 ccgtaagcta ctgacgcaga atattaaccc cgcccaccag cgcatgtctg ataaagccgc
     2581 agcgtcaccg gaaaagtatt ttaaagccgt ggcgcttgca tggcacaaaa ccaacaaaaa
     2641 atggtcgcct gattatgccg cgcgcatcct cgccagcatg gaaaatcata tctttccggc
     2701 gattggtcat ctgcctgtca ctacgctgaa aacgcagaat ttcactgcgt tactgcgggt
     2761 tattgaggat aaaggttttc tggaagtcgc gtcccgaacc cggcagcaac tctgcaacat
     2821 catgcgttac gccgtacagc agggattcac cgaaaacaac ctggcgcagc atctggaagg
     2881 cgtcaccgcg ccgccggtta agaatcacta tcccgccctg ccgctggagc ggttgcccga
     2941 attgtttgag cgcattggcg actatcagca gggacggcag cttacgaggc tggcggtggt
     3001 gttgactctc cacctgttta tccgttccag cgagctgcgc tttgcccgct ggggtgagat
     3061 agatttcaga aacaaaatct ggaccatccc cgccacaagg gaagcgattg agaaagtccg
     3121 tttctctggc cgtggcgcaa aaatgcgtac tccatatata gtgccgcttt ctcgtcaggc
     3181 tatcgccatt ctgaagcaga taaaggaaat ctccggccat ctggaactgg tgttccccgg
     3241 cgaccataac ccgtataagc cgatgagcga aaacactgtc aaccgggcgt tgcgtcttat
     3301 gggctatgat accaaaactg atgtctgtgg gcatggattc cggacaatgg cgtgtagcgc
     3361 attagtggaa actgaactct ggtcacggga taccgtggag cggcagatga gtcatcagga
     3421 gcgcaacagc gttcgggcag cgtatatcca ccgggctgaa catctcgacg cccgtacagc
     3481 catgatgcag tggtggtcgg attatctgga tgtaagtcgc gaggggtacg ttgcgccgta
     3541 tatttatgcg cggcggcata aggcggcctg agacagtggc tatataattt gttaaaaatg
     3601 aaaaagcaga tcctgattga atccggatct gctttttaca ttaggcaatc gctgccagac
     3661 aatctgcacc agaaacgcca taactgcacc agttcagtac caccgcttac ctgcgccagt
     3721 ctgccgtcgc ctgtctgtgt caggactgcg ccagatctta tcgaataaac atcaaactgc
     3781 gccagtgtta cggccctttc agccctttgg ccttttccgg tctggcgctg ttacccttag
     3841 ccgcccctta ccctttccgg cctttttcct ttgctggcgc aatcgccaga aaaatcgtca
     3901 agctgcacca gaagcctgcc aataggtcat accgttcaga agcatcgtta ttgagaggaa
     3961 accgtacaaa aaatttctac gtagaaattt tttcggtgga taacgatgaa taaagagtat
     4021 tttataatgt gatggctatc aaaaatcgac catccaaaaa taattcattg ttcattgatt
     4081 tataatgaat aaccatgaat ttaatagtcg atgattattg tttttatcag tattgatagg
     4141 aaagtataca aaaaattttg gccccaaaat tttttgtacg aaaaccgatg aataacgcga
     4201 aattaatagg aaaccatcca aaattatttg gggcaaataa ttttgcccga aaaccgatgg
     4261 ataactcgat cttttcaaag caacggctat caatcagtat cctttcttcc gtaaacctgc
     4321 gccacatacc gcccgcgccc gtcgccgtgt cccaaatcca tcgccgtcat tgccagcgct
     4381 tctttctcac tgaatccctg cgccagataa tgacgaatgg catcctgcgc ccacgcatag
     4441 cgcaacgagt gcggggaata tgtgccagtt aacccgagac gtgacgcctg gctgtgccag
     4501 tatttcatcg cgcttttcag gtcgggttta tctatcagcc tgccgtggcg atcttctgcc
     4561 acaaccaacg cattatccag cgcttttctg atagcgacag catccagaat aacggtttct
     4621 cgcggacgcc cgcctttggt gccgaacacc acggttaaac ggggctcacc ccgctccagc
     4681 gattgcttcc acgttcttaa tgactgcgca ctttgcacca cctcctgcga acgtaatccc
     4741 atcagccttg agagttccag cgccgctgcc at
//
LOCUS       DAZPEB010000231         4712 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZPEB010000231 DAZPEB010000000
VERSION     DAZPEB010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4712
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.236"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(86..2365)
                     /gene="maeB"
                     /locus_tag="ACHURJ_003776"
     CDS             complement(86..2365)
                     /gene="maeB"
                     /locus_tag="ACHURJ_003776"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFR1282541.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            2654..3604
                     /gene="tal"
                     /locus_tag="ACHURJ_003777"
     CDS             2654..3604
                     /gene="tal"
                     /locus_tag="ACHURJ_003777"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFR1282542.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGISAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMAPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            3624..>4712
                     /locus_tag="ACHURJ_003778"
     CDS             3624..>4712
                     /locus_tag="ACHURJ_003778"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016744 - transketolase or transaldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HFR1282543.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTGYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNT"
BASE COUNT         1162 a         1257 c         1199 g         1094 t
ORIGIN      
        1 aaactcagta ataagcttta ctaatatatt gcagccaaac cgcttaccct gaatattcag
       61 ggtaagcgtg agagttaaaa aagaattaca gcggttgggt ttgcgcttca accacggcca
      121 gcgccaccat gttgacgata cgacgcaccg atgcgatcgg cgttaacacg tgaaccggtt
      181 tcgccacgcc catcagcacc ggaccgacag tcacaccttc cgagctggaa acgcgcagta
      241 agttgtaact aatgcgggca gcttccatgt tcggcatcac cagaatattg gcagaacctt
      301 tcaaagggct gtccggcata cggtcgttgc gaatcgcttc caccagcgct gcatcgccgt
      361 gcatttcacc atcaatcatc agatctggcg cacgatccct gaccagttcc agcgcctgac
      421 gcattttgct cgacgacggg cagtcagaag aaccaaagtt ggagtgcgac aacagagcaa
      481 cgcgcggctc aataccaaaa cgacggacag tttctgccgc catcaaggtg atctccgcca
      541 gctcttctgc gtctggttcg tcattgacgt aggtatcggc aataaaggtg ttaccactcg
      601 gcagcagcag cgcgttcatt gcacctgcgg tgtgaacgcc atcgcgataa ccaaagacat
      661 ttttcaccac gctaaaatgt tcatgataat cgcccaccgt accgcagatc attgcatcgg
      721 cttccccacg ctgaaccatg atcgcgccga tcactgtcgg gttactgatc agcgcccgct
      781 gcgcctgttc ctgagtgaca ccgcgacgct tcatgatctg gaagtattcg gtccagtact
      841 ctttaaagcg cggatcggat tcgttattga cgatctcaaa atcaacgccc gctttgatct
      901 gcaagcccag tttctgaatg cgcatttcga tcacgttcgg acgaccgata aggatcggtt
      961 tcgccagtcc cagcgttacc agttcctgag tggcatgcag aacgcgcgcc tcttcccctt
     1021 ctggcagaac aacgcgcttc ggcgctttgc gagcctggga gaaaatcggc ttcataaaca
     1081 ggttggtttt gtaaacgaac tcagtcagct tgtcgatgta gacgtcgaaa tcagcaatcg
     1141 gacgcgtcgc cacgcccgac tccatcgcgg ctttagcgac cgcaggagcg atcttaacga
     1201 tcaagcgcgg atcaaacggt tttggaatga tgtattccgg accaaagctc agatcctgat
     1261 cgccatacgc tgaagccacc acttcgctct gttccgcatg ggcgagttct gcaatcgcac
     1321 gtaccgccgc cagtttcatc tcttcgttga tggcggttgc gccaacgtcc agcgcgccac
     1381 ggaagatgaa cgggaagcac aggacgttgt tcacctggtt cggatagtca gaacgaccgg
     1441 tgcaaatgat ggcatccgga cgcacttctt tcgccagcgg cggcagaatt tccggttccg
     1501 ggttcgccag cgccaggatc attggcgcac gagccatttt cttcaccatt tcctgggtca
     1561 gcactttcgg gccggaacat ccgaggaaaa tatccgcgcc ttcaatcaca tcatcgaggg
     1621 tacgtttgcc gtcgtccacc accgcatatg cggctttggt ttccgccatg tttggctcac
     1681 ggccctgata gataacgcct tttgaatcgc aaaccacgat gttatgtttt tgcagaccca
     1741 gcgctaccag caggttcata caggcgattg ctgcggcacc cgcgccggaa accaccatcc
     1801 gcacgtcgga gatgtttttc tccaccacgc gcaagccgtt gaggatggcg gcagtgctga
     1861 tgattgccgt gccgtgctga tcgtcgtgga ataccggaat attcatccgc tcgcgcagtt
     1921 tctgttcaat atagaaacat tctggcgctt taatgtcttc gaggttgatt ccgccgaagg
     1981 tcggttcgag cgcagcgaca acttcaataa atttgtccgg gtcgagttca tcaacttcaa
     2041 tgtcaaatac atcaatcccg gcgaatttct taaacagaac gcccttgcct tccatcaccg
     2101 gtttgccagc cagcgcgcca atgttgccta accccagcac cgccgtaccg ttagagatca
     2161 ccgccaccag gttaccgcgc gcggtatatt tgtaggcttt taacgggtct ttttcgattt
     2221 caagacaagg tgcggcaacg cctggtgagt aggccagcgc cagatcgcgc tgcgttgcca
     2281 gaggcttggt tggagaaacc tggattttcc ctggaactgg aaattcatgg aaatcaagtg
     2341 cactttgttt taactggtca tccatttggt tgttcctttc acgtaacgtt cacaaataaa
     2401 gtgtgtgggc aacagcccct gcccacaacg tggcgcacat tattaccccg ccggagtcta
     2461 cagactttga gcaagtccaa actctcacca ttaatataat gttttggtaa taatcctata
     2521 acactgatgt tacctgctta atccagcaat accatgcctg tctgctatgc ttttttgatg
     2581 cgtttagcga aatttctcag aagtgtgaat taacgcactc atctaacact ttacttttca
     2641 aggagtattt cctatgaacg agttagacgg catcaaacag ttcaccactg tcgtggcaga
     2701 cagcggcgat attgagtcca ttcgccatta tcatccccag gatgccacca ccaatccttc
     2761 gctgttgctc aaggctgccg gattatcaca atatgagcat ttaatagacg atgctatcgc
     2821 ctggggtaaa aaaaatggca agacccagga acaacaggtg gtcgcagcgt gtgacaaact
     2881 ggcggtcaat ttcggtgctg aaattctcaa aatcgtaccc ggtcgcgtgt caacagaagt
     2941 tgatgcacgc ctctcttttg ataaagaaaa gagtattgag aaggcgcgcc atctggtgga
     3001 cttgtatcag caacaaggcg ttgagaaatc acgcattctg atcaaactgg cttcgacctg
     3061 ggaaggaatt agcgcggcag aagagctgga aaaagaaggt attaactgca acctgacgct
     3121 gctgttttct tttgcccagg cgcgggcctg tgcggaagca ggcgtttttc tgatttcgcc
     3181 gtttgtcggg cgtatttatg actggtatca ggcacgcaag ccgatggccc cgtatgtggt
     3241 ggaagaagat ccgggcgtta aatcggtgcg caatatctac gactactata agcaacacca
     3301 ctatgaaacc attgtgatgg gcgcgagctt ccgtcgcacc gaacaaatcc tcgccttaac
     3361 cggctgcgat cgactgacta tcgcaccgaa tttgctgaag gagctgcagg aaaaagtttc
     3421 gccagtggta cgtaaattaa tcccaccttc tcagacgttc ccacgcccag ctcccatgag
     3481 cgaagcggag ttccgttggg agcacaatca ggatgcgatg gcggtagaaa aactgtctga
     3541 aggcattcgt ctgttcgccg ttgatcaacg caaactggaa gatcttcttg ccgccaaact
     3601 ataaaccagc cacggagtgt tatatgtccc gaaaagacct tgccaatgcg attcgcgcac
     3661 tcagtatgga tgcggtacaa aaagccaact ctggtcatcc cggcgcgccg atgggcatgg
     3721 ctgatattgc cgaagtgctg tggaacgatt ttcttaaaca taaccctacc gacccaaccg
     3781 ggtatgatcg cgaccgcttt attctttcca acggtcacgc gtcgatgctg ctctacagtt
     3841 tgctgcatct gaccggttac gacctgccgc tggaagagct gaagaacttc cgtcagttgc
     3901 attcgaaaac cccaggccac ccggagattg gttatacgcc cggtgttgaa accaccaccg
     3961 gtccacttgg acaaggtttg gcgaacgccg tcggactggc gatagcagag cgtacactgg
     4021 cggcgcagtt taatcagccg gatcatgaaa ttgtcgatca cttcacctat gtgtttatgg
     4081 gcgacggctg cctgatggaa ggtatttccc acgaagtctg ttcgctggcg ggcacgctag
     4141 gactgggcaa gctgattggt ttttacgatc acaacggtat ttcgattgat ggagaaaccg
     4201 aaggctggtt taccgacgat acggcaaaac gttttgaagc ctatcactgg catgtgatcc
     4261 atgaaatcga cggtcacgat ccgcaggcgg tgaaggaagc gatccttgaa gcgcaaagcg
     4321 tgaaagataa gccgtcgctg attatctgcc gtacggtgat tggctttggt tcgccgaata
     4381 aagcaggtaa ggaagaggcg cacggcgcac cactggggga agaagaagtg gcgctggcac
     4441 ggcaaaaact gggctggcac catccgccat ttgagatccc taaagagatt tatcacgcct
     4501 gggatgcccg tgaaaaaggc gaaaaagcgc agcagagctg gaatgagaag tttgccgcct
     4561 ataaaaaggc tcatccgcaa ctggcagaag agtttacccg acggatgagc ggtggtttac
     4621 cgaaggactg ggagaaaacg actcagaaat atatcaatga gttacaggca aatccggcga
     4681 aaatcgctac ccgtaaggct tcgcaaaata cg
//
LOCUS       DAZPEB010000232         4699 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZPEB010000232 DAZPEB010000000
VERSION     DAZPEB010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4699
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.237"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..147
                     /locus_tag="ACHURJ_003779"
     CDS             <1..147
                     /locus_tag="ACHURJ_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFR1282544.1"
                     /translation="VKQFLSRDPFECVLCGGRMVYRRAIAGLNVEGLKKNARDISLLR
                     YMPA"
     gene            complement(303..1217)
                     /gene="nrdF"
                     /locus_tag="ACHURJ_003780"
     CDS             complement(303..1217)
                     /gene="nrdF"
                     /locus_tag="ACHURJ_003780"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFR1282545.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPYALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYSDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSRIGN"
     gene            complement(1227..3371)
                     /gene="nrdE"
                     /locus_tag="ACHURJ_003781"
     CDS             complement(1227..3371)
                     /gene="nrdE"
                     /locus_tag="ACHURJ_003781"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFR1282546.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAYAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPVAGRINMSNLCSEIL
                     QVNCASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(3344..3754)
                     /gene="nrdI"
                     /locus_tag="ACHURJ_003782"
     CDS             complement(3344..3754)
                     /gene="nrdI"
                     /locus_tag="ACHURJ_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFR1282547.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGMPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            complement(3751..3996)
                     /gene="nrdH"
                     /locus_tag="ACHURJ_003783"
     CDS             complement(3751..3996)
                     /gene="nrdH"
                     /locus_tag="ACHURJ_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFR1282548.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(4244..4573)
                     /locus_tag="ACHURJ_003784"
     CDS             complement(4244..4573)
                     /locus_tag="ACHURJ_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFR1282549.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
BASE COUNT         1147 a         1184 c         1224 g         1144 t
ORIGIN      
        1 gtgaaacagt tcctgagtcg tgacccgttc gaatgcgtgc tgtgtggcgg ccggatggta
       61 taccgccggg ccattgcggg gctgaatgtg gaggggctga agaaaaacgc gcgggatatc
      121 agtctgctga ggtatatgcc ggcctgacac aggctaagtg tgcccgggaa tcggtgcaag
      181 tataaaaaac agcctgttgc ggtataagaa tcgaccatgc agagagagaa aacaccggag
      241 tggagggaaa agcagaaaag ctcacgtggt ataaggcgcg gacaggggta tcgcttagtt
      301 tttcaatttc ctatacgtga accggaaaag aaatcgtgat tttcatcggc attcggcgaa
      361 agcgcggcga ggattgccgg attcacttcc gccatttctg cgggaaataa cggttcgtag
      421 cccagattca tcaaagcctt attggcgttg taacagagaa acgctttcac atcgtcagcc
      481 cacggggttt cggcgtacag ctcatcgctg tattgcaact cgttgtcgta gagttccagc
      541 aacaaatcga aggcgaaact cttcaactct tcacgttgtc ccagagatat cttttccatg
      601 tttttctgat atttatagcc tatgtagtaa ccgtggactg cttcatcgcg gataatcaga
      661 cggatcagat ctgcggtatt ggtcagcttt ccgcggctgg aaaaatacat cggcagccag
      721 aaaccggaat agaacaaaaa agattcaaga aacacactgg cgattttctt tttcagcgga
      781 tcatcaccgc gataatgttg ctgaataatc tgagcttttc gctgcaacgg tgcgttttct
      841 tcactccagg cgtaggcggc atcgacatct ttggtctgac acagcgtcga gaaaatcgaa
      901 ctgtaagagc gggcatgaac cgcttccata aagctgatat tcgataatac cgcttcttca
      961 tgaggcgtga gtgcataggg catcagcgaa ggcgcgccga taacattttg cagcgtgtcg
     1021 agcagcgtca ggccagtaaa aacgcgcatc gtcagttgtt gttctacgac agttaatgtc
     1081 tgccaggcag gaatatcgtt cgacagcggc accttttctg gtagccagaa attgctggtc
     1141 aggcgattcc acacctccag atctttatcg tcagatatct tgttccagtt gatggcgctg
     1201 atacgtgaga gtttcataga tattccttaa agtgcacagg agacgcagcc ttcaatttca
     1261 gtgccttcca gcgccatctg acgcaggcga atgtaataga gcgttttgat acccttgcgc
     1321 caggcgtaaa tctgcgcttt gttgatatcg cgagtggtgg cggtatcggg gaaaaacagc
     1381 gtcagcgaca gcccctgatc gacatggcga gtcgcttcgg cgtaggtgtc gatgatcttt
     1441 tctgcgccga tttcgtaagc atcctgatac agcgccagat tctcgttagt cataaacggg
     1501 gcagggtagt aaacgcgtcc tgttttgccc tctttgcgta tctctacttt cgccacaatc
     1561 ggatgaatac tcgacgtagc atggttgata taagagatag aaccggttgg cggcaccgcc
     1621 tgaagattct ggttataaat gccgtagcgc atcacgtcgt cgcgcagttg cgcccacatc
     1681 tcacgggtag gtaacgtaat accgctgcgg gcaaacagtt cgccaacttt cgccgttttc
     1741 ggctgccagt taccttgcag atattggcta aaatattcac cactggcata acgtgactgt
     1801 ttgaacccgg cgaaggtttc accgcgttcg cgcgccagca acatcgaggt acgcagtgcg
     1861 tgccaggtga tggcatagaa atagagattg gtgaaatcca gtgcttccgg cgaaccataa
     1921 gcgatgcctt ctcgcgccag atagccgtgt aaattcatct gccccagtcc gatggcgtgt
     1981 gaggcggcat ttccggcttc gatggacggc acgctgcgga tatgactcat atctgacacc
     2041 gccgttaaac cgcgcacggc agtctctacc gtgcgggcaa aatcggggga atccatggtg
     2101 tgcgcaatat tcaacgaacc taaattgcag gagatatcat ggcctgtgcg ggcatagtcg
     2161 agattctcgt catactctga ggcacagttg acctgcaaaa tctctgagca gagattactc
     2221 atatttatgc gcccggcgac agggttagca cggtttaccg tgtcttcata catgatgtag
     2281 ggatagccgg actcaaactg gatttctgcc agccgctgga agaaatcacg ggcgttgagg
     2341 tattttttgc ggatgcgttc atcggcaacc agttcgtcat agtgttcgct gatggcgata
     2401 tcggcaaacg gcttgccata aactcgctca acgtcatagg gcgaaaacag tgccatctgc
     2461 gcattctctt ttgccagatg gaaagtgata tccgggatca ccacgcccag cgacagtgtt
     2521 ttaatgcgga ttttttcgtc ggcattttcc cgttttgtat cgagaaaacg cagaatatcg
     2581 ggatgatgag catgtaaata gactgcacct gccccctgac gcgcgccgag ttggttggca
     2641 taggaaaatg cgtcttccag cattttcatc accggaatta cgccagaaga ttgattttca
     2701 atacgtttaa ttggcgcgcc cgcttctcgc agattcgaca gcaaaaatgc cacgccgccg
     2761 ccgcgtttcg acagttgcag tgcggaattt accgcccggc caatcgactc catattgtct
     2821 tcaatacgca gcagaaaaca ggaaaccagt tcgccgcgct gctgcttacc gcagttgagg
     2881 aatgtcggag tggctggctg aaagcgtcct gacagcattt catcggtcag ttgcaacgcc
     2941 agcgtcgcat cgccttgtgc cagcgtcagc gccaccatcg tgactcgatc ggcaaaatct
     3001 tccagataac gtttaccgtc gaatgtcttc aacgtatagc tggtgtaaaa cttccatgcc
     3061 ccgaggaatg tctggaaacg aaaaccgctg gtgtgtgcgt aggcaaacag cgtaatgaca
     3121 aagtcgcgag agtagcgatt aagaacgctt tcatcatagt aaccttcgtt gaccagccag
     3181 ttcaggcgct gctgctggct actgaaggtc acactgttcg gacgcacatg cgtcgccata
     3241 aaggcgtcaa cggcctggcg gtctttatcg aactgaatgc gacctgcgct atcgtagagg
     3301 ttaagcatcg cattcagcgc gtggtaatcc atcgtttcct gcgtcaggca ttctgcggtt
     3361 gtcgttgcca aaattcggtt actcctttac gaacgttttc gatatcgctt tgcgtaccca
     3421 tgagttcaaa acggtacagc cacggcacgc cgcatttccg ggcaatcaca tctccggcgc
     3481 ggccatacgc ctcaccaaag ttgcgattac cagaagcaat aacgccgcga agcaacgccc
     3541 ggttgtgctc gtcgtttaaa aagcgaatta cctgtcgtgg caccgcgcca gccgtaccgc
     3601 cgccgccgta agagggcacg atcaggatgt aaggctcgtc tacctgaatc cgttcccgct
     3661 cattgagcgg gatgcgcacc gcgggcatac ctaaacgttc gataaaacgc tgcgtgtttt
     3721 cggagctgct ggagaagtag acgagctggc tcatgcactg gccgcgtgtg gcgctggatg
     3781 cagacggtta atcatgtccg gacggaaacc agaccagcta agatcgccag caatcactac
     3841 cggcaactga cgaaagccct gagcacgcaa cgcttccgct gcttcaggaa cgcgatcgac
     3901 attaatcatt tcaaaatcaa acccccggtt ttccatcgcc cgtttggtgg cgtggcactg
     3961 aacgcaatcg ttacgagtgt aaatagttat gcgcatgatt cgtatttccg tttaaaatga
     4021 agatacggcg cgatgatacg cgtcgggttg tctctctgtt gatacagaga tactagatgt
     4081 agttgaaaaa agattcaact acacaatata tagcaaatag tgatcgaaat taccctggat
     4141 atgagcgtga cgggggaggg ggatttttgt gattcaccag gcaaaaagaa accccgaaga
     4201 caggcttcgg ggtcaaagac gcgtatttat tatcattttt gcactacgat ttgcgcatgc
     4261 ttaacagtgc gccgataaaa atacctaccg cagctgctgt acccacgcta caccagggtc
     4321 tttcacgaac aaaagaatcc gcgcagccaa cggcatcgcg cgcggcttgc tggacgcgag
     4381 tacgaccatg cattcgcgcc ctggtttctt tcagcaacgc ctgagcttta ctgcgtgcgg
     4441 cttcagcttc ccctttggcg tcgctgcccc aggacttcaa tacagattcg aggctgtcag
     4501 ctaattgatt gacatcattc tggatatcct gcacgccatc atcaacgtcg ttgcggttcg
     4561 gtcggttaaa catatgatcc cccattagaa ttcgatgtaa ttttcagtgt agaccataat
     4621 tttcttaact gaggttcatc gcggctttat ggacttttct gaaagcgttg tgaatgcgca
     4681 atcaggtaag gtaggcccg
//
LOCUS       DAZPEB010000233         4673 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZPEB010000233 DAZPEB010000000
VERSION     DAZPEB010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4673
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.238"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            60..1859
                     /gene="cysJ"
                     /locus_tag="ACHURJ_003785"
     CDS             60..1859
                     /gene="cysJ"
                     /locus_tag="ACHURJ_003785"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFR1282550.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKD
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDIEAADEFLSELRVERRYQRDVY"
     gene            1859..3571
                     /gene="cysI"
                     /locus_tag="ACHURJ_003786"
     CDS             1859..3571
                     /gene="cysI"
                     /locus_tag="ACHURJ_003786"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFR1282551.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPHHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            3646..4380
                     /gene="cysH"
                     /locus_tag="ACHURJ_003787"
     CDS             3646..4380
                     /gene="cysH"
                     /locus_tag="ACHURJ_003787"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFR1282552.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
BASE COUNT         1153 a         1217 c         1334 g          969 t
ORIGIN      
        1 tgttttatgg gcgctgacag ggcgcagaaa cagctttgct tactggaaca taacgacgca
       61 tgacgacaca ggtcccacct tccgcgttgc ttccgttgaa cccggagcaa ctggcacgcc
      121 ttcaggcggc cacgaccgat ttaacgccta cccagcttgc ctgggtttct ggctatttct
      181 ggggcgtact caatcagcag cctgctgcgc ttgcagcgac gccagcgcca gccgcagaaa
      241 tgccgggtat aactattatc tccgcctcgc aaaccggcaa tgcgcgccgg gttgctgaag
      301 cattacgcga tgatttatta gcagcaaaac tgaacgttaa gctggtgaac gcgggcgact
      361 ataaattcaa acaaatcgcc agcgaaaaac tgctcatcgt agtgacgtca acgcaagggg
      421 aaggggaacc gccggaagaa gccgtcgcgc tgcataagtt cctgttctcc aaaaaagcgc
      481 caaagctgga aaacaccgcg tttgccgtgt ttagcctcgg cgatagctct tatgaatttt
      541 tctgccagtc cgggaaagat ttcgacagca agctggcgga actgggtggt gaacgcctgc
      601 tcgaccgtgt cgatgccgat gttgaatacc aggctgctgc cagcgagtgg cgcgcccgcg
      661 tggttgatgc gcttaaatcg cgtgcgcctg tcgcggcacc ttcgcaatcc gtcgctactg
      721 gcgcggtaaa tgaaatccac accagcccgt acagcaaaga cgcgccgctg gtggctagcc
      781 tctctgttaa ccagaaaatt accgggcgta actctgaaaa agacgttcgc catatcgaaa
      841 ttgacttagg tgactcgggc ctgcgttacc agccgggtga cgcgctgggc gtctggtatc
      901 agaacgatcc ggcactggtg aaagaacttg tcgaactgct gtggctgaaa ggcgatgaac
      961 ctgtcaccgt cgagggcaaa acgttgcctc tgaacgaagc gctacagtgg cacttcgaac
     1021 tgaccgtcaa caccgccaac attgttgaga attacgccac gcttacccgc agcgaaacac
     1081 tgctgccgct ggtgggcgat aaagcgaagt tacagcatta cgccgcgacg acgccgattg
     1141 tcgacatggt gcgtttctct ccggcgcaac tggatgccga agcgctgatt aatctgctgc
     1201 gcccgctgac cccgcgcctg tattccatcg cctcctcgca ggcggaagtc gagaacgaag
     1261 tacacgtcac cgttggtgtg gtgcgttacg acgtggaagg ccgagcccgt gccggtggtg
     1321 cctccagctt cctcgcggat cgcgtggaag aagagggcga agtccgcgta tttatcgaac
     1381 ataacgataa ctttcgcctg cccgctaacc cggaaacccc ggtgattatg attggcccag
     1441 gcaccggtat tgcgccgttc cgcgccttta tgcagcaacg cgccgccgac gaagcgccag
     1501 gtaaaaactg gctgttcttt ggtaatccgc actttacgga agacttcctg taccaggtgg
     1561 agtggcagcg ctacgtcaaa gatggcgtgc tgacacgtat cgatcttgcc tggtcgcgcg
     1621 accaaaaaga aaaagtttac gtacaagaca aactgcgcga acagggcgcg gagctgtggc
     1681 gctggatcaa tgatggtgcc cacatttatg tctgcggcga cgctaatcgc atggcgaaag
     1741 acgttgagca ggcacttctg gaagtgattg ccgaatttgg tggcatggac atcgaagcgg
     1801 cggatgaatt tttaagtgag ctgcgcgtag agcgccgtta tcagcgagat gtctactaat
     1861 gagcgaaaaa catccagggc ctttagtggt cgaaggaaaa ctgacagacg ccgagcgcat
     1921 gaagcttgaa agcaactacc tgcgcggcac cattgcggaa gatttaaacg acggtctgac
     1981 cggcggcttt aagggcgaca acttcctgct gatccgcttc cacggcatgt atcagcagga
     2041 tgaccgcgac atccgcgccg aacgtgctga acagaagctg gagccgcacc acgcgatgct
     2101 gcttcgctgt cgtctgccgg gtggggtgat taccactaaa cagtggcagg cgatcgacaa
     2161 atttgccggt gaaaacacca tctatggcag cattcgcctg accaaccgcc agacattcca
     2221 gttccacggc attctgaaaa agaacgtcaa accggtgcac cagatgctgc actcggtcgg
     2281 tcttgatgcg ctggcgacag ctaacgacat gaaccgtaac gtactctgca cctcgaaccc
     2341 ttacgagtcg cagctgcacg cggaagcgta cgagtgggcg aagaagattt ctgagcatct
     2401 gttgcctcgt acccgcgcgt atgcggagat ctggctcgac caggaaaaag tcgccactac
     2461 tgatgaagaa ccgatccttg gtcagaccta cctgccgcgt aagttcaaaa ccacggtagt
     2521 gatcccgccg cagaacgata tcgatctgca tgccaacgac atgaacttcg tggcaatcgc
     2581 cgaaaacggc aagctggtag gcttcaacct gctggtgggc ggtgggcttt ctatcgaaca
     2641 cggcaacaag aaaacttacg cccgcacggc gagcgagttt ggctatctgc cgctggagca
     2701 tacgctggcg gtggccgaag ctgtcgtgac aactcagcgt gactggggta accgaaccga
     2761 tcgtaaaaat gccaaaacca aatacacgct ggagcgcgtg ggggttgaga cgtttaaagc
     2821 ggaagtggag cgtcgcgcgg ggatcaaatt tgaaccgatc cgtccatatg agttcaccgg
     2881 acgaggcgat cgtattggct gggttaaggg cattgatgat aactggcacc tgacgctgtt
     2941 tatcgaaaat ggtcgcatcc ttgattatcc ggggcgtccg ctgaaaaccg gcctgctgga
     3001 gatcgcgaag atccacaaag gcgatttccg cattacggcg aaccagaatc tgatcatcgc
     3061 cggtgtaccg gaaagcgaga aagcgaagat cgagaagatc gccaaagaga gcgggttaat
     3121 gaatgccgtc acgccgcagc gtgaaaactc gatggcctgc gtgtcattcc cgacttgccc
     3181 gctggcgatg gcggaagcag agcgtttcct gccgtctttt atcgacaaca tcgataattt
     3241 aatggcgaaa catggtgtca gcgatgagca tatcgtgatg cgtgtaacag gctgcccgaa
     3301 cggttgtggt cgcgcgatgc tggcggaagt gggcctggtg ggtaaagcgc cgggtcgcta
     3361 caacctgcat cttggcggca accgcattgg gacacgtatc ccacggatgt ataaagaaaa
     3421 catcaccgag ccggaaatcc tggcgtcgct tgatgaactg atagggcgct gggcgaaaga
     3481 gcgcgaagcg ggtgaaggct tcggcgactt tacggtgcgt gcgggcatca ttcgcccggt
     3541 gctcgatccg gcgcgcgatt tgtgggatta accatcagcc cggtcttgta ggcctgataa
     3601 gaacgcgtga gcgtcgcatc aggcaaggca aacagtgagg aatctatgtc caaactcgat
     3661 ctaaacgccc tgaacgaact gccgaaggta gatcgcattc tggcgctggc ggaaactaac
     3721 gccgaactgg aaaaactgga cgctgaaggc cgcgtagcct gggcgctgga taatctgccc
     3781 ggtgaatatg tactttcttc cagctttggc attcaggcgg cggtgagcct gcatctggtg
     3841 aatcaaattc gcccggatat tccggtgatc ctcaccgata cgggttactt gttcccggaa
     3901 acctaccgct ttattgacga gttaacggac aaactcaagc tcaacctgaa agtgtaccgt
     3961 gctaccgaaa gcgcagcctg gcaggaagca cgctacggaa aactgtggga gcagggcgtt
     4021 gaaggcattg aaaagtacaa tgacatcaac aaagtcgaac cgatgaaccg ggctctgaaa
     4081 gaacttaacg cgcaaacctg gtttgctggc ctgcgccgtg aacaatccgg cagtcgcgcc
     4141 aatttaccgg tgctggcaat tcagcgtggc gtatttaaag tgctgccgat tatcgactgg
     4201 gataaccgaa ctatttatca gtacctgcaa aaacatggcc tgaaatatca cccattatgg
     4261 gatgaaggat atttatcggt cggtgatacc catacaaccc gtaaatggga acctggcatg
     4321 tcggaagaag aaacacgttt ctttggctta aaaagggaat gtggattgca cgaagggtaa
     4381 atattgccgg ataaattaaa cgcctctgtc agaaatgatg ggggcgtttt tattttgcga
     4441 gcagtattcc tgattttaaa cgtgaacgcc gctggaagaa ttatcaggta tagaatggcg
     4501 tcgggtgctt gaggctgtct ggcgttaagc attcgcgagg ttccagatgg acaaaagccc
     4561 caggcgatat ttctatcaac ctgaggccag cgttcgaacc caaacaattc gaatgttagt
     4621 ctcttctttg cagggaggca agacatgctg acaaaatatg cccttgtggc aat
//
LOCUS       DAZPEB010000234         4662 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZPEB010000234 DAZPEB010000000
VERSION     DAZPEB010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4662
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.239"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..755)
                     /locus_tag="ACHURJ_003788"
     CDS             complement(<1..755)
                     /locus_tag="ACHURJ_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CSS-motif domain-containing protein"
                     /protein_id="HFR1282553.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLQQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLL"
     gene            complement(886..2250)
                     /gene="sdaA"
                     /locus_tag="ACHURJ_003789"
     CDS             complement(886..2250)
                     /gene="sdaA"
                     /locus_tag="ACHURJ_003789"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFR1282554.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(2434..3012)
                     /locus_tag="ACHURJ_003790"
     CDS             complement(2434..3012)
                     /locus_tag="ACHURJ_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFR1282555.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVIGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            complement(3016..4377)
                     /gene="pabB"
                     /locus_tag="ACHURJ_003791"
     CDS             complement(3016..4377)
                     /gene="pabB"
                     /locus_tag="ACHURJ_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFR1282556.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVAEPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVTKILRQ
                     LEK"
     gene            4451..4630
                     /locus_tag="ACHURJ_003792"
     CDS             4451..4630
                     /locus_tag="ACHURJ_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFR1282557.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
BASE COUNT         1170 a         1198 c         1212 g         1082 t
ORIGIN      
        1 cttagcagca ccgccagcgg taattgtgtt ggaagatagt gccatgccag cgccgtagcc
       61 cccggaccat taacgttaat ggtaaaagga aagcgttgcg aagaaacctg atagttttca
      121 ttgtcttcag gttgcggaag ggaatctacc agcccattac catagagcaa atgccgtttg
      181 tccaccgtca ggctggcgct actgatttgc tgcagttgtg gttcaaggag cattgccgtc
      241 agtaagtcga tgttaatcat ctccatgacc ccagcattgc tactgcccgc tgcaggcgtc
      301 cattgaatca aaaccggact gcctttaatc agggcacggt cgatcgtcag gcgtaaaagt
      361 ggttgcggtg caggaagttc agccagaatg tccacgacgg gcacattgcg ataaccaaaa
      421 atgctggagc aatagagtgt gccgtcttgc accaggccaa tggatcgcac agtttggagt
      481 tttgccgcct gtttacgtaa tggcaaatgg gcgacagagc agggcagacc aatcagcgga
      541 agcaagactt cgctacctgc ctgtagggga agcagtactt tatctaattc ctctacagcg
      601 tgattggcga attgtactac ccgttgttga tttaagttac gctgtgaaat aaatcgcacg
      661 ctcagggtcg aagcgagcgt aacgagtgcg caaatcgtac aaacaatcat tcgattacgt
      721 cgataggttt taatgatccg ttgtgctttt tgcatgcggg ccaccctgta agcgtatggc
      781 gacaaacgcc tgacgcaaca gtggatgtgt agtggggaat attacagcag acgagaaagc
      841 gggtataaat tcgcccatcc gttgcagatg ggcgagtaag aagtattagt cacactggac
      901 tttgattgcc agaccaccgc gtgaggtttc gcggtatttg gcgttcatgt ccttaccggt
      961 ttcgtacatc gtttcgatga ccttatccag cgagacgcgc ggtgcactgg tgcggcgcag
     1021 agccatccgt gcggcgttaa tcgccttcac agaggcaatg gcattacgct caatgcacgg
     1081 cacctgaacc tgccctgcaa ccgggtcgca ggttaaacca aggttgtgtt ccatgccaat
     1141 ttccgccgcc acgcaaacct gttccgggct accgcccagc agttctgcaa gacccgcagc
     1201 agccattgaa caggcaacac ccacttcgcc ctggcaacca acttccgcac cggaaataga
     1261 ggcgttcatt ttatacaatg caccaatcgc gcccgctgcc ataaagtaac gggtatagat
     1321 gtccgggctg accgattcaa taaagtggtc atagtaagcc agcactgccg gaacgatacc
     1381 gcaggcaccg ttggttggcg cagttaccac acgaccaccg gcggcgtttt cttcgttaac
     1441 tgccagcgca aacatgttta cccagtcaat gacattcatc ggatcgttag acagtttatc
     1501 gctggaaacc agcatccggc gcagggcaga cgcacgacgt ggcacgcgca gcgggcctgg
     1561 cagtacacct tcggtgttca tcccgcgatc gatacatgcc tgcatggttt gccagacatg
     1621 cgcgaaatac tcgtcgatct ctttcttgct gtgcagcgcc agttcgttct gcatagcgag
     1681 accagacagc gaatagccgg tttcattaca gtacgcgagc agttcggtgg cagatttgaa
     1741 cggatacggc acgctcactt cgttggcagc atcctgacca aagtgttctt catcgacgat
     1801 aaaaccgccg ccgatggaat aataagtttt gctgtagacg acttcatcgc cgttataggc
     1861 gtggatttgc ataccgtttt catgcagcgg caggttgccg ttatgaaaac gcatcccgtt
     1921 gtcgcgcggg aaatccactt catgccgtcc ctgtgccagc agcagacgtt cgcgctcttc
     1981 tacgtcgcga ataaaaccgg gaatactgtc gatatccacg gtggcaggtt cgttacctgc
     2041 aagacccata ataatggcga tatcggtgtg gtggccttta cccgtcagcg acagtgaacc
     2101 ataaacgtcc acggcaacgc gagtaacgct atccagtaag cctttttcga ccagatcatc
     2161 gacgaactgt ttacccgcct tcataggccc tacggtatgg gaagatgagg gaccaatccc
     2221 caccttaaac atgtcgaata gactaatcac gataatactc ctgacaaggt gactggactt
     2281 ccagtaacga actaataaca gcgcatagtg taagagggaa cgcggcgact ggcttaacta
     2341 ttcacatgaa ttaaactaat caaaaaccgc gatttaacta atattgcgat gattgtctca
     2401 agtagtgttt tgtagatgta aagataagta tagtcagggt ttcacaccaa tttgcagcgc
     2461 cagctcacga attatgcctg cggtcattcc ccatacaaaa tactgttcgt accaggacag
     2521 ccatacccga tgtgaatcgc cacggcggta gatatctaaa gggtgataac gacccagatg
     2581 taatgcctgg gcgagcggca tttcaaacac cgccgagact tcatcttcac tggcgcgata
     2641 cggcagatcg ggcgggataa tgccgatcac tggggttacc tgatagccag ttacgctatc
     2701 gacgggcggc agcacgccga taacttcaac ggcggaaggc ggtatagcga cctcttcttc
     2761 agcttcgcgc agcgcggcgg cgataactga tgcgtccgtg tcatcgactg cacctccagg
     2821 gaatgccact tgtccagcgt gtttacgcag atgaattgaa cgctgagtca gcaacaaccc
     2881 cggttgcggt cgacggacga tggggattaa cacggcagcc tgacgatgat ttagggtttc
     2941 ccggttaatt tgcgggcgca aaagttgaaa gcgagataaa aaatcatcaa gcgtcaggct
     3001 acggtattcc acgtcttact tctccagttg gcgtaatatc ttagtaactt tatcaaaagt
     3061 ttcctgatat tccgcttctt cctggctatc ggcgacaatt ccaccgcccg cagagcagta
     3121 tatttgtccg ttaatggcag tcagcgtgcg gatagtaatg ctggtatcca tgttgccgca
     3181 aaagctcaaa tagccaatgc tgccgcacca ggcattacgt cgctgcggtt ccagttcgtc
     3241 gataatttcc atagcccgta ctttcggagc cccggttatt gagccaccag gaaaagctgc
     3301 gcgcagcaga tcgctggcgt gtaactgttc tggtagtcgc gccgttatag tgctgaccag
     3361 atgatgcacg gcagggaagg gttccaccac gaagagctct ggtactttta ccgaaccggc
     3421 tacggcaaca cgaccgatat cattacgcat taaatcgaca atcatcagat tttcggcacg
     3481 atctttcgct gagttcgcca gtttttctgc ttgtttgcta tcttcctgag gatcgggcag
     3541 gcgtggtagc gtgcctttaa tcgggcgggt ctggatttca ctattatcac aaagaataaa
     3601 ccgctctggc gaaaggctta aaattgcacc ctgttcaaga cgtaaaaaag cgctaaatgg
     3661 cgcgcggttg gcctgattaa gctgaaggaa tgcctgccat tcatcgccag aataggtcgc
     3721 atgaaaacgc tgggcgagat tcacctgata gcaatcaccg ctgtgcagat attcctgtac
     3781 ctggcgaaat ttttcgccgt actgctcgcg ggtcatattg gattgccagt cggaagtgag
     3841 cgtgaaatct tcctgcggcg agaattgctg gctttccagc caggcccgac gggcattgac
     3901 atcattatga ctcagcaaag aaactgtatg acgctggtgg tcgacaatga gcgcccaatc
     3961 gtagataccc actgccatat ccggcagaac gatatcttgt tccgcaattt ctggcagtga
     4021 ctcaaaacgg cggcccagat cgtagccaaa caaccccagt gcgccgccct gaaatggcaa
     4081 atcttcgtta tgcgttgggc gaatgtctgc gcgatccagc acctgctgga gcacctgtag
     4141 cgggtcatca gtggtcgttg tgcgtttttc gctttcacta acaacggttt ctttaccgaa
     4201 agtggttaaa gtgcaaatcg gctcggcgac cacaatatca aagcggctat acggatgatc
     4261 ggcatagccg gagtgtaaaa gcatcgccca cggcaggtgg cttaagcggg agaaataaaa
     4321 ttcagcggcg tcctgacgcc agggtaaagt aatcacagcg ggagataacg tcttcatcag
     4381 tcctgactct actggctatg tgccgggtag catgaaataa ttagcgccaa caatttagca
     4441 ggagttaaca atgtttgcag gtttaccttc actcacccat gaacagcagc aaaaagctgt
     4501 cgagcggatc caggaactga tggcacaagg gatgagcagc ggccaggcaa tcgcgctggt
     4561 ggcagaagag ctgcgtgcca accactccgg cgagcgaatc gtggcgcgtt ttgaggatga
     4621 agacgagtaa cacactaatc cgtatcgatt tcgttataga ag
//
LOCUS       DAZPEB010000235         4588 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZPEB010000235 DAZPEB010000000
VERSION     DAZPEB010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4588
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.240"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            256..618
                     /gene="queD"
                     /locus_tag="ACHURJ_003793"
     CDS             256..618
                     /gene="queD"
                     /locus_tag="ACHURJ_003793"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFR1282558.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            696..1964
                     /locus_tag="ACHURJ_003794"
     CDS             696..1964
                     /locus_tag="ACHURJ_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR1282559.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGVTTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWQLVGDALRSCVNTGISVRGMGMALTGAQAAQTLISACQHREP
                     QNLFALYHHNVERSLLWDVLQRYQHVPALLQRPSWYRAWPALMQDISRDLWDQGDKPV
                     PPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            1955..2215
                     /locus_tag="ACHURJ_003795"
     CDS             1955..2215
                     /locus_tag="ACHURJ_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFR1282560.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQWLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            2232..2807
                     /locus_tag="ACHURJ_003796"
     CDS             2232..2807
                     /locus_tag="ACHURJ_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFR1282561.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDACNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(2955..3815)
                     /locus_tag="ACHURJ_003797"
     CDS             complement(2955..3815)
                     /locus_tag="ACHURJ_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFR1282562.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGIQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(3812..>4588)
                     /locus_tag="ACHURJ_003798"
     CDS             complement(3812..>4588)
                     /locus_tag="ACHURJ_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFR1282563.1"
                     /translation="NILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADEQ
                     AAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPEF
                     VARHIAEWQHQNPLDLIITGSQSSEGQNGQTPFLLAEMLGWTCFTQVERFTLDALFIT
                     LEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPAM
                     QCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
BASE COUNT         1056 a         1234 c         1166 g         1132 t
ORIGIN      
        1 agaagataag gttaacgggg tgaacggtgt ggattaaaga cgggatagcg ataactaata
       61 accaaatcga ctaacctgtt ttagcaatag tctttaacaa caaaatagat taaccaaccc
      121 aatgaaaaac aaatgaattt agccaatcat taagataaat cagcgatttt gcgcaacaaa
      181 gtcgttttag ataatgcgaa aaaacagcct ttccggtact ctacggcggt tttatcgtcc
      241 tgtagagaaa ttatgatgtc caccacgtta tttaaagatt tcaccttcga agccgctcac
      301 cgcttaccac acgtcccgga agggcataaa tgtggtcgcc tgcacgggca ttcctttatg
      361 gtgcgactgg aaattaccgg ggaagtcgat ccgcatacgg gctggattat cgatttcgct
      421 gaactaaaag cggcgtttaa gccaacttat gagcgcctcg atcaccatta tctcaatgat
      481 attccaggtc tggaaaaccc aactagcgaa gttttagcaa aatggatttg ggatcaggtt
      541 aaacccgttg tgccgctgtt aagtgcggtg atggtaaaag aaacctgcac cgctggttgt
      601 atctatcgcg gcgaatgata agagtgtgtc ggcggtcaat ttcccttaag taacgctatg
      661 ttagggtatt gtgttcagga tatctggggc atgacatgga agacgactgc gacattatta
      721 ttattggtgc cggtattgca ggcaccgctt gcgcgttacg ctgcgcgcga gcgggtttat
      781 ccgttttgtt actggaacgc gctgaaatcc ccggcagcaa aaatctttcc ggcggacggt
      841 tatataccca tgcactcgcg gaactcctcc ctcagtttca tctgaccgcg cctcttgaac
      901 gacgcatcac tcacgaaagc ctttccctgt taacgcccga tggcgtaacg acgttttcca
      961 gcttacagcc cggcggtgaa tcctggagtg tattacgtgc acggttcgat ccgtggctgg
     1021 ttgccgaagc cgaaaaagaa ggtgtcgaat gtatccccgg agcgacggtg gatgcactgt
     1081 atgaagaaaa cggcagagtc tgtggcgtta tttgtggtga cgatattctc cgcgcccgtt
     1141 atgtggtgct ggcagaaggt gccaacagcg tcctggctga acgtcacggg ttagtgactc
     1201 gtcctgctgg cgaagcgatg gcgttgggga tcaaagaagt gctgtcgctg gaaacatccg
     1261 ctattgaaga acgttttcat ctggagaata acgaaggcgc agcgttgctg ttcagcggcg
     1321 gaatctgtga tgacttaccc ggcggcgcat ttctttatac taatcaacaa acgctctcgt
     1381 tagggattgt ttgcccgctc tcttccctta cgcaaagtcg tgttccggca agcgagctgc
     1441 tgactcgctt taaagcgcat ccggcagtgc gcccgcttat caaaaacacg gaatcactgg
     1501 agtatggtgc gcatctggtg ccagaaggtg gcttgcacag tatgccggtg caatacgccg
     1561 gtaacggctg gcagctggtg ggcgatgcgt tgcgcagttg cgtcaatacc ggaatttccg
     1621 tgcgcggcat gggtatggcg ctgactggcg cgcaggcggc acaaacgctg ataagcgcct
     1681 gccagcaccg cgagccgcaa aatctgtttg cgctttatca tcacaacgta gagcgcagcc
     1741 tgctatggga tgttctacaa cgttatcagc atgttccggc gcttttgcaa cgccctagct
     1801 ggtatcgggc gtggcctgcg ttaatgcagg atatttcccg cgatttatgg gatcagggtg
     1861 ataaacctgt tccaccgctg cgccagttat tctggcgtca tttacgtcgt catggcctgt
     1921 ggcatctggc gggcgatgtt atcaggagtc tgcgatgtct gtagcccata atctctggcg
     1981 cgttgctgat gcgccgcaca ttgttccggc tgactccgtt gagcgtcaga tggtacaatg
     2041 gttgattaac gcctgtccgg caggtctttt ttcgctcaca ccggaaggtg acttacgcgt
     2101 tgattatcac ggctgcctgg agtgtggcac ctgccgtttg ctgtgcgacg aatccacact
     2161 acaacagtgg cgctatccgc cttccggatt cggcatcacc taccgctttg gataaataat
     2221 aaggataatt tatgcccctc ttacacctgc tccgccagaa tccggtgatt gctgccgtta
     2281 aagataatgc cagcctgcaa ctggcaatcg actctgaatg ccagtttatt tccgtgttgt
     2341 acggcaatat ctgcaccatc agtaatatcg ttaaaaagat taagaacgcc gggaaatatg
     2401 cttttattca tgttgatttg ctggaaggcg cgtcaaataa agaagtcgtg attcagtttt
     2461 tgaaactggt gaccgaggcg gacggcatta tcagtaccaa agcctcaatg ttgaaagcgg
     2521 caagagcgga aggttttttc tgtattcatc gcctgtttat tgttgattca atttcgtttc
     2581 acaacattga taagcaagtt gcgcaatcga atccggattg tattgagatc ctgccaggct
     2641 gtatgcccaa agtgctgggc tgggtgacag agaaaatccg ccaaccgctg attgccggtg
     2701 ggctggtgtg cgatgaagaa gatgcgtgta atgcgattaa cgcgggtgtc gtggcgcttt
     2761 ccaccacgaa taccggggtc tggacgttag cgaaaaaatt actttgacgg gataatcgta
     2821 accaattgaa tttggtttga tttgtaggcc gcacgccaca tccgacattc agcgcctgat
     2881 gcgacgcttg acgcgtctta tcaggcctac aagtccccag gccagaaccg taggcaggat
     2941 catccggcaa cacattattg gcaatctgcg tgaatgtttg tcaccagtgc ctccagcacc
     3001 actttccagt catcaaccac gccaacatct gcctgtgaaa ataccgcagc gctggcgtca
     3061 tgattaatcg ccaccacaaa cttgctattg cgtacgcccg ccattaacgc tgctgcgccg
     3121 gaagcgccaa caacaataca cacctcaggt gccagcaaat gtccggaaat accaatcact
     3181 ttttcagcat caacgccacc gttcatcact cgcgcccgac tgtagcccac ttcggctccc
     3241 agcttttctg ccagcatagc gatttcctgg ttatctgcct ctccgccctg ccccaccacc
     3301 agcacccgtc tggcctcagc taatgggtcg cgagtaacat ttttcagatc ctctgtgcta
     3361 accagccagt cgggaagcgc acccgggaca atgttcagtt gttgtattcc agagggcaac
     3421 gtggcgtttt tcgctgctcc cgcctgacgc gccagagaaa ggcatagcgg gcgtttttct
     3481 gtttgcagcg tcgccgttag cgcatttccc cagtgggatt tgcgcacgct tacggttgga
     3541 atatctaacg aagtcacctg gcagatgctt gcaccatgta aacgccaggc cagccgggtc
     3601 gacagttcat cgccaaacgt tcccggcgga aagagaacaa cgtccgcagg cgttcgctgc
     3661 cactgttcca ccaacgcatc cagcacctgt tccgccacta caggctgagg ttcaatctgc
     3721 cagtgcgcca gggtacaacc actgaaatcc tgcgccgcca gccagcttgc tattgcggca
     3781 ttctcctgat ttattgtcac aattgcgata ttcatggctg catcctttgg cgtaaatagt
     3841 cctgccatag cttttgagct ttttctgcta ccgtttgccc atcaatcagc gtcgctccgc
     3901 gtcgttgctc tgctcttgcc agttgcagac actgcatcgc tggcatttcg gcagcaacag
     3961 ttttacggat aatttctgct ttccccgcag ccattcgctg gcgcattccc ggtactggca
     4021 aggccacttc accgcactga cgcacggcaa tcaccgcagg caagcgaacc cggcaacaac
     4081 gcaacccatg ttcagtacgc tgttcgaggg tgataaacag cgcgtcgaga gtgaaacgct
     4141 ccacctgggt gaagcaggtc cagcccagca tttccgccag caaaaatggc gtttgcccat
     4201 tctgcccttc gctgctttgg ctgccggtaa ttatcagatc cagcggattc tgatgctgcc
     4261 attcggcaat atggcgagcg acaaattccg gcgcaaagcg cagatctgcc gccgtctcca
     4321 gcaatacagc ttcctcaaac ccaagagcca tgagatagcg cagccagtgc aatgcccgtt
     4381 catcccccat gctcaacgcg gttaaagaca tcggcgtacc gttttttctc tgcgccaaca
     4441 gcagcgcggc agcggcctgt tcatcagcac cgagtaaact tcgcagtagc gaaatatccg
     4501 gtccgctttt accctgagcc gccgcctgcc actctttttc cgccagcatt ccggcatccg
     4561 gttcggcttt aaacgctaac agaatgtt
//
LOCUS       DAZPEB010000236         4570 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZPEB010000236 DAZPEB010000000
VERSION     DAZPEB010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4570
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.241"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            157..873
                     /gene="nanC"
                     /locus_tag="ACHURJ_003799"
     CDS             157..873
                     /gene="nanC"
                     /locus_tag="ACHURJ_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFR1282564.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSSDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            893..1999
                     /gene="nanM"
                     /locus_tag="ACHURJ_003800"
     CDS             893..1999
                     /gene="nanM"
                     /locus_tag="ACHURJ_003800"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFR1282565.1"
                     /translation="MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNREGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAVDKINAYYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQHGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            2064..3044
                     /locus_tag="ACHURJ_003801"
     CDS             2064..3044
                     /locus_tag="ACHURJ_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFR1282566.1"
                     /translation="MDAIISPDYYYVLTIAGQSNAMAYGEGLPLPAREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLSFIPDNAGVLIVPCCRGGSAFTEGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLIMGYYGSAYRSPENWTTALRSSHFSAAARRGIISDRFVEAILQFWRGK"
     gene            complement(3052..3156)
                     /locus_tag="ACHURJ_003802"
                     /pseudo
     CDS             complement(3052..3156)
                     /locus_tag="ACHURJ_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            3256..4128
                     /locus_tag="ACHURJ_003803"
     CDS             3256..4128
                     /locus_tag="ACHURJ_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorothioated DNA-binding restriction
                     endonuclease"
                     /protein_id="HFR1282567.1"
                     /translation="MASSKSLQQAIANIKIWHKGEQRAPHKPLLLLYVLAGYLNGHPR
                     LFDYGSEIYEPLHSLLERFGPQRSQYRPDMPFWRLQGDGFWQLHNAELCSTAGSSRQP
                     PVKELNEYHVAGGFDEQHYALVTGNKKLINTLAQQILEAHFTESIQEEIADELGFDLQ
                     QIRKQRDPLFRKNVLRAYNYQCAICGFNMRHDDTTVALEAAHIKWKQHGGPCEIPNGL
                     ALCAIHHKAFDKGSIGLDENMRVLVSDAVNGGGSVERLFWDFDGKIIALPQVRKNYPY
                     EGFVEWHRNEVFRG"
     gene            4173..4352
                     /locus_tag="ACHURJ_003804"
                     /pseudo
     CDS             4173..4352
                     /locus_tag="ACHURJ_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
BASE COUNT         1340 a          975 c         1066 g         1189 t
ORIGIN      
        1 gatttttcac aggagacctt atacaaaaaa atataaaata cagctaccgg ttgccaaaga
       61 cactataagc ctggcaaaaa atattacaca acataaatgc taattgttta tgcgggcttt
      121 gtattgcttt ctgtatccta caaatgagtg aaatttatga aaaaggctaa aatactttct
      181 ggcgtattat tactgtgctt ttcgtcccca ttaatttctc aggctgcgac actggacgta
      241 cgtggtggat atcgtagtgg aagccacgcc tatgagactc gactcaaagt cagtgaggga
      301 tggcaaaatg gatggtgggc aagcatggaa agtaatacct ggaataccat tcatgataat
      361 aaaaaggaaa atgccgcact caatgatgtt caggttgaag ttaattacgc gattaaactt
      421 gatgatcaat ggacggtgcg cccgggaatg ttaacgcatt ttagcagcaa cggcacacgc
      481 tacggaccct acgtaaaact gtcctgggac gcgacaaaag atcttaattt tggcattcgc
      541 tatcgttacg actggaaagc ttaccgacaa caagacttat ccagtgatat gtctcgtgat
      601 aacgttcatc gttgggatgg atatgtcact taccatatta atagtgattt caccttcgca
      661 tggcaaacga cgctatacag caaacagaac gattatcgct atgcaaacca taagaaatgg
      721 gcgacggaaa atgcatttgt tctacaatac catatgacgc ccgatattac gccatacata
      781 gaatatgact accttgaccg tcagggtgtt tacaacggca gagataattt atcggaaaac
      841 agttatcgca ttggtgtgtc atttaaactg tagtagacag gagacagtca caatgaataa
      901 aacaataacg gcgcttgcta tcatgatggc ttcatttgcc gcaaacgcgt ctgtattacc
      961 ggaaactcct gtgccattta aaagtggtac cggagcaatt gataacgaca ctgtctacat
     1021 tggtttaggt agcgcaggta cggcatggta caagctggat acacaggcca aagataaaaa
     1081 atggacagcg ttagctgcat tccctggtgg accaagagat caagcaacct ctgcatttat
     1141 tgatggcaat ctgtatgtgt ttggcggcat tggcaaaaac agagagggct tgactcaggt
     1201 atttaatgac gtacacaaat acaaccccaa aaccaatagc tgggttaaat tgatgtcgca
     1261 cgcaccgatg ggcatggcgg gtcatgtgac ttttgtacac aacggcaagg cttatgttac
     1321 tggcggtgtt aaccagaata tcttcaatgg ctattttgaa gatctcaacg aggctggaaa
     1381 agattcaacc gctgtagata aaatcaatgc ctactatttt gacaaaaaag cagaagatta
     1441 tttcttcaat aagtttctgt tgtcttttga tccctcaaca cagcaatgga gttacgctgg
     1501 cgaatcgccc tggtacggaa cggctggtgc ggcggttgtg aataaaggtg ataaaacctg
     1561 gcttattaat ggcgaagcca aacccggatt gcgaacggat gccgtatttg aacttgattt
     1621 caccggtaat aatttaaaat ggaataagct tgctcccgtc tcatcaccag atggcgtcgc
     1681 tggcggtttt gcggggataa gcaatgattc tcttatattt gccggagggg ccggattcaa
     1741 aggttcacga gaaaattacc agcacggaaa gaactatgcg catgaaggcc tgaaaaaatc
     1801 atatagcact gatattcatc tttggcataa cgggaaatgg gataaatcgg gtgaattatc
     1861 gcaaggtcgg gcctacggag tatcattgcc ctggaataat agtctattga ttattggcgg
     1921 tgaaactgca ggcggcaaag cggtgacgga ttcagttttg atctctgtga aggataataa
     1981 agtcacagta caaaactaac gcttcagggc cccagtaaag gggccctgct atcaatttat
     2041 catttcaatt acgcgaaaat tatatggacg caataatatc gcccgattat tactatgttc
     2101 ttaccattgc tggtcagtct aatgctatgg cgtatggcga aggactgcca ttaccggcca
     2161 gggaagatgc gcctcatccc agaattaaac aattagcgag atttgcgcat acgcatcccg
     2221 gaggcccgtc atgtcacttt aacgacatta ttccactaac tcactgccca cacgatgttc
     2281 aggatatgca gggttatcac catcctctgg caacgaatca tcaaacacag tacggcaccg
     2341 ttggccaggc actgcatatt gcacggaaat tactgtcctt tattcctgat aacgcagggg
     2401 ttctcatcgt tccgtgttgc cgtggcggat cggcttttac cgagggcagc gaagggacat
     2461 attcagaacg gcacggagcc agccatgatg cttgtcgttg gggaacggat actccgctat
     2521 accaggattt agtcagcaga acgcgagccg cactggcaaa aaatccgcag aacaaattcc
     2581 tcggcgtatg ctggatgcaa ggcgaatttg acttaatgac cagtgactac gcgtcacacc
     2641 ctcaacactt taatcatatg gttgaagcct ttcgtaggga tctaaaacaa taccattctc
     2701 agcttaataa tattactgac gcgccgtggt tttgcggcga taccacctgg tactggaaag
     2761 aaaatttccc tcatgcgtat gaagctattt atggcaatta tcaaaataat gttttagcta
     2821 atattatttt cgtcgacttc cagcaacaag gtgaaagagg actgacgaac gcgcctgatg
     2881 aagacccgga cgatttaatc atgggatatt acggttcagc gtaccgctca ccggagaact
     2941 ggacgacggc actgcgaagc agtcatttca gcgcggcagc ccgtcggggg attatttctg
     3001 acaggtttgt agaagcaatt ttgcagtttt ggcgcggaaa gtgagcgttc attaatttga
     3061 tttattcgca tccatcaacg cttttaactc ctccggcgta aacggtggtt tgcgtcggtg
     3121 caacatcgca tggcaattag ggcacacaag cagcaaaaaa acaaatgaga tcaaggtaat
     3181 atatcttcac ttgcaggctt gaacagcaga aaatttgcga gcatgatcgc aattatttca
     3241 ctgctaggga tatcaatggc ttccagtaaa tcgctacagc aggcaattgc caatataaaa
     3301 atctggcata agggtgaaca gcgcgcaccg cataaaccat tgttattgct atacgtatta
     3361 gcgggatacc tgaatggaca tccacgcctt ttcgattatg gatcggaaat ctacgaacct
     3421 ctgcacagtt tactggaacg ttttgggcca cagcgttcac agtaccgacc tgatatgccg
     3481 ttctggcgat tacaaggcga cggattctgg caattacaca atgccgagct ttgctcaaca
     3541 gcagggagca gccgacaacc gccagtgaag gaactgaacg aataccacgt tgcaggcggt
     3601 ttcgatgaac agcactacgc cctggtaacc ggcaataaaa aactcatcaa tactctagcg
     3661 caacagatac tcgaagcgca cttcaccgag agtattcagg aagaaattgc tgacgagctg
     3721 ggttttgatc tccagcaaat ccgtaaacag cgcgacccgt tatttcgcaa aaatgtatta
     3781 cgcgcttata actaccagtg cgcgatatgt ggctttaata tgcgccacga cgacaccacc
     3841 gtcgcgctgg aagcggcgca tataaaatgg aaacagcacg gcggtccttg tgaaattcct
     3901 aatggcctgg cgctttgcgc aattcaccat aaagcctttg ataaaggatc gattggcctg
     3961 gacgaaaata tgcgggtact ggtatcagat gcagtaaacg gcggagggag cgttgagaga
     4021 ttattctggg attttgacgg gaaaattatt gcgttgccgc aggtgcgtaa aaattaccct
     4081 tatgaggggt ttgtggagtg gcatcggaat gaggttttta gggggtgagg ttgataacta
     4141 tcgcagtatg ttcaatattg ctgacataat cagtgcagga tcttgccgct agtttacaac
     4201 tcgccttcga tagcggcgca aaacgagttc tgttgccgat atcctgggct atggatattc
     4261 caacagttcc ggcagagttg tttaccaagt ttcaggtaag tttttactca gaaccggttg
     4321 atgcggttta taaggcgctg gatgtgaatt aacacattcc tcagcttatt tacaaaagga
     4381 ttattcccat ttgatataat atcagtgcca atttactttt ttcaatggaa tatatctcgg
     4441 ctaaataaca ataaaactta tcgctaatgg attaaataaa caattttcta attcatttca
     4501 cggtaatgac tccaacttac tgatagtgtt ttatgttcag ataatgcccg atgaccttgt
     4561 catgcagctc
//
LOCUS       DAZPEB010000237         4477 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZPEB010000237 DAZPEB010000000
VERSION     DAZPEB010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4477
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.242"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            127..425
                     /locus_tag="ACHURJ_003805"
                     /pseudo
     CDS             127..425
                     /locus_tag="ACHURJ_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            507..644
                     /locus_tag="ACHURJ_003806"
                     /pseudo
     CDS             507..644
                     /locus_tag="ACHURJ_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            1014..1691
                     /gene="ospD1"
                     /locus_tag="ACHURJ_003807"
     CDS             1014..1691
                     /gene="ospD1"
                     /locus_tag="ACHURJ_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HFR1282568.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKRNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQSISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            2015..2125
                     /locus_tag="ACHURJ_003808"
                     /pseudo
     CDS             2015..2125
                     /locus_tag="ACHURJ_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2203..2517
                     /locus_tag="ACHURJ_003809"
     CDS             2203..2517
                     /locus_tag="ACHURJ_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282569.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSAQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            2799..3365
                     /gene="ipgB2"
                     /locus_tag="ACHURJ_003810"
     CDS             2799..3365
                     /gene="ipgB2"
                     /locus_tag="ACHURJ_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HFR1282570.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYHISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(3627..3749)
                     /locus_tag="ACHURJ_003811"
                     /pseudo
     CDS             complement(3627..3749)
                     /locus_tag="ACHURJ_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            3892..4446
                     /locus_tag="ACHURJ_003812"
                     /pseudo
     CDS             3892..4446
                     /locus_tag="ACHURJ_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
BASE COUNT         1410 a          880 c          985 g         1202 t
ORIGIN      
        1 atttcaacgt gaaaacttat tttcactata atcttcagga ttcgaagatc ataggagcct
       61 gtacataagt ttgtataatt ctctgatgtt gaactattta atttgatatc aaatgaaggt
      121 gaatttatga atgaaaaaca gttgacatct aataagttgc gggctttagc taatgaacta
      181 gccaaaagtc tcaaaaatcc tgaagatctc agtcagtttg attggatgct gaaaatgaaa
      241 ccgtactcaa tgctaatatg actcatcatc ttgggtgcga gaaaaatcag cttagatctg
      301 gcagcaattc ccgcaatggt tgcttaacga agattatcac tactggagat gaacctctgg
      361 aaatccgtac tctgcgtgat cgtaatggca ctttcgaacc gcaacagctg aagaaaaacc
      421 agccctagat tactaaatct gtcgggcgat ggattaactg gttcaggctg tctggtgctg
      481 aaggcctgaa gtcactgctg tcagtgatgt ggccggctct tcatgagaca ataacccgta
      541 atcatcagtg ccgttcaata tggccactgt tgaaaaaagt tcgtcacttt atggagacgg
      601 ttagcccact tcccggagaa aaacatagtc tggccaaagt gtagcggtat taggctcaca
      661 tatttagatg tagataagcc ggagcaggca gttaaatcta ggcagcatca cagaaatgga
      721 caacgcccct taaggactgg cgtatggcaa tgagctgctt tattatcaag tttggtgatc
      781 gccttgacgg tcacttctga gtgaaggcgc ttgcacagaa tggtaaacgg actcgattat
      841 tgaatcttag ggataagata tatctacgat atgtcaatat acgaaatcga aagaatttat
      901 tggtgaatat agccattcaa tgtggcaatg gtttttttag attagcaata gactaatatc
      961 tattacatta caaaaatagg ttattgtttt tttagcaaaa aggatgaaca aaaatgtcaa
     1021 taaataacta tggattacat ccagcaaaca acaaaaatat gcacctaata ataggcagca
     1081 atactgctaa tgaaaataaa ggaatgaaac gtaatatcat taacgtgaca aataccgcta
     1141 tatcccacgc catcaatgaa gaaaaatcag gggggggata tagtggtgtt tctttcagaa
     1201 aattggccaa aatacagagc atatccattc cgacaaagaa taataaggag tataaccgcc
     1261 ataatttgtt ttcattgatt tggcatggaa atgctgatgc agcgcgtaaa tacggtgaat
     1321 cgctgttggc agccgaaata cccaaagagg aaaaactaga agttcttgca gcacgaaata
     1381 atgctgggga atctgctttg ttcatagctc ttcaagaagg tcattccgct gcgattcaag
     1441 cttatggaga ttttattaaa acttttgatt tatcaccaaa agaaacgatt aaactattgg
     1501 atgtaagaga taatgagggg ttaccaggat tatttctggc cgcagggaaa gggaatatcg
     1561 aggctatgat ggcatatata aatatatgcc atcatagtgg gataaaactt acagaaatag
     1621 cagacagact taacaataat gaacaagaca tgtttaatat tatttctgac aaaatacaag
     1681 agttgtttta agtgtgctaa atagctgcaa agaattgcac ttagaaggag ctctccccga
     1741 attgtgtgat ctgcttaatt tctaaaaaga aacaaacagt taactagact gagcgatact
     1801 acccatagaa ccagtttccc atgacgaacg acactggacg caaaaacaat cataaaaaac
     1861 acaataaatg taatgaacac atcccaccct attaagtaaa gtgcactgtg atagaggaag
     1921 ggaaatacag gaaggtgtgg agccttgcag atttctggca tcatagtgcc caatacggaa
     1981 tgcataaagc cccctcagac tggagactcc acaaatgaaa tatacagtgt ttaattatat
     2041 caagtgcgat tacaatcgct gacgacggca cagttggtgt ggaggtctca gtcctgaaca
     2101 atttgaaaac cagaacctca cttaagcctg tgttcatatt acgtgggtag gatcatactt
     2161 ttatagaagc tgtcagtggc tacgtcccag ctaaatcact tcatgctcca gcgccagaga
     2221 ggcaaggttg ggtttgctca gctgccggtt gattttgttg cgattgaacc ggacagcgcc
     2281 cagggtgtcg gcaaaagagc gaatctcacc aatcgttgtt tcattatccg cattaacgat
     2341 agcttcaaga aacgccaagg ttttatcgaa ttcatcagca acagtggctc cgggcatacc
     2401 gtgaccgttt atacaaaaag gcgttttcag cgtggagttt tcatgaatag cctcaacacg
     2461 aatgtcgtga agccagtgat gtatcggcta cgatcctcag ctgatgccag accataggac
     2521 tgacactacg aacagcaatt ttaatgacgt aaatcttcat aactacaact aacagggcat
     2581 cattatgaat gatgccggtt attttaccgc tgttggtccc ttcatttagg gactgcccca
     2641 agttttttca tactctcttt ctcagagagt ttagtctctg ggattccaga cggtccatca
     2701 ttgagccaag ggagtaaaga actcaaagca tttacagaat tgtttgaatc agctaaaata
     2761 atgacaatgt tactatttta catgacaaag tgattataat gcttggaaca tcttttaata
     2821 attttggaat cagtttatct cacaaaagat atttttcagg aaaggttgat gagattatcc
     2881 gctgtacaat ggggaaaagg atagtaaaaa tatcatcaac caaaataaat actagtattt
     2941 tgagctctgt ttcagaacaa atcggtgaaa atattacaga ttggaaaaat gatgaaaaaa
     3001 aagtctacgt atcccgggtg gtaaaccaat gtattgataa gttttgtgca gaacacagcc
     3061 gaaaaatcgg cgataatctc cgtaaacaga tatttaaaca ggttgagaaa gattatcaca
     3121 tttctctaga catcaatgct gcacaaagtt ccataaatca tcttgtatca ggaagcagtt
     3181 atttcaaaaa gaaaatggac gaactttgtg aagggatgaa tagaagcgtt aaaaatgata
     3241 caacaagcaa tgttgcaaat cttatttccg atcagttttt tgagaaaaat gtgcaatata
     3301 ttgatctaaa gaaattacgt gggaatatga gtgattatat tacaaattta gaatcgcctt
     3361 tctgatgaca gaacatcccg agtaaagaaa ctaagaaaag taaataccca ggaattgcct
     3421 gagcggtgct atatttctcc tcgcaagtaa gtcctaatat attgaattgt tgtatagcca
     3481 gtttttgtat tttcctagct catggatgtg ggatccctct cccctttctc tgctcaaagg
     3541 taagcggctc gtcagaatca tattgatatt tactgagaag tcagatcaac tttccaaggc
     3601 aacagatcgc gtacccggtt tgccggtcag tcctggatat gttcaataac gtaacgcagc
     3661 catttttctg gctccacatt gttcagacgg catgtgccga tcagcgagaa caacaccgct
     3721 gcatgttcgt caccgctgtc ggaacctgcg agtcgagtca gccggagaga gtttcactct
     3781 ccggctgctc acggaaccgt acgtgacact ctcgcgtcat acggctcttg atcactcatt
     3841 cgatgcacgt agtaaatacc agtgagcaaa ttgatcttac ccagcaatac ggtcagttac
     3901 cgcgcacatg gcctgcctgt gtcagaaaat ctgctggagc aggattttta cgccagtggt
     3961 ccgaaccaga agtgggcggg agacatcacg tacttacgta cagatgaagg ctggctgtat
     4021 ctggctgtgg tcattgacct gtggtcacgt gccgttattg gctggtccat gtcaccacgc
     4081 ctgacggcgc aactggcctg cgatgccctg cagatggcgc tgtggcggcg taagagaccc
     4141 cggaacgtta tcgttcactc ggaccgagga agtcagtact gttcagcaga ttatcaggcg
     4201 ctgctgaagt ggcataatct gcgtggcagt atgagcgcaa agggctgctg ctacgataat
     4261 gcctgcgtgg aaagcttctt tcattcgctg aaagtggaat gtctccacgg agaacacttt
     4321 atcagccggg aaataatgcg ggcaacagtg tttaattata tcgaatgtga ttacaatcgc
     4381 tggcgacggc acagttggtg tggcggcctc agtccggaac aatttgaaaa ccagaacctc
     4441 gcttaggcct gtgtccatat tacgtgggta ggatcac
//
LOCUS       DAZPEB010000238         4436 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZPEB010000238 DAZPEB010000000
VERSION     DAZPEB010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4436
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.243"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..1088
                     /gene="nadB"
                     /locus_tag="ACHURJ_003813"
     CDS             <1..1088
                     /gene="nadB"
                     /locus_tag="ACHURJ_003813"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFR1282571.1"
                     /translation="VGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPDISSGD
                     GIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTRFMPDF
                     DERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLGLGIDL
                     TQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSLLECLV
                     YGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDYVGIVR
                     TTKRLERALRRINMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRKESRGL
                     HFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(1073..1810)
                     /gene="trmN"
                     /locus_tag="ACHURJ_003814"
     CDS             complement(1073..1810)
                     /gene="trmN"
                     /locus_tag="ACHURJ_003814"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFR1282572.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWLTQQTVRFDLIISNPPYYQQGVECAIPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            1942..3276
                     /gene="srmB"
                     /locus_tag="ACHURJ_003815"
     CDS             1942..3276
                     /gene="srmB"
                     /locus_tag="ACHURJ_003815"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFR1282573.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIEELRPKTRTPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(3485..4366)
                     /locus_tag="ACHURJ_003816"
     CDS             complement(3485..4366)
                     /locus_tag="ACHURJ_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1282574.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKGSDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCIEECFLPG"
BASE COUNT         1125 a         1209 c         1125 g          977 t
ORIGIN      
        1 ttgtaggcgc gtgggtatgg aaccgaaata aagaaacggt ggaaacctgc cacgcaaaag
       61 cagtggtact ggcaaccggc ggtgcgtcga aggtgtatca gtacaccacc aatccggata
      121 tttcttctgg cgatggcatt gctatggcgt ggcgcgcggg ctgtcgggtt gccaatctcg
      181 aatttaatca gttccaccct accgcactgt atcacccaca ggcacgcaat ttcctgttaa
      241 cggaagcact gcgcggcgaa ggcgcttatc tcaagcgccc ggatggcacg cgttttatgc
      301 ccgattttga tgagcgcggc gaactggccc cgcgcgatat tgtcgcccgc gccattgacc
      361 atgaaatgaa acgcctcggc gcagattgta tgttcctcga catcagccat aagcccgccg
      421 attttattcg ccagcatttc ccgatgattt atgaaaaatt gctcgggctg gggattgatc
      481 tcacacaaga accggtaccg attgtgcctg ctgcacacta tacctgcggt ggtgtaatgg
      541 ttgatgatca tgggcgtacg gacgtcgagg gcttgtatgc cattggcgag gtgagttata
      601 ccggcttaca cggcgctaac cgcatggcct cgaattcatt gctggagtgt ctggtctatg
      661 gctggtcggc ggcagaagat atcaccagac gtatgcctga tgcccacggc gtcagtacgt
      721 taccaccgtg ggatgaaagc cgcgttgaga accctgacga acgggtagta attcagcata
      781 actggcacga gctacggctg tttatgtggg attacgtcgg cattgtgcgc acaacaaaac
      841 gtcttgagcg cgccctgcgg cgtatcaata tgttgcaaca ggaaatagac gaatattacg
      901 cccacttccg cgtgtcgaat aatttgctgg agctgcgtaa tctggtgcag gttgcggaat
      961 tgattgttcg ctgtgccatg atgcgcaagg agagtcgtgg cctgcatttc acactggatt
     1021 atccggaact gctcacccat tccggtccgt cgatcctttc ccccggcaat cattacataa
     1081 acagataaaa agcctgggtc agcgccgtat acgcttcgga atagttctgg tctggcccac
     1141 gaatgactaa gcgatcgcta aagcattctc ccgcctgcgg ggagaatgcc agcagcaccc
     1201 gatgcggcag tcgcgcttcg ttttccgcca catccgtccg caaacgtaaa tgccagccca
     1261 tgcttaatgc cagctccgta aaaccattac caatctgctc tggcagcact acgcagaaaa
     1321 atccctcttc ggtaatgcac tccgccgcac aggtcagcag cgatgggtga tcaagcgtag
     1381 tggtatagcg agcctgttcc cgttgaggga tcgcgcactc tactccctgc tgatagtaag
     1441 gtgggttact gatgattaaa tcgaagcgta ctgtctgctg tgtgagccac tgctgaatat
     1501 ccgccgtatg gacgttaatc cgctctgccc acggggactg gttgatattt tcctgcgcct
     1561 gcgcggcagc ttcactttcc agctcaacgg catcaattat cacgctgtca ctggttcgct
     1621 gcgccagcat cagtgccagc aacccgctac ccgcgccgat atcaaggcaa cgtttcaccc
     1681 cagccaccgg tgcccatgcg cccagcaaaa tgccatccgt tcccactttc atcgcacagc
     1741 gatcgtgagc aacaaaaaac tgtttaaaag taaatccatt acgacgaagc acggatgtag
     1801 actgtgacat gaaaataaaa ccttgcagga aaaacggcga tagcaccggg tgagaacaat
     1861 acccgagaag cgatatccat acaaacagat gaagattgca gccgtaacgt ctataatcag
     1921 cgccccacac agaggtagaa catgactgta acgacttttt ccgaacttga actcgacgaa
     1981 agcctgctgg aagccctcca ggataaaggt ttcactcgcc cgaccgccat tcaggctgcc
     2041 gccattccgc ctgcgctcga tggccgtgat gtactcggtt ctgcgccgac aggcaccggt
     2101 aaaacggcgg cgtatctgct gccagcgttg cagcacctgc tcgatttccc gcgtaagaaa
     2161 tccggtccgc cgcgtatttt gatcctcacc ccaactcgcg agctggcgat gcaggtggcc
     2221 gatcatgccc gcgaactggc gaaacatacg catctggata tcgccaccat taccggcggc
     2281 gtagcctata tgaaccacgc ggaagtgttc agcgaaaatc aggacatcgt ggtcgccacg
     2341 accggacgtc tgctgcaata cataaaagaa gagaacttcg attgccgcgc ggttgaaacg
     2401 ctgatcctcg acgaagcaga ccgtatgctg gatatgggct tcgctcagga tatcgaacat
     2461 attgctggcg aaacgcgctg gcgtaaacag accctgctct tttcggcaac gctggaaggc
     2521 gatgcgattc aggactttgc cgagcgtctg ctggaagatc cggtggaggt ttctgccaat
     2581 ccgtccaccc gtgagcgcaa aaaaattcat cagtggtatt accgcgccga tgacctggaa
     2641 cataaaaccg cattgctggt gcatctgtta aaacagccgg aagcgacccg ctcaattgtg
     2701 tttgtgcgta agcgtgaacg tgtgcatgag ctggcaaact ggctgcgcga agcgggcatc
     2761 aacaactgct atctcgaagg tgagatggta cagggcaagc gtaatgaagc gattaaacgc
     2821 ctgaccgaag gccgcgtaaa cgtactggta gcaaccgatg ttgccgcgcg cggtatcgac
     2881 attccagacg tcagccacgt ctttaacttc gatatgccgc gcagtggtga tacttatttg
     2941 caccgtatcg gacgtaccgc gcgcgccggt cgtaaaggca ccgcgatttc gctggtggaa
     3001 gcccatgacc atctgctgct tggcaaagtt ggccgttata ttgaagagcc gataaaagct
     3061 cgcgtcattg aggaactacg cccgaaaacg cgtacgccaa gcgagaagca gaccggcaag
     3121 ccatcgaaga aagtactggc aaaacgcgcc gagaagaaaa aagctaaaga gaaagagaag
     3181 ccgcgggtga aaaaacgcca tcgcgacacc aaaaatattg gtaagcgccg taaaccgagc
     3241 ggaacgggcg tgccaccgca aacgacagaa gagtaatctt aattccgggt ttaagcccgg
     3301 aattaaaagt gctggcaaac gcaaaactgc ctgatgcgct acgcttatca ggcctacatg
     3361 gctcatgcaa tatattgaat ttgcacgatc ttgtaggccg gataaggcgt tcacgccgca
     3421 tccggcatga acaaaacgca cttcgtcgac aagttgggtg ccgggtttaa gcccggcatt
     3481 atttttatcc cggcaaaaaa cactcttcaa tacactgaat aaaagtgtgc atcgccgggc
     3541 taaccgcttt tccagcatgg tgtgcgcaca tagccgtaat ggtctgcgac tgttcgccaa
     3601 aaggcaattc aattaattct ccgctctcaa gctctttttc caccgcaaag cggggcaagt
     3661 aactgacacc gatattcgcc gcgacacaac gtttgatact ttcaatgctt aacagctcaa
     3721 tggtgttttc caccgtgatc cgccgctgac gcagcgtgct ctcaaatatc tgccggaaga
     3781 cacattgcgg ttcgttgata ataaagctac aggcgttatg tcttcccggc tcagtaaaat
     3841 cgacatctgc aatttgcggt gaagccacca gcaccagcga ttgttcaccc aactctcgtc
     3901 ggttcagggc atcatcattc cctacgcgat aaaagacgcc gacatcggcc tcatcattca
     3961 gtagcgcatc acggatcacg taacagttca gcgactgcaa cgataaacgc acttttggtg
     4021 cgcgttgccg aaaacgctgc aacacctgcg gcattcggta agagagcaac gtttcgcccg
     4081 aaacaacgcg tagttccccg tccggatctg accccttttt ggcagcctca cgaagcgtat
     4141 ccatcactcg ggtaagttca taaatgtgcg gcagcaactt ttttccttcg cgggtaaggc
     4201 acatccgtcg gccaattttc tcaaataact ggactgaaaa ctcctgctca agctgctgaa
     4261 tatgaaaagt caccgtcgat tgtgtacagc acaatttttg cgaagctcgc aaaaaggaac
     4321 cctcttccac cacggtttta agcgtaataa aacggcgcag atccataacc ccgaacctat
     4381 cgaaaatatc gaatctagaa tataaaaaca ttcatttttt taaatgttct gtgtcg
//
LOCUS       DAZPEB010000239         4418 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZPEB010000239 DAZPEB010000000
VERSION     DAZPEB010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4418
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.244"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            207..1064
                     /gene="focA"
                     /locus_tag="ACHURJ_003817"
     CDS             207..1064
                     /gene="focA"
                     /locus_tag="ACHURJ_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFR1282575.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            1119..3401
                     /gene="pflB"
                     /locus_tag="ACHURJ_003818"
     CDS             1119..3401
                     /gene="pflB"
                     /locus_tag="ACHURJ_003818"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFR1282576.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            3593..4333
                     /gene="pflA"
                     /locus_tag="ACHURJ_003819"
     CDS             3593..4333
                     /gene="pflA"
                     /locus_tag="ACHURJ_003819"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFR1282577.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
BASE COUNT         1123 a         1094 c         1076 g         1125 t
ORIGIN      
        1 ttagcggagg atgcggaaaa aattcaactc atttgttaat ttttaaaatt tatttttatt
       61 tggataatca aatatttact ccgtatttgc ataaaaacca tgcgagttac ggacctataa
      121 gccaggcgag atatgatcta tatcaatttc tcatctataa tgctttgtta gtatctcgtc
      181 gccgacttaa taaagagaga gttagtgtga aagctgacaa cccttttgat cttttacttc
      241 ctgctgcaat ggccaaagtg gccgaagagg cgggtgtcta taaagcaacg aaacatccgc
      301 ttaagacttt ctatctggcg attaccgccg gtgttttcat ctcaatcgca ttcgtcttct
      361 atatcacagc aaccactggc acaggcacaa tgcccttcgg catggcaaaa ctggttggcg
      421 gcatttgctt ctctctgggg ctgattcttt gtgttgtctg cggagctgat ctctttactt
      481 ccaccgtgtt gattgttgtt gctaaggcga gtgggcgcat cacctggggt cagttggcga
      541 aaaactggct aaatgtctat tttggcaacc tggtcggcgc actgctgttt gtacttttaa
      601 tgtggctttc cggcgagtat atgaccgcaa atggtcaatg gggactaaac gtcctacaaa
      661 ccgccgacca caaagtgcac catactttta ttgaggccgt ctgtcttggt atcctggcaa
      721 acctgatggt atgtctggca gtatggatga gttattctgg ccgcagcctg atggacaaag
      781 cgttcattat ggtgctgccg gtcgcgatgt ttgttgccag cggttttgag cacagtatcg
      841 caaacatgtt tatgatcccg atgggtattg taatccgcga cttcgcatct ccggaattct
      901 ggaccgctgt cggttctgca ccggaaaatt tttctcacct gaccgtgatg aacttcatca
      961 ctgataacct gatcccggtt acgatcggta atattatcgg cggtggtttg ttggttgggt
     1021 tgacatactg ggtcatttac ctgcgtgaaa acgatcacca ttaatggttg tcgaagtacg
     1081 cagtaaataa aaaatccact taagaaggta ggtgttacat gtccgagctt aatgaaaagt
     1141 tagccacagc ctgggaaggt tttaccaaag gtgactggca gaatgaagta aacgtccgtg
     1201 acttcattca gaaaaactac actccgtacg agggtgacga gtccttccta gctggcgcta
     1261 ctgaagcgac caccaccctg tgggacaaag taatggaagg cgttaaactg gaaaaccgca
     1321 ctcacgcgcc agttgacttt gacaccgctg ttgcttccac catcacctct cacgacgctg
     1381 gctacatcaa caagcagctt gagaaaatcg ttggtctgca gactgaagct ccgctgaaac
     1441 gtgctcttat cccgttcggt ggtatcaaaa tgatcgaagg ttcctgcaaa gcgtacaacc
     1501 gcgaactgga tccgatgatc aaaaaaatct tcactgaata ccgtaaaact cacaaccagg
     1561 gcgtgttcga cgtttacact ccggacatcc tgcgttgccg taaatctggt gttctgaccg
     1621 gtctgccaga tgcatatggc cgtggccgta tcatcggtga ctaccgtcgc gttgcgctgt
     1681 acggtatcga ctacctgatg aaagacaaac tggcacagtt cacttctctg caggctgatc
     1741 tggaaaacgg cgtaaacctg gaacagacta tccgtctgcg cgaagaaatc gctgaacagc
     1801 accgcgctct gggtcagatg aaagaaatgg ctgcgaaata cggctacgac atctctggtc
     1861 cggctaccaa cgctcaggaa gctatccagt ggacttactt cggctacctg gctgctgtta
     1921 agtctcagaa cggtgctgca atgtccttcg gtcgtacctc caccttcctg gatgtgtaca
     1981 tcgaacgtga cctgaaagct ggcaagatca ccgaacaaga agcgcaggaa atggttgacc
     2041 acctggtcat gaaactgcgt atggttcgct tcctgcgtac tccggaatac gatgaactgt
     2101 tctctggcga cccgatctgg gcaaccgaat ctatcggtgg tatgggcctc gacggtcgta
     2161 ccctggttac caaaaacagc ttccgtttcc tgaacaccct gtacaccatg ggtccgtctc
     2221 cggaaccgaa catgaccatt ctgtggtctg aaaaactgcc gctgaacttc aagaaattcg
     2281 ccgctaaagt gtccatcgac acctcttctc tgcagtatga gaacgatgac ctgatgcgtc
     2341 cggacttcaa caacgatgac tatgctatcg cttgctgcgt aagcccgatg atcgttggta
     2401 aacaaatgca gttcttcggt gcgcgtgcaa acctggcgaa aaccatgctg tacgcaatca
     2461 acggcggcgt tgacgaaaaa ctgaaaatgc aggttggtcc gaagtctgaa ccgatcaaag
     2521 gcgatctcct gaactacgat gaagtgatgg agcgcatgga tcacttcatg gactggctgg
     2581 ctaaacagta catcactgca ctgaacatca tccactacat gcacgacaag tacagctacg
     2641 aagcctctct gatggcgctg cacgaccgtg acgttatccg caccatggcg tgtggtatcg
     2701 ctggtctgtc cgttgctgct gactccctgt ctgcaatcaa atatgcgaaa gttaaaccga
     2761 ttcgtgacga agacggtctg gctatcgact tcgaaatcga aggcgaatac ccgcagtttg
     2821 gtaacaatga tccgcgtgta gatgacctgg ctgttgacct ggtagaacgt ttcatgaaga
     2881 aaattcagaa actgcacacc taccgtgacg ctatcccgac tcagtctgtt ctgaccatca
     2941 cttctaacgt tgtgtatggt aagaaaactg gtaacacccc agacggtcgt cgtgctggcg
     3001 cgccgttcgg accgggtgct aacccgatgc acggtcgtga ccagaaaggt gctgtagcgt
     3061 ctctgacttc cgttgctaaa ctgccgtttg cttacgctaa agatggtatc tcctacacct
     3121 tctctatcgt tccgaacgca ctgggtaaag acgacgaagt tcgtaagacc aacctggctg
     3181 gtctgatgga tggttacttc caccacgaag catccatcga aggtggtcag cacctgaacg
     3241 ttaacgtgat gaaccgtgaa atgctgctcg acgcgatgga aaacccggaa aaatatccgc
     3301 agctgaccat ccgtgtatct ggctacgcag tacgtttcaa ctcgctgact aaagaacagc
     3361 agcaggacgt tattactcgt accttcactc aatctatgta attagatttg actgaaatcg
     3421 tacagtaaaa agcgtacaat aaaggctcca cgaaagtggg gcctttttta gcacgagagc
     3481 cttttttgtc agctatctat actttaaggt gactgccaaa acagactcga cgtagccttc
     3541 gagctgcgca ccaacacggc ctcagatggg ccacatctgg agaaacaccg caatgtcagt
     3601 tattggtcgc attcactcct ttgaatcctg tggaaccgta gacggcccgg gtattcgctt
     3661 tatcaccttt ttccagggct gcctgatgcg ctgcctgtat tgtcataacc gcgacacctg
     3721 ggacacgcat ggcggtaaag aagttaccgt tgaagatttg atgaaggaag tggtgaccta
     3781 tcgccacttt atgaacgctt ccggcggcgg cgttaccgca tccggcggtg aagcaatcct
     3841 gcaagctgag tttgttcgtg actggttccg cgcctgcaaa aaagaaggca ttcatacctg
     3901 tctggacacc aacggttttg ttcgtcgtta cgatccggtg attgatgaac tgctggaagt
     3961 aaccgacctg gtaatgctcg atctcaaaca gatgaacgac gagatccacc aaaatctggt
     4021 tggagtttcc aaccaccgca cgctggagtt cgccaaatat ctggcgaaca aaaatgtgaa
     4081 ggtgtggatc cgctacgttg ttgtcccagg ctggtctgac gatgacgatt cagcgcatcg
     4141 cctcggtgaa tttacccgtg atatgggcaa cgttgagaaa atcgaactcc tcccctacca
     4201 cgagctgggc aaacacaaat gggtggcaat gggtgaagag tacaaactcg acggtgttaa
     4261 accacctaag aaagagacca tggaacgcgt gaaaggcatt cttgagcagt acggtcataa
     4321 ggtaatgttc taatttcccc gtaaagcggc aactttattg agttgccgct tttttattcc
     4381 gcgcttcatt ctgctacagt gtttttaaca cattttat
//
LOCUS       DAZPEB010000240         4399 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZPEB010000240 DAZPEB010000000
VERSION     DAZPEB010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4399
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.245"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..368
                     /locus_tag="ACHURJ_003820"
     CDS             <1..368
                     /locus_tag="ACHURJ_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFR1282578.1"
                     /translation="IAEWPGEGGEGHLSSFFVQTDKASINVAYHHTVNQQKELGTFGA
                     FDHDWHTLAFKFKGSNSINVTPVLDGVDGQAFDLVKWANTANGLNRFVITDITGSAET
                     YPVLIDTVEVKANKAGAAA"
     gene            508..690
                     /locus_tag="ACHURJ_003821"
     CDS             508..690
                     /locus_tag="ACHURJ_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFR1282579.1"
                     /translation="MTFIHQVMLYFCTAVCVLYLLSGGYRAVRDFWCRQIDKRAAEKI
                     SASQSAGSKPEEPVTP"
     gene            614..961
                     /locus_tag="ACHURJ_003822"
     CDS             614..961
                     /locus_tag="ACHURJ_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001687362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFR1282580.1"
                     /translation="MIKGPLRKSAPVSQPEANPKNPLLLNNPFQRENPMSEITSLVTA
                     EAVKEVLRSEEVRSALKQKLRQNLESRLDAEVDAILDELLGVAAVPEPEGIAGDGSAS
                     DGGEPTPDSDMMM"
     gene            1038..1253
                     /locus_tag="ACHURJ_003823"
     CDS             1038..1253
                     /locus_tag="ACHURJ_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFR1282581.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRFSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            1258..1602
                     /locus_tag="ACHURJ_003824"
     CDS             1258..1602
                     /locus_tag="ACHURJ_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HFR1282582.1"
                     /translation="MKKKYELVVKGINNYPNKITVTVALEIGGYPSLLLPDVAISLDR
                     TEDATLEFYEAEAKKQAKQFFMDVAAGLCEGDGPLPEKRPVILEAQDVLITYRGKLPG
                     IITGSLKTPPLA"
     gene            1653..2186
                     /locus_tag="ACHURJ_003825"
     CDS             1653..2186
                     /locus_tag="ACHURJ_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFR1282583.1"
                     /translation="MNTKIKYGLSAAVLALIAAGAPAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDSKPVVPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWDIDR"
     gene            2342..2524
                     /locus_tag="ACHURJ_003826"
     CDS             2342..2524
                     /locus_tag="ACHURJ_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310767.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282584.1"
                     /translation="MAKYKRILLRLTMKNGLELKAPVTDDISRALAFAIKWVAVGVAV
                     SPMLYGLAKLVIALKS"
     gene            2539..2670
                     /locus_tag="ACHURJ_003827"
     CDS             2539..2670
                     /locus_tag="ACHURJ_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282585.1"
                     /translation="MSDKLITLAKILCVIVGISFSLMLVALFLSMAWMMLSSSGLLG"
     gene            2851..2940
                     /locus_tag="ACHURJ_003828"
                     /pseudo
     CDS             2851..2940
                     /locus_tag="ACHURJ_003828"
                     /inference="COORDINATES: protein motif:HMM:NF017860.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2904..3065
                     /locus_tag="ACHURJ_003829"
     CDS             2904..3065
                     /locus_tag="ACHURJ_003829"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
                     /protein_id="HFR1282586.1"
                     /translation="MREATGTSGMDNATGPRLADTAERDYFTLRERLMTMQKQLEGAQ
                     EYIRTQCIN"
     gene            3089..3313
                     /locus_tag="ACHURJ_003830"
     CDS             3089..3313
                     /locus_tag="ACHURJ_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282587.1"
                     /translation="MKKLLVTVKPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVSAALQPVC"
     gene            3658..3984
                     /locus_tag="ACHURJ_003831"
     CDS             3658..3984
                     /locus_tag="ACHURJ_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HFR1282588.1"
                     /translation="MKRNLPLIILLSSLVMGCTQHKTDMPRQLVKALPQYPAYAAANY
                     IKGRVDVKFDIGADGTVTRIEFIRSEPHHLFDEQVVKAMAKWRFEKDRPCKGVKKTFI
                     FSPSAP"
     gene            complement(4116..4316)
                     /locus_tag="ACHURJ_003832"
     CDS             complement(4116..4316)
                     /locus_tag="ACHURJ_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFR1282589.1"
                     /translation="MSSKNRTRRTTTRNIRFPNQMIEQINIALDLKGSGNFSAWVIEA
                     CRRRLATDATHLRPASMKNNEK"
BASE COUNT         1125 a          938 c         1212 g         1124 t
ORIGIN      
        1 gaatagcgga atggccgggt gagggtggtg aaggccatct gagttctttc tttgtccaga
       61 cagataaagc cagtattaat gttgcatacc atcatacagt taatcaacaa aaagaacttg
      121 gcacgtttgg cgcattcgac catgactggc atacgcttgc atttaaattt aagggcagta
      181 acagcattaa tgttactccg gtgcttgatg gtgtggatgg acaggcgttt gacctggtga
      241 aatgggccaa tactgctaat ggactcaaca ggtttgtcat tacggatatt acaggtagtg
      301 cagaaaccta ccctgtactt attgatacgg tggaagttaa agcaaacaaa gctggagcag
      361 ccgcataatt gctaaaaaaa gccgccagcg gcaggaatgg aagctggcgg aggtaatccc
      421 aatggagaat gtaaagaaaa gatgctttcg tacattggtt ttttaaatga aaacaattct
      481 cattgtcaac cataacggta agaaattatg acatttattc atcaggtgat gctgtacttc
      541 tgtacggcgg tctgtgtgct gtatcttctt tcgggtgggt acagggctgt gcgcgatttc
      601 tggtgcaggc agattgataa aagggccgct gagaaaatca gcgccagtca gtcagccgga
      661 agcaaacccg aagaacccgt tactccttaa caaccccttt cagcgagaaa atcccatgtc
      721 agaaatcaca tccctggtca ctgcagaggc agtgaaggaa gtcctgcgct ctgaagaagt
      781 ccggagcgca ctgaaacaga aacttcgcca gaatcttgag tcgcgtcttg atgcagaagt
      841 ggatgccatt ctggatgagc tgctgggcgt agcagcggtt cctgagccgg aaggtatcgc
      901 gggtgacggg agtgcttcag atggcggtga acctacacct gacagcgaca tgatgatgta
      961 agcatgcgca agggaccatc ggtgtgtgcc ggtggtcttt atattgttgt gagcttccgg
     1021 attgcgggag acggggtatg taccagatgg aaaaaatcac aacaggtgtg tcatacacca
     1081 cgtcagcggt gggaacgggc tactggttcc tgcagttgct ggacaggttt tccccgtctc
     1141 agtgggcggc aataggcgtg ctggggagtc tgttgtttgg gctgctgaca tatctgacta
     1201 acctgtattt caaaatcaga gaggaccgtc gtaaggcggc gcggggagag taaagcgatg
     1261 aagaaaaaat acgaactggt tgttaaaggg ataaataatt acccgaataa gattactgtt
     1321 actgtggcac tggaaattgg tgggtatccg tcactgttgt tgccagatgt ggcgattagt
     1381 cttgaccgta ctgaagatgc cacgctggag ttttacgaag ctgaggcgaa aaagcaggcg
     1441 aagcagtttt tcatggatgt tgctgccggg ttatgtgaag gggatggtcc gttgccggaa
     1501 aagcgccccg taattttaga ggcgcaggat gtgttgataa cctacagagg aaaactaccg
     1561 ggaataatta ctggttctct gaagactcca ccgctggcct gaagacttaa catatccagg
     1621 gatttgaaat cgataaaccc tgataaatat ccatgaacac caaaatcaaa tacggcctgt
     1681 cggctgccgt tctggcgctg attgccgcag gtgcgcctgc gcctgaaatc ctcgaccagt
     1741 ttctggatga aaaggaaggt aaccacacca cggcataccg tgatggcgcg ggtatctgga
     1801 ccatctgccg cggtgccatc ctggtggata gtaaacctgt cgtcccgggc atgaagttgt
     1861 cgaaggaaaa atgcgaccag gtcaacgcca ttgagcgtga taaggcgctg gcatgggtgg
     1921 agcgcaatat taaagtgccg ctgaccgaac cccagaaagc gggtatcgcg tcattttgtc
     1981 cctataacat tggccccggt aagtgtttcc cgtcgacgtt ttacagacgg attaatgctg
     2041 gtgaccgcag gggagcatgc gaagcgattc gctggtggat taaggacggt ggcagagact
     2101 gccgtatccg ctcaaataat tgctacggtc aggtatcccg gcgagaccag gaaagtgcgc
     2161 tggcgtgctg ggacatcgac agataagcag aatattttgc tgaaaataag gcatgagcac
     2221 gcggacggat aacacgaaat cctgcgaact ggcgaaacgt aagtgaataa aagtaaaaac
     2281 cccgtttgtt ggcaccaggc ggggttttgt gtttctgacc ttgagtaagg caagggagaa
     2341 catggcgaag tataaacgaa ttctgttgag gttgaccatg aaaaacggcc ttgaactgaa
     2401 agcgcctgta actgatgaca tcagcagagc actggctttt gccattaagt gggtggcggt
     2461 cggtgttgct gtgtccccga tgctgtatgg gctggcaaaa ctggtcattg cgttgaaatc
     2521 gtgaagggag gattaagcat gtcagacaaa ctcataacgc tggcgaagat cctctgtgta
     2581 attgtcggca tttcattttc actaatgctg gttgctcttt ttctttccat ggcctggatg
     2641 atgttgtctt cgtcggggct gctggggtga acataaaccg aatgctttcc gcgtttaccg
     2701 ttattctgct ggtggtctgt ggtgcgctgt ggctggcaac agaccattac cgtgataacg
     2761 ccatcaccta caaagcgcag cgcgataaaa aagccagaga gctggagctg gcaaacgcaa
     2821 ccattactga catgcagcag cgccagcgtg atgttgccgc tggtcgtaag cgcctgcgga
     2881 tcaacgccac ctgctccggt accgtgcgtg aagccaccgg cacctccgga atggataatg
     2941 caaccggccc ccgactggca gacaccgctg aacgggatta tttcaccctc agagagcggt
     3001 tgatgacaat gcagaagcaa ctggaagggg cgcaggaata tatccgcact cagtgtatta
     3061 actagtattt ttgttatccg gagaatgcat gaagaaatta ctggtaaccg taaagccttt
     3121 tcagggaaca attccgttcc gtattttgca gcgtggtcgt gttcttgttg aaggttcgtt
     3181 cagtggtaaa tgtacgcaat tacactcccg gacctttcag gtgaatgcca cgaatgaaga
     3241 gctaaccgtt gagtgtacga tgaatgccgc taaatgccgc atggtatccg ctgcattaca
     3301 gccagtgtgt tgagcgacct tattaaccat gcgcggtatt gtcgccgtat ccccgcatta
     3361 acagagaccg cagcccgacc gggagactcc tctgcgcgag tgtgcgggga taatcaaaaa
     3421 cgatacacac cggggtttac cgcgttaacg gagcgcggcg ttgtcccctc atggtcgctg
     3481 gtccggtgcg atggtggaag aaaccggacg atgtgttacc tcgcaagctc tgttatgtca
     3541 tgtgtctgat ttgtgattta agtcggataa ttgtcgttgc cattaagcag aggattgatg
     3601 accgacaggg tggcattgtt agaataagac ttattcttat ctgtgccggg aatgaaaatg
     3661 aaaagaaacc ttccgttaat tattttgttg tcttctctgg ttatgggctg tacgcaacat
     3721 aaaacagata tgccccgaca gttggttaag gcattaccac aatatccggc ctatgcagcg
     3781 gcaaattata taaagggacg ggttgatgtg aagtttgata ttggtgctga tggtactgtc
     3841 acccgaattg agtttatccg ttcagagccg caccatttgt ttgatgagca ggttgtaaag
     3901 gcgatggcaa aatggcgatt tgagaaggac aggccgtgta aaggcgtgaa gaaaacgttt
     3961 atctttagtc cttctgcacc ctgattattt catcagaaat taattatcac tctgttgtta
     4021 ttctgtacat cccggcaggg taagtcttgt tccgtcggat atgaagatga aatattgttg
     4081 gaggacagtg ggtacctgct cctgtaaccg aacgttcatt tctcgttatt tttcatgctg
     4141 gccgggcgca gatgcgttgc atctgttgcc agccttctcc tgcaggcttc aataacccac
     4201 gctgaaaagt ttcctgaacc tttcagatca agagcgatgt taatttgttc aatcatctgg
     4261 tttggaaatc ggatgttgcg ggttgttgtt ctgcgggttc tgttctttga tgacataatg
     4321 tttccccata ttcagtgttg ctgatttgta ttatctgaag ttgcttttac gttaatttga
     4381 tgcagatcaa tcaatacga
//
LOCUS       DAZPEB010000241         4380 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZPEB010000241 DAZPEB010000000
VERSION     DAZPEB010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4380
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.246"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(62..724)
                     /gene="exoX"
                     /locus_tag="ACHURJ_003833"
     CDS             complement(62..724)
                     /gene="exoX"
                     /locus_tag="ACHURJ_003833"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFR1282590.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWSFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(748..1404)
                     /gene="yobB"
                     /locus_tag="ACHURJ_003834"
     CDS             complement(748..1404)
                     /gene="yobB"
                     /locus_tag="ACHURJ_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFR1282591.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLACDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            complement(1506..1736)
                     /gene="holE"
                     /locus_tag="ACHURJ_003835"
     CDS             complement(1506..1736)
                     /gene="holE"
                     /locus_tag="ACHURJ_003835"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFR1282592.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVKLSRLPYEPKLK"
     gene            1875..2249
                     /gene="yobA"
                     /locus_tag="ACHURJ_003836"
     CDS             1875..2249
                     /gene="yobA"
                     /locus_tag="ACHURJ_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFR1282593.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            2253..3120
                     /gene="copD"
                     /locus_tag="ACHURJ_003837"
                     /pseudo
     CDS             2253..3120
                     /gene="copD"
                     /locus_tag="ACHURJ_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            3133..3474
                     /locus_tag="ACHURJ_003838"
     CDS             3133..3474
                     /locus_tag="ACHURJ_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFR1282594.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(3856..>4380)
                     /locus_tag="ACHURJ_003839"
     CDS             complement(3856..>4380)
                     /locus_tag="ACHURJ_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1282595.1"
                     /translation="YNCIREAADQLPAWFPLAMDLALVTGQRREDITNMRFSDIYDDR
                     LHIRQIKTGMMIAIPLSLSLPVAGLRLGAVVEQCRMVSRGDYLISAGIRKNSPDGSIH
                     PDGLTKKFVAARKLTGIQFSENPPTFHEVRSLAGRLYKETCGEEFAQRLLGHTSEKTT
                     KMYLDEREKTYLLL"
BASE COUNT         1091 a         1057 c         1079 g         1153 t
ORIGIN      
        1 cgatggaata tgcttttctg gtcgcgctgg cgcagggaac attacccgct acgcctgcgg
       61 attaagtatt ttccagataa tgtttcagtg ttaaacgcag ctccgggctc atgctgtcca
      121 ggttattaaa tgaccagcgc agatagcccg gatcgcgttc ggcaacgtcg gaaaccgctt
      181 tgccacggta tttgccaaag gtgaaggtcg tcatcaacga cggacgtccg gtgatatcgg
      241 ccatctgttc tgccgtccag ccggaggtgt tcataatatc gataagcaac gcggcggtga
      301 tataacagtc atacaacgcg cggtgatgat gcaggcccgg cggcgtctgt acattgagct
      361 tccgtgtttt atataacgcc atattgctgt acttgatccc tggccacaaa cgacgggcca
      421 gtttcatagt gcaaatccac tcaccgggca tctcaggcag tacgcggcgg tcaaagctgg
      481 cgttgtgcgc gacataccat tcactaccgt aatagtgtgg gatcacatct tcaatccacg
      541 gtttatcggc gaccatggct tcggtgatgc gatgaatcgc catcgcttgt ggactaatag
      601 gacgatcggg gcgcaccagg tggctcatgg ggttgacgat ttttccgtca atgacatcaa
      661 cagaggcaat ctcaacgatc cctccctgca aaccgcaggt ttctgtatcg ataatgcgca
      721 acatggcatg ctccaggccg aaaaagccta gcgtaatgga atgatatcgc cttgccaacc
      781 ctgtgatgaa cgctgaccga ctaacaataa tgaaccacga tcggcgcgaa cgatgagacg
      841 accatattca tcccatagtg cactgttacc acgggcattt gccatcagca cggcaatcga
      901 gtactgatgt gaaaaaaatt gtaagcgacg ggccgacgcc agcaggtcgg gttctcccag
      961 acattgccct gtagtaaaca gtgaacaggt cgggtccata tccatacctt gcggttgttc
     1021 atcgaccacc gtgatggttc tggaacgacg gcccagacat gcaccatgac tctgatggta
     1081 gatcccaggc gtttttcgcc acggtgcgaa caccgcaatt ccacgaataa agcgatcgtt
     1141 atattcaacg ggaaggccgg caatgatggt cattcgccag gtcgttgcgg cataacaaag
     1201 cgggtctaac agtgagagat cgggtggggc aggaagggca cgtcgcgaat aatcacacgc
     1261 cagtaaagaa agcgatggaa aaactaacag ctgacattgt tgcctggcgg cggccctcac
     1321 aaactccaga tgatgagcta cctgttcggt gagtgaggtt ttacgaggct catattgcgc
     1381 tgctgcaact ttccagaacg acataatgac ttccttttca aaatgactgc aaattatggg
     1441 tgcggtttag aaaatactca taatttagca ggcgttatgt aagaaagtgt aactctgtat
     1501 aagttttatt taagtttggg ctcgtaaggt aaacgtgaca gtttgaccga agccaaacgg
     1561 tgggcaataa gcctctcgcg aaaccagctg cgcaaatgtt caggctgttc acgttcaacc
     1621 gcttcagcga tcaccggcat attgtagcgt tctttaaatg ccaccccggc cgccgccaaa
     1681 tcgacattca ctttatccat ttctgtttga tccagtttag ccagattctt cagcattctt
     1741 aatctcctca tcattcgcgg gcaaaagtta ctaagagtga gccaggatgg caagccggaa
     1801 ttgatgatcc tcgactcaga cattagacaa ggttatgctc tgtaaaaaag ttattacaaa
     1861 aagtaataga taatatggct tcaactgcac gctcccttcg ctacgcgctg gcaatcctga
     1921 ccacttcgtt ggtgacacct tccgtctggg cacatgcgca tttaacgcat cagtatcctg
     1981 cggcaaacgc gcaagtgaca gctgcaccgc aggcaatcac tttaaacttc tcggaaggtg
     2041 ttgaaacagg attcagtggt gcaaaaatca cggggccaaa aaacgaaaat attaaaacat
     2101 taccggcgaa gcgaaatgag caggaccaaa agcaactgat tgttccactg gccgattcgc
     2161 tgaaacccgg aacctacacc gttgactggc atgttgtttc ggtggacggg cataaaacga
     2221 aaggacacta cacctttagc gtgaaataaa tcatgctggc gtttacctgg atcgcgctgc
     2281 gatttatcca tttcacctcg ctgatgctgg tttttggctt cgcaatgtac ggtgcctggc
     2341 tggccccctt gacgattcgt cgcttgctgg cgaagcgttt tctgcgttta caacagcacg
     2401 ctgccgtctg gagtttgatc agtgccactg caatgctcgc cgttcagggc gggttgatgg
     2461 gaacgggatg gacagatgta ttttcgccaa acatttggca ggctgttttg caaacgcagt
     2521 ttggtggcgt ctggttatgg caaattgttc tcgccctcgt aacgttgcgc ccttatgcaa
     2581 ccgcggaata tgccacgttt gctgtttatg ctcactaccg cgcaatttat tctgctggcg
     2641 ggagtggggc atgcgacgct gaatgaaggg gtaacggcga aaatccatca gactaatcac
     2701 acgattcacc tgatttgtgc ggcggcgtgg tttgggggat tactgccggt gctctggtgt
     2761 atgcaactca tcaaaggccg ttggcgacac caggctattc aggcgctgat gcgtttttca
     2821 tggtgcgggc attttgcggt gatcggcgta ctggcaagcg gggtgcttag tgcattgttg
     2881 attaccggat ttccccccac gcttacgact tactggggcc aactcttatt gctcaaagcc
     2941 atcctggtga tgattatggt ggtcattgcg ctggctaatc gttatgttct tgtaccgcgc
     3001 atgaggcagg atgaagatcg tgcggcaccc tggtttgtgt ggatgactaa actcgaatgg
     3061 gcgataggtg cggtagtgct ggtgatcatc agcctgcttg cgactctcga acctttttga
     3121 tggattacga aaatgatgaa aaaaagtatt ctggcgtttc tgttactcac cagttctgca
     3181 gcggcgctgg ctgcaccgca ggtgattacc gtcagtcgtt ttgaagtggg taaagacaaa
     3241 tgggcgttta atcgcgaaga ggtgatgctg acttgccgac cgggtaatgc tttgtatgtt
     3301 attaatccga gtaccctcgt gcagtatcct ttaaacgata tcgcacaaaa ggaagttgcc
     3361 agtgggaaga ccaatgccca acccatttcg gtgattcaga ttgatgatcc taacaatccc
     3421 ggcgaaaaaa tgagtctggc accgtttata gaacgagctg aaaaactctg ttaattacct
     3481 aaaatagcct tttgatttcc aataaaaaaa ccgcctcagt tctttcacca gaacgggcgg
     3541 ttttaacatt tcagctgatg accaccacgc tttttattga ccattttgca cgcaaactgg
     3601 aaaacctggc gtcgtcatct attcttaaag ggcaaggcaa ctaagcctgc attaatgcca
     3661 acttttagcg cacggctctc tcccaagagc catttccctg gaccgaatac aggaatcgta
     3721 ttcggtcttt ttttaattgc atttaaaatc aattagttgc aaatgtctcc ccgaaattta
     3781 ctcgaatttc tgtattccgg tcttttttgg ttatatcaca accaaaatac atttaacaat
     3841 ccatttacgt taaaatcaga gcagtaagta cgttttttct ctctcatcaa gatacatttt
     3901 tgttgtcttc tccgatgtgt ggccaagtag acgctgagca aattcttctc cacatgtttc
     3961 tttgtacaat cgtccagcca ggcttctgac ttcgtgaaaa gttggtgggt tttcactgaa
     4021 ctggatacct gttaattttc tggctgcgac aaatttcttt gtcaggccgt ccgggtgaat
     4081 gctgccgtca gggctgtttt ttctaatccc ggcactgatt agataatccc ccctacttac
     4141 catgcggcac tgttcaacta ctgcaccaag tcgtaaacca gcgacaggaa ggctgagtga
     4201 cagggggata gcaatcatca ttcctgtctt aatttgcctg atgtggagac gatcatcata
     4261 aatatcactg aaccgcatat tcgttatgtc ttcgcgacgt tgtcctgtta caagggctaa
     4321 atccatagct aatgggaacc atgccggaag ttgatctgct gcctccctga tgcagttgta
//
LOCUS       DAZPEB010000242         4312 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZPEB010000242 DAZPEB010000000
VERSION     DAZPEB010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4312
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.247"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            380..988
                     /locus_tag="ACHURJ_003840"
     CDS             380..988
                     /locus_tag="ACHURJ_003840"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFR1282596.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSHESFARTEPPKA"
     gene            1086..2477
                     /gene="selA"
                     /locus_tag="ACHURJ_003841"
     CDS             1086..2477
                     /gene="selA"
                     /locus_tag="ACHURJ_003841"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFR1282597.1"
                     /translation="MTTETRSLYSQLPAIDRLLHDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2474..>4312
                     /gene="selB"
                     /locus_tag="ACHURJ_003842"
     CDS             2474..>4312
                     /gene="selB"
                     /locus_tag="ACHURJ_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFR1282598.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPSFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPGREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFSDEPWW
                     VRDLAKETGTDEQAMRLTLSQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPE"
BASE COUNT          975 a         1112 c         1252 g          973 t
ORIGIN      
        1 ggattgaccg gatggccgga cgtcactccg ccggggcaca ccgaacaggc cacgccggtg
       61 ggggtaccaa tgcgcacccc ggctgaaccc tgaacaatgc taccgccata ctgcgtcatg
      121 tcgccctgac gcgctgccgg ttttccgctc atttctcatc cttgttttat cgggttattt
      181 gtctgctgac aggcaaaacc ctacagattt ataagaaagc ctgaagtgag atatccatca
      241 gagtgaaata attaggaaaa tatttatatc accatgataa atatgtgatt tatttggaag
      301 taacaaacca tcaacatttt ctgccctttt tcccatcacc aggccatact gatatttttc
      361 ctcgcacaaa ggactcggca tgaaactcgt cggtagctac accagcccgt ttgtacgcaa
      421 actttctatt ctgttgttag aaaagggcat aactttcgaa tttattaatg aactgcccta
      481 taacgcggac aacggcgtgg cgcaatttaa cccgttagga aaagtgccgg tgctggtgac
      541 cgaagagggc gaatgctggt ttgattcgcc gatcatcgct gaatatattg agttaatgaa
      601 tgtcgctccg gcgatgttgc cgcgcgatcc gctggagtcg ttgcgggtgc gcaaaattga
      661 ggcgctggcg gatggcatta tggatgccgg gctggtatcg gtgcgtgaac aggcgcgtcc
      721 ggcggcgcag cagtctgaag atgaattgtt acgccagcgg gagaaaatca accgcagtct
      781 ggatgtgctg gaaggatatc tggtcgatgg cacactcaaa accgatacgg tcaatctggc
      841 gactatcgcc attgcctgtg ctgtcggata tctcaatttc cgccgcgttg cgccaggttg
      901 gtgtgtcgac cgcccacact tagtcaaact ggtcgaaaac ctgtttagcc acgaaagttt
      961 tgctcgcacc gaaccgccaa aggcttgatg cgcgatatgt cctcctgacc catctcacgt
     1021 tacaatccgt ggttatgtta aacgcccttc tccatgtgag agggccttga ttagccaggt
     1081 ttcctatgac aaccgaaacg cgttccctct atagtcaact tccggctatt gatcgcttat
     1141 tgcacgatag ctccttcctt tctttgcgtg atacttatgg tcacacccgc gtggtggaat
     1201 tgttgcgtca gatgctcgac gaagcgcgag aagtgattcg tgacagccag acgcttcctg
     1261 cgtggtgtga aaactgggcg caagaagtcg atgcccggtt gacgaaagag gcgcagagcg
     1321 cgctgcgtcc ggtgatcaac ctgacgggaa ccgtgctgca taccaacctt ggacgagctt
     1381 tacaggcgga agccgcggtg gaagccgttg cgcaggctat gcgttcgcca gtgaccctcg
     1441 agtacgatct ggacgacgct ggacgcggac atcgcgatcg ggcgctggcg caactgctgt
     1501 gccgtattac gggggcggaa gatgcctgta tcgtcaataa caatgcggcg gcggtgttat
     1561 tgatgttggc ggccactgcc agcgggaaag aggtggtagt atctcgcggc gaattggtgg
     1621 agattggcgg cgcgtttcgt attcccgatg ttatgcgtca ggcaggctgc accctacacg
     1681 aagtagggac caccaaccgc acgcacgcga atgattatcg tcaggcggtg aatgaaaata
     1741 ccgcactgtt gatgaaagta cataccagta actacagcat tcaggggttc accaaagcga
     1801 tagatgaagc ggaactggtg gcgctcggca aagagctgga tgttcccgta gtgactgatt
     1861 taggcagtgg ctcgctggtc gatcttagcc agtacggttt gccgaaagag ccaatgccgc
     1921 aggagttgat tgcggcgggc gtcagtctgg tgagcttctc cggcgacaag ttgttaggcg
     1981 ggccgcaggc agggattatc gttggtaaaa aagagatgat cgcccgcctg caaagccacc
     2041 cgctgaagcg tgcattacgc gcggataaaa tgaccctcgc ggcgctggaa gccacgttgc
     2101 gtctttattt acaccctgaa gctctgagtg aaaaattacc gaccctgcgc ctgcttaccc
     2161 gcagcgcaga ggtcattcaa atccaggcac aacgtttaca ggctcccctt gccgcacatt
     2221 acggcgcgga gtttgcggta caggttatgc catgtctttc gcagattggc agtggttcgc
     2281 tgccggttga tcgcctgccg agcgcggcat taacgtttac accccatgat ggacgcggta
     2341 gccaccttga gtcattagcc gcccgctggc gtgaattgcc agtgccggtg attggtcgta
     2401 tttatgacgg acgattgtgg ctggatttac gctgccttga agatgagcaa cggtttttgg
     2461 agatgttgtt gaaatgatta ttgcgactgc cggacacgtt gaccacggca aaacaacctt
     2521 attgcaggcg attactggcg taaatgctga ccgtctgccg gaagaaaaaa agcgcggcat
     2581 gaccatcgat ctcggctatg cctactggcc gcagccggat ggtcgcgtgc ctagttttat
     2641 cgacgtaccc ggtcatgaaa agtttctttc caacatgctg gcgggcgttg gtggtatcga
     2701 tcacgcgctg ttggtggtgg cgtgcgatga cggcgtgatg gcgcagaccc gtgagcatct
     2761 ggcgattttg cagctgaccg gtaacccgat gctgacagtg gcgctgacca aggccgatcg
     2821 cgtggacgaa gcgcgtgttg atgaggttga acgccaggta aaggaggtgc tgcgggaata
     2881 cggttttgct gaggcaaaac tgtttatcac cgcagcaacc gaaggtcggg gaattgatgc
     2941 cctgcgcgag catctgcttc agttaccagg acgtgaacac gccagccaac atagtttccg
     3001 cctcgcgatc gaccgcgcat ttaccgtaaa aggtgccggg ctggtcgtca ccggtacggc
     3061 gttaagcggg gaagtgaagg taggcgattc actctggctg actggtgtaa ataaaccgat
     3121 gcgtgtacgt gcgctgcatg cacaaaacca gccaacagaa accgcccatg ccgggcagcg
     3181 tatcgcgctt aacatcgcgg gtgatgcgga aaaagagcag attaaccgtg gcgactggct
     3241 gcttgccgat gcgccgccag agccgttcac acgggtgatt gtcgagcttc aaacccatac
     3301 accgctgacc cagtggcagc cgctgcatat tcaccacgcc gccagccacg tcacgggacg
     3361 cgtttcactg ctggaagata accttgctga actggtcttc gacaccccgt tatggctggc
     3421 agataacgac cgcctggtat tgcgcgatat ctctgcccgc aacacgctgg ccggagcgcg
     3481 cgtcgtgatg cttaacccgc cgcgtcgcgg taaacgtaaa ccagagtatc tgcaatggct
     3541 ggcttccctc gccagggcac agaatgatag cgaagcgtta gccgttcacc tgcaacgcgg
     3601 cgcggttaat cttcctgatt tcgcctgggc gcgccagctc aacggcgaag ggatgcgcga
     3661 actgctgcaa cagcctggct atattcaggc tggttatagc ttgttgaatg cgccggttgc
     3721 cgctcgctgg caacggaaaa ttctcgacac attagcgact tatcatgagc aacatcgcga
     3781 tgaacctggg cctgggcgcg aacgtctgcg acgtatggcg ttgccaatgg aagatgaagc
     3841 gctagtactg ttgctgattg aaaagatgcg cgaaagcggc gacatcctca gtcatcacgg
     3901 ttggctgcat ctgccggatc acaaagcggg cttcagcgaa gagcagcagg ccatctggca
     3961 aaaagcagag ccgctgttta gtgacgagcc gtggtgggtg cgtgatctgg caaaagagac
     4021 gggtaccgac gagcaggcaa tgcgcttgac gctaagccag gcggcgcagc aaggaataat
     4081 taccgcgatc gtcaaagatc gttattaccg taacgatcgg attgtcgaat ttgccaatat
     4141 gatccgcgat ctcgatcagg agtgtggttc aacctgcgcg gcggatttcc gcgatcgctt
     4201 aggcgtaggc cgaaagctgg caattcagat tctggaatat tttgaccgca ttggctttac
     4261 gcgtcgtcgt ggaaatgatc atttattgcg cgacgcatta ttatttccgg aa
//
LOCUS       DAZPEB010000243         4294 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZPEB010000243 DAZPEB010000000
VERSION     DAZPEB010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4294
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.248"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            46..2271
                     /gene="ybiO"
                     /locus_tag="ACHURJ_003843"
                     /pseudo
     CDS             46..2271
                     /gene="ybiO"
                     /locus_tag="ACHURJ_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(2268..3194)
                     /gene="rlmF"
                     /locus_tag="ACHURJ_003844"
     CDS             complement(2268..3194)
                     /gene="rlmF"
                     /locus_tag="ACHURJ_003844"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFR1282599.1"
                     /translation="MSDQKLGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            3470..3729
                     /gene="mcbA"
                     /locus_tag="ACHURJ_003845"
                     /pseudo
     CDS             3470..3729
                     /gene="mcbA"
                     /locus_tag="ACHURJ_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
     gene            3994..>4294
                     /locus_tag="ACHURJ_003846"
     CDS             3994..>4294
                     /locus_tag="ACHURJ_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFR1282600.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPG"
BASE COUNT          998 a         1129 c         1075 g         1092 t
ORIGIN      
        1 gcatctatac ttatcttttt gctctgtttt ctcactggag gagtcatgcg gtggatcctg
       61 ttcatcctct tctgcctgct gggcgcacct gcccacgcgg tatccatacc cggcgttaca
      121 accacaacga caacggactc aacgactgaa ccggccccgg aaccggatat cgaacaaaaa
      181 aaagcggcct atggcgcact ggcggatgtg ctggataacg acacctcgcg taaagaattg
      241 atcgaccagt tgcgtactgt cgccgcgacg ccccctgcgg aaccggtccc gaagatcgtg
      301 ccgccgacgc tggttgaaga gcaaaccgtg ctgcaaaagg tcaccgaagt cagccgccat
      361 tatggtgaag ccctttccgc ccgcttcggg caactttatc gcaatattac cggctccccg
      421 cataagccgt ttaatccaca aacattcagc aatgcgctga cccatttttc aatgttagcg
      481 gtattagtgt ttggttttta ctggctgatt cgcctgtgcg cactgccgct gtatcgcaaa
      541 atgggcctgt gggcgcggca aaaaaatcgt gagcgcagta actggttgca gcttccggcg
      601 atgattatcg gggcgtttat tatcgaccta ctgttactgg cactgacatt atttgtcggc
      661 caggtattaa gcgacaacct gaatgcaggc agtcgcacca tcgctttcca acaaagtttg
      721 tttctcaacg cctttgccct gattgaattt ttcaaagctg tactacgcct gattttttgc
      781 ccaaacgtgg ctgaactgcg cccgttcacg attcatgacg agaccgcccg ttactggagt
      841 cgtcgcctga gctggttaag cagtttgata ggttatggcc tgattgtggc cgtgccgatt
      901 atctctaatc aggtgaatgt acagataggt gcgctggcga acgtcatcat tatgctgtgc
      961 atgaccgtct gggcgttgta cctgatcttt cgcaataaaa aagagattac ccaacatttg
     1021 ctcaactttg cggagcattc gctggccttt ttcagcctgt ttatccgcgc ctttgcgctg
     1081 gtgtggcact ggctggcaag cgcctatttt atcgtgctgt ttttcttttc gttgttcgat
     1141 ccgggcaaca gcctgaaatt tatgatgggt gcaacggtgc gcagcctggc gattattggt
     1201 atcgcagcgt ttgtttccgg tatgttttcc cgctggctgg cgaaaaccat caccctctcg
     1261 ccacatactc agcgtaacta tccggagttg caaaaacggc tgaatggctg gctttcggcg
     1321 gcgctgaaaa cggcgcgtat tctgaccgtc tgcgtggcgg taatgttgct gttgagcgca
     1381 tggggattgt tcgatttctg gaactggctg caaaacggcg cggggcagaa aaccgtagat
     1441 atcctgatcc gtatcgcact tattcttttt ttctcggcgg ttggctggac ggtgctcgcc
     1501 agtttgatcg aaaaccggct ggcttcggat attcatggcc gcccgctacc cagcgcccgt
     1561 acgcgtaccc tgctgacgct gtttcgtaac gcgctggcgg tgattatcag taccatcacc
     1621 atcatgattg tgttgtcgga aatcggcgtc aatatcgcgc cattgctggc aggtgccggg
     1681 gcattaggtc tggctatctc gtttggttcg caaacgctgg tgaaagatat cattaccggg
     1741 gtgtttattc agtttgaaaa cggcatgaac actggagatt tggtgactat cgggccgttg
     1801 accggcacag tggaacggat gtcgattcgc tccgtgggcg tgcgccagga taccggggcg
     1861 tatcacatca ttccgtggtc ttcgataacc acctttgcta acttcgtccg cggtattggt
     1921 tcggtagtgg caaactacga tgttgatcgc catgaagatg ctgataaagc caatcaggca
     1981 ctgaaagatg cggtagcgga attgatggaa aacgaagaaa ttcgcgggct gattattggt
     2041 gaaccgaatt ttgccgggat tgtcggctta agcaataccg cgtttacact gcgtgtttcg
     2101 ttcaccacgc tgccactcaa acagtggacg gtacgctttg ccctcgacag ccaggtgaaa
     2161 aaacatttcg acctggtggg cgttcgcgcg ccagtgcaga cttattaggt gctgcctact
     2221 ccgggcgcga ccccggctga accgttaccg ccgggggagc caacgcttta acgctggcga
     2281 ttgacaaaac ggcggcgctg ctcgtcgttc ataaaggtcc aggcaataaa gcgactctgc
     2341 ttttgcccct gggccatctc tttttttacc accttcaccg cgcccacgtc ggtcagagca
     2401 cgatacaacg gcggtaagtt ttcaccacga gataccagcg aggtaaacca catcacctgc
     2461 ttcgcgaagc ctttgctctc ttcaatcatc tttttgataa aggcgacttc accaccttca
     2521 caccacaact cctgttgctg accaccaaag ttcagtgcat cgtctttgtt cagccccagg
     2581 ttacggcgtt tacgctcact acctgcccgc gctgcagcgg cggaatcgtg gaatggcggg
     2641 ttacacaagg tcgcgtcgta ttgctcgttt ttatggatga tgccgttaaa aattgcccca
     2701 ctctcttttt gccgacgcag acgaatggcg cggttaagcc ccggattagc actgataatc
     2761 gcctgcgcac tgcttaacgc ctggctgctg gtttcactac cggtaaaacg ccagccatat
     2821 tcatgtacgc caatcagcgg ataaatacag ttcgcaccaa cgccgatatc cagaatgctg
     2881 gcattcgctg gaattgttcc gcttgcttca gccagtaaat cggcaaggtg atgaatataa
     2941 tccgcccggc cgggtactgg tgggcagaga aaaccgtcgg ggatatccca gttcgctacg
     3001 gcgtaaaaat gggccagcaa cgccttattg agcgccttca ccgccagcgg attggcaaag
     3061 tctacgcttt gctccccggc gggtgtaagc gtgaggaatt gcctgagttc aggattgacc
     3121 tgacaaagcg tggcgagatc gtagcggctg tgatgacggt tgcgcggatg caaccccagt
     3181 ttctggtcgg acatagcatt ctcctttaag cagcggcgta agatacccgc ataacgcgac
     3241 agggtaaata tctggctgcg tttatcattc cccaaaacct taacatggct atggtttatg
     3301 gcaattcatc ataaaaatca gcattatgca tgtcattgat ttgtcttatt ttgtaaaaat
     3361 atatcgttca aaaaataagc acatctctgt caattttttg cgtcatttgc atcgcgcagg
     3421 cgacctacac ttatcaggca ctacccgttg tatttatgag gatgtcatta tgaaaaagtg
     3481 cctcacacta ctgattgcca ccgtcctgag cagaatctct ctcacggctt atgccgcgca
     3541 accgatgagt aacctggaca gcggtcagct acggcccgcg gtaccgtttc ggcaactggc
     3601 gcatcaaacc taagcgatct ggaggataaa ctggcagaaa aagcgcgcga acaaggcgcg
     3661 aaaggttatg tcattaattc cgcaggcgga aatgaccaga tgttcggtac tgcaaccatc
     3721 tacaaataac cccaccaacg taactgcatt cgcctctgga tgcagttttc gcctgttttg
     3781 taatcaaaaa acatcaaata tgaactcaat gtaaataaat gtatttcttt ttcgcgcaat
     3841 gggtgataga aaatcgcgcc aagtgataat gtttatcaaa attattatca cttgcatgaa
     3901 cactatcacg ggattaacag tggcatcgca tccgcagaga tgctttctcg tgacagtgaa
     3961 aatttcaaca tataagaaaa agtcacctgc aaaatggaaa acaatcgcaa tttccctgcc
     4021 agacaatttc attcgctcac gttctttgcc ggtctttgta ttggcatcac gcctgtggct
     4081 caggcactcg ccgccgaagg gcaaactaac gcggatgaca cgctggttgt cgaagcatca
     4141 acgccttcgc tttatgcgcc acaacaatct gccgatccga aattctcgcg tccggtagcg
     4201 gatactaccc gcacgatgac ggtgatttct gaacaagtga ttaaagatca gggcgcaacc
     4261 aaccttaccg acgcgctcaa aaacgtcccc ggcg
//
LOCUS       DAZPEB010000244         4267 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZPEB010000244 DAZPEB010000000
VERSION     DAZPEB010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4267
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.249"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..213)
                     /locus_tag="ACHURJ_003847"
     CDS             complement(<1..213)
                     /locus_tag="ACHURJ_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HFR1282601.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVD"
     gene            523..2295
                     /gene="aspS"
                     /locus_tag="ACHURJ_003848"
     CDS             523..2295
                     /gene="aspS"
                     /locus_tag="ACHURJ_003848"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFR1282602.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2413..2865
                     /gene="nudB"
                     /locus_tag="ACHURJ_003849"
     CDS             2413..2865
                     /gene="nudB"
                     /locus_tag="ACHURJ_003849"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFR1282603.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDSQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2894..3634
                     /locus_tag="ACHURJ_003850"
     CDS             2894..3634
                     /locus_tag="ACHURJ_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR1282604.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVITFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEIAATL"
     gene            3669..4190
                     /gene="ruvC"
                     /locus_tag="ACHURJ_003851"
     CDS             3669..4190
                     /gene="ruvC"
                     /locus_tag="ACHURJ_003851"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFR1282605.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(4192..>4267)
                     /locus_tag="ACHURJ_003852"
     CDS             complement(4192..>4267)
                     /locus_tag="ACHURJ_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282606.1"
                     /translation="HHPGLVLIHAEGVETPQPELTEAV"
BASE COUNT         1054 a         1060 c         1199 g          954 t
ORIGIN      
        1 atcaaccggc tgttttaatg cttcggcgta atcggcagac cagccaactc gcaccagaaa
       61 cacgggctga ccgctggcgc gaaatttcgc cgccagcttc ccggcgcgat taactacctc
      121 atcggcagta tgtggaccac cggcaaaagg taagatgcct tcttgtaaat cgatcaccac
      181 caacgcagtg gttttagcat taagttcaag catgctgact cctgaaaaaa caaaatcgtg
      241 cctcacacct taatgcgatt agccgatggc ggaacgacaa attttgttaa tttttatgag
      301 ttcgggcaac acctttatag caaactggcg tagagtctga tttcaggccc gatataaagt
      361 ggtaacgagg cgttccagcg cgggctaggc tcttacggtg cggcagaatt tccagtataa
      421 tagccgcctt ttttcattca gttgtgacat acagctaacg ctgcgacttg gtcacctgcg
      481 cagcaggggg catccgccgg cagctaagtt aagggatatc tcatgcgtac agaatattgt
      541 ggacagctcc gtttgtccca cgtggggcag caggtgactc tgtgtggttg ggtcaaccgt
      601 cgtcgtgatc ttggtagcct gatcttcatc gatatgcgcg accgcgaagg tatcgtgcag
      661 gtatttttcg atccggatcg tgcggacgcg ttaaagctgg cctctgaact gcgtaatgag
      721 ttctgcattc aggtcacggg caccgtacgt gcgcgtgacg aaaaaaatat taaccgcgat
      781 atggcgaccg gcgaaatcga agtgctggcg tcctcgctga ctatcatcaa ccgcgcagat
      841 gttctgccgc ttgactctaa ccacgtcaac accgaagaag cgcgtctgaa ataccgctac
      901 ctcgacctgc gtcgtccgga aatggctcag cgcctgaaaa cccgcgctaa aatcaccagc
      961 ctggtgcgcc gttttatgga tgaccacggc ttcctcgaca tcgaaactcc gatgctgacc
     1021 aaagccacgc cggaaggcgc gcgtgactac ctggtgcctt ctcgtgtgca caaaggtaaa
     1081 ttctacgcac tgccgcaatc cccacagttg ttcaaacagc tgctgatgat gtccggtttt
     1141 gaccgttact atcagatcgt taaatgcttc cgtgacgaag acctgcgcgc tgatcgtcag
     1201 cctgagttta ctcagatcga tgtggaaact tctttcatga ccgcgccgca agtgcgtgaa
     1261 gtgatggaag cgctggtgcg tcatttgtgg ctggaagtga agggcgtgga tctgggcgat
     1321 ttcccggtaa tgacctttgc ggaagcagaa cgccgttatg gttctgataa accggatctg
     1381 cgtaacccga tggaactgac cgacgttgcc gatctgctga aatctgttga gtttgcagta
     1441 tttgcggggc cggcgaacga tccgaaaggc cgtgttgccg ctctacgtgt tccgggtggc
     1501 gcatcgctga cccgtaagca gatcgacgaa tacggtaact tcgttaaaat ctacggcgcg
     1561 aaaggtctgg cttacatcaa agttaacgaa cgcgcgaaag gtctggaagg tatcaacagc
     1621 ccggtagcga agttccttaa tgcagaaatc atcgaagcca tcctggatcg tactgccgcg
     1681 caagatggcg atatgatttt cttcggtgcc gacaacaaga aaattgttgc cgacgcgatg
     1741 ggcgcactgc gcctgaaagt gggtaaagac cttggtctga ccgacgaaag caaatgggca
     1801 ccgctgtggg ttatcgactt cccgatgttt gaagacgacg gtgaaggcgg cctgacggca
     1861 atgcaccatc cgttcacctc accgaaagac atgactgctg cagaactgaa agctgcaccg
     1921 gaaaatgcgg tggcgaacgc ttacgatatg gtcatcaatg gttacgaagt gggcggtggt
     1981 tcagtacgta tccataatgg tgatatgcag cagacggtgt ttggtattct gggtatcaac
     2041 gaagaggaac agcgcgagaa attcggcttc ctgctcgacg ccctgaaata cggtactccg
     2101 ccgcacgcag gtctggcatt cggtcttgac cgtctgacca tgctgctgac tggcaccgac
     2161 aatatccgtg acgttatcgc cttcccgaaa accacggcgg cagcgtgtct gatgactgaa
     2221 gcaccgagct ttgctaaccc gactgcactg gctgagctga gcattcaggt tgtgaagaag
     2281 gcagagaata actgatatga ctcaaataca cgaaatcatt cgcgttgcat caaggcggca
     2341 attgagtgaa ctcccatgag catagataac tatgtgaatg ggatgagcga aggcagtcaa
     2401 cgaagaggca gcgtgaagga taaagtgtat aagcgtcccg tttcgatctt agtggtcatc
     2461 tatgcacaag atacgaaacg ggtgctgatg ttgcagcggc gtgacgatcc cgatttctgg
     2521 cagtcggtaa ccggcagcgt ggaagagggt gaaaccgcgc cgcaagctgc catgcgcgaa
     2581 gtaaaggaag aggtcaccat tgatgttgtc gctgaacaac tgaccttaat tgacagtcag
     2641 cgcacggtag agtttgaaat tttttcacat ttacgtcatc gctatgcgcc gggcgtgacg
     2701 cgtaatacgg aatcatggtt ctgtcttgcg cttccgcacg agcggcagat cgttttcact
     2761 gaacatctgg cttacaagtg gcttgatgcg cctgctgcgg cggcgctcac taagtcctgg
     2821 agcaaccggc aggcgattga acagtttgta attaacgctg cctgaacagg cagggctatc
     2881 tggagatatt tttatggcag gtcatagtaa atgggccaac accagacatc gtaaagctgc
     2941 gcaggatgct aagcgcggta aaatcttcac taaaatcatt cgtgagctgg taaccgcggc
     3001 taagctgggc ggtggcgatc cggacgctaa cccgcgtctg cgtgcggcgg tggataaagc
     3061 actgtctaac aacatgaccc gtgacacact gaaccgcgca attgcacgtg gtgtgggcgg
     3121 tgatgatgat gcaaacatgg aaaccatcat ctacgaaggt tacggtcctg gcggcacggc
     3181 aatcatgatt gaatgtctgt ctgacaaccg caaccgtacc gttgctgaag tgcgtcatgc
     3241 attcagcaaa tgtggcggta acctcggtac agatggttcc gtagcctatc tgttcagcaa
     3301 aaaaggcgtg atcaccttcg agaaaggcga tgaagacacc atcatggaag cagctctgga
     3361 agcaggtgct gaagacgttg tgacctatga tgacggcgcg attgatgtct acactgcatg
     3421 ggaagaaatg ggtaaagtgc gcgacgctct ggaagcggca ggtctgaaag cagacagcgc
     3481 ggaagtttcc atgatcccgt ccaccaaagc tgatatggat gcagaaaccg caccgaaact
     3541 gatgcgtctg atcgatatgc tggaagattg cgacgacgtg caggaagttt accataacgg
     3601 tgaaatctct gatgagattg cagcgactct ctgatgaggc ctgctaaaca gcaaaacgga
     3661 gacgcgtgat ggctattatt ctcggcattg acccgggttc gcgcgtgacc ggctatggcg
     3721 tcatccgcca ggtaggcagg caactgtcct acctgggtag cggatgcata cgcaccaagg
     3781 tggatgattt accgtctcgt ctgaagctca tctatgcggg cgtgacggaa atcatcaccc
     3841 agttccagcc agattatttc gccattgaac aagtctttat ggccaaaaat gccgactcgg
     3901 ctctgaaact ggggcaggcg cgcggcgtgg cgattgtggc ggcggtgaat caggaattgc
     3961 cggtatttga atatgcggca cgtcaggtaa agcaaacggt ggtaggtatt ggtagtgccg
     4021 aaaaaagtca ggtgcagcat atggtccgca ccttgctgaa actgcccgct aatccacagg
     4081 cggatgccgc cgatgcgtta gctatcgcga ttacccattg tcacgtcagc cagaatgcaa
     4141 tgcagatgag cgaatcgcgg ctgaacctgg cgagagggcg actgcgttaa gttataccgc
     4201 ctcggtcagt tcaggctgag gcgtttcaac tccctccgcg tgaatcaaca ccaaacccgg
     4261 atgatgc
//
LOCUS       DAZPEB010000245         4203 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZPEB010000245 DAZPEB010000000
VERSION     DAZPEB010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.250"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..69
                     /locus_tag="ACHURJ_003853"
     CDS             <1..69
                     /locus_tag="ACHURJ_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFR1282607.1"
                     /translation="HDAYKASDERLYVNKQNKNSRS"
     gene            112..615
                     /gene="yeaK"
                     /locus_tag="ACHURJ_003854"
     CDS             112..615
                     /gene="yeaK"
                     /locus_tag="ACHURJ_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFR1282608.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     VNFRRTA"
     gene            complement(616..720)
                     /gene="yoaI"
                     /locus_tag="ACHURJ_003855"
     CDS             complement(616..720)
                     /gene="yoaI"
                     /locus_tag="ACHURJ_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFR1282609.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            889..1335
                     /locus_tag="ACHURJ_003856"
     CDS             889..1335
                     /locus_tag="ACHURJ_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFR1282610.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(1292..2113)
                     /gene="nimR"
                     /locus_tag="ACHURJ_003857"
     CDS             complement(1292..2113)
                     /gene="nimR"
                     /locus_tag="ACHURJ_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFR1282611.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            2210..3406
                     /gene="nimT"
                     /locus_tag="ACHURJ_003858"
     CDS             2210..3406
                     /gene="nimT"
                     /locus_tag="ACHURJ_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005117270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFR1282612.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGSNSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGNWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     RSYPVL"
     gene            3446..3793
                     /locus_tag="ACHURJ_003859"
     CDS             3446..3793
                     /locus_tag="ACHURJ_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFR1282613.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFTTFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(3815..4069)
                     /gene="yoaF"
                     /locus_tag="ACHURJ_003860"
     CDS             complement(3815..4069)
                     /gene="yoaF"
                     /locus_tag="ACHURJ_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFR1282614.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVSTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
BASE COUNT          967 a         1064 c         1043 g         1129 t
ORIGIN      
        1 catgatgcgt ataaagcttc cgatgagcgt ttatatgtca ataagcagaa caaaaacagc
       61 cgttcatgat aaccttctgt ggttgtttgc ttgtaatctc aggagcgtga aatgactgaa
      121 atggctaaag gaagcgtgac gcatcagcgg ttaatcgctt tattatcaca agaaggtgct
      181 aacttccgcg tcgttaccca tgaagcggta ggtaaatgtg aagcggtgtc agaaattcgt
      241 ggcaccgctt taggccaggg agctaaggca ctggtatgta aagtcaaagg caatggcgta
      301 aatcaacatg ttctggcgat cctcgctgcc gatcaacagg ccgatctgag ccaacttgcc
      361 agccatatag gcggattacg cgcttcgtta gccagccctg ctgaagtcga tgaactcaca
      421 ggctgtgtct tcggcgcgat cccccctttc agcttccatc caaaactcaa actggttgcc
      481 gaccctctac tcttcgaacg atttgatgaa atcgccttca atgcagggat gctggataaa
      541 tccgttattt tgaaaaccac ggattatttg cgcattgcgc aaccagaact ggtcaatttc
      601 cgccgcactg cgtaactagc ctgtccgttc aataagcaga acggacaaaa ccagcacaac
      661 agcaatggca aaaaacgatg acgtgataat cagtgtctcg acgaacattt gatcgttcat
      721 agcatgcccc cgggagatag ctgttttcac gttattgtta gcgtgcacaa atggcagttt
      781 gatgacagtt cgcctgattt ttatgcaaaa aacgtaaaga ttttctactt ccttcctgca
      841 gcaagcgtaa agtaagcagg cttattattt tttggcaagg aaaccacgat gtttgatgtc
      901 actttgctga tcctgctcgg gttagctgcg ctgggcttta tcagtcataa caccactgtc
      961 gccgtttcaa ttctggtgtt gatcattgtc cgcgtcacac cgttaagcac ctttttcccg
     1021 tggattgaaa aacaggggct aagtatcggg ataatcattc tgacgattgg cgttatggcc
     1081 cccatcgcca gcgggacgct accgccctca accttgattc actcgtttct gaactggaaa
     1141 tcgctggtgg caattgcagt aggcgtgatt gtctcctggc tgggtgggcg cggcgtgacc
     1201 ttaatgggca gccagccgca actggtcgcc gggctgctgg taggcactgt tttaggcgtg
     1261 gcgctgtttc gcggcgtacc ggtcggcccg cttattgccg cgggtctggt ttcgctgatt
     1321 gtggggaaac agtagttaat cccgcaatat atcggcctgg cgtttgcccc aggcctttct
     1381 taaacatagt aataaaggcc gttgttgaat cataccccag cgtatgagca actttctgta
     1441 cagtatcgcc tttcactaat ccctgaagtg ccataatcag ctgtaactgc tgacgccact
     1501 ggcgaaaact caatcccgtc tcttttacga tcagtcgcgc aagattgcgt tcactcatcg
     1561 caaaaaagcc agcccattgc cctaatgacc cccactcgac gggccctttc gccatcatct
     1621 ccaccatcgt gcggatttta ggatgagaag aaacgggcag gtgcagctgt tgttgtggct
     1681 gctgagggag ttcatcaaac aggacctgta ttaatctgcg ggtcatcggc tcagcacgct
     1741 gagtcgtcgt cctgtttgcc agggttaaaa tcaattcccg gcataagggc gatattttca
     1801 atgtgcaaca tgttgtcggc attgtcactg cgctgggttc gataaacaga aaacagagtt
     1861 cagcgttagc tgtcacttga ttgctatgtt ccaccccgcc cggtatccaa accgcatatt
     1921 gcggcggcac catccacaaa gcattttcca ccgtacaggt aattgcacca tgtaatgcta
     1981 gtatcagctg tcctttgcgg tgctgatgta caggactggt taactcatcc gttccggcgt
     2041 gaatacaaaa tgcgaccgcg gcttcatggt gtcggtcggg ttcatagcca ttgagattca
     2101 acctgtgcat cattttgtcc gaatttagcg ataatttgtc attttagctt gattcaacat
     2161 aacaataaaa acggtaaggt acagcctcgt ttgtaacaat gagaagcata tgacctgttc
     2221 aacttcatta agcggcaaaa acaggattgt ccttatcgct ggcattctga tgattgccac
     2281 aacattacgc gtcaccttta ccggcgcagc accgttactg gatacgattc gttccgctta
     2341 ctcgctgacg acagcgcaaa ccggcttatt gaccaccctg ccattattgg cctttgcgct
     2401 aatctcacct ttggccgccc cggtagcgcg acgttttggt atggaacgta gcctgtttgc
     2461 cgcgttactt ttgatctgtg ctggtatcgc aattcgctct ctcccttcgc cttacttatt
     2521 atttggcggt acagcggtca ttggcggtgg gattgcatta ggcaatgtct tactgccagg
     2581 attaattaaa cgcgatttcc ctcattccgt cgccagactt accggcgcat attccctgac
     2641 aatgggagct gcggcggcac ttggatcggc tatggtagtg ccgctggctt tgaacggttt
     2701 tggctggcaa ggcgcgttgc tcatgctgat gtgttttcct ctgctggctc tttttttatg
     2761 gctgccacag tggcgaagcc aacaacatgc aaatttgagt acctcgcgcg ccttacatac
     2821 tcggggtatc tggcgttcgc cgcttgcctg gcaggtcaca ttgtttcttg ggagcaactc
     2881 actggtctat tacgtgatta ttggctggct tccggcgatc ctcatcagtc acggctatag
     2941 cgaagcacag gcgggttcac tgcatggttt gctgcaacta gccacagcag ctcccggttt
     3001 gctgatccct cttttcttac atcatgtgaa agaccagcgt ggtattgcag cgttcgttgc
     3061 cttgatgtgc gcagtgggcg ctgttgggct ctgctttatg ccagcgcacg cgatcacctg
     3121 gactctgctt ttcggttttg gttccggcgc aacaatgata ctggggttga cgttcattgg
     3181 tcttcgggca agttctgcgc atcaggcggc ggcactctcg gggatggcac aatccgtcgg
     3241 gtatttgctg gcagcctgtg ggccgccgct aatgggtaaa atacacgatg ctaacggtaa
     3301 ctggtccgta ccactgatgg gtgttgcaat actttcacta ctgatggcga ttttcggact
     3361 ttgcgccggg agagacaaag aaattcgctc atatccggtg ctatagtgac gtgacaaatc
     3421 atgcgtgaaa gggagaacaa acacgatgaa tattcagtgc aaacgcgttt atgatccggc
     3481 tgaacagagc gatggttatc gcgtactggt cgaccgcctc tggccgcgcg gtatcaaaaa
     3541 aaccgattta gcccttgatg agtgggataa agaaatcacg ccgtcaacgg aactgcgcaa
     3601 agcctttcac ggcgaagtcg tcgattttac aacctttcgc gagcaatatc ttgcagaact
     3661 ggcgcaacac gagcaagaag gaaagcggct ggcggacatc gccaaaaaac agccgctgac
     3721 cctgctctac gcagcaaaaa acaccacgca gaaccatgcg ctggtgctgg ccgactggct
     3781 acgtagcttg tgattttagt acagcatccg gcggttattt ttcaccagcc ggatggtccc
     3841 gtcgccacaa tgcccattca tcaatcgttt caccgcccgg taatttgcaa ttgttgctga
     3901 ccccttgcgc tgtcgacact ggaatgagcg tcccgccctt ctgctggcaa tagaccgacg
     3961 ccggatttgc cataccaatc tgcggcggtt taggtgcttc tggctgagaa ggggttgaac
     4021 aaccagccag gaccagcaag caaggcagaa caaaactgat aattttcatt tattgatctc
     4081 acatatttat ccagacttag agtatcgcgg tatcgttttg ttttgcagca ctatttttat
     4141 tacattcact caaaacatat tacgtcttgt ttcatctttg ttgatgatgt tttatcatgc
     4201 ctg
//
LOCUS       DAZPEB010000246         4183 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZPEB010000246 DAZPEB010000000
VERSION     DAZPEB010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.251"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..209)
                     /locus_tag="ACHURJ_003861"
     CDS             complement(<1..209)
                     /locus_tag="ACHURJ_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR1282615.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="ACHURJ_003862"
     tRNA            complement(324..399)
                     /locus_tag="ACHURJ_003862"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="ACHURJ_003863"
     tRNA            complement(406..480)
                     /locus_tag="ACHURJ_003863"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="ACHURJ_003864"
     tRNA            complement(597..681)
                     /locus_tag="ACHURJ_003864"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="ACHURJ_003865"
     tRNA            complement(690..765)
                     /locus_tag="ACHURJ_003865"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="ACHURJ_003866"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="ACHURJ_003866"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFR1282616.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAAQRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACHURJ_003867"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACHURJ_003867"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFR1282617.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACHURJ_003868"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACHURJ_003868"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFR1282618.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQYATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLLKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1078 a         1041 c          933 g         1131 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctggaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagcgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc gacacgatga agaaacagcc gaatggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttcttcttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctaggagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctcagcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctctg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg tagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaagtc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcggaatg gattatccac acgatccaca tcatgtattt gtttctgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgtttcct
     1861 gaaattccgc gaaggggctt ttaccgaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgcg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcgagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agcttgcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggtactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttagcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catactgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagtt atgacgacag ggagagtttg tag
//
LOCUS       DAZPEB010000247         4126 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZPEB010000247 DAZPEB010000000
VERSION     DAZPEB010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4126
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.252"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(36..593)
                     /gene="ymfL"
                     /locus_tag="ACHURJ_003869"
     CDS             complement(36..593)
                     /gene="ymfL"
                     /locus_tag="ACHURJ_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HFR1282619.1"
                     /translation="MGKHHWKIEKQPEWYVKAVRKTIAKLPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMVLQRAGGTHFIADAVAQSANGVFVSLPDIEDVDNADINQR
                     LLEVIEQIGSYSKQIRSAIEDGVVEPHEKTAINDELYLSISKLQEHAALVYKIFCISE
                     SNDARECAAPGVVASIASGCGETNA"
     gene            complement(637..837)
                     /locus_tag="ACHURJ_003870"
     CDS             complement(637..837)
                     /locus_tag="ACHURJ_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaS family helix-turn-helix protein"
                     /protein_id="HFR1282620.1"
                     /translation="MKAYWDSLTKEQQGELAGKVGSTQGYLRLVFNGYKKASFVLAKK
                     LEQCTSGAITKSDLRPDIYPKD"
     gene            928..1602
                     /locus_tag="ACHURJ_003871"
     CDS             928..1602
                     /locus_tag="ACHURJ_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFR1282621.1"
                     /translation="MKTIHDIRRSNARKLRDGVGGNSSFATMIDREPTQTSRFMGDGA
                     TKNIGDSMARHIEKCFDLPIGWLDQEHQTTNITKKPEVSITNKQITLVPVISWVQAGA
                     WKEVGYSEVDLSTAETYPCPVPCGEMTYILRVIGDSMIDEYRPGDMIFVDPEVPACHG
                     DDVIALMHDTGETTFKRLIEDGTQRYLKALHPNWPEPYIKINGNCSIIGTVIFSGKPR
                     RYKIKA"
     gene            3131..3667
                     /locus_tag="ACHURJ_003872"
     CDS             3131..3667
                     /locus_tag="ACHURJ_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD family hydrolase"
                     /protein_id="HFR1282622.1"
                     /translation="MSFIQTLTGKKFNYINSTIDDIYIEDIAGALSNICRFAGHLPDF
                     YSVAQHSVLCSQLVAPEFAFEALMHDATEAYCQDIPAQLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPLLEGIPATEMFKVIPLSPGHA
                     YGMFMERFNELSELRKCA"
     gene            3658..4020
                     /locus_tag="ACHURJ_003873"
     CDS             3658..4020
                     /locus_tag="ACHURJ_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282623.1"
                     /translation="MRMNVFEMEGFLRGKCVPRDLKVNETDAEYLVRKFDALEAKCAA
                     LENKVIPVSTELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGDWQWTFQVGDLE
                     NVNITHWAVMPKAPEAGA"
     gene            4020..>4126
                     /locus_tag="ACHURJ_003874"
     CDS             4020..>4126
                     /locus_tag="ACHURJ_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282624.1"
                     /translation="MTTFTDKELIKEIKERISSLDVRDDIERRAYEIALL"
BASE COUNT         1256 a          899 c          876 g         1095 t
ORIGIN      
        1 cagttgcgag cgacggtagt gtgttgttaa actgttcatg cgttagtttc tccacaacca
       61 gaagcaatcg acgccacgac gcccggagct gcacactcgc gggcgtcatt actttctgaa
      121 atgcaaaaaa ttttgtagac aagtgctgca tgctcctgca gcttcgaaat tgagagatac
      181 agctcgtcgt taattgctgt cttctcatgc ggttccacta caccgtcttc gattgctgaa
      241 cgaatctgtt ttgaataact gccgatctgt tcaatgactt ccagcagacg ctggttaata
      301 tcggcgttgt ccacatcctc gatgtcagga agagacacaa agacgccatt tgcagactgc
      361 gccacagcat cagcaatgaa gtgagtgcca ccagcacgct gtaaaaccat tgcccatccc
      421 agcgggaaaa tctgatcgcc atctgcacga aggcggttga ataaagcgtt ttctgttaca
      481 tcgagccagt cagccgcttc agcgtaacca cccggcaact ttgcgatagt ttttctgaca
      541 gctttcacgt accactcagg ctgtttttct attttccagt gatgcttacc cacgattagc
      601 ctcatcgttc tgtggttaaa aattgaaagt gttctgctaa tctttcggat agatatccgg
      661 tcttaagtca gatttcgtaa ttgcacctga cgtgcattgc tcaagttttt tagccagcac
      721 aaaactggct tttttatagc cattgaaaac cagccgtaag tagccttgtg ttgagccaac
      781 ttttccggcc aactcaccct gctgttcttt ggttaaagag tcccaatacg ctttcataca
      841 atatgtacct ccagtgtaca catcacatga ttgaaatgaa ccttcaagat acttgcacct
      901 tagcggtaca agggttttaa tttcgttatg aaaacaatcc atgacatccg gcggtctaac
      961 gccagaaaac tgagagatgg tgttggcggg aattcttcct ttgccactat gattgatcgc
     1021 gagccaaccc agaccagcag gtttatggga gatggtgcta ctaaaaatat cggtgacagc
     1081 atggcacgac acattgaaaa atgtttcgac ctgcctatcg gatggctcga tcaagaacac
     1141 cagacaacga acatcacaaa aaaacctgaa gtttcaatca ctaacaaaca aataacgtta
     1201 gtccctgtca tatcatgggt acaggccgga gcatggaaag aagttggcta ttctgaggtt
     1261 gatttgagca cagcagaaac ttatccctgc cctgtaccct gtggcgaaat gacttatatc
     1321 ttgcgggtga ttggtgattc aatgattgat gagtaccgtc cgggagacat gatttttgtt
     1381 gatcctgaag tccctgcttg ccacggtgac gacgttattg cgttgatgca cgatacaggc
     1441 gaaaccacct tcaagcgatt gatagaagat ggaacacagc gttatctcaa agcattacac
     1501 ccaaactggc ctgagcccta cattaagatt aacggtaatt gctctataat tggcactgtg
     1561 attttctcgg gaaaaccaag aagatacaaa ataaaggcct aatcaatatt tatgaacctg
     1621 cttcggcagg tttttttata cttgacaatg taccccatgg acacacaatg taccccatgg
     1681 acacacaatg taccaaaacg aaacaacaaa caggcaggat gcccacgaag tagccgcctg
     1741 gggcatatga agtccaagat gattcgttag caacaaaacg cccataggac gcttcgctct
     1801 ttaacaattt gaatattttc agcgtcaatg atctgtttag ctccctacaa gtaaatgtag
     1861 cgtagcacca tgataaatcc tgctagtgtt tagttaaaaa tcgcgcgaga gcaagaacga
     1921 cgtgagggca ggcaatactg atagtgttat tgctattagt ttatcaaaaa tatttagacg
     1981 acccagatga atatttttaa tacatactcc ccctaattta acaggagtaa atgttgatga
     2041 ataacgagac atctatactc aacaattgta tgatattaac aattatataa cataatgtag
     2101 gaaaaataaa aatggattgg catgacctta tatcaaaggt aattatcgga cttgttacag
     2161 gctcaatcgc agcttactta actgcgcgat atgcactcgg ccgttattat aaagaaaaat
     2221 ggtgggacaa aagaatagat ttgtatacgt aattagttga ctgtgtttat cttctaaaaa
     2281 acacagctga ctattggtat aaacatgagt tacatcacgc ttcacaattc ccaacctact
     2341 ttagttacaa atcaaaagaa gaaaatgaaa tccttagcaa atctttctct aatgagcttg
     2401 aaaaactcag tagaataggt gagctatctt cattattctt aacagaagag tgtggggata
     2461 aaatccgttc attcattgaa aatgaagaaa atctatttat acaatgggtg tttgatagta
     2521 tagagttgat agatgcccac aagtcaagac ttgataaatt aaaagtatta ctagacgata
     2581 ttatctctga agcaaagaga gagcttaagg tcaataaaaa acataacagc ctaaagaata
     2641 taaagtggtc cataactatg ccagaccaaa aaaatgatga gccaattaat tatcacgaca
     2701 atcattaaaa cactttagaa cccgctgggc gggttctatt caaatatact ttgcacatca
     2761 caaataaaac tataacgtcc ttcttctttt cctaatatct tatatctact tatttataag
     2821 cggcttacag atcttcaaca gataaaccta atgaaggcga caatgttctg atgaatagcc
     2881 acctttttac tcaactgaaa gcgcgttctg tcccttgtca ttaagtgcca gttcgttaag
     2941 tccaaaacct ccggaacgcg ctttcaattg agtggggaaa ataactggcg atggcagccg
     3001 cccgttgcac taagtgcctc tggatggggc atttactgaa acgaaaccaa aacttttaat
     3061 cgccttatgg cgagggattc gtgcaaccaa aaatcgtgcg gtgcagcgca ccaattaagg
     3121 agaactaacg atgagtttta tccagactct taccggtaag aagttcaatt atatcaactc
     3181 caccattgac gatatttata tcgaggatat cgccggtgcg ctttcaaata tctgccgctt
     3241 cgcgggacac ttgccggatt tttacagcgt ggcgcagcat tccgttctgt gcagtcaact
     3301 tgtggcgcca gagttcgcct ttgaagcgtt aatgcatgat gcaacagaag cgtattgtca
     3361 ggacatcccc gcgcaactga aacgccttct tcctgactat aaacggatgg aagaaaaaat
     3421 agacgccgta atccgtgaga aatacgggtt acccccggtt atgagcacgc ccgtgaaata
     3481 tgccgatctc atcatgctgg caaccgaacg ccgcgatctt gggcttgatg atggctcttt
     3541 ctggcctcta ctagaaggta tcccagcaac agagatgttc aaagttattc cactgtcgcc
     3601 aggccatgcc tacgggatgt ttatggaacg ttttaacgaa ttatcggagt tacgcaaatg
     3661 cgcatgaatg ttttcgaaat ggaagggttt cttcgcggga aatgtgtacc gcgagatctg
     3721 aaagtgaatg aaacggatgc tgaatacctg gtgcgtaaat tcgatgcgct tgaagctaaa
     3781 tgtgcggcac tggaaaacaa agtaatacca gtgtcaactg aactgccacc agcaaatgaa
     3841 agtgttttgt tattcgatgc taacggagaa ggctggctaa ttggctggcg ttctctctgg
     3901 tacacctggg gacaaaaaga aaccggagac tggcagtgga catttcaggt cggggacctt
     3961 gaaaacgtca atatcactca ctgggcagta atgccaaaag caccggaggc tggagcataa
     4021 tgaccacttt taccgacaaa gaactgatta aagaaattaa agagcgtatc agcagccttg
     4081 acgtgcgaga cgatattgag cgccgtgctt atgaaatcgc actcct
//
LOCUS       DAZPEB010000248         4112 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZPEB010000248 DAZPEB010000000
VERSION     DAZPEB010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4112
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.253"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..165
                     /locus_tag="ACHURJ_003875"
     CDS             <1..165
                     /locus_tag="ACHURJ_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HFR1282625.1"
                     /translation="NEWESAFVELQKMFSLTQRDNAMLNEQVMQLSQQVQHLSERTER
                     LSQLYSENRR"
     gene            complement(713..1105)
                     /locus_tag="ACHURJ_003876"
     CDS             complement(713..1105)
                     /locus_tag="ACHURJ_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282626.1"
                     /translation="MALSACFRQQLNPVVNSLLKERIIHSPKELEHDNAVIRALQKLQ
                     IQEVCNSTLWETAKQQLLQKSDGRYFAINSKHLAFPGPGESHAFCCIKYKNAIGINGN
                     NAETQSTHYQPYPYDKVSIWGPFPHNLT"
     gene            complement(1422..1580)
                     /locus_tag="ACHURJ_003877"
     CDS             complement(1422..1580)
                     /locus_tag="ACHURJ_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR1282627.1"
                     /translation="MHEPPVQPDRCASSGNYRLESNPVRHDLTPLAAAIGNCTRWIEI
                     LRVLEVVA"
     gene            1910..2100
                     /locus_tag="ACHURJ_003878"
                     /pseudo
     CDS             1910..2100
                     /locus_tag="ACHURJ_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001441803.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2249..2440)
                     /locus_tag="ACHURJ_003879"
     CDS             complement(2249..2440)
                     /locus_tag="ACHURJ_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFR1282628.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGETR"
     gene            2505..2828
                     /locus_tag="ACHURJ_003880"
     CDS             2505..2828
                     /locus_tag="ACHURJ_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFR1282629.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVFLGEPVARSGRLPT
                     LAPPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            3037..3468
                     /locus_tag="ACHURJ_003881"
                     /pseudo
     CDS             3037..3468
                     /locus_tag="ACHURJ_003881"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3506..4036
                     /locus_tag="ACHURJ_003882"
     CDS             3506..4036
                     /locus_tag="ACHURJ_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HFR1282630.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTLWYLGGMIQANLEEIRKQNESLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            4043..>4112
                     /locus_tag="ACHURJ_003883"
     CDS             4043..>4112
                     /locus_tag="ACHURJ_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD/MobD family mobilization/exclusion protein"
                     /protein_id="HFR1282631.1"
                     /translation="MTELERHLLNALEQLQQDYMQRL"
BASE COUNT          984 a          934 c         1226 g          968 t
ORIGIN      
        1 aacgaatggg agagcgcctt cgtggaattg cagaaaatgt tttcgcttac gcaacgggac
       61 aacgcgatgc tgaacgagca ggtcatgcag ttgagtcagc aggtgcagca cttgagcgag
      121 cggacagaac gcttgagcca gttatacagc gagaacagga gataagggac gaacggctga
      181 tacaggaatg cgaacatgag ttatccctgg agcgtgagcg ccagccggaa atacaggaac
      241 gcacactgga tggtccttca ctgggatggt aatcagagac aggctaaaat gttgtcagcg
      301 cgtttccggt gcattttttg ggataatggt tggttcgggg gggcaccggg aacgcgcttc
      361 agtcagacct tgctgaaaac acaggaacgc gttaaaacgc atacgggcgc gattctggtg
      421 tgtcttttgt tgctgtgttt tgcctctatt gccagatcgc gctcattccc ctgaacttct
      481 gttttcccgc tcaggtcccc gtcttcttcg cttccgaaag catgaaaaaa atccgtttca
      541 gctgtctctg gctggcaatt cctgcgtgat tcacatggct gcatagctat gcatgtggtt
      601 aaaatttacc gggtgcgatc gcggcagttt ttcgggtggt ttgttgccat ttttacctgt
      661 ctgctgccgt gatcgcgccg aacgcgtttc agcggtgcgt acaattaaga tattacgtta
      721 aattgtgagg gaatggtccc catatactga ctttatcata gggatagggt tggtagtggg
      781 tcgattgggt ttcggcattg tttccgttaa tgccaattgc atttttgtat ttgatgcaac
      841 agaaggcgtg agattctcct gggcctggaa aggctaagtg tttagaattg atggcaaaat
      901 atcttccgtc cgatttttga agtagttgtt gtttggctgt ctcccaaagg gtgctgttac
      961 aaacttcttg aatttgtagt ttttgcaggg ctcttattac tgcgttgtca tgttccaatt
     1021 ctttagggga gtgtattatt ctctctttta ataagctgtt taccacaggg tttagttgtt
     1081 gtctaaagca cgcagataat gccattgcta cacaattttg ttcccgagga agcatgtttg
     1141 gcggggtatg ccgtgtttga tgtgatatgt ctattctcat agaaggtctc tggcttatag
     1201 catcaacaaa tacggctata aaacaaaatt ttcatagagt gattcaagat cttcttgaga
     1261 tccttttttg cggcgcgtaa tctgctgcct gcaaacaaaa aaaccaccgc taccaacggt
     1321 ggtttgtttg ccggaatcta gagctaccaa ctctttcttc cgaaggtaac tggcttggtg
     1381 gagcacagat accaaatgct gtccttctag tgtagccgca gttaggccac cacttcaaga
     1441 actcttaata tctcaatcca ccttgtacag ttaccaatgg ctgctgccag tggcgttaag
     1501 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg atgaggcgca gcggtcgggc
     1561 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     1621 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     1681 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     1741 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gactagagcg tcgatttttg
     1801 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca tcggcgcggc cttcttcctg
     1861 ttcctggtct tttgctcaca tgttctttcc ggcgttatcc cctgattctg tggataacag
     1921 catttcgttt ttgagtgagt agataccgct cgccgcagtc gaacgaccga gcgtagcgag
     1981 tcagtgagcg aggaagcgga aaagcgcctg gacgtgcatt ttctccttac gcatctgtgc
     2041 ggcatttcac acccggtatg gcgcactttt catacaatcc gcactgatgc cgcatggtga
     2101 agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc
     2161 taaaacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct cacagataag
     2221 ctgtgaccgt ctccgggagc tgcatgcgtc agcgggtttc accgtcatcc ccgaagcgag
     2281 cgaggcaact gcggtaaagc tcatcggcgt ggtcgtgaag cgattcacag atgtctgcct
     2341 gttcatccgc gtccagttcg ttgagcttct ccagcagcgt taatgtctgg cttctgataa
     2401 agcgggccat gttaagggcg gttttttcct gtttagtcac tgatgcctcc gtgtaagggg
     2461 gatttctgtt catgggggta atgataccga tgaaacgcga gaggatgctc acgatacggg
     2521 ttactgatga tgaacatgct cgtttgctgg aacgttgtga gggtaaacaa ctggcggtat
     2581 ggatgcggcg ggtcttcctg ggggagccgg ttgcccgttc cgggaggctg ccgacactgg
     2641 caccgccgtt actgcgtcag ctggccgcca tcgggaataa cctgaatcag acagcgagga
     2701 aggtgaacag cgggcagtgg tcttccggtg accgggttca ggtggtggcc gcactgatgg
     2761 ccatcgggga tgagctgcgc cggctgcgtc tggccgtcag ggaacagggg acgcgggatg
     2821 atagttaagt ttcatgccag gggaaaaggt ggtggcagtg gtccggttga ttacctgctg
     2881 ggaagggagc gtaaccggga aggggcaacg gtgcttcagg gtaatccgga agaagtccgg
     2941 gaactcatcg atgccacgcc atttgcgaag aaatacacgt ccggtgttct ctcgttcgcg
     3001 gagaaggcgc tgccagcggg aggacgtgaa aaagtgatgg cgagttttga gcgtgtactg
     3061 atgcccggtc tcgaaaaaaa tcagtacagc atcctgtggg tggagcacca ggacaaggga
     3121 cggcttgagc tgaattttgt cattccgaac atggagctac agaccggaaa acgcctccag
     3181 ccgtactacg accgggcaga caggtcgaga attgatgcct ggcagacgct ggtgaatcac
     3241 cattacgggc tgcatgaccc gaacgccccg gagaaccgcc ggacgctgac tctccctgat
     3301 aacctgccgg aaacgaaaca ggcgcttgct gagagcatta cacgggggat agacgctctt
     3361 tatcatgtcg gggagataaa agaccgtcag gatgtgattc aggcgctcac agaggcgggg
     3421 cttgaagtgg tcagggtgac gcgaagcagt atcagcattg cagacccgaa cggcgggaag
     3481 aatatcagac tgaaaggagc attctatgag caatctttta cagacgggcg cggagttcga
     3541 gaaaaagctg aaagagagag ccgaatctac agagaaaatg ctgaacgacg agttcaggaa
     3601 gctcgaagaa tctgtaagca aggctgtgac atcaaacgag acgaaaatca aagacgctat
     3661 agccctgttc acagcttcga cagaggaatc actgaaaaaa caccgggaag gggtgaaaga
     3721 ggcgatgatg cagcacagga aggacgtgtt aaagctggca gggaatacgg gcatgatgtt
     3781 gctggggatg gttattttcc tgtttaccgt gagtggcggg acgctctggt atcttggcgg
     3841 gatgatacag gcgaacctgg aagaaatcag gaagcagaac gagagtctgg agaagctgaa
     3901 cgcgaagaca tggggcgtgc gttatcacga ggggagcaac ggacgttttc tggtgttgcc
     3961 cgaggggatg aaagccgaaa cgaactggac gatggaaaac gggaaacaga atgccgtaag
     4021 gctggtacag gagtaacaga gcatgacgga gctggaaaga catttgctga acgcattaga
     4081 gcaactgcaa caggactata tgcagcggct ga
//
LOCUS       DAZPEB010000249         4084 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZPEB010000249 DAZPEB010000000
VERSION     DAZPEB010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4084
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.254"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            143..346
                     /gene="aaeX"
                     /locus_tag="ACHURJ_003884"
     CDS             143..346
                     /gene="aaeX"
                     /locus_tag="ACHURJ_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFR1282632.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            354..1286
                     /gene="aaeA"
                     /locus_tag="ACHURJ_003885"
                     /pseudo
     CDS             354..1286
                     /gene="aaeA"
                     /locus_tag="ACHURJ_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
     gene            1292..3259
                     /gene="aaeB"
                     /locus_tag="ACHURJ_003886"
     CDS             1292..3259
                     /gene="aaeB"
                     /locus_tag="ACHURJ_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFR1282633.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IIADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTGLQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQQRLLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            3351..3623
                     /locus_tag="ACHURJ_003887"
     CDS             3351..3623
                     /locus_tag="ACHURJ_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFR1282634.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(3679..3942)
                     /gene="yhcN"
                     /locus_tag="ACHURJ_003888"
     CDS             complement(3679..3942)
                     /gene="yhcN"
                     /locus_tag="ACHURJ_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFR1282635.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT          955 a          991 c         1073 g         1065 t
ORIGIN      
        1 agataacagc ataacagtta cgctaatata ttaaatatca atctacagca atgttgctct
       61 cgcccggctt tccatgccct tctctgcggc gacagatgct gaaaataata acgcctgctc
      121 tctctataca accaaggtca acatgagtct gtttcccgtt atcgtggtgt ttgggctgtc
      181 cttcccaccg atattttttg aattgctttt atcactggcg attttctggc tggtgcgccg
      241 ggtacttgtg ccaacaggta tctacgactt tgtctggcat ccggcgttgt tcaacaccgc
      301 gctctattgc tgcttgtttt atttgatatc gcgactgttc gtttgaggtt gaagtgaaaa
      361 cactaataag aaaattctcc cgtacggcca tcacggtcgt attagtcatt ctggccttca
      421 tcgcaatttt taatgcctgg gtctattaca ccgaatcccc ctggacgcgt gacgcgcgct
      481 ttagcgctga cgtcattgcg atcgcgccgg acgtttctgg actcattacc caggtgaatg
      541 ttcatgataa ccagctggtg aaaaaaggac agatactgtt caccatcgac cagccgcgct
      601 atcaaaaggc gcttgaggaa gcgcaagccg atgttgctta ttatcaggta ctggcacagg
      661 agaaacgcca ggaggccgga cgtcgtaacc gtctcggtgt gcaggcgatg tctcgcgaag
      721 agatcgacta ggctaacaac gtactacaaa cggttctgca tcagttagcg aaagcgcagg
      781 cgacccgcga tctggcaaaa ctggatcttg aacgcacggt gatccgcgcg ccagcagatg
      841 gctgggtgac caacctcaac gtctataccg gtgagtttat tactcgagga tcaacggcgg
      901 ttgcgctggt gaaacagaac tccttctatg tactggccta tatggaagaa actaagctgg
      961 aaggggtgcg tccggggtat cgtgcagaga tcacgccgct tggcagtaac aaagtgctga
     1021 aagggactgt tgatagtgtt gccgcagggg tcaccaacgc cagcagcacg cgtgacgaca
     1081 aagggatggc gactatagac tctaaccttg aatgggtgcg tcttgcgcaa cgtgttccgg
     1141 ttcgtattcg tctcgacaac cagcaagaga acatctggcc tgcgggcacc actgctacag
     1201 tggtggtcac tggcaaacaa gatcgcgacg aaagccagga ttcgttcttc cgtaaaatgg
     1261 cccatcgcct gcgtgagttt ggttaatcac gatgggtatt ttctccattg ctaaccaaca
     1321 tattcgcttt gcggtaaaac tggcgaccgc cattgtactg gcgctgtttg ttggctttca
     1381 cttccagctg gaaacgccac gctgggcggt actgacagcg gcgattgttg ccgccggtcc
     1441 ggcctttgct gcgggaggtg aaccgtattc tggcgctatt cgttatcgtg gctttttgcg
     1501 catcatcggc acatttattg gctgtattgc cggactggtg atcatcattg cgatgatccg
     1561 cgcaccatta ttgatgattc tggtgtgctg tatctgggcc ggtttttgta cctggatatc
     1621 ctcgctggta cgaatagaaa actcgtacgc gtgggggctg gccggttata ccgcgctgat
     1681 cattgtgatc accattcagc cggaaccatt gcttacgccg cagtttgccg tcgaacgttg
     1741 tagcgagatc gttatcggta ttgtgtgtgc gattatagcg gatttgctct tttctccgcg
     1801 atcgatcaaa caagaagtgg atcgagagct ggaaagtttg ctggtcgcgc aatatcaatt
     1861 aatgcaactc tgtatcaagc atggcgatgg tgaagttgtc gataaagcct ggggcgacct
     1921 ggtgcgacgc accacggggc tacaaggcat gcgcagcaac ctgaatatgg aatcttcccg
     1981 ctgggcgcgg gccaatcgac gtttaaaagc gatcaatacg ctatcgctga cgctgattac
     2041 ccaatcctgc gaaacttatc ttattcagaa tacgcgcccg gaattgatca ctgatacttt
     2101 ccgcgaattt tttgacacgc cggtagaaac cgcgcaggac gtccacaagc agctcaaacg
     2161 cctgcggaga gttatcgcct ggaccgggga acgggaaacg cctgtcacca tttatagctg
     2221 ggtcgcggcg gcaacgcgtt atcagcttct caagcgcggc gttatcagta acacaaaaat
     2281 caacgccacc gaagaagaga tcctgcaagg cgaaccggaa gtcaaagtag agtcagccga
     2341 acgtcatcat gcgatggtta acttctggcg aaccacactt tcctgcattc tgggcacgct
     2401 tttctggctg tggacgggct ggacttccgg cagtggtgca atggtgatga ttgcggtagt
     2461 gacgtcactg gcaatgcgtt tgccgaatcc acgcatggtg gcgatcgact ttatctacgg
     2521 gacgctggcc gcgctgccgt tagggctgct ctactttttg gtgattatcc ctaataccca
     2581 acagagcatg ttgctgctgt gtattagcct ggcagtgctg ggatttttcc tcggtataga
     2641 agtacagcaa cggctactgg gctcgatggg ggcgctggcc agcaccataa atattatcgt
     2701 gctggataac ccgatgactt tccatttcag tcagtttctc gacagcgcat tagggcaaat
     2761 cgtcggctgt gtgctcgcgt tcaccgttat tttgcttgtg cgggataaat cgcgcgacag
     2821 gaccggacgt gtactgctta atcagtttgt ttctgccgct gtttccgcga tgactaccaa
     2881 tgtggcacgt cgtaaagaga accacctccc ggcactttat cagcagctgt ttttgctgat
     2941 gaataagttc ccaggggatt tgccgaaatt tcgcctggcg ctgacgatga ttatcgcgca
     3001 ccagcgcctg cgtgatgcgc cgatcccggt taacgaggat ttatcggcgt ttcaccgaca
     3061 aatgcgccgc acagcagacc atgtgatatc tgcccgtagc gatgataaac gtcgtcggta
     3121 ctttggccag ttgctggaag aacttgaaat ctaccaggaa aagctacgca tctggcaagc
     3181 gccaccgcag gtgacggaac cggttcatcg gctggcgggt atgctccata agtatcaaca
     3241 tgcgttgacc gatagttagg tcaaaaccaa cgccaaaagt gtcggttttt tcatggctat
     3301 acttagcgat gcacggcaga actcgccgcg aaacgtgacg gtggcaatag atgaatattt
     3361 atacctttga ttttgatgag attgagagtc aggaggattt ttatcgtgac tttagccaaa
     3421 cctttggtct ggcgaaagat aaggtacgcg atctcgactc actatgggat gtgttaatga
     3481 acgatgtcct gccgctacca cttgagattg aatttgttca tctgggagag aaaacgcgtc
     3541 gccgttttgg cgcgttaata ttgctgtttg atgaggcaga ggaagagctg gaagggcatt
     3601 tgcgttttaa tgttcgtcat tagcgcaaaa aaaagccccc gaaccggggg caatatcgtc
     3661 ggacaagacg atgagggttt atttgtacag ttcagccgta gcgtgccagg tgtcaccgct
     3721 acgagcttca gtaatctggt aggccgttgc gcctttctct tccgcttttt tgttcagcat
     3781 ttcacgcata tccattggcg aagacgccac accacttacg gatacggtcc cgattgcttc
     3841 acgattttgt gcttgtgcag catcaatgga gtcggcagcg aatgcaccga aagagagaac
     3901 agaaagtacg cttaatgcag ctacagtggt tttgattttc atgattttta cctcgacata
     3961 atcttttagc tgggcctttg ttttgtgacc catatcacaa aatcaagcat acactaatta
     4021 ctgaactaat taataccacg ctaactgttt cagttgatac taagtgttaa aaatgtgaat
     4081 ttta
//
LOCUS       DAZPEB010000250         4080 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZPEB010000250 DAZPEB010000000
VERSION     DAZPEB010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4080
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.255"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            32..931
                     /gene="pgrR"
                     /locus_tag="ACHURJ_003889"
     CDS             32..931
                     /gene="pgrR"
                     /locus_tag="ACHURJ_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFR1282636.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            1268..2881
                     /gene="mppA"
                     /locus_tag="ACHURJ_003890"
     CDS             1268..2881
                     /gene="mppA"
                     /locus_tag="ACHURJ_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFR1282637.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRIMYIVKH"
     gene            complement(2932..3963)
                     /gene="ynaI"
                     /locus_tag="ACHURJ_003891"
     CDS             complement(2932..3963)
                     /gene="ynaI"
                     /locus_tag="ACHURJ_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFR1282638.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
BASE COUNT         1144 a          896 c          962 g         1078 t
ORIGIN      
        1 aaatgattaa tagattgcat ggagatactg aatgaagcgt gaagaaatcg ctgatctgat
       61 ggcgtttgtc gtcgttgcag aggagcgtag cttcactcgt gcagcagccc gcctgagcat
      121 ggcgcagtca gctttaagcc agatagtgcg tcgtatagaa gaacgattgg gattgcggct
      181 tctgacgcga accacgcgca gcgttgttcc aactgaagcg ggcgagcatc ttttgtctgt
      241 tcttggccct atgttgcatg acatagattc agccctggca tccctgagcg agctgcagaa
      301 ccgcccatcc gggacaatac gtattactac tgtagaacat gcagcaaaaa cgatattgtt
      361 accagcaatg cgcacattcc tgaaatcgca tcctgaaatt gatattcagc tcaccattga
      421 ttatggtttg accgatgtcg tttctgaacg ttttgatgca ggcgtccgtc tgggtgggga
      481 gatggataaa gatatgatcg ccattcgaat cgggccagat ataccaatgg ctattgttgg
      541 ctcaccggat tatttttctc gccgaagtgt tccaacgtca gtgtcacaat taatagatca
      601 tcaggcaatt aatttgtatc ttcccacatc gggtacagca aatcgctgga gattaatacg
      661 cggtggacgt gaagttcgtg ttcgcatgga aggtcagctt ttactgaata cgatagacct
      721 gatcattgat gctgcaattg atgggcatgg attggcgtat ctaccttatg atcaggttga
      781 gcgggctatt aaagaaaaaa aactgatacg tgttttggat aaattcacac cagatttacc
      841 cggttatcac ctgtactatc cacaccgtcg acatgctggc tcggcattct cattatttat
      901 agataggctg aagtataaag gtgctgttta gcactacttg ctgatacatt aatttaattt
      961 ctctcttaac gtattctcag ttcctttcaa cgttttggtt attttttatt cttcgtacaa
     1021 tggcgacaga tgctgatttt gataccgaaa actggtttga acgtgcgaag cctgaacgta
     1081 gtattcggtg ttctatgtgc tttgccgcat tttggcgtcg attatttaca ctaaatctga
     1141 tttaatatat tgatacataa aacattttat gcttcctgtg tcactttttt gatggaagtt
     1201 gtttgcattt ctttaaggcg aaacaaataa ttacgcatca attttaatgt cggttagagg
     1261 gaaacttatg aagcactctg tttcagtcac gtgttgtgcg ctgttggtca gcagcatttc
     1321 tctttcgtat gctgcagaag ttccgagcgg cacagtactg gcagagaagc aggagctggt
     1381 gcgccacatt aaagatgagc ctgcgtcgct ggatcccgct aaagccgtgg gcctgccaga
     1441 gattcaggtc attcgcgatc tgtttgaagg gctggtgaat cagaacgaga aaggggagat
     1501 tgtccccggc gttgcgactc agtggaaaag taatgacaac cgtatctgga cttttaccct
     1561 gcgcgataac gcaaaatggg cggatggcac accggtaacg gcgcaagatt ttgtctacag
     1621 ctggcagcgt ctggtggacc cgaaaacgct atcgccattt gcatggtttg ccgcgctggc
     1681 gggaatcaac aacgcacagg cgattattga tggtaaagct acgcctgacc agcttggcgt
     1741 caccgcagtt gatgcccata ccttgaaaat tcagcttgat aaaccgttgc cgtggtttgt
     1801 gaatttaacc gctaactttg ccttcttccc ggtgcaaaaa gccaacgtag aaagcggtaa
     1861 agagtggacg aaacccggaa atctgatcgg caatggcgct tatgttctta aagaacgcgt
     1921 agtcaatgaa aaactggtcg tggtaccgaa tacccattat tgggataacg ccaaaacggt
     1981 actgcaaaaa gtgaccttcc tgccaattaa tcaggaatcc gcagccacta agcgttacct
     2041 cgcgggggat attgatatca ccgaatcctt cccgaaaaat atgtatcaga agctgttgaa
     2101 ggatattccg gggcaggttt atacgccgcc gcagctcggg acctattatt atgcgtttaa
     2161 cacgcaaaaa gggccgacgg cagatcagcg cgttcgcctg gcattaagta tgacgataga
     2221 tcgccgcctg atgaccgaaa aagtgttggg aacgggcgaa aagccagcgt ggcattttac
     2281 accagatgtt accgccggat ttacgccgga accttcgccg tttgaacaaa tgagtcagga
     2341 agaactgaat gcgcaggcaa aaactttgtt gagcgcagct ggttatggtc cgcaaaaacc
     2401 gctgaagctg acgcttttgt ataacacttc agaaaaccat caaaaaattg cgattgctgt
     2461 agcatcgatg tggaaaaaga accttggcgt agatgttaaa ttgcaaaatc aggaatggaa
     2521 aacctatatc gatagccgta acaccggcaa ttttgatgtt atccgcgcct catgggtggg
     2581 ggattataat gaaccctcca ctttcctgac attattaacg tcaacgcatt caggaaatat
     2641 ttcacgcttt aacaatccgg catacgacaa agttctggcc caggcatcga cggaaaatac
     2701 cgttaaagcg cgtaatgccg attacaacgc ggcagaaaaa atcctcatgg agcaagcacc
     2761 gattgcacca atttatcaat ataccaatgg acgattaatc aagccgtggc tgaaaggtta
     2821 tcccattaat aacccggaag atgtggcgta cagccggatt atgtatattg tgaagcattg
     2881 atgtgatggg aactggcgtt acccttgtgg ataacgccag tgatgtctga tttagcgccc
     2941 ctgttccggc ggtgtaatgt tatccatata cagcgtctgg ctggggaagg caaagtccgc
     3001 gccgtgtgac tgcacaatat cgataatctt caaataaacg tcttgttgtg cagcaagcca
     3061 ttcagcccat accgtggttt tggtaaagca ataaaccata atattcaatg acgagtcggc
     3121 aaactggttg aaataaacca gtaaggtttg tcgctggtcg atggccgggt gatttttcag
     3181 catctcacgt acggcttcga caataacgcc cacttttgcc gcatcctcat aacgtaaacc
     3241 aatggtcgtg gtaatgcggc ggttggtcat tcgtcctggg ttttctacgc tgatcgacga
     3301 aaacagcgag ttcggtacgt acaatggacg attatcaaag gtcgtaattt tggtaattcg
     3361 ccagccaatt tccgctactg taccttcgat atttctgtcc ggtgaacgga tccagtcgcc
     3421 aatactgaaa ggacggtcga aatagagcat gatcccggaa aagaaattac tcagaatatc
     3481 tttaccggcc ataccgacag ccagaccacc aataccacca aaggtcagca agccagaaag
     3541 gctcatgccg aaatgttcgc cataaagcag aacaagcacc acaataatgg tgattttgat
     3601 aatgcgcgac ataatccgcg cactggtgat atcgcgacct tttttaatct gctgtttttc
     3661 aaactgatta atcagcaaaa atagcttaat cgtcagaata accgcaatca gggacgtaca
     3721 gataaaatcg ataacgcctg gggtgataaa tttgagttta tagttttcta taacataatt
     3781 aataatgcta ccaacagcac tgataattat ggtgtagatt aaaaattgca ccgcatggaa
     3841 taaaaaacct tttcttttac gatttccgcg gcgaaaccaa aagctcatca gaatcaatgc
     3901 tgcgcagcta ccgaaaataa tgaccagatt aagcgcatta tttgtaaaca gttcagcgat
     3961 cattgtttta tcaggctcct ccagataatt gtcgtcatgc cggaaccccc tggcggggct
     4021 attttaccgc gacaattcat tcagatcatc aatagtcagg gaaggaagta gcaacattag
//
LOCUS       DAZPEB010000251         4070 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZPEB010000251 DAZPEB010000000
VERSION     DAZPEB010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4070
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.256"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..1046)
                     /locus_tag="ACHURJ_003892"
     CDS             complement(<1..1046)
                     /locus_tag="ACHURJ_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR1282639.1"
                     /translation="MREINMLRNISSCLFPHISKITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
     gene            1537..1644
                     /locus_tag="ACHURJ_003893"
                     /pseudo
     CDS             1537..1644
                     /locus_tag="ACHURJ_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308311.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
     gene            1927..2922
                     /gene="pgl"
                     /locus_tag="ACHURJ_003894"
     CDS             1927..2922
                     /gene="pgl"
                     /locus_tag="ACHURJ_003894"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFR1282640.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTIEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHLISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(2963..3915)
                     /locus_tag="ACHURJ_003895"
                     /pseudo
     CDS             complement(2963..3915)
                     /locus_tag="ACHURJ_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
BASE COUNT         1091 a          830 c          952 g         1197 t
ORIGIN      
        1 aatgcccggg aattctcctc ggtggaaaaa gaagtccatt gtgtagaaac ctcccgacga
       61 tctgcttcag gaaaccaggc tgccacagtt tcgggaagag gggggatctg aaagttctgc
      121 cccccttcag tgatgactct gatatggcgc gaatgtaaat tttgcggcaa catctggcgt
      181 aaaaacagtc tgttttggtc tgacagggaa atacgaaact gaggcccgtt aaaatttgga
      241 accgaagttt gctgtattaa gaatagcaga gtttgatctg ataaggggtt atttgcaatc
      301 tcaatagtta tatgaggatc taaacgaaaa agatttgtcg ggattgtagt tatttggttg
      361 ttttcaaaat aaaaaaatct tctcatgata atattttctt ggatgtttgg taagttgctt
      421 aatctattgc tatatgcaaa aagaagttgc agtctcctag gtaaaggggg cagttcagta
      481 agatggttgt gcgcaacctt aagtatctta agattagaag gcagagatgg aagagtttgc
      541 agttcgttgt tcctaacctc aagttttttt aaattcaagg gtaatgaagg cagtgtggat
      601 aacctattat actctgcgct tagtgatttg atggtgtctg gtaataacgg cagattctct
      661 aattgatttt cggacacatc caaagttatt agataatgtg gtaatgcaga tatatgtgtt
      721 aagaaattat ctcttgcaat aagttcttta atattttcag gtaaatcagt aaggatggtt
      781 aatttattac agctcacatc aagcttttta atgcaagcag ggagtacagg taacgaggaa
      841 agcgccaagg ctcgcaggtc cagtttatgc cccttagagg taagacatgc acgaagtctt
      901 cttaccgcct cagtacgctg ttcttccggt aacccctgtt tctcccaatc atcccattcg
      961 gacaaataat ggttggggga tgtaattttg ctgatatgtg gaaataaaca ggatgaaata
     1021 tttctgagca tattaatttc tcgcatatgg atatggaatt agattgtgaa taaattgtat
     1081 ttaatgtatc tctcctaatg aggagggtgt ttcagaatat tcgccataag ggaatgagtg
     1141 ttatatcatg ctttctatcc tggtaagtat atttgctttt ttcgtatatt tggttcatct
     1201 ttttttgatg ttttacatat tcacaatgca gaggatttat accagaagac attagtcttg
     1261 agtaagatac tacaacatct ccaactattt tagccagcct gactacttga cttggtaata
     1321 tttgttgcta ataatcatgt ataaaataag cttatataca tagtaacttc agcaaaggat
     1381 ttagtaatga tccaaatcat atatcaatgt actgtttttt tctttaaaaa aacagtacat
     1441 tctattttat aatgactgtg tattcccatg gaaggagatc acccaattct cccccccgac
     1501 ttttgacgca gtgtcttgtt aacaggggga tttaccatgc ttattggcta tgtacgcgta
     1561 tcaacaaatg accagaacac cgatctacga cgtaatgcgt tgaactgtgc aggatatgag
     1621 ctgattgttg agcgaatggg agcttgattg gaagttgtta gggtatggtg tttacggtcg
     1681 ttgatatggc gaacaaacgc ggggagcgtc acgctcccta aatggtcgcg aaaatatctg
     1741 tggcattgtc aaatttgtga cggtgactaa tattttaatg atatgccatt cgaaatggtg
     1801 tgctaacgta ttgatttaat tgtgacgcga tgttgtattt tatcgcgttt tgttgtctgt
     1861 ttttcattcg cttgatgttg ctatttacat gaaaaataaa gaatttattg tttttgaggt
     1921 gtgtttatga agcaaacagt ttatatcgcc agccctgaga gccagcaaat tcacgtctgg
     1981 aacctgaatc atgaaggcgc actgacgctg acacaggttg tcgatgtgcc ggggcaggtg
     2041 cagccgatgg tggtcagccc ggacaaacgt tatctctatg ttggtgttcg ccctgagttt
     2101 cgcgtcctgg cgtatcgtat tgccccggac gatggcgcac tgacctttgc cgcagagtct
     2161 gcgctgccgg gtagtccgac gcatatttcc accgatcacc aggggcagtt tgtctttgta
     2221 ggttcttaca atgcgggtaa cgtgagcgta acgcgtctgg aagatggcct gccagtgggc
     2281 gtcgtcgatg tggtcgaggg gctggacggt tgccattccg ccaatatctc accggacaac
     2341 cgtacgctgt gggttccggc attaaagcag gatcgcattt gcctgtttac ggtcagcgat
     2401 gatggtcatc tggtggcgca ggaccctgcg gaagtgacta ccattgaagg ggccggcccg
     2461 cgtcatatgg tattccatcc aaacgaacaa tatgcgtatt gcgtcaatga gttaaacagc
     2521 tcagtggatg tctgggaact gaaagatccg cacggtaata tcgaatgtgt ccagacgctg
     2581 gatatgatgc cggaaaactt ctccgacacc cgttgggcgg ctgatattca tatcaccccg
     2641 gatggtcgcc atttatacgc ctgcgaccgt accgccagcc tgattaccgt tttcagcgtt
     2701 tcggaagatg gcagtgtgtt gagtaaagaa ggcttccagc caacggaaac ccagccgcgc
     2761 ggcttcaatg ttgatcacag cggcaagtat ctgattgccg ccgggcaaaa atctcacctc
     2821 atctcggtat acgaaattgt tggcgagcag ggactactgc atgaaaaagg ccgttatgcg
     2881 gtcgggcagg gaccaatgtg ggtggtggtt aacgcacact aaccgctgat ttaaccggcg
     2941 cagtctctcc tgcgccggtg tattaaccta tctcctgtaa cgcatgtctc tggcgttcga
     3001 cgatattggt ccacaaattg tctttatcgt cagtccacaa attaatcagc aaggcaaaaa
     3061 agcgttcagc tgccggagaa agcacggcat ctttgcggcg aataatcccc aatgtccgac
     3121 gtataacggg ttccaccagt gggataccaa taatcgaaga atagggcgca tggggcattg
     3181 ccagaccagg aagcgccgaa atccccagtc ctgcctccac tagtcctaat gacgtcgaaa
     3241 gatgacgcac ttcgtaaaac cagtccagct tccagggctt gtcggccagc tgttgttcta
     3301 tcaacagtcg gttgccgctg gaggagcgta cgccaatcat tttgtaaccc actaactcct
     3361 gccattctac aagctgcttt ttggccaatg gatggtcacg ccgacaggca gcacgaacgg
     3421 ctcgttgacc agtggggtaa aatcaatgga tgaatttgtg acgttgttca tgtttatgcc
     3481 aaagtctgat tcgttacaca gcaccgattc catgcaatta ttcgtgcctt gttccagaat
     3541 ccgcacttta atattgggat acagctcatt aaatttaccg attgccagcg gtaaaaaata
     3601 aaatactgcg gtcggaatac acgctaacgt caccatacca cgatgataag cattcatatc
     3661 acgaatatta aaaagcgttt catcgaattt ctttattaat tcccgcgcct ccgggagcaa
     3721 cctttttcct gcttttgtta acgtaacctt gcgagttgta cgctcaaata gttgaatatg
     3781 taaatcttcc tccatctttt ttattcttcg cgttaatgca ggttgcgtaa tattgagtaa
     3841 tttagcggca ttgttaaacg aactggactc tgccagtatg acaaatgcct tcatacttga
     3901 taattcatgc ttcattatta ctccggaaaa tggaagcgac gattttgggt ggctggccgt
     3961 taaaaatttt aactgcattt agccaactta aattaatgaa aaaatgttat taatcgttga
     4021 gctaaagtca ttagagatgc tttgccctta atgtaaccat atcgcaataa
//
LOCUS       DAZPEB010000252         4039 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZPEB010000252 DAZPEB010000000
VERSION     DAZPEB010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.257"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            146..577
                     /locus_tag="ACHURJ_003896"
     CDS             146..577
                     /locus_tag="ACHURJ_003896"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282641.1"
                     /translation="MTMNLHTPTTAPKAAPATSVSPLEQSGSPKTKFPEIKTVKTAVV
                     KLISDIEGNLDQRLVYREAVEGLCQEAANRITQCMHSRSTGQTLRDLRELLSVTLDTI
                     IVVEFEGEQTRLNYGGRSAEDVIAMLKALHLEGRKAWLEVE"
     gene            complement(609..1136)
                     /locus_tag="ACHURJ_003897"
     CDS             complement(609..1136)
                     /locus_tag="ACHURJ_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001776119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HFR1282642.1"
                     /translation="MRKTTLLVLAAALCVPAFATAAPVLTAGFSPSDGRPALEIVLGA
                     INNARQSIDVAAYSFTSKPVATALAGANRRGVAVRVVADEKANSDRYTAVTYLINQGV
                     PVHLNGRYAIMHNKFMVIDGKNVQTGSFNYTASAVSRNAENVLLIEDAPQLAEAYQRE
                     FNRLWDEGIPLNALY"
     gene            complement(1129..1875)
                     /gene="mobC"
                     /locus_tag="ACHURJ_003898"
     CDS             complement(1129..1875)
                     /gene="mobC"
                     /locus_tag="ACHURJ_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001776118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="HFR1282643.1"
                     /translation="MLISSYQERYARSTEKIRVLLNFLKEETYSDFQIIKLLFDFKSD
                     RPLYRLLEKVEKMGLIQKHVHESRASRISLWGITTDGLAVILTADDNIMPARFEPYRL
                     TGWSLDHHLDNQLVRLTLEKNGATGWINGDRSTFLSQYSVKHRPDGLISLPDGRLIAV
                     ETERRIKTKARYQSIMASHLLAISDKKWRYAFYVAPDEAKKRALKIIFDSIASVIVKN
                     QHVQLEAKHRDVFRFYTLDELKNLELDNYA"
     gene            complement(1890..3797)
                     /locus_tag="ACHURJ_003899"
     CDS             complement(1890..3797)
                     /locus_tag="ACHURJ_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001776117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HFR1282644.1"
                     /translation="MIFDKLNHLLSPIANAVLAFIAFLQAHQLALALLSGVMLPFIFS
                     AKHGGQEKPTFLQRILILLSMICFVFGSIAPVAVWFLQRIYGREDIASPPLLTWTMAL
                     LFTVAGFILHVVLRRLISPEFDKVKCGMVKKTSMERDRRTDVRRVKEILPVTTEYDPM
                     DYIDLNKGIFIGLSREGEPQYIPLKEWQTQHADIIGTTGAGKGVVTGILLYQSILADE
                     GVFVLDPKNDEWAPHLYKKACADAGKPFVLIDLNKQEYQMNLIDGITADHLEELFVAG
                     FSLAEKGEAADFYRIDDRKAARTAAQFVKDNPGATVRDIYNGDYVQGIGETIKAFHGK
                     TEELAMLNSINAPGGFALKEIFDKGGCCYIIGSMRNSKIITAQRMILVRLFQLAELRD
                     RVAGNIRPIAIFKDEVKYHLSKPALEGLGAARDKGVHILMAHQSIADLRDCPADLNGD
                     AVVGAVVENCKFKLVYKLQDPDTAEWIARMSGTILVDDESRKVTTTAVLTEKVDNERM
                     LRQAESYYIDSNMLQNLPKFVSYIFSQNALAFPSLISPLKVKKAPLELFTLPPVAVAT
                     VAEVKPVLDLDFDKTAPAVPAPDIIPVSATTTKKPSATPIPTPPVQPDEEWLMASQPA
                     PNDEIHSLLDL"
BASE COUNT          902 a         1111 c         1114 g          912 t
ORIGIN      
        1 tcagcttccg gtaatcgtcc cccgtcacaa cctggacagc aagggtaaat ggcacgctgc
       61 gcgcccttgc tgaccagaac gactccgggc aggccgaacc gtcaggcgat acgaagacga
      121 aattcacact gacggagaaa cgaccatgac gatgaacctg cacaccccga caaccgcccc
      181 taaagctgca ccggcgacca gcgtatcccc gctggagcag tcaggctccc caaaaacgaa
      241 atttcctgaa atcaaaaccg tcaaaacggc ggtggttaaa ctgatcagcg acatcgaggg
      301 caacctcgat cagcgtctgg tatatcgcga ggccgtagag ggattgtgcc aggaggcggc
      361 caaccggata acccagtgca tgcattcacg cagtacaggg cagacgctac gtgatctccg
      421 cgagctgctg tcggtgacgc tggacaccat tatcgtggtg gagtttgaag gggagcagac
      481 ccgtctcaac tatggcggac gttcagcaga agacgtgatc gccatgctga aagcgctgca
      541 tcttgaagga cgcaaagcct ggctggaagt ggagtaaaaa aaaagccccg tccgcagacg
      601 gggctgattc agtacagggc attcagcggg atgccctcat cccacaggcg gttaaattct
      661 cgctggtacg cctccgccag ctgcggcgca tcctcgatca gcagcacgtt ttccgcgttg
      721 cgcgaaacgg cgcttgccgt gtagttaaac gacccggtct ggacgttttt gccgtcgatc
      781 accataaact tgttgtgcat gatggcgtaa cggccgttaa ggtgcaccgg cacgccctgg
      841 tttatcagat acgtcacggc ggtgtagcgg tcgctgttcg ccttttcgtc ggccaccacg
      901 cgcacggcca cgccgcgccg gttagcaccg gcgagcgccg tcgcgaccgg cttactggtg
      961 aagctgtagg ccgcgacgtc aatgctctgc ctggcgttgt ttatggcgcc gagcacgatt
     1021 tcaagagcag ggcgcccgtc agacggcgaa aaacccgccg tcaggacggg cgcggcggtg
     1081 gcgaacgcgg gcacgcagag ggcggcggcc agcaccagaa gggtggtttt acgcatagtt
     1141 gtccagctcc aggtttttca gttcatcgag ggtgtaaaag cggaaaacgt cgcggtgttt
     1201 tgcctccagt tgcacatgct ggtttttcac gatcacgctg gcgatgctgt cgaaaataat
     1261 cttcagggcg cgctttttcg cctcgtccgg ggcgacgtaa aacgcgtacc gccatttttt
     1321 atcgctgata gccagcaggt ggctcgccat gatggactga tagcgcgcct ttgttttgat
     1381 acgtcgttcg gtttcaacgg cgatcagcct gccgtccggc aggctgataa gcccgtcggg
     1441 acggtgtttg acgctgtact gactgaggaa cgttgaacgg tcgccgttaa tccaccctgt
     1501 cgcgccgttc ttctccagcg taaggcgcac tagctggtta tcaaggtgat ggtcgagtga
     1561 ccagccggtc aggcggtagg gttcaaagcg ggcgggcatt atgttgtcgt ccgccgtgag
     1621 gataacggcc agcccgtcgg tggtaatgcc ccacagggat attctcgacg cgcgggattc
     1681 atgcacgtgc ttctgaatca gccccatttt ctccaccttc tccaacaacc ggtagagagg
     1741 gcggtcagat ttaaaatcaa aaagcagctt tatgatttga aaatcgctgt aggtttcctc
     1801 cttgagaaaa ttcagcagta cgcgaatttt ttcagtgctg cgtgcataac gttcctgata
     1861 cgacgaaata agcatagaaa ctccttattt tataaatcca gcaacgaatg tatttcgtca
     1921 tttggagcgg gctgtgatgc catcagccac tcctcgtcag gttgcaccgg cggagtgggt
     1981 attggcgttg ctgagggttt tttggtggtt gttgcgctga ccggaataat gtcaggtgcc
     2041 ggaactgccg gagccgtttt atcaaaatcc agatccagca ccggtttaac ttctgccacc
     2101 gtagcgactg cgaccggcgg cagggtaaac agctccagtg gggctttttt aaccttgagc
     2161 ggtgagatta acgacgggaa cgcgagcgcg ttctggctga agatatagct gacgaacttc
     2221 ggcaggttct ggagcatatt gctgtcgatg taatagcttt ccgcctgccg aagcatgcgc
     2281 tcgttatcga ctttttctgt cagcaccgcc gtggtggtga ctttccggct ttcatcatcg
     2341 actaaaatcg tgcccgacat gcgcgcaatc cattcggcgg tgtctggatc ctgaagttta
     2401 tataccagct taaacttgca gttttccaca accgccccaa ccacggcgtc gccgtttaaa
     2461 tccgccggac agtcgcgcag gtcggcaatt gactggtgcg ccattaatat atggacgcct
     2521 ttatcgcgtg ccgcgcctaa cccttcgagc gccggtttgg acaggtgata tttcacctcg
     2581 tccttaaaga tggcgatggg gcggatattc cccgcgacgc ggtcgcgcaa ttccgcgagc
     2641 tggaacaggc gaactaaaat catgcgctgc gcggtaatga ttttagagtt acgcatcgaa
     2701 ccgatgatat agcagcagcc gcccttgtcg aaaatctcct tcagcgcaaa accgcctggc
     2761 gcgttaatgc tgttgagcat tgccagctcc tcggttttac cgtgaaaggc tttaatcgtc
     2821 tcgccaatgc cctgaacata atcgccgtta taaatatccc gcacggttgc gcccggatta
     2881 tccttcacga actgcgccgc cgttctcgcc gccttgcggt cgtcgatacg gtaaaaatcc
     2941 gccgcctcgc ctttttccgc cagactgaaa ccggccacga ataattcctc cagatggtcg
     3001 gcggtgatac cgtcgataag gttcatctgg tattcctgtt tattcaggtc gataagcaca
     3061 aagggttttc cggcgtccgc gcacgctttt ttatacaggt gcggtgccca ctcgtcgttt
     3121 ttggggtcga gaacaaaaac gccctcgtcg gcgaggatgc tctgataaag cagaataccg
     3181 gttacgacgc ctttaccggc accagtggtg ccgataatgt cggcgtgctg ggtctgccat
     3241 tcttttaacg gaatatattg cggctcaccc tcgcggctca gcccgataaa aatcccctta
     3301 ttcagatcga tgtaatccat cggatcgtat tctgtggtga cgggtaaaat ttcttttacc
     3361 cggcgaacgt cggttctgcg gtcgcgctcc atgctggttt ttttcaccat gccgcatttg
     3421 accttatcaa attcaggcga gatcaaacgc cgtaaaacca cgtgcagaat aaaaccggcg
     3481 acggtaaaca gtagcgccat tgtccaggta agcagcggcg gactggcgat atcttcccgg
     3541 ccataaatgc gctgtaaaaa ccagacggcc actggcgcta tcgagccgaa aacaaaacag
     3601 atcatggaaa gcagaattaa gatcctttgc agaaacgtcg gcttttcctg accgccgtgt
     3661 ttcgcagaaa atataaaagg cagcatgacg ccggacagca gcgctaacgc cagttgatgc
     3721 gcctgcaaaa aggcgataaa ggccaggacg gcatttgcta tcggcgacag caaatgattc
     3781 agtttgtcga atatcacgat attctccttc acttaaattc ggtcagcacc atcggtcagt
     3841 cagaacggtc acaggttgtg atcgttctga ctgaccgatt ccatgctgca aaagaagtgg
     3901 ccgctaagcg gccatgatcg caggaggtac actcccgcga tctaacggcg tgcggcatta
     3961 cgcgcgggct ggacgccctt actgcatgtc gcatgggggt aatgtcctgg ctgaacgccc
     4021 ggccaaaacc cccaaggtc
//
LOCUS       DAZPEB010000253         4023 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZPEB010000253 DAZPEB010000000
VERSION     DAZPEB010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4023
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.258"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..377
                     /locus_tag="ACHURJ_003900"
     CDS             <1..377
                     /locus_tag="ACHURJ_003900"
                     /inference="COORDINATES: protein motif:HMM:NF019069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TrbM/KikA/MpfK family conjugal transfer protein"
                     /protein_id="HFR1282645.1"
                     /translation="PTLNNQRALAPCFPSEEIIMKHTVITAALLCALTSPAFAADGQN
                     ADPNDPCAMVLCLAGKLDGSSPAECDPMYKNFMSIKKKKKGSFLPDHTAAARQKKLSA
                     CPSADSAIVGKIISKFGRMNGW"
     gene            404..739
                     /locus_tag="ACHURJ_003901"
     CDS             404..739
                     /locus_tag="ACHURJ_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282646.1"
                     /translation="MINQTVSINNFSGLLKDEELPAGDLQTVLLVLSEISQKQDHFQA
                     NTSELVQLAAQHSPTFQALPAERQSFFRSVLSMPVFFYTVPLSDDEAAEWEKLNDKDA
                     LQWQQKPDL"
     gene            715..1023
                     /locus_tag="ACHURJ_003902"
     CDS             715..1023
                     /locus_tag="ACHURJ_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282647.1"
                     /translation="MATKTGFIAPPWPHVRGDSVSAASLISRIEKEAHRGCGLHYEIY
                     QYRALCELHDVLTSLSVRDAETFMTVAADHGFSLSDNDFQESRMMYFETMDEIRRDQE
                     "
     gene            1020..3221
                     /locus_tag="ACHURJ_003903"
     CDS             1020..3221
                     /locus_tag="ACHURJ_003903"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HFR1282648.1"
                     /translation="MRLWIAEKPAVASDIAKALGGNFTRHDGYAESATDIVSYCVGHV
                     LEMVPPETINPAYATWSLDTLPLKLYPVQLQPKASVAKQANTLVKLIRRPDIKMVVHC
                     GDPDDEGQLLVDEVLEFAGNTAPVKRALINDNTAPAVKKAINSLRDNSDFRGMYLKAL
                     MRSAGDAIFGFSMSRCYTLKARDKGYKGTISVGRVQTPVLGMIVRRWRDNQAHSEAFY
                     YQLAGQFISGTDVVCARWQTSEYAPVDDKKRLTDKAWGEGLARALAGKPASVLAAATD
                     RGKTTAAPLPFNLLRLQVYMNRRHKLTAQQTLDITQKLKDSKYITYNRSDCSYLSDEQ
                     FNDAPQVVAALKTLDVFNGLTTDTAQKSAAFDSKKVTAHTAIIPTTNMPDLSRLTDKE
                     KAVYLTIAQFYLAQFVAKKRYDESIAEIKCGDEMFKVSARKITDAGFTTFLNDAEEDD
                     EEDENSTSFEAISRLQTGATLTCREVVISEKKTKPLPLFTEATLLAALVRVADFVADP
                     RIKKLLKEKDKDKKDEHGGIGTPATRAAIIETLKTRDYITVEKGKFIPTAAGLALIDA
                     LPDSVTQPDMTAVWAEKQAAVETGEMTIERFIDGLYAEVSRLVESADIAISATEKHPV
                     KTALNRLAVKCPSCGSELVMTPKTCACTGCQFKIWLEVRGKKLTEKQAEKLIGKGKTD
                     VIKGFKSKKNDDTYSMIVTLKNAQTGELGFEYPPRDPLKGLNVSMPKELKL"
     gene            3218..3610
                     /locus_tag="ACHURJ_003904"
     CDS             3218..3610
                     /locus_tag="ACHURJ_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS family nucleoid-associated regulatory
                     protein"
                     /protein_id="HFR1282649.1"
                     /translation="MNRIQDELTSERALKKFLRESDFAFLEMLSARISAALDEKRAEH
                     IEQEKLRRQREEKRNELLALIESEGFSPTELVSTTPAKKSAKRKAKYQYFENGVHKKW
                     SGVGRVPVVIQQELDAGKPLESFLITTD"
BASE COUNT         1039 a         1085 c         1114 g          785 t
ORIGIN      
        1 caccgacgtt gaacaaccaa cgggcgttag cgccctgctt cccttcagag gaaatcatca
       61 tgaaacacac cgtaatcacg gcggcgctgc tgtgcgccct tacttctccg gcgttcgccg
      121 ccgatggcca gaatgccgac ccgaatgacc cgtgcgcgat ggtgctgtgc ctggccggaa
      181 agctcgacgg cagcagtccc gccgagtgtg acccgatgta caaaaatttc atgagcatca
      241 aaaagaaaaa aaaggggtcg tttttacccg atcacaccgc cgcagcgcgg cagaaaaaac
      301 tgagcgcctg cccgtccgct gacagcgcca ttgtcggcaa gatcatctcg aaatttggcc
      361 gtatgaacgg ttggtaaaac ggcagccgta aaggagacac cacgtgatta atcaaaccgt
      421 ttctattaat aacttttccg ggctgctgaa agatgaggag cttcctgcag gtgatttgca
      481 gacggtgctg ctcgttttgt cggaaatcag ccagaaacag gatcattttc aggccaacac
      541 gtccgaactc gtccagctcg ccgcgcagca ctcgccgacc ttccaggcat taccagcaga
      601 acgtcagtcg ttcttccgct ccgtgctcag tatgccggtc ttcttttaca ccgttccgct
      661 gagcgacgac gaggcggcgg aatgggaaaa actcaacgat aaggatgcac tccaatggca
      721 acaaaaaccg gatttatagc cccgccgtgg ccgcatgtgc gtggtgattc cgttagtgcc
      781 gcgagcctga tttctcgcat cgagaaagaa gcgcatcgcg gctgcggcct gcattacgaa
      841 atctaccaat atcgcgccct gtgcgaattg cacgatgttc tgaccagtct cagcgtccgt
      901 gatgctgaga cgttcatgac agtagcggcg gatcatggct ttagtctgtc tgataacgac
      961 tttcaggaaa gtcgaatgat gtacttcgaa acgatggatg aaatcaggag agatcaagaa
     1021 tgagactgtg gattgcagaa aaacccgcag tcgcgagcga tatcgcgaaa gcgctgggcg
     1081 gcaacttcac ccggcacgac ggctatgccg aatcggcgac cgatatcgtg agctactgcg
     1141 tcggccacgt gctggaaatg gtgcccccgg agacgatcaa cccggcctac gccacctggt
     1201 cgctcgacac cctgccgctg aagctgtacc cggtacagct tcagccaaaa gcgagcgtgg
     1261 caaagcaggc taataccctg gtgaagctga ttcggcgccc ggatatcaaa atggttgtcc
     1321 actgcggcga cccggacgac gaggggcagc tcctggttga cgaggtgctg gagttcgccg
     1381 gaaacaccgc gccggtgaaa cgggcgctta ttaacgacaa caccgcgcca gcggtgaaaa
     1441 aagccatcaa ctccctgcgc gataacagcg attttcgtgg gatgtacctg aaagccctga
     1501 tgcgcagcgc aggagacgcg atttttggct tcagtatgag ccgctgttac accctcaaag
     1561 cccgcgataa gggctataag ggcacgattt cggttggccg cgtccagacg ccggtgctgg
     1621 gaatgattgt gcgccggtgg cgcgacaatc aggcgcacag cgaggcgttt tactaccagc
     1681 tcgcagggca gtttatcagc ggtacggacg ttgtctgcgc ccgctggcag acgtccgaat
     1741 acgcgccggt ggacgataaa aagcggctga cggataaggc ctggggggaa ggactcgcga
     1801 gggctctggc cggaaaaccg gccagcgtgc tcgcggccgc caccgaccgg ggaaaaacca
     1861 cggccgcacc gctgccgttt aacctgctgc gcctccaggt gtatatgaac cgcaggcaca
     1921 aactgaccgc gcagcagacg ctcgatatca cgcagaagct caaggacagt aaatacatta
     1981 cctacaaccg ctctgactgt tcttatctgt ccgatgaaca attcaacgac gcgccacagg
     2041 tggtggcggc cttaaaaacg ctcgacgttt ttaacggcct taccaccgac acggcgcaaa
     2101 aaagtgcggc gtttgacagt aaaaaagtca ccgcccacac cgccattatc ccgacgacga
     2161 acatgccgga tttaagccgc cttaccgaca aggaaaaggc ggtttatctc accatcgcgc
     2221 agttttatct cgcgcagttc gtggcgaaaa agcgctatga cgagtcgatc gcggagatta
     2281 aatgcggtga cgagatgttt aaagtctcgg cgcgtaaaat caccgatgcg ggttttacga
     2341 cgttcctgaa cgacgcggaa gaagacgacg aggaggacga aaacagcacc tcgtttgagg
     2401 ccatcagccg cctgcaaacc ggggcgacat taacctgccg cgaggtggtg atttcggaga
     2461 aaaaaactaa accgctgccg ctgtttaccg aggcgaccct gctcgctgcc ctcgttcgcg
     2521 tggccgattt tgtcgccgat ccgcgcatta aaaaattgct caaggagaag gataaagaca
     2581 aaaaagatga acacggcggc atcggcacac cggcgacccg cgcggctatt attgagacgc
     2641 tgaaaacccg cgattacatc accgtggaaa agggcaaatt tatcccaact gccgccgggc
     2701 tggcgttaat tgatgccctg cccgactcgg tgacgcagcc ggatatgacc gccgtatggg
     2761 ctgaaaaaca ggcggcggtt gaaaccggcg aaatgaccat cgagcgcttt attgatggtc
     2821 tttacgcgga agtttcccgc ctggttgaga gcgccgatat tgccatcagc gcgacggaaa
     2881 aacatcccgt taaaacggcg cttaaccgcc tcgcggttaa atgtccgtcg tgcggttctg
     2941 agctggttat gacgccgaaa acctgcgcct gtacgggctg tcagttcaaa atatggctgg
     3001 aagtgcgcgg caaaaagctg acggaaaaac aggccgagaa gttaatcggc aagggcaaaa
     3061 ccgacgttat taaaggtttt aaatcgaaga aaaacgacga cacctattcg atgatcgtca
     3121 cgctgaaaaa cgcgcaaacc ggggaactcg gttttgaata tccaccacgc gatccgctga
     3181 aaggattgaa cgtatctatg ccgaaggagc tgaaattatg aaccgtatcc aggacgaatt
     3241 aacctcagag cgcgcgctga aaaagttttt acgtgaaagt gattttgcct ttctggagat
     3301 gctgagtgca cgtatttcag cagcgcttga tgaaaaacgc gcagagcata tcgagcagga
     3361 aaagctacgc cgacagcggg aagaaaagcg caatgaattg ctggcgctga ttgaatcgga
     3421 gggcttttca ccgacagagc tggttagtac aactcccgcc aaaaagtcgg caaaacgcaa
     3481 agctaaatat cagtattttg aaaacggcgt gcataaaaaa tggtcaggcg tcggacgcgt
     3541 gccggttgta attcagcaag aacttgacgc cggaaaacct cttgaatcct tccttattac
     3601 caccgactaa caacggcaca gggtcggttt tgacctggct tttcgggctt cgaagactgg
     3661 cgagcatggc gattcaattt tgaaaaaatt gctaatcgac aggccgcctg agtctgcgaa
     3721 gtggcaggag gcgagtaaat tcaatttcga agaaattgct aatttgcgtc gccgattcca
     3781 ctttttgagc gctcacccca agtgagccga aaaaagccgg ggggggccga ataaattttg
     3841 ccgccccagc gagcaaaaag acgcgcccac gcgtcggttt atttgggggg cttgcccccc
     3901 acaccgtcgt gcctaagtct ccggccaaaa gccggtagcg tggcgcgacc accccctaaa
     3961 aagcctagtt tccctttaat tcgtcttgtg aggaacgaac aaggcgaaga aaagggctaa
     4021 aac
//
LOCUS       DAZPEB010000254         3978 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZPEB010000254 DAZPEB010000000
VERSION     DAZPEB010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.259"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(344..577)
                     /locus_tag="ACHURJ_003905"
     CDS             complement(344..577)
                     /locus_tag="ACHURJ_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFR1282650.1"
                     /translation="MRIEIMIDKEQKISQSTLDALESELYRNLRPLYPKTVIRIRKGS
                     SNGVELTGLQLDEERKQVMKIMQKVWEDDSWLH"
     gene            complement(588..776)
                     /locus_tag="ACHURJ_003906"
     CDS             complement(588..776)
                     /locus_tag="ACHURJ_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282651.1"
                     /translation="MQDYFLESLKLQRIDFFLKLVAASECSDEEKGLALQWVSELTDE
                     LMAKIRTHEYNRSMDVIS"
     gene            complement(930..3344)
                     /locus_tag="ACHURJ_003907"
     CDS             complement(930..3344)
                     /locus_tag="ACHURJ_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFR1282652.1"
                     /translation="MSHADMNNCCGFNEAAAAFSWNSPKKAINPYLDPAEVAPVSALS
                     NLITLYAADNEQEQLRREALSDQVWERYFFYESRDPVQHEMEQDKLISRAKLAHEQQR
                     FNPDMVILADVNAQPSHISKPLMQRIEYFSSLGRPKAYSRYLRETIKPCLERLEHVRD
                     SQLSASFRFMASHVGLDGLLILPEMSQDQVKRLSTLVAAHMSMCLDAACGDLYATDDV
                     KPEEIRKTWEKVAAETLRLDVIPPAFEQLRRKRNRRKPVPYELIPGSLARMLCADWWY
                     RKLWKMRCEWREEQLRAVCLVSKKASPYVSYEAVMHKREQRRKSLEFFRSHELVNEEG
                     DTLDMEDVVNASSSNPAHRRNEMMACVKGLELIAEMRGDCAVFYTITCPSRFHSTLNN
                     GRPNPTWTNATVRQSSDYLVGMFAAFRKAMHKAGLRWYGVRVAEPHHDGTVHWHLLCF
                     MRKKDRRAITALLRKFAIREDREELGNNTGPRFKSELINPRKGTPTSYIAKYISKNID
                     GRGLAGEISKETGKSLRDNAEYVNPWASLHRVQQFRFFGIPGRQAYRELRLLAGQAAR
                     QQGDKKAGAPVLDNPRLDAILAAADAGCFATYIMRQGGVLVPRKYHLIRTAYEINEEP
                     TAYGDHCIRIYGIWSPIAEGKICTHAVKWKMVRKAVDVQEAAADQGACAPWTRGNNCL
                     LAENLNQQEKDKSADGNPRTDITSMDDKELHDYLHSMSKKERRELAARLRLVKPKRRK
                     DYKQRITDHQRLQLVYELKSRGFDGSEKEVDLLLRGGSIPSGAGLRIFYRNQRLQEDD
                     QWRNLY"
     gene            complement(3341..>3978)
                     /locus_tag="ACHURJ_003908"
     CDS             complement(3341..>3978)
                     /locus_tag="ACHURJ_003908"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HFR1282653.1"
                     /translation="RVLFEIANREVAYYNIRQEFNYSTEITDFMKAVYFLYLNRHGYR
                     GLCRYNKSGHFNIPYGNYKNPYFPEKEIRAFAEKAQRATFICASFDETLAMLKAGDVV
                     YCDPPYDGTFSGYHTDGFTEDDQYHLASVLEHRSSEGHPVIVSNSDTSLIRSLYRNFT
                     HHYIKVKRSIGVAAGEGKSATEIIAVSGPRCWVGFDYSRGVDSSAVYGVRA"
BASE COUNT          963 a         1067 c          901 g         1047 t
ORIGIN      
        1 atttgtaatt aaaaaatata aactataatt tttatttttc ttgactaaca ttattaccct
       61 ctcacttcat tagtgatgat ttattttgga attattttac acacttatac ctagcgcgca
      121 atcgtatccc cgccacgcct gcccgcttta tgtagaggtt ttcatgcacc tgcatgatct
      181 acgcaaaagc ccgccagttc tggcgggcct tagcaaaaac gatcctcaaa cgatcatgcg
      241 atctcatgcg gcatagacat gcactacaga gctaacgcct cgcaagggct cgttgttcaa
      301 ccttgctgac gccagaagct agttcagacg ccagcaacgt ttcttaatgc agccagctgt
      361 cgtcttccca caccttctgc ataattttca tcacttgttt tctttcttcg tccagttgca
      421 atccagtaag ttccacaccg ttagagctac ctttgcggat acgaattacc gttttgggat
      481 acagagggcg cagattgcgg taaagctcgg attcaagagc gtccagggta gactggctaa
      541 tcttctgctc tttatcgatc attatttcaa tgcgcataaa agtcacctca gctgatgaca
      601 tccattgagc ggttgtattc gtgggttctg atttttgcca tgagttcatc agtcaattca
      661 gaaacccact gcagagccag ccccttctct tcatcactac actcactagc cgctacaagc
      721 ttaagaaaaa aatcaatgcg ctggagcttc aaagactcca aaaaatagtc ctgcatcttt
      781 cctcctatga caccacacgc aatactgtat gcataaccac tgtttatatt tacagtatat
      841 aataatctta ctgatgtaaa acgttttttt acgttcatca gcctgatatg cctggtatta
      901 ttaagagcac gaattgttaa cccgcgtaat taatacaggt tccgccactg atcatcttcc
      961 tgcaaacgct ggttccgata gaagatacgc aggcctgctc ctgacggaat actgcctccg
     1021 cgaaggagta aatcgacctc tttctcgctg ccatcaaatc ctctggactt cagctcatac
     1081 acgagctgca gtcgctgatg gtctgtaatt cgctgtttgt agtctttacg ccgtttcggt
     1141 ttcaccaggc gtaaccttgc tgccagttcc cggcgctctt ttttgctcat actgtgcagg
     1201 taatcgtgca actccttgtc atccatgctg gtaatgtccg ttctggggtt cccatcagct
     1261 gatttatctt tctcctgttg gttcaaattt tcagcaagga gacagttatt gccacgagtc
     1321 caaggggcgc aagcgccctg gtcggctgcc gcctcctgaa cgtcaacggc tttacgaacc
     1381 attttccact tcactgcatg agtgcagatc ttgccttctg caatgggtga ccagatgcca
     1441 taaatacgaa tacagtgatc gccataggcg gtcggctctt cattgatttc ataagcggtt
     1501 ctgatcaagt gatatttgcg aggaaccagc acgccgccct gcctcataat gtaggtggca
     1561 aaacagccag catcagcagc agccaggatg gcatcaaggc gcgggttatc cagtaccggc
     1621 gcacctgctt ttttgtcacc ctgctgcctt gccgcctgac cagccaacaa ccgcagttca
     1681 cggtaagcct gacgccctgg aataccaaag aagcggaatt gctgaacacg atgcagagac
     1741 gcccagggat taacgtattc agcgttatca cgcagggatt tacccgtttc cttgctgatc
     1801 tcgccagcca gaccacgccc gtcaatgttc ttactgatgt atttcgcgat gtagcttgtc
     1861 ggcgttcctt tgcgcgggtt tatcagctca gatttaaagc gtggaccagt gttattaccc
     1921 agttcctcgc ggtcttcacg gatggcaaac ttacgcaaca atgcagtaat ggcgcggcgg
     1981 tcttttttgc gcataaaaca caacaggtgc cagtgaactg taccgtcatg atgcggctca
     2041 gccacccgca cgccatacca gcgcaacccg gctttgtgca tcgccttacg aaatgcagca
     2101 aacatgccga ccagataatc gctgctttgt cttaccgtcg catttgtcca ggttgggttt
     2161 ggtctgccgt tattgagcgt ggaatggaaa cgtgacggac aggtaatggt gtagaaaacg
     2221 gcgcagtcac cgcgcatttc cgcgataagc tccagacctt taacacaggc catcatctca
     2281 ttgcggcggt gcgccgggtt gctgctgctg gcgtttacca cgtcttccat atccagcgtg
     2341 tcgccctctt cgttcaccag ttcatgagaa cggaaaaact ccaatgactt gcggcgctgc
     2401 tcacgtttat gcatcacggc ttcatagctg acatagggag atgctttttt gctgaccagg
     2461 cagacagcac gcaactgctc ttcccgccat tcgcaacgca ttttccataa tttccggtac
     2521 caccagtcgg cgcacaacat acgcgccagc gaacccggaa tgagttcata gggcacgggt
     2581 ttacggcggt ttcttttccg acggagttgc tcaaacgcag gcgggatgac atccagacgc
     2641 agggtttccg ctgccacctt ttcccatgtc ttgcggattt cttctggctt aacgtcatcg
     2701 gtggcataca aatcaccaca agctgcatca aggcacatgc tcatatgcgc agcgacaagg
     2761 gtagacaggc gtttcacctg atcctgactc atttcaggca gaatcagcag gccgtccagc
     2821 cctacatggc ttgccataaa acgaaaagat gcagatagct ggctgtcgcg tacatgctcc
     2881 agtcgttcca gacatggctt aatcgtctca cgtaaatagc gggaataagc ctttggcctg
     2941 cccaggctgc tgaagtattc aatacgttgc atcagcggct tgctgatatg ggaaggctgg
     3001 gcgttgacgt ccgccagaat gaccatatcc ggattaaaac gctgctgctc atgcgccagc
     3061 tttgcccggc taatgagctt atcctgctcc atttcgtgct ggacaggatc acgtgattca
     3121 taaaaaaaat aacgctccca gacctgatca ctcagtgcct cgcggcgcaa ctgttcctgc
     3181 tcgttatcgg cagcgtacag agtgatcagg tttgaaagcg cagaaaccgg cgcaacttcc
     3241 gccgggtcca gataagggtt aatggccttt ttcgggctgt tccatgagaa cgctgcggca
     3301 gcctcgttaa agccgcagca gttgttcata tcggcatggc tcatgcacgt actccgtaca
     3361 cggcagaact atccacgcca cgcgaataat caaatcccac ccagcagcgc ggcccggaaa
     3421 cagcaatgat ttctgttgct gatttaccct cgccagctgc cacaccgatg ctgcgtttta
     3481 ccttgatata gtggtgagta aaattgcgat acagcgaacg gatcagggat gtgtcactgt
     3541 tagaaacaat gaccggatgt ccttctgatg accgatgttc aagaacggat gctaggtgat
     3601 actggtcatc ttcagtgaaa ccatcagtgt gatagccgga aaacgtaccg tcatacggtg
     3661 gatcgcaata caccacatcc cccgccttca acatcgccag cgtttcatca aagctggcgc
     3721 agataaacgt tgctcgctgg gctttttctg caaatgcgcg aatttctttt tcagggaaat
     3781 acggattttt ataattgccg tagggaatgt tgaaatgccc gctcttgtta tagcgacata
     3841 aaccacggta accgtgacga ttgagataca ggaaatatac cgctttcatg aaatcagtaa
     3901 tttcagttga gtaattaaac tcctgcctta tgttgtaata agccacctcc ctgtttgcga
     3961 tctcaaataa aactctgg
//
LOCUS       DAZPEB010000255         3914 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZPEB010000255 DAZPEB010000000
VERSION     DAZPEB010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3914
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.260"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            285..626
                     /gene="raiA"
                     /locus_tag="ACHURJ_003909"
     CDS             285..626
                     /gene="raiA"
                     /locus_tag="ACHURJ_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFR1282654.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     gene            complement(777..1583)
                     /locus_tag="ACHURJ_003910"
     CDS             complement(777..1583)
                     /locus_tag="ACHURJ_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFR1282655.1"
                     /translation="MTVGIAIQHQPAHTPEHTKVYQTDNVTLCRGNALEILPLIEPES
                     IDALITDPPYSSGATHKAGRTNQGSHAKYLNGENLHRFDGFAGENMDARSWAYWTQLW
                     MAQAHRAVRPGGYALVFTDWRQLPALTDAFQASGFTWRGIIAWNKGRGSRTPHTGYFR
                     HQCEYIVWGSKGHLDKSPSGPFDGCMTFPVIPSKKMHPTGKPEELMAELVRTVNSGGT
                     VLDPFMGSGTTGVAALKAGRKFIGIETSDHYFEVAAQRLRGTITTTISAT"
     gene            complement(1528..1725)
                     /locus_tag="ACHURJ_003911"
     CDS             complement(1528..1725)
                     /locus_tag="ACHURJ_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Com family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR1282656.1"
                     /translation="MTQNVRCKNCNKLLARASFHYIEIKCPRCKTLNQITRAIEHPTH
                     MRSYDRGDRNPAPTSTYTGTH"
     gene            complement(1978..2118)
                     /locus_tag="ACHURJ_003912"
     CDS             complement(1978..2118)
                     /locus_tag="ACHURJ_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR1282657.1"
                     /translation="MSQKSLTAITFCVTAILIIWMLHGSLCEIRMNFWGAEFAAFLQC
                     KQ"
     gene            complement(2307..2567)
                     /locus_tag="ACHURJ_003913"
     CDS             complement(2307..2567)
                     /locus_tag="ACHURJ_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFR1282658.1"
                     /translation="MFRCPLCGASARIRTSRPENDSNTVRQKYYQCNNLECGVCFSTL
                     EAFHKFTSKHASGVHSSEGIPWHELPASHRGNNQMSLPLPQN"
     gene            complement(2610..3719)
                     /locus_tag="ACHURJ_003914"
     CDS             complement(2610..3719)
                     /locus_tag="ACHURJ_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HFR1282659.1"
                     /translation="MTDTTMQLLSQGTDPVKMPDFDILAEGKTLSGVAERLMSLSLTD
                     NRGFEADQLTITLDDADGQLQLPPRGARLTVLIGWKGEPLTEKGTYIVDEIAHEGPPD
                     RLTVSARSADFRDEFNVKREVSWHDVTIERVVSAIAHRYGLKPQISEMLMDIEIDHAD
                     QTEESDMSFLTRMAEMLGAITTVKSGNLLFIMPGSGVNAQGQPLPSFAITRSSGDRHQ
                     FRIADREAYTGIRAYWLDLNYGKKKKVSVKRRKPPKPKKEKSSSREGDYMEGAEGNVF
                     VLRKTYQNEQAARRAAAAKWQQLQRGAASFSITLVRGRAELYPEMHGTVKGFKSDIDN
                     QDWIIAKAEHTIDNSGFTTQLELEAKIPEWIAETE"
BASE COUNT          874 a          998 c          967 g         1075 t
ORIGIN      
        1 aaaaagagcc agtcagttgc ttaactgact ggcattacag cccttgagct gccgtttttt
       61 tattctgtta gttgtgaaac tgaagcgatt tagtcactat cgatctcatc aaatatggct
      121 cgctttgaga tattcctcaa gtaaaaaaac acctcttcct gcgatttctc acaaaaaaga
      181 ttcgttgaca aaaagtgaca aaattatgag attcccatca cacattttga catcaggaac
      241 ggtatgctga attcaccaag acgggaagac aagaggtaaa atttatgaca atgaacatta
      301 ccagcaaaca aatggaaatt actccggcca tccgccaaca tgtcgcagac cgtctcgcca
      361 aactggaaaa atggcaaaca catctgatta atccacatat cattctgtcc aaagagccac
      421 aagggtttgt tgctgacgcc acaatcaata cacctaacgg cgttctggtt gccagtggta
      481 aacatgaaga tatgtacact gcaattaacg aattgatcaa caagctggaa cggcagctca
      541 ataaactgca gcacaaaggc gaagcacgtc gtgccgcaac atcggtgaaa gacgccaact
      601 tcgtcgaaga agttgaagaa gagtagtcct ttatattgag tgtatcgcca acgcgccttc
      661 gggcgcgttt tttgtaaagc taacaaattg tttctattag cttatttctt ataaacatcc
      721 acaaactgtc cacatcagta atgatataca agcccgcaaa aaatgcgggc tttgtcttat
      781 gtagccgaaa tggttgtggt aatagtcccc cgaagcctct gcgctgctac ctcaaaataa
      841 tgatcactgg tttctatacc aataaatttt cgccctgctt tcagtgcagc cacacccgtt
      901 gtccctgacc ccataaaagg atcgaggact gttccgccac tattcacagt cctgaccagt
      961 tccgccatta gttcttctgg ttttccggtc gggtgcattt tctttgacgg gataaccggg
     1021 aacgtcatgc agccgtcgaa cggtcccgaa ggcgatttat ctaaatggcc tttgctgccc
     1081 cagacgatgt attcgcactg atgccggaaa tacccggtat gtggtgttcg tgaccccctc
     1141 cccttgttcc acgcaatgat gccgcgccat gtaaagccac tggcctgaaa cgcatcggtt
     1201 aaagctggta gttgtcgcca gtcagtaaat accagggcat aaccgcccgg cctgactgcg
     1261 cggtgtgcct gtgccatcca taattgtgtc cagtaagccc acgaacgggc gtccatgttt
     1321 tcacccgcga acccatcaaa gcggtgaagg ttctcgccgt tcagatattt tgcatggctg
     1381 ccctggttgg tgcgtccggc cttgtgtgtc gcacccgaac tgtaaggcgg gtcagtgatt
     1441 aaggcatcaa tgctttctgg ctcaatcagc ggcaatattt ccagcgcatt cccacgacag
     1501 agcgtgacat tgtcagtctg ataaacctta gtgtgttccg gtgtatgtgc tggttggtgc
     1561 tggattgcga tccccacggt cataactcct catgtgtgtg gggtgctcga tggctctcgt
     1621 tatctggtta agtgttttgc agcgcggaca ttttatttca atgtagtgaa atgaggcgcg
     1681 ggcaagtagt ttgttgcaat ttttgcatcg tacattttgc gtcatttgcg gcacctcttg
     1741 tgtggttact gctgccgata tgataacaaa tcgatcgttt ttatcgatcg gatttattgc
     1801 atcgatctgt tatgcctatt gcttatgtat ggtgccctca tttgtgaggg tagcaaaaag
     1861 aaaacccgca gtttttacgc tgcgggtttg ttgttcatgt ctgtgagata gggtgcctta
     1921 tcgaccttac cctggcaacc gattgacggg ggaatctctt cccccgtcgc ggtttcctta
     1981 ctgtttacac tgtaagaacg ccgcaaactc cgctccccag aagttcatcc gtatttcaca
     2041 cagcgagccg tgcagcatcc agatgatgag gattgccgtc acacagaacg tgatggctgt
     2101 aagcgatttt tgcgacatag cgcttgctcc tttttcgggg aggcgctaac ctatcacttg
     2161 ctaggtagac ggttagggcc tcggttaaac agagatgttt tccggggcct ttccacatct
     2221 ggccttcggg cattccctcc gaccatcagc cgaaaggcac ccgcgcgtaa tctatcgctt
     2281 ttttgttact ccggcaattc tgcctgttaa ttctgaggta aaggcaaact catctgattg
     2341 tttcccctgt gtgaagctgg cagctcatgc cacggaatac cttctgatga gtgaacgccg
     2401 gaggcgtgtt tcgatgtgaa tttatggaag gcttccagtg ttgagaagca tacgccgcat
     2461 tccaggttgt tacactggta atacttttgc cgcacggtgt ttgaatcatt ttccggacga
     2521 ctggtgcgga tacgggcaga tgcgccacaa agcggacaac ggaacatagc gacctccctt
     2581 aacgtggtgc tgccgctatt ctaagttctt cactctgttt ccgctatcca ttccgggatt
     2641 tttgcctcaa gctcaagctg tgtggtaaag ccgctgttat caatggtgtg ctcagctttt
     2701 gcaataatcc agtcctgatt gtcgatgtca cttttgaagc ctttcaccgt gccatgcatt
     2761 tcgggataga gttctgcgcg tccacgcacc agcgtgatgg agaatgatgc ggctccgcgt
     2821 tgtagctgct gccactttgc cgccgctgcg cgtcttgctg cctgctcgtt ctgataagtc
     2881 ttgcgtaaca caaacacatt gccttccgcg ccttccatat aatcaccttc acggctgctg
     2941 cttttctcct ttttgggttt tggcggtttg cggcgtttca cgctgacttt tttctttttc
     3001 ccgtaattaa gatcaagcca gtaagcgcgt atccccgtat acgcctcgcg gtcagcaatg
     3061 cggaactgat ggcgatcgcc gctgctgcgt gtgatggcga acgagggcaa cggctggccc
     3121 tgtgcgttca cgccactgcc gggcatgatg aataacagat taccgctttt taccgtggtg
     3181 attgcgccca gcatttccgc catgcgcgta aggaaggaca tgtcgctttc ttcagtctgg
     3241 tcggcgtggt cgatttcgat atccatcagc atttcgctga tttgcggttt cagaccatac
     3301 cgatgagcga tggcggatac cacacgctca atggttacat catgccagga cacctcacgt
     3361 ttaacgttaa attcatcccg aaaatctgcg cttctggctg aaacagtcag cctgtccggc
     3421 ggtccttcgt gagcgatttc atcaacaatg taagtgcctt tttctgtcag cggttctcct
     3481 ttccagccaa tgagaaccgt caggcgcgcg ccccgtggcg gtagctgcaa ctgaccatcc
     3541 gcatcatcca gcgtgatggt gagctggtcc gcctcaaatc ctcggttgtc ggtcagtgac
     3601 aggctcatca ggcgctcggc cacgcctgac agcgttttac cctccgcgag aatatcaaaa
     3661 tccggcattt tcacggggtc tgtgccctga ctgagcaatt gcatggtggt atcggtcatc
     3721 tgttccctcc ctgtgcggca tggtcgcatg tgcgtgcgga gggggttact gctttttgtt
     3781 gtcgccgtgg cgggagaacg gcgcaggggt gagattacgc gcgtggtggg tgatgattgt
     3841 tgccgaatca tttaacggat acaaggggct gaagctatga gtgaaactcg ttttcatggt
     3901 gcccgtgtta cgga
//
LOCUS       DAZPEB010000256         3911 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZPEB010000256 DAZPEB010000000
VERSION     DAZPEB010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3911
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.261"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(313..732)
                     /locus_tag="ACHURJ_003915"
     CDS             complement(313..732)
                     /locus_tag="ACHURJ_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1282660.1"
                     /translation="MVHEDKARKEFASRLALACENAGYEQHGRQAEIARRMKLTPKAV
                     SKWFNGETIPRREKLRELATLIGTTPTYLLGEDTEESGQVRFYQELNPRQKIIIDLLD
                     ELPDSETDELLKTLEEKKQKYNAIYEELARKKKQKAS"
     gene            812..1066
                     /locus_tag="ACHURJ_003916"
     CDS             812..1066
                     /locus_tag="ACHURJ_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR1282661.1"
                     /translation="MNQKTLEDVIKTVRVAVVADVCGVSQRAIYKWMDNGKLPRTEYT
                     GETNYAEKIALASNGLFSADAILTIGRNKTTTKKLMGVDS"
     gene            1063..1488
                     /locus_tag="ACHURJ_003917"
     CDS             1063..1488
                     /locus_tag="ACHURJ_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFR1282662.1"
                     /translation="MKIKHEHIRMAMNAWAHPDGEKVPAAKITKAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWVEKDTPDALKKIQALLPAIEKAMPPLLVARMRSHSSAYFRE
                     LVETRERLVRDADDFVAVAIAGFNQMNRGGPAGNIVAVH"
     gene            1560..2630
                     /locus_tag="ACHURJ_003918"
     CDS             1560..2630
                     /locus_tag="ACHURJ_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HFR1282663.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSILQTMQGRVLDGDLITGWEKRQVLKEDNGN
                     ISQTAKSPAERKRAQRERERKREQNGDCHGASRYVTHMSRRVTTDKDTDKDTDQEDQN
                     TMVHGVKNATNQAGDVQTVNPGQPAGTTPEADSAYALKADSGAVQQAMTASPEQSHQL
                     QQPEADSAIQREADRVVPENTGQSVGRVDYPDVFEQVWREYPLRAGANPKKSAFSAWK
                     ARLREGVPPEAMLDGVRRYARYLAATGKTGTEFVQRATTFFGPDRNFENPWLLPVSGT
                     NNQRCVNHISEPDNEIPPGFRG"
     gene            2671..3093
                     /locus_tag="ACHURJ_003919"
     CDS             2671..3093
                     /locus_tag="ACHURJ_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR1282664.1"
                     /translation="MAKVFTQEEREKIKGQVLELVRQSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPSAVDPDLIWSLPDG
                     EIRRYDSRLNIICRECRKSEVMQRILSFYQGNFQEVMA"
     gene            3090..3395
                     /locus_tag="ACHURJ_003920"
     CDS             3090..3395
                     /locus_tag="ACHURJ_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFR1282665.1"
                     /translation="MRVRVYIAGPMTGYKNFNREAFHNAEEELKREGHTVLNPAVLPD
                     GLTQPQYMDICMAMIRCVDAIYMLKGWQRSAGAKAELALAEKLGHAVIFQETASVQD"
     gene            3382..3837
                     /locus_tag="ACHURJ_003921"
     CDS             3382..3837
                     /locus_tag="ACHURJ_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFR1282666.1"
                     /translation="MCKTNYQALRERYSPIQVPECPVCGDEMSIQRIFSRTHIVYACT
                     GEGDDGYFKTGRTFADEHYLKSRVTVVDVSAPDVLALLKELEVKDKRIAELTDALTQM
                     INAHKTTIRFGHERITECGGDCDSPEKMISENPDIRMAEAVLRAGIKTE"
BASE COUNT         1071 a          842 c         1049 g          949 t
ORIGIN      
        1 atcgatagta tccatgctga cctcatttcc ccttaacgcc ggggtagcgg aacaaaaacc
       61 tgctgcatag ttattaaagt tgaaccctgc cgtcatgttc ttacgcctcg ggctggctac
      121 ttaacccctg accactgcct ggtaactcga agtattgccc tgcattctgt ggggcggggt
      181 gggtggcagg tatataatgt actttgcgtt cattgttgta aagtgcttat ggtacatttt
      241 gtgtgtaaaa aaatgagatg ggataaagtg aagcacaaac ccggaggaag gcgctaccgg
      301 atttatgctg gtttaagagg ctttttgttt tttctttcgt gctaactctt cgtaaattgc
      361 attgtacttc tgttttttct cttcaagagt ttttaaaagt tcatctgtct cactgtcagg
      421 gagctcgtcc agaaggtcaa tgatgatttt ttgtcttgga tttaactcct gatagaaacg
      481 tacctgtcca ctttcttctg tatcctctcc caaaagatag gttggtgttg ttcctattag
      541 tgttgctaat tcccttaatt tctcccggcg aggaattgtt tcgccattaa accatttgct
      601 aaccgctttt ggtgttaatt tcattcgacg ggcaatttct gcctgccttc catgttgttc
      661 ataaccagcg ttttcacagg ctagcgcaag cctactggcg aactctttac gcgctttatc
      721 ttcatgaacc ataagttcaa tgatattcgc tcttgaatgt actgtcagtt ctgttatagc
      781 atgtactcaa agttcacatt gtgagggtga tatgaaccag aaaacacttg aagatgtaat
      841 caaaactgtt cgcgttgctg ttgtggccga cgtttgtggt gtcagccaaa gagcaatcta
      901 taaatggatg gataacggaa aattgcctcg cacagaatat accggcgaaa caaattacgc
      961 tgaaaaaatc gctcttgcat caaacggatt attttctgcc gatgcaattt taactattgg
     1021 caggaataaa actactacga aaaagctgat gggagttgat tcatgaaaat caagcatgaa
     1081 cacatccgca tggcgatgaa tgcctgggcg catccggacg gcgaaaaagt accggctgcg
     1141 aaaattacca aagcgtattt cgagctggga atgacgttcc cggaactgta tgacgacagc
     1201 catccggaag ccctggctcg caatactcag aaaattttcc gctgggtgga gaaagacacc
     1261 cctgatgcgc ttaaaaaaat tcaggcgttg ttaccagcta tcgaaaaagc aatgccacct
     1321 ctgctggtgg cccgaatgcg cagccatagc tcagcctatt ttcgggaact agtggagacg
     1381 cgggaacgac tggtgagaga cgctgatgat tttgtcgcag tggcgatcgc tggtttcaac
     1441 cagatgaatc gtggtggccc tgcaggaaat attgtggctg tgcattgact cgcaatattc
     1501 ataccggatc acttccggca atttgtgagt aaaaagattc ggtatcaaaa gaggtgagta
     1561 tggctaacgc ctggctcaga ttatggcatg acatgccaaa tgaccctaag tggcgaacaa
     1621 ttgccagggt gtcagggcag ccaattgcaa cagtgatggc agtgtatatc cacctcctgg
     1681 tgagcgcgtc acgaaatgtc acgcgaggtc acattgatgt cacgacagaa gatttggcaa
     1741 gtgcgctcga cgtgacagaa gaggtaattg attcaatttt gcagacgatg caggggcggg
     1801 tacttgatgg tgatttaatc actggatggg aaaaacgcca ggtgctgaaa gaggacaacg
     1861 gcaatatttc gcaaaccgca aaatctcctg cagagcgcaa gagggcgcag cgagagaggg
     1921 aaagaaagcg ggaacaaaat ggcgattgtc acggcgcgtc acgatatgtc acgcacatgt
     1981 cacgacgagt cacgacagat aaagatacag ataaagatac agatcaagaa gatcaaaaca
     2041 ctatggtcca tggcgtaaaa aacgccacga accaggcagg ggatgttcag accgtcaatc
     2101 ctggtcagcc agcaggcacg acaccggaag ccgattcagc gtatgcgctg aaagccgatt
     2161 cgggcgctgt gcagcaggcg atgaccgcaa gtccggagca atcacaccaa ctgcagcagc
     2221 ctgaagccga ttccgccatt cagcgggaag ccgatcgggt agtcccggaa aacaccgggc
     2281 agtctgtggg acgagtggat tatccggatg tgttcgaaca ggtctggcgg gagtacccgt
     2341 tgcgtgccgg ggcaaacccg aagaaatccg ctttcagtgc ctggaaggcc agattacgcg
     2401 agggggtgcc accagaggcc atgctggatg gcgtgaggcg ttacgcaaga tacctggcgg
     2461 ctaccgggaa aacgggaacg gaatttgttc agcgagcgac gacgtttttt ggaccggacc
     2521 ggaattttga gaacccctgg ttgctcccgg taagcggcac gaacaaccag cgttgtgtga
     2581 atcatatttc tgaaccggat aacgaaattc cgccgggctt cagggggtaa gtgttaattt
     2641 ctggtcatga ggtaattttc aggagggctt gtggcaaaag tttttacaca agaagagcgg
     2701 gaaaaaatta aagggcaggt tcttgaactc gtacgccaga gtgggcgcga gacgttacga
     2761 caactggaag ctaaaactgg ggcaacaaga tatctgatga gcgttctggc cagagagctg
     2821 gttgccagtg gcgatgtata caactctggt tacgggttat tcccgtctga acaggcgcgt
     2881 aaggactggc aaaatgcccg taaaaagctc tcaagggcaa agctgaagaa accatctgcg
     2941 gttgatccgg accttatctg gtcattacct gacggagaaa tacgtcgcta cgacagtcgc
     3001 ctaaacataa tctgtcgcga gtgccggaag agcgaagtta tgcagcgcat attgtcgttt
     3061 tatcagggta attttcagga ggtgatggcg tgagggtgag agtctatatc gccggtccaa
     3121 tgaccgggta taaaaatttc aaccgtgagg cgtttcacaa tgcggaagag gaactgaaac
     3181 gggaagggca taccgtctta aacccggcag tacttccgga cgggctgaca cagccgcagt
     3241 acatggatat ctgcatggcg atgattcgtt gtgtggatgc gatttacatg ctgaaaggct
     3301 ggcagcggtc agcaggcgct aaggcagaac tggcgctggc ggagaaactg gggcatgcgg
     3361 tgatttttca ggagacagcc agtgtgcaag actaattacc aggcattacg tgaacgttat
     3421 tcaccaattc aggtgccgga atgtcctgtt tgtggcgatg aaatgtcgat acagcgcata
     3481 ttttccagaa cgcatattgt gtatgcctgc acaggtgagg gggatgatgg atattttaaa
     3541 actggtcgaa cttttgcgga tgagcattac ctgaaatcgc gcgtaaccgt cgttgatgtt
     3601 agcgctcctg acgtactggc tttactgaaa gagctggaag ttaaagacaa acgcattgca
     3661 gaattgacag atgcgcttac acaaatgatt aatgcgcaca aaaccacaat tcgttttggt
     3721 catgaacgca taactgaatg tggtggtgat tgcgactcgc cggaaaagat gatttcagaa
     3781 aatccggata tcagaatggc agaggctgtt ttgagagcag gaataaaaac tgaataatta
     3841 aatttagcac agcaaataaa atttaatcct taaccggagg gatttctgca ccctcagaac
     3901 atcaggaggc c
//
LOCUS       DAZPEB010000257         3908 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZPEB010000257 DAZPEB010000000
VERSION     DAZPEB010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.262"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(382..1557)
                     /gene="senB"
                     /locus_tag="ACHURJ_003922"
     CDS             complement(382..1557)
                     /gene="senB"
                     /locus_tag="ACHURJ_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin production-related protein TieB"
                     /protein_id="HFR1282667.1"
                     /translation="MNIFTLSKAPLYLLISLFLPTMAMAIDPPERELSRFALKTNYLQ
                     SPDEGVYELAFDNASKKVFAAVTDRVNREANKGYLYSFNSDSLKVENKYTMPYRAFSL
                     AINQDKHQLYIGHTQSASLRISMFDTPTGKLVRTSDRLSFKAANAADSRFEHLRHMVY
                     SQDSDTLFVSYSNMLKTAEGMKPLHKLLMLDGTTLALKGEVKDAYKGTAYGLTMDEKT
                     QKIYVGGRDYINEIDAKNQTLLRTIPLKDPRPQITSVQNLAVDSASDRAFVVVFDHDD
                     RSGTKDGLYIFDLRDGKQLGYVHTGAGANAVKYNPKYNELYVTNFTSGTISVVDATKY
                     SITREFNMPVYPNQMVLSDDMDTLYIGIKEGFNRDWDPDVFVEGAKERILSIDLKKS"
     gene            complement(1626..3887)
                     /locus_tag="ACHURJ_003923"
     CDS             complement(1626..3887)
                     /locus_tag="ACHURJ_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFR1282668.1"
                     /translation="MNVIKLAIGSGILLLSCGAYSQSISEKTNSDKKGAAEFSPLSVS
                     VGKTTSEQEALEKTGATSSRTTDKNLQSLDATVRSMPGTYTQIDPGQGAISVNIRGMS
                     GFGRVNTMVDGITQSFYGTSTSGTTAHGSTNNMAGVLIDPNLLVAVDVTRGDSSGSEG
                     INALAGSANMRTIGVDDVIFNGNTYGLRSRFSVGSNGLGRSGMIALGGKSDAFTDTGS
                     IGVMAAVSGSSVYSNFSNGSGINSKEFGYDKYMKQNPKSQLYKMDIRPDEFNSFELSA
                     RTYENKFTRRDITSDDYYIKYHYTPFSELIDFNVTASTSRGNQKYRDGSLYTFYKTSA
                     QNRSDALDINNTSRFTVADNELEFMLGSKLMRTRYDRTIHSAAGDPKANQESIENNPF
                     APSGQQDISALYTGLKVTRGIWEADFNLNYTRNRITGYKPACDSRVICVPQGSYDIDD
                     KEGGFNPSVQLSAQVTPWLQPFIGYSKSMRAPNIQEMFFSNSGGASMNPFLKPERAET
                     WQAGFNIDTRDLLVEQDALRFKALAYRSRIQNYIYSESYLVCSGGRKCSMAEVIGNDW
                     EGISDEYSDNMYIYVNSASDVIAKGFELEMDYDAGFAFGRLSFSQQQTDQPTSIASTY
                     FGAGDMTELPRKYMTLDTGVRFFDNALTLGTIIKYTGKARRLSPDFEQDEHTGAIIKQ
                     DLPQIPTIIDLYGTYEYNRNLTLKLSVQNLMNRDYSEALNKLNMMPGLGDESHPANSA
                     RGRTWIFGGDIRF"
BASE COUNT          974 a          922 c          920 g         1092 t
ORIGIN      
        1 ccggggtgat gcaggataag tgcgcccggg aatcggggta agtataaaaa acggccgatt
       61 acagtattaa aaccgtccac agaaagaaaa aaacaccggc gtgatgtgaa aaacagctta
      121 ccgcacgagg cataagacgc ggacagtggt atcgcttagt ttttcaattt cctatacgtg
      181 aacaggcata gctgtgattt cattgaatta ttggcacaaa tcagttgatc gtacaatgct
      241 gtgcctgtct cgttttctgg agattcctca gcctcatgga gcggcgtaac aggcgtaccg
      301 gcaggacaga gatgtttgct tttcctagtc cggccagttt agtggaataa cggagttttt
      361 atcccatgtt ttttcccccg atcacgactt tttcaaatca atgctcagaa tacgttcttt
      421 agctccttcc acaaacacat caggatccca atcgcggtta aagccttctt tgatgccaat
      481 gtaaagggta tccatatcgt ccgacaacac catctggttt gggtagaccg gcatgttaaa
      541 ttcacgggtg atgctgtatt tggtggcatc cactacgctg atggtgccgc tagtgaagtt
      601 ggtgacatac agttcgttat atttcggatt gtatttcacc gcgttagctc cggctcctgt
      661 gtgcacatag ccaagctgtt taccgtcgcg taagtcaaaa atatagaggc catcttttgt
      721 accggaacga tcgtcatggt cgaataccac cacaaaggca cggtcagaag cggagtccac
      781 cgccagattc tgcacacttg tgatttgtgg tctcggatct ttcaacggga tggtacgcag
      841 cagcgtctga tttttcgcat caatttcgtt gatgtaatct cttccgccaa cgtagatttt
      901 ctgtgttttt tcatccatcg tcagaccata cgctgtacct ttgtaagcat ccttaacctc
      961 gccttttaag gcaagcgtcg tcccgtcgag cattaacagc ttatgcagag gcttcatgcc
     1021 ctcggccgtt ttcagcatat tgctataact cacaaacagg gtatcggaat cctggctgta
     1081 aaccatatgg cgcaaatgct caaaacgcga atctgcagcg tttgccgctt taaaacttaa
     1141 cctgtcgctg gttcttacca gtttaccggt tggggtgtca aacatactga tacgcaggga
     1201 cgctgactgg gtgtgtccga tatagagctg atgtttatcc tgatttatcg ccagcgaaaa
     1261 tgcccggtat ggcatcgtgt atttattttc gactttcagc gaatctgaat taaacgaata
     1321 cagatagcct ttattggctt cacgatttac acgatcggtg actgctgcaa acaccttttt
     1381 actggcatta tcaaacgcca gttcatagac gccttcatca ggggactgaa ggtaattcgt
     1441 tttcagggca aatcgcgaaa gttcgcgttc aggtggatcg atagccatgg ccatcgtggg
     1501 taaaaatagt gaaattaaca agtatagcgg tgctttggat aaagtgaaaa tattcatttt
     1561 tcgttcctgc aataaaattg acataagaga gaaaacgtat tgtctgaacc tggcggaaat
     1621 tgtcatcaga aacgaatgtc cccgccaaat atccatgttc tgccacgcgc ggaattggct
     1681 gggtggctct cgtcaccaag acctggcatc atgttgagct tattcagcgc ctccgaataa
     1741 tctctgttca tcaggttttg taccgaaagt ttcagtgtca ggttgcggtt gtactcgtaa
     1801 gtaccataga gatcgataat cgttgggatc tgcggcaaat cctgtttgat tattgcgccg
     1861 gtatgttcgt cctgctcaaa atcaggcgac aggcgacgag ccttgcctgt gtattttatg
     1921 atagtgccca gggtcaacgc gttatcgaag aagcgaacac cagtatccag cgtcatgtat
     1981 tttctgggca gttcggtcat atcccctgcg ccaaagtagg tgctggcgat ggaggttggc
     2041 tggtctgttt gctgctggct gaaagagagt cggccaaaag caaaacctgc atcataatcc
     2101 atctccagtt cgaagccctt tgcaataacg tcgcttgccg agttaacgta gatgtacata
     2161 ttgtcgctgt attcatcgct aatgccctcc cagtcattgc caatcacctc agccatactg
     2221 catttacgac ctccagaaca aaccagataa gactcgctgt agatgtagtt ctggatcctg
     2281 ctgcggtacg ccagagcctt aaagcgcagg gcatcctgtt cgaccagtaa atctctggta
     2341 tcaatgttaa aacccgcctg ccaggtttct gcacgttcag gcttcaggaa tgggttcatg
     2401 gatgcgcctc ctgaattaga gaagaacatc tcctggatgt tcggggcgcg catggatttg
     2461 ctgtagccaa tgaacggctg aagccatggt gttacctgag cagaaagctg aactgaaggg
     2521 ttgaagccac cctctttatc gtcaatatcg tagctaccct gtggcacgca gataacgcgt
     2581 gaatcgcagg cgggcttgta ccctgtgatc ctgttacgtg tgtagttgag attgaaatct
     2641 gcctcccaga tgccgcgcgt aaccttcagc ccggtataca gcgctgaaat atcctgctgg
     2701 ccggagggtg cgaacggatt gttctcgatc gattcctgat tcgctttcgg gtcgcccgcc
     2761 gctgagtgaa tggtccggtc atagcgggta cgcatcagtt tgctgcccag cataaactcc
     2821 agttcattgt ccgcgacagt gaaccggctg gtattgttga tatccagcgc gtcagaacga
     2881 ttttgcgctg aggttttgta gaaagtatac agcgagccat cacgatactt ttgattaccg
     2941 cgactggtac tggccgttac gttaaagtca attaattcag aaaaaggggt gtaatgatat
     3001 ttaatgtaat agtcgtcact ggttatatca cgacgtgtaa atttattttc ataggttcga
     3061 gcggaaagtt cgaagctgtt aaattcgtct ggtctgatat ccattttata cagttgggat
     3121 ttggggttct gcttcatata tttatcataa ccaaactctt tgctgttaat tccagaacca
     3181 tttgagaaat tagagtacac agaactgcca ctcacagcag ccataacgcc aatgcttccc
     3241 gtatccgtaa aagcgtcgct ttttccacca agggcgatca ttccgctgcg tcccagccca
     3301 ttactaccga cagagaaacg tgaacgaagg ccatatgtat taccgttaaa tattacatcg
     3361 tcaacgccaa tagtacgcat atttgcacta ccggcaaggg cgttgatccc ttcagagcca
     3421 ctgctgtcac cgcgtgtaac atcaactgct accagtaagt taggatctat aagtacgcca
     3481 gccatattgt tagttgaacc atgcgccgtt gttccggagg tagaggttcc gtaaaaactc
     3541 tgggtaatac catcgaccat agtgtttaca cgaccaaatc cgctcatgcc tcgaatattc
     3601 acactgattg ctccctgacc aggatctatt tgagtataag taccaggcat actacgcact
     3661 gttgcgtcaa gtgattgcag gtttttgtcc gttgtccggg aactggtcgc gcctgttttc
     3721 tcgagagctt cctgctcact ggtcgtcttc ccgacagaaa cgctgagcgg actgaattct
     3781 gccgctcctt ttttgtcgga attagttttt tcactgatgg attgtgagta agcaccgcag
     3841 ctgagcaata atatgcctga gccgatagcc agttttataa cgttcataaa tttccttgga
     3901 tataatat
//
LOCUS       DAZPEB010000258         3853 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZPEB010000258 DAZPEB010000000
VERSION     DAZPEB010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.263"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..409
                     /locus_tag="ACHURJ_003924"
     CDS             <1..409
                     /locus_tag="ACHURJ_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFR1282669.1"
                     /translation="FTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVR
                     ISIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIA
                     EGVESEDVARELIKLGCVQAQGYLYQKPMPFSA"
     gene            447..1775
                     /locus_tag="ACHURJ_003925"
     CDS             447..1775
                     /locus_tag="ACHURJ_003925"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFR1282670.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGSYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKIYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(1822..3147)
                     /gene="rimO"
                     /locus_tag="ACHURJ_003926"
     CDS             complement(1822..3147)
                     /gene="rimO"
                     /locus_tag="ACHURJ_003926"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFR1282671.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPICEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            3360..3743
                     /gene="bssR"
                     /locus_tag="ACHURJ_003927"
     CDS             3360..3743
                     /gene="bssR"
                     /locus_tag="ACHURJ_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFR1282672.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
BASE COUNT         1008 a          856 c          949 g         1040 t
ORIGIN      
        1 ttttaccacc atcatgaaag aaaatggcct gaaggcgaac agcctctgtg ttgagatcac
       61 tgaaaccgtg atcgagcgaa ttaatgaaca tttttatctc aatattgaac aactgcgtaa
      121 acaaggggta cggatatcga ttgatgactt tggcaccggt ttgtcaaacc tgaaacgttt
      181 ttatgaaatt aatccagaca gcataaaggt ggactcgcaa tttaccggcg atattttcgg
      241 tactgcggga aaaattgtgc gcattatttt cgatctggca cgctataacc ggatcccggt
      301 gattgcggaa ggcgtagaga gcgaagacgt tgcacgcgaa ttaattaaat taggatgtgt
      361 tcaggctcag gggtatctgt accagaaacc tatgccattc tccgcctagg ataaaagtgg
      421 aaaattagta aaagagtagt ttacgtatgt ccagaatcaa taagttcgta cttacagtca
      481 gtctgctgat ttttatcatg atttcagcag ttgcctgcgg gatctacact caaatggtaa
      541 aggaacgggt gtatagcctg aaacagtccg ttattgatac tgcttttgcg gtggcaaata
      601 ttgctgaata tcggcgtagc gtggcaattg atcttatcaa cacgctaaat cccactgagg
      661 aacagctgtt ggttggtttg cgcacagctt acgccgactc ggtttccccc tcttatttgt
      721 acgatgtcgg tccttatctg atttccagtg acgaatgtat tcaggtaaag gagttcgaga
      781 aaaattattg tgcagatatt atgcaggttg tgaagtatcg acatgtcaaa aatacagggt
      841 ttatctcttt tgacggtaaa accttcgtct attacctcta tccggtaact cacaatcgta
      901 gtctgatatt tttgcttggt ctggagcgtt tttctttact gtcaaaatcg ctggcgatgg
      961 acagcgagaa cctgatgttc tctctattta agaacggtaa accggtgacc ggtgatgaat
     1021 ataatgctaa aaacgccatc ttcaccgttt cggaagcgat ggagcacttc gcctatttgc
     1081 cgaccggatt gtacgtattt gcgtataaaa aagatgttta tttgcgggtc tgtacattga
     1141 ttattttctt tgccgcattg gtggctgtga tatcgggtgc cagttgcctc tatctggtac
     1201 gcagagtgat taatcgtggt attgtggaga aagaagccat cattaataac cattttgaac
     1261 gcgtactgga tggcgggctt ttcttttcgg ctgccgatgt caaaaaactc tacagtatgt
     1321 ataactcggc gttcctggac gacctgacca aagcaatggg cagaaaatcc tttgacgaag
     1381 atttaaaagc gctgccggaa aaaggcagtt atttgtgcct gtttgacgtc gataaattca
     1441 aaaacattaa cgacaccttc ggtcatttgc tgggcgatga agtgttgatg aaagtggtga
     1501 aaatccttaa atcacagatc ccggtagata aaggtaaaat ctaccgcttc ggcggtgacg
     1561 aatttgcggt gatttatacg ggtggaacgc tggaagagtt gctatcgatt ctaaaagaaa
     1621 tcgttcattt ccaggtggga agcattaatt taagtaccag tatcggtgta gcgcattcaa
     1681 atgaatgtac caccgtcgaa cgcttgaaaa tgctggcgga tgagcggctg tataagagta
     1741 agaaaaacgg cagggctcag attagctggc agtaatcatt attcgcaggc cggacaaatg
     1801 attttgcccg gcctgaatta attaaacccg gctaccccac aaatcgtact catcggcgtg
     1861 ctcgactttc acacgcagga tatcacccgg cttaacgttg gtttcaccat tgagataaac
     1921 cgcgccgtcg atttccggtg catctgccat gctgcgacca atcgcgcctt cttcgtccac
     1981 ttcgtcgata atcaccagaa tttcacggcc cactttctct tgcaggcgct cggcggaaat
     2041 ctgctgctgc agctgcatga aacggttcca gcgttcttct ttaacttctt ccggaacctg
     2101 gtcaggcagg gcattggcgt ctgcaccttc aaccgggctg tatttaaagc agccaacgcg
     2161 atccagacgc gcttctttca ggaagtcgag tagcatctgg aaatcttctt ctgtctcgcc
     2221 agggaagccg acaataaagg ttgagcgtag ggtcagttcc gggcagattt cgcgccactg
     2281 tttgatgcgc gccagttggc gatctacaga acccggacgc ttcatcagtt tgagaatgcg
     2341 cgggctggcg tgctgcaacg gaatgtccag atacggcagg attttgcctt ctgccatcag
     2401 tgggatgacg tcgtccacat gcggataagg gtaaacgtag tgcagacgtg tccagatccc
     2461 cagtttcgat aactgttcgc acaggctgac catgctggtt tttaccggct cgccgttgtg
     2521 gaagccagta cgatgtttaa catcaacgcc ataggcggaa gtatcctgcg agatcaccag
     2581 aatctcttta acgcccgcat ctaccagacg tttcgcttca cttaacactt cgcaaatcgg
     2641 acggctcacc aggtcgccgc gcatagacgg aataatgcag aaggtgcagc ggtgattaca
     2701 gccttccgaa attttcagat aggcataatg acgcggcgtc agtttcacac cttgttctgg
     2761 caccaggctc aggaatgggt tgtgtttcgg ttttggcacg tagtgatgaa cgtgctccag
     2821 aacctgctca tagctatgag gcccggtgat ttccagcact ttcgggtgga cttcgcggat
     2881 ctgatcttct tttgccccca gacaaccggt cacaattacc ttgccgtttt cattcaacgc
     2941 ttcaccaatg gcttccagtg attcttgtac cgcgctgtca ataaagccgc aggtgttgac
     3001 gatcaccatg tccgcatcgt catagctcgg taccacgtca taaccttcag tgcggagttc
     3061 ggtgagaata cgctctgaat cgacaaggtt tttcggacag ccaagggaaa caaagccgat
     3121 tttcggctgg ggagttactt tgctcatagc ttaaaaaata ttcagttaca ggaaaggtca
     3181 gggcagggat tctacagagt tctggataaa atttgtatcg caatctcatt cgctggcgga
     3241 ggcgaaggaa atgtaaattt tgttaattcg gcgtgaagaa ttgatcctgg acagcatttt
     3301 gctcaaaaaa tagccatact atttaattgc aacaaggctg gaaagaggag gatcgaagta
     3361 tgttcgttga cagacagcga atcgatctgc tgaaccggtt gatcgacgca cgcgttgacc
     3421 tcgctgcata cgtgcaactg aggaaggcaa aaggatacat gtccgtcagc gaaagcaatc
     3481 atctacgaga taactttttt aaactgaatc gcgaactgca cgataaatcg ctgcggttga
     3541 atcttcatct ggatcaggaa gagtggagtg ctcttcatca tgctgaagaa gcattagcga
     3601 cagccgcagt atgtttgatg agtgggcacc atgattgccc gactgttatt accgtcaacg
     3661 ccgataagct tgaaaattgt ctgatgagct taacgctgag tatccagagc ctgcagaagc
     3721 acgccatgct tgagaaggcc tgaaaactaa gggggagaaa gcgtctcccc cttcatgttt
     3781 aagtttttgt aaaaatgaat ttgttatctc ctccactgac tacgctttaa gccagagtca
     3841 atccggaggc gtt
//
LOCUS       DAZPEB010000259         3822 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZPEB010000259 DAZPEB010000000
VERSION     DAZPEB010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.264"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            1..214
                     /locus_tag="ACHURJ_003928"
                     /pseudo
     CDS             1..214
                     /locus_tag="ACHURJ_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
     gene            196..1578
                     /gene="torZ"
                     /locus_tag="ACHURJ_003929"
                     /pseudo
     CDS             196..1578
                     /gene="torZ"
                     /locus_tag="ACHURJ_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
     gene            complement(1743..2714)
                     /gene="cmoB"
                     /locus_tag="ACHURJ_003930"
     CDS             complement(1743..2714)
                     /gene="cmoB"
                     /locus_tag="ACHURJ_003930"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFR1282673.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRVVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2711..3454)
                     /gene="cmoA"
                     /locus_tag="ACHURJ_003931"
     CDS             complement(2711..3454)
                     /gene="cmoA"
                     /locus_tag="ACHURJ_003931"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFR1282674.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MISMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            complement(3495..>3822)
                     /locus_tag="ACHURJ_003932"
     CDS             complement(3495..>3822)
                     /locus_tag="ACHURJ_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFR1282675.1"
                     /translation="LRLQYRVAYGDGGFSELQSAIRIHGNAVEYIPIAIVLMLFMEMN
                     GAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRSGMSATWCALLLMVLANLWYMPWE
                     LVFSLR"
BASE COUNT         1011 a          951 c         1010 g          850 t
ORIGIN      
        1 cataggcgag ctggctaccg tgaatcctgg taccgatctc accgtcgttg atgccagtgg
       61 caaacagccg atcgttctgt tgcagggtta tcaaatgcag ggcagtgaaa acacgctcta
      121 cctggcggca ggtcaacggc tggcgctagc cacattaagt gaagaaggta tcaaggcgct
      181 cacgctaaac ggggaatggc aggctgggga attcagcgcc agcaatatgg tgaacagaaa
      241 cactggatgc tggtgacgct ggcggcggtg ttagggcaga ttggtacacc gggcggcggt
      301 tttggtttct cttatcatta ctccaatggt ggcaacccga cacgtgttgg cggcgtgctg
      361 ccggaaatgt ctgcggcgat tgccggacag gccagcgagg ccgctgatga tggcggaatg
      421 agggcgattc ctgttgcccg tatcgtcgat gcgctggaaa atcctggcgg caaatatcag
      481 cataacggta aagagcaaac gtatccgaat atcaaaatga tctggtgggc gggtggcggt
      541 aactttaccc atcaccagga tactaatcgt ctgattaaag tatggcagaa accggagatg
      601 atcgtcgttt ctgaatgcta ctggaccgca gctgctaaac atgcagatat cgtattaccg
      661 atcaccacat cgtttgagcg caatgacttg acgatgaccg gtgattacag caaccagcat
      721 attgtgccga tgaagcaggc tgtcgctccg caatttgaag cgcgaaacga ttttgatgtg
      781 tttgccgatc ttgcggaatt actgaaacct ggcggaaaag agatctatac cgaaggtaaa
      841 gatgaaatgg cgtggctgaa atttttctat gatgccgctc agaaaggtgc ccgtgcgcaa
      901 cgcgtgacta tgccgatgtt taatgccttc tggcagcaaa ataaactgat cgaaatgcgc
      961 cgcagcgaga agaacgaaca gtacgttcgt tatgctgatt tccgcgccga tccggtgaaa
     1021 aatgcgctgg gtacgccaag cggcaaaatt gagatttact ccaaaacgct ggaaaaattt
     1081 ggctataagg attgcccggc acatccaacc tggcttgcgc ctgatgagtg gaagggtacc
     1141 gccgacgaga agcagttgca gcttctgacc gcacatccgg cacaccgttt acatagtcag
     1201 cttaactatg cggaactgcg taaaaaatat gcggttgcag atcgtgaacc aatcactatt
     1261 cataccgaag atgctgctcg ctttggtatt gcgaatggtg atctggtgcg tgtgtggaac
     1321 aaacgtggtc agattctgac aggcgcggtg gtgactgacg ggatcaaaaa aggcgtggta
     1381 tgcgtgcatg aaggtgcatg gccagatctg gaaaatggct tgtgtaaaaa cggcagtgcg
     1441 aacgtgttaa cggcggatat ccccagctcg cagctggcaa atgcctgtgc cggtaactct
     1501 gcgctggtgt atatcgaaaa atatacgggt aacgcaccga agttaacggc atttgataag
     1561 cctgctgttc aggcgtaaga agtgaatagc gcccggaaac gggcgctgag gttatgcaaa
     1621 cgtaaaactg cctgatgcgc tatgcttatc aggtctacat agtccctgca atagattgaa
     1681 ttagcaaggg tttgtaggcc ggataaggca ttcacgctgc atccggcagt aataccagac
     1741 ctttacggct tgcgcgcaat cagcaccgcg cgtttaggcg caggataacc ttcaactgtc
     1801 ttactcggat catgtgggtc gaggaaatcg gacagagatt cggtaaccat ccattcagtg
     1861 cgtcgctgct cttcagtagt ggtaacgcac acatccacaa cgcggatatc tacaaaacca
     1921 cacttcttca gccagttttt cagcgccagc gcggaaggga tgaaatagac attacgcatt
     1981 tgcgcgtaac gatcgcctgg taccagcacc gtgttctcat cgccatcaat aaccagcgtt
     2041 tccagcacca gttcaccctc attcaccagt tgatctttca actgccagag atgctccagc
     2101 ggcgaacggc gatggtaaag cacgcccatc gaaaagacgg tatcaaaggc tttcagtgcc
     2161 ggaagctgtt caatacctaa cggtaacaaa tgtgcgcgct gatcgttacc cagtagttta
     2221 cgcaccgctt caaactggca cagaaacagt tgcgtagggt cgatccccac cgcgaggtgc
     2281 gcgcctgcgc caatcatgcg ccacatgtga taaccgctgc cgcagccgac atccagaatg
     2341 gtgcgtccgg ttaaatcaga aagatggggc agaacacgat cccatttcca gtcggaacgc
     2401 cattcggtat cgatgttgac gccatacagt gagaacggcc ctttgcgcca tggcatcagg
     2461 ttccgcataa gcgtttcaat gcgtttaatt tgcccggtgc tcagtggctc ttcgctttcg
     2521 gcggttacgc tatgcaataa atccagacga tacggtttaa tttcaggcag aaattccacc
     2581 gcgttggacc actgcttaaa cagcccgtgc tgctgctcgc gctgccagtt agcaatctgc
     2641 gcgggcagcg tttcgagcca gtgtgaaaga tgatttttgg caatcagaga ataaaagtta
     2701 ccaaagtcga tcatgcagcg tcctctgctt ttaatgccac cagtgaacca aagttaaagc
     2761 actggaacca cagctcgcta tgctcaaaac cggctttatg caggcgtgct ttatgggttt
     2821 ccacggaatc ggtcagcatc acgttttcca gcatgctgcg cttctggctg atctccagtt
     2881 cgctgtaacc gttggcacgt ttaaagtcgt ggtgcatgtt gaacaacagt tcaccaactt
     2941 tggcatcttc gaaactgaat ttttccgaaa gcaccagcgc gccgcccggg ttcagtcctt
     3001 gataaatttt atccagtaat gcctggcgct cggaaggttc caggaattgc agggtaaaat
     3061 tcagcaccac catcgatgcg ttttcaatgg cgatatcgcg aatatcacct tcaatgacgt
     3121 ctactggcgt aggggcttta taggcgtcaa tatgacgacg gcagcgttca atcatcgccg
     3181 gggagttgtc gatggcaata attttgcaat tatcatgatg aatgttgcga cgcaccgaga
     3241 gcgtcgccgc gcccagagaa caacccagat cgtaaacctg cgtaccaggt tgaacgaagc
     3301 gctcggctaa catactaatc atggaaataa tattggaata gccgggaacg gaacgctgga
     3361 tcatatccgg gaagacttca gctacccgtt catcaaaggt ccagtcgccc agtctggcga
     3421 taggggcaga aaatagcgtg tcgcggtgag acataacgta aaaatccggg aaaaagaaag
     3481 tggcgtattg tgcgctaacg cagggagaaa accaactccc agggcatata ccaaagattc
     3541 gccagcacca tcagcaacag cgcacaccag gtggcgctca tgccggaacg tcgccagcga
     3601 aacagacggt gatgaaaacc gtaataatgc atcagacgac cagcaagcaa aacgatgccg
     3661 caaatatgca ccatccaggt ttctgcgcca ttcatttcca taaacagcat cagcacaatc
     3721 gcgatgggaa tatattccac cgcgttacca tgaatgcgaa tagcgctttg cagttcgcta
     3781 aaaccaccgt caccataggc aacgcggtac tgcaaacgca ag
//
LOCUS       DAZPEB010000260         3795 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZPEB010000260 DAZPEB010000000
VERSION     DAZPEB010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.265"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            232..543
                     /locus_tag="ACHURJ_003933"
     CDS             232..543
                     /locus_tag="ACHURJ_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFR1282676.1"
                     /translation="MFNVITHPAALDELLELPDNLRGRMTRLIERLEQEGNKMKMPHS
                     RVIGGGLFELRVGDKNIARTLYAYATGNEIYLLHAFVKKTQKTPTKAIEIARMRLKEM
                     N"
     gene            543..830
                     /locus_tag="ACHURJ_003934"
     CDS             543..830
                     /locus_tag="ACHURJ_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1282677.1"
                     /translation="MKVKGVPFSQVKQKLLSTPEAVKGYEDADKELEMVEMLYEMREK
                     AGLTKSALAERMGLRPSAISRLESNPLGASMKTLTKYAHACGATIDIHAVY"
     gene            1116..1430
                     /locus_tag="ACHURJ_003935"
                     /pseudo
     CDS             1116..1430
                     /locus_tag="ACHURJ_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241409.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1367..2554)
                     /locus_tag="ACHURJ_003936"
     CDS             complement(1367..2554)
                     /locus_tag="ACHURJ_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFR1282678.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQADVEPRIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVMPDALAAEGSSKR
                     EWNRFLDTHYRRGWNVNVSRVMDNATHVAVYFGSYLKKPPVPVSRLEHYAGQDEIGLR
                     YNSHRTKREEYLLMSGDEFMERFSWHVADKGFRMVRYYGFLSPAKRRLLEEVVYIITE
                     TVRKTAMQITWRGMYQRLLKVDPLKCVLCGSQMRFTGLKRGYRLAEQVLMHEPLARMR
                     WCG"
     gene            complement(2554..2919)
                     /locus_tag="ACHURJ_003937"
     CDS             complement(2554..2919)
                     /locus_tag="ACHURJ_003937"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282679.1"
                     /translation="MARSAKPRKRKPASQRSKLPRYVVKLHEDDFFDEEDAEVLRFDS
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILSGISAGG
                     MYCPECGTVHWPDGVAPPF"
     gene            3004..3333
                     /locus_tag="ACHURJ_003938"
                     /pseudo
     CDS             3004..3333
                     /locus_tag="ACHURJ_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            3363..3446
                     /locus_tag="ACHURJ_003939"
                     /pseudo
     CDS             3363..3446
                     /locus_tag="ACHURJ_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
     gene            3446..3541
                     /locus_tag="ACHURJ_003940"
                     /pseudo
     CDS             3446..3541
                     /locus_tag="ACHURJ_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(3714..>3795)
                     /locus_tag="ACHURJ_003941"
     CDS             complement(3714..>3795)
                     /locus_tag="ACHURJ_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282680.1"
                     /translation="RAIAGLNVEGLKKNARDISLLRYMPA"
BASE COUNT          975 a         1001 c          886 g          933 t
ORIGIN      
        1 aaggtgaaat ctgtcaatca gtaccggctt acgccgggct tcggcggttt tactcctgtg
       61 ccatatgcaa caacggagtg ccgccttcca tgccgctaac gcggcatcaa aaaatccctc
      121 ttctccagtt gcccccctcc gaactcagag tgtattttca tttattgatg aaatttactg
      181 tttgcgcaat tttcctgttt tagtgtattt tgtcattctg gaggctataa tatgtttaat
      241 gtgattactc acccggccgc actggatgaa cttctagaat taccagacaa tctgcgtggt
      301 cgtatgacca gactgattga acggctggaa caggaaggta acaaaatgaa aatgcctcac
      361 agccgtgtaa ttggcggagg gctttttgaa ctgagagtcg gggataaaaa tatcgcaagg
      421 acactgtacg cttatgcaac gggtaacgaa atctatctgc tgcatgcatt tgtgaaaaaa
      481 acgcaaaaga caccaacgaa agctattgaa attgccagga tgcgtctgaa ggagatgaac
      541 tgatgaaagt aaaaggtgtt ccattcagcc aggtaaagca aaaactactc agtactccag
      601 aagctgtcaa agggtatgag gatgctgaca aagaactgga gatggtggaa atgttatacg
      661 aaatgagaga aaaagcggga ttaacaaagt cagcactggc cgaacgaatg gggttacgcc
      721 cgtcagccat cagtcgcctg gaaagtaacc ctttaggggc cagcatgaaa actttgacaa
      781 aatatgctca tgcctgtggc gcaaccatcg atattcacgc tgtctattga ggggtccgct
      841 tggaattcgg aaaataaacc acgttaagac tattttttag ataaatgact ttcaggaatc
      901 gccaagatgg cgggataaca gcggttttgg tgtcctaaat cgtacaattc tgtacatcca
      961 cgcaagggaa tgtacggaag taagccagac ggttatcgga cggtcagaac atttcgtaca
     1021 gcctttacgt tcagtgagtt agctggcctg gacgtaacca ctcgttagct caattctctt
     1081 agcgtgggaa aatttccgaa ttccaagcgg acccctttat ccttgccccg ctgaatgagg
     1141 aaacgctgtt ccgggggatt atgctgaacg tcttccgttc atcgatagga attaaaaccc
     1201 caaaaagatt aaaaaacacc acaaaacgga tgtttcttca acaccacttt tgctcatatg
     1261 aacggaaccg acgattaaac tggatggctc tgattgattc agggtatgaa tggcggtttt
     1321 ttgctccgtt tccctcaaaa tggacgctac ttaccctctg cggctctcag ccgcaccacc
     1381 gcatccgggc cagcggctca tgcatcagga cctgctctgc cagacggtag ccccgcttca
     1441 gccccgtaaa acgcatctga ctcccgcaca gcacgcactt cagcgggtca accttcagta
     1501 acctctgata catccctctc caggtgattt gcatcgccgt ttttctcact gtctccgtta
     1561 tgatgtacac cacttcttcc agtaaccgcc gtttcgccgg actcagaaaa ccgtagtacc
     1621 tcaccatacg gaacccctta tccgccacat gccaggagaa cctttccatg aactcatctc
     1681 cactcatcaa caggtattct tcccgttttg ttcggtgact gttgtaacgc agtccgattt
     1741 catcctgacc ggcataatgc tccagacgac tcaccggcac cggtggcttt ttcaggtaag
     1801 agccaaagta caccgccaca tgggtggcat tatccatcac ccgggatacg ttgacattcc
     1861 agccacggcg gtaatgcgtg tccaggaagc gattccattc ccgtttactg cttccttctg
     1921 ctgccagcgc atccggcatc accaggtcag ggtatttccg tgacagtaac cgtgttatcc
     1981 ggtagcgcca catgctcatc accttacggg cgtaaaaatg aagatttttc caggtgtggc
     2041 ccgacgtcac accaccggca gttgtcgata aatggatatg cggatgccac tgctggtcac
     2101 gcccccatgt gtggatcacc gtgaatatcc tcggctccac atctgcctga tggcagattt
     2161 ccagtatcac atccgctgca atgcggctca tctctgccag caaccaccgg ttgtggaaca
     2221 ccagggacca gtactggcag ggtaacgtga acacaatatg ctgccatggg cagtcgggga
     2281 ccaggctcag cagatactgt atccactgtg cgccagcctt caccccgcag tgcgggcagg
     2341 agcggctttt acaccggaag cagacctttt ttgtatggca acagtccggt gatgaacagc
     2401 accactgtgt ataccccatc agtgtggtcc cgcacgccat gattttggtc accgactcaa
     2461 tcaccaccgg acgtactgcc ccttccggct gcttctccag ccagttaagc cagcggtttc
     2521 cctgctgaaa gatatcggca aaacggggaa gcatcagaag ggcggggcga ctccgtccgg
     2581 ccagtgaacc gtgccacact ccgggcagta cataccgccg gcgctgatac cggaaagaat
     2641 ggtcgcaaat tcccgctccg tgcagcgggc aatttccgga tacccttcgt catcaacacg
     2701 tacaaaccag aagaccagtt ttttgtttcc ggcatccaca aagaacggaa tattcaggtc
     2761 tgcgcaacac tcaacggcat catcaaaact atcaaagcgc agaacttctg cgtcttcttc
     2821 gtcaaaaaaa tcatcttcgt gaagcttcac gacatagcgg ggaagtttgc ttctttgaga
     2881 ggcgggttta cgtttacggg gtttagctga acgggccata taaccacctg aaagacaatg
     2941 acattgcctg tttttataac ggtaattgca gaccatgaca agccgcagcc gtcaggctgc
     3001 ctactcgagc ttcagctcaa taaaggcgaa tatcgacata aattaccccg atggatcttt
     3061 tttgcaaatc agggtgaatt cacgaccggt gattacgaag aaatcatgaa taaggccagc
     3121 gccctgagtc tggtatcgaa tgcaatcctg tactggaata catcaagaat aagcaatatt
     3181 gttgaagggt tacgtgaaaa tggtgaggtt attgatgatg acgtattagc ccatatatca
     3241 ttactgccct ataaacacgt attacctaat ggtacttatt ttgttgatgg agaaccttac
     3301 ggtactgagc ctaccgaaag agcaaaaccg taagtttctg ttcaaatgct cccgctggtc
     3361 catccggaca gattatcgac catcaggctg tacaggcctg ggcggacagc ctcagtactg
     3421 acaatccgtt accggtgcca cgctgatgga acagaagtgg accagtaagg cgctttctga
     3481 tttggcgcgg ttatatgatt ttctggtgct ggccagtaaa cctgctgccg ccagaatggt
     3541 acacgtatag gaaattgaaa aaccagacga taccgctgtc cacgccttat gccacgtgag
     3601 cttttctgct tttccctcca ctccggtgtt ttctctctct gcatggtcga ttcttatacc
     3661 gcaacaggct gttttttata cttgcaccga ttcccgggca cacttagcct gtgtcaggcc
     3721 ggcatatacc tcagcagact gatatcccgc gcgtttttct tcagcccctc cacattcagt
     3781 cccgcgatgg cccgg
//
LOCUS       DAZPEB010000261         3792 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZPEB010000261 DAZPEB010000000
VERSION     DAZPEB010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.266"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            65..3792
                     /locus_tag="ACHURJ_003942"
                     /pseudo
     CDS             65..3792
                     /locus_tag="ACHURJ_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014828.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT          839 a         1065 c         1312 g          576 t
ORIGIN      
        1 tgtgtcattt tatttttttg ttgctaacac agggatatga acaataacta aaagggcact
       61 ttatatgagc ggaaaaccag cggcgcgtca gggagatatg actcagtatg gcggtcccat
      121 tgtccagggg tcggcaggtg taagaattgg cgcgccaact ggcgtggcgt gctcggtgtg
      181 tccgggcggg atgacctcgg gcaacccggt gaacccgctg ctcggtgcaa aagtacagcc
      241 cggcgagacg gaccttgcgc tgcccgaccc gctgccgttc attctctccc gcacctacag
      301 cagctaccgg acgaagacgc ctgcgccagt gggaactttt tggccccggc tggaaagcgc
      361 cttctgatat ccgcttacag atacgcgatg atgcactggt actcaacgac aacggcgggc
      421 ggagcattca ctttgagccg ctgctgccgg ggaaggctgt gtacagccgc agtgagtcaa
      481 tgtggctggt gcgtggcggt aaggcagcgc agccggacgg ccacacgctg gcacgactgt
      541 gggcgtcgct gccgccggat atccggttaa gcccgcatct ttacctggcg accaacagcg
      601 cacaggggcc gtggtggata ctggggtgat ctgagcgggt gccgggtgct gaggacgtac
      661 tgccagcgcc gctgccgccg taccgggtgc ttaccgggat ggcggaccgc ttcgggcgga
      721 cgctgacgta ccggcgtgag gccgcaggcg acctggccgg ggaaatcacc ggcgtgacgg
      781 acggtgccgg gcgggagttc cgtctggtgc tgaccacaca ggcgcagcag gcggaagagg
      841 cccggcagca ggcggtcgtc gccggagcaa aacgaccgga cattcctgat tcactgccgg
      901 agagcacaga gtacggcccc gacaggggta tccgcctttc ggcggtgtgg ctgatgcacg
      961 acccggcata cccggagagc ctgcccggtg cgccactggc gcggtacacg tatacggaag
     1021 ccggtgaact gctggcggta tatgaccgca gcaatacgca ggtgcgcgct ttcacgtatg
     1081 acgcgcagca cccgggccgg atggtggcgc accgttacgc gggaaggccg gagatgcgct
     1141 accgctacga cgatacgggg cgggtggtgg agcaactgaa cccggcaggg ttaagctacc
     1201 gctatcttta tgagcaggac cgcatcaccg tcaccgacag cctgaaccgg cgtgaggtgc
     1261 tgcatacaga aggcggggcc gggctgaaac gggtggtgaa aaaagaactg gcggacggca
     1321 gcgtcacgcg cagcgggtat gacgcggcag gaaggctcac ggcgcagacg gacgcggcgg
     1381 gacggcggac agagtacggt ctgaatgtgg tgtccggcga tatcacggac atcaccacac
     1441 cggacgggcg ggagacgaaa ttttactata acgacgggaa ccagctgacg gcggtggtgt
     1501 acccggacgg gctggagagc agccgggaat atgatgaacc gggcaggctg gtatcggaga
     1561 catcgcgcag cggggagaca gtacgctacc gctacgatga cgcgcacagt gagttaccgg
     1621 cgacgacaac ggatgcgacg ggcagcaccc ggcagatgac ggcggttcac cgcgaggaag
     1681 gtatcagcct ttaccgccac tatgacaacc gtggccggtt aacctcggtg aaagacgcac
     1741 agggccgtga aacgcagtat gagtacaacg ccgcaggcga cctgactgcc gttatcaccc
     1801 cggacggcaa ccggagcgag acacagtacg atgcgtgggg aaaggcggtc agcaccacgc
     1861 agggcgggct gacgcgcagt atggagtacg atgctgccgg acgtgtcatc agtctgacta
     1921 acgagaacgg cagtcacagc gacttcagct acgatgcgct ggaccggctg gtacagcagg
     1981 gcggctttga cgggcggacg caacgttatc attatgacct gaccggaaaa ctcacgcaga
     2041 gtgaggatga ggggcttgtc accctctggc actacgatgc gtcggaccgc atcacgcacc
     2101 ggacggtgaa cggcgacccg gcagagcagt ggcagtatga cggccacggc tggctgagag
     2161 aaatcagcca cctgagcgaa ggccaccgtg tcgctgtcca ctacggctac gacgataagg
     2221 gccgcctgac gggcgaacgg cagacggtgg agaacccgga gacgggggaa ctgctgtggc
     2281 agcatgagac gaaacacgcc tacaacgaac aggggctggc aaaccgcgtc acgccggaca
     2341 gcctgccgcc ggtggagtgg ctgacctacg gcagcggtta cctggcgggc atgaagctgg
     2401 gcgggacgcc gctggtcgag tatacgcggg acaggctgca ccgtgagacg gtgcgcagct
     2461 tcggcagcat ggcaggcagt aatgccgcat atgaactgac cagcacatac acccccgcag
     2521 gccagttaca gagccagcac ctgaacagcc tggtgtatga ccgtgactac gggtggaatg
     2581 acaacggcga cctggtgcgc atcagcggtc cgcgacagac gcgggaatac ggctacagcg
     2641 ccacgggcag gctggagagc gtgcgcaccc tcgcaccgga cctggacatc cgcatcccgt
     2701 atgcgaccga cccggcggga aaccggctgc cggacccgga gctacacccg gacagcacgc
     2761 tcacggcgtg gccggataac cgcatcgcag aggatgcgca ctatgtttac cactatgatg
     2821 aatacggcag actgacggag aagacggacc gcatcccggc gggtgtgata cggacggacg
     2881 acgagcggac ccaccactac cactacgaca gccagcaccg cctggtgttc cacacgcgga
     2941 tacagcatgg cgagccactg gtcgagagcc gctacctcta cgacccgctg agccgccgga
     3001 cggggaaacg ggtgtggcgt cgcgagcgtg acctgacggg gtggatgtcg ctgtcgcgta
     3061 aaccggaggt gacgtggtac ggatgggacg gcgaccggct gacgacggta cagaccgaca
     3121 ccacgcgtat ccagacggta taccagccgg ggagcttcgc gccgctcatc cgcatcgaaa
     3181 cagacaacgg tgaacgggag aaagcgcagt gccgcagcct ggcggagaag ttacagcagg
     3241 aagggagcga agacggtcac ggtgtggtgt tcccggctga actggtgggg ctgctggaca
     3301 ggctggaggg ggaaatccgg gcaaactgcg tgagcagtga aagccgccag tggcttgcgc
     3361 agtgcgggct gacggtggag cggctggcgg cacagataga gccggtttat ttgcctgagc
     3421 gaaaaataca tctttatcac tgtgaccacc ggggcctgcc gctggcgctt atcagtgaag
     3481 acggcaatac ggcgtggagc gcggaatatg atgaatgggg caaccagctt aatgaggaga
     3541 acccgcatca cctgcaccag ccgtaccggc tgccggggca gcagtatgat aaggagtcgg
     3601 ggctgtacta caaccggaac cggtactacg atccgttgca ggggcggtat atcactcagg
     3661 acccgatagg gctggagggg ggatggagtc tgtatgcgta tccgctgaat ccggtgaatg
     3721 gtattgatcc attagggtta agtcccgcag atgtagcgct aataagaaga aaagatcaac
     3781 taaaccatca aa
//
LOCUS       DAZPEB010000262         3737 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZPEB010000262 DAZPEB010000000
VERSION     DAZPEB010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.267"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..860)
                     /locus_tag="ACHURJ_003943"
     CDS             complement(<1..860)
                     /locus_tag="ACHURJ_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFR1282681.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESAIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAE"
     gene            complement(885..1763)
                     /gene="yihU"
                     /locus_tag="ACHURJ_003944"
     CDS             complement(885..1763)
                     /gene="yihU"
                     /locus_tag="ACHURJ_003944"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFR1282682.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQG
                     ATAAQTQAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQT
                     DALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARETGTPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            1936..2832
                     /locus_tag="ACHURJ_003945"
     CDS             1936..2832
                     /locus_tag="ACHURJ_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFR1282683.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPEGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYNLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTKEAIRFASAVAAMKCT
                     QPGGRAGIPNREQTESFLSLYA"
     gene            2872..3660
                     /locus_tag="ACHURJ_003946"
     CDS             2872..3660
                     /locus_tag="ACHURJ_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR1282684.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDLNEALVARTMIKHARNTLLVADHTKFAA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
BASE COUNT          962 a          955 c          902 g          918 t
ORIGIN      
        1 atttctgcca accgttgtaa acgaggaaca gagacatcgc gcagcatagt ttgtggatct
       61 tgtgcaccaa caaccgacgc ccaaaccgct cgtccagcca ggaagcccga cgcaccacct
      121 ttcatggcaa tacttactgc acggccaaaa atatcggcat caacgccaga agaaagaatt
      181 acccacggca tcttcatctg atcgttaagt tgctgacagg cggcaagtaa ggttttttca
      241 tcgcctttgc cccctaatgg catctcggct ttgtaaagat cagcctctgt tccaccaagc
      301 tctgcggcgg cggcaacaat ggcgctttca cgatcaaagt cccaaccgcg acgaggtgga
      361 cgcaccacag gttcaataat gctcaccagc ccggcactac ggcagcgacg aacaaattta
      421 tcaaccattg ccagacgttc ctctgcgggt tcatcttcac gccagagtac aagtaatttc
      481 attgccttag cccccatttc acgggcttta tgcgggtcga cggacatatc tatttcaacg
      541 ctatcaaccg ggatgccatt acctgggata aaaagatcgg cagcgacaat caggccgcaa
      601 gaattagcaa cagcgccctg ctcgactatt tgctcaagac aaaattgttt atccgccaga
      661 acggcagaag cataaggaga aaggattcgg gtcgcggcaa ctttaaaatc ggtgagtaca
      721 ctgtcggcga tgggttttgg ctgccctgct gccgcaaaca tcaagcgcat ggcctcacgc
      781 tgatcgacag ctaacatggc aaatccacca cctgggcggg agatatcttt cagcgtaaat
      841 ggggtgaagt gggatgacat agaaactcct gttaatagat aaaattaaag accagcctgc
      901 tggcgaacct gatttagcag ggcactccag tcctgccgcc ctttgccgtc ttgacttgct
      961 gcagcgtaat aactttctgc tgcaatcccc atattgagcg gtgtccccgt ctcccgagcg
     1021 acgtcaactg caatacgcag atctttcaac gccagatcca ccatgaaggc gggtgaaaga
     1081 tcgcctttca gcactttgcc aggccaggtg gtcgtaaaat gtcctttccc tgctgccgta
     1141 ccactcatca ccttaattgc gacatcaaga tttaatccca gtgactcaca taacacagcg
     1201 gcctcggagg acagcgcatt aagcgcaatg ctcatatagt tgttaattag cttcacgcgg
     1261 atccccattc ctgggccgcc agcctcgacc agctcattac ccatgcacat aagaatcggg
     1321 cgtgcacgtg caatctgctc atcagtgcca cccgctaaaa tcagcaatgt gccatcaatc
     1381 gcattcacgg acgttctgcc cacgggagcg tccatcatac tgatgccttt ttcttgtaac
     1441 tcccgtatta atgcatcggt ctgtaaggga tgaatagtcg acatatcaat caataacgcg
     1501 tcagatgaaa cagcctcaca gacgcctttt tcccccaaca gaacctgtcg aacaatgtca
     1561 ccattgggaa gcatagtgat gataaattca gcgcctgctg ctgcttccgc ttgggtttgt
     1621 gccgcagtgg ccccctgcgt aactagcgca tcgacggcct gtgcattgac atcaaatacc
     1681 tgcaatgaat gccctttttg caatagattg ttagccattg ggctacccat ctgccccagc
     1741 ccgagaaatg caatggttgc catgaatact ctcctgtctg acattgacaa aataatgaca
     1801 attgaaaatt cgtttttgtc tgtttttgag catatttgtg atttatgttc aaaaacacga
     1861 cacgcatcac taatttgatc atttgcgtca tttaaattga ccaaagacga caaaattaaa
     1921 caggacaaga caaatatgac cagaattgca tgcgtgggta tcacagttca ggacagaatt
     1981 tattctttgc ccactctgcc ggagggcggc gggaaatatc aggcaaatca ttatcttgag
     2041 attggtggcg gcccggcagc aacagccgct gttgcaatcg caaagcttgg cgtggaggtt
     2101 gatttcatcg gtcgcgtcgg cgatgacagt tgtggaaata ccttacttgc agaacttgag
     2161 ggttggggcg tcaataccgc attttgccgc cgatatccta acgcccgttc gtcgcaatca
     2221 gcgatcctcg ttgatcagca tggcgaacgg attatcgtta attatccaag tcctgatctc
     2281 ggtactgatg ccgaatggtt agaagcgatc gatttctctc gttataatct gattctggcc
     2341 gatgttcgct ggcatagcgg tactgaaaaa gcattctcgc tagcgcgcct tgcgggtgtg
     2401 acaacgcttt tagacgccga tatgacaccg caggatattt ctccacttgt cgcgcttgcc
     2461 gatcacgccg tgttctcaac accagggctg aaacgcatga ctggcctgca gtctcctgaa
     2521 gaaggtttgt tccaggcaac gacacaaaca gcaggtaaag tgtatgttac gctgggttct
     2581 gaaggatcgt tgtggattga agatggtcat ttgtgtcaac aggaggcatt ttctgtcaat
     2641 gtggttgata ccactggagc aggcgatgtt tttcacggcg cactcgcggt agcgttggca
     2701 gaaaaaatgc caacaaaaga ggctattcgt tttgccagtg cggttgccgc gatgaaatgt
     2761 acccagcctg gcggtcgggc cggcattcct aatcgtgagc aaaccgaatc atttttgtca
     2821 ctttatgcgt aaaatggtaa acatctaccg aggaggggca agggagtatt catgagtatc
     2881 atcgaagtga caggtaatcc acgccatgac caactcgttc atctaatcgc agaacgcggt
     2941 tatatgaata ttgaagaact ggcacaactg ctggatgttt caacgcaaac cgtgcgccgg
     3001 gatatccgaa aactcagtga gcagggcttg atcacccgtc atcatggagg ggccgggcgc
     3061 gtctccagcg tcatgaatac cgcttttgag caacgggaac tttcgctcac cgccgagaaa
     3121 cgggcgatcg ctgaggcagt cgccgattac cttcccgaac gctgtaccgt ctttatcacc
     3181 atcggtacaa ccgtagaagc cgttgccagg gcattactca accggcgtga tttacgcatt
     3241 atcactaaca gcctacgtgt ggcacagatt ctttataaga atcaggatat tgaagtgatg
     3301 gtgccgggag gcactttacg cgctcataac ggcgggatta tcggcccagg agccgtggat
     3361 tttattgaag gcttccgcgc agattattta atcaccagta taggtgccat tgaacatgac
     3421 ggcaccctac tggaatttga tctaaatgaa gcgttagtcg cgagaacgat gattaaacat
     3481 gcgcggaata cattgttagt ggccgatcat acgaagtttg ccgcgtctgc tgccgtttca
     3541 attggcaatg cacggaatgt cagggctttt tttactgatg ccccgcctcc caattctttc
     3601 tgccagttgt taagtgaaga gaatgttgaa ctggtggttg ccgagcaaga agtatcctga
     3661 aattgaaatt tcggaaaatc cgctgggaat ttcagcggat tttgttttcc ataagcaaaa
     3721 aaaacctgcc agcgatg
//
LOCUS       DAZPEB010000263         3730 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZPEB010000263 DAZPEB010000000
VERSION     DAZPEB010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.268"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(118..2187)
                     /gene="glyS"
                     /locus_tag="ACHURJ_003947"
     CDS             complement(118..2187)
                     /gene="glyS"
                     /locus_tag="ACHURJ_003947"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFR1282685.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLVESFAANFTVELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYACHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2197..3108)
                     /gene="glyQ"
                     /locus_tag="ACHURJ_003948"
     CDS             complement(2197..3108)
                     /gene="glyQ"
                     /locus_tag="ACHURJ_003948"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFR1282686.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3203..3502)
                     /locus_tag="ACHURJ_003949"
     CDS             complement(3203..3502)
                     /locus_tag="ACHURJ_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFR1282687.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
BASE COUNT          864 a         1006 c          960 g          900 t
ORIGIN      
        1 cataaaatta atatatgaga taatctttat tcagcagaag attattaaag gttgctgtat
       61 tatttagcga taataaaaaa agcctgccag atggcaggct ttttaataac ggcgttatta
      121 ctgcaacagc gaaatatccg caacgcgcag gaacagttcg cgcagtttct ccagcatggt
      181 cagacggttg agacgcaatt ctttgtcatc aaccatgacc atcactttat cgaagaaagc
      241 atcaaccggc tcacgcagct ctgccagttc gaccagcgca tcctggtaac gaccttccgc
      301 aaagtacggc tccagcttgt cacgtagcac cacaacctgc atcgccagtt taatttcttc
      361 cggctctttc agggtagagg cattcacgcg gtcgctcagc acttcgtcag atttcgccag
      421 aatgttagat acacgtttgt tcgccgccgc cagtgcagca gctgcatcca gggtacggaa
      481 atgcgatacc gctttcatac gggcatcgaa atcagccgga cgagtcggac gacgcgccag
      541 taccgcctgg atggtgtcaa cggtgtaacc ttcgtcctga taccaggcgc ggaagcgacc
      601 gagcataaag tcgataacat catcaaccac gttggcgtta gtcagcttat cgccatacag
      661 acgcactgct tcttcggtca gcgtttgcag atcaaggttg aggttcttct caacgataat
      721 tcgcagcacg ccaagtgcgg cacgacgcag cgcaaacggg tctttgtcgc ctttcggatg
      781 ctgaccgata ccgaagatac ccgccagagt atccatctta tcagcaatcg ccagcgcaca
      841 agctaccggg ttggacggca ggtcatcacc ggcaaagcgc ggctgatact gctcattcag
      901 cgcgacggct acgtcttccg cttcgccatc gtgacacgcg tagtgcatcc ccataacgcc
      961 ttgggtgtcg gtgaactcga agaccatgtt ggtcatcagg tcacacttag acagcaggcc
     1021 cgcacgggtt gcgtggttaa cgtcagcgcc aatctgttca gcaatccagc cagccagcgc
     1081 ctggatgcgg tcagttttgt cacgcagcgt acccagctgt tgctggaaca gcacggtttg
     1141 caggcgcggc aggttatctt ccagacgttt tttacggtcg gtattgaaga agaactcggc
     1201 atccgccaga cgtggacgaa cgactttctc gttaccagag ataatttgct gcggatcttt
     1261 cgattcgatg ttggcaacga agatgaagtt cggcagcagt ttgccatcgt tcgcatacac
     1321 cgggaagtat ttctggtcac ctttcatggt gtaaaccagg gcttcagccg gcaccgcgag
     1381 gaatttctct tcgaattttg cggtcagaac gaccggccac tccaccagcg aagccacttc
     1441 ttccagcagg ctttcgctta agtcagcgtt accgccaatc ttacgcgctg cttcttcggc
     1501 atcggcttta atcttcgctt tacgttcttc gtaatcggcg atgactttcc cgcgctcacg
     1561 cagaatttcc ggatactgat cggcgttgtc gatggtgaat tccggttcgc ccataaagcg
     1621 gtggccgcga atcacgcgat cggactgaat gcccagaata gttgccggaa tgactttgtc
     1681 gcccagcagc agggtcacgg tgtgcaccgg acgcacgaag tgcacgtcgc ttgcgcccca
     1741 acgcatcagt ttcgggatcg gcagtttcgc cagagaagtc gcaaccatat tcggcagcag
     1801 tgcttcggtg ctttcgccct tcacatgggc gcgatacagc agccattcgc ctttatcggt
     1861 agtcagacgc tcagcctggt caacggtaat accgcaacca cgcgcccaac cttctgccgc
     1921 tttgctcggt ttgccttcag cgtcgaacgc ctgggcaatc gccgggccgc gtttttcgat
     1981 ttcacgatcc ggttgcgctt ccgccaggtt agctactttc agcgccagac gacgcggagc
     2041 agcaaaccat tgaacggtgc cgtgtgcgag gccagcgtta tccagctcca cagtaaaatt
     2101 cgcagcaaag gactcaacca ggctgcgcag tgcttttggt ggcagctctt cagtgccgat
     2161 ttccaccaga aaagttttct cagacatagc cgcctcttac ttatctttgt tgcacatcgg
     2221 gaagccgagg gcttcacggg aagcgtagta tgcttctgcc actgctttgg tcagggtgcg
     2281 aatgcgcaga atgtagcgct gacgctcggt gacggagatg gctttacgcg catccagcag
     2341 gttgaagctg tgggcggctt tcagaatacg ctcgtaggct ggcagcggca gcggattttc
     2401 cagcgccagc agctgctgcg cttctttctc gtactgctca aagcaggtga acaggaagtc
     2461 cacatccgcg tattcgaagt tgtaagtgga ctgctccact tcgttctgat ggaacacgtc
     2521 gccgtaggtg gttttaccca gcgggccgtc gctccagacc aggtcgtaaa cgctgtctac
     2581 gccctgaatg tacatggcca gacgttccag accgtaggtg atctcgccgg taaccggttt
     2641 acactccaga ccaccaacct gctggaagta agtgaactgc gtcacttcca tgccgtttag
     2701 ccacacttcc cagcccagtc cccaggcacc cagagtcggg ttttcccagt tatcttccac
     2761 gaaacggatg tcgtgaatag tcgggtccat gcccagctct ttcagagaac cgaggtacag
     2821 ctcctgaata ttgtccggcg atggcttaat gaccacctgg aactgatagt agtgctgtaa
     2881 acggttgggg ttttcgccgt agcgaccatc ggtcgggcga cgagaaggct gaacataagc
     2941 agccgccatc ggttctggcc ccagcgcgcg cagacaggtc attgggtgag aggttcccgc
     3001 gccgacttcc atgtccaatg gttgaacaat ggtgcagccc tggcgagccc agtaatcctg
     3061 taaggtcagg atcaagccct ggaaggtcct ggtatcaaac ttttgcatat tatttcgtgc
     3121 tggatacgtg tggatttaaa ggaagggatc agtatacccg ctggatggaa gatatacagt
     3181 acgaaacggg aaaaagcagg gcttaacgca tggaaagatg caaaaaatgg ccatctgcgt
     3241 caaaagaaca gacaaactgc tctttacgga aggtataacc gcgcatttca tagcttccct
     3301 ggaactgttc gaaggcggtg acgtcgattt tttgctcgcc ggtgttataa cgttttgccg
     3361 cctgatcctt gcataacgct tccatattca acgaacgcag aggatcaacc tttacccgtt
     3421 gtgctttctg cacgggtgac ggtgtagaac aacccactag caccataagc gccattgccg
     3481 gaaagaaagc gttcatcatc attaaattac cgtctggttt gcaggtgagt cttattattt
     3541 atatcggtat agtggctgat atcttacgaa tttccttaag cctctataag ctgtagtgat
     3601 aaaactcaga ttattccagt gcgccaacca aatgatttat cggtcagact ttttttaacc
     3661 agatacagag acacagatgc aggtaatgct gccaacttac tgatttagtg tatgatggtg
     3721 attttaaggt
//
LOCUS       DAZPEB010000264         3724 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZPEB010000264 DAZPEB010000000
VERSION     DAZPEB010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3724
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.269"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            37..264
                     /locus_tag="ACHURJ_003950"
     CDS             37..264
                     /locus_tag="ACHURJ_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFR1282688.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(265..639)
                     /locus_tag="ACHURJ_003951"
     CDS             complement(265..639)
                     /locus_tag="ACHURJ_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFR1282689.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(722..1946)
                     /gene="mdtG"
                     /locus_tag="ACHURJ_003952"
                     /pseudo
     CDS             complement(722..1946)
                     /gene="mdtG"
                     /locus_tag="ACHURJ_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
     gene            complement(2118..3038)
                     /gene="lpxL"
                     /locus_tag="ACHURJ_003953"
     CDS             complement(2118..3038)
                     /gene="lpxL"
                     /locus_tag="ACHURJ_003953"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFR1282690.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3263..3724
                     /locus_tag="ACHURJ_003954"
                     /pseudo
     CDS             3263..3724
                     /locus_tag="ACHURJ_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415573.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
BASE COUNT          915 a          952 c          945 g          912 t
ORIGIN      
        1 cgttatgccg gtattttttt acggaagagg tatcaaatgc gtttaattgt ggtgagcatc
       61 atggtgacct tgctgagtgg ctgtggcagc attattagtc gcactatacc ggggcagggg
      121 catggcaacc aatattatcc cggtgtgcaa tgggatgtgc gtgactccgc ctggcgttat
      181 gtcacaatcc ttgatctgcc attctccctg gtttttgata ctttactgct gccgatcgac
      241 attcatcatg gcccgtatga gtgattaacg ttcatcccac tcatcagctg ctgcgcgtcc
      301 ttcctcggta tccagcggtg gctcaagctg aaattccccc tcgtcccatt catgtaatgt
      361 attctcttcc tgccactcct ggcgtatctc tatctcatca tagtcgccat caaaaacact
      421 ttgcgcggct tcaccgctaa gcataggtaa acattcacct tcttcccctt cgtcggcaaa
      481 aaactcaact tgccacatga tgtcgccgtc ctgcaaaacg tatttttggg cattgaactg
      541 ttgcacattc gcatcttcgg cgtcgatgcc ggggttgtct gcaagaaatt cttcgcgtgc
      601 agcgtcaatg gcttcttcaa gcgttgcgta catggtcatc acgattctcc ctttgagttg
      661 atgaggtttc agggaaaagg atagctgatt ctccgctttt gcaagtatga aaggcgaaaa
      721 atcagttcga tacctggggt attcgacgac gacgtagact gttccatgaa tagactgcgt
      781 tgaataacac tacgccagcg gtgacgagaa ataccgctct gaaaccgtag ttcgctgaaa
      841 tcgctgctcc catcaatggt ccggtaacgt tgccaatatc acgaaacgat tggttatagc
      901 tgaagatacg cccggcgatc tggttgctcg agttgtaaac caacaatgtc tgtacggcgg
      961 ggagtagtgc accatcggcg gcaccgagca aaaaacgtaa aatcccaagt tgcaatggcg
     1021 tctgaacgta agacattggg atcaacagca gtacagaaaa gatcagcgct gtaatcagga
     1081 tcttttcggg tccgattcga tcgccaagtt tgccgagtcg tggtgcactt agcagagccg
     1141 ccacgcctgg caccgaggcg atcatgccac tgataaaggc gacgttactg acgttacccg
     1201 ccagttcgcg gacatacagc gtcagaatgg gggcaattga gcccgtcgcc acctggatga
     1261 ttaacgtagt gacaaacagg ctgagtacca gtttcgggtt tttaagtgat gtcaccactt
     1321 cccgcatgtg cagcatctct tttttgctga ccggctggaa tttttctctg atgcaaaaca
     1381 gggtgacgaa aaagcagagt atgagcacac tggtggtaat aaagaatacc ggacgtaagc
     1441 cgtagctatc ggcgagcagg ccgccagcca ttgggccgag caacgcacca ccaacgccgc
     1501 ctgtggagag cgtacccagc gcccagccgc ttttattacg cggtacttgt gtggcgataa
     1561 gagcattagc gttggggaca aatccgccaa gtaacccaag aagcgcccgc aggatcaaaa
     1621 actgccagat attttgtgcc agccccatca acaccatcac gatgcccatg ccgagggcag
     1681 agcgtaatag catgagtttt cggcctttac ggtcggcgag tccaccccaa aacggtgagg
     1741 cgatggccga aaataaaaat gtaatgctga agacaatacc ggaccatatt cagggcggag
     1801 tgaccggtaa cgccaagctg ctcaacgtag aggggtaaga agggcattac cagactgaag
     1861 gcggcaccgg taagaaaaca gcctagccag gcgacgatca ggtttcgttt ccagtttata
     1921 ggggtgtcat tttcacaggg tgacatagca atccgctgtt ggtgcgccag gcgcggtgaa
     1981 cataagaaga aaagataagc acactaatta tgcgcccgac ttccaggggg cgcaatccag
     2041 agagctttta tcgctaaatc agggggattt gctgtggtaa tgccggatgc cattctgaag
     2101 catccggcag gggagattta atagcgtgaa ggaacgcctt ccgggcgtgt tttaaagcga
     2161 cggtgtaacc acatatactg ctctggtgcc atcatgatgc atttttcgac cactttgttc
     2221 atccacgcgg cggtagtttc ggcatcatcc agtggcggag aacactctgg cggcagcata
     2281 atcaattgat accctttgcc atctggctta cggcgtggaa cgaagggcac cagacatgcg
     2341 ccggacatcc gtgccagcat ccaggttccg gtcgtggtcg cagcctgctc aacggcaaat
     2401 aacgggacga aaacgcttga gcgcgggccg taatcatgat ccggtgcgta ccagaccact
     2461 tcgccttttt tcagggcttt aatcatgcct tttaaatctt tgcggtcgag catcgattta
     2521 tttgagcgca aacggcccca ggtttgtagc cagtcaatca gtggattatc gttcgggcgg
     2581 taaacgccaa tccccggttc ctgcatacca aactgccgcg cacccagctc cagtgtcaga
     2641 aaatggatgc caactaacag gatgccgcgt ttttgcgcct gcacgtcacg aatgtgttcc
     2701 atgccgatca cttccgtcca gcgcgcgatt cggcggtccg gccagaacca cgccatgccg
     2761 gtttccatca ggcccatgcc aacggattcg aaattcttca ccaccatttt acggcgttct
     2821 tgttcgctca tttccgggaa gcacagttcc aggttgcgat gcacaatttt tgcgcgtcgt
     2881 ttcataaaac gtaacgccag ttttcctaat ccacaaccga ggcggtagat aaccgggtag
     2941 ggcaattgca cgactaacca aagtacgcca atacccaacc aggttaacca ataacgcgga
     3001 tgaagcagtg cggtggagaa cttgggtaga ttcgtcatat caatcctgtt tttcaaccta
     3061 ttcgggcaat tgtatgtatt gtcgcatttt ttcgcccgca accaaaattt gtggctgaag
     3121 actgggcgaa attgccgcgc ttgtaaataa caaataattt ttaatgcgca aatgtagcgt
     3181 aaaatgtgtg gatgttaatt atcgataatt gctatatcat gccgcggatt tttactttcc
     3241 catctcgcag gaaccgtaca ccatgccagt gttacacaac cgcatttcca acgacgcgct
     3301 aaaagccaaa atgttggctg agagcgaacc gcgaaccacc atttcgtttt acaagtattt
     3361 ccacatcgcc gatcctaagg cgacccgtga cgctttatat cagctgttta ccgcgctgaa
     3421 tgtttttggg cgagtgtatc tggcgcatga gggcattaac gcgcaaatca gcgtacctgc
     3481 gagcaatgtt gaaacatttc gcgcgcagct ctgccttcga cccggcttta gagggtttac
     3541 gcctgaatat cgcgttggat gatgacggga aatccttctg ggtactgcgc atgaaggtac
     3601 gtgatcgcat tgttgccgac ggtattgacg atcctcactt tgatgccagc aatgttggtg
     3661 agtatctgca agcggcggaa gtgaacgcca tgcttgacga tcccgatgca ctatttatcg
     3721 acat
//
LOCUS       DAZPEB010000265         3682 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZPEB010000265 DAZPEB010000000
VERSION     DAZPEB010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.270"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..213
                     /locus_tag="ACHURJ_003955"
     CDS             <1..213
                     /locus_tag="ACHURJ_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFR1282691.1"
                     /translation="LYDGRTLVQTVTGSLACGVLAMAVAGSLRFFGFPEDAVTFIGAS
                     IGFMGAEKARDKVIAAFNRRVKEKDE"
     gene            210..602
                     /locus_tag="ACHURJ_003956"
     CDS             210..602
                     /locus_tag="ACHURJ_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFR1282692.1"
                     /translation="MSNTFKFSSRSEKNLQGVNPDLVKVTRRALEISEVDFGITEGLR
                     SRYRQKQLVATGKSQTMNSRHLTGHAVDVVAYIGSQMSWEWPLYEKIAAAFRQASREL
                     NIPVEWGGDWKTLKDGPHFQLPHGAYPA"
     gene            599..1006
                     /locus_tag="ACHURJ_003957"
     CDS             599..1006
                     /locus_tag="ACHURJ_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HFR1282693.1"
                     /translation="MKLWPTLGVAFLLTAGWGTSMRLSWSLGRENARNEAQASTLKST
                     ADTLNIISAGVQDMQQVLAQLRVENQQRNQDGEVRREQLRNDIAKDECAHALPDARFT
                     DRLRRHAERATASAVSPAYTADADHTGNASPLP"
     gene            1144..1611
                     /locus_tag="ACHURJ_003958"
     CDS             1144..1611
                     /locus_tag="ACHURJ_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1282694.1"
                     /translation="MLKTDSLREAMTRSCRWCQANPEKFTIFVESGNIETTGETPSFV
                     YRYQMVMFVMDYAGELDDLTLPLLAWLSENQPQLLLNPERNQDIKFSAVINDDDSADL
                     LFTLPLRERVRITRSSQGTPQAEHLLEPKPRLPSSEGDWSHVFQDVTWGESDG"
     gene            1604..2239
                     /locus_tag="ACHURJ_003959"
     CDS             1604..2239
                     /locus_tag="ACHURJ_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFR1282695.1"
                     /translation="MDKAFTRVDETFEAIRDSLNQQAINNIARKLAQDLRRAQQARIR
                     PQKAPDGTAWTPRRRHVTRIQERIRFIWNNEARTLKNWHHDTGKYGRTITGWDEDKNS
                     IRTFYRDDIDRFLEIRTRRINQDSTKRVPMFVKLRTARYLKARADASGVTVGYSGVAA
                     RIARVHQFGERDQVAPGIFTDYPVRELLGISQADERLIYNTVLGRIAEAVR"
     gene            2251..2817
                     /locus_tag="ACHURJ_003960"
     CDS             2251..2817
                     /locus_tag="ACHURJ_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFR1282696.1"
                     /translation="MRLLSNIIRTGIISEVDEKSWRVRVRSGELETGWLRWNTSRAGA
                     FNVWLPPSPGEQVVIACIGGNPETAMIIGSLWSDAIPAPGKSLKEIVVSAPDGAVFRY
                     DADAGALSASGMKTATLQASVSVTLDTPVVECTDLLRTATLDVTKGGKMSGNITHSGG
                     NFTSNGITVHTHKHGGVKGGSDSTGGPQ"
     gene            2814..3164
                     /locus_tag="ACHURJ_003961"
     CDS             2814..3164
                     /locus_tag="ACHURJ_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFR1282697.1"
                     /translation="MTTRYTGMNPDGTGNLNDMEHLKQSVRDILTTPLASRVMRREYG
                     SLVPDLIDEPMNNTTRLQCMSAAVIALTRWEPRIALDAIDVVWKAGGRAGVTLSGTVM
                     QTMQNVELTITLRE"
     gene            3168..>3682
                     /locus_tag="ACHURJ_003962"
     CDS             3168..>3682
                     /locus_tag="ACHURJ_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HFR1282698.1"
                     /translation="MPAVDLSQLPEPAIIAEPDFEAILADTKAMMIASYPAEQREAVS
                     AALELESEPLNVIAQTMSFREMLLRQRVNEGARACMLSHSAGTDLDNLAGNMNTKRLV
                     ITPATDTTDAVMESDTSLRLRAQRAYDGLSVAGPSGAYEYFARSASGLVRDARAISPS
                     PANVTVSILST"
BASE COUNT          887 a         1035 c         1077 g          683 t
ORIGIN      
        1 ctttatgacg gacgaacact ggttcagacc gtaacgggat cgctggcgtg cggcgttctt
       61 gccatggccg tggccgggtc gttgcgcttc ttcgggtttc ctgaagatgc cgtgacgttt
      121 atcggcgcat caatcggttt tatgggtgca gagaaagcac gcgacaaggt tattgcggcc
      181 tttaatcgca gggtgaagga gaaggacgaa tgagcaacac atttaaattc agcagccgga
      241 gcgaaaagaa tttgcagggt gtaaatcctg atctggtgaa agtgacccga cgggcactgg
      301 aaatctcgga agtggatttt ggtatcaccg aagggttgcg cagccgttac cgccagaagc
      361 aactggtggc cacgggtaag agccagacca tgaacagccg ccaccttacg gggcatgccg
      421 tggatgttgt ggcttatatc ggcagccaga tgtcatggga atggccgctg tacgaaaaaa
      481 tcgcagcagc attcagacag gccagccggg aactgaatat tccggtggaa tggggcggcg
      541 actggaagac cctgaaagac ggaccgcatt ttcagttacc acacggagcc tatccggcat
      601 gaagctctgg cccacgcttg gcgtcgcttt ccttctgact gccggatggg gaacatccat
      661 gcgtctgtcg tggtcgctgg gccgggagaa cgccagaaac gaagcgcagg ccagcaccct
      721 gaaaagtacc gccgacaccc tgaatatcat cagcgccggg gtacaggata tgcagcaggt
      781 gctggcgcaa ctccgcgtgg aaaatcagca acgcaatcag gacggagagg taagacgtga
      841 acagctacgc aacgatattg caaaagatga atgcgcccac gctttgcctg acgctcgttt
      901 tactgacagg ctgcgcaggc acgcagaacg cgccactgcc agcgccgtca gtccggctta
      961 taccgcagac gctgaccata ccggtaacgc ctcccccctt ccctgacact cccacatggg
     1021 gaaatctcgg tatatggggc gaccgccttc tggatgcact ggaaacctgt aacgcggata
     1081 aacgggccat tgaattactg gaacagcgca ggctgcaacg actgaacaac gaggataaca
     1141 accatgctga aaactgattc cctgcgtgaa gccatgaccc gttcatgccg atggtgtcag
     1201 gctaacccgg aaaaattcac cattttcgtg gagagcggca acattgaaac gaccggagaa
     1261 accccatcgt ttgtttaccg ctatcagatg gtgatgtttg tcatggatta cgccggggag
     1321 ctggacgacc tcacgctgcc gctgctggcg tggttatccg aaaatcagcc acagttgttg
     1381 ctcaaccctg agcgtaatca ggacatcaaa ttttccgccg ttatcaatga cgatgacagc
     1441 gccgatctcc tgtttacgct ccccctgcgg gaacgcgttc gcatcacgcg cagcagtcag
     1501 ggcacaccgc aggcagaaca cctgctggag ccaaaacccc gcctgccatc ttccgaaggc
     1561 gactggtcgc atgtattcca ggatgtgacg tggggtgaaa gcgatggata aggcattcac
     1621 tcgcgtggat gaaacctttg aggccatccg cgacagcctg aatcagcagg ccatcaataa
     1681 catcgccaga aagctggcac aggatttacg ccgcgcccag caggcacgta tccggccaca
     1741 gaaagcgccg gacgggaccg catggacacc acgcagacgc cacgtaaccc ggatacagga
     1801 gcgcattcgc tttatctgga ataacgaagc acgcacgctg aaaaactggc atcacgacac
     1861 ggggaaatac gggcgaacca tcaccgggtg ggatgaggat aaaaacagca tccgcacgtt
     1921 ttaccgggat gacatcgacc gctttctgga aatacgcacc cggcgcatca accaggacag
     1981 cacaaagcgc gtccccatgt tcgtaaaact gcgcaccgcc cgctacctga aagcccgtgc
     2041 agatgcttcc ggtgtgacgg tgggttacag cggcgtggcc gcacgtattg cacgcgttca
     2101 tcagttcggt gagcgcgatc aggttgcgcc gggcattttc accgattacc cggtacgtga
     2161 gctgctgggt atcagccagg cagatgagcg cctgatttat aacacggtgc tgggccggat
     2221 tgcggaggct gtacggtgag cgcagaactc atgcggctac tgagcaatat catccgcacc
     2281 gggatcatct ctgaagttga tgagaagtcc tggcgcgtgc gcgttcgcag cggcgaactg
     2341 gaaacaggtt ggctgcgctg gaacacctct cgcgcgggag ccttcaatgt gtggctgccg
     2401 ccatcaccag gcgaacaggt ggtaattgcc tgcattggcg gcaacccgga aaccgccatg
     2461 ataattggca gcctgtggag tgatgccatt ccggcccccg gcaaaagcct gaaagaaatc
     2521 gtggtcagcg cgccggatgg cgcggtgttc cgctacgacg cggacgcagg cgcactgagc
     2581 gccagcggca tgaaaacagc caccctgcag gcatccgtca gcgtgacact ggatacgccc
     2641 gtcgtggaat gcacagacct tctgagaacg gcgacgcttg acgtcacaaa agggggaaag
     2701 atgagcggca atatcacgca cagcggcggc aatttcacct caaacggcat cacagtgcat
     2761 acgcataaac acggtggcgt taaaggtggc agcgattcga caggaggccc gcagtgacaa
     2821 cccgctacac aggaatgaac ccggacggaa cgggaaacct gaacgatatg gagcacctga
     2881 aacagtcagt cagggacatc ctgaccaccc cgctggcaag ccgggttatg cgacgggaat
     2941 atggcagcct tgtgcctgat ttgattgacg aacccatgaa taacaccacg cgtctgcaat
     3001 gcatgagtgc tgccgtgatt gcgctgacac gatgggaacc ccgcattgcc ctggacgcta
     3061 tcgacgttgt ctggaaggca ggaggccgcg ccggggtgac gctgtcgggc actgtcatgc
     3121 agaccatgca gaatgttgaa ttaaccatca cgctgaggga gtaaatcatg cctgccgttg
     3181 acctttcaca gttaccggaa cccgccatca tcgcggagcc tgactttgag gcaattctgg
     3241 ctgacacaaa ggccatgatg attgcgtcct atcccgccga acagcgtgaa gccgtttccg
     3301 ccgcgctgga gctggaatcg gaacccctta acgttatcgc tcaaaccatg tcgtttcgtg
     3361 aaatgctgtt acgccagcgg gtcaatgagg gtgcacgcgc ctgcatgcta agccacagcg
     3421 ccgggacaga cctggacaac ctcgcgggca atatgaacac aaagcgcctg gttatcactc
     3481 cggcaacgga taccaccgat gcagtgatgg aaagtgacac ctcgctgaga ctacgggcgc
     3541 aacgggcata tgacggtctg agtgttgctg gcccgtcagg tgcatacgag tattttgcac
     3601 gcagcgccag cggtctggta cgcgatgcgc gggccatcag cccgtctccg gccaacgtga
     3661 cggtttccat cctgtccact ga
//
LOCUS       DAZPEB010000266         3681 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZPEB010000266 DAZPEB010000000
VERSION     DAZPEB010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.271"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..213
                     /locus_tag="ACHURJ_003963"
     CDS             <1..213
                     /locus_tag="ACHURJ_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFR1282699.1"
                     /translation="LYDGRTLVQTVTGSLACGVLAMAVAGSLRFFGFPEDAVTFIGAS
                     IGFMGAEKARDKVIAAFNRRVKEKDE"
     gene            210..602
                     /locus_tag="ACHURJ_003964"
     CDS             210..602
                     /locus_tag="ACHURJ_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFR1282700.1"
                     /translation="MSNTFKFSSRSEKNLQGVNPDLVKVTRRALEISEVDFGITEGLR
                     SRYRQKQLVATGKSQTRNSRHLTGHAVDVVAYIGSQVSWEWPLYEKIAAAFRQASREL
                     NIPVEWGGDWKTLKDGPHFQLPHGAYPA"
     gene            599..1006
                     /locus_tag="ACHURJ_003965"
     CDS             599..1006
                     /locus_tag="ACHURJ_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HFR1282701.1"
                     /translation="MKLWPTLGVAFLLIAAWGTSMRLSWSLGRENARNEAQASTLKST
                     VDTLNIISAGVQDMQQVLAQLRVENQQRNQDGEARREQLRNDIAKDECAHALPDARFT
                     DRLRRHAERATASAVSPAYTADADHTGNASPRP"
     gene            1144..1611
                     /locus_tag="ACHURJ_003966"
     CDS             1144..1611
                     /locus_tag="ACHURJ_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1282702.1"
                     /translation="MLKTDSLREAMTRSCRWCQANPEKFTIFVESGNIETTGETPSFV
                     YRYQMVMFVMDYAGELDDLTLPLLAWLSENQPQLLLNPERNQDIKFSAVINDDDSADL
                     LFTLPLRERVRITRNSQGTPQAEHLPEPKPRLPSSEGDWSHVFQDVTWGESNG"
     gene            1604..2239
                     /locus_tag="ACHURJ_003967"
     CDS             1604..2239
                     /locus_tag="ACHURJ_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFR1282703.1"
                     /translation="MDKAFTRVDETFEAIRDSLNQQVINNIARKLAQDLRRAQQARIR
                     SQKAPDGTAWTPRRRRVTRIQERIRFIWNNEARTLKNWHHDTGKYGRTITGWDEDKNN
                     IRTFYRDDIDRFLEIRTRRINQDSTKRVPMFVKLRTARYLKARADASGVTVGYSGVAA
                     RIARVHQFGERDQVAPGIFTDYPVRELLGISQADERLIYNTVLGRIAEAVR"
     gene            2251..2817
                     /locus_tag="ACHURJ_003968"
     CDS             2251..2817
                     /locus_tag="ACHURJ_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFR1282704.1"
                     /translation="MRLLSNIIRTGIISEVDEKSWCVRVRSGELETGWLRWNTTRAGA
                     FNVWLPPSPGEQVVIACIGGNPETAMIIGSLWSDANPAPGKNLKEIVVSAPDGAVFRY
                     DADAGALSASGMKTATLQASVSVTLDTPVVECTNLLRTATLDVTKGGKMSGNITHSGG
                     SFTSNGITVHTHKHGGVKGGSDSTGGPQ"
     gene            2814..3164
                     /locus_tag="ACHURJ_003969"
     CDS             2814..3164
                     /locus_tag="ACHURJ_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFR1282705.1"
                     /translation="MTTRYTGMNPDGTGNLNDMEHLKQSVRDILTTPLASRVMRREYG
                     SLVPDLIDEPMNNTTRLQCMSAAVIALTRWEPRIALDAIDVVWKAGGRAGVTLSGTVM
                     QTMQNVELTITLRE"
     gene            3167..>3681
                     /locus_tag="ACHURJ_003970"
     CDS             3167..>3681
                     /locus_tag="ACHURJ_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HFR1282706.1"
                     /translation="MPAVDLAQLPEPAIIAEPDFEAILADTKAMMIASYPAEQREAVS
                     AALELESEPLNVIAQATAFREMLLRQRINEGARACMLSHSAGTDLDNLAGNMNTKRLV
                     ITPATDTTDAVMESDTSLRLRAQRAYDGLSVAGPSGAYEYFARSASGLVRDARAISPS
                     PANVTVSILST"
BASE COUNT          896 a         1027 c         1071 g          687 t
ORIGIN      
        1 ctttatgacg gacgaacact ggttcagacc gtaacgggat cgctggcgtg cggcgttctt
       61 gccatggccg tggccgggtc gttgcgcttc ttcggttttc ctgaagatgc cgtgacgttt
      121 atcggcgcat caatcggttt tatgggggca gagaaagcac gcgacaaggt tattgcggcc
      181 tttaatcgca gggtgaagga gaaagacgaa tgagcaacac atttaaattc agcagccgga
      241 gcgaaaagaa tttgcagggc gtaaatcctg atctggtgaa agtgacccga cgagcactgg
      301 aaatctcgga agtggatttt ggtatcaccg aagggttgcg cagccgttac cgccagaagc
      361 aacttgtggc cacaggtaag agccagacca ggaacagccg ccaccttacg gggcatgccg
      421 tggatgttgt ggcttatatc ggcagccagg tgtcatggga atggccgctg tacgaaaaaa
      481 tcgcagcagc attcagacag gccagccggg aactgaatat tccggtggaa tggggcggcg
      541 actggaagac cctgaaagac ggaccgcatt ttcagttacc acacggagcc tatccggcat
      601 gaagctctgg cccacgctgg gcgtcgcttt ccttctgatt gccgcatggg gaacatccat
      661 gcgtctgtcg tggtcgctgg gccgggagaa cgccagaaac gaagcgcagg ccagcaccct
      721 gaaaagtacc gtcgacaccc tgaatatcat cagcgccggg gtacaggata tgcagcaggt
      781 gctggcacaa ctccgcgtgg aaaatcagca acgcaatcag gacggagaag ccagacgtga
      841 acagctacgc aacgatattg caaaagatga atgcgcccac gctttgcctg atgctcgttt
      901 tactgacagg ttgcgcaggc acgctgaacg cgccacggcc agcgccgtca gtccggctta
      961 taccgcagac gctgaccata ccggtaacgc ctccccccgt ccctgacact cccacatggg
     1021 gaaatctcgg tatatggggc gaccgccttc tggatgcact ggaaacctgt aacgcggata
     1081 aacgggccat tgaattactg gaacagcgca ggctgcaacg actgaacaac gaggacaaca
     1141 accatgctga aaactgattc cctgcgtgaa gccatgaccc gttcatgccg atggtgtcag
     1201 gccaacccgg aaaaattcac cattttcgtg gagagcggca acattgaaac gaccggagaa
     1261 acgccctcgt ttgtttaccg ctatcagatg gtgatgtttg tcatggatta cgccggggag
     1321 ctggacgacc tcacgctgcc gctactggcg tggttatccg aaaatcagcc acagttgttg
     1381 ctcaatccgg agcgtaatca ggacatcaaa ttttccgccg ttatcaatga cgatgacagc
     1441 gccgatctcc tgtttacgct ccccctgcgg gaacgcgttc gcattacgcg caacagtcag
     1501 ggcacaccgc aggcagaaca cctgccggag ccaaaacccc gtctgccctc ttccgaaggc
     1561 gactggtcgc atgtattcca ggatgtgacg tggggtgaaa gcaatggata aggcattcac
     1621 ccgtgtggat gaaacctttg aggccatccg tgacagcctg aatcagcagg tcatcaataa
     1681 catcgccaga aagctggcac aggatttacg ccgcgcccag caggcacgta tccggtcaca
     1741 gaaagcgccg gacgggaccg cgtggacacc tcgcagacgc cgcgtaaccc ggatacagga
     1801 gcgcattcgc tttatctgga ataacgaagc acgcacgctg aaaaactggc atcacgacac
     1861 ggggaaatac gggcgaacca ttaccgggtg ggatgaggat aaaaacaata tccgcacgtt
     1921 ttaccgggat gacatcgacc gttttctgga aatacgcacc cggcgcatca accaggacag
     1981 cacaaagcgc gtccccatgt tcgtaaaact gcgcaccgcc cgctacctga aagcccgtgc
     2041 agatgcttcc ggtgtgacgg tgggttacag cggcgtggcc gcacgtattg cacgcgttca
     2101 tcagttcggt gagcgcgatc aggttgcgcc gggcattttc accgattacc cggtacgtga
     2161 gctgctgggt atcagtcagg cagatgaacg cctgatttat aacacggtac tgggccggat
     2221 tgcggaggct gtacggtgag cgcagaactc atgcgactgc tgagcaacat catccgtacc
     2281 gggatcatct ctgaagttga tgagaaatcc tggtgcgtgc gcgttcgtag cggcgaactg
     2341 gaaacaggct ggctgcgctg gaacaccacg cgcgcgggag ccttcaacgt gtggcttcca
     2401 ccatcaccag gcgaacaggt ggtaattgcc tgcatcggcg gcaaccctga aaccgccatg
     2461 ataattggca gtctgtggag tgatgccaat ccggcacccg gcaaaaacct gaaagaaatc
     2521 gtggtcagcg cgccggatgg cgcggtgttc cgctacgacg cagacgctgg cgcactgagc
     2581 gccagcggca tgaaaacagc caccctgcag gcatccgtca gcgtgacact ggatacgccc
     2641 gtcgtggaat gcacaaacct tctgagaaca gcgacgcttg acgtcacaaa aggaggaaag
     2701 atgagcggca atatcacgca cagcggcggc agcttcacct caaacggcat cacagtgcat
     2761 acgcataaac acggcggcgt taaaggtggc agcgattcga caggaggccc gcagtgacaa
     2821 cccgctacac aggaatgaat ccggacggga cgggaaacct gaacgatatg gagcacctga
     2881 aacagtcagt cagggacatc ctgaccaccc cgctggcaag ccgggttatg cgacgggaat
     2941 atggcagcct tgtgcctgat ttgattgacg aacccatgaa taacaccacg cgtctgcaat
     3001 gcatgagtgc tgccgtgatt gcgctgacac gatgggaacc ccgcattgcc ctggatgcca
     3061 tcgacgttgt ctggaaagcg ggaggccgcg ccggggtgac gttgtcgggc actgtcatgc
     3121 agaccatgca aaatgttgaa ttaaccatca cgctgaggga gtaatcatgc ctgccgttga
     3181 ccttgcccag ttaccggaac ccgccatcat cgcggagcct gattttgagg caattctggc
     3241 tgacacaaag gccatgatga ttgcgtccta tcctgctgaa cagcgtgaag ccgtctccgc
     3301 cgcgctggag ctggaatcgg aacccctgaa cgttatcgcc caggcaacag cgtttcgtga
     3361 aatgctgtta cgccagcgga tcaatgaggg tgcacgcgcc tgcatgctaa gccacagcgc
     3421 cgggacagac ctggacaacc tcgcgggcaa tatgaacaca aagcgcctgg ttatcactcc
     3481 ggcaacggat accaccgacg cagtgatgga aagtgacacc tcgctgagac tgcgggcgca
     3541 acgggcgtat gacggtctga gtgttgctgg cccgtcaggt gcatacgagt attttgcacg
     3601 cagcgccagc ggtctggtac gcgacgcgcg ggccatcagt ccgtctccgg caaatgtgac
     3661 ggtttccatc ctgtccactg a
//
LOCUS       DAZPEB010000267         3679 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZPEB010000267 DAZPEB010000000
VERSION     DAZPEB010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.272"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(168..482)
                     /gene="fliE"
                     /locus_tag="ACHURJ_003971"
     CDS             complement(168..482)
                     /gene="fliE"
                     /locus_tag="ACHURJ_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFR1282707.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQDSLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            697..2355
                     /gene="fliF"
                     /locus_tag="ACHURJ_003972"
     CDS             697..2355
                     /gene="fliF"
                     /locus_tag="ACHURJ_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFR1282708.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     LSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTSRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            2348..3343
                     /gene="fliG"
                     /locus_tag="ACHURJ_003973"
     CDS             2348..3343
                     /gene="fliG"
                     /locus_tag="ACHURJ_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFR1282709.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            3336..>3679
                     /gene="fliH"
                     /locus_tag="ACHURJ_003974"
     CDS             3336..>3679
                     /gene="fliH"
                     /locus_tag="ACHURJ_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="binds to and inhibits the function of flagella
                     specific ATPase FliI; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFR1282710.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAKAKSQQAPIHARMQQLV
                     SEFQTTLDALDSV"
BASE COUNT          925 a          967 c         1029 g          758 t
ORIGIN      
        1 cttcctatga gcaaagcgaa gacaaaacat gttatcgcgt ttacggatcg tggatggcag
       61 aaaactttca ggatcaggta tccattttaa accagaaata gggtgagttt gtcccatcca
      121 tgccccacgc gggcagatgg gatgtaatta ccaacaggtt acaaaaccta cacctgcatg
      181 gacatcactt cctgatacgc cgccaccagc ttattacgca cctgaatccc catttgcata
      241 gaaactgagg ctttttgcat atcggtcatc acatcgttta acgccacgcc gggttcaccg
      301 agggtgaatt tttctgcctg cgtgcgggcg gctgtttgtg tatcgcttat gcgatcgagc
      361 gcggcgtgca gctgcccggc aaaactaatg gtcggttgcg gcagtgaatc ctgcgcacgc
      421 gcactcatcg ccgtagcctg taactggctg ataacccctt caatcccctg tatcgctgac
      481 attttcgtct cctggataat ttctggtagc aaagcctacc agtaagtcaa taagacaaag
      541 gcgctaaata gcaacaaaaa aacgggttta ttggcggata gaaaaaaacg aaagcacaaa
      601 taatggcagc gtcaattttt cgagtttgct gacccgggag tgagtcttgt tccactttgc
      661 caataacgcc gtccataatc agccacgagg tgcgcgatga atgcgactgc agcccagaca
      721 aaatctcttg agtggcttaa tcgcctgcgt gcgaatccga aaattccatt gattgttgcc
      781 ggttccgcgg cagtggcggt catggtcgca ctgatcctgt gggcgaaagc ccccgactac
      841 cgcacattat tcagcaatct ttccgatcag gatggtggcg caattgtcag ccaactgacg
      901 caaatgaata ttccttaccg cttcagcgaa gccagcggcg ctattgaagt tccggcagat
      961 aaagttcacg agctgcgtct gcgcctggcg caacaaggtt tgcccaaagg cggcgcggtc
     1021 ggtttcgaac tactcgatca ggaaaagttt ggtatcagcc agttcagcga acaggtgaat
     1081 tatcagcggg cgctggaagg cgagctttct cgtaccatcg aaactatcgg cccggtaaaa
     1141 ggggcgcgcg tacatctggc aatgccgaaa ccgtctttat tcgtccgtga acaaaaatcc
     1201 ccttctgcat cggtgacggt aaatctatta cccggccgcg cactcgatga agggcaaatt
     1261 agcgccattg tacatctggt ttccagcgcg gttgctggtc tgccgccggg gaacgtcacg
     1321 ctggtggatc agggtggaca tctgttaacc ctgtccaata ccagcgggcg cgatcttaat
     1381 gacgctcagt tgaaatatgc cagcgatgtc gaaggccgga ttcagcggcg tattgaagcg
     1441 atcctgtcgc caattgttgg taacggtaat attcacgccc aggtcacggc gcaactggac
     1501 ttcgccagta aagaacaaac ggaagaacag tatcgcccta acggtgatga atctcatgcg
     1561 gcgcttcgtt cacgccagct taatgagagc gagcaaagcg gttccggtta tccgggcggc
     1621 gtaccggggg cgttgtcgaa tcaaccggca cctgcgaata acgcgccaat cagcacgcct
     1681 ccggcaaatc aaaataaccg ccagcagcag gcgagcacca ccagcaatag cgggccgcgt
     1741 agcacacagc ggaatgaaac cagtaactac gaagtcgatc gcaccattcg tcataccaaa
     1801 atgaacgtgg gcgatgtgca acgtctgtca gtcgcggtag tggtaaatta caaaaccttg
     1861 ccagacggta aaccattgcc tctcagcaac gaacagatga agcaaattga agatctgacc
     1921 cgcgaggcga tgggcttttc tgaaaaacgc ggtgactcgc tcaatgtcgt taactcgccg
     1981 ttcaatagca gtgacgaaag cggcggagaa ctgccgttct ggcaacagca agcgtttatc
     2041 gatcagttgc tggctgccgg tcgctggttg ctggtactgc tggtggcgtg gctgctgtgg
     2101 cggaaagcgg tacgcccgca gttaacaagt cgcgccgagg cgatgaaagc tgtacagcaa
     2161 caggcgcagg cccgtgagga agtggaagat gcggtggaag tccgcctgag caaagacgaa
     2221 caactccaac aacgacgcgc taaccaacgt ctgggggcag aagtcatgag ccagcgtatc
     2281 cgtgaaatgt ctgataacga tccgcgcgtg gtggcgctgg tcattcgcca gtggataaat
     2341 aacgatcatg agtaacctga caggcaccga taaaagcgtc atcctgctga tgaccattgg
     2401 cgaagaccgg gcggcagagg tgttcaagca cctctcccag cgcgaagtgc aaaccctgag
     2461 cgctgcaatg gcgaacgtca cgcagatctc caacaagcag ctaaccgatg tgctggcgga
     2521 gtttgagcaa gaagctgaac agtttgccgc actgaatatc aacgccaacg attatctgcg
     2581 ttcggtattg gtcaaagctc tgggtgagga acgtgccgcc agcctgctgg aagatattct
     2641 cgaaactcgc gataccgcca gcggtattga aacgctcaac tttatggagc cgcagagtgc
     2701 cgccgatctg attcgcgatg agcatccgca aattatcgcc accattctgg tgcatctgaa
     2761 gcgcgcccag gccgccgata ttctggcgtt gttcgatgaa cgtctgcgcc acgacgtgat
     2821 gttgcgtatc gccacctttg gcggcgtgca gccagccgcg ctggcggaac tgaccgaagt
     2881 actgaatggc ttgctcgacg gtcagaatct caagcgcagc aaaatgggcg gcgtgagaac
     2941 ggcagccgaa attatcaacc tgatgaaaac tcagcaggaa gaagccgtta ttaccgccgt
     3001 gcgcgaattc gacggcgagc tggcgcaaaa aattatcgac gagatgttcc tgttcgaaaa
     3061 tctggtggat gtcgacgatc gcagcattca gcgtctgttg caggaagtgg attccgaatc
     3121 gctgttgatc gcgctgaaag gggccgagca gccactgcgc gagaaattcc tgcgcaatat
     3181 gtcgcagcgt gccgccgata ttctgcgcga cgatctcgcc aaccgtggtc cggtgcgtct
     3241 gtcgcaggtg gaaaacgaac agaaagcgat tctgctgatt gtgcgccgcc ttgccgaaac
     3301 tggcgagatg gtaattggca gcggcgagga tacctatgtc tgataatctg ccgtggaaaa
     3361 cctggacgcc ggacgatctc gcgccaccac aggcagagtt tgtgcccatg gtcgagccgg
     3421 aagaaaccat cattgaagag gccgaaccca gccttgagca gcaactggcg caactgcaaa
     3481 tgcaggccca tgagcaaggt tatcaggcgg ggattgccga aggtcgccag caaggtcatg
     3541 agcagggcta tcaggaagga ctggcccagg ggctggagca aggtctggca aaggcgaagt
     3601 ctcaacaagc gccaattcat gcccggatgc agcaactggt cagcgaattt caaactaccc
     3661 ttgatgcgct tgatagtgt
//
LOCUS       DAZPEB010000268         3640 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZPEB010000268 DAZPEB010000000
VERSION     DAZPEB010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3640
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.273"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..71
                     /locus_tag="ACHURJ_003975"
     CDS             <1..71
                     /locus_tag="ACHURJ_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282711.1"
                     /translation="VTAVETNVLRLHGRLSREYGSK"
     gene            331..423
                     /locus_tag="ACHURJ_003976"
                     /pseudo
     CDS             331..423
                     /locus_tag="ACHURJ_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004120448.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            427..606
                     /locus_tag="ACHURJ_003977"
                     /pseudo
     CDS             427..606
                     /locus_tag="ACHURJ_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            940..3219
                     /gene="ydeP"
                     /locus_tag="ACHURJ_003978"
     CDS             940..3219
                     /gene="ydeP"
                     /locus_tag="ACHURJ_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFR1282712.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHTASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIEHISGLSQTQIAELADAYAAADRTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3467..>3640
                     /gene="safA"
                     /locus_tag="ACHURJ_003979"
     CDS             3467..>3640
                     /gene="safA"
                     /locus_tag="ACHURJ_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFR1282713.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHF"
BASE COUNT          979 a          813 c          880 g          968 t
ORIGIN      
        1 tggtgacggc agttgaaacc aatgtgttga ggttgcatgg acggttaagc cgagaatacg
       61 gtagtaagtg agatatgggg tttggtcgtt gttaatgagc aaccatgacc gccaacgact
      121 ggattctccc ttggtttatg ttggtgcgca taatgtatat tatgttaaat catatctgag
      181 tgatgcgaag tgcttttctg gtagtcgtta ttcgttcagg gttccagaac ttaagcctct
      241 cctctttcat aattggcctg tgcatgacgg atataggctg gctggtatgc agatttgccg
      301 tttgccgcat cttcccagcg ttgaagactc aagctggcaa accgcgtgca aatgagcacg
      361 ccctctttcc atcaacactt caggcagctt gcgggaatga gtccactacg gtatcagaaa
      421 tgggaagtgg gtgtctctct gatgcgtaat ggaaaaatac tggctacggg agaaaatgtc
      481 tccctgggga cagtaaataa aagcaaagta tctctgggtt taagcgcaac ctatggacaa
      541 acaggaaaca aggttgcagc cggaaccgtc cagtcagtca ttggtgtcac ttttatctat
      601 gaataattgt gattttgatc ttagtctatg cgctccattc acgaaaattg cgcggataat
      661 tattttgtga aggctattat cctacacctg taagaaaatc cgcgcacaat tgaagccaga
      721 gtcttatttt ttatattgaa aaatatgtta atttaaacac tatgagagat gatgcttaac
      781 ttatcgtatt taatctattg tttaacgata atagattaaa aatagaaaga gagtagtccc
      841 ctactcttat atatccatgt tggcgataat ccctttgtat gtactgtgca tcatcgctat
      901 tacaaatcct aataattcat ttccacacag gataagtaga tgaagaaaaa aattgaatcc
      961 taccagggtg ctgcaggtgg ttggggtgct gttaaatccg tagcgaatgc agtacgtaag
     1021 cagatggata tacgccagga tgttattgcc atgtttgaca tgaataagcc agagggcttt
     1081 gactgtccgg gttgtgcatg gccagatcct aagcacactg cgtcattcga catttgtgaa
     1141 aacggcgcaa aagcaatcgc ctgggaagtc acggataagc aggtaaatgc ctctttcttt
     1201 gctgagaata cggttcaatc attacttacc tggggagatc acgagcttga ggctgcggga
     1261 cgtctcactc agcctttgaa atatgatgcc gtcagcgact gttacaagcc attaagctgg
     1321 caacaagctt tcgacgaaat tggcgcacgc cttcaaagct atagtgatcc caatcaggtt
     1381 gaattctata cttcgggccg cacttccaat gaagctgcct ttctttatca gctttttgcc
     1441 cgtgaatacg ggagcaataa ctttcccgac tgctccaaca tgtgccatga accgaccagt
     1501 gtgggtttgg cagcgagtat cggcgtgggt aaagggactg tgttgctgga agactttgag
     1561 aagtgcgatt tagtcatttg cattgggcat aaccctggta caaaccaccc ccgcatgctg
     1621 acttcgttgc gcgctttagt gaaacgggga gcgaaaatga tcgccatcaa tcctttacag
     1681 gaacgtggcc tggagcgatt taccgcaccg caaaacccgt ttgaaatgct gacgaactct
     1741 gagactcagt tggccagtgc gtactacaac gtgcgcattg gtggcgatat ggcgttgctc
     1801 aaggggatga tgcgcctgtt aattgagcgc gatgatgctg caagtgccgc aggtcggccc
     1861 tcattgcttg atgacgaatt tattcaaacg cataccgtcg gctttgacga gctacgccgt
     1921 gacgttctca attccgagtg gaaagatatc gaacatattt ctggactaag tcagacacaa
     1981 atcgccgaac tggctgacgc atatgccgct gccgatcgca ccattatctg ttacggaatg
     2041 gggatcactc agcacgaaca tggtacccag aatgtacagc aactggtcaa tctgctgttg
     2101 atgaaaggta acattggcaa gcctggtgcg ggtatctgcc cactacgagg tcactctaat
     2161 gtacagggcg accgaaccgt cggtatcacc gagaaaccgt ctgcagagtt tctggctcgc
     2221 ctgggtgagc gctatggctt caccccacct catgcacctg gacatgctgc aattgccagc
     2281 atgcaagcaa tatgcacggg gcaggctcga gcattgatct gcatgggggg caactttgca
     2341 ctggcaatgc cagatcggga agcgagcgct gtaccgttaa cgcaattaga tttggcggta
     2401 cacgtagcca ctaagcttaa ccgctctcat ctgctgaccg cacggcatag ctatattctg
     2461 ccggttctgg ggcgtagcga gattgacatg caaaaaagcg gtgcgcaggc ggtaaccgtt
     2521 gaggattcaa tgtcgatgat tcatgcctcg cgtggcgtgt taaaacccgc cggtgtaatg
     2581 ctgaaatcag agtgtgcagt ggtcgcggga atcgcgcagg cagcactacc ccagagcgtg
     2641 gtagcctggg agtatctggt ggaagattat gatcgcattc gcaatgacat tgaagctgtg
     2701 ttaccagagt tcgccgacta taaccagcgc atccgacatc ctggaggttt tcacctgata
     2761 aatgcagctg ctgaaaggcg ctggatgacg ccgtcaggta aggctaattt cattaccagc
     2821 aaagggctgt tagaagatcc ctcttcagcg tttaacagta agctggtcat ggcgacagta
     2881 cgcagccacg atcagtacaa cacgacgatt tatggtatgg atgatcgcta tcgaggggta
     2941 ttcggtcaac gagatgtggt ctttatgagt gctaaacaag ctaaaatttg ccgtgtaaaa
     3001 aacggcgaaa gagttaatct tattgcgctt acgccagacg gtaagcgcag ctcacgccgc
     3061 atggatagat taaaagtggt catttaccct atggctgacc gctcactggt gacctatttt
     3121 ccagaatcga atcacatgct aactcttgat aaccacgatc cattaagtgg cattcctggt
     3181 tataaaagta ttccggttga attagaacca tcaaattaat tcgcctcctc atttcttctg
     3241 ctgtcatccg cacagcagaa gaatttctca ttgactatta tcgcgcaatt tgctcacatt
     3301 gatcaaatta aaccacacac tataagatat aaacctctgc ctacagctgt aagaaactcc
     3361 gctcagtact gaagtgccag tcctatttcc tcttttctcc agcctgttat attaagcata
     3421 ctgattaaca atttttaacg ttatccgcta aataaacata tttgaaatgc atgcgaccac
     3481 agtgaaaaac aaaatcacgc aaagagacaa ctataaagaa atcatgtctg taattgtggt
     3541 tgtcttatta ctgaccctta cgttgatagc cattttttcg gcaattgatc agctgggtat
     3601 ttcagaaatg ggtcgcattg caagagatct tacacatttc
//
LOCUS       DAZPEB010000269         3546 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZPEB010000269 DAZPEB010000000
VERSION     DAZPEB010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.274"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            1..727
                     /locus_tag="ACHURJ_003980"
                     /pseudo
     CDS             1..727
                     /locus_tag="ACHURJ_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105555.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(755..844)
                     /locus_tag="ACHURJ_003981"
     tRNA            complement(755..844)
                     /locus_tag="ACHURJ_003981"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(808..810),aa:Ser,seq:cga)
     gene            938..1735
                     /gene="mtfA"
                     /locus_tag="ACHURJ_003982"
     CDS             938..1735
                     /gene="mtfA"
                     /locus_tag="ACHURJ_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFR1282714.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            1836..1911
                     /locus_tag="ACHURJ_003983"
     tRNA            1836..1911
                     /locus_tag="ACHURJ_003983"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1869..1871,aa:Asn,seq:gtt)
     gene            2225..3546
                     /locus_tag="ACHURJ_003984"
                     /pseudo
     CDS             2225..3546
                     /locus_tag="ACHURJ_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta domain-containing
                     protein"
BASE COUNT          963 a          827 c          850 g          906 t
ORIGIN      
        1 gtgccggcag attccataaa accagaacac atccgtcgat acatggacaa gagaggggaa
       61 cagagtaaaa cgcaagccaa ccatgaagtc ccgcgtttac agttgggggt atgagcgagg
      121 gtacgtgaag gctaacccat gtgcaggtgt aagtaaattc aaggccaaaa accgcgaacg
      181 atatgtaacc gacaaagaat accaggcagt attaagcgtt gcacctcttc ctgtttttat
      241 cgcaatggaa attgcctatc tgtgtgcagc gagggtttcc gatgtgttat cgctgaaatg
      301 ggagcagatt ggaaacgacg ggatctttat ccagcaaggg aaaacaggaa aaaaacagat
      361 aaaagcatgg agtccacgat tacaggcggc gatcgaaaaa gcaaaacagt taccaacatc
      421 cgcctatgta atcagcaatc aatacggcaa ccgatatatg tacaaaggct ttaacgaaat
      481 gtgggtagaa gcaagaaatc gcgcaggcaa aatttcaggt attttaaccg acttcacctt
      541 tcatgatctg aaggcgaaag gaatttcaga ctatgaagga agcagtcggg ataagcaact
      601 tttctctggt cacaaaaccg aggggcaagt gctaatctat gacaggaagg ttaaagtttc
      661 accgacactt gatgtcccgt tacctgaaaa tattccaagt aattccacgt gtgatttttg
      721 tcactgactt aatgatgtat aagtgattga attttggcgg agagaggggg atttgaaccc
      781 ccggtggagt tgcccccact ccggttttcg agaccggtcc gttcagccgc tccggcatct
      841 ctccgttcag atggttgcca tgatgccagg aaatttggca ttttaacagt ccctgtccgt
      901 gcaattttgt tcaagtgacg agtttgcgag caaaacgatg attaagtggc cctggaaagt
      961 acaagaatca gcacatcaaa ctgcccttcc ctggcaggaa gcactatcga tccccctttt
     1021 aacgtgtctg acggaacagg aacaaagcaa attagtcact cttgccgaac gttttttaca
     1081 gcaaaagcgg cttgttcctt tacagggctt tgaactggat tcattaagaa gctgccggat
     1141 agcacttcta ttttgcctac ccgttctgga gttaggactg gaatggctgg atggttttca
     1201 tgaagtctta atttatcctg cgccatttgt ggtcgatgat gaatgggaag acgatatcgg
     1261 tctggtgcat aaccaacgta ttgttcagtc aggtcagagc tggcagcaag ggcctatcgt
     1321 tttgaactgg ttggatatac aagattcttt tgatgcttct ggttttaacc tgattattca
     1381 tgaagtcgct cataagctgg acacccgtaa cggcgatcgc gccagcggag ttccctttat
     1441 tccgttgcgt gaggttgctg gctgggaaca cgatcttcat gctgcaatga acaacattca
     1501 ggaagaaatc gaattggttg gcgagaatgc ggcgagcatt gatgcttatg cagccagcga
     1561 tcctgctgaa tgttttgccg tactttctga atatttcttt agcgccccag aactttttgc
     1621 tcctcgtttc ccttcattgt ggcaacgttt ctgccaattt tatcaacaag atcctttgca
     1681 gagactgcat cacgctaatg atacagactc gttttcggcg acgaatgttc attaattaac
     1741 aactttgcat attaattaac caattgaaat gacttatgaa atttagtgtt gacagacaag
     1801 gtaccgctaa gtaatatgcg ccccgttcac acgattcctc tgtagttcag tcggtagaac
     1861 ggcggactgt taatccgtat gtcactggtt cgagtccagt cagaggagcc aaattctaaa
     1921 aattcgcttt tttagcgcaa tgtcactgac cttagttgaa cattgttttt taacggatag
     1981 cgggttttta acatcttaag cgccctcgac ctttatggtt gagggcgttt tgctatgaac
     2041 gccatcacca ttttcccctc gattataaaa cttgagttat tcagtagtct cccctcttgc
     2101 aactcacacc caaaactgcc taacgaaaag ttattaattt tcaatcatat tgctatcagt
     2161 atttacattt tttcgctgtg ctagaaaggg cgcatttatg ttagctcgtt cagggaaggt
     2221 aagcatggct acgaagaaga gaagtggaga agaaataaat gaccgacaaa tattatgcgg
     2281 gatgggaatt aaactacgcc gcttaactgc gggtatctgt ctgataactc aacttgcgtt
     2341 ccctatggct gcggcagcac aaggtgtggt aaacgccgca acccaacaac cagttcctgc
     2401 acaaattgcc attgcaaatg ccaatacggt gccctacatc cttggagcgc tggaatcggc
     2461 ccaaagcgtt gccgaacgtt tcggtatttc ggtggctgag ttacgcaaac tcaaccagtt
     2521 tcgtacgttt gctcgaggtt ttaataatgt ccgccagggt gatgaactgg atgtcccggc
     2581 acaagttagt gaaaaaaaat taaccccgcc gccgggtaat agcagtgaca acctcgagca
     2641 acagatagcc agtacttcac agcaaatcgg gtctctgctc gccgaagata tgaacagcga
     2701 gcaagcggca aatatggcgc gtggatgggc ctcttctcag gcttcaggcg caatgacaga
     2761 ctggttaagc cgcttcggta ccgcaagaat cacgctgggc gtggatgaag attttagcct
     2821 gaagaactcc cagttcgatt ttctccatcc gtggtatgaa acgcctgata atctcttttt
     2881 cagtcagcat actctccatc gtactgacga gcgtacgcag attaacaacg gcttaggttg
     2941 gcgtcatttc actcccacat ggatgtcagg catcaacttc tttttcgacc acgatcttag
     3001 ccgttaccac tcccgcgccg gcattggcgc ggagtactgg cgcgactatc taaaattaag
     3061 cagtaacggc tatttgcgac tgaccaactg gcgcagcgca cctgaactgg acaacgatta
     3121 tgaagcacgc ccggccaatg gctgggatgt acgcgcagaa agctggctac ccgcctggcc
     3181 gcaccttggc ggtaaactgg tctatgaaca gtattatggc gatgaagtgg ccctgttcga
     3241 taaagacgat cggcaaagta atcctcatgc cataaccgct ggacttaact ataccccctt
     3301 cccgctgatg accttcagcg cggagcaacg ctagggtaaa cagggcgaaa atgacacccg
     3361 ttttgccgtc gattttacct ggcaacctgg cagcgcaatg cagaaacagc ttgacccgaa
     3421 tgaagtcgct gcacggcgta gccttgcagg cagccgttat gatctggtgg atcgcaacaa
     3481 caatatcgtt ctggaatatc gcaaaaaaga actggttcgc ctgaccctga cagaccccgt
     3541 gacagg
//
LOCUS       DAZPEB010000270         3510 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZPEB010000270 DAZPEB010000000
VERSION     DAZPEB010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.275"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(104..391)
                     /locus_tag="ACHURJ_003985"
     CDS             complement(104..391)
                     /locus_tag="ACHURJ_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFR1282715.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            complement(466..609)
                     /gene="mgrB"
                     /locus_tag="ACHURJ_003986"
     CDS             complement(466..609)
                     /gene="mgrB"
                     /locus_tag="ACHURJ_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFR1282716.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            768..1007
                     /locus_tag="ACHURJ_003987"
     CDS             768..1007
                     /locus_tag="ACHURJ_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFR1282717.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            complement(1150..1941)
                     /gene="kdgR"
                     /locus_tag="ACHURJ_003988"
     CDS             complement(1150..1941)
                     /gene="kdgR"
                     /locus_tag="ACHURJ_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFR1282718.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            2118..3491
                     /locus_tag="ACHURJ_003989"
     CDS             2118..3491
                     /locus_tag="ACHURJ_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1282719.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVMLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRSRA"
BASE COUNT          844 a          880 c          793 g          993 t
ORIGIN      
        1 agaccagagt tttaatttga agcagttgca ccatgacagt gcgtataatc aggatgaatt
       61 gagtaaccct tcaacgacgg ggttactcaa gattgaggaa ggattatcgt tcggcgacca
      121 ggcgaataat atcttcgtca tcaaccgatt tttgactctc aatggtcttt ttatctgctt
      181 tctctggttc gtttgcttcg cacaaacgac gtaacagtgc attttgacgt ttttgctgat
      241 ccagcaacgc ctcaagcagt tcaatctgtt cgttagtccg tgaactggca cgattgataa
      301 aaaaccacaa gatgagcccg ataagaagaa ctaccaccga tacaaccaaa gacgcaatgt
      361 tcatcacgcc tgaatttaca acttcgttca tttcaccacc tctgagtaga ggcgctattc
      421 taccactgct ggagaggaag aaaatctagt gctgaaaaaa tgatatcacc acgggataaa
      481 ctggttaatg gcacaaattc cgctgaaaaa ttgtacatcc tgatcgcaca tcatgttgaa
      541 tacctgcgcc caaagcagca agcaagccaa caccacgaca accagaacga cccatcgaaa
      601 ctttttcact ccaatctccg cttcacctac cttatgtcat taaactagca tgcgttagtt
      661 aaacagcgcc taactatgtc gatattcgtg ctttttcgga atgagtagct tgcttcagcg
      721 catattgccg ctacgattaa gcgaacataa aaagagaaga ggttgtaatg cgattcatca
      781 ttcgcacagt tatgctgata gcactggtat ggataggatt attactcagc ggatatggcg
      841 ttttgattgg aagcaaagag aatgccgcag gattaggttt gcaatgtacc tatctgactg
      901 ccagaggcac cagcacggta caatacttgc acactaaaag tggtttcttg gggataacag
      961 attgcccact gttacgtaaa agcaatatcg tggtcgataa tggttaaggg taataaataa
     1021 ccagattctc actcatgagt aaaatattct tatagcaagc gcgtttaata taacgcacct
     1081 tcaactttat ttttctcatg ctaaataaag aacctgtctg tttgatattc cgacaggttc
     1141 ttactctcat cagaacggat agtcgtgata acccatttgg gcagaaattt tgcgcgctgc
     1201 ggtatgcaac attgcgacat attcctgtaa acgctcttca gagaaacgca acgtcgggaa
     1261 ggagatgctc aaaccggcaa tgaccacgcc aaagcgatca aataccggta ccgcaatgca
     1321 tcgcagccct tcttcctgct cttcgttatc ttcgccatac ccctgctcgc gcacctggtc
     1381 cagaacgggt aataacgctt ctgtactggt gatggtccgc tcggtactgc gtttatactc
     1441 cacgccctca agaatttgct tcacttcatc gcgatcgcgc catgccagca gtaccttacc
     1501 aatcgcggtg ctgtacagcg gattacgacg cccaatccgt gaatacatgc gcaaattgta
     1561 catagagtca attttgtgaa tgtaaacaat actgtcttcg tccagtgcgc cgaggtggat
     1621 agtttctttg gtcaggcggg agatctcacg catctggata tctgcgctac gaattaaatc
     1681 gacgttttgt aacgcgcgag cgcccagttc aaacaatttc agggtcaggg aatatttctc
     1741 cgactcccct tcctgcgcca cataacctaa ggttttcatg gtctgtaaaa agcgataaac
     1801 ggtgcttttt gacatcatga cgcgctgcga cagctcggtt atccctattt cgcgctcttc
     1861 acccagcgcc tgcaaaatgc caaaaacttt tagcacggaa gatacagaat caggctgttt
     1921 atccagatct gcgttagcca tttatcacct cattgcgagt gttttataaa aatcagaact
     1981 gttttttatt ataatttcgc accagggggg tcgcaatcca tcttttgccg gttagttaca
     2041 attctgcgac atccaccgtg aatatcagtg ctagaatcat acccctgttg attattcacc
     2101 aaagatataa aattcctatg ccaaaagttc aggccgacgg cctgccattg ccccagcgat
     2161 acggtgcgat attaaccatt gtgattggta tttcgatggc tgtccttgac ggcgcaatcg
     2221 ccaacgtcgc cctgccaaca atcgccacgg accttcatgc cacgccagcc agttccatct
     2281 gggtagtgaa cgcctatcaa atcgccattg tcatctccct gctctcgttt tcgtttctgg
     2341 gcgatatgtt tggctatcga cgtatttata aatgcggtct ggtcgttttt ctgttgtctt
     2401 cactgttctg cgccctttct gattcgctgc aaatgctcac ccttgcgcgt gtcatacaag
     2461 gtttcggcgg tgcagcgttg atgagcgtta ataccgcact tatccgcctg atctatccac
     2521 aacgttttct ggggagaggg atgggcataa actcgtttat tgttgccgtc tcttctgctg
     2581 ccgggccgac aattgctgca gcaatcctct ccatcgcatc ctggaaatgg ttatttttaa
     2641 tcaacgtacc gttaggtatt atcgccctgc ttctggcgat gcgttttctg ccacccaatg
     2701 gttctcgcgc cagtaaaccc cgtttcgacc tgcccagcgc cgtgatgaac gcgttaacct
     2761 tcggcctgct tatcactgcg ttgagtggtt tcgctcaggg gcaatcgctg acgttaattg
     2821 ctgcggaact ggtggtaatg gttgttgttg gtattttctt tattcgccgc cagctttctc
     2881 ttcccgtacc gctgctaccg gtggatttac tgcgtatccc gctgttttca ctttctattt
     2941 gcacatctgt ttgctctttc tgcgcacaaa tgctggcaat ggtttccctg cccttttacc
     3001 tgcaaaccgt gctcgggcgt agtgaagtcg aaacaggttt acttctgaca ccgtggccgt
     3061 tagcaacgat ggtgatggct ccgttggcag gctatttgat tgaacgcgta catgcaggat
     3121 tgctgggtgc tctagggttg ttcatcatgg ctgcggggct tttttccctg gttatgctgc
     3181 ccgcgtcacc tgcggatatc aatattatct ggccgatgat cttatgtggc gctggatttg
     3241 gcttgttcca gtcacccaat aaccacacca ttattacctc cgctcctcgc gaacgtagcg
     3301 gtggagccag tggcatgtta ggaacggctc gtctactggg tcagagtagc ggcgcggcac
     3361 tggtggcgct gatgctaaat cagttcggtg ataatggtac gcacgtttcg ctgatggctg
     3421 cggctattct ggcagtgatt gctgcctgtg tcagtggttt acgtatcact cagccacgat
     3481 ccagggcata aaaaaagcgc gtcgatcagg
//
LOCUS       DAZPEB010000271         3496 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZPEB010000271 DAZPEB010000000
VERSION     DAZPEB010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3496
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.276"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            33..218
                     /gene="ymgJ"
                     /locus_tag="ACHURJ_003990"
     CDS             33..218
                     /gene="ymgJ"
                     /locus_tag="ACHURJ_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HFR1282720.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TAFKKCLFLMRDIIISS"
     gene            179..298
                     /locus_tag="ACHURJ_003991"
     CDS             179..298
                     /locus_tag="ACHURJ_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFR1282721.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(777..1043)
                     /gene="minE"
                     /locus_tag="ACHURJ_003992"
     CDS             complement(777..1043)
                     /gene="minE"
                     /locus_tag="ACHURJ_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFR1282722.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(1047..1859)
                     /gene="minD"
                     /locus_tag="ACHURJ_003993"
     CDS             complement(1047..1859)
                     /gene="minD"
                     /locus_tag="ACHURJ_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFR1282723.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(1883..2578)
                     /gene="minC"
                     /locus_tag="ACHURJ_003994"
     CDS             complement(1883..2578)
                     /gene="minC"
                     /locus_tag="ACHURJ_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFR1282724.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            3104..3472
                     /locus_tag="ACHURJ_003995"
     CDS             3104..3472
                     /locus_tag="ACHURJ_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFR1282725.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQITTKMLFMCRE"
BASE COUNT          897 a          806 c          794 g          999 t
ORIGIN      
        1 ctgatttatc aatattattg tgtgattttt atgtgagcag aagatattca tcagcaacga
       61 ttacattagt cattttattt tgccgacggc ctcattgtcg aaagataagc gtacgacagt
      121 attatctgaa aagagtgatt ttttatccaa ctacacttca gcgcactgcg tttaaaaaat
      181 gcctctttct tatgcgggat atcatcattt catcatgatg tctttggtga gtggtgaaca
      241 caatacacct gcgctgtctc ttcaggatga atcccctggt ctggtgcctg cgggctgatg
      301 ttgcagcaga gcttaggtca cttagacgct actatcattt atccaatggc atggaatcga
      361 aatcagtcga tacccgcagt atatatcgtg aactggtcgt tctgaccatc aacgattaaa
      421 ctgcgcttcg gcgcagtttt cgtttacagg gggctaaaag ggaaaattct ggggcaaaaa
      481 aagcccgcca gttacggcgg gaaacctcat cctatgggag aacaatgaat aatgaaattg
      541 cggggttatc atcccccagt gtatccacac taacaatgag gttatttact caaccaggca
      601 taaacatttt gttctgtgcg tgggaacagc cttaaggtgt aaagggggag gtggaaatag
      661 caatgaggag tatcagcaag aatactcgcc gctttaccac aacgtggatg agagggatga
      721 aaaactcaag gcagaaatga ctctgccttg aagataaatg cgtttttaca gcgggcttat
      781 ttcagctctt ctgcttccgg taaggtcacg ttcagctcaa gaatagaaat atcgccatct
      841 ttttgctcaa gctgtacggt taccatctca ggatcaattt gtacgtattt acaaatgacc
      901 tcaagaatat ctttacgcaa ctgcggcaga taatgcggtt ctgcatcgct gcgacggcgt
      961 tcagcaacaa taatctgcag ccgttctttt gcaatgttgg ctgtgttttt cttccgcgag
     1021 agaaagaaat cgagtaatgc cataacttat cctccgaaca agcgtttgag gaagcctttc
     1081 ttctcttctt caatgaagcg gaaaggacgt tcttctccca acagacgttc tacggtatct
     1141 gcgtaggctt tacccgcatc ggcgttaatg tcgagaatga ccggttcacc ctggttagag
     1201 gcgcgcaaca ctgattgatc ctctgggatc acgccgacga gtttgatgcg caggatctcc
     1261 agcacatctt ccatgctcag catgtcacct ctgcttacgc ggcctgggtt atagcgcgtt
     1321 aacagcaggt gctctttaat aggctcttcg ccattttctg cgcggcgtga tttcgacgcc
     1381 agaatgccta aaatacggtc agaatcgcgt actgaggaga cttccgggtt ggtggtaata
     1441 atggcttcgt ctgcaaaata gagtgccatt aacgcaccgg tttcaatccc tgccggggag
     1501 tcacaaacga taaattcaaa atccatcgct ttcagatcat caagaacttt ggcgacccct
     1561 tcacgggtga gggcatcttt atcgcgtgtt tgcgatgccg gaagaatata gagattttca
     1621 gtacgcttat ctttaattaa cgcctgattt agcgttgcat cgccctgaat gacgttgacg
     1681 aaatcgtaaa cgacccggcg ttcacaaccc ataatcaggt cgagattacg caggccgata
     1741 tcaaaatcta tcacgacagt tttctttccc ttctgggcca aaccagtggc gatggccgcg
     1801 ctggaggttg tcttaccaac cccccctttg cccgaagtaa caacaataat gcgtgccata
     1861 gaaattcctt gttaaaaagg gatcaattta acggttgaac ggtcaaagcg ttttcgacta
     1921 actgcagtcg cgccgctttg ccataaaatt ctgctgggat ttgatcactc agccagtatt
     1981 cacctgcgat ggacaccagt tccgccatca ggttcgtaca aaatatttgc gtttcccggt
     2041 caccacttgc ccctgcaagc gcacgaccgc gcatcatgcc atagacatga atgttcccat
     2101 cggcaatcaa ttcggcccca gcgctaacgt ggcttgtaac aatcagatca cattgtggag
     2161 cataaatacg ctgaccggaa cgcaccgggg tatctattaa acgcgttttt gtgaccggcg
     2221 ttgtattttg cgctggagcc tgtggtgcgg gagctggacg cggcgctttt tcctttcctt
     2281 ccgtcaggat aggcagcccc atcttttcaa tttcggcttt aagttgcgca tctttgcagc
     2341 cacttacgcc aataacccgc aaaccggttg ccgacaccgc cttatgcatc gctgaccagt
     2401 ttaccgggtc ttccagtgca ctgacgttga gtacaacggg ggcatgtttt aaaaatgcgg
     2461 gggcctgagc gattttgtct tccagcgcct gatggataac cttaggttct gcctcatgca
     2521 gatgaaccac agataaagtg aagctactgc ctttaagctc gattggcgtg tttgacatcc
     2581 tggccttact caattagcta ttaatcatcg ccagcgcgcg atgatgttcc gaagactata
     2641 aggcatgtta tagtctggat tatattgagg caagtcaccc tcccatttat tcagagtaaa
     2701 agtctattct gtgataaatg gcgctgattc atagcttaaa aaataccctt gtcaatcaac
     2761 ccattgccgt cgtacttttg attgttctta tttacgcttc ctttttccgc accctaatcg
     2821 tgacagcatc tccagacctg tttaaatatc cagtattgag gcaagtgtat tttttcacca
     2881 cgccaggctt tcttaacttt ccattttttg cagcatcatt aaagaacaga tacccgttat
     2941 cttaatatcc gccatagaat tgctctatca taatcataga aaccgcagaa gacgtctgat
     3001 acttctgtca ggtcatcgta actcgtccag gtcagcggtt ttgattacgg attgatgatg
     3061 aaatgagcat aagtgttgta cggttggatt atcaggatgt gctatgaata tgatgcgtat
     3121 tttttatatc ggattgtcag gtgtggggat gatgttctca tcaatggcgt ctggacatga
     3181 cgctggcgga cttcaatctc cggcgtgcgg cgttgtttgc gacccgtata tttgtgtgaa
     3241 ctcagatggc atttccccag agttaacaag gaaatatctc ggcgaaaaag ccgctgaaaa
     3301 cttacaatca ttacaaggct acgatcccag cgaatttaca ttcgctaacg gtgtattttg
     3361 cgatgttaaa gaaaaattat gtcgtgatga tcgttatttt ggtgtggatg gtaagcggag
     3421 tggaaaaatc aatcaaatca ccacaaagat gttatttatg tgtcgtgagt gatgccttgt
     3481 ttacattatg ttctat
//
LOCUS       DAZPEB010000272         3486 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZPEB010000272 DAZPEB010000000
VERSION     DAZPEB010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3486
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.277"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            1..1947
                     /locus_tag="ACHURJ_003996"
                     /pseudo
     CDS             1..1947
                     /locus_tag="ACHURJ_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
     gene            complement(1990..2436)
                     /gene="ivy"
                     /locus_tag="ACHURJ_003997"
     CDS             complement(1990..2436)
                     /gene="ivy"
                     /locus_tag="ACHURJ_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFR1282726.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            2617..3387
                     /gene="yafV"
                     /locus_tag="ACHURJ_003998"
     CDS             2617..3387
                     /gene="yafV"
                     /locus_tag="ACHURJ_003998"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFR1282727.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWQDADEFRLR"
BASE COUNT          778 a          891 c         1001 g          816 t
ORIGIN      
        1 atgattatca aaaaagagta cggctgactg gagttctcag cttatgccca gtctcgcgtg
       61 ctgcaaaaac tctccggcgt gagcgggatc ctggcgatta ccgtcggcgt gccgaactca
      121 ttaggcccgg gcgagctgct gcaacattac ggcactgacg agcagaaaga tcactatctg
      181 ccgcgtctgg cgcgtggtca ggagatccct tgctttgcac tgacaagccc ggaagcgggt
      241 tccgatgcgg gcgcgattcc ggacaccggg attgtctgca tgggcgaatg gcagggccag
      301 caggtgctgg ggatgcgtct gacttggaac aaacgctata ttacgctggc accgattgcg
      361 actgtgcttg ggctggcgtt taaactctcc gacccggaaa agctgctggg tggcgcagaa
      421 gatttaggta ttacctgtgc gctgatcccg accaccacgc cgggcgtgga aattggtcgt
      481 cgccacttcc cgctgaacgt accgttccag aacggaccga cgcgcggtaa agatgtcttc
      541 gtaccgatcg attacatcat cggtgggccg aaaatggccg ggcaaggctg gcggatgctg
      601 gtggagtgcc tctcggtagg ccgcggcatc accctgcctt ccaactcaac cggcggcgtg
      661 aaatcggtag cgctggcaac gggcgcgtat gctcacattc gccgtcagtt caaaatctct
      721 atcggaaaga tggaagggat tgaagagccg ctggcgcgta ttgccggtaa tgcctacgtg
      781 atggatgctg cggcatcgct gattacctac ggcattatgc tcggcgaaaa acctgccgtg
      841 ctgttggcta tcgttaagta tcactgtacc caccgcgggc agcagtcgat tattgatgcg
      901 atggatatca ctggtggcaa aggcattatg ctcgggcaaa gcaacttcct ggcgcgtgct
      961 taccagggcg caccgattgc catcaccgtt gaaggggcta acattctgac ccgcagcatg
     1021 atgatcttcg gacaaggagc gattcgttgc catccgtacg tgctggaaga gatggaagcg
     1081 gcgaagaaca atgacgtcaa cgcgttcgat aaactgttgt tcaaacatat cggtcacgtc
     1141 ggtagcaaca aagttcgcag cttctggctg ggcctgacgc gcggtttaac cagcagcacg
     1201 ccaaccggcg atgccactaa acgctactat cagcacctga accgcctgag cgccaacctc
     1261 gccctgcttt ctgatgtctc gatggcagtg ctgggcggca gcctgaaacg tcgcgagcgc
     1321 atctcggccc gtctggggga tattttaagc cagctctacc tcgcctctgc cgtgctgaag
     1381 cgttatgacg acgaaggccg taatgaagcc gacctgccgc tggtgcactg gggcgtacaa
     1441 gatgcgctgt atcaggctga acaggcgatg gatgatttac tgcaaaactt cccgaaccgc
     1501 gtggttgccg ggctgctgaa tgtggtgatc ttcccgaccg gacgtcatta tctggcacct
     1561 tctgacaagc tggatcataa agtggcgaag attttacaag tgccgaacgc cacccgttcc
     1621 cgcattggtc gcggtcagta cctgacgccg agcgagcata atccggttgg cttgctggaa
     1681 gaggcgctgg tggatgtgat tgccgccgac ccaattcatc agcggatctg taaagagctg
     1741 ggtaaaaacc tgccgtttac ccgtctggat gaactggcgc acaacgcgct ggcgaagggg
     1801 ctgattgata aagatgaagc cgctattctg gtgaaagctg aagaaagccg tctgcgcagt
     1861 attaacgttg atgactttga tccggaagag ctggcgacga agccggtaaa gttgccggag
     1921 aaagtgcgga aagttgaagc cgcgtaattg aataacggag ccgaaaggct ccgtttcttt
     1981 atccgctaat tatttaaaat taaagccatc cggatggttt tccaggctgc cggtcaacgc
     2041 cgcgaacagc accgttttac catcaatcga aagcgcatcg ttcacattca gccaggtgag
     2101 tttctcttgc gacgttttct catcaatagt cgagaacagc cccgtcatct gattagattt
     2161 ctcggaccac atcacagcga tacgttgcga gccacagtca tgcggtttgc acgcgctcat
     2221 cacctgatac gtctcatctc ccaacgttac ggtttgtgcg ggagtataag taccgccttt
     2281 catcacccag gcaggcagct tatgcccttg taccatctga ttaaatgcag ctttggtggt
     2341 ttcgcccttt gcaaggctgc taatggttaa atcatcctgc gccattgcac tggtggcgat
     2401 gaccagagcg gcgactgtcg ttattgcctt aaacatcatt cctcccgagc ttatcctgcc
     2461 catgttatta accttctaaa gcctggcgat aacacgataa tttgcaaatc agatatttcc
     2521 aatatcagca tttgttatca atttattctg cgctaaggca actgcctgat aaggtgttaa
     2581 agttataaaa cccttcgcat aaaggagttg tacaccgtgc ctggtttgaa gattacgctt
     2641 ttgcagcaac cactggtgtg gatggatggc cctgccaacc tgcgtcattt tgatcgtcaa
     2701 ctggaaggta ttaccgggcg cgatgtgatc gttctaccgg agatgtttac cagcggcttt
     2761 gccatggaag cggcagcttc gtcgctagca caagatgacg tagtgaactg gatgacagct
     2821 aaggcgcagc agtgcaatgc gctgatagca ggcagtgttg cattacaaac ggagtctggt
     2881 tcggttaacc gctttttgct ggttgagccg ggcggcacgg tacattttta tgataagcgt
     2941 catctgttcc gcatggcaga tgagcatcta cattataaag cgggcaatgc gcgagtgatt
     3001 gtggaatggc gcggctggcg tattttgccg ttggtgtgct acgacttacg ttttccggtg
     3061 tggtcgcgca atctcaacga ttatgacctc gccatctacg ttgccaactg gcctgctccg
     3121 cgctctctgc actggcaggc attgctgacg gcccgcgcga ttgagaatca ggcgtatgtg
     3181 gcgggatgca atcgcgtcgg cagcgatggc aacggctgcc attatcgcgg tgacagccgg
     3241 gtgattaatc cacaaggcga gattatcgct actgccgacg cgcatcaggc aacgcgcatt
     3301 gatgcggagc tgtcgatgat ggcattgcgg gaatatagag aaaagtttcc ggcatggcag
     3361 gatgcggatg agtttaggtt gcggtgagtg ggggttgtgt ttaaggctgg gggagagtcg
     3421 gggtaagact acgaaagccc gctctccgca aggactgacg ccagatagtt tctgtccata
     3481 gctgct
//
LOCUS       DAZPEB010000273         3411 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZPEB010000273 DAZPEB010000000
VERSION     DAZPEB010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3411
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.278"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..431)
                     /locus_tag="ACHURJ_003999"
     CDS             complement(<1..431)
                     /locus_tag="ACHURJ_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFR1282728.1"
                     /translation="MTIVARTEPGPAEDDITDTDDGDTRISAGAFWPDIVLRELRLAV
                     RLPGRVTTSRLLHTATGAVAHVTRELEAWQQEQQAAGHQTLADVPAPVINGESVNLWH
                     WRNAVYTATRALILERYRDADTTDKGDRRADALDIQTSDLWR"
     gene            complement(535..1345)
                     /gene="gpM"
                     /locus_tag="ACHURJ_004000"
                     /pseudo
     CDS             complement(535..1345)
                     /gene="gpM"
                     /locus_tag="ACHURJ_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632360.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
     gene            complement(1391..2263)
                     /locus_tag="ACHURJ_004001"
     CDS             complement(1391..2263)
                     /locus_tag="ACHURJ_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005136493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P2 family phage major capsid protein"
                     /protein_id="HFR1282729.1"
                     /translation="MIGVTDQKGEKILLDTTGPIARTNTSYDGTKRRNPNNVVDLKNR
                     KYQCEQVNYDTFISYPQLDAWSAHPDFQSRISAQIARQVALDRIMIGFNGTSHADESN
                     FSTNKLLQDVNVGWLEHIRTDASERVMNDVTLTSRNMDNTVAHAGKYANADALVQDAR
                     SSLLDEWHKEADDLVVIMGRNLFNSLRLPVLNSISGQNPNAELLAGQLILSSRTIGGL
                     GVFLAPFFPDATMLITSFNNLSIYWQKGSMRRLMKDEPEYNRIATYQSINDAYVVEDY
                     GKCAMVTGLKFADS"
     gene            complement(2260..2442)
                     /locus_tag="ACHURJ_004002"
     CDS             complement(2260..2442)
                     /locus_tag="ACHURJ_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P2 family phage major capsid protein"
                     /protein_id="HFR1282730.1"
                     /translation="MNLVMSDITRNKLGCYMAQQVSLNNIPVSALVSRFTVEPAVQQR
                     FENASKESTEFTKKIT"
     gene            complement(2466..3302)
                     /locus_tag="ACHURJ_004003"
     CDS             complement(2466..3302)
                     /locus_tag="ACHURJ_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFR1282731.1"
                     /translation="MANEKKTSRKKFRVAVSGSTVDGREISPVHLREAAENFNPDVYA
                     ARVNVEHYLSPCPSSEFSAMGDVTALSTEEITEGPLAGRTALYAEIEPTERMKQLVAD
                     GKKIYSSIELHPQFSVNGRAYLVGLAMTDTPASLGTERLKFTAQQRQAVMTFNSIQGE
                     APLISEAIESEIIEMAEQRQEEGTQWFNRVMGIIGRGRKADDASFSRIQEAVEGVATS
                     QADIIDRFNVLETRHQQDRQKITSLTTELAALKEKLRTQDGDPQNRFTATGAASDQLA
                     DF"
BASE COUNT          652 a          959 c          964 g          836 t
ORIGIN      
        1 cgccacaaat ccgatgtctg tatatccaga gcgtctgccc ggcggtcgcc cttgtccgta
       61 gtgtctgcat cacgataacg ctccagaatc agggcgcgcg tggcggtata aacagcattg
      121 cgccagtgcc agagattgac gctttctccg ttaattacgg gtgccggaac atcggccagc
      181 gtctgatggc cagctgcctg ctgttcctgc tgccatgctt ccagctcgcg ggtaacgtgt
      241 gccacagccc cggtggcagt atgcagcagg cgggaggtgg tcacgcgccc cggcagtcgt
      301 accgccagac gcagctcgcg cagcacaata tccggccaga atgcacccgc tgaaatgcgg
      361 gtatcgccat catcggtatc ggtgatgtcg tcctctgcgg gtccgggttc agttctggca
      421 actatagtca tggggttcac tcctgaaaaa aaatcgggcg gtgggtgcgc ggtgtaaacg
      481 gtcacggagt caaactggaa caccgcgcac gccgcccgct gacggggtca gtcgttaacc
      541 gcgcttcgcc ttctgcgtcg cggtggtttt tcgtgttgca ggcttccgcg ttgtcttttt
      601 acttttgctg ctttttgctg ctttcgtcct gcgcctgctg tgcgctggcg tcttctggtt
      661 cggctgcgga atcggctttt ttcagggcgc gggaaagggt tgcaatctcg cgtttcacac
      721 ctgcgttcgg gttcaggtgc attgcttcgc gcagcagctt cagtgacagg gccatgctgt
      781 ccgcatcact caggccacgg cgggcaaagg cgcacgcttt gcataatttg gcgcgcactt
      841 cgtccggcat gtcctggtcg gtgacaatct cccggagggt gtccagtggt tcgataaagg
      901 cggacaaatc cgcgtcggca tccgtcccgg cctgcgtcag taccggatta cagatttctt
      961 cggtcagtac cgtggcagca gtacggccaa agttatccgg catgatgagg ttgtgacgga
     1021 ctacatacgc gccgatacgc agcgcaagcg gaagatcgcc acagtcaatc gcccacacca
     1081 tcagcgtggc aatcacttca tcctgctgcc cgccgtcagc ctccagcgtt ccctcaatcc
     1141 agccggaaaa atccggcaac aactcttttt tgatggcggc tttcgcgctt ctggcctgta
     1201 cgcccttaag ccgggcctgt gccagacgca gacgatacag cacctcttca tgcgcggtac
     1261 gcgcggcgtg gtccacgcct tcattcgccc ggcctgcgcg ctgtgccatc acgttctgcc
     1321 agtgttgttg tgcaggagta atcatttttg ctctccgtta caggcgggca tgatgcccgc
     1381 cgtgagttga ttagctgtcg gcgaacttca ggccagtaac catcgcgcac ttgccatagt
     1441 cttcaacgac ataagcgtca ttgatggact ggtaggtggc gatgcggttg tattccggct
     1501 cgtctttcat caggcgacgc attgaacctt tctgccagta aatcgacagg ttgttgaacg
     1561 aggtgatcag catcgttgca tccgggaaga acggcgcaag gaacacgccc agcccgccaa
     1621 tggtgcgcga tgacaggatg agctgcccgg cgagtaattc cgcattggga ttctggccgc
     1681 tgatgctgtt cagcacgggc agacgcagcg agttaaacag gttgcgcccc ataatcacca
     1741 cgaggtcgtc agcttccttg tgccattcat ccagcaggga tgaacgcgcg tcctgtacca
     1801 gtgcatcagc gttcgcatac ttacccgcgt gcgccacggt gttgtccatg ttgcgggagg
     1861 tcagcgtcac gtcattcata acgcgttcgc tggcgtcggt tctgatgtgc tccagccagc
     1921 ccacgttaac gtcctgaagc agcttgttgg tgctgaagtt ggactcatcc gcgtgagacg
     1981 tgccgttgaa accgatcatg atgcggtcaa gcgccacctg tcgggcaatc tgtgcgctga
     2041 tgcgggactg aaaatcaggg tgtgccgacc aggcatcaag ctgcggatac gaaataaacg
     2101 tgtcgtagtt cacctgttca cactgatatt tgcggttttt cagatcaacc acgttattcg
     2161 ggttacggcg ttttgtgccg tcataactgg tattcgtgcg cgcaatcggt cctgtggtat
     2221 ccaggaggat tttttcgcct ttctggtcgg tcacgccgat cacgttattt ttttcgtaaa
     2281 ttcggtactt tcctttgagg cgttttcaaa acgctgctgc accgcgggtt ccacggtaaa
     2341 tcgcgacacc agtgcagata ccgggatatt attaagcgac acctgctgcg ccatatagca
     2401 acccagcttg ttgcgggtaa tatctgacat caccagattc ataaaaaatt tgctcctttg
     2461 tctaatcaga agtcggccag ctggtcggag gctgcgcccg ttgcggtgaa gcggttctgc
     2521 ggatcgccgt cctgcgtgcg cagtttttcc ttcagtgctg ccagctctgt ggtcagtgac
     2581 gtaattttct ggcggtcctg ctgatggcgg gtttccagca cattaaaacg gtcgataatg
     2641 tcggcctgtg acgttgcgac gccttccacc gcttcctgaa tacgggaaaa actggcgtca
     2701 tccgctttgc ggccacggcc aataatcccc attacgcggt taaaccactg ggtgccttct
     2761 tcctggcgtt gttctgccat ttcgatgatt tcagactcga tggcttcgga gataagcggc
     2821 gcttcaccct ggatactgtt aaacgtcatc accgcctgac gttgctgtgc cgtgaatttc
     2881 aggcgctcag tgcccaggct tgccggggtg tcggtcatcg ccagcccgac cagataggcg
     2941 cgcccgttaa cggagaactg cgggtgcagt tcgatactgg aatagatttt cttgccgtcc
     3001 gcaacaagct gcttcatgcg ctcggtcggt tcgatttctg catacagcgc agtacgtccg
     3061 gccagcggac cttccgtaat ctcttccgta ctcagtgcgg tgacatcgcc cattgcggaa
     3121 aattcgcttg acgggcatgg cgagagatag tgctcaacgt tcacgcgggc agcgtaaaca
     3181 tccgggttga agttctcggc ggcttcacgc agatgcaccg gactgatttc acggccatca
     3241 acagttgatc cggagacagc cacgcgaaac tttttgcggg atgtcttttt ttcattagcc
     3301 atagtttttg cccctctgac tggttcttca gtcatgatgg caaagcgtaa caggctgata
     3361 caaagggctt ttgttgtaag aaaacggcca gaacagaggg ttaaggagaa c
//
LOCUS       DAZPEB010000274         3409 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZPEB010000274 DAZPEB010000000
VERSION     DAZPEB010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.279"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(20..322)
                     /locus_tag="ACHURJ_004004"
     CDS             complement(20..322)
                     /locus_tag="ACHURJ_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282732.1"
                     /translation="MLEIVLSPVKAQQFTVTLGAQVCTIRLNQRTTGMYIDIIVNGEP
                     CLYGVLCLNNNRIVRYGYLPFQGDLFFTDTEGNSDPDWRGLGSRYRLYWLLPEELI"
     gene            complement(322..1032)
                     /locus_tag="ACHURJ_004005"
     CDS             complement(322..1032)
                     /locus_tag="ACHURJ_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000010340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HFR1282733.1"
                     /translation="MSFSFDNISLNNFSLSESNVLSAVRGGGVLGLINSVLAPSFGIY
                     YARNDPAGAHQKGGKPFSPDSFVVVEVGAEASVSTVPVEQGAYTTFNKIQRSPELHVT
                     FIVEGWTAFSGSVPNLTNFSTTSRTNVLETLERMRTTAGLYDIETPDKTWTSCDLVKY
                     DYRMRSNNGPTLLTVTAVFQTVMNTGEVSVGSTDKSSPTDNDKAKGAASVKTQPVTAS
                     VTQPSDADRRSATNRGIT"
     gene            complement(1029..3050)
                     /locus_tag="ACHURJ_004006"
     CDS             complement(1029..3050)
                     /locus_tag="ACHURJ_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFR1282734.1"
                     /translation="MAQVLDELVLSLGIDDRDFTAGEQAVHAALNRLTTVMEGVADTF
                     SQGQKKTSESLEKTGKDADKTARRMEDAGKRASRFFSGIRSEILALAGVSLTLGGLKN
                     LVTGFARDLNRLSVESDAFGMKARNLDGWLRAARANNVDEGEMSGAFSRLANAKAAFR
                     AGRSFDPVLQDLFQVAARAGISVDLNTDSTEAIMRKLAVIFPRLTKSEQTAYGNALGF
                     SYAGQQFLGSGHALKDVDEFTSRSQVTPGRTALARKLRKELVELDQTWSGIGMTISTA
                     LMPYALKLSHWLETLGDWMQQHPEEVNRFITTFLNDVESVASLANKAAGELGGWQNVI
                     ITLIGLKVASWVRGLTKALSGPGGLLFAITALYPVVDGLLTSIVGRENKDWLDSHGFF
                     WASDGTFFFNKKEMETYQTKLDVGEKPGNITRAQSPTVWQQGMLDTQASQATGREAAF
                     GESWLQGMRATQEKLGNAMQNRQRPTKTGEALLGWLQPKLSQLEEKYNLPPGLLRSVA
                     ITESRGNQFAVSPAGAMGLFQFMPKTASEFGLRGNDAFDPEKAADAAARKLGGLIRFF
                     HGDLAKALAAYNWGEGNVQRKGLAAAPEETRNYIPRVLANLPYPGAAMAIRSLHPAPV
                     SQSTVTETTHIGTLNVTTTSDNVKGITDDARRRITNSALVSVYSSGVTG"
     gene            complement(3034..3216)
                     /locus_tag="ACHURJ_004007"
     CDS             complement(3034..3216)
                     /locus_tag="ACHURJ_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFR1282735.1"
                     /translation="MVDYVNVPRTIATVISSGKASKAELDSVLGVQDLWDLLEIIHVD
                     AHNEQVIQENRNGAGT"
BASE COUNT          797 a          993 c          815 g          804 t
ORIGIN      
        1 attttacgtt gtctgtagct catatcagtt cctcaggtaa cagccagtag agccggtacc
       61 gggatccgag cccccgccag tcagggtcac tattcccctc cgtgtcggta aaaaacaggt
      121 ccccctgaaa cggcaggtat ccgtaccgga caatccggtt attattcaga cacagcacgc
      181 catacaggca cggttcacca ttaacaatga tatcgatata catccccgta gtacgctgat
      241 tcaggcgaat ggtgcagacc tgagcgccca gtgtcaccgt aaactgctgg gctttaacgg
      301 gagataaaac aatttccagc atcaggtgat ccccctgttc gtggcacttc gtctgtcagc
      361 gtctgacggt tgtgtcaccg acgccgtaac tggctgcgtt ttaaccgatg cggccccttt
      421 tgctttatcg ttgtccgtgg gagacgactt atccgtactt cccactgaca cctctcctgt
      481 attcattacg gtctgaaata ccgcagtaac cgtcagtaag gtcggaccat tattacttcg
      541 cattcggtag tcatatttca caaggtcgca ggatgtccat gtcttgtccg gcgtctcaat
      601 atcgtagagt cctgccgtgg tacgcatcct ttcaagcgtt tccagcacat tcgtccgcga
      661 ggtggtggaa aaatttgtca ggttcgggac ggatccggaa aaggccgtcc acccctctac
      721 aatgaaagtc acatgcagtt ccggcgatct ctggatttta ttaaaggtgg tataagcccc
      781 ctgctccacg gggacggtgg aaacagaagc ctccgcgccc acctcaacga caacaaaaga
      841 atccggggag aaaggtttgc cacccttctg atgggcacct gccggatcat tccgtgcgta
      901 ataaataccg aatgacggtg ccagtacact attaatgagt cccaggacac cgccgccacg
      961 aactgcactc agtacgttac tttcactgag cgaaaagtta ttcagggaaa tattatcgaa
     1021 agaaaaactc atcctgttac cccgctggaa taaaccgaca caagcgccga attcgtgata
     1081 cgccgacgtg cgtcatcggt aatacccttc acattgtccg acgttgtggt gacattcagt
     1141 gtcccgatat gcgtggtttc cgttacggtg gactgcgata caggcgcagg atggagcgac
     1201 cgtatggcca ttgccgcacc cggataaggc aggttcgcca gcacccgcgg aatatagttg
     1261 cgggtctcct ccggagcagc agccagtccc ttacgctgaa catttccctc tccccagtta
     1321 tatgccgcca gagccttagc cagatcgcca tgaaaaaaac gtatcaggcc accaagtttt
     1381 ctcgcggcgg catcagcggc tttttcggga tcaaaggcat cgttccccct cagaccaaat
     1441 tcactggccg tcttcggcat gaactgaaac agtcccatcg caccagcagg tgagacggca
     1501 aactgattac cacgggattc ggtgatcgca acgctgcgca gcagtccggg cggcaggtta
     1561 tatttttcct ccagttgaga cagtttcggt tgcagccagc ctaacagggc ttccccggtc
     1621 ttcgtcggtc gctggcggtt ttgcatggca ttaccgagtt tttcctgcgt cgcacgcata
     1681 ccctgtagcc aggattcacc gaaggctgct tccctcccgg ttgcctgaga agcctgagta
     1741 tccagcatgc cctgctgcca tactgtgggt gattgtgccc gggtgatgtt gccaggcttt
     1801 tctccgacat ccagttttgt ctgatacgtc tccatctctt tcttattgaa aaagaaagtc
     1861 ccgtctgaag cccagaaaaa accatgtgaa tccagccagt ccttattttc cctgccaaca
     1921 atggatgtca gtaatccgtc aacaaccggg taaagcgccg ttatcgcaaa aagaaggcct
     1981 ccggggccac tgagggcttt tgtcagcccc cgcacccatg acgccacttt cagcccgatc
     2041 agcgtaataa tgacattctg ccagcccccc agttctccgg cagccttatt cgccagggag
     2101 gccactgact caacgtcatt cagaaatgtg gtgataaacc tgttcacttc ctccggatgt
     2161 tgctgcatcc agtcaccgag tgtctccagc cagtggctga gcttcagcgc gtacggcatc
     2221 aacgcggtgc taatcgtcat cccgatgccg gaccaggtct gatcgagctc aacaagctct
     2281 tttcgtagct tacgggccag cgctgtacgc cccggcgtaa cctgcgaacg ggaagtaaat
     2341 tcgtccacat ctttcagcgc gtgaccggag ccaagaaact gctgtccggc ataactgaag
     2401 cccagcgcgt tgccgtaggc agtttgttcg gactttgtca gtcgcggaaa aataaccgcc
     2461 agcttgcgca tgatggcttc agtgctgtca gtatttaaat caacactaat gcccgccctg
     2521 gctgcaacct gaaacaaatc ctgtaacaca ggatcaaagg accttccggc tctgaaggcc
     2581 gcttttgcat ttgcaagccg ggaaaatgcc ccagacattt ccccttcatc aacattattt
     2641 gcccgcgcag cccgtaacca tccgtccaga tttctggcct tcatgccaaa agcatcggat
     2701 tccacggaca gacggttcag atcacgggca aaacctgtta ccaggttctt cagccccccc
     2761 agcgtcagac tgacacccgc aagagccaga atctcgctac ggatccctga aaaaaaacgg
     2821 gacgcccgtt tcccggcatc ttccattcga cgggccgttt tatcagcatc ttttccggtt
     2881 ttttccagtg attcactggt tttcttctgt ccctgactga atgtgtcagc gacaccttcc
     2941 atcaccgtcg tgagccggtt aagggcagca tgtactgcct gttccccggc agtaaagtcc
     3001 ctgtcatcaa tcccgaggga cagaacaagc tcatcaagta cctgcgccat ttctgttctc
     3061 ctgtatcacc tgttcgttat gggcatccac atgaattatt tccagcagat cccataagtc
     3121 ctgcacacca aggacagaat ccagttcagc ttttgaagcc ttaccggagg agataacggt
     3181 cgcaatggta cggggaacat taacgtaatc caccaccccg aacggtctgt cggggtcgag
     3241 ataacgcggg gggatatcta gctgacgacg gtagcgaaaa aatccacatg cagcttaaag
     3301 acctccgcac gcagattaag acgggtaatg atttcttcaa tatcctcttc aacaagcggt
     3361 ctccggatgc tgcgtttttt cggatcggga acaaactgca cacagtcca
//
LOCUS       DAZPEB010000275         3400 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZPEB010000275 DAZPEB010000000
VERSION     DAZPEB010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.280"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            110..946
                     /locus_tag="ACHURJ_004008"
     CDS             110..946
                     /locus_tag="ACHURJ_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFR1282736.1"
                     /translation="MANEKKTFRKKFRVAVSGSTVDGREISPVHLREAAENFNPDVYA
                     ARVNVEHYLSPCPSSEFSAMGDVTALSTEDITEGPLAGRTALYAEIEPTERMKQLVAD
                     GKKIYSSIELHPQFSVNGRAYLVGLAMTDTPASLGTERLKFTAQQRQAVMTFNSVQGE
                     APLISEAIESEIIEMAEQRQEEGTQWFNRVMGIIGRGRKADDASFSRIQEAVEGVATS
                     QADIIDRFNVLETRHQQDRQKITSLTTELTALKEKLRTQDGDPQNRFTATGAASDQLA
                     DF"
     gene            969..2021
                     /locus_tag="ACHURJ_004009"
     CDS             969..2021
                     /locus_tag="ACHURJ_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HFR1282737.1"
                     /translation="MNLVMSDITRNKLGCYMAQQASLNNIPVSALVSRFTVEPSVQQR
                     FENAVKESTEFTKKINVFGVTDQKGEKILLDTTGPIARTNTSYDGTKRRNPNNVVDLK
                     NRKYQCEQVNYDTFISYPQLDAWAAHPDFQSRVSTQIARQVALDRIMIGFNGTSHADE
                     SNFSTNKLLQDVNVGWLEHIRTDASERVMNDVTLTSRNMDNTVAHAGKYANADALVQD
                     ARSSLLDEWHKEADDLVVIMGRNLFNSLRLPVLNSISGQNPNAELLAGQLILSSRAIG
                     GLDVFLAPFFPDATMLITSFNNLSIYWQKGTMRRLMKDEPEYNRIATYQSINDAYVVE
                     DYGKCAMVTGLKFADS"
     gene            2067..2867
                     /gene="gpM"
                     /locus_tag="ACHURJ_004010"
     CDS             2067..2867
                     /gene="gpM"
                     /locus_tag="ACHURJ_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFR1282738.1"
                     /translation="MITPAQQHWQNVMAQRAGRANEGVDHAARTAHEEVLYRLRLAQA
                     RLKGVQARSAKAAIKKELLPDFSGWIEGTLEADGGQQDEVIATLMVWAIDCGDLPLAL
                     RIGAYVVRHNLIMPDNFGRTAATVLTEEICNPVLTQAGTDADADLSAFIEPLDTLREI
                     VTDQDMPDEVRAKLCKACAFARRGLTDADSMASSLKLLREAMHLNPNAGVKREIATLS
                     RALKKADSAAAPEDASTPQAQDESSKSKKTTRKPATRKTTATQKAKRG"
     gene            2970..>3400
                     /locus_tag="ACHURJ_004011"
     CDS             2970..>3400
                     /locus_tag="ACHURJ_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFR1282739.1"
                     /translation="MSMVARTEPRPAEDDITDTDDGDTRISAGAFWPDIVLRELRLAV
                     RLPGRVTTSRLLHTATGAVAHVTRELEAWQQEQQAAGHQTLADVPAPVINGESVNLWH
                     WRNAVYTATRALILERYRDADTTDKGDRRADALDIQTSDLWR"
BASE COUNT          836 a          966 c          956 g          642 t
ORIGIN      
        1 gttctcctta accccctgtt ctggccgttt tcttacaaca aaagcccttt gtatcagcct
       61 gttacgcttt gccatcatga ctgaagaacc agtcagaggg gcaaaaacta tggctaatga
      121 aaaaaagaca ttccgcaaaa agtttcgcgt ggctgtctcc ggatcaactg ttgatggccg
      181 tgaaatcagt ccggtgcatc tgcgtgaagc cgccgagaac ttcaacccgg atgtttacgc
      241 tgcccgcgtg aacgttgagc actatctctc gccatgcccg tcaagcgaat tttccgcaat
      301 gggcgatgtc accgcactga gtacggaaga cattacggaa ggtccgctgg ccggacgtac
      361 tgcgctgtat gcagaaatcg aaccgaccga gcgcatgaag cagcttgtcg cggacggcaa
      421 gaaaatctat tccagtatcg aactgcaccc gcagttctcc gttaacgggc gcgcctatct
      481 ggtcgggctg gcgatgaccg acaccccggc aagcctgggc actgagcgcc tgaaattcac
      541 ggcacagcaa cgtcaggcgg tgatgacgtt caacagtgtc cagggtgaag caccgctcat
      601 ttccgaagcc atcgagtctg aaatcatcga aatggcagaa caacgccagg aagaaggcac
      661 ccagtggttt aaccgcgtaa tggggattat tggtcgtggc cgcaaagcgg atgacgccag
      721 tttctcccgt attcaggaag cggtggaagg cgtcgcaacg tcacaggccg acattatcga
      781 ccgttttaat gtgctggaaa cccgccatca gcaggaccgc cagaaaatca cgtcactgac
      841 cacagagctg acagcactga aggaaaaact gcgcacgcag gacggcgatc cgcagaaccg
      901 cttcaccgca acgggcgcag cctccgacca gctggctgac ttctgataag acaaaggagc
      961 aaatttttat gaatctggtg atgtcagata ttacccgcaa caagctgggt tgctatatgg
     1021 cgcagcaggc gtcgcttaac aatatcccgg tatctgcact ggtatcgcga tttaccgtgg
     1081 aaccctcggt gcagcagcgt tttgaaaacg cagtaaagga gagcactgaa tttacaaaaa
     1141 aaattaacgt gttcggtgtg actgaccaga aaggcgaaaa aatcctcctg gacaccaccg
     1201 ggccgattgc gcgcacgaat accagttatg acggcacaaa acgccgtaac ccgaataacg
     1261 tggttgatct gaaaaaccgc aaataccagt gcgaacaggt gaactacgac acgtttattt
     1321 cgtatccgca gcttgatgcc tgggcggcac accctgattt tcagtcccgc gtcagcacac
     1381 agattgcccg gcaggtggcg cttgaccgca tcatgatcgg tttcaacggc acgtctcacg
     1441 cggatgagtc caacttcagc actaacaagc tgcttcagga cgttaacgtg ggatggctgg
     1501 agcacatcag aaccgacgcc agcgagcgcg ttatgaatga tgtaacgctg acctcccgca
     1561 acatggacaa caccgtggcg cacgcgggta aatatgcgaa cgctgatgca ctggtacagg
     1621 acgcgcgctc atccctgctg gatgaatggc acaaggaagc tgacgacctc gtggtgatta
     1681 tggggcgcaa cctgtttaac tcgctgcgtc tgcccgtgct gaacagcatc agcggccaga
     1741 atcccaatgc ggaattactt gccggacagc tcatcctgtc atcgcgcgcc attggcgggc
     1801 tggatgtatt ccttgcgccg ttcttcccgg atgcaacgat gctgatcacc tcgttcaaca
     1861 acctgtcaat ttactggcag aaaggaacaa tgcgtcgcct gatgaaagac gagccggaat
     1921 acaaccgcat cgccacctac cagtccatca atgacgctta tgtcgttgaa gactatggca
     1981 agtgcgcgat ggtcactggc ctgaagttcg ccgacagcta atcacctcac ggcgggcatc
     2041 atgcccgcct gtaacggaga gaaaaaatga ttactcctgc acagcaacac tggcagaacg
     2101 tgatggcaca gcgcgcaggc cgggcgaatg aaggcgtgga ccacgccgcg cgtaccgcgc
     2161 atgaagaggt gctgtatcgt ctgcgtctgg cacaggcccg gcttaagggc gtacaggcca
     2221 gaagcgcgaa agccgccatc aaaaaagagt tattgccgga cttttccggc tggattgagg
     2281 gaacgctgga ggctgacggc gggcagcagg atgaagtgat tgccacgctg atggtgtggg
     2341 cgattgactg cggcgatctt ccgctggcgc tgcgtattgg tgcatatgtg gtccgtcaca
     2401 acctcatcat gccggataac tttggacgta ctgctgccac ggtactgacc gaagaaatct
     2461 gtaatccggt actgacgcag gccgggacgg atgccgacgc ggatttgtcc gcctttatcg
     2521 aaccactgga caccctccgg gagattgtca ccgaccagga catgccggac gaagtgcgcg
     2581 ccaaattatg caaagcgtgc gcctttgccc gccgtggcct gaccgatgcg gacagcatgg
     2641 cctcatcact gaagctgctg cgcgaagcga tgcacctgaa cccgaacgca ggtgtgaaac
     2701 gcgagattgc aaccctttcc cgcgccctga aaaaagccga ttccgcagcc gcaccagaag
     2761 atgccagcac accgcaggcg caggacgaaa gcagcaaaag taaaaagaca acgcggaagc
     2821 ctgcaacacg aaaaaccacc gcgacgcaga aggcgaagcg cggttaacga ctgaccccgt
     2881 cagtgggcgg cgtgcgcggt gttccggtct gactccgtga ccgtttacat cgcgcaccca
     2941 ccgcccgatt tttttcagga gtgaacccca tgagtatggt tgccagaact gaacccagac
     3001 ccgcagagga cgacatcacc gataccgatg atggtgatac ccgtatttca gcaggtgcat
     3061 tctggccgga tattgtgctg cgtgagctgc gtctggcggt acgactgccg ggccgtgtga
     3121 ccacctcccg cctgctgcat accgccaccg gggccgtggc acacgttacc cgcgagctgg
     3181 aagcgtggca gcaggaacag caggcggctg gccatcagac gctggccgat gttccggcac
     3241 ccgtaattaa cggagaaagc gtcaatctct ggcactggcg caatgcggtt tacaccgcca
     3301 cacgcgccct gattctggag cgttaccgcg atgcggacac aacggacaag ggcgaccgcc
     3361 gggcggacgc actggatata cagacatcgg atttgtggcg
//
LOCUS       DAZPEB010000276         3383 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZPEB010000276 DAZPEB010000000
VERSION     DAZPEB010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.281"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(55..2707)
                     /locus_tag="ACHURJ_004012"
                     /pseudo
     CDS             complement(55..2707)
                     /locus_tag="ACHURJ_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415720.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT          843 a          793 c          782 g          965 t
ORIGIN      
        1 gctgcttaaa aaaagaacaa atggtagtgt ccgctattgc cagtacacct cattcacata
       61 gacattcagc tggctgttgc ctgcggtgat gtcatacccc agtatcatgc tggcacgccc
      121 cagcagcgat tcgtagtgat tcagatgtat attgaggcca ttactcgcct tcatagccat
      181 ctcattctgg tggctgtatg taagctgggt ttgcggctct atatagaacc cataaccagt
      241 aggggacagg ttgaacctct gcccggcttc tagggagatg ctcattccat tcgcagtgcc
      301 gttggcgtta acgccgttgt tatgactgtc cagtacgtgg aaactatttt tctggcgcga
      361 tgcttttata acgagatcgc tgtaaaaacc gttttgtgcc atgtaactgg cgtacattcc
      421 catgtagtct gaacgtgcgg taccgtcgcc tccgctatag tccggcgatg catgtgttga
      481 gccaatatac agaccgacat acaacggcat ttcatcagag agacgtttat ccccaccaaa
      541 ctggataccg ctgtaaccca tgtcaaagcc gctcagtttg ccactggcaa aggagtccag
      601 gcttccccca taactgcgca accagatatt accgtcttta ctctgatttc gcaggtcacc
      661 catccgttgc atcagcgtac ggttttcaac atagttcaat aagtaaccga cattgagata
      721 attgccgcca gcatctgcgg tcgtggtggg tttaggcgtg ggagcgggtt caggcgtagg
      781 atcggggttg actatgggag gctgagcggg agctggtgtg ggttcaggat taggagtcgg
      841 ttccggaact gtcccggaag cgtaaagctc ccagttagtg ccatttttac gcacatcgta
      901 cagatatccc ccccaactca acctgagaag acgcgctgaa cgaggccgcg ccgtcagtgg
      961 tttttaccac tgtcagaact tcatttcccg ttgtggcctc gctgccctgg ttgcggatag
     1021 ccaatacgtg attaccagca ctactcccgc tgatattcaa taaatcccct ttattattaa
     1081 cgccattacc ctcgccaaca acatcagcac gcataataaa ggtactgtta ccgctcaggt
     1141 tctctacgtt taatgtggca aatgtgccgg cagttgaccc gtggctggca aaatcgacag
     1201 ttgaatggct cagcgccagc gtgtcgagat tagaattact tgttacgttc cagacgctgt
     1261 tattcattgc aacgtccagt tttccgccat tgacattatc gctgagggag gaacctgtcc
     1321 aaaccgaccc agggtgcata tccagattga ttagcccacc tttggaaaaa acgctaccgt
     1381 tgataagcat acgacccgag gcgttgatgc ggctggcggc ataaccatcg tcatgttgcg
     1441 ttgcaatggc catctggtcg ggtgtactca tatcaatgac cagatcgctc gtgaggtcga
     1501 tctggctgtt ggtcatggca taaattcccc tggctcctgc cgcgccggtg atagccaaac
     1561 tgtctccggt tattctaccg ccgctgagcg cccacaaacc cagcgccaga ctgccattac
     1621 gatcaactgt aatatcggta ttttgcatgt tgacaacagc cgttgccgtc tgggccgagg
     1681 caccataaga accgccggaa aagatgctgt ttcgttgcgc tgccgtgcca gaaaaattga
     1741 ttgtcgcgtc tgaaccactg gtgacgaggc caccgccctg cgcggaagaa atatggctat
     1801 ctgcaccgat agtggttgta ccaccacgaa cttcgacgcc attggccgcg gctccagtta
     1861 catcaacagt gagtgcattc gcgctaacct ggccaaagct ccagaggccg tgtgcattat
     1921 cgccattggt tttaatggaa ctgtttgttc cgagatcgac aacagagttt ttctgtacgt
     1981 ttatccccat ggcgctgtag ccctgaacat cgattgtcag gtctgtagcc gtaaaacgcg
     2041 cagcaccatt ggcgttattg ccgttgagac caccgatata aacacctgta cttccatcgg
     2101 tcgtgatttt acttccgctt ccaagatcga cactggtgcc atagtcattg atggttaggc
     2161 ctataccgtt cgagttttca atggtgaatt gagtggctgt tagtgttgag ctatgcccaa
     2221 taatgatgcc gtcatcgtta ctcttaatgg tgctgcctgt gcctaagtca gcatgggtat
     2281 agtcaccaat taagttaatg ccgatggcag aggtttgacc aacaacatct actgtgagtc
     2341 ggttagctgt cagggagctg tttttgccct gaataatgat tccttttgca tatccagagg
     2401 cgtcgttgac attgacggtg acatcattac ccaggttgat attgccaggc gtgtttcctg
     2461 ctggggtcat tacaccatag aggtttcctg atggatcggc ggtatctcct gtaattctgt
     2521 caccatcatt aatagtaata ttggtgccat attgacttcc tgtatcagca gctatcagca
     2581 ggggggaaaa gataattgca ttcaaaccaa tagcgccaaa ttttatcagt tgaattgagg
     2641 gcgaacggat ttttaattca gcgagacgat ctgctttggt attcccattg tgttgtttga
     2701 tgcccatacc actcctatat agtacccaga ctatgggtct atggattttg tcattttgtt
     2761 taaatcaatg aataatatcc tcttatcatc atgatatttg tatggtgcaa tcatgatgag
     2821 cttctcagag aataattctc tattaccggg caaaatcagg aacacaggaa ttaatatata
     2881 tttaacatcc cccttacaaa ggtgagttta tatcgagtta ttccctaagt tgtcgttact
     2941 tattccaatt cgttactata aatatggcaa aaatattaca acaaacagcg gttaaaaaat
     3001 ttggtgaaga ttttttgtaa taatcctaat tagttatggg aatttcgctt gatgtatctg
     3061 cataacaaaa acaatatgcg ccatttttgc attactaata agaaaaagca aaaaacatcg
     3121 atattcattt ttggtcaatg ggttttctgg cctatcgttg ttattgtgct actggttatc
     3181 attttcatct tgccagcata ttggagcgtg atcaattttg atcagctgtg aaggtggctg
     3241 atgtgtagtg atacaaaaac ttacgttata tcactgctaa tgagatatcc gtcatctgaa
     3301 cacttatgtc tttaattatt ctcgtggttc actataggca ataagcacaa aagtgtagga
     3361 tgttacaaga atgattagga ctc
//
LOCUS       DAZPEB010000277         3321 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZPEB010000277 DAZPEB010000000
VERSION     DAZPEB010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.283"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..212)
                     /locus_tag="ACHURJ_004013"
     CDS             complement(<1..212)
                     /locus_tag="ACHURJ_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282740.1"
                     /translation="MIRFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVCISLQELQLELMVEYALFF"
     gene            complement(215..982)
                     /locus_tag="ACHURJ_004014"
     CDS             complement(215..982)
                     /locus_tag="ACHURJ_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282741.1"
                     /translation="MQPSPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERAILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNVITTQINAWARNNHPLLKERARQAIEN
                     PLKQPEPMTEYQVWEDDWIIDECLCAMYDASSESLGK"
     gene            complement(985..1695)
                     /locus_tag="ACHURJ_004015"
     CDS             complement(985..1695)
                     /locus_tag="ACHURJ_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282742.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYDVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(1711..3216)
                     /locus_tag="ACHURJ_004016"
                     /pseudo
     CDS             complement(1711..3216)
                     /locus_tag="ACHURJ_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472437.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
BASE COUNT          934 a          797 c          729 g          861 t
ORIGIN      
        1 ctgaaaaaaa gtgcatattc aaccattaac tcaagttgta attcctgaag agaaatacac
       61 acaaccagga gcggaaagga gaatattatt tctgccagaa aagttaacgt gaatataagt
      121 acggctaaga caataaccgt cggccatacc aataatggca cgataacaaa accgagaatt
      181 acagggggaa tataaagccg gaatctaatc atttttattt tcctagtgat tcggatgatg
      241 cgtcatacat cgcgcataaa cactcatcaa ttatccagtc atcttcccag acctgatatt
      301 ctgtcattgg ttcgggctgt tttagcgggt tctcaatcgc ttgccgcgct ctttctttca
      361 gtaaaggatg attatttcta gcccaggcat tgatttgggt ggtaatgacg ttatcgatat
      421 cgtcctggtt atctctgata taatcctcac aatatctgtt caataaacag gcaagagaaa
      481 cggtcgacca aaagccggga actgaattaa tggcatccag caatggcgca agagcgatat
      541 cggcacggaa aaacatttcc agcatatact gtaattcgac agcttcatcg ccggataccc
      601 aatcagtcat atactggcag gcaaatcgct gtaaaattgc ccgttcatcc gcacgccagt
      661 tttctttgtt ggcaaggttg atacggcgca gagaaaaaac tacgtgaata ttgtcaaaat
      721 gcccctgtgc aacaaattcc agtaaacgcg gcagaaaata gcggacctca tcgctatttt
      781 gcggatcagg ccctgggcta ctattccagg cattgattaa ggaatacggg atcgctcgta
      841 acgatgtact ccgtaatgcc ttttgttcct gctgactcag gcaatattca caacaaacca
      901 caaatttctg cggcacccga taacgacgaa aggtgtgata aatctgctca attaatgcct
      961 gcatggcggg gctgggttgc atagttaccc cctcgcccgc tgatcgcgtg taaacggttg
     1021 atagacaaaa cgagcattcg ccgcgttcag gcgaataagg gcgctacgcg aaagggaaaa
     1081 gctggggcga atgcaactat tgcagtctcg acagctttcc tctgcgcaat tttccactga
     1141 gtaatgacgc gtgtagagtt tcagttcggc ctgcttatcg cgaaatccgc gtaaactgtt
     1201 attgttatga aatttccagg catctttaaa gacctgaaag atatagacat cctcaatcca
     1261 ggagtcacat tttttaagcg ggcgctttgc tattatcacc cgctcatccg gatttagcat
     1321 cgccgtaaat tgtacggtga cgtcatttgt cgtgttcagg tcacattgaa catctggtac
     1381 atcactgata taggggtcaa atccaaaatc agcaccacag gcacaaagac ggctaatggt
     1441 ggcggtatcg agggaaaatc ccggccatac tttgctggag gttaagccaa tatttaatgt
     1501 gacatcatat cgaagtaatc cctcaagacc gtcagcaccg acgaactttt ccccttcatc
     1561 cagggcctgg ttaataagtt gctgaacaat gccttcatcc cagtatgccg atttgcagtt
     1621 ggcaatcgta atatccacgc ctgtgaaggg atcagtaccg gcaaggcttg caaataatgt
     1681 attaactaat tccattttta ttatccagct ttaaggtagc gtcttataca tcgggacata
     1741 acctacatct tccaccaaac tctgtccctg cggcgttaaa aaccactcca acaatttttg
     1801 tgtctctgac gtcgtatttt cccgcgttac cataaaggca tcgatgatat agggatattt
     1861 gccgttgcga atgttttcgg cagtcggtgc aataccgtta atcgctagca gttttatatt
     1921 tttatcggcg ttcatttgtg ttgcgtaata acggaaggtg taacctattg cgttgtttgt
     1981 attacggtat tcggcaacga ctttaatcat tccctccatc atacttgcca cttctgtttc
     2041 ctgcggcgag atcatgcgga catttttcat gacctgggat tgcatcaccg tctggctacc
     2101 agagtcttcc gggcgttgct aggtctggat ctcctgatcg ttaccgccaa cagtacgcca
     2161 gttggtaatt gcaccactga agatgtcacg cacctgttgt tcagtcaggg aattaaccgg
     2221 attatccgca ttgacgatga aaacaaacgc ttcacgggca aaaggcgtgt acagcaaagt
     2281 aatgcccgat tcctcagcgc gttttttctg cccaccggaa ggttgcgcca caaaaataat
     2341 atcagcatca cctttaacaa tcctgttata cgcatcgggg gtacgagagc tctccagata
     2401 ttccctggtg tgaaaatctt ccggtataac gctcaaagca taaaatgcag aggcataaat
     2461 tgggtacgcc gccgtagcac catcgagtcg cggccagttt tgcgtaaact gaatttgcgg
     2521 tttaccacgc agtggtgtca gttgattatt gagtttatcg ggtcgccagg cccaggtatc
     2581 aatattttcg cgcacaccga cgccgggatt gagctgttta tataaataac cctgataacc
     2641 agcgacaaaa ccaagcatga gaaggataaa caggagggaa ttacggcaac gtagcccgga
     2701 tgtaccggtt aaaggatgtc tggttggccg gccgtacccc agcgaaaaac tgatatgcgc
     2761 gccaacgggt ataatgacta tccaccagta ctcacctccg actactgaga ctaacaaaac
     2821 gcccagccag gggagcataa gatcagccaa ccaacccgat cccatctggt gaaattgata
     2881 atctgccaga tgcagcgcga ttatccagcc aaccaggatt aataaaccgg gtactaaaaa
     2941 aagacattgt cgccagacgg ctatttctct tttttctggt gcactatcgc ctgcccatac
     3001 tcgccccgag aaaaatagca ttaatgcgcc cagcgccagc ataaaaatgt tgagcagaag
     3061 gtaacttgtt ggcgtgataa ggatattaca aacttcaata ataccggcaa gtccgacacc
     3121 cagcatagca acaaccgccg ccaaaaattg cgtcagtatg gggatgccga aaaaagtcat
     3181 taccagcgag gacaaaatcc attttctgtt ttgcattatt ctttccattc tttttgaatg
     3241 gtgaaattat actccccgag tccccttgcc ccttctggac acttttccga aatgatggcg
     3301 gaaaaaaacg ggaccctttg g
//
LOCUS       DAZPEB010000278         3287 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZPEB010000278 DAZPEB010000000
VERSION     DAZPEB010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3287
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.284"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            137..583
                     /gene="hpaR"
                     /locus_tag="ACHURJ_004017"
     CDS             137..583
                     /gene="hpaR"
                     /locus_tag="ACHURJ_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HFR1282743.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEAIPGDNE"
     gene            702..2357
                     /gene="tsr"
                     /locus_tag="ACHURJ_004018"
     CDS             702..2357
                     /gene="tsr"
                     /locus_tag="ACHURJ_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFR1282744.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQRETSAVVKTVTPAAPRKMAVADSEENWETF"
     gene            complement(2406..>3287)
                     /locus_tag="ACHURJ_004019"
     CDS             complement(2406..>3287)
                     /locus_tag="ACHURJ_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1282745.1"
                     /translation="DWFNIKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFI
                     TIGVLGIFLAIGWYMLYRNREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRT
                     MWGMMLGFSGINYTAWLYLAWLPGYLQTAYNLDLKSTGLMTAFGAAGMLVNGYVTDWL
                     VKGGMAPIKSRKICIIAGMFCSAAFTLIVPQATTSMTAVLLIGMALFCIHFAGTSCRG
                     LIHVAVASRMTASVGSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGA
                     LAYIFLVRQPINDPRKD"
BASE COUNT          852 a          805 c          915 g          715 t
ORIGIN      
        1 aatattaaat atcagtaagt taataaaata tcatcaattt tgataaaatc aggaattgca
       61 ggggcagcgc agtcccggta acgtgacgat taactgtttc tatattaatg attttgaatt
      121 tttcagggga aagataatgc acgactcact aaccattgcg ttgctccagg cgcgagaagc
      181 ggcgatgagt tattttcgcc caatcgttaa acggcataat ttgactgagc aacagtggcg
      241 catcgtgcgt attctggcgg aaagcccatc aatggatttt cacgatctgg cgtatcgcgc
      301 ctgcattttg cgcccaagcc tgaccgggat tctcacgcgg atggaacgcg acggtttagt
      361 gttgcgatta aagccgatca atgatcagcg gaagctctat atctcgctaa ccaaagaggg
      421 gcaggcgctg tataaccgcg ctcagacaca gattgaagaa gcctatcggc agattgaagc
      481 tcagtttact gccgagaaaa tgcaacagtt aacccatctg ttagaggaat ttattgctct
      541 gggcaattcc cgacaagaag ccatcccggg cgataatgaa taacttttta gtatctgata
      601 accagcgcct ggtttttcca tggatggcgg gttacacctt ttcataaagt ttttgctttc
      661 caagccgaaa atcttgcatc ggtccacagg aaagagaaac catgttaaaa cgtatcaaga
      721 tcgtgaccag cttactgctg gttttggccg tttttggcct tttacaactg acatcaggcg
      781 gtctgttctt taatgcctta aagaatgaca aagaaaattt cactgtttta caaaccattc
      841 gccagcagca atccacgctg aatggcagct gggtcgcgtt gttgcagacg cgtaacaccc
      901 tcaaccgcgc gggtatccgc tacatgatgg atcagaataa tattggtagc ggttcaaccg
      961 ttgctgagct gatgcagagt gccagtattt cgctgaaaca ggcggaaaaa aactgggcgg
     1021 attacgaagc gttgccgcgt gacccgcgtc agagcaccgc cgcagcggca gagatcaaac
     1081 gtaattacga tatttatcac aatgcgctgg cggagctgat ccaactgtta ggtgcaggca
     1141 aaatcaacga gttctttgat cagccgaccc agggatatca ggacggtttc gagaagcagt
     1201 atgtggctta catggagcaa aacgatcggc tctatgatat cgccgtcagc gataacaatg
     1261 cctcctacag ccaggcgatg tggattctgg tgggcgtgat gatcgtcgta ctggcggtca
     1321 tcttcgccgt ctggttcggt attaaagcct cgctggtagc gccaatgaat cgcctgattg
     1381 acagcattcg tcatattgca ggcggcgatc tggtgaaacc gattgaggtg gatggctcta
     1441 atgagatggg gcaactggca gagagtttgc gccatatgca gggagagctg atgcgtaccg
     1501 tcggtgatgt gcgcaacggg gccaatgcca tctatagcgg tgccagcgaa atcgccaccg
     1561 gcaataacga tctctcttcg cgcaccgagc aacaggccgc ttcgctggaa gagacggcag
     1621 ccagcatgga gcaactgacc gcaacggtga agcagaacgc cgaaaatgcg cgccaggcca
     1681 gccatctggc gttaagtgct tctgaaacgg cgcaacgcgg cggcaaagtg gtagataacg
     1741 tggtacagac tatgcgcgat atctccacca gttcgcagaa aatcgccgat attatcagcg
     1801 taattgacgg cattgccttc cagaccaata ttctggcttt gaacgcggcg gttgaggctg
     1861 cgcgtgcggg tgagcaaggg cgcggttttg cggtggtcgc gggagaagtg cgtaatctgg
     1921 cccagcgcag cgcccaggcg gctcgtgaaa ttaaaagcct gattgaagac tcggtgggga
     1981 aagtggatgt tggctctacg ctggtcgaaa gcgccgggga aacaatggcg gagattgtca
     2041 gcgccgtgac ccgcgtgacg gacattatgg gcgaaattgc ttctgcttct gatgagcaga
     2101 gccgtggtat cgatcaggtt ggcttagcgg ttgctgagat ggaccgggta actcaacaga
     2161 acgccgcgct ggtggaagag tctgccgctg ccgccgccgc gctggaagag caggccagtc
     2221 gcctgaccga agcagtggca gtgttccgga ttcagcaaca gcagcgtgaa acatcggctg
     2281 tggtaaaaac cgtgacgcca gctgcgccgc gtaaaatggc cgtggcagat agcgaggaga
     2341 actgggaaac attttaatcg ccatgaaaat gcccgataag caaaatgtta tcgggcataa
     2401 ggagattaat ctttacgtgg gtcgttgatc ggctgacgaa ccaggaagat gtacgccagt
     2461 gcccccgctg cggtgacgca accgcagatg attagtgcca gacggaatga gtgggtggta
     2521 tcaacaataa aaccagtaat gatcggcgca aaagaggcgc agatgaagct ggcaaagttc
     2581 tggatactgc ccaccgacgc agtcatgcga gaagcaactg cgacgtggat caagccccgg
     2641 caggatgttc cggcaaagtg aatacagaac agtgccatgc caatcagcag aaccgccgtc
     2701 atggatgttg tcgcttgtgg tactatcagc gtaaaggcgg cagaacagaa catcccggca
     2761 ataatgcaga tcttacggct tttaatcgga gccattcccc ctttgaccag ccagtcggta
     2821 acgtaaccgt tgaccagcat cccggcagcc ccaaacgccg tcatcaaccc tgtgcttttt
     2881 aaatccaggt tataggctgt ttgcaggtaa ccaggaagcc aggccagata cagccacgca
     2941 gtgtagttga tgccactgaa tccgagcatc attccccaca ttgtacggtt acggaacagg
     3001 ctgcgccatt cggcaaaact gagcggatct cggcgggcat tgacgctacc tgcattgaga
     3061 taagcttgct caacggcagt cagttctacg tgctcgcggt tgcgatagag catataccag
     3121 ccgatggcga gaaaaatccc cagtacacca atggtaataa acatcccgcg ccagcccatc
     3181 accagcatca tcgccgccag aatcggtggg cttacggcaa cgccaatggt agaagctgcg
     3241 ttgaagaagc ccatcgggcg tccgcgctct ttgatgttga accagtc
//
LOCUS       DAZPEB010000279         3235 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZPEB010000279 DAZPEB010000000
VERSION     DAZPEB010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.285"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(1..66)
                     /locus_tag="ACHURJ_004020"
                     /pseudo
     CDS             complement(1..66)
                     /locus_tag="ACHURJ_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858254.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            135..1115
                     /locus_tag="ACHURJ_004021"
     CDS             135..1115
                     /locus_tag="ACHURJ_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS5 family transposase"
                     /protein_id="HFR1282746.1"
                     /translation="MSHQLTFADSEFSSKRRQTRKEIFLSRMEQILPWQNMVEVIEPF
                     YPKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLFARLSLDSALPDR
                     TTIMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIEAPSSTKNKEQQR
                     DPEMHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVS
                     ADAGYQGAPQREELAEVDVDWLIAERPGKVRTLKQHPRKSKTAINIEYMKASIRAKVE
                     HPFRIIKRQFGFVKARYKGLLKNDNQLAMLFTLANLFRADQMIRQWERSH"
     gene            complement(1445..1588)
                     /locus_tag="ACHURJ_004022"
     CDS             complement(1445..1588)
                     /locus_tag="ACHURJ_004022"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282747.1"
                     /translation="MSLDPLACGHDNVKQSKIVALTVSLGDKKAQYSRWQPDNLTVDA
                     ETK"
     gene            complement(1931..2160)
                     /locus_tag="ACHURJ_004023"
                     /pseudo
     CDS             complement(1931..2160)
                     /locus_tag="ACHURJ_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            2747..3183
                     /locus_tag="ACHURJ_004024"
                     /pseudo
     CDS             2747..3183
                     /locus_tag="ACHURJ_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608786.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          860 a          816 c          827 g          732 t
ORIGIN      
        1 aaccgagaga gaacgaatga acgccctgag aatggtcatt ccgccacgat atccctggtc
       61 tctgatggaa ggtgcgaaca agtccctgat atgagatcat gtttgtcatc tggagccatg
      121 gaacagggtt catcatgagt catcaactta ccttcgccga cagtgaattc agcagtaagc
      181 gccgtcagac cagaaaagag attttcttgt cccgcatgga gcagattctg ccatggcaaa
      241 acatggtgga agtcatcgag ccgttttacc ccaaggctgg taatggccgg cgaccttatc
      301 cgctggaaac catgctacgc attcactgca tgcagcattg gtacaacctg agcgatggcg
      361 cgatggaaga tgctctgtac gaaatcgcct ccatgcgtct gtttgcccgg ttatccctgg
      421 atagcgcctt gcctgaccgc accaccatca tgaatttccg ccacctgctg gagcagcatc
      481 aactggcccg ccaattgttc aagaccatca atcgctggct ggccgaagca ggcgtcatga
      541 tgactcaagg caccttggtc gatgccacca tcattgaggc acccagctcg accaagaaca
      601 aagagcagca acgcgatccg gagatgcatc agaccaagaa aggcaatcag tggcactttg
      661 gcatgaaggc ccacattggt gtcgatgcca agagtggcct gacccacagc ctagtcacca
      721 ccgcggccaa cgagcatgac ctcaatcagc tgggtaatct gctgcatgga gaggagcaat
      781 ttgtctcagc cgatgccggc taccaagggg cgccacagcg cgaggagctg gccgaggtgg
      841 atgtggactg gctgatcgcc gagcgccccg gcaaggtaag aaccttgaaa cagcatccac
      901 gcaagagcaa aacggccatc aacatcgaat acatgaaagc cagcatccgg gccaaggtgg
      961 agcacccatt tcgcatcatc aagcgacagt tcggcttcgt gaaagccaga tacaaggggt
     1021 tgctgaaaaa cgataaccaa ctggcgatgt tattcacgct ggccaacctg tttcgggcgg
     1081 accaaatgat acgtcagtgg gagagatctc actaaaaact ggggataacg ccttaaatgg
     1141 cgaagaaacg gtctaaatag gctgattcaa ggcatttacg ggagaaaaaa tcggctcaaa
     1201 catgaagaaa tgaaatgact gagtcagccg agaagaattt ccccgcttat tcgcaccttc
     1261 cttagcttac gttatagctg ttttcctttg cagtatttct ttgcttttct gtataccttt
     1321 atacctgtta taccagatca aaaaacaagc aatccacata acaaaacgcg ttttgttact
     1381 gatgtcacaa attgagcata tttttgtagc tgatagtttg ttacaaacac cagaaccaag
     1441 atgcctattt cgtttcagca tcaactgtta aattatctgg ctgccaacga gaatactgag
     1501 cttttttgtc cccaagactg accgtcaacg ccacaatctt tgactgtttg acattgtcat
     1561 gcccgcaggc cagcggatct aaagacatta gactgcttgc atcagccagt tgcgtatctg
     1621 gctatgtccc ttaattggta agggttcgag gaactcaaca ttaacgcatt tttcagcgca
     1681 tcaccgacag tgtatatgaa cgattgcact ctgacgatgg ttgatttatt tggttgcgta
     1741 ggggctagct agtctggtac cggaggctgg atctgaagta ctgaccccag gaataacgcc
     1801 gggctgactc atattctgac ccattgtcag cccgcgtgtt tactgctcta gctcatttcc
     1861 tgttgtcagg cgttcaattc ccaactgcag gttatggccc ggtgcacaaa cacctgacgg
     1921 caggtatcac ttacccgaag aaggtaaagc cctcaagagg cagtaactca gccaacctct
     1981 cctccggcca ctccggcaga cgcgtcagga cgtccgtcag ccaagcatgt ggctccagac
     2041 tgcggttccc agaaggctca ttatctgtgc cgcgcggttc ccggccatca gtgaaccagc
     2101 gaacaaccag gcctttcgtc ccataacgac cggacggata gcacgctcgc agatattgtt
     2161 gtgtgtgcct tgaaatgcac gatctttccg tcggtgaaag tccgccccgg ctaactctcg
     2221 ctccgactgg aaggacaaga agcggtccag gaggtaacaa aagggctgaa tctctccctg
     2281 aaacgacttc ttcaggaagt caacgggtta tgcgggccgt aacgtgagtg aacactgagc
     2341 agccatcgaa atgccgtcga gggtgctgac actccacaat aacggggaag gccgacgatg
     2401 accgggaaaa gagtgagaaa ggcatcggtc atacccaccg gggttgtggt gtcagcatgc
     2461 ataacaattg atcgatcgtg caacaagaga aatccataac gatgtctgtc cgggtgacgg
     2521 gcaacaacgc ctcgcgaggg ggtgaatcgg ttgttatgga tggcagatgg ggtgtagtag
     2581 tgaaaatact gagtaatgtc agaggagcga aggccccctc ttgcagtacc agtgtaacat
     2641 gcaggaagag cccggggatt gccatcagcc tcacaacccc gctcgaaccg gttcagaacc
     2701 tacagaaaac actacaggag aaagcaaagt gaaccgcccc gtgtttcctg tttatcattt
     2761 tctggtcagt gccgcgattc tggtattcgt ggtgattttc tggcggacac accatcgcga
     2821 ccaccgtaac tggctggctc tacggctatt cgtattatgc tctgtaaacc gttggccatt
     2881 gcggatggtt aaaggaacag ttgtggggac aactgacaca ttacgtgaaa tgcagcgtta
     2941 tgaacaactg agtcagcacg gggctgacaa ctggaaaatc cagccgggag caccgttata
     3001 tgatacgatt gttattgtca ctggtgagag tgtgcgcagg gattatatgt cagtgacata
     3061 acgtctcatc agcccgtcag tagaaaaatc agtgcccgtc atttcgcagg tatttttcag
     3121 tggatgaccg gtatttgtac tgaaaatata ccgccattca atccgctgac agacgaagat
     3181 aattaaactg ttatggtttt taagggagag aggaatatac cggcagacag tttga
//
LOCUS       DAZPEB010000280         3232 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZPEB010000280 DAZPEB010000000
VERSION     DAZPEB010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.286"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(39..665)
                     /gene="dsbA"
                     /locus_tag="ACHURJ_004025"
     CDS             complement(39..665)
                     /gene="dsbA"
                     /locus_tag="ACHURJ_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFR1282748.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(682..1668)
                     /gene="srkA"
                     /locus_tag="ACHURJ_004026"
     CDS             complement(682..1668)
                     /gene="srkA"
                     /locus_tag="ACHURJ_004026"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFR1282749.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRIVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDSTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(1745..2014)
                     /locus_tag="ACHURJ_004027"
     CDS             complement(1745..2014)
                     /locus_tag="ACHURJ_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFR1282750.1"
                     /translation="MKCKRLNEVIEFLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            2084..2668
                     /gene="mobA"
                     /locus_tag="ACHURJ_004028"
     CDS             2084..2668
                     /gene="mobA"
                     /locus_tag="ACHURJ_004028"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFR1282751.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPPDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            2650..3177
                     /gene="mobB"
                     /locus_tag="ACHURJ_004029"
     CDS             2650..3177
                     /gene="mobB"
                     /locus_tag="ACHURJ_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFR1282752.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGARHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT          847 a          786 c          772 g          827 t
ORIGIN      
        1 ccgtgaatat tcacgggctt tatgtaattt acattgaatt attttttctc ggacagatat
       61 ttcactgtat cagcatactg ctgaacaaaa acatccatat tgctggtatc cataccctgc
      121 ggattcagct gatatttacc gttaacaaac atcgccggaa caccgcgcaa ttgcacgtca
      181 gctgcagctt tttcctgctg agctaccaga gatttcacca cgaagctgtt ccacgccgcg
      241 tcgtactctt cacctttaat acctgcgttg ataaatacat cgcggatatc agaagcagaa
      301 cgaatggtct gggttttctg tacgccttca aacagcggaa cagtcacttt gtcttccacg
      361 cccagcgcca tcgccacagc ccatgcctga gtcagatctt tgcccaggtc accacccatg
      421 aagttgacgt ggtatttagt catcttcacg ccttccggca gttttttctt cacattatca
      481 gaaatatgca gaacttcttc aaactgatag cagtgcggac agaagaaaga gaaaaactcc
      541 agcacttgcg gcgcgccagc taccggtttt tccagggtag tgtactgttt accatcttca
      601 tactgcgccg ccgatgcgct aaacgctaaa actaaaccag ccagcgccag ccaaatcttt
      661 ttcatgatct actctctccg attaatacat aggtgttaat tgtaaagggg gttcttgtag
      721 aacttttgcc tgttctataa aagtcgccgt ttgtcgcagc cagtaatctt ccccggttaa
      781 ccacgggaaa tttttcggga acgcgggatc agcccaacgc cgcattagcc aggcaagata
      841 ataaaccaaa cgcatggcgc gtaaaggttc aatcagtccg atttcagcgg tgtcgaactc
      901 gctaaattct tcataagctt caataatagt ttccagttgc atccgctgct cggctttatc
      961 gccattgagc aacatccaca aatcctgaat agctggacca ttacgtgcat catccagatc
     1021 aacaaacatt ggaccatcgc gccagagaat attcccggcg tggcagtctc catgtaagcg
     1081 cagaacggtg gaatcttccc gccagtgcgc tgtaacggcg gcgatcagct catctgtcgc
     1141 tttcaggaat gccgctttca acccggaagg tatcagtgta gcgtcctcaa acagcttgcg
     1201 tggctcaatg agatattcat tcagaccgat ggtcgggcga tggataaaaa gctgtttgcg
     1261 ccccgtctgg tgcatacgcc ctaaataacg cccaaccgct tccatctgat cgatattatc
     1321 agcttcgaac tggcgaccac cgacgcttgg aaaaacagcg aaataaaatc cctgatgatt
     1381 caataaagtc tgaccgttaa aggccacagg tgctgcgacc ggaacttcat catttaccaa
     1441 ctgcaacgca aattgatgtt cttcgaggat ttgatcggct gtccaacgtt cagggcgata
     1501 aaatttgacg acaatacgtc gacgatcttc gtcctgaaat tgatagacac ggttttcata
     1561 gctgttaagc ggggtaagac cggaatccac ccggatccct tgctcaaata gagcatccat
     1621 gatggtatcc gggtgtagtg tctggaaagt aaaagcgctg ttgttcatcc catcatccgg
     1681 aaaacattac gaatgatgaa ggatatcatt tcacgatgat tttggtggcg ccgcttacaa
     1741 gcttttactc tttaattacg ccgcgagcgc gtaacagcgc cgttttgaag tcttcttcat
     1801 aatctttctg caaacccggg atcaccgcat ctttcgcgga atcacgcatt ttcaggtgat
     1861 agatcagaat gtcatccgtc aaatccgcca gttcgccgtc aaaacctgac tctttcgcca
     1921 gtttctgcaa aaattgcaac aggttaaggt ctggctcttt ttgccaggct ggctggagga
     1981 attcaataac ttcattcaga cgtttacatt tcatagtgat gctccttact cttgagacag
     2041 acacgttagc agggtcaatc ccacaataaa agaggcgata tcggtgaatc tgatgacgac
     2101 gataacaggc gttgtgctgg caggcggtaa agccagacga atgggtggcg tagataaagg
     2161 attgcttgaa ttaaacggca aaccattatg gcaacatgtc gctgacgcgc ttatgacgca
     2221 gctctctcac gtcgtggtta atgccaatcg tcatcaggaa atctatcagg caagcggtct
     2281 gaaagtgatt gaagattcac tggcggatta cccaggccct ctggcaggaa tgctttcagt
     2341 aatgcagcag gaagctggtg agtggttttt gttttgcccg tgcgatacgc cttacattcc
     2401 ccctgattta gcagcccggc ttaatcatca gcgcaaagat gcgcctgtcg tgtgggtcca
     2461 tgacggtgaa cgcgatcacc cgactattgc tctggtaaac cgcgctattg agcctttatt
     2521 actggaatat ctgcaagcag gagaacgccg ggtaatggca tttatgcgtc tggctggcgg
     2581 tcatgcggtt gatttcagcg atcataaaga tgcatttgtt aacgtgaata cgccagagga
     2641 gcttgcccga tggcaggaaa aacgatgata ccgttactcg cctttgccgc gtggagtggt
     2701 accggaaaaa ctacgctttt gaaaaaactg atcccggcat tatgcgccag agggatccgt
     2761 ccagggctga ttaagcatac gcaccatgat atggatgttg ataagccagg caaagatagc
     2821 tatgagctgc gcaaggctgg cgcggcacaa accatcgttg ccagccagca acgatgggcc
     2881 ttgatgacgg aaacaccaga cgaagaagag ctggatctac agtttctcgc aagtcgaatg
     2941 gatacctcaa agctggattt gattctggtc gaagggttta agcatgaaga gatcgcaaag
     3001 attgtgctgt ttcgcgatgg agccagacat cgacctgaag aattagtgat agacaggcat
     3061 gttattgctg tagccagtga tgtgccgctt aatcttgatg tcgcattact ggatattaat
     3121 gatgttgagg ggctggctga ttttgtggtg gagtggatgc aaaagcagaa cggataagca
     3181 agagtaggct ggatcagacg cagccagtgt cgcatccgac ctttttctgc ac
//
LOCUS       DAZPEB010000281         3224 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZPEB010000281 DAZPEB010000000
VERSION     DAZPEB010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.287"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..908
                     /locus_tag="ACHURJ_004030"
     CDS             <1..908
                     /locus_tag="ACHURJ_004030"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFR1282753.1"
                     /translation="ANIDVAGYSHESLNYADSNPGKIKFTPGGVGRNIAQNLALLGNK
                     AWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLDNTGEMLVAINDM
                     NISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVPVFVDPVSAWKCV
                     KVRDRLNQIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLNRLVLSMGGDGVY
                     YSDISGENSWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESVRFAQGCSSMALS
                     CEYTNNPDLSIANVISLVENAERLN"
     gene            875..1834
                     /gene="psuG"
                     /locus_tag="ACHURJ_004031"
     CDS             875..1834
                     /gene="psuG"
                     /locus_tag="ACHURJ_004031"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFR1282754.1"
                     /translation="MVSGERRTSELKISPELLQISPEVQDALKNKKPVVALESTIISH
                     GMPFPQNAQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEFELLGREGHNVTKVS
                     RRDLPFVVAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQEL
                     ANTNVTVVCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDS
                     ASEIARAMAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKES
                     TLFLLARVAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            1928..3177
                     /locus_tag="ACHURJ_004032"
                     /pseudo
     CDS             1928..3177
                     /locus_tag="ACHURJ_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
BASE COUNT          771 a          725 c          871 g          857 t
ORIGIN      
        1 cggcgaatat tgatgtcgcc ggatattcac atgaatcatt aaattatgcg gattcaaatc
       61 caggtaaaat aaaatttacg cctggtggag tcgggcgcaa tattgcacaa aacctggcgt
      121 tgctggggaa caaagcctgg ctactgagcg ccgtaggcag tgacttttat ggtcaatcgc
      181 tgctaacgca aaccaatcaa tctggcgttt atgtcgataa atgcctgatt gtgccgggag
      241 aaaatacgtc tagttattta tcattactcg ataataccgg tgaaatgctg gttgctataa
      301 atgacatgaa tattagcaac gctattacag ctgaatatct cgcacagcac cgtgaattta
      361 ttcagagggc aaaggtcatt gtcgcggact gtaatatcag tgaagaggca ctggcatgga
      421 ttctggataa tgccgccaac gtacccgtat ttgtcgatcc ggtttccgca tggaaatgtg
      481 tcaaagtgcg cgaccgtcta aatcagatcc acactctgaa gccaaaccgc cttgaagcgg
      541 aaaccctgag tgggattgcg ctgtcagggc gtgaagatgt ggcaaaagtt gctgcctggt
      601 tccatcaaca tggcctgaac cgactggtat tgagcatggg cggcgacggc gtttattaca
      661 gcgatatcag cggtgaaaat agctggtctg cgccgatcaa aaccaatgtt attaatgtta
      721 ccggagcggg cgatgccatg atggcgggac ttgcttcgtg ttgggtagac ggaatgccgt
      781 ttgccgaatc tgttcgtttc gcacagggat gttcgtcaat ggcgctctcc tgtgaataca
      841 ccaataaccc cgatttatcg attgccaacg ttatatcgtt agtggagaac gcagaacgtc
      901 tgaattaaaa atttcccctg aattattaca aatttccccg gaagtgcagg acgctttaaa
      961 aaacaaaaaa ccggttgtgg cgctggaatc gaccattatt tctcacggga tgccgttccc
     1021 acaaaatgcc cagaccgcaa ttgaagtaga agaaactatt cgtaaacagg gcgctgtacc
     1081 tgccactatc gccattattg gcggcgtgat gaaagtgggt ttaagcaaag aagaatttga
     1141 attactgggt cgtgaagggc ataacgtgac caaagttagt cgtcgcgatt taccttttgt
     1201 tgttgccgcc ggaaaaaatg gcgcaaccac tgtggcttca acgatgatta ttgcggcgct
     1261 tgccggaatt aaagtatttg ccaccggggg aattggtggt gtgcatcgcg gggcggaaca
     1321 taccttcgat atttctgccg atttgcaaga actggcaaat actaatgtca ccgttgtttg
     1381 tgccggggcg aaatctattc tcgatttagg attaaccact gagtatttag aaaccttcgg
     1441 tgtgccgtta attggctatc agactaaagc gctgcctgcg tttttctgcc gtaccagctc
     1501 gtttgacgtc agtattcgtc tcgacagcgc cagtgaaatt gcccgtgcaa tggcggtgaa
     1561 atggcaaagc gggctgaacg gtggcctcgt ggtagcgaac ccgatcccgg aacagtttgc
     1621 gatgccggag gaatctatca atgcagccat agatcaagcc gtcgccgaag ccgaagagca
     1681 gggcgttatt ggtaaagaaa gtacactgtt cctgctggcg cgcgttgctg agctgaccgg
     1741 cggtgacagc ctgaaatcca atatccagct ggtattcaac aacgccattc tggcgagtga
     1801 aattgccaaa gaataccagc gtctcgcggg ttaatttccg acgggcagaa aaccgtcgcc
     1861 cgtactcatc aagcatacca aaacctggcg ctgacgccgg gtttactggc atgaagggaa
     1921 aatcattatg gatataatga gaagtgttgt ggggatggtg gtgttactgg caatagcatt
     1981 tctgttgtca gtgaataaaa agagcatcag tttgcgcacg gttggagccg cactgctgct
     2041 gcaaatcgct attggtggca tcatgctcta cttcccaccg ggaaagtggg cagtagaaca
     2101 ggcggcatta ggcgttcata aagtgatgtc ttacagtgat gccggtaggc cttcattttt
     2161 ggttcgctgg ttgggccgaa aatggatgtc ctgtttgacg gtgcgggttt tatcttcgcc
     2221 tttcgcgtac ttccggcgat tattttcgtt actgcgctca tcagtctgct gtactacatt
     2281 ggcgtgatgg ggctgctgat tcgcatcctt ggcagcattt tccagaaagc tcttaacatc
     2341 agcaaaatcg aatcttttgt cgcagtcaca actattttcc tcgggcaaaa tgagatcccg
     2401 gcgatcgtta aaccgtttat cgatcgcatg aatcgcaacg agttgtttac cgcaatttgt
     2461 agcgggatgg cgtccattgc tggttcgatg atgattggtt atgccggaat gggcgtacca
     2521 attgactacc tgttagcggc atcgctgatg gcgatcccag gtggtatttt gtttgcacgt
     2581 attcttagcc cggccaccga gccttcgcag gtcacatttg aaaatctgtc gttcagcgaa
     2641 acgccgccaa aaagctttat cgaagcggcg gcgagcggtg cgatgaccgg gctgaaaatc
     2701 gccgctggtg tagcgacggt ggtaatggca tttgtcgcaa ttattgcgct gatcaacggc
     2761 attatcggcg gaattggtgg ctggtttggt ttcgccaatg cctctctgga aagtattttt
     2821 ggctatgtgc tggcaccgct ggcgtggatc atgggtgtgg actggagtga tgccaatctt
     2881 gcgggtagcc tgattgggca gaaactggcg attaacgaat tcgtcgctta cctgagtttc
     2941 tccccatacc tgcaaacggg cggcacgctg gaagtgaaaa ccattgcgat tatctccttt
     3001 gcgctttgtg gttttgctaa ctttggttct atcggtgttg tcgttggcgc attttcggct
     3061 atttcgccaa aacgcgcgcc ggaaatcgcc cagcttggtt tacgggcgct ggcagcagca
     3121 acgctttcca acctgatgag tgcgactatt gccgggttct ttattggttt agcttgagtt
     3181 tcattgccgg atgtacgcgc ttattaagcg ttgcatccgg caat
//
LOCUS       DAZPEB010000282         3210 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZPEB010000282 DAZPEB010000000
VERSION     DAZPEB010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.288"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            57..1208
                     /gene="yiaY"
                     /locus_tag="ACHURJ_004033"
     CDS             57..1208
                     /gene="yiaY"
                     /locus_tag="ACHURJ_004033"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFR1282755.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAYQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNSLKDACGFTNPIQATHEEIVAIYRAAM"
     gene            1373..2911
                     /gene="aldB"
                     /locus_tag="ACHURJ_004034"
     CDS             1373..2911
                     /gene="aldB"
                     /locus_tag="ACHURJ_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFR1282756.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
BASE COUNT          779 a          799 c          872 g          760 t
ORIGIN      
        1 cgaatgaaaa tcctcagtaa gcttcccacc cttttttaca ctttcaggag tgtgttatgg
       61 catcttcaac tttctttatt ccttctgtga atgtcatcgg cgctgattca ttgactgatg
      121 caatgaatat gatggcagat tatggattta cccgtacctt aattgtcact gacagtatgt
      181 taacgaaatt aggtatggcg ggcgatgtgc aaaaagcact ggaagaacgc aatattttta
      241 gcgttattta tgatggcacc caacctaacc ccaccacgga aaacgtcgcc gcaggtttga
      301 aattacttaa agagaataat tgcgatagcg tgatctcctt aggcggtggt tctccacacg
      361 actgcgcaaa aggtattgcg ctggtggcag ccaatggcgg cgatattcgc gattacgaag
      421 gcgttgaccg ctctgcaaaa ccgcagctgc cgatgatcgc catcaacacc actgcgggta
      481 cggcctctga aatgacccgt ttctgcatca tcactgacga agcgcgtcat atcaaaatgg
      541 cgattgttga taaacatgtc actccgctgc tttctgtcaa tgactcctct ctgatgattg
      601 gtatgccgaa gtcactgacc gccgcaacgg gtatggatgc cttaacgcac gctatcgaag
      661 catatgtttc tattgccgcc acgccgatca ctgacgcttg tgcactgaaa gccgtgacca
      721 tgattgccga aaacctgccg ttagccgttg aagatggcag taatgcgaaa gcgcgtgaag
      781 caatggctta tgcccagttc cttgccggta tggcgttcaa taatgcttct ctgggttatg
      841 ttcatgcgat ggcgtaccag ctgggcggtt tctacaacct gccacacggt gtatgtaacg
      901 ccgttctgct gccccatgtt caggtattca acagcaaagt cgccgctgca cgtctgcgtg
      961 actgtgccgc tgcaatgggc gtgaacgtga caggtaaaaa cgacgcggaa ggtgctgaag
     1021 cctgcattaa cgccatccgt gaactggcga agaaagtgga tatcccggca ggcctacgcg
     1081 acctgaacgt gaaagaagaa gatttcgcgg ttctggcgac taattccctg aaagatgcct
     1141 gtggttttac taacccgatc caggcaactc acgaagaaat tgtggcgatt tatcgcgcag
     1201 cgatgtaatc atcatttcca caacggctgg caaattgtca gccgcttttt caactatctc
     1261 tgtaaccctt gcccgtaaat tcgtgatagc cgtcgtaaag ctgttaccga ctggcgaaga
     1321 tttcgccagt cacgtctacc cttgttatac ctcacaccgc aaggagacga tcatgaccaa
     1381 taatccccct tcagcacaga ttaagcccgg cgagtatggt ttccccctca agttaaaagc
     1441 ccgctatgac aactttattg gcggcgaatg ggtagcccct gccgatggtg agtattacca
     1501 gaacctgacg ccggtgactg ggcagctgct gtgcgaagtg gcgtcttcgg gcaaacgaga
     1561 catcgatctg gcgctagatg ctgcgcacaa agtgaaagat aaatgggcgc acacctcggt
     1621 gcaggatcgc gcggcgattc tgtttaagat tgccgatcga atggaacaaa acctcgagct
     1681 gttagcgaca gctgaaacct gggataacgg caaacccatt cgcgaaacca gtgctgccga
     1741 cgtgccgctg gcgattgatc attttcgcta tttcgcctcg tgtattcggg cgcaggaagg
     1801 tgggatcagt gaagttgata gcgaaaccgt ggcctatcat ttccatgaac cgttaggcgt
     1861 ggtggggcag attatcccgt ggaacttccc gctgctgatg gcgagctgga aaatggctcc
     1921 cgcgctggcg gcgggcaact gtgtggtgct gaaacccgca cgtcttaccc cgctttctgt
     1981 actgctgcta atggaaattg tcggtgattt actgccgccg ggcgtggtga acgtggtcaa
     2041 tggcgcaggt ggggaaattg gcgaatatct ggcgacctcg aaacgcatcg ccaaagtggc
     2101 gtttaccggc tcaacggaag tgggccaaca aattatgcaa tacgcaacgc aaaacattat
     2161 tccggtgacg ctggagttgg gcggtaagtc gccaaatatc ttctttgctg atgtgatgga
     2221 tgaagaagat gcctttttcg ataaagcgct ggaaggcttt gcactgtttg cctttaacca
     2281 gggcgaagtt tgcacctgtc cgagtcgtgc tttagtgcag gaatctatct acgaacgctt
     2341 tatggaacgc gccatccgcc gtgtcgaaag cattcgtagc ggtaacccgc tcgacagcgt
     2401 gacgcaaatg ggcgcgcagg tttctcacgg gcaactggaa accatcctca actacattga
     2461 tatcggtaaa aaagagggcg ctgacgtgct cacaggcggg cggcgcaagc tgctggaagg
     2521 tgaactgaaa gacggctact acctcgaacc gacgattctg tttggtcaga acaatatgcg
     2581 ggtgttccag gaggagattt ttggcccggt gctggcggtg accaccttca aaacgatgga
     2641 agaagcgctg gagctggcga acgatacgca atatggcctg ggcgcgggcg tctggagccg
     2701 caacggtaat ctggcctata agatggggcg cggcatacag gctgggcgcg tgtggaccaa
     2761 ctgttatcac gcttacccgg cacatgcggc gtttggtggc tacaaacaat caggtatcgg
     2821 tcgcgaaacc cacaagatga tgctggagca ttaccagcaa accaagtgcc tgctggtgag
     2881 ctactcggat aaaccgttgg ggctgttctg atataagaag ctggtcgcat tgggtattca
     2941 ttgcctgatg tgacgcttac gcgtcttatc atgcctacgg gaacctgacc gtaggccgga
     3001 taaggcgtac acgccgcatc cggcataaac aaagcgcacg ttgttacaat ttacgtttta
     3061 aacctacgtg cgcaataatc cagactacgc caacagaagt gcgtttctgg ggcgtgtttt
     3121 gtcaggagtt taatctcgcg tttcgatggt gagcgggcga actaggccgt aaatcaccat
     3181 tggtgatccg aggccaatca gcaataaacg
//
LOCUS       DAZPEB010000283         3207 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZPEB010000283 DAZPEB010000000
VERSION     DAZPEB010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.289"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..923)
                     /gene="curA"
                     /locus_tag="ACHURJ_004035"
     CDS             complement(<1..923)
                     /gene="curA"
                     /locus_tag="ACHURJ_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFR1282757.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSHVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGGAGGAEKCRHAIEVLGFDVCLDHHAGDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDK"
     gene            1104..1622
                     /gene="mddA"
                     /locus_tag="ACHURJ_004036"
     CDS             1104..1622
                     /gene="mddA"
                     /locus_tag="ACHURJ_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFR1282758.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDSGKHVMVAGIESQNQASLHLHHSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            1619..2068
                     /locus_tag="ACHURJ_004037"
     CDS             1619..2068
                     /locus_tag="ACHURJ_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR1282759.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(2069..2302)
                     /gene="ydcY"
                     /locus_tag="ACHURJ_004038"
     CDS             complement(2069..2302)
                     /gene="ydcY"
                     /locus_tag="ACHURJ_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFR1282760.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(2388..2561)
                     /gene="ortT"
                     /locus_tag="ACHURJ_004039"
     CDS             complement(2388..2561)
                     /gene="ortT"
                     /locus_tag="ACHURJ_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFR1282761.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            2756..2851
                     /gene="yncL"
                     /locus_tag="ACHURJ_004040"
     CDS             2756..2851
                     /gene="yncL"
                     /locus_tag="ACHURJ_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFR1282762.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
BASE COUNT          794 a          813 c          747 g          853 t
ORIGIN      
        1 attttatcct ctttcaccca ttgccccatc tccttctgaa actcatggat gcggtgacca
       61 taatcctgag cgataataaa cccttgcaag cgaatacgtt ttttcagcac cgttgccatc
      121 aacaggggta aacgatccgg accgggtggt agctctgtag cgttatagct gctcactaag
      181 ccacaaacgg gaatgcgcgc agatgtatta agtaatggta gcactgcatc aaatacctta
      241 ccgcccacgt tttcataata gatatcaata ccttttgggc acgctttcac cagttgttcg
      301 gcaaaatcac ccgcgtggtg atcaagacaa acatcgaagc ccagcacctc gatagcatgg
      361 cggcattttt ccgcgccacc ggcccccccc accaccctgc aacctttaag tttgccgatt
      421 tgccccaccg ttgctcccac aggacctgtc gccgcagcta ccaccaacgt ttcgccctct
      481 ttaggctgac cgatatccag cagccccata taagcggtaa agcctggcat ccccagcaca
      541 cccagcgacc acgatggatt ttgcggatga tcgccaagtt tcaccagatc atcaccactg
      601 gatatgtcat agtcctgcca tccactgtag cccagcaccc agtcgccagg ctgataatca
      661 ggatgattcg actccacaac atggctcacc gtgccgccga ccatcacgcc gccaatatca
      721 acaggcggtg aataagatgg ctcatcgctc atacgtccac gcatatacgg gtccagggac
      781 aaataaactg tgcgcagtaa cacctgtcct tcacccggtg tggcgacatc atcttcttca
      841 agacggaaat tctccggaac cggtgcgcca tgtggacgcg aggccagaac ccaacgtcga
      901 ttacgctgct tttgttgccc cataatgctc tcctgtgctt ttggcatgaa tccatcaata
      961 gtagcaggct cacgggattg gttttgtttg cagccgtcac atcccctctg caacacgcaa
     1021 taatttcatc gaggaatccg ttgcttcggg ctgttttcga tagtattgat aacatcatca
     1081 tcattatttt atcgaggcca gacatgtcca tccgttttgc ccgcaaagcc gattgtgctg
     1141 ccattgcgga aatttataac cacgccgtgt tgtatacggc ggctatctgg aatgaccaaa
     1201 cggttgatgc agataaccgc attgcctggt ttgaagcgcg gaccatagca ggttatccgg
     1261 tgctggtaag tgaagaagat ggcgtagtga cgggatatgc ctcgtttggc gactggcgta
     1321 gtttcgatgg ttttcgccat accgtggaac attcggttta tgtccatccc gatcatcagg
     1381 gcaaaggtct ggggcgtaaa ttgttaagcc gattgattga tgaagcgcgg gatagcggga
     1441 agcatgtcat ggtcgccggg atcgaatcgc aaaatcaggc ctcgctgcat ctgcaccatt
     1501 cgctgggatt tgtcgtcacc gcacaaatgc cgcaggtggg cactaaattt gggcgctggc
     1561 tggatctgac atttatgcag ttgcaactcg acgaacgcac tgaaccggac gcgattggat
     1621 gaatcagtcg ctcacccttg cttttttgat tgccgccgga attggtctgg tagtccagaa
     1681 cacgctgatg gtgcgcatca cccagacctc ctctaccatt ctcatcgcca tgttgctgaa
     1741 ctcactggtg gggattgtgc tctttgtttc tattttatgg tttaaacaag gcatggcggg
     1801 gtttggcgaa ctggtgtcca gcgtgcgctg gtggacactt atacccggct tgctgggatc
     1861 gttttttgtc ttcgccagta tcagcggata tcagaatgtg ggagcggcga caactattgc
     1921 agtactggtc gccagccagc taattggcgg tctgatgctg gatatcttcc gcagtcatgg
     1981 cgtaccacta cgggcgttgt tcggcccaat ttgtggcgca attttacttg tggttggcgc
     2041 ctggttggta gccagacgct cattttaatc agaggatggt gccgcctttg gttaactgtt
     2101 cgtggcgcgc ttccatatct tctttatgct tgcgaccgtg atgagcaata gcggtgcgca
     2161 aacgctgctg ctgggtgtag cgatcttccc gacttaactc cgcgtcatcg ctcagggcga
     2221 ttaataattc gttcatatgg gcaatgacgc tctcttcaat cgcggcatcc acgcgcgcga
     2281 tgacttcatc cagatgcgac atactttctc cttaaccact tgtgaaattt atcgattcat
     2341 ccaaatgata taagtctgcg tggtgccgtc aagccagtga gtaagattta aaacagtgaa
     2401 aacaacagcg ccaccgggaa actcatcggc catgttgccc ccaccagaaa ggcgcttaag
     2461 aaacggatgc gtttcttatc gtgagaaaga aaccaggtga taagaaatgc gattgctgcc
     2521 ataaccgcat aaaaaacaag catatgttga tagagagaca tgtacgggcg tccagccaga
     2581 tgaataaaac gcgcgcaaat atgcgtcatt ttttgacgtg catcaagttt tttcaccagt
     2641 ttgagaaaaa atcatcaaac agcaacttgc ccttatccat cttcagaatg agtattattg
     2701 cggattttct taacccgtac tatacacata ggggcaatga taaaaggtgg caaaaatgaa
     2761 tgtttccagt agaactgtag tactgataaa tgtctttgct gctgttggtt tgtttactct
     2821 tatctctatg agatttggct ggtttatttg atgtagtgat tttgcacgca gcacgtactg
     2881 tcagagaaat caactctctg acagtacgca agatcttctc atactctcca ggtttacaac
     2941 attatcctta atatttactt ctttttgctg gcatctacat tgaaatgagt agaaaacatc
     3001 cccctactgt cgtctataca aacagcttac tgattcaaac ggaatcttgt tttcacattt
     3061 ttctatcatg aatactttat acgcgcggga gtctataaat gctgaggtag cctgagttta
     3121 acggacactc cttcctgaaa tagaatggca tcagaaggag ctaataatga gcagaaaaaa
     3181 ccaacgttac tctaaagagt tcaaagc
//
LOCUS       DAZPEB010000284         3200 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZPEB010000284 DAZPEB010000000
VERSION     DAZPEB010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.290"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            199..939
                     /gene="dpaA"
                     /locus_tag="ACHURJ_004041"
     CDS             199..939
                     /gene="dpaA"
                     /locus_tag="ACHURJ_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFR1282763.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(910..1677)
                     /locus_tag="ACHURJ_004042"
     CDS             complement(910..1677)
                     /locus_tag="ACHURJ_004042"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFR1282764.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(1883..2461)
                     /gene="lpcA"
                     /locus_tag="ACHURJ_004043"
     CDS             complement(1883..2461)
                     /gene="lpcA"
                     /locus_tag="ACHURJ_004043"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFR1282765.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            2701..>3200
                     /gene="fadE"
                     /locus_tag="ACHURJ_004044"
     CDS             2701..>3200
                     /gene="fadE"
                     /locus_tag="ACHURJ_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="functions in fatty acid oxidation; converts
                     acyl-CoA and FAD to FADH2 and delta2-enoyl-CoA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFR1282766.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFA"
BASE COUNT          767 a          811 c          820 g          802 t
ORIGIN      
        1 atcttaggaa ataaatgata catgcgcttt tcggactatt gaaaactgcc cctgtgctaa
       61 atttgtaaac cctgcgccga caggcttgcg taaaactctt ttggtgataa tgtgagcctt
      121 ccaaatccag actattccga tttgggatcc attttgccgt cctggcgtgt gtaaccgttt
      181 tatcaaggaa taagcagtat gcgtaaaatc gcattaattc ttgcgatgtt gttgatcccg
      241 tgcgtttcgt ttgccggttt gctgggcagc agtagctcga ccacgccagt gagcaaagag
      301 tataagcagc agttgatggg atcccctgtc tacatccaga tcttcaagga agaacgtacg
      361 ctcgatctct acgtcaaaat gggcgagcaa tatcagctgc tcgacagtta taaaatctgt
      421 aaatattccg gcggcttagg gccaaaacag cgtcagggcg atttcaaaag cccggaaggg
      481 ttttatagcg tccagcgtaa tcagttaaaa ccagacagcc gttactacaa agcgattaat
      541 attggtttcc ccaatgccta tgaccgtgcg catggttatg aagggaaata cctgatgatc
      601 cacggcgatt gtgtttccat cggctgctac gcaatgacca atcagggtat tgatgagatc
      661 ttccagttcg ttactggtgc gctggtgttt ggtcagccga gcgtgcaagt gagtatttac
      721 ccgttccgca tgaccgacgc caatatgaag cgccataaat attccaactt taaggacttc
      781 tgggagcaac tgaagccggg ttacgactac tttgagcaaa cccgtaagcc accgaccgtt
      841 tctgtggtca atggtcgtta cgtggtcagc aagccgttaa gccacgaagt tgtgcagcca
      901 caactggcat caaactacac gctccccgag gcaaaataag cgccattcgc ctggcataat
      961 cttttgccag gtttcattgc ccgtcagcgg ctgtgtcgca atcaccgtga ccacatcatt
     1021 cggtgtggtc tgcgagctga agtcgatttc tacatcctga tccagcagcg ttgccacgcc
     1081 aaacggtgcg cggcgggtga tccagtgcag attcgtcgag caatacgcca ttacatagcg
     1141 cccatccgaa agcagcatgt tgaaaacgcc cttctgccgc agttcgtccg ccagtgaagc
     1201 gatatattta aacactgctg ccatgttgcc cggcgtgcgc gggtaacgct gcgttaattt
     1261 atgcagcagc cagcagaagg ctttttcgct gtcggtttcg cctaccgggc ggaagttgcc
     1321 ggtttccagt gatttgtagc ccgtcagttg tccgttatgg gcataagtcc agttacgccc
     1381 ccataactcg cgggtaaatg ggtgagtatt ttccaacgcc acctcgcccc gattagcctg
     1441 gcgaatatga gccaccaccg aacaggattt tatcgggtag tcctggacaa gtttggcgat
     1501 gggggaatta aagctgggtt gtggatcttt aaatgtgcga cagcctttac cttcgtaaaa
     1561 ggtaatgccc cagccatctt tatgtggccc ggttccacca ccacgctgta caagtccggt
     1621 gaaactaaag cagatatcgg tagggacgtt ggcgctcatc ccgagcaatt cgcacatggg
     1681 taacctccac aacagcggga gcgcatggcc cccggcgaag attgatttaa gaattttcag
     1741 gtcggatgcg gcgtaaacga attattcggc ctacggttcg cgtacagatt tgtaggcatg
     1801 ataagacgcg tcagcatcgc atcaggcatc agcgcacaag tgccggatgc ggcgtaaacg
     1861 ccttatccgg cctacgccag acttacttaa ccatctcttt ttcaattaac tggatcagga
     1921 tatggatcac tttaatgtga atctcctgaa tgcggtcggc ataaccaaag tgcggtacgc
     1981 gaatttcgat atccgccgtg ccagccattt tgccgccgtc tttaccggtc agggtgatca
     2041 ctttcatccc cttctcacgc gccgctgcga tcgctttgat cacgtttgca gagttaccgg
     2101 aggtggagat ccccagcagt acatcgcctt cgcgacctac cgcttcaacg tagcgggaga
     2161 aaatatcatt gaaaccgaaa tcattaccga cacaggaaat atgactaacg tcagaaatag
     2221 caatcgccgg gtagcccgga cggttttcac ggtagcgacc ggtcaactct tcggcaaagt
     2281 gcatagcgtc gcaatgggaa ccgccgttgc cgcaggaaag cactttgcca ccggctttaa
     2341 agctgtctgc taacaggacc gccgcgcgct gaatggcgtg aatattggcg tcatctttta
     2401 aaaagttagc cagcgtttcc gccgcttcgt tcagttcgtt acgaataaga tcttggtaca
     2461 tgaggatatc cttcagcata aatgtaatag acaaaatgca gtgtaccgga taccgccaaa
     2521 agcgagaagt acgggcaggt gctatgacca ggactttttg acctgaagtg cggataaaaa
     2581 cagcaacaat gtgagctttg ttgtaattat attgtaaata cattgctaaa tgtatttaca
     2641 tccactacaa ccatatcatc acaagtggtc agacctccta caagtaaggg gcttttcgtt
     2701 atgatgattt tgagtattct cgctacggtt gtcctgctcg gcgcgttgtt ctatcaccgc
     2761 gtgagcttat ttatcagcag tctgattttg ctcgcctgga cagccgccct cggcgttgct
     2821 ggtctgtggt cggcgtgggt actggtgcct ctggccatta tcctcgtgcc atttaacttt
     2881 gcgcctatgc gtaagtcgat gatttccgcg ccggtatttc gaggtttccg taaggtgatg
     2941 ccgccgatgt cgcgcactga gaaagaagcg attgatgcgg gcaccacctg gtgggagggc
     3001 gacttgttcc agggcaagcc ggactggaaa aagctgcata actatccgca gccgcgcctg
     3061 accgccgaag agcaagcgtt tctcgacggc ccggtagaag aagcctgccg gatggcgaat
     3121 gatttccaga ttactcatga gctggcggat ctgccgccgg agttgtgggc gtaccttaaa
     3181 gagcatcgct tcttcgcgat
//
LOCUS       DAZPEB010000285         3175 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZPEB010000285 DAZPEB010000000
VERSION     DAZPEB010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.291"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            357..507
                     /locus_tag="ACHURJ_004045"
                     /pseudo
     CDS             357..507
                     /locus_tag="ACHURJ_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(725..2815)
                     /gene="iha"
                     /locus_tag="ACHURJ_004046"
     CDS             complement(725..2815)
                     /gene="iha"
                     /locus_tag="ACHURJ_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HFR1282767.1"
                     /translation="MRITTLASVVIPCLGFSASSIAAAEDVMIVSASGYEKKLTNAAA
                     SVSVISQEELQSSQYHDLAEALRSVEGVDVESGTGKTGGLEISIRGMPASYTLILIDG
                     VRQGGSSDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITRKN
                     ADKWLSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSVSLQVRGSTQQRQGSSVTSL
                     SDTAATRIPYPTESQNYNLGARLDWKASEQDVLWFDMDTTRQRYDNRDGQLGSLTGGY
                     DRTLRYERNKISAGYDHTFTFGTWKSYLNWNETENKGRELVRSVLKRDKWGLAGQPRE
                     LKESNLILNSLLLTPLGESHLVTVGGEFQSSSMKDGVVLASTGETFRQKSWSVFAEDE
                     WHLTDALALTAGSRYEHHEQFGGHFSPRAYLVWDVADAWTLKGGVTTGYKAPRMGQLH
                     KGISGVSGQGKTNLLGNPDLKPEESVSYEAGVYYDNPAGLNANVTGFMTDFSNKIVSY
                     SINDNTNSYVNSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTRSEQRDGDNKGAPLSY
                     TPEHMVNAKLNWQITEEVASWLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAW
                     TVVDAGLSWKMTDALTLNAAVNNLLNKDYSDVSLYSAGKSTLYAGDYFQTGSSTTGYV
                     IPERNYWMSLNYQF"
     gene            2754..2936
                     /locus_tag="ACHURJ_004047"
     CDS             2754..2936
                     /locus_tag="ACHURJ_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282768.1"
                     /translation="MLLAENPRQGMTTEARVVIRILLISDHVNRQDNTQIQTIMRIIY
                     ICLLQKIKIINKTKYK"
BASE COUNT          795 a          837 c          732 g          811 t
ORIGIN      
        1 tctgacctct cagtaaatat caatacggtt ctggcgagcc gcttacgtgc agcagtctta
       61 tccggcagtt taaaggggag gacggagacc aatgaggcac tggagcagat tcagtgcaga
      121 ggtgacctgc tttatcaaac ttaattgtga caaaatggcg attgagaagg tgcaattgac
      181 ttatccggat tcggtcgtgg cgaggtggtg cccctgcatt ctgctaaccg ggttatgagc
      241 caggagtcag cgatagacaa agggacttat ctgaccgggc ccccggaaaa atggcgggag
      301 agaatgatac cttcctgctg acgagaccgg actttgagat gacgagcgat agtgcggcag
      361 tgatgtacag cctgatcggt agctgcaaaa cttaacggaa tcgagccgga aacgtggcta
      421 cgccacgtga tcagtgtaat caacacctgg cctgccaacc gcatgaaaga gttgttgccc
      481 tggaacgtca ctctctctgt aaactaattt ttaccccacg tccttcacgg tgcgcttaca
      541 ctctggacaa gtatgtaaat gataagttaa aaataatccg tgcatttcct gtgacgatat
      601 tagttgttgg taacgaaatc tcattagcgg atcggaaatg cacttaacct aatacgttgc
      661 cactgttccg ccaggagttg cagtggcaac gtattctacc gtcagtgata gcgttttgtt
      721 attatcagaa ctgatagttc agcgacatcc agtaatttcg ctcaggtatc acatatcctg
      781 ttgttgatga tcccgtctgg aagtaatcac cggcatacag cgtactctta ccggcactgt
      841 acaggctcac gtcactgtaa tccttgttga gcaggttatt caccgcagca ttcagcgtca
      901 gggcatccgt catcttccag gacagacctg catccaccac cgtccaggct ttcaggtatt
      961 ctcctttctc atcatacact ttcttctgta cagcgctcag tgacgaataa ttctgggtga
     1021 aacgtggtgt tttcccgcga taacgggcac ccagccatga tgccacctct tcggtgatct
     1081 gccagttcag tttcgcattc accatgtgtt caggggtata actcagcggc gcacctttgt
     1141 tatcaccatc acgttgttca cttcgggtcc aggtgtaatt cagtgacagc gtgacatcct
     1201 ctgaccacag cggcaatgtg ccggcaaatt ccacaccgtg caaccgggcc tttccgctgt
     1261 ttacatagct attggtgtta tcatttatgg aataagagac aatcttgttg gagaagtcag
     1321 tcataaaacc tgtgacattg gcattcagac cggcggggtt atcgtaatac accccagcct
     1381 cataactgac gctctcttcc ggcttcaggt cggggttacc aagtagattt gtttttccct
     1441 gcccggacac accactaatc cctttatgta gctgccccat tctgggtgcc ttatatcccg
     1501 tggtcacacc gcctttcagc gtccaggcat ctgccacatc ccagaccaga tatgcacgcg
     1561 gactgaagtg tcccccgaat tgctcatgat gttcatagcg gctgcccgca gtcagcgcaa
     1621 gtgcatccgt gagatgccac tcatcctcag caaataccga ccagcttttc tgccggaaag
     1681 tttcacctgt gctggcaagg acaactccgt ctttcatgga cgagctctga aactcgcccc
     1741 ccaccgtaac cagatgagat tctcccagag gggtaagcag taatgaattc aggataaggt
     1801 tcgattcctt aagctcccgc ggctgaccgg caagccccca tttgtcgcgc ttcagtacac
     1861 tgcgtacgag ctcacgacct ttattttctg tctcgttcca gttcagatac gatttccatg
     1921 ttccgaaggt gaaagtatga tcatagccag ctgaaatttt gttccgctca tagcgcaggg
     1981 tccggtcata tccccccgtc agactcccca gttgcccatc ccggttatca taacgctgcc
     2041 gggtggtatc catatcaaac cagagcacat cctgctccga cgccttccag tcaagacgtg
     2101 caccaagatt ataattctgt gactccgtgg gataaggaat acgcgtggct gctgtatcgc
     2161 tcagtgatgt gaccgatgag ccctgacgct gttgtgtgct accgcgtacc tgcaggctga
     2221 cagaatcatc cacaaggggg ccactgctcc agaaattaaa ctggctgctg ttaccccatt
     2281 tgttgctttc ctgcagattc agccctgcat tgacggaaga gagccatttg tctgcattct
     2341 ttctggtaat gatattcacc acaccgccca tcgcatcaga gccatacagt gtggacatcg
     2401 gccccctgat aacctcaata cgctcaatgg cggccagagg gggcatgaac ccggtattca
     2461 tggcagaaaa accgttggga gtcacgtcac tgcttccgcc ctgacgaaca ccatcaatca
     2521 gtatcagcgt gtaactggct ggcattcctc ggatgctgat ttccagccca ccggttttac
     2581 ccgtaccact ttcaacatcc acaccctcta ctgatctcag agcctccgcc agatcgtggt
     2641 actggctgga ctgcaattcc tcctggctaa tcacagaaac actggcggct gcgttagtca
     2701 gctttttctc atagccggat gccgagacaa tcatcacatc ctctgcagca gctatgctgc
     2761 tggctgaaaa tccgagacag ggaatgacta cggaagccag agtggttatt cgcatattac
     2821 taatctccga tcatgttaac cggcaagaca atacacaaat acaaacaata atgagaataa
     2881 tttatatttg ccttttacaa aaaataaaga ttattaacaa aactaaatat aaataacaac
     2941 tgcctcggca tgccagttaa cagctatccg tatggcgtgt tcggttttgc gaataaaaaa
     3001 tggttactgt agaagcggat ttccaccaca tgtcaaccat gaatggtcag tggtgcgaga
     3061 cgactgggat gatttggtcg atgacttctt ccggtgaata ctgatagaaa cattgaaata
     3121 ttgtaagaga aagcgttcaa aattctgtgg cacgtgaaaa attctacagc gtcat
//
LOCUS       DAZPEB010000286         3156 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZPEB010000286 DAZPEB010000000
VERSION     DAZPEB010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.292"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..398)
                     /locus_tag="ACHURJ_004048"
     CDS             complement(<1..398)
                     /locus_tag="ACHURJ_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR1282769.1"
                     /translation="MSVVISGALTDGAGIPMSGCQIILKSRVNTSEVVMRTRADVVTG
                     NNGEYSFEAQVGKYCVYLKRDWRDEYCVGDIAVYDDSKPGTLNDFLTAPDEGDLKPDV
                     VKRFEEMVAQAQQSAGAAAGNAQQTAQDVAA"
     gene            complement(422..967)
                     /locus_tag="ACHURJ_004049"
     CDS             complement(422..967)
                     /locus_tag="ACHURJ_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR1282770.1"
                     /translation="MLHLKNITAGNPKTAEQYQLTKQYDVTWLFSEDGKNWYEEQKNF
                     ASDTIKMVYTGDGRVVWVGKDVTGIEPRNASVIEVPDITANRRITAPGYWFYRNDEFV
                     FDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISDEDREKLKAYRIYAKSLQAMDFS
                     AITDKSSYNAIEWPVSPEGSS"
     gene            complement(970..1491)
                     /locus_tag="ACHURJ_004050"
     CDS             complement(970..1491)
                     /locus_tag="ACHURJ_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282771.1"
                     /translation="MDVYANGQHVFRFQNGVAIALKNIQAGNAKKFTLSSANNSTKNA
                     AFYLWGNPSRPVVAELGDDSGWHFYSQRNTDGSITFAVNGQVIPLNYGNFDARYKHRT
                     EGVQDVRYGYEMYYNPGSNTVSWTFRSPSGHGLSGISISDTGRNSADNVNGVYYRPLQ
                     KLINGIWYNVASI"
     gene            complement(1626..2177)
                     /locus_tag="ACHURJ_004051"
                     /pseudo
     CDS             complement(1626..2177)
                     /locus_tag="ACHURJ_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554725.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            complement(2164..2754)
                     /locus_tag="ACHURJ_004052"
     CDS             complement(2164..2754)
                     /locus_tag="ACHURJ_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HFR1282772.1"
                     /translation="MDSLQDDYTKLLYGLMQPGPAWSDTDGVLDGLAPSLVRVHQRAD
                     ELVIEIDPGQSTELIERYEELYGLPDSCSPVGTQTLRQRQQRLEAKANVAGGINEQFF
                     LDQLEALGYTGVTIEQFQHLDASPDPEWGDRWRYFWRVTLPVDAGAQWQTCTDACNTP
                     IRTWGDTVAECVINKLCPSHTVVLFSYPDEDEDAQD"
     gene            complement(2754..2912)
                     /locus_tag="ACHURJ_004053"
     CDS             complement(2754..2912)
                     /locus_tag="ACHURJ_004053"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282773.1"
                     /translation="MYLSRISEAISLSAGEVAHRLIVPSSDIDLGETELPVLGEITWQ
                     AYDPARSK"
     gene            2994..>3156
                     /locus_tag="ACHURJ_004054"
     CDS             2994..>3156
                     /locus_tag="ACHURJ_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR1282774.1"
                     /translation="MSRKNQRYSKEFKAETVRTVLENQLSISEGASRLSLPEGTLGQW
                     VTAARKGLGT"
BASE COUNT          761 a          885 c          696 g          814 t
ORIGIN      
        1 gccgccacat cctgcgccgt ctgctgtgcg tttccggctg cggccccggc gctctgctgc
       61 gcctgcgcca ccatttcctc aaagcgtttg acgacatccg gcttcaggtc gccttcatcg
      121 ggggcagtca gaaagtcatt cagcgtgccg ggctttgagt cgtcgtatac agcaatgtcg
      181 ccaacacagt actcgtcgcg ccagtcccgt ttcagataca cgcaatattt tccgacctgt
      241 gcctcaaacg aatattcacc gttatttccg gtcactacat cagccctggt acgcatcacc
      301 acttctgagg tgtttacacg ggatttcaga ataatctggc atccggacat ggggatgcct
      361 gcgccatcag tcagcgcacc tgatatcact acagacatag tttttctcgc gataaattaa
      421 atcaggaaga accttccgga gagacgggcc attcaatggc gttgtatgag gatttatcag
      481 tgatggcgct gaaatccatc gcctgcagcg atttcgcgta aatacggtac gctttcagtt
      541 tttcccggtc ttcgtcgctg attagcccca gcagcaggtc ttcttcccat tcccctgtcc
      601 gggcactgac ctgagccaga agggcatcac gctcatcttc cgctttgagt ctgtagtcaa
      661 agacaaattc atcattgcgg taaaaccagt aacccggcgc ggtaatgcgg cggttggcgg
      721 taatatcagg aacttcaata acactggcgt tacggggttc aatgcctgtc acatccttac
      781 cgacccacac cacgcgcccg tctccggtgt aaaccatttt tatggtgtca ctggcaaagt
      841 tcttctgttc ttcataccag tttttgccgt cttccgaaaa aagccaggtg acatcatact
      901 gttttgtcag ctgatattgt tccgcggttt tcggattacc tgccgtaata ttttttaaat
      961 gcaacattgt taaatactcg ctacgttata ccagatgcca ttaatcagtt tttgcagcgg
     1021 tcggtaatac acaccgttga cgttatccgc tgagttacgg ccggtatccg atatcgatat
     1081 ccctgacaac ccgtgtcccg aaggtgagcg aaacgtccat gaaacggtgt tgctgccagg
     1141 gttgtaatac atttcataac cataccgcac atcctgtacc ccctcagttc gatgtttata
     1201 gcgggcatcg aagtttccgt aatttaacgg aattacctgt ccgtttacag cgaatgtgat
     1261 actaccatcg gtatttctct ggctgtagaa atgccagccg gaatcatcac caagctctgc
     1321 aacaacaggc ctggatggat taccccacaa ataaaacgct gcatttttcg tggagttgtt
     1381 ggcgctggat aacgtgaatt ttttagcatt tccggcctga atatttttta acgctatcgc
     1441 cacaccattc tggaaacgaa atacatgctg tccattcgca taaacatcca gaatgccgtc
     1501 gccgttttgt tttatacctg tatcgttatc cccgaaagca attgaattcc ccccagggcg
     1561 ttctgaacgc cgatacccag cgcaccattg acctgcgaac caccgccaac agacacttta
     1621 tgcgacatgg atatttcacc cgtccgcaga ttaatggtga acgggcgcag gggaccaata
     1681 tcgccatttt ccccctgacc ttcactggtg ggaataaggt gcaggcactc ttccgaacga
     1741 cgaaaaatca ggccaaaagc ttcgttgaaa atcctcagcg cattaacacc acggattttc
     1801 agctctccgg tcatggtgtc gccatcacgc tgaacggcat tttttgcctt atccaccgtg
     1861 ggttttaatc cgaggttttc aacagcctca tccctgtctt ccacatccga aaggttattt
     1921 tttatcagca atgcctcttc gttaattgca ccgcccacca gcaataatat agccttatat
     1981 agctggtcgt gttcttcttt attcagttct atcccggcct tctcaatgac accacagatt
     2041 tcctcctgaa tggcattcca catggcactg ttcagccagg tagcaagacg tcctgtgcga
     2101 agatttccgt cagtaaatcc gttcttaccc ggaccaaact tatcttttac cgcagtcagc
     2161 gtatcaatcc tgtgcatctt catcctcatc tggataggaa aataaaacga cggtgtgtga
     2221 cggacataat ttattaatca cgcactccgc aaccgtatcc ccccacgtcc ggatcggtgt
     2281 gttgcaggcg tccgtgcatg tctgccactg agcaccggca tccaccggca acgtcacacg
     2341 ccagaaataa cgccagcgat ctccccattc cggatcgggg cttgcatcca ggtgctggaa
     2401 ctgttcgatc gttacgccgg tatatcccag cgcctcaagc tgatccagga agaactgctc
     2461 atttatgcca ccagccacat tggcttttgc ttcaagacgt tgctgacgct ggcgtaatgt
     2521 ctgggttccg acaggggaac aggaatcagg taaaccatac aattcttcat aacgttcaat
     2581 cagctctgtg gactgaccgg gatcgatttc aatcaccagt tcatcagccc gctggtgaac
     2641 acgcacaagc gatggtgcca gaccgtcaag tacaccgtcg gtatctgacc atgcaggccc
     2701 cggctgcatc agtccgtaca gcagctttgt ataatcatcc tgtaacgaat ccattattta
     2761 ctccttgccg ggtcataagc ctgccaggtg atctcgccga gcaccggaag ctcggtctcc
     2821 cccaggtcaa tatccgatga agggacgatt aaccggtggg ccacttcacc agcagacaaa
     2881 ctgatggcct cactgattct ggacagatac atacgcccct ctggcacact gaggtagcct
     2941 gagtttaacg gacactcctt cctgaaatag aatggcatca gaaggagcta ataatgagca
     3001 gaaaaaacca acgttactct aaagagttca aagccgaaac tgtcagaacg gttcttgaaa
     3061 atcaactttc gatcagtgaa ggcgcttccc gattatccct tcctgaaggc actttaggac
     3121 aatgggttac cgccgccaga aaagggctcg gtactc
//
LOCUS       DAZPEB010000287         3114 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZPEB010000287 DAZPEB010000000
VERSION     DAZPEB010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.293"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            80..2983
                     /locus_tag="ACHURJ_004055"
     rRNA            80..2983
                     /locus_tag="ACHURJ_004055"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          827 a          681 c          965 g          641 t
ORIGIN      
        1 gaacaacgaa agttgttcgt gagtctctca aattttcgca acacgatgat gaatcgtaag
       61 aaacatcttc gggttgtgag gttaagcgac taagcgtaca cggtggatgc cctggcagtc
      121 agaggcgatg aaggacgtgc taatctgcga taagcgtcgg taaggtgata tgaaccatta
      181 taaccggcga tttccgaatg gggaaaccca gtgtgattcg tcacactatc attaactgaa
      241 tccataggtt aatgaggcga accgggggaa ctgaaacatc taagtacccc gaggaaaaga
      301 aatcaaccga gattccccca gtagcggcga gcgaacgggg agcagcccag agcctgaatc
      361 agtgtgtgtg ttagtggaag cgtctggaaa ggcgtgcgat acagggtgac agccccgtac
      421 acaaaaatgc acatattgtg agctcgatga gtagggcggg acacgtggta tcctgtctga
      481 atatgggggg accatcctcc aaggctaaat actcctgact gaccgatagt gaaccagtac
      541 cgtgagggaa aggcgaaaag aaccccggcg aggggagtga aaaagaacct gaaaccgtgt
      601 acgtacaagc agtgggagca cgcttaggcg tgtgactgcg taccttttgt ataatgggtc
      661 agcgacttat attctgtagc aaggttaacc gaatagggga gccgaaggga aaccgagtct
      721 taactgggcg ttaagttgca gggtatagac ccgaaacccg gtgatctagc catgggcagg
      781 ttgaaggttg ggtaacacta actggaggac cgaaccgact aatgttgaaa aattagcgga
      841 tgacttgtgg ctgggggtga aaggccaatc aaaccgggag atagctggtt ctccccgaaa
      901 gctatttagg tagcgcctcg tgaattcatc tccgggggta gagcactgtt tcggcaaggg
      961 ggtcatcccg acttaccaac ccgatgcaaa ctgcgaatac cggagaatgt tatcacggga
     1021 gacacacggc gggtgctaac gtccgtcgtg aagagggaaa caacccagac cgccagctaa
     1081 ggtcccaaag tcatggttaa gtgggaaacg atgtgggaag gcccagacag ccaggatgtt
     1141 ggcttagaag cagccatcat ttaaagaaag cgtaatagct cactggtcga gtcggcctgc
     1201 gcggaagatg taacggggct aaaccatgca ccgaagctgc ggcagcgacg ctgatgcgtt
     1261 gttgggtagg ggagcgttct gtaagcctgt gaaggtgtgc tgtgaggtat gctggaggta
     1321 tcagaagtgc gaatgctgac ataagtaacg ataaagcggg tgaaaagccc gctcgccgga
     1381 agaccaaggg ttcctgtcca acgttaatcg gggcagggtg agtcgacccc taaggcgagg
     1441 ccgaaaggcg tagtcgatgg gaaacaggtt aatattcctg tacttggtgt tactgcgaag
     1501 gggggacgga gaaggctatg ttggccgggc gacggttgtc ccggtttaag cgtgtaggct
     1561 ggttttccag gcaaatccgg aaaactaagg ctgaggcgtg atgacgaggc actacggtgc
     1621 tgaagcaaca aatgccctgc ttccaggaaa agcctctaag catcaggtaa catcaaatcg
     1681 taccccaaac cgacacaggt ggtcaggtag agaataccaa ggcgcttgag agaactcggg
     1741 tgaaggaact aggcaaaatg gtgccgtaac ttcgggagaa ggcacgctga tatgtaggtg
     1801 aagtccctcg cggatggagc tgaaatcagt cgaagatacc agctggctgc aactgtttat
     1861 taaaaacaca gcactgtgca aacacgaaag tggacgtata cggtgtgacg cctgcccggt
     1921 gccggaaggt taattgatgg ggtcagcgca agcgaagctc ttgatcgaag ccccggtaaa
     1981 cggcggccgt aactataacg gtcctaaggt agcgaaattc cttgtcgggt aagttccgac
     2041 ctgcacgaat ggcgtaatga tggccaggct gtctccaccc gagactcagt gaaattgaac
     2101 tcgctgtgaa gatgcagtgt acccgcggca agacggaaag accccgtgaa cctttactat
     2161 agcttgacac tgaacattga gccttgatgt gtaggatagg tgggaggctt tgaagtgtgg
     2221 acgccagtct gcatggagcc gaccttgaaa taccaccctt taatgtttga tgttctaacg
     2281 ttgacccgta atccgggttg cggacagtgt ctggtgggta gtttgactgg ggcggtctcc
     2341 tcctaaagag taacggagga gcacgaaggt tggctaatcc tggtcggaca tcaggaggtt
     2401 agtgcaatgg cataagccag cttgactgcg agcgtgacgg cgcgagcagg tgcgaaagca
     2461 ggtcatagtg atccggtggt tctgaatgga agggccatcg ctcaacggat aaaaggtact
     2521 ccggggataa caggctgata ccgcccaaga gttcatatcg acggcggtgt ttggcacctc
     2581 gatgtcggct catcacatcc tggggctgaa gtaggtccca agggtatggc tgttcgccat
     2641 ttaaagtggt acgcgagctg ggtttagaac gtcgtgagac agttcggtcc ctatctgccg
     2701 tgggcgctgg agaactgagg ggggctgctc ctagtacgag aggaccggag tggacgcatc
     2761 actggtgttc gggttgtcat gccaatggca ctgcccggta gctaaatgcg gaagagataa
     2821 gtgctgaaag catctaagca cgaaacttgc cccgagatga gttctccctg actccttgag
     2881 agtcctgaag gaacgttgaa gacgacgacg ttgataggcc gggtgtgtaa gcgcagcgat
     2941 gcgttgagct aaccggtact aatgaaccgt gaggcttaac cttacaacgc cgaaggtgtt
     3001 ttggcggatg agagaaagat tttcagcctg atacagatta aatcagaacg cagaagcggt
     3061 ctgataaaac agaatttgcc tggcggccgt agcgcggtgg tcccacctga cccc
//
LOCUS       DAZPEB010000288         3087 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZPEB010000288 DAZPEB010000000
VERSION     DAZPEB010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.294"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..191)
                     /locus_tag="ACHURJ_004056"
     CDS             complement(<1..191)
                     /locus_tag="ACHURJ_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HFR1282775.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQR"
     gene            384..886
                     /locus_tag="ACHURJ_004057"
                     /pseudo
     CDS             384..886
                     /locus_tag="ACHURJ_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(932..1348)
                     /gene="rraB"
                     /locus_tag="ACHURJ_004058"
     CDS             complement(932..1348)
                     /gene="rraB"
                     /locus_tag="ACHURJ_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFR1282776.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1510..2514
                     /gene="argF"
                     /locus_tag="ACHURJ_004059"
     CDS             1510..2514
                     /gene="argF"
                     /locus_tag="ACHURJ_004059"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFR1282777.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIAETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            complement(2586..>3087)
                     /locus_tag="ACHURJ_004060"
     CDS             complement(2586..>3087)
                     /locus_tag="ACHURJ_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR1282778.1"
                     /translation="SRKLQELIWDKLVAYVNDFNLSNQEKSRLILRLFDDVKLLFNEV
                     PVSILVNDIFLKDFFMKQPDFAKWYFYQLLKKYEGEQLYLNELGYVYGNEEKTNEIVN
                     NNPGYVVEIFEEKMGNELKIRTRMMEILRDGKINICEYINKEQLEKLNPPEDLRIAIK
                     KLGWNN"
BASE COUNT          864 a          703 c          659 g          861 t
ORIGIN      
        1 cgttgtccgt taacttccat attagcatag agataacgct tacatttcat taatgcccat
       61 ggtaaataga tacctagcgt tataatcgtt aacaacacat tcaccacaca aataagaaaa
      121 tatctctcac ctgtaccatg aaaaacaaac gaatgggacg gaacatccat ttcattaata
      181 acttgagcca taaaaattcc ctgaattaaa aaatccattg atataattta tatatacaga
      241 cagtaaaatg ttctgccacg ttacaatact cttatcagta ataggctggc aaaaacttta
      301 ttgctttata agatgcttgc gcacgtaaga agagagtggt attaaacaaa tgtataatta
      361 ctttattttt attgggttaa actatgaata acattgcgcc gcaatcacct gtaatgcgtc
      421 gcctgacgct acaggataat cctgctattg cccgcgtcat tcgtcaggta tccgccgaat
      481 acggtcttac cgctgataaa ggctacaccg tcgccgatcc gaatcttgac gaactgtatc
      541 aagtgtatag tcagccgggt catgcatatt gggtggttga gtacgagggt gaagtggtcg
      601 gcggcggtgg gattgcgcca ttagcaggta gtgaatcgga catttgcgaa ctgcaaaaga
      661 tgtattttct tccggctatt cgcggtaaag ggctggcgaa aaaactggcc ttaatggcga
      721 tggagcaggc acgagagatg ggcttcaaac gctgctatct ggaaacgacc gctttttaaa
      781 ggaagccatt gcgctttatg agcatttggg ctttgagcat atcgactatg cacttggctg
      841 cacgggccat gttgattgtg aagtgcggat gttgcgcgaa ctctaaaaaa atgcccgcta
      901 gtgtgtgcta tcgggcattt taatcaatta attagtggcg aaccccgtca tcgtcttcat
      961 cgacaaaatc ttcatcgtcg ccgtcttcgc cattaggatc ttcaaaataa gtgccccaac
     1021 cgtcgtactc aacgtcaaat ttctccgcca atgtcatcag ttgttcaacc tgggcatcga
     1081 tcagatcggc attcaacgcg cactcgctga ggatgtcgca gcaaatcacg atatcaccat
     1141 cttcaacttc cagctcttct ggatcggtca cttcgtaacc gagtttaaac gcttcaactg
     1201 ctgctttttc cagggtttcc agatcgtctg cggaaagatg gtgttcgatg gtgtacagcg
     1261 cgtccggatc gctgccatct tccagtaatt cttcaataat caaacgagtt tcttcacgct
     1321 gttcttccag ttgttccggg tttgccatgg ctcattcctc ttaatgtcct gccgatactc
     1381 ttattgtcac ataccactga cattgcctcc acctttgtga caaagattta tgcttcagac
     1441 ttgcaaatga ataatcatcc atataaattg aattttaatt cattgaggcg ttagccacag
     1501 gagggatcta tgtccgggtt ttatcataag catttcctga aattactcga tttcacgcca
     1561 gctgaactca acagcctgct gcagttagcc gcgaagctga aagccgataa gaaaagcggc
     1621 aaagaagaag ccaaactcac tggtaaaaac atcgcgctca tcttcgaaaa agactcgacc
     1681 cgtacccgat gctctttcga agttgccgca tatgaccagg gtgctcgcgt tacttatctc
     1741 ggcccaagcg gcagtcagat tggtcataaa gagtcgatta aagacactgc ccgcgtgctg
     1801 ggtcgcatgt atgacggtat tcagtatcgc ggctatggtc aggagattgc cgaaacactg
     1861 gcggaatacg ctggcgtgcc ggtatggaac ggcctgacca atgagtttca ccccacgcag
     1921 ttgctggcgg atctcctcac catgcaggag catttacccg gtaaagcatt caacgaaatg
     1981 acgctggtct atgcaggtga cgcacgtaac aacatgggca attcgatgct cgaagctgcg
     2041 gcgcttaccg gtctggattt gcgtctggtc gcgccacagg catgctggcc ggaagctgcg
     2101 ctggttacgg aatgccgcgc cctggcacag caaaatggcg ggaatattac gctgacggaa
     2161 gatgtagcca aaggagttga aggtgctgac tttatctata ccgatgtatg ggtgtcgatg
     2221 ggggaagcaa aagagaaatg ggcagaacgg attgcattac tgcgtgatta tcaggtgaac
     2281 agcaagatga tgcagttgac cggtaacccg gaggtcaaat tcctccactg cctgcccgcg
     2341 tttcatgacg accagacgac gcttggtaag aaaatggcgg aagagtttgg cctacatggc
     2401 ggaatggaag tgactgatga ggtcttcgaa tctgccgcca gcattgtttt tgatcaggcg
     2461 gaaaaccgca tgcataccat caaagcggtg atggtcgcga cgctcagtaa ataattgata
     2521 tcgcaataaa gatgccggga aatacaaaaa catcccggca tcacattatt ttatgcagta
     2581 atagttcaat tattccaccc caattttttt attgctatac gtaaatcttc cggaggattt
     2641 aatttttcta actgttcttt attaatatat tcacatatat ttattttccc atcccggagg
     2701 atttccatca ttcttgttct aatttttaat tcattaccca tcttttcttc aaaaatctca
     2761 acgacataac ccggattgtt atttacaatt tcatttgttt tttcttcatt accataaaca
     2821 tatcctaact cattaagata cagctgttcg ccctcatact tcttaagtaa ttgatagaag
     2881 taccacttcg caaaatcagg ttgcttcatg aaaaaatcct tcaaaaaaat atcatttact
     2941 aaaatgctga caggcacttc attaaatagc aacttaacat catcaaataa ccttagtatt
     3001 aagcgtgatt tttcttgatt gcttaagtta aaatcattga catatgcaac caacttatcc
     3061 caaatcaatt cctgtaattt acggcta
//
LOCUS       DAZPEB010000289         3011 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZPEB010000289 DAZPEB010000000
VERSION     DAZPEB010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.295"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            260..1318
                     /gene="citC"
                     /locus_tag="ACHURJ_004061"
     CDS             260..1318
                     /gene="citC"
                     /locus_tag="ACHURJ_004061"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFR1282779.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLKTPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1333..1629
                     /gene="citD"
                     /locus_tag="ACHURJ_004062"
     CDS             1333..1629
                     /gene="citD"
                     /locus_tag="ACHURJ_004062"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFR1282780.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGTLDCILRARLEALLARASGIPTLPWEDCQ"
     gene            1626..2534
                     /gene="citE"
                     /locus_tag="ACHURJ_004063"
     CDS             1626..2534
                     /gene="citE"
                     /locus_tag="ACHURJ_004063"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFR1282781.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2545..>3011
                     /locus_tag="ACHURJ_004064"
     CDS             2545..>3011
                     /locus_tag="ACHURJ_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFR1282782.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIHRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSG"
BASE COUNT          757 a          835 c          738 g          681 t
ORIGIN      
        1 aaattttatt cattaaatca attaaatcac ttaaacctgc aggtcatttc gtaattacat
       61 taattaaaaa acttaaacca ttaaataaat aaaaatttca cgccgaaata ctccttagga
      121 tgtatagcga aaggagaaaa agatatacct cgatcacccc cctttctccc aagtgaaaat
      181 aaaaggttat cagtttgcaa cattgaacaa cattcgttgc aaatcgataa caacatgcac
      241 cttcaggata ctatttatta tgttcggcaa tgatattttc acccgcgtaa aacgttcaga
      301 aaataaaaaa atggcggaaa tcgcccaatt cctgcatgaa aatgatttga gcgttgacac
      361 cacagtcgaa gtatttatta ccgtaacccg cgatgaaaag cttatcgcgt gcggtggaat
      421 tgccggaaat attattaaat gcgttgctat cagtgaatcc gttcgcggtg aaggactggc
      481 gctgacatta gccactgaac tgataaacct cgcctatgag cggcacagca cgcatctgtt
      541 tatttatacc aaaaccgaat acgaggcgct gttccgccag tgcggttttt ccacgctgac
      601 cagcgtaccc ggcgtgatgg tgctgatgga aaacagcgcc acgcgactga aacgctatgc
      661 cgaatcgctg aaaaaatttc gtcatccagg gaacaagatt ggctgcattg tgatgaacgc
      721 caatcccttt acgaatggtc accgttatct gattcaacaa gctgcggcac agtgcgactg
      781 gttgcatctg tttttagtca aagaagattc ttcacgcttc ccctatgaag accggctgga
      841 tctggtgtta aaaggcaccg ccgatattcc acgcctgact gtgcatcgcg gctccgaata
      901 catcatctcc cgcgctacgt tcccttgcta cttcattaaa gaacagagcg tcattaacca
      961 ttgttacacc gaaattgatc tgaagatttt ccgtcagtac ctcgctcccg cactgggtgt
     1021 aactcaccgc tttgtcggta ctgaaccctt ttgtcgcgtt accgcccagt acaaccagga
     1081 tatgcgctac tggctgaaaa cgccgactat ctccgcaccg cccatcgaac tggttgaaat
     1141 tgagcggctg cgttaccagg agatgccgat atccgcttcc cgggtacgtc aactgctggc
     1201 gaaaaacgat ctcacggcta tcgcgccgct ggtccctgca gtcacgctgc attatttgca
     1261 gaacctgctt gagcactccc gccaggacgc ggcagctcgt caaaagaccc ccgcatgaga
     1321 aacaggtgaa aaatgaaaat aaaccagccc gccgttgcag gcacccttga gtctggggat
     1381 gtgatgatac gcatcgcccc actcgatacg caggatatcg acctgcaaat caacagcagc
     1441 gttgagaaac agtttggcga tgcaattcgc accaccattc tggacgttct cgcccgctac
     1501 aacgtgcgcg gcgtacagct gaatgtcgat gacaaaggca cactggactg cattttacgt
     1561 gcacgactgg aagccctgct ggcacgcgcc agcggtatcc cgactctgcc atgggaggat
     1621 tgccaatgat ttccgcttcg ctgcaacaac gtaaaactcg cacccgccgc agcatgttgt
     1681 ttgtgcctgg tgccaatgcc gcgatggtca gcaactcctt catctacccg gctgatgccc
     1741 tgatgtttga cctcgaagac tccgtggcat tacgtgaaaa agacaccgcc cgccgcatgg
     1801 tttatcacgc gctgcaacat ccgctgtatc gcgatattga aaccattgtg cgtgtcaacg
     1861 cgctggattc cgaatggggt gttaacgacc tggaagccgt cgttcgcggt ggtgcggacg
     1921 ttgtgcgtct gccgaaaacc gataccgctc aggatgttct ggatattgaa aaagagatcc
     1981 tgcgtatcga aaaagcctgt ggtcgtgaac ccggcagcac cggcctgctg gcggcgattg
     2041 aatctccgct gggcattacc cgcgcagtgg aaatcgctca cgcttccgag cgtttgatcg
     2101 gtatcgccct cggtgcagaa gactatgtgc gcaacctgcg tacagaacgc tccccggaag
     2161 gaactgaact gctgttcgca cgctgttcca ttttgcaggc cgcgcgctct gcgggtattc
     2221 aggcgttcga taccgtctat tccgacgcta acaacgaagc cggattcctg caagaagccg
     2281 cccacatcaa acagctgggc tttgacggca aatcgctgat caacccgcgt cagattgatc
     2341 tgctgcacaa cctctacgca ccgacccaga aagaagtgga tcacgcccgc cgcgtcgtag
     2401 aagccgctga agccgccgct cgcgaaggcc tcggcgtggt ttccctgaac ggcaagatgg
     2461 tggacggtcc ggttatcgat cgcgcccgtc tggtgctctc ccgtgcagaa ctttccggca
     2521 tccgcgaaga ataaggcaat caaaatgacg cagaaaattg aacaatctca acgacaagaa
     2581 cgggtagcgg cctggaatcg tcgcgctgaa tgcgatcttg ccgctttcca gaactcacca
     2641 aagcaaacct accaggctga aaaagcgcgc gatcgcaaac tgtgtgccaa cctggaagaa
     2701 gcgattcatc gctctggttt acaggacggc atgacggttt ccttccatca cgctttccgt
     2761 ggcggtgacc tgaccgtcaa tatggtgatg gacgtcatcg cgaagatggg ctttaaaaac
     2821 ctgactctgg cctccagctc cctgagtgat tgccatgcgc cgctggtaga acacattcgc
     2881 cagggcgtgg ttacccgcat ttatacctct ggcctgcgcg gtccactggc ggaagaaatc
     2941 tcccgtggtc tgctggcgga accggtgcaa atccactctc acggcggacg tgtgcatctg
     3001 gtacagagcg g
//
LOCUS       DAZPEB010000290         2928 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZPEB010000290 DAZPEB010000000
VERSION     DAZPEB010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.296"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..756
                     /locus_tag="ACHURJ_004065"
     CDS             <1..756
                     /locus_tag="ACHURJ_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HFR1282783.1"
                     /translation="VTKSLCVIADKLRAFVYASCHGCNTMAEAITYRQKFNEREVMLL
                     WPDFIAYNPKNGKNETFPAPAYACGLRAYIDHEQGWHKSLSNVPVKNVLGMSRHVFWS
                     LQAEDSDANSLNNKEITTIIRRNGFRFWGNRTPETNAYIFEVYTRTAQVLADSIAEAQ
                     FETIDSPLTPANVKDVISAIRAKLDSLVTAGKLIGAECWYDVVDNSTTDLRQGRVRIR
                     YKYTPVPPLEDMELYQTFTDEYFEPAFAVLGGA"
     gene            756..1266
                     /locus_tag="ACHURJ_004066"
                     /pseudo
     CDS             756..1266
                     /locus_tag="ACHURJ_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310671.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
     gene            1388..1696
                     /locus_tag="ACHURJ_004067"
     CDS             1388..1696
                     /locus_tag="ACHURJ_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1282784.1"
                     /translation="MAVERGVKLTRPIERGGEKITYVEITGAIEQAGSLRGLSLSDVL
                     NLKADTMFTLLPRVTSPRLDEVMIKKMSSRDFIQLCAVAVNFMSEPDSGAKSVQETAA
                     "
     gene            1705..1806
                     /locus_tag="ACHURJ_004068"
                     /pseudo
     CDS             1705..1806
                     /locus_tag="ACHURJ_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333494.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
     gene            1847..>2928
                     /locus_tag="ACHURJ_004069"
     CDS             1847..>2928
                     /locus_tag="ACHURJ_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR1282785.1"
                     /translation="MKTLDIRVAFSAVDRLTRPAENARRLMGQFGDSIQRTQGAIKNL
                     ERQARSFERARDAVSKADAGIVKARRQLNALNQLQRTGTVLSEKQQKLMQQLSTRLER
                     LNESRTREIQKMRELGGELKRHGISLTGSDNTIQQAIRRTEQYNNQLERERQALARVT
                     RARERYSRAQETAGKLKTGGALAIGAAAAGGYAAGRFLQPAIGFGKEMSRVQALTRID
                     QNSPQFKALREQALKLGSETQFTAGDAASGQAFLAMAGFTPQAIQAALPGVLSMATAG
                     GMDLGETADIGSNILTQFGLSADQMDRVGDTLTAAFTRTNTDLRALGETMKYAGPVAG
                     KLGISLEQAAAMAGVLANMGIRGSDAG"
BASE COUNT          703 a          733 c          878 g          614 t
ORIGIN      
        1 gtaacaaaat ccctgtgcgt gattgcagat aaactgcgcg cgtttgtgta tgcctcatgt
       61 catggctgta acacgatggc cgaggcaatt acctaccgcc agaaattcaa cgaacgtgag
      121 gtgatgctct tatggccgga cttcatcgcc tacaacccga aaaatggcaa aaacgaaacg
      181 ttccccgcgc ctgcctatgc gtgcggcctt cgtgcgtaca ttgaccatga acagggatgg
      241 cacaaatcgc tgtccaacgt tccggttaaa aatgtgctgg ggatgtcgag gcatgtgttc
      301 tggtcgttgc aggccgaaga cagcgatgcc aacagcctca acaacaaaga aatcacgacc
      361 attattcgtc gcaacgggtt ccgcttctgg ggtaaccgca caccggaaac gaacgcctac
      421 atctttgagg tgtatacccg aaccgcacag gtgctggctg attcaattgc ggaagcgcag
      481 tttgaaacca tcgacagtcc actgacgcct gcgaatgtga aggatgttat cagtgccatc
      541 agggcaaaac tggattcact ggtgactgcc gggaaactga ttggcgcgga gtgctggtat
      601 gacgtggtgg ataacagcac cacggattta cgtcagggac gtgtgcgtat tcgctacaaa
      661 tatacgcccg ttccgccact ggaagacatg gagctttacc agacgtttac tgatgaatac
      721 tttgaacccg catttgcggt gctgggaggt gcctgatggc tgtgccaaaa catcttcgct
      781 tttttacgct gtttgtggat ggtgaaaacg aagtgggtaa tgtgacgtcc gtcactctgc
      841 ctaagctgac gcgcaaaacc gacagctacc ggggtggtgg catgatgggt gcggtaagta
      901 ttgatctcgg tctggacgac tccgcgcttg atgcgagctt tgtcatgggg gcgcagttcg
      961 tgagctgttc cttaagtatg gcggcacgat tgacggcacg ctgctgcgtt ttgcgggtga
     1021 atactacacc gatgcagaaa gcgacctgta tgaagtcgaa atgcgcggac gtgtgacgga
     1081 aattgatatg ggggaagcca aacagggcga agccacatca cacacttacg ccattaaaac
     1141 acctactaca agctgagtgt taacgatcgc ccgttgtggg agattgacct gctgaacttc
     1201 atttaccgga aggacggcaa ggacattgtg cccgatcgca tccgttccgc gcttgggctt
     1261 ggctgataag taatatgctg gcggcgcagt gcgtcgcctc tgactgaaaa ggagacaaca
     1321 gatgaaagac atcgatactg aaacccggaa taacaccgtg gcggataatg tgacggcagt
     1381 tgaggatatg gctgtcgaac gtggcgtaaa actgacccga ccaattgagc gtggtggcga
     1441 aaaaatcacg tatgtggaga tcaccggggc tattgagcag gctggatccc tgcgtggtct
     1501 gtcgctgtct gatgtgctga atctgaaagc ggataccatg ttcacgctgt tgcctcgcgt
     1561 gacctcgcca cgactggatg aagtgatgat taaaaaaatg tcgtcacgcg attttattca
     1621 gttgtgcgct gtggctgtaa attttatgag cgagccagac tctggcgcga agagcgtgca
     1681 ggagacggca gcgtaatcac cctggtgtgc tttgagcaca tcgaagatct ggtggcggat
     1741 attgccgcca tttttaactg gtcgcccgcc gaaatcttca tgatgacgcc cggcgaagtg
     1801 gttagctgac gtgagcgggc ggcacttcgt agcgggaatg cagacaatga agactcttga
     1861 tatccgggtc gctttcagcg ccgttgacag gctgacccgg cctgccgaaa acgcccgccg
     1921 cctgatgggg cagtttggtg actccatcca gcgaacgcag ggggcgatca aaaatctcga
     1981 gcgtcaggcg cgatcatttg agcgcgcccg tgacgctgtc agtaaagcgg atgctggcat
     2041 cgtgaaagca cgacgccagc ttaacgccct taatcagtta caacgcacgg gtacagtgct
     2101 cagcgaaaaa caacaaaagc tgatgcagca gttaagcacc cggcttgaac gcctgaatga
     2161 atcgcgcaca cgggaaattc agaaaatgcg ggagcttggc ggagagctga aacgccacgg
     2221 catttccctg acaggcagcg ataacaccat ccagcaggcc atcagacgca ccgaacagta
     2281 caacaaccag cttgaacgcg aacggcaggc gcttgcgcgt gtaacacggg cgcgtgagcg
     2341 gtattcgcgc gcgcaggaaa cagcgggaaa actgaaaaca ggtggtgcgc tggcaattgg
     2401 tgcggcagcg gcgggcggct atgctgccgg gcgttttttg cagcctgcga tcgggttcgg
     2461 caaagagatg tcccgcgttc aggcactgac gcgaatcgac cagaacagcc cgcagtttaa
     2521 ggcgctgcgt gagcaggcgt taaaacttgg ctctgaaacg cagttcaccg caggcgatgc
     2581 cgccagtgga caggcatttc ttgcaatggc tggctttaca ccacaggcca ttcaggctgc
     2641 gctccctggc gtactgagca tggcaacggc tggcggtatg gacctcggtg agacggcgga
     2701 tattggctca aatattctga cgcagttcgg tctttctgct gaccagatgg accgggtcgg
     2761 cgacacactc accgcagcgt ttacccgtac caacactgac cttcgcgcac tgggtgaaac
     2821 catgaaatat gcaggtccgg tggcgggtaa gctgggaata tctctggagc aggccgcagc
     2881 gatggcgggc gtgctggcga atatgggtat cagggggagt gatgctgg
//
LOCUS       DAZPEB010000291         2920 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZPEB010000291 DAZPEB010000000
VERSION     DAZPEB010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.297"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..1323
                     /locus_tag="ACHURJ_004070"
     CDS             <1..1323
                     /locus_tag="ACHURJ_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFR1282786.1"
                     /translation="GLVVVFSWVFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHT
                     MGPGLAYLAAWTYWVVHIPYLAQKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVL
                     FIFFMWVASRGMKSLKIVGSVAGIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETF
                     IPHIDFTYITTISMLVFAVGGAEKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGS
                     LAMGMMFDSRNIPDDLMTNGQYYAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSI
                     DAPLKVLLGDADSKYIPASLCRTNASGTPVNGYFLTLVQVAILIMLPTLGIGDMNNLY
                     KWLLNLNSVVMPLRYLWVFVAFIAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAF
                     ACLTGIFPKMEAFTAEWTFQLALNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(1324..2232)
                     /locus_tag="ACHURJ_004071"
     CDS             complement(1324..2232)
                     /locus_tag="ACHURJ_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1282787.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINDARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNI
                     ILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLGDWSTERPGFQIYYPNRQY
                     MSCGLRAFLDYVKTGQISQSQRHRPQ"
     gene            2332..>2920
                     /locus_tag="ACHURJ_004072"
     CDS             2332..>2920
                     /locus_tag="ACHURJ_004072"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HFR1282788.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTPEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPLWWYSFPAMLKGYI
                     DRVWNNGLAYGDGHKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGEELHASYYQSLLSQVRDMVDAL"
BASE COUNT          738 a          621 c          729 g          832 t
ORIGIN      
        1 gggctggtgg ttgttttttc atgggtgttt atctttgcgc tctatttcac accttatgcg
       61 ctaattgttg gtcagttagg ctcgaccttc aaagatggga agggcggggt cagtacctgg
      121 attaaacaca cgatgggacc cggactggct tatctcgccg cgtggaccta ctgggtggtg
      181 catattccct atctggcaca aaaaccccag gcaattctga ttgcgctcgg ttgggcgatg
      241 aaaggcgacg gttcgctaat caaagaatat tcagtcgtag cgttacaggg gttaacgctg
      301 gtgctgttta tcttctttat gtgggttgct tcacgcggta tgaaatcgct gaaaatcgtc
      361 ggttctgtgg cagggattgc gatgtttgtt atgtcgctcc tgtatgtggc gatggcggta
      421 accgcgcctg cgattactga agtgcatatt gcgaccacaa acattacctg ggaaacgttc
      481 attcctcata tcgactttac ctacattacc actatttcaa tgctggtttt cgcggttggc
      541 ggagcagaga agatttctcc ttacgttaat caaacgcgca acccaggaaa agaatttcca
      601 aaagggatgt tatgcctggc ggtgatggtt gcggtttgtg ccattctggg ctcactggcg
      661 atggggatga tgtttgactc gcgtaatatc ccggatgact taatgactaa cggtcaatat
      721 tacgcctttc agaagctggg cgagtattac aacatgggta atactttaat ggtgatttac
      781 gccattgcga ataccctggg acaagtagct gcgctggtat tctcgattga tgccccgctt
      841 aaagtgctat taggtgatgc tgacagcaaa tatattccag ccagtttatg tcgtaccaac
      901 gcttctggta cgcccgttaa tggctatttt ctgaccctgg tgcaggtggc aattctgatt
      961 atgctgccga ctctcggcat cggtgatatg aacaatctct ataaatggct gttgaatctt
     1021 aactcggtag tgatgccgct gcgttattta tgggtatttg ttgcatttat tgcagtcgtt
     1081 cgcctggcgc agaaatataa accagaatat gtctttattc gtaataagcc tctggcaatg
     1141 accgtcggga tttggtgttt tgcctttacc gcctttgcct gtttgacggg gatcttcccg
     1201 aaaatggaag ccttcactgc agagtggacc ttccagttgg cgctgaatgt tgcaacgccg
     1261 tttgtgctgg taggattagg actgattttc ccgctgctgg cgcgtaaagc gaatagtaaa
     1321 taattattgt ggtcggtgac gctgactctg gctgatctga cccgttttta cataatcgag
     1381 aaatgcgcgc aagccgcagg acatatactg gcggtttgga taatagatct ggaagcctgg
     1441 acgctctgtg ctccagtctc ccagtacaca ttctaaacga ccagtatcaa ggtactcctt
     1501 gatctgttca tagagcagat acccgatacc agcacccatc aaaacggcct ccagttcagc
     1561 atcaacatca tccagaatga tatttcctgc gacagcaatt tccagctcct ttgcaaattg
     1621 ccagtggaaa ggtttgccgc tgggataacg aaaaacaaca cattgatgat taagcagatc
     1681 atgaggatgg cggggtttgc catatcttgc aaagtactct ggcgttgctg ctacacataa
     1741 tttcactggc gggccgatcg ccacgctgat catatctttt tcgacgatgc cactaagtcg
     1801 aacgccagcg tcaaagccct gttggacgat atcgacaaga gagtcgtcag ttgtgagctc
     1861 gactttaata tcaggatatt cacgtgtgaa accaaccagt aaaggcatta aaaatatacg
     1921 tgcagctacc cgggcgtcat tgattttgag cgttcctgtc ggtgtcagac gaaaatcatt
     1981 catctcatct aacatgatct gaatttcgtc gaaagctggg cgcaatcgtt catagagatt
     2041 agaacctgct tccgttaacg atacgcttcg ggttgtccga ttgaataagc gaattttaag
     2101 acgttgttcc agtgttttaa tactatggct aatggcggac gaggataagc ctaactcatc
     2161 gcccgctgca cgaaagcttt gattacgggc cacggcaaag aaagtggcaa agtcagacat
     2221 attcatccgc attagtgaaa atccttcatt aactcatcct gaatcatcac gtttacagga
     2281 ttatgccgcc tgcgtaaagt gcgctccaga acttaacgtg gaggtaaaat tatgcagtct
     2341 gaacgtattt atttggtatg ggcccatcct cgtcatgatt cattgaccgc acatattgct
     2401 gatgcgatcc atcagcgggc aatggagcgg aaaatacagg tgacggaact cgatttatat
     2461 cggcgtaatt tcaacccagt gatgacgccg gaagatgaac cagactggaa gaatatggat
     2521 aaacgttatt ctccagaggt tcatcagctt tattcagagc tgcttgaaca tgacacgtta
     2581 gtggtggttt ttcctctctg gtggtacagc ttcccggcaa tgctaaaagg atatattgac
     2641 agagtatgga ataatgggct ggcttatgga gatgggcaca aattaccatt caataaagtt
     2701 cgttgggtgg cgctggttgg aggagacaaa gaatcatttg tccagatggg ctgggaaaaa
     2761 aatataagcg attatttgaa aaatatgtgc agttatcttg gtattgaaga tgccgatgtc
     2821 actttcttgt gtaatacagt ggtattcgat ggggaagaac ttcatgcgag ctattatcag
     2881 tcgttattat ctcaggtacg ggatatggta gatgcactat
//
LOCUS       DAZPEB010000292         2920 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZPEB010000292 DAZPEB010000000
VERSION     DAZPEB010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.298"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..756
                     /locus_tag="ACHURJ_004073"
     CDS             <1..756
                     /locus_tag="ACHURJ_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HFR1282789.1"
                     /translation="VTKSLCVIAGKLRAFVYASCHGCNTMAEAITYRQKFNEREVMLL
                     WPDFIAYNPKSGKNETFPAPAYACGLRAYIDHEQGWHKSLSNVPVKNVLGMSRHVFWS
                     LQAEDSDANSLNNKEITTIIRRNGFRFWGNRTPETNAYIFEVYTRTAQVLADSIAEAQ
                     FETIDSPLTPANVKDVISAIRAKLDSLVTAGKLIGAECWYDVVDNSTTDLRQGRVRIR
                     YKYTPVPPLEDMELYQTFTDEYFEPAFAVLGGA"
     gene            756..1268
                     /locus_tag="ACHURJ_004074"
     CDS             756..1268
                     /locus_tag="ACHURJ_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFR1282790.1"
                     /translation="MAVPKHLRFFTLFVDGENEVGKVTSVTLPKLTRKTDSYRGGGMM
                     GAVSIDLGLDDSALDASFVMGGAVRELFLKYGGTIDGTLLRFAGEYYTDAESDLYEVE
                     MRGRVTEIDMGEAKQGEATSHTYAIKNTYYKLSVNDRPLWEIDLLNFIYRKDGKDIVP
                     DRIRSALGLG"
     gene            1323..1688
                     /locus_tag="ACHURJ_004075"
     CDS             1323..1688
                     /locus_tag="ACHURJ_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1282791.1"
                     /translation="MKETKNIDTENTVVTDTVKETSERGVKLTQPIERSGEKITYVEI
                     TGAIEQAGSLRDLSLSDVLSLKAESMFTLLSRVTSPRLDEVTIKKMASRDFIQLCVVA
                     VNFLSGADSGGKNEQATEA"
     gene            1697..1852
                     /locus_tag="ACHURJ_004076"
     CDS             1697..1852
                     /locus_tag="ACHURJ_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFR1282792.1"
                     /translation="MCFEHIEDFVADIAVIFNWSPAEIFMMTPGEVVSWRERAALRSG
                     NADNEDS"
     gene            1839..>2920
                     /locus_tag="ACHURJ_004077"
     CDS             1839..>2920
                     /locus_tag="ACHURJ_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR1282793.1"
                     /translation="MKTLDIRVAFSAVDRLTRPAENARRLMGQFGDSIQRTQGAIKNL
                     ERQARSFERARDAVSKADAGIVKARRQLNALNQLQRTGTVLSEKQQKLMQQLSTRFER
                     LNESRTREIQKMRELGGELKRHGISLTGSDNTIQQAIRRTEQYNNQLERERQALARVT
                     RARERYSRAQETAGKLKTGGALAIGAAAAGGYAAGRFLQPAIGFGKEMSRVQALTRID
                     KNSPQFKALREQALKLGSETQFTAGDAASGQAFLAMAGFTPQAIQAALPGVLSMATAG
                     GMDLGETADIGSNILTQFGLSADQMDRVGDTLTAAFTRTNTDLRALGETMKYAGPVAG
                     KLGISLEQAAAMAGVLANMGIRGSDAG"
BASE COUNT          711 a          725 c          877 g          607 t
ORIGIN      
        1 gtaacaaaat ccctgtgcgt gattgcaggt aaactgcgcg cgtttgtgta tgcctcatgt
       61 cacggctgta acacgatggc tgaggcgatt acctaccgcc agaaattcaa cgaacgtgag
      121 gtgatgctct tatggccgga cttcatcgcc tacaacccga aaagtggcaa aaacgaaacg
      181 ttccccgcgc ctgcctatgc gtgcggcctt cgtgcgtaca ttgaccatga gcagggatgg
      241 cacaaatcac tgtccaacgt tccggttaaa aatgtgctgg ggatgtcgag gcatgtgttc
      301 tggtcgttgc aggccgaaga cagcgatgcc aacagcctca acaacaaaga aatcacgacc
      361 attattcgtc gcaacgggtt ccgcttctgg ggcaaccgca caccggaaac gaacgcctac
      421 atctttgagg tgtatacccg aaccgcacag gtgctggctg attcaattgc ggaagcgcag
      481 tttgaaacca tcgacagtcc actgacgcct gcgaacgtga aggatgttat cagtgccatc
      541 agggcaaaac tggattcact ggttactgcc gggaaactga ttggcgcgga gtgctggtat
      601 gacgtggtgg ataacagcac cacggattta cgtcagggac gtgtgcgtat tcgctacaaa
      661 tatacgcccg ttccgccact ggaagacatg gagctttacc agacgtttac tgatgaatac
      721 tttgaacccg catttgcggt gctgggaggt gcctgatggc tgtgccaaaa catcttcgct
      781 tttttacgct gtttgtggat ggtgaaaacg aagtgggtaa ggtgacgtcc gtcactctgc
      841 ctaagctgac gcgcaaaacc gacagctacc ggggtggtgg catgatgggg gcggtaagta
      901 ttgatctcgg cctggacgac tccgcgcttg atgcgagctt tgtcatgggg ggcgcagttc
      961 gtgagctgtt ccttaagtat ggcggcacga ttgacggcac actgctgcgt tttgcaggtg
     1021 aatactacac cgatgcagaa agcgacctgt atgaagtcga aatgcgcgga cgtgtgacgg
     1081 aaattgatat gggggaagcc aaacagggcg aagccacatc acacacttat gccattaaaa
     1141 acacctacta caagctgagt gttaacgatc gcccgttgtg ggagattgac ctgctgaact
     1201 tcatttaccg gaaggacggc aaggacattg tgcccgatcg catccgttcc gcgcttgggc
     1261 ttggctgata agtaatatgc aggcggcgca gtgcgtcgcc tctgactgaa aggagtttcc
     1321 tgatgaaaga gacgaaaaac atcgataccg aaaacacggt agttactgac actgtgaaag
     1381 aaaccagtga gcgtggcgta aaacttaccc aaccaattga gcgaagcggc gaaaaaatca
     1441 cgtatgtgga gatcaccggg gctattgagc aggctggatc tctgcgagat ttgtcgctgt
     1501 ctgatgtgct gagtctgaaa gcggaatcca tgtttacgct gctgtcacgc gtgacatcac
     1561 cgcgactgga tgaagtgacg atcaaaaaaa tggcatcccg tgactttatt cagttatgtg
     1621 tggttgccgt aaattttttg agcggtgcgg actctggcgg gaagaacgaa caggcgacgg
     1681 aagcctgatc acggttgtgt gctttgagca catagaagac tttgtggcag atattgccgt
     1741 tatttttaac tggtcgcccg ccgaaatctt catgatgacg cccggcgaag tggttagctg
     1801 gcgtgagcgg gcggcacttc gcagcgggaa tgcagacaat gaagactctt gatatccggg
     1861 tcgctttcag cgccgttgac aggctgaccc gacctgccga aaacgcacgc cgcctgatgg
     1921 ggcagtttgg tgactccatc cagcgaacgc agggggcgat caaaaatctc gagcgtcagg
     1981 cgcgttcatt tgagcgcgcc cgcgacgctg tcagtaaagc ggatgcgggt atcgtgaaag
     2041 cacgacgcca gcttaacgcc cttaatcagt tacaacgcac gggtacagtg ctcagcgaaa
     2101 aacaacaaaa gctgatgcag cagttaagca cccggtttga acgcctgaat gaatcgcgca
     2161 cacgggaaat tcagaaaatg cgggagcttg gcggagagct gaaacgccac ggcatttccc
     2221 tgacaggcag cgataacacc atccagcagg ccatcagacg caccgaacaa tacaacaacc
     2281 agcttgaacg cgaacggcag gcgcttgcgc gtgtaacgcg ggcgcgtgag cggtattcgc
     2341 gcgcgcagga aacagcggga aaactgaaaa caggtggtgc gctggcaatt ggtgcggcag
     2401 cggcgggcgg ctatgctgcc gggcgttttt tgcagcctgc gatcgggttc gggaaagaga
     2461 tgtcccgcgt tcaggcactg acgcgaatcg acaaaaacag cccgcagttt aaggcgctgc
     2521 gtgagcaggc gttaaaactt ggctctgaaa cgcagttcac cgcaggcgat gccgccagtg
     2581 ggcaggcatt tcttgcaatg gctggcttca caccgcaggc cattcaggct gcgcttccgg
     2641 gcgtgctgag catggcaacg gctggcggta tggaccttgg cgagacggcg gatattggct
     2701 caaatatcct gacgcagttc ggcctctccg ctgaccagat ggaccgggtc ggtgacacgc
     2761 ttacagcagc gtttacccgt actaacactg accttcgcgc gctgggcgaa accatgaaat
     2821 atgcaggtcc ggtggcgggt aagctgggaa tatcgctgga gcaggccgca gcgatggcgg
     2881 gcgtgctggc gaatatgggt atcagaggga gtgatgccgg
//
LOCUS       DAZPEB010000293         2865 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZPEB010000293 DAZPEB010000000
VERSION     DAZPEB010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.299"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..1072)
                     /locus_tag="ACHURJ_004078"
     CDS             complement(<1..1072)
                     /locus_tag="ACHURJ_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFR1282794.1"
                     /translation="MAFKHYDVVRAASPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGAVISATSDPEYYFVVVLAGQSNGMSYGEGLPLPGTYDRPDPRIKQLARRST
                     VTPGGAACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGTVGQGLHIAKKLLPFI
                     PANAGILLVPCCRGGSAFTTGADGTYSDASGASENSTRWGVDKPLYKDLIGRTKAALE
                     KNPKNVLFAVVWMQGEFDFGGTPANHAAQFGALVDKFRADLADMAGQCVGGSAGGVPW
                     ICGDTTYFWKQKNESTYQTVYGSYKNKTEKNIHFVPFMTDENGVNVPTNKPEEDPDIR
                     GIGYYGSKWRDSSATWTSQDRAS"
     gene            1341..1457
                     /locus_tag="ACHURJ_004079"
                     /pseudo
     CDS             1341..1457
                     /locus_tag="ACHURJ_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1562..1638)
                     /locus_tag="ACHURJ_004080"
     tRNA            complement(1562..1638)
                     /locus_tag="ACHURJ_004080"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1602..1604),aa:Arg,seq:tct)
     gene            complement(1652..1728)
                     /locus_tag="ACHURJ_004081"
     tRNA            complement(1652..1728)
                     /locus_tag="ACHURJ_004081"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1692..1694),aa:Arg,seq:tcg)
     gene            complement(1736..1811)
                     /locus_tag="ACHURJ_004082"
     tRNA            complement(1736..1811)
                     /locus_tag="ACHURJ_004082"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1776..1778),aa:Ile,seq:cat)
     gene            complement(1852..2865)
                     /locus_tag="ACHURJ_004083"
                     /pseudo
     CDS             complement(1852..2865)
                     /locus_tag="ACHURJ_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
BASE COUNT          644 a          851 c          693 g          677 t
ORIGIN      
        1 ggctcgccct gtcctgtgac gtccaggtgg ctgagctgtc acgccatttc gaaccgtaat
       61 aaccgatacc ccgaatgtcc gggtcttctt ccggtttgtt cgtcggcaca ttcaccccgt
      121 tctcatcggt catgaacggt acgaaatgga tattcttttc cgttttattt ttatagctgc
      181 cgtacaccgt ctggtacgtg gattcgttct tctgcttcca gaaatacgtc gtgtccccgc
      241 atatccaggg aacaccgcca gcagagccac cgacgcactg gcctgccata tccgccaggt
      301 ctgcacggaa tttatcaacc agcgcaccaa actgtgcggc atgatttgcc ggcgtaccgc
      361 caaaatcaaa ttccccctgc atccacacca cggcaaacag cacatttttc gggttcttct
      421 ccagtgccgc ttttgttcga ccgataaggt ccttatacag cggcttgtcc acaccccagc
      481 gggttgaatt ctccgaggca ccactcgcgt cactgtatgt gccatcagct ccggtggtga
      541 acgctgaacc accacgacag cacggaacca gcagaatgcc cgcattcgcc ggtataaacg
      601 gcagcagttt tttggcgata tgcagcccct gccccacggt tccgtactgc ccctttgaca
      661 ggtccgcttt cggatggtta agacggctca tgtcctgcac atcatgcaga caatggtccg
      721 ccggaatgat gtcgttatat ttgcatgctg caccgcccgg tgtcaccgta ctgcgacgcg
      781 ccagttgctt aatacgcggg tccggacggt catatgtccc cggcagcgga aggccttcac
      841 catacgacat gccgtttgac tgccctgcca gaaccacaac aaagtaatac tccgggtcgc
      901 tggtggcgct gattactgca ccttctgctg caatcgcctg catcagagta taaggggtta
      961 tggccaccgg actaccaaac ggctgccagc cctctttcag tttatgtgtc agcttttccg
     1021 caagatctga cggcgacgcc gccctgacaa catcatagtg tttaaaagcc atgaatcctc
     1081 ccggccggga taatattgtg agtaaaatga ggagcgggct gaagtccgga agttacagga
     1141 caatggcaga agagagacga cagcccgcaa ttcgaaaaag accgcgcagt tgcgcagagt
     1201 gactactatg gggtattatt cgccagctga aatattactt cacgttttat tgtttattcc
     1261 ttgccgcccg cgtctcccag cgcgggcttt ttttgcccac aagaaagccc ctccggagag
     1321 gggctggaga gtggcgctat gtgccattgc atggtgccgg gtgcctcccg gtgaattcag
     1381 taccagcacc tgaatccgcg attatcccat atacctactc gctgattgcc cctccgcaca
     1441 gggggattca ccatgccagt ttcttttaac aaactccccg caaaccagac aacagtcaac
     1501 cgtctgaatt gtgagacatt taaaaaaaaa gcccgcaaaa gcgagccagg gaaaataagt
     1561 gtggcgcgtt gtactggatt cgaaccagtg accgattgct tagaaggcaa ttgctctgtc
     1621 cggctgagct aacaacgcat gatgcagata atggaccgcc atcggggact tgaaccccgc
     1681 gcagccagct tcgaaggctg gcgctctttc ctgatgagct aatggcggta tgtgatggtg
     1741 gcccttgctg gatttgaacc agcgacctgg cgattatgag tcgctcgctc tcaccactga
     1801 gctaaagggc cgggagccgc ataataacga cgcgtaatta attcttcaat atcatccgtt
     1861 ctggctgact aaatcctgta cttcccgaac cgtctgctca aaacgttcag tctccagctc
     1921 aacgccaatt gcacgacgcc cgagcgccag tgccgctttc actgtcgaac ccgaccccat
     1981 gaaaaaatct gcaatcaggt cacccggacg actgctagcg ctgattatct gctgcagcat
     2041 ttctgccggt ttttcgcacg gatgtttccc gggatagaac tgcaccggtt tatgcgtcca
     2101 cacatcggta tacggcacct gcaccgtcac gccaaaatac cgccgcagat gtttatattc
     2161 actcagcagc tccgcatact gccggttcag tgaggtatac gtatccacca gctggtggtg
     2221 gagcttttcc agttcaccac gccgatgctt ctcttctgcc acccgggcaa acagcgcctg
     2281 taatttcaga taatcgcttt cattcggcaa ctgccactga ctggcactga accagtgcga
     2341 caccatgttt ttctttcctg tggcatctgc aatctgtttt gccgttatcc ccagggcagc
     2401 tcgcgcatca cgaaagtaag aaatcagcgg ggccatcaca tgctgtttca gtgcactgcc
     2461 cttcgcctca tagccatcat ctttcgggcg gtacggcccc tgataatgtt ccgcgaacag
     2521 aatgcgctct gtggcgggga aatacgcccg caggctttcc ttgttgcatc cgttccagcg
     2581 tccggacggc ttcgctcaga taatatggtt cagcacactg aagcgttcac gcatcatgat
     2641 ttcgatatca gatgccaggc gatgaccaca gaacaggtaa agacttccgg caggtttcag
     2701 cacccgccag aactgcgcca gacactgctc cagccacttc aggtaatcat cgtcgccctt
     2761 ccactggtta tcccagccct caggcttcac tttaaagtac ggcgggtccg tgactatcag
     2821 gtcaacagaa ttttcgggta acgaccagat aaattccagg cagtc
//
LOCUS       DAZPEB010000294         2855 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZPEB010000294 DAZPEB010000000
VERSION     DAZPEB010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.300"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            47..346
                     /locus_tag="ACHURJ_004084"
     CDS             47..346
                     /locus_tag="ACHURJ_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFR1282795.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            356..880
                     /gene="ygaP"
                     /locus_tag="ACHURJ_004085"
     CDS             356..880
                     /gene="ygaP"
                     /locus_tag="ACHURJ_004085"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFR1282796.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDRIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(927..1331)
                     /gene="stpA"
                     /locus_tag="ACHURJ_004086"
     CDS             complement(927..1331)
                     /gene="stpA"
                     /locus_tag="ACHURJ_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFR1282797.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            1998..2447
                     /gene="alaE"
                     /locus_tag="ACHURJ_004087"
     CDS             1998..2447
                     /gene="alaE"
                     /locus_tag="ACHURJ_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFR1282798.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(2484..2828)
                     /locus_tag="ACHURJ_004088"
     CDS             complement(2484..2828)
                     /locus_tag="ACHURJ_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFR1282799.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
BASE COUNT          764 a          653 c          630 g          808 t
ORIGIN      
        1 atttttagtt tagagtttac ttaatttaga aaatacttaa ataaatatga ctgaactcgc
       61 gcaattacag gccagtgccg aacaggcagc ggccttattg aaagcaatga gccatcctaa
      121 acgattgctg attctgtgca tgcttagcgg ttcccccggc accagcgcgg gagagctgac
      181 gcgcattacc ggactgagtg cctctgcgac atcacagcat ctcgctcgta tgcgggacga
      241 agggcttatc gacagccaac gggatgccca acgcattcta tattccatta aaaatgaggc
      301 ggtaaatgcc attatcgcca ccctgaaaaa tgtctattgt ccgtaaggag tcactatggc
      361 tttgacaacc atttcgccgc atgatgcaca agaattaatc gcacgcggcg caaagttaat
      421 cgatattcgt gacgctgatg aatatcttcg tgaacatatt cctgaagcag atctggctcc
      481 attatccgtg ctggaacagt caggtcttcc acctaaatta cgtcgcgagc aaattatatt
      541 ccactgccag gcaggtaaac gcaccagtaa taacgccgat aaattagcag cgattgccgc
      601 ccccgcagaa atatttttac tcgaagatag gattgatggc tggaaaagag ctggattgcc
      661 agtagcggta aataaatctc aacccttgcc gttaatgcgc caggtgcaga tcgctgcggg
      721 tggtttaata ttaatcggcg ttgtactggg ttataccgta aatagcggtt tcttcttatt
      781 aagtggcttt gttggtgccg ggttactgtt tgcaggaatc agcggttttt gtggaatggc
      841 aaggttgtta gataagatgc cgtggaacca acgagcttga gaagcgacgc cggacgcgcc
      901 ctagcagcga catccggcct cagtaattag atcaggaaat cgtcgagaga tttaccttct
      961 gccagcgcct gagcaattgg cttcggtgta cggccctgac cggtccaggt cttagtttca
     1021 ccgttaacat cagtgaattt atatttcgcc ggacgcggct ggcgtttttt accagcacgt
     1081 ggtgcagcag cagagctatt acccaataac tcttccgggt taattccgtc agctttcatc
     1141 agctccagcc aggtgctaat tttttcctga cgctcagcca gttcacgctg ctgctgttct
     1201 tcttcttcac gtctttcttt agtgacaacc ctgaattttt cgagcatttc ttcaagaacg
     1261 tcaatggaga attcgcgagc catcgcacgg agggttcgaa tgttatttaa actttgtaac
     1321 attacggaca taataaagaa aaccttttaa cgccaaaaca aaaaagtatt tcgtcgcgta
     1381 gtgtaagcga tcacttatta tatttccaca aaatttaatc tatttacagt cctgcgcgag
     1441 attatttcag ctgttatttt ctgaaactct cagaataatt tcaagattat atattagcca
     1501 ttttgatgag taatttaggt tattggtgaa cagagtggcg ctctctgctc gctaactccg
     1561 caagcctgat aaaaccgaag tgaataaaaa tgcaatataa cagaataaaa gaaaacaccc
     1621 ctcactggtg ctttctgctg ttaatgtaca ttttctggca tcaaaaatgc tgtaaatgac
     1681 ccggtcacga caaacaccga attctctacg cttatagcgt tacctcaccc ccaaactttc
     1741 attagtgaaa ttaaaggcaa ggtcatatcc aattgattta attgaaaata aatcatatcg
     1801 gcatgtaaaa atccacaatg tacaaaaaac gagctgttac ggaatatttt atctacaaaa
     1861 actgactaaa taaaagtttt tcactaattg attagtcata gccagcgata tacgctatgc
     1921 gaaaatgcag atggcaatga gatccactgc tttcatctcc attaacatcc cattacgctt
     1981 tattaaggag cattagcatg ttctcaccgc agtcacgctt gcgtcatgca gttgcagata
     2041 cgttcgcgat ggttgtttac tgttctgtcg tgaacatgtg tattgaagtt ttcctctccg
     2101 gaatgagctt cgaacagtct ttttattcca gattggtagc gattccggtg aacatcttaa
     2161 ttgcatggcc atacggtatg taccgcgatc tgtttatgcg cgcggcacgc aaagttagcc
     2221 cgtcgggctg gataaaaaat ctggctgata tcctggctta tgtgacgttc cagtcaccgg
     2281 tgtatgtggc gatcttgtta gtggtgggcg cagactggca tcagattatg gcggcggtca
     2341 gttcaaacat cgttgtttcg atgttgatgg gggcggttta tggctacttc ctcgattatt
     2401 gccgccgact gtttaaagtc agccgttacc agcaggtaaa agcctgactc ttcttttcgc
     2461 gactggcatc gccagtcgcg tctttaactg gcttcgccaa ataaaccatt caaataacgt
     2521 tcaagcgcaa cacgcgaact gaagccatgc gggataccgt aatgctcgtg tcgctcgcct
     2581 gctaaataga gcggaaattg ctggtaatga tcgcaagttt taatatcgga agcgggttca
     2641 gccagtgttg aactgcgttc ttttaatgtt ggatgaatta ccagagcggt acgtcccatt
     2701 cgcgcttcgc gattaacata gacataattt tccccacggc ggtaaccata cgctttctgg
     2761 gttaccacat cgacagtaaa accgactttt tcaagtacgc gcgccacctc gtctggtcgt
     2821 aaatacatat ttgatcctcg ttattttcaa ccgcg
//
LOCUS       DAZPEB010000295         2812 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZPEB010000295 DAZPEB010000000
VERSION     DAZPEB010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.301"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            1..1555
                     /gene="cas3"
                     /locus_tag="ACHURJ_004089"
                     /pseudo
     CDS             1..1555
                     /gene="cas3"
                     /locus_tag="ACHURJ_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142534.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
     gene            1653..>2812
                     /gene="casA"
                     /locus_tag="ACHURJ_004090"
                     /gene_synonym="cse1"
     CDS             1653..>2812
                     /gene="casA"
                     /locus_tag="ACHURJ_004090"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HFR1282800.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAAWQFLLGLLQTSFAPKDQRSWDDIWEDGLEAEKLREALLSLEHAFQFGPDSPSFM
                     QDFDELKVKATFIASLLPDAPGKQTKERNTDHFIKRDTTQHLCLHCVPLALFSIQLNA
                     PIGGRGYYPGLRGGGPLTTLIELLEYQGNQQTPLWRKLWLNVMPQDEADLPLPKTFDD
                     LVFPWLAPTRTSELDGAVVTDEQVNKLQAYWGMPRRIRIDFKTTSIGNCDICGRQSDA
                     LLGLMSLKNYGVQYVMWRHPLTPYRLPLKEGGDFYSVKPQPGGLIWRDWLGLIEVGNS
                     KNNTELPAQVVKLLNASNLKQTRVGLWGFGFDFEDMKVRCWYEHHFPLLLNK"
BASE COUNT          704 a          626 c          774 g          708 t
ORIGIN      
        1 taatgaatca ctctggtccg gtgatttaat cgggtcagaa gaaccggatg aacaagcttt
       61 cagccagggt tgtgcggcct agtttgccga cagtaacaag aaggcgttgc tggctgaaat
      121 tggcgtcggc accctggatc aggcgatgat ggcagtgatg ccgtttaaac ataataatct
      181 gcggcttctg gggctgagta acaaaatcct gctggctgat gagatccacg cctgtgacgc
      241 ttatatgtcg tgcattcttg aagggctgat cgagcggcag gcgcgtggcg gaaacagcgt
      301 tattttgctt tctgctacgt tatctcaaca gcagcgcgac aaactcgtcg ccgcctttgc
      361 gcgtggtgca gagggccagc aagaagcccc actcctggga aaagatgatt acccctggct
      421 gacgcatgtc acgaaaaccg atgtacattc gcaccgggta gcgacgcgca aagaagttga
      481 acgtagcgtg agcgtgggtt ggctccatag tgaacaagag tgtattgcgc gtatcgaatc
      541 ggcggtaagt caggggaaat gcatcgcctg gattcggaat tctgttgatg acgctattca
      601 ggtgtatcgt cagctgcttg cccgcggcgt cattcctgct tccagccttt cactttttca
      661 tagccgcttt gcttttagcg atcgccagcg aattgaaacg gaaacgctgg cacgctttgg
      721 taaatattgt agtttacaga gagcgtctca agtcattgtt tgtacacaag taattgaaca
      781 aagcgtggat attgacctgg acgaaatgat ctccgacctg gcccccattg atttgctaat
      841 ccagcgagcg gggcgattac agcggcatat ccgcgatatt aatggtcagt taaagcgtga
      901 cggaaaagac gagcgttccc cccctgaatt gctgattctg gcccccgtct gggatgacgc
      961 tcctggtgac gaatggttcg gcagtgccat gcgtaacagt gcatatgtct atcccgatca
     1021 tgggcgaatc tggctgacgc agcgtgtgct gcgtgagcaa ggcgctattc agatgccgca
     1081 cgcagcccgg cttcttattg aatcagttta cggtgaggac gtggtaatgc cggaaggatt
     1141 tgcccgcagc gagcaggagc aagtgggcaa atattactgt gatcgcgcaa gggctaaaaa
     1201 gtatgtgctg aacttcaggc tcggctatgc cgccaatatc aacgattacc ttccggaaaa
     1261 actgtcgaca cgtctggctg aagagtctgt ttccctgtgg ctggctacct gtattgacgg
     1321 tgtggtgaag ccttatgcca ccggtgctca cgcatgggaa atgagcgttg tcagagtgcg
     1381 ccgaagctgg tggaaaaaac atcgggatga gttttcttta ctggaagggg atgcgttcag
     1441 gcagtggtgc gttgaacagc ggcaagatcc ggaaatggca aacgtgattt tagtcaccga
     1501 tgacgaaagt tgcgggtatt cggccaggga gggattgatt ggcaaggttg gttgagatgt
     1561 ctgcgacaag actctctgaa tagttgatat acagaaaagg atggaaggat ggacaaccga
     1621 cctgggatgg tcttttttac gagaggatgc gtatgaactc gttttcactt ctgacaaccc
     1681 cgtggttgcc cgttcgtttt aaagacggaa cgacaggcaa actggcacca gtcgatctgg
     1741 ctgatgaaaa tgttgtcgat atcgctgcgc cgcgggcaga tctccagggg gcggcctggc
     1801 agtttttgct ggggctacta caaaccagct tcgcgccaaa agatcaacgt agttgggatg
     1861 atatctggga agacggactg gaagctgaaa agctaaggga agcattgctg tctttagaac
     1921 atgcttttca gtttgggcca gattcccctt catttatgca ggattttgat gaattaaaag
     1981 ttaaggcaac ttttattgct tcgcttcttc ctgacgcacc aggaaaacaa acaaaagaac
     2041 gaaatacaga ccattttatt aagcgagaca caactcagca tctgtgcctg cattgtgtgc
     2101 ctttagcgct tttttcaatc cagttgaatg cacctatagg tggacgcggt tactatcctg
     2161 ggctacgtgg cggagggcct ctgacgacac ttatagaatt gctggagtat cagggcaatc
     2221 aacaaacccc tttgtggcga aagctgtggc taaacgtaat gccgcaagat gaagccgatt
     2281 tacctttacc aaaaaccttt gatgacctgg tctttccttg gcttgcaccg acgcgtacca
     2341 gcgaactgga cggtgctgtg gtaacggatg aacaagtcaa taaacttcag gcctactggg
     2401 gaatgccacg acgtatacgt attgatttta agacaacctc aataggtaac tgcgacattt
     2461 gcggtaggca gagcgatgct cttttgggct tgatgtcact caaaaactat ggtgttcaat
     2521 acgtcatgtg gcgccatcct ctaacacctt accgtctgcc gcttaaagag ggtggagatt
     2581 tttactcagt taaaccacaa cctggtggtt taatctggcg cgactggtta ggcctcatag
     2641 aggtaggtaa ctcaaaaaac aatactgaac tccccgcaca ggtcgtgaaa cttttgaatg
     2701 ccagcaatct aaagcagaca agggtgggct tatggggatt tggttttgat tttgaagata
     2761 tgaaggttcg ttgttggtat gaacaccatt tcccgctgtt gttaaacaaa aa
//
LOCUS       DAZPEB010000296         2784 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZPEB010000296 DAZPEB010000000
VERSION     DAZPEB010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.302"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..>2784
                     /gene="ehaG"
                     /locus_tag="ACHURJ_004091"
     CDS             <1..>2784
                     /gene="ehaG"
                     /locus_tag="ACHURJ_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trimeric autotransporter adhesin EhaG"
                     /protein_id="HFR1282801.1"
                     /translation="KLESLPTAPTYNLKNGSKNNVGAALAVLDENTLQWDQTKGKYSA
                     AHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVEANTANIATNTSNIATNT
                     ANIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQDTTSKITNVKDADLTADS
                     TDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLTETVTNLGEDALKWDKDN
                     GVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTNDAVATNTTNIATNTTNIS
                     NLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDGTVTATSSDAINGSQLYD
                     LSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALAAINSSFSTSLGDALLWD
                     ATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLHGVSSYVVDALGGGAEVN
                     ADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADAGENGAFSAAHGKDKTAS
                     VITNVANGAISAASRDAINGSQLYTTNKYIADALDGDAEVNADGTITAPTYTIANAEY
                     NNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITNVANGSISEDSTDAVNGS
                     QLNATNMMIEQNTQIINQLAGNTDATYIQENGAGINYVRTNDDGLAFNDASAQGVGAT
                     AIGYNSVAKGDSSVAIGQGSYSDVDTGIALGSSSVSSRVIAKGSRDTSITENGVVIGY
                     DTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQNAIGAVATTPTKYFHAN
                     STEEDSLAVGTDSLAMGAKTIVNGDKGIGIGYGAYVDANALNGIAIGSNAQVIHVNSI
                     AIGNGSTTTRGAQTNYTAYNMDAPQNSVGEFSVGSADGQRQITNVAAGSADTDAVNVG
                     QLKVTDERVAQNT"
BASE COUNT          780 a          725 c          684 g          595 t
ORIGIN      
        1 gtaagttgga gtcattacct acagcaccaa cctacaattt aaaaaatggt agcaaaaata
       61 acgtaggggc tgcgctcgct gtacttgatg aaaacaccct gcaatgggac caaaccaaag
      121 gcaaatacag cgctgctcat ggtactagta gcccaactgc cagcgtaatc accgatgttg
      181 cggatggcac gatttcagcc tccagtaagg atgcggttaa cggttcccaa ctgaaagcta
      241 ccaatgacga tgtcgaagcc aacaccgcca atatcgctac taataccagc aacattgcca
      301 cgaatacggc aaatattgcc accaatacca ccaatatcac caacctgacg gattccgttg
      361 gtgaccttca ggctgatgcc ctgctctgga acgaaactaa aaaggcattc agtgcagctc
      421 acggccagga taccaccagc aaaatcacca acgttaaaga tgccgacctg acggctgaca
      481 gcactgatgc tgttaacggc tctcagctga aaaccaccaa cgatgctgtg gcgacgaata
      541 ccaccaatat cgccaataac acttccaata ttgccactaa caccaccaac atctctaatc
      601 tgactgagac ggtgactaat cttggtgagg atgcgctgaa atgggataag gacaatggtg
      661 tattcacggc agctcatggc accgagacca ccagcaaaat caccaacgtt aaagatggcg
      721 acctgacgac tggcagcacc gatgccgtta acggctctca gctgaaaacc accaacgatg
      781 ccgtggcgac gaataccacc aatatcgcca ctaacaccac caacatctct aatctgactg
      841 agacggtgac taatcttggt gaggatgcgc tgaaatggga taaggacaat ggtgtcttca
      901 ctgcagctca tggcaacaat accgccagca aaatcaccaa tatcctggac ggcacagtca
      961 ctgcaaccag ttccgatgcc attaacggta gccagcttta tgacttaagc agcaatatcg
     1021 ccacctactt cggcggcaat gcttctgtga atactgacgg tgtgtttacc ggtccaacct
     1081 acaaaatcgg tgaaacaaat tattataacg tcggcgatgc actggctgcg attaactcct
     1141 catttagcac gtctctcggc gatgctctgc tttgggatgc caccgcaggt aaattcagtg
     1201 ccaaacacgg tactaatggt gacgcaagcg tgatcactga tgtcgcagat ggtgaaattt
     1261 cagactccag ttctgacgca gtaaacggct cacaactcca cggcgtgagc agttatgttg
     1321 ttgatgcgct ggggggtggt gccgaagtca atgcagacgg caccatcact gcgccgacgt
     1381 acaccattgc taatgctgat tacgataatg tcggtgatgc cctgaatgct atcgatacca
     1441 ctcttgacga cgctctgctc tgggatgcgg acgccggtga aaatggtgca tttagcgccg
     1501 ctcacggaaa agataaaact gccagtgtaa tcactaacgt cgctaacggt gcaatctctg
     1561 ctgccagcag agacgcgatt aacggctcac aactctatac caccaataag tacatcgctg
     1621 atgcgctgga tggtgacgca gaagtcaacg ctgacggcac catcaccgca ccgacttaca
     1681 ccattgcgaa cgccgagtac aacaacgtcg gtgacgctct ggatgcgctt gatgataacg
     1741 ccctgctgtg ggatgagact gccaatggcg gtgctggagc ctacaatgcc agccatgacg
     1801 gtaaagccag catcatcact aatgtcgcta atggcagtat tagtgaggac agtaccgatg
     1861 cagtgaacgg ttctcagttg aatgcgacga atatgatgat tgagcagaac acccaaatta
     1921 tcaatcagct cgctggtaac accgacgcaa cctatatcca agaaaacggt gcgggtatta
     1981 actatgtgcg tactaacgac gacggcttag cgttcaacga cgccagcgca cagggtgttg
     2041 gcgctacagc tataggttat aactctgtcg ccaaaggcga tagcagcgta gctattggtc
     2101 agggcagcta cagcgacgtt gatacgggta tcgccctagg tagcagctct gtttccagcc
     2161 gagtgattgc caaaggctcc cgtgacacca gcataacgga aaatggcgtt gttattggtt
     2221 acgacaccac ggatggcgaa ctgctcggtg cattgtctat cggtgatgac ggtaaatatc
     2281 gtcaaatcat caacgtagcc gatggttccg aagcccatga cgccgttacg gttcgtcaat
     2341 tgcagaatgc gattggtgcg gtcgcaacca cgccgactaa atacttccac gctaattcaa
     2401 cggaagaaga ttcactggca gtgggaactg actcgctggc aatgggtgcg aaaaccatcg
     2461 tgaatggcga taaaggtatt ggtatcggtt atggtgccta cgtggacgcg aatgcactta
     2521 acggcattgc cattggtagc aatgcgcaag tcattcatgt caacagtatt gcgataggta
     2581 atggttctac gaccactcgt ggcgctcaaa ccaattatac cgcctacaac atggacgcac
     2641 cgcagaactc tgtcggtgaa ttctcagtcg gtagtgcgga tggtcaacgt cagatcacaa
     2701 acgtcgcagc tggttcagcg gataccgatg cggttaacgt gggtcagttg aaagtcactg
     2761 atgagcgcgt agcgcaaaat accc
//
LOCUS       DAZPEB010000297         2751 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZPEB010000297 DAZPEB010000000
VERSION     DAZPEB010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.303"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..2192
                     /locus_tag="ACHURJ_004092"
     CDS             <1..2192
                     /locus_tag="ACHURJ_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFR1282802.1"
                     /translation="RYEKGGKDGITMTFIGPSPSVQDKSASTDNTNINGEDMTEIEES
                     MLLPVSGQELPIRWLAQHGSEKPVTHVSRDELQALHIARAEELPAVTALAISHKTSLL
                     DSLEIRDLHKLVRDTDKVFPNPGNSDLGLITAFFEAYLDADYTVRGLLTKEWMKGNRV
                     SRITRTASGANAGGGNKTDRNPNLVHTLDTLDVEIAAATLPMDFNIYEIPGSVYRRAK
                     EVVLKKESPFKEWSAALRAPPGILDYSRAAIFALIRSAHPEFYHYPGRLQGYINAYLT
                     ETDHENPSKETLTATRHTPEKDILEEINREVVTERETEEEKPQPSDAMAGEQATTETM
                     EPDTTEHCQNAQSLDAQSQVSSANQVKVTADEVNKIMQAANISQPDADKLLAVSRGEF
                     VEGISDPNDPKWVKGIQTRDSVNQNQHESERNDQKAEQNSPNALQNEPETKQSEPVAQ
                     QEPEKVCTACGQSGGGNYPDCGAVMDDATYQKTFDEENQVEVQENDPEEMEGAEHPHK
                     ENAGSAQDHASDSKTGETADPLITVNGHHEITSTSRTCDHLMIDLETMGKNPDAPIIS
                     IGAIFFDPQTGDMGPEFSKTIDLETAGGVIDRDTIKWWLKQSREAQSAIMTDEIPLDD
                     ALLQLREFIDENSGEFFVQVWGNGANFDNTILRRSYERQGIPCPWRYYNDRDVRTIVE
                     LGKAIDFDARTAIPFEGERHNALDDARYQAKYVSAIWQKLIPSQADF"
     gene            2251..2454
                     /locus_tag="ACHURJ_004093"
     CDS             2251..2454
                     /locus_tag="ACHURJ_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFR1282803.1"
                     /translation="MSSLFLTEDELLILTGCKYASHQRKWLMENELPFYTNRSGKPIV
                     SRDLFTCNKTLPPREVEPNFGAI"
     gene            2454..2751
                     /locus_tag="ACHURJ_004094"
                     /pseudo
     CDS             2454..2751
                     /locus_tag="ACHURJ_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310800.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT          842 a          665 c          652 g          592 t
ORIGIN      
        1 accgctatga aaaaggcggg aaagatggca tcacaatgac atttatcggc ccttccccct
       61 ctgttcagga caaatcagcc agcactgata ataccaacat caacggcgaa gacatgactg
      121 agattgagga gagcatgctt ctacctgtct ccggtcagga actgcccatt cgttggcttg
      181 ctcaacacgg cagcgaaaaa ccagtaacgc acgtttcacg cgacgaactc caggcattac
      241 acattgcacg ggctgaagaa ctaccggctg ttactgccct ggctatttcg cataaaacca
      301 gtctgctcga ctcgctggag attcgcgacc tccacaaact ggttcgtgac actgacaaag
      361 ttttccctaa tcctggtaat tcagacctgg gactaataac tgcttttttc gaagcatact
      421 tggacgctga ctacactgtt cggggcctgc tgacaaaaga gtggatgaaa ggaaatcgtg
      481 tttcacgcat cacccgcacg gcttccggtg ctaatgctgg tggtgggaac aaaaccgatc
      541 gcaatccgaa tttagtacac accctcgaca cactggatgt ggagattgca gcagccacac
      601 ttccgatgga ttttaatatt tatgaaattc cgggcagcgt ttatcgtcgc gcaaaagaag
      661 tagtcctgaa aaaagaaagt ccgttcaaag aatggtccgc agcacttcgt gcacccccgg
      721 gtattctgga ctattcccgc gccgctattt ttgcacttat ccgaagcgca caccctgaat
      781 tttatcacta cccgggacgc cttcaggggt atatcaacgc ctatttgacg gaaactgatc
      841 acgagaaccc cagcaaggaa actctcacag ctacccggca tacgccggaa aaagatatcc
      901 tggaagaaat taaccgcgag gtggttactg agcgtgaaac agaagaagaa aaaccacaac
      961 catctgacgc aatggcaggt gaacaggcaa caactgaaac aatggaaccg gatacaactg
     1021 aacattgcca gaacgcgcag tcgctggatg ctcagtcgca ggtgagttcc gctaaccaag
     1081 taaaagtcac cgctgacgaa gtaaacaaaa ttatgcaggc agccaatatc agccagcctg
     1141 acgccgataa gttacttgct gtatcgcgtg gtgaatttgt tgaggggatt agcgacccta
     1201 atgatccgaa atgggtcaag gggatccaga ctcgcgattc tgtgaaccag aaccagcatg
     1261 aatcggaacg gaacgaccaa aaagcggaac aaaacagccc aaatgcgtta caaaacgagc
     1321 cagaaacgaa acaatccgaa ccagtagcgc aacaggaacc ggaaaaagtc tgcaccgcct
     1381 gcggtcagag cggtggcggc aactaccctg attgtggcgc ggtgatggac gacgcaacat
     1441 accagaaaac attcgatgaa gagaatcagg ttgaagttca ggaaaatgat ccggaggaaa
     1501 tggaaggcgc tgaacatcca cacaaggaga atgctggcag cgctcaggac cacgccagcg
     1561 atagtaaaac tggcgagacg gcagatccct taattacggt gaatggtcat cacgaaatca
     1621 catccaccag caggacgtgt gaccatctaa tgatcgacct tgaaaccatg ggaaaaaatc
     1681 ctgatgcccc gatcatctca ataggtgcaa tatttttcga tccgcaaacc ggagatatgg
     1741 gacccgaatt tagtaagact atcgatctgg aaactgctgg cggagtcatt gatcgggaca
     1801 ccattaaatg gtggcttaag caatcacgcg aagcgcaatc tgccattatg accgatgaaa
     1861 tcccgttaga tgatgcactg ttacaattgc gggaatttat cgacgaaaac tccggtgaat
     1921 tttttgttca ggtctgggga aatggagcca acttcgacaa cacgattttg cgccgttcat
     1981 acgaacggca ggggatcccc tgcccgtggc gttactacaa cgatcgcgat gtacgcacaa
     2041 tcgttgagct ggggaaagcc atagacttcg atgccagaac ggctattcca ttcgaaggtg
     2101 agcgccataa tgcacttgat gacgctcgtt accaggcaaa atacgtttca gctatctggc
     2161 aaaaactgat cccgagtcag gctgattttt aatgttcaac cctaattgcc gctaaccgta
     2221 tatagttagc ggcggttatg agatatagct atgagcagct tatttttaac cgaagatgaa
     2281 ttgctaatat taacgggctg caaatatgca agccaccagc gaaaatggtt aatggaaaac
     2341 gagcttccgt tctataccaa tcgtagtggc aaaccgattg tcagccggga tctatttacc
     2401 tgcaataaaa ctttaccacc acgcgaggta gagccgaatt ttggtgcgat ctgatgggaa
     2461 gacgaaggaa aaatcctgag cacgaaaaat tacctccaaa tgtataccca aataaatata
     2521 gttatgtatg gaaaccaaca tccagcgaat ctgtaacact aaccgccatc aaggatggtt
     2581 tagctgcttt atggaaaaag tatgaggaaa ctgtaaataa tcgcgatcgt gcaatgacat
     2641 tcggtcgctt gtgggaaaaa ttcctcgcca gcgcctatta cagtgacctc agtccaagaa
     2701 cacaaaaaga ttatctgcaa catcaaaaaa gttgcttgcc gtattcggta a
//
LOCUS       DAZPEB010000298         2741 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZPEB010000298 DAZPEB010000000
VERSION     DAZPEB010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.304"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..173
                     /locus_tag="ACHURJ_004095"
     CDS             <1..173
                     /locus_tag="ACHURJ_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="acts in anaerobic beta-oxidation of fatty acids;
                     possible ligase/synthase involved in anaerobic growth on
                     fatty acids; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclohexanecarboxylate-CoA ligase"
                     /protein_id="HFR1282804.1"
                     /translation="EVVAFFSRKRVAKYKYPEHIVVIEKLPRTASGKIQKFLLRKDIM
                     RRLTQDVCEEIE"
     gene            complement(230..2608)
                     /gene="ppsA"
                     /locus_tag="ACHURJ_004096"
     CDS             complement(230..2608)
                     /gene="ppsA"
                     /locus_tag="ACHURJ_004096"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFR1282805.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNGRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
BASE COUNT          654 a          761 c          683 g          643 t
ORIGIN      
        1 aagaggtggt ggcttttttt agccgtaaac gggtcgcaaa atataaatat cctgaacata
       61 tcgtggtaat cgaaaaacta ccgcgcactg cctcaggtaa aatacaaaag tttttgttaa
      121 gaaaagatat tatgcggcga ttaacgcagg atgtctgtga agagattgaa taagtttcat
      181 cttcggggat cacataaacc cggcgacaaa acgccgccgg gtatttattt tatttcttca
      241 gttcagccag gcttaaccag gtttgcacca cggtgtccgg attcagagac aggctatcga
      301 tcccctcttc catcaaccat gcggcaaaat cttcgtggtc ggacggaccc tgaccgcaaa
      361 tcccgacata tttgccctgt ttcttcgcgg cacggatagc catcgacagc aatgctttca
      421 ccgcatcgtt gcgctcatcg aacagttcag acaccacgcc ggagtcacga tccagaccga
      481 gcgccaactg cgtcatgtcg tttgagccaa ttgagaagcc gtcgaaatat tcgaggaact
      541 gctcggccag caaggcgttg gacgggattt cacacatcat gatgattttc agcccgttct
      601 caccacgctt cagcccctga cgcgccagtt cttcaaccac cgctttcgcc tggtctacgg
      661 tacgcacgaa cgggatcatg atctcaacgt tggtcagccc catgtcgttg cgcacacgtt
      721 tcactgcttc acactccagc gcgaagcagt cgcggaagct gtcggaaaca tagcggcccg
      781 cgccacggaa gccgagcatc gggttctctt catctggctc gtaacgctca ccaccgacca
      841 ggttggcata ttcgttcgat ttaaaatcag agagacggac aatgacgcgc ttcggataaa
      901 acgcggcacc cagcgtcgcg atcccttcag tcagacgacc aacgtaaaat tcacgcggag
      961 aatcaaaacc tttcatcatc tcgcggattt cgttttgcaa ctgcggttcc tgatcgtcaa
     1021 actcaagcag tgcgcgtggg tggacgccaa tcatacggtt gatgataaat tccagacgcg
     1081 caagtcccac gccttcgttc ggcagacagg cgaagtcgaa agctcgatcc gggttaccga
     1141 cgttcatcat cactttcaac ggcagatccg gcatggtttc tacgctggag cttttcacgc
     1201 taaattccag caactccgca tagacgtaac cggtatcacc ttcggcacaa gaaacagtga
     1261 cgttctcacc gtctttcatc cgttctgttg catcaccaca gcccacgacc gccggaatac
     1321 ccagttcacg agcgatgatc gccgcgtgac aggtacgacc gccacggttg gtgactatgg
     1381 cagatgcttt cttcatgatc ggttcccagt ccgggtcggt catgtcagtg accagcacgt
     1441 caccaggttc gatgcggttc atttcgctga tgtcatggat gactttcacc ggacccgcac
     1501 cgatgcgatg accgatagca cggccttcgg cgataatctt accctgtgaa tgcagcgtat
     1561 aacgctccat gacctgaccg cgtgagcgca cggtttccgg acgcgcctgc acaatgaaca
     1621 gtttgccggt gtggccatct ttcgcccact caatatccat cgggcgaccg tagtgtttct
     1681 caatttgtac ggcctgtttt gccagttcct gcacttcttc gttggtcagc gagaagatgt
     1741 cacgctgttc ctgcggtacg tcttcgattt taacctgctt gccgtgctcc tgggtcggcg
     1801 cgtaaaccat gcggattttt ttcgacccca tggtgcggcg cacgatagcc gggcgattcg
     1861 ccgccagtgt cggtttatgc acgtaaaact catccgggtt aaccgccccc tgcacgacca
     1921 tctcaccaag gccccatgcg gaagtgataa acaccacctg gtcaaagccg gattcggtat
     1981 caatggagaa catcacgcca gaagatgcga ggtcggagcg caccatccgc tgaacaccgg
     2041 cggagagcgc cacgccacgg tgatcgtaac cctggtgcac acgataagag atggcgcgac
     2101 cgttaaacag agaagcaaat acatgtttca ctgccacgag aacggcgtca aacccctgaa
     2161 cgttgaggaa ggtttcctgc tgaccggcaa aggaagcgtc cggcatatct tctgcggtgg
     2221 cggaggagcg caccgcaaaa gaggcgtttt cgtcatcggc ggaaagctga gcataggctt
     2281 cgcggatggc gttttccagc tcaggctgga agggagtgtc gataatccac tggcggattt
     2341 gcgcgcccgc tttcgcaagc tgagtaacat cgtcaatatc cgttttatcc agcagttcat
     2401 aaatgcgctg gtttacgccg ctttggtcca gaaactggtt aaacgcgtcg gcggttgtgg
     2461 cgaaaccatt cggaacggaa acgcccattc cggaaaggtt agtaatcatt tcacccaggg
     2521 aggcattttt gcccccaacc ctgtctacat cattcatgcc gagttggttg taccaaagca
     2581 ccagcggtga cgagccattg ttggacatcg aacaatcctt ttgtgataaa tgaacggttt
     2641 gagaaacaca tttctgcgca tttatctttg catatttaac cggatgaaaa aaacggtgaa
     2701 tcgttcaagc aaatatattt ttttactttt taagactgat c
//
LOCUS       DAZPEB010000299         2719 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZPEB010000299 DAZPEB010000000
VERSION     DAZPEB010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.305"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(269..1048)
                     /gene="ipaJ"
                     /locus_tag="ACHURJ_004097"
     CDS             complement(269..1048)
                     /gene="ipaJ"
                     /locus_tag="ACHURJ_004097"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HFR1282806.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(1245..2055)
                     /locus_tag="ACHURJ_004098"
                     /pseudo
     CDS             complement(1245..2055)
                     /locus_tag="ACHURJ_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            2402..>2719
                     /locus_tag="ACHURJ_004099"
     CDS             2402..>2719
                     /locus_tag="ACHURJ_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1282807.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSV"
BASE COUNT          798 a          687 c          454 g          780 t
ORIGIN      
        1 caattacaac gagtgactaa aataaaaata tcatccaagt caatcactgt ttgctctgca
       61 aaatatgtca gccttctcaa acttaccagc acttaaatat ttaagcacac aatcattcct
      121 tgagggagtt cacttatttc aagagaaaca tttcgtcttc ccacacaact aatccgccca
      181 atatagctaa atttgtactc agtaatatca tttgttatgc cccccgcccc aaaatattca
      241 agctccttca cagataaaaa acgacaattt acaaagcctc attagttata actatggaaa
      301 tacctatttt agtatatcct ataaagttag aattacttct tcttgcacct aattccatag
      361 ttgaaaaaca agaataattt tctcctacag ctggatccat atagcttcca tcaggtctac
      421 acaacaccca gtggaggccg acaggaactc caataaagga tacagcaaca gcttccaaaa
      481 ctctttggtt agaagataac acatctcgtt gatgtactat attacatccc atgccaatta
      541 ataagtccct tgcatcggga taaataaaac taattacttg cgaaaaaatg ttactttctt
      601 caacgacata agcattcaat ccaagaagcc tcgtagccat gactatctta tcaggcatag
      661 aataaccggc atccgcgata ttatctttgt gtattctcgg attataatta ccactagtta
      721 ttaagtataa atcccgctca caacgattat ccaaatcaag agaactcttc cttgtcattt
      781 cggacataga ccctttgtat tgtggtattt tatcaacccc taattccttt gcagcacaga
      841 gtaaactggc agctccacaa gaattgtcat acatctgctt cctcgccaaa aaacaacctt
      901 gttcgtttac tctattctca tttaccctca catcagtctg atgagatatc atatattctc
      961 ttggtatagc cccctgcaat aaaaccccat ataacataac acctgtatga atacatcccc
     1021 gtttgcaagg cttccgttgt tccgacatcg acgagtaatt gcagcacagt attgcatcca
     1081 gcaatgcgaa ctactatgag aattagctga ccgggtcctt attccccgaa cgaactttaa
     1141 atccaaaacc tcatactctt ttccgtcgtc atcacgtccg tcaccataac aacctttcag
     1201 catccaccct tttattaaga gttaatgtcc atcagcatca gcaattaata ccgcctgata
     1261 ctgacatatt tttgtcgtat ttacgtccat aatcggttat tgtgatacag gtcacaccag
     1321 cttatttgct attgttacaa taaccacctt ggttgagagc tgttttttca actttgttat
     1381 caactccccc aactccttgg tcagtaaggc tgccgaccga gcacagtgaa tagatagtat
     1441 ccgcaggtac caccatgatt acttaatact cagaaggcga agtgctctcc aattaactct
     1501 gccgcactat gcttataagc tgcttttcaa tgccgtctat caacatgtcc agttcattca
     1561 atctgttgta ctggtaatcc aacgcagtaa tgatatatgc aggtattctt tcatccactc
     1621 cagtaacgtt tcatgcaaag cattaattta cgcgctacgg aacttcacca gttgactgag
     1681 caaacaatgt gggaccagta ctgcctgcag ttcttctatt gttacagcga tttctttacc
     1741 aaacagctga accaccatca agataaagat agcctgaaca ttcatcgcac cattttgtta
     1801 ccaatggcaa aagatttaac gaaccgtgcc tgcatcaggc ggacttttta tccctgcttt
     1861 gttagctccc taaccttgta cagagagcct ccataagctc ccatatcaag cagacatggt
     1921 tctgcagtta ctgacataca ccagaaaact ctgacagcag actcacttat cctccacttc
     1981 actcatatcc ttattggtga gatgaacctg aatgacatgt tttgcgacat aaacgccaac
     2041 cagtgtatat ttcacctgta gagattctac aatctgtagc gttatgcatc taatgttagg
     2101 ctctatgatg tcagaaatct gcgagactct acaccttctg catttacctt tatttatcac
     2161 catgctcctc agttaacgga gaacgtgttc attgcatttt gtcacttaca tttcgccttt
     2221 ggtatcgggg ggatataggt tccaacgttc cgcatgagaa cctgaaccgg cttcacgcca
     2281 ccttgatacc agatgccgca caggcagtca acaagtgtca cgaacggtgc aatagtgatc
     2341 cacacccaac gcctgaaatc agatccaggg ggtaatctgc tctcctgatt caggagagct
     2401 tatggtcact tttgagacag ttatggaaat taaaatcctg cacaagcagg gaatgagtag
     2461 ccgggcgatt gccagagaac tggggctctc ccgcaatacc gttaaacgtt atttgcaggc
     2521 aaaatctgag ccgccaaaat atacgccgcg acctgctgtt gcttcactcc tggatgaata
     2581 ccgggattat attcgtcaac gcatcgccga tgctcatcct tacaaaatcc cggcaacggt
     2641 aatcgctcgc gagatcagag accagggata tcgtggcgga atgaccattc tcagggcatt
     2701 cattcgttct ctctcggtt
//
LOCUS       DAZPEB010000300         2683 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZPEB010000300 DAZPEB010000000
VERSION     DAZPEB010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.306"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            281..1123
                     /locus_tag="ACHURJ_004100"
     CDS             281..1123
                     /locus_tag="ACHURJ_004100"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFR1282808.1"
                     /translation="MLNGGGILFKANHVPVLMYHHVSHCPGLVTLSPETFRKQMKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLNEFNLKAHIFLIT
                     SFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKLTSREEQCKHLRQDLLSGREYLKKMTGKCSKHLCWPEGYYNKDYIQIAEELG
                     FHYLYTTERRMNAPAKGTTRIGRISTKERENCVWLKRRLFYYTTPFFSSLLALHKGPR
                     LPDD"
     gene            1126..2214
                     /gene="capU"
                     /locus_tag="ACHURJ_004101"
     CDS             1126..2214
                     /gene="capU"
                     /locus_tag="ACHURJ_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HFR1282809.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKFGIDITFALFRNSLHIPTVWRLLGIVHSFQPDAIVCHSGHDSNIVGLVRFFTW
                     KHPFRIIRQKTYLTRKTKFFSINHFCDEVIVPGTNMKTHLEQEGCRTRVTVVPPGFDF
                     QELYVDSRNSLPPSVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVVATQIGGIPEVIQNNQTGTLLPAGNKHAWMCALNNFFNDPGRFYQM
                     ARLAKQDIEERFDINKTALKILTLAKQK"
     gene            2219..>2683
                     /gene="virK"
                     /locus_tag="ACHURJ_004102"
     CDS             2219..>2683
                     /gene="virK"
                     /locus_tag="ACHURJ_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HFR1282810.1"
                     /translation="MFSISNLSFIGFLKRIIFSSDSLPGKWEHRKFRFMYILRCSINP
                     VVSIRYYYELRSLPCIEDILAIHPTLPARIHRPYLHKGGRAWTRGQYILEHYRFVQNL
                     PEKYSKFLFPQKSVSLVQFIGKDGENFDIQCSPSGFDREGELMLSLFYNKTVI"
BASE COUNT          771 a          484 c          589 g          839 t
ORIGIN      
        1 ttctggtcaa caaccagttg agcggattcg cgtttaaact ctgcgctgaa atttcttttt
       61 tcattatggc acctgtgttg ttctgaggtg agcatatcac ctctgttcag gtagctaaat
      121 ttagtgtgcc acctcaaggt tcttagtgag ccgtttttaa aagatatgat aatatattat
      181 tgtttataaa ttgttgatgt ttgatttagt agtatataaa gttcaccaac tttattaggc
      241 tttcctttta tctctactta ctgggtgcca gaaactttaa attttaaacg gagggggcat
      301 tttgtttaaa gcaaatcatg ttccggtatt aatgtatcat catgtcagcc attgcccagg
      361 actggttaca ctttctcccg agacattccg gaaacaaatg aaatggttgg ctgaaaacaa
      421 ctggaaaaca ctgtcatcag atgaactgga gtttttttat cggggcggaa aacttccccg
      481 taaaagcgtt atgttgacat ttgatgatgg ataccttgac aactggtttc aggtatatcc
      541 tttattaaac gagtttaatc tcaaggcaca tattttttta attaccagtt ttattggaaa
      601 tggtccggtt cgtcactctc ctggaaagga atattctcat cgcgattgcg aacatcaaat
      661 agcaaccggg aatgctgata atgttatgct tcgctggtct gaagtgaacg aaatgttgca
      721 atctggtctg gtcgaatttc atgttcatac acatactcat acacgttggg ataaaaagct
      781 cacttcacga gaagaacagt gtaaacatct tcggcaggac ttattgagcg ggagagaata
      841 tttaaaaaaa atgacaggaa aatgcagtaa acatttgtgt tggccggaag gttattataa
      901 caaggattat attcagatcg ctgaagaact tggattccat tatttatata caacagaaag
      961 aagaatgaat gctcctgcta aaggaactac acggatagga agaataagca ccaaggaacg
     1021 ggaaaattgc gtatggctga aaagacgact tttttattat acaacaccgt ttttttcatc
     1081 gttgctggcc cttcataagg ggccaagatt accggatgac taataatgaa tatactattt
     1141 acggaatcat caccgaatat aggtggtcag gaattacagg ctgttgctca aatgaaggcc
     1201 ctgaagaaaa tggggcattc agttctgctt gtctgcaggg agaacagcaa aattgctttt
     1261 gaagccagta agtttggaat tgatatcaca ttcgcgttat ttcgaaacag tcttcacatt
     1321 cctactgtat ggagattact cggaatagtt catagttttc agcccgatgc aatcgtttgt
     1381 cacagtggac atgatagtaa tattgttggt ttggtacggt tttttacctg gaaacatcca
     1441 ttcagaatta tcaggcaaaa gacatatttg acacgaaaaa caaaattttt ttcaataaat
     1501 catttttgcg atgaggtgat tgttcccgga acaaatatga agacacatct ggagcaggaa
     1561 ggatgtcgaa ctcgggttac tgttgtgcct cctggctttg atttccagga attatacgtt
     1621 gattcccgaa acagtttgcc tccgagtgtt ctttcctggc tggcatcccg aagaggatgc
     1681 cccgtaattg ctcaggtagg aatgttgcgc ccggaaaaag ggcacgaatt tatgttgaat
     1741 ttactgttcc atttaaaaat gaatggacga cagttctgtt ggttgattgt ggggtctggt
     1801 tcacctgaac tgcgggaaca tttacagtat cagattgaca gtatgggcat gcatgatgat
     1861 gtttttattg ctgacaatgt ttttcctgcc gcccccgtat atcgggttgc cagtctggtg
     1921 gttctgcctt cagaaaacga atcttttggt atggtgctgg cagaagcatc ggcattttct
     1981 gtgcctgtag tggccactca gattggtgga atccctgagg ttattcagaa caaccagacc
     2041 gggacattgt taccagcagg taataagcac gcatggatgt gcgccctgaa taattttttt
     2101 aatgaccctg ggcgttttta tcagatggct cgcctggcaa aacaggatat agaagagcgg
     2161 tttgatatta ataaaactgc gttaaaaata ctcacattag cgaagcaaaa gtaacaatat
     2221 gttttctata agtaacttat catttatcgg tttccttaaa aggattattt tttcctcaga
     2281 ttcactaccg gggaagtggg aacacagaaa atttcggttc atgtacattt tgcgatgttc
     2341 tataaatccg gttgtcagta ttcgatatta ttatgaactg cgttccttgc cgtgcattga
     2401 ggatattctg gcaatacacc ccacgttgcc agcaagaatt catcgccctt atttgcataa
     2461 aggggggcgc gcctggaccc gtggacaata tattcttgag cattatcgct ttgtgcaaaa
     2521 tttgccagaa aaatattcta aatttttgtt tccacaaaaa tcggtatcat tagtccaatt
     2581 catcgggaaa gatggagaga attttgatat ccagtgttct cccagtggtt ttgaccggga
     2641 aggagagttg atgttgtcat tgttttacaa taaaacagtt att
//
LOCUS       DAZPEB010000301         2675 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZPEB010000301 DAZPEB010000000
VERSION     DAZPEB010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.307"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(48..232)
                     /locus_tag="ACHURJ_004103"
                     /pseudo
     CDS             complement(48..232)
                     /locus_tag="ACHURJ_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311258.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
     gene            complement(394..468)
                     /locus_tag="ACHURJ_004104"
     tRNA            complement(394..468)
                     /locus_tag="ACHURJ_004104"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(433..435),aa:Arg,seq:cct)
     gene            complement(544..1476)
                     /locus_tag="ACHURJ_004105"
     CDS             complement(544..1476)
                     /locus_tag="ACHURJ_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFR1282811.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETHDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            1770..2525
                     /gene="mlaA"
                     /locus_tag="ACHURJ_004106"
     CDS             1770..2525
                     /gene="mlaA"
                     /locus_tag="ACHURJ_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFR1282812.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT          651 a          634 c          654 g          736 t
ORIGIN      
        1 atcttttacg gggtgaaggt agatttcgta tgaagggtat ctgttgtctc ctgcaaagtc
       61 ataagcggat attttcctac ggtcagtatt tttttcttac cgtcaatctt gtagcgaagt
      121 tgccatacct tttcccggat acaggaacat aaaggtacag gccattacca tcgagaaggc
      181 ggtatggttt ttctttcggc tttgctgctt caatctgctt cacggtgagc atgggtaaaa
      241 atccggtggg taaaattatt ttatccactt tttacccgtc atggagtgcg gctgtcaacg
      301 atctgacgcg aaccatgacg aaccgtgaat atacggaagg cttgatattc aggggatttt
      361 gcggactggt acggatggga gcgaactgat aaatggtgtc ccctgcagga atcgaacctg
      421 caattagccc ttaggagggg ctcgttatat ccatttaact aagaggacaa tgcggcatga
      481 gtatacccgc taatggactg cggagtaagt acactgccgc tcgattgctt aaaccctcgc
      541 catttatgct gggtttttat catttttctt aatgttttcc gcacgttctg ctttttggcg
      601 tgcttctgct ttgcgcttgt tgctcatgtc gttacggatc tgtgcatgac tcattaacgc
      661 gaagataaag gtgccgccgc agatattccc cgctaaagta ggtagtgcga agggccaaat
      721 gaaatcgctc cagtgcagcg tgccgttaaa caccagatag aggatttcta cagaaccgac
      781 aacgatgtgg gtggtgtcac ccagtgcaat aagccaggtc atcaatataa tcaccacaat
      841 ctttgccgca cccgctgcag gaaacatcca aaccatagtg gcgatcagcc agccggaaat
      901 gatcgcgttg gcaaacatct cgctgggggt gttcttcatc acatccatgc cgattttgac
      961 aaatgcatcg tgagtttctt cattgaagat aggcatatat tcaaatgccc atgcagcaat
     1021 acctgtcccg agaatattac ccagcagcac gacgccccat aatcgcataa gtaagccgac
     1081 gttgctcatt gtcggttttt gcatgacggg tagtaccgca gtcacggtgt tttcggtaaa
     1141 taattgctgg cgggccataa tgacgataat aaaaccaaag gtataaccga gattctccaa
     1201 caagaagctg cccggcacgc cttccagttc gacatgaaat atcccttttg ccagtaacga
     1261 agcgcccatc gacagacccg ccgcaatggc tgaccacagt agtgccattg cgtcgcgttc
     1321 cagctctttt tcaccatcct ggcggatatg ttcatgaatt gccatcgccc gggaggggag
     1381 tcgatcttca tctatttcta tttttttgcc gcgctctttt tcttcgctct caacttcaat
     1441 ttcgtcgctg tgttgatcaa ttttgtcgtt gtccatggtc tcttctcttg aattagcacg
     1501 tatagctaaa gcgtagcggc ttttttgctc gcaactggcg ggagttactc tgaaaatgta
     1561 gaaaaggccg cgtttgcctt tttctgtttc tatagaatca agtagcctac agggcggcga
     1621 ttaccaggct atgatcaaat cagcaaatca gggcgtctgg acatcagttg acgtgctgtt
     1681 acaatcgccc acacctaaac aggcggatac ggtatcgttc cgtcatggat ggcaaactgc
     1741 ataagccata aaaaaacagg gagacattta tgaagcttcg cctgtcggcg cttgctctgg
     1801 gaactacgct tctggtgggg tgtgcgagtt ccggtacaga tcagcaaggg cgttctgacc
     1861 cgttagaagg gttcaaccgc accatgtaca acttcaactt caatgtatta gacccgtata
     1921 ttgttcgacc ggtcgctgtc gcctggcgtg attatgttcc gcaaccggcg cgtaacggtt
     1981 tgagcaactt taccggcaac cttgaagaac ctgcggtgat ggttaactac ttcttgcagg
     2041 gcgaccctta tcaggggatg gtccacttta cccgcttttt cctgaacacc attttgggga
     2101 tgggcggctt tattgatgtt gcagggatgg cgaacccgaa actgcaacgg actgaacctc
     2161 accgcttcgg tagtacgctt ggtcattatg gcgtgggtta tgggccttac gttcagttac
     2221 cgttctacgg tagcttcacg ctgcgtgatg acggtggtga tatggcggat ggtctttacc
     2281 cggttctttc ctggctgacc tggccgatgt ctgtgggtaa atggacgctt gaagggatcg
     2341 aaacccgcgc tcagctgctg gattccgatg gtctgctgcg tcagtcgtcc gatccttata
     2401 ttatggtgcg cgaagcgtac ttccagcgtc atgatttcat cgctaatggc ggcgaactca
     2461 aaccgcagga aaatccgaac gcacaagcga ttcaggatga tttaaaagat attgattctg
     2521 aataagaaac aaataaaaaa ggtgagtcgc aaaactcacc ttttttgttg ttatccctca
     2581 attaccacaa gtaaaaggag ataaaccgac aatgacccgg acgtaatgat gtaaaaatac
     2641 tgatgttcgt cactgactat ttccgatatt caggc
//
LOCUS       DAZPEB010000302         2668 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZPEB010000302 DAZPEB010000000
VERSION     DAZPEB010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.308"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            45..770
                     /locus_tag="ACHURJ_004107"
     CDS             45..770
                     /locus_tag="ACHURJ_004107"
                     /inference="COORDINATES: protein motif:HMM:NF045398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adhesin"
                     /protein_id="HFR1282813.1"
                     /translation="MKILNSNLITNISSTIRNHLLTSSKNIKTHPSPNMDVTSHRRLF
                     QKDGIGLSQTGKATQLVIMSHGGWKEISHPQTLFTRQKGDGWTVVPKNLRIDFYTKDN
                     DFTKGLSVLSEVNKRHTEAQKGLTPSLNISKDDLKVLANARNIPQSKLEEEMMSQAIY
                     RKEYATSNEKIKNYALYYHEQAQNIIQRHQDGLGNKNIDIAFITDKNHKKHLSDIFKV
                     INESGVKYDVIHFGACRVNREEK"
     gene            complement(996..1319)
                     /gene="mobA"
                     /locus_tag="ACHURJ_004108"
     CDS             complement(996..1319)
                     /gene="mobA"
                     /locus_tag="ACHURJ_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="HFR1282814.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLR
                     AAALGKNVNSLTDDRVLKEVMRLGALQKKLFIDGKRIGDREYAEVLIAITEYHRALLS
                     RLMAD"
     gene            1592..1786
                     /locus_tag="ACHURJ_004109"
     CDS             1592..1786
                     /locus_tag="ACHURJ_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFR1282815.1"
                     /translation="MNKQQQAVLNMAGFIKSQSLTLLEKLDALDADEQATMCEKLHEL
                     AEELQNSIQTRFEVENSTER"
     gene            2274..2435
                     /locus_tag="ACHURJ_004110"
     CDS             2274..2435
                     /locus_tag="ACHURJ_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR1282816.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHELSPLAAATDNRIRGVRL
                     EVMRLLRLN"
BASE COUNT          863 a          656 c          564 g          585 t
ORIGIN      
        1 aaaaaaaaac aataaaaaac accgcaaaaa tggattgtta acaaatgaaa atactcaatt
       61 cgaatctcat aacaaacata tcatcaacaa taagaaatca ccttctaaca tcatccaaaa
      121 atataaaaac acacccatca ccaaacatgg acgtaacaag tcatcgtcgt ttattccaga
      181 aagacggaat aggattgagc caaacaggaa aagcaacaca acttgtcatt atgtcgcatg
      241 gtggatggaa agagatctcc catccacaaa cactattcac gcgtcaaaaa ggcgatggat
      301 ggactgtagt ccccaaaaac ctaagaattg atttttatac aaaagacaac gacttcacaa
      361 agggactaag cgttctttca gaggtaaaca aacgccatac tgaagctcaa aaagggttaa
      421 caccatcact aaacataagc aaagatgacc taaaagttct agcgaacgcg cgcaatatac
      481 ctcaatcaaa attagaagaa gaaatgatgt cacaagccat atatagaaaa gaatacgcaa
      541 caagtaatga gaagataaaa aactatgctc tatattacca tgaacaagcc caaaatatta
      601 tacaaagaca tcaagatggc ttaggaaaca aaaatattga tatagcattt ataactgata
      661 aaaatcataa gaagcacctt tctgacatat ttaaagtaat aaatgaatca ggagtaaaat
      721 atgatgtaat tcactttggc gcttgccgag taaatagaga ggagaaataa tcatcattta
      781 ccataatccc ttaattgtac gcaccactga aacgcgctga acgcgattct gacgcagaca
      841 ccacaaaaat agatgtttta ccccggaaaa acaggaagga tgcgtcagga tcgttttcag
      901 ctcgctgcat agctatgcat gaaagcgaat ggagatcact ttgggagctt accgtgttca
      961 taccgtctgt tttcaacagt tttctccccg ggaagctaat ctgccataag cctggataac
     1021 agggcacggt gatactccgt aatagcgatc agcacctccg catactccct gtccccgata
     1081 cgcttgccgt cgataaagag ttttttctgt aacgccccca gtctcataac ttctttcagt
     1141 acccgatcat cagtcagtga gttaacgttc ttaccgagag ctgccgcccg taaaaaaccg
     1201 gaaacggtct tgccgcattc agcagccctt tttctgattt cctggtcttc ttctgccgtc
     1261 agacgaacag ggcgactgat agcccgcctg cgcgtattac ttccacttcg ttttgtcata
     1321 aaacatggct ccgtatctga catgggtgtc ggggcaaagc cctgaccagg gcaattgtaa
     1381 tagcgtgcat gtatgcgcgg tataacaatt gcacatcctg tcctgtcagc aagctcgaat
     1441 taccgacaaa ggcacggcag ttttttggca gttacagtgt tgtctgacag aaatgcccgt
     1501 caaaaagcca ggcggcagca aaacagtcga aaagtggtac gcgatataat cacataacgc
     1561 agatgacaga cagaacggag aaaacgaaaa gatgaacaag cagcagcaag ccgtactcaa
     1621 catggcggga ttcataaaaa gccagagcct gacgctgctc gaaaaactgg acgcactcga
     1681 tgctgacgag caggccacca tgtgtgagaa actacacgaa ctcgcagaag aactccagaa
     1741 cagcatacag acacgctttg aagtagaaaa tagcacagag agataacgct caggacagga
     1801 tgtcgaacgg cacaacagag cgtaaggaga aaatgtcaca taaggcgctc ttccgcttcc
     1861 tcgctcactg actcgctacg ctcggtcgtt cgactgcggc gagcggtatc agctcactca
     1921 aaggcggtaa tgcggttatc cacagaatca ggggataacg ccggaaagaa catgtgagca
     1981 aaaagccgaa ggcagggaca aaggccgcgc aggtggcgtt tttccatagg ctccgccccc
     2041 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     2101 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     2161 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     2221 cacgctgttg gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     2281 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     2341 cggtaagaca cgaattatcg ccactggcag cagccactga taaccgaatt agaggagtaa
     2401 gacttgaagt catgcgcctg ttaaggctaa actgaaagaa caagttttgg tgactgcgct
     2461 cctccaagcc agttacctcg gctcaaagag ttggtatctc tgagaacctt cgaaaaaccg
     2521 ccttgcaggg cggttttttc gtttacaggc agtagattac gcgcagaaaa aaaggatctc
     2581 aagaagaacc tttaattttt tttcacactt ggtatcctac tttaacccat aaaataaaca
     2641 cttacaccga cacgccctta aacaaaag
//
LOCUS       DAZPEB010000303         2653 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZPEB010000303 DAZPEB010000000
VERSION     DAZPEB010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.309"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..2344
                     /locus_tag="ACHURJ_004111"
     CDS             <1..2344
                     /locus_tag="ACHURJ_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR1282817.1"
                     /translation="VEKVGTYVTNTGLMATYGGQFTLTNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPRHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWDYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNRNISVGVSGQQWGIGYSLNYQYSRYTDENNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVIPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFNTPDSEPTTSVLQGTAQCH"
     gene            2355..>2653
                     /locus_tag="ACHURJ_004112"
     CDS             2355..>2653
                     /locus_tag="ACHURJ_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1282818.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKL"
BASE COUNT          740 a          640 c          621 g          652 t
ORIGIN      
        1 agttgaaaag gtgggcacct acgttaccaa tacaggctta atggctacat acggcggtca
       61 gtttacgctt acgaataaaa agaaggtttc acagaaaaaa attacattta ccgccaatgc
      121 agagcaactt ctgcagccac agtttacggt agaacaacta cgtgagctgg gtattaaggt
      181 ggatgaaatc ccggcgctgg ctgaaaaaga tgacgatagc gtgatcaact cgcttgaaca
      241 aatcattccc ggtacagctg ctgaatttga tttcaatcat cagcgactta atttgagcat
      301 tccccaaatt gcactgtacc gtgatgcaag aggttacgtc tccccttctc gttgggacga
      361 tggtatacca acgctgttta ccaactactc gtttacaggt tctgataacc gttaccgcca
      421 gggcaatcgt agccaacgac agtacctgaa tatgcaaaat ggtgctaatt ttggcccctg
      481 gcgattacgt aactattcta cgtggacacg caacgatcag gcgtcaagct ggaacactat
      541 cagtagttat ttacaacgtg atatcaaggc gttgaagtct cagttgcttc tgggagaaag
      601 cgccaccagc ggcagtattt tttccagcta cacctttact ggcgtgcaac tcgcttccga
      661 cgataatatg ttgccaaaca gccagcgcgg atttgcccca acggtacgcg gtatcgcaaa
      721 cagtagtgca atcgtgacta tcaggcaaaa tggttatgtg atctatcaaa gcaacgtgcc
      781 agcgggtgcc tttgaaatta acgatctcta cccctcttcc aacagcggcg atttagaagt
      841 cacgattgaa gaaagtgacg gtacacaacg tcgctttatc cagccttatt cttcattacc
      901 catgatgcag cgacctaggc atctaaaata tagcgcgacc gctggacgct atcgcgctga
      961 tgcaaacagt gatagcaagg aacccgaatt tgctgaagcc acggcaatat atggtttgaa
     1021 taatactttt acgctgtatg gcggcctgct cggttctgaa gattattatg cgctggggat
     1081 cggtatcggc ggcacacttg gcgcactggg cgccttgtcg atggatatca acagagctga
     1141 cacccaattc gataaccagc actcttttca tggctatcaa tggcgtacgc agtacatcaa
     1201 agatatcccg gaaaccaaca ccaatatcgc tgttagctac tatcgctata ccaacgatgg
     1261 ctattttagt tttgatgaag ccaatacccg caattgggac tataacagtc gccaaaaaag
     1321 tgaaattcaa ttcaacatca gccagacaat atttgatggg gtaagtctgt atgcctccgg
     1381 ttcgcagcaa gactattggg gcaataacga gaaaaacagg aatatctctg ttggggtttc
     1441 cggccagcaa tggggaattg gttacagcct gaattatcaa tacagccgct acactgatga
     1501 aaataatgac cgtgcactct ctttgaatct cagtattccg ttagaacgct ggttaccgcg
     1561 tagccgggtt tcctatcaga tgaccagcca gaaagatcgc ccaacccaac atgaaatgcg
     1621 tcttgatggc tcactgctgg atgatggtcg cctgagctat agcctggaac aaagtctgga
     1681 tgacgataac aaccataaca gtagcctgaa cgccagttac cgttcacctt atggaacctt
     1741 cagtgccgga tacagttacg gtaatgacag cagccaatac aattacggcg ttaccggcgg
     1801 cgtggttatc catcctcatg gcgtgacgct ctcgcaatat ctgggcaacg cttttgcgct
     1861 tatcgatgct aacggggctt ctggcgtgag gatacaaaac tatccgggga ttgctactga
     1921 tccctttggc tatgcagtga ttccttatct cacgacttat caggaaaacc gtctctcggt
     1981 agatactacg cagctgcccg ataacgtcga tcttgaacaa acaacacagt ttgtggtgcc
     2041 caacagaggt gcaatggtag cggcgcgttt caacgccaat atcggttatc gcgtacttgt
     2101 tacagtcagc gatcgcaacg gtaaaccgtt gccctttggc gctcttgcca gcaacgatga
     2161 tacggggcaa caaagtatcg tcgatgaggg cggcatacta tatctctctg ggatatcgag
     2221 taaatcacaa agctggactg tacgctgggg aaatcaggca gatcaacaat gtcagtttgc
     2281 ttttaataca ccggattcag aaccaacaac ctctgtatta caaggtacag cgcagtgcca
     2341 ttaaggataa aaaaatgaaa agagcgcctc ttataacagg acttttgttg atatccacat
     2401 cctgcgctta tgcctcctca ggagggtgtg gagccgacag cactagcggt gcgacaaatt
     2461 acagcagtgt ggttgatgat gttacggtga accagacaga taacgtgaca ggacgggagt
     2521 ttacctctgc aacgctaagt agcactaact ggcaatacgc ctgttcctgc tctgcgggta
     2581 aggcagttaa acttgtctat atggtcagcc ccgtacttac caccactgga catcagacag
     2641 gatattacaa act
//
LOCUS       DAZPEB010000304         2594 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZPEB010000304 DAZPEB010000000
VERSION     DAZPEB010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.310"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..566
                     /locus_tag="ACHURJ_004113"
     CDS             <1..566
                     /locus_tag="ACHURJ_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1282819.1"
                     /translation="RKIAGVGFDGSSDISISAKNVNAFALRQTGNTVNGDTSVGWNWD
                     SGAYNALIGGASALILHFNINAGSCPAVQFRVNYKNGGISYRSARDGYGFELGWSDFY
                     TTTRKPSAGDVGAYTQAECNSRFITGIRLGGLSSVQTWNGPGWSDRSGYVVTGSVNGN
                     RDELIDTTQARPIQYCINGRWYNAGSI"
     gene            569..1102
                     /locus_tag="ACHURJ_004114"
     CDS             569..1102
                     /locus_tag="ACHURJ_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR1282820.1"
                     /translation="MMHLKNITAGNPKTKEQYQLTKQFNIKWLYSDDGKNWYEEQKNF
                     QSDTLKMVYDHNGVIICIEKDVSAINPEGASVVELPDITANRRADISGKWMFKDGVVI
                     KRTYTEEEQRQQAENEKQSLLQLVRDKTQLWDSQLRLGIISDENKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(1131..1658)
                     /locus_tag="ACHURJ_004115"
     CDS             complement(1131..1658)
                     /locus_tag="ACHURJ_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR1282821.1"
                     /translation="MQHLKNIKSGNPKTKEQYQLTKNFDVIWLWSEDGKNWYEEVKNF
                     QPDTIKIVYDANNIIVAITKDASTLNPEGYSVVEVPDITANRRADDSGKWMFKDGAVV
                     KRIYTADEQQQQAESQKAALLSEAENVIQPLERAVRLNIATDEERARLESWERYSVLV
                     SRVDTANPEWPQKPE"
     gene            complement(1662..>2594)
                     /locus_tag="ACHURJ_004116"
     CDS             complement(1662..>2594)
                     /locus_tag="ACHURJ_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1282822.1"
                     /translation="DSILAWIRNTDWAKIGFKNDADSDTDSYMWFETGDNGNEYFKWR
                     SKQSTTTKDLMNLKWDALSVLVKALFSSEVKISTVNALRIFNSSFGAIFRRSEECLHI
                     IPTRENEGENGDIGPLRPFTLNLRTGRINMGHGLDVTGDITTNAWVYANRLAINSSTG
                     MWIHMRDQNVIFGRNAVSTDGAQALLRQDHADRKFMIGGLGNKQFGIYMINNSRTANG
                     TDGQAYMDNNGNWLCGAQIIPGNYGNFDSRYVRDVRLGTRVVRLMARGGRYEIAGHAL
                     TGLRIIGEVDGDDEAIFRPIQKYINGIWYNVAQV"
BASE COUNT          721 a          567 c          557 g          749 t
ORIGIN      
        1 cacgaaaaat tgctggagtt ggttttgatg gttctagcga tatttcaatt agtgccaaaa
       61 atgtcaatgc atttgcactc cgacaaacag gtaatactgt taatggtgat acatccgttg
      121 gatggaattg ggatagtggt gcatataacg ccctaattgg tggtgcatct gcattaattc
      181 ttcactttaa tataaatgct ggtagctgtc ctgccgtaca attccgtgtg aattataaaa
      241 atggtggcat ttcctacagg tcggctcgtg atggttatgg gtttgaatta ggttggtcag
      301 atttctatac cacgacacga aaaccttcag cgggagatgt tggtgcatat acacaggcag
      361 aatgtaactc aaggtttatt acaggtattc gccttggcgg tctgtcatct gttcagacat
      421 ggaatggtcc cggctggtct gacaggtcag gttatgtcgt tacgggttca gttaacggga
      481 accgtgatga attaattgat acaactcagg caaggccaat tcagtattgc attaatggaa
      541 ggtggtataa cgcggggagt atttaattat gatgcactta aaaaacatta ctgctggcaa
      601 ccctaaaaca aaagagcaat accagctaac aaagcaattt aacatcaaat ggctttattc
      661 agatgatgga aaaaactggt atgaggaaca aaagaatttc cagtcagaca ctttgaaaat
      721 ggtctatgac cataacggcg ttattatttg tattgaaaag gatgtttcag caattaatcc
      781 ggaaggcgca agcgtcgttg aattacctga tattacagca aatcgccggg ctgatatttc
      841 gggtaaatgg atgttcaaag atggcgtagt gataaagcga acttataccg aggaggaaca
      901 gaggcagcag gcagagaatg aaaagcaaag cctgttgcaa cttgtcaggg ataaaaccca
      961 gctatgggac tcacagctac ggctgggtat catttccgac gagaataaac aaaaattaac
     1021 cgagtggatg ctctatgcgc agaaggtcga atccacagac acctccagcc tgccagtaac
     1081 gtttccagaa caaccagaat gaaacaaggc ccgctatcgg gccttaattt ttattcaggc
     1141 ttttgtggcc attcaggatt tgccgtatcc acacggctga ccagaacact atagcgttcc
     1201 catgactcca gtcgtgcgcg ttcctcatcc gtcgcaatat tcagcctgac agcgcgttcc
     1261 agtggctgaa taacgttttc tgcttcggaa agtaacgcgg ccttttgtga ttcggcctgt
     1321 tgttgttgct cgtctgccgt ataaatccgt ttaaccacag ctccgtcctt aaacatccac
     1381 ttaccggaat catcagcacg acgattagct gtaatatcag gaacctcaac gacgctataa
     1441 ccttcagggt taagcgtgga ggcatctttg gtgatggcga caataatatt atttgcatcg
     1501 taaacaatct ttattgtgtc tggctgaaag tttttcactt cctcatacca gttttttccg
     1561 tcttcggacc ataaccagat aacatcaaaa ttctttgtta gctgatattg ttcttttgtt
     1621 tttggatttc cagacttaat attttttaaa tgctgcataa tttacacctg tgcgacgtta
     1681 taccatatgc cattgatgta tttttgtatc ggcctgaaga tagcttcatc atcgccatca
     1741 acttcaccaa taatccttaa tccggtaagt gcgtgtccgg ctatttcata acgccctcca
     1801 cgcgccatca atcgaacaac acgtgtgcca agtcggacat ctctcacata gcgtgagtca
     1861 aaattgccat aatttccggg aataatttgc gcaccgcaaa gccagttacc gttattgtcc
     1921 atgtacgcct gaccatcggt gccattggct gtccttgagt tattaatcat gtagatgcca
     1981 aattgcttat ttcccagacc gccaatcata aatttgcggt cggcatggtc ctgacggagc
     2041 aaagcctgag caccatcagt ggataccgca ttacgtccaa aaataacatt ctggtcacgc
     2101 atatgaatcc acatgcctgt actgctgtta attgcaaggc ggtttgcgta cacccatgcg
     2161 ttagttgtta tatctcctgt aacatccaga ccatgcccca tatttatgcg accagttctg
     2221 agattaagcg taaaggggcg tagcggccct atatcgccat tttccccctc attctctcgt
     2281 gtagggatga tatgcaggca ttcttcagaa cggcgaaaaa tggcaccaaa agatgaatta
     2341 aatatcctca gtgcattgac tgtcgatatt tttacttcac tgctgaaaag ggctttaaca
     2401 agaacagaca aagcatccca tttaagattc atcaggtctt ttgttgtggt gctttgtttg
     2461 cttctccatt tgaaatattc attgccgttg tcgcctgttt caaaccacat gtatgaatca
     2521 gtatcgctat cggcatcatt tttaaaacca atctttgccc agtcagtatt tcgaatccag
     2581 gcaaggattg agtc
//
LOCUS       DAZPEB010000305         2575 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZPEB010000305 DAZPEB010000000
VERSION     DAZPEB010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.311"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..323
                     /locus_tag="ACHURJ_004117"
     CDS             <1..323
                     /locus_tag="ACHURJ_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HFR1282823.1"
                     /translation="APFYNGKSDTRTVDLSDAVYRRLILMKAMSNITDCSVPDINRML
                     RFMFGKKRRAYVLNNGGLRMSYIFESALSLAELAIIQSSGALPSPPGVYVSVVLKESR
                     NEGQ"
     gene            1174..1695
                     /locus_tag="ACHURJ_004118"
     CDS             1174..1695
                     /locus_tag="ACHURJ_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1282824.1"
                     /translation="MDVYANSQRVFRFQNGVAIAFKNIQAGTARKFTLSSANNSTKNA
                     AFYLWGNPSRSVVAELGDDSGWHFFSQRNTDNSITFAVNGQVIPLNYGNFDARYKYRT
                     EGVQDVRYGHEMYYSPGSNTVSWRFRAPSGHGLSGIAISDTGRDSADNVNGVYYRPLQ
                     KLINGTWYNVASV"
     gene            1698..2243
                     /locus_tag="ACHURJ_004119"
     CDS             1698..2243
                     /locus_tag="ACHURJ_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR1282825.1"
                     /translation="MLHLKNITAGNPKTVEQYQLTKQYDVTWLFSKDGKNWYEELKNF
                     ASDTIKITYTGDGRVVWVGKDVTGIEPRNASVIEVPDITANRRITAPGYWFYRNDEFV
                     FDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISDEDREKLKAYRIYAKSLQAMDFS
                     VITDKTSYNAIEWPASPEISS"
     gene            2267..2557
                     /locus_tag="ACHURJ_004120"
     CDS             2267..2557
                     /locus_tag="ACHURJ_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR1282826.1"
                     /translation="MSVVISGALTDGAGIPMSGYHIILKSRVNTPEVVMHTVADVMTG
                     NDGEYCFHARTGKYGVYLKPGWHNEYNVGDIAVYEDSMRCTGNSGHYHLFFF"
BASE COUNT          678 a          566 c          695 g          636 t
ORIGIN      
        1 aggcaccgtt ttacaacgga aaatcggata cccggactgt tgacctgtct gatgctgtat
       61 accggcggct gatactgatg aaagccatgt cgaacattac tgactgttcc gttccggata
      121 ttaaccggat gctgagattt atgttcggaa aaaaacggcg agcttatgtt ctgaataatg
      181 gtgggctgag gatgagttac atctttgagt ccgcgctctc gttggcagaa ctggcgatta
      241 tccagtcgtc gggtgcactg ccgtccccgc cgggtgttta tgtttcagta gttttaaagg
      301 agtcccgtaa tgaaggccag tgataaaccc cgccagctgg cggtcccctt tgcgagtacc
      361 ggagataaaa accgtatccc ggacaaggcg acacagcaga ccagagagag cggtaatgct
      421 gcgtatgatt caggttttcc tccggtgacc atgacagcgg tctcagcggg aggtataccg
      481 ccacacggca aggattttaa cggtctgatg tacgatatta ccgcagcaat acggttcgcc
      541 caggctggcg gtttgtacac gtataatgcc ggttttgcgg gggccattgg tggatatgca
      601 aaaggagcca ttctcgccgg agtcgcaaca acagcggtct ggctgaatac cacagacgat
      661 aacctgaccg atcctgaagg ctccgacagt gcgggctggg taaatcttct tgaggatccg
      721 aaaaggatat tcctgcggca gaagaacaat ctgtcagacc ttcagaataa agggacagta
      781 cgggataatc ttcaggttta cagtaaagag cagtcagatc aacgctatgt tcatcgggaa
      841 ggcgataaaa taaccggaga gctgaaaatc cgtggtgtta atgcgctgag gattttcaac
      901 gaagcttttg gcctgatttt tcgtcgttcg gaagagtgcc tgcaccttat tcccaccagt
      961 gaaggtcagg gggaaaatgg cgatattggt cccctgcgcc cgttcaccat taatttgcgg
     1021 acgggtgaaa tatccatgtc gcataaagtg tctgttggcg gtggttcgca ggtcaatggt
     1081 gcgctgggta tcggcgttca gaacgccctg ggggggaatt caattgttct tggtgataat
     1141 gacaccggat ttaaacagaa tggagacggt attctggatg tttatgctaa tagtcagcgt
     1201 gtattccgct ttcagaatgg agtggctatt gcttttaaga atattcaggc cggaactgcc
     1261 agaaaattca cgttatccag cgccaacaac tccacgaaaa atgcagcgtt ttatttgtgg
     1321 ggtaatccat ccaggtctgt tgttgcagag cttggcgatg attcaggctg gcattttttc
     1381 agccagagga atacagataa cagcatcacg ttcgccgtaa acggacaggt aattccgtta
     1441 aattacggaa acttcgatgc ccgctataaa tatcgaacag agggggtaca ggatgtacgg
     1501 tatggccatg aaatgtatta cagccccggc agtaacaccg tttcgtggag atttcgcgca
     1561 ccttcgggac acgggctgtc agggatagcg atatcggata ccggccgtga ctcagcggat
     1621 aacgtcaacg gcgtgtatta ccgcccgctt cagaaactga ttaatggcac ctggtataac
     1681 gtggcgagtg tttaacaatg ttgcatttaa aaaatattac tgcgggcaat ccgaaaaccg
     1741 tggaacaata tcagctgaca aaacaatatg atgtcacctg gcttttttcg aaagacggaa
     1801 aaaactggta tgaagagctg aagaatttcg ccagcgacac aattaaaata acttacaccg
     1861 gagacggtcg cgtggtgtgg gtcggtaagg atgtgactgg cattgaaccc cgtaacgcca
     1921 gtgttattga agttcctgat attaccgcca accgccgcat taccgcgccg ggttactggt
     1981 tttaccgcaa tgatgaattt gtctttgact acagactcaa agcggaagat gagcgtgatg
     2041 cccttctggc tcaggtcagt gcccggacag gggaatggga agaagacctg ctgctggggc
     2101 taatcagcga cgaagaccgg gaaaaactga aagcgtaccg catttacgcg aaatcgctgc
     2161 aggcgatgga tttcagtgtc atcactgata aaacctcata caacgccatt gaatggcccg
     2221 cctctccgga aatttcttcc tgatttaatt tatcgcgaga aaaactatgt ctgtagtgat
     2281 atcaggtgcg ctgactgatg gcgcaggtat ccccatgtcc ggataccata ttattctgaa
     2341 atcccgggta aacaccccgg aagtggtgat gcacactgtt gctgatgtga tgacaggaaa
     2401 cgatggtgaa tactgtttcc atgcgcggac tggaaaatat ggtgtgtatc tgaaaccggg
     2461 ctggcacaac gagtacaacg ttggcgacat tgctgtatat gaggactcaa tgaggtgtac
     2521 tggcaatagc ggacactacc atttgttctt tttttaagca gctatctgat gatat
//
LOCUS       DAZPEB010000306         2533 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZPEB010000306 DAZPEB010000000
VERSION     DAZPEB010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.312"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..185
                     /locus_tag="ACHURJ_004121"
     CDS             <1..185
                     /locus_tag="ACHURJ_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282827.1"
                     /translation="DDGGRFKKTLTVRAREQVAKIVSKNDPDTKKVWCKYGKIPRQGD
                     GVNLFLLVKLMLRIIL"
     gene            complement(747..1112)
                     /locus_tag="ACHURJ_004122"
                     /pseudo
     CDS             complement(747..1112)
                     /locus_tag="ACHURJ_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(1228..2322)
                     /gene="mnmH"
                     /locus_tag="ACHURJ_004123"
     CDS             complement(1228..2322)
                     /gene="mnmH"
                     /locus_tag="ACHURJ_004123"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFR1282828.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRIDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLHAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAR"
     gene            complement(2391..>2533)
                     /locus_tag="ACHURJ_004124"
     CDS             complement(2391..>2533)
                     /locus_tag="ACHURJ_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="activator of the allDC-ylbA operon involved in
                     allantoin utilization; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFR1282829.1"
                     /translation="MRPPSPLSLAWRKFGSGKAVEDIVTLFTQRRPEISGFLEIFGNP
                     RS"
BASE COUNT          658 a          631 c          610 g          634 t
ORIGIN      
        1 atgatgatgg gggtaggttt aagaaaacat tgacagtgag ggccagggaa caagtggcga
       61 aaatcgtatc aaagaatgat ccagatacaa aaaaagtgtg gtgtaaatat ggtaagatac
      121 caaggcaagg ggatggtgta aacctttttt tgttggtgaa attaatgtta cgcattattt
      181 tataacaaat attggagctg gattgcctga tgcttgtgca gagtaattgc ttgaattaag
      241 agtctatccc atatcgaagt cgtcaacttc gtagtgagga aaagtaaaat tcctgactga
      301 gaaaagacat gtcggctatt gtgtaaagcc atatagctca gacgatgaat atctactcgt
      361 attcagttgt ttattgaggg tgagttccga ccctgaaaca acaaataaaa tgaacagtca
      421 gagagtttac atagaattgc actggtcttt tacgatatct gacattgtgt aatacatatt
      481 cagccatgca ttaattaaaa tgttacgtgt ttaatgtgag tccctatctg aaaataaata
      541 atccttccgg attaaaataa attcttgccg ggaaagaaag aggaaataaa ccattagcgg
      601 aaaaccagcg gcacgccagg gtgacatgcc ccagtacggc ggcccgatag tccagggttc
      661 agcgggggta ctgatagtcg cgccgactgc atcgagaact ccatacaact ttttacggaa
      721 cgtcacctga taaaatttgt tcagtatcgt cttttgagat tctcaaggtt atggagtaac
      781 cagacagaac ggacaccact gtcggagata aaacccccct gtttacgcag ctcgttgctg
      841 gcccggtgct gaccatgtgt cgggaaagcg acggcgtaat caacaacagc ctgttcagtt
      901 gcctcatcgg tacggttctt aaggttagga gcgcggcgac tacgattaat ctgcgcatcc
      961 acaccgcctt cagcgaccag ttcgcggtaa cgataaaacg tatcacgcga aacgcccatg
     1021 attttacagg cttttgatac gttgctgagt tcttcagcca gattgagcaa accggctttg
     1081 tgtttgatga cgggattggc agtatgaagc atgagagtta cctcttgttt tggataagga
     1141 ttcgacactc atatcaaaac cggtaactct caacctttca aggccatgtg tcagatcaag
     1201 tcgcgactaa tacaaatacg tccctcatta ccgcgcctta acccattccg ccacttccgc
     1261 ccactcaccg cgaaagacaa ctttttccgc ttttttctca agctgatagc gatacattgg
     1321 gtcgtaatat tctttaagta acggcaccag ccaggccaga tgaccgtcgg tgctgccggt
     1381 ggtgagttgc gttgtcagcg ccgtatccag ctgggcagcg agttcgttat agcgctgtaa
     1441 ccccagccga cgcttaatag ccgaaagtcc gtgatgcagg tattcgcaat attcctgcca
     1501 gccctgttcg tcgccgtacg cgtgggtaaa atcatgatgc atacgcaaga aatactcttc
     1561 gttcaggcgt tcaagacgga tctcaaacgg atcttctacc accgcaatcg ccgcctgagt
     1621 cattcgctcg cgcagacatt ccggcaggtg attcgaaccg atcatccggc tttcatcttc
     1681 cagcacccac aggcgcaaat cctgacgggc gtcggttttt agcatttcgg cagccagcag
     1741 attttcaaaa ctcgcctggc taagttgtgg ttgtaacgtg cgaccaaacg ccgaaccgcg
     1801 atgacgcgcc agcccttcca gatcgacacc gttcggctgt tgctgcacta acagcgtttt
     1861 accgctgccg gtacaaccgc caatcagcac tatcggtttt tgtgccagtt caatagtcgc
     1921 ctgaatcgcg gtctggcgca gtgccttata accgccttcc accagcggat aatcaatccc
     1981 cgctgcatgc aaccagcttt gcacaatatg tgagcgttga ccgccacggg cacagcagag
     2041 aataccttgc ggattttgca ggcacgctgc ccgccaggcg tctatgcgct gctgacgaat
     2101 ttcacccgcc accagtttat gccccagcgc cagcgccgcg tctgaccctt gctgtttata
     2161 gcaggtgcca acggcggcgc gttcatcgtt attcattaac ggcagattga tagcggcggg
     2221 cattgcgcct tgctcaaact cgataggggc gcgaacatca ataatgggcg tatcagcaat
     2281 cagcagggca cgatagtcct gttccgtgtg tctctcttgc atagttaaaa gtgaacctca
     2341 aatcagcttg cgcgctattt tacgcgccaa cgcgcaagga aacttgattt ttaactgcgt
     2401 gggttgccga aaatttctaa aaatccgctg atttccggcc tgcgctgggt aaacaaggtc
     2461 acaatatctt ctaccgcttt gccgctgcca aatttgcgcc atgccagact caatggcgaa
     2521 ggagggcgca tcg
//
LOCUS       DAZPEB010000307         2468 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZPEB010000307 DAZPEB010000000
VERSION     DAZPEB010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.313"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            128..1264
                     /gene="potA"
                     /locus_tag="ACHURJ_004125"
     CDS             128..1264
                     /gene="potA"
                     /locus_tag="ACHURJ_004125"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFR1282830.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            1248..2111
                     /gene="potB"
                     /locus_tag="ACHURJ_004126"
     CDS             1248..2111
                     /gene="potB"
                     /locus_tag="ACHURJ_004126"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFR1282831.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVSELDD"
     gene            complement(2343..2438)
                     /locus_tag="ACHURJ_004127"
     CDS             complement(2343..2438)
                     /locus_tag="ACHURJ_004127"
                     /inference="COORDINATES: protein motif:HMM:NF014467.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR1282832.1"
                     /translation="MAKFSVPLQVLLNRVDTSTAPDIEWPTSPAE"
BASE COUNT          592 a          606 c          621 g          649 t
ORIGIN      
        1 ctaagccagt aattcacaac aaagattggc gattcaagcg gtttgccgta gaattaccgc
       61 cctcattaag agtcaccaag gtggttaacc acaaaccccg catcggtaag ccatccgttg
      121 cgtttacatg ggacagagta aaaaattgaa taaacaaccg agttcgcttt caccgctggt
      181 gcaattggcg ggaattcgca aatgctttga tggtaaagag gtcattcccc agctggatct
      241 gactatcaac aatggcgagt tcctcacgct gcttggccct tctggctgcg gtaaaacaac
      301 cgttcttcgc ctgattgcag gtctggaaac tgttgattcc ggacgcatca tgctggataa
      361 cgaggacatc acccacgttc cggcggaaaa ccgctatgtg aacactgttt tccaaagcta
      421 cgcacttttc ccccacatga ccgtgttcga aaatgtggcc tttgggttgc gcatgcaaaa
      481 aacccccgct gctgaaatta cgccccgcgt gatggaagcc ctgcggatgg tgcaactgga
      541 aaccttcgct caacgtaaac cgcatcagct ctctggtggt caacagcaac gcgtcgccat
      601 tgctcgcgcg gtggttaaca agcctcgtct gttgttgctg gatgagtcgc tctcagcgct
      661 ggattacaaa ctgcgtaagc aaatgcagaa cgagctgaaa gcgttacagc gtaagcttgg
      721 cattacattc gtcttcgtga ctcacgacca ggaagaagca ctcaccatgt cagacaggat
      781 tgtggtgatg cgcgagggtc gcattgagca agacggcacg ccgcgtgaaa tctacgaaga
      841 gccgaaaaac ctgtttgttg ccggcttcat tggcgaaatt aatatgttta acgccacggt
      901 gatcgagcga ctcgacgagc agcgcgtacg cgccaacgtt gaaggccgcg aatgtaatat
      961 ctacgttaac ttcgccgttg aaccggggca aaaactgcat gttctgctgc gcccggaaga
     1021 cttacgtgtt gaagagatta acgacgacaa ccacgctgaa gggctgattg gttacgttcg
     1081 tgagcgtaac tacaaaggca tgacgctgga gtcggttgtt gaactggaaa atggcaagat
     1141 ggtgatggtc agcgaattct tcaatgaaga cgatcctgac tttgaccact ctctcgacca
     1201 aaaaatggcc attaattggg tagaaagctg ggaggtcgta ctggctgatg aagaacacaa
     1261 gtaagttcca gaatgtagtg attgtcacta ttgtcggttg gcttgtgttg tttgtctttc
     1321 tgcccaacct gatgatcatt ggcaccagct ttttgacccg cgacgacgcc agtttcgtca
     1381 aaatggtctt tacgctggat aactacacgc gtctgctcga tccgctctat tttgaagtgc
     1441 tattgcactc gctgaatatg gcgctgatcg ccaccctcgc ctgcctggtg ctgggctatc
     1501 catttgcctg gtttctggcg aagctgccac acaaggtgcg tccgctgctg ctgtttctgc
     1561 tgattgttcc gttctggacc aactcattga ttcgtatcta cgggctgaaa attttcctca
     1621 gcaccaaagg ctatctcaac gagtttttgc tctggctggg cgttatcgac acaccaatcc
     1681 gtatcatgtt cacgcccagt gcggtgatta tcggtctggt ttacattctg ctgccgttta
     1741 tggtgatgcc gctgtactcc agtatcgaaa aactggataa gccgttgctt gaggcggcgc
     1801 gcgatctcgg tgccagcaag ttacagactt ttatccgcat cattattcca ctgacgatgc
     1861 cggggattat tgccggatgt ctgctggtga tgctgccagc gatgggcctg ttctatgtgt
     1921 ccgacctgat gggcggtgcg aaaaacctgc tgatcggtaa cgtcatcaag gtacagttcc
     1981 tcaatattcg tgactggccg tttggtgcag ccaccagcat tacgctgact atcgtaatgg
     2041 gcctgatgtt gctggtttac tggcgcgctt ctcgtttgct gaataagaag gtgagcgaat
     2101 tagatgatta atcatttata ttcaatgcat taagtttgtt tttgagtggt tttgccgttt
     2161 aaggtaacgg cgttatagat cctatcaaaa tcaatcaatt ccattctggt tttggcgaaa
     2221 gatttttcga gagcttcggt ctttaactaa aactgattgc gtataacccc aatacccctc
     2281 cccgactatc tgaattgtcg gggaggcact tcacgcacaa aatatcatcc ctgcgccgtt
     2341 gattactctg caggtgaagt cggccactca atatcaggtg cagttgatgt atcaacacgg
     2401 ttcagcaaca cctgaagtgg cacactgaat ttggccacct gaacagaggt gatatgctca
     2461 cctcagaa
//
LOCUS       DAZPEB010000308         2452 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZPEB010000308 DAZPEB010000000
VERSION     DAZPEB010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.314"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..985)
                     /locus_tag="ACHURJ_004128"
     CDS             complement(<1..985)
                     /locus_tag="ACHURJ_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFR1282833.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVIIYKVSNAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDEDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCNFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQASCWNDVAFIPATNEKQYQVKYQHAKKNGDTFNAFIYFI"
     gene            complement(1011..1703)
                     /gene="rfaY"
                     /locus_tag="ACHURJ_004129"
     CDS             complement(1011..1703)
                     /gene="rfaY"
                     /locus_tag="ACHURJ_004129"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HFR1282834.1"
                     /translation="MITSIRYRGFSFYYKDNDNKYKEIFDEILAYNFKTVKVLCNIDD
                     TKVSLIDTKYGRYVFKVFAPKTKRNERFLKSFVKGDYYQNLIVETDRVRSAGLTFPND
                     FYFLAERKIFNYASVFIMLIEYVEGVELNDMPIIPENVKAEIKASMEKLHALNMLSGD
                     PHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIANEIKDYGYYSVIYRTK
                     LRKFIKKLKGKA"
     gene            complement(1713..>2452)
                     /locus_tag="ACHURJ_004130"
     CDS             complement(1713..>2452)
                     /locus_tag="ACHURJ_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFR1282835.1"
                     /translation="DPKYFADLPTSQFWSYATYFRVLSFEYLSESISTLLYLDADVVC
                     KGSLKPLTEIIFKDEFAAVIPDNDSTQAACAKRLNIPEMNGRYFNAGVIYVNLKKWHE
                     ANLTPYLLKLLRGETKYGSLKYLDQDALNIAFNMNNIYLAKDFDTIYTLKNELYDRSH
                     RKYQQTITDKTVLIHYTGITKPWHSWAGYPSASYFNIAREQSPWKKYPLKEARTVAEM
                     QKQYKHLFAHGEYIKGITSLIKYKLKK"
BASE COUNT          759 a          487 c          425 g          781 t
ORIGIN      
        1 taatgaagta aataaaagcg ttaaacgtat caccattttt ctttgcatgt tgatatttca
       61 cttgatactg cttttcattc gtggctggaa taaacgccac atcattccag caagaagcct
      121 gataagcctc attaaaatac ttctggcagg gataattcgt ccagtcgttc cagggcttcg
      181 ttacgccaac ataatgaatc agcttagtat catcggtaat ggtcttttta taatcttcat
      241 cgcttttatt ttttaactca tagtcaatgc cataaaagca atcataatcg ccgctaataa
      301 aattacaatg accaaagaac agaatattta aaatatcctg atcaaggcat ttatacttcg
      361 tgtgctgcgg gtcacttgcc agcatagcaa tggctttatt cataaaatca tttttcgccc
      421 atgcttcaag ctgcagatat actacaccgg cgttgaaata attccctgga agtccctcta
      481 tagccagacg cttcgctggt ttttcctgca taaaaggcac atctttaata acagctgcga
      541 acttatcctc atcaaaatta atatcaagca atccagctaa tgagccttta cagatgacat
      601 cagcatcgat atataacaga ttttcaatag tttcactcaa atactggaac gcaactaaac
      661 ggaagaatgt tgcataagac cactgtttca ggctggggaa gatatcaata aaggcattgg
      721 agactttata aattattact tcaacgttag catttttgct ggtaaaagca ttaacacgtt
      781 gtagataatc ttcatcaatg tagtcggtaa agagatgaaa cttaaccgca aggtccgggt
      841 tatgcataac aactgattgc atagatactg ccgcaccaaa taaaaagtta cggtcaatac
      901 cgtaagcgat atttaacaca cgatcggtgt tagcaatttc cccagagaca gcgacttcag
      961 taatactctc tgctaataaa tccatgttgg ttccgttttg actgtgtggt ttacgctttg
     1021 ccttttaatt ttttaataaa tttgcgtagt ttggttctgt agattactga atagtagcca
     1081 taatctttta tttcgttggc aatacccagg tgacgttcca tggccagacg atcacgagct
     1141 ttacgctctg ccgtacagct tttaccggat aggtcaatta tcctgacacc atctttacta
     1201 acgataaagt taccccggtg tgggtcgccc gatagcatat ttaaggcatg caatttttcc
     1261 atcgaagcct taatttctgc tttaacattt tctggaataa tcggcatatc attgagctct
     1321 acgccctcga catactcgat aagcatgata aaaacactgg catagttgaa aattttacgc
     1381 tcagccagaa aataaaaatc attaggaaaa gtaagacctg cacttctgac acgatcggtt
     1441 tcaacgatca aattctgata gtagtcacct tttacaaaag attttaaaaa acgttcattc
     1501 cttttcgttt tcggagcaaa gactttaaaa acatagcgac catatttcgt atcaatcaac
     1561 gatactttgg tatcatcaat attacataat acttttactg ttttaaaatt ataggccagt
     1621 atttcatcaa agatttcttt atatttatta tcattatctt tgtaataaaa tgagaagccg
     1681 cgataacgta tacttgtaat cataagagta atttatttct taagcttgta cttaattaat
     1741 gaagttatgc cttttatata ctcaccatgg gcaaacagat gcttatattg tttctgcatt
     1801 tctgcaacag tccgcgcctc tttaagagga tatttcttcc agggagattg ttcacgcgcg
     1861 atattaaagt atgatgcaga cggatatcca gcccagctat gccatggttt agttatccct
     1921 gtatagtgaa tcaacactgt tttatcggta atggtttgct gatactttcg atgactacga
     1981 tcataaagtt cgtttttcag ggtataaata gtatcaaaat ccttcgcaag gtagatatta
     2041 ttcatattaa atgcgatatt caacgcatcc tgatctaaat atttaagaga gccatattta
     2101 gtttcccctc gtaaaagttt gagtaaatac ggtgtcaaat ttgcttcatg ccatttttta
     2161 agattgacat agataacgcc tgcattgaaa taacgtccat tcatttcggg aatgttgagg
     2221 cgttttgcac atgccgcctg agtactatca ttgtcaggaa tgaccgcagc aaactcatct
     2281 ttaaatataa tttctgtgag aggtttcagg cttcctttac aaacaacatc ggcatccaga
     2341 tacagcagtg tggaaatact ttcactcaga tattcaaaag acaataccct gaagtatgtc
     2401 gcgtatgacc aaaactgtga cgtcggcaga tcagcaaagt atttggggtc aa
//
LOCUS       DAZPEB010000309         2451 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZPEB010000309 DAZPEB010000000
VERSION     DAZPEB010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.315"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..566
                     /locus_tag="ACHURJ_004131"
     CDS             <1..566
                     /locus_tag="ACHURJ_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1282836.1"
                     /translation="RKIAGVGFDGSSDISISAKNVNAFALRQTGNTVNGDTSVGWNWD
                     SGAYNALIGGASALILHFNINAGSCPAVQFRVNYKNGGISYRSARDGYGVELGWSDFY
                     TTTRKPSAGDVGAYTRAECNSRFITGIRLGGLSSVQTWNGPGWSDRSGYVVTGSVNGN
                     RDELIDTTQARPIQYCINGTWYNAGSI"
     gene            569..1102
                     /locus_tag="ACHURJ_004132"
     CDS             569..1102
                     /locus_tag="ACHURJ_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR1282837.1"
                     /translation="MMHLKNITAGNPKTKEQYQLTKQFNIKWLYSDDGKNWYEEQKNF
                     QPDTLKMVYYHNGVIICIEKDVSAINPEGASVVELPDITANRRADISGKWLFKDGVVI
                     KRTYTEEEQRQQAENEKQSLLQLVRDKTQLWDSQLRLGIISDENKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(1131..1694)
                     /locus_tag="ACHURJ_004133"
     CDS             complement(1131..1694)
                     /locus_tag="ACHURJ_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000930007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR1282838.1"
                     /translation="MAHGITSHRCKLCSILKNIKSGNPKTKEQYQLTKNFDVIWLWSE
                     DGKNWYEEVNNFQDDTIKIVYDENNIIVAITKDAPTLNPEGFSVVEIPDITANRRADD
                     SGKWMFKDGTVVKRIYTADEQQQQAESQKVALLSEAESVIQPLERAVRLNMAMDEERT
                     RLESWERYSVLVSRVDTANPEWPQKPE"
     gene            complement(1663..>2451)
                     /locus_tag="ACHURJ_004134"
     CDS             complement(1663..>2451)
                     /locus_tag="ACHURJ_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1282839.1"
                     /translation="DSILAWIRNTDWAKIGFKNDADGDTDSYMWFETGDNGNEYFKWR
                     SRQSTTTKDLMTLKWDALNILVNAVINGSLGVGSTNALGGSSIVLGDNDTGFKQNGDG
                     ILDVYANSQRVFRFQNGVAIAFKNIQAGDGKKFMLSSSNNSTKNATFNLWGASTRPVV
                     AELGNEAGWHFYSQRNTDNSVIFSVNGQIQPSNWGNFDSRYVKDVRLGTRVVQLMARG
                     GRYEKAGHAITGLRIIGEVDGDDEAIFRPIQKYINGTWYNVAQV"
BASE COUNT          681 a          537 c          520 g          713 t
ORIGIN      
        1 cacgaaaaat tgctggagtt ggttttgatg gttctagcga tatctcaatt agtgccaaaa
       61 atgtcaatgc atttgcactc cgacaaacag gtaatactgt taatggtgat acatccgttg
      121 gatggaattg ggatagtggt gcatataacg ccctgattgg tggtgcatct gcattaattc
      181 ttcactttaa tataaatgct ggtagctgtc ctgccgtaca attccgtgtg aattataaaa
      241 atggtggcat ttcctacagg tcggctcgtg atggttatgg ggttgaatta ggttggtccg
      301 atttctatac cacgacacga aaaccttcag cgggagatgt tggtgcatat acgcgggcag
      361 aatgtaactc aaggtttatt acaggtattc gccttggcgg tctgtcatct gttcagacat
      421 ggaatggtcc cggctggtct gacaggtcag gttatgtcgt tacgggttca gttaacggaa
      481 accgtgatga attaattgat acaacacagg caaggccaat tcagtattgc attaatggga
      541 cgtggtataa cgcggggagt atttaacgat gatgcactta aaaaacatta ctgctggcaa
      601 ccctaaaaca aaagagcaat accagctaac aaagcaattt aacatcaaat ggctttattc
      661 agatgatgga aaaaactggt atgaggaaca aaagaatttc cagccagaca ctttgaaaat
      721 ggtctattac cataacggcg ttattatttg tattgaaaag gatgtttcag caattaatcc
      781 ggaaggcgca agcgtcgttg aattacctga tattacagca aatcgccggg ctgatatttc
      841 ggggaaatgg ttgttcaaag atggcgtagt gataaagcga acttataccg aggaagagca
      901 gaggcaacaa gcggaaaatg aaaagcaaag cctgttgcaa cttgtcaggg ataaaaccca
      961 gctatgggac tcacagctac ggctgggcat catttccgac gagaataaac aaaaattaac
     1021 cgagtggatg ctctatgcgc agaaagtcga atctacagac acctccagcc tgccagtaac
     1081 gtttcccgaa caaccagaat gagagaaggc ccgatattgg gccttaattt ttactcaggc
     1141 ttttgtggcc attcgggatt tgctgtatcc acacggctga ccagaacgct gtagcgttcc
     1201 catgactcca gtcgtgtgcg ttcctcatcc atcgccatat tcagcctgac agcgcgttcc
     1261 agtggctgaa taacgctttc agcttcagaa agcaacgcga ccttttgtga ttcggcctgt
     1321 tgttgctgtt cgtctgctgt ataaatccgc ttaaccacag ttccgtcctt aaacatccac
     1381 ttccctgaat catcggcacg acgattggct gttatatctg gaatctcaac gacactaaag
     1441 ccttcgggat taagcgttgg ggcatctttg gttatggcaa caataatatt attttcgtcg
     1501 tatacaatct ttatggtgtc gtcctgaaag ttatttactt cctcatacca gtttttaccg
     1561 tcttcggacc ataaccagat aacatcaaaa ttctttgtta gctgatattg ttcttttgtt
     1621 tttggatttc cggacttaat attttttaat atgctgcata atttacacct gtgcgacgtt
     1681 ataccatgtg ccattgatgt atttttgtat tggtctgaag atggcttcat catcgccatc
     1741 tacttcacca atgattctta atccggtaat tgcgtgtccg gctttttcat aacgaccacc
     1801 acgcgccatc aattgaacaa cacgcgtacc caggcgaaca tcttttacat agcgtgaatc
     1861 aaaattcccc cagttgctgg gctgtatctg accgttaaca gaaaatatta ccgagttatc
     1921 tgtatttcgc tggctataga aatgccatcc tgcctcattg cctaactctg caactactgg
     1981 acgggttgaa gcaccccata aattaaacgt tgcattcttt gtggagttgt tggagctgga
     2041 tagcatgaat tttttaccat ccccggcctg aatattttta aaagcaatag ccactccatt
     2101 ctgaaagcgg aatacacgct gactattagc ataaacatcc agaataccgt ctccattctg
     2161 tttaaatccg gtgtcattat caccaagaac aatagagcta ccacctaacg cattcgtcga
     2221 accaactcca agactgccat taatgacagc attaacaaga atatttagtg catcccattt
     2281 cagcgtcatc aggtcttttg ttgtggtgct ctggcggctt ctccatttga aatattcatt
     2341 gccgttgtcg cctgtttcaa accacatgta tgaatcagtg tcaccatcgg catcattttt
     2401 aaatccaatc ttcgcccagt cagtatttcg aatccaggca aggattgagt c
//
LOCUS       DAZPEB010000310         2445 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZPEB010000310 DAZPEB010000000
VERSION     DAZPEB010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.316"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..754)
                     /locus_tag="ACHURJ_004135"
     CDS             complement(<1..754)
                     /locus_tag="ACHURJ_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282840.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLALLPHAHQQQNPPQEETVVSIAVDPESPA"
     gene            complement(756..1034)
                     /locus_tag="ACHURJ_004136"
     CDS             complement(756..1034)
                     /locus_tag="ACHURJ_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282841.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDWYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
     gene            complement(1202..1357)
                     /gene="hokD"
                     /locus_tag="ACHURJ_004137"
     CDS             complement(1202..1357)
                     /gene="hokD"
                     /locus_tag="ACHURJ_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR1282842.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(2009..2425)
                     /locus_tag="ACHURJ_004138"
     CDS             complement(2009..2425)
                     /locus_tag="ACHURJ_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282843.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
BASE COUNT          579 a          639 c          573 g          654 t
ORIGIN      
        1 gagccggaga ttccggatca acggcaatgc tgaccaccgt ctcttcctgt ggtggattct
       61 gttgctggtg ggcgtgaggc agcagcgcaa gattttttgt gcgctgcttc agtatgctgg
      121 tggcggtctg ctctcccggc acgatgtcgc tttcacggta cattgagcgg attttttccg
      181 cacgcaaccc cagcgaacga cgtaatactg cttccggtag cgcgtccgcc acctgattgc
      241 ggaccgccca ccaggataat tcagccagag ataattcacg ctcctgcgta ccgcttattg
      301 cgtgaccgat gacgtcaatc atccatgctg acaggttttg atgagcaagt tgctcgagtg
      361 attcggatgt ctggtcacgc aactggttgt cgcagtgcca gcacaacacc attgcgccgg
      421 taccataacg gtgaatgacg gtttcgctgt gatgataatc gccgtgtggc cactggcagg
      481 atttaatatg gcgcaacagc cagtcagaca atgcaccagc accaccagca gcacgaatca
      541 cccgtgcgtt actgaaaaac ggcagcaatg ttttgtcttc cactagcggc tggcgaacgg
      601 caggaacgac cccggacggc agattacgca tgcttttcgg ttccggctcc accagtaccc
      661 gggtattgtg gaataccggc atggattcac ggcctggctt aaggaccacc agcccgagtt
      721 ccggaaccag aacaggtcga agtaataccc gcacattacc tccagatccg ttgctggaat
      781 gtgcaggacg gacgcggtgg ccgttcggaa taagggagcc tgacgaagat tatccagtga
      841 cgataatcga gactgagggc tttcttaatc tcgtatccgt gtctgcggta gcgctgaatc
      901 agccattcag cctgttcttc ggtgcagggg tcgtgctgat accagtcaga tttgaatgca
      961 tgagaacgcc gcccgtgcct gctggcaggc gcggcagagt tatccgaatt gtaaaatttg
     1021 gtatcgtgcg ccatctgttt tctctgctgg cgcagcaggt gccagttgtt caggctgacg
     1081 gatggattgt aaaccagaac gaccagaaaa aacaaaaccc gccgaagcgg gttaagtgcg
     1141 ggtgcgttga ggatgcctga cacatcagcg gtggcgaggg atttctcccc cgccgggtct
     1201 cttactcctc aggttcgtaa gctgtgaaga cagcgacctc cgtctggccg gttcggattc
     1261 gtacctcgca gaggtctttc ctcgttacca gtgccgtcac tatgacggtt aaacagatga
     1321 cgataagggc gattaacatc gccttttgct gcttcatagc ctgcttctcc ttgcctttcg
     1381 gcacgtaaga ggctaaccta catgtgttca gcatggattg agcctcagat taatgttaag
     1441 cgtcttgcag gacgcgtagt gttaactggg gcttttctct atctgccttt tggtgttcat
     1501 gcctgagaca gatagcctca agcacccgca gtcattctac ttaactaaga tttccctgca
     1561 aaccgttttt gtccggcaca gtaaatatcc aactaaacca atagcgttcg ctgtatttac
     1621 cgccagtatt caatgcacat gaccgccatg aacaccccta aaaaaagggc atttatatgt
     1681 ccaaacatta atatcaaaac atcaattttt tccatatacc ttgctgtgaa gatgatgggc
     1741 atacatgatg cgaacaacca gaacgcaaca aacaaaaact gcaatgcgtt tttcattatt
     1801 ccccctacaa tcaatgtgca ataacattta aacacacctc aatttggccg gacatataaa
     1861 tatctaaacc agaaaaaatc acttacatag cgttacaaac tctttagtct aaatactcat
     1921 cgtaaaacat tccccatact tatcagtccg ttccgcgcca ggtagcttat tgccttatct
     1981 ggcaacctat aatcaggttt ccgctttttc agttggctgg tcgtttaacc gacatagtta
     2041 acccattaat ctggttgccg gatgctggtg gattttcgcg ttttagttgt tcataaaagt
     2101 gcacagcttt aaccagttct tctgatgtaa ccgggactgg tggggcagtg aataaggcct
     2161 gaatttcata gttcggcctg tcgttacaat cctctttttt cggtacatat ttccagtcac
     2221 caacccacaa ctccccctga gagtccataa cacctttttt cacgtagcga tatcgccacg
     2281 ctatcggctc tgcttccagc gatgcccgtg caattttgaa taactcgccc tctactcgcg
     2341 ccatccctga attggggtgg catttcgtaa tcgctatttt taatttggct tcttcgatta
     2401 attgttcttt tgttaattca gtcattttca ttaccgccct ttcag
//
LOCUS       DAZPEB010000311         2414 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZPEB010000311 DAZPEB010000000
VERSION     DAZPEB010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.317"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..450
                     /locus_tag="ACHURJ_004139"
     CDS             <1..450
                     /locus_tag="ACHURJ_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HFR1282844.1"
                     /translation="AVLIGLIALLAWPLSEATGRMFGLGITSPTANILQFLVAGDVKY
                     INWGVFLVLGIFVGSFIAAKASREFRVRAADAQTTLRSGLGGVLMGFGASIAGGCSIG
                     NGLVMTAMMTWQGWIGLVFMILGVWTASWLVYVRPQRKARLATAAAN"
     gene            464..691
                     /gene="tsuB"
                     /locus_tag="ACHURJ_004140"
     CDS             464..691
                     /gene="tsuB"
                     /locus_tag="ACHURJ_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFR1282845.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(734..2158)
                     /gene="sbcB"
                     /locus_tag="ACHURJ_004141"
     CDS             complement(734..2158)
                     /gene="sbcB"
                     /locus_tag="ACHURJ_004141"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFR1282846.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIGE
                     PEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMPPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSD
                     TLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPTDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            2340..>2414
                     /locus_tag="ACHURJ_004142"
     CDS             2340..>2414
                     /locus_tag="ACHURJ_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282847.1"
                     /translation="MAISDTKLRSIYGKPYSGPQEVADA"
BASE COUNT          581 a          576 c          622 g          635 t
ORIGIN      
        1 gctgtactca tcggtttgat tgcgctttta gcctggcccc tgagtgaagc aaccggacgc
       61 atgtttgggt taggaatcac ttccccaacg gccaatattc tgcaatttct ggtcgcgggt
      121 gacgtgaaat acattaactg gggcgttttc ctggtgttag ggatcttcgt ggggtcattt
      181 attgcagcca aagcgagccg tgagttccgc gttcgcgcag ctgatgcaca aacaacatta
      241 cgtagcgggt taggtggtgt actgatgggc ttcggtgcca gtattgcagg tggttgctct
      301 atcggtaatg gactggttat gactgcaatg atgacctggc agggctggat tggccttgta
      361 tttatgattc tcggagtctg gactgcatcc tggcttgtgt atgttcgacc gcagcgtaag
      421 gcgcgactgg cgactgctgc ggcaaattaa gtaaggtgta aagatggcga ttaaaaagct
      481 ggatgtggtg acgcaggtct gtccgtttcc actcatagaa gcaaaagcag cactggcaga
      541 aatggccagc ggcgatgagt tggtaattga gtttgattgc acccaggcaa ctgaagcaat
      601 cccacagtgg gcggcagaag aaggacatgc catcaccgat tatcagcaga ttggcgatgc
      661 agcgtggagc atcaccgttc aaaaagcctg actcaacatt gtcctccgcc gtaccagcgg
      721 cggaggcttc aaattagacg atctcttccg cgtactgcca aagtgctttt aacagcgcca
      781 ctttttcttt gtcatcggca tattgttgta ccagcatctg caattcatca gcataaccct
      841 gcaaaaactc tggcgtgaat acctggcggc ggtgctccag ccagcgttgc tgctcggcat
      901 aatccagcgt ccccgggaag ttgcgtgccc gataattgaa caacagcttt tcaatccgtt
      961 tatcaacaaa agtgatatcc agtgccggta aattacgtgg ctcggtttcc agcacaattt
     1021 tcattgctgc acgatctgcg tcactgaaaa agccgttata aagctgtgca tccacgttat
     1081 ctgtaggcgt aaacggttcg gcttccgcga atatcgccac cactttttca cgcacttgcg
     1141 gattttcacg cagaattttc aggttatcga ggcaatgctg acgattaatt cccagtcggt
     1201 cggcatcttc cgggcgtagc gtattcgcct gggccagcac cggacattta ttgatgtgca
     1261 ccagcttaac cggaacggcg gcgttatcgc caagatcggt ttttgcggta tataaacgct
     1321 cgcgcaatgt gtcgctatcc agttccagta atggcgaaat gtctcctgcc aaatccacca
     1381 taattacagc attgcgattt tcaggatgcc acgccagcgg tgccacccag ctggtattgc
     1441 cgcgccatgc cccaaacatt ccggaaacgt gaaccagggg gggcatctgc ggaacatcaa
     1501 tcaacgccat cagtttgtgt ttattacgat gggtaaagag ataatcaaac aggcgtggct
     1561 gacgcgtttt taccagtttc gccatcgcaa tagtggcgta cacatcagcc atcgcatcgt
     1621 gggcgttgct atgttcaata ccattcgctt tggttaaatg ctcaaggcga aagctcggta
     1681 gaccgtcatc attttcaggc cagtttattc cttccgggcg cagggcataa caggcacgca
     1741 taacatccaa caaatcccag cgcgagttat catgctgcca gctccaggcg taaggatcgt
     1801 agaaattacg ataaaaaacg ttgcgtgtga cttcgtcgtc gaaacgcaca ttgttgtagc
     1861 ccagaataca ggtcttcggt acggtaaaaa gcgagtgaat acgggcggca aacgcggctt
     1921 cgttttctcc tttcgcccgt gcttcctgcg gggtaatacc ggtaattaat actgctccag
     1981 gctggggtaa atagtcatcc gcgggcttgc agtaaaagac ttcgggttcg ccgatgacat
     2041 tgaattcgct atcggtgcga atggctgcga actgtgcagg gcgatctaac gcggggtgcg
     2101 tgccaaaggt ttcgtaatcg tgaaagagga aggtaggttg tttatctgtg tctgtcattg
     2161 tctagcattg tccactgtag ttaattaact gtctgttata actcgaaaaa caaccatttt
     2221 ttcacctgag tatgtctacg aacgtcaaat atagttgttt actgtgcgct gttgtccatg
     2281 tgatattgtg taccagatgt gtaccaaacc attatttctg agcgtaccaa atattattta
     2341 tggctataag cgacacaaaa ctgcgtagta tttatggtaa accatattcg ggcccacaag
     2401 aagtggctga tgcc
//
LOCUS       DAZPEB010000312         2412 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZPEB010000312 DAZPEB010000000
VERSION     DAZPEB010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.318"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            42..551
                     /gene="eco"
                     /locus_tag="ACHURJ_004143"
     CDS             42..551
                     /gene="eco"
                     /locus_tag="ACHURJ_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFR1282848.1"
                     /translation="MKKESKKMKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     KGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAEVR"
     gene            complement(701..2347)
                     /gene="mqo"
                     /locus_tag="ACHURJ_004144"
     CDS             complement(701..2347)
                     /gene="mqo"
                     /locus_tag="ACHURJ_004144"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFR1282849.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMLERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
BASE COUNT          573 a          663 c          601 g          575 t
ORIGIN      
        1 atatgttaac cttcagcgac atcatcggtg aaaacctata aatgaagaag gaaagcaaaa
       61 aaatgaagac cattctacct gcagtattgt ttgccgcttt cgctaccact tccgcctggg
      121 cggcagaaag cgtccagcca ctggaaaaaa tcgcgcctta tccacaagct gaaaaaggga
      181 tgaagcgtca ggtgattcag ttaaccccgc aagaagatga atctaccctg aaagtagaac
      241 tgttaatcgg tcagacgctg gaagtcgatt gcaatttgca tcgtctcggc gggaagctgg
      301 aaagcaaaac gctggaaggc tggggctatg attattatgt ctttgataaa gttagctcgc
      361 cggtttccac gatgatggcc tgcccggatg gcaagaaaga gaagaaattt gtcaccgcgt
      421 atctgggcga tgctggaatg ctgcgttaca acagcaagct gccgatcgtg gtgtatacgc
      481 cagacaatgt agatgtgaaa taccgcgtct ggaaggcgga agagaaaatt gacaacgcag
      541 aagttcgcta aactgttgtg aagtacggca ccccgttggt tggataaggc ggttacgccg
      601 catccaacat ctaacgcccg agccggttgc ctgatgcgac gctggcgcgt cttatcagta
      661 tacgccacat ccggcatacc atgccggatg tggcacatca ttacaacgca atatccgcca
      721 cttctttttg aacgggttgc ggtttcaact gcggttttgg cgtactatct gctgcctgcg
      781 gcttgtcgta tttcagcccc agcacttcgc tggtgtactg caactcgcgt tctgttgccg
      841 ctacatcacc gttcagcttg cgtccataag acggaacgat cgctttcaac gtagcctgcc
      901 attgcgggct ggaaacgcga tcgccaaata ctttttccag cagatccaac ataatcggcg
      961 cggcggttga cgcccctggc gatgctccca ggagcgcggc aatggtccct tgctggtcac
     1021 tgaccacttc agtacccaga cgcagtacac cacctttatc ggcatcacgc ttgataatct
     1081 gcacacgctg ccccgcttgc cacaaacgcc agtcctcttt tttcgcctgc ggatagtact
     1141 ctttcaacgc ttcaaaacga tcctcttcac tcaacatcac ctgactcacc agatatttca
     1201 ccagatcgaa attatccagc ccgacgtgca tcatcggcat cacgttagag gtggtggtgg
     1261 aactcatcag atcccacaat gaaccgtttt tgaggaattt ggttgagaag gtggcaaatg
     1321 gcccaaacag cactacgcgt ttaccgtcca gaacgcgggt atcgatatgc ggaaccgaca
     1381 tcggtggtgc gccaacggat gctttaccgt aaaccttcgc cagatggtga ttaaccacgt
     1441 ccgggttttc cgaaacaagg aactgtccgc ccaccgggaa accggcgtag tctttcgctt
     1501 ccggaatccc cgattcctgt aacagcttca gcgccgcacc gcccgcgccg ataaagacaa
     1561 atttggcacg aatgttctgt gcagtgccat ttttcagatc ggcaacggta acggtccagg
     1621 tgttatcgtc attacgcttt agggcgcgga cttcgctgct gagttgcagc gagaagttag
     1681 atttcttctg caaggaagca attaactggc gggtgatctc gccgtagttc acatcggtac
     1741 caatttccgt acgcgtggct gccactttct gttgcggatc gcgcccttcc atcactaacg
     1801 gtgcccactc tttgatctgc gcgtgatctt cagagtaacg cataccgcga aacagcgagc
     1861 tttgttgcaa cgcggcgtaa cgggcgcgca ggaaattaac gttatcctcg ccccagacaa
     1921 agctcatatg cggaacggta ttgataaatg aacgcggagt acgcagcacg ccgcgctcga
     1981 cctggtgcgc ccagaactgg cgggaaatct gaaatgcttc gttaatggcg actgcttttt
     2041 caatactgat actgccatcg gcgttttgcg gggtgtagtt cagttccatc agtgcagaat
     2101 gcccggttcc ggcgttattc cagccgttcg aactctcctg cgcgacaccc tccaggcgct
     2161 ccagcatggt catcgaccat tcaggctcca gctcgcgtaa ataggtcccc aacgtggcgc
     2221 tcataatgcc gccgccaatc aacagtacat cagtttcctg ctcctcggac gctttcgctt
     2281 ttgccgccat cgaaacggca ttaagcccca cggccatcga gaagagcatg gcagtcactt
     2341 ttttcatctt gttaatgcct tacttttagt cgctttattg caggtgagat ttgcgcggca
     2401 tcaaaggtaa ca
//
LOCUS       DAZPEB010000313         2326 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZPEB010000313 DAZPEB010000000
VERSION     DAZPEB010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.319"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            272..538
                     /locus_tag="ACHURJ_004145"
                     /pseudo
     CDS             272..538
                     /locus_tag="ACHURJ_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233446.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(623..>2326)
                     /locus_tag="ACHURJ_004146"
     CDS             complement(623..>2326)
                     /locus_tag="ACHURJ_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HFR1282850.1"
                     /translation="LNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVSNSSELIQ
                     FKNKTAPYFSEKRNVKVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLLST
                     PDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACTLN
                     HQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDFYW
                     KIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEKYK
                     NGEISQSKLVNLLATRSSDGTPAFYIALQNGCSDIIQVYGKILNMCNLSQETILTLLA
                     AVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFFAL
                     QNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKILP
                     PHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSATN
                     KDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFNHF
                     L"
BASE COUNT          669 a          469 c          406 g          782 t
ORIGIN      
        1 aagcttgata caactggtct ggtatataca gtttctttga gtgcggttac gtccacagag
       61 gtaacggtaa aagtaacccc ttgtcgggac cactccgacc gcgcacagag gcattttcat
      121 cgctgacagg ccgcattgcc tcgcagggct ggaggaggag cggtgatatc cggacgaccg
      181 taccggctta cgccgggctt cggcggtttt actcctctgc catgtgcaac aacggagtgc
      241 cgccttacat gccgctggcg cggcatatcc tggtaacatt tttgttgata ccaccttccg
      301 gccttatcct gaccagtggg ctttcctggc atcgatgata ccgatgaacg cgcatgatat
      361 tgagccgacg atattacggg caactggaaa tactcaccct ctggatgtga cctttatcca
      421 tgaagaagat ttggccatac catggaaacc tgaacaatca tcagtttacg cccatgtaaa
      481 cccctacggg atatttgaac tcgatatgga aacaaggttg ccaatcgagg tggttgcctg
      541 aaagttggca gttacccggt cactacacct tgttggagtg aacatatcaa aaacaggtag
      601 ttacacaatt taaattacag tctcataaaa aatgattaaa tctatttatt atttcaataa
      661 acgttttttt catcttcttt gaaaattgtt ctgctatagt atccggttcc agattgttct
      721 cttttattat tctacaatac tctaatattg tttctttgtc tctatttact acagatacaa
      781 atacaccagg aataccatct ttattagttg cggacagcat ttcgaccaat cgtcttgtgg
      841 taaaactata gttttttaga atattacaaa aggcacgtat tgtttcggta tggccattat
      901 aaaatgccat aaaaagtcct gatgtaccgt tgggattttt tatccataat aattcatcta
      961 tatattcctg gtgagggggg agtattttta gtatatctcc atacgccata atggcctttg
     1021 tgtgcccatt ttgaagtgcc ataaacaacc catgctttac tttcaataat tctttaattt
     1081 gttcactact aagcattttc ttattaagca gacttccgaa catacgaata ctatctgcat
     1141 gtccattctg caatgcaaaa aataacccag gtaaatcatg gctgtctttc gcagctaata
     1201 gatataatct cttatcggaa gttaacgggg ttttaaagac tatttcccca taggctttta
     1261 ttgtgtctac atgtccgttc ataaaagaca tgcataaacc cggcacattg tttgccccta
     1321 ccgcagcaag caatgtaagt attgtttctt gactaagatt gcacatattt aatatcttac
     1381 catatacttg aataatgtct gaacatccgt tttgaagtgc tatataaaat gcaggagtac
     1441 catcagagct tctcgtggcc agaaggttca ccagtttaga ttgagaaatt tcaccatttt
     1501 tgtatttttc catcagtata aataatactt ctgtcagatt ctttgttatc gcatgatata
     1561 taactctgga attgatcaat tcatctggta aaagtttcac tgtgtttgtt agaggggaat
     1621 tatgcctatc ttcaaatatt acctgcccgg gttgctccag accataaatt ttccaataaa
     1681 aatcatcaaa aaaatcctta gcggataatt gactcagttc gaaatggcta tcacaagtaa
     1741 atacagtacg tttatgagta acagttcgat taggatcgaa aaactgaaca acccattttt
     1801 cactttcttg tacgcgcttt attttcaacc ctaacgccat ttggtggttt aatgtgcatg
     1861 ctgataatgt tctgacaaag tctccatttt ttttcatgtt ataaaaaaaa ttagtaataa
     1921 ctgttcccca ctttttattt tccacaaaat aaatataagc tggtgatttt gaaaaatctt
     1981 ccgttttttc caaacataca ttttttgcaa tagcatcagg ggtgctaaga agctgatagt
     2041 ttaccttccc atttgtttcc ataaaattca tttcccaata ggaagctagg tgacggcaaa
     2101 ctatttgtct tccatatata tcttttgcca ctccatttat atttaccttc acatttctct
     2161 tttctgaaaa ataaggtgct gttttattct taaactgaat tagttcactg gagtttgaga
     2221 ccctcataat agaacttgtt attgttctat tatctctgtt aacagacata tctgttgata
     2281 acgaattttt tgttgaaaat atactagatg aaaatgtttt atttaa
//
LOCUS       DAZPEB010000314         2326 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZPEB010000314 DAZPEB010000000
VERSION     DAZPEB010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.320"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..465
                     /locus_tag="ACHURJ_004147"
     CDS             <1..465
                     /locus_tag="ACHURJ_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005166249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282851.1"
                     /translation="LPISAADKRRFYYYFQEKNTPNIERFFVFDSSEYRYALNMREVV
                     FHQFLSDGLRPIVDEDDDAYEDDYFNVHITLVNGGPVIPLSVEPDAPQNEETDDIGQL
                     NAFFDALDCEPETTDRFMITDEDGEDAFIRIGSIAMVRVALDVLEPVEDDDE"
     gene            712..1977
                     /locus_tag="ACHURJ_004148"
     CDS             712..1977
                     /locus_tag="ACHURJ_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001642584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1282852.1"
                     /translation="MALTDIKVRAAKPTDKQYKLTDGGGMHLLVHPNGSKYWRLQYRY
                     EGKQKMLALGVYPEITLADARVRRDEARNLLANGVDPGDKKKNDKVEQSKARTFKEVA
                     IEWHGTNKKWSEDHAHRVLKSLEDNLFAALGERNIAELKTRDLLAPIKAVEMSGRLEV
                     AARLQQRTTAIMRYAVQSGLIDYNPAQEMAGAVASCNRQHRPALELKRIPELLTKIDS
                     YTGRPLTRWAIELTLLIFIRSSELRFARWSEIDFEASIWTIPPEREPIPGVKHSHRGS
                     KMRTTHLVPLSTQALAILKQIKQFYGAHDLIFIGDHDSHKPMSENTVNSALRVMGYDT
                     KVEVCGHGFRTMACSSLVESGLWSRDAVERQMSHMERNSVRAAYIHKAEHLEERRLML
                     QWWADFLDVNRERFISPFEYAKINNPLKQ"
BASE COUNT          660 a          452 c          569 g          645 t
ORIGIN      
        1 ttgcccattt cagcagcgga taagcggagg ttttattatt attttcagga aaaaaacacc
       61 ccaaacatag aacgcttctt cgtttttgat tcttccgagt atcgttatgc gttaaatatg
      121 cgtgaagtgg tatttcatca gttcctttct gatggtctgc ggccaatcgt tgacgaagac
      181 gatgatgcct acgaagacga ttattttaat gtccatatca cgctggtaaa cggcggacca
      241 gtgattccac tgagtgttga gccagatgcg ccgcaaaacg aagagacgga tgatatcggc
      301 cagttgaatg cattttttga cgcgctggat tgcgagcctg aaaccacgga tcgttttatg
      361 ataaccgacg aagatggaga agatgctttc attcgtattg gttctatcgc tatggtgcga
      421 gttgcgcttg atgtgctgga accagtggaa gacgatgatg agtagtattg aatggattga
      481 tttcatattg ttgagtctgg aatcgcggtg cggccttcgg caccaaaagt atgtaaatag
      541 acctcaactg aggtcttttt ttatgcctga aatccagtgt ttatctatct ttcccgctat
      601 attaactctc tcaaggtcaa ccgatatcaa cgtacatcta ccaacatatg ttggtacaga
      661 tgatggtatt tccggttcga taatgcttgt accaacaggg agggaatacg catggcatta
      721 acagatatca aagtcagagc agccaagcca acggataagc aatataagct gactgatggt
      781 ggcggtatgc atctgcttgt ccatccaaat ggttctaagt actggcgttt gcagtaccgt
      841 tatgagggaa agcaaaaaat gctggcactt ggggtttatc ctgaaatcac actagcggat
      901 gccagagtac gtcgtgacga ggcgcgtaac ctgcttgcga atggcgtcga tccgggagac
      961 aaaaagaaaa atgataaggt tgaacagagt aaagcacgaa cctttaaaga agtcgcgatt
     1021 gagtggcatg gcaccaataa aaagtggtct gaagatcacg cccatcgtgt gctaaaaagt
     1081 cttgaagata atctttttgc agcgcttggt gaacgtaata tcgctgagtt aaaaactcga
     1141 gatttattag cacctattaa ggccgtagaa atgtctggac gtcttgaagt ggccgctcgt
     1201 cttcagcagc gcactacagc catcatgcgc tatgcagtgc aaagtgggtt aattgattat
     1261 aacccggcac aagagatggc tggggcggtt gcttcctgta atcgacaaca tcgtcccgcg
     1321 cttgaattaa agcgcatccc tgagttgctt acaaaaatag atagctatac tggtaggccg
     1381 ctaacccgat gggcgataga actcactttg ctgatcttta ttcggtccag tgagctgcgt
     1441 tttgctcgtt ggtcagagat cgatttcgaa gcgtctatat ggactatccc accggagcgg
     1501 gagcctattc ctggagtgaa acattcccat agaggctcaa aaatgcgtac aactcatcta
     1561 gtgcctcttt caacgcaagc tcttgcaatt ttaaagcaga taaaacagtt ttatggggcc
     1621 catgacttga tatttattgg tgatcacgat tcgcacaaac ccatgagtga gaatacggta
     1681 aatagtgcgt tacgggtcat ggggtatgat acaaaagtag aggtttgtgg tcatggcttt
     1741 cgaacaatgg cctgtagttc attggtcgaa tcaggtttgt ggtctcgtga tgctgttgaa
     1801 cgtcagatga gccacatgga gcgaaattca gtgagggccg cgtatatcca taaagcagag
     1861 catctggaag aacggcgatt gatgctacag tggtgggccg attttctgga tgtaaacaga
     1921 gaaagattta tcagtccatt tgaatatgca aagattaata atccattaaa acagtaatca
     1981 tcccgggcaa atgcccggga attattctag gattattttc tttgttaaaa aagacaaacg
     2041 gtattaactg atgtatttac tatttaccgc tccctgcagg ggggattatc agaatccgcg
     2101 gttattcatt atttatatac tttcaataaa aaagtaacct cataaaaaat cctgtactca
     2161 cagggtgatg cgggataact gacgtaagga gtgctgcagg gatttgatgg tcccttgtgc
     2221 tgatatgaat acggatattt tatacagaat acttttcagt acaagcgact gtattacagt
     2281 ctgccatata tgattacctg tgatcaggaa gaataagtgg ctgagg
//
LOCUS       DAZPEB010000315         2307 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZPEB010000315 DAZPEB010000000
VERSION     DAZPEB010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.321"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..377)
                     /locus_tag="ACHURJ_004149"
     CDS             complement(<1..377)
                     /locus_tag="ACHURJ_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm57 family immunity protein"
                     /protein_id="HFR1282853.1"
                     /translation="MNNCIFFSISASAVTARQFQRELDLADKAILWALISASTKEERK
                     ACSLSYFACKAAEAELGLAYMAANDNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGK
                     IIRPYLKNLNPLQLAADCIETVN"
     gene            complement(1013..1474)
                     /locus_tag="ACHURJ_004150"
     CDS             complement(1013..1474)
                     /locus_tag="ACHURJ_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HFR1282854.1"
                     /translation="MKACLLLFFYFSLIDQLHGADVKIKENESVMGSTAMTYDLSEEK
                     LMKLKYKSQHGDSEASFRLYQYYCFTKNNIDKQLRFLERSASQGNVTAQFNYGVFLSD
                     TNPTLSEYYNLNRAIYWMEFAVNNGNIDAKSKLQELKKLKRMDRRKNKENP"
     gene            complement(1486..>2307)
                     /locus_tag="ACHURJ_004151"
     CDS             complement(1486..>2307)
                     /locus_tag="ACHURJ_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFR1282855.1"
                     /translation="YTPARKIHLYHCDHRGLPLALISTEGATAWCAEYDEWSNLLSDE
                     NPHHLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNFYQYPLNP
                     VINVDPQGLVDINLYPESDLIHSVADEINIPGVFTIGGHGTPTSIESATRSIMTAKDL
                     AYLIKFDGNYKDGMTVWLFSCNTSKGQNSFASQLAKELHTNVIGPDTLWTWWGRGTNG
                     KLKMDTVLTAPTNLNSNKDLMAITTKDLGNWITYGPSGHPISNMQGTPEKPSDIR"
BASE COUNT          643 a          501 c          415 g          748 t
ORIGIN      
        1 ttatttactg tttcaatgca atcagcggca agttggagtg gatttagatt tttcagatat
       61 ggtcttatga tctttccctt acttaataaa tagcatgtat aggattcgga aagtccagca
      121 tctattttgt acctcattat attagataag gatgtgagaa attccttgtt atcattcgca
      181 gccatatatg ccagtccgag ttcagcctct gcggccttac aggcaaaata gcttagtgaa
      241 cacgcttttc gttcctcttt agtactggct gatattagtg cccataatat tgctttgtct
      301 gccaaatcta actctctctg aaattgtctt gcagtaactg cactcgcaga aatagagaaa
      361 aaaatacaat tattaataac cttgccatat ttttttagtc cgtaactgtt aatgtgctat
      421 ctggctctga aaatattaac tcatttaatt tttgcatgtc attagataaa ccagttatac
      481 aaccctcgga acagaatcga ggtcctaaag acggaccaaa agcgtttaaa caactatgta
      541 ttgaaaatcc attgcgatta taagttcctg tattaactga cggtactaat cgtatgccgt
      601 caggttttct attgcttgct cccgggtcat taacattcca actccatagg ccttgaggta
      661 ttggccatct atttataaat gtgtacaacg gggattattt ttacattgca tattattacc
      721 agaagcgact ggaatactaa gcacatcata aaaattatca tcacatggta tatctgaaac
      781 acacgaaagc atacctaaag ttatgctgta taaacaggtg gctaatccca aggggatcga
      841 ttccagaaat cgggttcaac ggatactgat aaaaattcca cctaccttcc agcccaatcg
      901 gagcctgagt gttataccgc ccctgctgcg gttaatgtta ccgataatag ttatagtaca
      961 gccctgattc atcatcatac tactgctgcg gcagtctgat gaactattgc ttttacggat
     1021 tctccttatt cttccttcta tccattcgct ttagcttttt gagctcttga agtttgctct
     1081 ttgcatcaat attaccgtta tttacagcga attccatcca ataaatagct ctattcaaat
     1141 tataatattc tgataatgtt ggatttgtat ctgataaaaa gaccccataa ttaaactgcg
     1201 ctgtaacatt cccctgagat gctgatcttt ccaagaatcg caattgttta tcaatattat
     1261 ttttagtaaa gcagtaatat tgatatagtc gaaatgatgc ctctgaatca ccatgttgtg
     1321 atttatactt cagtttcatc agcttttctt cacttaaatc ataggtcatt gctgtagatc
     1381 ccatcacact ttcgttttct tttattttca catcagcacc atgcaattga tcaataagag
     1441 aaaaataaaa aaatagtaac aagcatgctt tcatacatct acaacctatc ttatatcact
     1501 gggtttttct ggcgtacctt gcatattaga aatggggtgc ccagatggcc catatgttat
     1561 ccaattacca aggtcttttg ttgttatagc cattagatcc ttatttgaat taaggttcgt
     1621 tggtgctgtt agcactgtat ccatttttaa cttaccatta gttcctcgcc cccaccacgt
     1681 ccatagcgtg tcaggtccta ttacatttgt atgtaactct ttagctaatt ggctagcaaa
     1741 tgaattttgt cctttacttg tattacaaga aaataaccaa actgtcatcc catctttata
     1801 attcccatca aatttaatta gatatgctag atctttagct gtcatgatac tgcgcgttgc
     1861 ggattcaata gatgtggggg taccatgccc cccgattgtg aaaacgcctg ggatattaat
     1921 ctcatcagct acagaatgga taagatcact ttcggggtat aaatttatat caaccaaacc
     1981 ttgcggatct acatttatga ccggattcaa cggatactga taaaaattcc atcccccctt
     2041 cagcccaatc ggatcctgag tgatataccg cccctgcagc gggtcataat agcggtggcg
     2101 gttgtaatac aggccggact cctcatcata ctgctgaccc ggcaggcgaa tgagctgctg
     2161 cagatgatgc gggttctcat cactcagcag gttgctccat tcatcatatt ctgcgcacca
     2221 cgctgttgcc ccttccgtgc tgatgagcgc cagcggcagg ccgcgatggt cgcagtggta
     2281 caggtggatt tttcgcgccg gcgtgta
//
LOCUS       DAZPEB010000316         2281 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZPEB010000316 DAZPEB010000000
VERSION     DAZPEB010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.322"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..689)
                     /locus_tag="ACHURJ_004152"
     CDS             complement(<1..689)
                     /locus_tag="ACHURJ_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFR1282856.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSA"
     gene            complement(1050..2054)
                     /locus_tag="ACHURJ_004153"
                     /pseudo
     CDS             complement(1050..2054)
                     /locus_tag="ACHURJ_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            2039..2281
                     /locus_tag="ACHURJ_004154"
                     /pseudo
     CDS             2039..2281
                     /locus_tag="ACHURJ_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          551 a          528 c          581 g          621 t
ORIGIN      
        1 cttgcgctta acaagtcggg attatagttt tgtccaaaca gatattgttt gccgtttaat
       61 atcgttgagg ttgttccggc acctgctctg aagccaatta tttgttcctt accgttgtag
      121 tttacgccat atcttttcat cgcatcttcg ggagaaagtg agaagtcggc actctccgta
      181 acccctgttg tttccacgac gaatttattc agacgatgaa ccgaaaagtc gccagatgat
      241 actctgttac ttgctttata tgctgtctga tcccatgact gtgttgttat tgcatgatgc
      301 tgtgtaccgt tatgagtcgc agtaacactg tacgcacctc caattgatgt tgttgcacct
      361 ttgttggaaa cagcagaaaa atcaggtaca ggtagttctg gaaaatgaaa tatctttcca
      421 tctttcatca ttaattgaac atttgttgca cccggtctga actctcctct gttttgagca
      481 aggtcaagat aatcgcgggt ccatatatta tttatatcca gcattgctgc aaatgctgtt
      541 tcaccataac ttccatatat tgaggataaa ataataccga ggataacttt ctttcttgat
      601 gtaccgacac taactttccg ggtcagttca gaaacagcaa ctaatccacc tgtaatatga
      661 ctatatttca gtgaataaat tttattcata cgtgaactcc gatatttctg agttgtggtt
      721 atgggatagc atttaattac aacggggaac tatcagtcat ggtacgtaac tgataagggt
      781 ataatgataa tcactgtcag ttatatttgt atgacagatg taacaaaatg aaaaaaaaga
      841 taagcacaag tgattgattg gtcagatatt gatcacctgg tgtttgtatt ttgacctttg
      901 cgatcaaaat ttgatctttg ttgtttccgg tcaatccttt acaggttttc tgtattatcc
      961 gggggagtta agtctgtcga tgtccgacag acttttgatg atggaaacaa catgcagcga
     1021 taatgtgcat gctgttttgc aggtggaact cagaaggtga actggaactg tgcgaccgca
     1081 ccgtaagtgt tgtccgtgcc gatcaggcct gcaacatcaa ggtgaaccac atcaaacggt
     1141 gagatcccca ccccggccgt gaatgcactg ccgtcgttac cggccagatt ctgacggtaa
     1201 ccggcgcgca gctgtgccca tttccaggca ttaaattctg cgccaatggc ggcaaactga
     1261 cggttgctgt cggaggtgaa accgctggcc ggggtcagat ccacatcaaa tgcggtggtg
     1321 aacatcgcgt tgtgccagga gacaccggcc gtcacctgcg ggcggatcct gaaggtttcc
     1381 gtgataccgt tcacttcttt tgtctcaata ctacggggga tcaggttctg tgcgaccagt
     1441 cccagcgtcc agttgtcatc caggttcgta ctggcaccga tgtcggcgtt gatgccattt
     1501 ttcgtgttgt ggtagcggtc gcctttgaag gcgctgctgt cataatttct gaccagcacg
     1561 ttgtagttga acaggtcgac gcgctggaat ttggggggga agcccagaga ccatttctgc
     1621 ccggccgttt ccagttccgt agccagagca atacccacat cagtgatcag tgccgcgcgg
     1681 gcaaaggcct gtgatgtcag gcggcttttg tccacctcct gcccggtgct gtttgccacc
     1741 tcttccaggt aagtgaggtc agcatcagag actttaccgt cgacactgac ggtgccgtgt
     1801 gatttaacca tcagtgcggc agagaggcga tcaccgggaa gggccgccac agcagaaacg
     1861 cccagctgtg cggcggcatg tgtgttacgg aagtcccgga ggtgttcttt caggcgtgcg
     1921 gcagcctcag gaacaccgtg gttactttcg atggcgcggt cgaaggcctt ccactcatca
     1981 ctgatccggt cggcgttgtc ggtgatatta tccggattgg agagctgcgc gccgacggac
     2041 ggaagcagga tgctgggctc atgcgcgacg caaaatccac gatgtatata tcagtactaa
     2101 aagcgcgacg gcggaagaag ctctgaaact aatcggtgaa ctgtacgcca tagagcacga
     2161 tatacgcggg ttaccggtgt ctgaacgcct ggcggtcagg caaatgcaga gtagaccgct
     2221 cctgacttcc ctgtataagc tgatgcagga gaaagaacac acgttatcga agaaatgtcg
     2281 t
//
LOCUS       DAZPEB010000317         2277 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZPEB010000317 DAZPEB010000000
VERSION     DAZPEB010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.323"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..350)
                     /gene="pliG"
                     /locus_tag="ACHURJ_004155"
     CDS             complement(<1..350)
                     /gene="pliG"
                     /locus_tag="ACHURJ_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFR1282857.1"
                     /translation="MKIKSISKAVLLFALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRN"
     gene            592..885
                     /locus_tag="ACHURJ_004156"
     CDS             592..885
                     /locus_tag="ACHURJ_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFR1282858.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            957..1616
                     /locus_tag="ACHURJ_004157"
     CDS             957..1616
                     /locus_tag="ACHURJ_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFR1282859.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1708..2154
                     /locus_tag="ACHURJ_004158"
     CDS             1708..2154
                     /locus_tag="ACHURJ_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFR1282860.1"
                     /translation="MNDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PVWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
BASE COUNT          592 a          491 c          588 g          606 t
ORIGIN      
        1 tcgtttcggg tttgcagcac tcgcagctca tatttgccag aagcagggag tgagtattgt
       61 ccgtgactat cgagttctgg ggaatatctg gataaatcaa cagaatcatc gattcctggc
      121 ccgaagaggt aagtatcagc gccttcatta gagatactca catgtacttt ctgacctttt
      181 ttggcataga aggtatatgt atcgtaatcg tatcccttta tttcgccgga gtactgagcg
      241 ctgctatggc cttttctgaa ctcaacattg acattcttac ccgcagcaaa gctcgtggag
      301 gttagcagtg caaacagcaa tacagcctta ctgatgctct tgattttcat tggtagtctc
      361 ctgttcagaa tgagtacgat gaactgttat aatataacaa tccctaacgg gagaattcca
      421 gtggcttttc tgcgttcggt aaaatcccgg cgcggcattt tttgccgggg ggattactta
      481 taaaggggca tggactcaac aaaccgcaga aatctacagc tggctggctt gtggcgcggg
      541 tttcattgat gttagcttat gccaaaacct ggcatcttaa agagtaaaag tatgttttgt
      601 gtgatttatc gaagcagcaa gcgtgaccag acctatttat atgtcgaaaa aaaagacgat
      661 ttttcgcgtg ttcctgagga actgatgaaa ggttttggtc agcctcagtt agcgatgatt
      721 ctgccgttgg atgggcggaa gaaactggtc aatgctgata ttgaaaaggt taaactggca
      781 ttaaccgagc aaggttacta tttgcagtta ccgccaccac ccgaagattt gctgaagcaa
      841 catctttccg tcatggggca gaaaacagac gacacgaaca aataaccgat atccggcggt
      901 ggcattatct ttgtcggcgc gggttttcat atccacgata aggtgagggg aacgttatgt
      961 atcaacatca caactggcaa ggtgcgctgc tggattatcc ggtgagtaaa gtagtctgtg
     1021 ttggcagtaa ctatgccaaa catattaaag agatgggcag cgcagtgccc gaagagccag
     1081 tgctgtttat taaaccagaa acggcactgt gcgatctgcg gcagccattg gcgatcccat
     1141 ccgatttcgg ttcagttcat catgaagtcg aactggcggt gttgattggt gcgacgctgc
     1201 gtcaggcaac ggaagagcat gtccgcaaag ccattgccgg ttacggcgtg gcgcttgatc
     1261 tgacattgcg tgatgttcag ggaaaaatga agaaagccgg gcagccgtgg gaaaaggcta
     1321 aagcgtttga taactcttgt ccgctttccg ggtttatccc cgcggcggaa tttaccggcg
     1381 atccacaaaa tacaacgctg ggcctgagcg taaacggcga acaacgccag caaggtacga
     1441 ctgcggacat gatccataaa atcgttccgt tgatcgctta tatgagcaag ttttttaccc
     1501 tcaaggccgg tgacgttgtg ttgacaggca cgcctgatgg cgtcggcccg ttgcaaagcg
     1561 gtgatgagct gacagtcact ttcgatgggc attctttgac aactcgcgtt ttgtaatact
     1621 ttttgccgcc tgaaagcggc ggcaaaactt gcatcgctgt gccagactgg ttataaggtg
     1681 cgttttaacg taatggcgga acacctgatg aacgatgtac ctttctggca aagtaaaacc
     1741 ctggacgaaa tgagcgatgc ggaatgggag tcgttgtgtg atggttgcgg tcagtgttgc
     1801 ctgcataaac tgatggatga agacaccgac gaaatctact tcactaacgt cgcctgtcgc
     1861 cagctcaata ttaaaacctg tcaatgtcgg aactacgaac gtcgtttcga gtttgaaccc
     1921 gactgcatta aattaacccg tgaaaatctg ccaacattcg aatggctgcc aatgacctgc
     1981 gcttatcgtt tgctggcgga aggtaaagat ttacctgtgt ggcatccgct acttactggt
     2041 tcgaaagcgg caatgcatgg tgaacgtatc tctgtgcgtc atatcgcagt gaaagaatca
     2101 gaagtgattg actggcagga tcatatctta aataaacctg actgggcgca gtgataaatt
     2161 taatagatgt tgatgggggc tttcttaata tacgtctgga tgctcgccat ttcgtagccc
     2221 tgtttttgaa agcgctctgt cataatgaga gttacccgat accttattat caacagg
//
LOCUS       DAZPEB010000318         2265 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZPEB010000318 DAZPEB010000000
VERSION     DAZPEB010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.324"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..1241
                     /locus_tag="ACHURJ_004159"
     CDS             <1..1241
                     /locus_tag="ACHURJ_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFR1282861.1"
                     /translation="TNVKLIPQMNYLMVVVALFFLNAVIFLFMLMKYFTNKQILPTLI
                     LSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVSIFYIFRQLSFICLTSLALFCY
                     GKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYNADYSLYVVDYCPDNHTATRGI
                     NYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTLDEYNYTIWYI
                     SRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLTNIYNRRYFFNSVESLLSRPVV
                     KDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQQSIRPDDILARLEGEVFGLLF
                     TELNSAQAKIIADRIRKNVELLTGFSNRYDVPEQMTISIGTVFSTGDTRNISLVMTEA
                     DKALREAKSEGGNKVIIHHI"
     gene            1422..>2265
                     /locus_tag="ACHURJ_004160"
     CDS             1422..>2265
                     /locus_tag="ACHURJ_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAPES1 domain-containing protein"
                     /protein_id="HFR1282862.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGREVAK
                     FSVPL"
BASE COUNT          595 a          502 c          477 g          691 t
ORIGIN      
        1 acacaaatgt aaaactcatc ccacagatga attacctgat ggttgttgtg gctttgtttt
       61 tccttaacgc cgtcattttt cttttcatgt taatgaaata tttcactaac aaacaaattt
      121 taccaacact cattttaagc cttgcattct taagtggcct tatctattta gttgaaacca
      181 ttgtaattat ccataaacca attaacggca gtacactgat ccagacaaag tcgaatgatg
      241 tttctatttt ctatattttc cgccaactca gttttatttg tttaacctcg ctggcactct
      301 tttgttatgg aaaagacaac atccttgaca acaataagaa aaaaacggga atcctgttgc
      361 tggcgttgat tcctttttta gtttttcccc ttctggcaca caatctgagc agttataacg
      421 ctgactattc tttgtatgtc gtcgactact gtccggacaa ccatactgcg accaggggaa
      481 tcaactatac aaaaatattg gtttgtctgt gggcattttt actgttcttt attatcatgc
      541 gtacacgatt agccagcgaa ctatggccgt taatagcatt attatgtctg gcatcgctat
      601 gctgcaactt acttctactg actctggatg agtataatta caccatctgg tatatcagtc
      661 gcgggattga agtttccagt aaactgtttg ttgtgtcttt tctgatttat aacatttttc
      721 aggagctgca actctccagc aaactggcag ttcatgatgt gctgaccaat atttataatc
      781 ggcgctactt tttcaacagc gtagagtcat tattgtcgcg acctgttgtt aaggacttct
      841 gtgtcatgct ggttgatatt aatcagttca aacgcatcaa tgcccaatgg ggacatcgtg
      901 tgggtgataa agtgctggtt tcaattgtcg atattatcca gcaaagcatc cgccctgatg
      961 atattttagc gcgactggag ggtgaggtgt ttggcttgct atttaccgaa ctcaatagtg
     1021 cccaggcaaa aatcattgcg gatcgtatac gtaaaaatgt cgaactcctg accggcttta
     1081 gtaacagata tgatgttcct gaacaaatga ccatcagtat tggcacggtt ttttcaacgg
     1141 gtgacacgcg taatatctcg cttgtcatga cggaagcaga taaagcctta cgcgaagcga
     1201 aaagcgaggg gggcaacaaa gtgattattc atcatattta agtgcaaaaa tattcagagc
     1261 catgcttttt gcgtggcttt tgcatacaat ttattactat tacccctaaa tttcccctcc
     1321 ccagcggcgc agagatgagt ataattagcg cccctgtgcc aggccgcaat cgaactttat
     1381 ctggttttct cgtttcacta actgaaggag tgccatttat catgaaattg caccatagaa
     1441 tgctccggca ttttatcgcc gcaagtgtca ttgtgctgac atcttccttc cttatttttg
     1501 aacttgtcgc cagcgacaga gcaatgagtg cctatctgcg ctatatcgtg cagaaagcag
     1561 actcctcctt tctttatgat aagtatcaga atcagagtat tgccgcgcat gtgatgcgcg
     1621 ctctcgctgc tgagcagtcg gaagtgtcgc cagaacagcg gcgcgccatc tgcgaggctt
     1681 ttgagtctgc caataatacc catggcttaa acctgactgc tcataaatac ccgggcttac
     1741 gcggcacact acaaaccgca tccactgact gcgacacaat tgtggaagct gcagcactat
     1801 tacccgcttt tgatcaggca gtggaaggca accgccacca ggatgattac ggttcaggtc
     1861 ttgggatggc cgaagagaaa tttcactatt atctcgatct caatgaccgc tatgtctatt
     1921 tttatgagcc ggttaatgtt gaatactttg cgatgaataa ctggtccttc ctgcagtcag
     1981 gaagtattgg tatcgatcgc aaagatattg aaaaggtatt taccgggcgt accgtattgt
     2041 cgagcattta ccaggatcag cgtactaaac agaacgtgat gagtttgctg acgccggtgt
     2101 atgttgcagg gcagctaaaa gggattgtgc tgctggatat taacaaaaac aatttgcgga
     2161 atatctttta cactcatgac cgccctctcc tctggcgttt tctcaatgtc acgctaaccg
     2221 ataccgattc ggggcgtgaa gtggccaaat tcagtgtgcc acttc
//
LOCUS       DAZPEB010000319         2260 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZPEB010000319 DAZPEB010000000
VERSION     DAZPEB010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.325"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            72..1019
                     /gene="icsP"
                     /locus_tag="ACHURJ_004161"
     CDS             72..1019
                     /gene="icsP"
                     /locus_tag="ACHURJ_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HFR1282863.1"
                     /translation="MKLKFLVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGNRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVRSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(1581..1953)
                     /locus_tag="ACHURJ_004162"
                     /pseudo
     CDS             complement(1581..1953)
                     /locus_tag="ACHURJ_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
BASE COUNT          644 a          480 c          490 g          646 t
ORIGIN      
        1 gcttggcatt gacgtttatc ttataaagta agaagatcat ggacatttca accaaaaaag
       61 tagagttctc gatgaaatta aaattccttg tacttgcact ttgtgtacct gcgatcttta
      121 ctacacatgc taccactaac tatccacttt tcataccgga taacatcagt acggatatta
      181 gtctaggatc tctgagtggc aaaacaaaag aacgcgttta tcatcccaag gaagggggga
      241 gaaaaattag tcaactggac tggaaataca gtaatgcgac tattgttaga ggtggcatcg
      301 attggaagct aattccaaaa gtgtctttcg gagtttccgg ttggactact ttaggtaacc
      361 agaaagcaag catggttgat aaagactgga acaattccaa tacccctcag gtatggaccg
      421 accagagctg gcatcccaat acgcatctcc gtgatgctaa cgaattcgag ctgaatctta
      481 aaggttggtt attaaataat ttggattatc gacttggact aatagcaggt taccaagaga
      541 gtcgttacag ttttaatgca atgggaggga gttatattta tagtgaaaac ggggggaaca
      601 ggaataaaaa aggggcacat cctagtggtg aaagaacaat aggttacaaa cagctcttca
      661 aaatacctta tattggatta actgctaatt accgccatga gaattttgag tttggagcag
      721 aactgaaata tagtggttgg gttcgttcat ctgatacaga taaacactat cagactgaga
      781 caatttttaa agatgaaata aaaaaccaaa attactgctc tgttgctgcg aatattggat
      841 actatgtcac ccccagtgca aaattttata tagaaggctc cagaaattac atttctaata
      901 aaaaaggtga tacatctctt tatgagcaaa gtaccaatat atctggcacc attaaaaata
      961 gtgcaagtat tgaatatatt ggttttctca cttccgcagg tataaagtat attttttgaa
     1021 ttggttcacc gagttgatta cgttttcatg gctaaaaatt gccgcccatt gtgagtcata
     1081 ttcgccctga ctttccagaa ccatacgaac tgcccgttga cggacttcgg gggaaaaacg
     1141 tgtattttta gtcattctgt ttacctcttt ctcagggcgt ttagtctcca ggatttccgg
     1201 ggcggttcaa aaaactcttc gttgattact gtggacttac tgttggcatt accgaccctg
     1261 agaccggaga aataagaacc gcttagagca tcgtggctgt tctcggggta tcaagttaca
     1321 catgagcaga ggctacccaa ggtctactat tatcgcctga aagcgctgat ggaacagtgc
     1381 tatcagggac atgctgatga aaatacctaa ataatcagcc agcactctat ctttccaaat
     1441 ccacagcata gcaaagagag caaaagagcc tgtaaattca gaaattaagg aaatgcctct
     1501 tggtgcacat aatctatctt atgttaaatg aaatctaatt tcttgattat atgctggatc
     1561 gttcgtgggt ggctttgccc ctatatttcc agacacctgt tatcattggc ccgctgccgc
     1621 agatattccc gtggcgagcg ataatccagc gcactatgcg gatgccattc gttataatgc
     1681 tcgaatgcct ctgcaaggtt ctttgctgcc gttaacccgt caggtttggg catgatactg
     1741 atgtagtcac actttattgt tttcacgaag ctctttgcta ttctgttact ctccggactc
     1801 cgcaccgccg tgttcttcgg ttcaacaaag tcccaacatc cgggtgaact ggcgtgtttc
     1861 attagcccgg tagcatgaac cattatccgt cagtcacttc actggagacg ccggaagctc
     1921 gttgccgaag cggtgttcca ccgctcccag catgtcgcca acgggagagc atctacggtc
     1981 tgattgaact gtttattcaa attgtccacc gtctgtcagt acgctctgaa cgtcggctgg
     2041 ttaaaacact gctcgctgat tttcagaggg ttcacggtaa aacagcactt ttattccgga
     2101 ttgcggaagg gctctgaata atccggatgg gctggttaaa gaggttgtgt accatctgca
     2161 tattgttgaa ccagatcgca gctggatttg cccctatatt tccagacatc tgttatcact
     2221 taacccatta caagcccgct gccgcagata ttcccgtggc
//
LOCUS       DAZPEB010000320         2260 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZPEB010000320 DAZPEB010000000
VERSION     DAZPEB010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.326"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            111..308
                     /gene="yecR"
                     /locus_tag="ACHURJ_004163"
     CDS             111..308
                     /gene="yecR"
                     /locus_tag="ACHURJ_004163"
                     /inference="COORDINATES: protein motif:HMM:NF025358.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR family lipoprotein"
                     /protein_id="HFR1282864.1"
                     /translation="MRKSLMLLLASTLIISGCATKKQLVPTGGSKSDGTVRMSYSYGM
                     FESPVVDPQQGMNAAKARCAA"
     gene            280..432
                     /gene="yecR"
                     /locus_tag="ACHURJ_004164"
     CDS             280..432
                     /gene="yecR"
                     /locus_tag="ACHURJ_004164"
                     /inference="COORDINATES: protein motif:HMM:NF025358.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR family lipoprotein"
                     /protein_id="HFR1282865.1"
                     /translation="MQPKRVVPLRGYSGAEPFGGFTSQCSQPSSSGCMQTLVTIEYQC
                     TGDIKK"
     gene            complement(466..723)
                     /locus_tag="ACHURJ_004165"
     CDS             complement(466..723)
                     /locus_tag="ACHURJ_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282866.1"
                     /translation="MVSIVYNNLTIPLHEVDMTVSYKITYEHRDGDQIRMEEVLFHSE
                     HEPTKDEIHDAVRKDTVRFNSPSNGVTAVSGFKVISVVPVP"
     gene            complement(1170..1243)
                     /locus_tag="ACHURJ_004166"
     tRNA            complement(1170..1243)
                     /locus_tag="ACHURJ_004166"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1209..1211),aa:Gly,seq:ccc)
     gene            complement(1322..2077)
                     /gene="actS"
                     /locus_tag="ACHURJ_004167"
     CDS             complement(1322..2077)
                     /gene="actS"
                     /locus_tag="ACHURJ_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFR1282867.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKTGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            complement(2074..>2260)
                     /locus_tag="ACHURJ_004168"
     CDS             complement(2074..>2260)
                     /locus_tag="ACHURJ_004168"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282868.1"
                     /translation="NRVKSQEVRYLYSEYFADDTSLCQMKLTSFSITTFFQKQEIGYQ
                     EAGCYDKASCFLSEERF"
BASE COUNT          603 a          530 c          491 g          636 t
ORIGIN      
        1 tcatacacta aatcagtaag ttggcagcat taccaatttt gctaagttaa tgcattaatc
       61 taaaaacgct ttaaatcaat acataaccat tatctcttgg gattatttac atgcgcaaaa
      121 gtttaatgct gttacttgct tcaacattga ttatttctgg atgtgcaaca aaaaaacaac
      181 tcgttccaac tggtggaagc aaatcagacg gcacagttag gatgagttat tcttacggta
      241 tgtttgaaag tcctgttgta gatccacaac aaggcatgaa tgcagccaaa gcgcgttgtg
      301 ccgcttaggg gatattcagg agccgagcct tttggtggat tcacttctca atgttcacaa
      361 ccttcttcat ctggttgtat gcaaacactt gtgactatcg aatatcaatg cactggcgat
      421 attaagaagt aataattagg ggcttccagc cccttctata accattcatg gaacagggac
      481 aaccgaaatc actttaaacc ctgaaacagc cgttactcca ttagatggag aattaaatct
      541 cacagtatct tttcttactg catcatgaat ttcatctttt gttggttcat gctctgaatg
      601 gaataaaacc tcttccattc taatttgatc accgtctcta tgctcataag taatcttgta
      661 gctaacagtc atatcaacct catgcagggg aattgtgaga ttattataga caatagaaac
      721 cataaagccc cgttggtgat acagcaacaa gcgggagcgt agcgacttca tcagattgtc
      781 agaaagatca caccttatca ggataaccgt gtacccgttg ggagcgtggc tgaaacgctg
      841 atagagcttg catatggttt gcaaggacca atatcaccat aaacctgttt acgcagccac
      901 aaggccgcac atcgggctta aatgcgtgtg ttcagtctgt cgatgacatt acaatgccag
      961 gatgggcttt atcttcacgt tgtgctatcg cggtgtgaga ggtgcaactg ccgccaacgt
     1021 taggttatac tgtcatctca ccgtgtaact taacgtgaca gttactaagg gcggatcaca
     1081 atggtagaac tcctaagcaa attaaatagg taactgttta agttgctgac agagaagact
     1141 tttttagtct tctccacctg gggagattct ggagcgggcg aagggaatcg aaccctcgta
     1201 tagagcttgg gaagctctcg ttctaccatt gaactacgcc cgcttcgaga tgcgtaaggc
     1261 attataaacc ttacgctctc cttagcaagt gccgcgctgc tgactgctga ttaattcgcc
     1321 atcagcattt tggcttgctg ccctgcggcg gcaagtaacg tagcggatca attgccgttg
     1381 cacggtaacg aatctggaaa tgcaggcgaa cagatgccgc atccgtgctc cccatagtgg
     1441 cgattttttg cccagtcttc acgctttgcc cattatttac cagcatcgtg tcattatggg
     1501 cgtaagccgt aatgtaatct tcactgtgtt taatcatgat gagattaccg tagccacgca
     1561 gctggttgcc cacatacacc acctttcctg cacccgcggc gtaaataggt gtaccccgtg
     1621 gagctgagat atcaatccct ttattgccgc catctgctgt cgaatacggc atgataactt
     1681 tccctgtcgt tggccataac caacaacgtt gccctactgg cggccaggaa gatttcggta
     1741 ccgctgatga cggtgtaacc gatgcggttt tggtcgttga tttggcggtt gatttacgtg
     1801 tactactgct acttttcgcc ccacccagtt ttagtttctg accaacttca atggtgtaag
     1861 ggggggaaat gccgttcagt cgcgccagtt cttttacgct ggttcccgtg gtgcgcgaaa
     1921 tacgatatag cgtatccccc cgtttcacgg tgtaaacgga gccggaatat gttcctgtat
     1981 cggatgattt gctacccgaa cagcccgcca aaagcagtcc aaccgataac aacatcacga
     2041 tacccagaga ttttttattc aggcgtcccg cactcaaaat ctttcctcgc ttaaaaaaca
     2101 agacgcctta tcatagcagc cagcctcttg atacccaatc tcttgcttct ggaaaaacgt
     2161 ggtaatagag aaagaggtta atttcatttg gcaaagggaa gtatcatcag caaaatattc
     2221 gctgtagagg tatcgaactt cctgactttt taccctattt
//
LOCUS       DAZPEB010000321         2246 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZPEB010000321 DAZPEB010000000
VERSION     DAZPEB010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.327"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(111..449)
                     /locus_tag="ACHURJ_004169"
                     /pseudo
     CDS             complement(111..449)
                     /locus_tag="ACHURJ_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
     gene            complement(565..1116)
                     /gene="maa"
                     /locus_tag="ACHURJ_004170"
     CDS             complement(565..1116)
                     /gene="maa"
                     /locus_tag="ACHURJ_004170"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFR1282869.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKGVPDNVVVGGNPARIIKKL"
     gene            complement(1288..1506)
                     /locus_tag="ACHURJ_004171"
     CDS             complement(1288..1506)
                     /locus_tag="ACHURJ_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFR1282870.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(1532..1906)
                     /gene="tomB"
                     /locus_tag="ACHURJ_004172"
     CDS             complement(1532..1906)
                     /gene="tomB"
                     /locus_tag="ACHURJ_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFR1282871.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNYGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
BASE COUNT          660 a          492 c          490 g          604 t
ORIGIN      
        1 gtcactctcg tctggggaat atctccccgg cagccttcag ggaaaaatat catcagatgg
       61 ctgcttaaaa aagaacaaat ggtagtgtcc gctattgcca gtacacctca cagcgcggca
      121 ggtacaaagc gcgtgttgta ccattcgccg ttatagcaaa cgcggaaatc cagcacgtcg
      181 atatcttcat agcggtagcg tgggtagaca tcgaaaaaga agaaaccatt aagcaggata
      241 aataatacaa ccagtagcca gacagagcct gacagcgttt cggactgcaa catcaccgcc
      301 agggtggcaa aaaaagcaac gtacataccg ataaacagcc ccggatgttt acggataaaa
      361 ctgatactaa agcggggttt gttgtcgcgt ttttcgcggg tattcagaga ctcaatcgtt
      421 tcggtcagca ggcgttgtat ttcggtcatc gtttgccttc gtggtatctg caactttcca
      481 gggaacaccc tattttaacg tgggaatagt agaggggaag taacagatct accacaattt
      541 tgcataacag ttgcgaaaaa ccgattacaa ttttttaatt attctggctg gattaccgcc
      601 cacgacaacg ttgtccggga caccttttgt gacaactgca cctgaagcta ccacgacgtt
      661 atcaccaatg gtcacaccag ggttaatgac cgcgcgtccg ccaatccaga cgttattacc
      721 gatggtgacg ggtttcccca gttcagcacc gctattacgt gctacagggt cgatgggatg
      781 tgttgccgtg tagatatgaa cgcctggtgc caacatacag ttatcaccga tgcgaatagg
      841 gcagacatca agcatcacgc aatcgaagtt ggcgaaaaaa ttattgccga gaaaaatgtt
      901 atagccataa tcacagcgaa acgttggctc aatataagcc tctgtcacct gaccgaataa
      961 atcagcgaga atttgctggc gtaatgtgtg ctcttccgcc agggaatgat tgtatcggtg
     1021 aataagctga cgagcgcgca ggcgatcgcg agataacgtc tcatctgccg agcgatacaa
     1081 ctcaccagca atcatctttt ctttttctgt gctcattact gacctccggt tgctgaaaat
     1141 gcggcaacac agtaagacga aaagtcagtg aacagaacgt tcccattaca tctgtgtgat
     1201 aaagatcaca tagggtatgt cttcatggtg aaaaagtata aaattcttaa taaacagccg
     1261 gttatagctc cgaaagcgaa ttatttatta gcgaataaat ttccatactg aggaagggat
     1321 cttgtcgtat agtttattca tggtcaattc ggcgaggcgg tgatctgcgg ctgagtaaaa
     1381 taccgccagt tcattatctg ataattcgta tttatttttc tcgataacac gctccagcgt
     1441 gtcaattgtc tggcaacgac gtaaacgcat taaataatcg gttttcgtta aaggtttttc
     1501 ggacatactt ctacccatgg ttgtaataat tctaacaaga taaactcgca ggattctctt
     1561 tcgtcgcatt gacaaaacaa cggaatagtc gattacctga cttccgccat ttctgaagat
     1621 cctgcatatt aataccataa ctactgaaca acataaaggt gtcatccaga tattcgtcga
     1681 tctgctcaat gagcttattg tcttcattat acttaatttt gtaattaagt gcgaaggtcg
     1741 caatatgctc aatcagttca ttcaactgga ggttgatcgc cgaggttggg tcgttaaccc
     1801 agccataatt gctttcttca aggtttgcaa ggcagtcatg atacagggtt tcacagagaa
     1861 acttaagctg tgcgatatca tgtcttttgg gtgagtattc atccataacg cgtccccttc
     1921 ttagcggttg aactaacgga cacctttcgg gatggaaaaa acttactgac ctggacttgc
     1981 cctctcgttt gttagcttaa ctataagcca ctctttgcag gtattcattg cattattacg
     2041 aaaaattaca attaggaagg aatcgggaca ggactcactt tttcatacta gcactaaccc
     2101 gcagcaggta aaagcagttt gctcgaactg ttcataacat ttcaggatta tcgccagatg
     2161 aaattggtga cactaatacc aggattgctc tgaatatgac gtaataaccg aggaatgaat
     2221 aaagaattac cgcaaataat taagaa
//
LOCUS       DAZPEB010000322         2158 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZPEB010000322 DAZPEB010000000
VERSION     DAZPEB010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.328"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..374
                     /gene="tnpA"
                     /locus_tag="ACHURJ_004173"
     CDS             <1..374
                     /gene="tnpA"
                     /locus_tag="ACHURJ_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HFR1282872.1"
                     /translation="RPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQY
                     RKGGLLPSGKNMPALLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRL
                     KGKLTPALLQILIREIKGSSH"
     gene            374..721
                     /gene="tnpB"
                     /locus_tag="ACHURJ_004174"
     CDS             374..721
                     /gene="tnpB"
                     /locus_tag="ACHURJ_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR1282873.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            741..1895
                     /locus_tag="ACHURJ_004175"
                     /pseudo
     CDS             741..1895
                     /locus_tag="ACHURJ_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            1899..2058
                     /locus_tag="ACHURJ_004176"
                     /pseudo
     CDS             1899..2058
                     /locus_tag="ACHURJ_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          515 a          575 c          604 g          464 t
ORIGIN      
        1 ggcggcctaa ttttccttat gaatttaaaa tcgccttagt ggagcagtca ctgcagcccg
       61 gagcctgtgt ggcgcagatc gcccgtgaaa acggaatcaa cgataacctg ctcttcaact
      121 ggcgccatca ataccggaaa ggtggcctgc tgccttccgg aaaaaatatg cctgcactgc
      181 ttcccgtgac gttaacgccg gagccggata ataaaatccc ggctccggca caggaaccag
      241 agcagataaa tacaccgtcc gacagtctgt gctgtgagct ggttctgccg gccggaactc
      301 tcaggcttaa aggtaaactg acgccggcgt tattacagat acttatccgc gaaataaaag
      361 ggagcagcca ctgatgatat ctttccctgc aggttcgcgt atctggctgg ttgcaggtat
      421 caccgatatg cgaaatggct ttaacggcct ggcctcaaaa gttcagaacg tcctgaagga
      481 tgacccgttc tccgggcatc tgttcatctt ccgcggacgc cggggtgacc agataaaagt
      541 gttgtgggct gacagtgacg gactgtgcct ctttaccaga cgcctggagc ggggccgctt
      601 cgtctggccg gtcacccgtg acggcaaggt gcaccttact ccggcccagt tatccatgct
      661 tcttgagggc atcgactgga aacacccgaa acgaacggaa cgcgctggca tccgcatata
      721 acccgttgta aagtgaggat atggacacct cacttgctca tgagaatgcc cgcctgcggg
      781 cactgttgca gacgcaacag gacaccatcc gccagatggc gaaatacaac cgcctgctct
      841 cacagcgggt ggcggcttat gcttccgaaa tcaaccggct gaaggcgctg gttgcgaaac
      901 tgcagcgcat gcagttcggt taaagctcag aaaaacttcg cgcaaaaacc gaacggcaga
      961 tactggaagc acaggagcga atcagcgcac ttcaggaaga aatggcggaa acgctgggtg
     1021 agcaatatga cccggtactg ccatctcccc tgcgccagtc ttcagcccgt aaaccgttac
     1081 ctgcctcact tccccgtgaa acccgggtca tccgtccgga agaggaatgc tgcccggcct
     1141 gtggtggcga actcagttct ctgggatgtg atgtgtcaga gcagctggag cttatcagca
     1201 gcgcctttaa ggttatcgaa acacaacgtc cgaaactggc ctgttgccgg tgtgaccata
     1261 tcgtgcaggc accggtgcct tcaaaaccca ttgcacgcag ttatgccgga gcggggcttc
     1321 tggcccatgt tgtcgctgga aaatatgcag gctatctgcc gttataccgc cagtcagaaa
     1381 tataacgtcg tcagggcgtg gagctgagcc gtgccacact gggtcgctgg accggtgccg
     1441 ttgctgaact gctggagccg ctgtatgacg tcctgcgcca gtatgtgctg atgcccggta
     1501 aagttcatgc ggatgatatc cccgttccgg tccaggagcc gggcagtggt aaaacaagaa
     1561 ctgcccggct gtgggtctat gtccgtgatg accgtaacgc cggttcggaa atgcctccgg
     1621 cggtctggtt cgcgtactca ccggaccgga aaggtatcca tccacagaat catctggccg
     1681 gttacagcgg agtgcttcag gccgatgctt acggtggtta ccgggcgttg tacgaatccg
     1741 gcagaataac ggaagccctg ctgcgtatcg gtgaactgta tgccatcgag gcagaagtcc
     1801 ggggatgttc agcagaacag cgtctggcgg caagaaaagc cagagccgcg ccactgatgc
     1861 agtcactgta tgactggata cagcaacaga tgaaaattca ttcgctgaaa atggaatgtc
     1921 tccacggaga acactattat ccatcgggaa atagtgcggg caacagtgtt tagttatatc
     1981 gagtgcgatt acaatcgctg gcgacggcac cgttggtgtg gtggtctcag tccggaacaa
     2041 tttgaaaacc agagcctcac ttacgactgt gtccatatta tgtgggtagg atcaataaag
     2101 acgttttata tgtgtctgct tgcagccaga aaatttatac cctgcaggta tcataaac
//
LOCUS       DAZPEB010000323         2148 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZPEB010000323 DAZPEB010000000
VERSION     DAZPEB010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.329"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(1..121)
                     /locus_tag="ACHURJ_004177"
                     /pseudo
     CDS             complement(1..121)
                     /locus_tag="ACHURJ_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            398..1273
                     /locus_tag="ACHURJ_004178"
     CDS             398..1273
                     /locus_tag="ACHURJ_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HFR1282874.1"
                     /translation="MRKFILISMITLASVSLGACRQNATIKQDAPGQKAFLIDGQIYD
                     LHSGKIISSSELLADLATARHLIIGEKHDNAEHHQIELWLIQNLLIQRPQGSVLLEML
                     TSDQQPRVNQVKCWLKDNPVVRDSRVQELLNWQKGWSWEMYGDIVMQLLRGPYPLLNA
                     NIGREQMLALYKKNEFPKGKKSTAPVVQEALRETIIAMHEGNLESQQLTSMLSVQQQR
                     DRYMARQLLSAPVPSLLIAGGYHASKNMGVPLHMEDLATGTHPVVLMLAEKGMNITVD
                     HADYVWFVAPDTTKR"
     gene            1281..2057
                     /locus_tag="ACHURJ_004179"
     CDS             1281..2057
                     /locus_tag="ACHURJ_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HFR1282875.1"
                     /translation="MMNILHFPQSVKWSSWFICSLLLHGLIFLTFIWRFSEVQPAMSP
                     APAIMLQWAEIIEAPSSPLSLPVGIAQQESAVTEEKQQTEDRQQRPVTEDSDATIEIT
                     RKKKSSDGEKKKTRPPRKIKAQTSDSNPTAVSSNAAPQALVESSRIAAPFNSDSTKRD
                     NSEASWESRVKGHLNRYKRYPGDARKRARTGTAVVTFTVNTEGTIVSSFLEISSGTLS
                     LDREAIAVLERAQPLPKPPPEILEGGLFKVKMPITFKLKE"
BASE COUNT          606 a          498 c          491 g          553 t
ORIGIN      
        1 tcaggctcgc ccctccgttg taatgtgcca gccgggaggc cgctatcggc ggcttcttca
       61 gataacgacc caggtagcgc gccgtattcc tcccgccggc cattttcttc gacatgtaca
      121 tcaggcgcgg aactcatcgg ggcgcgggaa tgaaatccca ttcagactcc ggatagattc
      181 aagcaagccc acttatcgtc aaaatcggtg ttgcaaaaac gggagtgacc atagatccgt
      241 tttccaagcg gaccccataa tatcggctgg taaacatcac tcgctagtgc attctttttg
      301 ttggtaatga taattattta catttattct ttgatgtttt atcataatta atgttttatc
      361 aataaatata tggaaatata ttgtttcaga tgggaatatg agaaagttca tccttatttc
      421 tatgataacg ttagctagtg ttagtctggg agcctgcaga cagaatgcca ccataaagca
      481 ggatgcgcca gggcagaaag ctttcctgat tgacgggcag atttacgatt tgcacagtgg
      541 caaaattatt tctagcagcg agcttttggc ggatcttgct accgctcgac atcttattat
      601 tggtgaaaaa cacgataatg ccgagcatca tcagattgag ctttggctta ttcaaaatct
      661 gctcatccag cgaccacagg ggagtgtgtt gcttgagatg ttgacgtcag accaacagcc
      721 ccgagttaac caagttaaat gttggctgaa agataatcct gtggttcgag atagccgtgt
      781 tcaggaacta cttaactggc aaaaagggtg gtcctgggag atgtatggcg acatcgtcat
      841 gcagctatta cgtgggcctt acccgctgct caatgccaac attggccggg agcagatgtt
      901 ggcactgtac aaaaaaaacg aatttcctaa aggaaaaaaa tccactgcac ctgtggtaca
      961 agaagctctg cgtgaaacca tcatagctat gcatgaaggc aaccttgaaa gccagcaact
     1021 gacgtcaatg ctctctgttc agcagcagcg ggatcgctac atggcccgcc agcttctgag
     1081 tgccccggta ccgtcgttgt tgattgcagg tggttatcac gcgtcaaaaa acatgggcgt
     1141 accgttacac atggaagatc ttgccaccgg cacccatcca gtggtgttga tgcttgcaga
     1201 aaaaggaatg aacatcacag tagatcatgc tgactacgtg tggtttgtag cgccagatac
     1261 aacaaaaagg taaaccatgg atgatgaata ttctccactt cccacagtcg gtaaaatgga
     1321 gcagctggtt tatctgttct ctactgcttc atggactgat tttccttacg tttatctggc
     1381 gtttttccga agttcagccc gctatgtctc ctgcaccggc gatcatgctg caatgggcag
     1441 aaattataga ggctccttct tctccacttt cgcttccggt gggaatcgct caacaggagt
     1501 ccgcagtgac agaagaaaag caacaaactg aggacagaca acaacgtcct gtcactgaag
     1561 acagtgatgc aaccattgaa ataactagga agaaaaagtc atccgatgga gaaaagaaaa
     1621 agacacgtcc acctcgtaag ataaaggctc aaaccagcga cagcaacccc acagccgttt
     1681 ccagtaatgc tgcacctcag gctctggtcg aatcgtcccg tattgctgcg ccttttaaca
     1741 gtgactcaac aaagcgagat aattctgagg catcttggga aagcagagta aaaggccacc
     1801 ttaaccgtta taaacgttac cctggcgatg ccagaaaacg tgcacgaacc ggcacagccg
     1861 ttgtgacttt taccgtaaac acggaaggca ctattgtcag tagtttcttg gaaatctcgt
     1921 caggaaccct ttctcttgac cgcgaagcta ttgctgtttt agaacgcgct caaccacttc
     1981 ccaaaccacc gcctgaaata ttggagggag gattatttaa agtaaaaatg ccgattacct
     2041 ttaagttgaa ggagtaaagg gataattggc cgttcaagtt catttaactg aaatttaatt
     2101 ttcattttta tgctgaacat tttatcaaac ttttccactg tcgaaaaa
//
LOCUS       DAZPEB010000324         2113 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZPEB010000324 DAZPEB010000000
VERSION     DAZPEB010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.330"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..1214
                     /locus_tag="ACHURJ_004180"
     CDS             <1..1214
                     /locus_tag="ACHURJ_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR1282876.1"
                     /translation="LAMTATVGSLSLAIGVLAGPLAKLRLGFSLLTGSMNAVRVLPAL
                     WGMVTGSVSLLGGAIGALFSPVGLIVAALAGAAVLIWKYWDPIRAFFAGVFSGIMERL
                     TPLRETFERFGPVFDAIGSGISQVFNWFKSLLSPMESSKETLDKCTSAGEIFGNVLGG
                     ALQLVLTPAKMLLDTLAWILEKLGVLPDEAERARKKIEDAQRAAILQDKVALLQGDLA
                     KINPPKPVENGNGTGGDNPKDNKPLIDSNTGTLRRLSKIADNTGKLVEETKKRIGPGD
                     IVFKNLPRALAVRGEWQERKIAQVSKPAPAINITPVVPAPLPPALVPVVAASSRPVAE
                     AIRSPVASVPVTSRNREPVAFGFGGEIHVHLHNVVTQNPRELAKLVGEMVRAEMERRA
                     RAGRGCFYDKD"
     gene            1227..1715
                     /locus_tag="ACHURJ_004181"
     CDS             1227..1715
                     /locus_tag="ACHURJ_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1282877.1"
                     /translation="MMMIYGMFVFELRTLPHQQLQQNKSWRHVKNERVNRSASWQYIG
                     AGDDRIVLSGVLYPEITGGEVSLSLLTTQAYTGRPWPLIDGVGQIYGMYVLTETNTTR
                     SEFDRYGKAKKIEFSLTLERCDEDLRERLQSSSFSDMLSGFKDKVTSSLNSAASSVKG
                     LF"
     gene            complement(1744..>2113)
                     /locus_tag="ACHURJ_004182"
     CDS             complement(1744..>2113)
                     /locus_tag="ACHURJ_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR1282878.1"
                     /translation="GELRERGINFRSLTDSIDTSSPMGRFFFHVMGALAEMERELIVE
                     RTLAGLAAARARGRTGGRRPKLTKEQHEQIARLIKNGHDRKQLAIIYGIGTSTIYRYH
                     PVGDIQTEETTGQTQENENR"
BASE COUNT          458 a          512 c          594 g          549 t
ORIGIN      
        1 tgctggcaat gactgcaacg gttggctcgt tgtcgctggc tattggtgtg cttgctggcc
       61 cgctggcaaa actgcgtctt ggtttttccc tcctgaccgg atcaatgaat gctgtcaggg
      121 ttctgccagc actatgggga atggtgacgg gttccgtttc tttactggga ggcgctatcg
      181 gggcgttgtt cagtccggtt ggtcttatcg tggctgcgct tgccggagct gccgttctta
      241 tctggaaata ctgggatccc atcagggcat tttttgccgg ggtgttcagc gggattatgg
      301 aaaggctgac cccgttgcgc gaaacctttg aacggtttgg tcctgttttt gacgcaatcg
      361 ggagtgggat cagccaggtg tttaactggt ttaaatcgct gctgtcaccg atggagtcca
      421 gcaaggaaac tctggataaa tgtaccagtg ctggcgagat attcggtaac gttcttggcg
      481 gtgcgttaca acttgttctg acacctgcaa aaatgttgct ggatacgctg gcgtggatac
      541 ttgaaaagct tggtgtgctt ccggatgaag cggaaagggc gcgcaagaaa atcgaagacg
      601 cacagcgtgc ggccattctt caggacaagg ttgccttgct tcagggggat ctggcgaaaa
      661 tcaatccgcc gaagcctgtg gaaaatggca atggcaccgg aggtgataac cccaaagaca
      721 ataaaccgct catagacagc aataccggta cgctgcgcag actcagcaaa attgctgata
      781 acacaggtaa gctggttgag gagacgaaaa aacgcattgg ccccggcgat attgtcttta
      841 aaaacctgcc ccgagcactt gctgttcgtg gggagtggca ggagcggaag attgcgcagg
      901 tcagtaagcc tgcccccgca attaatatca cacccgtggt tccggctccg ctgcctccgg
      961 cgctggtccc tgttgttgcg gccagctccc gcccggtggc ggaggctata cgatctccag
     1021 tggcatcagt tcctgtaact tcccgtaacc gggagcctgt tgccttcgga tttggtggtg
     1081 aaattcatgt tcatctgcat aacgttgtta cgcagaatcc ccgcgaactg gcgaaactgg
     1141 tcggtgaaat ggtcagggca gaaatggaac ggcgcgcccg tgccgggcgt ggctgttttt
     1201 acgataaaga ttgaggagtc atggccatga tgatgatcta cggcatgttt gtttttgagc
     1261 tgcgcacgct gccgcatcag cagttacagc aaaacaaaag ctggcggcat gtgaaaaatg
     1321 aacgcgttaa ccgttcagca agctggcagt atatcggtgc aggtgatgat cgcatcgttc
     1381 tttctggtgt gctttatcct gaaattacag gtggcgaagt gtcgctgtcg ctgctgacta
     1441 cgcaggcgta tacaggacga ccctggcctt tgattgatgg cgtcgggcag atttacggca
     1501 tgtatgtcct gactgaaacg aatacgaccc gctccgagtt tgatcgctac ggtaaggcga
     1561 aaaagataga attttcactg actcttgaac gctgtgatga ggatttgcgg gagcgcctgc
     1621 aatcctcatc gttcagtgat atgctgtccg gcttcaaaga taaggtgaca tcatctctta
     1681 acagcgcggc cagttcagtt aaagggctgt tctgatttaa cacaaaaacc gctaatggtc
     1741 agattagcgg ttttcatttt cctgagtctg cccggttgtt tcttcagtct gtatatcgcc
     1801 tacagggtga taacgataaa tcgtcgatgt gccaatgccg taaattattg ccagttgttt
     1861 tctgtcgtgc ccgtttttga ttagccttgc tatttgctca tgctgttctt ttgtcagctt
     1921 cggtcgacgt ccgcctgtgc gcccccttgc gcgcgctgcc gccagcccgg ccagtgtacg
     1981 ttcaacaatt aattcacgtt ccatttcagc cagggcaccc atcacgtgga agaaaaaacg
     2041 ccccattgga gaagatgtgt ctatgctgtc ggtcagactg cgaaaattaa tccctcgctc
     2101 ccgtagttcc ccg
//
LOCUS       DAZPEB010000325         2108 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZPEB010000325 DAZPEB010000000
VERSION     DAZPEB010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.331"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..1214
                     /locus_tag="ACHURJ_004183"
     CDS             <1..1214
                     /locus_tag="ACHURJ_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR1282879.1"
                     /translation="LAMTATIGSLSLVIGVLYGKLATLRLGFDILTRSMNVVRVLPAL
                     WGMVTGSVSLLGGAIGALFSPVGLIVAALAGAAVLIWKYWDPIRAFFAGVFSGIMERL
                     TPLRETFERFGPVFDAIGSGISQVFNWFKSLLSPMESSKETLDKCTSAGEIFGNVLGG
                     ALQLVLTPAKMLLDTLAWILEKLGVLPDEAERARKKIEDAQRAAILQDKVALLQGDLA
                     KINPPKPVENDNGTGGDKPKDNKPLTDSNTGTLRRLSKIADNTGKLVDETKKRIGPGD
                     IVFKNLPRALAVRGEWQERKIAQVSKPAPAINITPVVPAPLPPALVPVVAASSRPVAE
                     AIRSPVASVPATSRNWEPVASGFGGEIHVHLHNVVTQNPRELAKLVGEMVRAEMERRA
                     RAGRGSFYDKD"
     gene            1227..1715
                     /locus_tag="ACHURJ_004184"
     CDS             1227..1715
                     /locus_tag="ACHURJ_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1282880.1"
                     /translation="MMMIYGMFVFELRTLPHQQLQQNKSWRHVKNERVNRSASWQYIG
                     AGDDRIVLSGVLYPEITGGEVSLSLLTTQAYTGRPWPLIDGVGQIYGMYVLTGTNTTR
                     SEFDRYGKAKKIEFSLTLERCDEDLRERLQSSSFSDMLSGFKDKVTSSLNSAASSVKG
                     LF"
     gene            complement(1751..>2108)
                     /locus_tag="ACHURJ_004185"
     CDS             complement(1751..>2108)
                     /locus_tag="ACHURJ_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR1282881.1"
                     /translation="GELQERGINFRSLTDSIDTSSPMGRFFFHVMGALAEMERELIVE
                     RTLAGLAAARARGRTGGRRPKLTKEQHEQIARLIKNGHDRKQLAIIYGIGTSTIYRYH
                     PAGESSGTIEKSQKTK"
BASE COUNT          460 a          512 c          594 g          542 t
ORIGIN      
        1 tgctggcaat gactgcaacg attggctcgt tgtcgctggt tattggggtg ctttacggga
       61 agctggccac cctgcgtctt ggttttgaca ttcttacccg gtcaatgaat gtcgtcaggg
      121 tgttgcctgc gctgtgggga atggtgacgg gttccgtttc tttactggga ggcgctatcg
      181 gggcgttgtt cagtccggtt ggtcttatcg tggctgcgct tgccggagct gccgttctta
      241 tctggaaata ctgggatccc atcagggcat tttttgccgg ggtgttcagc gggattatgg
      301 aaaggctgac cccgttgcgc gaaacctttg aacggtttgg tcctgttttt gacgcaatcg
      361 ggagtgggat cagccaggtg tttaactggt ttaaatcgct gctgtcaccg atggagtcca
      421 gcaaggaaac gctggataaa tgtaccagtg ctggcgagat attcggtaac gttcttggcg
      481 gtgcgttaca acttgttctg acacctgcaa aaatgttgct ggatacgctg gcgtggatac
      541 ttgaaaagct cggtgtgctt ccggatgaag cggaaagggc gcgcaagaaa atcgaagacg
      601 cacagcgtgc ggccattctt caggacaagg ttgccttgct tcagggggac ctggcgaaaa
      661 tcaatccgcc gaagcctgtg gaaaatgaca atggcaccgg aggtgataaa cccaaagaca
      721 acaaaccgct cacagacagc aataccggta cgctacgcag actcagcaaa attgctgata
      781 acacaggtaa gctggttgat gagacgaaaa aacgtattgg ccccggcgat attgtcttta
      841 agaacctgcc ccgcgcactt gccgttcgtg gggagtggca ggagcggaag attgcgcagg
      901 tcagtaagcc tgcccccgca attaatatca cacctgtggt tccggctccg ctgcctccgg
      961 cgctggtccc tgttgttgcg gccagctccc gcccggtggc agaggccata cgatcgccag
     1021 tggcatcagt tcctgcaact tcccgtaact gggagcctgt tgcctccgga tttggcggtg
     1081 aaattcatgt tcatctgcat aacgttgtta cacagaatcc ccgcgaactg gcgaaactgg
     1141 tcggtgaaat ggtcagggca gaaatggaac ggcgcgcccg tgccgggcgt ggcagttttt
     1201 acgataaaga ttgaggagtc atggccatga tgatgatcta cggcatgttt gtttttgagc
     1261 tgcgcacgct gccgcatcag cagttacagc aaaacaaaag ctggcggcat gtgaaaaatg
     1321 aacgcgttaa ccgttcagca agctggcagt atatcggtgc aggtgatgat cgcatcgttc
     1381 tttctggtgt gctttatcct gaaattacag gtggcgaagt gtcgctgtcg ctgctgacca
     1441 cgcaggcgta tacaggacga ccctggcctt tgattgatgg cgtcgggcag atttacggca
     1501 tgtatgtcct gaccggaacg aatacgaccc gttccgagtt tgatcgctac ggtaaggcga
     1561 aaaagataga attttcactg acccttgaac gctgtgatga ggatttgcgg gagcgcctgc
     1621 aatcctcatc gttcagcgat atgctgtccg gcttcaaaga taaggtgaca tcatctctta
     1681 acagcgcggc cagttcagtt aaagggctgt tctgatttaa cacaaaaacc gctaatggtc
     1741 agattagcgg ttattttgtt ttctgactct tctctattgt tccgcttgat tctcctgcgg
     1801 ggtggtaacg ataaatcgtc gatgtaccaa tgccgtaaat tattgccagt tgttttctgt
     1861 cgtgaccgtt tttaatcagc cttgctattt gctcatgctg ttcttttgtc agcttcgggc
     1921 gacgtccgcc tgtgcgcccc cgtgcgcgcg ctgctgccag tccggccagt gtacgttcaa
     1981 caattaattc acgttccatt tcagccaggg cacccatcac gtggaagaaa aaacgcccca
     2041 ttggagaaga tgtatctatg ctgtcggtca gactgcgaaa attaatccct cgctcctgta
     2101 gttccccg
//
LOCUS       DAZPEB010000326         2094 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZPEB010000326 DAZPEB010000000
VERSION     DAZPEB010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.332"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..335)
                     /locus_tag="ACHURJ_004186"
     CDS             complement(<1..335)
                     /locus_tag="ACHURJ_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol phosphate phosphatase SopB"
                     /protein_id="HFR1282882.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGNQKGVSVISYQRVKNGER
                     NKGVEALNRLYLQNQTSLTGKGLLFARDRAAVFYEAIKLAGGDTSKIKAMMEQLDTYK
                     LGEVDKRNI"
     gene            649..>2094
                     /gene="icsB"
                     /locus_tag="ACHURJ_004187"
     CDS             649..>2094
                     /gene="icsB"
                     /locus_tag="ACHURJ_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HFR1282883.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPILVAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLANQKSLLLFLKTLASEKSAESAFAAYEAVKNSIQHSF
                     TGKDIKLMLNTAESFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISYNLFGLDE
                     TNMSKFICQKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NNVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDAVINACHSYLVKSAPSNMSRVLNELKTGATDKKEV
                     IIEKSIKTIDYYNSLKSPDLGTKLYIHDLLQV"
BASE COUNT          746 a          405 c          316 g          627 t
ORIGIN      
        1 ttaatatttc ttttatcaac ttcaccaagt ttataggtat ctaattgttc catcatggct
       61 ttaatttttg acgtatcacc acctgccaac tttattgcct cgtaaaaaac tgcagctctg
      121 tcacgcgcaa acaaaaggcc tttccctgtt aagcttgttt ggttctgtaa atataatcga
      181 ttaagcgctt cgaccccctt atttctctct ccatttttaa ctctttgata tgaaatcacg
      241 cttacacctt tttggtttcc ggtttcttct gcgcctttat aggaatctcc gctttgaaat
      301 gaaacctgat gcaatcccaa attagttatg tgcatttaat tatcctcagt cataaatggg
      361 gcattgattg cagttttgta cctcgtttta caacaaaaaa gatacttatt caacagggtt
      421 ctctcttttt ctataacact ccgagcctga tatatcagac actttaactt gtgggatttc
      481 atgatgaaac cagcactaca tatgctcacg aggtacatat tatgtacagc aaaagaaaat
      541 ttgattatgt taacaggctc aagtttctaa atttattaat tgaatatatt atatatcaaa
      601 tacattttac atccccacaa tcaccacgta atggagagtt aataaagtat gattctcaaa
      661 attagcaatt tcattgacgc aagcaataca aaagggccta tacgcgttga agatacagag
      721 catggaccta tattggtagc acagaaattc aacctaaagg atctcttttt cagaacatta
      781 agcactatta atgcaaagat aaattcacag atattaaatg aacaactaaa aaattatcga
      841 ctagcgaatc aaaaatcctt attgcttttc ctcaagacat tagctagcga aaaatctgca
      901 gaaagtgcgt ttgccgccta tgaagcagtc aaaaattcaa ttcaacattc ttttacaggt
      961 aaagacatca aattaatgtt gaataccgca gagagttttc atggcatcgg tacagccaaa
     1021 aatcttgaaa gacatttagt gtttcgttgc tggggaaata gaggcataac ccatttaggg
     1081 cataccagta tcagtataaa aaacaattta ctacaggaac caactcatac ctatctcagc
     1141 tggtatcccg gcgggaatgt tacaaaagat acagaaataa actacctttt tgaaaaacgc
     1201 tcaggttaca gtgtcgatac ctataaacaa gacaaactaa atatgatttc agaccaaaca
     1261 gccgaaagac ttgatgcagg ccaagaggtt agaaacttat taaattcaaa acaagatcaa
     1321 aataacaaca aaaaaatatt ttttcccaga gctaatcaaa aaaaagatcc ctatggttat
     1381 tggggggtca gtgctgataa agtatacatt ccattatcag gtgataataa aacaaaggat
     1441 ggtaaaatca gctataacct ttttggcctt gatgaaacaa acatgtcaaa atttatatgc
     1501 caaaagaaag cagatgcttt cagacaattg gcaaattata aattaatcag taaatctgaa
     1561 aactgtgctg gtatggcact taatgttctt aaagcaggaa attctgaaat atactttcca
     1621 ctccctgacg ttaaattggt tgctacacct aacaatgtat acgcgtatgc aaataaagtt
     1681 aggcaacgca ttgaaagtct caatcagtct tataacgaga taatgaagta tattgaatct
     1741 gattttgatc tttccagatt gactcaatta cgacgtagct atcttaaaag tttcaataaa
     1801 attaacctta tccacacccc aaaaacattc aagcctttat caatttcact ttacaaacat
     1861 cctactgaaa atgtatcttc agaagacttt gatgcggtca tcaacgcttg ccactcatac
     1921 ttagtaaaat cagcacccag caatatgtct cgtgtattaa acgaattaaa aaccggagca
     1981 acagataaaa aagaagtaat tatcgaaaag tcaataaaaa ctattgatta ttacaattct
     2041 cttaaatcgc cagatcttgg aaccaaactt tacattcacg acttattaca ggtc
//
LOCUS       DAZPEB010000327         2078 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZPEB010000327 DAZPEB010000000
VERSION     DAZPEB010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.333"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(62..934)
                     /locus_tag="ACHURJ_004188"
                     /pseudo
     CDS             complement(62..934)
                     /locus_tag="ACHURJ_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
     gene            complement(948..1088)
                     /gene="yqcG"
                     /locus_tag="ACHURJ_004189"
     CDS             complement(948..1088)
                     /gene="yqcG"
                     /locus_tag="ACHURJ_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope stress response protein YqcG"
                     /protein_id="HFR1282884.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDNESKITDAAVNLFI
                     QI"
     gene            1227..1898
                     /gene="queE"
                     /locus_tag="ACHURJ_004190"
     CDS             1227..1898
                     /gene="queE"
                     /locus_tag="ACHURJ_004190"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFR1282885.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          610 a          423 c          454 g          591 t
ORIGIN      
        1 ccagtcagtt gcttaactga ctggcattac agcctccggg atggcatttt ttatggttga
       61 gttaccagcg gccggatgcg ccgccacctc cagaagaacc gccaccgccg ctaaatccgc
      121 cgccacttga gcctccaccg gaaccgctgt tgtctgaatg ataactttta cctgatgctc
      181 ttttctgaag tacacataaa caggtaaaga ccataaatat ggtgaaagta aaaaagaagg
      241 aaaatacagc aatggaaaaa attttatcgg agaacaggaa taaatctaaa agatagattg
      301 ctgaaataca gactccactt ttacatgcgc gacaaaaatt actgccttga tgaaaaaacc
      361 agaaagggaa gaacatcact gcaaataccc aaaaaatagc aaagtaataa tgatcactgg
      421 aagacgctga ttcactttct gaaggattag tcggatattg gtgttgtgaa gtgagttggt
      481 tattcaaagc gtttatagct agctctaatc cctgagcaaa tttttgttgt ttaaatgcgg
      541 gtatcatgtt gctacggatg atatctccag ccagagcatc ggttaccttt tcttccagcc
      601 cataacctac ctggatgcgg acagtacgat ctgaccaggc aacaatgatc agtatcccat
      661 cattacgttt ggcatctcct aaacgccaat tgtcaaaaac tcttgtagca tattgttcaa
      721 ttgtctcgtc tttggttgtg ggaacaatta atacagcaat ttgcacttgg gtattattga
      781 aaatagattc taacttgtgc gtcagttctt tcttttcttg tgagtttaag ctactcgtaa
      841 gatccgtaac ttgttactgt aattgtggga caatagttgg ttgggctgca acaaaggaac
      901 atacgaaagt gaacattagt atgaaatagc gcattataaa tttctcatta aatctggata
      961 aataaattga cggcggcatc ggtaatttta gactcgttat cattaaaaac taatacagaa
     1021 aaaataaata taattttggt gaatgttttg acatgattaa atccattttc tttattttct
     1081 tctgacataa tacgttactc cctttaatta ttaacgatat gtttcgcatt tttctttaaa
     1141 tctgcatgta acagaatgtt aataaggcat gattgttgcg gcaggatctg caataattaa
     1201 caattatcca cttcacagag aatgctatgc agtacccgat taacgagatg ttccagaccc
     1261 tgcaaggtga gggttacttt accggcgtcc ccgccatttt tattcgttta cagggatgcc
     1321 cggttggctg tgcctggtgc gacaccaaac acacctggga aaagcttgag gatcgggaag
     1381 tctccctttt cagcattctg gcgaagacca aagagagtga taagtggggg gcggcgagca
     1441 gtgaagattt gctggctgtc attggtcggc agggatatac cgcgcggcat gtggtgatta
     1501 cgggtggtga gccttgcatt catgatttgc tgccactgac tgacctgctc gaaaagaacg
     1561 gttttagctg ccagatcgaa accagtggaa ctcatgaagt acgctgcaca ccgaatacct
     1621 gggttaccgt atcgccaaag ctgaacatgc gcggcggcta tgacgtgttg tcacaggcac
     1681 tggagcgagc caacgaaatc aagcatccag tggggcgcgt acgcgatatt gaagcactcg
     1741 atgaactatt ggcaacgctg accgatgata aaccgcgagt cattgcactg cagccgatta
     1801 gccaaaagga tgatgccaca cgtttgtgca ttgaaacctg cattgcgcgt aattggcgtt
     1861 tgtcgatgca aacacataaa tatctaaata ttgcctgatt aaacatttat aagcgttata
     1921 aatgggtgga acctgtaaag acttctactc atttatattg tttgtcgcct ctgaaaactc
     1981 ctccatttta cccatccagg gctaatcatt agcattatct acaaattctg tggcattaat
     2041 ttttcgctgg agtgaaaatt attgcggtaa agtttggt
//
LOCUS       DAZPEB010000328         2069 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZPEB010000328 DAZPEB010000000
VERSION     DAZPEB010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.334"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..552
                     /locus_tag="ACHURJ_004191"
     CDS             <1..552
                     /locus_tag="ACHURJ_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFR1282886.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQTDLLKNTKETEIGGATGVGVRILNSQSGEVTLGTPVVITFNNTNSYQELNFKVRM
                     ESPGKDATPGNVYAQADYKIAYE"
     gene            612..>2069
                     /locus_tag="ACHURJ_004192"
     CDS             612..>2069
                     /locus_tag="ACHURJ_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR1282887.1"
                     /translation="MYKKLKLTTISELIKNIYCSLSVLIIGCASAYAVEFNKDLIEAE
                     DRENVNLSQFETDGQLPVGKYSLNAIINNKRTPIHLDLQWVLIDNQTAVCLTPEQLTL
                     LGFTDEIIEEAQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPP
                     ELWDHGIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFN
                     NGKSQANNLDFPRIYLFRPIPAINAKLTIGQYDTESSIFDSFHFSGVSLKSDENMLPP
                     DLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVPPGPFAITNLFNTLQGQLDVKVEEE
                     DGQVTQWQVASNSIPYLTRKGQIRYTTAMGKPTSVGGDSLQQPFFWTGEFSWGWLNNV
                     SLHGGSVLTNRDYQSLAAGVGFNLNSLGSLSFDVTRSDTQLHNQDKETGYSYRANYSK
                     RFESTGSQLTFAGYRFSDKNFVTMNEYINDTNHYTN"
BASE COUNT          625 a          439 c          422 g          583 t
ORIGIN      
        1 atgtctgctt ttaaaaagtc acttcttgtt gctggcgtag caatgattct ttccaataac
       61 gtatttgctg acgaaggtca cggcattgtt aaatttaaag gtgaagttat ctctgctcca
      121 tgttcaatta aaccaggtga cgaagactta actgtaaatc tgggtgaagt agcagatacc
      181 gttctgaaaa gcgaccagaa atctttggct gagcctttca ctattcacct gcaagactgc
      241 atgttgagcc agggtggaac cacttattca aaagcgaagg ttactttcac cacagcgaat
      301 actatgacgg gtcagactga cctgctgaaa aacaccaaag aaactgaaat cggtggtgcc
      361 acaggcgttg gcgtacgtat tctgaatagc cagagcggcg aagtgacttt aggtactccg
      421 gtagtaatta cattcaacaa tactaacagc tatcaggaac tgaacttcaa agttcgtatg
      481 gaatccccgg gcaaagatgc taccccgggc aacgtttacg ctcaggctga ctataagatt
      541 gcttacgagt aatcacatta ggccgggttc gcccggccac atcattaacg gattaatgat
      601 aagtggaaca gatgtataaa aaattaaaat taaccacaat aagcgaattg attaaaaata
      661 tttattgttc attatccgtt ctcatcattg gttgtgcgtc agcttatgcc gttgaattca
      721 acaaagattt aatcgaagcc gaagatcgtg aaaacgttaa cctttcccaa tttgaaactg
      781 atggccaatt acccgtaggt aaatactcat taaacgctat tattaataat aaaaggacgc
      841 caatccatct ggacctgcaa tgggtattaa ttgataacca aactgcagtt tgtctgactc
      901 cagagcaatt aacattatta ggctttactg atgagattat tgaagaggct cagcaaaacc
      961 tgatcgatgg ttgttaccct atcgaaaaag aaaaacaaat tacaacttat ctcgataaag
     1021 ggaaaatgca attatccata tctgcacctc aggcatggtt aaaatacaaa gatgcaaact
     1081 ggacgcctcc tgaactttgg gatcatggta ttgctggggc atttcttgac tacaatttat
     1141 atgcctctca ttatgcacca catcagggcg ataattcaca aaatataagt tcctatgggc
     1201 aggctggggt taatcttggg gcctggcgcc tgcgtactga ttaccagtac gatcagtcat
     1261 ttaacaatgg caaaagccag gcgaacaacc tggattttcc gcgtatttat ttgtttcgcc
     1321 caatcccagc aattaatgca aaactaacta taggtcaata cgatactgaa tcctctattt
     1381 tcgactcttt ccatttttct ggcgtttcgt tgaaaagcga tgagaatatg ttgccgcctg
     1441 atctacgtgg ttacgcaccg caaatcacgg gtgttgcaca aacgaatgca aaggtcactg
     1501 tctcacagaa caaccgtatt atttatcaag aaaatgttcc tcctggccca tttgctatta
     1561 ctaatttatt caatacatta caggggcaac tggacgtcaa ggttgaagaa gaggacgggc
     1621 aagtcacgca atggcaagtt gcatctaata gtattcctta tctgacgcgt aaagggcaga
     1681 ttcgctacac cactgctatg ggtaaaccga ccagcgttgg tggtgattcc ttacaacaac
     1741 ccttcttctg gactggtgaa ttctcatggg gttggctgaa caatgtatcc ctgcatggtg
     1801 gttcagtttt aacaaaccgt gattatcaat ctctggctgc cggcgttggc tttaacctta
     1861 actcattggg ttcattatct tttgatgtca cacgatctga tactcagttg cataatcagg
     1921 ataaagaaac gggttatagc taccgcgcta actattcaaa acgttttgaa tctaccggta
     1981 gccagctcac tttcgctggt taccgtttct ctgataaaaa ctttgtgaca atgaatgaat
     2041 atatcaatga cactaaccat tacacgaat
//
LOCUS       DAZPEB010000329         2062 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZPEB010000329 DAZPEB010000000
VERSION     DAZPEB010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.335"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..1291)
                     /locus_tag="ACHURJ_004193"
     CDS             complement(<1..1291)
                     /locus_tag="ACHURJ_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR-type extended signal peptide-containing
                     protein"
                     /protein_id="HFR1282888.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVT"
     gene            complement(1335..2018)
                     /locus_tag="ACHURJ_004194"
     CDS             complement(1335..2018)
                     /locus_tag="ACHURJ_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFR1282889.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IDLLEKQQAQLKSQLETIQKQQTGIISSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
BASE COUNT          509 a          513 c          414 g          626 t
ORIGIN      
        1 ctgtaacagt accgtcatca acatctactg cagcccctcc tccaagcctt tttgctaccg
       61 agtcgctaat ggcataaagc tgtgaaccat taatggcatc gtaactgtca gacttgatcg
      121 cgccattttt cacattaacg atcttgcgtt ttaatgaact attgccgaca gagacggtat
      181 tgtctaaatc cgcaatggaa tctgcgccca gcgcgacaga gttaataccg ttggcactgc
      241 tattactacc aagcgcaaga ctcttatcac cagttgcatt actggctgtg cctaaggcaa
      301 tggtgtttaa gcctctcgac tggctaccat tacccagagc gatggcatcc acgccatcag
      361 ctttgttgcc ctggcctatg gcgattgcat tatctgccag agcctggctg ccatttccta
      421 atgcgatagt attgacttta tcggcaaggc ttccgaaccc cattgccatg ctattagtcc
      481 cttttgcctt cgcattattg ccaatagcag ttgcattttc agcggcttcg gtctcagcac
      541 ctatcgcaat agcgttcgct gcgctggcaa gtgattgtct gccaaatgcg aggctgtcag
      601 taccgctagc tacactgcta cgccccaggg caattgcgtt ttcttcagat gcgacgctac
      661 ttgctcccag cgccattgaa tacgcttttg acgcattggc ggtgtcgcca agagcaatac
      721 tcataatctc agtagcttta gtggcatttc ccagggcaat ggcgtttgct ccttccgctt
      781 tggaagagtc ccccatagca atagacaatt tacccaatgc ttttgaataa cggccgaggg
      841 ccattgagta ttggcccaat gaatacgaag aggcacccag tgctatactt ctatcgcctt
      901 ctgatattgc actaacccca aaggccattg atgcaccacc aatcgcatgg gtttttgacc
      961 cgatggcaga gctatattgg ccttcagcta tagcgtcata acccacagca gtactggaac
     1021 cactggtgtt cataaaagta tttgctttcg cccctttgcc tatagcaacc cagccgtcgc
     1081 cagctgatcc actaccgtaa tcaacaccct gaccgttgtc attcccggca ttcgccaatg
     1141 ccccaaacga cgacaacatt ccacccgcaa ccagcgcaga aattaacagc ttactgcgcc
     1201 cagatttctt gccacggctt tttgccgttt cgctggtaac agtataattc cctgtcgcag
     1261 ggttccagat aactttaaat attttgttca ttaatgatgc tcgctattcc ttgttataag
     1321 tgtactaact gcatttattt tttgcttctt ttctgccgat tttcagcaga gggaagattg
     1381 tcgaactgaa tattttccat gttgatggct aacttcagat atcccaggtt attgggcgag
     1441 acacccttca gggtcaaatt aatgtcttgc cattgacctg gtaataattt caatgtggat
     1501 ttcattgatg tgttgaaaag taattgttgc caccgggcaa actctttggc atcgacaggt
     1561 ttttcaccac cccatgtcgc attaaaacca atatttgaga taggtgtact taataagttg
     1621 accagtgaaa tatgaataac actgccattc tctgtggcgt caatggataa cactcgaccc
     1681 gctaaagcca ccgaaccatt gttcaggatg aaatacttcg tttttgctgg gttaaactcg
     1741 gtaaaaataa aagtgttttg ttgatcttta acggatttaa ttacgtgagt taaagtcttt
     1801 gtactgctta ttatgccagt ttgttgtttt tgaatagttt ccagttgact ctttagctgt
     1861 gcttgttgtt tttcaagtag atcaatttta ttgttatgtt cttcttgttg tttcaataaa
     1921 ctactttctg gcttgccttc ttcaatgtag tcacatcccg ttaatgcgca gagaactgaa
     1981 acagtgacag cactgaaaaa aatcttcttc aagttcatgt aaaaatcctt atttcctccg
     2041 accagcgatc aatgccggta ga
//
LOCUS       DAZPEB010000330         1988 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZPEB010000330 DAZPEB010000000
VERSION     DAZPEB010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.336"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..339
                     /locus_tag="ACHURJ_004195"
     CDS             <1..339
                     /locus_tag="ACHURJ_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282890.1"
                     /translation="LVISIIIRIYGRIFSVSCFSDIPFVREHRIKLIMAGFLVYFWLF
                     SFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSFKDDSNRFLIFNTEQSGF
                     YEINDVYVRK"
     gene            573..701
                     /locus_tag="ACHURJ_004196"
                     /pseudo
     CDS             573..701
                     /locus_tag="ACHURJ_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_039289737.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            891..1295
                     /locus_tag="ACHURJ_004197"
     CDS             891..1295
                     /locus_tag="ACHURJ_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282891.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            1358..1988
                     /locus_tag="ACHURJ_004198"
                     /pseudo
     CDS             1358..1988
                     /locus_tag="ACHURJ_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          554 a          443 c          497 g          494 t
ORIGIN      
        1 ttggttattt caataataat tcgcatatat ggacggatct tttctgtatc atgtttttca
       61 gatatccctt ttgttagaga acaccgcatt aagttaatta tggctggttt tttggtttat
      121 ttttggcttt tttcctttct tgttggctgg tataaacctc aattaaaaaa agaatatcag
      181 atgctttgtt ataataatag ttggtattat gttcttgctc gttatgattc acggctcgtt
      241 ttgtctagtt catttaaaga tgacagtaac agattcttaa tatttaatac agaacagtcg
      301 ggattttatg aaatcaatga tgtttatgtc agaaagtaat aatcctctcc cacaaagtga
      361 attaaacgaa tatataatgc gttcgaagta tatgtattga gctgtcatac tgagaaacaa
      421 accttagggc agcacaatta aagccagaaa caactatgca agaaagttac taaataagtg
      481 ctcggaacag taaaacactc aggggggact cagtccagat tatgtaatca gataaattgc
      541 caaacatcac aaatcaccag caggactgag tgatgccgag cattgcacca ataccccgtg
      601 atgaacgatg cctgatgcag aaagctccct ataaaacaaa gaacaaaaac tacgaccggc
      661 gattcaccgt catactgatg ctgcatcgtg gtgacactgt ctgtcacttt catgttgact
      721 gaacaactct gctgcgaaga aggtgtaccg ccggcgggat ttgattgtcc ggcgtctagc
      781 ttccacttcc ctgatggaac tgaaagttgt tatggccggt cagcttgccc tgtccgcagg
      841 acatgaggtg ctgtgacgga cagcaccagt aacggaaagt gacaataaaa atgaatactg
      901 cgctgatagt tgcactaatg tgtatgtggt atgcggtacc cgctgctgca aaagagactt
      961 tactggctat gccacgtaat tcaacagaac attgctatgc ggagattaat gtgcacggac
     1021 catatggggt gtatttccga gtggtcccgc atccccccgg gggaaagagc tgggtcgaat
     1081 gtaacagtga ctactattac tctgataaac caccgggagt acagatactc ggaacccgtg
     1141 cagggtgtcg ggtatacgga atctgcggga ccacctcgac actgcatgtg gctggaagag
     1201 gggttgtgtg tattaaaaat atatgcagtc cacgtgggat gataattcac aggatacgga
     1261 aacgtcctgt agtggctgta agcgatgaga tgtgatctta tccagcaata gtggacacgc
     1321 ggctaagtga gtaaactctc agtcagaggt gactcacatg acaaaaacag tatcaaccag
     1381 caaaaaaaca cgtaaacagc attcgcctga attttgcagt gaagccctga agcttgctga
     1441 acgcatcggt gttgctgccg cagcccgtga actcagcctg tatgaatctc agctctatgc
     1501 gtggcgcagc aaactgcagc aacagatgac ctcgtctgag cgtgaaagtg aactggccgc
     1561 tgaaaatgcc cgcctgaagc ggcaactggc ggtgcaggca gaggaactgg ctatcctcca
     1621 aaaggccgcg acatactttg cgaagcgcct gaaatgaagt atgtctttat tgaaaaacat
     1681 caggctgagt tcagcatcaa ggcaatgtgc cgcgcgctcc gggtggcccg cagcggctgg
     1741 tatacgtggt gtcagcggcg gacagggata agccctcgtc agcagttccg tcagcactgc
     1801 gacagcgttg tcctcgcggc ggcctttacc cggtcaaaac agcgttacgg tgcaccacgc
     1861 ctgacggatg aactgcgtgc tcagggttac cactttaacg taaaaaccgt ggcggcaagc
     1921 ctgcgccgtc aggggctgag ggcaaaggcc tcccggaagt tcagcccggt cagttaccgc
     1981 gcacatgg
//
LOCUS       DAZPEB010000331         1969 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZPEB010000331 DAZPEB010000000
VERSION     DAZPEB010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.337"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            1..1571
                     /gene="ycgH"
                     /locus_tag="ACHURJ_004199"
                     /pseudo
     CDS             1..1571
                     /gene="ycgH"
                     /locus_tag="ACHURJ_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768545.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter YcgH"
     gene            complement(1628..1963)
                     /locus_tag="ACHURJ_004200"
     CDS             complement(1628..1963)
                     /locus_tag="ACHURJ_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFR1282892.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
BASE COUNT          546 a          437 c          489 g          497 t
ORIGIN      
        1 ttctggttaa taatactgga ggtgacttaa acataatttc gtccgactct attctaaatg
       61 gagcgactaa actcgttagc ggcacaacca cgctgaagct ttcagaaaat acaatctgga
      121 atatgaaaga tgactccgtt gttacccatc tgactaattc agacagtatt atcaatcttt
      181 cgtatgatga tggtcaaaca tttacccaag gaaaaacatt aaccgtaaaa ggtaattatg
      241 tcggtaataa tggtcagctt aatatccgca ccgtattagg tgatgataaa tcggctacgg
      301 acagacttat tgttgagggt aatacttcgg gttcaactac cgtctatgtg aaaaatgctg
      361 gaggaagcgg cgcggccacg ctaaacggga tcgaactcat aactgtgaat ggcgatgaat
      421 ctccagcaga agccttcaga caaggtgatg caaggattgc agctggagct ttcgaatatc
      481 aactaaaaca acaaggcaaa aactggtatt tgacaagtta tcagtcagtg aatgaggagg
      541 ataacagctc agagggaaat tcagaaccaa cagaaacgcc tacccccgtc ctgcgccccg
      601 aagctggaag ttacgtcgcc aacctggcag ccgctaacac tctttttgtt atgcgtctga
      661 acgaccgtgc gggtgaaatg cgctacatcg atcctgtaac tgaacaggag cgttcaagcc
      721 gactttggct acgtcaaatt ggcgggcata atgcctggcg tgacagcaac ggacagttga
      781 gaacgacctc gcatcgctac gtctcgcagt taggggccga tctgttaacc ggtggtttta
      841 ccgacagtga cagttggcgt ttgggagtga tggctggtta tgcccgcgac tacaactcaa
      901 ctcattccag cgtgtcggat tatcgttcga aagggagtgt cagaggctat tgcgcagggc
      961 tgtatgccac ttggtttgcc gatgacatca gtaaaaaagg cgcatacatt gacgcctggg
     1021 cgcaatatag ctggtttaaa aactcggtga aaggggatga attagcctat gaatcctata
     1081 gtgcgaaagg cgcaaccgtc tcgctggaag cgggttaggg ctttgccctg aataaatcct
     1141 ttggtctgga agcggcgaaa tacacgtgga tattccagcc acaggcacag gctatctgga
     1201 tgggcgtcga tcataatgcg cacaccgaag ccaatggctc acgtattgag aatgacgcaa
     1261 ataacaacat ccagacccga cttggtttcc gcacctttat tcgtactcag gagaaaaaca
     1321 gcggtccgca cggtgacgac tttgaacctt ttgttgaaat gaactggatc cataacagta
     1381 aagattttgc tgtctcaatg aacggtgtga aagtcgaaca agatggggcg agtaatttgg
     1441 gggaaattaa acttggcgta aatggcaacc tgaatccagc ggccagcgtc tgggggaatg
     1501 tgggcgtgca gctgggtgat aatggctaca atgacaccgc agtgatggtg ggcctgaaat
     1561 ataagttctg atcccgccgt tagctaaaaa accgcgtcgt attcatcgac gcggtacata
     1621 tgaaatatta ttttttgccg atagcacgca tggtgtcatc aattgccgtg atcaacagca
     1681 tttgcgggtc tttagcgcaa acctgattca gtttttctac cactttggcg ctcagttccg
     1741 gagattgctc aatttttaaa tcacggctgg caacgctggc attacccatt accgcaacaa
     1801 tttctgcaac ctgtgcgctg tcagtttttg ccatttcgtt ggcttctgcg caagtaatat
     1861 aggtttctga cggtaaaccg tttttaatat tgtagtcctg cgcccaggtc attggtgcga
     1921 aaacaaacag gcccgccagt aaagcaaatt ttttcatcat cattcctta
//
LOCUS       DAZPEB010000332         1962 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZPEB010000332 DAZPEB010000000
VERSION     DAZPEB010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.338"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            26..556
                     /locus_tag="ACHURJ_004201"
     CDS             26..556
                     /locus_tag="ACHURJ_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFR1282893.1"
                     /translation="MSNSLLPPSASSFMRCAEAVGTRITDIPVDLNTLWSPDTCPVHL
                     LPYLAWAFSVDRWDRNWPEEAKRQVIRDAWLIHRHKGTISALRRAIEPLGYLIRVSEW
                     WEFGGEPGTFTVEVGTLDSGVTEEMYLEMERLIADARPVSRHMTGLNIIQEIPGDIFA
                     AAATYDGEVITIYPDD"
     gene            559..>1962
                     /locus_tag="ACHURJ_004202"
     CDS             559..>1962
                     /locus_tag="ACHURJ_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFR1282894.1"
                     /translation="MSTTTRKFKTVITDTGAKKLAQAAAPDGKPVRLTHMAVGDGGGT
                     LPTPDSKQTRLVHEVWRHTVNRVILDATHQNRIIAELVIPPETGGFWIREIGVFDEHG
                     DLIAVGNTAESYKPAVAEGSGRAQTFRTILTVSSTATVALTVDNTMVMATVDYVDDKL
                     KEHEQSRRHPDASLTAKGFVQLSSATNSVSETQAATPKAVKAAYDLANGKYTAQDATT
                     ARKGLVQLSSATNSTSETQAATPKAVKAAYDLANAKYTAQDATTARKGIVQLSSATNS
                     TSETLAATPKAVKAAYDLANGKYTAQDATTARKGIVQLSSATNSTSETLAATPKAVKA
                     VMDETNKKAPLNSPALTGTPTTPTAPKGTNNTQIASTAYVMAAIAALVDSSPDALNTL
                     NELAAALGNDPNFATTMTKALAGKQPKDATLTALAGLATAADKFPYFTGNDVASLATL
                     TKVGRDILAKSTVAAVIE"
BASE COUNT          510 a          532 c          546 g          374 t
ORIGIN      
        1 ttcaccatca gcgaaggtgg gcgcaatgag taattcactg ttaccaccat cagccagcag
       61 tttcatgcgt tgtgccgaag ctgtcggaac gcgcattaca gacatcccgg tagacctcaa
      121 cacgctgtgg tcgccggaca cctgcccggt gcacctgctg ccttatctcg cctgggcatt
      181 ttccgttgac cgttgggatc gcaactggcc ggaagaggca aaacgacagg tgattcgtga
      241 tgcatggctg atacaccgac acaaagggac catcagcgca ctgcgcaggg ccattgagcc
      301 gctgggatac ctcattcgcg tgtctgagtg gtgggagttc ggcggagaac cgggaacatt
      361 taccgttgaa gtcggcacac tggacagtgg cgtgacggag gaaatgtatc tggaaatgga
      421 gcggttgatt gctgatgccc gtccggtcag ccgccacatg acagggctga atatcattca
      481 ggaaattccg ggggatattt ttgcagcggc ggcaacttat gacggtgaag ttattaccat
      541 ttatccggac gattaagcat gagtaccaca acacgaaaat ttaaaaccgt tatcaccgat
      601 acaggtgcca aaaaattagc tcaggcagcc gcgccagatg gtaagcctgt tcgcctgact
      661 catatggccg tgggcgacgg tggcggcacg ttgcccacac cagacagtaa gcagacccgt
      721 ctggtgcatg aggtgtggcg acacacagtt aatcgcgtca tcctggacgc aacacatcag
      781 aaccgcatta ttgcggagct ggttattcct cctgaaacgg gcggattctg gatccgggaa
      841 attggtgtat ttgatgagca cggcgattta atcgcggtgg gcaatactgc cgaaagttac
      901 aagccagccg ttgctgaagg gtccggtcgt gcacaaacat ttcgcaccat tctgaccgta
      961 tccagcacgg ccaccgtggc gcttaccgtg gataacacca tggtgatggc cacagtggat
     1021 tacgtggatg acaaactgaa agagcatgaa cagtcacgac gtcacccgga cgcctcgctg
     1081 accgcaaaag gctttgttca actcagtagc gccactaaca gcgtgtctga aacgcaggct
     1141 gcaacgccga aagcggttaa ggccgcatat gacctggcta acggaaaata taccgctcag
     1201 gacgccacga cggcacgaaa aggccttgtc cagctcagta gcgccaccaa cagcacgtct
     1261 gaaacgcagg ctgcaacgcc gaaagcagta aaggccgcat atgacctggc taacgctaaa
     1321 tacaccgcac aggacgctac cactgcgcgg aaagggatag ttcagctcag tagcgccacc
     1381 aacagcacgt ctgaaacgct tgcggcaacg ccaaaggctg ttaaggcggc atatgacctg
     1441 gctaatggca aatacaccgc acaggacgct accactgcgc ggaaagggat agttcagctc
     1501 agtagcgcca ccaacagcac atctgaaacg ctggctgcga cgccaaaagc tgttaaggcg
     1561 gtaatggatg aaacgaacaa gaaagcgccc ttaaacagtc ctgcgctgac cggaacgcca
     1621 accacgccaa ctgcgccaaa agggactaat aatactcaga tcgcaagcac ggcttatgtg
     1681 atggccgcga ttgccgcact tgtagactcg tcgcctgacg cactgaatac gttgaacgag
     1741 ttagcggcgg cgctgggaaa cgacccgaat tttgcgacca ccatgactaa agcgcttgcg
     1801 ggtaagcaac cgaaagatgc cactctgacg gcgctggccg ggcttgctac tgcggcagac
     1861 aagtttccgt attttacggg gaatgatgtc gccagcctgg caaccctgac aaaagtcggg
     1921 cgggatattc ttgcgaaatc gaccgttgcc gctgttatcg aa
//
LOCUS       DAZPEB010000333         1953 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZPEB010000333 DAZPEB010000000
VERSION     DAZPEB010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.339"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(25..>1953)
                     /gene="pdeF"
                     /locus_tag="ACHURJ_004203"
     CDS             complement(25..>1953)
                     /gene="pdeF"
                     /locus_tag="ACHURJ_004203"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFR1282895.1"
                     /translation="IIIIIIIIILCWGGYRVFTPRRNNVSHGDTRLISQRIFWQIVFP
                     ATLFLILFQFAAFVGLLASRENLVGVMPFNLGTLINYQALLVGNLIGVPLCYFIIRVV
                     RNPFYLRSYYSQLKQQVDAKVTKKEFALWLLALGALLLLLCMPLNEKSTIFSTNYTLS
                     LLLPLMMWGAMRYGYKLISLLWAVVLMISIHSYQNYIPIYPGYTTQLTITSSSYLVFS
                     FIVNYMAVLATRQRAVVRRIQRLAYVDPVVHLPNVRALNRALRDAPWSALCYLRIPGM
                     EMLVKNYGIMLRIQYKQKLSHWLSPLLEPGEDVYQLSGNDLALRLNTESHQERITALD
                     SHLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQR
                     RGAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDEL
                     ISPDSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFP
                     FEVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYAS
                     YARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIRE
                     AVLSLGIDYMQGYLIGKP"
BASE COUNT          572 a          471 c          440 g          470 t
ORIGIN      
        1 ttcattcaac gtatcaatta acggctacgg cttaccaata agataaccct gcatataatc
       61 gatccccaaa gagagcaccg cctcgcggat ctcttcgttt tcaacgtact ctgccactac
      121 ccgcattttc ttcattcgcg ccaggtggca aatcgatgcc actatctgat aatccagact
      181 atttgacaca atattgcgga taaaactgcc gtcaatttta agcagatcgg cattcacatt
      241 tttaagccgt gcatagctgg cgtagccggt gccgaaatca tcaatcgcaa tctggcagcc
      301 taattcctga agatgctgca aggtgatttg cgcctgctta acattggtca gagcattact
      361 ttcggtgact tcaaaaataa gttgccacgc ttcaatctga tatttagcca gcaactgact
      421 gacttcaaaa ggaaaacgag cctgacatac cgaggttgga gacagattaa tagcaaaacg
      481 gtgagcgggc atcttcgctc tgttttcagc cataaattgc agcgtatgct caatgaccca
      541 catgtcgata ctcgacgata aaccaaattc gtgcgcgacc ggcaagaagc tatcggggct
      601 gatcagttca tcattctcac ctttcatgcg cagaagaatt tcatggtaaa catcaccacg
      661 cataccggta atcggctggg ccatcaggaa aaaatggttg tgttccagcg cctgctgtag
      721 tcgattcatc atcgcgactt tatctttcaa ttcgcgttgc aaatacattg ccccgcgacg
      781 ctgcatattt tccggggcgt tggtcacgat ggaaagttcg gcgaccgtat ttagctctcc
      841 cagcagcagg tagatatgat tcactggcga gcgcacatag cagtaactga cgccaatctg
      901 cggttgcatc ggcatgccat cccaaaagaa acgaaattgc ttgagatggc tatccagtgc
      961 ggtaatgcgc tcctggtgcg attctgtatt cagtcgcagc gcgagatcgt tacccgaaag
     1021 ctgataaaca tcttcacccg gttccagcaa gggtgacagc cagtgagaaa gtttttgctt
     1081 gtattgaatc cgcagcatga tgccatagtt cttaaccagc atttccatgc cagggatgcg
     1141 taaataacaa agcgcagacc agggggcatc acgcaacgcg cgattcaggg cgcgaacatt
     1201 tggcagatga accaccgggt ccacatacgc aagccgctga atgcgtctga ctaccgctct
     1261 ctgacgggtt gccagtacag ccatataatt gacaataaaa gagaatacca gataactgga
     1321 ggaggttatg gtcagctgcg tggtatagcc aggataaatg ggaatgtaat tttgatagct
     1381 gtggatgctg atcatcaaca cgaccgccca gagcagagaa atcagcttat aaccatagcg
     1441 catcgctccc cacatcatca ggggcagcaa taatgacaag gtataattgg tgctaaaaat
     1501 tgtgcttttt tcatttaacg gcatgcataa cagcaacaat aaagcaccta atgccagtag
     1561 ccagagcgcg aactcttttt tggtgacttt ggcatcaacc tgctgtttta attgcgaata
     1621 atagctacgt aaataaaatg gatttcgcac tacccgaatg atgaagtagc acagcgggac
     1681 accgatcaga ttacccacca gtaaggcctg ataattgatt aaagtcccga ggttaaaagg
     1741 catgacaccg accagatttt ctctgctcgc caataatcct acaaatgcag caaactggaa
     1801 aagtatcaga aacagcgttg caggaaacac aatctgccag aatatacgct gggaaattaa
     1861 acgggtatca ccatgtgaaa cgttgttacg ccggggagta aagaccctgt aaccgcccca
     1921 acagagaatg ataatgataa tgataatgat aat
//
LOCUS       DAZPEB010000334         1912 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZPEB010000334 DAZPEB010000000
VERSION     DAZPEB010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.340"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            1254..1856
                     /gene="fimB"
                     /locus_tag="ACHURJ_004204"
     CDS             1254..1856
                     /gene="fimB"
                     /locus_tag="ACHURJ_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HFR1282896.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTPPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT          569 a          344 c          401 g          598 t
ORIGIN      
        1 agtataaaat ctcttctgat gtttaattga tttgaatttt cgtaagctat atcacttact
       61 caatccattt tacccagagt cataaaatag agtgtctgtt gatctcattt tcatattcct
      121 ttcagactga agtgcgtttt catcacccga tgagaaagaa ctgaagtgat gtttgccata
      181 gattgcgaga tgccctgtct cattgactat cttggtgcac ctttatacct gttataccag
      241 atcaaaaatc acgcaatcca tacaacaaaa ccagatttgc aattcgtgtc acaaaatatg
      301 tcgatctttt tctaagagga agatgccatg tgaagccaga cgaacacttg cggtggtctt
      361 caaaaactaa agatctttgt ttaaatgttt gttttataaa taatttattt gaagtctaaa
      421 caagggaagc tttgcaagct aactcagtga gcttggtgaa aatcagtgtt tacccgccat
      481 caggctgagc ataattctca tcatgaaata tgtttcctgg tttgtggctt gtaactggtc
      541 acttctgaag tcgatctgga gaggcttgtt gatgttggtg ttttcaggat gatgtttcac
      601 ttagtttgtt tgccgtatcg cccggcgaat ggctgtgatt gaggaaggtt taagtcgtag
      661 tgaccaaagc tatatttacc aacgaatgta gatgaaaaaa tcatctcctg cgttccccca
      721 tatctccagg ataaaaagga atgtaacaat ctcatggcgt aagctgacga atcagccagg
      781 aataatcgct agggacctaa gaattagcat gataatagcc actaagaaat tactgcgttc
      841 catgaaatag ccattttgtg gcaaatggag ttgactaata atgtcatatg tgagacggct
      901 agttgaacga atattaaatt ttgctgaatt ttttatgctg attttacttg ttacagaaca
      961 tatcacatga tatttagata agattagttg cattaatgat gaggggtatt attagattcg
     1021 tatccgattg ataaatatat aaaggtacat agcatgcaag agcatagcgt ttgtatggca
     1081 acgttattat aattaactgt tgctacccca tttaagttca ctcagaagaa ctggtccact
     1141 tacgttagtt attaagcaaa cgttcgcttt tataaacata atcaggataa aaatgttgga
     1201 ttattgctaa cccagcacag ctagtgcgcg tctgtaatta taagggaaaa acgatgaaga
     1261 ataaggctga taacaaaaaa aggaacttcc tgacccatag tgaaatcgaa tcactcctta
     1321 aagcagcaaa taccgggcct catgcagcac gtaattattg tctgactttg ctttgtttta
     1381 ttcatggttt ccgggcgagt gaaatttgtc gattgaggat ttcggatatt gatcttaagg
     1441 caaagtgtat atatatccat cgattaaaaa aaggcttttc aacaacgccc ccgctattga
     1501 ataaagaagt tcaggcttta aaaaactggt tgagtatccg tacttcgtac ccgcatgctg
     1561 agagcgagtg ggtattttta tcgcgtaagg ggaatccgct ttctcggcaa cagttttacc
     1621 atattatctc gacttccggt ggtaatgccg ggttgtcact ggagattcat ccgcacatgt
     1681 tacgccattc gtgtggtttt gctttggcga atatgggaat agatacgcga cttatccagg
     1741 attatcttgg gcatcgcaat attcgtcata ctgtctggta taccgccagc aatgcagggc
     1801 gtttttacgg catctgggat agagccagag gacgacagcg tcacgctgtt ttatagagat
     1861 agttgagata ccagggatgg tgttaaaaac tacattattc ttacgatata tc
//
LOCUS       DAZPEB010000335         1871 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZPEB010000335 DAZPEB010000000
VERSION     DAZPEB010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.341"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..642)
                     /gene="spa29"
                     /locus_tag="ACHURJ_004205"
                     /gene_synonym="spaR"
     CDS             complement(<1..642)
                     /gene="spa29"
                     /locus_tag="ACHURJ_004205"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HFR1282897.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFS"
     gene            complement(642..902)
                     /gene="spaQ"
                     /locus_tag="ACHURJ_004206"
     CDS             complement(642..902)
                     /gene="spaQ"
                     /locus_tag="ACHURJ_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HFR1282898.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLTIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            complement(917..1567)
                     /gene="spa24"
                     /locus_tag="ACHURJ_004207"
                     /gene_synonym="spaP"
     CDS             complement(917..1567)
                     /gene="spa24"
                     /locus_tag="ACHURJ_004207"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HFR1282899.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLIEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FIVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINVPA"
     gene            complement(1572..>1871)
                     /gene="spa33"
                     /locus_tag="ACHURJ_004208"
                     /gene_synonym="spaO"
     CDS             complement(1572..>1871)
                     /gene="spa33"
                     /locus_tag="ACHURJ_004208"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HFR1282900.1"
                     /translation="NNEAKINLSESNGESDHTEVSLALFNYDDINVKVDFILLEKKMT
                     INELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELVSIEDGYGIEISSWMVKE"
BASE COUNT          735 a          359 c          275 g          502 t
ORIGIN      
        1 ggaaaaagca ttcatctgcg gagcaaatct cgataataca ccaagtaata cttctgatag
       61 taacaatact atcataacag gactggctaa aataacagcc tgacttgcca ataaagtcag
      121 aaatgttaag atatttgaga cacggaaaga acattgagaa aataacgggc atatattata
      181 agacaattgt atggattcta aaataaagac cattccacca ctgtatagaa atacaactgc
      241 agaaaaaaga ttgaaaaatt ttgccaattc agacgtatca acaccattgg caggatcaat
      301 tgaactacta agcgttgccc cacgctggtt gtcaataata ctaccaacag catgaaatat
      361 ccaaaaggga agagacaagc aaaaagagag aaggagacca acaattattt ccttgaacat
      421 aaggaaataa acatgttcaa aaacagaaga acctatgtct accttaccag aagtaattaa
      481 tcctgatgca acaagaaaaa tcacaggtat tctaatagat ggagaaatta taccgttatt
      541 taaaaatgga agaaagaaaa ataatggagc caatctaaaa aaaacgccgt tcaggagtat
      601 taaaaacaca tgaatacttt cgaaccagct tgaaatgtcc atcaaacccc actcttaatt
      661 aaaaacatta tttcatgaca aaaagacaat aaaacctcac cataccatcc agaaagaagt
      721 agcaaagata ttgagacacc tataagcttt ataccaaaag gaagtgtctg ctcttgaagt
      781 tgagtcactg tctgtaataa accaatagtt aatccaataa ccgtagctat acctactggc
      841 cataaggaaa agataaggat taaataaaga gccttattac ccatataaac tatatcagac
      901 acattacctc catcatctaa gcaggaacat tgatatattg ttcaatcaag gccttagaca
      961 ggatccccca cccatcaagc gcaacaaata atacaagttt tattggtact gatattgtga
     1021 ttggactcat catcatcata ccaagtgcaa gtaaaatgct tgaaataaca agatcaacaa
     1081 caataaatgg caaatataaa taaaaaccta ttttgaacgc atctttaatt tcacttagcg
     1141 catatgcagg caagagggaa aacaatgaat aatcattatt ctctgcactc tttaaatcag
     1201 cattttcttc ttcagacctt tggaaaaaac gtgccaactc aagatctgta tgctttttca
     1261 agtattgctt gtattcaatc aagccagaat cagaaaaacg gacaatatct gatattgtat
     1321 caaatttttg cgggccattt agataatttt cataaccagc ttcaataatg ggtttcatca
     1381 cgaacaaagc cataatcaat gcaataccat tgagagtcat atttgatgga acctgctgca
     1441 aaccaagtgc atttcgtacc attacaaaaa caatagaaaa ctttatataa caagtaccag
     1501 cagcaactaa aaatggtaat agcgtaaaaa aagaaagtgt agcgatgagg gacatatcac
     1561 tcagcatgag attactcctt taccatccaa gaactaatct cgataccata accatcctca
     1621 atagaaacaa gttccccatg cccaaccaaa gaaccattta ctttaatatt tacatgttta
     1681 actatgtcat cgggaaactt gaataattcg ttttctacat acatttttag ttcattgatt
     1741 gtcatctttt tttctaaaag gataaagtcc acttttacat tgatatcatc ataattgaat
     1801 aatgccaaag aaacttctgt gtgatctgac tcaccattac tttctgacag attaattttt
     1861 gcctcattat t
//
LOCUS       DAZPEB010000336         1830 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZPEB010000336 DAZPEB010000000
VERSION     DAZPEB010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.342"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            26..556
                     /locus_tag="ACHURJ_004209"
     CDS             26..556
                     /locus_tag="ACHURJ_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFR1282901.1"
                     /translation="MSNSLLPPSASNFMRCAEAVGTRITDIPVDLNTLWSPDTCPVHL
                     LPYLAWAFSVDRWDRNWPEETKRQVIRDAWLIHRHKGTISALRRAIEPLGYLIRVSEW
                     WEFGGEPGTFTVEVGTLDSGVTEEMYLEMERLIADARPVSRHMTGLNIIQEIPGDIFA
                     AAATYDGEVITIYPGD"
     gene            559..>1830
                     /locus_tag="ACHURJ_004210"
     CDS             559..>1830
                     /locus_tag="ACHURJ_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFR1282902.1"
                     /translation="MSTTTRKFKTIITDTGAKKLAQVAAPDGKPVRLTHMAVGDGGGT
                     LPTPDSKQTRLVHEVWRHTVNRVILDATHQNRIIAELVIPPETGGFWIREIGVFDEHG
                     DLIAVGNTAESYKPAVAEGSGRAQTFRTILTVSSTATVALTVDNTMVMATVDYVDDKL
                     KEHEQSRRHPDASLTAKGFVQLSSATNSVSETQAATPKAVKAAYDLANGKYTAQDAST
                     TRKGLVQLSSATNSTSETQAATPKAVKAAYDLANAKYTAQDATTAQKGIVQLSSATNS
                     TSETLAATSKAVKAVMDETNKKAPLNSPALTGTPTTPAARQGTNNTQIASTAFVMAAI
                     AALVDSSPDALNTLNELAAALGNAPNFATTMTNALAGKQPKDATLTALAGLATAADRF
                     PYFTGNDVASLATLTKVGRDILAKSTVAAVIE"
BASE COUNT          470 a          495 c          509 g          356 t
ORIGIN      
        1 ttcaccatca gcgaaggtgg acgcaatgag taattcgttg ttaccaccat cagccagcaa
       61 tttcatgcgt tgtgccgaag ccgtcggaac acgcattaca gacattccgg tagacctcaa
      121 cacgctgtgg tcgccggaca cctgcccggt gcatctgctg ccttatctcg cctgggcgtt
      181 ttccgttgac cgctgggatc gcaactggcc ggaagagaca aagcgacagg ttattcgtga
      241 tgcatggctg atacaccgac acaaagggac catcagcgca ctgcgccggg ccattgagcc
      301 gctgggatac ctcattcgcg tgtctgagtg gtgggagttc ggcggagaac cgggaacatt
      361 taccgttgaa gtcggcacgc tggacagtgg cgtgacggag gaaatgtatc tggaaatgga
      421 gcggttgatt gctgatgccc gcccggtcag ccgccacatg acagggctga atatcattca
      481 ggaaattccg ggggatattt tcgcagcggc ggcaacttat gacggtgaag ttattaccat
      541 ttatccaggc gattaagcat gagtaccaca acacgaaaat ttaaaaccat tatcaccgat
      601 acgggtgcaa aaaaattagc tcaggtagcc gcgccagatg gtaagcctgt ccgcctgact
      661 catatggccg tgggcgacgg tggcggcacg ttgcccacac cagacagtaa gcagacccgt
      721 ctggtgcatg aggtgtggcg acatactgtt aatcgcgtca tcctggacgc aacacatcag
      781 aaccgcatta ttgcggagct ggttattcct cctgaaacgg gcggattctg gatccgggaa
      841 attggtgtat ttgatgagca cggcgattta atcgcggtgg gaaatactgc cgaaagttac
      901 aagccagccg ttgccgaagg gtccggtcgt gcacaaacat ttcgcaccat tctgaccgta
      961 tccagcactg ccaccgtggc gcttaccgtg gacaacacca tggtgatggc cacagtggat
     1021 tacgtggatg acaaactgaa agagcatgaa cagtcacgac gtcacccgga cgcctcgctg
     1081 accgcaaaag gctttgttca actcagtagc gccactaaca gcgtgtctga aacgcaggct
     1141 gcaacgccga aagcagtaaa ggccgcgtat gatcttgcta acggtaaata tactgcgcag
     1201 gatgccagca cgacgcgaaa aggccttgtc cagctcagta gcgccaccaa cagcacgtct
     1261 gaaacgcagg ctgcaacgcc gaaagcagta aaggccgcgt atgaccttgc taacgcaaaa
     1321 tataccgctc aggacgccac gacggcacaa aaagggatag tccagctcag tagtgccacc
     1381 aacagcacgt ctgaaacact ggccgcgaca tcgaaagcgg ttaaggcggt aatggatgaa
     1441 acgaacaaga aagcgccctt aaacagtcct gcgctgaccg gaacgccaac aacgccagct
     1501 gcgcgacagg gaacgaataa tacccaaatc gcaagcacgg ctttcgttat ggctgcgatt
     1561 gccgcccttg tagattcgtc acctgacgca ctgaatacgc tgaacgagtt agcggcggcg
     1621 ctgggaaacg ccccgaattt tgcgaccacc atgactaacg cgcttgcggg taagcaaccg
     1681 aaagatgcca ccctgacggc gctggccggg cttgctactg cggcagacag gtttccgtat
     1741 tttacgggga atgatgtcgc cagcctggca accctgacaa aagtcgggcg ggatattctt
     1801 gcgaaatcga ccgttgctgc cgttatcgaa
//
LOCUS       DAZPEB010000337         1817 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZPEB010000337 DAZPEB010000000
VERSION     DAZPEB010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.343"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..299)
                     /locus_tag="ACHURJ_004211"
     CDS             complement(<1..299)
                     /locus_tag="ACHURJ_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001345656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR1282903.1"
                     /translation="MNLLFHTLLFCFFYLTCFIPNFIRLKIKCAAGYNFAFRYFRTSY
                     LPRIIPRFTDAPIILAKDLSMAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYAN"
     gene            complement(317..1465)
                     /locus_tag="ACHURJ_004212"
     CDS             complement(317..1465)
                     /locus_tag="ACHURJ_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1282904.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQVRLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWSIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT          575 a          464 c          394 g          384 t
ORIGIN      
        1 tggttggcgt agttattaac aacgttgcca aaaccccaga cagtgataaa agacatcagc
       61 gcaatgttgt accatcgcag ctgtttttcc tgaacattcc cagccataga caaatccttc
      121 gccaaaatta taggggcatc tgtaaaacga gggattatgc gcggcaaata agaagtgcgg
      181 aaatagcgaa atgcaaaatt ataacctgct gcacatttta tttttaaacg aataaaattt
      241 ggaataaagc aagtcagata gaagaaacaa aaaagcagag tgtgaaacag taagttcaca
      301 ctctgcttga attcatttac acgtgagcaa cgggtttcgg cgtatgaccg gcgttgcgca
      361 gcagcatcag caaatagata aacgatacgc tggcgatcat gataaacaat aaattatcgg
      421 agaaattctg cattagcata gcggtaaatg acgggccaag cagacttccc acagtatagc
      481 tcaacagtaa ggcctggttc atcgccacca gttgatgatg ttcaactttc tcgcaagccc
      541 atgccatcgc caccggatat agcgtaaagc cagcggcacc gaggatgaat aacgctgggg
      601 ccatcgccgc ctggctaagc atcgcgatac tgccgagaat gacgacaaag acctgaacac
      661 gcaacaccag cagtcgacca aacttatccg ccagacgtcc aatcgaccat tgtccaagga
      721 tacccgcact gaccagtacc gccatccaga aaccaatgct ggcattgctc acccctttgt
      781 gattgaggta cagcggcatc aggccatata gagatcccag aacgattcct gagataatgc
      841 agccattcac gccaagccgc acctgacgga gttttagcat tgacgtaatt gacgtcgaat
      901 catggttttc agcctgctga ttcagcacac gcgtaaacaa cagcggtaag atccctgcca
      961 acgtcaaacc tgtaacccac ggcaatacgg acatcagctc ggttgaaact ttgctgacca
     1021 gtaactggcc taaaaacgtt cccacgtaat aaaccatcat atacgcagca agcaaacgcc
     1081 cacggttacg tgacgtccca ctgcacatca gcgcgctctc aacaaccacc caaatcatgg
     1141 cacagccgac gcccgcgaca aaacgccaag ccaaccagct ccagaatcca atcatcaggc
     1201 caaggccggc acagccagcg gcaaaaatga aggaggccag ataatagctg cggttaaagc
     1261 caatgcgctt aatgacatac cctgtcagca atgtaccgac aaggttgccg gtaaaatagg
     1321 atgagctgac aacgcctacc tgccatgtgg acatgtgttc ctgggcgagc caaagcggca
     1381 cgagtgtatt taacaccgca atcgccagag tcaacaaaag caggccagac agcaaaagca
     1441 tgacaggccg ggtatacgtg gacatgaatt aaaaccgtga ggaagttcaa atttcatgcg
     1501 catcatgcca ccgccaaaac attgtcaatg agtgaaaata cgcgatatcc cagcggcggt
     1561 attatcgatt tatattactc attcataacg ctaatgctga agagatgatt agtctcatct
     1621 gattgttttt attgaactaa cacaaatttc atacgcttcg ttcagtcgaa aaatttcatg
     1681 gtcacagcaa actcatattt cccgtctatg ctttcagagc aatgcatcgc gtctgatcga
     1741 tttcagacgt accactgact ctcgaaagga gaagaggatg accaaaccgt atgttcgtct
     1801 tgataaaaat gatactg
//
LOCUS       DAZPEB010000338         1764 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZPEB010000338 DAZPEB010000000
VERSION     DAZPEB010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.344"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..279
                     /gene="dmsB"
                     /locus_tag="ACHURJ_004213"
     CDS             <1..279
                     /gene="dmsB"
                     /locus_tag="ACHURJ_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFR1282905.1"
                     /translation="PQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDE
                     LRKKHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            281..1144
                     /gene="dmsC"
                     /locus_tag="ACHURJ_004214"
     CDS             281..1144
                     /gene="dmsC"
                     /locus_tag="ACHURJ_004214"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFR1282906.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLIVTMVLGVIFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVVSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(1179..>1764)
                     /gene="ycaC"
                     /locus_tag="ACHURJ_004215"
     CDS             complement(1179..>1764)
                     /gene="ycaC"
                     /locus_tag="ACHURJ_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFR1282907.1"
                     /translation="VLLVDHQAGLLSLVRDIEPDKFKNNVLALGDQAKYFNLPTILTT
                     SFETGPNGPLVPELKAQFPDAPYIAHPGNINAWDNEDFVKAVKATGKKQLIIAGVVTE
                     VCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGAQLMTWFGVACELHR
                     DWHNDIEGLATLFSNHIPDYRNLMTCYDTLTKQK"
BASE COUNT          379 a          425 c          490 g          470 t
ORIGIN      
        1 ccgcaatata acgaaacgaa aggccatatg accaaatgcg atggttgtta tgaccgtgtt
       61 gcggagggta aaaagccgat ctgtgttgaa tcttgtccgc tgcgggcgct ggatttcggc
      121 cctatcgacg agctgcgtaa aaaacatggc gatctggcgg ccgttgcgcc gttgccgcga
      181 gctcacttta ccaaaccgaa tattgtgatc aaacccaatg ccaatagccg cccgaccggg
      241 gataccactg gctatctggc aaacccgaag gaggtgtgag atgggaagtg gatggcatga
      301 atggccgctg atgatcttca cggtcttcgg gcaatgtgta gcaggtggtt ttatcgttct
      361 ggctttggcg ctgctcaaag gcgacctgcg agcagaagcc cagcagcgtg ttatcgcctg
      421 catgtttggt ttatgggtgc tgatgggcat tggctttatc gcctctatgc tccatcttgg
      481 ttcaccaatg cgcgctttta actcgctcaa ccgggtaggg gcttcagcac tcagtaacga
      541 aatcgccagc ggttcgatct tttttgccgt aggcggcatc ggctggttgc tggcaatgct
      601 gaaaaagctg tcaccggcat tgcgtacgct gtggctgata gtgacgatgg ttcttggcgt
      661 catctttgtc tggatgatgg tgcgtgtgta taacagcatt gataccgttc cgacctggta
      721 cagcatctgg acgccgatgg gcttcttcct gacgatgttt atgggcggcc cgttactggg
      781 ttacctgttg ttgagtctgg ctggcgtcga tggctgggcg atgcgtctgc tgccagcaat
      841 ttctgtactg gcactggtag tgagtggcgt ggtgtcagtg atgcagggcg cagagctggc
      901 gactattcat agttctgtgc agcaggccgc agcgctggtg ccggactatg gtgcgctgat
      961 gtcctggcgg atcgtgcttt tggccgttgc cctgtgcttg tggattgcac cacagctaaa
     1021 aggttatcag cctgcggttc cgctactttc tgtatcattc attctgcttc tggcagggga
     1081 attaatcggt cgcggcgtat tctatggttt gcatatgacc gtggggatgg ccgtcgcaag
     1141 ctaatcataa caaccggggt ttcggccccg gttctctttt atttctgctt cgttaacgtg
     1201 tcataacagg tcatcaaatt acgataatca ggaatgtggt tagagaacaa cgtcgccagt
     1261 ccttcaatat cattatgcca gtcgcgatgc agctcgcacg ccacgccgaa ccatgtcatc
     1321 aattgcgcac cagcttgcga catgcgatcc catgccgaat gccgggtaat ttcattaaag
     1381 gtccctgacg cgtcagtcac gacaaagacg tcaaaccctt cttcaatggc tgaaagcgcc
     1441 gggaatgcca cgcaaacttc ggttaccaca ccggcaataa ttaactgttt tttacctgtc
     1501 gctttgacag cttttacaaa atcttcgtta tcccaggcgt taatatttcc cgggtgagca
     1561 atgtaaggtg catcggggaa ttgtgctttc agttctggaa ctaatgggcc gttaggaccg
     1621 gtttcaaaac tggtggtaag aatagttggt aagttgaaat acttagcctg gtcacccagc
     1681 gccagcacgt tatttttaaa cttatcgggt tcgatatccc gtacaaggga aagtaaacca
     1741 gcctggtgat caacaagcaa aacg
//
LOCUS       DAZPEB010000339         1757 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZPEB010000339 DAZPEB010000000
VERSION     DAZPEB010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.345"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..464
                     /gene="virK"
                     /locus_tag="ACHURJ_004216"
     CDS             <1..464
                     /gene="virK"
                     /locus_tag="ACHURJ_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HFR1282908.1"
                     /translation="LTQNGHIAFIGGLQGAPKNTGPDVIRCATRACYGIFPKRIIFEA
                     FCALMKACNISECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYRL
                     PTQVIRKPLSDIASKKRSEYRKRYALLDYIHETTIRSLDAYPVHSEHQNLN"
     gene            529..1473
                     /gene="lpxM"
                     /locus_tag="ACHURJ_004217"
                     /gene_synonym="msbB"
     CDS             529..1473
                     /gene="lpxM"
                     /locus_tag="ACHURJ_004217"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFR1282909.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWSTWFLLGMIAGISMFPPSFRDP
                     VLAKIGRWAGRLSKKARRRATINLSLCFPEKNDTEREIIVDKMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGKKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHAREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHLLTIEIRPPMDACIASADNKMI
                     ARQMNKTVEILVGPHPEQYVWVLKLLKTRKSNEADPYP"
     gene            1654..>1757
                     /locus_tag="ACHURJ_004218"
     CDS             1654..>1757
                     /locus_tag="ACHURJ_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR1282910.1"
                     /translation="MSRKTQRYSKEFKAEAVRTVLENQLSISEGASRLS"
BASE COUNT          535 a          345 c          398 g          479 t
ORIGIN      
        1 ttctgactca aaatggacac attgctttta ttggcggatt acagggagcc cctaaaaata
       61 ccggacctga tgttattcgg tgcgcaaccc gggcctgtta tgggattttc ccaaaacgga
      121 ttatttttga agcattttgt gcgttgatga aagcttgcaa tatctctgag tgtctggcag
      181 tcagtgagca cagtcatgtt ttccggcaat tgagatactg gtatcagaaa cgcaaaacct
      241 ttgttgcggt ctatagtgat ttctgggagt ctgtagcagg aaaaacctgt ggtgactggt
      301 acagattacc aacccaggtg attcgaaagc cgctaagcga tatagccagt aaaaaacgct
      361 ctgagtaccg aaaacgatac gctttgctgg attatatcca tgaaactact atccgctctt
      421 tggatgcata tcctgtacac tcagaacatc agaatttaaa ttaaggtttg atgtatcctc
      481 tgaataaaac agtaatgata attttggtag aggcattcgc actaaataat gaaaaaatat
      541 aaatctgagt ttattcctga atttaagaaa aattaccttt cccctgttta ctggtctaca
      601 tggttccttt tgggaatgat tgcaggtatt tcaatgtttc ccccttcatt cagagatcct
      661 gtcttggcaa aaatagggcg ttgggcgggc agattgagca aaaaagctcg tcgcagggcg
      721 acgattaatt tatcgctttg tttcccagaa aagaatgata cagaacggga aataattgtc
      781 gacaaaatgt ttgccacagc attgcaatcc atagtgatga tggcagaact ggcaattcgt
      841 ggtccggaaa agttccagaa acgagtgttc tggaaaggcc tcgaaattct tgaggaaatt
      901 cgacacaata atagaaatgt gatttttctg gttccccatg gctggagcgt ggatattcct
      961 gcaatgttgc tggcagctca gggaaaaaaa atggccgcca tgtttcatca gcaacgaaat
     1021 ccagtgattg attatgtctg gaattcagta cggcgtaaat tcggggggcg cttacatgcc
     1081 cgggaggatg ggataaaacc atttattcag tcagtacgcc agggatactg gggatattac
     1141 ctcccagatc aggatcatgg tcctgaatac agtgaatttg ctgatttttt tgcgacctat
     1201 aaagcgacat taccaatcat tggacgtctg atgaacatca gtcaggctat gattatacca
     1261 cttttcccgg tttatgatga aaaaaaacat ctcctgacta ttgaaattcg gccaccaatg
     1321 gatgcttgca ttgccagcgc ggacaataaa atgattgccc gacaaatgaa caaaacagtg
     1381 gaaattttgg tggggccaca tccggaacag tatgtctggg ttttaaaatt gttaaaaaca
     1441 cgcaaatcaa acgaagcgga cccgtaccct tgaaattata aagtataagt atttccacta
     1501 gttgtttgta agcgtacagc ctgaaccgtc tggtcagaat ctgacgaatt agacaaagtg
     1561 gtgtccacca aataagtagt gggaaccaat gaggtagcct gagtttaacg gacactcctt
     1621 cctgaaatag aatggcatca gaaggagcta ataatgagca gaaaaaccca acgttactct
     1681 aaagagttca aagccgaagc tgtcagaacg gttcttgaaa atcaactttc gatcagtgaa
     1741 ggcgcttccc gattatc
//
LOCUS       DAZPEB010000340         1736 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZPEB010000340 DAZPEB010000000
VERSION     DAZPEB010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.346"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            67..807
                     /locus_tag="ACHURJ_004219"
     CDS             67..807
                     /locus_tag="ACHURJ_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFR1282911.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGTIEGNASL"
     gene            842..1736
                     /locus_tag="ACHURJ_004220"
                     /pseudo
     CDS             842..1736
                     /locus_tag="ACHURJ_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
BASE COUNT          502 a          367 c          369 g          498 t
ORIGIN      
        1 aatagtgtta ataatttcta tttttatatt attccctgtt ttaattaact ctgtcaggga
       61 tggtttatgt tttttaacac caaacataca acagctttat gcttcgtaac ctgtatggct
      121 tttagttcat cgtctattgc ggacattgtc atttcgggta ctcgcgtaat atataaaagc
      181 gatcaaaaaa gtgtcaacgt acgtctggaa aataaaggga ataacccgtt gcttgtccag
      241 agttggttag atactggcga tgacaacgct gagcctggca gtattacagt cccttttact
      301 gccacgccgc cagtatcgcg tattgatgcc aaacgtgggc aaacaatcaa attaatgtac
      361 acagccagca cctcactgcc taaagacaga gagagcgtgt tctggtttaa cgtactggaa
      421 gttccaccaa aaccagatgc agaaaaggtc gcgaatcaaa gtctgctgca actggcattt
      481 cgcacacgta taaaactttt ctatcgcccg gatggattga agggaaatcc ctctgaagcc
      541 ccgttagccc tgaagtggtt ctggtcaggt tcagaaggta aggcgtcatt gcgagtgacc
      601 aatccaaccc cttactacgt ctcttttagc agtggtgatt tagaagctag cggtaaacgc
      661 tatccgattg atgtgaaaat gattgcacca tttagtgatg aggtcatgaa agtcactggc
      721 cttaatggca aagcaagttc tgcaaaagta catttttacg ccattaatga ttttggtggc
      781 accattgaag gtaatgccag tctgtaatca ggcaaggata taattccgca ggaagcataa
      841 cgtgactata aaaccaacta aaaattatca taatcatctg acccgtatcg ctacgttttg
      901 cgcgctgctg tattgcaata gtgctttctg tgctgaactt gttgaatatg accatacctt
      961 cctgatgggg cagaatgcct caaatattga tctcagccgg tatagtgaag gtaaccctgc
     1021 tataccgggt atgtacgacg tcagtgttta tgtcaacgat caacctatta ttaaccaaag
     1081 cattacattt attgaaattg aaggaaaaaa aacgcacaag cctgtatcac acttaagaat
     1141 ttattgcagt ttcatataaa ttctcccgat ataaataacg aaaaagccgt tctgcttgcc
     1201 agggatgaaa cactcggcaa ttgccttaat ttgacggaaa ttattcctca ggcttctgtt
     1261 cgttatgacg ttaacgagca acgtctggat atagacgttc ctcaggcatg ggtaatgaaa
     1321 aattaccaaa actatgttga tccatcgtta tgggaaaatg gcattaatgc ggccatgttg
     1381 tcatacaacc tcaacggata tcatagtgaa actcctggtc gaagaaatga cagcatttat
     1441 gctgctttta acggtgggat gaatttaggt gcatggcgac tgcgtgcctc aggcaactac
     1501 aactggatga ccgattctgg cagtaattat gattttaaga atcggtatat acagcgtgat
     1561 atcgcctcgc tgcgttctca actcattctt ggtgagtctt atacaacggg cgaaaccttt
     1621 gattctgtca gtatccgcgg cattcgttta tacagcgaca gccgcatgtt gcctccgaca
     1681 ttagcgagtt ttgcgcctat cattcatggc gttgctaata ccaacgccaa agtcac
//
LOCUS       DAZPEB010000341         1715 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZPEB010000341 DAZPEB010000000
VERSION     DAZPEB010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.347"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            122..1399
                     /locus_tag="ACHURJ_004221"
     CDS             122..1399
                     /locus_tag="ACHURJ_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1282912.1"
                     /translation="MNKIKNYRWHMIALVCFITVINYLDRTALGIAAPTIMETTGITK
                     EQYSWIVSAFQLAYTLGQPVMGFFIDTVGLKLSFAICAAIWGLATMGHALTGTWSGLA
                     FMRALMGFSEASAIPAGVKTASTWFPAKERGVATGVFNMGTSLGAMLAPPLIAWCIMF
                     HSWQFAFIVSGSLALLAALFWFFCYKDPKDAKRLSDEERHYIESGQEQHLKTDKKEKT
                     SIKHILSQRNFWGIGIARFLADPAWGTINFWVPIFFVETLHFSLKEIAMFVWLPFLLG
                     DLGCLASGFVAKFFHDRGVSLINSRRITFTIAAVIMMTIGLVSIVENPYIAVLLISIG
                     AFSHQCLSTVAATLGGDLFKKDEVATAVGMAGACAWSGQLIFNLFIGAFVHIIGFAPF
                     FIALAFFDIIGAIALWTLIKVKDEEPQVQLATH"
BASE COUNT          447 a          368 c          372 g          528 t
ORIGIN      
        1 ttttaaaggg agaaatttac acacaataat tttcaatatt tcctctgtac ctgattttta
       61 ttacctatta cacggaaata ccaacaacca gggtttatta atataattaa aaggtttaaa
      121 catgaataaa ataaagaatt accggtggca tatgattgcc ctcgtatgct ttatcactgt
      181 aatcaattat ctggacagaa cggcgttagg tattgcggct ccaacgatta tggagacaac
      241 cggaataact aaagagcaat attcatggat tgtcagtgca ttccagttgg cctatacatt
      301 agggcaaccg gtaatgggct tctttattga taccgtgggt ctgaagttaa gttttgcgat
      361 atgtgccgca atttggggcc tggcgacaat gggccatgca ctaaccggaa cgtggtctgg
      421 tctggcattt atgcgcgccc tgatgggttt cagcgaagcg tcggcaattc cggcgggtgt
      481 aaaaaccgca tcaacatggt tcccggcaaa agagcgtggc gtggcgacag gtgttttcaa
      541 tatgggcacc tcactcggcg cgatgcttgc tccaccgttg attgcctggt gcattatgtt
      601 tcatagctgg caatttgcgt ttattgtttc aggtagtctt gctttgctcg cggctttatt
      661 ttggttcttt tgttataaag atccgaaaga tgccaaacgc ctttctgatg aagagcgcca
      721 ctatattgaa tcagggcaag aacagcatct taaaacagat aagaaagaaa aaacgtcaat
      781 caagcatatc ctcagccaac gtaatttctg ggggattggc atcgcgcgtt ttctcgcaga
      841 cccggcatgg ggaaccatta acttctgggt gccgattttc ttcgtcgaaa cgctgcattt
      901 tagcctgaaa gaaattgcca tgttcgtctg gctgcctttc ctgctgggcg atctcggctg
      961 tttagccagt ggttttgtcg cgaagttctt ccacgatcgc ggcgtgagtt taattaactc
     1021 acgaagaatt accttcacta ttgcagccgt cattatgatg acgattggcc tggtgagtat
     1081 tgtcgaaaat ccctacattg ccgtattact gattagtatt ggcgcgttct cgcatcaatg
     1141 tctttctact gtagcagcaa ctctgggtgg cgatctgttc aaaaaagacg aagtagctac
     1201 cgcagtgggt atggcaggag cctgtgcgtg gagcggtcag ttgattttca acctgttcat
     1261 cggggcattc gttcacatta tcggcttcgc gccgttcttt attgccctgg ctttctttga
     1321 cattattggt gctattgcgt tgtggacgct tatcaaagtt aaagatgaag aaccacaagt
     1381 acagttagct acccactaat tattttaaat aaaaacgcta taattgaggt tatggcgttt
     1441 ttaaagaagc ttgagttatc gaaccccgct taccaacgcg ttgtcagtca tctcccccct
     1501 ccctggtgca tcgttatatc cgtgatgagc gatttttgat attaatatct ttcaccatcc
     1561 gctattttca ccacttcaga aaacaaactt atttttatta atatcaattc tttttctttg
     1621 aacattcaac tgcgatattt gtaaaaacat taaacaggca acaataaaat gacattacca
     1681 acaactattt attcattccc cgcatattta cctcg
//
LOCUS       DAZPEB010000342         1665 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZPEB010000342 DAZPEB010000000
VERSION     DAZPEB010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.348"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(8..868)
                     /locus_tag="ACHURJ_004222"
     CDS             complement(8..868)
                     /locus_tag="ACHURJ_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="HFR1282913.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            complement(1051..1608)
                     /locus_tag="ACHURJ_004223"
     CDS             complement(1051..1608)
                     /locus_tag="ACHURJ_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005687610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR1282914.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTD
                     REGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGD
                     MGQMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTG
                     ATEIAHQLSIARSTVYKILEDERAS"
BASE COUNT          422 a          436 c          364 g          443 t
ORIGIN      
        1 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
       61 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
      121 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
      181 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
      241 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
      301 tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg
      361 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
      421 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gcagcagtgt
      481 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
      541 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
      601 cgagttgctc ttgcccggcg tcaacacggg ataataccgc accacatagc agaactttaa
      661 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
      721 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
      781 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
      841 gggcgacacg aaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
      901 accagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
      961 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     1021 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt caagaatttt
     1081 ataaaccgtg gagcgggcaa tactgagctg atgagcaatt tccgttgcac cagtgccctt
     1141 ctgatgaagc gtcagcacga cgttcctgtc cacggtacgc ctgcggccaa atttgattcc
     1201 tttcagcttt gcttcctgtc ggccctcatt cgtgcgttct aggatcctcc ggcgttcagc
     1261 ctgtgccaca gccgacagga tggtgaccac catttgcccc atatcaccgt cggtactgat
     1321 cccgtcatca atgaaccgga ctgccacgcc ctgagcgtca aattccttta tcagttggat
     1381 catatcggca gtgtcgcggc caagacggtc gagcttctta accagaatga catcaccttc
     1441 ctccaccttc atcctcagca aatccagccc ttcccggtct gttgaactgc cggatgcctt
     1501 atcggtaaat atacggtttg ctttcacacc tgcgtctttg agtgctctga cctgaagatc
     1561 aagagactgc tgactggttg agacccgagc gtaaccaaaa agtcgcataa aaatgtacct
     1621 taaatcgaat atcggacaac tcatgtctat tattacaaat ttacg
//
LOCUS       DAZPEB010000343         1658 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZPEB010000343 DAZPEB010000000
VERSION     DAZPEB010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.349"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(56..343)
                     /locus_tag="ACHURJ_004224"
     CDS             complement(56..343)
                     /locus_tag="ACHURJ_004224"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282915.1"
                     /translation="MNRTAFRAIHAVGVDTGRNCRKFPLRAAGNFRIPFVINTYKPED
                     QFFVSGIHKERNIQVCATLNGIVKIIKAQNLSFFVTGSFKPSGLFPPAPQK"
     gene            421..850
                     /locus_tag="ACHURJ_004225"
                     /pseudo
     CDS             421..850
                     /locus_tag="ACHURJ_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1037..1274
                     /locus_tag="ACHURJ_004226"
                     /pseudo
     CDS             1037..1274
                     /locus_tag="ACHURJ_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            1388..1646
                     /locus_tag="ACHURJ_004227"
                     /pseudo
     CDS             1388..1646
                     /locus_tag="ACHURJ_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
BASE COUNT          471 a          323 c          476 g          388 t
ORIGIN      
        1 cagtgatcca ggtccggtat tccatacgat agtgtccact tcaaaatgat ggacattatt
       61 tttgtggagc cggaggaaac agaccagacg gtttaaatga gccggttacg aagaagctga
      121 ggttctgcgc tttgataatt ttgacgatgc cgttgagtgt tgcgcagacc tgaatattcc
      181 gttctttgtg gatgccggaa acaaaaaact ggtcttctgg tttgtacgtg ttgatgacga
      241 agggtatccg gaaattgccc gctgcacgga gcgggaattt gcgacaattc ctgccggtat
      301 caacgccgac ggcatgtatt gccctgaatg cggtacggtt cactggccgg atggcgttat
      361 tccgcctttc tgatgcttcc ccgttttgcc gatatttttc agcagggaaa ccgatggctt
      421 aactggctgg agaagcagcc ggtgcagatg agccgcctgg aacattatgc cggtcaggat
      481 gaaattggcc tgcgttacaa cagccaccgg acaaaacgag aagaaaacct tgtgatgagt
      541 ggcgacgagt ttatggaaag gttctcctgg catgtggcgg ataaggggtt ccggatggtg
      601 atacggggtc ctgagtccgg tgaagcggcg attactggaa gatgtggtgt acgccataac
      661 ggagacagtg agaaaaacgg cgaggcaaat cacaaggaga gggatgtatc agcagtgact
      721 gaaggttgac ccgctgaagt gcgagctgtg cggaggtcag atgcggttta cggggctgaa
      781 gaggggttac cgtctggcag agcaggttat gatgcatgag ccgctggctc gaatacggtg
      841 gtgcggctga gagtcgcaga ggggaagttg cgtccatgtt gaggggaacg gagcaaaaaa
      901 ccatcagaga tacattgaat caatcaatgt aagcaatatt aagtgtctat accgattact
      961 tgatgatcag gttgtgttga ataaacgcgc attcttcggt gcttttttaa tcttttgggg
     1021 atttaaattc ctatcgatga actgtcgata tgaaaaagcg agcatcatac tgacgtcaaa
     1081 caaaggggtc gcagactggg gagaaatgtt cggagatcac gtgctggcaa cagcgatcct
     1141 agatcggctg ctacacgctg aatatcaaag gagagagtta ccggttaaaa gagaaacgta
     1201 aagctggagt gctgaccaaa aacacaacgc caatcagtga tgatgaaatg gtggaaaacg
     1261 gacagcatca gtaacgaaag tatcttagca ggcatgaaaa tcgtccgcat cccctaaacg
     1321 gtcaaaatgc ttctgccgtg aaggagggag aatgccatct catcacttat atctggcgaa
     1381 tgaaacacag atggtcggcc ttgcccccaa acacacggcg gtgcaaaatg gaatggcaga
     1441 aaacttcgtc aaaacgatga aatatgatta aatcagtgtt atgttcgaac ctgacgtgtt
     1501 aacagcagta aagtagtctc gcgaaggtgt tcaaacatta taatgaatgg catcctcata
     1561 gtgcgcaggg ttatcgcttg ctgcggaaat atctgaagcg acgtaccagt aatggggaaa
     1621 atgatagctg gtttctgaaa atatagagga aaatgtga
//
LOCUS       DAZPEB010000344         1649 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZPEB010000344 DAZPEB010000000
VERSION     DAZPEB010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.350"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(1..951)
                     /gene="corA"
                     /locus_tag="ACHURJ_004228"
     CDS             complement(1..951)
                     /gene="corA"
                     /locus_tag="ACHURJ_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFR1282916.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1103..1159)
                     /gene="ysgD"
                     /locus_tag="ACHURJ_004229"
     CDS             complement(1103..1159)
                     /gene="ysgD"
                     /locus_tag="ACHURJ_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFR1282917.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            1321..>1649
                     /locus_tag="ACHURJ_004230"
     CDS             1321..>1649
                     /locus_tag="ACHURJ_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282918.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFSVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQ"
BASE COUNT          426 a          427 c          412 g          384 t
ORIGIN      
        1 ttacaaccag ttcttccgct taaagtacag atacggtgcc aggcccgcga ggatcataaa
       61 gataatcgcg ccagggtagc cgaagctcca cttcagttct ggcataaact caaagttcat
      121 gccatagctg gaagcaacga gcgtcggcgg caggaatacc acggatacca ccgagaagat
      181 tttgatgatg cggttctgct cgatgttgat aaaacccatc gccgcctgca tcaggaagtt
      241 caccttctgg aacagagatt cgttatgcgg cagcagggat tcgatatctc gcaggatttc
      301 acgcgcctgc tccagttgtc cacccggtaa gcgtgcttta cgtaccagga agttgagcgc
      361 gcgctgggta tccatcagac acaggcgtac tttccagccg atatcttcca gttccgccag
      421 cgtggagagc gcctcatcgt actcatcgcc ctgatgccct tccataatca cccggctcaa
      481 ctgctccagg tcgctataga tattttcaat ttcatctgcc aactgttcga ttttggtttc
      541 gaacagatcc agcagcagct cgtaggcgtt accgtctacc atcgactggc tacgggcacg
      601 catacgatac agacgaaaag cgggcagttc acgctcacgc agagtaaaca gacgaccatc
      661 acggatggta aatgccacag tggagttacc ggcgtgatct tccgcatctt caaagaagaa
      721 gaaggagtga atatgcaggc cgtcgtcgtc ttcaaagaaa cgtgccgatg cttcgatgtc
      781 ttccagttcc gggcgggttg ccaggctctg gccaagttca gattgtacac gcagtcgctc
      841 gtcgtcgtcc ggttcgacaa gatcaatcca tactgcattt acaaggggtt gtgactcttc
      901 gacttccagc cgggtcagtc ggttattttc cagttgaaat gcgctcagca tgaccgggac
      961 tcccaatgct taaaatatcg gacagttcgg tgggcacaca gaaacaaatt gggtttcaga
     1021 ccattaaaca gcctgactca gcgcgacggg aaaaattgag gtcgctgaca accgctaagg
     1081 ctatcagcaa aaagggatag ccttaggagt tgatcctgga tgacaggata gtgagccagt
     1141 atctactggg tgtgtccaag gcgaatgtcc tcttagaatg atcgtgcgcg catgttacgc
     1201 cagcaaaatt ttgccgtcaa cacgcaacag aacaccacag aacaatagtt gaacttcgcg
     1261 ctttttggta ctcctcaagg tcgctaacag gataaaaaaa tcccacatcc aggacctata
     1321 atggcgcatc ggctactcat tggtaaagga atgatcacct tgaatctcaa acggattttt
     1381 ctcaccctca ccttacttcc cttgttttct gttgccgctg atgattgcgc actctcagat
     1441 ccgacgctga ccgtacaggc gtataccgtt aatcctcaaa cagagcgggt gaaaatgtac
     1501 tggcaaaaag ccaatggcga agcgtgggga acgttacatg ctctgctggc ggatattaat
     1561 agtcagggtc aggtgcagat ggcgatgaac ggcggcatct atgatgaaag ctatgcgccg
     1621 ctcggtttgt acatcgaaaa cggtcagca
//
LOCUS       DAZPEB010000345         1620 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZPEB010000345 DAZPEB010000000
VERSION     DAZPEB010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.351"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(1..1620)
                     /locus_tag="ACHURJ_004231"
                     /pseudo
     CDS             complement(1..1620)
                     /locus_tag="ACHURJ_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
BASE COUNT          420 a          411 c          349 g          440 t
ORIGIN      
        1 caaaaaggca aacagatccg aagcgggata atctttttgg ggagaattgt tccagttagc
       61 aatatatccc gacagggggt tatacacctt agggttcatt tcaaaaggca atagcccttt
      121 ccagtcccat tttcccgtac caggaacggg taatcgcgga tcatggcctg attgacgatc
      181 tggataagca ccagtatgaa cataaccaat attgccgttt acatcagcat agtaccagtt
      241 gatggtcagt gcttgtttcg ctactcctgc caatttttgg ccttcatctg atgagtccag
      301 gccagcaaag acgccacctc tttaccatcc catgcgcggg atttagcgta agccgtttgt
      361 gtcgtctggt cagtttggag aatgttgcca tgcaccgtac gccagacagt aaaggtctct
      421 gcctgaccat ttttcaccgt aatggtttcc tcacggctta acattttcac ccacttacaa
      481 ttatgtaagt agtagcctgg tttctctgcc gacagccgtt cagcaaaaat atcgacatca
      541 tcgccgaaac ctgccgttga tccccaggaa atcacaccat tatgaccaaa aaccagccca
      601 ggataggcaa atggtgtatt gccagtgaca tcataaccag caccgtgcag accaatacca
      661 taagtatacg caggcgcata ccagccaaac tgcggaccat ttaccatgat tgctttcgca
      721 tcctgggctt tgcttttgcc gatcacccac atattgctgg tcgttggata ccccgccaga
      781 ccattggcac caccctgtgc aaattgtgca gcaatagttt cccggttctt ccctgctgtt
      841 aacgccagca gtgcgccatc cgcccctttt gctggtcggt caagcattgg tgcaggtaaa
      901 tcgtagcgtg gcaacagagc tgctgtttgc gagttttgct gattaaattt aagtgggtag
      961 ctactctctt gtacggcaat agtggttggc gctgatgggt ttaccagcca tttcaactga
     1021 ttaaataccg ccatgccttg tgatacacca tatttatctt ttaaagccgt tagcagtgcc
     1081 agattatcaa tttcgctagt gctatcagag aagcggtttg ccatggtgcc cacaaatatc
     1141 atcgcgacat caaacggttc ccagcgctta ggagtaaagc caaatgtatt aaactgtttt
     1201 ggtaagagcg tctctggatt ggtatttacc ttatcaatcc aggcattcat tccatcagcg
     1261 tagccttgca gaatggacat atcctctggg gaaagggcat caatttgcgc ccggatagca
     1321 tccggccagt agttacgacg gatatcttta tcaaatttca caaaatcttt gccaagcact
     1381 tccgcgacag tcccttgagt actgcgacgt gccatttcca tctgaaaaag gcgatcttgt
     1441 gctactacat agccatagcc ataaaatagg tgccatgtat cattggcata aatatgcggc
     1501 atgccgtatt catcgcgaac aatctttatc tcacttgacg actgctcagc cagtgcaggt
     1561 aagctccaat aatacatcag ggaagcagta acacagttca cgatcatacg atttctattt
//
LOCUS       DAZPEB010000346         1619 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZPEB010000346 DAZPEB010000000
VERSION     DAZPEB010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.352"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..462
                     /gene="rfbC"
                     /locus_tag="ACHURJ_004232"
     CDS             <1..462
                     /gene="rfbC"
                     /locus_tag="ACHURJ_004232"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011093025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFR1282919.1"
                     /translation="GFFLEVYQKQRYQNLLNIEFDFVQDNYSRSSKNVLRGLHFQKTK
                     PQGKLVRVVRGEVFDVAVDIRKESPNYGQWIGVLLSEMNKKQFWVPPGFAHGFVVLSE
                     MADFEYKCTEYYDPEDECCLLWNDPELNIQWPLSNPILSDKDMKGCLLKEL"
     gene            495..1601
                     /gene="glf"
                     /locus_tag="ACHURJ_004233"
     CDS             495..1601
                     /gene="glf"
                     /locus_tag="ACHURJ_004233"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009273 - peptidoglycan-based cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HFR1282920.1"
                     /translation="MQYDFIIIGSGLFGAVCAYELNKRNKRVLVIEKRDHIGGNIYTE
                     KVDDIHIHKYGAHIFHTNDQYIWNYINKFASFNRFTNSPLAYHKGKLYNLPFNMNTFY
                     QLWGTKTPSEAQDKIDLQRSKYSNITPTNLEEQALSLVGEDVYEKLIKGYTEKQWGRS
                     CSELPPFIIKRIPVRFTFDNNYFTDRFQGVPIGGYTQIIDKMLNGVDLLLNVDYLSEK
                     DKYNSMAERIIYTGPIDAYFDKKLGTLEYRSLKFITKKIEEANYQGNAVINYTEKDIP
                     FTRIIEHKHFDDSYETEYTFITEEYPIEWSEGQEPYYPINDKKNMDIYHKYRELSQME
                     SKVIFGGRLAEYKYYDMHQVIRSALNCVNKILHD"
BASE COUNT          585 a          214 c          292 g          528 t
ORIGIN      
        1 ggtttttttt tagaagtata tcaaaaacaa cgttatcaaa atttattaaa tatagaattt
       61 gatttcgtac aagataacta ttcacgatca agcaaaaatg ttttaagagg attacatttc
      121 caaaaaacga agccacaagg taaacttgta agagttgtgc gcggcgaagt ttttgatgtt
      181 gccgtcgata ttagaaaaga atcacctaat tatggccaat ggattggtgt gttactttct
      241 gaaatgaata aaaaacaatt ttgggtacca ccaggtttcg cacatggttt tgttgtatta
      301 tctgaaatgg ccgattttga atataaatgc acagaatatt atgatcctga ggatgagtgt
      361 tgtttattat ggaatgatcc agaactcaat attcaatggc ctttaagtaa tcctatatta
      421 tcagataaag atatgaaagg ttgcttactt aaagaacttt aattttaact aagttagatg
      481 acataggaac cgcaatgcaa tatgatttca taattatagg ttcaggttta tttggtgcgg
      541 tttgcgctta tgaattaaat aaaagaaata aacgtgtgtt ggtcattgag aaaagggatc
      601 atattggagg gaatatctat actgaaaaag ttgacgatat tcacatacat aagtatggtg
      661 ctcatatatt tcatacaaac gatcaatata tctggaatta tataaataaa ttcgcttctt
      721 ttaatagatt cactaattct ccattagcat atcataaagg taaattatat aacttacctt
      781 tcaatatgaa taccttttat cagttatggg ggacaaaaac tccttctgaa gctcaagata
      841 aaatagattt acaaagaagt aaatattcta acataactcc cacaaattta gaagaacaag
      901 ctctttccct ggtaggggaa gatgtttatg aaaaactaat taaggggtat acggaaaaac
      961 aatggggacg ttcatgttct gaattaccgc cttttattat aaaaagaatc ccagttcgat
     1021 tcactttcga taataattat ttcaccgata gatttcaggg ggttcctatc ggaggataca
     1081 cacaaataat tgataagatg cttaatggtg ttgatcttct gcttaatgtt gactatttat
     1141 cagaaaagga taagtataat tctatggcgg aacggattat atatactggg cctatagatg
     1201 cttattttga taaaaagttg ggaactttag aatatagatc cttaaagttt ataacaaaaa
     1261 aaattgagga ggctaattat caaggcaatg ccgtaattaa ttatactgaa aaagatattc
     1321 cttttactag gataattgag cataagcatt ttgatgactc ttatgaaact gaatatacct
     1381 ttataactga agagtaccca atagagtgga gtgagggtca agagccttat tatcccatta
     1441 atgataaaaa aaatatggat atatatcata aatatagaga gctttcgcaa atggagagta
     1501 aagtaatttt cggaggacgc cttgctgaat ataagtatta tgatatgcac caagttatcc
     1561 gttcagcact gaattgtgta aataaaatat tacatgatta agttgttttg gcgttatta
//
LOCUS       DAZPEB010000347         1601 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZPEB010000347 DAZPEB010000000
VERSION     DAZPEB010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.353"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            196..1305
                     /locus_tag="ACHURJ_004234"
                     /pseudo
     CDS             196..1305
                     /locus_tag="ACHURJ_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
BASE COUNT          464 a          402 c          412 g          323 t
ORIGIN      
        1 tccgtaacac gggcaccatg aaaacgagtt tcactcatag cttcagcccc ttgtatccgt
       61 taaatgattc agcaacaatc atcacccacc acgcgcgtaa tctcacccct gcgccgttct
      121 cccgccacgg cgacaacaaa aagcagtaac cccctccgca cgcacatgcg accatgccgc
      181 acagggaggg agcagatgac cgacaccacc atgcaattgc tcagtcaggg cacggccccc
      241 gtgaaaatgc cggattttga tattctctcg gagggtaaaa cgctgtcagg cgtggcggag
      301 cgcctgatga gcctgtaact gaccgacaac cggggatttg aggcggacca gctcaccatc
      361 acgctggatg atgcggatgg tcagttgcag ctaccgccac ggggcgcgcg tctgacggtt
      421 ctcattggct ggaaagggga accgctgaca gaaaaaggca cttacattgt tgatgaaatc
      481 gctcacgaag gaccgccgga caggctgact gtttcagcca gaagcgcaga ttttcgggat
      541 gaatttaacg ttaaacgtga ggtgtcctgg catgatgtga ccgttgagcg tgtggtatcc
      601 gccatcactc atcggtacgg tctgaaaccg caaatcagcg aaatgctgat ggatatcgaa
      661 atcgaccacg ccgaccagac cgaagaaagc gacatgtcct ttcttacgcg catggcggaa
      721 atgctgggag caatcgccac ggtaaaaagc ggcaatctgt tattcatcat gccaggtggt
      781 ggcgtgaacg cacaggggca gccgttgccc tcgttcgcca ttacacgcag cagcggcgat
      841 cgccatcagt tccgcattgc tgaccgcgag gcgtatacgg gggtacgcgc ctactggctt
      901 gatcttaatt acgggaaaaa gaaaaaagtc agcgtgaaac gccgcaaacc gccaaaaccc
      961 aaaaaggaga aaagcagcag tcgggaaggc gactatatgg aaggtgcgga aggcaacgtg
     1021 tttgtgttac gcaagactta tcagaacgag caggcagcaa gacgcgcagc ggcggcaaag
     1081 tggcagcagc tacaacgcgg agccgcatca ttctccatca cgctggcgcg tggtcgtgca
     1141 gaactctatc ccgaaatgca tggcacagtg acaggcttta aaagcgagat tgattatcag
     1201 gactggacta ttgcaaaagc cgagcacacc attgataaca gcggctttac cacgcagctt
     1261 gaacttgaag caaaaatccc ggaatggata gcagaaacag aataaatctc ttaaaaatat
     1321 tatttttgag atgtgcttct acactgacag taagaacaaa agataatgta tcagcccact
     1381 agatgtcaga ggttggggca tcaaccagat gctaacaata atacggatgt ttttcccaca
     1441 tgacagagga agtcaagtat aatcttcaca gtccatcccg tccaagggaa tatgactcaa
     1501 tagtaaaagt tccagtttag ttagtagacc cacactttat agaagagtaa ttcatgattg
     1561 gacaattttt aagtgcaaca gagattttag caaagaacta t
//
LOCUS       DAZPEB010000348         1584 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZPEB010000348 DAZPEB010000000
VERSION     DAZPEB010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.354"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(71..493)
                     /locus_tag="ACHURJ_004235"
     CDS             complement(71..493)
                     /locus_tag="ACHURJ_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFR1282921.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELPLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSSDDSVVVH"
     gene            complement(477..704)
                     /locus_tag="ACHURJ_004236"
     CDS             complement(477..704)
                     /locus_tag="ACHURJ_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFR1282922.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            781..1188
                     /locus_tag="ACHURJ_004237"
     CDS             781..1188
                     /locus_tag="ACHURJ_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1282923.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            1391..1543
                     /locus_tag="ACHURJ_004238"
     CDS             1391..1543
                     /locus_tag="ACHURJ_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFR1282924.1"
                     /translation="MDTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LAKNTS"
BASE COUNT          445 a          414 c          328 g          397 t
ORIGIN      
        1 gccgaacctt cctccggata taatctgtga ttccccaatc aacagaacca gaggaggttc
       61 ggcataaaca tcaatgcaca acgacagaat cgtcggacga cccaccaccg ctgaaatgtg
      121 ctttccggta aacggcctga actgcgtcat catgcgcatc aattgccgta ctcaacgctt
      181 cctgcgccgc cagtaatgca cggcgttcca gggtatcgaa gatacagagt cggtgacgca
      241 gctcacgcgg aagaattgcc agaaccgcag ggatcagttt ctgaattttc tccctttgcg
      301 ctttcgtttc acctttcaac caacggtgat agatattctg ctgattgttc cagtccttgc
      361 ctggtaccag aggcaattcg ccgcccccct gccgcagata ttcttcagta attgcgttag
      421 cgacccacgc ctgccctttt tcggctgcca gggctaacag cactgattcg atgtgctcat
      481 gcctgatttt catgaatcac cttcccaggt cgttttgcct tacgatattc gtcataaact
      541 ttggggtcgt actgaagttc cccgccagat gcctcttgca ggcgcatcgc gcggccttca
      601 ggaaccagtt ccccccatgc agcaatgctt gccagcctaa ctcctgcggc attggcaagc
      661 tttgttttgc tgccaaaaaa cgctatagca tcaattttca acatatcgag ctccttagat
      721 tttcctaagg aaactagatc gtagagaaac ctaagtcaag aaagattaga attccctaat
      781 atgaaaaacg aaaccttcgg tgctcgcctc ttacataggc gtaaaaaatt aaaactgtct
      841 caggccgcat taggtaagct ggtcaaagtg gctcacgtaa caatttctca atgggaaaga
      901 gatgaaacac agccggcggg gaaaagatta ttcgcactga gccaagcgct tcagtgctca
      961 ccgacttggc ttctttttgg ggatgaagat aaacaaccag gcgaaccgat cccggataat
     1021 cagccagcca ttctgacaga agatcaaaaa gagttacttc aactgttcga cgcactgcct
     1081 gagtcagagc aaaaggccct gttgtcagag atgcgtgctc gagttgaaaa tttcaacaaa
     1141 ctttttgaag aactactcaa agctcgcaaa agaagcgcaa acaaataatc cccttttttc
     1201 tccacaccct gtaataaaaa gcacaaactt tcaaacactt gtgtttttta catcaaaaaa
     1261 acttaggttt ttatacataa aatcttgact ccacgtctta ggttattcta aatttcattc
     1321 atcaagacac cgcacggtgt tctcagcaaa cagttccact accccggcgt taaggggaaa
     1381 tgaggtcagc atggatacta tcgatcttgg caacaacgaa tccctagtgt gtggcgtgtt
     1441 tcccaatcag gacggcacat tcaccgccat gacgtatacc aaaagcaaaa cgtttaaaac
     1501 cgaagctggc gcacgtcgct ggcttgccaa aaataccagc taatccattt attggattaa
     1561 ttcaatattc tcgctgtaga ggta
//
LOCUS       DAZPEB010000349         1574 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZPEB010000349 DAZPEB010000000
VERSION     DAZPEB010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.355"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..91
                     /locus_tag="ACHURJ_004239"
     CDS             <1..91
                     /locus_tag="ACHURJ_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFR1282925.1"
                     /translation="GIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            94..168
                     /gene="yoaJ"
                     /locus_tag="ACHURJ_004240"
     CDS             94..168
                     /gene="yoaJ"
                     /locus_tag="ACHURJ_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFR1282926.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(227..475)
                     /locus_tag="ACHURJ_004241"
     CDS             complement(227..475)
                     /locus_tag="ACHURJ_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFR1282927.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(623..805)
                     /locus_tag="ACHURJ_004242"
     CDS             complement(623..805)
                     /locus_tag="ACHURJ_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFR1282928.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(809..1169)
                     /gene="yeaR"
                     /locus_tag="ACHURJ_004243"
                     /pseudo
     CDS             complement(809..1169)
                     /gene="yeaR"
                     /locus_tag="ACHURJ_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
     gene            complement(1342..>1574)
                     /locus_tag="ACHURJ_004244"
     CDS             complement(1342..>1574)
                     /locus_tag="ACHURJ_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFR1282929.1"
                     /translation="FFVQFIDVNAPHTGISFFILATTLELVSFCYLSFLIISGAFVTQ
                     YIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
BASE COUNT          432 a          375 c          350 g          417 t
ORIGIN      
        1 cggtattatt tttccggttg taatctttat tacagcggtc gtatttttag catggttttt
       61 tattggcggc tatgctgccc cgggggcata aagatgaaaa aaacaacgat tattatgatg
      121 ggtgtggcga ttattgtcgt actcggcact gagctgggat ggtggtaacg tcacctctaa
      181 aaaatagcaa aggctgcctg tgtgcagcct ttgtgcaatt taagcgttaa cttttaatct
      241 tcctgtagat aaatagcacg acaatcgcac caataacggc aaccacgaag ctgccaaaat
      301 tgaagccatc gactttacca aagccaaaca gcgtgctgat ccatccgccg actacggcac
      361 cgactatccc cagcaggata gtcataaaga atccaccacc atctttacct ggcatgatcc
      421 acttcgccag aataccggca ataagcccaa aaataatcca tgacagaatg cccattgttt
      481 cctcacttat ctgttttgca ttagcgggtt agtcgctgat aaaaagcata gcacaacatc
      541 gggagggcaa gatttgtgac gagcatcacg gaggattttt ttgcgatggc gcagaaattg
      601 cgccatcaac gatcagtgat aattaccaac cacaaacatc atgttcgttt tccgtgtcat
      661 aagaacgcac ggtattcacc agatctttta tcacttcagc cgccacttct ggcaccagca
      721 aagtcatcgg cgtctctgtt tcataatcga cagaaacgcc attactgtta ttggtgacgg
      781 tcacggtata cgttgctttg cccatgattc atttcccgtt atgaatgact ttccgttgtt
      841 gcgcaccttc catcaggact tcaggagcca cgaagaagtc aatgttgaca taagtatcgt
      901 cagtcatggc ttcaatgttg tgccactttt ctggagggaa caccgcaaac tgccccgctt
      961 cgataaggat cacctgatca ggctctgcac tgtgttcatc agcgtagccg agatatttga
     1021 ccgccccatg cataacggaa aggcgtgggt aacccccctg gcgcgttcct ttatcaagat
     1081 gacgttcgaa ttattccggc aggcgcagtt tgtttattcc agaaaggcgt tgagcgcgta
     1141 tgaatataat tctgtgggat ttgcagcatc cttttccctc cttcggtgaa tgcgctgaaa
     1201 acggtttatt ccagccgttt cagggtacgc ctgataattt gcattttaaa taccatttat
     1261 tggttacttt ttagcaccat atcagagaaa aatcagggag gattacaagt gggaagcccg
     1321 cgacaacgcg ggctgaatgt atcaggattg cagcgtcgcc agtcgggcgg cgaaacccac
     1381 gaacatcaaa ccaatcagtg agttgccaac tttagccagt ttctttttgg tacgtatgta
     1441 ctgcgtgaca aaagcaccag aaataatcag gaagctcaaa tagcagaaac tcaccagttc
     1501 cagcgtcgtc gccagaataa agaatgaaat tcccgtatgt ggggcattaa catcgataaa
     1561 ctgtacgaaa aacg
//
LOCUS       DAZPEB010000350         1557 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZPEB010000350 DAZPEB010000000
VERSION     DAZPEB010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.356"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(38..316)
                     /locus_tag="ACHURJ_004245"
     CDS             complement(38..316)
                     /locus_tag="ACHURJ_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFR1282930.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSANVSAPAQST"
     gene            424..1134
                     /locus_tag="ACHURJ_004246"
     CDS             424..1134
                     /locus_tag="ACHURJ_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFR1282931.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSDEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYESHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            complement(1137..>1557)
                     /gene="speG"
                     /locus_tag="ACHURJ_004247"
     CDS             complement(1137..>1557)
                     /gene="speG"
                     /locus_tag="ACHURJ_004247"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HFR1282932.1"
                     /translation="TTSHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIIS
                     PEYQGKGLATRAAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFTVEGELMHE
                     FFINGQYRNAIRMCIFQHQYLAEHKTPGQTLLKPTAQ"
BASE COUNT          426 a          337 c          384 g          410 t
ORIGIN      
        1 cgataatgta ttactgcggt tcggcaggcg cgccatccta ggtagactgc gcgggagcag
       61 agacgttagc gctggtggtg cgggtgtaga gaattttatg cgtatcatta gcacaatgcc
      121 cgacgacctg ggaatcaggc tgatcaacct ggtcattggg tacaatactt aatgtgaagc
      181 tgctttcggg tacgccatta ttgataatgc gctgtgatat atcgctctgt atgcgctcac
      241 aggatcccgg cgcggcgagt accgcgggtg aggcgagggc gagcagaagc gcggcacagc
      301 aggttgagag tttcatcata agctccttac gcgaagataa cttctttaag catagcattt
      361 aacgtgtaaa gtactgtatt tgctactatg attgagaatc atctctactc tctggtgact
      421 gttgtgaaat acaaattact accatgctta ctcgcgatat tcctcacagg atgtgaccgc
      481 acagaggtaa cactttcatt tacccctgag atggccagtt tctctgatga attcgatttt
      541 gatccgctgc gtggtccggt aaaagatttc actcagacat taatggatga gcaaggtgaa
      601 gtgacgaaac gtgtttctgg gactttgtcg gaagaaggct gttttgattc actcgaatta
      661 ctggatctgg aaaataatac cgtggtcgct ctggtactgg acgccaatta ttaccgtgat
      721 gccgagacgc tggagaagag agtacgttta cagggaaaat gccagctagc agaattacct
      781 tctgccgggg tgagttggga aaccgatgat aatggcttcg tgattaaagc cagcagcaaa
      841 caaatgcaga tggaatatcg ctatgatgat cagggttatc cactgggtaa aaccacgaaa
      901 agtaacgaca aaacattatc tgtcagcgcc acgccatcaa cggacccgat caaaaaatta
      961 gattacacag cggttacttt actgaataat caacgagttg gtaatgtaaa acagagctgt
     1021 gaatatgaga gtcacgccaa tccggtggac tgtcagctga tcattgttga tgaaggagta
     1081 aaacccgccg tcgaacgggt ttacaccatc aaaaatacga tcgattatta ttaatgctat
     1141 tgtgcggtcg gcttcaggag agtctgaccc ggtgttttgt gctctgccag atactgatgc
     1201 tggaatatac acatgcgaat ggcattacga tattgaccat taataaaaaa ctcatgcatc
     1261 aattcacctt caaccgtaaa gccaagcttg cggtaaatgt gaatcgcttt ttcattctct
     1321 ttatcgacga tcagatacag cttatagaga ttgagaacgg tgaagccata gtccattgct
     1381 aatttggcgg cacgggttgc cagacctttc ccctgatact ccggggagat aattatctga
     1441 aattctgccc ggcgatgtac atggttaatt tccaccagct ccaccagacc ggctttttcg
     1501 ccgtcacatt ccaccacaaa gcgtcgttcg ctctgatcgt gaatatgtga agtggtc
//
LOCUS       DAZPEB010000351         1546 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZPEB010000351 DAZPEB010000000
VERSION     DAZPEB010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.357"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..>1546
                     /locus_tag="ACHURJ_004248"
     CDS             <1..>1546
                     /locus_tag="ACHURJ_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFR1282933.1"
                     /translation="IRENMSEFARCHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQ
                     PEDLCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIR
                     AKISKDIESGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKDNISKDE
                     WENLHALKEKRLNGMPKTTPKSGQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIE
                     RSITPPLKNYNERLHNTNLFNPKYQKKAVMNYMEYLVTCQLDEQQMSSLLYERDDLSA
                     IDFSLLMKTIKAFSFGGDLQTLASKPGSTISSIPSERRILININHDFPNNGNLFNDFL
                     FNHQQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWLNKGCST
                     YQQLYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNE
                     EVEGTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPGKVFTLRGGTLNINIPGRSALL
                     LGKTGEP"
BASE COUNT          534 a          294 c          302 g          416 t
ORIGIN      
        1 atcagggaaa atatgtctga gtttgcacgt tgccattatg ggtatataca aatccctcca
       61 gtgacaactt tccgcgccga cggaccagaa actcccgaag aagaaaaagg ttactggttt
      121 catgcttatc aacccgaaga tctttgtacc atccataacc caatgggaga tttgcaggat
      181 tttatcgcat tggttaaaga tgctaaaaaa tttggtatcg atatcattcc tgattataca
      241 tttaacttta tgggaatcgg gggtagtggt aaaaatgacc tggattatcc ctctgctgat
      301 atacgagcga agatcagtaa agatatagaa agcggtatcc ctggctattg gcaaggtcag
      361 gttttgattc cattcattaa agatccagta acaaaagaac gtaaacaaat ccatccagaa
      421 gatatacatc tcactgcaaa agacttcgaa gcaagtaaag ataacatctc taaggatgaa
      481 tgggaaaacc tccatgcatt aaaagaaaag cgtttaaatg gaatgcctaa aacaacaccc
      541 aaaagtggcc aggttattat gttgcaaaat caatacgttc gagaaatgcg aaaatatggc
      601 gtacgaggtt tacgttatga tgcggcaaaa cactcaaaac atgaacaaat agagaggtcg
      661 ataaccccac cgcttaaaaa ttataatgag cggttacaca atactaattt atttaaccca
      721 aaatatcaaa aaaaagccgt tatgaattac atggaatacc tggtaacttg tcagttggat
      781 gaacaacaaa tgtcatcact gctttatgag agagatgatt taagtgccat tgatttttct
      841 ttactcatga aaacgataaa agccttttca tttggtggag atctccaaac ccttgcatca
      901 aaaccgggtt ccacaatctc aagtatccca tcagaaagac ggatattgat taacattaac
      961 cacgattttc ctaacaatgg taatcttttc aatgactttc tatttaacca tcaacaagat
     1021 gaacaattag caatggcata tatggccgct ctcccgttca gcagaccttt agtttactgg
     1081 gatggccaag tattaaaatc aacgactgaa attaaaaatt atgatgggtc cacgcgtgtc
     1141 ggcggtgagg cgtggcttaa taaaggttgc tctacctatc agcagctcta caatgaattc
     1201 cacgcattat atatagataa agcaggaata tggagcgcat ttgagggtgt atttgcaact
     1261 aaaaacgttc tggcctttag tcgtggggat tctgtgaaca ttaatcactc tcctcatgat
     1321 gggctagtta taataaataa aggaaacgaa gaagttgaag gtacctggcc taacaaattg
     1381 caacctggaa tatacaaaaa catggggagt aatagcgtta acattattat taataatacc
     1441 cgaaaaatta tcccccctgg taaagtattt acgcttagag gcggaactct aaatatcaat
     1501 attcctgggc gtagcgctct tcttttaggg aaaacaggag aaccgc
//
LOCUS       DAZPEB010000352         1496 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZPEB010000352 DAZPEB010000000
VERSION     DAZPEB010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1496
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.358"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..1494
                     /gene="pdeB"
                     /locus_tag="ACHURJ_004249"
     CDS             <1..1494
                     /gene="pdeB"
                     /locus_tag="ACHURJ_004249"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFR1282934.1"
                     /translation="LPVGLSIWLAHQQVETSFIEELDTYSSRVAIRANKVATQGKDAL
                     QELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNVPQCSSLEHESPPDTFPEPG
                     KISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPASFIDVIPYSSWQIDAAIIGN
                     AHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILPFPEMNISIITWASTKMLQK
                     GWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQDAIENRDICVHYQPIVSLA
                     NGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPLTLLIIRSVFEDMGDWLRQH
                     PQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIALELTEREFADPKTSAPIISR
                     YREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVDALEYKNVTPHIIEMAKTLK
                     LKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDFLRWAEQHL"
BASE COUNT          396 a          351 c          367 g          382 t
ORIGIN      
        1 ctgcctgtcg gcttaagcat ctggctggcc catcagcagg tagaaacatc gtttattgaa
       61 gagctggata cctattcctc ccgcgtcgct attcgggcca ataaggtggc gacacaaggg
      121 aaagatgcgc tgcaagagct ggaaagatgg caaggcgctg cctgtagcga agcccatctc
      181 atggaaatgc gtcgggtatc ttacagttat cgctatattc aggaagtggt ttatatcgat
      241 aacaacgttc cccagtgttc gtctctggag catgaaagtc cgcccgatac cttccccgag
      301 ccaggtaaaa tttcgaaaga tggttatcgt gtctggttaa catcgcataa cgatttaggc
      361 attatccgtt acatggtcgc catgggaacg gcacattatg tcgtcatgat cgaccccgct
      421 tcctttattg atgtcattcc ctatagctca tggcaaattg atgccgccat tattggcaat
      481 gcccataacg ttgtcataac cagcagcgat gaaattgctc agggaattat taccaggcta
      541 caaaaaacac ccggtgagca tatcgaaaat aatggaatca tttacgatat cctgcccttc
      601 ccggagatga atatttcgat catcacctgg gcttcaacga aaatgttgca gaaaggctgg
      661 catcggcaag tctttatttg gttaccgctc gggttggtga ttggcctgct ggcagcgatg
      721 tttgtgctgc gtattttgcg ccgtatccag tcaccgcatc atcggctgca ggatgctatc
      781 gaaaatcgtg atatttgcgt gcactatcag ccgattgtct ccttagccaa tggcaaaatt
      841 gtcggtgctg aggcactggc gcgctggccg cagacagacg gtagttggtt gtcaccagat
      901 agttttattc cgctggcaca gcaaacaggc ctttctgagc cattgacgct actgattata
      961 agaagcgtct ttgaagatat gggcgactgg ctgcgtcagc atccacagca gcatatttcg
     1021 atcaatcttg aatccaccgt gctcacctcg gaaaaaatcc cgcaattgct gcgtgaaatg
     1081 atcaatcact atcaggttaa tcccagacag atcgcgcttg aactcactga acgcgagttt
     1141 gccgatccga aaaccagcgc cccgataatt tctcgctacc gggaggcggg ccatgaaatt
     1201 tatcttgatg attttggtac gggatattca agtttaagtt atttacagga tctggatgtc
     1261 gacattctga agatcgataa atctttcgtt gatgcgctgg aatataaaaa tgtcacgccg
     1321 catatcatcg aaatggcaaa aacactgaaa ctgaaaatgg tagcggaggg aatcgaaacc
     1381 agtaaacaag aagagtggct acgccagcat ggcgtgcact acggtcaggg ctggctctac
     1441 agcaaggcat taccgaaaga agatttctta cgctgggccg agcaacattt gtgaat
//
LOCUS       DAZPEB010000353         1459 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZPEB010000353 DAZPEB010000000
VERSION     DAZPEB010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.359"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..730)
                     /gene="pdeC"
                     /locus_tag="ACHURJ_004250"
     CDS             complement(<1..730)
                     /gene="pdeC"
                     /locus_tag="ACHURJ_004250"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFR1282935.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHL"
     gene            1160..1441
                     /locus_tag="ACHURJ_004251"
     CDS             1160..1441
                     /locus_tag="ACHURJ_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFR1282936.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT          421 a          321 c          324 g          393 t
ORIGIN      
        1 taagatgatt ataaaaatgg gttataagac gttgatatga agtcgcaaca atggctgcaa
       61 ttgggcgttt tgtcgaataa actgtcgcat ataaatagcc atttctttgt acggttaaat
      121 ccttcaaatg aatcagcgga gaaaacgttg ctgccgaggc ctctttgctt aacgagaaaa
      181 aggttttcgt caccgtatca tacacacccc attgcaatgt cggatcatca gacatcactt
      241 cactccagaa gagagggttg ataaccgcca cataatttcc ccgctgcata taggtcattt
      301 tatagccaga gaaaaaaggc gtatcgcggt aataatagat agaaacgtta ggttcacgct
      361 tataatcggc cggtgcaatc gtatagccgt ttacaggcgc tatcagcgat gagcataaaa
      421 aatggttatc acgggcatag atcaattcat taatataaag atagccacga ataatattca
      481 acattcgctt ttgatgggct ggagtgcaaa cttgcccctg atagcgttca gctgcatcgc
      541 tcactaaatc tgcctggcga atgaccagct cggatttatc cagtgccagt tgagcaaagg
      601 ttcggagctg attattcact tctgattttg cccaaaggaa ggcaatccat agcgaaagaa
      661 tgatgggaaa gagaactaaa aagataatgc ccggcaacgc cagtaattgg tgtcgtgcac
      721 gatgactcat tgctatcaat ttcctgcctg tttatacccg gccattgtaa ggtgaaaacc
      781 ctgggtaccg acgtctttag ataaatctta cggtataaat agcacttttg cctgatttga
      841 aattgatacc tttctaatcg tcacctggat gaaaataatt cattgatatt caaaattaat
      901 ttcctacagg aatgcagagg cggcgggaag atatgccctt aatgccactg aaaaaccaca
      961 taatctgtac agcaataaaa gtcacggcct aatatggcta acaaacaatc aattgtgata
     1021 tatttcacaa aattaatgaa acaaacagag tgtttcattt ttgtgttctg caagccccgc
     1081 gatagccaga gtgtaccacg cctcccgtga acaacgccgc gctgtccagg gcatcggctc
     1141 ttttcagagg aattgtttga tggctaccct caccactggc gtggttcttc ttcgctggca
     1201 acttcttagt gccgtaatga tgtttctggc cagcacactc aacatccgtt ttcgtcggtc
     1261 tgattatgtc gggcttgcgg tgatcagcag cggtctgggc gttgtttccg cctgttggtt
     1321 cgcaatgggg ttgcttggca tcacaatggc ggacatcacc gctatctggc acaacatcga
     1381 gtcggtgatg atagaagaga tgaatcagac accgccacaa tggccaatga ttttgacctg
     1441 atacgtagaa gcctccata
//
LOCUS       DAZPEB010000354         1454 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZPEB010000354 DAZPEB010000000
VERSION     DAZPEB010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.360"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..81)
                     /locus_tag="ACHURJ_004252"
     CDS             complement(<1..81)
                     /locus_tag="ACHURJ_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282937.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANN"
     gene            674..892
                     /gene="ccdA"
                     /locus_tag="ACHURJ_004253"
     CDS             674..892
                     /gene="ccdA"
                     /locus_tag="ACHURJ_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HFR1282938.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
     gene            894..1199
                     /gene="ccdB"
                     /locus_tag="ACHURJ_004254"
     CDS             894..1199
                     /gene="ccdB"
                     /locus_tag="ACHURJ_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HFR1282939.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            1235..1330
                     /locus_tag="ACHURJ_004255"
                     /pseudo
     CDS             1235..1330
                     /locus_tag="ACHURJ_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
BASE COUNT          396 a          338 c          355 g          365 t
ORIGIN      
        1 attattggct ctggcgacgg ctttcagaac ctctttcacc attctggtgt ggactttctg
       61 gtgctccagg ttgtgtgaca tgggaactca ttctggatgg ttactctgaa agctcatatt
      121 ctgccacacc ccgatttgca gccgccaggc tgccgtggtt cgtgaaccgg gctataacct
      181 gcagtcggtt gttgaacgcc tgacaacagg aaatcagcca ggccagcaac cacgcggact
      241 gacagagaat cagaacctga atcagcctga aatcataccc ggggcagtac ttcatcacca
      301 gtcaccaata cccgtgtcag tacttcgcgg cctgacgcga tcacccgact cagtacttct
      361 gagcccgcct ccggtaccat tctctgtggg cccgtacaca acaacagaaa agggtgcccg
      421 gcccggggcg caaccgtatt acacttccgg aaaatcgtta tacgcagaaa aatcggatca
      481 cgctgtgcct gctttcatgt aatttactta cggtgttata agtatgctaa ttatcgtata
      541 aaaaaatcgc agcagaaaaa attgtattaa aaacttatag tgaattaatg gtgtactttt
      601 tatggatatt gatggtttgt accattaata tatactcata tacatatcat aagtatgttt
      661 ttgagggctt atcatgaagc agcgtattac tgtcactata gacagtgaca gctatcagtt
      721 acttaaatcc gcaaacgtga atatctcagg cctggtaaat acggccatgc aaaaagaggc
      781 ccgccgtctg cgtgcggaac gctggcaggc tgaaaatcag caggggatgg ctgagatcgc
      841 ccgttttatt gaaatgaacg gctcttttgc cgatgagaac agggactggt gaaatgcagt
      901 ttaaggttta cgcctataaa agagagagtc gttatcgtct gtttgtggat gtacagagtg
      961 acattattga cacgcccgga cgacggatgg tgatccccct ggccagtgcc cgcctgctgt
     1021 cagataaagt ctcccgtgag ctttacccgg tggtgcatgt cggggatgaa agctggcgca
     1081 tgatgaccac tgatatggcc agtgtgccga tcttcgttat cggggaagaa gtggctgatc
     1141 tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg ggaatataaa
     1201 tgtcaggcta gggtcaaaaa tcgtggcgtt gacaaaatgg ctgcgttacg tcattgagca
     1261 tatccaagac tggccggcaa accgggtacg cgatctgttg ccctggaaag ttgatctgac
     1321 ctctcagtaa atatcaatac ggttctgacg agccgcttac cgttcaaata tgaagtacga
     1381 tgtttaacta accgaaaaac aagaacaata cggtgcaaac aggccattca cggttaactg
     1441 aaacagtatc gttt
//
LOCUS       DAZPEB010000355         1441 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZPEB010000355 DAZPEB010000000
VERSION     DAZPEB010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.361"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..465)
                     /gene="virK"
                     /locus_tag="ACHURJ_004256"
     CDS             complement(<1..465)
                     /gene="virK"
                     /locus_tag="ACHURJ_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HFR1282940.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLPCIEDILAIHPTLPARIHRPYLHKGGRAWTRGQYILEHYRFVQNL
                     PEKYSKFLFPQKSVSLVQFIGKDGENFDIQCSSSGFDREGELMLSLFYNKTVI"
     gene            complement(470..>1441)
                     /gene="capU"
                     /locus_tag="ACHURJ_004257"
     CDS             complement(470..>1441)
                     /gene="capU"
                     /locus_tag="ACHURJ_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HFR1282941.1"
                     /translation="NSKIAFEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAI
                     VCHSGHDSNIVGLVRLFTWKHPFRIIRQKTYLTQKTKVFSINHFCDEVIVPGTSMKTH
                     LEQEGCRTRVTVVPPGFDFQKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKG
                     HEFMLNLLFHLKMNGRQFCWLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAP
                     VYRVASLVVLPSENESFGMVLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKH
                     AWMCALNDFFNDPGRFYQMARQAKQDIEERFDINKTALKILTLAKHK"
BASE COUNT          445 a          329 c          275 g          392 t
ORIGIN      
        1 aataactgtt ttattgtaaa acaatgacaa catcaactct ccttcccggt caaaaccact
       61 ggaagaacac tggatatcaa aattctctcc atctttcccg atgaattgga ctaatgatac
      121 cgatttttgt ggaaacaaaa atttagaata tttttctggc aaattttgca caaagcgata
      181 atgctcaaga atatattgtc cacgggtcca ggcgcgcccc cctttatgca aataagggcg
      241 atgaattctt gctggcaacg tggggtgtat tgccagaata tcctcaatgc acggcaagga
      301 acgcagttcg taataatatc gaatactggc aaccggattt atagcacatc gcaaaatgta
      361 catgaaccgg aattttctgt gttcccactt ccccgggagt gaatctgagg aaaaaacaat
      421 ccttttaagg aaaccgataa atgataagtt acttacagaa aacatattgt tacttgtgct
      481 tcgctaatgt gagtattttt aacgcagttt tattaatatc aaaccgctct tctatatcct
      541 gttttgcctg gcgagccatc tgataaaaac gcccagggtc attaaaaaaa tcattcaggg
      601 cgcacatcca tgcgtgctta ttacctgctg gtaacaatgt cccggtctgg ttgttctgaa
      661 taacatcagg gattccacca atctgactgg ccagtacagg cacagaaaat gccgatgctt
      721 ctgccagtac cataccaaac gattcgtttt ctgaaggcag aaccaccaga ctggcaaccc
      781 gatatacggg ggcggcagga aaaacattgt cagcaataaa aacatcatca tgcatgccca
      841 tactgtcaat ctgatactgt aaatgctccc gcagttcagg cgaaccagac cccacaatca
      901 accaacagaa ctgtcgtcca ttcattttta aatggaacag taaattcaac ataaattcgt
      961 gccctttttc cgggcgcaac attcctacct gagcaataac ggggcatccc cttcgggacg
     1021 ccagccaaga aagaacattt ggaggcaaac tgtttcggga atcaacgtat aatttctgga
     1081 aatcaaagcc tggaggcaca acagtaaccc gggttcgaca tccttcctgc tccagatgtg
     1141 tcttcatact tgttccggga acaatcacct catcgcaaaa atgatttatt gaaaaaactt
     1201 ttgttttttg tgtcaaatat gtcttttgcc tgataattct gaatggatgt ttccaagtga
     1261 ataaccgtac taaaccaaca atattgctat catgcccact gtgacaaacg attgcattgg
     1321 gctgaaaacc atgaactatt ccgagtaatc tccatgcagt agggatgtga agactgtttc
     1381 gaaataacgc gaatgtgata tcaattccca atttactggc ttcaaaagca attttgctgt
     1441 t
//
LOCUS       DAZPEB010000356         1416 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZPEB010000356 DAZPEB010000000
VERSION     DAZPEB010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.362"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(415..1344)
                     /gene="virB"
                     /locus_tag="ACHURJ_004258"
     CDS             complement(415..1344)
                     /gene="virB"
                     /locus_tag="ACHURJ_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HFR1282942.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
BASE COUNT          389 a          232 c          256 g          539 t
ORIGIN      
        1 cttaaagctt ttttgatact gtgaacgaag tctgttaatg gtggattttt tgtttatcac
       61 catgtaaaaa aattgagtag taaaagcgtt gactgctaat cctcgctgtt tttattgaaa
      121 cgattaaaaa tacgctgaat attttcggcc agtcactcat cttcacataa aattttgacc
      181 atcatgccgc atcccttagt tgcacaattc attttcaatg atatgaatat tactaatttc
      241 accagataat gttctcgtgg tcagcagggt atgagttttc accgtaattt taaggaaaca
      301 cagtgcgtgg ggattgaata acagatagcc gacggaatta ccccaccggc tgaaagtcaa
      361 gttcaacgaa gatttttatt atctgaattg ggcagtttac atcagtgttc gagtttatga
      421 agacgataga tggcgagaaa ttatatctcg aatagcttca tctatttctc tctgaactgt
      481 tttgtttatt ttagataatg taaatattag agttctgttt gttttattct cttttctttt
      541 tatgtaagtc cgtttgtctt tagaaataaa taacgagtca acttttagcg aagggttttg
      601 ctttctgttt ttgcttttct ttatgatgtt taaaatttct tttttatata tgtccggggg
      661 caaatttgta tcaaggtctt ttatttcttc ctttaatatt ggtagtgtag aactaagaga
      721 ttcattagcc ttttcaagtc ctttataata attgaataat atcttgtagt cattaaagtt
      781 aagttctgac gcgattggaa atagagatat tatttcctgt ggaacgcttg ctgcctgaaa
      841 ggcacgagtg actttcgcgc gagacagatt ctcttttttg gctatgtctt tataggacat
      901 ccctgatact ttcagaaaat taagaccaat accaagttct cggatgctat gctcttttgc
      961 tgtttgtata tcgtttgcta gttttctggc atcaagagta gatatatatt cttttgaata
     1021 tagaacttca agatctgctc ctgcatatat tgcagatgct cttctacgag tgccatccag
     1081 aatttcaatt ctaccatcaa tctcccttcc tattacaggg aagaattgtt gtagctttat
     1141 agtttttatg atatcggcta atgattcttc tgttagggat tcttgatcgc gtacattcgt
     1201 tttttggttg acgaaggtta aatcttggat ccttttatga ggaatcttgg ctttgataaa
     1261 ggaaacttta ttaccagaat ggagtgtaaa ctctttcttt tggccttcct ttatacttaa
     1321 caagtcgttg cacaaatcca ccatatcaca ccctgtttat tcatattgat ttatattgac
     1381 aaaagttaaa tgcagtgtga gcaagattgt atgtaa
//
LOCUS       DAZPEB010000357         1390 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZPEB010000357 DAZPEB010000000
VERSION     DAZPEB010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.363"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            7..150
                     /locus_tag="ACHURJ_004259"
                     /pseudo
     CDS             7..150
                     /locus_tag="ACHURJ_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449236.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            542..>1390
                     /locus_tag="ACHURJ_004260"
     CDS             542..>1390
                     /locus_tag="ACHURJ_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR1282943.1"
                     /translation="MIKSTNIQAIGSGIMHQINNIYSLTPFPLPMELTPSCNEFYLKA
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDSIPPFPDKLEGLAMANNFIEQLPELPFSMNRAVL
                     MNNNLTTLPESVLRLAQNAFVNVAGNPLSGHTMRTLQQITTGPDYSGPRIFFSMGNSA
                     TISAPEH"
BASE COUNT          493 a          281 c          229 g          387 t
ORIGIN      
        1 taaacaaaaa cagccatata cgaattgcaa atacctgcta ccaaccgaac gaaaaagttg
       61 aaattcacag ctacagaaat acaatggttg acaaagataa atgaagccgg aattaatgaa
      121 aaacaaagcc aacggtattc tgatttctga agttaatttc agactcgttt aagctccatt
      181 ttcaaaaaaa atatatcagg gtttgttact aataacttct gaaaacagaa aaaaaataga
      241 gcagtaattc tatttcagag aggagtaact ccatctatcc tcgatacgta ctgaacgtta
      301 tcgtgatata ttcatcatgc tttcttcacc attattcgag tacagggaga gacgcaatga
      361 ccttaaatac ctttttatgg ctgaattacc attctcagga ataacagcag actccttccc
      421 atagacggct tagggggaaa gcaatacccg gagaaagggt acaaagcaaa ctgttttaaa
      481 attaataagc tatattctat ttcactagaa ttcttatcgc tgataagaaa ttttagttat
      541 aatgattaaa tcaaccaata tacaggcaat cggttctggt attatgcatc aaataaacaa
      601 tatatactcg ttaactccat ttcctttacc tatggaactg actccatctt gtaatgaatt
      661 ttatttaaaa gcctggagtg aatgggaaaa gaacggtacc ccaggcgagc aacgcaatat
      721 cgccttcaat aggctgaaaa tatgtttaca aaatcaagag gcagaattaa atttatctga
      781 gttagattta aaaacattac cagatttacc gcctcagata acaacactgg aaataagaaa
      841 aaacctatta acacatctcc ctgatttacc accaatgctt aaggtaatac atgctcaatt
      901 taatcaactg gaaagcttac ctgccttacc cgagacgtta gaagagctta atgcgggtga
      961 taacaagata aaagaattac catttcttcc tgaaaatcta actcatttac gggttcataa
     1021 taaccgattg catattctgc cactattgcc accggaacta aaattactgg tagtttctgg
     1081 aaacagatta gacagcattc ccccctttcc agataagctt gaagggctgg ctatggctaa
     1141 taattttata gaacaactac cggaattacc ttttagtatg aacagggctg tgctaatgaa
     1201 taataatctg acaacacttc cggaaagtgt cctgagatta gctcagaatg ccttcgtaaa
     1261 tgttgcaggt aatccactgt ctggccatac catgcgtaca ctacaacaaa taaccaccgg
     1321 accagattat tctggtcctc gaatattttt ctctatggga aattctgcca caatttccgc
     1381 tccagaacac
//
LOCUS       DAZPEB010000358         1345 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZPEB010000358 DAZPEB010000000
VERSION     DAZPEB010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.364"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            87..1006
                     /locus_tag="ACHURJ_004261"
                     /pseudo
     CDS             87..1006
                     /locus_tag="ACHURJ_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            1006..>1345
                     /locus_tag="ACHURJ_004262"
     CDS             1006..>1345
                     /locus_tag="ACHURJ_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR1282944.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYLVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVNAERIGVLG"
BASE COUNT          381 a          302 c          327 g          335 t
ORIGIN      
        1 atttagggtc taatcaactt aatgatcaat atctatagtg atatcaccaa ccgttaaaac
       61 gaaccgacaa ctccatgagg aacggaatga tgaataataa agtcagcttc actaacagca
      121 ataatccaac catctctttg tctgcagtga tctatttccc tccaaaattt gatgaaaccc
      181 gtcagtatca ggcaattgtg gtatctcacc caggtggggc gttaaagaac aaacggccgg
      241 aacctatgcc aaaaaactgg cggaaaaggg atttgtcact attgcttatg acgcatctta
      301 tcaaggtgaa agtggcggcg agccgcgtca gttagaaaac ccatacattc gtactgaaga
      361 catcagtgcc gtgattgatt acctgacaac gctttcttat gtcgacaata cccgaattgg
      421 tgcgatggga atttgtgccg gtgcaggata tacagccaat gctgcaattc aggatcgccg
      481 catcaaggcc atcggtaccg ttagtgcggt caatattggt tcgatgttcc gtaatggttg
      541 ggaaaacaat gtgaaatcta ttgatgccct gccatacgtt gaagcgggtt caaacgcccg
      601 aaccagcgat attagcagcg gagaatatgc cgttatgcca ctggcaccaa tgaaggagtc
      661 cgatgcgccg aacgaagaac tgcgtcaggc ctgggagtat taccacaccc ctcgcgcgca
      721 gtatccaaca gcaccgggtt acgctacttt gcgcagcctt aaccagatta ttacctttga
      781 tgcttaccat atggcggaag tgtacctgac tcagccgatg cagattgtgg cgggcagcca
      841 ggcagggagc aaatggatga gtgacgatct gtacgatcga gcatcaagcc aagataaacg
      901 ctatcacatt gttgaaggcg caaaccatat ggatctttat gacggcaaag cctatgtggc
      961 tgaagcaatt tcagtattag ctccgttctt tgaggaaact ctgtaatgaa acatattcaa
     1021 atccgcaata gtgatatggc ctggcatatt gctgccaata ttcagtttcc accaaatttt
     1081 gatgagtcaa aacagtatcc ggcgattatc agcgtccatc cttttggcag ttgtaaggag
     1141 cagacgtctg gcaatatcta tggcaaagca ttggctgaaa aaggctatct cgttctcgct
     1201 tatgacgcca gtttccaggg agaatctggt ggggagcctc gctggataga agatccgaca
     1261 cagcgcgtag aggatattag ccgtgttatc gattatgcag taactctgcc atatgttaat
     1321 gctgaacgta ttggcgtgct gggag
//
LOCUS       DAZPEB010000359         1342 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZPEB010000359 DAZPEB010000000
VERSION     DAZPEB010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.365"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            80..259
                     /locus_tag="ACHURJ_004263"
                     /pseudo
     CDS             80..259
                     /locus_tag="ACHURJ_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(276..965)
                     /locus_tag="ACHURJ_004264"
                     /pseudo
     CDS             complement(276..965)
                     /locus_tag="ACHURJ_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1044..1136
                     /locus_tag="ACHURJ_004265"
                     /pseudo
     CDS             1044..1136
                     /locus_tag="ACHURJ_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1159..>1342)
                     /locus_tag="ACHURJ_004266"
     CDS             complement(1159..>1342)
                     /locus_tag="ACHURJ_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HFR1282945.1"
                     /translation="IYQPVYSPWVNHVERLWQALHDTITRNHQCRSMWQLLKKVRHFM
                     ETVSPFPGGKHGLAKV"
BASE COUNT          360 a          302 c          333 g          347 t
ORIGIN      
        1 atcatatttt agccaagctc atttattcct attatagaat gataaattaa aattactgta
       61 aaaaaacgat acacatttaa tacgagggag gccggcagaa aaacggcttg cggtaaggca
      121 ggagaaatgt gtgccgcgaa tgaagtcctt gtatgactgg ataaagctcc agaggaaaac
      181 tttgtcaaac catgctgaaa tggtaaaggc gttcaactat atcctgaatc actggaatgc
      241 actgaatgag ttttgccggg tagtacttcc aatcattaga atatgtgctt ttcaatgaag
      301 gaaccgatga ctttttcgtg gatttccacg gaacgcgaga agcagattgt tttacgtgcc
      361 agccgcttga tgcgggggcg gagggtcagg ttgttacgtt catacgttgc gtgaagattt
      421 tgccggtcag gtgtttctcc tccggcactt ctctggcgta gctgccccag tcatcgctgg
      481 tcaccatgcc gatacagaac ggagtcaaca gggctaacag ttcccgacag gtctcatcat
      541 tccgtggccc gcaagtgtaa gccaacacac cccagtttta gtgttgtacg cataccaggg
      601 ccagtgttgt tgcgccttgc tgccaacgaa gcgccactgc tcatcaagtt cacagataag
      661 ggcaacatca gcgtgagcga ctggcgcaga tgttatccgc cggggcgaga gctttttaat
      721 gtccgtatga cggtattgat gccgattttc agtgttctgg cggtataaca gccccctgcg
      781 ccgttatgtg ccatttcaac gatctgctcc ttaacgccga gttttctggc ttcataagag
      841 taagtgagct ggaaaacacg tttacatgag cgacaacgga aacgctcatg ccgggaacaa
      901 ctacggccat gtcgataaac ttcatcggaa ttacagcgcg gacaatgaac agtaacggta
      961 gtcatggaaa acctcaaata ccagtagatt accagaaact tctatcgatt gaagacatta
     1021 ccttttgccg caataggtgg ggtaacattg gtgaaaacgc attacgccgg gtggcagttg
     1081 gaaaaaaatt atcttttttc ggctctgatc gtggtggaga aaatgcggca ataatctaaa
     1141 tagctgcgct taataccgct acacttttgc cagcccatgt ttgcctccgg ggaatgggct
     1201 gacggtttcc ataaaatggc gaactttttt caacagttgc cacattgagc ggcactgatg
     1261 attacgcgtt attgtgtcgt gaagtgcctg ccatagccgt tcaacatgat tcacccatgg
     1321 cgagtaaacc ggctgataaa tg
//
LOCUS       DAZPEB010000360         1332 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZPEB010000360 DAZPEB010000000
VERSION     DAZPEB010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.366"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(1..483)
                     /locus_tag="ACHURJ_004267"
     CDS             complement(1..483)
                     /locus_tag="ACHURJ_004267"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1282946.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRVVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCIQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            complement(474..776)
                     /locus_tag="ACHURJ_004268"
     CDS             complement(474..776)
                     /locus_tag="ACHURJ_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFR1282947.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
     gene            1048..1320
                     /locus_tag="ACHURJ_004269"
     CDS             1048..1320
                     /locus_tag="ACHURJ_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282948.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYKSVFADFITGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
BASE COUNT          392 a          334 c          283 g          323 t
ORIGIN      
        1 tcagacttta taaaacaagg tattaggtgc cacaactgac tgaacaaaac caaaacgctc
       61 ataaaatgct ctggcgtcat catcaattgc atgtaccata acagccttaa tgccaacctg
      121 atcagccaga ttgacaaccc ggtgaatggc atcacttagt agccatttac cgaaaccatg
      181 cccctggata caaacatcaa cagccagacg ccctaacaga acaacaggga caggattggg
      241 catgttatgc cgcaaactgc gaccaagctc ggcatgagtg acggaaccag tagctaatga
      301 gtagtatccc acaacacgta aagtatcctc caagcaaata acgaacgtgc gagaagcatt
      361 gagcatctgg tttttcatag cccgaccacg taaccagtta cttagcacct catttccaca
      421 gtcaaatggc tgaagtatat gctcatccgt caacaatgct ggcgcggtga catttatttc
      481 cattcaggct ttaccgccat cagacgttca cgcccttctg cattctgcac aggagcttcc
      541 agttgcgtaa taaacgaatc ataacgttcg ttatccagca caaataaacg ttgatccagg
      601 ataatgtttt tagcttcctg gtacgccagt tccatgatga aatcggtacg gttcttattg
      661 accaagctcg cagcataatc aataacggct cgttcctcat cagttgcccg gatgttaatc
      721 tgatgttctc gcggtgtttt acggacgctt gcagctgtag acataaaaac ctccattggt
      781 aacaatttgc tatgcaatta atgttatcgc aaatggtaac agaacgctat acagaatcac
      841 ttacgggaaa agcgatcccc tgataaaaac aatggcagag acctgaacat tgtgacgatg
      901 atctgtgtcg gcgcacaaat catgtctgat tacggccgtt taatgtgatt ttgataatga
      961 aatgcctgaa ccacggcagc ctggcggctg caaatcgggg tgtggcagaa tatgagcttt
     1021 cagagtaacc atccagaatg agttcccatg tcacacaacc tggagcacca gaaagtccac
     1081 accagaatgg tgaaagaggt tctgaaagcc gtcgccagag ccaataatca cccttataag
     1141 tccgtatttg cagactttat caccggtcat ccctcctgca cggtctgttt ctgggaaacc
     1201 ttccacaaaa tgtacccgga ttcaccgtac gaatacgtca cgttctgcca tacctgccgt
     1261 cgttttgatt tatacgaaac agaagcggag atgaaggcgg atgacccgaa gtggtggtga
     1321 ttttcagccc cg
//
LOCUS       DAZPEB010000361         1329 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZPEB010000361 DAZPEB010000000
VERSION     DAZPEB010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.367"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(170..499)
                     /locus_tag="ACHURJ_004270"
     CDS             complement(170..499)
                     /locus_tag="ACHURJ_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFR1282949.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(671..>1329)
                     /locus_tag="ACHURJ_004271"
     CDS             complement(671..>1329)
                     /locus_tag="ACHURJ_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282950.1"
                     /translation="TGEIDTALWRSGDVLLGSLLAMLFTGIWPQRAFIHWRIQLAKSL
                     TEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIAPASKETRIPKSIYEGIQT
                     INRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQQILLSLVHALYEGNPQPV
                     FANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNMETAHQLELLSNLICRALRK
                     "
BASE COUNT          326 a          311 c          312 g          380 t
ORIGIN      
        1 agtgcggtta ataacaatgg agttacagaa ctttttgcct tgctgcatac agaagatgtc
       61 tgtcgctaat ttcggtaagt tatcagatgg gaatgatgac ggtgatacac agaaaagaat
      121 caatgagcaa tgagggttgc caggcaaccc tcattgaata aaacaggaat tacttcgctt
      181 cgccgttttt catttcgccc atagctttca gctttttgga gatatcgcgg cgttctttgg
      241 agagctcggc atttttgatg atgtaatcgt caacgcgatc ttcatagtca cctttcatgc
      301 tggcgatgat gccctggatt gcttcaacgc tcatccccgg cttgatgtaa tcgctcaggt
      361 tgtccagcag caggacgcgc ttctggttgt cacggatctt tttctcaacg tcctgaattt
      421 cacgttgcag tttgttctta cgacggaaca gacgaacaaa ttccagtacg tcctggaatg
      481 aaggcttggt agtttccatt tttatacccc tgataatgtg agagtcggat tcgtttaagc
      541 aaccgctatc cgttagagcc aacattactc atagctgccg cagatgacaa tgcttttatc
      601 ccttttcact atcataccct ttatccttgc tgaatcgaag cagcagcaag atgattctgt
      661 agttcaggaa ttatttgcgc aaggcccggc aaatcagatt cgatagcaac tcaagctgat
      721 gtgccgtttc catgttcagc cacacataac catagattgg tgtttccaca accttcaggt
      781 catggtggtt attgagcaac tgacgcagct cttccactgc atcgttcaat ttttccgtat
      841 tggcaaaaac cggctgcgga ttaccttcgt acaacgcatg aacaaggctc agcagtattt
      901 gctgcatcat gtgctgggta tcacgaagtt tttgcgcgtt caataacacg aaatggctgg
      961 ggcgcgtggc ccagtacgca ttgatttgca actccagcat acaaaccaga ttgcggttaa
     1021 tggtctggat accttcatat atcgattttg gaatacgggt ttctttgctg gcgggcgcaa
     1081 tcagcccacg cattttcacg gcatcggtca ggagtttttg tagatggctt tccagacgtg
     1141 ggcgttcgag taagttcggt gagaatgcag attgatagac ccgattatac tcggtcagac
     1201 ttttcgccag ttgaatgcgc cagtggatga acgcccgttg tggccagata ccggtaaaca
     1261 acattgccag taaagagccg aggagcacat cgccgcttcg ccataacgcc gtatcaattt
     1321 cacctgtcg
//
LOCUS       DAZPEB010000362         1327 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZPEB010000362 DAZPEB010000000
VERSION     DAZPEB010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1327
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.368"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..78)
                     /locus_tag="ACHURJ_004272"
     CDS             complement(<1..78)
                     /locus_tag="ACHURJ_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HFR1282951.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSN"
     gene            267..797
                     /gene="cybB"
                     /locus_tag="ACHURJ_004273"
     CDS             267..797
                     /gene="cybB"
                     /locus_tag="ACHURJ_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFR1282952.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            1042..1206
                     /locus_tag="ACHURJ_004274"
     CDS             1042..1206
                     /locus_tag="ACHURJ_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282953.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKW"
     gene            1257..>1327
                     /locus_tag="ACHURJ_004275"
     CDS             1257..>1327
                     /locus_tag="ACHURJ_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR1282954.1"
                     /translation="MASISIRCPSCSATEGVVRNGKST"
BASE COUNT          351 a          285 c          304 g          387 t
ORIGIN      
        1 gttgcttttg acttcaagta atcgacgcaa caccagtcga ccgatacgac caaaaccgtt
       61 aataccaacc ttactcatgg ttttctcctg tcaggaacgt tcggatgaaa attgatcctt
      121 tccaggctta gcccaggatt gtgggatggg caatctccat tcttacagtg aaacgtaacg
      181 tactgaaaac gggtgaacaa tatttaatga aattttgaga aaagcccgtt atgttaacgg
      241 aaaattatgt taaagcagga aatgttatgg aaaataaata ctcaaggtta caaatcagca
      301 ttcactggct ggtctttttg ctggttatcg cagcgtattg cgcaatggag tttcggggtt
      361 tcttcccacg tagcgatcgg ccactcatca acatgattca tgtttcctgc ggcatctcaa
      421 ttctcgtgct gatggtcgtt cgtcttttgt taaggctgaa atacccaacc ccgccgatta
      481 tacctaagcc aaaaccgatg atgacgggac tggcgcattt gggacatttg gtgatttatc
      541 tcctgtttat tgcgctgcca gtgattggtt tggtgatgat gtataaccgg ggcaacccgt
      601 ggtttgcgtt tggtttgacg atgccttacg cttcagaggc caatttcgaa cgggttgata
      661 gcttaaagtc gtggcatgaa acgctggcga atctgggata ttttgtcatc gggttgcacg
      721 ctgcggctgc actggcacac cactatttct ggaaggacaa cacacttcta cgcatgatgc
      781 cgcgtaaacg ttcctgaagg atatttaaag aaaacgcctg tactaaaacc gacccgtggt
      841 acaggcgaag aatacgggtc tacatcggaa gcgcctatta tatttatttg tatgacaaat
      901 aaaaccataa tccttgccca tacgtccatc tggcttattt ttaatcaatt cacccgatct
      961 ttgatctcat caacggtatc aaaataaacc agcgtataac gttcaaatcg aacataagat
     1021 tcgataaacc atggaggtta tatgaaaaaa ctggcactta ttttgtttat gggaacgctt
     1081 gtttcctttt atgccgatgc cgggcgcaaa ccctgttctg gttcgaaagg ggggatctca
     1141 cactgtacgg caggcggcaa atttgtctgt aatgatggtt ctattagtgc ctcgaaaaaa
     1201 tggtaatgct gccaacttac tgatttagtg tatgatggtg attttaaggt gcttgcgtgg
     1261 cttctatttc catcagatgt ccttcctgct ccgctactga aggcgtggtg cgtaacggca
     1321 aaagcac
//
LOCUS       DAZPEB010000363         1314 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZPEB010000363 DAZPEB010000000
VERSION     DAZPEB010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.369"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..107)
                     /locus_tag="ACHURJ_004276"
     CDS             complement(<1..107)
                     /locus_tag="ACHURJ_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282955.1"
                     /translation="MNKTLIAAAVAGIVLLASNAQAQTVPEGYQLQQVL"
     gene            complement(400..>1314)
                     /locus_tag="ACHURJ_004277"
     CDS             complement(400..>1314)
                     /locus_tag="ACHURJ_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000535347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282956.1"
                     /translation="DIVRVSLPGRGSESITDFKGYYWYGFMEYIENITACDDVFSEYC
                     LDDENMSIQPEQINTPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRA
                     ILVLKEHEYSWALGGHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVNL
                     QSDNKIQGQGGFLSWSFLSFKKEFIDSCLNDKKCILHLPEFIFNDNKVRLVLDTDPEQ
                     RVNEVKNFLTALSDSICSLFIVNSNMASISLGNESFSTDDDLEYGYLINTGNHYDVYL
                     PPELFAQAYKLKNKEMNAQLDYLNRYAI"
BASE COUNT          411 a          277 c          218 g          408 t
ORIGIN      
        1 atgagcactt gctgtagctg atagccttcc ggtacggttt gtgcctgagc gtttgaagcg
       61 agtaaaacta tccctgccac agctgcggcg attagcgttt tgttcattcc tggcacctct
      121 tttgttatca atcactaaag agtgtgacag aaatatgact tttttccggg aacaggttcg
      181 caaaatggct tgttgaacgc ctgtttttct atatacatct tcaaaaacag gcaaggtttg
      241 acgcaaacta tgcctgatta gcccctgcct cgcgtacgcc gctgataatt gcacctgtgg
      301 ctaaacagaa tatctgcggc gttaccaata cagagacaat atttaccaat aaaaaagtgt
      361 gagactaata acaatggttt ttagcatatg cctttgccat taaattgcat aacgatttaa
      421 atagtcgagt tgcgcattca tttccttatt ttttaactta taagcctgag caaaaagttc
      481 aggagggagg taaacatcat aatgattgcc agtgtttatt aaataaccat actcaaggtc
      541 gtcatctgtt gaaaaggatt cattccccaa ggagattgat gccatattac tattaacaat
      601 aaataatgag caaatgctat ctgaaagagc agtgaggaaa tttttcacct cattaaccct
      661 ctgctctggg tcggtgtcta aaacaaggcg taccttgtta tcattaaata taaattcggg
      721 taaatgcagt atgcattttt tgtcattcaa gcaactatca ataaattctt ttttaaaaga
      781 caaaaaactc catgataaaa aaccaccctg cccctgaatt ttgttgtcag attgtagatt
      841 aacgccaaat aacgtcttta catcttccct gagattatag aatgccgtat caacaagagc
      901 ctcgatcgtt tgtttatcaa caattttaat aaactcttcg tatacttgct tttgcgtctt
      961 atggccgccc agcgcccatg aatactcatg ttcttttaaa accagtatgg ctcttaacag
     1021 acaatatcca tcgccatcta ctggagcgta ttttaatcca tatgttttat taatttcact
     1081 ctgaataaaa gatatgccag acaaatcaat accagtatct aaatcagata ttcccggcgt
     1141 atttatctgt tctggctgga tactcatatt ttcatcatct aaacaatact cagaaaaaac
     1201 atcatcacac gcagtaatgt tttcaatata ttccataaag ccataccaat aataaccttt
     1261 aaaatctgtg atagactctg aacctctgcc cggcaaggac actctaacaa tatc
//
LOCUS       DAZPEB010000364         1310 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZPEB010000364 DAZPEB010000000
VERSION     DAZPEB010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.370"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..214)
                     /locus_tag="ACHURJ_004278"
     CDS             complement(<1..214)
                     /locus_tag="ACHURJ_004278"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282957.1"
                     /translation="MRGQIVDKGRFVHAGYGMNALFGLQKQANSIYCRDDVDTGKRSA
                     SGNFAFIFTLKPRICGFIFNKIITLGE"
     gene            complement(239..>1310)
                     /locus_tag="ACHURJ_004279"
     CDS             complement(239..>1310)
                     /locus_tag="ACHURJ_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HFR1282958.1"
                     /translation="IEESSDDGDEYDYSPAKEEWVITAANAKPQCDIIHEAWQSRAVI
                     RYDMAVPLNLSERSARQSTGRVGVVLVVTLSHNSRRIDVDINLDNQADDHRLRVLVPT
                     PFNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYVALQEGRNGMAV
                     FSEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGLLS
                     CRLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMP
                     PVGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEEN
                     QGSNLSGPFLPGQSRTFSYRLA"
BASE COUNT          323 a          342 c          295 g          350 t
ORIGIN      
        1 actcacccag ggttattatt ttgttaaaaa taaagccaca tatacgtggc ttgagagtga
       61 agataaacgc aaaattacct gatgcgctac gcttgccagt gtctacatca tctctgcaat
      121 atattgaatt tgcctgcttt tgtagaccga ataaagcgtt catgccgtat ccggcatgaa
      181 caaagcgccc tttgtcaaca atctggccgc gcatatgtgg cttcggtttg attgctattc
      241 aggcaagccg gtaactgaac gtccgtgact ggccgggtaa aaaaggcccc gatagatttg
      301 aaccttgatt ttcctcggtg gtaatgtgtt catccatcat cgtttctgag caagaaatca
      361 cctcgcgact gaaagcaaca gtcgcatcac aggttgctga ttcagccgga ttaaacagcc
      421 gtaaaatcac ttcttgtcgg tcttcagctt tcttaagtgc gcttatcagg catcccactg
      481 ggggcatttt caacaaacta taactttccg gcacgttgaa tccggctttg ttgagcttca
      541 tcgcatccca tgggattttg ttgtaacact gtactggagt cagccatgct cgcgcctgct
      601 gagctacacc agcggcggtt ggcgtaccgg tataactcaa taaacttagg cgacaagaaa
      661 gcagaccacg tagttgtgag tccgggactg gcattttaat tcccgaaggc cgcccaggcc
      721 ttaaaagcag atcttctttg cccagtaagc ccacgccacg cagcaacgta atggcaaagg
      781 ttttcttctc ttcaccgatg acttcaaatt cacgtaaccc ttcgctaaag acagccatgc
      841 cgttacgccc ttcctgtaag gcaacatagt tgagcatatt ccataccgga accggcgctt
      901 ctttccagcc ttcttgctgc cagttgttca ttgcactgtc gttcacgggg cgcgttagcg
      961 aaccaaactg cgtatctgcc agaacactgt cggtgttaaa aggtgtaggg accaggacac
     1021 gaaggcgatg atcgtcagcc tggttatcaa ggttgatatc cacatcaata cgcctgctgt
     1081 tatgactaag agtgacaacc aacaccaccc ctactctgcc agtggattgc cgggcgctgc
     1141 gttctgacaa attgagcggc actgccatgt catagcggat aacagccctg ctctgccagg
     1201 cttcatgaat aatatcgcat tgcggtttcg cgttcgctgc ggtaattacc cactcttctt
     1261 ttgcgggtga atagtcatat tcatcaccat catcagagct ttcttcaatt
//
LOCUS       DAZPEB010000365         1303 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZPEB010000365 DAZPEB010000000
VERSION     DAZPEB010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.371"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..909
                     /gene="pocR"
                     /locus_tag="ACHURJ_004280"
     CDS             <1..909
                     /gene="pocR"
                     /locus_tag="ACHURJ_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein PocR"
                     /protein_id="HFR1282959.1"
                     /translation="SAATLNSELINKIAQDFAQATSLAVVVVNIHGDEISELFNFTPF
                     CQMMRQHPQLSTRCRMSDRCGGLEASKSDQPCIYRCHAGLTDFSIPLVIAGHLVGFVL
                     CGQVRLRNDYVDLVDILNVDDCWQADPELLNEFRKVPEMDYSRVMASADLLKLIVENC
                     LKKQLNFVVIKENKSQSDSVRQTRAPSPHDNKMKKALRYIDAHLSDDLRLEDVASHVY
                     LSPYYFSKLFKKYHGIGFNAWVNQQRMASARELLCHSDWSIASIARNLGFSQTSYFCK
                     VFRQTYQVTPQVYRQQVSENAAMETV"
     regulatory      998..1173
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
BASE COUNT          353 a          277 c          324 g          349 t
ORIGIN      
        1 tctgcggcga cacttaattc agaactcatt aataaaatcg cgcaggattt tgcgcaggcg
       61 accagccttg cggttgtcgt ggtcaatatt cacggcgatg aaatttccga gctgtttaac
      121 ttcactccat tttgtcagat gatgcggcaa caccctcagc taagcactcg atgtcgcatg
      181 agcgatcgtt gtgggggcct ggaagcctct aaatccgatc agccttgtat ttatcgctgt
      241 catgccggat taaccgattt ctctattcca ctggtaatcg ccgggcattt agtcggtttt
      301 gtattgtgcg ggcaagttcg tctgcgtaat gattatgtcg atttggtcga tattctgaat
      361 gtcgatgatt gctggcaggc agatcccgaa ttacttaatg aatttcgcaa agttcctgag
      421 atggattact caagagttat ggcctctgct gatttgttga agttgattgt agaaaattgc
      481 ctcaaaaaac aactcaattt tgtggttatt aaagagaaca aatctcagtc agattcggtg
      541 cgccagactc gtgcgcctag tccccacgac aataaaatga aaaaagcttt acgctatatt
      601 gatgcgcatt tatcagacga tttgcgtctg gaagatgtcg cctcgcacgt ttatctaagc
      661 ccttattatt tcagtaagtt attcaagaaa taccatggaa tcgggtttaa tgcgtgggta
      721 aaccaacaac ggatggccag cgcgcgtgag ttactgtgcc atagtgactg gagcatcgcc
      781 agcattgcgc gtaatttagg tttttcgcaa accagttatt tttgcaaagt tttccgtcag
      841 acttatcagg ttacgccgca ggtgtatcga cagcaagtga gtgaaaatgc ggcgatggaa
      901 acggtataga gtaaatgcta aaaaaaggga aggtttaaat aaacgctgga atgaataatt
      961 aacgttattt acgtgttatg ggaaaagtaa aatcagctca taacatacgc tgacaggtgt
     1021 ggcacatttg tgcctgggaa gagagtgaaa atccctcgca gcccccgctg ctgtaatgct
     1081 gacaaacccg aaaatgccac tgatcgcgag attgggaagg tcgggagagg atgacgctaa
     1141 gccagaagac cagccagtca gtcaatacca acaacttcgg tgggaagtgg gttgcccgga
     1201 agcgtccggt agagtacagg cgcgtcttta tacccctacc accctgaaag gatcagggta
     1261 tggcggcatc atttagttct ttcatcgcag tgccgacagg cac
//
LOCUS       DAZPEB010000366         1279 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZPEB010000366 DAZPEB010000000
VERSION     DAZPEB010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.372"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..835)
                     /gene="ipaH4.5"
                     /locus_tag="ACHURJ_004281"
     CDS             complement(<1..835)
                     /gene="ipaH4.5"
                     /locus_tag="ACHURJ_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HFR1282960.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQRLKYLKVGENQLRRL
                     SRLPQELLTLDVSNNLLTSLPENIITLPICTNVNISGNPLSTRVLQSLQRLTSSPDYH
                     GP"
BASE COUNT          360 a          230 c          287 g          402 t
ORIGIN      
        1 gcgggccgtg gtagtccggc gaagaggtta atctttgcag ggattgcaga acgcgagtcg
       61 acaatggatt ccctgaaatg ttaacattcg tacaaatggg caatgtgatt atattttcgg
      121 gtaatgaagt tagtaggtta ttggaaacat ccagtgtcaa tagttcttgc ggtaatctgg
      181 ataatctgcg tagttgattt tcacctacct tcaaatattt taagcgctga ggaaattctg
      241 gtagttcctg tagtctgttg ccggaaacaa agagttcttg tagtctatga ggcaattcag
      301 gcaagacagt aaggcgatta ccttcaatac gtaatattgt tagcgttgat ggaagctctg
      361 gtaacgtctc cagactatta aaacgtgccg agagtgattg taaatatggt ggtaatgatg
      421 gcagacatga caaatttcga ttgaatgaaa tattaagctt aatcagttga gatggaagtg
      481 tcgggagtat attcaagttg ttaccattta catgaagttc tgtcaaaagc ggagaatttt
      541 caggtaggga gattaactca ttgttggaaa tattaagttc acgtatatgc aaagggagtg
      601 gtggtaaaga acgtagtttc agtaagctta aattgagaac aggctcccgg gtatccagac
      661 atattttcaa taatcgaacc gcctggatgc gattttctgt tgttgttcct tccctagccc
      721 agtcatccca gatgcggtgg taatctctgg tacactgttc ttctaccgat aaacgagata
      781 tacatgataa gccacaaagg gaacgaaaaa aagaatgatt gttgatcggt ttcatttatt
      841 aacccctcac tattcatatc ttggtctatt aatgcaaata aaacgagaaa gtctttaaaa
      901 aaacaatcct agagctttat cggcgttttc attccagctg gaggaatatg ttcctgtctt
      961 tccagtaacc gtcgattgtt atatcagtct acccatgtta atgtgaccag ttctactttc
     1021 atccagtttg gattgatctt tatcccctat attttcaaac aatttttatc atttaactca
     1081 ttactgtctc gatattccca tagcaaacga tctggttggt tagcccctca aaaaagtcag
     1141 ttgcacgccg taaatccctg ccggtattga gcgaggattt acgggttgtt gttaaggtcc
     1201 gtaatcgggt cacagttttt ccggagtcaa tccggtctgc ggtttatgct tatgcgacgt
     1261 gattatgaat ggtgcagtc
//
LOCUS       DAZPEB010000367         1273 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZPEB010000367 DAZPEB010000000
VERSION     DAZPEB010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.373"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(57..896)
                     /locus_tag="ACHURJ_004282"
     CDS             complement(57..896)
                     /locus_tag="ACHURJ_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR1282961.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKTGAGISTPKVTRAHNRAEAVFLC
                     VMHSHIQIMVRRAGQPQGWPGSRVTGSANPVRLTTHEISTSAGELINLSLEDVIMATT
                     LSHPDVTIENGRAVTTSISIAEFFGKQHHHVVQKIESFECSELFLTSNFSRVKFEHRG
                     NSYNAYQITKNGFVFLVMGFTGKKAATFKEAYIAEFDRMEAELRQNNTPPADKMIPGD
                     GRTLVVHFDKFGNVEFTETVPDGALVCTLETFRFYLEKQGWTLVNRGAIKNMTVEQLL
                     SLK"
BASE COUNT          330 a          312 c          296 g          335 t
ORIGIN      
        1 tgagggcagg tgtaaaacaa taatcaagca tggggttcat atccatagct ccatttttat
       61 tttaaggaaa gcaactgctc tacggtcatg tttttaattg cgccccggtt tacaagagtc
      121 cagccctgtt tttccagata aaaccggaaa gtctccaggg tacagaccag tgcgccatca
      181 ggaacggttt cggtgaattc gacattgccg aatttgtcga agtgaacaac cagagtgcga
      241 ccatcacccg gaatcatctt gtcagcaggt ggggtgttat tctggcgcag ttcggcctcc
      301 atgcggtcga actcagcaat gtaggcttcc ttgaatgtgg cggctttttt gcccgtgaag
      361 cccatcacca ggaaaacgaa gccgtttttg gtgatttggt aggcgttata agaattgccg
      421 cggtgttcga atttaacccg cgaaaagttg ctggttaaaa atagttcaga acattcgaag
      481 gattctattt tttgaacaac atggtgatgc tgcttgccaa aaaactcagc aatcgaaata
      541 gaagtagtga cggcgcgacc attttcgatg gttacgtcag ggtgagaaag ggtagtagcc
      601 ataatgacat cctcaagtga taagttaatt aactcaccag cagaggtgct aatctcatgg
      661 gtggtgagac gcacagggtt agcactaccg gtcactcgag aacccggcca gccttgcggc
      721 tgccccgcac gcctcaccat aatttgaatg tggctgtgca ttacgcataa aaaaaccgct
      781 tcagcgcggt tatgcgctcg agtaactttc ggggtgctaa tcccggcacc cgttttatga
      841 ggtgcaggtg cactataatt ccacccgttc tggttttcaa tagctacatt caacattttc
      901 tctttccttt catcaccgaa gtgaattttg tgatgcggtg cctagtgcct ccaggtgacg
      961 ttaaccagtt aacaattaca gtcggctttc ccacccaaac caataaggac taacatgact
     1021 tttaactgtg ccgcgtgcgc ttagccgcat tcaccgcatc acaaaattca ctttaaaaag
     1081 ggcggacatc agccagcaat taaaccgatg ccgccaactg gtacttcaca cagcaatgtc
     1141 gttatttaca accggaagcg cactcccacc atttaaattt cacagacaag accgactctt
     1201 tatgaatacc ggaaatgcgc cttcgtgttg tgcccggttt tatttcacca cctccgggct
     1261 ttggtggcct cgg
//
LOCUS       DAZPEB010000368         1267 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZPEB010000368 DAZPEB010000000
VERSION     DAZPEB010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.374"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            7..747
                     /gene="phoN2"
                     /locus_tag="ACHURJ_004283"
     CDS             7..747
                     /gene="phoN2"
                     /locus_tag="ACHURJ_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HFR1282962.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAEDSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDENMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            820..942
                     /locus_tag="ACHURJ_004284"
     CDS             820..942
                     /locus_tag="ACHURJ_004284"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282963.1"
                     /translation="MGTPRFTPEFKGLLERLGHIPPAEAEKAYYAAIGNDDLAT"
     gene            complement(1100..1267)
                     /locus_tag="ACHURJ_004285"
                     /pseudo
     CDS             complement(1100..1267)
                     /locus_tag="ACHURJ_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
BASE COUNT          376 a          275 c          256 g          360 t
ORIGIN      
        1 taaattatga aaaccaaaaa ctttcttctt ttttgtattg ctacaaatat gatttttatc
       61 ccctcagcaa atgctctgaa ggcagaaggt tttctcactc aacaaacttc accagacagt
      121 ttgtcaatac ttccgccgcc tccggcagag gattcagtag tatttcaggc tgacaaagct
      181 cattatgaat tcggccgctc gctccgggat gctaatcgtg tacgtctcgc tagcgaagat
      241 gcatactacg agaattttgg tcttgcattt tcagatgctt atggcatgga tatttcaagg
      301 gaaaataccc caatcttata tcagttgtta acacaagtac tacaggatag ccatgattac
      361 gccgtgcgta acgccaaaga atattataaa agagttcgtc cattcgttat ttataaagac
      421 gcaacctgta cacctgataa agatgagaac atggctatca ctggctctta tccctctggt
      481 catgcatcct ttggttgggc agtagcactg atacttgcgg agattaatcc tcaacgtaaa
      541 gcggaaatac ttcgacgtgg atatgagttt ggagaaagtc gggtcatctg cggtgcgcat
      601 tggcaaagcg atgtagaggc tgggcgttta atgggagcat cggttgttgc agtacttcat
      661 aatacacctg aatttaccaa aagccttagc gaagccaaaa aagagtttga agaattaaat
      721 actcctacca atgaactgac cccataaagc tggacagcct gtatcaggct atggagggcc
      781 catagacaaa tctaccctat atgagcatag gaggagtcta tgggcacacc acgttttacc
      841 cctgaattta agggattact ggaaaggctg ggacatatcc ctccggcaga agcagaaaaa
      901 gcttattatg ctgccatcgg aaacgatgat ctggcaacct gagttcacag ataaaacatt
      961 ctctaggaaa ctcggggcgg ttccgttcac cacatgcaat gtggtgttgc aggggaacgg
     1021 tctgcaccat cccctatgtc gatcaatata acagaaatga caacttcaga ttcagggcac
     1081 aacctaaata tattttaggt cacctctcaa atcgtttgcc tgataccgct ccatttttta
     1141 ataaaaaaat caatcatttt tgaatctttg cttttcattg cattgtcaag catagtgtcc
     1201 ccactatttg ctttttgaaa ttttttgttt acatcaacca atccattatt gataaaatat
     1261 tctagca
//
LOCUS       DAZPEB010000369         1262 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZPEB010000369 DAZPEB010000000
VERSION     DAZPEB010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.375"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(1..1262)
                     /locus_tag="ACHURJ_004286"
                     /pseudo
     CDS             complement(1..1262)
                     /locus_tag="ACHURJ_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT          372 a          355 c          281 g          254 t
ORIGIN      
        1 gccaaccagt aaaacgcttc agttttcaac gcaccccaca gcatcggcca gaaaatgagt
       61 cctgcaattg cccctgtgcg cataatagcg cgggcaaatc cgaccccagt agggcgaata
      121 gcgggaagat aagagagtgt ggcaatggtc atgcccagcc caccaggacc cgctgaatgg
      181 aagaaaataa ttgatcccaa taaccctaaa gacagccagg gattagtcgt tgccagtgcg
      241 cctaacgaca gcaatgctaa ggtcacaaca gcaaatccat acatcgactg tcggcgagta
      301 cccagtcgtt ggagaataaa cgagccgatc atcccgcctg tcaccccaca aagggcattc
      361 acgacggcag aaccggtcaa cgcgccagta agcccgccac ttattattcc cgccaacgta
      421 agaggaagat aaacacctac ggcgttatat tgccacgcct gcatggttgc cacgacacag
      481 ccaagaatgg tgcgtttgcg gtaacgatca ttaaataaga tgccatatcc accactgtat
      541 ttattctctg ctttttccga gaatttcgct tctgttgtac ccgatgccgc aacgcgagcc
      601 tgaacaccat aacgttttcc cagaatgtcg cacgcttcct ggtaacgccc gacgcgtgcg
      661 gcccacattg cggattcacc aataaagaag taacgcaaaa tcatgaagat gactgcaaaa
      721 atagctccga tgaatattcc gtaacgccac aattgttctt cagcgatacc cgacagaagc
      781 agcggcaaaa gtaccaaata aacaacggtt gtcgaaacat accaggccat ttgccagagg
      841 ttgaccgacg ttccggtttt cttcgacgtt gaaccacgaa gctctgccac ggcattggtt
      901 gccaaaggga agtcgatgcc taaaccaaag cccatgatga cgcggcacac cagcacccac
      961 cagatactgg gagcaatagc taccgctgca gcaccgatgg tgcacagccc catgccaata
     1021 ataaatgccc gcttttgccc aaagcggttt attactcggt tcgctaataa accgccaatt
     1081 aacgcggcga ttaatactga tgcattgacg gttgcagcaa gacccggcga aatgccgaat
     1141 tgagcggcaa tgtatttatt accaaacccc accatcgccg cctagtaggc atcaatcaat
     1201 atgccgccca aagcaataaa aataacgccg atactggtgt tgattgcaga gttcttatta
     1261 ac
//
LOCUS       DAZPEB010000370         1235 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZPEB010000370 DAZPEB010000000
VERSION     DAZPEB010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.376"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            36..752
                     /locus_tag="ACHURJ_004287"
                     /pseudo
     CDS             36..752
                     /locus_tag="ACHURJ_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            782..>1235
                     /locus_tag="ACHURJ_004288"
     CDS             782..>1235
                     /locus_tag="ACHURJ_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CS1 type fimbrial major subunit"
                     /protein_id="HFR1282964.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLET"
BASE COUNT          393 a          239 c          262 g          341 t
ORIGIN      
        1 actttttgga aacaatattg taagatcaaa tttatatggt ggcgaaaatt aatctcttat
       61 cacggcttgc gccgctttta ttcgtgttcg ctcctttttt ggcacagtca aatatgactg
      121 tttatcctat ggctgtttcc attaatagtc aggatgaagg caacgttcgc gtaatatcta
      181 aaagtaatga agttcagtac atcaaggcga cagtattccg tatcgataat ccatcgacgc
      241 ctcaggaaaa tgaagttgag attaaatccg gtgatgcaaa tcatctggtt gttatgccac
      301 ctaaattcgc tttaccagcc ggtagtagca aaactgtgcg ctttgttgca atggaaccag
      361 agcaaaaaga aaaaaattaa cgcgttaaat ttgaagcggt tcccagtatt gatgacgttg
      421 ccacagataa aaaagatttc tctatgcagt taactgttag cttaatttgg gggattgttg
      481 ttagtgttcc acctcagcaa cctattgcta agttagaagt aaatgctgcc caaaaattag
      541 ttaatgcagg gaatcaacgc ttaaagattt taacgatcgc ttattgtaaa aataatagca
      601 aagaaaattg taagatacag accgtaaata aaaacatctt ccctggtcag gaaagaaatc
      661 ttgaaagcat atctggctac gacaaaatcg ttgtcaaata taataactgg atcaccaaag
      721 ataatggcga gtttgaactg gcggtccatt aattatcatt gtaataaacg aaggaatagt
      781 aatgaaaaag gtatttgcaa aatctcttct ggtcgcagcg atgttttctg ttgcaggctc
      841 cgcattggca gtacaaaagg atattactgt aacggccaac gttgatgccg ctctggatat
      901 gacacagacc gataacaccg cgttgccgaa agcagtagaa atgcaatatc tgccggggca
      961 gggtctgcaa tcttaccagt tgatgaccaa aatctggtct aacgacgtta ccaaagatgt
     1021 aaaaatgcag ttagtttccc cggcgcaact ggttcaaagc ctggatgcta gcaagattgt
     1081 tccgctgaca gtaacctggg gcggtgaaga aattaaagct gatgcggcaa ccacttttac
     1141 cgcaaccaaa atctttgctt ccgatgcgtt aactaacggt tctctggcta aaaacctgat
     1201 gtttgctcaa accaccaaag gtgttctgga gacag
//
LOCUS       DAZPEB010000371         1235 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZPEB010000371 DAZPEB010000000
VERSION     DAZPEB010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.377"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..1214
                     /locus_tag="ACHURJ_004289"
     CDS             join(<1..292,292..1214)
                     /locus_tag="ACHURJ_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR1282965.1"
                     /translation="MTKNTRFSPEVRQRAIRMVLESQGEYDSQWAAICSIAPKIGCTP
                     ETLRVWVRQHERDTGGGDGGLTTAERQRLKELERENRELRRSNDILRQASAYFGEGGV
                     RPPLEKVMPLLDKLRKLYGVGPVCSELHIAPSTYYHCQQQRHHPDKRSARAQRDDWLK
                     KEILRVYDGNHQVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVLRGKKVRTTIS
                     RKAVAAGDRVNRQFVAERPDQLWVADFTYVSTWQGFVYVAFIIDVFAGYIVGWRVSSS
                     METTFVLDALEQALWARRPSGTVHHSDKGSQYVSLAYTQRLKEAGLLASTGSTGDSYD
                     NAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNRRLLERLGHIPPAEAEKA
                     YYASIGNDDLAA"
     misc_feature    282..398
                     /locus_tag="ACHURJ_004289"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
BASE COUNT          301 a          298 c          366 g          270 t
ORIGIN      
        1 atgactaaaa atactcgttt ttcccccgaa gtccgtcaga gggcgattcg tatggttctg
       61 gaaagtcagg gcgaatatga ctcacagtgg gcggcaattt gttccattgc cccaaagatt
      121 ggctgtacac cggagactct gcgtgtctgg gtacgccagc atgagcggga taccggaggc
      181 ggtgatggcg ggctcaccac cgctgaacgt cagcgtctga aagagctgga acgtgaaaat
      241 cgtgaactgc gccgcagtaa cgatatcctt cgccaggctt ccgcttattt tgcgaaggcg
      301 gagttcgacc gcctctggaa aaagtgatgc cactgctgga taagctgcgt aagctgtacg
      361 gggtcggacc ggtatgcagt gaactacata ttgccccgtc aacgtattac cactgtcagc
      421 aacagcgaca tcatcctgat aaacgcagtg cccgtgcgca gcgcgatgac tggctgaaga
      481 aagagatact gcgcgtatac gatgggaatc atcaggtata cggtgtgcgt aaagtctggc
      541 gtcagttgtt acgggaaggt atcagagtgg ccagatgcac tgtggcacgt ctcatggcgg
      601 ttatgggact tgccggtgtt ctccggggta aaaaggtccg tacgaccatc agccggaaag
      661 ccgttgccgc aggcgaccgc gtaaaccgtc agttcgtggc agaacgacct gaccagctgt
      721 gggtggctga ttttacttac gtcagcacat ggcagggctt cgtctatgtg gcgttcatta
      781 ttgatgtgtt tgccggatac atcgtggggt ggcgggtctc atcgtccatg gaaacgacat
      841 tcgtgctgga tgctctggag caggcgttat gggcccgtcg accgtccggc acggtccatc
      901 acagtgataa aggttctcag tatgtatcgc tggcctacac acagcggctt aaggaagccg
      961 gattactggc atcaacagga agtacaggcg actcgtatga caacgcgatg gcggagagca
     1021 tcaatggtct ttacaaagcg gaggtaatac accgtaagag ctggaaaaac cgtgcagaag
     1081 tggaactggc cacactcacg tgggtggact ggtataataa tcgacgattg ctggaaaggc
     1141 tgggccacat ccctccggca gaagcagaaa aagcttatta tgcttccatc ggaaacgatg
     1201 atctggcagc ctgagttcac agataaaaca ctctc
//
LOCUS       DAZPEB010000372         1233 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZPEB010000372 DAZPEB010000000
VERSION     DAZPEB010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.378"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..184)
                     /locus_tag="ACHURJ_004290"
     CDS             complement(<1..184)
                     /locus_tag="ACHURJ_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282966.1"
                     /translation="MKNIKLLGSAMLVSLALFSQNSLAKEYKIGASLLTQQHPFYIDL
                     ANAMKEEAKKDNVNLSV"
     gene            complement(220..1074)
                     /locus_tag="ACHURJ_004291"
     CDS             complement(220..1074)
                     /locus_tag="ACHURJ_004291"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose bisphosphate family class II aldolase"
                     /protein_id="HFR1282967.1"
                     /translation="MYIISSKNMLKKAQSEGYAVPAFNIHNLETLQVVVESAAQLRSP
                     VMLAGTPGTYRYGGVGSLISLVQSLAREYNLPLVLHLDHHEESDDIFNKVRAGIRSVM
                     IDGSHLPFEDNIARVQEVTTFCHRFDVSVEAELGRLGGQEDDLLVDEKDSAYTSPAAA
                     AEFVQRTQIDSLAVAIGTAHGLYAHEPRLDFVRLEQIRQRVEIPLVLHGASGLSEQDV
                     QHCIRRGICKVNVATELKIAFADALKGYFYNNPAANDPRHYMQPAKAAMKEVVTRIIG
                     VCGSEGKI"
BASE COUNT          352 a          289 c          253 g          339 t
ORIGIN      
        1 agacacttaa attgacattg tctttttttg cctcttcttt catggcattc gcaagatcaa
       61 tatagaaagg atgttgctgt gttaataatg acgcaccaat tttatactct tttgccaacg
      121 aattttgcga gaacaatgcc aggctcacca acatagctga accaagtaat ttaatatttt
      181 tcataaacaa tcctcattgt ttttgtaggg tacaacgtct taaatttttc cttcactacc
      241 acagacacca ataatacgag ttacgacttc tttcatggcg gcctttgcgg gctgcatata
      301 gtggcgtgga tcatttgccg caggattgtt gtagaaatat ccttttaacg catcggcaaa
      361 cgcaattttc aattctgtcg cgacattcac tttacaaata ccgcggcgaa tacagtgctg
      421 aacgtcctgt tctgataagc ccgaagcacc atgcaacacc agtggaatct ccactcgctg
      481 acgaatctgt tccagccgca caaaatccag tcgtggttca tgcgcatata aaccatgtgc
      541 tgtgccgatt gctacggcca gggagtcaat ttgggtacgt tgaacaaact ctgcagctgc
      601 ggctggtgag gtataagcgg aatctttttc atccaccagc agatcgtcct cctggccgcc
      661 aagacgccct agctcggcct caacgctaac atcaaagcga tggcaaaagg tggtaacttc
      721 ttgcactcgc gcaatgttgt cttcaaatgg caaatgggag ccatcaatca tgacagaacg
      781 gatccctgcg cgaaccttgt taaaaatgtc atcactctct tcatgatggt cgagatgcag
      841 gactaacggc aggttgtact cgcgagccag cgattgtact aacgaaatta aacttcccac
      901 gccaccataa cgataagtac cgggtgttcc cgccagcata acgggagagc gaagttgtgc
      961 agcgctctca acaactacct gtaaagtttc aaggttatga atgttgaaag cggggacggc
     1021 atatccctct gactgggctt ttttcagcat gtttttgctt gaaatgatgt acatcgcagc
     1081 tcctgttatt tgtcgttcgc cttacaaaat gaagaaagag aatattcact ttcattttga
     1141 aagcaaacta gcacagcaaa agcggcaaaa agcgtgatac atgtcgaata tcatcaaaat
     1201 aacttacaaa aaaataactt aatttgtttt tca
//
LOCUS       DAZPEB010000373         1226 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZPEB010000373 DAZPEB010000000
VERSION     DAZPEB010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.379"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(403..1122)
                     /locus_tag="ACHURJ_004292"
     CDS             complement(403..1122)
                     /locus_tag="ACHURJ_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HFR1282968.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQFFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
BASE COUNT          382 a          245 c          227 g          372 t
ORIGIN      
        1 tattagattg cagttactaa tagtaaaagc ctccttacaa ggaggtagct tattcagaat
       61 gctcctataa gatagcgagc tcgtcaacga tacagatgat ctgattatat tgtgcaaaag
      121 gagaacagga aaataggcac tggaaaagat gaaggcctga tgggagcatt aactaatgca
      181 aaatccgaca ggttaatatc ccgaaaagca atcaaacgac tcaaacagga gatgcacgct
      241 gaagtggtgg acaactgttc ggatttcaga tgagtagctc agcagtttat gagccgaatg
      301 ctctgtcacg gtcaggaatc aggctgcata cgctgtgcca aatctaatcg tagttattct
      361 aaccagtttt ccagctacaa ggtggtgtag ctggcatctt ctctactcta tcatcaaacg
      421 ataaaatggt tcctctcgta acatttgctc ttgcctttca cttccatctc gatcgcttcg
      481 tagttcatta cgatagctga catatttcca gtcttcaggt ctaacatctg acgccggata
      541 ctccccggga gcgattttgc ttcttaatag attagactcg agacacatta taaattgtcg
      601 tattttatga agtaataaag cactatattg cgagcattcc tgatctgatt ttacatatag
      661 cgtaaactga gctcctatcc ccacacgaga ttgctgagaa actcgattca tatcagttat
      721 cttccattta tctataggac tgtcctctga aaataataaa ccagacaaaa tttggaatgc
      781 cagaggaact tgctccctag ctatactaat atgaaacttg tccccaacaa aatccccctt
      841 actctgagga ttttcacgac atgcatgaat aaacacatct ccactatggt tcacaatctg
      901 gaatccttgg tacatgacag aaaaaaattg acatatatca gagtaagttg gcacgttcga
      961 tgctactttg aaatagtacg ctgggtattg ccgtatttgg ttgtacaata tattgaagtt
     1021 atttttcaat ctttcattcg cagaaagcat ctgcggtatt tcagacctca caacattcaa
     1081 agaatctaaa tttagtttta gacagggctt ttttatgggc atagaaaacg tcctctataa
     1141 aatagatata ttatcttata ttgattgttt atcatctttt ataaaacgat acattatatg
     1201 taatgtatgt aagcgtaagg gtcttc
//
LOCUS       DAZPEB010000374         1211 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZPEB010000374 DAZPEB010000000
VERSION     DAZPEB010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.380"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            1076..>1211
                     /locus_tag="ACHURJ_004293"
     CDS             1076..>1211
                     /locus_tag="ACHURJ_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282969.1"
                     /translation="MFKYAEMLMAEEDNKVCELIFKQQDNLILKINDRFPRIIIKKYR
                     T"
BASE COUNT          370 a          209 c          237 g          395 t
ORIGIN      
        1 cttcaaaata aacgtttaat agggacaatc accatttgga ttgtccgctg aggaggcgaa
       61 cgttttccta gacagcttta tcatgtcagc ccacattgtt ggcgctgaca atactaagga
      121 caaacgatgg ggaagtagct ttttagcgtt attttcggga gtaaaaaaca tggtatcttt
      181 ccgtatgtca gttgtatctt gctggtattc gtagtcgtga taaggcatac aacgatattt
      241 atcttcacgt tagtgaagaa taagttgttt tgtgatctat gttaatctgg gattacaatc
      301 cgggcttgat acatcgcatc acgactttta ttctcttcag aatagaatat gttactcccc
      361 gttttacagg ctcaggattt gaattacaaa ttatactttt aagtgaacaa taactagggg
      421 tatttcttat ttcctgaaac caggcttggc ccatttcggg atgagtttga aaaaactctt
      481 gacgcatacg atagtgatga aataaacgaa acaaattacg tcaacaaact caggcgtctt
      541 gcctgacatg ctcctgacat cattgatatt cacgctcatc tggcatatgc atttgcgctc
      601 tttatgctgc aattctggcg aatgttcatt tacagcgtca tcaggatgcc gttatactca
      661 ttgataaaat actaacatat aaccctgaga ataatcaggg gacacgctgg ttactgggga
      721 cggaattgtt acgtgctggc aagcatgaca gggctctgta cgtattgaca ggacatgtca
      781 atgaatttcc cccctactgg tacgagcttg ctcttttaca ttttatgaat gaggcatata
      841 ttaatgctgc aacggcattt cgatatggat ttttaacaag catatattgt tgaaatgctt
      901 tgttagaatc tttatccatt ccctggggct gtatagtatg attttttagg tatttctgaa
      961 tacagccaaa tattattgca aaatattctc taaattatta gttaaatatc cataagcatt
     1021 attgcctggg aactggacat ataattactc ttctgtgcta aaggaacgtt ctgaaatgtt
     1081 taagtatgca gagatgttga tggcggaaga agataataaa gtatgtgaac ttatttttaa
     1141 acaacaagat aatttgattt taaaaataaa tgatagattt cccagaataa ttataaagaa
     1201 ataccgaaca t
//
LOCUS       DAZPEB010000375         1206 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZPEB010000375 DAZPEB010000000
VERSION     DAZPEB010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.381"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(48..>1206)
                     /locus_tag="ACHURJ_004294"
     CDS             complement(48..>1206)
                     /locus_tag="ACHURJ_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HFR1282970.1"
                     /translation="RFGNEMSGWFDLTLNQRVYNQDGKTANAVVTYDGNVGEQYNDAW
                     FGDSANENIMQFSDIYLTTRGFLPFAPEADFWVGKHKLPQYEIQMLDWKTLTTDVAAG
                     VGIENWALGVGLFDMSLSRDDVDVYSRDFSRTSQMNTNSVDVRYRNIPLWDDATLSLM
                     AKYSAPNKTDQQQDNENDNSYFEMKDSWMLTSVLRQNLQRDTFNEFTLQVANNSYASS
                     FASFSDASNTMAHGRYYYGDHTNGIAWRLISQGEMYLTDNIIMANALVYSHGEDVYSY
                     ESGAHSDFDSIRTVIRPAWIWNTWNQTGLELGWFKQQNKTQQGVTLNESAYKTTLWHA
                     LKVGESILGSRPEIRFYGTYINILDNELSNFKFNENSKDEFMAGIQAEVWW"
BASE COUNT          345 a          293 c          221 g          347 t
ORIGIN      
        1 aaaccttttt taaatcctga catacacaac tccttaacta aaatagatta ccaccagact
       61 tccgcctgga tgccggccat aaactcgtct ttgctgttct cattaaactt aaaattcgat
      121 aattcgttat ccagaatatt gatatacgtt ccatagaagc gaatttctgg tcgtgaacct
      181 aaaatgcttt cacccacttt caatgcatgc cagagtgtcg ttttataagc cgattcattt
      241 agtgttacac cctgctgagt tttgttctgt tgcttaaacc agcctaattc aagccccgtc
      301 tgattccatg tattccagat ccaggccggt cttattacag tgcgaatact gtcaaaatca
      361 ctatgagcac cactttcata actataaaca tcttcgccgt gagaatagac aagcgcgtta
      421 gccataataa tattgtcagt aagatacatc tcgccctgag agattaaacg ccaggcgatc
      481 ccattggtat gatcaccata gtaatagcga ccatgcgcca tcgtattact ggcatctgag
      541 aaactggcaa aactgctggc ataggaatta ttggcaacct gtaacgtaaa ttcattaaac
      601 gtatcgcgtt gcaggttttg ccgtaaaaca gaagtcagca tccagctatc tttcatttca
      661 aaataactgt tgtcattttc gttatcttgt tgttgatccg ttttattagg tgcggaatat
      721 ttagccatta atgataatgt cgcatcatcc cataacggga tattgcgata acgaacatcc
      781 acagaattgg tattcatctg gctggtacgc gaaaaatcac gagagtaaac atcgacatca
      841 tcacggctta aggacatatc aaacagccct acaccaagtg cccagttttc aatccccaca
      901 cccgcggcga catccgtggt taaggttttc cagtccagca tctggatctc atattgcggg
      961 agtttatgtt tgccgaccca gaagtctgcc tctggtgcga agggtaaaaa acctcgcgtt
     1021 gtcaggtaaa tatcgctgaa ctgcatgata ttttcattgg cactgtcacc aaaccaggca
     1081 tcgttatact gctcacccac gttgccatca taggtaacga ccgcatttgc cgttttaccg
     1141 tcctgattat aaacacgctg atttaaggtc aggtcaaacc aaccgctcat ctcattacca
     1201 aaacgt
//
LOCUS       DAZPEB010000376         1198 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZPEB010000376 DAZPEB010000000
VERSION     DAZPEB010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.382"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            59..1069
                     /locus_tag="ACHURJ_004295"
     CDS             59..1069
                     /locus_tag="ACHURJ_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFR1282971.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQELKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVASFRAPGFARSQHIVPARNCHVAPASLPLKVLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDAVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
BASE COUNT          283 a          293 c          324 g          298 t
ORIGIN      
        1 tcgatgaagg tatcgcgcaa cgctaatcct ctatggatat cgattctgga gaataacaat
       61 ggataaaatt acccaggttt tattttcaga tattgggaaa gtcaccactc aatatgttga
      121 agtaccacac caggaactta aaccgcacga agtgcggatt gcgcctgtgt tctatgggat
      181 atgcggttcg gatctgcatg ttctgaaagg cggtcatccg tttgccaaac cacctgtcgt
      241 ccccggtcat gaaattgcag cgcgcgttac ggaagttggc agcgacgtta aaaatgtaca
      301 gccgggcgat catgttgtgg tcgatcccat catggcttgc atggaatgcc gagcctgcaa
      361 agcaggacgt tttaatcttt gtgaaccacc gcaggttgct agttttcgcg caccgggctt
      421 tgctcgctca caacacattg ttcctgcgcg taattgccat gtcgcaccag cctctttacc
      481 gctaaaagtg ttggcctttg ccgaaccggc ggcttgtgcc cgtcactgcg ttaaccgaat
      541 gccgaaagct tctctggaaa gcgtactggt aattggtgcc ggaacgatag gcttatccat
      601 cgtgcaggca ctgcgcatta tgggggcagg taagattacc gtgattgaac ctgacgctgc
      661 caaacgcgcg ctggcgttaa agctgggcgc agcagaagtt tgggcaccag gtgagctggc
      721 cgcagatgtg cgatttacgg gggcgattga tgtcgttgca gcgcaggcca cgcttaacga
      781 tgcatgtacc cgtgtatatg ccggaggcac cgtcgtgtgc atgggcgtac caagtgggcc
      841 gcgtgaaata ccattaccga tgatgcaacg tttcgagcgt gacttgctca actctggcat
      901 gtacatccct gaagatttcg atgctgttat cgaatggctg gcggatgggc ggtttgatac
      961 cagtgaactg gttaccgatt tatttgccat tgaggatgca gcggcggcat ttgaacgcgc
     1021 gcagcaaaat gactccataa aggtcatgct gcaatttgcg ccggaatgat tttctaaatc
     1081 tctgtctggc gctgcgtcac gtcagcgcca ccctacaaca attaaaaagg acactgcgat
     1141 gagtcaaatc accagtcctg caacttattc aattagtcgg ccacaggacg taattgat
//
LOCUS       DAZPEB010000377         1182 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZPEB010000377 DAZPEB010000000
VERSION     DAZPEB010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.383"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..1077
                     /locus_tag="ACHURJ_004296"
     CDS             <1..1077
                     /locus_tag="ACHURJ_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HFR1282972.1"
                     /translation="NIFVKEYNGKGICCASTRESKHIANMWLSKVVDDEGKEIFSGIR
                     HGVISAYGLKKNSSERAVAARNKAEELASAALYSRQELLSQALSGKTVDLKIVSTSLL
                     TPTSLTGGEESMLKDQVKALKELNSKRGEPTKLLIRNSDGLLQEVKVNLKVVTFNFGV
                     NELALKMGLGWRNVDKLNGESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKNDVIYLA
                     NQIKEILNKKLQKNDNGEPYKLSQRMALLAYTIGAVPCWNCKSGKDRTGMQDAEIKRE
                     IIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKKLPLSSL
                     ELSYAERIGDPKIWNMVKGYSSFV"
     gene            1087..>1182
                     /locus_tag="ACHURJ_004297"
     CDS             1087..>1182
                     /locus_tag="ACHURJ_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR1282973.1"
                     /translation="MEDLADVICRALGIPSIDTDDQAIMLDDDVLI"
BASE COUNT          419 a          145 c          286 g          332 t
ORIGIN      
        1 aatatatttg tcaaagagta taatggaaaa ggaatttgtt gtgcttctac gagagagagt
       61 aagcatattg cgaatatgtg gctttcaaaa gtggtggatg acgaggggaa ggaaattttt
      121 tcgggtatca gacatggagt tatatcagcc tatggattga aaaaaaactc atctgaaaga
      181 gctgttgcag ctcgtaataa agctgaggag ttagcaagcg cagcattata tagcagacaa
      241 gaattattat cacaggcttt gtctggtaaa acagtagatt taaagattgt ttcgacttct
      301 cttctgacgc cgaccagttt aactggggga gaggagagta tgttaaaaga tcaggtaaag
      361 gcattaaaag agttaaactc taaaaggggg gaaccaacga aattattaat tcggaatagt
      421 gatggtcttc tgcaagaagt aaaggttaat ctaaaagtgg tgacatttaa ctttggtgta
      481 aatgaattag cgctaaaaat gggcttaggc tggaggaatg ttgacaagct taatggtgaa
      541 tcaatatgtt ctttgttggg ggataatttc ctcaaaaatg gtgtgattgg aggttgggct
      601 gcagaagcta tagaaaaaaa tccaccatgt aagaatgatg tgatatatct ggctaaccag
      661 ataaaagaga ttctaaacaa gaagttacaa aagaatgata atggagagcc gtataaattg
      721 tcacaaagaa tggctctatt ggcttatact ataggtgctg taccttgctg gaattgtaag
      781 agtgggaagg acagaacagg tatgcaggat gctgaaatta aaagagaaat aataagaaaa
      841 catgaaacag ggcagttttc tcaattaaat agtaaattat cctcagaaga gaaaagatta
      901 ttttctacta ttctaatgaa tagtggtaat atggaaatcc aagagatgaa tactggtgtg
      961 cctggaaata aagttatgaa aaaattaccg ctatcttcgt tagagctgtc ttatgctgaa
     1021 agaatagggg accccaaaat atggaatatg gtgaaagggt attcgtcatt tgtataagag
     1081 gaatatatgg aagatttagc agatgttatt tgccgagcct tgggtatccc ttcaattgat
     1141 accgatgatc aagcaatcat gcttgatgat gatgtgttaa ta
//
LOCUS       DAZPEB010000378         1168 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZPEB010000378 DAZPEB010000000
VERSION     DAZPEB010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.384"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..1096
                     /gene="ospD3"
                     /locus_tag="ACHURJ_004298"
     CDS             <1..1096
                     /gene="ospD3"
                     /locus_tag="ACHURJ_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HFR1282974.1"
                     /translation="EKHQKCYGLISDGMSIFVDRHTPTSMSSIIRWPNNLLHPKVIYH
                     AMRMGLTELIQKVTRVVQLSDLSDNTLELLLAAKNDDGLSGLLLALQNGHSDTILAYG
                     ELLETSGLNLDKTVELLTAEGMGGRISGLSQALQNGHAETIKTYGRLLKKRAINIEYN
                     KLKNLLTAYYYDEVHRQIPGLMFALQNGHADAIRAYGELILSPPLLNSEDIVNLLASR
                     RYDNVPGLLLALNNGQADAILAYGDILNEAKLNLDKKAELLEAKDSNGLSGLFVALHN
                     GCVETIIAYGKILHTADLTPHQASKLLAAEGPNGVSGLIIAFQNRNFEAIKTYMGIIK
                     NENITPEEIAEHLDKKNGSDFLEIMKNIKS"
BASE COUNT          401 a          210 c          236 g          321 t
ORIGIN      
        1 tgaaaaacat cagaaatgtt atggtcttat atccgacggt atgtctatat ttgtggacag
       61 acatactcca acaagcatgt cctccataat cagatggcct aataatttac ttcaccccaa
      121 agttatttat cacgcgatgc gtatgggatt gactgagcta atccaaaaag taacaagagt
      181 cgtacaacta tctgaccttt cagacaatac gttagaatta cttttggcag ccaaaaatga
      241 cgatggtttg tcaggattgc ttttagcttt acaaaatggg cattcagata caatcttagc
      301 atacggagaa ctcctggaaa cttctggact taaccttgat aaaacggtag aactactaac
      361 tgcggaagga atgggaggac gaatatcggg tttatcccaa gcacttcaaa atgggcatgc
      421 agaaactatc aaaacatacg gaaggcttct caagaagaga gcaataaata tcgaatacaa
      481 taagctgaaa aatttgctga ccgcttatta ttatgatgaa gtacacagac agatacccgg
      541 actaatgttt gctcttcaaa atggacatgc agatgctata cgcgcatacg gtgagctcat
      601 tcttagcccc cctctcctca actcagagga tattgtaaat ttgctggcct caaggagata
      661 tgacaatgtt cccggacttc tgttagcatt gaataatgga caggctgatg caatcttagc
      721 ttatggtgat atcttgaatg aggcaaaact taacttggat aaaaaagcag agctgttaga
      781 agcgaaagat tctaatggtt tatctggatt gtttgtagcc ttgcataatg gatgtgtaga
      841 aacaattatt gcttatggga aaatacttca cactgcagac cttactccac atcaggcatc
      901 aaaattactg gcggcagaag gcccaaatgg ggtatctgga ttaattatag cttttcaaaa
      961 taggaatttt gaggcaataa aaacttatat gggaataata aaaaatgaaa atattacacc
     1021 tgaagaaata gcagaacact tggacaaaaa aaatggaagt gattttctag aaattatgaa
     1081 gaatataaaa agctgaatat tatctcgtgg tgatacactg aatttgaggt aaccggctca
     1141 tttaaaccgt ctggtctgtt acctccgg
//
LOCUS       DAZPEB010000379         1165 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZPEB010000379 DAZPEB010000000
VERSION     DAZPEB010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.385"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(92..1075)
                     /locus_tag="ACHURJ_004299"
     CDS             complement(92..1075)
                     /locus_tag="ACHURJ_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFR1282975.1"
                     /translation="MSNDYPLVSIIIPTYNSSDYITETLTKLEKQTYPNFEIVIVNDG
                     SKDNTSNVLREYGLTHSRLIIVNKENGGVSSARNTGIRKAQGQFICFMDDDDEIDPNY
                     LLKMYTRQHETGGDAIYCGLYGHYIKDGVTYSPINTEFNEGSLLFDFFYKKVRFHIGC
                     LFIRKQLLEENNLFFDEDLRLGEDLDFIYRLLITCDMYAVPYYMYKHNYRENSLMNSC
                     RTITHYRHESFAHERIYSSVMQLYKGNRKEEIHTLLSQNRAYHKTRYLWNVLLNGDFK
                     LLNQLVESNEKELRYCNLPGKRDKRRAKILASKNYILWRMVRLVNRKKNKR"
BASE COUNT          372 a          215 c          157 g          421 t
ORIGIN      
        1 ttaaagactt tataccagaa ttaatttcat tccaacaaaa cgacatataa tttcaaccta
       61 tgctacgagg atgggttatt taatgcaaga tctaacgttt attctttttt ctatttacca
      121 gtcttaccat cctccagaga atataatttt ttgatgctaa tatttttgct cgtctcttat
      181 ctctcttgcc aggaagatta caatatctta attctttttc attgctttca actaattgat
      241 tcaatagttt aaaatcacca ttaagtagaa cattccataa ataacgagtt ttatgataag
      301 ctctattttg actcaataat gtatgaattt cttctttccg gttacctttg tataactgca
      361 tcacagaaga gtagattctt tcgtgcgcaa atgactcatg tcgataatga gtgatggttc
      421 tacatgagtt cattaaggaa ttttctctat agttatgctt atacatataa tatggaaccg
      481 catacatatc gcatgtaatt agcagtcgat agataaaatc aagatcttct cctagtcgta
      541 aatcttcatc aaaaaaaaga ttattctctt ccagaagttg ttttcttata aacaagcacc
      601 ctatatggaa tctaaccttt ttataaaaaa agtcgaaaag taaagatcct tcattaaact
      661 ctgtatttat aggtgagtaa gtaacaccat cttttatata atggccataa agcccacaat
      721 aaatcgcatc ccctcccgtc tcatgttgtc tggtatacat cttcagcaga tagttaggat
      781 ctatctcatc atcatcatcc ataaaacata taaactgtcc ttgcgccttg cggatacctg
      841 tattcctggc agacgaaaca ccgccatttt ctttattgac aataattaat cgagagtggg
      901 ttaacccata ctctctcaaa acgtttgatg tgttatcttt agaaccatca ttaacaataa
      961 caatttcaaa atttgggtaa gtttgttttt ctaatttcgt tagagtttca gtaatgtaat
     1021 cagatgaatt atacgtcggt attattatag atactaaagg gtaatcattg ctcatcgtgt
     1081 tctcttgcct gaactaataa aatatgatag tactcatcct taattattat tgtaactcag
     1141 acatccatga tttttattat tatcc
//
LOCUS       DAZPEB010000380         1160 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZPEB010000380 DAZPEB010000000
VERSION     DAZPEB010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.386"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..925
                     /locus_tag="ACHURJ_004300"
     CDS             <1..925
                     /locus_tag="ACHURJ_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS110-like element ISEc11 family transposase"
                     /protein_id="HFR1282976.1"
                     /translation="RRQDFLTFFGNREPCLIGMEACGGSQHWARELTKLGHKVRLLQA
                     RFVKAFVMGNKNDVMDARAIWMAVQQPGKEIAVKTEEQQSVLVLHRTRMQLVKFRTAQ
                     INALHGTLLEFGETIHKGRAAMEREFPEALERMKERLPPYLIMVLENQYNRLNELDSL
                     IEDIEKQLTSVARQNETCKRLLDIPGVGPLIATAAVATMGEASAFKSGREFAAYVGLV
                     PKQTGSGGKVRLLGISKRGDTYLRTLFIHGARAVALVAKEPGPWITELKKRRPASVAI
                     VAMANKLARTVWAITAHDRKYDRNHVSIRPY"
BASE COUNT          324 a          262 c          333 g          241 t
ORIGIN      
        1 gcgtagacag gattttctga cgttcttcgg caaccgtgag ccatgcctga ttggtatgga
       61 ggcctgtgga ggttctcagc actgggcacg ggaactgaca aaacttggtc ataaagtccg
      121 gttgttgcag gcccgcttcg ttaaggcatt cgtcatgggc aataagaatg atgtgatgga
      181 tgcccgggct atctggatgg cggttcagca gccgggtaaa gaaatcgccg taaaaacaga
      241 agaacagcag tcggtactgg ttctgcaccg tacccgcatg caactggtga agttccggac
      301 cgcacaaatt aatgccctgc acgggacgtt actggagttt ggtgaaacca tccacaaagg
      361 ccgggcagcg atggagcggg agttccccga agcactggaa cggatgaaag agagactgcc
      421 accgtatctc attatggttc tggaaaacca gtacaaccga ctgaatgagc tggactcact
      481 gatagaggat attgaaaaac agcttaccag cgtggcgagg cagaatgaaa cctgtaagcg
      541 gttgctggat attcctggcg ttggaccact tattgcgacg gcagcggtgg ccaccatggg
      601 ggaagcatca gcgtttaaat cggggcgaga gttcgccgca tatgttggtc tggttccaaa
      661 acaaactggc tccggaggga aagtacgtct gctggggata agcaaacgtg gtgacactta
      721 tctcaggaca ttatttatcc acggtgcaag agcggtggca ttagtagcta aagagcctgg
      781 cccgtggata accgaactga aaaaacgtcg tccagccagt gtggcaatcg tcgccatggc
      841 aaacaagctg gcacgaacag tatgggcgat aaccgcccat gaccgtaagt atgacaggaa
      901 ccacgtcagt atcagaccat attaatcgct gataccatta aacaatgaac tcttaacaaa
      961 agggtgaatg ctgaaaggtt gctatggcgg ccagagtgat gacaaagaca ggtaagaccg
     1021 tgactcacta aacctgaaca gtattttggg cttgaagtcc gccgtgaaaa taaggggtga
     1081 gtcggcgaat tacatagggg ctcgcagcgt tacggctgca ataaagccgg atataaagct
     1141 gcaacctacc cgtcatgtca
//
LOCUS       DAZPEB010000381         1158 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZPEB010000381 DAZPEB010000000
VERSION     DAZPEB010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.387"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(1..264)
                     /locus_tag="ACHURJ_004301"
                     /pseudo
     CDS             complement(1..264)
                     /locus_tag="ACHURJ_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(379..1017)
                     /gene="ospI"
                     /locus_tag="ACHURJ_004302"
     CDS             complement(379..1017)
                     /gene="ospI"
                     /locus_tag="ACHURJ_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HFR1282977.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
BASE COUNT          339 a          286 c          176 g          357 t
ORIGIN      
        1 cagcaagcgc tgtaactctt ttatttgttt tatagcggca gccagttctg aggcaggaac
       61 gacatcctct ccggcagcaa cagcagtgag acttccttcc agatattatt ttttccactt
      121 aagcaacagg ctgggctgaa taccatgcaa acgcgccaca tgggacaccg tcatgccagg
      181 ctccattgtc tgctgaataa tggcaatttt ttcctgagga gttttacgtt tacgaagctc
      241 cagccccagc aaagttccag tcatctcaaa attagaacta gtgttagaca tatatccaag
      301 cctatctctt attttaagat acatccactg tctggcgttt caaggggccg gctcaaatct
      361 atgtacaggc tctcagcatc agcaaagcct cttactcttc caactactac tgttagcata
      421 gtgatcaaat tcctgatcag atacatgaat tacctgaacc gaactatttt catctacaaa
      481 tgcatctggc gatttttcta caggatcata aagattaaat ccctttcctg tttgaacatc
      541 caaaaacata attttttcac catcgttaaa tccagctatc caatgagtgg cctgttcaat
      601 agaaatcatt actacacttc cttttgaagt gttttgtaaa atattttctg caatacgctc
      661 acgaaattta ctataagttt cattaggatg tgacactatg ccttcactat caatttgctc
      721 taaatgccta tgaaaaaatc cggtcatata ctctgatatt tcattaggta cgggagcctc
      781 tcttactcca taccctaaca tagccataca agcatgaagc gcacaccctg aacagttacc
      841 atccccacca gacggattta tcattttctg taaagtcgct gccaattcag aatctttatc
      901 catatcacca agacttttag agtttaaaga ttccagcaat tttttaacat ttacatttcc
      961 ctcctctgta tgtgcctcat atccagcagt tccctgtaac gacaccccat taatcatatt
     1021 gtatcctgac agtatatata ctcactatat tttcttcata acaaatatcc ctctgatatg
     1081 acaacaaaaa acggagccgg attcctgttt ttgtgaactg cagctattcg agtctaaaaa
     1141 gtgatccact taccacca
//
LOCUS       DAZPEB010000382         1156 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZPEB010000382 DAZPEB010000000
VERSION     DAZPEB010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.388"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            397..>1156
                     /locus_tag="ACHURJ_004303"
     CDS             397..>1156
                     /locus_tag="ACHURJ_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1282978.1"
                     /translation="MSIDFTPGIINTYHGNIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQINNIRYEKNKSAECRIIVVANPKPDKAIITKISVEEGIPITFSV
                     QTMFSDTNFIAEQRADLPTNIKDIQSLYQKMTKLYIEHSENKNRMKVFAGTNFIDFNM
                     TGQNLSGFVLTLSRFYFEDLLNINFTDANLGDTIF"
BASE COUNT          433 a          218 c          167 g          338 t
ORIGIN      
        1 tatcggagaa gcaatgactc tttgtctcag taccctcctc ctttttaaaa ggcagatttc
       61 agtcgctaat acaacacatt tttaaataca ctttaacctg gttcattatt acacatctaa
      121 aacatcacaa aaatattact tcacacacta tcggagaaca taataacttt ttaaaatgag
      181 cgctttttga tagttgttaa cgttttcctt tacgatacta tcactgcttt ttttatacac
      241 aaatattacc gggtggaaat gaaagggaat ttaaaggtaa ttatatcagg aagatataac
      301 gcattatatt tattgtgtgt agaagaaaat ccattcctgt tctaattatc caatttaaaa
      361 accttagcat aaaacagatg atgaataagg aatgttatgt caattgactt taccccaggt
      421 ataataaata catatcacgg caatatttat aactgcacaa caaataccga taatgttaaa
      481 acgccagaca caccgaagtg gccttgtgat aattgggaag aacaacaacc cattaattcc
      541 acattctctg gagaaggata caaccctgaa caatttgatt tagcgcaaca acaactccaa
      601 caaattaatg catgccacac gaacaccaca tatacaaatg cagactactc aaaagttgtg
      661 gttcaacttg taagtcttat taataacatt gaaacgataa gttcgacacc acttacgcaa
      721 cagacacaat caatacttaa tcagattaat aacattagat atgaaaaaaa caaaagtgct
      781 gaatgtcgaa tcattgttgt cgctaatcct aaacccgaca aggcaatcat aactaaaatt
      841 agcgtagaag agggaatacc cataacattc agtgtccaaa ccatgttttc agatactaat
      901 tttattgctg aacaacgagc tgacttacca actaatataa aagatattca aagcctgtat
      961 caaaaaatga cgaagttata tattgagcac agtgaaaata aaaacaggat gaaagtcttt
     1021 gccggaacca actttattga tttcaatatg acaggacaga acctttccgg gttcgttcta
     1081 accttgtcaa ggttttattt cgaagatcta cttaatataa atttcactga cgcaaattta
     1141 ggtgacacta tttttt
//
LOCUS       DAZPEB010000383         1116 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZPEB010000383 DAZPEB010000000
VERSION     DAZPEB010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.389"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..803)
                     /locus_tag="ACHURJ_004304"
     CDS             complement(<1..803)
                     /locus_tag="ACHURJ_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR1282979.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSS"
BASE COUNT          322 a          157 c          264 g          373 t
ORIGIN      
        1 gaagaggtta atctttgcag ggattgcaga gcatgggatg acaatgggtt atcactaata
       61 tctattgaac attcattcct cagattaaga atgctttccg ggatatgact tatctgattt
      121 atgtcaaaat aaccgttcct taaattttcc ggcaggtatg gaatatcggt taacatattg
      181 tcgattgccc atatggagtg aatgttgtga ggtagagggg ggagttcact aagaagattc
      241 cctattgcgc taatctcttg taaagaaaat ggtaaaggtg gtaaaactgc cagcccatta
      301 catgatacat ctaatgtttc cagtagttct ggtaaaacag gaagagaaca taattgatta
      361 tttgagacat gaagctcctt caaagatatg gggagtgtgg gtagtgtgat tagttgattg
      421 tgggacgcat tcaataattt aagtccttga ggcaaagcag gcagttcaat aagtctgtta
      481 tagctgacat taagctgtgt aagggacgca ggcaatgggg agattaagct taaattattt
      541 ttacttatat taattgattt aattcccggg gggatttcag gtaatgttgt caggcctaat
      601 tcagacaagt ctaggttcgt ctcttggttt tgtagacatg atactagtcg ctgaaaagcg
      661 atgtctcgtt gttcttcttg tatgcggtta tttttccatt cagtccaatg ggttagataa
      721 ttttcatatg cgcggctagt gtcgattgaa taagtggaga acgaatttga aattaatctg
      781 tgattgttat ttgtcgggag cataaatatc agggcgtatt ctttatgttg agaggtgttg
      841 gattcttttt tatctgcttt gcaatgttat tggttccctt cagcagtaga caggaacttc
      901 tggaggaggc ggatatatag tttgtgtaag catattaact ccatatgtta tatattgaag
      961 aacttctgct ttacactatc ctaaactgga tcggtttaat gtaaaaaaac gatacttttt
     1021 gggggagggg gcaatttttc cttaaggtat gaaaaaagga gctgaagcta tataatagct
     1081 attaatgcca ctggtatcag tgaatcatgt atataa
//
LOCUS       DAZPEB010000384         1107 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZPEB010000384 DAZPEB010000000
VERSION     DAZPEB010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.390"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..544
                     /locus_tag="ACHURJ_004305"
     CDS             <1..544
                     /locus_tag="ACHURJ_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HFR1282980.1"
                     /translation="QIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLYYLNQ
                     HFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAANELT
                     SALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISDLKQI
                     VPRSAESQQRDKVATFPKLA"
     gene            complement(551..979)
                     /gene="uspC"
                     /locus_tag="ACHURJ_004306"
     CDS             complement(551..979)
                     /gene="uspC"
                     /locus_tag="ACHURJ_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFR1282981.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
BASE COUNT          305 a          254 c          258 g          290 t
ORIGIN      
        1 ccagattgca ccaacgtcgc gtggtgatgt gcaagcctat caggatattc gtcatcagct
       61 cgaaaatgaa gctggacgaa ttaatggtaa atacgggcaa ttaggctgga cgccgcttta
      121 ttatttgaat cagcattttg accgtaaatt actgatgaaa atattccgct actctgacgt
      181 gggcttagtg acgccactgc gtgacgggat gaacctggta gcaaaagagt atgttgctgc
      241 tcaggaccca gccaacccgg gcgttcttgt tctttcgcaa tttgcgggag cggcaaacga
      301 gttaacgtcg gcgttaattg ttaaccccta cgatcgtgac gaagttgcag ctgcgctgga
      361 tcgtgcattg actatgtcgc tggcggaacg tatttcccgt catgcagaaa tgctggacgt
      421 tatcgtgaaa aacgatatta accactggca ggagtgcttc attagcgacc taaagcagat
      481 agttccgcga agcgcggaaa gccagcagcg cgataaagtt gctacctttc caaagcttgc
      541 gtaggagcgg ttaatcaccc gtaagtggaa ctaacagcac atcgacctca cttgaggcaa
      601 tcactctttt cgccgagcag gatgctcgcg aaaagaaact gtgattgtga ttaccgcaaa
      661 tcaccaaatc gaaatgatgt ttgtgacata cttccagaat atgttcgctt aactcaccgt
      721 aggcaataaa cgtcttgtca acgggatacc ccgcatcctg aattaactta tcaagaaagc
      781 tttgcgtttc ttcttgcatc acgctgcgta aatcttcaag catcggcgcg gctaattgat
      841 tgtacatttc cgggtcggaa gcgagagtaa ttaaactgat gtgtcccttt actggcctgg
      901 cgatagatac cgcttttgcc agcagttgct gactttccgg tgtaaccgca acagcgacaa
      961 gaatattgct atagctcata acctgttcct tattctgtga acttcaggtg acattaaagc
     1021 tttactccta atggagaaac gacgcaatcc caactcggtc aaacaggtaa atattagtct
     1081 gattattagt aaagacaatt aatcaat
//
LOCUS       DAZPEB010000385         1100 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZPEB010000385 DAZPEB010000000
VERSION     DAZPEB010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.391"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..174)
                     /locus_tag="ACHURJ_004307"
     CDS             complement(<1..174)
                     /locus_tag="ACHURJ_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory CII family protein"
                     /protein_id="HFR1282982.1"
                     /translation="MFDYQVSKHPHFDEACRAFALRHNLVQLAERAGMNVQILRNKLN
                     PAQPHLLTAPEIWL"
     gene            complement(207..428)
                     /locus_tag="ACHURJ_004308"
     CDS             complement(207..428)
                     /locus_tag="ACHURJ_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HFR1282983.1"
                     /translation="MRPNISITLTTPHVTIERYSELTGLSIDTINDMLADGRLIRHRL
                     RKDKKREKVMINIAAMTVDALSECNLNLN"
     gene            554..>1100
                     /locus_tag="ACHURJ_004309"
     CDS             554..>1100
                     /locus_tag="ACHURJ_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein CI"
                     /protein_id="HFR1282984.1"
                     /translation="MNLEKGGRGAIERMVEAYGFKTRQALCDHLGISKSTLATRYMRD
                     SFPAEWVIQCALETGTSLNWLTTGHGSKQTSGNTNTMEVAKYVLSDGALREDGFYIFD
                     KGFLPSTFKKPFVITDNNSEFICDKEFDDIRDGKWVISIDGEVTIRDITRLPGGRIFV
                     EGGNRAFECKIEDIEIIGKIIS"
BASE COUNT          320 a          204 c          258 g          318 t
ORIGIN      
        1 cagccagatt tctggtgcgg ttaataaatg aggctgagct gggttcagct tgttccgcag
       61 aatctgcaca ttcatgcctg cacgttctgc cagttgcacc agattgtggc gcagtgcaaa
      121 tgcacgacag gcttcatcaa aatgtggatg tttggaaact tggtaatcaa acatggtcaa
      181 tgcctctgat gtatttcaga atcgaactaa ttaaggttta gattgcattc tgaaagcgca
      241 tcaacggtca ttgctgctat gttgatcatc actttttcgc gttttttatc tttgcgcaga
      301 cggtgacgga taaggcgtcc atcagccaac atgtcattga tggtatcgat ggatagccct
      361 gtcagctcgc tatagcgttc aatagtcaca tgaggcgtgg taagagtgat tgaaatgtta
      421 ggtctcatga tgcaacattc ctcgtttaat gatgattaat caggacgaat acggatcgtt
      481 tgtattttgt gaacaccata aacatatgat tgcacagtga aatcgtcaag ataaaagttc
      541 acttggagtg accatgaatt tggagaaagg cggacgaggc gccatagagc gcatggtaga
      601 agcttatgga ttcaagactc gacaggcgtt gtgcgatcat ttaggaatct ctaaaagtac
      661 actcgccaca cgctacatgc gtgactcatt cccagcagaa tgggtaatcc agtgcgccct
      721 tgaaacgggc acctcgctta attggctcac aaccggacat ggttcaaagc aaacttcagg
      781 taatacaaat actatggaag ttgctaaata tgtattatct gatggtgcct tgcgtgaaga
      841 cggtttttat atttttgata agggatttct accctctacg tttaaaaaac cttttgtcat
      901 cacagataac aattctgaat ttatttgtga taaagaattt gatgacatac gtgatggtaa
      961 atgggtaata agtattgatg gcgaagtaac gatccgtgac attactcgtt tacccggtgg
     1021 aagaatcttc gtcgagggtg gaaacagagc cttcgaatgt aagatagaag acattgaaat
     1081 aattggtaaa attataagtt
//
LOCUS       DAZPEB010000386         1096 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZPEB010000386 DAZPEB010000000
VERSION     DAZPEB010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.392"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..211
                     /locus_tag="ACHURJ_004310"
     CDS             <1..211
                     /locus_tag="ACHURJ_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HFR1282985.1"
                     /translation="YDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDN
                     AIKYENAEMIKLLLKYGATSDNKYI"
     gene            complement(315..463)
                     /locus_tag="ACHURJ_004311"
                     /pseudo
     CDS             complement(315..463)
                     /locus_tag="ACHURJ_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            539..>1096
                     /gene="ospD3"
                     /locus_tag="ACHURJ_004312"
     CDS             539..>1096
                     /gene="ospD3"
                     /locus_tag="ACHURJ_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HFR1282986.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGKLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKF"
BASE COUNT          380 a          180 c          207 g          329 t
ORIGIN      
        1 gtatgatatt gagtatctac ttagcgctca taattcaagc tgtaaagtac ttgagtattt
       61 tatcaataag gggttggttg atgtaaacac aaagttcaaa aaaactaata gtggggattg
      121 tatgttggat aacgcaataa aatatgagaa tgcagaaatg ataaaactat tattgaaata
      181 tggtgcaaca tctgacaata aatatattta atcaaaattg aatatcgttt agcaaaaggc
      241 agtatttgca caaaatatcg atatattgtt tattgtcagt atggctcaat gtgataatag
      301 ttggaaagtt tgatgggttt cgccccgttg tagcggtagt cgagctggaa ggtcttcagg
      361 cactgcttac agcgatagag cagcccccct gaactggaat ggccgttccg gataccccct
      421 gagtttcaga gtaacgggga caaaccacat caatctttgc catcaatcat ccaaagggca
      481 aagagtacaa caacactaag tctgcgtcac aacccatcaa tgaaaggaat atatacatat
      541 gccatcagta aatttaatcc catcaaggaa aatatgtttg caaaatatga taaataaaga
      601 caacgtctct gttgagacaa tccagtctct attgcactca aaacaattgc catatttttc
      661 tgacaagagg agttttttat taaatctaaa ttgccaagtt accgatcact ctggaaaact
      721 tattgtctgt cgacacttag cttcctactg gatagcacag tttaacaaaa gtagtggtca
      781 cgtggattat catcactttg cttttccgga tgaaattaaa aattatgttt cagtgagtga
      841 agaagaaaag gctattaatg tgcctgctat tatttatttt gttgaaaacg gttcatgggg
      901 agatattatt ttttatattt tcaatgaaat gatttttcat tccgaaaaaa gcagagcact
      961 agaaataagt acatcaaatc acaatatggc attaggcttg aagattaaag aaactaaaaa
     1021 tgggggggat tttgtcattc agctttatga tcccaaccat acagcaactc atttacgagc
     1081 agagtttaac aaattt
//
LOCUS       DAZPEB010000387         1091 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZPEB010000387 DAZPEB010000000
VERSION     DAZPEB010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.393"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..>1091
                     /locus_tag="ACHURJ_004313"
     CDS             <1..>1091
                     /locus_tag="ACHURJ_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFR1282987.1"
                     /translation="YATDPAGNRLPDPELHPDSTLSMWPDNRIARDAHYLYRYDRHGR
                     LTEKTDLIPEGGIRTDDERTHWYHYDSQHRLVHYTRTQYAEPLVESRYLYDPLGRRVA
                     KRVWRRERDLTGWMSLSRKPEVTWYGWDGDRLTTIQNDRTRIQTVYQPGSFTPLIRVE
                     TATGELAKTQRRSLADALQQSGGEDGGSVVFPPVLVQMLDRLESEILADRVSEESRRW
                     LASCGLTVAQMKNQMDPVYTPARKIHLYHCDHRGLPLVLISTEGATEWCAEYDEWGNL
                     LNEENPHHLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNFYQY
                     PLSPVNSMDPLGLYEFKSKNIDDIGIFAL"
BASE COUNT          261 a          302 c          336 g          192 t
ORIGIN      
        1 gtatgccaca gacccggcag gtaaccgcct gcccgacccg gagctgcacc cggacagcac
       61 cctcagcatg tggccggata accgtatcgc ccgtgacgcg cactatcttt accggtatga
      121 ccgtcacggc aggctgacag agaaaaccga cctcatcccg gaagggggta tccgcacgga
      181 tgatgagcgc acccactggt accattacga cagtcagcac cggctggtgc actacacgcg
      241 gacacaatat gcagagccgc tggtcgaaag tcgctatctt tacgacccgc tgggccgcag
      301 ggtggcaaaa cgggtatggc ggcgtgaacg ggacctgacg ggctggatgt cgctgtcacg
      361 gaaaccggaa gtgacctggt acggctggga cggcgaccgg ctgaccacaa tacagaacga
      421 cagaacccgc atccagacgg tttatcagcc gggaagcttc acgccactca tcagagttga
      481 aaccgccacc ggtgagctgg cgaaaacgca gcgccgcagc ctggcggatg cccttcagca
      541 gtccggcggc gaagacggtg gcagtgtggt gttcccgccg gtgctggtgc agatgctcga
      601 ccggctggaa agtgaaatcc tggctgaccg ggtgagtgag gaaagccgcc gctggctggc
      661 atcgtgcggc ctgaccgtgg cgcagatgaa aaaccagatg gacccggtgt acacgccggc
      721 gcgaaaaatt cacctgtacc actgcgacca tcgcggcctg ccgctggtgc ttatcagcac
      781 ggaaggggca acagaatggt gcgcagaata cgatgagtgg ggcaacctgc tgaatgaaga
      841 gaacccgcat catctgcagc agctcattcg cctgccgggg cagcagtatg atgaggagtc
      901 cggcctgtat tacaaccgcc accgctatta tgacccgctg caggggcgat atatcactca
      961 ggatccgatt gggctgaagg ggggatggaa tttttatcag tatccgctga gcccggtgaa
     1021 tagcatggat ccattaggat tatatgaatt taaatcaaaa aatatagatg atattggaat
     1081 atttgcattg g
//
LOCUS       DAZPEB010000388         1083 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZPEB010000388 DAZPEB010000000
VERSION     DAZPEB010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.394"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..474)
                     /gene="mxiJ"
                     /locus_tag="ACHURJ_004314"
     CDS             complement(<1..474)
                     /gene="mxiJ"
                     /locus_tag="ACHURJ_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HFR1282988.1"
                     /translation="MIRYKCFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKE"
     gene            complement(480..773)
                     /gene="mxiI"
                     /locus_tag="ACHURJ_004315"
     CDS             complement(480..773)
                     /gene="mxiI"
                     /locus_tag="ACHURJ_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HFR1282989.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSHPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            complement(786..1037)
                     /locus_tag="ACHURJ_004316"
     CDS             complement(786..1037)
                     /locus_tag="ACHURJ_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
                     /protein_id="HFR1282990.1"
                     /translation="MSVTVPDKDWTLSSLSETFDDGTQTLQGQLTSALNALAENPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
BASE COUNT          327 a          217 c          167 g          372 t
ORIGIN      
        1 ctctttcggt gagtcatata tagcgattac tgatatatgc atcggttttg aagatatatt
       61 tttttcttca agatcatagc taacatgtat ttttgccgaa ataacaccac caatagaaac
      121 taaagactgt tccagccgtt gctcaatagc actatataaa cgggcctttt cagctcttgg
      181 agaagacact aatgaatctg taggaaacat ttgtgagata tcaactctct ccggatttgg
      241 caaatcgtac atgcgcatca aatcaactgc cgatgcaaat gtcccctttt cgacttgtac
      301 cgagatcccc tgtttacctc catcaacttt tctagcagta atattatggc gttctagcac
      361 agatattatt tcatttgcct gtctttgaga taaattagaa attaactctt cacgttgctc
      421 acatccaatc aacatcagca acaagaataa aataaaacat ttatacctaa tcatataaat
      481 taagacttta ataaagtttc aacagccgaa actgtttttc ttgctaacgt gccagctaat
      541 gatactccta ttgaataatt tgagagcgtc gtctgtaact tggccaaaga ttctgggttt
      601 aatgattctg gatggcttac catctccatt atttgtgata cttgaatatc tgtatccatc
      661 ttaatatcac tatatttagc cgacacgacg tcttccaggc ttgatatctc ttgagattga
      721 aaatcactgg ctttgataat atcaacctga ttgactggat aaatgtaatt catgaatgct
      781 ccctattatc tgaagttttg aataattgca gcatcaacat ccttaatcac tttcacagta
      841 ttagattgcg cgttcctata taatgtatat tcagataatt tactttggta ttcagccagc
      901 aactgtggat tcgaaggatt ttcagctaat gcatttagtg ccgatgttag ttgcccttgt
      961 aatgtttgag ttccatcatc aaaagtttca gataatgaac tcagagtcca atctttatcc
     1021 ggtactgtaa cactcatttt atcctcactt atttttatct aaaaaaaacc agtgtttatc
     1081 att
//
LOCUS       DAZPEB010000389         1057 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZPEB010000389 DAZPEB010000000
VERSION     DAZPEB010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.395"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..842
                     /locus_tag="ACHURJ_004317"
     CDS             <1..842
                     /locus_tag="ACHURJ_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFR1282991.1"
                     /translation="LVGYIAWLATAYRMSFSREINLGLAQHEFELFCQPLLNARSQQC
                     IGVEILLRWNNPRQGRISPDVFIPIAEEHHLIVPLTRYVMAETIRQRHVFPMSSQFHV
                     GINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITERDALLDVDYRIARELHRKNVK
                     LAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSDAVNSTVTDIIIALGQRLNIEL
                     VAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFPKWLAGSQPPPARHNGHITPIM
                     PLR"
BASE COUNT          241 a          268 c          268 g          280 t
ORIGIN      
        1 tactggtggg ctacatcgcc tggctggcga ccgcttaccg gatgagcttt tcccgcgaaa
       61 tcaatctggg cctggcgcaa catgagttcg aattgttctg tcagcctttg cttaatgcgc
      121 gcagccagca atgtattggt gtagagattt tgctgcgctg gaacaatccg cgtcagggcc
      181 ggatttcacc ggatgtgttt attcctatcg cggaagaaca tcatttaatt gtgccactga
      241 cccgctatgt gatggcagaa accattcgtc agcgccatgt tttcccgatg agtagtcagt
      301 ttcatgttgg cattaacgtc gcacccagcc attttcgccg tggtgtgctg ataaaagatc
      361 tcaatcagta ctggtttagc gctcacccga ttcagcaact gatcctcgaa atcaccgaac
      421 gcgatgcctt actggatgtt gattatcgga ttgcccgcga gctacatcgt aaaaacgtca
      481 aactggcgat tgatgacttc ggcaccggca acagttcatt ttcctggctt gaaacattac
      541 gtcctgacgt gctgaaaatt gataagtcat ttaccgcagc tataggttct gacgcggtta
      601 actcgacggt gaccgatatc atcatcgcgc tggggcaaag actgaatatt gaactggtgg
      661 cggagggcgt ggaaacgcaa gaacaggcga agtatttgcg ccgtcatggc gtgcatattt
      721 tgcaagggta tttgtacgca cagccgatgc cgctacgcga ttttcccaaa tggctggcgg
      781 gcagccaacc gccgcccgcc cggcataatg gacatatcac gcccattatg ccgttacgtt
      841 aacgctgata tgactttccg tgtcaaaccg gaatctctaa ttttgacgat tttcttttta
      901 ggtttaattc cctgagggcg acgtagcagt aaatccgacg gtgaatcacg ctgatattcg
      961 cgggttggat accgcattac agaggtccgt catatgagcc tgccactttc ttacgtcgcg
     1021 aatcagcttt gttccgccgt cgccaagcgc taacgtg
//
LOCUS       DAZPEB010000390         1056 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZPEB010000390 DAZPEB010000000
VERSION     DAZPEB010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.396"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..1033
                     /locus_tag="ACHURJ_004318"
     rRNA            <1..1033
                     /locus_tag="ACHURJ_004318"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          261 a          247 c          330 g          218 t
ORIGIN      
        1 actccgtgcc agcagccgcg gtaatacgga gggtgcaagc gttaatcgga attactgggc
       61 gtaaagcgca cgcaggcggt ttgttaagtc agatgtgaaa tccccgggct caacctggga
      121 actgcatctg atactggcaa gcttgagtct cgtagagggg ggtagaattc caggtgtagc
      181 ggtgaaatgc gtagagatct ggaggaatac cggtggcgaa ggcggccccc tggacgaaga
      241 ctgacgctca ggtgcgaaag cgtggggagc aaacaggatt agataccctg gtagtccacg
      301 ccgtaaacga tgtcgacttg gaggttgtgc ccttgaggcg tggcttccgg agctaacgcg
      361 ttaagtcgac cgcctgggga gtacggccgc aaggttaaaa ctcaaatgaa ttgacggggg
      421 cccgcacaag cggtggagca tgtggtttaa ttcgatgcaa cgcgaagaac cttacctggt
      481 cttgacatcc acggaagttt tcagagatga gaatgtgcct tcgggaaccg tgagacaggt
      541 gctgcatggc tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc
      601 aacccttatc ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata
      661 aactggagga aggtggggac gacgtcaagt catcatggcc cttacgacca gggctacaca
      721 cgtgctacaa tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa
      781 gtgcgtcgta gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta
      841 atcgtggatc agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac
      901 accatgggag tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac
      961 tttgtgattc atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg
     1021 gatcacctcc ttaccttaaa gaagcgtact ttgcag
//
LOCUS       DAZPEB010000391         1049 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZPEB010000391 DAZPEB010000000
VERSION     DAZPEB010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.397"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            192..1049
                     /locus_tag="ACHURJ_004319"
                     /pseudo
     CDS             192..1049
                     /locus_tag="ACHURJ_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544402.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          309 a          240 c          246 g          254 t
ORIGIN      
        1 acccttttgc ttttatatct gcgtctaaaa taaaaatgag ccgcaggtta accgcaaaag
       61 ttacatgctc acatagcaaa aagaatagcc aacttcatta tggcttcagt gagatgtatg
      121 gtcgtaggat ttcatacatt gacactggtt atacatacag taaaaatact ctctactgga
      181 gggcattttt tatggcagta cgaaaactca ccactgggaa atggctttgc gaatgttacc
      241 ccgccggacg aagtgggcgt cgtgtgcgta aacaattcgc caccaaaggc gaagcactgg
      301 cttttgagcg ccacacgatg gaagaaaccg aagcaaagcc ctggctgggt gaatcagtgg
      361 atcgtcgaac actgaaagac gtggttgagc tatggttcaa actacatggt aaatctctga
      421 cagctgggca gcatgtctat gacaaattgc tgttgatggt tgacgctctg ggcaatcctc
      481 ttgcaaccga tctcacctct aaaatgtttg cccactatcg agataaacgc ctgacaggcg
      541 ggatctactt cagcgagaaa tggaagaaag gagcaagccc ggtcaccatt aacctggagc
      601 aaagctatct aagtagtgtt tttagcgaac tatcccgtct gggcgaatgg tcgtatccga
      661 acccactgga gaacatgcga aaattcacca tcgcagaaaa agagatggca tggcttaccc
      721 atgagcagat tgttgaattg ctggctgatt gcaaacgtca ggacccaatt ctggcactgg
      781 tagttaagat atgcttaagc acaggcgcac gctagcgtga agccgtaaat cttacccgct
      841 cacaggtgac caaataccga attacctttg tcagaacgaa ggggaagaaa aacagaagca
      901 tccctatcag taaagagctt tacgaagaga tcatggcgct tgatgggttc aatttcttca
      961 cagactgcta ttttcaattt ttatccgtga tggaaaaaac gtctatcgtg ctccctcgcg
     1021 gtcaactcac acacgttctg cgccatacg
//
LOCUS       DAZPEB010000392         1044 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZPEB010000392 DAZPEB010000000
VERSION     DAZPEB010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.398"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..510
                     /locus_tag="ACHURJ_004320"
     CDS             <1..510
                     /locus_tag="ACHURJ_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFR1282992.1"
                     /translation="LSAGYLQPHTDMPGSDDLKGINVKYRYEFTDTLGLVTSFSYANA
                     EDEQKTHYSDTRWHEDSVRNRWFSVMAGPSVRVNEWFSAYAMAGVAYSRVSTFSGDYL
                     RVTDNKGKTHDVLTGSDDNRHSNTSLAWGAGVQFNPTESVAIDLAYEGSGSGDWRTDG
                     FIVGVGYKF"
     gene            574..>1044
                     /locus_tag="ACHURJ_004321"
     CDS             574..>1044
                     /locus_tag="ACHURJ_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR1282993.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     MKRFEEMVVQAQQSAGAAAGNAQQTAQDVAAAATARDDAQRFAENARQDATVTAE"
BASE COUNT          233 a          245 c          344 g          222 t
ORIGIN      
        1 ctgagcgccg ggtatcttca gccccatact gatatgccag gcagcgatga cctgaagggc
       61 attaacgtga aataccgtta tgagtttacg gacacgttgg ggctggtgac gtcattcagc
      121 tatgccaacg ctgaagatga gcaaaaaacg cattacagcg atacccgctg gcatgaggat
      181 tccgtgcgta accgctggtt cagcgtgatg gcggggccgt ctgtgcgcgt gaatgaatgg
      241 ttcagcgcgt atgcgatggc gggcgtggct tacagccgtg tgtcgacctt ctccggggat
      301 tatctccgcg taactgacaa caaggggaaa acgcacgatg tgctgaccgg gagtgatgac
      361 aaccgccaca gcaacacgtc tctggcgtgg ggggctggcg tgcagtttaa cccgaccgaa
      421 tccgtggcca ttgaccttgc ttatgaaggt tccggcagtg gcgactggcg cactgacggt
      481 ttcatcgtgg gtgtcggtta taagttctga ttagccaggt aacacagtgt tatgacagcc
      541 cgctggttca ggcgggcttt ttgtggggtg aatatggcag taaagatttc aggtgtactg
      601 aaagatgggg cgggtaaacc tgtcgtaaat tgtgcgattg aactgcgggc cagaagaacc
      661 agtccgaccg ttgtggcaca cgttgttgcc acttgcgtga cggacaataa cggtgcttat
      721 gtgattgagg ctgagccggg gtattacgag gttgcgcttc actgtaacgg ctggcagcca
      781 acccgtgtcg gggatattga tgtggcaccg actgatgcac cggggacact gaacgcgttt
      841 ctgaatgcac caaaggatgg tgatttacgt ccggaggtga tgaagcgctt tgaggaaatg
      901 gtggtgcagg cgcagcagag cgccggggcc gcagccggaa acgcacagca gacggcgcag
      961 gatgtggcgg cagccgcaac ggcccgtgat gatgcacaac gttttgcgga gaatgccaga
     1021 caggatgcaa ccgtcacagc tgag
//
LOCUS       DAZPEB010000393         1044 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZPEB010000393 DAZPEB010000000
VERSION     DAZPEB010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.399"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(1..1044)
                     /locus_tag="ACHURJ_004322"
                     /pseudo
     CDS             complement(1..1044)
                     /locus_tag="ACHURJ_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591423.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
BASE COUNT          261 a          277 c          247 g          259 t
ORIGIN      
        1 ctggccagtt tttttcaaag taaatacctt taatacacgg ttgcatacgt aaagacttca
       61 cttcggtaat aaaagaacct tgcgaatatt gaatcgttaa tctgtgattt ttgtcttcag
      121 cacgttcaga gcgaaaacgc cattcttctt ttgcgccgcg acgagtttgt gggaggcatt
      181 tgccttgtgg atcaataacc atccgtgctc cacgactgcc accacctgcg tcaatataat
      241 gcgtcaattg cgttaatacg gcggcagaag tcagcgtcat atgccgccac ataaacagtt
      301 cggcaatttc gcctgcgtga ttgatggtta aaccgtgttg ttgtacaaac tcacttagca
      361 gcagggcgtc acgggtggca tgacgaactt tatcggcatg acaaataaat ccggcgtaat
      421 cagacattcg cgcctggatt ttgtctttca ccgttaccag cgccattccg ttctcgtttg
      481 ccagtgcctg agcgataact tctctgacta tattcagaga tgaggctacc agctgcgcga
      541 catcaccctc aacagaccgc ttttgcgtgc aaccaataaa acgtgccaga cgaacagcaa
      601 aaacctgccc ggcattcaat gctgcaccac tagggcgagt gacgccgtgt gtgccagcaa
      661 cttcccccac ggcgaaacag ccgggcaggg aggtttgtcc ccagatatcc acttcaatgc
      721 cgccattcat atgctgatta ttcatggcaa actgcaaagg ctgtgtggtg agatcgtaac
      781 cgtgcatctt atacagcgag atagacagcg gattcattcg ttgcagccgt tcgatgggcg
      841 atggtgccag agcgtcgtta ttttccagat acgcgcgaac gtcgtcgtcc agtcgatcta
      901 atgagaacgg caggtcaccc ggcaccgctt caggattgcg attgaaatcc ataaatacct
      961 gacgccctga ttgctgctct tgcgctactg ccatatctaa caagcggagc caaaatccat
     1021 cacccgagtg gcgtggaacg gcca
//
LOCUS       DAZPEB010000394         1036 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZPEB010000394 DAZPEB010000000
VERSION     DAZPEB010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.400"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..975)
                     /locus_tag="ACHURJ_004323"
     CDS             complement(<1..975)
                     /locus_tag="ACHURJ_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HFR1282994.1"
                     /translation="MIRKSATGVIVALAVIWGGGIWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSHFQMQISFDNGAPDLNIKPGQKVAFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLTRLE
                     EKKFPVGESESKFNQVNIINQGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEV"
BASE COUNT          299 a          232 c          186 g          319 t
ORIGIN      
        1 aacttcatcg tttgctaact cagggtgtgc agcaagctgc ttctgcatcg caatgttata
       61 ctggatgata aattggcgca ctgctgaggg atcaatagat tcagcaatca atgaccactc
      121 accactaccg agttgctgat tcccttttgt taacttatca agttcgtagg tcagattgac
      181 attgatataa tctttgtctt taacgcgatc cagcctggtt tttgcaacga aagcatcgat
      241 ttgggcaacg tcttccccct gattgataat attaacctga ttaaatttcg actcgctttc
      301 accaaccgga aatttctttt cttctaatcg tgtcagagaa tcaatggtaa aactttttgc
      361 cgttactttg ttcattggtg ataattgcag aacaatatct tcgactttgc cttcaatatc
      421 caggttagac aatgaactac catcaccatt aaaagtaaac tggccctcac caaaagaaaa
      481 ctttccatac tctgcagggg caacatctaa tgtcgtagag aatgagccac caaacgcgaa
      541 tcgcaatgtc gcttccacgg gcgatttatt tttcgcggcg ataaatagcg gttgtgtcag
      601 ttcattattc actaagttca cttttgccgc tgccagtgct gggatgacat taccatgcat
      661 taacattgtg atgggcaacg gaccgtgctc aacatccaca tcaaatgcaa ctttctggcc
      721 tggcttgata ttgagatcgg gtgcaccgtt atcaaatgaa atttgcattt gaaaatggga
      781 attaaaaaag cctttgtcaa agtttttata acttaacgtc atatcgtagg catgttcacc
      841 tttcttttta gcatcgttaa aatctttaat aaatttttcg acaccaggct gaatttgcgt
      901 acctgtgtac catatgccac caccccagat tacggctaac gcaacaataa cacctgtagc
      961 tgacttacgt atcatataaa tattccctga taaaacagca aaactttgaa tatccactta
     1021 tgctgaaata atgtcc
//
LOCUS       DAZPEB010000395         1024 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZPEB010000395 DAZPEB010000000
VERSION     DAZPEB010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.401"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            159..809
                     /gene="csgD"
                     /locus_tag="ACHURJ_004324"
     CDS             159..809
                     /gene="csgD"
                     /locus_tag="ACHURJ_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFR1282995.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYSMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            814..933
                     /locus_tag="ACHURJ_004325"
                     /pseudo
     CDS             814..933
                     /locus_tag="ACHURJ_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="chaperone-like protein that participates in the
                     polymerization of curlin (CsgA) subunits into curli
                     (extracellular fibers from Escherichia and Salmonella spp.
                     that are involved in the colonization of inert surfaces
                     and biofilm formation); part of the curli secretion and
                     assembly protein complex; incomplete; partial in the
                     middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgE"
BASE COUNT          289 a          217 c          216 g          302 t
ORIGIN      
        1 agattgaaat cagatgtaat ccattagttt tatattttac ccatttaggg ctgatttatt
       61 actacacaca gcagtgcaac atctgtcagt acttctggtg cttctatttt agaggcagct
      121 gtcaggtgtg cgatcaataa aaaaagcggg gtttcatcat gtttaatgaa gtccatagta
      181 ttcatggtca tacattattg ttgatcacta aaccttcttt gcaggcgaca gctctcttgc
      241 agcaccttaa acaatcgctg gcaattacag gaaaattaca taatattcaa cgttctctgg
      301 acgatatctc ttcaggctct attattcttc tggatatgat ggaagcagat aaaaagctta
      361 tccattattg gcaggatact ttgagcagga aaaacaacaa tatcaaaata ttgttgctaa
      421 atacgcctga agattacccg taccgcgaca ttgaaaactg gcctcatatc aacggcgttt
      481 tttattccat ggaggatcaa gaacgtgttg tcaatgggtt gcaaggcgtc ctgcgcggcg
      541 aatgctactt tacgcaaaag cttgccagct acttgattac gcattcaggt aactatcgtt
      601 ataacagcac ggaatcagcc ctccttactc atcgggaaaa agagatcctg aataagctgc
      661 gtatcggcgc gtctaataac gagatcgctc gttcgttgtt catcagcgaa aatacggtta
      721 aaacgcatct ttataatctt ttcaagaaga tagccgtaaa aaaccggaca caagcggttt
      781 cctgggcaaa cgataacctc aggcgataaa gccatgaaac gttatttacg ctggattgtg
      841 gcggcagaat ttctgttcgc cgcagggaat cttcacgccg ttgaggtaga agtcccggga
      901 ttgctaactg accatactgt ttcatctatt ggccggtaat gctgccaact tactgattta
      961 gtgtatgatg gtgattttaa ggtgcttgcg tggcttccat ttccatcaga tgtccttcct
     1021 gctc
//
LOCUS       DAZPEB010000396         1018 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZPEB010000396 DAZPEB010000000
VERSION     DAZPEB010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.402"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..767)
                     /gene="ipaH3"
                     /locus_tag="ACHURJ_004326"
     CDS             complement(<1..767)
                     /gene="ipaH3"
                     /locus_tag="ACHURJ_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH3/Hc"
                     /protein_id="HFR1282996.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNHLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITHIPENILSLDPTCTIILEDNPLSSRIRESLSQQTA"
BASE COUNT          289 a          163 c          249 g          317 t
ORIGIN      
        1 tgggcggttt gttgcgacag agactccctg atccgtgagg acagaggatt gtcttcgagg
       61 atgatagtgc aggtcggatc aaggctaagt atattttccg gaatgtgtgt gatgcgattc
      121 tcgcggcacc ggaaaaatat ctcggtttcc tctgagtgat gatttcttac aggtacggct
      181 ggtaggcttt ccagaagatt agtacttaca tcgagcgctt ccagtgattc aggtaactca
      241 gggagaaatg tcagctggtt atttcttact gagagcactt ccagcgatgt aggtaattca
      301 ggaagcatgg ttagctgatt gttatctgca ttaatatatt ccagcaatgc aggcaattca
      361 ggaagcatgg ttagttggtt gttatctaca tcaagatgtt tcagagatgc gggtaattca
      421 ggaagtgttg acaggtgatt gtcacaggcg tcaaggtatt ccagcgatgc tggcaattct
      481 ggtaatgata ttagggcatt ctgagtaatt tccagaacag tgatttgagg tggtaagttg
      541 tcaggtagcg aggacagatt taaacgattc agttgaagct cactgaactg attgatgaga
      601 cattctttaa gtagggagac cgcttcattc cgattttcac cggggagcgc ttgtttttcc
      661 catttatccc atgctgaaaa gtaatcagca tatgtaccgg aaatagtgtt ataaaaagaa
      721 ttttgggaca atgaaaagtt attatttatc ggtaacatga tggacattca gttaaagccg
      781 atggacaaat ggctttgtta aaagaaataa gtaggagttt ttatgaagtt cattccatga
      841 aatgaacttc agcacttttt tattataaaa ggacaaacaa aattggctgt aaaaaaacga
      901 tactgtttca gttaactgtg aatagcttgc ttgcaccgta ttgttcttgt ttttcggtta
      961 gttaaacatc gtacttcata tttgaacgtt ctgccggaat gcattatcaa tagaggta
//
LOCUS       DAZPEB010000397         1010 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZPEB010000397 DAZPEB010000000
VERSION     DAZPEB010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.403"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            201..395
                     /gene="bfd"
                     /locus_tag="ACHURJ_004327"
     CDS             201..395
                     /gene="bfd"
                     /locus_tag="ACHURJ_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFR1282997.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            468..944
                     /gene="bfr"
                     /locus_tag="ACHURJ_004328"
     CDS             468..944
                     /gene="bfr"
                     /locus_tag="ACHURJ_004328"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFR1282998.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGTPNLQDLGKLGIGEDVEEMLQSDLRLEQEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(941..>1010)
                     /locus_tag="ACHURJ_004329"
     CDS             complement(941..>1010)
                     /locus_tag="ACHURJ_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFR1282999.1"
                     /translation="PFGPFLAAAGFVIGWDSLLIGR"
BASE COUNT          302 a          206 c          231 g          271 t
ORIGIN      
        1 gctaaagttc tgggttaatt acacgttaat tagttttgaa ttgaaaaggg cgcttcggcg
       61 ccctttttgc tgcccgtaat ttgagctcga aatatttagt cgtaattttg ctcgctgaac
      121 tatggcgcaa ataaaactgt gctattgtaa tcataatcat tctcatttac actttgcgca
      181 gaatttgaac gggagcaatc atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa
      241 ttcgccaggc agtccgtcag tttcatccgc aatctttcca acaattgcgt aagtttattc
      301 ctgtgggaaa tcaatgcggt aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt
      361 tgatgcagat gccagaattt aaagagattg cctgaagacc acgcttattt tttgacatcc
      421 cgctagcccc atctacgctc taatagtgga agcggaggga ctataaaatg aaaggtgatg
      481 ttaaaatcat aaattatctc aataaactat tgggaaatga gcttgtcgca atcaatcagt
      541 attttctcca tgcccggatg tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat
      601 accatgaatc catcgatgag atgaaacacg ctgataaata tatcgagcgt attttgtttt
      661 tagaaggaac ccccaactta caggatttag gcaaactggg gattggtgaa gatgtcgagg
      721 agatgctgca atccgacctg cgattagaac aggaaggcgc gaaggatctg cgtgaggcta
      781 tcgcatatgc cgacagcgtt catgactatg ttagccgcga tatgatgatc gagatccttg
      841 ctgatgaaga aggtcatatc gactggctcg aaactgaact ggatctcatc ggtaaaattg
      901 gcctacaaaa ttacctgcaa tcacaaatta aagttaaaga ttaacggcct atcagcaaac
      961 tatcccagcc tataacgaaa cccgcagccg ccagaaatgg cccaaaaggc
//
LOCUS       DAZPEB010000398         1009 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZPEB010000398 DAZPEB010000000
VERSION     DAZPEB010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.404"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..672
                     /locus_tag="ACHURJ_004330"
     CDS             <1..672
                     /locus_tag="ACHURJ_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA
                     acyltransferase"
                     /protein_id="HFR1283000.1"
                     /translation="LQSIVMMAELAIRGPEKFQKRVFWKGLEILEEIRYNNRNVIFLV
                     PHGWSVDIPAMLLAAQGEKMAAMFHQQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFI
                     QSVRQGYWGYYLPDQDHGPEYSEFADFFATYKATLPIIGRLMNISQAMIIPLFPVYDE
                     KKHFLTIEVRPPMDACIASADNKMIARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNE
                     ADPYP"
     gene            complement(826..>1009)
                     /locus_tag="ACHURJ_004331"
     CDS             complement(826..>1009)
                     /locus_tag="ACHURJ_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HFR1283001.1"
                     /translation="IYQPVYSPWVNHAERLWQALHDTITRNHQCRSMWQLLKKVRHFM
                     ETVSPFPGGKHGLAKV"
BASE COUNT          278 a          209 c          241 g          281 t
ORIGIN      
        1 ttgcaatcca tagtgatgat ggcagaactg gcaattcgtg gtccggaaaa gttccagaaa
       61 cgagtgttct ggaaaggcct cgaaattctt gaggaaattc gatacaataa cagaaatgtg
      121 atttttctgg ttccccatgg ctggagcgtg gatattcctg caatgttgct ggcagctcag
      181 ggggaaaaaa tggccgccat gtttcatcag caacgaaatc cagtgattga ttatgtctgg
      241 aattctgtac ggcgtaaatt cggggggcgc ttacattccc gggaggatgg gataaaacca
      301 tttattcagt cagtacgcca gggatactgg gggtattacc ttccagatca ggatcatggt
      361 cctgaataca gtgaatttgc tgattttttt gcgacctata aagcgacatt accaattatt
      421 ggacgtctga tgaacatcag tcaggctatg attataccgc ttttcccggt ttatgatgaa
      481 aaaaaacatt tcctgactat tgaagttcgg ccaccaatgg atgcatgcat tgccagcgcg
      541 gacaataaaa tgattgcccg acaaatgaac aaaacagtgg aaattttggt ggggtcacat
      601 ccggaacagt atatctgggt tttaaaattg ttaaaaacgc gcaaatcaaa cgaagcggac
      661 ccgtaccctt gaaattataa agtacaggta tttccactag ttgtttctta caggttacca
      721 atcgaaacac atcccccttc cgtgtgttgc ccgtttggca cggaggactg tgtagatgca
      781 gttcgttaaa tttaatttat gcctaaatag ctgcgcttaa taccgctaca cttttgccag
      841 tccgtgtttg cctccgggga atgggctgac ggtttccata aaatggcgaa cttttttcaa
      901 cagttgccac attgagcggc actgatgatt acgcgttatt gtgtcgtgaa gtgcctgcca
      961 tagccgttca gcatgattca cccatggcga gtaaaccggc tgataaatg
//
LOCUS       DAZPEB010000399         1006 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZPEB010000399 DAZPEB010000000
VERSION     DAZPEB010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.405"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..>1006
                     /locus_tag="ACHURJ_004332"
     CDS             <1..>1006
                     /locus_tag="ACHURJ_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFR1283002.1"
                     /translation="VEERQRGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETA
                     RLRYGYSSMTTPDTLFELDMDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPV
                     SLVYHRKHFRKGHNPLLVYGYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELG
                     QQWYEDGKFLKKKNTFNDYLDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPE
                     LFHGVIAQVPFVDVVTTMLDESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQA
                     YPHLLVTTGLHDSQVQYWEPAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYE
                     G"
BASE COUNT          244 a          223 c          266 g          273 t
ORIGIN      
        1 ggttgaagag cgtcagcgcg ggttaaccag tttgcgccaa attaaccgca agacccggga
       61 agtcattggt attgcctttg atgatccagc ctatgtgacc tggattgcct acaatccaga
      121 acctgaaacc gcgcgattgc gttatggtta ttcttccatg accacaccag acactttgtt
      181 tgaactggat atggataccg gtgagcgtcg tgtattaaaa caaacggaag ttcctggttt
      241 tgatgcggcg aattaccgca gtgaacacct gtggatagtc gcccgtgatg gcgtcgaagt
      301 tccggtttcg ttggtctacc atcgcaaaca ttttcgcaaa ggacacaacc cgttgctggt
      361 gtatggctat ggttcttacg gcgcaagtat tgatgccgat ttcagtttta gccgcttgag
      421 tttgttagat cgtggctttg tctacgccat tgtccatgtt cgcggcggtg gtgagctggg
      481 gcaacaatgg tacgaagacg gaaaatttct gaagaagaaa aatacgttta atgattatct
      541 tgatgcctgc gatgcattgt taaaactggg ctatggctct ccttcgcttt gttatgcgat
      601 gggcgggagt gcggggggca tgttgatggg cgttgcaatt aatcaacgcc cggaattatt
      661 ccacggcgtt atcgcccagg taccgtttgt tgatgttgta acaacgatgc ttgatgaatc
      721 aattcctctt accactggtg agtttgaaga gtggggtaac ccgcaggatc cgcaatatta
      781 cgagtacatg aaaagctaca gcccgtatga caacgtcacc gcacaggctt atccgcattt
      841 actggtaacg accggtttac acgattctca ggtgcaatat tgggaaccgg caaaatgggt
      901 cgctaaattg cgcgagctga aaaccgataa ccatctttta ttgctctgta ccgacatgga
      961 ctcaggccat ggcggtaaat ctggccgctt taaatcgtac gaaggc
//
LOCUS       DAZPEB010000400         1002 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZPEB010000400 DAZPEB010000000
VERSION     DAZPEB010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.406"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..721)
                     /locus_tag="ACHURJ_004333"
     CDS             complement(<1..721)
                     /locus_tag="ACHURJ_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin"
                     /protein_id="HFR1283003.1"
                     /translation="MTKISERWKHNGITEGYCNICGKYGLLTKDHVPPKCAITLGPVL
                     QKTVSEFFGIQEPVKPLNAKNGSYFRTICSHCNNKVLGGLDVAIENVTKSFKEQLSRY
                     MNGMNVYPFIRIPFDSISFTKAMIGHVLSATSVEDCKKEPVDSPFYTPLKDYVLGKNS
                     SFEETHDIYYWFYPHRMHISAQSVAFMNEGHVAFICALHFFPIGFIITMKNEGTYPAH
                     STKLELEDKFLTFNMTSINYEY"
BASE COUNT          315 a          198 c          169 g          320 t
ORIGIN      
        1 tatactcata atttatagag gtcatattaa atgtaaggaa tttatcttca agttctaatt
       61 ttgtagagtg ggcagggtat gttccttcat ttttcattgt gataataaaa ccgataggaa
      121 aaaaatgtaa agcacaaata aatgccacat gtccttcgtt cataaatgct acactttgag
      181 cggatatgtg cattctgtgt gggtaaaacc agtagtaaat atcatgagtt tcctcaaaac
      241 ttgaattttt acccaaaaca taatccttta aaggtgtata gaaggggcta tctacgggtt
      301 cttttttaca atcttcaact gaagttgctg atagcacgtg cccaatcata gccttagtga
      361 aagatatgct atcaaaaggt attctaatga atggatatac attcatacca ttcatatatc
      421 gacttaactg ttctttaaag gactttgtta cattttcaat tgcaacatcg agcccaccta
      481 ataccttatt gttacagtgg ctgcaaatgg ttctgaaata agagccattt ttagcattta
      541 atggtttaac tggttcctga ataccaaaaa attcgctaac tgttttctgc aaaacagggc
      601 ctaaggttat ggcgcacttt ggaggaacat gatcttttgt gagcaagcca tatttcccac
      661 aaatattgca ataaccttca gtaatgccat tgtgcttcca tcgttcacta attttcgtca
      721 tttttattcc ctcagtagca taagctatct atacgttttt taatcctatc aaagatacaa
      781 cttaacgtca ggaaaatgat agtgtttatc taagtaatac aattaacttc aacgcgtgac
      841 agtctgcttt tcgctcttag cggacattag cataggctat ttaccataac gcctcattac
      901 gcgtaccgct cagacggcct gagcgcgttt ctgacgtatc cccgttaaaa acagtaacaa
      961 accacccgaa aatgcacgcc agaaacgcta cgtaagaatc ta
//
LOCUS       DAZPEB010000401          999 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZPEB010000401 DAZPEB010000000
VERSION     DAZPEB010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..999
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.407"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            1..390
                     /gene="ipgA"
                     /locus_tag="ACHURJ_004334"
     CDS             1..390
                     /gene="ipgA"
                     /locus_tag="ACHURJ_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HFR1283004.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            406..>999
                     /gene="ipgB1"
                     /locus_tag="ACHURJ_004335"
     CDS             406..>999
                     /gene="ipgB1"
                     /locus_tag="ACHURJ_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HFR1283005.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSHNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTSYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVL"
BASE COUNT          372 a          176 c          151 g          300 t
ORIGIN      
        1 atgtgtcgca aactatatga taaactttat gaaataacag gtgcaaagct tgattttaac
       61 gataaaaatc aagcctttat attgctcgaa gaacaaatac ctgtttgtat aacagataat
      121 gatgaataca tctttttgac cggacttctt aatgaacatg aactttttac agaaaatatt
      181 attaatccag aacacatact catccttaac tactctttga gtagagatta tggttcttct
      241 atctgtcttc tccctgatac tcatcaatgt gttttgacca agaaacacta caaaaaatat
      301 ctttcacctg atgaactcat tgaatctttg tacgaatttt tattttgtat taaactaacc
      361 attgcaaaca tcacttcaga agtgaactaa catatagggg gtatcatgca aattctaaac
      421 aaaatacttc cacaggttga attcgcgatt cctcgcccat catttgattc gttaagtcac
      481 aataagcttg taaagaaaat attatctgtt tttaacttaa aacaacgctt tcctcagaag
      541 aattttggat gcccggtgaa tataaataaa atcagggaca gcgtcataga caaaataaaa
      601 gatagtaatt cggggaatca actattttgt tggatgagcc aagagcgaac ctcttatgtc
      661 tcttcaatga taaacagaag tatagatgaa atggccatac acaatggagt tgttttgacc
      721 tcagataaca aaaaaaacat atttgcagct attgaaaaaa aattcccgga tataaaactt
      781 gatgaaaaat cagcacaaac atctatcagt catacagcac taaacgagat tgcctcatct
      841 ggcctcagag caaagatttt aaaacgttat agcagtgaca tggatttgtt taacacacaa
      901 atgaaggatc ttacaaatct agtatccagt tccgtctacg ataaaatatt taatgaatca
      961 acaaaagttt tacaaataga aataagcgca gaggttcta
//
LOCUS       DAZPEB010000402          999 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZPEB010000402 DAZPEB010000000
VERSION     DAZPEB010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..999
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.408"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..116
                     /locus_tag="ACHURJ_004336"
     CDS             <1..116
                     /locus_tag="ACHURJ_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR1283006.1"
                     /translation="VSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            113..463
                     /gene="tnpB"
                     /locus_tag="ACHURJ_004337"
     CDS             113..463
                     /gene="tnpB"
                     /locus_tag="ACHURJ_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR1283007.1"
                     /translation="MISLPSGTRIWLVAGITDMRKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDMIKILWADADGLCLFTRRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            494..>999
                     /locus_tag="ACHURJ_004338"
     CDS             494..>999
                     /locus_tag="ACHURJ_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
                     /protein_id="HFR1283008.1"
                     /translation="MNQKYLIRIAELECQLRQKDQQLSLVEETEAFLRSALARAEEKI
                     EEDEREIEHLRAQIEKLRRMLFGTRSEKLRREVEQAEALLKQREQDSDRYSGREDDPQ
                     VPRQLRQSRHRRPLPEHLPREINRLEPEESCCPECGGELDYLGEVSAEQLELVSSALK
                     VIRTERVK"
BASE COUNT          254 a          255 c          284 g          206 t
ORIGIN      
        1 cagttagtgc cagctcctgc aaggtggagt tccgtcacgg taacatgacg ctggaaaatc
       61 cttcaccaga gctgctcaca gtgttgatcc gtgaactgac cgggagggga cgatgatctc
      121 actcccgtca ggcacccgca tctggctcgt tgctgggata accgatatgc gtaagtcttt
      181 caacgggctg ggtgaacagg tacagcatgt gctgaatgat aatcccttct ccggtcacct
      241 gttcatcttc cgtggccgac ggggtgacat gattaaaatc ctgtgggctg atgctgatgg
      301 tctgtgcctg ttcaccagac gcctggagga aggccagttt atctggcctg ctgtgcgtga
      361 cggcaaggta tccattaccc gctcgcaact ggcaatgctc ctcgataagc tggactggcg
      421 tcagccaaaa acatcccgcc ttaatgcact gacaatgttg taaaaatgtc atggccggat
      481 tataaaaacg gccatgaatc aaaaatacct cattcgcatt gcagaactgg aatgccagct
      541 ccgtcagaaa gaccagcaac tgagtctggt tgaagagacg gaggccttcc tgcgctctgc
      601 actggcccgc gccgaagaaa agatcgaaga agatgaacgg gaaatagaac atctgcgggc
      661 tcagatagaa aaactgcgcc ggatgctgtt cggtacccgt tctgaaaaac tgcgtcgtga
      721 agttgaacag gctgaggccc tgctgaaaca acgcgaacag gacagtgatc gttacagtgg
      781 gcgggaagac gatccgcagg ttccccgcca gttgcgacag tctcgtcatc gtcgcccgtt
      841 accggagcat ctgccccgcg aaataaatcg cctggagcca gaagaaagct gttgcccgga
      901 gtgtggcggt gagctggatt atctggggga agtcagcgca gaacaactgg aactggtgag
      961 cagcgctctg aaagtgatcc gcacagaacg ggtaaaaaa
//
LOCUS       DAZPEB010000403          974 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZPEB010000403 DAZPEB010000000
VERSION     DAZPEB010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..974
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.409"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..744
                     /locus_tag="ACHURJ_004339"
     CDS             <1..744
                     /locus_tag="ACHURJ_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFR1283009.1"
                     /translation="YTPARKIHLYHCDHRGLPLALISTEGATEWCAEYDEWGNLLNEE
                     NPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNFYQYPLNP
                     ISEIDPQGLNPLILGVVALMSCGLFSSGGVIQSGQQRRDQYGRKVNPEADKLTDEHKS
                     GVNPGGNCSPQNLSDLQNEKNRLCNQSRACAPQMLRKEILRRYEINLACASVRKQINN
                     QCFGGGDKAHMEEENKAYKTAADCSGLIK"
     gene            741..>974
                     /locus_tag="ACHURJ_004340"
     CDS             741..>974
                     /locus_tag="ACHURJ_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1283010.1"
                     /translation="MKKIIFSNESAVYDELMIHFPCQPLPHISNDIIGLDELDVVYNF
                     FQKKQWNEIANNLKIKDDSYALELGITFLPEKVF"
BASE COUNT          310 a          188 c          235 g          241 t
ORIGIN      
        1 tacacgccgg cgcgaaaaat ccacctgtac cactgcgacc atcgcggcct gccgctggcg
       61 cttatcagca cggaaggggc aacagaatgg tgcgcagaat acgatgagtg gggcaacctg
      121 ctgaatgaag agaacccgca ccagctgcag cagcttatcc gtttgccggg gcagcagtat
      181 gatgaggagt ccggcctgta ttacaaccgc caccgctatt atgacccgct gcaggggcga
      241 tatatcactc aggacccaat tgggctgaag gggggatgga atttttatca gtatccgttg
      301 aatccgatct cagagataga cccacaaggg ttaaatccgt taattcttgg tgttgtagca
      361 ctgatgagtt gcggtttgtt ttcatcaggt ggtgttatcc aaagtgggca acaacgcaga
      421 gatcaatatg gacgtaaagt taatccagaa gccgacaaat taacagatga acataagtca
      481 ggtgtaaatc ctggaggtaa ctgtagtccc cagaacctga gtgacttaca aaatgaaaaa
      541 aacagattat gtaaccagtc tagagcttgc gctcctcaga tgctgagaaa agaaatattg
      601 agacggtacg agataaattt ggcgtgcgct tcggtcagaa agcagattaa taatcagtgc
      661 tttggtggtg gagataaggc ccatatggaa gaagagaata aagcatataa aacagcagct
      721 gattgcagtg gcttaataaa atgaaaaaaa taattttctc taacgagagt gcagtctatg
      781 atgaacttat gattcatttt ccatgccagc cattacctca tatttcaaat gatattatag
      841 ggctggatga actggatgtt gtatataatt ttttccaaaa gaaacaatgg aatgaaatag
      901 ccaataattt aaaaataaag gatgactctt atgctttaga gttaggaatt acattcctcc
      961 ctgagaaagt attc
//
LOCUS       DAZPEB010000404          969 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZPEB010000404 DAZPEB010000000
VERSION     DAZPEB010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..969
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.410"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(1..304)
                     /locus_tag="ACHURJ_004341"
                     /pseudo
     CDS             complement(1..304)
                     /locus_tag="ACHURJ_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            288..546
                     /locus_tag="ACHURJ_004342"
                     /pseudo
     CDS             288..546
                     /locus_tag="ACHURJ_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(655..>969)
                     /locus_tag="ACHURJ_004343"
     CDS             complement(655..>969)
                     /locus_tag="ACHURJ_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1283011.1"
                     /translation="LNSSLYITPGESGFVSFVNREGKICHTAYVKSSDNSMAYYHANY
                     SSIDKYITDMCGLICMRHIESTCIIFYMLDEKVLSAIAEFMNEKGWRAAFCSAKNLYK
                     CV"
BASE COUNT          271 a          243 c          179 g          276 t
ORIGIN      
        1 cctcgctcca tggtctgctc cagatatcct ttgccagttc tttcagcgcc cagcacaggc
       61 tcgtcagctt catctgcgca cgcagccata tcagcttttc ctgcctggat tcggtcatcc
      121 atttatcgct gtgctgccac aggaagcgag ttccctttgc ctgacgacga ctttcaacag
      181 gaagatgcgg atgttcattc tggcgggttt tatccaccac ctcacccggt tgtttcgcca
      241 catgaaagcg atcgaaggct attttctcaa ccgcattggg taagtggatg cgcgctgctc
      301 ttagtttgct gagccagttt agcattgcct tctacatgac cgatgcctga gccggtatac
      361 cgaactttat tgtcctcaac cagtcatgtt ataagcaaga aatgcactca gcggatagag
      421 cgacataatc tgaacctgcg gacacatctt aagcggctta cccgcaaaac catatgcttc
      481 tcaaagtctg atgatatgca ctataagatc atcggctggt atctgacaat caaccaccac
      541 cactaaatct gcgtcacgac ctgcaactga gtctggttga acaggctgag gccctgctga
      601 agcaacgcga acctctcgac tcttgttgga ttttaccaaa tagtttttct aaaattaaac
      661 acatttatat aagttctttg ctgaacaaaa cgcagcccgc catccttttt cattcataaa
      721 ttcagcaatg gcagataaaa ctttttcatc aagcatataa aaaataatgc aagtagactc
      781 aatatgcctc atacaaataa ggccgcacat atccgtaata tatttgtcaa tgctgctata
      841 atttgcatgg taatatgcca tgctattatc tgatgatttt acatatgcag tatgacatat
      901 ttttccttca cgattaacaa aactgacaaa accactttca ccaggagtaa tatataaaga
      961 tgaatttaa
//
LOCUS       DAZPEB010000405          947 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZPEB010000405 DAZPEB010000000
VERSION     DAZPEB010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..947
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.411"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            142..947
                     /locus_tag="ACHURJ_004344"
                     /pseudo
     CDS             142..947
                     /locus_tag="ACHURJ_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
BASE COUNT          305 a          136 c          168 g          338 t
ORIGIN      
        1 ttttatagta cttcacttct tcttcactaa gaatgtccat tatctcaatg ccttatcaga
       61 gattcttttc ctttcgccgg tagtgtctgg acgctcaggc tacttttcca ggttatttta
      121 tttctgtatt acagaggttt tatgataggt catatcacaa attctggtgg caataactct
      181 ttgatgaaac atgatgtggt tcaaggaaat aatatagtag atcttgattt actacgtaat
      241 ttcaatgggg tgacaggttt aaacagagat aactttattt atatcagtga tatattttta
      301 aatataaaac aacggaacga aaaaatcatg caataaatat gttacgtgaa gtctcaatta
      361 gtaatgatac tataagtgta aaattctaca gaaatgaaaa aatagaatgc gcttgtaatt
      421 ttctgatgga taaagattcg caggggtata tcgacctgtc tgattttgat gtaacaaatt
      481 gtcattttaa aggtgacgtt atttctaagg tgtctttttt atcatcaaat ctacaacatg
      541 taactttcga atgtaaagaa attgagaatt gcaattttac taaggcaaca gttgataatg
      601 tcatatttaa atgtcgacgt ttacacaatg tgatttttct caaaacgagt ggcgaatgtg
      661 ttgattttag ccaagatatt cttgatacag ttgacttctc gcagagtcaa cttggtcata
      721 gtaattttcg cgaatgtcag attagaaatt caaacttcga taattgttat ctttacgctt
      781 cgcacttcac cagagcagag tttctgtctg ccaaagaaat atcatttatt aaatcgaatt
      841 tgacagctgt tatgtttgat catgtgcgaa tgtcgacagg gaattttaaa gattgcatta
      901 cagaacaatt ggaattaact attgattatt cagatatatt tgggaat
//
LOCUS       DAZPEB010000406          941 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZPEB010000406 DAZPEB010000000
VERSION     DAZPEB010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..941
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.412"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..565
                     /gene="envY"
                     /locus_tag="ACHURJ_004345"
     CDS             <1..565
                     /gene="envY"
                     /locus_tag="ACHURJ_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFR1283012.1"
                     /translation="LLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAANHSKQQPS
                     KPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQHYWNLRIV
                     ASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQLCGYSSTS
                     YFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(611..687)
                     /locus_tag="ACHURJ_004346"
     tRNA            complement(611..687)
                     /locus_tag="ACHURJ_004346"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(651..653),aa:Arg,seq:tct)
BASE COUNT          259 a          227 c          217 g          238 t
ORIGIN      
        1 tctgttgttt ttaaataaga acttaacctg tgtaaagccc tggtcgcggc tggcaacccc
       61 ggttatcgct tgtcatagcc gtacaccgga agtgttccgg ctagccgcca accacagcaa
      121 gcagcaaccc agcaaaccct gcgaagcgga gttgacgcgc gcattacttt ttaccgtatt
      181 gtctaacttt cttgagcaat cgcggtttat tgccctactg atgtatatct tacgcagcag
      241 cgtccgcgac agcgtctgcc gcattattca aagcgatatt cagcattact ggaatctgcg
      301 aattgtcgcc agttcgctat gtttaagccc cagcctgctc aaaaagaaat taaaaaacga
      361 aaataccagc tatagccaga ttgtcacaga gtgtcgtatg cgttacgccg tacagatgtt
      421 attgatggat aacaaaaata tcactcaggt ggcgcaatta tgtggctata gcagcacgtc
      481 gtactttatc tctgttttta aggcgtttta cggcctgaca ccgttgaatt atctcgccaa
      541 acagcgacaa aaagtgatgt ggtgaagggc aaagcggaaa cggataagac gggcataaat
      601 gaagaagaaa tggcgcgccc tgcaggattc gaacctgcgg cccacgactt agaaggtcgt
      661 tgctctatcc aactgagcta agggcgcgtt gataccgcaa tgcggtgtaa tcgcgtgaat
      721 tatacggtca acccttgctg agtcaatggc ttttgatctg gttgctgaac aagtgaacga
      781 ccgcgtctga ttttctgatt tatttcgcta tagcggcaaa caaacgcaca ccgctgcgcg
      841 tctgaatcaa gaaaacccgt attttcatgt atcaaagtac aatttaccgc cctaacggaa
      901 aattgtccgc tcctatgaga ctggtaacta tgaaaccaac g
//
LOCUS       DAZPEB010000407          921 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZPEB010000407 DAZPEB010000000
VERSION     DAZPEB010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..921
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.413"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..>921)
                     /locus_tag="ACHURJ_004347"
     CDS             complement(<1..>921)
                     /locus_tag="ACHURJ_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HFR1283013.1"
                     /translation="RFSYGITGGNVFFSCLVFVFFYFAILMTVSADMPLVGCVLTLSL
                     LVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMAIGMGTVFFIST
                     KMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFKVKLDT
                     AYCIKYAILAFLALLPFLAVAGYIIFEQILNAYDSSVYANDDIENLQQFMEMQRKMII
                     AQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCALVVISG
                     ITGGLAYPLLKIWMIDWQAKNTYLLGDLDDL"
BASE COUNT          354 a          207 c          141 g          219 t
ORIGIN      
        1 aaggtcatcc aaatcgccca gcaaatacgt attttttgcc tgccagtcaa tcatccatat
       61 tttcagcagt ggataagcca gaccgcccgt aatcccggat atcactacca acgcacacat
      121 gcgataaagc ataccgtggt acgttaaagt tgagcgaaaa cgaatacgcc catcattcag
      181 tgacaggtta ctcataaaat gatttcgcaa cgacaccgtt aaataactgg tgctaacagc
      241 aatcccaaaa taatagatta actgcgcgat tatcatttta cgttgcattt ccataaattg
      301 ctgtaaattc tcaatatcat catttgcata cacagaacta tcatacgcat ttaatatttg
      361 ttcgaagata atataaccag caacagcgag aaaaggtaat aaagctaaaa atgcgaggat
      421 ggcatatttt atacaatacg cagtatctaa tttcaccttg aaacgatgta taccgaaact
      481 ggtattgctc cagagaaaac tcattaccag gctatataaa gtaccattaa aaataccaat
      541 ggaaacaata ccaactattg ccatcagaac cacagatata ataacactac ttgaactatt
      601 ggcatgtagc atctttgtcg agataaagaa aacagtcccc atcccaatgg ccattaaaat
      661 cggcaagaaa aaggttaccc accagaaccc tttcatagag caattaaaac taaatcttac
      721 gccgttgaga ctagtcatca aggcctgata acgcagtcct tttgctgcca taaatataag
      781 caaaaccaac agtgacaaag tcaaaacaca gccaacaagc ggcatatctg ctgacactgt
      841 cattaagatt gcgaaataga aaaaaacaaa aacaagacaa ctaaaaaaaa cattcccacc
      901 agtaattcca taagaaaatc g
//
LOCUS       DAZPEB010000408          919 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZPEB010000408 DAZPEB010000000
VERSION     DAZPEB010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..919
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.414"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..849
                     /locus_tag="ACHURJ_004348"
     CDS             <1..849
                     /locus_tag="ACHURJ_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1283014.1"
                     /translation="AEQAKNDIDAALTGTLKMANHLSEIAAAGEKAQQKSRDNLGLKS
                     AATMEAQSDIYDRTKGRLAIPGAFGFGCAFLPEDVIRFDTKSDFLAWVRNALPGEYSV
                     AGPYDIIIPDTRFEGVLSIRWTDARPETTEPRYRAKSLTFYGINGPIYHTRYCYWPIS
                     RLTGWVKINITTEDIIYRIVASSVRNRWGDPDIGGLIIAAYQGEADGDKVIRLVRGQS
                     YRGSRLGPVGISVPSTPTGTYIASPQFFITGCSEHSLPGSYCALSGGPDVHVSGAMPG
                     LFIRTS"
     gene            800..>919
                     /locus_tag="ACHURJ_004349"
     CDS             800..>919
                     /locus_tag="ACHURJ_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1283015.1"
                     /translation="MYMSLAQCPGFLFAHREECTVEIKKIINPRYTESGAVDCD"
BASE COUNT          239 a          231 c          246 g          203 t
ORIGIN      
        1 gcagaacagg ccaaaaatga cattgatgct gcgctgaccg gcactctgaa aatggctaac
       61 catctgtcag aaatcgcagc agcaggcgaa aaggcacaac agaagtcccg ggataatctg
      121 gggctgaaaa gtgcggccac gatggaagca cagagcgaca tttacgaccg gacaaaaggc
      181 cgtctggcga tacccggcgc attcggcttt gggtgtgctt ttctgcctga agatgttatc
      241 cgttttgaca ctaagagtga tttcctggcc tgggtaagga atgcgctgcc aggtgaatat
      301 tccgttgctg gcccctacga catcatcata cccgacacac ggtttgaagg ggtgctcagc
      361 atccggtgga ctgatgcacg ccctgagaca acagaaccgc ggtacagagc caaatccctt
      421 actttttacg gcattaacgg ccccatttat cacacccgct actgctactg gcccatatcc
      481 agactgactg gctgggtgaa aataaatata accacagaag atattattta cagaatcgtg
      541 gcgagctctg tccgcaacag atggggagac cctgacattg gcgggctgat tattgctgcg
      601 taccagggag aagctgacgg tgataaagtc atcagacttg tcagggggca gtcatacaga
      661 ggctcacgac tgggaccggt ggggatttca gtgcccagta ctcccaccgg aacgtatata
      721 gcatccccac aatttttcat tacgggatgt tcagagcatt cattaccggg gtcatattgc
      781 gccctgtccg gggggccgga tgtacatgtc tctggcgcaa tgcccgggct ttttattcgc
      841 acatcgtgag gaatgcaccg tggaaattaa aaaaatcatt aatccccgtt ataccgaaag
      901 tggcgcagta gactgtgac
//
LOCUS       DAZPEB010000409          919 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZPEB010000409 DAZPEB010000000
VERSION     DAZPEB010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..919
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.415"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            1..625
                     /locus_tag="ACHURJ_004350"
                     /pseudo
     CDS             1..625
                     /locus_tag="ACHURJ_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
     gene            676..>919
                     /gene="chbA"
                     /locus_tag="ACHURJ_004351"
     CDS             676..>919
                     /gene="chbA"
                     /locus_tag="ACHURJ_004351"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFR1283016.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKM"
BASE COUNT          222 a          224 c          254 g          219 t
ORIGIN      
        1 ggttcttcgg tattcatggc gcgctggcgc tgaccgcact ggacaacggc attatgacgc
       61 tgtgggcgct ggaaaatatc gcgacctatc agcaatatgg ttccgtcgaa gcggcgctgg
      121 cagccggtaa gaccttccat atctgggcca agccgatgct ggactccttt attttccttg
      181 gggcagtggt gcgactttag gcctgatcct ggctatcttt atcgcctctc gccgtgctga
      241 ttatcgtcag gtggcaaaac tggcgctgcc gtccggcatc ttccagatta acgaaccgat
      301 tctgtttggt ctgccaatta tcatgaaccc ggtgatgttt atcccgtttg tactggtaca
      361 accgattctg gcggcaatca ccctcgcagc gtactacatg ggcattattc ctccggtgac
      421 caatattgca ccgtggacca tgccaaccgg tctgggagcc ttctttaaca ccaacggtag
      481 cgtcgctgca ttgctggtcg cactcttcaa ccttggtatc gcaacgttaa tttatctgcc
      541 ctttgttgtg gtggctaaca aagcgcaaaa cgcgattgat aaagaagaga gcgaagaaga
      601 tatcgctaac gccctgaaat tttaatcgat gccggtacgg ttaatcgtgc cggtaagaag
      661 agagaggaac gatgtatgat ggatctcgat aacattcccg atacgcaaac agaagctgaa
      721 gagctggaag aagtggtgat ggggcttatc atcaactccg gacaagcgcg cagcctggcg
      781 tatgcggcac tgaaacaggc gaagcagggc gattttgccg cagcaaaagc catgatggat
      841 cagtcacgga tggcattgaa tgaagcgcat ctggtacaga cgaaactgat tgaaggcgat
      901 gcgggcgaag gtaagatga
//
LOCUS       DAZPEB010000410          914 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZPEB010000410 DAZPEB010000000
VERSION     DAZPEB010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..914
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.416"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..>914
                     /gene="tuf"
                     /locus_tag="ACHURJ_004352"
     CDS             <1..>914
                     /gene="tuf"
                     /locus_tag="ACHURJ_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFR1283017.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGII
                     KVGEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAK
                     PGTIKPHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMP
                     GDNIKMVVTLIHPIAMDDGLRFAIREGGR"
BASE COUNT          212 a          230 c          264 g          208 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aactggctgg cttcctggat
      361 tcttacattc cggaaccaga gcgtgcgatt gacaagccgt tcctgctgcc gatcgaagac
      421 gtattctcca tctccggtcg tggtaccgtt gttaccggtc gtgtagaacg cggtatcatc
      481 aaagttggtg aagaagttga aatcgttggt atcaaagaga ctcagaagtc tacctgtact
      541 ggcgttgaaa tgttccgcaa actgctggac gaaggccgtg ctggtgagaa cgtaggtgtt
      601 ctgctgcgtg gtatcaaacg tgaagaaatc gaacgtggtc aggtactggc taagccgggc
      661 accatcaagc cgcacaccaa gttcgaatct gaagtgtaca ttctgtccaa agatgaaggc
      721 ggccgtcata ctccgttctt caaaggctac cgtccgcagt tctacttccg tactactgac
      781 gtgactggta ccatcgaact gccggaaggc gtagagatgg taatgccggg cgacaacatc
      841 aaaatggttg ttaccctgat ccacccgatc gcgatggacg acggtctgcg tttcgcaatc
      901 cgtgaaggcg gccg
//
LOCUS       DAZPEB010000411          914 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZPEB010000411 DAZPEB010000000
VERSION     DAZPEB010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..914
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.417"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..186)
                     /locus_tag="ACHURJ_004353"
     CDS             complement(<1..186)
                     /locus_tag="ACHURJ_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0001907 - symbiont-mediated killing of host
                     cell [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFR1283018.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIRE"
     gene            complement(479..553)
                     /locus_tag="ACHURJ_004354"
     tRNA            complement(479..553)
                     /locus_tag="ACHURJ_004354"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(518..520),aa:Gly,seq:tcc)
     gene            complement(557..632)
                     /locus_tag="ACHURJ_004355"
     tRNA            complement(557..632)
                     /locus_tag="ACHURJ_004355"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(597..599),aa:Thr,seq:tgt)
     gene            complement(635..711)
                     /locus_tag="ACHURJ_004356"
     tRNA            complement(635..711)
                     /locus_tag="ACHURJ_004356"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(675..677),aa:Arg,seq:tct)
     gene            complement(713..789)
                     /locus_tag="ACHURJ_004357"
     tRNA            complement(713..789)
                     /locus_tag="ACHURJ_004357"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(753..755),aa:Ile,seq:cat)
BASE COUNT          266 a          234 c          212 g          202 t
ORIGIN      
        1 ttctcttatt ttgaaataca gattcgtcag ataagtcaga agccccagaa gcagacttcc
       61 cagcacacca atcgcagccc actgtgatgg actgacctga tccaaccact gcaaaaacca
      121 gtagccggca ctgccggcgg aggtgccgta ggcaatgccc gttgaaattt tgtccatgga
      181 tttcatagcc tcacctccgc aaataacgga tggcgtagtt ttacactgag aaatgaaagg
      241 gatttgaaaa gaaaaaaacg caaaagcggg cgaaacgata tatacagtaa ggaaagcact
      301 ctatccaaca aaccacccac agttaatcgg aataaaagca gagtgcttat gaatgatcgc
      361 acgcccgaag gttagtattt ctgcacagca attttgcaaa aaaaggcgat cattcataac
      421 ttaaacgtct ttcagtcact ccgggatttc ccatcatcgc agactgaaag actctgactg
      481 gagcgggcag cgggaatcga acccgcatca tcagcttgga aggctgaggt aatagccatt
      541 atacgatgcc cgcatatggt gccgactacc ggaatcgaac tggtgaccta ctgattacaa
      601 gtcagttgct ctgcctgctg agctaagtcg gcgctggccc accaccgagg actcgaacct
      661 cgcaccgtca acttagaagg ttgatgctct atccggatga gctagtggtg gttggtggcc
      721 cttgctggac ttgaaccagc gacctggcga ttatgagtcg ctcgctctga ccaactgagc
      781 taaagggccg gaggcagaat aataaccata tgtcatcaca tctgcaaact catctgacca
      841 ccagcacgtt taacgtcctg tgccgttttt caggtataaa aaaacccgca taaagcgggc
      901 tctttcaaat gtcc
//
LOCUS       DAZPEB010000412          909 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZPEB010000412 DAZPEB010000000
VERSION     DAZPEB010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..909
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.418"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..237
                     /gene="ipgE"
                     /locus_tag="ACHURJ_004358"
     CDS             <1..237
                     /gene="ipgE"
                     /locus_tag="ACHURJ_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HFR1283019.1"
                     /translation="LLCPFCALPENINDLIYALSLNYSEKICLATDDEGGSLIARLDL
                     TGINEFEDIYVNTEYYISRVRWLKDEFARRMKGY"
     gene            237..695
                     /gene="ipgF"
                     /locus_tag="ACHURJ_004359"
     CDS             237..695
                     /gene="ipgF"
                     /locus_tag="ACHURJ_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HFR1283020.1"
                     /translation="MSRFVFIFLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKFAVNVNNNGSKDYGIMQINDFHSKRLREMGYTEEMLISHPCLSVHYAAKLLNEFM
                     LMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYKRYLRIAAESKQNNRRI"
     gene            704..>909
                     /locus_tag="ACHURJ_004360"
     CDS             704..>909
                     /locus_tag="ACHURJ_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HFR1283021.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIY"
BASE COUNT          307 a          116 c          207 g          279 t
ORIGIN      
        1 ttattatgcc ctttttgtgc attgcctgaa aatataaatg atcttatata tgcgttgagc
       61 ctaaattact cagaaaagat atgtttagct actgatgatg aaggtggaag tctaattgcg
      121 cgattagatc ttaccgggat aaatgaattc gaggatatat atgttaatac agaatattat
      181 atttcgcgtg tgcgttggct gaaggatgaa tttgcgcgaa gaatgaaggg gtattaatgt
      241 cccgtttcgt atttattttt ctgtgtttta tcccatattt agggagagct gattgttggg
      301 ataaggctgg tgaaaggtac aacattccat cgagtttgtt aaaagcgatt gcggaaaaag
      361 aatccggatt taataaattt gcagtcaatg ttaataacaa tggaagtaaa gattatggta
      421 taatgcaaat aaatgacttt cattctaaaa gacttagaga aatgggatat actgaggaaa
      481 tgctaattag tcatccatgt ctttctgtac attatgcagc aaagttatta aatgagttta
      541 tgctgatgta tggaagaggg tgggaggctg ttggtgccta taatgcagga acatctccga
      601 aaaagaaaaa agaaagatta aagtacgcag aggatatcta taaacgctat ttgcggatag
      661 cagcagagag caagcagaat aatcgaagga tataagagga tttatgtctg aggcaaagaa
      721 ctcaaatctt gcaccattca ggttattagt gaaactcaca aacggtgtcg gggatgagtt
      781 tccgttatat tatgggaata acttaattgt tctcggacgg acgatagaaa cacttgagtt
      841 tggaaacgat aatttcccag aaaatattat tccagtaaca gatagtaaat cggatggtat
      901 aatttattt
//
LOCUS       DAZPEB010000413          896 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZPEB010000413 DAZPEB010000000
VERSION     DAZPEB010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..896
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.419"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..>896
                     /locus_tag="ACHURJ_004361"
     CDS             <1..>896
                     /locus_tag="ACHURJ_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFR1283022.1"
                     /translation="EHADGSVTQSQFDAVGRLRTQTDAAGRTTEYSPDVVTGLITRIT
                     TPDGRASAFYYNHHSQLTSATGPDGLEIRREYDEWGRLIQETAPDGDIIRYRYDNPHS
                     DLPCATEDATGSRKTMTWSRYGQLLSFTDCSGYVTRYDHDRFGQMTAVHREEGLSQYR
                     AYDSRGQLIAVKDTQGHETRYEYNAAGDLTAVIAPDGSRNGTQYDAWGKAIRTTQGEL
                     TRSMEYDAAGRVIRLTSENGSHTTFRYDVLDRLIQETGFDGRTQRYHHDLTGKLIRSE
                     DEGLVTHWHYDEADRLTHRTVNG"
BASE COUNT          221 a          267 c          267 g          141 t
ORIGIN      
        1 gaacacgcgg acggcagcgt cacgcagagt cagtttgacg ccgtgggcag gctcaggaca
       61 cagacggatg ccgcaggccg gacaacagaa tacagcccgg atgtggtgac gggcctcatc
      121 acgcgcatca ccacgccgga tggcagggca tcggcgtttt actataacca ccacagccag
      181 ttaacgtcag ccaccgggcc tgacgggctg gaaatacgcc gggaatatga tgaatggggc
      241 cgtctgattc aggaaactgc ccctgacggc gatatcatcc gctaccgtta tgataatcca
      301 cacagtgact taccctgcgc aacggaagat gccaccggta gccggaaaac catgacgtgg
      361 agccgttacg gtcagttgct gagcttcacc gactgttccg gttatgtaac ccgttatgac
      421 catgaccgct tcgggcagat gacggcggtg caccgcgagg aagggctgag tcagtaccgc
      481 gcatacgaca gccgtggaca gttaattgcc gtgaaagaca cgcagggcca tgaaacgcgg
      541 tatgaataca acgccgccgg tgacctgacc gccgtcattg ccccggacgg cagcagaaac
      601 gggacacagt acgatgcgtg gggaaaggcc atccgtacca cgcagggcga gctgacgcgc
      661 agtatggaat acgatgctgc aggacgggtc atccgcctga ccagtgaaaa cggcagccac
      721 accaccttcc gttacgatgt actcgaccgg ctgatacagg aaaccggctt tgacggccgc
      781 acacagcgtt atcaccacga cctgaccggc aaacttatcc gcagcgagga tgaggggctg
      841 gtcacccact ggcactatga cgaagcagac cgcctcacgc accgcaccgt gaatgg
//
LOCUS       DAZPEB010000414          892 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZPEB010000414 DAZPEB010000000
VERSION     DAZPEB010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..892
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.420"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..>892
                     /locus_tag="ACHURJ_004362"
     CDS             <1..>892
                     /locus_tag="ACHURJ_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HFR1283023.1"
                     /translation="YYIQLRGAFLSAIHHQIIENQLDTETITISYEFILCQHLIANTE
                     FSYLALPENYNRLFLPNSKNQTNNRFKTLNSKAIGRLLAAGGVYNGNIEGFRDTAEKL
                     GGDAIKGYDQILNEKTAGIAIATASILLTKRSNVDTYTEINSYLGKLRGQQKLLDGID
                     IIEIIYIKRPSKDLANLRKEFNKTVRKNFLIKLAKTSEASGRFNAEDLLRMRKGNVPL
                     NYNVHHKLSLDDGGTNDFENLVLIENEPYHKVFTNMQSRIAKGILVGESKITPWAIPS
                     GSIYPPMKNVMNASHTIKHGA"
BASE COUNT          343 a          162 c          146 g          241 t
ORIGIN      
        1 attattatat acaactaaga ggcgcttttt tatcggctat tcatcaccag atcattgaaa
       61 accaactgga tacagaaaca ataactatta gttatgaatt tatcctctgt caacatctta
      121 tcgcaaatac cgagttcagc tatttggcac tccctgaaaa ttataaccga ttgtttttac
      181 caaattcaaa aaaccaaaca aataatcgtt tcaaaacgtt aaacagcaaa gctattggca
      241 ggctacttgc tgctggtggc gtatacaatg ggaacattga aggattcaga gatactgcgg
      301 aaaaactggg tggagatgca ataaaaggct atgatcaaat actaaatgaa aaaacagcgg
      361 gcatagcgat agcaacagca tctattcttt taacaaagcg ttctaatgtt gatacatata
      421 cagaaataaa tagttactta ggcaaactta gaggtcaaca aaaacttctt gatggtatag
      481 acataataga aataatatac attaagagac cttcaaaaga cttagctaac ttacgaaagg
      541 agtttaataa aactgtaaga aaaaattttc ttatcaaact tgcaaaaacc tccgaagcat
      601 ctggaagatt caacgccgaa gaccttttaa gaatgagaaa gggcaatgtt cctctaaatt
      661 ataatgttca ccataaacta tctctagatg atggtggtac taatgatttc gaaaatttag
      721 tattaatcga aaacgaacca tatcataaag tttttactaa catgcaatca cgaatagcta
      781 agggaatatt agtaggtgaa agcaaaatca ctccctgggc cattccatct ggctcaattt
      841 atcctcccat gaaaaatgta atgaacgcat cccacacaat taaacacgga gc
//
LOCUS       DAZPEB010000415          880 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZPEB010000415 DAZPEB010000000
VERSION     DAZPEB010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..880
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.421"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            22..489
                     /gene="ipgC"
                     /locus_tag="ACHURJ_004363"
     CDS             22..489
                     /gene="ipgC"
                     /locus_tag="ACHURJ_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HFR1283024.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            495..>880
                     /locus_tag="ACHURJ_004364"
     CDS             495..>880
                     /locus_tag="ACHURJ_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasin"
                     /protein_id="HFR1283025.1"
                     /translation="MHNVSTTTTGLPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LAANKNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLS"
BASE COUNT          322 a          174 c          136 g          248 t
ORIGIN      
        1 tgcagacaaa aaggagacct tatgtcttta aatatcactg aaaatgaaag catctctact
       61 gcagtaattg atgcaattaa ctctggcgct acactgaaag atattaatgc aattcctgat
      121 gatatgatgg atgacattta ttcatatgct tatgactttt acaacaaagg aagaatagag
      181 gaagctgaag ttttcttcag atttttatgt atatacgact tttacaatgt agactacatt
      241 atgggactcg cagctattta tcagataaaa gaacagttcc aacaagcagc agacctttat
      301 gctgtcgctt ttgcattagg aaaaaatgac tatacaccgg tattccatac tggacaatgc
      361 cagcttcggt tgaaagcccc cttgaaagcc aaagagtgct tcgaactcgt aattcaacac
      421 agcaatgatg aaaaattaaa aataaaagca caatcatact tagacgcaat tcaggatatc
      481 aaggagtaat tattatgcat aatgtaagca ccacaaccac tggtttgcct cttgccaaaa
      541 tattggcttc cactgagctt ggagacaata ctatccaggc tgcaaatgat gcagctaaca
      601 aattattttc tcttacaatt gctgatcttg ctgctaacaa aaatattaat acaactaatg
      661 cacactcaac ttcgaatata ttaatccctg aacttaaagc accaaagtca ttaaatgcaa
      721 gttcccaact aacgctttta attggaaacc ttattcaaat acttggtgaa aaatctttaa
      781 ctgcattaac aaataaaatt actgcctgga agtcccaaca acaggcaaga cagcaaaaaa
      841 acctagaatt ctccgataaa attaatactc ttctatctga
//
LOCUS       DAZPEB010000416          879 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZPEB010000416 DAZPEB010000000
VERSION     DAZPEB010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..879
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.422"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..838)
                     /locus_tag="ACHURJ_004365"
     CDS             complement(<1..838)
                     /locus_tag="ACHURJ_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005006233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1283026.1"
                     /translation="MGITRSQWSILTEWFRNNQSLLNASLRSRYNRILRTYITVWEQY
                     QESINFRFCNTENSDLFELFCKTQLQELPSMIIDATQTRDPSTIDTAVDLINRWQHSM
                     NIDSHSVEKYSYKGQLFNPGFFISKKLNFNSDREWFNIAIINALTDMRICTCLYLTSR
                     INTSDKLMTHYIKLILEGKLIDQTGGYETPTEEIDNASQLIKILIRICLWTWSENMEH
                     NGWMNSLARRLRDYDKTDMVMGRVYSNVFDCGFIDMEQSWVQLLLIFSKKNDSVSKEI
                     KEA"
BASE COUNT          260 a          153 c          154 g          312 t
ORIGIN      
        1 ttgcttcttt tatttctttt gatacgctat cgttcttttt agaaaaaatt aataatagct
       61 gaacccatga ttgttccata tcaataaatc cacagtcaaa aacattactg taaactctgc
      121 ccatcaccat gtctgtttta tcatagtcac gtaaacgcct tgctaaacta ttcatccacc
      181 cattgtgctc catattttca gaccatgtcc acaggcagat tctaattaat atcttgatta
      241 gttggcttgc attatcaatt tcttcagtgg gagtttcata tccaccggtt tggtcaataa
      301 gtttaccctc taaaataagt ttaatgtaat gtgtcattag tttgtcagaa gtattgattc
      361 ttgatgtcag ataaagacat gtgcatatac gcatgtcagt caacgcattt atgatggcaa
      421 tattgaacca ctctctgtct gaattgaagt ttagtttttt ggatatgaaa aaacccgggt
      481 taaaaagctg gcctttgtaa ctgtattttt ctaccgaatg gctgtcaata ttcattgagt
      541 gctgccatcg gttaataaga tcgacggctg tatcaatagt agaaggatcg cgagtctgtg
      601 ttgcatcaat aatcatcgat ggaagttctt gtagttgcgt tttgcaaaat aattcaaata
      661 aatctgaatt ctctgtattg cagaaacgaa agtttatgct ttcttgatat tgctcccaaa
      721 cagtgatata ggttcttaat atacgattat aacgtgaacg aagcgaggca tttaagagtg
      781 attggttgtt tcggaaccat tctgttaata ttgaccattg tgaacgagta attcccatat
      841 agatacaaag ttcattgctg ataagatgtt ttctattat
//
LOCUS       DAZPEB010000417          876 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZPEB010000417 DAZPEB010000000
VERSION     DAZPEB010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.423"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            45..876
                     /locus_tag="ACHURJ_004366"
                     /pseudo
     CDS             45..876
                     /locus_tag="ACHURJ_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="programmed frameshift; internal stop; incomplete;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element ISSfl7 family transposase"
BASE COUNT          222 a          221 c          234 g          199 t
ORIGIN      
        1 ggacagcggt atcgtctggt ttttcaattt cctatacgtg aactatggct cgctacgacc
       61 ttcccgatga ggcatggacc atcatcaagc ctctgttacc tcctgaaccc gccacaccac
      121 gggccggacg cccatgggct gagcaccgta aaatcatcaa tggcatgttc tgggtgttat
      181 gttccggtgc accatggcgc gatttacccg aacgatatgg ggcatggaaa actgtttata
      241 accgctttaa ccgatgatca aagtcaggcg tgattaatat tattttcaac aggttgcttt
      301 cgctacttga tgcaaacggc tttattgact ggtctgccac cgcgctggat ggcagtaata
      361 tccgggcgct gaaatgtgcc gccggtgctc aaaaaaacat cccgatatcg acggagataa
      421 tggcccgggt cgctctcgcg gcggttttgg caccaaaatc catctggcaa cagacggaag
      481 tggcctcccg ttaaatatcg tgctaagccc cggacaagct catgaaagcc agttcgcgca
      541 acgacttctg gacgggattg gcgttcagcg tcagaacggc agcatgaaac gccgtggaca
      601 tgcggtactg gctgataaag cgtattcagg gcgcgcattg cgcaacgagc tgaaaaataa
      661 cggtataaag gcagtaatcc cccgaaagtc aaatgagaaa atggcatcgg atggacgtgc
      721 acagcttgat cgtgatgcgt actgcaatcg taatgtcgtt gaacggtgct ttgggcggct
      781 gaaagaatat cgccgcatcg ccacgcgtta cgacaaaacg gcgagaaatt acctggcgat
      841 ggtgaaactg ggctgcatcc gactctttta tcaacg
//
LOCUS       DAZPEB010000418          875 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZPEB010000418 DAZPEB010000000
VERSION     DAZPEB010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..875
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.424"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            4..765
                     /gene="ydeO"
                     /locus_tag="ACHURJ_004367"
     CDS             4..765
                     /gene="ydeO"
                     /locus_tag="ACHURJ_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFR1283027.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFTENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQSHTGMNTGNTMSALAI"
BASE COUNT          263 a          172 c          175 g          265 t
ORIGIN      
        1 aaaatgtcgc tcgtttgttc tgttatattt attcatcatg ccttcaacgc taacatttta
       61 gataaagatt acgccttctc tgacggcgag atcctgatgg tagataacgc tgttcgtacg
      121 cattttgaac cttatgagcg gcattttaaa gagatcggat ttactgaaaa taccattaaa
      181 aaatatctac aatgcactaa catccagaca gtgacggtgc ctgttcctgc gaagttttta
      241 cgtgcttcaa atgtaccgac tggattgctt aatgaaatga ttgcttatct caactcggaa
      301 gaacgcaatc atcataattt ttcagaactt ttgctttttt cttgcctgtc tatttttgcc
      361 gcatgcaaag gtttcattac actattaact aacggtgtgc tatccgtttc cggaaaagtg
      421 agaaatattg tcaacatgaa gctggcgcac ccatggaagc tgaaagatat ttgtgactgc
      481 ctgtacatca gtgaaagcct gttgaagaaa aaactaaagc aagagcaaac gacattctca
      541 cagattctat tagatgccag aatgcagcac gcaaaaaatt tgatacgcgt agaaggttca
      601 gtcaataaaa ttgccgaaca atgtggttat gccagtactt cttattttat ttatgcgttc
      661 cgcaaacatt tcggcaacag tccgaagaga gtttctaagg agtaccgttg tcaaagtcac
      721 acgggtatga atacgggcaa cacgatgagt gctttagcta tttgattatt tgctaacgag
      781 tagtcaacca cgcacgctac gtaagaatta tatggggcag ccattccctg ccccgcgttg
      841 tttttaggcg atatatttat tgaaataaat aagtg
//
LOCUS       DAZPEB010000419          869 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZPEB010000419 DAZPEB010000000
VERSION     DAZPEB010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..869
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.425"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            107..343
                     /gene="ycgZ"
                     /locus_tag="ACHURJ_004368"
     CDS             107..343
                     /gene="ycgZ"
                     /locus_tag="ACHURJ_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein YcgZ"
                     /protein_id="HFR1283028.1"
                     /translation="MHQNSVTLDSAGAITRYFAKANWPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEHATGEEEQKHYNALIGLLFE"
     gene            386..658
                     /locus_tag="ACHURJ_004369"
     CDS             386..658
                     /locus_tag="ACHURJ_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HFR1283029.1"
                     /translation="MKTSDNERIKYEITGQVVLQILRMKINFSLQTLIKQLLVMKSAE
                     EDAFRRDLIDSIIRDFSNSDSGGPNRRTATADNKSMFNGKKINRIH"
     gene            687..>869
                     /locus_tag="ACHURJ_004370"
     CDS             687..>869
                     /locus_tag="ACHURJ_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1283030.1"
                     /translation="MLEDTTIHNAITDKALASYFRSSGNLLEEESAVLGQAVTNLMWI
                     CPYISRHLLSLNPLQAR"
BASE COUNT          287 a          153 c          178 g          251 t
ORIGIN      
        1 gcactaattg caaaaaatta atttatcatt ctgtacacat atttcgtaca agtttgctat
       61 tgttacctca cttaacattg attaacattt ttaacagagg cgtagcatgc atcaaaattc
      121 agtgacttta gattctgcgg gagcaatcac tcgttacttt gcaaaagcta actggcctac
      181 tcagcaggaa actctcgggg aaattgtgac tgagattttg aaagatggac gtaatctgag
      241 tcgaaagtcg ctttgtgcca aacttctttg ccgactggaa cacgcgactg gggaagagga
      301 acagaaacat tataatgcac taattgggtt gctttttgaa taatgcaaca gcctgattat
      361 ttaacaggtt agctatgaag tcgttatgaa gacatctgat aatgaacgta taaaatatga
      421 aattactggc caggtggtgc tccagatact gcgcatgaag ataaattttt cattgcagac
      481 tcttattaag caattactcg taatgaaatc ggctgaggaa gatgctttcc gacgcgattt
      541 aattgacagc ataattcgtg attttagcaa tagtgattca ggagggccaa accgaagaac
      601 agcgacagct gacaataaaa gtatgttcaa tggtaagaaa ataaacagaa tacattaaaa
      661 tttcataagt aagatgagag gttaccatgc ttgaagatac tacaattcat aatgcaataa
      721 ctgataaagc gttagcaagt tactttcgca gttcgggtaa tttgttagaa gaagagtcag
      781 cagtgttagg gcaggctgtc accaatttaa tgtggatttg cccctatatt tccagacatc
      841 tgttatcact taacccatta caagcccgc
//
LOCUS       DAZPEB010000420          866 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZPEB010000420 DAZPEB010000000
VERSION     DAZPEB010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..866
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.426"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..360
                     /locus_tag="ACHURJ_004371"
     CDS             <1..360
                     /locus_tag="ACHURJ_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1283031.1"
                     /translation="TPVALSANDPANVWGNLLPWPAHPATLVPTRRAGALVVVSSGKL
                     LLYLAQGGKKMLVWQEKEELLAPEVFHALTTALRREPRLRFTLTEVNDLPVRQTPMFT
                     LLREAGFSSSPQGLDWG"
     gene            complement(411..758)
                     /gene="grxD"
                     /locus_tag="ACHURJ_004372"
     CDS             complement(411..758)
                     /gene="grxD"
                     /locus_tag="ACHURJ_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFR1283032.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
BASE COUNT          187 a          227 c          234 g          218 t
ORIGIN      
        1 acaccagtgg cgctctctgc caacgatccg gctaatgtgt ggggaaatct tctgccctgg
       61 cctgcacatc cggcaacgct ggttccaacg cgtcgggcgg gtgcgctggt ggtcgtttct
      121 agcggcaaat tgttactcta tctggcgcaa ggtggcaaaa aaatgctggt ctggcaggaa
      181 aaagaggaat tactcgcccc agaggttttc cacgcgctga ctaccgcact gcgtcgcgaa
      241 ccacggctgc gctttacgct aacagaagtg aatgatctac cggtccggca aacgccgatg
      301 tttacgctgc tgcgtgaggc gggattttca agttcgccac aagggctgga ttggggatag
      361 agaaaggact gacggatgcc cgttcgcatc cgtcagtatt gcaggacgga ttattccgcg
      421 tccggctctt cagacttgta tttagcggca gtttctttga tcagctgctg cagttcgcca
      481 cgctgataca tttcgatcac gatatcacaa ccgccgacca gctcgccgtc aacccacagt
      541 tgcgggaagg tcggccagtt agcatatttc ggcagttccg cacgaatgtc cggattctgc
      601 agaatatcaa cataggcaaa acgttcgcca catgcggcaa gcgcctggac tgcctgggca
      661 gagaaaccgc agctcggcag tttcggtgaa cctttcatgt acagcaggat cgggttttca
      721 gcaatctggc gttggatttt ttcgatagtg gtgctcattg tcttgcttcc tcaaacttct
      781 ttacggcagt aatctgacat tgtagcgggt cagtgcggca tcggaaaata acattttcat
      841 cacgcttttg ctattttatc cctttg
//
LOCUS       DAZPEB010000421          857 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZPEB010000421 DAZPEB010000000
VERSION     DAZPEB010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..857
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.427"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(32..112)
                     /gene="csgC"
                     /locus_tag="ACHURJ_004373"
                     /pseudo
     CDS             complement(32..112)
                     /gene="csgC"
                     /locus_tag="ACHURJ_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="involved in autoagglutination of curliated cells;
                     not involved in production of curli fibers; incomplete;
                     partial in the middle of a contig; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
     gene            complement(171..626)
                     /gene="csgA"
                     /locus_tag="ACHURJ_004374"
     CDS             complement(171..626)
                     /gene="csgA"
                     /locus_tag="ACHURJ_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFR1283033.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(667..>857)
                     /gene="csgB"
                     /locus_tag="ACHURJ_004375"
     CDS             complement(667..>857)
                     /gene="csgB"
                     /locus_tag="ACHURJ_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="CsgB; functions as a nucleator in the assembly of
                     curli (coiled surface structures) on the cell surface;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HFR1283034.1"
                     /translation="LAYIDQAGSANDASISQGAYGNTAMIIQKGSGNKANITQYGTQK
                     TAIVVQRQSQMAIRVTQR"
BASE COUNT          226 a          213 c          189 g          229 t
ORIGIN      
        1 gaacaaatgg tagtgtccgc tattgccagt acacctcata cacatcccct tgctgggtcg
       61 tattaaaggt tatctgactg gaaagtgccg caaggagtaa taacgcattc atattcttct
      121 cccgaaaaaa aacagggctt gcgccctgtt tctgtaatac aaatgatgta ttagtactga
      181 tgagcggtcg cgttgttacc aaagccaacc tgagtcacgt tgacggagga gttagatgca
      241 gtctggtcaa ctgcagcacc gttgccacca ccgaactgtt taaccgtcat ttcagaattt
      301 ttgccgttcc actgatcaag agtagcgcta ttaccgaagc cacgttgggt cagatcgatt
      361 gagctgtcat ctgagccctg accaacatct gcaccattac cgccgccatg ctgggtaata
      421 gtcaagtcag agttacgggc atcagtttgc agagcaagtg cagagttacc gccaccgtac
      481 tggtaaatgt tcagctcaga atttgggccg ctattattac cgccaccacc gtggttaccg
      541 ccgccgccgt actgaggaac aacacctgcc agagcgctac cggagaatac gattgctgca
      601 attgctgcta cttttaaaag tttcatgtaa aacccccatc ggattgattt aaaagtcgaa
      661 tggaaattaa cgttgtgtca cgcgaatagc catttgcgac tgtctctgca ctacaattgc
      721 cgttttttga gtaccatact gtgtaatatt tgctttatta ccagaacctt tctggataat
      781 catcgcagta ttaccataag caccttgcga aatactggca tcgttggcac tgcccgcctg
      841 atcaatatat gcaaggt
//
LOCUS       DAZPEB010000422          840 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZPEB010000422 DAZPEB010000000
VERSION     DAZPEB010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..840
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.428"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..246
                     /locus_tag="ACHURJ_004376"
     CDS             <1..246
                     /locus_tag="ACHURJ_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR1283035.1"
                     /translation="VFITVIADHDKPQPSGCLLESQGSLCPICRQRITHETGWNVHHK
                     VKKVMGGGEELSNLVLLHPNCHRQLHSCEAGSLKKGL"
     gene            389..610
                     /locus_tag="ACHURJ_004377"
                     /pseudo
     CDS             389..610
                     /locus_tag="ACHURJ_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(631..693)
                     /locus_tag="ACHURJ_004378"
                     /pseudo
     CDS             complement(631..693)
                     /locus_tag="ACHURJ_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          255 a          206 c          194 g          185 t
ORIGIN      
        1 gtttttataa cggtaattgc agaccatgac aaaccgcagc cgtcaggctg cctactcgag
       61 tcacaaggct ctctgtgtcc gatatgccgg cagcgcatta cccatgaaac tggctggaac
      121 gtacaccata aggtgaagaa agttatgggg ggcggtgaag aattatctaa ccttgtcttg
      181 ttacatccaa attgccatcg acagttacac agttgtgaag ccggctctct caaaaagggc
      241 ttataaaggc ttgagcggtg taccgggaaa ccggtatgca ccgttcttag gggagggcag
      301 tccagcaatg aactgtcctt acccgacaaa catccaaaac gaacggaacg tgctggaatc
      361 cgcatataac ccgttgtaaa gtgaggaaat ggacacctca cttgctcatg agaacgcccg
      421 cctgcgggca ctgttgcaga cgcaacagga cactatccac cagatggctg aatacaaccg
      481 cctgctctca cagcggatgg cggcttatgc ttccgaaatc aaccgactga aggcgctggt
      541 tgcgaaactg cagcgtatgc agttcggtaa aagcccagaa aaacttcgcg caaaaaccga
      601 acggcagatt ccattttcac gggcgatcta cgccacacag gctctgggtt gcagtgactg
      661 ctccattaag gcgattttga actcataagg gaagtaacaa aaaaactgca aaagtacaat
      721 aattatatat ggccaaacta acctctatta aaaaaacatc agtttttcac acttagttga
      781 gcttcatgaa aaaatcatcg aggatttcat tgcagaatac acattccact aactggaagc
//
LOCUS       DAZPEB010000423          839 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZPEB010000423 DAZPEB010000000
VERSION     DAZPEB010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.429"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            54..770
                     /locus_tag="ACHURJ_004379"
     CDS             54..770
                     /locus_tag="ACHURJ_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR1283036.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          283 a          143 c          171 g          242 t
ORIGIN      
        1 acatgttaac gatttaccag taatgtaaat aaattttcga ggagatcatt ccagtgggac
       61 gtaaatgggc caatattgtt gctaaaaaaa cggctaaaga cggtgcaacg tctaaaattt
      121 atgcaaaatt cggtgtagaa atctatgctg ctgctaaaca aggtgaaccc gatccagaat
      181 taaacacatc tttaaaattc gttattgaac gtgcaaagca ggcacaagtt ccaaagcacg
      241 ttattgataa agcaattgat aaagccaaag gcggcggaga tgaaacgttc gtgcagggac
      301 gttatgaagg ctttggtcct aatggctcaa tgattatcgc cgagacattg acttcaaatg
      361 ttaaccgtac gattgctaac gttcgcacaa ttttcaataa aaaaggcggc aatatcggag
      421 cggcaggttc tgtcagctat atgtttgaca atactggtgt gattgtattt aaaggatcag
      481 accctgacca tatttttgaa attttacttg aagctgaagt tgatgttcgt gatgtgactg
      541 aagaagaagg taacattgtt atttataccg aacctactga ccttcataaa ggaatcgcgg
      601 ctctaaaagc agctggaatc actgagttct caacaacaga attagaaatg attgctcaat
      661 ctgaagttga gctttcccca gaagatttag aaatctttga agggcttgtt gatgcccttg
      721 aagatgacga cgatgtacaa aaagtttatc ataacgtcgc aaatctctaa ttatatatta
      781 aagaaatctg tctttatggc agatttcttt aatctcatat aatccttata aaaaatata
//
LOCUS       DAZPEB010000424          828 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZPEB010000424 DAZPEB010000000
VERSION     DAZPEB010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..828
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.430"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..200)
                     /locus_tag="ACHURJ_004380"
     CDS             complement(<1..200)
                     /locus_tag="ACHURJ_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate transporter"
                     /protein_id="HFR1283037.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQMKWLA
                     ENNWKTLSSDELEFFYRGGKLPR"
BASE COUNT          248 a          192 c          159 g          229 t
ORIGIN      
        1 cgggggagtt ttccgccccg ataaaaaaac tccagttcat ctgatgacag tgttttccag
       61 ttattttcag ccaaccattt catttgcttc cggaatgtca cgggagaaag tgtaaccagt
      121 ccggggcaat gactgacatg atgatacatt aataccggaa catgattggc tttgaacaaa
      181 atgccccctt cgtttaacat ttaaagtttc tggcacctag taagcagaga caaaaggaaa
      241 acccaataaa gctggtgcat tttatttcca gatactggat gaataacata gcaaacccat
      301 ggcatactcg atatatttta ctaaattaaa catcaacaat tcataaacaa tgatatatta
      361 tcatatcttt taaaaacgac tcactaagaa ccttgaggtg gcacactgaa tttggctacc
      421 tgaacagagg tgatatgttc acctcagaac aacacaggtg ctccaatgaa aaaaaggaat
      481 ttcagcgcag agtttattta cggggtgacg ctttcattaa cctgtacacg aacttctatt
      541 ccggcatcat gacaggcctg cagctactgc gccatttcca gcggatcgcc ctcccggcgt
      601 accaccccac cttctttatt ccataactgc agagcatttt tctgattgtt ccatctgatt
      661 tcggggggta ttccgggcaa ggttgttcag taaggcattc cttcgtaaaa tcagcatgtt
      721 ttttgcacga cggaaaccgg cagccatgaa aatctgtctt tatcatactc tgaatcctga
      781 cacgatcccg ggttataaaa aatttgcaca ggctattgcc accgataa
//
LOCUS       DAZPEB010000425          825 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZPEB010000425 DAZPEB010000000
VERSION     DAZPEB010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.431"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            9..800
                     /gene="fdrA"
                     /locus_tag="ACHURJ_004381"
                     /pseudo
     CDS             9..800
                     /gene="fdrA"
                     /locus_tag="ACHURJ_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308607.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
BASE COUNT          198 a          192 c          253 g          182 t
ORIGIN      
        1 gagttgttat gatccacgcc tttattaaaa aagggtgttt tcaggattcg gtcagtttaa
       61 tgattatttc acgaaaactc agcgaatcag aaaatgttga tgatgtttcc gtaatgatgg
      121 gtacgcccgc caataaagcg ttattagata ccacaggttt ctggcatgac gattttaata
      181 acgccacgcc gaacgatatt tgcgtggcaa ttcgtagcga agcggcggat gcggggatcg
      241 cgcaggcgat tatgcagcag cttgaagagg cgctaaaaca actggcgcag ggggcaggca
      301 gcagccagtc cttgacgcag gtgcgtcgct gggacagtgc ctgtcagaaa ttatccgatg
      361 ccaatctggc gctgatttca gtggctggcg agtatgcggc ggagctggcg aatcaggcgc
      421 tggagcgcaa cctcaacgtg atgatgttct ccgataacgt cacgctggaa gatgaaatcc
      481 aacttaaaac ccgcgcgcga gaaaaaggct tgctggtgat ggggccggac tgcggtacgt
      541 cgatgattgc cggcacgccg ctggcttttg ctaacgtgat gccggaaggc aacattggcg
      601 tcattggcgc ttccggtacc gggattcagg aactgtgttc gcagattgcg caggcagggg
      661 agggaattac tcacgcgatt ggtcttggcg agcgcgacct cagccgtgaa gtaggcggca
      721 tcagtgcgct gacggcgctg gaaatgctca gtgcagacga gaaaagcgaa gtgctggcat
      781 ttgtttcaaa gccacctgcc tagactggcc ccctgaatct ccaga
//
LOCUS       DAZPEB010000426          821 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZPEB010000426 DAZPEB010000000
VERSION     DAZPEB010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..821
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.432"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..>821
                     /locus_tag="ACHURJ_004382"
     CDS             <1..>821
                     /locus_tag="ACHURJ_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFR1283038.1"
                     /translation="DVLRQYVLMPGKVHADDIPVPVQEPGSGKTRTARLWVYVRDDRN
                     AGSQMPPAVWFAYSPDRKGIHPQNHLAGYSGVLQADAYGGYRALYESGRITEAACMAH
                     ARRKIHDVHARVPTDITTEALQRIGELYTIEAEVRGCSAEQRLAARKARAAPLMQSLY
                     DRIQQQMKTLSRHSDTAKAFAYLLKQWKALNVYCSNGWVEIDNNIAENALRGVAVGRK
                     NWLFAGSDSGGEHAVVLYSLIGTCRLNNVEPEKWLRYVIEHLQDWPANRVRDLLP"
BASE COUNT          202 a          216 c          247 g          156 t
ORIGIN      
        1 tgacgtcctg cgccagtatg tgctgatgcc cggtaaagtt catgcggatg atatccccgt
       61 tccggtccag gagccgggca gtggtaaaac aagaactgcc cggctgtggg tctatgtccg
      121 tgatgaccgt aacgccggtt cacagatgcc cccggcggtc tggttcgcgt acagcccgga
      181 ccggaaaggt atccatccac agaatcacct ggctggttac agcggtgtgc ttcaggcaga
      241 tgcttacggt ggttaccggg cgttatacga atccggcaga ataacggaag ccgcgtgtat
      301 ggcccatgcc cggagaaaaa tccacgatgt gcatgcaaga gtgccaaccg acatcaccac
      361 ggaagccctg cagcgtatcg gtgaactgta taccatcgag gcagaagtcc ggggatgttc
      421 agcagaacag cgtctggcgg caagaaaagc aagagccgcg ccactgatgc agtcactgta
      481 tgaccggata cagcaacaga tgaaaacact gtcgcgtcac tcggatacgg caaaagcgtt
      541 cgcataccta ctgaaacagt ggaaggccct gaacgtgtac tgcagtaatg gctgggtgga
      601 aatcgacaac aacatcgcag agaacgcctt acggggagtg gccgtaggcc ggaaaaactg
      661 gctcttcgcg ggttccgaca gcggtggcga acatgcggtg gtgttgtact cgctgatcgg
      721 cacatgccgt ctgaacaatg tagagccaga aaaatggctg cgttacgtta ttgagcatct
      781 ccaggactgg ccggcaaacc gggtacgcga tctgttgccc t
//
LOCUS       DAZPEB010000427          820 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZPEB010000427 DAZPEB010000000
VERSION     DAZPEB010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..820
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.433"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            122..>820
                     /gene="yfdF"
                     /locus_tag="ACHURJ_004383"
     CDS             122..>820
                     /gene="yfdF"
                     /locus_tag="ACHURJ_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdF"
                     /protein_id="HFR1283039.1"
                     /translation="MLPSISINNTSATYPESINENNNDEVNGLVQELKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRFNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGAPSFIFDKISHKLRHTVTEF
                     DESGKSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSENARSASA"
BASE COUNT          313 a          146 c          136 g          225 t
ORIGIN      
        1 ggctattgtg cgctcataca ctcaaattaa agataggttc taaataaatg agcgtttttt
       61 gatagtctat ttcattaggt aatatatatt tgtaacaaat caatcaaaat ggaataaaat
      121 catgctacca tctatttcaa tcaacaatac cagcgcaact tacccagaat ccattaatga
      181 aaataacaat gatgaagtta atggattagt acaagagctc aaaaaccttt ttaatggtaa
      241 ggaaggaata agcacctgta ttaaacatct acttgagctt ataaaaaacg ccatacgagt
      301 aaacgacgat ccttatagat ttaatattaa taattcctca gttacttata ttgatattgg
      361 ctccaatgat acagaccata ttactattgg tatcgacaac caagaaccaa tagaattacc
      421 tgcgaactat aaagacaaag aactcgtccg tactatcatt aatgacaaca tagttgagaa
      481 gactcatgat atcaataaca aggaaatgat cttcagcgca ttaaaagaaa tatatgatgg
      541 agctcctagt tttatcttcg ataagatatc acacaaactc agacatacgg taacggaatt
      601 tgatgagagc gggaaaagcg aaccaacgga cttatttacc tggtacggta aagataaaaa
      661 aggcgactct ctcgctattg taattaaaaa taaaaacgga aatgattact tatccctcgg
      721 ttactacgat caggacgact accacattca aagaggaatt cgtattaatg gtgatagtct
      781 cacccaatat tgtagtgaaa acgccaggag tgcttcagcg
//
LOCUS       DAZPEB010000428          815 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZPEB010000428 DAZPEB010000000
VERSION     DAZPEB010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.434"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            187..342
                     /locus_tag="ACHURJ_004384"
                     /pseudo
     CDS             187..342
                     /locus_tag="ACHURJ_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(372..>815)
                     /gene="ipaH7.8"
                     /locus_tag="ACHURJ_004385"
     CDS             complement(372..>815)
                     /gene="ipaH7.8"
                     /locus_tag="ACHURJ_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HFR1283040.1"
                     /translation="EIEVYLAFQTMLAEKLQLSTAVKEMRFYGVSGVTANDLRTAEAM
                     VRSREENEFKDWFSLWGPWHAVLKRTEADRWAQAEEQKYEMLENEYSQRVADRLKASG
                     LSGDTDAEREAGAQVMRETEQQIYRQLTDEVLALRLSENGSNHIA"
BASE COUNT          206 a          221 c          195 g          193 t
ORIGIN      
        1 acagagagca gaacgggatc tatacttttg cgggtcaaca taagaggatg agtccttaat
       61 aaaacaggtc ctcagagaat acatattctg cggagcgtgc ggcaacaaca ggacgaacag
      121 gggcggtttt caatttgcag ccgtcaggct gccgtggttc actccaacaa cggagacagg
      181 taaattatgg acgaaaagaa gcttaaagcc cttgcggctg aactggctaa aggcctcaaa
      241 accgaggccg acctcaatca gttttcccgt atgcagacga aattaactgt cgaaacggtg
      301 cttaatgagg aactaactga ccatctcggg catgaaaaaa gctacatccg gtagtaaaca
      361 ttatcagtgg gttatgcgat atgatttgag ccgttttcag acaatcgcag ggccagtacc
      421 tcgtcagtca actgacggta aatctgctgt tcagtctcac gcatcacctg tgcaccggct
      481 tccctctccg catccgtatc accgctcaga cctgatgctt tcagccggtc agccaccctc
      541 tgagagtact cattctccag catctcatac ttctgctctt ctgcctgcgc ccagcggtca
      601 gcttccgtac gcttcagtac agcatgccat ggtccccaga gggagaacca gtccttaaat
      661 tcattctctt cacggcttct gaccatggct tcggcagtgc ggaggtcatt tgctgtcact
      721 cccgacacgc catagaaacg catttccttc acggcagtgg agagctgaag tttctctgcg
      781 agcatggtct ggaaggccag gtagacttct atctc
//
LOCUS       DAZPEB010000429          815 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZPEB010000429 DAZPEB010000000
VERSION     DAZPEB010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.435"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..128
                     /locus_tag="ACHURJ_004386"
     CDS             <1..128
                     /locus_tag="ACHURJ_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1283041.1"
                     /translation="DAEREAGAQVMRETEQQIYRQLTDEVLALRLSENGSQLHHS"
     gene            336..605
                     /gene="yacA"
                     /locus_tag="ACHURJ_004387"
     CDS             336..605
                     /gene="yacA"
                     /locus_tag="ACHURJ_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015056448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin YacA"
                     /protein_id="HFR1283042.1"
                     /translation="MAQVNMSVRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNSWFRDQVAAGRQQLECGDVLPHDMVESSAAAWRDEMSRKIADK"
     gene            605..815
                     /locus_tag="ACHURJ_004388"
                     /pseudo
     CDS             605..815
                     /locus_tag="ACHURJ_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009346.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
BASE COUNT          208 a          189 c          237 g          181 t
ORIGIN      
        1 cggatgcgga gagggaagcc ggtgcacagg tgatgcgtga gactgaacag cagatttacc
       61 gtcagctgac tgacgaggta ctggccctgc gattgtctga aaacggctca caactgcacc
      121 attcataatc acatcgcata aaactgtcag cgccagtgat aggacaggtg aaacctgtca
      181 atacgtaccg gcttacgccg ggcttcggcg gttttactcc tctgccatat gcaacaacgg
      241 agtgccgcct tacatgccgc tggcgcggca tatcctggtg acgatatctg aacagttata
      301 tactgtgtat atacgtggta atgacgaggt ggcaaatggc acaggttaat atgagtgtaa
      361 gaatcgacgc agaactgaag gatgctttta tggccgctgc aaaaagcatg gaccgtaatg
      421 gctctcagtt aatccgggat tttatgcgcc agaccgttga acggcagcac aattcttggt
      481 tccgtgacca ggttgcggca ggccgtcagc aactcgaatg cggtgatgtg cttccccatg
      541 acatggtcga atcctctgct gctgcatggc gggatgaaat gagcaggaag attgctgata
      601 aatgatggag atgttctgga ccatgctggc catccaggac aggaagcgca ttcgtagtac
      661 attgcggagc agaacctgat ggccgccatt gaaccggacg aacggattgg ttattcagcc
      721 agcagcctgg cagggcagcc gtataaaggc cgcaacggtc gtgtggaagg aacatctgga
      781 ccacacaagg tagcgtgtaa cgttattttg tgtga
//
LOCUS       DAZPEB010000430          812 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZPEB010000430 DAZPEB010000000
VERSION     DAZPEB010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..812
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.436"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..>812
                     /locus_tag="ACHURJ_004389"
     CDS             <1..>812
                     /locus_tag="ACHURJ_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFR1283043.1"
                     /translation="CEHLPLYRQSEIFARQGVELSRALLSNLVDACCQLMTPLNDALY
                     RYVMNSRKVHTDDTPVKVLAPGRKKAKTGYIWTYVRDDRNAGSPEPPAVWFAYSPDHQ
                     GKHPEQHLSPFRGILQADAFNGYDRLFSAEREGGALTEAGCWAHARRKVHDVYISTKS
                     ATAEEALKLIGELYAIEHEIRGLPVSERLAVRQMQSKPLLTSLYKLMQEKEHTLSKKC
                     RLRDAFRYIRKHWVALCNFSDDGLAEADNNAAERALRAVCLGKKNFMFFGSD"
BASE COUNT          207 a          196 c          236 g          173 t
ORIGIN      
        1 tgcgaacacc tgccactgta tcgtcagagt gaaatttttg cccgtcaggg tgtcgaactg
       61 agccgtgcat tactctccaa cttggttgac gcgtgctgcc agttaatgac gccgctgaat
      121 gatgctctgt accgttatgt aatgaacagc cgcaaagttc acactgatga cacaccagta
      181 aaagtgctgg caccgggcag gaagaaggcg aaaacaggat atatctggac gtatgtccgg
      241 gatgacagga atgccggttc gccagagcct ccggcggtct ggttcgccta ctcaccggac
      301 catcagggta aacatccgga gcagcacctt agtcccttcc ggggtatcct gcaggcagat
      361 gcgtttaatg gttacgatcg gctgttcagt gccgaacgag aaggcggcgc gttgacggaa
      421 gcaggatgct gggctcatgc gcggcgcaaa gtccacgatg tatatatcag taccaaaagc
      481 gcgacagcgg aagaagccct gaaactaatc ggtgagctgt acgccatcga gcacgaaata
      541 cgcgggttgc cggtgtctga acgcctggcg gtcaggcaaa tgcagagtaa accgctactg
      601 acttccctgt ataagctgat gcaggagaaa gaacacacgt tatcgaaaaa atgccgtctg
      661 agagatgcgt tccggtatat caggaagcac tgggttgcgt tgtgcaactt cagtgatgat
      721 ggtctggctg aggcggataa taatgccgcg gaaagagcgc ttcgtgcagt ctgtctcgga
      781 aagaaaaact ttatgttctt cggcagcgat ca
//
LOCUS       DAZPEB010000431          808 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZPEB010000431 DAZPEB010000000
VERSION     DAZPEB010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..808
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.437"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..>808)
                     /gene="ipaH9.8"
                     /locus_tag="ACHURJ_004390"
     CDS             complement(<1..>808)
                     /gene="ipaH9.8"
                     /locus_tag="ACHURJ_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HFR1283044.1"
                     /translation="SFYNTISGTYADYFSAWDKWEKQALPGEERDEAVSRLKECLINN
                     SDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKLYSASNKLSELPVL
                     PPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALKNLRATRNFLTELP
                     AFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSSHALQALQRLTSSP
                     DYHGPRIYFSMSDGQQNTLHRPLADAVTAWFPENKQSDVSQIWHAFEHEE"
BASE COUNT          219 a          144 c          202 g          243 t
ORIGIN      
        1 tgctcttcat gttcaaaagc atgccatatc tgtgatacat cagattgttt gttttccggg
       61 aaccatgctg tcacggcatc agccaggggg cgatggagtg tattctgttg tccgtcactc
      121 atggagaagt aaatccgtgg gccgtggtag tccggcgaag aggttaatct ttgcagggct
      181 tgcagagcat gggatgataa tgggttatca ctaatatgta ttgaacattc attcctcaga
      241 ttaagaatgc tttccgggat atgacttatc tgatttctat caaaaaaata ctctctgaca
      301 acgggattat ttccctcaga aaatgctggt agctcagtga ggaaattacg ggtcgctctg
      361 agatttttaa gagcctgtgg gagcgatggt aaggagacta tttcgttata gctgatattc
      421 atagtcaata acgaatcggg taaagctggc aggttttcca gctcattgtg ttgtacctga
      481 agtgactcca gcgcaggagg tagcacgggc aattctgata atttattgct ggcggaatat
      541 aattttttta gcgtaacagg cagttcaggt aggttagtta attgattata tgatacattg
      601 agcagcgtta tctgagctgg taagttgtca ggtagcgagg acagatttaa acggtccagt
      661 cgaagttcat cggaattatt gataagacat tctttaagtc gggagacagc ctcatcacgc
      721 tcttcaccgg ggagcgcttg tttttcccat ttatcccatg ctgaaaagta atcagcatat
      781 gtaccggaaa tagtgttata aaaagaat
//
LOCUS       DAZPEB010000432          803 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZPEB010000432 DAZPEB010000000
VERSION     DAZPEB010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.438"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            65..803
                     /gene="tssD"
                     /locus_tag="ACHURJ_004391"
                     /pseudo
     CDS             65..803
                     /gene="tssD"
                     /locus_tag="ACHURJ_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278761.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
BASE COUNT          248 a          153 c          176 g          226 t
ORIGIN      
        1 aaggtaaaaa aaacaacaat actatttctc tttcacaaaa gaattaacaa aaggtttttt
       61 aaggatgagt gatattattt acttgaccat aaccggagaa caacaaggat gtatttcctc
      121 tctttgtgga acgagcgcat caataggtaa tcgctggcag attggccatg aagatgaaat
      181 cttcgcgttc tcgctctcta atagtattac gaatgcaggc aaagggtcac aattgcatgg
      241 ccttagtttc tgtaaattct tattaacgct attaacaata acgagcagtt gttcatggcg
      301 tttgacttct atcggatcaa tagatttggt cgatgggaga aatattacaa cattcaactc
      361 cgcggcgcgc tcttatcagc aatcaatcac ctgtttactg agaacaatct ggatacagaa
      421 gccatcactg tcagttatga atatatactc tccaggcatc tgattgctaa tactgaattt
      481 agttatttgg catttcccga taactacaac cgtctgttta taccacggcc aaaaactaaa
      541 gctggtaatg gttcaaaaca cttaatagta aagcggttgg cagactgctt gctgctggcg
      601 gcatatacaa tgggaatatc gaaggattca gggaaacagc agacaagttg gggggagatg
      661 cacctgctgg ttacgatcaa tttatggata acaaagggtt actcatcgct ggtgcttcaa
      721 tagttgctgg agcagttctg gggagaatga gactccctga tttggaatca cttgagcatt
      781 tcggagccag aggagcagta tca
//
LOCUS       DAZPEB010000433          800 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZPEB010000433 DAZPEB010000000
VERSION     DAZPEB010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..800
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.439"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..766
                     /locus_tag="ACHURJ_004392"
     CDS             <1..766
                     /locus_tag="ACHURJ_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001009620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1283045.1"
                     /translation="IPVKYVQAPSYKEIFEVLSETKKRIPAHLFRMIKDELYELVLTD
                     DPKGKLYVRDSEKIEEGVSTTEFVVGYGAISMVKKSESMATKGLVGLERVDLIREVVF
                     ENGYYDWGCVVNDVLPNICKGNARIPIFHFLNHAKLINPDGSIINETELSGGVLSRLN
                     ITPISFQSQGWDKRRSETVPEVKVGVNELYLAYDFSFFLRMMPYMEPGLIKRDMGELL
                     KILKKHIDEAMNTQVLSSNFCKLVCIYDYIKNSHRY"
BASE COUNT          262 a          106 c          182 g          250 t
ORIGIN      
        1 aatcccggta aaatatgttc aggcaccatc ctataaagaa atatttgagg tgctgagtga
       61 aactaaaaaa aggattccag cacatttatt ccgaatgata aaagatgaac tttatgagtt
      121 ggtcttaact gatgatccga aaggaaaatt atatgtgagg gattcagaga aaattgaaga
      181 aggggtcagt acaactgagt ttgttgtggg atatggtgct atttcgatgg taaaaaaatc
      241 ggaatcgatg gcaactaaag gattggttgg actggagcgt gtagatctca tccgagaggt
      301 tgtatttgaa aatggatatt atgattgggg atgtgttgtt aatgatgttt tgccaaatat
      361 atgcaaaggc aatgcgcgca ttcctatttt tcacttctta aatcacgcta aattaattaa
      421 tcctgatggg agtataatta atgaaacaga actctcaggt ggcgttttat cacgattaaa
      481 tatcactcct attagttttc agtctcaagg atgggataaa cgacgctctg aaaccgttcc
      541 tgaggtcaag gtgggggtca atgagttata tcttgcttat gatttcagtt tctttttaag
      601 aatgatgcct tatatggagc ctggattaat caagcgcgat atgggagagc ttctcaaaat
      661 attgaaaaaa catatagatg aagctatgaa tactcaggtg ttgtcaagta atttttgcaa
      721 attagtgtgt atttatgatt atatcaaaaa tagtcacagg tattaagtaa gatataacct
      781 tcttaggggg cgagatagta
//
LOCUS       DAZPEB010000434          787 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZPEB010000434 DAZPEB010000000
VERSION     DAZPEB010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.440"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            1..572
                     /locus_tag="ACHURJ_004393"
                     /pseudo
     CDS             1..572
                     /locus_tag="ACHURJ_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
BASE COUNT          204 a          207 c          181 g          195 t
ORIGIN      
        1 tacgcccaca gcacctaatc aggtctgggt cgcagattca gttgtccagg ccttccgaaa
       61 tcaaccgact gaaggcgctg gtcgcgaaac tgcagcgtat gcagttcggt aaaagctcag
      121 aaaaacttcg cgcaaaaacc gaacggcgga tacaggaagc acaggagcga atcagcgcac
      181 ttcaggagga aatggcggaa acactgggtg agcaatatga cccggtactg ccatcttccc
      241 tgcgccagtc ttcagcccgt aaaccgttac cagcctcact tccccgtgcc ccccgggtca
      301 tccggccgga agaggaatgc tgtccggcct gtggtggtga actcagtcca ctgggatgtg
      361 atgtgtcaga gcagctggag cttatcagca gcgcctttaa ggttatcgaa aaacaacgcc
      421 ctaaactggc ctgtcgccgg tgcgaccata tcgtgcaggc accggtgcct tcaaaaccca
      481 ttgcacgcag ttatgccgga gcggggcttc tggcccatgt tgttaccgga aagtatgcag
      541 accatctgcc attataccgc caaagtgatc tactattcca cacagctatt tagctctctt
      601 tttgttgagc tcccatgatt ctttcattta ttgttactaa ttattttatg ttatcatgat
      661 gttagaacac gctctgctct gtgtctctat tttatctatc atattctgag gattaactac
      721 ataacaaggc gcaagatata tatccaaaaa caaataatac cgttgcaata attcctttca
      781 gtaaagg
//
LOCUS       DAZPEB010000435          778 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZPEB010000435 DAZPEB010000000
VERSION     DAZPEB010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.441"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(32..736)
                     /locus_tag="ACHURJ_004394"
     CDS             complement(32..736)
                     /locus_tag="ACHURJ_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020324562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HFR1283046.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDV
                     EHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYY
                     GDPLGEIRLVSRVFEM"
BASE COUNT          178 a          207 c          200 g          193 t
ORIGIN      
        1 atgaggcagc cttttgtctt attcaaaggc cttacatttc aaaaactctg cttaccaggc
       61 gtatttcgcc caggggatca ccataataaa atgctgaggc ctggcctttg cgtagtgcac
      121 gcatcacctc aatacctttg atggtggcgt aagccgtctt catggattta aatcccagcg
      181 tggcgccgat tatccgtttc agtttgccat gatcgcattc aatcacgttg ttccggtact
      241 taatctgtcg gtgttcaacg tcagacgggc accggccttc gcgtttgagc agagcaagcg
      301 cgcgaccata ggcgggcgct ttatccgtgt tgatgaatcg cgggatctgc cacttcttca
      361 cgttgttgag gattttaccc agaaaccggt atgcagcttt gctgttacga cgggaggaga
      421 gataaaaatc gacagtgcgg ccccggctgt cgacggcccg gtacagatac gcccagcggc
      481 cattgacctt cacgtaggtt tcatccatgt gccacgggca aagatcggaa gggttacgcc
      541 agtaccagcg cagccgtttt tccatttcag gcgcataacg ctgaacccag cggtaaatcg
      601 tggagtgatc gacattcact ccgcgttcag ccagcatctc ctgcagctca cggtaactga
      661 tgccgtattt gcagtaccag cgtacggccc acagaatgat gtcacgctga aaatgccggc
      721 ctttgaatgg gttcatgtgc agctccatca gcaaaagggg atgataagtt tatcacca
//
LOCUS       DAZPEB010000436          778 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZPEB010000436 DAZPEB010000000
VERSION     DAZPEB010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.442"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            17..>778
                     /locus_tag="ACHURJ_004395"
     CDS             17..>778
                     /locus_tag="ACHURJ_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR1283047.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTLLQQTAQPDYHGPQIYFSMSDGQQNTLHR"
BASE COUNT          246 a          180 c          138 g          214 t
ORIGIN      
        1 cttctgcgag gttattatgc ttcctgtaaa taatcccccc ctatccactg gaaacgtctc
       61 tttttacaga actacatcaa tcgacaatgt tcacaataat tatctctccg aatgggttga
      121 atggactaaa aacagcattt ccggagaaaa cagggaaact gcttttaccc ggctccaatt
      181 atgtctggaa aacagtgaaa catcgttgga cttatcttgt ttaggtctca gatctctacc
      241 acgattgcct gacaatcttg atgaaattaa tgtaagcaat aaccaactat caatgctccc
      301 cgagctacca agggcattga aagaactgaa tgcaagcagt aatcaattat ctgcacttcc
      361 tgaattacca gtgtcgctgg aatatataaa tgtgagtgat aaccatttgt tcgcacttcc
      421 tgaattacct gcgtcactag aatatattaa cgtaagtgac aatcacctgt ctgtacttcc
      481 gaggttacca atgtcattgg aattacttga tgcagccaga aatgctttgg aagtaatacc
      541 agattttcca gaaagagatg atcatattat aagaatattc tggcttaatc agaaccggat
      601 cacggcaatt ccggaaagca tacttggcct cagttctgat agcgttgtca atcttagaga
      661 aaatcaacta tctcccagaa taatgcaaac tttgttacaa caaaccgccc aaccggacta
      721 ccacggcccg cagatttact tctccatgag tgacggacaa cagaatacac tccatcgc
//
LOCUS       DAZPEB010000437          763 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZPEB010000437 DAZPEB010000000
VERSION     DAZPEB010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.443"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(118..714)
                     /locus_tag="ACHURJ_004396"
                     /pseudo
     CDS             complement(118..714)
                     /locus_tag="ACHURJ_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
BASE COUNT          198 a          203 c          155 g          207 t
ORIGIN      
        1 ctgcttaaaa aaagaacaaa tggtagtgtc cgctattgcc agtacacctc acatggcggg
       61 ctatctgacc gccagcacca agaatcagta catttttcat aatatttctc atgaaactta
      121 tcccagatgg gggttgaaaa aaggaataac tttttccagc gcctcctgaa ctgcttccgg
      181 tttgtcatac agttcataat gtgagaaatc gggtaatgaa actaactgac gatcctgcga
      241 aaccatcgcc cggccataga cttccagtcc atcacgataa gcaccgaagg caccgacttt
      301 ttcaccaacg actaccatca ccggctgagt cagtaacact tcagtaaatg cgaaggcatc
      361 ccaggccagc gttttctgtg catgtgagaa caacattttt gtcgcacctc ccggttgttg
      421 cccgcgtggt gttttatagt agtcagttgc ttcataaaca tcgcgttcgg tcagtccatg
      481 ggcttttgct gcatccaggc tggcgggaag taattcgttg atctgtaatt ctccaccacg
      541 agcctctttg gttcgttgcg acgccattgc attcaatgcg ccaatcggat cgtaattgct
      601 gaaaccttcc cgaaaaagac gcccgatatt aacacccgta atactcacta ctgctttaat
      661 tcgtttttct gtcagcgcgg cattaatggc atagtcacca cccccacaca ctcccggtaa
      721 tgactccaac ttactgatag tgttttatgt tcagataatg ccc
//
LOCUS       DAZPEB010000438          758 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZPEB010000438 DAZPEB010000000
VERSION     DAZPEB010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..758
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.444"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..321)
                     /locus_tag="ACHURJ_004397"
     CDS             complement(<1..321)
                     /locus_tag="ACHURJ_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1283048.1"
                     /translation="MWNDIFTKLSIAILLAFSAYVHAETSAPIFFEDVGSIDSWFSIK
                     YQKYENVKDEQYPEILIYYGVKGSGINRVDGWFFSCAKAGRTCSLIFMADFGESRYFK
                     PAKIA"
     gene            complement(294..>758)
                     /locus_tag="ACHURJ_004398"
     CDS             complement(294..>758)
                     /locus_tag="ACHURJ_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFR1283049.1"
                     /translation="ITDPLGLIDKPAPVFNRDLNSDPYYLAVNNCYSYALNRYGNPGS
                     RIFGGGGLQPGELSGKEFSKLTCGSIFEASKNDGAKDLDNGSCPSGYHKAQLFIRPHN
                     FIGMGGDYHWYRQDANGEWSDKQGVGAIRFRGKDPFPPIDYPEKCGTIFLPN"
BASE COUNT          237 a          167 c          121 g          233 t
ORIGIN      
        1 agctattttt gcaggtttaa aatagcggct ttcaccaaag tcagccatga aaattagact
       61 acaagttctc ccagcctttg cacatgagaa aaaccaccca tcaactctat ttattcctga
      121 acctttaaca ccataataaa tcagaatttc tgggtattgt tcatccttca cgttttcata
      181 tttttggtat ttaattgaaa accaagagtc tatactgcca acatcttcaa aaaatattgg
      241 agctgatgtt tccgcatgaa catatgcaga aaatgctaac aaaatggcaa tacttagttt
      301 ggtaaaaata tcgttccaca cttttcagga taatctattg gggggaaggg atcttttcct
      361 ctaaatctaa ttgctcccac tccttgtttg tcagaccatt cgccatttgc atcctgacga
      421 taccagtgat aatcccctcc catgcctata aaattgtgag gacgaataaa caattgagct
      481 ttatggtaac cagatggaca actcccatta tctaaatcct tggcaccatc attttttgac
      541 gcttcaaaaa tactaccgca agtgagttta gaaaactctt ttccagataa ttctccaggt
      601 tgtaatcctc cgccgccaaa aatcctactc ccagggtttc catacctatt tagagcatat
      661 gaataacaat tatttacggc aagataatac ggatctgaat ttaggtctcg attgaatact
      721 ggagcaggtt tatcaattaa gcccagcgga tcggtaat
//
LOCUS       DAZPEB010000439          750 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZPEB010000439 DAZPEB010000000
VERSION     DAZPEB010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.445"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(9..641)
                     /gene="mxiE"
                     /locus_tag="ACHURJ_004399"
     CDS             complement(9..641)
                     /gene="mxiE"
                     /locus_tag="ACHURJ_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HFR1283050.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
BASE COUNT          246 a          142 c          102 g          260 t
ORIGIN      
        1 atttaatatt aaattttttc atttattttt ttcactaaaa aagtaatatt cgataattct
       61 cttgcagaaa agcctagtcg tgtttttatc tcatttgaaa aatgggatgt tgatgcatac
      121 ccattattca tggcagcaga agtgattgtt tgattgtgca aaaatacatc taataaacca
      181 tttactaacc gccaagtgtt cagttgttct ttgacttttg cccctaaagc ttttctacat
      241 aaagatctaa aataagcctc cgacacacca taatgctctg tcagactctt tattcttacc
      301 tccttttctt tctcaatagt tctcagaaga taaagaacca aatgatatga ctcagtcatt
      361 cgtatcatag ccagtgcatt acttacattc tggtcagatg aaaagatgaa tcttaacatt
      421 aacttatcaa gcatcgattc ggtattacaa ctatcccgaa gatcattctc atgatagaaa
      481 tgaatacatt tttcagcatg gctatgctct actagagcac cactgaaata gtcccaaaaa
      541 gcaagaaatt ttgcacaact attaatgtta aagcgataat gatagttcac attatcactg
      601 aattttatat tttgatttcg gacaaaaaaa aacccttcca tgctggagcc agctcaatta
      661 tgttacgttc tttatctttt acaagctcaa cgttaactgg tttatgtcca ctagagtaaa
      721 tgtaaaacat attacttgta tttagacctt
//
LOCUS       DAZPEB010000440          731 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZPEB010000440 DAZPEB010000000
VERSION     DAZPEB010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.446"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            94..312
                     /gene="tdcR"
                     /locus_tag="ACHURJ_004400"
     CDS             94..312
                     /gene="tdcR"
                     /locus_tag="ACHURJ_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator TdcR"
                     /protein_id="HFR1283051.1"
                     /translation="MSKFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNT
                     GIDHLEQLINVNFFSSDRTSFCECNRFP"
     gene            568..>731
                     /gene="yhaB"
                     /locus_tag="ACHURJ_004401"
     CDS             568..>731
                     /gene="yhaB"
                     /locus_tag="ACHURJ_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaB"
                     /protein_id="HFR1283052.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVKKIYSISN"
BASE COUNT          239 a          130 c          135 g          227 t
ORIGIN      
        1 atcattcgtt atgtggttaa tacaaaaaag gggctgagac catatttcaa gcaattaccg
       61 gataattacc aggcaaaatt tgagttaaat ttaatgagca agttctcaaa ttttattata
      121 aataaaccat tttcagcgat aaatactgcg gcacgtcaca ttttttcacg ctatttgttg
      181 gagaacaaac atttatttta tcaatatttt aaaatttcga atacaggtat tgatcatctc
      241 gaacaattga ttaacgtcaa ctttttctct tctgacagga cgtcattttg tgaatgcaat
      301 cgttttccat aaattcttct cccctcatag gcgacgaata gcattttgtg ttgaggatca
      361 caaaacaaat aattgctggt cgccgcgata aggtcagacg aagtcaacaa ggggaatttt
      421 cactgtgctt tacatcagcg taggacacag aatgttgctt cgtttactgt aacgccgggt
      481 aaatgagcgt tttttgatag tgcgtaagtg tcgtgcgagg aaaataagca tctattattg
      541 tttgtcatga cgacaaggca ggcatcaatg aaaggcttcc caattgcaca tatttttcac
      601 ccttcaatcc cgccaatgca cgcagtggtt aacaatcaca atagaaatat tgattattgg
      661 acggtaaaaa gaaagtttgc agaaattgtc tccaccaatg acgttaaaaa aatttacagt
      721 ataagtaatg a
//
LOCUS       DAZPEB010000441          726 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZPEB010000441 DAZPEB010000000
VERSION     DAZPEB010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.447"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            35..>726
                     /locus_tag="ACHURJ_004402"
     CDS             join(35..284,284..>726)
                     /locus_tag="ACHURJ_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR1283053.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFT
                     YTASQPGTHQKIIDMAMNGVGCRASARIMGVGLNTVLRHFKKLRPQSVTSRIQPGSDV
                     IVCAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEV
                     VVWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELH
                     DKVIGHYLNIKHY"
BASE COUNT          170 a          181 c          200 g          175 t
ORIGIN      
        1 atttagtgta tgatggtgat tttaaggtgc ttgcgtggct tccatttcca tcagatgtcc
       61 ttcctgctcc gctactgaag gcgtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcattgcc gtaaaacatg gcaactacag ttcacttaca ccgcctctca
      181 gcccggtacg caccagaaaa tcattgatat ggccatgaat ggcgtcggat gtcgcgccag
      241 tgcacgcatt atgggcgttg gcctcaacac ggttttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acaaccgggc agtgatgtga ttgtctgcgc tgaaatggac
      361 gaacagtggg gctacgtcgg tgctaaatca cgtcagcgct ggctgtttta cgcgtatgac
      421 aggatacgga ggacggttgt ggcgcacgtc ttcggtgaac gcactctggc cacactggag
      481 cgtcttctga gcctgctgtc ggcctttgag gtcgtggtat ggatgacgga tggctggccg
      541 ctgtatgaat cacgcctgaa gggaaagctg cacgttatca gcaagcgtta cactcagcgc
      601 attgagcgac ataatctgaa tctgagacaa catctggcaa ggctgggacg gaagtcactg
      661 tcgttctcaa aatcggtgga gctgcatgac aaggtcatcg ggcattatct gaacataaaa
      721 cactat
//
LOCUS       DAZPEB010000442          723 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZPEB010000442 DAZPEB010000000
VERSION     DAZPEB010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..723
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.448"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..>723
                     /locus_tag="ACHURJ_004403"
     CDS             <1..>723
                     /locus_tag="ACHURJ_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1283054.1"
                     /translation="REKQRLIGVLSKICNSIEYTKIKLTLTLDDLQTKKENLRKLLQE
                     HINMLKKDLDMRLQDAAIDVHRLDSTARKTSEHLRKRIKKTLPLSLFKSIDFKQASDC
                     FTKHKISIKIDKEPYAEGIESIPYINEGDIQADLILKDIQRIILSNLFSTGCSQHTVI
                     EDFNMLIDHIKSSADLAGKLVLVMSKEIFQQYNRMLFDNPNLRELMRKNDDGSMNITT
                     ESGTRKVYFLPFVNQPFSLVVKD"
BASE COUNT          260 a          136 c          120 g          207 t
ORIGIN      
        1 cgtgagaagc aacgactcat tggagtatta tcaaaaattt gtaactccat cgaatacact
       61 aaaatcaagc tgactttaac attggacgat ctccaaacaa aaaaagaaaa cttaagaaag
      121 ttgcttcaag agcatattaa catgctaaag aaagatcttg atatgagact tcaggatgct
      181 gccatagatg tacatcgact agatagcact gcacgtaaaa catcagaaca tcttcgtaaa
      241 cgcatcaaga aaacgctccc tctttcactt ttcaaatcta ttgattttaa gcaggctagc
      301 gattgtttca caaaacataa gatatctatt aagatagata aagaaccgta tgccgaagga
      361 atagaatcca tcccttatat caatgaaggt gatattcaag cagacttaat tctcaaggac
      421 atccagagaa taatactttc aaatttattt agcactggct gttcgcaaca taccgtaata
      481 gaagatttca atatgttgat tgatcacata aaatcatctg ctgacttggc tggcaaactg
      541 gttcttgtga tgagtaaaga gatctttcag caatataatc gtatgctttt cgataatccc
      601 aacctcagag aactaatgag aaaaaatgat gatggctcta tgaacataac tactgaatcc
      661 ggtacccgta aggtttactt cttaccattt gttaatcaac cattttctct tgtggtaaag
      721 gat
//
LOCUS       DAZPEB010000443          719 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZPEB010000443 DAZPEB010000000
VERSION     DAZPEB010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.449"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            14..682
                     /locus_tag="ACHURJ_004404"
     CDS             14..682
                     /locus_tag="ACHURJ_004404"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR1283055.1"
                     /translation="MVKLMKKNTDDGAKIYTPITLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFETYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
BASE COUNT          228 a          143 c          147 g          201 t
ORIGIN      
        1 ataaaactat aacatggtga aattaatgaa aaaaaacaca gatgatgggg ctaaaattta
       61 cacaccaatt accctaaagc tttatgactg gtgggttttg ggagtatcaa atcggcttgc
      121 atggggatgt cctacaaagg aacaccttct tccacacttt ctggaacatt taggtaacaa
      181 ccatctggat attggtgttg gaactgggtt ttaccttact cacgtacctg agagtagtct
      241 gatatcttta atggatttga acgaagctag cctgaacgcg gcatctacaa gggctgggga
      301 atcaaaaatt aaacataaaa ttagccatga tgtttttgaa acttatcccg cggcgttaca
      361 tggtcaattt gattccattt ccatgtttta ccttcttcac tgcctgcctg gaaatatatc
      421 tacaaaaagc tgtgtaatac gcaatgcggc gcaggcctta actgatgatg gaactctata
      481 cggagccaca attcttggcg atggagttgt gcacaatagc ttcggtcaaa aactgatgcg
      541 catttacaat cagaaaggca tcttttcaaa cacaaaagat tccgaagaag gcttaacaca
      601 tatactctca gagcatttcg agaatgttaa aaccaaggtt caaggtactg tagtaatgtt
      661 ttctgcttca gggaaaaaat agcatccaac cgcagcacgt tcttgcttaa gacgtgcta
//
LOCUS       DAZPEB010000444          714 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZPEB010000444 DAZPEB010000000
VERSION     DAZPEB010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.450"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(14..144)
                     /locus_tag="ACHURJ_004405"
                     /pseudo
     CDS             complement(14..144)
                     /locus_tag="ACHURJ_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611212.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            142..225
                     /gene="tnpB"
                     /locus_tag="ACHURJ_004406"
                     /pseudo
     CDS             142..225
                     /gene="tnpB"
                     /locus_tag="ACHURJ_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          194 a          144 c          182 g          194 t
ORIGIN      
        1 gtggcgcttc caatcagtag aatatgtgtt tttcaatgaa ggagccgatg acttttcgtg
       61 gatttccacg gaacgcgaga agcagattgt tttacgtgcc agccgcttga tgcgggtgcg
      121 gagggtcagg ttgttacgtt caagatatag catgtgctgg atgaagatcc cttttctggt
      181 cacctgttta tctttcgtgg ccgacggggt gacactgtta aaatgtaatg aacgcatccc
      241 acacaattaa acacggagta gtatgatggt taaaagggtg aaactggcag gtgtggagcc
      301 tcgcagcttt ccggcatcat actgcccaat acgggatgca taaagcgtcc tcagactgga
      361 gactccacaa atgaaatata caccggttgg cgttgatatg gcaaaacatg tcattcagat
      421 tcacttcatc aatgagcaca tgtcagcgcc agtgatataa gacggtaatg gggatgcgga
      481 cgatttcctg gactctcaga agatgaaaca tgtttactga agaacaaaaa atttgcgcta
      541 ttgagcttta tttcaaatat ggtaagaagc ttgaggtgta ctggcaatag cggacaccac
      601 catttgttct ttttctaagc agtcatctga taatatttta tcctgaaggc tgccggggag
      661 atattcccca gacgagagtt gaggtagcct gagtttaacg gacactcctt cctg
//
LOCUS       DAZPEB010000445          710 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZPEB010000445 DAZPEB010000000
VERSION     DAZPEB010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.451"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..>710)
                     /locus_tag="ACHURJ_004407"
     CDS             complement(<1..>710)
                     /locus_tag="ACHURJ_004407"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFR1283056.1"
                     /translation="ALKKAAFVFFQNEDDRQLLINSGCVNYEKTCRIFGSGVDLSKFL
                     PSNKNLKQRSCVKFILVARLLYTKGILHYLKAAEIIKSKYPNCSFSLLGAFEKNEKII
                     TKELIEGFCTRGIVNYYGTSDDVASIMKHYDAIVLPSFYREGVPKALIEGASSGLAIL
                     TTDNVGCRDMVQNEVNGFLCQPNDLDSLVDIIEKYILLTDEMKNTMSIKSREFAESHC
                     DENKIIEKYTNTINDVLN"
BASE COUNT          234 a          139 c           92 g          245 t
ORIGIN      
        1 atttaaaacg tcattaattg tattagtata cttctctatg atcttatttt catcacaatg
       61 tgattcagca aattctctcg acttaataga cattgtgttt ttcatttcgt ctgtcagtag
      121 aatatacttt tcaataatgt caactaagct atcaagatca ttaggttgac acaaaaaacc
      181 attaacttca ttttgcacca tatccctaca gccaacatta tctgttgtca aaatagctaa
      241 cccactagaa gcaccttcaa tcaacgcttt aggtacacct tctctataaa atgatggtaa
      301 tactattgca tcataatgct tcattatact agcaacgtca tcggaagtcc catagtaatt
      361 aactataccc ctcgtacaaa aaccttcaat caattccttc gtgattatct tttcattttt
      421 ttcaaatgca cctaacagtg aaaatgaaca attaggatat ttggatttta ttatttctgc
      481 ggccttaaga tagtgaagta tgcctttggt atatagaagt ctagcaacaa gtatgaattt
      541 cacacatgat ctttgcttta aattcttatt tgagggaaga aattttgata aatcaacacc
      601 agacccaaat attcggcatg ttttctcata attgacgcag ccagaattaa tcaataactg
      661 acgatcatcc tcgttttgaa aaaaaacgaa tgctgctttc tttaatgcta
//
LOCUS       DAZPEB010000446          705 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZPEB010000446 DAZPEB010000000
VERSION     DAZPEB010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..705
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.452"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(54..125)
                     /locus_tag="ACHURJ_004408"
                     /pseudo
     CDS             complement(54..125)
                     /locus_tag="ACHURJ_004408"
                     /inference="COORDINATES: protein motif:HMM:NF013446.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            185..334
                     /locus_tag="ACHURJ_004409"
                     /pseudo
     CDS             185..334
                     /locus_tag="ACHURJ_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612150.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            361..465
                     /locus_tag="ACHURJ_004410"
                     /pseudo
     CDS             361..465
                     /locus_tag="ACHURJ_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          236 a          143 c          134 g          192 t
ORIGIN      
        1 caaggtcatc gggcattatc tgaacataaa acactatcag taagttggag tcattacctc
       61 ctcccgaact caaagctatc atacagataa ttactggtta accctatata gggcatttta
      121 aagtgttgtg atcttaccca gcaatagtgg acacgcggct aagtgagtaa actctcagtc
      181 agaggtgact cacaagacaa aacagtttca accagtaaaa aaacgtaaac accattcgca
      241 tgaatttcgt agttaagatc tgaagcttgc agaatgcatc ggtgttactg ccggagtccg
      301 tgaacttagt ctgcatgaat caccacagct ttacatctgt cgcagtaaac agcaaaatca
      361 gcaaacttac ttaattatat cgagtgcgat tacaatcgct ggcgatggta cagttggtgt
      421 ggcggtctca gtccggaaca atttgaaaat cagaatctca cttaaaacgg catccatttt
      481 acaaaagatg gccgtctgtg tatttgtaat gaataccttc tacatgttta gagtgcataa
      541 tccacgacaa ggcgatatcc taaaaattag ctagcaggca actttgatgg catagataat
      601 gtttttagta aaaaagagtt gactgaatat aatacagcct cttcctacat aagaaggaat
      661 gtgctcacaa aaaaaaaaca ttagtctgct tcctgttctt acaat
//
LOCUS       DAZPEB010000447          686 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZPEB010000447 DAZPEB010000000
VERSION     DAZPEB010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.453"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            201..389
                     /locus_tag="ACHURJ_004411"
     CDS             201..389
                     /locus_tag="ACHURJ_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFR1283057.1"
                     /translation="MYKITATIEKEGGSPASWTRYSKTKLTKSECEKMLSGEKEAGVS
                     REQKVKLINFNCEKLQSS"
     gene            509..655
                     /gene="xisR"
                     /locus_tag="ACHURJ_004412"
     CDS             509..655
                     /gene="xisR"
                     /locus_tag="ACHURJ_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFR1283058.1"
                     /translation="MVEYGLMLRTGLGARQIEAYRQNCWVEGFHFKRVSPLGKPDSKR
                     GIIW"
BASE COUNT          234 a          127 c          163 g          162 t
ORIGIN      
        1 cccgctcatg ggaaagaatc aaaggtatgg actggccgac aatcaaagct ggtcgtaatg
       61 cactatggat tgaactggaa ggcgttgaat atgcattccc ggatccacaa acacaaaacg
      121 aaacgcaaca agcaggatac gatgagaata ttccggtgta aaaccaagtg cattttggga
      181 aaatattaat ggaggtgttc atgtataaaa ttacagccac tattgaaaag gaaggtggtt
      241 ctcctgccag ctggacaaga tactcaaaga caaaactaac gaaatcagaa tgcgaaaaaa
      301 tgctctcagg ggaaaaagaa gctggcgttt ccagagagca gaaagtaaaa ttgataaatt
      361 ttaattgcga gaaacttcag tcctcgtgaa ttgcattgta ttcaaattaa aacttcatag
      421 ctgattatta ataatcaaca tcgggcgtca atttcagcct aacattggcg cctgccagag
      481 gtgatgcgat ggctttaatg aagagtggat ggttgaatac ggcctgatgc ttcgcactgg
      541 tctgggtgcc agacaaattg aagcataccg ccagaactgt tgggtggaag gcttccactt
      601 caaacgagta tctcctttag ggaagccaga cagtaagcga gggattatct ggtaaaacta
      661 tccgaagata aatcagttta tcaaag
//
LOCUS       DAZPEB010000448          686 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZPEB010000448 DAZPEB010000000
VERSION     DAZPEB010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.454"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..684)
                     /gene="ospC2"
                     /locus_tag="ACHURJ_004413"
     CDS             complement(<1..684)
                     /gene="ospC2"
                     /locus_tag="ACHURJ_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HFR1283059.1"
                     /translation="MNPNKDTKALQKNISCVTNSSSSGISEKHLDHCADTVKSFLRKS
                     IAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSLEHLDKVSRHYLSEIIQKTHPLS
                     SDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFDKIKSENIQTYKNTFSEDIEEIA
                     NHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYIIDHDSPYGYMTLTDHFDNAIPPV
                     FYHEHQSFFL"
BASE COUNT          217 a           95 c          136 g          238 t
ORIGIN      
        1 taagaaaaat gattggtgct catggtaaaa aacaggtgga atagcattat caaagtggtc
       61 ggttaatgtc atatatccat atggagagtc atgatcaatg atatacgcat tagcaccaaa
      121 atccacagta tgatgtgttt tatttagtgg gagtgtttct tgatgattgg aaatttcaac
      181 cccaaaaaaa acaaaatcgt ggttagctat ttcttcaata tcctcagaga atgtgttttt
      241 atatgtttgt atattctcag acttaatttt atcaaaggac ttaatattaa gagtattatt
      301 tgacaggtta gcgttacttt gatgtctgaa gttaaaatca gaattaataa taatagagag
      361 caagtgacgt tcatccgaag aaagaggatg agttttttgt attatttcag aaagatagtg
      421 tctggacacc ttatcgaggt gctcaagtga ccgaaatgat gaccgtgctc cagatggcgc
      481 ttcaatagaa agatttaatg atttgaaact ggtcccttga gagaacattt tactataact
      541 ttgcgctgct atagacttcc ttaaaaagct tttcacagta tcagcacagt gatctaaatg
      601 tttttcactt atgccagaag aggatgagtt tgttacgcat gatatgtttt tttgtaatgc
      661 ttttgtatct ttgttcggat ttattt
//
LOCUS       DAZPEB010000449          664 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZPEB010000449 DAZPEB010000000
VERSION     DAZPEB010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.455"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
BASE COUNT          210 a          135 c          105 g          214 t
ORIGIN      
        1 tatgttacca agaacatatc ttttattata atatttattg aacttttgta aaaataaaaa
       61 aatacctcat tgtgcgaata aagtaacggg ggccatagat ttctctacct accttttggt
      121 gttcatacct gaggctgata gtttcaggat tccgcgacaa ttctactcac cttcttgttc
      181 tctcgccaat atgaaatcaa tcagaaaagc gttccataag aacaacgata agacaataaa
      241 ttgtcatcac ggctgcaata gccaacgtgc ctttcagtac caacatgaca acctcctgta
      301 tttgacgtaa cgtgtgctga taaatatgta tttgtatctc aactgactca atacaacact
      361 taagtatagt ttttcagatt ttgtttttcc cgtgaaatat tatggaaatg gcagaagcaa
      421 tacattgaat tccgattcga gtgaatacgg ctagcgggca cctcacttta gcactgaagc
      481 catcaacttt tatttgactt atttcaatgg aaagctttct cactatcagt acaggtaatt
      541 cccatccttt taaagtcgag agtcagactg cgactaatac agttaaagtg accaaactca
      601 gttatcctct acacatttta cctcatgttt acttggacaa ttcattggtg cttttataaa
      661 agaa
//
LOCUS       DAZPEB010000450          658 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZPEB010000450 DAZPEB010000000
VERSION     DAZPEB010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.456"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            277..576
                     /locus_tag="ACHURJ_004414"
     CDS             277..576
                     /locus_tag="ACHURJ_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFR1283060.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMEFGERTVIKNPEYFSTYMQEELKALV"
BASE COUNT          211 a          128 c          131 g          188 t
ORIGIN      
        1 ctaactatat aaataaacat aaccatgccg ttgtgttcgc ccttcgatta gcacctctgg
       61 ttttctttct tagctgaaca atctggttat agcgtgcgga ctgaccgtta attttctcca
      121 tcttactgga taacacacca tgagctgcta cctttaaaac acctgaatgt caacaggtta
      181 actcgtgcca tatcgttata atccctttga tattagatgc aaattaaggt catatatagc
      241 cttatcgata acatggttaa ttttaaggat aagaatatgc ctgctgtaat agataaagcc
      301 ctggatttca ttggtgccat ggatgtatca gcgccaacac caagttcgat gaatgaaagc
      361 acagcgaagg gaatctttaa atatttaaaa gaactgggag tacccgcgag tgccgctgat
      421 attacggcgc gagctgacca ggaaggctgg aaccctgggt tcacggaaaa aatggttgga
      481 tgggcaaaaa aaatggagtt tggtgaacgc actgtaatta aaaatcctga atacttttca
      541 acatatatgc aggaagaact aaaagcactg gtctgagttt aatttatatc agcataaatg
      601 ggtcaggacg cttttaatca catataaaaa gcgtcctttt tctcacaatc aaataatc
//
LOCUS       DAZPEB010000451          646 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZPEB010000451 DAZPEB010000000
VERSION     DAZPEB010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.457"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..239
                     /gene="rclC"
                     /locus_tag="ACHURJ_004415"
     CDS             <1..239
                     /gene="rclC"
                     /locus_tag="ACHURJ_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFR1283061.1"
                     /translation="MAFTTPLVTLSFLITTPEAWVSALGDAHYGFPYLSGAGRLVLKD
                     TLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(399..>646)
                     /locus_tag="ACHURJ_004416"
     CDS             complement(399..>646)
                     /locus_tag="ACHURJ_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFR1283062.1"
                     /translation="SWQHYPISQVILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLT
                     EKEMAQISSLDLGYVGESVKHFNPEFVRGCLAVKIHD"
BASE COUNT          186 a          144 c          127 g          189 t
ORIGIN      
        1 tgatggcatt taccacgccg ctggtaacac tctcattttt aatcaccacc ccggaggcat
       61 gggtatccgc attgggtgac gctcattatg gtttccctta tttatccggt gctggtcgct
      121 tggtattgaa agatactctg atgctggcag gtgctgtcat gataatggca gattcggcgc
      181 gggaaattct taaacaacgc agtaatgaat ccagttcaac gttaaaaact gaatattgat
      241 aatccgaaca ctgtctgttg ttcacttttt caggcgtcag gccccctttc tgatgatgaa
      301 gactgacgcc atattaccgg agatatgaca gagtgaagat caatatcact cccctgttaa
      361 aagattcttg tcgtctcagg taaaatatat gattaatatc aatcatgtat ttttacagca
      421 agacaaccac gaacaaattc aggattaaaa tgttttaccg actccccaac ataacccaaa
      481 tcaagcgaac taatttgtgc catttctttc tctgtcagtg agaaatccca gatagcaaaa
      541 ttttcttcga tacgctgttg atgtgtcgat ttcggaataa cgaccactcc ccgttgaata
      601 ttccaacgca gaatgacttg agaaatcggg taatgctgcc aactta
//
LOCUS       DAZPEB010000452          631 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZPEB010000452 DAZPEB010000000
VERSION     DAZPEB010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.458"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            106..>631
                     /locus_tag="ACHURJ_004417"
     CDS             106..>631
                     /locus_tag="ACHURJ_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HFR1283063.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEK
                     MAAIHKALDECSAEH"
BASE COUNT          162 a          170 c          167 g          132 t
ORIGIN      
        1 cctaaatagc tgcgcggaat agtagatccc tttgagggaa cttagcccgg attgtgcgat
       61 ctgatcaatc gccaaatcaa aacaaatcac caaccggact gagcaatgcc gatcatagca
      121 ccaatttccc gtgacgaacg acgcctgatg cagaaagcca tccataaaac acacgataaa
      181 aattatgccc gcagactgac tgccatgctg atgctgcacc ggggcgaccg tgtcagcgac
      241 gttgccagaa cgctctgctg cgcccgttcc tctgttggac gctggattaa ctggttcacg
      301 cagtcgggtg ttgagggact gaaatcatta cctgccgggc gtgcccgtcg ctggccgttt
      361 gagcatatct gcacactgtt acgtgagctg gtaaaacatt ctcccggcga ctttggctac
      421 cagcgttcac gctggagtac agaactgctg gcaataaaaa tcaatgagat aaccggttgc
      481 cagttaaatg ccggaaccgt tcgccgctgg ttgccgtctg cggggattgt gtggcgaagg
      541 gctgcgccaa ctctgcgtat ccgtgacccg cataaagatg aaaagatggc agcaatccat
      601 aaagcactgg acgaatgcag cgcagagcat c
//
LOCUS       DAZPEB010000453          626 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZPEB010000453 DAZPEB010000000
VERSION     DAZPEB010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..626
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.459"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            258..398
                     /locus_tag="ACHURJ_004418"
     CDS             258..398
                     /locus_tag="ACHURJ_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR1283064.1"
                     /translation="MSQKSLTAITFCVTAILIIWMLHGSLCEIRMSFWGAEFAAFLQC
                     KQ"
BASE COUNT          174 a          159 c          166 g          127 t
ORIGIN      
        1 gtggcatgaa ctgccaactt cacacagagg aaacaatcag atgagtttgc ccttacctca
       61 gaattaacga gcagaattgc cggagtaaca aaaaagcgat agattacgcg cgggtgcctt
      121 tcggctgatg gtcggaggga atacctgaag gccagatgtg gaaaggcccc ggaaaacatt
      181 tttgtttaac cgaggcccta accgtctacc ctaagcaagt gataggttag cgcctccccg
      241 aaaaaggagc aagcgctatg tcgcaaaaat cgcttacggc catcacgttc tgcgtgacgg
      301 caatcctcat catctggatg ctgcacggtt cgctgtgcga aatacggatg agcttctggg
      361 gagcggagtt tgcggcgttc ttacagtgta agcagtaagg aaaccgcgac gggggagcaa
      421 tcccccgtca atcggttgcc agggtaaggt cgataaggca ccctatctca cagacatgaa
      481 caacaaaccc gcagcgtaaa aactgcgggg gttctttttg gtgtcctcac cgacttgaaa
      541 cagtgatcag actataagac aaagcccaca atgtagcggg cttactcccc tccccccact
      601 aaatgtggac gcatcgtgga acgaag
//
LOCUS       DAZPEB010000454          625 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZPEB010000454 DAZPEB010000000
VERSION     DAZPEB010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.460"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..247)
                     /locus_tag="ACHURJ_004419"
     CDS             complement(<1..247)
                     /locus_tag="ACHURJ_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1283065.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPEIPIENNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPGGKCIITGDFQKELAYLQNVILYN"
     gene            complement(269..>625)
                     /locus_tag="ACHURJ_004420"
     CDS             complement(269..>625)
                     /locus_tag="ACHURJ_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1283066.1"
                     /translation="AITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDNYIIQTL
                     DVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTQKEAILTAPTHFNKTCITSDC
                     WQILFFSPDHFNNDFY"
BASE COUNT          197 a           97 c          115 g          216 t
ORIGIN      
        1 cattataaag aattacattt tgtaagtacg ctaattcttt ttgaaaatca ccagtgataa
       61 tacattttcc acctggttgt aacgcgttaa gtaaagtgac catttgaata gaggtctgtt
      121 ttagtttttc tatacctggt gctagtgctt cgagattatt ttctatgggg atttcaggaa
      181 ggtcattcat ttttcgacgg actaaatcac tactaatatc attaccaatt gatgaaacag
      241 ggaacattat tttatccttt ataaataatc aataaaaatc attattaaaa tgatcgggtg
      301 aaaaaaagag aatttgccaa caatctgaag taatgcaggt tttattaaag tgcgtagggg
      361 ctgttaagat ggcttctttt tgggtgaact ttgcgcaata cttagaaatc gtctccttaa
      421 tacattccag ctctttttta ttgacggctg agcaagtaca ttgttcacca aaaggtgcaa
      481 aagtgcctac atctaatgtt tgtatgatat aattatcatg ttctcctgta gaaatatcta
      541 tgatgaatgg actcatattt tccggttgga ttcggatcat gacagaagga acatcgcctt
      601 gatagaaatt caatgcagta attgc
//
LOCUS       DAZPEB010000455          620 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZPEB010000455 DAZPEB010000000
VERSION     DAZPEB010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.461"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..532
                     /gene="ospB"
                     /locus_tag="ACHURJ_004421"
     CDS             <1..532
                     /gene="ospB"
                     /locus_tag="ACHURJ_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HFR1283067.1"
                     /translation="NAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGS
                     ELIDVQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFK
                     EKESLLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTV
                     KRSSQKKTFIINKELD"
BASE COUNT          204 a          100 c          110 g          206 t
ORIGIN      
        1 gaatgctgct gcttattttt ctgtaaaaaa agcgaaaata aaaacagtaa acgatactga
       61 ttttaaagag tataataagg tttatattct tgggcacggt agtcctggtt ctcatcaatt
      121 aggccttggt tcggaactta ttgatgtaca aacaatcatt tcaagaatga aagactgtgg
      181 tattctaaat gtgaaagata tccgttttac ttcatgcggc tccgctgata aagtggctcc
      241 taaaaatttt aacaatgccc ctgctgaaag tctttcttgt atccttaact ctctgccttt
      301 ttttaaggaa aaagaatctt tgctagagca gataaaaaaa caccttgaaa acgatgagtc
      361 attgagtgat ggtctaaaaa tatccggcta tcatggatat ggagttcact atggtcaaga
      421 gctttttccc tactcacatt atcgttcaac ttcaattcct gctgatccgg agcatacagt
      481 aaaaagaagc tctcagaaaa agacttttat tattaataaa gaactggatt agtataaaat
      541 ttttaaccta tagttaattg tttgcattta agatgaagtt atttttttct ttagtagaaa
      601 agtgcttccc tatgagggaa
//
LOCUS       DAZPEB010000456          615 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZPEB010000456 DAZPEB010000000
VERSION     DAZPEB010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.462"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
BASE COUNT          194 a          121 c          107 g          193 t
ORIGIN      
        1 actgttacga caacgctccg atggaaatct tctggggaac gctgaaaaat gaaagcctga
       61 gccactatcg ttttaagagc cgggatattt cgtcagcata cgggaaaact gattgaggtc
      121 ggcctcagtt ttgaggcctt tagccagttc agccgcaagg gctttaagct tcttttcgtc
      181 cataatttac ctgtctccgt tgttggagta aacatatcaa aaacaggcaa ttacacaatt
      241 taaattacag tctcaaaaca aacccaacgg tcaatatagt ttaccagcaa gcttactagc
      301 aaagtgctaa tcatttggta tctttcgtgc tattctcctt ttacgtaagt acttaagggg
      361 aacgtattta ctacttttaa agaaagctcc gttactatct gaaatgcaat gagataataa
      421 acgattactc tcggttagta tctcatgatc atttacagac tttgcataat cgcgaatact
      481 atataacaga gttttaacat tacaggatga atagtctctc ttatcaactc ctaataaaag
      541 aaggattatg gtcagtcgtt tttgctgtct tatcatatat tatttagttc agttcctaac
      601 aaaaacataa caatt
//
LOCUS       DAZPEB010000457          592 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZPEB010000457 DAZPEB010000000
VERSION     DAZPEB010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.463"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            244..>592
                     /locus_tag="ACHURJ_004422"
     CDS             244..>592
                     /locus_tag="ACHURJ_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1283068.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNND"
BASE COUNT          171 a          111 c          122 g          188 t
ORIGIN      
        1 atcgccatac cctaaccaat tgcacttgtg aatttatttt tgcttaattt tgaattaaga
       61 aaatttaatt tgttcaaaag cccttaaagt gcacaatagg cgtcctaatg aacaatggtt
      121 gtaaattttg gtaacaaata tcattgctca ttaatatcta gcatctgctt ataacatttg
      181 tgtcatgaat agtcattctt caaaccagta gtctgactaa tggaatttta ttcaaggatt
      241 tttatgtcta aaaaattagg ttttgccctg tctggtctta tgctggcaat ggttgcaggt
      301 actgcttctg ctgatatgga tggtggtcag ttaaatatca gtggtctggt tgttgataac
      361 acctgtgaaa cccgcgttga cggcggtaat aaagatggtc tgatcctgct gcaaactgca
      421 actgttggtg aaatcgatgc tggcgtgctg aatgacaccg ttggcgctaa agccaaacct
      481 ttcagcatca ccgttgactg cagcaaagca aatccgaatc caggtagtac agctaaaatg
      541 accttcggtt ctgttttctt tggtaatagc aaaggaacgc tgaacaacga ca
//
LOCUS       DAZPEB010000458          575 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZPEB010000458 DAZPEB010000000
VERSION     DAZPEB010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.464"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            185..>575
                     /gene="bluF"
                     /locus_tag="ACHURJ_004423"
     CDS             185..>575
                     /gene="bluF"
                     /locus_tag="ACHURJ_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HFR1283069.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFR"
BASE COUNT          169 a          116 c          115 g          175 t
ORIGIN      
        1 atgataaaac atattgcatc atttaactga caatctatac gagatttttc gccaactagt
       61 gttcaacttt ttccattttt tatcaatgta aagaaacggt tatgttttgg tattaaaaga
      121 aagacacaag aagatatgtc tgttaccgtc ttactctcgc ctcacccatt accctggatt
      181 gttaatgctt accaccctta tttatcgtag ccatatacgt gacgacgaac ctgtcaaaaa
      241 aatcgaagaa atggtttcga tagcaaatcg caggaacatg cagtctgacg taacagggat
      301 cttactgttt aatggttctc attttttcca gcttctggaa ggtccggaag aacaggttaa
      361 aatgatatat cgggctatat gccaggatcc acggcactat aatattgttg agctgctgtg
      421 cgattacgcg cctgctcgcc gttttggcaa agcgggaatg gaattatttg atttgcgcct
      481 gcacgagcga gatgacgttt tacaggccgt attcgacaaa ggcacatcaa aatttcagct
      541 aacttatgat gacagagcgc tacaattttt tcgta
//
LOCUS       DAZPEB010000459          571 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZPEB010000459 DAZPEB010000000
VERSION     DAZPEB010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.465"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(125..523)
                     /locus_tag="ACHURJ_004424"
                     /pseudo
     CDS             complement(125..523)
                     /locus_tag="ACHURJ_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612066.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
BASE COUNT          128 a          146 c          132 g          165 t
ORIGIN      
        1 aaatgaagtt tactgtaaaa aaacgatact tttttcgtaa gcgatgagat gtggagccag
       61 aaaaatggct gcgttacgtc attgaactgt ccttgaccca cgatattcag acagtcgttg
      121 tatcttaagt tgacattacc cgctgacgtc gatattccct tggtgagaga taacccaacg
      181 cactatgcgg atggttttca ttgtaatgct cgatcgccac tgcaaggtta tgcaatgccg
      241 ttcttggttt cggcatgaac gcgatgcagt cttccttcat cgttttcacg aaccgttctg
      301 ctattccatt actctgaggg ctactgactg ccgtcgtgca gggctccaga ttcaactctc
      361 tggcgaactg ccgtgtttca tgagccgtat aagccgaacc gttatcggcc actggattgg
      421 ccgttccggt cctttatcgc catatcgctg acatgacatc ctgcaccgtt gctttgtcat
      481 aatctcccgt gcctgctgcc cagtctatgg cttcacgatc gcaatatgga acgtaacgct
      541 gtcatcggga gaatcgtcaa tgcggcattc g
//
LOCUS       DAZPEB010000460          559 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZPEB010000460 DAZPEB010000000
VERSION     DAZPEB010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.466"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            69..404
                     /locus_tag="ACHURJ_004425"
     CDS             69..404
                     /locus_tag="ACHURJ_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFR1283070.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            475..>559
                     /locus_tag="ACHURJ_004426"
     CDS             475..>559
                     /locus_tag="ACHURJ_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1283071.1"
                     /translation="MPLHGAGCLPVNSVPAPESAIIPYTYSL"
BASE COUNT          191 a          122 c           96 g          150 t
ORIGIN      
        1 gtttacattc aaaaatatca tttatacttt tcagaacatc accagcaagg cataaacaag
       61 gaaactaaat gaagtggatt gtgattgata cagttatcca gccatcatgc ggaatatctt
      121 tttcagtcat atggagtaaa ataaaattaa taatctggta tcaatcggat gctttcttac
      181 ctcctgaaag tatatttaca ctgactcaca caggcatcat gctcaataac aaagtgctac
      241 ctgtaaccat ttacaacgta gtaccattca ataaaacatt ctggaattta atcaaaaaca
      301 gccaggaatg ccctacaaat acagataacg tattgaatga atgctttaat aaccgttgca
      361 ctctgcaaat atgtccttat ggactaaaac aacaaagtcc ataaggagtt tactcacatc
      421 tgacaaaatc aatataaaca gcccctccgg agaggggctg gagagtggcg ctatgtgcca
      481 ttgcatggtg ccgggtgcct cccggtgaat tcagtaccag cacctgaatc cgcgattatc
      541 ccatatacct actcgctga
//
LOCUS       DAZPEB010000461          558 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZPEB010000461 DAZPEB010000000
VERSION     DAZPEB010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.467"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            2..>558
                     /gene="fimZ"
                     /locus_tag="ACHURJ_004427"
     CDS             2..>558
                     /gene="fimZ"
                     /locus_tag="ACHURJ_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HFR1283072.1"
                     /translation="MIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDYLRTRP
                     VDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANGFVSKC
                     NDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRYLVSGL
                     SNKEIADKLLLSNKTVSAHKSNIYG"
BASE COUNT          180 a          102 c          106 g          170 t
ORIGIN      
        1 ggtgatcatt atggatactc atcctatcat cagaatgtct attgaagttc tgttgcaaaa
       61 aaacagtgaa ttgcagattg tcctgaaaac ggatgattac cgcataacca tcgattatct
      121 ccgaacccgt cctgttgatt taatcattat ggatatagac ttgcccggaa cagacggttt
      181 taccttcctg aaaaggatca aacaaatcca gagcacagtg aaagtgttat ttttatcatc
      241 gaaatcagaa tgcttttatg ctggcagagc gatacaagct ggtgctaacg gttttgtcag
      301 taaatgcaat gatcagaatg atatttttca tgccgttcag atgatcctct ccggatacac
      361 gttttttccc agcgaaacgc ttaactatat aaaaagcaat aaatgtagta cgaatagttc
      421 aacggtcact gtgctatcta atcgtgaagt gaccatatta cgttatctgg ttagcggatt
      481 atctaataaa gaaattgccg ataagttatt gcttagcaat aaaacagtta gtgcgcataa
      541 atctaatatt tatggcaa
//
LOCUS       DAZPEB010000462          556 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZPEB010000462 DAZPEB010000000
VERSION     DAZPEB010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.468"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..>556)
                     /locus_tag="ACHURJ_004428"
     CDS             complement(<1..>556)
                     /locus_tag="ACHURJ_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1283073.1"
                     /translation="AFLTGIAGALQTPTLSIFLADELKARPIMVGFFFTGSAIMGILV
                     SQFLARHSDKQGDRKLLILLCCLFGVLACTLFAWNRNYFILLSTGVLLSSFASTANPQ
                     MFALAREHADRTGRETVMFSTFLRAQISLAWVIGPPLAYELAMGFSFKVMYLTAAIAF
                     VVCGLIVWLFLPSIQRNIPVVTQPV"
BASE COUNT          171 a          128 c          142 g          115 t
ORIGIN      
        1 tacgggttgg gtaacgacag gaatatttct ttgtattgat ggcaaaaaca accagactat
       61 cagcccgcaa acaacaaatg cgatggcagc ggtgagatac atcactttaa aactaaaccc
      121 cattgccagt tcataagcga gcggtggccc gataacccag gcaagcgaga tctgagcacg
      181 taaaaatgta ctgaacatga ccgtctcacg gcctgttctg tcggcgtgtt cacgggcgag
      241 ggcgaacatt tgcgggtttg cggtagaagc aaaactactc agaagtacgc ccgtagagag
      301 gagaatgaag tagttgcgat tccacgcaaa aagcgtgcag gccagcactc caaataagca
      361 acatagcaga atcagtaatt tacggtcgcc ttgtttatcg gagtgccttg ccagaaattg
      421 actgaccaga attcccataa tagcgctacc ggtgaagaaa aaacctacca ttataggacg
      481 ggctttcagt tcatctgcga ggaatatact tagggtagga gtctgaagag cgcccgcaat
      541 acccgtcaga aaggcg
//
LOCUS       DAZPEB010000463          554 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZPEB010000463 DAZPEB010000000
VERSION     DAZPEB010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.469"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..206)
                     /locus_tag="ACHURJ_004429"
     CDS             complement(<1..206)
                     /locus_tag="ACHURJ_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001448609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1283074.1"
                     /translation="MLAISSNLSKMIIFIFAIIIIVVLCVITYLYLYKDESLVSKHYI
                     NYMAIPENDGVFTWLPDFFFARSGG"
     gene            complement(187..554)
                     /locus_tag="ACHURJ_004430"
                     /pseudo
     CDS             complement(187..554)
                     /locus_tag="ACHURJ_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT          182 a          112 c           74 g          186 t
ORIGIN      
        1 ccaccgctac gtgcgaaaaa aaaatctggg agccatgtaa aaactccatc attttctggt
       61 attgccatat agtttatgta atgtttactt acaagagatt catctttgta taaataaaga
      121 taagtaatta cgcataaaac aacaatgatt ataatagcaa aaataaatat tatcatcttt
      181 gatagattac ttgagatagc cagcatcttg taaagccttt atcgtttttt tatgctctgg
      241 attaatataa tcactacatc tatctgagca atctgttgtt gatggacatg tcaacccatg
      301 gtcatttaca gccaagtctt ttttattatc ttcaatcatt tcagaatggg atagctttac
      361 ttttctgccg tacataccga acagatttaa tccgtaatct ctaatctctt tttcataacc
      421 cagccctttc gccgatcggc taacaccagc gtcatttaat ttagacactc gacaaaatgc
      481 catacaatgg aaaaattgat cagtcccacc taaatttaat ctcttcataa gtatcagata
      541 atatatccca tgct
//
LOCUS       DAZPEB010000464          547 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZPEB010000464 DAZPEB010000000
VERSION     DAZPEB010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.470"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            34..207
                     /gene="ymgI"
                     /locus_tag="ACHURJ_004431"
     CDS             34..207
                     /gene="ymgI"
                     /locus_tag="ACHURJ_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HFR1283075.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVRATYLIPDI"
     gene            209..>547
                     /locus_tag="ACHURJ_004432"
     CDS             209..>547
                     /locus_tag="ACHURJ_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFR1283076.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKM"
BASE COUNT          152 a          105 c          139 g          151 t
ORIGIN      
        1 atgacacttc ttgaataaaa atattgctaa tcaatgagtt attcctcatt taagattatt
       61 ctcattcacg taaaaaacat aattcctatc atcacagcca cgctcatttt gaattatctg
      121 aataatagcg agcgttcttt agttaagcag atcttaattg aagacgaaat tattgtgcgt
      181 gcaacttact taataccgga catctaagtt gaagaaaaaa atacttgcct tcgggcttat
      241 ctcagcattg ttctgctcca cgccagcgat ggcggatatg aatcgcacca cgaaaggtgc
      301 attgctaggc gcaggcgttg gtttacttac tggcaatggt gttaatggcg tactgaaagg
      361 tgcagctgtt ggcgctggtg ttggtgcagt aacagaaaaa ggccgcgacg gtaaaaatgc
      421 gcgtaaaggt gcaaaagtag gtgccgccgt tggtgctgtc actggcgttc tgaccggcaa
      481 tggcctcgaa ggggcgatta aaggtgccgt gattggcggt acgggtggtg caattctggg
      541 taaaatg
//
LOCUS       DAZPEB010000465          547 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZPEB010000465 DAZPEB010000000
VERSION     DAZPEB010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.471"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..496
                     /locus_tag="ACHURJ_004433"
     CDS             <1..496
                     /locus_tag="ACHURJ_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFR1283077.1"
                     /translation="SVDYNIEGFGFVGAYSKSDRTNEQAGDGYGDNAEVWSLAAKYDA
                     NNIYAAMMYGETRNMTVLANDHFANKTQNFEAVVQYQFDFGLRPSLGYVYSKGKDLYA
                     RDGHKGVDADRVNYIEVGTWYYFNKNMNVYTAYKFNLLDKDDAAITDAATDDQFAVGI
                     IYQF"
BASE COUNT          164 a          111 c          121 g          151 t
ORIGIN      
        1 gtctgtagac tacaacatcg aaggttttgg ttttgtaggt gcatatagca aatctgatcg
       61 tactaatgaa caagctggtg acggctacgg tgataacgct gaagtgtggt cattagcagc
      121 caagtatgat gcaaataata tctatgcagc aatgatgtac ggtgaaaccc gcaacatgac
      181 cgttttggct aatgatcatt ttgcaaataa aacccaaaac tttgaagctg ttgtacagta
      241 tcagttcgac ttcggtttac gtccgtcttt aggctacgta tattccaaag gcaaagatct
      301 ttatgctcgt gatggacata aaggtgttga tgctgaccgc gtaaattata tcgaagttgg
      361 tacctggtac tacttcaata agaacatgaa cgtctacaca gcatacaaat ttaacctgct
      421 ggataaagac gatgcagcga ttaccgatgc cgcaactgat gaccagtttg cagttggtat
      481 catctaccag ttctaatcag ccaacgcgcc gcggcgtgtt actgtaaaac ataacactag
      541 cctgagt
//
LOCUS       DAZPEB010000466          544 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZPEB010000466 DAZPEB010000000
VERSION     DAZPEB010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.472"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..>544
                     /locus_tag="ACHURJ_004434"
     CDS             <1..>544
                     /locus_tag="ACHURJ_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1283078.1"
                     /translation="DSFLRLDFLGYNAQIIQRSDNTCELTINEPLKNQEISTGNINAN
                     CPLKDIYNEIRRLNATFSCGTGDIVDLSSLDLRNVDLDYYDFTDKHMANTILNPFKLD
                     STNFTNANMFQVNFVSSTQNATISWDYLLKITPVLISISDMYSEEKIKFVESCLNELG
                     DITEEQLKIMRFAIIKSIPRA"
BASE COUNT          193 a           88 c           87 g          176 t
ORIGIN      
        1 gactcgtttc ttcgtctgga ttttttaggt tataacgccc aaattattca acgatcggac
       61 aatacctgcg aacttaccat taatgaaccg ttaaaaaacc aggaaatatc tacaggcaat
      121 atcaatgcta attgtccatt aaaagatatt tacaatgaaa tcaggaggtt gaacgcaacc
      181 tttagttgtg ggactggaga tatagttgat ctatcctctc tggacttacg taatgtcgat
      241 ttagattatt atgatttcac agataaacat atggctaata ctattctaaa tccttttaaa
      301 ttggattcaa caaattttac taatgccaac atgtttcagg ttaattttgt tagttcaaca
      361 caaaacgcca caatctcctg ggattattta ctaaaaataa cgcctgtttt aataagtatt
      421 agcgatatgt attctgaaga aaaaatcaag tttgtcgaaa gttgtttaaa tgagcttgga
      481 gacattaccg aagaacaatt aaaaattatg agatttgcaa ttataaaatc tataccaagg
      541 gcaa
//
LOCUS       DAZPEB010000467          538 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZPEB010000467 DAZPEB010000000
VERSION     DAZPEB010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.473"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(106..270)
                     /locus_tag="ACHURJ_004435"
                     /pseudo
     CDS             complement(106..270)
                     /locus_tag="ACHURJ_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135853.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
     gene            complement(257..>538)
                     /locus_tag="ACHURJ_004436"
     CDS             complement(257..>538)
                     /locus_tag="ACHURJ_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR1283079.1"
                     /translation="TAPGYWFYRNDEFVFDYRLKAEDERDALLAQVSARTGEWEEDLL
                     LGLISDEDKEKLKACRIYAKSLQAMDFSTITDKATYNAINWPERPDAAA"
BASE COUNT          121 a          143 c          132 g          142 t
ORIGIN      
        1 ctcgtctggg gaatatctcc ccggcagcct tcagggaaaa atatcatcag atgactgctc
       61 aaaaaaagaa caaatggtag tgtccgctat tgccagtaca cctcacacac ccggcagaac
      121 ggggaagtca tattccccgt tctgtgtggt gacatggctg aaaacacttt tcagaatccc
      181 tgcacggttg tgtatggagg ttagtgtaat ggttgcgccg tccagcggtt ctcccgtggg
      241 cgttttaatc acacctttaa gctgcggcat cgggacgctc cggccagttg atggcgttat
      301 atgtggcttt atctgtgatg gtgctgaaat ccatcgcctg cagcgatttc gcgtaaatac
      361 gacaggcttt cagcttttct ttatcttcgt cgctgattaa ccccagcagc aggtcttctt
      421 cccattcccc tgtccgggca ctgacctgag ccagaagggc atcacgctca tcttccgctt
      481 tgagtctgta gtcaaagaca aattcatcat tgcggtaaaa ccagtaaccc ggcgcggt
//
LOCUS       DAZPEB010000468          527 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZPEB010000468 DAZPEB010000000
VERSION     DAZPEB010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.474"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..>527
                     /locus_tag="ACHURJ_004437"
     CDS             <1..>527
                     /locus_tag="ACHURJ_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1283080.1"
                     /translation="TILSGQLTIIGIVYPLVIGLVSVLFQKKADRKIAQTAYQRYSGF
                     MLAGLSGLFLSGFILLSVLIKTVFGSYLYGIACLISILWLLINIVLSIWFFIVSLEIL
                     DDVKRQIIIKRYIAFEIVMPHICNKISAKLRLYPIYQKHNYSNLEITQADYKGEYISV
                     ASSYSKEDELSLYHR"
BASE COUNT          168 a           91 c           89 g          179 t
ORIGIN      
        1 aaccatttta agtggccaat tgacaattat tggtattgta tacccccttg ttataggttt
       61 agtaagtgtc ttattccaaa aaaaggcaga tagaaaaata gcccaaactg cttatcagcg
      121 ttactcagga ttcatgcttg ctggactcag cgggcttttc ttatcaggat ttatacttct
      181 cagtgtacta attaaaactg tctttggaag ttatctttat ggtatagctt gtctaatcag
      241 catattatgg cttttgataa acatagttct ttctatttgg ttctttatcg tgagtcttga
      301 aattcttgat gatgtaaaac ggcaaataat tataaagcga tacattgcct tcgaaatagt
      361 aatgccccat atttgtaaca aaatctcagc caagcttagg ttatacccta tctatcaaaa
      421 acataactat tcaaatttag aaatcacgca agccgattac aaaggagaat acatatctgt
      481 ggctagcagt tattctaagg aagatgagtt aagtttatat caccgtc
//
LOCUS       DAZPEB010000469          525 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZPEB010000469 DAZPEB010000000
VERSION     DAZPEB010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.475"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            142..478
                     /locus_tag="ACHURJ_004438"
                     /pseudo
     CDS             142..478
                     /locus_tag="ACHURJ_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          176 a           89 c          109 g          151 t
ORIGIN      
        1 aattatcata tatggttctt ttcatgttga tttcgtcaga aacagaaaat gctgataatg
       61 aattgtgaat aatcaaactg acctctaatt atcctgtatg gagtagtgct tattttcgac
      121 tcaatttcta ctgattagat tatggttatc agtacagtgg gttatgaaaa acggactgga
      181 tctgccagca gtgcttctat tattattcaa aaagaacttc agccggacaa tgctataatc
      241 cagggaaagg ctataagctc tgttggacgg aatgtggaaa tcagcactgg taaaaagggg
      301 aactgacatt aacacaagaa ggattcctgc aagcaactgc ttatctgtcc ggaacgcaga
      361 attaaagcat gaccacacag gagccagaat actgtggtcg tacgcattca gtattgtgac
      421 aactaataga aataaaaatc tggccggtgc gaatattaaa cgatgcgata ccgtatagtg
      481 taagtcaaaa gtaacggggt atttaaacca tcttttcata tttat
//
LOCUS       DAZPEB010000470          515 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZPEB010000470 DAZPEB010000000
VERSION     DAZPEB010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.476"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            235..515
                     /locus_tag="ACHURJ_004439"
                     /pseudo
     CDS             235..515
                     /locus_tag="ACHURJ_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT          132 a          136 c          126 g          121 t
ORIGIN      
        1 tccccgccag cccggctaag atggaggcac ggcacagccc gcacacgacg ctgaggctac
       61 agagctgaaa acccacaagg ccatgccccg catggccggg taaagcggag ccggcggcga
      121 cttcctttgt gctctgtccg ggaccggcag gaaaaaggcg gggataatga gatggtcacc
      181 cccaccctaa cagcagtacg ctgttaaggt gaatccctta acggagagac catcatacag
      241 aacatcacac ttattggtat cgatctcgga aagcattcct tccacattca ttgtcaagac
      301 aagtctggta aggctcttct gcgtaaaaaa tttacccagt actaaactga tgcagtttct
      361 ggcaacatgt ccgccctcag ttgttgtaat ggaagcctgt gcgggtgcac actttttggc
      421 tcgccgtatc tctgaatttt ggacatgaaa ctaagctgat ttctccacag ttcgttcgcc
      481 cgtttgtcaa aagtaataag aatgattttg ttgat
//
LOCUS       DAZPEB010000471          515 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZPEB010000471 DAZPEB010000000
VERSION     DAZPEB010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.477"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..153)
                     /locus_tag="ACHURJ_004440"
     CDS             complement(<1..153)
                     /locus_tag="ACHURJ_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR1283081.1"
                     /translation="MLGAIPFHRPTKWVQYISAAPALSQQAVDQEWSYMDFLEHLLHE
                     EKLARHQ"
     gene            152..515
                     /locus_tag="ACHURJ_004441"
                     /pseudo
     CDS             152..515
                     /locus_tag="ACHURJ_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          133 a          114 c          129 g          139 t
ORIGIN      
        1 ttgatgacgt gccagttttt cttcatgaag cagatgctcc aggaagtcca tataactcca
       61 ttcctggtct actgcctgtt gtgacagcgc aggcgctgcg cttatgtact gcacccattt
      121 tgttggacga tgaaatggaa tagcccctaa tatgtcaaag ccaaaatacc cttttgaaaa
      181 gcgccttgaa gtcgtgaatc actacttcac tactgatgat ggttacagga tcatctcggc
      241 acgttttggt gtccccgaac ccaggtcagg acatgggttg ccctctatga aaaacatgga
      301 gaaaaaggct taattcccaa acctaaaggt gttagtgctg atccagagtt gcgtattaag
      361 gtcgtgaaag ctgtgattga gcagcacatg tcccttaatc aggctgctgc tcactttatg
      421 cttgctggta gtggttctgt agccaggtgg ctgaaggttt atgaagagcg cggagaagct
      481 ggtttacgcg cgctcaagat tggcaccaaa agaaa
//
LOCUS       DAZPEB010000472          514 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZPEB010000472 DAZPEB010000000
VERSION     DAZPEB010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.478"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..279
                     /locus_tag="ACHURJ_004442"
     CDS             <1..279
                     /locus_tag="ACHURJ_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HFR1283082.1"
                     /translation="SLNVYCRNGQVEIDNNIGENALRSVAVGRKNYLFFGSDNGGESA
                     AIIYSLLVTCKLNGVEPEDWLREVIVKLNDWPSNRVHELLPWNFSAVK"
     gene            complement(352..429)
                     /locus_tag="ACHURJ_004443"
                     /pseudo
     CDS             complement(352..429)
                     /locus_tag="ACHURJ_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          125 a          115 c          139 g          135 t
ORIGIN      
        1 agcctgaacg tgtactgtcg taatggccag gtggaaatcg acaacaacat cggtgaaaac
       61 gcgctgcgat cggtggcggt tggaagaaaa aattatctct tcttcggttc tgacaacggt
      121 ggagaaagcg cggcgattat ctacagtctg ctggtcacct gcaaactgaa cggagtggag
      181 ccggaggact ggttgcgcga agtgatcgtg aagctcaatg actggccgtc gaaccgagtg
      241 catgaactgc tgccctggaa cttctcagct gtaaaataat ccttccgcta cgtacttctc
      301 tgggcgctta ccactcaacg gcatcgtcaa aattatcaaa gcgcagaaca tagatttatc
      361 atgtgtttta tggatggctt tctccatcag gcatcgttcg tcacgggaaa ttggtgctat
      421 gatcggcatt gatcagtccg gttggtgatt tgttttgatt tggcgattga tcagatcgca
      481 caatccgggc tgagttccct ctaagtgatc tagg
//
LOCUS       DAZPEB010000473          511 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZPEB010000473 DAZPEB010000000
VERSION     DAZPEB010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.480"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(107..292)
                     /locus_tag="ACHURJ_004444"
     CDS             complement(107..292)
                     /locus_tag="ACHURJ_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005006008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1283083.1"
                     /translation="MIMVAVAAAVTDALLVFFRQLAPFVVLPALGIFIIHGTVRQAVG
                     FIVMPERDQALMSLTVI"
     gene            282..461
                     /locus_tag="ACHURJ_004445"
                     /pseudo
     CDS             282..461
                     /locus_tag="ACHURJ_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238933.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS domain-containing protein"
BASE COUNT          154 a          121 c          140 g           96 t
ORIGIN      
        1 tattaaaaat gtacagaact ctaaaaataa atgtaaatca cagtaagaca gaaccaagca
       61 gaaatcgtgg gcgtggttcg caccctgacg gagggtgtag cgtgtactag atcacagtga
      121 gcgacatcaa agcttggtca cgttctggca ttacgatgaa gccgaccgcc tgacgcaccg
      181 taccgtgaat gatgaagatg ccgagtgctg gcagtacaac gaacggggct agctgacgga
      241 aaaagacgag cagcgcatcc gtgacggcgg cagccacagc caccattatc atgatgaaaa
      301 accagatgga gccggaatac acgccgctgc gtaaaattca cctgtaccac tgcgaccacc
      361 ggggactgcc gctggcgctg atacgcagtg acggccgaac cggatggcgt gtagagtatg
      421 acgaatgggg caacctgctg agtgaggata acccgcacag ggaaagatca tcagaagttc
      481 actttttgta ctaaataatt cgcattttat g
//
LOCUS       DAZPEB010000474          509 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZPEB010000474 DAZPEB010000000
VERSION     DAZPEB010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.481"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            15..119
                     /gene="mgtT"
                     /locus_tag="ACHURJ_004446"
     CDS             15..119
                     /gene="mgtT"
                     /locus_tag="ACHURJ_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFR1283084.1"
                     /translation="MNGDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(234..485)
                     /gene="dgcZ"
                     /locus_tag="ACHURJ_004447"
                     /pseudo
     CDS             complement(234..485)
                     /gene="dgcZ"
                     /locus_tag="ACHURJ_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
BASE COUNT          134 a          120 c          109 g          146 t
ORIGIN      
        1 aaatgggatg actaatgaac ggagataatc cctcacctaa ccggcccctt gttacagttg
       61 tgtacaaggg gcctgatttt tatgacggcg aaaaaaaacc gccagtaaac cggcggtgaa
      121 tgcttgcatg gatagatttg tgttttgctt ttacgctaac aggcattttc ctgcactgat
      181 aacgaatcgt tgacacagta gcatcagttt tctcaatgaa tgttaaacgg agcttaaact
      241 cggttaatca cattttgttc gtcaataaac atacagcgat ttcttcccgt ttgcttaccc
      301 tcatacattg cccggtctgc tcttccaatg accacatcca gaggctcttc aggaaatgcg
      361 cgactgacac ctgctgtcac ggtaatgttg atatgccctt cagaatgtgt gatggcatgg
      421 ttatcgacta actggcaaat tctgacacct gcacgacatg cttcttcatc attagccgct
      481 ttgaggtgta ctggcaatag cggacacta
//
LOCUS       DAZPEB010000475          508 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZPEB010000475 DAZPEB010000000
VERSION     DAZPEB010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.482"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..124
                     /locus_tag="ACHURJ_004448"
     CDS             <1..124
                     /locus_tag="ACHURJ_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFR1283085.1"
                     /translation="TRKMSQGKRHNQALIALARRRCDVLFAMMRDGTFYTPQGS"
     gene            complement(148..309)
                     /locus_tag="ACHURJ_004449"
                     /pseudo
     CDS             complement(148..309)
                     /locus_tag="ACHURJ_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(353..>508)
                     /locus_tag="ACHURJ_004450"
     CDS             complement(353..>508)
                     /locus_tag="ACHURJ_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR1283086.1"
                     /translation="SLSHYRFKSRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFRI
                     KYYQMTA"
BASE COUNT          133 a          128 c          124 g          123 t
ORIGIN      
        1 cacccgcaaa atgagtcagg gaaaacgaca caatcaggcg cttatcgccc tggcgagacg
       61 acgctgcgac gttctgttcg ccatgatgcg cgacgggact ttttataccc cgcaggggtc
      121 ataacatgct tgacaactta ataggggcac cccctcatgc ctgagcatcg catgcaggcg
      181 acgatagcca taacactggt aattacggtt aaagatatct gtcagcatct tacgtatcgt
      241 ggcgtattta tcaccggctt tcagtgcagc ccggtgataa aaataactgc tacgggcaag
      301 gcccagaact gaggtgtact ggcaatagcg gacaccacca tttgttcttt ttctaagcag
      361 tcatctgata atattttatc ctgaaggctg ccggggagat attccccaga cgagagtgac
      421 gacgctgacg attgtagaaa atctcaatgt attcccgtat tactgagatg gcttcatccc
      481 ggctcttaaa acgatagtgg ctcaggct
//
LOCUS       DAZPEB010000476          490 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZPEB010000476 DAZPEB010000000
VERSION     DAZPEB010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.483"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..>490)
                     /locus_tag="ACHURJ_004451"
     CDS             complement(<1..>490)
                     /locus_tag="ACHURJ_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFR1283087.1"
                     /translation="QRAEEARQQAISGGTEPSAFPDTLPGYTEYGRDNGIRLSAVWLT
                     HDPEYPENLPAAPLVRYGWTPRGELAAVYDRSNTQVRSFTYDDKYRGRMVAHRHTGRP
                     EIRYRYDSDGRVTEQLNPAGLSYTYQYEKDRITITDSLNRREVLHTQGEAGLKRVVKN
                     HPE"
BASE COUNT           77 a          158 c          145 g          110 t
ORIGIN      
        1 ctccgggtga tttttcacca cccgcttcag cccggcttca ccctgcgtgt gcagcacttc
       61 acggcggttc aggctgtcgg tgatggtgat gcggtccttc tcatactgat acgtgtagct
      121 taagcctgcc gggttaagct gttctgtcac ccgcccgtcg ctgtcgtaac ggtaacggat
      181 ttccggccgg cccgtgtgac ggtgcgccac catccggccc cggtatttat catcgtaagt
      241 aaagctacgc acctgtgtat tgctgcggtc atacactgcc gccagttcgc cgcgcggcgt
      301 ccagccatag cgcaccagcg gcgcggcagg taaattctcc gggtattccg ggtcgtgcgt
      361 cagccacacg gcagacagac ggatgccgtt gtcccggcca tattcggtgt aacccggcag
      421 ggtatcagga aaagcggacg gttccgtccc gccggaaatg gcctgctgcc gggcttcttc
      481 tgcccgctgc
//
LOCUS       DAZPEB010000477          490 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZPEB010000477 DAZPEB010000000
VERSION     DAZPEB010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.484"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(410..475)
                     /locus_tag="ACHURJ_004452"
                     /pseudo
     CDS             complement(410..475)
                     /locus_tag="ACHURJ_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          152 a           87 c           85 g          166 t
ORIGIN      
        1 ctgataaatg aagaaaaacc gtattcatcg ccattaccac caccttacct atattactca
       61 ttaatgtatg tgcgaaaatt acgccggaaa atcattaaaa aaagcggatt tttgcatgtt
      121 gttttgtttt tgcgaggcgt tttccagaaa taataagagt gttgcagttg tttcatttat
      181 tactcaaaaa tggtttagtt atcgcccagg atgaattatg ggtgctatga atatattata
      241 ttcagagata tttatttgca taatctgaat ggtgtagccg aaaaacgtca gcatcctaac
      301 agcaccagga tttaaggtga aattaatctt tcatcacaga ccggtcagta ctgccaccaa
      361 ccggtcttta cttgctcatc gttataattt tgttggtaac agttcgggaa agttgttttt
      421 ctaaattaac aacagaataa ctttcttgtt taatgagatt aatattggca accattatag
      481 cctccttccc
//
LOCUS       DAZPEB010000478          470 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZPEB010000478 DAZPEB010000000
VERSION     DAZPEB010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.485"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
BASE COUNT          139 a           94 c           88 g          149 t
ORIGIN      
        1 ttgtgggcca taaatattcc aaaaaaatag cccgctccgt ttattaccgg aacgggcgag
       61 agcatgacat tgtctgtaat tataatgaga ctgctgattc tacttatttt taatttcaag
      121 aaaagcgttt tctacaaaga attatgagat ttaatgtttt gtttagacaa tattgtcatt
      181 ctgttcaata atgaaagtct tcatcaacaa caagtaaaaa caacgtccct ctcatcgaac
      241 cggacttgca gatttcccgc atccggctct cttgcaacac atcagacctt cacgttcggg
      301 cggttatggg gaaggtccag caagctgtat gagccctaac tgtttgaata ccagtagatt
      361 accagaaact tctatcgatt gaagacatta ccaactagcg ttactaaatg ttgaccaagt
      421 tttgttcact tgtctggttt tgatagtagc attctgcaga gtattttgat
//
LOCUS       DAZPEB010000479          468 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZPEB010000479 DAZPEB010000000
VERSION     DAZPEB010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.486"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..>468
                     /locus_tag="ACHURJ_004453"
     CDS             <1..>468
                     /locus_tag="ACHURJ_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR1283088.1"
                     /translation="ARLASPPKAAAEALKELGVSVSDAGGKMRPMEDVLADLYKATRK
                     YGEVDRVSFFKDIAGEEAFTSFMALVDAAGDGSLPKLRKELEGARGEAERTAKVMANN
                     LDGDLKSLSSAWEGLRIRIADLIDGPLRSVTQWLTRVVSKVTALAQAHPALTRQ"
BASE COUNT          104 a          122 c          149 g           93 t
ORIGIN      
        1 gctcgtctgg catcaccgcc aaaggcggca gcagaggcgc tgaaagagct tggcgtgtct
       61 gtctcggatg ccgggggcaa aatgcgcccg atggaggatg tgctggctga cctttataaa
      121 gctacccgca aatacgggga agttgaccgg gtatcgttct ttaaggacat tgccggagaa
      181 gaggctttca catcatttat ggccctcgtt gatgcggcag gtgacggctc cttacccaaa
      241 ctgagaaaag aacttgaagg cgcgcgcggt gaggctgaac gcacggcaaa ggttatggcc
      301 aacaaccttg acggcgatct gaaatcactc agcagtgcat gggaagggtt gcgcatccgc
      361 attgcagatc tgattgacgg tccgctgcgt tctgtcacgc agtggctcac gcgtgtggta
      421 tcaaaggtga cggcgctggc gcaggcccat cccgcactga cgcgccag
//
LOCUS       DAZPEB010000480          463 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZPEB010000480 DAZPEB010000000
VERSION     DAZPEB010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.487"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..>463)
                     /locus_tag="ACHURJ_004454"
     CDS             complement(<1..>463)
                     /locus_tag="ACHURJ_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1283089.1"
                     /translation="LLGIVNIIWYSHYKVSGSVYTNAYRGPMETGKIALCSAFIFLVL
                     FAKNEMRTKIKFGKLILFASLATQLLFFAHAMWQHFYLNVDRVALSASHATTAGYIIL
                     FPSLLASILILKSDFRHKTTLYTINFMLSLCAVIVTETRAAILVFPFFALIL"
BASE COUNT          165 a           74 c           94 g          130 t
ORIGIN      
        1 ttaatattaa cgcaaagaat ggaaacacta atatggctgc acgcgtctca gttactatga
       61 cagcacataa gctaagcatg aagttaattg tatataatgt tgttttatgt ctaaagtcgg
      121 attttaaaat gagaattgat gccagtaaag aaggaaaaag gatgatgtag cctgctgttg
      181 tagcgtggga agctgataat gcaacacggt cgacgtttaa atagaaatgt tgccacatgg
      241 catgcgcaaa aaaaagtaac tgcgttgcca gggatgcgaa cagaattagt ttcccaaatt
      301 ttatttttgt tctcatctca tttttagcaa aaagaactaa gaaaataaaa gcgctacaca
      361 aggcaatttt tccagtttcc attgggccac gatatgcatt ggtataaaca gagcctgaaa
      421 ctttataatg tgaataccag atgatattta cgatacccaa aag
//
LOCUS       DAZPEB010000481          463 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZPEB010000481 DAZPEB010000000
VERSION     DAZPEB010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.488"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(63..131)
                     /locus_tag="ACHURJ_004455"
                     /pseudo
     CDS             complement(63..131)
                     /locus_tag="ACHURJ_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461174.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DinI family protein"
     gene            102..314
                     /locus_tag="ACHURJ_004456"
                     /pseudo
     CDS             102..314
                     /locus_tag="ACHURJ_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
BASE COUNT          143 a           90 c          121 g          109 t
ORIGIN      
        1 tttgtgaatt aaatcaatta tggcgatgaa ttacaagggg ttaaatgctg ccgcagcata
       61 gcgatattga aatagcctgg tatgcttcga tacagcagga gctgaatgtc tggaagaccg
      121 tcaccacaca gttctacatc caggaattca gtgagcatat tgcgccactt caggatgctg
      181 tagatctgga gatcgcaacg gaggaagaaa actcgttgct ggaagcctag aagaagtatc
      241 gggtgttgct gaaccgtgtt gatacatcaa ctgcacctga tattgagtgg ccggaagaac
      301 cagacacaat gtaagcgaaa aagaaaaacc gcagacgcga cgtatgcagg acgtgctgcg
      361 gttggctggt gaactttcga tagtgcgagt attgaatgat ttccagccgt taccgatttt
      421 atataactgc agtggaatta ttcaacacac aggcaaaacg gaa
//
LOCUS       DAZPEB010000482          455 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZPEB010000482 DAZPEB010000000
VERSION     DAZPEB010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.489"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..207
                     /locus_tag="ACHURJ_004457"
     CDS             <1..207
                     /locus_tag="ACHURJ_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR1283090.1"
                     /translation="PMMKNVAKTIGKRLYGILNAMRHGVSNGNAEALNSKIRLLRIKA
                     KGYRNRERFKLGVMFHYGKLNMAF"
     gene            237..455
                     /locus_tag="ACHURJ_004458"
                     /pseudo
     CDS             237..455
                     /locus_tag="ACHURJ_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912930.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          128 a          102 c          132 g           93 t
ORIGIN      
        1 cccatgatga agaatgtggc taaaacgata gggaaaaggt tgtacggtat cctgaacgcc
       61 atgcgccatg gcgtatcaaa cggaaatgcg gaagcactga acagcaagat caggctgctg
      121 agaataaaag ccaagggcta ccgcaaccgg gagcgcttta agctgggagt gatgttccac
      181 tatgggaaac tgaatatggc gttctaagcc ttcccaccat gatcggggaa gacccagata
      241 tgaacaggca cattcagagt tactttaaag agcttactga acgtctgagg tgttagagcc
      301 atgactgccg cagcattgct ggcggaaatg cctgaatcga gcagcctctc gagacgtgaa
      361 atcagcgcac ttgtcggtgt tgctcaggta aaccgggact cgggaactct gcgcggtcga
      421 cgaaccattt tcggtgattg tgctggggaa gaaca
//
LOCUS       DAZPEB010000483          448 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZPEB010000483 DAZPEB010000000
VERSION     DAZPEB010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.490"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..332)
                     /locus_tag="ACHURJ_004459"
     CDS             complement(<1..332)
                     /locus_tag="ACHURJ_004459"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFR1283091.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPE"
BASE COUNT          140 a           95 c          108 g          105 t
ORIGIN      
        1 ctttccggta aaaataaaaa cggtaaatag agcgcaatat cggaaataac atcgccagtt
       61 tcattcaaaa tcgcccccag acgtgtttgc tgattgcact cacgcgccaa catgccatcc
      121 agcgcattga gcgccatacg gataaaaagc acgatgggca atagcaaaaa gaggatgggt
      181 tgtgccacca acaccaacag caatccggta aaaagagaaa gcgccagtgc cgtaagagtg
      241 atgtgattcg ctgtaacgtg gtgcttataa agccaaaaca tcgtcggcct taacagcgac
      301 tgaaagagcg gttttatctg gtagagcgtc atttatcatt ccgtgatatt cagtttgctc
      361 tatggtgcat caattgtgat ggcggagata tcacaacgcg gagcatcaac gtaaattcgg
      421 aatatatcaa gatgtggtgg atggtttt
//
LOCUS       DAZPEB010000484          448 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZPEB010000484 DAZPEB010000000
VERSION     DAZPEB010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.491"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..>448
                     /locus_tag="ACHURJ_004460"
     CDS             <1..>448
                     /locus_tag="ACHURJ_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR1283092.1"
                     /translation="YYNWVQHEPSDRKQSDERLKLEIKVAHIRTRETYGTRRLQTELA
                     ENGIIVGRDRLARLRKELRLRCKQKRKFRATTNPNHNLPVAPNLLNQTFAPTAPNQVW
                     VADLTYVATQEGWLYLAGIKDVYTCEIVGYAMGERMTKELTGKALFM"
BASE COUNT          128 a          110 c          124 g           86 t
ORIGIN      
        1 tattacaact gggtacagca tgaaccctca gacagaaaac aaagtgatga gcggctaaaa
       61 ctggagatta aggtggcaca tatccgcact cgcgaaacat atggaacccg gcggctccag
      121 acggagctgg cagagaatgg catcatcgtt ggtcgtgacc gactggcacg tcttcgtaag
      181 gagctaaggc tacgctgtaa gcagaaacgc aagttcagag cgactacgaa cccgaaccac
      241 aatctgccag ttgcgccaaa tctgctgaac cagacgttcg ctcctacagc accaaatcag
      301 gtctgggtgg cggacctgac gtatgttgcc acacaggagg gatggttgta cctcgctggc
      361 atcaaagatg tttatacgtg cgaaattgtc ggctacgcca tgggagagcg catgacaaaa
      421 gagctgacag gtaaagccct gtttatgg
//
LOCUS       DAZPEB010000485          441 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZPEB010000485 DAZPEB010000000
VERSION     DAZPEB010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.492"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            92..>441
                     /locus_tag="ACHURJ_004461"
     CDS             92..>441
                     /locus_tag="ACHURJ_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1283093.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAA"
BASE COUNT          132 a           83 c          101 g          125 t
ORIGIN      
        1 ttgatttaaa tgacggtcaa ttattgttat tattaagtct aatgtcattt aaaggcatta
       61 tcatatttac gaagagatca ggatgatgac gatgaaaaaa agtgtattga cggcgtttat
      121 aactgtggta tgtgcaacgt ccagcgttat ggcagctgat gataatgcta tcacggatgg
      181 caaagtaaca tttaatggta aagttattgc tccagcttgt accctggtag ctgcgacgaa
      241 agattcagta gtcacattgc caaatgtcag cgccaccaag ttgcaaacta atggagcagt
      301 ctctggcgtt aaaactgatg taccaattgc cttagaaggc tgtgatgtta ctgtaactaa
      361 aaatgcgacg tttaccttta gtggtacggc tgacggcgtg caacctacag catttgccaa
      421 ccaggctacc accgatgcag c
//
LOCUS       DAZPEB010000486          438 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZPEB010000486 DAZPEB010000000
VERSION     DAZPEB010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.493"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..>438
                     /locus_tag="ACHURJ_004462"
     CDS             <1..>438
                     /locus_tag="ACHURJ_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFR1283094.1"
                     /translation="RHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFH
                     VHTHTHTRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQ
                     VAEELGFYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRR"
BASE COUNT          151 a           70 c          106 g          111 t
ORIGIN      
        1 tcgtcactct cctggaaagg aatattctca tcgcgattgc gaacatcaaa tagcgacagg
       61 gaatgctgat aatgttatgc ttcgctggtc tgaagtaaac gaaatgttgc aatctggtct
      121 ggtcgaattt catgttcata cgcatactca tacacgctgg gataaaaagt tcagttcacg
      181 agaagaacag tgtaaacatc ttcggcagga cttgttgagt gggagagaat atttgaaaga
      241 aatgacagga aaatgcagta aacatttgtg ttggccggaa ggttattata ataaggatta
      301 tattcaggtc gctgaagaac ttggattcta ttatttatat acaacagaaa gaagaatgaa
      361 tgctcctgct aaaggaacta cacggatagg aagaataagc accaaagaac gggaaagttg
      421 cgcatggctg aaaagacg
//
LOCUS       DAZPEB010000487          432 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZPEB010000487 DAZPEB010000000
VERSION     DAZPEB010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.494"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            84..290
                     /locus_tag="ACHURJ_004463"
                     /pseudo
     CDS             84..290
                     /locus_tag="ACHURJ_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611787.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
BASE COUNT          129 a           92 c           91 g          120 t
ORIGIN      
        1 cagttttacg atgtccgata atttataaca tttcgtacgg ttggaaaaat gttactaaat
       61 gcccgtcagg cagggaggcc gatatgcccg ttgactttct gaccactgag cagactgaaa
      121 gctatggcag attcaccggt gaaccggatg agcttcagct ggcacgatat tttcaccttg
      181 atgaagcaga caaggaattt atcggaaaaa gcagaggtga tcacaaccgt ctgggcattg
      241 ccctgcaaat tggatgtgtc cgttttctgg gcaccttcct caccgatatg aatcatacct
      301 agattctacg tcagtacttc aaaaagcata atcaaagcct tgataaatat gcattccttc
      361 gaaattcagc tttcacccat tgggtgaaag aaaagtgctc aaaaatatgt taaattatca
      421 gcttttatga ct
//
LOCUS       DAZPEB010000488          432 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZPEB010000488 DAZPEB010000000
VERSION     DAZPEB010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.495"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(150..335)
                     /locus_tag="ACHURJ_004464"
                     /pseudo
     CDS             complement(150..335)
                     /locus_tag="ACHURJ_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
BASE COUNT          116 a          120 c           80 g          116 t
ORIGIN      
        1 cggtgcattt atctccagca ccagactttc tatctcaacg ccatacgttg catttttggt
       61 aatatccgtc agcgtcagtg catttagtcc cactgccaga ctgtctttta tggcctggaa
      121 tgccgggcca gtacgatgac gtagtatcac tccggctcag ttgcaccact gaccaccaca
      181 tcaccttctg ctgcaatcgc ctgcatcagg gtataagggg ttatggccac cggactacca
      241 aacggctgcc agccctcttt cagtttatgt gtcagctttt ccgcaagatc tgacggcggc
      301 gccgccctga caacatcata gtgtttaaat gccatggttc tttccaccat ctgaaaaata
      361 attctttaaa atacctgaca tgtaatacag aaaaaacaca aaaccatacc ttaaataaaa
      421 acctgattat ca
//
LOCUS       DAZPEB010000489          425 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZPEB010000489 DAZPEB010000000
VERSION     DAZPEB010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.496"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..380)
                     /locus_tag="ACHURJ_004465"
     CDS             complement(<1..380)
                     /locus_tag="ACHURJ_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFR1283095.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQAR
                     QRLGSEAVRRVFTKTAQLWHNATP"
BASE COUNT          108 a          107 c          128 g           82 t
ORIGIN      
        1 tgcggcgtgg cgttatgcca gagctgcgct gttttcgtga acacgcggcg gacagcctca
       61 cttcccaggc gctggcgggc ctgaataacg gcactggggg caacgaaggg gcgattgccc
      121 ggcagcatga tgtccaggcg attcacaatc tggtgaagag gttctttacg ctcaagcgcc
      181 atgccaacaa tacaccagac catcatttcg aggggaagac ggcgcttgcg tagcgttaca
      241 gtacctgatt cggcaaggca acgagagatg agttcggggt cgaggtaatc ccccagagaa
      301 gtcagtgggt tacgcagaga atcgtaacgg gataccagat caagagcctg tccaatgtgc
      361 ataaaaaaat ccggaaacaa gtgagcgttt ccggattctt acacagccac tggatcggtc
      421 aactg
//
LOCUS       DAZPEB010000490          421 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZPEB010000490 DAZPEB010000000
VERSION     DAZPEB010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.497"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..227
                     /locus_tag="ACHURJ_004466"
     CDS             <1..227
                     /locus_tag="ACHURJ_004466"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1283096.1"
                     /translation="LYHRQSSDIEIDDTEMTLTILLVSVISNLTSPVPRRGEGYQAQG
                     LIIDTTVKIAFSKGNVIKFRGFQSENHTTY"
     gene            325..>421
                     /locus_tag="ACHURJ_004467"
     CDS             325..>421
                     /locus_tag="ACHURJ_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1283097.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKTNPNKATKAL"
BASE COUNT          162 a           70 c           79 g          110 t
ORIGIN      
        1 gactctatca tcgccaatca tcagacattg aaatagacga cactgagatg acgcttacaa
       61 ttcttttggt ttctgtaata agtaatctga catcccccgt gcccagaaga ggagagggat
      121 atcaagccca agggttaata attgatacta cggttaaaat tgccttcagt aaaggaaacg
      181 tgattaaatt caggggattt cagagtgaaa accatacaac atattaaaat ctgttcattt
      241 agctataata catacaagaa agtgtccagt tagataatgt tatctaaata accacagata
      301 aaaacgcaca taattggggg ggggatgaaa atacctgaag cagtaaatca tattaatgtt
      361 cagaataata ttgatcttgt tgatggaaaa acgaatccga ataaagcaac aaaagcatta
      421 c
//
LOCUS       DAZPEB010000491          410 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZPEB010000491 DAZPEB010000000
VERSION     DAZPEB010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.498"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            74..349
                     /locus_tag="ACHURJ_004468"
                     /pseudo
     CDS             74..349
                     /locus_tag="ACHURJ_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
BASE COUNT          131 a           69 c           89 g          121 t
ORIGIN      
        1 gtcatttata aatgacgccg tttaaaattc gtttagaaaa tacgtttgac gataattaaa
       61 aggattctgt taaatgaaat taaaaatagt tgcggtggtt gtaactggtt tgttagctgc
      121 gaacgtagca cacgctgccg aagtctataa caaggatggt aataaactcg acctttatgg
      181 caaggttacc gctctacgtt attttactga tgataagcgt gacgatggtg ataaaactta
      241 tgcccgtctc ggctttaaag gagaaacgca aatcaatgat caaatgattg gttttggtca
      301 ctgggaatat gattttaaag gctataacga tgaagccaac ggctcgcgcg gcaagaatct
      361 ctgattgagt taagtgccca tcacaatatg atcactgacc ttatattaca
//
LOCUS       DAZPEB010000492          407 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZPEB010000492 DAZPEB010000000
VERSION     DAZPEB010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.499"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            196..>407
                     /locus_tag="ACHURJ_004469"
     CDS             196..>407
                     /locus_tag="ACHURJ_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR1283098.1"
                     /translation="MARYDLPDEAWTIIKPLLPPEPTTPRAGRPWAEHRKIINGMFWV
                     LCSGAPWRDLPERYGAWKTVYNRFNR"
BASE COUNT          130 a           93 c           90 g           94 t
ORIGIN      
        1 aatatgatag ataaaataga gacgcagaac agggcatgct cataacatga tgataacata
       61 aaaaattagt aacaataaat gaaagaatca tgggcgctca acaaaaagag agctaaatag
      121 ctgcgcggaa tagtaggctg tgtcccttaa tcatctgagt tatagtaact gtctgtttac
      181 acaacatatt gaactatggc tcgctacgac cttcccgatg aggcatggac catcatcaag
      241 cccctgttac ctcctgaacc caccacacca cgggccggac gcccatgggc tgagcaccgt
      301 aaaatcatca atggcatgtt ctgggtgtta tgttccggtg caccatggcg cgatttaccc
      361 gaacgatatg gggcatggaa aactgtttat aaccgcttta accgatg
//
LOCUS       DAZPEB010000493          403 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZPEB010000493 DAZPEB010000000
VERSION     DAZPEB010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.500"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            97..>403
                     /gene="hlyE"
                     /locus_tag="ACHURJ_004470"
     CDS             97..>403
                     /gene="hlyE"
                     /locus_tag="ACHURJ_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HFR1283099.1"
                     /translation="MTEIVADKMVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETI
                     KELSRFKQEYSQAASVLVGDIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLFD
                     "
BASE COUNT          137 a           64 c           84 g          118 t
ORIGIN      
        1 cacccggcta actatgaatt agatgaagta aaatttatta atagttgtaa aacaggagtt
       61 tcattacaat ttatatattt aaagaggcga atgattatga ctgaaatcgt tgcagataaa
      121 atggtagaag tagttaaaaa cgcaatcgaa accgcagatg gagcattaga tctttataat
      181 aaatatctcg atcaggtcat cccctggcag acctttgatg aaaccataaa agagttaagt
      241 cgctttaaac aggagtattc acaggcagcc tccgttttag tcggcgatat taaaacctta
      301 cttatggata gccaggataa gtattttgaa gcaacccaaa cagtgtatga atggtgtggt
      361 gttgcgacgc aattgctcgc agcgtatatt ttgctatttg atg
//
LOCUS       DAZPEB010000494          402 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZPEB010000494 DAZPEB010000000
VERSION     DAZPEB010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.501"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            9..402
                     /gene="ospC4"
                     /locus_tag="ACHURJ_004471"
                     /pseudo
     CDS             9..402
                     /gene="ospC4"
                     /locus_tag="ACHURJ_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
BASE COUNT          141 a           80 c           62 g          119 t
ORIGIN      
        1 ctggaaatat aggggcaaat ccacttgttg atggaaaaac gaatccgaat aaagcaacaa
       61 aagcattaca aaaaatatat tacgcgtaac aaactcatct tcttctggca taagtgaaaa
      121 acatttagat cactgtgcta atactgtgaa aaacttttta aggaagtcta tagcagcgca
      181 aagttatagc aaaatgtttt ctcaagggac cagtttcaaa tcactaaatc tttctattga
      241 agcgccatct ggagcacggt catcatttcg gtcacttgag catctcgata aggtgtccag
      301 acactatatc tctgagataa tacaaaaagc tcatcctctt tcttcagacg aacgtcactt
      361 actttctatt attattaatt ctaattttaa cttcagacat ca
//
LOCUS       DAZPEB010000495          372 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZPEB010000495 DAZPEB010000000
VERSION     DAZPEB010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.502"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..360)
                     /locus_tag="ACHURJ_004472"
     CDS             complement(<1..360)
                     /locus_tag="ACHURJ_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR1283100.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKQARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQK"
BASE COUNT           89 a           84 c           90 g          109 t
ORIGIN      
        1 tttctgctta attttgcttt cacatttttg taagtcttct gccggattgt gcgacgtctg
       61 caatatcacg tcgaaacctt cctcttcagc tttggcggtg atggcatgca aaacttcaga
      121 gaaaaacgga ttacccgccg tagttttggt cgaacgggta gaaatcacca taatggcatc
      181 aaaacccgaa gaggtcagcg cgcgggcctg cttgtttggc tgatactgta attcttcaat
      241 ggcccgtaac actttttcgc gcgcttccgg ggagatatta gtttgcttat tcagcacgcg
      301 tgatacggtg gattttgata cgcctgcaac cctggcaata tcataaatag taggggacat
      361 aggtcaggaa ct
//
LOCUS       DAZPEB010000496          370 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZPEB010000496 DAZPEB010000000
VERSION     DAZPEB010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.503"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            1..370
                     /locus_tag="ACHURJ_004473"
                     /pseudo
     CDS             1..370
                     /locus_tag="ACHURJ_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          121 a           92 c           90 g           67 t
ORIGIN      
        1 tcagggcaaa acaccaaaag cctctccgat aacaccagaa caaatcgaaa tacgtaagct
       61 gaggaaaaag ctacaacgca ttgaaatgga gaatgaaata ttaaaaaagg ctaccgcgct
      121 cttgatgtca gactccctga acagttctcg ataatcggga aactcagagc gcattatcct
      181 gtggtcacac tctgccaagt gttcggggtt catcgcagca gctacagata ctggaaaaac
      241 cgtcctgaaa aaccagacgg cagacgggct gtattacgca gtcaggtact tgagctacat
      301 ggcatcagcc atggttcggc cggagcaaga agcatcgcca caatggcaac ccggagaggc
      361 taccagatgg
//
LOCUS       DAZPEB010000497          370 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZPEB010000497 DAZPEB010000000
VERSION     DAZPEB010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.504"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..242)
                     /locus_tag="ACHURJ_004474"
     CDS             complement(<1..242)
                     /locus_tag="ACHURJ_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFR1283101.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
BASE COUNT          114 a           68 c           76 g          112 t
ORIGIN      
        1 ttaatggata aatccatcaa tttgtggaca ttttcgtcgt cccaggtctg gcagaaagtg
       61 gccaggttct gacgagcgtt gccatcaaga tataattcgt cattgataat ctggaatgcg
      121 acatcgtcgc gcatttcgtg cagcggaaaa cgttttgatt ctgcgatagt ggaaatagac
      181 ttcgcaccaa aacgtgaatc gagtagttcc gaccttaaat ccgttacttg cttcttatcc
      241 attttaaact ccttaaaatg attggatcgc attaaaaaag taggatttat cgataaagta
      301 agcaagtgca aaggactcgt gtttaaataa caaaatccta atgttattta tcgtgagata
      361 ttacgcgaat
//
LOCUS       DAZPEB010000498          366 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZPEB010000498 DAZPEB010000000
VERSION     DAZPEB010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.505"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..>366
                     /gene="leuE"
                     /locus_tag="ACHURJ_004475"
     CDS             <1..>366
                     /gene="leuE"
                     /locus_tag="ACHURJ_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFR1283102.1"
                     /translation="VGAIFIVLVPGPNTLFVLKNSVSSGMKGGYLAACGVFIGDAVLM
                     FLAWAGVATLIKTTPILFNIVRYLGAFYLLYLESKILYATLKGKNNEAKSDEPQYGAI
                     FKRALILSLTNPKAILFYVS"
BASE COUNT           87 a           72 c           92 g          115 t
ORIGIN      
        1 ggttggggcc atttttattg tgttggtgcc tgggccaaat accctgtttg tactcaaaaa
       61 tagcgtcagt agcggtatga aaggcggtta tcttgcggcc tgcggtgtat ttattggcga
      121 tgcagtattg atgtttctgg catgggctgg agtggcgaca ttaattaaga ccaccccgat
      181 attattcaac attgtacgtt atcttggtgc gttttatttg ctctatctgg agagtaaaat
      241 tctctacgcg accctgaagg gtaaaaataa cgaggccaaa tccgatgagc cccaatacgg
      301 ggccattttt aaacgcgcgt taattttgag cctgactaat ccgaaagcca ttttgttcta
      361 tgtgtc
//
LOCUS       DAZPEB010000499          348 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.506, whole genome shotgun sequence.
ACCESSION   DAZPEB010000499 DAZPEB010000000
VERSION     DAZPEB010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.506"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..>348
                     /locus_tag="ACHURJ_004476"
     CDS             <1..>348
                     /locus_tag="ACHURJ_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR1283103.1"
                     /translation="LNWHPHVHVSVTCGGLNKHGQWKKLSFLKDAMRSRWMWNMRQLL
                     LKAWSEGLAMPESLSHITTESQWRSLVLKAGGKYWHVYMSKKTAGGRNTARYLGRYLK
                     KPPIAASRLAHYNG"
BASE COUNT           87 a           78 c          113 g           70 t
ORIGIN      
        1 tctcaactgg catccgcatg tacatgtgtc tgtaacctgt ggaggtctga ataagcatgg
       61 tcagtggaaa aagctgagct tcctgaaaga cgcgatgcgt tcacggtgga tgtggaatat
      121 gcggcagctg cttctgaaag cgtggtcaga ggggctggcg atgccggagt cgttgtcaca
      181 tatcacgacg gaatcacagt ggagaagcct ggtgctgaaa gccggaggaa aatactggca
      241 tgtgtacatg tcgaaaaaaa cggccggcgg gaggaatacg gcgcgctacc tgggtcgtta
      301 tctgaagaag ccgccgatag cggcctcccg gctggcacat tacaacgg
//
LOCUS       DAZPEB010000500          348 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZPEB010000500 DAZPEB010000000
VERSION     DAZPEB010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.507"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..>348
                     /locus_tag="ACHURJ_004477"
     CDS             <1..>348
                     /locus_tag="ACHURJ_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR1283104.1"
                     /translation="RFETEPGRQMQVDWGTMRNGRSPLHVFVAVPGYSRMLYIEFTDN
                     MRYDTLETCHRNAFRFFGGVPREVLYDNMKTVVLQRDAYQTGQHRFHPSLWQFGKEMG
                     FSPRLCRPFRAQT"
BASE COUNT           71 a           92 c           95 g           90 t
ORIGIN      
        1 ttcggttcga aactgaaccc ggacgacaga tgcaggttga ctggggcact atgcgtaatg
       61 gtcgctcacc gcttcacgtg ttcgttgctg ttcccggata cagccgaatg ttgtacatcg
      121 aattcactga caatatgcgt tatgacacgc tggagacctg ccatcgtaat gcgttccgct
      181 tctttggtgg tgtgccgcgc gaagtgttgt atgacaatat gaaaactgtg gttctgcaac
      241 gtgacgcata tcagaccggt cagcaccggt tccatccttc gctgtggcag ttcggcaagg
      301 agatgggctt ctctccccga ctgtgtcgcc ccttcagggc acagacta
//
LOCUS       DAZPEB010000501          347 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.508, whole genome shotgun sequence.
ACCESSION   DAZPEB010000501 DAZPEB010000000
VERSION     DAZPEB010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.508"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..>347
                     /locus_tag="ACHURJ_004478"
     CDS             <1..>347
                     /locus_tag="ACHURJ_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001751361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HFR1283105.1"
                     /translation="INDVDSSVIGIVATADDADAKLFPLNKPTLLTRVNDVLGKCGTT
                     GTLYRALKAIADQVSTKVIVVRVAEHKEEDGKTQDQLVIGGSEDDGSYTGMYALLVAE
                     QDESIGYRPRILAA"
BASE COUNT           85 a           81 c          111 g           70 t
ORIGIN      
        1 attaacgatg ttgattccag cgttatcggt atcgtggcaa cggcggatga tgcggacgcg
       61 aagctgttcc cgctgaacaa gcccacactg ctgacccgcg tcaatgacgt gctgggaaaa
      121 tgcgggacaa cggggacgct ttatcgtgcg cttaaggcca tcgcagacca ggtgagcaca
      181 aaggtgatcg tcgttcgcgt ggctgaacac aaagaagaag acggaaagac gcaggatcaa
      241 ctggttatcg gtggttctga ggatgacggc agctatacgg ggatgtatgc gctgcttgtt
      301 gcagagcagg atgaaagcat cggataccgt ccgcgtattc tggccgc
//
LOCUS       DAZPEB010000502          343 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.509, whole genome shotgun sequence.
ACCESSION   DAZPEB010000502 DAZPEB010000000
VERSION     DAZPEB010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.509"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..>343
                     /locus_tag="ACHURJ_004479"
     CDS             <1..>343
                     /locus_tag="ACHURJ_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1283106.1"
                     /translation="FESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRAIR
                     YNFQVDDAKFRRDNVKEIISTLFANKVDVDHPENKYKDFKDLEDKVEKRLQNRQTEYQ
                     NEINQLSAPGVN"
BASE COUNT          133 a           54 c           68 g           88 t
ORIGIN      
        1 gtttgaaagc agtaaagcta tcatggcaga atcatttgca acaggttccg atcatcaggt
       61 tgtaaacgag ctcaacgggg aaagactgag agaacctaac gaagttttta aacgtttagg
      121 tcgagcaata agatataatt ttcaagtgga cgatgcaaaa tttagacgcg ataatgtaaa
      181 agaaataatt tctactttat tcgctaacaa agttgacgtt gaccatcctg aaaataaata
      241 caaagacttt aaggatctcg aagataaagt tgaaaaaagg cttcaaaatc gccagacgga
      301 atatcaaaat gaaattaatc aactatctgc gccaggtgtt aat
//
LOCUS       DAZPEB010000503          337 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.510, whole genome shotgun sequence.
ACCESSION   DAZPEB010000503 DAZPEB010000000
VERSION     DAZPEB010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.510"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
BASE COUNT           97 a           76 c           72 g           92 t
ORIGIN      
        1 tcccgtaaag acaggagtca agttcccgct ccgacataac tgcgtgcaat ggattttgaa
       61 ggaaccgtca gcccattccc cggaggcaaa catgggctgg caaaagtgta gcggtattag
      121 gcacagctat ttaggaaaga gggaccatct ccatctcatt aaatggacca cgccagacaa
      181 ttcactgtca tgctgatgct gcactgtggg gacagaatca ctcatgatga cttcgataat
      241 cgacgacatt attatttggc cgagctttta ggtcatatct caaatcgttt gcctaatatt
      301 gctccatttt ttaataaaaa atcaatcatt tttggat
//
LOCUS       DAZPEB010000504          335 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.511, whole genome shotgun sequence.
ACCESSION   DAZPEB010000504 DAZPEB010000000
VERSION     DAZPEB010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.511"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            88..>335
                     /locus_tag="ACHURJ_004480"
     CDS             88..>335
                     /locus_tag="ACHURJ_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HFR1283107.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWL"
BASE COUNT          110 a           84 c           70 g           71 t
ORIGIN      
        1 ggctgcaatc aatgattatg gcggcagttc cgggacagaa actcgtccac tgcaataaca
       61 aatataaaaa acacaggtca tcagggaatg ccacaacgac accaccaggg acataaacgc
      121 acaccgaaac agttggcgct catcatcaaa cgctgtttgc cgatggtgct cactggcagc
      181 ggcatgcttt gcactaccgc taacgccgaa gagtattatt tcgaccccat tatgctggaa
      241 actacaaaaa gtggtatgca aacaactgat ctgtcacgtt tttcaaaaaa atacgcacaa
      301 ctaccaggaa cttatcaggt tgatatctgg ctgaa
//
LOCUS       DAZPEB010000505          335 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZPEB010000505 DAZPEB010000000
VERSION     DAZPEB010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.512"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..121
                     /locus_tag="ACHURJ_004481"
     CDS             <1..121
                     /locus_tag="ACHURJ_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="reverse transcriptase"
                     /protein_id="HFR1283108.1"
                     /translation="WMQSKYRLSNRKAQRKLALVRKQYPKLFAHWYLLRASNE"
     gene            195..314
                     /locus_tag="ACHURJ_004482"
                     /pseudo
     CDS             195..314
                     /locus_tag="ACHURJ_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
BASE COUNT           85 a           78 c          102 g           70 t
ORIGIN      
        1 gtggatgcag tctaaataca gactttcgaa ccggaaggct cagcgaaagc tggcgctggt
       61 aaggaaacag tatccgaagc tatttgccca ctggtattta ctacgtgcat cgaatgagtg
      121 atcaagagcc gtatgacgcg agagtgtcac gtacggttcc gtgagcagcc ggagagtgaa
      181 actctctccg acggggttcc gatagcggtg gcgaacatgc ggcggtgttg tactcgctga
      241 tcggcacatg ccgtctgaac aatgtggagc cagaaaaatg gctgcgttac gtcattgagc
      301 atatccagga ctgaccggca aaccgggtac gcgat
//
LOCUS       DAZPEB010000506          334 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.513, whole genome shotgun sequence.
ACCESSION   DAZPEB010000506 DAZPEB010000000
VERSION     DAZPEB010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.513"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            1..334
                     /locus_tag="ACHURJ_004483"
                     /pseudo
     CDS             1..334
                     /locus_tag="ACHURJ_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT           74 a           84 c          100 g           76 t
ORIGIN      
        1 ctcctcaaag aagccgttga atatggacgg gcaaaaaagt ggatagcgca cgcgccctta
       61 ttgcccgggg atggggagta agcttagtca gccgttgtct ccgggtgtcg cgtgcgcagt
      121 tgcacgtcat tctcagacga accgatgact ggatggatgg ccgccgcagt cgtcacactg
      181 atgatacgga tgtgcttctc cgtatacacc atgttatcgg agagctgccc acgtatggtt
      241 atcgtcgggt atgggcgctg cttcgcagac aggcagaact tgatggtatg cctgcgatca
      301 atgccaaacg tgtttaccgg atcatgcgcc agaa
//
LOCUS       DAZPEB010000507          333 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.514, whole genome shotgun sequence.
ACCESSION   DAZPEB010000507 DAZPEB010000000
VERSION     DAZPEB010000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.514"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..>333
                     /locus_tag="ACHURJ_004484"
     CDS             <1..>333
                     /locus_tag="ACHURJ_004484"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1283109.1"
                     /translation="IGRDKRIAVNEAIAANMELLSTQHISLLDRIKGNSVTLFCELIL
                     NFRNEIERRQLSTNTMKRHNQRLKIISEYFGGVPVKNIGIREVYSFLEIRAAGSKFAI
                     ANQYRALLS"
BASE COUNT          125 a           49 c           74 g           85 t
ORIGIN      
        1 atcggaagag acaaaagaat tgcagttaat gaggctattg cggcaaatat ggaattatta
       61 tccacgcaac atataagctt actggataga ataaaaggta atagcgtaac attattctgc
      121 gagctgatat taaatttcag aaatgaaata gagcgtagac aattaagcac aaacaccatg
      181 aaaagacaca accagaggtt gaaaatcata agtgaatact ttggtggtgt tccggttaaa
      241 aatataggca taagggaggt gtacagcttt cttgaaatca gagcagctgg gagtaaattt
      301 gctattgcta accagtacag ggcgctttta tca
//
LOCUS       DAZPEB010000508          333 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.515, whole genome shotgun sequence.
ACCESSION   DAZPEB010000508 DAZPEB010000000
VERSION     DAZPEB010000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.515"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            42..242
                     /locus_tag="ACHURJ_004485"
     CDS             42..242
                     /locus_tag="ACHURJ_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFR1283110.1"
                     /translation="MKVKALEGDTVDSLCFRYYGTTQGVTEKVLDANPGLCQQVFLDA
                     GQEVEMPEPEKKKREMIQLWGE"
     gene            245..>333
                     /locus_tag="ACHURJ_004486"
     CDS             245..>333
                     /locus_tag="ACHURJ_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HFR1283111.1"
                     /translation="MSTIQTGITEQVIAWLFDHLPTVYAVGAAV"
BASE COUNT           78 a           77 c          113 g           65 t
ORIGIN      
        1 gacattctgt gccgcccgcg aatctttgcg gagttgtgct gatgaaagtg aaggcactgg
       61 aaggcgacac cgtggattcg ctctgtttcc ggtactacgg cacgacgcag ggcgtcaccg
      121 aaaaggtgct ggatgccaac cccggactct gtcagcaggt atttctggac gccgggcagg
      181 aagtggagat gccggagccg gagaagaaga aacgagaaat gattcagttg tggggggagt
      241 agcagtgagc accattcaaa cagggatcac agagcaggtt attgcgtggc tctttgacca
      301 cctgccaacg gtgtatgcag taggcgcggc ggt
//
LOCUS       DAZPEB010000509          330 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.516, whole genome shotgun sequence.
ACCESSION   DAZPEB010000509 DAZPEB010000000
VERSION     DAZPEB010000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.516"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(198..330)
                     /locus_tag="ACHURJ_004487"
                     /pseudo
     CDS             complement(198..330)
                     /locus_tag="ACHURJ_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
BASE COUNT           98 a           68 c           58 g          106 t
ORIGIN      
        1 attttcatat agtatgcaag cttctttgta tactatgatt taaaaaaacg gaacaaaaat
       61 gcaattatat tctggcattg tttaatgcgg tttgtctttt ttattcatta tttcaatgaa
      121 agaatttcaa ctacaaagtt aattgatcct acctacataa tatagacaca gtacctaaag
      181 taccgtggag atcatccggg gaagccccgg gtctgatacc agtgcaggta gaccgctatc
      241 ttgttccggt aatgttctgc cgttctttac ggcatcacct ctagcagaaa catcgcgtaa
      301 ctgcgccaag tatgatgaac ttctcttacc
//
LOCUS       DAZPEB010000510          329 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.517, whole genome shotgun sequence.
ACCESSION   DAZPEB010000510 DAZPEB010000000
VERSION     DAZPEB010000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.517"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..>329)
                     /gene="ykgH"
                     /locus_tag="ACHURJ_004488"
     CDS             complement(<1..>329)
                     /gene="ykgH"
                     /locus_tag="ACHURJ_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgH"
                     /protein_id="HFR1283112.1"
                     /translation="LLFLYINKDNIKVIYSLKINQTTPGILVSCDSNNNFACQTTMTE
                     DVIQRITTFFHTSPDVKNREIKLEWSGDKRDLPTAEAEISRVQASIIKWYASEYHNGR
                     QVLDEIQ"
BASE COUNT           85 a           66 c           59 g          119 t
ORIGIN      
        1 atttgtatct catcgagaac ttgcctgcca ttatggtatt ctgacgcata ccatttgata
       61 atagacgcct gcacgcgaga tatttccgct tcagcagttg gtaaatctct cttatctcct
      121 gaccattcca gctttatttc tctgttcttg acatctgggc tggtgtgaaa aaatgtagta
      181 attcgctgta taacatcttc agtcattgtg gtctgacagg caaaattatt attgctatca
      241 cagctaacaa gtatacctgg cgttgtctgg tttattttta ggctgtaaat cacttttata
      301 ttgtctttat tgatatacag aaacaacag
//
LOCUS       DAZPEB010000511          326 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.518, whole genome shotgun sequence.
ACCESSION   DAZPEB010000511 DAZPEB010000000
VERSION     DAZPEB010000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.518"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..>326
                     /locus_tag="ACHURJ_004489"
     CDS             <1..>326
                     /locus_tag="ACHURJ_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFR1283113.1"
                     /translation="EYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSSEACML
                     GGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVELREIPMRPGQLFMD
                     PKRMIEA"
BASE COUNT           74 a           85 c           97 g           70 t
ORIGIN      
        1 gaatatccgc aatccgcagc catcgacctg cgttgcgtaa atatggttgc cgatctgtgg
       61 catgcgcctg cgccgaaaaa tggtcaggcc gttggcacca acaccattgg ttcttccgag
      121 gcctgtatgc tcggcgggat ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca
      181 ggcaaaccaa cggataaacc aaacctggtg tgcggtccgg tacaaatctg ctggcataaa
      241 ttcgcccgct actgggatgt ggagctgcgt gagatcccta tgcgccccgg tcagttgttt
      301 atggacccga aacgcatgat tgaagc
//
LOCUS       DAZPEB010000512          325 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.519, whole genome shotgun sequence.
ACCESSION   DAZPEB010000512 DAZPEB010000000
VERSION     DAZPEB010000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.519"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..128
                     /gene="ospE2"
                     /locus_tag="ACHURJ_004490"
     CDS             <1..128
                     /gene="ospE2"
                     /locus_tag="ACHURJ_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 3 secretion system effector OspE2"
                     /protein_id="HFR1283114.1"
                     /translation="LQIPATNRTKTLKFTATEMQWLTKINEVGIVEKQSQRHSNI"
BASE COUNT          117 a           58 c           57 g           93 t
ORIGIN      
        1 aattacaaat acctgctacc aaccggacga aaacgttgaa attcactgct acagaaatgc
       61 aatggttgac aaagataaat gaagtaggaa ttgttgaaaa acaaagccaa cggcattcta
      121 atatctgaag ttaatttcag actcgtttaa gctccatttt caaaagaata tatcagggtt
      181 tgttactaat aatttctgga aacagaaaaa gtagagcagt aattctattt cagagaggag
      241 taactccatc tatcctcgat acatactgaa cgttatcgtg atatattcat catgctttct
      301 tcaccattat tcgagtgcag ggacg
//
LOCUS       DAZPEB010000513          323 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.520, whole genome shotgun sequence.
ACCESSION   DAZPEB010000513 DAZPEB010000000
VERSION     DAZPEB010000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.520"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..>323
                     /locus_tag="ACHURJ_004491"
     CDS             <1..>323
                     /locus_tag="ACHURJ_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFR1283115.1"
                     /translation="KQTMQLSRLTLRSKKPELVEQELWGVLLAYNLVRYQMIKMAEHL
                     KGYWPNQLSFSESCGMVMRMLMTLQGASPGRIPELMRDLASMGQLVKLPTRRGRAFPR
                     VVKERP"
BASE COUNT           94 a           58 c          103 g           68 t
ORIGIN      
        1 aaacagacga tgcaactgag caggctgacg ctgagaagta aaaagccgga gcttgtggag
       61 caagagctgt ggggtgtctt actggcttat aatctggtga gatatcagat gattaaaatg
      121 gcggaacatc tgaaaggtta ctggccgaat caactgagtt tctcagaatc atgcggaatg
      181 gtgatgagaa tgctgatgac attgcagggc gcttcaccgg gacgtatacc ggagctgatg
      241 cgcgatcttg caagtatggg acaacttgtg aaattaccga cgagaagggg aagggccttc
      301 ccgagagtgg taaaggagag gcc
//
LOCUS       DAZPEB010000514          322 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.521, whole genome shotgun sequence.
ACCESSION   DAZPEB010000514 DAZPEB010000000
VERSION     DAZPEB010000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.521"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..>322)
                     /locus_tag="ACHURJ_004492"
     CDS             complement(<1..>322)
                     /locus_tag="ACHURJ_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFR1283116.1"
                     /translation="IDGLPHVDFSRVDKFFTYADAFRESLPIISGELYFEAHQGCFTS
                     ESATKAHNRNMENKLHDAEFFTTITNNMTSILRSEFDEIWKEMLTLQFHDILPGSCIS
                     RVYLE"
BASE COUNT          107 a           58 c           61 g           96 t
ORIGIN      
        1 tttcaagata aactcttgaa atacatgatc cgggaaggat gtcatggaat tgcaaggtca
       61 gcatctcttt ccatatttca tcaaactcac tgcgaagaat tgatgtcata ttattagtaa
      121 tagttgtaaa aaattcagca tcatgtaatt tattttccat atttcgatta tgagctttag
      181 ttgctgactc tgatgtaaag cacccttgat gagcctcgaa ataaagttca cctgaaataa
      241 tgggcaagct ttcgcgaaaa gcgtcggcgt aagtgaagaa cttatcaaca cggctgaaat
      301 caacatgagg taagccatca at
//
LOCUS       DAZPEB010000515          319 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.522, whole genome shotgun sequence.
ACCESSION   DAZPEB010000515 DAZPEB010000000
VERSION     DAZPEB010000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.522"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..>319
                     /locus_tag="ACHURJ_004493"
     CDS             <1..>319
                     /locus_tag="ACHURJ_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFR1283117.1"
                     /translation="RVLGPRLEHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRL
                     GKRLAADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALEVEQRVLAHP
                     LYPV"
BASE COUNT           68 a          102 c           85 g           64 t
ORIGIN      
        1 agcgcgttct cggtccgaga cttgagcacc cggcggtact cgatcttctc cagcgatatc
       61 cctcaccaga aaaactcgct tcgctgggtg agaagaagct ggcagcccag ctctgcaaac
      121 ttgcgcctcg tctgggtaaa cgccttgcag cagacatagc tcaggcactg gccgaacaaa
      181 ccgtcgtcgt tcccggcacg aatgccgctg ccgtagtact gccacgtctg gcactccagc
      241 tcatcacgct gcgtaagcaa agagacgagg tggcgcttga ggtagaacag cgagttcttg
      301 ctcaccctct ttacccggt
//
LOCUS       DAZPEB010000516          318 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.523, whole genome shotgun sequence.
ACCESSION   DAZPEB010000516 DAZPEB010000000
VERSION     DAZPEB010000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.523"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..>318
                     /locus_tag="ACHURJ_004494"
     CDS             <1..>318
                     /locus_tag="ACHURJ_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR1283118.1"
                     /translation="SFSPDSRLTMKALEMAWETRGKPGGVMFHSDQGSHYTSRQFRQL
                     LWRYQIRQSMSRRGNCWDNSPMERFFRSLKNEWMPVVGYVSFSEAAHAITDYIVGYYS
                     ALRP"
BASE COUNT           82 a           76 c           99 g           61 t
ORIGIN      
        1 gtcgttctcg ccggacagca ggctcaccat gaaagcgctg gaaatggcat gggaaacccg
       61 tggtaagccc ggcggggtga tgttccacag cgatcagggc agtcattata cgagcaggca
      121 gttccggcag ttattgtggc gataccagat cagacagagt atgagccggc gcggaaactg
      181 ctgggataac agcccaatgg aacgcttctt caggagtctg aagaacgaat ggatgccggt
      241 ggtgggttac gtaagcttca gcgaggcagc tcacgccata acggactata tcgttggata
      301 ttacagcgca ctaagacc
//
LOCUS       DAZPEB010000517          314 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.524, whole genome shotgun sequence.
ACCESSION   DAZPEB010000517 DAZPEB010000000
VERSION     DAZPEB010000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.524"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..>314
                     /locus_tag="ACHURJ_004495"
     CDS             <1..>314
                     /locus_tag="ACHURJ_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HFR1283119.1"
                     /translation="PALVGNDIGCGMALWQTDILARKYNADKFEKRLSDLDDVAEESW
                     LEENLPSAFAQHPWCSSLGSIGGGNHFAELQQVDQIINAELFALAGLDAQHLQLLVHS
                     GS"
BASE COUNT           80 a           75 c           83 g           76 t
ORIGIN      
        1 atccggcact ggtcggcaat gatatcggct gcggtatggc gctatggcaa acagatattc
       61 tcgctcgcaa atacaacgcc gataagtttg aaaagcgatt atcagatctg gatgacgttg
      121 ctgaagaaag ctggctggag gaaaacctgc cgtcagcatt tgcacagcat ccgtggtgca
      181 gctcgcttgg ctctatcggt ggcggcaatc atttcgctga actgcaacaa gttgatcaaa
      241 tcatcaacgc tgaactgttt gcactagcag gtctggatgc acaacatctg caactgttgg
      301 ttcatagcgg ctcg
//
LOCUS       DAZPEB010000518          311 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.525, whole genome shotgun sequence.
ACCESSION   DAZPEB010000518 DAZPEB010000000
VERSION     DAZPEB010000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.525"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(1..311)
                     /locus_tag="ACHURJ_004496"
                     /pseudo
     CDS             complement(1..311)
                     /locus_tag="ACHURJ_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418674.2"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
BASE COUNT          106 a           47 c           49 g          109 t
ORIGIN      
        1 tttttatagg gttctttatc gagattgaaa aataaggtct cagctatttt aattttagat
       61 tgatcgcagc taaaatcttt agattgatcg cggctaaaat caatatgatt tatcacggag
      121 catgcatact ttatcttttc agaatcacca aaatattgca ttcttttatt aaaacaccaa
      181 tccagtattc ttacatcatt aatatatggg gattttaaaa aagtcatcca gaatcgatga
      241 ccggtgtaag gttaactttt gcatcatcta aggaatcaat aagaatatga gacaggttaa
      301 ctttgatgtc a
//
LOCUS       DAZPEB010000519          310 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.526, whole genome shotgun sequence.
ACCESSION   DAZPEB010000519 DAZPEB010000000
VERSION     DAZPEB010000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.526"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            13..>310
                     /locus_tag="ACHURJ_004497"
     CDS             13..>310
                     /locus_tag="ACHURJ_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1283120.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSG"
BASE COUNT          122 a           40 c           59 g           89 t
ORIGIN      
        1 tagacgctat atatgggtag taatatacat ggaattagtt gcactgcaaa taattatttg
       61 aaacaggcct ggaacgatat aaaaaatgag tacgaaaaaa atcaaacata ttcaatcacg
      121 ctttttgaaa acacactggt gtgttttatg cggttataca atgaactcag acgtaaagta
      181 aatgaagagg atactccatg tctggaatgt gaatcactag aaaaagaatt tgaggaaatg
      241 cagaatgata atgatctatc attatttatg agaacattgc gtactaatga tacacaaatt
      301 tattcagggt
//
LOCUS       DAZPEB010000520          309 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.527, whole genome shotgun sequence.
ACCESSION   DAZPEB010000520 DAZPEB010000000
VERSION     DAZPEB010000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.527"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..246
                     /locus_tag="ACHURJ_004498"
     CDS             <1..246
                     /locus_tag="ACHURJ_004498"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1283121.1"
                     /translation="CFFSAPPWTNFYSKKTRFVTACAQDISQQSATGKKNDCLVIFDS
                     GKRRFKRKQNIFCVDNSVFTWVLILWYEQKKKNTTRG"
BASE COUNT          107 a           54 c           64 g           84 t
ORIGIN      
        1 tgttttttct ccgcacctcc gtggacaaat ttttactcca aaaagacgag gtttgtcacg
       61 gcttgtgcgc aagacatatc gcagcaatca gcgacgggca agaaaaatga ctgtctggtg
      121 atttttgata gcggaaaacg gagatttaaa agaaaacaaa atattttttg cgtagataac
      181 agcgtattta cgtgggtttt aatactttgg tatgaacaaa aaaagaaaaa tacaacgcgc
      241 gggtgagtta ttaaaaatat ttccgcagac atactttcca tcataacgca gcgttaacaa
      301 aatacaggt
//
LOCUS       DAZPEB010000521          308 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.528, whole genome shotgun sequence.
ACCESSION   DAZPEB010000521 DAZPEB010000000
VERSION     DAZPEB010000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.528"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
BASE COUNT          107 a           63 c           49 g           89 t
ORIGIN      
        1 tatactttac aaaaagtttg tattagtgga ataaggacaa tagcttatcc aaactgccaa
       61 gcctctttat tataagtaag atctggcact ggaatattct caaaaggact aacatcatca
      121 ccacgacctt caaccaacca ttttattgca tttaaatccc agaatagttc agtagtggtc
      181 aatttgggaa acgcacaata ttgtacggtt acgttatcgc aatagagtga actcccgccc
      241 tatacggcgt tttcaaggat tagtccttaa tcggacaacc acattaaagc ataataaata
      301 attaagct
//
LOCUS       DAZPEB010000522          305 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.denovo.529, whole genome shotgun sequence.
ACCESSION   DAZPEB010000522 DAZPEB010000000
VERSION     DAZPEB010000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.denovo.529"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            <1..>305
                     /locus_tag="ACHURJ_004499"
     CDS             <1..>305
                     /locus_tag="ACHURJ_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFR1283122.1"
                     /translation="ALLNTVRAVLNAEDTRPVADRLTVQSARIVTWRLNAKLYFYPGP
                     ESEPILAAAESSFRKWLAEQGLIGQDVALSAIAAALHVHGVQRVEIIEPTQNMAISD"
BASE COUNT           64 a           87 c           91 g           63 t
ORIGIN      
        1 gcgttgctta ataccgttcg cgccgttctg aatgcagagg atacccgccc ggtggccgac
       61 cgcctgaccg tacagagtgc cagaatcgtg acatggcggc tgaatgcaaa actgtacttt
      121 taccccggcc cggaatccga acctattctg gccgcggctg aatcgtcgtt caggaagtgg
      181 ctggctgagc aggggcttat cggtcaggac gtggcgttgt ccgccattgc tgccgcactg
      241 catgtgcacg gtgtgcaacg cgtggagata atcgaaccca cacagaatat ggccatcagc
      301 gacat
//
LOCUS       DAZPEB010000523        27094 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.guided.033, whole genome shotgun sequence.
ACCESSION   DAZPEB010000523 DAZPEB010000000
VERSION     DAZPEB010000523.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27094
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.guided.033"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            50..523
                     /gene="dfrA1"
                     /locus_tag="ACHURJ_004500"
     CDS             50..523
                     /gene="dfrA1"
                     /locus_tag="ACHURJ_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HFR1283123.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            618..1142
                     /gene="sat2"
                     /locus_tag="ACHURJ_004501"
     CDS             618..1142
                     /gene="sat2"
                     /locus_tag="ACHURJ_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HFR1283124.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            1200..2035
                     /gene="aadA1"
                     /locus_tag="ACHURJ_004502"
                     /pseudo
     CDS             1200..2035
                     /gene="aadA1"
                     /locus_tag="ACHURJ_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
     gene            2064..2561
                     /locus_tag="ACHURJ_004503"
     CDS             2064..2561
                     /locus_tag="ACHURJ_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1283125.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            2622..2993
                     /locus_tag="ACHURJ_004504"
     CDS             2622..2993
                     /locus_tag="ACHURJ_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1283126.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            3403..3780
                     /locus_tag="ACHURJ_004505"
     CDS             3403..3780
                     /locus_tag="ACHURJ_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1283127.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            3803..3955
                     /locus_tag="ACHURJ_004506"
                     /pseudo
     CDS             3803..3955
                     /locus_tag="ACHURJ_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(4011..5627)
                     /locus_tag="ACHURJ_004507"
     CDS             complement(4011..5627)
                     /locus_tag="ACHURJ_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn7-like element transposition protein TnsE"
                     /protein_id="HFR1283128.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALEQSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(5628..7154)
                     /locus_tag="ACHURJ_004508"
     CDS             complement(5628..7154)
                     /locus_tag="ACHURJ_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsD family Tn7-like transposition protein"
                     /protein_id="HFR1283129.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQG
                     AVHLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVF
                     FDRAVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQW
                     TLFYQRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFR
                     KHRKAFSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVK
                     HKDWQQLVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDW
                     NQRDRIAVRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKR
                     YSESVEDYQIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE
                     "
     gene            complement(7157..8824)
                     /locus_tag="ACHURJ_004509"
     CDS             complement(7157..8824)
                     /locus_tag="ACHURJ_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR1283130.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            complement(8821..10929)
                     /locus_tag="ACHURJ_004510"
     CDS             complement(8821..10929)
                     /locus_tag="ACHURJ_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR1283131.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            complement(10916..11737)
                     /locus_tag="ACHURJ_004511"
     CDS             complement(10916..11737)
                     /locus_tag="ACHURJ_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsA endonuclease N-terminal domain-containing
                     protein"
                     /protein_id="HFR1283132.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            complement(11897..13726)
                     /gene="glmS"
                     /locus_tag="ACHURJ_004512"
     CDS             complement(11897..13726)
                     /gene="glmS"
                     /locus_tag="ACHURJ_004512"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFR1283133.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIMVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(13888..15258)
                     /gene="glmU"
                     /locus_tag="ACHURJ_004513"
     CDS             complement(13888..15258)
                     /gene="glmU"
                     /locus_tag="ACHURJ_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFR1283134.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILISNGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDVNLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(15609..16028)
                     /locus_tag="ACHURJ_004514"
     CDS             complement(15609..16028)
                     /locus_tag="ACHURJ_004514"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFR1283135.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQSGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(16049..17431)
                     /gene="atpD"
                     /locus_tag="ACHURJ_004515"
     CDS             complement(16049..17431)
                     /gene="atpD"
                     /locus_tag="ACHURJ_004515"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFR1283136.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(17458..18321)
                     /gene="atpG"
                     /locus_tag="ACHURJ_004516"
     CDS             complement(17458..18321)
                     /gene="atpG"
                     /locus_tag="ACHURJ_004516"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFR1283137.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHLYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(18372..19913)
                     /gene="atpA"
                     /locus_tag="ACHURJ_004517"
     CDS             complement(18372..19913)
                     /gene="atpA"
                     /locus_tag="ACHURJ_004517"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFR1283138.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(19926..20459)
                     /gene="atpH"
                     /locus_tag="ACHURJ_004518"
     CDS             complement(19926..20459)
                     /gene="atpH"
                     /locus_tag="ACHURJ_004518"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFR1283139.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(20474..20944)
                     /gene="atpF"
                     /locus_tag="ACHURJ_004519"
     CDS             complement(20474..20944)
                     /gene="atpF"
                     /locus_tag="ACHURJ_004519"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFR1283140.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(21006..21245)
                     /gene="atpE"
                     /locus_tag="ACHURJ_004520"
     CDS             complement(21006..21245)
                     /gene="atpE"
                     /locus_tag="ACHURJ_004520"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFR1283141.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(21292..22107)
                     /gene="atpB"
                     /locus_tag="ACHURJ_004521"
     CDS             complement(21292..22107)
                     /gene="atpB"
                     /locus_tag="ACHURJ_004521"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFR1283142.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(22116..22496)
                     /gene="atpI"
                     /locus_tag="ACHURJ_004522"
     CDS             complement(22116..22496)
                     /gene="atpI"
                     /locus_tag="ACHURJ_004522"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFR1283143.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(23113..23736)
                     /gene="rsmG"
                     /locus_tag="ACHURJ_004523"
     CDS             complement(23113..23736)
                     /gene="rsmG"
                     /locus_tag="ACHURJ_004523"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFR1283144.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(23800..25689)
                     /gene="mnmG"
                     /locus_tag="ACHURJ_004524"
     CDS             complement(23800..25689)
                     /gene="mnmG"
                     /locus_tag="ACHURJ_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFR1283145.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(26068..26511)
                     /gene="mioC"
                     /locus_tag="ACHURJ_004525"
     CDS             complement(26068..26511)
                     /gene="mioC"
                     /locus_tag="ACHURJ_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFR1283146.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(26601..27059)
                     /gene="asnC"
                     /locus_tag="ACHURJ_004526"
     CDS             complement(26601..27059)
                     /gene="asnC"
                     /locus_tag="ACHURJ_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFR1283147.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQHPIMRTIKP"
BASE COUNT         6912 a         6937 c         6240 g         7005 t
ORIGIN      
        1 acaaaacgag catgcttact aataaaatgt taacctctga ggaagaattg tgaaactatc
       61 actaatggta gctatatcga agaatggagt tatcgggaat ggccctgata ttccatggag
      121 tgccaaaggt gaacagctcc tgtttaaagc tattacctat aaccaatggc tgttggttgg
      181 acgcaagact tttgaatcaa tgggagcatt acccaaccga aagtatgcgg tcgtaacacg
      241 ttcaagtttt acatctgaca atgagaacgt attgatcttt ccatcaatta aagatgcttt
      301 aaccaaccta aagaaaataa cggatcatgt cattgtttca ggtggtgggg agatatacaa
      361 aagcctgatc gatcaagtag atacactaca tatatctaca atagacatcg agccggaagg
      421 tgatgtttac tttcctgaaa tccccagcaa ttttaggcca gtttttaccc aagacttcgc
      481 ctctaacata aattatagtt accaaatctg gcaaaagggt taacaagtgg cagcaacgga
      541 ttcgcaaacc tgtcacgcct tttgtaccaa aagccgcgcc aggtttgcga tccgctgtgc
      601 caggcgttag gcgtcatatg aagatttcgg tgatccctga gcaggtggcg gaaacattgg
      661 atgctgagaa ccatttcatt gttcgtgaag tgttcgatgt gcacctatcc gaccaaggct
      721 ttgaactatc taccagaagt gtgagcccct accggaagga ttacatctcg gatgatgact
      781 ctgatgaaga ctctgcttgc tatggcgcat tcatcgacca agagcttgtc gggaagattg
      841 aactcaactc aacatggaac gatctagcct ctatcgaaca cattgttgtg tcgcacacgc
      901 accgaggcaa aggagtcgcg cacagtctca tcgaatttgc gaaaaagtgg gcactaagca
      961 gacagctcct tggcatacga ttagagacac aaacgaacaa tgtacctgcc tgcaatttgt
     1021 acgcaaaatg tggctttact ctcggcggca ttgacctgtt cacgtataaa actagacctc
     1081 aagtctcgaa cgaaacagcg atgtactggt actggttctc gggagcacag gatgacgcct
     1141 aacaattcat tcaagccgac accgcttcgc ggcgcggctt aattcaggag ttaaacatca
     1201 tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg
     1261 agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg
     1321 gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa
     1381 caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg
     1441 agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt
     1501 atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta
     1561 tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac
     1621 atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg
     1681 atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg
     1741 gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca
     1801 aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc
     1861 agcccgtcat acttgaagct aggcaggctt atcttggaca agaagatcgc ttggcctcgc
     1921 gcgcagatca gttggaagaa tttgttcact acgtgaaagg cgagatcacc aaggtagtcg
     1981 gcaaataatg tctaacaatt cgttcaagcc gacgccgctt cgcggcgcgg cttaactcaa
     2041 gcgttagaga gctggggaag actatgcgcg atctgttgaa ggtggttcta agcctcgtac
     2101 ttgcgatggc atcggggcag gcacttgctg acctgccaat tgttttagtg gatgaagctc
     2161 gtcttcccta tgactactcc ccatccaact acgacatttc tccaagcaac tacgacaact
     2221 ccataagcaa ttacgacaat agtccatcaa attacgacaa ctctgagagc aactacgata
     2281 atagttcatc caattacgac aatagtcgca acggaaatcg taggcttata tatagcgcaa
     2341 atgggtctcg cactttcgcc ggctactacg tcattgccaa caatgggaca acgaacttct
     2401 tttccacatc tggcaaaagg atgttctaca ccccaaaagg ggggcgcggc gtctatggcg
     2461 gcaaagatgg gagcttctgc ggggcattgg tcgtcataaa tggccaattt tcgcttgccc
     2521 tgacagataa cggcctgaag atcatgtatc taagcaacta gcctgctctc taataaaatg
     2581 ttaggcctca acatctagtc gcaagctgag gggaaccact agtgtcatac gaacctccaa
     2641 gagacggtta cacaaacggg tacattgttg atgtcatgta tgacaatcgc ccaagtaagt
     2701 atccagctgt gttcagaacg tacgtccgaa ttccagacta tgtcgaacta gataacgcgg
     2761 aggagctggc cgacgcaatc attcgagagg ttgagttaaa ggaggcatgg ctcacaaggg
     2821 tcttggggaa ctcagccgat cctgacgctt ctgtgggcaa gtgggaccgg cgcctagcgc
     2881 agtgcggtgg tattgatgag atgtcaagtg gggtgcaggc aacatacttc agtccaaacg
     2941 gtcagctggt cggcttagtg gcatacattg atgccgaatg gatggatgaa tagctctgac
     3001 gttacgattc aggtgagaca accagatggg cattgcgtac aagctcagtt gccaccgatg
     3061 tggctacgag tctgatcttc tttacctagg ccaaggtatg gcgatgcttc ctgagcaaat
     3121 agtcggaacg tgcacatgct gcgacaatct aacgacgatt tctataaatg aaagctacgg
     3181 agtatgctca ctttgtgggg ctaaggatcg tgtgatattt acaagctatc agcatgaggc
     3241 aaggccaaga aatccctgct atctagagag aagtttcaaa tatcaatgcc caagatgcca
     3301 tgccttcagt atggagcctc cggatctacc ggagatgctt tgggactaat gctccgcttt
     3361 gcctcgggta tccgttcgac ctatgtttaa gaggaaacgc acgtgtctgc cgccaaagct
     3421 cttgcctatg cactttgtga caagttggaa agcaaaaaag ttgatgaagt cgtacgaaat
     3481 cttgtttcta gcggctggaa tattagggaa gtggcctggg aagcttcgtc ctttgatcat
     3541 gaaatgccct actttgacac ggggcgggac ttcgatattg gatttcctga cacgctgtca
     3601 tccaagattg agtttttgac cgcgaatgga tcaatcgtcc aagcacgtgc tcgcctgcac
     3661 ttcaagcagg ctctaatttt tgccaaggcg tccaaacatg tcgccgcgct caagaactca
     3721 ttggattact actatggatc gtctgtaatc ccccctcaaa accacggagg ctatttgtag
     3781 aaaattccat taatatgaac tcaaggtgtt ccctacggac gaatcagcga tgaaggtggt
     3841 gtacctggct atccaggcgg cgaccaagaa atggacgatg ccgatccgca actggaagcc
     3901 ggccatgaac cgttttatga tcgaatttgg tgaccgttta aatggccacc tttaactgac
     3961 cggcaaaagc acttacacaa aagggtttac aggctctttc agtcattcct ttaatgcgta
     4021 aattgctctc taaccactct ctcagcccac ccttccatat cctctctcga tacttgcagc
     4081 tcccccaacc cctttggatg attcacccct ctatgcccgt cttgtccata taattgatca
     4141 aacaaactat ttggccaatt caatgagctc ttcaccactc cacgccgtat cttttcaaaa
     4201 tcattccgcc atgtttcgct atcaacgcca ctcaacactt tggtagaaag cattttcacc
     4261 ccatccgatg catccacctc tagcaataca aattcactgc cattacgttg caccccaacg
     4321 gctttaatca ctcttggtga gccatcttta cacagatgta atcggctacg cccaactttt
     4381 ggcaaaacca aggtttcttc aaaaagcacc cgacatgcaa atttagtttt tagaattgaa
     4441 agtagctcat caaaagcagc aaatcgatta gcaaaaatag agttgtaatt ggttgcatcc
     4501 tgcttcccac ccacatctgc cgctgctagg acaccaccta agtgtggctc atcagtacta
     4561 accaaattac tattttcact tgttgttgct tcttcttttt ctaaaatagt ttggctactt
     4621 tttcttgccc tgcgagattt ttcaattcga ctaggtctac taaaacttat ccatgtcggc
     4681 tcagcctcta tgactcgtgt gtctgtgtct atattggctg tttcctcatc gtccaactgt
     4741 agatgctcat catcaacaac tgactctgtt gaaaccgcta tgtgctgcgt actaccatca
     4801 cctgcctcct tttcctgaaa agaggcatgg ctaatagctg ttgtgctagg aagcatagcg
     4861 ttgatttcta aacctactat ttcctcaact aaataatcct tatcctcgtt agaagaacgt
     4921 ccttttacat gtaatttcca accctgcatg ggcggagggt caaagctaaa gcaccagctt
     4981 tcaacgccgt tcttaatttc tctattttgt tgatagtgcc taaaaatact ttcatacgaa
     5041 tccataacat cttgatccga aaacaaccaa accaaaagct gcactacggc cgactgctct
     5101 aacgcccctt taggaaacga tgaactgggt aagatcctta tctctaaatg atctcgctca
     5161 acctcatact gaacgtcgaa ctcttgctgt aaagcggtac tgctcaaaca gcttcgacag
     5221 aaataggagt taattaaaaa taatgaccga gctagctcta actgtgggat gtgataacga
     5281 gtcttgctat atggtgtttc agcttcgtaa ataaatgaac tctgcgctcc atccttgttg
     5341 cgtacggaag caaaggacga gagattagga aaatcgatta ttttggctcg ttgaagatcc
     5401 gatgcttcaa actcaatcac acgatccgcc ttatttattt gctttgttgt actgttaacc
     5461 accttagctc gacttaataa aggcaaatga gtaaatcgag tccatttccg ttcttgcatt
     5521 ggattaaacc aaacaaaaat acgccactcc ttatgacccg agttacggaa taaatgacca
     5581 atatgtacaa cctgcacatt gtcattaaat gtagctagcc taaccactca ctcttcccca
     5641 taaaccattt ccaagaatct ttgtgcttcc tcagttatcc gctctttaga taacgttgca
     5701 cttcttaata atcgccagcg cctaagctca acatcttcct gtttaagctt aataaaagct
     5761 tggctaatcc gtctaatttg ataatcttcc acactctctg agtaacgctt taagcaaagc
     5821 gctaccaaag gcagtttctg tagatttttt gcaagagagg ttccgttagg agtttgcttt
     5881 aacagccagc tcgatgtcgc tcttgggtga tcaaggctac tatctagacg ctttatgatt
     5941 cttaatagtt gtcgtacagc aattcgatct ctttggttcc aatcaactct aggggcgggt
     6001 gccagacgct cttgttgatg ctgttgattc cagtgaacta gccaatccct gtcatgtcgg
     6061 taaagccaag cgtatagcac cccaccctct aaagactgtc ttgccgcctt aattccttgg
     6121 tatttatgca ctagttgctg ccagtcttta tgcttaacag ataaatcttc agagttaggt
     6181 tgcacagact ggctaacagg tcttgtcgtt atagagtgct cagtaagagc acttgcctgc
     6241 tgtagcgctt ctataaccgt tagttttggc aataaggctt gccacacaat actatgctgt
     6301 aaataactaa aggcttttct atgcttacgg aatatacttt tcagccaaca cgtgtccttg
     6361 ttctctgcca actttaaatc cagtttctct agtgcctcat cactaaaagt ctgcctcact
     6421 ctctccgcca ccaagtcatg acgaatgtgc ttgcttttgg ttagccctag atcctgcgct
     6481 aagcgctgat aaaacagcgt ccactgctca aggcttgggg aaagctcttg cgctcgtgga
     6541 gcatctaaca gaggggctat ataagcagct agtgctgtta attgagatag ggagtctttg
     6601 gggtagtctg aaagcagctc agtatgaccc aaagcccaaa attgatgtcg gtgatcatct
     6661 acagctctat caaagaagac taaagcaccg tgttttggac aatatggcaa agcgggcaaa
     6721 taccaatctc gttgccaaaa ggcttcccca tacctattta gctgaagagc aacgcaatca
     6781 gggcagtatc taaagcggtt atcgctctta actctagaag cagcgactcc tagcattaaa
     6841 tgcaccgcac cttgcgcttg gtactccatt aaccgaatag cttcgtctcg gcgctccttg
     6901 cctacaaacg gagcatataa agggaataag gtatgctcat aaataagctg ctgaacagcg
     6961 taacgtcctg tttgatgtag atgtcttgct atcacaccta aatgcgaggg cagacctaag
     7021 gtagcgacca ccttgcggtt gccatacacc tcatccaaca gctgcttagg actaacaatc
     7081 ccttgataaa cgcctgcccg tgcaatagtg ctataaatca gctcattcga gtacggaaca
     7141 ggaaagtttc tcatggctaa cctgcttgtt taaataagct cgccatatcc actattaccc
     7201 ctttcccttt taaccgttca tgcatggttt tttcaggttg gcgttgtgaa tagatataac
     7261 gtaaatccgt atcaggcaag ctatcccagt cgctgggctt gactaccttt atagccgaaa
     7321 cctttttact cttggagggc ttttctagtt ctgacactac cgtttcgcct tccatcaacc
     7381 actgcaaaac aagaggcagt aacttttgtc ttgtcatcgt tggattctgg ctaaacgctt
     7441 ttttaatagt gggaattaac aggcttgaat cgtaatcctc tttcagcatc agatataaat
     7501 gacgctgatc ttcggtatct aactcttgaa gggctttttc ttctggtgtt tgttcttgta
     7561 tcgctgcgat atctagctga agttggatta accgtttatc aatctcggga acgactagat
     7621 cagaataacg agcaatgcgt tctgggatac ccgagcgtaa tgcctctagc atggggtgca
     7681 caggctttaa ctcatcttga tacacttgcc gcaataaacc agcggtaata cgctcattgc
     7741 ctaaagctag cgcacggagc tgagcgagta caaaaagttt tactacaatg tccatcactc
     7801 cttggcttag ctcataccac acatcacgga cctcatccga taacagcgca tctttgcgtt
     7861 gtaaaagctg taattgccaa agattatccg taaaagcgat ccactcttga ttgggctttc
     7921 cacgttgcgt ttgttgtata ggatcccaga atatagctcc aaaccctgcc cctctacgtg
     7981 cagaccgcaa atcagcctca aaaatctctc gtgctttagg ggtaccaatc aacatcactg
     8041 gtacgccaat aatattcacc atcgtcacaa aaaagttcag catctcttga gatccacccg
     8101 aacgagagcg gcttaaatgc tgaatttcat caataaccaa caaccctaaa gcatgtgcat
     8161 tggctatttg cgacatcaaa gccaacatgg tttctatacc atgacgtttt aagccataac
     8221 gacgctcata gttcgagccc aaggctcgat ccaacgctct gaaaaaattc aagcagattt
     8281 cttttagcga accattatgc gagcagtcta ttttcaaata caccacctgc tctacattga
     8341 gttcacgatg gtaaatcacc tgaggatacg tggctagaat acgatgaaga gaggtcgtct
     8401 tcccactacc agaacaacca attaacaata agctttgtgc cgtagatcgt gcctcctcaa
     8461 agcgaaatgt ctccaactct cccgtttgaa cacgctcata accattttgt aaatgctttt
     8521 gtaaatctcc tgttttagga tttctgccta cgtagccacc tcgaatcatg accgaaatac
     8581 gctcacttag tagcaaatgc gtacctaatg gctgaaaata gtcatctgga atacgacaaa
     8641 tggtatgagc tctgataaca cgggactttt gcaagtcaga ggaagtaagc tgtaaagagg
     8701 atttcagtga tgcagcacta ttcactgact cttgtaatgg tggtaaggcc tcgataaaag
     8761 gattatcacg ataagcctct acccccgtat cacgataaac tgcttgaatc cgggtagcac
     8821 tcatgactca tccttttctg gtggatcctg aaataattca ggcacgtatg tgggtaggct
     8881 gtaatcttct tgatcatccg cttccactgc gttgaaagga ataactttag cctcatcacc
     8941 tgaagagctt ggcttcaaat gctccgcacg ttttttacgc tccgaggtca cggcttcttt
     9001 tttattagtt ttaatctgct taatgcgtgt tgatttgggc tcagtagtac tgggcgttaa
     9061 cttattcgct ttctgaatgg tttgctgaat aaacgcctca agctccctgc gtttagttaa
     9121 ctcatcctgc ttcgcattgg ctttattgtg tttttcttgt gcttgtatat cccaaacctc
     9181 ccaaaatgag agacctttaa actgccgact acgttccgtc agattacagc gccaaaatac
     9241 acggctgcca acttgcggaa acaaataaat cgtatcaacc agcactgggt cataagccgc
     9301 ttctaaatgt tgaggtctag ctatatcagt gctccgctgc aaccaaccct cacgcagaat
     9361 ctctgacccc gagtaataca aaccccacaa attaacgcca aatgaagaaa tagagacctt
     9421 tcggcgaggc agtaacgcta ctcgcaactg ctcttgctcc acagccctta aactacctgt
     9481 acgatgctgc ataccccatt gccatagctg gacaggaata gacggtaaat ctgtaggaaa
     9541 atcagcatct cgatcgtatt tatccatcac cagatggtta tttctgaata agatcgtacg
     9601 caaaataatt tgtgtgaact caaataccga cagagatgca tctaacctat agtctgtttc
     9661 accatggctt ttgatccgac tgccctctac aatgccaggt gcaaaggact taaactcggc
     9721 ttgtagtgtt ctaaaagtgc tttccactat gcctttagca tcgccacgtc taggtggagc
     9781 actttccact cgcacattaa aactagaaac taaggcttcg acctgatgac tcattaattc
     9841 gccacggtcc gctagcaaca catctggcaa acctacacac ggccagtctg agctactaat
     9901 ctcaatatca tgctgggcac aaatggccgt tttgtcagag caagcattta caaaagcctg
     9961 catcgccacc acataagacg gattttcaaa gccgatataa aagcccgtga tcatccgact
    10021 aaacacatca atcacaatgt aaagcgttgg tcttcctatg attttttggc gatcatgatg
    10081 atccactaaa taaatatcag caatcgtggc atcaatctca taacgactcc cagggcctaa
    10141 cgcctgagaa gtggctgtac tacttaaggg tcgtacgtct tttttatata cccctgcttt
    10201 aactctagac tttaagcgct gagctttagg gtattctcga tcataaaaat aacgaaactg
    10261 acgtagtgtt gggtaatcct cttggggaat gcgaggaaaa tactgagcaa acaagtccac
    10321 aaatcgtcta taggcaacgg tggtctttgt acctttttga tttaacaggt gcttttctat
    10381 ggtcaaccta aaaaggcgtt caatctcggg cgttaccttg gttccttcac ccttaccata
    10441 ttctctggct cggccaatct ttgctgttcc tgtcgctgaa cgtctttccc ctggtgcacc
    10501 gctgtttttg tagtcaggaa ttaatgcatt aggcgtttga ccacgctgcc agtaacggcg
    10561 taacaactta taaactgtag ccttagtaac cttatgttct tggaccacat gctcaacgag
    10621 ttcgcttctg actttagggt cgaaacgatc cttactatta ataataggaa gaatttttcg
    10681 ataatcctcc tcgcgcttct ggaagctgac agaatctaca gacggctctt ctaaatcaag
    10741 atgtacataa gggtcatcag ttctaactaa gcggccttca tctaaatact gcattagcaa
    10801 caactcagcc ctagcttgtg gaactccttt atcagcgctt atttgcatcc agacaacttg
    10861 gccatcctct atagccaaaa tgcgatacgg atcattatca aatagcacaa cctcattaat
    10921 ttgccacata gcgcaactcc tccatattca ctacttggct aatacagaga tctgcacact
    10981 tatttgccct gaaagactta taaatattga acttaataaa accatttgcg gttaaggctc
    11041 gtatctcact caatgttttg cctaactcca aatcataagc aatatcaacc tgcttacaga
    11101 cattgataat gttttcatct cctttttctt gcaggatatg ggccaatggg gatagttgtg
    11161 ctaaaagctc cgcagaaact tcttctgttt tcactgaata aagccattca atattttctt
    11221 ttactacggg atttatttct ttatcagtaa aaatgaacca aggaatttgc ttttgctgcc
    11281 aatagcgacg ctctagttct agtttttcta aggtacgctc gtcttgtaag gctgctgcag
    11341 gtttgacttg aatagcaaac tgctcaaaag gaccatcttt gcagtccact aaaaaatcag
    11401 tagacataac ctgatctaca ccacgaataa caggatgctt aataccacta tctattgcaa
    11461 tctgcctggt atcactaggt aataagggga actgctcgcg tatatctagc acgctgctct
    11521 cccactcaag actgagaaaa acagcaagct ctaagtcaga tagcaaatga tggactcgtc
    11581 ccgtcttatg agaataaata cggtgggaac gacctgaaga aggaacttct tgtactgtta
    11641 gccatggaat atagtcttta ccatgccctt ggccacgccc ctctttaata cgacgggcaa
    11701 tttgcacttc agaaaatgaa gagtttgctt tagccataac aaaagtccag tatgcttttt
    11761 cacagcataa ctggactgat ttcagtttac aactattctg tctagtttaa gactttattg
    11821 tcatagttta gatctatttt gttcagttta agactttatt gtccgcccac aagccctttt
    11881 ttatctatga tagtgattac tcaaccgtaa ccgattttgc caagttacgc ggctggtcaa
    11941 cgtcggtgcc tttgatcagc gcgacatggt aagccagcag ctgcagcgga acggtgtaga
    12001 agatcggtgc aatcacctct tccacatgcg gcatctcgat gatgtgcatg ttatcgctac
    12061 ttacaaaacc cgcatcctga tcggcgaaga catacaactg accgccacgc gcgcgaactt
    12121 cttcaatgtt ggatttcagt ttttccaaca gttcgttgtt cggtgcaacg acgataaccg
    12181 gcatatccgc gtcaatcagc gccagcgggc cgtgtttcag ctcgccagca gcgtaggctt
    12241 cagcgtgaat gtaggagatc tctttcagct tcaatgcgcc ttccagtgcg attgggtact
    12301 gatcgccacg gcccaggaac agtgcgtgat gtttgtcaga gaaatcttct gccagagctt
    12361 caatgcgttt gtcctgagat agcatctgct caatacggct cggcagcgcc tgcagaccat
    12421 gcacaatgtc atgttcaatg gaggcatcca gacctttcag gcgagacagc ttcgccacca
    12481 gcatcaacag cacagttaac tgagtggtga atgctttagt ggacgccacg ccgatttccg
    12541 tacccgcgtt ggtcatcagc gccagatcgg attcgcgcac cagagaagaa cccggaacgt
    12601 tacagattgc cagcgaacca aggtaaccca gctctttcga cagacgcagg cccgccaggg
    12661 tatccgcggt ttcgccagac tgtgacaagg tgatcatcag gctgttacga cgcacggcag
    12721 atttacgata gcggaattca gaggcgattt cgacgtcgca cggaatacct gccagcgatt
    12781 caaaccagta gcgggaaacc ataccggagt tataagaagt accacaggcg aggatctgaa
    12841 tatgctcaac cttcgacagc agttcgtcgg cgttcggtcc cagctcgctt aaatcaacct
    12901 gaccgtggct gatgcgccca gtaagggtgt ttttgatcgc gttcggctgt tcgtagatct
    12961 ctttctgcat gtagtgacgg taaatacctt tatcgcccgc gtcatattgc agattggatt
    13021 cgatatcctg acgttttact tccgcgccag ttttatcgaa gatgtttacc gaacggcgag
    13081 taatttccgc aatatcgccc tcttcaagga agataaagcg acgggtcacc ggcagcagcg
    13141 ccagctggtc agaagcgata aagttttcgc ccatacccag gccaatcacc agcggactac
    13201 cagaacgtgc cgccagcagg gtatccgggt gacgggagtc catgatcact gtaccgtacg
    13261 caccgcgcag ctgcgggata gcacgcagaa cagcctcacg cagagtcccg ccttgtttta
    13321 gctcccagtt caccagatgg gcaatcactt cggtgtcggt ttcagaaacg aaggtatagc
    13381 cacgcgcttt taactcttca cgcagcggtt catggttttc gatgatgccg ttatgcacca
    13441 ccataatgtg ttcagaaaca tgcggatgcg cgttagcttc tgaaggttca ccgtgggtcg
    13501 cccagcgagt gtgagcaata ccggtgccgc catgcagagg atgttcttcc gctgcctgag
    13561 ccagcatctg gactttaccg aggcgacgca ggcgggtcat atgaccttcc gcatcaacaa
    13621 cggccagacc agcagagtca tatccgcggt attccagacg acgtaaacct tcaagaagga
    13681 tttctgctac atcacgttgc gcgatcgcgc caacaattcc acacatagtt tttgattccg
    13741 atttatatcg ttgtcggtca acctgtatgc ccgttttttc gggcgccccg agccttgtag
    13801 agagtggggt tatttttata gttactgctt gtgggaggga gattatgtta tctcctcatc
    13861 ccagatgacc ggattacccg gccagaatta ctttttcttt accggacgac gccagccttc
    13921 tttctgagtc tgcggcacac ggctgatagc taatgcattt tcgccgacat tacgcgtcac
    13981 agttgtaccc gcagcaatgg tcgcgccttt gcctactgtt accggggcca ccagctgagt
    14041 gtcggaaccg acaaacacat cgtcgccgat aatggtctta aatttattcg caccatcgta
    14101 gttgcaggta atggttcccg cgccgatgtt aacgttatcg ccaatttccg catcgcccag
    14161 gtaagtcaga tgaccagctt tcgagccttt acccagacgc gcttttttca tctcaacaaa
    14221 gttaccgacg tgtgcacctt ccagcaactc agcaccagga cgcagacggg caaacgggcc
    14281 aatagtacag gccgctgcca gattcacgtc ttccacaacg gtatacggac tgatttcgca
    14341 atcatcgcca atcacgctgt ttttaatcac gcaaccggtg ccgattttca cgcgatgacc
    14401 gagagtcacg ttgccctcga tgataacgtt agtatcaatt tcaacatcgc gcccgtgagt
    14461 aagcgtaccg cgcagatcaa aacgcgccgg atcgcgcagc ataacgcctg ctaacagcag
    14521 tttttcagcc tgttcggact ggtaaacgcg ctccagacgg gagagttgca ggcggttatt
    14581 cacaccttct acttcgctta aacgttgcgg atgaacggcg acgatttcac gcccttcctg
    14641 atacgccagc gcaataatgt cggtgatgta gtattcgccc tgagcattat tgttggtcag
    14701 cttcgccagc cagcgtttca tatctgcgcc gttagagatc agaatgccgg tgttgatctc
    14761 ctgaatctga cgctgctcgt cggtggcatc tttgtgctca acaatgccgg taactttgcc
    14821 attttcacgg gtgatacgtc cataaccggt cggatcatcc agtttcaccg tcagcagacc
    14881 aatgccaccc tgcggtttag catcacgcag gcgctggagt gtttcgacag agatcagcgg
    14941 cacgtcgccg tagagcatta aaatgtcttc atcatcggca aagaaaggtg cggcctgctg
    15001 cattgcatga cccgtaccca gctgctctgc ctgaagcacc cagttaaggt tgtcgtcttt
    15061 cagcgcctgt ttaagcagat cgccgccgtg accgtacacc aggtgaacgt gcgctgcgcc
    15121 taattcattc gcagcatcaa tgacatgctg aaccatcgct ttcccggcaa gggtatgcag
    15181 cactttcgga agatcggaat acatgcgcgt gcctttgcct gcggcaagga tcactacgct
    15241 catagcatta ttcaacatac gcgtcctgac tgtaatttga gaacgaattt aaaccgcttc
    15301 accttgaaaa aactacattt ttttcatcgt gaaatggaca gaggataaat tgttcaataa
    15361 cggattatcc ccgctgacga aagtgccatt ttcgaccatc gtcacgcctt ttgtctgcag
    15421 aaaattaagg cagtaccttc atttaagcgt caggtggatg tttttgctct tatttcgatc
    15481 aatgaataaa cgggaaaaca ggggtgtttt atatcagcag gatctatgtg aacgctcttc
    15541 aggacggatc acgcgtaaaa aaaaaccagc ctgtttccag actggctttt gtgcttttca
    15601 agccggtgtt acatcgcttt tttggtcaac tcgataacgc gcagctgcgc gatcgctttg
    15661 gccagttccg cagacgcctg agcgtaatct acgtcgccgt gagagctgct aatgtgctct
    15721 tcagccttac gtttcgcttc catggctcgc gcttcgtcga gatcctggcc gcgaattgcg
    15781 gtgtcggcca gaacggtcac gttgccagac tgcacttcaa gaatgccgcc agacagatag
    15841 ataaactctt cgtgaccgtg ctgtttcacg atgcgaatca taccaggctt aatggcggtg
    15901 agcagcggtg cgtggccagg gtagatcccc agttcacctt cgctacccgt tacctggatt
    15961 ttctcgacca gaccagagaa catttgttgc tctgcgctga cgacgtccag gtggtaagtc
    16021 attgccatat caccctccga ttaaggcgtt aaagtttttt ggctttttcc acagcttctt
    16081 cgatggaacc gaccatgtag aacgcctgct ccggcaggtg atcgtattcg ccttccatga
    16141 tgcctttaaa gccacggatg gtgtctttca gggagacgta tttacccgga gaaccggtga
    16201 atacttctgc cacgaagaac ggctgggaca ggaagcgctg gatcttacga gcacgcgcta
    16261 ccaccagttt gtcttcttca gacagttcat ccatacccag gatggcgatg atgtctttca
    16321 gttcctgata acgttgcagg atggactgaa cgccacgcgc ggtgtcgtag tgttcctgac
    16381 caaccaccag cgggtccagc tgacggctgg tggagtccag cgggtcaacg gccgggtaga
    16441 tacccagaga cgcgatctga cggctcagta ccacggttgc gtcaaggtgc gcaaaggtgg
    16501 ttgccggaga cgggtcagtc aagtcatccg caggtacgta taccgcctgt acggaggtga
    16561 tagaaccagt tttggtggag gtgatacgtt cctgcagaac gcccatctct tccgccaggg
    16621 tcggctgata acctaccgct gaaggcatac ggcccagcag tgcggatact tccgtaccgg
    16681 ccagggtgta acgatagatg ttgtcaacga acagcagaac gtcacgacct tcgtcacgga
    16741 atttctcagc catggtcaga ccggtcagag caacgcgcag acggtttccc ggcggctcgt
    16801 tcatctggcc atacaccagg gatactttgt cgataacgtt ggagtcggtc atttcgtggt
    16861 agaagtcgtt accctcacga gtacgttcac ctacgcccgc aaacacagag taaccggagt
    16921 gctcgatcgc gatgttacga atgagctcca tcatgtttac ggttttacct acacccgcac
    16981 caccgaacag accaacttta ccgcccttag cgaacggaca catcaggtcg ataactttga
    17041 taccggtttc cagcagttcc tgagagtttg acagctcttc gtaggaaggt gctgcgcggt
    17101 gaatcgccca acgctcttct tcaccgatct cgcctttcat gtcgaccggt tcacccagta
    17161 cgttcatgat acggcccaga gtcgctttac ctaccgggac ttcgatcggg tgttcgaggt
    17221 cttttacatc cagaccgcga cgcagaccgt cggaggaacc cattgcgatg gtacgcacga
    17281 taccgccgcc gagctgctgc tgaacttcca gcaccagacg ctcattacca ttttgcacct
    17341 caagagcatc gtacacgcgc ggtacggcat cctgagggaa ttcgacgtca actacggcgc
    17401 cgattacctg gacaatcttt ccagtagcca tcttaaatcc tctacgaaat aacctgttta
    17461 aaccgcggcg gcccccgaga cgatctcggt gagttcctga gtaatgctgg cctgacgagc
    17521 tttgttgtat accaactgca gctctttaat caggctgccg ccattgtcgg tcgcggcttt
    17581 catcgccacc atacgggcgg cctgctcgct ggccaggttt tcaaccacgc cctgataaac
    17641 ctgagattcg acataacgac gcagcagggt atccagcaac gccttcggat cgggttcgta
    17701 caggtaatcc caggatttat gtttcagatc atcatcatct gatgccggta acggcagcag
    17761 ctggctgatg gtcggaacct gagacatggt gttaataaat ttgttgctga caatgtaaag
    17821 cttgtccaga cggccttcgt cgtaggcctg caacatcact tttaccggac cgatcagttc
    17881 ggacagggaa gggttatccc ccatgccggt gacctgggca acaacattgc cgcccacgga
    17941 gttgaagaac gacacgcctt tcgagccgat cattgcgagg tcgcattgaa cgcctttgtc
    18001 ggtccaggtc ttcatttccg ccagcagttt tttgaacagg ttaatgttca aaccaccgca
    18061 caaaccacgg tcggtcgaca ccaccaggta gcccacgcgt ttaacgtcgc ggtcttccag
    18121 gtaaaggtgc ttatattcca gattaccatg tgcaaggtga ccaatcactt tgcgcatggt
    18181 ttctgcataa ggacggctgg ccgccatgcg atcctgcgat ttacgcattt tggaagcggc
    18241 gaccatctcc atcgctttag tgatcttttg cgtgttctgg acgcttgcga tcttactacg
    18301 tatctctttt gcgccggcca tgagcttctc ctcaatgcct tgcggcctgc cctaaggcaa
    18361 gccgccagac gttaccagga ttgggttgct ttgaaggaat cgaggatgcc tttcagtttg
    18421 ccttcgattt cgtcgttgta gccaccggtc tggttgatct cttgcatcaa cggagcgtga
    18481 tcacggtcga cgtaagccag cagagcggct tcgaagctgc caattttcga cagttcaaca
    18541 tccgccaggt aaccacgttc tgctgcgaac agaaccagag actgctgcgc aacggacatc
    18601 ggcgcatact gtttctgttt cagcagttcg gtcactttct gaccgtggtc aagctgctta
    18661 cgtgttgcat cgtcaaggtc ggatgcaaac tgagagaacg ctgccagttc acgatactgt
    18721 gccagagcgg tacggatacc accggacagt tttttcatga tcttggtctg tgctgcacca
    18781 ccaacacggg atacggaaat acccgggtta accgcaggac gaataccggc gttgaacagg
    18841 ttggtttcca ggaagatctg accatcggta atggagatta cgttggtcgg aacgaacgca
    18901 gaaacgtcac ccgcctgagt ttcgataatc ggcagtgcgg tcagagaacc ggttttccct
    18961 ttcacttcac ctttggtgaa ggcttcaacg tattcggcgt taacacgtgc agcacgctcc
    19021 agcagacgag agtggaggta gaaaacgtcg cccgggaatg cttcacgtcc tggcggacga
    19081 cggagcagca gggagatctg acggtaagca acagcctgtt tagacaggtc atcgtaaatg
    19141 atcagcgcat cttcaccgcg gtcacggaag tattcgccca ttgcgcaacc ggcatacggt
    19201 gccaggtatt gcagtgcagc ggattcagac gcggttgcta ccacaacgat ggtgttagcc
    19261 agtgcgccgt gctcttccag tttacgtacc acgttagaaa tggtggacgc tttctggccg
    19321 atagcgacat agatacattt gataccggaa tcgcgctggt tgatgatggc atcgatagcc
    19381 agtgcggttt tacctgtctg acggtcaccg atgatcaatt cacgctgacc acgaccgatt
    19441 gggatcatgg agtcaacggc tttataaccg gtctgtaccg gctgatctac ggactgacgt
    19501 tcgataacgc ccggagcgat tgcttctaca gcagagaagc cgtcgtgatc cagcggacct
    19561 ttaccgtcga ttggtgcacc cagagtgtta accacacggc ccagcaggcc acggccaacc
    19621 ggaacttcca ggatacggcc agtacactta actttcatgc cttcggcaag gtcagcgtac
    19681 ggacccataa caaccgcacc tacagagtcg cgctcgaggt tcagtgcgat agcgtaacgg
    19741 ttacccggca gggagatcat ttcaccctgc atacaatcgg ccaggccgtg aatgcggata
    19801 acaccgtcac ttacagaaac aatagtacct tcgttgtgag cttcactcac aacattgaac
    19861 tgagcaatgc gctgcttgat cagttcgctg atttcggtgg aattcagttg catgctccag
    19921 tccccttaag actgcaagac gtctgcaagg cgctcaagac gaccgcgtac gctgccatca
    19981 atgaccatat cacccgctcg gatgataacg cctgccatta cagacttatc gattttgcaa
    20041 ttcagcttaa ctttgcgtga cagacgtttt tccatcgcag cagaaatttt cgcgagctgt
    20101 tgttcactca gtgcggcagc ggaaatgacg tctacctcag cggtagcctc actcacggca
    20161 cgaaggtgaa taaactgctc cagaacatcc gggagcgcgt taagacgacc attttcagcc
    20221 attacccgaa tcaggttctg accgttttcg tccagttgct caccacaaac tgcgataaac
    20281 gactcggcga gcgtttctgg cgcaagcgcg ccagagagaa gctctgccat ttgttcgttt
    20341 ttggttacct cggcggcaaa cgccagcatg tcctgccagc gttctacact ttggtgttcg
    20401 acggcaaagt caaaagctgc tttggcgtag gggcgagcta ccgtaataaa ttcagacatc
    20461 agcccctccc tccttacagt tcagcgacaa gtttatccac gatgtcgctg ttagcagctt
    20521 catccacgga acgttcgatg atcttctcgg cgccagcaac agccaggata gcaacttgct
    20581 tacgcagctc ttcacgggca cgtttacgct cggcttcaat ttctgcctgc gcctgggcca
    20641 cgattttagt acgttcctgt tctgcctcag ctttcgcttc gtccagaatc tgcgagcggc
    20701 gtttgttcgc ctgctcgatg attacctggg cttccgcttt cgcttttttc agctggtcgg
    20761 tcgcgctggc ctttgcaagg tcaaggtcct tatgtgctcg ttctgcggaa gcaaggccgt
    20821 cagcaatttc tttttgacgt ttttcgatgg ctgccattaa tggcggccat acgtacttca
    20881 tgcagaacag aacgaacagg acaaacgcga tggcctggcc gaggattgtt gcgttaagat
    20941 tcacagcaca atgcctctat ttagttaacg ttctgatatt gctctttaaa taaaagcaac
    21001 gcttactacg cgacagcgaa catcacgtac agacccagac ctacagcgat catcgggata
    21061 gcatccacca gacccataac gataaagaac tgagtacgca gcagaggaat cagatcaggt
    21121 tgacgcgctg cgccttccaa gaatttaccc ccgaggatgc cgataccgat cgcagcaccg
    21181 attgccgcca gacccatcat cacagcggca gccatgtaca gcagatccat attcaggttt
    21241 tccatgacag tctccagttt gtttcagtta aaacgtagta gtgttggtaa attaatgttc
    21301 ttcagacgcc atcgacagat agacgatcgt cagaaccatg aagatgaagg cttgcagcgt
    21361 aatgatcagg atgtggaaaa tggcccacgg cacattcagg atccactgtg accaccacgg
    21421 caacagacca gcaatcagaa tgaaaatcag ctcaccggca tacatgttac cgaacagtcg
    21481 caaaccgagt gaaactggtt tggacagcag gcttacccct tcaaggatta agttgacagg
    21541 aatgaacgcc cagtgattga acggctgcag cgtcaactct ttcgtgaagc cgccgatgcc
    21601 tttcattttg atgctgtaga acagaatcag gataaatacg cccaatgcca tagacagcgt
    21661 tacgttcacg tccgcagacg gaaccacacg cagtgcaggc agacccagta catgttcagc
    21721 aatgtacggc agcaggtcga taggcagtaa atccatcagg ttcatcagga atacccagac
    21781 gaagatcgtc agggccagcg gagcaatcag cttgcttttg ccatggtaca tgtctttcac
    21841 gctaccatta acaaagccga tcaccagctc aatcgcggtc tgaaacttac ctggcacacc
    21901 gctggtcgcc tttttggcta cgctacggaa taaaaccagg aacaacagac ccagcaccac
    21961 cgagaagaac atggagtcaa tattgattgt ccagaaggtg gctggggggt tttgtggatc
    22021 caccagcgag aatgtacgca ggtccagctg aaggttattc aggtggtgtc ctatgtaatc
    22081 ctgcggcgtc atattttctg aagccatgat gccttttacc ctttgttgtt aattacagcc
    22141 ggtgccagta tctgaaccac cagcaccaaa acccacgtaa cgatcagcgg caagaatacc
    22201 gcctttaaaa ccgccaacgc caccaccagt aacaccaaca tcgccagaac tttgaaagct
    22261 tcgccaaatg cgaatgtcca ggccacccgg cctttcgctg gtgtatgcgc ctggtgacgc
    22321 caggcaaata tcataaacaa aacgttaggc agaaagactg ccaggccccc gcttattgca
    22381 gagacgcccc agaaggggtc tttgaggctg aacagcaatc cacttgctat caccaccagt
    22441 aactgaacga gcagaagctt ccgagcaacg tttcgactca cgagcgacac agacatcacg
    22501 ttttttactc ctgctccctt cgaggtatgc cgcgtgtcgt ataaaacttt ctttaaggct
    22561 tagagtcaag catcaaaaag cggtcaaatt atacggcgcg cccccgtgat ttcaaacaat
    22621 aagtagccaa aaggtgaata aatgtttaaa tatttttcca gtgcatacaa ttgcgacttt
    22681 tctactaacc ctgttcgatc atgaaaaact gtaaataacg cgtaaacact ggtgataaag
    22741 cgtgcttcag atcacatatt gcgcatgttc gcgcacagca tatttattta cttggcaaat
    22801 gatgcctttg caagtttatg atatttcagt ctaaaaacag atactgtttt aataaatgac
    22861 atttacacaa caaaaaccac ccattgacat ttttaataat gttttagcag ccaatgatgg
    22921 ttcttagcgc cgatttttag cagactgata ttttcactaa tgacttattt tctgcttacc
    22981 aaaaaaagcc acgttatctt gttgatgcaa aagagtgaac gtggcgttaa atgtaaccag
    23041 ttatatcagt agaaaacctg gttgttgtta acagtctaac cggtcaattt tttatgattt
    23101 ttttgataaa aattaaattt tatttgcttt aatcaccacc agatgacgtt cgccatccag
    23161 ggctggaacc tgaagtttaa ccactgattc gacctgatat tcttcgggca acaaagcgat
    23221 ttcatcttcc ggcatttgcc ctttcagcgc gtagaaacgg ccttgctcac caggaagatg
    23281 gtggcaccag ctcaccatat cgttcagaga ggcaaaagcg cggctaatta cgccatcaaa
    23341 tggcggctct gaaggaaact cttctaccct gctctgtaca ggttcaatat tctccagttt
    23401 aagctcatgt tgcacctgac gaaggaaacg cacgcgttta ccaaggctat ccaacagagt
    23461 gaaatgggct tcaggacgca cgatagagag tggaatgcct ggcagtcctg gtccggtgcc
    23521 gacatcgata aaccgttcac cttgcagata cggtgccacc acaatgctat cgagaatatg
    23581 gcgtaccagc atctcattag gatcgcggac cgaagtcagg ttgtacgctt tgttccattt
    23641 atgcagcata ttcacgtagg caataagctg gtttttctgg tgatcggtaa gcgaaatacc
    23701 tgcgtctttc agcagtaagg agagtttgtt gagcacggtg attacctgtt cttgatgcgt
    23761 tgcctggtaa gcgggtgctt accaggcatt tttaatgcgt tatgcgctac gacgcagcat
    23821 accctgtttt ttcagccaca ccagcagaat ggagatagcc gcaggcgtga cgccagaaat
    23881 acgcgaagct tggccgatag aggctggttt gtgatcgtta agtttggcga tcacttcgtt
    23941 agaaagaccg gatacctggc ggtaatccag tgtcgcgggt agcagggtgt tctcgttacg
    24001 cagctgcttt tcgatctcat cttgctggcg cgcgatataa ccttcgtatt taacctgaat
    24061 ctcaacctgt tccgccgcct gttcgtctgt caacgcaggg gcaaacggcg tcagcgtggt
    24121 taatttttca taagtcattt ccggacgacg cagcagatct tcaccactgg cttcacggga
    24181 aagcggcgca gtcaggtgag cattcacttc ggctgcagct tccgccgacg gggttaccca
    24241 ggtcgatttc agacgctgac gctcacgctc gatattctca agtttctcgt taaagcgcgc
    24301 ccaacgttcg tcatccacca ggcccagttc acgaccgatt tcagtcaaac gcagatccgc
    24361 attatcttcg cgtagcatca gacgatattc tgcgcgcgaa gtaaacatac gatacggttc
    24421 tttggttcct aaagtgcaca ggtcatcaac tagtacgccg agatacgcct gagaacgtgc
    24481 cggagcccaa ccttctttgt cagcagacag acgggcagcg ttaagaccgg ccagcaaacc
    24541 ttgcgcagcg gcttcttcgt aaccggtagt gccgttaatc tgaccagcaa agaacagccc
    24601 ctggataaac ttgctctcca gcgtcggttt caggtcgcga ggatcgaaga agtcatactc
    24661 aatggcataa cccggacgca cgatcttcgc gttttccatc ccctgcatag agcggacgat
    24721 ttgcatctgc acatcgaacg gcaggctggt ggagataccg ttcggataaa tttcattaga
    24781 ggtcagtcct tccggttcaa ggaagatctg atgctgattt ctgtcggcga agcgcatgac
    24841 tttgtcttcg atcgacgggc agtagcgtgg gccgacacct tcgatcaccc ctgcatacat
    24901 tgggctacga tcgaggttac tgcggatcac atcatgggtt ttctcgttgg tatgagtgat
    24961 ataacacggc acctgctggg gatgctggga cgcattgccc ataaacgaga ataccggcat
    25021 tgggttatcg ccatgctgtt gcgccagcac actaaagtcg atagttcgag catcaatacg
    25081 cggtggtgtc ccggttttca gacgaccaac gcgcagcggc agttcacgca aacggcgaga
    25141 aagcggaatg gacggcggat caccagcacg gccaccgctg taattatcca gaccgatatg
    25201 aattttaccg tcgaggaacg tcccaacggt gagcacgacg gctttggcac ggaacttcag
    25261 tcccatttgg gtaacagcac cgaccacgcg atcgttttcg acaataagat cttcaaccgc
    25321 ctgctggaag atcatcaggt tcggttggtt ctccagcgcc gtacgtaccg cctgacggta
    25381 gagcacacga tccgcctgag ctcgggtagc gcgaaccgcc ggtcctttgc ttgcgtttag
    25441 tatcctaaac tggatacccg cttgatcgat cgctttcgcc atcagaccgc cgagtgcatc
    25501 cacttctttt accagatgtc ccttcccaat accgccgatc gccgggttgc agctcatctg
    25561 ccccagagtg tcgatattgt gtgtcaaaag cagagtctgt tgacccatac gcgccgcggc
    25621 catcgcggcc tcggtgcctg catgaccccc gccaatgatg atgacgtcaa aaggatccgg
    25681 ataaaacatg gtgattgcct cgcataacgc ggtatgaaaa tggattgaag cccgggccgt
    25741 ggattctact caactttgtc ggcttgagaa agacctggga tcctgggtat taaaaaggag
    25801 atctatttat ttagagatct gttctattgt gatctcttat taggatcgca ctgccctgtg
    25861 gataacaagg atccggcttt taagatcaac aacctggaaa ggatcattaa ctgtgaatga
    25921 tcggtgatcc tggaccgtat aagctgggat cagaatgagg ggttatacac aactcaaaaa
    25981 ctgaacaacc gttgttcctt ggataactac cggttgaacc aagcttcctg acagagttat
    26041 ccacagtaga acgcacgatc tgtatactta tttgagtaaa ttaatccacg atcccagcca
    26101 ttcttctgcc ggatcttccg gaatgtcgtg atcaagaatg ttgatcttca gtgtttcgcc
    26161 tgtctgtttt gcaccggaat ttttgagttc tgcctcgagt ttatcgatag ccccacaaaa
    26221 ggtgtcatat tcacgactac caataccgat tgcgccaaag cggaccgcag aaagatcggg
    26281 cttctgttcc tgcaatgctt catagaaagg agaaaggttg tccggaatat ctccggcacc
    26341 gtgggtggag ctgataatca gccagatccc cgaggcagat aaatcttcca acagcggacc
    26401 gtgcagcgtt tcggtggtaa aacccgcctc ttccagcttt tcagccaggt gttctgctac
    26461 atattcggca ccgccgaggg tgctgccgct gataagagtg atatctgcca taaaccgcca
    26521 cctttattaa gagtggcgta ttgtacgctg tgaacgcgtt gggatctacc tgtggaaaag
    26581 tatgggatta aaaaagccga tcagggcttg atagtacgca tgatcgggtg ttgcaggacg
    26641 atcaatgtct cggtggactg aatttcatca attgtttgga tcttgttgat aagtacatgc
    26701 tggagagcgt cgatcgaacg gcacattact tttataaaga tgctgtagtg gcctgttgtg
    26761 taataggctt cagtgacttc atcaaggctt tccagctttg ccagcgcgga agggtagtct
    26821 ttggcgctct ttaatataat gccgataaag cagcctacgt cataaccgag ctgcttcggg
    26881 ctgacatcaa tacgcgcccc ggtaatgatc cccgcctgct tcattttctc tacccgaacg
    26941 tgaatcgtcc ccggactgac gccaaattgt ttcgccagtt cggcgtaagc ggtgcgcgca
    27001 ttgcccatta atgcttccag gatgccacgg tccagattgt cgatcagata attttccata
    27061 ggattttctt atgcagattg atgattcatt ctat
//
LOCUS       DAZPEB010000524        11188 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.guided.136, whole genome shotgun sequence.
ACCESSION   DAZPEB010000524 DAZPEB010000000
VERSION     DAZPEB010000524.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11188
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.guided.136"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(37..603)
                     /locus_tag="ACHURJ_004527"
                     /pseudo
     CDS             complement(37..603)
                     /locus_tag="ACHURJ_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            complement(822..1799)
                     /gene="epmA"
                     /locus_tag="ACHURJ_004528"
     CDS             complement(822..1799)
                     /gene="epmA"
                     /locus_tag="ACHURJ_004528"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFR1283148.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLNLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            2124..3932
                     /gene="frdA"
                     /locus_tag="ACHURJ_004529"
     CDS             2124..3932
                     /gene="frdA"
                     /locus_tag="ACHURJ_004529"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFR1283149.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRMAGEQATERAATAGNGNEEAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3925..4659
                     /gene="frdB"
                     /locus_tag="ACHURJ_004530"
     CDS             3925..4659
                     /gene="frdB"
                     /locus_tag="ACHURJ_004530"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFR1283150.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4670..5065
                     /gene="frdC"
                     /locus_tag="ACHURJ_004531"
     CDS             4670..5065
                     /gene="frdC"
                     /locus_tag="ACHURJ_004531"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFR1283151.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPDAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            5076..5435
                     /gene="frdD"
                     /locus_tag="ACHURJ_004532"
     CDS             5076..5435
                     /gene="frdD"
                     /locus_tag="ACHURJ_004532"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFR1283152.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            5498..6631
                     /locus_tag="ACHURJ_004533"
     CDS             5498..6631
                     /locus_tag="ACHURJ_004533"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFR1283153.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQMPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6720..7253
                     /gene="blc"
                     /locus_tag="ACHURJ_004534"
     CDS             6720..7253
                     /gene="blc"
                     /locus_tag="ACHURJ_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFR1283154.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDGGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGLFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(7250..7567)
                     /gene="sugE"
                     /locus_tag="ACHURJ_004535"
     CDS             complement(7250..7567)
                     /gene="sugE"
                     /locus_tag="ACHURJ_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFR1283155.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7749..7895)
                     /gene="ecnB"
                     /locus_tag="ACHURJ_004536"
     CDS             complement(7749..7895)
                     /gene="ecnB"
                     /locus_tag="ACHURJ_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFR1283156.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(8006..8131)
                     /gene="ecnA"
                     /locus_tag="ACHURJ_004537"
     CDS             complement(8006..8131)
                     /gene="ecnA"
                     /locus_tag="ACHURJ_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFR1283157.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(8183..8749)
                     /gene="efp"
                     /locus_tag="ACHURJ_004538"
     CDS             complement(8183..8749)
                     /gene="efp"
                     /locus_tag="ACHURJ_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFR1283158.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8791..9840
                     /gene="epmB"
                     /locus_tag="ACHURJ_004539"
     CDS             8791..9840
                     /gene="epmB"
                     /locus_tag="ACHURJ_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFR1283159.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEIDETFRQAMAKLRRVGVTLLNQSVLLRDVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQLLIKQGS"
     gene            10214..11083
                     /locus_tag="ACHURJ_004540"
     CDS             10214..11083
                     /locus_tag="ACHURJ_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFR1283160.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEVEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
BASE COUNT         2933 a         2828 c         2788 g         2639 t
ORIGIN      
        1 atcatacact aaatcagtaa gttggcagca ttacccgaaa tgcccaccat ttaataacaa
       61 aatagtaatg cttactaaca gcaatactaa attcagacac attactgcaa taccgcccac
      121 cgcagtaatg cgggcaattt tattaatccc ctttgaagca acgacggtga ccagaatcat
      181 ccatgctact gccagcagac caaccacctg cgtaggctcc agtccggcaa tacgccagtg
      241 ctgggtcatg tcgctaccat agaggaatgt tgagaacggt acccaaactt tcgcggaggt
      301 actcaccatc cagatgatat aagaggaaaa ccacataaac gtaccaatga aggcaaaacg
      361 tggtccgaca ctattattca tccaggaata gataccacct tcttctttgc gataagcagc
      421 tcccatttca gccatcatta aggcgaatgg aataaagaat aacaatgcag aaaatatata
      481 aaagggaatc gcactataac ccattaagta ataagccgat gggctattgg caaatccaaa
      541 tacggaagta aagatcatca atatgagtcc tatcagactc atctttttta tcgtgtgagg
      601 catgaaacca tccttcattt cagcgcgtgt ataacacgtg cccactgaac tgcactgcgc
      661 aacattgagc aggcagcgta aaaaccaata cagaattgat acccggatga tatcaaatat
      721 ccagtcttaa attgtggcct ttaacggaaa aatgaaaaca tatgctacag aatggcgctt
      781 atcacgccat tcttcgctgt taattcagta atttttcaga attatgcccg gtcaacgcta
      841 aaggcgatga cttcagccag tgtctccgcg cccagcgcca acatcaccag acgatcaaca
      901 cctaatgcca cgccggaaca gtcaggcata ccgactttca aggcttcaat cagattctgg
      961 tcaatggggt gctgcggcaa accgcgcgcc gcgcgcttac ggttatcttg ttcaaagcgt
     1021 tgttgctgct cacgggcatc cgttaattca tggaaaccat tcgccagctc aataccttta
     1081 taataaacct caaagcgttc agcgacccga tgatcttcgg tactgatttg cgccagtgat
     1141 gcctggctgg ctggaaagtg gtacacaaag gtcggttttt ctttgccaat atttggctct
     1201 acgccaaagg taaacagcaa ttgtagcaac gtgtcgcggt cttcttcggt atcagcaacg
     1261 ttactcaaat tcagtttcgc tgcgacttcc cgcagttgcg ttttgtcggc agagagcggg
     1321 tcaatttcca gataacgcaa gaaagcttgt tgataagaaa ggctttctgc tgccgggcag
     1381 tccagcacct gttgtaagag atcgtccacc tcgttcatca accggtacat atcatagtgc
     1441 ggtcgatacc actcaagcat agtgaactca gggttgtgat aacgccccat ctcttcatta
     1501 cggaagctgc ggcacagctg gaataccggc ccacaaccgg caaccagcag gcgtttcata
     1561 tggtattccg ggctggtcat taaccagaga ttcatcccct gcgaatgccc ggggccaacg
     1621 aaacgtgtct caaacgggac caaatgaata tcggttaccg tcgcctggct catacaaggc
     1681 gtctccacct ccagcactcc acgatcagca aagaaacgac ggatctccgc cataatcgcc
     1741 gcgcgtttta ataagttagg aatggatgcg ctcggctgcc aggatgccgt ttcgctcata
     1801 gttaaatctc cagtttttga caagggcacg aagtctactc gcaacgcgac ggcgagacaa
     1861 attttacgca ggaatcaaac agcggtgggc agtgactaaa aaaagcacga tctgatggtt
     1921 tagtaattaa attaatcatc ttcagtgata atttagccct cttgcgcact aaaaaaatcg
     1981 atctcgtcaa atttcagact tatccatcag actatactgt tgtacctata aaggagcagt
     2041 ggaatagcgt tcgcagaccg taactttcag gcacttaccc tgaagtacgg ggctgtggga
     2101 taaaaacaat ctggaggaat gtcgtgcaaa cctttcaagc cgatcttgcc attgtaggcg
     2161 ccggtggcgc gggattacgt gctgcaattg ctgccgcgca ggcaaatcca aatgcaaaaa
     2221 tcgcactaat ctcaaaagta tacccgatgc gtagccatac cgttgctgca gaagggggct
     2281 ccgccgctgt cgcgcaggat catgacagct tcgaatatca ctttcacgat acagtagcgg
     2341 gtggcgactg gttgtgtgag caggatgtcg tggattattt cgtccaccac tgcccaaccg
     2401 aaatgaccca actggaactg tgggggtgcc catggagccg tcgcccggat ggtagcgtca
     2461 acgtacgtcg cttcggcggc atgaaaatcg agcgcacctg gttcgccgcc gataagaccg
     2521 gcttccatat gctgcacacg ctgttccaga cctctctgca attcccgcag atccagcgtt
     2581 ttgacgaaca tttcgtgctg gatattctgg ttgatgatgg tcatgttcgc ggcctggtag
     2641 caatgaacat gatggaaggc acgctggtgc agatccgtgc taacgcggtc gttatggcta
     2701 ctggcggtgc gggtcgcgtt tatcgttaca acaccaacgg cggcatcgtt accggtgacg
     2761 gtatgggtat ggcgctaagc cacggcgttc cgctgcgtga catggaattc gttcagtatc
     2821 acccaaccgg tctgccaggt tccggtatcc tgatgaccga aggctgccgt ggtgaaggcg
     2881 gtattctggt caacaaaaat ggctaccgtt atctgcaaga ttacggcatg ggcccggaaa
     2941 ctccgctggg cgagccgaaa aacaaatata tggaactggg tccacgcgac aaagtttctc
     3001 aggccttctg gcacgaatgg cgtaaaggca acaccatctc cacgccgcgt ggcgatgtgg
     3061 tttatctcga cttgcgtcac ctcggcgaga aaaaactgca tgaacgtctg ccgttcatct
     3121 gcgaactggc gaaagcgtac gttggcgtcg atccggttaa agaaccgatt ccggtacgtc
     3181 cgaccgcaca ctacaccatg ggcggtatcg aaaccgatca gaactgtgaa acccgcatta
     3241 aaggtctgtt cgccgtgggt gaatgttcct ctgttggtct gcacggtgca aaccgtctgg
     3301 gctccaactc cctggcggaa ctggtggtct tcggccgtat ggccggtgaa caagcgacag
     3361 agcgtgcagc aactgccggt aatggcaacg aagaggcaat tgaagcgcag gcagctggcg
     3421 ttgaacaacg tctgaaagat ctggttaacc aggatggcgg cgaaaactgg gcgaagatcc
     3481 gcgacgaaat gggcctagca atggaagaag gttgcggtat ctaccgtacg ccggaactga
     3541 tgcagaaaac catcgacaag ctggcagagc tgcaggaacg cttcaagcgc gtgcgcatca
     3601 ccgacacctc cagcgtgttc aacaccgacc tgctctacac cattgaactg ggccacggtc
     3661 tgaacgttgc tgaatgtatg gcgcactccg caatggcacg taaagagtcc cgcggcgcac
     3721 accagcgtct ggacgaaggt tgcaccgagc gtgacgacgt caacttcctc aaacacaccc
     3781 tcgccttccg cgatgctgat ggcacgactc gcctggagta cagcgacgtg aagattacta
     3841 cgctgccgcc agctaaacgc gtttacggtg gcgaagcgga tgcagccgat aaggcggaag
     3901 cagccaataa gaaggagaag gcgaatggct gagatgaaaa acctgaaaat tgaggtggtg
     3961 cgctataacc cggaagtcga taccgcaccg catagcgcat tctatgaagt gccttatgac
     4021 gcaactacct cattactgga tgcgctgggc tacatcaaag acaacctggc accggacctg
     4081 agttaccgct ggtcctgccg tatggcgatt tgtggttcct gcggcatgat ggttaacaac
     4141 gtgccaaaac tggcatgtaa aaccttcctg cgtgattaca ccgacggtat gaaggttgaa
     4201 gcgttagcta acttcccgat tgaacgcgat ctggtggtcg atatgaccca cttcatcgaa
     4261 agtctggaag cgatcaaacc gtacatcatc ggcaactccc gcaccgcgga tcagggtact
     4321 aacatccaga ccccggcgca gatggcaaag tatcaccagt tctccggttg catcaactgt
     4381 ggtctgtgct acgccgcgtg cccgcagttt ggcctgaacc cagagttcat cggtccggct
     4441 gccattacgc tggcgcatcg ttataacgaa gatagccgcg accacggtaa gaaggagcgt
     4501 atggcgcagt tgaacagcca gaacggcgta tggagctgta ctttcgtggg ctattgctcc
     4561 gaagtctgcc cgaaacacgt cgatccggct gcggccattc agcagggcaa agtagaaagt
     4621 tcgaaagact ttcttatcgc gaccctgaaa ccacgctaag gagtgcaaca tgacgactaa
     4681 acgtaaaccg tatgtacggc caatgacgtc cacctggtgg aaaaaattgc cgttttatcg
     4741 cttttacatg ctgcgcgaag gcacggcggt tccggctgtg tggttcagca ttgaactgat
     4801 tttcgggctg tttgccctga aaaatggccc ggacgcctgg gcgggattcg tcgacttttt
     4861 acaaaacccg gttatcgtga tcattaacct gatcactctg gcggcagctc tgctgcacac
     4921 caaaacctgg tttgaactgg caccgaaagc ggccaatatc attgtaaaag acgaaaaaat
     4981 gggaccagag ccaattatca aaagtctctg ggcggtaact gtggttgcca ccatcgtaat
     5041 cctgtttgtt gccctgtact ggtaaggagc ctgagatgat taatccaaat ccaaagcgtt
     5101 ctgacgaacc ggtattctgg ggcctcttcg gggccggtgg tatgtggagc gcaattattg
     5161 cgccggtgat gatcctgctg gtgggtattc tgctgccact ggggctgttt ccgggtgatg
     5221 cgctgagcta cgagcgcgtt ctggcgttcg cgcagagctt cattggtcgc gtattcctgt
     5281 tcctgatgat cgttctgccg ctgtggtgtg gtttacaccg tatgcaccac gcgatgcacg
     5341 atctgaaaat tcacgtgcct gcgggcaaat gggttttcta cggtctggct gctatcctga
     5401 cagttgtcac gctgattggt atcgttacaa tctaacgtat cgccaatgta aatccggccc
     5461 gcctatggcg ggccgttttg tatggaaacc agaccttatg ttcaaaacga cgctctgcgc
     5521 cttattaatt accgcctctt gctccacatt tgctgcccct caacaaatca acgatattgt
     5581 gcatcgcaca attaccccgc ttatagagca acaaaagatc cccggtatgg cggtggcggt
     5641 aatttatcag ggtaaacctt attactttac ctggggctat gcggacatcg ccaaaaagca
     5701 gcccgtcaca cagcaaacgt tgtttgagtt aggttcggtc agcaaaacat ttacgggcgt
     5761 gcttggtggc gatgctattg ctcgagggga aatcaagtta agcgatcccg caacaaaata
     5821 ctggcctgaa cttaccgcta aacagtggaa tgggatcaca ctattacatc ttgcgaccta
     5881 caccgctggc ggcctgccat tgcagatgcc ggatgaagtg aaatcctcaa gcgacttgct
     5941 gcgcttctat caaaactggc agcctgcatg ggctccagga acacaacgtc tgtatgccaa
     6001 ctccagtatc ggtttgttcg gcgcactggc tgtgaagccg tctggtttga gttttgagca
     6061 ggcgatgcaa actcgtgtct tccagccact caaactcaac catacgtgga ttaatgtacc
     6121 gcccgcagaa gaaaagaatt acgcctgggg atatcgcgaa ggcaaggcag ttcatgtttc
     6181 gccaggggcg ttagatgctg aagcttatgg tgtgaagtcg accattgaag atatggcccg
     6241 ctgggtgcaa agcaatttaa ccccccttga tatcaatgag aaaacgcttc aacaagggat
     6301 acaactggca caatctcgct actggcaaac cggcgatatg tatcagggcc tgggctggga
     6361 aatgctggac tggccggtca atcctgacag catcattaac ggcagtgaca ataaaattgc
     6421 actggcagca cgcccggtaa aagcgattaa gcccccaact cctgcagtac gcgcatcatg
     6481 ggtacataaa acgggggcga ccggcggatt tggtagctat gtcgcgttta ttccagaaaa
     6541 agagctgggt atcgtgatgc tggctaacaa aaactatccc aatccagcga gagtcgccgc
     6601 cgcctgtcag attctcaacg ctctacagta aaatttcatc gggtccgaat tttcggacct
     6661 tttctgcgct tttccttgct gtcatctaca cttagaaaaa aaccagtaag gaaacattta
     6721 tgcgcctgct ccctctcgtt gccgcagcga cagctgcatt tctggtcgtt gcctgcagtt
     6781 ctcctacgcc gccgcgtggc gtgacagtag tgaataattt cgacgctaaa cgctatctcg
     6841 gtacctggta tgagattgcc cgttttgatc accgttttga acgtggactg gaaaaagtca
     6901 ccgcaacata cagtctgcgt gatggcggcg gcctgaatgt catcaataaa ggctataacc
     6961 ctgacagaga aatgtggcag cagagtgaag ggaaagcgta ctttaccggc gcaccaacac
     7021 gcgctgcgct gaaagtgtca ttctttggtc ttttctatgg cggttataac gtcattgcac
     7081 ttgatcggga ataccgccat gcgctggttt gcggcccgga ccgcgactac ctgtggatac
     7141 tctcccgcac gccaaccatt tctgacgaag tgaaacagga gatgctggca gtcgcgaccc
     7201 gggaagggtt tgatgtcagt aagtttattt gggtacagca gcccggtagt tagtgagtgc
     7261 tgagtttcag accaataatc cccaatacga ttaacgccag actcgccagg cgcatcgggt
     7321 tagcggactc accgagcagc acaatgccgg tgatagccgc gccgactgcg ccaatacccg
     7381 tccacacggc ataagccgtc cctactggta acgatttcat cgcccaggca agtagcgcca
     7441 tactgacaat catcgccgtc acggtaataa cactcggcgt caaacgacta aagccgtggg
     7501 tatatttcag gccaacggcc cataccactt ccagcagacc agcaataact aagataatcc
     7561 aggacatatc aggctccgga gaattggggc cgtccccggt gaaaagacgc gtttgcaggt
     7621 cgtcctgcaa agctgatggt tgaacggata ttttgccagg tggcagggga atttcaacta
     7681 ttaccttagt gactatcgca gaaaaaacaa acggcacgac acagctgtcg taccgttatt
     7741 gcttattgct attgctgcgc tttcgttgct gcgccagaaa tcgcgttacc gccatcagaa
     7801 atgtcttcac caacgccacg cgtggtgttg caggcagtta atactgttga aagcaccaga
     7861 acagaaaaga tcgctgcaat tgtcttcttc accataacgt cttcctttta ggtaatgtta
     7921 tttatgtttg cctatagcaa attaataata gacaacatcg ccaaaaatga cggaaatctg
     7981 atgatttttc ggaagagaag aaaaattagc tggcagcgcg agagatggag ttgccgagat
     8041 gtttgatgtc ttcgccgaaa ccgcgagcgg tgttacagcc cgtgagcagc gtactggcaa
     8101 gcaaaacaag aacgataagg cgtttcatca tctctgacct gaataagtga tggtgcagcc
     8161 tgcaggccgc accacaaccg cattacttca cgcgagagac gtattcacca gagcgggtat
     8221 ccactttgat gacttcgccg atttgtacga acagcggaac cttaaccaca gcgccagtag
     8281 acagggtagc cggtttgccg ccagtacctg cggtatcacc tttcaggccc ggatcggtat
     8341 caacgatttc cagttcaacg aagttcggcg gagtaacgga gatcggctga ccattccaca
     8401 gagttacgat acactctgcc tgatccagca gccatttagc gttatcgcca attgcttttg
     8461 catcagcaga cagctgctcg aaagtttcgt tgttcatgaa gtgccagaac tcaccgtcgt
     8521 tgtacaggta agtcaggttc atatcgacaa catcagcgcc ttcagcggaa tcagtagatt
     8581 tgaaggtttt ttctacgcga gtaccggtca gcagacgacg cagtttaacg cgagcaaatg
     8641 cctggccttt acccggtttt acgaattcac tcgcttcaac cgcgtaaggt tcgccgtcta
     8701 acatgatttt aagaccagca cgaaaatcgt tgctatagta cgttgccata aggccctctg
     8761 aaatttgtta attggtagct aagccacaaa atggcgcata ttgtaaccct aaatacccca
     8821 tccagagaag attggttaac gcaacttgcc gatgttgtga ccgatcctga tgaacttctg
     8881 cgtcttttga atatagacgc ggacgaaaaa ctgttggccg gacgcagcgc caaaaagctg
     8941 tttgccctgc gtgtgccccg ctcatttatc gatcgcatgg agaaaggcaa tccggacgat
     9001 cctcttttgc gtcaggtact tacctcgcaa gatgagtttg tcgtcgcgtc cggattctcc
     9061 accgacccac tggaagaaca gcacagcgta gtgcctggtt tgttgcataa ataccacaac
     9121 cgggcgcttt tgctggtcaa aggcggctgc gcggtaaatt gccgctattg cttccgtcgc
     9181 cacttcccct atgccgaaaa tcagggcaac aagcgtaact ggcagactgc acttgagtat
     9241 gttgctgcgc atccggaact ggacgagatg attttctccg gcggcgatcc gctgatggcg
     9301 aaagatcacg agctggactg gttgctcaca caactggaag ccatcccgca tatcaaacgt
     9361 ctgcggattc acagtcgtct gccgattgtg atcccggcac gcatcacaga tgcgctggtt
     9421 gaacgctttt cacattctac gctgcaaatc ctgctggtga atcacatcaa ccatgccaat
     9481 gagatagatg aaacattccg tcaggcgatg gctaagttgc gccgggtagg cgttactttg
     9541 ctgaaccaga gcgttctgtt acgtgatgtg aacgataacg cacaaacgct ggcaaacctg
     9601 agtaatgcgt tgttcgatgc cggcgtgatg ccgtattacc tgcatgtgct cgataaagta
     9661 cagggcgcag cgcattttat ggtgagtgat gacgaagcac ggcagattat gcgtgagttg
     9721 ctgacattgg tgtcgggata tctggtgccg aaactggcgc gagaaattgg cggcgaaccc
     9781 agcaaaacgc cgctggatct ccagctacgc cagcagttac tgattaaaca gggaagttaa
     9841 acattaaaat tgcctgatgc gctacgctta tcaggcctac atcgtgcatt gaaattgaat
     9901 ggatttgcaa tgaactgtag gccggataag gcgtttacgc cgcatccggc aagagcaaca
     9961 aacgcaagta cagcaacctg tcattaaact gagaaattta atctttgccc gttgcataac
    10021 ctttaattat tcatatgcat tgctgttgtt actttttata tagctaacac caataattaa
    10081 cctttctttt ctgtttatat agagtgagca cggctattta tgttatttta acaactgtta
    10141 ctaccaagcc catttatctg gcttataaat taaaattaat tatttaattg agaaatggtt
    10201 agggagaacc tacatggcca taagcatcaa gggtgttaat accggtgtta ttcgcaagag
    10261 taataatttc attgcactgg cgctgaaaat taaagaacct cgcaataaag aatcgttgtt
    10321 ctttatgtct gtcatggaac tgcgcgacct gctgattgcg ctggaaagtc gtctgcatca
    10381 aaagcataaa ctggatgctg ctgcacgcct tcagtatgag caagctcgcg ataaagtcat
    10441 taagaaaatg gcagaaaaca tcccagaaat tctggttgat gagcttaaaa atgccgatat
    10501 taatcgtcgg gttaacacgc tggaacttac cgataatcaa ggcgaaaatc ttaccttcgt
    10561 attaacgcta catgacggta gcaaatgtga gttagtcgtc aatgagttgc agattgaaat
    10621 gctagcgcga gcaatcattc atgcaattaa taatgcggaa atgcgtgaac tggcattacg
    10681 tattacctca ctgttagatt tcctgccgtt atatgacgtt gactgccagg aaaatggcaa
    10741 tctggaatat gacacctatt cccaaccgga atggaaacat aacttgttcg accattatct
    10801 ggcggtgctt taccgcttta aagatgaaag tggcaaagaa caatttagcg gcgcagtggt
    10861 gaaaacgcgt gaagcaacgc cgggtaaaga agtagaggcg atcactcgcc gaatgcttga
    10921 tttcagccca cgactgaaaa aactcgcggg tgtgccatgt caggtctatg ttcgcaccgt
    10981 agcggcgaat aacgcccaac cgctaaccca ggatcaatgc ttacgcgcgc tgcatcactt
    11041 acgtgttcaa tccaccagca aaactgcacc acaggcgaaa taatctcatc tttgctaatg
    11101 cgaggctgga gccatccagc ctgaaatgac tgataacgtg atcgttggtc atgccggatg
    11161 cggcgtaaac gccttatccg gcctacaa
//
LOCUS       DAZPEB010000525        10871 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.guided.139, whole genome shotgun sequence.
ACCESSION   DAZPEB010000525 DAZPEB010000000
VERSION     DAZPEB010000525.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10871
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.guided.139"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            1..650
                     /locus_tag="ACHURJ_004541"
                     /pseudo
     CDS             1..650
                     /locus_tag="ACHURJ_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            647..1261
                     /gene="prfH"
                     /locus_tag="ACHURJ_004542"
     CDS             647..1261
                     /gene="prfH"
                     /locus_tag="ACHURJ_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFR1283161.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1318..2775)
                     /gene="pepD"
                     /locus_tag="ACHURJ_004543"
     CDS             complement(1318..2775)
                     /gene="pepD"
                     /locus_tag="ACHURJ_004543"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFR1283162.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3036..3494
                     /gene="gpt"
                     /locus_tag="ACHURJ_004544"
     CDS             3036..3494
                     /gene="gpt"
                     /locus_tag="ACHURJ_004544"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFR1283163.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3586..4830
                     /gene="frsA"
                     /locus_tag="ACHURJ_004545"
     CDS             3586..4830
                     /gene="frsA"
                     /locus_tag="ACHURJ_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFR1283164.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIVMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4888..5289
                     /gene="crl"
                     /locus_tag="ACHURJ_004546"
     CDS             4888..5289
                     /gene="crl"
                     /locus_tag="ACHURJ_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFR1283165.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5328..6383)
                     /gene="phoE"
                     /locus_tag="ACHURJ_004547"
     CDS             complement(5328..6383)
                     /gene="phoE"
                     /locus_tag="ACHURJ_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFR1283166.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGGEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAIGMTYQF"
     gene            6671..7774
                     /gene="proB"
                     /locus_tag="ACHURJ_004548"
     CDS             6671..7774
                     /gene="proB"
                     /locus_tag="ACHURJ_004548"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFR1283167.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            7786..9039
                     /gene="proA"
                     /locus_tag="ACHURJ_004549"
     CDS             7786..9039
                     /gene="proA"
                     /locus_tag="ACHURJ_004549"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFR1283168.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTCDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            9154..9229
                     /locus_tag="ACHURJ_004550"
     tRNA            9154..9229
                     /locus_tag="ACHURJ_004550"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9187..9189,aa:Thr,seq:cgt)
     gene            complement(9244..10362)
                     /locus_tag="ACHURJ_004551"
     CDS             complement(9244..10362)
                     /locus_tag="ACHURJ_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1283169.1"
                     /translation="MPNGKRFKQSLGTKDKRQATELHDKLKAEAWRVSKLGEIPDITF
                     EEACVRWLEEKAHKKSLDDDKSRIGFWLQHFAGMQLRDITESKIYSAMQKMTNRRHEE
                     NWKLRAEACRKKGKPVPEYTPKPASVATKATHLSFIKALLRAAEREWKMLDKAPIIKV
                     PQPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSNIINLEWQQIDMQRRV
                     AWINPEESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKESCTKPDGTKAPTVRKM
                     RYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVLQEMGGWESIEMVRRY
                     AHLAPNHLTEHARQIDSILNPSVPNLSQSRNKERTNDV"
     gene            complement(10284..10718)
                     /locus_tag="ACHURJ_004552"
     CDS             complement(10284..10718)
                     /locus_tag="ACHURJ_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1283170.1"
                     /translation="MGYDSRLDRLATTSWHSFFNNITARGEIMEPYSLTLDEACHFLK
                     ISRPTAINWIRTGRLQATRKDPTKNKSPYLTTRQACIAALQSPLHTVQVSAGDGITEE
                     RKCHSSAEVKYGTPVSHCRTVKDLNSLLEQRTKGRRQNSMTS"
     gene            complement(10634..>10871)
                     /locus_tag="ACHURJ_004553"
     CDS             complement(10634..>10871)
                     /locus_tag="ACHURJ_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1283171.1"
                     /translation="FRKECPLNSGYLISLEVEPDERESYELFMEKHFGGLVDSRRAKN
                     GDNEYMAWDMTLGWIVWQQRAGIHFSTISQQEVK"
BASE COUNT         2590 a         2676 c         2805 g         2800 t
ORIGIN      
        1 caccccatta taaggggtac gaataaagta acggggcagt ctattttaca gcggcatatt
       61 gcctcgttct cgcatcatgg tttgcctcaa ggcagtgacg acgcgctacg gtatattgcg
      121 gaacatgacg atgcgctggc gtttgcgtgt attaatcgcc agatgatcgc tttgcgcata
      181 atgcaacaag ttaaggccac gggaagtccg gttctggatg tggcgcataa ctttgttagc
      241 gcgtgtcaaa tcggcgtcaa cagggctggt tgcatcgtaa aggtgccacg ccggatgaca
      301 acggtctggt gattattccc ggttcacgtg gtgattactc ctggattgta cagcctgttg
      361 cgaacgaaaa aacgctccac tcgctggcgc atggggcagg gcgtaaatgg gggcgcaccg
      421 agtgtaaatg gcgtctggca gcgaaataca cggtgacgca actctcgcgg actgaacttg
      481 gcagtcggat aatttgtcgc gataaacaac tcatctttga agaagcgcca caagcctata
      541 aatcggctga aagcgtggtg caatgtctgg tgcaggccgg tttaattatt cctgtcgcgc
      601 ggctgcgtcc ggtgctaacg ctcaaaaaca gtggagggaa aaaaggatga tcttgctaca
      661 actctcctca gcgcaggggc cggaagaatg ttgtctcgca gtgaaaaagg cgctggacag
      721 gctaattaaa gaagctgccc gacaagacgt cgcggtaacg gtgttggaaa cagaaacggg
      781 ccgctactct gacacgctgc gttcagctct ggtttctctg gatggcgata acgcatgggc
      841 attaagcgaa agctggtgtg gcactattca gtggatttgt ccgagtccgt atcggcctca
      901 tcatgggcgc aaaaactggt ttctgggcat tgggcgtttt accgctgatg agcaggagca
      961 atccgatgcg atccggtatg agacgctgcg ttcgtcgggg ccgggcggtc aacatgtcaa
     1021 taaaaccgac tcggcggtac gcgccacgca tttggcatcc ggtattagcg tgaaggttca
     1081 gtcagagcgt agtcagcatg ctaacaagcg gctggcacga ttgctgattg cctggaagct
     1141 ggagcagcag caacaggaaa atagcgcggt gctgaaatcg cagcggcgaa tgttccatca
     1201 ccagattgaa cgtggcaacc cgagacggac atttacaggg atggctttta tcgaaggata
     1261 atgaaggatg aaactgccgg aaggcgatta aacgccatcc ggcagcaaat caaataatta
     1321 cttcgccgga atttctttca gcagttcagt cagcagtgtc cagtaatgac ctacgctttc
     1381 gatgtgaact tgctcatccg gagagtgtgg accggtgata gttggcccga tagaaaccat
     1441 gtccatttcc ggatacggtt ttttgaacag accacattcc aggcccgcgt ggataatctg
     1501 gatgttcggc gtcttgttga acaggcgctg ataggtttca cgtaccagat gcatcaccgg
     1561 agaattagcg tccggctgcc agccaggata tgcgcctttc gcttcggttt tcgcgccagc
     1621 cagtttaccc agcgaatcca gcatgctcac cacgtagtct ttaccgctgt cgatcagtga
     1681 acggatcagg cagtgaattt ctacgttatt gtcagtcatg gtcaccacac cgacgttcag
     1741 ggaggtttca accacacctt tggctacatc ggagttgcgg atcacaccgt tcggggtggc
     1801 gttcagcaga cgaataaagg tatcgcgaga tttcgcaatc agggcagctt tatcgttcgc
     1861 tacagagtcc agcaacaagg ccagattttt ctctttctct gccagctcgt ttttcaggat
     1921 ctcctgatag gtattcacca gagatttcag tgcgtcgact ttatcagctg cgacagcaat
     1981 ggtcgcaaag gcttcacgcg ggatggcgtt acgcagtgtg ccgccgttga aatcgataag
     2041 gcgcaggtcc agttcttctg catgacccgc caggaagcgc accagcagtt tgttggcatt
     2101 acccaggcca acgtggattt caccgccgga gtgaccgcct ttcagacctt ttaaggttaa
     2161 cttgaaggtt tcaaaaccag ctggaaccgc ttcacgatct aaatgcaggt tggaggtgaa
     2221 gtcgataccc cccgcacaac ccatgtagat ttcaccttct tcttcggagt cggtgttaat
     2281 cagaatatca gcctgcaacc agttgctctg taagccaaaa gcaccgtcca taccggcttc
     2341 ttcggtcatg gtcagcagca cttccagcgg accgtgaacc acgttttcgt cagccagaac
     2401 cgccagtgca gaggccatac caatgccgtt atccgcaccc agcgtggtgc cgcgcgcttt
     2461 aacccattcg ccatcaatat aaggctggat aggatctttc gtgaagtcat gcacggtgtc
     2521 gttatttttc tgcggcacca tatcgaggtg ggcctgtaag acgaccggtt tacgattttc
     2581 catacctgcg gtagcaggtt tacgaatcag gatattacct acctgatcgc gttcgacatg
     2641 gaaacctttc tcttttgccc aaccaacaat gtattcagcg agttgctctt catgatagga
     2701 cgggtgcgga atagaacaga ttttggcaaa aatatcccac agcggctgtg gagataattg
     2761 agacagttca gacacgttaa gtctccttgt cgatcacccg caaaacagta ttgcaggtca
     2821 cagggttagc agaaaatgtt gtcaacacaa gacaggcttg cgagatatgt ttgagaatac
     2881 cactttatcc cgcgtcaggg agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac
     2941 tggtttttag tgcgccagat ctctataatc tcgcgcaacc tattttcccc tcgaacactt
     3001 tttaagccgt agataaacag gctgggacac ttcacatgag cgaaaaatac atcgtcacct
     3061 gggacatgtt gcagatccat gcacgtaaac tcgcaagccg actgatgcct tctgaacaat
     3121 ggaaaggcat tattgccgta agccgtggcg gtctggtacc gggtgcgtta ctggcgcgtg
     3181 aactgggtat tcgtcatgtc gataccgttt gtatttccag ctacgatcac gacaaccagc
     3241 gagagcttaa agtgctgaaa cgcgcagaag gcgatggcga aggcttcata gttattgatg
     3301 acctggtgga taccggtggt actgctgttg cgattcgtga aatgtatcca aaagcgcact
     3361 ttgtcaccat cttcgcaaaa ccggctggtc gtccgctggt tgatgactat gttgttgata
     3421 ttccgcaaga tacctggatc gaacagccgt gggatatggg cgtcgtattc gtcccgccaa
     3481 tctccggtcg ctaatctttt caacgcctgg cactgccggg cgttgttctt tttaacttca
     3541 ggcaggttac aatagtttcc agtaagtatt ctggaggctg catccatgac acaggcaaac
     3601 ctgagcgaaa ccctgttcaa accccgcttt aaacatcctg aaacctcgac gctagtccgc
     3661 cgctttaatc acggcgcaca accgcctgtg cagtcggctc ttgatggtaa aaccatccct
     3721 cactggtatc gcatgattaa ccgtctgatg tggatctggc gcggtattga cccacgtgaa
     3781 atcctcgacg tccaggcacg tattgtgatg agcgatgctg aacgtaccga cgatgattta
     3841 tacgatactg tgattggcta ccgtggtggc aactggattt atgagtgggc cacccaggcg
     3901 atggtgtggc aacaaaaagc ctgtgcggaa gaagatccgc aactcagtgg tcgacactgg
     3961 ctgcatgcgg ctacgttgta caacattgcc gcctatcctc atctgaaagg agatgacctg
     4021 gccgagcaag cgcaggcttt atcaaaccgc gcctatgaag aggccgccca gcgtctgccg
     4081 ggcacgatgc ggcagatgga gtttaccgta cccggcggtg cgcccatcac cggctttttg
     4141 catatgccga aaggcgatgg cccgttcccg acagtattaa tgtgtggtgg tctggatgcg
     4201 atgcagacgg actattacag cctgtatgaa cgttattttg cgccgcgcgg cattgtgatg
     4261 ctgactattg atatgccgtc ggtgggcttt tcttcaaaat ggaagctcac ccaggactcc
     4321 agcctgttgc atcagcacgt cttaaaggcg ctgcctaacg taccgtgggt ggatcacact
     4381 cgcgtcgcgg cctttggttt ccgtttcggc gctaacgttg ccgtgcgtct ggcatatctt
     4441 gaatcgccgc gtctgaaagc ggttgcctgt cttggtccgg tagttcatac cctgttgagt
     4501 gattttaagt gccagcaaca ggtgccggaa atgtatcttg acgttctggc gagtcgtttg
     4561 gggatgcatg atgcttccga cgaagcgtta cgcgtggagc tgaatcgcta ttcattaaaa
     4621 gtgcaaggat tgctgggacg tcgctgccca acgccaatgt tatcaggcta ctggaagaac
     4681 gatccgttca gcccggaaga ggactcacgc ttaatcacct catcatctgc tgacggtaaa
     4741 ttattagaga tcccatttaa cccggtgtat cggaattttg acaaaggtct tcaggaaatc
     4801 accggctgga tcgaaaaacg cttgtgttaa aaatttgcta aattttgcca atttggtaaa
     4861 acagttgcat cacaacagga gatagcaatg acgttaccga gtggacaccc gaagagcaga
     4921 ttgatcaaaa aatttaccgc actaggcccg tatattcgtg aaggtaagtg cgaagataat
     4981 cgattctttt tcgattgtct ggctgtatgc gtcaacgtga aaccggcacc ggaagtgcgt
     5041 gaattctggg gctggtggat ggagctggaa gcgcaggaat cccgttttac ctacagctac
     5101 cagtttggtc tgttcgataa agcaggcgac tggaagagtg ttccggtaaa agatactgaa
     5161 gtggttgaac gactggagca caccctgcgt gagtttcacg agaagctgcg tgaactgctg
     5221 acgacgctga atctgaagct ggaaccggcg gatgattttc gtgacgagcc ggtgaagtta
     5281 acggcgtgag tgaaatgtgc cggatgcatc acatccggca atatctatta aaactgatac
     5341 gtcatcccaa tcgcgacaat atcatcatta ttaatattca atttgttatc gctatccagt
     5401 tggttgattt tataatcaac aaatgctgac atatttttgt tgaaataata cgtagcaccg
     5461 acgtcgatat aattaaccag atcttcacca ccgatgccct caatatcttt ccctttcgat
     5521 aagacataac ccagcgatgg acgcagacca aagtcaaact ggtattgagc gaccgcttca
     5581 aagttctgtg tcttattggc aaagccgcca gttattggcg tcattttgcg agtttcagaa
     5641 tagaaagttg ccagataaat attattggca tcgtatttca gacctgttgc ccatgcttct
     5701 gcacgcttgc ctgtgccacg gctttgcagg ttctgctcgt tggtgcgatc tgagttggta
     5761 taggccccac taatggcgaa atcgctgccg ccaaagtcat atgtcaatga cgtgccgaag
     5821 ccatcgccgt tttgcttttt aacgtcgcgg ttttcgtttt tcccttgata ttgcagggtt
     5881 aagttcaggc catcgataac gccgaagaag tcggtgttcc gatacgtcgc cagaccgctg
     5941 gcgcgtttgg tcataaagtt gtcggtctgc gccgaggagt cgccaccaaa ttccggaaac
     6001 atatcggtcc aggcttccac gtcatacaac gcgcccaggt tacgaccata atcgaaagaa
     6061 cccaaatctt tatatttcaa cccggcaaaa gcgagacgcg ttttttgctg tgcagtatca
     6121 ctctctgctt tattaccggc aaactctgct tcccaacgac cataaccagt cagttgatcg
     6181 ttaatttgtg tttcgccttt aaaaccaaaa cggatataac tctggtcgcc atctttactg
     6241 tcgttatcac tcatataatg catggcttta actttgccat agacatccag tttattaccg
     6301 tctttgttat atatttctgc ggcctgtacg gatgcagatg ccacaatgcc catcaccact
     6361 aatgccagag tgctcttttt cattttcatt cctgatttta attaacgcgc gaatattcag
     6421 cgggagagcc ccgttgaaaa caggaaagtt tttaacctga gattgttaaa gatatattac
     6481 agattaataa tattcttaaa atgtggtaat ttattaaatc tgtaataaaa gcgtaaacaa
     6541 ctgccgctag gcttgctgat cccgcgcaac aaaacgccat gctttgctcg cagatggttg
     6601 gcaaccgacg acagtcctgc taaaacgttc gtttgatatc atttttccta aaattgaatg
     6661 gcagagaatc atgagtgaca gccagacgct ggtggtaaaa ctcggcacca gtgtgctaac
     6721 aggcggatcg cgccgtctga accgtgccca tatcgttgaa cttgttcgcc agtgcgcgca
     6781 gttacatgcc gccgggcatc ggattgttat tgtgacgtcg ggcgcgatcg ccgccggacg
     6841 tgagcacttg ggttacccgg aactgccagc gactatcgcc tcgaaacaac tgctggcagc
     6901 ggtagggcag agtcgactga ttcaactgtg ggaacagctg ttttcgattt atggcattca
     6961 cgtcgggcaa atgctgctga cccgtgctga tatggaagac cgtgaacgct tcctgaacgc
     7021 ccgcgacacc ctgcgtgcgt tgctcgataa caatatcgtt ccggtaatca atgagaacga
     7081 tgctgtcgct acggcagaga ttaaagtcgg cgataacgat aacctttctg cgctggcggc
     7141 gattctggcg ggtgccgata aactgttgct gctgaccgat caaaaaggtt tgtataccgc
     7201 tgacccgcgc agcaatccgc aggcagaact gattaaagat gtttacggca ttgatgacgc
     7261 actgcgcgcg attgccggtg acagcgtttc aggcctcgga actggcggca tgagtaccaa
     7321 attgcaggcc gctgacgtgg cttgccgtgc gggtatcgac accattattg ccgcgggcag
     7381 caagccgggc gttattggtg atgtgatgga aggcatttcc gtcggtacgc tgttccatgc
     7441 ccaggcgact ccgcttgaaa accgtaaacg ctggattttc ggtgcgccgc cggcgggtga
     7501 aatcacggta gatgaagggg caactgccgc cattctggaa cgcggcagct ccctgttgcc
     7561 gaaaggcatt aaaagcgtga ctggcaattt ctcgcgtggt gaagtcatcc gcatttgcaa
     7621 cctcgaaggt cgcgatatcg cccacggtgt cagtcgttac aacagcgatg cattacgccg
     7681 tattgccgga caccactcgc aagaaattga tgcaatactg ggatatgaat acggcccggt
     7741 tgccgttcac cgtgatgaca tgattacccg ttaaggagct ggccgatgct ggaacaaatg
     7801 ggcattgccg cgaagcaagc ctcgtataaa ttagcgcaac tctccagccg cgaaaaaaat
     7861 cgcgtgctgg aaaaaatcgc cgatgaactg gaagcacaaa gcgaaatcat tctcaacgct
     7921 aacgcccagg atgttgctga cgcgcgagcc aatggcctta gcgaagcgat gcttgaccgt
     7981 ctggcactga cgcccgcacg gctgaaaggc attgccgacg atgtacgtca ggtgtgcaac
     8041 ctcgccgatc cggtggggca ggtaatcgat ggcggcgtac tggacagcgg cctgcgtctt
     8101 gagcgtcgtc gcgtaccgct gggagttatt ggcgtgattt atgaagcgcg cccgaacgtg
     8161 acggttgatg tcgcttcgct gtgcctgaaa accggtaatg cggtgatcct gcgcggtggc
     8221 aaagaaacgt gtcgcactaa cgctgcaacg gtggcggtga ttcaggacgc cctgaaatcc
     8281 tgcggcttac cggcgggtgc cgtgcaggcg attgataatc ctgaccgtgc gctggtcagt
     8341 gaaatgctgc gtatggataa atacatcgac atgctgatcc cgcgtggtgg cgctggtttg
     8401 cataaactgt gccgtgaaca gtcgacaatc ccggtgatca caggtggtat aggcgtatgc
     8461 catatttacg ttgatgaaag tgtagagatc gctgaagcat taaaagtgat cgtcaacgcg
     8521 aaaactcagc gtccgagcac atgtaatacg gttgaaacgt tgctggtgaa taaaaacatc
     8581 gccgatagct tcctgcccgc attaagcaaa caaatggcgg aaagcggcgt gacattacac
     8641 gcagatgcag ctgcactggc gcagttgcag gcaggccctg cgaaggtggt tgctgttaaa
     8701 gccgaagagt atgacgatga gtttctgtca ttagatttga acgtcaaaat cgtcagcgat
     8761 cttgacgatg ccatcgccca tattcgtgaa cacggcacac aacactccga tgcgatcctg
     8821 acctgcgata tgcgcaacgc ccagcgtttt gttaacgaag tggattcgtc cgctgtttac
     8881 gttaacgcct ctacgcgttt taccgacggc ggccagtttg gtctgggtgc ggaagtggcg
     8941 gtaagcacac aaaaactcca cgcgcgtggc ccaatggggc tggaagcact gaccacttac
     9001 aagtggatcg gcattggtga ttacaccatt cgtgcgtaaa taaaaccggg tgatgcaaaa
     9061 gtagccattt gattcacaag gccattgacg catcgcccgg ttagttttaa ccttgtccac
     9121 cgtgattcac gttcgtgaac atgtcctttc agggccgata tagctcagtt ggtagagcag
     9181 cgcattcgta atgcgaaggt cgtaggttcg actcctatta tcggcaccat ttaaatcaat
     9241 aagttacaca tcattagtac gttccttatt tcttgactgg gacaaatttg ggaccgatgg
     9301 gttcagaatc gagtctattt gccgtgcgtg ttcggtaagg tgattaggtg cgaggtgagc
     9361 atatcgacga accatttcga tagactccca gcctcccatt tcctgtaaca ctgataacgg
     9421 gactccggct tgaaccagcc aacttgccca ggtgtgtctc aagtcgtgaa atctgaaatc
     9481 atcaatacct gctcgtctca gcgccgcttt ccaggctgtg tttgcgtcat accgcatctt
     9541 cctgactgtt ggcgctttcg ttccgtctgg tttggtacag ctttccttat acacaaatac
     9601 ccaacggtga tgattcccga tttgtttttt caatacgcga catgcagtat cattcagcgc
     9661 aacgccaatt gcgcggtttg atttactctc ttccgggttt atccatgcca cccggcgctg
     9721 catgtctatt tgttgccatt caaggttgat gatgttcgag cgtcttaagc ctgttgcaag
     9781 tgcaaattca acaacagact ttaatggctc cggacattca tcaatcagcc tttgtgcttc
     9841 atgaggctcc agccagcgga tccgtttatt ctttggttga ggcactttaa taattggtgc
     9901 cttatccagc attttccatt cacgctctgc ggctcttagc agggccttta taaatgaaag
     9961 atgcgtagcc ttcgttgcaa cggacgctgg ttttggcgtg tattctggaa caggtttccc
    10021 tttttttctg catgcttctg ccctgagttt ccagttttcc tcatgacgcc ggttcgtcat
    10081 tttctgcatt gctgaataaa tttttgattc agtaatgtct cttagttgca ttcctgcgaa
    10141 atgttgaagc cagaatccga tccggctttt gtcatcgtcc agtgattttt tatgtgcttt
    10201 ctcttcaagc cacctgacac acgcttcctc gaacgttata tcaggaattt caccaagttt
    10261 gctgacccgc catgcttcag cctttagctt gtcatggagt tctgtcgcct gccttttgtc
    10321 ctttgttcca agagactgtt taaatctttt accgttcggc aatgtgaaac tggcgtacca
    10381 tatttcacct ctgcggaaga gtgacatttt ctttcctctg ttatgccatc acccgcgctc
    10441 acctggacag tatgcagcgg agactgaagc gccgcaatgc aggcttgtcg tgttgtgagg
    10501 taaggagatt tattcttagt gggatctttg cgtgttgcct gcagacgccc tgtgcgtatc
    10561 cagttaatgg cagtcggtct ggatatcttg agaaaatgac aggcctcatc tagcgtgagg
    10621 ctgtatggtt ccattatttc acctcttgct gtgatattgt tgaaaaatga atgccagctc
    10681 gttgttgcca gacgatccaa ccgagagtca tatcccatgc catgtattcg ttatcgccgt
    10741 tttttgctct ccgactatct actaagccac cgaaatgttt ttccataaat aattcatagg
    10801 attcgcgttc atctggctct acttccagag atatgaggta gcctgagttt aacggacact
    10861 ccttcctgaa a
//
LOCUS       DAZPEB010000526         7787 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.guided.180, whole genome shotgun sequence.
ACCESSION   DAZPEB010000526 DAZPEB010000000
VERSION     DAZPEB010000526.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7787
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.guided.180"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            23..1285
                     /locus_tag="ACHURJ_004554"
     CDS             23..1285
                     /locus_tag="ACHURJ_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031587700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISEcp1 family transposase"
                     /protein_id="HFR1283172.1"
                     /translation="MINKIDFKAKNLTSNAGLFLLLENAKSNGIFDFIENDLVFDNDS
                     TNKIKMNHIKTMLCGHFIGIDKLERLKLLQNDPLVNEFDISVKEPETVSRFLGNFNFK
                     TTQMFRDINFKVFKKLLTKSKLTSITIDIDSSVINVEGHQEGASKGYNPKKLGNRCYN
                     IQFAFCDELKAYVTGFVRSGNTYTANGAAEMIKEIVANIKSDDLEILFRMDSGYFDEK
                     IIETIESLGCKYLIKAKSYSTLTSQATNSSIVFVKGEEGRETTELYTKLVKWEKDRRF
                     VVSRVLKPEKERAQLSLLEGSEYDYFFFVTNTTLLSEKVVIYYEKRGNAENYIKEAKY
                     DMAVGHLLLKSFWANEAVFQMMMLSYNLFLLFKFDSLDSSEYRQQIKTFRLKYVFLAA
                     KIIKTARYVIMKLSENYPYKGVYEKCLV"
     gene            1541..2416
                     /locus_tag="ACHURJ_004555"
     CDS             1541..2416
                     /locus_tag="ACHURJ_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011091028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extended-spectrum class A beta-lactamase
                     CTX-M-15"
                     /protein_id="HFR1283173.1"
                     /translation="MVKKSLRQFTLMATATVTLLLGSVPLYAQTADVQQKLAELERQS
                     GGRLGVALINTADNSQILYRADERFAMCSTSKVMAAAAVLKKSESEPNLLNQRVEIKK
                     SDLVNYNPIAEKHVNGTMSLAELSAAALQYSDNVAMNKLIAHVGGPASVTAFARQLGD
                     ETFRLDRTEPTLNTAIPGDPRDTTSPRAMAQTLRNLTLGKALGDSQRAQLVTWMKGNT
                     TGAASIQAGLPASWVVGDKTGSGGYGTTNDIAVIWPKDRAPLILVTYFTQPQPKAESR
                     RDVLASAAKIVTDGL"
     gene            complement(2463..2807)
                     /locus_tag="ACHURJ_004556"
                     /pseudo
     CDS             complement(2463..2807)
                     /locus_tag="ACHURJ_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016943399.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
     gene            2821..5571
                     /locus_tag="ACHURJ_004557"
                     /pseudo
     CDS             2821..5571
                     /locus_tag="ACHURJ_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611787.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
     gene            complement(5647..6662)
                     /locus_tag="ACHURJ_004558"
                     /pseudo
     CDS             complement(5647..6662)
                     /locus_tag="ACHURJ_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            7057..7713
                     /locus_tag="ACHURJ_004559"
     CDS             7057..7713
                     /locus_tag="ACHURJ_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001516695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrS1"
                     /protein_id="HFR1283174.1"
                     /translation="METYNHTYRHHNFSHKDLSDLTFTACTFIRSDFRRANLRDTTFV
                     NCKFIEQGDIEGCHFDVADLRDASFQQCQLAMANFSNANCYGIEFRACDLKGANFSRT
                     NFAHQVSNRMYFCSAFISGCNLSYANMERVCLEKCELFENRWIGTNLAGASLKESDLS
                     RGVFSEDVWGQFSLQGANLCHAELDGLDPRKVDTSGIKIAAWQQELILEALGIVVYPD
                     "
BASE COUNT         2069 a         1852 c         1897 g         1969 t
ORIGIN      
        1 gtagtaaaaa ggggttctaa ttatgattaa taaaattgat ttcaaagcta agaatctaac
       61 atcaaatgca ggtctttttc tgctccttga gaatgcaaaa agcaatggga tttttgattt
      121 tattgaaaat gacctcgtat ttgataatga ctcaacaaat aaaatcaaga tgaatcatat
      181 aaagaccatg ctctgcggtc acttcattgg cattgataag ttagaacgtc taaagctact
      241 tcaaaatgat cccctcgtca acgagtttga tatttccgta aaagaacctg aaacagtgtc
      301 acggtttcta ggaaacttca acttcaagac aacccaaatg tttagagaca ttaattttaa
      361 agtctttaaa aaactgctca ctaaaagtaa attgacatcc attacgattg atattgatag
      421 tagtgtaatt aacgtagaag gtcatcaaga aggtgcgtca aaaggatata atcctaagaa
      481 actgggaaac cgatgctaca atatccaatt tgcattttgc gacgaattaa aagcatatgt
      541 taccggattt gtaagaagtg gcaatactta cactgcaaac ggtgctgcgg aaatgatcaa
      601 agaaattgtt gctaacatca aatcagacga tttagaaatt ttatttcgaa tggatagtgg
      661 ctactttgat gaaaaaatta tcgaaacgat agaatctctt ggatgcaaat atttaattaa
      721 agccaaaagt tattctacac tcacctcaca agcaacgaat tcatcaattg tattcgttaa
      781 aggagaagaa ggtagagaaa ctacagaact gtatacaaaa ttagttaaat gggaaaaaga
      841 cagaagattt gtcgtatctc gcgtactgaa accagaaaaa gaaagagcac aattatcact
      901 tttagaaggt tccgaatacg actacttttt ctttgtaaca aatactacct tgctttctga
      961 aaaagtagtt atatactatg aaaagcgtgg taatgctgaa aactatatca aagaagccaa
     1021 atacgacatg gcggtgggtc atctcttgct aaagtcattt tgggcgaatg aagccgtgtt
     1081 tcaaatgatg atgctttcat ataacctatt tttgttgttc aagtttgatt ccttggactc
     1141 ttcagaatac agacagcaaa taaagacctt tcgtttgaag tatgtatttc ttgcagcaaa
     1201 aataatcaaa accgcaagat atgtaatcat gaagttgtcg gaaaactatc cgtacaaggg
     1261 agtgtatgaa aaatgtctgg tataataaga atatcatcaa taaaattgag tgttgctctg
     1321 tggataactt gcagagttta ttaagtatca ttgcagcaaa gatgaaatca atgatttatc
     1381 aaaaatgatt gaaaggtggt tgtaaataat gttacaatgt gtgagaagca gtctaaattc
     1441 ttcgtgaaat agtgattttt gaagctaata aaaaacacac gtggaattta gggactattc
     1501 atgttgttgt tatttcgtat cttccagaat aaggaatccc atggttaaaa aatcactgcg
     1561 ccagttcacg ctgatggcga cggcaaccgt cacgctgttg ttaggaagtg tgccgctgta
     1621 tgcgcaaacg gcggacgtac agcaaaaact tgccgaatta gagcggcagt cgggaggcag
     1681 actgggtgtg gcattgatta acacagcaga taattcgcaa atactttatc gtgctgatga
     1741 gcgctttgcg atgtgcagca ccagtaaagt gatggccgcg gccgcggtgc tgaagaaaag
     1801 tgaaagcgaa ccgaatctgt taaatcagcg agttgagatc aaaaaatctg accttgttaa
     1861 ctataatccg attgcggaaa agcacgtcaa tgggacgatg tcactggctg agcttagcgc
     1921 ggccgcgcta cagtacagcg ataacgtggc gatgaataag ctgattgctc acgttggcgg
     1981 cccggctagc gtcaccgcgt tcgcccgaca gctgggagac gaaacgttcc gtctcgaccg
     2041 taccgagccg acgttaaaca ccgccattcc gggcgatccg cgtgatacca cttcacctcg
     2101 ggcaatggcg caaactctgc ggaatctgac gctgggtaaa gcattgggcg acagccaacg
     2161 ggcgcagctg gtgacatgga tgaaaggcaa taccaccggt gcagcgagca ttcaggctgg
     2221 actgcctgct tcctgggttg tgggggataa aaccggcagc ggtggctatg gcaccaccaa
     2281 cgatatcgcg gtgatctggc caaaagatcg tgcgccgctg attctggtca cttacttcac
     2341 ccagcctcaa cctaaggcag aaagccgtcg cgatgtatta gcgtcggcgg ctaaaatcgt
     2401 caccgacggt ttgtaatagc ggaaacggaa tggggaaact cattccgttt ttgtttatcg
     2461 ccttagacgg caaaagtgct gtcgcccacc tgcgcttgcg cataccaggc cataagctcc
     2521 gtggttcctg gttctccttc cgctggagcc cagtgcgcat agtcatcggc agccacgggt
     2581 tgatagccac cgtgttttac ttcaaaaatt atgccaccgg tatccagcga cagcacggca
     2641 tgccaggttc ctgcggccat ctccagcacc gtacaggttt cccccaatat cgcccgatgg
     2701 gtgacggtac cccgatcgtc aaaattcagc accacgaaac gaccccttaa tggcaacagt
     2761 agctcgaagg tgtgagggtg tcggtgcggg cgcacgtagg tcccaggtca tattccttcc
     2821 ggcgtccggc attttaccgc cagacagctc gggattcgtg atatcaccgt tcttgcagaa
     2881 tacggtcaga gggaaaatac ccgccgtgag catgcagcgc tgatacgtca gcactatcag
     2941 tatcgtgaat ttgcctggcc ctggacattt cgccttaccc gtcttttata tacccggagc
     3001 tggataagca acgaacgtcc tggcctgctt ttcgatctgg cgacagggtg gcttatgcaa
     3061 catcgtatta ttctccccgg agccactacg ctgacccggt tgatttcaga ggtaagggaa
     3121 aaggcgacgt tgcgcctgtg gaacaaactg gcactgatac cgtcagccga acagcgttca
     3181 cagctggaga tgctgctggg gccaactgat tgcagccgcc tgtctttact ggaatcactg
     3241 aaaaagggcc ctgtgaccat cagtggtccg gcgtttaatg aagcaattga acgctggaaa
     3301 actctgaacg attttggcct gcatgctgaa aacctgagta cactcccggc tgtgcgcctg
     3361 aaaaatctcg cacgttatgc tggtatgact tcggtgttca atattgccag gatgtcaccg
     3421 cagaaaagga tggcggttct ggttgccttt gtccttgcat gggaaacgct ggcgctggat
     3481 gatgcattgg acgttctgga cgccatgctg gccgttatca tccgtgacgc cagaaagatt
     3541 gggcagaaaa aacggctccg ctcgctgaag gatctggata aatctgcatt ggcgctcgcc
     3601 agcgcatgtt cgtacctgct gaaagaagaa acaccggacg aatcgattcg tgctgaggtg
     3661 ttcagctaca tcccaaggca aaagctggct gaaatcatca cgcttgtccg tgaaattgcc
     3721 cggccctcag acgataattt tcatgaagaa atggtggagc agtacgggcg cgttcgtcgt
     3781 ttcctgcccc atctgctgaa taccgttaaa ttttcatccg cacctgccgg ggttaccact
     3841 ctgaatgcct gtgactacct cagccgggag ttcagctcac ggcggcagtt ttttgacgac
     3901 gcaccaacgg aaattatcag tcggtcatgg aaacggctgg tgattaacaa ggaaaaacat
     3961 atcacccgca ggggatacac gctctgcttt ctcagtaaac tgcaggatag tctgaggcgg
     4021 agggatgtct acgttaccgg cagtaaccgg tggggagatc ctcgtgcaag attactacag
     4081 ggtgctgact ggcaggcaaa ccggattaag gtttatcgtt ctttggggca cccgacagac
     4141 ccgcaggaag caataaaatc tctgggtcat cagcttgata gtcgttacag acaggttgct
     4201 gcacgtcttt gcgaaaatga ggctgtcgaa ctcgatgttt ctggcccgaa gccccggttg
     4261 acaatttctc ccctcgccag tcttgatgag ccggacagtc tgaaacgact gagcaaaatg
     4321 atcagtgatc tactccctcc ggtggattta acggagttgc tgctcgaaat taacgcccat
     4381 accggatttg ctgatgagtt tttccatgct agtgaagcca gtgccagagt tgatgatctg
     4441 cccgtcagca tcagcgccgt gctgatggct gaagcctgca atatcggtct ggaaccactg
     4501 atcagatcaa atgttcctgc actgacccga caccggctga actggacaaa agcgaactat
     4561 ctgcgggctg aaactatcac cagcgctaat gccagactgg ttgattttca ggcaacgctg
     4621 ccactggcac agatatgggg tggaggagaa gtggcatctg cagatggaat gcgctttgtt
     4681 acgccagtca gaacaatcaa tgccggaccg aaccgcaaat actttggtaa taacagaggg
     4741 atcacctggt acaactttgt gtccgatcag tattccggct ttcatggcat cgttataccg
     4801 gggacgctga gggactctat ctttgtgctg gaaggtcttc tggaacagga gaccgggctg
     4861 aatccaaccg aaattatgac cgatacagca ggtgccagcg aacttgtctt tggccttttc
     4921 tggctgctgg gataccagtt ttctccacgc ctggctgatg ccggtgcttc ggttttctgg
     4981 cgaatggacc atgatgccga ctatggcgtg ctgaatgata ttgccagagg gcaatcagat
     5041 ccccgaaaaa tagtccttca gtgggacgaa atgatccgga ccgctggctc cctgaagctg
     5101 ggcaaagtac aggtttcagt gctggtccgt tcattgctga aaagtgaacg tccttccgga
     5161 ctgactcagg caatcattga agtggggcgc atcaacaaaa cgctgtatct gcttaattat
     5221 attgatgatg aagattaccg ccggcgcatt ctgacccagc ttaatcgggg agaaagtcgc
     5281 catgccgttg ccagagccat ctgtcacggt caaaaaggtg agataagaaa acgatatacc
     5341 gacggtcagg aagatcaact gggcacactg gggctggtca ctaacgccgt cgtgttatgg
     5401 aacactattt atatgcaggc agccctggat catctccggg cgcagggtga aacactgaat
     5461 gatgaagata tcgcacgcct ctccccgctt tgccacggac atatcaatat gctcggccat
     5521 tattccttca cgctggcaga actggtgacc aaaggacatc tgagaccatt aaaagaggcg
     5581 tcagaggcag aaaacgttgc ttaacgtgag ttttcgttcc actgagcgtc agacccctaa
     5641 aatcctgatg ctgatgtagt cccgttttat cgtcatcacg aagctttctg ctatgccatt
     5701 gctttccggg cttcgcactg ctgtcgtaca aggctccagt cccagcaacc gggcgaacgc
     5761 cggcactgtt ggcaactgcc ttccgaagcg tttttccacc gcacccagca tgacatcctg
     5821 caccgtttct ttgtcatagc ctccggtgct cgctgcccag tcaatgatct ccctgtcgca
     5881 gcagtcctgc gcgaaggtca cccgcagctt ttcgccatta tcacagcgga actcaaagcc
     5941 atctgagcac cagcgccggt tactttccgc tactgctacg cgccccttat gggcccgctt
     6001 acggcaagga tcagcgggtt tacgttcaag aagcagatta tgcgttctca taatgcgata
     6061 cacccgcttt gcattaacca caggcaggcc gtcccgctcc gactcccgtc gcagcagcgc
     6121 ccacacacgg cgataaccat acgtcggcag gtcagccacc gccatgttta tccgggacaa
     6181 tacggtggta tcgtcagaac ggggctgccg tctgcgatcc tgtcagtcag atggccggtg
     6241 aacccgaatg ctcaactgcg cacgcgacac gccaagactt ctgcaaacgt cgcttattcg
     6301 tcgttcccag gcaacgaggg cgcatgcgca atccattttt ttgcccggcc gaactccacg
     6361 gcctctttga gtatctcggc ttccatcgtc tttttgccca gaaggcgctg gagttcgcga
     6421 atttgcttat tggcggcagc gagttcggag gccggcacca cttcctcgcc cgatgcaaca
     6481 gccgtcagtg aaccgtctta atattgcttg cgccatttga atatctggtt tgcattgatg
     6541 ccatgcaggc gcgcgacatg agacacggtc ataccgggct ccatagtctg ctgaataatg
     6601 gcaatttttt cctgcggtgt acgacgccga cgccgctcag gtcctgataa cacttcaacc
     6661 atcttatcgt tctgactggt attaaacata gttccaagac tacctcttat tttaagagag
     6721 tcgaagtgtc tggtgatcta tggggccagt ctagccctct tccaagtgta gggagatata
     6781 gtcaaagttt gcttctccgc cgatgagtcc gccctgcgtc ggatagtccg attttttacc
     6841 ggcgaagcac ggctctccta accctatcta aagcgaataa cttttttcaa cagggccgcg
     6901 tgccaatgca agagcgatag gcgctctacg cgccaatttg accacttaaa acaggtaaat
     6961 tgaggggttg taatgtgttg atgtaacagg cttttatttt aatgtcttgg catatgtata
     7021 atggtagtct agccctcctt tcaacaagga gtactcatgg aaacctacaa tcatacatat
     7081 cggcaccaca acttttcaca taaagactta agtgatctca ccttcaccgc ttgcacattc
     7141 attcgcagcg actttcgacg tgctaacttg cgtgatacga cattcgtcaa ctgcaagttc
     7201 attgaacagg gtgatatcga aggctgccac tttgatgtcg cagatcttcg tgatgcaagt
     7261 ttccaacaat gccaacttgc gatggcaaac ttcagtaatg ccaattgcta cggtatagag
     7321 ttccgtgcgt gtgatttaaa aggtgccaac ttttcccgaa caaactttgc ccatcaagtg
     7381 agtaatcgta tgtacttttg ctcagcattt atttctggat gtaatctttc ctatgccaat
     7441 atggagaggg tttgtttaga aaaatgtgag ttgtttgaaa atcgctggat aggaacgaac
     7501 ctagcgggtg catcactgaa agagtcagac ttaagtcgag gtgttttttc cgaagatgtc
     7561 tgggggcaat ttagcctaca gggtgccaat ttatgccacg ccgaactcga cggtttagat
     7621 ccccgcaaag tcgatacatc aggtatcaaa attgcagcct ggcagcaaga actgattctc
     7681 gaagcactgg gtattgttgt ttatcctgac taattgcttt gatgtgtgat tttaaacgct
     7741 caaatttata aaacgaataa tattgtcaac cttgcaataa tagacat
//
LOCUS       DAZPEB010000527         7085 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.guided.194, whole genome shotgun sequence.
ACCESSION   DAZPEB010000527 DAZPEB010000000
VERSION     DAZPEB010000527.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7085
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.guided.194"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(<1..142)
                     /locus_tag="ACHURJ_004560"
     CDS             complement(<1..142)
                     /locus_tag="ACHURJ_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014205641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HFR1283175.1"
                     /translation="MLTDTKLRNLKPRDKLYKVNDREGLYVGVASENGIYGHSRFCNT
                     DFD"
     gene            472..1287
                     /gene="sul2"
                     /locus_tag="ACHURJ_004561"
     CDS             472..1287
                     /gene="sul2"
                     /locus_tag="ACHURJ_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HFR1283176.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1348..2151
                     /locus_tag="ACHURJ_004562"
                     /note="aph(3'')-Ib"
     CDS             1348..2151
                     /locus_tag="ACHURJ_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HFR1283177.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            2151..2987
                     /locus_tag="ACHURJ_004563"
     CDS             2151..2987
                     /locus_tag="ACHURJ_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HFR1283178.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(3094..3498)
                     /locus_tag="ACHURJ_004564"
                     /pseudo
     CDS             complement(3094..3498)
                     /locus_tag="ACHURJ_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            3699..4244
                     /locus_tag="ACHURJ_004565"
     CDS             3699..4244
                     /locus_tag="ACHURJ_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HFR1283179.1"
                     /translation="MVWRRLRIAADTSLAALHFIFQIVQGWGDDHLHQFHIYGKDYGI
                     SYEGGIGFVDNPFRVVIDDFAFDAGDRFTYEYNFFEHWLHDIRVEAIYENSTLKAPFC
                     ISGHGMPGATAADEFDKTLAFLEAIVNADDETTVGEIRPFADDLDAVRFNRHKINRQL
                     SRLDLASPVLEPEVIWLGRRR"
     gene            4381..4953
                     /locus_tag="ACHURJ_004566"
     CDS             4381..4953
                     /locus_tag="ACHURJ_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR1283180.1"
                     /translation="MQYAYIRVSSADQNTARQEEALSKAGFQPDKICVEHASAKDTNR
                     PGLQELLGQLRPGDTLLVHSIDRLCRNMSDMCAVTTRLRDQGVTLIFLKEQLTFSAGT
                     NNPMQELQLHMMSAFSQFERALLKERQADGIAAKKARGEKTGRPGADIKKIQEIDALR
                     SRGVRLRIACDHAGLGVSTYYKLRHQMNNQ"
     gene            4990..6381
                     /locus_tag="ACHURJ_004567"
     CDS             4990..6381
                     /locus_tag="ACHURJ_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISKra4-like element ISKpn19 family transposase"
                     /protein_id="HFR1283181.1"
                     /translation="MQLTLQIVITDESGSSRTEELMTIQKSGETRNDIGLSVSESKLL
                     LNTVQQSVVQLQADEYTQHHIRCPHCLAARRIKGKQKIRYRTLFGVIPVSGLRVYRCR
                     CEESDTKTVSLLSDWAGDYSHPALKYIETRWASMISYEMTTRLLKDILPVGHSLNAST
                     VRNHLCQVAQRLDAEAEAHSGFLSGCPRDWGNLPRPGKPLVVGIDGGYVRDRDDKKRN
                     FEIIAGKSFSVGAPADTRRFGFVQKDDCHPERRLMTHLSAQGMQANQQIFFLSDGADN
                     LRDLQFGMYPESTHVLDWFHITMRLKVLMQYARGLLVSDPEAGSKVLALLESIKRYLW
                     HGNVVAALEHIDNCVMYCDDPELSYPSLKSLQKHLDEMYTYIRNNKMMIPNYGEMRRY
                     GEPVSTAFVESTINEVIARRMAKKQQMQWSRKGAHYLLQTRTAVLNNELQDKFVCWYP
                     GFQSDGKGPAMAA"
     gene            6414..6842
                     /locus_tag="ACHURJ_004568"
                     /pseudo
     CDS             6414..6842
                     /locus_tag="ACHURJ_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003155741.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT         1634 a         1861 c         1908 g         1682 t
ORIGIN      
        1 cgtcaaaatc ggtgttgcaa aaacgggagt gaccatagat tccgttttct gaggcgaccc
       61 ccacatagag accttcccgg tcattcactt tgtagagttt gtccctgggc ttgagattgc
      121 gcagcttggt atcggtcagc atgtaccttg tccttcttcg tctccgatac ccactctatt
      181 gtaaacaaga catttttatc ttttatattc aatggcttat tttcctgcta attggtaata
      241 ccatgaaaaa taccatgctc agaaaaggct taacaatatt ttgaaaaatt gcctactgag
      301 cgctgccgca cagctccata ggccgctttc ctggctttgc ttccagatgt atgctattct
      361 gctcctgcag ctaatggagc accgcaaaca ggttactcgc ctggggattc cctttcgacc
      421 cgagcatccg tatgagactc atgctcgatt attattatta tagaagcccc catgaataaa
      481 tcgctcatca ttttcggcat cgtcaacata acctcggaca gtttctccga tggaggccgg
      541 tatctggcgc cagacgcagc cattgcgcag gcgcgtaagc tgatggccga gggggcagat
      601 gtgatcgacc tcggtccggc atccagcaat cccgacgccg cgcctgtttc gtccgacaca
      661 gaaatcgcgc gtatcgcgcc ggtgctggac gcgctcaagg cagatggcat tcccgtctcg
      721 ctcgacagtt atcaacccgc gacgcaagcc tatgccttgt cgcgtggtgt ggcctatctc
      781 aatgatattc gcggttttcc agacgctgcg ttctatccgc aattggcgaa atcatctgcc
      841 aaactcgtcg ttatgcattc ggtgcaagac gggcaggcag atcggcgcga ggcacccgct
      901 ggcgacatca tggatcacat tgcggcgttc tttgacgcgc gcatcgcggc gctgacgggt
      961 gccggtatca aacgcaaccg ccttgtcctt gatcccggca tggggttttt tctgggggct
     1021 gctcccgaaa cctcgctctc ggtgctggcg cggttcgatg aattgcggct gcgcttcgat
     1081 ttgccggtgc ttctgtctgt ttcgcgcaaa tcctttctgc gcgcgctcac aggccgtggt
     1141 ccgggggatg tcggggccgc gacactcgct gcagagcttg ccgccgccgc aggtggagct
     1201 gacttcatcc gcacacacga gccgcgcccc ttgcgcgacg ggctggcggt attggcggcg
     1261 ctgaaagaaa ccgcaagaat tcgttaactg cacattcggg atatttctct atattcgcgc
     1321 ttcatcagaa aactgaagga acctccattg aatcgaacta atattttttt tggtgaatcg
     1381 cattctgact ggttgcctgt cagaggcgga gaatctggtg attttgtttt tcgacgtggt
     1441 gacgggcatg ccttcgcgaa aatcgcacct gcttcccgcc gcggtgagct cgctggagag
     1501 cgtgaccgcc tcatttggct caaaggtcga ggtgtggctt gccccgaggt catcaactgg
     1561 caggaggaac aggagggtgc atgcttggtg ataacggcaa ttccgggagt accggcggct
     1621 gatctgtctg gagcggattt gctcaaagcg tggccgtcaa tggggcagca acttggcgct
     1681 gttcacagcc tatcggttga tcaatgtccg tttgagcgca ggctgtcgcg aatgttcgga
     1741 cgcgccgttg atgtggtgtc ccgcaatgcc gtcaatcccg acttcttacc ggacgaggac
     1801 aagagtacgc cgcagctcga tcttttggct cgtgtcgaac gagagctacc ggtgcggctc
     1861 gaccaagagc gcaccgatat ggttgtttgc catggtgatc cctgcatgcc gaacttcatg
     1921 gtggacccta aaactcttca atgcacgggt ctgatcgacc ttgggcggct cggaacagca
     1981 gatcgctatg ccgatttggc actcatgatt gctaacgccg aagagaactg ggcagcgcca
     2041 gatgaagcag agcgcgcctt cgctgtccta ttcaatgtat tggggatcga agcccccgac
     2101 cgcgaacgcc ttgccttcta tctgcgattg gaccctctga cttggggttg atgttcatgc
     2161 cgcctgtttt tcctgctcat tggcacgttt cgcaacctgt tctcattgcg gacacctttt
     2221 ccagcctcgt ttggaaagtt tcattgccag acgggactcc tgcaatcgtc aagggattga
     2281 aacctataga agacattgct gatgaactgc gcggggccga ctatctggta tggcgcaatg
     2341 ggaggggagc agtccggttg ctcggtcgtg agaacaatct gatgttgctc gaatatgccg
     2401 gggagcgaat gctctctcac atcgttgccg agcacggcga ctaccaggcg accgaaattg
     2461 cagcggaact aatggcgaag ctgtatgccg catctgagga acccctgcct tctgcccttc
     2521 tcccgatccg ggatcgcttt gcagctttgt ttcagcgggc gcgcgatgat caaaacgcag
     2581 gttgtcaaac tgactacgtc cacgcggcga ttatagccga tcaaatgatg agcaatgcct
     2641 cggaactgcg tgggctacat ggcgatctgc atcatgaaaa catcatgttc tccagtcgcg
     2701 gctggctggt gatagatccc gtcggtctgg tcggtgaagt gggctttggc gccgccaata
     2761 tgttctacga tccggctgac agagacgacc tttgtctcga tcctagacgc attgcacaga
     2821 tggcggacgc attctctcgt gcgctggacg tcgatccgcg tcgcctgctc gaccaggcgt
     2881 acgcttatgg gtgcctttcc gcagcttgga acgcggatgg agaagaggag caacgcgatc
     2941 tagctatcgc ggccgcgatc aagcaggtgc gacagacgtc atactagata tcaagcgact
     3001 tctcctatcc cctgggaaca catcaatctc accggagaat atcgctggcc aaagccttag
     3061 cgtaggattc cgccccttcc cgcaaacgac cccaaacagg aaacgcagct gaaacgggaa
     3121 gctcaacacc cactgacgca tgggttgttc aggcagtact tcatcaacca gcaaggcggc
     3181 actttcggcc atccgccgcg ccccacagct cgggcagaaa ccgcgacgct tacagctgaa
     3241 agcgaccagg tgctcggcgt ggcaagactc gcagcgaacc cgtagaaagc catgctccag
     3301 ccgcccgcat tggagaaatt cttcaaattc ccgttgcaca tagcccggca attcctttcc
     3361 ctgctctgcc ataagcgcag cgaatgccgg gtaatactcg tcaacgatct gatagagaag
     3421 ggtttgctcg ggtcggtggc tctggtaacg accagtatcc cgatcccggc tggccgtcct
     3481 ggccgccaca tgaggcatgt tccgcgtcct tgcaatactg tgtttacata cagtctatcg
     3541 cttagcggaa agttctttta ggagagcatg aaaaacctcg gggcagccat cgaatgatgc
     3601 catctacagg ggttaaaata accaccatca tttcataaca atacctgctg actatgaaga
     3661 tctacgtcat caaaatagca gttcatggag tcagtccgat ggtctggcgt cggctgagaa
     3721 ttgctgccga cacgtcactg gccgcgcttc acttcatttt ccagatagtg cagggctggg
     3781 gggacgacca tctccatcaa tttcacattt atggcaaaga ttacggtatc tcctacgaag
     3841 gaggcatcgg ttttgtcgat aatccgttcc gggtcgtgat tgatgatttt gcttttgatg
     3901 ctggcgatcg ctttacctat gaatacaatt tctttgagca ctggcttcat gacattcgtg
     3961 ttgaggccat ttatgaaaac tctacgctga aagcgccgtt ttgtataagc ggccatggta
     4021 tgcccggagc cacagctgcg gatgagttcg ataaaaccct ggcgtttctt gaagctatcg
     4081 ttaatgcgga tgatgaaaca acggtcggcg agatccgccc ttttgctgac gacctggatg
     4141 ctgtcaggtt caaccgccac aaaatcaacc ggcaactgag caggctcgac cttgcatctc
     4201 cggtactgga gcctgaagtt atctggctgg gccgtcgtcg ctgactgatg tctataaagg
     4261 tatacgtttt cagcatgcct ctcagatccc cgaaaaaatg tctataaacc tccctaaatc
     4321 agtgtttatg gattattctt taaatctcta tgatgtttaa agaccttccg gaggttttgc
     4381 gtgcaatatg cctacatcag ggtaagctcg gccgatcaga atacggcccg tcaggaagag
     4441 gcattgtcaa aggcgggttt tcagcctgat aaaatttgtg ttgagcatgc cagcgcgaag
     4501 gacacaaacc gcccggggtt acaggaactc ctggggcaat tacgccctgg tgatacgctt
     4561 ctggttcatt ccatcgatcg cctttgccgg aatatgtcgg atatgtgcgc tgtcaccacc
     4621 cggcttcgtg accagggcgt tacccttatt tttctgaaag agcagctgac gttcagcgcc
     4681 ggcacaaaca atccgatgca ggaactgcag ctgcacatga tgtcggcatt cagccagttt
     4741 gaacgagcct tactgaagga aaggcaggcc gacggcatcg ccgcgaaaaa agcacggggt
     4801 gaaaaaacag ggcgccccgg cgccgatatc aaaaagatcc aggaaattga cgcactcaga
     4861 agcagagggg tcaggctcag gatcgcctgc gaccatgcgg gccttggcgt ttccacatat
     4921 tataagcttc gtcaccagat gaacaaccag tagccgggag atagtcatcc accacagcag
     4981 gaggcaatga tgcaactgac gctccagata gtcatcaccg atgaatcagg ctccagtcga
     5041 acggaggaac tgatgacaat ccagaaatca ggggagaccc ggaacgacat cggattatcg
     5101 gtgtcagaat ccaagctgtt gctgaatacg gtccagcagt cggtggtcca gctgcaggca
     5161 gacgaatata ctcaacacca tatccggtgc cctcactgcc ttgctgcgcg cagaatcaaa
     5221 ggcaaacaga aaatacggta ccggacgctg tttggcgtta ttccggtatc cgggcttcgg
     5281 gtgtaccggt gccgttgtga agagagtgat acaaagacag tcagtttgct cagcgactgg
     5341 gccggcgatt attctcaccc ggcactgaaa tatattgaaa cccgctgggc ctcgatgatc
     5401 tcctatgaaa tgacgactcg tctgctgaaa gatattctgc cggtgggcca cagcctgaat
     5461 gcctcaacgg tgaggaatca tttatgccag gtggcgcagc gtcttgacgc tgaggctgaa
     5521 gctcattcag gttttctttc cggctgcccc cgcgactggg ggaacctgcc caggccagga
     5581 aaaccgcttg tggtgggtat agatggtggt tatgttcgcg atcgggatga caaaaagcgt
     5641 aattttgaaa ttattgccgg gaagtctttt tccgttggtg cgcctgctga cacccgccgc
     5701 ttcggttttg ttcagaagga tgactgtcat ccggaacgca ggctcatgac ccacctttca
     5761 gcgcagggaa tgcaggccaa ccagcagata ttttttctgt ccgacggcgc ggataatctc
     5821 agggacctcc agttcggtat gtaccctgag tcaacgcatg tgctggactg gtttcatatc
     5881 accatgaggc tgaaggtgct catgcaatat gcccggggac tgctggtatc agatccagag
     5941 gcggggagta aagttctggc actgctggaa agcattaaac gatatctctg gcatggcaat
     6001 gttgtcgctg cgctggaaca tattgataat tgcgttatgt attgcgatga ccctgaactc
     6061 agctatccta gcctgaaatc cctgcaaaaa catctggatg aaatgtatac ctacatccgg
     6121 aataataaga tgatgatccc aaactacggc gaaatgcgtc ggtacggaga gccggtatca
     6181 accgcgtttg tggaatccac gatcaatgaa gtgatcgcca gacgaatggc caaaaagcag
     6241 cagatgcagt ggagcagaaa aggggctcat tatttgttac agacccggac cgccgttttg
     6301 aataacgaac tgcaggataa atttgtctgc tggtatccgg gttttcaaag tgacgggaag
     6361 ggaccagcga tggcagcata gctcacgctg ccccaatatt ttttatgctc tcggtcgtat
     6421 ggaagctgga ccgcctcggt cgtgatcttc gtcatctcat taatacagtg cacgacctga
     6481 ccgcgagggg gaccggtctg aaagtgctga ccggtcacgg ggcgactatc gacacaacaa
     6541 cggccgctgg caagcttgtc ttcggtatct ttgccgcact ggcggagttt gagcgcgaac
     6601 tgattgctga aagaacgact gcaggtctgg catcagcaag agcccgtggg cggaacggcg
     6661 gccgacctta taaaatgacg ccagtcaaac tgcgactggc aatggcatca atgggacaat
     6721 cagaaacaaa ggtcagtacg ctatgccagg aacttgggat aacccggcaa actttgtaca
     6781 ggcatatttc ccctgttggt caactgcggg cggacgggat caagctgctc aaccgtggat
     6841 aatttgaacg gacggaaaaa tctggggttc cggcgtagta cccctatcac ctccacattt
     6901 ttgcaacagt gccttataaa tggcagcctt atgtttactt gccaagatgg gctaccatat
     6961 gatgttttat actgagcaat attaatatta tctttaatgg aaaaattaag cccactaagt
     7021 gacccactgc tatatggctc tattgtgaag acgcctgagg gtaaaaattt atacatggtt
     7081 gtccc
//
LOCUS       DAZPEB010000528         3332 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella boydii strain M2307618-1R
            SAMN43277784-rid23485933.guided.282, whole genome shotgun sequence.
ACCESSION   DAZPEB010000528 DAZPEB010000000
VERSION     DAZPEB010000528.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277784
            Sequence Read Archive: SRR30464065
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396416.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:19:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,572
            CDSs (total)                      :: 4,490
            Genes (coding)                    :: 4,076
            CDSs (with protein)               :: 4,076
            Genes (RNA)                       :: 82
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 414
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 203 of 414
            Pseudo Genes (incomplete)         :: 164 of 414
            Pseudo Genes (internal stop)      :: 114 of 414
            Pseudo Genes (multiple problems)  :: 61 of 414
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277784-rid23485933.guided.282"
                     /strain="M2307618-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-10-02"
                     /collected_by="FSS"
     gene            complement(171..377)
                     /locus_tag="ACHURJ_004569"
                     /pseudo
     CDS             complement(171..377)
                     /locus_tag="ACHURJ_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611787.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
     gene            541..1098
                     /locus_tag="ACHURJ_004570"
     CDS             541..1098
                     /locus_tag="ACHURJ_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005687610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR1283182.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTD
                     REGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGD
                     MGQMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTG
                     ATEIAHQLSIARSTVYKILEDERAS"
     gene            1281..2141
                     /locus_tag="ACHURJ_004571"
     CDS             1281..2141
                     /locus_tag="ACHURJ_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="HFR1283183.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            complement(2252..3010)
                     /locus_tag="ACHURJ_004572"
     CDS             complement(2252..3010)
                     /locus_tag="ACHURJ_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin"
                     /protein_id="HFR1283184.1"
                     /translation="MTKISERWKHNGITEGYCNICGKYGLLTKDHVPPKCAITLGPVL
                     QKTVSEFFGIQEPVKPLNAKNGSYFRTICSHCNNKVLGGLDVAIENVTKSFKEQLSRY
                     MNGMNVYPFIRIPFDSISFTKAMIGHVLSATSVEDCKKEPVDSPFYTPLKDYVLGKNS
                     SFEETHDIYYWFYPHRMHISAQSVAFMNEGHVAFICALHFFPIGFIITMKNEGTYPAH
                     STKLELEDKFLTFNMTSINYEYTGSDAQWNENSR"
BASE COUNT          948 a          695 c          729 g          960 t
ORIGIN      
        1 tttcttacta ttattttacc atatatcgag tcataaaagc tgataattta acatattttt
       61 gagcactttt ctttcaccca atgggtgaaa gctgaatttc gaaggaatgc atatttatca
      121 aggctttgat tatgcttttt gaagtactga cgtagaatct aggtatgatt catatcggtg
      181 aggaaggtgc ccagaaaacg gacacatcca atttgcaggg caatgcccag acggttgtga
      241 tcacctctgc tttttccgat aaattccttg tctgcttcat caaggtgaaa atatcgtgcc
      301 agctgaagct catccggttc accggtgaat ctgccatagc tttcagtctg ctcagtggtc
      361 agaaagtcaa cgggcatatc ggcctccctg cctgacgggc atttagtaac atttttccaa
      421 ccgtacgaaa tgttataaat tatcggacat cgtaaaactg ttacattaat atgtctatta
      481 aatcgtaaat ttgtaataat agacatgagt tgtccgatat tcgatttaag gtacattttt
      541 atgcgacttt ttggttacgc tcgggtctca accagtcagc agtctcttga tcttcaggtc
      601 agagcactca aagacgcagg tgtgaaagca aaccgtatat ttaccgataa ggcatccggc
      661 agttcaacag accgggaagg gctggatttg ctgaggatga aggtggagga aggtgatgtc
      721 attctggtta agaagctcga ccgtcttggc cgcgacactg ccgatatgat ccaactgata
      781 aaggaatttg acgctcaggg cgtggcagtc cggttcattg atgacgggat cagtaccgac
      841 ggtgatatgg ggcaaatggt ggtcaccatc ctgtcggctg tggcacaggc tgaacgccgg
      901 aggatcctag aacgcacgaa tgagggccga caggaagcaa agctgaaagg aatcaaattt
      961 ggccgcaggc gtaccgtgga caggaacgtc gtgctgacgc ttcatcagaa gggcactggt
     1021 gcaacggaaa ttgctcatca gctcagtatt gcccgctcca cggtttataa aattcttgaa
     1081 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     1141 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     1201 tctaaataca ttcaaatatg tatccgctca tgagacaata accctggtaa atgcttcaat
     1261 aatattgaaa aaggaagagt atgagtattc aacattttcg tgtcgccctt attccctttt
     1321 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     1381 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     1441 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     1501 tatgtggtgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac
     1561 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     1621 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgctgcca
     1681 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     1741 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     1801 acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg
     1861 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     1921 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     1981 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     2041 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     2101 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     2161 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     2221 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     2281 cagaccccgt atactcataa tttatagagg tcatattaaa tgtaaggaat ttatcttcaa
     2341 gttctaattt tgtagagtgg gcagggtatg ttccttcatt tttcattgtg ataataaaac
     2401 cgataggaaa aaaatgtaaa gcacaaataa atgccacatg tccttcgttc ataaatgcta
     2461 cactttgagc ggatatgtgc attctgtgtg ggtaaaacca gtagtaaata tcatgagttt
     2521 cctcaaaact tgaattttta cccaaaacat aatcctttaa aggtgtatag aaggggctat
     2581 ctacgggttc ttttttacaa tcttcaactg aagttgctga tagcacgtgc ccaatcatag
     2641 ccttagtgaa agatatgcta tcaaaaggta ttctaatgaa tggatataca ttcataccat
     2701 tcatatatcg acttaactgt tctttaaagg actttgttac attttcaatt gcaacatcga
     2761 gcccacctaa taccttattg ttacagtggc tgcaaatggt tctgaaataa gagccatttt
     2821 tagcatttaa tggtttaact ggttcctgaa taccaaaaaa ttcgctaact gttttctgca
     2881 aaacagggcc taaggttatg gcgcactttg gaggaacatg atcttttgtg agcaagccat
     2941 atttcccaca aatattgcaa taaccttcag taatgccatt gtgcttccat cgttcactaa
     3001 ttttcgtcat ttttattccc tcagtagcat aagctatcta tacgtttttt aatcctatca
     3061 aagatacaac ttaacgtcag gaaaatgata gtgtttatct aagtaataca attaacttca
     3121 acgcgtgaca gtctgctttt cgctcttagc ggacattagc ataggctatt taccataacg
     3181 cctcattacg cgtaccgctc agacggcctg agcgcgtttc tgacgtatcc ccgttaaaaa
     3241 cagtaacaaa ccacccgaaa atgcacgcca gaaacgctac gtaagaatct atggcactgt
     3301 tgcaaatagt cggtggtgat aaacttatca tc
//
